LOCUS       DARBET010000001       128997 bp    DNA     linear   BCT 07-NOV-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4027
            SAMEA114569841-rid20112183.denovo.001, whole genome shotgun
            sequence.
ACCESSION   DARBET010000001 DARBET010000000
VERSION     DARBET010000001.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA114569841
            Sequence Read Archive: ERR12203666
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 128997)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 128997)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001972618.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001972618.1
            Genome Coverage       :: 132x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/07/2023 13:18:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,041
            CDSs (total)                      :: 2,975
            Genes (coding)                    :: 2,908
            CDSs (with protein)               :: 2,908
            Genes (RNA)                       :: 66
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 67
            CDSs (without protein)            :: 67
            Pseudo Genes (ambiguous residues) :: 0 of 67
            Pseudo Genes (frameshifted)       :: 26 of 67
            Pseudo Genes (incomplete)         :: 28 of 67
            Pseudo Genes (internal stop)      :: 20 of 67
            Pseudo Genes (multiple problems)  :: 6 of 67
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..128997
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA114569841-rid20112183.denovo.001"
                     /isolate="VRE4027"
                     /db_xref="taxon:1352"
                     /country="Denmark"
     gene            complement(12..1535)
                     /locus_tag="SCJ86_000001"
     CDS             complement(12..1535)
                     /locus_tag="SCJ86_000001"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000724057.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ABC transporter substrate-binding protein
                     AdcA"
                     /protein_id="HEG1167598.1"
                     /translation="MKRLIGILAMLVLAGMLTACGASGKAEDSKEKLSVMTTFYPMYD
                     FTKAIVGDEGEVELLIPAGTDSHDYEPSAKDMAKIQDTDIFVYNDENMETWVPAIQKT
                     LQEGNVHTIKATEGMLLLPGSEEGHDHDHEHGEEGHTHELDPHVWLAPSLAIKQVANI
                     RDQLIEAYPEKQEVWTKNAAAYTEKLQALHQLYQETFKQAKQRSFVTQHAAFNYLALE
                     YGLNQVSIAGLSSSEEPSAARIAELKHFVKEHGINYIYFEENAKDSIARTLANEAGVS
                     LEVLNPLEGLTNEQIENGENYLSIMEANLEALKKTTETENPLEDTLTPKKEKSVYNGY
                     FEDEDVQDRSLSDWAGTWQTVDTYVEDGTFDPVFEYKEKLNQDKTAQEYKEYYTKGYQ
                     TDIASIKISEKEMAFTFKDGTTKRSNYRYVGKEILTYEAGNRGVRYLFEAEDKESGAF
                     RYIQFSDHTIAPADSEHFHLYAGNESQEVLLKEMENWPTFYPDTMDGREIAQEMLLH"
     gene            1787..2968
                     /locus_tag="SCJ86_000002"
     CDS             1787..2968
                     /locus_tag="SCJ86_000002"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341870.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter"
                     /protein_id="HEG1167599.1"
                     /translation="MKKETDTTNRLIRFLGGKTSYYVLGIVILLALIIYFFHQISFIF
                     KPVGIIIATILPPLLFALILYYLLEPVIKRLSKHLSRNLSVIIVYVIILFLLGLGSIW
                     LIPFLEEQAKTLISSLPDLFQDFQESLRQFLEKTPFADSFNQFFASIDDVTSDIAGFI
                     GNYWESGAQRISNIFSTVTAIFITLFTGPIVAFFLLRDPQKFYQSVLAIVPPAFRTDF
                     KNLTKIANQQLGSFLKGQIIASFILGAVYWVCFLLIGLEFASVLAISAGLLCIIPYIG
                     PFIAFFPGLIIAFQDSTFMAVKFVIVWFAVQLLHGDLVIPRVMGDRLQIHPITILIVL
                     LVMGDLMGIVGVIFGIPIYTLVKLLVTFIFRKFKQRYNKFYGDKGEYENSSFSEEDYF
                     K"
     gene            complement(3028..4656)
                     /gene="treC"
                     /locus_tag="SCJ86_000003"
     CDS             complement(3028..4656)
                     /gene="treC"
                     /locus_tag="SCJ86_000003"
                     /EC_number="3.2.1.93"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286285.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha,alpha-phosphotrehalase"
                     /protein_id="HEG1167600.1"
                     /translation="MTFKDKVIYQIYPKSYKDSNGDGIGDLKGIKEKLPYLNDLGIDM
                     IWLNPIYPSPQKDNGYDISDYTAIDPIFGTMEEFEQLIAEAKKYRIEIMLDMVLNHVS
                     TEHEWFKKALAGDQHYQDFFILREEPTDWVSKFGGSAWSPFGDTGKYYLHLYDRTQAD
                     LNWRNQAVREELFKVVRFWMEKGVKGFRFDVINVIGKDEQLKNNKENDGKPEYTDKPI
                     THTYLKELNQATFGTDSTIITVGEMSSTTIENCILYTEPSREELSMVFNFHHLKVDYE
                     NGNKWSTPPFDFAELKRLFHTWGENMSEGNGWNALFFNNHDQPRALNRFVVIDQYRKQ
                     GAEMLATMIHLNRGTPYIYMGEEIGMIDPDYSSISDYIDVESLNAYKLLLEQGFTEKE
                     AFRRIKAKSRDNSRTPMQWSDTEQAGFTTGKPWLKVGGKLEEINVEKELSSEDSILSY
                     YKKLIRLRKTYPIVAQGDYHAYEANHPEVYGFLRQFEGQQLLVLTNFYDKDTRIAIPE
                     GFTEANILIDNYSDTVLHQSLELKPYQAIALLKN"
     gene            complement(5053..7764)
                     /locus_tag="SCJ86_000004"
     CDS             complement(5053..7764)
                     /locus_tag="SCJ86_000004"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333222.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEG1167601.1"
                     /translation="MKTRTYLKAGFREIRQSKGRFIAIILIVMLGTLLFVGVKTAGPV
                     MQKTMDHYVGTAGLSDLQIVSTGGLTQKDIAQAEKIPDAKVETGKQIYYSNTGKNEVI
                     QIFSYNKNNKQNKLQLTDGKLPEKPNQLVLDEKAEDEGYKIGDIYQIDSDELKEKEYT
                     ITGFVRSPLFINNLERGYANVGNGSVDYFVYLPESVFKTDIYSVLYLDFTNVKNLNTY
                     SKAYKDKMEQNQEKAEKYLQDRPEERLEELKKSANEELEPAQQKIADGKAQTTQARTR
                     LEDAKKQLAQQSATIQQLPEAQRTIAVQTLPQQEAQLAEQERQLVEKERELASAEEEL
                     NRNAEKIENLTKPSYLYNERSENVGFQEFGDLSDRIAAIANVFPVFFFFIAALITFTT
                     MTRMVEENRREIGTLKALGYTKVEIAKKYAIYASLASGIGIVLGTILGTNLLPRIIFE
                     LSNERYDIGSALIFYDWPPILQAAAAFFIAAFGAAMIVLFKDLRERPAALLQPKAPKP
                     GKRIFLEYITPLWSRLSFNQKISYRNLFRYKSRMLMAIIGIAGCAGLMVAGVGLKDSL
                     SSVSAKQFGPIIDYQAIVTLDSSTEDSRQKAADMLQKQEKVNEILAIHTQTVELRKKG
                     HASQSLTVIVPSKTADLDRFIHLTDLEENPRSLPKTGIAITQKVAELFDLSTGDHISL
                     YDEDQKEWKVEVKEIVKNYLGSFIYMSPEYYASVTDQSMMDNAFLLRTDKMTTKEEEM
                     LSEKLLATDAVTNTSFMSKQIETQEESMANLDSVVLIFVVLSGLLAFIVLYNLTNINI
                     SERVRELSTIKVLGFFDKEVTMYIVRENIIFTLLGIIGGFGVGYILTDFILQQASMEN
                     VIFPLVITWWAYVLSAGLTIVFTVIVMIVTHFKLKHIDMIDALKSNE"
     gene            complement(7774..8475)
                     /locus_tag="SCJ86_000005"
     CDS             complement(7774..8475)
                     /locus_tag="SCJ86_000005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310780.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEG1167602.1"
                     /translation="MKYVEVKNEYKRYKMGETTITANDDISFSIEKGELVIILGPSGA
                     GKSTILNILGGMDTPDEGQIIIDDTDIAQFSDKQLTAYRRTDVGFVFQFYNLVPNLTA
                     KENVELATEVSPDALDPVEVLRQVGLAHRLNNFPSQLSGGEQQRVSIARALAKNPKLL
                     LCDEPTGALDFETGKQVLKLLQNASRQHGNTVLIITHNSALAPIADRVIHINDAKVRS
                     VELNDHPSSIDEIVW"
     gene            8661..9260
                     /locus_tag="SCJ86_000006"
     CDS             8661..9260
                     /locus_tag="SCJ86_000006"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318533.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine hydrolase"
                     /protein_id="HEG1167603.1"
                     /translation="MNDLKENMIFDLSKTAFVAIDMQKEIVNNGTLSPHTAASILTNN
                     DLLVRTLKRTAALKVLVNVDISTFPYLSQQTDMGGMTASVPPEFTDLLLKDSLKDTDN
                     MLTIKKYNPSAFFGTSLDLQLRRRGIETIILSGVATTNGVYATALDAFQHGYHIVLAE
                     DACSDRDKESHQLFIKKIFPKTARVRSTKQIIEAIQQSK"
     gene            9341..10354
                     /locus_tag="SCJ86_000007"
     CDS             9341..10354
                     /locus_tag="SCJ86_000007"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325301.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="substrate-binding domain-containing protein"
                     /protein_id="HEG1167604.1"
                     /translation="MVKKKITIKEVAKLSGVSITTVSLILNGNTEKFSPKTVQKVLAA
                     KEELGYQPDYFARQMITKETKTIGVLVPDITNPFFSTLMRGIEDILYKQNFVTILCNA
                     DSDHQKEIEYLAELTRRGVDGFIIATSAVSTDAINENLKKQGRPFIVLDQKKSEGFSD
                     AVRTDDFRGGYLAGMHLLSLGHQTIALVYPENPPENVHARIEGFKSALDVYQIPHDQL
                     ILLPTQFSKQGGYQITAELLDSAATGVFALNDELAFGLYRGLEEAGKSIPEDYSIIGY
                     DNIDMCEYIKPKLTTIAQPIFELGQTSAKLLLDRIQFPEKEWEEKRLPVRLEKRFSTA
                     PLK"
     gene            10462..11379
                     /gene="rbsK"
                     /locus_tag="SCJ86_000008"
     CDS             10462..11379
                     /gene="rbsK"
                     /locus_tag="SCJ86_000008"
                     /EC_number="2.7.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307932.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribokinase"
                     /protein_id="HEG1167605.1"
                     /translation="MNTVTVIGSINMDTTLRLTNMPKPGETMHAHEIFHAGGGKGANQ
                     AVAAKRSGARTSFIGGVGADSEGQQLLDLLTKENIDTSGIAEIQGATTGQAMIMVDAA
                     GENSILIHAGANNAFHEQEVLKNKQLITNSDFIIAQFESSLDATILAFSIAKDAGKTT
                     ILNPAPARETIPTELLEKTDIIIPNETETEIITGIRVTDHNSLVAAAEKLHELGIGTV
                     IITLGSAGAFYHTEKEHGIVPAFKVDAVDTTAAGDTFIGALSSTLQPDLSNLKEAILY
                     GNLASSVAVQSYGAQPSIPYREALEQSAV"
     gene            11407..11802
                     /gene="rbsD"
                     /locus_tag="SCJ86_000009"
     CDS             11407..11802
                     /gene="rbsD"
                     /locus_tag="SCJ86_000009"
                     /EC_number="5.4.99.62"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307931.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-ribose pyranase"
                     /protein_id="HEG1167606.1"
                     /translation="MKKGKVINSDISRVIAQMGHFDKLSIGDAGMPVPFGTEKIDLAV
                     SNGLPSFLSVLENVLEELEVQSIELAEEIKTMNPEILEKIKLLLPDTPISFIPHEEMK
                     KELNNCHAFIRTGEMTPYANIILVSGVVF"
     gene            11802..13175
                     /locus_tag="SCJ86_000010"
     CDS             11802..13175
                     /locus_tag="SCJ86_000010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373274.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HEG1167607.1"
                     /translation="METTMNPEQMVLSEQIHMLIEQLGGKDNIDSVLPCATRLRIKLK
                     KQPVANAEKLKQQIPSIKYFILLDRYAEIIMDQGLAPAYKELTRQIDHSAPKEKLNPI
                     EKLFGLISEIFTPIIPLFAGSGILKGLVVLATTIGWLSETSGTYHILSAASNAVFYFL
                     PIFLAFSAAKTFKVNGYIAAVIAAALIEPNLTSLLSDSGKAATDFLGIPVVLMQYTST
                     VMPAILGIFFYSFVEKFLKKYIRENMQLVFVPLLSLVITVPLTLMVFGPAGVYLGEGL
                     AAAITWMMDINGPISGAIIAGGWNILVIFGIQWAVNPVMISNISAYGFDRIVPLTGAA
                     NFGMAGAALGVFLRSKRSKTRSISGSAFASILLAGVTEPTVYGIAIPLKKPFVAACIG
                     AAAGGAVMGFAQVKAIAFVFGSLTTLPAFISGTFFWYLAGLAVSLVVAMITTLVSGFD
                     EDLMSYE"
     gene            13532..15676
                     /locus_tag="SCJ86_000011"
     CDS             13532..15676
                     /locus_tag="SCJ86_000011"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="C40 family peptidase"
                     /protein_id="HEG1167608.1"
                     /translation="MKNKKTIRLLAATWLIAPSVLSTNTVFAVDNQIADQTVTTDTTT
                     SSSATSETTDSFTEDPTGTTNSTTGSTEPTEIDPADPSENEKPAETYSLTIAPSLIGK
                     IQLTASTADSPEQQPLDITESGTVEGLLAETKVTYTITPAEGEQIASVAVNGAKEVNY
                     DAKTFTVGTNNLSITAEFKAGPDTSTEKPTDPSEEVPSTPDPETPNKPDVGNNNNNNG
                     SNNSNNNNNNSNSGNSSNQTNPSGSNNNGNTQREPNHPNNGSSNGGTNNASSRPNTAN
                     QSGIENPSSASSDFVVKSPIDAVLPTNTTNVQQAIVREAFKHLGKPYVWGAKGPNTFD
                     CSGLTYYVYMKATGHYIGGWTGEQQYAGTQIPVSQAQPGDLVFWGPSSGVTHHVGIYI
                     GNGQFIHAPQPGDKVRVTSISDFTPDFAVRVNLAGLPAASGSLTGGSSILDGLDGSFH
                     FSQNQTTDQFLKKIADDAQEIGQKEGIYASVMMAQAILESGSGNSLLSSEPNHNLFGI
                     KGSYKGSSVTFNTLEQDSSGQSYQIRAQFRKYPSYKESLEDYADLIKNGLTGNPDFYK
                     PTWKSETKDYKEATKYLEGRYATDRQYSQKLNAIIEAYDLTKYDEPKKEAATTEEANA
                     EILNMSKRFDVPIKWTTDGVPITGLEFSTIKRVNNASSIFRVASIWDLWNNFTARQIP
                     ETTTRTVKAATVPVLTLLSLDKSFWLDLPLKK"
     gene            complement(15728..16768)
                     /locus_tag="SCJ86_000012"
     CDS             complement(15728..16768)
                     /locus_tag="SCJ86_000012"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337350.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactose mutarotase"
                     /protein_id="HEG1167609.1"
                     /translation="MEINEQNFGENYHLITITNQNGTSLSVSDLGARVVHFQTIIDNE
                     KRELILGFDEAKEYLEKDPYIGASIGRMAGRITNGTFKLDDETFEVKTDPDTGHCLHG
                     GAPSFEAKQWHYTVINGENEASVIFYQTSPDMGNGFPGTLDVEVRYTLTNDDTWRVTV
                     QGISDRKTVFNPTNHVYFNLTGDPAQSIDEHILWLNSRLFATLNKDTTPTGEVVSVSG
                     TAFDFQRPKKLGEVFASDFRQSMIVGGIDHPFLLKKQSGLLNAAELTSPDEKVSIQVK
                     TDASGIVIFTANFGEDGPEMRGNKLVNHGGITFETQELPGAERLESFGDVTLEPNQVR
                     EFVTEYKINIKK"
     gene            complement(17045..18847)
                     /locus_tag="SCJ86_000013"
     CDS             complement(17045..18847)
                     /locus_tag="SCJ86_000013"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEG1167610.1"
                     /translation="MLWKLSLTGIKSRLRDYLVLFSGLVMASAIFYMFESMASNEAFL
                     KSNTIVAATVIIFRFGSVLLGIITFVYILYANSFLMTMRQKDYAMFMMLGAKGRKIAQ
                     MIFSETFLVGIAATLVGSAVGVGLTSIVHRLLVDQLDLNISHFTPFSIHGLLITVLFF
                     AVLFLLAAVVNAFSIVKKPILALIRADQTPTRIKQHKFLFFLEVVLGIAFLGCGYYMM
                     KHVGTYQVFGIGVALVTIILGTYFIFHSVIIFFLSLLKKSEQISLKKLNNFTLSQLNF
                     RIREYTQMLSMVAMLFALALGALTVGLGFKNEIADLTENNSGYDLVMNNPRAEDQQKI
                     KELSPTLSASYTQKEDAGTIYYNASEFAAQPLIMIKHEDGTPPKITKEKVPAKKMNEL
                     TVQEELRSYELPEQQEKEIKVVSQSEFDQLNLPQSTLQLVQVKDFQANLKPIETLVAQ
                     NKMNNPSLQKVEYSNQKYEMYEVYNGLFSGFEFMGFFLGLAFLTMLASCLMFKILSGS
                     KSDIARYEMLEKIGTRRGLLKQSIRREVGVLFLAPGILGVVHVLFGLQMFGLFMQNPY
                     KDIWVPFTIFFVLYFIYYVLTTWLYTGIVLKKRL"
     gene            complement(18861..19613)
                     /locus_tag="SCJ86_000014"
     CDS             complement(18861..19613)
                     /locus_tag="SCJ86_000014"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEG1167611.1"
                     /translation="MNKIVEVQNVTKVYGKNNEKKTQALSGISFDVEKGEFIGIMGAS
                     GSGKSTLLNILSTLDKPTDGHIRINQKDVTTLKGNQLADFRANEIGFIFQDFNLLESL
                     TAQENIAVPLSLQGVRPKEIKQRVQKIAERLSISHILESYPSEISGGQKQRVAAARAL
                     ITQPTILLGDEPTGALDSKSARDLLDTMDELNTKDHVSILLVTHDPFSASYCQRILFI
                     KDGGIHQEVKRGDQSRDSFYREILSILGNLEQ"
     gene            complement(19622..19984)
                     /locus_tag="SCJ86_000015"
     CDS             complement(19622..19984)
                     /locus_tag="SCJ86_000015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344598.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YxeA family protein"
                     /protein_id="HEG1167612.1"
                     /translation="MKKLLIGLLVFIVVVLAGVKIADYVVMGGEEYYVQITTDGTKTV
                     SKSDSGQETVNYRYILPGYTKEGKEKELDFKGQLTRPLRKGAYLKITWNKNKGVTSYE
                     EVEKKDVPKTALEKLIKG"
     gene            complement(20160..21032)
                     /locus_tag="SCJ86_000016"
     CDS             complement(20160..21032)
                     /locus_tag="SCJ86_000016"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SPFH domain-containing protein"
                     /protein_id="HEG1167613.1"
                     /translation="MREKRAFYINGYAGIVGLVVLALVGLFFFYLGMWQAKVWALFLS
                     IFLWLITLLLLSSATVVSPNQAKVILFFGQYLGTIRENGFFLTIPLAQKMTVSLKVRN
                     FNSSVLKVNDLDGNPIEISAVVVFKVVDTAKALFDVAYYQDFVEIQSETAIRHIASQY
                     PYDTFNENDLTLRGNTTAVSDELQKELQERLAVAGVEVIETRLNHLAYATEIASAMLQ
                     RQQAKAILSARQTIVEGAVTITQMALEQIEDGQEINFTDDRKVQLINNLLVSIITDKG
                     TQPVINTGDMKDRK"
     gene            complement(21058..21570)
                     /locus_tag="SCJ86_000017"
     CDS             complement(21058..21570)
                     /locus_tag="SCJ86_000017"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323233.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5348 domain-containing protein"
                     /protein_id="HEG1167614.1"
                     /translation="MKVEEGQREKLKTEMERLHTYITQLSQTFYDPDKEKVMVNYPNN
                     SEGRQLEQVYHEVFKHLLTVKKELDYYSLPIIDTGILKYDGKKERFIFKSVREDLPLS
                     AGMDLEVLVEDYFTEEKHWVRTKLDYLPQAAGGTQARGWYITEDKELELEGVMARIRK
                     KIKKVTLDIY"
     gene            complement(21708..22499)
                     /locus_tag="SCJ86_000018"
     CDS             complement(21708..22499)
                     /locus_tag="SCJ86_000018"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4097 family beta strand repeat protein"
                     /protein_id="HEG1167615.1"
                     /translation="MLTGGLLAGATYTLGAQKSLTWDNGPRLIDMVTETKKIKDANKI
                     VIHAENQTVSVIRGLDFEIKTTYDKTEKPEIKEKNGVLTVNAVKKSTGAIISLEDTSA
                     QITIVVPADHELSELTIEGNNSAIDINEVSIKKVNTKTHDGWVSFNEFTGGTITAKDE
                     LGSLRLDEANVDNLIVTGENSNFHLEDSLISKEGKVILNGGNLSLHEMENSGYTIQTS
                     GESYIEKDYQPITNTLLEQGQKKLKITAKDTSINITTDQEDNETD"
     gene            complement(22554..23162)
                     /locus_tag="SCJ86_000019"
     CDS             complement(22554..23162)
                     /locus_tag="SCJ86_000019"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328762.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1700 domain-containing protein"
                     /protein_id="HEG1167616.1"
                     /translation="MNEYLNEVVDHLQEIPEDDRFDLIQYYEEYFLDSGKTLDQIIDE
                     YGTPKQFALKLKISYFSEEEQAENELVKNPKRQIRLIWLIIIGLFASPVLIPLALGFI
                     LVIGGLLVALLALIFSIYVLCISILGVGLFMIISGISVFGQSILSGLFFIGVGLFATG
                     SAVFISPLLLKGTKGLFYVMMNFVKWMGRRFITKRDLHSAGV"
     gene            complement(23155..23472)
                     /locus_tag="SCJ86_000020"
     CDS             complement(23155..23472)
                     /locus_tag="SCJ86_000020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286315.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PadR family transcriptional regulator"
                     /protein_id="HEG1167617.1"
                     /translation="MHIQIPTVLLDGTVLAALSKEDMYGYALTKIVQESLSVSESTLY
                     PVLRRLKKEGYLETYDEPFEGRNRRYYSLTPMGSRHLEEIIDEWIVFRDSLDRLLEES
                     TNE"
     gene            complement(23628..24356)
                     /locus_tag="SCJ86_000021"
     CDS             complement(23628..24356)
                     /locus_tag="SCJ86_000021"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF554 domain-containing protein"
                     /protein_id="HEG1167618.1"
                     /translation="MPTGVLINVGSVLLGGLIGGLVGNKLSEHFKAQLTMVFGVCSMG
                     MGIYSIAPMKNMPAVIFALVIGTAIGLIVHLGNGINKGAALMQVPISKIFPSEKLGMT
                     HDEFISTLVTVIVLFCASGTGIYGSLDSGMTGDSTILISKSVLDFFTAAIFACNLGYV
                     VSVIAIPQFIIFYILFLLAKFIYPLTTPDMILDFKACGGFLMVATGFRMINVKMFPVA
                     DMIPAMIVIMPLSWMWTNWIMPLL"
     gene            24515..25396
                     /locus_tag="SCJ86_000022"
     CDS             24515..25396
                     /locus_tag="SCJ86_000022"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297410.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HEG1167619.1"
                     /translation="MDIHKFEVFLDLAETMNFTKTADRQFTTQGNISKQILSLETELD
                     VKLFERAHRKIELTEAGSLLLPYVKNVVEQYHAMQEVLETYTKDNNLSLNILTIPTMI
                     NYKGFSKITEFLKRHPEFTVQLKEVESVELTYNDANEHSDTIHFARNFQAPPSSIEWL
                     PTETDDFVAVLPKNHPLASKKKLDLSELKKENFLLLGPTTNLYQPVLDLCREAGFEPK
                     ISYKGARIDLIINMIANDMGIAIVMEKTVKNLLKENTVILPISPTTESYLAFTRKVGE
                     HSLASDTFWEYLNQEEN"
     gene            complement(25633..26676)
                     /locus_tag="SCJ86_000023"
     CDS             complement(25633..26676)
                     /locus_tag="SCJ86_000023"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296820.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing histidine kinase"
                     /protein_id="HEG1167620.1"
                     /translation="MKKKLFSQLWIYFASIVFISILVTLLCFLGFIFLLSRHSISPAE
                     SAGKPTLYPLFVFAGFSMVVGTGISIFVGRRILHPISALGTNMSLVATGDFSIRMDEQ
                     QKVAEVQQLYKDFNVMVQELNSIETLRNDFVSSVSHEFKTPLATIQGYVQLLQAPNLS
                     DEERQIFLYRIIESITQLSQLTENILKLNKLENQRIQLEKKEYRLDEQIREVIVFLQP
                     KWEKEQLELDIELAAVNYTGNEEFLYQVWLNIMDNAIKYNQINGQIHIKLFETATEIG
                     LEVTDSGVGMNEETRDRMFEKFYQGDTSRQISGNGLGLSLVKKILELHDGRIDYSSIE
                     GVGTTAMIRLSKQ"
     gene            complement(26679..27347)
                     /locus_tag="SCJ86_000024"
     CDS             complement(26679..27347)
                     /locus_tag="SCJ86_000024"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315931.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HEG1167621.1"
                     /translation="MNRILVVEDDPQLQLLYRSVLERAGFSVITALNGEDALKKLEAT
                     RIELIITDIMMPKMDGYDLLDSLRSTRFDTPVLMITAKADFEDKKRGFKLGADDYMTK
                     PIDVNEMILRVEALLRRSKINYSSSLIIGQTKLELDAYTVLQDQHSLILPQKEFLLLY
                     KLLSYPNKIFTRQQLMDDIWGLDTNTEERTIDVHIKRLRTRFEKTEDFQIITVRGLGY
                     KAVI"
     gene            27489..29825
                     /locus_tag="SCJ86_000025"
     CDS             27489..29825
                     /locus_tag="SCJ86_000025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345400.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HEG1167622.1"
                     /translation="MLQLKDIKKYYKVGETTTKALDGVSVAFRQKEFVAILGPSGSGK
                     TTMLNVIGGLDNYDSGDMVINGKSTKDFKDSDWDAYRNNSIGFIFQSYNLIGHLGIIE
                     NVELGMTLSGVSKDEKRKRAEDALHRVGLTDHMHKKPNQLSGGQMQRVAIARALANDP
                     DILLCDEPTGALDTETSIQIMELIQELSKEKLVIMVTHNPELAHQYADRIIEFSDGKI
                     LTDSHPHIERPKDDQFNLRRTKMSFWTALKLSFNNIRTKKGRTFLTSFASSIGIIGIA
                     IVLSLSSGFQKQIDNTQAETMAKFPITISKVTTNQTRDDAGLGASKADYPDSKTITAK
                     VSEEDKAQHTNKIDQTYVDYVTDIDPNLSNNIGFTRTTGINLLRDVNGKVQPVSFSNQ
                     NPDTESLSLSSTMSAMTGVGVSSFPTQLDTSKENFLKDNYSLLAGSYPASATDVVLIV
                     DGNNNTNINALKNLGFDVKEDEKLDFDDIVGTTFKLVNNNTYYTKLPTGNFIPNTDYD
                     AMYQNASDELKISGILRVKSSSTMNLLSPGIAYSDQLTTQIVNENKESEIVKAQRDSG
                     VNVLTTEKVDENAKQTLLSYLGGDSLPSSIMIYPNNFEDKEKILDYLDDYNKGKSDED
                     KIIYTDLAGTMTELTGGLMDAITYVLIAFAGISLVTSMIMISIITYTSVIERTKEIGV
                     LKALGARKKDITRVFDAETCILGISSGILGVFIAWLATFPINSILYNMTDLKNVAQLN
                     PVHAIILVIVSTILTMLGGHLPARMAAKKDAAIALRAE"
     gene            complement(29940..30251)
                     /locus_tag="SCJ86_000026"
     CDS             complement(29940..30251)
                     /locus_tag="SCJ86_000026"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333221.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEG1167623.1"
                     /translation="MKKMKESFPKAVKVENIANILKVTFENGEVKYVKSHWTEEITDA
                     LQFGKKGRGKRKNLLALSRNMWIGTEVTIEADGTVFINGKDRYTPEELWYKGKKSIPE
                     L"
     gene            30432..31907
                     /locus_tag="SCJ86_000027"
     CDS             30432..31907
                     /locus_tag="SCJ86_000027"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286329.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UbiD family decarboxylase"
                     /protein_id="HEG1167624.1"
                     /translation="MTDQPYDLRKVIEELKTLPGQYHETDIEIDPEAELSGVYRYIGA
                     GGTVKRPTQEGPAMTFNNIKGFPGTRVNIGTMASRKRVGEILHHDYRTLGRFLKDAVE
                     NPIKPVKVDKADAPAQEVVHLATDADFDIRNILPAPTNTPEDAGPYITMGVVLGSDPE
                     KTMSDVTIHRMVLEDKDTIGMYIMPGGRNIGHFQKQFEALNKPMPITINIGLDPAIAI
                     GTTFEPPTTPLGYNELWCAGALRNEPIQIVDGIAVDEVGIARAEFIIEAEIMPNETMQ
                     EDINTNSGKAMPEFPGYTGDANPAVQVVKVKAVTHRKDNPIMQTTIGPSEEHVSMAGI
                     PTEASILALVDKAIPGKVVNVYNPPAGGGKLMTIMQIRKENEADEGIQRQAAILALSA
                     FKELKTVILVDEDVDIFDMNDVVWTFNTRFQGDKDIIVIPGMRNHPLDPSELPEYNPG
                     SIRFRGMSAKTIFDGTVPFDMKEHFVRAKFQEVEDWEKYLK"
     gene            31954..32538
                     /locus_tag="SCJ86_000028"
     CDS             31954..32538
                     /locus_tag="SCJ86_000028"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297414.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UbiX family flavin prenyltransferase"
                     /protein_id="HEG1167625.1"
                     /translation="MEQLAQRKKKIVIGVSGASGTIYAINLVKKLKEYPMIETHGIIS
                     PWAKQNLKLESDLSLTEFESLFDYHYSNKDLGAAVASGSFLTDGMIIVPASMKTVAGI
                     SVGLGDNLIARAADVTMKEQRKLVIVPRETPLNAIHLENLTKLARLGVQIIPPIPAFY
                     NQPQTIQDLVNHHTMKLLDQFGIVNEHSKRWQGD"
     gene            32540..32959
                     /locus_tag="SCJ86_000029"
     CDS             32540..32959
                     /locus_tag="SCJ86_000029"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312785.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid decarboxylase"
                     /protein_id="HEG1167626.1"
                     /translation="MEKETIVEVTKLGFTIQAAVKVIGKDLLITLTGGDTPHIGTVTT
                     FSRDTEMQTVRFPSHDGRFHKDDVLAEKIAVIIKTAVPGSCTITSGIHVDHISNAQIN
                     ASFPMSEELGYKILDWLEKIDFHIKEPVYYKDGEKTL"
     gene            complement(33240..33524)
                     /locus_tag="SCJ86_000030"
     CDS             complement(33240..33524)
                     /locus_tag="SCJ86_000030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328758.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1905 domain-containing protein"
                     /protein_id="HEG1167627.1"
                     /translation="MREIYSFESKIYASEVDKGGAYIIFPYDIRKEFGAGRVKVQATF
                     DNMPYEGSIVNMGVKNPDGSVCYILGLRKDIRKNIGKEIGDIVAVTVKQK"
     gene            complement(33876..34586)
                     /locus_tag="SCJ86_000031"
     CDS             complement(33876..34586)
                     /locus_tag="SCJ86_000031"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337353.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4811 domain-containing protein"
                     /protein_id="HEG1167628.1"
                     /translation="MIIGVLIVSLLAFALTNVFAKKTWQTFLSLIFGLIFVASLSLIV
                     ANLSNHFGMEKVTETKTEKIVSSADSQGADMLLYKALGNGKEKVYLYRTNEKQEKPKA
                     TGTDNETNKVEKTDGDAEKVTKTTYWEYKNDMYKFWFNIADNNHEYDSRVNTFKIPET
                     WVELSTDQAAKLAELVKKQQSTMESEAKAYVQDGMVKAMTENPKMSKAEQEQRTKALA
                     AEFQQQAFAKLVKEAKGE"
     gene            complement(34583..36055)
                     /locus_tag="SCJ86_000032"
     CDS             complement(34583..36055)
                     /locus_tag="SCJ86_000032"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008269079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MFS transporter"
                     /protein_id="HEG1167629.1"
                     /translation="MSNNQPVDIYGKPYNRTLLVVVLLIGTFCTVLNQTLLTTAFPTL
                     MKAFDISASDVQWLTTGFLLVNGIMIPITAWLINKFSSRNLYLSAMTIFLIGTITCFT
                     APNFSTLLIGRLIQACGVGVSMPLLQNIMLSIFPPEKRGSAMGMSGIVIGLAPALGPT
                     LSGYIIDNYHWRDLFGMVLPIVVLVLVLAFFLMKSVIPLSNPSIDILSAILSTIGFGS
                     LLYGFSSVGDDGWGSLKVIGFLVVGVIFIALFSWRQLHLEHPFLELRVFKSSTFTIAA
                     VLAGVTNMAMVGAEMVVPLYIQNIRGESAFHSGLMLLPGALIMGAMMPVTGAIFDKYG
                     AKRLAITGMFILTAATAPFAFLTKTTPVLYIVILYAIRMFGISMVMMPVTTSGMNALP
                     NNLISHGTAVNNTFRQIASSIGTAILISVLTNVTKANIPSKSVLDNTPLAYKDQAINA
                     TLSGYHAAFIVAIIFGILGFIIAFLLKKKEPVLMKAGDNA"
     gene            36193..36666
                     /locus_tag="SCJ86_000033"
     CDS             36193..36666
                     /locus_tag="SCJ86_000033"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319555.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
                     /protein_id="HEG1167630.1"
                     /translation="MVGASLKRLLENDQLVVGISELSDIVGVSPRQLRYWEQKGFIRS
                     IEADANCPRKYRLPTVIKVELIKKYLDDGFTLSKAAEKAEIRQKKMHHVRKVFSKAIK
                     DIELIDERYTILQIGNFKEENAKMYIIHDNETDELTYKLLPVDEKADYEQLLKEL"
     gene            complement(36819..37370)
                     /locus_tag="SCJ86_000034"
     CDS             complement(36819..37370)
                     /locus_tag="SCJ86_000034"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PadR family transcriptional regulator"
                     /protein_id="HEG1167631.1"
                     /translation="MARKNTLRYILLGLLSKKKMTGYELNQSFKNEIGEFWQAKHSQI
                     YPELAKMEEQGIIQHQVEITGEKLEKKVYELTEEGKEQLAEWIHTPTNELPVNRDEFV
                     LKLYFVKDVNDPALVEIIQQQRDLHEEKRLHLLERKKTIFPTEKEKHENYGHYLILAH
                     AINRETEYANWLTDVLEESNRKK"
     gene            37475..38005
                     /locus_tag="SCJ86_000035"
     CDS             37475..38005
                     /locus_tag="SCJ86_000035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307921.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phenolic acid decarboxylase"
                     /protein_id="HEG1167632.1"
                     /translation="MKEFKELEDFIGTHFIYTYDNGWEYEWYAKNDHTVDYRIHGGMV
                     KGRWVKDQEADIVKLTDGVFKITWTEPTGTDVALDFMPNEKKLHGMIFFPKWVEEHPE
                     ITVTFQNEHIAEMEAAREKYETYPKLLVPEFATITYMGDAGIDNEDVISEEPYDGMPD
                     DIRSGKYFDENYKRVK"
     gene            complement(38488..38931)
                     /locus_tag="SCJ86_000036"
     CDS             complement(38488..38931)
                     /locus_tag="SCJ86_000036"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEG1167633.1"
                     /translation="MIRTTTKLSAKELESVLSIWLHANLDSHEFIPSTYWENQVESVR
                     KALPDAVIYLAEIDQEIIGFAGVIDQFIAGIFVKREYRQQRIGTQLMKKIKETNKTLT
                     LTVYQKNQSAIHFYLSQGFTIQTQQKDEAVNEEELVMKWNQNADF"
     gene            complement(38947..40107)
                     /locus_tag="SCJ86_000037"
     CDS             complement(38947..40107)
                     /locus_tag="SCJ86_000037"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286364.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class C sortase"
                     /protein_id="HEG1167634.1"
                     /translation="MKQSKQKQSRRLLFRLFLLICIVVGLLIALYPFYVDSLNSFIDQ
                     KRIEQVQKRTAAEIEVQRKKMEEKKQRLTDQGFNPGTDPFNEKNRNESTTSSQLEEWL
                     IGSINIPKIQLNISLYDRLNGRILENGAGVLQGTSFPLGGNSTHSVISAHSGLPNRRL
                     FTELDRLENGDTFILTVLGEKLAYQVKNIQVVLPDDTSVLTISEGEDLVTLLTCTPYM
                     INTHRLLVTGHRIPYNESVKKEEGKGNQERTMRQWFILAGTIIAVVILLVFIGRLIYQ
                     YRLSKKVLDFSFIISDSSGKPVNGSSFILKHKKKTLTRNGVPFSVQSDHHGKVKLDQL
                     PGGTYRIVSADDPKVAVSFGIRKVKQEKIYFFEGRKLVKELQKNGFYFKINE"
     gene            40614..42314
                     /locus_tag="SCJ86_000038"
     CDS             40614..42314
                     /locus_tag="SCJ86_000038"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376521.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEG1167635.1"
                     /translation="MMNLSKVCKGVRQLKKYGPFLAIFFIALAFLMPHLFTHKLILGA
                     DAPFHYNRFYDAAQQIKYGDFHYFPSLYGFQQSGRIVNAVYGPFFAYLQGLIVLLAGS
                     WFHYQILSNFILYLISGFSMLILLRYVNVRNWVSVPVAVLFMSTYSIQYWSINQGFTS
                     WGTCFYPLCMIPIADFVLHKKFPVIKVAVSVGLMTQIHLLSSIMLALMYIPFYLYYFF
                     NQTEKGKALLQLIKSVLLYFVLTANIWAGLAIVYSGNKILPPFVNREMSQKAIDLDGS
                     YWLHYPKAFPILILSGFLLFIFFQQKNTLFQKIVLYTSLVFFFLSTSLIPWTTLIEKN
                     VPFISLIQFPFRFFVPFTLLLLLYLALSLNQWSEQKWFRLLSIFGILIFSAQTIQNLY
                     QHLEKWENETFVSRHTYLFDTPEEARKTFFSKDMSTSLFVFQKTTPDYLPIYKETKDN
                     KYDRYNEEILENETTFIKTHPGGLLTIKWKNTGDKTVHIPVIVYDRTVLQQNGKTLTD
                     YEVTDIGTPIVKQKKGINELTLHYQTPIYFYFILSLTLIGWFTLLCLFIYHRYKLLRA
                     "
     gene            complement(42416..43249)
                     /locus_tag="SCJ86_000039"
     CDS             complement(42416..43249)
                     /locus_tag="SCJ86_000039"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326446.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease/exonuclease/phosphatase family
                     protein"
                     /protein_id="HEG1167636.1"
                     /translation="MKFMTLNTHSWLEPEPEKKLQELADKILLEDYEVIALQEINQLL
                     ESEEVEPAKLMKFCQVKNQVPIRKDNFAYRLVQLLKEHGKEYFWSWEMSHIGYDKYEE
                     GNALLTKKTLESQVLTVSQSQKKEDYQTRKILIGKTKIDDQDIFVSSCHFSWWTDKKS
                     GFYFEWKNLENYFLETRVPLFFLGDFNNPVDSQGYYTVRESCLLLQDSYVVANEKGKA
                     ATVEKKIDGWEQNTEKLRIDFIFVPEGMQVKKYQRIFDGIDSPIISDHYGVEIELDVN
                     E"
     gene            complement(43553..45718)
                     /locus_tag="SCJ86_000040"
     CDS             complement(43553..45718)
                     /locus_tag="SCJ86_000040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353586.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HEG1167637.1"
                     /translation="MKKIFSFEFWQKFGKALMVVVAVMPAAGLMISIGKSIPLINPDW
                     TPLVTTGGVIENIGWAIIGNLHILFALAIGGSWAKERAGGAFAAGISFILINRITGAI
                     FGVTSDMLANEDAFTHTLFGTKIMVKGFFTSVLEAPALNMGVFVGIIAGFVGAMAYNK
                     YYNYRKLPDALSFFNGKRFVPFVVILWSTIVALILAVVWPNVQAGINNFGLWIAESQE
                     SAPILAPFLFGTLERLLLPFGLHHMLTIPINYTQLGGSYEILSGAQAGTQVFGQDPLW
                     LAWATDLVNLKGAGDMSQYEFVLHNWTPARFKVGQMIGSSGILMGMAFAMYRNVDPDK
                     KARYKSMYFSAALAVFLTGVTEPLEFMFMFAAVPLYVIYSVIQGAAFAMADILPLRVH
                     SFGNIELLTRTPLAIKAGLGGDLINFVLCVIAFGVVTYFLANFLIKKFNFATPGRNGN
                     YDNDSEETVVSNSGTGTADQQVVQIIHLLGGKENIKDVDACMTRLRVSVNDREKVGTE
                     EAWKRAGAMGLIVKDNGVQAVYGPKADVLKSDIEDLLQSGAEIPEPEMVSTGNGQAGS
                     KQYLGIEQELVSAASGEVIPLSEVKDPVFSQKMMGDGFAVIPTSREVVAPIAGKVTSI
                     FPSKHAIGLETKDGIEVLIHMGIDTVQMNQPAFEIAVKEGQEVGTGTKLAEMDLDVIQ
                     NEEKDPTIMIVFTNDKVEEVVIKQLGTVTAGKVIGSIKM"
     gene            45978..48272
                     /locus_tag="SCJ86_000041"
     CDS             45978..48272
                     /locus_tag="SCJ86_000041"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319549.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 65 protein"
                     /protein_id="HEG1167638.1"
                     /translation="MKQIKRLFAIDPWKISTTQLDKKNLRLQESLTSIGNGYMGMRGN
                     FEETYSGDHHQGTYLAGVWYPDKTRVGWWKNGYPEYFGKVINALNFIAIDISINDQLI
                     DLASLEPEDFYWELDMKNGVLSRTFTITTSTNKVRISFERFLSIVKKESAYIYLTIEM
                     LEGSGTVKLVSKLDGNVQNEDSNYDEHFWNEIDRGTIEEIGYLTTKTIPNNFGIEQFT
                     VTAAMRHFVNGKSVIPDYHEDTLALHGCLSAELSQGETWSVEKEIIVLTSRDIPEAQQ
                     AEKAVSELTNQTNNYIQAKEEQTAAWAERWKLADVVIEGDEEAQQGIRFNLFQLFSTY
                     YGEDERLNIGPKGFTGEKYGGATYWDTEAYAVPLYLALAKPEVTKNLLKYRHNQLPQA
                     IHNAQQQGLKGALYPMVTFTGVECHNEWEITFEEIHRNGAIAYAIYNYTNYTGDTEYL
                     KNEGLEVLAEIARFWADRVHFSKRANKYMIHGVTGPNEYENNINNNWYTNTIAAWVLR
                     YTRENYLTYQKEAAITISEEELAKWKDIAENMYFPVDEELGIFVQHDTFLDKDLMPVS
                     DIPTSDLPLNQHWSWDKILRSCFIKQADVLQGIYFFNDTFTKEEKQKNFDFYEPMTVH
                     ESSLSPSIHSILAAELGMESKAVEMYQRTARLDLDNYNNDTEDGLHITSMTGSWLSIV
                     QGFAQMKTAHDQLRFAPFLPKTWKAYSFHINYRGRLLFISVTDEQVTFELLEGFPLEI
                     YVYEQSYSLADRLEITLRKEEANV"
     gene            48265..48930
                     /gene="pgmB"
                     /locus_tag="SCJ86_000042"
     CDS             48265..48930
                     /gene="pgmB"
                     /locus_tag="SCJ86_000042"
                     /EC_number="5.4.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286375.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-phosphoglucomutase"
                     /protein_id="HEG1167639.1"
                     /translation="MFKGVLFDLDGVITDTAEFHYRAWRKLGQEIGITIDHTFNEQLK
                     GVSREDSLSLLLAYGGKEHSFSKEEFAELAKRKNDYYLEMIQTIEPKDVFPGVVPLLD
                     SLKEEKIKIALASASKNGPFLLEKMGLTPYFDAIADPAEATNGKPAPDIFLLAAKAVG
                     LKAEECIGIEDAQAGIQAILSSGAQPIGVGRAEDLGEEIPLVPDTTFLTIDYLKEKCH
                     DHG"
     gene            48923..49930
                     /locus_tag="SCJ86_000043"
     CDS             48923..49930
                     /locus_tag="SCJ86_000043"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002316657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactose mutarotase"
                     /protein_id="HEG1167640.1"
                     /translation="MDNPEISVVPVPETEIKKITLKNDYLEVVLLNYGARLHQIFAPD
                     KEGKSENVLLSYDRFEDVLTDKSFFGATVGPVAGRIRDASWGEHRLEKNCGSHHIHGG
                     TKGWSFQFWEVEVFKTPSSIGVVFYLKDEFSTYPGPITATITYRLTKNELEMTTSASS
                     LVETICNPTNHAYFNLSGNGTRDIYEHQLTVFLDGVLELDQEKLPTGNWTKKEKLPID
                     FRKSPTLQEILACYPDGLDDVFLLHHPRLSKTSLQLFEKHSGRQMTIATSNKSMVLFS
                     TTGFEADFSVNGKQMHSNYGLAIEPQEIPDIVHFPKFGSINLHPGQERISQTIYRFSA
                     Q"
     gene            49952..50971
                     /locus_tag="SCJ86_000044"
     CDS             49952..50971
                     /locus_tag="SCJ86_000044"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HEG1167641.1"
                     /translation="MAITVKDVAKKAGVATSTVSRVINDHPSISETTKKKVRKIMDEL
                     GYVPNMTARNLGKRISSAIGVILPPLDSKERIGNPFYLEIMEAINEEARNYDMTTAIA
                     TAKNFELLLENVQRMHLQKQVDGFILVYSDSQDPVIDYLYQNHIPFTLIGQPYHHENE
                     IVYVDNDNQLLGKQATEFLIQNGHKNILFVTNTTHESLYFERYFGYQKAMMMAGLPAL
                     SSVVLEHPEDYLSFEKTLKENEATALVVIDDIFALRTMQLAQMYGYVIPETLSVISFN
                     NSIFSTLTHPYLTSIDIDISELGRVAMQKLRELIHEELSSGVRLVIPHRLIKRETVVP
                     LENPY"
     gene            complement(51252..52619)
                     /gene="nhaC"
                     /locus_tag="SCJ86_000045"
     CDS             complement(51252..52619)
                     /gene="nhaC"
                     /locus_tag="SCJ86_000045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Na+/H+ antiporter NhaC"
                     /protein_id="HEG1167642.1"
                     /translation="MNKNISLKESIAVLIVLLALLGVLIIGFQLSPHIPILAAFMCLL
                     FYGRFKKFTWDEIHDGIIKGITPGIIPILIFLLIGVLVASWILSGTIPTIMVYGFKLV
                     SVRFFLPTAFLVCAIVGVTVGSSFTTISTIGIAFLGIGHLLGFNDAMTTGAVVSGAFL
                     GNNCSPLSDTTNLAAGIGGVNLFEHIAGMRYTDFPAFVLSLIFYTILGHNSHSADLAS
                     INEMIQNMKASFWISPWTLIPLVILFGGAIKKIPAIPTLLAGSTAALVLSFIHEKSLT
                     VGKAADILMNGYVANTPDPKLDELLSRGGIMSMLGSASLILLALALGGLLIKYLIVDT
                     IVQELKEKMDRPSRLVGFTALSCIGINLIVGEQYLSIILPGETFKRSFDDVGLSKTYL
                     TRTLADSGATVNSLVPWGVSGTFIMGTMKVSALHYLPFVFFSILAPVFTILGGFLLNR
                     RRVKN"
     gene            complement(52729..54150)
                     /locus_tag="SCJ86_000046"
     CDS             complement(52729..54150)
                     /locus_tag="SCJ86_000046"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328750.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HEG1167643.1"
                     /translation="MSKSEVSKMGLGTFIGLTMALCATVRSIPTLAAVGWTLIFYSIF
                     AVLFFAGPISMVSGELSTMLPQEGGPQLWVKTALGSKWGFVVAWLLWVQMFPGMVMVA
                     STLGPLLGNTFGNVELGNNHWFVLGCILVIYWIITILNLKFDMAKVGGNIGVWLGVYI
                     PVVIMFVLGVLAAFKVGLVSNGYLGDFSWSKAFPDLEHIDSLKYLAGITFIFVGIEMS
                     SVYMPRLKDATKNYTKGVFIALIGLVLLNVINAMLVANVVPDGKMELANITQPILIDC
                     QILGLPEVIGNIFSFMVFIGVLLQLSAWVTGPSKTIIQVAREGFLPPKFGFHKENKYG
                     VSRNVVLTQSIVISLFALLYGVMDDVSAVFLTLTNATTVIYCIVYILIAVSLLKMRKK
                     HPEFERPYRIGKNGNGLAWVVSCMLIFSIIVVVFATLGTATLSDALLVAAITVVMFVI
                     PLIINHFKKDSWGIEVEKSLEEK"
     gene            complement(54393..56270)
                     /gene="tdc"
                     /locus_tag="SCJ86_000047"
     CDS             complement(54393..56270)
                     /gene="tdc"
                     /locus_tag="SCJ86_000047"
                     /EC_number="4.1.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286400.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine decarboxylase"
                     /protein_id="HEG1167644.1"
                     /translation="MSESLSKDLNLNALFIGDKAENGQIYKALLNELVDEHLGWRQNY
                     MPQDMPIITPEEKSSASFEHTVNKTKDVLSEISARMRTHSVPWHNAGRYWGHMNSETL
                     MPSLLAYNFAMLWNGNNVAYESSPATSQMEEEVGMEFAKLMSYKDGWGHIVADGSLAN
                     LEGLWYARNIKSLPLAMKEVTPELVAGKSDWELMNLSTEEIMNLLDSVPEKIDEIKAH
                     SARSGKHLEKLGKWLVPQTKHYSWLKAADIIGIGLDQVIPVPVDHNYRMDINELEKIV
                     RGLAAEKTPILGVVGVVGSTEEGAIDGIDKIVALRRVLEKDGIYFYLHVDAAYGGYGR
                     AIFLDEDNNFIPFEDLKDVHYKYNVFTENKDYILEEVHSAYKAIEEAESVTIDPHKMG
                     YVPYSAGGIVIKDIRMRDVISYFATYVFEKGADIPALLGAYILEGSKAGATAASVWAA
                     HHVLPLNVTGYGKLMGASIEGAHRFYNFLNDLSFKVGDKEIEVHPLTYPDFNMVDYVF
                     KEKGNDDLVAMNKLNHDVYDYSSYVKGSIYGNEFLTSHTDFAIPDYGNSPLQFVNQLG
                     FSDEEWNRAGKVTVLRASVMTPYMNKEEHFEEYAEKIKAALQEKLEKIYADQLLASEA
                     K"
     gene            complement(56561..57817)
                     /locus_tag="SCJ86_000048"
     CDS             complement(56561..57817)
                     /locus_tag="SCJ86_000048"
                     /EC_number="6.1.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286405.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine--tRNA ligase"
                     /protein_id="HEG1167645.1"
                     /translation="MNIIDELTWRDAINQQTNEERLRELVEEKSISLYCGVDPTGDSM
                     HIGHLIPFMMMKRFQLAGHHPYILIGGGTGTIGDPSGRKTERVLQTMEQVQHNVDALS
                     NQMRKLFGKDANITFVNNYDWLSKISLLEFLRDYGKNFNINTMLAKDIVASRLEVGIS
                     FTEFTYQILQSIDFLHLHKTYDVQLQIGGADQWGNITAGLDLIRKLEGPEVEAFGLTI
                     PLMLKADGTKFGKTAGGAVWLDPKKTSPFEFYQFWLNQDDRDVVKYLKFFTFLSQEEI
                     EDLAKKVETEPEKREAQRRLAEEVTRFVHSEEDLKEAQKITQALFSGNIKELNAEEIA
                     QGFGKMPNVEISSTPENIVELLVSTKIEPSKRQAREDVSNGAISINGDRVTDLNFVIN
                     PSDEFDGKFVVIRKGKKNYFLAKVID"
     misc_binding    complement(57929..58134)
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00230"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="T-box leader; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="tRNA"
                     /db_xref="RFAM:RF00230"
     gene            58397..59698
                     /locus_tag="SCJ86_000049"
     CDS             58397..59698
                     /locus_tag="SCJ86_000049"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292598.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide deacetylase family protein"
                     /protein_id="HEG1167646.1"
                     /translation="MEKKQKIILGATSALTIVILILSLFFVIRSSTKKEPIIPEKKIT
                     EKAKEPTDDKQQLVTQAKNLAQSYDYDQAIALLEKDSKNGSTETQQLLQTLKKEKEQL
                     IDWSDPTQISHVFFHSLIVDPQKAFHSQQAQGYKDYMVTVEEFNRSIEQLYQNDFVLV
                     NLNDLIQKDEQGNLSFNGLALPEGKKPLVLSQDDVSYYEYMDNSGFPSRLVVSNNNEI
                     KNVYKEKDKEEIGDFDMVPLIDAFVKKHPDFSYRGAKGTLALTGYNGVLGYRTSKSQY
                     GDNEKTKKEIKEAKKVAEQLKKDGWSFASHTWGHLNMTQASLEDVKKDNELWQQEVAP
                     ILGKTNILIYPFGADISDWQPYSAENQKFAYLKSQGFDIFCNVDASTPAWGQLGTDFY
                     RNARINIDGIRFQSDLDGKNPILDPFINVHDVYDKEARESA"
     gene            complement(59767..60366)
                     /locus_tag="SCJ86_000050"
     CDS             complement(59767..60366)
                     /locus_tag="SCJ86_000050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328748.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HEG1167647.1"
                     /translation="MNGFEKRAQEKKKEILQAAFDLMNTDIGTAGVTMDKVAENAQVS
                     KATIFKYFGSKEGLIYEVFFAFMNDLGQEAKALIAENLPFEETLIAMSENKIRYLNRV
                     NKQFYIDLMTYYTKKEKKEFSDAMDAYTQESFSIMLDLFHRGRKEGKVDLKYSDEFLI
                     LYFQAMVEGISKPEIYAKLLPYTAEWTEVLIKGIAPKKK"
     gene            60620..61501
                     /locus_tag="SCJ86_000051"
     CDS             60620..61501
                     /locus_tag="SCJ86_000051"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310716.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEG1167648.1"
                     /translation="MAEIVKVTHLKKSFGRFEALKDVSFTVHSGEVVGFIGPNGSGKS
                     TTIRTLLGIIKKDGGTATIFGQDVWEDSLSIHERLSYVPGDISLWGNLTGGAIIDLFM
                     KLHGKGSAQKKDELIQRFSLDPKKKAKNYSKGNRQKVGLIAALAVDSDLYLFDEPTSG
                     LDPLMETIFQEEVEKLKEQGKSILLSSHILSEVERLADKVVIIREGTVVEEGTLDELR
                     HLTRSTITIETEAENSHLAQLNGVHDFVQKGHLAQFAADNEAFDVILAEASKLGVKKF
                     ESIPPTLEDLFMRHYEG"
     gene            61514..63124
                     /locus_tag="SCJ86_000052"
     CDS             61514..63124
                     /locus_tag="SCJ86_000052"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319543.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetronasin resistance protein"
                     /protein_id="HEG1167649.1"
                     /translation="MTDKFSRWSTLFSQNLSRDWKKILIWLVGVTAFSGGFVPAFEEL
                     TSGQGLAGLYETLQNPAMIAMVGPTPASNAAEYTLGAMYANEMLLFCSLFAAVISVLH
                     VVSQTRKKEELGLIELIRSFRVGRQANSLAVIAESILINLLLAISISCTMIFFQADSI
                     TAEGAWLFGLSVGSMGILGSAVALFAAQLMPSSASSNGLSLAFLGGLYLLRAITDVAD
                     SSLSMWNPLGWSYLTYPFTTNNWIPLLYLFLFSLILIISSFVLEEHRDLNTGYIPERE
                     GKPYAKRSLLSLRGLLFYLNKGMTISWLITFGLMGAAYGSIYGNMQQFLESNDLMKQM
                     FIQSGVSIEKNFTSTIMVVLICLAAILPIASINRLFTEETHGRMNQLFATKISRNQLY
                     WTNMLLAILFAILAILISSGALGLSALASMKDAKDMDLLMFLKAGFNFLPAVLVFIGI
                     SGFLFGWLPRMGKLAYIYLAYSFALNYFGGILDLPEWFSKTAVPSWVPRMPNDSFDLS
                     VFLTLSCISLALFVLGSIGYRRRDLLEG"
     gene            complement(63201..63860)
                     /locus_tag="SCJ86_000053"
     CDS             complement(63201..63860)
                     /locus_tag="SCJ86_000053"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289290.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Crp/Fnr family transcriptional regulator"
                     /protein_id="HEG1167650.1"
                     /translation="MSHDHLCVSFVPLFNHLVQQDQEKVHALVKHHYVEKGEQILAPN
                     GEPQLVIVASGSMKVYQLSASGKEQLLRVIEPGGYEGESQLLGVPNESLFGEALEKTE
                     ICILRQKDFSELLLHYPALSLKLLAINVEKLTKVQQQTTFLMMEKVEERVAVYLLDLA
                     KATGKDHFFLPMKMKELAAFIGTTPETLSRKFKLLEENGYVKRKNREVWIIDKEQLED
                     L"
     gene            complement(64143..64808)
                     /locus_tag="SCJ86_000054"
     CDS             complement(64143..64808)
                     /locus_tag="SCJ86_000054"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-6-phosphate 1-dehydrogenase family
                     protein"
                     /protein_id="HEG1167651.1"
                     /translation="MELGKSDYQLFDRPIYAFKQLKESHPTDKIEQIKKEYKEHWQKW
                     KEIQLQTAALLPDMYGMSKPKIESWTNGWNLRSHFWSAYRSESRQDENACLAVLLNQK
                     QYQIYLMYQHYKSEERYGSIAAYNQLLAILKEWSKTVDIKDYYIWPQPEHELDDHLAL
                     SDYLSDTKKQEELKKAMRDRTFQMGKLFFYPQEIEHVEQITAETLQELAPLYQKLGAE
                     KFQ"
     gene            complement(64955..65560)
                     /gene="dhaL"
                     /locus_tag="SCJ86_000055"
     CDS             complement(64955..65560)
                     /gene="dhaL"
                     /locus_tag="SCJ86_000055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319542.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroxyacetone kinase subunit L"
                     /protein_id="HEG1167652.1"
                     /translation="MKLTVQEIQEWLTQFAEAINQNKQYLSDLDTPIGDGDHGNNMGR
                     GVSAYEEAFQADHPETISDTFKVFSMAMISKVGGASGPLYGSAFMNMMKATKEVDSID
                     SQEKLGEVIEQGTAGIQSRGKAEADDKTMLDVWLPVTEALKAGNLTKEIIDQAKEHTK
                     VLVAKKGRASYLGERAIGHIDPGAASSAILFSTLLDVINQP"
     gene            complement(65588..66598)
                     /gene="dhaK"
                     /locus_tag="SCJ86_000056"
     CDS             complement(65588..66598)
                     /gene="dhaK"
                     /locus_tag="SCJ86_000056"
                     /EC_number="2.7.1.121"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289294.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroxyacetone kinase subunit DhaK"
                     /protein_id="HEG1167653.1"
                     /translation="MPERKDGNKLKKIMNDPSNIVEEMLEGLVKSYPELVHRVESSRV
                     VAKNQKAEQVGLVSGGGSGHEPSHAGFVGEGMLSAAVLGDVFTSPTPDQIQTAIKEAD
                     SGKGVLLIVKNYTGDALNFDMAKELAAMDDIEVESVIVDDDIAVENSTYTAGKRGVAG
                     TVLVHKIVGDAARNGASLAELKELGEKVVQATKTIGLALRAATVPEVGKPGFELGEDE
                     IEYGVGIHGEPGYRREKMQPSKVLAKELVTKILDDYSNLPKEAGVLVNGMGGTPLMEQ
                     FVFMNDVLALLEERGVNVVFRKVGNFMTSLDMQGLSLTLIDLTETQWKDSLESNVQTI
                     SW"
     gene            complement(66573..67019)
                     /gene="dhaM"
                     /locus_tag="SCJ86_000057"
     CDS             complement(66573..67019)
                     /gene="dhaM"
                     /locus_tag="SCJ86_000057"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319694.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS-dependent dihydroxyacetone kinase
                     phosphotransferase subunit DhaM"
                     /protein_id="HEG1167654.1"
                     /translation="MKAKPIVSNYVKERKILVKKSILLISHSQKVTDGIKEMIEQMQK
                     SEEVQIFSLGGINGEIGSDPMKIVEAVNESSDAASYFVFADIGSAVLNSELAKDMLEE
                     EQQQRYHLVDAPLVEGAFAAAITAGITDDTQQILTEAQNAGKKGWE"
     gene            67122..67442
                     /locus_tag="SCJ86_000058"
     CDS             67122..67442
                     /locus_tag="SCJ86_000058"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heavy metal-binding domain-containing protein"
                     /protein_id="HEG1167655.1"
                     /translation="MITTTTNSVEGKAVVAYKGIVFGEVITGIHAFKDLEAGLRNIFG
                     GRSKSYENELMGAREEALAEMAERAKALGADAIIGVKMDYEVLGSDNGMLMVTCSGTA
                     VSTR"
     gene            complement(67554..68423)
                     /locus_tag="SCJ86_000059"
     CDS             complement(67554..68423)
                     /locus_tag="SCJ86_000059"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015370988.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LPXTG cell wall anchor domain-containing
                     protein"
                     /protein_id="HEG1167656.1"
                     /translation="MGKVMKKRMKPLIWPIAVSSIFLLGQTAFAETSEPAQTEPSTTI
                     SSSGAENGASETEMTSNVPTDNSGETISSSEDTSINSSTSSDQIDHLQDLENKRKELE
                     EALEAAKNKLKEDQAAIDQDLADLKNKVGNDRAQLEKKLAEAKAKAEAVQTDLDGKIQ
                     QAKEFNQQLNQVAQTASGKLSQTQADITAASQAIHGKISEAQTVANDFEAKKADLAAS
                     FQRIKDILHSAGEKSVTSGSTTETVQQTTEPSKSTSQTLPKTNEKTASVSYSLAGAGV
                     LVAAAYGIIRKKK"
     gene            complement(68519..69822)
                     /locus_tag="SCJ86_000060"
                     /pseudo
     CDS             complement(68519..69822)
                     /locus_tag="SCJ86_000060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319539.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
     gene            complement(69819..70328)
                     /locus_tag="SCJ86_000061"
     CDS             complement(69819..70328)
                     /locus_tag="SCJ86_000061"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345422.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEG1167657.1"
                     /translation="MKKVTREEVASILVKDKYFSKGNYWLKIWQTLVALLGWMIVVLP
                     FIWVFFPLTRPERAKDFSLYAFLSEKKMLYFLIGFFVLIFFSFLITSFVLTRWNNRNL
                     YKKVNKSFEYEDEKLKKRQELLEEVYTERFGTKEERETVRFYSVSEEKNLDTTFIADL
                     YKENGVELK"
     gene            complement(70503..72638)
                     /locus_tag="SCJ86_000062"
     CDS             complement(70503..72638)
                     /locus_tag="SCJ86_000062"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HEG1167658.1"
                     /translation="MVNTQKQLEEAYLKNVYQKLIDKKNELRESMDTAKIEGNQSLKE
                     MAGELRLNFDSYLDNLDTFSMIEMKNREIDQMNIKQKNAENQLAKIERLLKSPYFGKV
                     VVDFLDNEPKESFYIGTANFTDDADENLVYDWRSPIAELFYNNMIGPSSYTVRQNKID
                     TVIKERRQFLLERDRLVKFFDTSVAIQDDVLLEVLGQDETNEMKAITATIQSEQNTII
                     RDVKSQNILVNGVAGSGKTSAIMQRIAYLLYLYRDTMTSDDLLILSPNHRFIDYISNV
                     LPSLGERNPLNLTIIQLVSQLSAEEIEGEEAYFKRITGENVSEQTERLRSKKFIDNLK
                     QSDPLFLDHPNFIRGLTKNGKTVLSKKTIEKIYEKVPAHPKLIDRLQATKKALMSEWK
                     NHLLKQAKSPAVQNQVLSLTEDRQLELFGKLISDDSEQSIVAYARKLLQKKYRKITRQ
                     IEEMAWVDEHQLFERIYEKRYGSAYAWQPTRTVDEAVIILAIRHLLVEKVNVPAFRYL
                     LIDEVQDYTLAQLGLLIELFPKTHFTLVGDENQAIFNSSTTFADIMRCFDDYHLPIHR
                     YDLRNSYRSSGAITELFKTYAVDQEKIDIVSIRPQGKEPEYRSFRSSEELLKILAEIL
                     VSLKGEKAAVITQTEEETQTLQESIKKNSYGLENCQIIPLSLAKGLEFDHVILYPFEN
                     DGDEQRRRRQMYTAISRGMKSIVVLERAT"
     gene            complement(72721..74376)
                     /locus_tag="SCJ86_000063"
     CDS             complement(72721..74376)
                     /locus_tag="SCJ86_000063"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HEG1167659.1"
                     /translation="MKIVIVGGVAGGMSAATRLRRLMEDAEIVVFEKGPYVSFANCGL
                     PYYLSGEISERENLLVQTPESLSARFCLDVRPNHEVTAIFPENKTVEVVHEGQKHIEQ
                     YDALVLSPGAKPVVPSIPGITEADNVFSIRNVPDIDKVMHALEKQPKRAVIVGAGFIG
                     LEMAENLKRRGLEVMVIEQAPHILPTLDEEMAAFIEKELSHQGVEVITSHAVAGFEDH
                     GKRLRLDDGRTIPADLVILSIGVRPDNQLAVTAGIELGIRGGILVDERYQTNIPDIYA
                     VGDAIVVKQQITGKDALISLASPANRQGRQVADTISGISRRNQGGIGTAIIRTFGMTA
                     ASTGLSERTAKENELSFEVIHVSGKDHASYYPEATDILLKLIFHPETGEIYGAQGVGA
                     KGVDKRIDILATAIKGHLTIFDLPELELTYAPPFGSAKDPVNMLGYAAMNIAEGLSET
                     VQWHELPTELAKGKILLDVRTAEELEKGKFKEAKHIPLNELRDRLNELDSQQEYIVSC
                     HSGLRSYLAERILKQSGYHVKNLDGAFSLYQTVRPEELIYPNK"
     gene            complement(74378..74692)
                     /locus_tag="SCJ86_000064"
     CDS             complement(74378..74692)
                     /locus_tag="SCJ86_000064"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010730940.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhodanese-like domain-containing protein"
                     /protein_id="HEG1167660.1"
                     /translation="MNLLFQSIKSITVPELKEKLLENPALLDVRTPAEYRGGHIKGAK
                     NVPLQSINRYDGDKEKTVYVICQSGMRSKQAAKELKKSGYDVVNVRGGMNQWFDRTVG
                     GK"
     gene            74862..75122
                     /locus_tag="SCJ86_000065"
     CDS             74862..75122
                     /locus_tag="SCJ86_000065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292566.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal-sensitive transcriptional regulator"
                     /protein_id="HEG1167661.1"
                     /translation="MEIDNKNILNRLKRTEGQIRGIQKMIEDEKECMDVITQLSAVRS
                     SIDRVMGMIVADNLKNCFEKPDSNPEEQAAKLEQAIKMIIKK"
     gene            75136..75450
                     /locus_tag="SCJ86_000066"
     CDS             75136..75450
                     /locus_tag="SCJ86_000066"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373590.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DsrE family protein"
                     /protein_id="HEG1167662.1"
                     /translation="MKVVFHIDELEKWSETGKNVKNLIKASPETTIVVSVNGIAITGY
                     LDSANAEFLDLQGVVFHACANAMRANHISESSLPEQVIVVPAGVLDLVELQSQGYAYI
                     KP"
     gene            complement(76180..76734)
                     /locus_tag="SCJ86_000067"
     CDS             complement(76180..76734)
                     /locus_tag="SCJ86_000067"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328737.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-alcohol phosphatidyltransferase family
                     protein"
                     /protein_id="HEG1167663.1"
                     /translation="MKLSTDWRKEIQTIPNLLSIFRIFLLPIYLYFVLRQSFYIAGAV
                     IVVSGLSDYLDGVIARRYNQVTDLGKVLDPFADKLTQLFLILSMAWYRPWLWLLFGLF
                     LIKEGFMFVAGLIGLSKNIKLSGAKWYGKVATAVIYVGMILLLLFPELPTLWVRVIFA
                     VITYGLLQSFVLYAVEYRKMFQRK"
     gene            complement(76860..78788)
                     /locus_tag="SCJ86_000068"
     CDS             complement(76860..78788)
                     /locus_tag="SCJ86_000068"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336853.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease MutS2"
                     /protein_id="HEG1167664.1"
                     /translation="MDNEMISQLQFDRIKKEIQARAIGNYSKKRISDMTVSTNLQTVL
                     DRQEETREARLILESSQHVPFMGLPRIDALTEQVKKGLILQPTDLIEYADFLRSSRMI
                     TKFFDKNQYQTPLLFAYSKHLPDLINVEELIDQKIKNNKVSDDASRNLRKVRKQLQLI
                     EKEIQSKLLKFLRHPKNKEMIQEAMIVQKGEYYTIPIKASYKNKIDGTIIDESNKRTT
                     VFIEPTVISKLNEHYQLLKAEEISEEYQVLAALTGAIAENEEVIDLLIETITVLDIIF
                     ARAKFSREINGSTPRINKSEHIIIKQGRHPFLPEHAVPLDVEIGKDYRGLIITGANAG
                     GKTVVLKTVGLLTLMAMFGMQVPAKEGTELAVFDEIFVDVGDHQNLENALSTFSGHMQ
                     NIAAILKKIKRNTLVLLDEIGSGTEPNEGAALAIAIMESMYEQGALIIATTHYGEIKK
                     FAGDHEDFVPAAMAFDREALRPKYQLRVGETGESQALWIAHKMDMSMKLIHQAERYLA
                     EKAYRTQKKEFLPKTAVTNRKENKKERQLFAKGDRIFVNEYQKEALVYEDIGEDTIDV
                     YLDKKIIHVPRQRVRLVRSAEDLYPTGYDLDSLFIDYKTRKRQRDLERGSKKAHKVLV
                     KEMRKRQEERRVNDENSK"
     gene            complement(79094..79810)
                     /locus_tag="SCJ86_000069"
     CDS             complement(79094..79810)
                     /locus_tag="SCJ86_000069"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329636.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aquaporin family protein"
                     /protein_id="HEG1167665.1"
                     /translation="MNSDMTQIMGEFIGTLILVLLGDGVCAAVNLNKSKAQASGWIVI
                     AFGWGLAVTMAVYISGFMGPAHLNPAVSLAMAMTGAISWNLVVPFIIAQVLGAFAGAI
                     LVWLSYLPHWNATKDESAILGTFATGPAIRNYPANVITELIGTFVLVLGLLAFGQNEF
                     APGTNVFAVGGLILAIGLSLGGPTGYAINPARDFGPRLAHAVLPIANKGTSDWAYSWV
                     PIAGPMIGAIIAVGVYSLMV"
     gene            complement(79811..81634)
                     /gene="glpO"
                     /locus_tag="SCJ86_000070"
     CDS             complement(79811..81634)
                     /gene="glpO"
                     /locus_tag="SCJ86_000070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315971.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 glycerol-3-phosphate oxidase"
                     /protein_id="HEG1167666.1"
                     /translation="MFSNKTRQDSIQKMQQEELDLLIIGGGITGAGVAVQSAASGIKT
                     GLIEMQDFAEGTSSRSTKLVHGGIRYLKTFDVEVVADTVGERAVVQSIAPHIPKPDPM
                     LLPIYEGEGATTFNMFSVKVAMDLYDKLANVTGTKYENYTLTPEEVLEREPFLKKEGL
                     KGAGVYLDFRNNDARLVIDNIKKAAEDGAYLVSKMKAVGFLYDGDQIVGVKARDLLTD
                     EVIEIKAKLVINTSGPWVDKVRNLNFTRPVSPKMRPTKGIHLVVDAKKLPVPQPTYFD
                     TGKQDGRMVFAIPRENKTYFGTTDTDYQGDFTDPKVTQEDVDYLLDVINHRYPEANIT
                     LNDIEASWAGLRPLLIGNSGSDYNGGDNGSISDKSFNKVVDTVSEYKENKVSRAEVED
                     VLNHLENSRDEKAPSTISRGSSLEREPDGLLTLSGGKITDYRKMAEGALRLIRQLLKE
                     EYGIETKEIDSKKYQISGGNFDPTKLEETVTELAKEGVAAGLEEEDATYIADFYGTNA
                     RRIFELAKEMAPYPGLSLAESARLRYGLEEEMVLAPGDYLIRRTNHLLFERDQLDEIK
                     QPVIDAIAGYFEWTEEEKAQQTKRLEALIAESDLRELKGEK"
     gene            complement(81648..83144)
                     /gene="glpK"
                     /locus_tag="SCJ86_000071"
     CDS             complement(81648..83144)
                     /gene="glpK"
                     /locus_tag="SCJ86_000071"
                     /EC_number="2.7.1.30"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019722534.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol kinase GlpK"
                     /protein_id="HEG1167667.1"
                     /translation="MTESTYIMAIDQGTTSSRAIIFDKKGRHIGSSQKEFTQYFPQES
                     WVEHDANEIWNSVQSVIAGAFIESGIKPNQIAGIGITNQRETTVIWEKDTGRPIYHAV
                     VWQSRQSAGIADNLKNEGYQEFFHEKTGLVIDAYFSATKIRWILDHVEGAQERAEKGE
                     LMFGTIDSWLVWKLTDGQAHVTDYSNASRTMLFNIHQLDWDQEILDLLNIPRVMLPKV
                     TSNSEVYGYTQGYHFYGSEAPISGMAGDQQAALFGQMAFEPGMVKNTYGTGSFIVMNT
                     GEKPQLSKNNLLTTIGYGINGKVYYALEGSIFVAGSSIQWLRDGLQMLQKASDSEDAA
                     KRSTSEDEVYVVPAFVGLGAPYWDQAARGAMFGLTRGTTKEDIIKATLQSIAYQVRDI
                     IDTMQDDTGIKIPVLKVDGGAANNEYLMQFQTDILNVPIQRAENLETTALGAAFLAGL
                     AVGYWKDTDEIREFYEAGKLFEVQMEEERREKLYNGWKKAVKATQAFE"
     gene            complement(83280..84740)
                     /locus_tag="SCJ86_000072"
     CDS             complement(83280..84740)
                     /locus_tag="SCJ86_000072"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344622.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HEG1167668.1"
                     /translation="MKIEALLERKEQLQILILRNLVLQGGTASINGLREHVQLSKASF
                     DQYLEDIELIGRMMEKKVEVQRNEYQALLVLDEDVSLEKILLFLLQESLKFKMLVYLL
                     EHQQVSIVRLATAFNISESSVFRKIKELNQLLEEFGLQIKNGQLYGEELQIRYFYYEL
                     FQYIPEDQRPLFLQNTPEKRPFILGLDRVLETTFTASAEAQIACWLGITKKRLLNEKS
                     TYATLKEKKLLYQSDRLYQAIDPIIVMHLSRTAAELNVYETMMFYSFFVSFSIVDEEI
                     FYQYDLTRSKKLPTAVLDTYIRETMLWHYRPRRLKIKEEKAVGYQISQINNEWFFFVG
                     KIEVYERDRLLEQQQKMLGHSLTQLLAKLQETAVQQLPRKRAEDSELSYLMIQYANVL
                     LMIDFYIAKPVVIGIDLESLPIYRIAFQQYLIRELRGIGGIEIGSYEEGKEYDLVITF
                     CQRNKKQCEYYLSEFASPYDIIRLKRRIETLKKEKN"
     gene            complement(84730..86025)
                     /locus_tag="SCJ86_000073"
     CDS             complement(84730..86025)
                     /locus_tag="SCJ86_000073"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HEG1167669.1"
                     /translation="MNIVIVGGSFAGIHAAIYLRQLMPTSEIYLIEKQSKLGWIPSGF
                     NLILKGKVTSEKQLSWITKEELTDRYRIHVYTEKTVIGLKEKKVVLAGGKKLDFDRLI
                     LATGSNQKFRNISAESTLIHPVKKIQNVTALQQKIMNAKKIAIIGAGQAGIEIAEGLA
                     SRKKQIHLYESRKSILFRYLDPEMTEPLVKEMKNQGLSLFLEEQVVSLTENETAIVET
                     EKRKENYDLVLLANHSRPDNQMWEEQLTLNDDGTIWVDDYLQTSQKDVYAIGDAIQVT
                     FRPTNEKMYVSLVNNAVRTARNVSKTISGSQTKDQGTYRPVGNQWFGYFLGSVGLTEE
                     ESIFYPQKIETGYVDYKLTAINQSKVKLKFLFDSEKKILGAQLQSKKEMFHLLDQLTI
                     AVEEGWTFEQLEEHELFFQPEYRVPDMVWSKVANTAYED"
     gene            complement(86357..87034)
                     /locus_tag="SCJ86_000074"
     CDS             complement(86357..87034)
                     /locus_tag="SCJ86_000074"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002351802.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 glutamine amidotransferase
                     domain-containing protein"
                     /protein_id="HEG1167670.1"
                     /translation="MKVLIVLTNVEKYEKIERPTGLWLSELTHFYDVLVKNGIDADFV
                     SPKGGYVPLEPQSVLNMDEIDWAYYEDEDFRNRALGNTLRPDDVDANDYAAVYYAGGH
                     GTMWDFPEAIDLGKVASQIYRNEGLVSAVCHGVVGLLPVTDESGKSILAGRTVTGFSN
                     EEEEANGTTNEVPFLAEDALKEKGANYQSGAAFSEVVQIDGRLLTGQNPQSAHQLGEA
                     VVEALKN"
     gene            complement(87064..88155)
                     /locus_tag="SCJ86_000075"
     CDS             complement(87064..88155)
                     /locus_tag="SCJ86_000075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-dependent flavin oxidoreductase"
                     /protein_id="HEG1167671.1"
                     /translation="MSQFDQSITLPSGVQLKNRLMLSPMTTQLSFFNGVITEDEITYY
                     KQRSKGLGAVITGAANVQDIGKGWPGELSIAHDEMIPRLSELAKAIQGEGAKAIIQIF
                     HGGRMTSRATLSGEQPVSASAVAAKRPDAETPRAMSEEEIVETIQAFGKATRRAIESG
                     FDGIELHGANTYLIQQFFSPHSNRREDQWGGSLENRYRFIEELLTTVFSAVEQYAKKP
                     FIVGYRFSPEEYETPGIRFEDTIWLLERLRESKLDYLHVSLNMYDRIARSDKYNDKSI
                     LEVVHNTIQGKIPLVGVGGIRNREDVEQVLKYAELAAIGQQMIVDPDWAEKLLENRED
                     EFVTKPFEEAYKELYLPSPLYNFLKARYQ"
     gene            complement(88256..88690)
                     /locus_tag="SCJ86_000076"
     CDS             complement(88256..88690)
                     /locus_tag="SCJ86_000076"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315975.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HEG1167672.1"
                     /translation="MEKPIEKALDECLFFSVKKLDRMLNKLADEAFRRTGLAPTYGFI
                     LLILKEKDGIPQKDIAQMLYSAPSTIARFVEKLEYKGYVKTVSEGRLSLVYLTEQGRQ
                     FAKEVDGSWEELHQSYKAVLGNEASKELAQTMNDATDKLQKK"
     gene            complement(89274..90731)
                     /gene="ascB"
                     /locus_tag="SCJ86_000077"
     CDS             complement(89274..90731)
                     /gene="ascB"
                     /locus_tag="SCJ86_000077"
                     /EC_number="3.2.1.86"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001336277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-phospho-beta-glucosidase"
                     /protein_id="HEG1167673.1"
                     /translation="MKKFPEGFLWGGATAANQCEGAWDVDEKGLSVSDVYTFDSDLPK
                     EKWTDQWHMMTHQQVKAAQDPNSKYYYPKRHGNDFYHHFKEDIRLFAEMGFKCYRMSI
                     AWTRIFPHGDEETPNEAGLFFYDQVFDECLKYGIEPVVSLSHYEMPLYLVTEYGGWPN
                     RKLIQFYVRFAETVFKRYKNKVKYWMTFNEINCVKHHPFVSVGVIEENHPHLEQAKYQ
                     GAHHQFIASALATKACHEIIKGSQVGCMISYQLLVPYSCDPDDIQKTIEQQRTSLFFS
                     DVQARGYYPAYTQRMFEEKGVNLKIEVGDEEILAAYPVDFVSFSYYMSSAVSAHPENW
                     EGVQGNLITGGIKNPYLPSSEWGWQIDPKGLRIALNQLYDRYQKPLFIAENGLGAIDK
                     VEEDGTVQDDYRIDYLKQHIEQMKEAIKDGVDLFGYTSWGCIDIVSASTSQMSKRYGY
                     IYVDQDDEGNGTKKRLKKKSFDWYRQVIQSNGEIL"
     gene            complement(90728..92623)
                     /locus_tag="SCJ86_000078"
     CDS             complement(90728..92623)
                     /locus_tag="SCJ86_000078"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002352007.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucose transporter subunit IIA"
                     /protein_id="HEG1167674.1"
                     /translation="MEKPFSKNYTYLVRRIATALINKGEVKMDYSQLAKDIVRFVGGE
                     ENVSNVYHCATRLRFTLKDNKKADKEKVEQLEGVITVVEAGGMFQVVVGNAVNEVYDV
                     LSKQMKLEDDASSGKRGTEKKGILNSFIDMIAAVFAPTLGVLAGSGLIKGVLALCTSL
                     NLLTTESGTYIILNAAADAFFYFLPIFLAYTAAKKFNTDRFIAMVIAAALVYPTIVSA
                     YSDSITLRFLGMPVILARYTSTVIPAILAVWVLSYIEPKIRKSLHESIRNLLTPFICI
                     IVMVPITLLVVGPIADYASQLIAAGYLAVYNFSPVLSGAVIGGFWQVLVIFGLHWGLV
                     PVMTNNLSFYGRDTLGPACMTAVAAQAGAVLGVFLKTKNKKVKSLSLSAFITALFGIT
                     EPAVYGVTLKYKRPFYIACICGAIFGGVAGAAGAGALAVATRSILSFPIYIGEGFVWL
                     VASYFLAMISSCMLTFLFGYKDEIEEESSKDIVLSTPAAGEIIDLSEVNDPTFASGSL
                     GEGFAIIPTDGKIYSPVNGEVSTVFPTKHAIGVVSEEGAEILIHIGIDTVNLNGKYFQ
                     SAVSDGKKVRKGDLLMEVDLQELIKEGYDPTTMVIVTNSSRFKNIITSHKKNNQTRKL
                     ASLGGSI"
     gene            complement(92681..94456)
                     /locus_tag="SCJ86_000079"
     CDS             complement(92681..94456)
                     /locus_tag="SCJ86_000079"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320969.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH domain-containing protein"
                     /protein_id="HEG1167675.1"
                     /translation="MFITKRRCKQMDLNKRQLQILVVLRNKNHWITSEELAEALGTNK
                     KTIQTEIKNMLAIYEKKIIIQSNKRSGYFLESIESETKQQIIQEVTKHKIYSSMDFRA
                     STIVTYLLFQKGYVSMQKIADIFFLSKTAVSLQIKTILRWIERNPKLELEVSSTKGLK
                     IIAEENSKRIFLSLVGTETVIQHARLPQQISTIFSNIMPTVQKTLKEVLISYNYIVSG
                     EDFIRFSRYLVLTILRREELLEKTNRYTLFIPMVETLTKKLSSELPYSFSNQEKQAIE
                     NRLLELNYLMINDEKNKEIEKNLRAFEQTIIRFLDLPVSLLFHETEFLLTHIKQMKTR
                     MDAGHNTMNHFVQKIISRYPLEIYLIRRFFPIHFQLRPNLAESAYFVLYLAEALNPFR
                     KQGEILIVSNQPISILNTLKKQIQQSMNMWIKECRIEPVYLFKNQSNKITEYDAILTT
                     EQELIFEWDDLIYLPSVLDEFESWEIYQILREKLTIVEEKKKTKLLDHYYKEENQIKI
                     HKKIDSIQQLISPSSDLVMLPLNAETLLCCLIKSQVETKITEYQLEIPLIHQQRKIKR
                     VFFVSFDYKRNDILVFFQAVSDLLG"
     gene            complement(94506..96002)
                     /locus_tag="SCJ86_000080"
     CDS             complement(94506..96002)
                     /locus_tag="SCJ86_000080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628189.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 1 protein"
                     /protein_id="HEG1167676.1"
                     /translation="MDQEIFPENFLWGGAVAANQCEGAWLEDGKLPNVTDTLIGIMNQ
                     HPSIQWNEEKKIWEIALDESLHYLSHEAIDFYHRFEEDIRLLKELGLKAFRTSISWAR
                     IFPRGDEQKPNEAGLVFYDRLINTLNRYDIEPVITLSHYETPLALVGEYGGWQNRKLI
                     DFFEFYAQTVFERYQGKVKYWMTFNEINNAFRMPYAAAGLVTFPARDKLEPIATLTKK
                     VIYQACHHMFLANARATQLLKKIDPNAKMGIMCSFSALATYAYDCDPENVFGSLQFKR
                     NSWFFSDVMCRGHYPAYIYRTWKEEDCAPVMLDGDEQCLQANTADYIAFSYYRSAVYK
                     KEAEMRVDTGGANGFDNPFLKEKSPEPWSWPIDPLGFRYVMNELTDRYELPLFIVENG
                     IGLDEAPDELNRIHDPKRCEYLKIHLQEIAEAIKDGCQIIGYLWWGPIDIVSAGTGEM
                     RKRYGFIYVDRQNDGSGSFRRLKKDSFEYYKQIIQSNGKNLEYRSFFK"
     gene            complement(96196..97818)
                     /locus_tag="SCJ86_000081"
     CDS             complement(96196..97818)
                     /locus_tag="SCJ86_000081"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000415199.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-glucosidase"
                     /protein_id="HEG1167677.1"
                     /translation="MEQKWWHHSVVYQIYPRSFQDSNQDGIGDLRGIISRLDYLAYLG
                     IDAIWLSPVYESPNDDNGYDISNYQAIMAEFGTMEEMEELIEEAKKRNIRIIMDLVVN
                     HTSDEHPWFIEARKNKENPYRDYYIWRDPVNGKEPNRLRSIFSGSAWQLDEKTGQYFL
                     HLYSKRQPDLNWKNKQVRQEIYQMMNFWIDKGIGGFRMDVIDMIGKEPDKEITSNGPK
                     LHEYLQEMNQATFGDKNLMTVGETWGATTEIAKLYSNPKRNELSMVFQFEHIGLDQQE
                     GKDKWDLKPLSISELKKVLSKWQTSLGDEGWNSLFWNNHDLPRIVSRWGNDDVYRKES
                     AKMFAILLHMMKGTPYIYQGEELGMTNCPIDDIAEAKDIETINMYNERISSGFTKEEI
                     LESINAKGRDNARTPMQWNAREHAGFTTGIPWLRVNPNYKEINAEEALADQDSVFYMY
                     KKLIDLRRKHDIIVWGDYELVEKTPEHVFAYSRIYEDQQWLVICNMSSEKQTFTVPRE
                     ALRVIVSNYPLKQLPDGKVDLRPYEAFVLEVK"
     gene            complement(98267..99580)
                     /locus_tag="SCJ86_000082"
     CDS             complement(98267..99580)
                     /locus_tag="SCJ86_000082"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631714.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NCS2 family permease"
                     /protein_id="HEG1167678.1"
                     /translation="MKEKILSYFEIEKLNTTVKREVLAGFTTFISMAYILFVNPTVLG
                     ASGMDEGAVFTATALASAIGCVLMGVLARYPIGTAPALGINAFFAYSVCLGMGIPWET
                     ALAGVFVASLIFILITIFKLREMIIDAIPADLKYAISGGIGLFIAFLGLSEGGIIVSN
                     ESTLVGLGSLNVGSTWLTIFGLVVTAILLVRRVPGGIFIGIAATTILGLVTGLIPMPS
                     EIVAAAPSLKPTFLVALKHVGDINSLQMWVVVLTFLLVTFFDTAGTLVGLANQAGFMK
                     DNKMPRVGKALASDSTAMLAGSLFGTSPVGAFVESSAGIAVGGRSGITAITTGIFFLL
                     GLFFSPLLSVVTSQVTAPALIMVGVLMAQSLRQIEWGNLEIAIPSFLILIGMPLTYSI
                     SDGIALGFIFYPITMLAAKRGKEVSPIMYGLFFVFVGFMWILNVK"
     gene            complement(99825..100049)
                     /locus_tag="SCJ86_000083"
     CDS             complement(99825..100049)
                     /locus_tag="SCJ86_000083"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338567.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEG1167679.1"
                     /translation="MDLMGRLEGYLKYRRAKHSEKLRAKNLKSGKKKTKGWGKMVDTG
                     FGGLNNLNPVDTKEFIKEEQKRNGKIKSHY"
     gene            complement(100069..100491)
                     /locus_tag="SCJ86_000084"
     CDS             complement(100069..100491)
                     /locus_tag="SCJ86_000084"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glyoxalase"
                     /protein_id="HEG1167680.1"
                     /translation="MESVYRQERKSNRKKGQMMIQKTRVMLYVNDVEMICRFFVEKIG
                     AEISETIELPEEFKSIVLSISKELELGVFPKVFVQKFSPEVLGPPPSLVFFTDEFETI
                     YENMEEPGEITDNNGILTFNFSDPEGNYFVIGKAESSD"
     gene            complement(100635..101345)
                     /locus_tag="SCJ86_000085"
     CDS             complement(100635..101345)
                     /locus_tag="SCJ86_000085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292544.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEG1167681.1"
                     /translation="MKKVIVDTNFWEVFPEATIEILSVNGIDNHVTKESEETYHQLLN
                     EAAKEARNYLTEETFSQNEVIAQWRQAFTTFKTKKGARSSIEALLKRANQEREFFPIN
                     PLVDIYNSISLSFGVPCGGEDIEKIAGNLHLGKAQGGESFLPLGADSDAPALPEEIIY
                     YDEQGAICRCLNWREAQRTMLTEETQAAVLVIESINAEQAARGAQAIEALKEKIDQTF
                     GVTSVRHTLTKDESEAEL"
     gene            complement(101363..101860)
                     /locus_tag="SCJ86_000086"
     CDS             complement(101363..101860)
                     /locus_tag="SCJ86_000086"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321138.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEG1167682.1"
                     /translation="MTPSDVDLLQQISIETFTDTFGKDNSPEHLADYLTQAYALEKLT
                     EELKDKETDFFLVSIDQEPAGYLKLNTGTSQSEEMADHFLEIERIYVRKKFKHQGIGT
                     FLLDTAIEKAKEKQKTAIWLGVWEHNDVALAFYRKKGFIKYSEHVFYMGDDPQTDYLF
                     IKQLF"
     gene            complement(101897..102349)
                     /locus_tag="SCJ86_000087"
     CDS             complement(101897..102349)
                     /locus_tag="SCJ86_000087"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300731.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HEG1167683.1"
                     /translation="MAEILRDIGMIARALDSISNIEFKEVDLTRGQYLYLVRICENPG
                     IIQEKLAEMIKVDRTTTARAIKKLESNGMIERLEDKENKKIKKLYPTKKGAEIYPFII
                     RENNYSNAVALNGLSDEEAKQLEYLLNKVCKNVSEDWNFVKKGNKRIY"
     gene            complement(102484..103089)
                     /locus_tag="SCJ86_000088"
     CDS             complement(102484..103089)
                     /locus_tag="SCJ86_000088"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326433.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX hydrolase"
                     /protein_id="HEG1167684.1"
                     /translation="MNKKLAQYKRLLALSQAGLYYGKDVFDKERFEEIRTISLELISE
                     IGKETFEEIKALTTIGEGHPTPKVDVRAYIKKDGKVLLIEDKRTKEWSLPGGFAEIGL
                     SPEENVRKEVYEETGLTVETTQLRAVFDTNKQKDIPQLFQYYKLVFACAIHGEEAKFI
                     ENNETSNMGFFSIEELPKLSEKRTTKKQLLILENKNQIYCD"
     gene            complement(103539..104159)
                     /locus_tag="SCJ86_000089"
     CDS             complement(103539..104159)
                     /locus_tag="SCJ86_000089"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HEG1167685.1"
                     /translation="MKKYFILLAGSPGTGKTYLMNKLRERFPDMYAITLDEIKEYYAE
                     SIGFNNLEERAEQERTKVYPFYYKALDLYMEAGKKVIVSEYPFSDKQKARLQELADRY
                     GYQVITIRLTADFEVLCQRRYKRDRESDRHLSYIMDHYHYGDVLEDRTKGTNHITKEA
                     FKKIIADRKYDKFQLGTLYEFDVTDYTQVDYTPLLDKLEKEINDNK"
     gene            complement(104161..105030)
                     /locus_tag="SCJ86_000090"
     CDS             complement(104161..105030)
                     /locus_tag="SCJ86_000090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341038.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class II fructose-bisphosphate aldolase family
                     protein"
                     /protein_id="HEG1167686.1"
                     /translation="MYTTLKEVTKTAEELNFTVGAFNTHNLEMLPAMLGAAKEMGSPI
                     IIQTSVDTAKYIGMHVLVNVMTAIAEEEMVDAVLHLDHARDFDDIKEAIDSGYTSVMY
                     DGSHLPFKENILKTKAVVEYAHAHGVSVEGELGTIGGTEEGIHVAENDKVYTRPEDAV
                     KFVEATGVDALAIAIGTNHGQFKSKTEVNIPLLKEIDAVVDVPLVIHGGTGVKEEDYP
                     ELINNGIRKFNVGTELLVNWTKVAKETFSETEINKSLRHNVIPANEAVKEIVKHKIGL
                     FMNLESPINLGNK"
     gene            complement(105053..105460)
                     /locus_tag="SCJ86_000091"
     CDS             complement(105053..105460)
                     /locus_tag="SCJ86_000091"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314026.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HEG1167687.1"
                     /translation="MFGFFKKKEKEPKKVLAKEEKALSPEATEKIQSEITQLKQEIST
                     TQDKHKLAKLYEQVGLKFSELYVNDQAIQYLEKSLENKQTIGDGYKKLMSLYNQKRAD
                     AARAGDDQGIDYYMGKMDEMRQIAKQVTIKGNK"
     gene            complement(105479..106768)
                     /locus_tag="SCJ86_000092"
     CDS             complement(105479..106768)
                     /locus_tag="SCJ86_000092"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294703.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HEG1167688.1"
                     /translation="MGVINFIIENILTQASITIALIAMLGLLLQKKSAGQVISGTLKT
                     LLGFQVLSAGSSIIVGSLTYFGKIFTEGFHMQGIIPSIESINGQAMNDLGLGRDIALT
                     FLAIFVFNIILARFTKWKYIFLTGQAILWMATMTTVFGYFAGLRGIVLILVGGFIGAC
                     FAIAMPAVAQPIIRKITGSNDIALGHFCTIGYLFEAGVAKLFGEKGENKKSIEDIKLP
                     THFEFLQDTYLSVMVVMVPLYIITVLFAGEPFASELSGDQNYIMFAFLQAIQFVVGVY
                     VLLAGVRLLLGEIVPAFRGIAMKLVPDAIPALDCPVFFPYSPNAVILGFITTTIGTII
                     AMFTLPMFGLAMILPGMLTNFFAGGTAGIFGNAVGGRRGAIIGGIAHGFFITLLPALL
                     VTIFNSMGFINATATDVDTVAAALLYAWILSPVLKAF"
     gene            complement(106795..107097)
                     /locus_tag="SCJ86_000093"
     CDS             complement(106795..107097)
                     /locus_tag="SCJ86_000093"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335168.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HEG1167689.1"
                     /translation="MNKLNILFVCGAGLGSSFAAQMSAEDVLNKLGVEAKLDHCDISS
                     AVSMKPDVIITAENFRSQFEKFAVDPKTAMIYLKNIVSKNEIEEKITPVLQDKGVI"
     gene            complement(107122..109215)
                     /locus_tag="SCJ86_000094"
     CDS             complement(107122..109215)
                     /locus_tag="SCJ86_000094"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BglG family transcription antiterminator"
                     /protein_id="HEG1167690.1"
                     /translation="MKTRTIEILHRIMTAQYPVKAKHLASEFNVSQRTIRQEVLEANI
                     WLSSKKLPEIRTIRNKVFLLKLEKEKQARLETEIKDVKDGFLNRRERIFDLVLAVAYS
                     RIPIHLNKKEEEFQISKSTLDEDMRRLRATLVSYGIEIVSYGKQGLVYKGSERSIRTM
                     IYDFINQNLGMTDFFMNQDAQLTPAQKIFLHYFPLVEIKKIQEIYSNKLLKQEDDIYK
                     KQILLFTLIWIQRIRKHEFISAINWKNTEIKDSSFSAFIDHMIEEFQLDNVPQVERNY
                     LRFTIETFNARDISNSIEWVQAQLLTIQLIQFVENETHVPFHLKEEALFENLYKHMAA
                     LIVRIKNHIQVMNPLKENIRTNYFPIYRAVTHFVPTIEEVIGGKILEDEIAFLVIHFS
                     TIASVIKRDLNYVYKSVVVCNHGMATGNLLAENLKEKFPQIEITAVLSSKEIELVDKL
                     DVNLVFSTFQLNYRNKPLLVVDPILTETNILVIEDFLKRNEELQRLEPNSHESTELFT
                     QIIDVMKESGGRVDRIIYGKLEKLFETNNLEINKREIQPMLKDILTDSNIMIKEKATT
                     WEESIELAARPLVKENIVEERYVEAMIRAVNDYGPYIVIGKHMALAHARPEDGVNKLG
                     VSVATIEQPIDFGNPEMDPVKIIFCLAAVDSYSHLNIMKELIELINNEEKLDRLIECT
                     NIEDFKQLLFSVVEK"
     gene            complement(109704..110468)
                     /locus_tag="SCJ86_000095"
     CDS             complement(109704..110468)
                     /locus_tag="SCJ86_000095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853117.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIM44-like domain-containing protein"
                     /protein_id="HEG1167691.1"
                     /translation="MKKINYLAIILLVGLCLFALPTQGFAVAGGHGGGTSSGRSSGRG
                     SFGGRSYGGSYFGSRSYGRDSYGYYGPGTGPSSYEQTGFLALAVLSVGGVSAFSIVKN
                     KRQKKRLKAEILSRMSGTAKEKKAQFLEIQKIFMKIQSAWEEETPEKARSCYTDKLFA
                     EHQKVLQKNKADGVRNHTKKITIQEVGNYRQIHENSFAIRIDFRCCDYSVDRKDGRLI
                     SGSKTQRQYFSQIWYFDFSEKEKRWQADFIQPIMLD"
     gene            complement(110579..111448)
                     /gene="mdcB"
                     /locus_tag="SCJ86_000096"
     CDS             complement(110579..111448)
                     /gene="mdcB"
                     /locus_tag="SCJ86_000096"
                     /EC_number="2.4.2.52"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287541.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="triphosphoribosyl-dephospho-CoA synthase MdcB"
                     /protein_id="HEG1167692.1"
                     /translation="MINDERKKAVTQQLAALVEEALIAEVTLSPKPGLVDALDAGAHS
                     DMDYALFLKSAKTLTPFFEEMAQIAWHHAIDQELRERIAEIGRKAEKAMLIATNGINT
                     HKGAIWAMGLLISVIAQKISMEQPFSLSKILDDVSKLAAFPDTKYRHKKTTHGHRVKQ
                     KYGVNGAYEEAILGYPHIRLAIQAAKELDNDSYVQKQMHMLLVLMSSLDDTCVLHRSD
                     QKTLVEMQKLAHAASQSALPNRPFTALLHFCQENQISPGGSADLLSASLFMLKIEELW
                     TADTYICPMLLND"
     gene            complement(111473..112549)
                     /locus_tag="SCJ86_000097"
     CDS             complement(111473..112549)
                     /locus_tag="SCJ86_000097"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287542.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyltransferase"
                     /protein_id="HEG1167693.1"
                     /translation="MFENKKIKINVSDFQKVSAVTAVMMQTILSFALANSHSATNTAF
                     LGTFYVIVKYTAPMFIFAIVYNMVKTSQHLSYWEFLKEKFFELVVPYLLWTTAYLWLF
                     PAVQQQTPYTDTASFLLKYVTGDGAPHLWYTVMMLQIQLFMPFFVWLGYKVFANKKYV
                     WPVLIGSTILYVAWYLFYQTQVLTGPYHDSWYLLDRFVASFMIYGVAAFVYHEKVYQY
                     LDRVRYLFLPVALVIAFFSVRSLLAHPGDLSFANAPYLNTIQSLYSLVIIFAVFIGAS
                     KMIVNDSPKLPLFKWLSVYAYRTYLANVFVFQVLLLLFKDSWLQLPSGVMILVAYLMT
                     ASCAFALSWLLHIIWVAIKKGFSK"
     gene            complement(112573..113166)
                     /locus_tag="SCJ86_000098"
     CDS             complement(112573..113166)
                     /locus_tag="SCJ86_000098"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292526.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="malonate decarboxylase holo-ACP synthase"
                     /protein_id="HEG1167694.1"
                     /translation="MIQAHDIVLFDKHEIEGFTLPEWVVLTEKMFGTVRRASTDHAEI
                     LPIGLRGPARNQRWSTEISKHAVKQVIHPWDIIEQESYRQEKIMDYPVYQQFHAARNI
                     LSGCKWGIGGSLGFELSTGIPAVKETSDFDLLLYANSPIELPIQVIQSHPAFFEQFDT
                     QVVTRQGGFSLKEYLREPEKKILLKTVEGPKLTKELW"
     gene            complement(113170..114846)
                     /gene="mdcD"
                     /locus_tag="SCJ86_000099"
     CDS             complement(113170..114846)
                     /gene="mdcD"
                     /locus_tag="SCJ86_000099"
                     /EC_number="4.1.1.88"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336658.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biotin-independent malonate decarboxylase
                     subunit beta"
                     /protein_id="HEG1167695.1"
                     /translation="MKNSFVELNARERVNALLDNGRELIDPFDQIIAPSLPAQGIVPE
                     SDDGIVVSRGTINGKQAVVIAMEGKFQGGGIGEVSGAKIVAALEHVLEENKKGNEIFP
                     VIVLDTGGVRLQEANYGLLSISEIGNVIIALKQYVPVIGLIPGRVGSFGGMSITSALM
                     SYLIATKKARVGLNGPEVIEQEAGVREFDSSDKDLIWNTLGSRQRVESKIIDELVTDS
                     VEAIKAAVIAAMDERKDTHRSESSAFYLSLLDSLDLSKPMAIDDYNEAIANHQAKSID
                     LPEVAEGNEPAAASVGYDWFAALTGMKNPTSSISTVYYGKSSKFIEEAIVTSIVPDPK
                     NPMYRVRNGEVGLVEGFRMGQILDHVYEEDKDKKVKRPIIVVIDVPSQAYGYNEELIG
                     IHVALANSAAAYAKLRQAGHPVIGLIAGNAISGAFLAHGLQSSRLIALNNKEITVQAM
                     SKESAARVTKRSVAEIEAAAEKVPAIAYDINNYTKLGAIYHFLEDITDQAASEQNVSK
                     VIEAINEAIQDVRNTNDTMLTNRYTNETAKTFGRVETNKVRAKMDEEWDA"
     gene            complement(114836..115135)
                     /locus_tag="SCJ86_000100"
     CDS             complement(114836..115135)
                     /locus_tag="SCJ86_000100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016624640.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="malonate decarboxylase subunit delta"
                     /protein_id="HEG1167696.1"
                     /translation="MEQLAYKYPTKQPINKKIHVGVVGSGDLEILMFPTEKTESTVKI
                     CTGSDGFGEVWNNVLTRFFDRYPISADIVINDFGATPGVVYLRLTQAMEELSDEK"
     gene            complement(115237..116883)
                     /gene="mdcA"
                     /locus_tag="SCJ86_000101"
     CDS             complement(115237..116883)
                     /gene="mdcA"
                     /locus_tag="SCJ86_000101"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010748322.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="malonate decarboxylase subunit alpha"
                     /protein_id="HEG1167697.1"
                     /translation="MEKPNRNWASKREKKAQRLEKISNLLNGKYVETEKIVEVMEKLI
                     APGDKVVLEGDNQKQASFLSESLEKVDAEKVNDLHLIMSSISRPEHLNIFEKGIASKI
                     DFSYAGAQSLRVAQMIEDGTMKLGDIHTYLELYGRLFIDLIPNVVLVAADKADKDGNL
                     YTGFNTEETPTIIEAAAFKDGIVIVQVNEVVDSLPRVDIPGDWVDVVVKADKPYQLEA
                     LFTRDPQNITELQILMAMMAIKGIYAEHGVQSLNHGIGFNTAAIELLLPTYGESLGLK
                     GKIAKNWVLNPHPTMIPAIESGWVESIHSFGGEVGMEKYIAARPDVFFVGKDGTMRSN
                     RTLSQAAGQYAIDMFIGSTLQLDYEGNSSTVTKGRLSGFGGAPNMGHNPGGRRHSTPA
                     WQSLRDNDDPLGKGQKLVVQMVETYGSNKKPVFVEELDALAVQKQAGLANAPVMIYSE
                     DTTHIVTEEGIAYLYKAKTKEERQAAIAAIGGVTPLGMTSTKESLDSLRAAGIVKLPE
                     DLGIKRSEAKRSLLAAQTIDDLIEWSDGLYEPPMKFRTWS"
     gene            complement(116890..117801)
                     /locus_tag="SCJ86_000102"
     CDS             complement(116890..117801)
                     /locus_tag="SCJ86_000102"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325257.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ACP S-malonyltransferase"
                     /protein_id="HEG1167698.1"
                     /translation="MSTGFLFPGQGSQKLGMLADIPEMYMERLENVTGYRLVDKEENY
                     QDTVFIQLALLTKAVFYLDEMEKNGIQPDIAAGHSIGAFCAAVACRSLRFENAAELVY
                     HRAVLMKEAYPSGYAMGVVVGVTRSEAEEIVAKTFDPDHPVYLSNENCPMQHTLSGSI
                     EGLSVTLAEAKRHQAQTAKLLKVPVPSHCLLMNDTVKKFKPYMQSVHLSDARCLYIKN
                     TDGRATQDAEEIRKDLLENLAFPVQWNQMMDLAKELGMDLTIEFPPGNTLTKLIHTKF
                     GVDKGIRTINLDQHGIDDATFLYQKWR"
     gene            117921..118778
                     /locus_tag="SCJ86_000103"
     CDS             117921..118778
                     /locus_tag="SCJ86_000103"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HEG1167699.1"
                     /translation="MKLLHLQYFKKVFETKNVTQAAKELFISQPALSRAIKHLENELG
                     VPLFYHSGRNIETTVYAEEFYPYAVKTLDTLEEGIKLLSSVNEKIVTSVILYLEVASV
                     SIPNLVRIFMEKHPDIQLTIMQHHLPDDITDPVLYITSEMKPGMTNIPIMKEPVYVAI
                     PKKHPLAKKEHLQLEDIQRTPILMLSKSNAFRHTLDAALEAKSIELTISSTTDDPATL
                     RSIVKQGLAVSFFPKVSWSYDKNDPFVLREIKDLPLTRTIYLSSSFTEDHPLIKMIAK
                     TMREFYLGK"
     gene            complement(118965..119327)
                     /locus_tag="SCJ86_000104"
     CDS             complement(118965..119327)
                     /locus_tag="SCJ86_000104"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MmcQ/YjbR family DNA-binding protein"
                     /protein_id="HEG1167700.1"
                     /translation="MLTKKELIDHINETYNLSPEYIFKKFPDYCVFRHQKSKKWFGVL
                     MSVPEEKIYDDASDHRIINILDLKVDPELGGILRGKEGYFPAYHMNKEHWISIDLRKI
                     EKLDELADLIDGSFKSTQ"
     gene            119673..121112
                     /locus_tag="SCJ86_000105"
     CDS             119673..121112
                     /locus_tag="SCJ86_000105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331954.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HEG1167701.1"
                     /translation="MYSLMKKIITEKDIRRHVSLVEQLLNHTKITVNELAEIIGTTER
                     TIFSDLQSIRSQLPEGWDIISDQAGISLQNQQNLLTNDLWEIFFKQSVSVELLKNLLF
                     TKKVAVPDFLADHGLSYGTLKRHVTKINQRLASYDLQIDLTKYTACILGKERAIRTFY
                     HRLLIPFTHNNYFFEDYSIHESHYFQFLRNLSQTELAVETEEIFGTCWFFINTIRIKA
                     NCRLDSSIHTNSTLSSLYDSALKKLYLTEGIYLKDTELSFASFCFLESWNYNNNYGQE
                     TARCLHHSPFLEVLETFVEELASELSLDQLKKTSLTDNLALLILKYHESPILIEALDR
                     QYHRFLETYDQQYAHLYQQKENLLNDLKKEIEINEPNYFLQLLSLLIQQTIFSIKPNL
                     FNVYFIFQGEPSWKAFLQQELKDYLGKRVCFLPIELTDLSTISFEKTDILISNFPLDS
                     LDIPIVYISSIPTKNELNRLTELTFKTFL"
     gene            complement(121119..123671)
                     /locus_tag="SCJ86_000106"
     CDS             complement(121119..123671)
                     /locus_tag="SCJ86_000106"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294713.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD-IC family P-type ATPase"
                     /protein_id="HEG1167702.1"
                     /translation="MNWYRLTVEDVLKNYQTSTEGLSQEERQKRLTDQGYNEIKARQQ
                     TKRWKKIAKHFTDLLMIVLIIASFLKFASSEYIEGSIILFVVIVNGLVSYWQERKAEE
                     SLNGLKQLMGQEAIVLSNGMQEKIPAKELVKGDVIVLRAGDVVPADIRLIEAHDLLVE
                     ESILTGESEASEKSHAVLTEEESIGDQKNMAFSGTLVQSGSAVGVVVETGNTTEIGKI
                     NQALQSVEQQTTPLIKKINRLNKQIFQGILCLSLFLVIFTTFRYGMDWHILLSATIAL
                     VVSMVPEGLPAVLTMILSVGVHEMAKEKAIIKGLPSVETLGSMTVICSDKTGTLTKNE
                     MTVVDVAAKEEACVLSIMKNCQELKTKEEQKTENLSGNPTEVALIRHTETANLPLKKI
                     EAKIPFSSEYKYMATMHAEEKGAVIYVKGAPEVLFAKSTLSSAEQEAWSQTAAEFARK
                     GQRVLGFAYKKVDSKQELTHETLTQLTFAGIAGLIDPPKESAVKAVKECQQAGISVKM
                     ITGDHKDTAKAIAEQIGLKHTAKVLEGIDLDLMSDEELIQQVPIVDVFARTTPEHKLR
                     IVKALQKNGEIVGMTGDGVNDAPALKRSDVGIAMGIKGSEVSKQAADMVLGDDNFHTI
                     AKAVKEGRRILDNLQKTINFFLPTALAQGLILIWALMLNRPLPLSPVQILWVNMVTTI
                     TLSYALGFESPDPEIMKRPPRDPKQGILSGYHLFRIIYVSLLIMVPAYLLAMQFEGQS
                     LQQTILLQNIVLAQAVYMINCRELSKPSLNQGLLQNKALFLSLGILALLQGMVIFLPV
                     GQQLIGTVSLTMTQQLLIGANVILLFLVVEIEKGIMNKLFRKKEKTAREYSR"
     gene            complement(123785..125116)
                     /locus_tag="SCJ86_000107"
     CDS             complement(123785..125116)
                     /locus_tag="SCJ86_000107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HEG1167703.1"
                     /translation="MEKPAMKRTLTNRNIQLIALGGAIGTGLFLGSAKAIASAGPAVI
                     LVYLICGLAIYGLMRAMGDLFLANSTFNSISDFVDYYLGKKWAFFVNWTYWLCWLGAG
                     LAELTAIGLYIHFWFVHVPALISGGSALMILMAVNLVAVKWFGELESFFSIIKILGIF
                     LFILLGIGLSVSDFHTQSIGIKENFGQIDELLPFGFAGMVVSFPMVLFSFAGIEMIGL
                     TVGETSDPQKNLKKAINCLPWRILFFYIGTILSILFVVPWHCLDLKESPLVTMLQVIH
                     CQGGAAIVNLMILVASLSSANSAVYSTSRILYQTAKEKSLPHWFCALSKKGVPLHGIL
                     ITGMLFFAGLCLHFFVADSRLFFQLLAGMTTSCFLFVWSSILCAHVQFIKEKKRNTTQ
                     RYKDLALLVFFTSIAFSLLFESTTRFIPFLLVIWFVFLNFVYKRISNRNIS"
     gene            complement(125082..126560)
                     /locus_tag="SCJ86_000108"
     CDS             complement(125082..126560)
                     /locus_tag="SCJ86_000108"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302154.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HEG1167704.1"
                     /translation="MKDTGSSKQITTMTFIGMTCALVASVRNIPDVAATSWTMIFYML
                     VAVLLYAFPISLISGEFAGMFPQKGGPELWVSNALGKKWGFVVSWLLWVQMFPGMVMV
                     ASALAPLFGNIIDNVPLGLNSKFTLVVILVVYWIITLLNLKFDMAKIGGKVGVWLGLY
                     VPLTMMLLLGFAAWIKVGIVPTGTLGSFSWDKLIPDTTTASSFVYFAPIMFIFTGIEM
                     SSVYITRLENPVKTYIRGVFAALIFIFFVNILNALVVANVVPKGQMELNNIAQSISIY
                     CQILGLPHLIVNLFSLLVFIGVAVQLSAWASGPAKTVTESARKGAYPPKFNFWKTNQF
                     DVSKSVILTQSVIISIFALFYLLIPGVNQAFLMLVNSTTVIYCIVYVIMGIAVLRLRY
                     THAKLNRPFRIGKKEKKSNLGVWTVVIVLFAAIGFSVGLTMKAGTWINLIAVTAISVI
                     LFVVPLCIEKIKKPSWEQEVAMLLEKEKGELDGKTGNETDSN"
     gene            complement(126865..128700)
                     /gene="tdc"
                     /locus_tag="SCJ86_000109"
     CDS             complement(126865..128700)
                     /gene="tdc"
                     /locus_tag="SCJ86_000109"
                     /EC_number="4.1.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine decarboxylase"
                     /protein_id="HEG1167705.1"
                     /translation="MKDMDIKAVFIGDKAENGPVYKMLLNKMVDEHLGWRENYIPSDM
                     PAISEGDKLTPDYLATRDHMIEVLDEVSQRLRAGSIPWHSAGRYWGQMNAETLMPALL
                     AYNYAMLWNPNNVALESSMATSQMEAEVGQDFASLFNMTDGWGHIAADGSIANLEGLW
                     YARCIKSIPLAVKEVLPEKVKKMSEWELLNLSVEEILEMTESFTDEEMDEVKAASSRS
                     GKNIQRLGKWLVPQTKHYSWMKALDICGVGLDQMVAIPVQEDYRMDINALEKTIRELA
                     GQKIPILGVVAVVGTTEEGQVDSVDKIVQLRERLKDEGIYFYLHVDAAYGGYARSLFL
                     NEAGEFVPYASLAEFFEEHHVFHHCVTIDKEVYEGFRAISEADSVTIDPHKMGYVPYA
                     AGGIVIKHKNMRNIISYFAPYVFEKSVKAPDMLGAYILEGSKAGATAAAVWTAHRVLP
                     LNVTGYGQLIGASIEAAQRFREFLDHLTFTVKGKTIEVYPLNHPDFNMVNWVFKEQGC
                     TDLNAINELNEKMFDRSSYMDGDVYGERFITSHTTFTQEDYGDSPIRFVERMGLTKEE
                     WKKEQKITLLRAAIMTPYLNDDRIFNFYTKKIAKAMEKKLNEIIQ"
BASE COUNT        37757 a        26967 c        22549 g        41724 t
ORIGIN      
        1 ttttttgcct atcagtgcaa aagcatttcc tgtgcaattt cacgtccatc cattgtatct
       61 ggataaaatg ttggccaatt ctccatttct ttcagcaata cttcttgact ttcatttcct
      121 gcatataaat gaaaatgttc ggagtcagcc ggtgcaatcg tgtgatcact gaattgaata
      181 tatctaaaag cgccactttc cttatcttct gcttcgaata gataccttac gccacggttt
      241 cctgcctcgt aggtcaagat ttctttccct acataacgat aatttgaacg tttcgttgtt
      301 ccatccttaa acgtaaatgc catctctttc tcactgatct tgattgaagc aatatctgtt
      361 tgatagccct tcgtataata ttctttatat tcttgtgctg tcttatcctg attcaatttt
      421 tctttatatt caaaaacagg atcaaaagtc ccatcttcta catacgtatc tactgtttgc
      481 caagttcctg cccaatctga aagagagcga tcttgtacat cctcgtcttc aaaataaccg
      541 ttgtagacgc tcttttcttt tttgggggtc aaagtatctt ctagcgggtt ttctgtctct
      601 gtcgtttttt tcaacgcttc gaggtttgct tccatgatag aaagatagtt ttctccattt
      661 tctatttgtt cattcgtcaa tccttccaaa ggattcaata cttctaaaga tacacctgct
      721 tcgtttgcta acgttctggc aatactatct ttggcgtttt cttcaaaata aatatagttg
      781 attccatgtt ctttaacaaa gtgtttcagt tcagcaattc tcgccgcaga cggttcttca
      841 ctagaagaaa gaccagcaat cgatacttga ttcaatccat attctaacgc tagatagttg
      901 aatgcagcat gttgcgtgac aaaagatctt tgttttgctt gcttaaaggt ttcttgataa
      961 agctgatgga gtgcttgaag tttctcggta taggctgctg cattttttgt ccatacttcc
     1021 tgtttttctg ggtaagcttc aatcagctga tcacgtatgt ttgctacctg cttgattgct
     1081 aaacttggag ctagccatac atgtgggtct agctcatgtg tatggccttc ttccccgtgt
     1141 tcatggtcat gatcatgtcc ttcttcactt cccggaagta aaagcatacc ttctgtcgct
     1201 tttatcgtat ggacattgcc ttcttgtaat gttttttgga ttgcggggac ccatgtttcc
     1261 atgttttcat cattataaac aaagatatct gtatcttgga tctttgccat gtctttagca
     1321 gaaggttcat aatcgtggga atcagtccct gctggaatca ataactctac ttctccttca
     1381 tccccgacga ttgcttttgt aaaatcatac atgggataaa aggtcgtcat cacgctcaat
     1441 ttttctttac tgtcctcggc tttcccactt gccccgcaag cagttaacat acctgctaaa
     1501 accagcatgg ccaatatacc tatcaatcgt ttcatttatg acactcctct tctcatcttc
     1561 tttcatttga atgttccgat tcgcttttcg caaaccgtaa tagttacgtt ttagcaacag
     1621 gatttaattt atcatatggt taaaagaact gtcaagcgtt ttcttatttt aattgatgag
     1681 aaagtttata tgaatgattg acgatatttt tgcaatcaac ctctatcatt ttccttttat
     1741 acccgctttt gtctataatg aagttataga taaaggagga aaaagagtga aaaaagaaac
     1801 agatacaacg aatcgtttga tccgcttttt aggaggaaaa acctcttact atgttttagg
     1861 aatagttatc ttacttgcgc tgatcattta ttttttccat cagatctcgt ttatattcaa
     1921 gcctgtcgga atcattatcg ctactatctt gcccccatta ttgtttgcac tgatcttgta
     1981 ttatcttttg gaaccagtca tcaaacgttt gagcaagcac ctttcgagga acttgtcagt
     2041 cattatcgtt tatgtgatta ttttattttt attggggctt ggaagcatat ggcttattcc
     2101 ttttcttgaa gaacaggcaa agacattgat cagtagtttg ccagatttgt ttcaagattt
     2161 tcaagagagt ctccgacaat ttttggagaa aacacctttt gccgattcat ttaatcaatt
     2221 ttttgcttct attgacgacg tgacgtctga tatagctgga tttattggaa attattggga
     2281 aagtggcgca caacggatca gcaacatttt ttcaactgtt acagctattt ttattacttt
     2341 atttactggt ccaattgtcg cttttttcct tttgagagat cctcaaaaat tttatcagtc
     2401 agttttagcg attgtcccac cagcatttcg tacagatttt aaaaatttga caaagattgc
     2461 taatcaacaa ttgggttcat ttctaaaggg gcaaatcatt gcttcattta tccttggagc
     2521 agtctactgg gtttgtttct tactgatcgg attagaattt gctagtgttt tagctatttc
     2581 tgctgggttg ttatgtatca ttccatatat tgggccgttt atcgccttct ttccaggttt
     2641 gatcattgca ttccaagact ctacttttat ggctgtcaaa tttgtcattg tctggtttgc
     2701 cgtacaactt ctccacggtg acttggtgat tcctcgagta atgggtgatc gtttacagat
     2761 ccatccgatt acgatcttaa tcgttttact tgtcatgggt gatctaatgg gaattgtggg
     2821 cgttattttt ggtattccta tctatacgtt agtcaaatta ctcgttactt ttattttccg
     2881 taaattcaaa caacgctaca ataaatttta tggcgataaa ggagagtatg aaaactcttc
     2941 attttctgaa gaagattatt ttaaataaaa cacaaaacgt cgatcttacg agaatagctc
     3001 gtaggaacga cgttttgtgt ttttgtttta atttttcagc aaagcaattg cctggtatgg
     3061 ttttagctcc agactttgat gaagaacagt atcggaataa ttatcgataa gaatattggc
     3121 ttctgtgaat ccttctggga tggcgatcct tgtatcttta tcatagaaat ttgttaatac
     3181 aagcagctgc tgtccttcaa attggcgaag aaagccataa acttccggat gatttgcttc
     3241 ataagcatga taatctcctt gtgcaacgat tggatatgtt tttctcagcc gaatcaattt
     3301 cttgtaatag gacaaaatag aatcttcaga agatagttct ttttctacat taatctcttc
     3361 tagcttgcct ccaactttta accaaggttt ccctgtagta aatcctgctt gttctgtgtc
     3421 tgaccattgc atgggtgttc gagagttatc gcgagatttt gctttgatcc gacgaaacgc
     3481 ttctttttct gtgaatcctt gttcaaggag taacttataa gcattcaaac tttctacgtc
     3541 aatatagtcg gaaatcgatg aatagtctgg gtcgatcatg ccgatttctt ctcccatata
     3601 aatatagggc gtcccacggt tcaaatggat catggttgct agcatctctg ctccttgttt
     3661 tcgatactga tcgataacta caaatcgatt gagcgcacgt ggctgatcat gattattaaa
     3721 aaatagagca ttccagccgt taccctcact catattttct ccccatgtat gaaatagtcg
     3781 ctttaattct gcaaaatcaa atggaggagt agaccattta ttcccatttt cataatcgac
     3841 tttcaaatga tggaaattaa agaccattga taattcttct ctactaggtt ctgtatacaa
     3901 gatacaattt tcgatcgtcg tagagctcat ttcaccgact gtgatgatag tggaatccgt
     3961 cccaaacgtt gcttggttca attcttttag atacgtatgg gtgattggtt tgtccgtata
     4021 ttcaggtttg ccatcatttt ctttattatt tttcaattgt tcgtctttac cgataacatt
     4081 gataacatcg aaacggaatc cttttacccc tttttccatc caaaaacgaa caactttgaa
     4141 tagttcctct cttactgctt ggttccgcca gttcaagtca gcttgtgtcc gatcatataa
     4201 gtgcaaataa tatttccccg tatctccaaa tggcgaccaa gcacttccac caaattttga
     4261 tacccaatcc gttggctctt ctcttaaaat gaagaagtcc tgataatgct ggtcccctgc
     4321 tagtgctttt ttgaaccatt catgttctgt cgaaacgtga ttcagcacca tatcgagcat
     4381 gatttctatc cggtatttct ttgcttcggc tattagctgt tcgaattcct ccatcgtacc
     4441 aaagatagga tcgatggctg tgtaatcaga gatatcataa ccgttgtctt tttgagggct
     4501 tggataaata gggtttagcc aaatcatgtc gatgcctaga tcattcagat acgggagttt
     4561 ttctttgatt ccttttaaat ctccaattcc atcaccatta ctgtctttgt aagatttggg
     4621 atagatttgg taaatgactt tgtctttaaa tgtcatggat atttacctcc attcaactga
     4681 tttattacct accacagctt atatatacaa gttatcattt attattataa ctgaacagaa
     4741 agcgtttgta aaatttatct ttatacattc taagaggtct gttgcccaat aaaaaacaag
     4801 agatcagaca tgtatttgtc tatcctcttg tttaaaatag caaaatattc ttgtttcttc
     4861 tataaatggt gttcaaaagc ttttatctac ggcaatcagt cttctcaaat gtaaaacatg
     4921 aggagctaat tttatttgag tattcttttt gctcgatgca ggacggcttt tttacaacct
     4981 ctgattcatg gtgttcaatt agcagctgac cctcgttatt agctcaaaac gctgatttca
     5041 caacctctgt ttttattcat tcgatttcaa cgcatcaatc atatcgatat gctttaattt
     5101 gaaatgggta acaatcatca caataaccgt aaatacgata gttaatcctg ctgataagac
     5161 ataagcccac catgtgatca ctaatggaaa gatgacattc tccatagacg cttgttgcaa
     5221 gatgaagtca gtcaatatat atcctacacc aaatcctccg atgattccta aaagagtaaa
     5281 gatgatattt tctcgaacaa tgtacatcgt gacttcttta tcaaaaaatc caaggacttt
     5341 tattgtagat aattcgcgta cacgctcgga aatattgata ttagttagat tatacaagac
     5401 gataaaagcc agcagtcctg ataatacgac aaagattaag acaaccgagt ctaagttcgc
     5461 cattgattct tcttgcgttt cgatttgttt ggacatgaaa gacgtatttg tcacagcgtc
     5521 tgtggcaagc agtttctcgg aaagcatttc ttcttctttt gttgtcatct tatctgtacg
     5581 cagcaaaaat gcattatcca tcatcgattg atcagtaact gaagcataat attcagggga
     5641 catataaata aagctaccta gatagttttt cacgatttct tttacttcga ctttccattc
     5701 tttttgatct tcatcataaa gcgaaatatg atctcctgta gataagtcaa atagttctgc
     5761 tactttttgc gtaatagcga ttcctgtttt aggcaaggag cgtgggttct cttcaagatc
     5821 agttaaatgg ataaaacgat ccaaatcagc tgttttactt ggtacgatca ctgtcagact
     5881 ttgcgaagca tgtccttttt ttctcaattc tacggtttga gtatgaatag ctaggatctc
     5941 atttactttt tcttgcttct gcagcatatc cgctgctttt tgacgactat cttccgtgga
     6001 tgaatccaat gtaacgattg cttgatagtc aatgatcggt ccaaattgtt tggctgaaac
     6061 agaacttaga gaatctttca atcccacgcc tgcaaccatc agcccggcac atccagcaat
     6121 tccgataatc gccataagca ttcttgactt atatcggaag agatttcggt aactgatttt
     6181 ttgattaaag ctaaggcgtg accacagcgg agtgatgtat tcaagaaaaa tgcgtttccc
     6241 tggcttcggt gctttcggtt gaagcaatgc agcaggccgt tctcttaaat ctttgaataa
     6301 aacgatcatt gcagcgccaa atgcggcaat gaagaatgcg gcagctgctt gtaaaatcgg
     6361 cggccagtca taaaaaataa gcgcactccc aatatcatac cgctcgttag acagctcaaa
     6421 aatgatcctt ggcaaaagat tcgttcctaa tatcgttcct aaaacaatac ctatccctga
     6481 ggctagactt gcatagattg catatttttt tgcgatttca actttcgtat aacctaacgc
     6541 ctttaatgtc ccgatttccc tgcggttttc ttctaccata cgtgtcattg tcgtaaaggt
     6601 gatcaatgcg gcaataaaga aaaagaaaac aggaaaaaca ttggcaatcg cagcgatacg
     6661 atcagacaga tcaccaaatt cttgaaaacc gacattttct gaacgttcat tgtataggta
     6721 tgatggttta gttaaatttt caattttttc tgcatttcga ttcaattctt cctctgcaga
     6781 ggccagttca cgttctttct caactaattg cctttcttgt tcagcaagtt gagcttcttg
     6841 ttgaggtaat gtttgaacag cgattgttcg ttgcgcttca ggaagctgct ggatagttgc
     6901 tgactgttgc gctaactgtt ttttagcatc ttctaaccgt gttcttgctt gcgttgtttg
     6961 tgctttgcca tccgctattt tttgctgtgc cggttctaat tcttcgttgg ccgatttttt
     7021 cagctcttct aagcgttctt cgggtcgatc ttgtaaatac ttttctgcct tttcttgatt
     7081 ttgctccatt ttatctttgt aagctttgct gtacgtattc aaattcttta cgtttgtaaa
     7141 atcaagatat aagacagaat aaatatctgt tttaaaaacg gattcaggta aatagacaaa
     7201 ataatcgacg ctaccatttc cgacgttcgc gtatcctctc tccaaattgt tgatgaacaa
     7261 tggagaacgg acaaagcccg tgatggtata ctctttttct ttcaattcat ctgaatcaat
     7321 ctgataaata tcaccgattt tatacccttc atcttccgct ttttcatcca atacaagctg
     7381 gtttggtttt tccgggagtt ttccatcagt taattgtagc ttgttctgct tgttattttt
     7441 gttataagaa aatatttgaa tcacttcgtt tttgccagta ttggaataat agatctgttt
     7501 tcctgtttct acttttgcat ctgggatctt ttctgcttga gcaatatctt tttgtgtcaa
     7561 gccaccagta gaaacgattt gcaaatcaga aagtcctgct gtaccaacat agtgatccat
     7621 cgttttttgc atgacaggtc cagcagtttt gacacctaca aataaaagtg ttcctagcat
     7681 gacaataagg atgattgcaa tgaatcgtcc tttagattgc cgaatttcgc gaaaacctgc
     7741 tttaagatat gttcttgttt tcataaaatc cccctaccat acaatctcat caatggatga
     7801 aggatgatca tttaattcaa cagaccttac tttagcatca ttaatgtgaa tgactctatc
     7861 agcgatgggc gctaaagcag aattatgtgt aatgatcaaa acggtattgc cgtgttgtct
     7921 gctagcgttt tgcaataatt tcaatacttg cttgcccgtt tcaaaatcca gcgcgcctgt
     7981 cggttcatca cataaaagaa gtttgggatt tttagctagt gcacgagcaa ttgaaaccct
     8041 ctgctgttcg cctcctgata attgggacgg aaagttattc aatcgatgag ctaggcccac
     8101 ttgcctcaat acctctacag gatcaagcgc atcaggagaa acttccgttg ccaattccac
     8161 attttctttt gctgttagat taggaacgag attgtagaat tggaaaacga accctacatc
     8221 tgttcgtcgg taagcagtaa gctgtttatc tgaaaattgc gcaatatctg tgtcatcaat
     8281 aatgatctga ccttcatcag gtgtatccat cccacctaaa atattaagaa tcgttgactt
     8341 tcctgctcca cttggaccaa gtataatcac taattcgcct ttttcaattg aaaaagaaat
     8401 atcgtcattt gctgtaatgg tcgtttctcc cattttgtat cgtttgtatt cattttttac
     8461 ttcgacatac ttcactctga ttcctccatt ttcacgatat ttctcgctat atctatgcat
     8521 cgaatgcgtt ttctcacact tatttatttt accactgaat ttctctagac taaagaaagg
     8581 aatcctttaa tttagttgac aaataaacga tttatgattt acttacataa aaaacattgt
     8641 tatttgaaag aaggaacatc atgaatgatt taaaggaaaa tatgattttt gatttatcaa
     8701 aaactgcttt tgtcgcgatc gacatgcaaa aagagatcgt aaataacggt acactctctc
     8761 cacacactgc agcatctatt ctgacaaaca atgatttgtt agtaagaaca ttgaaacgta
     8821 ccgctgcctt gaaagtttta gtgaacgtag acatctccac tttcccttat ttgagtcaac
     8881 aaactgatat gggtggaatg actgcttctg tacctccaga attcactgat ttacttttga
     8941 aggattcttt gaaagataca gataatatgt tgactatcaa aaaatacaat ccaagtgctt
     9001 tttttggtac ttctcttgat cttcaacttc gccggcgtgg aatcgaaact atcattttaa
     9061 gcggtgtagc gacaacaaac ggtgtatatg ctactgcatt agatgccttt caacacgggt
     9121 accatatcgt gcttgctgaa gatgcttgca gtgatcgtga caaagaatcc catcaattgt
     9181 tcataaaaaa gatatttccg aaaactgcac gagtacgctc aacgaaacaa ataatcgaag
     9241 ctatccaaca aagcaaataa gtactggcac acgtgatttt catttacttc tttttgattt
     9301 catgggaaaa taaccacagg atttagatag gagtatgaac atggtgaaaa aaaagatcac
     9361 aatcaaagaa gtcgctaaac tttccggcgt ttcaatcaca actgtctcct tgatattaaa
     9421 tggaaatacg gagaaattca gtccgaaaac tgttcagaaa gttttagctg ccaaagaaga
     9481 attaggctat cagcctgact attttgctag acaaatgatt accaaggaaa cgaaaacaat
     9541 tggtgtactc gttcctgata tcacgaatcc attcttcagt acactgatgc gaggaattga
     9601 ggatatcttg tataagcaga atttcgtcac gatattatgt aatgctgatt ctgatcatca
     9661 aaaagaaatc gaatatctag ccgagctcac acgtcgaggt gtggatggat tcattatcgc
     9721 gacttctgct gtctctacag atgcaatcaa cgaaaatttg aaaaaacaag gccggccctt
     9781 cattgttttg gatcagaaaa aatctgaagg atttagtgat gccgtacgaa cagacgattt
     9841 tcgcggtggt tacctagcgg gcatgcactt gcttagtcta ggacatcaaa cgatcgccct
     9901 tgtctatcct gaaaatccac cggaaaatgt ccacgctcgg attgaggggt ttaaatctgc
     9961 tcttgatgtt taccaaatcc ctcacgacca actgatcctc ttacctacac agttctcaaa
    10021 acaaggtggc taccagatca cagcagaact tcttgattct gctgcaactg gtgtgtttgc
    10081 tttaaatgat gaattagctt ttggattata tcgcggatta gaggaagctg gaaaaagcat
    10141 ccctgaagac tacagcatta ttggctatga taatatagat atgtgcgagt atatcaagcc
    10201 gaaactgaca acgatcgcac aaccaatctt cgaattggga caaacatctg ccaagttatt
    10261 gttagatcgg atccaattcc cagaaaaaga atgggaagaa aaaagattac ctgtacgatt
    10321 agaaaaaaga ttttcaactg cacccttgaa atagctttca aggtgtgcta tatttttatc
    10381 gaactattaa aacgttttac taaaacgttt taaaaaataa ttttcccatc aaaataaatg
    10441 agatagttgg aggtttgtaa aatgaacacg gttacagtaa ttggaagtat taatatggac
    10501 actacccttc gtttaaccaa catgcccaag ccaggcgaaa cgatgcatgc gcatgagatt
    10561 tttcacgctg gcggaggtaa aggtgccaac caagcagtgg cagccaaacg ttcaggcgca
    10621 cggacatctt ttattggagg cgttggggct gacagcgaag gacaacagtt attagatcta
    10681 ctgacaaaag aaaacatcga tacatctggg atagctgaaa tccaaggcgc cacaaccgga
    10741 caagcaatga ttatggtaga cgcagcagga gaaaatagta tcttgatcca tgcaggggca
    10801 aataatgctt tccatgaaca agaagtccta aaaaacaagc aactgattac aaacagtgac
    10861 tttattattg ctcagtttga aagtagctta gatgcgacga tcctagcttt ttctattgca
    10921 aaagacgcag gaaaaacaac gatcctgaat cctgcaccag ctcgtgaaac gattcctact
    10981 gaactacttg aaaagacaga tatcattata ccaaatgaaa cagaaacaga aatcattaca
    11041 ggaatcagag tgacagatca taacagtttg gtcgcagcag ccgaaaaact ccatgaatta
    11101 ggtattggaa cagtaatcat tacactaggc agtgccggcg ctttctatca tacagaaaaa
    11161 gaacacggta tcgtccctgc ttttaaagtg gatgccgttg acacaacagc cgcaggtgat
    11221 acatttattg gcgcattaag cagcacactt cagccagacc ttagtaactt gaaagaagcg
    11281 atcctttatg gaaatctagc ctcttctgtt gctgtgcagt cttatggtgc gcaaccttct
    11341 atcccttacc gcgaagcttt agaacaatca gcagtctaaa attaacaatg agaggaagaa
    11401 acaaacatga aaaaaggaaa agtaatcaat tcagatattt cacgagtaat cgcacaaatg
    11461 gggcactttg ataaattaag tatcggggat gcaggcatgc ctgtcccctt tggtactgaa
    11521 aaaatagatt tggctgtctc aaacggtctt ccaagttttt tatctgtttt agaaaacgtt
    11581 ttagaagaac ttgaggtaca atcgattgaa ttggcggaag aaataaagac aatgaacccg
    11641 gaaatattgg aaaaaatcaa attattgtta ccagatacac cgattagctt tatcccacat
    11701 gaagaaatga aaaaagagtt gaataactgt catgcattta ttcgtacagg cgaaatgaca
    11761 ccttacgcca atattatatt agtgagtgga gtggtcttct aatggaaacg acaatgaatc
    11821 ctgaacagat ggtactgtcc gagcagattc atatgctgat tgaacagttg ggaggaaaag
    11881 acaatattga ttctgtcctt ccatgtgcga ctcggcttcg tatcaaattg aaaaaacagc
    11941 cagtagccaa tgcagaaaaa ttaaaacagc aaatcccttc cattaagtat ttcattttac
    12001 tcgatagata tgcagaaata atcatggatc agggattggc tcctgcttac aaggaattga
    12061 cgcgtcaaat cgatcattca gctccgaaag aaaagctaaa tccgatcgaa aaactatttg
    12121 gtttgatatc ggaaatcttt acgccgatca ttccgctgtt tgccggttct ggtatattaa
    12181 aaggtctggt tgttttagcg actacgattg gctggcttag tgaaacaagc ggaacttatc
    12241 atattttgtc tgctgcgtcg aatgccgtct tttattttct gccaatcttt cttgcttttt
    12301 ctgctgccaa aacgtttaaa gtgaatggat atattgcggc tgtcattgct gcagcactga
    12361 ttgaaccgaa tctgacctct ttactttcag acagtggcaa agcagctact gattttctgg
    12421 ggattcctgt agtactgatg cagtatacgt ctactgtcat gccggcgatt ttaggtattt
    12481 tcttttattc ttttgttgaa aaattcctca aaaaatatat tcgcgaaaat atgcaattag
    12541 tatttgtacc tttactttca ttagttatca cggttccttt gacgttaatg gtattcgggc
    12601 ctgcgggtgt ttaccttggc gaaggtttag ctgccgcaat tacttggatg atggatatta
    12661 acggaccgat cagtggcgca atcatcgctg gtggttggaa tattttggta atatttggta
    12721 tccaatgggc tgtaaaccct gtcatgatca gtaatatctc tgcttatggg ttcgaccgaa
    12781 tcgttccgtt gacaggagca gccaattttg gaatggctgg tgcagcttta ggtgtatttc
    12841 tgcgaagtaa acgttcaaaa acccgttcga tctccggttc tgcttttgct tcgattttac
    12901 ttgcgggagt gaccgaacca actgtttacg gaattgcgat cccattgaaa aaaccatttg
    12961 ttgctgcttg cattggtgca gctgctggtg gagctgtaat gggatttgcc caagtgaaag
    13021 cgatcgcttt tgtatttggt agtctgacca cattgcctgc attcatttcc ggtacttttt
    13081 tctggtactt agcaggactg gctgttagtc tagtcgtagc aatgatcact actctcgtta
    13141 gcgggtttga tgaagaccta atgtcttatg aatagttaat gaaaaagact gaagtatgag
    13201 atgtttatca catatttcag tctttttctt tttaggatca ttatttttat aaatttttgt
    13261 gatactttcc aaactttggt ataatttaca tatgcataac catttttatc cccacttgtt
    13321 tatccccaaa caaaggtggt ctatgcaata aaatgaacgt caaagaaaaa cgaatgaatg
    13381 ctcgttgctt atttacaaat tgttttattt agaatacaca gacagattta tgaaaagaaa
    13441 aagcttttcg acacgattat ctgttccaaa aaatgtctaa tgaatgctga ataagacaga
    13501 aaatcaatga aaggaaaagg actagtttat tatgaaaaat aaaaaaacga tacgtttgct
    13561 agctgctact tggctgattg ctccgtctgt tttgtcgacc aataccgtat ttgccgtaga
    13621 taatcaaata gctgatcaaa cagtcacaac agatacgact acatccagct cagctactag
    13681 tgaaacaacg gattctttta cagaagaccc aactggaaca actaactcaa caacaggatc
    13741 gaccgagcca actgaaattg atccggctga tccatcagaa aatgaaaagc cagccgagac
    13801 atattccctt acaattgctc cttctttgat tggtaagatc caactgactg cttcgacagc
    13861 ggattcccct gaacagcaac ctttggatat tacggaaagt ggaacggtag aaggtttatt
    13921 agcggaaacg aaggtaactt acactattac gccagctgaa ggtgaacaaa ttgcttcagt
    13981 cgctgttaat ggtgccaaag aagtaaatta tgatgcaaag acctttacag taggtacgaa
    14041 caatttatct attactgcag aatttaaagc tggaccggat acttcaacag agaaaccaac
    14101 tgatcctagt gaagaagtcc ctagtacacc agatccggaa acgccaaaca aaccagatgt
    14161 gggtaataac aacaataata atggttcaaa taattcaaac aataataaca ataattcgaa
    14221 cagcgggaat tcttcgaatc aaacaaaccc atctggatcg aataacaatg gaaatactca
    14281 gagagaacca aaccatccaa acaatggttc atcgaatggc ggaacgaaca atgcttcatc
    14341 tcgtccaaat acagcaaatc aaagcggtat agagaatcca tcttctgctt cttctgattt
    14401 tgttgtaaaa tcaccaattg atgctgtatt gcctacaaat acaacgaatg tccaacaagc
    14461 aatcgttcga gaagcattca agcatcttgg aaaaccatat gtatggggag caaaaggtcc
    14521 aaatactttt gactgttctg gtttgactta ttacgtatat atgaaagcaa ccggtcatta
    14581 tatcggtggc tggacaggcg aacagcaata tgcaggtacg cagattccag taagtcaggc
    14641 ccaacctggc gatctcgtct tctggggacc ttcttccggt gttactcacc atgtaggtat
    14701 ctatattggg aacggacaat ttatccatgc gcctcaacca ggagataaag ttcgagtaac
    14761 ttctatttca gatttcacgc ctgattttgc tgtccgagta aacttagcag gtttacctgc
    14821 tgcttcaggt tcgctgacag gcggctcatc tattttagat ggattagatg gctcgttcca
    14881 tttcagtcaa aaccaaacaa ctgatcagtt cttgaaaaag attgcagatg atgcacagga
    14941 aatcgggcaa aaagaaggaa tctacgcatc agtcatgatg gctcaagcta tccttgaaag
    15001 tggatctgga aacagcttat tatcaagcga accaaatcat aacctttttg gtatcaaagg
    15061 atcatataaa ggaagcagcg tgaccttcaa tacacttgaa caagatagtt ccggacaaag
    15121 ctaccaaatc cgcgcacaat tccgtaaata tccttcttac aaggaaagtt tggaagacta
    15181 tgccgacttg atcaaaaatg gactgactgg caatcctgat ttctacaaac caacttggaa
    15241 gtcggaaaca aaagactaca aagaagcaac caaatatttg gaaggacgtt atgctacaga
    15301 tcgccaatat tcacaaaaat tgaacgcgat catcgaagcg tacgacctga caaaatatga
    15361 tgaaccgaaa aaagaagcag caacaactga agaagcaaac gctgaaatcc tcaatatgtc
    15421 taagcgattc gatgtgccaa tcaaatggac aacagatggc gtgccaatca caggattaga
    15481 attttcaacg ataaaacgcg tcaacaacgc ttcttccatc ttccgtgtcg cttcaatctg
    15541 ggatttgtgg aacaatttta cggcaagaca gataccagaa acaactactc gtacagttaa
    15601 agctgcaact gttcctgtac taaccttgtt aagtttagac aaatcattct ggcttgatct
    15661 gcctttaaag aaatagaata aattaaaaag gggatgtgac actgtcacac tcccttttta
    15721 atttattcta tttctttata tttattttgt attccgttac aaactcgcgt acttggttag
    15781 gttccagagt gacatctcca aaactttcaa gtcgctccgc tcctggcagt tcttgagtct
    15841 caaatgtgat accgccatga tttactaatt tattcccgcg catttccggt ccatcttctc
    15901 caaaatttgc tgtaaaaatc acgattcctg aagcatctgt ttttacttgg atcgatactt
    15961 tttcatctgg gcttgtcaat tccgcagcat ttagtaaacc agattgtttt ttcaacaaaa
    16021 atggatgatc gatgccgccc actatcattg attggcgaaa atcactggca aagacctcac
    16081 ctaatttttt aggacgttgg aagtcaaatg ctgtgccact tactgaaact acttctcctg
    16141 taggcgttgt atctttattg agcgtagcaa ataatcggct attcaaccaa agtatatgtt
    16201 cgtcgattga ctgtgctgga tcaccggtta aattgaaata gacatgattt gtaggattga
    16261 atactgtttt gcggtcactg atcccttgga ccgtcactcg ccatgtatca tcgttcgtca
    16321 gcgtataccg tacttctaca tctaaagttc caggaaatcc gtttcccata tctgggctag
    16381 tctgatagaa aatgaccgat gcttcgtttt caccattgat gactgtataa tgccactgct
    16441 tggcttcaaa gctaggtgca ccaccatgta aacaatgacc agtgtccggg tctgttttta
    16501 cttcgaacgt ttcatcatct aatttgaatg tgccattcgt aatccgtcca gccatacgtc
    16561 cgattgaagc accgatataa gggtcttttt ctagatattc ttttgcttca tcaaaaccta
    16621 agatcaactc tcgtttttca ttatcgatta ttgtttgaaa atgaacaacc cgtgccccaa
    16681 gatcactgac agacaaagat gtcccatttt gattcgtgat cgtaatcaag tgatagtttt
    16741 cgccaaaatt ttgttcattg atttccatgt tccttgcacc ctcttttctt tcatttgcac
    16801 tgattccagt atacatggaa accgttttct taacagctta ttccgtgagt aacgagaaaa
    16861 aatttactat attctaataa aagaccgtga aaaaaaggtt gtgacaaaaa tattgtcaca
    16921 acctcttttt ccgaacaaac ggtgttcaaa agcttgcatc tgcggtaata agcccaacca
    16981 aacgaaaaat atgaggagct atttttgctt ggctgttctt attacttgat gcaggacagc
    17041 tttttcacaa tctctttttc aatacgattc cagtatataa ccatgtcgtc agtacatagt
    17101 agatgaaata caatacgaag aagatcgtaa acggaaccca aatatctttg tatggatttt
    17161 gcatgaatag accaaacatt tgtaatccga ataagacatg gactacacct aagattcctg
    17221 gtgctaagaa taacacgcct acttctctac ggatcgattg tttaagcaac ccgcgtcgtg
    17281 ttcctatctt ttcaagcatt tcataacgag caatgtcact cttgcttcct gacaagatct
    17341 tgaacatcaa acagctagca agcatggtca agaaagctaa acctaggaag aatcccataa
    17401 attcaaaacc agagaataga ccattataca cttcatacat ttcatatttc tggttgctgt
    17461 actctacttt ttgcaaagat ggattgttca ttttattttg agcaacaagt gtttcgattg
    17521 gtttcaggtt tgcttggaag tcttttacct gaactagctg taaagtggat tgtggcaagt
    17581 tcagttgatc aaattctgat tgactgacta ccttgatttc tttttcttgc tgttccggca
    17641 attcatagct tcgaagttcc tcttgtacag tcagttcatt cattttttta gcaggaactt
    17701 tttctttcgt gatcttcggc ggtgttccat cttcatgttt tatcatgatc aatggttgag
    17761 ccgcaaattc acttgcatta tagtaaatcg ttcccgcatc ttctttttga gtataagaag
    17821 cagaaagtgt tggtgaaagt tctttgattt tttgctgatc ttctgcccga ggattattca
    17881 tcactagatc atatccagaa ttgttttctg tcaagtctgc aatttcattt ttaaaaccta
    17941 atcctaccgt caaagcgccc agtgccaagg caaacagcat agcgaccatt gagagcattt
    18001 gtgtatattc acgaatacga aagtttaact gtgacaatgt gaaattgttc aattttttca
    18061 atgaaatctg ttctgatttc tttagtaatg ataaaaagaa aatgatcaca gaatggaaaa
    18121 tgaaataagt tcctaatatg atcgtaacta aggctacccc tataccaaag acttgatagg
    18181 tccccacgtg tttcatcata tagtaaccgc agcctagaaa agcaattccc agtactactt
    18241 caaggaaaaa caagaattta tgctgcttga ttcttgttgg tgtctgatcc gcacgaatca
    18301 atgctaaaat cggttttttg acaatcgaaa aagcattgac aacagcagct aataaaaaca
    18361 aaacagcaaa aaacaaaaca gtgatcaaca acccgtgaat actaaaaggt gtaaaatgcg
    18421 aaatattgag atctaactga tccactaata atcggtggac gatacttgtc aatcctacac
    18481 cgacagctga acccactaaa gtagctgcga tccctactaa gaatgtttca gaaaaaatca
    18541 tctgtgcgat ttttctgcct tttgctccca gcatcataaa cattgcatag tctttttgcc
    18601 gcatcgtcat caaaaaggaa tttgcatata aaatataaac gaatgtgatg atccctagta
    18661 aaacagaacc gaatcggaaa atgataactg tagccgcaac gatcgtatta cttttcaaaa
    18721 aagcttcgtt gcttgccatt gattcaaaca tgtaaaaaat agcagatgcc ataactaatc
    18781 ctgagaataa caccaagtag tctcttaaac ggctcttgat cccagtaagt gataatttcc
    18841 aaagcatagt caatctcccc ctactgttcc aagtttccaa gaatagatag tatttctcga
    18901 tagaaggaat cacggctttg atctccacgt ttcacttctt gatggatacc gccatccttg
    18961 atgaataaaa tacgttggca ataacttgca gaaaacggat catgcgtcac aagaagaatc
    19021 gatacatgat cttttgtatt caattcgtcc atcgtatcta aaagatcccg ggcacttttg
    19081 gaatccagcg cacctgtagg ctcatcccct aatagaatcg ttggttgtgt gatcaatgca
    19141 cgtgcggcgg cgacccgttg tttttgtcca ccggaaatct cagaaggata gctttccaaa
    19201 atatgagaga tggataaacg ttctgcaatt ttttggacac gttgcttgat ttcttttggt
    19261 ctgacgcctt gtaaagaaag aggaacagcg atattttctt gtgctgtcaa gctttctaat
    19321 aaattgaaat cttggaaaat aaagccaatc tcattcgcac gaaaatctgc tagttgattt
    19381 ccttttaatg tggtcacatc tttttgattg attcggatat gcccatctgt tggtttatct
    19441 aatgtggata aaatattcaa aagtgtcgac ttaccagaac cagaagcgcc catgatccca
    19501 ataaattcgc ctttttctac atcgaagctg atgccgctta atgcttgcgt ctttttctca
    19561 ttgttttttc cgtatacttt tgttacattc tgtacttcga ctattttgtt catttctttc
    19621 cctacccttt aattagtttt tccagtgctg ttttcggcac gtcttttttt tcgacttctt
    19681 catacgaagt gacaccttta tttttgttcc aggtgatttt taaatatgct cctttacgaa
    19741 gcggtcgagt caattgtccc ttgaagtcca gttctttttc cttaccctct ttcgtatagc
    19801 caggcaggat atatctatag ttgactgttt cttgtccaga gtctgatttt gaaactgttt
    19861 ttgtaccatc tgtcgtaatt tgtacatagt attcttctcc acccataact acgtaatctg
    19921 cgattttcac ccctgcaagt accacaacga tgaaaacaag tagaccgatc agaagttttt
    19981 tcactttttc cccttctctc ttactttata tactgattat agaaaaaagc gaacaattct
    20041 gccttcgaat caactatcat tcttctttca attttgtcat tttctttaaa acaactaaaa
    20101 aaagagttca agacaataat ccgtcctaaa ctccttcttc ctatcttaca aaaagtatgt
    20161 cacttacggt ctttcatgtc gcctgtattg ataacaggtt gcgttccttt gtctgtaatg
    20221 attgagacaa gtaaattatt gatcaattgg actttccgat catcggtgaa attgatttct
    20281 tgtccatctt cgatttgctc taaggccatt tgagtgattg tgacagctcc ttcaacaatg
    20341 gtttgtcgag cagacaaaat ggcttttgct tgttgacgtt ggagcatggc actagcgatt
    20401 tctgttgcat aggctaaatg gttcaagcgt gtttcgatta cttcgacacc tgctactgct
    20461 aaacgctctt gcagctcttt ttgcaactcg tcagacactg ccgtcgtgtt tcctcgaagc
    20521 gtcaaatcat tctcattgaa tgtatcatac gggtattggc tagcgatatg ccgaatggct
    20581 gtctcacttt ggatttctac aaaatcttga taatacgcta catcgaataa tgcctttgct
    20641 gtatcaacga ctttaaagac tactactgct gagatttcga tggggtttcc atccaaatca
    20701 ttgactttta aaacagaact attgaaattc cgaactttca acgaaacagt cattttttgt
    20761 gccaaaggta tcgtaaggaa aaagccattt tctcgaatcg tacctaaata ttgtccaaaa
    20821 aacaaaatta cttttgcttg attaggactg actacagttg ccgaacttaa caacaataac
    20881 gtgatcaacc aaagaaaaat acttaaaaaa agtgcccaaa ctttcgcttg ccacatacct
    20941 aaatagaaga aaaacaatcc aactaaagct aatacgacca atccgacaat ccctgcataa
    21001 ccattgatat aaaatgctct tttttctctc atgctacttc ctgcgctcct ttatccttca
    21061 ataaatatct aatgttactt ttttaatttt ttttcgaata cgcgccatta caccttctag
    21121 ttctaactct ttgtcttcag tgatgtacca accccttgcc tgtgtccctc cggctgcctg
    21181 cggtaaataa tccaattttg ttcgaaccca gtgcttttct tctgtaaaat agtcttctac
    21241 caagacctct agatccatcc ctgctgaaag aggcaaatct tcccgaacag atttaaagat
    21301 aaatcgttcc ttcttcccat catacttcaa tattcctgta tcgataatcg gtaaagaata
    21361 ataatccagc tctttcttca ccgtcaataa atgtttgaaa acttcatggt acacttgctc
    21421 taattggcga ccttctgaat tgttcgggta attaaccatc actttttctt tgtctggatc
    21481 atagaaggtt tggcttagtt gagtgatata agtatgtaat ctttccattt ctgtttttaa
    21541 cttttctctt tgaccttctt ctaccttcat atactctttc accctgtttt ctttttagat
    21601 attttctttc ttattttact atagttatgt gcgttaagcg atgaaaaaca gcaaagattg
    21661 gaaggatctt tgctgttttt tcttaaaata tccagttgag tacaggatca atctgtttca
    21721 ttgtcctctt gatccgtcgt aatattgatc gatgtgtctt tagcagtgat ctttagcttt
    21781 ttctgtcctt gttctaacaa agtatttgtt attggctggt agtctttttc aatataagat
    21841 tcaccagacg tttggatagt atagccactg ttttccatct catgcaaaga taaattgcca
    21901 ccgtttagga tgacttttcc ttctttgctg atcaaactgt cttctagatg gaagttgctg
    21961 ttttcacctg tgacgatcaa gttatccaca ttggcctcat ctaatcgaag tgagcctaat
    22021 tcatctttcg cagttattgt tcctcctgta aattcattga acgataccca gccatcatgt
    22081 gtcttagtat tcactttttt gattgatact tcgttgatat caattgcact attattccct
    22141 tcgattgtca attcagataa ttcatgatct gcagggacaa caatagtgat ttgtgcactt
    22201 gtgtcttcta aactaatgat tgcacctgtt gattttttga ctgcattcac agtcaacacg
    22261 ccatttttct cctttatttc tggcttttct gtcttatcat aagtcgtttt tatctcaaaa
    22321 tccaatccgc gtatgaccga aactgtttga ttttctgcat ggatgacgat tttgtttgca
    22381 tccttgattt tttttgtctc agtaaccata tcgatcaacc gaggaccatt atcccaagtc
    22441 agactctttt gagcgcctaa tgtataagtt gcacctgcta ggaggccacc agtaagcatc
    22501 agaccaatac cggtaatcaa aaaatgtttc aatctcaatt tatgtgtcct ccttcaaacg
    22561 cccgctgaat gcagatctct ttttgtaata aaacgtcgtc ccatccattt aacaaagttc
    22621 atcatgacat aaaaaagccc ttttgttcct ttcagcaaca gtggcgaaat aaatactgcg
    22681 cttcctgtgg cgaacagacc gacaccgata aagaataatc cactcagaat cgattgtcca
    22741 aacacactga ttcctgagat gatcatgaat aggccaacac ctaagatact gatacataaa
    22801 acatagatac taaaaatgag cgctagtaaa gcaaccaata gtcccccaat aaccaatata
    22861 aaccccagtg ctagtggaat gaggacagga gaagcaaata aaccgataat gattaaccaa
    22921 atcaggcgaa tttgtctttt tggatttttc actaactcgt tttctgcttg ttcttcttct
    22981 gaaaagtaac tgatttttaa ttttaaggca aactgctttg gtgtaccata ttcatcaatg
    23041 atctgatcta atgtttttcc agaatctaaa aaatattcct cataatactg gatcaaatca
    23101 aaacgatcgt cttcaggaat ttcttgaaga tgatccacaa cttcattcaa atattcattc
    23161 atttgtgctc tcctccaata accggtctaa agaatctcga aaaacaatcc attcatcaat
    23221 aatttcttct aaatgacgac ttcccattgg tgtcaacgag taatatcgac ggtttctgcc
    23281 ttcaaaaggt tcatcatagg tctcaagata tccttctttt ttcaatcttc tcaaaaccgg
    23341 atatagtgta gattcactga cagagagact ttcttgtacg atttttgtta aggcataccc
    23401 atacatatct tccttgctta atgctgctag cacagtacca tcaagaagaa cagttggaat
    23461 ctggatgtgc attacgtcac cttctattct caagtgtaat attttatttc aatctatatt
    23521 atacaacata taatatagtt tcaaaatcag tttgtcaaat acaaaaaaac tgactaaaag
    23581 atttgatcct ttttagtcag caaaaaatgg cttctctttg atttttatta taacagtggc
    23641 ataatccaat ttgtccacat ccagctcagt ggcatgataa cgatcattgc aggtatcatg
    23701 tcagctactg ggaacatttt cacgttgatc attctaaaac cggtagcgac catcaagaat
    23761 ccgccgcaag ctttaaaatc taagatcatg tctggtgtag tcaatggata aataaatttt
    23821 gccaataaga acaaaatata aaaaataatg aattggggaa ttgctatcac agaaacgaca
    23881 taccctaaat tacacgcaaa aatagctgct gtgaaaaaat caaggacaga tttagaaatc
    23941 aaaatcgtac tgtctcctgt catcccagaa tctaaacttc catagatccc cgtaccactt
    24001 gcacaaaata gaacaatgac cgtcactaat gtgctgataa attcatcgtg ggtcatacca
    24061 agcttttcac tagggaagat tttagaaata ggtacttgca tcagcgcagc ccccttattt
    24121 atcccattac ctaaatgaac aatcagacca atcgctgttc ctatcactaa agcaaaaata
    24181 acagccggca tatttttcat cggcgcaatg gaatagattc ccatccccat cgaacacaca
    24241 ccaaaaacca tcgtcagctg tgctttaaaa tgttcactta atttatttcc tactaatccg
    24301 ccaattagtc cgccaagcaa aacggaacct acattgatca aaactccagt tggcatcaaa
    24361 aaactcctct ctttttctac gaagcgaaaa aaatcagaaa aatttctgta attattgctt
    24421 tcgctgcaag aaagattgta acagacagag aagattacac caattcattt tttgtgttaa
    24481 agtattcttt attggaataa gtaggaggaa tatgatggat atccataaat tcgaagtttt
    24541 tcttgattta gccgaaacaa tgaatttcac caaaactgct gatcgacaat tcaccaccca
    24601 aggaaatatt tctaaacaga ttctttcgtt agaaacagaa ttagacgtca aattattcga
    24661 acgcgcccat agaaaaatcg aattgaccga agctggaagc ttattgctcc catatgtaaa
    24721 aaatgtcgta gaacaatacc atgctatgca agaagtgctt gaaacctata cgaaagacaa
    24781 caatctttca ttaaatatct taacgatacc gacaatgatc aattataaag gtttttccaa
    24841 aatcaccgag tttttgaaac gccatcctga atttacagtc cagttgaaag aagtggaaag
    24901 tgtcgaatta acttacaatg acgccaatga acatagtgac acgatccact ttgctagaaa
    24961 tttccaggcc ccaccaagtt cgattgagtg gttgcctact gaaacagacg attttgtagc
    25021 agttctacct aaaaatcatc ctttagctag taaaaagaaa ttggatctaa gcgaactgaa
    25081 aaaggagaac tttttgctcc ttggccccac tacgaacttg tatcaacctg tactagatct
    25141 gtgtcgcgaa gctggatttg aaccaaaaat ctcttataaa ggagcaagaa tcgacttgat
    25201 catcaatatg attgcaaatg acatggggat tgctattgtt atggaaaaaa cagtcaagaa
    25261 tctattaaaa gaaaatactg tgattctccc tatttctcca acaacagaga gttatcttgc
    25321 atttactcgt aaagtcggtg agcattcttt ggcttcagat acattttggg agtatttaaa
    25381 tcaagaagaa aactaagccc tacattttct gtccatctat ccacccaaaa aaagagagcg
    25441 acgcatgact ttcgctctct ttcttgcatc atgtccttgt ttcaatgaca cctctaacat
    25501 ttctcgtagt tcaataagaa agcagcgatt tttatgccaa aagaaccgta agaacatttc
    25561 cctaatcaca aaaaaacgac taaaaacccc taaaaacctt caatagaatt tttaggggtt
    25621 ttattctttt tcctattgtt tacttaaccg tatcattgct gtcgttccta ctccttcgat
    25681 actgctataa tcaatacgtc catcatgcag ttctagaatt tttttaacca gtgaaagtcc
    25741 tagcccattc ccagaaattt gacgacttgt atctccttga taaaattttt caaacattcg
    25801 atctctggtc tcttcattca ttcctacacc tgaatcggtc acttccagtc caatctctgt
    25861 ggctgtctca aatagtttga tatgaatctg tccattgatt tgattgtact ttatggcgtt
    25921 atccatgata ttcagccaca cttggtacaa aaactcttca ttgcctgtgt aatttactgc
    25981 agcaagttcg atatccaatt ctagctgttc tttctcccat ttaggctgca aaaaaacgat
    26041 tacttctctg atttgttcat ctaatcgata ctctttcttt tccagttgga tacgctggtt
    26101 ttccagtttg ttcaatttta agatgttctc tgttaattgt gaaagctgcg tgatactttc
    26161 gataatccga tataaaaata tttggcgttc ttcgtcagag agatttgggg cttgtaacaa
    26221 ttgaacatat ccttggattg ttgctagtgg tgttttgaat tcatgggata cactggacac
    26281 aaagtcattc ctcagtgtct cgatactatt gagttcttgg accataacgt tgaaatcttt
    26341 atacagttgc tgtacttctg ctactttctg ctgttcatcc attcgaatcg aaaaatctcc
    26401 cgttgctacc agactcatat ttgtccctag cgcactgatt ggatgcagga tacgccgccc
    26461 aacaaaaatc gatattcctg tacccacgac catactgaac cccgcaaaga cgaataacgg
    26521 gtataacgtt ggttttccag ccgattcagc aggcgagata gaatgtcgag acaataagaa
    26581 aataaaaccg agaaaacaca gcagtgtcac taagatcgaa atgaagacaa tactagcaaa
    26641 atagatccat aattgtgaaa atagcttttt tttcatcttt aaataaccgc cttatagcct
    26701 agtccccgta ctgtgatgat ttgaaaatct tccgtctttt caaaacgggt ccttaaacgt
    26761 ttgatatgta catcgatcgt tcgttcttct gtattcgtat ctaatcccca aatatcatcc
    26821 atcaactgtt ggcgagtgaa gatcttgttg ggataagaca gtaatttata cagaagcaga
    26881 aattcttttt gaggcagaat caacgaatgc tgatcttgta acactgtata agcatctagc
    26941 tctaattttg tttgtccgat tatcaagcta ctgctgtaat tgatctttga acgtcgaagc
    27001 aatgcttcca cacgtaggat catctcattg acatcgatgg gttttgtcat ataatcatct
    27061 gctcctaatt tgaacccgcg tttcttatcc tcaaaatcgg cctttgccgt aatcattaga
    27121 acaggtgtgt caaatctggt gctacgtaat gagtctagca agtcatagcc atccattttc
    27181 ggcatcatga tatctgtaat gattaattct atacgtgtcg cttctagttt tttcagcgca
    27241 tcctctccat tcaatgcggt gatgactgaa aagcccgctc gttccaacac agaacggtaa
    27301 agcaactgta attgcggatc atcttctact actaaaatac gattcattga tctccctctg
    27361 ttttttgaaa aatttctaga ttttttcttg tagttcatct tcagttaaca tttatttcgt
    27421 atactacgat cattgttctg atatacttat tagtataccg aatatttcaa tacgggagaa
    27481 gggtgattat gcttcaatta aaagatataa aaaaatatta taaagtcggc gaaaccacca
    27541 ccaaagcatt ggatggtgtg tctgtcgctt tcagacaaaa agaattcgta gcgattttag
    27601 gtcctagtgg ttctggtaaa acgactatgc tgaatgtgat tggtggacta gataattatg
    27661 attccggtga tatggtaatc aacggaaagt ctactaaaga ctttaaggac agtgactggg
    27721 atgcctaccg aaacaactct atcggtttca tttttcaaag ttacaacttg attggtcatc
    27781 taggcattat tgaaaacgtt gaacttggga tgacattaag tggtgtttct aaagacgaaa
    27841 aacgaaaacg ggcagaagat gcattgcacc gagtcggctt gacagatcat atgcataaaa
    27901 agccaaatca gttatctggt ggacaaatgc agcgagtggc catcgctcga gcactagcca
    27961 atgacccaga tatccttttg tgcgatgaac caaccggagc gttagataca gaaacaagta
    28021 tccagatcat ggaattgatc caagagttat cgaaagaaaa attagtgatc atggtcacac
    28081 ataatcctga actggcccat caatacgcag atcggatcat tgaattttct gatggtaaga
    28141 tcttaacaga ttcccatcct cacatcgaac gaccaaaaga tgatcaattt aacttacgca
    28201 gaacaaagat gagtttttgg acagcgttaa agctttcctt caacaatatc cgaacaaaaa
    28261 aaggacgtac tttcttgacc tcctttgctt ccagtattgg gatcatcgga atcgcgatcg
    28321 ttctgtccct ttcttctgga ttccaaaaac aaatcgataa cacgcaagct gaaacgatgg
    28381 cgaaatttcc gattactatt tctaaggtaa cgactaacca gactcgtgac gatgccggat
    28441 tgggtgcaag caaagcggat tatccagaca gcaaaacgat tactgcaaaa gtcagcgaag
    28501 aagataaagc acagcacacg aataagattg atcaaacata cgtcgattac gtaacagaca
    28561 tcgatcctaa cttaagtaat aatatcggat ttacccgtac tacaggaatc aatcttttac
    28621 gtgatgtaaa tggcaaagta cagcctgtca gcttctcgaa tcaaaatcct gatacggaat
    28681 ctctttcttt atctagtacg atgtcagcaa tgacaggtgt gggagtgtca agtttcccta
    28741 cacagcttga cactagcaaa gaaaactttt taaaagacaa ttattcttta cttgccggaa
    28801 gttatccagc ctcagcaaca gatgtcgtgc tgatcgtaga cggcaataat aatactaata
    28861 tcaacgcatt gaagaatctt ggatttgatg tgaaagaaga cgaaaaactt gattttgatg
    28921 atatcgttgg tacaacattc aaattagtaa acaacaatac ttactataca aaacttccaa
    28981 ctgggaattt tatccctaat acagattatg atgcgatgta tcagaatgct tctgatgaat
    29041 tgaagatctc ggggatccta cgcgtgaaat cttcttctac aatgaatctc ttatctccag
    29101 gaatcgctta tagcgatcaa ttaacgactc aaatcgtcaa cgaaaacaaa gaatcagaaa
    29161 tcgtcaaagc tcaaagagac agtggtgtaa atgtcttaac tactgaaaaa gttgacgaaa
    29221 atgccaaaca aacattgctt agttatttag gtggagacag cttgccttca agtatcatga
    29281 tttatccgaa caattttgaa gacaaagaaa agatcttgga ttatctagat gattacaata
    29341 aaggcaaatc agatgaagat aaaatcattt atactgattt agctggaacg atgactgaac
    29401 tgactggcgg attgatggat gctatcactt atgtccttat tgcttttgca gggatctctt
    29461 tagtaacaag tatgatcatg atcagtatca ttacttatac atctgtgatt gaacggacaa
    29521 aagaaatcgg tgtactaaaa gctttaggtg cacgtaaaaa agacatcact cgcgtatttg
    29581 acgcagaaac gtgtatttta ggtatttctt ctgggatatt aggtgtgttt attgcatggc
    29641 tagctacgtt ccctatcaat agcatcttat acaacatgac ggatctaaaa aatgttgctc
    29701 aactaaatcc tgtccatgcg attattctag tgatcgtctc tactatattg acgatgcttg
    29761 gcggtcatct tcctgcacga atggcagcga aaaaagacgc cgctatcgct ttgcgtgcag
    29821 aataaggttg tgacaaccac atttagattt gagtcataag agcgctgaaa tagtagaaaa
    29881 cagcaagaaa cgaggaagta atatgttcct tgtttcgcgc tgtttttttt aattattttt
    29941 tataattctg gtatactttt ctttcctttg taccacaact cttctggcgt atacctgtct
    30001 tttccattta tgaatactgt tccatctgct tcaatcgtta cttcagtccc aatccacata
    30061 ttacgagaaa gtgcgagaag attttttctt tttcctcttc ctttttttcc aaattgcaag
    30121 gcatcggtta tctcttctgt ccaatgagat ttcacgtatt ttacttctcc gttttcaaat
    30181 gtaactttca aaatattcgc tatattttcc acttttactg ctttaggaaa tgactccttc
    30241 atttttttca ctttccttcc tccaacacta agtatgcgct ttcttttagt gataagtata
    30301 tcatagttta ttctattatc aaaaaaacgc atccattcca ttgtagaata ctttaccgaa
    30361 caagttgaat tgttttcctc tttttcacct tttactatag ataggtaaag acaactaagg
    30421 aggaatctct catgacagat caaccgtatg atttaagaaa agtcatcgaa gaactaaaaa
    30481 cacttccagg tcaatatcac gaaacagata ttgaaatcga tcctgaagca gaattatctg
    30541 gtgtttaccg ttatatcggt gctggcggta cagtaaaacg tccgactcaa gaaggaccag
    30601 ctatgacttt caacaatatc aaaggtttcc ccggcacacg tgtgaacatc ggaacaatgg
    30661 ctagtcgtaa acgtgtaggt gaaattttgc accatgatta tcgaacactt ggtcgtttct
    30721 tgaaagacgc tgtagaaaat ccaatcaaac ctgtgaaagt agacaaagca gacgctccag
    30781 cacaagaagt cgttcatcta gctaccgatg cagacttcga tattcgcaac attttgcctg
    30841 cacctacaaa tactcctgaa gatgctggtc catacatcac aatgggtgta gtcttaggat
    30901 cagatccaga aaaaacaatg agcgatgtaa cgatccatcg tatggttcta gaagataaag
    30961 atacgatcgg tatgtacatc atgcctggcg gacgcaacat tggtcacttc caaaaacaat
    31021 tcgaagcctt gaataaacca atgcctatca ctatcaatat tggtttagat ccagcaattg
    31081 ccattggcac gactttcgaa ccacctacca ctccacttgg ctacaatgaa ctatggtgtg
    31141 caggagcttt gcgtaacgaa cctattcaaa tcgttgatgg tatagcagtc gatgaagtgg
    31201 gtattgctcg tgctgaattc atcattgaag cagaaatcat gccaaatgaa acaatgcaag
    31261 aagatatcaa cacgaactca ggaaaagcaa tgccagaatt tcctggctat actggggatg
    31321 ctaatccagc tgtacaagta gtgaaagtca aagcagttac tcaccgaaaa gacaatccaa
    31381 tcatgcaaac aacgattggt cccagtgaag aacacgtatc tatggccggt attcctacag
    31441 aagcaagtat tcttgccttg gtcgataaag ctattccagg aaaagtagta aatgtctaca
    31501 atcctcctgc aggtggtggt aaattgatga ccatcatgca aatccgtaaa gaaaacgaag
    31561 cggatgaagg tattcagcgt caagcagcta ttcttgcttt atcagccttc aaagaattga
    31621 agacagtcat tttagtcgat gaagatgttg atattttcga catgaacgat gtagtttgga
    31681 ctttcaacac tcgtttccaa ggagataaag atatcatcgt gatcccagga atgaggaacc
    31741 atccattgga cccatcagaa ttaccagaat ataatcctgg ctctattcgt ttccgtggga
    31801 tgagtgcaaa aactatcttt gacggtacag ttcctttcga tatgaaagaa cattttgtac
    31861 gtgcaaaatt ccaagaagta gaagattggg aaaaatattt gaaataatga atgatctgca
    31921 actgttttga catcataaaa aggagtgcgg aaaatggaac aattggcaca acggaagaaa
    31981 aaaatcgtga ttggtgtaag tggcgcttca ggcaccattt atgccatcaa cctagtgaag
    32041 aaactaaaag aatatccgat gatagaaaca cacggaatca tcagtccatg ggcaaaacag
    32101 aacttaaagc tggaatccga cttatcacta actgaattcg aaagcctgtt tgattaccat
    32161 tattcaaaca aagatttagg tgctgctgtc gccagcggat catttttgac cgatggaatg
    32221 atcatcgtac cggcaagtat gaaaacagtc gcaggtatct ctgttggttt aggcgacaac
    32281 ctgattgcac gtgctgctga tgtaacaatg aaggagcaac gaaagttagt cattgttcct
    32341 cgtgaaacgc cattaaatgc cattcacttg gaaaacttga ctaaattagc acgactaggt
    32401 gtgcaaatca taccaccaat tcctgctttt tataatcagc ctcaaacgat ccaagatctt
    32461 gttaatcacc atacaatgaa acttttggat caatttggaa ttgtaaatga gcattcaaaa
    32521 cgttggcagg gtgattaata tggaaaaaga aacgatagtt gaagtaacca aactaggttt
    32581 cacgatccaa gctgctgtta aagtaatcgg taaagactta ttgatcactt taacaggcgg
    32641 agatacacca catatcggaa cagtcactac attttctcgt gatacagaga tgcaaactgt
    32701 acgtttccct agccacgatg gtcggttcca taaagacgat gtcttagccg aaaaaatagc
    32761 tgtgatcatc aaaactgccg ttccgggaag ttgtacgatc acttccggta tccatgttga
    32821 ccatatctcc aatgcacaaa tcaacgcctc ttttccgatg tcagaagagt taggttacaa
    32881 gattttagat tggttggaaa aaatagattt ccatatcaaa gaaccagttt attataaaga
    32941 tggtgaaaaa acattatgaa agaagaggtt gtgaaatcag cgttatgacc tgagtatgag
    33001 agaaaaaaat ggaaaaatag cttctcatat tttgccgttt ttgagctaat accgagggac
    33061 agctgattga acgccgttta tcaagaggtt gtgataaagc cgcttcgttt cgagtagtaa
    33121 gaaaaatttt gtaaacatgg gtttccacat ttttgcataa atttgactta ctaccgagaa
    33181 actggctttt gaacaccgtt tatcaagagg ttgtgacacg tcttttgtca caacctcttt
    33241 tatttttgct tcactgtaac tgctacaata tcaccgattt cttttccgat gtttttacga
    33301 atatcttttc gtagtccaag gatgtagcag actgaaccat ctggattttt cacgcccata
    33361 ttcacgatac tgccttcata cggcatattg tcaaaagttg cttggacttt aactcttcca
    33421 gcaccgaatt cttttcttat atcatacgga aaaataatat atgccccgcc cttgtcgact
    33481 tcggaagcat agatttttga ttcaaaagaa taaatttccc tcattttatt tctcctattt
    33541 tttacatcta gtatagcata gcaaaaaaaa gaaggaactg tgacaaaagt cttgtcgcag
    33601 ttcctttttt cgaataaacg gtgttctcct gccaactcct cggtcacaag tcacaatagt
    33661 caacaaacat gagaagctgt tttttgaaat tgctccttgt gactcagagt taaacggcag
    33721 gctcacaacc tcttccttaa acggtgttca aaagcttgca tctacggcac taagcccaat
    33781 cagatgaaaa atatggagtg ctatttttat ctgattgttc ttactgcttg atgcagagca
    33841 gctttttcac aacctcctac atggtgttca aaatattatt cgccttttgc ttctttcact
    33901 aattttgcga atgcttgttg ttggaattca gcagctaagg ctttcgtacg ttgttcttgc
    33961 tctgctttgc tcatttttgg attttcagtc attgctttta ccataccgtc ttgaacataa
    34021 gcttttgctt cactttccat cgtactttgc tgtttcttca ctaattcagc taacttggct
    34081 gcttggtccg tacttaattc tacccatgtc tctgggattt tgaatgtatt cactcggctg
    34141 tcatattcgt gattgttatc agcaatattg aaccagaatt tatacatgtc gtttttgtat
    34201 tcccaataag tagttttcgt cactttttca gcatcgccgt ctgtcttttc tactttattt
    34261 gtttcgttat ccgtaccagt tgcttttggt ttttcttgtt tttcatttgt tcggtaaaga
    34321 tacacttttt cttttccatt ccctaaagct ttatacaaaa gcatatctgc tccttgggaa
    34381 tcagcactgg aaacaatttt ttctgttttt gtttccgtta ctttttccat tccaaagtga
    34441 ttggacaaat tagcgacgat caaacttagt gaagcgacga agattagtcc gaaaatcaaa
    34501 gataaaaagg tttgccatgt ttttttagcg aacacgttcg ttaaagcaaa agcaagcaag
    34561 ctgacaatca aaactccaat aatcatgcat tatcccccgc tttcattaga acaggttctt
    34621 ttttcttcaa taaaaaggca ataataaagc caagaatccc aaagatgatc gctacgataa
    34681 acgcggcatg gtaacctgac agtgtggcat tgattgcctg atctttataa gctaatggcg
    34741 tattatccaa tacagatttg cttgggatat ttgcttttgt cacattcgtc aacacactga
    34801 tcaaaatagc tgtaccaatc gagcttgcga tttgacggaa tgtattattt accgctgtcc
    34861 catgactgat caagttattt ggcaatgcat tcataccaga agtcgttacc ggcatcatga
    34921 ccatggagat cccaaacata cggatcgcat aaaggatcac gatatataaa actggagtcg
    34981 tttttgtcaa gaaagcaaat ggtgcagttg ccgctgtcaa aatgaacatc cctgtgatgg
    35041 ctaaacgttt cgcgccgtat ttatcgaaaa tcgcacccgt cacaggcatc atagccccca
    35101 tgatcaatgc acccggcaac aacatcaagc cagaatggaa tgctgattct ccacgaatat
    35161 tttgaatata caatggtacg accatttctg cccctaccat cgccatattt gttacaccag
    35221 ctaaaacagc tgcaatcgtg aatgtagatg atttgaatac acgaagttcc aagaatggat
    35281 gttccaaatg taattgacgc catgagaaca aggcgatgaa aatcacacca acaactaaga
    35341 aaccaattac tttcaagctt ccccagccgt catctcctac actagaaaat ccgtaaagta
    35401 aactaccgaa tccaatcgtt gataaaatag cggataaaat atcaatgcta ggatttgaca
    35461 atggaataac acttttcatt aggaagaatg ctaaaaccaa gacaagaaca acgattggca
    35521 gtaccatacc aaacaagtca cgccaatggt agttatcaat gatataacca gataatgtag
    35581 ggcctaaagc aggagcaagt ccaataacga tccctgacat ccccattgct gatcctcttt
    35641 tttctggcgg gaaaatagat agcatgatat tttgcaataa tggcatcgat actcccacgc
    35701 cgcaagcttg gatcaagcga ccgattagta atgtactaaa attaggtgct gtaaagcaag
    35761 taatcgttcc gatcaaaaag atcgtcatgg cagaaagata taaatttctt gaactgaatt
    35821 tattgatcag ccatgctgtt attgggatca tgatcccgtt gactagcaag aaaccagtcg
    35881 ttaaccattg cacatcagac gctgaaatat cgaaagcttt catcaatgta ggaaaagctg
    35941 tcgtcaataa ggtctgattc aaaacggtac aaaatgtacc aattaataag acaacaacta
    36001 aaagtgtccg attatatggt tttccgtaaa tatctacggg ttggttgtta ctcatatttc
    36061 tccttctttc tgaatttttt ctgaaactac tagaatactt tagtcctaaa ttcgctattt
    36121 gtcaacctac ttgacacata agttaatacg gatataatta agggaagcaa gaggacaaaa
    36181 ggagtgacaa aaatggttgg ggcttccctt aaacgtttgt tggaaaatga tcaattggtt
    36241 gttggcatca gtgaattaag tgatatcgta ggtgtgtctc cacgtcaatt aaggtattgg
    36301 gaacaaaagg gattcattcg ttcgatcgaa gctgatgcga attgtcctag aaaatatcgt
    36361 ttacctacag tcataaaagt agaactgatc aaaaaatatt tagatgacgg atttactttg
    36421 tcaaaagctg cagaaaaggc agaaatccgc caaaagaaaa tgcaccatgt ccgtaaagtt
    36481 ttttcaaaag caatcaaaga tatcgaattg atcgacgaac gctatacgat tttacaaatc
    36541 ggtaacttca aagaagagaa tgctaagatg tacatcattc atgacaacga gacagacgaa
    36601 ttaacttaca agctccttcc tgtcgatgaa aaagctgact acgaacagtt gttaaaagaa
    36661 ctctaaacgc cactaatctc ctaagtacca agctttttaa taagtagatg ctacataaat
    36721 acttgaaaaa aggcctgttt gaacataaga atatccgaac ggtgatctgc gtttctttca
    36781 tatagaaagg aattctccat cactgttcgg atattttttt atttttttcg gttactttct
    36841 tctaatacat ctgttagcca atttgcatat tctgtttccc gattgatcgc atgcgctaaa
    36901 atcaggtagt gtccgtaatt ttcatgtttt tctttttcag taggaaagat cgtttttttt
    36961 cgctctaata gatgaagtcg tttttcttca tgaaggtcac gctgctgctg aataatttct
    37021 actaatgccg gatcattcac gtctttgacg aaatacaatt tcagaacaaa ttcgtctcga
    37081 ttaaccggta gttcattggt tggtgtatga atccactctg ccaattgttc cttcccttct
    37141 tctgttaatt cataaacttt cttttccaat ttttcacctg tgatctctac ttgatgctga
    37201 atgattcctt gctcttccat ttttgcaagt tcaggataaa tctgactatg ttttgcttgc
    37261 caaaattctc ctatttcatt tttgaacgat tgattcaatt catatcctgt catcttcttc
    37321 ttacttaata accctaacaa aatatatcga agtgtatttt ttcttgccat cagttttccc
    37381 tccttgacaa atctttaaat ccattctatc ataaatgagt aatcgaaaac atgtagttta
    37441 ttacatgttt taatttataa ggagatgctt aaacatgaaa gaatttaaag aattggaaga
    37501 ttttattgga actcacttta tctatacgta tgacaatggt tgggaatatg aatggtatgc
    37561 caaaaatgac catactgttg attaccgtat ccatggcgga atggtcaaag gccgttgggt
    37621 aaaagaccaa gaagctgata tcgttaaatt gacggacggc gtattcaaaa tcacttggac
    37681 agagccaaca gggacagacg tggcattgga ctttatgcca aatgaaaaga aacttcacgg
    37741 aatgatcttt ttccctaaat gggttgaaga acatccagaa atcactgtta ctttccaaaa
    37801 cgaacatatc gcagaaatgg aagcagctcg tgaaaaatat gaaacttatc caaaattact
    37861 tgttcctgaa tttgctacga tcacttatat gggtgatgca ggtatagaca atgaagatgt
    37921 tattagcgaa gaaccatacg atggaatgcc agatgatatc cgtagcggaa aatacttcga
    37981 tgaaaattat aaacgagtaa aataaggttg tgaaagagag gttgtgaaat cagcgttctg
    38041 acctgagtat tagagaaaga aacggaaaaa tagttcctca tgttttgccg ttttttgagc
    38101 taataccgag ggtcagctga ttgaacaccg tttatcaaga ggttgtgaaa tcagcgttat
    38161 gctttgagta ataagaacgc tagttagaaa atagcttttc atattttcta ggctagtggg
    38221 cttattaccg agaagcagcc gtttgaacac cgtttatcaa gaggttgtga aatcagcgtt
    38281 ttgacctgag tattagagaa acaaatggaa aaatagttcc tcatattttg ccgttttttg
    38341 agctaatacc gagggtcagc tgattgaaca ccgtttatca aagattgtaa caactgcata
    38401 ttcacacagt taaattagtg acttcaaaat gaaataatgc tcccagcaaa aaattaggct
    38461 gggagcatta tttcgttatt ctgttcatca aaaatcagcg ttttgattcc atttcatcac
    38521 aagttcctcc tcatttactg cttcatcttt ttgctgtgtt tgaattgtaa aaccttgtga
    38581 aagatagaaa tgaatagctg attgattttt ttgatagact gtcaaagtca gtgttttatt
    38641 ggtttctttt atttttttca ttagttgcgt accgattctt tgttgccggt attccctttt
    38701 cacaaagatt cctgcgataa actgatcaat caccccagca aaaccaataa tttcttgatc
    38761 tatttctgct aagtaaatca ctgcatctgg taaagctttc ctaacagatt ctacttgatt
    38821 ctcccaataa gttgatggaa tgaattcatg agaatccaag ttagcatgca gccaaatcga
    38881 tagaacagat tccagttctt tggcagacaa ttttgttgtt gtgcggatca ttttttctct
    38941 ccttacttat tcattgattt taaaataaaa accatttttt tgaagctctt ttacaagttt
    39001 tcgcccctca aaaaagtata tcttctcttg ttttactttt ctaataccaa aagaaacagc
    39061 aactttagga tcatcagctg aaacgattcg ataagttcca ccaggcaatt gatccagctt
    39121 gactttccca tgatggtcgc tttgtactga aaacggcaca ccatttctag taagtgtttt
    39181 tttcttatgt ttcaaaatga atgaactacc atttaccggt ttccctgacg aatcactaat
    39241 aataaatgaa aaatcaagca cttttttact taatctatac tgatagatta gtcgtccgat
    39301 aaacactaac agtatgacta cagctattat cgttcctgca agaataaacc attgccgcat
    39361 ggttctttcc tgatttcctt ttccttcttc tttttttacc gattcgttat aaggaatccg
    39421 atgtcctgtc actagcaaac gatgcgtgtt gatcatatac ggcgtacatg taagcaaggt
    39481 aactaagtct tctccttctg aaatcgttag aacagacgtg tcatctggca gtaccacttg
    39541 tatattttta acttgataag ccaatttctc ccccaaaaca gtcaaaataa acgtgtctcc
    39601 gttttctaaa cgatctaatt cggtaaataa ccgtcgatta ggtaaaccgc tgtgagcaga
    39661 gatcactgaa tgcgtcgaat ttccaccaag cgggaaagag gttccttgca agacgcctgc
    39721 tccattttca aggatcctac cattgagccg atcataaagt gaaatattta attggatctt
    39781 aggtatattg atacttccga tcagccattc ctctaattgg ctggatgttg tactttcatt
    39841 tctgttcttt tcattgaaag gatcagtacc aggattgaat ccttggtcag ttaatcgttg
    39901 ttttttctcc tccattttct ttcgctgcac ttcaatttca gctgcggtcc gtttttgtac
    39961 ttgttcgata cgtttttgat caataaaact atttaaagaa tccacataaa atggatataa
    40021 agcgatcagt aaccctacga cgatacatat taataaaaac aaacgaaaca aaagtcgacg
    40081 agactgtttt tgcttactct gcttcacatg tgtcctccct tgcaagtttt tttgttcttt
    40141 cttttattat atgggttcag gaaaaaaata gaaaatgaaa gccgatgagg ttgtgaaaaa
    40201 gctgtcctgc atcaagtaat aagtacagcc caaaaaaata gctactcata ttttttgttg
    40261 ggttgggctt attaccgcag atgcaagctt ttgaacaccg tttaaggaag aggttgtgaa
    40321 aactgcgtta tgctttgagt aataagagcg ctagtcagaa aacagcttct catattttct
    40381 aggctagtga gcttattacc gagaagcagc tgattgaaca ccgtttaccg agaggtcatg
    40441 aaaaagctac tttgtttcga gtattaagaa aaattctgtt tcaaaacaag gagatgcaac
    40501 aaaacttatg tcgcatcctc tctccttaga actttcactt atgtttttct tgtaaaattc
    40561 ataaatattt tcatgaattt tctaatttct tgcctacctc tcgcatagga tatatgatga
    40621 atttatctaa agtttgcaaa ggagttcgac aattgaaaaa atatggccca tttttagcaa
    40681 tttttttcat tgctttggca tttctcatgc ctcatttatt cacacataaa ctaattttgg
    40741 gtgctgatgc cccattccat tacaatcggt tctatgatgc cgcgcaacaa attaaatatg
    40801 gggatttcca ttattttcct tccctttatg gattccagca aagtggcagg atcgtgaatg
    40861 ccgtttacgg cccatttttc gcttatttac aaggattgat cgtgttgctt gcaggtagct
    40921 ggtttcatta tcagattctt tcaaatttca ttctttatct tatttcagga ttttctatgc
    40981 tgattttact gagatatgtc aatgttcgaa attgggtaag tgtaccagta gccgttttat
    41041 tcatgagtac gtattctatt caatactggt ctatcaacca aggattcact agctggggga
    41101 cttgttttta tcctttatgt atgatcccta ttgctgattt cgtccttcat aaaaagttcc
    41161 ctgttatcaa agtcgctgtc tcagtgggac ttatgacaca gatccattta ctgtcttcga
    41221 tcatgttagc tttgatgtat atcccttttt atctttatta cttttttaac cagactgaaa
    41281 aaggaaaagc actgcttcaa ctgattaagt cggtcttgct ttattttgtt ctgacagcaa
    41341 atatttgggc aggattagct attgtttaca gtgggaataa gattcttcct ccctttgtta
    41401 acagagaaat gagtcaaaag gcgatagatc tggatggcag ttattggctc cattacccta
    41461 aggcctttcc tatattgata ttatcaggtt ttttgctttt tattttcttt caacaaaaga
    41521 acaccttatt ccaaaaaatc gtgttgtaca caagtcttgt tttctttttt ctttctacta
    41581 gcttgattcc atggacgaca ttgatcgaaa aaaatgtgcc ttttattagt ttgatccagt
    41641 ttccatttcg atttttcgtg ccatttacgc tattgcttct actttacctt gccctttcac
    41701 tgaaccagtg gtctgaacaa aagtggttcc gtcttttaag tatatttggc attcttattt
    41761 tttcagcaca gacgatccag aacttatacc agcatctgga aaagtgggaa aatgaaacat
    41821 ttgtcagtcg acatacttat ctattcgata cgccggaaga agcaaggaaa acattcttca
    41881 gcaaagatat gtctaccagc ttgttcgttt tccaaaaaac cacacctgac tatctgccaa
    41941 tctataagga gacaaaagac aataaatacg accggtataa cgaagaaata ctagaaaacg
    42001 aaacaacttt tataaaaaca catccaggcg gtcttttgac aatcaagtgg aaaaacactg
    42061 gggacaaaac tgttcatatc cctgttatcg tctacgatcg gactgtgcta cagcaaaatg
    42121 gaaaaacatt gactgattat gaagtaacag atatcgggac cccaatcgtg aagcagaaaa
    42181 aaggaatcaa cgagctcacg cttcattatc aaacgccaat ctatttctat ttcattcttt
    42241 ctcttacttt gattggctgg tttaccttgc tatgtttatt tatttatcat agatacaagc
    42301 ttttacgtgc ttaggcaaaa gggataaata catccgatca aaaaactctc caaacggtat
    42361 aatcgtttgg agagttttat tttaatcagt ttttttatgt tatatgttat attttttatt
    42421 cgttcacatc taattcgatt tccaccccgt agtgatcact gatgattgga ctatcgatcc
    42481 catcaaaaat ccgttgatat tttttaacct gcattccttc agggacgaaa ataaaatcta
    42541 tacgcaattt ttctgtattt tgttcccaac catctatttt cttttccaca gtagctgctt
    42601 ttcctttttc attcgcgaca acatacgaat cttgaagaag aaggcaactt tctcttacag
    42661 tataataacc ttgagagtct actggattat tgaaatcacc taggaaaaaa agtggtacgc
    42721 gagtttccaa aaagtagttt tccaagtttt tccactcaaa ataaaagcca ctttttttat
    42781 ctgtccacca agaaaagtgg cagctcgaga caaaaatgtc ctgatcatct atctttgttt
    42841 tcccaatcaa gatcttgcgt gtctgataat cttctttctt ttgagattgg gagacggtaa
    42901 ggacctgact ttccaatgtt tttttcgtaa gcagtgcatt tccttcttca tatttatcat
    42961 aaccgatgtg gctcatttcc caactccaaa aatactcttt tccgtgttcc tttaaaagct
    43021 gaactaaacg gtaggcaaaa ttatctttac ggataggtac ttgattcttt acctgacaga
    43081 acttcattag cttcgcaggt tctacttctt cagattccag taattggttg atttcttgta
    43141 aagcaatcac ttcataatct tctaacagga tcttatctgc taattcttgc aattttttct
    43201 ctggttctgg ctcaagccag ctatgcgtgt tgagtgtcat gaatttcatt ggttttctcc
    43261 ttgtcttcat ttcgaaacaa aagggttgtg acaaaatttt tgtcacaacc tctccagcaa
    43321 atagtgttct cctgctaact cctcagtaat aagctcaatg gaccaggagc tattttttgt
    43381 ttggctgttt ttatcacttg gtgcagggca gctttttcac gacctctcca ctaaacagta
    43441 ttcaaactgc tgcttctcgg taataagccc aaccaaacga aaaataggag gagctatttt
    43501 tgtttggctg ttcttatgac ttgatgcagg acagcttttt cacaacctct ccttacattt
    43561 taatggagcc gatcacttta ccagctgtca ctgtacctaa ttgcttgatc actacttctt
    43621 ctactttatc gtttgtaaat acgatcatga tcgttggatc tttttcttcg ttttggatca
    43681 catcaagatc catctcagct aatttcgttc ctgttcctac ttcttggcct tctttgacag
    43741 caatctcaaa agctggctga ttcatttgga ctgtatcgat tcccatatgg atcaaaactt
    43801 cgataccatc tttcgtttcc agcccaattg catgtttgct agggaaaata cttgtcactt
    43861 ttccagcgat cggcgcaaca acttctcttg atgttggaat aacagcaaag ccgtcaccca
    43921 tcattttttg tgagaatacc ggatctttta cttcagataa aggaatcact tctccagaag
    43981 ctgcggaaac tagttcttgt tctataccta agtattgctt actacctgct tgtccatttc
    44041 ctgtagaaac catttctggc tctggaatct ctgctcctga ttgcagcaga tcttcgatat
    44101 ctgatttcaa gacatctgct tttggtccat aaactgcttg gactccattg tctttgacga
    44161 tcaatcccat cgcccctgct cgtttccagg cttcttcagt ccctactttt tctcgatcat
    44221 ttacacttac gcgtaaccga gtcatacaag catcgacatc tttgatattt tcttttcctc
    44281 ccagcaaatg aatgatctgg accacttgct gatctgctgt tcctgttccg ctgttagata
    44341 caacggtttc ttcactgtca ttgtcgtaat ttccgttacg tcccggtgta gcaaagttga
    44401 attttttgat caagaaattt gctaagaaat acgtaacaac accaaaagcg atcacacaca
    44461 acacgaagtt gataagatct ccacctagac ccgctttgat agctaacggt gtccgagtca
    44521 gcaattcgat attcccaaat gaatggaccc gcaatggcaa gatatctgcc atcgcaaatg
    44581 cagccccttg gatcactgaa taaatgacat ataatggcac agcagcaaac ataaacatga
    44641 actccaatgg ttctgttaca cctgttagaa agacagccaa cgctgctgaa aaatacattg
    44701 atttatatct tgctttctta tcaggatcga cattacgata catcgcaaaa gccattccca
    44761 tcaagatacc tgaagaacca atcatttgtc ctactttgaa acgagccggc gtccagttat
    44821 gcaacacaaa ctcatattgc gacatatccc ctgcaccttt aagattcact aaatcagtgg
    44881 cccaagccaa ccataacgga tcttgcccaa atacttgtgt ccccgcctgt gcgcctgata
    44941 aaatctcata cgatccgcca agctgtgtat agttgatagg aatcgtcagc atatgatgca
    45001 atccaaatgg caatagtaaa cgttctaatg ttccaaataa aaacggtgct agaatcggtg
    45061 cgctttcttg tgattctgca atccataaac caaaattatt gattccagcc tgtacgtttg
    45121 gccacaccac agcaagaatc aatgccacga ttgttgacca taagataaca acaaacggaa
    45181 caaagcgctt tccattgaaa aatgacaaag catccggcaa ttttctataa ttatagtact
    45241 tattataagc cattgccccg acaaatccag cgatgatccc tacgaatact cccatattca
    45301 aagctggtgc ttccaataca cttgtaaaaa atcctttaac catgatcttc gtaccgaaca
    45361 gagtgtgggt aaatgcatct tcattcgcaa gcatgtcact tgtgacacca aagatagccc
    45421 cagtgatccg attgattaaa ataaatgaga tcccagctgc aaatgctccg cctgctcttt
    45481 cttttgccca acttcctcct attgctaaag caaataaaat atgaagatta ccaataattg
    45541 cccaaccaat attttcaatt actccgcccg ttgtgacaag cggcgtccaa tcaggattga
    45601 tcaagggaat tgatttccct atactgatca tcaacccagc tgccggcata actgctacca
    45661 caaccattaa agcctttcca aatttttgcc aaaactcaaa actaaatatc tttttcattc
    45721 cgtttagctc ctttacgcaa tcggtttcgt taaacgtatt atagcgtgcg ttttcttttt
    45781 tgtaaatgct ttatttttta tttttctgtc tataccattc tataaaacct ataatacgtg
    45841 tgttcccttc tataattttc tcctaaaaat ttattttttt aagctttttt ctatgaaata
    45901 ttttcttttg tctattgact tgtgcaatcg gttgcgttta ttatgaacgt ataaaacagc
    45961 tcattaggag gagaaaaatg aaacagatca aacgactatt tgctattgat ccatggaaaa
    46021 tcagcacgac acaattagat aaaaaaaatc ttcgactgca agaatcttta acaagtattg
    46081 gaaacggtta tatggggatg cgcgggaact ttgaagaaac ttactcaggg gaccaccatc
    46141 aaggtactta tttggcgggt gtctggtatc ccgataagac acgtgtcggc tggtggaaaa
    46201 atggttatcc agaatacttt ggaaaagtga tcaacgcctt gaactttatc gcgatagata
    46261 tttcgatcaa cgatcagctg atcgacttgg cttctttgga accagaagac ttttactggg
    46321 aattagatat gaaaaatggt gtattatctc gtacattcac aatcactact tctactaata
    46381 aagtacgcat ttccttcgaa cgttttctga gcattgtcaa aaaagaatca gcttatatct
    46441 atttaactat agaaatgctt gaaggtagcg gaacagtcaa actcgtatca aaattggatg
    46501 gaaatgttca aaacgaagac agcaactatg atgaacattt ttggaatgag attgatcgtg
    46561 gaactataga agaaatcggc tatttgacga ctaaaactat tccaaacaac tttgggattg
    46621 aacaatttac tgttactgca gcaatgcgcc atttcgttaa tggaaaatca gtcatccctg
    46681 actatcacga agatacttta gcattacatg gttgcttgtc tgcagaatta tctcaaggtg
    46741 agacatggtc agtggaaaaa gaaattatcg ttcttactag tagagatatt ccagaagcgc
    46801 agcaagcaga aaaagctgtc tcagaattaa cgaaccagac aaacaattat atccaagcaa
    46861 aagaagagca aacagcggct tgggcagaac gatggaagct tgccgatgtg gtcatcgaag
    46921 gagacgaaga agcacaacaa ggtattcgat tcaatttgtt ccaactattc tctacttact
    46981 acggcgaaga tgaacgattg aatatcggcc ccaaaggatt cacaggagaa aaatacggcg
    47041 gagccactta ctgggataca gaagcgtatg ctgtccctct ttatcttgct ttagccaaac
    47101 ctgaagtaac taagaatctg ctgaaatatc ggcataatca actgccgcaa gcaatccaca
    47161 acgctcagca acaaggatta aaaggcgcgc tttatcctat ggtcaccttc actggtgtcg
    47221 aatgccataa cgagtgggaa atcacattcg aggagattca ccgaaatggc gcgattgcct
    47281 atgcaatcta caattatacg aattacaccg gcgatacgga atatttgaaa aatgaaggac
    47341 tggaagtttt agcagaaatc gcacgcttct gggcagaccg tgtccatttt tcaaaacgag
    47401 caaataaata catgatccac ggtgtgaccg gcccaaacga atatgagaac aatatcaata
    47461 acaattggta tacaaatacg atcgcagctt gggtcttgcg ttacacgcga gaaaattatt
    47521 taacttatca aaaagaagct gctatcacta tcagcgaaga agaactagcc aaatggaaag
    47581 atatcgcaga gaacatgtac ttcccagttg acgaagaatt aggaatcttt gtccagcatg
    47641 acacattctt agataaagat ctcatgcctg tatccgatat tcccacaagc gatcttcctc
    47701 tgaatcagca ttggtcttgg gacaaaatcc ttagatcttg cttcatcaag caggcagatg
    47761 tactacaggg gatttatttc ttcaatgata cttttacaaa agaagaaaag caaaaaaact
    47821 ttgatttcta tgaaccaatg actgtccatg agtcttccct ttcgccaagt atccattcga
    47881 ttttagcagc agaattagga atggaaagca aagctgtaga aatgtaccag cgaacagctc
    47941 gcttggattt ggataattac aataacgaca cagaagacgg cttgcacatc acttccatga
    48001 ctggtagctg gttgtcgatt gtccaaggat ttgctcaaat gaaaactgcc catgatcagt
    48061 tgagattcgc accatttctg ccaaaaactt ggaaagctta ttctttccat atcaattatc
    48121 gcggacgttt attgttcatc tcagtcacag atgaacaagt tactttcgag ttattagaag
    48181 gttttcctct agaaatttac gtctatgaac agtcttattc tttagcggat cgtctagaaa
    48241 taacgctaag aaaggaggaa gcaaatgttt aaaggggtat tgtttgattt agacggtgtc
    48301 attaccgata ctgctgaatt ccattatcgt gcttggcgaa agcttggtca ggaaatcggc
    48361 attacgatcg accacacatt caacgaacag ctaaaaggcg tcagccgaga agactcactc
    48421 tctttgcttt tagcttatgg cggaaaagaa cactcttttt caaaggaaga atttgcagaa
    48481 ttagcgaaaa gaaaaaacga ttattattta gagatgatcc agacgatcga accaaaagat
    48541 gtcttccccg gggtcgtgcc tttactggat tctttaaaag aagaaaaaat caagattgct
    48601 cttgcttctg caagtaaaaa cgggccattc ctacttgaaa aaatgggtct tacaccttac
    48661 tttgatgcta ttgccgaccc ggcagaagca acaaacggaa aacctgcgcc agatatcttc
    48721 ctccttgcag caaaagctgt cggactaaaa gcagaagaat gtatcgggat cgaagacgcg
    48781 caagccggta tccaagccat cctctcaagt ggcgctcaac caattggcgt gggacgtgct
    48841 gaagaccttg gagaggagat cccgcttgtc cctgatacga cttttcttac gatcgattac
    48901 ttgaaagaaa agtgtcatga ccatggataa tccagagatc tccgtcgtac cagttccaga
    48961 gactgaaata aagaaaatca cattgaagaa cgactactta gaagtcgttc ttctcaatta
    49021 cggtgcccgc ctgcaccaaa tcttcgcacc agacaaagaa ggaaaatcag aaaatgtttt
    49081 attgtcttac gatcgtttcg aagatgttct aacagacaaa agcttttttg gagcaactgt
    49141 cggacctgta gccggaagga tccgggatgc ttcttggggt gagcatagat tggagaaaaa
    49201 ctgcggatca caccatatcc acggtggcac gaaaggctgg tctttccaat tttgggaagt
    49261 agaagttttc aaaactccat catcaatagg tgtggtcttt tacttaaaag atgaattttc
    49321 tacctatcca ggaccaatca ctgctacgat cacttaccgt ttaacaaaaa acgaattaga
    49381 gatgacaacc tctgctagta gccttgttga aaccatctgc aatccgacca accatgccta
    49441 cttcaacctt tcaggaaatg gaacaagaga catctatgaa catcagttaa cggtcttttt
    49501 agatggtgtg cttgaattag atcaagaaaa actgcctact ggaaattgga caaaaaaaga
    49561 aaaactgcca atcgacttta gaaaatcccc aacactgcaa gagatccttg catgttatcc
    49621 agatggacta gatgatgtat ttcttttgca tcatcctagg ctttccaaaa cttccttaca
    49681 attattcgag aagcattctg gtcgccaaat gactatagca acttctaata aaagtatggt
    49741 attattttct acgactggat tcgaagctga tttctctgtc aatggaaaac agatgcattc
    49801 aaactacgga ttagcaatcg aaccgcaaga aatccctgat atcgttcatt tccccaaatt
    49861 tggatcgatc aacctccatc ctggtcaaga acggatcagt cagacgattt atcggttctc
    49921 tgcacaataa caagagaaag gtgtttttat catggctatt accgtaaaag atgtggcgaa
    49981 aaaagccggt gttgccacct cgaccgtctc tcgtgtcatc aacgatcatc ctagtatttc
    50041 cgagacaaca aagaagaaag tccgaaaaat tatggatgaa ctaggttatg ttcctaacat
    50101 gactgcccgc aatctaggaa aacgcatttc cagtgccatc ggcgtaatct taccccctct
    50161 tgattcaaaa gagcggatcg gtaacccatt ttatcttgag atcatggaag caatcaacga
    50221 agaagcgcgg aattatgata tgactacagc aatcgctact gctaaaaatt ttgaattgct
    50281 gttagagaac gtccagcgta tgcatttaca aaaacaagta gatggtttta ttctagtgta
    50341 ttcagatagt caagatcctg tcattgatta tttataccaa aaccacatcc ctttcacttt
    50401 gatcggacag ccttatcatc atgagaacga aatcgtttat gtagataatg ataaccagct
    50461 tctaggtaaa caagcaacag aatttttgat ccagaatgga cataagaata tcctgtttgt
    50521 gacaaacacg actcatgaaa gtttgtattt cgaacgatat ttcggttatc aaaaagcaat
    50581 gatgatggct ggtcttcctg cgttatccag tgtagtacta gagcatccgg aggattatct
    50641 gtcctttgag aaaacgttga aagaaaacga agccacagca ttggtggtca ttgacgatat
    50701 cttcgccctt cgcacgatgc agttggcgca aatgtacggc tatgtcattc cagagacact
    50761 ctccgtcatt agcttcaaca actcgatctt ctcaacatta acacaccctt accttacaag
    50821 tatcgatatc gatatctccg aattaggacg tgtcgctatg caaaagctgc gcgaattgat
    50881 ccatgaagaa ttgagcagtg gcgtacgttt ggtcatccct catcgcttga tcaaaagaga
    50941 gacagtcgtt cctttagaaa acccatacta aatagctgac tcactaaata cgctgaccat
    51001 ttatcctatg gtcagctttt tatgtaaaag aagatactac ttctaacaca ttgtttatct
    51061 gtaaaaaaga gcgtgggaca aaagtaaaga acacttttgt ctcacgctct aaacacgtat
    51121 aaacggcgga agcagaagca actccttcgg aaataagctg aaattcacaa aaatttaaaa
    51181 agcaattttc ttgagtttct tcttatttct tggagttaaa cgcttctgtc ccagcctctt
    51241 ttttccttcc ttcaattttt tacacgtcga cgattcaata aaaaaccgcc taatattgta
    51301 aagacaggtg caagaatcga gaaaaagacg aatggcaagt aatgcaacgc gctaactttc
    51361 atcgttccca taatgaatgt gccgctgact ccccacggta cgagagaatt gacggtcgca
    51421 cctgaatccg ctagtgttcg tgtcaaatag gtttttgata agccaacgtc atcaaaagaa
    51481 cgcttgaatg tttctcctgg aagaatgatg gacagatact gttctccaac gatcagattg
    51541 attccgatac agctcaaggc agtaaacccg actaaacgag aaggtcggtc catcttttct
    51601 tttaattctt gaacgatggt atcaacgatc agatatttga tcagtaaccc acctaatgcc
    51661 aatgcaagta aaatcaaact agcagaacct aacatactca tgatcccgcc tcgagaaagc
    51721 agttcatcta gctttggatc aggtgtattt gcgacataac cattcatcaa gatgtctgct
    51781 gcttttccga ccgtcaaact tttctcatgg ataaaagaaa gaacgagagc agctgtcgat
    51841 cccgctagta atgtagggat agccggaatt tttttgattg cccccccaaa taaaataact
    51901 agtggaatca atgtccaagg agagatccaa aagctcgctt tcatgttttg gatcatttca
    51961 ttgatactag ctagatcggc tgaatgactg ttatgtccta atatcgtata aaaaatcaac
    52021 gaaaggacga atgctgggaa atccgtataa cgcataccag cgatatgttc gaatagatta
    52081 actcctccaa tacccgctgc caagttggtc gtatctgata aaggcgagca gttattccct
    52141 aaaaaggcac cggaaacgac tgcgcctgta gtcattgcat cattgaatcc aagtaaatga
    52201 ccgatcccca aaaaagcaat tccaatcgtc gaaatcgttg taaatgaact gccgacagtc
    52261 actccgacaa ttgcacaaac aagaaatgct gtcggtaaaa agaaacgcac agaaacaagt
    52321 ttaaatccat aaaccataat cgtagggatg gttccagata aaatccatga agcaactaag
    52381 acaccaatca aaagaaaaat caggattgga atgatccctg gagtgattcc tttgatgatc
    52441 ccgtcatgga tctcatccca tgtgaacttt ttaaatctgc cataaaacaa caaacacatg
    52501 aaagctgcaa ggatcgggat atgtggcgag agctggaatc cgatgatcag tactcctagg
    52561 agtgccaaca acacgatcaa tacagcgata ctttctttta aagaaatatt tttattcatc
    52621 gtcgatctcc tatttcaaat gaaaaggccc tgccatgaaa aaactattca cttggcagga
    52681 cctcctacta aatcagcgac aaaacgtcgg ctgaaagact tatttttttt atttttcttc
    52741 taaacttttt tctacctcaa tcccccaaga atctttcttg aagtgattga tgatcaatgg
    52801 aataacgaac atgaccactg tgatagctgc tactaataat gcatcactca atgtcgctgt
    52861 acctaatgta gcaaatacga caacgatgat agagaagatc agcatgcagc tgactaccca
    52921 agctaatccg tttccgtttt taccaattcg gtaaggacgt tcgaattctg gatgtttctt
    52981 acgcatttta agcaatgaaa cagcaatcaa aatgtacacg atacaataaa taacagttgt
    53041 agcatttgta agtgtcaaga atacagcact tacgtcatcc attacaccat aaagcaaagc
    53101 aaataatgaa ataacgattg attgtgttag aacgacattt ctagatacgc cgtatttgtt
    53161 ttctttatgg aatccgaatt ttggtggaag gaatccttca cgagctactt ggatgattgt
    53221 ttttgatggt ccagtaaccc aagctgataa ttgtaaaagc acaccaataa ataccatgaa
    53281 gctgaagata tttccgatca cttctggtaa gcctaggatt tgacagtcaa tcaagatagg
    53341 ctgcgtgata tttgctaatt ccatttttcc atctggtacg acatttgcta caagcatcgc
    53401 attgatgaca ttcaataaaa ctaatccgat caaagcgata aatacgcctt ttgtataatt
    53461 ttttgttgcg tctttcaaac gaggcatgta aacagaactc atttcgatcc caacaaaaat
    53521 aaatgtgata ccagctaagt atttcaatga atcaatatgt tccaagtcag ggaaagcttt
    53581 tgaccatgaa aagtcaccta gatagccgtt tgagactagt ccgactttga aggctgccaa
    53641 aacacctaaa acgaacatga tgactactgg gatatatacc cctagccata caccgatgtt
    53701 cccgccgact tttgccatat cgaatttcaa gttcaagatc gtgatgatcc agtagataac
    53761 taaaatacaa cctaaaacga accagtgatt attgccaagt tctacattac caaatgtgtt
    53821 tcctaacaat ggtcctaaag tagaagcgac cattaccata cctgggaaca tttgcaccca
    53881 taatagccat gcaacgacaa atccccattt gctgcccagt gctgttttta cccataactg
    53941 aggaccgcct tcttgaggaa gcatcgtgga aagctcaccg gaaaccattg atatcggacc
    54001 ggcaaaaaac aaaactgcaa aaattgaata aaaaatcaat gtccaaccga ctgcggctaa
    54061 cgtaggaata ctccgaaccg ttgcacacag tgccattgtc agaccgataa aggtcccaag
    54121 acccatttta cttacttcag atttactcac cgttgacact ctccttgatt ttttggataa
    54181 aataacggtg tctttgaagt agtttcagca ataaacgaaa caattcagcg aatatttgag
    54241 gggattcttt gagtctattg cttgaagggt ttcttcaaag acaacctcaa aacaaaggga
    54301 tcgaagagct taggtctccc cttgctcttc agatccaaac atgcgtcaga aacagttagt
    54361 attcaagctg ttttctaaca tgattacctt gcttattttg cttcgcttgc caataattgg
    54421 tctgcgtaga ttttttccaa tttttcttga agagctgctt tgattttttc agcatattct
    54481 tcaaagtgtt cttctttgtt catgtatggt gtcattacgc tggcacgcaa tacagttact
    54541 tttccggcac ggttccattc ttcatctgag aatcctagtt ggttcacaaa ttgtaatggg
    54601 ctgttgccat agtctgggat agcaaagtca gtatgtgaag ttaagaactc attaccgtaa
    54661 atgcttcctt tcacgtatga agaataatca tatacgtcat ggttcaattt attcattgca
    54721 actaagtcat cgttgccttt ttctttgaat acatagtcta ccatattgaa atctggataa
    54781 gttaatggat gaacttcgat ttctttgtct cctactttaa atgacaagtc attcaagaag
    54841 ttgtagaaac gatgtgcacc ttcgattgat gcacccatta atttaccata tcctgtgaca
    54901 ttcaaaggta agacatggtg tgctgcccaa acgctggctg ctgttgcccc tgcttttgaa
    54961 ccttccaaga tataagcccc aagtaatgct gggatatctg cgcctttttc aaatacatat
    55021 gtcgcaaagt aagagataac gtctctcatg cggatatctt tgatgacgat accaccggca
    55081 gaatatggaa catagcccat tttatgtggg tcaatcgtta cagattctgc ttcttcgatt
    55141 gctttatatg cactgtgtac ttcttctaag atataatctt tgttttctgt aaagacattg
    55201 tatttgtaat gaacatcttt taaatcttca aatggaatga agttgttgtc ttcgtctaag
    55261 aaaatcgcac ggccgtatcc gccataagca gcatctacgt gtaagtagaa gtaaataccg
    55321 tctttttcta atacgcgacg caatgcaacg attttatcga taccgtcgat tgctccttct
    55381 tctgttgatc ctacaacacc gactacacct aggattggtg ttttttcagc tgctagacca
    55441 cgaacgattt tttcaagttc gttgatgtcc atgcgatagt tgtggtcaac tggcactgga
    55501 atcacttggt ctaaaccgat accgatgatg tcggcagctt ttagccatga atagtgttta
    55561 gtttgtggaa ccaaccattt tcctaatttt tctaaatgtt ttccgctacg tgctgagtga
    55621 gctttgattt cgtcgatttt ttctgggacg ctgtctaaca gattcatgat ttcttctgtt
    55681 gacaagttca ttaattccca atcgcttttt ccagcaacta gttcaggtgt cacttctttc
    55741 attgcaagcg gcaatgattt gatgttgcgg gcataccaaa gtccttctaa gtttgctaaa
    55801 gaaccatcag caacgatgtg gccccagcca tctttatagc tcattaattt tgcaaattcc
    55861 attcctactt cttcttccat ttggcttgtt gcaggagaag attcataggc aacgttatta
    55921 ccattccata gcatagcaaa attgtacgct agtaaagatg gcatcaatgt ttcagagttc
    55981 atatgacccc agtaacgtcc ggcattatgc caaggaacgg aatgggtacg catccgcgct
    56041 gaaatttctg atagaacatc ctttgtttta ttcacagtgt gttcaaaact agcactgctt
    56101 ttttcttctg gcgtaataat aggcatgtct tgaggcatgt agttttgacg ccagcctaaa
    56161 tgttcatcca ccaattcatt caacaaagct ttatagattt ggccgttctc agctttgtcc
    56221 ccaatgaata gggcatttag gtttagatct ttcgacaatg attcactcat agtgaaaccc
    56281 tcctaaaaaa atatttaaat tttgtcgagc cctacattac acctgttctt ctattctgta
    56341 aactaagttt tggcctgatt tttaatcata ttttaatatg tatcaattat ccgaattgtt
    56401 cacacttttg aaataattaa aaaaaacgag ggaatttaca aatgtaaact ccctcgcaaa
    56461 gattgtatta acgacaacca gcgttctatt ttaggatggg tttcaaaatt ggaatgttgt
    56521 aatagtattt ccctttttca aggtaaaaac ttactaaatt ctaatcaatt actttggcca
    56581 aaaagtaatt tttcttacct tttcgaataa ccacaaactt accatcgaat tcatcggatg
    56641 gatttatgac aaaatttaaa tcggtaacgc ggtcaccgtt aatacttata gctccgtttg
    56701 aaacatcttc acgtgcttga cgtttagatg gttcaatttt tgtggaaaca agcagttcca
    56761 cgatattttc aggtgtgctt gaaatttcaa cgttcggcat tttaccgaat ccttgcgcga
    56821 tttcttccgc atttagttct ttaatatttc cagagaacaa tgcttgcgtt attttttgcg
    56881 cttctttcaa gtcttcttca ctatgcacaa atcttgtaac ttcttctgcc aaacggcgtt
    56941 gtgcttcacg tttctcaggt tctgtctcta cttttttcgc taagtcttcg atttcttctt
    57001 gagacaagaa agtaaagaat ttcaagtatt tcactacatc gcgatcatct tgattcaacc
    57061 agaattggta gaactcaaat ggtgaagttt tcttcggatc aagccaaacg gcaccgcctg
    57121 cagtttttcc gaatttcgtt ccgtcagcct tcagcatcaa tgggatcgtt aaaccgaacg
    57181 cttctacttc tggtccttct aattttcgga ttaggtctaa tccagctgtg atatttcccc
    57241 attggtctgc tccaccgatt tgcaattgca catcgtatgt tttatgcaga tgcaaaaagt
    57301 cgatagattg cagaatttgg taagtaaact ctgtaaatga gattcctact tccaaacggc
    57361 tggcaacgat atcttttgct aacatcgtgt tgatgttgaa gtttttaccg tagtctctca
    57421 aaaattcaag taaagagatt tttgacaacc agtcgtagtt gttcacaaat gtgatatttg
    57481 catctttacc aaacaatttt ctcatttgat tggaaagtgc gtccacgtta tgttgcactt
    57541 gttccatcgt ttgcaacaca cgttctgttt tgcggccgct tgggtcgccg atcgttcctg
    57601 ttccgccgcc aatcagaata tatggatgat ggcctgccaa ttggaatctt ttcatcatca
    57661 taaatggaat caaatgtccg atatgcatcg aatcaccagt tggatcaacg ccgcaataaa
    57721 gtgaaatgct cttttcttca acgagctcgc gaagtctttc ttcgtttgtt tgctgattaa
    57781 tggcgtcgcg ccaagttaat tcatcaataa tattcatttt actgacctcc gtgtagtttt
    57841 tttatgaaag caggaacctg ctagcatgtg taattaatta tttcttcagg ataggatagg
    57901 aaataaaaaa agtcccttgg catataagcc aagggacgaa atctcgcggt accacccaat
    57961 ttgtatgtct agatatccaa catacctctc gaacatttaa cgctgtcgcg tggcaatcac
    58021 tgccaaagac tcctgaaatg taattcgctt taaagcatgt actggtttgc agcgaccacc
    58081 agctttctga aacattggct ttaacaacta cttcgtttcg atcttcgtct tgttattatt
    58141 aatatgtgtc tatcatagag cgcaactttt tctttgtcaa cagaaaaagg atattttttt
    58201 taaattcctc attttttctt ttttgaatcc gctctaattc cgaacatttt attttcaatt
    58261 attgttaaaa gaaataactt tttctgtatt ttttctgcaa tttatcgaga agtcacttta
    58321 ctttgcctct tattctttct tcgttacaat aaggaatggg acaagtaaaa aaatgaaaaa
    58381 gcaagggacg aggtatatgg aaaaaaagca aaaaattatt ttaggagcca cttctgcttt
    58441 aactatcgtt attttaattc taagtttgtt ttttgtcatc cgatcatcta caaaaaaaga
    58501 accaatcatt cctgaaaaaa aaataacaga aaaagcaaaa gaaccgacag atgataagca
    58561 acaactagtt actcaagcaa aaaatctggc tcaaagctac gattatgacc aagcgattgc
    58621 gttattggaa aaagatagta agaacggatc tactgaaaca caacagcttc ttcaaacact
    58681 aaaaaaggag aaagaacaac tgatcgactg gtctgatcct actcagatct ctcatgtctt
    58741 tttccacagc ttgatcgtgg acccgcaaaa agccttccac tctcaacagg cacaaggata
    58801 caaagattat atggtgactg ttgaagaatt caaccgttcg atcgaacaac tttatcaaaa
    58861 tgattttgtt ttagttaatt taaatgattt gatccaaaaa gacgaacaag gaaatctttc
    58921 ctttaacggg cttgcgctac ctgaaggaaa aaaaccattg gttctttcac aagatgatgt
    58981 cagctattat gaatatatgg ataattccgg ttttcctagc cgattggtcg tatcaaataa
    59041 caatgaaatc aaaaatgtct ataaagagaa agataaagaa gaaatcggtg actttgacat
    59101 ggttcctctg atcgatgcct ttgtcaagaa acatcctgat ttttcttatc gaggcgcaaa
    59161 aggtaccttg gccctgacag gttacaacgg agttttaggt tatcgaacat caaaatctca
    59221 atatggagac aatgaaaaaa caaaaaaaga gattaaagaa gctaaaaaag tggcggaaca
    59281 gttgaagaaa gacggctgga gctttgcttc ccatacatgg ggtcacttga atatgactca
    59341 agcttcacta gaagatgtaa aaaaagacaa tgaattgtgg cagcaagaag ttgcacctat
    59401 cctaggaaaa acaaatattc tgatttatcc atttggtgca gacatcagcg actggcagcc
    59461 ttattcagca gaaaatcaaa aatttgctta cttgaagagt caaggtttcg atattttctg
    59521 taatgtcgat gcttctacac cagcttgggg acaattaggt acggatttct atcgaaatgc
    59581 tcggatcaat atcgatggca ttcgttttca atctgattta gacggaaaaa atcctattct
    59641 tgatccattc atcaatgtcc atgatgtcta tgacaaagaa gcacgcgaat ctgcttgatt
    59701 ttacgtcatt tatgaaccaa aaaagccgaa ctgacatttt atcgtcagtt tcggcttttt
    59761 tatgctttat ttcttcttcg gtgcgattcc cttgatcagg acttctgtcc attctgctgt
    59821 atatggaagc agttttgcgt atatctccgg cttagaaatt ccttctacca ttgcttgaaa
    59881 atagaggatc aagaactcat ccgaatattt caaatccact tttccttctt tccgccctcg
    59941 atggaaaaga tcaagcatga tactgaaact ttcttgcgta tacgcatcca tcgcgtcaga
    60001 aaattctttt ttttcttttt ttgtatagta tgtcatcaaa tcgatataaa attgtttgtt
    60061 cactcgattg agataacgga ttttgttttc actcatggca atcagtgttt cttcaaatgg
    60121 aagattttcc gcaatcaacg ctttcgcttc ttgccctaaa tcattcataa atgcaaaaaa
    60181 tacttcataa atcagccctt ccttgctgcc aaaatattta aaaatcgtcg ctttacttac
    60241 ctgagcgttc tcagctacct tatccattgt cactccagcg gttcctatgt ctgtgttcat
    60301 caagtcaaaa gctgcttgta aaatctcttt ctttttctct tgtgctctct tttcaaaacc
    60361 gttcatttgt agagcctccc gtaattttaa acttttataa tatatatagt tcaaatttat
    60421 tctgaggttt attataacta tctattgaga aagtatcaag ctagcaattc cttgtttatg
    60481 aattcattga cagaaattta taaaaagaat attatgtact aaaataataa aaaaagtttt
    60541 ataaaaaggt ataaaacctt tttattaaac tatttaatct tgtttagtat atatttttag
    60601 aaaaaggagt ggggttttta tggcagaaat cgtcaaagtc acacatttga aaaagagctt
    60661 tggtcgtttc gaagctttaa aagatgtctc tttcaccgta cattcaggtg aagtcgttgg
    60721 atttatcgga ccaaatggtt ctggaaaatc cacgacgatt cggacacttt tagggattat
    60781 aaaaaaagac ggcggaacgg caacgatttt tgggcaagat gtatgggaag atagtttatc
    60841 tatccatgaa cgtctttctt atgttccagg cgatatctct ttgtggggaa atttgactgg
    60901 aggagcgatc atcgatctct tcatgaagct tcatggaaaa gggagtgctc aaaaaaaaga
    60961 tgaactgatt caacgtttct ctcttgatcc taagaaaaaa gcaaaaaact attcaaaagg
    61021 aaatcgccaa aaagtgggct tgattgctgc tttggctgtt gattcggatc tctatttatt
    61081 tgatgagcct acttctggtt tagatccttt gatggaaacg attttccaag aagaagttga
    61141 aaaattgaaa gaacagggca aatccatcct actttcttct catattctca gtgaagtcga
    61201 gcgactagcg gataaagtcg tgatcatacg cgaaggaaca gtcgtagaag aaggaacact
    61261 cgatgaattg cgtcatttga ctcgttcgac catcactata gaaacagaag cagagaatag
    61321 tcacttagca caattgaacg gtgtccatga ctttgtgcaa aaaggtcacc tcgctcaatt
    61381 tgctgctgat aacgaagctt tcgatgtgat tttagctgaa gcgtccaaat taggtgtgaa
    61441 aaaatttgaa tctattccac cgactcttga ggatttattc atgagacatt atgaaggatg
    61501 aggaggaagc gaaatgactg ataaattttc acgctggtcc accctttttt ctcaaaacct
    61561 tagccgtgac tggaaaaaga tcctgatatg gctagtcggt gtcactgctt tttctggtgg
    61621 ttttgtccct gcgtttgaag aattgacttc cggacaagga ttagctggat tatacgaaac
    61681 cttgcaaaac ccggcaatga ttgcaatggt cggaccaact ccagcttcga atgctgctga
    61741 gtatacttta ggagcgatgt atgcaaatga aatgttgctt ttttgcagtt tgtttgctgc
    61801 tgtgatctct gttttacacg tggtgagcca aacgagaaaa aaagaagaac ttggtctgat
    61861 tgaacttatt cgttcctttc gagtcggacg tcaagcaaac tctttagctg tcatcgctga
    61921 aagtattctg atcaatttgc ttttagcaat atcgatcagc tgtacgatga tttttttcca
    61981 agcagattcg atcactgcag aaggtgcttg gctttttggg ctatctgtag gtagtatggg
    62041 gattttgggg agtgccgttg cactgtttgc tgcacaactg atgccaagtt cagccagttc
    62101 gaatggtctg tctttagcgt ttttaggtgg actttacctt ctacgagcaa taacagatgt
    62161 agctgattcc tccctttcga tgtggaatcc tttaggctgg tcttatttga cctatccatt
    62221 tacaactaac aactggattc ctttgcttta tttgtttttg ttcagtctaa tcttgataat
    62281 cagttcattt gttttagagg aacatcgaga tttgaacacc ggctatatcc ccgaacgtga
    62341 aggaaaaccg tatgctaaac gttctctttt atccttgcgt ggtttattat tctatctaaa
    62401 taaagggatg acaatcagtt ggcttatcac ttttggtttg atgggggcgg catacggctc
    62461 tatctacggg aatatgcaac agtttttaga aagtaacgac ttaatgaaac agatgtttat
    62521 ccaatctggc gtctcaattg aaaaaaactt tacaagcacg atcatggtcg tcttgatttg
    62581 tctagctgct attttaccaa ttgctagcat caatcgttta tttacagaag aaacccacgg
    62641 acgtatgaat cagctgttcg caacaaaaat ttctaggaac caactttatt ggaccaatat
    62701 gttactagct attctatttg ctatattggc aatcctgatt agttctggtg cactagggct
    62761 ttctgctttg gcttcgatga aggatgcgaa agacatggat ctattgatgt ttcttaaagc
    62821 cgggttcaat ttcctgcctg ccgttttagt gtttatcgga attagtggct ttctattcgg
    62881 atggcttcct cgaatgggta aacttgctta tatctactta gcttactcct ttgcactcaa
    62941 ttactttggc ggaattttgg atttaccaga atggttttcc aaaacagctg tccctagctg
    63001 ggttccacgg atgccaaacg attcttttga tctttccgtg ttccttacgt tatcatgtat
    63061 cagccttgca ctgttcgtcc ttggttctat tggttatagg cgaagagact tactagaagg
    63121 ataataaaaa agcatgtaca atttcatgct agcctccaaa tctgggtcaa aaatccaaca
    63181 tttggaggtc atttttttat ttacaagtct tctaactgct ctttatcaat gatccagact
    63241 tctcgattct ttctttttac ataaccattc tcctctaata atttaaattt tctagataaa
    63301 gtttcggggg tcgtaccgat aaatgcagca agttctttca ttttcattgg caggaaaaag
    63361 tgatccttcc ctgtcgcttt agctaagtca agcagataga cagcgactcg ttcttctact
    63421 ttttccatca tcaaaaaagt tgtctgctgc tgtacttttg ttaatttctc aacgttgatt
    63481 gctaaaagtt tcagactaag ggcgggataa tgcagtaaga gttccgagaa atctttttgt
    63541 ctcaatatgc aaatttccgt tttttctagt gcttcgccaa ataaagactc atttggaacg
    63601 cctagcaact ggctctctcc ctcatatccg cccggctcta ttactcgtaa aagctgttcc
    63661 tttccgctag cagataattg atacactttc atactgccgg aggccacgat cactaattga
    63721 ggttctccat tcggcgcaag aatctgttct cctttttcca catagtgatg tttgacaagt
    63781 gcgtgtactt tttcttgatc ttgctgtact aagtgattga acaaaggaac aaatgaaaca
    63841 cataaatgat catgactcat aatctctcct ccttctaaac agctatttct aataaagaaa
    63901 cataacttcc cttattccaa ctgataattt tcttttatca tagcataaac gtcaagcgaa
    63961 ccagatacca gcaaatagag tggatttctt tttgtccctt gctttatttt ccttcttttg
    64021 agaccttaag taattgattc ctatcaattt tttaaatatt tactttttga ataatcaaaa
    64081 acaaaccttc actcgcaagc cgagagtaag gtttgttttt cgcattgctt atttcgtgtt
    64141 gtctattgaa atttttccgc acctagcttt tgatatagtg gtgctagttc ttgtaaggtt
    64201 tcagcggtta tttgctcaac gtgttcgatt tcttgcggat agaagaatag tttacccatt
    64261 tgaaaggttc ggtctctcat cgcttttttc aactcttctt gtttctttgt atcggacaaa
    64321 tagtcagaca aagctaagtg gtcgtcaagt tcatgttctg gttgcggcca tatgtaataa
    64381 tccttgatat cgactgtttt gctccactct ttcagtatcg caagcaactg gttatacgct
    64441 gcaatgctac catatcgttc ttcgctttta taatgttggt acatcaaata gatttggtat
    64501 tgtttttgat tcaacaaaac tgctagacag gcattctcgt cttgacgaga ttcgcttcgg
    64561 tacgcgctcc aaaaatgact gcgtaaattc cagccattcg tccagctttc gattttaggt
    64621 ttgctcatgc cgtacatatc aggcaaaagt gcagctgtct ggagctgaat ctctttccat
    64681 ttttgccagt gttctttata ttctttcttg atttgttcaa tcttatctgt tggatggctc
    64741 tctttgagct gtttgaatgc gtagattggt cgatcaaaca actgataatc tgattttcca
    64801 agttccattg tttcgcctct ttccgttatc ttttgtattc tacctgaatt ttgcgatatg
    64861 gacaaataga ggttgtgaaa aagctgttct gcatcaagtc ataagaacag ccaaacaaaa
    64921 aactgcgatg agcatcaccg cagttttatt gaacttaagg ttgattgatc acatctaaaa
    64981 gcgttgaaaa taaaatggca ctagaagcag ctcctgggtc gatatgtccg attgcacgct
    65041 ctcctagata ggaagctcgt ccttttttag ctaccaagac tttggtgtgt tcttttgcct
    65101 ggtcaatgat ttcttttgtt aaattccctg ccttcaatgc ctctgtgact ggcagccaaa
    65161 catctaacat cgttttatcg tcagcttctg cctttcctcg gctttgaata cccgctgttc
    65221 cctgctcgat cacttcgcct aatttttctt gagaatcaat agaatcgacc tcttttgtcg
    65281 ctttcatcat gttcatgaat gcagatccgt ataacggacc agaagcccca ccgactttcg
    65341 atatcatcgc catagagaat actttgaatg tatcgctgat tgtttcagga tgatcggctt
    65401 gaaaagcttc ttcataagct gaaacccctc ttcccatgtt atttccatgg tctccatcgc
    65461 caatcggggt atctagatca ctgagatatt gtttgttctg attgattgcc tctgcaaatt
    65521 gcgtgagcca ttcttggatt tcttgaacgg ttagtttcat attatcaccc ttttatcttg
    65581 agatttttta ccatgaaatc gtctgtacat tgctttctaa agaatctttc cattgtgtct
    65641 ccgtcaagtc gatcagtgtt agagaaagac cttgcatatc tagtgaggtc ataaaattac
    65701 cgacttttcg gaaaactaca ttgacgcctc tttcttccaa cagagctaaa acatcattca
    65761 tgaacacgaa ctgttccatc aaaggcgtcc cgcccattcc gttgactagt acacctgctt
    65821 cttttggtaa attcgagtaa tcatctaaaa tcttagtaac aagttccttt gccagtactt
    65881 tagaaggctg catcttttct cgacgatagc ctggctcacc gtggatacca acaccatact
    65941 cgatctcatc ttctcctagt tcgaatcctg gtttccctac ttctggtaca gttgctgctc
    66001 gaagcgctag accgatcgtc ttagttgcct gtacgacctt ttctcccagt tctttcaact
    66061 cagctaggga agcaccgttt cttgctgcat cgcccacgat tttgtgtaca aggacagtac
    66121 ctgcaactcc gcgttttcca gcagtatacg tactattttc aacggcgata tcatcatcta
    66181 cgatcacact ttctacttca atatcgtcca tagcagctaa ttcctttgcc atatcgaagt
    66241 tcaatgcatc ccctgtataa ttcttaacga tcagcaaaac tccttttcca ctatccgctt
    66301 ccttaatagc cgtctgaatc tgatctggag ttggagaagt aaacacatca ccaagcaccg
    66361 ctgcacttaa catcccttcc cctacaaacc cggcatggga tggttcatgt ccgcttcctc
    66421 caccagaaac caaaccaact tgttccgctt tctgattctt ggctacaaca cgggaagatt
    66481 ctacacgatg aacaagttct ggatagcttt ttactaatcc ttccagcatt tcttcgacaa
    66541 tattgcttgg atcattcatg atttttttca atttattccc atcctttctt tccggcattt
    66601 tgtgcttctg ttaagatttg ctgagtatca tctgttatac cggcagtgat ggctgctgca
    66661 aaagctcctt ccactaaagg cgcatccacc agatgataac gttgctgctg ctcttcttcc
    66721 aacatgtctt ttgccagttc cgagttcaat accgcgctac cgatatccgc aaaaacaaaa
    66781 taactagctg catcgcttga ttcattgact gcttctacga ttttcatcgg gtctgatccg
    66841 atctcgccat ttattccgcc gagagaaaaa atttgcacct cttcggattt ttgcatctgc
    66901 tcaatcattt ctttaatacc atctgttact ttttgactat gagaaataag caaaatactt
    66961 tttttcacaa gaatcttcct ttcttttaca tagttgctaa cgattggttt cgctttcatt
    67021 aatgaacaat tcaagtatat aataaatttc taattttggg aagatatgtg tagtgcttct
    67081 tgaagtttgt tatactttaa ttaaaagaga ggagtgatcg aatgattaca acaacaacga
    67141 acagtgtaga gggaaaagcc gtagtagctt ataaagggat cgtttttggt gaggtcatta
    67201 ctgggatcca tgcatttaaa gacttggagg cggggttgcg aaatatcttt ggcggccgct
    67261 caaaaagcta tgaaaacgaa ctaatgggtg caagagaaga agcacttgcc gaaatggccg
    67321 aacgtgcaaa agcattagga gctgatgcga tcattggcgt aaaaatggac tatgaagtat
    67381 taggcagcga caatggcatg ctgatggtca catgtagtgg aacagctgtc tccacgagat
    67441 aagtagggga catctgtttt aataactaaa gagagatggg acatgtagtg gcactcaaga
    67501 attagatttt tcagtctaac ttcgaggtac tctaccttcc catcctctct tttttatttc
    67561 tttttgcgga tgattccgta agctgctgcg actaacacac ccgcccctgc taaggaataa
    67621 ctgacagaag ctgttttttc atttgttttt ggcaaagtct gacttgttga tttggatggc
    67681 tctgtcgttt gctgaactgt ttctgtcgtg cttccagagg tcactgattt ttctcctgcc
    67741 gaatgcaaaa tatcttttat tcgttggaaa ctagctgcta agtcagcttt ttttgcttca
    67801 aaatcattag caactgtttg tgcttcgctg atctttccgt ggattgcttg actggccgct
    67861 gtgatatccg cttgagtttg acttagcttt ccagaagctg tttgagcaac ctgatttaat
    67921 tgctgattga attcttttgc ttgttggatc ttcccgtcaa gatctgtttg gacagcttcc
    67981 gcttttgctt tcgcttcggc aagctttttc tcaagttgtg cacggtcatt tcccacttta
    68041 tttttaagat cagctaaatc ttgatcgatt gctgcttggt cttctttcaa tttgtttttt
    68101 gcagcttcta atgcttcttc gagttctttt cttttatttt ccaagtcttg caaatgatcg
    68161 atttggtcag aagaggtaga actatttatt gatgtatctt ctgaagatga aatcgtttct
    68221 cctgaattat ctgtcggtac attggaagtc atttctgttt ccgaggcacc gttttcagca
    68281 ccagaagagc ttatagttgt tgaaggttct gtttgagcag gttcagaagt ttccgcaaaa
    68341 gctgtctgtc ccaataagaa aatactggaa acagcaattg gccagattaa cggcttcatt
    68401 ctttttttca tcacttttcc catccttact actttttaac taatttctta ttataagcaa
    68461 aaatgtttgg aagaaccata aacacaacag gctgtttgtc taaaaaacgg tgcatctttt
    68521 atttttttgt acgttccggg cttttccatg tcccgcttcc ataacctaaa atcgttttga
    68581 ctgctggaat gaaagtcgtc acgatcgtta tcgcgttcac tatccagaac aaaagcatat
    68641 atagtggtgc gaaaagaagg tatttcaatt ttcttcgtcg atcatctaca atcagcgaag
    68701 ctagcaactg gaaaaatcct gcgaccattt caaagcagat gaacacaaaa ctgaatgtaa
    68761 aagtaatata aatctgttcg tagtttcctt gatatacata atgcccgatt aatccagcaa
    68821 aaagaactac ggaaatacaa aagaatattg accagacaat actcaatgtt tgatcaatga
    68881 agatgatcgt tcttccaata tgtttaaaag gatggagcat cacttttttg aaattcgtca
    68941 gccagacttc tgttccacct tttgcccaac gttttctttg tcgatatagc atcttcaacg
    69001 tcacaggtac ttccatgaaa aagataatct caggagcaaa aacagataac cagtcattta
    69061 actgatgatc ccaagcaata ctgatgtctt ctgtcgctcg atcttgtctg aatccgccca
    69121 catcgatcaa ggcttctttt cgatacatcg tatttgctcc actgtacgca taaagaccac
    69181 cgaaaacagc tgactgtgtt cgtttaatga tgcccacgat acttgagaat tcaaccgttt
    69241 gagattttgc aatcaatttg gtacgatttt ggacatccat atttgcagtg accgcagcaa
    69301 tatgtcttgc accagggcga atgaaataat tcacatacct aatcagtgca tctggctccg
    69361 gtaccgtgtc tgcatcattg cttaaaatca atttcccttt tgcaaaggca agtccgatat
    69421 taaacgcgtg ggcttttcct ttattttttt cgatccgaac cacacgaaga ttagcgtatt
    69481 ttttcatcag tctagctaat atttctggtg tttggtccgt tgaaccatcg tctgtcacta
    69541 atacctcata gttgtgataa ttcaatttcg tcatcaaata ctcgattgta tcttcgatca
    69601 caatttcttc attatgggct ggcaccatga tcgtgatgaa cggttcgaca ctcaagggaa
    69661 catctaccca ttctttttgc ttatgtttgt ataaaaacac ataacaccag acaccaataa
    69721 accatacaaa tccgcctaaa atcggataag aaacgagaaa aaatacataa tagacaaaca
    69781 agtatctaag aataaattac ctgtatgggt gattgagatc atttcagttc caccccattt
    69841 tctttataca aatctgcaat gaatgtcgta tctagatttt tctcttcgga gacagaataa
    69901 aaacggaccg tttctctctc ttcttttgtt ccaaaacgtt ccgtatacac ttcttcaagt
    69961 aattcttgtc tttttttcaa tttttcatct tcatattcaa aagatttatt tacttttttg
    70021 tatagatttc gattgttcca cctagtcaat acaaatgagg taatcaggaa actgaaaaaa
    70081 atcaatacaa aaaagccgat taagaaatac agcattttct tttctgataa aaaagcgtac
    70141 aaggaaaaat ccttggctct ttctggtctc gttaatggga aaaagaccca tataaaagga
    70201 agaacaacga tcatccatcc taaaagagcc actaacgttt gccaaatctt taaccagtaa
    70261 ttaccttttg aaaaatactt atctttcacc aaaattgatg caacttcttc tcttgttact
    70321 tttttcatat cctcacctca cgtttttttg ttacgacgta ggagtttacc atacatttca
    70381 tttcttttca cataaagtgt tcctaaattt ttcctaagaa aaagaacaaa acatttctca
    70441 tcagcaaatg aaaaatattt tgttcttttt atcagttgtt ctttttattt tttatctctt
    70501 aattatgttg ctctttccaa tacgacaatg cttttcattc ctctagatat cgccgtgtac
    70561 atttgtctgc gtcttctttg ctcatctcca tcgttttcaa atggatataa gattacatga
    70621 tcaaattcca gtccttttgc tagactcaaa ggaatgattt ggcagttttc taatccatat
    70681 gaattttttt taatcgattc ttgtagcgtt tgggtttcct cttctgtttg ggtgataact
    70741 gccgcttttt ctccttttag agaaaccaaa atctcagcta gtattttcaa caattcttca
    70801 gaggatcgaa aagagcggta ttcgggttct tttccttgcg gtcggatcga tacgatgtcg
    70861 atcttctctt gatccacagc ataggttttg aaaagctctg tgattgctcc gctagaacgg
    70921 taactatttc taagatcata ccgatggatc ggcagatggt agtcgtcgaa acaacgcata
    70981 atatcagcaa aagtagtgga agaattaaag attgcctgat tctcgtcacc tactaaagta
    71041 aagtgagttt ttggaaataa ttcgatcaat agacctaatt gtgccagggt ataatcttgt
    71101 acttcatcaa tcaatagata gcgaaatgct ggtacattta ccttttcaac aagtaaatga
    71161 cgaatagcta atataatgac tgcttcatca acagtacgtg tgggttgcca agcataagct
    71221 gagccatatc gtttttcata aattctttca aacaactgat gctcatccac ccatgccatt
    71281 tcttcgattt gtctggttat ttttcgatat tttttttgta aaagtttcct tgcataagct
    71341 acaatacttt gttccgaatc atcagaaatc aatttaccga ataattccaa ctgcctatct
    71401 tctgtcaacg agagtacctg attctgcaca gcaggacttt ttgcttgttt caaaagatga
    71461 ttcttccatt cgctcatcag tgcctttttc gttgcttgaa gcctatcgat caatttagga
    71521 tgagcaggaa ctttctcata tattttttcg atcgtttttt tagaaaggac tgtttttcca
    71581 tttttagtaa gtccacggat aaaattcgga tgatctaaaa acaaaggatc tgattgtttt
    71641 agattatcga taaacttttt gctacgcaaa cgttctgtct gctccgatac attttctccc
    71701 gtgatacgtt tgaaataagc ctcttcacct tctatttcct ctgctgaaag ctgtgaaacc
    71761 agctgtatga tggttagatt cagtggattt ctttcaccta atgatggtag gacattcgaa
    71821 atatagtcga taaaccgatg attaggtgat agaatcagca agtcatcgga tgtcatcgta
    71881 tctcgatata aatagagcag atacgcaata cgctgcatga tagcagaggt tttaccactt
    71941 cctgctacgc catttaccaa gatattttga cttttcacat cccgaatgat tgtgttttgt
    72001 tccgattgga tcgtagcagt gatagccttc atttcattgg tctcatcttg gcctaagact
    72061 tctaataata cgtcatcttg aatagctaca gacgtatcaa aaaacttgac caaacgatct
    72121 ctttccaaaa gaaattgtcg tctttcttta ataaccgtat cgattttatt ttgacgaacc
    72181 gtataagaag atggaccaat catgttattg taaaaaagtt cggcaattgg tgaacgccaa
    72241 tcataaacaa gattttcatc tgcatcatct gtgaaatttg ctgtaccgat ataaaaggat
    72301 tcttttggct cattatccag aaagtctaca acgacttttc caaagtaggg acttttcaat
    72361 aaacgttcga tctttgctag ctgattttct gcgtttttct gtttgatatt catctgatcg
    72421 atttctctat ttttcatttc aatcatcgaa aaagtatcca ggttatctaa gtaactgtca
    72481 aaattcaatc gaagctcgcc agccatttct ttcaacgact ggtttccttc tatttttgcc
    72541 gtatccatcg attcccgcag ttcgtttttt ttatcaatta gcttttgata cacgttcttc
    72601 agatatgctt cttctaactg tttttgtgtg ttgaccatac acattctcct ctttttttaa
    72661 agtggtggaa ccgattagtt tacctctttt aagagtcgaa tgcaagcttt ttaacgagta
    72721 tcatttgtta ggatatatca gttcttcagg tcggacagtt tgatataaag aaaatgcacc
    72781 atcaaggttt tttacgtggt agccagactg cttcaagatt ctttccgcta gatagctacg
    72841 taatccgcta tgacagctga cgatatattc ttgttggctg tctaattcat ttaatcggtc
    72901 ccgaagttca ttcaaaggga tatgtttggc ttccttgaat ttgccctttt ccaattcttc
    72961 tgctgttcgc acatctaata aaatttttcc ttttgctagt tctgtcggca attcatgcca
    73021 ttgtacggtt tcactaagcc cttctgcaat gttcattgct gcatatccta gcatgtttac
    73081 tggatctttg gctgagccaa atggcggtgc atacgtcaat tctaattccg gtaaatcgaa
    73141 gatcgtcaaa tgccctttga ttgctgttgc taaaatatcg atccgtttat ccacaccttt
    73201 tgccccaaca ccttgtgcac cataaatctc gccagtctct ggatggaaga tcaacttcag
    73261 taaaatatct gttgcttctg gataatagct tgcatgatct tttcctgata catgaatgac
    73321 ttcaaaagac agttcgtttt ctttggctgt tctttcactt aaaccggtgg atgcggcagt
    73381 cattccaaac gttcgtataa ttgctgttcc aataccgcct tgatttcttc gagaaattcc
    73441 ggaaatcgtg tccgcaactt gtctaccttg acgattggct ggtgaagcaa gagaaataag
    73501 tgcatctttt ccagtgattt gctgttttac aacgatagca tcccccaccg cataaatatc
    73561 aggaatattc gtttgataac gttcgtctac taggataccc ccgcgtatgc ctaattcgat
    73621 tccagcggtc actgctagct ggttatcagg acgaacacca atggataaaa taactaaatc
    73681 agcagggatg gtacgcccat catccagtcg caatcgtttc ccgtggtctt caaatccagc
    73741 gacagcatga gaagtaatca cttctactcc ttgatgagac aattcttttt ctataaaagc
    73801 tgccatttct tcatccagtg ttggaagaat atgtggtgct tgttcgatca ccatgacttc
    73861 taaacctctt ctttttaggt tttctgccat ttccaatccg atgaatcctg caccaacgat
    73921 cacggcacgc tttggctgtt tttctaatgc atgcatcact ttatcgatat ctggtacatt
    73981 tctaatagaa aaaacattgt cggcttctgt tatccctgga atcgatggaa caactggttt
    74041 tgcaccagga gataaaacca atgcatcgta ctgttcaatg tgtttttgac cctcatgtac
    74101 gacttctacc gttttgtttt cgggaaagat ggctgtcact tcatgatttg gacgcacatc
    74161 taaacaaaaa cgagcggata atgattctgg cgtttggaca agaagatttt cccgttcact
    74221 gatttctccc gaaagataat aaggcaagcc gcaatttgca aaagaaacat acggtccttt
    74281 ttcaaaaaca acgatttctg catcttccat taatcggcga agccgtgtag cagcagacat
    74341 tccacctgct acacctccga caataacgat tttcatctta tttccctcct actgttcgat
    74401 caaaccattg attcatgcca ccacgtacat tcactacatc ataaccagat ttctttaatt
    74461 ctttagctgc ttgtttactc ctcatccctg attgacaaat cacatagacg gtcttttctt
    74521 tgtctccatc atatctgttt atactttgta atggaacatt tttagctcct tttatatgcc
    74581 cgccccggta ttctgcaggt gttctaacat caagtaacgc tggattttcc aaaagctttt
    74641 cctttaattc tgggactgtt atcgatttta ttgattgaaa tagcaaattc attttctgca
    74701 cctcatttac atttttataa atacctcatg gggtatatta aacaggagct ttattaatat
    74761 gtcaaccaga atgactcttc atttttttca ctacttttta tatttgcata aaataccgat
    74821 aaggggtatt atctaattat aaaaatagag gagaatgtgt tatggagatt gataataaaa
    74881 atatcctgaa ccgtttgaaa cgtacagagg gacaaattcg tggtatccaa aaaatgatcg
    74941 aagacgaaaa agaatgtatg gatgtcatca ctcaattaag cgctgttcgt tctagtattg
    75001 atcgcgtaat gggcatgatc gttgcagata atctgaaaaa ttgctttgag aaacctgata
    75061 gtaatccaga agaacaagcg gctaaactag aacaagcgat caagatgatt attaaaaagt
    75121 aaaggaatga tttcaatgaa agtcgttttc catattgatg aattagaaaa atggtcagaa
    75181 acagggaaaa acgtaaaaaa cctgataaaa gcttctccag aaacgacgat tgtcgtatct
    75241 gtaaatggca tcgcgattac cggttatcta gactctgcca atgcagaatt cttagatttg
    75301 caaggagtcg tttttcacgc ttgcgccaat gctatgcgtg ctaatcatat ttcagaaagt
    75361 tctttacctg aacaagtaat cgttgtacca gcaggtgtcc ttgatttagt tgaactgcaa
    75421 tcccaaggtt atgcttatat caagccttag tcagtagagg ttgcgacaga agtgatcagc
    75481 ttcaagaaat ttcggcttat ttttcgaaga agctacttct ggaaaaccgt gaatcaaagg
    75541 ttgtgaaaac gctgtcctgc atcaagtaat aagaacagcc aaacaaaaat agcttctcat
    75601 atttttcgtt tggttgggct tattaccgca gatgcaagct tttgaacacc gtttaaggaa
    75661 gaggttgtga aatcagcggt ttgacctaag tattagagaa aaaaacggga aaatagctcc
    75721 tcatattttg ccgttttttg agctaatacc gaaggtcagc tgattgaaca ccgtttaagg
    75781 aagagattgt gaaaaagtcg cttagtttcg agtattaaga aaaattttgt gaaaatggat
    75841 ttctacattt ttgcataaat ttggcttaat accgagaaac tggcttttga ataccgttta
    75901 aggaagaggt tgtaaaatca gcggtttgac ctgagtatta gagaaacaaa cggaaaaata
    75961 gctgctcata ttttgccgtt ttttgagcta ataccgaagg tcagctgatt gaacaccgtt
    76021 taccaagagg ttgtgagcct accatttaac tccgaatcac aagaaataat ttcaaaaaca
    76081 acttctcctg tttttggttt ggttggctta ttatcgagaa gtagctactg ggagtctatt
    76141 tatcaaaaaa aggaatgcga cataggtcac accccttttt tattttcttt gaaacatctt
    76201 tcggtactcg acagcataca agacaaaaga ttgtaaaaga ccgtaagtga tgacggcaaa
    76261 aatgactctc acccacagcg ttggtaattc tgggaaaagc agcaaaagga tcatgccaac
    76321 atagatgaca gcagtcgcta ctttgccata ccacttcgct ccacttaatt tgatattttt
    76381 agatagcccg attaatccag ctacaaacat aaagccttct tttatcaaga atagaccaaa
    76441 caacagccaa agccacggtc gataccatgc catagacaaa atcagaaaaa gttgtgtcaa
    76501 tttatctgca aacggatcaa gcaccttccc taagtctgtt acctgattat atctccgagc
    76561 aatgaccccg tctagataat cagacaaacc tgagacaaca atgaccgctc ctgctatata
    76621 aaatgactgt ctcagcacaa aataaagata aatcggcagc aaaaaaatac ggaatatcga
    76681 taaaagattc ggaatcgttt gtatttcttt acgccagtct gtgcttaatt tcattttatc
    76741 gccctacctc ctcagcactc ttttcttttt attttagctt acacggggaa taaaaccaat
    76801 gatttcactg tttttatttt tctttacagc cactgatatt atgagctaat ttgagataat
    76861 cacttgctgt tttcatcgtt tactcttctt tcttcctgtc gtttgcgcat ttcttttact
    76921 agtactttat gagctttttt tgatcctcgc tctaggtcac gctgccgttt acgtgtttta
    76981 taatcgataa acagactgtc taaatcgtaa cctgtcgggt acagatcttc tgctgaacgc
    77041 accagacgaa cacgctgacg aggcacgtgg attatttttt tgtctaaata tacatcaatc
    77101 gtatcttcac ctatatcttc ataaactaac gcttcttttt ggtattcatt gacaaatatc
    77161 cgatcacctt ttgcaaagag ctggcgttct tttttattct ccttcctatt tgtaactgct
    77221 gtcttaggaa gaaattcttt tttctgtgtc cgataggctt tttccgccaa gtaacgctct
    77281 gcttgatgga tcaatttcat cgacatatcc attttatgag caatccacaa agcttggctt
    77341 tctcctgttt ctccaacccg caactgatat ttaggacgaa gtgcttcccg atcgaaagcc
    77401 atagcagccg gcacaaaatc ttcatgatct ccggcgaatt tcttgatttc tccataatgg
    77461 gtcgtggcaa tgatcaatgc cccttgctca tacatagatt ccatgattgc gatcgctagt
    77521 gcagcccctt catttggctc tgttccactg ccaatctcat ctaaaagtac caacgtattc
    77581 ctttttatct ttttcaaaat agcagcgatg ttttgcatat gaccagaaaa tgtactcaac
    77641 gcattttcta aattttggtg gtcaccgaca tcgacgaaaa tctcatcaaa gacagcaagc
    77701 tctgtccctt cttttgccgg aacttgcata ccgaacatcg ccatcaaagt caataaaccg
    77761 actgtcttca acacgactgt tttgcctccg gcatttgctc cggtgataat caagcctcga
    77821 tagtctttac cgatttcaac atccaatggt accgcatgct caggtaaaaa tggatgtctg
    77881 ccttgcttga taatgatatg ttccgattta ttgattctag gtgtgcttcc attgatttct
    77941 cggctaaatt ttgcccgagc aaagatgata tctaacaccg tgatcgtttc gatcaataaa
    78001 tcgatcactt cttcgttttc tgcgatagca ccagtcaatg ccgccaggac ttgatattct
    78061 tcactgattt cttcagcttt taacaactga tagtgttcat tcaatttaga aatcacagtt
    78121 ggttcaataa aaaccgtcgt tcttttattt gattcatcga taatcgttcc atctatttta
    78181 tttttatagc tggctttgat aggaatcgta tagtattcgc ctttttgaac aatcattgct
    78241 tcttggatca tttctttgtt tttcggatga cgcaagaatt tcagtaattt tgactggatt
    78301 tctttttcga ttagttgtaa ttgtttgcgt actttacgca aattacgtga cgcgtcgtca
    78361 ctgactttat tattttttat tttctggtca atcaactctt ctacatttat caaatcagga
    78421 agatgtttgc tataagcaaa caacaagggt gtctggtatt gattcttatc aaaaaacttt
    78481 gtgatcatcc gactgcttct aagaaaatcc gcatattcga tcaaatcggt tggctgcaga
    78541 atcaaccctt ttttgacttg ttctgtcagt gcatcgatcc tcggcaaccc cataaatgga
    78601 acatgctgac tgctttctaa gatcaagcgt gcttctcttg tttcttcttg acgatccaag
    78661 acagtttgta ggtttgtcga gaccgtcata tcgctgattc gttttttgct atagtttcca
    78721 attgctcttg cttggatttc ctttttgatc cgatcaaatt gtaattgact aatcatttcg
    78781 ttgtccatat tctctccttt tcttgaagag atacctgtcc caaaataaat aagggcctag
    78841 agataaataa aaggatagaa cacagcttaa aaaagtcatg tcctatcctt attagaatac
    78901 aaagatattg ttttcctgac aggtaccgtt tcataagcac accaaataaa ctttgtttat
    78961 tggctattcg acttactgat tagataccag ctacactcac aaaacatacc ccattcttct
    79021 aggtcttatt tattttccac ttaactatag caagacacat tttggttgtc aaactttagt
    79081 atgttttata gatttacacc attaatgaat agacacctac cgcgataatt gctccgatca
    79141 ttggacctgc gattggtacc catgaatatg cccaatctga agttccttta ttagctattg
    79201 gcaataccgc atgtgctaat cgaggaccaa agtcccgagc agggttgatt gcatatcctg
    79261 ttggccctcc taatgaaaga ccgatggcta agatcaatcc acctacagcg aatacgttcg
    79321 ttccaggtgc aaattcgttt tgtccaaaag ctagcaaccc aagaactaat acaaatgtac
    79381 caattaattc tgtgatcaca tttgctggat aattccgtat agcaggacct gttgcaaacg
    79441 ttcctaaaat cgcgctttca tctttcgttg cattccaatg tggcaaataa gaaagccaaa
    79501 caagaatggc ccctgcaaaa gctcctaaaa cttgcgcaat gatgaaagga acgacaagat
    79561 tccaactaat tgcccctgtc atcgccatag ctagagagac tgcaggattc aaatgagctg
    79621 ggcccatgaa tccagaaata tagacagcca ttgttacagc gagtccccaa ccaaatgcga
    79681 tgacaatcca gccagaagcc tgtgctttac ttttgtttaa attcacagcc gcacacacac
    79741 catctccaag caaaactagg atcagtgtcc caataaactc tcccatgatt tgagtcatat
    79801 cactgttcat ttatttctcc ccctttagtt cccgcagatc tgattctgca atcaatgctt
    79861 ctaaacgttt agtctgttgc gccttctcct cttctgtcca ttcaaaatat ccagcaattg
    79921 catcgatcac aggttgcttg atctcatcaa gctgatctcg ttcaaacaac agatgatttg
    79981 tacgacgaat gagataatcg cctggggcca aaaccatttc ttcttctaaa ccataacgta
    80041 accgagctga ctcagcgaga ctcaaaccag gataaggtgc catttctttt gctaattcaa
    80101 agatacgtcg ggcattagtc ccgtaaaaat cagcgatata agtagcatct tcttcctcta
    80161 aaccggctgc tactccttct tttgctaatt ctgtcactgt ttcttctaat ttagttggat
    80221 cgaagtttcc acctgaaatc tgatattttt tagagtcgat ttctttcgtc tctattccgt
    80281 attcttcttt taataattgg cgtatcaatc gcaaagctcc ttctgccatc ttacggtaat
    80341 cggtgatttt cccaccagat aaagtcagca aaccatctgg ttctctttct aaagaactac
    80401 ctctggaaat cgtagaaggt gctttttcat cacggctgtt ttccaaatgg ttcaacacgt
    80461 cttctacttc agcacgagaa actttgtttt ccttatattc acttactgta tcgacaactt
    80521 tattgaagct cttgtctgaa atcgatccat tatctccacc attgtaatca gaaccagaat
    80581 taccaatcaa cagtggacga agtcctgccc agcttgcttc gatatcattc aatgtgatat
    80641 ttgcttctgg atagcgatga ttgatcacat ccaatagata atctacatct tcttgtgtga
    80701 ctttcggatc cgtaaaatct ccttggtaat ccgtatctgt cgtaccaaag taagtcttgt
    80761 tttctcttgg aatagcaaaa accatccgcc catcttgctt tcctgtatcg aagtatgtgg
    80821 gttgcggtac aggcagtttt ttcgcatcta caactaaatg gatcccttta gttggacgca
    80881 ttttaggaga gactggacgc gtaaaattca agtttcgtac tttatctacc caaggaccac
    80941 tcgtattgat cactaatttt gctttgattt cgatcacttc atctgtcagc aggtcacgag
    81001 ctttgacgcc aacgatttga tcgccatcat ataaaaagcc aaccgctttc attttactta
    81061 ctagataagc cccatcttcg gcagcctttt tgatattatc aatcactaaa cgggcatcat
    81121 tgttgcggaa atctagatag acacccgcgc cttttagccc ttcttttttc aaaaacggtt
    81181 ctctttccaa tacttcttct ggtgtcagcg tataattctc atatttggtt cctgtcacat
    81241 ttgccagttt gtcataaagg tccattgcta ctttgaccga gaacatattg aaggttgttg
    81301 ctccttcgcc ctcatagatt ggcaaaagca ttggatctgg ttttgggatg tgtggggcaa
    81361 tactttgtac gactgcacgt tcaccaactg tgtcagctac tacttccaca tcaaatgtct
    81421 tcagataacg aataccgcca tgaacaagtt tggtcgagcg agaggaagtc ccttctgcaa
    81481 aatcctgcat ttcgatcaat cctgttttga ttcctgatgc tgctgactgg actgccacac
    81541 cggcaccagt gattccgcca ccgatgatca acagatccaa ttcttcttgc tgcatttttt
    81601 gaatgctatc ttgtcttgtc ttgtttgaaa acatatggat ttcctcctta ttcaaatgct
    81661 tgggtagctt ttactgcttt tttccaaccg ttgtaaagtt tttctcggcg ctcttcctcc
    81721 atttgaactt caaatagctt gccagcttca taaaattcgc ggatctcgtc tgtatctttc
    81781 caataaccga cagctaatcc tgctaaaaat gctgccccta gcgccgttgt ttccaagttt
    81841 tctgctcgtt ggatcggtac attcaagata tctgtttgga attgcatcag atattcattg
    81901 tttgctgcac cgccatctac tttcaataca ggaatcttga ttccagtgtc atcttgcatc
    81961 gtatcgatga tatctctcac ttgataagca atcgactgta aagtagcttt aatgatatct
    82021 tcttttgtcg taccacgtgt caagccaaac atagcccctc gtgctgcctg atcccaataa
    82081 ggagcaccta gcccaacgaa tgcaggaaca acataaacct catcttcgct tgtagagcgt
    82141 ttcgctgcgt cttctgaatc gctagctttt tgcaacattt gaagtccatc tctcagccat
    82201 tgaatcgaag aaccagccac aaaaatactt ccttctaaag catagtaaac ctttccattt
    82261 atgccgtaac caattgtcgt caataaatta tttttggata attgcggttt ttcaccggta
    82321 ttcatgacaa taaatgaacc tgtaccataa gtattcttga ccatgcctgg ttcgaatgcc
    82381 atttgtccga ataatgcggc ttgctggtct cctgccatcc ctgaaattgg tgcttcgctt
    82441 ccataaaaat gataaccttg cgtataacca taaacttctg agttagaagt gacttttggt
    82501 aacatcactc gaggaatatt tagtaaatct aaaatctcct gatcccaatc tagctgatgg
    82561 atattgaaca acatcgtccg actggcattc gagtaatcag taacatgtgc ttgtccatcg
    82621 gtcagtttcc atactaacca agaatcgatc gttccaaaca tcaattcgcc tttttcagct
    82681 cgttcttgcg cgccttctac atgatcaaga atccagcgta tcttcgttgc tgagaaatag
    82741 gcatcgatca ctaaccctgt tttttcgtgg aaaaattctt gatagccttc attttttaag
    82801 ttatctgcaa ttccagctga ctgtctggat tgccaaacaa ctgcatgata gattggacgt
    82861 cctgtatctt tttcccatat tactgttgtt tcacgctggt tcgttatgcc aatcccagca
    82921 atctgatttg gtttgatccc tgattcgatg aacgctccgg caataactga ctgtacagaa
    82981 ttccagattt catttgcatc atgttccacc caactttctt gcgggaaata ttgcgtgaac
    83041 tctttttgag aactgccgat gtgtcttcct ttcttgtcaa agataattgc tcgtgagcta
    83101 gttgtacctt ggtcaatcgc catgatatac gttgattctg tcattgtgat tccctccatt
    83161 atatgcaaac gctttcttta actactctta tcatactact gttttaaaaa aagaaactgt
    83221 caaaatttta ttcttttttt ctttttttga cagtttttta ttatgaagct attttttaat
    83281 caattctttt cttttttcag cgtctctatt cttcttttca atcgaataat gtcgtaaggt
    83341 gacgcaaatt ctgacagata atattcacac tgttttttgt ttctctggca aaatgtgatc
    83401 accaaatcat attctttacc ttcttcataa cttccaatct caataccgcc gattcccctc
    83461 agttctctga tcagatattg ttgaaaagca atacgataaa tcggcagact ttctaaatcg
    83521 attccaatca ctactggttt tgcaatataa aaatcgatca tcaaaagaac attggcatac
    83581 tggatcatca ggtaactaag ctcactatct tcagctcttt tcctcggcaa ttgctgcacc
    83641 gctgtttcct gtaatttcgc cagtaactgt gttaaggaat gcccgagcat tttctgctgc
    83701 tgttccaaaa gccgatcacg ctcatagact tcgatctttc caacaaagaa aaaccattca
    83761 ttgttgatct gggaaatttg gtagcctact gccttttctt ctttgatttt caaacgacga
    83821 ggacgataat gccataacat cgtttcccga atgtaagtat ctaaaacagc ggttggtaac
    83881 tttttgctcc gtgtcaaatc atactgataa aatatttctt cgtctactat cgaaaaagaa
    83941 acaaaaaagc tgtaaaacat cattgtttcg tatacattca gttctgctgc tgttcgacta
    84001 agatgcatca cgatgattgg atcgattgct tgataaagcc ggtccgactg atagagtaat
    84061 ttcttttctt tcaaagttgc gtacgtactt ttttcattca atagtctttt tttcgtaatc
    84121 ccgagccagc aagcaatctg tgcttctgct gatgccgtaa acgttgtttc taatacacgg
    84181 tccaatccta gaatgaacgg tcttttttct ggtgtatttt gcaaaaaaag cggtcgctga
    84241 tcttccggga tatattggaa tagttcataa taaaaatatc gtatctgtag ctcttctccg
    84301 tataattgac cgtttttgat ttgtaatcca aattcctcta agagctggtt caattctttg
    84361 atctttcgaa aaacagaaga ctcactgata ttaaacgctg tcgctaatcg aacgatggat
    84421 acttgctgat gttctaataa atagacgagc atcttaaact tcaagctttc ctgcaataaa
    84481 aacaatagaa ttttttccaa acttacatct tcgtccaaca caagcagcgc ctgatactca
    84541 tttcgctgta cttctacttt tttttccatc attcggccaa tcaattcaat atcttccaaa
    84601 tattgatcaa acgaggcttt tgacagttgg acatgctctc tcaaaccatt gatagaggct
    84661 gttccccctt gcagaacaag gtttcgcaaa atcaggatct gtaactgctc ttttctttct
    84721 aataatgctt caatcttcat aggcagtatt cgccaccttt gaccacacca tatctggcac
    84781 acggtattct ggctggaaaa atagctcatg ttcttctaac tgttcaaacg tccatccttc
    84841 ttctactgca attgtcagtt ggtcaagaag gtggaacatc tcttttttgc tttggagctg
    84901 tgctccaaga atcttttttt cagaatcaaa tagaaatttg agtttaactt tgctttgatt
    84961 tattgctgta agcttgtagt ctacgtatcc tgtttcaatt ttttggggat aaaaaatgct
    85021 ctcttcttct gtcaaaccta cgcttcctaa gaaatagcca aaccactgat tgccgactgg
    85081 acgataggtt ccctgatcct ttgtctgtga gccactaatt gtcttgctga catttcgtgc
    85141 tgtacggaca gcgttattta ctagagaaac atacattttt tcatttgttg gacgaaaagt
    85201 tacttggatc gcatcgccaa tcgcataaac gtctttttga gacgtttgca agtagtcatc
    85261 tacccagatc gttccatcat cattcaatgt taattgttct tcccacatct gattgtcggg
    85321 gcgagaatga ttagccagca aaacaagatc atagttctcc tttctttttt ccgtctctac
    85381 aatagcggtt tcattttcgg tcaaagacac aacttgttct tctaaaaaaa gtgaaagccc
    85441 ttgatttttc atttccttta ctaacggttc tgtcatttca ggatctagat aacgaaaaag
    85501 aattgacttt cggctttcgt aaagatggat ttgttttttt cttgatgcca acccttcagc
    85561 tatttcgatt cctgcttgtc ctgcaccaat aattgcaatt ttctttgcat tcatgatctt
    85621 ttgctgaaga gccgttacgt tttgtatctt tttaactggg tgaataagcg ttgattctgc
    85681 agaaatattt cgaaactttt gatttgaacc agtcgctaga atcaaacgat caaaatccaa
    85741 tttttttcct cctgctaaaa cgactttttt ttctttcaat cctattactg tcttttctgt
    85801 gtaaacatgg attcgatagc ggtcagtcag ttcttctttc gtgatccaag aaagttgttt
    85861 ttcactggta acctttcctt ttaatatcaa attgaacccg ctgggaatcc atcctaactt
    85921 cgattgcttt tcaatcaaat atatttcact tgttggcatc aattggcgaa gataaatagc
    85981 cgcatgaatc cctgcaaacg acccgccgac aattacaatg ttcatctgca ttcctctttc
    86041 ttataaggtg ttctactgct aacttctcaa tcagaaaaca cagtattcat aactcgcttt
    86101 caaccttatg atacatagcg atgcagcaga atcaaccctc tccatcatac cgtgttctag
    86161 gtctgcttct caataatgta ctagtggacc taattgaaga gtatcatttc tgaagagatg
    86221 tttcaaaaaa tgtactctta taaaagaaaa aaactcgcct tttacgtacc tctgtccaaa
    86281 taggaaacag aaaagaacga aaagacgagt tttagcgtgt gtaatgtaat agttagaata
    86341 gacacatcat ttatttttaa ttctttaatg cttcaacaac tgcttcgcct aattgatgag
    86401 cagattgagg attttgccct gtaagaagcc gcccatcaat ttgaaccact tctgaaaaag
    86461 ctgctccaga ctgatagttt gctccttttt ctttcaaagc atcttctgct aagaatggta
    86521 cttcattcgt tgtaccattt gcttcctctt cttcattact gaacccagtc actgtacgtc
    86581 cagctaaaat agattttcca gactcatctg taactggcaa caatcctaca acaccgtgac
    86641 aaacagctga gaccagccct tcatttcgat agatttgtga agcgactttc ccaagatcta
    86701 ttgcttctgg aaaatcccac attgttccgt ggccaccagc ataataaacc gctgcataat
    86761 catttgcatc cacatcatca ggacgtaagg tatttcccaa ggcccgattt ctgaaatctt
    86821 catcttcata ataggcccaa tcgatttcat ccatattcaa aacactttgc ggttctaaag
    86881 gtacatatcc gcctttagga ctgacaaaat ctgcatcgat cccatttttt actaatacat
    86941 cataaaaatg agtcagttca cttaaccaca aacctgtagg acgctcgatc ttttcgtact
    87001 tttctacatt tgttaatacg atcaatactt tcattttccc atcctctttc tataaaaatt
    87061 gcgttattga taacgtgctt tcaagaaatt atataatggg cttggcaaat aaagctcttt
    87121 atatgcttct tcaaaaggtt tcgttacaaa ttcatcctca cggttttcca ataatttctc
    87181 tgcccagtca ggatcgacga tcatttgctg tccaattgct gcaagttctg catatttcaa
    87241 tacctgctct acatcttcgc gattgcggat cccaccgaca ccaactaaag ggatctttcc
    87301 ttgaatcgtg ttatggacca cttcaagaat cgatttatcg ttgtatttat cagaacgagc
    87361 tattcgatca tacatattta atgaaacatg cagataatct agttttgact cacgtaatcg
    87421 ttcaagaagc cagatcgtgt cttcaaaacg gatacctggt gtttcatatt cttctggtga
    87481 gaaacgatac cctacgatga atggtttttt agcatattgt tcaaccgctg agaaaaccgt
    87541 tgttaataat tcttcaataa aacgataacg attttccaag ctaccgcccc attgatcctc
    87601 tcgtctattc gaatgcggag agaaaaattg ttggatcaaa tacgtatttg ctccatgcaa
    87661 ctcgattcca tcaaatcctg attcaatcgc tctacgtgtg gctttgccaa atgcttggat
    87721 tgtttcaacg atttcttctt cactcattgc tcgtggtgtt tccgcatcag gacgttttgc
    87781 tgcaactgca cttgcagata caggctgttc tccagataaa gttgctcgac ttgtcattcg
    87841 tccaccgtgg aaaatctgaa taatcgcttt tgcgccttct ccttggattg ctttcgctag
    87901 ctcacttaat cgaggaatca tttcatcatg agcgatgctg agttcacctg gccagccttt
    87961 tccgatatct tggacatttg cagcaccagt aataacagca ccaagacctt tagaacgttg
    88021 tttataataa gtgatctcat cttcggtgat cactccattg aaaaagctga gttgtgtcgt
    88081 cataggcgac agcatcaatc ggttcttcaa ttgaactcct gaaggtaaag taattgattg
    88141 atcaaattgt gacattgtca ttcctcccat aattcgctct gattaaccta tcgagaatca
    88201 tctatcatta aaccactcga ttagttgtac atgcaactaa tttgcttaaa aaattttatt
    88261 ttttttgtaa cttatctgtt gcatcattca ttgtctgggc taattcttta cttgcttcat
    88321 ttcccaaaac tgctttgtat gactgatgaa gttcttccca gctaccatcg acttccttag
    88381 caaattggcg tccttgttct gtaagataaa ctaatgacaa gcgaccttcg ctaactgttt
    88441 tgacgtatcc tttatattct aatttttcaa caaaacgagc aatcgttgat ggggcggaat
    88501 agagcatttg tgcaatatct ttttgtggta ttccatcttt ttcttttaat atcaataaaa
    88561 taaaaccata agttggtgcc agacctgtcc ttctaaacgc ttcatcagct aatttattca
    88621 acatgcgatc tagctttttc acgctaaaga aaagacattc atctaacgct ttttcaattg
    88681 gcttttccat ttcatcccct ccattggcgt acatatcata gtatgatttt tattttttgt
    88741 caaaaaacac ggaataatgt ccgcctcctc tgtctagatt tgaaaaaaag aagtagccat
    88801 cttcaaagaa tttcggcttg ttttcgacaa aactccttct ggaacaccgt gaatcagagg
    88861 ttgtgaaaaa gtcgtttagt tttgagtatg aagaaaaatt ttgtgaaaat ggctttccac
    88921 atttttgcat aaatttggct taataccgaa aaactggctt ttgaacaccg tttatatcag
    88981 aggttgtgaa aaagccgtcc tgcatcaagt aataagaaca gccaaacaaa aatagttcct
    89041 catattttgt cgttttttga gctaatgccg atggtcagct gattgtacac cgtttattga
    89101 gaggtcgtga gcctgccgtt gaactccgag tcacaaggag caatttcaaa aaacagcttc
    89161 tcatgtttta tgactatggt gacttgtgat cgaggagttg gcaggagaat actgtttatt
    89221 cgtaaagaag gggctgtgac aagacttttg tcacagtccc ttatttcctt ttattatagg
    89281 atttcgccat ttgattggat gacttgtctg taccaatcaa atgatttttt ctttaaccgt
    89341 ttctttgtgc cattcccttc gtcatcttga tcaacataga tatatccata acgttttgac
    89401 atttgtgaag tagaagcgct gacgatatca atgcagcccc acgacgtata accaaataaa
    89461 tctactccat ctttgatagc ttccttcatc tgctcaatat gctgtttcaa atagtcaatg
    89521 cggtaatcgt cttgaacggt tccatcttct tctactttgt ctatagctcc gagtccgttt
    89581 tcagctatga acagtggttt ttgataccta tcatataatt gattcaaagc aattctcaaa
    89641 cccttaggat caatctgcca gccccattca cttgatggaa gatacggatt tttaattcct
    89701 ccagttatta agtttccttg gaccccttcc caattttctg gatgagcact gactgcacta
    89761 gacatgtagt agctaaaaga aacaaaatca acagggtagg ctgctaagat ttcttcatca
    89821 cctacttcta tttttaagtt gacacctttt tcttcaaaca ttcgttgcgt atacgctgga
    89881 taatatcctc ttgcttgaac atcactaaag aacaaagaag ttctttgctg ctcaattgtt
    89941 ttttgaatat catccggatc acaagaatac ggaacaagaa gctgatagct gatcatacaa
    90001 ccgacttgtg aacctttgat aatttcatga caagcttttg tagctaaagc acttgctata
    90061 aactgatggt gtgcaccttg gtatttcgct tgttccaaat ggggatggtt ttcttcaatt
    90121 actcctacgc taacaaaagg gtgatgtttt acacagttga tctcattgaa agtcatccaa
    90181 tattttactt tatttttata gcgtttgaat actgtttcag cgaaacgaac gtaaaactga
    90241 ataagctttc gattaggcca tccaccatat tctgtaacca aatataacgg catttcataa
    90301 tgggaaagag aaacgactgg ctcaatccca tattttagac attcgtcaaa cacttgatca
    90361 taaaaaaata atcccgcttc attgggtgtt tcttcatcac catgaggaaa aattcttgtc
    90421 catgcgatag acatccgata gcatttaaaa cccatttcag caaataaacg gatgtcttct
    90481 ttaaagtgat gatagaaatc atttccgtgt cttttaggat aataatactt gctgttggga
    90541 tcctgcgccg cttttacttg ctgatgcgtc atcatatgcc attgatctgt ccatttttct
    90601 ttaggtaaat cgctgtcgaa tgtatacaca tcagacacag ataatccttt ttcatctaca
    90661 tcccatgcac cttcacattg attggccgct gtcgctccgc cccataaaaa tccttctgga
    90721 aattttttca tatcgatccc cctaaacttg ctagttttct tgtttgatta tttttcttat
    90781 gactagtaat gatatttttg aatctactac tatttgtaac aataaccatt gtggtcggat
    90841 catatccctc tttgattaat tcttgcaaat ctacttccat caataaatcg cctttacgta
    90901 ctttttttcc atcagatact gcactttgaa agtatttgcc atttaaattc actgtatcaa
    90961 taccgatatg gatcagtatc tctgctcctt cttcactgac taccccaatt gcatgtttcg
    91021 ttggaaaaac tgttgaaact tccccgttta caggactgta gatttttccg tccgttggaa
    91081 tgatcgcaaa cccttctcct aaagatccac tagcaaatgt tggatcgttt acttcagata
    91141 aatcaattat ttcacctgct gcaggagtac ttaacactat atcctttgag gattcttctt
    91201 cgatttcatc cttgtagccg aaaagaaaag ttagcataca ggaactgatc atcgctaaga
    91261 aataactagc aactagccaa acaaatcctt caccaatata aatcgggaaa gacaaaatac
    91321 ttcttgtagc aactgccaaa gcaccggctc ctgcggcacc agctactcca ccaaatatag
    91381 ctccacatat acaagcaatg taaaatggtc gtttatattt aagagtcaca ccataaacag
    91441 caggttcagt gattccaaat aatgctgtga taaatgcaga taaagataaa ctttttactt
    91501 tcttattttt cgttttcaaa aataccccaa gtactgctcc tgcttgcgca gctacggcag
    91561 tcatacatgc tggacctagc gtatcacgac cgtagaaaga aagattgttg gtcattactg
    91621 gtacaagtcc ccaatgtaac ccaaaaatca ctaacacttg ccaaaatccg ccgataacag
    91681 caccagataa aacagggcta aagttataaa cagctaaata tccagctgca atcagctgac
    91741 ttgcataatc tgcaattgga cctacaacta ataaagtaat tgggaccatc acgatgatac
    91801 aaatgaatgg tgtcaataaa tttcgaatac tttcgtgcaa gcttttgcga atcttaggct
    91861 caatataaga aagaacccat acagctaaaa ttgctggaat aaccgtactt gtataccttg
    91921 ctagaatcac gggcatccct agaaatcgca aggttatgct atcactatat gcactgacaa
    91981 tcgtaggata aacgagagct gctgcaataa ccatagcgat gaaacgatct gtattgaact
    92041 tttttgcagc agtatatgcc aaaaatatag gcaagaaata gaaaaatgca tcagctgctg
    92101 cattcaaaat gatataagtt cctgattcag tagttaataa attcaaactc gtacataagg
    92161 cgagtactcc tttgatcaaa ccacttcctg ccaagacgcc taaagtcggg gcaaaaacag
    92221 cggcgatcat gtctataaaa gagtttaaaa tgcctttttt ctcagttcct ctctttccac
    92281 tagatgcatc atcttctagt ttcatttgtt tggataatac gtcatacact tcatttactg
    92341 catttcctac tacgacttga aacattcctc cagcttcgac tacagtgatc acaccttcta
    92401 gttgctctac cttctcctta tctgccttct tattatcttt tagggtaaag cgtaaccttg
    92461 ttgcacaatg gtaaacattg ctgacatttt cttcgcctcc gacaaatcgg acaatatctt
    92521 tcgctaattg tgaatagtcc atttttactt cccccttgtt aattaatgcc gtagcaatcc
    92581 ttcttacaag ataagtatag ttcttagaaa acggtttctc cattcaatta gtacccctta
    92641 taatagcgaa cgaaactgta caatagtgtg tatggtttac ttagccgagc aaatcactca
    92701 ctgcttgaaa gaaaacaagt atatcgtttc tcttatagtc aaaggagaca aaaaaaacac
    92761 gcttgatctt cctttgctga tgtataagtg gtatctcaag ctgatactct gtgatttttg
    92821 tttctacttg gcttttgata agacagcaca acaaagtctc ggcatttaac ggaagcataa
    92881 ctaggtcaga actaggagag attagttgct gaatggaatc tatcttttta tggattttta
    92941 tttgattttc ttctttgtag taatgatcaa gcagttttgt tttctttttt tcttcaacta
    93001 tggtcagctt ttcacgcaaa atctgataaa tctcccaaga ttcaaactca tctagaacac
    93061 taggtagata aatcaaatca tcccactcaa aaataagctc ttgctcagta gtcaaaattg
    93121 catcatattc cgttattttg tttgattgat ttttgaaaag atacactggt tctatccggc
    93181 attctttgat ccacatgttc atactttgtt gaatctgttt ttttagtgta ttcaaaatac
    93241 taattggctg gttactaaca atcagtatct ccccttgttt tcgaaaagga ttcaacgctt
    93301 cggcaagata caaaacaaaa taagcgcttt cagctaaatt gggacgtaat tgaaaatgta
    93361 ttgggaagaa acgccgaatt aaataaattt ctaaaggata acgggagatg atcttttgta
    93421 caaaatgatt catggtatta tgtccggcat ccatcctcgt tttcatttgt ttgatatggg
    93481 ttagcaaaaa ttctgtttca tggaacaata acgaaacagg aagatctaaa aaacgaataa
    93541 tcgtttgctc aaatgctctt agattcttct ctatttcttt gtttttttca tcattaatca
    93601 tcaaataatt taactctaat agtcgatttt caattgcttg tttttcttga ttggaaaaag
    93661 aatagggtag ttctgatgat aacttttttg tgagggtctc taccatcgga ataaataatg
    93721 tatacctgtt tgttttttct aaaagttctt ctcttcgaag aatggtcaaa acgagatacc
    93781 gagaaaatcg tatgaagtcc tctcctgaaa caatataatt atatgatata agaacctctt
    93841 ttaatgtctt ttggacagtc ggcatgatat tggaaaaaat tgtactgatt tgttgaggca
    93901 accttgcgtg ttggatcacc gtttctgttc caacaagaga taagaaaatt ctttttgaat
    93961 tttcttcagc aatgattttt agtccttttg tcgacgaaac ttccagttct aatttaggat
    94021 tgcgctcgat ccaacgcaaa atcgttttta tttgtaatga tacggctgtt tttgaaagaa
    94081 agaaaatgtc tgctatcttt tgcatactga catacccttt ttgaaacaat aagtacgtca
    94141 caatagttga agctcggaaa tccatgctgg aatagatttt atgtttcgtc acctcttgta
    94201 tgatttgctg tttagtttca gattcaattg attctaaaaa ataacctgaa cgtttatttg
    94261 actggataat gattttcttc tcgtagatag caagcatatt tttgatttca gtttggatcg
    94321 ttttcttgtt ggtacctaaa gcttcagcta attcttcgct tgttatccaa tgattcttat
    94381 ttcttaatac tacaagaatt tggagttgtc gtttatttaa gtccatttgc ttacacctcc
    94441 tctttgttat aaacatacag gaaaaacctc tcaaatgaca atagtcactt gcagaggttt
    94501 aaaaatcatt tgaagaaaga tctatattca aggtttttac cattagactg gataatttgt
    94561 ttataatatt caaaactatc ttttttcaat ctcctaaagc ttccgctccc atcattttgt
    94621 ctatccacat aaataaagcc atatcgcttc ctcatttccc ctgttcctgc cgatacgata
    94681 tcgattggtc cccaccataa gtaaccaatg atttggcaac catcttttat tgcctcagca
    94741 atttcctgaa gatgtatttt tagatactca caacgttttg gatcgtggat tcgattcaat
    94801 tcatctggtg cttcatctag tccaatgcca ttttcaacta tgaataatgg taattcataa
    94861 cggtctgtca attcattcat gacataacga aaccctagag gatcaattgg ccaactccaa
    94921 ggttcgggag atttttcctt caaaaaggga ttatcgaaac catttgctcc gccagtatct
    94981 acgcgcattt ctgcttcttt tttatagaca gcgctacgat aataactaaa tgcaatataa
    95041 tctgccgtgt tagcttgcaa gcactgttcg tctccatcta acattactgg tgcacagtct
    95101 tcttccttcc atgtcctgta aatataagct ggataatgcc ctcggcacat gacatcggag
    95161 aaaaaccatg aattgcgttt aaattgcagt gaaccaaata cattctctgg atcacagtca
    95221 tatgcataag tcgctaatgc agaaaagcta cacataatac ccatcttagc atttggatcg
    95281 attttcttta ataattgagt agcacgggcg ttcgctagaa acatatgatg acaggcttga
    95341 taaatcactt tttttgttag tgtagcaatg ggttcaagtt tatctctagc agggaatgta
    95401 accaatccag cagctgcata aggcatacga aaagcattat tgatctcatt aaaggtcatc
    95461 caatatttca ctttcccttg gtagcgttca aagacggtct gcgcataaaa ttcaaaaaag
    95521 tcgattaatt ttcgattttg ccacccccca tactctccta ccaatgctaa gggggtttca
    95581 taatgactaa gagtaattac tggctcgata tcatatcgat ttaatgtatt gattagtctg
    95641 tcataaaata cgagaccagc ctcatttggc ttctgttcgt ctcctcttgg aaatattcgt
    95701 gcccatgaga tgcttgttcg aaaagctttt aaacctaact ccttcagtaa acgaatatcc
    95761 tcttcaaatc gatgataaaa atcaatcgct tcatgagaaa gataatggag tgattcgtct
    95821 aaagcaatct cccaaatctt tttttcttca ttccactgaa tactaggatg ctggttcatg
    95881 atcccgataa gcgtatctgt cacattaggt aatttgccgt cttccaacca tgcaccttca
    95941 cattgattag cagctactgc tccgccccat aaaaaatttt ctggaaatat ttcctgatcc
    96001 ataactatct ctcctcgcta tcaacaattt cttacttgaa gtatagctcg attcatcaaa
    96061 acaagatatt ctaactattt cggcagaaga gaagaaaaaa attgaatgct actcttcatt
    96121 tttaatattt atatataaca aatacataag acgatccccg aaagaaaaca ctcgttaacc
    96181 atctaagcaa acgatttatt ttacttccaa cacaaatgct tcataaggtc gtaaatcaac
    96241 tttgccgtct ggtaactgtt tcagaggata atttgacaca ataaccctta aagcttcacg
    96301 tggaacagtg aacgtctgct tttcactgga catattacaa ataacgagcc attgctggtc
    96361 ttcatatatg cgagaatagg caaagacatg ctcaggcgtc ttttccacta attcatagtc
    96421 tccccagacg atgatgtcat gttttctacg caaatcgatc aatttcttgt acatataaaa
    96481 aacagaatct tgatctgcta acgcttcttc tgcattgatt tctttataat ttggattgac
    96541 tcgaagccat ggaattccag tagtaaaccc agcatgttca cgggcgttcc attgcatagg
    96601 cgtcctagca ttgtctcttc ctttcgcatt gattgattcc aagatttcct cttttgtaaa
    96661 tccagaagaa atcctttcgt tatacatatt gattgtctca atatcttttg cttccgcgat
    96721 atcatcgatt gggcaattcg tcatgcctaa ctcttctcct tgatagatat aaggggtgcc
    96781 tttcatcata tggagcaaga tagcaaacat cttggcactt tcttttcgat acacatcgtc
    96841 atttccccaa cgagaaacaa ttcgcggaag atcgtgatta ttccaaaata aactattcca
    96901 tccttcatca cctaaagatg tttgccattt ggataatact tttttcagtt cagagatact
    96961 caagggtttc aaatcccatt tgtctttgcc ttcttgctga tctaatccta tgtgttcgaa
    97021 ttggaagacc atggataatt catttctttt aggattggaa taaagtttag cgatttcggt
    97081 agttgctccc catgtttcgc caacagtcat cagatttttg tcaccaaaag ttgcttgatt
    97141 catttcctga agatactcat gcagtttagg tccattactt gtgatttctt tatccggttc
    97201 tttgccaatc atatcgatca cgtccatacg aaagccccca atacctttat cgatccagaa
    97261 attcatcatt tgataaatct cttggcgaac ctgtttattt ttccaattca aatcaggttg
    97321 acgcttgcta tacaagtgca gaaaatattg ccccgttttt tcatccaact gccaagcaga
    97381 accactgaat attgagcgca atctattagg ctcttttcca ttgaccggat cacgccaaat
    97441 ataatagtcc cgataaggat tttctttatt tttacgggcc tcaatgaacc acggatgttc
    97501 atcagaagta tgattcacca ccagatccat gatgatacgg atatttcttt tctttgcttc
    97561 ttcaatcagt tcttccattt cttccatcgt accgaattct gccatgattg cttgataatt
    97621 actgatatca tacccattgt catcattcgg agactcgtat actggagaaa gccaaattgc
    97681 atcaatacct aaatacgcca gataatccaa cctggaaata atcccccgca aatctccaat
    97741 accatcttga tttgaatctt gaaaacttct tggatagatt tgataaacaa ctgaatggtg
    97801 ccaccatttt tgttccatga ttttcctcct ataaatggat gtttctattt tttgcttaat
    97861 catttatttt agtctctctc actaaaaatt gtaacgcttt caaatagcta ttgcaccttg
    97921 ttatcggtaa cagaataaaa caaaaaaaca gctgaggaaa ttccctcaac tgttttttga
    97981 aaggattgat ttatgcaggg aagaatgctg atttcacaac ttctttataa acggtgttca
    98041 ctcagctgtc ccttggtatt agctcaaaaa acggcaaaat atgaggaact attttttcgt
    98101 ttttttctct aatactcagg ccagaacact gatttcacaa cctctttata aacggtgttc
    98161 actcagctgt ccctcggtat tagctcaaaa aacggcaaaa tataagaaac tattttttcg
    98221 tttttttctc taatactcag gccagaacgc tgatttcaca acctctttat ttcacattca
    98281 atatccacat gaatcccaca aatacaaaga acagaccata catgattgga gatacttctt
    98341 ttcctctttt agctgctaac atggtaattg gatagaagat aaaacctaat gcaatcccat
    98401 ctgaaatact ataagtcaaa ggcatgccga tcaagatcaa gaatgaaggg atcgcaatct
    98461 ctaagtttcc ccattcgatt tgtcgcaaag attgtgccat caatactcct accataatca
    98521 atgctggtgc agttacttgt gaagtaacaa ctgatagtaa tggagaaaag aacaagccta
    98581 acaagaagaa aatccctgta gtaatagctg tgatacctga acgtcctcca acggcgatac
    98641 cagctgatga ttctacaaat gcaccaacag gagaagtacc aaataaggaa cccgcaagca
    98701 tagctgttga atcagaagct aaagctttcc ctacacgagg catcttgttg tctttcatga
    98761 aacctgcttg gtttgctaat ccaactaaag ttcctgctgt atcaaagaaa gtcacaagca
    98821 agaatgtcag cactacgacc cacatttgaa gagaattgat gtcccctaca tgttttaatg
    98881 caactaaaaa tgttggtttc aaacttggtg ctgcggcaac aatttcagaa ggcattggaa
    98941 tcaaacctgt gaccagacct aaaatcgttg tagctgctat cccgatgaag atgccacctg
    99001 gtacacgccg aacaagcaag atagcagtta cgactaatcc aaaaatcgtc aaccaagtag
    99061 aaccaacgtt gagtgatcct aatcctacta atgtagattc attcgaaacg atgatcccac
    99121 cttcgctcag accaaggaaa gcgataaata gaccgatacc gccagaaatc gcatatttca
    99181 aatctgctgg gatcgcatca atgatcattt cccgcagctt gaagattgtg atcaaaataa
    99241 aaatcaaaga agcaacgaat acgccggcta atgctgtttc ccatggaatc cccattccta
    99301 aacaaacaga ataagcaaaa aaagcattga tcccaagagc aggtgccgtt ccaattggat
    99361 aacgggctaa tacccccatt aaaacacagc cgatcgcact ggctaatgca gtcgcagtaa
    99421 acactgctcc ttcatccatt ccagaagcac ctaatacagt tggatttaca aataagatat
    99481 aagccattga gataaatgtt gtaaatcctg ccagtacctc acgttttacc gtggtgttca
    99541 atttttctat ttcgaagtag gaaagtattt tttccttcat cttccatccc cctaaatccg
    99601 aacattataa ataaagtccg ttaaaatttc gatttattaa taaaacaacg tgttttatca
    99661 tatactaaaa caaaacataa gtaaacaaaa aattcggatt ttaagcaaaa tccgaacgtt
    99721 ccatctgatt aaatcccttt atctcaaagt tcatgaacat tcactttgga ttacaagaat
    99781 ttctttattg tgttaataaa gtttcttcat tctagtctca aaaatcaata gtgtgatttg
    99841 attttaccat ttcgtttctg ttcttctttg atgaattctt ttgtatcgac aggattcaga
    99901 ttatttagtc cgccaaaacc tgtatcgacc atttttcccc agccttttgt cttctttttt
    99961 cctgatttga gattctttgc tcgaagcttc tcactatgtt ttgctcgccg atacttcaaa
   100021 taaccttcta atcttcccat tagatccact gtttttcctc ctttttattc aatcagacga
   100081 ctcagctttt cctataacaa aataattgcc ttcagggtca gaaaaattga aagtcaagat
   100141 gccattgtta tctgtgattt ctcctggttc ttccatgttc tcgtagatag tttcaaattc
   100201 atccgtaaaa aatactaaag aaggcggtgg tcctaatacc tctggtgaaa acttctgcac
   100261 aaatactttt gggaatacgc ctaattctaa ttcttttgag atgctgagta caatactctt
   100321 aaattcttct ggcaattcga ttgtttcaga aatttctgca ccaatttttt ctacaaagaa
   100381 tcggcaaatc atctctacgt cattgacata aagcatcaca cgtgtttttt gtatcatcat
   100441 ttgacccttc tttctatttg attttctttc ctgtctataa actgattcca ttatatcacg
   100501 aaagcgcaaa caccttgtgt ttgatactat tcaagaaaaa taaagcaata gtttctagga
   100561 ttgctttcct ccacaaactt tttttacaaa aaaaaaaaac ggcaatgaat gccgctcttc
   100621 ttatttcgat tcttttataa ttctgcttct gattcatctt tggttagtgt gtgtctaaca
   100681 ctagttacgc caaatgtttg atcgattttt tccttcaacg cttcgattgc ctgtgcgcca
   100741 cgtgccgctt gttcagcatt gatggattca atgaccagca ccgctgcttg tgtttcttcc
   100801 gtcagcattg ttctttgggc ttcacgccag ttgagacagc gacagatcgc tccttgttca
   100861 tcatagtaga tgatttcttc cggaagtgcc ggagcatcgg aatctgcacc taaaggcagg
   100921 aatgattcgc caccttgggc ttttcctaaa tgcagatttc ctgcgatctt ttcgatatct
   100981 tctccgccgc aaggtacgcc aaaagacaag gaaatactat tatagatatc tacaagcgga
   101041 ttgattggga aaaattcccg ttcttgattt gcacgtttca gtaaagcttc aatagaagaa
   101101 cgtgcacctt ttttagtctt gaatgtggta aatgcctgac gccactgagc aatgacttcg
   101161 ttttggctga atgtttcttc tgtaaggtaa tttcttgctt cttttgctgc ctcattcaat
   101221 aattgatgat acgtttcttc actttctttt gttacgtggt tatcgatacc atttacggat
   101281 aagatttcga tggttgcttc aggaaatact tcccagaaat tggtatccac tattactttt
   101341 ttcatctgtt cacacctctt gattagaata attgcttgat gaatagatag tctgtttggg
   101401 gatcatctcc catataaaaa acatgttcgc tgtatttgat aaatcctttt ttacgataaa
   101461 aagcgagcgc aacatcgttg tgttcccaaa caccgagcca aatggctgtt ttttgctttt
   101521 cttttgcttt ttcaatagct gtatctagta aaaatgtacc gataccttga tgtttaaatt
   101581 tttttcgtac atagattcgc tcgatttcta aaaaatgatc agccatttcc tcagattgac
   101641 tcgttcctgt atttagtttc aggtaacccg caggctcttg atcgatactc actaggaaaa
   101701 aatcggtttc tttatctttt agctcttctg taagtttttc tagtgcataa gcttgtgtca
   101761 agtaatcagc taaatgctcc ggtgaattat cttttccaaa tgtatctgta aatgtttcga
   101821 tactaatttg ttgtagtaag tctacatctg aaggcgtcat ttttcgaatt tcgtatttat
   101881 tcacggcttc ttcctttcaa tagatccttt tattgccttt tttgacaaaa ttccaatctt
   101941 cgctaacatt cttgcatact ttatttaaaa gatattccaa ttgttttgct tcttcgtcag
   102001 aaagaccatt taatgcgact gcattggagt agttgttttc tcgaatgatg aaaggataga
   102061 tttctgctcc ttttttagtc ggatagagtt tcttgatttt tttattttct ttatcttcca
   102121 aacgttcgat catcccattg gattctaatt tcttgattgc tcgggcagtc gttgtacgat
   102181 ctactttgat catttctgct agtttttctt ggatgattcc aggattttcg caaatacgta
   102241 ctaaataaag atactgtcct cttgttaaat cgacttcttt gaattcgata ttgctaatag
   102301 aatctagcgc tcgggcaatc attccgatgt ctcgaaggat ttcagccatt tcatcccacc
   102361 tttattttgt ttgtaagact agtttacctc tttttgttgc aaatgcaact attttcaata
   102421 aaaaaatcaa gttctttttt aataactagt aaagtatatt cttttcttct tttacgaaaa
   102481 agctcaatca caatagatct ggtttttgtt ttctaaaata agtaactgtt tcttcgttgt
   102541 tcgcttttct gatagctttg gcaactcttc tatactaaaa aatcccatgt tggaagtttc
   102601 attattctca ataaacttgg cctcttcacc gtggatcgca caagcaaaaa caagtttata
   102661 atactgaaaa agctgtggaa tatctttttg tttattcgta tcaaaaactg ctcgcaactg
   102721 agtagtttct actgttaatc ctgtttcttc atagacttct ttacgcacat tttcttcagg
   102781 agacaatcct atttcagcaa atccaccagg aagtgaccat tctttcgtcc gtttatcttc
   102841 gattagtagg accttgccat cttttttgat ataggcacga acatctactt ttggtgttgg
   102901 atgtccttct ccaatcgttg tgagcgcttt tatttcttcg aaagtttctt ttcctatttc
   102961 actgatcagt tccaatgaaa tcgtccggat ctcctcaaaa cgctctttat caaatacatc
   103021 ttttccataa tatagaccag cttgagaaag tgcgagtagt cttttgtatt gagccaactt
   103081 cttgttcatt ctcttccctt cttttttagc aaatgtatga ctatgtaatc aagtatagaa
   103141 gactggttta attacggcaa ataaatataa tgaaatctgc aataaataaa agaatgttaa
   103201 aaaactcgag aacaaaagac attcgacttc tctggggctg tgaataaatg gcgttcaaaa
   103261 gcttgcatct gcggtaataa gccccaccaa acgaaaaata tgacgagcta ttcttgtctg
   103321 gctgttctta ttaattgagg caggacagct ttttcacaac ctccccacta aacggtgttc
   103381 tcctaccaac tcctcggtca caagtcacaa tattcatcaa acatgagaag ctgttttttt
   103441 gaaattgctc cttgtgactc ggagctgaac gacaggctca cgacctctcg gtaaacagcg
   103501 ttcaaaagcc aatttttcga tattaagcca tttctttgtt acttattgtc attgatttct
   103561 ttttctaatt tatccaatag tggagtgtaa tccacttgag tatagtctgt tacatcaaat
   103621 tcatatagtg ttcctaactg gaatttgtca tattttcggt cggcgatgat ctttttgaat
   103681 gcttctttcg taatatgatt ggtccctttc gttcgatctt ccaatacgtc accatagtga
   103741 taatgatcca tgatatagct caaatgacga tctgattcac gatcacgttt gtaccgacgt
   103801 tgacataaca cttcaaagtc tgctgttagg cgaatcgtga tcacctgata tccgtatcta
   103861 tctgctaatt cttggagtcg tgctttttgc ttatcactaa aaggatactc ggacacaatc
   103921 actttctttc ctgcttccat atacaaatca agtgctttgt aatagaaagg ataaactttc
   103981 gtcctttctt gttccgcccg ttcttccaaa ttgttaaacc cgatggattc tgcatagtat
   104041 tccttgattt catcgagcgt gatggcatac atatctggaa atcgttcccg tagtttattc
   104101 ataagatatg tttttcctgt accaggacta ccagccagaa gaatgaaata ctttttcatc
   104161 tcatttattt cctaagttaa ttggactttc caagttcata aacagtccaa tcttatgctt
   104221 gacaatttct tttactgctt catttgctgg aatcacatta tggcgaagag acttgttgat
   104281 ttctgtttca ctaaaggttt ctttagctac tttcgtccag ttgactaaaa gctccgttcc
   104341 aacattgaat ttgcggatcc cgttattgat caattcagga taatcttctt ctttcacacc
   104401 tgttcctcca tggataacta gggggacatc aactactgca tcgatttctt tcagcaaggg
   104461 aatgtttact tctgtttttg atttgaattg tccatgattc gtaccgatag caatagctaa
   104521 tgcatccacg cctgtagctt ctacaaattt cactgcgtct tccggtcttg tatatacctt
   104581 gtcattttca gcgacatgga ttccttcttc tgtaccgccg attgttccaa gttctccttc
   104641 tacggaaaca ccgtgcgcat gggcatattc tactactgct tttgtcttca ggatattttc
   104701 tttgaaaggc agatgggacc catcatacat gacagaagta tatccgctgt cgattgcttc
   104761 tttgatatca tcaaagtctc gggcgtggtc taagtgcaag actgcatcaa ccatttcttc
   104821 ttctgcaata gctgtcatta cattgactaa cacatgcatg ccgatatatt tagcggtatc
   104881 gacacttgtt tgaataatga tcggcgaacc catttctttt gctgcgccta gcatagcagg
   104941 aagcatttct aggttatgcg tattaaaagc tccaactgta aaattcaact cttctgctgt
   105001 ttttgttact tctttcaatg ttgtatacat cttttattgc caccttatct ttttatttgt
   105061 ttcctttgat cgttacttgt tttgcaattt gtcgcatctc atccattttg cccatgtagt
   105121 aatcgatccc ttgatcatca ccagcacgag cagcatctgc acgcttctgg ttatagaggc
   105181 tcatcagttt tttataccca tctccgatcg tctgtttatt ttccaaactc ttttctaaat
   105241 attggatggc ttgatcgttc acatacaatt cagaaaattt aagtccaact tgttcataaa
   105301 gtttcgctaa tttatgctta tcttgggtag tggaaatctc ttgtttcaat tgtgtaatct
   105361 ctgattgaat cttttcggtc gcttctggcg atagggcttt ttcttctttt gccagaactt
   105421 ttttcggttc tttttctttt ttcttaaaaa atccaaacat cattttccct actttcaatt
   105481 aaaatgcttt caaaactggg cttaagatcc aggcatataa gagtgctgcg gctactgtat
   105541 ctacatcggt tgctgtagca ttgatgaatc ccatcgaatt aaagatcgtt actagcaaag
   105601 cgggcaataa tgtaataaag aaaccatgag caataccgcc aatgatcgct cctcggcgtc
   105661 cgccaactgc gttgccaaat atccctgctg tccctccggc aaagaaattg gttagcatac
   105721 caggtaaaat catcgctaag ccgaacatgg gcaaagtaaa catggcaata atcgtaccaa
   105781 tcgttgtcgt aatgaaacct aaaatgacag catttgggct ataaggaaag aaaactggac
   105841 aatctaaggc agggatcgca tcaggaacta atttcatagc tatccctcga aatgccggca
   105901 cgatttcacc taacagtaga cgtacaccag ctagcaatac atacacaccc actacgaatt
   105961 ggatggcttg taaaaaggca aacatgatat agttttgatc tcctgataat tcagaagcga
   106021 atggttcgcc tgcaaataaa acagtgatga tatatagcgg gaccatcacg accatgacag
   106081 acaaataagt atcttgcaga aattcgaaat gtgtcggcaa cttgatatct tcgatcgact
   106141 ttttattttc gcctttctca ccaaaaagct tagcaacacc ggcttcaaat aagtacccga
   106201 tcgtacaaaa atgacctaaa gcaatatcat ttgatccagt gattttacga atgatcggtt
   106261 gcgctactgc cggcatagca atggcgaaac aagctccgat aaaaccaccg accaagatca
   106321 atacgatccc tcgcagaccg gcaaaatagc caaagactgt cgtcatcgtt gccatccata
   106381 aaattgcttg accagttaaa aaaatgtact tccattttgt gaaacgagcc aaaataatat
   106441 tgaatacaaa aatggctaag aacgtcagtg caatatctct acctaaaccc aaatcattca
   106501 ttgcttgacc attgatagat tcaatagaag ggatgatccc ttgcatatga aatccttcag
   106561 taaagatttt cccgaaataa gttaaacttc caacgatgat gctcgaaccg gcactcaaca
   106621 cttgaaatcc taggagtgtc tttaacgtgc cagagatgac ttgtcctgct gatttcttct
   106681 gcagtagtag acccaacata gcaatcaatg caatggtgat cgatgcctga gtcaaaatat
   106741 tttcgatgat aaaattgata acgcccatgt ttttaccctc ttttcttttt gtatttagat
   106801 aactccctta tcttgcagaa ctggggtgat tttttcttca atttcatttt ttgaaacaat
   106861 atttttcaaa tagatcatcg ccgttttagg atcaactgcg aacttttcga actgcgaacg
   106921 aaaattttct gctgtgatga tcacatctgg tttcatggaa actgcggaag aaatatcaca
   106981 atggtccagt ttggcttcta ctcctaattt gttcaaaaca tcctcagcag acatctgtgc
   107041 tgcaaaacta cttcctaaac ctgcaccgca tacgaataaa atgttcaatt tgttcatctt
   107101 tgttctcccc cgtatcattt attatttctc aactactgaa aaaagcagtt gtttgaaatc
   107161 ttcgatattt gtgcattcaa tcaatcgatc caacttctct tcgttgttga tcaattcaat
   107221 tagttctttc atgatgttca gatgcgagta ggagtctact gctgccaaac aaaaaataat
   107281 ctttactgga tccatttcag gattaccgaa atcgattggt tgctcaatcg tagctacgct
   107341 gactcctaat ttattgaccc catcttctgg tcgggcatgg gctagtgcca tgtgtttgcc
   107401 aatcacgata tacggtccgt aatcattcac tgcacgaatc atcgcttcga catagcgctc
   107461 ttcgacaatg ttttctttaa ccaatggtct agcagctagt tcaatcgatt cctcccaagt
   107521 agtggctttt tctttgatca tgatattgct gtccgtcaaa atatctttta acataggctg
   107581 gatctccctt ttgttgattt ctaaattgtt tgtttcgaat aatttttcta acttgccata
   107641 gatgattcga tctactcggc caccactttc tttcatgaca tctattattt gagtgaataa
   107701 ctccgtcgat tcatgactgt taggctctaa ccgctgtagt tcttcatttc ttttcaaaaa
   107761 gtcttctata accaagatat tcgtctcagt taaaatcgga tcgacaacta aaagcggctt
   107821 atttcgataa ttcaattgaa aggtgctgaa aaccagattg acatccaatt tatctactaa
   107881 ttcgatttct tttgaactta aaactgcagt aatctcgatc tgaggaaact tttctttcag
   107941 attctctgct aacagatttc cagtagccat cccatggtta cagacaacga ctgatttata
   108001 aacataattc aagtctcgct tgattacact ggcgatcgtc gaaaagtgga tgacaagaaa
   108061 agctatctcg tcctccagta tcttgccacc aattacttct tcgatcgtag gaacaaaatg
   108121 agtgactgcg cggtagattg ggaaataatt tgttcggatg ttttctttca atggattcat
   108181 gacttggata tgattcttga ttcttacaat cagtgcagcc atgtgcttat agagattttc
   108241 gaataaagct tcttccttta gatggaaagg tacgtgtgtc tcgttctcta caaattggat
   108301 caattggatc gttagtaatt gagcttgtac ccactcgatt gaattggaga tatctctagc
   108361 attgaacgtc tcaatagtga aacgaagata atttctttct acttgcggga cattgtccaa
   108421 ttgaaattcc tcgatcatgt ggtcaataaa tgcagaaaag ctactgtctt ttatttctgt
   108481 atttttccaa ttaatggcag agataaattc atgtttcctg attcgctgga tccagatcaa
   108541 tgtaaaaagc aggatttgtt ttttataaat atcatcttct tgtttcaaca acttattgga
   108601 atagatctcc tgtatttttt tgatttccac caaaggaaaa taatggagaa agattttttg
   108661 agccggtgtt aattgtgcat cttgattcat aaagaagtcg gtcattccca gattctgatt
   108721 gataaaatca tagatcatcg tacggataga tcgttctgaa cctttataaa ccagtccttg
   108781 ctttccataa ctgacgatct ctatcccata actgaccagt gttgctcgta accgtctcat
   108841 atcttcatct aatgtacttt tagagatttg aaattcttct tcttttttgt ttagatggat
   108901 cgggattcgg ctgtaagcaa cggctaaaac tagatcaaaa atacgttcgc gccgattcaa
   108961 aaagccgtct ttcacatctt ttatttcagt ttctaatcga gcttgctttt ccttttccag
   109021 cttcaacaaa aaaactttgt tgcgaattgt acggatctcc ggaagctttt tactgctcaa
   109081 ccagatattc gcttcaagaa cttcctgacg gatcgtccgc tgactgacat tgaactcact
   109141 tgctagatgt tttgctttta ctggatattg ggctgtcatg attctgtgaa gtatttcaat
   109201 tgttcttgtt ttcatagaaa tgatctcctt ctgatttcaa gtatatggtt ccgcttacat
   109261 aataaaaagt agatatttct gccacttgaa gtggaaagaa agtaagcaaa gcaaacaaaa
   109321 aataagccat atgctttttc atctcctcaa cgaaaaagac atacgcctat tattacaaaa
   109381 attttgccca tctatacaaa aaagaggttg tgaaaaagct gtcctgcatc aagtaataag
   109441 aacaactaaa ctaaaatagt gcttcctatt tttcgtttgg ttgttcttat tatcgcagat
   109501 gcaagctttt aaacaccatt tagtggagag gttgtgacag agatgttcag ccttgagcga
   109561 taagaagcga ttttgaaaaa tcgcttccca tattttacaa aatcgtgcct tattgccgat
   109621 aggctatctc tggcacactg tttattcaga aaaaggagct gcgacaaggc ttttgtcaca
   109681 actccgttca taaatatttc ctgtcaatcc agcataatcg gctgaataaa atcggcttgc
   109741 catctttttt ctttctcaga aaaatcaaaa taccatatct gagaaaaata ctgtcgttgt
   109801 gtcttacttc ctgagatcaa tcgcccatct tttcgatcga ctgaataatc acagcatctg
   109861 aaatcaattc ggatggcaaa actattttcg tggatctgtc gataatttcc tacttcctga
   109921 atagttattt ttttagtatg atttcttaca ccgtctgctt tgtttttttg cagaactttc
   109981 tgatgttcgg caaacagctt gtctgtataa cagcttctgg ctttttcagg agtttcttct
   110041 tcccaagcgg attggatctt catgaatatc ttttggattt ccagaaactg tgctttcttt
   110101 tctttagccg ttcctgacat gcgggacaat atctccgctt tcagcctttt cttttgtctt
   110161 ttattcttga cgatagaaaa agcagaaacc cctcctactg acaaaacagc taatgcaaga
   110221 aatcccgttt gctcataact agaaggacct gtacctggac cataataccc ataagaatct
   110281 ctgccgtaag aacgactgcc aaaataactg cccccatatg aacgaccacc aaatgaacct
   110341 ctgcccgaac ttcgaccgct agatgtacct ccaccatgac cacccgcgac agcgaatcct
   110401 tgggtcggca gagcaaacaa acataggccc actaacaaaa tgattgctaa ataattgatt
   110461 tttttcattg tcttcctcgt tatcttttat tacaaaaaac tatttttatg atagccttgc
   110521 taaaaggaag agtcaataac cagatgcata aactgttttt ttagcaattt tgtcttattt
   110581 agtcattgag taacatcgga caaatgtatg tgtcggcagt ccataattcc tctatcttca
   110641 gcatgaacaa gcttgcagac aagagatctg cgcttcctcc aggagagatt tggttttcct
   110701 gacaaaagtg caacagtgct gtaaatggac gatttggtaa agcactttga ctagcagcat
   110761 gagcaagttt ttgcatttcc actaacgttt tctgatcact gcgatgcaat acgcatgtgt
   110821 catctagcga actcatcaaa actaacaaca tatgcatttg tttctgtaca tatgaatcat
   110881 tatctagctc cttagctgct tggattgcaa gcctgatatg cggataccct aatatcgctt
   110941 cttcatatgc accatttacg ccatattttt gcttcaccct atgaccatga gtggtttttt
   111001 tatgacgata ttttgtatca ggaaaagcag caagcttgct gacatcatca agtattttcg
   111061 ataaagaaaa aggctgttcc atactgattt tttgtgcaat gacactgatc aatagcccca
   111121 ttgcccaaat cgctccttta tgggtattga tgccatttgt agcaatcagc attgcctttt
   111181 ccgcttttcg cccaatctca gcaattcttt ctcttaactc ctgatcaata gcatgatgcc
   111241 atgctatttg agccatttct tcaaaaaaag gagttagcgt cttagcagac ttaagaaata
   111301 aagcatagtc catatcggaa tgtgcacccg catccagtgc atccactaat ccaggttttg
   111361 gacttaatgt tacttctgcg atcaatgctt cctctaccaa cgcagcgagt tgctgagtca
   111421 ctgctttttt tcgttcgtca tttatcatga cactcactcc ttcttcctac tattacttgc
   111481 tgaaaccttt tttgatagct acccaaatga tatgaagtaa ccagcttaaa gcaaatgcac
   111541 aacttgccgt cattagataa gctacaagaa tcatgactcc tgaaggtaac tgcagccaac
   111601 tatctttaaa caataataac agtacttgga aaacaaatac attggctaaa tacgtacgat
   111661 aagcataaac agacaaccat ttgaacaatg gcaattttgg cgaatcgttt acaatcattt
   111721 tggaagcgcc tatgaagaca gcaaagatga tgactaaact gtataaactt tggatcgtat
   111781 tcaagtaagg tgcattcgca aagctcaagt ctcctggatg tgcgagcaat gaacggacag
   111841 aaaagaacgc gataactaag gcaacaggta agaaaagata acgtactctg tccaaatatt
   111901 gatagacttt ttcgtgataa acaaatgcgg ctaccccata aatcatgaaa ctagcaacaa
   111961 accgatctaa taagtaccag ctgtcatgat aaggcccagt caatacttgt gtttgataga
   112021 acagatacca tgcgacatat aaaatcgttg aaccaatcaa tactggccat acatattttt
   112081 tattagcaaa tactttgtaa ccgagccaaa caaaaaacgg catgaacaat tggatctgca
   112141 gcatcataac ggtataccat aaatgaggcg ctccatcacc tgtgacatat ttcagtaaaa
   112201 agcttgcagt atccgtatac ggagtttgtt gttggactgc tggaaatagc caaagataag
   112261 ccgtcgtcca tagaagatac ggaacgacta gttcaaaaaa cttttctttc aaaaattccc
   112321 aataagacaa atgctgactt gtttttacca tattataaac aattgcaaaa ataaacattg
   112381 gtgctgtgta tttaacgatg acgtaaaagg taccaagaaa agctgtattt gttgcgctgt
   112441 ggctgtttgc caaagcaaag ctcaatatcg tttgcatcat aacagccgtt acagctgata
   112501 ctttctggaa gtcacttacg ttgattttga tttttttgtt ttcaaacatc tctttctctc
   112561 tctttttctt tattaccaaa gttctttggt taatttaggt ccttcgactg ttttcaatag
   112621 aatttttttc tctggctctc tcaaatactc ttttagtgaa aaaccgcctt gccgagtcac
   112681 tacttgggta tcgaattgct caaaaaaagc aggatgcgat tgaattacct gaattggcaa
   112741 ttcaatcggt gaatttgcgt acagcagcaa atcaaaatca ctggtttctt ttactgccgg
   112801 aatcccagtc gataattcaa atcctaagct gccgccgatt ccccatttac atcctgataa
   112861 tatgtttcta gcagcatgaa attgctgata aacaggataa tccatgattt tctcttgtct
   112921 atacgattct tgttcgatga tgtcccacgg atgaatgact tgtttgacgg catgttttga
   112981 gatttctgtt gaccatctct gattgcgggc aggtccacgt aaaccaatcg gtagtatttc
   113041 tgcgtgatcc gtcgacgcac gacgaactgt tccgaacatc ttttcagtta gaacgaccca
   113101 ttcaggcaag gtgaaccctt ctatttcatg tttatcaaaa aggacaatat catgtgcttg
   113161 aatcattttt tatgcatccc attcttcgtc cattttggca cggactttat ttgtttcgac
   113221 acgtccgaat gttttggctg tttcgtttgt gtatcgattt gtcagcatcg tgtcatttgt
   113281 attgcggaca tcttgaatgg cttcattgat tgcttcgatg actttgctga cattttgttc
   113341 tgaagcagct tggtcggtga tatcttctag gaaatgatag atcgctccta gttttgtata
   113401 attattgata tcgtaagcga tagctggaac tttttcagca gctgcttcga tttcagcaac
   113461 cgaacgtttt gtcacacgtg cagcactttc tttagacatc gcttggacgg taatttcttt
   113521 attgtttaaa gcaatcaagc ggcttgattg taatccatgt gctaaaaatg cacctgaaat
   113581 agcatttcct gcaatcaatc caataactgg atgtccagct tgacggagtt ttgcataagc
   113641 agcagcacta ttagccaaag ccacatggat accgatcaat tcttcgttat agccgtatgc
   113701 ttggcttggt acatcgatca caacgatgat tggccgtttc acttttttgt ctttatcttc
   113761 ttcgtaaacg tgatccaaaa tttgtcccat gcggaaacct tcaactaaac caacttcacc
   113821 gtttcgtaca cggtacatcg ggtttttagg atcaggtacg attgatgtca cgatggcttc
   113881 ttctatgaat ttacttgatt taccataata cacagtacta atagaagaag ttggattttt
   113941 catgccagtt agtgccgcaa accaatcgta accaacactt gctgcagctg gttcatttcc
   114001 ttctgctact tctggtaagt caattgattt tgcttgatgg ttagcaattg cttcattgta
   114061 atcgtcaatg gccattggtt ttgaaagatc taaagaatca agtaatgata aataaaatgc
   114121 tgagctttca ctgcgatgcg tgtcttttcg ttcgtccatt gctgcgatga cagcagcttt
   114181 gattgcttct acactatcag tcactaattc atcaatgatt tttgattcta cccgttgacg
   114241 agatcctaat gtattccaaa tcaagtcttt gtcactagaa tcaaattctc gaacgcctgc
   114301 ttcttgttca ataacttctg gtccattcaa accgactctc gctttttttg ttgcgattaa
   114361 ataactcatt aatgctgaag taatcgacat accgccaaat gatcctacac gtcctggaat
   114421 caaaccgatg actggtacat attgttttag agcaatgatt acatttccga tttctgaaat
   114481 agataacagt ccgtagtttg cttcttgcaa acgaacacca cctgtatcta gtacaatgac
   114541 tggaaagatc tcatttcctt ttttattttc ttctaacaca tgttcaagtg cagcaacgat
   114601 ttttgcaccg gaaacttcac cgataccgcc accttggaat tttccttcca tagcgattac
   114661 tactgcttgt ttgccattga tcgtgccacg gctgacaacg atcccgtcat cactttcagg
   114721 gacgattcct tgtgctggta aacttggcgc gatgatttga tcgaatggat caatcaattc
   114781 acgtccattg tcaagtagag cgttcactcg ttctctggcg tttaattcta caaaactatt
   114841 tttcatctga aagttcctcc attgcttgcg tcaatcgtag gtacacaact cctggtgtag
   114901 caccgaagtc attgatcacg atatctgctg aaattggata acgatcaaaa aatctcgtta
   114961 atacattgtt ccatacttcg ccgaagccat cgcttcctgt acaaattttt acagtagatt
   115021 ctgttttttc tgttggaaac atcaagattt ccaaatctcc tgaaccgacc actcctacat
   115081 ggattttctt attgatcggc tgtttcgttg gatatttata agctaattgt tccatggatc
   115141 tctccccttt ttgtaaaaca gagcatacac gttctgctta ttcgtctttc ttcttccatc
   115201 tgggtaaatg gcgacaaaag gtttttctcg ccaattttat gaccaagttc tgaatttcat
   115261 tggtggttca tacaggccat ctgaccattc gatcaaatca tcgatggttt gtgcagctaa
   115321 taatgaacgt ttcgcttcac tacgtttgat gcctagatct tctggtaatt tcacgatgcc
   115381 ggctgcacgc aagctatcta gtgattcttt cgtgctagtc attccaagag gtgtaacacc
   115441 gccaattgct gcaattgctg cttgtcgttc ttcttttgtt tttgctttgt acaaataagc
   115501 aattccttct tctgtcacga tatgtgtagt atcttcgcta taaatcatca ctggtgcatt
   115561 ggctaagcct gcttgttttt gaacagctag tgcatctaat tcttcgacaa agactggttt
   115621 tttatttgaa ccatatgttt caaccatttg aacaactaat ttttggcctt tccctaatgg
   115681 atcatcattg tcgcgaagtg attgccatgc tggtgtagaa tgacgacgac cgccaggatt
   115741 atgtcccatg ttaggcgcac cgccaaaacc agataaacgt ccttttgtta cggtagaaga
   115801 gtttccttcg taatctaatt gcaaggttga accgataaac atatcgatgg catattgtcc
   115861 tgcagcttgt gataatgttc ggttactgcg catcgtgccg tctttaccga caaaaaagac
   115921 atctgggcga gcagcaatat atttttccat accaacttcg ccaccaaaac tatggatgct
   115981 ttcaacccaa cctgattcga tggctggaat catcgttgga tgtgggttca atacccagtt
   116041 cttcgcgatt ttccctttta accctagtga ttcaccataa gttggcagta acaattcgat
   116101 agctgctgta ttgaatccaa taccgtgatt caaagattgt acgccgtgtt ctgcgtagat
   116161 tcctttgatt gccatcattg ccattagaat ttgcaattct gtgatgtttt gaggatctcg
   116221 tgtgaataaa gcttctaatt gataaggttt atctgctttc acgacaacgt ctacccagtc
   116281 accagggata tctacacgtg gtaaagaatc gacaacttcg ttgacttgaa cgatcacgat
   116341 accatctttg aaagctgcag cttcgatgat tgttggtgtt tcttccgtat tgaaacctgt
   116401 gtacaagttg ccgtctttat ctgctttatc tgcagctact aaaacaacat ttggaatcaa
   116461 atcgatgaat aagcgtccat aaagttccaa ataagtatgg atatcaccta atttcattgt
   116521 gccgtcttca atcatttggg cgacacgcaa gctttgtgca ccagcataag agaaatcgat
   116581 cttgcttgcg attccttttt caaaaatatt caaatgttct ggtcgtgaga tactagacat
   116641 gatcaaatgc agatcattca ctttttctgc atctactttt tcaagacttt ctgaaagaaa
   116701 acttgcttgt ttttgattgt cgccttctaa tacaactttg tcgccgggag caatcagttt
   116761 ttccattact tctacgattt tttcagtttc tacatacttg ccattgagca agttgctgat
   116821 tttttccaat cgctgcgctt ttttttcgcg tttagaagcc cagttgcggt ttggtttttc
   116881 cataattagt catctccatt tttgatatag aaacgtggcg tcatcaatac catgttggtc
   116941 tagattgatc gtacggatcc ctttatctac accaaatttt gtatgaatca gctttgtcaa
   117001 agtattgccg gggggaaatt caatcgtcag atccattcct aattcttttg ccaagtccat
   117061 catttggttc cactgcacag ggaaagctag gttttccaac aaatctttac gaatctcttc
   117121 tgcgtcctga gtggctcttc catccgtatt ttttatatac aaacatctag catcagatag
   117181 atggacagac tgcatatacg gcttgaattt cttgaccgta tcattcatca agagacagtg
   117241 agatggcaca gggactttca gcagcttcgc tgtttgcgct tggtgccgtt tggcttctgc
   117301 caacgtaacg gataaacctt cgatactccc agataacgta tgttgcatcg gacaattttc
   117361 atttgacaga taaactggat ggtcaggatc aaatgttttt gctacaattt cctcagcttc
   117421 gcttctcgtc acacctacta ctacgcccat tgcataaccg cttggataag cttctttcat
   117481 taagactgcg cggtgataga ctagttccgc tgcattctca aatctcaaag atcgacaagc
   117541 aacagctgca caaaaagcac caatcgaatg tcctgctgca atatcaggct gaataccgtt
   117601 tttttccatt tcatctaaat aaaatactgc ttttgtcagt aatgctagct ggataaaaac
   117661 cgtatcttga tagttttctt ctttatcaac taaccgatac cccgtcacat tttctaaacg
   117721 ttccatgtac atttcaggga tatccgctaa catccctaac ttctgactgc cttgtccagg
   117781 aaataagaaa cctgtactca tgagcctcgc ctcctcagca cttacttttt ccactgctat
   117841 cttagtcaat ttctgctata ataaaaaatg ccaaaaaaag aaaaaaccat aacaaaaagg
   117901 tatggatggg atggataatt atgaaattgc ttcatctgca gtattttaaa aaagtattcg
   117961 aaacaaaaaa tgttacccaa gcagccaaag aattatttat ttctcaacct gccttgagcc
   118021 gtgcgatcaa acatttggaa aacgaattag gtgtcccttt gttttaccat agcggcagaa
   118081 acattgagac gaccgtttat gcagaagaat tttatccata tgcagtcaag acattagaca
   118141 cattagaaga aggaatcaaa ttattgtctt cggtcaatga gaagatcgtt acttccgtca
   118201 tattgtattt agaggtagcc tctgtttcta ttccgaactt ggtccgaatt tttatggaaa
   118261 agcatcctga tatccaatta acgatcatgc aacatcattt accagatgat attaccgatc
   118321 ctgtactgta catcacttcg gagatgaagc ctggcatgac caatattcct atcatgaaag
   118381 aacctgttta tgtcgccatt cctaagaaac atcctcttgc taaaaaagaa catctgcaat
   118441 tagaagatat ccaacgcacc cccatcttga tgctgtcaaa gagcaacgcc ttccgtcata
   118501 cactggatgc agcacttgaa gcgaaaagta tcgaattgac gatcagttcg acgacagatg
   118561 atcctgcgac attacgttct attgtcaagc aaggattagc tgttagcttt ttcccaaaag
   118621 tttcttggtc ttacgataaa aatgatcctt tcgtcttgcg ggaaatcaaa gatttgccct
   118681 tgacacgaac aatttattta tcttctagct tcacagaaga tcatcctttg atcaaaatga
   118741 ttgcaaaaac gatgcgggaa ttttatttag gaaaataaaa aaaaaagagc tgggacaaaa
   118801 ttcttgtcgc agctcttttt tcgaacaaac ggtgttccag aagtaatttc ttagaaaata
   118861 agccgaaatt tcccaaaaat ttgagaagca attttcgtaa attccttctt atttcttgaa
   118921 gctaaacatt tctgtcacaa cctcttctct attgtcctag acattcattg cgttgattta
   118981 aaacttccat caatcaaatc tgctagttca tctagctttt ctattttacg tagatcaata
   119041 gagatccaat gttctttatt catgtgatag gctggaaaat acccctcttt tcctcgcaaa
   119101 attccgccaa gttcaggatc gaccttcaaa tcaagaatat tgatgatcct atggtcactt
   119161 gcatcatcat agattttttc ttctggaaca gacatcagga ccccaaacca ttttttgctt
   119221 ttttgatgtc tgaatacgca ataatcagga aattttttaa aaatatattc tggagacaaa
   119281 ttgtatgttt cattgatgtg atcgattagt tcttttttcg ttagcatctg cttccatctc
   119341 tttcttattg attcgcttta ttcaaagata ccacgtaaga atagaaaaat gaatacaagt
   119401 agttgctaat caaatacttc gatatttttc ttttttatca actcttaaat gttcaatccg
   119461 cctgtgttgg tcttgctacc atctactatt aaaatgttta ttattgtttg cccacctatt
   119521 ttttgatttt agtggtatca tttttcaagt tcatgatgaa attcttatca tcagtaaatg
   119581 ctccagaaaa ataaacaatg attgaaaaat cagttgaaac ttgaccaccg tttattttct
   119641 ccgtattctt agaatagaag ggagtctgat tgatgtattc actaatgaaa aaaatcatta
   119701 ccgaaaaaga tattcgacgt catgtttctt tagtagaaca attgctgaat catacaaaga
   119761 taaccgtaaa tgaattagct gaaatcatag ggacaaccga acgaacgatt ttttctgacc
   119821 ttcaatctat tcgctctcag ctgcccgaag gctgggacat catcagcgat caagcaggaa
   119881 tcagtttgca aaaccagcaa aatttattaa caaatgacct ttgggagatt tttttcaaac
   119941 aatcagtcag tgtcgaactg ctgaaaaatc tgctttttac aaaaaaagtt gctgtccctg
   120001 attttctagc cgaccatggg ctttcttatg ggacattgaa aagacatgtg actaaaatca
   120061 atcagcgttt agcaagttac gacttgcaga tcgatttgac gaaatataca gcttgtattc
   120121 ttggaaaaga gcgtgcgatt cgtacttttt atcatcgatt gctgattcct ttcactcata
   120181 acaactattt tttcgaggat tattcgatcc atgaatccca ttattttcaa tttttaagaa
   120241 acctcagtca aactgaacta gcggttgaaa cagaagagat tttcggaaca tgttggttct
   120301 ttatcaatac gatccgtata aaagcgaatt gccgattaga ttcttctatc cataccaaca
   120361 gcacactctc ctccctgtat gactctgcat tgaaaaaatt gtatttaacg gaaggtattt
   120421 atcttaaaga cactgagctt tcttttgctt ctttctgctt tctggaaagt tggaattaca
   120481 acaataacta tggacaagaa accgctcgct gcctgcatca ttctccattt ttagaggtcc
   120541 tcgaaacgtt tgtagaggaa ctagcttcag agctgtctct tgatcaacta aaaaaaacat
   120601 ctctgactga taacttggct ctcctcatat taaaatatca tgaatcgccg atattgattg
   120661 aagcattgga ccgtcaatac catcgttttt tagaaacata tgatcagcaa tatgcacact
   120721 tgtatcaaca gaaagagaat ttactgaacg atctaaaaaa agaaatagaa atcaatgaac
   120781 caaattactt tctccaactc ttgtctctac tgatccaaca aacgattttt tctatcaaac
   120841 cgaatttgtt caatgtctat tttattttcc aaggagaacc ttcatggaag gcgttcttgc
   120901 agcaggaatt aaaagattac ttaggaaaac gtgtctgctt cttacctatc gaactaaccg
   120961 atctttccac tatttctttt gaaaaaacag atatcttgat ttccaatttc cctctagatt
   121021 ctctagatat ccctattgta tacatttctt ctattccaac gaaaaatgag ctcaatcgtt
   121081 tgactgaact cactttcaaa acttttttat agtaacggct agcgactgta ttctctagct
   121141 gttttttctt ttttgcgaaa cagtttgttc atgatccctt tttctatttc tacaactaag
   121201 aatagaagaa tcacatttgc tccaatcaat aactgttgtg tcatcgttaa acttactgta
   121261 ccgatcagct gctggccgac tggtagaaaa ataaccatcc cttgcaataa agcaaggata
   121321 cctaatgaaa gaaaaagcgc tttgttttgc aaaagacctt ggttcaatga cggcttggac
   121381 aactctctgc aattgatcat gtaaacagct tgagctaaga cgatattttg cagaagaatg
   121441 gtttgctgta agctttgccc ttcaaactgc atagccaata aataggcagg aaccatgatc
   121501 agtagggaga catagatgat ccgaaacaaa tgataaccag ataggattcc ttgctttgga
   121561 tctcttggtg ggcgcttcat gatttctgga tctggcgact cgaaccctag tgcataagat
   121621 aaagtgattg tcgttaccat attgacccat aaaatctgta ctggggacaa aggtagcggg
   121681 cgattcagca taagtgccca aatcaagatc aatccttgag ctaatgcagt tggcaagaag
   121741 aaattgatgg ttttttgcaa attatccaaa atccgccggc cctcttttac ggcttttgca
   121801 atcgtatgga aattatcatc tcctaaaacc atatcagctg cctgtttgct tacttcacta
   121861 cctttgattc ccatagcaat tcctacatcg gatcttttca aagcaggtgc atcatttacg
   121921 ccgtctccgg tcattccgac gatttctcca tttttctgca aagctttaac aattcgcagt
   121981 ttatgttctg gtgtcgttcg agcaaagaca tcgactattg gcacttgctg aatcaactct
   122041 tcatcagaca taagatccaa atcgatccct tctaaaactt tggcagtatg ttttaagcca
   122101 atttgttccg caatcgcttt tgctgtgtct ttgtgatccc ctgtaatcat tttcactgag
   122161 atccctgctt gttggcattc ctttacagct ttgactgcac tttcttttgg cggatcgatc
   122221 aatccggcga tccctgcaaa agttaattgg gttaaagtct catgcgtgag ttcctgtttc
   122281 gaatctactt ttttataagc aaatcctaaa acacgctgac cttttctcgc aaattctgca
   122341 gctgtctgac tccatgcttc ttgttctgca gaagaaagag ttgatttagc aaataatact
   122401 tctggtgcac ctttgacgta aatcaccgct cctttttcct ccgcatgcat cgttgccata
   122461 tatttgtatt cagaactgaa cggaatcttt gcttctattt ttttcaaagg tagattggct
   122521 gtttctgtgt gtcgaatcag cgcaacttct gtcggattgc cacttaaatt ttctgttttc
   122581 tgttcttctt ttgtttttaa ttcctgacag tttttcataa tggacaacac gcaggcttct
   122641 tcttttgcag ctacatccac aactgtcatt tcattttttg tcaatgttcc tgtcttatct
   122701 gaacagatca ctgtcataga acctaatgtt tctaccgaag gaagtccttt gatgatcgcc
   122761 ttttcttttg ccatttcatg tactccaacg gatagaatca tggtcaaaac agctggcaat
   122821 ccttcgggta ccattgacac caccaaagca atcgtagccg aaagaaggat atgccaatcc
   122881 ataccatatc gaaatgtcgt aaaaataact aagaataggc tcaaacaaag aatgccttga
   122941 aaaatttgtt tgtttaatcg gttgattttt ttaatcagag gcgtcgtttg ttgttcgacc
   123001 gattgcaatg cttgattgat tttaccgatt tctgtcgtgt ttcctgtttc tacaacgaca
   123061 ccgactgcag aaccagattg tacaagcgtt cctgaaaaag ccatattttt ctggtcgccg
   123121 atcgattctt cttctgtcaa aacggcatga cttttttcag atgcttctga ttctcccgtc
   123181 aaaatcgatt cttccactaa taaatcatgt gcttcgatta atcggatatc tgctggaacg
   123241 acatctccgg cacgtaagac aatgacatct ccttttacca gttctttcgc agggattttt
   123301 tcttgcatcc cgtttgatag tactatcgct tcttgtccca tcagctgctt caaaccattc
   123361 aatgattctt ccgcttttct ttcttgccag taactaacga gtccattgac gatcactacg
   123421 aataaaataa tacttccttc aatatattca ctagaagcaa atttcaaaaa agaggcaatg
   123481 atcaacacga tcatcagcag atcagtaaaa tgctttgcta tttttttcca tcgttttgtc
   123541 tgctgccttg cttttatttc attatatcct tgatcagtca ggcgtttctg tcgttcttct
   123601 tgcgacaagc cttctgttga tgtttgatag tttttcaata catcttctac cgtcaatctg
   123661 taccagttca tcctatcccc tcctgtttaa ttgatactgt tattatacgc agggaagttt
   123721 tttcaggcgt atcgattatt tcaggggggt ctgaaataat tcgttttgca aaatgtatct
   123781 gggcctaact gatatttcta ttgcttattc ttttatatac aaaatttaag aatacaaacc
   123841 agataaccaa taaaaaagga ataaagcgtg tcgtgctttc gaataacaag ctgaaagcaa
   123901 ttgaagtaaa aaacaccaac aaagcaagat ctttatatct ttgtgtagtg ttccgtttct
   123961 tttctttaat aaattgaaca tgtgcacaca agatcgaaga ccacacaaat agaaaacaac
   124021 tcgttgtcat gcctgccaaa agctgaaaaa acaatcgact atcagctaca aaaaaatgta
   124081 gacaaagacc ggcaaaaaat aacattcctg taattaaaat accgtgtaaa ggtacgcctt
   124141 tcttagaaag cgcacaaaac caatggggca aacttttttc ttttgctgtt tgatataaaa
   124201 tcctgctagt actgtagact gcactatttg ctgaggacaa agaagcaacc aaaatcatta
   124261 gattgacaat tgctgctccg ccttggcaat ggatcacttg cagcatagtc actaaaggac
   124321 tttcttttaa atctaaacaa tgccatggaa caacaaataa aatcgacaaa atcgttccta
   124381 tatagaaaaa gagaatacgc caaggcagac agttgattgc ttttttcaaa ttcttttgcg
   124441 gatcacttgt ctcgccaacg gttaagccga tcatttcaat tccagcaaaa ctgaataaaa
   124501 ccataggaaa tgagactacc attccagcaa agccaaacgg tagtaattca tcgatctgtc
   124561 caaaattttc tttgattccg atagactggg tatgaaaatc tgagacactc aatcctatac
   124621 ccaataagat aaaaaggaaa atccccaaga tcttgatgat tgagaaaaaa gattccaatt
   124681 caccaaacca cttgactgca acaaggttga ctgccattaa gatcattagt gcgctacctc
   124741 cgcttatcaa agctggtaca tgtacaaacc agaaatggat atacaatcct attgctgtta
   124801 attccgcaag tccagctcca agccagcaaa gccaataagt ccaattgaca aaaaatgccc
   124861 actttttgcc taaataatag tcaacgaaat cactgataga gttaaatgta ctatttgcta
   124921 aaaataaatc acccatggcc cgcatcaaac cataaatagc caaaccgcat atcaaataaa
   124981 cgagaataac agctggccct gctgatgcaa ttgcctttgc tgaacctaaa aataatcctg
   125041 ttccaattgc gccacctaat gcgattaact ggatatttcg attagttaga gtccgtttca
   125101 ttgccggttt ttccatcaag ttcacctttt tctttttcaa gcagcatggc gacttcctgt
   125161 tcccaagaag gtttctttat tttttcaata cagagtggta ctacaaacaa aatgacactg
   125221 atggctgtga ctgcaattag attgatccat gttccagctt tcatcgtcag tccgacagaa
   125281 aatccgattg ccgcaaataa aacaataacg acagtccaaa ctccaagatt gctctttttc
   125341 tctttttttc caattctaaa tggtcgattc aactttgcat gtgtataacg caatctcaaa
   125401 actgcgatcc ccatgatgac atagacgata cagtagataa cagtagttga atttacgagc
   125461 atcaaaaatg cttgattgac gccaggaatc agcaaataaa aaagtgcaaa gattgagata
   125521 atgacagact gagtcaaaat aacggatttg gatacgtcaa attgattcgt cttccaaaaa
   125581 ttgaattttg gtggatacgc accttttcta gcactttctg tcactgtctt tgctggtcct
   125641 gaggcccaag cagataattg gactgctaca ccaatgaaga caagtaaact aaacagattc
   125701 acaataagat gtggcaaacc aagaatttgg caataaatcg aaatagattg cgcgatatta
   125761 ttcaattcca tttgtccttt tggtacgaca tttgctacaa ctaaagcatt caaaatattg
   125821 acgaaaaata taaaaattaa cgcggcaaat actccgcgaa tatacgtttt aactggattt
   125881 tccaatcgag tgatatacac agaggacatt tcgataccag taaaaataaa catgatcggc
   125941 gcaaaataaa caaatgagga tgcagtcgta gtatctggta ttaatttgtc ccatgaaaaa
   126001 ctgcctaatg ttccggttgg tacgatccca actttgatcc acgcagcaaa ccctaataag
   126061 agcatcatcg tcaaaggcac gtacaatcct aaccaaacac cgacttttcc gccgattttt
   126121 gccatatcaa atttcaaatt caaaagagtg atgatccaat aaacgactaa aatgacaact
   126181 aatgtaaatt tgctgttcaa accaagcggc acattatcga taatattacc aaacaacgga
   126241 gcgagtgctg aagcgaccat caccatccca ggaaacattt ggacccataa cagccatgag
   126301 acaacgaacc cccatttttt tcctagagca ttcgataccc aaagttctgg accacctttt
   126361 tggggaaaca ttccagcaaa ttcaccagag atcaatgaga ttggaaaagc gtacaataat
   126421 actgcaacca gcatgtaaaa aatcatcgtc cagctcgttg ctgctacatc tggaatattt
   126481 cgtacacttg ctacaagggc gcaggtcatt ccgataaagg tcatggtcgt gatttgcttt
   126541 gatgaaccag tatctttcat ctttattcct tctttcaata gcgacatatg tttttgtttt
   126601 cttttatttt tgttcaagaa cagaaatgac aaggtttcat cgattcagta caaaacccag
   126661 tatcaccgaa acatccattg aacgatctta gtgaactggg tttgatacat gaaacgaacg
   126721 atccaactta tagaacgttt tataaaaaag aatatcttgg cgaggaatat atgaaaaaca
   126781 gggggaaata caattgtttt gatgaagcaa ataattgtct gttctccata tgtctcttgt
   126841 tattctttca cacaaaaatc agttctattg gatgatttcg tttagtttct tttccattgc
   126901 tttggcgatt ttttttgtat aaaaattgaa aattcgatca tcattcaaat aaggagtcat
   126961 gatagccgca cgtagcagag tgattttctg ttcttttttc cattcttctt tcgttagacc
   127021 cattctttca acaaaacgaa tcggagaatc accgtaatct tcttgtgtaa atgtagtatg
   127081 agaagtaata aaccgttcac cataaacatc gccatccata taagaagacc gatcgaacat
   127141 tttttcattt aattcattga tagcgttcaa atctgtacag ccttgttctt tgaataccca
   127201 attgaccata ttaaaatcag gatgattcaa tggatagact tcgattgttt ttcctttaac
   127261 agtaaaagtc agatgatcga gaaattctct gaatctttga gctgcctcga ttgaagcccc
   127321 aatcaattga ccatatccgg tcacattcaa tggcaataca cgatgtgctg tccagactgc
   127381 ggcggcggtt gctccagctt ttgatccttc caaaatataa gctcctaaca tatctggagc
   127441 ctttactgat ttttcaaaca catatggcgc gaaataagaa atgatatttc tcatattttt
   127501 atgtttgatt acgattcctc cagcagcata agggacatag cccatcttat gaggatctat
   127561 cgtgacagaa tccgcttctg aaatagctct gaacccttca tatacctctt tgtcaatcgt
   127621 tacgcagtgg tggaaaacat gatgttcttc aaagaattca gctaaggaag catacggcac
   127681 aaattctcct gcttcgttta gaaacagtga acgcgcatag ccgccatatg cggcatcaac
   127741 atgtaaatag aaatagatcc cttcatcttt taacctttct cttaattgga cgattttatc
   127801 tacactgtct acttgacctt cttctgtcgt tcctacgaca gcaaccacac ctagaattgg
   127861 gattttttgt ccagctaatt cacgaattgt tttttccaaa gcattaatat ccattcgata
   127921 atcctcttga actgggatag caaccatctg atctaagccg actccgcaaa tatctaacgc
   127981 cttcatccaa gaatagtgtt ttgtttgagg gaccagccat ttccctaatc tttggatatt
   128041 ttttccgcta cgggaagaag ctgctttgac ctcatccatt tcttcgtccg taaagctttc
   128101 ggtcatttcc aaaatttctt caacagataa attcaaaagt tcccactcag acattttttt
   128161 cactttttca ggcagcactt ccttgacagc taaaggaatc gatttgatgc aacgggcgta
   128221 ccataatcct tcaagattag caatagaacc atctgctgca atgtgtcccc atccgtctgt
   128281 catattgaat aatgaagcaa aatcctgtcc tacttctgct tccatttgcg aagttgccat
   128341 agaagattcc aatgcaacat tatttgggtt ccacagcatc gcatagttgt aagctaataa
   128401 agcaggcatc aatgtttcag cattcatttg tccccagtaa cggcctgctg aatgccatgg
   128461 gatcgatcct gcgcgtaatc gctgactgac ttcatccaag acctctatca tatggtcgcg
   128521 ggtggctaga taatctggag tgagcttatc tccttcgcta attgcaggca tatctgaagg
   128581 aatataattt tctctccatc caagatgttc atctaccatc ttgttcaaca acattttata
   128641 aactggtcca ttttcagctt tgtcgcctat aaagacggcc ttgatatcca tatctttcat
   128701 ttttcttccc tccatatttt gttggtcaat gtcttgcaga tacatgtgtt tatctgtaac
   128761 acctcttcac tatagcgcca aaaatgagaa attttttttt ataacatcaa atccacgtag
   128821 atttttacaa acaaaaataa ataaagtcgg atttttctga taattcaacg caaaaaagcg
   128881 aacaattttt caataaatca gtcttctaaa atagataacc taaaaaaagc gaacaaacaa
   128941 ataaaatgac tgtccttcat tcactaaaca tatttttatt tttttatatt gttaaaa
//
LOCUS       DARBET010000002       128454 bp    DNA     linear   BCT 07-NOV-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4027
            SAMEA114569841-rid20112183.denovo.002, whole genome shotgun
            sequence.
ACCESSION   DARBET010000002 DARBET010000000
VERSION     DARBET010000002.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA114569841
            Sequence Read Archive: ERR12203666
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 128454)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 128454)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001972618.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001972618.1
            Genome Coverage       :: 132x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/07/2023 13:18:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,041
            CDSs (total)                      :: 2,975
            Genes (coding)                    :: 2,908
            CDSs (with protein)               :: 2,908
            Genes (RNA)                       :: 66
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 67
            CDSs (without protein)            :: 67
            Pseudo Genes (ambiguous residues) :: 0 of 67
            Pseudo Genes (frameshifted)       :: 26 of 67
            Pseudo Genes (incomplete)         :: 28 of 67
            Pseudo Genes (internal stop)      :: 20 of 67
            Pseudo Genes (multiple problems)  :: 6 of 67
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..128454
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA114569841-rid20112183.denovo.002"
                     /isolate="VRE4027"
                     /db_xref="taxon:1352"
                     /country="Denmark"
     gene            212..604
                     /locus_tag="SCJ86_000110"
     CDS             212..604
                     /locus_tag="SCJ86_000110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287002.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
                     /protein_id="HEG1167706.1"
                     /translation="MGEKELRRSMSVFPIGTVMKLTDLSARQIRYYEEQDLVHPERSE
                     GNRRMYSLNDIDMLLEIKDYLSEGLNMAGIKRVYEMAQAKKQEAKNKKPLTDHDVRQI
                     FRDEILAQGGLSKTGHYQSQGLQRQFFD"
     gene            632..1972
                     /gene="glnA"
                     /locus_tag="SCJ86_000111"
     CDS             632..1972
                     /gene="glnA"
                     /locus_tag="SCJ86_000111"
                     /EC_number="6.3.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332748.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I glutamate--ammonia ligase"
                     /protein_id="HEG1167707.1"
                     /translation="MVKKQITGEEIKRIIEEENVRFLRLMFTDILGTIKNVEVPVSQI
                     DKVLENKMMFDGSSIEGFVRIEESDMYLYPDLSTWMIFPWESAHGKVARLICDIYNPD
                     GTPFAGDPRGNLKRALKDMRDLGFTSFNLGPEPEFFLFKLDEDGGITTTLNDKGGYFD
                     FAPTDLGENCRRDIVLELESLGFEVEASHHEVAPGQHEIDFKYADVVDACDNIQTFKL
                     VVKTIARKHGLHATFMPKPLFGINGSGMHCNMSLFNDEGNVFYDKDGELELSETAYHF
                     LGGLLKHARAYTAVCNPTVNSYKRLVPGYEAPVYVAWSGRNRSPLVRVPESRGLSTRL
                     ELRSVDPSANPYLAMAVLLQAGLDGIRNQITPPAAVDRNIYVMDEEEREAAHIQDLPS
                     TIHNAIKELRKDNVMIDALGQHIFSNFVEAKRLEWAAFRQTVSEWEREQYLELY"
     gene            3165..4775
                     /locus_tag="SCJ86_000112"
     CDS             3165..4775
                     /locus_tag="SCJ86_000112"
                     /EC_number="6.3.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000170456.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CTP synthase"
                     /protein_id="HEG1167708.1"
                     /translation="MTKYIFVTGGVVSSIGKGIVAASLGRLLKNRGLKVTIQKFDPYI
                     NVDPGTMSPYQHGEVFVTDDGAETDLDLGHYERFIDINLNKYSNVTTGKIYSEVLRKE
                     RKGEYLGATVQVIPHITNEIKEKIMRAAKMTDSDVIITEVGGTVGDIESLPFLEALRQ
                     MKADVGTDNVMYIHTTLIPYLKAAGEMKTKPTQHSVKELRGLGIQPNILVVRTEQPVS
                     QSVKNKLAQFCDVEPEAVIESPDVDTLYSIPLVLQAQGMDQIVCDHLKLDTPEADMTE
                     WKELEERVLNLKKKVRIALVGKYVELPDAYISVVEALKHSGFAYNSDIEIDWIKAQEL
                     TRENVEERLKDADGILVPGGFGDRGVEGKIEAIRYARENDVPFLGICLGMQMACVEFA
                     RNVVGLEDASSAETNPDTANNIIDLMADQENIENLGGTLRLGLYPCKIKKGTKTAEAY
                     DGADVVQERHRHRYEFNNKYRQLFEEQGLVFSGVSPDNRLVEIVELPEKKFFVGCQFH
                     PELISRPNRPQKLIKAFVGASLADHETK"
     gene            5067..5936
                     /locus_tag="SCJ86_000113"
     CDS             5067..5936
                     /locus_tag="SCJ86_000113"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010753191.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fructose-bisphosphate aldolase"
                     /protein_id="HEG1167709.1"
                     /translation="MPVVSGAEFLKAARKGGYAVGGFNTNNLEWTQAILEAAEAKQAP
                     VLIQTSMGAAKYMGGYKICKDLITDLVEAMNITVPVAIHLDHGDYEAALECIELGYTS
                     VMFDGSHLPFEENLKLAKDVVEKAHAKGISVECEVGSIGGEEDGIIGNGELADIEECK
                     QMVATGIDYLACGIGNIHGQYPENWKGLAFDHLQAIADAVGPDMPLVLHGGSGIPQDQ
                     IEKAISMGISKVNVNTEFQLSFAAATRKYIEAGKDKEGKGFDPRKLLAPGKAAIVEDA
                     KSHIDWFGSANKA"
     gene            6101..7372
                     /locus_tag="SCJ86_000114"
     CDS             6101..7372
                     /locus_tag="SCJ86_000114"
                     /EC_number="2.5.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299679.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylglucosamine
                     1-carboxyvinyltransferase"
                     /protein_id="HEG1167710.1"
                     /translation="MKKIVIKGNRPLAGEVTISGAKNSAVALIPAAILADSPVILEGV
                     PDIQDVHSLIEILEIMGATVHFSNNILEIDPREVVSIPMPHGKINSLRASYYFMGTLL
                     GKFGEAVVGLPGGCYLGPRPIDLHVKGFEALGAEVTNEHGAMYLRTENKGLRGNRIFM
                     DVVSVGATINVMLAAVKAKGQTVIENAAREPEIIDVATLLNNMGAKVRGAGTDVIRIE
                     GVETLHGCRHFMIPDRIEAGTYLALAAAVGNGIKVKNVIFEHLESFIAKLQEIGVHMK
                     ISEDEIEVYPSKELKPANIMTYPYPGFATDLQQPLTALLLMTTGTSEIIDTIYAKRVN
                     HVPELARMGADITVEGNMIIVNGPNKLHGTEVVASDLRAGACLVIAGLLAEGTTTIYN
                     VDYILRGYDHIIEKLTALGASIEMIEEVGAE"
     gene            7369..8664
                     /gene="rho"
                     /locus_tag="SCJ86_000115"
     CDS             7369..8664
                     /gene="rho"
                     /locus_tag="SCJ86_000115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875733.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination factor Rho"
                     /protein_id="HEG1167711.1"
                     /translation="MSDYLTMAELENKTLKEVYSYAKEFKIPYYSKMNKKELSLAVIR
                     AQAEKQGFYYMEGILDIVGQDGYGFLRPINYGPSAEDIYISASQIRRFSLRNGDKVAG
                     KARPPKESERYYGLMHVESVNGKDPEEAKERPHFPALTPLYPQRQLQLETTPQRLSTR
                     MIDIFSPIGFGQRGLIVAPPKAGKTSVLKEIANGITENYPEVELIVLLIDERPEEVTD
                     LERSVKGDVVSSTFDLQPQNHTRVSELVLERAMRLVEDKRDVVILMDSITRLARAYNL
                     VVPPSGRTLSGGIDPAALYKPKKFFGAARNIEEGGSLTILATALVDTGSRMDDVIYEE
                     FKGTGNLELQLSRELSERRIFPAIDIKKSGTRKEELLMPSEELEEIWKLRKNMTGDSL
                     EYTEQLIRFLRKTKNNQQFFKEFQDVSFGKKNPTRNLKR"
     gene            8763..9026
                     /locus_tag="SCJ86_000116"
     CDS             8763..9026
                     /locus_tag="SCJ86_000116"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016174397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type B 50S ribosomal protein L31"
                     /protein_id="HEG1167712.1"
                     /translation="MKENIHPDYHPVVFMDSTTGFKFLSGSTKTSQETVEWEDGNTYP
                     VIRVEVTSDSHPFYTGRQKFTQADGRVDRFNKKYGLKDANAAE"
     gene            9471..9920
                     /locus_tag="SCJ86_000117"
     CDS             9471..9920
                     /locus_tag="SCJ86_000117"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375544.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HEG1167713.1"
                     /translation="MRFDQLIAEELIVFDPSIDSKGSLFERIGDVLKKTNKVRNVQKV
                     VKALFKREEEISTGIEDGFGIPHAKSKYVTKPALCFFHTAKLTDYSGIDEMPIEYIFA
                     IVVPEKSADMHLEILSTLSRRLMNADFRKGIKEATTPADIIRQISQE"
     gene            9977..11395
                     /locus_tag="SCJ86_000118"
     CDS             9977..11395
                     /locus_tag="SCJ86_000118"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HEG1167714.1"
                     /translation="MKIVGVTACPTGIAHTYMSAEKLEVTARNLNHQAKFETQGVKTE
                     NQLSEQEIKEADAIILAVDKEIELDRFAGKKVKRVSTSRAIKEPQVVIDEALRDIGVF
                     VVSNEKEPAANEKKPTIYNHFMNGVNYMLPFVIAGGIIIALSFAFGITAADPNSADYN
                     VLAAAFSRIGGDTAFAMMVPALGAGIATSIAGKAGFAPGIVAGLLASTGGSGFLGGMI
                     GGVLAGYICDFLANKISVKKEFSAIYQLIVVPFLSILIIGLLMVFVIEQPITWVLDAL
                     TNWLNSLGNTSGLLFGLLIGIMMAADMGGPINKSTSTFSIGLMSAGVNAPIAACMAAG
                     MTPPLGIALATLLFKHKFTKEERTSGQSCWILGASYITEGAIPFAVSDPIRVIPSLMT
                     GSAVAAGISMAVGVTSMAPHGGIWVMLIPNVMNNLPMYVLAIASGTVTTAFMLGLLKK
                     TVKEKKQDKWTKNENLTNEVEA"
     gene            11395..12255
                     /locus_tag="SCJ86_000119"
     CDS             11395..12255
                     /locus_tag="SCJ86_000119"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002371432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ketose-bisphosphate aldolase"
                     /protein_id="HEG1167715.1"
                     /translation="MLYTMKELLKVAKENQFAVPAFNICSYDMMKAIIEEAERLEAPV
                     ILEIHPDEIAYLSDEFVAAVRAYAYTSKLPIVIHLDHGGTIEDVLRAIRNGYSSVMID
                     ASLKSFDDNVQITKKVVALAHAVNVSVEAELGTIGNNGSAEGGAETIIYTDPDQAKIF
                     VKETGIDTLAVAIGTAHGLYPKNRTPKLNIDLLKELNQQIDLPFVLHGGSGNPDAEVS
                     EAVKYGVAKVNLSSDLKSVFFNALREILSENPSMYEPNAIYPAANKKVQEVVKHKMEI
                     LNTVGKASLY"
     gene            12429..13202
                     /locus_tag="SCJ86_000120"
     CDS             12429..13202
                     /locus_tag="SCJ86_000120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299683.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR/GlpR transcriptional regulator"
                     /protein_id="HEG1167716.1"
                     /translation="MLNEVENRQQEIVNYLKNNQFARVTDLIELVNYSEATIKRDLVF
                     LEKSGLIRRVRGGAMLIDNRKIDVPYLMKITKLEEETEKRKIADTASSLIRDDMVLFL
                     DSSTTSLHLVRNLARFDGLQIITNGIVTAAMLSEYTTAKVSVLGGAIVPKRATINGSK
                     AYNDALSYYADLAIMSCRGLDFSQGVTETHEGEAMVKKAFRKQSDRLAVLVTQQKIAH
                     KFMHQAIAFHEIDYLVTSKELTKEQYAKVKEHHIECLYG"
     gene            13349..13714
                     /locus_tag="SCJ86_000121"
     CDS             13349..13714
                     /locus_tag="SCJ86_000121"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337649.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2512 family protein"
                     /protein_id="HEG1167717.1"
                     /translation="MKHVKALVVKAIMIWAILWVVLTGLYGVSFMDSTIVGVIIVVMI
                     YVLGDLLILRKVGNIAATIADAGSAAVILWLYLYFMNDTVDIWMKVLIPALLIGVAEW
                     FFHKWLLSQGIVPDERKME"
     gene            complement(13862..15070)
                     /locus_tag="SCJ86_000122"
     CDS             complement(13862..15070)
                     /locus_tag="SCJ86_000122"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326030.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEG1167718.1"
                     /translation="MEKKLFNKYFIGITLINFIVYLIYYLLMVIMAVIAQDTLHASLS
                     QAGFASGIYIIGTLLARLFMGKQLELFGRKFTLRGGAIFYLLSTLAYLYTPTIGALYL
                     VRFLNGFGYGTVSTATNAIVTAYIPASKKGEGINYYGLSTSLAAAVGPFLGMILLNMT
                     DFRFIIWFSIVLVFFVTIACLVFPVKNIPLSEEQKISLRSWNINSFIEKKALFITGIA
                     FLMGLSYSSVLSFLSSYTKVIHLVAAGSFFFVVYALVITFTRPLTGRIFDVKGEQYVM
                     YPSYLFLTAGLFLLSVTTNSVTLLISGALVGLGYGTFMSNGQAVCLKIVREHRISIAL
                     STYFIGLDLGLGVGPYLMGMLKSLTSFRGLYVIAGVIPLICTALYLVNGRKHSHEAEG
                     ELIESLKNEI"
     gene            complement(15089..15367)
                     /locus_tag="SCJ86_000123"
     CDS             complement(15089..15367)
                     /locus_tag="SCJ86_000123"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEG1167719.1"
                     /translation="MNYWMNTIINRLETAYQTRFDMKASLVFLNDAYQNSIELIKAVD
                     ENPTNECEEFLNLFMSTRDLFIRQLVDRYPSNYHDVEVQIQKLKAYSA"
     gene            15521..16216
                     /locus_tag="SCJ86_000124"
     CDS             15521..16216
                     /locus_tag="SCJ86_000124"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Crp/Fnr family transcriptional regulator"
                     /protein_id="HEG1167720.1"
                     /translation="MIFLDESLDGGASLDSRVLKEYLDKNDFPIIRKGYHKYLTFEGM
                     EDSHTYILKEGTVKASVISNDGREFNLRYIKDLEIVSLLKDEYSQFIDSPYNIRVESE
                     YAEFYRINRVRFWQNINQDPKLQHYVKEYYRYRLLYSMKKMQQMLLNGKLGAVCTQLY
                     ELYDLFGVRTEEGMLIDFSITNEEIAKFCGISTASSVNRILKQLKKDGIITVKDHHIV
                     ILNMDLLEDNIIF"
     gene            complement(16658..17455)
                     /locus_tag="SCJ86_000125"
     CDS             complement(16658..17455)
                     /locus_tag="SCJ86_000125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628026.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TPM domain-containing protein"
                     /protein_id="HEG1167721.1"
                     /translation="MMKKFFLFFLLLVSVGWPVATHADTATVNDEAGLFTTDQIQSLE
                     QQAEKLNEKIKGRVLIVTTTSNSEEPRDFADNYLRDAVGNDQNGSVLLLDMGQREIYI
                     STSGNMIDYLTDSRIDSILDDVYDQMTNAAYYNAAKAYLSKSATYVEDGVPGGHYRVD
                     EETGKITRYKVLTTVEIIIAVVLALILSLVFYFVTVSRYQLKSGIYKYPFREKSSIKL
                     TEKTDRLTNSFVTTRRIPKSPPPGSGGGGSTTHSSGGGTFGGGGRSF"
     gene            complement(17452..18636)
                     /locus_tag="SCJ86_000126"
     CDS             complement(17452..18636)
                     /locus_tag="SCJ86_000126"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344032.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TFIIB-type zinc ribbon-containing protein"
                     /protein_id="HEG1167722.1"
                     /translation="MDVLTHKCPNCGGPLTFDPKDQKFHCEYCLNIYTEEEVSQYEQK
                     QKEARMAGEESKEEPSPTEDFTFTAQEQLADMNEAERKAVDEAGGFSDTADESADNTA
                     AIDLFLCPNCGAEIVTDATTAATYCYYCHNPVVLSGRLSGEFLPNKVLPFAVEKEEAI
                     NRFLAWTKKKWFVPKAFFNKDQIDKLTGVYFPYWATDAEVDGAMQANGTVIRIWRIGD
                     IEYTETKQFAVSRAGKLSFKELVKNALSKNTQQKMVEGVQPFPLDKAIDFKSQYLAGF
                     QAEKRDIEYEAIKSQIQSELKDYSEKLLKDTANGYTTLTGVRTSASITNEVNNYVLLP
                     VWLVTYRSNDSSKKVYYYAMNGQTGKVSGVLPISQKKLGLTALGIFAGLAILFLIGGY
                     LI"
     gene            complement(18661..19908)
                     /locus_tag="SCJ86_000127"
     CDS             complement(18661..19908)
                     /locus_tag="SCJ86_000127"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338701.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SPFH domain-containing protein"
                     /protein_id="HEG1167723.1"
                     /translation="MGLIKAATSMVGGGLADQWLEVIEPDNMSDTTVMTKGVKVRKDD
                     KRGSNRKGTEDVLTDGTVVHVYPNMMMLLVDGGKIIDYTAEEGYYTIKNDAAPSMFNG
                     TLKEAIAETFDRFKFGGVTPQKQQVFYINLQEIKGIKFGTSAPLNYFDNFYNAELFLR
                     AHGTYSIHVVDPILFYTNAIPKNKTQVEINDINEQYLAEFLTALQSAINQMSADGQRI
                     SYVPSKSLELSKYMDTALDDSWRELRGMEIVSVAVASISYTDDSVKLINMRNEGAMLG
                     DPSVREGYVQGSIARGMEAAGKNEAGAMTGFMGVGMGMNANGSYLSQAAQNNQEQIKQ
                     QAEKQNQQTAQGNADTWTCPVCGTENSGKFCSNCGAAKPVENVQPKLQMRCSECNEIV
                     DLSNGIPKFCPNCGKPFKGIPVN"
     gene            20123..21868
                     /gene="dnaX"
                     /locus_tag="SCJ86_000128"
     CDS             20123..21868
                     /gene="dnaX"
                     /locus_tag="SCJ86_000128"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315431.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit gamma/tau"
                     /protein_id="HEG1167724.1"
                     /translation="MAYQALYRVWRSQRFEDIVGQKAVTQTLKNAIVSHKTSHAYLFT
                     GPRGTGKTSAAKIFAKAINCPNSKDGEPCNECEMCRSITAGTQEDVIEIDAASNNGVE
                     EIRFIRDRANYAPTKAAYKVYIIDEVHMLSTGAFNALLKTLEEPKESVIFILATTEPH
                     KIPATIISRTQRFDFKRINTADIVEHLEFILKESTISYEPAALNVIARSAEGGMRDAL
                     SILDQAISFSEGTITLDDAMQVTGSLTDEMMDSYLSACVKKEVPEALETLNQILAAGK
                     ESRRFLEDMLQYCRDLLMYQQAPNLVEEQSSTIRSTFTELAQTVSADQLFSMIKILSD
                     TQNEIRFTNSAAIYLEVATVKLAQLPSASVPSADVAFADDQIVKQLQQEIKELRNEIK
                     ELRSNTGIENTKVQPVQQTNHKKQTSTYRVPTERVYKVLREATRQHLNQVKEVWEDLL
                     MSLSVTQRAMLKASEPVAAGPTGLVIAFDYEIVCQRAANDEELQLSVHNNLSRMIKDY
                     APDPVYITRESWPELRRNYLTQAQEEGTENSFASEDSEGEIELLPQEQEVPEETLVVK
                     AEELFGSEAVTVVDD"
     gene            21888..22202
                     /locus_tag="SCJ86_000129"
     CDS             21888..22202
                     /locus_tag="SCJ86_000129"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbaB/EbfC family nucleoid-associated protein"
                     /protein_id="HEG1167725.1"
                     /translation="MMRGMGNMQGMMKQVQKMQKEMAEAQELLNKTEFTGKATNELVK
                     AVFTGDRRMKDIQINEEVVDPDDIEMLQDLVVMAVNDAIAQIDVETEKKLGKYAKGLP
                     GF"
     gene            22227..22823
                     /gene="recR"
                     /locus_tag="SCJ86_000130"
     CDS             22227..22823
                     /gene="recR"
                     /locus_tag="SCJ86_000130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329007.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombination protein RecR"
                     /protein_id="HEG1167726.1"
                     /translation="MQYPEPIAKLIDSYMKLPGIGQKTATRLAFYTIDMKDEVVNEFA
                     KSLLSVKRDLHFCSICGNITEEDPCEICRNPSRDRSVILVVEEPKDVMAMEKMREYHG
                     LYHVLHGVLSPMEGTGPEDINISSLLQRLHDADVKEVIIATNATTEGEATAMYLSRLI
                     KPAGIKVTRLAHGLSVGSDIEYADEVTLLKAVEGRREL"
     gene            22887..23531
                     /locus_tag="SCJ86_000131"
     CDS             22887..23531
                     /locus_tag="SCJ86_000131"
                     /EC_number="2.7.4.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327184.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dTMP kinase"
                     /protein_id="HEG1167727.1"
                     /translation="MNGLFITIEGPDGAGKTSILNELYPLLKEVAKTEIIQTREPGGI
                     PIAEEIRAVILDPKNDRMDERTEALLYAAARRQHLVEKVLPALAKGKIVLCDRFVDSS
                     LAYQGAGRRIGVTEIARLNEFATEGTTPDFTLYLDVDSDTGLRRIKKNRQNQIDRLDS
                     EGLEFHQRVRHAYLKLAEENPERIHKVDARKSFEEVLQTSYHTIIEQYPQFFEN"
     gene            23546..23875
                     /locus_tag="SCJ86_000132"
     CDS             23546..23875
                     /locus_tag="SCJ86_000132"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005878277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic-di-AMP receptor"
                     /protein_id="HEG1167728.1"
                     /translation="MKIILAIIQDKDSNRLSNELIDANIRATKLSSTGGFLKAGNSTF
                     IVGIEDDRVEEALEIIKKTCESRKQFVSTPVTLDISMDGGVPYPVEVEVGGATVFVLP
                     VEGFHQF"
     gene            23889..24827
                     /gene="holB"
                     /locus_tag="SCJ86_000133"
     CDS             23889..24827
                     /gene="holB"
                     /locus_tag="SCJ86_000133"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300512.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit delta'"
                     /protein_id="HEG1167729.1"
                     /translation="MNQEINLAQMQPIVYQQLQRSFEHGRIAHAYLFEGEKGTGKHEV
                     GIWLAQHLFCTQMKDNLPCGKCNNCQRIEKKEHPDVLVIEPEGQTIKVDQIRRLQTEF
                     SRSGYESRKKVFLIKEAEKMNSSAANSLLKFLEEPPGDFLAVLETDAVGRILPTIQSR
                     CQVLHFQELSKEALIHRLQEEQIPLEKAKLLAFLTNSLGKAVEISQDEWFNDAKDLIY
                     QWFVYLQKQDTQAFIYVQKKLVKSFKEKTQQFTGLSVLMFYYQEAQRQAVANGEYGKV
                     KQINQIIERILIAEQKLRSNVSFQAVAEQFVLHTIF"
     gene            24863..25687
                     /locus_tag="SCJ86_000134"
     CDS             24863..25687
                     /locus_tag="SCJ86_000134"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332104.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stage 0 sporulation family protein"
                     /protein_id="HEG1167730.1"
                     /translation="MVEVVGVRYRDAGHIYYFAPGDSEYVYSDKVLVESQQSLHIATV
                     VLPKKEIDPNDLPEAIRPILHKASQAELEKDKKNKKDAYDALKIAKEKVQLRELEMKM
                     VKAEYTFDRNKLIFSFTADGRIDFRELVKDLAAVFRTRIELRQIGVRDEAKIIGGIGP
                     CGRPLCCSSFLGDFVPVSIKMAKDQSLSLNPTKISGLCGRLMCCLQYESEEYETAKKE
                     LPDFGKVIATPDGKGKVVGLNLLSRIVSIRLTGRENPVEYEWDELKEFIQVGEMNG"
     gene            25680..26027
                     /locus_tag="SCJ86_000135"
     CDS             25680..26027
                     /locus_tag="SCJ86_000135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003127096.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA replication initiation control protein YabA"
                     /protein_id="HEG1167731.1"
                     /translation="MDKRSLYDGLNQLESELRNTLTELVEMKHALHEIVEKNTTLEME
                     NQRLREHLRELNQVAQAPAENVKQELSKSRMNLEKIYEEGFHVCYDLYGSRRDNDEPC
                     AFCLEVIYRESGR"
     gene            26096..26968
                     /gene="rsmI"
                     /locus_tag="SCJ86_000136"
     CDS             26096..26968
                     /gene="rsmI"
                     /locus_tag="SCJ86_000136"
                     /EC_number="2.1.1.198"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346306.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA
                     (cytidine(1402)-2'-O)-methyltransferase"
                     /protein_id="HEG1167732.1"
                     /translation="MYNQKSFKGNPVYGDLYLVPTPIGNLEDMSYRSVRMLQESDLIA
                     SEDTRNTQKLLNHFEIHTPQKSLHEHNYKERVPQLIALLKEGKTIAQVSDAGMPSISD
                     PGHELVLACIKEGISVISIPGPTAGMTALIASGLVPQPFLFYGFLPRKKKEQKETLKK
                     LKEQTVTLIFYESPYRITATLTNFLEIFGNRQVVLCRELTKVHEEYLRGSTEELLAYI
                     EEHPVKGECCLLVEGNLFEMPVTEVDEELGTLKEQVEAKIASGEKPNAAIKAVALKNG
                     LKKQEVYRQYHELD"
     gene            27077..28198
                     /gene="dinB"
                     /locus_tag="SCJ86_000137"
     CDS             27077..28198
                     /gene="dinB"
                     /locus_tag="SCJ86_000137"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase IV"
                     /protein_id="HEG1167733.1"
                     /translation="MMELQFPLHNDTSRKIIHIDMDAFFASVEERDHPEFRGHPLVIA
                     RHPSDTGGRGVVTTANYEARKFGIHSAMSAQKAYELCPQAIFKPGDHHKYSDISKQVR
                     EVFYRYTDIVEPVSIDEAYLDVTENKINCKSAIKIARMIQADIWESVQLTCSAGVSYN
                     KFLAKLASDYQKPRGITVVAPQDAVPFLKALPIEKFHGVGKKTVPRMHELGIYTGKDL
                     YECTEMMLIRNFGKMGYSLYRKVRGIHDSPVNVTRERKSVGKEHTYGQPLQTEEAVLT
                     QLRQLAEKVEEALRRVQKHGKTVVLKVRYTDYSTVTKRVTLPEYIYKKEALFYQASLI
                     WEEILGVEKGIRLLGITLTNLDPMTYENIVLPLWENQEI"
     gene            complement(28252..28854)
                     /gene="nrdG"
                     /locus_tag="SCJ86_000138"
     CDS             complement(28252..28854)
                     /gene="nrdG"
                     /locus_tag="SCJ86_000138"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005878087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic ribonucleoside-triphosphate reductase
                     activating protein"
                     /protein_id="HEG1167734.1"
                     /translation="MRNPKPKEWLAKDYSQNYIADYKPFNFVDGEGVRNSLYVSGCLF
                     ACEGCFNKAAQNFRYGKPFTPELEEQIIADLRNDYVQGLTLLGGEPFLNTDVCLQVVR
                     RIRKEFGTTKDIWSWTGYAFDELLQDSEDKLELLSQIDILVDGRFELSKRDLKLQFRG
                     SSNQRIIDVQKSLESNQVVIWEKCTDATETYEQIKKQDLI"
     gene            complement(29169..31358)
                     /gene="nrdD"
                     /locus_tag="SCJ86_000139"
     CDS             complement(29169..31358)
                     /gene="nrdD"
                     /locus_tag="SCJ86_000139"
                     /EC_number="1.17.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002391658.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic ribonucleoside-triphosphate reductase"
                     /protein_id="HEG1167735.1"
                     /translation="MKVNYEEKTVVTQQPETSQMKVIKRDGRHVDFDEQKINEALIKA
                     EKKIHGSLSPLTYEKIQMIADLVVQEIKSRFAENVKIYEIQNIVEHILLEKNEYELAE
                     EYINYRTKRDFARSKATDINFSIEKLINKDQTVVNENANKDSNVFNTQRDLTAGIVGK
                     SIGLKMLPPHIANAHQKGDIHYHDLDYHPYTPMTNCCLIDFKGMLNNGFKIGNAEVES
                     PKSIQTATAQISQIIANVASSQYGGCSADRTDELLAPFAELNYKKHLKDAEEWIDSPE
                     RQKEYAKAKTKKDIFDAMQSLEYEINTLFTSNGQTPFTSLGFGLGENWFEREIQKAIL
                     QIRINGLGSEKRTAIFPKLIFTLKKGVNLNPEDPNYDIKQLALECATKRMYPDILNYD
                     KIVELTGSFKVPMGCRSFLQGWKDENGQEVNVGRMNLGVVTLNLPRIAIESKGDQNKF
                     WQLLSDRLEIMKDALLYRVERCKEAIPANAPILYMYGSFGKRLSRTDSVNELFKNRRA
                     TVSLGYIGLYEVASAFFGGEWETNPEAKAFTLDIVKELKANADAWGDEYGYHFSVYST
                     PSESLTDRFCRLDTEKFGIIENITDKEYYTNSFHYDVRKNPTPFEKLEFEKDYPKYCS
                     GGFIHYCEYPMLQQNPKALEAVWDFAYDKIGYLGTNTPIDHCYQCGFEGDFKPTERGF
                     ECPECGNHDPKTCDVVKRTCGYLGNPQARPMVHGRHKEISSRVKHMK"
     gene            31657..32451
                     /locus_tag="SCJ86_000140"
     CDS             31657..32451
                     /locus_tag="SCJ86_000140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318288.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEG1167736.1"
                     /translation="MKNERGGEELEKMIEVKHLSKSYGSKLSPVKALDNISFTVEEGE
                     FVGIMGPSGAGKTTLMNILSTINLPTMGSVSIQGKEITKMKNSELSDFRRKKLGFIFQ
                     EFNLIDTLNAKDNILLPLAVERMTKEEMEKRVRHVAQLLNIEELLKRYPDELSVGQRQ
                     RIAAARALVVQPQVIFADEPTGSLDSKSATELLNYLKTMNQKEKATILLVTHDPYTAS
                     YCDRILFIKDGVIFSEVVRRGTRREFFEKVIDMQATIGGGGKMNAV"
     gene            32441..34483
                     /locus_tag="SCJ86_000141"
     CDS             32441..34483
                     /locus_tag="SCJ86_000141"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEG1167737.1"
                     /translation="MLYKLAVKSFFRQSRGYLVYFFSLTLSVMIYYSFSAMTYDQSLV
                     RRASQDTSIDGGLSFGGFIITVVLLFFVFSANRFFLNRRQKEIGIYQLFGMNKLQISG
                     IYVLEIMIIGLFACISGILLGIIFSKLFSMILVRMMDMDLTSPFFISIPSVVDTLFAF
                     FIILLAVSVYSIWKIWRYPMIRSFGEKEQADSSTMRFRTRHRLLAVIGLLLLTSGYFG
                     ASHFREITSWMISYHYLDTLVFFFPFLIFFVCVVGTYLFFCYTFRLFLIILSKWKIYY
                     YGINFLIMGNTQVHLLKSWRTNSLITVILGISLMTIGGTASISTILSYQEKVSSPMDY
                     QFDVETEKKIKPILAEEGQKITKEITLHYKVIGSIYSQNLEEPMFQTANLITETQYQA
                     FRKVNPDLPNISLKSPGHTVLLDQMQTLFRNISLYGSSIGLPDNQELKIQQMLPSVLG
                     DETMVYLSPVLIVDDTVFEKAKGVDYQVVNVDVSGGNNEKIDERLSNDLSIKWLNAVY
                     YSYDIVNGSLQGEISTKKLPDDQMKEAQFSQESSRLNFSSSYSKLRAVNRRIGINIFV
                     TLFVGMIVIIATGSILTVRQLAEAEEEKENYHLLTKLGIPQRRIRRMIFEQNALTFFP
                     PMILGITHAVFAINVFTQYIKTADYWLAYLVSGLLILIYLLLYVITSQLYCRIIEE"
     gene            34764..34913
                     /locus_tag="SCJ86_000142"
     CDS             34764..34913
                     /locus_tag="SCJ86_000142"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338698.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="teichoic acid D-Ala incorporation-associated
                     protein DltX"
                     /protein_id="HEG1167738.1"
                     /translation="MNKWRSNPKLNYWGKFIIRTIFYSAVLLFLIYLYHYKNIQGGTF
                     IYNEF"
     gene            34924..36438
                     /gene="dltA"
                     /locus_tag="SCJ86_000143"
     CDS             34924..36438
                     /gene="dltA"
                     /locus_tag="SCJ86_000143"
                     /EC_number="6.1.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339604.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanine--poly(phosphoribitol) ligase subunit
                     DltA"
                     /protein_id="HEG1167739.1"
                     /translation="MEIKTIIEAIDDWGINEPDRVAYQADETHTFGELKQASDALAYY
                     LEKKVEGDGPIVVFGNLEFEMIVSFLGVVKSGHAYLPIEEHTPKERILSIFRVAKPSM
                     VISIGDWIEDIPSVPVITKKEFQEIKKIPVGFYPRNSVKGTENFYIIFTSGTTGEPKG
                     VQISHDNLVSFVQWTMEDFGIKPGMHFLAQAPFSFDLSVFSIYPALVSGGMLKPLNKA
                     VVQDFRQLFATLPALKLNVWVSTPSFMDICLMEPTFNGENVPELDMFLFCGEELTKKT
                     AESLLDRFPNARIYNTYGPTEATVAISSIQIDQHVLDSYDRLPIGYVKKDTKVSIVQE
                     GKPVSKGETGEIIIAGPSVSKGYLNNPEKTAAAFFCQNDLASYHTGDAGRLDEYGLLF
                     YEGRMDQQVKLHGYRIELGDIEHYLLQDNRIKQAVVVPKYQGTKVQQLVAFVVLENKT
                     KQPDFQLTKSIKEQLLEVVMDYMIPQKFHYVDLLPQTVNGKIDRKKLTAEVNPE"
     gene            36435..37640
                     /gene="dltB"
                     /locus_tag="SCJ86_000144"
     CDS             36435..37640
                     /gene="dltB"
                     /locus_tag="SCJ86_000144"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336916.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanyl-lipoteichoic acid biosynthesis protein
                     DltB"
                     /protein_id="HEG1167740.1"
                     /translation="MMLPHMIPYAEPFYFFLLAIALVPIVLSLLIWEKRLPVYQSAVT
                     LFFLFVSFGGNYWQQGAALIGYVLWQTILVWGYFVYRQRKNQTGWFFAAVILAIIPLF
                     LTKVTPFFEHGKASLLGFLGISYLTFKSVQMIMEIRDGMIKTYKPFRYIQFLLFFPTI
                     SSGPIDRYRRFEKDQLHPPVRDKYVDLLGKGVQNIFVGFLYKFIIGYYFGQVLLPVVG
                     RIALQHGGISWALVGYMYVYSMYLFFDFAGYSLFAVGTSYMMGYQTPINFNKPFLSWN
                     IKEFWNRWHMTLSFWFRDYVYMRLMFFMMKKKFFKSKIVMSNIGYFALFLLMGIWHGL
                     TWYYIVYGLYHACLICLTDAWIRFKKKHKEQLPSNKFTHGLAVFLTFQAVCFSFLIFS
                     GFLDKLFFS"
     gene            37685..37918
                     /gene="dltC"
                     /locus_tag="SCJ86_000145"
     CDS             37685..37918
                     /gene="dltC"
                     /locus_tag="SCJ86_000145"
                     /EC_number="6.1.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310246.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanine--poly(phosphoribitol) ligase subunit
                     DltC"
                     /protein_id="HEG1167741.1"
                     /translation="MEEQILSILEEITGTDEVRKNRDIDLFDEGLLDSLASVQLLVEL
                     DGELDIQVPVSEFEREDWSTPNKIIQQATALKG"
     gene            37921..39189
                     /gene="dltD"
                     /locus_tag="SCJ86_000146"
     CDS             37921..39189
                     /gene="dltD"
                     /locus_tag="SCJ86_000146"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299605.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanyl-lipoteichoic acid biosynthesis protein
                     DltD"
                     /protein_id="HEG1167742.1"
                     /translation="MKKKLFAIFGPVVLAVGLLFVFFTSSYQVDISNPETIRRASSSM
                     SVNVLKGDAIKNYALSEKQYIPFFGSSELSRISPFHPSVLAEKYQRNYRPFLLGAPGT
                     QSLSQYMMMRSAGDAMKNKKVVFIISPQWFVKNGVKTDYFNTYYSELQTYDWLFSMKK
                     VTPADRYLARRLLTFSKVKENDTLTAILQTIKKGKLPLPESLNQLRSQWNMLKREDEV
                     FSNIGLKDRQQKIDHESKRLPKQYQETELSILANQIGERETTNNPFGLKNDFYTHRIR
                     AHEPELKQSQKNWDYRFSPEFSDFQLVLDQLAKNHNEVLFIIPPVNEKWSDYTGLSQE
                     MLQGFAKKIKFQLNSQGFNRIADFVNQAGTNYFMEDTIHLGWKGWLAADQQIRPFLEE
                     NHITASKYHLDDAFFSKSWQHQIPDKLQLK"
     gene            39330..40412
                     /locus_tag="SCJ86_000147"
     CDS             39330..40412
                     /locus_tag="SCJ86_000147"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628031.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M42 family metallopeptidase"
                     /protein_id="HEG1167743.1"
                     /translation="MDTKEEQLLIDLTSAKGVPGNEEEVREVFREYAKPFADDIFYDG
                     LGSVIAKHGAGGGPKVFISGHMDEVGFMVTKITEKGFLEFQTLGGWWGQVMLAQQVEI
                     KTREGKIVHGVIGSKPPHVLTPQVRNKPYEIKDMFIDIGASSQEEAKEWGIRPGDMVT
                     PYIEYKRMNGSKYLLAKAWDNRIGTAVSLRVLENLSKEAHPNVLFAGSDVQEEVGLRG
                     ARTSTHLVNPDIAFALDTGTAGDTPGMTPKEADSILGKGPQILIFDASMIPHKKLLNF
                     VIDVAEEMDIPFQYTVISGGGTDAGQMHLTRDGVPSVAITVPVRYLHSHTSVIHEDDY
                     LNTVKLVTELVKRLDSETVEKIRSYE"
     gene            40412..41215
                     /locus_tag="SCJ86_000148"
     CDS             40412..41215
                     /locus_tag="SCJ86_000148"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288098.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF975 family protein"
                     /protein_id="HEG1167744.1"
                     /translation="MNNRIPISEYRKKARVSLENQWGINTWLVFLSIIFTSVIQNFFD
                     GFSPFPEESMQQHLLSFLVDNLLLFAFNYALFYIALVVVRGGRPKSNLLFSVFQRKYF
                     GSILGINLINSVINWLLNSLIFLPMFLISGVSTYMQLLFSSNAVPTGVMPEDFLDISF
                     VLSMIGMIFVSALIMSIVGGLFQFAAWTKFDYPDLSVIQCLKYGWFLLKDRIGTYILL
                     QLSFIGWYLLGAIALFIGLLWVIVYVNVSVAEFYEQARIEKGGPDAYFT"
     gene            41285..42025
                     /locus_tag="SCJ86_000149"
     CDS             41285..42025
                     /locus_tag="SCJ86_000149"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341957.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HEG1167745.1"
                     /translation="MKLTVLGCLGAYPYKNQGTTGYLLQSDGFNLLIDAGSATLIKLQ
                     EHLDPLDLDAVILSHYHHDHIADLGVLQYYWQLHPKRNPETLLSIYGHTKDSIHFEEL
                     TLENVSKGHPYFEAEELKIGPFLITFMETIHPVVCYAMRIVEETTGKVFVFTGDSGYL
                     ESFADFAKDADLFLADTYLFEGNEHHHAHFTSKEAGELAKSANVKKLVLTHLPQFGDL
                     EQLKNEAQKAAGKGIDIELAAVDKVFNI"
     gene            42036..43040
                     /locus_tag="SCJ86_000150"
     CDS             42036..43040
                     /locus_tag="SCJ86_000150"
                     /EC_number="6.3.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631995.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoate--protein ligase"
                     /protein_id="HEG1167746.1"
                     /translation="MIFVPNENHDPRINLAIEQYLLQEMPLDEPILLFYINEPSIIIG
                     RNQNTLEEINRDYVEENGIHVVRRLSGGGAVYHDFGNLNFSFIMPDDGDSFRDFAKVT
                     QPIIQALHELGVAGAELKGRNDLVIDGMKFSGNAMYATNGRMFAHGTIMFDSDINEVV
                     NALKVKKDKIESKGIKSIRSRVTNIKPFLPEEKQKMTTEEFRQEILLKIFGVESVEEV
                     KTYELTDEDWKKINRISEEYYRNWDWNYGKSPDFNFSRQKRFSIGSIEVHLNVSEGMI
                     KEAKIFGDFFGLGDISDVEKKLVGQKYDKASLSKIVSQIDVKKYFGAIEPEELLTLLY
                     "
     gene            complement(43085..44929)
                     /locus_tag="SCJ86_000151"
     CDS             complement(43085..44929)
                     /locus_tag="SCJ86_000151"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631160.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEG1167747.1"
                     /translation="MSFFNNIYNKKESFYLRIKWSIIIILSLLFLTGIAYFFYFIFSP
                     ADSNQMKEIKDSWVFYTKDDPDFKFDSKYTRTIPTVDKDETFIMETTLEKPLDEANLL
                     IKGNHQWITVYLGEKILYQRKDYQEESNPGLSLAVIDLPANYIGKKLTLTVSSPYQNY
                     AGLPPKVYLGTTGPLISFIYSQSVPQVLTMIIAVFIAIGTFFYTIYLMYKQKRLEYSL
                     ILLSCFALALGFEAVSEDILSGILFEPFVHSFLSHLFIILTSAFIICYYWSRMFYYKK
                     WYGIFCTFQLLIFIGVLLYATFTPAELPELMPIANIASVISTLVTSLACIGEAYKNNR
                     FYVACTPWIVLVAIIHCLIYIQTALGIYQTTINWSTILYIIILIVIISYNLIDHILNT
                     DKDRRQVDFLKIKNDLLEQHYEQLRQHIQEVNTLRLEFVQEMENLQDLMHTDQTKAKN
                     YVEKILEEAKNFEMLCSFCNHQVTNLILARYQKLAAKRNIQITFQADLPEELPINDDD
                     LAQLLIHALEHSFRETHAIENPLYRKIYLSIHEQEEHFVICCEHSAHYDTNLFSRGIT
                     EELDDQERFDLMMMKDVADRYTGTLTQEKDTFIDRITVQLSRNYPNPV"
     gene            45141..45659
                     /locus_tag="SCJ86_000152"
     CDS             45141..45659
                     /locus_tag="SCJ86_000152"
                     /EC_number="3.4.21.89"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294028.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptidase I"
                     /protein_id="HEG1167748.1"
                     /translation="MPDYKKITFISSITLIIFLILILLVVLVPRIFGLTPYIIEDKEM
                     SPYYAQGNLIYVRPKNTNEILVGDIITYYENSGKKISTRRVIAVENNHEDFYTKADTK
                     TYIEPGVVKKRNIIGSPIFQIPYIGLVLSKTAFAWIQGLFFTIAFCLTGITAWNTFVE
                     LKKKRRRTQKFL"
     gene            45787..46470
                     /locus_tag="SCJ86_000153"
     CDS             45787..46470
                     /locus_tag="SCJ86_000153"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343050.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="O-methyltransferase"
                     /protein_id="HEG1167749.1"
                     /translation="MRNEMMHRPVVKEELLDFMRTQQKKLPGELGKLEEEAHVAEVPV
                     IPHETVVFLKFLLGQLQPERILEIGAAIGFSSSVMATTIGENAHVTTIDRFDVMIEKA
                     KKNYERLGLTDKVTLLEGQAADILPELSGPYDFIFMDSAKSKYIEFLPECLRLLRKGG
                     VLMVDDIFQGGTILLPDEEIPRGKRAIHRKLNEFLRVVMDHPDLTSTILPLGDGVILM
                     TKESETIEL"
     gene            46779..46852
                     /locus_tag="SCJ86_000154"
     tRNA            46779..46852
                     /locus_tag="SCJ86_000154"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:46813..46815,aa:Arg,seq:tct)
     gene            47011..47370
                     /locus_tag="SCJ86_000155"
     CDS             47011..47370
                     /locus_tag="SCJ86_000155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016922519.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1304 family protein"
                     /protein_id="HEG1167750.1"
                     /translation="MTILSRILVTLVAIEFFYIMYIETVRTDSDTTSRVFKMSKEELS
                     RKSVQTLFKNQGVYNGLLGVGLLYGAYLSSASKEITSMLLISIFFVALYGSLTSDRWI
                     LLKQGGLALLALLSIFL"
     gene            47493..49124
                     /locus_tag="SCJ86_000156"
     CDS             47493..49124
                     /locus_tag="SCJ86_000156"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SulP family inorganic anion transporter"
                     /protein_id="HEG1167751.1"
                     /translation="MFKNYVSSLKKEFSGYDLSRFQKDLLAGITVAAVALPLALAFGV
                     SSGADAAAGLITAIIAGIVIGGLSGGFYQISGPTGAMAAILMSIAATKGMQGVLLATF
                     LAGLFLLAAGLFRLGTLTSFIPAPVITGFTSGIAIIIALGQIDNLFGVHSEGTNVIEK
                     LSSYQTIGFPINTASLIMGGLVILGMFIYPKKWAKRMPSSLLAIILATAVMLLFHLPV
                     ATVGKIPQTLISSNRLNMGDFSFSALQQVAVPAISIALLGMIESLLCGASAGRMANKP
                     LDSNQELMAQGIGNLLLPFFGGIPATAAIARTSVAIKSGAQTRLAGMIHAVVLLLSML
                     VFAPVMSQIPMPALAGVLIVTAWRMNEWETIKELFTNKYWSAVLLFILTMICTVIFDL
                     SIAIVIGVIGGCVFFVVKSAAITISLETIDWQRMGLPETEKLNNWTVVYISGPLFFMS
                     SEKLKNKLEELNHHDGVIFSMRGVPSIDLTARAVFDEFQEKAEQRGQTIIYTSLQPEV
                     EKQLSHLWEHTFTHQHQTVAHALTALHAKYTAISH"
     gene            complement(49186..49827)
                     /locus_tag="SCJ86_000157"
     CDS             complement(49186..49827)
                     /locus_tag="SCJ86_000157"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738861.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uracil-DNA glycosylase"
                     /protein_id="HEG1167752.1"
                     /translation="MEYPKELIQEASLRMNGRPVEGFIAGQGPLHPKLMLVGEAPGKT
                     EIDTHVPFSGQAGKELMQALSSTGLTREEVYITSAVRSRPYRVTHRINKRTQQSETVY
                     PNRTPTRSEVFAHAPILDYELMKVQPKLIATLGNIGLQRLLGKEYTVTKNHGQLYTGP
                     VLQLTEQKDAYEWSEKNYRILPLFHPAAIFYNRSLVPVIHQDWQVLASLLQQY"
     misc_feature    50013..50089
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00559"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="ribosomal protein L21 leader region; Derived by
                     automated computational analysis using gene prediction
                     method: cmsearch."
                     /db_xref="RFAM:RF00559"
     gene            50109..50417
                     /gene="rplU"
                     /locus_tag="SCJ86_000158"
     CDS             50109..50417
                     /gene="rplU"
                     /locus_tag="SCJ86_000158"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002355866.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L21"
                     /protein_id="HEG1167753.1"
                     /translation="MYAIIKTGGKQVKVEVGQAIYVEKLDVEAGEKVVFDEVILVGGE
                     STKVGAPTVAGATVEGTVEKHGKQKKVVTFKYKPKKHSHRKQGHRQPYTKVVIDAINA
                     "
     gene            50444..50788
                     /locus_tag="SCJ86_000159"
     CDS             50444..50788
                     /locus_tag="SCJ86_000159"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289451.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosomal-processing cysteine protease Prp"
                     /protein_id="HEG1167754.1"
                     /translation="MIKGTFKRNDAGQIVSFTLTGHADAGPYGSDIVCAGVSALAIST
                     VNGIASLAGFEPIVEMNEEEGGYLYTEVTSGMTQEQNNIAEILLENLLLGLQSIEAEN
                     SEYIQIKTINDK"
     gene            50801..51094
                     /gene="rpmA"
                     /locus_tag="SCJ86_000160"
     CDS             50801..51094
                     /gene="rpmA"
                     /locus_tag="SCJ86_000160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289452.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L27"
                     /protein_id="HEG1167755.1"
                     /translation="MLLSMNLQLFAHKKGGGSTSNGRDSESKRLGAKRADGQTVTGGS
                     ILYRQRGTKIYPGVNVGIGGDDTLFAKVDGVVRFERKGRNKKQVSVYPVAQEA"
     gene            51528..54335
                     /locus_tag="SCJ86_000161"
     CDS             51528..54335
                     /locus_tag="SCJ86_000161"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629471.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma 54-interacting transcriptional regulator"
                     /protein_id="HEG1167756.1"
                     /translation="MYVKEKTAHLTKAEYDQGVTTQEIAEVLGIQRTNSSKDLNQLVR
                     EGKLIKTDGRPVRYIYQTLVTHVKPVTKHVVSYKETPVSEEKNTLQIDTKDIFAKIIG
                     ANGSMKNSVEQAKAAILYPPRGLNCLITGPTGSGKTYFAHAMYHFAKAHHVIDEETEL
                     IVFNCADYANNPELLMSHLFGYAKGAFTGADEEKMGIIDQADGGMLFLDEIHRLPPEG
                     QEMIFYFMDHGTYSRLGETTKSHEANVRIVGATTEDPGSSLLETFVRRIPINIKLPAF
                     EKRPANEKVDLVKIMIAHEANRTQRKISLTEDVAKALIGSVTYGNIGQLKSNIQLVCA
                     RGFLNHMQSPEISITIDDLTEGIRSGLIQLASNRSAMAELSKILEPKITVAPNDTIMK
                     IQSDSYELPYNLYDIIGDKAALLKSDGLDQEAINHFISTDINIHLKSFYKDHGFSFNA
                     DSKLAEFVDPKIIEVTNQIYVMVKRSLPYEFQQNFIYAMSLHISSFLKKINLGEERHT
                     NDNIREMAFDFPDEYAVAKEVRSYIETYFQVEIPESEDYYLTVLLVSLRANQASGRIG
                     VVVAAHGNSTASSMVQVVQQLLDAENVRAVDMPLDMDPKTALDRIERNVQEVDEGSGV
                     ILLVDMGSLASFNSQIQRDTGIPVRTVDMVTTSLVLETVRKVSVLGTDLDMLHESLKN
                     FRGYAEISSVESVQEAKPIHKKKAVLAICASGEGTAQRIKELIERAVNKRQETELEVL
                     ALSIVEVKEELPVIQKEYQIIATTGITDLNIQAPFIPLERFIDQNIEVILDQLLMESE
                     LEETEFISLDEESAKNTCVEFISDNFIFINGSKLIDPMWQFSTQISQTTGIGDEEYGF
                     KINLVMHTAGMIERIIRNEPLTVEENELTNTTNDPLYSQLAASVVLLEDQIKVKVPIE
                     EMYYLLRLVHNQLDKKEYTVP"
     gene            54526..55017
                     /locus_tag="SCJ86_000162"
     CDS             54526..55017
                     /locus_tag="SCJ86_000162"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342244.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HEG1167757.1"
                     /translation="MDIRLARIDDRLIHGQVATVWSKQTGVERIIVISDVVAQDKLRK
                     FLLKEAAPPGITANVITVQKMIQIYHSGLLSRAKVMLLFTNPQDVEAVVRCGVQLKSL
                     NIGGMSFTEGKSMITNFVSVNQQDIDSFRYLNSQEVELEIRKVPADRKILLMELLAKI
                     KKV"
     gene            55120..56109
                     /gene="manX"
                     /locus_tag="SCJ86_000163"
     CDS             55120..56109
                     /gene="manX"
                     /locus_tag="SCJ86_000163"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289456.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS mannose transporter subunit IIAB"
                     /protein_id="HEG1167758.1"
                     /translation="MVGIILASHGQFAEGILQSGSMIFGEQENVKAVILKPSEGPDDL
                     RAKLEEAVASFDNQDEVLFLVDLWGGTPFNQSNTLFEEHKDKWAIVSGLNLPMLIEAY
                     ASRFSMESAHEIAAHIIETAKDGVKVKPEELEPAEAPKAAVEDAQPKGALPEGTVVGD
                     GKIKYVLARVDSRLLHGQVATAWTKAVQPNRIIVVSDAVSKDDLRKRLIEQAAPPGVK
                     ANVIPISKMIEVAKDPRFGNTKALLLFENPEDVLTAVEGGVDIKELNVGSMAHSVGKV
                     VVSKVLSMGQEDVEAFEKLEQKGVKFDVRKVPNDSRDNMDDILKKAKAELAKA"
     gene            56142..56945
                     /locus_tag="SCJ86_000164"
     CDS             56142..56945
                     /locus_tag="SCJ86_000164"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010719801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS mannose/fructose/sorbose transporter subunit
                     IIC"
                     /protein_id="HEG1167759.1"
                     /translation="MSIISIILVVLVAFLAGMEGILDEFQFHQPLVACTLIGLVTGNL
                     EAGIVLGGTLQMIALGWANIGAAVAPDAALASVASAIILVLGGQGVKGVPSAIAIAVP
                     LAVAGLFLTMIVRTIAVPIVHLMDAAAEEGNIRKVEMWHIIAVCLQGVRIAVPAAALL
                     FIPAQTVQSFLESMPAWLTDGMAIGGGMVVAVGYALVINMMATKEVWPFFVIGFVVAA
                     ISQLTLIALGALGVALALIYLNLSKMGGSSNNGGGSNSGDPLGDILNDY"
     gene            56966..57880
                     /locus_tag="SCJ86_000165"
     CDS             56966..57880
                     /locus_tag="SCJ86_000165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010751508.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS mannose/fructose/sorbose transporter family
                     subunit IID"
                     /protein_id="HEG1167760.1"
                     /translation="MAEEKIKLSKKDRLAVAWRSTFIQGSWNYERMQNGGWAFSMIPA
                     IKKLYKTKEERSAALKRHLEFFNTHPYIASPILGVTLALEEERANGAPVDDVAIQGVK
                     VGMMGPLAGVGDPVFWFTVRPMLGALGASLAMGGNILGPIIFFLGWNIIRWAFMWYTQ
                     EFGYKAGSKITDDLSGGLLQDVTKGASILGMFVLAALVQRWVSIKFLPVVSTVKLDKG
                     AYIEWDKLPSGGEGIKSAFEQVNNGLALSPEKVTTLQDNLDQLIPGLAALGLTLLCMW
                     LLKKKVSPIVIILGLFVVGVVGHVIGLL"
     gene            57963..58337
                     /locus_tag="SCJ86_000166"
     CDS             57963..58337
                     /locus_tag="SCJ86_000166"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF956 family protein"
                     /protein_id="HEG1167761.1"
                     /translation="MVQSINTKVDLVIDATSFTGLTDYGQIMIGDNGFEFYHARDRRK
                     YIQIPWEEVDYVLASVMLKGKWIPRYAIQTKKNGTYTFSSKEPKKVLRAIREYVDEDR
                     MVQSLSFFDVVKRGIRSMFKRK"
     gene            58476..58721
                     /locus_tag="SCJ86_000167"
     CDS             58476..58721
                     /locus_tag="SCJ86_000167"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736004.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GlsB/YeaQ/YmgE family stress response membrane
                     protein"
                     /protein_id="HEG1167762.1"
                     /translation="MSWLWTLIVGAVIGAIAGAITSKGNSMGCVTNIIAGLVGSSIGQ
                     AIFGEWGPKAAGMALIPSILGAVILVAVVSFFFGKRS"
     gene            58893..59957
                     /locus_tag="SCJ86_000168"
     CDS             58893..59957
                     /locus_tag="SCJ86_000168"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase P family protein"
                     /protein_id="HEG1167763.1"
                     /translation="MMLRVEKLRKKMQEENLDSFLVTSPYNLRYLTNFTGTTGLAVIT
                     LEKAFFITDFRYTEQAAAQAQGFEIIKNVGPIFDEVANLVRKEELHSLAFEETTVSFL
                     EYSVLEEIIDAELVPVSQMIEELREVKDEEEIAIIEKACHIADMAYDHILKMIQPGMT
                     EIEVANQLDFYMRSLGASGVSFDTIVASGLRSAMPHGVASKKIIEQGDLITLDFGCYY
                     EGYVSDMTRTFAIGDPGEKLKEIYQIVLDAQLAVIDAAKPGMTGVQLDAVARDYISKH
                     GYGEAFGHSTGHGIGLEIHEGPNVSSRAEKQFVPGNVITDEPGIYLPGIGGVRIEDDL
                     LITEDGNRVLTRSPKELIIL"
     gene            60203..60628
                     /locus_tag="SCJ86_000169"
     CDS             60203..60628
                     /locus_tag="SCJ86_000169"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016624160.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Asp23/Gls24 family envelope stress response
                     protein"
                     /protein_id="HEG1167764.1"
                     /translation="MADEKNLILNANQDLGEIVIAPEVIEVIIGIAASKVEGVYGMRG
                     TFASNVTELLGRAAHGKGVYLANEEDGLKVDLYCYLEYGVSVPKVAMEMQERVSQQVL
                     FMTDIDLSEVNIHVVAVVPEKLEQPTIEELFEDEEEENE"
     gene            60621..61070
                     /gene="nusB"
                     /locus_tag="SCJ86_000170"
     CDS             60621..61070
                     /gene="nusB"
                     /locus_tag="SCJ86_000170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010751503.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription antitermination factor NusB"
                     /protein_id="HEG1167765.1"
                     /translation="MNKELSRHEIREMALQALFPLDFNADLTKEDAIFNAIELDHRDM
                     INEDESEFVPVYLDTLVGGVCAKKDELDKVIEKHLKNNWHINRISKMDLVILRIAIFE
                     MTYVTDVPAAVALNEAIELAKTFSDDRSRKFVNGVLSNVLKELEAGA"
     gene            61167..62021
                     /locus_tag="SCJ86_000171"
     CDS             61167..62021
                     /locus_tag="SCJ86_000171"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345576.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional methylenetetrahydrofolate
                     dehydrogenase/methenyltetrahydrofolate cyclohydrolase"
                     /protein_id="HEG1167766.1"
                     /translation="MAELINGKELAEKMQAEIAEKIQKLKDNGIHPGLVVLLVGENPA
                     SQIYVRNKERVAKEIGIHSLVERYPETISEDELLAEIEKYNQDPSFHGILVQLPLPKH
                     IDEEKILLAIRPEKDVDGFHPMNLGRLLAGKPDKIPCTPYGIMKMFEAYSIDPAGKRA
                     VVIGRSNIVGKPMAQLLLMADATVTVAHSKTENLAELAQEADILVVAIGRGHFVTKEF
                     VKPGAVVIDVGMNRDANGKLIGDVKFDEVEPIAGWITPVPKGVGPMTITMLMYQTVKS
                     AEAFGAGE"
     gene            62022..63359
                     /locus_tag="SCJ86_000172"
     CDS             62022..63359
                     /locus_tag="SCJ86_000172"
                     /EC_number="3.1.11.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344045.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease VII large subunit"
                     /protein_id="HEG1167767.1"
                     /translation="MAQEYLTVTTLTKYLKRKFDADPYLERVYLTGEISNFRLRANAH
                     QYFSLKDDHAKISAIMFKGAFQKLRFQPKEGMKVLVVGRISLYEAGGSYQIYVEHMEP
                     DGIGALYQELEERKEKLMKEGLFQGPKKMLPRFPKRIAVLTSPSGAVIRDIITTVKRR
                     YPIVQLVLFPTVVQGNQAADNVVRNIQRVEEMGNFDTMIIGRGGGSIEDLWPFNEERV
                     ARAIYQANTPVISSVGHETDTTIADLVADVRAATPTAAAELAVPVLSEELMRIQERQA
                     RLQQAFVRQIQRKQERFERARRSYVFRQPERLYEGQTIKLDQLKQRLYQATQQIYHAK
                     EKQTIALSHQLEQVAPLYRVKAARQETAYLEKRLEEKIQQYMKQQQQRFAQAVQSLDL
                     LSPLKIMGRGYSYTTLNDQVVKSVTEIHQGDLLTIHYQDGIVKGQVQEIDKGE"
     gene            63364..63588
                     /locus_tag="SCJ86_000173"
     CDS             63364..63588
                     /locus_tag="SCJ86_000173"
                     /EC_number="3.1.11.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016624156.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease VII small subunit"
                     /protein_id="HEG1167768.1"
                     /translation="MENPTFEESLQELEKIVMQLEQGDVPLESALDSFKRGMELSKHC
                     QDTLAKAEETLTKMMTESNEEVDFDGNEES"
     gene            63590..64480
                     /locus_tag="SCJ86_000174"
     CDS             63590..64480
                     /locus_tag="SCJ86_000174"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628680.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polyprenyl synthetase family protein"
                     /protein_id="HEG1167769.1"
                     /translation="MRLTDFSAIHLPAVEKEILSFLDEHTTDRGLYDAMSYSVKAGGK
                     RIRPLLLLTAVASFDEPIDVPVYQVAAALEMVHTYSLIHDDLPAMDNDDLRRGKPTNH
                     KVFGEALAILAGDGLLTGAFQLISMAHLGNSPKLLLLQQLAVCAGSQGMVAGQAADIE
                     GESKKLSLEELAFIHERKTGHLIRYALLAGGILAKQPEEILLLLQRLAEHLGLAFQIR
                     DDLLDVIGTTKTLGKTAGKDERMEKNTYPRLLGLEKTREALEIELLSANKIIDKLEED
                     VFSFDGGLMRQMIKQFDVEK"
     gene            64489..65307
                     /locus_tag="SCJ86_000175"
     CDS             64489..65307
                     /locus_tag="SCJ86_000175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326037.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TlyA family RNA methyltransferase"
                     /protein_id="HEG1167770.1"
                     /translation="MEKERVDVLAVKQGLFETREQAKRSVMAGLIYNEKNERFDKPGE
                     KIPVSSELRVKGKKLPYVSRGGLKLEKALKAFDLSVEGKTLLDIGASTGGFTDAALQN
                     GAKMSYALDVGYNQLAWKIRQDPRVVVMERVNFRYAKPEDFTLGIPEVAVIDVSFISL
                     KLMLPPLHAILKEEGEVIALIKPQFEAGREAVGKNGIVRDPQTHQKVLEDILSFAAQG
                     GYDVLELSYSPITGGEGNIEFLAHLRKVPESGTINSAINMAEVVSNAHEQFDHK"
     gene            65414..65863
                     /locus_tag="SCJ86_000176"
     CDS             65414..65863
                     /locus_tag="SCJ86_000176"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291879.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginine repressor"
                     /protein_id="HEG1167771.1"
                     /translation="MRKKDRHRLITRLLSEQDIRKQEEFVEILKSKGISVTQATISRD
                     IKELKLIKVPASDGGYRYSIPAETNEDVNTKLEKLLKDAFVAVDQMEKFVILKTLPGN
                     ASAAANLIDKRYKKELFSIINDDDNVLMITRTEDQAVLLKKDFLHYL"
     gene            65881..67563
                     /gene="recN"
                     /locus_tag="SCJ86_000177"
     CDS             65881..67563
                     /gene="recN"
                     /locus_tag="SCJ86_000177"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628044.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RecN"
                     /protein_id="HEG1167772.1"
                     /translation="MLQEISITNFAIIPELRLSFHEGMTALTGETGAGKSIIIDALGL
                     LAGGRGSSDYIRQGAEKCVLEGLFELPKQEGFSELMVELGIETDEDNLIVRRDMSLTG
                     KNVCRVNGHIITLANLRRIGSYLVDIQGQNEHQELLQPESHLALLDRFGDVAFQQKKK
                     SYQQEYISYRELEKRVRKVQQNEKSYVQRIDMLHFQQEEIAAADLQVGEEEKLKEERE
                     KLSNYQKIADGLAAGYGALTNNEQNSVDGVGLAVSELQGIAHLDVEYEAIYENIQSAY
                     YLLQDAIGDMSRQIDLLELDESRLEEVTQRLELIRQLKRKYGESIESILAYYDEITEE
                     LASSDFSEGQLDKMKEELEQKELLLQQQAADLHEARKKIAKELEKSILHELKSLYMEN
                     TEFEVRFLKEENRQLNHDGFDQIEFYITTNPGEPLKPLVKVASGGELSRMLLALKTIF
                     SSEQGVTSIIFDEVDTGVSGRVAQAIADKISQISKYSQVLCITHLPQVAAVADYQYYI
                     VKAVIDGRTQTSVSELKTKEREEEIARMLAGSEITKLTLEHAKELLKLAKRT"
     gene            complement(67618..67734)
                     /locus_tag="SCJ86_000178"
     CDS             complement(67618..67734)
                     /locus_tag="SCJ86_000178"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291876.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4044 domain-containing protein"
                     /protein_id="HEG1167773.1"
                     /translation="MREKKQTSTFAKVTKFVIWTMLILTIGSVVLTAVMSVM"
     gene            complement(67807..68763)
                     /locus_tag="SCJ86_000179"
     CDS             complement(67807..68763)
                     /locus_tag="SCJ86_000179"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346774.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium transporter CorA family protein"
                     /protein_id="HEG1167774.1"
                     /translation="MIKYFTLDNEKLRNTGKETDQTIWYCVERPNQEEIHQLTHQFEI
                     PSDYITSILDDAENSRTEEFNQSKLTKPALLLLQYPFAKTSPSGYFQVDTYPLSIILT
                     PKKKLITITNHEPLFLKKVFSQTFPKSDISPNLNIFFQFIWQVTLSYNSYLATLQSQC
                     DTLEAKLQVSTENKQLYQIMDIQKSLVYFREATSANLDALKELAQNKLFQENHALKNH
                     LRDVLVETEQAMTTSRIQLKLVEQMNQTFSAIVSNNLNNIMKILTSLTIILTIPTIVG
                     SIYGMNIKLPIAERDDAFIWLVLLTLFICIVTTYYLKKGKFL"
     gene            68934..69302
                     /locus_tag="SCJ86_000180"
     CDS             68934..69302
                     /locus_tag="SCJ86_000180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327166.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3397 domain-containing protein"
                     /protein_id="HEG1167775.1"
                     /translation="MGTFTPLILFWYIYPVIVLFGCQFLVKLFSLNRRFKIKAPDLAI
                     PFLWGGMHALSRNTGTISILPFFFISVLLMGICIAVFQAYYYEEIIYPRYFKMTWRLV
                     FLLTIVLYAVLIIVNILSYL"
     gene            69472..69903
                     /gene="mraZ"
                     /locus_tag="SCJ86_000181"
     CDS             69472..69903
                     /gene="mraZ"
                     /locus_tag="SCJ86_000181"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005881057.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="division/cell wall cluster transcriptional
                     repressor MraZ"
                     /protein_id="HEG1167776.1"
                     /translation="MLMGEFQHNIDAKGRLIVPSKLREELGEKFVLTRGLDGCLFGYP
                     MSEWENLEAKLNEMPLAKKDARTFVRFFYSAATECELDKQGRINIPSTLRNYAALTKG
                     CVVIGVSNRIEIWDETRWQDFSAAAEENFDEIAESMIDFGL"
     gene            69924..70883
                     /gene="rsmH"
                     /locus_tag="SCJ86_000182"
     CDS             69924..70883
                     /gene="rsmH"
                     /locus_tag="SCJ86_000182"
                     /EC_number="2.1.1.199"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348714.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (cytosine(1402)-N(4))-methyltransferase
                     RsmH"
                     /protein_id="HEG1167777.1"
                     /translation="MTETFQHYTVMLKETVDGLNIKPDGVYVDCTLGGAGHSEYLLSQ
                     LSSKGHLYAFDQDQKAIDHAKLRLASYVEQGMVTFIKANFRELEERLSEHVDKVDGIL
                     YDLGVSSPQLDEAERGFSYHQDAPLDMRMDQSAPLSAYHVINEYSYHELVKIFFRYGE
                     EKFSKQIAREIERVRKTKPIQTTGELVEIIKQVIPAPARRKGGHPAKRIFQAVRIAVN
                     DELGAEEASLEQAIRLLKVNGRISVITFHSLEDRIVKSMFKEYSTVQDLPPGLPVIPD
                     EFQPELKVITRKPILPSEEELTENNRSRSAKLRIAEKIRMKEE"
     gene            70888..71289
                     /gene="ftsL"
                     /locus_tag="SCJ86_000183"
     CDS             70888..71289
                     /gene="ftsL"
                     /locus_tag="SCJ86_000183"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291866.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsL"
                     /protein_id="HEG1167778.1"
                     /translation="MAELKKMQDYEYDLPELVDQPEQQMPDSSSNQEIILPQSPAKRL
                     KQISALEKIAVASVAIAIVALCVLTVMLRTNISGAEKEITSIQTEINDKKQEKTSLEQ
                     EKNELSRTERIKQIAEEKGLSINDDNLRKVK"
     gene            71290..73482
                     /locus_tag="SCJ86_000184"
     CDS             71290..73482
                     /locus_tag="SCJ86_000184"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326487.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding protein"
                     /protein_id="HEG1167779.1"
                     /translation="MSLKNKIRRFMEKKNLNPMNNRKKVGIILFATSIGLFFLFAVRF
                     SYIVIGGHVAGTSLAEKTKQLYQGSEVVKAKRGTIYDRNGVALAEDASSYSIKAILSK
                     TYTSGDKKLYVEEKNFDKIAEILHKNLSIDKKDALNILEDGAKKELYQVEFGSYGKNI
                     SQETKQNIEADMKKEGVAGLYFVDHQARMYPNGVFSSHFIGYAVPDKDENGLVGKLGL
                     ESAYNDILSGKDGKIIYQKDNFQNPLPGTVAEEEKAVDGQDIYTTLDSRLQSYLETLM
                     DQVNEEYQPEELTAVLMKAKTGEILAMGQRPTFNPETMEGLTGKDAIWRNFLVQDSYE
                     PGSTMKVFTTAAAIEEGEFNENETFQSGKIQVEDATINDHDFGEKGVLTMRQALSWSS
                     NVGMVILEQRLGGRWYNYLQKLGFGQSTHSGLDDEVNGALPTLNIVDRAMSAYGQAVG
                     VTNFQMMKAFTSIANNGTMIQPRYISKVVDPQTGEERTTQTEVLGQPFSKETTEKVRE
                     YMRDVVESENYGSAYGVYSVPGYNVSAKNGTAQIASDTGGYQTGDTAYLYSIVEMVPS
                     EDPDYVLYLTMKHPKTYDRMALAKIANPLMKRAMDFKETEEDSDTETKTEKVSVADYR
                     NLEADVAAADAQKSGLQPVVIGNGKKVQKQSTANGDQLISGEKLILYTGGDKLMPDVT
                     GWSKADIMKLGKILGIEVSFDGDGYCVKQELAPYEKITEDKLSFTLEE"
     gene            73508..74470
                     /locus_tag="SCJ86_000185"
     CDS             73508..74470
                     /locus_tag="SCJ86_000185"
                     /EC_number="2.7.8.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phospho-N-acetylmuramoyl-pentapeptide-
                     transferase"
                     /protein_id="HEG1167780.1"
                     /translation="MEWTQALIPIVSSCAMTIAAMPLFIGYFQMKKQGQAIREEGPKW
                     HNSKAGTPTMGGLVFLIGSILTGIWVGAWQKQLTPTLFILLFVLALYGVIGFLDDFIK
                     IFKKRNMGLNSKQKLIGQILGGIIFYLVYRSEGYPGVLNFFGLDLPLGIIYGIFALFW
                     LVGFSNAVNLTDGIDGLVAGLGSISFAAYGLIAWHQHQYDVLIICLSVLGGLLGFFVY
                     NRKPAKIFMGDVGSLALGGLLAAISIMLNQEWTLLLIGLIYVMETASVMLQVTSFKLT
                     GKRIFKMSPIHHHFEMCGWSEWKIDTVFWLISIVTSLITLWIVW"
     gene            74484..75845
                     /locus_tag="SCJ86_000186"
     CDS             74484..75845
                     /locus_tag="SCJ86_000186"
                     /EC_number="6.3.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002391744.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-L-alanine--D-glutamate
                     ligase"
                     /protein_id="HEG1167781.1"
                     /translation="MKKISTYENKKVLVLGLAKSGVSAAKLLHELGALVTVNDGKPFD
                     ENPEAQELLSLGIKVITGSHPIELLDEEFSLMVKNPGIPYSHPLVAKAQEMGIPVITE
                     VELAYEVAECPIIGITGTNGKTTTTTMTGLLLNAGADQGIARLAGNIGYPASGVAQEA
                     KSEDKIVMELSSFQLMGITDFRPHIAVITNIYEAHIDYHGTRKEYVKAKWNLQKNMTE
                     KDYLILNWNQSELQELAQRTKARVLPFSTKEVLEDGVYADDYSIYYKKEKIMEISELG
                     VPGKHNVENALAAISVAKLYGISNEAIRETLHFFHGVPHRTQYVGEIQGRKFYNDSKA
                     TNILATKMALSGFETSKVVLLAGGLDRGNTFDELIPSLKGIKAMVVFGQTKEKLMDAG
                     KKAGIETIVTADSVEQAVPLALENSTDGDVVLLSPANASWDQYPNFETRGNRFMEAVN
                     RLK"
     gene            75871..76959
                     /gene="murG"
                     /locus_tag="SCJ86_000187"
     CDS             75871..76959
                     /gene="murG"
                     /locus_tag="SCJ86_000187"
                     /EC_number="2.4.1.227"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346310.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="undecaprenyldiphospho-muramoylpentapeptide
                     beta-N-acetylglucosaminyltransferase"
                     /protein_id="HEG1167782.1"
                     /translation="MKILVTGGGTGGHIYPALAFVNYVKTQEPNAEFMYVGAKRGLEN
                     KIVPDTGMPFHTLEIQGFKRKISMHNIKTVRLFLKSIRQAKKILSDFQPDIVIGTGGY
                     VSGAVVYAAAKMGIPTVIHEQNSVPGVTNKFLSRYVDRIALSFKDAASFFPENKAVLV
                     GNPRAQEVADTKKSDILKTFDLDPEKKTVLIFGGSQGALKINQAVSVFLSEYIPSSYQ
                     VLYASGDRYYEEILNKINPEIQNISVQPYIKNMAEVMASSDLLVGRAGATSIAEFTAL
                     GLPAVLVPSPYVTNDHQTKNAMSLVHAGAAKMIADNELTGESLSQTVNEIMGDEELQK
                     QMCRASKEQGIPDASKRLYDLVKQIIKK"
     gene            76974..78194
                     /locus_tag="SCJ86_000188"
     CDS             76974..78194
                     /locus_tag="SCJ86_000188"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377092.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsQ/DivIB"
                     /protein_id="HEG1167783.1"
                     /translation="MSKKKEPKKLSHPSSDENNLTPWQKANRKYLAEQEMKNQEPKKE
                     DITLDTPLSDSEEEKTVQEEQQEESQSEKKIEFFEEIKQKKNGGPYNGSFLNRLPNLK
                     SQRHKVLFRRLALIITVLGIPLIFLIYYVSPYSKLQAVAVSGNKTVNSQEIISDTKLS
                     LGENVWGQYFHRSTYIDRLKKAQPRIETANIHFKGMNEFDLDVTEYKEIALIAKNNQY
                     YPVIENGTVLDEKVANPTKNLPILEEFKDSAKIKELTKQYNQLSSELQKAISEIKYTP
                     RASNKNLIQLNMNDGNQVIVNINNLANQMKYYSQVAKDMDEKGVIDMEVGIFSHPYGS
                     TQETEESGQSEETTESEENADQTNESSEEAETQKAQTPGSSSTNGSSDSNDSSETSQI
                     EDNPSTTPSSLNEN"
     gene            78350..79675
                     /gene="ftsA"
                     /locus_tag="SCJ86_000189"
     CDS             78350..79675
                     /gene="ftsA"
                     /locus_tag="SCJ86_000189"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347904.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsA"
                     /protein_id="HEG1167784.1"
                     /translation="MAKTGMYVGLDIGTTSVKVVVAEYIDSQMNIIGVGNAKSEGINR
                     GIIVDIDKTVQAIQRAVRQAEEKAGIQIKGVSVGLPANMLEVENCQGMIAVNGDSKEI
                     TDEDVRNVASAALVRSIPPERQIVSILPQDFTVDGFEGIKDPRGMIGVRLEMYGLLFT
                     GPKTIIHNIRKCVENAGLIVNEMVITPLALTESILSDGEKDFGTIVIDMGGGQTTTAV
                     MHDKQLKFTNLDQEGGEFVTKDISIVLNTSFNNAEALKINYGDAYPERTSPDEEFPVD
                     VIGQSEPVKVDERYLSEIISARMEQIFNKAKDALDQIEALELPGGVVLTGGAASLPGV
                     VDLAQEIFGVNVKLYVPNHMGLRNPVFTNVISIVDYSANLSEVYQLAKIAVTGETSAA
                     RQMVVEQETTNTYESYEAPEETIYDEPEPKESGENVKNKIKGFFTNIFD"
     gene            79698..80939
                     /gene="ftsZ"
                     /locus_tag="SCJ86_000190"
     CDS             79698..80939
                     /gene="ftsZ"
                     /locus_tag="SCJ86_000190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346312.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsZ"
                     /protein_id="HEG1167785.1"
                     /translation="MEFSIDNNINDGAVIKVIGVGGGGGNAVNRMIEENVKGVEFITA
                     NTDVQALKNSKAETVIQLGPKYTRGLGAGSQPEVGQKAAEESEQSLREALDGADMIFI
                     TAGMGGGTGTGAAPIVAGIAKELGALTVGVVTRPFTFEGPKRGRFAAEGIARLKENVD
                     TLLIISNNRLLEVVDKKTPMLEAFREADNVLRQGVQGISDLITAPGYVNLDFADVKTV
                     MENQGTALMGIGVASGEDRVVEATKKAISSPLLETSIDGAEQVLLNITGGLDMTLFEA
                     QDASDIVANAATGDVNIILGTSINEEMGDEIRVTVIATGIDESKKERKSSRPARQAQM
                     QSPAQKTVLDMDQAKPTSSEEENSFGDWDIRREQNVRPRVDDSNFDNIEKKEFDTFNR
                     EETKSHGDDELNTPPFFRRKK"
     gene            80956..81633
                     /locus_tag="SCJ86_000191"
     CDS             80956..81633
                     /locus_tag="SCJ86_000191"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344053.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YggS family pyridoxal phosphate-dependent
                     enzyme"
                     /protein_id="HEG1167786.1"
                     /translation="MIADNLQKIKQEIQDSCALVHRDPKEVTLVAVTKSVESAQAEEL
                     VRAGVMNCAENRVDKLLEKKQDLFDFPEIKWHLIGNLQRRKVKLIVNEIDYFHALDSL
                     RLAEEIQKRLEKELSCFIEVNVSGEESKHGISPDELLPFVESLAACTKIKVVGLMTMA
                     PKEASDEQIHKVFGTLKELRDLVQAKGYAHAPCSELSMGMSQDFQIAIEEGATFIRVG
                     TALFKDE"
     gene            81647..82246
                     /locus_tag="SCJ86_000192"
     CDS             81647..82246
                     /locus_tag="SCJ86_000192"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326044.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein SepF"
                     /protein_id="HEG1167787.1"
                     /translation="MPLLNRGAIANFFGLADEEEYEYQDYPETTKKAVQPTKKNTTQN
                     QSTHSVSAANTHEKQKTATSRPVTHESIQPAEKKKVIELHQSPTKTVAKERTASRTST
                     QPKANLAGKITVMEPRAYSEAMAIAKRIIAGEATLVNFHLVEEKQARRIVDFLTGTVY
                     ALDGDIKRVGDEIFLCTPTGMEIDSSTAQSFADANLFDL"
     gene            82261..82548
                     /locus_tag="SCJ86_000193"
     CDS             82261..82548
                     /locus_tag="SCJ86_000193"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007207099.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YggT family protein"
                     /protein_id="HEG1167788.1"
                     /translation="MYTIALLLHLLQRAAYFYSIILMVYALLSWFPGGYQSGLGRLLA
                     KICEPYISLFDRLPLRIGPLDFTILGAILILNLAVQGLAQIVIWITVHLVY"
     gene            82597..83379
                     /locus_tag="SCJ86_000194"
     CDS             82597..83379
                     /locus_tag="SCJ86_000194"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177078.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-binding protein"
                     /protein_id="HEG1167789.1"
                     /translation="MDANVYQHFRADERTFIDSVGDWIEQVQGQYAPYLTEFLDPRQS
                     YILETLIRQSSDLRFMFYGGYEQAERKRCLIFPDYYEPVEEDFETALYEIHYPSKFAS
                     LSHGKILGTLVGTGIKRSYFGDIISDGDRWQVFIANEVDHFVAAQVTKIGKVAVRLEP
                     IKYTEMIMPKDSWTQERGTVSSMRLDSVISEIYNISRQRSKQLIEAGKVKVNWTENTR
                     PDFVLELLDIISVRGYGRVQIQELEGKTKKEKYRVLFGVLRK"
     gene            83400..84107
                     /locus_tag="SCJ86_000195"
     CDS             83400..84107
                     /locus_tag="SCJ86_000195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629467.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DivIVA domain-containing protein"
                     /protein_id="HEG1167790.1"
                     /translation="MALTPLDIQNKTFPTKMRGYNQDEVDDFLDLVVRDYEELTQRNR
                     ELEKAVKHSEEKLEYFNELKDALNQSIIVAQDTADKVKTSASKESEVIVTSAQNKADE
                     LVANAEKRAHQLTTDAEEKARKILTDATEKARQLATETEDLKKKTRVFHQRISLMLES
                     QLEQVKSPEWDEILQPFSSYVTDSHEVIKEVLAKQLDNENDTDVNSDTGVIEAPMTAF
                     DTQAIDISIVPGENENE"
     gene            84369..87167
                     /gene="ileS"
                     /locus_tag="SCJ86_000196"
     CDS             84369..87167
                     /gene="ileS"
                     /locus_tag="SCJ86_000196"
                     /EC_number="6.1.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628049.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="isoleucine--tRNA ligase"
                     /protein_id="HEG1167791.1"
                     /translation="MKMKETLQLGKTKFPMRGNLPNREGEWQKEWEENRIYQKRQELN
                     EGKPSFVLHDGPPYANGNIHLGHSLNKISKDIIIRSKSMSGYRAPYVPGWDTHGLPIE
                     QVLANKGVKRKEMSMAEYLEKCREYALSQVDKQRADFKRLGVAGDWEHPYVTLDPSYE
                     AAQVRVFGKMAEKGYIYKGLKPIYWSPSSESSLAEAEIEYKDVKSPSIFVAFNVKDGK
                     DILDEDTAFVIWTTTPWTLPANQGIAVNPKFTYVLVEADGRKFVVAKDLLETVQSEIG
                     WENVTVLKEFAGQEMEYMTASHPFYERESLVILGDHVTLDAGTGLVHTAPGHGEDDYI
                     AGSKYKLSVVSPVDSKGLFTEEAPGFEGIFYDKANPMITDLLKEKGALLKLDFFTHSY
                     PHDWRTKKPVIYRATPQWFASIDKFRQNILDEVEKVEWVIPWGKTRLYNMIRDRGDWV
                     ISRQRAWGVPLPIFYAENGEAIITPETIEHVAKLFAEHGSIIWFEKEAKELLPEGFTH
                     PGSPNGEFTKEKDIMDVWFDSGSSHEAVLRQRPELSFPADMYLEGSDQYRGWFNSSIT
                     TSVAINGVAPYKSVISQGFALDGEGRKMSKSLGNIIAPEKVIKQFGADILRLWVSSVD
                     YEADVRVSMDILSQVAEVYRKIRNTMRFLLANTEDFDPKKDTVSYNDLRSVDKYMTVR
                     LNQVIKEIREEGYDKYNFMHIYRTVMNFLTVDLSSFYLDFAKDVVYIEAEDDYQRRCM
                     QTVFYQTAVALTKLLTPIIPHTAEEIWSFLKEEEEYVQLSEFPGYEEFANQEELMDTW
                     TAFMDFRDNVLKALEEARNSKLIGKSLEAKLTVYPKQQVREMLKALDADIAQLLIVSP
                     DYFEVMAADEQVPDNAMVFDDVAITVEKADGETCDRCRQVRKDVGVDEKLPHLCGRCA
                     KIVEDFYPEAVAEGFEEK"
     gene            complement(87216..88742)
                     /locus_tag="SCJ86_000197"
     CDS             complement(87216..88742)
                     /locus_tag="SCJ86_000197"
                     /EC_number="1.1.1.49"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286618.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-6-phosphate dehydrogenase"
                     /protein_id="HEG1167792.1"
                     /translation="MSNEKNVLFTIFGGTGDLAQRKLYPSLFRLYRKGNLGEHFAVIG
                     TARRPWSDEHYREVVKETIRALNPSDEEATTFASHFYYQSHDVNDSTHYQTLKELSDR
                     LDGHYQLNGNRVYYLAMAPQFFGTIVSHLKSQNIMTKNGFNRLIIEKPFGSDYQSAFE
                     LNQQIREVFPEQDIFRIDHYLGKEMIQNISAIRFANNIFEAQWNNRYIDNIQITFGEN
                     LGVEDRGGYYDHSGALKDMVQNHILQVVALLAMEPPVAFSEKEIRTEKIKALKAIRIY
                     NEEEVLENFVRGQYAQGELDGVQFKGYREEDKVDAQSETETFVAGKFTIDNFRWSGVP
                     FYVRTGKRLTEKGTRINIVFKQVPVNVFKTSVDEPCDDTTLPPNVLTIYIQPTEGFSL
                     SLNGKEVGQGFETEPIKLEFRNSAEMVENSPEAYEKLLLDALNGDGTNFSHWEEVAQS
                     WHIVDVIRKAWDQTQPDFPNYKAGSMGPKAAFELLEKDGFEWIWQPDNWYRERGKLND
                     "
     gene            complement(88757..89404)
                     /locus_tag="SCJ86_000198"
     CDS             complement(88757..89404)
                     /locus_tag="SCJ86_000198"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310285.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal-dependent transcriptional regulator"
                     /protein_id="HEG1167793.1"
                     /translation="MTPNREDYLKIILELGGDTTKVNNKQIVSSLDVSAASVSEMISK
                     LVKEELVEHSPYQGVQLTASGLQKASSLIRKHRLWEVFLVEHLDYSWNEVHDDAEVLE
                     HVTSEHLADHLENYLNHPEHCPHGGSIPKKEEVVHEERRQTLESYPVGTKVRIARVLD
                     EKELLDYLVTIDLNINEEYEIVDIAAYEGPITIQNDRKKIPVSFKAASTIFVDKI"
     gene            complement(89588..89917)
                     /locus_tag="SCJ86_000199"
     CDS             complement(89588..89917)
                     /locus_tag="SCJ86_000199"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286678.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4828 domain-containing protein"
                     /protein_id="HEG1167794.1"
                     /translation="MIYFLGSLITVLSSFIYLKKKKQAEKSEGLSKYQGTWFFSDSQQ
                     KQQLQITSDYTFLINGQSLGTSVVEIDEYRLVVRDQYGFYLIFSYPEQTLYDESADTS
                     YKLLSEK"
     gene            90094..90822
                     /gene="sfsA"
                     /locus_tag="SCJ86_000200"
     CDS             90094..90822
                     /gene="sfsA"
                     /locus_tag="SCJ86_000200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325468.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA/RNA nuclease SfsA"
                     /protein_id="HEG1167795.1"
                     /translation="MKYQQVEIAFFIDRPNRFIAHCMKKDGEIIIAHVKNTGRGKEVL
                     IPGAEVAIVFAPGPKRKTAYDLIAVKKKGDWFNIDSQLPNRLAIDGILDGTILLPGLT
                     GPFTVYKREVTYLHSKFDIYLETVVGQKAFVEVKGMTLENKAIGAFPDAPTLRGLKHI
                     GELTYAAQDGYAAYVLFIAQFEHLHLATIHEEMQPALADMVRHAQQSGVQILAYNCQV
                     SADEVTVKQAIPFDVDIPFEDPIQ"
     gene            90838..91851
                     /locus_tag="SCJ86_000201"
     CDS             90838..91851
                     /locus_tag="SCJ86_000201"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631998.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HEG1167796.1"
                     /translation="MINLGIIGTNWITHQFVQAALSTKKYELTAVYSRHLEKAQEFGS
                     HYEGDIEFATDLKTFFNITHMDTIYIASPNSLHFSQAKQAILAGKNVIVEKPAFSTPK
                     EMDEIIHLANEHNVYFFEAARNIHESGFKKIADYLPLKDEIIGANFSYMKYSSRYDQV
                     LDGEEPNIFSTHFSGGALMDLGVYLVYAAVAWFGMPKEVHYFPRKISTGVDGLGWGIL
                     RYENFDVSIQPGKIGDSYLPSEIYFDSGTLVMNGVNAIEQAQFHDRAHKEIIDLAVTA
                     AENPMADEAEDFANVMAHPKDPAWGLLYEEWIELARNVNQVIYDLRKNGGIKFDADNE
                     EDF"
     gene            91851..93128
                     /locus_tag="SCJ86_000202"
     CDS             91851..93128
                     /locus_tag="SCJ86_000202"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294535.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HEG1167797.1"
                     /translation="MNEFVQVFPENWQKKYEDKGFISPSLIQRAVFQPLSNKQSIVAV
                     SPTGSGKTLAYLWPLLLNVEPGEASALVIFASSQELAIQVADVAREWGKDKELKVQSL
                     VGGANVKRQIEGLKEKPEILIGTPGRILELMKAKKIKAHQVKTMVFDEADQLFDAGNS
                     QIIDQILHQAPTEYQLAFFSATADRSLESIEKITGKTFETIDVTAEDDSRKGLRHYFL
                     RVPIRKKDDYLRRLTHIEDFHGLIFFNQLTELGVMEEKLLYRGVPVGSLASDQNKLLR
                     KAALEQFKKKMVRALLTTDVAARGLDITGLPYVVNAEVPLSEEAYLHRSGRTGRMGNE
                     GIVITLVQDNNLRDLKRLLRPSNITLEEIFLYGGKLQTEQPTKEDAAPAVNKKYAKTF
                     SGKEQNPEYNKEKIRKKVKNKSKKERNKGARRK"
     gene            93191..95893
                     /locus_tag="SCJ86_000203"
     CDS             93191..95893
                     /locus_tag="SCJ86_000203"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma 54-interacting transcriptional regulator"
                     /protein_id="HEG1167798.1"
                     /translation="MKERQEEILQLLTENQQGLTASDVAERLTIDRSNASRYLSELYK
                     AHHIVKTAGRPVVYSLPTEKSKSDEVHVDSSTQVTFETLVGENDSLKVSIQQAKAAIL
                     YPPRGLHTIIFGETGTGKSMFAECMYHFAIDSEMLSADAPFVSFNCADYAQNPQLLFG
                     HIFGIKKGAYTGAAQDSPGLIAKADGGILFLDEIHRLPPEGQEMLFTFIDKGIYRPLG
                     ESAQVHEASVQIIGATTESSESFLSTFNRRIPMAITLPNLASRSLDERYEIISLFIKQ
                     EANRLNQRIDIEKEAILAFMLYDAEGNIGQVKRDLKLVCAKSFLHYRTHHEKKLIIRK
                     EELSLQVQKGLLKIKEIPERLDRFMDSKSQYLTFEPGFADVVWSQDPERNMQVYNDIE
                     EKVTSLSETGVENIDLETLISKDVDAYFQTYVKELTKSSIPKDLLPDEIWRLTNQLYD
                     VAEKRLDRIYNEKARFAFALHLQSTLDRVREGHMIVHPDLNNVRKNFKREFQVAIDLS
                     TIIEEEEQVEIPFDEIGFISMFLSINLGESEPVKENKVDVLILMHGRQTASSMLEMAQ
                     ELLGTKVGKAFNMPLVMEVQTMYEEVLQYVKNYQEHLTNGLLLLTDMGSLNTFSDLIY
                     EETGIRTKAITMTSTMIVIEAIRMASVGRSLEDIYQNIQLSFESIVRDQFKAEKSETL
                     QRKKAVIVTCFTGEGVAAKLYQRIMPVINQNKVELIQMQFLERESFKKHIDALLEEYE
                     IKAIAGTVDVDYQNIPFFSAYDVFDDEKLNILKRIADDEIPTAKIVDSLAGSLSDVFS
                     IDELIRSLQKTVQQIQTDLSIIVEPGVDTGIVIHLAFMLDGLIKQESHRQFEKLSIFS
                     KIYRLEMDIVRANLMALERKYQVKIPEDDIAFLTQMFLENKIKK"
     gene            96045..96362
                     /locus_tag="SCJ86_000204"
     CDS             96045..96362
                     /locus_tag="SCJ86_000204"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HEG1167799.1"
                     /translation="MAKKTIMLVCSAGMSTSLLVTKMQKAAEEKGMEADIFAVSASDA
                     DNNLESKNVDVLLLGPQVRFMKAQFEQKLAPKGIPLDVINMQDYGMMNGEKVLAQAEN
                     LMK"
     gene            96392..96712
                     /locus_tag="SCJ86_000205"
     CDS             96392..96712
                     /locus_tag="SCJ86_000205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328204.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS lactose/cellobiose transporter subunit IIA"
                     /protein_id="HEG1167800.1"
                     /translation="MEDQQNLEAIMGLIMFGGNAKSDAMEAIAAAKIGDFELADRKIA
                     DAEESLIQAHHAQTDMLTQEAQGNHMQVTLLTVHSQDHLMTSIAFTDLAKEIIELYRR
                     VYSE"
     gene            96820..98280
                     /locus_tag="SCJ86_000206"
     CDS             96820..98280
                     /locus_tag="SCJ86_000206"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343028.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HEG1167801.1"
                     /translation="MNGLSSWMEKYILPVAAKIGAQKHLVALRDAFIGTLPATMAGSV
                     AVMINAIIRDLPQQFNSTYADSLAADKGIFAVLNVIIGINGFVWNGTLAIAGLIFAFS
                     WGYNLARAYGVNDLAGGIVGLATLIQGISFGPALSAALDVNVPSNVADAINSSEIGAT
                     IADGTLSVGLWGWLKLDHLNGNAYFTVMIMGAISVIIFCKLMLANITIKMPDSVPPAV
                     SKAFAAILPATIALYVIAIINYIVGRVADGQLIIDLVQKYIAEPFLGLSQGLGAVLIV
                     TVFVQVFWFFGIHGPNVLAPVLEGIWGQAQLINIDIFQKGYNGKTGTPAVLDAIEDGK
                     AYMWVRGSFDAFAWFGGSGGTIVLLIAILIFSKRADYLTVAKLSIGPGIFNINEPVMF
                     GLPIVLNAIMFIPFLIAPVVATAIGWTATYLGLVAPVSQQVTWVVPPFLLSFLATGAD
                     WRAPIVTLVCMVVTFLIWAPFVISANKMDPAAGEQQ"
     gene            98348..98569
                     /locus_tag="SCJ86_000207"
     CDS             98348..98569
                     /locus_tag="SCJ86_000207"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297972.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEG1167802.1"
                     /translation="MITIASAMQQDEIKELLEGYNEEGMTFTFKEKQGIKLFFETSAE
                     DPEVAAKKAKELIKAQPWGTVLYFQSTAV"
     gene            98600..98782
                     /locus_tag="SCJ86_000208"
     CDS             98600..98782
                     /locus_tag="SCJ86_000208"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3188 domain-containing protein"
                     /protein_id="HEG1167803.1"
                     /translation="MIKNGLFLCSIGSLIVLYSLNPHSQRYDLYSMVTGLFLTGLGIY
                     FFMKGKKREEAKEKNN"
     gene            98782..99195
                     /locus_tag="SCJ86_000209"
     CDS             98782..99195
                     /locus_tag="SCJ86_000209"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315398.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3284 domain-containing protein"
                     /protein_id="HEG1167804.1"
                     /translation="MEITKKLNIPASFFYDKVMDSVLFDVRKATGKSVTRKQLKNLEY
                     VKQFSQNNRARIKIEEVIENQSYKFRTSTTKNEFVVHYQIKPLDDSTCEVHYTEKMES
                     YGMLQKLNDMLLGTILMYFKRRRFKKMLDMIEATY"
     gene            99318..100499
                     /locus_tag="SCJ86_000210"
     CDS             99318..100499
                     /locus_tag="SCJ86_000210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEG1167805.1"
                     /translation="MTNKVRELGVSSIEEMFDLATYAFNGADTPERRERFRTLAENSW
                     NYGFFDEEDRLTSQVMATPFPVNFYGQEYLMAGIGYVASYPEARGQGGINQIMEKILE
                     DCRDRKVSLSYLAPFSYPFYRRYGYEQSFDKISYQLNSRDVPFIKKKSGKIKRMDFED
                     AKAAIRRIYEQMPENQRAGLKREDWWYTYKFKQKGNYQFALHFDDNEEATGYIVYQLA
                     TSSFIIAEWGFLDHDAFCSLVRFVSSHNGAFETFEYSCGYGGNDQNYLLENPFASVMI
                     TPYMMARIVDISLFLENYPFKKRQAAFALQIEEDHYAKWNEGIVEVTIENGKNHLSKV
                     EQTKLPIVRGSIQHITQLLLGYRKVEELVFQERIQVTKEYEETLSSILPQQQPILNDY
                     F"
     gene            complement(100570..100734)
                     /locus_tag="SCJ86_000211"
     CDS             complement(100570..100734)
                     /locus_tag="SCJ86_000211"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286676.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEG1167806.1"
                     /translation="MNDKPKEKVYAERLDSSKKNREKITKEAIQDHKKENPKTKENKK
                     FKFTKKEDLL"
     gene            100888..100961
                     /locus_tag="SCJ86_000212"
     tRNA            100888..100961
                     /locus_tag="SCJ86_000212"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:100922..100924,aa:Arg,seq:cct)
     gene            complement(100998..101198)
                     /locus_tag="SCJ86_000213"
     CDS             complement(100998..101198)
                     /locus_tag="SCJ86_000213"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286470.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HEG1167807.1"
                     /translation="MKINYTVDLVSFSLVINFSLFSLRLFIDGLRHTHASILLYQGVN
                     ILSVSKRLGHSSLETTMSTYTK"
     gene            complement(101503..102735)
                     /locus_tag="SCJ86_000214"
     CDS             complement(101503..102735)
                     /locus_tag="SCJ86_000214"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314209.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase"
                     /protein_id="HEG1167808.1"
                     /translation="MLSDFNELLKKYARLIAETGVATEKGHTVVLQISVEQAPLARLI
                     TQEAYKLGAAEVIVQWTDDQIQREFLLHAATDRIENVPQSKIDQADEWLEKGASRISV
                     VSADPDAFAGVDSHRVASYQSANGKALMNLRKATQANKVSWTVVAAAGKQWAAKVFPE
                     LPEEKQVEALWDQIFKTTRVYEEDPVLAWKKHDEKLAKKAEELNREQFSALHYTAPGT
                     DIIIGLPKNHLWEGAGSYNARGEKFMANMPTEEVFTAPDSHRVDGYISSTKPLSYAGT
                     IISGMKFTFKDGKVVDFSAEQGEDVLAKLLDTDEGARRLGEVALVPDPSPISQSGIIF
                     FNTLFDENASNHLALGSAYAFSVKGGTEMSDEELAEAGLNRSQTHVDFMVGSDKMDID
                     GIREDGSTVPIFRNGDWA"
     gene            102989..103558
                     /locus_tag="SCJ86_000215"
     CDS             102989..103558
                     /locus_tag="SCJ86_000215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339630.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HEG1167809.1"
                     /translation="MARKKTITREQILAAAYEVAATDGFTRFTARNIANKMNCSTQPI
                     YLEFKNMDDLRQALFEQIYDHLQYEVFTVEHTGNTIIDLAFNYIHFARREKKLYRSLY
                     LEEYGGGKEMQNFSYTYFVNTVKRDPAYVNLSEEQIESLYNGTWIIATGIAALMSSSI
                     IHPTDEQIEKMIQDSIDAILKFKEPIEID"
     gene            complement(103736..104176)
                     /locus_tag="SCJ86_000216"
     CDS             complement(103736..104176)
                     /locus_tag="SCJ86_000216"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297963.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flavodoxin"
                     /protein_id="HEG1167810.1"
                     /translation="MALAKIVYASMTGNTEEIADIVAEAFENLDIEVEINECTQVDAD
                     EFEEADICVVATYTYDDGDLPDEIVDFYEDLQELDLLGKIYGVCGSGDTFYDEFCKSV
                     DDFDAAFAKTGASKGAENVKVDLNAEEEDIENLEAFAKELVAKI"
     gene            104334..105098
                     /gene="map"
                     /locus_tag="SCJ86_000217"
     CDS             104334..105098
                     /gene="map"
                     /locus_tag="SCJ86_000217"
                     /EC_number="3.4.11.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008377375.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I methionyl aminopeptidase"
                     /protein_id="HEG1167811.1"
                     /translation="MITLKSAREIEMMAESGALLADVHKNLRDFIKPGITSWDIEVFV
                     RNYIESHGGIAAQIGFEGYEYATCISINDEICHGFPRKKPLKNGDLVKVDMCIDLKGG
                     ISDSCWAYVVGESTPEIDRLMEVTKKALYIGIEQAQVGNRIGDIGHAIQTYVEGENLA
                     IVRDFIGHGVGPTIHEEPAVPHYGEAGKGLRLKEGMVITIEPMVNTGTWKMKMDPNGW
                     TAYTRDGGLSCQYEHTLAITKDGPRILTSQGEEGTY"
     gene            105130..106053
                     /locus_tag="SCJ86_000218"
     CDS             105130..106053
                     /locus_tag="SCJ86_000218"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330638.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YihY/virulence factor BrkB family protein"
                     /protein_id="HEG1167812.1"
                     /translation="MKVWNKIKQNENLMRFIETTQKRITDSEMSTTSVVVAYYLLLSL
                     FPLIIAFGNILPFLHIDQETVLTYLREVIPETVYQFIGPAIKDLLTQSSGGLLSVSAL
                     AALWSASQSINALQIAMNKAYGVENRKNFLIVRFFSLIVIVLFMIAISGVTIVLGLGQ
                     LILDAIQPIVQIPVNFIDQFQTLKWPITLVALFVIMFLIYLIVPNAQLKLKTIIPGTI
                     FATVGWMLLSQVFSIYARYFATRISGYQIIGSFIVLMLWLNFAATIIILGGIINAVVQ
                     EYVTGHQIKKRRDPSRKWFNKLKTKFSHKKK"
     gene            106129..107268
                     /locus_tag="SCJ86_000219"
     CDS             106129..107268
                     /locus_tag="SCJ86_000219"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329034.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="undecaprenyl/decaprenyl-phosphate
                     alpha-N-acetylglucosaminyl 1-phosphate transferase"
                     /protein_id="HEG1167813.1"
                     /translation="MLVSFSMVIRFFLTILLSLILTPVFKIISVQTGMVDKPNERRIN
                     KVPMPSAGGLPIFISFVISTLFLFRKIIPQSYIVPILIASLVIIVTGVLDDKYELTPK
                     QKSIGILIASLIIYFVADIRIDSFTLPFIGYIQLGWLSFPVTIFWIFGITNAVNLIDG
                     LDGLAAGISLIGLITIGIIGYFFLHASTVYIPVVIFCLVASIIGFFPYNFYPAKIYLG
                     DTGALFLGFMMAVLSLQGLKNVTFVSSISLLIVMGVPVTDTFFAIIRRKANRVSFSTA
                     DKKHLHHRLISLGFTHKGAVLTIYAMALMFSFTAMVMNYTGRIGTIVLIIAMLFAAIL
                     LFELIGLINEKHQFILKTLRFLGNKEYRTQVKQKYLKKWNRHIDE"
     gene            107261..108061
                     /locus_tag="SCJ86_000220"
     CDS             107261..108061
                     /locus_tag="SCJ86_000220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346778.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HEG1167814.1"
                     /translation="MNNKLNQPILISIVTANSDKIFRTLDNFISTVKDETAVKIRIFD
                     NNSLPEYKKRLTEYRKYPYIDIYFHDENSGFGYGHNHNLIASDSRYGIICNPDILVDE
                     ETVTALVLLLKEHPECAMLAPKILNEDGTTQHLIREQLAVFDYVLRFVPFKWVKKKFD
                     KRLAVFECRGLPTDANSYVRMISGSFMVADIDKFKEIKGFDERFFMYFEDNDLCLSFE
                     KAGYKLLYTPFYAVIHFYGKGAHRNAKLFRVFLTSMMKFFNKWGWRFF"
     gene            108061..108888
                     /locus_tag="SCJ86_000221"
     CDS             108061..108888
                     /locus_tag="SCJ86_000221"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377110.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HEG1167815.1"
                     /translation="MKPEILVVVVLYQQLFSQSPAYGPLSKALSEKQIQLIIYDNSPQ
                     RQQNELFEKENIVYHHDPQNPGLAVAYNFALSQADEGTRCLVTLDQDTRLVDDYFELL
                     RKVTFTDECVAAVPMIFSGSRQVSPLYSDRYINRTAQAVEVEGPTAERIMAINSGVAW
                     SVKFLKKIGGFNPAFSLDFLDHWLFWKVDQLEKTVEILPVRMEHDLSVLDYEKVSSNR
                     YRSILQAENRFYHEYDQRHLSSHRRQLLLRTAKQFLTVKNRHIWRMTLRSYVKNWKV"
     gene            108866..109600
                     /locus_tag="SCJ86_000222"
     CDS             108866..109600
                     /locus_tag="SCJ86_000222"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347956.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HEG1167816.1"
                     /translation="MLRIGRCEFVRSVCIATFNGGRYLREQLDSILSQLSAEDEVVIS
                     DDGSVDDTWDILTEYAEKDKRIQLFSGPRQGLIANFGYAIQQTKGDLIFLADQDDVWL
                     ETKVKKITDYFEQNPDQLVVVSDLIIVDSELQEIHDSYFSFRNSKEGWLNNVIRSHYI
                     GAGMAFRSSLKPDILPIPSEVPMHDMWIGLLGGKKTGFLREPLTLYRRHDLNASEIET
                     HSKLRQKISWRVHLITALIKRKWFKR"
     gene            109700..110566
                     /gene="rfbA"
                     /locus_tag="SCJ86_000223"
     CDS             109700..110566
                     /gene="rfbA"
                     /locus_tag="SCJ86_000223"
                     /EC_number="2.7.7.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735945.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-1-phosphate thymidylyltransferase RfbA"
                     /protein_id="HEG1167817.1"
                     /translation="MKGIILAGGSGTRLYPLTKATSKQLMPIYDKPMIYYPMSTLMLA
                     GIKEILIISTPQDTPRFKELFGNGNDLGLQIEYAVQESPDGLAQAFIIGEEFIGDDSV
                     CLVLGDNIYYGGGLSKMLQRAASKDTGATVFGYHVNDPERFGVVEFDENMQALSIEEK
                     PAQPKSNYAVTGLYFYDNEVISIAKGIKPSERGELEITDVNKAYLDKGKLSVELMGRG
                     FAWLDTGTHESLLEASTFIETIEKRQNLKVACLEEIAYRMGYIDKEQLLALAQPLKKN
                     QYGQYLLNLAAE"
     gene            110580..111152
                     /gene="rfbC"
                     /locus_tag="SCJ86_000224"
     CDS             110580..111152
                     /gene="rfbC"
                     /locus_tag="SCJ86_000224"
                     /EC_number="5.1.3.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010771301.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-4-dehydrorhamnose 3,5-epimerase"
                     /protein_id="HEG1167818.1"
                     /translation="MKVTDTKLTDVKIVEMDVFGDHRGFFTESYSKAKFAEHGLKYDF
                     VQDNHSLSAEAGVLRGLHFQKGEAAQTKLIRVVTGAVLDVVVDIRKGSPTYGQWDGFI
                     LSEHNHRQLLVPKGYAHGFVTLTPNVNFLYKCDNYYNAEADGGIAFDDPDLAIDWPID
                     PSRAIMSEKDHRHPTLKEFEAENPFVYGEI"
     gene            111174..112202
                     /gene="rfbB"
                     /locus_tag="SCJ86_000225"
     CDS             111174..112202
                     /gene="rfbB"
                     /locus_tag="SCJ86_000225"
                     /EC_number="4.2.1.46"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010719743.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-glucose 4,6-dehydratase"
                     /protein_id="HEG1167819.1"
                     /translation="MKNIIVTGGAGFIGSNFVHYVVNNHPEVHVTVLDKLTYAGNKEN
                     LAGLPSDRVELVVGDVADAELVDRLVKEADAVVHYAAESHNDNSLKDPFPFVQTNIIG
                     TYTLLEACRKYDVRYHHVSTDEVYGDLPLREDLPGHGEGEGEKFTAETPYNPSSPYSS
                     TKAGSDLLVKAWVRSFGLRATISNCSNNYGPYQHIEKFIPRQVTSILSGIRPKLYGEG
                     KNVRDWIHTNDHSSAVWLILTKGRIGETYLIGADGEEDNKTVMELILEMMGQPKDAYD
                     HVNDRAGHDLRYAIDSTKLREELGWQPEFTNFRDGLADTIKWYEEHEDWWKKEKEAVE
                     AAYAKNGQ"
     gene            112300..113151
                     /gene="rfbD"
                     /locus_tag="SCJ86_000226"
     CDS             112300..113151
                     /gene="rfbD"
                     /locus_tag="SCJ86_000226"
                     /EC_number="1.1.1.133"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-4-dehydrorhamnose reductase"
                     /protein_id="HEG1167820.1"
                     /translation="MFLLTGGNGQLGTELRHLLDEKNLPYVSTDAKDLDITDAEKTMA
                     YITELKPDVIFHCAAYTAVDKAEEEAKELDEKINVDGTRNVALAAKAVNAQFVYISTD
                     YVFDGKNKNVEYEVDDQTNPLNEYGRTKLLGEKAVEEILEDYYIIRTSWVFGIYGHNF
                     VFTMQRLAETRDKLTVVNDQFGRPTWTRTLAEFMVYVIEEKAPFGIYHLSNENSCSWY
                     EFAKEILKDTDVEVAPVTSEEFPQKATRPQYSVMSLKKTEALGFVIPTWQEALAQMLE
                     AAKKMNK"
     gene            113186..115219
                     /locus_tag="SCJ86_000227"
     CDS             113186..115219
                     /locus_tag="SCJ86_000227"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377112.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEG1167821.1"
                     /translation="MEKQTRSARRKEKPKANQADSNLVLDLFLKLRYLIGVLVIVVIV
                     TFNLNGSSLGVWDKYVSQRDDGKKTDVIFGQNREVRSDEWLVQTPFYLSQAEEDFSLV
                     NEDYSLNGQNMIIAYNSPVKDITVIGKPFNWGFFFLGRDRGLSFYWAMKLVVMVLLGF
                     EVLMILTKRKKALSLIGAFALTFSPAVQWWFMQHVGDLIFFTLGLMIAFYHYFYNHDK
                     KWLRTLMMLLVVIFGIGFILVIYPAHQVMLAYLLIFYFVGLLIYYSKKVSWDLLDVVL
                     IGGAVLFIGGVMVHFWLTSKDALLASLNTLYPGKRVSTGGKWTIGEFFSFLTNWKIPF
                     EDINYSNNSEVALFYHFFPTVFLASPFVLFGKKDSEQKLLGRILMIFCLFSIFWITVG
                     LPKWLAEITLLSYVPPLRAILTFSFAACLLSIWFIAYIWREDVIPIWYKFILLAVNAW
                     SYFYAITHSKMDNYFSDFETIAVAVLGTVILAFALFGWRRLFYLAFVALVIVSGFFVN
                     PVVEGTGAIFEKTLAKEIQKIDQQDPGKVWLSEDELYNFTPTLGVKSFNTVRFYPDIA
                     AWKKIDPKEEYEKYYNRYAHTRAFIAKEDTKFILDRPDMFSVTLNFEDAEKLGIDYVI
                     SKRPLTDYNQLYEAQFTQLYGPDKDGYMIFKVEYPDYPDVPQTQTQENQLVVQ"
     gene            115263..116543
                     /locus_tag="SCJ86_000228"
     CDS             115263..116543
                     /locus_tag="SCJ86_000228"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377113.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EpaQ family protein"
                     /protein_id="HEG1167822.1"
                     /translation="MKIIQAIVQKFVNKMSVGFLLLLLTLVFFVWTAGVGFPATLFIY
                     NNSSLLIAFSVAILLILNVYKAKGVDFLYLGVALALFIFFSYFRELRNESLFGDLLFP
                     LVILFCLVIKWIEWTKWDRGIYLIVFTVFLGITLYRVFTEIQVPEGQSIWAPGNSLSD
                     IWINTNTIGSSLMTLALLISGFASSFERWYVRIIGGIAVVAAFLGIWVCQSRGALVAL
                     IVFAILDLLPKSLMRVIRAPFIAYTVTILLALPISYLAAVSEKVNLFTDREDIWHKFY
                     QTLGEKSEQVLLGMKTFIFQRGNQFLGNHNSYNSILNIYGLIGFGIAALLLILFIGRL
                     TLKADLSNGQMTFIWAFFAVMMQSFMEDTLTSYPWVPIIYILLAMAVHRYDEPEVAEE
                     FYVMEEAYDDMTYDDTSYEEQPDSAHSSRIQRYH"
     gene            116753..117559
                     /locus_tag="SCJ86_000229"
     CDS             116753..117559
                     /locus_tag="SCJ86_000229"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347068.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEG1167823.1"
                     /translation="MFKDLYLFMKDIYQNRKLLLQFSINDFKSRYAGSFLGILWAFIN
                     PLFTVLIYWLVFGVGLKAQMIDAKYPFIVYLVTGMIPWFFFSDAFASTTVVFREYTYL
                     VKKVVFNIRILPTTKILSNLYTHLFFILIGIAVALINGIYPSAMSLQLIYYLFCMCAF
                     LTGLTWLTASIQPFLPDIMQLITIVLQLIMWTLPILWSPNQFDSGLVSILKLNPLYYV
                     VQGYRESFLSEAWFWEHGTYTLYFWVFTLVMFVIGSAVFRRLKPHFSDVL"
     gene            117571..118791
                     /locus_tag="SCJ86_000230"
     CDS             117571..118791
                     /locus_tag="SCJ86_000230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEG1167824.1"
                     /translation="MTEYAIRLKDITKSYNMYAKPSDRFREALNPFKKSYHDVFYALK
                     NVNVEIKKGEMIGFVGENGSGKSTMLKIITGVLTPTSGTMEIEGKISALLELGSGFNP
                     EYSGYENIYLNGMVLGFSREEVDEMVDDIIEFADIGDHIYQPVKTYSSGMFVRLAFAV
                     AINVNPDILIVDEALAVGDLEFQLKCMEKFTEIKNSGKTILFVSHDISSIRRFCDRSY
                     WLKNGEVVEYGDTMEVTTNYENFLKKKSVKTVDRNKNQVERFAAPDIVDVTKAELLDK
                     DKNPVDMLEQGEKLYVKVTYEVKDDTIKNPVLGIALRTVDNFYVCGLNTLLDGIKIPW
                     KKGENIFYLEYEKLGLLAGEYYFDVAVFEENATVPLVYKTKYMNLFVSGSYIGEGIVV
                     LDHKWEEGTHSNEI"
     gene            118781..120367
                     /locus_tag="SCJ86_000231"
     CDS             118781..120367
                     /locus_tag="SCJ86_000231"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328620.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HEG1167825.1"
                     /translation="MKYDFEMDLDEQSSVGKIAAQIKPGSKVLEFGPGNGRLTKHLIG
                     AKQCEVSIVELDKELFDFVSEFAQDGFYGDIESFEWANYYAGQTFDYVLFADVLEHLV
                     DPGKTLKKVREFLNEEGEILITFPNLAHNSVMIDLFNNQLPWASYGLLDETHNSFYTH
                     DGFQKVFEKAGLFINIEDYLYLAVGDTELKSTYEELPEAVRYDFKMRPFGEVYQYFFS
                     LMKHPVAQSSIAEPQNSNYVKVLEVTQQTKQDETIQQIPFNNFTGENETLSFPVSETT
                     ERMVFRFAQQPSFIEFSAEAAGEKLTFIDSNAVVKTVNDCYLFDGKELPEFVLTDISG
                     KEVTIHCHYRFIGELTPTMKELLETIRPMAEVTKQLSEKNDELERENHHLLEENTRLD
                     HTLKTTTNRYCTLLESDEWTIKHRLGRRKKETSKKIQEKELSICIDEKIWDAETKILK
                     IIGWGIANSDRQPLSYKLSADQSPFFEAIPVSREEVNQAEQLAKGTEAGFELRILCEQ
                     ERSFLVEAVAQNGQSWIIEM"
     gene            120406..122544
                     /locus_tag="SCJ86_000232"
     CDS             120406..122544
                     /locus_tag="SCJ86_000232"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328200.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HEG1167826.1"
                     /translation="MQKKEIAVFIDQITRERATGDLLIVGWAIDEVTKEIPTIKVEKE
                     NVIAEATHVVRLDINHLYNLDVKTQSGFKVRLSGKMRGKAILDFQTARHQNGIAVKLN
                     GKYPYDDGIESSWERKKRLLKKGINYARTHGVKKAIRRVKLELKPGSIDYAEWISRHE
                     KPDLKEQRKEAQGFAYRPLISILMPVYNVEIKWLEKCIDSVLDQTYDHWELCISDDAS
                     TDPAIRKCLESYQAKDDRIKVVFRKENGHISLATNSALEIAEGEFIALLDNDDELPPF
                     ALYEVAKVLNVHPELDVIYSDEDKIDADGNRFDPHFKADWSPDTLMGNNYISHLGVYR
                     TSIVKELGGFRKGYEGSQDYDLVLRVTEQIPADHIYHIDRVLYHWRTIPGSTASNGEA
                     KSYIYDSGVKALTDALSRRNIKGSVRPGRISGFYEIAYDVLQEDLVSVIIPTKNGYED
                     LKTCVDSIIEKTSYSNYEIIIADNGSTDPKMQELFAEYKHQLKDRFIVELIDIPFNYS
                     RINNLAAEKANGKYFLFLNNDTEVIEPDWMTVMVSYAQFDRIGCVGAKLFYPDDTTQH
                     AGVLLGIGGVAGHALNNYDRTHCGYFGRLVIDVNYLAVTAACMMVKAADFNAVNGFDE
                     TLEVAFNDVDLCLKVYELGRYNVYAHQAELYHFESKSRGYEDTPEKQRRFAGEIKKMQ
                     DKWPAYIAHDPFYNDNLTKDGIGDFSLRPD"
     gene            complement(122912..123895)
                     /locus_tag="SCJ86_000233"
     CDS             complement(122912..123895)
                     /locus_tag="SCJ86_000233"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177086.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydrolase"
                     /protein_id="HEG1167827.1"
                     /translation="MNKQELHDFIGFRQPNICQIVAYKDGVEVYTDEWNGYKKDDTCH
                     IMSATKSIVSLLIGIALEQGLIKSIDDKVLDYFPEYKVKRGEKTIYAITIKHLLTMKA
                     PYKCKGDPWTKVCSSEDWTKTSLDLLGGRKGITGEFNYQTVCLHILTGLLYQTSKLTT
                     VDFANKYLFDPIGIPNHVNYYAETAEEHKQFTMDKSPKTNVWFCDPLNIGTAGYGLCL
                     SASDLAKIGVLCLNKGMYKGKQIVPPMWINEMFKPRTSALEDLQNMRYGYLWWVLDSE
                     NEIYSAIGNSGNVLYIDAKNNLVISITSYFKPMIIDRVAFVEEYLKPFILK"
     gene            124273..125664
                     /locus_tag="SCJ86_000234"
     CDS             124273..125664
                     /locus_tag="SCJ86_000234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738174.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar transferase"
                     /protein_id="HEG1167828.1"
                     /translation="MNKNGEWSAARRIFIILMDVIVYNAAIYSSFLLKFRGEIPLRNL
                     ETFQHSAIIISVIFIALNILLGAYVFYNRMISDIVFVTVIGQVLMTLGIMVVTFAGRW
                     FAFPRLVILLSFVLGTLFLSIYRILVYKLYLRVSRDKKVVIIGLEKDVAPAIQNFGSK
                     KNNKHKVEAVILDNYYANIKNMIKDIDIVYLASHIEETEKIKIYQLVTKNEKKLFLNT
                     TFENLTLINPNIMNFEDESIIEASGFRIQPEYELFKRLFDIIVSLVLLVIASPFMLFT
                     AILVKVTSPGPIIYKQVRITKNQKEFSIYKFRSMTATAEAKSGPVLAKSNDARVTPVG
                     KFIRAVRFDELPQIFNVLKGDMSIVGPRPERSFFVDQFNAENPYYYLRHNVRAGITGY
                     AQVYGKYVSDYNSKLRFDLLYIKKYSLMLDLKILLQTIKILFDKMSSQGLEEDENVIS
                     LAEIAFPNEKIFR"
     gene            125680..126720
                     /locus_tag="SCJ86_000235"
     CDS             125680..126720
                     /locus_tag="SCJ86_000235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289071.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HEG1167829.1"
                     /translation="MNNPKFSIIVPVYNAEATIGRTIDILKELDYEEYEVVLVNDGST
                     DQTQEIIQELIENDERFELITTTNQGPGLARNEGIRQSKGEYLLFFDADDTPVKTILS
                     DYDHLLEKSPKADLVVSSFVFRTMDEGRIVSEKENLVTECHYLTHEEFIKDMYQLMNE
                     QLMYVVWNKCYRSDILKNNQILFKTYNSCEDRIFNLHYYEYCHQVIMNPKVEYIYEFE
                     GGKGITNQYRPNKFETFKEFYSLANTVTDDINKDGMAALLLKGTTSVIFSIYSTSART
                     SKEKKDEAKQILSDPVIREAKQIAKTDSSAKKITKLLYNMPAPLFLSAVKVGSFVEVK
                     MPGLMAALKRVY"
     gene            126739..127824
                     /locus_tag="SCJ86_000236"
     CDS             126739..127824
                     /locus_tag="SCJ86_000236"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289069.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 1 protein"
                     /protein_id="HEG1167830.1"
                     /translation="MKRILHFQGRMGLGGAESFMMNLYRKIDRTNYQFDFLIYEDYAD
                     VQDYHSEIERLGGRIFVVPNPKKNIFKYLIEVNKLLKKESFSIVHNQVYFGGGINLWL
                     AKKNGIRQRIAHSHATEDGKSQNIVMNVLRKFLTKLLLQNATDYLAVSQEAGESLFQN
                     HPFEIVHNGIDLELYSKNSEAKVNKRKELDISMSTFVVGNIGRLEKQKNQSYLLEIFL
                     EILKMNSDSYLILVGEGSLRNKLKAKAVELDIEKRVKFLGERNDIPELLSVMDVFVMP
                     SLYEGLPMVGLEAQAAKLKLVLADTISKDTKLTNNVDFVSLDETPQEWAKKVIKEPYA
                     NKITQELKSYDVSYTTEQMESIYSRDH"
     gene            127857..>128454
                     /locus_tag="SCJ86_000237"
     CDS             127857..>128454
                     /locus_tag="SCJ86_000237"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289068.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyl transferase"
                     /protein_id="HEG1167831.1"
                     /translation="MKICYLILAYNDIGNLKRLVNRLNENAVFIIHIDKKTDIEPFIE
                     AFKSYDNVEFLCNKNRVKIYWGGFSIVQAEINLVKRALQNEKYLKYVLLSGADYPIKD
                     NEYIYNYFKKNSSVEFIRGIDLDQIKHKEFYYKHIDVYQKHDYPRINRNNTTAFKIFR
                     AIINRCLRMIKLPPKIRHHKFDLYHGSQWWALSKECLTE"
BASE COUNT        41821 a        21843 c        26987 g        37803 t
ORIGIN      
        1 aataaaaaca ctaataaagg atagtacagt ataataaaaa ataaaaggta tctggtacga
       61 aaatcaacta gatacctttt ttgtgttctt atttttttgt tattttataa aaaatagaga
      121 tctcatgtta gaaaacctaa catatgcagt tgacaatatt ttttttaatt ggtacacttt
      181 agccaactta taaaacgaaa ggggacgtct gatgggagaa aaggaattgc gccgatctat
      241 gtccgtattt ccaattggta cagttatgaa gctgactgat ttgtctgctc ggcaaattcg
      301 ctattatgaa gaacaagatt tagttcatcc ggaacgaagc gaaggaaacc gacgtatgta
      361 ttcgctgaat gatattgata tgttgcttga gattaaagac tatctttcag agggattgaa
      421 tatggctggg atcaaacgtg tatacgaaat ggcacaggcg aaaaaacaag aagcaaaaaa
      481 caagaagccg ttgacagatc atgacgttcg acaaattttt cgtgatgaga ttttggcgca
      541 aggtgggtta agcaaaacag gtcattatca gtcgcaagga ttgcaaagac aattctttga
      601 ctgaataaat taaaaaagga agagggatac catggtaaag aaacagataa caggtgaaga
      661 aatcaaacga atcattgagg aagaaaacgt acgattttta cgattgatgt tcacagacat
      721 cttaggtacg atcaaaaatg tagaagttcc agtgagccag atcgacaaag tattagaaaa
      781 taaaatgatg tttgacggtt cttcgattga aggatttgtt cgaattgaag aaagcgatat
      841 gtatttgtat ccagatctct ccacttggat gatcttcccg tgggaaagtg ctcatggaaa
      901 agttgctcgt ttaatttgtg atatctataa tccggatggg acaccatttg ccggcgatcc
      961 tcgcgggaat ttgaaacgag cattgaaaga tatgcgcgac cttggtttta cctctttcaa
     1021 tttaggacct gaaccagaat tcttcttatt taaattagac gaagacggtg ggatcacaac
     1081 gaccttgaat gataaaggtg gttattttga ttttgcacca acagatttag gtgaaaattg
     1141 ccgccgtgac atcgtgttgg aattagaaag tctaggcttt gaagtggaag cttcacatca
     1201 tgaagtagcg ccaggacagc atgaaattga ctttaaatat gcagatgtgg tagatgcttg
     1261 tgataatatc caaaccttca agctcgttgt caaaacgatt gcacgcaagc atggacttca
     1321 cgcgactttc atgccgaaac cgttatttgg gatcaatggt tctggtatgc attgcaatat
     1381 gtctttgttc aatgatgaag gaaatgtttt ctatgataaa gacggtgaat tggaactaag
     1441 tgaaacagct tatcatttct taggtggttt gctgaaacat gctcgtgcct acacagccgt
     1501 ttgcaatcca acagtcaatt cttataaacg tctcgttcca ggttatgaag cgcctgttta
     1561 tgtcgcatgg agtggccgta atcgttcgcc attagtacgt gtaccagaat ctcgcggttt
     1621 atccacacgt ttggaattgc ggtcagttga tccatctgca aacccatact tggcaatggc
     1681 agtattgttg caagcaggac tagacggtat ccgtaatcag atcacaccgc cagcagctgt
     1741 tgaccgtaac atttatgtaa tggatgagga agaaagagaa gctgcacata tccaggactt
     1801 accttcaact atccataatg ctatcaaaga attgcgtaag gataatgtaa tgattgacgc
     1861 attaggacaa catatcttct caaattttgt tgaagcaaaa cgtctcgaat gggcggcatt
     1921 cagacaaact gtttccgagt gggaaagaga acaatatcta gaactttatt aaaacaggaa
     1981 taccgtgaat tagaggctgt gaaatcagcg ttttgacctg agtattagag aaactaacgg
     2041 aaaaatagtt cctcatattt tgccgttttt tgagctaata ccgagggtca gctgattgaa
     2101 caccgtttag tggagaggtt gtgaaaaagc tgccctgcaa caagtaataa gaatagccaa
     2161 gcaaaaacag cttttcttgt ttttgaattg gctgaactta ttaccgcaga tgcaagcttt
     2221 tgaacaccgt ttaccaagag gttttgaaaa agacgtttag tttcgagtat taagaaaaat
     2281 tttgtgaaaa tggctttcta catttttgca taaatttggc ttaataccga gaaactggct
     2341 tttgaatacc gtttatccag aggttgtgag cctaccattt aactccgaat cacaagaaat
     2401 aatttcaaaa acagcttctc atgtttgatg gatattgtga cttgtgaccg aggagttggc
     2461 aggagaacac cgtttattta gaaataagga ggcacgactt ttgtcgcatc tccttatttt
     2521 ttacttatgt gattcgttac cttcaatgaa tccctacaag gcacttttgt tgaaaaaaag
     2581 gtcatctttg aaaaaattag tggaattgtt ttgtttttga tattatttaa tagaaaagat
     2641 atgccaaaaa tccgagaact gtggactacg ttttttacgt agaggaggta aataagggtg
     2701 aaagaaaaag attgtttcta cgataagcta tataaactgt tgctgcaagt aggaaaaagt
     2761 agttctttgt taggtgggat attatctatc ataatgtttg ttttattttt ttatctatta
     2821 tttcacttaa aatttttcag taacgatttt ctgatttgct taggtttaac gttagctatg
     2881 tttatatcaa gcttattatg tgggttaaat tttttttgca ctcataaagg acttaagaaa
     2941 taatttctca aaggatatcg aaaaagaaaa aattgtaagc ggatagtatt catttgagta
     3001 cttgactttc ccttagtcag cttgtattat acaccttggg caccctttta gagggtcaga
     3061 agtttacaga aatttcggct ccctattctt attgaagaat agggagcttt gtttattgtc
     3121 tgagactttt cctgcacata aattaaaaag gagtttttga acttatgaca aaatatattt
     3181 ttgtgaccgg cggtgtcgtt tcatcaatcg gaaaagggat tgttgcagct tctttaggac
     3241 gcttattgaa aaaccgcggc ttaaaagtaa ctatccaaaa atttgatcca tacatcaacg
     3301 tggatccggg gacgatgagc ccatatcagc acggagaagt attcgtaaca gatgatggtg
     3361 ctgaaacaga tttggatctg ggccactacg aacgttttat tgacatcaat ttgaacaaat
     3421 actccaacgt gacgacagga aaaatctatt ctgaagtttt gcgtaaagaa cgtaagggcg
     3481 aatacttggg agcaactgtt caagttattc ctcatattac aaatgagatc aaagaaaaaa
     3541 tcatgcgagc agctaaaatg actgattcag atgtgatcat tacagaagta gggggaacag
     3601 taggggatat agaatctttg ccattccttg aagcacttcg tcaaatgaaa gcagatgtcg
     3661 gtacagataa tgttatgtat atccatacaa cattgattcc ttacttgaaa gctgctggag
     3721 aaatgaaaac taaaccaacg cagcacagtg taaaagagct acgtggttta ggtatccaac
     3781 cgaatatttt ggtggtacgt acagagcaac ctgtttctca aagcgtgaaa aacaaattgg
     3841 cacaattttg tgatgtcgaa ccagaagcag ttatcgagtc gccagatgta gatacacttt
     3901 actccatccc attagtttta caagcacaag gaatggatca aattgtttgc gaccatttaa
     3961 aactagatac accagaagca gacatgacag aatggaaaga attagaagaa cgtgtcttga
     4021 acttgaagaa aaaagtacgg attgcgcttg tcggtaaata tgtcgagtta ccagatgcat
     4081 atatctcagt agttgaagca ttgaaacatt ctggtttcgc ctataattca gacatcgaga
     4141 tcgattggat caaagcacaa gaattaacga gagaaaatgt agaagaacgt ttgaaagacg
     4201 cagatggaat cttggttcct ggtggatttg gtgatcgcgg agtagaaggg aaaatcgaag
     4261 cgattcgtta tgctcgcgaa aatgacgttc cgtttttagg tatttgtctt ggtatgcaga
     4321 tggcttgtgt cgaatttgca cgtaacgtcg ttggattaga agatgcgtca tcagctgaaa
     4381 cgaatccaga tacagcaaac aatattattg atttaatggc tgatcaggaa aacatcgaga
     4441 atttgggtgg aactttacgc cttggattat acccatgtaa aatcaaaaaa ggaacgaaga
     4501 cggctgaagc atatgatgga gccgatgttg tgcaagaacg tcaccgtcac cgctatgagt
     4561 tcaataacaa atatcgtcaa ttgtttgaag aacaagggct agtcttttca ggtgtctcac
     4621 cagataaccg tttagtagaa atcgtagagc tgccagagaa aaaattcttt gtgggatgcc
     4681 aattccaccc agaattgatt tctcgtccaa atcgcccgca aaaattaatc aaagcatttg
     4741 tcggagcatc attagcagat cacgaaacaa aataattttt gaacttgata gaaagagaaa
     4801 agtagcaaga gaaggggata agcaattttc ttgtcacttt tctctttttc tgatttgtta
     4861 ttatttgtag gaaaaaagat agaaagcaaa agactttttt cgattaaagc gctattggat
     4921 attttatttt ttgcaaaaaa cttcttgaaa attttcattt tcattgattt tagtagaaaa
     4981 ctaccgaaat gtttggtaaa atagggtcgt tggttaagaa ttgtcttaac aaatttagtt
     5041 ttacaaaact taggaggaat tttattatgc cagtagtatc aggagctgaa tttttaaaag
     5101 ctgctcgtaa aggcggttac gctgtcggcg gattcaacac aaacaacttg gaatggactc
     5161 aagcaatcct tgaagcggct gaagcaaaac aagcaccagt acttatccaa acatcaatgg
     5221 gtgcagctaa atacatgggt ggttacaaaa tctgtaaaga tttaatcact gatttagttg
     5281 aagcaatgaa catcactgtt ccagttgcta tccacttaga ccatggtgat tacgaagctg
     5341 ctttggaatg tatcgaatta ggctatactt cagtaatgtt cgatggttca catctaccat
     5401 ttgaagaaaa cttgaaatta gctaaagacg ttgttgaaaa agcacatgca aaaggtatct
     5461 cagttgaatg tgaagttggt tcaatcggcg gagaagaaga cggaatcatc ggtaacggcg
     5521 aattagctga tattgaagaa tgcaaacaaa tggttgctac aggtatcgac tacttagctt
     5581 gcggtatcgg taacatccat ggtcaatatc cagaaaactg gaaaggatta gcttttgacc
     5641 acttacaagc aattgctgat gctgtaggtc cagacatgcc tttagtatta catggcggat
     5701 caggtattcc tcaagatcaa atcgaaaaag ctatctcaat gggtatctca aaagtaaatg
     5761 ttaacactga attccaatta tcatttgctg cagctactcg taaatatatc gaagctggta
     5821 aagataaaga aggtaaagga ttcgaccctc gtaaattatt agctccaggt aaagcagcaa
     5881 tcgttgaaga tgctaaatct catatcgact ggttcggttc agctaacaaa gcgtaagcat
     5941 ccacataaca gatacgatat ataggaatgg agaacgactt ttgaagtcgc tctccattct
     6001 tttttactta aaacggcaga ccatgctcgt ttcttttttt tatctatgat aaaatacaag
     6061 catatccatg aaaaaagatt aataaacaag gtggaaacag atgaaaaaaa tcgtaatcaa
     6121 aggaaaccga cctttagctg gtgaagtaac gatcagcggt gcaaaaaata gtgcagtcgc
     6181 attgattcca gctgcaatct tagctgattc cccagtgatc ttggaaggtg tgccggacat
     6241 acaagatgtt cattcattaa ttgaaatttt agagatcatg ggcgcaactg tccatttttc
     6301 caacaacata ctagaaatcg atcctcgaga agtcgtatcg attccgatgc cgcacggaaa
     6361 aatcaatagt ttgcgtgctt cttattattt tatgggaaca ctgcttggta aatttggcga
     6421 agcggtagta ggcctgccag gtggctgtta tttaggccct agaccgattg acctacacgt
     6481 caaaggattt gaagctctag gtgctgaagt gacaaatgaa catggtgcca tgtatttacg
     6541 gacagaaaat aaaggattgc gtggcaatcg gattttcatg gacgtggttt cagtcggagc
     6601 aacgatcaat gtgatgttag ctgctgtaaa agcaaaagga cagacagtaa ttgaaaacgc
     6661 tgcacgagaa ccggaaatca tagatgtggc aactttactg aataatatgg gggcaaaagt
     6721 tcgaggagcg ggtacagatg tgatccgtat cgaaggagtc gaaacattac atggctgtcg
     6781 gcatttcatg attcctgacc gcatcgaagc aggaacctat ctagcattag cagctgcagt
     6841 aggaaatggt atcaaagtga aaaatgtgat ctttgaacat ttggaaagct ttatagcaaa
     6901 attgcaggaa attggtgtcc atatgaaaat cagcgaagat gaaatcgaag tttacccctc
     6961 gaaagaatta aaacccgcaa acatcatgac ctatccttat ccaggatttg ccacagatct
     7021 gcagcagcca cttactgcgt tactattgat gactacaggc acttcagaaa tcatcgatac
     7081 gatctatgcg aaacgtgtga accatgtgcc ggaactagca cgaatgggcg ctgatattac
     7141 cgtggaagga aatatgatca tcgtgaatgg tccaaataaa cttcacggca cagaagtcgt
     7201 ggcttctgat cttcgggcag gtgcttgttt agtgattgct ggactattgg cagaagggac
     7261 aacgacgatt tacaatgtag attatatctt acgcggttat gatcatatta tcgagaaact
     7321 aacagcctta ggggcatcaa ttgaaatgat agaggaagtg ggagctgaat gagcgattat
     7381 ttgacaatgg cagaactaga gaacaaaacg ttaaaagaag tttatagtta tgccaaagaa
     7441 tttaaaattc cttattacag taagatgaat aaaaaggaat tatctctagc tgtgattcgt
     7501 gcacaagcag agaaacaagg attttattat atggaaggaa tattggatat cgttggtcaa
     7561 gatggttacg gttttttacg gccaatcaat tatggaccaa gtgcagaaga tatctatatt
     7621 tctgcctcac aaatcagacg attcagtttg agaaatgggg acaaagtggc aggaaaagcc
     7681 cgtccaccta aagaatcgga acgttattat ggtttgatgc acgtggaaag cgttaatgga
     7741 aaagatccgg aagaagcaaa agaacgtccg cattttccag ccttaacacc tctttatccg
     7801 caaagacagc ttcaactgga aacaacaccg caaagactat ctacccgaat gatcgatatc
     7861 ttttctccaa tcggttttgg tcagcgtgga ttgatcgtcg caccgccaaa agcagggaaa
     7921 acttctgtat taaaagaaat cgcaaatggt atcaccgaaa actatccgga agtagaatta
     7981 atcgttctac tgattgatga acgaccggaa gaagtaacgg atctagaacg aagcgtgaaa
     8041 ggcgacgtgg tatcttctac atttgatctg cagccgcaaa accatactcg agtttctgaa
     8101 ttagtattgg aacgcgcaat gcgtttagtc gaagataaac gagatgtcgt tatcttgatg
     8161 gatagtatta ctagacttgc tcgggcctat aacttagtcg ttccaccaag cggacgtaca
     8221 ttgagtggcg ggattgaccc agccgcattg tataaaccta agaaattttt cggggcagca
     8281 agaaacatcg aagaaggtgg cagtttgacg attttagcga ccgctttagt cgatacagga
     8341 agccgtatgg atgacgtgat ctatgaagaa ttcaaaggaa ctggaaatct agaattacaa
     8401 ctttcacgtg aattatctga acgtcggatc ttcccagcca tcgatatcaa aaaatcaggt
     8461 acccgaaaag aagaattatt gatgccaagt gaagaattag aagaaatttg gaaacttcga
     8521 aaaaatatga ctggagattc attagaatat acggaacaac tgatccgctt tttgcgtaaa
     8581 acaaaaaaca atcagcaatt tttcaaagaa tttcaagatg tctcctttgg taaaaaaaat
     8641 ccgaccagaa atctgaaaag ataattgcaa ccttgactga aacatgttaa gatgttaatg
     8701 ttgtaaatcg acaaatcaac tctggttcag caaatggagc agggcaaagg agcgaaaaaa
     8761 tcatgaaaga aaacatccat ccagattacc acccagtagt attcatggat tcaacaactg
     8821 gtttcaaatt cttgtcaggt tcaacaaaaa cttcacaaga aacagttgaa tgggaagacg
     8881 gtaatacata cccagtcatc cgtgtcgaag taacttctga ctcacatcca ttctacactg
     8941 gacgtcaaaa attcacacaa gcagacggac gtgtggaccg tttcaacaaa aaatacggtc
     9001 tcaaagacgc aaacgctgcg gaataataaa aacgctgata actcaacgtt tagcagacta
     9061 tctgataggg atagtctgtt tttttatgtt ttttgccaat ttaaaacttc gtttgatgct
     9121 gaaaaagtcg taggatcata atgcacaaaa caatgaaaaa ctagtatata gttttaatta
     9181 tccacttgat aaaaacacct tatattaacg aaatgaagag gggttatttc gaagtaaaaa
     9241 accatttacc taaaattaga agctaagctt tcctttcaag gaaggctttt tgttttgtta
     9301 tagacttgcg attgtttgtg agctagatcg ttcattttaa tgacttatac acgcggatga
     9361 tgggtctttt tcctctgttc attcataata ttgaacggta cagctagcga tgctatactg
     9421 acctcagttc caagaaaacg ttaacatttt ttgtggggga gaggtttttt atgaggtttg
     9481 atcaattgat cgcagaagaa ctgattgttt tcgatccatc gatagattca aaaggatcat
     9541 tatttgagcg aataggggat gtgttgaaaa aaacaaataa ggtaagaaat gttcaaaaag
     9601 tcgttaaggc actattcaaa cgagaggagg agatctccac aggaatagaa gatggatttg
     9661 ggattcccca tgcaaaaagc aaatatgtga caaaaccagc actgtgtttt tttcatacag
     9721 ccaaattaac ggactatagc gggatagatg aaatgccaat tgaatatatt tttgccatcg
     9781 tagttcctga aaaatcagca gatatgcatc tagagattct cagcacactc tccagacgat
     9841 tgatgaatgc agattttaga aagggaataa aagaagccac tacgccagca gatatcatca
     9901 gacagattag tcaagaatga aaatagtaga gtctttgtcc gagtatcgat agtgaagaaa
     9961 aaggaggaaa ataatcatga aaatcgttgg tgtaactgct tgcccgactg gaattgctca
    10021 tacttatatg tcggcagaaa aattagaagt aacagcaaga aatctaaacc atcaagcaaa
    10081 attcgaaaca caaggtgtaa aaacagaaaa tcagttatct gaacaagaaa tcaaagaggc
    10141 agatgcaatc attcttgcag tagataaaga aatcgaattg gaccgatttg ccgggaaaaa
    10201 agtaaagcgt gtctctacaa gccgagcaat caaagaaccg caagtagtaa tagacgaagc
    10261 attgagagat attggcgtat tcgttgtgtc taatgaaaaa gaaccagctg caaatgagaa
    10321 aaagccgaca atctataatc atttcatgaa tggtgtgaat tacatgcttc catttgtgat
    10381 cgcaggcgga atcatcatcg cactgagttt tgcctttggg attacagcgg ctgatcccaa
    10441 ctcagcagac tataatgtgt tagcagctgc attttcaagg atcggaggag atacagcgtt
    10501 tgccatgatg gttcctgcgt taggcgcggg gattgctaca tcgattgctg gaaaagcagg
    10561 ttttgcccca ggaattgttg ccggattact ggcctctaca ggtggttcag gttttcttgg
    10621 cggaatgatt ggtggcgtgc tagcagggta tatctgtgat tttctggcaa ataaaattag
    10681 cgtaaaaaaa gaattttctg ctatttatca gctgattgtc gtcccctttt tatcgatctt
    10741 aattattggg cttttgatgg tatttgtgat tgaacaaccg atcacatggg tgttggatgc
    10801 tttgacaaat tggctgaata gcttgggaaa tacatccggg ctattgttcg gattacttat
    10861 cgggatcatg atggcagctg atatgggtgg gccgatcaat aaatcgactt cgactttctc
    10921 gatcggtttg atgtcagctg gagtaaatgc accgattgct gcttgtatgg ctgcaggaat
    10981 gacacctccc ctaggtattg ctttagccac tttattattc aaacataaat ttaccaaaga
    11041 agaacggact tcaggtcaat catgctggat tttaggcgct tcgtatatta cagaaggtgc
    11101 tataccgttt gcggtatctg acccgatccg cgtgatacct agtttgatga caggttcagc
    11161 agtcgctgca gggatttcca tggcagtagg agttacttca atggcacctc atggagggat
    11221 ttgggtgatg ctaattccaa atgttatgaa caatttacct atgtatgtct tggcaattgc
    11281 atcaggtaca gtcactacag cctttatgct tggactattg aaaaaaacag tgaaagagaa
    11341 aaaacaagac aagtggacga aaaatgaaaa tctaacaaat gaggtggaag cgtaatgtta
    11401 tatacaatga aagagttatt gaaagtggct aaagagaatc aatttgcagt accagccttc
    11461 aatatttgca gctatgatat gatgaaagca atcatcgaag aagcagagag attagaagca
    11521 ccagtaatct tagaaattca tcctgatgaa atcgcctatt taagtgatga gtttgttgcg
    11581 gcagtaagag cctatgctta tacaagtaaa ctgccgatcg ttatccactt ggatcatggc
    11641 ggtacgatag aagatgtctt acgggcgatt cgaaacggct attcttccgt gatgatcgat
    11701 gcgtctttga aatcattcga cgacaatgta cagattacga aaaaagtggt tgcgttagct
    11761 catgcggtaa atgtctcagt ggaggctgag cttggaacga ttgggaataa tggttctgct
    11821 gaaggaggtg ctgaaacaat catttataca gatcctgatc aagcgaagat ttttgtgaaa
    11881 gaaacaggaa tcgatacact tgctgtagca attggaacag cgcatggctt gtatccgaaa
    11941 aacagaactc caaaattaaa tatcgactta ctgaaagaat tgaaccaaca aatcgatctt
    12001 ccatttgttt tacatggcgg ttcaggtaat ccggacgcag aagtcagcga agcagtaaaa
    12061 tacggagttg ccaaagtgaa tttgagctca gatcttaaaa gcgtcttttt caatgctttg
    12121 agagaaatat tgagtgaaaa tccatcgatg tacgagccga atgcaattta tccagcagct
    12181 aataaaaagg tacaagaagt ggtcaaacat aaaatggaaa ttttgaacac agtaggcaag
    12241 gctagtttat actaaaaata aaaatgtgtg gtcaagaggg tatgacaata gtcttgtcat
    12301 accctcttaa ccacacacga tgttcagaag tagttttttc gaaaaatttc ttcttatttc
    12361 tcgaagccaa tcacttctgt cacaacctct tttttcctgt tactatataa agtaaatggg
    12421 gtgattttat gttgaatgaa gtggagaata gacagcagga gattgtgaat tatctaaaaa
    12481 acaaccaatt tgcaagagtc accgatttga tcgagttagt aaattatagc gaagccacga
    12541 ttaaacgtga ccttgttttt ctggaaaaaa gtggattgat ccgtagagtt cgcggtggcg
    12601 ccatgcttat tgacaatcga aaaattgacg tgccttacct gatgaagatc accaaattag
    12661 aagaagaaac agaaaaacgt aagattgcag atacagcttc ttctttgatt cgagatgata
    12721 tggtcttatt cttggattcc agtacaacct ctttacactt agtcagaaac ctagcaagat
    12781 ttgatggttt gcagatcatt acgaatggca tcgtcacagc tgctatgttg agtgaatata
    12841 caacggcaaa agtatctgta ttgggagggg caattgttcc gaaacgagca acaatcaacg
    12901 gttcaaaagc ctataacgat gcattgtcat attatgcgga tttagcgata atgagttgtc
    12961 gcgggctaga tttcagccaa ggagtaacag aaactcatga aggagaggca atggtcaaaa
    13021 aagcttttag aaaacaatca gaccggcttg ccgttttagt tacacagcaa aaaattgcac
    13081 ataaattcat gcatcaagcc attgcttttc atgaaatcga ttatctggtg acaagtaaag
    13141 aactgacaaa agaacagtat gcgaaagtaa aggaacacca tatcgaatgt ctgtacggat
    13201 aaattattac gtgaatgttc taagtggctt cctcattttt agataggacc ttatctttgc
    13261 tcgcttctct cagccgatgg tataataatg aaaaggttat cttgttagat aatcttactt
    13321 ttttatccag gagaaaggag tgcagaaaat gaaacatgtg aaggcgttgg tagtgaaggc
    13381 aatcatgatc tgggcgattc tgtgggtcgt gctgacaggt ttatacggtg ttagctttat
    13441 ggattcaacg attgttgggg taattattgt agtcatgatc tatgtgctag gggatctgtt
    13501 gatcttacgt aaagtaggga acatcgcagc gacgatcgca gatgctggtt cagctgcagt
    13561 tatcttgtgg ctctatctat attttatgaa tgatacagtc gatatttgga tgaaagtatt
    13621 gattcctgct ctattgatcg gtgtggcaga atggttcttc cacaagtggt tgctaagtca
    13681 aggcatcgta ccagatgaac ggaaaatgga ataaatcaac agttaaacta ttcttatcga
    13741 taaatgaaag ctttaaaaat aaatagatgt ttaattaaga ataatcataa actattttta
    13801 ctcaaaaaac cgctcagatt attttcagat ctgaacggtt ttttttatct attcatcaag
    13861 atcaaatttc atttttcaat gattcaatca attcgccctc tgcttcatga ctatgttttc
    13921 ggccattaac aagatagagt gctgtacata tcaatggaat gactccggca atcacataca
    13981 atcctctaaa tgaagtaaga gattttagca tccccatcaa ataaggacca acgcctaagc
    14041 ctaggtctaa accaataaag taggtagata aagcaatact gattcgatgt tcgcggacga
    14101 tttttaaaca aacagcctgt ccatttgaca taaacgtacc ataacctagg ccgactaatg
    14161 ctccagagat aagcaatgtc acactattcg tcgttacact cagtaaaaat aatcctgctg
    14221 ttaagaataa atagctagga tacatcacgt attgttctcc tttgacatcg aaaatccttc
    14281 cagtcaatgg acgtgtaaat gtgatgacca aagcatatac gacgaagaaa aatgacccag
    14341 cagcaactaa gtggattact tttgtataag aagataaaaa tgataaaaca cttgaataag
    14401 aaagacccat caaaaaggca attcctgtaa tgaaaagtgc tttcttttca ataaaactat
    14461 tgatattcca tgagcgaaga gatatttttt gttcttcaga taatggaata ttttttacag
    14521 gaaagacaag gcaggcaatc gtaacaaaga agaccagtac aattgaaaac cagataatga
    14581 aacgaaaatc agtcatattc agcaaaatca ttcctaaaaa tggcccaaca gcggcagcta
    14641 aactagtact taatccataa taattgatgc cttccccttt tttagatgca gggatatatg
    14701 cagtaacgat agcatttgta gctgtagaaa ctgtaccgta gccgaatcca ttcaagaatc
    14761 gaactaaata caatgccccg atagttggcg tataaagata tgctaatgtt gaaaggagat
    14821 agaagatcgc gccgcctctt aaggtaaact tccgaccaaa tagttctaac tgtttcccca
    14881 tgaataagcg tgccagcaat gttccgatga tatagatccc agaagcaaaa cctgcttggc
    14941 tcaagctggc gtgtaaggtg tcttgcgcaa tgactgccat gatgaccatc aacagataat
    15001 aaatcaggta gacaataaaa ttgatcaatg tgattccaat aaaatattta ttaaataatt
    15061 tcttttccat gtatgttata cattcccctt aagctgaata tgcttttaat ttttgtattt
    15121 gaacttctac atcgtgataa ttggatggat agcgatcaac aagttggcga ataaataaat
    15181 ctcttgtaga cataaacaga tttaaaaatt cttcgcattc gtttgttgga ttctcatcca
    15241 ctgctttgat caattcgatc gagttttgat acgcatcatt taaaaagact aaagaagctt
    15301 tcatatcaaa tcttgtttga tatgctgttt ctaatcgatt aatgattgtg ttcatccagt
    15361 aattcatgtt ttttccctct attcttaaaa aattttactt ctgcagtata acgaataaaa
    15421 aaaaggaaaa aaatcgcata agtgatggaa aaaggtactt aacaaatgtt aatatcatta
    15481 gtaaaatacc tttggacttt aaaaagtttt aaagtactgt atgatctttt tagatgaaag
    15541 tttggatggg ggagcatcat tggatagtag agtcttaaag gaatatttgg ataaaaatga
    15601 ttttccgatt atacgcaaag gatatcataa atatttgaca ttcgaaggga tggaagattc
    15661 ccatacgtat atcttgaaag agggaacggt aaaagcaagt gttatttcaa atgatgggag
    15721 agaatttaac cttcgctata tcaaagatct tgaaatcgtc tctcttttaa aagacgaata
    15781 ttcacaattc atcgattcgc cgtataatat tcgagttgaa tcagaatatg cagagtttta
    15841 tcgaatcaat cgtgtgcgct tttggcaaaa tatcaaccaa gacccaaagc tacaacacta
    15901 tgtaaaagag tattaccgct atcgtctgct ttattcgatg aaaaaaatgc agcaaatgct
    15961 attaaacgga aagctgggag ctgtttgtac gcaactttac gaattatatg atttattcgg
    16021 tgtgcggaca gaagaaggga tgctgatcga tttttcgatc acgaatgaag aaatcgccaa
    16081 attttgtggc atctcaactg ctagcagtgt aaacagaatt ttaaaacaac tgaaaaaaga
    16141 tggtattatc accgttaaag accatcatat cgttattttg aatatggact tgttagaaga
    16201 caatattatc ttttgatgaa caaagaggtt gtgaaatcag cgttttgacc tgagtattag
    16261 agaaacaaac ggaaaaatag ttcctcatat tttgccgttt tttgagctaa taccgagggt
    16321 cagctgattg aacaccgttt accaagaggt tgtgagcctg ccgtttaact ctgagtcaca
    16381 aggaacaatt tcaaaaaaca gcttttcatg tttgatgaat attgtgactt gtgaccgagg
    16441 agttggtagg agaacaccgt ttaccaagag gttgtgaaaa agctgttctg catcaagtaa
    16501 taagaagagc caagtaaaaa cagtctctta tgtttttaac ttggctggac ttatcaccgt
    16561 agatgtaagc ttttgaacgc cgtttatttg caacaaaagg ggctgtgaca cgatttttgt
    16621 cacagccctt tttttgcgaa cattcattta gcaattttta gaaacttctg ccgccaccgc
    16681 caaaagtccc gccaccgcta gaatgggtag tacttccgcc accgccgctt ccaggaggtg
    16741 ggcttttggg gattcttctt gtcgtgacaa aagaatttgt cagacgatcg gttttttcag
    16801 ttaatttgat actggatttt tctcggaatg gatatttgta gatgcctgat ttcaattgat
    16861 aacgcgaaac agtcacaaaa taaaagacaa gactaaggat aagagccaaa acaacagcaa
    16921 tgatgatttc tactgtcgtc aaaaccttat agcgtgtaat tttgccagtt tcttcgtcta
    16981 cacgataatg tcctcctgga actccgtctt ccacatatgt tgctgattta gacaggtaag
    17041 cttttgcagc attgtaatag gcagcatttg tcatctgatc ataaacatca tctaagatcg
    17101 aatcgattct gctatcagtc aaataatcga tcatattacc agaagtcgaa atgtagattt
    17161 cacgctgtcc catatctaat agcaatactg agccattttg atcatttccg actgcgtctc
    17221 ttaagtaatt atcagcaaag tctcttggtt cttctgaatt agatgtagtc gttacgatca
    17281 agactcgtcc tttgattttt tcattcagct tttctgcctg ttgttccaaa gattggattt
    17341 gatctgttgt gaataaccca gcctcatcat ttaccgtagc tgtatctgca tgagtagcca
    17401 ccggccagcc aacacttact agcaaaagaa agaataaaaa aaactttttc atcatatcaa
    17461 gtaacctccg atcagaaaga ggatcgctag cccagcgaaa atcccaagag cggtcagtcc
    17521 caattttttc tggctgatcg gcaggacacc gctgaccttt cctgtttgtc cattcattgc
    17581 gtagtagtag acttttttac ttgagtcatt gcttcgataa gtaacaagcc ataccggcag
    17641 taatacgtaa ttatttactt cgtttgtgat tgaagcagaa gttcgcacac cggtcaatgt
    17701 tgtatatcca tttgcggtat cttttaacag cttctctgaa tagtctttta attcagattg
    17761 aatctgtgat ttgattgcct catattcgat atctcttttt tctgcttgga acccggctaa
    17821 atactggctt ttaaaatcaa ttgctttatc taacgggaaa ggttgcacgc cttcgaccat
    17881 cttttgttgc gtattttttg ataaggcatt ttttactagt tctttaaaag aaagcttgcc
    17941 tgctcttgaa acagcaaatt gcttcgtttc tgtgtattcg atgtcaccta tccgccatat
    18001 gcggataact gtaccattcg cctgcatggc accatctact tcagcatctg tagcccaata
    18061 agggaaatac acacctgtca acttatcgat ttgatcctta ttgaaaaaag ctttgggaac
    18121 aaaccatttt ttcttggtcc aagcgagaaa tctgttgatt gcttcttctt tctcgacagc
    18181 aaaaggaagt actttatttg gcaagaattc tccgcttaaa cgtcccgata gtacgacggg
    18241 attatggcag tagtaacaat aagtcgcagc agttgtcgca tcagtcacga tctctgcacc
    18301 acagttggga caaagaaaaa gatctatcgc tgcggtatta tctgcagatt cgtccgctgt
    18361 atctgaaaaa ccgcctgcct cgtcaaccgc tttgcgctcg gcttcattca tatcggcaag
    18421 ctgttcttgg gctgtaaaag taaaatcttc ggtaggtgaa ggttcttctt tgctttcttc
    18481 gccagccata cgcgcttctt tttgcttttg ctcatattgg ctgacttctt cttctgtata
    18541 gatattcagg cagtattcgc aatgaaattt ttggtctttg ggatcaaaag tcaacggacc
    18601 gccgcaatta ggacatttat gtgtaagaac atccatcagc tgcacacctt tctaaataaa
    18661 ttaattaacg gggattcctt taaacggttt accacagttt ggacagaatt tagggatgcc
    18721 gttggaaaga tccacgatct cattgcattc actgcatcgc atttgtagct taggctgaac
    18781 gttttctaca ggtttggcag ctccgcagtt ggaacagaat tttccggaat tttctgtccc
    18841 gcaaactgga catgtccaag tatccgcatt tccttgtgca gtttgttgat tttgtttttc
    18901 tgcttgttgt ttgatttgtt cttggttatt ttgagctgct tgagaaagat aagaaccatt
    18961 tgcattcatt cccataccta cacccataaa gccggtcatt gctcctgctt cattttttcc
    19021 ggctgcttcc ataccacgtg caatggaacc ttgcacgtat ccttcacgta cgctaggatc
    19081 acctagcatc gccccttcgt tacgcatatt gatcagtttc acagaatcat cggtgtaaga
    19141 aatgctggct acagccacag aaacaatctc catcccacgt aattcacgcc aagaatcatc
    19201 caatgcagta tccatgtatt tgcttaattc caaactctta gatggtacat aagaaatccg
    19261 ttgaccatct gcagacatct gattgattgc tgattgtagc gctgttagga attcagctaa
    19321 atactgctca ttgatatcat tgatttccac ttgtgttttg tttttaggga ttgcgtttgt
    19381 ataaaataaa atcgggtcaa cgacatgaat agaataagtg ccatgtgccc gtaagaataa
    19441 ttctgcgtta tagaaattgt caaagtagtt caacggcgcg cttgtaccga atttgattcc
    19501 ctttatttct tggagattga tataaaaaac ttgttgtttc tgcggtgtga cgccgccaaa
    19561 tttgaatcgg tcaaaagttt cagcaattgc ttctttcagt gttccattga acatggaagg
    19621 agctgcgtca tttttaatgg tgtaataacc ttcctcagcg gtataatcga tgatttttcc
    19681 accatcgacc aaaagcatca tcatgtttgg atagacatgg acgactgttc cgtctgttaa
    19741 aacatcttct gttcctttac gattggagcc acgtttgtca tctttgcgga ctttgacacc
    19801 ttttgtcatg accgttgtat cactcatatt gtctggttcg atgacttcta gccattgatc
    19861 tgccagaccg ccgccaacca tacttgttgc tgctttgata agacccataa aaaattcctc
    19921 ctctgattca gctagtgcca aacaattatt gtttatttat tataccatag gaagatgtga
    19981 aacttctcgg actagagacg gaattgaaat ggaaaattgc gtattgttta aatacctgaa
    20041 aaagccgaga aggatagtag tttttttacc taatatcacg tataataaga gatagtgaat
    20101 tttttaaaaa agggggatcc ttatggcata tcaagcactt tatcgagtct ggcgttcgca
    20161 gcgtttcgaa gacatcgttg gacaaaaagc tgtgacacag acactaaaaa atgctattgt
    20221 ttcccacaaa acctcacatg cctacttgtt tacaggaccc agaggaaccg gaaagacgag
    20281 tgcagcaaag attttcgcaa aagcaatcaa ctgtccgaac agtaaagacg gtgagccatg
    20341 caacgaatgc gaaatgtgtc gatcaattac tgctggtacg caagaagatg tgatcgaaat
    20401 cgatgctgcc agcaataacg gagtcgaaga aatccgattt atccgtgatc gggcaaatta
    20461 tgcgcctaca aaggcagcgt acaaagtgta cattattgat gaggtacata tgctttcaac
    20521 aggagctttc aatgccttgt tgaaaacatt ggaagagcca aaagaaagtg tcatattcat
    20581 cttagcaacg accgaacctc acaaaatacc agctacgatc atttcacgga ctcagcgatt
    20641 tgattttaaa cggatcaata cggcagatat tgtcgaacat cttgaattta tcttgaaaga
    20701 atcgactatc agctatgaac cagctgcatt gaatgtgatt gcccgttctg cagaaggcgg
    20761 gatgcgggat gcattgagta ttcttgatca ggcaatctcg tttagtgaag gaacaattac
    20821 tttagatgat gccatgcaag tgacaggtag cttaactgac gaaatgatgg acagttactt
    20881 gagtgcttgc gtgaaaaaag aagtaccaga agcattggaa acattgaacc aaattctcgc
    20941 agccggcaaa gaatcacgtc gctttctaga ggatatgtta caatactgcc gtgacttgct
    21001 gatgtatcag caagcaccta acctagttga ggaacaatcg agtacgatca gaagtacatt
    21061 tacagaattg gcacaaactg tttcagcgga tcagctgttt tcaatgataa agattttgag
    21121 cgatactcaa aatgaaattc gttttaccaa tagtgccgct atttatttgg aagtcgctac
    21181 agtcaaactt gcacagctac cgtctgcttc tgttccttca gcagatgtag catttgcaga
    21241 tgatcagata gtaaaacagc tacaacaaga gattaaggaa ctaagaaacg aaatcaagga
    21301 actacgcagt aatacaggaa ttgagaatac aaaagtacag ccggtacagc aaacaaatca
    21361 taaaaaacag acaagtacct atcgtgtacc gacggaacga gtatataaag ttctgcggga
    21421 agcgacaaga cagcatctga accaagtaaa agaagtatgg gaagacttgc tgatgagtct
    21481 atctgtcact cagcgagcaa tgcttaaagc aagcgaacca gttgcagctg gacctacagg
    21541 actagtgatt gcgtttgatt atgagattgt ttgtcaaaga gcagcaaatg acgaagaatt
    21601 gcagctttcg gtacacaaca acttaagccg aatgatcaaa gattatgcgc ctgatcctgt
    21661 ttatattaca agagaaagct ggccagaact tcgaagaaat tatttgacgc aggcacaaga
    21721 agagggaaca gaaaactctt ttgcttctga agactcagaa ggagaaatcg agttgcttcc
    21781 tcaagaacaa gaagtaccag aagaaacact tgtagtaaaa gcagaggaac tgtttggctc
    21841 cgaagctgtt acagtggtag atgattaata aaaaggagta gataattatg atgcgtggaa
    21901 tgggcaatat gcaaggcatg atgaaacaag tacaaaaaat gcaaaaagaa atggcagaag
    21961 cacaagagct gctaaataaa acagagttta caggtaaagc caccaatgaa ttggtcaaag
    22021 cggtttttac aggcgatcgt cgcatgaaag atatccaaat caatgaagaa gtcgttgatc
    22081 ctgatgatat cgagatgctt caggatcttg tcgtaatggc agtcaatgat gcgatcgcac
    22141 aaattgatgt ggaaactgag aaaaaattag ggaaatacgc aaaaggtttg ccaggatttt
    22201 agttttaaac tgaaggagta atgaatatgc aatatccaga accaattgcg aaactgatcg
    22261 acagctacat gaaacttcca ggaatcggtc agaaaacagc gacaagactc gccttttata
    22321 cgatcgatat gaaagatgaa gtagtaaatg aatttgctaa atctttgtta agtgtaaaac
    22381 gcgatttgca tttttgtagt atctgcggaa acatcacaga agaagatccc tgcgaaatat
    22441 gtagaaatcc ttcacgtgat cgaagcgtta tcctagtggt cgaagagccg aaagatgtaa
    22501 tggcaatgga aaaaatgcga gaatatcacg gactttatca tgttctccat ggtgtgttat
    22561 caccgatgga agggacaggt ccagaagaca tcaatatttc ttctctgttg cagcggctgc
    22621 atgatgcaga tgtaaaagaa gtgattatcg caacaaatgc aacaacagag ggagaagcaa
    22681 cagctatgta tctctcacgt ctgatcaaac ccgcaggtat caaagtgact cgtctagcac
    22741 atggtctgtc tgtaggctct gatatcgagt atgcagatga agttacttta ttgaaagctg
    22801 tcgaaggaag acgtgagtta taaatttcta attaagtgtt ttttcagata caatataaaa
    22861 gcagcacaag agatgggggc aacaatgtga acggactatt tattacaatc gaggggcctg
    22921 atggtgcagg aaaaaccagc atcttgaatg aactttaccc tcttttgaaa gaagtagcaa
    22981 aaacagaaat cattcaaaca agagaaccag gaggcattcc gattgctgaa gagatccgag
    23041 cagtgatcct tgatccaaaa aatgatcgga tggatgaacg tacggaagca ctgctttatg
    23101 cagcagcaag aagacaacat ctagtcgaaa aggttctgcc tgctctggca aaagggaaaa
    23161 tcgttttatg cgatagattt gtcgacagtt ctcttgctta tcaaggtgca ggccgacgca
    23221 tcggcgttac cgagattgct cggttgaatg agtttgctac agaaggtact acgccggatt
    23281 ttacccttta tcttgacgta gattcagaca ccggattaag aagaatcaag aaaaacagac
    23341 aaaatcaaat cgaccgttta gattcggaag gtttggaatt ccaccagcgt gtacgtcatg
    23401 cttacttaaa gctggcagaa gagaatccgg aaagaatcca caaagttgat gcaagaaaaa
    23461 gctttgaaga agtgctgcaa acaagttatc atacaattat tgaacagtat ccgcaatttt
    23521 ttgaaaacta ggaaggtgaa cgtttatgaa aattatttta gcgattatcc aagacaaaga
    23581 cagcaaccgt ttatcaaatg aattgattga tgcaaatatc cgtgccacaa aactatcatc
    23641 gacaggcggc ttccttaagg ctggaaacag tacgtttatc gtgggtattg aagatgaccg
    23701 tgtagaagaa gcattggaaa tcatcaaaaa aacgtgtgaa tcaagaaagc aatttgtatc
    23761 tacacctgtt acactggata tttcgatgga tgggggcgtc ccttatccag tagaagtaga
    23821 agtaggcggt gccacagtct tcgtcttacc tgttgaagga ttccatcaat tttaggagga
    23881 gctgagctat gaatcaagaa ataaacttgg ctcaaatgca accgatcgtg tatcagcagc
    23941 ttcaacggag ttttgagcat gggcgtatcg ctcatgctta tctttttgaa ggggagaaag
    24001 gaacaggaaa acatgaagta ggtatatggc ttgcacagca cctgttctgt acccaaatga
    24061 aagacaatct gccttgtgga aaatgcaaca attgtcagcg gatcgaaaag aaagaacatc
    24121 cagatgtttt ggttattgaa ccggaaggac aaaccatcaa agtcgatcag atcagacgtt
    24181 tgcagacgga attcagtcgc agcggttacg aatcaagaaa aaaagttttt ttgatcaaag
    24241 aagctgaaaa aatgaatagc agtgcggcaa acagtttgtt gaaattcttg gaagagcccc
    24301 ctggagattt tttagcggtg ttagaaacag atgcagtagg aagaattctc cctactattc
    24361 aatctcgttg tcaagttctg cattttcaag aactttcgaa agaagcactg atccatcgat
    24421 tgcaagaaga gcagattcca ttggaaaaag caaaactttt ggctttctta acaaacagtt
    24481 taggaaaagc agttgaaatc tctcaagatg aatggtttaa tgatgctaag gacttgattt
    24541 atcaatggtt cgtttacttg caaaagcaag atactcaagc ctttatctat gtccaaaaaa
    24601 aattggtaaa atcatttaaa gaaaaaacac aacagtttac aggattatct gtcttgatgt
    24661 tttattatca ggaagcacag cgtcaagcag tagcaaacgg cgaatatggc aaagtgaaac
    24721 agataaacca gataattgaa cggatattga tcgcagagca gaaactacgc agcaatgtca
    24781 gttttcaggc tgttgcggaa cagttcgttt tgcatacgat tttttagctt gccgcaaagg
    24841 atgatgatca aggaggatgg aaatggtaga agtagtaggt gtccgatata gagatgcagg
    24901 tcatatctat tattttgcac caggagactc tgaatatgtt tatagtgaca aggtattagt
    24961 cgaatctcag caatccttac atattgctac tgttgtcctt cctaaaaaag agattgatcc
    25021 caatgacctt cctgaagcta tccgtccgat cttgcataaa gcgtcacaag ctgaattaga
    25081 aaaagacaaa aaaaacaaaa aagatgctta tgatgcattg aaaatcgcaa aagaaaaagt
    25141 gcagttgcgt gagttggaaa tgaaaatggt caaagccgaa tacacatttg atcgaaataa
    25201 attgattttt tctttcacag cggacgggcg aattgacttt agagaattgg taaaagatct
    25261 ggccgctgta tttcgtactc gtattgagct tcgacaaatt ggtgtccgcg atgaagcaaa
    25321 gataataggc ggaattggtc catgtgggcg tccattatgc tgttcttctt ttttaggcga
    25381 tttcgtgcct gtatcaatca aaatggcaaa agatcaaagt ttatctttga atccaacaaa
    25441 aatttccggt ttatgcggac gcttgatgtg ctgtctgcaa tatgagagcg aagaatacga
    25501 gacagcgaaa aaagaactcc ctgactttgg aaaagtaatt gctacacctg atggtaaagg
    25561 gaaagtcgta gggttgaatc tgcttagccg tatcgttagt attcggctga ctggacggga
    25621 aaatcctgtc gaatatgaat gggatgaatt gaaagaattt atccaagtag gtgagatgaa
    25681 tggataagag atcattatac gacggattga atcaattaga atcagagttg agaaatacat
    25741 taacagaact tgttgaaatg aagcatgctt tgcatgagat cgtagaaaaa aatacaactt
    25801 tggagatgga aaatcaacgt ctgcgggaac acctgcggga attgaaccaa gttgcacaag
    25861 ctccagctga aaatgtgaag caggaacttt ctaagtctcg tatgaatctt gaaaaaattt
    25921 atgaagaagg attccatgtc tgctacgatt tatatggatc aagaagggac aatgacgagc
    25981 cttgtgcgtt ttgtctagaa gtgatctatc gcgaaagcgg tcgatgacca ataacaagag
    26041 gagactggag cggagaaata ctgctccagt ctttttatta aggagattgt gacagatgta
    26101 taatcaaaaa agtttcaagg gaaatccggt atatggggat ttatatcttg taccgacgcc
    26161 aattggaaat ttagaagata tgagctacag aagtgttcgt atgctgcaag aatccgatct
    26221 tatcgcaagt gaggatacgc gtaatacaca aaagttattg aatcattttg aaatccatac
    26281 accccaaaaa agtttacatg aacataatta taaagaacgt gtccctcaat tgattgcttt
    26341 gttaaaagaa ggaaaaacga ttgctcaagt cagtgacgca ggaatgcctt ccatcagcga
    26401 tcctggacat gagctagttt tagcctgcat aaaagaaggg atttctgtga tctctatccc
    26461 aggacctaca gctgggatga cggctttgat tgcttcaggg ctagtgcctc aaccattttt
    26521 attttatggc ttccttccac gtaagaaaaa agaacagaaa gaaacattga aaaagctaaa
    26581 agaacagaca gttacgctga ttttttatga gtcaccttat cggatcactg ctacattgac
    26641 gaatttttta gaaatttttg gaaatcgcca agtcgttttg tgtcgggaat tgacaaaggt
    26701 ccatgaagaa taccttagag gaagtacgga agagctactc gcttatatcg aagaacatcc
    26761 agtaaagggt gaatgttgtc tgcttgtaga agggaatttg ttcgaaatgc cagtgacgga
    26821 agttgatgaa gagttgggaa cgttaaagga acaagtagaa gcaaagatcg cttcaggcga
    26881 aaaaccaaat gctgctatca aagcagtagc gctgaaaaat gggctgaaaa aacaagaagt
    26941 ctaccgtcag taccatgaat tagactgaga tagtataagt tgtctataat gaaagactaa
    27001 tgaggaaatc agctactaac ttttcaaatc tcttgaacgc gaacgaatgt tcttgtataa
    27061 tgaataagag gtgaggatga tggagctgca atttccttta cataatgata catcaagaaa
    27121 aatcattcat attgatatgg atgctttttt cgcttcggtt gaagaacgag atcatcctga
    27181 gtttcgcgga catccattag taattgccag acaccccagt gacactggtg ggcgtggagt
    27241 agtgactaca gcaaattatg aagcaagaaa attcggaatt cattcagcaa tgagtgcaca
    27301 aaaagcgtat gagctttgtc cgcaagccat ctttaagcct ggcgaccatc ataaatacag
    27361 cgatatttcc aaacaagtac gggaagtatt ctatcgttat acagatattg tggaaccagt
    27421 tagtatcgat gaagcgtatc ttgatgtgac cgaaaataaa atcaattgca agtcagccat
    27481 caaaatcgcc cgaatgatcc aagcagatat ttgggagagt gtccagctaa catgttctgc
    27541 gggagtaagt tacaataagt ttttggccaa attagcatca gactatcaaa aacctagagg
    27601 aatcacggta gttgcccctc aagacgctgt gccattttta aaagctttgc caattgaaaa
    27661 atttcatggg gtagggaaga aaactgtacc tagaatgcat gagctaggga tttatacagg
    27721 aaaagaccta tatgaatgca cagaaatgat gttgattcga aattttggaa aaatgggcta
    27781 ttctctttat cgtaaagtca gaggaatcca tgattctcca gtaaacgtta caagagagcg
    27841 aaaatctgtg ggaaaagagc atacttatgg gcagccgttg caaacggaag aagcagtgct
    27901 gactcagttg cgccaactag ctgaaaaagt agaagaagca ctgagaagag tccaaaaaca
    27961 tgggaaaaca gtagttttga aggtcaggta cacggactat tctactgtga ccaaaagagt
    28021 gacattgcca gaatatatct ataaaaaaga agcactcttt tatcaagcaa gccttatatg
    28081 ggaggaaatc ttgggagtag aaaaaggaat ccgattgtta gggattactt tgacgaatct
    28141 ggacccgatg acttatgaaa acatcgtcct tccattatgg gaaaatcaag aaatataaaa
    28201 acagctttga gagttgaaac cggaaatccg ttttctctcc caaagctgtt tttatatcaa
    28261 gtcttgcttt ttgatttgtt cataggtttc tgttgcatcc gtacattttt cccaaatcac
    28321 aacttgattg gattctaaag atttttggac gtcaatgatc cgctggttgc tacttccgcg
    28381 aaattgtagt tttaggtcac gtttgcttaa ttcaaagcgt ccgtcaacaa ggatatcgat
    28441 ttgcgaaagg agttctaatt tatcttctga atcttgtagc aattcatcaa aggcatagcc
    28501 tgtccatgac cagatatctt ttgtcgtacc gaactctttt cggatacgtc gtacgacttg
    28561 cagacaaaca tctgtattca aaaaaggttc gccaccaagt aaagttaatc cttgcacgta
    28621 atcgttgcgc aagtcagcga tgatttgttc ttctaattca ggtgtgaatg gtttgccata
    28681 tcggaaattt tgtgcagctt tgttgaagca gccttcgcag gcaaataagc agccactaac
    28741 gtaaagactg ttgcggacac cttcaccatc gacaaaatta aacggtttgt agtcagcaat
    28801 atagttttgg ctgtagtctt ttgctaacca ttcttttggt tttggatttc tcataaaaaa
    28861 acttctttca tttaaggttc aaagatatag ctattgaaga taaaatctac tcgaccacat
    28921 aaataagaac aaactgacct aaatccattg ttcaagaaag cttgttctta ttttgataga
    28981 gggcgttcaa agatacaaag ccatcgaaag agggtggtga aaaaagactt gtcatagcct
    29041 cttattctga ataaacggtg ttcaaaagct tgcatctgca gtaataagcc caactaaacg
    29101 aaaaatatga ggaactattt ttgtttagct attcttatta cttgatgcag gacagctttt
    29161 tcacaacctc atttcatgtg tttcacacgt gaggaaattt ctttatggcg gccatgtacc
    29221 atcggacgag cttgcggatt tcctaaatag ccacaagtac gtttgactac atcacatgtt
    29281 tttggatcat gatttccaca ttctggacat tcgaaccctc gttctgtcgg tttgaaatca
    29341 ccttcgaaac cacattgata gcaatgatcg attggcgtat tcgtaccaag atacccgatt
    29401 ttgtcatatg caaaatccca tacagcttct aaagctttcg gattttgttg gagcataggg
    29461 tattcacagt agtggatgaa tccacctgaa caatattttg gataatcctt ctcaaattcc
    29521 aatttttcaa atggcgttgg gtttttacgc acatcatagt ggaaactgtt tgtataatat
    29581 tctttatctg tgatattttc gatgatgccg aatttttctg tatccaaacg gcagaaacgg
    29641 tcagttagac tttcacttgg agttgaatag acactgaaat gataaccata ttcatccccc
    29701 catgcatctg cattagcttt taattctttg acgatatcaa gtgtgaaagc ttttgcttct
    29761 gggtttgttt cccattcgcc accgaagaaa gctgaagcaa cttcatacaa tccaatgtag
    29821 cctaacgata cagtagctcg acggtttttg aataattcat tgacagaatc ggtacgtgaa
    29881 agacgtttcc cgaaagaacc atacatatat aaaattggcg catttgctgg aatggcttct
    29941 ttacaacgtt ctacacgata gagaagcgca tctttcatga tttctaagcg gtcagacagt
    30001 aactgccaga atttattctg atctcctttt gattcgatcg cgattcttgg caaattcaat
    30061 gtaacaacac ctaaattcat tcgtccgaca tttacttctt gaccattttc gtctttccac
    30121 ccttgcaaga atgaacgaca gcccataggt actttgaaac ttccagttaa ttcaacgatt
    30181 ttatcgtaat tcagaatatc aggatacatt cttttcgttg cacattccaa tgctagttgt
    30241 ttgatgtcgt aattaggatc ttcagggttt aagttcacac cttttttcaa agtgaagatc
    30301 agtttaggga agatagctgt ccgcttttca ctccctaatc cattgattcg aatctgtaaa
    30361 attgcttttt gaatttctct ttcaaaccag ttttctccta gtccaaatcc taatgaagtg
    30421 aaaggagttt gtccatttga agtaaataag gtattgattt catattccaa actttgcata
    30481 gcatcgaaga tatctttttt ggtttttgct tttgcatatt ctttttggcg ttctggcgaa
    30541 tcgatccatt cttccgcgtc ttttagatgt tttttatagt tcaattctgc aaaaggagca
    30601 agcaattcat ctgttcggtc ggcagaacag ccaccatatt gacttgacgc tacattggca
    30661 ataatctgtg agatttgtgc tgtggcggtc tggatagatt ttggcgattc gacttcggca
    30721 ttgccgattt tgaacccatt gttcagcatg cctttgaaat caatcaagca gcaattcgtc
    30781 atcggtgtat aaggatgata atccaaatca tggtaatgga tatcgccttt ttgatgcgca
    30841 tttgctatat gtggtggcaa cattttcagt ccgattgatt ttccaacgat cccggctgtc
    30901 agatctcgct gtgtattgaa aacattactg tctttatttg cgttttcgtt cacgacagtt
    30961 tggtccttgt tgattagctt ttcaatcgaa aagttgatat ctgttgcttt actgcgagca
    31021 aaatctcgtt tggtacgata attgatgtac tcttctgcta attcatattc gtttttttct
    31081 agtaatatat gttccacgat gttttgaatc tcatagattt tgacattttc agcaaaccgg
    31141 cttttgattt cttggacaac caagtctgca atcatttgaa ttttttcata agtaagtggt
    31201 gataatgagc cgtgaatttt cttttctgct ttgatcagtg cttcattgat cttttgttca
    31261 tcgaaatcaa catggcggcc atcgcgtttg atgactttca tctgggatgt ctctggctgc
    31321 tgagtaacga cagttttttc ttcataattt actttcatta tcttcaactc ctgctaaatt
    31381 gttcttgtct atcataaaac aaaatcgggg aaggagcaac taaatatcgg ctatatatag
    31441 tattcaatgt gctgtataat tggatttcta tactatatgt agtgtatatt ttattgtgaa
    31501 aaaaggtgaa aacttgtagc tggtggattt gttttttgca tatttaattt tgctgaataa
    31561 aataattgaa taaaattttt tattcagtcc aagtaaaagt gacaaaaaca taagagcaaa
    31621 ttattcgtag atatgctact atgcaaataa atagaaatga aaaatgaaag aggaggagaa
    31681 gaattggaaa agatgatcga agtgaaacat ctaagtaaaa gctacggcag caaactttct
    31741 cccgtcaaag ccttagataa tatctcattc acagtagaag aaggcgagtt tgttgggatt
    31801 atgggcccaa gtggtgcagg aaagacaacg ctgatgaata tcttgtccac gatcaatttg
    31861 cctacgatgg gcagtgtctc gatccaagga aaagaaatca caaaaatgaa aaacagtgag
    31921 ctcagtgact ttcgtcgaaa aaaactagga ttcatttttc aagagttcaa tttgatcgat
    31981 accctaaatg caaaagacaa tatcctattg cccctagcag ttgaacggat gactaaagag
    32041 gaaatggaaa agagagtaag acatgtagct caattgttga atatagaaga actgctcaaa
    32101 cggtatcccg atgaactttc tgtcggacag agacaacgaa ttgcagctgc tagagcactt
    32161 gttgttcagc cacaagtgat ttttgcagat gaaccaactg gctctttgga ctcgaagtcg
    32221 gctacagaat tgttgaacta cttgaagacc atgaatcaaa aagaaaaagc aacgatactg
    32281 ttagtcaccc atgatccata tacagcaagc tactgcgata gaattttatt tattaaagat
    32341 ggtgtgattt tttctgaagt tgtcagaaga gggacaagaa gagagttctt tgagaaagta
    32401 atagacatgc aggcgacgat tggcggagga ggaaaaatga atgctgtata aactagccgt
    32461 taaaagtttt tttcggcagt cacgaggcta tcttgtttat tttttcagtt tgactttatc
    32521 cgtaatgatc tattattcct tcagcgcaat gacctacgat caatctttag tcagaagagc
    32581 aagtcaagat acttcgatag atggtggatt gagttttggt ggatttatca ttactgttgt
    32641 tttgcttttt ttcgtattca gtgccaaccg atttttcttg aatagaaggc aaaaggaaat
    32701 aggcatctat cagctttttg gtatgaataa actgcagata tcaggtatat atgtgttaga
    32761 aatcatgata attggattgt tcgcctgtat ttctgggata ttactcggta ttattttttc
    32821 caaactcttt tcaatgattt tggttcgaat gatggatatg gatcttacta gtccattttt
    32881 tatctccatt ccatcagtag tagatacttt atttgctttt ttcatcatcc ttttagcagt
    32941 atctgtatat tcaatatgga aaatatggcg ctatccaatg attcgaagtt ttggagaaaa
    33001 agaacaagca gatagttcaa caatgcgctt caggacaagg caccgattac tcgcagtcat
    33061 cgggttacta cttttaacga gcggatattt tggagctagt cattttaggg aaatcacttc
    33121 atggatgatc agttatcatt atcttgatac attagttttc ttttttcctt ttttaatttt
    33181 tttcgtttgt gtggtcggta cttatttgtt tttctgttat acatttcggt tatttttgat
    33241 catattgagt aaatggaaga tttattacta tggtatcaat tttttgataa tgggtaacac
    33301 gcaagttcat ttgctcaaaa gttggcgtac aaattctctg attacagtca ttctcggaat
    33361 ttctttaatg acaattggcg gcacggctag tatctctact attttatcct accaggagaa
    33421 agtttcctcc ccgatggact atcagtttga tgttgagaca gagaaaaaaa tcaagccgat
    33481 tctagcagag gaagggcaga agataactaa agaaatcacc ttgcattata aagtcattgg
    33541 gagtatctat agtcaaaatc tcgaagaacc tatgtttcaa actgctaatc tgatcacgga
    33601 aacacaatat caagccttca gaaaagttaa tccagatttg ccgaacattt ctttgaaatc
    33661 acctggtcat actgttttgc ttgatcaaat gcaaactctt tttcggaata tttcattata
    33721 tggatctagt attggactgc cagataatca agaattgaag attcaacaaa tgcttccaag
    33781 tgtattaggt gacgaaacaa tggtatatct aagtcctgta ctgattgtag atgataccgt
    33841 ttttgaaaaa gcaaaaggtg tggattatca agtagtcaat gtagatgtat caggtggaaa
    33901 taatgaaaaa atagatgaac gactgagcaa tgacctgtcg ataaagtggc tcaatgctgt
    33961 ttattattct tatgatatcg taaatggaag tctacaagga gaaattagca caaagaaact
    34021 tccggatgac caaatgaagg aagcacaatt tagccaagaa agctctcgtt tgaatttctc
    34081 ttccagctat tcgaaattgc gtgcagttaa tcggcgaatc ggtatcaata tttttgtcac
    34141 actatttgtc gggatgatcg tgattattgc tactggtagt attttgacag tccggcaatt
    34201 agcagaagca gaggaagaaa aagaaaatta tcacctattg actaagctgg ggattccaca
    34261 aagaaggatc agacggatga tcttcgagca aaacgcactt accttctttc cacccatgat
    34321 attagggatc actcacgcag tatttgcaat caatgtcttc actcagtata taaaaactgc
    34381 cgattactgg ttggcttatc ttgtgagtgg cttattgatc cttatctatc ttttgcttta
    34441 tgtcattacg agtcagttat attgtcggat tatcgaggaa tgatatacgg aataagacaa
    34501 gataataaat agaactaaat tttgaatacc atttatttgg aataagaggt tgtgacacga
    34561 tttttgtcac aacctcttta ttttttatat tttttaaaag tacgagaaat tataaagaaa
    34621 aaagcagtga tttgatgtgc tatttttata aaagctatgt gacaaaactg ttagtaaaaa
    34681 aacggtttct gtaagctaga cacatgtttc tttttgtcaa aattagttac actttgtgtg
    34741 aggttatatg aggagggaat ccaatgaaca aatggcgttc taatccaaaa ttgaattatt
    34801 ggggaaaatt catcattcga acaatttttt atagtgcagt actgctgttc ctgatttatc
    34861 tgtatcacta taaaaatatt caaggaggaa cttttatcta taacgaattt taggaggaag
    34921 agtatggaaa tcaaaacgat tattgaagca atcgatgatt ggggaataaa tgaacctgat
    34981 cgagttgctt atcaggcgga tgagacgcat acttttggtg aattgaaaca agcatcagac
    35041 gcacttgctt attatttaga aaaaaaggta gaaggcgatg gaccaattgt cgtttttggc
    35101 aatttggagt ttgaaatgat tgtctcattt ctaggggttg tcaaatctgg ccatgcctat
    35161 ttgccgattg aagagcatac acccaaggag cgtatcctat cgatctttcg ggtggctaaa
    35221 ccatctatgg tcatcagtat cggggactgg atagaagata ttccttcggt tcctgtcata
    35281 acaaaaaaag aatttcaaga aatcaagaag ataccagtag gattttatcc cagaaatagc
    35341 gtaaaaggta cagaaaattt ttatattatt tttacttctg gtactactgg agaaccaaaa
    35401 ggcgtacaaa tcagtcatga taatctagtc agctttgttc aatggacgat ggaagacttt
    35461 gggatcaagc ctggtatgca tttcttagcc caagcgccat tttcttttga tttatcggta
    35521 ttttctattt atcctgcatt agtttctggc ggaatgctga agccgttaaa taaagcagtt
    35581 gttcaagact ttcgtcaatt attcgcgaca ttgccagcct tgaagttgaa tgtgtgggtt
    35641 tctactccat catttatgga tatttgcttg atggaaccta cattcaatgg agaaaatgtg
    35701 ccggaattgg acatgttttt attttgcgga gaagaattga cgaaaaaaac tgcagaaagt
    35761 ctacttgatc gattcccaaa tgcacggata tataatactt acggtccaac agaagcaact
    35821 gtagctattt caagtattca gattgaccag catgtactag atagttatga ccgactaccg
    35881 attggctacg taaaaaaaga cacgaaagtt tctattgtcc aagaaggtaa acctgtttca
    35941 aaaggagaaa cgggtgaaat cattattgca ggaccaagcg tatcaaaagg ttacctgaac
    36001 aatcccgaaa agacagctgc tgcgttcttt tgtcaaaatg acttagcaag ttaccataca
    36061 ggggatgcag gtagactaga tgaatatggg cttttatttt atgaaggacg tatggatcag
    36121 caggtcaaat tacatggata ccggattgaa ctcggggata tcgaacatta tctgctccaa
    36181 gacaaccgaa tcaaacaagc tgtcgtcgta ccaaaatacc aaggaacaaa agtacagcag
    36241 ctggttgctt ttgtcgtatt agaaaacaaa acgaaacagc cggattttca gttgactaaa
    36301 tctatcaaag aacaattact agaagttgta atggattata tgattcctca gaagttccat
    36361 tatgtcgatc tgctgcctca gacagttaat ggaaaaatcg atcgaaaaaa attaacagct
    36421 gaggtgaatc ctgaatgatg ttacctcata tgatccctta tgcagagccg ttttattttt
    36481 ttcttttagc cattgcgctt gtaccgatcg ttctatcttt attgatatgg gaaaaaagat
    36541 taccagttta tcaatcagca gtgacacttt tctttttatt tgtcagcttt ggaggtaatt
    36601 attggcaaca gggagcggcg ctgattgggt atgttttgtg gcagacgata cttgtatggg
    36661 gctattttgt ttatcggcaa aggaaaaatc agacaggatg gttttttgcc gccgttattt
    36721 tagcgataat tccactgttt ttaacgaaag tgactccttt ttttgaacat ggaaaagcat
    36781 cgttacttgg ttttttagga atctcttatt taacctttaa atctgtacaa atgatcatgg
    36841 aaataagaga tggcatgatc aaaacatata agccatttcg ttatatccag tttctgctgt
    36901 tctttccaac aatctcttct ggaccaatcg atcgctatag gagatttgaa aaagatcagc
    36961 tccatccgcc tgttcgtgat aaatatgttg atcttttggg aaaaggcgtc caaaatattt
    37021 ttgtcggttt tttatataaa ttcattatcg gttattattt tggtcaagta ctgcttcctg
    37081 tagtgggacg cattgcctta cagcatggtg gtatttcatg ggcattagta ggttatatgt
    37141 acgtgtacag tatgtatttg ttttttgatt ttgctggcta tagcttattt gcagtcggta
    37201 caagctacat gatgggatat cagacaccaa tcaatttcaa taaacctttt ttatcatgga
    37261 atatcaaaga attctggaat cgttggcata tgacgctgtc cttttggttc cgtgattatg
    37321 tatatatgcg attgatgttc ttcatgatga agaaaaaatt ttttaaaagc aagatcgtaa
    37381 tgtcaaatat cggttatttt gctttgtttc ttctaatggg tatatggcat ggattgacgt
    37441 ggtactacat cgtatatgga ctgtatcatg cttgtctgat ctgtctgaca gatgcatgga
    37501 tccgatttaa gaagaagcat aaagagcagt tgccttctaa caaatttacg catggcttag
    37561 ccgtattttt gacatttcaa gcagtatgtt ttagcttcct gattttttca ggatttttag
    37621 ataaactttt ttttagctaa cgaatagtga catattaatt tatataacaa aggagaaaag
    37681 gaaaatggaa gaacaaattt tatcaatctt agaagaaata acaggaacag atgaagtaag
    37741 aaaaaatcgt gatattgatc tatttgatga agggttgctt gattctttag catctgttca
    37801 attattagtc gagctagacg gagagctgga tatccaagtc cctgtatctg aattcgagcg
    37861 agaggattgg agcacaccga ataaaatcat tcaacaagca actgcattga agggttgagt
    37921 atgaaaaaga aactttttgc tattttcggc ccggttgttt tagctgttgg cttactgttt
    37981 gtgtttttta cctcctccta tcaagtagat atctctaatc cagaaacgat tagacgtgcc
    38041 tcttcttcca tgtcagtgaa cgtgttgaaa ggagatgcga taaaaaatta tgcattatct
    38101 gaaaaacaat atattccttt ttttggctca agtgaattaa gcagaatcag tccatttcat
    38161 ccgtctgttc tggcagaaaa ataccagcga aactatcgtc catttttgct gggagcaccc
    38221 gggacacaat cgttaagcca atatatgatg atgcgttctg caggagatgc tatgaaaaac
    38281 aagaaagtcg tctttattat ttctcctcag tggttcgtta aaaatggtgt aaaaactgat
    38341 tactttaata cgtattattc tgaacttcaa acatacgact ggttgttttc aatgaagaaa
    38401 gtaacgcctg ctgatcgcta tttagctaga agattattaa ctttctcaaa agtaaaagag
    38461 aatgatacgt tgacggcgat tctgcaaacg atcaaaaaag ggaagctacc tttacctgaa
    38521 tctttgaatc agctgcgcag ccaatggaac atgctgaaga gagaggatga agtattcagc
    38581 aatattgggt tgaaagatcg acagcaaaaa atcgatcatg agtcaaaacg attaccaaaa
    38641 caataccaag aaacagaatt atccatttta gcaaatcaaa ttggagaacg agaaacgacg
    38701 aataatccat tcgggttgaa aaacgatttt tatacgcatc gtatccgagc ccacgagcca
    38761 gagctcaaac agtcacaaaa aaattgggat tatcgttttt cgccagaatt ttctgatttt
    38821 cagctggtct tggatcaatt agcaaaaaac cataacgaag tattgttcat catcccaccg
    38881 gttaacgaga aatggagcga ttataccgga ctatctcaag aaatgttaca aggatttgcc
    38941 aaaaagatca aatttcaatt aaatagccaa ggattcaatc gaatagctga ttttgtcaat
    39001 caagcgggta ccaactattt tatggaagac acgatccatc taggctggaa aggctggttg
    39061 gctgcagatc aacagatcag accgttttta gaagaaaatc atattactgc tagtaagtat
    39121 catcttgacg atgctttttt tagcaaatct tggcaacatc aaataccaga taaactacaa
    39181 ttaaaataaa gaaaacaggc gagtgttgct cgcttgtttt ctttattttt gaatattctg
    39241 aataaaagat aaaaatacca agtttgcctg aacaaaaatc ataagcaatg gtataatcac
    39301 aatatgaata tagaaatggg ggaaaacatt tggatacaaa agaagaacag ctacttattg
    39361 atttaacttc tgcaaaagga gtacctggaa atgaagaaga ggtaagagaa gtattcagag
    39421 aatatgccaa accattcgct gatgatattt tttatgacgg tttaggcagt gtgattgcca
    39481 aacatggtgc tggcggaggc cccaaggtct ttatttctgg acatatggac gaagttggtt
    39541 ttatggtaac aaaaataacc gaaaaaggat tcttggaatt ccaaactctt ggaggttggt
    39601 gggggcaagt catgcttgct cagcaagttg aaatcaaaac acgtgaaggg aaaatcgtcc
    39661 acggtgtgat tggttccaaa ccaccacatg ttttaacacc tcaagtaaga aataaacctt
    39721 atgaaataaa ggatatgttc attgatattg gcgcatcaag ccaagaagaa gccaaagagt
    39781 ggggcatacg tcctggagat atggtcactc catatattga atacaaacga atgaatggca
    39841 gtaaatattt actggcaaaa gcatgggata atcgtatcgg gacagctgtt tctctgcgag
    39901 tattagagaa tctatctaaa gaagcacacc caaatgtttt attcgcagga agcgatgtgc
    39961 aagaagaagt aggcttgcga ggtgctcgaa caagtacaca ccttgtaaat cccgatattg
    40021 cttttgcttt agatactggt acagcaggag acactccagg aatgacacca aaagaagcag
    40081 attcgatttt aggaaaaggc ccacagattt tgatttttga tgcatctatg atcccgcata
    40141 aaaaattatt gaattttgtt attgatgtag cagaggaaat ggatattcca ttccaatata
    40201 cagtgatttc tggcggagga actgatgcag gacaaatgca tttgacaaga gatggtgttc
    40261 cgtctgtagc cattaccgtt cctgttcgtt atctgcattc ccatacatca gtgattcatg
    40321 aagatgatta tttgaataca gtgaaactag tcacagaatt agtgaaacgg cttgatagtg
    40381 aaacagttga aaaaattcgg agttatgaat aatgaataat agaataccta tttctgaata
    40441 tcggaaaaaa gcacgagttt ctttggaaaa tcaatgggga atcaatactt ggcttgtttt
    40501 cttatctata atatttacga gcgtcatcca gaactttttt gacggtttca gtccttttcc
    40561 ggaagaaagc atgcagcagc acttattgag ttttctagtc gataatttac ttttattcgc
    40621 attcaactat gcattgtttt acattgcgct agtagttgta cggggtggca gaccaaaaag
    40681 taacctttta ttttcagttt tccaaagaaa gtattttggt tcgatacttg gcatcaattt
    40741 gatcaattca gtcatcaact ggctactcaa cagtttaatc tttctgccca tgtttttaat
    40801 cagcggagtg agtacttata tgcagctgct tttcagttca aatgcggtac ctacgggtgt
    40861 catgccagaa gattttcttg atatcagctt tgttttatcg atgataggga tgatttttgt
    40921 ttcagcactc atcatgagta ttgttggcgg tttattccaa tttgctgcat ggacaaagtt
    40981 cgactatcct gatttgtcgg ttattcaatg tttgaaatat ggctggtttt tattgaaaga
    41041 tagaattggt acgtatattt tgttgcagct gtcttttatc ggctggtatt tgctaggagc
    41101 aatcgctttg ttcatcggtc ttttgtgggt cattgtttat gtgaatgtgt cagttgcaga
    41161 attttatgaa caagcacgga ttgaaaaagg aggtccagac gcctatttta cttagcatgt
    41221 aagcagttta ttcatcaaaa aaataacttt tgtggtagga taaatctttg agggggaatg
    41281 tacgatgaaa ttaactgttt taggatgctt aggagcgtat ccttataaaa atcaagggac
    41341 aacaggttat ttgcttcaat ctgacgggtt caatttgttg atcgatgcag gtagtgctac
    41401 gctgatcaaa ttacaagaac accttgatcc acttgatcta gatgctgtga tccttagcca
    41461 ttatcatcat gatcatattg ctgaccttgg tgtattacag tattactggc aacttcatcc
    41521 taagagaaat ccggagacat tgctttcaat ttatggacac acaaaagatt ccattcactt
    41581 tgaagaattg acgttggaaa atgtctcaaa aggacatcct tattttgaag ctgaagaatt
    41641 aaaaattggt ccttttctga ttacttttat ggaaacaatc catccagtag tatgttacgc
    41701 catgcgtatc gtagaagaaa caacggggaa agtattcgta tttacaggtg attcaggtta
    41761 tttggaaagt ttcgctgatt ttgcgaaaga tgctgatttg tttctagcag atacctatct
    41821 ttttgaagga aatgaacatc atcatgccca tttcacttca aaagaagctg gagaacttgc
    41881 gaaatcagct aacgtaaaga aacttgtact tacacaccta cctcagtttg gtgatttaga
    41941 acaattgaaa aatgaagcgc aaaaggcagc aggcaaaggg attgatatag aacttgctgc
    42001 agttgacaaa gtgtttaaca tttaggagga agaacatgat ttttgtacca aatgaaaacc
    42061 acgacccaag aatcaattta gcaattgaac aatatttgct gcaagaaatg cctttagatg
    42121 aacctatttt gctcttttat atcaatgaac cttcaatcat catcggcaga aaccaaaata
    42181 cgcttgaaga aatcaatcga gattatgtgg aagaaaatgg cattcatgtt gtccgtcgct
    42241 taagtggagg cggagctgtc taccatgatt ttggaaacct gaactttagt tttatcatgc
    42301 ctgatgatgg ggattcattc cgtgactttg cgaaagtaac acagccaatc atccaagcac
    42361 ttcatgaatt gggtgttgct ggggcggaat tgaaaggccg aaatgatcta gtcattgatg
    42421 gcatgaaatt ttctggcaac gcgatgtatg ctaccaacgg tcggatgttt gctcatggaa
    42481 caattatgtt cgactctgat atcaatgaag tagtcaatgc attaaaagta aaaaaagaca
    42541 aaatcgaatc aaaaggaatc aaatcgatcc gttctcgtgt aacgaatatc aaaccatttc
    42601 ttccagaaga aaagcaaaaa atgacaacag aagaatttcg tcaagaaatc ttgttgaaaa
    42661 ttttcggcgt agagtcagta gaagaagtaa aaacgtacga attgactgat gaagattgga
    42721 aaaaaatcaa tcgaatctct gaggaatatt atcgaaattg ggattggaat tacgggaagt
    42781 ctccagattt caatttctct cgccaaaaac gtttttctat cggttcgatt gaagtccatt
    42841 tgaatgtctc agaaggaatg atcaaagaag caaaaatctt tggcgatttc ttcggactag
    42901 gagatatctc tgacgtggaa aaaaaattgg ttggacagaa atatgataaa gcatctctat
    42961 caaaaatagt cagccaaatt gatgtgaaga aatattttgg tgcgatagaa ccagaagaat
    43021 tgcttacctt actttattga aaataaaaaa tagcggaatc cacatatatg gattccgcta
    43081 ttttttatac aggattcggg taatttcgag agagttgaac agtgatccga tcaatgaaag
    43141 tatctttttc ctgcgttagt gttcctgtgt aacgatccgc tacgtctttc atcatcatta
    43201 agtcaaaacg ttcctgatca tctaattctt ctgtgatgcc tcgactaaat aggtttgtat
    43261 cgtaatgtgc actatgttcg caacaaatga cgaaatgttc ctcttgttcg tgtatcgaca
    43321 aataaatttt tcgatacagt ggattttcga tggcatgagt ttctctaaag gaatgctcta
    43381 aagcgtggat caaaagttga gccagatcgt catcgttgat aggaagttct tcgggaagat
    43441 ctgcttggaa ggttatctga atattccgtt tggcagctaa cttctggtaa cgcgctaaga
    43501 taagatttgt cacctggtgg ttgcaaaaag aacacagcat ttcgaaattt tttgcttctt
    43561 ctaatatctt ttccacatag tttttagctt ttgtttggtc cgtatgcatt aaatcttgca
    43621 gattctccat ctcttggaca aattccaggc gaagtgtatt tacttcttgg atatgctgtc
    43681 gaagctgctc ataatgttgc tccaacaaat catttttgat ttttaagaaa tctacttgtc
    43741 tccgatcttt gtcggtattt aagatgtgat cgattagatt gtagctgata ataacgatta
    43801 gaatgataat atataaaatc gtagaccaat tgatcgttgt ttgatagata cctaatgccg
    43861 tttggatgta aatcaagcaa tggataatgg ctaccaagac gatccaaggt gtacaagcta
    43921 cataaaaacg gttattctta tatgcttcgc caatgcatgc aagtgaagtg actaaagtgc
    43981 ttataacact agcaatatta gcaatcggca ttaattcagg aagttctgca ggagtaaaag
    44041 ttgcataaag cagaacgccg ataaaaatca aaagttggaa cgtacaaaaa attccgtacc
    44101 attttttata gtaaaacatt cggctccaat agtagcagat gatgaaagct gacgttaaga
    44161 taataaataa atgagataaa aaagaatgaa cgaatggttc aaaaagaata ccacttaaaa
    44221 tatcttctga tacagcttca aatcctaatg ccaaagcaaa gcaacttaag agaataagtg
    44281 agtattcaag acgtttttgt ttatacatca agtagatcgt atagaaaaag gtccctatag
    44341 cgataaaaac agcgattatc atcgtcaata cttgcggcac tgattgtgaa taaataaaac
    44401 taatcaatgg tccagttgtt cctagataaa ctttcggtgg cagtccagca tagttctgat
    44461 aaggagatga gactgtaagg gtcaattttt ttccgatata atttgctggt aaatctatca
    44521 cagccaatga gagtccggga ttgctttctt cctgataatc tttccgttgg tataaaatct
    44581 tttcgcctag atatacagta atccattgat ggtttccttt gattagtaaa tttgcttcgt
    44641 ccaacggttt ttcaagtgtc gtttccataa tgaatgtttc gtctttatcc acggtaggaa
    44701 tcgtacgagt gtacttgctg tcaaacttaa aatcaggatc atcttttgta tagaaaaccc
    44761 acgaatcttt tatttctttc atttgattgc tgtccgcagg agaaaaaata aaataaaaga
    44821 aataagcaat tcctgttaaa aataacaatg ataatataat gattatgctc cactttatac
    44881 gtaaataaaa agattctttt ttattatata tgttattaaa aaaagacata aaggtattcc
    44941 tttctaaaac ttgtaatgtt agggtaaacg ttttttaaac taacgtcaat tatttataat
    45001 aaaaaatgag ataaagatga ggaaatagaa ttagaaacat ataattatta aagggaaaca
    45061 tttttacagc tataatttga aaaaaattta tcgttattat atgattaaga aggattgaat
    45121 aaagaaagaa gttgaaaacg atacctgatt acaaaaaaat aacttttata agttctataa
    45181 cgttgattat atttttaatt cttattcttc tcgtcgtctt ggttccccga atttttggtt
    45241 tgactcctta tattattgag gacaaagaaa tgagtccata ttatgcacaa ggaaatctga
    45301 tatatgtacg accgaaaaat acaaatgaga ttttagtcgg agatattatt acatattatg
    45361 aaaattcagg aaaaaaaatc tcgacgcgtc gcgtaatagc cgttgaaaac aaccacgaag
    45421 atttttatac aaaagctgat acaaagactt atattgagcc tggagtagta aaaaagagaa
    45481 atattatcgg tagtcccatc tttcagattc catatattgg actagtcctt tcaaaaacgg
    45541 catttgcatg gattcagggg ttattcttta cgattgcttt ttgcctaaca ggaatcacag
    45601 catggaatac ctttgtagag ttgaaaaaga agcggagacg aacacagaaa tttttgtaag
    45661 aatgttaaaa acataaatgt attccagaga tccaacgact cttctgaaca tgaaacacga
    45721 gaaaacactc atgcagcttg tagatttagt cagttgttcg tataatgtgt gaagaggagg
    45781 agtagtatgc gcaatgaaat gatgcatcgt cctgtagtca aagaagaact attggatttt
    45841 atgcgtaccc agcaaaaaaa attaccgggt gagctaggca aactggaaga agaagctcat
    45901 gtagcagaag taccagttat tccccatgaa acagttgtat ttttgaaatt tttacttgga
    45961 cagcttcagc ctgaacgaat cttggagatc ggagcagcta tcggtttttc atcaagtgtc
    46021 atggccacga caatcggaga aaacgctcat gttacgacaa ttgatcgttt cgatgtcatg
    46081 atcgagaaag ctaaaaaaaa ctatgaacgt ctaggactga cagataaagt gactttgctt
    46141 gaaggtcaag cagcagatat cttgccggag ttgtctggtc cgtatgactt tatttttatg
    46201 gatagtgcaa aatcaaaata tattgaattt ttacctgaat gcttacgttt gttaagaaaa
    46261 ggtggcgttt tgatggtaga tgacatcttc caaggaggaa cgatcttgct cccagatgaa
    46321 gagattcccc gtggcaaacg agctatccat cgtaaattaa atgaatttct tcgtgttgtg
    46381 atggatcatc ccgatttgac atcgacaatc ttgccattag gtgatggtgt gatcttaatg
    46441 acaaaggaat cagaaacaat cgagttgtga tacatgatta tggcagcctg tagataatcg
    46501 attgaatcga gggctcaatt cgaacttgat gtaaaagttt ctatatttgt aagtcaatta
    46561 aaggatacag gaaatctaaa taaacaggag tagcatgaag tcaatgtagc tcctgttttc
    46621 aattgataac cttagaacag ctgaaggaga atgtctcgtt ttaaaagatt gatttagtag
    46681 ttgggccaaa ataattcatt atttgctaat taaataacta tttcgtattg acagaagcaa
    46741 tttaattatc tataattatt gatgttccgc tgattcttgt catagtagct cagctggata
    46801 gagcatccgc cttctaagcg gacggtcggg ggttcgaatc cctcctgtga cgctaaaaaa
    46861 ccgttagaaa ctgaggtttc taacggtttt tctttgtttt ttgtgccaat attgtttgtt
    46921 ttcaaaggca aataaaataa aaaaacatcc ttatcgagta caattataag gagcaaaaaa
    46981 ataaaactca tcagaaaaga ggaagaagat atgacgatac tatctagaat tttagtgact
    47041 ctagttgcaa ttgaattttt ttatatcatg tatatcgaaa cggtgagaac tgattctgac
    47101 acgacaagta gggtttttaa aatgagtaaa gaagagttaa gcagaaaatc tgttcaaaca
    47161 ttgtttaaga accaaggagt gtacaatgga ctattaggag tgggactact ttatggagct
    47221 tatctttctt ccgcatctaa agaaatcaca agtatgctat taatatctat tttttttgtt
    47281 gcattatatg gcagtttgac tagcgatcgt tggattttac tcaaacaagg tgggctagct
    47341 ctgttggcat tgctttctat tttcttataa atatagaaaa aataagaatt tatctaacag
    47401 gaaactttgt gaaaaatatt actttatctc atgaaatatg atattctaat gaatggtatt
    47461 ttagaaaatt tcatgaaaaa ggagttagat aggtgttcaa aaattatgtt tcctcactaa
    47521 aaaaagaatt ttcaggttat gatctttcaa gatttcagaa agatcttttg gctgggatca
    47581 cagtagctgc tgtagcattg cctttagcct tggcttttgg ggtatcaagc ggagctgatg
    47641 cggccgcagg tttgattacg gctatcattg ccggtatcgt tattggcggt ttatcaggcg
    47701 gtttttatca aatatcaggt ccgacaggtg cgatggcagc tatcttgatg tctattgcgg
    47761 caacaaaggg aatgcaaggt gtattgcttg ctactttttt agcgggtcta tttttacttg
    47821 cagcaggact tttccgatta ggaacactga cttcttttat cccagcaccc gtaattacgg
    47881 ggtttacttc aggtattgct atcattattg cattaggtca gatcgataat ttatttggtg
    47941 ttcactcgga aggtacaaac gttattgaaa aactaagtag ttatcaaacg ataggatttc
    48001 cgataaacac agcctctttg atcatgggag ggctagtcat tttagggatg tttatctatc
    48061 caaaaaaatg ggcgaaacga atgcctagtt cgctgctggc tatcattttg gcaacagcag
    48121 ttatgctatt gttccattta ccagtagcaa ctgtgggtaa gattccacaa acattgatca
    48181 gtagtaatcg tttgaatatg ggagatttta gcttttctgc cttgcaacaa gtggcagttc
    48241 cagctattag cattgcttta ttaggaatga ttgaaagctt gctatgtggt gcttcggctg
    48301 gccgaatggc aaataagccc ttagatagca atcaagaatt aatggcgcaa ggaattggaa
    48361 atctgttgct tcctttcttt ggcgggattc ctgctactgc ggccatcgcc cgtacaagcg
    48421 tggccatcaa atctggtgca caaactagat tagctggtat gatacatgca gttgtcttac
    48481 tgctctccat gcttgttttt gcgccagtga tgtcacagat tccaatgcca gcacttgcag
    48541 gtgttttgat tgtgacggct tggcgtatga atgaatggga aacgatcaag gagctattta
    48601 ctaataaata ttggtcagca gttctattgt ttatccttac gatgatttgt acagttatct
    48661 ttgatttaag tattgctatc gtgatcgggg tgatcggcgg ctgtgtattt tttgttgtca
    48721 aaagcgctgc tatcacgatt tcattagaaa cgatcgattg gcaaagaatg gggctgccag
    48781 aaacggaaaa gctgaataac tggacagtag tgtatattag tggtccactg ttttttatgt
    48841 cttcagaaaa actcaaaaat aaattggaag aactaaatca tcacgatggt gtcattttct
    48901 ccatgcgagg cgtaccaagt attgatttga cggcacgtgc agtttttgat gaatttcaag
    48961 aaaaagcaga gcaaagagga caaacgatta tctatacttc cttgcagcca gaagtagaaa
    49021 aacaactttc tcacttgtgg gaacatacat tcacgcatca acatcaaaca gttgctcatg
    49081 cgttgacagc tttacatgcg aaatatactg caattagcca ttaaaacaat gaaaaagaac
    49141 agacaagact gtaaactgaa gattttatgg tcttgtctgt tctttttaat attgctgtaa
    49201 aagtgaagct agtacttgcc agtcttgatg gataacaggt acaagggagc gattgtagaa
    49261 aatagctgct ggatgaaaaa gtggaagaat tcgatagttt ttttctgacc attcgtaggc
    49321 atccttttgt tctgttaatt gtaagacagg ccctgtatag agttgaccat gatttttggt
    49381 aaccgtatat tctttgccaa gtaaacgttg aagaccaatg tttccaagtg tcgcaattaa
    49441 ttttggctgg accttcatca gttcgtaatc caatattggt gcatgcgcaa atacttcgga
    49501 acgagtaggt gtccgatttg gataaacagt ttctgactgt tgtgtccgtt tattgatccg
    49561 atgagtcacg cgatacgggc ggctccttac agcactggtg atatacacct cttctcgtgt
    49621 taagccagtt gaagaaagtg cctgcatcaa ttcttttcca gcttgaccgc taaaaggaac
    49681 atgcgtatca atctctgttt tccctggggc ttctcccacg agcatcagtt taggatgaag
    49741 aggtccttgc ccagcgataa atccttctac aggacggccg ttcattctta aagaagcttc
    49801 ttgtatcagt tcttttgggt attccattta tgctcctcct ttttctttta ttataagaat
    49861 aaaagaaaca caatgcgact attttatttg gtagaactgt gaggatagaa aagcagaagt
    49921 attgcttgaa tttttgtttt actttttgat tttaaattta gttgactaac ggttgctgtt
    49981 ctggtaagct agtactgttg taattgtgga ctccacagct acaaccgctc agaacaagta
    50041 ataagcacag catatgctgt cacttcggat ggcgagtcta agtatatcag aaggaggtgc
    50101 tttatagcat gtacgcaatt attaaaactg gtggtaaaca agtaaaagtt gaagtgggac
    50161 aagcaatcta tgttgaaaaa cttgacgttg aagcaggaga aaaagttgtt tttgacgaag
    50221 ttatcttagt aggcggagaa tctacaaaag taggcgctcc aactgttgca ggtgcaactg
    50281 ttgaaggtac tgttgaaaaa cacggcaaac aaaagaaagt cgttactttc aaatacaaac
    50341 ctaaaaaaca ttcacaccgt aaacaaggtc atcgtcaacc atatacaaaa gtggttatcg
    50401 acgcgatcaa cgcttaatag cgtttgagaa agaaggccta tatatgatca aaggaacatt
    50461 taagcgaaat gacgcaggtc agatcgtttc attcactttg accgggcatg ctgatgctgg
    50521 tccttatgga agtgatatcg tttgtgcggg tgtttctgcg ttagcgatca gcactgtcaa
    50581 cgggattgcc tcattagccg gttttgagcc gattgttgaa atgaatgaag aagaaggcgg
    50641 ttatctttat accgaagtca cttcaggaat gacacaagaa caaaacaata ttgctgagat
    50701 tctattagaa aatcttttat taggattaca atcgatagaa gctgaaaact cagaatatat
    50761 ccaaattaaa acaattaatg ataaatagga ggtgcagacc atgttgttat ctatgaatct
    50821 acaattattt gcccacaaaa aaggtggcgg ttctacatct aacggccgtg actcagaatc
    50881 aaaacgtcta ggtgctaaac gtgctgatgg acaaacagtt acaggtggat caattcttta
    50941 ccgtcaacgc ggtacaaaaa tctatccagg tgtaaacgtg ggtatcggtg gagacgacac
    51001 tttatttgca aaagttgatg gcgtagtacg tttcgaacgt aaaggccgta acaaaaaaca
    51061 agtgtctgtt tatccagtag ctcaagaagc ttaattgaat cttatgatta aagaggttgt
    51121 gacaagactt ttgtcacagc cttttttgtt tataaaaata aaagttgtag tggaggctac
    51181 gaagtttact ttgtcttttt acacttaata tttttttgcc aatttggaat caggatgttt
    51241 tgataactat cgttttcaat tgatggaagc tatattgttt gctatcagtg aaataattat
    51301 atggtaaaca gtgttctttt tcttgtttcc attataaaaa tcgtatcggg atttttcgtt
    51361 cttttaaatt gaacatttct atctatccgc gtgttatcaa caaaacaaaa tcaggtaaat
    51421 agttgacgaa acagaaataa atacatagta ttatgttttg aagcctaagt acaatagtct
    51481 atagaaaagg agcgatacca ttggcaaaac gcattgaccg aatttatatg tatgtcaaag
    51541 aaaaaaccgc tcatttaaca aaggcagaat atgatcaagg agtaacaaca caggaaattg
    51601 ctgaagtgtt agggattcag cgaacaaata gcagtaaaga tctgaatcag ctagtgcgcg
    51661 aaggaaaact gataaagaca gacggacgac cagtacgtta catatatcaa acactcgtta
    51721 cgcacgtaaa gccagttaca aaacatgtag tcagttacaa agaaacgccg gtctctgaag
    51781 aaaaaaatac actccaaata gatactaagg atatctttgc aaaaatcatt ggagctaacg
    51841 gcagcatgaa aaattcagtg gaacaggcaa aagctgcgat tttgtatccg ccaagaggat
    51901 taaattgttt gattacagga cctaccggat caggaaagac ttattttgca cacgcgatgt
    51961 atcattttgc aaaagcgcat catgtaatag atgaggaaac ggaactgatt gttttcaatt
    52021 gtgctgatta tgccaataac cctgagcttt tgatgagtca tttatttggg tacgcaaaag
    52081 gtgcttttac aggcgcagat gaagaaaaaa tggggattat cgatcaagcg gatggcggta
    52141 tgctgttttt ggatgagatt caccggttgc cgcctgaagg tcaagaaatg atcttttact
    52201 ttatggatca tggaacttac agtcgtcttg gggaaacaac gaaatcccat gaagcaaatg
    52261 tacggatcgt aggtgcgacc acagaagacc caggttcttc tctattagaa acttttgttc
    52321 ggcgtatccc aatcaatatc aaacttccgg cttttgaaaa acgtcctgca aatgagaaag
    52381 tcgatttagt aaaaatcatg attgcccacg aagcaaaccg cacgcagcga aagatttctt
    52441 tgacagaaga tgtagcaaaa gcattgatcg gcagtgtgac ctatggaaat ataggacaat
    52501 tgaagtcaaa tatccagtta gtatgtgcac gaggatttct aaatcatatg caatcacccg
    52561 aaatctcgat cacaatcgat gatttgacag aagggattcg tagcggattg attcaattag
    52621 caagcaaccg cagtgcaatg gcagagcttt ctaaaatatt ggagccaaag ataactgtag
    52681 caccaaatga tacgatcatg aaaatccaat cggactccta tgagttacct tataatctat
    52741 atgatatcat cggagataaa gcagctttac tgaagtcgga tggtttggat caagaagcaa
    52801 tcaatcattt catttctaca gatatcaaca tccatctgaa gtctttctat aaagaccatg
    52861 gtttttcttt taatgcagac agcaaattag cagaatttgt agatccgaaa atcatagaag
    52921 tgaccaatca gatttatgta atggtcaaaa gatctttacc atatgagttt cagcaaaact
    52981 ttatttatgc tatgagcttg catatcagtt cttttttgaa aaaaatcaac ttaggagaag
    53041 aacggcatac gaatgataac atccgtgaga tggcttttga ttttccagat gaatatgctg
    53101 ttgcgaaaga agtgcgtagt tatatcgaaa cgtattttca agtggagatt ccagaaagtg
    53161 aagactatta cttgaccgtt ctactcgtct cgttacgggc aaatcaagca agcggcagaa
    53221 tcggtgtagt cgttgcagct cacggaaaca gcacagcaag cagtatggtc caagttgtcc
    53281 aacagttgtt agatgcagag aacgtacgag cagtagacat gcctttggat atggatccga
    53341 aaacagcatt ggaccgaatt gaacgcaatg tacaagaagt agatgaagga agcggggtca
    53401 tactcttagt agatatgggg tcccttgcgt cgtttaattc gcaaattcaa agagacactg
    53461 gaattccagt acgtactgtt gatatggtaa caacttctct agttttggaa actgtacgaa
    53521 aggtctcagt attagggaca gatctagata tgttgcatga atcattgaaa aattttcgag
    53581 gttacgcaga aatatcttct gtagaatctg ttcaggaagc aaaacctatt cataagaaaa
    53641 aagctgtttt ggctatctgc gcatctggcg aaggtacagc acaaagaatc aaagaattga
    53701 tcgaacgagc ggtaaacaag cgtcaagaaa cagaattaga agttctagct ttgtcgattg
    53761 tggaagtgaa agaagaactg ccagttatcc aaaaagaata tcagatcata gctactactg
    53821 ggattactga tttgaatatc caagccccat ttattccttt agaacgtttt attgatcaaa
    53881 atatcgaagt cattttggat cagttgctga tggaatcaga attggaggaa actgaattca
    53941 tctccctaga tgaagagtca gctaaaaata cgtgtgttga gtttatctct gataacttca
    54001 tctttattaa tggtagtaag ttaatcgatc caatgtggca attttctaca caaatctctc
    54061 agacgacggg aatcggcgat gaagaatatg gattcaaaat caacctagtg atgcacacag
    54121 caggaatgat cgaacgtatt attcggaatg aaccattgac tgtggaagaa aacgaattga
    54181 caaatacaac gaatgatcct ctttatagcc aattggcagc ctcagttgtt ctgttggaag
    54241 atcagataaa agtaaaagtg ccgatagaag aaatgtatta tctgctacgt ctagtccata
    54301 atcagttaga taaaaaagaa tacactgtgc catgaataca caaaaacgat acacttttat
    54361 agtgtatcgt ttttgtgtat tggttaagaa tcctttaaat tcaattagtg tatcgtaaaa
    54421 aagttggcac gctttttgct tatagtagtg tgtgctagga ggtggttgtt cacacagaaa
    54481 tgataggggt ttctgtgaag tagtgaggaa gaaggagttt tgcagatgga tattcgattg
    54541 gcacgtatag atgatcgttt aatccacggg caagtcgcaa cagtttggtc gaagcaaaca
    54601 ggtgttgaac gtatcattgt gatcagcgat gtagtcgctc aagataaatt gagaaagttt
    54661 ctactaaaag aagctgcacc gccggggatc acagcaaatg taataaccgt gcagaaaatg
    54721 attcaaatct atcatagtgg attattatca cgagcaaaag tgatgctttt attcaccaat
    54781 ccccaagatg ttgaagcggt tgtccgctgc ggtgtacagt tgaagtcgtt gaatatcggc
    54841 ggtatgagtt ttactgaagg caaatcaatg attaccaatt ttgtttcagt aaatcaacaa
    54901 gatattgatt cctttcgtta cttgaacagt caagaagtgg agttagagat tcgaaaagta
    54961 cctgctgaca ggaaaatcct tttgatggaa ctactagcga aaatcaaaaa ggtgtagcaa
    55021 gaatggttgt tggaaaaggt caaataattc tgttgtaatg gttagcaatt attaatattt
    55081 ttgaatttgt tacaacaaaa gtaaatagga ggtataaata tggtagggat tatcctagca
    55141 agtcatggac aatttgctga aggaatctta caatccggtt caatgatctt tggcgaacaa
    55201 gaaaacgtca aagccgtgat tttgaaacca agtgaaggtc cagatgactt gagagccaaa
    55261 ttggaagaag cggttgcttc ttttgataat caagacgaag tgttattctt agttgattta
    55321 tggggcggaa caccgttcaa tcaatcaaac acattattcg aagagcacaa agataaatgg
    55381 gcaatcgtca gcggtttgaa tttaccaatg ttgattgaag cctacgcatc acgcttttca
    55441 atggaatctg ctcatgaaat tgcagcacac atcattgaaa cagcaaaaga cggtgtaaaa
    55501 gtaaaaccgg aagaattaga accggcagaa gcaccgaaag cagcagttga agacgcacaa
    55561 cctaaaggag cacttccaga aggaactgtg gtcggagacg gaaaaatcaa atatgtatta
    55621 gcacgtgttg actcacgtct gttacatgga caagttgcga cagcttggac aaaagcagtg
    55681 caaccaaacc gtattatcgt tgtttcagat gcagtatcaa aggatgatct gcgtaagaga
    55741 ctaatcgaac aagctgctcc tccaggtgta aaagcaaatg ttattcctat tagcaaaatg
    55801 atcgaagtag caaaagaccc tcgttttgga aataccaaag cattgctttt atttgaaaat
    55861 cctgaagacg tattgacagc tgttgaaggt ggcgtagaca tcaaagaatt aaatgttggg
    55921 tcaatggcgc attcggtcgg taaagtcgtt gtaagtaaag tattgtctat gggacaagaa
    55981 gacgttgaag cgtttgagaa gcttgaacaa aaaggtgtga aattcgatgt acgtaaagta
    56041 cctaatgatt cacgcgataa catggatgat attttgaaaa aagcaaaagc tgaattagca
    56101 aaagcgtaat aaataatatt aaaatttata ggaggcttat catgtctatt atttcaataa
    56161 ttttagtcgt attagttgcc tttctagctg gtatggaagg tattcttgat gaatttcaat
    56221 ttcatcaacc actagtggca tgtacattga tcggtttagt taccggtaac ctcgaagctg
    56281 gtatcgtact tggcggtacg cttcagatga tcgctttagg ttgggcaaat atcggtgccg
    56341 ctgtagcacc agatgctgca ttagcatcag tagcatcagc aattatttta gttttaggtg
    56401 gacaaggtgt taaaggtgtt ccttctgcga tcgcgattgc cgttcctttg gctgttgcag
    56461 ggttgttctt aacaatgatc gtacgtacaa tcgctgttcc aatcgttcat ttgatggatg
    56521 cggctgctga agaaggaaac atccgtaagg ttgaaatgtg gcacatcatt gctgtatgtt
    56581 tacaaggggt tcgtatcgcg gtcccagcag cagcgttgct atttatccca gcacaaactg
    56641 tgcaatcatt ccttgaatca atgccagcat ggttaacaga tggtatggct atcggtgggg
    56701 gaatggtcgt agctgtaggt tacgctttgg taatcaacat gatggctaca aaagaagtat
    56761 ggccattctt cgttattggt ttcgttgtag ctgcaatttc tcaattaaca ttgattgctc
    56821 taggtgcttt aggtgttgca ttagctctta tttacttgaa cctttctaaa atgggtggtt
    56881 catcaaataa tggaggcgga agcaactctg gtgacccttt aggcgacatt ttgaatgact
    56941 attaatcttg aaggaggaaa ataaaatggc agaagaaaaa atcaaattat caaaaaaaga
    57001 tcgtttagct gttgcgtggc gctctacctt cattcaaggt tcatggaact acgaacgtat
    57061 gcaaaacggt ggctgggcat tttctatgat tccagcaatc aaaaaattat acaaaacaaa
    57121 agaagaacgt tctgcagcat taaaacgtca cttggaattc tttaatacac acccatacat
    57181 tgcttctcca attttagggg ttacattggc tctggaagaa gaacgtgcga atggcgcgcc
    57241 tgttgatgat gtagcgattc aaggggttaa agtaggtatg atgggtcctt tagctggtgt
    57301 tggtgaccca gtgttctggt ttacagtacg tccaatgcta ggtgcactag gtgcttcatt
    57361 agctatggga ggaaatatcc ttggaccaat catcttcttc ttaggttgga acattattcg
    57421 ttgggcattc atgtggtata cacaagaatt tggctacaaa gctggttcta aaattactga
    57481 tgacctttct ggcggattgt tacaagacgt aacaaaaggt gcttcaattt tagggatgtt
    57541 cgtattagct gcccttgtac aaagatgggt atcaatcaaa ttcttaccag ttgtatcaac
    57601 tgtaaaatta gataaaggtg cttacatcga atgggataaa ttgccttcag gtggtgaagg
    57661 aatcaagagt gcatttgaac aagttaataa tggcttagcc ctttctcctg aaaaggtaac
    57721 aacattacaa gacaacttag accaattgat tcctggttta gctgcattag gattaacatt
    57781 gttatgtatg tggttgctta agaaaaaggt ttctccaatc gttatcatcc ttggtttatt
    57841 cgtagtcggt gttgttggac acgtcatcgg acttttataa tccataaaaa tatagctcgg
    57901 gcgttgtccc gagctttttt tatatagtat actgttcata aacaaatatc tggagggaga
    57961 aaatggttca atcaatcaat acaaaagtag acttggtaat tgatgctact tcgtttacgg
    58021 gtctgacaga ttacgggcaa atcatgatag gtgataatgg ttttgagttt taccatgcgc
    58081 gagatagacg caaatatatc caaattccat gggaagaagt ggattatgta ctagcatcag
    58141 tcatgctaaa gggaaaatgg attcctcgat atgccattca aacgaaaaaa aatggtacat
    58201 acacattttc ttccaaagaa cctaaaaaag tgcttcgtgc gatcagagaa tatgtagatg
    58261 aagatagaat ggtacaatca ttgagtttct ttgatgtagt gaaacgtggt attcgtagta
    58321 tgtttaaacg caagtaaaaa ataaagaaaa cttctaagag gaggttttct ttattttttt
    58381 actttgcaga agaaaatatg aaatagcaaa accaattatt tgtccccttt aaatcccata
    58441 tgctaaaatt gattagcaaa gaaggaggtt atattatgag ttggttatgg acattgattg
    58501 ttggagccgt gatcggagca attgcaggtg cgatcaccag taaaggaaat tcgatgggct
    58561 gtgtaacgaa tatcattgct ggactagtcg gttcaagtat tggacaagcg attttcggcg
    58621 aatggggacc aaaagctgca ggtatggcac ttattccatc tattttaggc gcagtgattt
    58681 tggtagcagt tgtctcattt ttctttggaa aacgatcata agtttttaca aaatagtaaa
    58741 ctaaaaggag ttgcagcgag actgtgactc ctttttggct ttatttattt aaaataaaaa
    58801 gaagttttcc ggaaattttt atttaactgt cgtgtacgaa gaaaagtttg ctataatgga
    58861 aaaaagcgtg caaagaaaga gggagtttca ttatgatgct acgtgtagaa aaattgcgca
    58921 aaaaaatgca agaagaaaat ttggattcat ttttggttac cagtccgtac aatcttcgtt
    58981 acttaacgaa ctttacaggt acgacaggat tagctgttat tactttggaa aaagcatttt
    59041 ttattactga ttttcgttat accgagcagg cggcagcgca ggctcaaggg tttgaaatta
    59101 ttaaaaatgt cggacctatt tttgatgagg tagcaaactt agtacgcaaa gaagaactgc
    59161 actcattagc ttttgaggaa acaacagtct catttttaga atattcggtt ttagaagaaa
    59221 tcatcgatgc ggaattagta cctgtttcac agatgatcga agaactcaga gaagtgaaag
    59281 atgaagaaga aatcgctatt atcgaaaaag catgtcatat tgcggatatg gcatatgatc
    59341 acatcctgaa aatgatccaa ccgggcatga ctgagattga agtcgcaaat caactagact
    59401 tttatatgcg ttctcttggc gcttcaggtg tttcttttga tacgatcgtt gctagcggat
    59461 tgcgttcggc tatgccgcat ggtgtagcaa gcaaaaaaat catcgaacaa ggtgacttga
    59521 tcacattgga ctttggttgc tattacgaag ggtacgtttc cgatatgaca cgtacattcg
    59581 ctattggtga tcctggggaa aaattaaaag aaatttatca aatagtctta gatgctcaat
    59641 tagcagttat tgatgctgca aaaccaggaa tgactggtgt ccaattagat gcagttgcta
    59701 gagattatat ttcaaaacat ggttacgggg aagcatttgg acattctaca ggacatggga
    59761 tcggtttaga gatccacgaa ggaccaaacg tttcttctcg tgcagaaaag caatttgtac
    59821 caggaaatgt gatcactgat gaaccaggga tctacttacc aggaatcggt ggagtacgta
    59881 ttgaggatga cttgttgatc acagaagatg gaaatcgtgt gttgactcgt tcaccaaaag
    59941 aattgatcat cttatagttt catcaataga agaagtaagc gtaaaaatca gtgttcacag
    60001 actttgttat agaaaatcac tcgatttaat gatacactat agaagaactt ttaagctgta
    60061 agagataata aagttgggtg ttttctccaa ttcatagcag tttgcagctg atcggcggca
    60121 gttttcttac cacgtgatca ctatgccgca tcatagagaa ttcggatgta tctcgtgctt
    60181 aggcaaaggg aggaataaat aaatggcaga tgaaaaaaat ttgattttaa acgcaaacca
    60241 agatttagga gagatcgtga tcgctcctga agtcatcgaa gtaattatcg ggattgcagc
    60301 ttctaaagta gaaggcgtat acggaatgcg cggaactttt gccagcaatg tcactgaatt
    60361 attaggacgt gcagctcacg gaaaaggtgt ttacttagca aacgaggaag atggcttgaa
    60421 agtagatctt tactgttatc tagaatatgg tgtttcagtt cctaaagtag caatggaaat
    60481 gcaggaacgg gtcagccagc aagtcctttt catgacggat atcgatttat ctgaagtcaa
    60541 tatccatgta gtagctgttg ttcctgaaaa attagaacaa ccaacaatcg aagaactttt
    60601 tgaagacgaa gaggaagaga atgaataaag aattatcaag acacgagatt cgggaaatgg
    60661 cattgcaggc tttatttcca ttagacttca atgcagacct aacaaaagaa gatgcgattt
    60721 tcaacgcaat cgaattagac caccgcgaca tgatcaacga agatgaatca gagtttgttc
    60781 ctgtttatct tgatacgcta gttggcggtg tctgtgcaaa aaaagatgaa ttggataaag
    60841 tgattgaaaa acatttaaaa aacaattggc acatcaatcg gatctcaaaa atggatttgg
    60901 tgatcttgcg tatcgcgatt tttgaaatga cttatgtgac agatgtacca gcggctgttg
    60961 ctttaaatga agcaatcgag ttggcaaaaa catttagtga tgaccgttca cgtaaattcg
    61021 tcaacggtgt tttatccaat gtattgaaag aattagaagc tggggcgtaa gtcctagctt
    61081 ttttctatga atcatcataa acctgttata aatcgggaaa attctatggt agaatgaaaa
    61141 cacaaaaaat atctgggagt ggttagatgg cagaattgat taacggaaaa gaattagctg
    61201 aaaaaatgca ggcagagatt gctgaaaaaa tccaaaaatt gaaagataat ggtatccatc
    61261 ccggattagt cgttttattg gtaggggaaa atccagccag ccaaatctat gtaagaaata
    61321 aagaacgtgt agcaaaagaa atcggaatcc attcattggt agagcgttat cctgagacga
    61381 tttctgaaga cgaactactt gcagaaattg aaaaatacaa tcaagatcct agttttcatg
    61441 gtatattggt ccaattacca ttgccaaagc atatagatga agaaaagatc ctgttagcta
    61501 tccgccctga aaaagacgta gacggttttc atccgatgaa tttaggcaga ctccttgcgg
    61561 gtaaaccgga caagattcct tgtactccat acggcatcat gaaaatgttt gaagcttatt
    61621 ctatcgatcc ggcgggtaaa cgagcagtag ttatcggacg aagcaatatc gtcggtaaac
    61681 cgatggctca gctactcttg atggcagatg caactgttac tgttgctcat tctaaaaccg
    61741 aaaatctggc tgaactggca caagaagcag atattttagt cgtagcaata ggacgtggac
    61801 attttgtcac aaaagaattc gtaaaaccag gtgctgtagt gatagatgta gggatgaatc
    61861 gtgatgctaa cggtaagctg atcggtgatg tgaaatttga tgaagtggag cctattgcag
    61921 gctggatcac tcctgtgcca aagggtgtag ggccaatgac tatcacgatg ctgatgtatc
    61981 aaaccgtaaa atctgccgaa gcttttggag caggtgaata gatggcacaa gaatatttaa
    62041 ctgtcactac attaacgaag tatttgaaaa gaaaatttga tgccgatcct tatttagaac
    62101 gcgtatattt aacaggcgaa atatcgaatt tccgtttacg tgccaatgcc catcaatatt
    62161 tcagcttaaa agatgaccat gcaaaaatat ctgctattat gttcaaagga gcattccaaa
    62221 aattacgatt ccagccaaaa gaaggaatga aagtccttgt cgttgggcgt atttcattat
    62281 atgaagcggg aggttcttat caaatctatg tagagcacat ggagcctgat ggaataggtg
    62341 ctttatatca agaattggaa gaacgaaaag aaaagctgat gaaggaaggc ctttttcaag
    62401 ggcctaaaaa aatgctgcca cgtttcccaa agcgaatcgc ggtccttacc agtccaagcg
    62461 gagcggttat ccgcgatatc atcacgacgg tgaaaagaag atatccaatt gtccaattgg
    62521 ttttgttccc aacagttgta caaggaaacc aagcagcgga caatgttgtc aggaatatcc
    62581 agagagtaga agagatgggc aatttcgata caatgatcat cggtcgtggg ggtggttcga
    62641 ttgaggattt atggccgttt aatgaagagc gggttgctcg ggcgatttat caagctaata
    62701 cgccggtgat ttcatctgta ggacatgaaa cagataccac gatcgctgat ttagtagcag
    62761 atgttcgggc tgccactcca acagcagctg cagaattagc tgttcctgtt ttgtcagaag
    62821 agttgatgcg cattcaagaa cggcaagcta gattgcagca ggcgtttgta cgccagatcc
    62881 agcgcaaaca agaacggttc gagcgtgctc gacgttccta tgtatttcgg cagcctgaaa
    62941 gattgtatga aggtcagacc atcaaattag accagttgaa acagcgatta tatcaagcta
    63001 cacagcaaat ttatcatgcg aaagaaaaac aaacgatcgc attaagccac caattggaac
    63061 aagtggctcc gctttatcga gttaaagcag ctcgccaaga aactgcttat ctagaaaaac
    63121 gcttagaaga gaaaatccaa caatatatga aacaacagca gcagcgtttt gctcaagctg
    63181 ttcaatctct tgatttgttg agtccattga aaatcatggg aagaggctac agctatacga
    63241 cgttaaatga tcaagtagta aaaagtgtca ccgagattca tcaaggggat ttgctgacga
    63301 tccattatca agatggaatc gttaaaggac aagtacaaga gatagacaaa ggagaataaa
    63361 aatatggaaa atccaacttt tgaagagtca ctgcaagaat tagaaaaaat tgtcatgcag
    63421 ttagaacaag gagacgttcc attagaatca gctctagatt cgtttaaacg aggaatggaa
    63481 ttaagcaaac attgccaaga tacattagct aaagcagaag agaccttgac gaaaatgatg
    63541 acagaatcaa atgaagaagt tgattttgac ggaaacgagg aaagctgaga tgagattaac
    63601 tgacttttca gctattcatc tacctgctgt agaaaaagaa atcttgtctt ttttagatga
    63661 gcatacaacg gatagaggac tctatgatgc gatgagttat tcagtgaaag caggcgggaa
    63721 aagaatacgt cccttattac ttttaacggc cgttgcttcc tttgatgagc ctattgatgt
    63781 tcccgtctat caagtagcag ctgctttaga aatggtgcat acgtattcct tgatccacga
    63841 tgatttgcct gcgatggaca atgatgatct tcgtagagga aaacctacca atcataaagt
    63901 attcggcgaa gcattagcta tattggccgg agatggtttg cttacaggag cattccagct
    63961 aatcagtatg gcacacttag gtaattctcc taagcttctg ctgcttcagc aattagctgt
    64021 ttgtgcggga tcacaaggta tggttgctgg gcaagctgca gatatcgagg gcgaatcgaa
    64081 aaagctttct ttagaagaat tagcatttat tcatgaaaga aagacagggc atttgattcg
    64141 ttatgccttg ctggctggtg gcatattagc gaagcaacct gaagaaatct tattgctact
    64201 tcaacgatta gccgaacatt taggtttagc ttttcagata agagatgatc tactcgatgt
    64261 gattggtaca acgaaaacat tagggaaaac agctggtaaa gatgaacgta tggaaaaaaa
    64321 tacttacccg cgtttattag gcctagaaaa gacacgtgag gcgttggaga ttgagctgtt
    64381 atccgccaac aagatcatag acaaattaga agaggacgtt ttttcttttg atggcgggct
    64441 gatgcgccag atgatcaagc aatttgatgt ggagaaatga gggaaagaat ggaaaaagaa
    64501 cgcgtagatg tattagcggt caaacaagga ttatttgaaa caagagaaca agccaaacgt
    64561 tcagtgatgg ctggtttgat ttacaacgaa aaaaacgagc ggtttgataa accgggagaa
    64621 aaaattccag tgagtagtga attaagagtc aaaggtaaaa aacttcctta tgtttctcga
    64681 gggggattaa aattagaaaa agcactcaaa gcatttgatc tatctgttga aggaaaaaca
    64741 ttgttagata ttggtgcatc cactggcgga tttactgatg cagctttgca aaatggcgca
    64801 aaaatgagtt atgctttaga cgttggctac aatcagctag cttggaaaat aagacaagat
    64861 cctagagtcg tcgtaatgga aagagtgaat tttcgttatg caaaacccga agactttacc
    64921 ttaggaattc cagaagttgc tgttatcgat gtttctttta tttcactaaa actgatgctg
    64981 cctccattac atgctatttt aaaagaagag ggagaagtga ttgctttaat caaaccccaa
    65041 ttcgaagcag gcagagaagc tgtagggaaa aacggaattg ttcgtgaccc tcagacccat
    65101 caaaaagttt tagaagacat tttgtcattt gcggcacaag gtggatatga tgtgctggaa
    65161 ttaagttatt ctccgatcac ggggggagaa ggaaatatcg agttcttggc tcacttgagg
    65221 aaagtacccg aaagcggaac aatcaattca gctattaata tggcagaagt ggtttcaaat
    65281 gcacacgaac agtttgacca taaataaatt gtaactgtga aaatcaagtg agaagtatgc
    65341 ggattcttct ttattatacc aaggaaataa gctatcattg attaagaaga atatgctaag
    65401 gagcgaagac gtaatgagaa aaaaagatcg gcatcgtttg attactcgat tgttgagtga
    65461 gcaagatatc cgaaagcagg aagaatttgt tgaaatcctg aaaagcaaag gaatatctgt
    65521 tactcaagct acgatttcca gagatataaa ggaactgaaa ctgatcaagg taccggcttc
    65581 agatggcggc taccgttata gcataccagc tgaaacaaac gaagatgtaa atactaaatt
    65641 ggaaaaacta ttaaaagacg ctttcgtggc agttgaccaa atggaaaaat ttgttatttt
    65701 gaaaacattg ccgggaaatg cttcggcagc agcaaatttg attgataaac gttacaagaa
    65761 agaattattt tcgattatca atgatgacga taatgttttg atgattacac gaactgaaga
    65821 ccaggcagtt ttattgaaga aagacttcct gcattacctt taagttgagg agcgaatgtg
    65881 atgctacaag aaatcagtat tactaacttt gcgattattc cggaattgcg gctttctttt
    65941 cacgaaggaa tgaccgcgct gaccggagag actggtgcgg gtaaatccat tattatcgat
    66001 gctttaggtc tgttagcagg aggaagaggt tcaagcgatt atatacgtca aggagcagaa
    66061 aaatgtgtgc ttgaagggtt gtttgaacta ccaaaacaag aaggattctc tgaactgatg
    66121 gttgaattag ggatcgaaac agatgaggac aatctgattg ttcgacggga tatgtcctta
    66181 actggaaaaa atgtttgtcg agtgaatggt catattatca cattagctaa tctgagaaga
    66241 atcggcagtt atttagtaga tatccaagga caaaacgaac atcaggaact gctccagcct
    66301 gaatcacatc ttgctttatt ggatcgtttt ggagatgtag ctttccaaca gaagaaaaag
    66361 agttatcagc aagaatatat ctcttatcgt gagctggaaa aaagggtacg aaaagtgcag
    66421 caaaatgaaa aatcctatgt tcagagaata gatatgctcc atttccagca agaagaaata
    66481 gctgctgctg atttgcaagt cggagaagaa gagaaattaa aagaagaaag agaaaaactt
    66541 agcaattatc aaaaaattgc tgatggatta gctgcaggtt acggagcact gacgaataat
    66601 gaacaaaaca gtgtagatgg tgttggcttg gccgtttcag aactgcaagg gatcgcccat
    66661 ctagatgtgg agtatgaagc aatttatgaa aatatccaaa gtgcttacta tttgctgcaa
    66721 gatgcaatcg gcgatatgag tcgtcagatt gatttattgg agttagatga gagtcgttta
    66781 gaagaagtta cgcaacgttt agaattaatc cgacagttga aacgtaaata cggcgaatcc
    66841 attgagtcaa tattggctta ctatgacgaa atcaccgaag agctagcttc ttctgatttt
    66901 tcagaaggtc aattggacaa aatgaaagag gaactagagc aaaaagagct tctacttcag
    66961 caacaagccg cagatcttca tgaagcgcgt aaaaaaatcg caaaagaatt ggaaaaatcc
    67021 attctgcatg aattgaaaag tctgtatatg gaaaacactg agtttgaggt acgtttctta
    67081 aaagaagaaa atagacaatt gaatcatgat ggatttgacc aaatcgaatt ttacattacg
    67141 accaatccag gagagccact gaagcctttg gtgaaagtcg cttctggtgg cgaactgtct
    67201 cggatgttgc ttgctttgaa aactattttt tcttctgagc aaggtgtgac aagtattatt
    67261 tttgatgaag tggatacagg tgtgagcgga agagttgcac aggccatcgc agataaaatt
    67321 tctcaaatat cgaaatactc tcaagttttg tgcatcactc atctgcctca agttgctgca
    67381 gtcgccgact atcaatatta tattgtgaaa gcagtgattg atggacgtac gcaaacttct
    67441 gtttcagagt tgaaaacaaa agaaagagaa gaagaaattg cacgtatgct ggctggaagt
    67501 gagattacaa aactcacact cgaacatgca aaagaactat tgaaactcgc aaaaaggacc
    67561 tgagccacct cgcagtggtt caggtccttt tcagcagctg atagctatat tttattttta
    67621 catgacactc attacagcag tcaatacaac agatccaatc gttaaaatca gcattgtcca
    67681 aatgacaaat tttgttactt tagcaaaagt acttgtttgt tttttttcac gcattgtttt
    67741 caacctcgtc attaattagt tgattcaagt gtacaatctt ttttccattt ccacaagagg
    67801 gcttttttat aaaaacttcc ctttttttaa ataataggta gttacgatac aaataaataa
    67861 tgttaataga actagccata taaaggcatc atccctttca gcaataggta acttaatgtt
    67921 cattccatag atactgccga cgattgtcgg tatagttaaa ataatcgtta aagaagttaa
    67981 aattttcata atattattca gattattcga tacgattgct gaaaaagtct gattcatttg
    68041 ttctactagt tttaattgaa tgcgagaagt agtcattgcc tgctctgttt ccaccaatac
    68101 atcacgtaaa tgatttttca aagcatgatt ttcttggaaa agtttatttt gcgctagttc
    68161 tttcaatgca tctaaattag cagaagtcgc ttctctgaaa taaacgaggc ttttttggat
    68221 atccatgatt tgataaagtt gtttgttttc agtggatact tgaagtttgg cttccaaagt
    68281 atcgcattga gattgaagcg tagctaaata actgttgtaa gagagtgtga cctgccaaat
    68341 gaattgaaag aagatattga gattaggcga aatatcactt tttggaaagg tctggctaaa
    68401 gactttcttt aaaaacagtg gttcatgatt cgtaattgtg ataagcttct ttttgggcgt
    68461 caaaataata gagagcgggt atgtatccac ttgaaaataa ccgctaggac ttgttttagc
    68521 aaaaggatat tgtaacaata ataacgctgg ctttgtcaac tttgattgat tgaattcttc
    68581 tgtacgggaa ttttctgcat cgtccaatat ggaagtgatg taatctgacg gaatttcaaa
    68641 ttgatgtgtc agctgatgaa tctcttcttg attaggacgt tctacacaat accagatcgt
    68701 ttggtcagtt tcctttccgg tattacgcag tttctcatta tctaatgtaa aatatttgat
    68761 catgattttc ctcctgttgc ctgtttcttt tattataacg gatagggaat tggaatacat
    68821 ggaatgtgtt tgtaagatat agaatataac tgcataaatt taaataaaat gaaacagttg
    68881 tgtaaagtat atacagtata cttgaagcaa cgataaaaaa ggagtttacg ttcatgggca
    68941 cattcacacc acttatcttg ttttggtata tttacccggt aatcgttttg tttggttgcc
    69001 aatttcttgt aaaattattt tcgttaaatc gtcgttttaa aataaaagcc cctgatttgg
    69061 cgattccttt tttgtggggc ggtatgcatg cattgtctag aaatacaggg acaatttcta
    69121 tcttaccgtt tttctttatt tctgttttgc taatgggaat ttgtattgct gttttccaag
    69181 catattatta tgaagaaatt atttatccgc gttatttcaa gatgacttgg cgtttggttt
    69241 ttctgttgac aatcgtcttg tacgctgtct tgatcattgt gaacatactt tcttacctat
    69301 gaaaaaaata gaaaagctgt aaaaagtctt ttaaaaggct ttttgcagct tgatttttaa
    69361 aaaaatgttt atgaataaaa aaagttgttc ccggatttcc cagaaaaggt ggtagaaagt
    69421 gggggattgt ggtagactaa ttttagttag tggaggaatg gggggctttc aatgttgatg
    69481 ggtgaattcc aacataatat tgatgcaaaa ggacgtttaa tcgtgccctc aaaacttcgt
    69541 gaagaacttg gcgaaaagtt tgttctgaca cgcggattgg atggctgtct tttcggctat
    69601 ccgatgagcg aatgggaaaa tctggaagct aaactgaacg aaatgccgtt ggctaaaaaa
    69661 gacgcacgta cttttgtgcg attcttctat tcggctgcca ctgaatgcga acttgataaa
    69721 caaggacgaa tcaatatacc cagcacgtta agaaattatg cagctttgac aaaaggttgc
    69781 gtagtcatcg gtgtatctaa ccgaattgaa atttgggatg agacacgatg gcaagatttt
    69841 tctgcagcag cagaagagaa ctttgacgaa attgctgaaa gtatgattga tttcggacta
    69901 taagaatagg aagaggctga ctaatgactg aaacatttca acactataca gtcatgttga
    69961 aagaaacggt agatggattg aacatcaaac cggatggcgt ttatgtagac tgtacactcg
    70021 gcggtgccgg acatagtgaa tatttattaa gccaactaag ttctaaaggg catctgtatg
    70081 cgtttgacca agatcaaaaa gcaatcgatc acgccaagct gcgtttagct tcttatgtcg
    70141 aacaaggtat ggtcacattt atcaaagcga acttcagaga attagaagaa cgcttgagtg
    70201 agcatgtaga taaggtagac ggcattttgt atgatctagg ggtttcctct cctcagctag
    70261 atgaagcaga acgtggattt agttatcatc aagatgcacc gttggatatg cgcatggatc
    70321 agtcagctcc tttatccgct tatcacgtaa ttaatgaata ttcctaccat gaattagtaa
    70381 agattttctt ccgctatggc gaggagaaat tttccaaaca aattgctcgt gaaatcgaac
    70441 gagtacgaaa aacaaaacca atccaaacaa ctggtgagct tgtcgaaatc attaaacaag
    70501 tcatcccagc tcccgcaaga agaaaaggcg ggcatcctgc aaaaagaatt tttcaagctg
    70561 tgcggattgc agtaaatgac gaactcggtg ccgaagaggc ctctttagaa caggcaatcc
    70621 ggttattgaa agtcaatgga agaatcagcg taatcacatt ccattcattg gaagacagaa
    70681 ttgtcaaaag tatgtttaaa gaatatagta cggtacaaga tctaccaccg ggacttcctg
    70741 tcatacctga cgaattccaa ccagaactga aagtcattac tcgtaaacca atccttcctt
    70801 cagaggaaga gctaacagaa aacaatcgat cacgcagtgc aaagttacgt attgcagaaa
    70861 aaataagaat gaaagaggag tagaatgatg gcagaattaa agaaaatgca agattacgaa
    70921 tatgatttac ctgaattggt ggatcagccg gaacaacaaa tgcctgattc ttcttcgaat
    70981 caagaaatca ttcttccaca atcaccggca aaacgattaa agcagatttc tgctttggaa
    71041 aaaattgctg tggcaagcgt ggcgattgct attgttgctt tatgtgtttt aacagttatg
    71101 ctgcgcacaa atatcagcgg agctgaaaaa gaaatcacgt caatacaaac agaaatcaat
    71161 gataaaaagc aagaaaaaac gagtttagaa caagaaaaaa acgaactttc tcgtactgaa
    71221 cggattaaac aaatcgcaga agaaaaagga ttgtctatta atgacgacaa tttaaggaaa
    71281 gtgaagtaaa tgagtcttaa aaataaaatt cggcggttta tggaaaagaa aaatctaaat
    71341 ccgatgaaca atcgcaaaaa agtaggtatc attttatttg ctacgagtat tggattgttc
    71401 tttttatttg ccgtccgttt ctcgtatatc gttattggag gtcatgttgc tggtacctca
    71461 ttagctgaaa aaacaaaaca gttgtaccaa ggaagtgaag tagtaaaagc aaaaagagga
    71521 acgatttatg atcgtaacgg tgtggcgctc gcagaagatg cctcctctta ttcaattaaa
    71581 gcgatactct caaaaacata tacctctggg gataaaaaac tgtatgttga agaaaagaat
    71641 tttgataaaa tagcagaaat cctgcataag aatctttcta tagacaaaaa agatgcactg
    71701 aatattttag aggacggcgc aaaaaaagag ctgtatcaag ttgaatttgg tagctatggg
    71761 aaaaatatct cccaagaaac caagcaaaac atcgaagcgg acatgaaaaa agaaggtgtc
    71821 gctggtcttt attttgtcga ccatcaggct cggatgtatc caaatggcgt cttttcttct
    71881 cattttattg gatacgcagt tccagacaaa gatgagaatg gcttagttgg taaactaggt
    71941 ttggagtctg cttacaatga tatcttgagc ggtaaagatg gtaagattat ttatcaaaaa
    72001 gataattttc aaaatcctct gccaggtaca gtggcagaag aagaaaaagc tgtcgacgga
    72061 caagatatct acacgacttt agacagcaga ctacaaagtt atttagaaac actgatggat
    72121 caagtaaatg aagaatatca gccagaagaa ttgacagcag tcctgatgaa agcaaaaacc
    72181 ggagaaatct tggcaatggg acaacgacct actttcaatc ctgaaacaat ggaaggattg
    72241 actggaaaag atgctatctg gcgtaatttc ttggtacaag atagttatga accaggatca
    72301 accatgaaag ttttcactac tgcagctgct atcgaagaag gagaattcaa cgagaatgaa
    72361 accttccaat ctggaaaaat ccaagtagaa gatgctacga tcaatgacca tgactttggt
    72421 gaaaaaggtg tgctgaccat gcgtcaagct ctttcatggt caagtaacgt cgggatggtc
    72481 atactggaac agcgattagg cggacgatgg tataactatt tacaaaaact gggatttgga
    72541 caaagtactc attctggatt agatgatgaa gtaaacggtg ctttacctac attaaatatt
    72601 gtcgaccgag caatgagtgc ttatggacaa gcggtaggtg tgacgaattt ccaaatgatg
    72661 aaagctttca cttctattgc gaataacggt acaatgattc aacctcgata tatcagcaaa
    72721 gtggtggatc cccaaactgg agaagagcga acaactcaaa cagaagttct agggcaacct
    72781 ttttcaaaag aaacaacaga aaaagtccgt gaatatatga gagatgttgt cgaaagcgaa
    72841 aactacggta gtgcttatgg tgtatatagt gtacctggat acaatgtatc ggcaaaaaac
    72901 ggaacagccc aaatcgcttc tgatacaggg ggatatcaaa caggagatac agcctacctg
    72961 tattcgattg tggagatggt tccttctgaa gatccagact atgtcttgta tctaacaatg
    73021 aaacacccta agacatatga tcgaatggca ttggctaaaa tcgcaaatcc attgatgaaa
    73081 cgggcaatgg attttaaaga aactgaagaa gattcagata cagaaacaaa aacagaaaaa
    73141 gtatccgtag ccgattatcg taatcttgaa gcggacgttg cagcagctga tgctcaaaaa
    73201 agtggactgc aacctgtagt gatcggtaat ggtaaaaaag tacaaaaaca atctactgcc
    73261 aacggtgatc agttgatctc tggagaaaaa ctgatcctat atactggtgg tgataagctg
    73321 atgcctgacg taacaggatg gtcaaaagca gatatcatga agctggggaa aatcctcggt
    73381 attgaagtta gttttgatgg agatggttat tgtgtcaaac aagaacttgc tccatatgaa
    73441 aaaatcactg aagacaaact tagttttact ttagaagaat aagaagagaa ataggagaga
    73501 aaagagcatg gaatggacac aagcattgat tcccattgtc agcagttgtg caatgacaat
    73561 tgccgcaatg ccgctattta ttggttattt tcaaatgaaa aagcaaggtc aggcgattcg
    73621 tgaggaggga ccaaagtggc acaatagcaa agccggaact cctacaatgg gcggcttagt
    73681 gtttctgatc ggctctattt tgacaggaat ttgggttgga gcttggcaaa aacagctaac
    73741 tcctactctt ttcatcttgc tttttgtatt agcgttatac ggagtaatcg gctttcttga
    73801 tgattttata aaaatattca agaaaagaaa catgggactg aactcgaagc agaaattgat
    73861 tggacagatt ctagggggaa tcatctttta tcttgtttac cgttctgaag gttatccagg
    73921 tgtattgaat ttcttcggac tagatctacc tttaggaatc atttatggta ttttcgctct
    73981 cttctggctt gttggatttt caaatgcagt aaatctgaca gatggcatcg atggattagt
    74041 tgctggctta ggaagtatct cttttgcagc ttacggtcta attgcttggc atcagcatca
    74101 gtatgatgta ttaatcattt gcttaagtgt tttaggaggt ttactgggtt tcttcgtcta
    74161 caacagaaaa cctgccaaga tttttatggg agatgtcggt tcattagcgt tgggcggact
    74221 tttagcggca atttctataa tgctaaacca agaatggaca ttgcttttga ttggtttgat
    74281 ctatgttatg gaaacagcaa gtgtgatgct tcaagttact tcctttaagc ttacaggaaa
    74341 acgaattttt aaaatgtctc caattcatca tcactttgaa atgtgcggat ggtcggagtg
    74401 gaagattgat acagtttttt ggttgatcag cattgtgact tcgttgatca cattatggat
    74461 cgtttggtag gagatgacgg gaaatgaaaa aaatatcaac atatgaaaat aagaaagtgc
    74521 ttgtcttagg actcgcaaaa agcggagtaa gtgcggccaa attattacat gaattgggcg
    74581 cacttgtcac cgttaatgat ggaaaacctt ttgacgaaaa tccggaagca caagagctgc
    74641 tttctttagg gataaaagta ataacaggta gtcatccaat cgaattgttg gatgaagaat
    74701 tttcattgat ggtcaaaaat ccagggattc cctacagtca tcctttagta gcaaaagccc
    74761 aagaaatggg tattccggta attacggaag ttgagctagc ttacgaagta gcggaatgcc
    74821 caattattgg gattacagga acgaacggaa aaacaacgac aacaacgatg acaggactac
    74881 tactgaatgc aggagcagat caaggaattg cacgtttagc gggtaatatt gggtatccag
    74941 caagtggtgt agcgcaagaa gcaaaatcag aagataaaat tgtgatggaa ttgtctagct
    75001 tccaactcat gggaatcaca gatttccgcc ctcatatagc ggtgattacc aatatctatg
    75061 aagcacatat tgattatcat ggtacaagaa aagaatatgt caaagctaaa tggaaccttc
    75121 aaaaaaacat gacagaaaaa gattatctga tccttaactg gaatcagtca gaactacaag
    75181 aattagcgca aagaacaaaa gcacgcgtac tgcctttctc gacaaaagaa gtgttggaag
    75241 acggtgtgta tgctgacgat tactcaatat actataaaaa agaaaaaata atggaaatat
    75301 ctgaacttgg cgtaccaggt aagcataatg tagaaaatgc actagctgct atttctgtag
    75361 caaaattgta tggtatatca aatgaagcta tacgagaaac gttgcatttt ttccatggcg
    75421 taccacatcg tacgcaatat gtaggagaaa tccaaggcag aaaattttat aatgattcaa
    75481 aagcaaccaa tattttagcg actaaaatgg ctctaagtgg atttgaaacg tctaaggtcg
    75541 tgttattggc tggcggatta gacagaggaa atacatttga tgaattaatt ccttcattaa
    75601 aaggaatcaa agcaatggtc gtatttggcc aaacaaaaga aaagttgatg gatgctggaa
    75661 aaaaagcagg catcgagaca atagtaacag ccgattctgt agaacaagca gttcctttag
    75721 cattagaaaa cagtacagat ggtgatgtag ttttattatc accagcgaat gccagctggg
    75781 atcaataccc aaattttgaa actagaggaa acagattcat ggaagctgtg aaccgattaa
    75841 aatagaaaaa gtagaagcga gcgataaaaa atgaaaattt tggttacagg cggaggaaca
    75901 gggggacata tttatccagc tcttgccttt gtcaattatg taaagactca agaaccgaat
    75961 gctgaattca tgtatgtggg agcaaaacga ggtctagaaa acaaaatcgt ccctgataca
    76021 ggaatgcctt tccatacctt ggagattcaa ggatttaaac gaaaaatttc catgcataac
    76081 atcaagacgg tccgattgtt tcttaagagt atccgtcaag caaaaaaaat cctcagtgat
    76141 tttcaaccag atatcgttat cggtactgga ggatatgttt ccggagcggt agtgtacgct
    76201 gcagccaaaa tgggtattcc aacagttatc catgaacaaa acagtgtacc aggtgttacc
    76261 aataaatttt taagccgata tgtggatcgg atcgctcttt catttaaaga tgcggcttcc
    76321 ttttttcctg aaaacaaagc agttcttgta ggtaatcctc gagcacaaga agtagcagat
    76381 actaaaaaat cagatatttt gaaaacattt gatttggatc cagaaaagaa aacagtcttg
    76441 atttttggcg gtagtcaagg agcacttaaa atcaatcaag cagtatcagt ttttttaagt
    76501 gaatatattc cttcttctta ccaagttttg tatgcttcag gagatagata ttatgaagag
    76561 atcctaaata aaataaatcc tgaaatacaa aatattagtg tccagcctta tatcaaaaat
    76621 atggcagagg tcatggcttc cagtgatctg ctggtggggc gtgctggtgc aacttcgatc
    76681 gcagaattca cagctctggg gttaccggca gtgttggttc ccagcccata tgttacgaat
    76741 gatcatcaga ctaaaaatgc aatgagtctt gttcacgcag gagcagcaaa aatgattgca
    76801 gataatgaac taacaggtga atcactaagt cagacagtca acgaaatcat gggtgatgaa
    76861 gaattgcaaa agcaaatgtg ccgagcttct aaagaacaag gaatccctga tgcttcaaaa
    76921 cgtctttatg acttagtaaa acaaataatc aaaaaataaa ggcggtggtc ttagtgagta
    76981 agaaaaaaga accaaaaaaa ttgtctcatc cgtcttctga tgaaaacaat ttgactcctt
    77041 ggcaaaaggc taaccgaaaa tatctggcag aacaagagat gaaaaaccaa gagccaaaaa
    77101 aagaagacat aacattagat acaccccttt cagattctga agaagaaaaa acggttcaag
    77161 aggaacagca agaagaaagc cagtccgaga aaaaaatcga attttttgaa gagatcaagc
    77221 aaaagaaaaa cggtggacca tataacggtt cttttttgaa ccggctgccc aatttaaaaa
    77281 gtcaacggca caaagtacta tttcgtcggt tagctttgat catcactgta ttgggtatac
    77341 cgctgatttt tttgatctat tatgtttctc cttatagcaa attacaagca gttgcagtat
    77401 caggcaataa gacagtcaac agccaagaaa tcatctctga tacaaaattg tcattaggag
    77461 aaaatgtttg gggacagtat tttcatcgat ctacttatat cgatcgcctt aaaaaagcac
    77521 agccaagaat cgaaacagca aatatacatt tcaaagggat gaatgaattc gatttggatg
    77581 taacagaata taaagagatt gctttgatcg caaaaaacaa tcaatactac ccagtgattg
    77641 aaaatggaac agtattagat gaaaaagtag caaatcctac aaaaaatctc cctatcttgg
    77701 aagagttcaa agactctgca aaaatcaaag aattaacaaa gcaatacaat cagttatcat
    77761 cagaattaca aaaagcaatt tctgaaatca agtacacgcc aagagcaagc aataaaaatc
    77821 tgatccaatt gaatatgaat gacggcaatc aagttattgt aaatatcaat aatcttgcga
    77881 accaaatgaa atattattcg caagtagcca aagatatgga tgaaaaaggt gtgatcgaca
    77941 tggaagttgg tatcttctcg catccttacg gtagcactca agaaacggaa gaatcgggcc
    78001 aatcggaaga aacaactgaa agtgaagaaa atgcggatca aacaaacgaa tcaagtgaag
    78061 aggcagaaac gcaaaaagca caaacacctg gatcgtctag cacaaacgga tcatctgatt
    78121 caaatgattc aagtgaaaca agtcaaatag aagataatcc aagtacaact ccgtcaagtt
    78181 taaatgaaaa ttgataaaac aactttaaaa agtaagaatg tactttctaa aaaccagtaa
    78241 tttatggtag aattggaagc agtgaatata tctattaata agtatatttt aaaacggacg
    78301 ctagttgttc gcttgtatat ggaatagtaa gtaggaggga atcccattca tggcaaaaac
    78361 aggaatgtat gtaggccttg atattggaac gacatctgtt aaagttgtcg tggctgaata
    78421 tatcgacagc caaatgaata ttattggagt aggaaacgca aaatcagaag ggattaaccg
    78481 aggcattatc gttgatatcg ataaaacggt ccaagcgata caacgagcag tacgacaagc
    78541 agaagaaaaa gcaggtatcc agatcaaagg cgtaagtgtc ggtttgccag caaacatgct
    78601 tgaagtagaa aattgccagg gaatgattgc agttaacgga gattcaaagg aaatcaccga
    78661 tgaagatgtc agaaatgtgg cttcagctgc ccttgttcgt tcaatacctc ctgaacgtca
    78721 aatcgtgtca atattgccac aagattttac tgtcgatggg ttcgagggaa tcaaagatcc
    78781 tcgtggaatg atcggcgtaa gattagaaat gtacggtctg ttgtttacag gacctaaaac
    78841 gatcatccat aacatacgta aatgcgtgga aaatgccgga ttaatcgtca acgaaatggt
    78901 gattactcct ttagcgctga ctgaatcaat tctttctgac ggagaaaaag attttggtac
    78961 gatcgtcatt gatatgggtg gcggccaaac aacaacggct gttatgcatg ataagcaatt
    79021 gaagtttacc aatcttgacc aagaaggtgg cgaatttgtc actaaagata tctctattgt
    79081 attaaatacg tcatttaaca atgcagaagc gttgaaaata aactacgggg atgcataccc
    79141 agaacggaca tcaccagatg aagaatttcc agtagatgtg attggtcagt cagaaccagt
    79201 gaaagtcgat gaacgctact tgtcagaaat tatttcagct cgaatggaac agattttcaa
    79261 caaagcaaaa gatgcattgg accaaatcga agcattagag ttgccaggtg gcgtcgtatt
    79321 gacaggcgga gctgctagtc ttcctggtgt tgtggacttg gcacaagaaa tcttcggcgt
    79381 aaatgtaaaa ttgtatgttc caaatcacat ggggctgaga aacccggtat ttacaaatgt
    79441 aataagtatc gtggattatt cagcaaactt gagtgaagtg tatcaattgg caaaaattgc
    79501 tgttacgggt gaaacctcag cagctcgtca aatggtagtt gaacaagaaa caacaaacac
    79561 atatgagagc tatgaagcac ctgaagaaac aatctacgat gagccggaac caaaagagtc
    79621 cggagaaaat gtaaaaaata aaatcaaggg tttctttaca aatatttttg attaattcat
    79681 cctgggagga ataagatatg gaattctcaa ttgataataa tatcaatgac ggcgcagtaa
    79741 tcaaagtcat cggagtcggt ggtggcggtg gaaatgctgt aaaccgaatg attgaagaaa
    79801 acgttaaagg tgttgaattc attacagcca acacagacgt gcaagcatta aaaaattcaa
    79861 aagctgaaac agttatccaa ttaggcccca aatatactcg tggtttaggt gctggttctc
    79921 agccagaagt tggacaaaaa gcggcagaag aaagtgaaca atccttacgc gaagctttag
    79981 acggtgcaga tatgattttc atcactgcag gaatgggtgg aggaaccggt acaggtgctg
    80041 caccaatcgt tgcaggtatc gcgaaagaat tgggcgcatt gactgttggt gtcgtgacac
    80101 gtccattcac ttttgaagga ccgaaacgtg gccgttttgc tgcagaaggt atcgctcgtt
    80161 tgaaagaaaa tgtggataca ttattaatta tctcaaataa ccgtttatta gaagttgtag
    80221 ataaaaagac accgatgctt gaagctttcc gtgaagcaga taatgtttta cgacaaggtg
    80281 tacaaggtat ctctgatttg atcacagcgc caggttacgt taaccttgac tttgctgacg
    80341 tgaaaacagt gatggaaaac caaggtacag cattgatggg gatcggtgtg gctagtggag
    80401 aagatcgcgt agtagaagca acgaaaaaag caatctcctc tccattgctt gaaacatcta
    80461 ttgatggagc tgaacaagta cttcttaaca tcacaggcgg tttggatatg acattgttcg
    80521 aagcacaaga tgcttcagat atcgtagcaa atgcagcaac tggcgatgta aatatcattc
    80581 taggtacatc tatcaatgaa gaaatgggcg atgaaatccg tgtgacggtc atcgcaacag
    80641 gtatcgatga atcaaaaaag gaacgcaagt ctagccgacc agctagacaa gcacaaatgc
    80701 aatcacctgc tcaaaagact gtattagata tggatcaagc taaaccaact tcttcagaag
    80761 aagaaaacag ttttggcgat tgggatatca gacgtgaaca aaatgttcgt cctagagttg
    80821 atgattccaa ttttgacaat atcgaaaaaa aagaatttga tacattcaac cgcgaagaaa
    80881 caaaatctca tggcgatgat gaattaaata cgccaccgtt tttccgtcgt aaaaaataaa
    80941 ggagaggaag agcacatgat tgctgacaac ttgcagaaaa tcaagcaaga aattcaggat
    81001 tcgtgtgctc ttgttcatcg tgatccgaaa gaagttacat tagtagctgt aacaaaatct
    81061 gttgaatccg ctcaagctga agaattggtt agagctggcg ttatgaattg tgcagaaaac
    81121 cgagtagata aattattaga aaagaaacaa gatttatttg attttcctga aataaagtgg
    81181 catttaattg gtaatttaca gcgcagaaag gtaaaattga ttgtaaatga aattgattac
    81241 tttcatgcgt tggacagttt aagattagca gaagaaatcc agaagcggtt agagaaagaa
    81301 ttatcctgtt ttattgaagt aaatgtttct ggtgaggaat caaaacatgg aatttcacca
    81361 gatgagcttt taccgtttgt ggaatcttta gctgcctgca caaaaatcaa ggttgttggg
    81421 ttgatgacta tggcgcctaa agaagcttct gatgagcaaa tccataaagt gtttggtacg
    81481 ctgaaagaat tgcgtgatct tgttcaagca aaaggttatg cccatgcacc atgtagtgaa
    81541 ttaagtatgg ggatgagtca agactttcaa attgcgattg aagaaggtgc aacatttatc
    81601 cgtgtaggaa cagcgctatt caaagacgaa taaaggagga acaaaaatgc cgcttttaaa
    81661 cagaggagca attgctaatt ttttcggatt agctgatgag gaagagtacg aataccaaga
    81721 ttatcctgaa acaacaaaaa aggctgtaca gccaactaaa aaaaatacaa cacagaatca
    81781 atcaactcat tctgtatctg ctgcgaatac tcatgaaaaa caaaaaacgg cgacttcacg
    81841 tcctgtaacg catgaaagta ttcagccggc tgaaaagaaa aaagtaattg aactacatca
    81901 atcacctaca aaaactgtag cgaaagaaag aacagcttca agaacctcta cacaacctaa
    81961 agcaaatctt gctggaaaaa tcactgtaat ggaaccaaga gcttattctg aagctatggc
    82021 gattgctaaa cgaatcattg ctggagaagc aaccttagtg aattttcatt tagtagaaga
    82081 aaaacaagcg agaagaatcg ttgattttct aacaggaaca gtttatgcac tagatggaga
    82141 tatcaaacgt gttggagatg agatttttct ttgtacaccg actgggatgg agattgacag
    82201 ttcaaccgct caatcttttg cagatgcgaa tttgtttgat ctgtaagagg aggaactacg
    82261 atttatacta ttgcactatt acttcattta cttcaacggg cagcctattt ctatagcatt
    82321 atcttgatgg tatatgcatt gctctcttgg tttccaggcg ggtatcaatc agggctagga
    82381 cgattattag ctaagatctg cgaaccgtat atcagtttgt tcgatcgtct gcctttgagg
    82441 attggaccct tggatttcac aattttaggg gcaatcctga tcttgaactt agccgtacaa
    82501 ggtttagcac agatcgttat atggataact gtccatttag tttattgatt gaagcaacaa
    82561 aatctttatt ctcagaatag ataggagggg aaaattttgg atgcaaatgt ataccagcac
    82621 tttcgggcag atgaacgtac atttatcgat tcggtaggcg attggattga acaggttcaa
    82681 ggacaatatg caccctatct gaccgaattt cttgacccaa gacaatccta tattttagaa
    82741 acactgattc gacaaagcag tgatttgcgt ttcatgtttt atggcggata tgaacaggca
    82801 gaaagaaaaa gatgtttgat tttccccgat tattacgaac ctgtagagga agattttgaa
    82861 acagcattgt atgaaatcca ttatcctagc aaatttgcca gcctaagtca tgggaaaatc
    82921 cttggaactt tagtgggaac ggggatcaaa cgctcttact ttggcgatat catctcggac
    82981 ggtgatcgct ggcaagtatt tattgcaaat gaggtggatc attttgttgc tgcacaagta
    83041 acaaagattg gaaaggttgc tgtacgcttg gaaccaatca aatacacaga aatgattatg
    83101 ccgaaagata gctggacaca agaaagagga acagttagtt ccatgcgact agacagtgtg
    83161 atctctgaga tttataatat ttccagacag cggtccaagc agctgatcga agctggtaaa
    83221 gtaaaagtga actggacaga aaatacaaga cctgattttg tacttgaact attagatatt
    83281 atttcagtca gaggttatgg cagagtccag atccaagaac ttgaggggaa gacgaaaaaa
    83341 gaaaagtaca gggtactgtt cggtgtatta cgtaaataaa ataaaaaggt ggtttactca
    83401 tggctttaac tccattagat atccaaaata agacattccc gacaaaaatg cggggatata
    83461 atcaagacga agtagatgat ttcttagatt tagttgttcg tgactatgag gaattgactc
    83521 aaagaaatcg agaattagaa aaagcagtca aacattctga agaaaaactt gagtacttca
    83581 atgaattaaa agatgctttg aaccaatcaa tcatcgtagc tcaagatacg gctgataaag
    83641 taaaaacaag tgcaagcaag gaatccgaag tgatcgttac ttctgcacaa aacaaagcag
    83701 atgagttagt tgcgaacgct gaaaaacgtg cacatcaatt aacaactgat gcagaagaaa
    83761 aagcaagaaa aatcttgaca gatgcaacag aaaaagcaag acagcttgcg acagaaactg
    83821 aagatcttaa gaaaaaaaca cgagtattcc atcaacgtat cagcttgatg cttgagtctc
    83881 aattagaaca agtgaaaagt ccggaatggg atgaaatttt acaaccattc tccagctacg
    83941 tgactgacag ccatgaagtg atcaaagaag ttttagcaaa acagcttgac aatgaaaatg
    84001 atactgatgt aaactcagac acaggagtca tagaagcacc tatgacggca tttgatacac
    84061 aggctattga tatctcgatc gtgcctggtg aaaatgaaaa cgagtagaac agaaaagcac
    84121 tttgttattc ttaaagcgag ccgatgagag tggaagttcg gtaagacact gcatggtgaa
    84181 agatcctcta cttgagacct gcttttccga aattagagta agaagcagcg gctgacctcg
    84241 ttattggtca cagagagttt gcactagata gatgcaacta aatttgggtg gtaccacggc
    84301 atgttcgtcc cttgcggctg aacatgcttt tttgtgtccg gaaaacaaat aaaggagtgt
    84361 atggttgaat gaaaatgaaa gaaaccttgc aattaggaaa aacaaaattc cctatgcgtg
    84421 gtaatttgcc aaaccgagaa ggcgagtggc aaaaagaatg ggaagaaaat cgaatctacc
    84481 aaaaaagaca agagttgaac gaaggaaaac ctagctttgt tcttcatgat ggacctccat
    84541 atgccaatgg taatatccat ttaggacatt cattgaataa aatcagtaaa gacattatca
    84601 ttcgttcaaa atcaatgtct ggctatcgtg caccatatgt tccaggatgg gatacacatg
    84661 gattgccgat tgaacaagta ttggctaata aaggcgtgaa acgtaaagaa atgtccatgg
    84721 cagaatactt ggaaaaatgt cgtgaatatg cactttccca agtagataag caacgagcag
    84781 atttcaaacg cctaggagtt gcaggagatt gggaacatcc ttatgttaca ttagatcctt
    84841 cttatgaagc tgcacaagta cgcgtatttg gtaaaatggc tgaaaaaggc tatatttaca
    84901 aaggattgaa accaatttac tggtctccgt caagtgagtc ttctttagcg gaagctgaaa
    84961 ttgaatataa agatgtaaaa tctccatcta tttttgtagc tttcaatgtc aaagacggca
    85021 aagatattct tgatgaagat acagcttttg tcatttggac gacaacacca tggacattgc
    85081 cagcaaacca aggtattgct gtaaatccaa aatttacgta tgtattggtg gaagccgatg
    85141 gccgtaagtt tgtcgtagcg aaagatttgc tagaaacagt ccaatcagaa atcggttggg
    85201 aaaatgtaac agttctaaaa gaatttgctg gacaagaaat ggaatatatg actgcaagtc
    85261 atccattcta tgaacgtgaa tcacttgtga ttttaggcga tcatgttact ttagacgctg
    85321 gtactggatt agtccatact gctccaggac atggggaaga tgactatatc gctggtagca
    85381 aatataaact atctgtagtg tcacctgtag actcaaaagg attgtttaca gaagaagcac
    85441 ctggatttga aggtatcttt tatgacaaag caaatcctat gatcactgat ctattaaaag
    85501 aaaaaggagc tttgctgaag ctagatttct ttactcatag ttatcctcac gattggcgga
    85561 cgaaaaagcc tgtgatctat cgggcaactc cacaatggtt tgcatctatc gataaattcc
    85621 gtcagaatat tttggatgaa gtagaaaaag tagagtgggt tatcccatgg ggtaaaacac
    85681 gtttgtataa tatgatccgt gaccgcggcg attgggtaat ttcccgtcag cgtgcatggg
    85741 gagtaccact tccgattttc tatgctgaaa atggagaagc aatcatcaca ccagaaacaa
    85801 tcgaacatgt ggcaaaatta tttgctgaac atggatcaat tatctggttt gaaaaagaag
    85861 cgaaagaact tcttcctgaa ggatttaccc acccaggttc tcctaatggc gaatttacaa
    85921 aagaaaaaga tatcatggat gtctggtttg attctggttc ttctcatgaa gctgttttac
    85981 gtcagcgacc agaattatca ttcccagcag atatgtattt agaaggatca gaccaatatc
    86041 gtggctggtt caactcaagt atcacgacta gtgtggctat caatggtgta gcaccataca
    86101 aatcagttat ttctcaagga tttgctttag atggtgaagg ccgtaagatg agtaaatctt
    86161 taggaaacat cattgcacca gaaaaagtga tcaaacaatt tggtgcagat atccttcgct
    86221 tatgggtatc tagtgtagat tatgaagcag atgttcgtgt ttctatggat atcttaagtc
    86281 aagtggcaga agtatatcgt aaaatcagaa atacgatgcg tttcttatta gcgaatacag
    86341 aagatttcga tcctaaaaaa gatactgttt cgtacaatga tttacgttct gtagacaaat
    86401 atatgaccgt tcgattgaac caagtaatca aagaaatccg agaagaaggg tacgacaaat
    86461 ataacttcat gcatatttac cgtacagtta tgaacttctt gacggttgat ctatcgtcat
    86521 tctacctgga ttttgctaaa gatgtagttt atattgaagc agaagatgat tatcaacgtc
    86581 gttgcatgca aactgttttc tatcaaacag ctgtagcttt gacaaaatta ttgactccta
    86641 tcattccgca tacagcagaa gaaatctggt cattcctgaa agaagaagag gagtacgttc
    86701 agttaagcga gtttcctggt tatgaagagt ttgcaaacca agaagaattg atggatacct
    86761 ggactgcttt catggacttc cgtgataatg tcttaaaagc attggaagaa gcaagaaaca
    86821 gcaaattgat tggtaaatct ttggaagcaa aactaactgt ttatccaaaa caacaagttc
    86881 gtgaaatgtt aaaagctttg gatgcagata ttgcacaatt attgatcgtt tcaccagact
    86941 actttgaagt tatggcagca gatgaacaag tgccagataa cgctatggta tttgatgatg
    87001 ttgctattac agtggaaaaa gcagacgggg aaacttgcga tcgctgtcgc caagttcgta
    87061 aagatgtagg agtagatgaa aaacttcctc atctatgcgg tcgttgtgca aaaatcgttg
    87121 aagacttcta tcctgaagct gtagctgaag gattcgaaga aaagtaaaag ggaaaagggg
    87181 gtgtgacatg ttctttgtca cacccccttt tgttatcaat cgtttaattt tccacgttca
    87241 cgataccagt tatctggttg ccagatccat tcgaatccgt ctttttcaag tagttcgaaa
    87301 gctgctttag gtcccattga tccagctttg tagttgggaa aatccggttg tgtttggtcc
    87361 caagcttttc gaatgacatc cacgatatgc caagattgag cgacttcttc ccaatgagag
    87421 aagttcgttc catcaccatt caacgcatct aatagtaatt tttcgtaagc ttctgggcta
    87481 ttttcaacca tttctgcact atttctaaat tccaatttga ttggttccgt ttcaaatcct
    87541 tgccctactt ctttcccatt caaggataaa gaaaatcctt cagttggctg aatatagatc
    87601 gttagaacat taggtggaag tgtcgtatcg tcacatggtt catcgacaga tgttttgaat
    87661 acattaacag gaacttgttt gaatacgatg ttgatccgag tccctttttc agtcaaacgt
    87721 ttgcctgtac gtacatagaa aggaacacct gaccaacgga agttatcgat agtgaatttg
    87781 cctgcgacga atgtttcagt ctctgattga gcatccactt tgtcttcttc gcgatagccc
    87841 ttgaattgta caccatccaa ttcaccttga gcatattgcc cgcgaacgaa gttttctaac
    87901 acttcttctt cgttataaat gcggatagct ttcaatgctt tgatcttttc tgtacggatt
    87961 tctttttcgg aaaatgctac tggcggttcc attgcaagta atgctacaac ctgtaaaata
    88021 tgattttgca ccatgtcttt aagtgcgcca ctatgatcgt agtagccccc gcgatcttct
    88081 acaccgaggt tttcgccaaa agtaatttgg atattgtcga tatagcgatt gttccattga
    88141 gcctcaaaaa tattattggc aaaacggata gctgagatgt tttggatcat ttcttttcct
    88201 aaataatgat caatcctgaa aatatcttgt tctgggaata cttctctgat ttgctggttc
    88261 aattcaaaag cagactggta atcagaaccg aacggttttt ctatgatcaa acgattgaag
    88321 ccattttttg tcataatatt ttgtgatttc aaatgagaaa cgatcgttcc aaaaaattga
    88381 ggtgccatag ccaaataata gacacggtta ccgtttaatt gatagtgtcc atctaatcgg
    88441 tcagacaatt cttttaatgt ctgataatgc gttgaatcat ttacatcatg ggattgatag
    88501 taaaaatggc ttgcaaaggt agttgcttct tcatctgaag gattcaatgc acggattgtt
    88561 tctttgacaa cttcacggta gtgctcatcg ctccagggac ggcgagcggt tccgattaca
    88621 gcaaaatgct cgcctaaatt tccttttctg taaaggcgga acaaagacgg gtacaattta
    88681 cgctgggcta ggtcacctgt accgccgaat atcgtaaaca atacattttt ttcattagac
    88741 atgtggatac actcctttat attttatcga caaaaatcgt gctggccgcc ttaaagctga
    88801 cggggatttt ctttctatca ttttggatag tgatcggtcc ttcataagct gcgatgtcaa
    88861 cgatctcata ttcttcgttg atattcaggt caatagtgac taagtaatcc aacaattctt
    88921 tttcatcaag cactcgtgcg atacgcactt ttgtaccgac aggataggac tccaatgttt
    88981 gtcggcgttc ttcatggaca acttcctctt ttttaggaat cgatcctcca tgaggacaat
    89041 gttcaggatg attcaaatag ttttctaaat gatctgcaag atgttccgaa gtcacatgtt
    89101 ccaatacttc agcatcatca tggacttcat tccagctata gtctaaatgt tcaactaaaa
    89161 aaacttccca taagcgatgt ttgcgaatca gactgctagc tttttgcagt ccgcttgcag
    89221 tcaattgtac accttgataa ggggaatgtt ctacaagctc ttctttcaca agtttagaaa
    89281 tcatttcact gacagatgca gctgagacat ctagactaga tacgatttgt ttattgttga
    89341 ctttcgttgt atctccacct agttctaaaa tgattttcaa ataatcttct cgatttggtg
    89401 tcatattcga tcatcctttc atggtaaaaa accatcatta gtaagtgtaa caaaaaaaac
    89461 gttaattgac tatttttcta aataaaaaac aaaggatttt tagacgtttt tacaacaaaa
    89521 aacgaaagat gatagagaag actcattctt tcgtttataa aggatatata tatacattgg
    89581 gagggatcta tttttcagat aacaacttat atgaagtgtc tgcagattcg tcataaagag
    89641 tttgttcggg ataagaaaag ataagataaa aaccatactg atcacgtacg actaaacgat
    89701 attcatctat ttctaccaca gatgtgccaa gagactgacc gttgatcaaa aacgtgtaat
    89761 cagatgtaat ctgtaattgt tgtttttgct ggctatcact aaagaaccat gtaccttgat
    89821 actttgagag tccttctgac ttttccgctt gttttttctt tttcaagtaa ataaaacttg
    89881 agagaacggt aatgagtgaa ccaagaaaat agatcatttt ttttatcacc aaatcactcc
    89941 tttatgagaa aataatcgca tagacatatt ctttcttctt tatttcattg taccgaataa
    90001 aagctaaaat ttctctaaga ctttcgactg gtttttttac tgtttttcta caaattatga
    90061 taggatacat agtaggaaga aaggtgacat aatttgaagt atcagcaagt ggaaatagct
    90121 ttttttattg accgcccgaa tcggtttata gcacattgta tgaaaaaaga cggagaaata
    90181 attattgcgc atgtcaaaaa tacaggtaga ggaaaagagg tcctgatacc aggcgctgaa
    90241 gtggctattg tatttgctcc tggacctaaa cggaaaaccg cctatgatct gattgctgta
    90301 aaaaagaagg gtgactggtt taatatcgat agtcaattgc ctaatcggct ggcaattgat
    90361 ggtattttag acggcacgat ccttttacca ggattaactg gtccatttac ggtatacaag
    90421 cgtgaagtaa cttatcttca ctccaaattt gatatttatt tagagacggt ggtcggacaa
    90481 aaagcatttg ttgaagtcaa aggtatgaca cttgaaaata aagcgattgg tgcttttcct
    90541 gatgcgccca cactcagagg attgaaacat ataggagaat taacgtatgc cgcacaagat
    90601 ggttatgccg cttatgtctt gtttattgct cagttcgaac atcttcatct ggcaacgatc
    90661 catgaagaaa tgcaaccagc actagctgat atggttcgac atgcgcagca aagcggagtt
    90721 caaatcctcg cctataattg tcaagtatcg gcagatgagg taactgtcaa gcaggccatt
    90781 ccatttgatg tagatatccc ttttgaagat cctatacaat agaaagaagg taaaataatg
    90841 atcaacttag gtattatcgg aacaaattgg attactcatc aatttgtcca agcagcttta
    90901 agcacaaaaa aatatgaatt gacagcggta tattctagac atttggaaaa agcacaggaa
    90961 tttggttctc attatgaagg ggatatcgaa ttcgcaacag atttgaagac ttttttcaat
    91021 attactcata tggacaccat ttatattgct tctccgaata gtcttcactt tagtcaagct
    91081 aagcaagcaa ttttggcagg aaaaaatgtg atagttgaaa agccagcttt ttcgacgcca
    91141 aaagaaatgg atgaaatcat ccatttggca aatgaacaca atgtgtattt ttttgaagca
    91201 gcaagaaata tccatgaatc aggttttaaa aagattgccg attacttacc attaaaagac
    91261 gagataatcg gtgcaaattt cagctatatg aaatattctt cgcgctatga ccaagtatta
    91321 gatggagaag aaccgaatat cttctcgact catttctcag gtggtgcatt gatggattta
    91381 ggtgtttacc ttgtatatgc agctgttgct tggtttggaa tgccaaaaga agtccattat
    91441 ttcccaagaa agatttctac tggtgtagat ggtcttggat gggggatctt acgttatgag
    91501 aattttgatg tatcgatcca gccaggtaaa atcggtgatt cttatttacc gtcagagatt
    91561 tattttgact caggaacgct agtgatgaat ggagtcaatg caattgaaca agcacaattt
    91621 catgatcgcg cgcataaaga aatcattgat ttggcggtaa cagctgcaga aaatccaatg
    91681 gcagatgaag cagaggattt tgccaatgtc atggctcatc cgaaagatcc agcttgggga
    91741 ttgttgtatg aagagtggat cgagctagct agaaatgtga accaagtgat ttatgattta
    91801 agaaaaaatg gcggcatcaa attcgatgca gataatgagg aagattttta atgaacgaat
    91861 ttgtacaagt atttcccgaa aattggcaga aaaaatatga ggataaaggg tttatctcac
    91921 cttctcttat tcaacgagca gtttttcagc cactaagtaa taaacaaagt atagtagcgg
    91981 tttctcctac aggttcaggt aaaacactgg cttatctttg gcctctacta ctaaacgtgg
    92041 aaccaggtga agcaagtgcg ttggtcattt ttgcttcttc tcaagaacta gctattcaag
    92101 tagcagatgt tgcacgtgaa tggggaaaag acaaagagtt gaaagtccaa tctttggtgg
    92161 gcggagccaa cgtcaaacgt cagattgagg gtttgaagga aaaaccagaa atcttgattg
    92221 gtacaccagg aagaatattg gaattgatga aagcaaaaaa aataaaagca catcaagtga
    92281 aaacaatggt atttgatgaa gcagaccagc tatttgacgc tgggaacagc caaatcattg
    92341 atcaaattct tcatcaagct ccaacagagt atcagctagc ttttttttca gcgacagcag
    92401 atcgttccct tgaaagcatt gaaaaaatca ctgggaaaac gtttgagaca attgacgtga
    92461 cagcagagga cgattctcga aaaggactga gacattactt tttacgtgta ccgatacgaa
    92521 agaaagatga ctacttgaga cgtctcacgc atatagaaga ttttcatggt ctgattttct
    92581 ttaatcaact gactgaatta ggcgtaatgg aagaaaaatt gctgtataga ggtgtaccgg
    92641 taggaagtct ggcctctgat cagaacaagc tactccgaaa agcagcgcta gaacaattca
    92701 aaaagaaaat ggtcagagca cttttgacca cagatgtggc ggctagaggt ttagatatta
    92761 caggtcttcc ttatgtagtg aatgcagaag ttccgttaag tgaagaagct tatttgcatc
    92821 gttcgggaag aactggtcga atgggtaatg aaggaatagt aattacactc gtacaagata
    92881 ataatctaag agatttaaaa cggcttttac gtccatcaaa tataacactt gaagagattt
    92941 ttttatacgg aggtaagctg caaactgagc agccaaccaa agaagatgca gctcctgcag
    93001 taaataaaaa gtatgcgaaa actttttctg gaaaggagca gaacccagaa tataacaaag
    93061 aaaaaataag aaaaaaagtt aaaaataaat caaaaaaaga gagaaataaa ggagcaaggc
    93121 gcaaataagc gcctttttat gctatgatta tgaaaacggg aacaaaattg gttgccagag
    93181 gaggggttat gtgaaagaac gtcaagaaga gatcttgcag ctattgacag aaaaccaaca
    93241 aggattgaca gcaagtgatg tagctgagcg cttaactatc gatcgaagca atgcaagccg
    93301 atatttaagt gaactataca aggcccatca tattgtaaaa accgctggcc gaccagtcgt
    93361 ttactctttg ccaacggaaa agtcaaaatc tgatgaagtg catgtcgact cttctacgca
    93421 agtgactttt gaaacactcg ttggagaaaa tgattcttta aaagtcagca tacagcaggc
    93481 aaaagctgcg attctttatc caccgcgggg gttgcatacg attatttttg gagaaactgg
    93541 tacagggaag tcgatgtttg ctgaatgtat gtatcatttt gcaatcgatt cagagatgct
    93601 ttctgcagat gcgccatttg tttctttcaa ttgtgcggat tatgctcaaa atccgcaact
    93661 gctctttgga cacatctttg ggatcaaaaa aggggcttac acaggagcag ctcaagatag
    93721 tcccggtcta atagccaaag cagacggcgg tatattgttt ttagatgaga tccaccgttt
    93781 accgccggaa gggcaggaaa tgctgtttac tttcattgat aaaggtattt accgtccgct
    93841 tggagaaagt gcacaagtac atgaagcatc tgttcagatc attggggcga cgactgaatc
    93901 ttcagaaagt ttcttatcta cgttcaatcg acggattcct atggcaatca ccttgccgaa
    93961 tcttgcttca cgttcactgg atgagcgtta tgaaatcatc tctcttttta tcaaacaaga
    94021 agcgaatcgt ttgaatcaac gtatcgatat cgaaaaagaa gcgattcttg ccttcatgct
    94081 ttatgatgcg gaaggaaata tcggtcaagt gaaacgagac ttgaaactag tctgtgcaaa
    94141 atctttttta cattaccgta ctcatcatga gaaaaagcta atcattcgaa aagaagaact
    94201 ttcccttcaa gtacaaaaag gattactgaa aatcaaggaa ataccggaac gtctcgaccg
    94261 attcatggac agcaaaagtc agtatttgac atttgaacca ggatttgctg atgttgtctg
    94321 gtcacaagat ccagaacgaa acatgcaggt ctataacgat attgaggaaa aagtcacttc
    94381 tctttccgaa accggtgtgg agaacattga tctagagacc ctcatttcaa aagatgtgga
    94441 tgcttatttc cagacatatg tcaaagaatt gactaaaagt tcgattccca aagatctttt
    94501 gccagatgaa atctggcggt taaccaatca attgtatgat gtggctgaaa aacggttaga
    94561 ccgtatttat aatgaaaaag ctcgttttgc ttttgctttg catctgcaaa gtacattgga
    94621 tcgtgtgaga gaaggtcata tgatcgtcca cccagatttg aataacgtac ggaaaaattt
    94681 taagagggaa ttccaggtag caatcgatct ttcaacgatc attgaagaag aagaacaagt
    94741 tgagattcct tttgatgaaa tcggctttat tagtatgttt ctttctatca atctaggaga
    94801 aagtgagcct gttaaagaaa acaaagtaga tgttctcata ttgatgcacg gcagacagac
    94861 tgcttcaagc atgttggaga tggcacaaga actattagga acaaaagtag ggaaagcttt
    94921 taatatgcca ctggttatgg aagttcaaac aatgtatgaa gaagtgttac aatatgtcaa
    94981 aaactatcaa gagcatttga cgaatggcct actcttgtta acagatatgg gatcattaaa
    95041 caccttcagt gatttgattt atgaagagac tggcattcgg acgaaagcta tcacgatgac
    95101 aagtaccatg attgtgatag aagcgatacg gatggcaagt gttggaagaa gcttagaaga
    95161 tatttatcaa aatatccagc tttcctttga atcgatcgta cgtgatcagt tcaaagcgga
    95221 aaagtctgaa actttacagc gtaaaaaagc cgtaatcgtg acatgcttta ctggtgaagg
    95281 agtagctgcc aaattatatc agcgtattat gccagtgatt aatcaaaata aggtagaact
    95341 gatccagatg cagtttttag aacgtgaatc attcaaaaaa cacatcgatg ccttattgga
    95401 agaatatgag atcaaagcaa ttgcgggaac agttgatgtt gattatcaaa atatcccatt
    95461 tttttcagcc tatgacgttt ttgatgatga aaaactaaat attttaaagc gtatagctga
    95521 tgatgagatc ccgacagcta aaattgtcga ttcacttgct ggatcattaa gcgatgtctt
    95581 ttctattgat gaactgatcc gttcattgca aaagacagtt cagcagatcc agaccgattt
    95641 gtcgattatc gtggaacctg gagtagatac tggaatcgtt atccatttag ccttcatgtt
    95701 agacggattg atcaaacaag aaagccatcg gcaatttgag aaactgtcta ttttctcaaa
    95761 aatttatcgt ttggaaatgg atatcgttcg ggcaaacttg atggcactag aacgaaaata
    95821 tcaggtaaaa attccagaag atgatatcgc atttcttaca caaatgtttc ttgaaaataa
    95881 aataaaaaaa tgaacacact aatcatactg tttacacact aaacatctgt gtgcgcacta
    95941 taaaaaagag ctaaaaagca tgaaaacgct atttcagctc ttttttttat tggcatgctt
    96001 cttgcgttat ataaaagtgt aaaaaaataa aaggagtgtt aaaaatggca aaaaaaacaa
    96061 tcatgttagt ttgttcagca gggatgagta cgagtttatt ggtcacaaaa atgcaaaaag
    96121 cagcagaaga aaaagggatg gaagccgata tttttgcggt ctctgcctca gatgcagata
    96181 ataatttaga atctaagaat gtcgatgtat tattattagg tcctcaagta cgtttcatga
    96241 aagctcaatt tgaacaaaaa cttgcgccta aaggtattcc attagatgtc atcaatatgc
    96301 aagactatgg catgatgaac ggagaaaaag ttcttgcaca agcagaaaat ctaatgaaat
    96361 aaatcaagta tgcagttggg ggtataacaa cgtggaagat caacaaaatc tagaagccat
    96421 catggggtta atcatgtttg gcggtaatgc aaagagcgat gcaatggaag cgattgcggc
    96481 tgctaaaatc ggtgattttg agttagcgga taggaaaatc gctgatgcag aagaatcact
    96541 tatacaagcg catcatgcac aaacagacat gttgacacaa gaagcacaag gaaatcatat
    96601 gcaagtgaca ttattgactg ttcacagtca agatcactta atgacatcta ttgcgtttac
    96661 tgatttagca aaagaaatca tcgaacttta tcgtcgcgta tacagcgaat aaatacaact
    96721 tcttttttga acggaagaag aaaaattttt gaacggattt tttattttgt tataaggaga
    96781 actctcttta tataaaaaac ttataggagg aagtaaaaaa tgaacggact atcaagttgg
    96841 atggagaaat acatcctgcc ggttgccgca aaaatcggag cgcaaaaaca tttagttgct
    96901 ttgcgtgatg cttttatcgg cacactgccc gcaacaatgg cgggatcagt cgctgtaatg
    96961 atcaatgcaa ttattcgtga tttaccgcaa cagtttaaca gtacctatgc tgacagttta
    97021 gcagcagaca aaggaatctt tgccgtattg aatgtcatca tcggaatcaa tggttttgtc
    97081 tggaacggta cactagcaat cgccggattg atctttgcct tttcttgggg gtataatctt
    97141 gcccgagctt atggagtaaa tgatctagct ggggggatcg ttggtttagc taccttgatt
    97201 caaggtattt cctttggtcc agcattatca gcagcattag atgtgaatgt tccttcaaat
    97261 gtcgcagatg caatcaatag ttctgagatt ggtgcaacga ttgcagatgg cacactgtca
    97321 gttggattat ggggttggct aaaactagat catttgaatg gaaacgctta ttttacagtt
    97381 atgatcatgg gagctatttc agtcattatt ttttgtaaat tgatgcttgc caacattacg
    97441 atcaaaatgc ctgattcagt accaccagca gtatctaaag catttgcagc cattttacca
    97501 gcgacgatcg ctttatacgt cattgctatt atcaattata tcgtaggtcg tgtagctgat
    97561 gggcaactga tcattgattt ggtgcaaaaa tatattgcag agccattcct aggattatct
    97621 caagggttgg gagcagtatt gatcgtcaca gtatttgttc aagtattctg gttctttggt
    97681 attcatggac caaatgtatt ggcaccagtg ctagagggaa tctggggaca agcacaacta
    97741 atcaatattg atattttcca aaaaggttat aacggaaaaa ctggtacacc agccgtgtta
    97801 gatgcaatcg aagatggaaa agcttatatg tgggtccgcg gatcgttcga tgcttttgca
    97861 tggtttggcg gatcaggcgg aacgatcgtc ttactgatag ctatcttgat tttctctaaa
    97921 cgtgcagatt atctaacagt agctaaatta tccattgggc ctggtatctt caatatcaac
    97981 gaaccagtga tgtttggtct gccaatcgta ttgaatgcaa tcatgtttat cccattcttg
    98041 attgctccag tagtagcaac agcaattgga tggacagcta cttatcttgg cctagtcgct
    98101 ccggtatcgc agcaagtcac atgggtagta ccgccgttct tattgtcatt cttagcaaca
    98161 ggagcggatt ggcgggcacc gatcgtaaca ttagtatgta tggtcgtgac tttcttgatt
    98221 tgggcaccat ttgtcatctc tgcaaacaaa atggaccctg ccgcaggcga acaacaataa
    98281 gatgaacgtg aggagcggga cgctttaagt ttcgttcctc ataatgaatt ttcgaggagg
    98341 aactgaaatg attacaattg ccagtgcaat gcaacaagat gaaatcaaag aattgttaga
    98401 aggatacaat gaagaaggaa tgacttttac atttaaagaa aaacaaggaa tcaagctgtt
    98461 ctttgaaaca agtgcagaag atccggaagt tgcggctaaa aaagcaaagg aactaatcaa
    98521 agcacagcct tggggaacag tcctttattt tcaatcaaca gctgtttaga aaatggttta
    98581 aaaagatagg atggttgaga tgataaaaaa cggtttattt ttatgtagta ttggttcttt
    98641 gatcgtgttg tatagtttga atccacattc gcaaaggtat gatttgtata gtatggtcac
    98701 aggtcttttt ctaacaggat taggaatcta tttcttcatg aaggggaaaa aaagagaaga
    98761 agcaaaggag aaaaataact aatggaaatc acgaaaaaat taaatattcc agcatcgttt
    98821 ttttacgata aagttatgga ctctgtcctt ttcgatgtac gtaaagcgac cggcaaatca
    98881 gtaactagaa aacagctgaa aaatttggag tacgtcaaac aattctcgca aaataaccgg
    98941 gctcgcataa aaatcgaaga agtcattgaa aaccagtctt ataagtttcg tacatcgaca
    99001 acgaagaatg aattcgtcgt tcattatcaa atcaaaccat tggacgattc gacctgtgag
    99061 gttcattaca cagaaaaaat ggagtcttat ggcatgcttc aaaagttgaa tgatatgctt
    99121 ttaggaacaa tcttgatgta ttttaaacgg agacgtttca aaaaaatgct agatatgatc
    99181 gaagcaacgt attaaatcaa tgaaacatgg tactatttag ttattgaaaa atgaagtaag
    99241 ctagaaggaa tttttcatag gaagatagat ctttcctgcg aatcctcact agcttttttg
    99301 taggaagggg cagcaaaatg acaaacaaag taagagagct aggtgtatct tcgattgagg
    99361 aaatgtttga tttagccact tatgcattta atggggcaga tacacctgaa aggcgagaac
    99421 gttttcgtac acttgcggag aattcttgga attacggatt ttttgatgag gaggatcggc
    99481 taacaagtca agtcatggcg accccttttc cagtgaattt ttacggacaa gagtacctaa
    99541 tggccggcat tggctatgtt gcttcttatc cagaagcaag gggacaaggc gggatcaatc
    99601 agatcatgga aaagatcctt gaagattgcc gtgatcgtaa agtaagctta tcttatctgg
    99661 cgccattctc ctatcccttc tatcggcgct atggctatga acaatcattt gataaaatca
    99721 gttatcaact aaacagtcgt gatgtgccgt ttatcaaaaa aaaatctgga aaaataaagc
    99781 gtatggattt tgaagatgca aaagctgcaa tcagaagaat ttatgaacag atgccggaaa
    99841 accaacgagc tggtctgaag cgcgaagact ggtggtatac gtataaattc aagcaaaaag
    99901 gcaattacca gtttgctttg cattttgatg ataatgagga agcaacggga tatatcgttt
    99961 atcagcttgc aacgtcttcc tttattattg cggagtgggg atttttggat catgatgctt
   100021 tttgcagcct tgttcgcttc gtaagttctc acaatggtgc ttttgaaaca tttgaatatt
   100081 catgcggcta cggcggtaat gatcaaaatt atctattaga aaatccattt gcttctgtaa
   100141 tgatcacacc ttatatgatg gcgaggatcg tagatatttc tttgtttttg gaaaattatc
   100201 catttaaaaa gagacaagct gcatttgccc tacaaataga agaagatcat tatgcgaaat
   100261 ggaatgaagg aatcgtggaa gtaacaatcg aaaatggaaa aaatcatctc tcaaaagtag
   100321 aacaaacaaa actcccaatc gttcgaggaa gtatccaaca tatcacgcag ctattattag
   100381 ggtatcgtaa agtggaagag ttggtattcc aagaaagaat acaggtgaca aaagaatatg
   100441 aagaaacgtt aagctcaatc ttgccacagc aacagccaat tcttaatgac tatttttgat
   100501 aaaaaacggg tgatgacaat atttttgtca ccaccctttt gtttttcatt tggcatttga
   100561 aggaaaatgt taaagtaaat cctctttttt cgtaaatttg aattttttgt tttcttttgt
   100621 tttagggttt tcttttttgt ggtcttggat tgcttctttc gtaatctttt cccgattttt
   100681 tttagatgaa tctaagcgtt ctgcgtatac tttttctttt ggcttatcat tcattggaaa
   100741 ggacctccct acattatttc attctacctc tagtttatca tctgttaagg actagtggaa
   100801 acgttttgcc taacttataa gctgattttt ttagcagata agcttgcaag taaaaaacaa
   100861 acaagctaca attgatttat tgaaattgtt cctgtagctc agtaggatag agcaatcgcc
   100921 tcctaagcga taggtcgctg gttcaattcc agtcaggaac gtacaaataa cccctgtatc
   100981 ctttgcggta cagggggtta ttttgtataa gtagacattg tagtttctaa actgctatgt
   101041 cctaaacgtt tcgatacgct aagtatattt actccttgat aaagtaaaat agatgcatgc
   101101 gtatgtctta gtccatcaat aaacagtcga agagaaaata gggaaaaatt gattactaaa
   101161 gaaaaactta ctaggtcgac ggtgtagttg attttcatac ttcaatagat tcgaattaaa
   101221 cgatttcgat tggcttatac cagtcctatc caacgaaaga tatatcgttc ttaactctag
   101281 cggtataact tgaagcttta cgtaaagcca atgggaatga actggagatg actttttatt
   101341 atttttagta atagctgatg ggttaatact tacaactttc cttttcaaag taccatataa
   101401 aaaaacggtg gggttgttta gcgaaataaa ggatctaagc caatagctta gatcctttat
   101461 tctatgcttt atattatttt ttgcatgtgt gcgaaagtat actcaagccc aatcgccatt
   101521 tcggaaaatc ggtactgtag agccatcttc acggattccg tcgatatcca tcttgtcaga
   101581 tcctaccatg aaatctacgt gggtttgact tctattcaat ccagcttctg ctaattcttc
   101641 atcggacatt tctgtaccgc ctttcacact gaaggcataa gcagaaccta gtgctaagtg
   101701 attcgatgcg ttttcatcaa ataatgtatt gaaaaagatg atgccagatt gagaaatagg
   101761 tgaagggtca ggtacaagtg cgacttctcc taaacgacgc gcaccttcat ctgtatcaag
   101821 cagttttgct aagacatctt ctccttgttc ggcagaaaaa tcaacaactt ttccatcttt
   101881 aaaagtaaat ttcatacccg agatgatggt gccagcataa ctcagtggct ttgtacttga
   101941 aatgtaacca tctactcgat gactgtcagg tgctgtgaac acttcttccg tcggcatatt
   102001 tgccatgaat ttttctccac gagcattata gcttccagcg ccttcccaaa gatggttttt
   102061 aggcaatccg ataatgatat ctgtaccggg agcagtatag tgaagggcag aaaattgttc
   102121 tcgattcaat tcttctgctt tttttgctaa tttttcatca tgcttcttcc atgctaagac
   102181 agggtcttct tcatagacac gagtcgtttt gaaaatctga tcccataatg cctcaacttg
   102241 tttttcttct ggcagttctg ggaagacttt tgctgcccat tgtttaccag ccgcagccac
   102301 gacagtccaa ctgactttat ttgcttgagt tgccttgcgt aaattcatta gtgctttgcc
   102361 attcgctgat tgataagacg ctactcgatg gctatctaca ccagcaaatg catcgggatc
   102421 ggcagaaaca acgctgatcc tgcttgctcc tttttcaagc cattcatccg cttgatcgat
   102481 tttagattgt ggtacattct caatacgatc cgttgcagca tgtaaaagaa attctctttg
   102541 gatctggtcg tcagtccatt gaacgataac ctcagcggct cctagtttat aagcttcttg
   102601 tgtgatcaat cgagcaaggg gtgcctgttc cacgctgatc tgtaaaacaa ctgtgtgtcc
   102661 tttttctgtg gctacgccag tttctgcaat taagcgtgca tattttttta gaagttcatt
   102721 aaaatcagat aacataataa aaattcctcc tcaccatttt ttctggtaac tattagcatg
   102781 ataccattta attatgattc aaacaatgag gctttaaata aaaaaaggag gttatttttt
   102841 taaggttaag tttcatgata tatgttacta cttgctattt cagcttgtga agaaaatcaa
   102901 aaactaattt tgcatatcaa aaaaacatgt tacaatgaaa ttaatcaatg gtggaagcgt
   102961 tataaaaaaa ttaaggagat taaaaaatat ggcaagaaaa aagacgatta ctagagaaca
   103021 gattttggca gctgcttatg aagtagctgc caccgatgga tttacacgat ttactgcgag
   103081 aaacatcgcg aacaaaatga attgttccac gcaaccgatt tatcttgagt ttaaaaatat
   103141 ggatgacttg agacaagctc tttttgagca gatctatgat catttacaat atgaagtgtt
   103201 cactgttgag catacaggga atacgattat cgatcttgct tttaattata tccattttgc
   103261 aagaagagag aagaagttgt atcgttcact gtacttagaa gaatatggtg gaggaaaaga
   103321 gatgcagaat ttctcttata cttactttgt gaatacagtg aaacgcgatc cagcttatgt
   103381 gaatctttca gaagagcaga tcgaatcttt gtataatggg acatggatca tagctactgg
   103441 gatcgcagct ttgatgtctt caagcatcat ccatccaact gatgaacaga ttgaaaagat
   103501 gatccaagac agtatagatg ctatcttgaa attcaaagaa ccaatcgaaa tcgattgaat
   103561 atactcaaaa gttccacaaa tcagtaggta acaagagatg tgtcaaaata aggaaaagtt
   103621 cgtttttctt acagcgtatg catttttaac tcccgaagaa ctcttccatg aatgcaatga
   103681 ataaaagagc catacagatt gaataaatct ggatggctct tttattgcaa gtatattaaa
   103741 tttttgcgac tagttcttta gcgaaagctt ctaaattttc gatatcttct tcttcagcat
   103801 tcagatccac tttaacattt tcggctcctt ttgatgcgcc tgtttttgca aaagcagcat
   103861 cgaaatcatc caccgatttg cagaattcat cgtaaaaagt atcaccagat ccgcagacac
   103921 cgtaaatttt tcccaataga tccaattctt gcaagtcttc ataaaagtct acgatctcat
   103981 caggcagatc gccatcatcg tatgtataag tagctactac gcaaatatct gcctcctcga
   104041 attcatctgc atctacttgg gtacattcgt tgatttcgac ttcgatatcc agattttcaa
   104101 atgcttctgc aacaatatcg gcgatttctt cagtgtttcc tgtcatgcta gcatatacga
   104161 tttttgctaa agccatgatg tgcctcctcg tttataatta tttaacaatt atgaattata
   104221 ccaaaacatc attgaaaaga aaataaaacc taaagacacc tggggaatgt catttggcta
   104281 aatgtatgcc gctatgttaa aatgagaggg atttgtaaaa aggagacgaa aatatgatta
   104341 ctttaaaatc agcacgtgaa attgaaatga tggcagaatc tggagcttta ttagcagatg
   104401 tacataaaaa tctgcgtgat tttataaaac caggaatcac aagttgggat attgaggtat
   104461 ttgtcagaaa ctatatcgag agtcatggcg gaattgctgc gcaaattggc tttgaagggt
   104521 atgaatatgc gacttgtatc agtataaatg atgaaatttg tcatggattc cctcgtaaaa
   104581 agcctttgaa aaacggtgat ttggtaaaag tcgatatgtg tatcgatttg aaaggcggca
   104641 tttctgattc ttgttgggcc tatgtagttg gagaatctac acctgagatc gaccgattga
   104701 tggaagtaac gaagaaagct ttgtacatcg gtattgagca agcacaagta gggaatcgga
   104761 ttggtgacat cggtcatgct atccaaactt atgtagaagg cgaaaatcta gcgatcgttc
   104821 gtgattttat tggtcatggc gttggtccta cgattcatga agaaccagca gttcctcatt
   104881 acggagaagc tggaaaaggg ttgcggttga aagaaggcat ggttatcacg atcgaaccta
   104941 tggttaatac cggaacttgg aaaatgaaaa tggatcctaa cggctggact gcgtatacaa
   105001 gagacggcgg actttcttgc caatatgaac atacattggc tattactaaa gatggtccaa
   105061 gaattttgac atcacaagga gaagaaggca cttattgatc tatccagtca ataggtacgg
   105121 gagaggtaca tgaaagtttg gaacaaaata aaacaaaatg aaaatctgat gcgctttatt
   105181 gagacgactc aaaagcgcat cactgattca gaaatgagta cgacctctgt cgtcgttgct
   105241 tattatttac tcttgtcttt atttccattg attattgctt ttggtaatat tttaccgttt
   105301 ctccacatcg atcaagagac tgtactgacc tatttacgag aagtgattcc tgagaccgtt
   105361 taccagttta tcggtcctgc aatcaaagac cttttgacac aaagttcagg aggcttgtta
   105421 tccgtttctg cattagcggc tttatggtct gcaagtcaaa gtatcaatgc tttgcaaatt
   105481 gcgatgaata aagcgtatgg cgtagaaaat agaaaaaatt ttttgatcgt ccgtttcttc
   105541 tcactaattg tcattgtctt gttcatgatt gctattagtg gggtaactat cgttcttggg
   105601 ttgggacagc tgattttgga tgctattcag ccaatcgtac aaatacccgt gaactttatc
   105661 gatcagttcc aaactttgaa gtggccaatc acattggtag ctttatttgt catcatgttc
   105721 ttgatttatt taattgtccc taatgctcag ttgaagttga agacgatcat tccgggcacg
   105781 atcttcgcta ctgtcggctg gatgctgtta tcacaagtgt ttagtatcta tgctaggtat
   105841 tttgcaacga ggatcagcgg ttatcagatc ataggaagtt ttattgtact catgctttgg
   105901 cttaattttg ctgctacgat catcattttg ggcggaatca tcaatgcagt tgttcaagaa
   105961 tacgtgacgg gtcatcaaat caaaaaacgt cgagatccaa gccgaaaatg gttcaataaa
   106021 ttgaaaacta aattttctca taaaaaaaag tagaaacaag agttcttatt gtaaatttat
   106081 atcagtaatt tataataatt atgactctta attaaagtag gtgcccatat gcttgtttct
   106141 tttagtatgg ttataagatt ttttctgaca attttattat cactgatatt aacacctgta
   106201 ttcaaaatca tttcagtaca aacaggtatg gtcgacaaac caaatgaaag aagaataaat
   106261 aaagttccaa tgccttcagc gggcggactg cctatcttta tttcatttgt tatatcgact
   106321 ttattcttat tccgtaaaat tatccctcaa tcgtatattg taccgatctt gattgcttct
   106381 ttagtaatca tagtcacagg agtgctcgat gataaatatg agctgacacc taaacaaaaa
   106441 agtataggga tcttgattgc ttctcttatt atttattttg tagcagatat acgtattgat
   106501 tccttcacgc ttccttttat tggatatatc caactaggct ggcttagttt tcccgtaacg
   106561 attttttgga tcttcggtat cacgaatgcg gtcaatctga ttgacggttt ggatgggctg
   106621 gcggcaggga tttcattgat cggtttaatt acgataggaa tcattggata ttttttcctc
   106681 catgcttcta cagtgtatat tccagttgtt attttctgtc ttgtagcaag tattatcggt
   106741 ttttttcctt acaattttta tccggcaaag atttatttag gcgatacagg agctctattc
   106801 ctagggttta tgatggccgt cctttcacta caagggctga agaacgtgac ttttgtttca
   106861 tcgatttctt tgctgatcgt catgggagtg ccggtaacag acacgttttt tgctatcatt
   106921 aggagaaaag caaaccgtgt ttctttttct acagcagata aaaaacatct tcatcaccga
   106981 ctaatcagtt taggctttac tcataaaggt gctgtcctga cgatttatgc gatggctttg
   107041 atgttttctt tcacagctat ggtcatgaat tatactggaa gaatcggaac gatcgtattg
   107101 attattgcca tgttgtttgc tgcaatttta ttatttgaat tgattggtct gatcaatgag
   107161 aaacatcagt ttattttaaa aacattgcga tttttaggga ataaagagta ccgtacgcaa
   107221 gtaaaacaga aatatttgaa gaaatggaac cgtcatatag atgaataaca aattaaacca
   107281 gccgatcctt attagtattg tgacggcaaa cagtgataaa atttttcgga cactagataa
   107341 ttttatttca actgtaaaag atgaaacagc agtaaaaatc cgaatttttg acaacaattc
   107401 acttcccgag tacaaaaaaa gactaacaga gtataggaaa tatccctaca tcgatatcta
   107461 ttttcatgat gaaaattccg gatttggtta tggacataat cacaatctga tagctagcga
   107521 ttctcgctat ggcattattt gtaacccaga tattttagtg gatgaagaga cagttacagc
   107581 attggtcttg ttattgaaag aacatccgga atgtgcgatg ttagcaccta agattcttaa
   107641 tgaagatgga acaactcagc acctcatccg tgagcagctg gctgtttttg attacgtttt
   107701 gcgctttgtg cctttcaaat gggtcaaaaa aaagtttgac aaacgactgg ctgtgtttga
   107761 atgccgagga ttgcctactg atgctaatag ttatgtacgg atgatctcag gtagttttat
   107821 ggtagctgat attgataaat ttaaagagat aaaggggttt gatgagcgtt tcttcatgta
   107881 ctttgaagac aacgacttgt gtttgagttt tgaaaaagca ggttacaagt tattgtacac
   107941 acccttttat gcagttatcc atttttacgg gaagggtgcg catcgaaatg caaaactgtt
   108001 tcgcgtgttt ttgacttcta tgatgaaatt tttcaataag tgggggtggc gttttttctg
   108061 atgaaaccag aaatattagt agtcgttgtg ctttatcagc aactattcag tcaatctcca
   108121 gcttatggac cattaagcaa agcacttagc gaaaagcaaa ttcagcttat tatttacgat
   108181 aatagtcctc aaaggcagca aaatgagctg tttgaaaaag aaaatatcgt ttatcatcat
   108241 gatccgcaaa atcctggttt agcggtggct tataattttg cgttgtctca agcagatgaa
   108301 ggaacacgtt gtctcgtcac tctggatcag gatacgaggc tagtcgacga ttattttgaa
   108361 ctattgcgta aagtaacctt tacagatgaa tgtgttgctg cagttccgat gattttttct
   108421 gggagccgac aagtttctcc cctatattca gatcgttata tcaatcgtac agctcaagca
   108481 gtagaagttg aaggcccaac agctgaacga atcatggcaa tcaattcagg tgttgcctgg
   108541 tctgtcaaat ttctaaaaaa gattggcggg tttaatcctg ctttttcttt agattttttg
   108601 gatcattggc tgttttggaa agtggatcag ttggaaaaaa ctgtggagat ccttcctgta
   108661 cgaatggaac atgatctttc ggttttagac tatgaaaaag tttcaagtaa tcgataccgt
   108721 tctattttac aagcagaaaa tcgattttat catgaatatg atcaaaggca tttatccagt
   108781 caccgacgac aattattgtt aagaacagct aaacagttct tgaccgtcaa aaaccggcat
   108841 atctggcgga tgactttgcg ctcatatgtt aagaattgga aggtgtgaat tcgtgcgttc
   108901 ggtttgtata gctaccttta atggaggcag atatttaaga gagcagttag atagtatttt
   108961 atctcagctt tcagcagaag atgaagtagt catttctgat gatgggtcag tagatgatac
   109021 atgggatata ttaactgaat acgcagaaaa agataagcga atccagcttt tttcggggcc
   109081 tcgtcaagga ttgatcgcta attttggtta tgcgatccag cagacgaaag gagatctaat
   109141 ctttttggca gaccaagatg atgtttggtt ggagacaaaa gtgaaaaaga tcactgacta
   109201 ttttgagcag aatccggatc agctagtagt agtttctgat ttaatcattg tggacagcga
   109261 gctgcaagag atccatgatt cgtatttttc gtttcgaaat tccaaagaag gatggcttaa
   109321 taatgtgata agaagtcact atattggtgc tggtatggct ttccggtctt ctttaaaacc
   109381 cgatattttg ccgatcccgt cagaagtgcc gatgcatgat atgtggattg gtttgctggg
   109441 aggaaagaag actgggttcc taagagaacc gttaactctg taccgaagac atgatttaaa
   109501 cgcgagtgag attgaaacac attctaaatt acggcaaaaa atttcttggc gtgttcatct
   109561 gattacggcg ttgatcaaac gaaaatggtt taaacgttga taaaagatgt ttaaatcctt
   109621 aatatttcca tgacaaatat aacaattgat gagataataa caaagtgaaa tttgaaatca
   109681 aaaaggagag cagatatcca tgaagggaat cattcttgct gggggcagcg gaacacgtct
   109741 gtacccttta acaaaggcaa catcgaaaca attgatgccg atttatgaca aaccaatgat
   109801 ttattatcca atgtctactc tgatgttggc gggaatcaaa gaaattctga ttatttcaac
   109861 gccacaagat acaccacgtt tcaaggaatt atttggaaac ggaaatgatt taggactgca
   109921 gatcgaatat gcagtacaag aaagtccaga tggattggca caagcgttta tcattggaga
   109981 agaatttatt ggagatgata gtgtctgctt ggtattaggg gacaatattt actatggtgg
   110041 cggtctgtct aaaatgttgc aacgagcagc ttcaaaagat acaggcgcga cagtatttgg
   110101 ctatcatgtc aatgatccag aacgctttgg tgtagtagaa tttgatgaga atatgcaagc
   110161 tttgtcgatt gaagaaaagc ctgctcagcc aaaatcaaat tacgctgtaa ctggccttta
   110221 tttttatgac aatgaggtaa tttctatcgc taaaggtatc aaaccttcag aacgtggaga
   110281 actagagatt acggatgtca ataaagcata tttggataaa ggaaaactat ccgtagaact
   110341 tatgggacgc ggatttgctt ggcttgatac aggaacacat gaatcattgc ttgaagcatc
   110401 tacctttatc gaaacaattg aaaaacgtca gaacttaaaa gtggcttgtt tggaagaaat
   110461 tgcttaccgt atgggctaca tcgataagga acaattgctg gcccttgcac agccattgaa
   110521 aaaaaatcaa tacggtcaat atttattgaa tttggccgct gaataaggag tccttacaga
   110581 tgaaagtaac agatacaaaa ttaacagatg tcaaaatcgt agaaatggac gtctttggcg
   110641 atcatcgcgg tttcttcaca gaaagttatt caaaagcaaa atttgcagaa catggattga
   110701 aatatgactt tgttcaagac aaccattcac tatcagctga agctggtgtc cttcgggggc
   110761 ttcatttcca aaaaggagaa gctgcacaga caaaattgat tcgggtagtc actggagcag
   110821 ttttagatgt agtagtggat atccgcaaag gtagcccgac ttatggtcaa tgggacggat
   110881 ttatcttatc tgaacataat catcgacagt tgcttgtacc taaaggatat gcccatggat
   110941 tcgttacttt gacaccaaat gtgaacttcc tttataagtg tgataattac tataatgcag
   111001 aagcagatgg gggcatcgca tttgatgatc cagatttagc gattgattgg ccaattgatc
   111061 catcaagagc gattatgtcg gaaaaagatc accgtcatcc gacattaaaa gaatttgaag
   111121 cagaaaatcc atttgtttac ggagaaatct aagaattaag gagaaatttt cttatgaaaa
   111181 acatcattgt taccggaggt gccggattca tcggctctaa tttcgttcat tacgtggtaa
   111241 acaatcatcc agaagtgcat gtcactgttt tggataaact gacttatgca ggaaacaaag
   111301 agaacttggc cggacttcct agcgatcgcg tagaattggt cgtaggagat gttgcagatg
   111361 cagaattagt ggaccgtcta gtaaaagaag cggacgcagt tgttcattac gcggcagaat
   111421 cccataacga taattcatta aaagatcctt tcccatttgt acaaacgaat atcattggta
   111481 cgtatacact tttagaagct tgccgcaagt atgatgtacg ctatcatcac gtatctactg
   111541 acgaggttta cggcgatctt ccattacgtg aagacctgcc aggacatggg gaaggcgaag
   111601 gagaaaaatt cactgcggaa acaccatata atccttcaag tccatattct tctacaaaag
   111661 ctggttcaga tctgttagta aaagcatggg tgcgttcttt tggcttacgt gcaaccattt
   111721 caaactgttc aaacaactat ggtccttatc agcatattga aaaatttatt cctcgccaag
   111781 taacgagtat cttaagcggt attcgtccaa aattatatgg cgaaggaaag aatgttcgtg
   111841 actggatcca tacgaatgat cattcttcag cagtgtggct gattttgacg aaaggcagaa
   111901 ttggcgaaac ctatctgatc ggtgcagacg gtgaagaaga taataagacc gtgatggaat
   111961 tgatcttaga aatgatgggc caaccaaaag acgcatacga ccatgtaaac gaccgcgcag
   112021 ggcacgattt gcgttatgca atcgattcga caaaattgcg agaagagcta ggttggcagc
   112081 ctgagtttac gaacttccgt gatgggttgg ctgacacgat caaatggtac gaagaacatg
   112141 aagactggtg gaaaaaagaa aaagaggccg ttgaagcagc atatgccaaa aatggacaat
   112201 aatacagagg aaatttcact gtattaatgg aaaaacacat gtgaggcagg cgctcgcctg
   112261 tctctttttt aaaataaaaa tcaaggagcg gataaccata tgtttttatt aactggagga
   112321 aatggacaat taggtacaga attgcgccat ttattagatg aaaaaaatct gccatatgtt
   112381 tcaacagatg caaaagactt ggatatcaca gatgctgaga aaacaatggc ttatattaca
   112441 gaattaaaac ccgatgttat tttccattgc gcagcttaca cagcagtaga taaagcagaa
   112501 gaagaagcaa aagaattaga tgagaaaatc aatgtcgatg gcacaagaaa tgtagcactt
   112561 gcagcaaaag ctgtgaatgc acaattcgtc tatatcagta cagactatgt tttcgatgga
   112621 aaaaacaaaa atgtagaata cgaagtagac gaccaaacga atccactaaa tgaatatggt
   112681 cgtacgaaat tattaggcga aaaagcagta gaagagattt tagaagacta ttatatcata
   112741 cgaacttctt gggttttcgg gatctatgga cacaatttcg tcttcacgat gcaacgccta
   112801 gcggaaaccc gcgacaaact gactgtagta aatgaccagt ttgggcgacc aacttggaca
   112861 cgtactttgg cagaatttat ggtatatgtc atcgaagaaa aagcaccatt tggtatttat
   112921 catttgtcga acgaaaatag ctgttcttgg tatgagttcg caaaagaaat attgaaagat
   112981 acagatgtag aagttgcacc tgttacgtca gaagaatttc cgcaaaaagc aacacgtccg
   113041 cagtattcag tcatgagcct gaaaaagaca gaagcgttag gttttgtcat accaacttgg
   113101 caagaagcac ttgcgcaaat gctggaagct gccaaaaaaa tgaataagta atttcctttt
   113161 tatcaaatag aatttggagc atgatatgga aaaacaaaca agaagtgcac gacgaaaaga
   113221 aaagccaaaa gcgaatcagg cagattcgaa tttggtgctt gacctgtttc taaaactccg
   113281 ctatcttatt ggcgttttag tcatcgtggt catcgttact tttaatttaa acggaagttc
   113341 acttggtgtt tgggataaat atgtgtcgca aagagacgat gggaaaaaaa ctgatgtgat
   113401 ttttggacag aatcgggaag tgcggtcaga cgaatggctg gtgcagacac ctttttacct
   113461 ctcacaagct gaagaggact tttcgttggt gaacgaggat tattcattaa acggacaaaa
   113521 catgatcatt gcgtataatt ctcctgtaaa ggatattacg gttatcggaa aaccattcaa
   113581 ctggggattc tttttcctcg gaagagatcg aggactttcc ttttattggg cgatgaaatt
   113641 agtcgtcatg gtcttgcttg gattcgaagt actgatgata ttaacgaaga ggaaaaaagc
   113701 tctatcgcta atcggtgcat ttgcgctgac attttctcca gcagtccagt ggtggttcat
   113761 gcaacatgtg ggcgatttga ttttctttac tttgggactg atgattgctt tttatcatta
   113821 tttctacaat catgataaaa aatggctacg aacgttgatg atgcttttgg tggttatttt
   113881 cggtattggg tttattttag tgatttaccc agcccatcaa gtgatgctgg cgtacttgct
   113941 gattttttat tttgttggcc tgcttattta ctatagcaag aaagtttcat gggatctgtt
   114001 ggatgtagtg ttgattggag gggcagtttt atttattggc ggagtcatgg ttcatttctg
   114061 gctaacgtca aaagacgcat tgttagcttc tttgaatacc ttatatccag gaaaacgggt
   114121 aagtactgga ggaaagtgga cgattggcga atttttttct ttccttacaa attggaaaat
   114181 tccttttgaa gatatcaact attcgaataa ctctgaagtg gccctgttct atcacttttt
   114241 tccaactgta ttcctagcaa gtccatttgt gttatttggc aaaaaagata gcgaacaaaa
   114301 gttactggga cgtattttga tgattttttg tctgttttct atcttttgga ttaccgttgg
   114361 gttgccaaaa tggctagcag aaatcactct gctgtcttat gttcctccat taagagccat
   114421 ccttactttt agttttgcgg cttgtttatt atcgatctgg tttatcgctt atatttggag
   114481 agaagatgtt attcctattt ggtataaatt cattttgttg gcagtaaatg catggtctta
   114541 tttctatgcg attacgcatt cgaaaatgga taattatttt tcggattttg aaaccatcgc
   114601 agttgctgtg ttgggaacag tgattttagc ttttgcatta tttggctgga gacgcttgtt
   114661 ttacctcgct tttgtcgcct tagttatcgt atctggcttt ttcgtcaatc cagtcgtaga
   114721 aggtacagga gcgatttttg aaaaaacact ggcaaaagaa atccaaaaaa tcgatcaaca
   114781 agatcctggt aaagtttggt tgtctgaaga tgaattatat aacttcaccc caacattggg
   114841 agtgaaatcc ttcaatacag tacgctttta tccagatata gctgcatgga agaaaatcga
   114901 tccgaaagaa gaatacgaga agtattataa tcggtatgct catacaagag cttttatcgc
   114961 taaggaagat acaaagttca ttttagatcg tccagatatg ttttcggtca cgttgaattt
   115021 tgaagacgca gaaaaattag ggatcgacta tgtgatcagt aaacgaccat taactgatta
   115081 caaccaatta tatgaagcgc agttcacgca gctatatgga ccggataaag acggttatat
   115141 gatttttaaa gtagaatatc cggattatcc agatgtacca caaacacaaa ctcaggaaaa
   115201 tcagctagtc gtacagtaat aggctagctg agaataatcc tgaaatatta gtagaaagga
   115261 gagtgaagat aattcaagca atcgtacaaa aatttgtcaa caaaatgagt gtcggtttct
   115321 tgcttttgtt gctgacgcta gtcttttttg tttggacagc aggggtaggt tttccagcaa
   115381 ctttgtttat atataacaat agttcattac ttatcgcttt tagtgtggct attttgctca
   115441 ttttaaatgt ttataaagca aaaggcgtcg attttcttta tctaggtgtt gcgcttgccc
   115501 ttttcatttt cttttcttac tttcgggaat taagaaatga gtctttattt ggagacttac
   115561 tgtttccttt ggttatttta ttttgtttag taattaaatg gatcgagtgg acaaaatggg
   115621 atcgcggtat ttatctgatt gtctttacgg tcttcctcgg aatcacgtta tatcgtgtat
   115681 ttacagagat ccaagtacca gaaggacaaa gcatctgggc tccaggcaat tcgctttctg
   115741 atatttggat caatacgaat acaatcggaa gttctttgat gactcttgct ttattgatca
   115801 gtggatttgc cagttctttt gaacgatggt atgttcgaat catcggtggg atagcagtcg
   115861 ttgcagcctt tttaggtatt tgggtctgcc aatctagagg tgcattagtc gcgttgattg
   115921 tttttgcgat attggatctt ttacctaaat cgttgatgcg tgtcattcgt gcgccattta
   115981 ttgcctacac ggttacgatt cttttagctt tgccgatttc ttatctggcg gctgtctctg
   116041 aaaaagtcaa tttgtttacc gatcgagaag atatttggca taaattctat cagacgctag
   116101 gagaaaaatc tgaacaagtt ttactaggaa tgaaaacatt tatcttccag cgaggaaacc
   116161 aatttttagg aaaccacaat agttataatt ctattttgaa tatctatgga ttgataggat
   116221 ttgggattgc tgctttgctg cttatccttt ttattggtcg cttaacgttg aaagcagatt
   116281 tatccaacgg gcagatgact tttatctggg ccttttttgc ggtgatgatg cagtcattta
   116341 tggaggatac attgacttca tacccatggg ttccgattat ttatatcctg ttggctatgg
   116401 ccgttcatcg atatgatgaa ccagaagttg cggaggaatt ctatgttatg gaagaggcat
   116461 atgacgatat gacttatgat gatacatctt atgaggaaca accagatagc gctcattctt
   116521 ccaggatcca acgctatcat tgataggcac aaaatacgag agaggctgtg acaaccgtcg
   116581 cagccttttt tggtttattg caattgtcta gcatcttctg taaaatagaa acgttagaga
   116641 aaatcttgta tcatagaaaa ttaagaaaga aataagaacg cagctcggta ccaaaaatat
   116701 ggtaaaatgg cacttgactg taattcactg aaaatcgctt ggaggaacac aaatgtttaa
   116761 agatttgtat ttatttatga aggacattta tcagaacaga aaattattgc tacagttttc
   116821 gatcaatgac ttcaaatcaa gatatgcagg atcgttttta ggaatcttat gggcgtttat
   116881 caatccgcta tttactgtat tgatttattg gctggtattt ggtgttggac tgaaagcaca
   116941 aatgatcgat gcaaaatacc catttatcgt atatttagtt accgggatga ttccctggtt
   117001 cttcttctca gatgcatttg catcaactac ggttgttttt cgggaatata cttatttagt
   117061 aaagaaagtc gtattcaata ttcgtatctt gcccacgacg aaaatcctat ccaatctgta
   117121 cacccattta ttcttcattc tgattggcat agctgtcgcg cttataaatg gtatatatcc
   117181 ttcagcaatg tctttacagc tgatttatta tttattttgc atgtgtgctt tcttgacagg
   117241 attgacttgg ctgacggcat cgatccaacc atttttacct gatatcatgc aattaattac
   117301 gatcgtgctg cagttgatca tgtggacatt accgatatta tggagcccta atcagtttga
   117361 ttcaggtctt gtaagcatat tgaaattaaa tccgctgtac tatgttgttc aaggttatcg
   117421 tgagtcattt ttaagtgaag cttggttctg ggagcatgga acatatactt tgtatttctg
   117481 ggttttcacg ttagtaatgt tcgtaatcgg ctctgcagtg ttccgtagat tgaaaccaca
   117541 tttttcagat gtgctgtaag gagaatagct atgacagaat atgcaattag actaaaagat
   117601 atcacaaaat cttataatat gtacgcaaaa ccttcggatc gatttcgtga agccctcaat
   117661 ccttttaaga aatcctatca tgatgtattt tatgcgttga aaaacgtgaa tgtagaaata
   117721 aaaaaaggtg agatgatcgg tttcgtggga gaaaacggat cgggaaaatc tacaatgttg
   117781 aaaatcatta cgggagtact gacaccaaca tccggcacga tggaaatcga aggaaaaatc
   117841 tcagctttgc tagaactagg atcaggattc aatcctgagt attctggcta tgaaaatatc
   117901 tatttaaatg gaatggttct aggattttca agagaagaag tcgatgaaat ggtcgatgat
   117961 atcattgaat ttgctgacat cggagaccat atttatcaac ctgtaaaaac ttattcaagc
   118021 gggatgttcg ttcgtctagc ttttgctgta gcaatcaatg tgaatcccga tattttgatt
   118081 gtggatgaag cattggcagt aggcgatctt gaatttcaac taaaatgtat ggaaaaattt
   118141 acagaaatca agaactcggg aaagacgatt ttattcgttt ctcatgatat cagttcgatt
   118201 cgtcgttttt gtgatcgttc ctattggttg aaaaacggag aagtcgtcga atatggcgat
   118261 acgatggaag taacgacgaa ttatgaaaac tttttaaaga aaaaatcagt taaaacagtg
   118321 gatcggaata aaaaccaagt agaacggttc gccgctccag atatcgtcga cgtgacgaaa
   118381 gcggaattgt tggataaaga caaaaatccg gtggatatgc tggaacaagg agaaaaacta
   118441 tacgttaaag taacttatga agtaaaagat gatacaatca aaaatccggt tttaggaatt
   118501 gcgctacgaa cagtcgacaa tttttatgta tgcggactta atactctact agatggtatc
   118561 aagattcctt ggaaaaaagg agagaatatc ttttatctgg agtatgaaaa gttaggtttg
   118621 ttagctggtg aatattattt tgacgttgcg gtctttgaag aaaatgcaac ggttcctttg
   118681 gtatataaga caaagtacat gaacttgttt gtcagcggtt cttatatcgg agaaggcatc
   118741 gtggtcttag accataaatg ggaagaaggg actcatagca atgaaatatg attttgaaat
   118801 ggacctagat gaacaaagca gtgtgggaaa aattgccgca cagatcaaac caggaagtaa
   118861 agttttagag tttggtcccg gaaacggccg attaacgaaa catttaatcg gagcaaaaca
   118921 atgtgaagtg agcattgttg agttagataa ggaattgttc gattttgtca gcgaatttgc
   118981 tcaagatgga ttttatggtg atatcgaaag ttttgagtgg gcaaactatt atgctggaca
   119041 gacatttgat tacgtcttgt ttgcggatgt tttagaacat ttggtagatc ctggaaaaac
   119101 attgaagaaa gtcagagaat ttttgaatga agaaggagag atcctgatta cattccctaa
   119161 cttggcgcat aattctgtca tgatcgattt gttcaataac caattgccgt gggcttctta
   119221 tggtctccta gatgaaaccc ataattcttt ttatactcat gatggcttcc aaaaagtttt
   119281 tgaaaaagcg ggattattca ttaatattga agattatttg tacttagctg taggtgatac
   119341 agaattaaaa tcaacttatg aagaattgcc ggaagctgta cgctatgatt tcaaaatgcg
   119401 accatttggt gaagtctacc aatacttttt ctcattgatg aaacatccag tagcacaaag
   119461 ttccattgca gagccacaaa attctaatta tgtaaaagtg ttagaagtaa cccagcaaac
   119521 gaaacaagat gaaacaatcc aacaaatacc attcaataat ttcacagggg aaaatgagac
   119581 actaagtttc cccgtatcag aaacaacaga aagaatggtg tttcgcttcg cgcaacaacc
   119641 tagctttatc gaattcagcg cagaagctgc cggagaaaaa ctaacattta tcgattcgaa
   119701 tgctgtagtc aaaacagtaa atgattgtta tttgtttgat gggaaagaat taccagagtt
   119761 cgttctaacg gatatttctg ggaaagaagt gactatccat tgtcactatc gctttatcgg
   119821 ggaattgaca ccaacaatga aagaattgtt ggaaacgatt cgtcccatgg ctgaagtcac
   119881 aaagcagctt tctgagaaaa atgacgaatt agaaagagaa aatcatcatc ttcttgagga
   119941 aaatacaaga ctagaccata cgttaaaaac aaccacaaat cgctattgca cattgttgga
   120001 aagtgatgaa tggacaataa aacatagact aggacgacgt aagaaagaaa cttcgaaaaa
   120061 aatccaagaa aaagaacttt ctatatgtat tgacgagaaa atctgggatg cagaaacaaa
   120121 aatattaaaa atcatcggat ggggaatcgc taattcggat cgccagccct tgtcctataa
   120181 gttatcagct gaccaatcgc catttttcga agcaattcct gtttcacgag aagaagtcaa
   120241 tcaagcagaa cagctcgcaa aaggaacaga agcgggattt gagttacgaa tcctttgcga
   120301 gcaagaacgt tcattcttag tggaagcagt agctcaaaat ggccaatcat ggatcattga
   120361 aatgtaaata atgattaact tataaagata aggagtaaac gaatcgtgca aaaaaaagaa
   120421 attgccgtat ttatcgatca aatcactcgt gaacgggcca ctggcgattt gcttattgtg
   120481 ggctgggcaa ttgatgaagt gaccaaagaa atccctacga taaaggtaga aaaagaaaat
   120541 gtgattgctg aagcaacaca tgtcgtaaga ttggatatca atcatttata taatcttgat
   120601 gttaagaccc aaagcggttt caaggtacgt ttatcaggga aaatgagagg aaaagccatc
   120661 ttagacttcc aaacagcgag acatcaaaat ggcatagctg tgaaattaaa cggtaaatac
   120721 ccttatgatg acggcattga atcttcatgg gaaagaaaaa aaagattact gaaaaaaggg
   120781 ataaattatg caaggactca tggcgtaaaa aaagcgatcc gtcgggtcaa attggaattg
   120841 aagccaggat cgatcgacta tgcagagtgg atcagtcgtc atgagaaacc agatttgaaa
   120901 gagcaaagga aagaagcaca aggatttgcg taccgtccat tgatttccat acttatgcct
   120961 gtctataacg ttgaaatcaa atggctcgaa aaatgcattg attcggtact ggatcaaacg
   121021 tatgatcatt gggaactatg tatcagtgat gatgcttcta ctgatccagc tatccgaaag
   121081 tgtctggaat cctatcaagc aaaagatgat agaatcaaag tcgttttccg aaaagaaaat
   121141 ggacacatca gtctggcaac taattctgca ttggaaatag cagaaggaga atttatagca
   121201 ctcttagata atgatgatga attaccgcca tttgcattat atgaagtggc aaaagtgcta
   121261 aacgtacatc cagaactgga tgtgatctac agtgacgaag ataaaatcga tgcagatggc
   121321 aatcgctttg atccgcattt caaagcagac tggtcgcctg atacattgat gggaaataat
   121381 tatatctctc acttaggcgt atatcgaaca agcattgtta aagaacttgg cggtttccgt
   121441 aaaggctatg aaggttctca agattatgat ttggtccttc gtgtgacaga acaaatccct
   121501 gcagatcata tttaccacat tgaccgagtc ttgtatcatt ggcggacgat cccaggatca
   121561 actgcaagta atggcgaagc caaaagttat atctatgatt ccggagtgaa agcattgaca
   121621 gatgccctgt ctagaagaaa catcaaagga agcgtacgtc ctggcagaat ttctggattt
   121681 tatgagattg cttatgacgt cttgcaagag gatttggtca gtgtgatcat tccaacgaag
   121741 aatggctatg aagatctaaa aacttgtgtg gattcgatca tcgaaaaaac gtcttattca
   121801 aattatgaaa tcattattgc ggataacgga agtacagatc caaaaatgca agaattattt
   121861 gcagaataca agcatcagct gaaagaccgc tttatcgtag aattgattga cattccattc
   121921 aattattctc gaattaacaa tttagcagca gaaaaagcaa acggcaaata ctttttattc
   121981 ttgaataacg atacagaagt gattgagcca gattggatga cggtgatggt ttcttatgca
   122041 cagtttgatc gaatcggctg tgtaggagca aaactgtttt atccagatga tacaacgcag
   122101 catgcaggag tattattagg aatcggtggt gttgcagggc atgcattgaa taactatgat
   122161 cgaacacatt gtggttattt cggccgattg gtcattgacg tcaattactt agcagtcact
   122221 gcagcttgta tgatggtcaa ggcagctgac ttcaatgctg ttaacgggtt tgatgaaaca
   122281 ttagaagtag cattcaacga tgttgacttg tgcttgaaag tttatgaact gggtcgttac
   122341 aatgtctatg cacatcaagc agaattgtac catttcgaat ctaaatcaag aggttatgaa
   122401 gatactccag aaaaacaaag acgttttgct ggtgaaatca aaaaaatgca agataaatgg
   122461 ccagcatata ttgcgcatga tccattttac aatgataatt taacaaaaga cggaatcggc
   122521 gacttttcgt tgagaccaga ttaaactata ataaaaagct gttatgttgt gattcgctcg
   122581 cacataacgg ctttttatag ttttttgtca aaaaaaggca gaaggtgccg actcaaaaaa
   122641 ataagtcgag gttgggacag aagtgtttaa ctccgagaaa taagaagaaa ttcacgaaaa
   122701 ttgcttttca aatttttgtg aatttcggct tatttaagaa gaagttgctt ctgctcccac
   122761 cgtttattcg tttttaaaga atgagacaaa agtgtttctt acttttgtcc cacgctctta
   122821 ttcatgctca gagcgccaat agatacaaaa tcatagccaa agtttcacga accctagctg
   122881 tcaaagaatc gatattttat tttaatatag gctattttaa aataaatggt ttaaggtatt
   122941 cctctacaaa agccactcta tcaattatca tgggtttaaa ataagaagtg atggagataa
   123001 ccagattgtt cttagcgtca atataaagaa catttccact attaccaatt gctgaatata
   123061 tttcgttctc agaatctaat acccaccata aatagccata tctcatattt tgtaagtctt
   123121 ctaaagcact ggttctaggt ttgaacatct cgtttatcca catgggtggt acgatttgtt
   123181 ttcctttata catcccttta tttaggcata atacaccaat ttttgccaga tcacttgccg
   123241 ataaacacaa accatatcct gccgtcccga tatttagtgg atcacaaaac cagacattgg
   123301 ttttaggaga cttatccatt gtaaactgct tatgttcttc agctgtttca gcataataat
   123361 tgacatggtt tggtatccct ataggatcaa atagatactt attggcaaaa tcaacagttg
   123421 ttagcttact tgtctgatac aacaagccag ttaatatgtg taaacagaca gtttgatagt
   123481 tgaattcgcc ggttatacct tttcttcctc ccaataaatc tagtgatgtt tttgtccaat
   123541 cttcacttga acagactttt gtccatggat cgcctttaca tttgtagggt gctttcattg
   123601 ttagcaagtg cttgattgta atagcgtaaa tagttttctc cccgcgtttc actttatact
   123661 ctggaaaata atctaatact ttatcatcga tacttttgat taatccttgc tctaaagcaa
   123721 tgccaatcag caaagataca atacttttcg ttgctgacat gatatgacaa gtatcgtctt
   123781 ttttataacc attccattca tctgtataaa cttctacccc atccttatat gcgacaattt
   123841 gacatatatt tggttgtcga aaaccaataa aatcatgcaa ctcttgttta ttcataagat
   123901 attacctcac tttcttctat aagcataatg tcattaaaaa tcgatttcaa ttttttcgat
   123961 tcttcacgta atacaggtat tcttatggac tagacagcgc tttgggaaga tggattctct
   124021 gaaaaatata aaggttcact ttatgagtgg ataaaaaaag cagcaaatac ttcgacttct
   124081 ttaatttacc aacattagtg aaaatcgatt catctggaga tttgtaattt agagtccttg
   124141 tcttgattag aggtgtataa agctttttat tagacatttt taaattccat tacaatttgc
   124201 ttataaacta ggtttttaaa ggtgactatg gtaagataat agagatatta ttttgtgatt
   124261 aggagtgtat atatgaataa aaatggagaa tggagtgctg cacgacggat attcattata
   124321 ttaatggatg ttattgtata caatgcagca atatatagta gtttcttact gaagtttcgt
   124381 ggagagatcc cattacgaaa tttagaaact ttccaacact cagcaattat tatttcagtg
   124441 atttttattg cgttgaacat tttgttgggt gcatatgttt tttataatcg aatgatcagt
   124501 gacatcgtgt ttgtcacagt catcggtcag gtattgatga cactagggat catggtagtg
   124561 acttttgcgg gccgctggtt tgcttttcca cgtcttgtta tcttactttc atttgttctc
   124621 ggtacgcttt tcttaagtat ctatcgtatt ctcgtatata aactttattt acgcgtaagt
   124681 cgagataaaa aggtcgtcat tattgggtta gaaaaggatg tcgcaccagc aatccaaaat
   124741 tttggttcaa agaaaaacaa caaacataaa gtcgaagcag tcattcttga taactattat
   124801 gcaaacatca aaaatatgat taaagacatc gatattgtct atcttgcttc tcatatcgaa
   124861 gaaactgaaa aaatcaaaat ctatcaatta gttactaaaa atgaaaaaaa attgtttttg
   124921 aatacaacat ttgaaaactt gacattgatc aatccaaata ttatgaattt cgaagatgaa
   124981 agtattattg aagcatctgg atttagaatc caacctgaat atgaactatt caaacgtttg
   125041 tttgatatca tcgtttcact tgttttatta gttattgctt caccatttat gttattcaca
   125101 gcaattttag tcaaagtgac atcaccggga ccaatcatct ataaacaagt gcggatcaca
   125161 aaaaatcaaa aggaattttc aatctataaa ttccgctcga tgaccgccac agccgaagca
   125221 aaatctggac cagttttagc taagagcaat gatgcacgag taacgcctgt aggtaaattt
   125281 attcgagctg tccgctttga tgaattacca caaatcttca atgtgctgaa aggcgatatg
   125341 tcgatcgtag gaccacgtcc tgaacgttct ttcttcgtcg atcagttcaa tgcagaaaac
   125401 ccttattact acttacggca taatgttcgt gctggtatca ctgggtatgc acaagtctat
   125461 ggaaaatatg tctctgatta caacagtaaa ttacgttttg atttacttta tatcaaaaaa
   125521 tattcattga tgcttgattt aaagatcttg ctgcaaacca tcaagatcct atttgataaa
   125581 atgtcctctc aaggacttga agaagacgaa aatgtgatct cattggcaga aattgctttt
   125641 ccgaatgaaa aaatctttcg ctagttggga ggcgtaaaaa tgaacaatcc gaaattcagc
   125701 ataatcgtac cagtatacaa tgcagaagca actattggtc gaacgattga tattctaaaa
   125761 gaattggatt atgaagagta cgaagtcgtt ttagtaaatg atggatcaac agatcaaaca
   125821 caggaaatca ttcaagaact gattgaaaat gatgaacgtt ttgaattgat tactacgacc
   125881 aatcaagggc ccggattagc aagaaacgaa ggaataagac agtcaaaagg agaatatctc
   125941 ttattcttcg atgcagatga cacgccagta aaaacgatct tgtcagatta tgatcatttg
   126001 ctagaaaaga gtccaaaagc ggatcttgtt gttagttcgt ttgtttttcg tacaatggac
   126061 gagggaagaa tcgtttccga aaaagaaaac ttagttacag aatgtcacta tctaactcac
   126121 gaagagttta taaaagacat gtatcagtta atgaatgaac agttgatgta tgttgtatgg
   126181 aacaaatgct atcgtagcga tattctgaaa aataaccaaa ttctatttaa aacttataat
   126241 agttgtgagg atcgaatatt taacttacat tattatgaat actgtcatca agtgatcatg
   126301 aacccaaaag tagaatatat ttatgaattt gaaggtggga aggggatcac taatcaatac
   126361 cgacctaaca agttcgaaac atttaaagaa ttctattcat tagcaaatac tgtaacagac
   126421 gatattaata aagacggtat ggcggcgctt cttttaaaag ggacaacttc agttattttt
   126481 tctatttata gtacctctgc aagaacttca aaagagaaga aagacgaagc aaagcagata
   126541 ttgagtgatc cagtgatccg agaagccaag caaattgcta agacggattc ttcagcaaaa
   126601 aaaataacaa agttgcttta caatatgcca gctccactat ttttatctgc tgtaaaagtt
   126661 ggaagcttcg ttgaagtgaa aatgccaggg ttaatggctg ctttgaagcg agtatattag
   126721 ttaatggagg aaaatataat gaaaagaatt ttgcattttc agggacgaat gggtttaggt
   126781 ggagcagagt ctttcatgat gaatttatat cgtaaaattg atcgtacaaa ctatcaattt
   126841 gattttttga tttatgaaga ttatgcagat gttcaagact atcattcaga gattgaaaga
   126901 ttaggaggcc gtatttttgt tgtacctaat cctaagaaaa atatttttaa atatttaata
   126961 gaagtgaata agttactaaa aaaagaatct ttttctatag ttcacaatca ggtttatttt
   127021 ggcgggggaa ttaatttatg gttagccaaa aagaatggaa tcagacagag aattgctcat
   127081 agccatgcaa ctgaagatgg aaaatctcaa aatattgtaa tgaatgtatt gcgtaaattt
   127141 ttaaccaaat tgttgttaca aaatgcaaca gactaccttg ccgttagtca agaagctggt
   127201 gaaagtctgt ttcaaaacca tccctttgag atagttcata atggtattga tttagagctt
   127261 tatagtaaaa attcagaagc taaggttaat aagagaaaag aattggatat ctcaatgagt
   127321 acttttgttg tgggaaacat tggacgatta gagaaacaaa aaaatcaatc ttatttgtta
   127381 gagatttttt tagaaatact aaaaatgaat tctgattctt acttgatttt agtaggtgaa
   127441 gggtcactta gaaataaact gaaagccaaa gcagtagaat tggatataga gaaaagagta
   127501 aaatttttag gagaaagaaa cgatattccc gagttattat ctgtgatgga tgtttttgta
   127561 atgccttctc tttatgaagg tctgccaatg gttggattag aagcacaagc agcaaaatta
   127621 aaattagtct tagcagatac tatttccaaa gatacaaagt taacaaataa tgtggatttt
   127681 gtatcattag atgaaacacc acaagaatgg gcaaaaaaag taataaaaga gccgtatgct
   127741 aataaaataa cacaagaact aaagagttat gatgtttcgt acactactga acaaatggag
   127801 agtatatata gtagagatca ttaaaaatgt tcaagatatt ttaggagtga ttatttttga
   127861 aaatatgtta tttgatattg gcatacaatg atattggcaa cttaaagaga ttggtcaatc
   127921 gattgaacga aaatgcagtt tttattattc atatagataa aaaaactgat atagaaccat
   127981 ttattgaagc atttaaatca tatgataatg ttgaattttt atgtaataaa aaccgtgtta
   128041 aaatttattg gggtggtttt tccattgtac aagcagaaat caatttagta aagagagcat
   128101 tacaaaatga gaaatattta aaatatgtat tattatctgg tgctgattat ccaatcaaag
   128161 ataatgagta tatttataat tattttaaaa agaatagttc agtggagttt attcgtggaa
   128221 tagatctaga tcaaattaaa cataaagaat tttattataa acatatagat gtatatcaaa
   128281 aacatgatta tcctagaatt aataggaata acacaactgc atttaaaatt tttagagcta
   128341 taattaatcg ttgtctgagg atgattaaac ttcccccaaa aattagacac cataaatttg
   128401 atttgtatca tggttctcaa tggtgggctt tgtcgaaaga atgcctaaca gaat
//
LOCUS       DARBET010000003        89653 bp    DNA     linear   BCT 07-NOV-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4027
            SAMEA114569841-rid20112183.denovo.003, whole genome shotgun
            sequence.
ACCESSION   DARBET010000003 DARBET010000000
VERSION     DARBET010000003.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA114569841
            Sequence Read Archive: ERR12203666
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 89653)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 89653)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001972618.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001972618.1
            Genome Coverage       :: 132x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/07/2023 13:18:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,041
            CDSs (total)                      :: 2,975
            Genes (coding)                    :: 2,908
            CDSs (with protein)               :: 2,908
            Genes (RNA)                       :: 66
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 67
            CDSs (without protein)            :: 67
            Pseudo Genes (ambiguous residues) :: 0 of 67
            Pseudo Genes (frameshifted)       :: 26 of 67
            Pseudo Genes (incomplete)         :: 28 of 67
            Pseudo Genes (internal stop)      :: 20 of 67
            Pseudo Genes (multiple problems)  :: 6 of 67
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..89653
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA114569841-rid20112183.denovo.003"
                     /isolate="VRE4027"
                     /db_xref="taxon:1352"
                     /country="Denmark"
     gene            complement(552..2165)
                     /locus_tag="SCJ86_000238"
     CDS             complement(552..2165)
                     /locus_tag="SCJ86_000238"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HEG1167832.1"
                     /translation="MKVVVIGGVAGGPSFATRFRRLNEAHEIIIYERGENISYASCAL
                     PYYLGGVITDRDSLIERTPEILKTKNNIDVFTKHEVTAIDPSTKRLTVKDLSTNEEIK
                     TDYDKLIISSGARPDYPDIPGVFEAENGFVLRSVTDADRIKSFLEEKNPQHVVILGAG
                     VMGLELAENFKHRGLDVTLIDQLPQVAFPYDPEIANLVYDKLLKEGLAVHLETRVTEI
                     RDKGREIVLSDGSVLSADMLIFAVGVSPKNEVVKATGIQLSDTGQIIVDDQLQTNLPD
                     IYAIGDIIETTSVVTGQPIQSMLSSAANRQGHMLADILNGTPMRYRGYIGAGVAKIFD
                     HTASYAGMTEHELKASGITNYKTVFITPFDHAYFYPEATRLNLKLIFDADSGRILGGQ
                     AFGEKGVDKRMGELSVAITGNLTVFDLPDLELPYSPPYSTTRDPLNIAGYVAINQMTN
                     IVETIKASDIPENDLKEAFFLDIREPNKAPSGSISATKNIPMNELRDRINEIPKDKKV
                     YITFRRGLNTYTSARILAGLGIKAILIEE"
     gene            complement(2306..3214)
                     /locus_tag="SCJ86_000239"
     CDS             complement(2306..3214)
                     /locus_tag="SCJ86_000239"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296511.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HEG1167833.1"
                     /translation="MVLLLTNNQSSNLVKKTLTYSIDTHLHLSAAPYFRLLINESANL
                     YAEFAHKSIELDNPSLLFIDKQQEVQIFSTQKETQTFIIIQFYFDEQLLAHITGQSFS
                     IDQEITSIITFLKNEQFKIDYLKHSKPAISYDQEINSFFILCQQMSIHLLSLLVTYCL
                     QKIQLSSLPRHYQQELALLTIPSNEKVIPKKKHILYSDELVDPIIRYLEEHLSENFLL
                     EQLAQIFFISPASLKKHFRKVNGKSVISYFKELKMEKAKRLVLENELTYTEIADILGF
                     HSIHHFSSAFKKYTGLSPSKYYLSLK"
     gene            3397..4521
                     /locus_tag="SCJ86_000240"
     CDS             3397..4521
                     /locus_tag="SCJ86_000240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287757.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HEG1167834.1"
                     /translation="MKKRYKLVIALLNITLLFSLSACGKTNEEVTANIADPMIKKATT
                     LEVYVANGKLTNYVENAAKIYNRKTGANIDLKIVNVASGTATVQMITPKLVTHEDMPD
                     IISMADTSAAGVLEKFQDAFYSADEYGFYDKYGDQFYDQKLNILRQQTSNETIIPWAS
                     DFTPALCYYQPKKFAEIGVDFEEISSWEEYIQVAKALKEKTGSFGIALPESGDQELFI
                     NMLAQQNQSLLDEEGNIRLNTKEAKHAAKLIKEMIDSDIVHFYGAQDAEKAFQESAMF
                     VAGGWYATNMSLNFPDASDKWRMAPLIPFSKENPGKGAVSGGSSFYVPKDAKNPQVAQ
                     QFLTFMLTDEECLANALELGVATSNSLAYQTEAAEKKFDY"
     gene            4748..5665
                     /locus_tag="SCJ86_000241"
     CDS             4748..5665
                     /locus_tag="SCJ86_000241"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002351119.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HEG1167835.1"
                     /translation="MMQSSLPSKTIKNSKKKATDTSHKKSAYGLVTPFVIFFIVTILI
                     PIIISFAFSFMKIGYSFEFVGFDNYLAIFRDPLFIKSYTNVLIIMAGSIPITMLLAIY
                     FAVLLNSAQLKGKGFFRTVYYIPTITSTVAVASVFMTFFNPTGLFNGFLNALGINGVQ
                     WLTNPFWIRISMIITMIWLSVGYNTILFLAGLQGISKEIYESAEIDGASKFRQFFLLT
                     IPMLKPIILMATVLATINGLGNFNIPNIFFGTSNGPENSALVVGVNLYKTSFEMVDFG
                     KASAIAWTMVFVSVILSAIQFKFGGDKND"
     gene            5658..6491
                     /locus_tag="SCJ86_000242"
     CDS             5658..6491
                     /locus_tag="SCJ86_000242"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287754.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HEG1167836.1"
                     /translation="MTKQKIQSRIFTYVIIVFGALIFLMPYLYMIVASTQSNQEILSG
                     GINFKFGDMLLKNWQAIMARYNYPLIIWNSIVIAVISTVLATASSTMAGYALAKYRFM
                     GNKLLFNTVMLSRMVPFFTTLIPLFYIMSRLGLSNTYAGVIIPSIASTTSVFMMRQYS
                     SQFPTALMEAARIDGANEWVIFTKIAVPILSPTIITSGLLIFMGSWNQYLFPLVMLSD
                     KDKYTVPLIIKNMTVSSTGEPINYGAIMLILATSVIPMVLIYVWAQSKFKENDIGAAN
                     K"
     gene            6574..7692
                     /locus_tag="SCJ86_000243"
     CDS             6574..7692
                     /locus_tag="SCJ86_000243"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002354443.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 88 protein"
                     /protein_id="HEG1167837.1"
                     /translation="MNEQTKEWSRKILNVASEKIDAELDRMGNKIPYISYNGKYTDVI
                     KDRGIDWWTNGFWSGILWQLFHKTKREKFKEAAIQQEQLLESALYHFSDVHHDVGFLF
                     LPSAIAHYQQSQNKHSRQLGLHAATVLAGRYNISGNYLVAWNDHKPGWMIIDSMMNLP
                     LLYWASRETGDPRFSKIARQHADTTAKYLVRKDGSTGHIAEFDAESGKYIRLLGGQGY
                     NENSCWSRGNAWALYGFALSYRYTKNPYYLAIAEKIAVNFIQKIEKTGYIPLIDFAVK
                     DSNIDTSAGVCAACGLLELAGYVSSVRSKMYHEAAINILQKTEKHYANWNMDEDGIIG
                     GGAEAYHRELTYNVPLIYSDYFFIEGLLRLEQSELAIW"
     gene            7686..9584
                     /locus_tag="SCJ86_000244"
     CDS             7686..9584
                     /locus_tag="SCJ86_000244"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321415.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2264 domain-containing protein"
                     /protein_id="HEG1167838.1"
                     /translation="MVVKRGENTMKHPVPLLRTRQDYFDLFKKINQPLQPFYRASGAQ
                     IDYIHQGVGYGNKIAGMEGFSRVLWGAGPAIDQLDERWLKLILSGIKAGTDPSHPDYW
                     GEIHERDQRMVEMPAMLLALYHHEQLLWKKLDTGEKSQIIHWFSQIFEYECADGNWQF
                     FKIIVGTIIQKFGYPVKESALKEAFDNIENCYLQNGWYRDSSRGRQDYYNPFAFHYYG
                     LIYSVLEPEKPISQIYKNRAIDFSRDYIHFFAEDGANVPFGRSMIYRFAVSSFWSAML
                     WADLLPFKLGEIKGLVNRNIRWWMQKEIFDEKGILNIGYTYPQLTLTEPYNSTLSPLW
                     LNKIFLLLALPEDHEYWTAKEMPMPIREGTKLLSEANLLIQHDNGHTFFLNAGQLGPN
                     FHTLTNEKYLKFAYSSQFGFSIPRANQLKAEMAMDSMLGIQRADTYITTSFKNESIKT
                     YGQFIVRNNVRDIVCKKEVLASTWEPTLQAKIRTWLIPFEGWQIRVHKVELDTEYVLY
                     ETGFAIECSPEKEGIIIEEDKENYRVSEAGFSGIICLSDDTIKRKSTAIMGLPNTNLM
                     TWENTFIPGLEGTFGKGTHWLGTGVVAHQDRDYSLEVWNNRPKIDFDGTTGLTIQNKN
                     NKKRLKLC"
     gene            9578..10801
                     /locus_tag="SCJ86_000245"
     CDS             9578..10801
                     /locus_tag="SCJ86_000245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300757.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 88 protein"
                     /protein_id="HEG1167839.1"
                     /translation="MLKAGGEMLDKVMFEEIIHPEKWINECYKELMEKEWNNEIERCR
                     RHTERLIKRISNSQYQFPSECPIKGRYPLRDNIGWTEGFWTGINWLLFEKTGDPLFQQ
                     TAEKADIDFIHRIDCQIITNHHDLGFLYSLSTVADYKITGNRKMKEASIDAAETLLQR
                     FWEKAGVIQAWGDPSDPSQQGRMIMDCCMNLPLLYWASEETGNQKYKKAAQAHIQNAA
                     DYLVREDGSTYHTFYMDVKDGKPRYGSTHQGYSDNSCWARGQAWGIYGFALSYRYLKE
                     QKYFDLSKILANYFMNRLPEDTICYWDLIFDDSSREEKDTSSMVIMVCGLLELIESGL
                     LDKTEKNLYQSAVWKIMRQLKERYATTEEEDGFLTQSVYNKPKNNGVNTPCIWGDYYY
                     LEALVRMTSNWSSYW"
     gene            complement(10887..12821)
                     /locus_tag="SCJ86_000246"
     CDS             complement(10887..12821)
                     /locus_tag="SCJ86_000246"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F family ATP-binding cassette
                     domain-containing protein"
                     /protein_id="HEG1167840.1"
                     /translation="MILLQANQIARHFGAEVLFENIHLEISTGARVALVGRNGAGKST
                     LLKIIAGIEAPDEGKIAKNKTATLGYLAQDTGLDSNETVWNEMLKAFEQVRQMEERMR
                     ELEIFISEGTPDTPAYSSYLKEYDRLQHDFSDRNGYGYENEIRSVLHGFKFDSSFYDQ
                     KIDTLSGGQRTRLALARMLLQHPDILILDEPTNHLDIETLSWLENYLQSYTGALLIVS
                     HDRYFLDKVVNEVYELSRRKMRHYKGNYSKYLDLKAEQLASEWKAYEKQQTEIHKLED
                     FVARNLVRASTTKRAQSRRKTLEKMDRLDRPQGDEKQANFLFSIEKPSGNVVLQIEDA
                     AIGYDNVTLSEPINLDIRRQEAIALVGPNGIGKSTLLKSIIHQIPFIRGKETLGTNVT
                     IGYYDQGQADLHTNKTILAELWDEHPTTPEKEIRSALGAFLFSGEDVEKPIPLLSGGE
                     KARVALAKLAMNKENFLILDEPTNHLDIDNKEVLENALIDYDGTLLFVSHDRYFINRI
                     ATKVVELSENGSKVYLGDYDYYLEKKQEEEEIAELLAQDEPESAPAPTNKSDYYQSKE
                     QQKLIRSLQRKITQVEEEMARIDEWIDQLEQEMVAPENLEDHVKLNELNQDLEAARQD
                     QETKLTEWEELSLELEEYEN"
     gene            13047..13703
                     /locus_tag="SCJ86_000247"
     CDS             13047..13703
                     /locus_tag="SCJ86_000247"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287746.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="redox-sensing transcriptional repressor Rex"
                     /protein_id="HEG1167841.1"
                     /translation="MKDHIIPKATAKRLPLYYRYLRILNNAGKKKVSSTELSEAVQVD
                     SATIRRDFSYFGELGKRGYGYDVEDLMHFFAKTLNDDQLTNVALIGVGNLGSALLKYK
                     FHQSNSIRISCAFDVNEEIVGRIVDGIPVYPMEDMSEQIKIQQIEVAILTIPAERAQE
                     VVNQLTEAGIKGILNFTAARLNAPSDVLIQNVDLTNELQTLIYFLHSDANAHSENTAE
                     "
     gene            complement(13777..14130)
                     /locus_tag="SCJ86_000248"
     CDS             complement(13777..14130)
                     /locus_tag="SCJ86_000248"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317922.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin family protein"
                     /protein_id="HEG1167842.1"
                     /translation="MKQTTNLTEVQDILQQSSVAVIYLSMPNCSVCHAVRPRLEELLT
                     HYDIPALHLDAFETPEVASKFEVLTAPVVLIFHQGKEVFRQARFIDFKKIRYLLDELT
                     AEDDSLSYEEIFRTK"
     gene            14322..15251
                     /locus_tag="SCJ86_000249"
     CDS             14322..15251
                     /locus_tag="SCJ86_000249"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306129.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="permease"
                     /protein_id="HEG1167843.1"
                     /translation="MFSFIPNSVLQMSTIFLSIVIEALPFVLLGCLISGALQVFLTPE
                     RVKRWLPENRIFSIFTGSILGFFFPSCECGIVPIVHQFVKKGVPVHTAFAFMLTAPII
                     NPIVIFSTFIAFGNSWQMASWRVIGSFFVALVIGVWLAYFQKDAVLKEKITSKVAENH
                     HHHVHEEEQSEKNEPKNQLIHQTGHVLTHSIDEFFDTGRYLIIGGLIAAAMQTYLPTR
                     IMLTLGSTKILAIIIMLLLAFTMSLCSEADAFIGSSLLSLFGTAPVAAFLVFGPMVDI
                     KNLLMMKRYFTGRFILSLIGLIALSVIGFTLFI"
     gene            15262..16098
                     /locus_tag="SCJ86_000250"
     CDS             15262..16098
                     /locus_tag="SCJ86_000250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287743.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR03943 family protein"
                     /protein_id="HEG1167844.1"
                     /translation="MLRFLILSGYFELMMYLQVSGKLDQYINVHYRYLAILSMILSAL
                     LALVQLYLWVKNGKAEEKAHEHDHDHGLSKPYQRGIAYVLLALPVIVGTLFPTVSLDT
                     TIVEAKGFNFPVSKESVGDPEMQTQYLKPDTSIYFNKSDYLDQMNELKEKYGDKQTIK
                     ITDENYLEVMELIYNYPSEFIGKKISYEGFVYQTPNDSNTDIFLFRFGIIHCVADSGV
                     FGLLTHMPEGVTVKNDEWYKIEGTIQSDYYQPFKREIPSVVVTNAEKVSAPKNQYVYR
                     SF"
     gene            16346..17122
                     /locus_tag="SCJ86_000251"
     CDS             16346..17122
                     /locus_tag="SCJ86_000251"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HEG1167845.1"
                     /translation="MVTRIFILDDSLVFEKMLVEILEESRILLSPWKFEIVQGLNVIQ
                     FVEFIKNNEIYSSDIFFLDIDLNAYYSGIELGEMLREKNEKCKIVYLTSHPNKAITVI
                     NRKITPSGYLIKEFTYEKNKKSIQSYLLKEKENYPVQHATKKRWTTVRYGNSDHYIHY
                     DEILYLMSMPGYKNKIGLVLKDEEILVNGTIKKYKKSLEYEYLCKDLKAYIINTENIT
                     SISRVNEIVTFANGEQLEMGKRSIDKLKNFMQEKIENESF"
     gene            17298..18041
                     /locus_tag="SCJ86_000252"
     CDS             17298..18041
                     /locus_tag="SCJ86_000252"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300761.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEG1167846.1"
                     /translation="MIEFINVSKSYGKKQALKDLSLSIRQGEIFGFLGHNGAGKSTTI
                     KSLVSIIEPSSGTILVDGMKLTENRLSIKQKIGYVPDSPDIFLQLTAGEYWDLISAAY
                     ELDTQKKEKRLAELTALFDMYSHQNETIASFSHGMRQKTILIGTLLPDPDIWVLDEPL
                     QGLDPQAAFDLKEMMKAHAAKGKTVIFSTHVLDTAQQLCDKLAILKKGELIYQGAVSE
                     LLNGSPDETLEKIYLKMAGRQATGDGPYV"
     gene            18034..19614
                     /locus_tag="SCJ86_000253"
     CDS             18034..19614
                     /locus_tag="SCJ86_000253"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter"
                     /protein_id="HEG1167847.1"
                     /translation="MYKRQLLELIRVNLLYVNPQLTNKAREKGKKGKRLTIYLLSQNL
                     FSGLIFLLIYGFAMFAMNFVKLPGFFTYYVALFGMLGLTQSISVIYNIFFEGNDLQVF
                     LPLPFGQEQVFSAKILVVALTIIPFVFPLLVMFILTGWRADLGILLTIIISILLFSLF
                     LIILFCLASLIVFGLARTAVFKKHRKTVSSILLGSSMLIAFAGIMWMNHQTGTIENEL
                     TDRHPISILLPFYDAASQPLSQQGLLGWGILAITALGFFLAVKQLIVPKLTEQLTDSN
                     SEFKIKRTHKKNQNVHQLLFSYNAQLVKEPNLIMQVLSSSLLTPIIFILAFALGGEIK
                     LTDLDNKLIGVVFLVGIALAFLTVNQTSFISNLISLDQENFLFIRSLPISMNQYLKEK
                     FRFGWILQSFLTGGIALLSGLSFQLPLFFIPSLLFGSLFGCYLLSLRYFARDYRLLLL
                     NWTNINQLFNRGSGSLGLVATMMGSLIISIILIVLYGMLALFYPFWLVNIPVIGLVLI
                     LSALWIVYYQRTFWKKFE"
     gene            complement(19865..20344)
                     /locus_tag="SCJ86_000254"
     CDS             complement(19865..20344)
                     /locus_tag="SCJ86_000254"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344469.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonine/serine exporter family protein"
                     /protein_id="HEG1167848.1"
                     /translation="MGTIFIQFSFSFLATAAFAVITNVPRRSLICCGLSGAFGWMIYW
                     ICVQIGGSAAFGSLLGSLGVAFISDLFSKRLKMPVTIFNIPGMVPLVPGGLAYQAVRN
                     LVTESYREAAHYAVQAIMIAGAIALGLVLSEVFNHNIRNFKVKRDAIMMKRKKRLFK"
     gene            complement(20360..21112)
                     /locus_tag="SCJ86_000255"
     CDS             complement(20360..21112)
                     /locus_tag="SCJ86_000255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289867.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonine/serine exporter family protein"
                     /protein_id="HEG1167849.1"
                     /translation="MTGKELDLLLDTCLLAGKIMMESNAEMYRVEDTMSRIALASGNF
                     RLVSYVTQTGLFIGLDRTSTIRMEQITNRSINLEKVVKVNDLSRKYVASELTLEELYC
                     ELQAIDRDRSFFPVWLQIVSAAVISGTIMVLFGGKMTDLPVTLVIGGLGYAAYLYSLK
                     FFRIKFLSEFLSSLLIGIAAIYSVRFGLGSNQDLIIIGCVMPLVPGVQITNAIRDLLA
                     GHYVSGVSRGTEAMMTATMIGFAIAFIFQLFY"
     gene            21229..21299
                     /locus_tag="SCJ86_000256"
     tRNA            21229..21299
                     /locus_tag="SCJ86_000256"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:21261..21263,aa:Gly,seq:ccc)
     gene            21660..22385
                     /locus_tag="SCJ86_000257"
     CDS             21660..22385
                     /locus_tag="SCJ86_000257"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA/RNA non-specific endonuclease"
                     /protein_id="HEG1167850.1"
                     /translation="MSKKTTKAIVGIVAAVIAGAFGVSTTQKDSDIFQQITTIFDSTP
                     QASQVADVPEYDGKHQEIEINGNQPEFTKEDLSLSKGTWESYSNIDKLNRVGQANAML
                     GKDLFPTEKREPLYIDPTGWKQKKLSDGQWLYNRSHLIGFQLTGQNNNIKNLMTGTRS
                     LNAPYMLAHENDIAAYIKETNHHVRYRVTPHFEGNELVARGVQLEAESIEDKKIEFNV
                     FIYNVQDGYTINYETGQATKTKN"
     gene            22640..22906
                     /locus_tag="SCJ86_000258"
     CDS             22640..22906
                     /locus_tag="SCJ86_000258"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290885.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IreB family regulatory phosphoprotein"
                     /protein_id="HEG1167851.1"
                     /translation="MGFTDETVRFGFDDSRKKEVSETLAIVYRALEEKGYNPINQIVG
                     YLLSGDPAYIPRYRDARNLIRRHERDEIMEVIVKDYLSNHGVNL"
     gene            22903..23334
                     /gene="ruvX"
                     /locus_tag="SCJ86_000259"
     CDS             22903..23334
                     /gene="ruvX"
                     /locus_tag="SCJ86_000259"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345289.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction resolvase RuvX"
                     /protein_id="HEG1167852.1"
                     /translation="MRVMGLDVGSKTVGVAISDPFGWTAQGVEIIRINEAEEEFGFDR
                     LKELVAQYEVDRFVVGLPKNMNNSIGPRAEASMSYGEKIKELFSLPVDYQDERLTTVQ
                     AERMLVEQANTSRAKRKKVIDKLAAVMILQNYLDGSGKQLF"
     gene            23359..23664
                     /locus_tag="SCJ86_000260"
     CDS             23359..23664
                     /locus_tag="SCJ86_000260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002419177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1292 domain-containing protein"
                     /protein_id="HEG1167853.1"
                     /translation="MTEHNHSHDHEGHEHITLVDDQGNETLYEILLTVDGQEEFGRNY
                     VLLYPAGVSEDEDVELQAYAYIENEDGTEGELEQIETDAEWDMIEEVFNTFMAEEEE"
     gene            complement(23745..24890)
                     /locus_tag="SCJ86_000261"
     CDS             complement(23745..24890)
                     /locus_tag="SCJ86_000261"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306133.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HEG1167854.1"
                     /translation="MATFKQYETKKGKFWLYEAYLGMNKMTGKPDKVRRRGYKTKKEA
                     QLALSRLQVDYDKNGIKKANNETFKEVYDLWLETYKTTVREATFMKTEIKFRKWILPK
                     YGNLRVNEVTVKKAQQIVNSWAETTDQYKVLHSTSKRIFSYAINLGIIETNPLDNVLM
                     PKRTTTKHKEKVKVYSKEQLITLFDYLNSKPETYRNDFDKTLLRFLFYSGVRISEALA
                     LNWSDIDFQENTVTINKTLSQSKNGYKISDPKTDCSAGVLPLDERTISILKKWQINQR
                     KYMLTLGVTDPTMIFCGIYKELITHHAIYARLITICEKAMVPFLGVHVARHTHASMLL
                     DAGATMVEVQTRLRHTKISQTIDCYGHLAKETKEKTVEKLVQHLNID"
     gene            complement(24951..25598)
                     /locus_tag="SCJ86_000262"
     CDS             complement(24951..25598)
                     /locus_tag="SCJ86_000262"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296501.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HEG1167855.1"
                     /translation="MNGGENVARKKYPEAVETGQRIRLARIEKGLSMEQLGGKLSPPA
                     SKGAVSNWENGYNLPNNTRLKQLSKVLDVSTTYLLNGSYTLRDMHMMPEIKRNVLEKQ
                     LKKTIEKNNSIIDSNLSNAINNIEEDNLTVSQKIYLTNAIKYLKTGSRIEFPDGSTPI
                     AYLAALLNTLLRYDSLINDSEKNTDEKKEYQEFYENDLLKEFTALVTELNKHYSK"
     gene            25792..26085
                     /locus_tag="SCJ86_000263"
     CDS             25792..26085
                     /locus_tag="SCJ86_000263"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296500.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEG1167856.1"
                     /translation="MELKIDKLTLDDPAFKIIDDRVVQQVEQTQGTLLSLLEKKYQFP
                     KYMNKAQAAKYMNVSYNTMMQKYVPNGLKLIIVDGVVRISQDECDRFMEEYQK"
     gene            26129..26440
                     /locus_tag="SCJ86_000264"
     CDS             26129..26440
                     /locus_tag="SCJ86_000264"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296499.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEG1167857.1"
                     /translation="MESTQNKAIEKVLSTILTEDTAAELANLEGKALEETFECLYEQM
                     DYQKLLPQEPTVSGVLRGLNDLVQAEFKERLSIEEYQEILYQQVELLASLLGLELEDV
                     E"
     gene            26437..27061
                     /locus_tag="SCJ86_000265"
                     /pseudo
     CDS             26437..27061
                     /locus_tag="SCJ86_000265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350005.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="phage repressor protein"
     gene            27202..27360
                     /locus_tag="SCJ86_000266"
     CDS             27202..27360
                     /locus_tag="SCJ86_000266"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296496.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEG1167858.1"
                     /translation="MKRNLIIGSILIGGIFGLLPLIAKIAIFAGLALYLFTAYDEYDY
                     QLRTGGHK"
     gene            27361..27723
                     /locus_tag="SCJ86_000267"
     CDS             27361..27723
                     /locus_tag="SCJ86_000267"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306139.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEG1167859.1"
                     /translation="MYKTKLLNQLDSLELEEINQGIAELENNIGKTYFGNSFNEKLTV
                     LYVLKKHAEHKIICREINELKNQILTAWLNITDMQEARVKTFNTWVKYQNQLKGAEFV
                     RDGLKYELEQLKLMEVSE"
     gene            27760..28608
                     /locus_tag="SCJ86_000268"
     CDS             27760..28608
                     /locus_tag="SCJ86_000268"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296494.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional DNA primase/polymerase"
                     /protein_id="HEG1167860.1"
                     /translation="MANNYQAATELQKSGWSIYPLSPGTKIPIKGSSGWNDATNDSNQ
                     INEWWTEDATRNIGLMLEDHQMVVVDLDQHSETRNGVENYKKLASMYDPFPSTYTEIT
                     PGKGIHFFFKIPDGVIIQQETGAFADIFGRDDKGKSLSGIDIITKGVPIAPTMIISRS
                     VGRAYEPIENVSLNNIAPAPDWLVNLLLKKPQEIKTQYRPVAKTGTAKKLDMIVQGAG
                     EGSRNDHLTKLCGWLLWHGVDNQTLAELIYTANAYNEPPLKDKEVNQIIRSMIKKDLR
                     GKAHGS"
     gene            28598..30073
                     /locus_tag="SCJ86_000269"
     CDS             28598..30073
                     /locus_tag="SCJ86_000269"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296493.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helicase"
                     /protein_id="HEG1167861.1"
                     /translation="MAHKHKLDSILDFPEASEREDNIIELKTWMSRLRCNKDDQIKSN
                     SVVNAELILTNDSNLAGTIAYNEFSGYIHLLKDSPWINRTAGEWEDSFEDALTAYIEE
                     NYNVVFDDNKIHKAVVNVARKNVFNPVKERIEKVKWDQQPRLETLFIDLLGVDDNLYT
                     REVTKRWIVGSVARIYKPGIKFEIVPVLDGPQGIGKSTVPALLYTDDFFTDSLDSLGE
                     KKDDYMQLQGNVIIELGELSSMNKTKIEQIKNFISAKIDKIRPPYGRNVIAWARQCVF
                     IGTSNDGQYLKDDTGNRRFYPLPCKNKPKMNPFKTKDEYFLQVLAEAKVLFDKGQRIY
                     FSPDEDKEVLEIAKDYQEDAKMENPIKEAITKYLEMEIPYKWEEAPAWARRSYYQNYP
                     DSKCNEKNLQHFYNGATMQQEFYLVDSVLTADILEAVFDKQAKDLLNGRSDAETKKIA
                     LIISSIPGWERKQLPKRNKRRGFYNKSNAYENKNRAGKYKK"
     gene            30339..30746
                     /locus_tag="SCJ86_000270"
     CDS             30339..30746
                     /locus_tag="SCJ86_000270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3206 domain-containing protein"
                     /protein_id="HEG1167862.1"
                     /translation="MLNIISTNKAPNFQYTDEMDRFLMNTLAFSVGLVTEDYSTFDPE
                     VLKIMEEEPDWLQESVAWCQSLVVGSLVDSGNYDDTGELMDEFNCLLNLYDRARQREL
                     TSNEDNLFLNIHDKFLALLLTDDELITNLLEVE"
     gene            30749..30964
                     /locus_tag="SCJ86_000271"
     CDS             30749..30964
                     /locus_tag="SCJ86_000271"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296491.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEG1167863.1"
                     /translation="MHMESIRYFDGEKHIEYSNTQEDVDFISFEEDKITKVNFKDGTC
                     LKIVSPYIEYKSKWEQDDSDERDRFGW"
     gene            30968..31348
                     /locus_tag="SCJ86_000272"
     CDS             30968..31348
                     /locus_tag="SCJ86_000272"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304527.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HNH endonuclease"
                     /protein_id="HEG1167864.1"
                     /translation="MAVKKQCNHAGCKTLIDYRQKYCGKHKAKQTAIKREERKQSEGK
                     YFQFYQSRTWRKASYLYRLNHPVCEDCLEEGLIRKADVVDHKIELKDDWNRRLDETNF
                     RSLCHAHHNSKTANERQRREKSTL"
     gene            31482..31637
                     /locus_tag="SCJ86_000273"
     CDS             31482..31637
                     /locus_tag="SCJ86_000273"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010780558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2292 domain-containing protein"
                     /protein_id="HEG1167865.1"
                     /translation="MKVGDLLKNTIVITDKTGKQRTIELPHFGQVTIQMQNGKIIYID
                     KLDKEKF"
     gene            31706..32179
                     /locus_tag="SCJ86_000274"
     CDS             31706..32179
                     /locus_tag="SCJ86_000274"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296488.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage terminase small subunit P27 family"
                     /protein_id="HEG1167866.1"
                     /translation="MGHPKILHDTKGNISSEEKAIRVDAREELFKQQPLINITPPDWM
                     AASARSEWNRIVPTLKKDYPLSEADYGSLVAYCLAFARMKTAEAEIRKSGTFITCENG
                     VKKANPAVRVQSQAMSDLKKQATSLGMTLESRSKLALNKVKNDEPEDPFKELMNS"
     gene            32176..33870
                     /locus_tag="SCJ86_000275"
     CDS             32176..33870
                     /locus_tag="SCJ86_000275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336295.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="terminase large subunit"
                     /protein_id="HEG1167867.1"
                     /translation="MNDYIEKVLSGKLIAPKKIIQACERHISDLERSKSDSFPYVFDE
                     EQATKAIKFIELLPSTDGKAIKMLGFQKFILGSLYGWRTKEGNYRRFNRAFTSMSRKN
                     GKTYIASGMAANALIMEQEPAEARQVLFVSNALKQAKLGYDMLSNSLRNVVKSSKFLR
                     PQLKIMNSKIQHLPSNSFAMALASETSTLDGFAPTTAILDEWHEAKTRKTYNVIKSGM
                     TQQKNGLLCVISTAGLDLNVPMYEEYLLLDRVLKGEEQADRYFIAIWELDDPEEIHDQ
                     EKWIKANPIFESEEIKKVMIPTIQDDVNLALKQNNLNSVLVKNFNLWRQASEDSYMIA
                     EDWQATEVEPQDITGKPVYIGVDLSKTDDLTSVSWIVPLDNGELYCDSHSFVATKYGL
                     QDKEKRDGLPYRELEKAGECSITQLESGIVDYDQVFQFIQDLIQENDLECMGICYDPY
                     NANSLISKAEKANYPMLEVRQGTITLNVPTRTFREQVYEGNVIHNKNTILTHAVNNAI
                     LKTDNNGIQINKSKNSNKIDPIAALINAYVFAMDYFTTTEGAKADNEFYTSEEFSF"
     gene            33836..34021
                     /locus_tag="SCJ86_000276"
     CDS             33836..34021
                     /locus_tag="SCJ86_000276"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEG1167868.1"
                     /translation="MNFIQVKNFLSNYIHTVLLLLGLVCVLVAITLLTNVYYGLLALG
                     IVLIGVAVMLNTEQKGG"
     gene            34025..35200
                     /locus_tag="SCJ86_000277"
     CDS             34025..35200
                     /locus_tag="SCJ86_000277"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005879175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage portal protein"
                     /protein_id="HEG1167869.1"
                     /translation="MAFFKARQNTTGDPFLQHVVSIQSDDYTTSFTSVRALRNSDVFA
                     AVRIIASDIASSPIQLVKNNMPQADNELVKLLNEKPNSEMDGWHLKFALAVNMLLNGN
                     SFAEIKRNGEKVEEIHLLPNSSVTVTQLDNGTLSYQIGDKKRRVKSSDILHFKYFTQD
                     GLTGLPPLYALRDELKIQQAGNRTLHNFFTRGVSGSGILKVHKSDLDGSAKNAIREKF
                     EEANGSSSGDNALRTIILDETMDYKTLEINTDVLKLINSNDWNTKQIAKAFGVPIERL
                     GVENEHSSTVQSNLQYIQSTLIHYFNVFVSELDTKLETNIRFNSDQLLETDPETKVKN
                     VLDQVKGSLLTINEGRSKMGLPPMDGGDRLLASLNFTYLDTLEKYQLKEQEGVTPVE"
     gene            35193..36716
                     /locus_tag="SCJ86_000278"
     CDS             35193..36716
                     /locus_tag="SCJ86_000278"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296485.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage major capsid protein"
                     /protein_id="HEG1167870.1"
                     /translation="MNNEEEKEKRLTEEAELTADSPKVEKENEKQPTDGKTISGYALK
                     FGQPSKDLGGFVEVITPEALKEVDLSNVFLLYGHDYSKPLASVKAGTLKLNVDDTGLH
                     FEAELTDTTYSNDVYENISKGVVDSMSFGFVLGLDSFDKKEDGTIERSIDKIKALNEI
                     SVVTVPAYDSSNVQVNKRSYESFMSNNQAKQTNNSLESTSKAQKESNNMEKTLIDNEK
                     TELRGYEEYIRSQGEVRDGVTTVNAAAVVPEEVIGEVFDLKRSNYNLAQYATVKTVSN
                     GQGKYPVATNQQAVLATKAELAEIGDIDAEMFTSVDYKVETRAGKIALSNEVVEDSAV
                     NIVQEVKDQLAKLVENTDNKHIMDLLKTFTKKTAATLDDLKQLYNVALDPALNKMVIL
                     NQSGYYHLDTLKDSDGRYILQPDVTAPSGKSLFGMPVVLIADTLFANPKAGTFPMIMG
                     DIAQSIFVARRNQVTTQWEKFDYYSQGLAVIVRNDYKKIDQNASVYIEFTPVVTPKV"
     gene            36772..37056
                     /locus_tag="SCJ86_000279"
     CDS             36772..37056
                     /locus_tag="SCJ86_000279"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296484.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage gp6-like head-tail connector protein"
                     /protein_id="HEG1167871.1"
                     /translation="MVILDSIKKSMRIDHTIDDDFIQQLIDTAGEYIKSAIDSSATDK
                     DMDNYQQFDLAVSLLTQHWYLNRQEASSERIPVTVQALVQQMRGAYYANH"
     gene            37043..37378
                     /locus_tag="SCJ86_000280"
     CDS             37043..37378
                     /locus_tag="SCJ86_000280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336299.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage head closure protein"
                     /protein_id="HEG1167872.1"
                     /translation="MPIIKNVNELTERVKFKKTKRVKDEDGQMVDSEETVFECWANVR
                     SQMLKDVLASVGTILEGTLTFIIRYDQDYELTNDMKVAWKNKSYKIISINEGTAFKDY
                     TTIIAKQIS"
     gene            37673..38299
                     /locus_tag="SCJ86_000281"
     CDS             37673..38299
                     /locus_tag="SCJ86_000281"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296481.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEG1167873.1"
                     /translation="MDKAEFTNFYWKYYLHLENEFINTTDFVMLDENNFKTFSIEYQK
                     LLLAIGSECEIIFKELCGFDSSSNKSITDFKAVIKSSELMCLDNGVRVMNSLTLTSLK
                     PFGDGWPEDTPDWWKIYNRVKHGRSSNYKEANLENVLYALASLYLLEEYLHKKVILEG
                     EVDFISPESNLFTLSWERKHSSMQKVTFEKVDSNYVAPLIDFKEQEEI"
     gene            39041..39878
                     /locus_tag="SCJ86_000282"
                     /pseudo
     CDS             39041..39878
                     /locus_tag="SCJ86_000282"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325086.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="sulfite exporter TauE/SafE family protein"
     gene            39943..40377
                     /locus_tag="SCJ86_000283"
     CDS             39943..40377
                     /locus_tag="SCJ86_000283"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853061.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEG1167874.1"
                     /translation="MLREMRLEDAEALREINADQLGYDIPLTVTVRQMKKLLKEPEKN
                     FFLVYEDPTSKKAAGYVQAESYESIFSDPMFNIMALAVSKYAEKRGIGKALMTGVEEE
                     AKKRGITTIRLNSAEYRVEAHQFYEHIGYHSDKMQKRFLKIL"
     gene            complement(40445..41767)
                     /locus_tag="SCJ86_000284"
     CDS             complement(40445..41767)
                     /locus_tag="SCJ86_000284"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302984.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-hydroxycarboxylate transporter family protein"
                     /protein_id="HEG1167875.1"
                     /translation="MAQKTTTATAVEEKKKEGFWSTKITGVSLPLYLIMVLVLIIAMA
                     LGKLPTNMIGPIAVLVIFGNLFHFIGTKIPIVKSYLGGGSVFAIFASAAIATFGILPE
                     YVVKSTENFVSNMGFWDFYIAALITGSILGMNRNLLMKASVRFIPVALISMVVSFFAV
                     GLVGMLIGNGFANSVLYISLPAMAGGVGAGAVPLSTIYANVLGTDASSIISRLIPATA
                     MTNVLAIIGAALVARAGQSMPKFNGNGKLMEKGDLGDGKPRTVKPSIPQLGVGLMVSL
                     TFFILGQIGNYFVPKVHAYAFMIIIVVICKIANLLPEYYEDAAIMFNNLIVKNLTAAV
                     LAGIGIALLNLNVLASALTWQFVVLCLTSVIAISVVSGFVGRLFGLYPIESTITAGLC
                     NNSMGGTGNVAVLSAANRMELIAFAQMGNRLGGAIVLIISGFLMQLLS"
     gene            complement(41921..43561)
                     /locus_tag="SCJ86_000285"
     CDS             complement(41921..43561)
                     /locus_tag="SCJ86_000285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345286.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent malic enzyme"
                     /protein_id="HEG1167876.1"
                     /translation="MLTGLNLLNNPFLNKGTAFTKEERAKYGITGMLPSTVQTLEQQS
                     VQAYGQYLSKQTDLEKRIFLMNLFNTNRTLFYKLMGEHLVEFMPVVYDPVVADSIEQY
                     NEIFLKPQDAAFLSIDEPENIKASLKNAADGRDIRLIVVTDAEGILGMGDWGVNGVDI
                     AIGKLMVYTAAAGINPAQVLPVSIDAGTNNEELLNNPLYLGNRHARVEGETYYEFIDQ
                     FVQSATELFPELLLHWEDFGRGNAAAILEKYEDQITTFNDDIQGTGIVVLAGVLGGLN
                     ISGEQLKDQIILTFGAGTAGVGIANILLDEMIRQGVPEEEARQHFYQVDKQGLLFEDT
                     EGLTPGQIPFARKRSDFTNADELTNLEAVVKAIHPTIMIGTSTQPGAFTEEIVKEMAA
                     HTPRPIIMPLSNPTKLAEAKAKDLIEWTDGKALVGTGIPAADVEYNGVTYQIGQANNA
                     LMYPGLGLGLIASTATRVNAEIISQASRALGGIVDVTKPGAAILPPVAKITEFSQTIA
                     ETVAKSVVAQNLNREEITDIKEAVESAKWVPEYKSLED"
     gene            43891..44361
                     /locus_tag="SCJ86_000286"
     CDS             43891..44361
                     /locus_tag="SCJ86_000286"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295059.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4809 family protein"
                     /protein_id="HEG1167877.1"
                     /translation="MKEVQLIRKSELSEGGCNACGVVEATSYTLKLDSNQAIISELTV
                     GGLVDSLALAEGFTGEDIYEMFSEVRQLKKGEKCIEVHHESPNVRFKRGDNEMIFKNH
                     VSDHTELYEIVNQILSGIFELGPYEFKEENGNPKLNEEWQETIETQRNNPHLFQ"
     gene            44990..46093
                     /locus_tag="SCJ86_000287"
     CDS             44990..46093
                     /locus_tag="SCJ86_000287"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-hydroxy-acid oxidizing protein"
                     /protein_id="HEG1167878.1"
                     /translation="MEKTYQAGTNEGIVDFINMEDLEIAASQVIPAGGYGYISSGAGD
                     LFTYQENERAFNHRLIIPHVLRDVELPDTTTHFDEEMLTAPIIMAPVAAHGLAHVKAE
                     KASAKGVADFGTIYTASSYASCTLEEIREAGGEKAPQWFQFYMSKDNGINLDILEVAK
                     RNGAKAIVLTADATVGGNRETDRRNGFTFPLPMPIVQAYQSGVGQTMDAVYKSSKQKL
                     SPKDVEFIAAHSDLPVYVKGVQSEEDVYRSLESGAGGIWVSNHGGRQLDGGPAAFDSL
                     QYVAEAVDKRVPIVFDSGVRRGQHVFKAIASGADLVAIGRPVIYGLSLGGSTGVHQVF
                     DFFKTELEMVMQLAGTQTVEDIKKIKLRENRFI"
     gene            46377..47057
                     /locus_tag="SCJ86_000288"
     CDS             46377..47057
                     /locus_tag="SCJ86_000288"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629126.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="WxL domain-containing protein"
                     /protein_id="HEG1167879.1"
                     /translation="MKKNMIGTLLLSTLLLGGLATPAFAEGQATSKGDITFTEPTNTV
                     EPLNPTDPSKPVEPADPENPATGQTGSLTLDVVPELPFGTHEIESGTKTYQVDASKND
                     TPYLQVSDRRGVGADGQAQGWNVTVSVSDFVNGSQVLQGAELDFGTSTVKSTSDNEST
                     GPTSQTVTGLSKASAATPIFTAAKDQGLGTWLSVYDPANITLKVPKAAAGTFTADLTW
                     NLVAGPVA"
     gene            47147..48181
                     /locus_tag="SCJ86_000289"
     CDS             47147..48181
                     /locus_tag="SCJ86_000289"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296471.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF916 and DUF3324 domain-containing protein"
                     /protein_id="HEG1167880.1"
                     /translation="MNQSKRLFVSFFSILSLFFIFPSISQAKDSAGDFGIKPVFPENQ
                     IDKAIGYFDLLVAPEQNQTLEVIISNSSDEERTFEVSVNPAVTSDGGTIDYSQKNPTL
                     DETLPFDVRDVLLIAKKEINVSAHAETTIPIEVKIPAKSFKGRVLAGIHVSPKEEAET
                     ENATEGAQIKNRIAYNLAVVLQESQETIEPDLKLLSGDLDEVNAKPTVQLRFQNPQPR
                     IISNLIFTSKIFYENQLYIENTSNEFLVAPNSNFHLNLDLAGDKAKAGDYRAEIIAKS
                     GDSNEWRFTQNFTIKKEKAQKVNENSVFAVQEQSFPWLYAGLVAAVLVLWLILLFLWV
                     HKKKKKGETT"
     gene            48195..48530
                     /locus_tag="SCJ86_000290"
     CDS             48195..48530
                     /locus_tag="SCJ86_000290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325081.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEG1167881.1"
                     /translation="MKTSILLGGIIASLLFFSWQEPVQADTKSSGTITFYDLSPVPHP
                     SGSVNGASKQQTQLPIYKESNQHGVFPETNEEKIRFLYLIIGIELWLLAWLLLWRRKE
                     KEDEHVATK"
     gene            48514..50994
                     /locus_tag="SCJ86_000291"
     CDS             48514..50994
                     /locus_tag="SCJ86_000291"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326300.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEG1167882.1"
                     /translation="MLLQNKFSFFRSFFFTSLFVLSIFIFSPSAEAVGATPPAISYDP
                     YTYENIVPPNGGRSAVNGGKLVQLTGANQLPKLTATYLNDGSNDPWKGGTVRFHWESH
                     VKLTNVLDLVNITAQGFAFVAIINLPEHVSSSDVLKAIDWNNAYLYIAGAPIKVTSGS
                     LLSAGSHTLRFGMGSWDTNTLLADIIAIITRGEFNVDDIPLQFDVTANVAKMTENGDK
                     YDTSPNKILTKGKLQPSMSKKAVFSVDFYDSNNIIQDRMFNSILAPAAGRFYPKLTGG
                     NLNPGAYAFSASAEINTWNSYISTSDSTGKYNTFADTTEYVDGNNQNLPGSTNSRTLT
                     MGLTEGRFDQLPFSRFDRVVNYFTGKNVTQGAILTHTPASTSGLNQTANIVYSGKDAQ
                     GGLLSPVALKVLEQYKLDGTVLPTNLTADSQWGKLLSSASYQVTANWSATELKKGSIH
                     YRLYSKGTQKLATGWTDQLFQTIQSNNGGVNTATSTLPALASGQYYFDYRLVDDVLTN
                     MYPSFAYKWQSEQAGITTLPQITVANFPSVSADSQLKNLSRQPETGDPLSALSGDTIQ
                     ENVTFTLTKSGDAITDKKITISLPENTTYINGSLKLNGTTIADTGIQQGVSVPADLLS
                     KIGDTIHLTYNYQLNTVDTSVQSVSILTKAAVLSSNITLADGAKLPNPVVQTSAKTIL
                     VPKQELTLVNVPDDFTFGNDLPKPLKTSYYEAKGDFSFDVRDTRLPSTSPWQLTGTLT
                     SLFKNDQGQELSGTKLYFNHSGSKQLIQQGQNTLIYESDGTAKGEVLVDFPDTDGLLL
                     EVNSSTNAQPGATYQGMVTWELTAGPTS"
     gene            51050..52609
                     /locus_tag="SCJ86_000292"
     CDS             51050..52609
                     /locus_tag="SCJ86_000292"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306164.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HEG1167883.1"
                     /translation="MRRKLLLLKSEMLGGVDLELFSLLEKNEQRQIKLFQLLLEEKGE
                     ISVNNVLTRLSVDRATLKEDLFYLQANLENIKQLEIVIQEGNILLIRHGNIAAVDVYY
                     HCYLRYSTKYQILLYLLENGSFDRQKLVMELNISSATLSRRVKELNDSLNEFHLKIKS
                     GRLTGPESQICYFYFQLFWFGRPYLENKKEYSDLSGNTFLDLLQKEFSFTITEEGRLK
                     FEIWLSIMKKRMRCTKKSKPDYSNLPVGLFQTTQFLLDLHTFLTRYFFQYAFPWNQNE
                     TLVFYLFMISNFTLDTHNSHVQEFLISEKYKDDVVAQLNRTFKQSIEEYFSFYVFPEE
                     ISQKIALTVMQNHAHFAYFRGWMNIFGQEGLKQRLTEVEDKQFLVLCERLVRQTFAIV
                     DFTEVDEENARFELFARYASILRTLIKQLELRLQIAYDLPYERLMADIVKEIIQEQLL
                     NTIKYDLVEYEKEKQYDIILTSQLKEYPSQKSAKVFVFTSNEYNYDFPYLNQFLKECY
                     LEKLNLRMNKT"
     gene            52606..53970
                     /locus_tag="SCJ86_000293"
     CDS             52606..53970
                     /locus_tag="SCJ86_000293"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HEG1167884.1"
                     /translation="MKIVIIGASHAGITAALNLRKLQPKAEVLLIDEDHKDGLGYVSN
                     GINLYLKGKIRSLAEVAHNMRTLQESGAKLITEWRVTELDPDKHQLILASKEGKNETI
                     TYDKLIVATGSSPVTLYKQIEAENVYTYKNLVQSKQVLAALKEAKEVVIFGAGYIGLE
                     LADALRNKGYIIHLVDYMPNVLSRYFDKDMINSFQNQLQTKQINFYPNEFLIDWKKSE
                     EKVVSVQLLSQAIKADMVIFSAQTRPNTTLLKEKVALYEDETVIVNEYLQTSDPDIYA
                     IGDIVPVSFDKNKRHLFLPLVTRAVHMARAVALTLSGQPTAYDLRQKITAAVIIDYFL
                     GTVGLTEDEAPFLEQNTGSCSGEFDLFPQYDEENKTVNAKLIYHPDTLEIIGGQLISQ
                     EFLLSDLNLLADIVKHKTTIPQLAVEDFGFLSEHTSRFHYLNELAFKVLAKRANSNRV
                     DKRL"
     gene            54141..55211
                     /locus_tag="SCJ86_000294"
     CDS             54141..55211
                     /locus_tag="SCJ86_000294"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631334.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEG1167885.1"
                     /translation="MFLALNEIMHSKLRYALVAGVMFLIAYLVFFLTGLAYGLAQDNR
                     TAVDKWEADSIVLSKDANSNLGMSMITKKIAEEVEGGKVAYLAQTPGVVTSKDSTEEG
                     KINVSFFGIDKNQFIMPNLVEGKAFDNDDEAVGDISLKEEYGLAVGDTVKLSGSDKTF
                     KLTGFTDHAKFNVSPVLYTTINAYQEIRFEKEDTSENARINAIVVRGKISNLPEDLEQ
                     IKISKFINELPGYNAQVLTFGFMIGFLIVIAAIVIGIFIYVLTMQKINIFGVMKAQGI
                     TGGFIARSVVAQTFILSFVGILLGLLGTVGTSLVLPDAVPFQSNWLFFGVISLLMLVV
                     AVLGALFSVRTIVKIDPLKAIG"
     gene            55226..55894
                     /locus_tag="SCJ86_000295"
     CDS             55226..55894
                     /locus_tag="SCJ86_000295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375790.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEG1167886.1"
                     /translation="MKAIEFKEVEKSFQDGDQVVKALKETTMSIDKGEFVAIIGPSGS
                     GKSTFLTLAGGLQTPSSGSVFINDQEFSNQKESERVKLRFKEIGFILQASNLVPFLTV
                     KKQLLLVDKIKHEHRQKEAKELFEQLGVDKLINKYPEELSGGERQRVAIARALYNDPS
                     IILADEPTASLDSEKAREVVDILAKESKDKNKATIMVTHDTRLIDKCDRIFMMEDGVL
                     KEKE"
     gene            55914..56492
                     /locus_tag="SCJ86_000296"
     CDS             55914..56492
                     /locus_tag="SCJ86_000296"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HEG1167887.1"
                     /translation="MVHRYNKDTQQKILKEATDLFMSKGYLGTSTREIAQKVGITQPN
                     LYHYFGDKEKLYTAVLENHLKDVGKALREIVQTSETGFQVTLTKMAQFLIETHLVDLF
                     LMLHDLESNLSKETRDHLFFLWKKNYREPFEEIFSANQSVLRNGISKEIAARHFFLVL
                     APYITRSKRPVEEPLNVAQLIDLYLNGVLGEA"
     gene            56895..57806
                     /locus_tag="SCJ86_000297"
     CDS             56895..57806
                     /locus_tag="SCJ86_000297"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337333.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase"
                     /protein_id="HEG1167888.1"
                     /translation="MKKIIGAFVFAIIIIIICFQIDVFGQKKIVGQTVSSTNSTVTKT
                     KESFDDTEQETLIKIGKNDRTLYTDDSLTTPLATINGGELAEFLSETKNAYQIKTNDG
                     YTGYLALEDGTKVTKNIQTKPEELSDAVIVLDPGHGGNDTGALSNDEQTEEKDITLST
                     AIKVKKALEDAGATVYLTHTTDELVQLGDICDYSEEKKADVFISLHADSTEYANEATG
                     ITTYYYYGQEETLAQTIADSFTDLPLDSRGISTGNYQVLRENLQPSILIEMGYMNNDS
                     DLKELVTDSYQQQIAASLTQALTTYFQ"
     gene            complement(58062..58979)
                     /locus_tag="SCJ86_000298"
     CDS             complement(58062..58979)
                     /locus_tag="SCJ86_000298"
                     /EC_number="3.1.26.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295085.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease HIII"
                     /protein_id="HEG1167889.1"
                     /translation="MSNVIIKATNDQLSQMKAYYRSYLLNKQIPYTSFAAKKDGTTIT
                     AYTSGKVMFQGTNAAIEADKWGEAQDKQAKPAKTASSLPKNISQLSVLGSDEVGNGSY
                     FGPLTVCAAYVKRDQLAELRKLGVRDSKELKDSQIIQLADILKKTIPYKLLVLSPEKY
                     NEIQPKYNAVRMKVALHNQAIHLLLKKIEPEKPEAILIDQFTPEANFKKYARLEKNQL
                     QEKLYFVTKGEQYHLAVAAASIISRASFLEELHKASAEAGITLPSGAGTKSDQVAAKL
                     LEKGGMPMLEKYAKLHFANTEKALKLLKK"
     gene            59181..59618
                     /gene="zapA"
                     /locus_tag="SCJ86_000299"
     CDS             59181..59618
                     /gene="zapA"
                     /locus_tag="SCJ86_000299"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340190.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein ZapA"
                     /protein_id="HEG1167890.1"
                     /translation="MAHEKTRYKAVIANQTYTIIGRETKHHMDIVTKLINEQLAELKQ
                     LSPQMDNEQAAILMAVNALSDQLKKQERILELEEETAELKKKMIKFTELENRVKRIEA
                     IENEAREVLKENGQADHTIKNHVEAQQILNEKRKGQIKQKSSN"
     gene            59676..60223
                     /locus_tag="SCJ86_000300"
                     /pseudo
     CDS             59676..60223
                     /locus_tag="SCJ86_000300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629130.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="CvpA family protein"
     gene            60338..62698
                     /locus_tag="SCJ86_000301"
     CDS             60338..62698
                     /locus_tag="SCJ86_000301"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease MutS2"
                     /protein_id="HEG1167891.1"
                     /translation="MNNRILETLEFEKVKQMVRQFVVTAQGKEELAELVPVSEKQTIT
                     NWLQETEDGLKVQRLRGGIPIPKLENIRPHMKRIEIGADLNGVELAQVSRVLSTTSEL
                     NRFIDDLSDSEIEFARLYMWADQLVTIPVLSRRLKEAIDEDGRVTDDASPELKSIRQN
                     IRRSEQAVREQLDGIVRGKNAKYLSDAIITMRNDRYVIPVKQEYRGVFGGVVHDQSAS
                     GQTLFIEPKQVVDLNNRLRQYQIAERNEIQRILSELSAELVPHRQEIIHNAYVIGKMD
                     LMNAKARFGKEVKAIVPGISEDNHVVLKQARHPLIDQEKVVSNDITIGKDYQAIVITG
                     PNTGGKTITLKTLGLLQLMGQAGLPIPAGEESQIGIFEEVFADIGDEQSIEQSLSTFS
                     SHMTNTVDILSKVNEKSLVLFDELGAGTDPQEGAALAIAILDDLGKKSAYVMATTHYP
                     ELKVYGYNRANTINASMEFDVDTLSPTYRLLIGVPGRSNAFEISSRLGLDTEVIDEAK
                     QLMNDESQDLNEMITDLENRRKMAETEYLEMRHFVSEAQELHDDLKEAYSYFFEEREK
                     EMEKAKKKANEVVSEAEEKAEKIIADIRKMQQQIGQGNVKEHQLIDAKTQLANLHQEE
                     TLKKNKVLKKAKEQKTLKPGDEVLVTTYGQRGTLLRKNGNQWQVEIGILKMNVSEDEL
                     TPVAPQKEPTQRVIHAVRSESSSHVPNQLDLRGKRYEEALSEVDQYLDSAILAGYPQV
                     TIVHGKGTGALRKGITDYLKNHRSVKSFEFAPANQGGNGATIVKFK"
     gene            62785..63099
                     /gene="trxA"
                     /locus_tag="SCJ86_000302"
     CDS             62785..63099
                     /gene="trxA"
                     /locus_tag="SCJ86_000302"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290803.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin"
                     /protein_id="HEG1167892.1"
                     /translation="MSQVITDKTFNEETDKGLVLIDFWATWCGPCRMQAPILDQLEQE
                     YDEEEFRIAKMDVDENPETPQQFGIMSIPTLMLKKDGQVVEKAVGVHSKEQLRQMIDQ
                     YL"
     gene            63281..65059
                     /gene="uvrC"
                     /locus_tag="SCJ86_000303"
     CDS             63281..65059
                     /gene="uvrC"
                     /locus_tag="SCJ86_000303"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346080.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrC"
                     /protein_id="HEG1167893.1"
                     /translation="MNERIKNKLALLPDQPGCYLMKDKNGTIIYVGKAKILKNRVRSY
                     FTGSHNTKTERLVSEIEDFEYIVTESNIEALLLEINLIKKNDPKYNIMLKDDKTYPFL
                     KITNEKYPRLVITRKVLKDKALYFGPYPDVGAANETKKILDRLFPLRKCKPSQTKEPC
                     LYYHLGQCLCPYYFDVDPKVYTKIVDEVKKFLNGGHEKIESEIHQKMEKAAENMEFEK
                     AAEYRDQIRAIETIMTRQKMTNTDLLDRDVFGYAIDKGWMCVQVFFVRQGKLIERDVS
                     MFPFYNEAEEDFLTYLGQFYQENEHFIPKEVLIPDTIDKPSVEALLATKVLQPKRGEK
                     KKLVELANKNASVALNERFDLIARKQERTIGAVERLGNAMNIPTPIRIESFDNSNIMG
                     TNPVAAMVVFIDGKPARKEYRKYKIKTVNGPDDYASMREVIYRRYSRVIREGLPLPDL
                     ILIDGGKGQVDVAKDVLENQLGIDIPIAGMAKNDKHKTNELLFGIDLAVVPLERNSPE
                     FFLLQRIQDEVHRFAITFHRQLRSKNSFASRLDEIERLGPKRKKMLLKEFKSLKNITA
                     ATTEELQEIGLPKNVAQNVFDKLHQE"
     gene            65204..66103
                     /locus_tag="SCJ86_000304"
     CDS             65204..66103
                     /locus_tag="SCJ86_000304"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313470.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEG1167894.1"
                     /translation="MLEVKDLVKTFGNYTAVDHVSFQIPDGKIMGLIGQNGAGKTTTF
                     RLILDFLTQDQGEVLWNGHHLGEKDYDVIGYLPEERGLYPKVTIQDQLLYFAELRGKS
                     RKEIEPKIDFWMEKFQVKGKKTDKVKSLSKGNQQKVQLIATLIHEPKLIILDEPFSGL
                     DPVNAELLKDGILELKKHGSCVIFSSHNMDNVEKICDHLIMLRNGKMVLNGKVHEIRE
                     SFGRTKLFLESGLSQQEVEEIEGVKKVILHEDNTLEITLEDPEAGKEIFTRATQFGYI
                     PMFNQQPPTLEEIFKLKAGEIDA"
     gene            66096..67358
                     /locus_tag="SCJ86_000305"
     CDS             66096..67358
                     /locus_tag="SCJ86_000305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333751.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEG1167895.1"
                     /translation="MRKFWIITKDVYLKNVKSISFLIMILVPFIMLGIIYVAGNFAQQ
                     NSDVNKIGVLADNEQISQQMSQVKTDDFEFKAVDSEEKAQKELSEEKIDAYLVVSTEN
                     DKINGMLYSENSLGQSTQLLIQQQLTGLQSMLRASQLGISPEEVASLSQQASLTRQKV
                     NFDSKGKMTVGEDNSTVQYVVSYVATIVLFIIILTYAQIIAQEIASEKGTRIMEVILS
                     STTAQKHFYGKLTGVLLVALTQMALYGIIFGIGYSQFKDMEMVKNILDGISLDNLIGP
                     FLWYSLLFMIFGILIFSVLAALCGSLVNKAEDTAKAILPVTYLSLGGYMLGLILGASD
                     PNNIVIRITSYIPFLSSYIMPVRLANETVGMGGALISLLVLVAVTFLLMILSANMYKS
                     NVLVYSEGGLWSSLKQSISIMRNERKKA"
     gene            67521..69419
                     /locus_tag="SCJ86_000306"
     CDS             67521..69419
                     /locus_tag="SCJ86_000306"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290807.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fructose-bisphosphatase class III"
                     /protein_id="HEG1167896.1"
                     /translation="MDRYLDLLSEKFPTTEDVITEIINLEAILQLPKGTELFLSDIHG
                     EFPAFDHILRIGSGNLKEKIKDLFSEQMTETEISQFTIFTAYPEYVLTTDWYKKQDKS
                     QLIHRLIDLLRFTTVKYTRSKVRKGLPKEYSYIIEELLYIDDRINGKKDYVKKIIEQL
                     VLMKEEEKFLTKLAQTIQKSVIDHLHIVGDIFDRGTQAAKVMDRIMDFSSVDIQWGNH
                     DILWIGAYCGSEACLLTLLRIAARYNYLFELEKEYGLNLRPLFSFAHQTYQKNPVFTP
                     KNRKDLSEREQEELEKIHQALSILQFKSEHQLLNRRPEFKMDDRKLLEKINYEESTIV
                     LEGQLYELKGTCFQTIQPDDPKKFLEEEQKVIDSLMYSFQHSLRMQKHMTFLIEKGGM
                     YLTNNQHVLFHGCIPVDEKGQLLAFELDQSYRGKELLGFFEKQITESAKDLTANEDLA
                     TDLIWYAWSGPFSPLFGKSKMATFERYFLTEPHTHVEKSNPYYHLRDEEWFSEKILAE
                     FDAKEEGSAIINGHTPVKVKKGESPIKANGKAFVIDGGLSKAYQKTTGIAGYSLLCNS
                     YGFQIVTHYPFLPIEQQFAKKSDQTNVKKVIEQQLPRKLNRNTTKGMELQQQITQLQR
                     LLDYRKDD"
     gene            complement(69718..70437)
                     /locus_tag="SCJ86_000307"
     CDS             complement(69718..70437)
                     /locus_tag="SCJ86_000307"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gamma-glutamyl-gamma-aminobutyrate hydrolase
                     family protein"
                     /protein_id="HEG1167897.1"
                     /translation="MKPIIGIAGNQLIRATDTFQGNQVSYTPQGFVDAVLDAQGLPLI
                     LPVMSPDSAPQLIEKIDKLILAGGQDVSPQLYMEDPHPKLTETNIQRDQFEQALILEA
                     LKQRKPIFAVCRGLQLLNVVLEGTLYQDLSLYPKWSVKHEQQPTAPQFATHEVKIVSD
                     SLLSDLLPDSYFVNSYHHQAVKDLSPLLKAIAFSNDGLIEAVQSKDNMHKILGVQWHP
                     ELSHRVDSSEQRLFDFFVNEF"
     gene            70774..71106
                     /locus_tag="SCJ86_000308"
     CDS             70774..71106
                     /locus_tag="SCJ86_000308"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEG1167898.1"
                     /translation="MKQVLEALEQIRKAEEENEKRARTLRQQLKTYAEEKEQELEIIQ
                     TNSRQQLKQLVEEKEQFEKQELEKEKMKLKAEADKVQATLKDQYQKHQSQAIGAIIER
                     VKETYGRH"
     gene            71093..73096
                     /locus_tag="SCJ86_000309"
     CDS             71093..73096
                     /locus_tag="SCJ86_000309"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346472.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATP synthase subunit I"
                     /protein_id="HEG1167899.1"
                     /translation="MAVTKMEKVTLISDKRNQERILQAVQGIQNVEIRDLYQNRTNNQ
                     WVEKYFPDTTIIDKEAKLSALGNRLKDIQEAIQFIQHHGDSRQKKLHLKRQELSLQDL
                     EANYSEENFMNKLEEVLDLKEKWEQLTEKRDRLEEEEEWLLHWQQLDIAPSMYASKTT
                     IFEMGTVSAANFESFKEELSKLDDVYMEEIDYTPKQVYLSYVALKKSVAAIEEIAGKY
                     GFNKETYPYEQNPKEKLQETKQQLSIVHEQQKKITTALGQCSGYIQEFEWVEEVTLAV
                     AEREKVKERFIHATYLIVLQGWVDAEEKQNLMHVLNETVSEEDIYVSFETPNSEEIQQ
                     EVPTKLKNHPLIEPFELLTEMYSLPKYEEVDPTPWMTPFYLVFFGMMVADVGYGLLML
                     IGTILAQKLFVLPRGMKRFVKFFEILSVPSIIWGLIYSSFFGQALPKELFGIRLPFPI
                     LSTTDDVNTILILSVIFGLIQILVGLFVAAKENIRRKDYLSAVSDGFAWQGILIGIVI
                     ALIGVMLIKNTAFVYLGAGLSIIAALCILIVPIIQSPSKVKGAAKGAYNLYGLTGYIG
                     DLVSYTRLMALGISGGSIGAAFNMLVAFMPPAARFSVGILLIIALHALNMFLTLLSAY
                     VHGARLQYVEFFGKFYTGGGRAFDPLKTAEKYVNINHKKKNNK"
     gene            73115..73585
                     /locus_tag="SCJ86_000310"
     CDS             73115..73585
                     /locus_tag="SCJ86_000310"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738015.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATP synthase subunit K"
                     /protein_id="HEG1167900.1"
                     /translation="MMDYLISQNGGMVFAVLAMATATIFSGIGSAKGVGMTGEAAAAL
                     TTSQPEKFGQALILQLLPGTQGLYGFVIAFLIFINLGNDMSVVQGLNFLGASLPIAFT
                     GLFSGIAQGKVAAAGIQILAKKPEHATKGIIFAAMVETYAILGFVISFLLVLNA"
     gene            73606..74190
                     /locus_tag="SCJ86_000311"
     CDS             73606..74190
                     /locus_tag="SCJ86_000311"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295097.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATPase V"
                     /protein_id="HEG1167901.1"
                     /translation="MNAIEKIISQMNEAAEQERAALEQEERMKIDQNFEQKRTQIETE
                     HQKQKEKQIELLEKKYRQLRNRQQVEVRQENLNAKQEFLRRLFADAIAEMENWDESEQ
                     IQFIKNSLYSLPLTGKVTFIAGEKSAAYLSQTLLDEWNNELPFMMVLSDETVADQAGF
                     LINDQGVQYNFLFSSLVQDIQGTMSFEIANQLFE"
     gene            74205..75191
                     /locus_tag="SCJ86_000312"
     CDS             74205..75191
                     /locus_tag="SCJ86_000312"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002308529.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATPase subunit"
                     /protein_id="HEG1167902.1"
                     /translation="MEYHELNPLIRGRELELLSNETFERMIQTNSVEALGEILKSTIY
                     SPYIYDGYEYDFEQNLLKEQSRLFRWLKESAPEPEIVWIYTMRYTFHNLKVLTKAEMT
                     NQNLDHLYIDDGFYSLETLKDAIHTQVSNELPESIMDSIREVRDYFEESTILQGIDVI
                     YDRQFLTEQRRLADKLGYQELLTEVIAFIDLTNITTMARGILQKRTYGFMTTVLSSSG
                     SIEKEVFLNFVDKDMEDYTNFLLETDYADFLRPVIREGNLDLVKLEQLKDDYLSSLYQ
                     EAQTQAFGPLPLLAFLNAKEVESKNLRLLVIGKRSHFEPETIRKRVRQVYDA"
     gene            75181..75492
                     /locus_tag="SCJ86_000313"
     CDS             75181..75492
                     /locus_tag="SCJ86_000313"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007209277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATP synthase subunit F"
                     /protein_id="HEG1167903.1"
                     /translation="MTHKIGVVGDKDSVLPFKLFGFDVRYGTTKQTVRQAIEEMAKNE
                     YGVIYVTEQCAALSPETIERYKEQMIPAIVLIPNHQGSLGIGLEEIQKNVEKAVGQNI
                     L"
     gene            75514..77295
                     /locus_tag="SCJ86_000314"
     CDS             75514..77295
                     /locus_tag="SCJ86_000314"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATP synthase subunit A"
                     /protein_id="HEG1167904.1"
                     /translation="MQIGKIIKVSGPLVMAENMSDASIQDMCLVGDLGVIGEIIEMRG
                     DVASIQVYEETSGIGPGEPVRSTGEALSVELGPGIISQMFDGIQRPLDTFMEITQSNF
                     LGRGVQLPALDHEKKWWFEPTVEAGETVSAGDVIGIVEETKVIKHKIMVPNGIKGTIK
                     TIEKGSFTIDETVCVIETENGDKNLTMLQKWPVRRSRPIKEKLNPDAPMITGQRVIDT
                     FFPVTKGGAAAVPGPFGAGKTVVQHQIAKWADVDMVVYVGCGERGNEMTDVLNEFPEL
                     IDPNTGESLMERTVLIANTSNMPVAAREASIYTGITIAEYFRDMGYDVAIMADSTSRW
                     AEALREMSGRLEEMPGDEGYPAYLGSRLAEYYERSGRVIALGSEGREGSITAISAVSP
                     SGGDISEPVTQNTLRVVKVFWGLDSSLAQKRHFPSIDWIQSYSLYSTEVGKYMDHILQ
                     ADWASMVTEGMRILQEEQQLNEIVRLVGIDSLSDNDRLTLEVAKSIREDYLQQNAFDD
                     VDTFTSREKQFKMLKMILTFGEEGRRALSLGAYLSEIMDGTVSIRERIGRSKYIPETE
                     LEKLDQIIGEIKQTMQTIVSEGGMSND"
     gene            77288..78664
                     /locus_tag="SCJ86_000315"
     CDS             77288..78664
                     /locus_tag="SCJ86_000315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629451.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATP synthase subunit B"
                     /protein_id="HEG1167905.1"
                     /translation="MIKEYRTIGEVVGPLMAVEKVAGVKYEELIEVRMQNGEIRRGQV
                     LEVQEDKAMVQIFEGTSGINLRDSSVRFLGHPLELGVSEDMIGRVFDGLGRPKDNGPE
                     ILPEKHLDINGEVINPVARDYPDEFIQTGISAIDHLNTLVRGQKLPVFSGSGLPHKEL
                     AAQIARQATVLDSSDDFAVVFAAIGITFEEAEFFMEDFRQTGAIDRSVMFMNLANDPA
                     IERIATPRMALTAAEYLAYEKGMHVLVIMTDMTNYAEALREISAARREVPGRRGYPGY
                     LYTNLATLFERAGRIRGLKGSVTQIPILTMPEDDKTHPIPDLTGYITEGQIILTRELY
                     KSGIQPPIDVLPSLSRLKDKGTGPGKTREDHAATMNQLFAAYAQGKQAKELAVVLGES
                     ALSDVDKIYAKFAERFEKEYVNQGFYTNRSITETLDLGWELLSMLPRTELKRIKDDML
                     DKYLPEGK"
     gene            78671..79306
                     /locus_tag="SCJ86_000316"
     CDS             78671..79306
                     /locus_tag="SCJ86_000316"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346470.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATP synthase subunit D"
                     /protein_id="HEG1167906.1"
                     /translation="MARLNVNPTRMELTRLKKQLTTATRGHKLLKDKQDELMRQFILL
                     IRKNNQLRQEMEESMTEAMSDFVLANASITEPFIEELFVLPAENVDLSIVDKNIMSVK
                     VPVMNFEYDEQLTDTPLEYGYLNSSAELDRSIDRFTTLLPKLLELTEIEKTCQLMASE
                     IEKTRRRVNALEYMTIPQLEETIYYIRMKLEENERAEVTRLIKVKNMGTTD"
     gene            79336..80691
                     /locus_tag="SCJ86_000317"
     CDS             79336..80691
                     /locus_tag="SCJ86_000317"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738846.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Trk family potassium uptake protein"
                     /protein_id="HEG1167907.1"
                     /translation="MIKQSKQIKKRLPAVQLLALGFFLLILVGGGLLTLPFFSNSGHG
                     TNFIDALFTATSAVCVTGLTTLNTAEHWNSAGQFLIMLLIEIGGLGFMMVPILCFALI
                     KKKVSLSTRIILKEALNLEEVSGVMKLMLYILKLAFVIQLIGTVALSFVFVPEFGWLK
                     GIWYSLFHAISSFCNAGFDLLGDSLANHQQNVYLIMVVSALIISGGLGFIVWRDLLEY
                     HQKKKMTIHSKIALTVTGTLLLGGFVVFFFTERNGATLVEGNMFQRLANTFFMSVTPR
                     TAGYYSVDYLHMSHAGLILTMFLMYIGGTSGSTAGGLKTTTLGVLLIQMRSMLKGRTR
                     AEAFGRTIRQGAVLRALTLFFVTLTLCVVAIMILSVTETIPKTSGIEYIAFEVFSAFG
                     TVGLTMGLTPDLTAIGKLIIIALMYIGRVGVLTVVFSLMTKAQRTQSKYKYPEESVMI
                     G"
     gene            complement(80883..82277)
                     /locus_tag="SCJ86_000318"
     CDS             complement(80883..82277)
                     /locus_tag="SCJ86_000318"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334118.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PLP-dependent aminotransferase family protein"
                     /protein_id="HEG1167908.1"
                     /translation="MPINSYDNYPLTWRPDKQKLKRPYYRSLIQQLEQDILKNKLQKN
                     TQLPSQRELADYLDLNFTTVGKAYKYGIEKGLLYTNIGSGTFISPNVFESITISTNEV
                     TEHIIDFGLVSSFEECNKFVVPFIQSTSNNPNLVGLLNYQDPKGTNFQLTTAANWLKT
                     QGVIPSLESLSIVSGVQNGLAITLSALFSPGDRIATDRYTYANFIELAQLYHLEIVPI
                     DFDEQGMRPDILIQECRKKKIHGVFLMPSCNNPIGFQISHKRREQLAEVIEKEGLWVI
                     EDDIHSFLTTYYQHTIIPPFQNLLPNQTIYLAGMTKFICSGLRVAYLVVPVHVQSKIE
                     QAIFNINVKTSGLDVEVITQILHSNEANRILETKLSLTKRANQLFDSLFKVEKPTNRV
                     PYYRILPVDPEIDPKIIENDFLANGIRLYHSRRFTTRAQPDAFVRISLSSNNLEILAS
                     GLSIVKEKIKKYEL"
     gene            82369..83340
                     /locus_tag="SCJ86_000319"
     CDS             82369..83340
                     /locus_tag="SCJ86_000319"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ornithine cyclodeaminase family protein"
                     /protein_id="HEG1167909.1"
                     /translation="MKIINFEEVVASFSFEEAIQVMKKCFFDYESGKISQNPRLVEIL
                     PDGKGKNMFAMMPAYLGENRYFGTKVITAFPDNHQYHLPSHLGEIFLFDAQNGLPKAL
                     INANAVTWIRTAAVSALATDYLANPHAETLALIGAGQQALSHLTAISTIRPITTVFVY
                     DILEERRNTFIAKAKKQYPKITFINAISVEDAVRPAEIICTLTSSKEAFLQKEWVRED
                     AHINAVGTFTPDTREISTSLMETSQIYVDDYSAALTESGDLLIAMAEGDLEESCIVGT
                     LGELVSEKKTFYKGESRPTIFDAVGLAIEDLCCAEYIYNKLEKGALK"
     gene            83337..84119
                     /locus_tag="SCJ86_000320"
     CDS             83337..84119
                     /locus_tag="SCJ86_000320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295114.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PhzF family phenazine biosynthesis protein"
                     /protein_id="HEG1167910.1"
                     /translation="MNLDYYVVDAFADEVFKGNPAAVYVLEEWLPEGTMQKIAIENNL
                     SETAFTVKKNQEFELRWFTPDREIDLCGHATLATAFVLFNYYKIPDETIKFSTQSGNL
                     FVTRIKDYYYMDFPSIMPKKVPILSEYEEAIGAKIKEAYLARDLFFVLEDEKTVAKLQ
                     PDFTAIKNFELGIGVIVTAESVQKDFVSRTFFPKLTINEDPVCGSAHSNLIPYWAERM
                     NKTKLSAYQMSSRGGDLECELKEDRVIIGGKAVLFSKGTSFI"
     gene            complement(84288..84659)
                     /locus_tag="SCJ86_000321"
     CDS             complement(84288..84659)
                     /locus_tag="SCJ86_000321"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010752563.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HEG1167911.1"
                     /translation="MKMAHTCVRVKDLEASIEFYQKAFGFEESRRRDFPENKFTLVYL
                     TLPGDDYELELTYNYDHGAYDLGDGYGHIAIAADDLEKLHEQHKAAGLEITDLKGLPG
                     TAPSYYFVIDPDGYKIEVIRG"
     gene            84793..85236
                     /locus_tag="SCJ86_000322"
     CDS             84793..85236
                     /locus_tag="SCJ86_000322"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325070.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX domain-containing protein"
                     /protein_id="HEG1167912.1"
                     /translation="MDKPIFGEKKQFADYQTRYAAYIVIERDQQIAVIEAPNGAFFLP
                     GGEIEGEETKEEAIVRELIEEMGIVAEISSYLGEADEYFYSNYRATYYYNPGYFFVAD
                     SWKRIGEPTEKTNKIWWVSPQEALVKLKRGSHRWAVERWIEKNKN"
     gene            complement(85279..86088)
                     /gene="yidA"
                     /locus_tag="SCJ86_000323"
     CDS             complement(85279..86088)
                     /gene="yidA"
                     /locus_tag="SCJ86_000323"
                     /EC_number="3.1.3.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar-phosphatase"
                     /protein_id="HEG1167913.1"
                     /translation="MSIKLVAIDIDGTLLNEKREVTKEVKEAIAAAVAKGVSIVLCTG
                     RPLPGVQEQLNELNLFQDNDYVITYNGALVQQTKSGKIIARHGLTHEDFLEIEVMARR
                     VGSHLHSIDDKAIYTPNRDISAYTIHEASLVKMPLKYRTPEEMTPDMNIVKMMMIDEP
                     EILDAAIARLPQTFRDKYTTVKSAPYYFEVLNKEASKGAAVANLAQHLGIDQDEIMAI
                     GDNENDLSMIEYAGLGVAMGNAVSLVKEAANVITSSNDEHGVAEAIKKYVL"
     gene            complement(86107..87474)
                     /locus_tag="SCJ86_000324"
     CDS             complement(86107..87474)
                     /locus_tag="SCJ86_000324"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629448.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing protein"
                     /protein_id="HEG1167914.1"
                     /translation="MTTPYKYQMLPMEKVFRDPVHNYIHVQHQVILDLINSKEVQRLR
                     RIKQLGTSSFTFHGAEHSRFTHSLGVYEISRRICDIFSRNFSKEKIGNGGWDDQERLV
                     TLCAALLHDVGHGPYSHTFEHIFHTNHEAITVEIITSPETEVYQILNRVEEGFPEKVA
                     SVIQKTYPNPQVVQMISSQIDADRMDYLLRDAYFTGTEYGTFDLTRILRVIRPYEGGI
                     AFSMSGMHAVEDYIVSRYQMYVQVYFHPVSRGMEVILEHLLHRAHELYQEDASSFELH
                     SQLLLPFLDGSFTLEEYLKLDDGVLGTYFTQWSESADPILNDLAKRFLNRKPLKSATF
                     SGNRDSKLVQELTLLVEKVGYNPVYYTAVNSSYDLPYDFYRPEQGRHRTQIEILRNDG
                     TLIELSQVSQLVAALAGQEQGDERFFFPKEMVDPSLRDHYDLFDETYQEFASHIRNGA
                     LIEIN"
     gene            complement(87458..88288)
                     /locus_tag="SCJ86_000325"
     CDS             complement(87458..88288)
                     /locus_tag="SCJ86_000325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377477.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoate--protein ligase family protein"
                     /protein_id="HEG1167915.1"
                     /translation="MNPAIRLDQHIYQKNDFSPFALTDILTEYSKEHQQIFLHFWQYD
                     CTVILGMKDVRTPFLSDGIQTLKDAGYAPVIRNSGGLGIVSDEGILNISLIFPQSDTK
                     KITIDDAYEQMLLLTKYAFPKAPIQAFEITHSYCPGTYDLSIHGKKFAGIAQRRIKNG
                     ISVMMYLSVNGNQEKRGQVMKRFYQSALKEDFGTNGYPAVDPATMATLEELLDQPLFV
                     KEVKEAFAKAFEKLYSASDLQDSTDWLKKEIQLEQWNNQIQRMEQRNNIKELHYDNPL
                     "
     gene            88451..88888
                     /locus_tag="SCJ86_000326"
     CDS             88451..88888
                     /locus_tag="SCJ86_000326"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292826.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1934 domain-containing protein"
                     /protein_id="HEG1167916.1"
                     /translation="MELSQGTPISIKLRTKVKQQGEVQDFYFDLKGQMVTIGDTLYIR
                     YKEIQEETGDEIPVTIKLVPDGHVQLIRAGEMRMRLKFGYKERLETSYRTPYGMIQIA
                     TFTKELHVSLKDRPTGGKVRIDYDLFMGPEKIGEYYLTLDFTA"
     gene            88964..89584
                     /locus_tag="SCJ86_000327"
     CDS             88964..89584
                     /locus_tag="SCJ86_000327"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304561.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit delta"
                     /protein_id="HEG1167917.1"
                     /translation="MEISVFEGANKSELSMIEVAHAILEQRGDVMDFSDLANQIQNYL
                     GKSDSEIRDSLAQFYTDLNIDGSFISLGDNRWGLRSWYAIDSIDEEVNHGMDEEDEDT
                     PRRRKRKKVNAFINDDEDAIDYNDDDPEDTDLTEEDDDDLFDDDDDEDEEIAAYNSDL
                     QEIGADDPTDDEEDLPGNIEEDLSIIDEDDDDEDYEEEEFSDSEKE"
BASE COUNT        29552 a        15170 c        18471 g        26460 t
ORIGIN      
        1 ttaaataaat aggaacaaaa aaattaaatt atttaataca cacattaggg atcattcatt
       61 gaaggattag aagacaacga ggatggcgaa aaatatgatt acaatagaaa agtcgttcta
      121 aaccaatcag ttcctagtat cacacaacgc aagccagtcg atttggtgag attagaggag
      181 tgaaagcaaa ctttattata taagaaaaaa tattcgaaag ggatgctagt ttaggtacta
      241 gtgctgatag attaatatga tgcctcgtaa tattaggcgt agttactcta gagacatcac
      301 tatggactat gatttgtctc agagtcacaa catgtttttt tatgaaaaga aacagctaaa
      361 ataattaaat tgcgcaatgt ttaaactttc ttaaactata acctaataaa tgtgaagaaa
      421 taatgcacaa tttcttcaca tttatcttct attctatata cataccaacc aacaacccta
      481 tatacttttt catttttact cctcaagtaa aaataactcc tagaccgcgg ctacccaccg
      541 cggtcgtttt tttattcttc aatcaaaatc gctttgatac ccaaacctgc caagattcgg
      601 gcagaagtat aagtattcaa tcctcttctg aaagtaatgt aaactttttt atcttttggg
      661 atttcattga ttcgatctcg taactcattc attggaatat tttttgttgc cgagatactg
      721 ccagaaggtg ctttattagg ttcacgtata tctaaaaaga acgcttcttt caaatcgttt
      781 tcgggtatat cacttgcttt gatcgtttct acgatattcg tcatttgatt gattgcgaca
      841 taaccagcta tattcaacgg gtcacgggta gtagaatacg gtggagagta aggcaactcc
      901 aaatcaggca aatcaaagac tgtcaagttt ccggtgattg ctacagaaag ttctcccatc
      961 cgtttatcga cacctttttc tccaaatgct tgtccaccta aaatacgacc gctatctgca
     1021 tcaaaaatca gctttagatt taatcttgta gcttctggat agaaataggc atggtcaaag
     1081 ggagtgataa aaacagtttt ataatttgtt atgcctgatg cttttaattc atgttctgtc
     1141 attcccgcat aacttgctgt atgatcaaag atttttgcga cacctgcacc aatatatccg
     1201 cgatagcgca taggcgtacc atttaaaata tctgccagca tatgtccttg acgattggcc
     1261 gcactggaaa gcatactttg gatcggctga ccagtcacta cactagtcgt ttcgataata
     1321 tcgccaatcg catagatgtc cggcagattg gtttgtaact ggtcatcaac aatgatctga
     1381 cctgtatctg ataattgtat gccggttgct ttcaccactt cattcttcgg agaaacacca
     1441 acagcaaaaa ttagcatatc agcagaaagg acggagccat ctgataatac tatttctcga
     1501 cctttatcac ggatctcagt aactcttgtt tctaaatgaa cggctaatcc ttctttcagc
     1561 aatttgtcat aaactaaatt agcaatttct ggatcataag ggaaagcgac ttgtggcaat
     1621 tgatcgatta aagtcacgtc taagccgcga tgcttgaaat tctcagctaa ttccagaccc
     1681 ataacacctg caccaagaat gacgacatgt tgtggatttt tttcttcaag gaacgatttg
     1741 attcgatccg catccgtcac actacggagg acaaagccgt tttctgcttc aaaaactccg
     1801 ggaatatccg gataatctgg tctagcacca gaagagatga tcaacttatc gtaatctgtt
     1861 tttatttctt catttgtgga taggtcttta actgttaatc gcttagtaga aggatcgatt
     1921 gctgttactt cgtgtttagt aaagacatcg atgttgtttt ttgttttcaa tatttctggt
     1981 gtacgttcga tcagcgagtc acggtcagtg atcacacctc ctaaataata aggcaaagca
     2041 caacttgcat aagaaatatt ttctccgcgt tcatagataa tgatttcgtg cgcttcattc
     2101 aatcgacgga aacgagtggc aaatgaagga ccgccagcta caccgccaat gacaacaact
     2161 ttcataaaaa catctccttt tctacgttga tcgtactatg ttatggtaaa aaacagcaaa
     2221 taattgtgcc ggaaaattat tgtttatttt gaatagacag aaaatataga gaaataattt
     2281 gtggataaaa cctaaaaatt tttttctatt ttaaggatag ataatacttt gaaggggata
     2341 accctgtata ttttttaaaa gctgaagaaa aatgatggat cgaatgaaat cctaatatat
     2401 ctgcaatttc tgtataggtt aattcatttt ctagcacgag acgttttgct ttttccattt
     2461 ttagttcttt aaaatagcta ataacacttt ttccattcac ttttctaaaa tgttttttta
     2521 agctggctgg actgataaaa aaaatttggg ctaattgttc aagcaaaaaa ttttctgaaa
     2581 gatgttcttc taaatatcgt atgattggat caactaattc atctgaataa agaatatgct
     2641 tttttttagg aatgactttc tcatttgaag gtattgtcaa taaagcaagt tcctgttgat
     2701 aatgccttgg taaggaagac aattgaattt tttgcagaca ataagtaact aataaggaaa
     2761 gcaaatgaat gctcatctgt tggcatagta tgaaaaaaga attgatttcc tgatcatagg
     2821 aaatagctgg cttactatgt tttagataat caattttgaa ctgttcattt ttcagaaaag
     2881 taatgataga tgttatttct tgatctatag aaaaagattg tcccgtaata tgtgctaata
     2941 attgctcatc aaaataaaac tggataatga taaaagtttg tgtttccttt tgagtagaaa
     3001 agatttggac ttcttgctgt ttatcaatga ataaaaggga aggattatct agttctatag
     3061 atttatgtgc aaattcagca tataaattag cgctttcatt tattagtagt cgaaaatagg
     3121 gagcagcaga taaatgaaga tgggtatcta tggaataggt tagtgttttt ttgacaagat
     3181 tggaggattg gttgtttgtt aacaaaagaa ccactttcct ttctcttttt atataaaaag
     3241 ctttctattt ataattaatg actattataa caaaaatgaa aagccaaaac gacaagtaaa
     3301 atattttttt atataaatat ttgtgttact aattccgtta atatacataa gtgaaagcgt
     3361 tttatataag aacttgaaaa gaaagaaggg atagacatga aaaaaagata caaattggtg
     3421 atagctttgc taaacattac cctattattc agcttaagtg catgtggaaa aactaacgaa
     3481 gaagtaacag caaacatcgc agatccaatg ataaaaaaag ccactacatt agaagtatat
     3541 gtcgcaaacg gcaaactgac aaactatgtt gaaaatgctg caaaaatcta taaccgcaaa
     3601 actggagcaa atattgactt aaaaattgtg aatgtagctt cgggaacagc aacagtacaa
     3661 atgatcaccc caaaattggt aacacacgaa gatatgccgg atattatctc catggcagac
     3721 acttcagcag caggtgtgct ggaaaaattt caagatgctt tttatagtgc tgatgaatat
     3781 ggattttatg acaaatatgg cgatcagttt tatgatcaaa aattgaatat tttgcgtcaa
     3841 caaacgagta atgaaacgat tattccttgg gctagtgatt ttaccccagc actttgttat
     3901 tatcaaccaa aaaaatttgc tgaaataggt gtagattttg aagaaatcag ttcttgggaa
     3961 gaatatatcc aggtggcaaa agcattaaaa gaaaaaactg gtagttttgg aattgccttg
     4021 ccagaatctg gggaccaaga attgtttatc aacatgctag cccagcagaa tcaatctttg
     4081 cttgatgaag aaggaaatat ccgtctgaat actaaagaag cgaaacatgc tgcaaagtta
     4141 atcaaagaaa tgatcgactc agatattgta catttctacg gtgcacaaga tgctgagaaa
     4201 gcatttcaag aatctgcaat gtttgttgct ggcggctggt atgctacgaa tatgtctttg
     4261 aattttcctg atgcatctga caaatggcgc atggctccac tgattccttt ttcaaaagaa
     4321 aatccaggaa aaggtgcagt atcaggagga tcaagttttt atgtccctaa agatgccaaa
     4381 aatccacaag tggcacaaca atttcttaca tttatgctga cagatgaaga atgcttagcc
     4441 aacgcactgg agctgggcgt ggctacatct aactcattgg cctatcaaac agaagcagct
     4501 gagaaaaagt ttgactacta aggaggacaa aaatactatg aactattgaa taaaatcaat
     4561 gaaaatacat caaatactgt tttaccagcc acttattcag atgctacgtc ttatttgact
     4621 accgctgcat ataactattg gcaaacaggt gattttgaaa attcatatct aaaagaagca
     4681 gaaaattttg ctcataagta cggcgtaaaa ttaactggtg attaaaaaat attaggagga
     4741 cttaacgatg atgcaatctt cgttaccaag caagacaata aagaacagca agaaaaaagc
     4801 aacagatacg tctcataaaa aaagtgctta tggattggtc acaccttttg ttattttctt
     4861 tattgtgacg attttgattc ccatcattat ctcgtttgct tttagtttta tgaaaattgg
     4921 ctactcattt gaatttgttg gatttgacaa ttatctagct atcttccgag atccattgtt
     4981 tattaaatct tacacgaatg ttttgattat catggcagga tcaattccaa taacgatgct
     5041 attagctatt tactttgctg ttttattgaa ttcagcacag ttaaaaggaa aaggattttt
     5101 tagaactgtc tattatattc caaccattac atcaacggtg gctgtagctt ccgtattcat
     5161 gacttttttc aatccgactg ggttattcaa tggatttttg aatgcgctag gaattaacgg
     5221 tgtacagtgg ttaacaaatc ctttttggat acgaatatca atgatcatca cgatgatttg
     5281 gcttagtgta ggatataaca ctattttatt tttagctggt cttcaaggga tttctaaaga
     5341 gatatacgaa agtgctgaaa tagatggagc atcgaaattt cggcaattct ttttattaac
     5401 gatccccatg ctaaagccaa tcattttaat ggccacagtt ttagccacta tcaatggctt
     5461 gggtaatttc aatatcccaa atatattctt tgggacttcc aatggaccag aaaattcagc
     5521 tttggtcgta ggagtcaatt tatataaaac aagttttgaa atggttgatt ttggaaaagc
     5581 ctctgctatt gcctggacga tggtgtttgt atctgttata ttatcggcta ttcaatttaa
     5641 attcggaggt gataaaaatg actaaacaaa agatacaaag cagaattttt acgtacgtca
     5701 ttattgtgtt tggtgcctta atatttttga tgccatattt atatatgatt gtagcttcaa
     5761 cgcaaagtaa tcaagaaatt ttaagtggcg gaatcaattt caagtttggg gatatgctat
     5821 taaaaaactg gcaagccatc atggcgagat ataattatcc cttgattatt tggaattcga
     5881 ttgttattgc tgtaatcagt acagttttag ctacagccag ttctacaatg gcgggttatg
     5941 cattagcaaa gtatcgtttt atggggaata aactgttgtt caatacagtt atgctatcta
     6001 ggatggtgcc tttttttacc acattgattc ctttatttta cattatgagt cgtttaggat
     6061 tatcgaatac ttatgcaggt gtgatcatcc cttcgattgc cagtacaact tcagtgttta
     6121 tgatgcgaca atatagcagt caatttccta cagcactgat ggaggctgcc agaatcgatg
     6181 gtgccaatga gtgggtgatt tttacgaaaa tcgctgtacc gattttatca cctacgatta
     6241 ttactagtgg tttacttatc tttatgggaa gttggaatca atatctattt ccattagtca
     6301 tgctttcaga taaagataaa tatacggttc ccttaattat caaaaatatg acggtttcta
     6361 gtacaggtga gccaattaat tatggcgcaa ttatgctaat tttagcaact tcagtgatcc
     6421 caatggtctt gatttatgtg tgggcgcagt ctaaatttaa ggagaatgac attggagctg
     6481 caaataagta aaaaaaataa cgaatgttaa ccatctagtt cgttctagga gcaactccac
     6541 attcataaaa attaaaagaa tggcgggata tccatgaatg aacagacgaa ggaatggtca
     6601 agaaaaattt tgaatgtagc ttctgaaaag atagatgcgg aattagacag aatggggaat
     6661 aaaattccat atatttctta caacggaaaa tatacagacg taataaagga cagaggaatc
     6721 gactggtgga ccaatggttt ttggagtggt attttatggc agctctttca caagacaaaa
     6781 cgagaaaagt tcaaagaggc agcaatacaa caagaacaat tattagaatc tgccctgtac
     6841 catttttcag acgttcatca tgatgtaggt tttttattct taccatcggc tattgcacat
     6901 tatcaacagt ctcaaaacaa acatagtcga caactagggt tacatgcagc tacggtactt
     6961 gctggacgct ataatatttc agggaactat ttagtagcat ggaatgacca taaacctggt
     7021 tggatgatta ttgatagtat gatgaattta ccattactgt attgggcttc gagggaaact
     7081 ggtgatccga ggttctctaa aattgctcgg cagcatgcag atacaacagc taagtatcta
     7141 gtgaggaaag atggatcaac cgggcatatc gcagaatttg atgcagaatc agggaaatat
     7201 attcggttac ttggcgggca aggatacaat gagaattctt gttggagcag aggaaatgca
     7261 tgggctttat atggattcgc gttaagttat cgttacacaa aaaatccgta ctatctagct
     7321 atagcagaaa agatagcagt aaattttatt cagaaaatcg aaaaaactgg ttatattcct
     7381 ctcattgatt ttgctgtaaa agattcgaat attgatacca gtgctggagt atgcgctgca
     7441 tgcggtcttc tggaattagc aggatatgtt tcctctgtac gatcaaaaat gtatcatgaa
     7501 gctgctatca atatactcca aaagacagaa aagcactatg caaattggaa tatggatgaa
     7561 gatgggatca ttggaggcgg agcagaagca taccatagag aactgactta taatgtgcca
     7621 ctgatctatt ctgattattt ttttattgaa ggcttacttc gattagaaca gtcggaatta
     7681 gctatttggt agtaaaaaga ggagaaaata caatgaaaca tccagtacca ctattaagga
     7741 ccagacagga ttactttgac ttatttaaaa agataaatca accactccag ccgttttatc
     7801 gagcaagtgg cgcacaaatt gattatatac atcaaggggt aggatatgga aataaaattg
     7861 caggaatgga aggattctct cgggttttat ggggagcggg gcccgcaatt gaccaattag
     7921 atgaacgatg gttgaaatta attctatcag gaataaaagc tggaacagat ccttcccatc
     7981 ccgattattg gggagaaata catgaaagag accagcgtat ggtagaaatg cccgcaatgt
     8041 tattagcgtt atatcatcat gaacaattat tatggaaaaa attagataca ggagaaaaaa
     8101 gccagattat tcattggttc tcgcaaattt ttgaatatga atgtgcagat ggtaattggc
     8161 aattttttaa gataattgtc ggaacaatta tccaaaaatt tggttatcca gttaaagaat
     8221 cagcactgaa agaagcattt gataatatag aaaattgtta tcttcaaaat ggctggtatc
     8281 gagacagttc aagaggtcgc caagattatt ataatccttt tgcatttcat tattatggtt
     8341 tgatttatag cgtactagaa ccagaaaagc ccattagcca gatatataag aaccgagcta
     8401 ttgatttttc tagagactat attcattttt ttgcggaaga tggtgcaaat gtgccttttg
     8461 gtcgaagcat gatttatcgt tttgcagttt caagcttttg gtcagctatg ttgtgggccg
     8521 atcttttgcc ttttaaatta ggtgaaatca aggggttagt caatcggaat ataagatggt
     8581 ggatgcaaaa agaaatattt gatgaaaaag gtatcctgaa tattggttat acctaccctc
     8641 aattgacatt gactgaaccc tataatagta cgttatctcc attatggctg aataagattt
     8701 ttttattgtt ggcgctacct gaagaccatg agtattggac ggctaaagaa atgcctatgc
     8761 ctattcgtga aggaactaaa cttctttcag aagccaattt gctcatacaa catgataatg
     8821 gacatacatt ttttttgaat gcgggacaat tagggccaaa tttccataca ttgaccaatg
     8881 aaaaatattt aaaatttgct tattctagtc aatttggttt ttcgataccg cgagcgaatc
     8941 aattgaaagc agaaatggca atggattcaa tgctagggat tcagcgagcg gacacatata
     9001 tcacgaccag ttttaagaat gagagcatca aaacctatgg acagttcatc gttcgaaata
     9061 atgtacgaga cattgtatgt aagaaagagg tacttgcctc tacatgggaa cctactttac
     9121 aggcaaaaat cagaacttgg cttattcctt ttgaaggctg gcagattcgc gtacacaaag
     9181 tagaattaga tacagaatat gttctatatg agacagggtt tgctattgaa tgttctcctg
     9241 agaaagaagg aataatcata gaagaagaca aggaaaacta tagggtgagt gaagctggct
     9301 tttcaggtat tatctgttta agtgacgata ctattaaacg aaaatccaca gctattatgg
     9361 gtttgcccaa tacaaacttg atgacatggg aaaacacttt tattcctgga ttagagggta
     9421 catttggaaa aggaacccat tggctcggaa caggagtggt tgctcatcaa gatcgggatt
     9481 atagtttaga agtatggaac aatcgtccca aaattgattt tgatgggaca accgggctaa
     9541 ctatccaaaa taaaaacaat aaaaaaagac taaaattatg ctaaaggctg gtggggaaat
     9601 gcttgataaa gtgatgtttg aagagataat acatccagaa aaatggataa atgaatgtta
     9661 taaagaatta atggaaaaag aatggaataa tgagatcgag cgttgtcgca gacacacgga
     9721 gaggttaatc aaacgtatca gtaacagtca gtatcaattc ccaagcgagt gtcctataaa
     9781 aggacgttat ccattaagag acaatattgg ttggacagaa ggattttgga caggaattaa
     9841 ttggttgtta tttgagaaga cgggtgatcc tttatttcaa caaacagctg aaaaagcaga
     9901 tatagatttt atccacagga ttgactgtca aattatcact aatcatcacg atcttggttt
     9961 tttgtattct ttatcaaccg tagcagatta taaaataacg ggaaatcgaa aaatgaagga
    10021 agcttcaatt gatgctgcag aaacattact tcagcgtttt tgggagaagg caggagttat
    10081 acaagcttgg ggagatccta gtgatccttc acaacaaggt agaatgatca tggattgttg
    10141 catgaatctt ccattattgt attgggcgag tgaagaaact ggaaatcaaa aatataaaaa
    10201 agctgctcag gctcatattc aaaatgcagc agattattta gtaagagaag atggttcaac
    10261 ttatcataca ttttatatgg atgtcaaaga tggaaaacca agatatggaa gtacgcatca
    10321 aggttatagt gataattcct gttgggctag aggccaagca tgggggattt acggttttgc
    10381 attaagttat cgttatttga aagaacaaaa atattttgat ttaagcaaaa tattagccaa
    10441 ttattttatg aatcggttgc cagaagatac catttgttat tgggatttga tctttgatga
    10501 ttctagcaga gaagaaaaag atacatctag tatggtgatc atggtttgtg gattattaga
    10561 attaatagaa tcaggtctat tagacaaaac agaaaagaac ttataccagt ctgctgtttg
    10621 gaaaattatg cgacaattga aagaaagata tgctacaacg gaagaagaag atggcttttt
    10681 aacacagtct gtttataata agcccaaaaa taatggggta aatacacctt gtatttgggg
    10741 tgattactat tatttagaag ctttagtgag aatgactagt aattggtctt cttattggtg
    10801 aaaaaggtaa atatggaatt tttgtttgat aaaataaaaa aagaaaaacc aaaacagcat
    10861 gaatggtttc tgttttggtt ttttcgttaa ttttcatatt cttctaattc caagcttagt
    10921 tcttcccatt ccgtcaattt tgtttcttga tcttgtctag cagcttctaa atcttggttc
    10981 agctcattga gttttacgtg atcttccagg ttttctggcg cgaccatttc ttgttccaac
    11041 tgatcgatcc attcatcgat ccgcgccatt tcttcttcaa cctgagtaat ttttcgttga
    11101 aggctacgga taagcttttg ctgttctttg ctttggtaat aatcagattt gttcgtggga
    11161 gcaggagcag attctggctc atcttgtgcc aacaattcgg cgatttcttc ttcttcctgc
    11221 tttttctcta aataataatc atagtctcct agatagactt tgctgccatt ttcagagagt
    11281 tcgacgactt ttgtcgcgat ccgattgatg aaatatcggt catgggaaac aaaaagcagt
    11341 gtgccgtcat aatcgatcaa tgcattttct aatacttctt tgttgtcaat atctaaatgg
    11401 tttgttggtt catccaaaat caaaaaattt tctttgttca tcgctaactt ggctaaagca
    11461 acccgtgctt tttcaccgcc acttagcaga gggattggtt tttctacatc ttcgccagaa
    11521 aataagaaag cacctaaagc acttcggatc tctttttctg gagtggtagg atgctcatcc
    11581 cacaattctg ctaggatcgt cttgtttgta tgcagatcag cttgaccttg atcgtaatag
    11641 ccgatggtga catttgtccc tagcgtttct tttccacgga taaaagggat ctggtgaatg
    11701 attgatttca gcaacgtcga tttcccgata ccattcggac ctactagagc aatcgcttct
    11761 tgacgacgga tatctagatt gattggttca gataaggtga cattatcata tccgatagcg
    11821 gcatcttcga tttgcaagac tacatttcca gaaggctttt ctatactgaa tagaaagttt
    11881 gcttgttttt catccccttg aggacgatcc agccggtcca ttttttctaa tgtctttctg
    11941 cggctttgcg ctcgttttgt ggtagaggcg cgaacaaggt tccgagcaac gaagtcttct
    12001 aatttatgga tttctgtttg ctgcttttca taagccttcc attcacttgc taactgttct
    12061 gctttcaaat ctagatattt cgaatagtta cctttgtaat ggcgcatttt tcttctgctt
    12121 aattcataga cttcattgac tactttatct aaaaaataac ggtcatggga aacaatcaat
    12181 aatgcaccag tatagctttg cagataattc tcaagccagg aaagtgtctc gatatctaaa
    12241 tggttcgttg gttcatctag aatcaaaatg tcagggtgtt gcaaaagcat gcgagctaaa
    12301 gctaaacgtg tgcgctgtcc gcctgataaa gtatcgattt tctgatcata aaatgaagaa
    12361 tcgaatttaa acccatgcaa tacggaacga atttcatttt cataaccata gccgtttcga
    12421 tctgaaaaat cgtgttgcag ccggtcgtat tcttttaaat aagaactata agcaggtgtg
    12481 tctggcgtac cttcgctaat gaaaatctct agctcgcgca tccgctcttc catttgtcta
    12541 acttgttcaa aagctttcag catctcgttc caaactgttt cattggaatc aagtcctgtg
    12601 tcctgagcga ggtatccgag agtcgctgtt ttatttttag cgatctttcc ttcatcaggt
    12661 gcttcaattc cagcaatgat ctttaataaa gtcgattttc cagcgccatt tctgccaact
    12721 aaagcgacac gagcgcctgt actgatttct aaatgtatgt tttcaaataa gacttctgca
    12781 ccaaaatggc gagcaatctg attggcttgt aataatatca tggaatcctc ttttctactt
    12841 tgtgaattct cctcaaataa aaaactattt ttcaattagt agtctaccat aaattccatt
    12901 atactgcgag atttgcagaa atgataaata aatgaataaa gaataaaatt tcacaatatc
    12961 tgagcaatgg aattgatttt ttcctataaa tactgctatg atggagaaat caaagatgta
    13021 attagacaat acgtggaggt catttcgtga aagatcatat tattccaaag gcgacagcta
    13081 aaagactgcc tttgtattat cgctatttaa ggattttaaa taatgctgga aagaaaaaag
    13141 tgtcttcaac tgaattaagt gaagccgttc aagtagatag tgcaacgatc cgtcgagatt
    13201 tttcttattt tggcgaatta ggaaaacgtg ggtatggtta tgatgtagaa gatcttatgc
    13261 acttttttgc aaagacatta aacgacgatc aattgacaaa tgttgcgctg atcggtgtag
    13321 ggaacttggg aagtgcttta ctaaaataca aattccatca aagtaatagc atacgtatca
    13381 gttgtgcttt tgatgtgaat gaggaaatcg ttgggcgcat cgtagacggt atccccgttt
    13441 atccaatgga agatatgtca gaacaaatca agatccaaca aatcgaagta gcaattttaa
    13501 cgatacccgc tgaaagagca caagaagtag tcaatcagtt aacggaagca ggaatcaaag
    13561 gaatcttgaa ctttaccgct gctcgattga atgctccgtc agatgtattg atccaaaatg
    13621 ttgatttgac gaatgaattg caaaccttga tctatttctt gcattctgac gcaaatgcgc
    13681 attccgaaaa tacagcagaa taaaaataaa aagcgattga acaaatgaag gcaaaacttt
    13741 cacttgttca atcgcttttt tctgtttgga caacgattat tttgttcgga agatttcttc
    13801 ataagaaagg gaatcatctt ctgctgtcag ttcatctagt agataacgga tttttttaaa
    13861 gtcgatgaac cgtgcttgcc gaaagacttc ttttccttga tgaaaaatca ggacaactgg
    13921 cgcagtcagc acttcaaatt tactagctac ttctggtgtt tcaaatgcat ccaaatgaag
    13981 tgccggaata tcataatgtg tgagaagttc ctctagtcgt ggacgaacag cgtgacaaac
    14041 actgcaatta ggcatcgaaa gataaatgac ggcaacggat gactgttgca gtatgtcttg
    14101 aacttcagtc aagttagtgg tttgtttcat ttttattctc ccttttatga tagaagtgat
    14161 atgtatgaga ccttaattta atgggaataa cagaaaaagt caattgaagt gctttggtaa
    14221 aaaataagag ttgtgtaaaa atttacagag caacttgttt tttttgaata aaagcgtata
    14281 tttgcaaagc gaatgaacta gaaataaaaa ggaggaaact catgttttct tttattccaa
    14341 attcggtttt gcagatgagt acgattttct tatcgatcgt tattgaagca ttacctttcg
    14401 tattattagg ctgtcttatc tcaggcgcct tacaagtatt tttgacccca gaacgtgtga
    14461 agagatggct tcctgaaaat cgtatcttct ctatttttac tggttcgatc ttaggatttt
    14521 tctttccttc atgtgaatgc ggcatcgtac cgatcgttca tcaatttgtt aaaaaaggcg
    14581 taccagttca cacagcgttt gcttttatgc tgactgctcc gatcatcaat ccaatcgtga
    14641 ttttttccac ttttatcgca tttggcaatt cttggcagat ggcaagctgg cgggtgatag
    14701 gcagcttttt tgttgccctt gtgattggtg tctggcttgc ctattttcaa aaagacgctg
    14761 tattgaaaga aaaaatcacg tctaaagtag cagaaaatca ccatcatcat gtccatgaag
    14821 aagaacaaag tgagaagaat gaaccaaaaa atcagctgat ccatcaaact gggcatgtgt
    14881 tgacacattc aatcgacgaa ttttttgata caggtcgtta tttgatcatt ggcggattaa
    14941 tcgcagctgc gatgcagact tatttaccaa ctagaattat gctaacgctc ggctcaacaa
    15001 agattttagc cattatcatc atgctgttat tggcctttac gatgtctctt tgctcagaag
    15061 cagatgcgtt tataggatct tctttgttaa gtctgttcgg cacagcacct gtagctgcct
    15121 ttttggtctt tggcccaatg gtcgatatca aaaatctact gatgatgaaa cgatatttta
    15181 caggacgctt tattttatcg ctgatcggac tgattgccct tagcgttatt ggattcacat
    15241 tatttattta ggagggaaaa tatgctgcgg tttttgattt tatctgggta tttcgaattg
    15301 atgatgtact tgcaagtttc tggcaaactc gatcaatata tcaatgtcca ttatcgctac
    15361 ttagctattc tttcaatgat tttatctgct cttttggctt tagttcagct gtatttgtgg
    15421 gtcaaaaacg gaaaagcaga agaaaaggca cacgagcacg atcatgacca tgggttgtca
    15481 aaaccttatc aacgtggaat cgcttatgta ctgctagctt tgccagtaat cgtggggacg
    15541 ttgtttccaa cagttagtct cgacactacg atcgtagaag ccaaaggatt taatttccca
    15601 gtaagtaaag aatcagtagg ggatccggag atgcagacac aatacttaaa gccggatacc
    15661 agtatttatt tcaacaaatc agactactta gaccaaatga atgagttgaa agaaaagtat
    15721 ggggataagc aaactatcaa gatcacagat gaaaactatt tagaagttat ggagctaatt
    15781 tataattacc ctagcgaatt cattggcaaa aaaatcagct atgaaggatt tgtttaccaa
    15841 acaccgaacg atagcaatac agatatcttc ttgttccgat tcggcattat ccattgtgtg
    15901 gcagattcag gagtctttgg tttgttgacg catatgccgg aaggagtaac ggttaaaaat
    15961 gatgaatggt ataaaataga aggaaccatc caatcagact attatcaacc attcaagcgt
    16021 gaaatcccat ctgtagttgt tacaaacgct gagaaagtct ctgctccgaa aaatcagtat
    16081 gtgtatcgtt ctttttaaaa gaacaaaata aataaacata gctcagacag ctgacacgca
    16141 aggacgtcgg ttgtctggct ttttttgcat gatgtattca tttgtttgat atttatttta
    16201 gaagcagcat cctgaaagta tttgatatcg gtttcaaagg tgttggaaaa gattgtgaag
    16261 agaattacaa tttattccta acatgtatgt tgaaaagttg tatgaaatga ttttgatagt
    16321 aaaatatcga gtggaggtac tttttatggt aacgcgtata tttattttgg atgatagttt
    16381 ggtttttgaa aaaatgctag tagaaatttt ggaagaatcc agaattttat taagtccttg
    16441 gaaatttgag atcgttcagg gattgaacgt tatacaattt gttgaattca ttaaaaataa
    16501 tgaaatctat tcctcagata tttttttcct agacatcgat ttgaacgcct attatagcgg
    16561 tatcgaacta ggcgaaatgc ttagagaaaa gaatgaaaag tgtaaaattg tttatctaac
    16621 gtctcatccg aataaagcaa taactgttat taatcgaaag attacgccta gtggttattt
    16681 gataaaagaa ttcacttatg aaaaaaataa aaaaagcatc caaagttact tattgaaaga
    16741 aaaagaaaac tatcctgttc aacatgctac gaaaaaaagg tggacaactg tccgttatgg
    16801 taatagcgat cactatatcc attacgatga aatcctttat ctaatgagca tgccaggtta
    16861 taaaaataaa ataggcctag tattgaaaga tgaagagatt ctcgtaaacg gcacaattaa
    16921 aaaatataaa aaatcactcg aatatgagta tttatgtaaa gatttaaaag cttatatcat
    16981 taatactgaa aacattacat ctattagcag agtaaacgaa atagtaacat ttgcaaatgg
    17041 agaacaacta gagatgggaa agagaagtat tgataaacta aagaatttta tgcaagaaaa
    17101 gatagaaaat gaatcatttt aaagaaaaca aagactcata tcttacctgt tagaagagtg
    17161 ttttcctgta gaagagaaca acccaagtag cagcctagtc gtttctatcc ttttttgtta
    17221 tgcactcttt ttgacattta ctccaccaat aacgaagggt acaataaaaa gtaataacaa
    17281 acaaaaggag tacagaaatg attgaattca tcaatgtaag taaaagttac gggaaaaaac
    17341 aagcattaaa agacttgtct ctctcgataa gacaagggga aatttttgga tttttaggac
    17401 ataatggtgc cggaaaatca actacgatta aaagtttagt tagtattatt gaaccatcat
    17461 cgggaacgat tttagtagat ggaatgaaat tgacggaaaa tcgattgagt atcaaacaaa
    17521 aaatcggtta tgtgcctgat tccccagata tttttttaca attgacggct ggagaatatt
    17581 gggacttgat ttcagctgcc tatgaattag acacacaaaa aaaagaaaag cgtttagcag
    17641 aattgacagc gctttttgat atgtatagtc atcaaaatga aacaatcgca agtttttcac
    17701 atggaatgcg tcaaaaaacg attttgatcg gcacgttgct gccagatcca gatatttggg
    17761 tattggatga acctttgcaa ggacttgatc ctcaagcagc atttgatttg aaagaaatga
    17821 tgaaggctca tgccgcaaaa ggaaaaacag tcattttttc tacacatgta ttggataccg
    17881 cacaacagtt atgcgataaa ctggctattt tgaagaaagg tgaattgatt tatcaggggg
    17941 cagttagtga attattgaat ggctcacctg atgaaacgct agaaaagatt tatttgaaaa
    18001 tggctggaag gcaagcgaca ggagatggac cgtatgtata agcgacaatt attagaatta
    18061 attcgagtta atcttttata tgttaatcca cagctcacga ataaagcaag agagaaagga
    18121 aaaaagggaa agcgattaac tatctatctt ttgtctcaaa atcttttttc agggctgatt
    18181 tttttactga tttacggatt tgctatgttt gctatgaact ttgttaaatt gccaggtttc
    18241 tttacttatt acgtggcact ttttggtatg ttaggtttaa ctcaaagtat atcagtcatt
    18301 tataatatct tttttgaagg aaatgacctg caagtatttt tgcctctgcc ttttggacaa
    18361 gaacaggttt tttcagcaaa gattttggta gttgctttga ccatcattcc ttttgttttt
    18421 ccgcttctag tcatgtttat tttaaccgga tggcgagctg atctagggat tttgcttact
    18481 atcattatct caatcctttt gttttctcta tttttgatta ttcttttttg tctggctagt
    18541 ttgattgttt ttggtttggc tcgaacagca gtgtttaaaa aacatagaaa aacagtttct
    18601 agtatcttgc ttggcagctc gatgctgatc gcatttgctg gaatcatgtg gatgaatcat
    18661 caaacgggga ctattgaaaa tgagttaaca gatcggcatc cgatttcgat attattgcca
    18721 ttctatgatg ctgccagtca gccactttct caacaaggtt tattgggttg gggaatacta
    18781 gctatcacgg ctctgggatt tttccttgct gtaaaacagc tgatagtacc aaagcttaca
    18841 gagcaactga ctgattcaaa cagtgagttc aaaataaaaa gaacccataa aaagaaccaa
    18901 aatgtgcatc agcttttatt tagttacaat gctcaattag taaaagaacc taatttgatt
    18961 atgcaagttc tttcttcttc cttgttaact ccaatcattt ttattcttgc ttttgcccta
    19021 ggaggcgaaa taaaattaac tgatttagat aataagttaa tcggtgttgt tttcctggta
    19081 gggatcgcat tggctttctt aacagttaat cagacttctt ttatcagcaa tcttatttct
    19141 ttggatcaag aaaattttct gtttatccgt tctttgccaa tctctatgaa ccaatattta
    19201 aaagaaaagt tcagattcgg atggatattg cagagttttt taactggtgg gatcgcatta
    19261 ttaagcggac tgtcttttca gctgcctttg ttttttatcc ctagcttgct atttggttct
    19321 ttattcggct gctacttatt gtcactacgt tatttcgcta gagattatcg gttgctgctg
    19381 ctgaactgga ccaatattaa ccaattattt aacagaggtt caggaagttt gggtttagtc
    19441 gcaactatga tgggttcatt gatcatcagt attattctta tcgtcttata cggaatgttg
    19501 gctttgtttt atccattttg gttagtgaat attcctgtga tcggtttagt tctaatactt
    19561 tccgctctat ggatcgttta ttatcaacga acgttttgga aaaaatttga ataaagaaga
    19621 ggggccgtga caaaaatcat gtcgcagccc cttttttaca gatagagggt gttcaaaagt
    19681 cagtttctca gtattaagtc aattttatgt aaaaatgtga gaagctattt ttacaaaact
    19741 ttttttaata ctcataatta cttcttattg ctcctgcggt tacttgtcgc aatgtttgtg
    19801 agatattttg tcatcgctca aggctgaacg tctctttcac aaccttttct ttgttacact
    19861 tcgtttattt aaaaagccgt ttttttcgtt tcatcatgat tgcgtcacgc tttactttga
    19921 aattacggat gttatggtta aagacttctg ataatacaag acctaaagcg attgctccag
    19981 caatcataat tgcttggacc gcataatgag ccgcttctcg ataggattca gtaacgagat
    20041 ttcgaaccgc ttgataagct aaaccaccag ggactagagg aaccatcccg ggaatattga
    20101 aaatcgttac gggcattttc aaacgtttag agaaaagatc gctgatgaat gctactccta
    20161 aagaaccaag caatgaacca aaagcagcag atccgcctat ttgaacacag atccagtaga
    20221 tcatccagcc aaaagcacca gaaagaccac aacaaatcaa tgaacgccta ggtacatttg
    20281 taataacggc aaatgccgca gtcgctaaaa aactaaaaga aaactgaata aatatcgtcc
    20341 ccatacttgt gttcctttcc taataaaaaa gctggaaaat aaaagcaatc gcaaaaccga
    20401 tcatcgtagc agtcatcatc gcttctgttc cgcgagaaac tcctgagaca taatgtccag
    20461 ctagcaaatc acggatagcg ttcgtaattt gaactccagg aactaaaggc atcacacatc
    20521 cgataatgat tagatcctga ttgcttccta agccaaaacg tacgctgtag atagcggcta
    20581 tgccaatcaa taaagaagat aaaaattccg ataaaaattt gattctaaaa aatttcaaac
    20641 tatatagata agctgcgtat cccaatcctc caatgactaa agtcacaggt aaatcggtca
    20701 tctttccgcc aaataacacc ataattgttc cactgatcac tgccgcgcta acgatttgca
    20761 accagacagg aaagaaagaa cggtctcggt cgattgcttg cagttcgcag taaagttctt
    20821 ctaatgttag ctctgaagca acatatttac gtgaaagatc atttactttt actacttttt
    20881 ctaaattaat cgaacggttg gtaatttgtt ccattcggat ggtcgaggta cgatcaagac
    20941 caataaagag accggtttga gtgacatagc tgaccaaacg gaaatttcca gaagctaaag
    21001 caattcgact catcgtatct tctacccgat acatctccgc attgctttcc atcatgattt
    21061 tcccagcgag taaacaagtg tccagcaaca gatctaattc ttttcccgtc atagcatctt
    21121 ctccaataat tgattgatta gttttatttt actacaaaac ataccagaag cgtaaagaat
    21181 aaaaaaactt gaggaaggaa taaaagaggc gtatactgtt tttgtattgc gggtgtagtt
    21241 tagtggtaaa atcccagctt cccaagctga tgtcgcgggt tcgattcccg tcacccgctt
    21301 aaaataggtt gtgaaaaagc tgctctgcaa caagtaataa gaacagccaa gcaaaaacaa
    21361 cttcttatgt ttttaacttg gatggactta ttaccgcaga tgcaagcttt tgcgcaccgt
    21421 ttaccaagcg gttgtgaaat cagtgtattg tcttgagtat gaagaaaatt tttgaataat
    21481 aataaatacc ttacagatag agtggtgact gattagagac gaggaatccg tctctttttt
    21541 gtatttttaa gccatcttaa aacaggtttt attttttgct gtttctattt ctttctagta
    21601 tttatttata ggggaacata tgttaagatt tagggagctg ttagtaaagg agtaaagaaa
    21661 tgagcaaaaa aacaaccaag gcgatcgtgg ggatcgtcgc tgctgtgatt gcaggggcat
    21721 tcggtgtttc caccacacaa aaagattcag atatctttca gcagattaca actatttttg
    21781 attccacacc acaagcttct caagtagcgg atgtgccaga atatgatgga aaacatcaag
    21841 aaatagaaat caatgggaat cagccggaat ttacaaaaga ggatcttagc ctttccaaag
    21901 gaacatggga aagctattca aatattgata aattgaacag agtaggacaa gccaatgcta
    21961 tgcttggaaa agatttgttc cctactgaaa agagagagcc tttgtatatt gacccgacag
    22021 gttggaaaca aaagaaatta agtgacgggc aatggttata taatcgcagc catttaattg
    22081 gtttccagct gactggtcaa aataacaaca ttaaaaactt gatgacaggg acacgttcgt
    22141 tgaatgctcc atacatgctt gctcacgaaa atgatattgc ggcatatatc aaagaaacaa
    22201 atcatcatgt acgctaccgt gtcactcctc attttgaggg gaatgaactg gtagccaggg
    22261 gagtgcaatt ggaagcagaa agcattgaag acaaaaaaat tgaattcaat gtctttatct
    22321 acaatgttca agatggctac acgatcaatt atgagacagg acaagcaaca aaaacaaaaa
    22381 attagtagca gctattcgaa ttttttatga atattatgaa aaaattgtgt aaaaacatac
    22441 attcaaagaa atatagcgta tactttatga aaagagtact tttgataaag ctacagaaaa
    22501 aaggaaaaaa acatacaaat agtgtttctt aatttattcg gaagtttgga atatttgttt
    22561 gtagttttcc ctttcttcga gtaaaataag gttgtagaag gtaagctgga attaccttgc
    22621 gggaaagagg tgtagaatca tgggttttac agatgaaacc gtacgttttg gttttgacga
    22681 tagtcgcaaa aaagaagtga gcgaaacatt agcgattgtt tatcgggctt tggaagaaaa
    22741 aggctacaat ccaatcaatc agattgtcgg ctacttgctt tctggagacc cggcctacat
    22801 tcctcgttat cgtgatgctc gaaatttgat tcgccgtcac gaacgcgatg agatcatgga
    22861 agtaattgtc aaggactacc tctcaaatca tggagtgaat ttatgagagt aatgggcttg
    22921 gatgtcggtt ctaagacagt aggtgttgct atcagtgacc cctttggctg gacagctcaa
    22981 ggagtagaga tcattcggat caatgaagca gaagaagagt ttggctttga tcggttgaag
    23041 gaattagtag cacaatatga agtagaccgg tttgtagttg gattacctaa aaatatgaac
    23101 aattccatcg gaccaagggc agaagcttcc atgtcatacg gagaaaagat caaagaacta
    23161 ttctccttgc cagttgatta tcaagatgaa cgattaacca ctgttcaggc agaacgtatg
    23221 ctagtagagc aggcaaatac ttcaagagcc aaacgaaaaa aagtcatcga taaattagca
    23281 gctgtgatga tcttacaaaa ttatttagac ggctcaggta agcaattatt ttaataataa
    23341 aagaaaagag gtaatcttat gacagaacat aaccattccc acgaccatga aggacacgaa
    23401 cacatcacat tggtggatga ccaaggaaat gaaacattat atgaaatcct actaacagtt
    23461 gacggacaag aagaatttgg acgtaactat gttcttttat atcctgctgg tgtttcagaa
    23521 gatgaagatg tagaattaca agcttatgca tacatcgaaa acgaagatgg aacagaaggc
    23581 gagttagaac aaatcgaaac agacgcagaa tgggacatga ttgaagaagt attcaataca
    23641 ttcatggcgg aagaagaaga atagactata aagctaggag aaaccttgat atgatagggt
    23701 ttctcctttt tttatttttt actgtatgcc atattgtatg ccatctagtc aatatttaag
    23761 tgttgtacta atttttcaac agtcttttct ttcgtttctt ttgccaagtg accgtaacaa
    23821 tcgatcgtct gactaatttt tgtatgccgt aatctagttt gaacttctac catagtagct
    23881 ccagcgtcca agagcattga agcatgagtg tgtcttgcta catgcacacc gagaaatgga
    23941 accatagctt tttcacaaat agtaatcaat cttgcataaa tagcgtggtg tgtgattagt
    24001 tctttataga taccacaaaa aatcatagtc ggatcagtta caccaagtgt cagcatatat
    24061 ttccgctgat taatctgcca tttttttaat atagaaattg ttctttcatc tagtggaaga
    24121 accccagcac tacaatcagt tttaggatca ctgattttat agccattttt ggattggctt
    24181 agtgttttat tgatggtgac cgtattttct tgaaaatcaa tgtctgacca attcaatgca
    24241 agggcttcac tgatcctaac cccgctataa aagagaaagc gtagtaacgt tttatcaaag
    24301 tcgtttcgat aagtttcagg tttgctgttt aggtaatcaa acaaggtgat cagttgttct
    24361 ttactgtaaa ctttgacctt ttctttatgc ttagtggtgg ttcttttagg cattaatacg
    24421 ttgtctagtg gattagtctc aataatccca agattgatag cgtagctaaa aatacgctta
    24481 gaagttgaat gaagtacctt gtattggtca gtagtttcag cccagctatt tacaatttgt
    24541 tgggcttttt ttaccgtgac ttcattcaca cgaagatttc catattttgg aagaatccac
    24601 tttctaaact tgatttcagt tttcatgaag gtggcttctc ggacagtggt tttatatgtt
    24661 tctagccaca agtcataaac ttctttaaac gtttcgttgt tcgctttttt aataccgttt
    24721 ttatcgtagt ccacttgcaa gcgtgacaag gcaagctggg cttctttttt tgttttgtaa
    24781 cctcgtctac gaaccttatc aggtttgcca gtcattttgt tcattccaag ataggcttca
    24841 tagagccaaa atttaccttt tttagtttca tattgcttga atgttgccat aatagttttc
    24901 cctccatagt gtagcaagcc gtgggggcgg ttggaggagg cagtttatta ttattttgaa
    24961 tagtgtttat ttagctcagt aacaagagct gtgaactctt ttagaagatc attttcataa
    25021 aattcttgat actctttttt ctcatctgta tttttttctg aatcattaat taatgagtca
    25081 tatcgtaaaa gcgtatttag aagagctgcg agataagcaa ttggggtact cccgtcagga
    25141 aattcaattc gagatcctgt tttaagatat tttattgcgt ttgttaaata aatcttctgt
    25201 gagacagtca ggttatcttc ttcgatgtta ttgatagcat tagaaagatt agaatcaatt
    25261 atagagttat ttttttcaat agtttttttc aattgctttt caagcacatt tcgcttaatt
    25321 tcgggcatca tatgcatatc tcttaatgta taagatccgt ttaatagata ggtagtggat
    25381 acatctaata cctttgaaag ttgtttaagc ctggtgttat tgggaagatt atagccattt
    25441 tcccaattac taacggcacc tttactggcc ggtggggata atttacctcc aagttgttcc
    25501 attgagagtc ccttttcaat tctagccagt ctaattcttt gtcctgtctc cactgcttcg
    25561 gggtattttt ttctagccac attttcacca ccattcatat attttctcta ctatatcacg
    25621 atttaaatat gtgtgaaaca taaaagttca aaaaagtatg ttaaaattct tgcaaatgaa
    25681 aaggtatgat ggtatattga gtttataaga ggtatagaaa tgtattcaag agcctcttgc
    25741 atttttttaa aatctttgta tagaaatgta tgctaacgag gggggataaa tatggaatta
    25801 aaaattgaca aactcacgct tgatgatcca gcttttaaaa ttattgacga tcgagttgtt
    25861 caacaagtag agcaaactca aggaacgtta ctttcattat tagaaaaaaa gtaccagttt
    25921 ccaaaatata tgaacaaggc acaagctgct aagtatatga acgtttcata caatactatg
    25981 atgcagaaat atgttcctaa cggtttaaag ttgattatag ttgatggagt cgtaagaatt
    26041 tcacaagatg aatgtgatcg ctttatggaa gaataccaaa aataaacgca agccgtgggg
    26101 gcaaagggat taataggagg aaataataat ggaatcgaca caaaataaag caatcgaaaa
    26161 agtattatca acaattctta cagaagacac agcggcagaa ttagcaaact tggaaggcaa
    26221 agcgctagaa gaaacgtttg aatgtttgta cgaacaaatg gactatcaaa aattgctacc
    26281 acaagaacca acggtaagcg gagtattgcg tggcttgaac gatttagtac aagcagagtt
    26341 taaagaacga ctttctattg aagagtatca agaaattttg tatcagcaag ttgagttgtt
    26401 agcgagcttg ctagggcttg aactggagga cgttgaatga aaactgaaat ttggaacgga
    26461 cacattataa gatttgtcga tattaatgat gaatggtggg cagtggcgaa agatgttgcg
    26521 gaagcgttag gtcttaaaca ggttactaga gccattcact ctttgcctaa agatggggtt
    26581 actactagta aggtcatcga ttcattgggc agaacacaag atgtaaacat catcaacgaa
    26641 aaaaaatatt taccgcatgg cattcaaaag ccgtaaaaaa gaagccgagg catttcaaga
    26701 ctggattttc gacatcatca aagaactacg ccagtccaca ggacttgaag gctttcaagt
    26761 attccgcatg ctagacaaag aacaccaaaa agaagcaatg actaaattaa gccacgctat
    26821 tactgaacca aaaccagtag actacattaa agcgaatgtg atagccaata aagctgtatc
    26881 aactatttat ggtcattcca aaatggtaaa aaagaaagat atgactcctg aaatgttggt
    26941 tgatcgtgaa cctattttag atgagacagt agaattaatg actgttaaag aaaaatatgg
    27001 gttgcagttt tcagtgagtg agaaaattta taatcgttct gctgaattgc agacaactta
    27061 ggagcattaa catgatcgag aacaaagaaa aagccttatc cataacagtc ttggcggaca
    27121 ggataaaggc aacttaattg ggataaaacc cgatattctt tgttccgatt ataccatatt
    27181 aaaaaatcgg agggaaccat tatgaaacga aacttaatta taggaagcat tttaatcggc
    27241 ggaattttcg gcttattacc attaattgca aaaatagcta tctttgcagg attagcgtta
    27301 tatctcttca ctgcttatga cgagtacgac tatcaattaa gaactggagg gcataagtag
    27361 atgtacaaaa caaaactatt aaaccaactc gatagccttg aattagagga aattaatcaa
    27421 gggatcgctg aactagaaaa taatatcggg aaaacctact ttggaaattc attcaatgaa
    27481 aaactaacag ttttgtatgt actcaaaaaa catgcagaac acaaaataat ttgtcgagaa
    27541 atcaacgagc taaaaaatca gattttaacc gcatggttaa acattactga tatgcaagaa
    27601 gcaagggtga aaacatttaa tacatgggta aagtatcaga atcaattaaa aggtgctgaa
    27661 tttgttcggg atgggttgaa atacgaactt gagcagttga agctcatgga ggtgtctgaa
    27721 tgatttatac cagtattttg aaaaaggagg agaggtttat tggcaaataa ttatcaagca
    27781 gcaacggaat tacaaaagtc gggatggtca atttatccct tatctcctgg aactaaaatt
    27841 ccaataaaag gctctagtgg ttggaatgat gcaactaacg attctaatca aattaatgaa
    27901 tggtggacgg aagatgcaac tagaaatatt gggttaatgc ttgaagatca tcaaatggtg
    27961 gtagttgacc tagatcaaca ttctgaaaca cgaaacggcg ttgagaatta taaaaaactt
    28021 gcttcaatgt atgatccatt tccttctact tatacggaaa tcacaccagg taaaggaatc
    28081 catttcttct ttaaaattcc tgatggtgta attatccaac aagaaacagg tgcatttgct
    28141 gatatcttcg ggcgtgatga taaaggtaaa agtttaagcg ggattgatat tatcactaaa
    28201 ggagttccaa tagcgccaac tatgattatt tcaagaagtg ttgggagagc atatgaacct
    28261 attgaaaacg tatctctaaa taatattgca ccagctcccg attggctagt gaacctactg
    28321 ctaaaaaaac cgcaagaaat aaagactcaa taccgacccg tagcaaaaac agggacagca
    28381 aaaaaattag atatgattgt tcaaggcgct ggcgaaggta gtagaaacga tcatttaact
    28441 aagttatgcg gttggttact atggcacggt gtagataatc aaactttagc agaattgatt
    28501 tatacagcaa atgcgtacaa tgaaccaccg ctaaaagata aagaagtgaa tcaaattatt
    28561 cgatcgatga ttaaaaaaga tttgaggggg aaagctcatg gctcataagc ataaattaga
    28621 tagtatttta gactttcccg aagccagcga gcgtgaagac aatatcattg aattgaaaac
    28681 ctggatgtca cgattaagat gcaataaaga cgatcagatt aaaagtaaca gtgttgtgaa
    28741 tgcagaattg attttaacca atgatagcaa tttagctgga acaattgctt ataacgaatt
    28801 tagcggatac attcacctat taaaagattc tccttggatc aatcgtaccg ctggagaatg
    28861 ggaagacagt tttgaggatg ccttaacggc atatattgaa gaaaactaca atgtagtttt
    28921 tgatgacaac aaaatacaca aagcagtagt aaatgtggct agaaagaatg ttttcaaccc
    28981 agtaaaagaa cgtatcgaaa aagttaaatg ggatcaacag cctagactgg aaacactgtt
    29041 tattgacctt ttaggggtag acgacaacct ttacacacga gaagtaacaa aacgttggat
    29101 agtcggatca gtcgcacgta tctataaacc aggaataaag tttgagatcg ttccagtttt
    29161 ggatggaccg caagggattg gtaagagtac cgttccagca ttactttaca cggatgattt
    29221 tttcaccgat tcattagatt cgctaggtga aaagaaagat gattatatgc agcttcaagg
    29281 caatgtaatt attgaacttg gagaattatc ttcaatgaac aaaactaaaa ttgaacagat
    29341 aaaaaacttc atcagtgcca agatcgataa aattagacct ccttatggtc ggaatgtcat
    29401 agcttgggca aggcaatgtg tatttattgg aacgtcgaat gatgggcagt atttgaaaga
    29461 tgatacagga aaccgccgtt tttatccttt gccttgtaag aataagccaa agatgaatcc
    29521 gtttaaaaca aaggatgaat attttcttca agtgttagct gaagcaaagg tgctttttga
    29581 taaaggacaa cgaatctatt ttagtcccga tgaagataaa gaagtcttag aaattgcaaa
    29641 agactatcaa gaggatgcca agatggagaa tccaattaaa gaagcaatca ctaaatattt
    29701 ggaaatggaa atcccgtata aatgggaaga agctccagcg tgggcgagac gttcttatta
    29761 tcagaactat cctgacagta aatgtaatga gaaaaattta cagcatttct ataatggagc
    29821 tactatgcaa caagagtttt atttagttga tagcgtactc accgctgata ttttggaagc
    29881 tgtttttgat aaacaagcaa aggatttatt gaatggtcga tcagatgcag aaactaaaaa
    29941 gattgccttg attattagca gtattcctgg ttgggaacgg aagcaactgc caaaaagaaa
    30001 taaacgacga ggattttaca ataagtcaaa tgcctatgaa aataaaaatc gagctggaaa
    30061 gtacaaaaag taacgggtag taacgggtca gtaacaccta gcccgttact agtttaccct
    30121 tactgcccca agggatacag caatttggta acggggtaac accttatttc cttaaaactt
    30181 ttgaaatagt tagctaatat gctggggaat aatgttactg gattttaccc gttacctgtt
    30241 accaaaacgc tagaaccctt gtccccgttg ggattgagtg gtaacgggta cctgttacgt
    30301 aggtgttacc acgtgttacg aatcaaggag gaaagtaaat gctcaatatt atttcaacaa
    30361 acaaagcacc gaactttcaa tatacagatg aaatggatcg gtttctaatg aacacgctgg
    30421 catttagtgt gggattggta acagaagatt attccacatt tgatccagaa gtattaaaga
    30481 taatggaaga agaacctgat tggctgcagg agtcagtagc gtggtgtcaa tcgctagtag
    30541 tgggatcatt ggtagatagt ggcaactatg atgatacagg tgagctaatg gatgaattta
    30601 attgtctgct taacctatat gatcgagcga gacaacgaga gcttacatcg aatgaggata
    30661 atttgttttt aaacatccat gataagttct tggctttgct actaacagat gatgaattga
    30721 taactaattt attggaggta gaataattat gcatatggaa agtattagat acttcgatgg
    30781 tgagaaacac attgagtatt caaacactca agaagatgta gactttatta gttttgagga
    30841 agataaaatc actaaagtta attttaaaga cggaacgtgc ttgaagatag tttcgcctta
    30901 tattgaatac aaatcaaaat gggaacaaga tgattctgac gaacgagatc gctttggttg
    30961 gtaatctatg gcagttaaaa aacaatgtaa tcatgctgga tgtaaaacat tgattgatta
    31021 taggcagaag tattgtggga agcataaagc aaagcaaacg gcaattaagc gtgaagaaag
    31081 aaagcaaagc gaaggtaaat attttcaatt ctatcaaagt agaacatggc gaaaggcttc
    31141 atacttgtat cggttaaatc atccagtatg tgaggattgc ctagaagaag gcttgataag
    31201 gaaagctgat gttgtagatc acaaaattga actaaaagat gattggaata gacggttaga
    31261 tgaaactaat tttcgttctt tatgccatgc acaccataat tccaagacag ccaatgaaag
    31321 acaaagacga gaaaagagta ccctttgagt taagggggca tagtgtgaaa cttactgaca
    31381 atcgatgcct actcattttg gtacaaataa ccgttgtgaa aaggcataag ggtaattaca
    31441 aataacgatt acacttgtaa tcacaagtga aaaagtatat aatgaaagta ggagatttat
    31501 tgaaaaatac aattgttatt actgataaaa cagggaaaca gcgcaccatt gagttacctc
    31561 attttggtca agtgaccatt caaatgcaga acggaaaaat catttatata gataaattag
    31621 ataaagaaaa attctgatcg aaaaacgaag gaatgtgagc ttaattgctt acgttccttt
    31681 ttcttttgtc tgaaaggagg acaacatggg acaccctaaa atattacacg atacaaaagg
    31741 caatatatca agtgaagaaa aagcaatccg tgtggacgct agagaagaat tgttcaagca
    31801 gcaaccatta ataaatatca cgccccctga ctggatggca gcgagcgcta gaagtgaatg
    31861 gaatcgtatc gtaccaacat taaaaaaaga ttatccattg agtgaagcag attatggttc
    31921 attggtagcc tattgtttag cctttgctcg aatgaaaaca gccgaagccg agataagaaa
    31981 atcaggtacg tttatcacat gcgaaaacgg agtaaagaaa gccaatccag cggttcgggt
    32041 tcaatcacaa gctatgagtg atttgaaaaa acaagccacc tcactaggta tgaccttaga
    32101 atcacgatca aaactagctt tgaacaaggt taaaaatgat gaacccgaag acccgttcaa
    32161 agagttgatg aattcatgaa tgattacatt gagaaagtcc tatcaggtaa gctgattgca
    32221 cccaagaaaa ttatccaagc gtgtgagcgt catataagcg atttggagcg ttctaagtca
    32281 gatagcttcc cttatgtgtt cgatgaagaa caagccacca aagcgattaa attcattgag
    32341 ttgctaccat ctacggacgg taaagcaatc aagatgttag gatttcaaaa attcattcta
    32401 ggcagtcttt atggctggcg tactaaagaa gggaattaca ggcgattcaa tcgagcgttt
    32461 accagtatga gccgtaagaa tgggaaaacg tatatcgcaa gtggcatggc tgccaatgcg
    32521 ttgattatgg aacaagaacc agcagaagca aggcaagtat tgtttgtaag taacgctttg
    32581 aaacaagcta aattgggcta tgatatgctg tctaattcac ttagaaacgt ggtcaagtct
    32641 agtaagtttt taagaccaca actgaaaatt atgaactcta agattcagca cttgccctct
    32701 aattcgttcg ctatggcact ggctagtgaa accagcacgc tagatgggtt tgcaccaaca
    32761 accgcaattc ttgacgagtg gcacgaagca aaaactcgta aaacgtacaa cgtcattaag
    32821 tcaggaatga cccaacaaaa gaacggctta ttgtgtgtta ttagtaccgc tgggcttgat
    32881 ttaaacgttc ctatgtacga ggaatactta ttgttagatc gtgtgctaaa aggcgaagaa
    32941 caagccgaca ggtactttat agcgatatgg gaattggacg atcccgaaga aattcacgat
    33001 caagagaaat ggatcaaagc caatccgatt tttgaaagtg aagaaatcaa aaaagtaatg
    33061 attccaacca ttcaagatga tgtgaacctt gctttgaaac aaaataacct taattctgta
    33121 ttggtgaaaa acttcaattt atggagacaa gcgagtgagg acagctatat gattgctgaa
    33181 gactggcagg caaccgaagt agaaccacaa gatattacag gcaagcccgt ttatatcgga
    33241 gtggatttat ctaaaacaga tgatttaact agcgtttcat ggatcgtacc gctagataac
    33301 ggcgaacttt attgtgattc acatagcttt gtagccacca aatatgggct tcaagacaaa
    33361 gaaaagcgcg acggtttgcc ttatcgagaa ctagaaaaag ctggtgagtg ttccattact
    33421 caattagaaa gtggaatcgt ggactatgac caagtatttc agtttattca agatttgatt
    33481 caagaaaatg atttggaatg tatggggatt tgttatgacc cgtataacgc taattcgctt
    33541 atcagtaaag ctgaaaaagc caactaccca atgttagaag taagacaagg aacgattact
    33601 ctaaacgttc cgacccgaac ttttagagaa caagtttatg aaggcaacgt tattcacaat
    33661 aaaaatacga ttctcaccca tgcagtgaac aacgctattt taaaaacgga taacaacggc
    33721 attcagatta ataaatcaaa gaacagtaac aaaattgatc caatagctgc attaatcaat
    33781 gcctatgtgt ttgcaatgga ttacttcacc acaacggaag gagcgaaagc agacaatgaa
    33841 ttttatacaa gtgaagaatt ttctttctaa ttacattcat accgttcttt tacttcttgg
    33901 attggtgtgt gtgttggttg ctatcacctt actaacaaat gtctactatg gcttgttagc
    33961 gctgggcata gtgcttattg gggtagcggt catgctaaat acagaacaga agggaggtta
    34021 aaagatggca ttttttaaag cgagacaaaa tacaacggga gatcctttcc tacaacatgt
    34081 ggtatcaatc caatcggatg attacaccac cagctttaca agcgttcgtg cattaagaaa
    34141 tagtgatgtg tttgcagccg ttcgaattat tgctagtgat attgcttcaa gtccgattca
    34201 attggtcaaa aacaatatgc cacaagctga taatgaactg gtgaagttac taaacgagaa
    34261 acctaattca gaaatggatg gttggcattt aaagtttgct ttggcggtca atatgttgct
    34321 aaacggtaat agctttgcag agattaaacg caacggtgaa aaggtagaag aaattcactt
    34381 attacctaac tcaagtgtaa cggttactca attagataat ggtacgttgt cttatcagat
    34441 tggtgataaa aaaagacgtg tgaagtctag cgatattttg cacttcaaat atttcaccca
    34501 agatggttta acaggattac caccacttta tgcgttacgt gatgaactga aaattcaaca
    34561 agctggcaac cgcacattgc acaatttctt tactcgtggt gtcagtgggt caggtatttt
    34621 gaaggttcat aagtcagatt tagacggatc agcaaagaac gcaattcgtg aaaagtttga
    34681 agaagccaac ggttcaagta gtggagataa tgctcttaga acgatcattc ttgatgaaac
    34741 aatggactac aaaacattag aaataaacac cgatgttttg aagcttatca attccaatga
    34801 ttggaacaca aaacaaattg ccaaggcgtt tggtgtaccg attgagcgtt taggcgttga
    34861 aaatgaacat tctagcaccg ttcaaagtaa tctccagtat atccaaagta cgctgattca
    34921 ttactttaat gtgtttgtga gtgaattaga tacgaaactt gaaaccaata tccgctttaa
    34981 ttccgatcag ttactagaga ctgatcctga aacaaaagta aaaaatgtat tggatcaggt
    35041 caaagggtca cttctcacga ttaatgaggg gcggtcgaaa atggggctac cccccatgga
    35101 tggtggcgat cgtttactag caagtttgaa ctttacgtat ttagatacgt tggagaaata
    35161 tcaattaaaa gaacaggaag gagttacacc agttgaataa tgaagaagaa aaggaaaaac
    35221 ggctgacaga agaagctgag ctaacagccg attctcccaa agtagagaaa gaaaatgaaa
    35281 aacaaccaac agacggcaaa actatttcag gctatgcgtt gaaattcggg caaccgtcaa
    35341 aagatttagg cggctttgta gaagtgatta cacccgaagc attaaaagag gtggatttat
    35401 caaatgtgtt cttattatat ggacacgatt attcaaagcc actagctagt gtcaaagcag
    35461 gtacgttgaa attgaatgtc gatgatacag gattacattt tgaagcagag ctaaccgaca
    35521 cgacctattc aaatgatgtg tatgagaata tctcaaaagg ggttgttgat tctatgtcct
    35581 ttgggttcgt gcttggatta gattcattcg acaaaaaaga agatggcaca attgaacgat
    35641 caatagataa aatcaaagca cttaatgaaa ttagtgtcgt gaccgttccc gcttatgatt
    35701 catcaaatgt ccaagtaaat aagcgttctt acgaatcgtt tatgagtaac aaccaagcaa
    35761 aacaaacaaa caatagctta gaatccactt ctaaagcaca aaaggagagt aataacatgg
    35821 aaaaaacttt aatcgataac gaaaaaacag aattgcgtgg gtatgaagaa tatatccgtt
    35881 cacaaggcga agtgcgtgat ggagtcacta ctgtaaatgc agcggcagtt gttcccgaag
    35941 aagtaatcgg tgaagtcttt gatttgaaac gttcaaatta taacttagct caatatgcaa
    36001 cagtaaaaac agtatcaaat ggacaaggta aatatccagt agcaactaac caacaagcag
    36061 tgttagcaac aaaagctgaa cttgctgaaa ttggtgatat tgacgctgaa atgtttactt
    36121 cagttgatta taaagtagaa actcgtgctg gtaagattgc cttatcaaat gaggttgtgg
    36181 aagattcagc agtgaatatt gtacaagagg tcaaagatca gttagcgaaa ttggtagaaa
    36241 acactgacaa taagcatatc atggatttat taaaaacatt cactaagaaa acggctgcta
    36301 cgttggacga tttgaaacaa ctatacaatg tggcattaga cccagcatta aataaaatgg
    36361 taattctaaa ccaaagcgga tattaccacc tagatacatt gaaagattca gatggacgtt
    36421 acattttaca acccgatgtg acagcaccta gtggtaaatc attattcggt atgccagtag
    36481 tattgattgc agatacattg tttgccaatc ctaaagctgg aacattccct atgattatgg
    36541 gggatattgc acaatctatc tttgttgctc gtagaaatca agtaacgact caatgggaaa
    36601 aattcgatta ctactcacaa ggacttgcag tgatcgttcg caacgattat aagaaaattg
    36661 atcaaaatgc ttcagtgtat attgagttta cgccagttgt aacaccaaaa gtatagaaaa
    36721 atattgggcg gcggtttatc ccactgcctt tttttcatta ggagagataa aatggtaatt
    36781 ttggatagca taaaaaaaag tatgcggatc gatcacacta ttgatgatga ctttattcaa
    36841 caattgattg atacagcagg cgaatatata aaaagtgcta ttgatagtag cgcaacggat
    36901 aaagatatgg ataattatca gcaatttgat ttggcggtgt cattacttac tcaacattgg
    36961 tatttgaacc gtcaagaagc cagcagcgaa cggataccag taacggtaca agcattagta
    37021 caacaaatga gaggtgctta ttatgccaat cattaagaat gtaaatgaat tgactgagag
    37081 agtcaagttt aaaaagacaa aacgggtaaa agatgaagat gggcagatgg tggatagtga
    37141 agaaactgta tttgaatgtt gggctaatgt gcgttcacaa atgctaaaag acgtgcttgc
    37201 tagtgtaggt accattcttg aaggaacgtt gacgtttatt attcgatacg atcaagatta
    37261 tgaactaaca aacgatatga aagtagcttg gaaaaacaag agttataaaa ttatttcaat
    37321 caatgaagga acggcgttta aagattatac aacgatcata gctaaacaga tttcttagaa
    37381 tgattacact tgtaaacgtt gtgggtattt agtataatta aggtagtaaa tgaagggtta
    37441 gctgcctgaa ttttgcaaga cttagctagt cgaaatctat tgttggactg aaaattgtag
    37501 gtgtggttgc aaacatgctg gactaagaaa gatgatagat gatgaacgtt caattttaat
    37561 acaggcatgc taggactgat tggatggttt tggcaagctg attgtgtaga aatcaaagaa
    37621 cacgtctgtt gtggacgtgt tttttttgtt ataattcaaa agaggtgatt taatggacaa
    37681 agcagagttt acaaattttt attggaagta ttatctacat ttagaaaatg agtttattaa
    37741 taccacagat tttgtgatgc tcgatgagaa taatttcaaa acattctcta ttgaatatca
    37801 aaaactacta ctagcgattg gttcggagtg tgagattatt ttcaaagaac tttgcggatt
    37861 tgatagcagt agcaataaaa gcataactga ttttaaagct gttattaaat ctagcgaatt
    37921 aatgtgtttg gataatggtg ttagggttat gaattcttta acattgactt cactaaaacc
    37981 ttttggagat gggtggcctg aagatacacc agattggtgg aaaatatata acagagtaaa
    38041 acatggtcgt tcttcaaatt ataaggaagc aaatttggaa aatgtgttgt atgctttagc
    38101 aagtctatat ttattagagg aatatttgca taagaaagta atactagaag gtgaagtaga
    38161 ctttataagt ccagagtcaa atctatttac gttatcttgg gaaagaaaac attcgtcgat
    38221 gcaaaaagtg acgtttgaaa aagtggattc aaattatgta gctccactca ttgattttaa
    38281 agaacaagaa gaaatttaaa aacccccacg gcttattgaa gtttttgtat gccatattgt
    38341 atgccatttg gtttaaatcg cttgaaaaat agtgatagat aactctaatt catttagtca
    38401 taaacgctgt tttgataacc tttatagttc tttgatatgg gtttctcatt catggcggaa
    38461 gaagaagaat agaacaaaag aatgaattaa taagatataa aaaaagggat tgtgacaaac
    38521 gtcttgtcac aatccctttt tccaaataaa cgatgttcaa tcagctgccc ctcggcattc
    38581 gctcgaaaaa cggcgaaata tgaagaacta ttttgccatt tgtttctcta ataatcaggt
    38641 caaaacgttg acttcacagt ctcttcctta aacggtgttc tcctgccaac tcctcggtca
    38701 caagtcacaa tattcaacaa acatgagaag ctgttttttg aaattgttcc ttgtgactcg
    38761 gagctgaacg gcaggctcac gacctctttc ttaaacggtg ttcaaaagct tgcatctgcg
    38821 gtaataagtt caaccaaacg aaaattatga ggagctattt ttgtttggct gttcttatta
    38881 cttgatgcag gacagctttt tcacaacctc ttttcgttta gcactaaatt tccttaaaaa
    38941 gtagaaaagc tttcgaagcc tcgtttgttt cttttactgt ataataaaag aaaagagtaa
    39001 caatcgctaa aagatataaa caagagaaag gaaatacaag atgattggtc tgatttattt
    39061 tgtggtgatc gttctggcga acacagttgg cgctatttcc gggatgggtg gaggcgtgtt
    39121 gatcaaaccg attcttgatc ttatcggcgc ccattctgtt gcagggattt ctttttactc
    39181 aacagttgcg gtattcacga tgtccattgt ttcgactgtg cgacaagtat caagcgggaa
    39241 atcactgaat tggcagattg tcggttgggt atctagtgga gctgttgttg gaggagtagt
    39301 cggcaacatt gtattcgaag tttttttaca gttatttgaa aacgagaagc atgtgcagct
    39361 gatccaaatt tttttgacgg tattgacctt ggtttttgcg ttcttctaca ccaagcatca
    39421 tcagccaaaa tttcatttga cgtcatggac ttggtacctt atatgtgggg ggggttctgg
    39481 gctttttagc tagtttttta ggtattggtg gtggaccagt caatgtttct ttgttgatgc
    39541 tgatgtttgc attgccaata aaagaagcaa cactatattc tctatctacg attttctttt
    39601 ctcaactggc aaaattggta acgattgctt taacaagtag ctttatgcgc tttgatttat
    39661 ctatgttgtt ttatgttatc cccgctgctg ttattggagg tttgtgggga gcaagattta
    39721 gcagggtttt gtctccaaaa aaagtgacct ttatttttca agtaattgtc ctcgtggttc
    39781 ttttggtcaa ttgttacaat gcctttgtca tcttacaaac attaggagaa tctagactaa
    39841 cgcaagtcaa aatcactgaa acatattgtt ctaaatgaaa aaaagagtat actatgataa
    39901 aaaaattcat tttaaaaaat cagaaattgg ctggtgattt ggatgttacg ggaaatgcgt
    39961 ttagaagatg cagaagcact acgtgaaatc aatgcggatc aattagggta tgatatacca
    40021 ttaaccgtta cggtgagaca aatgaagaaa ctgctgaagg agccagagaa aaattttttc
    40081 ttggtttatg aagatcctac ttctaaaaaa gcagctggct atgtacaagc ggagtcctat
    40141 gaatcgattt ttagcgatcc aatgttcaat attatggcac ttgctgtgtc taaatatgct
    40201 gaaaaaagag gcatcggaaa ggctttgatg actggcgtag aagaagaagc gaaaaaacga
    40261 ggaataacga caataaggct taattctgca gaatacagag tagaagcaca ccaattttat
    40321 gaacacatcg gttatcattc cgataaaatg caaaaaagat ttttgaaaat cctgtaaatc
    40381 tttatcaaaa aaatacccga tcaccaagcc gatacttgat gatcaggtat ttttgttttt
    40441 ttgactaaga aagtaattgc atcaagaaac cagagatgat caaaacgatg gcaccaccaa
    40501 gtcgattgcc catttgagca aaagcaataa gttccatccg attagctgca gataagacgg
    40561 ctacatttcc tgttccgccc atcgagttgt tgcataagcc agcagtaata gttgattcaa
    40621 ttggatataa accaaacaaa cgaccgacaa atcctgaaac aactgaaatc gcgataacac
    40681 ttgtcagaca taagacaaca aattgccatg tcaaagcaga agctaataca ttcagattca
    40741 gcaacgcaat accgataccg gcaagaacag ctgctgtcaa gtttttgaca atcaagttat
    40801 tgaacatgat agctgcatct tcataatact ctggtaatag gttggcgatt ttacagatga
    40861 caacgataat aatcataaat gcgtaagcat ggactttagg aacaaaatag ttaccaattt
    40921 gtcctaagat gaagaaagtc aatgatacca tcaaaccaac acctaactgt ggaatggaag
    40981 gtttcacagt tcgaggtttt ccatcgccaa gatcgccttt ttccattaat ttaccattgc
    41041 cgttgaattt tggcattgat tgtccagcac gagcaactaa tgctgcaccg atgatagcta
    41101 agacatttgt cattgctgtc gcaggaatca ggcgggaaat gatagaagaa gcatccgtac
    41161 ctaaaacatt tgcataaatc gttgataaag gaacggctcc ggctccaaca ccaccagcca
    41221 tcgcaggtaa tgagatgtat aaaacggaat ttgcaaaacc atttccgata agcatgccca
    41281 cgagtcctac ggcaaaaaag gagacgacca ttgaaataag tgcaacggga ataaaacgaa
    41341 cagaagcttt catcaataga ttacggttca taccaaggat gctaccagta atcaaggcag
    41401 caatataaaa gtcccaaaag cccatattgc tgacaaagtt ttctgtggat ttcactacat
    41461 attcaggtaa gatcccaaat gtagcaatcg cagcagaagc gaagatcgca aaaacggaac
    41521 caccacccaa ataacttttg acgataggga tttttgtccc gataaaatga aataaattac
    41581 cgaaaataac caaaactgcg attggtccaa tcatattcgt cggtaatttc cctaaagcca
    41641 ttgcaatgat caggacaagg accatgatca aataaagtgg caaacttacg ccggtgattt
    41701 ttgtggacca aaaaccttct tttttctttt cctcaactgc agttgcagtt gttgtttttt
    41761 gtgccatttt tacagatcct ttcttattct gtatcatatg aaaaaagcca aagaaaagcg
    41821 acaaaagagc aggagttggc tggccgctgt tttgccgcag tcgctttttt ctttttagac
    41881 gtcatacaaa gtttctgtat gttcttttac tataacgatc ttaatcttct aaagatttat
    41941 attcaggaac ccatttagca gattcaactg cttctttgat atccgtgatt tcttcacgat
    42001 tcaagttttg tgcgacaact gattttgcga cagtttcagc aatcgtttga gaaaattcag
    42061 tgatttttgc tactggaggc aagatagctg ctcctggttt tgttacatca acgattccgc
    42121 ctaatgctcg actagcttga gaaatgattt cagcatttac tcgtgtagct gttgaagcaa
    42181 tcaaaccaag tcctaaacct gggtacatca aagcattatt tgcttgaccg atttggtaag
    42241 ttacaccatt atattcaaca tcagcagcag ggatacctgt tccaactaat gcttttccgt
    42301 ccgtccattc tattaaatct tttgcttttg cttctgccaa ttttgttgga ttagacaaag
    42361 gcatgatgat cggacgtggt gtatgagcag ccatttcttt cacgatttct tcagtgaatg
    42421 ctccaggttg ggtagaagta ccaatcatga ttgtcggatg gatcgctttt acaactgctt
    42481 ctaagtttgt caattcatca gcattagtga aatctgagcg tttgcgagcg aatgggattt
    42541 gaccaggagt caatccttct gtatcttcaa acaataaacc ttgtttatct acttgataga
    42601 aatgttggcg tgcttcttct tcaggtacac cttgacggat catctcatct aaaaggatgt
    42661 ttgcgatacc cacaccagca gtaccagcac caaatgtcaa aattatttga tctttcaatt
    42721 gttcaccgga tatattcaat ccgcctaata cacctgctag gacaacaatt cctgtacctt
    42781 ggatatcgtc gttgaaagta gtgatttgat cttcgtattt ttctaagatc gctgcagcat
    42841 ttcctcgtcc aaaatcttcc caatgcaata acaattctgg gaataattcg gtagctgatt
    42901 gaacgaattg atcgatgaat tcatagtaag tttcgccttc tacacgagca tgacgattgc
    42961 ctaagtataa gggattattc aataattctt cattattagt accagcgtcg atcgatacag
    43021 gcaaaacttg tgctggatta ataccagcag cagcagtata gaccatcaat ttgccgatag
    43081 cgatgtcaac accgttcaca ccccagtcgc ccatacctaa gattccttct gcatcagtca
    43141 caacaatcaa acgaatgtct cgtccatctg ccgcgttctt caaacttgct ttgatgtttt
    43201 ctggttcgtc gattgaaaga aatgcagcat cttgcggttt caagaaaatt tcgttgtatt
    43261 gttcgattga atccgcaacg acaggatcat aaacgacagg catgaattca actaaatgtt
    43321 cgcccatcaa tttataaaac aatgtccgat tcgtattgaa tagattcatt aagaagatac
    43381 gtttttccaa atctgtttgt ttgcttaagt attgaccata agcttgaaca gattgttgtt
    43441 ccagtgtttg gacagtactt ggaagcatac cagtgattcc atattttgcc cgttcttctt
    43501 ttgtaaaagc tgttccttta tttagaaaag gattatttaa taagtttaac cctgtaagca
    43561 tgaatgattc ctcctaagtt ttaatacaaa cgaagtatag aagtcagatg atgatatagt
    43621 caaaaagagc aggtatgata aattttattt atatgtaata tagccaaaag aaatcaaaat
    43681 agcagtattt gttcttttta tatcgacaat ccttgcttga atactatgcg tatctcttat
    43741 atgctagaga aagaaaagta gaagaataag ggatagatgg aatggataaa taggatttct
    43801 gaggaaaaaa gcacatgata gcgttgatag gagtcaccta tgtcaggtga taaaaataca
    43861 taagtagaaa attaagagga ggaagagaaa atgaaagaag ttcaattgat tagaaaaagc
    43921 gaactatcag aaggcggttg caatgcatgt ggcgtagtag aagcgacaag ctatacatta
    43981 aaattagatt ccaatcaagc aataatctct gaactgacag taggcggtct tgtggacagt
    44041 ctggcgcttg ctgaaggttt tactggagaa gatatttatg agatgttttc agaagtccgt
    44101 cagttaaaaa aaggagaaaa atgcatagag gttcatcatg agagcccgaa tgtgaggttc
    44161 aaacgtggtg ataatgagat gatcttcaag aatcatgtgt ctgatcacac tgaactatat
    44221 gaaatcgtga atcaaatact gtctgggatt tttgaattag gaccttatga gttcaaggaa
    44281 gaaaacggta atccaaaatt aaatgaagaa tggcaagaaa cgattgaaac acaaagaaac
    44341 aatccacact tatttcaata gtcttccaca gtatttcgta aaaatgttga tgaaaaagaa
    44401 acaccgtgaa tcagaggtcg tgaaaaacct gccctgcatc aagtaataag aacagccaaa
    44461 caaaaatagc tcctcctatt tttcgtttgg ttgggcttat taccgcagat gcaagttttt
    44521 gaacaccgtt taaggaagag actgtgaagt caacgttctg acctgagtat tagagagaca
    44581 aatggcaaaa tagttcctca tattttgcca ttttttgagc taataccgag ggtcagctga
    44641 ttgaacaccg ttttatcgag aggttgtgaa aaagtcgctt agtttcgagt agtaagaaaa
    44701 attttgtgaa aatggctttc cacatttttg cataaatttg acttaatacc gagaaaatgg
    44761 cttttgaaca ccgtttattc aaaaaaaggg actttgacac tacttttgtc aaagtcccat
    44821 cggtttttta tctattttta ttacaatcat ttcttccaga gtagtgacat ctggaatggc
    44881 tgaaatgtaa acagattatt tgcctgtatc aatgtcgaag agtaatataa aaagtgtaaa
    44941 cgctatcata ttgttcatca ttcaaatttt atagagagaa ggcattgaga tggaaaaaac
    45001 atatcaagca ggtacaaatg aaggaatagt agattttatc aatatggaag atttagaaat
    45061 cgcggcttca caagtgattc cagctggtgg ttatggttac atcagcagtg gtgctggcga
    45121 cttatttact tatcaagaaa atgaacgggc attcaatcat cgtttgatta ttcctcatgt
    45181 attaagagat gtagaattac ccgatacaac tacacatttt gacgaagaaa tgctgactgc
    45241 accaatcatc atggcaccag ttgctgctca tggattagct catgtgaagg cggagaaagc
    45301 ttctgcaaaa ggtgtagcag attttgggac gatctataca gcaagttctt acgcttcttg
    45361 tactttagaa gagataagag aagcaggcgg ggagaaagca ccgcaatggt ttcaatttta
    45421 tatgagcaaa gataatggaa tcaatttgga tattcttgaa gtggccaagc gtaatggagc
    45481 aaaagcaatc gtattgacag cggatgcaac agttggcgga aaccgtgaaa cagaccgcag
    45541 aaacgggttt accttcccgc tgccaatgcc gatcgttcag gcttatcaat caggagtagg
    45601 ccaaacgatg gatgctgtat ataaatcttc caaacaaaaa cttagtccaa aagatgtaga
    45661 atttatcgct gctcattctg atttgccagt ctacgtgaaa ggtgttcaat cagaagaaga
    45721 tgtctatcga tcattagaat ctggtgctgg tggaatttgg gtatctaacc atggtggccg
    45781 tcaattagat ggggggcctg ctgcgtttga ttcgttgcaa tatgtggcag aagcagtaga
    45841 taaacgtgta ccaatcgtgt ttgacagcgg tgtacgacgt ggacaacatg ttttcaaagc
    45901 aatcgcatca ggtgccgatc ttgttgctat tggcagaccc gttatttatg gattgtcttt
    45961 aggaggaagt acaggagtcc atcaagtgtt tgatttcttt aaaacagaat tggaaatggt
    46021 catgcaatta gctggaacac aaacagtaga agatatcaaa aaaataaaat tgcgtgagaa
    46081 tcgttttatt taagaaataa atgacgaaaa aaataggagt gcggcagatg atgccgtact
    46141 cctattttta tagttcaagg atgtcactaa tagaataatt tgaaacgaaa aaataaaatt
    46201 gaaatatttt ttcatactta ataaaaaagg aagaatattc atgtaaacga ttttattgtt
    46261 tttttctctt ttatacaaaa agggtcataa tttcaagtaa gcggatgaat tcatgaggat
    46321 tttttgcata ctgtctctac tatacttagg tcactaagaa aaaggagtga tttattatga
    46381 aaaagaacat gattgggaca ttattacttt caacacttct tctaggcggc ttggcaacac
    46441 cagcttttgc tgagggtcaa gcgacttcaa aaggagatat tacctttact gaaccaacaa
    46501 ataccgttga gcctcttaac cctactgatc cttcaaaacc agtagagcca gcagatccgg
    46561 aaaatccagc aactggccag acaggttctt taacattgga tgtagttcct gaattgccat
    46621 ttggtacaca cgagattgaa agcggaacaa aaacgtatca agtagacgct tcaaaaaacg
    46681 atacacctta tctccaagta tcagatcgtc gaggtgtagg tgcagatggc caagcacaag
    46741 ggtggaacgt gacagtttcg gtttcagatt ttgtaaacgg ttcacaagtt ttgcaaggcg
    46801 ctgaattaga ttttggaaca tccactgtta aaagtacgtc agataacgaa tccactggac
    46861 caactagtca aactgtaact gggttgagta aagcaagtgc agcgacacca atatttacag
    46921 cagcaaaaga tcaaggtctg ggaacatggc tttctgttta tgatccagca aatatcacac
    46981 tgaaagtgcc gaaagcagca gctggaacat tcacggctga tttgacttgg aacctcgtgg
    47041 ctggcccagt agcatagagt atgaaataaa ttcaacaaac tgcggtgaaa atactatagg
    47101 tgaaggattt tttaccgcag ttgttgttga aaggatgtgg tggacaatga atcaaagtaa
    47161 acgactgttt gtttcgtttt tttctatttt aagtctattt ttcatttttc cgtcaatcag
    47221 ccaagcaaag gattcagcag gagactttgg aatcaaaccg gtatttccag agaaccaaat
    47281 agataaagca atcggttatt ttgatttatt agtggcaccg gaacaaaatc agacactaga
    47341 agtaattatc agcaattctt cagatgaaga acgaacattt gaggtctctg tcaatccggc
    47401 ggtaacgagt gatggtggaa cgatcgatta tagtcaaaaa aacccaacgt tagatgagac
    47461 attgccgttt gatgtccgtg acgttttgct cattgcgaaa aaggaaatca atgtatcagc
    47521 acatgcagaa acaactatac ctatagaagt gaaaatccca gcaaaaagct ttaagggacg
    47581 tgtattagcg ggtattcatg tttcacctaa agaagaagct gaaacagaaa atgctacaga
    47641 aggagcacaa atcaaaaatc gtattgctta taatctcgca gtcgttttac aagaaagtca
    47701 agagacgata gaaccggatc tgaaactttt atcaggagat ttggacgagg tcaacgcaaa
    47761 gccaactgtt cagcttcgtt tccaaaatcc tcagcctagg attatcagca atttgatatt
    47821 tacgagcaag atcttttatg aaaatcagct ttatattgaa aatacgtcta atgaattttt
    47881 agttgcacca aattcgaatt ttcacttgaa tcttgattta gctggtgata aagcaaaagc
    47941 cggcgattac cgcgcagaaa tcatagcaaa atcaggcgac agtaatgaat ggcgatttac
    48001 acaaaacttt acgatcaaaa aagaaaaagc acaaaaagta aatgaaaatt ccgtttttgc
    48061 agtacaagaa caatctttcc catggcttta tgcaggattg gttgctgcag ttctagtgtt
    48121 atggttgatt ttgctgtttt tgtgggtcca caagaaaaag aaaaaaggag aaacaacatg
    48181 atgaggtgat ttcgttgaaa acatcgatct tattgggagg gattattgca agtttgctgt
    48241 tcttcagctg gcaagagcca gtccaagcag ataccaaaag ttcaggtacg attacatttt
    48301 atgatctaag tccagttccg catccaagtg gttcagtcaa tggcgcatca aaacagcaga
    48361 cacagctacc gatttataaa gaatctaatc aacatggcgt attcccggag acgaatgaag
    48421 aaaagatccg ttttctttat ttgatcattg gtatagaatt atggcttctt gcgtggttgt
    48481 tgttatggag aagaaaggaa aaggaggatg aacatgttgc tacaaaataa attttcattt
    48541 tttcgctcct tcttttttac ttcgcttttt gttttaagta tatttatctt ttcaccttcg
    48601 gcagaagctg ttggagcaac gcctcctgcc atttcttatg atccatacac gtatgaaaat
    48661 atcgtacctc caaatggtgg acgtagtgca gtcaatggcg ggaagcttgt ccaactaact
    48721 ggggctaacc aattgcctaa attgactgct acttatttga acgatggttc aaatgatcct
    48781 tggaaaggtg gcacggtccg ttttcattgg gaaagccatg tgaaactaac aaatgtactt
    48841 gatttggtga atatcactgc acaaggattt gcatttgtgg cgatcatcaa tttgccggaa
    48901 catgtatcaa gcagtgatgt attaaaagca attgactgga acaacgccta cttgtatatt
    48961 gccggtgccc ccatcaaggt tactagcggt agtttattat cagcaggaag ccataccttg
    49021 cgttttggga tggggtcgtg ggatacaaat actctattgg cagatattat tgctattatt
    49081 acgcgaggcg aattcaacgt ggacgatatc ccgcttcaat ttgatgttac agctaatgtc
    49141 gcgaagatga cagaaaacgg agataaatat gatacgagtc caaacaaaat tttgaccaag
    49201 ggtaaattac aaccttctat gtctaaaaag gccgtttttt ctgttgattt ttatgattcg
    49261 aataatatta tccaagatcg tatgttcaat tcgattttag ctccagctgc aggtagattt
    49321 tatcctaaat taactggcgg aaacttgaat ccaggagcat atgcattttc tgcaagtgca
    49381 gagataaata cgtggaatag ttacatctca acaagtgatt caacaggaaa atataataca
    49441 tttgctgata ccacagaata cgtggatgga aataaccaga accttccagg ctctaccaat
    49501 agtcggacat tgacgatggg gctgactgaa ggacgttttg atcagttgcc atttagtcgc
    49561 tttgatcgcg tagtcaacta ttttacagga aaaaacgtta cacaaggagc aatactgact
    49621 catactccag caagcacaag tggattgaat cagacagcca atatcgtgta cagtgggaaa
    49681 gatgcgcaag gcggattgct ttctcctgtt gctttaaagg tattggagca gtacaagcta
    49741 gacggaacgg ttttaccaac aaacttaaca gccgatagtc agtggggaaa gctcctttct
    49801 tctgcatcgt accaagtcac ggccaattgg tcagcaactg aactaaaaaa gggttcgatc
    49861 cattacaggc tttattcgaa aggaacccaa aaattagcga caggatggac agatcaactt
    49921 ttccaaacga tccaatcaaa taacgggggt gtaaatacag ctacttctac actacctgca
    49981 ttggcttcag ggcaatatta ttttgattat cgcttggtgg acgatgtact tactaacatg
    50041 tatccgtctt ttgcgtacaa atggcagtct gaacaagctg gaatcaccac tcttcctcaa
    50101 ataacagtgg ctaattttcc atctgtgagt gcagatagtc agctaaaaaa ccttagcaga
    50161 caacctgaga caggagatcc cctgtctgct ctatcaggag atacgattca agaaaatgtg
    50221 acattcacgt taacaaaatc tggggatgcg ataaccgaca aaaaaatcac gatctcactt
    50281 cctgaaaata ctacttatat caatggttcc ttaaaattaa atggtacaac tatcgcagat
    50341 accggcattc aacaaggagt ttctgtccca gcagatctat tgagcaaaat aggagatacc
    50401 attcatctta cttacaacta tcaattaaat actgtagata cttctgtaca atccgtcagc
    50461 atactgacaa aggcagctgt actttccagc aatattacat tagcagatgg tgctaagctt
    50521 cctaacccag ttgtccaaac atctgctaaa acaattcttg tccccaaaca agaattaaca
    50581 ttagttaatg taccagatga ttttacattt ggtaacgatc tccccaaacc gttgaagaca
    50641 agttattatg aagcaaaagg cgatttttct tttgatgtgc gggatactcg gcttccaagc
    50701 acgtctcctt ggcaactgac agggacattg acttctttat ttaagaatga tcaaggtcaa
    50761 gaattaagcg gaacaaaatt atacttcaat cattctggat caaaacaatt gattcagcaa
    50821 ggtcaaaaca cgttgattta tgagagtgat ggaacagcta aaggagaggt tctcgttgac
    50881 tttcccgata ccgatggttt actcttagag gtaaatagca gtacgaatgc tcaacctggc
    50941 gcaacgtatc aaggcatggt cacatgggaa ctgacagctg ggccaaccag ctaagtgatt
    51001 agaatttgta tggatctgtg ataactgtca cagatcctct ttttcaagga tgagaaggaa
    51061 acttctgctg ttaaaatcag aaatgcttgg aggagtggac ttggaacttt tcagtttgct
    51121 tgaaaaaaat gaacaacggc aaatcaaact tttccaactt ttattagagg aaaaaggaga
    51181 gatttccgtt aataacgtac ttacccgctt atcagttgat cgggctacct tgaaagaaga
    51241 tctgttttat ttacaagcta acttggaaaa tatcaaacaa ttggaaattg ttatccaaga
    51301 agggaatatt ttattaatca gacatggaaa tatagcagca gtcgatgttt attatcattg
    51361 ctacttgcgc tattctacaa aatatcagat acttctttat ctgttggaaa atggctcatt
    51421 tgatcggcag aaacttgtca tggaacttaa tatcagttcg gctactttgt ctcgcagagt
    51481 gaaggaatta aatgattctt taaatgagtt tcatctgaaa ataaaaagcg ggcggctgac
    51541 cggtccagaa agtcaaattt gttattttta tttccaatta ttttggtttg gacgtcctta
    51601 tcttgaaaac aaaaaagaat acagtgattt atcaggaaac acttttttag atctgctgca
    51661 aaaagagttt agttttacta ttacagaaga aggacgattg aaatttgaaa tatggctttc
    51721 cattatgaag aagcgaatgc gctgtacgaa aaaatccaaa ccagattata gcaatcttcc
    51781 tgttggattg tttcaaacga cacaattttt attggacctc catacatttt tgacccgtta
    51841 cttctttcaa tatgcctttc cttggaatca aaatgaaacg ttggtttttt atcttttcat
    51901 gatcagtaac tttacattag atacacataa ctcgcatgtc caggagtttt tgataagcga
    51961 aaaatacaaa gacgacgtag tagctcaatt gaatcgaacg tttaaacaat cgatagaaga
    52021 atatttctct ttttatgttt ttccagaaga gatttcgcaa aagattgcgt tgacagtgat
    52081 gcaaaatcac gctcattttg cctattttag aggatggatg aatatttttg gccaagaagg
    52141 attaaaacag cggttaactg aagtagaaga taaacaattc ctggttttat gtgaaagatt
    52201 ggtcagacaa acatttgcta ttgttgattt tactgaagta gacgaggaaa atgcccgctt
    52261 tgaattattt gctcgctatg catctatttt gcggacgctg atcaagcagt tagaattacg
    52321 tctgcaaatt gcttatgatc ttccttatga aaggctaatg gcggatatcg tgaaagaaat
    52381 catccaggaa caattgctga atacgatcaa atatgattta gtcgaatacg agaaagaaaa
    52441 gcagtatgat atcattttga ctagccaatt gaaagagtac ccgtcacaga agtccgctaa
    52501 agtttttgta ttcactagca acgaatataa ttatgatttc ccctatttga accaattttt
    52561 aaaagaatgt tatttagaaa aattgaattt aaggatgaat aaaacatgaa aatcgtgatc
    52621 attggtgctt cccatgcagg catcacagct gccctaaatc taagaaaact tcaaccgaaa
    52681 gcagaagttt tactgatcga tgaagatcat aaagatggac taggctatgt ttcaaatgga
    52741 atcaaccttt atttaaaagg aaagatacgt agtttagctg aagttgccca taacatgcgg
    52801 actcttcaag aaagtggggc aaaactgatc accgaatgga gagtaactga acttgatcca
    52861 gacaaacatc agctcatcct cgcatccaaa gaaggaaaaa acgagaccat aacctatgat
    52921 aaattaatcg tagcaacagg ttcctctcct gtcaccttgt ataaacaaat tgaagctgaa
    52981 aatgtctaca cctataaaaa tctagtgcaa tctaagcaag tacttgccgc tttaaaagaa
    53041 gcaaaagaag tagtgatctt cggtgctggt tatattggat tggaattagc agatgctttg
    53101 agaaataaag gatacattat tcatctagtt gattatatgc cgaatgtttt atcaagatat
    53161 ttcgataagg atatgatcaa cagttttcaa aatcagctgc aaactaagca aatcaatttt
    53221 tatccaaatg aattcttgat cgattggaaa aaatcagaag agaaagtagt atccgtacaa
    53281 cttctatctc aagcaataaa ggcagatatg gtcatttttt ctgcccaaac tcgtcctaat
    53341 acgacattgt taaaagaaaa agtggctttg tatgaggatg agactgtgat agtcaatgaa
    53401 tatctgcaga caagtgatcc ggacatctat gcgattggag atatcgtccc agtcagtttt
    53461 gacaaaaata aacggcatct ctttttacct ttagttactc gagctgtaca tatggcaaga
    53521 gcagtagcgc taactttgag tggtcagcca accgcttacg acctcagaca aaagataaca
    53581 gcagcagtca ttatagacta ctttttagga actgtagggc tgacagaaga tgaagcgcca
    53641 ttcttggaac aaaataccgg ttcgtgttca ggagaatttg atctatttcc acaatacgat
    53701 gaagaaaaca aaacagtaaa tgcaaagctg atctaccatc cggatacatt agaaataatt
    53761 ggcggacagc tgataagcca agaattctta ttatcagatt tgaacctgct tgcggatatc
    53821 gtgaaacaca aaactacgat tccacagcta gcagttgagg attttggatt tctgtctgaa
    53881 cacacctctc gttttcacta cctcaatgag ttagctttta aagtactagc taagagagca
    53941 aatagcaata gagtagataa aagactgtga gaaatatcac ggtcttttat tgtgtgggga
    54001 tataataaat attaatcaat cgagaataaa aaattcatat aatgaaatag cggaagaatt
    54061 ttttgagcat aatattcgcc aaaaatttat caagtgataa aattgttgta taaaatgatt
    54121 atttcaggag gaggagttaa atgtttttag cattaaatga aattatgcat tcaaagttac
    54181 gttatgcgtt agttgcaggg gtgatgtttt tgatcgccta cctggtattt tttttgactg
    54241 ggttagctta tggcttagct caagataatc gaacagcagt cgacaagtgg gaggcagatt
    54301 cgattgtgct ttcgaaagat gcaaattcaa atctagggat gtccatgatt actaagaaga
    54361 tagcagaaga agtcgaaggt gggaaagtgg cgtatcttgc ccagacacct ggagtcgtga
    54421 ctagtaaaga cagtacagaa gaaggaaaaa tcaatgttag cttcttcggt atcgataaaa
    54481 atcaattcat catgcccaat cttgttgaag gaaaagcttt tgataacgat gatgaggcag
    54541 taggcgatat tagtttgaaa gaagaatatg gtctagccgt aggagataca gttaagttat
    54601 ctggtagtga caagacgttt aaactaactg gctttacgga tcatgccaaa ttcaatgtct
    54661 caccagttct ctatacaacc atcaatgctt accaagagat ccgcttcgaa aaagaagata
    54721 caagtgaaaa tgcacggatt aacgcaatag ttgtcagagg taaaatcagt aatttacctg
    54781 aagatttaga acagatcaaa atttctaaat tcatcaatga attacctgga tataatgcgc
    54841 aagtattgac ttttggattt atgatcggat ttttgatcgt tattgcggct attgtgatcg
    54901 ggattttcat ttatgtactg acgatgcaaa agattaatat ctttggtgta atgaaagcac
    54961 aagggatcac aggaggattt attgcgcgtt ctgtagtggc acaaacattc attttatcat
    55021 ttgttggtat tcttcttggt ctattaggaa cagtgggaac atcccttgtt ctacctgatg
    55081 cggttccatt ccaaagcaat tggctgtttt ttggcgtcat tagcttgttg atgctggtag
    55141 tagctgtgtt aggcgcacta ttttctgttc ggacaatcgt aaaaatcgat ccgttgaagg
    55201 caattggata agaaggagga agagcatgaa agcaatagaa tttaaagaag tagaaaaaag
    55261 tttccaagat ggcgaccaag tagttaaagc attgaaagaa acaaccatga gtatcgataa
    55321 aggggaattt gttgcaatca tcggtccttc tggttcaggt aaaagtactt ttttgacttt
    55381 ggcaggcgga ttgcagacgc ctagcagtgg ctctgtattt atcaatgatc aagaattcag
    55441 taatcaaaaa gaaagtgaac gcgttaaact acgttttaaa gaaattggct ttattttgca
    55501 agcttccaat ctggttcctt ttctgacagt aaaaaagcag ttattattag tagataagat
    55561 caaacatgaa catcgtcaaa aagaagcaaa agaattattt gagcagcttg gcgtggataa
    55621 gttgatcaac aagtatccag aagaactatc tggtggagaa cggcaaaggg tcgcaatcgc
    55681 tcgtgcattg tacaatgatc cgtccattat tctagcagat gaaccaactg ctagtttgga
    55741 ttcagaaaag gcacgagaag tagtcgatat cttggcaaaa gaatcaaaag acaaaaataa
    55801 agcaacgatc atggttactc acgatacacg gctgattgac aaatgcgata gaatatttat
    55861 gatggaagat ggtgtattga aagaaaaaga ataaccaaaa agcaggtgga aaaatggtcc
    55921 atcgatacaa caaagataca cagcaaaaaa ttttgaaaga agcgactgac ttgtttatgt
    55981 ccaagggata tctcggcact tcaacaagag agatcgcaca aaaagttggg atcacccaac
    56041 ccaatctcta tcattatttc ggtgataaag aaaaactata tacggcagtt ttagaaaacc
    56101 acttaaaaga tgttggaaaa gctttgagag aaatcgttca aacaagcgaa acgggatttc
    56161 aagtaacctt gacaaaaatg gcgcagtttt tgattgagac acatctggtc gatttgtttc
    56221 tgatgcttca cgatcttgaa agcaatcttt caaaggaaac aagagatcat cttttctttc
    56281 tatggaagaa aaattatcgt gaaccttttg aagaaatctt ttcagcaaac caatctgtgc
    56341 taagaaatgg gatttcaaaa gaaatcgcag caaggcattt tttcttagtg ttagcacctt
    56401 acatcactcg gtcgaaaaga cctgtggaag aaccgctaaa tgtggcgcaa ttgatcgatt
    56461 tatatttgaa tggtgtatta ggagaagcgt aaagctgtta tatagggaga aaaatagtta
    56521 ggtgaagaag caagatatga cgaagatcgt tatatcttgt tttttttata taggtagttt
    56581 ttcataaata caaatttgtt attctctcat gattatcagg gtatattttt atttattatg
    56641 tcggtgttcc aggaagtgtt ttaatttaat aacgaagaaa ttaattgttt gtagaatgaa
    56701 ggaaactatt tattgggtag atgaatcacc acgaatagtc aaaaggagat ttcaggatat
    56761 gccaaaaatg aaaaaaatca ttttagcagt aatgttattt tctacggtta tatcttttac
    56821 agcccctttt ttgcgtactt aagctgattt taagttttga cccttagtct ggatctacga
    56881 ggtgagagtc aagaatgaaa aaaataattg gagcttttgt atttgcaatc attatcatta
    56941 ttatctgttt tcaaatagat gtttttggtc aaaaaaagat agttggacaa acggtcagca
    57001 gtacgaacag tacggttaca aagacaaagg aatcatttga tgatacagaa caagagacat
    57061 taatcaaaat cggaaaaaat gaccgtacac tatatacaga tgactcgcta actaccccac
    57121 ttgcaactat aaacggcgga gagctggcgg agtttctttc tgaaacaaaa aatgcttatc
    57181 agatcaagac aaatgatggc tatactggat atttagccct cgaagatgga accaaagtca
    57241 caaagaatat acaaacaaaa ccggaagagc tatctgatgc agtgattgta ttagatcctg
    57301 ggcatggcgg aaatgatact ggggcattaa gcaatgatga acaaacagaa gaaaaagata
    57361 taacattaag tacagccata aaagtaaaaa aggcgctaga ggatgctgga gcaacggttt
    57421 atttgaccca tactacagat gaattagtac agcttggaga tatatgcgac tacagtgaag
    57481 aaaaaaaggc agatgtcttt atcagtttac acgcagactc tacggaatat gcgaatgaag
    57541 caactggaat cacgacatat tattactatg gacaagagga aacattagca cagacaattg
    57601 ctgactcctt cactgatctg cctctggatt caagaggaat ctccactggg aactatcaag
    57661 ttcttcgaga aaatcttcaa ccttctattt taatcgagat gggttatatg aataatgata
    57721 gtgatttaaa ggaattagta acagacagtt atcagcagca gattgctgca agtttgacac
    57781 aagcattgac cacttatttt cagtaataaa taagcccagc caaacaaaaa tagctcctca
    57841 tatttttcgt ttggccgggc ttattatcgc agatgcaagc ttttgaacac cgtttaagga
    57901 agaggttgtg agcctgccgt ttaactccgc gtcacaagga gcaatttcaa aaaacagctt
    57961 ctcatgtttg atgactattg tgacttgtga ccgaggagtt ggcatgagaa caccgtttat
    58021 tcggaacaag ggactgtgac atgacttttg tcacagttcc attatttttt tagcagcttc
    58081 aaagcttttt ctgtattggc aaaatgaagt ttggcatatt tttcaagcat aggcatgccg
    58141 cctttctcca atagttttgc cgctacttgg tcagatttcg ttccagctcc tgatggaagg
    58201 gtgattccag cttcagctga tgctttgtgt aactcttcca aaaagctggc acgactgatg
    58261 atagaagcag cagcaacagc taaatgatat tgttctcctt tagtaacgaa gtacagtttt
    58321 tcttgtaatt gatttttttc taaacgcgca tattttttaa aatttgcttc cggcgtaaac
    58381 tgatcgatca aaatcgcttc tggtttttct ggctcgattt tttttagcag caaatggatc
    58441 gcttgattgt gcaaagcgac tttcatccga acggcattgt attttggttg gatctcattg
    58501 tatttttctg gagataagac aagcagttta taggggatgg ttttttttaa gatgtcagca
    58561 agctggatga tttggctatc ttttaactct tttgaatctc taacaccaag tttacgtaat
    58621 tctgcaagct gatcacgttt cacataagca gcacatactg ttagcggacc aaaatagctg
    58681 ccatttccga cctcatcact acctaaaacg gataattgac tgatattttt tggcaaagaa
    58741 gaggctgttt ttgctggctt ggcttgttta tcctgtgcct ccccccattt atcagcttct
    58801 attgctgcat tcgtaccttg aaacatcact tttccggatg tataagcggt aatggtcgtc
    58861 ccgtcttttt ttgcagcaaa gctagtataa ggaatctgtt tatttaacaa ataagagcga
    58921 tagtatgctt tcatttgact aagttggtcg tttgttgctt tgataatgac gttgctcata
    58981 aaattctcct cgtttttata cataaaacta taccgcaact cacgactttt cgttttctaa
    59041 gagattgcga gacatagcat ttttttaaag tttatcatat aatagttaag tatgtgaaaa
    59101 atatttgtgg agaacggtga aagaaccaac ttcatggtac actaaataaa actttcatca
    59161 aataggagga aatgattcgt atggcgcacg aaaaaacacg ctacaaagca gtgattgcca
    59221 accaaacata tactattatc ggacgggaaa ccaagcatca tatggatatc gtaacgaagc
    59281 tgatcaatga acagttagca gaattgaaac aattatcacc acaaatggat aatgaacaag
    59341 cagcaatctt gatggctgtc aatgcgcttt cggatcaatt gaagaaacaa gaaagaatct
    59401 tggagctgga agaagaaaca gcagaattaa agaaaaaaat gatcaaattt acagagcttg
    59461 aaaatcgtgt gaaacgcatt gaagcaatcg agaacgaagc tcgggaagta ttgaaagaaa
    59521 atggtcaagc agatcatacc attaaaaatc atgtggaagc acaacagatt ttaaacgaga
    59581 aacgaaaagg acaaattaaa caaaaatctt ctaattaaga tccttttatc aaaataagaa
    59641 aaatttaaaa aagttagaag agaaaggaac gtagcatgct gagtttgctg attttatttt
    59701 tattgctaat tgcgtttttt tctggagcac gtcgcgggtt tgctttgcaa atcatattcg
    59761 cgattggata tgtattgtca tttattgctg cccagcattt ttacaaacca ttagcaagtc
    59821 atttggaatt atacattcca tacccagctg tcaccccaac atcaaagtta gcattttttg
    59881 atcaggtatt tgcttttcac ctagatgaag ctttttatgc aggaactgct tttctgatta
    59941 ttttgctaat tggctggctg cttacccgat ttgtgggggt ttttgtccat ggattgacgt
    60001 atgtgccgat cttgagacag gtagactgga ttgcaggagg catcctgtca ctaatcatgg
    60061 cttacgttac tatatttttg attttgcagt tacttgcctt tgttccatta gtgtggtcca
    60121 aaatcaattc ggcgagcata gtttcgctcg ttttatcgtt gagagaacgc catttcttac
    60181 caacaagatg tatgagcttt gggtaacgaa tattttgaat taaggaaaat agtgctggaa
    60241 aatatttcgt tttttgtaca gagtagttta tttaagaagg ctgcgtagcg tatgtccggc
    60301 acagctcttt tttcatagtg aaagataagg tgataaaatg aataaccgaa tccttgaaac
    60361 attggaattt gaaaaagtca aacagatggt cagacaattt gtagtgactg cacaaggaaa
    60421 agaagaactg gcagaattgg taccagtgag tgagaaacaa acgatcacaa actggcttca
    60481 agaaactgag gatggattga aagtgcaacg tctgcgaggc gggatcccta tacctaaact
    60541 tgaaaatatc cgtccccata tgaaacggat cgagatcgga gcagacttga atggagtgga
    60601 gcttgcacaa gtttccagag tattgtctac gacaagcgaa ttgaatcgtt tcattgatga
    60661 tctttctgac agtgaaatcg aatttgcacg tttgtatatg tgggcagatc agttagtcac
    60721 gatccctgtt ttgagccgcc gtttaaagga agcaatcgat gaagacggac gcgtgactga
    60781 tgacgcttct cctgagttaa agagcataag acagaacatt cggcgaagtg aacaagctgt
    60841 tcgtgaacag ttagatggga tcgtccgtgg gaaaaatgca aagtatttaa gtgatgcgat
    60901 catcaccatg cgaaatgacc gctacgttat cccagtcaaa caagaatacc gcggtgtatt
    60961 tggcggtgta gtccacgatc aaagtgcttc aggacagact ttatttattg aaccaaaaca
    61021 agtggtggat ttgaataatc gtttgcgtca ataccaaatc gctgaacgaa atgagattca
    61081 acgtatttta agtgaacttt ctgctgaact tgttccccat cgtcaagaaa tcatccacaa
    61141 tgcttatgtg attggtaaaa tggatctgat gaatgcaaaa gcacgatttg gtaaagaagt
    61201 aaaagccatc gtgccgggaa tcagtgaaga caatcatgtc gtcttgaaac aagcaagaca
    61261 tccactgatc gatcaggaaa aagtagtttc taatgacatc acaatcggaa aagactatca
    61321 agccatcgtc attacaggac ctaacacagg tgggaaaacg atcacgttga agactttagg
    61381 tctgttacaa ctgatggggc aagcaggatt accgattcct gcaggtgaag aaagtcaaat
    61441 cggtattttt gaggaagtat ttgcggatat aggagacgaa cagtcgattg agcaaagtct
    61501 atcgaccttt tcttctcata tgacgaacac tgtagatatt ttatcaaaag tcaatgaaaa
    61561 aagtctggta ttatttgacg agctcggtgc aggaacagat ccgcaagaag gtgctgctct
    61621 agcaatcgca atcttagatg atcttggtaa aaagtctgct tatgtgatgg caacaacgca
    61681 ttatcctgaa ttgaaagttt atggatacaa tcgggcaaat acgattaatg ccagcatgga
    61741 gttcgatgtg gatacactaa gtccaacgta tcgtttgctg attggcgttc ctggacgaag
    61801 caatgcgttt gaaatctcta gccgattagg tcttgataca gaagttattg atgaagcgaa
    61861 gcagttgatg aatgatgaga gccaagattt aaacgaaatg atcacagatt tagagaatcg
    61921 ccggaaaatg gcagagaccg aatatctgga aatgcgtcat tttgtttctg aggcacaaga
    61981 acttcacgat gatttgaaag aagcttacag ctatttcttt gaagaacgtg aaaaagaaat
    62041 ggaaaaagca aagaaaaaag caaacgaagt cgtgtcagag gcggaagaaa aagcagaaaa
    62101 aatcattgct gatattcgta aaatgcaaca gcagattggg caaggaaatg tgaaagagca
    62161 tcagttgatc gatgcaaaga cccaattagc aaatctacat caagaagaaa ctttgaagaa
    62221 aaataaagta ttgaagaagg cgaaagaaca aaaaacgttg aaaccgggag acgaagtgtt
    62281 ggttacgact tacggccaaa gagggacgct tttacgtaaa aacgggaatc agtggcaagt
    62341 agaaatcggt atattaaaaa tgaatgtgtc cgaagacgag ctgacgcctg ttgctcctca
    62401 aaaagagccg acacaacgag ttatccatgc tgtacgatct gaatctagta gtcacgtgcc
    62461 gaatcaattg gaccttcgag gaaaacgata cgaagaagcg ctaagtgagg tagatcaata
    62521 tttggattct gccattctag ctggttatcc tcaagtgact attgtccacg gaaaaggaac
    62581 aggcgcactt cgcaagggaa ttactgatta tcttaaaaac catcggagcg tcaaaagctt
    62641 tgagtttgca ccggcaaacc aaggcggaaa tggtgcgaca attgttaaat ttaagtaaag
    62701 cttactaaag gtaagcagat ggttcgctat ccaagtatag aaatgctata ctaatcatag
    62761 attttaaaaa tggaggtctt tgatatgtca caagtaatta cagataagac atttaatgaa
    62821 gaaacagata aaggacttgt attgattgat ttttgggcaa cttggtgtgg tccatgtcgt
    62881 atgcaagcac cgattttgga tcagttggaa caagaatatg atgaagaaga attcagaatt
    62941 gctaaaatgg atgtggatga aaatccagaa actcctcaac aattcggtat catgagcatt
    63001 ccaacattaa tgttgaaaaa agatggtcaa gtagtagaaa aagctgttgg tgttcattca
    63061 aaagaacaac tacgtcaaat gatcgatcaa tatttataaa aacttacagc aaaaataagc
    63121 tgagggtggg actgaatcca gttccgtcct cggttttttt gcggttgagt taaggttatt
    63181 caaattaata ttttttgagt ataattacgt tgtcaggctg gattgttttt ttgacagaca
    63241 tgctataata actttaattg aatatgaagg ggagtaccag atgaacgaac gaatcaaaaa
    63301 caaattagct ttacttcctg atcagcccgg ctgttacttg atgaaagata aaaatggaac
    63361 gatcatttat gtcggcaaag ccaaaatatt aaaaaatcgt gtccgctcat attttactgg
    63421 cagccataat acgaaaacgg aacggcttgt tagtgaaatc gaagactttg aatacatcgt
    63481 tacagaatcg aatatcgaag cattgctctt agagatcaat ctgataaaga aaaatgatcc
    63541 taaatacaac attatgttaa aggatgataa aacctatcct ttccttaaga taacgaatga
    63601 aaaatatccc cgtttggtga ttactcgtaa agtactaaaa gacaaagcac tgtattttgg
    63661 accatatccc gatgtcggtg cggcgaatga gacaaagaag atcctcgatc gattatttcc
    63721 tctgagaaaa tgcaaaccta gtcagacgaa ggagccttgt ctttactatc atttgggaca
    63781 atgtctgtgt ccttattatt tcgacgtaga tccgaaagtc tatacgaaaa tcgttgatga
    63841 agtgaaaaag tttttaaacg gaggacacga aaaaatcgag tcagagatcc atcagaaaat
    63901 ggaaaaagct gctgaaaaca tggagtttga aaaagctgca gaataccgag accagatccg
    63961 tgccattgag acaatcatga ctcgccaaaa aatgacaaat actgatttac tggatcgaga
    64021 tgtatttggt tatgcgattg acaaaggatg gatgtgtgtc caagtgtttt tcgtaagaca
    64081 aggaaagcta attgaacgag atgtttcaat gtttcctttc tataacgaag cagaagaaga
    64141 tttcttgact tacctcggtc agttttatca ggaaaatgag catttcattc ctaaagaagt
    64201 cttgattcct gatacgatcg acaagcccag tgtagaagct ttgttagcta cgaaagtgct
    64261 gcaaccaaaa cgaggagaaa agaaaaaatt agttgaattg gcgaataaga atgcctcagt
    64321 tgctttgaat gaacggtttg atctgattgc cagaaaacag gaacggacga tcggagcagt
    64381 tgaacgttta ggaaacgcga tgaatatccc gaccccaata cgtatcgagt cttttgataa
    64441 ctctaatatc atggggacga atcctgtggc tgcgatggtc gtattcattg atggaaaacc
    64501 ggcaagaaaa gaatatcgga aatataaaat aaaaacagtc aacggaccgg atgattatgc
    64561 atcaatgaga gaagtcatct atcgcagata ttcacgagtg attcgcgaag ggttgccttt
    64621 acctgatttg atcttgatcg atggaggaaa aggacaagta gatgtggcaa aagatgtctt
    64681 agaaaatcaa ttaggaatcg atattcctat tgctggcatg gcaaaaaacg ataaacataa
    64741 aacaaatgag ttgctattcg gtatagattt ggctgtagta ccgcttgagc gaaattcacc
    64801 agagtttttc ttactccagc gaatccaaga tgaagtccat cgattcgcaa tcactttcca
    64861 tcggcagcta cgcagtaaaa acagttttgc ttcccgatta gatgaaattg aaaggttagg
    64921 accaaaacga aaaaaaatgc tgcttaaaga gttcaaatcg ttgaaaaata tcacagcagc
    64981 cacgacagaa gaattacaag aaatcggact gccgaaaaat gtcgcacaaa acgtatttga
    65041 taaattacat caggaatgag gaaagggcac tttacaaaga aagtaaagtg tcctatcatt
    65101 ttatgtacaa caaaaagagc gtttacgttt tgttggtaaa taataaatcg cttacgtctg
    65161 gtgtctaagg atatatgtga tttcttgaag aaggagtgaa gaaatgttag aagtcaagga
    65221 tcttgttaaa acttttggga actacacagc tgtcgatcat gtctctttcc aaatccccga
    65281 tggaaaaatc atggggctaa ttggccaaaa cggggctggg aagacaacga cttttcgttt
    65341 gatcttggat tttttgacac aagaccaagg agaagtgtta tggaatggcc atcatcttgg
    65401 ggagaaagat tatgatgtca tcggttatct tccggaggaa cggggacttt atccgaaagt
    65461 aacgattcaa gaccaactgc tttattttgc agaacttaga gggaaatcaa gaaaagagat
    65521 tgaaccaaaa atcgattttt ggatggaaaa atttcaggta aaagggaaaa agacggataa
    65581 agtaaaatct ttgtctaaag gaaatcagca aaaagtccag ctgatcgcca cgttaatcca
    65641 tgaacctaaa ttgatcattt tggatgagcc attcagcggg ttagatccgg taaatgctga
    65701 attgctaaaa gatggaatat tggaattgaa aaaacatggt tcatgtgtca ttttttctag
    65761 ccacaatatg gataatgtag aaaaaatctg cgatcatttg atcatgttgc gaaacggaaa
    65821 aatggttttg aatggaaaag tacatgagat tcgtgaatca tttggccgta cgaagttatt
    65881 cttggaatct ggtttaagcc aacaggaagt agaagaaatc gaaggcgtga aaaaagtgat
    65941 ccttcatgaa gacaatacgt tagagattac cttagaagat cctgaagctg ggaaagaaat
    66001 ctttactcgt gcgactcagt tcggctacat tcctatgttt aaccagcaac cgccaacttt
    66061 ggaagagatt ttcaaattga aagcaggtga aatcgatgcg taagttttgg attattacca
    66121 aagatgtcta tttaaaaaat gttaagtcga tttctttctt gatcatgata ttagtccctt
    66181 ttatcatgct gggtatcatt tatgtagcag gaaactttgc tcaacaaaat agtgatgtca
    66241 ataaaatcgg cgtattggct gataatgagc aaatcagtca gcaaatgagc caagtcaaaa
    66301 cagatgattt tgagttcaaa gcagttgatt ctgaagaaaa agcacaaaaa gagctttcgg
    66361 aagaaaaaat cgatgcgtat ttagtagtct caactgagaa tgataagata aatgggatgc
    66421 tttatagcga aaattcatta ggacaaagta cacaactact gatacaacaa caactgactg
    66481 gcttgcaatc gatgcttcga gcaagtcaat taggcatttc accagaagaa gtagctagct
    66541 tgagccaaca agcaagtttg actcgacaaa aagtcaattt tgattcaaaa ggaaaaatga
    66601 cagttggaga agataactcg acagtccaat atgtcgtaag ttatgtagca acgattgtct
    66661 tgttcattat tattttgacg tatgcgcaga tcattgcaca agagatcgct tctgaaaaag
    66721 gcacgcggat catggaagtg atcctgtcca gtacgactgc acaaaagcat ttttacggaa
    66781 aactaacagg tgttttactt gttgcattaa cgcaaatggc attatacggt attatttttg
    66841 gtataggata cagtcaattc aaagatatgg aaatggtcaa aaatatatta gacggtatct
    66901 cattggataa cttgattgga ccattcttgt ggtattctct tctattcatg atctttggca
    66961 ttttgatttt ttctgtttta gcagctttat gtggctcttt ggtcaacaaa gcagaagata
    67021 cagcaaaagc aattttgcca gtgacttatc tatctctagg cggatatatg cttggattga
    67081 tactaggagc ttcagatcca aacaatatcg ttattcggat cacttcgtat attccattct
    67141 tatcttcata tattatgccg gttcgcttag caaatgagac agtcggtatg ggtggagcat
    67201 tgatttcctt gttggtactt gtagccgtaa cattcttatt gatgatcttg tctgcaaaca
    67261 tgtacaaatc gaatgtactc gtgtatagcg aaggcggatt atggagttca ttgaaacaat
    67321 cgatttcaat tatgagaaat gaacgaaaaa aagcataaaa tatagaaaaa tatagaagga
    67381 agctggacat ttattgttcc cagcttcttt tttgtataga cctatttcta tttttttatt
    67441 atattggata tatatattca ttgaaaatag agcataacaa ctttataatg ttgagtatac
    67501 caatttttgg aggcgtttta atggaccgct atttagattt actttcagag aagttcccta
    67561 caacggaaga tgtcatcacg gaaatcatca atctcgaagc aattttgcag cttccaaaag
    67621 gaacagagtt atttttgagc gatatacatg gcgaatttcc tgcttttgat catattctgc
    67681 gtatcggttc aggaaatctt aaagaaaaga tcaaagacct tttctctgaa caaatgacgg
    67741 aaacggagat cagccaattc actattttta cggcttatcc agaatatgtg ttgacgactg
    67801 attggtacaa aaaacaagat aaatcccagc tgatccatcg tttgatcgat cttttacggt
    67861 ttacgacagt caaatacact cgatcaaaag ttcgaaaagg attgccgaaa gaatatagct
    67921 atatcattga ggaactcttg tatatcgatg atcgtatcaa cgggaaaaaa gactatgtaa
    67981 aaaaaatcat tgaacaactt gtcttgatga aggaagaaga aaaattttta acaaaacttg
    68041 cgcagacgat ccaaaaatct gtcatcgatc atttgcatat cgtgggtgat atttttgatc
    68101 gaggaactca agcagccaaa gtaatggatc gaatcatgga tttttcctct gtagatatcc
    68161 agtgggggaa tcatgatatt ttgtggattg gtgcgtattg cggctctgag gcatgtttat
    68221 tgacgctttt acggattgcc gcacgctaca actatttatt tgaattagag aaagaatatg
    68281 gattaaattt acgtccatta ttttcttttg cccatcaaac ctatcagaag aatccagtat
    68341 ttacccctaa aaacaggaaa gatctatctg aaagagagca agaagaatta gaaaagatcc
    68401 atcaagcact ttctatcctc caattcaaat cagaacatca gttgttgaat cgacgacctg
    68461 aatttaaaat ggacgatcgt aaattgttgg aaaaaatcaa ctatgaggaa tcaacgatcg
    68521 ttttagaagg tcagctctat gagttgaaag ggacttgttt tcaaacgatc cagccagatg
    68581 atcctaaaaa attcttggaa gaggaacaaa aagtgatcga tagtttgatg tattcattcc
    68641 agcactcgtt aagaatgcaa aaacacatga cgtttttgat cgaaaaaggg ggaatgtatc
    68701 tcacgaacaa tcagcatgta ctgttccatg gctgtatacc tgtagatgaa aaggggcagc
    68761 tcttagcatt tgaattagac caatcttatc gcggaaaaga actgctggga ttttttgaaa
    68821 aacagataac tgaaagcgca aaagatttga ctgcaaatga agatttggcg accgatttga
    68881 tttggtatgc gtggagcggg ccattttcgc ctttattcgg caagagtaaa atggcaactt
    68941 ttgagcgcta ttttctcaca gagcctcata cgcacgtcga aaaaagcaat ccctattatc
    69001 acttgcgaga tgaagagtgg ttcagtgaaa aaattttggc agaattcgat gcaaaagaag
    69061 aaggttcagc aatcatcaat ggacacacgc cagtaaaagt aaaaaaagga gagtcaccga
    69121 tcaaagcaaa cggaaaagca tttgttattg acggaggtct atccaaagct tatcaaaaaa
    69181 caaccgggat tgctggctac tcattactat gcaattctta cggtttccaa atcgtgacac
    69241 attatccgtt cttgcctatt gaacaacaat ttgcaaaaaa atctgatcaa acgaatgtaa
    69301 aaaaagtgat cgagcagcag ctcccacgca aacttaatcg gaatacgaca aaaggaatgg
    69361 aattacagca acagattact caactccaaa ggctgttgga ttatcgaaag gacgattgag
    69421 aaaaatgcta gccaacattg ctcaatagac taaaaagcga ctgcaacaaa aggcgtgtcg
    69481 cagtctcctt cggaattagc tcaaaaaacg gtaaaatatt agaagctatt tttctgttta
    69541 tttctctaat actcaggtca gggcgctgat ttcacgacct cttccttaaa cggtgttctc
    69601 ctgccaactc ctcggtcaca agtcacaata tccaacaaac atgagaagct gttttttgaa
    69661 attgctcctt gtgactcaga gttgaacggc aggctcacga cctaatcaga aaaacgatta
    69721 aaattcattc acaaagaaat cgaataaacg ctgttcagat gaatctacac gatgagaaag
    69781 ttccggatgc cattggacac caaggatttt gtgcatatta tctttacttt ggactgcttc
    69841 aatgagtcca tcgttggaaa aggcgatggc ttttaacaaa ggagaaagat ctttgactgc
    69901 ctgatgatga taggaattca caaagtatga atcagggaga aggtcactta ataaactgtc
    69961 cgaaacgatt ttgacttcgt gagttgcaaa ctgcggggca gtgggctgtt gttcgtgttt
    70021 tacggaccat tttggatata atgaaagatc ttgataaagt gttccctcca acacgacatt
    70081 caatagttga agtcctcggc agactgcaaa aatcggtttt ctttgtttca atgcttccaa
    70141 gatcaatgcc tgttcgaatt ggtctcgttg aatatttgtt tccgtcagtt ttggatgagg
    70201 gtcttccatg taaagttgcg gactaacatc ctgaccgcct gcaagaatga gtttgtcgat
    70261 tttctcaatt agttgaggcg ctgaatctgg agacatgact ggcagaataa gaggtaatcc
    70321 ttgagcatct aaaactgcat ccacaaatcc ctgcggtgta tagcttactt gatttccttg
    70381 gaaagtatct gtcgctcgaa tcagttgatt cccggcaatg ccgatgattg gtttcatttt
    70441 tattccccct aaagtagact aattgtaaca aggatttaaa aaaatttaaa ctatgtaaga
    70501 cttgccccta tacttatctg ttcttgctgt gttactatct gtaaagagaa ttttcgtgtg
    70561 aaattcatga cgatagttga aaatattgct taatttgtta taataaaaag taagcagaaa
    70621 taaaaaggtt gggcgtaagc ttcaaggatc tagttcttca aggggtgaga aggacatttt
    70681 attgaagggg cctgcctata ctaagttatc tttagaggta catactttca atgaaaaaag
    70741 aaagcatgta cctctttttt aaatcgaaag gaagtgaagc aagtgttaga agctttggaa
    70801 caaatccgaa aagcagaaga agaaaatgag aagcgtgctc gaaccctacg gcagcagttg
    70861 aagacctatg cagaagaaaa agagcaagaa ttggagataa tccaaacgaa cagtcggcaa
    70921 cagctgaaac agttggtaga agaaaaagaa caattcgaaa agcaagaatt agaaaaagaa
    70981 aaaatgaaat tgaaagcaga agcagacaag gtgcaagcaa ctttgaaaga ccaatatcaa
    71041 aagcatcaga gtcaagcaat cggtgcaatc attgaaaggg tgaaagaaac ttatggccgt
    71101 cactaagatg gaaaaagtga ccctcatttc agataaaaga aatcaggaga ggattttaca
    71161 agcagtacaa ggaatccaaa atgttgaaat ccgggatctg tatcaaaata gaacaaacaa
    71221 ccagtgggta gaaaaatatt ttccagatac cacgattatt gataaagaag caaaattaag
    71281 cgctttaggc aatcggctga aagacatcca agaagctatc caattcatac aacaccacgg
    71341 cgatagccgt caaaagaaac tccatttgaa acgtcaagaa ctttccttac aagacttgga
    71401 agcaaattat tccgaagaaa atttcatgaa taaattagaa gaagtccttg atttaaaaga
    71461 aaagtgggaa cagctaactg aaaaacgtga tcgtttagaa gaagaggaag aatggcttct
    71521 tcattggcaa cagctggata ttgctccaag tatgtatgca agtaagacga ccatttttga
    71581 gatggggact gtgagtgcag caaattttga aagtttcaag gaagaactaa gtaagcttga
    71641 tgacgtttat atggaagaaa tagattatac gccaaaacaa gtttatcttt cgtatgttgc
    71701 tttaaaaaaa tcagttgctg ctatagaaga aattgctggg aaatacgggt tcaataaaga
    71761 aacgtatcct tatgaacaaa atcctaaaga aaaattgcaa gaaaccaaac agcaattatc
    71821 cattgttcac gagcagcaaa aaaagataac gacagcgtta ggtcagtgca gcggctatat
    71881 ccaagaattt gaatgggtag aagaagtcac acttgccgtt gcagaacggg aaaaagtaaa
    71941 agaacggttt atccatgcca cttatctgat cgttttgcaa ggttgggtag atgcagaaga
    72001 aaaacagaat ttgatgcatg tgttaaacga aactgtatca gaagaagata tatacgttag
    72061 tttcgaaaca ccaaactcag aagagattca gcaggaagtc cctactaaat tgaaaaacca
    72121 tccgcttatc gaaccttttg aattgctgac tgaaatgtac agtctgccaa aatatgaaga
    72181 agtagatcca acgccttgga tgacaccatt ttatcttgtc ttcttcggga tgatggtcgc
    72241 agatgttggt tacggattgc tgatgctcat tgggacaata ctagctcaaa aactatttgt
    72301 tttaccccgc gggatgaaac gtttcgtaaa attctttgag attctctcgg taccatcgat
    72361 catctgggga ttgatctaca gttcattttt cggacaggca ttaccaaaag aactatttgg
    72421 tatccgcttg cctttcccaa tcctatctac gacggatgac gtcaatacga ttttgatttt
    72481 atccgtgatt ttcggtctga tacagatctt ggttggatta ttcgtggcag caaaggaaaa
    72541 tatcaggagg aaagattact tgagtgctgt cagtgatggt tttgcctggc aagggatcct
    72601 gattgggatc gttattgcat tgattggtgt catgttgata aaaaatacag catttgttta
    72661 tcttggcgcg gggctatcga tcattgctgc tttatgtatc ttaatcgtgc cgattatcca
    72721 atcgccgtct aaagtcaagg gtgctgccaa aggagcgtat aatctttacg gattgactgg
    72781 ttatatcggt gatttagtca gttatacacg tttgatggca ctaggtattt caggcggaag
    72841 tattggcgcg gcattcaata tgctagttgc tttcatgccg ccagccgctc gattcagtgt
    72901 agggatcctt ttgatcatcg ctttacacgc actgaatatg tttttgacac tgttaagtgc
    72961 ctatgtccat ggtgcacgat tgcagtatgt cgaatttttt ggaaaatttt atacaggagg
    73021 cggacgtgcc tttgacccgc tgaaaacggc ggaaaaatac gtcaacatca atcataagaa
    73081 aaaaaacaat aaataattgg aggattttta tacaatgatg gattacttaa ttagtcaaaa
    73141 tggtggaatg gtatttgcag tattagcgat ggcaacagcg acgatttttt cagggattgg
    73201 ttcagctaaa ggtgttggaa tgacaggtga ggcagcagca gcgttaacaa caagtcaacc
    73261 cgaaaaattc ggacaggctt tgattctgca attgcttcca ggtacgcaag ggttgtatgg
    73321 attcgttatc gcctttttga tttttatcaa cttaggaaat gatatgtcag ttgtacaagg
    73381 attgaacttt ctaggcgctt ctctgccgat tgcttttaca ggtttgtttt caggtatcgc
    73441 ccaagggaaa gttgcagcag caggtatcca aattttagcg aaaaaacctg aacatgctac
    73501 aaaaggaatc atttttgctg cgatggttga aacatatgcg attttaggtt tcgttatttc
    73561 cttcctatta gtattgaatg cctaagcata aaggaggaaa agcagatgaa cgccattgaa
    73621 aaaatcatct ctcagatgaa tgaagcagca gagcaagaaa gagcggcgtt agagcaagaa
    73681 gagcgaatga agatcgatca aaactttgaa caaaaacgta cgcaaatcga aacagaacat
    73741 caaaaacaaa aagaaaaaca aatcgaatta cttgaaaaaa aataccgtca gcttaggaat
    73801 cgccaacaag tagaagtacg acaagaaaat ttgaatgcga agcaggaatt tttacgacga
    73861 ttgtttgcag atgcaatagc tgaaatggag aattgggatg aatctgaaca aatccagttt
    73921 atcaaaaatt cattgtattc attacctttg acaggcaaag taacattcat tgctggagaa
    73981 aaatcagcag cttatctttc tcagacattg ctggatgaat ggaacaatga acttcccttt
    74041 atgatggttt taagtgatga aactgttgct gatcaagcgg gttttctgat aaacgatcaa
    74101 ggagttcagt ataattttct tttcagcagc cttgtacaag atatccaagg aacgatgagt
    74161 tttgagattg ccaatcaatt attcgaataa ggagggatca gtgtatggaa taccacgaac
    74221 taaatccctt gattcgcgga agagagcttg aacttttgtc caatgagaca tttgaacgga
    74281 tgattcaaac aaattctgtc gaagcacttg gtgaaatatt gaaatccacg atttacagtc
    74341 catatattta tgacggatat gaatatgact tcgaacagaa tcttttaaag gagcagagcc
    74401 gtctatttcg ctggttaaag gaatcagcac cggagcctga aatcgtttgg atctatacga
    74461 tgcgctatac tttccataat ctgaaggttt tgacaaaagc cgagatgaca aatcaaaatc
    74521 tggaccattt gtatatcgac gatggatttt attcattaga aaccttgaag gatgccattc
    74581 atacgcaagt ttcaaatgaa ctccctgaaa gcattatgga cagtatacga gaagtacgtg
    74641 attattttga agagtctacc attcttcagg gaattgacgt gatttatgat cgacagtttt
    74701 tgacagagca gcgacggctt gccgataagc taggatatca agaattgctg acagaagtca
    74761 ttgcttttat tgacttgacc aatatcacaa cgatggctcg aggaatattg cagaaaagaa
    74821 catatgggtt catgacgacg gtactttcca gctcgggaag tatcgaaaaa gaagtatttt
    74881 tgaactttgt agataaagat atggaagatt acacgaattt tctattagag acagattatg
    74941 ctgattttct gcgccctgtc atccgtgagg gaaatcttga tctcgtaaag cttgaacagt
    75001 tgaaagatga ttatctaagt tcattgtatc aagaagcaca aacccaggct ttcggaccac
    75061 tgccactact agcctttttg aacgcaaaag aggtagaaag caaaaacctt cgtttactgg
    75121 ttatcggcaa aagaagccat ttcgaaccag aaacaatcag aaagcgggtg agacaagtct
    75181 atgacgcata agatcggtgt agttggcgac aaagattctg ttttgccatt caaattgttt
    75241 ggctttgatg ttcgttatgg tacaaccaag caaaccgttc gtcaggcaat tgaagaaatg
    75301 gcaaagaatg agtatggcgt gatttatgtc acagagcaat gtgcagcatt gagcccagaa
    75361 acgatcgaac gttacaagga acaaatgata cctgctatcg tattgattcc caatcatcaa
    75421 ggatcattag ggatagggct ggaagaaatt caaaaaaacg tagaaaaagc agtaggtcaa
    75481 aatatcttat agtatgaaaa ggagatagaa attttgcaaa ttggaaaaat cataaaagtc
    75541 tccggacctc ttgtaatggc tgagaacatg tctgatgcca gtatccaaga catgtgtcta
    75601 gtgggagatc taggagttat cggagaaatt atcgagatgc gtggcgatgt ggcgtctatt
    75661 caagtatatg aagagacttc tggaattggt ccaggcgaac cggttcgctc tactggtgaa
    75721 gctttatctg ttgaattagg accagggatc atttcacaaa tgtttgacgg aatccaacgg
    75781 ccattagata cctttatgga aatcacacaa agtaatttct tagggcgcgg agttcaattg
    75841 cccgcattgg accatgagaa aaaatggtgg ttcgaaccaa cagtagaagc cggagaaacg
    75901 gtgagtgctg gagatgttat cgggatcgta gaagaaacaa aagttataaa acataaaatc
    75961 atggtgccaa atgggataaa aggaacgatc aaaacaatcg aaaaaggttc attcactatt
    76021 gatgaaacag tatgtgtcat tgaaaccgaa aatggtgaca aaaatttgac gatgcttcaa
    76081 aaatggccag taagacgtag tcggccaatc aaagaaaaat taaacccgga tgcacccatg
    76141 atcactggac agcgagtgat tgatacattc ttcccagtga caaaaggagg agcagcagct
    76201 gtacctggtc cttttggtgc agggaaaaca gtcgttcagc atcaaattgc aaaatgggcc
    76261 gatgtggaca tggttgtcta tgtgggatgt ggcgagcgtg gaaatgagat gacggatgtt
    76321 ttaaatgaat tccctgaatt gattgaccct aatacaggag aatcgttgat ggaacgtacg
    76381 gtattgattg ctaatacatc aaacatgcca gtagcagctc gagaagcttc tatctatacg
    76441 ggaatcacaa ttgcggaata tttccgtgac atgggttatg acgttgctat catggcagac
    76501 tccacttcac gatgggcaga agcacttcgt gagatgagcg gacgtttgga agaaatgcct
    76561 ggtgatgaag gttatccagc ttatcttggt tcacggcttg cggaatacta tgaacgatct
    76621 ggaagagtca ttgcattagg atcagaaggt cgcgaaggaa gtataaccgc aatcagtgct
    76681 gtatccccgt ctggagggga tatttcggaa ccagtcacac aaaatacatt gcgggtcgta
    76741 aaagtattct ggggtcttga ttccagttta gcccaaaaaa gacattttcc ttcgatcgat
    76801 tggatacaaa gctattctct ttattcaaca gaagtaggga agtatatgga tcatatcctt
    76861 caagctgact gggcatccat ggtcaccgag ggcatgcgta ttttgcagga agaacagcag
    76921 ctgaatgaaa tcgtgcgttt ggtagggatt gattcccttt cagataatga ccgtctgaca
    76981 ttagaagtgg caaaatccat tcgtgaagat tatttgcagc aaaatgcttt tgatgatgtc
    77041 gatacattca cttcaagaga gaaacaattc aaaatgttga agatgatctt gacctttggt
    77101 gaagaaggac gccgtgcgtt gtcgttaggt gcttatttaa gtgagatcat ggacggtacc
    77161 gtatcgattc gtgaaaggat cggacgaagc aaatatatcc cggaaacaga attagaaaaa
    77221 ctagatcaaa ttattggtga aatcaagcaa acgatgcaaa cgatcgtttc agaaggaggc
    77281 atgagcaatg attaaagaat atcgtacgat cggtgaagtc gtcggtccat tgatggcggt
    77341 tgaaaaagtt gctggtgtca aatatgaaga attaatcgaa gtccgtatgc aaaatggcga
    77401 gattcgtcgt ggacaagtat tggaagtcca agaagataaa gcaatggtcc aaatattcga
    77461 aggaacaagt ggtatcaatt tacgtgactc ttctgtacga tttttaggcc atcctttaga
    77521 actaggtgta tcggaagaca tgatcggtcg agtattcgat ggtttgggac gaccgaaaga
    77581 taacggacca gagatcctgc ctgaaaagca tttggatatt aacggagaag taatcaatcc
    77641 agtagctcgg gattatccag atgagtttat ccaaacaggt atctctgcaa tcgatcattt
    77701 gaatacatta gttcgtgggc aaaaattacc agtattttct ggctctggtc ttcctcataa
    77761 agaattagct gctcagatcg cgcgacaagc aacggtactc gattcatcag atgatttcgc
    77821 cgtagtcttt gctgctattg gaatcacttt tgaagaagct gaatttttca tggaagattt
    77881 tcgtcaaacg ggcgcaatcg accgttctgt catgtttatg aatttagcaa acgaccctgc
    77941 aatcgaacgt atcgctactc ctcgtatggc tttgactgct gcagaatatc ttgcttatga
    78001 aaagggaatg catgttttgg ttatcatgac agacatgacc aactatgcag aggcattgcg
    78061 agagatttct gcagcacggc gagaagttcc aggaagaaga ggatatcctg ggtatcttta
    78121 cacgaactta gctactttat tcgaacgagc aggaagaatc cgcggactaa aaggatcggt
    78181 tactcaaatt ccgattctaa caatgccaga agacgataaa actcatccaa tccctgactt
    78241 gactggttat attacagaag gacagatcat cttgactaga gagctttaca agagtgggat
    78301 tcagccacca attgatgtac tcccgtctct ttcgcggtta aaagataagg gaactggacc
    78361 cggaaagaca agagaagacc atgcagctac gatgaatcag ctttttgcag cctatgcaca
    78421 aggaaaacag gcaaaagaat tagccgtagt tttaggtgaa tctgctttat ctgatgtaga
    78481 caagatctat gcgaagtttg ctgagcggtt tgaaaaagaa tatgtcaatc aaggattcta
    78541 cacgaaccgt tcgattactg aaacacttga tctagggtgg gaactgctat cgatgttgcc
    78601 aagaaccgaa ttaaaacgaa tcaaagacga catgctggac aaatatctgc cagaagggaa
    78661 gtgagttctg atggcacgat tgaatgtgaa tccgacccgc atggaattga ctcgattgaa
    78721 aaaacagttg acaactgcca caagagggca taaattatta aaagataagc aagatgaact
    78781 gatgcgtcaa ttcatcctgt tgattcgaaa gaacaaccag ctgcgtcaag aaatggaaga
    78841 gtctatgaca gaagcaatgt ctgattttgt attggcaaat gcttcgatca cggaaccttt
    78901 catcgaagaa ttatttgttt tgccggcgga aaacgttgat ttgtctatcg tagacaaaaa
    78961 tatcatgagt gtaaaagtac ctgtgatgaa ttttgaatat gatgaacagc tgacagacac
    79021 acctttggaa tacgggtatt tgaattccag tgccgaactg gatcggtcaa tcgatcggtt
    79081 tactaccctg cttccaaaac ttttggaact gacagaaatc gaaaaaactt gtcaactgat
    79141 ggcgagtgaa atcgaaaaga cacgtcggcg agtcaatgca ttagaatata tgacgatccc
    79201 tcaattagaa gaaacgatct attatattcg tatgaagctg gaagaaaacg agcgcgcaga
    79261 agtaacaagg ttgatcaaag taaaaaatat gggtaccact gactaagtgg tatctttttt
    79321 taaaagagga aaacgatgat taaacaaagc aaacaaataa agaaacgttt gccggctgtt
    79381 cagcttctcg cattaggatt ttttctcctg attttagttg gcggcggcct acttacccta
    79441 ccatttttta gtaatagtgg gcatgggaca aattttatcg atgcgctttt cactgcaact
    79501 tctgctgtct gtgtaacggg attgacaaca ttgaatacag cagagcattg gaacagtgct
    79561 ggtcaatttt tgatcatgct tttgattgag atcggcggtc ttggttttat gatggttccg
    79621 attctatgct ttgcattgat aaagaagaaa gtcagtctca gcacacgtat tatattaaaa
    79681 gaagcgctga atctagaaga agtatcaggt gtgatgaagc tcatgcttta catcctaaaa
    79741 ttggcctttg ttatccaatt gattggtacg gttgctttat cttttgtatt tgtgcctgaa
    79801 tttggctggt tgaaaggtat ctggtacagt ttgttccatg caatctctag tttttgtaat
    79861 gcagggtttg atttgttagg agacagttta gccaatcacc agcaaaatgt ttatttgatc
    79921 atggtggtat ctgctttgat catttctggt gggttaggct tcatcgtatg gcgtgattta
    79981 ctggaatatc atcagaaaaa gaagatgacg attcattcaa aaatcgcatt aaccgttaca
    80041 ggtacccttt tattaggcgg attcgttgtg ttctttttca cagagcgcaa tggggcaacg
    80101 ctagtggaag ggaatatgtt tcaacgattg gcaaacacct tttttatgag tgtgacacct
    80161 agaacggctg gatattattc agttgattac ctccatatga gccatgcagg tctgatttta
    80221 acgatgtttt tgatgtacat cggcggaaca tctgggtcga cggccggcgg attgaaaaca
    80281 actacgttag gtgtgttgct gatccagatg cgttcgatgt tgaaaggacg tacgagagcg
    80341 gaagcatttg gccgtaccat ccgacaaggc gcagtgttgc gggcacttac tttatttttt
    80401 gttacgttga cgttgtgtgt agtcgccatc atgattttat cggtgactga aacgatacct
    80461 aaaacttctg gcatcgaata tattgctttt gaggtcttct ctgcgtttgg tacagtaggt
    80521 cttacgatgg gattgacgcc agacttgact gcaataggga aattgatcat tatagcgctt
    80581 atgtacattg gacgagttgg tgtattgact gtggtcttct ctctgatgac aaaagcacaa
    80641 agaacacaat caaaatacaa atatccagaa gaaagtgtca tgattggata atttcatctt
    80701 ctgacagaaa cctagtgatt tagtaaaata agaaaataaa ataatttttc ttgcttcttg
    80761 gatactttaa gtgcaaaatg ttcgtttatt ttttgaggca ctacccgttg ttgaacctcg
    80821 ggtagtgcct cttttttatt gaaaggaatc aataagattt gattccttta tttatcaata
    80881 ggttataatt catatttttt tattttttct tttacgatac ttagtccaga agctaaaatt
    80941 tcaaggttat tagaggatag ggaaattcga acgaatgcat ctggttgcgc acgtgtagta
    81001 aagcgtctgg aatgataaag tcggataccg ttagcaagaa agtcattctc aattatctta
    81061 gggtcaatct caggatcaac tggaagaatt cgatagtaag ggacacgatt cgttggtttt
    81121 tctactttga aaagagaatc aaaaagctga ttggctcttt ttgtaagact taatttagtt
    81181 tctaagatcc tatttgcttc atttgaatgg aggatttgag tgatgacttc cacatctaat
    81241 ccagaagttt tcacatttat attaaaaatt gcttgttcta tcttgctttg gacatgtact
    81301 ggaactacaa gataagcaac tcgcaatcca gaacaaataa attttgtcat tccagctaag
    81361 tagattgttt ggttaggtaa taaattttgg aaaggaggaa tgatcgtatg ttgatagtaa
    81421 gttgttaaaa atgaatggat atcatcttca ataacccata gaccttcttt ttcaatcact
    81481 tcggctaatt gctctcgtct tttgtgagag atttgaaacc ctataggatt attacaagaa
    81541 ggcataagaa agacaccatg aatcttcttt tttctacatt cctgaatcaa tatatcaggt
    81601 cgcattcctt gctcgtcaaa atcaattgga acgatctcta aatgatataa ctgagccaat
    81661 tcaatgaaat tagcgtatgt atatcggtct gttgctattc tatcacctgg agagaaaagt
    81721 gcagaaagtg tgatagccaa tccattttga acaccggaaa caatagatag agattctaag
    81781 gacggaatga ctccttgagt ttttaaccaa tttgctgcag tagtcaattg aaaatttgtg
    81841 cctttgggat cttggtaatt aagcaaacca acaagatttg ggttattact agttgattga
    81901 ataaacggga caacgaattt attacattct tcaaaagaac taaccagacc aaaatcaatg
    81961 atatgttcgg ttacttcatt ggtagagatg gtgatggact caaagacatt gggagaaata
    82021 aatgtgccac ttccaatatt cgtatacagc aaacctttct caattccata tttataagcc
    82081 ttgccaacgg tagtaaaatt taaatctaaa taatcagcta gctctctttg ggagggtaac
    82141 tgagtgtttt tctgtaattt attctttaaa atatcttgtt ctaattgttg aatcaacgat
    82201 cgataataag gacgcttcaa tttttgtttg tctggtcgcc aagttaacgg gtaattgtca
    82261 taagaattaa taggcatttt ctccactcct taaaattgta taccatacaa ttttaccatt
    82321 gattgtattg attttagaag ttaatctttt gtgaggaggg aattgtctat gaaaattatt
    82381 aattttgagg aagtagtagc ttcttttagt tttgaagaag cgattcaagt aatgaaaaaa
    82441 tgtttttttg attatgaaag tggtaaaatc agccaaaatc ctcgattggt ggaaatttta
    82501 cctgacggaa aaggcaaaaa tatgtttgcg atgatgcctg cttatttggg ggagaacaga
    82561 tattttggca caaaagtcat taccgctttt ccggataatc atcagtatca tttaccttct
    82621 catttaggag agattttctt gtttgatgct caaaacggac tacccaaagc attgataaac
    82681 gcaaatgcag tcacctggat tcgaacagct gctgtatctg cgctggcaac agattactta
    82741 gcaaatccgc atgcagaaac acttgcttta attggagctg gtcaacaagc attgtcgcat
    82801 ttgactgcga tttctaccat tcgtcccatc actacagtgt ttgtttacga tattttagaa
    82861 gaaagacgaa atacttttat cgctaaagct aaaaaacagt atcctaaaat aacgtttata
    82921 aatgcaatat cagtagaaga tgcagtacgt ccagcggaaa tcatttgtac gttgacctct
    82981 agtaaagagg catttcttca aaaagagtgg gtaagggaag acgcacatat caatgctgtg
    83041 ggcacattta ctcctgatac aagagagata agtacatcgt tgatggaaac ttctcaaatc
    83101 tatgtagatg attactcagc tgccttaaca gaaagcgggg acctgcttat agccatggca
    83161 gaaggagatt tagaggaatc ttgtattgtt ggtacattag gcgaattagt ttctgagaaa
    83221 aaaacatttt ataaaggaga atcgaggccg acgattttcg acgcagttgg tctagctatt
    83281 gaagatcttt gttgtgcaga atatatttat aacaaattag agaaaggggc tttaaaatga
    83341 acttagatta ttacgtagtt gatgcatttg cagatgaggt gttcaaagga aatccagcag
    83401 cggtttatgt gctagaagaa tggctgccag aaggtacgat gcaaaaaatt gccattgaaa
    83461 acaatttatc tgaaacagca tttacagtga agaaaaacca agaatttgag cttagatggt
    83521 ttacgccaga tcgagaaatc gatctttgtg gtcacgcgac attagcaaca gcatttgttt
    83581 tatttaacta ttacaaaata cctgatgaaa cgattaaatt ttctactcaa agcggtaatc
    83641 tatttgtgac aagaattaaa gattattatt acatggattt tcctagtatt atgccgaaaa
    83701 aagtgccgat attgtctgaa tatgaagaag cgattggggc taagattaaa gaagcctact
    83761 tagcacgtga tttatttttc gtattagaag atgaaaaaac ggtggctaaa ttgcagccag
    83821 attttacagc tatcaaaaat tttgaattag gaataggagt catcgtgaca gcagaaagtg
    83881 tgcaaaaaga ctttgtttct agaaccttct ttccaaaatt aacaatcaat gaagatccag
    83941 tatgtggatc agcacattct aatcttattc cttattgggc agaaagaatg aataaaacaa
    84001 aattgtcagc ttatcaaatg tcatcaaggg gcggggatct agaatgtgaa ttaaaagaag
    84061 atcgtgtgat aataggagga aaagcagttt tgttttctaa aggtacctcg tttatttgat
    84121 ttctttcaaa tgaattaaaa ataaattggt ggagagtggc aaccttatag ctacaatggt
    84181 tgttaaaaaa agacgcaaac tttttcaatt taatgaaaag tttgcgtctc attttaatta
    84241 ttggtgtttg ttttttattc aatcaagagt aaaatgcccc atactaacta tcctcggatg
    84301 acttcgattt tataaccatc agggtcaatg acaaagtagt aagaaggcgc agtgccaggt
    84361 aatcctttta agtcagtaat ctcaagaccg gcagctttat gttgctcgtg taatttttct
    84421 agatcatcag ctgctatagc gatatgacca tagccgtctc ctagatcata cgcaccatga
    84481 tcatagttgt aagtcaactc taattcatag tcatccccag gaagtgtcaa ataaacaagt
    84541 gtaaatttgt tttctggaaa atcacggcgg cggctttctt caaaaccaaa tgccttttga
    84601 taaaattcga ttgatgcttc aagatctttc actcgaacac atgtgtgagc cattttcata
    84661 gattcatctc tccttttcct tttactcaat cataacaaaa agaagtaagg aaatagttga
    84721 tttgtgcttg ttcatgagaa atttctcatg taaaatggat agaaaataaa actgttaagg
    84781 tggaaactat tcatggataa accaattttt ggtgaaaaaa agcaatttgc tgattatcaa
    84841 acccgttatg cagcatatat tgtcatagaa agagatcagc agattgcagt aatcgaggct
    84901 ccaaatggtg ctttttttct tcctggcggg gaaattgaag gagaagagac gaaagaagaa
    84961 gcgattgtcc gtgagctgat agaagaaatg ggaattgtgg cagaaatttc ttcttatctg
    85021 ggagaagctg atgagtattt ttactcgaat tatcgtgcaa cttattatta taatccagga
    85081 tatttctttg ttgctgattc ttggaagcgt ataggagaac caactgaaaa gaccaataaa
    85141 atttggtggg tgagccctca agaagcgctt gtgaaattga aacgtggtag ccaccgatgg
    85201 gcagtcgaga gatggatcga aaaaaataaa aactgaaaga aagcgagtag atcagctttc
    85261 tctcagtttt tttatttatt acagtacata ttttttgatt gcttcagcta caccgtgctc
    85321 gtcgtttgat gaagtgatga catttgctgc ttctttgaca agagaaacgg catttcccat
    85381 tgctactcct agaccagcat attcaatcat ggataagtcg ttctcgttat ctccgatagc
    85441 catgatttca tcttgatcaa taccaaggtg ctgtgcgaga ttcgcaactg ccgcaccttt
    85501 acttgcttct ttatttagta cttcgaagta atagggcgca cttttgaccg ttgtgtattt
    85561 atcacggaaa gtttgcggta aacgcgcaat tgctgcatct aaaatttctg gttcgtcgat
    85621 catcatcatt ttgacgatgt tcatgtctgg tgtcatttct tcaggtgttc gatattttag
    85681 cggcatcttg actaaacttg cttcgtggat cgtataagcg ctgatatctc gattaggcgt
    85741 gtagatcgct ttgtcatcga tcgaatggag atgactgccg acacggcgtg ccatcacttc
    85801 gatctctagg aaatcttcat gtgtcagtcc gtgacgggcg atgattttac ctgatttcgt
    85861 ttgttgtacg agtgccccgt tataagtaat aacgtaatca ttatcttgaa aaaggttcaa
    85921 ctcgtttaac tgttcctgga cacctggtaa gggacggcca gtgcataaaa cgatcgaaac
    85981 accttttgca acagcagcgg caatcgcttc ttttacttct tttgttactt ctcttttctc
    86041 atttaataaa gtaccatcga tgtcgatagc gactagtttg atagacataa tttcctccaa
    86101 tatattttaa tttatttcaa tcaatgcgcc attacggata tggctggcaa attcttgata
    86161 agtttcgtca aataaatcat aatgatcacg caaggacgga tcaaccattt cttttggaaa
    86221 gaaaaatcgc tcatctcctt gttcttgtcc cgctaatgca gcaacaagct gactaacctg
    86281 tgataattct atcaaagttc cgtcatttcg caaaatctcg atttgtgtgc gatgtctgcc
    86341 ttgttctggg cgataaaaat cataaggtag atcgtagcta gaattcacag cagtgtaata
    86401 aacaggatta tatccgacct tttctactaa caaagtgagt tcttggacta attttgaatc
    86461 ccggttgcca gaaaaagtag cagatttcag tggtttacga ttcaaaaaac gtttagctaa
    86521 atcatttaaa ataggatcag cagattcaga ccattgagta aagtaagtgc ccaagacccc
    86581 atcatcaagt ttgaggtatt cttctaaagt aaagcttccg tcaagaaatg gcagaagtaa
    86641 ttgagaatgg agttcaaaac tgctggcatc ttcttgatac aactcgtgtg cgcgatgaag
    86701 cagatgctcc aaaattactt ccataccacg ggaaacagga tggaagtaca cttgcacata
    86761 catttgatat cggctaacga tataatcttc cactgcatgc atgccactca tggaaaaagc
    86821 gatcccgcct tcataaggac gaatcacacg taatattcgg gtaagatcaa atgttccata
    86881 ttctgttcca gtgaaataag catcccgaag caaatagtcc attcgatctg catcgatctg
    86941 actagaaatc atttggacaa cttgtgggtt ggggtaagtt ttttggatca cactagctac
    87001 cttttcagga aaaccttctt ctactcggtt aagaatctga tacacttctg tttctggaga
    87061 agtgatgatc tctacagtga tggcttcatg attcgtatgg aagatatgtt caaatgtatg
    87121 agagtaagga ccatgaccga catcatgcaa cagtgccgca cataaagtca ccagccgttc
    87181 ctgatcatcc cagcctccgt tgcctatttt ttcttttgaa aaatttcgag aaaagatatc
    87241 gcagattcgt cttgaaatct cataaacgcc taatgaatga gtaaaacggc tatgttctgc
    87301 tccgtgaaaa gtgaaagaag aagtgcctaa ttgtttaatg cgtcgtagac gttggacttc
    87361 tttcgaattg atcaaatcta agattacttg atgctggaca tggatatagt tatggactgg
    87421 atcacgaaaa actttttcca taggcagcat ttgatattta taaggggttg tcataatgta
    87481 gctcctttat gttattacgt tgttccattc tctggatttg gttgttccat tgctccagct
    87541 ggatttcttt ttttagccag tcggtagaat cctgtaaatc acttgcagaa tacagctttt
    87601 caaaagcttt tgcaaatgct tctttgactt cttttacaaa aaggggttgg tcaagcagtt
    87661 cttcaagtgt tgccattgta gcaggatcca ctgcaggata gccatttgta ccaaagtctt
    87721 cttttaaagc agattgataa aatcgcttca tgacttgccc gcgtttttcc tgattaccgt
    87781 tgacacttaa gtacatcata acagaaatcc catttttgat ccgacgctga gcgataccgg
    87841 caaatttttt gccatggatg cttaaatcat aagttccagg acaataagaa tgggtgattt
    87901 cgaatgcttg tatcggtgcc tttggaaaag cgtatttagt caataggagc atttgctcgt
    87961 aagcgtcatc tatggttatt ttttttgtgt cactttgggg gaaaatcaaa gaaatattca
    88021 aaattccttc atctgatacg atccctaatc cacctgaatt tctgatcact ggagcgtaac
    88081 ctgcatcttt cagtgtttgg atcccgtcac ttagaaaagg agttcgaaca tccttcatac
    88141 ctaagatgac tgtgcagtca tattgccaga aatgtaaaaa gatttgctgg tgttctttag
    88201 aatattccgt taatatatct gttaatgcaa aaggggaaaa gtcgtttttt tgataaatgt
    88261 gctgatctag tcgaatcgca gggttcattc aaccggctcc tttgtataaa ttcatatttt
    88321 ctacaatatt atactctaaa atcaaagagt tgttttggaa aaaaggtttt ctgtaaaaat
    88381 ggacaagttg gttgacaaaa caaaagaaac taggaacaat gtagctatga gaagtaaagg
    88441 agaatgaata atggaattat cacaaggaac accgatttca atcaaattgc gtacaaaagt
    88501 aaaacagcaa ggggaagtgc aagattttta ttttgatttg aaagggcaaa tggtgacaat
    88561 tggcgatacg ctgtacatac gttataaaga gattcaggag gagacgggcg atgagattcc
    88621 tgtaacgatc aaattagttc cagatggtca tgtacagctg atccgtgcag gagaaatgcg
    88681 gatgcgtttg aaatttggct ataaagaacg gttagaaaca tcctatcgaa caccatacgg
    88741 tatgatccag attgctactt ttacgaaaga actgcatgtt agtttgaaag accgtcccac
    88801 aggagggaag gttcgaatcg attatgatct gttcatggga ccggaaaaaa tcggcgaata
    88861 ttatttgacc ttggatttta cagcgtaaat ataaaagaaa agtttgattt tttgtgtctg
    88921 attttgtatg ataaggagta gactgtgaaa ggacgtgtcc tgattggaaa ttagtgtatt
    88981 tgaaggtgca aacaaaagcg aattatcaat gatcgaagta gcacacgcga tcttagaaca
    89041 acgtggcgat gtcatggatt tttctgattt agctaaccag atccaaaact atcttgggaa
    89101 atcagacagt gaaattcgtg attcactcgc acaattttat actgacttga atattgacgg
    89161 cagctttatc tcattagggg ataatcgttg gggcttgcgt tcatggtatg ctatcgattc
    89221 gatcgatgaa gaagtaaacc atggaatgga cgaagaggat gaagatactc cacgtcgccg
    89281 taaacgcaag aaagtcaacg cctttatcaa tgatgatgaa gacgcgatcg attacaatga
    89341 cgatgaccca gaagatacag atctgacaga agaagatgac gatgatctgt ttgatgacga
    89401 tgatgatgaa gatgaagaaa tcgctgctta taattctgac ttgcaagaaa tcggagcaga
    89461 tgacccgaca gatgatgaag aagacttgcc aggaaatatc gaagaagatt taagtatcat
    89521 agacgaagac gatgatgatg aagattatga agaggaagaa ttttctgact ctgaaaaaga
    89581 ataaacatta ttttcttacg agaggttgtg acagagacga tcagcctatc agaataagga
    89641 ggtgtgacac gat
//
LOCUS       DARBET010000004        81761 bp    DNA     linear   BCT 07-NOV-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4027
            SAMEA114569841-rid20112183.denovo.004, whole genome shotgun
            sequence.
ACCESSION   DARBET010000004 DARBET010000000
VERSION     DARBET010000004.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA114569841
            Sequence Read Archive: ERR12203666
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 81761)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 81761)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001972618.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001972618.1
            Genome Coverage       :: 132x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/07/2023 13:18:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,041
            CDSs (total)                      :: 2,975
            Genes (coding)                    :: 2,908
            CDSs (with protein)               :: 2,908
            Genes (RNA)                       :: 66
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 67
            CDSs (without protein)            :: 67
            Pseudo Genes (ambiguous residues) :: 0 of 67
            Pseudo Genes (frameshifted)       :: 26 of 67
            Pseudo Genes (incomplete)         :: 28 of 67
            Pseudo Genes (internal stop)      :: 20 of 67
            Pseudo Genes (multiple problems)  :: 6 of 67
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..81761
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA114569841-rid20112183.denovo.004"
                     /isolate="VRE4027"
                     /db_xref="taxon:1352"
                     /country="Denmark"
     gene            7..1005
                     /locus_tag="SCJ86_000328"
     CDS             7..1005
                     /locus_tag="SCJ86_000328"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321168.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEG1167918.1"
                     /translation="MGETGWRATTLNYQWPVAFSLLTFYPFFQLLRGEEINRKIYWVS
                     IPLLIFLTNQEQVNACFFVLTSIVSLYLIVNGRYNYKLSVFSIISLAELIFSLTTPGN
                     ALRAAHEINKWFPEYKNFNFLNKLDLGISSFGKPFFLDMNILFLLLFFLIFLLTYRKC
                     QNYYVRILTALPFFLNLIIYFGNTMGQSFTYVNGNKRAMIWSSSNLNNLFTELGTKLS
                     LFYPGTWIATLVVLALLLCLIVGIYLSFDNKKTSIFLVILMIMGFCSRLIMGFSPTVW
                     ASGMRTYYILYVVIAILVLMAVKELMKSMSVQKNEFMQFGLTVLGICTFIITVINR"
     gene            complement(1245..1328)
                     /locus_tag="SCJ86_000329"
     tRNA            complement(1245..1328)
                     /locus_tag="SCJ86_000329"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1292..1294),aa:Leu,seq:gag)
     gene            1383..1562
                     /locus_tag="SCJ86_000330"
     CDS             1383..1562
                     /locus_tag="SCJ86_000330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303756.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEG1167919.1"
                     /translation="MGGLIKMEKYDGEFSGLGMILGVLIGLAFGRFLLGFMLGIICGI
                     AMDWAANLWNDYHDN"
     gene            1711..2928
                     /locus_tag="SCJ86_000331"
     CDS             1711..2928
                     /locus_tag="SCJ86_000331"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002357817.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal phosphate-dependent aminotransferase"
                     /protein_id="HEG1167920.1"
                     /translation="MREFEKSNKLEGVSYDVRGPVLEEAERMQEEGISILKLNTGNPA
                     PFGFEAPNEVIRDLIMNARDSEGYSDSKGIFSARKAIEQYCQLKHFPNVTINDIYTGN
                     GVSELITMCMQGLLDNGDEVLVPMPDYPLWTASIALAGGTPVHYICDEEAEWYPDIDD
                     IKSKITSRTKAIVIINPNNPTGALYPKELLEEIVEVARQNNLIIYSDEIYDRLVMDGL
                     EHIPIATLAPDLFVVTLNGLSKSHRVAGFRVGWMVLSGDKSHVKGYIEGLNMLSSMRL
                     CSNVLSQQIIQTALGGYQSVDELLLPGGRIYEQREYIYNAINDIPGLSAVKPKAAFYI
                     FPKIDTKRFNILDDEKFVLDFLHEHHILLVHGGGFNWNQPDHFRIVYLPKMDDLKQTA
                     KKMREFLATYRQK"
     gene            3112..3948
                     /locus_tag="SCJ86_000332"
     CDS             3112..3948
                     /locus_tag="SCJ86_000332"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339389.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HEG1167921.1"
                     /translation="METLIESFGTIIKEIRKEQKMTQKMLSHDICSQSVLSRIENNEE
                     LPNVLVMMQICQRLGVTIDHVMNLSRKTTRTNDKLFELLDSCFQKRDYRKLEQVLKSS
                     DISENLYQATDFQRYYYYLGVCEFTLRQNISQSLYYLKEALSYSSQKERIHVSDTEIQ
                     LISCIGKIYGVAGKTAEARYYLSRSIQLLHQSADERSKSEFSQIFYNYGNFLFHQNEI
                     DEALEQVNQGIYWAQEKNSYYYLNDLFVLKSLIYKRKEEPERAAFYEGLAQAVKQISK
                     NL"
     gene            4024..5172
                     /gene="nagA"
                     /locus_tag="SCJ86_000333"
     CDS             4024..5172
                     /gene="nagA"
                     /locus_tag="SCJ86_000333"
                     /EC_number="3.5.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016176784.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylglucosamine-6-phosphate deacetylase"
                     /protein_id="HEG1167922.1"
                     /translation="MRTFIYADKFFLASGVKHAGYLEITDGLFGEYTKELPEGEVNII
                     DQSGKWIAPGLVDTHIHGYMNHDVMDNDAEGIKVMSEGLLSCGVTSFLPTTLTSSKER
                     LKDVAETIGKMYQEAPGAKIKGIYFEGPFFTEEHKGAQNPSYFGDPDLDTFHEWQEAS
                     GGLIKKIALAPERQGVEEFVKQVTEEGVVVSLGHSNATLEEAQKAVEAGASVFVHAFN
                     GMRGLNHREPGMVGALLSLEHVFSELICDGHHVHPNAAGILMEKAGHDHVALITDCMM
                     AGGMPDGNYNLGEFPVVVKEGTARLESGNLAGSILKLKEAIKNVVDWGLATPEQAVMM
                     ASLIPAISCKIDDTCGKIAQGRDADFIVLNPDMTLEATYLDGVERFHA"
     gene            5392..6450
                     /locus_tag="SCJ86_000334"
     CDS             5392..6450
                     /locus_tag="SCJ86_000334"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318398.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AEC family transporter"
                     /protein_id="HEG1167923.1"
                     /translation="MNLFDIFRSTLTDMSIVSAITSTVFIILLGFVCRKKGIFSESVG
                     KILSKVVLTVALPALAFRAFMQDINTELLMQGINVLIWGLVIYIILILVSKPFFLSYK
                     GDKQTTLRVLTIFGSTTFFGTPIVSAIYGPVGVMYSSIFNIGYRIFLYSYGYIKMSGL
                     KMEAKNIKAMFVNPIIIATFAGLFIWLFQGYLPQVSVTGAEGEISQVAFLRIDQTAPW
                     LFQPMDYLAGLSSPLAWLAIGATLGEVSFKKAASEKTSWYYSLVKVVFVPALNIVILA
                     VLTATNILPVNFEALATIVIMMATPAATVAAAYAISFDREALLASNASLLSTIFAVVL
                     TPVWIVVLQMIHGIGLFS"
     gene            6454..7488
                     /locus_tag="SCJ86_000335"
     CDS             6454..7488
                     /locus_tag="SCJ86_000335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375422.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lactate dehydrogenase"
                     /protein_id="HEG1167924.1"
                     /translation="MEEESMRIACYGVRPNEIDFFEKLNIYHYDLSLYEELLTHDNIV
                     SAKDHDAVLLRGNCIADETNLAKMQEYGIRYVFTRTVGVNHIDLQAAADFGMTVARVP
                     SYSPNAIAELSLTFAMMLLRNTAYTTIRTSFKDFRVDEQMFSREIRNCTVGIIGTGRI
                     GLTEAVLFKGLGAKVIGYDIHQTEAAKKVLTFHSLEELLAKSDIVSIHVPHIPGENDQ
                     MINEGFLAQMKKGSILINTARGELQDNEAILRALKSNHLAGFATDVFANETEVFFRSF
                     KPWETIPDPAIQQLVEMYPRVLVTPHVGSNTDEALSNMISTSFENFREIIETGKTKNE
                     VSLPKARQLR"
     gene            complement(7590..8306)
                     /locus_tag="SCJ86_000336"
     CDS             complement(7590..8306)
                     /locus_tag="SCJ86_000336"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375421.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HEG1167925.1"
                     /translation="MMTNVLIIEDDPMVAELNRTFVESIPNMNVIESFRNVEEGKKYF
                     RQKNADLLLLDVYMPGESGIHFLSWLRQQGNDIPVILITAADDIETIKNVIRYGALDY
                     LIKPFTLQRFQLAIEKYLAFSQTIQTTEKADQKLIDQVFNHTTPIHEIPSFIDLPKGL
                     SKLTLKKISAFILKEDTIFSTKELAQKSSLSRISVKKYLNFLVEQDILNERIKYLEIG
                     QPLTCYELTKNGRKKLTDYC"
     gene            complement(8275..9060)
                     /locus_tag="SCJ86_000337"
     CDS             complement(8275..9060)
                     /locus_tag="SCJ86_000337"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297127.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Spo0B domain-containing protein"
                     /protein_id="HEG1167926.1"
                     /translation="MYLNGIETISSVTPLFSNDRFLGAVATLRNRSEVMKLMEELGGT
                     KQYINSMRAQTHEFMNKLHVISGLIDLKKYEEVTHFIQQLNQNYKKEIGQVSSLIKVP
                     AIAGFLLGKINEAKEQSVEMLLESDSFIPKMEMDDSIHSLLQVLGNLLDNAKEAVLQK
                     HERNGQVRIHLNYDTEAEVFIVTVCDGGVGISEEISEKIFTLGFSTKGINRGFGLNLI
                     HSIVKRHGGFLEFSKQTNGGTIVYLELPKKEDQNDDKCPDNRR"
     gene            complement(9051..9800)
                     /locus_tag="SCJ86_000338"
     CDS             complement(9051..9800)
                     /locus_tag="SCJ86_000338"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine kinase"
                     /protein_id="HEG1167927.1"
                     /translation="MNRSSVSDKISSIAKIVANDKLIQKKLQTGDPTHAIQGITNEIM
                     IASNLDFIVVMDPEGIRYSHADPSYIGLPFSSHEDVKKVIGGQEFFSEKEGMLGFGFR
                     YFVPIYYDQEIIGAVCVGLTNNTIDNQVKDGQKMIVFSLIIGLSVGIIGAVFLAQKMK
                     KVLLGLEPQEIAEQLSEKTIIENEVDEGILAISSDKTILLINTPAKNIFYQTEQLLDF
                     EVGKKSVIIYMTFCLKRRLSIKRPFGLNPCI"
     gene            10098..10622
                     /locus_tag="SCJ86_000339"
     CDS             10098..10622
                     /locus_tag="SCJ86_000339"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292922.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NfeD family protein"
                     /protein_id="HEG1167928.1"
                     /translation="MIGGIALETIYWYTLLICAGLAVLLIIFGDVFDFDGPLDPMLII
                     PWLTFTSLFGFLGERLTDQSSLFLFIASGGISIVLVFLLNFYILMPMKHAESTMSSSE
                     KTLEGQVAVVITPIPVRGMGEIQLKSVTGSISRPACFYSPQEQTAERGTKVLIIEIKE
                     RICYVMPYESMIKA"
     gene            10638..12137
                     /locus_tag="SCJ86_000340"
     CDS             10638..12137
                     /locus_tag="SCJ86_000340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flotillin family protein"
                     /protein_id="HEG1167929.1"
                     /translation="MEIPVVFIAIAIAALILLMLLIVFVAKYQTAKPDEALIISGSYL
                     GSKNVHIDESNNKIKIVRGGGAFVLPVFQRSNRISLLSSKLDVSTPEVYTEQGVPVMC
                     DGTSIIKIGSSVEEIATAAEQFLGKTREELENEAREVLEGHLRSILGSMTVEEIYQNR
                     DKFSQSVQEVASVDLAKMGLIIVSFTIKEVRDKNGYLDSLGKPRIAQVKRDADIAEAE
                     ALKETRIKKAQAEKESQAAELQRQTEIAESLKEKELKLATYKQEQDVAKAKADQAYNL
                     ESARAQQQVIEQEMQIKIVERQKQIELEEKEITRREKQYDSEVKKKADADRYAKEQEA
                     QAQKVKEVTEAEAERFRVEALAEAEANKTRLAGQAEAEAALAKGKAEAEAKQKIANAF
                     KEYGEAAVLSMVIDMLPQLMREAAQPLGNIEKISVVDTGSSSGETSGANRVTNYATNL
                     LSSTQETLKATTGLDLKEIVENLSTRGTTSQVNMYEAPKEAEQNSMKEE"
     gene            complement(12225..12827)
                     /locus_tag="SCJ86_000341"
     CDS             complement(12225..12827)
                     /locus_tag="SCJ86_000341"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339383.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FUSC family protein"
                     /protein_id="HEG1167930.1"
                     /translation="MQFGRFRLGMRTIKSALAVFLCILFFHLTQRGIPMIAALSAVFS
                     LRQDLTTTVSFGKSRIIGNTLGGTLAIIYFFVKSYFHNDFLVELILLPILVIIVIVVS
                     DGINNNSGIISAIATLLLIALSIPQGESALYAIQRVLDTFIGTFIAIGINFFLRPPET
                     EKKEELSEDLVELQKKERLLRTNLQEIEAKIQKQKKNSED"
     gene            12931..13887
                     /locus_tag="SCJ86_000342"
     CDS             12931..13887
                     /locus_tag="SCJ86_000342"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aromatic acid exporter family protein"
                     /protein_id="HEG1167931.1"
                     /translation="MRIGMRTVKTVISVFFSMVTAGSLSLLYWPAAGIIALLSVGNTK
                     RSTLRTGMNRIAAFILATATALFSFFAVGYTIFGFSLFLFLFIPLASRFKLTEGIVVN
                     SVLVTHYLVEENMSWQLIGNEAGLMAIGLVFALLANVYMPDRTKQLKENQIQIEKEFR
                     NILRQMAEFLLSENKEDVQIKCEHLLTFIRESQEDAREHQENYWLRQPLYYETYFSMR
                     RAQANVIKDMLENLERIQQPAYYGKHIYGLLIYTAETFSESNDGRQILTRIEEVYVLY
                     RQMPLPTSRPEFEDRAELFQFLQSFKSFIEIKAEFSQQKIQK"
     gene            complement(14192..15148)
                     /locus_tag="SCJ86_000343"
     CDS             complement(14192..15148)
                     /locus_tag="SCJ86_000343"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297120.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HEG1167932.1"
                     /translation="MKKYQWGIVGLGTIAHEFAESFNQETSELTAVASRTSEKAENFA
                     HRYNIPKAYGSYQEMLDDAEIDIVYIAVPNRQHIDHILAALEAGKHVLCEKAITMNKK
                     ELADAMRLAEEKNLILAEAMTIFNMPLYQQLRSIMDTGKLGALKMIQAPFGSYKEPDP
                     KNRFFNPELAGGALLDIGTYAVSFARFFLSSQPEVVASTMVPFETGVDEQSVTILRNK
                     ENELAAVSLTFQAKMPKVGIVAFENGYVTIADYPRADRAEILFTDGTKEFIESGNTSE
                     AMNYEIKNMVHMIEGDLPNRSLFLTKDVIEILDQMQVLWKNM"
     gene            15322..15882
                     /locus_tag="SCJ86_000344"
     CDS             15322..15882
                     /locus_tag="SCJ86_000344"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628367.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEG1167933.1"
                     /translation="MHQVYIDLIMSVLLEQFETEKNFLDEYLLISPEQWQKWKDGEKN
                     LPSEAMQKVKNLFTDYEWMLTQKILRQTIIFPEKRNVAVAEYKQIKTRIAQKWLSSKA
                     AKVELIPLKNNQQAGRYLDLKVSIQYDEWGYDDILNFRLPADVQKQIEGEKIELLDWV
                     NENLEETYVHEANGEREEQRKDFSSE"
     gene            15875..16699
                     /locus_tag="SCJ86_000345"
     CDS             15875..16699
                     /locus_tag="SCJ86_000345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M15 family metallopeptidase"
                     /protein_id="HEG1167934.1"
                     /translation="MNKFLGFGAVVIMIAAMGYVIFSDDDSHVQAGNQAVERTSTKTT
                     ASKEKNSQTTSSKVKDKETKDRTSKQLPDVKTDDWKLVLVGPDHKLEKEIDESTQLAS
                     LSNGYLIDKRIAPEYEAFAKAAEAAGFPLVMVSAYRSVSSQQQVFEQNVQDVMSRQGV
                     SEEEATKITKETITEPGYSEHHTGLAVDVVDQNWYNSYPSTVLDASYGDQPGAKWIAD
                     NAAQYGFIVRYPKGHEDITKITYEPWHLRYVGKENAEYITKHQLTLEEYLNELNEK"
     gene            16709..17326
                     /locus_tag="SCJ86_000346"
     CDS             16709..17326
                     /locus_tag="SCJ86_000346"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298743.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 73 protein"
                     /protein_id="HEG1167935.1"
                     /translation="MAKKKWKKQKRKNIRWPAVVAGVAVILLAFVFSLKNLSSPFTDN
                     QQENEAQSHQQFIERLVPHAQELQDGYGVLPSIILGQAILESNWGKSTLASKYNNLFG
                     IKAYGDQKKVSLETKEFVNEEWITIQGDFKVYDSWEQSMDDHTQLFVQGVDWNPALYE
                     KVITATNYQEAAQALQDAGYATDPGYAQKIIQVIETYQLNQYDRR"
     gene            17344..18027
                     /locus_tag="SCJ86_000347"
     CDS             17344..18027
                     /locus_tag="SCJ86_000347"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324126.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrolase"
                     /protein_id="HEG1167936.1"
                     /translation="MAEQFTPEIMTELRKDIVRVPEIILEASGIKIFGKLIRSIIFTT
                     DIAIIRNTNANAVIAVYPFTPHPAITKSIIEAADIPVFSGVGGGLTQGARSSYMSMFA
                     EAQGSLGVVLNGPTPLETVKEVCRVVDIPVISTVTSKYTEIDEKLAAGISIINISAGK
                     ETAEAVRHFRKKYPELPIIATGGPTEESIAETIQAGANAITYTPPSSGELFSRKMEKY
                     RETEKKNHE"
     gene            18710..19945
                     /locus_tag="SCJ86_000348"
                     /pseudo
     CDS             18710..19945
                     /locus_tag="SCJ86_000348"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738728.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter substrate-binding
                     protein"
     gene            19953..20840
                     /locus_tag="SCJ86_000349"
     CDS             19953..20840
                     /locus_tag="SCJ86_000349"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HEG1167937.1"
                     /translation="MKKEKLKRFLDQDNVVGYVFASPFILGFLLITVIPMILSLYYSF
                     TDYRLGNPVSWIGLDNYIKMLSDARFLNSIRVTFLYVFASVPVKLIFGLLIAYLLTRN
                     IKGTTFFRSLYYVPSLIGGSISIALVWRELFSKNGLVNIFLERLGAPALSWFSDQKLV
                     MIPLVLMSAWQFGSAMIIFAAGIKEIPGSYYEAAEIDGANKWQTFYKITLPLLSPIIL
                     YNLVMQTIAAFMTFTQAFIITQGGPNDATNFYALYVYNQAFSFNSMGYASAISWIMLL
                     IMGAVTFLIFKSSKKWVVV"
     gene            20857..21693
                     /locus_tag="SCJ86_000350"
     CDS             20857..21693
                     /locus_tag="SCJ86_000350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327952.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HEG1167938.1"
                     /translation="MNNTVTKWFQYGFILLLGLVMIYPVLWMIAGSFKTDQEIMSGSL
                     NLIPETFQWENYAKGWAGFAGISFFTFFRNSFVISAVSTIGTVLSSTCIAYALSRIDF
                     KGKRFWFVVMLVTMMIPAQVILIPQFIIYNRLNLVGTYVPLILPHFFGQAFFIYQIMQ
                     FMVSIPKELDESAIIDGCSKYSVFTKIIFPLLKPSIITTIIIQFYWKWDDFMGPLIYL
                     NKPRSYTVSIAIKLFADAGSSTTYASMFAMSTLSLLPVFLIFLFFNKYLVQGISTSGL
                     KG"
     gene            21696..22319
                     /locus_tag="SCJ86_000351"
     CDS             21696..22319
                     /locus_tag="SCJ86_000351"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289649.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEG1167939.1"
                     /translation="MKKPIEEMFLYKLNYYVCRIFLTSLISNALFALVLGCLFTLGLV
                     LSARVVYLIYFLMMYPSYYLSVHYLAEKKVCWRKGKIKRMCFIITLFQLFVIIFAVDW
                     YLLVGKIGAWYLIPFLLCLLLFVSHSFFSTLIDICKEDKADSLVYLIKRNIWLLSKNV
                     KGFLWYLCLLYGLFWCIQPDFLLTALLYPGLPFVTVFCLKEKENDFK"
     gene            22316..23608
                     /locus_tag="SCJ86_000352"
     CDS             22316..23608
                     /locus_tag="SCJ86_000352"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738727.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HEG1167940.1"
                     /translation="MKKKYVQVGLGGRARFFYEAIASTYHQTCELVGFCDVSQTRMDY
                     ANRILVNKYGQKKVKTCMPSQFEQLLDDVRPAEVIITSVDRTHHDYIIRAMKKGYDVI
                     CEKPLTIDEEKAQEIITVQKQTGRSLRVTFNYRYAPYHTKIKELIESDVIGDVFSIHF
                     EWLLNTQHGADYFRRWHRNKHNSGGLLVHKSTHHFDLVNFWLNSRPQKVFAFGGLNFY
                     GMHNAEKRGVEIFYSRCYGSELAKADPFAIDLSANEELREMYLEAEKDSGYIRDRSVF
                     SDDISIEDTLGVTVKYETGAILTYSLNAYMPWEGFNVSFNGSKGRIEMKVIEKSYINA
                     GGDKKDEGAAVLQSIHVYPMFGEPYEVIVEQEAGGHGGGDPRLLDDLFGEEKEEDPWN
                     RAATHVDGILSILTGIAANHSIASGKAVVIDELVSLKE"
     regulatory      23967..24064
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00167"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="purine riboswitch; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="guanine and/or adenine"
                     /db_xref="RFAM:RF00167"
     gene            24180..24761
                     /locus_tag="SCJ86_000353"
     CDS             24180..24761
                     /locus_tag="SCJ86_000353"
                     /EC_number="2.4.2.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400819.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="xanthine phosphoribosyltransferase"
                     /protein_id="HEG1167941.1"
                     /translation="MKELVERIQKDGRILGEGVLKVDSFVTHQVDPVLMEQMGKRFAE
                     VFKEQGITKVVTIEASGIAPALFAARELDVPMIFARKAKSLTMDEELLTASVYSFTKQ
                     VTSTISISRKFLSSEDKVLIIDDFLANGQAAKGLIELCQQAGAQVEGIGIVIEKSFQD
                     GRQLLEEMGLRVVSLARIASLTNGQVEFLEEDA"
     gene            24764..26071
                     /locus_tag="SCJ86_000354"
     CDS             24764..26071
                     /locus_tag="SCJ86_000354"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306869.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="purine permease"
                     /protein_id="HEG1167942.1"
                     /translation="MEKETQNGKAAVLGLQHLLAMYAGAVAVPLLIGTGLGFNQEQMT
                     YLISIDIFMCGLATLLQLTVNRFFGIGLPVVLGCAIQAVAPLILIGTNEGVGAIYGSI
                     IASGIFVVLVSGVFSKIKKLFPPIVTGTVITVIGLTLIPVAIEKMGGGNATAADFGDK
                     TNLLLAFVTILLIVGVQMLAKGFIRSIAVLIGLVGGSILAAFMGMIDLSAIGDAPLFH
                     VPQPFYFGKPTFDVWSILLMIIISMVSMVESTGVYFALGDITGKNVGEEELKKGYRAE
                     GLAVILGGIFNTFPYTGFSQNVGLVQLSGIKTRKPIYFSAFFLIVLGLFPKIGALAQI
                     IPEPVLGGGMLVMFGMVAVQGIRMLLEVDFSNDKNLLIVAVSVGFGLGFNVTPTLFSQ
                     MPQTLQMFTGNGIVMSSITAIILNLIFNGLKKDEEILEEAFKK"
     gene            26364..26852
                     /gene="purE"
                     /locus_tag="SCJ86_000355"
     CDS             26364..26852
                     /gene="purE"
                     /locus_tag="SCJ86_000355"
                     /EC_number="4.1.1.21"
                     /EC_number="5.4.99.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318412.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-(carboxyamino)imidazole ribonucleotide mutase"
                     /protein_id="HEG1167943.1"
                     /translation="MTAKISVIMGSTSDWETMKEACQILDEFGVSYEKKVVSAHRTPD
                     LMFEYAQNARKKGIKIIIAGAGGAAHLPGMVASKTTLPVIGVPVQSRTLNGLDSLLSI
                     VQMPGGVPVATTAIGKAGAINAGLLAIQMLSMYDLELEAKLAERRSMLAKTVIESSDQ
                     LG"
     gene            26845..27969
                     /gene="purK"
                     /locus_tag="SCJ86_000356"
     CDS             26845..27969
                     /gene="purK"
                     /locus_tag="SCJ86_000356"
                     /EC_number="6.3.4.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005876455.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-(carboxyamino)imidazole ribonucleotide
                     synthase"
                     /protein_id="HEG1167944.1"
                     /translation="MVKPLLPGSTIGIVGGGQLGRMMAISAKEMGFKVGVLDPVSDCP
                     AAQVADWHIESVYDDTFALKELARRTDVITYEFENVSVEALNAILPMSFIPQGTDLLA
                     ITQDRLLEKSFLETNNIVIAPYATIVSPTDIQDAIDGIGYPCVLKTTRGGYDGKGQYV
                     LKSRADLAPAMDLLREGTCVLEAWIPFEKEISIMVAGNGQGDFTTFPVVENIHHNNIL
                     HETIAPAAIDQDVIEEAERIARVIAEAVSLSGVLGVEMFLTKTGGLYVNELAPRPHNS
                     GHYSIEACSMSQFDAHIRGICGWPLGEKVRLISNAVMVNVLGEQLLESYRLIENKPDW
                     QFHFYGKKQAKKGRKMGHITILTEDIQQTLEEIKQTNIWD"
     gene            27982..29277
                     /gene="purB"
                     /locus_tag="SCJ86_000357"
     CDS             27982..29277
                     /gene="purB"
                     /locus_tag="SCJ86_000357"
                     /EC_number="4.3.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenylosuccinate lyase"
                     /protein_id="HEG1167945.1"
                     /translation="MLERYTRPEMGNIWTEENKYRAWLEVEILADEAWAELGEIPQED
                     VKKIREHASFDIDRILEIEKLTRHDVVAFTRAVSETLGEERKWVHYGLTSTDVVDTAY
                     GYLIKQANDILREDLKRFTEIIGEKAKEHKYTVMMGRTHGVHAEPTTFGLKLATWYSE
                     MKRNIERFEHAAKGVEAGKISGAVGTFANIPPFIEEYVCKQLGIRPQEISTQVLPRDL
                     HAEYFSAMALIATSIERFATEIRGLQKSETREVEEFFAKGQKGSSAMPHKRNPIGSEN
                     MAGLARVVRGHMVTAYENVTLWHERDISHSSAERIILPDTTILLNYMLNRFGNIVKNL
                     TVFPENMKRNMSATFGLIYSQRVMLKLIDKGMTREEAYDLVQPKTAYAWDNQVQFRPL
                     LEEDPTITSVLSKEEIDDAFDYHHHLKNVDVIFKRVGLE"
     gene            complement(29457..29834)
                     /locus_tag="SCJ86_000358"
     CDS             complement(29457..29834)
                     /locus_tag="SCJ86_000358"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315160.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEG1167946.1"
                     /translation="MEDAIKQGQLFLSELTAEERSCLYNDLLSIKDVYLCNKDTSFYT
                     FRKIRRATKRCAECRKRTFLYQTEHKGELSISQIHEQKRFKNHYRIQILFNGQEMEYA
                     FLENLLQHLHNQHFSYNSQAIYA"
     gene            30090..30818
                     /locus_tag="SCJ86_000359"
     CDS             30090..30818
                     /locus_tag="SCJ86_000359"
                     /EC_number="6.3.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374406.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylaminoimidazolesuccinocarboxamide
                     synthase"
                     /protein_id="HEG1167947.1"
                     /translation="MEKKELLYEGKAKKIYLTSDENLIWIEYLDQATALNGARKDQIS
                     GKGELNNQITSAIFRYLTQRGIKNHFIEQLSKTEQLAKKVQIIPLEVVIRNIAAGSFS
                     KRLGVPEGKEFIKPIIEFYYKDDQLDDPFINEEHIYFLQLAKPEEISELKAQALAINE
                     ILTELFAKVGLVLVDFKLEFGRSNGQILLADEVTPDTCRLWDERTNEHMDKDVYRRDL
                     GDLIPVYQEVYNRLSELTEEKERV"
     gene            30818..31072
                     /gene="purS"
                     /locus_tag="SCJ86_000360"
     CDS             30818..31072
                     /gene="purS"
                     /locus_tag="SCJ86_000360"
                     /EC_number="6.3.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339374.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylformylglycinamidine synthase
                     subunit PurS"
                     /protein_id="HEG1167948.1"
                     /translation="MYFVKVYVTYKDSVLDPQGEAVKKAVHRMGYETIEDIRIGKYFE
                     IQLADAEEPIDTVIETICDKLLANVNMETYRYEIKKMEEV"
     gene            31074..31745
                     /gene="purQ"
                     /locus_tag="SCJ86_000361"
     CDS             31074..31745
                     /gene="purQ"
                     /locus_tag="SCJ86_000361"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010737438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylformylglycinamidine synthase
                     subunit PurQ"
                     /protein_id="HEG1167949.1"
                     /translation="MKFAVIVFPGSNCDADMLWAVREIMGADAEFVRHDESSLEGFDG
                     VLLPGGFSYGDYLRCGAIARFSTIMEEVIRFAEEGKPVFGTCNGFQILTEAGLLPGVL
                     RRNESLHFICKTVPLKVNPKTAFTSEYEADEIIYLPVAHGEGNYFCDEETLQELKKNN
                     QIAFTYNDNINGSVENIAGITNKAGNVLGMMPHPERAVEDLLGSEDGKRFFASILKNF
                     GKVTV"
     gene            31746..33968
                     /gene="purL"
                     /locus_tag="SCJ86_000362"
     CDS             31746..33968
                     /gene="purL"
                     /locus_tag="SCJ86_000362"
                     /EC_number="6.3.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylformylglycinamidine synthase
                     subunit PurL"
                     /protein_id="HEG1167950.1"
                     /translation="MKTLKEPTAEEIKKKRIYAEWGLTDEEYRMIEEDILGRMPNYTE
                     TGLFSVMWSEHCSYKNSKPVLRKFPTSGPHVLQGPGEGAGIVDIGDGLAVVFKAESHN
                     HPSAVEPYEGAATGVGGIIRDIFSMGARPIALLDSLRFGELDNPRTKYLLEEVVAGIG
                     GYGNCIGIPTVGGEIAFDPCYEGNPLVNAMCVGLIEHKHIQKGQAAGVGNSIMYVGAK
                     TGRDGIHGATFASEEFNQEEEQQRSAVQVGDPFMEKLLLEACLELILHHSDSLIGIQD
                     MGAAGLVSSSAEMASKAGSGLILTLDEVPQRETGMTPYEMMLSESQERMLICVKSGEE
                     EKIQELFQKYDLDAVTIGKVTDDGQYRLYHQGEEVANLPVDALAEDAPVYHKEMKEPA
                     RIVEFQQLAPYQPVIEEPGQVLLDLLQQPTIASKRSIYETYDSQVQTNTVVRPGSDAA
                     VMRVRGTNKALAMTTDCNARYLYLDPKIGGQIAVAEAARNIIASGGKPLAITDCLNYG
                     SPDKPEVFWELSTSADGIAAACETLGTPVISGNVSLYNETDGQAIYPTPMIGMVGLIE
                     DHKHITTQEFKKSGDLIYILGKTFADFDGSELQKMQLGRIEGVIRNFDLSIEKRNQEL
                     VLTAIQNGLIESAHDCSEGGLAIALAESAFKHQLGISVQFELSSAQLFAETQSRFVLT
                     VAPENKTRFEEMMGDAAVLAGKVTDEAIIEISATDGQIKIETAVARKCWEDAIVCLMK
                     "
     gene            33953..35392
                     /locus_tag="SCJ86_000363"
     CDS             33953..35392
                     /locus_tag="SCJ86_000363"
                     /EC_number="2.4.2.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735680.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidophosphoribosyltransferase"
                     /protein_id="HEG1167951.1"
                     /translation="MSYEVKSLNEECGIFGVWGHPDAASVAYFGLHSLQHRGQEGAGI
                     VSNDRGKLKGHRDLGLLSEVFKDQRKLQQLTGEAAIGHVRYATAGNGSVDNIQPFLFK
                     FHDQSIGLAHNGNLINARSLRKELEENGAIFHSNSDTEILMHLIRRSEETDFLNKLKS
                     ALNEVKGGFAYLLMTETAMIAALDPNGFRPLAIGQMKNGAYVIASETCALEVIGARFI
                     RDVQPGEIVIINDEGIQIDCFTKDTQLSICSMEYIYFARPDSNIAGINVHTARKNMGR
                     NLAKESPVKADMVVGVPNSSLSAASGYAEASGIPYEIGLVKNQYIARTFIQPTPELRE
                     QGVRMKLSAVRGVVEGKKVIMVDDSIVRGTTSRRIVHLLKEAGAKEVHVRIASPPLKY
                     PCFYGIDIQTRKELIAANHTIDEIKECIGADSLGFLSEEGLIESIGLEKDAPYSGLCM
                     AYFNGDYPTPLYDYEEKYQESLKEKVSFF"
     gene            35415..36467
                     /locus_tag="SCJ86_000364"
     CDS             35415..36467
                     /locus_tag="SCJ86_000364"
                     /EC_number="6.3.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306859.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylformylglycinamidine cyclo-ligase"
                     /protein_id="HEG1167952.1"
                     /translation="MNSMANAYAKAGVDVEAGYEVVERIKKHVKKTERLGMMGAIGGF
                     GGCFDLSVFQLKEPVLVSGTDGVGTKLAVAIEADKHDTIGIDCVAMCVNDILAQGAEP
                     LYFLDYLATGKNDPLRLENVVKGVADGCVQGNMALIGGETAEMPGMYSGNDYDLAGFA
                     VGIAEKSQLLTGETINPGDVLLGIASSGIHSNGYSLVRKIFFEQQQFSLSSKLPELKK
                     TLGEELLEPTKIYVSSLLPLIKEKKIKGAAHITGGGFVENIPRMLPESMAAQIKLGSW
                     PILPIFGCLQKYGEIPEMEMYEIFNMGIGMVLAVEESKVKEIVHEIEQAGEKVYEIGK
                     VIAKEKHRVVFIGEER"
     gene            36464..37042
                     /gene="purN"
                     /locus_tag="SCJ86_000365"
     CDS             36464..37042
                     /gene="purN"
                     /locus_tag="SCJ86_000365"
                     /EC_number="2.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005873397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylglycinamide formyltransferase"
                     /protein_id="HEG1167953.1"
                     /translation="MRIAVFASGNGSNFQALADYLSKKGLESSIDWLFCDQPEAYVLK
                     RATALSVPADCFSPKEFDSKKEYEEAILHKLKEKKIDLIVLAGYMRIIGPVLLKNYDK
                     RIINIHPSLLPAFPGLHGIRDAFEAGVKETGVTIHYIDQGVDTGPIIRQEKVRIEQED
                     TFDSLEEKIHRVEHRIYPEVISEIIENGGYLK"
     gene            37039..38574
                     /gene="purH"
                     /locus_tag="SCJ86_000366"
     CDS             37039..38574
                     /gene="purH"
                     /locus_tag="SCJ86_000366"
                     /EC_number="2.1.2.3"
                     /EC_number="3.5.4.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629746.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     phosphoribosylaminoimidazolecarboxamide
                     formyltransferase/IMP cyclohydrolase"
                     /protein_id="HEG1167954.1"
                     /translation="MTRALISVSDKTGIVAFAQGLIEQGVEIISTGGTKHALDKAGIK
                     TLSIEEVTGFPEMMDGRVKTLHPKIHGGLLGRRDLPSHMEAMETENIQPIDFVCVNLY
                     PFKETIMKPDVEIAEAIENIDIGGPSMLRSAAKNHEFVTSVVDPSDYEEVLSELRQYG
                     HTSLSFRQKLAAKVFRHTAAYDALIAQYLTDWVEEKEPEKLTLTYERKQTLRYGENSH
                     QAAAFYQSALLEKYSIAAAKQLHGKELSYNNIRDADAALRIMKEYDEPTVVALKHMNP
                     CGIGTASTILEAWEAAYEADPVSIFGGIIVLNREVDLPTAEQMHKLFLEIIIAPSYTQ
                     EALELLQKKKNIRLLTVDFAQEENQNREETVSVLGGLLVQDQDLAIENEEEWKVVTKR
                     KPTPAEAKALAFAWRAVKHVKSNAIVLANERQTVGIGAGQMNRVGSVKIAIEQAQEKM
                     EGAVLASDAYFPMDDSVEYAAKHGIKAIIQPGGSIKDQDSIDMANKYDLAMVFTDVRH
                     FRH"
     gene            38590..39864
                     /gene="purD"
                     /locus_tag="SCJ86_000367"
     CDS             38590..39864
                     /gene="purD"
                     /locus_tag="SCJ86_000367"
                     /EC_number="6.3.4.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288007.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylamine--glycine ligase"
                     /protein_id="HEG1167955.1"
                     /translation="MNILVIGAGGREHAISKKLLASPKVDTVYCAPGNPGMTKDGINC
                     VAISERDHSALIHFVKKEAIDWSIVGPEVPLLNGIVDDFMAEGLKIFGPTKAAALIEG
                     SKSFAKQLMDTYQIPTAKSRSFECYEKAAAYIKENGAPIVVKADGLAAGKGVVVATTV
                     KEALDAAEQMLNQHRFGDSSKKIVVEEFLAGEEFSLLAFVRNTKVYPMVISQDHKRAF
                     DNDKGPNTGGMGAYSPVPQISKKMIEKAVEEVLKPAALGMKELGRSFTGVLYAGLIAT
                     KEGPKVIEFNARFGDPETQVVLSRLKSDFAQVIDDLLENRTVELKWQQEGYNVGVVVA
                     AAGYPEEYETGMVLPDFLEEELSVYYAGVSSNNGKLVGAGGRLYLVEAYGETIKEAQK
                     KIYTALMDKTTDGTFYRTDIGEKAANSELIVK"
     gene            39986..40900
                     /locus_tag="SCJ86_000368"
     CDS             39986..40900
                     /locus_tag="SCJ86_000368"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631494.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HEG1167956.1"
                     /translation="MKKIQLGTSDQKAAAVILGCMRINNAEDPVKVIETAYENGIDFF
                     DHADIYGNGECEEIFADALAKTSIKREDLFIQTKCGIVPGVMFDFSKKHIIESVEKSL
                     TRLKMDYVDALLLHRPDTLVEPEEVAEAFDELERSGKVRYFGVSNQKPMQIELLKKYV
                     RQPLVANQLQFGLKHTGMIDQGIHVNMTDEASIDHDGSVLDYSRLHDMTIQAWSPYQY
                     GFFEGVFIGNEKFPELNQTLEELAAKYQTTPTGLASAWILRHPANMQVIAGTMNVGRI
                     KEIAQASEINMSREDWYELYRAAGNILP"
     gene            complement(41033..42598)
                     /locus_tag="SCJ86_000369"
     CDS             complement(41033..42598)
                     /locus_tag="SCJ86_000369"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375386.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HEG1167957.1"
                     /translation="MYKVFIIEDEYLIRNNLREQITVLGSYYPITYVGEAGDGEMALS
                     SIIDLQPDIILTDIQMPFMDGLTFSKEARKLLPWTRLIFISGFDDFDYMKGAIQVQAD
                     DYLLKPIKSSELKQAIEKAVTALDLQQKETHTSSDLVFELKKNHFLNGLFKGSLTISE
                     TLKLAESFNRSLAGKKMTVLLAANQVSTDFEDYAHLSDYLKFLFRENQQVIFSAVSSR
                     FIKFLIVDSLEENVIETSYQVAQTLVHELEKGQKNNFSVAIGPVAERISELPASFEIT
                     QMILETASSLKEQIISYEDLIQEAKLPVDSVFYFDLSLDLSSLSKNEVSKYIEHLTKP
                     QGTPTRTRLYRLFVLTKLLQHVCLKEKKIPQEMVHCTSTSQRLAISADDSLYRSTVYM
                     FISFLTALPSQPSRNKYQPILNHALDFIKENFTDPDMSLTWVAQQVALSPSHFSTIFS
                     QSFNQTFIDYLTEQRIELAKKLLQQSDYRISDIAFEIGYNDPNYFSYLFKKKQGISPK
                     EYRTQLFLKNKNN"
     gene            complement(42591..44042)
                     /locus_tag="SCJ86_000370"
     CDS             complement(42591..44042)
                     /locus_tag="SCJ86_000370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008267161.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HEG1167958.1"
                     /translation="MTKNLSIKQIFAQANKAMLLFAVIPLLLSILLYSQQLLIYQQTI
                     ANIQKADAIALKVDQKVLESMWDLISGQVTVDEFNEENILEELRNDIREIKNNTNTLK
                     EISTLDVSLRTIDTLEDYQNELINNILAKDFMDTNEEIINQVDSVTQLLSDILQDFVR
                     VEIDLASKKNTTLVRSLILLTIVELGVLSTIFFYAKKNRRFLERKVQEPINQLIEMSE
                     QLSSGNLGFRLTLPETLELQTLTASMNKMAEDLNQSLRENALKQYHLAQSEVRVLQAQ
                     ITPHFIYNSLDAIISLIEQDCFEEALTMTHALSGFFRTSLSKGQDWIPLEKEISHIND
                     YLVILKIRYGHMLDYKIDIPETLFPVSILKMILQPLIENAVYHGTKYVRHKGFIRITA
                     CKIDEMIQFKVIDNGIGIPAEKLAEIRKELAKGIDSDFSIGYGLYNVNKRLLLYYGND
                     QKPITIDSKYREGTTVTLMIPLSPKTIGGNKHV"
     gene            complement(44056..45072)
                     /locus_tag="SCJ86_000371"
     CDS             complement(44056..45072)
                     /locus_tag="SCJ86_000371"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315152.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HEG1167959.1"
                     /translation="MKRVLFFLALVVIAYQMMGCTPNTKEAPDPLVVGFSQSGTESSW
                     RKKHTESIITALEKEDYQVLYRNGYMNQERQIQDIRTFIAYKVDMIIFTPLQESGWDS
                     VLKEAKRAGIPVILVDRHIQTNDPELFVTHIGPSFKAEGNRAGLFVSNHFTNSKKQEI
                     RILELAGSENSTPTKLRSEGFLESINRKPTLKKDGTIDHKPPMTIVHRIVGDFIRMKG
                     KDQMKRYLETNDLSEIDVLYSHSDEMTHGALAAIKETSIKPGEDLIIVTIDGQEDMIE
                     KLRSGIVNCVVECKPDVGWYVANTVTRYFGNIQTGKTLPKDIFISETVFSQQNIANIP
                     TRNY"
     gene            45292..46380
                     /locus_tag="SCJ86_000372"
     CDS             45292..46380
                     /locus_tag="SCJ86_000372"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738724.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar-binding protein"
                     /protein_id="HEG1167960.1"
                     /translation="MKNKFRKIVGTIAILTASILLSACGNSGSADDSTGYVGIAMPTK
                     SAERWIADGNNMVSELEKLGYKTDLQYGEDKVENQVAQIENMITKGVDTLVIASIDGS
                     ALTDVLAKAKEADIKVIAYDRLLMNSENVDYYATFDNFGVGVSQAAYIEEHLGLKEGK
                     GPFTIELFGGSPDDNNALINYNGVMSVLQPYMDNGQLVVPSGQTSFSQIATLRWDGST
                     AQARMDNLLSANYTDQTLDAVLSPYDPISLGIISSLKGVGYGSESKPLPVITGQDATV
                     AGVKSIIAGEQTQTIFKDTRILAKNTIEMIKAISDGEEVPVNDTETYDNGVKTVPTYL
                     ANTVSVDKDNYQAELIDTDYYKESDLKN"
     gene            46434..47960
                     /locus_tag="SCJ86_000373"
     CDS             46434..47960
                     /locus_tag="SCJ86_000373"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315151.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter ATP-binding protein"
                     /protein_id="HEG1167961.1"
                     /translation="MADCILKMADITKKFSGIRALDCVNLMIERGEIHALCGENGAGK
                     STLMNVLSGLYPYGSYDGEIFYDGKLCQFKDLKDSEEKGIVIIHQELALSPYLSIKEN
                     IFLGNEQAERGVIDWELTEKKTYELLNKVGLSIDPNLLVSQIGVGQQQLVEIAKALSK
                     SVRLLILDEPTAALNEEESANLLRLIRELKEQGVTSIIISHKLNEIVDIADRITILRD
                     GQTIETLENEMISEEHIIRGMVGRDLVDRYPERKPDIGEVYFKVEDWTVYHPLDHERI
                     INDHINLHLKKGEIVGIAGLMGAGRTEFAMSLFGHSYGSKISGRILKEGAEISVKTVP
                     SAIANGLAYVSEDRKSLGLNLLMDIRENVTLSSLDKISQKGVLDKEKEVTEAENFRKK
                     MRIKTNSIHQIVSSLSGGNQQKVVLSKWLMTQPDILFLDEPTRGIDVGAKYEIYTIIE
                     EMAANGKAVCLISSELPEILGMCDRIYTMNEGRITGEVSREDADQEVLMKLMTKEEVG
                     "
     gene            47962..49167
                     /locus_tag="SCJ86_000374"
     CDS             47962..49167
                     /locus_tag="SCJ86_000374"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374439.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HEG1167962.1"
                     /translation="MEQAKNLTDKKEKKLNLKSRAADLFGRYSMVIILAGLLIAFQLM
                     TDGIFWRPLNITNLVLQNSHILVLAAGMLLVVLLGHVDLSVGSVMAFVGAIAGMMMVN
                     RQMNPWMTVIACLAVGGLIGAWQGFWVAYAGIPAFIVTLAGLLMFRGLTQVVLGGQSL
                     APFPAVFQKMSTGYLPDLSEGSSLHFLTLILGMIITIVLVVGQWRTRNRRKNHLFEVE
                     SPTAFLVKAVFTAVVVLGITYIFASYQGYPIILIILGLIVAAYGFLTNKTVAGRQIYA
                     TGGNRKAAELSGIKTKRITFWVFVNMGVMAALAGLVLAARLNAATPQAGTSMELDAMA
                     AVYFGGASTSGGIGTIMGAIVGGLVMGVLNNGMSILGVGVDWQQAIKGLILLLAVVLD
                     LYNKKKRTT"
     gene            49424..50401
                     /gene="guaC"
                     /locus_tag="SCJ86_000375"
     CDS             49424..50401
                     /gene="guaC"
                     /locus_tag="SCJ86_000375"
                     /EC_number="1.7.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003127210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GMP reductase"
                     /protein_id="HEG1167963.1"
                     /translation="MKVFDYEDIQLIPNKCIVNSRSECDTTVTLGKHTFKMPVVPANM
                     QTIIDETIAEFLAENGYFYIMHRFDEAARIPFIKKMKKRGLITSISVGVKKEEYSFIE
                     KLAEESLNPDYITIDIAHGHANSVIDMIQHIKKYLPETFVIAGNVGTPEAVRELENAG
                     ADATKVGIGPGKVCITKIKTGFGTGGWQLAALRWCAKAARKPIIADGGIRTHGDIAKS
                     VRFGATMVMIGSLFAGHEESPGETKVENGIVYKEYFGSASEFQKGEKRNVEGKKIWIQ
                     HKGSLKDTLVEMQQDLQSSISYAGGRDLEAIRKVDYVIVKNSIFNGDTI"
     gene            50665..52089
                     /locus_tag="SCJ86_000376"
     CDS             50665..52089
                     /locus_tag="SCJ86_000376"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298994.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PLP-dependent aminotransferase family protein"
                     /protein_id="HEG1167964.1"
                     /translation="MWEKVEKKQGPIYRQIMEQIMREIENGRINPGSQLESERKLGQL
                     FGVNRSTVVHALEELRELGVLTSKRGSGWFVNQTEWGKFAVPRIDWRQMISPRYEQTD
                     WYEQKIREAKQIQKTSFLDLYASEMPDELLPNFEFPSYSLEEILAEEKEMDLLGYTPL
                     RNKIKRYLESKFELAFSPNQLLVTGGGQQAIFLILQTILSAGEGIAVESPSFFYRLPL
                     FKASGTRLFGIPMDEEGIDLEKLKESIHKNKLKAVLINPNFQNPTGKVMSQKRREKLV
                     ELCRQYRLPIIEDDVFTDLSFLNQEEVLPIRTIDPDNVLYVGSLSRVLGKTTKIGWVI
                     GPETFISQLAQAQEMMEFSMNILTQITVANVFNEAIDLKMQDVRKKLKEKSRLISAWG
                     DSQSFFKLHSILGGYYAWITWDGKKLDPELAEKVVDMGLGIAPSLLFGQDINGIRINY
                     SRLDENQIPLFYSKMKILEKWLTE"
     gene            52550..53659
                     /gene="pdhA"
                     /locus_tag="SCJ86_000377"
     CDS             52550..53659
                     /gene="pdhA"
                     /locus_tag="SCJ86_000377"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016625315.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate dehydrogenase (acetyl-transferring) E1
                     component subunit alpha"
                     /protein_id="HEG1167965.1"
                     /translation="MANKKAPIDFQALLDEVNADFPVYQALDQDGNVVNKDLVPDLSD
                     DELVELMTHMVWSRVLDQRSTALNRQGRLGFFAPTAGQEASQLASAYAFDKEDVLLPG
                     YRDVPQLVKHGLPLSQAFLWSRGHAAGNSYPEELKALPPQIIIGAQYVQAAGVALGLK
                     KRNKKNVVFTYTGDGGSSQGDFYEAINFAGAYHANAVFYIQNNGYAISTPREKQTAAK
                     TLAQKAVAAGIPGIQVDGMDPLAVYTVSKMAREWAISGNGPVLIETLTYRYGPHTLSG
                     DDPTRYRTKDTDDDWQKKDPLIRFRKYLTEKGLWSEEKEEQVIEATKEEIKAAIAEAD
                     KVPKQKVSDFLKNMFEVSPQSIKEQIAIYEAKESK"
     gene            53662..54639
                     /locus_tag="SCJ86_000378"
     CDS             53662..54639
                     /locus_tag="SCJ86_000378"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005876487.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-ketoacid dehydrogenase subunit beta"
                     /protein_id="HEG1167966.1"
                     /translation="MAQKTMIQAITDALALELENDENVVVFGEDVGKNGGVFRATEGL
                     QEKFGEDRVFDTPLAESGIAGLSFGLALEGFRPVPEIQFFGFIFEAMDEVVAQMARTR
                     YRMSGTRNLPITIRSPFGGGVHTPELHSDNLEGLIAQSPGIRVVIPSNPYDAKGLLIS
                     SIRSNDPVVFLEHMKLYRSFREEVPDEAYEVPLDKAAVTREGTDVSIITYGAMVREAI
                     KAADNLAKENISVEIIDLRTVAPLDVETIIQSVEKTGRVVVVQEAQRQAGVAAQVVSE
                     ISERAILSLEAPIGRVSAPDTVFPFGQAENVWLPNAKDIEDKVKEIAEF"
     gene            54664..56307
                     /locus_tag="SCJ86_000379"
     CDS             54664..56307
                     /locus_tag="SCJ86_000379"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374447.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrolipoyllysine-residue acetyltransferase"
                     /protein_id="HEG1167967.1"
                     /translation="MAYQFKLPDIGEGIAEGEIVKWFVKPGDTINEDDTLLEVQNDKS
                     VEEIPSPVTGTVKNVIVPEGTVANVGDVLVEIDAPGHEDNEGDSGVAAESQTPAKPAA
                     EPTVDTESAGSSSEGVFQFKLPDIGEGIAEGEIVKWFVKPGDTINEDDTLLEVQNDKS
                     VEEIPSPVTGTVKNVIVPEGTVANVGDVLVEIDAPGHNSAPSTSAPSAEAPKEKVETS
                     GSASVVEAADPNKRVLAMPSVRQFAREKDVDISQVTATGKGGRVTKEDIENFLAGGPS
                     SAPAKSEAPEAAAPKEAAPAAESKPAAPAKPFKSNLGDLEERVAMTPTRKAIAKAMVN
                     SKHTAPHVTLHDEVEVSKLWDNRKRFKEVAAANGTKLTFLPYVVKALTATVKKYPVLN
                     ASIDDANQEIVYKHYYNIGIATDTDHGLYVPNVKDADRKGMFAIADEINEKAKLAHDG
                     KLSAEDMRNGTITISNIGSVGGGWFTPVINYPEVAILGVGTIAQQPIVNAEGEIVVGR
                     VMKLSLSFDHRIVDGATAQQAMNNIKRLLADPELLMMEG"
     gene            56315..57721
                     /gene="lpdA"
                     /locus_tag="SCJ86_000380"
     CDS             56315..57721
                     /gene="lpdA"
                     /locus_tag="SCJ86_000380"
                     /EC_number="1.8.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735668.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrolipoyl dehydrogenase"
                     /protein_id="HEG1167968.1"
                     /translation="MVVGDFAVELDTVVIGAGPGGYVAAIRAAEMGQKVAIIEREYIG
                     GVCLNVGCIPSKALIAAGHHYQESLDSEMFGVTSENVKLDFAKTQEWKENKVVHTLTS
                     GVGFLLKKHKVETIEGEAFFVDDHTLRVIHPDSAQTYSFNNAIIATGSRPIEIPGFKF
                     GGRVLDSTGGLALKEVPKKFVIIGGGVIGAELGGAYANLGAEVTILEGSPQILPTYEK
                     DLVKLVEDDFKKKGVTVVTNAMAKEAVDNGDSVTVKYAVDGKEESVTADYVMVTVGRR
                     PNTDDMGLEQAGVEVGERGLITVDKQGRTNVPNIYAIGDIVPGAALAHKASYEAKIAA
                     EAISGKKVAVDYKAMPAVAFTDPELASVGMTIKEAKDAGIEAKAYKFPFSGNGRALSL
                     GKTEGFIRLVTTIEDNVLIGAQIGGVGASDMVSELALAIESGMNAEDIALTIHPHPSL
                     GEIVMDASELALGLPIHI"
     gene            57883..58158
                     /locus_tag="SCJ86_000381"
     CDS             57883..58158
                     /locus_tag="SCJ86_000381"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292206.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UPF0223 family protein"
                     /protein_id="HEG1167969.1"
                     /translation="MQEYQYPLDLDWTTDEMVIVMKMWEDLERANEKGINNEAFLKTY
                     QQFKTVVKSIGEEKRLGREFEKASGYSLYRTVQQAKKNPNKTLRMNG"
     gene            58164..58931
                     /locus_tag="SCJ86_000382"
     CDS             58164..58931
                     /locus_tag="SCJ86_000382"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628723.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inositol monophosphatase family protein"
                     /protein_id="HEG1167970.1"
                     /translation="MEQMIDHIKSWLEEAGEKIKSSQQTLTISQKSNRTDLVTNVDRN
                     IQNFLIEKIHSFDPEAKILAEEEGCNQLSDLDGRVFIIDPIDGTLNFVIEGENFCIML
                     AVYESGVGKLGFIYDVMRNELFWGGKGIGVYKNDEPLPRPANKSLSEGLLGVNSYLFG
                     HNKYGIREIAESSMGTRMYGCAGLELVAILKGNHIAYISNLSPWDYAAGNVLLEEFGM
                     VTSGLFGTPLTFEKREYYLAGTSCAYAEIADLLKKEG"
     gene            59099..60928
                     /gene="typA"
                     /locus_tag="SCJ86_000383"
     CDS             59099..60928
                     /gene="typA"
                     /locus_tag="SCJ86_000383"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016176758.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="translational GTPase TypA"
                     /protein_id="HEG1167971.1"
                     /translation="MNYRNDIRNVAIIAHVDHGKTTLVDELLKQSDTLDAHTQLQERA
                     MDSNALEKERGITILAKNTAVDYKGIRVNIMDTPGHADFGGEVERIMKMVDGVVLVVD
                     AYEGTMPQTRFVLKKALEQHITPIVVVNKIDKPSARPEHVVDEVLELFIELGADDDQL
                     DFPVIYASALNGTSSLSDDPADQEPTMAPIFDTIIEKIPAPVDNSDEPLQFQVSLLDY
                     NDYVGRIGIGRVFRGTIKVGDQVALIKLDGTVKKFRVTKLFGFFGLKRLEIQEAKAGD
                     LIAVSGMEDIFVGETVTPVDHQDALPILHIDEPTLQMTFLVNNSPFAGREGKFVTARK
                     IEERLMAELQTDVSLRVEPTNSPDAWTVSGRGELHLSILIENMRREGYELQVSRPEVI
                     EKEIDGVKCEPFERVQIDTPEEYMGSVIESLSLRKGEMQDMVHTGNGQIRLTFLTPAR
                     GLIGYSTEFLSMTRGYGIMNHTFDQYLPMLPGQIGGRHQGALVSIDTGKATTYSIMSI
                     EERGTVFVEPGTEVYEGMIIGENSRDNDLTVNITKAKQMTNVRSATKDQTSVIKKPKQ
                     LTLEESLEFLNDDEYCEVTPESIRLRKQILEKNAREKASKKKK"
     gene            61189..62961
                     /gene="recQ"
                     /locus_tag="SCJ86_000384"
     CDS             61189..62961
                     /gene="recQ"
                     /locus_tag="SCJ86_000384"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA helicase RecQ"
                     /protein_id="HEG1167972.1"
                     /translation="MNDLLKQYFGYDEFRPGQKEIIQKVIDQENVLGIMPTGSGKSIC
                     YQLPALLLDGLTVVVSPLISLMKDQVDAANQLGIPATFINSSLDGYETARRFQEIDRQ
                     QYRLLYIAPERFIMPDFIQAMKRWNVRMIAIDEAHCISQWGHDFRPSYLQMANQLDQL
                     PNRPVIVALTATATVQVAADIKRLLKIPENNHIQTGFERENLRFQVIKDQKKEQYLIE
                     YLKINKNQSGIIYAATRKEVDRLYHLLKKFDFSVGRYHGGLNENERTEMQEAFLYDRL
                     QLIVATNAFGMGINKSNVRFVIHYQIPGSLEAYYQEAGRAGRDGLSSEAILLFSPQDI
                     QVQKFFIQQSQREEGQKQKEYEKLKAMTEYVYIESCLQQYILNYFGETSSPCNRCGNC
                     LDDREIVEVTTEAQMVLSCLKRMGENYGKQMLMKVLAGSKEQKLQALGFGHLSTYGLM
                     KNQSQKETMQLIEYLISNGYLLTINGEYPVLKVTERGIQVLKGQESVYRKEPKKVQQL
                     SDEETDTLFEVLRELRTDLASEAGVPPYVVFSDSTLKEMSRIRPSSRLEMLQIKGVGQ
                     SKLDKYGEAFLSRIKNADSNVLDK"
     gene            63075..63377
                     /locus_tag="SCJ86_000385"
     CDS             63075..63377
                     /locus_tag="SCJ86_000385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298990.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YlaN family protein"
                     /protein_id="HEG1167973.1"
                     /translation="MDSLSKKLAIQVLNDEAERIKRLIRNQNNSLCISQCKAFEEVVD
                     TQMYGFSRQVGFAVRIGVIGKNEGQLMLSELERELNQLYSEVYQEIYDKKEVGKEE"
     gene            63380..64543
                     /locus_tag="SCJ86_000386"
     CDS             63380..64543
                     /locus_tag="SCJ86_000386"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330211.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FtsW/RodA/SpoVE family cell cycle protein"
                     /protein_id="HEG1167974.1"
                     /translation="MPKKVKKRHLLDYSILIPYLILCVLGLIMVYSSTSYLLLENGQN
                     PSASVINQSIFWVLSLIAIALLYKMKTDVLKNQRLIMAAIAVLTILLLIVVFFGKEIN
                     GAKGWLQIAGFSIQPAEYLKIISIWYLSLTLSKRQNSVQKDFLGTVKRPLAMVIGLTA
                     LVAILPDFGNAAVIFLIILVLLLASGVNYVYTLIVGVGGFCLSTFTIWLINITNGKIL
                     PGRLQYIYNRFAIYQNPFSDELNKGHQLVNGYYAMFNGGLFGRGLGNSIQKKGFLQEA
                     QTDFIYAIVVEELGVIMGILILALLFFMIVRIVLVGIRSKDPFNSLLCIGIGAMFLIQ
                     VFVNLGGITGVIPLTGITFPFLSQGGSSLLMLSICVGFVLNISADEKRKSLGL"
     gene            64569..67997
                     /locus_tag="SCJ86_000387"
     CDS             64569..67997
                     /locus_tag="SCJ86_000387"
                     /EC_number="6.4.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327081.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate carboxylase"
                     /protein_id="HEG1167975.1"
                     /translation="MKKVLVANRGEIAVRVFRACTELGIKTVGIYAEEDEYSVHRFKA
                     DEAYLVGQGKKPIDAYLDIEGIISIAKECGADAIHPGYGLLSENLNFAQRCEEEGIIF
                     VGPKLHHLDIFGDKIKAKAAAIEAGIASIPGTDGPIASIDDALEFAKQYGYPIMIKAA
                     LGGGGRGMRVAHDEKSAREGYERAKSEAKAAFGSDEVYVEKYIANPKHIEVQILGDTH
                     GNVIHLFERDCSVQRRHQKVVEVAPCVSMNEQQRQKICQAAVQLMKYVGYVNAGTVEF
                     LVEGDDFYFIEVNPRVQVEHTITEMITDIDIVTTQLLIAQGLDLHKEIGLPQQEGIKL
                     NGSAIQCRITTEDPLNNFLPDTGKIDTYRSPGGFGVRLDVGNAYAGYVVTPYFDSLLV
                     KVCTHGATFETAIQKMERCLKEFRIRGVKTNIPFMLNVITHPEFQSGNAKTTFIDSTA
                     TLFEFPRLRDRGNKTMKYIGEITVNGFPGIESGEKPFYEEPRMPKDLITRTDYVTAKN
                     VLDAKGADALVEWIKGQENLLLTDTTFRDAHQSLLATRVRTKDFKQIARLTGEGLPEL
                     FSSEMWGGATFDVAYRFLNEDPWQRLRKIRSLMPNTLLQMLFRGSNAVGYSNYPDNVL
                     VEFVKEAAAQGIDVFRIFDSLNWTPQMEKSIQAVRDTGKIAEAAICYTGDINDPSRAK
                     YNVQYYKDMAKELEQLGAHIIAIKDMAGLLKPQAAYRLISELKETTDLPIHLHTHDTS
                     GNGIITYSAASKAGVDIVDVAMSAMSGNTSQPSMSSLYYALVNGPRLPEITIENAQKL
                     NHYWEDVRMYYKPFENGLNAPETEVYMHEMPGGQYSNLQQQAKAVGLGHRWDEIKQMY
                     HTVNLMFGDIVKVTPSSKVVGDMALFMVQNDLTEEDIYEKGETLSFPESVVTFFQGEL
                     GQPVGGFPKKLQKIILKGRPALNERPGLLAESVDFNEVKKELAEKIGYEPKQDEVLSY
                     LMYPQVFLDYQKAYNQFGDVTLLDTPTFFQGIRLGETINVQIERGKTLIIRLDEIGEP
                     DIEGNRVLFFNLNGQRREITIKDNSIISTVQTRLKAEPTNREQIGATMSGSVLDVLVK
                     KGDNVKKGDTLMITEAMKMETAIEARFDGEVAHVYVSSGDTISSGDLLIEVTEK"
     gene            68018..69142
                     /locus_tag="SCJ86_000388"
     CDS             68018..69142
                     /locus_tag="SCJ86_000388"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292199.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEG1167976.1"
                     /translation="MKRLGLFWLILLLVIVAFYMEPVLFPPKLEVKENSDQQISNQMT
                     TWKYQELKATGFSTYIGKPIDQLEKDFGQAYDRLTSGFGFETRYYENKASHLTFEANI
                     QDSRVTTVKVLQTGPADIAPFYLGMTMQDLTKITTIYTNFTFEYHDTEVGIELMEEDM
                     NYRPLIAFDNDTFAILFFDQTTNGLFSVVYLDKDSLLKLLPYQVFGEGLPHYQADKSA
                     DWESINRVKERKSTTLLNMLRREDELPIYNQTLAFQNHSQLLLHDFLTRPEEILSEER
                     MADWQKSLGSAQTTVKFSLTNDELEKLISKQKLENTSGLFIHPAIDATFSFLYWYSDP
                     YNHDRFMDPGTDSVGIAFSKENMLVLLQEENGESSDSSDR"
     gene            69132..69569
                     /locus_tag="SCJ86_000389"
     CDS             69132..69569
                     /locus_tag="SCJ86_000389"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305283.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YlbF family regulator"
                     /protein_id="HEG1167977.1"
                     /translation="MIVNEELFQLEDQCLQVAEMIKKSKTMASYLEHKQSMEESAKVC
                     QLKEAFLSKKEAFENVAAYGEYAPDYREKKRAVRKAKRQLDMDEHVAAFRYSETQLQS
                     LLDQIVQVLAEEVSNEIKVDAGSPFFETKKHFGCGGNCHAIRQ"
     gene            69553..69891
                     /locus_tag="SCJ86_000390"
     CDS             69553..69891
                     /locus_tag="SCJ86_000390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297107.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YlbG family protein"
                     /protein_id="HEG1167978.1"
                     /translation="MQLDNSEFEITKRRGLIVWVYSLKQLKNLKKFGLLHYVSRRMKY
                     VVLYLNEETLEETEERIRKLHFVRNVERSYRPDIEMNFAEKIGNKTVLKDEGFEIEEL
                     NTEIRLAEHL"
     gene            70010..70570
                     /gene="rsmD"
                     /locus_tag="SCJ86_000391"
     CDS             70010..70570
                     /gene="rsmD"
                     /locus_tag="SCJ86_000391"
                     /EC_number="2.1.1.171"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340075.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (guanine(966)-N(2))-methyltransferase
                     RsmD"
                     /protein_id="HEG1167979.1"
                     /translation="MRIISGNYGGRRLKSLAGANTRPTTDKVKESIFNMIGPYFDGET
                     VLDLFAGSGGLAIEAVSRGCSHAVCVDKNYQALKIIKENIEITKEPEKFTTIKLDADK
                     AIERLAAEKQQFDYLFLDPPYAKQKIIDQIHKMEELGLFSKTAVIVCETDKTVELPET
                     VAGFHQIRKQIYGITAVTIYRKEGNQ"
     gene            70567..71058
                     /gene="coaD"
                     /locus_tag="SCJ86_000392"
     CDS             70567..71058
                     /gene="coaD"
                     /locus_tag="SCJ86_000392"
                     /EC_number="2.7.7.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374472.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pantetheine-phosphate adenylyltransferase"
                     /protein_id="HEG1167980.1"
                     /translation="MKRALFPGSFDPFTKGHLDTVERAAKLFDEVVIGVFINTSKKSL
                     FPPEERMTLITKAVSHLPNVKVMHQENQLTVETAKEIGADALIRGIRSIKDFEYEREI
                     AQMNHHLYDELETVFLLAKPEYSHVSSSILKEVLHFGGDVSSYLPPVINEALAGKRES
                     NES"
     gene            71048..72091
                     /locus_tag="SCJ86_000393"
     CDS             71048..72091
                     /locus_tag="SCJ86_000393"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374474.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PDZ domain-containing protein"
                     /protein_id="HEG1167981.1"
                     /translation="MKAKQWLKTVLIIIFGMLIIGFVVVPLPYYVESPGATIDLKELI
                     TVNDKEDKGSGSFSLTSVGIRQATGFLALKAKMTPFEELISEEELTGGATNEEYNQMQ
                     QYYMDSSQNAAIEQALKLADKPYKMEFKGVYVMSVEKTSNFFGKLSVGDTVTKVDGKS
                     FQSTEAFMDYVKSQKVGQTIEISYLHNGEEKTASGDLIELPTDKKAGIGITLTDHTEI
                     ESDTDVRINSGSIGGPSAGLMFTLEIYEQVTGKNLRHGKQIAGTGTINSEGEVGRIGG
                     IDKKVASADKAGVEIFFAPDDTISDDVKKEYPEIKTNYEEAKAAAKKLNSSMKIVPVK
                     TVQEALDYLEKMK"
     gene            72250..72978
                     /locus_tag="SCJ86_000394"
     CDS             72250..72978
                     /locus_tag="SCJ86_000394"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016925838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-hairpin-helix domain-containing protein"
                     /protein_id="HEG1167982.1"
                     /translation="MKGVMNDFYWSFFEKRTMIEKISKKILASPKLIIWMSMIVLIFC
                     IASGFLLSNLLFYRETEETAAKEILSSFSSSGEDPSAEGNDVEEDARPKVMYTDIKGS
                     VKEPGIYSFSSEERVYDVLKRAGGLLEEADSDRINFSAKIEDQQVLYIPAVGEELPEH
                     LDQLASSEGKQPTADSEPPKININTATPSELQQIPGIGSVKAQEIIRFREENGSFQKV
                     EDLQEISGIGEKTVEKLKNFVTIK"
     gene            73094..73339
                     /locus_tag="SCJ86_000395"
     CDS             73094..73339
                     /locus_tag="SCJ86_000395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336971.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SGNH/GDSL hydrolase family protein"
                     /protein_id="HEG1167983.1"
                     /translation="MIVRKETLKKPMLNVYLQNKISGIHIMNTAVSGNNSQALRERFA
                     KDVLSYTADKVFILIGTNDLAEHKQLSKETYQKICSG"
     gene            73336..73554
                     /locus_tag="SCJ86_000396"
     CDS             73336..73554
                     /locus_tag="SCJ86_000396"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318439.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="esterase"
                     /protein_id="HEG1167984.1"
                     /translation="MIVQLKKDYSSDHAFFITPPAVDETKQKYRNNKLIKEYISVLTE
                     VAKRHDCEVSIFIRNFLNKWIRRIFIMG"
     gene            73690..74229
                     /locus_tag="SCJ86_000397"
     CDS             73690..74229
                     /locus_tag="SCJ86_000397"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292191.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ComE operon protein 2"
                     /protein_id="HEG1167985.1"
                     /translation="MLEKSSKACDTNKEKVTDKSHNERIPWDQYFMAQAVLLSLRSTC
                     TRLEVGATLVREKRIIAGGYNGAVSGDVHCIDEGCYVVDGHCLRTIHAEMNALLQCAK
                     LGIPTEGSEIYVTHFPCLACTKALLQAGVKGINYLHDYRNDPYAQALIEQLGVSVHQV
                     ALDSQHFSKLQNELTGKHV"
     gene            74222..76495
                     /locus_tag="SCJ86_000398"
     CDS             74222..76495
                     /locus_tag="SCJ86_000398"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375359.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA internalization-related competence protein
                     ComEC/Rec2"
                     /protein_id="HEG1167986.1"
                     /translation="MSSVLERHVRNRLIFPAITSMIICLLYYTDNWGYRLLLAGIFLW
                     FSLKEGRFIAYLCILCSITVSISYRLQEYSFLEEKTTSAQITVFTDTIRANGNFLTME
                     GRLSENKQKLQLSYQVNTEAELKTWLNFRGKGIELTAEGEFISPAKKRNLYNFDQEAY
                     FRNHRISGRFQIQKIEQITMQNHWRNWLQRKRGSFISFVQSEFPQKTSVYINSLLWGY
                     KDAGFREPEEIFRESGLLHLFSLSGLHIQFYLGWLYYLFRRIGWPLHVSIIPLSLLTI
                     CYVSIAGSSISVLRASLLFLLRLLVKLLNKHYSTMDLFAVVFWLLLLFDPRFLFHAGG
                     QLSFFMSFLFILISFDGDWLKKTASHVLFTMITMPLIVWHFYEWPILGSLFTLLIAPV
                     FKFLFLPGVLFLTLFNRWLPETLLLLIEEGLILFERIIDFSSGSSLIIGRLPLVLSGM
                     YIFGLLICMDRLKEQPVKHLFYVGLFSFIFLTLPFLRFSSTIAFIDVGQGDSIFLQSP
                     FRKETILIDTGGQLHFEREKWAQGMIRPPAERNIVPYLKGQGISVIDKLILTHDDTDH
                     VGELPTLSQHFTIKKIYIGWGAGRNEPLQRHLSEAQKNGTEIIEIKHGDRISGYYDLY
                     VLTPFEKGEGRNEDSIGLWMEYNNRRFLFLGDLNQAMEKQLLLIYPNLKADVVKLGHH
                     GSRTSSNTDFLSHIEAKHGIISCGVNNRYGHPHSEVLETLEENQIKTWRTDQQGMITY
                     RWHPVFHPHGLVETMID"
     gene            76533..77579
                     /gene="holA"
                     /locus_tag="SCJ86_000399"
     CDS             76533..77579
                     /gene="holA"
                     /locus_tag="SCJ86_000399"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335027.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit delta"
                     /protein_id="HEG1167987.1"
                     /translation="MVSNLSIQAELQKIRKEKLASCYLILGTEKFLQDQVRTEIVKKI
                     QLEGEDDLNFLSFDMENASLDEVVAEAETLPFFGDQRLVFVENPYFLTGEKVNNGIEQ
                     NTDLLTAYLKEPLESTVLVFFAPYEKLDERKKVTKQLKKTAIIINVQQLNEKEVRQYL
                     MNTLENSSIKMERSAIDLFLRLTDLDLSKMMRELQKLVLYGQNQQQITVREVEELVPK
                     TLEHNIFDMTQYILKGKTEQALRLYEDLVMQGEETIKINAILLSQLRLFLQTKFLVKI
                     GYQQANIAETLKIHPYRVKLAMQEVKKFDEHLLVQLFDQLVEMDYQIKTGQIEKELSF
                     QLFVLRTGQLLRNR"
     gene            complement(77668..77922)
                     /locus_tag="SCJ86_000400"
     CDS             complement(77668..77922)
                     /locus_tag="SCJ86_000400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016625292.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S20"
                     /protein_id="HEG1167988.1"
                     /translation="MPNIESAIKRVRTSENANVKNSSQTSAMRTAIKKFEDAVASGAD
                     NVDALYKEAVKAIDMAESKGLIHKNKANRDKSRLSKKIAK"
     gene            complement(78322..78591)
                     /locus_tag="SCJ86_000401"
     CDS             complement(78322..78591)
                     /locus_tag="SCJ86_000401"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEG1167989.1"
                     /translation="METFEGLLIDTYVLQEQPMMLTGINLQLEDRVINGLLKDRYLAK
                     YILRLPLNKYVLEVSGKWNRRNQLVIKKMLIKNIDEYIKIIGTPE"
     gene            complement(79061..79516)
                     /locus_tag="SCJ86_000402"
     CDS             complement(79061..79516)
                     /locus_tag="SCJ86_000402"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF441 domain-containing protein"
                     /protein_id="HEG1167990.1"
                     /translation="MESWLFLGIILLVAILAKNQSLMIAAAFVLIMKLIPASRSFFPW
                     LQGKGINIGVTIITAAILIPIATGEIGLRDLLEAFKSPIGWVAVFCGGLVAVLSSKGV
                     GLIASDPQITVALVFGTILGVVFLRGIAAGPVIASGITYCILQVFALGK"
     gene            complement(79654..81582)
                     /locus_tag="SCJ86_000403"
     CDS             complement(79654..81582)
                     /locus_tag="SCJ86_000403"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heavy metal translocating P-type ATPase"
                     /protein_id="HEG1167991.1"
                     /translation="MTNQKKAFAATIGCLLFMIFGFIFDRFSLTITPLFYISAIIIGG
                     YKQTSEGLKELWNDRTLNVDLLMALAAIGACLIGNYFEGAMLTFIFCLSGALEEYTTN
                     KSQKEITALMNLQPQKAQLLKENGQMQEVDVSQLKVDNLVFVAKGAAVPIDGFLESTQ
                     ATIDESALSGESVPVEKYLGDEMYAGTLNQGNPITIRVTKTSSETVFAKIIQLVEEAQ
                     NTPTQTASFIERIENNYVKLILLAVPLMILLPHFFLGWSWDESFYRGMVLLVVASPCA
                     LVASATPASLAALSNSAKNGILIKGGIHLEKLSELKAIAFDKTGTLTKGKPVVTDSFF
                     FDEKDLAQQLLVAMEQKTTHPLAHAILQYFDCTIPEEIQYLPIEEITGFGLQTTYLGA
                     QWKVGKHAYDPETMIISKEIAEMIERLENQGKTVIYLSKDQQLIAVLGLLDIPKANTQ
                     QVISYFKSQNIHTSMITGDHSGTAKAIAEQVGIEHYYASCTPEEKTQLVKKENELYQV
                     NAMVGDGVNDAPALAAASLGVAMGQGTDAAMDIADIVLMKSDLNKFVYSHRLSLKMRR
                     IIKQNIIFSILVILLLIASNFLQFLNLPLGVVGHEGSTILVILNGLRLLRPLPDLSDS
                     SKKCKSCPLYKARLSSVK"
BASE COUNT        26519 a        14041 c        17675 g        23526 t
ORIGIN      
        1 acagtaatgg gggaaaccgg ttggagagca accaccttga actatcagtg gccagtagca
       61 tttagtttac taacttttta tccgtttttt caactcttaa gaggagaaga aataaataga
      121 aaaatatatt gggtaagtat tcctttattg atatttttaa ctaatcaaga acaagtcaac
      181 gcctgttttt ttgttctaac aagtatagtt agtttatatt tgattgtcaa tggacgatat
      241 aattataagt tatcagtatt ttcaataata agcttagcag aattaatttt ctctttgaca
      301 actccaggga atgcattacg tgctgcacat gaaattaata aatggtttcc tgaatataaa
      361 aatttcaact ttcttaataa gttagattta ggtatttcat catttggaaa gccgtttttt
      421 ttagacatga atatcttatt tttattgcta tttttcttaa ttttccttct aacatatagg
      481 aaatgtcaaa actattatgt gcgtatattg actgcattac cgtttttctt aaacctgatt
      541 atttattttg gtaatacaat gggtcaaagc tttacttacg tcaatggaaa taaaagggca
      601 atgatttgga gtagtagtaa cctgaataat ctctttacag agttaggaac gaaactgtca
      661 cttttttatc caggtacttg gatagctact ttagtagtct tggcattatt actatgttta
      721 attgttggaa tttatttaag ttttgacaat aagaaaactt ctattttttt agttattttg
      781 atgataatgg gattttgttc aaggttaatt atgggatttt ctcccacagt atgggcttcc
      841 ggaatgcgga cgtactatat actttatgtt gttatcgcca tcttagtact aatggcagtt
      901 aaagagctaa tgaaaagcat gagtgtacaa aaaaatgaat ttatgcaatt tggattaacc
      961 gtgcttggta tttgtacctt tataataacg gtgataaaca gataatatac aaaaaatccc
     1021 tcgctttagt attaggtgag ggattttact cgtttttcga ttaattgttt taacacttgc
     1081 aagtttctac gagtagcttg tttccgacct tcatatactc acctcaggct gtttacatga
     1141 ttgtacgttc tttttgtaaa ggatacagtg atatgtatat aatggcataa agaaacgttc
     1201 aaacaaaaaa actcgtgaag caaaattaac ttcacgagtt gtagtgcaga tgatgggact
     1261 cgaacccaca cgagctaacg ctcacatgac cctcaatcat gcttgtctgc caattccaac
     1321 acatctgcgt aataataaca cactaatgat agcgaggttt tacatagttg tcaagctcaa
     1381 aaatgggagg acttattaag atggagaaat atgacggaga attttcagga ctgggaatga
     1441 tcttaggtgt attgattgga cttgcattcg gacgtttttt gcttggtttt atgctaggaa
     1501 tcatttgtgg aattgcaatg gattgggcag cgaatctatg gaatgactat catgacaatt
     1561 aagttctcaa cgcttgtttg tgttttttta atacttacgt ttttcctaaa taagtggcaa
     1621 aattttctag gatggagtat gatattgaaa tagtaaataa caaaaaattt ggaaagttct
     1681 aactttttga aatgagggat tggctaagtg atgagagaat ttgaaaaatc caacaaatta
     1741 gaaggtgtca gttacgatgt tcgcggtccc gttctagaag aagcggagcg gatgcaagaa
     1801 gagggcatta gtattttaaa attgaacacg ggaaatcccg caccttttgg atttgaagca
     1861 ccaaacgaag tcattcgtga tttgatcatg aatgcccgtg actcagaagg ctactcagat
     1921 tccaagggga tcttttctgc tagaaaagca attgagcaat attgccaact gaaacatttt
     1981 ccgaatgtga cgatcaatga tatctacacc ggcaacggag tgagcgaatt gatcacgatg
     2041 tgtatgcagg ggttgctgga caatggagat gaagtactgg ttccaatgcc ggactatccg
     2101 ttgtggacgg cttctattgc attagctgga ggcacacctg ttcattatat ttgcgatgaa
     2161 gaggcagaat ggtatcctga tattgatgat atcaaatcaa aaataacgag tcggacaaaa
     2221 gctattgtta tcattaatcc taataatcct actggggcac tttatccaaa agaattatta
     2281 gaagaaatcg ttgaagtagc tcgacaaaat aacctgatta tttattccga cgaaatctat
     2341 gatcgcttag tgatggatgg tctagaacat attccgattg ctacattagc gcctgatttg
     2401 ttcgttgtta cgctgaacgg tctatccaaa tctcatcgtg tagcaggatt tcgagtaggc
     2461 tggatggtcc taagtggaga taagtcacac gtcaaaggct atatagaagg gctgaatatg
     2521 ttgtcctcca tgcgtttgtg ttccaatgta ttgtctcagc aaattatcca aaccgcgcta
     2581 ggcggttatc aaagtgtcga tgagctgctc ctgccaggcg gaaggatcta tgaacaaaga
     2641 gaatatatct ataatgcaat caatgatatt cctggtcttt cagccgtaaa accaaaagct
     2701 gctttttata tattcccaaa aattgataca aagcgattca atattttgga tgatgaaaaa
     2761 ttcgttttgg actttttaca tgaacatcat attctacttg tacatggtgg tggatttaat
     2821 tggaaccagc cagatcattt tcgaatcgtt tatcttccta aaatggatga tttgaaacaa
     2881 acagctaaga aaatgagaga attcctagca acctatcgcc aaaaatgatt ttaaaaacca
     2941 aatttttcag taggagggcc acagacaaac acactgaggc tctctttcta gtggagtttt
     3001 cttaaataac taaagtatat tatttagttg caagtctttt cttgaagttt gcgacttcct
     3061 aaagtaaaat acgaatagaa tgcgaatacg catattttgg ggaggatact tatggaaacg
     3121 ttaatcgagt cctttggaac gatcattaaa gagattcgaa aagaacaaaa aatgacgcaa
     3181 aaaatgcttt cgcatgatat ttgttcacaa agtgtattaa gtcggatcga aaataatgag
     3241 gaactgccga atgtattggt gatgatgcag atttgtcaac ggctaggggt aacgattgat
     3301 catgtgatga atctctctcg aaaaactact cggacaaacg ataaattatt tgaactgttg
     3361 gatagttgtt tccaaaaacg tgattatcgg aagttagaac aagtactaaa atcttctgat
     3421 atttctgaaa atctgtatca agcaactgac tttcaacgct attattatta tctcggtgtt
     3481 tgcgaattca cgttgcgtca aaatatctct cagtcccttt attatttgaa agaagcatta
     3541 agctactcta gccaaaaaga aagaatccat gtttctgaca cagaaataca actgattagt
     3601 tgtatcggaa aaatatatgg tgtggcagga aaaacggctg aagcacgcta ctatttgagt
     3661 cgaagcatcc aactgctgca tcaatcagct gatgaacgat caaaaagtga attttcgcaa
     3721 attttttata attatggaaa tttcttattc catcaaaatg aaatcgacga agcgttagaa
     3781 caggtgaatc aaggaatcta ttgggctcaa gagaagaata gctactatta cctcaatgat
     3841 ttatttgtgc ttaaaagctt aatatacaag cgtaaagaag aaccagaaag agcagcattc
     3901 tatgaaggct tagcacaagc tgtgaaacaa atttcaaaaa atttgtaaat ggtgtagacc
     3961 attcttgact tcttctctaa ataagagtac tttagaggta gaaaaacaaa gggagagaat
     4021 agaatgagaa cttttatcta tgctgacaaa ttctttttag cgtcaggtgt caaacatgca
     4081 ggctatcttg aaattaccga tggacttttt ggtgaataca ccaaagaact acctgaggga
     4141 gaagtcaaca tcattgacca aagcggcaaa tggattgctc ctggtttggt agatacacat
     4201 attcacggct atatgaatca tgatgtaatg gataatgatg cagaaggcat caaagtgatg
     4261 tctgaaggtc tgttatcttg tggagtgact tcatttttac caacaacatt gacgtcaagc
     4321 aaagaacgtt taaaagatgt agctgagaca atcggcaaaa tgtatcagga agcaccagga
     4381 gctaaaatca aaggaatcta ttttgaaggt cctttcttca cagaagaaca caaaggggca
     4441 caaaacccaa gttatttcgg ggatcctgat ttggatactt tccatgaatg gcaagaagct
     4501 tcaggtggtt taatcaaaaa aattgcttta gcaccggaac gtcaaggtgt agaagagttt
     4561 gtcaaacaag tgactgaaga aggagtagtt gtttctctag gacacagtaa tgctacacta
     4621 gaagaagcgc aaaaagctgt tgaagcaggc gcaagtgtat ttgtccatgc tttcaatggt
     4681 atgcgtggat tgaatcatcg cgaaccaggt atggtgggtg cgttattatc tttagaacat
     4741 gtcttttctg aattgatttg tgatggccat catgtacatc caaatgctgc gggaatccta
     4801 atggaaaaag caggacatga tcatgtcgca ttaattacag actgtatgat ggccggtggg
     4861 atgcctgatg gaaattacaa tttaggcgaa tttccagtag tagtaaaaga aggaacagca
     4921 cgtctagaat ctggcaactt agctggaagt atattgaaat tgaaagaagc aattaaaaat
     4981 gttgtagact ggggattagc aacaccagaa caagcagtca tgatggctag tctgattcca
     5041 gcaatcagct gtaaaatcga tgatacatgt ggaaaaatcg ctcaaggacg cgatgcagac
     5101 tttatcgtat tgaaccctga tatgacattg gaagcaactt atctagatgg cgtggaacgt
     5161 ttccacgcat aatataattt aagttaacct taaaactaaa cttcttaagt ttggttttaa
     5221 ggtttttttg tgttctagta aaaatcactt tacataagtt tcaaaagttt ctttaatttc
     5281 ctattgttca ataagtaaca atgtaaagat aaaattaaaa atgttaatac gtattagcaa
     5341 aataaaagct ttttttattg tgaataaatg aacataatta ggaggagaaa gatgaactta
     5401 tttgacattt ttcgatcgac tttgacagat atgtcgattg tcagtgcaat cacttcaaca
     5461 gtttttatta tattattagg gtttgtttgt agaaaaaagg ggattttttc tgaatctgtt
     5521 ggaaaaattc tatcgaaagt cgtcttaaca gtggctttgc cagcactggc atttcgagct
     5581 ttcatgcaag atatcaatac agaattactg atgcaaggaa tcaatgtctt gatttgggga
     5641 ttagttattt atattattct tattctagtt tcaaaacctt ttttcttaag ctataaaggg
     5701 gataagcaaa caacgttgcg tgtcctgacg atctttggtt ctacgacctt tttcggaacg
     5761 cctatcgtca gtgcgattta tggtcctgtc ggcgtgatgt attcctcaat tttcaatatt
     5821 ggctaccgaa tctttttgta ttcttatgga tacataaaaa tgagtggctt gaagatggaa
     5881 gctaaaaata tcaaagcgat gttcgtcaat ccaatcatta tcgctacttt tgccggcctt
     5941 ttcatttggc tgttccaagg gtatctgcca caagtgtcag ttacaggtgc agaaggagag
     6001 ataagccaag tagcgttttt gcgaattgac cagactgcac cgtggctatt tcagccgatg
     6061 gattatttag ctggtttaag ttcaccattg gcgtggctag caatcggcgc aacattaggt
     6121 gaagtaagct tcaaaaaagc tgcatctgaa aaaacttcat ggtattacag tctagtcaaa
     6181 gtggtatttg tacctgcatt gaatatcgtg attctagcag ttttgacggc aacgaatatt
     6241 ctaccagtca attttgaagc actggcaaca atcgttatca tgatggctac accagctgca
     6301 acagtggcag cagcctatgc tatcagtttt gatagggagg cacttttagc ctccaatgca
     6361 tccttactgt caactatttt tgctgtggta ctgacacctg tatggatcgt tgtattacaa
     6421 atgattcatg gcataggttt attttcataa gaaatggagg aagaaagtat gagaatcgca
     6481 tgttacggtg ttcgtccgaa cgagatagat ttttttgaaa aattgaatat ttaccattat
     6541 gatctcagtc tttatgaaga actgttgaca catgacaata tcgtgtcagc aaaggaccat
     6601 gatgctgtat tgttgagggg aaattgtata gcagatgaga ccaatcttgc aaaaatgcag
     6661 gaatatggga ttcgttatgt ttttacacgg actgtgggag tcaatcatat cgatctacaa
     6721 gcggcagctg attttggtat gacagtagcc cgtgtaccat cgtattcgcc aaatgcgata
     6781 gcagagttat ctttgacttt tgccatgatg cttttgagaa atactgcgta tacgacgatc
     6841 cgaacatctt tcaaagattt tcgagtggat gagcaaatgt ttagtcgtga aatcagaaat
     6901 tgcacagtag ggatcatagg aacaggcagg atagggttga cggaagctgt tttgtttaag
     6961 ggactaggag ccaaagtgat cggctacgat attcatcaga cagaagctgc taaaaaagta
     7021 ttgacgttcc actcgcttga agagttatta gccaaaagcg atattgtcag tattcatgta
     7081 cctcatatcc caggtgaaaa cgatcaaatg atcaatgaag gatttttagc tcaaatgaaa
     7141 aaaggtagca tcttgattaa tacagcccgt ggagagcttc aagataatga agctatttta
     7201 agagctttga aaagcaatca tttagctggt tttgcaaccg atgtttttgc aaatgaaaca
     7261 gaggtttttt tccgatcctt caagccatgg gagacgattc cagaccctgc tatccagcaa
     7321 ttagtagaaa tgtaccctag agttcttgta accccgcatg taggttcaaa tacagatgag
     7381 gcattatcaa atatgatttc gacaagtttc gagaactttc gtgaaataat agaaactgga
     7441 aaaacgaaaa atgaggtatc tcttcctaaa gcccgtcaat taagataaga aaacatggat
     7501 attcgtcaat aaaatgttta aaaatagtcc aagtaaacag ttgttacttc tgtatacttg
     7561 gactattttg tatgggataa ttcgatgact caacagtaat ctgtgagctt tttccgccca
     7621 ttctttgtca attcataaca ggttagcggc tggccgattt ccaaatattt gattctttcg
     7681 ttcaatatat cttgttctac taaaaaattc aaatatttct tgacggatat acgtgacagt
     7741 gagctttttt gagccagttc ttttgttgaa aaaatagtat cttcttttag aataaatgca
     7801 cttatttttt ttaaagtcag ttttgacaga cctttaggca aatcgatgaa tgatggtatt
     7861 tcgtgaatgg gagtggtgtg gttgaatact tgatcgatca atttctgatc ggctttttca
     7921 gtcgtttgga ttgtctgaga gaaagcaaga tacttttcga tcgccagctg aaaacgttgc
     7981 aaagtgaacg gtttgatcaa ataatctaag gcaccgtagc ggatcacatt ctttatggtt
     8041 tcgatatcat ctgcagcagt gatcaaaatc actgggatat catttccttg ctgtctaagc
     8101 caagataaaa aatggatacc actttcgcca ggcatgtata catcaagcag cagcagatct
     8161 gcgttttttt gtctgaaata tttttttcct tcttctacat ttctgaaact ttcgattacg
     8221 ttcatattag ggatgctttc cacaaaagta cgattcaatt ctgcaaccat tggatcatct
     8281 tcgattatca ggacatttgt catcattttg atcttccttt tttggtaatt ctagatagac
     8341 gattgtaccg ccatttgttt gtttggaaaa ttctaaaaat ccaccgtggc gtttgacgat
     8401 tgaatggatg agattcaatc caaacccacg attgattccc ttcgtcgaaa aacctagcgt
     8461 aaatattttt tctgagattt cttctgatat cccgacaccg ccatcgcaga cggtcacgat
     8521 gaatacttca gcttctgtgt catagtttaa atgaatccgg acctgtccgt ttctttcatg
     8581 tttttgtagc acggcttctt ttgcattatc aagtaagttc cctaacactt gcaaaagtga
     8641 atggatggag tcatccatct ccattttagg gataaaagaa tcgctttcta acagcatttc
     8701 aacagattgt tctttggctt cgtttatttt gcctaataaa aaaccggcaa ttgcaggtac
     8761 tttgatcaga cttgagactt ggccaatctc ttttttataa ttttgattta gctgctggat
     8821 aaaatgggtg acttcttcat acttcttcaa atcaatcaaa ccagaaatga catgaagttt
     8881 attcatgaat tcatgggttt gagctctcat ggagtttata tattgtttcg tccccccaag
     8941 ctcctccatt aatttcatta cttctgatcg atttcgtaaa gttgcgactg ctcctaaaaa
     9001 acggtcattg ctaaaaagag gtgtaacgct agaaatagtt tctatcccat ttaaatacat
     9061 ggattgagtc cgaacggtcg tttgatgctc aatcgtctct ttaaacaaaa cgtcatatag
     9121 attatcactg atttttttcc tacttcgaaa tccaatagct gctctgtttg gtagaaaatg
     9181 ttttttgcag gtgtattgat caagaggata gttttatcag aagagatggc taagattcct
     9241 tcatctactt cattttcgat aatcgttttt tcacttaact gttctgctat ttcttgtggt
     9301 tcaagtccaa gcagtacttt tttcattttt tgggcaagaa aaacggcgcc tatgatgcct
     9361 acgctaagtc caataatcaa tgaaaaaaca atcatttttt gtccatcttt gacttgattg
     9421 tcaatcgtgt tgtttgttaa gccaacacaa actgcaccaa taatctcttg atcataataa
     9481 atagggacga aatagcggaa gccgaaccct aacattcctt ctttttcaga gaaaaattct
     9541 tgtcctccaa tcactttttt cacatcttca tgacttgaga aaggaagacc gatgtaactt
     9601 gggtctgcgt gagaatatcg aatgccttca gggtccatga caacgataaa atctaaattc
     9661 gatgcaatca tgatttcatt tgttattcct tgaatcgcat gtgtcgggtc accagtctgc
     9721 agcttttttt ggatgagttt atcattggca acgattttag caatgctaga gattttatct
     9781 gacacactag atcggttcat cgaaagaata aaattcctga tcaatatggc agaaacaatc
     9841 aaagaaacga atgtggagaa acaaacgaca aggatgatcg tgttctggag ctttaatggt
     9901 tttttcttaa aaaaaggaaa cataggctac ctcgaaagaa tttattgtga aaattttaac
     9961 atgaaaacaa aaatttaaca aattgtcaac gataaacaca accttcctat aaaaaacacc
    10021 actgagtcgg taaaagaagc tatacagaat tttcaaacat tggtatgata gacgtagaaa
    10081 tggaaggagg cgtgtatatg atagggggaa tcgcacttga gacgatttat tggtatacat
    10141 tgctgatttg tgcaggattg gcagtcctat tgattatttt tggagatgta ttcgattttg
    10201 atggcccgct tgatccgatg cttattatcc cttggttaac tttcacaagc ttatttggtt
    10261 ttctaggtga gcgtttaaca gatcaatcat ctttattttt atttattgct agtggaggca
    10321 tatcgatagt gttggtattt ttgcttaatt tctatatatt gatgccgatg aaacatgccg
    10381 aatcaacgat gtcgtcttct gaaaaaactc tggaaggaca agtagctgtt gtgatcacac
    10441 cgatccctgt acgaggtatg ggagaaattc agttgaaaag tgttaccggt tccattagtc
    10501 gtccagcttg tttttacagc ccccaagaac agacagctga gcggggaacg aaagtattga
    10561 tcattgagat caaggaaaga atctgttatg tcatgcctta tgaaagcatg ataaaagctt
    10621 aaaggagcgt gtgttaaatg gaaataccag ttgtttttat cgcgatcgca atcgcagctt
    10681 tgattttact gatgttgctg atcgtctttg tagcgaagta ccagacagca aaaccggatg
    10741 aagcgttgat catcagtgga agttatttag gaagcaaaaa tgtgcacatt gatgaaagca
    10801 ataacaaaat caaaattgtg cgtggaggtg gcgcattcgt cttgccagtt tttcaacgtt
    10861 cgaatcggat cagtctatta tcgagtaaat tggacgtctc cacccctgaa gtgtataccg
    10921 aacaaggcgt accggtgatg tgtgatggta cttcgatcat caaaatcggt tcatctgtag
    10981 aagagattgc tacagcagca gaacagtttt taggcaagac aagagaagaa ctggaaaatg
    11041 aagcaagaga agtattagaa ggacacttgc gctcgattct tggttctatg actgtagaag
    11101 aaatctatca aaatcgcgat aagttcagtc aaagcgtaca agaagtcgct tctgtagacc
    11161 ttgcaaaaat ggggttgatc atcgtttctt tcactatcaa agaggtcaga gacaaaaacg
    11221 gttatcttga ctctttaggg aaaccaagaa tcgcccaagt caaaagagat gcagatatag
    11281 cagaagcgga agctctaaaa gaaacgcgta tcaaaaaagc tcaagcagaa aaagaatcac
    11341 aagcagctga attacagcga caaacagaga tcgcagaatc attaaaagaa aaagaactga
    11401 aattagcaac ctacaaacaa gaacaagatg ttgctaaagc aaaagcagac caagcctaca
    11461 atttagaaag tgcccgagca caacaacaag taatcgaaca ggaaatgcag attaaaatcg
    11521 tagaacgcca aaaacaaatc gaactggaag aaaaagaaat cacacgtcgt gaaaagcaat
    11581 atgattcgga agtgaagaaa aaagcagatg ccgatcggta cgctaaagaa caagaagcac
    11641 aagctcaaaa agtcaaagaa gtgactgaag ccgaagcaga acgattccgt gtggaagcac
    11701 tggcagaagc ggaagccaat aagacacgtt tagctggaca agcagaagcc gaagcagcac
    11761 tagcaaaagg aaaagcagaa gccgaagcaa aacaaaagat cgccaatgcc ttcaaggaat
    11821 atggcgaagc agctgtcttg agtatggtta tcgatatgct accgcaattg atgcgcgaag
    11881 cagcacagcc gctaggaaat atcgaaaaaa tctctgttgt cgatacagga agcagttcag
    11941 gagaaaccag tggagcgaac cgagtgacaa attatgccac aaatttgctt tcttctactc
    12001 aagaaacact aaaagcgaca acaggacttg acttgaagga aatcgtagag aatttgtcga
    12061 cacgtgggac tactagccaa gtaaatatgt atgaagcgcc aaaagaggca gaacaaaact
    12121 cgatgaagga agaataaagt agtacacttc accgttcaat caaaaatgga ggaagacttt
    12181 ttaaagttct tctctccatt tttttatttt attgcaaaag aaaattagtc ctcgctattt
    12241 ttcttttgtt tttggatctt agcttctatc tcttgtaaat ttgttcttag caatcgttct
    12301 ttcttttgga gttcgaccaa atcttctgat agttcttcct tcttttctgt ttcaggagga
    12361 cgaagaaaga aattgatacc gattgcaatg aatgtcccta taaaagtatc tagtacacgt
    12421 tggatagcat acaaagcgga ttcgccttga gggatgctca acgcaatcag caacaacgta
    12481 gctattgcgg aaatgatacc ggaattattg ttaataccat ctgacacgac gatcacaata
    12541 ataactagaa taggaagcaa gatcaattct accaaaaaat cattgtgaaa atagcttttg
    12601 acaaaaaaat aaatgatagc taatgtacca cctaatgtat tgccgatgat cctagacttt
    12661 ccaaaagaaa cggttgtagt caagtcctga cgcaaagaaa aaacagcaga taatgcagca
    12721 atcatcggga ttcctcgttg agtcaaatga aaaaacaaga tacataaaaa aacagctaag
    12781 gcacttttga tcgtacgcat acctagtcga aaacggccaa attgcataga gattctcctt
    12841 tttggtcttt ttactatcat acctaaactt ttgtctgata gctagttttt cattattcaa
    12901 gtataattaa aataaaaaag ggggaagtaa atgcgtatag gaatgcgaac agtcaaaaca
    12961 gtgatcagtg tcttcttttc gatggtcaca gcgggtagct tatcgctgtt gtattggcca
    13021 gctgcaggga tcattgcatt attaagtgta ggaaatacca agcgctctac attgcgtacg
    13081 ggcatgaatc gaatcgcagc ctttatatta gctacggcaa cagcactgtt cagttttttt
    13141 gctgttgggt atactatttt tggcttcagt ctttttttat tcctgttcat cccgcttgcc
    13201 tctcgcttca aattaaccga agggatagta gtaaattcgg ttttggtcac tcattattta
    13261 gtggaggaaa acatgtcttg gcagctgatc ggcaatgaag cgggattaat ggcgattgga
    13321 ttagtatttg ctttgcttgc aaatgtttac atgccagatc gtacgaagca gctgaaggaa
    13381 aaccaaatac agatcgaaaa agaatttcga aatattttga gacaaatggc agaatttctt
    13441 ttatcagaaa ataaagaaga tgtgcagatc aaatgcgaac atttactgac attcattcgc
    13501 gaaagccaag aagatgccag agaacatcaa gaaaactact ggctcagaca gccgctatac
    13561 tacgaaactt acttctctat gagaagagca caggccaatg tgattaaaga catgctggaa
    13621 aatctagaga gaatccagca accagcttat tatggaaaac atatctatgg tttattgatt
    13681 tatactgcag aaacattttc agagtcaaat gatggcagac aaattttaac acgtattgaa
    13741 gaagtttatg tattgtatcg gcaaatgcct ctgccgacta gtcgccctga attcgaggat
    13801 cgagcagaat tgttccagtt tttgcagtct tttaaaagtt ttattgaaat caaggcagaa
    13861 ttctcccaac aaaaaatcca aaaatagagg ttgtgaattc agctaatatt caggtcaaaa
    13921 agctgatttc acaacctctt ctttaaacgg tgttctcctg tcaactcctc ggtcacaagt
    13981 cacaatattc aacaaacatg agaagctgtt ttttgaaatt gctccttgtg actcggagct
    14041 gaacggtagg ctcacaacct ctttcttaaa cggtgttcaa aagctttcct ctgcggtaat
    14101 aagtccagcc aaacgaaaaa taggaggagc tatttttgtt tggctgttct tattacttga
    14161 tgcagaacag ctttttcaca accttttaaa attacatatt tttccataga acttgcattt
    14221 gatccaagat ttcgatgaca tcttttgtca agaagagaga gcgattgggc aagtcacctt
    14281 caatcatgtg taccatattc tttatttcgt aattcattgc ttcagatgta ttcccgcttt
    14341 cgatgaattc ctttgtacca tctgtaaata agatctccgc acgatcagca cgcgggtaat
    14401 cagcaatcgt cacataaccg ttctcaaagg caacgatacc gacttttggc atttttgcct
    14461 gaaaagtcaa gctgacagca gcaagttcat tttctttgtt acgcaagata gtgacggatt
    14521 gttcgtccac gcctgtttca aaaggaacca ttgtagaggc aactacttca ggttgagagc
    14581 tcaaaaagaa acgagcgaaa gatacagcat acgtaccgat atccaacaga gctcctccag
    14641 ctaattctgg attgaagaac cggtttttag ggtctggttc tttgtaactg ccgaaaggtg
    14701 cttggatcat ttttaaagcc cccagttttc ctgtatccat tattgagcgt aactgctgat
    14761 aaagtggcat gttgaagatt gtcatcgctt ctgctaagat caaattcttt tcttctgcta
    14821 agcgcatagc gtcagccagt tcttttttat tcatcgtgat ggctttttca catagaacgt
    14881 gtttgcctgc ttccaatgct gccaagatat ggtcgatatg ctgtctattt ggtacagcaa
    14941 tgtacacgat gtcgatttca gcatcgtcta acatttcttg atagcttccg taagcttttg
    15001 gaatattgta acggtgagcg aagttttccg ctttttctga agttctggaa gcaactgctg
    15061 ttagttcgct cgtctcttga ttgaagcttt cggcaaactc atgtgcgatg gtaccaagac
    15121 caacgattcc ccattgatat tttttcatct gaaaacgctc cttcatagtt ggattgagtt
    15181 tcttgtctgt ctattattat aacaaaaatc acgaagaata aagaggagag gacttgaaac
    15241 attaaaaaaa atgaaagcag cgatttaaga ggtgcttttt gatcaaaact taagtagaat
    15301 agttaaaagg aggggataaa cgtgcatcaa gtatatattg atcttattat gagtgtatta
    15361 ttagaacagt ttgaaactga aaaaaatttt ttagatgaat acttgctgat cagtccagaa
    15421 caatggcaaa aatggaaaga tggcgagaaa aatctgccat ctgaagcgat gcagaaagta
    15481 aaaaatcttt tcactgatta tgaatggatg ctaacgcaaa agattttgcg tcaaacgatt
    15541 atttttcccg aaaaacgaaa cgttgccgtt gcagagtaca agcaaatcaa aacacgcatc
    15601 gcgcaaaaat ggcttagcag caaagcagca aaagtcgagc tgatcccttt gaaaaataat
    15661 cagcaggctg gtagatacct tgacttgaaa gtaagtattc aatatgatga atggggctat
    15721 gatgatattt tgaattttcg tctgccagca gatgttcaaa aacagatcga aggagaaaaa
    15781 atcgaacttc tcgattgggt gaatgaaaat ctggaagaaa cttacgttca tgaggcgaat
    15841 ggggaacgtg aagaacaacg aaaggatttt tcaagtgaat aaatttttag gatttggagc
    15901 agttgtgatt atgatcgcag caatgggcta tgttattttt agcgatgatg attcgcatgt
    15961 tcaagccggc aatcaagcag tcgaacgaac aagcaccaaa acaactgcta gtaaagaaaa
    16021 gaacagtcaa acaaccagca gcaaggtaaa ggacaaagaa acaaaagatc gtacgtccaa
    16081 acaattgcca gatgttaaaa cagacgattg gaagctagtt ttggttggtc cagatcacaa
    16141 attagaaaaa gaaattgatg agtctactca attggcatca ttaagtaacg gatacttgat
    16201 cgacaaacgc attgctccag aatatgaagc atttgccaag gctgctgaag cagctggatt
    16261 tccattagtg atggtctcag cttaccggtc tgtatcatcc caacaacaag ttttcgaaca
    16321 aaatgtccaa gatgtgatga gtcgtcaagg tgtatcagaa gaggaggcga caaagattac
    16381 taaagaaacg attacggagc caggatacag cgaacaccac acaggtctgg cagtcgatgt
    16441 cgtcgatcaa aactggtaca acagttatcc ttctactgta ttggatgcaa gttacggaga
    16501 tcagcctgga gctaaatgga tcgcagataa tgctgcgcag tatggattta tcgttcgtta
    16561 tccaaaagga cacgaagata taactaagat cacatatgaa ccttggcact tacgttatgt
    16621 cggtaaagaa aacgctgagt atatcaccaa acaccaattg acgctagaag aatatctgaa
    16681 tgaattaaat gaaaagtagg aattgatcat ggcgaaaaaa aagtggaaaa agcaaaagcg
    16741 caagaatatc cgttggccag cagtggttgc cggtgtagca gtgatactct tagcctttgt
    16801 tttttccttg aagaatctgt cttctccttt tactgataat cagcaagaaa atgaagcgca
    16861 atcccatcag caatttattg aacggcttgt tccgcatgcc caagaactac aagatggtta
    16921 tggagtgctt cctagtatca ttttaggaca agctatatta gaatcaaatt ggggaaaaag
    16981 taccttggct agcaaataca ataatctttt cgggatcaag gcttatgggg atcagaagaa
    17041 agtaagcctg gaaacaaagg aatttgttaa cgaagaatgg ataactatcc aaggagactt
    17101 caaagtgtat gattcatggg aacagtccat ggatgaccat acacaactgt tcgtccaagg
    17161 agtagattgg aatcctgcgc tgtatgaaaa agtgattacg gccaccaact atcaggaagc
    17221 cgcacaagca ttgcaagatg caggttatgc gacagacccc ggttatgcgc aaaaaatcat
    17281 ccaggtcatc gagacgtatc aattgaatca gtatgaccgg agatagcaaa ggagaagtgg
    17341 ctgatggcag aacaatttac gccagaaatc atgacggaat tgagaaaaga tatcgtaaga
    17401 gtaccggaaa tcattttaga agcaagcggg atcaagatat ttggaaaatt gatccgttcg
    17461 atcatcttta ctacggatat tgcgatcata cgtaatacaa atgcgaatgc agtaattgct
    17521 gtgtatcctt ttacacctca tccagcgatt actaaaagca ttattgaggc agctgacatt
    17581 ccagttttct ccggtgtagg tggtggtttg acacaaggtg ctcgttcttc ctacatgagc
    17641 atgttcgcag aagcgcaagg ttcgttaggg gtcgttttga acggtcctac gcctttagag
    17701 acagtaaaag aagtttgtcg agtagttgat attccagtta tcagtacggt aacgtcgaaa
    17761 tatacagaaa tagatgaaaa gttagcagct ggtatttcga ttatcaatat cagtgcaggt
    17821 aaagaaactg cagaagcagt acgccatttt cgtaaaaaat atcctgaact tcctatcatt
    17881 gcgacaggtg gtccaacgga agaaagtatt gctgagacca ttcaagcagg agcaaatgcc
    17941 atcacatata cgccaccaag tagcggagag ctttttagtc gtaaaatgga aaaatatcgg
    18001 gaaactgaga aaaagaatca tgaataacag gtgagaatca aaagacgagg aagagcagat
    18061 agtggctctt cctcgtcttt tgtactccag tactatttgt tcagaattca aaaaaaagag
    18121 aaattttttt gtttttttac ctgtaatgta cataagaaga gttaaaacct tttgttatta
    18181 ttagcattct aacaaaaaaa tgtcttgatt tattgaagcg ctttctatag aatgaaaaac
    18241 gaataaaaac taaaaaaggc acccgacaat ttagtagcag ctaaattgtc ggggcttgaa
    18301 aagtcagcgg gaactgacca atcaagttgc catagtcaat gcttgtgcat cgacgtgtat
    18361 attgtaccta atttcataca gaattactag ttatttttct gttttttagg gaagagcgca
    18421 cacgaagaag catgcttatt tagctatgtc aaggatattg ggcagttgtc tgatatcctt
    18481 ttttctttgc gtaattatcc tcccatcatt gaagcaagaa gtgtttttag cgatgaaaga
    18541 agaatcagca ctatctaatt tttcttgaaa gtattgagtt gttcatccta ggcagaacga
    18601 taaggcaagt cttggttttt attccccttg aaaaagatcg tttaataaat atgttctgta
    18661 taaaaataca gtatattgaa aaataacaat ggaagtagag gtgcagagaa tgaagaaaag
    18721 aatgagaatc atcttagctt taagtgttat gttattcttc ttgacggctt gtgggcaaga
    18781 agatgacaaa aaaatcacgc tgaacatggc ttggtggggt tcgcaagtcc gacacgatgc
    18841 aacagtaaaa gtaatcgagc tttatgaaga aaaaaatccc catgtgacga tcgattatga
    18901 attttatgat tatgaaggat attacacaaa attaggtaca ctagcagcat cagatgatat
    18961 ctgggacatt ttccaactga acaatcaatt tcctcaatac atcaatcaga ttgaaccatt
    19021 agatgaatat gtggagcaag ggataattga tatttctaat gcagatgaat cctatttagc
    19081 aacgacgact tacgacgggc aattagttgg actgtctaac ggggtgaatt cgtttgcgat
    19141 tgcttacaat aaaaatatat ttgaacaatt aaatatccct gaaccagctt caaattggac
    19201 atgggatgaa tttgaacaaa tcagctatca aatcactgaa gaattaggaa tctatgctat
    19261 ttcaagattc gaagatttta tagctggctg tattattcaa ataccacaag ttgagaaagg
    19321 attgaatttt tataatcggg aagatctatc taacagcttg ggatttgaaa atccagacta
    19381 tctaacagat tttttccagc tgcgtaaaaa tctggtagac aaaggggccc atccagaacc
    19441 aggtggtgga gctgcaagta cgacggatct gtcttttcag gcggttaggg atagagaagc
    19501 ggcaatggtg tttctatcaa gtaaccagtt gacagaaata ttgtcagggg ctcctgaaga
    19561 tatggagatt cagttgatta atccacctcg cagaaaagca gatggagaat ctggtatccc
    19621 tttacgttct tcgcaaatgc tgagtatggc aaaaagctca gcccataaag aagaagctgc
    19681 taagtttatt gatttctttc aaaacagtga agaagccaat gaaattttaa aaggagaacg
    19741 tggtgtacca atcatgtcga atattcgaga acagctcgct cgtgcagatg atgcaacttt
    19801 gcaagcgaca tttgattatt tagaaatgat ttccgatatg gacaacggcg aagtgaatgt
    19861 ctttgatagt gctttgaatc cggaaattca agaccaatac aatcttttag ttgaaaaagt
    19921 catttatgaa gaagtaacag cagaaggaag gcatgaaaaa agaaaaacta aaacggttct
    19981 tggatcaaga caatgtggtc ggttacgtat ttgcttctcc attcatccta ggatttctat
    20041 tgataacagt gataccgatg atcttgtcac tttattattc gttcaccgat tatcgtttgg
    20101 gaaatccagt ctcatggatc ggcttggata attatataaa gatgctgagt gatgcccgtt
    20161 ttttgaactc catccgagtc acttttctct atgtatttgc atctgtacct gtgaaactga
    20221 tctttggtct attgattgct tatcttttaa cgaggaatat caaaggaacg actttctttc
    20281 gttctttgta ttatgtgcct agtttgatcg gtggcagtat ttctatcgct ctagtttggc
    20341 gggagttgtt ttctaaaaac gggttagtga atattttcct ggaacgatta ggcgcacctg
    20401 cgctttcctg gtttagcgat caaaaactgg tcatgatccc tcttgttttg atgtctgctt
    20461 ggcaattcgg ttcggcaatg atcatctttg ctgcaggtat caaagagatc ccaggctcgt
    20521 attatgaagc ggcagagatc gatggagcga acaaatggca gacgttttac aagatcacgt
    20581 tacctcttct ttcgccgatc attttgtata acttggttat gcagacaatc gcggcgttta
    20641 tgacttttac gcaggcattt atcattaccc aaggtggtcc gaatgatgcc acgaactttt
    20701 atgctcttta tgtgtataac caagcttttt cttttaattc tatggggtat gctagtgcta
    20761 tttcgtggat catgctgttg attatgggag ctgtcacctt tctgattttc aaatcatcaa
    20821 aaaaatgggt cgttgtgtga atgagaggag gaagaaatga ataatacagt aacgaaatgg
    20881 tttcaatacg gttttatttt gctattaggt ctagtcatga tttatccggt cttatggatg
    20941 atcgcaggtt cttttaaaac ggatcaagag attatgagtg gttcgctgaa tttgattcca
    21001 gagacttttc aatgggaaaa ttacgcaaaa ggatgggcgg ggtttgctgg aatttcattt
    21061 tttactttct ttaggaattc gtttgtcatc tctgcagttt cgacgatagg gacagttctg
    21121 agctcgactt gtattgctta cgcattatcg agaatcgatt tcaaaggaaa aagattctgg
    21181 ttcgtcgtca tgttagtaac gatgatgatt cctgcacaag tgattttgat tccgcagttt
    21241 attatttaca atcggctgaa tttggttggg acgtatgtgc cgctgatttt accacatttt
    21301 tttggacaag catttttcat ttaccagatc atgcaattca tggtcagcat ccccaaagag
    21361 ttagatgaat cagcgattat tgacggatgc agcaagtata gtgtctttac caaaattatt
    21421 tttcctttgc tgaaaccttc gatcatcacg acgatcatca tccagtttta ttggaaatgg
    21481 gacgacttca tgggaccatt gatttattta aacaagccga gatcttacac ggtatcgatt
    21541 gccatcaaat tatttgcaga cgctggatct agtacgactt acgcgagtat gtttgcgatg
    21601 tctacgttgt cgctgctacc ggtttttctg attttcttat tcttcaataa atatcttgtc
    21661 caggggatca gtacaagcgg tctgaagggc tgaatatgaa aaagccaata gaagagatgt
    21721 ttctttacaa gctcaactac tatgtctgtc ggattttttt aaccagtctt atcagtaatg
    21781 ctcttttcgc tttagttcta ggatgtttgt tcactttagg attagtgctg tctgctcgtg
    21841 ttgtctatct gatttatttt ttgatgatgt atccttctta ttatctatct gtccattact
    21901 tggcagaaaa aaaggtatgt tggaggaaag ggaaaatcaa acggatgtgt ttcatcatta
    21961 cgttgtttca gctgtttgtc attatttttg cagttgattg gtatttatta gtgggaaaga
    22021 ttggagcttg gtatctgatt ccttttctat tgtgtctgtt gctttttgta tcccatagtt
    22081 tttttagtac gctaattgat atctgcaaag aagataaagc agatagtctt gtttatttga
    22141 tcaaaaggaa tatctggctg ttgtcaaaaa acgtcaaggg gtttctgtgg tatttatgcc
    22201 ttttgtatgg actgttctgg tgtatacagc ctgatttttt actcactgca ttactgtatc
    22261 cagggctgcc atttgtcaca gtattttgct taaaagaaaa ggagaatgac tttaaatgaa
    22321 gaaaaaatat gtacaagtcg gacttggtgg gagagcgcgt tttttttatg aggcaattgc
    22381 ctctacgtat catcaaactt gtgaactagt cggtttttgt gatgtatctc aaacaaggat
    22441 ggattatgca aatcggatac ttgtgaacaa gtatggacag aaaaaagtaa agacatgtat
    22501 gccatcacaa tttgagcaac tactggatga cgtacggccg gctgaagtca ttattacgtc
    22561 agtagatcga acgcatcatg attatatcat tcgtgcaatg aaaaaaggat atgatgttat
    22621 ttgcgaaaaa cctctgacta tcgatgagga aaaagctcaa gaaataataa cggtccaaaa
    22681 acagacggga agaagcctgc gagtcacttt taattatcgt tatgcaccgt atcacacgaa
    22741 gataaaggaa ctgatagaaa gtgatgtgat tggtgatgtg ttttctatcc acttcgaatg
    22801 gctgttaaat acgcaacacg gggcagatta tttcagaaga tggcatcgaa ataagcacaa
    22861 tagcggcggg cttcttgttc ataaatctac ccatcatttt gatctagtga atttttggtt
    22921 gaacagccgt ccccaaaagg tatttgcatt cggtggatta aacttttatg ggatgcataa
    22981 tgcggagaaa cgcggagtag aaatatttta cagtcgatgc tatggaagcg aactagctaa
    23041 agctgatcca tttgctatag acctgtcagc caatgaggaa ttgcgagaaa tgtatttaga
    23101 agcagaaaaa gacagtggat atattcgtga ccgcagtgta ttttccgatg atatctctat
    23161 tgaagataca ctaggcgtaa cggtcaaata tgaaactggt gcgattttga cgtattcgct
    23221 aaacgcgtat atgccgtggg aaggattcaa tgtttctttc aatggcagca aaggacggat
    23281 cgaaatgaaa gtgatcgaaa agtcttacat caatgcaggt ggagataaaa aggatgaagg
    23341 agcagccgtt cttcagtcca tccatgtcta tccgatgttc ggtgagccat atgaggtaat
    23401 cgtcgaacaa gaagcgggcg gacatggcgg aggagatccg cgtttattag atgatctatt
    23461 tggtgaagaa aaagaagaag atccgtggaa tcgggcagca actcacgtag atggtatttt
    23521 gtcgattttg acaggaatcg cagcaaatca ttctattgct tcaggaaaag cagtagtgat
    23581 tgacgagctt gtcagcttaa aagaataaga atagatttac ggaaattact tcccgtagtt
    23641 tataaaatct tgccttattg ccgataggct gtcttttgaa cactatttat tcggaaaaag
    23701 gggctatgac atgctttttg tcatgccttc tttttatgtg ggctgctttt ttagatagga
    23761 gaaaataaaa ataactattt aaacccgaac aaaaaggaat aatgcattaa aaatcattag
    23821 attttacaaa aaaataagta aaccacgttg tcagtttcag aaagtagtgt tagactgttt
    23881 tcaatatttg aaacaacatt ttttattcaa atatggcaaa ctgccttcac gtgcaaaaca
    23941 gttgcaagaa aatgaattta cactattatt agttttacat acctacttat atatcgtcat
    24001 aatatggatg acagtttcta gccagtaccg taaatgctgg actataagta aaagattggc
    24061 tatttcaccc ttcttttctt atgataaagt ggagggtgtt tttctttaac agaaacatag
    24121 taggtttttg cacaaaagcg gttatttcgg tattaataaa tgataaagga gcaaataaag
    24181 tgaaagaatt agttgaacgt attcaaaaag acggacgtat attaggcgag ggcgtattaa
    24241 aagtcgacag cttcgtcact caccaagtag accctgtatt gatggaacaa atgggaaaac
    24301 ggtttgcaga agtatttaaa gaacaaggaa tcacgaaagt tgtgaccatt gaagcttctg
    24361 ggatcgcacc agctttattt gctgcaagag aattagatgt tccaatgatc ttcgctagaa
    24421 aagcaaaaag tttgacgatg gatgaagaat tattgacagc ttctgtttat tcttttacga
    24481 aacaagtgac aagtacgatt tctatttctc gtaaattttt atcttcagaa gacaaagtat
    24541 tgattatcga cgatttcttg gcaaacggac aagcggcaaa aggattgatc gaattatgcc
    24601 agcaagctgg agcgcaagta gaaggaatcg ggatcgtcat cgaaaagtct ttccaagatg
    24661 gacgtcagtt acttgaagaa atgggattga gagtcgtttc tcttgcgcgt attgcttcct
    24721 tgacgaatgg acaagttgag tttcttgagg aggacgcata atcgtggaaa aagaaacaca
    24781 aaatggaaaa gctgcggttt tagggctgca gcatctgcta gcgatgtatg caggagcagt
    24841 agcggtgcct cttttgatcg gtacaggact aggctttaat caagaacaga tgacttattt
    24901 gatttcaatt gatatcttta tgtgtggatt agcgacatta ttgcaattga cagttaaccg
    24961 atttttcgga attggcttgc ctgtcgtttt aggatgtgcg atccaagcag tagctccact
    25021 gatcttgatc ggaaccaatg aaggagtagg agctatctac ggatctatta tcgcttctgg
    25081 gatctttgtt gtgttagttt ctggtgtctt ttctaaaatt aaaaaactat tccctcctat
    25141 agtaacagga acggttatca ccgttattgg tttgactttg attcctgtag caatcgagaa
    25201 aatgggtgga ggtaatgcaa cagcagctga ttttggtgac aaaacaaatc tgctacttgc
    25261 atttgtgacg attctcctaa ttgtaggcgt gcaaatgctg gcaaaagggt tcattcgttc
    25321 aatcgcggta ttgattggtt tagtcggagg aagcatctta gctgctttca tgggaatgat
    25381 tgatctgagc gcgattggag atgcgccgtt gttccatgtt ccacaaccct tttattttgg
    25441 taaaccaact ttcgacgtat ggtcgatttt actaatgatc atcatttcca tggtcagcat
    25501 ggtagaatca acaggtgtat attttgcact tggtgacatt actgggaaaa atgttggaga
    25561 agaagaattg aaaaagggat atcgtgcaga aggacttgcc gttatcttag gcggtatttt
    25621 caatacattc ccttatactg gcttttctca aaatgtcgga ttggttcaac tttcgggaat
    25681 caaaacaaga aaacccatct atttctctgc attcttcttg attgttttag gattattccc
    25741 taaaatcggt gcgttagcac aaatcatccc tgagccggta cttggtggag gaatgttagt
    25801 catgttcggg atggtggcag tccaaggaat ccgtatgctg ttggaagttg atttcagcaa
    25861 tgataaaaat ctattgattg ttgctgtttc tgttggattt gggctgggtt tcaatgttac
    25921 gccgacatta ttcagtcaaa tgccgcaaac attgcaaatg ttcactggaa atggcatcgt
    25981 aatgagtagc attacggcta tcatattgaa tttgatcttt aatggattga aaaaagatga
    26041 agaaatattg gaagaagcct tcaagaagta atagagcaaa agaaaggttg tgaaatcagc
    26101 gttttgacct gattgttaga gaaacaaacg gaaaaatagt tcctcgtatt ttgccgtttg
    26161 tttgagctaa taccgagggt cagctgattg aacaccgttt atccataaaa aggaatgtga
    26221 cctaactttt gtcacattcc tttttttcat ctttaataaa cacgaacaat aaaatgtttt
    26281 tctttgttaa cattcgaaat ttctttgaca tcaaaaaaag tgattgttaa aatatgaatg
    26341 tagaaaaact gggaggaaaa acaatgacag caaaaatctc tgtgattatg gggagtactt
    26401 cagattggga aacaatgaaa gaagcatgtc aaatattgga tgaattcggt gtgtcttatg
    26461 aaaaaaaggt cgtttctgct catcgaaccc cagatttgat gtttgaatat gcacaaaacg
    26521 ctcgaaaaaa aggaatcaaa atcattattg caggagcagg gggagccgcg catctgccgg
    26581 gtatggtcgc atcaaaaaca actttgccgg ttatcggtgt accggtccaa tcacgaacat
    26641 taaatggttt ggattcgctt ctttcgattg tccaaatgcc tggaggagta cctgtagcta
    26701 ctacagcaat cggaaaagcg ggagcaatta atgctggact acttgcgata cagatgcttt
    26761 ccatgtatga tttagaattg gaggctaaat tggctgaaag acgttcaatg ttggctaaaa
    26821 ctgttataga aagtagtgat caacttggtt aaacctttgt tgcctggttc aacgatcggg
    26881 atcgtcggtg gcggtcaatt aggtcgtatg atggcgatta gtgcaaaaga aatgggcttt
    26941 aaagtaggtg tgctggatcc tgtgagcgat tgtccagccg cacaagttgc agattggcac
    27001 attgaaagtg tctatgatga cacctttgcg ctgaaagagc ttgccagaag aacagatgtc
    27061 attacctatg aattcgaaaa cgtcagtgta gaggcgttaa atgcgatttt acccatgtca
    27121 tttattcctc aagggacaga cttgcttgcg attacgcaag accgcctgtt agaaaaatct
    27181 tttttggaaa cgaataacat cgtgattgct ccttatgcaa cgatcgtcag tccgacggat
    27241 atccaagatg cgattgacgg gatcggctat ccttgtgttt tgaaaaccac tcgaggcggc
    27301 tatgatggca aggggcagta tgttttgaaa agtcgggcgg atctagctcc tgccatggac
    27361 cttttaagag aaggaacctg tgttttagaa gcatggatcc cgtttgaaaa agagatttcg
    27421 atcatggtgg caggaaatgg tcaaggagat ttcacgactt ttccggtcgt ggaaaacatc
    27481 caccacaaca atattcttca tgaaacgatt gctccagccg cgattgatca ggatgtgatc
    27541 gaggaggctg agcgtattgc ccgtgtgatt gcagaagcag tgagcctttc aggtgtttta
    27601 ggggtagaaa tgtttttaac aaaaacaggc gggctatatg tgaatgaatt agctcctcgt
    27661 cctcacaatt ctggacatta ttctattgaa gcttgttcta tgagtcagtt cgatgcccat
    27721 attcgaggta tttgtggttg gccattagga gaaaaagtac gtttgataag caatgcagta
    27781 atggtcaatg ttttgggaga acaactgctg gaaagctatc gcttgattga gaataagcca
    27841 gactggcaat tccatttcta tggaaaaaaa caagcaaaaa aaggtcgcaa gatgggacat
    27901 atcactatct tgacagaaga catccagcaa acgctggaag aaatcaaaca aacgaatatt
    27961 tgggattaga ggagaaaaaa catgttagaa cgttatacac gccctgaaat ggggaatatc
    28021 tggacggaag agaacaaata ccgtgcgtgg ctcgaagtcg agattttagc agatgaagct
    28081 tgggcagaac ttggtgaaat cccgcaagaa gatgtgaaaa aaattcgtga gcatgcatca
    28141 tttgatatcg atcggatctt ggaaatcgaa aaattaacac gccatgatgt cgttgcgttt
    28201 acacgagcgg tttctgaaac attgggagaa gaaagaaaat gggtccatta tggtctaaca
    28261 agtacggatg tcgtagatac tgcttatggc tatctcatca agcaagcaaa tgatatttta
    28321 cgggaagact tgaaacgttt tacggaaatc attggcgaaa aagcaaaaga acacaaatat
    28381 acagtgatga tgggtcggac acacggtgtc catgctgaac caactacttt tggattgaaa
    28441 cttgccacat ggtattcaga aatgaaacgg aacatcgaac gatttgaaca tgcggcaaaa
    28501 ggagtagaag caggaaaaat ctcaggagca gtgggaacgt tcgcaaatat cccgccattt
    28561 attgaagagt atgtatgtaa acaattgggg atccgtccac aagaaatctc tactcaagtt
    28621 ctccctcggg atctacatgc tgaatatttc tcagcgatgg cgcttattgc tacaagtatt
    28681 gaacgttttg caacagagat acgcggattg caaaaatccg aaacacgtga agtagaagaa
    28741 ttttttgcga aaggtcaaaa aggttcttct gctatgccgc acaaaaggaa tccgatcggt
    28801 tcagaaaata tggctggtct agccagagtg gtgcgcggac acatggtcac tgcctatgaa
    28861 aatgtcacat tatggcatga aagagatatt tctcattctt cagcagaacg aatcatcttg
    28921 cctgatacca cgattttatt gaattacatg ttaaatcgtt ttggcaatat cgttaaaaat
    28981 ctaacggtct ttcctgaaaa catgaagcgg aatatgtctg caacatttgg tttgatttat
    29041 agccagcgcg ttatgctgaa attgatcgat aagggaatga caagagaaga agcttatgat
    29101 cttgttcagc caaaaactgc gtacgcttgg gacaaccaag tgcagttccg cccgctgtta
    29161 gaagaagatc caaccatcac atcggtatta tctaaagaag aaatagacga tgcctttgat
    29221 tatcatcatc atttgaaaaa cgttgacgtg atattcaaac gagtaggttt ggaataaaga
    29281 agagtgagtt gcaaaattca attaaaatag aatagcaaaa ataaccaaaa aagaagcaga
    29341 tccgttagcg gttctgcttc ttttagttta ttatattttc ttatcaataa taggaacctg
    29401 attttaaaag cggctacctc tttaccgtgc ctctttacat acaaaaaata gaggaattaa
    29461 gcgtaaatgg cttggctatt ataactaaaa tgctggttat ggagatgttg cagcaagttt
    29521 tcgagaaaag cgtattccat ctcttgtcca ttgaataaga tctggatacg ataatgattt
    29581 ttgaaacgct tctgttcatg aatctgactg attgaaagtt cgcctttgtg ttctgtctga
    29641 taaagaaaag tccttttacg gcattcagca cagcgcttgg ttgctcggcg gatttttcgg
    29701 aatgtataaa aactagtatc tttattgcat aaataaacgt ctttgatcga cagcaaatca
    29761 ttatatagac aggaacgttc ttcagctgtt aattctgata aaaaaagctg accttgtttt
    29821 attgcatctt ccatcggcaa gtgcctcctc gaacccatta tccaaatttt tagttaattc
    29881 taatatacca tcattaagta taaatgtaaa cgcttgttga tggtataaga ttttcgtttc
    29941 tgataaaaca cgaacattaa aagaatctaa tgccttaaat gtacatattt ttgttgattt
    30001 ataaaaagat gattgctaaa atttaaagtg aaaaaacgaa caaaaaacga tgtatttgct
    30061 tttaatgtaa ataaaaagga gagacgtaca tggagaagaa agaacttttg tatgaaggaa
    30121 aagccaaaaa aatatatctt acttctgatg agaacttgat ttggatagaa tatttagatc
    30181 aagcaactgc gttgaatggc gcgaggaaag accagataag tggaaaagga gaattgaata
    30241 atcaaatcac aagtgcaatt ttccgttatc tgactcaaag aggaataaaa aatcatttta
    30301 ttgaacaatt gtctaaaacc gaacaattag caaaaaaagt acaaattatc cctctggaag
    30361 tggttatccg aaatattgca gcaggaagtt tctcaaaacg tctaggtgtt cccgaaggta
    30421 aagaattcat caaaccaatc attgaattct actataagga tgaccagttg gatgatccat
    30481 ttatcaatga agagcatatc tatttcttac aacttgcaaa accggaagaa atcagtgagc
    30541 tgaaagccca agcgttggct attaatgaaa ttttgaccga attatttgct aaggtcggtt
    30601 tggttttagt agatttcaag ctagaatttg gccggtcgaa tggacaaatt ttgcttgcgg
    30661 atgaagtaac accggatact tgccgattat gggatgaacg tacgaatgaa catatggaca
    30721 aagacgttta ccgcagagat ctaggtgatt tgatcccagt ctatcaagaa gtatataacc
    30781 gtctatcgga actaactgaa gaaaaggagc gagtatgatg tatttcgtaa aagtatatgt
    30841 aacctataaa gattcagtgt tagatcctca aggggaggca gtaaaaaaag cagtccatcg
    30901 tatgggatat gaaacaatcg aagatatccg aataggaaaa tactttgaga tacaacttgc
    30961 tgatgcagaa gaaccaatcg atacagtgat tgaaacgatt tgtgacaagc tgcttgcaaa
    31021 tgtaaatatg gaaacatacc gatatgaaat caaaaaaatg gaggaagtat aacatgaaat
    31081 ttgcggtcat cgtttttcca ggttcaaatt gtgacgcaga tatgttatgg gcagttcgcg
    31141 agatcatggg cgctgatgcc gaatttgtac ggcatgatga gtcctcttta gaaggatttg
    31201 atggggtttt actgccggga ggattctcct atggtgacta tttgagatgc ggtgcgattg
    31261 ctcggttttc tacaatcatg gaagaagtga ttcgttttgc tgaagaaggg aaaccagtat
    31321 tcggtacatg caatggattc cagatattga cagaagcagg acttttgcca ggtgtactga
    31381 gacgaaatga gtctttgcat tttatttgca aaactgtacc attgaaagtc aaccccaaga
    31441 cggcctttac ttcagagtat gaagccgacg aaataatcta tcttccagtt gctcatgggg
    31501 aaggaaatta tttctgtgac gaagaaacac ttcaagaact aaaaaaaaat aatcagattg
    31561 cctttactta taatgacaac atcaacggca gcgtggaaaa cattgcaggg attacgaaca
    31621 aagcagggaa tgtgttagga atgatgcctc atccagaacg tgcagtagaa gacttgctag
    31681 gatcagaaga tgggaaacgg ttctttgctt ccattttaaa aaacttcgga aaggtgaccg
    31741 tatagatgaa aacattgaaa gaaccgacag ctgaagagat caagaaaaaa agaatctatg
    31801 ccgaatgggg attgactgat gaagaatatc gaatgatcga agaagatatc ttaggaagaa
    31861 tgcctaatta tacggaaact ggactgtttt ctgttatgtg gagcgaacat tgttcctata
    31921 aaaattctaa accggttcta cgcaaatttc ctactagtgg acctcatgtg ttgcaaggcc
    31981 ctggagaagg cgcaggcatc gtcgacatcg gtgatggact agcagttgtc ttcaaagcag
    32041 aaagtcacaa tcatccttca gccgtggagc cttatgaagg agctgcgaca ggagttggcg
    32101 gaatcatcag agatattttc agcatggggg ctcgtccaat tgcgttgcta gattcattga
    32161 ggttcggcga attagataat ccaagaacga aatatttact agaagaagta gttgcaggta
    32221 tcggcggata cggcaattgt atcggcatac cgactgtagg cggcgaaatt gcatttgatc
    32281 cttgttatga aggaaaccca ttagtcaatg caatgtgcgt aggtttgatt gaacacaaac
    32341 atatccagaa agggcaagcc gctggcgtcg gtaattcgat catgtatgta ggtgcaaaaa
    32401 caggtcgcga cggtatccac ggagcaactt ttgcctcaga agaatttaat caagaagaag
    32461 aacagcaacg ttctgctgta caagtaggcg atccttttat ggaaaagctg ctgcttgaag
    32521 cgtgtttgga attaatcttg catcattcgg attctctaat aggtatacag gatatgggag
    32581 cagcaggact tgtttcatct agtgcggaaa tggcttcaaa agctggctca ggacttatat
    32641 tgacattaga tgaagtccca caaagagaaa ctgggatgac accttatgaa atgatgttat
    32701 cggaatcaca agaacggatg ttgatttgtg ttaaatcagg agaggaagaa aaaatccaag
    32761 aactatttca gaagtatgat ctagatgcag tcactatcgg taaagtaaca gacgacgggc
    32821 aatatcggct atatcatcaa ggagaagaag tagcaaatct gccagtcgat gcattagctg
    32881 aagatgcacc agtttatcat aaagaaatga aagaaccagc tcgaatcgta gaatttcagc
    32941 agttagctcc ttatcagcca gttatcgaag aacctggaca agtattgctt gatctgctgc
    33001 agcaaccgac gattgcttca aaacgttcaa tctacgaaac ttatgattca caagtacaaa
    33061 ccaatacagt tgtccgtcca ggaagcgatg ctgctgtgat gagggttaga gggacgaata
    33121 aagcgttagc tatgaccaca gactgcaacg cgcgctatct gtatttggat ccaaaaatcg
    33181 gaggacaaat agcagtggca gaagctgcta gaaatattat tgctagcggt ggtaagccct
    33241 tggcgatcac cgactgcctg aactatggtt ctcctgataa accagaagtt ttttgggaat
    33301 tatcgacttc agctgacgga attgctgctg cgtgtgaaac gttaggaact cctgttattt
    33361 caggaaatgt ctctttatat aatgaaacgg atggtcaagc aatctatcca acaccaatga
    33421 ttggtatggt cggattgatt gaggatcata aacatatcac tactcaggaa ttcaaaaaat
    33481 caggggatct aatctatata ttaggtaaga cgtttgcaga ttttgatggc agtgaattac
    33541 aaaagatgca gcttggaaga atcgaaggtg tgatccgaaa ctttgatcta tccattgaaa
    33601 agagaaacca agagttagta ttgacagcca tccaaaacgg attgattgaa agtgctcatg
    33661 actgttcaga aggtggcttg gcaattgcac tggcagaatc agctttcaaa catcagctag
    33721 ggatatctgt acagtttgaa ttgtcctctg ctcaattatt tgcagaaact caatcaagat
    33781 ttgtgttgac agtcgcaccg gaaaataaga cacgattcga agaaatgatg ggagatgctg
    33841 ctgttttagc aggtaaggtg acggatgaag cgataatcga aatttcagca actgacggtc
    33901 agatcaaaat agaaactgca gtagcaagaa agtgctggga ggatgcaatc gtatgtctta
    33961 tgaagtgaag agtttaaatg aagaatgcgg gatcttcggt gtttggggtc atccagacgc
    34021 cgctagtgtg gcatacttcg ggctccatag tcttcagcat cgaggacaag aaggtgcagg
    34081 tatcgtgtcc aatgatcgag gaaagcttaa ggggcatcga gatttggggc tgctttctga
    34141 ggtattcaaa gatcaaagaa aattgcagca gttaacagga gaagctgcaa tcggacatgt
    34201 acgttatgca acagcaggca atggaagtgt ggacaatatt cagccttttc tattcaaatt
    34261 tcatgatcag tcgatcggct tagcgcataa tggtaacttg atcaatgctc gttcccttag
    34321 aaaagagtta gaggaaaatg gggcgatctt ccattctaac tccgatacgg agatattgat
    34381 gcatttgatc cgacgaagtg aagaaacaga ttttttaaat aaattaaaaa gtgctttgaa
    34441 tgaagtcaaa ggtggctttg cctatttatt aatgacagaa acggcaatga ttgccgcttt
    34501 ggacccaaat ggttttcgtc cattagcaat cggacaaatg aaaaacggag catacgtgat
    34561 tgcctcggaa acttgtgcat tagaagttat tggggcacgt tttataagag atgtccagcc
    34621 tggtgagata gtgatcatca atgacgaagg aatccagatt gattgcttta cgaaggacac
    34681 gcagctttct atttgttcga tggaatatat ctattttgcc agaccagatt caaacatcgc
    34741 agggatcaat gtgcatactg cacggaaaaa tatgggacgg aatttagcga aagaatcgcc
    34801 agtaaaagca gatatggtcg ttggcgtacc taattcttct ttgtcagccg caagcggtta
    34861 tgcggaagct agtggaatcc cttatgaaat cggattagta aaaaatcaat acattgcccg
    34921 aacatttatc cagccaacgc ctgagctaag agagcaaggt gttcgaatga aattatctgc
    34981 tgtacgagga gtagtggaag gtaaaaaggt gattatggtc gatgattcaa ttgttcgggg
    35041 tacgacaagc agacggatcg tccatctttt gaaagaagca ggggcaaaag aagtccatgt
    35101 gaggattgct tctcctcctt tgaaatatcc atgtttttac gggattgata ttcaaacgcg
    35161 taaagaactc attgcggcaa atcacacgat tgatgaaatc aaagaatgta tcggtgcaga
    35221 ttctttaggt tttttaagtg aagagggtct gatcgaatcg atcggtctgg aaaaagatgc
    35281 tccgtattca ggattgtgta tggcttattt caacggagat tatccgacgc cgttatatga
    35341 ctatgaagaa aaatatcagg aatcactaaa agaaaaagta tcgttctttt aaaagcagac
    35401 caagaaggga gtatatgaat tcaatggcaa atgcatacgc aaaagctggc gtagatgtcg
    35461 aagctggata tgaagtagta gaacggatca agaagcatgt caaaaaaacg gaacgtttag
    35521 gtatgatggg cgctatagga ggattcggcg gttgttttga tttatcggtc tttcaattaa
    35581 aagagccagt ccttgtctcg gggaccgatg gcgtggggac gaaattagca gtggcaatcg
    35641 aagcagacaa acacgataca atcgggatcg actgcgtagc gatgtgtgtc aatgatatct
    35701 tagcacaagg cgcagagccg ttatatttct tagactactt agcaacgggg aaaaatgatc
    35761 cgttacgttt ggaaaatgta gtaaaaggtg tagcagatgg atgtgttcaa ggaaatatgg
    35821 cgctgattgg cggagaaaca gctgaaatgc ctggtatgta ctcaggtaat gattatgatc
    35881 tagccggatt tgctgtaggg atagctgaaa aatctcaatt attgacagga gaaacgatca
    35941 acccaggaga tgtgttgctt ggaatagcat ctagcgggat tcattcaaat gggtactctc
    36001 ttgttcgcaa aattttcttt gagcagcagc agttttcttt atcatcaaaa ctgccagaat
    36061 tgaaaaaaac acttggcgaa gaattgcttg agccaacgaa aatctatgtt tcttccttgc
    36121 ttccgctgat caaagaaaag aaaataaaag gagctgcaca tatcactggt ggagggtttg
    36181 tagaaaatat cccgcgaatg ttaccagaat ctatggctgc acaaatcaaa cttggctcat
    36241 ggccgattct tccaatattc ggctgtttac aaaaatatgg tgaaattcct gagatggaga
    36301 tgtatgaaat cttcaatatg ggcatcggta tggtcctggc agtcgaagaa tcgaaagtaa
    36361 aagaaatagt acacgaaatt gagcaagctg gcgaaaaagt ttatgaaatc gggaaagtga
    36421 tcgccaaaga aaaacatcgt gtggtgttta taggagaaga acgatgagaa tcgctgtttt
    36481 tgcttctgga aacggaagca actttcaagc gctagccgat tatctatcga aaaaaggatt
    36541 ggaatcttcg attgactggt tgttttgtga tcagcctgaa gcatatgttt tgaaacgtgc
    36601 aactgcgttg agtgtaccgg cagattgttt ttcacctaaa gaatttgatt caaaaaaaga
    36661 gtatgaagaa gcaattttgc ataaattgaa agaaaaaaag atcgatttga tcgtattagc
    36721 tggatatatg cgaatcattg gaccggtatt gctgaaaaat tatgataaaa gaatcatcaa
    36781 tatccatccg tctcttttac ctgccttccc tggtttgcat ggcatacgtg atgcctttga
    36841 agctggagta aaagaaacgg gtgtcacaat tcattatatc gaccaaggtg tagatactgg
    36901 accaatcatc cggcaggaaa aagtgagaat tgaacaggag gacacgttcg attctttaga
    36961 agaaaaaatc catagagtag agcatcgaat ctatccagaa gtgattagtg aaattattga
    37021 aaatggaggc tatctgaaat gacaagagca ttgatcagtg tatcagataa aactgggatt
    37081 gtagcattcg cccaaggttt gattgaacaa ggagtagaaa tcatttcaac aggaggaaca
    37141 aagcatgcgt tggataaagc tgggatcaaa acgctttcga tcgaagaagt gacagggttt
    37201 cctgaaatga tggatggccg tgtcaaaaca cttcatccta aaatccatgg tggtctttta
    37261 ggaagacgcg atctccctag ccatatggag gcaatggaaa cggaaaatat ccaaccaatc
    37321 gactttgttt gtgtcaattt atatcctttc aaagaaacga ttatgaaacc agatgtcgaa
    37381 atagcagaag ctattgaaaa cattgatatc ggtggtccta gtatgctccg tagtgcagca
    37441 aaaaaccatg aatttgtcac ctctgttgtt gatccatctg actatgaaga agtactttca
    37501 gaattacgtc agtatgggca tacttcactt tctttcagac aaaaactagc ggcaaaagtc
    37561 tttcgtcata ctgcggcata tgatgcactg atcgctcaat acttaacgga ttgggtggaa
    37621 gaaaaagaac cggaaaaatt gacacttacg tatgaacgaa aacaaacgct tcgttacggt
    37681 gaaaatagcc atcaagccgc tgccttttat cagtccgctc ttctggaaaa atattcgatc
    37741 gcagcagcaa aacaactcca tgggaaagaa ctttcttata ataatatccg agatgcagat
    37801 gctgcgctac ggatcatgaa ggaatatgac gagccaacgg tagtcgcctt gaaacacatg
    37861 aatccttgtg gtattgggac agcatctacg attttagaag cgtgggaagc agcgtatgaa
    37921 gcagatcctg tttcaatctt tggtgggatc atcgttttga atcgagaagt agatctgcca
    37981 acagcagaac agatgcataa gctgtttcta gaaatcatca ttgccccttc ttatacacaa
    38041 gaagctttgg aattactgca aaaaaagaaa aacatccgat tactgacagt agactttgcg
    38101 caggaagaaa atcaaaatcg agaagaaacg gtctcagtct taggtggact tcttgtacaa
    38161 gatcaagatc tggcaataga aaatgaagaa gagtggaaag tcgtgacaaa acgtaagccg
    38221 actccagcag aagcaaaagc attagcgttt gcttggagag ctgtcaaaca tgtgaaatct
    38281 aatgcgatcg tactggcaaa cgaacgtcag acagtaggaa tcggagccgg gcagatgaat
    38341 cgagtcggtt cagtaaaaat tgcgattgaa caagcgcaag agaaaatgga aggtgcagtt
    38401 ttagctagcg atgcttattt tccaatggat gattcggttg aatatgcagc caaacatggt
    38461 ataaaagcaa tcattcagcc aggaggaagc atcaaagacc aagattctat tgatatggcg
    38521 aataaatacg atctagcaat ggtctttact gatgttcggc attttagaca ttagagagga
    38581 gtcggataaa tgaacatttt ggtgatcggt gcaggtggtc gtgaacatgc aataagtaaa
    38641 aaacttttag ccagtccgaa ggtggatacg gtgtattgtg cgccaggaaa tccggggatg
    38701 acaaaagatg gtatcaattg tgtcgctatc agtgagcgag atcattcagc gttaatccat
    38761 tttgttaaga aagaggctat tgattggagt atcgttggtc ctgaagttcc tttgttaaat
    38821 gggatcgtcg atgattttat ggcggaagga ttgaaaatct tcggtccgac aaaagcagca
    38881 gctctgatcg aaggttcgaa aagttttgct aaacaattaa tggatactta tcaaatcccg
    38941 acagctaaat ccagaagttt tgaatgttac gaaaaagcag cagcgtatat caaagaaaat
    39001 ggtgcgccaa tcgtagtgaa agcggacggc ttagcagctg ggaaaggtgt agtcgtggct
    39061 accacagtga aggaagcgct ggatgcggca gaacaaatgt tgaaccagca tcgatttggg
    39121 gatagtagca aaaaaatcgt cgtagaagag tttttggcag gagaagaatt ctcccttttg
    39181 gcgtttgtcc gtaataccaa ggtgtatccg atggtcattt cgcaagatca taagcgggca
    39241 tttgataacg ataaagggcc aaatacaggc ggaatgggtg cttattcacc agtaccgcaa
    39301 atttcaaaaa agatgattga aaaagctgtg gaagaagttt taaaaccagc ggctttggga
    39361 atgaaagaac taggacgctc tttcacgggt gttttatatg cgggtttgat tgctacgaag
    39421 gaaggaccaa aagtgatcga attcaatgcg cgtttcggtg atcctgaaac gcaagtcgtt
    39481 ctttctcggt tgaagagtga ctttgcgcaa gtaatcgatg atttattaga gaatcgtaca
    39541 gtagagttaa agtggcagca agaaggatac aatgtaggcg ttgtggttgc agctgccggt
    39601 taccccgaag aatatgagac aggaatggtt ctaccagatt ttctggaaga agagctttct
    39661 gtttactatg ctggagtttc ctcaaataac ggcaaactag ttggagcagg aggacgtctt
    39721 tatttagtag aagcatacgg tgagacgatc aaagaagcac agaaaaaaat ttacaccgcg
    39781 ttaatggata aaacgacaga tggaacattt tatcgtacag atattggaga aaaagctgcc
    39841 aatagcgaac tgatcgtgaa gtaaatcagc tgcttccatt tataaaaaat acaacaaaca
    39901 taatcggaag cgccgatttt agtgaagcct tttcataaag agtgtggtac acttttgttg
    39961 tgtatataaa taaggaggag ttcagatgaa aaaaattcaa ttaggtactt ctgatcagaa
    40021 agcagctgca gtgatcttag gctgtatgcg tatcaacaat gcggaagatc cagtaaaagt
    40081 gattgagaca gcttatgaaa acggcattga tttttttgac catgcagata tttatggaaa
    40141 cggtgaatgt gaagaaatct ttgcagatgc tctagcaaaa acatcaatca aacgagaaga
    40201 tctttttatt caaacaaaat gtggaatcgt tccaggtgtg atgtttgatt tttctaaaaa
    40261 acatatcatt gaatctgtag aaaaaagttt gacccgtctg aaaatggatt atgtggatgc
    40321 tttacttctt catcgtccag atacattggt agaaccagag gaagtagctg aagcgtttga
    40381 tgaattggaa cggtcaggaa aagttcgtta ttttggcgtc agcaatcaaa aaccaatgca
    40441 aatcgaattg ctgaaaaaat atgttcgtca accattagta gctaatcagc tgcaatttgg
    40501 cttgaaacat actggaatga tcgatcaagg gattcatgtc aatatgacag atgaagcaag
    40561 tatcgatcat gacggtagtg tacttgatta ttcccgattg catgatatga cgatccaagc
    40621 atggtcacca taccagtatg gattttttga aggtgtattt atcgggaacg aaaaattccc
    40681 tgaattaaac caaacattag aagaactagc agctaaatat caaacgacac caactggctt
    40741 ggcttctgct tggattttac gccatccagc caatatgcaa gtgatcgccg gtacgatgaa
    40801 cgtagggcga atcaaggaaa tcgctcaagc ttcagaaatt aatatgtctc gggaagattg
    40861 gtacgaattg taccgtgctg ctggaaacat tttaccttaa agattgtcat aagcaggtca
    40921 aaagggctat aaattaaaaa gacagaataa cttgaaagct gttttcagct tcgagatatt
    40981 ctgtcttttt ttattgcaaa tggaaagagt atcattgtac ggatttcgtt tattagttat
    41041 ttttgttttt aaggaaaagc tgtgtccgat attcttttgg agaaatccct tgttttttct
    41101 taaataaata actgaaatag ttagggtcat tataaccaat ttcaaaagca atatctgaaa
    41161 tacgatagtc tgattgctgc aataattttt ttgctaattc gattctttgt tctgtcagat
    41221 aatcgatgaa tgtttggtta aacgattgag aaaaaatcgt actgaaatga gagggactta
    41281 atgcaacttg ttgagcgacc caagttaagg acatatctgg atcggtaaaa ttttctttga
    41341 taaaatccaa tgcatggttc aaaattggct gatatttgtt ccttgaaggc tgtgagggca
    41401 gagcagttaa gaaagaaatg aacatataaa ctgtcgaacg gtaaagcgag tcgtcggcgc
    41461 tgattgctaa gcgttgactg gtggatgtgc aatgaaccat ttcttgcggg attttctttt
    41521 ctttcaggca gacatgctgg agtagcttag tcaagacaaa taaacgatat aatcgtgttc
    41581 gagttggtgt gccttgtggt ttcgtcaagt gttcgatgta cttagatact tcgtttttcg
    41641 ataatgaaga caaatcaagt gagaggtcaa agtaaaagac agaatcaact ggaagtttcg
    41701 cttcttgaat caaatcctca tagctgataa tttgttcttt aagggaactg gcggtttcaa
    41761 gtatcatctg cgtgatctca aaagatgcag gtaattcgct gattctttct gcgacaggac
    41821 caatggcgac agaaaaatta tttttttgtc ccttttctaa ttcatggacc aacgtttggg
    41881 ctacctgata actggtctcg atcacatttt cttcaagaga atcaactatt aaaaatttga
    41941 tgaatcgaga ggaaacagca gagaagatca cttgttgatt ctccctaaat aaaaacttca
    42001 aataatcact gagatgagcg taatcttcaa agtctgtact gacttggttt gctgctaata
    42061 atacggtcat ttttttccct gctaaagagc gattgaaaga ttcagcaagt ttcaatgttt
    42121 cactgatcgt aagtgagcct ttgaacaaac cgttcaagaa atgatttttt ttcaattcga
    42181 aaacaagatc agaagaagtg tgcgtttctt tttgctgcaa atctaaagca gttactgctt
    42241 tttctattgc ttgtttcaat tcgctacttt taatcggttt aagcagataa tcatctgcct
    42301 gtacctggat tgctcctttc atataatcga aatcgtcgaa tccactaata aatatgagac
    42361 gagtccaagg aagtagtttc cttgcttctt ttgaaaaagt caacccgtcc atgaaaggca
    42421 tttggatatc agtcagaatg atgtcgggct gtaagtcgat aattgaagac agtgccatct
    42481 caccatctcc agcttcgccg acataagtga ttggataata agaaccaagg acagttattt
    42541 gttcccgcaa gttattgcga atgaggtatt cgtcttcgat gataaaaact ttatacatgt
    42601 ttgtttcctc caattgtttt gggagataga gggatcatca gtgtgacagt tgttccttct
    42661 cggtatttgc tgtcaatcgt gataggtttt tgatcatttc cgtaataaag aaggagacgc
    42721 ttgtttacgt tgtaaagtcc gtagccgata ctgaaatctg aatcgatccc tttggctaac
    42781 tctttacgta tttctgccag tttttctgca gggatgccaa tcccgttgtc tataactttg
    42841 aattggatca tctcatcaat tttgcatgcg gtaatacgaa taaatccttt gtgacggacg
    42901 tacttcgttc catggtatac agcattttca atcaatggct gcaagatcat ttttaaaata
    42961 gaaacaggaa aaagtgtttc tggtatatcg atcttatagt caagcatatg gccgtatctt
    43021 atttttaaga tgactagata atcattgata tggctaattt ctttttcaag aggaatccag
    43081 tcttgtcctt tgcttaaaga cgtacgaaaa aagccggaaa gtgcatgggt catagtcaga
    43141 gcttcttcaa agcagtcttg ttcgatcaaa gaaataatgg catccaaact gttataaata
    43201 aaatggggag taatctgagc ttgcaataca cgaacttcac tttgtgctaa atggtattgt
    43261 ttcaaagcgt tctcccgtaa agactggttt agatcttccg ccattttatt catgctagcg
    43321 gtaagtgttt gtaattccag tgtttccggc agggttaaac gaaagccaag gttccctgaa
    43381 gaaagttgtt ctgacatctc gatcagctga ttgatcggtt cttggacctt tctctctaaa
    43441 aagcgacgat tctttttcgc atagaaaaaa atggtactaa gaactccaag ttccactatt
    43501 gttaatagaa tgagcgagcg gacaagtgtc gtattttttt tgcttgctaa gtcgatctcc
    43561 acacgaacaa aatcttgtaa gatatctgac agcagttgag tgacagaatc tacctgattg
    43621 atgatctctt cattggtatc cataaaatct tttgccaaga tattattgat cagctcgttt
    43681 tgataatctt ccagtgtatc gatcgtacgt agcgatacat ctaacgtact aatttcttta
    43741 agtgtatttg tattattttt gatctctcga atatcattcc taagttcttc taggatattt
    43801 tcctcattga actcatctac ggttacttgg ccagaaatca agtcccacat actttccaaa
    43861 actttttgat caaccttcaa agcaattgca tctgcttttt ggatatttgc gatcgtctgt
    43921 tgataaatca aaagctgttg cgaatataac agtatgctca gtagcaaagg gataacagca
    43981 aatagaagca tggctttgtt tgcttgggca aaaatctgtt ttatgcttaa attttttgtc
    44041 atgatacttc tccagtcaat aatttctggt tgggatgtta gctatattct gctgggaaaa
    44101 gacagtctcg gaaataaaaa tatctttagg taatgttttt cctgtttgaa tatttccaaa
    44161 ataacgagta acggtattgg ctacatacca gccgacatca ggtttacact cgactacaca
    44221 attgacgatt cctgagcgta gcttctcgat catatcttct tgtccatcaa tagttacaat
    44281 gatcaaatct tctccaggtt tgattgatgt ttcttttatc gcagctaaag caccgtgagt
    44341 catttcatca ctatggctgt ataatacatc gatttcggat aaatcatttg tttctaaata
    44401 tcgtttcatc tggtcttttc ctttcatccg aataaaatca ccgacaattc ggtgaacaat
    44461 agtcatcgga ggtttatggt cgatggttcc atcttttttt agggttggtt tacgattgat
    44521 tgattccaaa aatccttcgg agcgtagctt ggtcggtgtg gagttctctg aaccagcaag
    44581 ttctaggatt ctgatttcct gtttctttga atttgtgaag tgattgctga caaataaacc
    44641 cgcccgattt ccttctgctt tgaaactggg gccaatgtgg gtgacgaaaa gctcgggatc
    44701 attcgtttga atatgtctgt cgacaagtat gacagggatc cctgctcgct ttgcttcttt
    44761 taatacagag tcccacccgg attcttgcaa aggagtaaaa atgatcatat ccactttata
    44821 ggcaatgaac gtccgtatat cttggatttg ccgttcctga ttcatgtagc catttcggta
    44881 gagaacttgg taatcttctt tttcaagcgc agtgataatt gattcagtat gctttttccg
    44941 ccagctactc tccgttcctg attgcgaaaa accaacgact agtgggtcag gtgcttcctt
    45001 tgtattaggt gtacacccca tcatttggta agcgattaca acaagggcta aaaaaaataa
    45061 aacacgtttc actttaaatc actccatctg gatttgttag tactatctta tcagataaaa
    45121 gggaaaatca aagtcaaaag gaatcaatcg aaaaaaatcc aagaaactta aaaaaatcca
    45181 agacaaaaaa agtacaccta aaaaatttaa agtaaatccc ataaaaaaat caatgttaac
    45241 gctttcttta ttagctatac tcggtgtatc aattgaaatg ggggataaaa aatgaaaaac
    45301 aaatttagaa agattgttgg gactatcgct attttgaccg caagcattct attaagtgcg
    45361 tgtggcaatt caggatctgc agatgatagt acggggtatg taggaatcgc tatgccgacc
    45421 aaatcagcag aaagatggat cgctgatggg aacaacatgg tctcggaatt agaaaaactg
    45481 ggttataaaa cagatcttca atatggagaa gataaagtag aaaatcaagt agcacaaata
    45541 gaaaatatga ttacaaaagg ggtagataca cttgtgattg cttctatcga tggttcggct
    45601 ttaactgatg tgttagctaa agcaaaagaa gcagatatca aggtgatcgc ctatgatcgt
    45661 ttactgatga attcagaaaa tgtcgattat tacgcgactt ttgataattt tggagtaggt
    45721 gtatcgcaag cagcttatat tgaagagcac ttaggattga aagaaggaaa aggaccattc
    45781 acgattgaat tatttggtgg ctcgcctgac gacaacaacg cactaatcaa ttacaacgga
    45841 gtgatgtctg ttttacaacc atatatggac aacggtcagt tggtcgtgcc atctggacag
    45901 acgagtttca gtcaaatcgc tacgcttcgc tgggatggtt ctacggcgca agcaaggatg
    45961 gataatctgt taagcgcaaa ttatactgac caaacattgg atgctgtttt gtcgccatat
    46021 gatccaataa gtttaggaat catttcgtca ttaaaaggcg tggggtatgg gagtgaaagt
    46081 aaacctttac ctgtgattac tggacaagat gcgacagttg ctggcgttaa atcgatcatt
    46141 gccggggaac aaacacaaac gatctttaaa gacacacgta tcttagcaaa aaatacgatt
    46201 gaaatgatca aagcaatcag tgatggagaa gaggttccgg tcaatgatac tgaaacttat
    46261 gataatgggg tgaaaactgt ccctacgtat ttagcaaata cagtatctgt cgataaagat
    46321 aattatcaag cagagctgat cgacaccgat tattataaag aatccgatct gaaaaattaa
    46381 gaggaaccaa acaaggaact aaagagatct aagtcagaga gaggataaga attatggcgg
    46441 attgtatttt aaaaatggca gatatcacca aaaaattttc tggaatccga gcgttagatt
    46501 gcgtgaatct gatgattgaa cgtggggaaa tccatgcatt gtgcggagaa aacggagcgg
    46561 gaaaatcaac attgatgaat gtcttgagcg gcttgtatcc atatggctcc tatgacgggg
    46621 aaatctttta tgatggaaag ctgtgtcaat ttaaagattt gaaagatagt gaagaaaaag
    46681 gcatcgtcat cattcatcaa gaattagcac taagtcctta tctttcaata aaagagaata
    46741 tttttctcgg aaatgaacaa gcagaaagag gcgtgattga ttgggaactg acagaaaaga
    46801 aaacatatga actgctgaat aaagtcggtt tgtctatcga cccgaatctt ctagtatccc
    46861 agattggcgt agggcaacag caactagttg aaatagctaa agcattatca aaatccgtcc
    46921 gtctgttgat tttggatgag cctaccgctg cattgaatga agaagaaagc gcgaatttgc
    46981 tgagattgat ccgagagttg aaagagcaag gtgtaacctc catcattatt tcacataagc
    47041 tgaatgaaat cgtggatatc gcagatcgaa tcaccatttt acgggatgga cagacgattg
    47101 agacactaga aaatgaaatg atcagcgaag agcatattat tcgcgggatg gtgggcagag
    47161 atttagttga tcgttatcct gaaagaaaac cggatattgg agaagtttat tttaaagtag
    47221 aagactggac ggtctatcac cctcttgatc acgagcgaat cataaacgac cacatcaact
    47281 tgcatttgaa aaaaggagag attgtaggaa tagctggact gatgggagcg ggcagaactg
    47341 aattcgctat gagtctattt ggacattctt atggaagcaa gatatccgga cgtatattaa
    47401 aggaaggggc agagatatca gttaagaccg ttccttcagc cattgcaaac ggactcgctt
    47461 atgtctcgga ggaccgaaaa tctttaggac tgaatctgct gatggatatt cgggaaaacg
    47521 tgacgctgag cagtttggat aaaataagtc aaaaaggagt attagataaa gaaaaagaag
    47581 taactgaggc agaaaatttc cggaaaaaga tgcgtataaa aacaaactcc atccatcaaa
    47641 ttgtcagtag tcttagtggt gggaatcaac aaaaagtcgt tttatccaag tggctgatga
    47701 cacaacccga tattcttttt ttggatgagc caacccgtgg gatagatgtt ggggcaaaat
    47761 atgagatcta cacgattatc gaagaaatgg cagcaaatgg caaagctgtc tgtcttatct
    47821 cttctgaact tcctgaaata ttagggatgt gtgatcgaat ctatacgatg aacgaaggac
    47881 gtattacagg agaagtctcg cgagaagatg cagatcagga agtcttgatg aaattaatga
    47941 cgaaagaaga ggtgggttaa gttggaacag gcaaaaaatc ttacggataa aaaagagaaa
    48001 aaactaaatt tgaaaagtag agcagctgat ctttttggtc gctacagcat ggtgatcatt
    48061 ttagcgggac tacttattgc gtttcaattg atgacggatg gtattttctg gcgtccattg
    48121 aatataacta accttgttct gcaaaatagc catatattag tactagctgc agggatgtta
    48181 ttagttgtgc tcttaggtca tgtggatttg tcggtagggt cagtgatggc gtttgttggt
    48241 gcaattgcgg gcatgatgat ggtcaatcgc caaatgaacc cgtggatgac agttatcgca
    48301 tgtcttgcag taggcgggct gatcggcgca tggcaaggtt tttgggtagc ttatgcaggg
    48361 ataccggcgt ttatcgtgac tttagcagga ttgctgatgt ttcgtggact gacacaagtt
    48421 gttctaggag gccaatctct agcacctttt ccagctgttt tccaaaaaat gtctacgggt
    48481 tatttaccag acttaagcga aggaagcagc cttcacttct tgacattgat tttaggaatg
    48541 atcatcacga tcgtgttagt tgttggtcaa tggcgaacaa gaaatcgtcg gaaaaatcat
    48601 ttatttgaag tggaatctcc aacagctttc ttggtaaagg cggtatttac agcagtcgtt
    48661 gtactaggaa taacttatat ctttgcctct tatcaagggt atccgattat tttgatcatt
    48721 cttggtttga tcgttgcagc atatgggttt ttgacaaata aaacagtagc aggtcgacag
    48781 atttatgcga cgggcggaaa tcgaaaagcg gctgaactat cagggatcaa aacaaagcga
    48841 atcacgttct gggtattcgt caacatggga gtcatggcag ctttagctgg attagtttta
    48901 gctgctcgat tgaatgcggc aacacctcaa gcaggtacat cgatggaact ggatgcaatg
    48961 gcggcagtat attttggtgg tgcttcgact tccggcggca ttggaacaat catgggtgcg
    49021 attgtcggtg gcttggtcat gggtgtattg aacaatggca tgtcgatttt aggcgtcggg
    49081 gtagactggc agcaggcaat caaaggattg atcttgcttt tagccgttgt gctagacttg
    49141 tataataaga aaaaacgaac aacgtaggag aacgccggtt attcagaaaa aagactgtga
    49201 caagacattt gtcacagtct ttttttcgct aaaagcgaac attcattgtt tcgtttgtat
    49261 agactaaaaa taatatatgt ttttttacca ttcgtataaa ttctcttttt gctcttgatt
    49321 tttttgctta attgagttaa attacatagg gcgctaaaaa aagccttgat taaattaata
    49381 atgttcgtgt tttgctggat gtgaacatca ggaggataat gtaatgaaag tatttgatta
    49441 cgaagatatt caattaattc ctaataaatg tatcgtaaat agccgttctg aatgtgatac
    49501 aacggtcaca ttaggtaaac atacattcaa aatgccagta gtgcctgcca acatgcagac
    49561 gattattgat gaaacaatcg cagaattctt agcggaaaat ggttatttct atatcatgca
    49621 tcggtttgat gaagcagctc gaattccgtt tatcaaaaaa atgaaaaagc gtggattgat
    49681 tacctcgatc agtgttggcg tcaagaaaga ggaatattcc tttatagaga agttggcaga
    49741 agaatcgcta aatcctgatt atatcactat cgacatcgcg catggacatg ctaactcagt
    49801 tattgatatg attcagcata ttaaaaaata tttgcctgaa acttttgtga ttgccggtaa
    49861 tgttggtaca ccggaagccg tccgtgaatt ggaaaatgcg ggcgcagatg caacaaaagt
    49921 cgggattggt ccaggaaaag tatgtattac aaaaatcaag acaggctttg ggactggagg
    49981 ctggcaactg gcagctctta gatggtgtgc gaaagcagct cgcaagccga tcatcgctga
    50041 cggtgggatc agaacacatg gcgatattgc aaaatctgtc cgttttggtg cgacaatggt
    50101 catgattggt tcattgtttg ccggtcacga ggaatctcct ggcgaaacaa aagtcgaaaa
    50161 tggaatcgtg tataaagaat actttggttc tgcttcagaa ttccaaaaag gcgaaaaacg
    50221 aaatgttgaa ggaaagaaaa tctggatcca acacaaagga tctttgaaag atacactagt
    50281 ggaaatgcaa caagaccttc aatcatctat ttcttatgct ggaggaagag atctggaagc
    50341 cattcgaaaa gttgactatg tcatcgtgaa aaattcgatt tttaatggag acacaatcta
    50401 aaaaataaat aaaatattag gatgttgtga aaaagttgtc ctgcattaag taataagaac
    50461 agccaaacaa aaatagttct ttatatgttt tgtttggctg gaattattat cgcagataca
    50521 agcttttgaa catcattatt cagaataagg gactgtgata agatttttgt cacagtccct
    50581 tatgttttgc aaaaaaagtt tttctttttt ctcgtaaatt cggtaaaatc aaaagaaaaa
    50641 atagacaata gaaaagagaa gcttatgtgg gaaaaagtag agaaaaaaca aggaccgatt
    50701 tatcgtcaaa ttatggaaca gattatgaga gaaatcgaaa acggaaggat caatcctgga
    50761 agtcaacttg aatcagaacg aaaactaggg caactttttg gtgtgaatcg ttccaccgtt
    50821 gtccatgcat tggaagagct tagggagctt ggggtactaa cgagcaaacg tggcagcgga
    50881 tggtttgtta atcagactga atgggggaaa ttcgctgttc caagaatcga ttggcggcaa
    50941 atgatctctc ctcgttatga gcagacggac tggtatgagc aaaaaataag agaagcaaag
    51001 cagatccaaa aaacatcgtt tcttgattta tacgctagcg agatgccaga tgagcttttg
    51061 ccgaattttg agtttccttc ttattctctt gaagaaatct tagcggaaga aaaagaaatg
    51121 gatcttttag gctatactcc gttacgtaac aaaatcaaac ggtatttgga aagtaaattt
    51181 gaattggcat tttcccccaa ccagcttctt gtgactggtg gcggtcagca agcaatcttt
    51241 ttgattttac aaacgattct ttcagctgga gaggggatag ctgtagagtc accttctttt
    51301 ttttaccgat tacctctttt caaagcttca ggaacgcgtt tatttgggat accgatggat
    51361 gaagaaggga tcgacttaga aaaactgaaa gaatcgatcc ataaaaataa attgaaagca
    51421 gtgcttatca acccgaattt tcaaaaccct acaggaaaag tgatgagtca aaaacgacga
    51481 gaaaagctag tagaattgtg tcgacagtat cggcttccaa ttattgaaga cgatgtattt
    51541 acggatcttt catttctaaa ccaagaagaa gtccttccta tacgaacgat tgatccggat
    51601 aatgtgttat acgtcggttc gttatctagg gttttaggaa aaacaacgaa aatcggttgg
    51661 gtgataggac ctgagacttt tatctcgcaa ctagctcaag cgcaagaaat gatggagttt
    51721 tcgatgaata tccttacaca aatcacagtg gctaatgtat ttaatgaagc aattgatttg
    51781 aaaatgcaag atgtaagaaa aaagttgaaa gaaaaaagcc gactgataag cgcttgggga
    51841 gattcacaat cgtttttcaa gcttcattca attttaggtg gttactatgc ctggattact
    51901 tgggatggga aaaaactaga ccccgaacta gcagaaaaag tagtagatat gggtcttgga
    51961 attgcaccaa gtctactttt tgggcaagat ataaatggaa tcagaatcaa ttacagtagg
    52021 ctggatgaaa accaaatacc gttgttttat tcaaagatga aaattctaga aaagtggtta
    52081 acagaataat ttcacaaagt aaatagatta aagaatataa cagcttggat ttaaatttca
    52141 agctgtttct tttttttcgg aaaaagaata cgatataaag aaactaagaa aaaaccatta
    52201 tttgtacgtc cgttcacgat gtgtaaataa aataaatgtt tgcaacaaaa ctgtataaca
    52261 ccaacgtttt taaatagaaa cagatggttg taagtttgat tagaaaatag aaaaaatgca
    52321 gatgaaaaat aaaacgcttt ccttattgtg attttattca ctaatgctgt taatatgcgg
    52381 attggcataa tttcctttca tcgtgaaaat ttgtgctttt agcttgtatt tctcataaat
    52441 ccatgataat ttattaatga agacggttaa atagtgatac agttttattc actgtttcaa
    52501 tcgcagaatg aagagatttt cttatagatg agagaggaat gtgtgacaaa tggcaaacaa
    52561 aaaagcaccg atcgacttcc aagcactgct agatgaagtc aatgcagatt tcccagtata
    52621 tcaagcactt gatcaagatg gaaacgtagt taacaaagat ttagtacctg atttatccga
    52681 tgatgaactc gttgaattaa tgactcatat ggtatggtct cgtgtattag accaacgttc
    52741 aacagcgctg aaccgccaag gacgcctagg cttttttgca ccaactgctg gacaagaggc
    52801 aagtcagctg gctagtgctt acgcatttga taaagaagat gttcttcttc caggttatcg
    52861 ggatgtacct caactagtaa aacatggttt acctttatct caagcttttt tatggtctcg
    52921 aggacatgct gcaggtaatt cttatccgga agaattgaaa gctctaccac cacaaattat
    52981 catcggtgca caatatgtac aagcagccgg tgttgcatta ggtttgaaga aacgaaacaa
    53041 gaaaaatgtc gtcttcactt atactggtga tggcggttca tcacaaggtg atttttacga
    53101 agcaatcaat ttcgctggcg cttaccatgc aaatgccgta ttttatatcc aaaacaacgg
    53161 gtatgcgatc tctacacctc gtgaaaaaca aactgcagca aaaacacttg cacaaaaagc
    53221 tgtcgcagct gggattcctg gtattcaagt cgatggaatg gatccgcttg cagtatatac
    53281 tgtatctaaa atggcacggg aatgggcgat cagtggaaat ggaccagtcc taatcgaaac
    53341 actgacttat cgttatggac cacatacact atctggtgac gatccaaccc gctaccgtac
    53401 aaaagacaca gatgacgatt ggcagaaaaa agatccattg atccgcttcc gtaaatattt
    53461 gacggaaaaa ggactttggt ctgaagaaaa agaagaacaa gtcatcgaag caactaaaga
    53521 agaaatcaaa gcagctattg ctgaagccga caaagtacca aaacaaaagg tttcagattt
    53581 cttgaaaaac atgttcgaag tatctccaca atcaatcaaa gaacaaattg caatttatga
    53641 agcgaaggag tcgaaataat catggcacaa aaaacaatga tccaagcaat cacagatgcc
    53701 ttagcacttg aactagaaaa tgacgagaat gtcgttgttt ttggtgaaga tgttgggaaa
    53761 aacggaggag ttttccgggc aactgaagga ttgcaggaaa agttcggaga agaccgagta
    53821 ttcgatactc ctttagctga atctggaatc gcaggattga gtttcggtct tgcgttagaa
    53881 ggatttcgtc cagttcctga aatccaattc tttggtttta ttttcgaagc aatggacgaa
    53941 gtagtagctc aaatggctcg tacaagatat cgaatgagcg gcactagaaa tctacccatc
    54001 acaattcgtt cgccatttgg tggcggtgtt catacaccag aattgcattc ggataattta
    54061 gaaggtttga tcgctcagtc tcctggtatt cgagtagtga ttccttccaa cccttatgat
    54121 gcaaaaggac tattgatttc ttctattaga agtaacgatc cggttgtttt cctagaacac
    54181 atgaaactat atcgctcatt ccgagaagaa gtgccggatg aagcttacga agttcctttg
    54241 gataaagcag cagttactcg tgagggaaca gatgtatcta ttatcacata cggagcaatg
    54301 gtgcgtgaag caatcaaagc agcagacaat cttgcaaaag aaaatatatc tgttgaaatc
    54361 attgatcttc gtacagttgc gccattagat gtagaaacga tcattcaatc cgtggaaaaa
    54421 actggtcgcg tagttgttgt ccaagaagca caacgacaag cgggcgttgc cgcgcaagta
    54481 gtttctgaga tctcagaacg cgctatcctt tcattagaag ccccaatcgg acgagtttct
    54541 gcacctgata cagtcttccc atttggacaa gcagaaaatg tctggttgcc aaatgcaaaa
    54601 gatatcgaag acaaagtaaa agaaatcgcg gaattttaag aaaagaaggg acgaaacaaa
    54661 gtcatggctt atcaatttaa attgccggat atcggtgaag gaatcgcaga aggtgaaatc
    54721 gtcaaatggt tcgtcaaacc aggagatacg atcaatgaag atgatacatt actagaagtt
    54781 caaaatgaca aatcagtaga agaaatcccg tcacctgtta caggaacggt taaaaatgtg
    54841 attgttccag aaggtaccgt agcaaatgtt ggggatgtat tagtagaaat cgatgcacca
    54901 ggacacgaag acaatgaagg ggacagtggt gtagccgcag aatcccaaac tccagctaaa
    54961 ccggctgctg aacccactgt tgatacagaa tcagctggat cgtcaagcga aggagtcttc
    55021 caatttaagt taccggatat cggtgaagga atcgcagaag gtgaaatcgt caaatggttc
    55081 gtcaaaccag gagatacgat caatgaagat gatacattac tagaagttca aaatgacaaa
    55141 tcagtagaag aaattccgtc acctgtcaca gggacagtta aaaacgtgat tgttccagaa
    55201 ggcactgtag caaatgttgg ggatgtatta gtagaaatcg atgctcctgg acacaatagt
    55261 gcaccgtcaa ctagtgcgcc atcagctgaa gcaccaaaag aaaaagtgga aacatcaggt
    55321 tcagcaagtg tcgttgaagc tgccgatcca aataaacgtg tattggctat gccatctgtt
    55381 cgtcaatttg cccgtgaaaa agatgtagat atctcacaag taactgcaac tggaaaaggt
    55441 ggcagagtaa caaaagagga catcgaaaac ttcttggcag gtggtccaag cagtgcacca
    55501 gcgaaatcag aagcgccaga agcggcagca ccgaaagaag cagctcctgc agcagaaagc
    55561 aaaccagctg caccagcaaa accattcaag tccaacttag gagacttgga agaacgtgta
    55621 gcaatgacgc caacacgtaa agctatcgct aaagcaatgg tcaacagtaa gcatacagct
    55681 ccacacgtga cattgcatga tgaagtagaa gtgtctaaac tatgggataa tcggaaacga
    55741 tttaaagaag ttgctgctgc aaatggtacg aaacttactt tcttgccata tgtcgtgaaa
    55801 gctttaacag cgactgttaa gaaataccca gtattgaatg catctatcga cgatgcaaac
    55861 caagaaatcg tttataaaca ttactacaat atcggtattg caactgatac ggatcacgga
    55921 ctatatgtac caaatgtgaa agatgcagac cgcaaaggaa tgtttgctat tgcagacgaa
    55981 atcaatgaga aagcaaaatt agctcatgac ggcaaactat ctgcagaaga tatgcgcaat
    56041 ggaacaatta cgatcagcaa tatcggatct gtaggtggcg gttggtttac accagtcatc
    56101 aactatccag aagtagctat tttaggtgta ggaacgattg cacaacaacc aatcgtaaat
    56161 gctgaaggcg aaatcgttgt cggtcgtgtg atgaaactgt cattaagttt cgatcaccgt
    56221 atcgtagatg gagcaactgc gcaacaagca atgaataata tcaaacgtct tttagctgat
    56281 ccagagctat taatgatgga aggatgataa acaaatggta gttggagatt ttgctgtaga
    56341 attagataca gttgtaattg gtgcgggacc tggtggctat gtagctgcca tccgcgctgc
    56401 tgaaatggga caaaaagtag caatcattga aagagagtat attggcggtg tttgtttgaa
    56461 tgtcggctgt attccttcaa aagcactgat agctgcagga caccattatc aagagtcact
    56521 agattctgaa atgtttggag taactagtga aaacgtcaaa ctagattttg caaaaacaca
    56581 agaatggaaa gaaaacaaag ttgtccatac gttgacttct ggtgttggtt tcctattaaa
    56641 aaaacataag gttgaaacaa tcgaaggaga agcgttcttc gtagatgatc acacactacg
    56701 cgtcattcat ccagattcag ctcaaactta ttcgttcaat aacgcaatca tcgcaacagg
    56761 ttcccgtcca atcgagattc caggatttaa attcggagga cgtgtgttag attctactgg
    56821 cggattagca ttgaaagaag tacctaagaa atttgtgatc atcggtggcg gtgtgattgg
    56881 tgccgaattg ggcggagcgt acgcaaactt aggagcagaa gtaacgattc ttgaaggctc
    56941 accacaaatt ttaccaactt acgaaaaaga tctagtaaaa ttagtagaag atgatttcaa
    57001 gaaaaaaggc gtaacagtcg taacgaatgc aatggcgaaa gaagcagtcg ataacggtga
    57061 cagcgtgaca gtgaaatatg cagtggacgg gaaagaagaa tccgttactg ctgattatgt
    57121 gatggtcact gtaggtcgtc gtccgaatac agatgatatg ggcttagaac aagcgggtgt
    57181 cgaagtagga gaacgaggat tgatcacggt ggataaacaa ggacgtacga acgtacctaa
    57241 catctatgcc atcggtgata tcgttccagg ggcggcatta gcacacaaag ccagttatga
    57301 agcaaaaatc gctgctgaag caatctctgg caaaaaagta gctgtagatt ataaagcaat
    57361 gccggcagta gccttcactg atcctgaatt ggcttctgta ggtatgacga tcaaagaagc
    57421 aaaagatgct ggtatcgaag caaaagcata taaattccca ttttcaggga acggtcgggc
    57481 attatcgctt ggtaaaacag aaggattcat ccgtctagtt acgacgatcg aagataacgt
    57541 gcttattggc gcgcaaatcg gtggagtcgg cgcaagtgat atggtctctg aattagcttt
    57601 agcaatcgaa tcaggaatga atgctgaaga tatcgccttg acgattcatc ctcatccatc
    57661 tcttggcgaa atcgtcatgg atgcttcgga attagcatta ggtttaccaa tccatatttg
    57721 atttgaataa tacaggcgaa acgcaactga aaaatcagtt gcgttttgtt ttttagaatt
    57781 atcaataata ttattatgta aacttaaaaa tatgacaact tcttggttta ttaccacttc
    57841 ctatgctaca ctgaagagga atatgaaata ggagagttga agatgcaaga atatcagtat
    57901 ccacttgatt tagattggac aacagacgaa atggtcattg taatgaagat gtgggaagat
    57961 ctggaacgtg cgaatgaaaa agggataaac aatgaagctt ttttgaagac ttatcagcag
    58021 tttaaaactg ttgtcaaatc cattggtgag gaaaaacgat tgggacggga atttgaaaaa
    58081 gcttccggtt attctttata tcgtacagtg cagcaagcaa aaaaaaatcc aaacaaaact
    58141 ttgagaatga acgggtgaga taaatggaac agatgattga tcatatcaag tcctggcttg
    58201 aagaagctgg tgagaaaatc aaatcttctc aacaaacatt aacaatctcg caaaaatcaa
    58261 atcgaacgga tctagtaaca aatgtcgatc gaaacatcca gaattttcta atcgaaaaaa
    58321 tccattcctt tgatccagag gcgaaaatat tggcagaaga agaagggtgt aatcaattat
    58381 cggatctcga tggaagagtc tttatcatcg atcctattga tggcaccttg aattttgtga
    58441 ttgaagggga aaatttttgt attatgctcg ctgtttatga atccggcgtt ggaaagcttg
    58501 gtttcattta tgacgtgatg agaaatgaac ttttttgggg tggaaaagga ataggcgtgt
    58561 ataaaaatga tgagccattg cctagaccag caaataaatc cttatcggaa ggcttacttg
    58621 gtgtgaatag ttatcttttc ggtcataata aatatggaat ccgagaaatt gcggaaagca
    58681 gcatggggac acgtatgtac ggatgcgctg gtcttgaact agtggcaatc ttgaaaggaa
    58741 accacattgc ttatatttca aaccttagcc cttgggatta tgcagcggga aatgtcttat
    58801 tggaagagtt tggtatggta acaagcggtc tttttggtac tcctttgaca tttgaaaaaa
    58861 gggaatacta tttagcaggg acttcttgtg catatgcaga aatcgcagac cttttgaaaa
    58921 aagaaggctg aaaaaaaggt agaaatcatt cacaaaaatg ataaaaacga gcaaaagatt
    58981 aattttctcg aaaaaatgaa aacattttac tatatttcat gaaaaaagtc tgctataatt
    59041 agcagtcgag taaaatttgc cctgtcataa agaaccaata aaaaaggagt tagttacatt
    59101 gaattataga aacgatatcc gtaacgtcgc aattattgcc cacgttgacc acggaaaaac
    59161 aacattagtt gatgaactat taaaacaatc agatacatta gacgcacata cgcaattgca
    59221 agaacgtgcg atggactcaa atgcgctaga aaaagaacgt ggtatcacga ttttagccaa
    59281 aaacacagct gtagactata aaggtattcg cgtaaacatc atggatactc caggacacgc
    59341 ggacttcggt ggcgaagtag aacggatcat gaaaatggtt gacggcgttg tgttggtcgt
    59401 agatgcgtat gaaggaacaa tgcctcaaac acgttttgta ttgaaaaaag cactggaaca
    59461 acatatcaca ccaatcgttg tcgtaaacaa gattgacaaa ccttctgctc gtcctgagca
    59521 tgtcgtagat gaagttcttg aactattcat cgaattagga gcagatgatg atcagttaga
    59581 ctttcctgtg atttatgcat ctgcactaaa tggtacgtca agcttgtcag atgatccagc
    59641 agatcaagaa ccaaccatgg cacctatttt tgatacaatt attgaaaaaa tccctgctcc
    59701 tgtcgataac agcgacgaac cattacaatt ccaagtttca ttacttgact acaacgatta
    59761 tgtaggtcgt atcggaatcg gacgtgtatt tagaggaaca atcaaagtag gagaccaagt
    59821 agcattaatt aaattagacg gaacagttaa gaaattccgt gtaacgaaat tatttggttt
    59881 ctttggacta aaacgtctag agattcaaga agcaaaagct ggagacttga ttgctgtctc
    59941 aggtatggaa gatatctttg tcggtgaaac agttactcct gtggatcatc aagatgcgtt
    60001 acctatcctt cacattgacg aaccaacatt gcaaatgact ttcttagtta ataattcacc
    60061 atttgccggt cgagaaggta aattcgtaac cgctcgtaag attgaagaac gtttgatggc
    60121 agaacttcaa acagatgtat ccttacgagt tgaaccaaca aactctccag atgcatggac
    60181 agtttctggt cgtggagaat tgcatttgtc tatcttgatt gaaaacatgc gccgtgaggg
    60241 atacgaattg caagtatctc gtccagaagt tattgaaaaa gaaatcgacg gtgtaaaatg
    60301 cgaaccgttt gaacgcgtgc aaatcgatac accagaagaa tatatgggta gtgttatcga
    60361 gtccttgagc ttacgtaaag gtgaaatgca agatatggtc catacaggaa acggacaaat
    60421 ccgtttgaca ttcttaacac ctgcccgcgg tttgattggt tattcaactg aattcctttc
    60481 aatgacacgt ggttacggaa tcatgaacca tacttttgac caatatctgc caatgcttcc
    60541 tggtcaaatt ggcggacgtc accaaggggc gcttgtttcg atcgatacag gaaaagcaac
    60601 gacttattct atcatgagta tcgaagaacg cggtacagtc ttcgtagaac caggtacaga
    60661 agtatatgaa ggaatgatta tcggggaaaa cagccgtgat aatgatttga ctgtcaacat
    60721 cacgaaagca aaacaaatga caaacgtacg gtctgctact aaagaccaaa catctgtcat
    60781 caaaaaacca aaacaattga cgttggaaga atctttggaa ttcttgaatg atgatgaata
    60841 ttgcgaagta acaccagaaa gcattcgctt gagaaaacaa attttagaga aaaatgctcg
    60901 tgaaaaagca tctaaaaaga aaaaataatc tgtaaaagga tgaagagaga aaccagcttg
    60961 ttataaaaaa tagctggttt ctttttttgt ccaactcttt tctgtggaga agtcatcttt
    61021 gtcaatggac ataaattcag aaaatacgcc gaaagacgtt taaaagactt ttaaaaaact
    61081 tatttctaga gagtttttgt ttcataataa gataatgttt cacgaaagga gatcaacttg
    61141 tccttcgaag aaaatcgaag taaaatggca agagaaagga gcgggaagat gaacgactta
    61201 ttaaaacaat actttggtta tgatgagttt agacctggtc aaaaagaaat tatccagaaa
    61261 gtcattgatc aggaaaatgt attaggaatc atgcctaccg gaagtgggaa atcaatctgt
    61321 taccagcttc cagctttact attagatggg cttactgtgg ttgtgtcccc tttaatctct
    61381 ttgatgaagg atcaggtgga cgcggcgaat caattaggta tacccgccac ttttatcaat
    61441 agctcgttag atggttacga aacagctaga cgcttccaag aaatcgatag acagcagtac
    61501 cgtttacttt atatcgctcc tgaacgtttt atcatgccgg attttatcca agcaatgaag
    61561 cgatggaacg tccgtatgat cgctattgac gaggcacatt gtatctctca gtgggggcat
    61621 gatttccgcc caagttattt acagatggcg aaccagctag atcaactgcc aaatcgtcct
    61681 gtaattgttg ctttgacagc tacagcgact gtgcaagtag ctgctgacat aaaaagactt
    61741 ttaaaaattc cagaaaacaa ccatattcag actggttttg aacgagaaaa tctacgattt
    61801 caagtaatta aagatcaaaa aaaagaacaa tatctaatcg aatatttaaa gatcaacaaa
    61861 aatcaatctg gtatcattta tgcagcaacg agaaaagaag tagaccgtct ctatcatttg
    61921 cttaaaaaat tcgatttttc cgtgggtaga tatcatggtg gtctaaatga gaatgagcga
    61981 acagaaatgc aagaagcctt tttatacgat cgcttgcagc tgattgtagc gacaaatgca
    62041 tttggtatgg gtataaataa aagcaatgtc cgttttgtta tccattatca aattcctggc
    62101 tctctagaag catactatca ggaagctggt cgggcgggaa gagatggtct ttccagtgaa
    62161 gccatcctgc ttttctctcc tcaagatatc caagtccaaa agttttttat ccagcagtcc
    62221 caaagagaag aagggcaaaa gcaaaaagaa tatgaaaaat taaaagcgat gacagaatac
    62281 gtgtatattg agtcctgtct tcagcaatac atcctaaact attttggcga gacaagcagt
    62341 ccgtgcaacc gttgtggtaa ctgtttagat gacagagaaa tagtagaagt aacgacagaa
    62401 gcacagatgg ttctttcttg cttaaaacgt atgggtgaga actatggtaa acaaatgctg
    62461 atgaaagtct tagcaggatc gaaagaacaa aagctgcaag ctttaggttt tggacattta
    62521 tctacctatg gactgatgaa aaaccaatcg caaaaagaaa cgatgcagtt aattgaatat
    62581 ttgatatcga atggctatct tctgaccatc aatggtgagt atcctgtttt gaaagtaaca
    62641 gaaagaggga tacaagtatt gaaaggacaa gaatctgttt atcgaaagga acccaagaaa
    62701 gtccagcagc tatcggatga agaaaccgat acactatttg aagtgctgcg ggaactgcga
    62761 acggatctag catcagaagc aggcgttccc ccgtatgtcg tattttcaga ttccacattg
    62821 aaagaaatga gccgaatacg tccatcaagc agattggaga tgcttcagat aaaaggagtt
    62881 ggccaaagca agcttgataa atatggcgaa gcctttttat ctcggataaa aaatgcggac
    62941 tctaacgttc tggataagta gaaagaacga atacgtaaaa atgaataaaa acaactggtt
    63001 aaatctgttt tttttgctcc ttatgaaaat caaatatgtt ataattaagg aattagaatg
    63061 ggaggtaagc acagatggat tctttgtcga aaaaacttgc gatccaagta ttaaatgatg
    63121 aagcagaacg gatcaaacga ttaatccgaa atcaaaacaa cagtctctgt atctcacaat
    63181 gcaaagcctt cgaagaagtg gtagatacac agatgtacgg cttttcccgg caagtcggtt
    63241 ttgctgtccg tataggagta atcggaaaga acgagggcca gctgatgtta agtgagctag
    63301 aacgagaatt gaatcagtta tatagtgagg tttaccaaga aatttatgat aaaaaagaag
    63361 ttggcaagga ggaatagact tgcctaagaa agtgaaaaaa aggcatctat tagactatag
    63421 catcttgatt ccctatttaa ttttatgtgt attgggattg atcatggtat atagttctac
    63481 gtcgtatcta ttattagaaa acggacaaaa tccgtccgca tcagtcatta accagagtat
    63541 tttttgggtg cttagcttga ttgctatcgc actactttat aaaatgaaaa cagatgtttt
    63601 gaaaaaccag cgtttgatca tggcagccat cgcagttttg acgattttgc tgctgattgt
    63661 tgtgtttttt ggtaaggaga tcaatggggc gaaaggttgg ctgcaaattg caggattctc
    63721 gatccaacct gccgaatacc taaaaatcat ttctatctgg tacctgtctc tgactttatc
    63781 taaaagacaa aacagtgtac aaaaagattt tcttgggact gtgaaacgtc cacttgcaat
    63841 ggttatcgga cttactgcat tagtcgctat tcttcccgat tttggtaatg cggcagttat
    63901 ctttttgatt attttggttc tgcttttagc cagcggagtc aattatgtgt atactttgat
    63961 tgtaggagta ggaggctttt gtctaagtac ttttacgatc tggctgatca atatcacaaa
    64021 cggaaagatc ttacccggac gtttacagta tatctataat cgatttgcta tttaccaaaa
    64081 tcctttctca gacgaattaa ataaagggca tcaactagtc aacggctact atgccatgtt
    64141 caacggagga ctatttggca ggggattagg taacagtatc cagaaaaaag gatttttaca
    64201 agaagcacaa accgatttta tttatgcaat cgtagtggaa gaactgggag tgattatggg
    64261 gattttgata ttagcgttgc tattctttat gattgtgcgc attgtccttg taggtatccg
    64321 ttcaaaagat ccgttcaatt cgcttttgtg cattgggatc ggcgcaatgt ttttgatcca
    64381 agtatttgtc aatcttggag gaattactgg cgttattcct ttgacaggga tcacgttccc
    64441 atttttaagt caaggaggct ccagtttgct gatgctttcg atttgtgtcg gctttgtcct
    64501 caacatcagt gcggatgaaa aacgcaagag cttaggttta taataaaaaa agatgtagga
    64561 gtggttcgat gaaaaaagtt ttagttgcta acaggggtga aatcgctgta cgtgtatttc
    64621 gtgcttgtac agaattagga attaaaactg ttggaatcta tgcagaagaa gatgaatatt
    64681 ccgtacaccg tttcaaagcg gatgaagcat atttagtagg acaaggaaaa aaaccgattg
    64741 atgcgtatct agatattgaa gggatcattt ctatcgcgaa agaatgcgga gctgacgcta
    64801 ttcatccggg ttacggtctt ttgtcggaaa acttgaattt tgcgcaacgg tgcgaagaag
    64861 aaggcatcat ttttgttgga cccaaactgc atcatttaga tatttttgga gataaaatca
    64921 aagcaaaagc tgcagctatc gaagcgggga ttgcttcgat tccaggcaca gatggaccaa
    64981 tcgcttctat agacgatgcc ttggagtttg cgaaacaata cggttatccg attatgatca
    65041 aggcagctct aggcggcggc ggacgcggca tgcgtgtagc tcacgatgaa aagagtgcaa
    65101 gagagggcta cgaacgtgcg aaaagcgaag caaaagcagc ctttggtagt gatgaagtgt
    65161 atgttgaaaa atacatcgct aatcctaagc atatcgaagt acagattctc ggggatacgc
    65221 atggaaacgt catccatctt tttgaaagag attgttctgt tcagcgccgt caccaaaaag
    65281 tagtagaagt agcgccttgt gtctctatga atgaacagca acgtcaaaaa atctgccagg
    65341 cagctgtcca attgatgaaa tatgtcgggt atgtcaacgc aggtacagta gaatttcttg
    65401 ttgaaggaga tgatttttac tttatcgaag tcaaccctcg cgtccaagtg gaacatacga
    65461 tcacagaaat gattacagat attgatatcg taacaacgca actgttgatt gctcaaggtt
    65521 tggatctgca taaagaaatc ggattgcctc agcaagaagg aattaaatta aatggttcag
    65581 ccattcaatg ccgaatcact acagaagacc cgttgaataa ctttttacca gacacaggga
    65641 aaatcgatac gtatcgttca cctggtggtt ttggcgtacg tctggatgtc ggaaatgctt
    65701 acgcgggcta tgtagtgaca ccgtattttg attcattgct agtaaaagta tgtacgcatg
    65761 gtgctacctt tgaaacagcc attcagaaaa tggaacgttg cttgaaagaa tttagaatcc
    65821 gtggagtgaa aacaaacatt ccatttatgc tcaatgtcat cacacatcct gaattccaat
    65881 caggaaatgc aaaaacaacc ttcattgata gtacagcaac attatttgaa tttcctcgac
    65941 tacgagaccg cggaaacaaa acgatgaaat atatcggtga aattactgtg aatggttttc
    66001 caggtatcga aagtggcgaa aaaccatttt atgaagaacc acgtatgcct aaagatctaa
    66061 tcacaagaac tgattacgta acggctaaga atgtattaga tgcgaagggt gccgatgctt
    66121 tggtcgaatg gatcaaagga caggaaaatt tgttgttgac ggatacgaca tttagagacg
    66181 cccatcaaag tctgctagca actcgtgttc ggacaaagga tttcaagcag attgcacgtt
    66241 tgactggaga aggtcttcct gaactctttt caagtgagat gtggggggga gcaacatttg
    66301 atgtggctta ccgctttttg aatgaagatc cttggcaacg gttgagaaaa attcgttctt
    66361 taatgcctaa tacgttgctg caaatgcttt tcagaggttc aaatgccgtt gggtattcta
    66421 attatcctga caatgtattg gtggaatttg tcaaagaagc agcagcgcaa ggaatcgatg
    66481 tatttcgaat ctttgatagt ttgaactgga ctccacaaat ggaaaaaagt atccaagctg
    66541 tacgggatac tggtaaaatc gcagaggcag ctatttgcta tactggcgat atcaacgatc
    66601 caagtcgggc aaaatacaat gttcagtatt ataaagacat ggcaaaggaa ctagaacaac
    66661 tgggtgcgca tatcattgca atcaaagata tggctgggct gcttaaaccg caggcagctt
    66721 atcgtttgat tagtgaattg aaagaaacga cagatctgcc aatccatctg catactcatg
    66781 ataccagcgg gaatggaatc atcacttatt ctgctgcttc aaaagcagga gtagacatcg
    66841 ttgacgtggc aatgagtgca atgagtggga atactagtca gcctagcatg agcagcttgt
    66901 attatgcact ggtcaatggt ccacggctgc cagaaatcac gattgaaaat gcacagaaat
    66961 taaaccatta ttgggaagat gtgcgcatgt attacaaacc gtttgaaaac ggcttgaatg
    67021 ctcctgaaac agaagtatat atgcatgaaa tgccaggagg acagtattcg aatctacagc
    67081 aacaggcaaa agctgtcgga ttaggtcatc gctgggatga aatcaaacaa atgtatcata
    67141 ctgtcaactt gatgtttggc gatatcgtta aagtaactcc atcatctaaa gtagttggag
    67201 atatggcact atttatggtc caaaatgatc ttacagaaga agatatttat gaaaaaggcg
    67261 aaacactaag tttccctgaa tctgttgtga ctttcttcca aggagaacta gggcaacccg
    67321 ttggcggatt tcctaaaaaa ttgcaaaaga ttattcttaa aggcagacct gcattgaatg
    67381 aacgtccagg tcttttggct gaatcagtcg acttcaacga agtaaaaaaa gaattggcag
    67441 aaaaaattgg ctatgaacca aaacaagacg aagtattaag ctatttgatg tatccgcaag
    67501 tctttttgga ctatcaaaaa gcatataatc aatttggcga tgttacatta ctggacacac
    67561 caacattttt ccaaggtata cgtttgggtg aaacaatcaa tgtacaaatc gaacgcggaa
    67621 aaacgcttat cattcgttta gatgaaatcg gagaacccga tatcgaaggg aatcgcgtac
    67681 tgttcttcaa tttgaatgga caaagacgtg aaatcactat caaagataat tcgatcatca
    67741 gtacggtcca aacccgattg aaagctgaac cgacaaaccg agaacaaatt ggagcaacga
    67801 tgtctggttc tgttttggat gtattagtga aaaaaggcga taacgttaaa aaaggtgata
    67861 cgttgatgat tactgaagcg atgaaaatgg aaacggccat cgaagcacgt tttgacggag
    67921 aagtcgcaca tgtatatgtg tcttcaggag acactatttc gtctggtgac cttttgattg
    67981 aagtgactga gaaataataa gcgaaaggtg gggaaacatg aaaagactgg gtttgttttg
    68041 gctgattctg ctattagtta ttgtggcatt ctacatggaa ccagtcttgt ttccgccaaa
    68101 acttgaagtg aaggaaaact ctgatcaaca aatatcaaac caaatgacaa cgtggaaata
    68161 ccaggagctt aaagcaactg gtttttctac ttatatcgga aaaccgatcg atcagctgga
    68221 aaaggatttt ggccaagcct atgatcgatt aactagcgga tttggttttg aaacaagata
    68281 ttatgaaaat aaagctagtc atctcacatt tgaagcgaac atccaagaca gcagagtcac
    68341 aactgttaaa gtactgcaaa cagggcctgc tgatatcgcg ccgttttatt tagggatgac
    68401 gatgcaagat cttacgaaaa tcactactat ctatactaat ttcacttttg agtaccatga
    68461 tacagaagtt ggaatcgaac taatggaaga ggatatgaat tatcgtccgc taattgcttt
    68521 tgataacgac acttttgcta ttttattttt tgatcaaaca acaaacggcc tattttcagt
    68581 tgtgtattta gataaagata gtcttttgaa actattgcca tatcaagtgt ttggtgaagg
    68641 ccttccacat tatcaagcag ataaatcggc tgattgggaa tcaatcaatc gcgttaaaga
    68701 acgaaaaagt acgactttat taaatatgct tagaagagaa gacgaacttc ccatatacaa
    68761 tcaaacactt gcctttcaaa accacagtca gctgctacta catgattttt tgacaaggcc
    68821 tgaggaaata ttgtcagagg aacgtatggc tgattggcaa aaatcgctag gcagtgctca
    68881 aacgacagtg aaattctcac tgacgaatga tgaattggag aagttaatca gtaaacaaaa
    68941 attagaaaat actagtgggc tatttattca tccggcgatt gacgctactt tttcgttttt
    69001 atattggtac agcgatcctt acaaccatga ccgtttcatg gacccgggaa cggattctgt
    69061 aggcattgct ttttctaaag aaaatatgtt agtcttattg caagaagaaa acggagaaag
    69121 tagtgatagc agtgatcgtt aacgaagagc tgtttcaact ggaggatcaa tgcctccaag
    69181 tcgctgaaat gataaaaaag agtaaaacga tggcttctta tcttgaacat aaacagtcaa
    69241 tggaagaatc tgcaaaagtt tgccagttga aagaagcgtt cttgtctaag aaagaagcat
    69301 ttgaaaatgt agcggcatat ggtgagtatg cacctgatta tcgtgaaaaa aaacgagctg
    69361 ttcgaaaagc aaaaagacag cttgatatgg atgagcatgt tgcagctttc cgatatagtg
    69421 aaacacagct gcaaagtctg ttggatcaaa ttgtccaagt acttgccgaa gaagtttcta
    69481 atgaaatcaa agtagatgca ggtagcccgt tttttgaaac gaaaaaacac tttggatgcg
    69541 gaggtaattg tcatgcaatt agacaatagt gaatttgaaa taacaaaacg acgcgggctg
    69601 atcgtatggg tctacagctt aaaacaatta aagaatctga aaaagttcgg cttgttgcat
    69661 tatgtctcaa gaagaatgaa atatgtcgtg ctttacttaa atgaagagac attggaagaa
    69721 acagaagaac gaatccgtaa attacatttt gttcgaaacg tagaacgttc ttaccgaccg
    69781 gatatcgaaa tgaattttgc cgaaaaaata ggaaacaaaa cagtattaaa agacgaaggt
    69841 ttcgaaattg aagagctaaa tacagaaatc cgattagctg aacatcttta aaaatctctt
    69901 atgagcagtt tttaaataaa aaataacttg gataacgatc gatttttgat cattgtccaa
    69961 gtattttttt ggtatactag taccagtacc atttctaagg agtgagtaga tgcggataat
    70021 atcaggaaat tatggcgggc gccgtttaaa aagtttagca ggggcgaaca ccagaccaac
    70081 tacagataaa gtaaaagaat ccatattcaa tatgattgga ccatattttg atggagagac
    70141 agtattggat ctgtttgcag gaagcggtgg tttagctatc gaagcagtat ctagaggctg
    70201 cagtcatgcg gtatgcgtag acaaaaatta ccaagcgcta aaaatcatca aagaaaacat
    70261 cgaaattacg aaagaaccag agaaatttac cacaataaaa cttgatgcgg acaaagcaat
    70321 cgaacggcta gcagcggaga aacaacaatt cgactacttg tttcttgatc cgccatatgc
    70381 aaaacaaaaa ataatcgatc aaatccataa aatggaagaa ctgggacttt tttctaaaac
    70441 cgcagttatc gtatgcgaaa cggataaaac agtcgaattg ccagaaactg tggctggatt
    70501 ccatcaaata agaaaacaga tttatggcat aacggccgta actatctata gaaaagaggg
    70561 aaatcaatga aacgtgcatt attcccagga agttttgatc cttttacaaa aggtcactta
    70621 gatacggtgg aacgtgcggc taaattattc gatgaggtag taattggtgt ttttatcaat
    70681 acttcaaaga aaagtttgtt tcccccagaa gaaagaatga cattgatcac aaaagctgtt
    70741 tctcatcttc ctaatgtaaa agtcatgcat caagaaaatc aattgacggt agaaacagca
    70801 aaagagattg gtgcagatgc gttgatcaga ggcatacgaa gtatcaaaga ctttgaatat
    70861 gaacgagaga ttgctcagat gaatcatcat ctatatgatg aattggaaac tgtcttctta
    70921 ttagcaaaac cagaatatag tcacgtgagt tccagtattt taaaagaagt actgcacttt
    70981 ggcggagatg tgagcagcta cttaccgcca gtgatcaacg aagctttagc tggaaagcgt
    71041 gaatcaaatg aaagctaaac aatggttgaa aacagtactg attatcatat ttggaatgtt
    71101 gatcattgga tttgtagtag ttcccttgcc gtattatgta gaatcaccgg gagccacgat
    71161 cgatctgaaa gaactaatta cggtcaatga taaggaagat aaaggttctg ggtctttctc
    71221 tcttacctct gttggtatca ggcaagctac aggtttctta gctttaaaag ctaaaatgac
    71281 cccttttgaa gaattgatca gtgaagaaga attgactggt ggggcgacaa atgaagaata
    71341 taatcaaatg caacagtatt atatggattc ttcccaaaat gcagcaatcg aacaagcgtt
    71401 aaaacttgca gacaagcctt ataaaatgga attcaaaggc gtatatgtca tgagtgtaga
    71461 gaaaacatcg aatttctttg gcaagctttc agttggagat acagtaacaa aagtagacgg
    71521 gaaaagcttt caaagtacag aagcatttat ggattatgta aaaagccaaa aagttggaca
    71581 aacaatcgaa atcagttatt tgcataatgg tgaagaaaaa acagcttctg gtgatctgat
    71641 cgagttacca acagataaaa aggcaggaat aggtatcacg cttactgacc ataccgagat
    71701 cgaatcagat acagatgttc gtattaattc tggctctatc ggtgggccat cagcgggatt
    71761 gatgttcact ttagaaatct atgaacaagt cactggtaaa aacttgcgac atggtaagca
    71821 aattgcagga actggaacga tcaacagtga aggagaagtt ggccgaatcg gaggaatcga
    71881 taaaaaagtg gcaagtgcgg ataaagctgg cgtagagatt ttctttgcac cagatgatac
    71941 gatttcagac gatgttaaga aagaatatcc tgaaataaaa accaactacg aagaagcaaa
    72001 agctgccgct aaaaaattga attcctcaat gaaaatcgta ccagtaaaaa cagtacaaga
    72061 agcgcttgac tatctagaga aaatgaaatg aaactaaata attaaagagg ggttgtgaca
    72121 cgagttctgt cacaacccct ctttttcgtc aaaaatcttc acgataaaaa taaaaacaaa
    72181 agaaaaaatt gacgtaatta gatgttgaag ggacgatttt gccagagctc actgtttctt
    72241 caattttaaa tgaaaggagt aatgaatgat ttttactggt cattctttga aaaaagaaca
    72301 atgatagaaa aaatcagcaa gaaaatacta gcttcaccca aattgatcat ttggatgtct
    72361 atgatagttc ttatcttttg tatcgcaagt ggttttttat tatcaaattt gctattttat
    72421 agagaaacag aagagactgc tgcaaaagaa atactttctt cgtttagttc cagtggagaa
    72481 gatccatcag ctgaaggaaa cgatgtagaa gaggatgctc gtccaaaggt catgtacacc
    72541 gatatcaaag gaagtgtgaa ggagcctggt atctattcct tttcttcaga agaacgggtc
    72601 tatgatgtac taaagcgtgc aggtggatta ttagaagaag cggatagcga tcgaatcaac
    72661 ttttctgcga agatagaaga tcagcaagtc ctatatatcc ctgctgtagg tgaagagctg
    72721 ccagaacatc ttgatcagtt ggcatcctcg gaaggaaaac agcctacagc agattccgag
    72781 cctcccaaaa taaatatcaa tacagccact ccatcagaac ttcagcagat tcctgggatc
    72841 ggatcagtca aagcgcaaga aatcattcga tttagagaag aaaacggatc cttccagaaa
    72901 gtggaagacc tgcaggagat ttctggtatc ggtgaaaaga cagttgaaaa actaaaaaac
    72961 ttcgttacaa ttaaatagaa tgattcaaaa tcgactggca cgagcaataa atggacgcta
    73021 atggatacta agaataatcc aattctaaga gcttggcagg acgaggaggt agtatttttg
    73081 gataggaaat agcttgattg ttcgcaaaga aacattaaaa aaaccaatgc tgaatgtcta
    73141 tctccaaaat aaaatatctg ggattcatat tatgaatacg gctgtcagcg gcaataacag
    73201 tcaagctcta cgagaacgct ttgccaaaga tgtacttagc tatacagcag ataaagtatt
    73261 tattctcatt gggacgaatg atctagcaga gcataaacaa ctttctaaag aaacgtacca
    73321 aaaaatctgc agtggatgat agttcagctg aaaaaagact atagttctga tcatgctttc
    73381 tttatcacgc caccagcagt cgatgagaca aaacaaaaat atcggaataa caagttgatc
    73441 aaagaatata tctctgttct cacagaagta gcaaaacgcc atgattgtga agtatcgatt
    73501 tttatacgga atttcttgaa caaatggatt cgaagaattt ttataatggg ctaatggatg
    73561 acggacttca tttcggcagt gaagggtagg atcttttgtc agacaggatg attcgttata
    73621 tttaaagaat attttgttta agcaatgagt actttttaat caacttgaaa aaacggagga
    73681 atataaaaca tgttagaaaa atcttcaaaa gcatgcgata cgaataaaga aaaagttact
    73741 gataagtcac ataatgaacg cattccttgg gaccagtact ttatggctca ggcagtactg
    73801 ttatctttac gtagtacgtg tacacgttta gaagtaggag cgacactcgt tcgagaaaaa
    73861 aggatcattg ctggcggcta taatggagca gtcagtggag atgtccactg catagatgaa
    73921 gggtgttacg tcgtggatgg acactgcttg cgaacgatcc atgcagagat gaatgcactc
    73981 ctacagtgtg caaaacttgg tatcccgacg gaaggttcag aaatctatgt cacacatttt
    74041 ccatgtttgg cctgtacaaa ggctttgttg caagctggtg tgaaagggat caattatctc
    74101 catgactatc gaaatgatcc ttatgcccaa gcgttgatcg aacaattagg tgtatctgta
    74161 caccaagttg ctttagactc acagcatttt tcaaaacttc aaaatgaatt aacaggaaaa
    74221 catgtctagc gttttagaaa ggcatgttcg aaatcggttg atttttccgg caattacgtc
    74281 gatgattatc tgtttgttgt attacactga caattggggt tatcgtttgc ttcttgccgg
    74341 aattttttta tggttttcgc taaaagaagg acgttttatc gcgtacttgt gtattttgtg
    74401 tagcataacg gtatccattt cttataggct acaagagtat tcatttttag aggaaaaaac
    74461 aacatctgcg caaattactg tatttacaga tacgattcgt gcaaatggta attttttgac
    74521 aatggaagga aggttatcag agaataaaca aaagctccag ctttcttatc aagtaaacac
    74581 agaagcagaa ttgaaaacat ggcttaactt ccgaggaaag ggaatagaat tgactgctga
    74641 aggcgagttc atctctccag caaaaaagcg gaatctttat aattttgacc aagaagctta
    74701 cttcaggaac catagaattt ctggacggtt ccaaattcaa aagattgaac agatcaccat
    74761 gcaaaatcac tggcgaaact ggcttcagag aaaaagagga tcttttatct cctttgtcca
    74821 atctgaattt ccgcaaaaaa caagtgtata tattaattct ttattatggg gctataaaga
    74881 tgcaggattc agagaaccag aagaaatttt tagggagagt ggtttgctgc atctgttcag
    74941 tctatcgggt ctacacatcc aattttattt agggtggctg tattatttat ttcgacgtat
    75001 cggatggcca ctacacgtca gtattattcc gttgagtcta ctaactatct gttatgtttc
    75061 tattgcagga agttctattt ctgttttgcg tgctagtttg ctgtttttat tgcgattgct
    75121 agtcaaattg ctgaataagc attattccac catggactta tttgcagttg tcttttggct
    75181 tcttttacta tttgatcctc gatttctttt tcatgcgggt ggacagctta gttttttcat
    75241 gagttttttg tttatcctga tttcttttga tggagattgg ttgaaaaaaa cggcctctca
    75301 cgtcctgttt actatgatca cgatgccgtt gattgtatgg catttttatg agtggcctat
    75361 tttaggtagt ctttttacat tactgattgc accggtattc aagtttcttt ttcttcctgg
    75421 tgtgttattt ctcacactct tcaatcgctg gctgccggaa actttacttt tgctgataga
    75481 agagggatta atcttatttg agcgaataat cgatttttct tcagggtcgt ctctcattat
    75541 aggacgtttg ccccttgtgc tatctgggat gtatattttc ggattactga tttgtatgga
    75601 taggttgaaa gaacaacctg tgaaacactt gttttatgtt ggtctttttt cttttatttt
    75661 tttgactttg ccatttttac gcttttcttc aacgatcgct tttatcgatg tcggtcaagg
    75721 agacagtatc tttttgcaaa gtccttttcg aaaagaaacg attttgatcg atactggagg
    75781 tcagttacac tttgaaagag aaaagtgggc gcaaggtatg atacgtcctc ctgctgaaag
    75841 aaatatcgtt ccatatctaa aaggtcaggg gataagtgta atcgataaac tcatcttgac
    75901 acacgacgat acggatcatg tgggagaatt acccacgtta tctcagcatt ttactatcaa
    75961 aaaaatttat atcggttggg gcgctggacg aaatgagcca ttacaaaggc atttatctga
    76021 agctcaaaaa aatggtactg agatcattga aatcaaacat ggggacagga tcagcggtta
    76081 ttatgatctt tatgtattga ctccttttga aaagggagag ggaagaaatg aagattcgat
    76141 tggtctatgg atggaatata acaataggcg ctttttattt ttaggagatt tgaatcaagc
    76201 aatggaaaaa caattgcttt tgatttatcc taatctgaaa gccgatgtag tcaaattagg
    76261 tcatcacggt agccgtacgt caagtaacac agattttctt tcccatattg aagcaaaaca
    76321 tgggattata tcctgcggcg taaataatcg ttatggtcac ccgcacagcg aagtattgga
    76381 gacactagaa gaaaatcaga taaaaacatg gcgaacggat caacaaggaa tgatcaccta
    76441 tcgctggcat ccagtttttc atcctcatgg gctagtggaa acaatgatag actagtaatt
    76501 ctcatctgct catggtagga taatttttga aagtggtgag taatttgagt atacaagcag
    76561 agttacaaaa aatacgtaaa gaaaaacttg catcttgtta cttgatttta ggaacagaaa
    76621 agtttctcca agatcaagtt cggacagaaa ttgtcaaaaa aatccaatta gagggcgaag
    76681 atgacttaaa ttttttaagt tttgacatgg aaaatgcaag tttagatgag gtagtagctg
    76741 aagcagagac gttgccgttt ttcggagatc aacggttagt attcgtagag aatccttatt
    76801 ttttaacagg tgaaaaagta aacaatggaa tcgaacaaaa tactgattta ttgacggctt
    76861 atttgaaaga accattagaa tcgactgtat tagtattctt tgcgccatat gaaaagttgg
    76921 atgaacggaa aaaagtaacg aaacaattga aaaaaacagc aattatcatc aatgtccaac
    76981 agttgaatga aaaagaagta cgtcaatatc tgatgaatac cttagagaat tcctcaatca
    77041 aaatggaacg aagtgcaatt gatttattct tgaggttgac cgacttagat ttatccaaaa
    77101 tgatgagaga gttacagaaa ttggtccttt atggacaaaa tcaacagcaa atcacggtaa
    77161 gagaagtaga agaattagtt cctaaaacgt tagaacataa tatttttgac atgacccaat
    77221 atattttaaa agggaagacg gaacaagctt tgcgtttata cgaagattta gttatgcaag
    77281 gggaagaaac aatcaaaatt aatgcgattt tgctttctca attacgttta tttttacaga
    77341 caaaatttct tgtaaagatt ggttatcagc aggcaaatat tgctgaaaca ttgaaaatcc
    77401 atccatatcg tgtaaaactt gccatgcagg aagtaaaaaa attcgatgaa catttgctgg
    77461 tacagttgtt tgatcaatta gttgagatgg attatcaaat caaaacggga caaatcgaaa
    77521 aagaattaag ttttcaacta ttcgttttgc gcacagggca gttgttaaga aatcgataag
    77581 aagaaagacg agggcaaagc atgcgggaag gtcgtttgtt tttgtccttt ttttgacaaa
    77641 taaaaaaatc ggccgaagcc gattctttta tttagcgatt tttttgctta aacgagattt
    77701 gtcgcggtta gctttgtttt tgtggattag accttttgat tcagccatat caatagcttt
    77761 aacagcttct ttatataacg catctacgtt gtcagcacct gaagctacag catcttcaaa
    77821 ttttttgatt gcagtacgca tagcgcttgt ttgagatgaa tttttcacat tagcgttttc
    77881 gcttgtacga acacgtttga ttgcagattc aatgtttggc attagtttca cctccgataa
    77941 cttaagttct tacgcgtgtt accgcataat tcaacattca ttattatacc taaaggcagc
    78001 gtgctttgca atatgaacat gacaagtttt tttccttaac agtcttttat taggattttt
    78061 tttcagtaaa aacggatagt acaagctgaa aaaaatcaaa aagacgctat tgtgggaggc
    78121 aatagcgtca aaccaaacta gatttttatt tatctattta aacaaagttt gaatgggaat
    78181 gtttggtact aagttattat ataacaaaga gaaccgtttg caaagttttt tttgaaaagg
    78241 gatacgattc cctgttgttc tttgtttttc tattaagaaa ctgcagttat tttataacca
    78301 gtatcctttg atcagtaaag gctattctgg tgtaccgatt attttaatgt attcatcgat
    78361 attttttatc aacatttttt ttatgaccaa ctggttccga cggttccatt tgccacttac
    78421 ttccagcaca tatttattta ggggcaaacg taagatatat tttgccaaat agcgatcttt
    78481 aagcaggcca ttgatcacac ggtcctcaag ctgcaagttg atcccagtca gcatcattgg
    78541 ctgttcttgt aaaacgtacg tatcaattaa aagaccttca aaagtttcca caagcgtctt
    78601 cctttctttt caaagtaacc tgaaaacagg cactaattct tccattttga tattcgttga
    78661 agtggatgcg atcattcttt cttttgtcca ctgtaatcta gtataacaaa aaagagcaga
    78721 agagccaaaa agaaaaaaga gcatcgaatt ctataggaag tctcattggc acggcctaaa
    78781 ttattacaaa acattgatta aaaaaagccc ctgcatcgca ggagcatccc ctatatcgtt
    78841 ttaataggtc aatcaaaaag cagtatcaaa cacattttgt atcaaatcag gaaataatag
    78901 ataagctaag cccagaatca aaagtaccgc tagaagaaac cagccgattg ttcctatgaa
    78961 atactttaat ttttcgaaca ttgtatcacc tcgttgtcag tatatcacgg atcagtaaat
    79021 tgggggtgtt aagaatctcc taaaatagac aaaatttgtt ctattttcct aaagcaaata
    79081 cttgtaagat acagtaagtg ataccagaag caatgaccgg tccggcggcg attcctctta
    79141 agaagacaac acctaaaatc gtgccgaaaa caagtgcaac agtgatttgt ggatcagaag
    79201 caatcaatcc tacacccttg gaagataaga cagccactag cccaccgcaa aaaacagcta
    79261 cccagccaat aggggattta aaggcttcta atagatcacg tagaccgatt tctcctgttg
    79321 cgatagggat tagaatagcc gctgtgatga tcgtcacccc aatattgatt cctttgcctt
    79381 gaagccaagg gaaaaaggaa cgggaggctg gtatgagctt cataatcaaa acaaaagctg
    79441 ccgctatcat cagactttga tttttagcta aaattgctac aagtaaaata attcctaaaa
    79501 aaagccaact ttccatgaaa tatattcctc actttcttcg aaacaatcat aacatgtttt
    79561 tagaaagtta caaaagtagg aattcatcct tcaagaccta ttcataagct gtttttttca
    79621 taaaataaac aaaatcataa aaaaacactt tgcttatttg acagaagaca gccgcgcttt
    79681 gtatagtgga caagacttac atttttttga agaatcagaa agatcaggta aaggtcttag
    79741 taaacgcaag ccattcagaa taaccaatat tgtgcttcct tcgtgtccta caactccaag
    79801 aggtagattt aaaaattgca ggaagttaga agcaatcaac aagagaataa caagaatcga
    79861 aaaaataata ttttgtttga tgatccgccg catttttaaa gatagccgat gactgtagac
    79921 aaatttattt aagtcgcttt tcattaagac gatgtctgcg atatccatag cggcatctgt
    79981 cccttgcccc atagcgacac ctaaagaagc ggctgctaaa gcaggcgcat cattgactcc
    80041 atcaccaacc atcgcattta cttggtaaag ttcattctct tttttgacta gttgtgtttt
    80101 ttcttccgga gtacagcttg catagtaatg ttcaatccct acttgttcag caatggcctt
    80161 tgctgtgcca gaatggtcgc cggttatcat acttgtatgg atgttttggg atttaaagta
    80221 ggaaattact tgttgggtat ttgcttttgg aatatctagc agacctaaaa cagcaattaa
    80281 ctgctgatct ttagaaagat aaatcacggt tttgccttga ttttccaaac gttcgatcat
    80341 ttccgcaatc tctttgctga ttatcatcgt ctcaggatca tatgcgtgct tgccaacttt
    80401 ccattgcgca cctaaatagg tagtttgtaa tccaaaacca gtaatttctt ctattggtaa
    80461 gtattggatt tcttcaggta ttgtgcaatc gaaatattgc aaaatcgcat gagcaagtgg
    80521 atgcgtcgtt ttttgttcca tagcaacaag aagttgttgt gccaaatcct tttcatcaaa
    80581 gaaaaagctg tcagttacaa ctggttttcc tttagtgagc gttcctgttt tatcaaaggc
    80641 aatcgctttt aattcagaaa gcttttctaa atggataccg cctttgatta aaatcccatt
    80701 ttttgcagaa tttgaaagag cagctagaga agctggtgta gcagatgcga caagcgcaca
    80761 aggagaagcc acaactaata ataccattcc tcgataaaag ctttcatccc aactccaacc
    80821 taggaaaaag tgggggagaa ggatcattag aggaacagca agcaaaatca attttacata
    80881 attattttcg attcgttcga taaatgaagc tgtttgagtt ggcgtattct gcgcttcttc
    80941 tactaattgg ataatcttag caaagacagt ttcggaactc gtttttgtta ctcgaatcgt
    81001 gatgggattt ccttgattca gcgttcctgc atacatctca tcgcctaaat atttttctac
    81061 aggcacagat tcaccgctta atgcagactc atcgatcgtt gcctgtgtgc tttctaaaaa
    81121 gccatctatc ggaacagctg ctcctttagc gacaaatacc aagttatcca cctttagttg
    81181 agaaacatct acttcttgca tttgtccatt ttcttttaat aattgggctt tttgtggttg
    81241 aagattcatc aaagcagtaa tttctttttg acttttatta gttgtgtatt cctctaatgc
    81301 accgcttaaa caaaagatga atgtcaacat tgctccttca aagtaattac cgatcaaaca
    81361 ggcaccaata gcagctaaag ccatcaataa atccacattc agtgtccgat cattccacaa
    81421 ttctttcaag ccttccgatg tttgtttata tccaccgatg ataatggcac tgatataaaa
    81481 aagtggggtg atggtaagag agaatcggtc gaagataaaa ccgaagatca taaataaaag
    81541 gcagccgatt gttgctgcaa aagctttttt ctgattagtc atcgttatta gacctccttg
    81601 tcaaaacaag gatagcataa taaaaatgta attgataata ataacagcta aatgagaacg
    81661 ataatggtta tcatttggtg attaagcttg atgttgtttt tattatttat aatgattatt
    81721 attaaagaaa ataaaaaacc agaacatttc tcaaataaga g
//
LOCUS       DARBET010000005        73403 bp    DNA     linear   BCT 07-NOV-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4027
            SAMEA114569841-rid20112183.denovo.005, whole genome shotgun
            sequence.
ACCESSION   DARBET010000005 DARBET010000000
VERSION     DARBET010000005.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA114569841
            Sequence Read Archive: ERR12203666
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 73403)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 73403)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001972618.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001972618.1
            Genome Coverage       :: 132x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/07/2023 13:18:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,041
            CDSs (total)                      :: 2,975
            Genes (coding)                    :: 2,908
            CDSs (with protein)               :: 2,908
            Genes (RNA)                       :: 66
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 67
            CDSs (without protein)            :: 67
            Pseudo Genes (ambiguous residues) :: 0 of 67
            Pseudo Genes (frameshifted)       :: 26 of 67
            Pseudo Genes (incomplete)         :: 28 of 67
            Pseudo Genes (internal stop)      :: 20 of 67
            Pseudo Genes (multiple problems)  :: 6 of 67
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..73403
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA114569841-rid20112183.denovo.005"
                     /isolate="VRE4027"
                     /db_xref="taxon:1352"
                     /country="Denmark"
     gene            175..489
                     /locus_tag="SCJ86_000404"
     CDS             175..489
                     /locus_tag="SCJ86_000404"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000421280.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdcP family protein"
                     /protein_id="HEG1167992.1"
                     /translation="MELKYVIPNMEKTFGQLEYAGEGNIEQRRINGRPTILSRSYNLY
                     STIQRADDIVVILPSEAGEKHFESDEKVKLINPHITAEGYKIGNRGFTNYIMHADDMV
                     KA"
     gene            510..887
                     /locus_tag="SCJ86_000405"
     CDS             510..887
                     /locus_tag="SCJ86_000405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001234189.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdcP family protein"
                     /protein_id="HEG1167993.1"
                     /translation="MRLAEGIVIEKDQTFGTLKFSALRREVRIQNEDGTVSEEIKERT
                     YDLKSKGQGRMIQVSIPASVPLKEFDYNAEVELINPVADTVATATFRGADVDWYVKAD
                     DLVLKGQTKPHQKYKKEASESEK"
     gene            897..1670
                     /locus_tag="SCJ86_000406"
     CDS             897..1670
                     /locus_tag="SCJ86_000406"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002388713.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEG1167994.1"
                     /translation="MTNTPLILKEIPKDEAISFIRQYHYSKILPRLCKYFLGIFSEEK
                     LLGVVELGWGTQPLQTIRKLFPDSSLQTTDYLEIGKMCFLPEMNQTNYFGSQALSALI
                     KWLKEHTDCHFLYTLADGIEGKCGYVYQASNFFYCGYFKTSVYRDKQSWEKIHPRSAR
                     LLLEENARFEQVEKKHWLSQAFCEYKGIEKINGRMFRYLYPLTKEAKKLLGHTLYRRH
                     YYPKEKNLRFEKRIAYQKYEAISQPTFDKQARIYNTQLF"
     gene            1692..3095
                     /locus_tag="SCJ86_000407"
     CDS             1692..3095
                     /locus_tag="SCJ86_000407"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001130245.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF87 domain-containing protein"
                     /protein_id="HEG1167995.1"
                     /translation="MPLLHDRGERIKANDKHLVYHFVLISLLVVFLVVLLPFHWRALQ
                     SVNWQAVQLDTFFYQLHTPYFWSSILTALFVCGLISWLYYHYRIDSIKQLEHRQKLAR
                     MILENHWYEAEETQSEGFFKDLPSKKTKQRIRHFPKIYYRMKDGLIHIHVEITLGKYQ
                     DQLLHLENKLETGLYCELVEKTLKDSYVEYVLLYDTIGNRIGIEDVVAQDGRVKLMES
                     VYWEFDSLPHMLIAGGTGSGKSYFILTLIESLLHTNAKLYILDPKNADLADLGAVMNN
                     VYYRKEDMLACINQFYEDMIDRSETMKQHPNYKTGENYAYLGLSANFLIFDEYVAMMD
                     MLGRESTTVIHKLKQIVMLGRQAGFFLILACQRPDAKYFSDGIRDNFNFRVALGRMSE
                     MGFGMMFGSDTQKQFFLKPIKGRGYVDTGKSVISEFYTPLVPKRYDFLGEIGKVIQKK
                     QSEPVPHEVKGTGSDLL"
     gene            3277..4461
                     /locus_tag="SCJ86_000408"
     CDS             3277..4461
                     /locus_tag="SCJ86_000408"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000426690.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation factor domain-containing
                     protein"
                     /protein_id="HEG1167996.1"
                     /translation="MENEKIWIKELKEKRLAYGVSQNKLAVASHITRPYLSDIETGKA
                     VPTQQVKEDLLNALERFNPDNPLEMLFDYVRIRFPTNDVAQIIGEVLRLNMDYMLHED
                     FGYYSYPEHYRFGDIVVLVSHDVSKGVLLELKGKGCRQFENFLLAQHRSWYDFFQDCM
                     EHKGIFKRLDLAINDKTGILNIPDLAKKCKQEECISVFRSFKNYRSGELVHRDEKPYM
                     GNTLYIGSLKSEVYFCIYEKDYEQYVKNDVPLEDAEVKNRFEIRLKNDRATQAMKDLL
                     AHQQAEKTAFEIINRYIRFADKDDTKRRSDWKTNERWEWFIGKNRGALRLTTQPEPYS
                     FERTLNWLHHQVAPTLKIASILDVLNGTTIISTMIQEAKLTEKHEKLIEQQHLAMEDL
                     IT"
     gene            4458..4748
                     /locus_tag="SCJ86_000409"
     CDS             4458..4748
                     /locus_tag="SCJ86_000409"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000055367.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEG1167997.1"
                     /translation="MSLLTKELKKLGFQCGIEFQAYIQNTGKYTSLIIEGKRQAGDTI
                     YTYDFYKVRFYNNYTNRVTVYGEHLTPFQLLRRVKSYIYYREKYLKERRTIT"
     gene            4745..4966
                     /locus_tag="SCJ86_000410"
     CDS             4745..4966
                     /locus_tag="SCJ86_000410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_464634.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEG1167998.1"
                     /translation="MNFGQNLYNWFLDNAQSLVLLAIVVIGLFLGFKREFSKLIGFLI
                     IALIAVGLVFNAAGVKDVLLNLFNRIIGA"
     gene            5008..5787
                     /locus_tag="SCJ86_000411"
     CDS             5008..5787
                     /locus_tag="SCJ86_000411"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000678782.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="abortive infection family protein"
                     /protein_id="HEG1167999.1"
                     /translation="MKGFMNMFTNDQFKSLLIKTLERKSSSNYYEGGNEIVSKMKISE
                     VTLDETDDFTYYKGYKKGWNTLCTYLKIRVPFDDLDFFESHKDLITQTASSIYDKQGD
                     NVLVDTILVPLPENYEVINFSQLKISDVVSQAIEDAESFMSNGEYQRAFDRVHTAFHG
                     YLIEILKKYEITVPRDENLSKLYSRIQQLIEKEIQPTELADIVKTTIRSSNGMISSLN
                     EARNRHSLAHPNTNIIGKREAKLIIGISSTVTDYISGYLDK"
     gene            5854..6348
                     /locus_tag="SCJ86_000412"
     CDS             5854..6348
                     /locus_tag="SCJ86_000412"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000870467.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antirestriction protein ArdA"
                     /protein_id="HEG1168000.1"
                     /translation="MKVYVANLGRYNEGELVGAWFTPPIDEEEMAERIGLNEDYEEYA
                     IHDFELPFDVDEYTPISEINRLCEAIQEIEGTPIYNELKEIQGMWFSSLEELLENKED
                     IHCYSDCDSMEDVARYYVEETGQLGEVPSNLQNYIDYQSLGRDMEIEGNYLVTSHGVF
                     EYCQ"
     gene            6404..7042
                     /locus_tag="SCJ86_000413"
     CDS             6404..7042
                     /locus_tag="SCJ86_000413"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000248477.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEG1168001.1"
                     /translation="MVNVFENLKLLKLDMKDKGWVIDSFYFRYKQQNYIVLVKLFEKE
                     EKVPEYALLKLEFLKENDFSDMLAVYANSVKLYTDTKTIREYFGIEYSSNLGDVLFQF
                     SQTLARFIPTEVSEKKNEDQKEAMCFSLSQSDSEDPRKKYCFSVRRNPLKGNGELGKR
                     SPFNDNKTRLYRPSLYERLGSDTNLSFRYSMNPDDEETDEGIIAKWTKNKIE"
     gene            7114..7509
                     /locus_tag="SCJ86_000414"
     CDS             7114..7509
                     /locus_tag="SCJ86_000414"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_464632.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein"
                     /protein_id="HEG1168002.1"
                     /translation="MKKIRSYTSIWSVEKVIYAINDLQLPFPITFTQMTWFIVSLFVV
                     ILFGSLPPLSLIDGALLKYLGIPIVLTGFMCQKTFDGKKPYGFLKSIITYALRPKVTY
                     AGKQIRLKKETMNESITIVRSEQLDGISD"
     gene            7493..9946
                     /locus_tag="SCJ86_000415"
     CDS             7493..9946
                     /locus_tag="SCJ86_000415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000331166.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HEG1168003.1"
                     /translation="MAYPIKYMEDNLVFNHDGEVFAYYELIPYNYSFLSSDEKIQVHD
                     NFCQLIAQNRDGKIHALQLATESSIRATQEQSKKEITGRLKEIAYEKIDEQTEALISM
                     IGDNQVDYRFFIGFKLYLNEQEVSVKNVGKEIQKAFAEFFHGVNHKLMGDFVSMPMAE
                     IERFSKMEQLLESKLSRRFQIRKLEKDDFGYIIEHLYGQTGVPFDDYDYHLPKRYEDK
                     KVLVKKYDILKPTRCLIEENQRYLRIEQEDTTTYVAYFTINSIVGELDFPNSEIFYYQ
                     QQQFTFPVDTSMNVEIVANKKALSTVRNKKKELKDLDSHAWESDNETSTNVVDALESV
                     DELESNLDQTKESMYKLSYVVRVSAKNLDELKQRCDEVKDFYDDLSIKLVRPFGDMLG
                     LHNEFIPSSKRYINDYIQYVTSDFLAGLGFGATQMIGENSGIYVGYNLDTGRNIYLQP
                     SLASQGIKGSVTNALASAFIGSLGGGKSFSNNMLVYYSVLFGGQATIVDPKAERGEWK
                     ETLPDIAHEINIVNLTSENDNKGLLDPYVILKLPKDSESLAIDILTFLTGISSRDSEK
                     FPVLRKAIRSVTQSKQRGLLLVIDELRKEDTPISNSIADHIESFVDYDFAHLLFSDGS
                     VTQSISLEKQLNIIQVADLVLPDSNSSFEEYTTMELLSVAMLIVISTFALDFIHSDRS
                     IFKIVDLDEAWSFLQVAQGKALSMRLVRAGRAMNAGVYFVTQNADDLLDEKMKNNIGL
                     KFAFRSTDLVEIKKTLEFFGVDKEDENNQKRLRELENGQCLVSDIYGRVGVMQFHPIF
                     EELLHAFDTRPPVRNEVEE"
     gene            9943..11970
                     /locus_tag="SCJ86_000416"
     CDS             9943..11970
                     /locus_tag="SCJ86_000416"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000192391.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEG1168004.1"
                     /translation="MTQKQKKFLHYSWITLLCVGVILLLLGTLGNAVQATGLVDETID
                     TSNEYSKYGLNQYQLDYYVDNSWGWLPWNWSDGIGQSVMYGLYAITNFIWTISLYLSN
                     ATGYLVKEAYALDFISQTSQAIGENMQTIAGITPNGFSSSGFYVGFLLLFILIVGIYV
                     AYTGLVKRETTKVIRAVLNFVVVFILSASFIAYAPDYIAKINDFSKDVSTASLNIGTK
                     IVLPNTDSQGKDSVDMIRDSLFSVQVKQPWLLLQYGSTDVEALGEERVESLLSTSPDT
                     NNGEDRENVVIEEIEDHDNTYLTLPKTISRLGTVFFLFVFNIGISIFVFLLTGIMIFS
                     QVLFIIFAMFLPISFLLSMLPSFDGMGRHAITKLFNVIMTRAGITLIITVAFSISTML
                     YSLSASSPFFMIMFLQIVTFAGIYFKLGDLMSLFTLQSNDSQNMGRQVFRRPRQMMNR
                     QTRRFQRSMSKIFRGKAGSTKQKTMTTKQNQTNKANHTRQNERTTPKKETALSNVKKQ
                     QGGIGQRLGEKTAHVLDTKNRLVNKRKQAKQQIQATPTHVKYALHQGKENVTHNVTDF
                     KQSMADTKAQRKQQRMNQQEQKRKNIAQKRLELDKAKEKRRKENPSSPVQNNQQHVRK
                     RQQTVTPVLSQNTKRENHQRPLKKEQKQKTPPKQFNQRKLNKRTDKRGQQT"
     gene            11967..12989
                     /locus_tag="SCJ86_000417"
     CDS             11967..12989
                     /locus_tag="SCJ86_000417"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000768374.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional lysozyme/C40 family peptidase"
                     /protein_id="HEG1168005.1"
                     /translation="MKLKITVICSVVFSLFFFLLLFLVIFFADDTDSGENNKDSSIPQ
                     GGVTVSPEVLAHRPLIEKYGKEYGIEDYVSYILAIMQVESGGTAEDVMQSSESLGLPP
                     NSLSTEESIKQGVKYFSELLTSAEQQGVDIDSVIQSYNYGGGFLNYVRSHGKKYTYEL
                     AEQFSKEKSGGQKADYPNPIAIPVNGGWRYNYGNQFYVQLVSQYLTDTSPTEFDDETV
                     QVIMDEALKYEGFPYVFGGASPTTSFDCSGLIQWVYDKAGISLPRVAQDQYDATQEIS
                     MEEAQAGDLIFFHSTYNAGTYVTHVAIYLEGNRFYHAGDPIGYGDLSSRYWQDHLIGA
                     RRVIHN"
     gene            13006..13935
                     /locus_tag="SCJ86_000418"
     CDS             13006..13935
                     /locus_tag="SCJ86_000418"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017650613.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein"
                     /protein_id="HEG1168006.1"
                     /translation="MIIKIERKEKKEKPKKSKKPSKQRKVPMIKVGTHKKLTFVLWVL
                     LIGSVGFGIYKNFTAIDTHTVRETEIIKQQIVDTNQVESFVKSFAKDYFSWQQSQEAI
                     DKRNEKLTHYLTEELQVLNEEMIRKDIPTSSSVNDIQVWQVSQVNENTFEVLFSVEQV
                     ITEDKDKETISSSFHVVVHIDESDNMVIIKNPTMSKKPQKSDYQPKQLESDHTVDTET
                     MDEITSFLETFFQLYPTATEKELTYYVSNHVLPMINKEYVFEELVNPIFTRKDNQVIV
                     NVAVKYLDQETKATQISQFELILEKQDNWKIVK"
     gene            14180..14296
                     /locus_tag="SCJ86_000419"
     CDS             14180..14296
                     /locus_tag="SCJ86_000419"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001814923.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetracycline resistance determinant leader
                     peptide"
                     /protein_id="HEG1168007.1"
                     /translation="MILKYPENICMLCIPMVMHKNPSDKSIYHWDFYALLGF"
     gene            14312..15115
                     /locus_tag="SCJ86_000420"
     CDS             14312..15115
                     /locus_tag="SCJ86_000420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017644183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTP-binding protein"
                     /protein_id="HEG1168008.1"
                     /translation="MKIINIGVLAHVDAGKTTLTESLLYNSGAITELGSVDKGTTRTD
                     NTLLERQRGITIQTGITSFQWENTKVNIIDTPGHMDFLAEVYRSLSVLDGAILLISAK
                     DGVQAQTRILFHALRKMGIPTIFFINKIDQNGIDLSTVYQDIKEKLSAEIVIKQKVEL
                     YPNMCVTNFTESEQWDTVIEGNDDLLEKYMSGKSLEALELEQEESIRFQNCSLFPLYH
                     GSAKSNIGIDNLIEVITNKFYSSTHRGPSELCGNVFKIEYTKKRQRLAY"
     gene            15350..15586
                     /locus_tag="SCJ86_000421"
     CDS             15350..15586
                     /locus_tag="SCJ86_000421"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001159903.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEG1168009.1"
                     /translation="MQFLEPKNKNAKSVDWEISEQVRVIVKQYAEYAERTESEAVDEF
                     LLNILDDKKFIEWIANKRSNKRIVEKMGIKDRVG"
     gene            15593..17545
                     /locus_tag="SCJ86_000422"
     CDS             15593..17545
                     /locus_tag="SCJ86_000422"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017431446.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="insertion element protein"
                     /protein_id="HEG1168010.1"
                     /translation="MTKLKRLATKEDEIVDVKIPISNEELKDRAKQYDLLTPKRFATR
                     YNKMLFLPTSFKWNGSEYPIQYNYCINPFCCNFGKEQHKFKDVKGKPSRYKMTGSSKD
                     KGHKGMYCNDNPIGRGVSQNCTVTPLSNWSVVEEIKRLIEINSIQDVEPDYQFHKEGC
                     SEEESTPFNEPKQFYKRGKSRGKSQRYQCKACKKFTNVLPKREETTTYHQQKNTILPM
                     FAKMVVGRVSVSRTCDILGIGVGTYYHKLEWLYRRCLEFLERYETQPLQTKKFNEMWL
                     NTDKMHYYLNNVRKKGQGSKKYTGFEDLNMQTYIVVSAEVLSRYVFRSDVAYDWNISM
                     DELNEDTRKFKEDHLNTFSRKNDRLDWSYYPQEPSANDSENRNAYLHELGKITNRSRF
                     VDGLNVDAPYTTTAHYWLIKQMVNADEWRMISDDDFSIRNAFYRVFTKELRLSDAHHF
                     ICQVNKTKSRKQCLKEFGQAKAELLDWGDIRGFKTKFLRTLASHYLTELLTSHQFHEE
                     AISKDGERYRKYADNPIKHPLATKDKGFYSVDCRTDLSALEPNEIAKMLLNVNDHSTN
                     SFIQQIRRYISSLERPLTTARGDKKSYIYANFNPKYAQFAITILRTYYNFCRPFKSAD
                     KKVLTPAQRLGITDKQFDWKDIIYFK"
     gene            complement(17617..18114)
                     /gene="dfrG"
                     /locus_tag="SCJ86_000423"
     CDS             complement(17617..18114)
                     /gene="dfrG"
                     /locus_tag="SCJ86_000423"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000868795.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trimethoprim-resistant dihydrofolate reductase
                     DfrG"
                     /protein_id="HEG1168011.1"
                     /translation="MKVSLIAAMDKNRVIGKENDIPWRIPKDWEYVKNTTKGHPIILG
                     RKNLESIGRALPDRRNIILTRDKGFTFNGCEIVHSIEDVFELCKNEEEIFIFGGEQIY
                     NLFFPYVEKMYITKIHHEFEGDTFFPEVNYEEWNEVFAQKGIKNDKNPYNYYFHVYER
                     KNLLS"
     gene            18476..19516
                     /locus_tag="SCJ86_000424"
     CDS             18476..19516
                     /locus_tag="SCJ86_000424"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000288739.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor G"
                     /protein_id="HEG1168012.1"
                     /translation="MYTSINGELCKIDRAYSGEIVILQNEFLKLNSVLGDTKLLPQRK
                     KIENPHPLLQTTVEPSKPEQREMLLDALLEISDSDPLLRYYVDSTTHEIILSFLGKVQ
                     MEVISALLQEKYHVEIELKEPTVIYMERPLKNAEYTIHIEVPPNPFWASIGLSVSPLP
                     LGSGMQYESSVSLGYLNQSFQNAVMEGIRYGCEQGLYGWNVTDCKICFKYGLYYSPVS
                     TPADFRMLTPIVLEQAFRKAGTELLEPYLSFKVYAPQEYLSRAYNDAPKYCANIVNTQ
                     LKNNEVIIIGEIPARCIQDYRNDLTFFTNGLSVCLAELKGYQVTTGEPVCQTRRLNSR
                     IDKVRYMFNKIT"
     gene            19614..19802
                     /locus_tag="SCJ86_000425"
     CDS             19614..19802
                     /locus_tag="SCJ86_000425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011988843.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine-rich KTR domain-containing protein"
                     /protein_id="HEG1168013.1"
                     /translation="MINYKWILCPICGNKTRLRIRVDTILKNFPLYCPKCKNETLINV
                     QKMNIITIKEPDAKTQSR"
     gene            complement(19860..20213)
                     /locus_tag="SCJ86_000426"
     CDS             complement(19860..20213)
                     /locus_tag="SCJ86_000426"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001227349.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HEG1168014.1"
                     /translation="MRKKEDKYDFRAVGLAIKEARMKRGLTREQVGTMIEIDPRYLTN
                     IENKGQHPSTQVLYDLVSLLHVSIDEFFLPTDNLIKSTRRLQVEKYMDSFTDKELSLM
                     EALAKGINEARNIEG"
     gene            20744..21166
                     /locus_tag="SCJ86_000427"
     CDS             20744..21166
                     /locus_tag="SCJ86_000427"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006733729.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HEG1168015.1"
                     /translation="MKPSSFQETIENQFDYICKQVIEDERKDYFKHLDRLSAKEVSFS
                     DIDNYLVNSFSTVDTYVTDFQLFKLFGLRINVESDLLSEALRHLSEKKRNIILLHYFM
                     DMSDVEIAELLNLNRSTVYRHRISGLAMIKEFMKECKE"
     gene            21163..21393
                     /locus_tag="SCJ86_000428"
     CDS             21163..21393
                     /locus_tag="SCJ86_000428"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002411750.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HEG1168016.1"
                     /translation="MKTAYPRVPFPLIVKATDGDVEAINQIVKHYRGYTSKRSLRRMT
                     DEYGNSHMVIDETLRGRMETKLISKILSFEIK"
     gene            21891..22091
                     /locus_tag="SCJ86_000429"
     CDS             21891..22091
                     /locus_tag="SCJ86_000429"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_008475635.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3173 domain-containing protein"
                     /protein_id="HEG1168017.1"
                     /translation="MITVTKKDLVELGYGTSFSADIIKKAKELMITKGHIYYQSRKLD
                     RVPREAVEEILGIRFSNENRSE"
     gene            22118..23311
                     /locus_tag="SCJ86_000430"
     CDS             22118..23311
                     /locus_tag="SCJ86_000430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002388733.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HEG1168018.1"
                     /translation="MVKDPIKKAKNGTYYFRANLGYDSKGKKIQKYRSGFTTKKEARE
                     EYSKLLLMKPEELSENKDKMTFKHYIFEIFLPWYKTQVKLRTYENRLPTIKKHFSYFD
                     KMAVSDIEPIDVQNWQLKLSKKCKSSYVRAVQGLFSVAMDRAIVLGITTTNPSKIIGN
                     VKKQKSKIEFWTKEEFEKVISLIYKEDYYQHFLFISLWFLFMTGMRIGEATAIQWEDI
                     DFDSGVLTINKTLYYKNQSNYRFVEPKTKASVRHIVLDKCTLTYLSEWKEVQQNLIQT
                     DFVMSYNGIPTQKHTLSYAIERYAKLAGIHRIRIHGLRHSHASLLISMGENPLIIKDR
                     LGHEDIETTLGTYGHLYPNSNFEVANKLNGAISFKEAKQNYDTSPKNQFTVDYLRNKE
                     MKKVQ"
     gene            complement(23379..24944)
                     /gene="guaA"
                     /locus_tag="SCJ86_000431"
     CDS             complement(23379..24944)
                     /gene="guaA"
                     /locus_tag="SCJ86_000431"
                     /EC_number="6.3.5.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734496.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine-hydrolyzing GMP synthase"
                     /protein_id="HEG1168019.1"
                     /translation="MTNVADMTNVEKIIVLDYGSQYNQLITRRIRDLGVFSELLSHRI
                     TAEEVKAMNPKGIILSGGPNSVYDENAFGIDPEIFNLGIPVLGICYGMQLITYKLGGS
                     VEPAKNREYGKALLEVTSDQAVLFKETPKQQTVWMSHGDLVTKVPEGFETVGTSKDCP
                     IASIQDTTRNFYGIQFHAEVRHSEYGIELLRHFAFDVCHCEGNWTMDNFIDMQVEKIR
                     EQVGDKKVLLGLSGGVDSSVVGVLLQKAIGDQLTSIFVDHGLLRKGEAEQVMESLGGK
                     FGLNIIKVDARDRFLDKLKGVSDPEQKRKIIGNEFVYVFDDEATKLAGEEGVSFLAQG
                     TLYTDVIESGTETAQTIKSHHNVGGLPEDMQFELIEPLNTLFKDEVRELGTQLGMPDS
                     IVWRQPFPGPGLGIRVLGEITEDKLEIVRESDAILREEIAAAGLDRDIWQYFTVLPGI
                     RSVGVMGDGRTYDYTVGIRAVTSIDGMTADFARIPWEVLQKISVRIVNEVAHVNRIVY
                     DITSKPPATVEWE"
     gene            complement(25003..25926)
                     /locus_tag="SCJ86_000432"
     CDS             complement(25003..25926)
                     /locus_tag="SCJ86_000432"
                     /EC_number="2.7.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010750824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I pantothenate kinase"
                     /protein_id="HEG1168020.1"
                     /translation="MNDPMNYYRVAREEWREFYRNGQAPLTQDELDNIKSLNDRISMQ
                     DVRDIYVPLAHLIYLYMKEFESLFLSKGLFLHKYVEVPPFIIGIAGSVAVGKSTTARL
                     LQTILSRTFKRRNVQLITTDGFLYPNRILKERGIMDRKGFPESYDMEMLIDFLNRVKS
                     GQEEIKVPVYSHDVYDIIPDKYELIQQPDILIVEGINTLQLPANQQIYVSDFFDFSVY
                     VDADPVLIEKWYLDRFGALLDTAFQNPENYYYRFAIGDREEAFKMAKDVWKNVNLKNL
                     QEYILPTRGRADIILHKTENHIIDSIFMRKY"
     gene            26122..26727
                     /locus_tag="SCJ86_000433"
     CDS             26122..26727
                     /locus_tag="SCJ86_000433"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307304.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HEG1168021.1"
                     /translation="MTNHYYSERPDTAHDFEQWAFELKGRTFQFVTDSGVFSRETVDY
                     GSRVLIDAFDWEELPDEGKILDVGCGYGPIGLALAFATQRFVEMVDINERAVSLAQGN
                     AKRNQIEHVDIHQSNIYEAVHEETYAAIVSNPPIRAGKKVVHEILTGAYPRLKKGGTL
                     TIVIQKKQGAPSAQKKMEETFGNAEIVTKDKGYYIIKSVKE"
     gene            27035..28051
                     /locus_tag="SCJ86_000434"
     CDS             27035..28051
                     /locus_tag="SCJ86_000434"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010750821.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="substrate-binding domain-containing protein"
                     /protein_id="HEG1168022.1"
                     /translation="MKLTIRDIAEMAGVSVTTVSQILNNKGSRFSDKTRKKVLSIVNE
                     HHYKPDYFASNVINRHSKTIGMIVPDVTDFFFSKLIEGVESYLNPLGYVIMLCNSRHS
                     QENEIKYLQELSHRSVDGILLATPNILPEKYALDSGFYQKMPIILIDRGLNRRDNGRL
                     IVKEYEGAYQAVSLFIENGHTKIGMLKETTGYYQLEERFNGYRHALKDHGLPFNGKFV
                     EHGELTVQGGYTASKKLLKHKDITAIFCGNDAMAIGCYQAIDELGKKIPEDISVIGFD
                     GLKLSEYMIPQLTTVQQPSFDIGFYAARFLIDTIEFPQRKVPNKVFETKLIIRESVKV
                     LTKG"
     gene            28192..29493
                     /locus_tag="SCJ86_000435"
     CDS             28192..29493
                     /locus_tag="SCJ86_000435"
                     /EC_number="2.4.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019724048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyrimidine-nucleoside phosphorylase"
                     /protein_id="HEG1168023.1"
                     /translation="MRMVDLIEKKRDGNELSKEEIEYIVTNYTNGKIPDYQVSALLMA
                     IFYQDMTNEEITNLTLAIANSGDVIDLSSLEGIKVDKHSTGGVGDTTTLILAPLVASV
                     GVTVAKMSGRGLGYTGGTLDKLEAIPGFQIELSDEEFVRIVNESKVAVIGQSGNLAPA
                     DKKLYALRDVTATVDSLPLIASSIMSKKIAAGADAIVLDVTTGDGAFMKNIEDARRLA
                     KTMTSIGKLANRETVAVISDMSEPLGEAIGNSLEVVEAIETLQGNGPEDLVEMCYALG
                     SQMVVLAGKAKTTDEARTLLQEALESGKALAKFKEMIQNQGGDPAIVEHPERILTARY
                     TMELPAKQSGVVSKIVANELGIAAMMLGAGRKTKEEDIDHAVGLKLHKKIGDTVTKGE
                     SLLTIYSNDEEITSVIELLYKNIEIGESAMKPTLIHDIITE"
     gene            29539..30201
                     /gene="deoC"
                     /locus_tag="SCJ86_000436"
     CDS             29539..30201
                     /gene="deoC"
                     /locus_tag="SCJ86_000436"
                     /EC_number="4.1.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005880846.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="deoxyribose-phosphate aldolase"
                     /protein_id="HEG1168024.1"
                     /translation="MELNRMIDHTILKADASKEAVMQIIEEAKKYHFYSVCINPAWVA
                     LAKEQLQGEPVAVCTVIGFPLGANTPETKAFETTDAINNGADEVDMVINIGALKSKDD
                     QQVQKDIEAVVEAAKDRALVKVIIETSLLDREEIIRACEIAKAAGADFVKTSTGFSTG
                     GAKVEDVRLMRETVGPEMGVKASGGIHNEEEAMAMIEAGATRIGTSAGVAIVSGSTGE
                     GY"
     gene            30222..30620
                     /locus_tag="SCJ86_000437"
     CDS             30222..30620
                     /locus_tag="SCJ86_000437"
                     /EC_number="3.5.4.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytidine deaminase"
                     /protein_id="HEG1168025.1"
                     /translation="MAELKQEWINTAIEALDKAYVPYSHFPVGACLVTESGKIYQGIN
                     IENASFGLTNCAERTAFFKAVSEGEKTFTHLVVAGHTPDPISPCGACRQVMAEFCSPD
                     MPVTLVGDNGVTKATTVRALLPYAFTEKDL"
     gene            30686..31768
                     /locus_tag="SCJ86_000438"
     CDS             30686..31768
                     /locus_tag="SCJ86_000438"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324275.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BMP family ABC transporter substrate-binding
                     protein"
                     /protein_id="HEG1168026.1"
                     /translation="MKKAKLFGLGAVALAAGLFLGACGNNGSTDSSGGKSSSDTTTAA
                     LITDTGGVDDRSFNQSAWEGLEKWGKDQGLSRGNDGFQYFQSSNESDYIPNIDQALNA
                     GFKTIFGIGYKLKPAIQEQATNNTGTNFVIIDDVIDGLDNVVSATFKDNEASYLAGVA
                     AAYTTETNVVGFIGGVKGEVIDRFDAGFKAGVDAGAKELGKEIKVLNQYAGDFSAPDK
                     GRSIAQGMYAQNADIIFHASGGTGNGVFQEAKSLNESGDKKVWVIGVDRDQSDEGEYT
                     LNGEKKNFTLTSTLKAVGTVVEDLAQKSADGKFPGGEHTVYGLKEDGVGLTEGQLSDE
                     AKKAVDEAKEKIISGDVKVPETPEEN"
     gene            31980..33542
                     /locus_tag="SCJ86_000439"
     CDS             31980..33542
                     /locus_tag="SCJ86_000439"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005880840.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEG1168027.1"
                     /translation="MVQENIVIEMQNITKEFGTFKANDNINLQVKAGEIHALLGENGA
                     GKSTLMNVLSGLLEPTSGKILMRGKEVKITSPTKANQLGIGMVHQHFMLVDAFTVTEN
                     IVLGSEPSRAGMLDHKKARKEIQKVSEQYGLSVNPDAYVRDISVGMEQRVEILKTLYR
                     GADVLIFDEPTAVLTPQEIDELIVIMKELVKEGKSIILITHKLDEIKAVADRCTVIRR
                     GKGIGTVNVKDVTSQQLADMMVGRTVSFKTMKKEAKPQEVVLSIENLVVKENRGLEAV
                     KNLNLEVRAGEVLGIAGIDGNGQSELIQALTGLRKAESGHIKLKGEDITNKKPRKITE
                     HGVGHVPEDRHKYGLVLDMTLSENIALQTYHQKPYSKNGMLNYSVMNEHARELIEEYD
                     VRTTNELVPAKALSGGNQQKAIIAREVDRDPDLLIVANPTRGLDVGAIEFIHKRLIEQ
                     RDKYKAVLLISFELEEILNVSDRIAVIHEGEIVGIVDPKETSENELGLLMAGYTLEEA
                     RKELEKGAGAHE"
     gene            33535..34668
                     /locus_tag="SCJ86_000440"
     CDS             33535..34668
                     /locus_tag="SCJ86_000440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005880838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEG1168028.1"
                     /translation="MNDRSEKFRNLLVPVLSVVLGLILGAILMAAFGFQPIQGYSSML
                     DAALGSQRGIGETLRQATPLIFTALGFSVANSAGFFNIGLSGQALCGWIVSVWVALAF
                     PDLPKVVLLPLCVILGALAGAMAAAVPGLLRAFFGTSEVIVTIMMNYILLYVSTFALH
                     EVMPESYRSSLDSSNMISQNASLRLPWLTQAFGGSRVNSGLFLAIIALIFVWLLMKKT
                     TLGFEIRSVGLNPFASEYAGMSSKRTIVLSMVISGALAGLGGVVEGLGTYQNFFVQTT
                     SLSIGFDGMAVSLLGSGSAIGILLSAILFSILKIGGLGMQTGAGVPFEIVNVSIALII
                     FFVGINFLIRFVMAKVFQGKKQEEIVATIESKPTNQNHEGGEL"
     gene            34668..35624
                     /locus_tag="SCJ86_000441"
     CDS             34668..35624
                     /locus_tag="SCJ86_000441"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005880836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEG1168029.1"
                     /translation="MSTIELIALILTSTLVYSTPLILTSLGGTFSERSGIVNVGLEGI
                     MVMGAFSSIVFNLTMSPSLGSMTPWIAILVGGVVGILFSLIHAVATVSLRADHIISGT
                     VINLMAPALGVFLIKAWYGKGQTDNITQNLGYFSFPGLADIPVIGPIFFKNTFLPAYC
                     AILIAVLAWVIIFKTKFGLRLRSVGENPQAADTLGINVYAMRYSGVLISGLLGGMGGA
                     VFAQSISGNFSAGTIVGQGFISMAAMIFGKWNPLGAMGASLFFGFAQSLSIIGAQLPV
                     ISSIPPVLLQIAPYLLTIIVLVVFLGKASGPKANGKNYIKSK"
     gene            35723..36889
                     /gene="deoB"
                     /locus_tag="SCJ86_000442"
     CDS             35723..36889
                     /gene="deoB"
                     /locus_tag="SCJ86_000442"
                     /EC_number="5.4.2.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301034.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphopentomutase"
                     /protein_id="HEG1168030.1"
                     /translation="MFKRVHLIVMDSVGIGEAPDADKFGDVGSDTLGHIAKEAGLNVP
                     HLEQLGLGTIRPLEGVESVKDHQGYATKLEEVSVGKDTMTGHWEIMGLNIKTPFRVFP
                     NGFPDDLIKQIEEFSGRKVVCNKPYSGTAVIDDYGEHQMKTGDLIVYTSADPVLQIAA
                     HEEIIPLEELYRICQFVRDITKDDPYMIGRIIARPYVGEPGDFTRTSNRHDYALNPFG
                     HTVLDSLKEAGKDVIAVGKINDIFNGQGITESVRTKSNMDGVDQLLKVMKNDFSGISF
                     TNLVDFDALYGHRRDVVGYAHAIEEFDLRIPELLEAMEEDDLLMITADHGNDPTFTGT
                     DHTREYVPLLVYSKKMKEQGALPQGYYSDISATIAENFSVSSTENGESFLKLLK"
     gene            36913..37731
                     /locus_tag="SCJ86_000443"
     CDS             36913..37731
                     /locus_tag="SCJ86_000443"
                     /EC_number="2.4.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289198.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="purine-nucleoside phosphorylase"
                     /protein_id="HEG1168031.1"
                     /translation="MTKLSEMLQETTQFILDKGVKEIDFGLILGSGLGELADEIEDAV
                     MISYDEIPFFPVSTVVGHAGKLVYGSLAGKKVLAMQGRFHYYEGHSMQTVTYPVRVMA
                     GLGAHSVIVTNACGGVNESFVPGDLMLITDHINFTGENPLIGPNEDAMGPRFPDMSQA
                     YTPDYQATAKAAAEKLGLDLKEGVYMGYSGPTYETPAEIRMSRTIGADAVGMSTVPEV
                     IVAAHSGLKVLGISCITNLAAGMQANLSHEEVVETTQRVKQSFKALVKETLTLL"
     gene            37748..38455
                     /gene="deoD"
                     /locus_tag="SCJ86_000444"
     CDS             37748..38455
                     /gene="deoD"
                     /locus_tag="SCJ86_000444"
                     /EC_number="2.4.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324276.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="purine-nucleoside phosphorylase"
                     /protein_id="HEG1168032.1"
                     /translation="MSVHIEAKEGEIADKILLPGDPLRAKYIAETFLEDPVCYNQVRG
                     MLGFTGKYKGERISVQGTGMGMPSATIYAHELIQSYGVKKLIRVGTCGALSKDVHVRD
                     LVLAQGAATSSSMIEKNFQAFHFPPISDFNLLLKAYEIAKEKGYTVHVGNVLSEDSFY
                     KDDLTETFQLAELGVLGVEMEAAALYYLGAKYHVQTLSLMTVSDHLITGEETTAAERQ
                     STFNEMIEVGLETAIAE"
     gene            complement(38753..39439)
                     /gene="gpmA"
                     /locus_tag="SCJ86_000445"
     CDS             complement(38753..39439)
                     /gene="gpmA"
                     /locus_tag="SCJ86_000445"
                     /EC_number="5.4.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2,3-diphosphoglycerate-dependent
                     phosphoglycerate mutase"
                     /protein_id="HEG1168033.1"
                     /translation="MPKLVFSRHGLSEWNALNQFTGWADVNLAPEGIEEAKEGGRKIK
                     EAGIEFDVAYTSVLTRAIKTCNLILEYSDQLWVPQIKSWRLNERHYGKLQGLNKKETA
                     EKYGDEQVHIWRRSYDTLPPLMEETDEGSAANDRRYAMLDKRDIPGGENLKVTLERAL
                     PFWQDEIAPALLDNKTVLVAAHGNSLRALAKHIEGISDEDIMDLEIPTGQPLVYELND
                     DLTVAKKYYL"
     gene            complement(39611..40192)
                     /locus_tag="SCJ86_000446"
     CDS             complement(39611..40192)
                     /locus_tag="SCJ86_000446"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323316.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HEG1168034.1"
                     /translation="MKIIGYARTTINDVDLTTQVDQLSDFGCQEIFHETYEVEQNDST
                     AVHLESVIADMQQGDALVICQLHRLGKSTRQLTELTQLFKDRGLHLISLDEGIDTREM
                     GKIYFQLMENLANMECALIKERTLVGLDEARKKGKIGGRPKIDAKTIKKIRRLYYEKK
                     ETIQFISSKCGVSVGTCYKYINLPEKEIEQLCI"
     gene            complement(40325..41008)
                     /gene="rpiA"
                     /locus_tag="SCJ86_000447"
     CDS             complement(40325..41008)
                     /gene="rpiA"
                     /locus_tag="SCJ86_000447"
                     /EC_number="5.3.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341939.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribose-5-phosphate isomerase RpiA"
                     /protein_id="HEG1168035.1"
                     /translation="MNLKQLAGIEAAKFVKDGMIVGLGTGSTAYYMVEEIGRRMREEG
                     LRITGVTTSNATKEQAEKLGIPLKSIDEVPVVDLTIDGADEISADFQGIKGGGAALLF
                     EKIVATYSKETIWIVDSSKLVDKLGKFPLPVEVIPYGSQQLLHIFEEKRFQPVLRTDE
                     NGEVLTTDGGHYIIDLHLEVIEQPESLATYLDELVGVVEHGLFLNMVSKVVVGSEKGV
                     EILDAIRRK"
     gene            41327..41740
                     /gene="rpsL"
                     /locus_tag="SCJ86_000448"
     CDS             41327..41740
                     /gene="rpsL"
                     /locus_tag="SCJ86_000448"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002356190.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S12"
                     /protein_id="HEG1168036.1"
                     /translation="MPTINQLVRKPRKSKVEKSNSPALNKGYNSFKKTQTNVNSPQKR
                     GVATRVGTMTPKKPNSALRKYARVRLSNLIEVTAYIPGIGHNLQEHSVVLLRGGRVKD
                     LPGVRYHIVRGALDTAGVNDRKQSRSKYGTKKPKA"
     gene            41824..42294
                     /gene="rpsG"
                     /locus_tag="SCJ86_000449"
     CDS             41824..42294
                     /gene="rpsG"
                     /locus_tag="SCJ86_000449"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_466177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S7"
                     /protein_id="HEG1168037.1"
                     /translation="MPRKGPVAKRDVLPDPIYNSKLVTRLINRVMVDGKRGIAANIIY
                     NAFDLIKESTGNDPLEVFEQAMKNVQPVLEVKARRVGGSNYQVPVEVRPERRVTLGLR
                     WVVNYARLRGEHTMEERLAKEIMDAANNTGASVKKREDTHKMADANRAFAHYRW"
     gene            42388..44475
                     /gene="fusA"
                     /locus_tag="SCJ86_000450"
     CDS             42388..44475
                     /gene="fusA"
                     /locus_tag="SCJ86_000450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294317.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor G"
                     /protein_id="HEG1168038.1"
                     /translation="MTREFSLQNTRNIGIMAHIDAGKTTATERILYYTGKIHKIGETH
                     EGASQMDWMEQEQERGITITSAATTAQWKGHRINIIDTPGHVDFTVEVERSLRVLDGA
                     VTVLDAQSGVEPQTETVWRQATTYGVPRIVFANKMDKIGADFLYSVSTLHDRLQANAH
                     PIQLPIGAEDDFTGIIDLVTMKAEMYTNDLGTEIEETEIPEEYRELAEEWREKLVEAV
                     AETDEELTLKYLEGEEITEAELKEGIRRATVNVEFYPVLCGSAFKNKGVQLLLDAVLD
                     YLPSPLDIPAIKGIDPKTDEEVERPADDSAPFSALAFKVMTDPFVGRLTFFRVYSGVL
                     QSGSYVQNATKGKRERVGRILQMHANSRSEISEVYAGDIAAAVGLKDTTTGDTLCDEK
                     SLVILESMEFPEPVIQVAIEPKSKADQDKMGVALQKLSEEDPTFRAETNVETGETIIA
                     GMGELHLDIIVDRMRREFKVEANVGAPQVSYRETFRAGTQAEGKFVRQSGGKGQYGHV
                     WIEFTPNEEGAGFEFENAIVGGVVPREYIPAVETGLKDAMENGVLAGYPLVDIKAKLY
                     DGSYHDVDSNETAFRVAASMALRAAAKKANPVILEPIMAVEVVIPEDYLGDVMGHVTA
                     RRGRVEGMEARAGGQQVVRAMVPLAEMFGYATTLRSATQGRGTFTMTFDHYEDVPKSV
                     QEEIIKKNGGKAE"
     gene            44647..45834
                     /gene="tuf"
                     /locus_tag="SCJ86_000451"
     CDS             44647..45834
                     /gene="tuf"
                     /locus_tag="SCJ86_000451"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003127959.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor Tu"
                     /protein_id="HEG1168039.1"
                     /translation="MAKEKFDRSKPHVNIGTIGHVDHGKTTLTAAITTVLSKKNGGQA
                     MAYDQIDGAPEERERGITISTAHVEYETDTRHYAHVDCPGHADYVKNMITGAAQMDGA
                     ILVVSAADGPMPQTREHILLSRQVGVPYIVVFLNKVDMVDDEELLELVEMEVRDLLTE
                     YEFPGDDVPVVAGSALKALEGDASYEEKILELMAAVDEYIPTPERDNDKPFMMPVEDV
                     FSITGRGTVATGRVERGQVRVGDEVEVVGIAEETSKTTVTGVEMFRKLLDYAEAGDNI
                     GALLRGVAREDIQRGQVLAKPGTITPHTKFSAEVYVLTKEEGGRHTPFFTNYRPQFYF
                     RTTDVTGVVELPEGTEMVMPGDNVTMEVELIHPIAIENGTKFSIREGGRTVGAGVVTE
                     IKA"
     gene            46241..46549
                     /gene="rpsJ"
                     /locus_tag="SCJ86_000452"
     CDS             46241..46549
                     /gene="rpsJ"
                     /locus_tag="SCJ86_000452"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003046044.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S10"
                     /protein_id="HEG1168040.1"
                     /translation="MAKQKIRIRLKAYEHRILDQSADKIVETAKRTGASVSGPIPLPT
                     ERSLYTIIRATHKYKDSREQFEMRTHKRLIDIVNPTPKTVDALMKLDLPSGVNIEIKL
                     "
     gene            46580..47209
                     /gene="rplC"
                     /locus_tag="SCJ86_000453"
     CDS             46580..47209
                     /gene="rplC"
                     /locus_tag="SCJ86_000453"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002351833.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L3"
                     /protein_id="HEG1168041.1"
                     /translation="MTKGILGKKVGMTQIFTESGELIPVTVVEATPNVVLQVKTMETD
                     GYEAIQVGYQDMREVLSNKPAKGHVAKANTAPKRFIREFKNVELGEYEVGKEITVDVF
                     QAGDIIDVTGTTKGKGFQGVIKRHGQSRGPMAHGSRYHRRPGSMGPVAPNRVFKNKRL
                     AGRMGGNRVTIQNLEVVRVDAERNVILIKGNVPGAKKSLITIKSAVKAK"
     gene            47237..47860
                     /gene="rplD"
                     /locus_tag="SCJ86_000454"
     CDS             47237..47860
                     /gene="rplD"
                     /locus_tag="SCJ86_000454"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002356200.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L4"
                     /protein_id="HEG1168042.1"
                     /translation="MPNVALFKQDGSQNGEITLNEEIFGIEPNESVVYDAIVMQRASL
                     RQGTHAVKNRSAVRGGGRKPWRQKGTGRARQGSIRSPQWRGGGVVFGPTPRSYSYKLP
                     KKVRRLAMKSVLSEKVAENNLVAIEGLNFDAPKTKEFKQVLANLSIDSKVLVVLEKGN
                     DFAALSARNLPNVSVVTSDNVSVLDVVSNTKVLATQTALTQIEEVLA"
     gene            47860..48150
                     /gene="rplW"
                     /locus_tag="SCJ86_000455"
     CDS             47860..48150
                     /gene="rplW"
                     /locus_tag="SCJ86_000455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L23"
                     /protein_id="HEG1168043.1"
                     /translation="MNLLDVIKRPVITEKSMLAMDDKKYTFEVDTRANKTLVKQAVEA
                     AFDVKVKNVNIVNVRAKFKRMGKYAGYTKKRRKAIVTLTEDSKEIQLFEAAE"
     gene            48189..49022
                     /gene="rplB"
                     /locus_tag="SCJ86_000456"
     CDS             48189..49022
                     /gene="rplB"
                     /locus_tag="SCJ86_000456"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010756442.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L2"
                     /protein_id="HEG1168044.1"
                     /translation="MAIKKYKPTSNGRRNMTGSDFAEITTSTPEKSLLQPLKNHAGRN
                     NNGRITVRHQGGGHKRQYRVIDFKRNKDNVVATVQTIEYDPNRSANIALVHYEDGVKA
                     YILAPKGLEVGMKLVSGPEADIKVGNALPLENIPVGTVIHNIEMKPGKGGQLIRSAGT
                     SAQVLGKEGKYVLIRLNSGEVRMILAACRATIGSVGNEQHELINIGKAGRSRWMRKRP
                     TVRGSVMNPNDHPHGGGEGKAPIGRKAPVSPWGQPALGYKTRNKKAKSDKLIVRRRKN
                     K"
     gene            49065..49343
                     /gene="rpsS"
                     /locus_tag="SCJ86_000457"
     CDS             49065..49343
                     /gene="rpsS"
                     /locus_tag="SCJ86_000457"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000533765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S19"
                     /protein_id="HEG1168045.1"
                     /translation="MGRSLKKGPFVDEHLMKKVEAQAGVEKKKVIKTWSRRSTVFPSF
                     VGYTIAVYDGRKHVPVYIQEDMVGHKLGEFAPTRTYRGHAADDKKTKR"
     gene            49366..49713
                     /gene="rplV"
                     /locus_tag="SCJ86_000458"
     CDS             49366..49713
                     /gene="rplV"
                     /locus_tag="SCJ86_000458"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003701308.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L22"
                     /protein_id="HEG1168046.1"
                     /translation="MAEQITSAKATAKTVRTSPRKARLVIDLIRGKSVADAISILKFT
                     PNKSAGIIEKVLMSAVANAENNFDLDVENLVVSEAFVNEGPTMKRFRPRAKGSASPIN
                     KRTSHITVVVSEK"
     gene            49727..50383
                     /gene="rpsC"
                     /locus_tag="SCJ86_000459"
     CDS             49727..50383
                     /gene="rpsC"
                     /locus_tag="SCJ86_000459"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000529931.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S3"
                     /protein_id="HEG1168047.1"
                     /translation="MGQKVHPIGMRVGIIRDWDAKWYAEKEYAEFLHEDLRIRKFIST
                     KLADAAVSTVEIERATGRVNISIHTAKPGMVIGKGGSEVESLRKELNKLTGKRIHINI
                     VEIKKPDLDAKLVGEGIARQLENRVAFRRAQKQAIQRSMRAGAQGIKTQVSGRLNGAD
                     IARSEGYSEGTVPLHTLRADIDYAWEEADTTYGKLGVKVWIYRGEILPTKKNTEKGGK
                     "
     gene            50386..50820
                     /gene="rplP"
                     /locus_tag="SCJ86_000460"
     CDS             50386..50820
                     /gene="rplP"
                     /locus_tag="SCJ86_000460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288661.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L16"
                     /protein_id="HEG1168048.1"
                     /translation="MLVPKRVKHRREFRGKMRGEAKGGKEVAFGEYGLQAVDSHWITN
                     RQIEAARIAMTRYMKRGGKVWIKIFPHKSYTAKAIGVRMGSGKGAPEGWVAPVKRGKI
                     MFEIAGVPEEVAREALRLASHKLPMKTKIVKREEMGGESNEG"
     gene            50810..50998
                     /gene="rpmC"
                     /locus_tag="SCJ86_000461"
     CDS             50810..50998
                     /gene="rpmC"
                     /locus_tag="SCJ86_000461"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288664.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L29"
                     /protein_id="HEG1168049.1"
                     /translation="MKVKEIRELTTAEMLDQEKQLKEELFNLRFQLATGQLENTARIK
                     EVRKSIARIKTVLREQAK"
     gene            51023..51289
                     /gene="rpsQ"
                     /locus_tag="SCJ86_000462"
     CDS             51023..51289
                     /gene="rpsQ"
                     /locus_tag="SCJ86_000462"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016184196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S17"
                     /protein_id="HEG1168050.1"
                     /translation="MTEGRNQRKVYQGRVVSDKMDKTITVAVETKKNHPIYGKRMNYS
                     KKYKVHDENNTAKVGDIVRIMETRPLSATKRFRLLEVVEEAVII"
     gene            51347..51715
                     /gene="rplN"
                     /locus_tag="SCJ86_000463"
     CDS             51347..51715
                     /gene="rplN"
                     /locus_tag="SCJ86_000463"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012727667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L14"
                     /protein_id="HEG1168051.1"
                     /translation="MIQAESRLKVADNSGAREILTIKVLGGSGRKTANIGDVIVATVK
                     QATPGGVVKKGDVVKAVIVRTKSGARRTDGSYIKFDENAAVIIRDDKSPRGTRIFGPV
                     ARELRENNFMKIVSLAPEVL"
     gene            51752..52060
                     /gene="rplX"
                     /locus_tag="SCJ86_000464"
     CDS             51752..52060
                     /gene="rplX"
                     /locus_tag="SCJ86_000464"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002356212.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L24"
                     /protein_id="HEG1168052.1"
                     /translation="MFVKKGDKVKVITGKDKNKEGVVLAAFPKKDKVIVEGVNVVKKH
                     QKPSQAAPQGGIVEMEAPIHVSNVMVVDSTGVAGRVGYKEVDGKKVRVSKKTGEVLDK
                     "
     gene            52088..52627
                     /gene="rplE"
                     /locus_tag="SCJ86_000465"
     CDS             52088..52627
                     /gene="rplE"
                     /locus_tag="SCJ86_000465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_466143.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L5"
                     /protein_id="HEG1168053.1"
                     /translation="MNRLKEKYLKEVTPSLMEKFNYSSVMQTPKVEKIVINMGVGDAV
                     SNAKNLDKAVEELTLITGQKPMITKAKKSIAGFRLREGMPIGAKVTLRGERMYEFLDK
                     LVSVSLPRVRDFHGVSKKAFDGRGNYTLGIKEQLIFPEVDYDLVDKVRGMDIVIVTTA
                     NSDEESRELLTQLGMPFQK"
     gene            52646..52831
                     /locus_tag="SCJ86_000466"
     CDS             52646..52831
                     /locus_tag="SCJ86_000466"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011245027.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type Z 30S ribosomal protein S14"
                     /protein_id="HEG1168054.1"
                     /translation="MAKKSMIAKNKRPAKFSTQAYTRCERCGRPHSVYRKFHLCRICF
                     RELAYKGQIPGVKKASW"
     gene            52867..53265
                     /gene="rpsH"
                     /locus_tag="SCJ86_000467"
     CDS             52867..53265
                     /gene="rpsH"
                     /locus_tag="SCJ86_000467"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010742763.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S8"
                     /protein_id="HEG1168055.1"
                     /translation="MVMTDPIADFLTRIRNANMVKHESLEVPASKIKRDIAEILKREG
                     FVRDVEYIEDDKQGVIRVFLKFGKNGERVITNLKRISKPGLRAYVKADEVPKVLNGLG
                     IAIISTSEGVVTDKEAREKNIGGEVIAYVW"
     gene            53297..53833
                     /gene="rplF"
                     /locus_tag="SCJ86_000468"
     CDS             53297..53833
                     /gene="rplF"
                     /locus_tag="SCJ86_000468"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016250300.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L6"
                     /protein_id="HEG1168056.1"
                     /translation="MSRIGNKVVVIPEGVTVTQDGNNVTVKGPKGELTRTFSADIKMN
                     IEGNEVTFTRPNDSKEMKTIHGTTRANFNNMVVGVSEGFQKALELIGVGYRAQLQGSK
                     LVLNVGYSHPVEITPPAGVTVEVPANTQVIVKGANKEEVGELAANIRGTRPPEPYKGK
                     GIRYVGEYVRRKEGKTGK"
     gene            53994..54350
                     /gene="rplR"
                     /locus_tag="SCJ86_000469"
     CDS             53994..54350
                     /gene="rplR"
                     /locus_tag="SCJ86_000469"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000628816.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L18"
                     /protein_id="HEG1168057.1"
                     /translation="MITKPDKNKTRQKRHRRVRNNISGTAERPRLNVFRSNKNIYAQI
                     IDDVAGVTLASASTLDKEISGGTKTEAAQAVGKLVAERAAEKGIKVVVFDRGGYLYHG
                     RVQALAEAARENGLEF"
     gene            54371..54871
                     /gene="rpsE"
                     /locus_tag="SCJ86_000470"
     CDS             54371..54871
                     /gene="rpsE"
                     /locus_tag="SCJ86_000470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288681.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S5"
                     /protein_id="HEG1168058.1"
                     /translation="MVYIDPKHLELEDRVVAINRVTKVVKGGRRLRFAALVVVGDKNG
                     HVGFGTGKAQEVPEAIRKAIEDAKKNLVEVPMVGSTIPHEVIGAFSGGRILMKPAVEG
                     SGVAAGGPVRAVLELAGVADITSKSLGSNTPINVVRATVEGLKQLKRAEEVAELRGKS
                     VEELIG"
     gene            54886..55065
                     /gene="rpmD"
                     /locus_tag="SCJ86_000471"
     CDS             54886..55065
                     /gene="rpmD"
                     /locus_tag="SCJ86_000471"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002356220.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L30"
                     /protein_id="HEG1168059.1"
                     /translation="MAELKVTLKRSIIGRPQNQRDTVKALGLTKINSSVVKPSNEAIK
                     GMINTVSHLVDVEEI"
     gene            55109..55549
                     /gene="rplO"
                     /locus_tag="SCJ86_000472"
     CDS             55109..55549
                     /gene="rplO"
                     /locus_tag="SCJ86_000472"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005592004.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L15"
                     /protein_id="HEG1168060.1"
                     /translation="MKLHELKPAEGSRQVRNRVGRGTSSGNGKTAGRGQKGQKARSGG
                     GVRLGFEGGQTPLFRRLPKRGFTNVNRKDYAVINLDVLNRFEDGAEVTPVALVEAGIV
                     KNEKAGIKVLANGELNKKLTVKAAKFSKAAQEAIEAAGGSIEVI"
     gene            55549..56844
                     /gene="secY"
                     /locus_tag="SCJ86_000473"
     CDS             55549..56844
                     /gene="secY"
                     /locus_tag="SCJ86_000473"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345495.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecY"
                     /protein_id="HEG1168061.1"
                     /translation="MFRLLKDAFKVKDIRSKILFTVFVLFVFRLGAHITVPGVNAASL
                     QNLSSLPFFNMLNLVSGSAMQNFSIFSMGVSPYITASIVIQLLQMDIVPRFVEWSKQG
                     EVGRKKLNQATRYLTLVLAFIQSVGITAGFNQYAQLGFVNNPNVATYVTIGLILTAGT
                     MFVTWLGEQITEKGIGNGVSMIIFAGIISRLPESIREIYEDYFINIDPSDIWKSAIFV
                     AILLVAVLLIVTFVTFFQQAERKIPIQYTKRVAGAPTSSYLPLKVNAAGVIPVIFASS
                     FIATPNAVLQALSGRFGSENWYKVMMEIFNYNTAPGATIYTILIVAFTFFYAFVQVNP
                     EKLAENLQKQGSYIPSVRPGKGTEEYVSRLLMRLSTVGAVFLGLVALLPIIAQMVWDL
                     PQSIGLGGTSLLIVIGVALETAKQLEGLMLKRKYVGFIE"
     gene            56906..57553
                     /locus_tag="SCJ86_000474"
     CDS             56906..57553
                     /locus_tag="SCJ86_000474"
                     /EC_number="2.7.4.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623850.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenylate kinase"
                     /protein_id="HEG1168062.1"
                     /translation="MNLILMGLPGAGKGTQAEKIIDAYGIPHISTGDMFRAAMQNETA
                     LGLEAKSYMDKGALVPDEVTNGIVKERLAEPDTEKGFLLDGFPRTLDQAKALDAMLKD
                     LNKKIDAVIDIHVGEEILVERLAGRFICSNCGATYHKIFNPTKVEDTCDRCGGHEFYQ
                     REDDKPETVKNRLAINIKNSEPILAYYKEQGLLNTIDGAREIDAVFADVQKIIEK"
     gene            57744..57962
                     /gene="infA"
                     /locus_tag="SCJ86_000475"
     CDS             57744..57962
                     /gene="infA"
                     /locus_tag="SCJ86_000475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003720929.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-1"
                     /protein_id="HEG1168063.1"
                     /translation="MAKEDMIEVEGTVVETLPNAMFKVELENGHQVLATVSGKIRMHY
                     IRILPGDKVTVELSPYDLNRGRITYRFK"
     gene            57995..58111
                     /gene="rpmJ"
                     /locus_tag="SCJ86_000476"
     CDS             57995..58111
                     /gene="rpmJ"
                     /locus_tag="SCJ86_000476"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003720928.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L36"
                     /protein_id="HEG1168064.1"
                     /translation="MKVRPSVKPMCEHCKVIRRKGRVMVICPANPKHKQRQG"
     gene            58129..58494
                     /gene="rpsM"
                     /locus_tag="SCJ86_000477"
     CDS             58129..58494
                     /gene="rpsM"
                     /locus_tag="SCJ86_000477"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008809894.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S13"
                     /protein_id="HEG1168065.1"
                     /translation="MARIAGVDIPRDKRVVVSLTYIYGIGNTTAKKILADAGVSEDVR
                     VRELTNEQTDAIRAEVDKLKVEGDLRREVNLNIKRLMEIGSYRGIRHRRGLPVRGQNT
                     KNNARTRKGPARAIAGKKK"
     gene            58522..58911
                     /gene="rpsK"
                     /locus_tag="SCJ86_000478"
     CDS             58522..58911
                     /gene="rpsK"
                     /locus_tag="SCJ86_000478"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016615824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S11"
                     /protein_id="HEG1168066.1"
                     /translation="MAAKKVNRKRRVKKNIEAGIAHIHSTFNNTIIMITDTHGNALAW
                     SSAGSLGFKGSKKSTPFAAQMAAETATKAAMEHGLKTVEVTVKGPGSGREAAIRSLQA
                     TGLEVTAIRDVTPVPHNGARPPKRRRV"
     gene            58991..59929
                     /locus_tag="SCJ86_000479"
     CDS             58991..59929
                     /locus_tag="SCJ86_000479"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010774253.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit alpha"
                     /protein_id="HEG1168067.1"
                     /translation="MIEFEKPRIAKIDEEKDYGKFIVEPLERGYGTTLGNSLRRILLS
                     SLPGAAITNIQIDGVLHEFSTIKGVREDVTQIILNIKGLALKMYGQEEKTLEIDITGP
                     ATVTAGDIIVDSDVEILNKDMYICSVSEGATFHARLTVKPGRGYVQADENKKEDMPIG
                     VLPVDSIYTPVRRVNYQVENTRVGHREDFDKLTMEIWTDGSIEPLEAMSLSAKIMTEH
                     LDIFVNLTDEAKNAEIMVEKEETQKEKMLEMTIEELDLSVRSYNCLKRAGINTVQELT
                     NKSEPEMIKVRNLGRKSLEEVKAKLHDLGLGLRKED"
     gene            59957..60337
                     /gene="rplQ"
                     /locus_tag="SCJ86_000480"
     CDS             59957..60337
                     /gene="rplQ"
                     /locus_tag="SCJ86_000480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013773093.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L17"
                     /protein_id="HEG1168068.1"
                     /translation="MSYRKLGRTSSQRKAMLRDLTTDLLINERIVTTEARAKEVRSTA
                     EKMITLGKRGDLHARRQAAAFVRNEVASVREENEDIVIESALQKLFNDIAPRYAERQG
                     GYTRILKTEPRRGDGAPMVVLELV"
     gene            60609..61448
                     /locus_tag="SCJ86_000481"
     CDS             60609..61448
                     /locus_tag="SCJ86_000481"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002356233.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="energy-coupling factor ABC transporter
                     ATP-binding protein"
                     /protein_id="HEG1168069.1"
                     /translation="MEPIIELEKINYKYQPDDLRPALKDVSFTIDKGEWIAIIGHNGS
                     GKSTLAKTINGLLLPESGIVKVGNQILDEENIWTIRQMVGMIFQNPDNQFVGSTVEDD
                     VAFGLENQGIPREEMLVRVKDALEKVRMAEFASREPARLSGGQKQRVAIAGVVALRPD
                     IIILDEATSMLDPEGREEVISTIKKIKEESQLTVISITHDIDEAANANRILVMRQGEL
                     VREGTPKEIFSAGPELIDLGLDLPFPEKLKSALKERGVDVPSEYMTEERMVDWLWTSV
                     LNK"
     gene            61424..62293
                     /locus_tag="SCJ86_000482"
     CDS             61424..62293
                     /locus_tag="SCJ86_000482"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177189.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="energy-coupling factor ABC transporter
                     ATP-binding protein"
                     /protein_id="HEG1168070.1"
                     /translation="MDIRFEQVDFTYQPNTPFEQRALFDINMTIKENSYTALVGHTGS
                     GKSTLLQHLNALVKPTSGTVHIGERDIQPDTDNKNLKPIRKKVGIVFQFPEAQLFEET
                     VAKDIAFGPKNFGVSEEEALVLAKETLEQVGLDESYLERSPFELSGGQMRRVAIAGVL
                     AMRPEVLVLDEPTAGLDPQGRKEMMEMFWRLHKEQQITIVLVTHLMDDVANFADYVYV
                     LEKGRIVNSGEPQEVFQNIEWLKEKQLGVPTATEFAEELMAKGMNFATLPLTAEELAD
                     AIVHYAGGNAHDE"
     gene            62283..63080
                     /locus_tag="SCJ86_000483"
     CDS             62283..63080
                     /locus_tag="SCJ86_000483"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853158.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="energy-coupling factor transporter transmembrane
                     protein EcfT"
                     /protein_id="HEG1168071.1"
                     /translation="MMNKLIFGRYMPGDSLIHRLDPRAKLLASFYFIGIIFLANNWET
                     YLLLAFFTLLSVFLSKVDLKFFIRGIRPLIWLILFTVALQVLFTSGGETYWSWGVFHI
                     TEFGVMNGLFIFCRFVLIIFMSTLLTLTTPPLELSDAIEYLLRPLKAVRFPVHEISLM
                     LSIALRFVPTLMDETEKIMNAQRARGVDFGEGNLVQKMKAVIPLLIPLFVSSFNRAED
                     LATAMEARGYKGGEGRTKYRVLHWHNRDTMVIIAFVLLTVLLIFLRG"
     gene            63283..64029
                     /gene="truA"
                     /locus_tag="SCJ86_000484"
     CDS             63283..64029
                     /gene="truA"
                     /locus_tag="SCJ86_000484"
                     /EC_number="5.4.99.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298563.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA pseudouridine(38-40) synthase TruA"
                     /protein_id="HEG1168072.1"
                     /translation="MVRYKAIIAYDGTNFNGFQKQPNGRTVQEEVEKTLQKMANGKEI
                     TVFGSGRTDAGVHAIGQVIHFDYPEERPLERMRFALDTQSPEDIAVRQVEIVSEDFHA
                     RYLVKEKTYQFRVDIGKPRSPFRRHYASYFPYPLDLEKIQRALPDLLGTHDFTSFCAS
                     GSSIEDKIRTIYEAKMEVNETKDELLFTFRGNGFLYKMIRILVGTLLKIGNGRLPEDS
                     IPDIIAKKDRNAAGPTAHPEGLYLYEVKYE"
     gene            complement(64362..67031)
                     /locus_tag="SCJ86_000485"
     CDS             complement(64362..67031)
                     /locus_tag="SCJ86_000485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330806.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfhO family protein"
                     /protein_id="HEG1168073.1"
                     /translation="MKKNLKSFFRENGRYAAASFLIPFLILALVYLSIGIYPGSSRSI
                     LASDAFSQFSNFHASFRNVLLGKQSIFYTWNASLGLNYLSLISYYLGGIFTPLVFFFP
                     NQMMPDALYVLTLLKVGCAGLAFWFLARTFKIPRWGHVALSVSYALISFITAHSEIIM
                     WLDAFVYLPLVILGIHRVMDLKRPKLLFISYLLLFLSSFYMGFMIGVFSFLYFIARLL
                     TNWSEYKKTIVPYGITSLLAGGASMIIVLPAVLDLRSNGEALSQITTLKTEATSYLDL
                     IMKNMIGVYDTTKYGSIPFVYAGLLPLVLCLFYFASRKVPLKNKFLYGSLFAILIASF
                     YLVPLNLFWHGMHAPNMFLFRYAYLFSFLVLLLAGKGWEKIEQDDGGLLLGIILVVAA
                     LFAVAWGIKPEGSYTYVTMTSFVLTVVFLGIYAFAVGFYQHKKLSFNHLSILLLLIMS
                     AEALVNTNSMIHGILDDWNYASRSLYTDPYPDLKALVNETKKDSDSFYRLENLDPVSA
                     NDSINYGYSGISLFSSIRNRNSSSYLDKLGFRSRGTNLNIRYNNNTLLMDGFTGIKYN
                     ISKNESTFSKYGFRQAGQSGDYTLYQNVNALPLAFTAPLSVNDVEQPVTDNLTSQTNL
                     INTLSGLNEQYFTFYTPTLKNQLNTTITKTTTGVTYSETADNQPKELTWEVHVPANTQ
                     AYLSLFPTNFAQLESSSATITVNGTSRKTQINISGQYYDLGYYPQETTVTFTASFYGT
                     KEISFMEPKVVGLDVVAYQAAMNQIKEKGVEMEATGRKASGTVNTAEDSMLVTTIPYD
                     KGWKAKIDGKPVKVSNFKDAFLMIKVPAGKHTVTFSYLPEGFTIGAILFVLCIALFIL
                     YVWYLDRNRPFYGTIDPAIEEQPRNTHRRRRRK"
     gene            complement(67269..67457)
                     /locus_tag="SCJ86_000486"
     CDS             complement(67269..67457)
                     /locus_tag="SCJ86_000486"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007207596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L28"
                     /protein_id="HEG1168074.1"
                     /translation="MAKVCYFTGRKTKSGNNRSHAMNSTKRTVKPNLQKVRVMVDGKP
                     KKVWVSTRALKSGKVERV"
     gene            67727..68089
                     /locus_tag="SCJ86_000487"
     CDS             67727..68089
                     /locus_tag="SCJ86_000487"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002354933.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Asp23/Gls24 family envelope stress response
                     protein"
                     /protein_id="HEG1168075.1"
                     /translation="MAVKIQTSAGTIEITNDVIATVVGGAATDVFGVVGMASKNQIKD
                     NITEILRKENYSRGVVVRQEDNGIAVDVYTIISYGTKISEVSRNVQEKVKYNLEALLG
                     VTANSVNVFVQGVRVLPD"
     gene            68141..69823
                     /locus_tag="SCJ86_000488"
     CDS             68141..69823
                     /locus_tag="SCJ86_000488"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629817.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DAK2 domain-containing protein"
                     /protein_id="HEG1168076.1"
                     /translation="MSVTEISASQFQEMVQAGANRLQVNAEYVNSLNVFPVPDGDTGT
                     NMNLSMTSGAKAVVDSTSEKVGELAAMLSKGLLMGARGNSGVILSQLFRGFSKQIPDV
                     VSLNASDLAAAFTHGVETAYKAVMKPVEGTILTVAREAAKAGEKKAKSTDDVVEVMTA
                     VVKGAKRALAKTPDLLPVLKEVGVVDSGGQGLLFVYEGFLSALNGEFQADETYEPSPA
                     EMDDMVNAEHHRSVQGQMATEDIHFGYCTEIMVKIGEGPTVDSTFDYEEFRNYLDGIG
                     DSLLVVNDDEIIKVHVHTENPGEVLNYGQKFGSLVKIKVDNMRLQHETILEHDEEVNE
                     FAKKEIPRIPYGIVAIAAGKGVQELFESLGANYVISGGQTMNPSTEDIVKAIQEVNAD
                     KVIVLPNNKNIFMAADQAAEVADIPVAVVPSRTISQGLTAMLSFNDQASLEENKETMT
                     EMLSSVVSGQVTHAIRDTSIDGVEIKEGDFLGMIDGKILISNPDITATALSTLEQMIN
                     EDTEIVTILAGDEGTVEQANEFAEKLTEKHPDLEIEIHQGDQPVYPYLFSAE"
     gene            69940..71976
                     /gene="recG"
                     /locus_tag="SCJ86_000489"
     CDS             69940..71976
                     /gene="recG"
                     /locus_tag="SCJ86_000489"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent DNA helicase RecG"
                     /protein_id="HEG1168077.1"
                     /translation="MRSLDDPITMLAGVGEKRAASLASLGIQTIEDLLTYYPFRYEDI
                     QERNLNEIQDQEKVTLKGIVVSPPVMNRFGYKKSRLQFRMMQEHDVFNVSFFNQPYLK
                     EKVILSEEIAVYGKWDAKRKALNGMKILGSQTMDDFSPIYHVNKSIRQTTLVDLIKKG
                     FQEYGTLIEENLPNNLVEKYRLLDRASAVRSMHFPKDHEENHQAKRRVVFEEFFLFQM
                     RLQGLKKAEKAETNGLEILYDVQKLKQFTQKLPFELTNAQKRVTNEICRDLRSPQHMQ
                     RLLQGDVGSGKTVVAAIALYATVTAGFQGALMVPTEILAQQHMESLQQLFDPNEVTIA
                     LITGSTKAKERRELLEKLEQGEINVVIGTHALIQDGVEFQNLGLVITDEQHRFGVNQR
                     KVLREKGWRPDVLFMTATPIPRTLAITAYGEMDVSIIDELPAGRIPIETRWVRTPQLD
                     SVLDWTKKELARGHQMYVICPLIEESEALDVKNAVEIYEKLRELFAPKYEVGLLHGKM
                     KNQEKEVIMEAFKDNQMQILVSTTVIEVGVNVPNATVMLIMDADRFGLAQLHQLRGRV
                     GRGSDASYCILVANPKNELGVERMKIMTETTNGFVLSEKDLELRGPGEVFGFRQSGLP
                     QFAIADLVTDGNVLEVAREEAAALWQIKDWQMLPEYQPLAQSLQIENQDNRYFD"
     gene            72025..73026
                     /gene="plsX"
                     /locus_tag="SCJ86_000490"
     CDS             72025..73026
                     /gene="plsX"
                     /locus_tag="SCJ86_000490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate acyltransferase PlsX"
                     /protein_id="HEG1168078.1"
                     /translation="MKIAVDAMGGDHAPQAIVEGVALAQQDFPEVEFLLYGKEAEIKK
                     YLTNEKNITIIHTDEKINSDDEPVKAIRRKKTASMVLAAQAVKNGEADAVFSAGNTGA
                     LLAAGLFIVGRIKNIERPGLMSTLPVVGKEGAGFDMLDLGANAENKPEHLLQYGILGS
                     FYARKVRGIGRPRVALLNNGTEATKGSEVTKKAYELLQNETSLNFIGNVEARELLNGV
                     ADVVVTDGFTGNAVLKSIEGTAMNMMNLLKSAILNEGIKGKMGAMLLKDGLRSLKAEM
                     DYSKHGGAVLFGLKAPVIKTHGATGPDAVRYTIRQIHTMLETDVVGQLVEQFEKSEE"
     gene            73148..73390
                     /gene="acpP"
                     /locus_tag="SCJ86_000491"
     CDS             73148..73390
                     /gene="acpP"
                     /locus_tag="SCJ86_000491"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294295.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl carrier protein"
                     /protein_id="HEG1168079.1"
                     /translation="MTREEVFNKVAKIISNHFEIDTDKVTDELNIKDDLKADSISIME
                     FVLELEDEFGTEISDEDAEQIETVGGAVDYISSHLK"
BASE COUNT        24123 a        13094 c        15406 g        20780 t
ORIGIN      
        1 atatagtagg aatcggacgc tcagtacgga gcgtctattt ttttacccaa ttttgaaagg
       61 aagtgaactt atgaaaataa aaaatcaaga atcaaaaggt cgctctcccc cctttaaaac
      121 catcacacaa ataagattca ttcaaacata aaaacagaaa ggataggtaa aaatatggaa
      181 cttaaatatg tcattcccaa tatggaaaaa acatttggac aattagaata tgctggtgaa
      241 gggaacattg aacaacgtcg aataaatggc agaccaacca ttctctctcg tagttacaat
      301 ttatactcaa cgattcaacg agcagatgat attgttgtca ttcttccctc cgaagctggc
      361 gagaaacatt ttgaatccga cgaaaaagtc aaactcatca acccacatat taccgcagaa
      421 ggttataaaa ttggcaatcg aggatttacc aattacatta tgcacgcaga cgatatggtg
      481 aaagcataag aaaggaaagg tgaaaaaata tgagattagc agaaggtatt gtgattgaaa
      541 aagaccagac atttggcaca ttgaaatttt ccgccttgcg tcgtgaggtt cgtatccaga
      601 atgaagatgg tacggtttcc gaagaaatta aagaacgtac ctatgactta aaatcaaaag
      661 gacaaggacg catgatacaa gtcagtatcc ccgcttctgt tcctttgaaa gaatttgact
      721 acaacgcaga agtggaatta atcaatcctg tggcagatac cgtagcgacg gctacttttc
      781 gaggggcaga cgttgattgg tacgtgaaag cagacgatct tgtattaaaa ggacagacaa
      841 aacctcatca gaaatataaa aaagaagcgt ccgaaagtga aaaataggtg ttgcttatga
      901 caaacacacc attgattttg aaagaaattc cgaaggatga agccatttct tttattcggc
      961 aataccatta ttcaaagata ctccctagat tgtgtaaata ctttctaggg attttttcag
     1021 aagaaaaatt attaggcgtt gtggaattag gctggggtac gcaaccccta caaaccatac
     1081 gcaaactctt tcccgacagt tcccttcaaa ccacagatta tttagaaatt ggaaagatgt
     1141 gttttcttcc agaaatgaat caaaccaatt attttggcag tcaagctctt tcagctctaa
     1201 ttaagtggtt gaaagaacat acggactgtc attttttata tacactggca gatggaattg
     1261 agggaaaatg tggctatgtc tatcaagcgt ccaacttctt ttactgcggg tatttcaaga
     1321 caagtgtcta tcgtgataag cagtcatggg aaaagataca ccctcgaagt gctcgccttt
     1381 tattagagga aaatgctcgc tttgaacagg tagagaaaaa gcattggctt tcccaggcat
     1441 tttgcgaata caaagggatt gagaaaatca atggacgcat gtttcgctat ctctatccgt
     1501 tgacaaaaga agcgaaaaaa ctgttaggac acactctcta tcgacgtcat tattatccga
     1561 aagaaaaaaa tttacgcttt gaaaagcgaa tcgcttatca gaaatatgaa gccatttctc
     1621 aaccaacttt tgataaacag gctcgtatct acaatacaca attattttga taggtggaaa
     1681 ggaggatttt tatgccattg cttcatgaca gaggagagcg aatcaaagcc aacgataagc
     1741 acttggtcta tcattttgta ctaatttcat tgctggtagt ttttctagtc gtcctactcc
     1801 cttttcattg gagagcgtta caatcggtga actggcaagc agttcagtta gatacatttt
     1861 tctatcaact tcatacgcca tacttttgga gcagtattct cactgctctt tttgtatgcg
     1921 gacttatcag ctggctttat tatcactatc gtatagattc tataaaacag ttagagcacc
     1981 gtcaaaagtt agctcgtatg attttagaaa atcattggta tgaagccgaa gaaactcaat
     2041 cagagggatt ctttaaagat ttacccagca agaaaaccaa acaacgaatc cgccattttc
     2101 ctaaaatata ttatcgaatg aaagatggac taatccatat tcatgtagaa atcaccttag
     2161 ggaaatatca agatcagctt ttgcacctag aaaacaaact agaaacagga ttgtattgtg
     2221 aattagtgga aaaaacgttg aaagattctt atgtagagta tgtcctactt tatgatacta
     2281 ttggcaatcg aattggcatt gaagacgtgg tagctcaaga tggacgtgtc aaattgatgg
     2341 aatcggtcta ttgggagttc gattctctcc ctcacatgtt aattgctggg ggcactggta
     2401 gtggaaaatc ctattttatt ctcacattga ttgaatctct cctgcataca aatgcaaagc
     2461 tttatattct tgaccctaaa aatgctgatt tggcagactt aggggcggtc atgaataatg
     2521 tctattaccg taaagaagat atgttggcat gtatcaatca attttacgaa gatatgattg
     2581 atcgcagtga aaccatgaaa caacacccta actataaaac aggagaaaat tatgcttatc
     2641 ttggtttgtc tgccaatttt cttatttttg atgaatatgt ggcaatgatg gatatgctag
     2701 gtcgtgaaag taccaccgtt attcataaac tcaaacaaat tgtaatgtta ggacgacaag
     2761 caggcttctt tttaattctt gcctgtcaac gtccagacgc aaaatatttt agtgatggga
     2821 ttcgggacaa ctttaacttc cgtgtggctt tgggtcgtat gtctgaaatg ggctttggta
     2881 tgatgttcgg aagcgataca caaaaacaat ttttcttaaa acccatcaag ggaagaggtt
     2941 acgtggatac aggaaaaagt gtgattagtg agttttatac accccttgta ccaaaaaggt
     3001 acgatttctt aggagaaata ggaaaagtca ttcaaaaaaa gcaatctgag cctgtaccgc
     3061 acgaagtgaa aggtacaggt tcagacttgc tttgattggt gtggcgttag ccacgccaat
     3121 cctttagccc cctctatcta acaggggggc acaaatacaa ctgaaaacag gcaattctca
     3181 tcaaccaatg ttgatggtac aaaggattgc ggaaaatatg aaaggtgtaa actcaagggt
     3241 catagtttac atctttttta gattggaggt ttgcgaatgg aaaacgaaaa aatttggatt
     3301 aaggaattga aagaaaagcg actggcttat ggcgtttctc aaaataaact ggcggttgct
     3361 tctcatatta ccagacctta cctatcggat attgaaacag gcaaggcagt ccctactcaa
     3421 caggtgaaag aagatttact gaacgctttg gaacgcttca atcccgataa cccattggag
     3481 atgttattcg actatgtacg gattcgattc cctaccaatg atgtggctca aattatcggt
     3541 gaggttttac gtttgaatat ggattatatg ctacacgaag actttggcta ttattcttat
     3601 cccgaacact atcgtttcgg tgatattgtt gtcctagtct ctcatgatgt aagtaaaggt
     3661 gttttattag aactgaaagg aaaaggctgt cggcaatttg aaaactttct attggctcaa
     3721 catcggagct ggtatgattt ctttcaagat tgcatggaac ataaaggtat tttcaaacgc
     3781 ttggatttag ctatcaacga taaaacaggg attttgaata ttccagattt ggcaaagaaa
     3841 tgtaaacagg aagaatgcat ttccgtcttt cgaagcttta aaaattatcg cagtggtgaa
     3901 ttggttcacc gtgatgaaaa accatacatg ggaaacacac tttatattgg ctcattaaaa
     3961 agtgaagtct atttctgtat atatgagaaa gattatgagc aatacgtgaa aaatgatgtt
     4021 cccttagaag acgcagaagt aaagaatcgg tttgaaattc ggttaaaaaa tgaccgagcc
     4081 acccaagcca tgaaagattt attggcacat caacaggcgg aaaaaacagc ctttgaaatt
     4141 atcaatcgct atatccgctt tgcagataaa gacgacacta aacgtcggag tgattggaaa
     4201 actaatgaac gttgggaatg gtttatcggt aaaaatcgtg gagcattacg cctaaccaca
     4261 cagccagagc cttattcttt tgaacgaaca ttgaactggt tacatcatca agtcgcccct
     4321 acattaaaaa tcgcaagcat tttagatgtt ctcaacggta caaccattat ctccaccatg
     4381 attcaggaag ccaaactgac agaaaaacat gagaaattaa ttgaacaaca acatctagcc
     4441 atggaggatt tgattacatg agccttttaa caaaagaatt aaagaagtta gggtttcagt
     4501 gtggcattga gtttcaagcg tatatacaaa atacagggaa atatacgtct ctcattattg
     4561 agggaaaacg gcaagctggt gataccatct atacctatga tttttacaag gtgagatttt
     4621 ataataatta taccaatcga gtaaccgtct atggagagca tttaacccct tttcaattat
     4681 taaggcgtgt aaaaagctat atctattatc gagaaaaata tcttaaggaa aggaggacaa
     4741 tcacatgaat tttgggcaga atttgtacaa ctggttcttg gataatgcac agtcattggt
     4801 attgttagcg attgttgtca ttggactgtt tttaggtttt aaacgagaat tttcaaaact
     4861 cattggcttc ttgattattg ctctaatcgc cgtcggactt gtgttcaatg cagctggcgt
     4921 gaaagatgtg ttattaaact tatttaaccg aatcattggt gcttaacaga tatttttcat
     4981 gataaaatag gaagtatata ttttgttatg aaaggattta tgaatatgtt tacaaatgat
     5041 caatttaaat ctcttttaat taaaacatta gaaagaaaga gctcttcaaa ttattatgaa
     5101 ggaggtaacg agattgtttc taaaatgaaa atatcagagg taactttaga tgagactgac
     5161 gattttactt attataaagg atataaaaag ggctggaata cgctttgcac atatcttaaa
     5221 ataagagttc cttttgatga tttagacttt tttgaaagtc ataaagattt gattacccag
     5281 acagcaagct ccatatatga taaacaaggt gataatgtat tagtggatac tattttagta
     5341 ccacttccag agaattacga agtaattaac tttagtcagt tgaaaattag tgatgtggtt
     5401 tcacaagcta ttgaagacgc tgagtctttt atgagtaatg gtgaatatca acgtgccttt
     5461 gacagagttc acactgcttt tcatggatat ttaattgaaa tactaaaaaa atatgaaatt
     5521 acagttccta gagacgaaaa tctatctaaa ttatatagta ggatacaaca attaattgaa
     5581 aaagaaattc aacctactga acttgctgat atagtgaaga caacgataag gtcatcaaat
     5641 ggaatgatta gctctcttaa tgaagcaaga aataggcatt cacttgcaca ccctaataca
     5701 aatattattg gaaagagaga agcaaaacta ataataggta tttcaagtac agttacagat
     5761 tatattagcg gataccttga taagtagcgt cattaactga ttgctacttt ttttatgact
     5821 atttttataa aaaggatgtg ataagaattg gaaatgaaag tctatgtggc aaatttagga
     5881 cggtataatg aaggtgaatt agtcggagca tggtttactc caccaattga tgaggaagaa
     5941 atggcagaaa gaatcggttt aaatgaggac tacgaagaat atgcaataca tgattttgag
     6001 cttccttttg atgtggacga atacacgccg attagtgaaa tcaaccgtct atgtgaagcc
     6061 attcaagaaa tcgaaggtac acctatttat aatgaactca aagaaattca aggcatgtgg
     6121 tttagtagct tggaagaatt actggaaaac aaagaggata ttcattgtta tagtgattgt
     6181 gattcgatgg aagatgtcgc ccgttattac gtggaagaaa caggacagct tggtgaagtc
     6241 ccttccaatt tacaaaatta tattgactac caatctttgg gacgtgatat ggaaatagag
     6301 ggtaattacc tagttacctc tcatggggta tttgaatatt gtcagtaagt ttaacaatcc
     6361 gtgttataat taaattaata aatagcacgg agaggtataa attatggtaa atgtatttga
     6421 aaatcttaaa ctattaaaac tagacatgaa agataaaggc tgggtgatag attcctttta
     6481 ctttcgttac aaacagcaaa attatattgt acttgttaag ctatttgaaa aagaggaaaa
     6541 agttccagaa tatgctctgt taaaactaga atttttaaag gaaaacgatt tttcagatat
     6601 gctagctgtc tatgctaatt cggtaaaatt atatacagac acgaaaacaa taagagaata
     6661 ttttggtatc gaatacagta gtaacttggg agatgtttta tttcaattta gccaaactct
     6721 tgctcgattt attccgacag aagttagtga gaagaaaaat gaagaccaaa aagaagccat
     6781 gtgtttctca ttaagtcaaa gtgattcaga agatcccaga aaaaaatatt gtttttcagt
     6841 tagaagaaat cctttaaagg gaaatggtga gctgggaaaa cgaagtcctt ttaatgacaa
     6901 taaaacgaga ttgtatagac ccagcttata tgaacgatta gggtcagata ccaatttaag
     6961 tttcagatat tcaatgaatc cagatgatga agaaactgat gaaggtatta ttgcaaaatg
     7021 gacaaaaaat aaaattgaat agttaaagca ttgaaagagc aatcacctat tttgattgtt
     7081 ctttttctat ttttacggaa aggacgtgaa gacatgaaaa aaataagaag ttacaccagt
     7141 atttggtcag tagaaaaagt gatttatgcg attaatgatt tacaactccc tttcccaatt
     7201 acctttacgc aaatgacatg gtttatcgtt tctttgtttg tcgtcatact ctttggcagt
     7261 ttaccaccac tttctctgat tgacggagca ctactcaaat acttagggat tcccattgtt
     7321 ctcactgggt tcatgtgcca aaagaccttt gacggaaaga aaccctatgg atttctaaag
     7381 tctatcatta cctatgctct acgccctaaa gtaacctacg ctggcaaaca aatacgatta
     7441 aaaaaagaaa caatgaatga atcgattacg attgtaagga gtgaacaact agatggcata
     7501 tccgattaaa tatatggaag ataatctcgt atttaatcat gatggcgaag tattcgctta
     7561 ttatgaatta attccctata attacagttt tttaagttcc gatgaaaaaa ttcaagttca
     7621 tgataatttc tgtcaattga ttgcccaaaa tcgtgatggc aagattcatg ctctgcaact
     7681 agcaaccgaa tcaagtattc gagccacaca ggaacaatcg aaaaaagaaa tcactggacg
     7741 attaaaagag atagcttacg aaaaaataga tgaacagacc gaagcgttaa tttctatgat
     7801 tggtgacaat caagtagact atcgcttttt tattggtttt aaactctacc tcaatgaaca
     7861 ggaagtatcc gtaaaaaatg ttgggaaaga aattcaaaaa gcatttgcgg aattctttca
     7921 tggtgtgaat cacaaattaa tgggcgattt tgtttctatg cctatggcgg aaatcgaacg
     7981 cttttctaag atggaacagt tgttagaaag taaattatcc agacgctttc agattcgtaa
     8041 attggagaaa gatgattttg gttatattat tgaacatctt tacgggcaaa caggtgtccc
     8101 ttttgatgac tacgactacc atttaccgaa acgatacgaa gataaaaaag tgttggtaaa
     8161 gaaatacgat attctcaaac ccactcgctg tttaatcgaa gaaaatcaac gctatttacg
     8221 gattgaacag gaagatacca ctacttatgt ggcgtatttt acaattaata gtattgttgg
     8281 tgaattggat ttccctaata gtgaaatttt ttattaccag caacagcagt ttactttccc
     8341 cgttgatacc agtatgaatg ttgaaattgt cgccaataaa aaagccttat ctaccgttcg
     8401 caataaaaag aaagaactga aagatttaga tagccacgca tgggaaagcg acaacgaaac
     8461 cagtactaat gtggtggacg ctttagaatc tgtcgatgaa ttagaaagta atttagacca
     8521 aaccaaagaa tctatgtaca aactaagcta tgtggtacgg gtatcagcaa agaatttgga
     8581 cgagctgaaa caacgttgtg atgaagtaaa agatttttat gatgatctgt ctatcaaatt
     8641 agtacgacct tttggcgata tgctaggctt gcataatgaa tttattccct caagcaagcg
     8701 atatatcaat gactatattc aatatgtcac gtcggatttt ttggctggtc ttggtttcgg
     8761 tgctacacaa atgattggtg aaaattctgg tatttacgtc ggttacaatc tcgatacagg
     8821 cagaaatatt tatctccaac ctagccttgc aagtcaagga attaaaggct ctgtaacaaa
     8881 tgcattagcg tccgctttta ttggttcatt aggtggcgga aaatcattct ctaacaatat
     8941 gctggtctat tattcagtac tctttggcgg acaggcaacc attgttgacc caaaagctga
     9001 acgtggtgaa tggaaagaaa ccttacctga tattgcccat gaaatcaata ttgttaatct
     9061 cacgagtgag aacgacaata aagggttact tgacccttat gtgattttaa aactcccgaa
     9121 agattcagaa agccttgcca ttgatattct cacgttctta acaggtattt ccagccgaga
     9181 tagcgagaag tttcctgtcc tgcgtaaagc tattcggagt gtaactcaaa gcaaacaacg
     9241 agggttgtta ttggtgattg atgaattgcg aaaagaagat acgcccatca gcaatagtat
     9301 tgctgaccat atcgaaagct ttgtcgatta tgattttgcc catctgttat tcagtgatgg
     9361 cagtgtcacc caatccatta gcttggaaaa acaactcaat attattcaag tggcagattt
     9421 ggtcttaccc gattccaata gctcttttga agaatacact accatggaat tattaagtgt
     9481 ggcaatgctg attgtcatta gtacctttgc tttagacttt atccactctg accgttccat
     9541 ttttaaaatt gttgaccttg atgaggcttg gagtttttta caagtggcac aaggaaaagc
     9601 tctatctatg cgattggttc gagcaggacg tgccatgaat gcaggggtct atttcgttac
     9661 tcaaaacgcg gacgatttac tagatgaaaa gatgaagaat aatattggct tgaaatttgc
     9721 ctttcgttcc actgacctag tggaaatcaa aaagacctta gaattttttg gtgtggacaa
     9781 ggaagatgaa aacaatcaga aacgcttacg tgaattagaa aatggacaat gtttagtttc
     9841 agacatctat ggtcgtgtag gggttatgca gtttcacccg atttttgaag agttgcttca
     9901 tgcctttgat accagaccgc ctgtgcgaaa tgaggtggaa gaatgacaca aaaacaaaag
     9961 aaatttcttc actatagttg gataacgctc ctttgtgtgg gcgttatttt acttttatta
    10021 ggcacgctgg gcaatgcagt acaagcgaca ggcttagttg atgaaaccat tgatacaagc
    10081 aatgaatact ccaagtatgg tttgaatcaa tatcagcttg attattatgt ggacaatagc
    10141 tggggctggc ttccatggaa ttggagtgat ggcataggac aatcggtgat gtatggattg
    10201 tatgccatta ctaattttat ttggacaatt tctctttatc tatcgaacgc cacaggttat
    10261 ttggtgaaag aagcctatgc cttagatttc attagtcaaa catcccaagc aattggcgaa
    10321 aacatgcaga cgattgcagg cattacaccg aatggctttt ccagttcagg gttttatgtc
    10381 ggtttcctcc ttctttttat tctcattgtt ggaatctatg ttgcctatac aggattggtt
    10441 aagcgagaaa cgacgaaagt gatacgagct gttttaaact ttgtcgtggt gtttattcta
    10501 tcggcttcct ttattgcgta tgcacccgat tatattgcga aaatcaatga tttttccaaa
    10561 gatgtcagta ccgccagtct gaatattggg acaaaaattg ttcttcctaa cacggatagt
    10621 caagggaaag acagtgtgga catgatacgt gacagcttat tctctgttca agtaaagcaa
    10681 ccatggctat tgcttcaata tggctccact gatgtagaag cgttagggga agaacgtgtg
    10741 gaaagtttat tgtctaccag tccagataca aacaatggtg aagatagaga aaatgtagtg
    10801 attgaagaaa ttgaagatca tgacaatacc tatttaaccc taccgaagac gatttccaga
    10861 ttaggaacag tcttcttttt gtttgtattc aatattggta tctcgatttt tgttttcctt
    10921 ctaacaggaa tcatgatttt ttcgcaagtg ctatttatta ttttcgctat gtttttgcca
    10981 atcagtttcc tattaagtat gttgccgagt tttgatggca tgggcaggca tgccattacc
    11041 aagttattta atgtgattat gacaagggct ggtattacct tgattattac cgttgccttt
    11101 agtatttcca ctatgcttta ttcgctgtca gcaagttctc cctttttcat gattatgttt
    11161 ttacaaattg tgacctttgc agggatttat tttaaactgg gcgatttaat gagcctattc
    11221 actttacaaa gcaacgactc tcaaaacatg ggcagacaag tgtttcgcag acctcgtcaa
    11281 atgatgaatc gacaaaccag aagatttcaa cgcagtatga gtaagatttt tcgtggaaaa
    11341 gcagggtcaa cgaagcaaaa gacaatgaca acaaagcaga atcaaaccaa taaagctaac
    11401 catactcgtc aaaatgaacg gactacacca aagaaagaaa cagcactttc caatgtgaaa
    11461 aagcaacaag gcggtatcgg gcaacgatta ggtgaaaaga cagcccatgt attggacacg
    11521 aaaaatcgtc tagtcaataa aagaaagcag gcaaaacaac agatacaggc gacaccaacc
    11581 catgtcaaat atgcccttca tcaaggaaaa gagaatgtca ctcataatgt aacagacttt
    11641 aaacaatcta tggcagatac caaagctcaa cgcaaacaac agcgtatgaa ccagcaggag
    11701 caaaaacgaa agaacattgc tcaaaagcgt ttggagctgg ataaagcaaa agaaaaaaga
    11761 cgaaaagaaa acccatcatc tcctgttcag aataaccagc agcatgttcg caagcgtcag
    11821 caaaccgtca cgcctgtttt gtcacaaaac actaaacgtg aaaatcatca gcgaccattg
    11881 aaaaaagagc aaaaacaaaa gacaccaccg aagcaattca atcagaggaa gctaaataaa
    11941 agaacggata agcgaggaca acaaacatga agctaaaaat aacagtgatt tgtagtgtgg
    12001 tcttctctct cttctttttt ctcttattat tcctagtcat tttctttgca gatgatacgg
    12061 acagtggcga aaacaacaag gattcttcta ttccacaagg cggtgtgacc gtttcaccag
    12121 aagtgttggc acaccgtccg ttgattgaaa aatatggtaa ggaatatggg attgaagact
    12181 atgtttctta tatcttagcc attatgcaag tggaatcggg cggtacagca gaagatgtta
    12241 tgcaaagttc cgaatctttg ggtttaccgc ccaacagttt gagtaccgaa gagtccatca
    12301 aacaaggggt taaatatttc agtgaattat taaccagtgc agaacaacaa ggagtggata
    12361 tagattctgt tatccaatct tataattatg gcggtggctt tttaaattat gtgaggagtc
    12421 atgggaaaaa atatacctat gaattagccg aacagttttc taaagagaag tcgggtggtc
    12481 aaaaagcaga ctatcccaat cctatagcca tacctgtgaa tggtggttgg cgatataact
    12541 atggaaatca gttttatgtt cagttggttt cacaatacct aaccgacact tccccaacag
    12601 aatttgatga tgaaaccgtg caagtcatta tggacgaagc cttgaaatac gaagggtttc
    12661 cctatgtctt tggaggggcg tcgcctacca cctcttttga ttgtagtggc ttaatacaat
    12721 gggtgtatga caaagctggc atttctctcc ctcgtgtcgc ccaagatcag tatgacgcta
    12781 cgcaagaaat ctctatggaa gaagcccaag caggcgacct tatcttcttt cattcaacct
    12841 ataatgcagg cacatatgtg actcatgtgg cgatatattt agagggaaat cgcttttatc
    12901 atgcaggcga cccaatcggt tatggcgact taagcagtcg ttattggcaa gaccatctga
    12961 ttggggctag acgtgttatt cataactaag aaaggaatga atcaaatgat tataaaaatc
    13021 gagcgaaaag aaaagaagga gaaaccaaag aaatctaaaa aaccgagcaa gcaaagaaaa
    13081 gttccgatga ttaaagttgg cacgcataag aaattaacct ttgttttatg ggttttattg
    13141 attggtagtg tcggatttgg aatctataaa aattttactg ccattgacac tcatactgtc
    13201 cgtgaaacag aaatcattaa acagcagatt gtcgatacca accaagtaga aagttttgtg
    13261 aaatcctttg cgaaagacta cttttcgtgg caacaatccc aagaagccat tgataaacga
    13321 aatgaaaaat tgacccatta tttgacggaa gaattgcagg tattaaatga agaaatgatt
    13381 cgtaaggata ttcctactag ttctagtgtg aacgatatac aggtgtggca ggtttctcaa
    13441 gtgaatgaga acacctttga ggtactgttt tctgttgaac aagttattac cgaagacaaa
    13501 gacaaggaaa ctatatcgtc tagctttcat gtggttgtcc atatagatga atcagataat
    13561 atggttatta tcaaaaatcc gacaatgagt aagaaacctc aaaaatcgga ctaccaaccc
    13621 aaacagctag aaagtgacca tacagtagat acagaaacga tggacgaaat cactagcttt
    13681 ttagaaacgt tcttccagct ctatcctacc gccacagaaa aagaattgac ttactatgta
    13741 agtaatcatg tgttacctat gattaacaag gaatatgttt tcgaggaatt ggtgaatcct
    13801 atctttacca gaaaagataa ccaagtaatc gtgaatgtcg ctgtgaaata tttagatcaa
    13861 gaaacaaagg caacccaaat ctcgcaattt gagttgatat tagaaaaaca agataattgg
    13921 aagattgtaa aataacaaat attggtacat gattacagat actttgtaat catgtactct
    13981 ttttgataaa aaattggaga ttcctttaca aatatgctct tacgtgctat tatttaagtg
    14041 actatttaaa aggagttaat aaatatgcgg caaggtattc ttaaataaac tgtcaatttg
    14101 atagcgggaa caaataatta gatgtccttt tttaggaggg cttagttttt tgtacccagt
    14161 ttaagaatac ctttatcatg tgattctaaa gtatccagag aatatctgta tgctttgtat
    14221 acctatggtt atgcataaaa atcccagtga taaaagtatt tatcactggg atttttatgc
    14281 ccttttgggt ttttgaatgg aggaaaatca catgaaaatt attaatattg gagttttagc
    14341 tcatgttgat gcaggaaaaa ctaccttaac agaaagctta ttatataaca gtggagcgat
    14401 tacagaatta ggaagcgtgg acaaaggtac aacgaggacg gataatacgc ttttagaacg
    14461 tcagagagga attacaattc agacaggaat aacctctttt cagtgggaaa atacgaaggt
    14521 gaacatcata gacacgccag gacatatgga tttcttagca gaagtatatc gttcattatc
    14581 agttttagat ggggcaattc tactgatttc tgcaaaagat ggcgtacaag cacaaactcg
    14641 tatattattt catgcactta ggaaaatggg gattcccaca atctttttta tcaataagat
    14701 tgaccaaaat ggaattgatt tatcaacggt ttatcaggat attaaagaga aactttctgc
    14761 cgaaattgta atcaaacaga aggtagaact gtatcctaat atgtgtgtga cgaactttac
    14821 cgaatctgaa caatgggata cggtaataga gggaaacgat gaccttttag agaaatatat
    14881 gtccggtaaa tcattagaag cattggaact cgaacaagag gaaagcataa gatttcagaa
    14941 ttgttctctg ttccctcttt atcatggaag tgcaaaaagt aatataggga ttgataacct
    15001 tatagaagtt attactaata aattttattc atcaacacat cgaggtccgt ctgaactttg
    15061 cggaaatgtt ttcaaaattg aatatacaaa aaaaagacaa cgtcttgcat attaagtagt
    15121 tcagttttgg agtacaaaaa gtgaaaatgt aaacctaaat gagtatcaaa ttgcaactga
    15181 atttgatact cgttttttat tgttaaatca acgtttgttg ttaatttaca ccacaaaatg
    15241 taagttaaaa tgtatataaa aatgcgaacg tgaaataatt attgtaggaa tgcaaattgg
    15301 tgtgttaaaa tggttacaat ttactaagca aaaaggggtg cattttccta tgcaatttct
    15361 tgagccaaaa aataagaacg ctaagtctgt ggattgggaa atatcagagc aagtaagggt
    15421 gatagttaag caatacgctg aatatgcaga gcgtactgaa agcgaagcag tggatgaatt
    15481 cttattgaac attttagacg ataagaagtt tattgaatgg attgccaaca agcgaagtaa
    15541 taagcggatt gttgaaaaaa tgggtatcaa agatagagtg ggttgataga caatgaccaa
    15601 gcttaaaaga ttagccacga aggaagatga aattgtagat gttaaaattc cgatttcaaa
    15661 cgaagaatta aaagataggg ctaaacaata tgatttatta actccaaaac gatttgcaac
    15721 aaggtataac aaaatgttgt tcttgccaac gtcttttaaa tggaacggtt ctgaatatcc
    15781 cattcaatat aattactgca tcaatccgtt ttgttgtaat tttgggaaag aacagcacaa
    15841 attcaaagat gtaaaaggga aaccaagtcg atataaaatg actggtagtt ccaaagataa
    15901 aggtcataaa ggtatgtact gcaatgacaa tcctattggc agaggtgtat cgcaaaactg
    15961 tacagtaacc cctttatcta attggtctgt tgttgaagaa attaaacgcc ttatagaaat
    16021 caatagcatc caagatgttg agccagatta tcaatttcat aaagaaggct gctcggaaga
    16081 agaatcaacc ccattcaatg agccgaagca attttacaaa agggggaaaa gtaggggcaa
    16141 atctcaacgc tatcaatgta aggcgtgtaa gaagttcact aatgttctac caaaaagaga
    16201 agaaaccact acttatcacc aacaaaagaa caccattctt ccaatgttcg ctaagatggt
    16261 tgttggaagg gtttcagtca gtcgtacttg tgatatttta ggaattggag ttggaaccta
    16321 ctatcataag ctggagtggc tgtataggcg ttgtttggag tttttagaac gatatgagac
    16381 acaacccctt caaacaaaga aatttaacga gatgtggctt aatacggaca agatgcacta
    16441 ctacctaaat aacgtccgta agaaaggaca aggttcaaag aaatatacgg ggtttgaaga
    16501 ccttaatatg caaacgtata tagttgtatc tgctgaggtc ttatcaagat atgtgttccg
    16561 ttctgatgtt gcctatgatt ggaatatttc aatggatgaa ttaaatgaag atacaagaaa
    16621 attcaaagaa gaccatctca atacttttag tcgtaaaaat gataggttag attggtctta
    16681 ttatccacaa gaaccatccg ctaacgatag cgaaaatagg aatgcttatc ttcacgaatt
    16741 agggaaaatt actaatagga gtagatttgt cgatggttta aatgttgatg ctccttatac
    16801 tacaactgct cactattggt taattaaaca aatggttaat gccgacgaat ggcgaatgat
    16861 tagtgatgat gacttctcta ttagaaacgc cttttataga gtattcacga aagaattaag
    16921 gttgagcgat gcacatcatt ttatttgcca agttaacaag acgaaaagca gaaaacaatg
    16981 cttaaaagaa ttcggacaag caaaagcaga actgctcgat tggggagata tcagaggatt
    17041 caaaacaaag tttctacgga ctttggcatc tcattatcta actgaattac taacaagtca
    17101 tcaattccac gaagaggcta ttagtaaaga tggagaaaga tatcgcaaat atgccgataa
    17161 ccctatcaaa catccattag ccactaagga taaaggattt tattcagttg attgtagaac
    17221 ggatttatct gctctggaac caaacgaaat cgcaaagatg ctattaaatg ttaatgacca
    17281 ttcgaccaat agctttattc aacagataag acgttatata tcatctcttg aaagaccatt
    17341 aacaacagct cgtggtgata agaagagcta tatttacgct aacttcaatc cgaagtatgc
    17401 tcaatttgcg attacaattc taagaactta ctacaacttt tgtcgccctt ttaaatcagc
    17461 ggataaaaag gttttgacac ctgctcaaag attagggatt acagataaac aatttgattg
    17521 gaaagatata atttatttca aatgacaaaa agagcttgga gtaatcccaa gctcttgtgc
    17581 tttatcttta ctgttcttct tcaattaacg cacccgttaa ctcaataagt tttttctttc
    17641 atatacatga aaatagtagt tatacggatt tttatcattc tttatccctt tttgggcaaa
    17701 tacctcattc cattcctcat aattcacttc tggaaaaaaa gtatctcctt cgaattcatg
    17761 atgtattttt gtgatgtaca ttttctcaac ataagggaaa aacaaattat aaatctgttc
    17821 tcctccgaaa ataaaaattt cttcttcgtt tttacataac tcaaaaacat cttctattga
    17881 atgaacaatt tcacaaccat taaaggtaaa ccccttatct ctcgtcagaa taatatttct
    17941 tctgtcaggt aaggctcttc cgattgattc aaggttcttc ctacctaata ttatcggatg
    18001 tccctttgta gtatttttaa catattccca gtccttggga atcctccaag gaatgtcatt
    18061 ctctttgcca atcactctat tcttatccat cgcagcaatc aaagaaactt tcatttataa
    18121 cacagccttt ttaactaatt taccatctaa tttcttattt ctatcaaaaa accttttctc
    18181 attatcctgc ccgttagttg aataagcaat tttttctata agttatatta tcataatacc
    18241 acaaaatcac aaattttcat tcaattatca ttttcaattg cattttataa atcattttgt
    18301 atgtaatttg gtatgtaagt taaaatgaaa aaagtagtcc attttgaagt acattttgtc
    18361 attttgtact ccaaaactga actacttact tgcatatata cgcctttata gtggagtact
    18421 acatttacga gattcggtta gagtatcaga aaaagaaaaa ataaaagtta cagaaatgta
    18481 tacttcaata aatggtgaat tatgtaagat tgatagagct tattctggag aaattgttat
    18541 tttgcaaaat gagtttttga agttaaatag tgttcttgga gatacaaaac tattgccaca
    18601 gagaaaaaag attgaaaatc cgcaccctct actacaaaca actgttgaac cgagtaaacc
    18661 tgaacagaga gaaatgttgc ttgatgccct tttggaaatc tcagatagtg atccgcttct
    18721 acgatattac gtggattcta cgacacatga aattatactt tctttcttag ggaaagtaca
    18781 aatggaagtg attagtgcac tgttgcaaga aaagtatcat gtggagatag aactaaaaga
    18841 gcctacagtc atttatatgg agagaccgtt aaaaaatgca gaatatacca ttcacatcga
    18901 agtgccgcca aatcctttct gggcttccat tggtttatct gtatcaccgc ttccgttggg
    18961 aagtggaatg cagtatgaga gctcggtttc tcttggatac ttaaatcaat catttcaaaa
    19021 tgcagttatg gaagggatac gctatggttg cgaacaagga ttatatggtt ggaatgtgac
    19081 ggattgtaaa atctgtttta agtacggttt atactatagc cctgttagta ctccagcaga
    19141 ttttcggatg cttactccta ttgtactgga gcaagccttt agaaaagctg gaacagaatt
    19201 gttagagcca tatcttagtt ttaaagttta tgcaccacag gaatatcttt cacgggcata
    19261 taacgatgct cccaaatatt gtgcaaatat cgtaaatact caactgaaaa ataatgaggt
    19321 cattattatt ggagaaattc ctgctcgatg tattcaagat tatcgcaatg atttaacttt
    19381 ttttacaaat gggcttagtg tttgtttagc agagctaaaa ggatatcagg ttaccactgg
    19441 cgaacctgtt tgccagaccc gtcgtctaaa tagtcggata gataaagtaa gatatatgtt
    19501 caataaaata acttagtgcg ttttatgttg ttatataaat atggtttctt attaaataag
    19561 atgaaatatt ctttaatata gatttgaatt aaagtggaaa ggaggagatt gttattataa
    19621 actacaagtg gatattgtgt cctatttgtg gaaataaaac aagactacga atacgagtgg
    19681 atactatact taaaaatttc cctttatact gccccaaatg taagaacgaa actttaatta
    19741 atgttcaaaa aatgaatata ataacaatca aagagccaga cgccaagacg cagagccgat
    19801 aatttgagaa atgaaactct catcttatcg gctctttttg tttatctgaa ttttactgac
    19861 tagccttcaa tatttcttgc ttcattgata ccttttgcta gtgcttccat taaggatagt
    19921 tctttgtctg taaagctatc catgtatttt tctacctgta accttcgagt gcttttaatc
    19981 aaattatctg taggtaagaa aaattcatca atagatacat ggagtaatga tacaagatca
    20041 tacagcactt gtgtacttgg gtgttgtcct ttgttttcta tatttgttaa ataacgtggg
    20101 tcaatttcaa tcattgttcc cacttgttca cgagtgagac cccgtttcat tcgagcctct
    20161 ttaatcgcta aaccaacggc tctaaaatcg tatttatctt cttttttacg catactaaat
    20221 cacctctata cattttactg ttcctatata accataaaca ggtatagaaa aacatgttat
    20281 atagtttatg agttcctata gaacactgtg gaatttatta atgcgatgaa tccaatttca
    20341 aatagaaagg tcgctgattt tatttaccta tacaacttta taaaatttag tcatactcct
    20401 agaaagtaga taaaattggc gacttaatgg agggaaaagt cgccatgaga aagataatct
    20461 gtcatctagt tctcttaatt aagttcttat caaaaaaact tatccacctg tactggatag
    20521 atatgaccat caatgtggtt ttgcagatta catagataga aaaatcaaag catatgaaca
    20581 gagaaaacaa tctgtttatg tgcttttttg gtcacttaat aaatatcttt taggatttct
    20641 cttcatttat gcaacaaatc ccccgttcgt gtcggggttt agtatgaagg gagataaaga
    20701 agcacatgcc actccctttc acgacagaaa gggggtgaaa agcatgaaac catcttcttt
    20761 tcaagaaacc atagaaaatc agtttgacta tatctgcaaa caagttattg aagatgaacg
    20821 aaaagattac ttcaaacatc tagacagact atcagcaaaa gaagtctcgt tttctgatat
    20881 agataactac cttgtcaact ctttttctac tgttgatacg tacgtcactg atttccaact
    20941 atttaaatta tttggattaa gaatcaatgt tgaaagtgat ttattaagtg aagccctgcg
    21001 tcatctttca gagaaaaaac ggaacattat tttacttcat tactttatgg atatgagtga
    21061 tgtggagatt gcggagttac taaatttgaa tcgttcgact gtttatcgtc atcgaataag
    21121 tggactagcc atgattaaag aattcatgaa ggagtgtaaa gaatgaaaac agcttatcca
    21181 agggttccct tccccctcat tgtaaaggcg acagatggcg acgtggaagc aattaatcag
    21241 attgttaaac attatcgagg gtatacctca aaacgttcat tacgtcgcat gacagacgaa
    21301 tatggaaatt ctcatatggt tattgatgaa accctacgtg gcagaatgga aacgaaactc
    21361 atctctaaga ttttatcgtt cgaaattaaa tagtcccatt ttttcgtgga agcgtgtcga
    21421 atcgtatcca cgcttcccga tttgggaggt ttgttattcc atcaaagcat attgtgtgtt
    21481 cagtaggttt tgataggcta acaagccaaa cgttctttga caaccaaata aaggcaatcg
    21541 aatacgtttc ggtaagaaaa gagctgatga actaacacgc catgacctac aaaattagtg
    21601 agcgattcat gttagtaatt cgaaagtaat ctttagcagg attgctgcca tgactttctt
    21661 atcgtgataa tgatactccc gtatagccat agtccgagcg ttagaagcgt cacaggcaat
    21721 gggtacggct acatgagaac catgcagaag tgaaattcct gtgagctttg ctagagctgt
    21781 tcgattgcta agatattctt ctcttgtcag tgtattatta taaaactatt tgatataatt
    21841 ataggtgaat attggagttt tcctagccta taattgggaa aggagtaata atgataacgg
    21901 tcacaaaaaa ggatttagtt gagctaggat atggaacttc tttttcagca gacatcataa
    21961 aaaaagcaaa agaattgatg attacaaaag ggcatatcta ctatcaatct cgaaagctag
    22021 atagagtccc tcgtgaagct gttgaagaaa ttctaggaat tcggttttca aatgaaaaca
    22081 ggtctgagta gtttttgcac agtaaggagt aaaaactatg gttaaagacc caataaaaaa
    22141 agcaaagaat ggaacctatt attttcgtgc aaatctaggt tatgattcta aagggaaaaa
    22201 aattcaaaaa tatcgtagtg gattcactac taaaaaagaa gcaagagaag aatattcgaa
    22261 actgctattg atgaaaccag aagaattaag tgagaataag gataaaatga cgtttaaaca
    22321 ttatatcttt gaaattttcc ttccatggta taagacacag gtaaagctca gaacatatga
    22381 aaatcgctta ccaactatta agaagcattt ctcttatttt gataaaatgg ctgtttccga
    22441 tattgagcct attgatgtgc aaaattggca actaaaatta tcaaagaagt gtaagtcttc
    22501 ttatgtaaga gctgttcaag gactattttc agttgctatg gatcgagcaa ttgtccttgg
    22561 gataaccacc acaaatccat ctaagattat tggcaatgtg aaaaagcaaa agtctaaaat
    22621 tgaattttgg acaaaagaag aatttgaaaa agtaatctct ctaatttaca aagaagatta
    22681 ctatcaacac tttttattta tctctctgtg gtttttattt atgactggaa tgcgaattgg
    22741 tgaagctact gccattcaat gggaagatat tgattttgac tcaggagtac taaccatcaa
    22801 caaaacactc tattacaaga atcaatcgaa ttatagattt gttgaaccaa aaacaaaggc
    22861 gagcgttcgt catattgtat tagataaatg cacattaact tatttatccg aatggaagga
    22921 agtacaacaa aatctaattc agacagactt tgtaatgagc tataacggta ttcctaccca
    22981 aaaacataca ctatcatatg ctattgaacg ctatgctaag ctggctggta tccatcgaat
    23041 cagaatccat gggttacggc attctcatgc ttccttgtta atcagtatgg gagaaaatcc
    23101 acttatcatt aaagatcgtc taggacacga agatattgag acgactttag gaacgtatgg
    23161 acatctttat cctaacagta atttcgaggt agctaacaaa ttaaatggag ccatttcctt
    23221 taaagaagca aaacaaaatt atgatacttc accaaagaat caatttactg tagactactt
    23281 gcgaaacaaa gaaatgaaaa aagtgcaata aaagtgcagt aaaaagataa gaagagccag
    23341 aaacccttgg tataagagga tttctagctc tcctacaact attcccactc cactgttgct
    23401 ggtggtttag aagtgatgtc atacacgata cggttgacgt gggctacttc gtttacgata
    23461 cgtacagaga ttttttgcaa gacttcccat gggatacggg caaagtcagc ggtcattccg
    23521 tcgattgaag ttacagcacg gataccgact gtgtagtcgt aagtacgtcc gtcacccata
    23581 acaccgacag agcggatccc tggaagaaca gtgaaatatt gccagatatc acggtcaagt
    23641 ccagcagctg cgatttcttc acgcaagata gcatcagatt cgcgtacgat ttctagcttg
    23701 tcttcagtga tttcgccaag aacacgaata ccaagaccag gaccagggaa tggctgacgc
    23761 caaacgattg aatcaggcat gcctaactga gtccctaatt cacggacttc gtctttgaat
    23821 aatgtgttta atggttcgat caattcaaat tgcatatctt ctggcagtcc acctacatta
    23881 tggtgagatt tgatcgtctg tgctgtttct gtaccagatt cgataacgtc agtatacaac
    23941 gttccttgtg ctaggaaaga gacgccttct tcaccagcaa gttttgttgc ttcgtcatca
    24001 aaaacatata cgaattcatt cccaatgatt ttacgttttt gttctgggtc agatacacct
    24061 ttcaacttgt ctaagaagcg atctcttgca tcgactttga tgatgttcaa gccgaatttt
    24121 ccgcctagac tttccattac ttgttccgct tcgcctttgc gtagcagacc gtggtcaaca
    24181 aagatagatg tcaattggtc accgatcgct ttttgtaaaa gaaccccaac gacactagaa
    24241 tcaacgccac ctgaaagacc aagcaagact tttttgtcac caacttgttc acggattttt
    24301 tctacttgca tatcgataaa gttatccatt gtccagttac cttcacagtg acaaacatca
    24361 aaggcaaagt ggcgcagcaa ttcgattccg tattcggaat gacgtacttc tgcatggaat
    24421 tggataccat aaaaattacg tgttgtgtct tggatagaag caatcggaca atctttgctt
    24481 gttccgactg tttcaaagcc ttcaggaact ttagttacca agtcaccatg gctcatccat
    24541 actgtttgtt gttttggtgt ttctttaaac aagacagctt gatcgcttgt tacttctaac
    24601 aaggctttcc catattcgcg gtttttagca ggctctacgg aaccgccaag tttgtacgtg
    24661 atcaattgca taccatagca aatccctaat acaggaatac ctaaattgaa gatctcagga
    24721 tcgatcccaa atgcattctc atcatataca ctgtttggtc cgccagaaag aatgattccc
    24781 tttggattca tcgcttttac ttcttctgca gtaatgcggt ggctcaataa ttcggaaaag
    24841 actccaagat cacgaatacg tcttgtgatc aattggttat actgactacc gtagtctaat
    24901 acgataatct tttcaacgtt cgtcatatcg gcaacgtttg tcaaatttat tacccctatc
    24961 cctttaaatt ttatctcaaa atagtcaaac tattttaaaa tgctaatatt ttcgcatgaa
    25021 aatcgaatcg atgatatgat tttcagtttt atggagaatt atatcggctc ttcctcgtgt
    25081 aggcaagata tattcctgta aatttttcaa attcacattt ttccatacat cttttgccat
    25141 tttaaaggct tcttcacgat ctccgatagc aaaacgataa taataattct ctggattttg
    25201 gaaagccgta tccagtaacg caccaaaacg atccaaatac catttttcga tcagcacggg
    25261 atcggcatcg acatagacag aaaaatcaaa aaagtcgctg acgtagattt gctgattcgc
    25321 aggtaattga agcgtattga tcccctcaac gatcaagata tcaggctgct ggatcaattc
    25381 gtacttgtct ggaatgatat catacacatc atgtgagtaa acaggtactt ttatctcttc
    25441 ctgaccagat tttacacggt tcaagaaatc gatcagcatc tccatatcat aactttcagg
    25501 aaatccttta cggtccatga ttccacgctc ttttaatata cgatttggat aaagaaatcc
    25561 gtcagtcgtg atcagctgaa cgtttcttcg tttgaaggta cgagacaaaa tcgtctgcag
    25621 cagtctggct gttgtacttt ttccaacggc tacacttccg gcgatcccga taataaaagg
    25681 cggtacttct acgtatttat gaagaaataa acctttactc aagaacaaag attcaaattc
    25741 tttcatataa agataaatca gatgagcaag aggtacatag atatctcgaa catcctgcat
    25801 ggaaatgcgg tcattcaaac ttttgatatt atctagttca tcttgtgtca aaggtgcttg
    25861 cccattacga taaaattctc gccattcttc tcttgccaca cggtagtagt tcattggatc
    25921 attcatatta gcctcctgcg ttctcccatc aatggaacta ttttaacaca aaataaacgc
    25981 aaaccccacc acttaagaga gattgccgat ctttttgtat aatctttata atagaaagaa
    26041 acggagtctt attcttgact tgcgcacgga tacataaaag gatagactgt tgtcatgaaa
    26101 gaaatgagaa ggatggaaga catgacaaat cattactatt ctgaacgccc agatacggcg
    26161 cacgattttg aacaatgggc ttttgagtta aaaggcagaa cctttcaatt tgtaacagat
    26221 tctggtgtat tttcacgtga aacagtggat tatggttcac gtgtcctgat tgatgctttc
    26281 gattgggaag agttacctga tgaaggaaag atcttagatg tgggatgcgg gtatggaccg
    26341 attgggttag ctttggcatt tgcgacacag cggtttgttg aaatggtcga cattaatgaa
    26401 cgagcggtaa gtctagcgca aggaaacgca aagcgtaacc aaatcgaaca tgttgatatc
    26461 catcaatcga atatctatga agcagttcat gaagaaacct atgcagctat cgtaagcaat
    26521 ccgccgatcc gtgctggaaa aaaagttgta catgaaattt tgacaggcgc atatcctcgg
    26581 ttgaagaagg gcggtacact gacaattgtg atccagaaga aacaaggagc gccgagtgcg
    26641 caaaagaaaa tggaagaaac atttggtaat gcagaaatcg tcacaaaaga taaaggatat
    26701 tatatcatca aaagtgtgaa agaataacta gctgaaaaat agtggagcga cgaaagagtg
    26761 gaactgggcg tgcatgtacg ggtacgtatc agttcttttt ttgttgagaa gtttttaaaa
    26821 acaaagaact attttttctt caaaaagaaa tagtaggaac agagtcgttg ttttttacga
    26881 taaaataaat aagatacaga acttgtatag aaggctgcca agtcggtaga taaaggaggt
    26941 ttttatcaaa aaacatactt tccttttttc tctattcttt tcttgggggt tttggtacac
    27001 tgatagggaa tttagataga agggagccaa taggatgaag ctgaccatca gagatatcgc
    27061 agaaatggca ggcgtatcag taacaactgt ctcacagatt ttgaataaca aaggaagcag
    27121 atttagtgat aagacaagaa aaaaagtgtt gtcgatcgtg aatgaacatc attataagcc
    27181 cgattatttc gcttcaaatg tgatcaatcg acattcaaag accatcggaa tgatcgtccc
    27241 tgatgtcact gatttcttct tctccaaact gatcgaaggt gtagagagct atttgaatcc
    27301 tttgggctat gtgatcatgt tatgcaattc tcgtcatagc caagaaaatg agatcaagta
    27361 tttacaagag ctttcccacc gctcagtgga cggaatcttg ctggccacac caaatatttt
    27421 acctgagaaa tatgcattag acagcggttt ttatcaaaaa atgccaatca ttttaatcga
    27481 tcgcggattg aatcgtcggg ataacggacg gcttatcgta aaggaatatg agggcgccta
    27541 tcaagctgtt tcgctgttta ttgaaaatgg ccatacgaag atcggcatgc tgaaggaaac
    27601 cactggatac tatcaattag aagaacgttt caacggatat cgtcatgcgc tgaaagatca
    27661 tggcctgcct tttaatggga aatttgttga acatggagag ttgactgtcc aaggtggata
    27721 tactgcatca aaaaaattac tcaaacataa agatatcacg gctatttttt gcgggaatga
    27781 tgccatggcg atcggatgct atcaggcgat cgatgaattg ggcaaaaaaa tcccagaaga
    27841 tatctcagtc ataggattcg atggattaaa gctgtcagaa tacatgattc cgcaattgac
    27901 gacagtgcag cagccaagtt ttgatattgg attttatgct gcacgtttcc tgatcgatac
    27961 gatcgaattt cctcaaagaa aagttccgaa caaagtgttc gaaacaaaat taattattcg
    28021 tgagagcgta aaagtattga caaagggcta actcggcggt atattgctta tgtaagcatt
    28081 tacaaaggct agttagtgct ttttttattt ttagtaaacc agtttactaa agtggtttac
    28141 ctttcctaaa aataatgtat aataccgaat gaagacaagg aggatcaggc aatgcgaatg
    28201 gtggatctga ttgaaaaaaa gagggatgga aacgagctgt cgaaagagga aatcgagtac
    28261 atcgtcacga actatacgaa tggaaagatt ccagattatc aagtcagtgc attattgatg
    28321 gcaatttttt atcaagacat gacaaatgaa gaaatcacga acttaacatt ggcgattgct
    28381 aattctggtg atgtcatcga tttgagttct cttgaaggaa taaaggtaga taagcattcg
    28441 acaggtggcg ttggtgacac gaccacattg attttagccc ccctagtagc tagtgtcgga
    28501 gtgacagtgg ctaaaatgtc tggacgcggt ctaggttaca caggtggtac actagacaag
    28561 ctagaagcaa ttcccggatt tcagatcgag ctttcggatg aagaatttgt acgcattgtt
    28621 aatgaaagta aagtagcggt aattggtcaa tcaggaaact tggcaccggc ggacaaaaaa
    28681 ttgtatgcct tgcgtgacgt cacagcgacc gttgattctc ttccgctgat tgccagttcg
    28741 attatgagta aaaaaatcgc ggcaggagca gatgcaatcg ttttggatgt gaccactgga
    28801 gacggtgctt tcatgaagaa catcgaagat gcaagacgtc tggcaaagac catgacgagc
    28861 attgggaaac tggctaaccg agaaacagtg gcggttatct cagatatgtc agaacctctt
    28921 ggcgaagcta tcggaaatag tctagaagtt gtcgaggcaa tcgaaacatt acaaggaaat
    28981 ggtccagaag atctagtaga aatgtgctac gccttaggaa gccaaatggt tgttcttgct
    29041 ggcaaagcta agacaaccga tgaagcaaga acgttgcttc aagaagctct ggaatcaggt
    29101 aaagcgttag caaaattcaa agaaatgatc caaaatcaag ggggagaccc agcgatcgtg
    29161 gaacatccag aacgaatttt gactgcacgc tacacaatgg agcttcctgc taaacaaagc
    29221 ggagttgtgt caaaaatcgt ggcaaatgaa ttaggaattg ctgcaatgat gctaggtgct
    29281 gggcgtaaaa ccaaagaaga agatattgat cacgctgttg gattgaaact gcataaaaaa
    29341 attggtgaca ctgtgacaaa aggtgagtca ctattgacga tctatagtaa cgacgaagaa
    29401 atcacttctg tcattgaatt attatataaa aatattgaaa ttggtgaatc ggcaatgaag
    29461 ccaacgttga ttcatgacat catcacagaa taaaggaaaa aagcggactt taccgtttta
    29521 aagaaaaggg gaaaagaaat ggaactaaac cgaatgatcg accatactat tttgaaagca
    29581 gacgcatcta aagaagctgt gatgcaaatt atcgaagaag caaaaaaata ccatttttat
    29641 tctgtctgca tcaatccagc atgggttgcg ctagcgaaag aacaattaca aggtgagcca
    29701 gtagccgtat gtacagtaat cgggtttcct ctaggagcaa acacacccga gacaaaagca
    29761 tttgaaacaa cagatgccat caataacggt gcagatgaag tcgatatggt catcaatatc
    29821 ggtgctttga aatcaaagga tgatcaacaa gtccaaaaag atatcgaagc agttgttgaa
    29881 gcagcaaaag atcgtgcgct tgtcaaagtt attattgaaa catcgttatt agatagagaa
    29941 gaaatcatcc gagcttgcga aatagcaaaa gctgcaggcg ctgattttgt caaaacatca
    30001 actggtttct caacaggcgg agcaaaggta gaggatgtcc gtttgatgcg tgaaactgta
    30061 ggaccagaaa tgggtgtcaa agcttccggc ggtatccata acgaagaaga agcaatggca
    30121 atgatcgaag ctggtgctac aagaatcgga acaagcgcag gagtagcaat cgtatctggt
    30181 tcaactggtg aaggctatta attttttcaa aggagaagct catggcagaa ctcaaacaag
    30241 aatggatcaa cacagctatt gaagcgttag ataaagccta tgttccttat tcccattttc
    30301 cggtaggcgc ttgtctcgta acggaatcag gcaagattta tcaaggcatc aacattgaga
    30361 atgcatcatt tggattaacg aattgtgccg agcgcacagc ttttttcaaa gcagtatctg
    30421 aaggagaaaa aacatttacg catctagtcg tagcggggca cacccccgat cctatctctc
    30481 cttgtggtgc atgtcgacaa gtaatggcag aattctgttc ccctgatatg ccggttacgc
    30541 tagtaggaga caatggtgtg acgaaggcaa cgaccgttcg tgcactgctg ccttatgcat
    30601 ttactgaaaa agatctataa ctcatgttca ttcagtcagc tgactgttga aaaataaaac
    30661 aattatttta ggaggcttta cagaaatgaa aaaagcaaaa ttatttggtt taggtgctgt
    30721 cgcactagca gcagggttat ttttaggtgc atgcggcaac aacggctcta ctgattcatc
    30781 cggtggcaaa agctcatcag acacaacaac tgcagcattg atcacagaca caggtggtgt
    30841 agacgaccgt tcattcaacc agtcagcttg ggaaggacta gaaaaatggg ggaaagatca
    30901 aggactttca cgaggaaatg atggcttcca atatttccaa tcttcaaatg aatcagatta
    30961 catcccaaat attgaccaag cattgaatgc tggattcaaa acgatttttg gtattggtta
    31021 taaactaaaa ccagcgatcc aagaacaagc aacaaacaat acaggtacga actttgttat
    31081 tatcgatgat gtcatcgatg gattagataa cgttgtatct gcaactttca aagacaacga
    31141 agcttcttat cttgcaggtg ttgcagcagc ttatacgact gaaacaaacg tagtcggctt
    31201 tatcggcggt gtaaaaggtg aagtaatcga tcgttttgat gctggattca aagccggtgt
    31261 tgatgcaggt gcgaaagaat taggaaaaga aatcaaagta ttgaatcaat atgctggtga
    31321 cttctctgct ccagataaag gacgttcaat tgctcaaggt atgtatgcac aaaacgcaga
    31381 tatcatcttc catgcttcag gcggtacagg taacggtgta ttccaagaag caaaatcatt
    31441 gaacgaatca ggcgacaaaa aagtttgggt tatcggtgtt gaccgcgacc aatcagacga
    31501 aggcgaatac acattaaacg gcgaaaagaa aaacttcaca ttgacatcta cactgaaagc
    31561 agttggaaca gtagtagaag atttggcaca aaaatcagcg gacggtaaat tccctggcgg
    31621 cgaacacact gtttatggat tgaaagaaga cggtgttggt ctaacagaag gacaactttc
    31681 tgacgaagcg aaaaaagcag ttgatgaagc aaaagaaaaa atcatttcag gtgacgtaaa
    31741 agttccagaa actcctgaag aaaactaaaa ccatttctat agccttgtta gacacatgtt
    31801 tgacaaggct atggtttgat tagaacaaga attgttcatt tatccaagct gccttgtgac
    31861 agtttggata agggaacaac tccctgaagt aatgtttgtt tcaaaagaag aacgctcact
    31921 aagcgtgaat cttagtcaat cgttgcccta gatagaaatc gttaggatgt gaaaaaaagg
    31981 tggttcaaga aaatattgtc atagaaatgc agaatattac caaagaattt ggaacattca
    32041 aggcaaatga caacatcaat ttacaagtaa aggcaggaga aattcatgcg ttgcttggag
    32101 aaaacggtgc tggcaaatct acattgatga acgtgctttc cggattatta gagccgacat
    32161 cagggaaaat tttgatgcgt gggaaagaag taaagatcac aagtccgacc aaagccaacc
    32221 aattagggat tgggatggtc catcagcact ttatgcttgt tgatgccttt actgtaacag
    32281 aaaacatcgt gttgggaagc gagcctagtc gcgcagggat gcttgaccat aaaaaagcac
    32341 gaaaagagat ccaaaaagtt tctgaacagt atggattgtc agtcaaccca gatgcttacg
    32401 ttcgtgatat ttcagttgga atggaacaac gggtagaaat tttaaaaaca ctttaccgag
    32461 gagcagatgt actgattttt gatgagccga cagctgtatt gacccctcaa gaaattgatg
    32521 aattaatcgt gatcatgaag gaattagtca aagaaggcaa gtcgatcatt ttgattacgc
    32581 ataagttaga tgaaatcaaa gcagtagctg accgctgtac agttatccgc cgtggaaaag
    32641 gaatcggtac agtcaacgtt aaagacgtaa cctcacaaca attagccgat atgatggttg
    32701 gaagaacggt ttcattcaaa acgatgaaaa aagaagcgaa gcctcaagaa gtcgttttgt
    32761 ctattgaaaa tctagtggtg aaagaaaatc gtggattaga agccgtgaaa aacttgaact
    32821 tagaggttcg tgctggcgaa gtacttggta tcgctggaat cgatggaaac gggcagtcgg
    32881 agttgatcca agctttgact ggtttgcgaa aggcagaaag cggacatatc aagctaaaag
    32941 gggaagacat cactaataaa aaaccccgaa aaatcactga acatggtgta ggacacgtgc
    33001 cagaagaccg tcataaatac gggttagtcc tagatatgac attatctgaa aatatcgcac
    33061 tacaaaccta tcatcaaaaa ccttacagta aaaacggcat gctgaattat tcagtaatga
    33121 atgaacatgc cagagaattg atcgaagaat atgatgttcg aacaacgaat gaacttgttc
    33181 ccgcaaaagc tttatcaggc ggaaatcagc aaaaagcaat tatcgctcgt gaagtcgacc
    33241 gagatcctga tctgttgatt gttgcaaatc caactcgtgg actggatgta ggtgcgatcg
    33301 aatttattca taaacgtctg atcgaacaaa gggacaaata caaagcagtg ttactgatca
    33361 gtttcgaatt agaagaaatt ttaaatgttt cggatcgtat tgctgttatc catgaaggag
    33421 aaattgtcgg tatcgttgat ccgaaagaaa catctgaaaa tgaattaggt ctattgatgg
    33481 cgggctatac cttagaagaa gcacgaaaag agttagaaaa gggggctggc gcacatgaat
    33541 gatcgcagcg aaaaattccg taatttatta gtgcctgtac tttcagttgt tttaggattg
    33601 atcttaggcg cgatattgat ggctgctttt ggttttcagc ccatccaagg ttattcttct
    33661 atgctagatg cagcccttgg ttcgcaaaga gggataggtg aaacgttaag gcaagcaacg
    33721 ccgttgattt ttacagcatt aggtttttct gtagctaact cagctgggtt tttcaatatt
    33781 gggctttctg gacaagcact ttgcgggtgg atcgtcagtg tctgggtggc acttgcgttt
    33841 ccagatctgc caaaagttgt tttgcttccg ttatgcgtga tcttaggtgc cttagctggt
    33901 gcgatggcag cggctgttcc aggtttactt cgagcatttt ttggaacaag tgaagtgatc
    33961 gtaacaatca tgatgaacta tatcttatta tacgtaagca catttgcttt acatgaagtg
    34021 atgcctgaaa gctatcgctc aagtttggac tcttccaata tgatcagtca aaatgcctca
    34081 ctgcggttac catggttaac acaagctttt ggcggctctc gcgtaaactc aggattattt
    34141 ttagcaatca tcgcgcttat tttcgtttgg ctgctgatga agaaaacgac tttaggattt
    34201 gaaatccgtt ctgttggatt gaatcctttt gcttctgagt atgcaggaat gagtagtaaa
    34261 cgtacgatcg ttttgtcgat ggtcatttct ggcgctttgg cagggcttgg cggtgtggtt
    34321 gaaggattag gaacgtatca aaacttcttt gttcaaacga cttcgctatc tattggattt
    34381 gacggaatgg ctgtttcctt gcttggttct ggatcagcaa tcggtattct tttatcagcg
    34441 atcttgttca gtattttgaa gatcggtgga cttggcatgc agacaggagc aggtgttccg
    34501 tttgagatcg tcaatgtatc gattgctttg attatcttct ttgtcgggat caacttcctg
    34561 attcgtttcg tcatggctaa agtcttccaa gggaaaaaac aagaagagat cgttgcgaca
    34621 atagaatcga aaccaacgaa tcaaaaccat gaagggggcg aactataatg tcgactatcg
    34681 aattaattgc cttgatcctt acttctacac tggtttattc aacaccgcta attctaacgt
    34741 cactaggcgg aactttttct gaacgaagcg ggatcgtaaa cgtagggcta gagggaatca
    34801 tggtcatggg tgcttttagt tccatcgttt tcaacttgac gatgagtcca tcacttggtt
    34861 ctatgacacc atggatcgct attttagtgg gtggcgtcgt aggtatccta ttttctctga
    34921 ttcacgcagt agcaacagtc agtttgcgtg ctgaccatat tatcagcggg acagtaatta
    34981 atctgatggc acctgcttta ggtgttttct tgatcaaagc atggtatggt aaaggtcaaa
    35041 ctgataacat cacgcagaat ctggggtatt tttctttccc aggattagca gatattccgg
    35101 tcattggacc aattttcttt aaaaatacat tcttacctgc ttattgtgct attttgattg
    35161 ctgtcctagc ttgggtgatc atcttcaaga cgaaattcgg cttgcgcctc cgttcagttg
    35221 gtgaaaatcc tcaagctgcc gacacattag gtatcaatgt atatgctatg cgttattcag
    35281 gcgtactgat ttctggcttg ttaggcggta tgggcggtgc tgtttttgct cagtcgattt
    35341 ccggtaactt ttcagctggt acgatcgttg gacaagggtt catctccatg gctgctatga
    35401 tctttggtaa gtggaatcct ttaggggcaa tgggtgcctc attgttcttt ggttttgctc
    35461 aaagcttgag tatcattggc gcacaattac cagttatttc ttcgattccg ccagtattgc
    35521 tgcaaattgc tccatatttg ctgacaatca ttgtgctggt tgttttctta gggaaagctt
    35581 ctggtccaaa agcaaacggg aagaactata tcaaatcaaa ataatctttt agcgaataaa
    35641 aagggtgggt tacgggaagt gtttttcgtc tcgccctttt tgttttaaag gcaataaaaa
    35701 taaaaagaaa gaaggaataa tcatgtttaa acgcgtacat ctgatcgtca tggattcagt
    35761 aggaattgga gaagcaccag atgccgataa gttcggtgat gttggttctg atacattagg
    35821 acacattgcg aaagaagcag gattgaacgt tccacattta gagcagcttg gtttaggaac
    35881 gatccgtcct cttgaaggtg tggaatcagt gaaagatcat caaggctatg cgacgaaatt
    35941 agaagaagtt tctgtaggga aagatacgat gacaggccac tgggaaatca tgggattgaa
    36001 tatcaaaact ccatttcgtg tgtttcctaa tggattccct gatgatctga tcaaacaaat
    36061 cgaagaattt tctggccgta aagtcgtatg taacaaacct tatagcggaa cagctgttat
    36121 tgacgattac ggtgaacacc aaatgaaaac aggcgattta attgtatata cttctgccga
    36181 tcctgtcctt cagatcgcgg cacatgaaga aatcattcct ttagaagaat tatatcggat
    36241 ttgccaattc gttcgtgata taacaaaaga tgatccgtat atgattggcc gaatcattgc
    36301 acgtccgtac gtgggtgaac caggagactt cacacgaaca agcaatcgcc atgattatgc
    36361 gttgaatcca ttcggacaca cagtccttga ttctttgaaa gaagcaggaa aggacgtgat
    36421 tgctgttgga aagatcaacg atattttcaa cggacaagga atcactgaat ctgtccgcac
    36481 gaaaagcaat atggatggcg ttgaccagct gctgaaagtt atgaaaaacg atttttcagg
    36541 aatcagcttt accaatttag tagattttga tgcattatac ggtcatcgtc gggatgtagt
    36601 cggctatgcg catgcgattg aagaatttga cttacgaatt ccagaattgc ttgaagcgat
    36661 ggaagaagac gacttgctaa tgattacagc cgatcacgga aacgatccga cgtttactgg
    36721 taccgatcat acgcgagaat atgtaccgtt attggtttac agtaaaaaaa tgaaagaaca
    36781 aggggcattg cctcaaggat attattcaga tatttcagca acgattgctg agaactttag
    36841 tgtttcttcg actgaaaatg gcgaaagctt cttgaaatta ttaaaataat ctttttaaat
    36901 aggagaaagc aaatgaccaa gttaagcgag atgttacaag aaacgaccca gtttatcctt
    36961 gataaaggag taaaagaaat tgattttggt ttgattctag gttcaggatt aggtgaatta
    37021 gcagatgaaa tcgaagatgc tgtaatgatt tcttatgatg agatcccgtt ctttcctgtt
    37081 tcgacagtag ttgggcatgc aggaaagcta gtttatgggt ctctggcagg caagaaagtg
    37141 ttagccatgc aagggagatt ccactactat gaaggtcatt ccatgcaaac agtgacctat
    37201 ccagtacgtg tgatggccgg actaggggcc cattcagtca ttgtgactaa tgcatgtggt
    37261 ggtgtcaacg aatcatttgt accaggagat ttgatgctga ttaccgatca tatcaatttt
    37321 actggtgaaa atcctttgat cggtccgaat gaagatgcaa tgggccctag atttccagat
    37381 atgagtcaag cttatacgcc tgactatcaa gcaacagcaa aagcagcagc agaaaaacta
    37441 ggtttggatc ttaaagaagg cgtatatatg ggctattctg gaccaactta tgaaacgcct
    37501 gcggagatcc gaatgtctcg gacaatagga gccgatgcag tagggatgtc gactgttcca
    37561 gaagtcatcg tagcagctca cagcggatta aaagtgttag gaatctcttg tattaccaac
    37621 ttagcagcag gtatgcaagc gaatctgagc catgaagaag ttgtagaaac aactcagaga
    37681 gtcaaacaat cttttaaagc attggttaaa gaaacattga cattattgta ataggaggaa
    37741 caataaaatg agtgtgcata ttgaagcgaa agaaggcgag atcgccgata agattttact
    37801 tccgggagat cctttgcgag ctaaatatat tgcggagact tttttggaag atccagtttg
    37861 ttacaatcag gtacgcggta tgttaggctt taccggcaaa tataaaggag aacgtatttc
    37921 tgttcaagga acaggaatgg gaatgccatc agccaccatc tatgcccatg aattgatcca
    37981 gtcgtatggt gtaaagaaat tgattcgagt aggtacatgc ggtgcattgt caaaagacgt
    38041 ccatgttcgt gatttagtac tagcacaagg tgcagcgacc agttcttcga tgatcgaaaa
    38101 aaatttccaa gcctttcatt ttcctccaat cagtgatttc aatctattac tgaaagcata
    38161 tgagatagct aaagaaaagg gctatacggt tcatgtagga aatgttcttt cggaagattc
    38221 tttctacaaa gatgatttga cagagacgtt ccaattagct gaacttggcg ttttaggagt
    38281 ggaaatggaa gcagcggctc tttattactt aggcgcgaaa taccatgtgc agacattaag
    38341 tttgatgacc gttagcgacc atttgatcac cggtgaagaa acaacagctg cagaacgtca
    38401 atctacgttc aatgaaatga tcgaagtagg attggaaaca gcgatagcag aataagaagg
    38461 aaatgaaaaa gctgccctgc attaagtaat aagaacatcc aagcaaaaat agctcctcat
    38521 gtttttgact tggctggact tattattgca aatgtaagct tttgaacacc gtttaccaag
    38581 aggttgtgaa tctgccgttt aactctgagt cacaaggaac aatttcaaaa aacagctttt
    38641 catgtttgat gaatattgtg acttgtgacc gagaagttgg caggagaaaa ccgtttatac
    38701 gaaaaaaggg actgtgacaa atcgtgtcac agtccctttt ttatagtgtt ttttataggt
    38761 agtatttttt agcaactgtt aagtcatcat ttaattcata aacaagtggt tgacctgttg
    38821 gaatttcaag atccatgatg tcttcgtcag agataccttc gatatgttta gctagggcgc
    38881 gtaatgagtt accatgtgca gctactaaaa cagttttgtt gtctagtaat gctggagcaa
    38941 tttcatcttg ccagaatggc aaagcgcgtt ctaatgtcac tttcaagttt tctccaccag
    39001 gaatatcgcg tttgtctaac attgcgtaac gacggtcatt tgctgcagaa ccttcgtcag
    39061 tttcttccat taatggagga agagtatcgt aagaacgacg ccagatgtgc acttgttcgt
    39121 cgccgtattt ttcagctgtt tcttttttgt tcaaaccttg taatttacca tagtgacgtt
    39181 cgtttaagcg ccatgatttg atttgtggta cccaaagttg atctgaatat tctaagatca
    39241 agttacatgt tttgattgca cgagttaaaa cagaagtgta agcaacatca aattcgattc
    39301 cagcttcttt gattttgcgt ccgccttctt ttgcttcttc gattccttct ggtgctagat
    39361 ttacatcagc ccaaccagta aattggttca atgcattcca ttcactaaga ccgtgacgag
    39421 aaaaaactaa ttttggcatg tttagctctc cttttaagat aggtttatat ttctgaaatt
    39481 ttcagttctg ttttattgta caattttttg gaagagattt ccatcgaaaa attagataag
    39541 attctatgag aaaacaagta gagaatataa aaaaacgaac agtgcccacg caactgttcg
    39601 tttttaggta ttagatgcat aattgctcga tttctttttc aggtaaattg atatatttat
    39661 aacatgttcc aactgaaaca ccgcattttg aagaaataaa ttggatcgtt tctttttttt
    39721 cataatagag acgtcggatt tttttgatcg tttttgcatc gatttttggt cttccgccga
    39781 tttttccttt tttacgagct tcatctaatc caactaatgt acgttctttg atcaatgcgc
    39841 actccatgtt tgctaggttt tccattaatt ggaaatagat ttttcccatt tcgcgcgtat
    39901 cgattccttc atcaagacta atcaagtgca gcccgcgatc tttgaataat tgcgtgagtt
    39961 ctgttaattg ccgcgtagat ttgcctaatc gatgaagttg gcaaatgact agtgcatcgc
    40021 cttgttgcat gtcagcgatc actgactcca aatgaacagc agtagaatca ttttgttcca
    40081 cttcatatgt ctcatggaaa atttcttgac aaccaaagtc agataattga tcaacctgag
    40141 ttgttaaatc aacgtcattt atggttgtgc gtgcataacc aataattttc atggtgattc
    40201 ctttctagca gtgattttct aactggatat aacagtaaaa atgtagcata aagtacgtca
    40261 aaaagatagt tttttcgcaa attttccgct gtcttttttt attggatgat caagcagttt
    40321 ttctttattt acgacggatt gcgtcaagaa tctcaacgcc tttttctgag ccgacaacta
    40381 ctttagaaac catgtttagg aaaagcccat gttctactac cccaacaagt tcatctaaat
    40441 aggtagctaa gctttctggc tgttcgatga cttctagatg cagatcaatg atgtaatgac
    40501 caccatctgt ggttaagact tctccgtttt cgtctgtacg caagacgggt tggaatcttt
    40561 tttcttcaaa aatatgaagt agctgttggg agccataagg gatcacttca actgggagtg
    40621 ggaacttccc aagtttatcg actaatttag aagaatcaac gatccaaatc gtttcttttg
    40681 agtacgtagc gactattttc tcgaaaagca aagcagcgcc gccgcctttg atcccttgaa
    40741 aatcagcact gatttcatca gcaccgtcta ttgtcaaatc gactacgggg acttcatcaa
    40801 tactctttaa cggtattcct aatttttccg cttgttcttt tgtcgcattt gaagtagtaa
    40861 ctcctgtgat acgtaaacct tcttcacgca tacggcgacc aatctcttct accatataat
    40921 aggcggttga accggtcccg agacctacga tcatcccgtc ctttacgaat ttcgcggctt
    40981 caatgccagc taattgtttt aaattcatct ttgcgtccac ctttttttag tttgagcatc
    41041 gtgctttcct cactatttta gcgtgattta cggtaaaaag ataccattct tttttattcc
    41101 aataaagctt tttcaaaaaa aattgcatct cttgttgaca tgttttttca ttcgtgatat
    41161 tctaacaaag gtgctttatc tacaggtttc tgtgaagaaa cgtgctgact gtttatcaaa
    41221 gcatatttga taaggatgca gacattgcat ttatttttta tcacaaaaaa agaaccacct
    41281 ggatgtgtgg aactagacgc aaatcgtggg aaggaggaaa caaggaatgc ctacaattaa
    41341 ccaattagta cgtaaacctc gtaaatcaaa ggtggaaaaa tctaattcac ctgctttgaa
    41401 caaaggatat aacagcttta agaaaactca aactaacgta aactcacctc aaaaacgtgg
    41461 ggtagcaact cgtgtgggta caatgacacc taaaaaaccg aactcagctt tacgtaaata
    41521 tgcacgtgtt cgtttgtcaa acttaattga agttactgct tatatcccag ggattggtca
    41581 taacttacaa gaacacagtg ttgtattatt acgcggtggt cgtgtaaaag acttaccagg
    41641 ggtacgttac catatcgttc gcggtgcttt agatactgct ggtgttaacg accgtaaaca
    41701 aagccgttct aaatacggaa ctaaaaaacc taaagcttaa ttttaattat taaacaaatg
    41761 aaacttgctt ctactatata gaagtaatta ctatgaacaa tatttcggaa ggaggagtta
    41821 cggatgccac gtaaaggtcc tgttgcaaaa cgtgatgttt taccagatcc tatttataac
    41881 tcaaaattag taactcgctt gatcaaccgt gtaatggttg acggaaaacg cgggattgca
    41941 gctaacatta tctataatgc ctttgacttg atcaaagaat ctacaggtaa cgatccatta
    42001 gaagtgtttg aacaagcaat gaaaaacgtt caacctgttc ttgaagttaa agcacgtcgt
    42061 gttgggggtt caaactacca agtaccagtt gaagttcgcc ctgaacgccg cgttactcta
    42121 ggtcttcgtt gggtagtaaa ctacgctcgc ttgcgcggtg aacacacaat ggaagaacgc
    42181 ttagcaaaag aaatcatgga tgctgcaaac aacacaggtg cttctgttaa aaaacgtgaa
    42241 gacactcata aaatggctga tgccaaccgt gcgtttgctc actatcgctg gtaagattct
    42301 ctctgtttgg tcggctttta taagtcgacc tcagatagtg tccttatttt tagcaactag
    42361 atagtaaaga gaggagaaac aaggagaatg accagagaat tttctttaca aaacactcgt
    42421 aatatcggta tcatggccca catcgatgct ggtaaaacga ctgctactga acgtatcttg
    42481 tactatactg gtaaaatcca taaaattggt gaaactcatg aaggagcttc acaaatggac
    42541 tggatggaac aagaacaaga acgtggtatt acgatcactt ctgctgctac tacagcacaa
    42601 tggaagggtc accgaatcaa catcatcgac acaccaggac acgtagactt cacagttgaa
    42661 gttgaacgtt cattgcgtgt acttgatggt gctgtaactg ttcttgatgc acaatcaggt
    42721 gttgaacctc aaactgaaac agtttggcgt caagcaacaa cttatggtgt acctcgtatc
    42781 gtatttgcaa acaaaatgga caagatcggt gctgacttct tatattcagt atcaacttta
    42841 catgaccgtt tgcaagcaaa cgctcaccca atccaattac caattggtgc ggaagacgac
    42901 ttcacaggaa ttatcgacct tgtaacaatg aaggctgaaa tgtatacaaa cgatcttgga
    42961 acagagatcg aagaaacaga aattcctgaa gaataccgcg aattagctga agaatggcgc
    43021 gaaaaattgg ttgaagctgt agctgaaact gatgaagaac ttactttgaa atacttagaa
    43081 ggtgaagaaa tcactgaagc tgagttaaaa gaaggtatcc gccgcgcaac agttaacgta
    43141 gaattctacc cagtactatg tggttcagca tttaagaaca aaggggttca attactattg
    43201 gacgctgttc ttgattactt gccatcaccg ttagatatcc cagctatcaa agggattgac
    43261 cctaaaactg atgaagaagt agaacgtcct gcagacgaca gcgctccttt ctcagcttta
    43321 gcatttaaag ttatgactga cccattcgta ggtcgtttaa ctttcttccg tgtgtactct
    43381 ggtgttttac aatcaggttc atacgtacaa aacgcaacaa aaggcaaacg tgaacgtgtt
    43441 ggtcgtatcc tacagatgca tgccaactct cgttcagaaa tctctgaagt ttacgctgga
    43501 gatatcgctg ctgctgttgg tttgaaagac acaactactg gggacacact atgtgatgaa
    43561 aagagtctag ttattcttga atccatggaa ttcccagaac cagttatcca agtcgctatt
    43621 gaacctaaat caaaagctga ccaagataaa atgggtgtgg ctttacaaaa actatctgaa
    43681 gaagatccta ctttccgtgc tgaaacaaac gtggaaacag gcgaaacaat catcgctggt
    43741 atgggtgaac ttcacttaga tatcatcgtt gaccgtatgc gtcgtgaatt caaagttgaa
    43801 gctaacgtag gtgcgcctca agtatcttac cgtgaaactt tccgtgctgg cactcaagcc
    43861 gaaggtaagt ttgtacgtca gtctggtggt aaaggtcaat acggtcacgt atggattgaa
    43921 tttacaccaa atgaagaagg tgctggtttc gaatttgaaa atgcgatcgt cggtggggtt
    43981 gttcctcgtg aatacattcc agctgttgaa actggtttga aagatgctat ggaaaatggt
    44041 gtcttagctg gctatccatt agtagatatc aaagctaaac tttacgatgg ttcataccat
    44101 gatgtcgatt caaatgaaac tgccttccgt gttgctgctt ctatggcatt acgtgcagca
    44161 gctaagaaag ctaatccagt aatccttgaa ccaatcatgg ctgtagaagt tgttatccct
    44221 gaagattatt taggtgatgt tatgggtcac gtaactgctc gtcgtggacg tgttgaaggt
    44281 atggaagctc gcgctggcgg ccaacaagtt gttcgtgcga tggttccact tgctgaaatg
    44341 tttggttatg ctacaactct tcgttctgct actcaaggtc gtggtacatt tacaatgaca
    44401 tttgaccact acgaagatgt accaaaatca gtacaagaag aaatcattaa gaaaaacggc
    44461 ggaaaagctg aataatttct taaaaccgga tagtcctgca aagagattat ccaaagagac
    44521 tatattttta atatagtttc gtgtaaaata attatcgatg atatgggcat gtggtgtacc
    44581 ttgtgtacat catataccta tcagacaaat aaaaaatata ttgtattttt aggaggacat
    44641 tttaaaatgg caaaagaaaa atttgaccgt tctaaaccac acgttaacat tggtactatc
    44701 ggacacgttg accatggtaa aactacatta acagctgcaa tcacaactgt actatctaag
    44761 aaaaacggcg gccaagctat ggcttacgat caaatcgatg gtgctccaga agaacgcgaa
    44821 cgcggaatca caatctcaac agctcacgtt gagtatgaaa cagatacacg ccactacgct
    44881 cacgttgact gcccaggaca cgcggattac gttaaaaaca tgatcactgg tgctgctcaa
    44941 atggacggag ctatcttggt agtttctgct gctgacggcc caatgcctca aactcgtgaa
    45001 cacatcctat tgtctcgtca agttggtgtt ccttacatcg ttgtattctt gaacaaagta
    45061 gacatggttg atgacgaaga attactagaa ttagttgaaa tggaagttcg tgacctatta
    45121 acagaatacg aattccctgg tgacgatgtt cctgtagttg ctggatcagc tttgaaagct
    45181 ctagaaggcg acgcttcata cgaagaaaaa attcttgaat taatggctgc agttgacgaa
    45241 tacatcccaa ctccagaacg tgacaacgac aaaccattca tgatgccagt tgaagacgtg
    45301 ttctcaatta ctggacgtgg tactgttgct acaggtcgtg ttgaacgtgg acaagttcgc
    45361 gttggtgacg aagttgaagt tgttggtatt gctgaagaaa cttcaaaaac aacagttact
    45421 ggtgttgaaa tgttccgtaa attgttagac tacgctgaag ctggagacaa cattggtgct
    45481 ttactacgtg gtgttgcacg tgaagacatc caacgtggac aagttttagc taaaccaggt
    45541 acaatcacac ctcatacaaa attctctgca gaagtatacg tgttgacaaa agaagaaggt
    45601 ggacgtcata ctccattctt cactaactac cgtccacaat tctacttccg tacaactgac
    45661 gtaacaggtg ttgttgaatt accagaagga actgaaatgg ttatgcctgg cgacaacgta
    45721 acaatggaag ttgaattaat ccacccaatc gctatcgaaa acggtactaa attctcaatc
    45781 cgtgaaggcg gacgtacagt tggtgccggt gtcgttacag aaatcaaagc ttaattcact
    45841 taagttttaa agtaaagcaa catttattcg gacgtaatcg ctcggaacga aggtcttagg
    45901 atctttgttc cgagcttttt tatttattgc aggatcagga aaaaagacag gagtattata
    45961 aatagaagaa acacgaaata aaagagtcag tattttctaa atgaaacttt atgaaaacaa
    46021 aatgtttttt acaagaaaaa gccttgcttt gtgagtttat atatattata atgacaaagg
    46081 tgcgtttact ttagtgaatt gcacagacgt aatcagaatg atttcgtcgg ctaagaaacg
    46141 agaggttgcg acacgcccgg acgctttgcc acgaacgagc gtgacggaaa ttttcgtgga
    46201 gctatgtcta cttttcaaaa taggcgaagg agggaacaag atggcaaaac aaaaaattcg
    46261 tatccgttta aaagcgtatg aacaccgtat tttagatcaa tcagcggata aaatcgtgga
    46321 aactgcaaaa agaactggag ctagcgtgtc tggtccaatt ccattaccaa ctgaacgcag
    46381 tctttacaca atcatccgtg cgactcataa atacaaagat tcacgcgaac aattcgaaat
    46441 gcgtacacac aaacgtctta tcgacattgt gaacccaaca ccaaaaactg ttgatgcttt
    46501 gatgaagcta gacttaccat ctggtgtaaa catcgaaatc aaattataaa actaaaacaa
    46561 aaataatgga ggtgtactca tgaccaaagg aatcttaggg aaaaaagtgg gaatgacaca
    46621 aatcttcact gaatctggtg aattaattcc agtaactgtt gtagaagcta ctccaaacgt
    46681 agtactacaa gtgaaaacaa tggaaacgga tggctacgaa gctattcaag ttggttacca
    46741 agatatgcgt gaagttttat caaacaaacc tgcgaaaggt catgttgcaa aagcaaacac
    46801 ggctcctaag cgcttcattc gtgaattcaa aaatgttgag ctaggagaat atgaagtagg
    46861 aaaagaaatt acagtagatg ttttccaagc aggagacatt attgatgtaa caggtactac
    46921 gaaaggtaaa ggattccaag gggttatcaa acgccacgga caaagccgcg gaccaatggc
    46981 tcacggttct cgttaccatc gtcgtcctgg gtcaatgggt ccagttgcac ctaaccgtgt
    47041 atttaaaaac aaacgtcttg ctggccgtat gggtggcaac cgcgtaacta tccaaaactt
    47101 ggaagttgta cgtgtcgatg cagaaagaaa tgttatcttg atcaaaggta atgttcctgg
    47161 agctaaaaaa tctttaatta ctatcaaatc agctgtgaaa gctaaataat tgtaggaaga
    47221 aaggaggaac taaggaatgc cgaatgtagc attattcaaa caagatggaa gccaaaacgg
    47281 tgaaatcaca cttaacgaag aaatcttcgg aattgaacct aatgaaagtg ttgtttacga
    47341 tgcaatcgta atgcaacgtg cgtcattaag acaaggaaca cacgcggtta aaaaccgtag
    47401 cgctgttcgc ggcggcggac gtaaaccatg gcgtcaaaaa ggaacaggtc gtgcacgtca
    47461 aggatctatc cgctcaccac aatggcgtgg aggtggcgta gtctttggac caacaccacg
    47521 ttcttacagc tacaaacttc ctaaaaaagt tcgtcgttta gcaatgaaat ctgtattgtc
    47581 agaaaaagtt gctgaaaaca acttggtagc aatcgaaggc ttgaacttcg acgcaccaaa
    47641 aacaaaagaa ttcaaacaag ttcttgctaa cttatctatc gactctaaag tattggttgt
    47701 tctagaaaaa ggtaacgact ttgctgcttt atcagcacgt aacttaccaa acgtttctgt
    47761 agtaacttct gataatgtga gtgtgctaga cgttgtatca aatacaaaag tgttagcaac
    47821 acaaactgct cttactcaaa ttgaggaggt gcttgcataa tgaacttact agacgtaatc
    47881 aaacgcccag tgatcactga aaaatcaatg cttgccatgg atgacaagaa atacactttt
    47941 gaagtggaca ctcgcgcaaa caaaacttta gtaaaacaag cggtagaagc tgcttttgac
    48001 gttaaagtca aaaacgtgaa catcgtgaac gtacgtgcga aattcaaacg tatgggcaaa
    48061 tatgcaggat atacaaaaaa acgtcgcaaa gcgatcgtta cattgactga agattcaaaa
    48121 gaaatccaat tattcgaagc tgctgaataa gcactaatag ataactcatt aaattaggag
    48181 ggaaaaacgt ggcgattaaa aagtacaaac ctacctcaaa tggtcgtcgt aacatgacag
    48241 gttctgattt tgctgaaatc acaacatcaa cacctgaaaa atcattgtta caaccattga
    48301 aaaaccatgc tggacgtaac aacaacggcc gcatcactgt acgtcatcaa ggtggcggac
    48361 acaaacgcca atatcgcgtg atcgacttca aacgtaacaa agataacgtt gttgcaactg
    48421 ttcaaacgat cgaatatgat ccaaaccgtt cagctaacat cgcgttagta cactatgaag
    48481 atggagtgaa agcttacatc ttagcaccta aaggactaga agtgggtatg aaacttgttt
    48541 caggtccaga agcagatatc aaagtcggaa atgctcttcc attagaaaac attccagttg
    48601 gtactgttat ccacaacatt gaaatgaaac ctggtaaagg tggacaattg atccgttctg
    48661 ctggtacaag tgctcaagta cttggtaaag aaggcaaata cgtattgatc cgcttgaact
    48721 ctggtgaagt tcgtatgatc ttagctgcat gccgtgctac aatcggttct gtaggtaacg
    48781 aacaacacga attaatcaac atcggtaaag ctggacgctc tcgttggatg cgtaaacgcc
    48841 caactgtacg tggtagcgta atgaacccta atgatcaccc acacggtggt ggtgaaggta
    48901 aagctcctat cggacgtaaa gcaccagttt ctccttgggg tcaaccagct cttggttaca
    48961 aaactcgtaa caaaaaagct aaatcagaca aacttattgt tcgtcgtcgc aaaaataaat
    49021 aattactttg accatgtgtc gcacattttg agaggaggtt caccatgggt cgtagtttaa
    49081 agaaaggacc ttttgtcgat gagcatttaa tgaaaaaagt tgaagctcaa gcaggggttg
    49141 aaaagaaaaa agttatcaaa acttggtcac gccgttcaac agttttccca agttttgttg
    49201 gatataccat cgcagtttat gatggacgta aacatgtacc agtttacatc caagaagaca
    49261 tggtaggaca caaattaggt gaatttgcac ctacaagaac ttatcgtgga catgctgctg
    49321 acgataagaa aacgaaacgc taatttgagg ggaggataaa tccaaatggc agaacaaatc
    49381 acatcagcta aagcaactgc aaaaacagtt cgcacttcac ctcgaaaagc ccgtttagta
    49441 attgacctta tcagaggtaa aagtgtcgca gatgcaattt caatcttgaa attcacacca
    49501 aacaaatctg ctggaatcat tgaaaaagta ttgatgtcag cagttgcgaa tgcagaaaat
    49561 aactttgact tagatgttga aaacttggta gtaagcgaag cttttgttaa cgaaggacct
    49621 acaatgaaac gtttccgtcc tcgtgcaaaa ggatcagctt ctccaatcaa caaacgtaca
    49681 agtcatatca cggtagtcgt atcagaaaaa taaggaggga taatcagtgg gtcaaaaagt
    49741 acatccaatt ggaatgcgtg taggcatcat ccgcgactgg gatgctaaat ggtatgctga
    49801 aaaagagtat gctgagttct tacacgaaga tttaagaatc cgtaaattta tttcaactaa
    49861 acttgctgat gctgctgtat caacagttga aatcgaacgc gcaacaggtc gcgtaaacat
    49921 ttcaattcac acagctaaac caggtatggt tattggtaaa ggcggttctg aagtcgaaag
    49981 cctaagaaaa gaattaaaca aattaactgg taaaagaatc catatcaata tcgtggaaat
    50041 caaaaaacca gatctagatg caaaattagt aggtgaagga atcgcacgtc aattagaaaa
    50101 ccgtgttgct ttccgtcgcg ctcaaaaaca agccatccaa cgctcaatgc gtgctggcgc
    50161 tcaagggatc aaaactcaag tatctggtcg tctaaacgga gcagatatcg ctcgttcaga
    50221 aggatactca gaaggaactg ttcctcttca tactttacgt gcggacattg attacgcatg
    50281 ggaagaagca gatacaacat acggaaaact aggagttaaa gtgtggatct atcgtggaga
    50341 aattcttcca actaaaaaaa acactgagaa aggagggaaa taatcatgtt agtacctaaa
    50401 cgtgtaaaac accgtcgtga attccgcgga aaaatgcgcg gggaagctaa aggcggaaaa
    50461 gaagtagcat tcggtgaata cggtttgcaa gctgttgatt cacattggat cacaaaccgc
    50521 caaatcgaag ctgctcgtat cgcaatgact cgttacatga aacgtggtgg gaaagtatgg
    50581 attaaaattt tccctcacaa atcttatact gccaaagcaa ttggggtacg tatgggttct
    50641 ggtaaagggg cacctgaagg atgggttgca ccagtaaaac gtggtaaaat catgtttgaa
    50701 atcgcaggcg ttcctgaaga agtagctcgt gaagcgttac gtctagcttc tcacaaatta
    50761 ccaatgaaaa ctaagatcgt aaaacgtgag gaaatgggtg gtgaatcgaa tgaaggttaa
    50821 agaaatcaga gaattaacca ctgccgaaat gcttgatcaa gaaaaacaat taaaagaaga
    50881 attgtttaat cttagattcc aattagcaac aggtcaatta gaaaacactg cacgtattaa
    50941 agaagtacgt aaatcgattg cacgcatcaa aactgtgttg cgtgaacaag ctaagtaatt
    51001 atggaaggag gccattaagc gtatgactga aggaagaaat caacgcaaag tttatcaagg
    51061 tcgcgtggtt tcagacaaaa tggataaaac aattaccgtt gctgtcgaaa caaagaaaaa
    51121 ccaccctatc tatggcaaac gcatgaacta ttctaagaaa tacaaagtgc atgatgaaaa
    51181 caacacagca aaagttggcg acatcgtgag aattatggaa actcgtccat tatcagctac
    51241 aaaacgtttc cgtttgttgg aagtagtcga agaagcagtt attatctaat aacacgagat
    51301 tttcctatat agattgaatg caaaatctga aaggaggata cacaacgtga tccaagcaga
    51361 aagtcgttta aaagtcgctg ataactcagg cgcacgtgaa attttgacga tcaaagtcct
    51421 tggtggatct ggtcgtaaaa ctgctaacat cggtgatgtg atcgttgcta cggttaaaca
    51481 agcaacgcca ggtggggttg tcaaaaaagg tgacgtcgtt aaagccgtta tcgttcgaac
    51541 aaaatcagga gctcgtcgta cagacggttc atatattaaa tttgatgaaa atgctgcagt
    51601 gattattcgt gatgataaaa gccctcgtgg aacacgtatc tttgggccag ttgcacgtga
    51661 actacgtgaa aacaacttca tgaagatcgt ttcactagca ccagaagttt tataatcttg
    51721 agacacttat caaaggaggt gcgaaacagt tatgtttgtt aaaaaaggcg ataaagttaa
    51781 agttatcact ggaaaagaca aaaacaaaga aggcgttgta ctagcagcgt tcccgaaaaa
    51841 agataaagtt attgtcgaag gtgtaaatgt cgttaaaaaa caccaaaaac cttctcaagc
    51901 tgctcctcaa ggtggaattg ttgagatgga agcgccaatt catgtttcta acgtgatggt
    51961 ggttgactct actggtgtag ctggccgagt tggctacaaa gaagtagacg gcaaaaaagt
    52021 ccgtgtttct aaaaaaaccg gtgaagtttt agataaataa aagtttaagg aaggagggtc
    52081 ttactgaatg aaccgcctaa aagaaaaata tcttaaagaa gtaactccat cattgatgga
    52141 aaaatttaac tatagctctg ttatgcaaac acctaaagtt gaaaaaatcg tcatcaacat
    52201 gggtgttggt gatgcggtat caaacgctaa aaacttagat aaagcagttg aagaattaac
    52261 tttgatcact ggtcaaaaac caatgattac aaaagctaaa aaatcaatcg ctggattccg
    52321 tttacgtgaa ggaatgccaa ttggtgcaaa agttacattg cgcggagaaa gaatgtacga
    52381 attcttagac aaattggttt ctgtttcact accacgtgta cgtgacttcc atggtgtaag
    52441 taaaaaagcc tttgacggtc gtggaaacta tactttaggt attaaagaac aattgatctt
    52501 cccagaagta gattatgatt tagtagataa agtacgtggt atggacatcg ttattgtaac
    52561 aacagcaaac tctgatgaag aatctcgtga gttgttgaca caattaggca tgccattcca
    52621 aaaataatta aaggaggcga actacgtggc taaaaaatca atgattgcta aaaacaaacg
    52681 tcctgcaaaa ttttcaacac aagcttatac tcgttgcgaa cgttgcggac gtccacattc
    52741 agtttatcgt aagttccatc tttgccgtat ttgcttccgc gaacttgcct ataaaggtca
    52801 aattcccggc gtgaagaaag ctagctggta aaaagtaaat tcgcataaga aggaggtaaa
    52861 agatcaatgg tcatgacaga tccaattgca gattttctaa ctcgtatccg taacgcaaac
    52921 atggttaaac atgagtcttt agaagtacct gcatcaaaaa ttaaacgtga catcgctgaa
    52981 atcttgaaac gtgaaggttt cgttcgtgat gttgaataca tcgaagatga caaacaaggc
    53041 gtgatccgtg ttttcctaaa atttggtaaa aacggcgaac gtgttatcac aaacttaaaa
    53101 cgtatctcta aaccaggttt gcgtgcttat gtcaaagctg acgaagtacc aaaagtattg
    53161 aacggtttag gtatcgcaat catttcgact tctgaaggtg ttgtcactga taaagaagct
    53221 cgtgaaaaaa atatcggcgg cgaagttatc gcttacgtat ggtaatcaag tagaatctac
    53281 aaggaggtgt ctttaagtga gccgtatcgg aaataaagtc gttgttatcc ctgaaggagt
    53341 aacagttact caagacggaa acaacgttac agttaaagga ccaaaaggtg aattgactcg
    53401 tactttttca gcagatatca aaatgaatat cgaaggaaac gaagtaactt tcactcgtcc
    53461 aaacgacagt aaagaaatga aaacaattca tggaacaact cgtgcgaact tcaacaacat
    53521 ggttgtaggt gtcagcgaag gtttccaaaa agcgttagaa ctaatcgggg ttgggtaccg
    53581 tgcacaatta caaggatcaa aacttgtatt gaacgttggt tactcacatc cagtagaaat
    53641 cacaccacca gctggagtaa ctgttgaagt accagctaac acacaagtaa ttgttaaagg
    53701 tgcgaacaaa gaagaagttg gcgaattagc tgcaaacatt cgtggaactc gtcctccaga
    53761 accttataaa ggtaaaggta ttcgttatgt tggcgaatat gtacgccgta aagaaggtaa
    53821 aactggtaaa taatcgttta gaaaaattgc atagaagcca gcagcaggaa aaaggcattt
    53881 ggctgaagtt taccttcaaa aaaatacatt tttacttgtg ccgcagcttc tatttttcga
    53941 aacgtattta ctgcttgaaa tagtagtaca ataaaaataa agaggtgaca attgtgatta
    54001 caaaaccaga taagaacaaa acacgtcaaa agagacatcg tcgtgtacgt aacaatatct
    54061 ctggtactgc tgagcgccca cgcttgaacg tttttcgttc taacaaaaac atctacgctc
    54121 aaattattga tgacgtagcg ggtgtaacgc tagcaagtgc ctctaccttg gataaagaaa
    54181 tttcaggtgg aacaaaaaca gaagcagcac aagctgtcgg taaactagtt gctgaacgtg
    54241 cagctgaaaa aggcattaaa gtagtagtct ttgaccgtgg tggatacctt taccatggcc
    54301 gtgtgcaagc tttagctgaa gctgctcgcg aaaatggact agaattttag aagaaggagg
    54361 aataccattc atggtttata tcgatccaaa acatttggaa ttagaagacc gcgttgttgc
    54421 aattaaccgt gtaacaaaag ttgttaaagg tggacgtcgt ctacgttttg ctgctttagt
    54481 tgttgtcggt gacaaaaacg gacacgtagg gtttggtaca ggtaaagctc aagaagttcc
    54541 tgaagcaatc cgtaaagcaa ttgaagatgc gaagaaaaac ttagttgaag tacctatggt
    54601 tggttcaaca atcccacacg aagtaatcgg tgcttttagt ggtggtagaa tcctaatgaa
    54661 acctgctgta gaaggttctg gggttgctgc tggtggacca gttcgtgccg tattagaatt
    54721 agctggggta gcagatatca cttctaaatc tttaggctca aatacaccaa tcaacgttgt
    54781 tcgcgcaaca gttgaaggtt tgaaacaatt gaaacgtgca gaagaagttg cagaacttcg
    54841 cggcaaatca gttgaagaat taatcggata aggaggacaa caataatggc tgaattaaaa
    54901 gttactttaa aacgcagtat tatcggacgt cctcaaaacc aacgcgatac agttaaagcg
    54961 ttaggtctta caaagattaa cagttcagtg gttaagcctt ctaatgaagc aattaaaggc
    55021 atgatcaata ctgtttctca cctagtggac gttgaagaaa tttaatttag aaaaatcata
    55081 actagaacat taaggaggtg ccaaacctat gaaacttcat gaattaaaac ctgctgaagg
    55141 ttcacgccaa gtacgtaacc gtgttggtcg tggtacttca tctggtaacg gaaaaactgc
    55201 cggacgcgga caaaaaggac aaaaagctcg ttcaggcggc ggtgtgcgtt taggatttga
    55261 agggggacaa acaccattgt tccgtcgttt accaaaacga ggattcacaa acgtgaaccg
    55321 taaagactat gcagtcatta atcttgatgt attgaaccgc tttgaagacg gagctgaagt
    55381 aactcctgtt gctttagttg aagctggaat cgtgaaaaac gaaaaagctg gaatcaaagt
    55441 attagctaat ggggaactaa acaagaaatt aactgtgaaa gcagcaaaat tctctaaagc
    55501 agctcaagaa gcaattgaag cggctggtgg atcaatcgag gtgatctaat gtttagacta
    55561 ttaaaagatg cttttaaagt aaaagacatt agatcgaaaa tcctatttac tgtatttgtg
    55621 ttgtttgtat ttcgcctagg tgcacatatt actgtaccag gcgtaaatgc tgcttcattg
    55681 caaaaccttt cttctttgcc atttttcaac atgttgaact tggtaagtgg tagtgcgatg
    55741 caaaactttt ccattttctc aatgggggtt tcaccataca ttactgcttc tatcgtcatt
    55801 cagcttcttc aaatggacat cgtgcctcga tttgttgaat ggtcgaaaca aggggaagtt
    55861 ggtcgtaaaa aactgaatca agcgactcga tatctgacat tagtattggc atttatccaa
    55921 tctgtcggta tcactgctgg attcaatcaa tacgctcaat taggtttcgt gaataatcct
    55981 aacgtagcaa cttacgtaac gatcggtttg attctaacag ccggtacgat gtttgttact
    56041 tggctaggag aacagatcac agaaaaaggt atcggaaatg gcgtatcaat gatcattttt
    56101 gcaggtatca tttctagatt gcctgaaagt atcagagaaa tttatgaaga ttactttata
    56161 aatattgatc cttcagacat atggaaatct gctatttttg tggccatttt actagtagcg
    56221 gtcttgttga tcgtgacatt cgtaacattt ttccaacaag ctgaacgcaa gatcccgatc
    56281 caatatacga aacgcgtagc tggcgcgcct acaagcagct atctgccgtt gaaagtaaat
    56341 gcagctggag ttattccagt tatctttgct agttcgttta ttgctacgcc aaatgctgtt
    56401 ttacaagcat tgagcggtag attcggttct gaaaactggt acaaagtgat gatggagatc
    56461 tttaactata atacagcacc tggtgctacg atttatacga ttctgatcgt tgccttcact
    56521 ttcttctacg cctttgttca agtaaaccct gagaaattag cggaaaactt gcaaaagcaa
    56581 ggaagttata taccaagcgt acgccctggt aaggggactg aagagtatgt ctcaagatta
    56641 ctgatgcgtt taagtacagt tggtgcagtc ttccttggtt tagtggcatt gcttccaatc
    56701 attgctcaaa tggtttggga tcttccacaa tcaatcggat taggcggtac aagcttactg
    56761 atcgttatcg gtgtagcttt agaaactgcg aaacagttag aaggcttaat gctgaaacgt
    56821 aaatatgttg gattcattga gtaagaaagc gtgttgaggt ttcccctcaa cacacgttct
    56881 cacacattta ttaggaggga aaacaatgaa cctcatttta atggggctgc caggcgcagg
    56941 taaaggaaca caagctgaaa aaatcattga tgcttatggt attccgcaca tctcaacagg
    57001 agatatgttc cgcgcagcga tgcaaaacga aacagctctt ggcctggaag caaagtctta
    57061 tatggataaa ggtgcattag ttcctgatga agtaacaaat ggaatcgtga aagagcgact
    57121 agctgaacca gatacagaga aaggcttctt attagatggc tttccacgta cgctagatca
    57181 agcaaaagct ctggacgcaa tgctaaaaga tttgaataaa aaaattgatg ctgtcatcga
    57241 tatccatgtt ggtgaagaaa tcttggtcga acgtctagcc ggccgattta tctgcagcaa
    57301 ttgtggagca acttaccata aaattttcaa ccctacaaaa gttgaagaca catgtgatcg
    57361 ttgcggtggg catgaattct atcaaagaga agatgataaa cctgagacgg ttaaaaatcg
    57421 tctggctatc aacatcaaaa atagtgaacc aatcttggct tattataaag aacaaggttt
    57481 gctaaacact attgatggtg ctcgtgaaat cgatgctgta tttgcagatg tacaaaaaat
    57541 cattgaaaaa tagcacgatt gcccgtagcc gacttgcatt atgcagttac tcttgtcaag
    57601 ttcaataaat tatgatagaa tatgtcagtc cgacttctag agaatcctct agatgatcca
    57661 ttttgagaat tttgaggtgg gaactgcagt ttccgccatt ttatcgtgtg aaaatgcgaa
    57721 acaagtacaa ggaggtactg tgcgtggcaa aagaagatat gattgaagtc gaaggtacag
    57781 tcgtcgaaac tttgccgaat gcaatgttta aagttgaact agaaaacgga caccaagttc
    57841 ttgctactgt ttcaggtaaa attcgtatgc attacatccg tatcttacct ggtgacaaag
    57901 taacggttga attgtctcca tacgatctta accgtggccg tattacttat cgctttaaat
    57961 aattgtactc cgtaaaacta caggaggtat tatcatgaaa gtaagaccat cagtaaaacc
    58021 aatgtgcgaa cattgtaaag taattcgtcg taaaggacgc gttatggtga tttgcccagc
    58081 aaatccaaaa cataaacaac gtcaaggata attggaggtg ctataaatat ggctcgtatt
    58141 gcaggagtag atattcctcg tgataaacgt gtagtagttt cacttactta tatctatggt
    58201 attggtaaca ctactgctaa aaagatctta gctgatgctg gtgtatctga agatgttcgc
    58261 gttcgtgaat taacgaatga acaaacagat gctatccgtg cagaagtaga taaattaaaa
    58321 gttgaagggg atcttcgtcg tgaagtgaac ttgaacatca aacgtttgat ggaaatcggt
    58381 tcttaccgtg gaatccgtca tcgccgtgga ttgcctgttc gtggacaaaa cacgaaaaac
    58441 aatgcacgta ctcgtaaagg cccagctcgc gctatcgctg gcaagaaaaa ataattctta
    58501 agtgaaggag gttaaaactt catggcagca aaaaaagtga atcgtaaacg ccgtgtgaag
    58561 aagaatatag aagcaggtat tgcacatatc cattctacat tcaataacac tatcatcatg
    58621 atcactgata ctcacggaaa tgcattagcg tggtcatcag ctggttcatt aggtttcaaa
    58681 ggaagcaaaa aatcaactcc ttttgccgct caaatggcag cagaaactgc tacaaaagca
    58741 gcaatggaac acggattaaa gactgttgaa gtaacagtta aaggacctgg ttctggacgt
    58801 gaagcagcaa ttcgttcatt acaagctaca ggtttagaag tgactgcaat tcgtgacgtg
    58861 actccagttc ctcataatgg tgcccgccct ccaaaacgcc gtcgtgttta atgagtgccg
    58921 ctcattttga gttttatcag taacgtcatt gaacaagaca cttttcgttt tgaaaggggt
    58981 aaagataaga atgattgagt tcgaaaaacc aagaatcgca aaaattgatg aagaaaaaga
    59041 ttatggcaag ttcatcgttg aacctcttga aagagggtat gggactactt tagggaattc
    59101 cctacgtcgt attttattat cttctttgcc aggtgctgcc atcactaata ttcaaatcga
    59161 tggtgtactg catgaattct ctaccatcaa aggtgttaga gaagacgtca ctcaaatcat
    59221 cttgaacatt aaaggcttag cgctgaaaat gtatggccaa gaagaaaaaa cccttgaaat
    59281 cgatattacc ggacctgcta cagtaactgc tggcgatatt atcgttgata gtgatgtaga
    59341 aattcttaac aaagatatgt acatctgtag tgtctctgaa ggagctacgt tccatgctcg
    59401 cttaacagtg aaacctggac gtggttatgt tcaagcagat gaaaataaaa aagaagatat
    59461 gccaattggc gttcttccag tcgattcaat ctacacacct gttcgtcgtg tgaactatca
    59521 agttgagaac acacgtgttg gacaccgtga agattttgat aaattgacga tggagatttg
    59581 gacagatggt tcaattgaac cgttggaagc aatgagtttg tctgcgaaaa tcatgactga
    59641 acatttggat atttttgtga acttaactga tgaagcaaaa aatgctgaaa tcatggtcga
    59701 aaaagaagaa acacaaaaag aaaaaatgtt ggaaatgacg atcgaagagt tagacttgtc
    59761 tgttcgctct tacaattgtt tgaaacgtgc aggaatcaat actgtacaag aacttacaaa
    59821 taaatctgag cctgaaatga tcaaagtccg taacttagga cgtaaatcat tagaagaagt
    59881 taaagcaaaa ttgcacgact taggtttagg tttacgtaaa gaagattaaa accttttacg
    59941 aaggagggaa accaacgtga gttatcgtaa actaggacgc acatctagcc aacgtaaagc
    60001 gatgttgcgt gatttaacaa ctgatttatt aatcaacgaa cgtattgtga ctactgaagc
    60061 tcgtgcgaaa gaagttcgct caactgcaga aaaaatgatc actttaggta aacgcggaga
    60121 tttgcatgca cgtcgtcaag ctgcggcttt cgtacgtaat gaagttgcca gcgtgcgtga
    60181 agaaaatgag gacatcgtca ttgaatcagc tttacaaaaa ctgttcaacg atattgcacc
    60241 tcgttacgca gaacgtcaag gcggatacac acgcattttg aagacagaac caagacgcgg
    60301 tgatggtgca ccaatggttg tcttagaact tgtctaaaat cacactaata acatcacttt
    60361 atagatgatt cttttagagt gttatgatgt tggaagatat ttttccgagt ctagctcagc
    60421 gctgctcctc aagcgaatat cgcttgggga gtaagcactc atcataaagt gtttttttat
    60481 accttcaatt acctgtcatg acagatatgt ttgaaggcat aaaaaaacat tttaagcgag
    60541 aaataattga aaattttgtc tttttttgat agtgttagta ggaactttag gatgaagaag
    60601 gagtaatcat ggagccaatt attgaattag aaaagataaa ttataaatac cagccagatg
    60661 atcttcgccc cgcattgaaa gatgtctcct ttacaattga taaaggtgag tggatcgcta
    60721 ttattggaca caatggttca ggaaaatcga cacttgccaa aacgatcaat ggattgcttc
    60781 tgccagaatc agggatcgtg aaagtgggaa atcaaatatt ggatgaagaa aatatttgga
    60841 cgatccgaca gatggttgga atgatttttc aaaatccaga caatcagttt gttggttcga
    60901 cagtagaaga tgacgttgct tttggtcttg aaaatcaagg gattcccaga gaagaaatgc
    60961 tagtgcgtgt gaaagatgca cttgagaaag ttagaatggc tgagtttgct tcacgcgagc
    61021 ctgctcgttt atcaggaggg caaaaacaac gtgttgccat tgccggcgtg gttgccttac
    61081 gacctgatat cattatttta gatgaagcaa ccagtatgct agatccagaa ggtcgtgaag
    61141 aagtgatctc tacgatcaag aagatcaaag aagaaagtca attgaccgtt atatctatca
    61201 ctcatgatat tgatgaagca gctaatgcca atcggatact agtgatgaga caaggcgaac
    61261 ttgtccgtga aggtacacca aaagaaattt tttctgctgg tcctgaactg atcgacttag
    61321 gtctagatct accatttcct gaaaaactga agagcgcact gaaagaacgt ggggttgatg
    61381 tgcccagtga atatatgaca gaagaaagga tggtggattg gttatggaca tccgttttga
    61441 acaagtagac ttcacttatc agccgaatac accatttgaa caaagagcct tatttgacat
    61501 caatatgacg atcaaagaaa acagttatac tgcattagta gggcataccg gaagcgggaa
    61561 atcaacctta cttcagcatt tgaatgcgct tgttaagcca acaagcggta cagtccatat
    61621 tggagaacga gatattcagc cggatacgga taataagaat ttaaagccca tccgaaaaaa
    61681 agttggcatc gtctttcagt ttccagaagc acagctattc gaagaaacgg tagcaaaaga
    61741 cattgctttc ggtcctaaaa actttggtgt cagcgaagaa gaagcactag tcctagcaaa
    61801 agaaacatta gaacaagttg ggctggatga aagctatttg gaacgttcgc catttgaact
    61861 ttcaggaggg caaatgcgta gagtagcaat cgctggtgtg cttgccatga ggccggaagt
    61921 acttgtattg gatgaaccaa cggcaggact tgatccccag ggcagaaaag aaatgatgga
    61981 gatgttttgg cgtcttcata aggaacagca gatcactatt gtattagtta cgcatttaat
    62041 ggatgatgta gccaattttg cagattatgt ctatgtgttg gaaaagggac gaatcgttaa
    62101 tagcggggag ccgcaagagg tctttcaaaa cattgaatgg ctaaaagaaa aacaactagg
    62161 cgtacctact gccaccgagt ttgcagaaga attaatggct aaagggatga actttgctac
    62221 cttgcctttg acagctgaag aattagcaga cgctatcgtc cattatgctg gaggaaatgc
    62281 ccatgatgaa taaattgata tttggtcgtt atatgcctgg agattccttg atccatcgat
    62341 tggatccccg agctaaatta ttagccagtt tttattttat cggaatcatt tttttagcaa
    62401 ataattggga aacgtacctt ttgttggcat ttttcacatt actttctgtc ttcttgtcaa
    62461 aagtagattt gaaatttttt atccgaggca ttcgcccatt gatctggcta atcctcttca
    62521 ctgtagcttt gcaggtcttg tttacaagtg gcggagaaac ttattggagt tggggtgttt
    62581 tccatatcac tgaatttggt gtgatgaacg gcctctttat tttctgtcga ttcgtgttga
    62641 tcatttttat gtcgactcta ctcacgctta ctacaccgcc tttggaatta tcggatgcga
    62701 ttgagtactt gcttcgtcca ttgaaagccg tacggttccc agtccacgaa atttcattga
    62761 tgctgtcgat tgctcttcgt tttgtgccta cattgatgga cgaaacagaa aagatcatga
    62821 acgctcagcg cgctcgcggt gttgattttg gtgaaggaaa cctagttcag aaaatgaaag
    62881 cagtcattcc tttgctgatt cctttatttg tcagcagctt caaccgtgcg gaagatcttg
    62941 cgactgctat ggaagcaaga ggctacaaag gtggggaagg aagaacgaaa tatcgtgtat
    63001 tgcattggca taatagagac acgatggtta tcattgcttt tgtattatta actgtgctac
    63061 tgatcttttt aagaggataa acgttcagtg ggccgctatc tattcttttc ttgctgatct
    63121 gtttgcaaga aaggatgcaa gcagtgtatg acaagaaata ttagggtctg tggcagacta
    63181 aggtcacagg cctctttctt ttatctgaaa agaaagaacg aaaaaatttt atagaaaaat
    63241 cattattcca gataaccttt taggaacaaa ggagagaaaa acatggtacg ttacaaagca
    63301 atcattgcgt acgacggaac aaattttaat ggattccaaa aacagccaaa tgggcgtact
    63361 gttcaagaag aagtagaaaa aacattacaa aaaatggcaa atggaaaaga aatcacggtc
    63421 tttggttcgg gaaggacaga tgccggagta catgcgatcg ggcaagtaat ccattttgat
    63481 tatccagagg agcgtccgtt agaacgaatg cgtttcgcgc tagataccca gtcaccagag
    63541 gatattgctg taagacaagt agaaattgtt tcagaagact tccacgcaag atatttggtc
    63601 aaagaaaaga cgtatcaatt tcgagtggat atagggaaac cacgcagtcc ttttcgacgc
    63661 cattatgcaa gttattttcc ttacccactt gatttggaaa aaattcagcg ggcgcttcct
    63721 gatttattag ggactcacga tttcacctct ttttgtgcat caggaagctc gatagaggat
    63781 aaaatacgta cgatctacga ggctaagatg gaggtaaatg aaacaaaaga cgaattgttg
    63841 tttacattta ggggaaatgg cttcttgtat aagatgatac ggatcttagt aggaacgctt
    63901 ttgaaaattg gcaatggccg attgcctgaa gatagtatcc cagatattat tgcgaaaaaa
    63961 gatcgtaatg cagcaggacc tacagcacac cctgaaggac tttatttgta tgaagtgaaa
    64021 tatgaataag aggttatgat agaagtggtc agcttcaaga aataagaagg tatttacgaa
    64081 aattacttct caaaattttg caaaatttcg gcttattttc gaagaagcta cttctggaac
    64141 accgtttaag aaagaggtcg tgagcctgtc atttaactct gagtcacaaa gagcaatttc
    64201 aaaaaacagc ttctcatctt tgatgaatat cgtgacttgt ggacgaggcg ttgataagtg
    64261 aatcatcttt ttgttaccta ttttttctgt aaccaaaaaa cgatggaacg ttgttttgat
    64321 tgcccttggt gcggacagtc tgaacaggtt ccatcgtttc tttattttct acgtcttctt
    64381 ctgtgtgtgt ttcgtggttg ttcttctatt gctggatcaa tcgtgccata aaaggggcga
    64441 ttgcggtcaa ggtaccagac ataaagaata aacaatgcaa tacataggac aaaaaggatt
    64501 gcacctatcg taaatccttc aggcaagtaa ctgaaagtga cagtatgttt acctgctgga
    64561 acttttatca tcaagaatgc gtctttgaaa ttgctgacct tgactggttt cccatcaatt
    64621 ttagctttcc atcctttgtc ataaggaata gttgtcacca acatggaatc ttcagcggta
    64681 tttacagtcc cagaagcttt tctgccagtg gcttccattt cgacaccttt ttctttgatc
    64741 tgattcattg cggcttgata ggctacaaca tctagaccaa cgactttagg ctccatgaaa
    64801 cttatttctt tcgtcccata aaaacttgca gtaaatgtca ctgttgtctc ttgaggataa
    64861 tagccaagat catagtattg accagaaatg ttgatttgcg tttttcgcga tgtaccattg
    64921 acagtgatcg tcgcactaga actttctaat tgcgcaaaat tggttgggaa caaacttaag
    64981 taagcttgtg tatttgcagg cacatggact tcccaagtga gctcttttgg ctggttatct
    65041 gctgtttctg agtaagtgac gccagtcgtt gtctttgtga tcgtcgtatt caactgattc
    65101 ttcaaagtag gtgtatagaa tgtaaaatac tgttcattta gaccagacaa tgtattgatc
    65161 aagtttgtct gactggtcaa attatctgtc actggctgct caacatcatt aacggacaac
    65221 ggtgcagtaa atgctagtgg caatgcattg acattctgat acagtgtata gtcgccgctt
    65281 tgaccagctt ggcggaagcc atatttactg aacgtgcttt cgtttttaga aatgttatat
    65341 ttgattcctg taaacccatc catcagcaaa gtattattgt tataacggat attcaaattt
    65401 gtccctcttg aacggaaacc taatttatcc aggtaagagg aagaattacg atttcttata
    65461 gaagaaaaca aactgatacc actgtaacca tagttgatcg aatcgtttgc agatactgga
    65521 tccagatttt ccaagcggta aaacgagtcg gaatcttttt ttgtttcatt tacaagagct
    65581 ttcaagtcag gataaggatc agtatataaa ctgcgggaag cataattcca atcatcaaga
    65641 atgccatgga tcatactgtt cgtattgacc agtgcttcag cagacatgat caataataac
    65701 aatatactca gatgattgaa tgaaagtttt ttatgctgat aaaatcccac agcaaaagca
    65761 taaatcccta agaaaaccac tgtcaataca aaggaagtca tcgtcacata ggtgtagctt
    65821 ccttcaggtt tgatgcccca tgccaccgca aagagagcag ctacgaccaa aatgattcct
    65881 aacaacagcc caccatcgtc ttgttctatc ttttcccatc cttttcctgc aagcaggagc
    65941 actaagaaag agaacagata ggcataacgg aataaaaaca tattcggtgc atgcatacca
    66001 tgccagaaca aattcaatgg aacaagataa aaacttgcga taagtatggc gaaaagactg
    66061 ccgtacaaaa atttattttt caacggtact ttccgactgg caaaataaaa cagacagagg
    66121 actaatggta gcagacctgc gtaaacgaac ggaatcgatc cgtattttgt cgtgtcataa
    66181 acaccaatca tatttttcat gattagatcc agataagaag tcgcttctgt tttcaacgta
    66241 gtgatctgac tcagtgcctc gccatttgac cgaagatcca gcactgctgg caatacaatg
    66301 atcattgaag cgccgcctgc cagtaaagaa gtgataccat aaggaacaat cgttttttta
    66361 tattctgacc agtttgtcaa tagacgggcg ataaaatata agaaagaaaa tacgccaatc
    66421 ataaaaccca tataaaaact ggataaaaac aagagtaaat aactgataaa taaaagtttc
    66481 ggccgtttaa gatccataac acgatgaatc cccaaaatca ctaatggaag ataaacaaat
    66541 gcatccagcc acatgatgat ctccgaatgt gcagtgataa atgagatcaa tgcgtaactg
    66601 acgctaagtg caacatgccc ccagcgagga atcttgaacg tacgtgcaag aaaccaaaaa
    66661 gccaatccgg cacagccgac cttaagcaaa gtcagaacat acagtgcatc tggcatcatc
    66721 tggtttggaa aaaagaacac cagtggcgtg aagatcccgc ctagatagta agaaattaaa
    66781 gataaataat tcaatcctag agaagcattc catgtataaa aaatactttg ctttccaaga
    66841 agaacatttc ggaagctggc atgaaaatta gaaaactgtg aaaaggcatc acttgccaaa
    66901 atacttctgc tgcttccagg atagattccg atacttagat aaacaagtgc taaaatcaaa
    66961 aacggaatca ggaaactagc tgctgcgtag cgtccgtttt ctctaaagaa tgactttaaa
    67021 ttttttttca tatttatttt ccaccaaatt tctatgaagt tgtattcagt ttatcatatc
    67081 cttgtttttc tctgcttaaa aagagtctag ttttcacaaa aaaataaaat ttagaataaa
    67141 taacttcata atttgttcgt ataacaaaaa taaacgcaaa aaaagggtag gaatcaaaag
    67201 tcccaccctt aaaaattttt attaaataaa aaaccgttca ctatctgaac ggtccaagta
    67261 tcattcaatt aaacgcgttc aacttttcct gatttcaaag cacgagttga cacccaaact
    67321 tttttaggtt ttccgtcgac cataacgcga actttttgta agttaggttt aacagtacgt
    67381 tttgttgagt tcatagcgtg tgaacggttg tttccacttt ttgtcttgcg acctgtaaag
    67441 taacaaactt ttgccatggt aatttcctcc tttgccttga tttggagcaa tagaataatt
    67501 cagctcatac cagactaaca tatcatatag aatgggaatt tgcaagtaca ttttcataga
    67561 tacaattgac aattgagaag aataattgta aattaaacaa ggttatacct gaaattttgc
    67621 aaggtttttg agtactttgg gtaactgcct tttatttcaa gttgcgctat gataaaatat
    67681 gatagaagaa aaatgggagt tttttacccg ttaaggaggc tcattcatgg ctgtaaaaat
    67741 tcaaacgtca gctggaacca ttgaaattac caatgatgta attgcaacag ttgttggtgg
    67801 cgccgcgaca gatgtgttcg gtgttgtagg aatggccagc aaaaatcaaa ttaaagataa
    67861 tattactgag attttgcgta aagaaaatta ctcaagagga gtagttgtcc gccaagaaga
    67921 taatggtatt gctgtagatg tttatacgat tatcagctat ggtacgaaaa tctctgaagt
    67981 ttcccgcaac gtccaagaaa aagtgaaata caatctagag gcattattag gcgtaactgc
    68041 caattcagtt aatgtctttg tacaaggtgt tcgtgtactg ccagactagg caggagactg
    68101 gctggtaact cagcagtcga tctgaaggag gattatctag gtgagtgtaa cagaaataag
    68161 tgcaagccag ttccaggaaa tggtccaggc aggtgcgaat cgtctgcaag taaatgcaga
    68221 atacgtcaat tcattaaatg tattccctgt gccagatggc gatacgggaa caaatatgaa
    68281 tctatctatg actagtggag ctaaggcagt agtagattca acctcagaaa aagtaggaga
    68341 attagcagca atgctttcaa aaggattgct gatgggtgcg cgcggaaatt ccggcgttat
    68401 tttgtctcaa ttgtttcgtg gattttctaa gcaaattcca gatgttgtct cattaaatgc
    68461 ttctgatctt gctgcagcct tcactcatgg agttgaaact gcgtataaag cagtaatgaa
    68521 accagtagaa ggaacgattt tgacagtagc tagagaagca gcgaaagctg gagaaaagaa
    68581 agcaaaaagt actgatgatg tcgttgaagt aatgacagct gttgtaaaag gagcaaagcg
    68641 tgctttagct aaaactccag acttgcttcc agtcctaaaa gaagtcggtg tcgtagacag
    68701 tggagggcaa ggactgctgt ttgtttatga gggcttttta agtgcattga atggagaatt
    68761 ccaagcggat gaaacatatg aaccttctcc agcagaaatg gatgatatgg tcaatgcaga
    68821 acatcatcgc agtgtccaag gtcaaatggc gacagaagat atccattttg gttactgtac
    68881 agaaatcatg gtaaaaatcg gagaaggacc tacagtcgat agtacattcg attacgaaga
    68941 attccgtaac tatttagatg gtataggaga ttctttactt gtggtcaatg atgacgagat
    69001 catcaaagtc cacgtgcata cagaaaaccc aggagaagtc ttgaattatg gacaaaaatt
    69061 cggttcatta gtcaaaatca aagtcgacaa catgcgcttg cagcatgaga cgattttgga
    69121 acacgatgaa gaagtgaacg agtttgcgaa aaaagaaata ccacgtatcc cgtatggtat
    69181 cgtagcgatc gctgctggta aaggcgtcca agagctgttt gaaagtttag gcgctaatta
    69241 tgttatcagt ggtggacaaa caatgaatcc aagtacggaa gacattgtta aagcgattca
    69301 agaagtaaac gcagataaag tgattgtctt gccaaacaat aagaacatct tcatggcagc
    69361 tgatcaagca gcagaagtag cagatatccc agtagcagtt gttccttcaa gaacgatttc
    69421 ccaaggattg acagctatgc tttcattcaa tgatcaagct tctttagaag aaaacaaaga
    69481 aacgatgaca gaaatgttgt ctagcgttgt gagcggtcaa gtcactcacg cgatcagaga
    69541 tacctcaatt gacggtgtag agatcaaaga aggagacttt ttgggcatga tcgatggaaa
    69601 aatcctgatc tctaacccag atatcacagc aacagcgtta agtacactag aacaaatgat
    69661 caatgaggac acagagatcg taacgatctt ggcaggtgat gaaggaacag ttgaacaagc
    69721 aaatgaattt gctgagaaac taacagaaaa acatcctgat ttggaaatcg agatccacca
    69781 aggagatcag cctgtctatc cttatctatt ttctgctgaa taatttctgt gtcagaatat
    69841 ctttctaaca cagtattcat tcataaaagg ccttcggtta gtgatcaggt atgatcgctg
    69901 accaagtcct ttttttgtta aaggagggag gaaaaattta tgcgttcttt agatgatcca
    69961 atcactatgc tggcaggagt cggagaaaaa agagcagcta gtcttgccag cttgggcatc
    70021 caaacgattg aagacttact gacttattat ccttttcgct acgaagatat tcaggaaaga
    70081 aatctaaacg agatccagga tcaagaaaag gttactttga aaggaatcgt agtctcgcct
    70141 cctgtgatga atcgatttgg ttataaaaaa agccgattgc aatttcgtat gatgcaagaa
    70201 catgatgtgt tcaacgtttc tttttttaat caaccttatc taaaagaaaa agtcatttta
    70261 tcggaggaaa tcgccgtata cggtaaatgg gatgcaaagc gtaaagcctt gaacgggatg
    70321 aagattttag gttctcaaac aatggatgac ttttcgccga tatatcacgt caacaaatca
    70381 attcgtcaaa cgacattagt tgatctaatc aaaaaagggt ttcaagaata cggcacattg
    70441 attgaagaaa atctgccaaa caaccttgtg gagaaatatc gactgcttga ccgagcttct
    70501 gctgtaagaa gtatgcattt tccaaaagac cacgaagaaa accatcaagc caaacgacgt
    70561 gtcgtttttg aagagttctt tttatttcaa atgcgtcttc aaggattgaa aaaagcagaa
    70621 aaagcagaaa cgaatggttt ggagatcctt tatgatgtcc aaaaattgaa acaatttact
    70681 caaaagctac cttttgaact gacaaatgca caaaaacgtg taacgaatga aatctgtcgg
    70741 gatcttcgca gtccgcagca tatgcagcga cttttgcagg gagatgtagg tagcggaaaa
    70801 acagtggttg ccgcgattgc gttatacgct accgttacag caggtttcca aggtgcgttg
    70861 atggtgccta cagaaatctt ggcacaacaa catatggaaa gcttgcaaca gctgtttgat
    70921 cccaatgaag taaccatcgc attgattacg gggtcaacga aagcaaaaga acgacgtgag
    70981 ctccttgaaa aactagagca aggggaaatt aacgtcgtga tcggcacaca tgcattgatc
    71041 caagatggag tcgaatttca gaatttaggt ctagttatta cggatgagca gcaccgtttt
    71101 ggcgtgaacc agcggaaagt acttcgtgaa aaaggctggc gaccagatgt cttatttatg
    71161 acagcgactc cgattcctag aacattagct attacagcat atggtgagat ggatgtatct
    71221 atcattgatg aacttccagc tggacgtatc ccgatcgaaa cacgctgggt aagaacacct
    71281 caattggaca gtgtactgga ttggacgaaa aaagaactgg caagaggaca tcaaatgtat
    71341 gtcatctgtc cgctgattga agaatcagaa gcattagatg taaaaaatgc agtggaaatc
    71401 tatgaaaaac ttcgtgaact atttgcacca aaatatgaag ttggcttgtt acatggtaaa
    71461 atgaagaatc aggaaaaaga agtaatcatg gaagccttca aagacaacca gatgcagata
    71521 ttagtttcga ctactgtaat tgaagtaggg gtaaacgtgc cgaacgcgac cgtcatgctg
    71581 atcatggatg cggatcgatt tggattggca cagctgcacc aacttcgtgg acgtgttgga
    71641 cgcggaagtg atgcttccta ctgtatctta gttgctaatc ctaaaaatga actaggtgtg
    71701 gaacggatga aaatcatgac agagaccacc aatggttttg tactaagcga aaaagatctg
    71761 gaattacgtg gtcctggtga agtatttggc ttccgtcaat ctggtctgcc tcaatttgcg
    71821 attgctgact tggtgacgga tggaaatgtt ttggaggtag ccagagaaga ggctgcagcc
    71881 ctttggcaaa taaaggattg gcagatgctt cctgagtatc agcctttagc gcaaagcctc
    71941 caaatagaaa atcaggataa tcgttatttc gattagcaga ctgtgctata ctttatactg
    72001 atgacggaaa aaggagtgga aacaatgaaa attgccgtag atgcaatggg cggagatcac
    72061 gcacctcaag caattgtaga aggcgtggct ttagcgcagc aagattttcc agaagtagaa
    72121 ttcttacttt acggaaaaga agcagaaatc aagaaatact taacaaatga aaaaaacatc
    72181 acgattatcc atacagacga aaaaatcaat agtgatgatg agccagtaaa agcaattcga
    72241 cgaaaaaaga ctgcatcaat ggtccttgct gcacaagcag tgaaaaacgg tgaagcagat
    72301 gctgtatttt cagcagggaa tacaggcgcg ctactagcag cagggttgtt catcgtagga
    72361 cgtatcaaaa atatcgaacg ccctggcttg atgtctacct tgccagtagt agggaaagaa
    72421 ggtgccggtt tcgatatgct ggatcttggc gctaacgcag aaaacaaacc agaacatttg
    72481 ttgcaatacg gtattttagg ttctttttat gcacgtaaag tccgaggtat cggacgtcct
    72541 cgtgtcgcac tcttaaacaa cggaacagaa gcaacaaaag gtagtgaagt gacgaaaaaa
    72601 gcctatgaac tccttcagaa tgaaacaagc ttgaatttta ttggaaacgt agaagctcgt
    72661 gagctactca atggtgtggc tgatgtagtg gtcacagatg gatttacagg caacgctgtt
    72721 ttgaaatcta ttgaaggaac agcaatgaat atgatgaatc tattaaaatc tgctattcta
    72781 aatgaaggga tcaaaggaaa aatgggcgct atgcttttaa aagacggctt acgttctttg
    72841 aaagcagaaa tggattattc caaacatggc ggtgccgttt tgtttggctt gaaagcacca
    72901 gtcattaaaa ctcatggagc aacaggcccc gatgctgttc gctatacgat ccgccaaatc
    72961 cacacaatgc ttgagacaga tgtagttgga caattagtcg aacaatttga aaaaagcgaa
    73021 gaatagtatt tggatatctg aagcgatccg ttccattgaa aaaaaggtgg acattcctga
    73081 aaaatgccag taaaattagc catggtgtag gtaattgaca agtgtatgat tcttggaggt
    73141 gaaaaagttg actcgcgaag aagtatttaa caaagtagcg aaaattattt caaatcattt
    73201 tgagatagat accgataaag tgacggatga attgaacatc aaagatgatt taaaagctga
    73261 ttcaattagt attatggaat tcgttttaga gcttgaagat gaattcggta cggaaatttc
    73321 agatgaagac gctgaacaaa ttgaaacagt cggtggcgca gtagattata tcagcagcca
    73381 tcttaaataa tttcaaataa aag
//
LOCUS       DARBET010000006        62947 bp    DNA     linear   BCT 07-NOV-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4027
            SAMEA114569841-rid20112183.denovo.006, whole genome shotgun
            sequence.
ACCESSION   DARBET010000006 DARBET010000000
VERSION     DARBET010000006.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA114569841
            Sequence Read Archive: ERR12203666
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 62947)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 62947)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001972618.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001972618.1
            Genome Coverage       :: 132x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/07/2023 13:18:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,041
            CDSs (total)                      :: 2,975
            Genes (coding)                    :: 2,908
            CDSs (with protein)               :: 2,908
            Genes (RNA)                       :: 66
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 67
            CDSs (without protein)            :: 67
            Pseudo Genes (ambiguous residues) :: 0 of 67
            Pseudo Genes (frameshifted)       :: 26 of 67
            Pseudo Genes (incomplete)         :: 28 of 67
            Pseudo Genes (internal stop)      :: 20 of 67
            Pseudo Genes (multiple problems)  :: 6 of 67
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..62947
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA114569841-rid20112183.denovo.006"
                     /isolate="VRE4027"
                     /db_xref="taxon:1352"
                     /country="Denmark"
     gene            271..591
                     /locus_tag="SCJ86_000492"
     CDS             271..591
                     /locus_tag="SCJ86_000492"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005877273.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux SMR transporter"
                     /protein_id="HEG1168080.1"
                     /translation="MAWMELIIAGILEVFWSTMMKWSDGFSKINYSFYTVIGMIASFY
                     FLSKAIKSLPMSLSYPIWTGIGAVGSVIIGVVLFHDKLNLSTWFFVGMLLISIIGIKI
                     TSGH"
     gene            745..1650
                     /locus_tag="SCJ86_000493"
     CDS             745..1650
                     /locus_tag="SCJ86_000493"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002316458.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HEG1168081.1"
                     /translation="MKSLSVQELSKNFGHKQALSDVSFSLSPGKIVGLVGPNGAGKTT
                     IMKAILGLIHYSDGTICIGENEISPSSHKGLEQVGALIEYPGIYPFLTGYDHLKLFSE
                     TNDVSAIDTIVNQLKMEKYIHKKAKSYSLGMKQKLGIALALLNHPSIVILDEPMNGLD
                     PQATRDVRKIITDLATQGTSFLISSHLLSELEKMVDELILINQGKIIKQCTMAELNQT
                     AQDFIVISTTNDLAARTILTEAGYTLEDPDKIKLLKENEELLGTVIQLLSAQNIHVTD
                     VQHLTNDLESSLLEILDSVKGEEEK"
     gene            1647..2420
                     /locus_tag="SCJ86_000494"
     CDS             1647..2420
                     /locus_tag="SCJ86_000494"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373993.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HEG1168082.1"
                     /translation="MTLLVKQELFKLIKKKSTAVLSVLLVVLLIGTALLAKKYTTIID
                     PVEMTAQLFSATSWIVFIMIAAASTIISMEAQYGTLKNLLYRKYSRGEILVSKWITLV
                     IYSVYLYLLAIIVTVLMKLILFPSISFTEKVSTGQTLIQSLFTYTLGSYIGLWLILSL
                     VLMIACFINSSGASISAGIVFYFASSIISGILIALIQKWEWIKWNPISMLNLQNQIGN
                     EEIMKQLTHLSTNQMLFGNLAYIVLFLALGYLVFKRKNV"
     gene            complement(2601..4025)
                     /locus_tag="SCJ86_000495"
     CDS             complement(2601..4025)
                     /locus_tag="SCJ86_000495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853071.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HEG1168083.1"
                     /translation="MDTEQQKKGIGLIPLAALVIGSAIGGGVFGIMTDISGSAGGPAI
                     FSWVLVGTAMLMLSLSINNINKKRPDLKGGIFSYAEEGFGRFAGFISGWGYWLTCWLG
                     NVAFATLLMSALGYFFPVFEGGQNLASIVMSTVFLWGYAWLVNRGVENASFVNAIVTF
                     CKLVPLFLFIVVAISTFSIQQFIDNFSSDIILNANGQPIPFSSQVLSCIMVMLWVFVG
                     IEGASVVGSRAKKQSDVGKATILGIFGLIIIYVLVSMLPYGTMTMNQLQTIKAQPAMG
                     YVFEMMVGKWGAVVINGGLIISLVGVWLSWTILPIETMGNMAEDGLLPKAWGKLNKNG
                     APTYAIVLTTLCTNVFLLSLLFTDSAYNFAYTLGTAAIFFTWLFLGLYQMKLSYQRKE
                     WVQFLVGLLASAFQVWAMLVVAFNEVMLVLVLFLPGIYFYLKARKEQNEPVQHIHLDK
                     ILLIGVTMVGVIAMVLFATGMLHV"
     gene            4236..4682
                     /gene="nrdI"
                     /locus_tag="SCJ86_000496"
     CDS             4236..4682
                     /gene="nrdI"
                     /locus_tag="SCJ86_000496"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299965.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class Ib ribonucleoside-diphosphate reductase
                     assembly flavoprotein NrdI"
                     /protein_id="HEG1168084.1"
                     /translation="MNILYISISGNTRAFAKHLAEYAEKMHAEDPVNPEVTLKEIHEN
                     SDFTKETEPFFTFVPTYLDGGNGLDNGDTEILTETMREYLEHEDNHKLCLGVVGSGNK
                     NFNNQYCLTAKQYAQTFGFPFLADYELRGTPSDVTRIYQILAENNK"
     gene            complement(4747..6729)
                     /locus_tag="SCJ86_000497"
     CDS             complement(4747..6729)
                     /locus_tag="SCJ86_000497"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334912.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucose transporter subunit IIA"
                     /protein_id="HEG1168085.1"
                     /translation="MAKNELTLEELAKQIYKGAGGMGNVESIVHCMTRVRMKIINDDL
                     VNVSELKAIPGVLGVVEDEQLQVIIGPGKVNKVAQQMVDLAGVKLGEKLSSVSTATAN
                     SSASGKDRVNERAQEMKAAQKSKQKPSKIKSVLKDISNIFVPMIPAFVGTGIVAGIAA
                     VLANLVTAGTLDAATWQQYIDVMNILKNGMFSYLVIYTGINAAQVFGATPTLGGVIGA
                     VVMLTGMNPEAPLKNLFTGEALAAGQGGILGVIFAVWLLSIVEKKLHKVVPDAVDIIV
                     TPTLSLIAIGLIEIFFIMPLAGFISDGMVGGINWVLGVGGAFAGFVLGTLFLPMVMFG
                     LHQILTPIHVQMIDETGRTLLLPILAMAGAGQVGAALALWIRCKKDKELTEMIKGALP
                     VGILGIGEPLIYGVTLPLGRPFITACIGGGIGGAVIGMIGNVGAIAIGPSGAALIPLI
                     SDGKWLGYILGLLAAYAGGFVATFFFGIPKDRLEKEELATETATVNEVPEKVVPQTSA
                     ESVTILSSVADGTVVSLDKASDPVFAQKMMGEGYFVEPENGHIYSPVTGVVSSVFPTK
                     HAIGITTPNGLEILLHMGINTVDLGGTPFDVKVTEGQQVTPDTLVAEADLEAIKAAGK
                     ETSMIVLVTNMDRVKNFVLEKTGKAKAKAPVMDVKA"
     gene            complement(6743..7639)
                     /gene="murQ"
                     /locus_tag="SCJ86_000498"
     CDS             complement(6743..7639)
                     /gene="murQ"
                     /locus_tag="SCJ86_000498"
                     /EC_number="4.2.1.126"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002368658.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramic acid 6-phosphate etherase"
                     /protein_id="HEG1168086.1"
                     /translation="MSKINLENLTTERRNQETFGLDEMSISQAVQLMNKEDQKVAAAV
                     EKELTSIEPVIANTIESFKKGGRLIYMGAGTSGRLGVLDAAECVPTFGVEPEMVIGLI
                     AGGEEAMTVAVEGAEDSYELGQEDLIKLNLTKNDIVIGIAASGRTPYVIGGLDYAKSI
                     GAVTGTISCNKQAEISKHADFPIEVDAGPEFLTGSTRLKSGTAQKLILNMISTISMIG
                     IGKVFNNLMVDVKPTNEKLIERSKRIIMQATEADYETAEKYFEEADQNVKLAIVMILT
                     NSDKEEAQAKLAEANGFVKGTL"
     gene            complement(7642..8703)
                     /locus_tag="SCJ86_000499"
     CDS             complement(7642..8703)
                     /locus_tag="SCJ86_000499"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002368660.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF871 domain-containing protein"
                     /protein_id="HEG1168087.1"
                     /translation="MFGFSIFMNDSLSEEKKQYIRQMASNGFVGIFTSMHIPEDDVSA
                     YQKRLIELGSEAKKNKLELMVDISGEALERAGFSLEDLTPLKAIGVTGLRMDYHISNQ
                     QIADWSHQLKISLNASTITPKDIDELKEAEADFSQIEAWHNYYPRPETGLDKEWYQKK
                     NQWLKIQGLLVQGFVPGDTELRGPLYQGLPTLEEHRGIHPLAGALDLLASNTDIVYIG
                     DAGLSENVQEQFASFQKEQTVLLHTEPVDEEFYEYILGKHTNRQDDARDVIRSVDARF
                     REIPPIPARNTATRMKGSITLDNEKYLRYMGEIQLTKYDLPADEKVNVVAKVIKEELP
                     LINQIKAGMNYQFIRKEGR"
     gene            complement(8914..9480)
                     /locus_tag="SCJ86_000500"
     CDS             complement(8914..9480)
                     /locus_tag="SCJ86_000500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine phosphatase family protein"
                     /protein_id="HEG1168088.1"
                     /translation="MVKGNFANEGENHMLYVTRHGETTWNAQGLVCGRADVPLTEKGQ
                     MQAQKLAEKVVDLPVPITKIIHSPLQRARDTAQAVADRLSLPLTVDERLVEMDFGDYD
                     GLPSKDENFQKARLAFAVRFPNGESVLDVYARIVPLLKECIEDEENVYLLVCHNALIR
                     VINAYFHPMPNEGFFTFMVDNTELVSYE"
     gene            complement(9508..11010)
                     /gene="eat(A)"
                     /locus_tag="SCJ86_000501"
     CDS             complement(9508..11010)
                     /gene="eat(A)"
                     /locus_tag="SCJ86_000501"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F type ribosomal protection protein Eat(A)"
                     /protein_id="HEG1168089.1"
                     /translation="MSKIEIKNLTFGYDSQGTLLFEQANLNFDTQWKLGLIGRNGRGK
                     TTLLNILQNKLPYQGQVIHQQEFAYFPQQTKDKERLTYYVLNDITDFEIWEIERELQL
                     MQTDPEILWREFSTLSGGEKTKVLLALLFVDDTHFPLIDEPTNHLDISGRKQVAAYLK
                     KKKQGFIVVSHDRGFIDEVVDHVLAIEKSQLELYQGNFSIYEEQKKLRDEFEMAQNEK
                     LKKEVSRLKKTAAEKAEWSRSREGDKTKKQVGFIDTESRRVNKGAVGADAARTMKRSK
                     AIVNRMETQISEKEKLLKDIEYIDSLTMNSQASHHKRLLSVEDLQLGYENLLFEPIHF
                     TIEPHQRVAISGPNGAGKSSIIHYLLGAFNGKVIGEKYQPKHLSISYASQNYEDNRGT
                     LAEFAEKNQVDYQAFLNNLRKLGMERDVFHNKIEQMSMGQRKKVELAKSLSQPAELYT
                     WDEPLNYLDVFNQEQLEQLILNVKPAMLLVEHDQTFLDKVSTEIISLERI"
     gene            complement(11268..12389)
                     /locus_tag="SCJ86_000502"
     CDS             complement(11268..12389)
                     /locus_tag="SCJ86_000502"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerate kinase"
                     /protein_id="HEG1168090.1"
                     /translation="MDILAAIDSFKGSASSFELNQAVLNGWEDRKGRKINVPIADGGE
                     NTAEAIYYALRGTWRTIDGMDLLFRQRKISYLLTTWQGKNTAVIESAQALGLDLLDKP
                     TDQTIRLASSYALGELIKDALLQEVEQIIVTLGGSGCSDGGLGLLQSLGASLEGYTEG
                     NPLLSTKKLHFDRRNVLVSKVKLIIAADVTNPYTGQQGAAWQFGRQKGGIDTTLAFLD
                     KQAGKIAQQIKDFWEVDLDDIPGSGAAGGIGGALFLLGAKMVSGFDLVSQITGLEKKI
                     EQADVIITGEGRIDQQTIHGKVPYGVAVLAKKYRKPIIAICGSRDQELEGLSDFLPIV
                     LSIHLGPIGLEEAMEHRQTLEKTKILGQTLSRFFTILPK"
     gene            12515..13456
                     /locus_tag="SCJ86_000503"
     CDS             12515..13456
                     /locus_tag="SCJ86_000503"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-dehydropantoate 2-reductase"
                     /protein_id="HEG1168091.1"
                     /translation="MKIAIAGAGAMGSRIGLMLHQSGNEVLLIDRWPAHIEAIRTNGL
                     IADFNGKEVVAKLPIYSPEEIIESNEHVDLIVALTKANQLDDMFCSIQSIITDNTYVL
                     CLLNGLGHEDVLEKYVPKKNILFGITMWTAGLAGPGKVTLLGDGEIELENLEPEGEAF
                     TKKVVEVFQEANLNPIYSHNVRYSIWRKACVNGTLNGLCTILDCNIAELGAQKAAESM
                     VRTIVSEFASIAAKEGIILDQEEVYQHIASTYDPDNIGLHYPSMYQDLIKNHRLTEID
                     YINGAIWRKGQKYDIATPYCAFLTQLVHAKEGILGAE"
     gene            complement(13606..14502)
                     /locus_tag="SCJ86_000504"
     CDS             complement(13606..14502)
                     /locus_tag="SCJ86_000504"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323722.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oligosaccharide biosynthesis protein Alg14"
                     /protein_id="HEG1168092.1"
                     /translation="MNKNDVLLVAKNKSLYFLPSIRSLDVWSGKDLLQSEFIKSARQQ
                     LANYQLIVFLDYGFDMEMARRVRPFTNAKIVLFFWNHFKAEHEAMLKQAQDEPAIDEI
                     YHFDFLEAKELGLKHNSSFYSKHMNFSSSEPKTDLFFGATNNGRKERAESYKQEFIKR
                     DVTTNYFILPHRGNEQPGYLTYEEYLKKTGESRGILELLREGQQGVTLRTFESMYFQK
                     KLVTDNQAIAHYHFYRPENIFLLRERDLDELPEFLHTPFKPILSELLEFYDAENWAKR
                     FITNDSTIYEQYEYDLSSVENK"
     gene            14771..16111
                     /locus_tag="SCJ86_000505"
     CDS             14771..16111
                     /locus_tag="SCJ86_000505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HEG1168093.1"
                     /translation="MEDKKLKKQLSSRHITMLALGGAIGAGLFKGSGEAIGIAGPSVL
                     IAFLIGGLILYIVMKGLGKIVLSGGDTHHGLSGLIRPYLGAHSADFTDWVYWSMWIIN
                     IIAEAVAAASFLQLWFPHIPAWVFVFMLAVLTTIINLYSVRLFAETEYWLAFAKISVI
                     ILLIIFATYLVGQQMLGTGVFPTLQQLTDHGGFTPHGMKGIISSLLVVIYSYGGSELI
                     AITVSEADDPKRAIPKAIKGVMGRIISFYIIPLFLLLILFPWNTLAGTNVSPFVMVFE
                     KMNIPFAADIVNFVIVLALFSSINSGVYASSRILFFRLKDRKGSSRKLAVLNKHQVPQ
                     RAVLFCTSVLYLGVALSYFVGDKLFGYLAGSLSYTVLLIWIIISAASFVLALRKGTLW
                     DKGISFFALAVLGLIFLGILFTNSIGVTVLTGLLYLFIYFSYQKKNDAFVLTNE"
     gene            16329..16772
                     /locus_tag="SCJ86_000506"
     CDS             16329..16772
                     /locus_tag="SCJ86_000506"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF305 domain-containing protein"
                     /protein_id="HEG1168094.1"
                     /translation="MKKYWKFLLMIGVSTVVMFGMMYLNVYALDHIFFSRTRVFMALM
                     MGAVMAVIMLLFMWKMYENKKINVMILAASLLVFAGSLFMVRSQTTVNDTAWMKAMIP
                     HHSIAILASKRADLKDPEVKKLAEEIIQAQEEEIDKMKKMIKEME"
     gene            complement(17276..18694)
                     /gene="nhaC"
                     /locus_tag="SCJ86_000507"
     CDS             complement(17276..18694)
                     /gene="nhaC"
                     /locus_tag="SCJ86_000507"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323773.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Na+/H+ antiporter NhaC"
                     /protein_id="HEG1168095.1"
                     /translation="MGKKGTPSFLEATLVLMVIVLGIAIGVVGLKVSPNITILAAIGM
                     VMLYAVIKRYPTDWLHEGIINGIKPGIIPIFIFILVGSLIAVWIQAGIIPTLMVIGFK
                     LISVQWFVPSVFVVCAIVGSAVGSAFTVMSTIGIAFFGIGTTLGLNPALVVGAIVSGA
                     VFGDKMSPLSESTNLAAAIVDADLFKHIKHMMWSTVPAFVVSFFLFMILGHTSKAASL
                     SSIREVTDVLEANFTISIWSLIPLFLMLLCAWKKVPAILTILLNIAVAVGMIFIQNPQ
                     TSLTKLAGVIESGFVSNTGNQQIDSLLSRGGIESMMPTVSLIILTLSLGGLLIEFGLI
                     STVMERVSRKMTNTPNLIFTTLMTSIGVNIFIGEQFLSVILPGNAFKETYEKAGFDPT
                     VLGRTLEDGGTVINYLVPWGIAGSFVASTFGIPTLTYLPFVFFSLLSPVFSMVSAFTG
                     WGISKKFVKPQEAVSKTAQLEQ"
     gene            complement(19097..20320)
                     /locus_tag="SCJ86_000508"
     CDS             complement(19097..20320)
                     /locus_tag="SCJ86_000508"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M20 family metallopeptidase"
                     /protein_id="HEG1168096.1"
                     /translation="MTTTISTNQLKDLIQYRLPEYQALALDIHEHPEVSNYEFYSSAA
                     LIKQLEKERFWVEKEVAGHRTAFDARYISNKPGPTIAFLAEFDALPGIGHACGHNLFG
                     TYSVLAASIVKQFIDETGGEIRVYGTPGEEGGENGSAKGSFVREGFFEDVDVALCVHP
                     AFRYGKTTESLANDPVDIKFYGVASHAAAAPEKGVNALEALIQVFNGINALRLQLPKD
                     VNIHGIITDGGVAANVIPDYAAGRFYLRAGNRQTLDKVYEKVENIVRGAAISTGTTFE
                     FGLFQNAVDDVIVTPSFDDLFFQHAKEAGVPDEEIETEQRTSLGSSDVGNVSQVIPTI
                     QPTVAISETYIAGHTEEFKAAAKSDKGLASIAIAAELLANTALDLLKEPKLLERIKQE
                     HQEIVARKKQEGATF"
     gene            complement(20351..21190)
                     /locus_tag="SCJ86_000509"
     CDS             complement(20351..21190)
                     /locus_tag="SCJ86_000509"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293787.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MetQ/NlpA family ABC transporter
                     substrate-binding protein"
                     /protein_id="HEG1168097.1"
                     /translation="MKKIIRFVVITFVAIIWLAGCSQGKADTTDKKETIVKLGIIGED
                     TDVWDNVKDRLKKENINLEYVKFTDYNQPNVALADGSIDLNSFQHQFFLDNYNKEHGT
                     DLVSIGNTVNAPLGIYSNTLKDVADIKDGAKVAIPNDVTNGGRALLLLQTAGLIKVDK
                     AKGQAPTVSDITENRKNLDISELDASQTARALSDVDISVINSGVAVDAGFNPASDAIF
                     LEPVDDHARPYVNIIVARKEDENNKTYQKIVDAYQTEDTVKVIEKTSKGSSVPAWETF
                     GRK"
     gene            complement(21207..21887)
                     /locus_tag="SCJ86_000510"
     CDS             complement(21207..21887)
                     /locus_tag="SCJ86_000510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346795.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEG1168098.1"
                     /translation="MTDFFKTYLPNVYLIPDEFVEATKQTLYMSFWTAFIGGMIGIIL
                     GVTLVVTRPNGLLANRLLFEILDKLINIIRSIPFIILLSLLALTTRFLVGTTIGAKAA
                     LVPLIFGVVPFFARQIENALLEVDPGVIEAAEAMGTSPIGIILRVYLLEGLPGIIRVS
                     ALTIINVIGLTTMAGAVGAGGLGNLAISRGYNRFQTDVTIVATLLILILVFLSQFISN
                     QLIKKITH"
     gene            complement(21889..22962)
                     /locus_tag="SCJ86_000511"
     CDS             complement(21889..22962)
                     /locus_tag="SCJ86_000511"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HEG1168099.1"
                     /translation="MIELRDVSVCFKQKEKEIQAVDSVDLTIEKGDVYGIVGYSGAGK
                     STLVRVMNLLQKPTAGEVIVNQTNLSQLSPKELRKERKAIGMIFQHFNLMESRTIFDN
                     VDFSLKYSGKSKQERRQKVNELLELVGLEEKASAYPKQLSGGQKQRVAIARALANEPK
                     VLLCDEATSALDPKTTLQILALLKKLNRQLGLTIVLITHEMQVVKEICNKVAVMEDGR
                     IVEKGSSIQIFSNPEEELTKDFIRTATHLDQALETIIAHSAFADQIANKWLVELSYIG
                     NQTNEPLIAHLYSKYQVTANILYGNVELLQETPLGSLIVTLAGETKQRKKALDYLIKS
                     GVKINILQKNDLDSRIKVIEGGR"
     misc_binding    complement(23018..23256)
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00230"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="T-box leader; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="tRNA"
                     /db_xref="RFAM:RF00230"
     misc_binding    complement(23299..23532)
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00230"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="T-box leader; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="tRNA"
                     /db_xref="RFAM:RF00230"
     gene            complement(23609..24280)
                     /locus_tag="SCJ86_000512"
     CDS             complement(23609..24280)
                     /locus_tag="SCJ86_000512"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629846.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family phosphatase"
                     /protein_id="HEG1168100.1"
                     /translation="MKGAIFDLDGLLVDTEKTYRDGWLWGFQQFDIHIPKEIVDAWGG
                     KNWKQSFDILVKAAGSPEKVQEVRAKREEYFYQQLTSGAIHLKPYAKEILADLKERHM
                     KLGIATTTVTKRATDILDHFKLADYLDTFTFGDEVSENKPSPIPYLTALERTGLEASS
                     AFAVEDSLVGATAASRAGLGVVLIPDSSFVRNYTVEEKEKLKLLAEGKDLRTVIEMLN
                     KKTEK"
     gene            complement(24367..27045)
                     /gene="mgtA"
                     /locus_tag="SCJ86_000513"
     CDS             complement(24367..27045)
                     /gene="mgtA"
                     /locus_tag="SCJ86_000513"
                     /EC_number="7.2.2.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313416.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium-translocating P-type ATPase"
                     /protein_id="HEG1168101.1"
                     /translation="MSFGHKGEIEMEKKKDTQKIYAAFAGKTKQELFELFHASANGLT
                     EEQIERSRENYGENAISYGKKTPFIVEILKAYITPFTLVLIALGIISFITEYVLAAPG
                     DKDLFGVAIIFTMVIVSGTMTLIQSVRSNQAAEKLKSLVKVTAAVKRNGKYTEIPMEE
                     IVCGDLVRLSAGDMIPADMRLLSAKDLFISQAALTGESYPVEKHAKVCEDDQQSETSY
                     ENLVFMGSNVISGSAEGIIVSVGNETLFGHVAETLSEKPIQSSFELGIHKTSMLLIKF
                     MSLMAPAVIVINGLTKGDWLEAFLFGLSVAVGLTPEMLPMIVTTNLVKGASVMAKKGT
                     VIKNLNAIQNFGAIDVLCTDKTGTLTQDKIILEYHMDCEGNEDDRVLRHAYLNSYYQT
                     GLKNLLDVAIIDEATETLDTDKINYQKVDEIPFDFERRRMSVVVADPAGKTQMITKGA
                     IEEMLNISKFIDLGGTVTPLTKERKEAVLAKVQSLNEDGLRVIGVAQKTNPSVVGEFS
                     VKDESDMVLIGYLAFLDPPKETTKQALKALKEHGVAVKVLTGDNELVTRSVCRQVGLE
                     INELVTGEKISEMSDHELAQVAEDHEVFVKLNPQQKARLTTALRKNGHTVGFLGDGIN
                     DAPAMKVADIGISVDTAVDIAKESADVILLEKDLTILERGLLSGRETFGNIMKYIKAT
                     ASSNFGNMFSVLIASTFLPFLPMLPLQLLFLNLIYDVSCISLPWDRMDKEYLDEPKKW
                     NASSIGEFMVYFGPTSSIFDITTYLLMYFVICPAVVGGSFHTLDSSQRVIFIALFHAG
                     WFVESLWTQTLVLHALRTPKVPFIQSNATFAMFVITTLGIIVGSVLPFTSFGAELGLM
                     PLPGNYWLWLFATVIAYLALVTAVKKIYIRRFQELL"
     gene            27704..28141
                     /gene="ndk"
                     /locus_tag="SCJ86_000514"
     CDS             27704..28141
                     /gene="ndk"
                     /locus_tag="SCJ86_000514"
                     /EC_number="2.7.4.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside-diphosphate kinase"
                     /protein_id="HEG1168102.1"
                     /translation="MERTLVIIKPDGVRRHLVGSIIQRFEAKGLAIAEMKFETMTPEL
                     AKEHYQHLTERSFFDELIDYMTSGPVVYLVLVGEEVIDIVRKMVGATKAADAVPGTIR
                     GDYALPGTENIIHASDSRDAAVKEIARFFPPFDINKPSEKAAK"
     gene            complement(28163..29029)
                     /locus_tag="SCJ86_000515"
     CDS             complement(28163..29029)
                     /locus_tag="SCJ86_000515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293329.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HEG1168103.1"
                     /translation="MYDGELIKKLRVNKKLTQSQLAEGICFKTSLVGIETNSVKKMSF
                     VTLRSFLERMNMTLAEYEWMRNQLDEPIKLKKKRRMLDRVQEDSFDPYKEISNNRRHF
                     KKTEDLYYLVLNLHMVEKTEVSETEFEFLRYECRIIEEYLMQIPDYGQFELSILADFP
                     WIFSDRFIENNYLKIKKRMRRLAQLNNHEQYLFTFLMNLTSFYIENGRLKKARSVNED
                     MKRSLAHKERSTVIYETLMADYYQRLISAALGEEKTKDYQEFFTVLEYMLGKNQRRSL
                     EKKLLAIAEPSN"
     gene            complement(29189..31282)
                     /locus_tag="SCJ86_000516"
     CDS             complement(29189..31282)
                     /locus_tag="SCJ86_000516"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LTA synthase family protein"
                     /protein_id="HEG1168104.1"
                     /translation="MTQLKKLCQNRLAIVLTAVFFFWLKTIIAYYADFSLGVEGTIQY
                     FILWINPIATTLLFFGLSLYIKKPKPALAILLIIDILNTLLLYLNIIFYREFTDFITV
                     KSVLGFSKVSQGLSGSSFSLMKPHDVIYWLDIAVFIGLLVWLKVKKIPIKSNPVGKPM
                     AIAVTCLSGLIFSGNLALSEANRPQLLQRTFDRSYIVKYLGIDAYTIYDGIKTGMTSS
                     VRAHASSNGIDEVLDYTKKHYAEPNPETFGIAKGKNVIVLHLESFQQFLINMKVDGQE
                     VTPFLNSIFQNQATISFDNFFHEVGQGKTSDAENMLETGTFGLPQGSLFTELGSDNVF
                     QAAPAILGQKQGYTSAVFHGNVASFWNRDHVYKNLGYDNFFDRSYFDESDETLGYGIL
                     DKDLFRESAQYLEHLQQPFYTKFLSVTNHTPYYTDDKNFDFPSLNTGNSTVDNYVRTA
                     HYLDQSLEQFFTYLKKSGIYQNSIFVIYGDHFGISNTDNKDLASALGKDPDTWDEFDN
                     AQMQRVPLMIHMPGYTKGTVNHEYGGEIDVLPTLLHLLGIDDKDYLHFGTDLLSSQHD
                     QVVAFRNGNFVTPKYTVLGGKAYNNQTGEIIDTKAAGIDEEISKDQEKVKTALSLSDK
                     LNQENLLRFYVPNGFETIDPKKYDYKNEAERNEAIEKQKGEHSTSVFSKNGNKTTTNL
                     YPVTRTDVEQQKNNQ"
     gene            complement(32031..32654)
                     /locus_tag="SCJ86_000517"
     CDS             complement(32031..32654)
                     /locus_tag="SCJ86_000517"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299979.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEG1168105.1"
                     /translation="MRKKRPFLLFWLFGFFLLLILSAAFSGKMTADLAQKETEKIVAS
                     KESVDNFKKEFSTYWWAEYKITANYEKGELDIYLPKGKEENQFINIDDVLSAISLRQY
                     QFKDKHLTVLLLTHDGDKVARLKNGKVITAYSEKVDKKVVADRLAEWKKTNEQVKKTT
                     VSSQKNESTGTSPSDSGSGQTITGSEENQASIITNESSQGQYQSYGQ"
     gene            complement(32813..34210)
                     /locus_tag="SCJ86_000518"
     CDS             complement(32813..34210)
                     /locus_tag="SCJ86_000518"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738851.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 1 protein"
                     /protein_id="HEG1168106.1"
                     /translation="MKKYQFPANFWWGSAASGPQTEGRISGDGKGENIFDYWYKKEPQ
                     KFFDQVGPEKTSQVYTKYQEDVQLMKQTGHNSFRTSIQWSRLIPDESGTINEEAVRFY
                     HRYFDELIENGIEPFVNLYHFDMPMYLQEKGGWLNRETVEAYEKYAKSCFELFGSKVK
                     KWFTHNEPIVPVEAGYLYRWHYPEESDMKKAIQVGYHEALASALAIKSYHEMEQGGVI
                     GIILNLTPSYPRDESNPEDVKAAQIADAFFNRSFLDPAVKGEFPSELIEIVKELDLMP
                     VIHAEDLTVIKENTVDLLGINYYQPRRVKAKETPIDTKNGPMPEDYFDNYEMPGRKMN
                     PYRGWEIYEQGIYDILINVRDHYGNIDCFISENGMGVENEARFIKEDGMIHDDYRIEF
                     VQSHLKYVHQAIQEGANCLGYHMWTCMDNWSWTNAYKNRYGFISVDLAQDGKRTIKKS
                     GHWFKDVSDKNGFEA"
     gene            complement(34242..35213)
                     /locus_tag="SCJ86_000519"
     CDS             complement(34242..35213)
                     /locus_tag="SCJ86_000519"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HEG1168107.1"
                     /translation="MASIRDVAKMAGVSVGTVSRYLNGQQLKEKNMKKIAEAISALDY
                     KENIIAKGLKNNRSFSIGLLINGISSRFGSEVVSGIERVAEENGYSLLLSGFADQPEK
                     IEQKIEYLMKHVIDGLIVFLSEEEWSGFEMLTKMSIPVIALNCPEGPAGIDTILVNDR
                     ESVAKVIAHHGEQGHKKIGFIAATQTDYVARERLAGAKEAVQNDPSIQLEVFTGDYSR
                     KSGYYGAKALLEKGATAIFVSNYNMSIGAIECFNQEGVRIGKDIAFSHYDYLDKNDLS
                     ILPKITITPPTDKIGERAAEQLLKRIKKEEEENEKVIVMENIIHGIN"
     gene            35587..36012
                     /locus_tag="SCJ86_000520"
     CDS             35587..36012
                     /locus_tag="SCJ86_000520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373688.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEG1168108.1"
                     /translation="MKKLIVLFILSAGLLTLTGCDLFGGSDQSSDETTDSSVLKAEKA
                     DTTNSVYTLKVRKDMDLSAGQLTFTDKKMEWTRTYQTQGTDSSESSEEKTVLTDIKIT
                     TKDDEYIISGKENKKETTVIFTKIGNYRLKDEDGNIYSL"
     gene            complement(36500..37201)
                     /locus_tag="SCJ86_000521"
     CDS             complement(36500..37201)
                     /locus_tag="SCJ86_000521"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336017.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="deoxynucleoside kinase"
                     /protein_id="HEG1168109.1"
                     /translation="MSVILISGTIGAGKSSLTDMLAKEIDSKPFYENVEDNEVLPLFY
                     SNPEQYAFLLQIFFLNKRFLAMKNALVNDDNVLDRSIYEDSLLFHLNADLGRVTDIEV
                     QQYDSLLDTMLNELDDVAPKKRPDLMVHIKVSLDTMLERIKKRGRDYEQLESDETLYT
                     YYETLNTRYNQWYEDFDVCPKIQVDGDKFDFVENPEDAKKVIRQIQEKLAELEGKPAG
                     KYFTDNQHAKGQELG"
     gene            complement(37217..37657)
                     /locus_tag="SCJ86_000522"
     CDS             complement(37217..37657)
                     /locus_tag="SCJ86_000522"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318723.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside deoxyribosyltransferase"
                     /protein_id="HEG1168110.1"
                     /translation="MSQIYLAGPFFSEEQIDRVSRIEKALEENKTVTSFYSPRHHQES
                     NYELFSAGWAQEVYEKDMEELTNAEFVVAILDFEHQTIDPGTAYELGVATMLKKPMII
                     VQEETVPTNLMITQSLHTYLKSDQAVREYDFETLPVETYVGEYL"
     gene            complement(37657..38232)
                     /locus_tag="SCJ86_000523"
     CDS             complement(37657..38232)
                     /locus_tag="SCJ86_000523"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333798.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR00730 family Rossman fold protein"
                     /protein_id="HEG1168111.1"
                     /translation="MKLTVFCGSRFGNKESYKFIAQTLGKYMAQEEIELVYGGSYSGI
                     MGVISKTVLENNGKVTGIYPNGLFETELPGKDVTTFIPTETIDERKVLLFEKGDAVLV
                     FPGGLGTLEEFSQLLSWIAIGLTPDKPIGILNIGGYYSGLQQLLETFAKEDFMDKKWL
                     DQVIFSNNPLELVNELRAVSIKNQLLLKEAK"
     gene            complement(39080..39559)
                     /locus_tag="SCJ86_000524"
                     /pseudo
     CDS             complement(39080..39559)
                     /locus_tag="SCJ86_000524"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339057.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="PTS fructose transporter subunit IIC"
     gene            complement(39718..40419)
                     /locus_tag="SCJ86_000525"
     CDS             complement(39718..40419)
                     /locus_tag="SCJ86_000525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291138.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HEG1168112.1"
                     /translation="MKRTRVLYLEVADKIKEDIFSGKYPVGSMLPTESELEELFQVSK
                     ITVRKAIELLASDEYVAKKSGRGTTVLSNRPYNRLSKAASFTQILENSRYEVRKETLD
                     LQLVHIKKEDPLFSFFGEQAVCFKRLYYLNNQPYIYFVHYLPQEMMQFSREEFGKESL
                     YRLLTKKGYTIDSFSDDFSAVFLTEEEQGILKTDAQIGIKRIRKSSTQIGEMIEYSEA
                     VYHTELHPYHIDYET"
     gene            complement(40527..41936)
                     /locus_tag="SCJ86_000526"
     CDS             complement(40527..41936)
                     /locus_tag="SCJ86_000526"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016632157.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="family 1 glycosylhydrolase"
                     /protein_id="HEG1168113.1"
                     /translation="MEQLLPNQLEGAYDRDGKGLSVADAMPGGKQRFAIIGSEEFDWT
                     IDQEKYIYPNHRGIDHYDRFKEDLALFAEMGFKCYRFSIAWTRIFPNGDEGTPNEAGL
                     EFYDQLIDECLKYDIEPVITISHYEMPLHLAKEYGGWKNRKLIDFYERFAQTVLERYS
                     SKVKYWMTFNEINSAFHFPALSQGLVKSNGAGEYQNIFQAWHNQFVASSKAVKIGHEL
                     RSDIQIGCMIIYATTYSIDANPVNQAATMIQNQEFNFFCTDVQVRGEYPAYTARTHKK
                     YGVDPEKLEQTEKDFQLLKEYPVDYIGFSYYMSTAINETDPQAATSEGNLLGGVKNPF
                     LEASEWGWQIDPEGLRIALNELYNRYQKPLFIVENGLGAIDHMEEDGTIIDDYRIDYL
                     RRHIEAMANAVADGVDLMGYTPWGCIDLVSASTGEMSKRYGFIYVDLDDEGNGTLNRS
                     KKKSFDWYKEVIASNGVNL"
     gene            complement(41984..43849)
                     /locus_tag="SCJ86_000527"
     CDS             complement(41984..43849)
                     /locus_tag="SCJ86_000527"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330035.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucose transporter subunit IIA"
                     /protein_id="HEG1168114.1"
                     /translation="MDNQAVGRRVWEAVGGQKNVKSLVHCATRLRFRLKDESLADTQK
                     LKEDPDVIQVVQSGGQYQVVIGSNVADVYQSIVDEEGLAVSEDGKEESKNILNRLIDI
                     ISSIFTPFLGAMAAAGVLKGFLSLATVMGWLTADSGTYQILFAAADGVFTFLPVMLAF
                     TAAKKFKANQFLAVAIAMALVYPAITAVAGAGEAISFFGIPVILSPSGYTSSVIPIIL
                     AVWVQSKLEPFVKKVIPQFLQMILVPLVVLVVMVPLTFLALGPIGTVAGNALGGLFNS
                     IYGFSPIVAGLIMGSLWQVFVMFGMHWGFVPIMFLNIEQYGFDVLMPMLLPAILAQGG
                     AALAVALRTKDTKLRALGISSTVTSLFGITEPTVYGVTLPLKKPFIAACISGGIGGAI
                     IGFSGVKAFSSSLVSLLTIPTFINTVDGVESNVTVAVIATGIAFVLAFVGTLILGFDE
                     QTQDNQLENKHANAGKPITSARHTLKSPLTGKVLPLSEVPDQVFSSGVMGKGIAIDPE
                     VGELVAPADGEITTIFPTGHAVGITTTDGAEILIHIGMDTVELNGNGFEILVKQGDLV
                     KAGDLLIRFDIEAIRAAGYSVITPVVITNTDAFADILELDQKEIIANEDVLAIVK"
     gene            complement(43970..44821)
                     /locus_tag="SCJ86_000528"
     CDS             complement(43970..44821)
                     /locus_tag="SCJ86_000528"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PRD domain-containing protein"
                     /protein_id="HEG1168115.1"
                     /translation="MKIDKILNNNVVISKNGFGEEVVCMGKGLAFQKKIGDEISPEAV
                     QKEFVLRDSFAKNQFQQLMADIPAEEIEWVKQVVELAEDKLKVEFSPNIYLTLTDHLH
                     YAITRAKENIQLPNPLLFETKKFYPKEYQAAKEALLFVKQKTGVELSEDEAGFIAFHF
                     VNSQQGNEDMQVTMTATTMVRDILSIISKFFGIVFDEESLNYQRIITHLQFFAQRYLK
                     GEGSDEQDEFLYALVQGKYPKAFHCVERINEYLRQTQKQEMGIAEQIYLTIHIQRVVS
                     EKKSIQS"
     gene            complement(45131..45844)
                     /locus_tag="SCJ86_000529"
     CDS             complement(45131..45844)
                     /locus_tag="SCJ86_000529"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346126.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class A sortase"
                     /protein_id="HEG1168116.1"
                     /translation="MKKKGKAKRWLVNILLFLLLLVGLALIFNEQIKDYFVRETGDKY
                     AIANVTKEDLKKNNDKDVSFDFDAVEPMTTEGVMRSQMRGTDLPVIASIAVPSVSINL
                     PVFKGLDNTSLLYGAGTLSPDQEMGKGNYALASHRATNPELLFTPLENLEMGAKIYLT
                     DLENVYTYKTFFKEKVAPTDTQLLNEVEGKEIVTLITCGDMDAVTRLVVQGELESVTS
                     IKDATDDMRSAFNLETKTF"
     gene            complement(45912..46238)
                     /locus_tag="SCJ86_000530"
     CDS             complement(45912..46238)
                     /locus_tag="SCJ86_000530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotide pyrophosphohydrolase"
                     /protein_id="HEG1168117.1"
                     /translation="MNSMEKVNQFRDERNWRQFHNEKDLAISISLEASELLELFQWKT
                     PEEVKETQLERIKEELADVLIYSYMMADNLHLDLDEIIEEKLIKNNLKYPVEKSAGVR
                     KKYTEL"
     gene            complement(46361..46588)
                     /locus_tag="SCJ86_000531"
     CDS             complement(46361..46588)
                     /locus_tag="SCJ86_000531"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003246265.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="winged helix-turn-helix transcriptional
                     regulator"
                     /protein_id="HEG1168118.1"
                     /translation="MLTQQLRELEQDESIHRKAYNQVPPKVEYSLTEEGKSLREILVA
                     LSVWEEERIEKQKASREEDTLLHTHGGYLNY"
     gene            complement(46910..48163)
                     /locus_tag="SCJ86_000532"
     CDS             complement(46910..48163)
                     /locus_tag="SCJ86_000532"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328519.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GHKL domain-containing protein"
                     /protein_id="HEG1168119.1"
                     /translation="MVATVTLYLTFTLILDLGIYLFFSKQLLVKNSFGCIALMVVTLL
                     LPIPKVSFPYLVGLLLMFLFYFTTKNLFISCLISNLFFAITGLSFFLVFDLMDYLGKR
                     HLGTASFFALLIAFIFYLLVKSIDKQTRFLNLLFTYPTEHYFGGICILLSWGVIYLVL
                     GSVHFRSSNYLLLSLLSIVISFFSLFFSIMLISFQVKERQHQESLQLYQINEEYYHHL
                     EEFKHDYKSLLFSLKITLQENKKETLEFLEILEDHSAAIFEKPQIQQLTNIVSPAVRG
                     VFLKFLEKAESEKIPIQVVIPVEVKTIPIDLVVFIRCLSIFISNAFDHRVADQSPITI
                     NLLEEKEGSRFSIANKANPTNLTLSEMVKRGKTSKKNGGLGLYSAKKMLDVYENAELK
                     IEFSKQNHYFFVEMYLASRKSKSAY"
     gene            48224..48964
                     /locus_tag="SCJ86_000533"
     CDS             48224..48964
                     /locus_tag="SCJ86_000533"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342003.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HEG1168120.1"
                     /translation="MKKYFIIEDNQFHSEWIIRELVNHHIENIVQLTSISQINQLIQL
                     GTIDNHDVFFIDIDLKQSLTGIDVAEQIRKKNAHSNIVFFTAYTNFAVEIINRKILPI
                     GYIRKDRNLSEQIRQVLEQINWKEQQIVNVDKIFIDQKGGSLVLYPREILYIESIKGL
                     KNQVMVKTTTEDKLVNTSLKQLKTELEKYAYFLLLKSYCINLSQLQKIDSAHYVLEFF
                     NGDSLLVGRKIFEKTKERFHDFQKTASS"
     gene            complement(49050..49808)
                     /locus_tag="SCJ86_000534"
     CDS             complement(49050..49808)
                     /locus_tag="SCJ86_000534"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="WxL domain-containing protein"
                     /protein_id="HEG1168121.1"
                     /translation="MKKTIALSTVLAGSLLLCTQSVFAENKSSATTEGTISVTKPDAA
                     NPEDVVPVPPVDPNDPDTPFTPDEPNQGTSGLLTIDQAPDLDFGTIKLGTAKTVYAAL
                     QSGTDSTGAQTQVQNFVQVTDKSGNYAGWKLSVKRTEFIGTTSDTNKLTGSKVKFKNA
                     VIRNSATNSSGMPSTVSGTGAEGIEIPADQAVDLVTAGANEGIGTWIYSLGENVEQGR
                     QSVELFVPVSNYAVDNYVSTFTWSLTDAPTEEEA"
     gene            complement(49805..50833)
                     /locus_tag="SCJ86_000535"
                     /pseudo
     CDS             complement(49805..50833)
                     /locus_tag="SCJ86_000535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628085.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF916 and DUF3324 domain-containing protein"
     gene            complement(50853..54020)
                     /locus_tag="SCJ86_000536"
     CDS             complement(50853..54020)
                     /locus_tag="SCJ86_000536"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333159.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEG1168122.1"
                     /translation="MNQCVKKLIASILFVFPLLMVTSVFADVEKTNESTQSENQSFDK
                     TTFSEESITSETTESQSEASTNKRQEENKTSETSEVAEQTSDSSEAALARAVSADGYS
                     EAATTEELAQLLADESVTKIRLIQPLTLDRELEIKRDIVIDFGGFAHNFGTHHIYINE
                     IPQSIEFQNFKGTAAHPGGLTPNIDGNAIIIAYMTDFWGSRYHFTGEIKFTGTLDLYD
                     GSKLGLIYAPRATVTLDGVSGVLDVQPVKEGLNAAPGKAYFCRSYQLNVINGSQLYGP
                     YLGKFYGFLDSGDETGSGKTAPGIHILSGSKVSLDYDRADGVSDEGEAVDTLPGNVMF
                     EVSGSGSEFSVNTKINITNDNNRGIIQMRGSGSKVTVSNGGKITINTATTGGFRLQGD
                     SSQIHVSSGGQISVKMAGDDDQPGNNGMRFVGKGLSLIVEGAGSVIDIDKQSGRSSAV
                     RFENGTQKLTVTNGGSLRVRNTGDGKSYVNRLNQGNQAIQFYDASGFLESPGSADFTV
                     TGEKSNIDIRADSGATVDTTGDIDLNFTAGEKTYLVMVGKTGEDKTGIISGDVLNVDL
                     ESPTYFDFQNKRIGSETNGIGGWVFQGNGDSSLKIAHSEIALWRKTGATFDNFEKDPD
                     YFSDMTDLSINGTDLGNFVSSPSAGLTNEFAQTGAGMKNYNRISANNQLPMIESLRVP
                     TNADKKIYGHVVVPEGVEAKPRDAWTGEVEVTLRITYADTTKSSVEITALTQGQTDEN
                     EGAGYNPWGEGEQGGLFEVQVPNDERLKTEDQVQVVAAKKVRGGQEVTELDSAKTTVD
                     VIPPEPANLSTTLINAASRTITGTGEAGALVSLKKNQAVLSETTIDDTGEFVLSIPEN
                     QLFAGDVLEIVLNDQAGEATAKGVQNKPSTNDEIGNHNPSDTPVVYHDAPPFAPATKI
                     VVEGGLSFLAPSKLDFGQIKATGLTQEAFGQMDIQEKLWIYDQRETKNPWILTIKLSQ
                     PFTNDAGFSMQDVLYFKKGEEYQLVNEEELEIVRGQNSTNVQADYSEYLNNGRAFKLV
                     LTKENQIAGNYSAEITWTLADAPTE"
     gene            complement(54244..55128)
                     /locus_tag="SCJ86_000537"
     CDS             complement(54244..55128)
                     /locus_tag="SCJ86_000537"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345340.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diacylglycerol kinase family lipid kinase"
                     /protein_id="HEG1168123.1"
                     /translation="MKKVLLVVNPSSGGEQAKEFEQLAIAKLESVFDEVVVLHTKKAG
                     DAKNFTREAATEGYHSVFVMGGDGTVNEGISGIAEQEHRPNFGFFPLGTVNDLARALG
                     IPLEPEEAINHFSIESVKPLDIGKINDDYFMNVVAIGSIPESINDVDAEKKTKFGKLA
                     YFMSGIKQLASTQSYSFHVEVDGKKEEIESSTLLIGLTNSVGGFETLLPNAKVDDGKL
                     HLVYLKDSSLIDTLKTLPDLLKGVDESTKNLAYLTCEQLVVSLADEEELTTNVDGDEG
                     DKLPVTIKVLPSHLQVYC"
     gene            complement(55223..55660)
                     /locus_tag="SCJ86_000538"
     CDS             complement(55223..55660)
                     /locus_tag="SCJ86_000538"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321308.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEG1168124.1"
                     /translation="MEWFCKFLLFLEVIHVYGFVFLTNKKAGSTMDHLYIKYIHQFSV
                     LVLGTLIIAGIVAFIIQGRDILTVHQEFFSYAFGIPLIAIRSDNKKWKKRYYILLLFL
                     CFYLPPKIFTLLGIQCLSFSFISYIIVLIFLSFIVWMFYLVKK"
     gene            complement(55877..56788)
                     /locus_tag="SCJ86_000539"
     CDS             complement(55877..56788)
                     /locus_tag="SCJ86_000539"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376240.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidyl-prolyl cis-trans isomerase"
                     /protein_id="HEG1168125.1"
                     /translation="MKKRFLALAIVLGTGLLSGCTNAGEKTAVSYKGGTISEQEVMDS
                     LKKMQGADSAVQQLIVYQVFEDKYGDDVSTKEIDSQYDQTKKQLGDSFDSQLKSAGYT
                     EQTFKDSIKQSLAFQEGLKKHIKLTDEDLKTAWESFHPEVEAQIIQVASEDDAKDVKK
                     AADKGDDFSKLAKDKSTDTTTKEDGGKVKFDSTTTTVPAEVKEAAFKLKDGQVSDVIT
                     STNASTYTTEYYVVKMVKNQNKGNDMDKYKKELKEIATDTKLSDSTFQNKVIGEVLKD
                     ANVKIKDKDFENVLSTFTSDSSTASSK"
     gene            complement(57245..57673)
                     /locus_tag="SCJ86_000540"
     CDS             complement(57245..57673)
                     /locus_tag="SCJ86_000540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288545.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEG1168126.1"
                     /translation="MKKAVIICALGIGFVGGNAMSSEVEANSVHGEIYYKSLKPGEIH
                     FDAKGNIIKSNLPVDREISRTAINHSSGRWVYYSDTTCTRKTGHSNHYSRVYNRHGAK
                     AKVGNSIRSANATKGVWAYASATGNRTDTFSCWYNPTGWY"
     gene            complement(57701..58339)
                     /locus_tag="SCJ86_000541"
     CDS             complement(57701..58339)
                     /locus_tag="SCJ86_000541"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEG1168127.1"
                     /translation="MEPLIVIKGAKKIIRNKTILHLDRFTADAGDLVTVVGESGAGKS
                     TLLNILGLNDQFSSGQFYLFDQETKQLTGREKRLIKRNTISFLFQDFGLIEYETIDFN
                     LDIGLKFSNLNVSDKRTAKYEALKKVNINKKLSTPIYTLSGGEKQRIACARVLLKNSK
                     IILADEPTGSLDKRNKDKVVEILSQQASQGTLVIVVTHDPYLTKIGNKKIML"
     gene            complement(58343..60325)
                     /locus_tag="SCJ86_000542"
     CDS             complement(58343..60325)
                     /locus_tag="SCJ86_000542"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015370994.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1430 domain-containing protein"
                     /protein_id="HEG1168128.1"
                     /translation="MSGFALVSINKQVLYRTNEFFHQTDTYTPIRLPQHFIGQEEYEQ
                     LIMVFENVSSETELSYMKKTVNESNEVDTSWNFKIQPKLEMTLYYQGDVKSSHEKVPY
                     SNTSFRVTSIEESIKIKDFEGEFFLMTREKEAYERFISLFTDRFNQAFHTDYATNIFE
                     DFENSYSYSVISNTDVYNYRVYLTIGLILLFLLLSSFFLLMNKEISLLLNNDFSLHQV
                     LWCLLGKKKIIITFIIAGIIGGFIFMYYKEQFLYLFQMYVFLFSGIYGISLISLLLVE
                     KIASLREAFNYIQYILFTFGLLFSLLLTVANVELATIVFSSSQLAVSHEPPKEIAGED
                     YHVFYPVTIGKNHVDFIYSNRFASAADQELYETLNKNGSILVNVSDYLNEENEQFGRG
                     IKINLNYLKKFPLIDVSGRLIQITEKETHPVILIPERYRHTDLKNDLAQITEYYQEIS
                     EKEPKFLFIKNNQPIYTFIPSIPWIEHYPVVEILTLKNSMYLERNILSGEIYPPLKIK
                     IGSLSKERLFELIEESQLRDNLQLSFPYTQTEEIAVKVLSRSFHYLIQIFLLTFFSFF
                     LLSYVMLCIYFVYNCRKIAIFRSSGYSLFETYKDFFMMNLIKWGTTSVIFLFLIEREP
                     KYLFNIFFFSFIDFILSVVFILSTEKKSQLLLMNGG"
     gene            60505..61170
                     /locus_tag="SCJ86_000543"
     CDS             60505..61170
                     /locus_tag="SCJ86_000543"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347318.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HEG1168129.1"
                     /translation="MNTGEKIRTYRRNCKLTQKELGEKIGVSDKTISSWENSRTMPDL
                     EMLSLLHHTLGLPIDFPSIESATESIEPTLPDEPLVNKKSYSSKQPFSLKKTLSFFLL
                     VFCLVYLFLINYQYKNETIDRFNPFLKPQIGYTTLPVGYVDNSIERWVCNDIFGHGTW
                     LSFHAGEVEKEKNHAVIKHKGKHVMNVRLLTWEAIPSDYQEILSQDYDDALKRSPQEP
                     DRH"
BASE COUNT        18348 a        13052 c        10680 g        20867 t
ORIGIN      
        1 tttttttaaa tgattaataa ataacaactt ctttctatga aattgcaaag cattcatctt
       61 cttttccgct aacgatatat ccgtcagtgc ttggtacact ttactaaaaa gtatgagatt
      121 gccgatatct ggataggtgt gctatactac agaaaaagat atacaatctg ccaccgggta
      181 ggataaagag gatgatcatt ccattaggtc tttgaaggga tgattttcct ctttttgtgt
      241 atttttttat agttaaggag gaatgtaagt atggcatgga tggaattaat catagcagga
      301 attttggaag tattctggtc aacgatgatg aaatggagtg atggtttttc taaaatcaat
      361 tattcttttt acacagtgat tggcatgatt gctagttttt actttttgtc aaaggcaatc
      421 aaaagtttgc cgatgagtct ctcttatcct atctggacag ggattggcgc agttggttcg
      481 gtcataattg gtgtggtctt gtttcatgat aagctgaatc tttccacttg gttctttgtg
      541 gggatgctac tcatcagtat cattggcatc aagattacta gcggccacta gacacttgga
      601 ataagtactg atcagaaaaa ataagccttt ttatttaata ttcaattttt ttatgaaaaa
      661 ataagaaata agtccaatga cctagcgact tctccactca tcaggtgtat gcttgtacta
      721 tctatagaaa aggagtgaat atttttgaaa agtctttcgg tccaagaatt atccaagaac
      781 tttggtcaca aacaagccct cagcgatgtc agttttagct tatcccctgg caaaatcgtt
      841 ggattagtag gtcctaatgg cgctggaaaa acaacgatca tgaaagctat tttaggactg
      901 atccattact cagatggaac aatctgtatt ggtgaaaatg agatttctcc ctctagtcat
      961 aaagggttgg aacaagtagg agcattgatc gaatacccgg gtatctatcc attcttaaca
     1021 ggctatgatc atctgaagtt attctctgaa accaacgatg tatctgctat cgacactatc
     1081 gtgaatcagc tcaagatgga aaagtacatt cacaaaaaag cgaaatccta ctcgttggga
     1141 atgaaacaaa aattgggaat tgctcttgcc ttactgaacc atccgtctat cgttatttta
     1201 gatgagccga tgaacggttt ggatccacaa gcaacaagag atgtgcgaaa aatcattacc
     1261 gacttggcta ctcagggcac ctctttcttg atctccagtc acttactcag cgagcttgaa
     1321 aaaatggtcg atgagctgat cctgatcaat caaggaaaga tcatcaaaca atgtacaatg
     1381 gctgaattga accaaacagc gcaagatttc atcgttatct ctacaacaaa tgatctagct
     1441 gctcgaacga tcttgacaga agctggatat actttggaag atcctgataa aatcaagctt
     1501 ttaaaagaaa acgaagaatt attaggaacc gtgatccagc tattgtctgc acaaaatatc
     1561 catgtgacag acgtccagca tctgacgaat gatttggaat cttctctatt ggaaattctg
     1621 gattcagtaa agggggaaga agaaaaatga cattgcttgt aaaacaagaa ttatttaaat
     1681 tgatcaagaa aaagtcaact gctgttttat ctgttctatt agttgttttg ctcataggaa
     1741 cagcacttct agccaaaaaa tacacgacga tcattgatcc agttgaaatg actgctcagc
     1801 tttttagtgc cacatcttgg attgtcttta tcatgattgc agctgcaagc acgatcattt
     1861 cgatggaagc acaatatggt acattgaaaa atctccttta tcgaaagtat tctcgaggag
     1921 aaatattagt aagcaaatgg atcacattag ttatttattc tgtttattta taccttttag
     1981 ctattattgt cactgtgttg atgaaactga ttcttttccc ttcgatttcc ttcacggaga
     2041 aggtttctac tggtcaaaca ctgatccaaa gcttgttcac ttatactttg ggtagttata
     2101 tcggtttatg gctgatttta agtttagtgt tgatgattgc ttgctttatc aatagttccg
     2161 gcgcatccat ctcagcagga atcgtctttt attttgcttc gtcaatcata tctggtatcc
     2221 ttattgcact catccaaaaa tgggagtgga tcaaatggaa tccgatcagt atgttgaatc
     2281 tgcaaaacca aattggaaac gaagagatca tgaagcaatt gactcatctt tctactaatc
     2341 aaatgctatt cggtaattta gcttacatcg ttttgttctt agcattagga tatctcgtat
     2401 ttaagcgaaa aaatgtatga tgactcatca aaaaagaggc tgtgaaagag acgttcagcc
     2461 ttattgccga ttggctgtct cttaaacacc gtttattctg aaaaaggagc tgtgacaaca
     2521 tttgtgtcac agctcctttc ctagtatgtt tttgaataaa cgacaggctc acaacctctc
     2581 tttatacttc ttttatactt ctatacgtga agcatcccag tggcaaataa aaccattgca
     2641 atcactccga ccatcgtcac acctataagt aaaatcttat ctaagtggat atgttgtaca
     2701 ggctcgtttt gctccttacg tgctttgagg taaaaataaa tgcctggcaa gaaaagaaca
     2761 agaacaagca ttacttcatt aaaggctacg actagcatag cccatacttg aaaggcactt
     2821 gctaaaagcc ctactaaaaa ttggacccat tctttccgct gataagaaag tttcatttgg
     2881 tacaatccta agaaaagcca cgtgaagaaa atagctgctg taccaagtgt gtaagcaaag
     2941 ttataagcac tgtcagtgaa taataatgaa agtaaaaaga cattcgtaca taacgtcgtc
     3001 aatacgatag cataagttgg tgccccattt ttgttgagtt ttccccatgc tttaggcaaa
     3061 agtccgtctt cagccatgtt tcccattgtt tcaatcggta agatcgtcca tgagagccaa
     3121 acaccaacta aagaaatgat caaaccgccg ttgatcacga ctgctcccca tttaccaacc
     3181 atcatttcaa acacatagcc catagcaggc tgcgccttga tcgtttgcag ctgattcatc
     3241 gtcattgtcc catatggaag catggaaacc aagacgtaaa taataatcaa gccgaatatc
     3301 cctagaatcg ttgctttccc aacatctgat tgtttttttg ctcgtgaacc tacgacagag
     3361 gctccttcaa taccaacaaa tacccaaagc atcaccatga tgcaactcaa tacttgactg
     3421 gaaaacggga tcggctgtcc attcgcattc aatataatgt cgctagaaaa gttatcaatg
     3481 aattgttgga tcgaaaacgt gctgatagct actacaataa ataaaaataa tggcactaat
     3541 ttacagaatg tgacgatagc attgacgaaa ctagcatttt cgactccccg attcactagc
     3601 catgcatatc cccataaaaa gactgtactc atcacgatcg atgccagatt ttgcccacct
     3661 tcaaatactg ggaagaaata tcctaatgcg ctcatcaaca atgttgcaaa agctacgttg
     3721 cctaaccagc atgtgagcca gtaaccccaa ccgctgataa aaccagcgaa acggccaaaa
     3781 ccttcttctg cgtaactaaa gatcccgcct tttaaatctg gacgtttttt attgatatta
     3841 ttgatcgaca aggagagcat caacattgct gttcctacga gtacccaact gaaaatagct
     3901 gggccgccag ccgatccgga gatgtccgtc ataataccaa atacgccacc tccaatcgct
     3961 gaaccaatta ctaaggcagc aagtggaatt aaaccaattc cttttttctg ttgctctgta
     4021 tccatgtaag catcctccaa aataaaaccg cttttatcaa aaagtataac aaaccttttt
     4081 tatttttact taattaataa aaactttaat agaaatataa ttctaccggc ttttttctca
     4141 ttatacaagc aaagcgccta gtccctttcc aaattacacc accaaaaaac ttaaaaaaga
     4201 tgctatacta aaaggaaaga attggaggat gatccatgaa cattttatat atatcgattt
     4261 caggaaatac gcgtgccttt gccaaacatc tagcagaata tgctgaaaaa atgcatgcag
     4321 aagatcctgt caatcctgaa gttactttaa aagaaatcca tgaaaatagt gattttacga
     4381 aagaaacaga accatttttt actttcgttc ctacttattt agacggcgga aatggtttag
     4441 ataacggtga tacagaaatc ttgaccgaaa cgatgcgtga atatctggaa catgaagaca
     4501 atcataaact ttgtcttggt gtagtcggta gcggaaataa aaacttcaat aatcagtatt
     4561 gtctaacagc caaacaatat gcacaaacat ttggctttcc tttcttggca gattatgaat
     4621 tacggggaac accatctgat gtgacacgaa tttatcagat tttagcagaa aacaataaat
     4681 aataaaaaag aagaagctga aaaatagatt tcagtttctt ctttcgtttc tattgtttct
     4741 atcatattaa gctttgacat ccatcacagg tgcttttgct tttgctttac ctgttttttc
     4801 caagacaaag ttttttacac gatccatatt ggttaccaag acgatcatgg atgtttcttt
     4861 gcctgctgct ttgatcgctt ctaagtcagc ttctgctaca agcgtatcag gtgtcacttg
     4921 ctgcccttct gttactttca catcaaatgg cgtaccgcct aagtcaactg tgttgattcc
     4981 catatgaagc aagatttcta aaccatttgg cgttgtgatc ccaatagcgt gtttcgttgg
     5041 gaagacagag ctgactaccc cagtaactgg agaatagata tgtccgtttt ctggttctac
     5101 aaagtagcct tcacccatca tcttttgtgc aaaaactgga tcacttgctt tatctaaaga
     5161 aacaactgtt ccgtcagcaa ctgatgagag gatagtcaca ctttcagctg aagtttgagg
     5221 tactactttt tcaggcactt catttactgt ggctgtttca gtcgctaatt cttctttttc
     5281 taaacggtct ttaggaatac cgaagaagaa tgtagcaacg aagccaccag cataagctgc
     5341 caataatcca aggatgtagc ctaaccattt accgtcagag atcaatggga tcaaagcggc
     5401 tcctgaaggt ccaatagcga tagccccgac atttccgatc attccgatca ctgccccgcc
     5461 gatacctcca ccgatacaag cagtaataaa tgggcgtcct agaggtaatg ttactccgta
     5521 aatcaatggt tcgccgattc ctaggatccc gactggtaaa gctcctttga tcatttctgt
     5581 taattcttta tcttttttgc agcgaatcca taatgccaat gctgcaccca cttgtcctgc
     5641 accagccatc gcaagaattg gaagcaatag cgtacgacca gtttcatcaa tcatttgcac
     5701 atggattgga gtcaaaattt gatgcagtcc gaacataacc atcggcaaga ataatgtgcc
     5761 tagaacgaag ccggcaaatg ctccaccaac gccaagtacc cagttgatcc caccgaccat
     5821 gccatcagaa atgaaacctg ctaaaggcat gatgaagaaa atttcaatca gaccgatggc
     5881 aatcaatgaa agagttggtg taacgatgat atctaccgca tcaggtacta ctttatgcag
     5941 ttttttctca acgattgaca atagccaaac agcgaagatg acacctagga tcccaccttg
     6001 tccagctgca agagcttcac cagtaaataa gtttttcagt ggtgcttccg gattcatccc
     6061 tgtcagcatc acaacggccc cgataactcc accaagagtt ggtgttgcac caaaaacttg
     6121 tgcagcattg atacctgtat agataactag gtagctaaac atcccgtttt tcaagatgtt
     6181 catcacatcg atatattgtt gccatgttgc tgcatcaagt gttccggctg tgactaagtt
     6241 tgctaaaact gcggcaatcc ctgccacgat cccagtaccg acaaatgcag gaatcattgg
     6301 cacaaagata ttagaaatgt cttttaatac cgatttgatt ttagatggtt tttgtttcga
     6361 tttttgtgcc gctttcattt cttgtgcccg ttcgtttaca cggtcttttc cagatgctga
     6421 tgaatttgct gtagctgtac ttacagaaga aagtttttct cctaatttca cgcctgccaa
     6481 atcaaccatt tgctgtgcta ctttgttcac tttcccaggt ccgatgatga cttgaagctg
     6541 ttcatcttct actacaccaa gcactcctgg aatcgctttt aattcagaga catttacaag
     6601 atcatcattg atgatcttca tacgcacacg agtcatgcag tggacaatgc tttctacatt
     6661 gcccatgccg cctgcccctt tataaatttg ttttgccagt tcttccaaag tcagttcgtt
     6721 ttttgccatg gtcttttccc cctcataagg tcccttttac aaatccgttt gcttcagcta
     6781 attttgcttg agcttcctct ttatcactat ttgtcaaaat catgacgata gccagcttaa
     6841 cattttgatc tgcttcttca aagtactttt cagctgtttc gtaatcagct tctgtcgctt
     6901 gcatgatgat tcgttttgag cgttcgatta gtttttcatt tgttggtttg acatcgacca
     6961 tcaagttatt gaatacttta cctataccaa tcatactgat agtagaaatc atattcaaga
     7021 taagtttttg tgctgttcct gacttcaatc gagtagagcc tgtcaaaaat tctggacctg
     7081 catcgacttc gattgggaaa tccgcgtgtt tactgatttc ggcttgttta ttgcaagaaa
     7141 tcgttcctgt gacagcaccg atcgattttg cgtaatctaa tccaccgatc acatatggtg
     7201 tacgaccgct agctgcgata ccaataacta tatcattttt tgtcaaattc agcttgatta
     7261 gatcttcttg ccctagctca taagaatctt cggccccttc gacagctaca gtcattgctt
     7321 cttctccacc agcaattaga cctatgacca tttccggttc tacaccgaat gtcggtacgc
     7381 attcagcagc atctaacaca cctagtcttc cacttgttcc ggctcccata taaatcagac
     7441 gtccaccttt tttaaaggac tcaatcgtgt tagcaatcac tggttcgata cttgtcagtt
     7501 ctttttcaac cgcagcggcc actttctgat cttctttgtt cattagttga acggcttgag
     7561 aaatactcat ctcatctaat ccaaaagttt cttggtttct gcgttccgtt gttaggttct
     7621 ctaaattgat tttgctcatt tttatcttcc ttcttttctt ataaattgat agttcatacc
     7681 agctttgatt tggttgatca atggtagctc ttctttgata actttagcta ctacattcac
     7741 tttttcatcc gctggcaaat catatttggt taattggatc tcacccatat agcgcaaata
     7801 cttttcgtta tctaaagtaa tagacccttt catacgcgta gctgtatttc ttgctgggat
     7861 aggcggaatc tctcgaaaac gtgcatctac gcttcgtatg acatctcttg catcgtcttg
     7921 ccgattcgta tgcttcccta atatatattc gtaaaattct tcatctacag gttccgtatg
     7981 gagcaagact gtctgttctt tttggaaaga agcaaattgc tcctgaacgt tttctgacag
     8041 acctgcatct ccgatgtaaa caatatccgt gttacttgct aatagatcta gagcgcctgc
     8101 aagaggatgg atccctcgat gttcttccaa agttggcaat ccttgatata gtgggcctct
     8161 caactctgta tctcctggta caaaaccttg gacaaggaga ccttgaattt tgagccattg
     8221 attttttttc tgataccatt ctttgtccag ccctgtttcg ggacgtggat aataattatg
     8281 ccaagcttca atctgtgaga aatcagcttc tgcttctttc aattcatcga tatctttagg
     8341 cgtgatcgtg ctagcattca aactgatttt caattgatgt gaccagtcgg caatctgctg
     8401 attggaaata tgataatcca ttcttaaacc tgttacacca attgctttca aaggcgttag
     8461 atcttctaat gaaaaacccg ctcgttccaa tgcctctcct gaaatatcga ccatcaattc
     8521 tagtttattc tttttcgctt cgcttcctag ttcgatcaat cgcttttgat atgccgaaac
     8581 atcatcttct ggtatatgca tggaagtgaa aataccgaca aagccattgg atgccatttg
     8641 gcggatatac tgtttcttct cttctgacaa agaatcgttc ataaatattg aaaagccaaa
     8701 catattatcc ctctttttct gtatccgtct tcatttctat gactagagta tatatctaaa
     8761 gaaacaaaat ttcaatactt ttattaaaat aattaaattt catttctaaa ggttgagttt
     8821 atattatcaa aagaattgtc ataacctaaa aaaggcactg ctaagcaaaa gcagtgcctt
     8881 gaaatatttt atttaaataa cacaaaaaca taattactca taagaaacca attctgtatt
     8941 atctaccatg aatgtaaaga acccctcatt aggcatcgga tgaaaataag cattgatcac
     9001 tcttatcaag gcattatggc aaactaacag atagacattt tcttcgtctt cgatgcattc
     9061 tttcaataaa ggaacgatcc gtgcatacac atctaagact gactccccat ttgggaatcg
     9121 tacagcgaac gctaaacgtg ctttttgaaa gttctcatct ttgcttggca gtccatcata
     9181 atccccaaaa tccatttcta ccaatcgttc atcaacggtc agcggcaagc tgagacgatc
     9241 agcaactgct tgtgctgtat ctcttgctcg ttgaagaggg gaatggatga ttttcgtgat
     9301 tggaacaggc aaatctacta ccttttctgc tagtttctgt gcctgcatct gtcctttttc
     9361 agtcagcggc acatctgccc gtccgcaaac taatccttgt gcgttccaag ttgtttctcc
     9421 gtgtctcgtt acataaagca tgtgattctc tccttcgttg gcaaagttcc ccttaaccat
     9481 accttgttgt tcttgagatt acaagggtta gattctctca agagaaataa tctcagtaga
     9541 tactttatcc aggaaggttt gatcatgttc cactagtaac atggcaggtt tcacgttcaa
     9601 gatcagttgt tctaattgtt cttgattgaa gacatccaaa taattcaatg gttcatccca
     9661 tgtatatagt tcagctggct gtgataaaga tttagccaat tccacttttt tccgttggcc
     9721 catactcatc tgctcgatct tgttatgaaa aacatctctt tccatcccaa gctttcggag
     9781 gttgttcaaa aatgcttggt agtctacttg gtttttctct gcaaattccg ccaacgttcc
     9841 tcgattgtct tcataatttt ggcttgcata actaatgctc agatgttttg gctggtattt
     9901 ttctcctata accttgccgt tgaatgcccc cagaagataa tggataatgg atgactttcc
     9961 tgcaccgtta ggacctgaaa tcgccacccg ctgatgaggc tcgattgtaa aatgaattgg
    10021 ctcgaataac agattttcat accctaattg aagatcttct acgcttaaaa gtcgcttatg
    10081 gtgagacgct tggctattca tcgtcaaaga atcgatatat tcgatatctt ttaatagttt
    10141 ttctttctcg ctgatctggg tctccatccg attcacgatt gctttggatc gtttcatcgt
    10201 ccgtgcagca tcagcaccca ctgctccttt attcactcgt ctagattcag tatcgatgaa
    10261 tccgacttgt ttctttgttt tatctccttc tcgggaacga gaccattcgg ctttttcagc
    10321 tgctgttttc tttagcctac tgacttcttt tttcaatttt tcattttgag ccatttcaaa
    10381 ttcatcacga agttttttct gttcttcata gatagagaaa ttcccttgat aaagttccag
    10441 ttgacttttt tcgattgcta aaacatggtc cactacttca tcgataaatc cccggtcatg
    10501 gctgaccacg atgaagcctt gttttttctt tttcaaataa gccgctactt gttttctacc
    10561 agagatatcc aaatgattcg ttggttcatc gattaacggg aaatgagtgt catccacaaa
    10621 taaaagtgcc agtaggactt ttgtcttctc tccccccgat agtgtgctga attctctcca
    10681 taagatttca ggatctgttt gcatcaattg gagctctctt tcgatttccc atatctcaaa
    10741 atccgtaata tcatttaaca cgtaataggt taaacgttct ttatcttttg tctgttgcgg
    10801 gaaataggca aattcttgct gatggattac ttgcccttga taaggtagtt tgttttgtag
    10861 aatattcagt aaagttgtct ttcctcgacc gtttcgtccg ataagtccta gtttccattg
    10921 tgtgtcaaaa tttagatttg cttgttcaaa taataatgtg ccttggctgt cgtagccgaa
    10981 tgtcagattt tttatttcga ttttagacat tttccatcac ctcgtaaaaa aagaccagtc
    11041 ctattttcag aactggctaa agaggatgca agggttggat acacacaaaa agacccatac
    11101 atcgaaaaaa ccaagccaat cccgaaaatc tgcacactgc ttccttgtct ccacaaggta
    11161 cagtctgaat gcagctttca tcaatcgact taatttttca atgcacggat ccctcacttt
    11221 gtcctatatt tgaaattacg atagcataaa gaaaatgatt ttgtcaacta cttcgggagg
    11281 atggtgaaaa atctgctaag tgtctgtcct aagatttttg ttttttctaa tgtttgcctg
    11341 tgttccattg cttcttctaa acctattgga cccaaatgaa tacttaagac aattgggaga
    11401 aaatcagaaa gtccttctag ctcttggtct ctacttccgc aaatagcgat tattggtttt
    11461 cgatattttt ttgcaagcac tgctacaccg tatggaactt ttccgtggat cgtttgctga
    11521 tcgatccgcc cttctcctgt aatgatgaca tctgcttgtt cgatcttttt ttccaatcca
    11581 gtgatttggc tgactaaatc gaaaccagaa accatcttcg ctcccaacaa aaaaagggct
    11641 ccaccaatac cgcctgctgc tccacttcct ggaatatcat ctaaatccac ctcccagaaa
    11701 tcttttattt gctgagcaat ctttcctgct tgtttatcta agaaagctag cgtcgtatct
    11761 attcctcctt tttgtcttcc aaattgccaa gcagctcctt gttgacctgt atatggattg
    11821 gtaacatctg cagcgataat caattttact ttagaaacaa gaacgtttct tcgatcaaaa
    11881 tgaagttttt tagtagataa taaaggattt ccttctgtgt acccttctaa agaagcaccc
    11941 aaggattgga gtaatcctag accaccatcg ctacacccgc ttcctcctaa agtgacaata
    12001 atttgttcta cttcttgaag cagtgcatct ttaataagtt ctcctaaggc ataactggaa
    12061 gcaagtcgga tagtttgatc cgttggttta tcaagtagat ctaaccccaa tgcttgtgca
    12121 gattcgatga ctgcggtgtt ttttccttgc caagttgtca ataaatagct gatttttcgc
    12181 tgacggaata agagatccat cccatcaatg gtacgccatg ttcccctgag cgcgtaatag
    12241 attgcttctg cagtattttc tccgccatct gcaatcggca cattgatttt tcttccttta
    12301 cgatcttccc agccatttag aactgcctga tttagttcaa atgaagaagc acttccttta
    12361 aatgaatcaa ttgccgccaa aatatccatc tgtatccctc actttcttct taagtaagac
    12421 actttttttc atttttttca aacaaaacaa tgctattttc ttccattttt ctatatacta
    12481 aaagggaact cattgaataa aggagagaaa aaacatgaaa atcgcaatag ctggggccgg
    12541 agcaatgggc agccgaatcg gattgatgct acatcaaagc ggaaatgagg tcctcttgat
    12601 cgatcgttgg cctgctcaca ttgaagccat tcgtacaaat gggttgattg ctgactttaa
    12661 cggtaaagaa gtggtcgcaa aactgccaat ctattcacca gaagaaatca tcgaaagcaa
    12721 cgaacacgtg gacttgatcg tagcattaac aaaagccaat caattagatg atatgttttg
    12781 ttcgatccaa tccattatta ctgacaatac ttatgttttg tgtttactaa atggattagg
    12841 tcatgaagat gtactggaaa aatatgtgcc aaagaaaaat atcttatttg ggatcacgat
    12901 gtggacagct ggtctagctg gtcccggaaa agtcacattg ttaggtgatg gagaaatcga
    12961 actagagaac ttggaaccag aaggcgaagc atttacaaaa aaagttgtag aagtctttca
    13021 agaagcaaat ttaaatccga tctatagcca taacgttcgt tattccattt ggcgaaaagc
    13081 ttgtgtaaat ggaacattga atggtttatg cacgattttg gattgtaata ttgctgaact
    13141 aggcgcgcaa aaagcggcag agagcatggt tcgaacaatc gtatctgaat ttgcttctat
    13201 cgcagcaaaa gaaggaatca tactagatca agaagaagtg taccaacata ttgcttctac
    13261 ttatgatcct gacaacatcg ggttgcatta cccatctatg taccaagacc tgatcaaaaa
    13321 ccatcgtttg actgaaatcg actacatcaa tggtgccatt tggcgaaaag gacaaaaata
    13381 tgatattgct acaccatatt gtgccttttt gacccaatta gtccatgcaa aagaaggaat
    13441 ccttggcgca gaataaaagg ctctctgatg agaatcagct gaatcagcag ctggttctct
    13501 ttttttaagc ccaataaaag atcaaaaaga aaacacaact ctcatgatgc cataagaatt
    13561 gtgcttgttt gtttcagtac ataatctctt caaaatgtag gttttttatt tgttttcaac
    13621 agaggagagg tcatattcgt attgctcata aatagtggaa tcgttagtga taaatcgttt
    13681 tgcccaattt tctgcatcat aaaattccaa tagctctgat aggattggct taaatggtgt
    13741 atgcagaaat tccggtaatt catctaaatc acgttctcga agcaaaaaaa tattttcagg
    13801 acgataaaaa tgataatgag caatcgcttg attatccgtc actagtttct tttgaaaata
    13861 catggattca aatgtccgca aagtcactcc ttgctgaccc tcccgcaaca gttccagaat
    13921 cccgcgcgat tcccctgttt ttttcaggta ctcttcataa gtaagatatc caggttgttc
    13981 attccctcga tgtggaagaa tgaaatagtt ggtcgtaaca tctcttttta taaattcctg
    14041 cttatagctt tctgctcgtt cttttctccc attattcgtt gcaccaaaaa atagatccgt
    14101 ctttggttcg ctagaagaaa aattcatatg tttcgaataa aatgaactat tatgtttcag
    14161 cccaagttct tttgcttcca aaaaatcaaa atgataaatc tcatcgattg ccggttcatc
    14221 ttgtgcctgc ttcagcatcg cctcgtgttc tgctttgaag tgattccaaa aaaataacac
    14281 aatttttgca ttggtaaatg ggcgcacgcg tcgagccatc tccatatcaa atccataatc
    14341 taaaaaaacg atcagctgat aatttgccaa ctgttgtcta gctgatttaa tgaattccga
    14401 ctgtagtaaa tcttttccgg accaaacatc taaagagcgg atggaaggca aaaaataaag
    14461 tgatttattt tttgcaacta gcagtacgtc atttttgttc atcttttccg cctcactttc
    14521 tctgtcactt tttcctatga tagcctgttt aaatagtaaa aagccagtaa aacgtttcct
    14581 atatttgtat gaaaatttaa catttgtatg aaaatatgat gaaataaatc attcttctat
    14641 taaaattcgc ctaaaagttt tctgaatagt tgtaattgtc tgaaaatttg ttatcattag
    14701 tcattgttaa atattgctat ttattcttaa tataaatttt gattgtgaat ttaaaagaaa
    14761 gaggtcattt atggaagata aaaaattgaa aaaacaactt agctccagac atatcaccat
    14821 gttagcttta ggtggagcaa tcggtgcagg tctatttaaa ggaagtggcg aagcgattgg
    14881 gatcgccggc ccttctgtat tgattgcttt tttgattggt gggctgattt tatatatcgt
    14941 gatgaaaggt ctgggaaaaa tcgttttaag cggtggcgac acgcatcacg gattatctgg
    15001 tttgatacgc ccttatctag gtgctcattc tgctgatttt actgattggg tctactggtc
    15061 tatgtggatt atcaatatca tcgctgaagc tgttgcagct gcttcctttt tgcagttgtg
    15121 gttcccgcac attccagcct gggtatttgt ttttatgttg gctgttttaa ctacgatcat
    15181 caatctttat tcggtacggt tattcgcaga aacagaatat tggctggctt ttgctaaaat
    15241 cagtgtcatt atcttactga tcatttttgc tacttatctt gtcggtcagc aaatgttggg
    15301 cacaggcgtt ttccctaccc ttcagcaatt gactgaccat ggtgggttta ctcctcatgg
    15361 tatgaaggga atcatcagtt ctttgcttgt agttatctat tcttacggcg gttctgaact
    15421 gattgctatc acggtaagcg aagcagatga tcctaaacgt gcgattccta aagcaatcaa
    15481 aggtgtcatg ggaagaatca tttcctttta catcattcct ttgtttttgc ttttgattct
    15541 ttttccttgg aatactttag caggcacgaa tgtcagtcct tttgtgatgg tatttgaaaa
    15601 aatgaatatc ccatttgcag cggatatcgt caattttgtg attgtccttg ctttgttctc
    15661 atctatcaat tcgggtgtgt atgcatcttc aagaattttg ttcttccgtt tgaaggatcg
    15721 aaaaggttcc tctagaaaac tagctgtttt aaacaagcat caagtaccgc aacgtgctgt
    15781 tttattttgt accagtgttc tttatcttgg tgtagctctt tcttattttg tcggtgacaa
    15841 attatttggc tatctggcag gttccctttc gtatacagta ttattgatct ggatcattat
    15901 cagtgcggca tcctttgtac tggcactaag aaaaggaaca ttatgggata aaggaatcag
    15961 cttttttgca ttagctgtct taggattgat tttccttggt atcctgttca cgaactccat
    16021 tggggtaact gtattgactg gcctgcttta cctgttcatt tatttcagtt atcaaaaaaa
    16081 gaatgatgcg ttcgtattga cgaatgaatg aaatcgatag aaaattttat tttatttatg
    16141 tggaatagat ggagctattg aagaaaggag ttgtgacaaa agtcttgttg caactccttt
    16201 ttccgaataa acagtctcat gacctcttgg taaacggtgt tcaaaaaccc tctgtgcaaa
    16261 ataattgatt gttctccatg ctcatgacat gatagaagaa aggaatgaaa aaaaggagtt
    16321 tgttttaaat gaaaaagtat tggaagtttt tgttgatgat cggtgtttca acggtagtta
    16381 tgtttggtat gatgtacttg aatgtctatg cgctcgatca tatcttcttc agtcgtacac
    16441 gagtattcat ggcgttgatg atgggtgcag tcatggcagt catcatgctg ttgttcatgt
    16501 ggaaaatgta tgaaaacaaa aagataaatg ttatgatctt agccgctagt cttcttgttt
    16561 ttgccggttc cttgtttatg gtacgaagcc aaactactgt caacgacaca gcttggatga
    16621 aggccatgat tcctcatcat tctatcgcta tcctggcaag taaacgagct gatttaaaag
    16681 atcctgaagt aaaaaaatta gcggaagaaa tcattcaggc acaagaagaa gagatcgaca
    16741 aaatgaaaaa aatgatcaaa gaaatggaat gaccattatc cgtccaaata aaaaaagagg
    16801 atgttccaga agttatcagc ttcaagaaat aagaagaagc tacttctgga acgccgtgaa
    16861 taaggggttg tgaaaaagct gccctgcatc aagtaataag aacagccaaa caaaaatagc
    16921 ttctcctatt tttcgtttgg ttaggcttat taccgcagat gcaagctttt gaacaccgtt
    16981 tattaagagg ttgtaaaatc agcgttctga cctgagtatt agagagacaa atggaaaaat
    17041 agttcctcat attttgccat tttttgagct aataccgagg gtcagctgat tgaacaccgt
    17101 ttatcgagag gttgtgaaaa agtcgcttag tttcgcgtat taagaaaaat tttgtgaaat
    17161 ggctttccac atttttgtat aaatttggct taataccgag aaactggctt ttgaataccg
    17221 tttattcgta aaagggattg tgacaaaagt cttgtcacaa tcccttttta tgtttttatt
    17281 gttctaattg cgctgtcttg cttactgctt cttgcggttt cacaaatttt tttgagattc
    17341 cccaacctgt gaaggcactg accatcgaaa acacaggaga aagcaaactg aaaaatacga
    17401 atggcaagta agtgagtgta ggaataccaa atgtacttgc tacgaaactt ccggcaattc
    17461 cccaaggcac taagtagttg attactgttc caccatcttc tagcgttcta cctaatacag
    17521 ttggatcaaa tcctgctttt tcataagttt ctttaaatgc attaccaggc agaataacag
    17581 ataggaattg ttcaccaata aaaatattta ctccgatact tgtcatcaat gttgtaaaaa
    17641 tcaaatttgg tgtgttcgtc atctttcttg atactcgttc catcacggtt gagatcaagc
    17701 caaattcgat caacaatcca cctaatgaca aagttagaat aatcaatgac acagtaggca
    17761 tcatactttc gattcctccg cggctaagca gtgaatcgat ttgttggttt ccagtatttg
    17821 aaacaaaccc tgattcgatg accccagcca acttcgttaa ggaagtttgt gggttttgaa
    17881 taaaaatcat tcctactgct acagcgatat tcaacaaaat cgttaaaatt gccggcacct
    17941 ttttccatgc acacagtaac attaaaaata aaggaatcag tgaccagatt gaaatagtaa
    18001 agtttgcttc tagaacatca gtcacttcac gaatagatga aagacttgcg gctttacttg
    18061 tgtgtcctaa aatcatgaat aaaaagaaag aaacaacaaa tgccgggaca gttgaccaca
    18121 tcatatgctt gatgtgtttg aacaaatcgg catcaacaat agctgctgct agatttgttg
    18181 attcggataa tggagacatc ttatctccaa aaacggcacc tgaaacaata gctccaacaa
    18241 ccagcgctgg atttaagcct agtgttgtcc caataccgaa aaatgcaatc ccaatcgttg
    18301 acataacagt aaaggcactc cctacagcgc tacctacgat tgcacacaca acaaatacag
    18361 aagggacaaa ccactggaca ctgatcaatt tgaacccaat gaccattaga gttggaatga
    18421 tccctgcttg tatccataca gcaatcaatg aaccgactaa aataaaaata aagattggga
    18481 tgattcctgg tttgatgcca ttgatgattc cttcgtgcag ccaatctgtc gggtatcttt
    18541 ttatcactgc atacaacatg accattccga tcgctgctaa gatcgtgata ttaggtgaga
    18601 cctttaatcc gacaacgcca atcgcaatgc ctaaaacaat aaccattaat acaagtgttg
    18661 cttctaaaaa acttggtgtt cctttttttc ccatgataat ctctcctttt ttagttgttc
    18721 ttatgttcta aaaaagttaa gatatcggat tactccgccg caattcatcc gcgattcctc
    18781 ctttataaaa ataaaaagac ctgttttaaa ctaaggacga atgactccgc ggtaccacct
    18841 taattaaaaa cagatctgtt tttcactttc aaacttttat tcaaatgtat ggactttcca
    18901 tgtaattcgt caattaggct tgatcggttc acagcgacca ccgactttct gaacaaaaca
    18961 cctaaccgct actgcgtgaa aagttgactt gtacgaagtt taccttccat aagatcattt
    19021 gtcaatgaaa aagaaaatat tttttcattt ttctgaaaat tatttttata actttcacac
    19081 acaagtttat tttttactag aaagtagcgc cctcttgttt ttttcttgct actatttctt
    19141 gatgttcttg cttgattctt tctaatagct ttggctcttt caacagatca agagctgtat
    19201 tggcgagtag ttctgctgca atcgcaatag aagcaaggcc tttgtcactt ttagcagcag
    19261 ctttgaattc ttctgtgtgt ccggcaatat atgtctcaga gattgctacc gtaggctgga
    19321 ttgttggtat gacttggctg acatttccta catcagaaga accgagactt gttctttgtt
    19381 ccgtttctat ttcttcatct ggaacacctg cttcttttgc atgctggaag aataagtcat
    19441 caaaagaagg agtaacgatg acatcatcaa ctgcattttg gaacaatcca aactcaaaag
    19501 tcgtgcccgt acttatcgca gcacctcgta cgatattttc cactttttca tatactttat
    19561 ctaatgtttg ccgatttcct gcacgtaaat agaaacgtcc agcagcgtaa tctggaatga
    19621 cgttggcagc tactcctccg tctgtaatga tcccatggat attgacatct tttggcaatt
    19681 gcaagcgtag agcgttgatt ccattaaaca cttggatcaa tgcttccaaa gcatttactc
    19741 ctttttcagg tgctgctgct gcatgggaag cgacaccgta aaatttgatg tcgactggat
    19801 cattcgctaa agattctgtc gttttcccat aacgaaaagc tggatggacg cataacgcta
    19861 catcgacatc ctcaaaaaaa ccttctcgaa caaagctgcc tttagctgat ccgttctcgc
    19921 ccccttcttc ccctggggtg ccatatacac ggatttctcc accagtttca tcaatgaatt
    19981 gcttcactat gctggcggct aatacggagt aagtaccaaa caaattatgc ccgcatgcat
    20041 gaccaatacc tggtaaagca tcgaattctg ccaaaaaggc aattgtggga ccgggtttat
    20101 tggaaatata acgtgcatca aaggctgttc ggtgtccagc tacttctttt tctacccaaa
    20161 acctttcttt ttctaattgt ttaatcaacg ctgctgagga ataaaactca tagttactta
    20221 cttcaggatg ttcgtggata tctagagcaa gtgcttgata ttctggtaaa cgatattgga
    20281 ttagatcctt caattggttt gttgagattg ttgttgtcat aaaaaattcc tcctatgcta
    20341 gtcatttttc ttattttctc ccgaatgttt cccatgcagg cacgcttgat ccttttgatg
    20401 ttttctcgat tactttgact gtgtcttctg tttgataagc gtctactatt ttttgatacg
    20461 ttttattgtt ttcatcttct tttcttgcaa cgatgatatt cacataagga cgtgcatggt
    20521 catccactgg ttctaagaag atggcatcag aagcaggatt aaatccagca tctacagcta
    20581 caccgctatt gatcactgaa atgtctacat ctgacaaagc acgtgctgtt tgggaagcat
    20641 ctaattcaga aatgtctaga ttttttctat tctcggtaat atcagaaaca gtgggcgctt
    20701 gtccttttgc cttatccact ttgatcaatc cagcagtttg caataacaga agagctctgc
    20761 cgccatttgt aacatcatta gggattgcta cttttgctcc gtctttgata tcagctacat
    20821 ccttcaaagt gttagaataa attcccagcg gtgcattgac agtatttccg atcgatacta
    20881 aatcagttcc gtgttcttta ttgtaattgt ctaaaaagaa ttgatgttgg aaagaattca
    20941 aatcaatcga tccatctgct aaagccacat ttggttgatt gtaatctgta aatttcacgt
    21001 attccaaatt aatgttctcc tttttcaaac ggtctttcac gttgtcccat acatctgtat
    21061 cttctccaat aatccctagt tttacgatag tttctttttt gtcagttgta tctgcttttc
    21121 cttggctaca ccctgccaac caaataattg ctacaaatgt aatgactaca aaacgaataa
    21181 tttttttcat tttttctcct ccactttcaa tgtgtaattt ttttaatcag ttgattactg
    21241 ataaattgac ttaaaaaaac taagattaga atcaataatg tagccacaat cgttacatct
    21301 gtttggaatc gattataccc tctcgatata gcaagattcc ctaatccgcc tgcacctact
    21361 gctccagcca tggtagtcag tccgatcacg ttgatgatag tcaacgccga gacccgaatg
    21421 ataccaggca aaccttctaa caaatacaca cgtaaaatga taccgattgg actggttccc
    21481 atcgcttctg ccgcttcgat caccccagga tcaacttcta gcagtgcgtt ctcgatttga
    21541 cgagcaaaaa acggaacaac tccaaaaatc aatggcacta aagctgcttt cgcaccaatc
    21601 gtcgttccaa ctaagaaacg agtagtcaat gctaaaagag aaagcaagat aatgaaaggg
    21661 atggaacgaa tgatattgat cagcttatca agtatttcaa atagcagacg gttagctagt
    21721 aatccattag ggcgtgtcac tactaacgtc acacctaaga tgattccaat catcccgcca
    21781 atgaatgctg tccaaaaaga catatatagt gtttgcttcg tcgcttcgac aaattcatcc
    21841 ggaatcaaat aaacattggg caaatatgtt ttaaagaaat cagtcatttt atcttcctcc
    21901 ttcgatcact ttgatacgac tatctaaatc gtttttttgt aaaatattta tttttacacc
    21961 agatttgatc aaataatcca atgctttttt ccgctgtttg gtttctccag ctaaagtaac
    22021 gatcaaactc cctaacggcg tttcttgtaa taactctaca ttgccatata aaatattcgc
    22081 tgttacttga tatttgctgt acagatgagc gattagaggt tcgttcgtct gatttccaat
    22141 ataacttaat tccaccagcc atttattggc gatctgatcg gcaaaagcag aatgggcaat
    22201 gatcgtttct aatgcttggt caagatgagt agctgttcga ataaaatctt tggtcaattc
    22261 ttcttcagga ttgctgaaga tctggatgct gctacctttt tcaacgattc ttccatcttc
    22321 cataaccgct accttgttgc agatttcttt gacaacttgc atctcatgag tgatcaggac
    22381 gattgttaaa cctaactggc gattcaattt ttttaacaat gcaaggatct gaagggtcgt
    22441 tttcggatca agtgcactgg ttgcttcatc acataataat accttaggtt cattcgccaa
    22501 agctcgtgcg attgctactc gttgtttttg tccccccgac agttgtttag gataagcaga
    22561 tgctttttct tccaaaccga caagttccag tagttcattc actttttgac gacgttcctg
    22621 tttcgatttt ccagaatact ttaaagaaaa atcaacgttg tcaaaaatag ttcggctttc
    22681 cattagattg aaatgctgaa agatcatacc gatggccttt ctttcttttc gcaattcttt
    22741 gggagagagc tgactcagat tcgtctgatt gacaatgact tctcctgctg ttggtttctg
    22801 cagcaaattc atcacacgaa ctaacgtact tttccctgca cctgagtaac cgacgattcc
    22861 ataaacatcg cctttttcaa tcgttaaatc tacagaatct actgcctgta tctctttttc
    22921 tttttgtttg aaacatacag aaacatctcg caattcaatc attaaaattc ctccttatct
    22981 aaaaaaagcg tcctcaagaa aatgaaaatg tcattttctt gaggacgctt ttttgcgtgt
    23041 taccacctca tgttcaagct gtgttcacac acaactcctc ttcaagtacg atcatactct
    23101 agcaagatat cggtcgcagc cgtcatcgcc ttacatctat gctcgggatg aattgctcga
    23161 tggccatttt ccaatagtcg ttccttgctt cttctcagct accgaagctc tctgtcaaag
    23221 gttcttctat cgtacttacc atttcaatgc aaataatatt caaaaaaaaa gtccttttac
    23281 taacataaac tatgtcagta aaaggacgac gtatcgcggt accacctttt ttcggaagtt
    23341 tccttccctc aagcccaaca tctcaaaaag agagattggc gttcgtttta acggtcgaac
    23401 agttccgaat ttgcctactt atcgacaaat tattctccaa ggccattttc agcttttctt
    23461 acttgactct tctcaccagc cagagcctct ctgcaaagtt tccaaactta ctttcctttt
    23521 caacgaaatc gatattttgt tgttgggatt aatctacatg aggaaaactg aaaagtcaag
    23581 tacgaaattc aaattatttt ttctatattt atttttctgt ttttttattt aacatttcaa
    23641 taacagttcg aagatctttt ccttccgcaa gaagtttcaa tttttccttt tcttcgacgg
    23701 tataatttcg gacaaaactg gaatcaggta tcaaaacaac gcctaatcct gctcgtgaag
    23761 cagctgttgc tcctactaaa gaatcttcca ctgcaaaggc tgaactagct tctaatcctg
    23821 ttcgttccaa agctgtcaga taaggaatcg gtgaaggctt gttctccgac acttcgtctc
    23881 cgaatgtaaa cgtgtccaag taatcagcta atttaaagtg atccaagata tctgttgctc
    23941 ttttcgtaac agtggtcgta gcaatgccta gtttcatatg acgttctttt aagtctgcca
    24001 atatttcttt tgcatatggt tttaagtgga tggctccgct tgtcagctgt tgataaaaat
    24061 attcttcacg cttcgctcgg acttcctgta ctttttcagg gctacctgct gctttcacta
    24121 aaatatcaaa actttgtttc cagttttttc cgccccaagc gtcaacgatt tcttttggga
    24181 tatggatatc aaattgctga aatccccaaa gccagccatc acgatacgtt ttttctgtgt
    24241 caactaacaa accatctaaa tcaaatattg cacctttcat ctatacatta atcctttcca
    24301 aaacaataca catagtttag cacgactggt ccaaaaagaa cagaagcaaa gaaccagcct
    24361 tctcgtttat aagagttctt ggaatcgacg gatatagatt ttcttcacag ctgtcacaag
    24421 cgccagataa gcgatgactg tagcaaataa ccataaccag taattgccag gaagtggcat
    24481 caagcctaat tcagcaccaa aacttgtaaa aggaaggaca gaaccgacaa taattcctaa
    24541 tgtagtgata acaaacatgg caaatgttgc gttgctttgg ataaatggta ctttcggtgt
    24601 acgcagtgca tggagcacta aggtttgtgt ccatagtgat tcgacaaacc atcctgcatg
    24661 gaataatgcg atgaaaatca ctcgctgtga actatccaat gtatggaaac ttccgcccac
    24721 tacagcagga caaataacaa aatacatcaa caagtaagta gtaatatcaa atatcgaact
    24781 tgttggacca aagtaaacca tgaattcccc aatactagat gcattccatt tttttggctc
    24841 gtccaaatat tctttatcca tccgatccca tggcaaggaa atgcaggaaa catcatagat
    24901 cagattcaaa aacaaaagtt gtaaaggcag cattggtaaa aatggcagga acgtacttgc
    24961 aatcagcacg gaaaacatat tgccaaaatt agaacttgct gtagccttga tatatttcat
    25021 gatattccca aaagtttctc gtccagaaag aaggcctctt tccaaaatcg tcaaatcttt
    25081 ttctaaaagg atcacgtctg cagattcttt cgcaatatcc acagctgtat ctacagaaat
    25141 accgatatca gccactttca ttgcaggagc gtcattgata ccatctccta aaaatccgac
    25201 agtatgacca tttttacgta aagctgtcgt caaacgtgct ttttgctgag gattcaattt
    25261 tacgaatact tcatggtctt cagctacttg tgctaattca tgatcactca tttccgagat
    25321 tttctcaccg gtaaccaatt cattgatctc tagacctact tgacggcaca ctgaacgtgt
    25381 aactaattcg ttgtccccag tcagaacttt tacagctaca ccatgttctt tcagagcttt
    25441 caacgcttgt ttcgttgttt cttttggtgg atcaaggaaa gcaagataac caatcaaaac
    25501 catgtctgac tcatctttga ctgaaaattc tccaacgacg cttggattgg tcttttgtgc
    25561 gacaccaatc actcgtaagc catcttcatt aagtgactgt acttttgcaa gtacggcttc
    25621 ttttcgttct tttgttaatg gtgtaacagt accgccaaga tcaataaatt tagaaatgtt
    25681 cagcatttct tcaatcgcgc cttttgtgat catttgtgtt ttaccagctg gatcagcaac
    25741 cacaacactc atacgacgac gttcaaaatc aaaaggaatt tcatccactt tttgataatt
    25801 gatcttatct gtatctaaag tttctgttgc ttcatcaatg atagccacat ctaacaaatt
    25861 tttcagccct gtttgatagt agctatttag atacgcatgg cgcaaaacac gatcatcttc
    25921 atttccttca caatccatat gatattcaag aatgatttta tcttgtgtca atgtccccgt
    25981 tttatccgta cataacacat cgattgcacc aaagttctgg atggcattca aatttttgat
    26041 cactgttcct tttttggcca tcacacttgc tcctttgacc aaattcgtcg taacgatcat
    26101 tggaagcatt tcaggtgtta gaccaactgc tacagatagt ccaaaaagaa aagcttctag
    26161 ccaatcccct tttgtaagcc cattgatgac gataactgct ggagccatca atgacataaa
    26221 tttgatcagt agcatggatg ttttatggat gcctaattca aagcttgatt gaattggttt
    26281 ttcagatagc gtttctgcta catgaccaaa tagtgtttca tttccaacag aaacaatgat
    26341 tccttccgca cttccactga tgacattact tcccataaag acaagatttt cgtaacttgt
    26401 ttcgctttgc tgatcgtctt cacatacttt cgcatgcttt tctactggat agctttcccc
    26461 tgtcaatgct gcttgggaaa tgaataaatc ctttgcggac aataaacgca tatccgctgg
    26521 tatcatatcc cctgctgaaa gccgaacaag atctccacag acgatttctt ccataggaat
    26581 ctctgtgtac tttccattac gttttacagc tgccgttact ttcacaagtg atttcagttt
    26641 ttcagccgct tgattggaac gtacggactg aatcaacgtc attgtcccgc tgacgataac
    26701 catagtgaag ataatggcta caccgaaaag atctttatct ccaggagctg ctaatacata
    26761 ttccgtgatg aatgaaataa ttcctaatgc gatcaacact aatgtaaatg gcgtgatgta
    26821 tgctttcaat atttctacaa tgaatggtgt ttttttgcca tatgaaatcg cattttcacc
    26881 gtaattttct cttgaacgtt cgatttgttc ttctgtcaat ccatttgctg atgcatggaa
    26941 tagttcaaat aattcttgtt tcgtttttcc agcaaaagct gcatagattt tttgtgtatc
    27001 tttctttttt tccatctcaa tctctccttt gtgtccaaaa gacagctgtt ctcgctgctt
    27061 ttggtcttta gctattcatc ggatcgatag agaaaaaact gaaaggggct gagaacagat
    27121 agataatagc cctggccttt cctctagatc cttgtttttc gaatgactgt catccatttg
    27181 ttctcatctc ctttactaag taattttaat tcatgacttc caatcatttt tcgaatagtt
    27241 atttttccaa ttagaccacc tcctttttat ttttcaccga tggggatttg cggcaacaaa
    27301 aaaacctctc gccaaatact cagcgaaagg tttgaaaaag catattaaaa aaagcttctc
    27361 agatctcatc gttgagtttt gacactgtac aacgtaaaaa gcagatacct gcttttagct
    27421 accttatgaa aagccttatc tcgacaattc ctgttctacc cattggcgtc tctcgacgtt
    27481 tttgggcagt agcctatgtt tgtataggag cctcacctaa caaatcttat tcggttttcc
    27541 ttcgttaata taacaactaa aaaagaggat gtcaaatcaa ttttccatgt ttttcatact
    27601 ttttttaaaa tacacacgtt atattcctgt ctcacgcggt tttatttgtc tcattttcca
    27661 cctcccgcta taatagaaat atagtaggaa acgaggtgct gttatggaaa gaacacttgt
    27721 tattatcaag ccagacggcg tgagacgcca tttagtcgga agcatcatcc aacgttttga
    27781 agcaaaggga ctggccattg ctgaaatgaa gtttgaaact atgactcctg aattagcaaa
    27841 agaacattat cagcacttga ctgagcgcag tttttttgat gaactgatcg attacatgac
    27901 ttccggtcct gtcgtttatc ttgtgctggt cggcgaggaa gtgatcgata tcgtccgtaa
    27961 gatggttgga gcaacaaaag ctgccgatgc tgttccaggt acgatccgag gagactacgc
    28021 gctgccgggg actgaaaaca tcattcacgc ttctgattcc cgtgatgctg cggtaaaaga
    28081 gatcgcccgc tttttcccgc cattcgatat aaacaaaccg tctgaaaaag cggcgaagtg
    28141 atacaaattc taaactgctg gtctagttgc tgggttcagc aatggccagc agttttttct
    28201 ctaaggacct ccgttgattc tttcctaaca tatattcgag cactgtgaag aattcttggt
    28261 agtctttcgt cttttcttcc cctaaagcag cagaaatcaa tctttgataa taatctgcca
    28321 tcaatgtttc atagatcact gtacttcttt ctttatgtgc taagctccgt ttcatatctt
    28381 cattcacact acgtgccttt ttcaaccgac cattttctat ataaaacgaa gtcaggttca
    28441 ttaaaaaagt aaagagatac tgctcatgat tgttcaactg tgctaatcgt cgcattcttt
    28501 ttttgatttt caaataattg ttctcaatga accgatcact gaatatccaa ggaaaatcag
    28561 ctagaataga tagttcaaat tgaccatagt ctggtatctg catcaagtat tcttcaatga
    28621 ttcggcattc ataacgtaaa aattcaaatt ctgtctcaga tacttctgtt ttttcgacca
    28681 tgtgaagatt caaaaccaaa taatacagat cttctgtttt cttaaaatgc ctacggttat
    28741 tgctgatctc tttataagga tcaaagctgt cttcttgtac ccgatctagc attcgtcttt
    28801 ttttctttaa ctttattggt tcatccagct ggtttcgcat ccattcatat tctgcaagtg
    28861 tcatgttcat tcgctctaaa aatgatctca atgttacaaa tgacattttt ttcactgaat
    28921 ttgtctcaat cccgactaga gaagtcttaa aacagattcc ttccgctaat tgggactgtg
    28981 tcaacttctt gttgactcga agtttcttga tcaattcacc atcatacata atagttctcc
    29041 tttttaattt aaatttttcc acattcaata aatagatacg ttaaaaagga aaaatacacg
    29101 aaaaaaataa aaaaatgttt ttttcataaa aaataccaac cttcaagcat tagaatgttg
    29161 tctaactact gaaggttggt ttgattgttt attgattatt tttttgctgt tcaacgtctg
    29221 ttcttgttac tggatataag ttggttgttg ttttattacc attttttgaa aagacacttg
    29281 tcgaatgttc gcctttttgc ttttcgattg cttcattacg ttccgcttca tttttatagt
    29341 catatttttt cggatcaatc gtctcaaatc cgtttggcac ataaaaccgc aagagatttt
    29401 cctgattcag cttatctgaa agacttaacg cagtcttgac tttttcctga tctttggaga
    29461 tttcctcatc gattcctgct gctttcgtat cgatgatttc tcctgtctga ttattgtaag
    29521 cttttcctcc taggacggtg tatttgggtg tcacaaagtt cccatttcga aaagcaacta
    29581 cttgatcatg ctgagaagat aataagtctg taccaaaatg aagataatct ttatcatcga
    29641 ttcccaatag atgtagtaat gtcggcaaaa catcgatttc tccaccgtat tcatgattga
    29701 ctgtcccttt agtataacca ggcatatgga tcatcaatgg cactcgttgc atctgtgcgt
    29761 tgtcaaattc atcccatgta tctggatctt taccaagagc gcttgctaaa tctttattat
    29821 ctgtattcga gatcccaaaa tgatccccgt aaatcacaaa gattgaattt tggtaaatac
    29881 cagatttttt caaatacgta aaaaattgtt ccaatgattg atccagataa tgtgccgttc
    29941 gaacataatt atctactgtg ctattgccag tattcaaaga tggaaagtcg aaattcttgt
    30001 catccgtata ataaggcgtg tggttggtta cggaaagaaa tttcgtgtaa aacggctgtt
    30061 gtagatgctc aagatattgg gcagattctc tgaataaatc tttatctaat atcccataac
    30121 ctaacgtttc gtcggattca tcaaaataag atcgatcaaa gaaattgtca taacctaagt
    30181 ttttatatac atgatctcta ttccaaaaac tagctacatt cccatggaaa acagcgcttg
    30241 tatagccttg cttttgtcca aggatcgctg gtgcggcttg aaagacattg tctgacccaa
    30301 gttctgtgaa taatgagcct tgaggtaaac caaatgtgcc agtttccaac atattttcag
    30361 catcacttgt ctttccctgt cctacttcat gaaagaaatt gtcaaagctg atcgttgctt
    30421 gattctggaa aatactattt aaaaacggtg tgacttcttg tccgtctacc ttcatattaa
    30481 tcaaaaactg ctggaaactt tctaaatgga gaacgatcac atttttccct ttagcgatcc
    30541 caaatgtttc gggatttggc tctgcataat gtttctttgt ataatctaaa acttcatcaa
    30601 tcccattact gcttgcatgc gcgcgaacac tgctagtcat tcctgttttg attccgtcat
    30661 aaatagtata cgcatcgatt cctagatatt tgacaatata tgaacggtca aatgttcgct
    30721 gcagcaattg tggacggttg gcttcgctca gcgctagatt tcctgaaaaa ataagccctg
    30781 aaagacaggt aacagcgata gccatcggtt tgccgacagg gttgcttttg ataggtattt
    30841 tcttgacctt caaccaaacc agtagcccaa tgaacacagc gatatccagc cagtagatga
    30901 catcatgggg cttcatcaaa gaaaaggagc ttcctgacaa tccttgcgac actttcgaaa
    30961 agcctaatac agatttgact gtaatgaaat ctgtgaattc ccgataaaaa ataatattca
    31021 agtacaacaa aagcgtattt aaaatatcaa tgatcaaaag aatagctaac gctggctttg
    31081 gctttttaat atacaaagaa aggccaaaaa acaataaggt agtcgcaatt gggttgatcc
    31141 acaagataaa gtactggatc gtaccttcca cacctaatga aaaatctgca tagtaggcaa
    31201 tgatcgtctt cagccagaaa aagaaaacag ctgtcaaaac gattgctaaa cgattttggc
    31261 acaatttttt aagttgtgtc acgtatatca cttctctcta ttcacatttt ttcttatttt
    31321 gctcctgaaa aatgaaaatt tcctaaaaaa gttgttatct taagattttt ttattctttc
    31381 taaagattat tccgtccact tttcacagca taaaaccgct gcgatgtaag cgattaaact
    31441 aatgaagaga taaaagtctt gtggtgaccg cttcactcca cggtattcaa aagccagttt
    31501 ctcggtattc gtacaaaaaa agaggctgtg acaaaattct tgtcgcagct ccttatttct
    31561 tgatgcagga cagctttttc acaacttctt ccttaaacgg tgttctcctg ccaactcctc
    31621 ggccacaagt cacaatattt ataaaacatg agaagctgtt ttttgaaatt gttccttgtg
    31681 gctcagagtt aaacggcagg ctcaagacct cttgatacac ggtgttcaat cagctgaccc
    31741 tcggtattag attatgtcag gacgctaatt ttacaacctc ttggtaaacg gtgttcaaaa
    31801 gcttgcatct gcggtaataa gcccaaccaa acgaaaaata taaggagcta ttttttgaaa
    31861 ttgctccttg tggctcagag ttaaacggca ggctcaagac ctcttgatac acggtgttca
    31921 atcagctgac cttcggtatt agctcaaaaa acggcaaaat atgaggaact attttgccgt
    31981 ttgtttctct aatactcagg tcaaaacgct gatttcacaa cctctgtttt tcattgtcca
    32041 taggattgat attgtccttg tgatgactca ttcgtaataa ttgaggcttg attttcttca
    32101 cttcccgtta tcgtttgtcc actgccagaa tctgaaggac ttgttcctgt cgactcgttt
    32161 ttttgagaag agacagttgt tttcttgact tgttcatttg tctttttcca ttccgctaaa
    32221 cgatcggcga ccactttttt gtctactttt tcagaataag cggtgatcac ttttccattt
    32281 ttcaaacggg ctactttgtc gccatcatga gtcaaaagca aaacggtcaa atgcttgtct
    32341 ttaaattgat attgtctgag agaaatcgca ctaagtacgt catcgatatt aataaattga
    32401 ttttcttctt ttccttttgg caaataaatg tccaattcgc ctttttcgta attggcagtt
    32461 attttatact ccgcccacca ataagttgag aattcttttt tgaaattatc tactgattct
    32521 ttgcttgcta cgattttttc tgtttctttc tgagcaagat ctgctgtcat ttttcctgaa
    32581 aatgctgcgg ataaaataag cagtaagaaa aaaccgaata accaaaagag aagaaacggt
    32641 ctttttttcc tcatgctttc ctccactcct atttaatttt gactattcta ttatatacaa
    32701 aaaatgaaat agaaagacaa aaaagttgaa aaagatcgta aataattgaa cttctttttc
    32761 acaaaatggt aagaatggag aatttcacgc atttgctctc aactattctt tcttaagcct
    32821 caaatccgtt tttatctgat acgtctttga accaatgacc cgattttttg attgttcttt
    32881 ttccatcttg tgctaagtct actgaaataa agccatatcg attcttatat gcattggtcc
    32941 acgaccagtt atccatacat gtccacatat gatagcctaa acaattggca ccttcttgaa
    33001 tggcttgatg gacatatttc agatgagatt ggacaaattc gatccgataa tcatcgtgga
    33061 tcatgccatc ttctttgata aatcgtgctt cgttttctac acccataccg ttttcagaaa
    33121 taaagcagtc gatatttcca taatgatctc gtacattgat caaaatatca tagattcctt
    33181 gttcatagat ctcccatccg cgataaggat tcattttacg accaggcatt tcataattgt
    33241 cgaaataatc ctcaggcatt ggaccgtttt tagtatcgat tggtgtttct tttgctttta
    33301 cccggcgtgg ctggtaataa ttgataccta aaagatctac tgtattttct ttgataaccg
    33361 tcaaatcctc tgcatggatc acaggcatga gatcaagttc ttttactatc tcaatcaatt
    33421 cacttggaaa ctcgcctttt acagctggat caaggaatga acgattgaag aacgcatctg
    33481 ctatttgcgc tgctttaaca tcttcagggt ttgattcgtc tcttggatag cttggtgtca
    33541 gattcaagat gataccaata acaccacctt gctccatttc atggtaagat ttgatggcta
    33601 acgctgaagc caacgcttca tgatagccaa cttggatcgc ttttttcata tctgactctt
    33661 ctggataatg ccatcgatat aaataacctg cttcgactgg aacgattggt tcattatgag
    33721 taaaccattt ttttactttc gaaccaaaca attcaaaaca acttttggca tatttttcat
    33781 aggcttcaac cgtttctcgg ttcaaccatc cgcctttttc ttgcagatac atcggcatat
    33841 cgaaatgata cagattcaca aagggttcga taccgttttc aatcaactca tcaaaataac
    33901 gatgatagaa acgaacggct tcttcgttga ttgttccaga ttcatcaggt atcaaacggc
    33961 tccattggat agatgttcga aatgaattat gcccagtctg tttcatcaac tgtacatcct
    34021 cttgatactt cgtataaact tgcgaggttt tttcaggtcc tacttgatca aagaactttt
    34081 gcggttcttt tttgtaccaa taatcaaaaa tgttctctcc ttttccatcg cctgaaatcc
    34141 ttccttctgt ttgtgggcca cttgctgcag aaccccacca aaaatttgct ggaaattgat
    34201 attttttcat caaagcacct ctttttcttt tttagttcgt tttagttgat tccatgtatg
    34261 atattttcca tcacaatcac tttttcattt tcttcctctt cttttttgat tcgttttaac
    34321 agctgttctg ctgctcgttc cccgatttta tctgttggtg gcgtgatcgt gattttcgga
    34381 aggatactca gatcgttttt atctaaataa tcatagtgac tgaacgcaat atccttcccg
    34441 attctgacac cttcttgatt gaaacattcg atggcaccaa tcgacatatt gtaattgctg
    34501 acaaaaatag cagttgcccc tttttctaat aatgcttttg ccccgtaata gccgcttttt
    34561 ctagaatagt ctcctgtaaa gacctctaat tggatcgatg gatcattttg gaccgcttct
    34621 ttggctcctg ctaaacgttc tcttgctacg tagtctgttt gagtggcagc aataaatccg
    34681 atttttttat gtccttgttc accatgatga gcgatcactt ttgctacact ttctcgatca
    34741 ttcactaata ttgtatcaat acctgctggt ccttctggac aattcaatgc gataactgga
    34801 atactcattt ttgtgagcat ttcaaatcca gaccactctt cttcggataa gaagacaatc
    34861 agcccatcga tcacatgctt catcaggtat tcaatttttt gttcaatttt ttctggctga
    34921 tcggcaaacc cgcttaataa taagctatac ccattttctt cagctactct ttcgatccct
    34981 gagacaactt ccgacccaaa acggctggaa atcccgttta tcaaaagtcc aatagagaag
    35041 cttcgattat tcttcaaccc tttagcaatg atgttttctt tatagtccaa cgctgaaatc
    35101 gcttctgcta tttttttcat gttcttttcc ttcagttgct gaccattcaa gtaacgggaa
    35161 actgttccca ctgaaacacc agccatctta gccacatcac ggatcgatgc catacattct
    35221 cactcctgcc agttacattt gatttctctt tcttgttgtt cgattatgat gaccgcattc
    35281 tttgcatagt gttgcttgtc atcaatatcc atataactaa agtcttcagt agagcaactt
    35341 cctctcacta ttaattatac ttaaacaagt atttgaaccg gttcattttt aatatagcat
    35401 ttttttaaaa gattgcaaca cttttttaaa acgctttctg aaagtggcgg attttttgcc
    35461 tttttaaaat tttattctaa aatttactct atttgtgggt gttttgtgcg tttttctctt
    35521 ccatccgttt gaagttttac attccaatgg tataatttct ttatcctatc aaatggggga
    35581 agtattttga agaaattaat cgtactattt attttgtctg cgggactttt gacattgact
    35641 ggctgtgatt tgtttggcgg ctctgaccaa tcctcggacg aaacaaccga ttcttctgtc
    35701 ttaaaagcgg aaaaagcaga taccaccaac agtgtatata cgctaaaagt acgtaaagat
    35761 atggatttat ccgcgggaca gttgacattt acagataaaa aaatggaatg gacacgcacg
    35821 tatcagacac aaggaactga ttcttctgaa tcatctgaag aaaaaacagt gctgacagat
    35881 atcaagatca ctaccaaaga tgacgaatat attatttcag gaaaagaaaa taaaaaagaa
    35941 acaacagtca tatttacaaa aatcggtaat taccgtttga aggatgagga tggcaacatc
    36001 tacagtctat agtgccgtct gttggatctt cttttgtttc tccccttctt ttctgctata
    36061 gtaaagaagc aagatgattg taaaggagac gaaatggatg atcgatagtc ctgattattg
    36121 tacatgtaat aacaacaaaa atatccatac agaatttaat gaccaaggaa gcttgagcat
    36181 atgcgacaac tgtcacaaac tagtcaaaga ttcatttaaa acatttgaat cttatcctgt
    36241 tcaaaattat gtaaaaagta gaagaagata aacaaatcca agcaaaactc actatggctg
    36301 atttttatga acttcaaact cagcttcttc ctttgatcaa gcttcatcag taaaacaaac
    36361 agtggatcat ttcagtttac aaatcaaaga gtaaagattt atttatttca aatatttgaa
    36421 tataaaataa ccaaacgata gaaaggctta ttctacatca cgtagtaatt tttctttccc
    36481 gtcgtttggt tttttatttt tagcctaatt cttgaccttt tgcatgctgg ttatccgtaa
    36541 aatattttcc agcaggcttt ccttccaatt cagctaattt ttcttggatc tgacggatca
    36601 ccttttttgc atcttctgga ttttctacaa aatcgaattt atccccatcc acttgtattt
    36661 taggacatac atcaaaatct tcataccatt gattatagcg ggtgttcaac gtttcataat
    36721 aggtatataa cgtctcatcg ctttctagct gttcatagtc acgcccacgt tttttgatac
    36781 gttctagcat cgtgtccaaa gaaactttga tatggaccat caaatcaggc cgtttttttg
    36841 gagcaacatc atctaactcg tttagcatcg tatctaataa gctatcgtac tgctgtactt
    36901 cgatatccgt tacccgacct aaatcagcgt ttagatggaa caacaggcta tcttcataga
    36961 tggatcggtc tagcacattg tcatcgttca ctaaagcatt tttcattgct aaaaatcgct
    37021 tgttcaaaaa gaagatttgc aataaaaaag cgtactgctc aggatttgaa taaaagagcg
    37081 gcaatacttc gttatcttcg acattttcat aaaatggttt agaatcaatc tcttttgcaa
    37141 gcatatccgt caaactgctc ttacctgcac caatcgttcc tgaaatcaaa atcactgaca
    37201 tacaaccctt cctcttctat aagtactcgc ctacgtaagt ttcaacaggc aatgtttcaa
    37261 aatcatattc acgtacagct tggtctgatt ttaaatacgt atggagactt tgtgtaatca
    37321 tcaagttcgt aggtaccgtc tcttcttgta caatgatcat cggttttttc aacatagttg
    37381 ccacacctaa ttcgtaagcc gttcctggat cgattgtttg atgctcaaag tcaaggatag
    37441 caacaacaaa ttctgcattt gtcagttctt ccatatcttt ttcgtatact tcctgcgccc
    37501 atccagcgct gaatagttca taattgcttt cttggtgatg acgtggagag tagaaacttg
    37561 tgactgtttt gttttcctcc aacgcttttt cgatacgact tactcgatcg atttgctcct
    37621 ctgaaaaaaa tggtcctgct aaatatattt gactcattat tttgcctcct tcaataataa
    37681 ctgattttta atagatactg ctcgtaattc attgaccagt tccaatggat tgttggaaaa
    37741 gattacttga tccaaccatt ttttatccat aaaatcctct tttgcaaaag tttctaataa
    37801 ttgttgcaag ccgctgtaat aaccgccaat attcaatatt ccgattggct tgtcgggcgt
    37861 cagaccgatt gctatccaag aaagaagttg tgagaattcc tccaatgtac ctagacctcc
    37921 agggaaaacg aggacagcat ctcccttttc aaataataat acctttcttt catcgatcgt
    37981 ttctgtagga ataaatgtag taacatcttt tccaggcaat tcagtttcaa aaagcccatt
    38041 cggatagata cctgtgactt ttccgttatt ttccagtact gttttactaa tcactcccat
    38101 aatcccggag tatgaaccac cgtaaaccag ttcgatttct tcttgtgcca tgtattttcc
    38161 taacgtttgc gcaataaatt tgtaactttc tttattacca aaacgggaac cgcaaaaaac
    38221 agtaagtttc ataaaaaaac actccctaac tcaaaagtct atcttcattc tacacgtgtt
    38281 tttttaaaat aaaagactag attttgtagt tttttagtgg tatataggat agacgccact
    38341 atatatagta tttagaaaaa aatcattttt cagccatttt atagataaaa caaaaataaa
    38401 taacgtgttt tcatcgatat gaaatgactt acttatagaa gatactggga tttcgtccta
    38461 tatgccaata acgtgtatta ttttaaacaa caaatacttg tttcttttat taacgaaaaa
    38521 atcattttgt tctcgtgtta tgtttcactg ataaatgatg tttctctaaa taaaaaagcc
    38581 gacgaactaa acgatcaaaa tttgatcatt tttgttcatc agcttctcct aaaaatcttt
    38641 ttgttaacga taaatagctg gatttgtaaa aatatcactt gaatcgtcac tgctggaaga
    38701 aaattcagtg ataaccgcac ctgttgcgcc tgcctggaac tagtgtttga tattgggctc
    38761 gaacgtgtac tgatctctag catgaagaat aatttctttt tttactgtat aataactttc
    38821 tttccttttt ggaatagtac aggaaagttt ttttgtttcc tttccttcga cataaggtat
    38881 accagaccct ttcgacaacg aaaggttctc atttttcctt catcgtcatt tcaaggaggg
    38941 tgccgatgct ctggacagtt tcgatcgctc ctaaaccaaa atctgtatat tcgattcttt
    39001 ctaactctga ttctttcata ccattcctgt ctcaaggtac atctattgta cttttttctt
    39061 gtaatccatt ctgcctctcc taactaattg tcaaatcatc ccaagatatg tcttcttcct
    39121 ctttttcttc ttggatgatt tctgcattcg gatctactgg ttttttcaat aataaaatgg
    39181 ttattcctgt gacaaaagaa ccagctaaca atgcaccaat gaatgcccat ggtctggtca
    39241 tcgtagcgac tgcaaacatt cccgatgccg gaatacggct atctgcgccc caaaacatgc
    39301 ttaatccgcc acctactgtc ccaccgactc ccgttgctat gacattgcgt gccaagtcgt
    39361 tcaatacgat agggaaacat ccctctgtga tctgacagat ccccatagga aaagcggtct
    39421 tcaatgtatc gatttcttga cgattgagta tatttttacg aatgattttc cctaagaaat
    39481 aagcgatcgt caagccgaat ggggttacca tcgaagctaa aatcaataca gtgattggtt
    39541 catagattcc ttctgaaaat cgtgccacac ccccttatcc agaataaatg gtaatccaga
    39601 aacagccgtg tatctgcggt aataagccta accaaacgaa aaatatgaga agctattttt
    39661 gtttggctgt tcttattact tgatgcaggg cagctttttc acaacctctt tttttattta
    39721 tgtttcataa tcaatatggt aaggatgcag ctccgtatgg tagaccgcct ccgaatattc
    39781 aatcatttcc ccaatttgcg tagacgattt ccgtatccgt ttgatgccga tctgtgcgtc
    39841 tgttttcaga attccttgtt cttcttctgt taaaaatact gccgagaaat catcagaaaa
    39901 tgagtcaata gtatatcctt ttttcgtcag caaacgatac aaagattctt tgccaaattc
    39961 ttctctgctg aattgcatca tttcttgtgg aagataatga acaaaataaa tataaggctg
    40021 attgttcaaa taatataagc gcttaaaaca aacagcttgc tcaccgaaaa aggaaaacaa
    40081 aggatcttcc ttctttatat ggaccaattg caaatccagc gtttctttcc ggacttcata
    40141 tcgggagttc tccaagattt gcgtaaagct tgccgctttg gataatcgat tgtaaggacg
    40201 attgcttaga accgtcgtcc cacgaccact tttctttgcg acatattcat ctgatgccag
    40261 cagttcgatt gcctttcgga cagtgatttt gctgacttga aacagttctt ctaattcact
    40321 ttctgtcggc aacatcgaac cgactggata ttttcctgag aaaatatctt ctttaatttt
    40381 atctgcgact tccaagtata gtacacgtgt ccttttcata acgtcaccct ttgctttact
    40441 attactaaaa gaatagcaca taaaaaataa aaaaagaaca caaatcacat gtgagaagtg
    40501 tgttcttttt cattcagctg ctttaattac aagtttacgc catttgaggc aattacttct
    40561 ttataccaat caaaagattt tttctttgat cgattcagtg tcccatttcc ttcatcgtct
    40621 agatccacat agataaagcc gtaacgttta ctcatttctc ctgttgatgc actgacaaga
    40681 tcgatacatc cccaaggcgt atagcccatc aaatctacac cgtctgctac tgcattagcc
    40741 atcgcttcga tatgacgacg taaataatcg atccgataat catcaataat cgtgccatcc
    40801 tcttccatat gatcaatggc tccaagaccg ttttcaacaa tgaataatgg tttctggtaa
    40861 cgattgtata attcattcaa tgcgattctc agcccttctg gatcgatctg ccagccccat
    40921 tcacttgcct caagaaacgg attttttact cctcccagca agttcccttc tgaagttgct
    40981 gcttgtggat cagtctcatt gattgccgta gacatataat aactaaatcc gatgtagtcc
    41041 actggatatt cttttagcaa ctggaagtct ttttccgttt gctctaattt ttccggatcg
    41101 actccatatt ttttatgggt acgcgctgtg tatgcagggt attctccacg tacttggaca
    41161 tccgtgcaga aaaaattgaa ttcttggttt tggatcattg ttgctgcctg attgactgga
    41221 ttggcatcga tgctgtaagt tgttgcatag ataatcatgc agccgatttg gatatccgaa
    41281 cgcaattcgt ggccgatttt tacggctttg ctacttgcaa cgaattgatt atgccatgct
    41341 tggaaaatat tttggtactc tcctgctcca ttgcttttta ctaatccttg acttaaagct
    41401 ggaaagtgaa aggccgaatt gatttcattg aaagtcatcc aatatttgac tttactgctg
    41461 tatctctcta atactgtctg agcaaatcgt tcatagaagt cgatcagttt tcgatttttc
    41521 cagccaccgt attcttttgc caaatgaaga ggcatttcgt aatgagaaat cgtgatcaca
    41581 ggttcaatat catattttaa gcactcatca atcagctgat cataaaattc taatcccgct
    41641 tcgttaggag taccttcgtc tccatttgga aaaattcgtg tccaggcaat cgaaaaacga
    41701 taacatttga atcccatttc cgcaaacaag gcaagatctt ctttgaaacg atcgtaatga
    41761 tcgattcctc gatgattcgg gtaaatatat ttttcttgat caatcgtcca gtcaaattct
    41821 tcactgccga taatcgcaaa gcgctgctta cctccaggca tagcatctgc aacggatagt
    41881 ccttttccgt ctcgatcata agccccttct aattgatttg gcagcagttg ctccacccca
    41941 taaaaatttt tctgaaaaac gtgattgcat cctgatttcc tccttatttt acaattgcca
    42001 gaacatcttc gtttgcaata atctcttttt ggtctaattc aagtatatcg gcaaatgcat
    42061 cagtattcgt aatgacaact ggagtgatta cgctatagcc agctgctcta attgcttcga
    42121 tgtcaaaacg aatcaataaa tctcctgctt tgacgagatc cccttgtttg actaagatct
    42181 caaagccatt cccatttaat tctactgtat ccatcccgat atgaatcaaa atctccgctc
    42241 cgtcagtcgt tgtgatccct acagcatgtc cagttggaaa aatagtcgtg atctctccgt
    42301 ctgcaggtgc aactagttct cctacttctg gatcaatagc gatacctttt cccatcacgc
    42361 cagaagaaaa aacttggtct ggtacttcac tcaatggcag tactttaccc gttaaaggac
    42421 tttttaaggt atgccgtgct gaagtaatcg gttttcctgc attcgcatgt ttgttttcta
    42481 attgattgtc ttgtgtttgt tcgtcaaatc ctaaaatcaa tgtacctacg aatgcaagaa
    42541 caaaagcaat tcctgtagct attactgcta ctgtcacatt tgactctaca ccatccaccg
    42601 tattaatgaa agtcggaatc gtcaataagc tgaccaaact gctggagaaa gcttttactc
    42661 cggaaaatcc gataatagct ccaccaatac ctccagaaat acaagcggca atgaatggtt
    42721 tcttcaaagg taatgtcaca ccatacacag ttggttcagt gattccaaat aacgaagtca
    42781 ctgtagaaga aatccctaat gctcgaagtt tcgtatcttt tgttcgcaat gctacagcta
    42841 aagcagcccc accttgtgcc aagatggctg gaagaagcat tggcatcaat acatcaaacc
    42901 catattgttc gatattcaaa aacatgatcg gtacgaatcc ccaatgcatg ccgaacatca
    42961 cgaatacttg ccataaactt cccatgatta gacctgctac gattggacta aatccataaa
    43021 tcgagttgaa gagaccgcct aaagcattac cggcaaccgt cccgattggt cctaaagcaa
    43081 gaaatgttaa tggaaccatc actactaaaa cgacaagcgg aactaaaatc atttgcagaa
    43141 attgcggaat cacttttttt acgaatggct ctaatttact ttgaacccat acggctagaa
    43201 tgattggtat gacagaagaa gtataaccag atggtgaaag aatcactggg atcccaaaga
    43261 aactgatagc ttctcccgca ccagcaactg ctgtgatagc tggatacaca agtgccatag
    43321 caattgctac tgctaaaaac tggttggctt tgaacttttt agctgctgta aaagcaagca
    43381 tgacaggtaa gaaggtaaag acaccatctg ctgcagcaaa caatatttga taagtaccgc
    43441 tatcagctgt cagccagccc atcaccgttg ccaatgataa gaatcctttc aataccccgg
    43501 cggctgccat cgcccctaga aaaggtgtaa atatactaga gataatatcg atcagacgat
    43561 tcaaaatatt tttggactct tcttttccat cctccgaaac tgcaagacct tcctcatcca
    43621 cgatcgattg ataaacatct gctacattac tgccaatcac tacttgatac tgaccgccac
    43681 tttgtacgac ttggatcacg tccgggtctt cttttaactt ttgcgtatct gctaaagatt
    43741 catcttttaa tctgaatcgc aaccgcgtag cacaatgtac taaacttttg acattctttt
    43801 gtcctcctac cgcttcccat acacgacgac caactgcttg attatccatt gttttttcct
    43861 tctttctttt ttatttaaaa aataaaaacc cagattgaaa aaaacagtca caaacattcg
    43921 tgaacctatt ttttcaatcc aggttttgcc tgccatacag taacaatcct tatgattgga
    43981 tgcttttttt ctcacttact actctttgaa tatgaatcgt taaataaatc tgctctgcga
    44041 tccccatttc ctgtttttgc gtttgtcgaa gatactcatt gatccgttcg acacaatgaa
    44101 acgcttttgg atactttcct tgaactaatg cgtagagaaa ttcatcttgc tcgtcagatc
    44161 cttccccttt gagataacgc tgggcaaaaa actgcagatg agtaatgata cgctgataat
    44221 tcaaagattc ttcatcgaac acgatgccga agaatttact gatgatactc agtatatctc
    44281 ttaccattgt ggtagcggtc atcgtcactt gcatgtcttc gtttccttgt tggctattca
    44341 caaaatgaaa ggcaataaaa ccggcttcat cctcagaaag ttccacaccc gtcttttgtt
    44401 tcacgaacaa aagagcctct ttcgctgctt gatattcttt cggataaaat ttttttgttt
    44461 caaataataa tggattaggc agttggatat tttcttttgc tcgtgtgatc gcataatgaa
    44521 gatgatcggt caatgtcaga taaatattag gcgaaaattc aacttttagt ttatcttcag
    44581 ccagttcgac tacttgtttg acccattcga tctcctctgc cggaatatca gccatcagct
    44641 gctggaattg gtttttagca aaagaatctc ttaaaacaaa ttctttttgg accgcttctg
    44701 gcgaaatctc gtccccaatc tttttttgaa aagctagtcc ttttcccata caaacgactt
    44761 cttccccgaa tccatttttc gaaatgacga cattgttatt caaaatttta tcaattttca
    44821 ttttgcttcg ccctctcaag aaaaataaaa aaacctagaa ttcgcctact gtttcttagc
    44881 tgaattctgg ttttgcctgc cgtacagtaa caatccattt cttattcaaa atcatcatac
    44941 catcgaattt ttatttatgc aagccttttc atataaaaaa taaaaaaact ggaacacaag
    45001 tctatctaga aaacaaccaa atcagagatg cacctctttg cgtttctctt tttttcagca
    45061 aattggtttt tccttatcga ttcatatcct ttggctgttt ttaatctgta acgacttatg
    45121 tcccagtatc ctaaaatgtt tttgtctcta gattgaaagc agatctcata tcatctgtcg
    45181 catcttttat cgatgtaaca ctttccagtt ctccttgtac aactagtcgc gtgacggcgt
    45241 ccatgtcgcc acaagtaatt agtgtgacaa tttcttttcc ctcgacttca ttcagtaatt
    45301 gggtatctgt aggagcaact ttttctttaa aaaatgtttt gtatgtatac acattttcta
    45361 aatctgtcaa atagatcttg gcacccatct ctaaattttc tagcggtgta aacagcaatt
    45421 cagggttcgt tgcacgatgg cttgctaagg cataatttcc ttttcccatt tcttgatctg
    45481 gagataatgt tcctgctccg taaagtagtg acgtattatc caagcctttg aaaaccggta
    45541 aattgattga aactgaaggg actgcgatac tagcaatcac tggtagatct gttcctctca
    45601 tttgcgacct catgacccct tctgtcgtca ttggttctac cgcatcaaag tcaaaactga
    45661 catctttatc gttattcttt ttcagatctt cttttgttac attcgctata gcatatttat
    45721 ctcctgtctc tctgacaaaa taatccttga tctgttcatt gaaaatcaaa gctagtccta
    45781 ctaataacaa gaggaacaag aggatattga ctagccatct ttttgctttt cctttttttt
    45841 tcattttctc tccctccaga ctcattttac tgtttttctt ttgctctttc caacagccga
    45901 atgacacatt tctacaactc tgtgtatttt tttcgtacac ctgcgctttt ttctacagga
    45961 tatttcaaat tatttttgat caatttttct tcaatgatct catctagatc caggtgaagg
    46021 ttgtctgcca tcatgtatga atagatcaat acatctgcca gttcttcttt gattcgttct
    46081 aattgtgttt cttttacttc ttccggcgtt ttccattgaa aaagttctaa caattcagat
    46141 gcttccaaag aaatagagat agccaaatct ttttcgttat gaaattgtcg ccaattacgt
    46201 tcatctctaa actggttgac tttttccata ctattcattt gcttgccttc ttccaatcac
    46261 ttcttacttg attaatcata ccattttttt acataaaaaa tagatgatgc ttttaaaaaa
    46321 actcctgttt tcttttattt aaaataaatg tcatagaaaa tcaataattt aggtagccac
    46381 catgtgtatg cagtagcgtg tcttcttccc ttgaggcttt ttgtttttcg atacgttctt
    46441 cttcccaaac agacaacgct actagaatct ctcttaatga ttttccttct tcggtgagcg
    46501 agtactctac tttaggcggg acttggttat aggcttttcg atggatactt tcatcttgtt
    46561 ctaactctct taactgctgg gtcagcattt tttttgtgat cgtagggatt tgcttgcttc
    46621 tccacgccta aaataagcga tattgctctt ccctttatct ggtgtaagca aagcagttag
    46681 cattttgacg atcgtgggtt ccgcttttca cttcttatct acttagtatc atgaagtata
    46741 ctttcgtact aatactaagt agcggaagtg tcaactactt tgtttaacta catagcaaaa
    46801 gaaacagaag ggtgacaacg gtcatcacgc ttctgtttct tttgaatatg ctgtgttgtt
    46861 gtctctatct ccgtgtagac tttcctcaaa cttatccttt gacagtaagt cagtaggctg
    46921 atttgctctt tctagatgcc agatacattt ctacaaaaaa atagtgattc tgttttgaaa
    46981 attcgatttt tagttctgca ttttcgtaaa catccaacat ttttttcgca ctgtatagac
    47041 ctaacccgcc atttttttta gaggtttttc ctcttttgac catctccgat agcgtcaaat
    47101 tcgttggatt tgccttgttc gctatactaa aacgtgatcc ctctttttct tccagcagat
    47161 tgatcgtaat cggactctga tcggctacac gatgatcgaa tgcgttagaa ataaaaatag
    47221 ataaacagcg aataaaaaca accagatcaa tcgggattgt tttcacttcg acaggaataa
    47281 ctacttggat cgggattttc tccgattctg ctttttctag aaattttaaa aagacacccc
    47341 tgacagctgg ggatacaata tttgttagct gttgtatttg cggtttttca aaaatagctg
    47401 cagaatgatc ttctaatatt tctaaaaatt ccaacgtttc ttttttattt tcctgaagag
    47461 taatttttaa tgaaaacagt agacttttat aatcatgttt gaattcctct agatgatggt
    47521 aatattcttc atttatttga tacagttgaa gcgattcctg atgttgtctt tcttttactt
    47581 ggaaactgat caacataatg gaaaaaaaca aagaaaaaaa actgatgact atcgacaaaa
    47641 gagatagaag cagataatta gatgagcgga agtgaacact tcccaatacc aagtaaatca
    47701 cgccccagct taataaaata caaatacctc caaaatagtg ttcagttgga taagtaaaaa
    47761 gcaagttgag aaatctcgtc tgtttgtcta tactttttac taatagatag aaaataaatg
    47821 cgataagtag tgcaaaaaaa cttgctgttc caagatgtct ctttcctaaa taatccatca
    47881 gatcaaaaac cagaaaaaag ctgagtccag tgatagcaaa aaaaagatta ctgatcaaac
    47941 agctgataaa caggtttttc gtagtaaaat aaaaaagaaa catcaataat aagccaacta
    48001 ggtaaggaaa gctcacttta gggataggaa gtaaaagtgt caccaccatc aaagctatac
    48061 aaccaaagct gttttttact agcagttgtt tcgaaaagaa taaatagata cctaaatcta
    48121 aaatcaaagt aaaggtcaaa taaagtgtca ctgttgcgac catcttattc ctccattgtt
    48181 ttgtttcaaa atgtacacat atttttttaa taggagcctg catatgaaaa agtattttat
    48241 catagaagat aaccagtttc acagcgaatg gatcatcaga gaattggtca atcatcatat
    48301 cgagaatatc gtgcagctca cttctatctc tcaaatcaat cagttgatcc agttgggcac
    48361 tattgataat cacgatgttt tctttatcga tatcgacttg aagcaatcac tcacaggtat
    48421 tgatgtcgcc gaacaaatac gtaaaaaaaa tgcacatagt aatattgttt ttttcaccgc
    48481 ttatacgaat tttgcagtag agatcatcaa tcggaaaatc cttccgattg gatatatcag
    48541 aaaagacagg aatttatctg aacaaattcg tcaagtgcta gaacagatca attggaaaga
    48601 acagcagatc gtaaatgtcg ataaaatctt tattgatcaa aaaggtggaa gtctcgtcct
    48661 gtatcctcgg gaaattttat atattgaaag tatcaaaggt ttgaaaaacc aagttatggt
    48721 caaaacaact acagaagata aattagtgaa tacttcttta aaacaattga agacagagtt
    48781 agaaaagtat gcttactttt tacttttaaa atcttactgt atcaacttat cgcaattgca
    48841 gaaaattgat tctgctcatt atgtgttaga atttttcaac ggtgactcct tactcgttgg
    48901 tcgaaagatc tttgaaaaga caaaggaacg tttccatgac ttccaaaaaa ctgcttcgag
    48961 ttgatgaata agagctgaga cgtaagcata gcagatttca gggagtgggc cttgaatcgg
    49021 ctccactccc tgaaatcgtt gatatgattt tacgcttcct cttctgtagg agcatctgtt
    49081 aacgaccatg tgaatgttga aacgtagttg tctacagcat aattggatac aggaacaaat
    49141 aattccactg attgtctccc ttgttccaca ttctctccta atgaataaat ccatgtgcca
    49201 atcccttcat ttgctcctgc agttactaga tctactgcct gatctgctgg gatttcgatt
    49261 ccttcagcgc cagttccaga aacggtagat ggcattccac tgctattagt tgcgctattt
    49321 cgaatcacgg cgtttttgaa tttcaccttt gaaccagtta gtttattcgt atcagatgtt
    49381 gtaccaataa attctgttcg tttgactgat aatttccaac cggcataatt tccgctttta
    49441 tccgttactt gtacgaaatt ctgtacttgt gtttgtgctc cagtactgtc cgttccgctt
    49501 tgtaatgcag catatactgt ttttgctgta cctaacttga ttgttccaaa atcaagatct
    49561 ggagcttggt caatcgtcag taatccggat gttccctgat ttggttcgtc aggtgtaaaa
    49621 ggagtatcag gatcatttgg atcaacagga ggaactggaa caacgtcttc aggatttgca
    49681 gcatctggtt tggtcacaga aatcgttcct tctgtagtgg ctgagctctt attctcagca
    49741 aaaacacttt gtgtacataa taatagactt ccagctagaa cggtacttaa tgcaattgtt
    49801 tttttcattt ctttttcctt ctttctttta aaataaataa tccagcaaat aagccaataa
    49861 gaatgactcc aacaagtatc agccatcgcc agtcaaatgt ttctggctga ttttttaaat
    49921 caatatcctt ttcattcaat tggtccgctg catcatcagt tatttcaaaa tcggtatgga
    49981 gattccagcg tttttctttc caagcaccct tcacaaccgc ttgataatgt cccggagcta
    50041 atttttttcc ttctaatggg acaagtaaat caaaatttgt attgggtgct acatctattt
    50101 tttctttttt tattgaaagg taaggctctt ttgtttcttt tttgtagatt tcaaccgatg
    50161 tagtcaagcc gaacatataa gaagcatttg gattttgaag attcgcctta atcacatttc
    50221 ttccatcgct ttgatccgca taagcttttt ttaatttcaa atctggggaa gactcttttt
    50281 cgctatttca aatcaagatt gctttcactt gagaaaactg attgacgaca ccaactgtac
    50341 ttttgttaga aacgttctct tttttttctt gaaacgtgat ccctcctgcg accacaccag
    50401 ccagctcttt ttctggcgct ttcagtgaaa gtgtctcagt gaccgcactt tgaggaggaa
    50461 cagttatttc tcccactgtt tttgctaagt ttttcatatc ataacttaat gtttcatcaa
    50521 atttactttt tgattctcca tattcgatca caccgtttga attcgtcgta gcggagtgga
    50581 tcaatgtctc aactgttact gatttatctg ttcggttaaa gaccgtgtag gggatttcct
    50641 gtgttttccc aggctccatc cttacattta tatatgtttt gttcttatca atctgattag
    50701 ccggtaattc aaaatctacc gcaaaattga attcggaggc atggacaggt gtctgccaaa
    50761 ggatgaatcc aaggagaagg atcataagta aagagcagtt gtttcgatga cttttattcc
    50821 atattcgatt catcaatcat cctccttgat ctttattctg tcggcgcatc tgctaatgtc
    50881 catgttatct ctgctgagta attaccagct atctgatttt ctttggttaa gacaagttta
    50941 aacgcccgac cgttatttaa atattctgaa taatctgctt gcacattcgt tgaattttgt
    51001 cctctgacaa tttccagttc ttcttcattc actaattgat actcctctcc tttcttgaaa
    51061 tataggacat cctgcataga aaatcctgca tcatttgtga aaggctgaga taattttatc
    51121 gttaagatcc acggattttt cgtttctcgc tgatcataaa tccataattt ttcctgtata
    51181 tccatttggc caaatgcttc ttgtgtcaaa ccagttgctt tgatctgacc aaaatcgagt
    51241 ttggaagggg caagaaatga gagaccacct tctactacga tcttcgtagc aggtgcaaaa
    51301 ggcggggcat catgataaac aactggcgta tcacttggat tgtgattccc aatttcatcg
    51361 ttggtacttg gcttgttctg cacaccctta gcagtagctt ctcctgcttg gtcgtttaat
    51421 acaatttcta atacatctcc tgcaaaaagt tgattttctg ggatagaaag aacaaattcc
    51481 cctgtatcat caatagttgt ttctgacaaa acagcttgat tttttttcaa tgaaaccaag
    51541 gctcctgctt cacctgtacc tgtgatcgtg cgagaagctg cattgatcaa tgtggtactt
    51601 aaattagcag gttcaggagg aatcacatcg actgtcgttt tggcagagtc cagttctgtc
    51661 acttcttggc cacctcttac ttttttagca gcaacaactt gcacttgatc ctccgttttt
    51721 aatctctcat cattgggtac ttgaacttcg aacaagccgc cttgttcgcc ttctccccat
    51781 gggttatatc ctgccccttc attttcatct gtttgtcctt gcgtgagagc agtaatttct
    51841 acagatgatt tcgtcgtatc tgcgtaagta atacgtaaag tcacttctac ttcgcctgtc
    51901 caagcatctc ttggctttgc ctcgacacct tcgggaacaa cgacatgccc atagattttt
    51961 ttatctgcat tcgttggcac tcttaaggat tcaatcatcg ggagctgatt attggcactg
    52021 attcgattat aatttttcat tccggcacct gtctgagcaa attcatttgt taaacctgca
    52081 ctagggcttg atacgaaatt gcctaagtcg gtcccattga tcgataagtc tgtcatatca
    52141 gaaaaataat ccgggtcttt ttcaaagtta tcgaatgttg cgcctgtttt tcgccataaa
    52201 gcgatttctg aatgggcgat ctttaaagaa gaatctccat ttccttggaa tacccagccg
    52261 cctatgccat tggtctcgct tccaatccgt ttgttttgga aatcaaaata ggtaggagat
    52321 tctaagtcca cattcaacac atccccactg atgatgccgg tcttatcttc ccctgttttc
    52381 ccgaccatga ctaaataagt tttttcacct gccgtaaaat ttaaatcaat atctccagtt
    52441 gtatctacag ttgctccgga atctgcgcgt atatcgatat ttgatttttc tccagtgact
    52501 gtaaaatcag ctgaacctgg actttctaga aaaccagaag cgtcataaaa ttggatcgct
    52561 tgatttcctt gattgagtct gttcacgtaa gatttgccat cacctgtatt tcttaccctc
    52621 aatgagcctc cattggttac tgtcagcttt tgcgtaccat tctcaaaacg aactgcgctg
    52681 gaacgcccac tctgcttatc gatgtcgata actgatccgg ctccttctac gatcaaagaa
    52741 aggccttttc ctacaaaacg catcccattg ttcccgggtt gatcgtcatc tccagccatt
    52801 ttgaccgaga tttgtccacc acttgataca tggatttgac tagaatctcc ttgcaagcga
    52861 aatccgcccg tagttgctgt attgatggtg attttcccac cattcgatac agtgactttc
    52921 gatcctgaac ctctcatttg gatgattccg cgattgttgt cattggtaat attgatcttt
    52981 gtatttaccg aaaattcgct tcccgaacca ctgacttcaa acatgacatt accaggtagc
    53041 gtatcgactg cctctccttc atcgctaacg ccatctgccc gatcataatc aagagagacc
    53101 tttgaaccgc ttaagatatg gattcctggc gctgtttttc cactaccagt ctcatctcct
    53161 gaatctagaa aaccgtagaa tttccctaga tacggtccat aaagttgact accattgatc
    53221 acattcaatt gatagcttct acaaaaatag gctttacccg gagcggcatt cagtccttcc
    53281 ttcactggtt ggacatccaa cacaccggaa acaccatcta atgtgacagt tgcacgagga
    53341 gcgtaaatca gtcctaattt ggacccatca tacaaatcaa gtgtcccggt aaattttatt
    53401 tctcccgtaa aatgatatct gcttccccaa aaatcggtca tataagcaat gataatggca
    53461 tttccatcga tatttggtgt caatccacct ggatgtgcag ctgttccttt aaaattctgg
    53521 aactcgatcg actgaggtat ctcattgata taaatatggt gtgtgccgaa attgtgagcg
    53581 aatccaccaa aatcaataac gatatctcgt ttgatctcta attccctgtc taaagtcaaa
    53641 ggctgaatca gtcggatctt agttactgat tcgtctgcga gaagctgcgc cagttcttct
    53701 gttgtggcag cttcgctgta cccatccgct gaaacagctc ttgccagtgc cgcttcactg
    53761 ctatcagacg tttgttcagc tacttctgag gtctcacttg tcttattttc ttcttgcctt
    53821 ttattagtag aagcctcact ttgagattct gtagtttcag aagtaatcga ttcttctgaa
    53881 aaagtagtct tgtcaaaact ttgattctct gattgcgttg attcgtttgt tttctctaca
    53941 tcggcaaaaa cggatgtcac catcaagaga gggaagacaa acaaaatcga cgcaatcagc
    54001 ttttttacac attgattcat tctccaccat cctctctgac gttttgacaa ttttatttta
    54061 tcgttttaat ctatttccaa ttgtactttc atatttaaaa tatgtatttt tatggaaaaa
    54121 tttttctatt aggtgtaaat ttcttttttg tacaaaaaaa aggctgtgac aaaagtcttg
    54181 tcacagtccc ttatcccgaa caagcggtgt tctcccgcca actcctcggt cacaacctcc
    54241 attttaacaa taaacctgta aatgtgaggg caatactttg attgtcacag gtaatttatc
    54301 cccttcatct ccatcaacat tcgtcgtcaa ttcttcctca tcagctaatg acacaactaa
    54361 ttgctcacaa gtaaggtagg ctagattttt cgtagattca tctactcctt ttaataggtc
    54421 tggcaatgtt ttaagcgtat cgatcaaaga agaatctttt aaataaacca aatggagctt
    54481 accatcatcc acttttgcat ttggaagtaa tgtttcaaaa ccgccgactg agttagttag
    54541 tcctatcagc aaggtactgc tctcaatttc ttccttcttt ccatcaactt ctacgtgaaa
    54601 cgagtaggat tgtgtggaag ctaactgttt gattcctgac atgaaataag ctagttttcc
    54661 aaatttcgtc ttcttctccg catctacatc attgattgat tcagggatag agccaattgc
    54721 gacgacattc ataaaatagt cgtcatttat ttttccaata tccaatggct tgactgattc
    54781 aatcgaaaaa tgattgatcg cttcctcagg ttctagcggt atccctaatg ctcgagccaa
    54841 gtcattcacc gtacccaatg ggaaaaatcc gaaatttggc cgatgttctt gttcagcgat
    54901 tccactgatt ccttcgttga ctgttccatc tccgcccatg acaaacacac tatgataccc
    54961 ttccgtagcc gcttcgcgag taaagttttt tgcatcccct gctttttttg tatgcaagac
    55021 gaccacttca tcaaacaccg attccaattt tgctatcgct aattgctcaa attcctttgc
    55081 ttgttcaccg cctgaactgg ggtttaccac taataaaact tttttcatca ctttctttcc
    55141 cttctgattg atttctttaa tataacgata gcataaagaa aaaaattagg ctgtgatgaa
    55201 gtactgttca tgaaacattc atttattttt tgacaagata aaacatccat actatgaaac
    55261 tgaggaaaat taaaacaatg atataggaaa taaaagaaaa tgataggcat tgtataccta
    55321 ataaagtaaa aatcttaggt ggcagataaa aacacaaaaa tagcaagaga atgtaatagc
    55381 gtttcttcca ctttttgtta tcagacctta tagcgatcag aggaatacca aatgcataac
    55441 tgaaaaactc ttgatgtact gttaatatgt cacggccttg aataataaat gcgacaatcc
    55501 ccgctataat tagtgtgcct aacaccaaaa cggaaaattg atgaatatat tttatataca
    55561 aatgatccat tgtacttcct gcctttttat ttgtgagaaa aacaaatccg tatacgtgaa
    55621 taacttctag aaataaaaga aatttgcaaa accactccat ttatctactt ctacgtgcat
    55681 tttttgatat tgttttcttt atttaagatt atacagaaaa atatctaggg tcgctggatt
    55741 ctaagtaaaa aaaattttta aaataaaaag aactaaatca agtagtaatt aaactactgt
    55801 tgatttagtt ctaataaaaa gatttagatg aacagtttta ttgatttaaa tagttcatat
    55861 tttttattta taccgcttat ttactacttg ctgtactaga atctgaagtg aatgtactca
    55921 aaacattttc aaagtctttg tctttgattt ttacattagc gtctttcaat acttcgccaa
    55981 ttactttatt ctggaaggta ctgtcactta attttgtgtc tgttgcaatt tctttcaatt
    56041 cttttttgta tttatccatg tcgttgcctt tgttttggtt tttcaccatt ttaaccacat
    56101 aatattccgt agtataagtt gaagcattgg tagaagtgat cacatcagaa acttgaccat
    56161 ctttcaactt aaaggctgct tctttgactt ctgccggaac cgttgtagta gtggaatcaa
    56221 atttcacttt tccgccatct tcttttgtcg tagtatcagt tgacttgtct ttggctaatt
    56281 tagagaaatc gtctccctta tcagcagctt tcttcacatc ttttgcatca tcttcacttg
    56341 ctacttggat aatttgagct tccacttctg gatggaaaga ttcccaagcc gtttttaaat
    56401 cttcatcggt caatttgata tgtttcttta gaccttcttg aaaggctaga ctttgtttga
    56461 tactgtcttt gaatgtttgt tctgtgtaac ctgcagattt caattgtgaa tcaaatgaat
    56521 cacccaattg tttctttgtt tgatcatatt gagaatcgat ttctttggtt gatacgtcat
    56581 ctccatattt atcttcaaat acttgataaa cgatcaactg ttgcacagcg gaatctgctc
    56641 cttgcatttt tttcaagcta tccattactt cttgttcgct gattgtaccg cctttataac
    56701 taacggctgt tttttcaccg gcattcgtac agcctgacaa taagccagtg cctagtacga
    56761 tggcaagtgc taaaaatctt tttttcattt atcaattctc ctacttttcg ttttttgcga
    56821 attgctaaaa acaaaggtgt tcaagatgaa aatcatcaag aacaccttta ctaatctgct
    56881 cgatacgtcc tgaatagata taaggctaca caatccagaa agttcgctca gcagaataaa
    56941 tgtcaagtag cacatcacac cttaatatac tacttctttt gttaccgttt ttcaagtcta
    57001 tcttgcgttc cgtatttaac gtaactaatt cttagaacaa atgttatcga ttttgttttt
    57061 cttcttccgc tgctgggata ttcaaatcag acacttttat gttcgatttt ccagacattt
    57121 ttttgttgtt tttattatga tatattttta tacaactaaa aatgaatata ttaaaactac
    57181 caaaaataac ttttaacgct gtttcattat atgttttttc tattttatat aaacttttgg
    57241 atacttaata ccaaccggtt ggattgtacc aacatgagaa tgtgtcagtt ctatttcctg
    57301 tcgcacttgc ataggcccat actccttttg ttgcatttgc tgatcgaata gagtttccaa
    57361 ctttcgcttt tgcaccgtga cgattataga cacgactata gtggttagaa tgacctgttt
    57421 ttctcgtaca agttgtatca ctataataaa cccatcttcc agagctatga ttaattgctg
    57481 ttctagaaat ttcccggtct actggtaaat ttgacttaat aatattacct ttagcatcaa
    57541 aatgaatctc tcctggcttt aaagatttgt aatagatttc accgtgaact gaatttgctt
    57601 ccacttcaga tgacatagcg tttcctccaa caaaacctat ccctagtgca caaataataa
    57661 ctgctttttt cataaaacga catcctttcc tttttgattt ttaaagcatg atcttcttgt
    57721 ttcctatttt ggtcagatat ggatcatgcg tgactacgat aactaacgta ccttgggaag
    57781 cttgttgcga taagatttca actactttat ccttatttct tttatccaat gacccagtcg
    57841 gttcatcggc taaaatgatt ttgctattct tcaataagac tctcgcacag gcgattcttt
    57901 gcttctcacc accagataat gtgtagatag gcgtacttag cttcttgttt atatttactt
    57961 ttttcaatgc ctcatacttt gctgttcgct tatctgaaac atttaaattc gaaaacttca
    58021 atccaatatc taaattaaaa tcgattgttt catattcaat caacccaaaa tcttgaaaaa
    58081 gaaaagatat ggtatttctt tttatcaagc gcttctctct cccagttaat tgtttcgtct
    58141 cttgatcgaa caaatagaat tgtcctgaac tgaactgatc attcaatcct aatatattca
    58201 ataacgtact tttacctgct ccactttctc caacaacagt cacaagatcc ccagcatccg
    58261 cagtgaaacg atccaaatga agaatcgttt tgtttcgaat gatttttttc gcacctttta
    58321 tcactatcaa cggctccaat tgttaacctc cattcatcag caataactga cttttctttt
    58381 ctgtagacaa aataaaaaca actgaaagaa taaaatcaat gaagctaaag aaaaaaatat
    58441 taaaaagata tttaggctct ctttctataa gaaaaaggaa gattacactt gtcgttcccc
    58501 atttgatgag gttcatcatg aagaaatctt tatacgtttc aaagagcgaa taaccacttg
    58561 aacgaaagat agcgattttc cgacaattgt agacaaaata tatgcataac atgacatagc
    58621 tcaacaagaa gaaagaaaag aaagtgagta aaaatatctg tatcaagtaa tgaaatgatc
    58681 tgcttaacac tttgacagct atctcttcag tctgagtata cggaaatgat aactgcaagt
    58741 tatcacgtaa ctgactttct tcaatcagct caaataacct ttctttcgac aaagagccta
    58801 tcttgatctt taacggtgga tagatttctc cgctgagaat atttctttcc aaatacatac
    58861 tgtttttcaa tgtcagtatt tcaacaaccg gataatgttc gatccagggg atagaaggaa
    58921 taaatgtata aatgggttga ttgtttttta taaatagaaa tttcggttct ttttcactga
    58981 tttcttgata atattctgtt atttgcgcca ggtcattttt taaatctgta tgtctatatc
    59041 gttcagggat caatataaca gggtgcgttt ctttttcggt tatttggatc aaccttccag
    59101 agacatcaat caaaggaaat ttcttaagat aattcagatt tatttttatc cctctgccaa
    59161 actgttcatt ttcttcattc aagtaatctg atacattgac taatatactt ccgtttttat
    59221 tcaaagtttc atataattcc tgatctgctg ctgatgcaaa acgattggaa taaataaaat
    59281 ccacgtggtt tttcccgatg gttactggat aaaatacatg atagtcctca ccagcgattt
    59341 cttttggtgg ttcatgggaa acagctaact gtgaagaaga aaaaacgata gttgctaatt
    59401 cgacattggc aacggtcaaa agaagagaaa acaacaatcc aaatgtaaaa agaatgtatt
    59461 ggatatagtt gaacgcttct cttaacgaag cgattttttc aactaataaa agagaaatca
    59521 aagagatacc atatattccg ctgaacaaga aaacgtacat ctggaacaaa taaagaaatt
    59581 gctccttata atacataaaa ataaaaccac caatgattcc tgcaattatg aatgtaataa
    59641 tgattttctt tttccccaat aaacaccaca atacttgatg taatgaaaag tcattattca
    59701 acaataaact gatttcttta ttcatcaata agaaaaagga tgataacaaa aggaataaaa
    59761 gaatcaatcc aatagttaaa tacactcgat aattatatac gtctgtattt gaaataaccg
    59821 aatacgaata gctattttca aagtcttcaa aaatattagt ggcataatca gtgtgaaatg
    59881 cttgattgaa tcgatcagtg aatagtgaaa tgaaccgttc ataggcttct ttttctctcg
    59941 tcatcaagaa aaattcacct tcaaaatctt ttattttgat cgattcttca atggacgtta
    60001 cgcgaaagga ggtattagaa tagggtacct tctcgtgaga agatttgaca tccccttgat
    60061 aatacaatgt catttcaagc tttggctgta tcttgaagtt ccaagatgta tctacttcat
    60121 tggattcatt tactgttttt ttcatataag aaagctcagt ttctgatgag acattttcaa
    60181 ataccatgat tagctgctca tattcttctt gcccaataaa atgttgtggc aatcgaatcg
    60241 gagtataagt atctgtttga tggaaaaatt catttgtccg atagagtacc tgtttattta
    60301 tactaaccaa ggcaaaacca ctcatgatac cggtcactat gattgtgagt aaaactgtga
    60361 actttttttt gattatcacc actctccctt acttaaatga ttctttgata aaatcatacc
    60421 gtttttcatc aaaaaaacgt atctatcaag tgtataggtc atgtagaata aatctatata
    60481 ctgtagaatc gaaaggagaa gaagatgaac accggagaaa aaatacgaac ttaccgcaga
    60541 aattgtaagc ttacgcaaaa agaattaggc gaaaaaattg gtgtttccga taaaacgatt
    60601 tctagttggg aaaattctcg tacaatgcca gaccttgaaa tgctgtcact tttacatcat
    60661 actttaggtc tgccgattga ttttccttct attgaatcag ctaccgaatc aatagaacca
    60721 acattgccag atgaacctct tgttaacaag aaatcttatt catcaaaaca acccttctca
    60781 ctcaaaaaga cactatcttt ctttctgctc gtcttttgtt tggtctatct ttttcttatc
    60841 aattatcaat acaaaaatga aacgatcgat cgattcaatc cttttttgaa accacaaatc
    60901 ggctatacca cactccctgt aggatacgtg gacaattcca tcgaacgttg ggtatgtaat
    60961 gatatctttg gccatggtac atggctgtct tttcacgctg gagaggtaga aaaagagaaa
    61021 aaccatgctg taatcaaaca caaaggaaaa cacgtcatga acgttcgcct gctcacttgg
    61081 gaagccattc cctcagatta ccaagaaatc ctcagccaag actatgacga cgccttaaaa
    61141 agaagccctc aggagccaga taggcattaa gtaggagatg aacgtagaga tagaaaaaat
    61201 caatgcacat gatcttatcc aaaaaacagg ggtacaaatt aattgaaaaa gccttgagat
    61261 gaaagttcct aaataaaaaa gttcagaatg ataggtattt ctggcttata gtataatatt
    61321 tacgccacag cctcccaatg attctttttg gaatcataat caaggatgga cattcacaaa
    61381 aatattttgc tcattcgatc cgaatagatt aacgaccaat ctagtaagta atatagattg
    61441 gtacgtagtc aagctgcaca taatttagat caattttagt tcttttttat atttctatga
    61501 tctgctcatt tacttttata tgatggctta cagtaacata aataaatatg ataatcgctg
    61561 gtagtttatc caagagtact caataaaata caaaatggat tgttcaagcg cctatcataa
    61621 tgataaaatg aaacaaataa acgatacaag gaggaagaga ctttgagtaa tggcagaaca
    61681 attttttcat tgtaaaaatt gttcagttgt taatctatgg gatttcggta gcatgggcat
    61741 ttttttatag aggaatcaca ccaacctggc cgttttatct cttgttagtg ggaatattag
    61801 taggaaattt tgcccctctt cactatcgta cgaacttttt tccaactgat tctggccttt
    61861 ttcttaaaac aaagacgcga aaattagaaa acattattga aatgacaatt acctgtataa
    61921 tcattttctt agttatattc ctaaacgtct tctaataaga atctcttaaa attagcttgt
    61981 tacagttagt tacctagttt atataacaca gttatcttat cttccttatc aacatctttt
    62041 gccaactctt tgtcttaaat tcgaaacata taagcagagc tacgaacacc aagactgttt
    62101 cggctctgtt ttttgcatca aattcactct tatatagctg ataatctcgg ctctttaaaa
    62161 gaaattattt tcgcttcatt ttattataaa aaggctagga accttgatgt tatcaggttc
    62221 ctagccttta tttaagtatt accccacaga cccttccata tagattttta aattacacac
    62281 tagtttaaac taaaacaaac aaggaaaaac ttaccattta atcaaaaaat tagctcatat
    62341 gaatatatta gaatgtaaat tagactggtc aaaatctggt caaaaaagag gacgaataca
    62401 ggtagtcctc ttttttgtta ttttgatatt ctaaatcatt ttttattgga ttattccctt
    62461 gttcgatata gacccaaatt gatctatcaa taattatcaa actgagtcat atagagacaa
    62521 aaatttcaaa aatgacctgt taaatggtca tcgaatatca tttaaaatca atacgacgga
    62581 gtcagacacg gagtcaaaac aagcccgctg agagtaaaaa atatacagtc ccataggtaa
    62641 agtgccacag gactgtatat ttttttattt gagattacct gaaatgattc catttaattt
    62701 ttatctcatt ttttcctact agttagtatc aaatactctc ctattttctc aataaactat
    62761 tttgggttac atggtatctg aaacagctaa aaccttgtca ttatagcaaa aaatcacttt
    62821 atctccgttt catttcagat acatttcact taagcggatt ccaaaaaaac aaaagaaaaa
    62881 ggaaccccta gttagcctat gctttttatt ggttcctctc aaaacacggt catataacaa
    62941 cggggtg
//
LOCUS       DARBET010000007        60875 bp    DNA     linear   BCT 07-NOV-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4027
            SAMEA114569841-rid20112183.denovo.007, whole genome shotgun
            sequence.
ACCESSION   DARBET010000007 DARBET010000000
VERSION     DARBET010000007.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA114569841
            Sequence Read Archive: ERR12203666
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 60875)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 60875)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001972618.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001972618.1
            Genome Coverage       :: 132x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/07/2023 13:18:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,041
            CDSs (total)                      :: 2,975
            Genes (coding)                    :: 2,908
            CDSs (with protein)               :: 2,908
            Genes (RNA)                       :: 66
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 67
            CDSs (without protein)            :: 67
            Pseudo Genes (ambiguous residues) :: 0 of 67
            Pseudo Genes (frameshifted)       :: 26 of 67
            Pseudo Genes (incomplete)         :: 28 of 67
            Pseudo Genes (internal stop)      :: 20 of 67
            Pseudo Genes (multiple problems)  :: 6 of 67
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..60875
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA114569841-rid20112183.denovo.007"
                     /isolate="VRE4027"
                     /db_xref="taxon:1352"
                     /country="Denmark"
     gene            199..2733
                     /gene="secA"
                     /locus_tag="SCJ86_000544"
     CDS             199..2733
                     /gene="secA"
                     /locus_tag="SCJ86_000544"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631358.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecA"
                     /protein_id="HEG1168130.1"
                     /translation="MANLIRSLIENDKKELKRLDSIAKKVEAYADQMAALTDEQLQAK
                     TAEFKGRYQKGETLDQLLPEAFAVVREAAKRVLGLYPHHVQLMGGIVLHDGNIPEMRT
                     GEGKTLTATMPVYLNALTGEGVHVVTVNEYLATRDSTEMGELYNFLGLTVGLNINSKS
                     SDEKREAYNCDITYSTNNELGFDYLRDNMVVYRHQMVQRPLNYAIVDEVDSILIDEAR
                     TPLIISGQAEKSTALYTRADNFVKRLKEEEDYKIDVQSKTISLTEAGIEKAEETFGLD
                     NLYDIENTALTHHLDQALRANYIMLLDIDYVVQDGKVLIVDQFTGRIMDGRRYSDGLH
                     QAIEAKEGVEIEDETKTMATITFQNYFRMYKKLAGMTGTAKTEEEEFREIYNIQVYQI
                     PTNRPVIRDDRPDLLYPTLQSKFHAVVQDIKDRYRKGQPVLVGTVAVETSELLSDMLN
                     QERIPHEVLNAKNHFKEAEIIMNAGQKGAVTIATNMAGRGTDIKLGLGVRELGGLAVI
                     GTERHESRRIDNQLRGRAGRQGDPGMSQFYLSLEDDLMKRFGSERIKSFLDRMKIGEE
                     DAVIQSKMLTKQVESAQKRVEGNNYDTRKNVLQYDDVMREQREVIYKQRQEVIMEEKS
                     LSKPLMNMVKRTISRVVDAHTQLEKENWNLESIIDFAGSTLVHEDSITLADIEGKTAQ
                     EIKEYLNERAQEVFNTKAAQLNGPEQLLEFEKVVILRVVDSKWTDHIDAMDQLRQSIG
                     LRAYGQNNPLVEYQTEGYKMFEDMVGAIEYEVTRIFMKAEIRQNVQREQVSQGTASHS
                     EDGDAKENSNTSAKKQPIHVNKVGRNDPCPCGSGKKYKNCHGKNAN"
     gene            2859..3960
                     /gene="prfB"
                     /locus_tag="SCJ86_000545"
     CDS             join(2859..2930,2932..3960)
                     /gene="prfB"
                     /locus_tag="SCJ86_000545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_139124933.1"
                     /ribosomal_slippage
                     /note="programmed frameshift; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor 2"
                     /protein_id="HEG1168131.1"
                     /translation="MEISEIRNLLTEMQEKVTSFRRSLDLDQLEEDIAEAEAKMAEPG
                     FWDDSEAAQKVISENNANKETYDQFHSLAEELEEIELMVEMLQEEPDAAMQEEAETRI
                     NALDEKMKTYELSMLLDGPYDRNNAIVELHPGAGGTESQDWGSMLLRMYTRWAEQHGY
                     QVETLDYQAGDEAGIKSVTLLIKGHNAFGYLKSEKGVHRLVRISPFDSAKRRHTSFCS
                     VDVMPELDETIDIEINPDDLKIDTYRASGAGGQHINKTESAVRITHLPTGTVVASQAQ
                     RSQLKNREQAMGMLKAKLYQLEVEKKEQEAAALRGEQKEIGWGSQIRSYVFHPYSMVK
                     DHRTNVETGNVQAVMDGDLDLFIDGYLKMHLQ"
     gene            4065..4751
                     /gene="ftsE"
                     /locus_tag="SCJ86_000546"
     CDS             4065..4751
                     /gene="ftsE"
                     /locus_tag="SCJ86_000546"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005237293.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division ATP-binding protein FtsE"
                     /protein_id="HEG1168132.1"
                     /translation="MIEMKDVMKKYSNGTTAIRNLSIVIEQGEFVYVVGPSGAGKSTF
                     IKLMYREEKASKGTLNVAGHDLIAIKNREVPYLRREIGVVFQDYKLLPRKTVYENVAY
                     AMQVIGRKPRDIKKRVMEVLDLVGLKHKVRVFPSELSGGEQQRVSIARAIVNTPKVLI
                     ADEPTGNLDPENSWEIMKLLDRINAQGTTVVMATHNSTIVNTIRHRVIAIENGRIIRD
                     QAEGEYGYDD"
     gene            4735..5628
                     /locus_tag="SCJ86_000547"
     CDS             4735..5628
                     /locus_tag="SCJ86_000547"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEG1168133.1"
                     /translation="MDTMIRTFFRHLLESIKSLKRNGWMTVASVSAVTITLTLVGVFM
                     AVIMNATKLAQDIEGNVDVSVFVDIGTKDEEMKKLEKELNDLDHVKKVEFSSKEQELK
                     KIQNEMGDSWKLFEGDNNPLYDVYVVSATDPSYTKQVAEEAADLKNVSRSDYGGASSD
                     RIFQIAGAVRTWGLVAAALLLFVAMFLISNTIRITILSRQREIQIMRLVGAKNGYIRW
                     PFFLEGGWIGLIGAILPILIITFGYAWFFQLINPSLLRSHYSLIHPQNIVWKINLLMV
                     GIGVIIGSLGSIISMRRFLKF"
     gene            5780..7228
                     /gene="cls"
                     /locus_tag="SCJ86_000548"
     CDS             5780..7228
                     /gene="cls"
                     /locus_tag="SCJ86_000548"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010782552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cardiolipin synthase"
                     /protein_id="HEG1168134.1"
                     /translation="MKIIVDNFFTILLIMNILLSFIIVFRERKETAQTWAWVLVLMFI
                     PVVGFILYIFLGRGISKEKIFDLKIQGKIGKNLEIEEDKQALMRGLYPHPPTGQVDVK
                     QLIYMLTVFESTLYTTKNEITLFTDGREKFDALIEDIKQAEDHIHFQYYIYRSDALGE
                     EVRDALTEAARRGVKVRVLLDAWGSTQVSSSFFQNLKKAGGEVAFFFPLFVPYINPRI
                     NYRNHRKIVVIDGKIGYTGGFNVGNEYLGLVKKFGYWRDNHLRIYGEAVYTLQNRFLM
                     DWNSQHTKEVGYSPKFFPSIHSTGDIAVQIVTSGPDTEHEQIKMTYLKMISLAKREIL
                     IQTPYYIPDGSIHEALKLALLSGVKVHIQIPNKPDHLLVYWATYSFAAELIEYGARIE
                     TYENGFIHAKTMIIDGGIVSVGSANIDVRSFRLDFEVNTLVYDERMASRVRKAFFEDS
                     KISTHLTKEMYENRGIIIKMKEGLARLISPLL"
     gene            7565..8422
                     /locus_tag="SCJ86_000549"
     CDS             7565..8422
                     /locus_tag="SCJ86_000549"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292763.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter substrate-binding
                     protein PstS"
                     /protein_id="HEG1168135.1"
                     /translation="MKKLKLFLPIIGLAILLSGCSKWIDRGESITAVGSSALQPLVET
                     VAETYQTENPGKFINVQGGGSGTGLSQVQSGAVDIGNSDLFAEEKSGIDASALVDHRV
                     AVVGITPIVNKDIGVKDISMENLKKLFTGEIKNWSEVGGKDQPVVILNRASGSGTRST
                     FEKWVLDGETAVQAQEQDSSGMVRQIVADTPGAISYVAFSYVSDDVDTLNVNGVEPTD
                     ENVTTNKWTIWAYEHMYTRDHPKKLTKEFLDYILSDEVQDNIVGELGYIPVSHMKVER
                     DWQGNIVKE"
     gene            8537..9457
                     /gene="pstC"
                     /locus_tag="SCJ86_000550"
     CDS             8537..9457
                     /gene="pstC"
                     /locus_tag="SCJ86_000550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter permease subunit PstC"
                     /protein_id="HEG1168136.1"
                     /translation="MEDVKKVLLTKSKRSKMEQRGKFISFLCIALIVLVVLSIFYFVA
                     SKGLATFFTDKINVFDFLFGTDWNPSQIGANGKPMVGALPMITGSFIVTFLSAIVATP
                     FAIGAAVFMTEISPKNGTKILQPVIELLVGIPSVVYGFIGLSVIVPFVRSIFGGTGFG
                     ILAGTFVLFVMILPTVTTMTVDALKAVPRHYREASLALGATRWQTIYKVVLRAAVPGI
                     LTAVVFGMARAFGEALAIQMVIGNAALMPTGLTTPASTLTSILTMGIGNTIMGTVENN
                     VLWSLALILLLMSLFFNIVIRMIGKKGELK"
     gene            9457..10341
                     /gene="pstA"
                     /locus_tag="SCJ86_000551"
     CDS             9457..10341
                     /gene="pstA"
                     /locus_tag="SCJ86_000551"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010764435.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter permease PstA"
                     /protein_id="HEG1168137.1"
                     /translation="MNAKRSDKIATGVLYIVSGIIVLILAALLLYILVRGIPHISWSF
                     LTEPSKAYQEGGGIGIQLFNSIYLLLITMIISFPISLGAGIYLSEYAGKNWLTDVIRT
                     SIEILSSLPSVVVGLFGFLVFVIQFQYGFSIISGALALTVFNLPLLTRTVEESLQAVH
                     YTQREAGLALGLSRWETVIKVVVPEATPGILTGVILSSGRIFGEAAALIYTAGQSAPA
                     LDFTNWNPLSVSSPISIFRQAETLAVHIWKINTEGTMPDGVSVSAGASAVLIIAVLLF
                     NFGARAIGKRLYRKMTSA"
     gene            10356..11162
                     /gene="pstB"
                     /locus_tag="SCJ86_000552"
     CDS             10356..11162
                     /gene="pstB"
                     /locus_tag="SCJ86_000552"
                     /EC_number="7.3.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002408906.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter ATP-binding protein"
                     /protein_id="HEG1168138.1"
                     /translation="MKKYNWNETNVITMPKEKEVALYTDDLHVWYGDNEAIKGVDLEF
                     EKNKITALIGPSGCGKSTYLRSLNRMNDGIANTKITGKIMYQGVDINAPQVDVYEMRK
                     RIGMVFQRPNPFSKSIYDNITFALKQHGEKNKKKLDEIVETSLKQAALWDQVKDSLDK
                     SALALSGGQQQRLCIARAIAMKPDILLLDEPASALDPISTGTVEETLVNLKEDYTIII
                     VTHNMQQAARISDYTAFFYLGKVMEYDKTNKIFTRPKIQATEDYVSGHFG"
     gene            11178..11936
                     /locus_tag="SCJ86_000553"
     CDS             11178..11936
                     /locus_tag="SCJ86_000553"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317877.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter ATP-binding protein"
                     /protein_id="HEG1168139.1"
                     /translation="MTKEIISSKDLHLYYGKKEALKGIDLSFNQGEITAMIGPSGCGK
                     STYLRSLNRMNDLIPNVTITGSVLYKDKDIYGPKTDVVELRKEIGMVFQQPNPFPFSI
                     YENVIYGLKLKGEKDKKVLDQVVEESLKAASVWDDVKDKLHKSALSLSGGQQQRVCIA
                     RVLAVNPEIILLDEPTSALDPVSTGKIESMLLELRERYTMIIVTHNMSQASRISDKTA
                     FFLDGHLIEYNDTKKIFMNPEKQETEDYISGRFG"
     gene            11949..12626
                     /gene="phoU"
                     /locus_tag="SCJ86_000554"
     CDS             11949..12626
                     /gene="phoU"
                     /locus_tag="SCJ86_000554"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010723432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate signaling complex protein PhoU"
                     /protein_id="HEG1168140.1"
                     /translation="MLRTQFEEELLNLHNQFYEMGMMVSSAVHKSVRAYIKHDKILAQ
                     EVIENDININNMETRLEKKSFEMIALQQPVTTDLRMIITVMKASSDLERMADHAVSVA
                     KSTIRVKGQTRIPEIEKEISDMSDYVKKMVDNVLVAYVKTDEKDARTIANMDQRVNDY
                     FNRIYNATIKNMQENPETVISGTDYLNVASYLERIGDYVTNICEWIVYLATGKITELN
                     TNHNEIF"
     gene            12864..13895
                     /locus_tag="SCJ86_000555"
     CDS             12864..13895
                     /locus_tag="SCJ86_000555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YibE/F family protein"
                     /protein_id="HEG1168141.1"
                     /translation="MQKKISIITIFFICVLTSWVIQHQAALYEVPVIEIEKVRIKEDH
                     QLITGKLMNTDQKGESIYLSVPYQKNQIETANIKARQMYLVQMHGDEWQLVAKKNDGW
                     LFFFTSIFIGAMLLIGGKQGLFALGSLIINIVLLLLLLLIFTKTEGISLLNISILFVV
                     IGMIISILALDGWNRSSIIKISAAVTSVFLAFFICLLTMNHWKDQGLRYEELNYLTRP
                     YRSVFLSSVLIGTAGGTLDTVITVIATLEELTKRQPEITAKQLWLSGRQVGRDVIGSM
                     TNILLFVYISGSVPSLLLYLGNQWSFKETIEQHLSLEFLRVIAGSLGIVLSIPISVLF
                     FLTVRRLKR"
     gene            13892..14659
                     /locus_tag="SCJ86_000556"
     CDS             13892..14659
                     /locus_tag="SCJ86_000556"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289493.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YibE/F family protein"
                     /protein_id="HEG1168142.1"
                     /translation="MNVLIILVGILAISVLLIGGTQGMYILLGLFINLGIFFLLLFGY
                     HQKWPILALSVIGFLLIAVVILFFINGYNLKMRAAFVSILIFLFCFLLLLPITDFLAI
                     QGFTSVELEELSGLDKTLAIDFRLLTRSLLLISLSGAVLDASVAISSGTFEVYQANPQ
                     LSFYQLRHSSFAIAKKILASTVMTLLFAFMGSSLALILWFIDLDIPFQQVINEKSFVL
                     EYTMAILTTLSALLVLPLTCVTTAYLFTKKKEQLKME"
     gene            14759..16327
                     /gene="liaX"
                     /locus_tag="SCJ86_000557"
     CDS             14759..16327
                     /gene="liaX"
                     /locus_tag="SCJ86_000557"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335990.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="daptomycin-sensing surface protein LiaX"
                     /protein_id="HEG1168143.1"
                     /translation="MKERERILDLVKKGILSTEEGLDLLESMATEKDEKQIKKEADKV
                     TASHKEKDQASQLIDKLENGEEEISEPVDPKERERQDQENLEKILDELATEANKTSAR
                     LDEVNAQFADNKAARNEKQEALMQLNTKEELGELTEEELAQRQTLEAEIKELEASGDT
                     LLEEQLKLEAELKDIRKNQWSEKKETFTDKFELPDDWKDQATDTLNQVGEKMSEAGSQ
                     LGKFLKKTFQTVSETVNDNMEWKDVSLRVPGIATTKFEHEFYYEAPAASILDIKAANG
                     NVTLKTWDSNDVKVEAKIKLYGKMGAEPFEAFSERSQIEVNEDHISFQIPNKRVRADL
                     VFYLPKRVYDHAAIKLLNGNIMIETLEAKDIYTKSTNGNIIVDQLTATMLEVEGVNGN
                     IDIRNGNILDSIIETVNGTVTFGATAENLSVSLVNGDVRLTIKEDNLKKVEASSVNGN
                     VKVALPDGIGLEGHAKTSLGSINSRLSNYEVVREKKERTNQMLQFRRVSDGEIAQVQL
                     STTTGSIYLKDTDK"
     gene            16624..16944
                     /locus_tag="SCJ86_000558"
     CDS             16624..16944
                     /locus_tag="SCJ86_000558"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629442.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PspC domain-containing protein"
                     /protein_id="HEG1168144.1"
                     /translation="MMKKKLTKSNKNVVLTGTLAGIGEYIGIDPTVIRVLYVFLSLVA
                     FGSPIILYILLALIIPSARSARTNYGHNNAYYEKNNYREQTREQTKKRKEAEKIDDDE
                     WSDF"
     gene            16944..17294
                     /locus_tag="SCJ86_000559"
     CDS             16944..17294
                     /locus_tag="SCJ86_000559"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303620.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage holin family protein"
                     /protein_id="HEG1168145.1"
                     /translation="MSYFQRILVNTLTFISLAVLFPAMVHVSSIWTAVLASFVLSVLN
                     MFVKPILTILSLPFTLVTLGLFTFVINAAILKMTSFFVGAEHFSFSSFGAALLVAILM
                     SLVNVIVSEHNSRK"
     gene            17461..18399
                     /locus_tag="SCJ86_000560"
     CDS             17461..18399
                     /locus_tag="SCJ86_000560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289568.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HPr kinase/phosphorylase"
                     /protein_id="HEG1168146.1"
                     /translation="MSEVVKVSQLVKKLSLEIVTGDEKSLDRVIVTGDISRPGLELTG
                     YFNYYSHNRLQLFGSKEISFAERMIPEERLIIMRRLCSEDMPAFIISRGLPAPEEMIQ
                     AANENGIAVLRSPISTSRLLGELSSYLDGRLAPRTSVHGVLVDVYGLGVLIQGDSGIG
                     KSETALELIKRGHRLIADDRVDVYQQDELTVIGEPPKILEHLIEIRGIGIIDVMNLFG
                     ASAVRGSMQVQLAVYLEAWAKDKKYDRLGSEDTTVEIAEVGIPQIKIPVKTGRNVAII
                     IEVAAMNFRARTMGFDATKTFEERLSRLIEENSGND"
     gene            18414..19244
                     /locus_tag="SCJ86_000561"
     CDS             18414..19244
                     /locus_tag="SCJ86_000561"
                     /EC_number="2.4.99.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002308605.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prolipoprotein diacylglyceryl transferase"
                     /protein_id="HEG1168147.1"
                     /translation="MLAQINRVAFELVGIPIYWYALIIVSGIIIAMWLSSREAVRVGL
                     KADDVTDFMLWGLPISIIGARLYYVLFDLPQYIADPIQIFNIRSGGLAIYGGLIAGAI
                     TLYFFTKYHFISLWTFLDIAAPSVLLAQAIGRWGNFMNHEAYGPDTTRSFLESLHLPQ
                     FIIDNMYIEGIYRQPTFLYESLWSLIGFILLLFLRKKPHLLKKGEVLLCYLMWYSFGR
                     FFIEGLRTDSLYFLGVIRISQLLSAVLFVGTLILFIWRRKKGNLPDYDRSLGKNQQII
                     "
     gene            19288..20313
                     /locus_tag="SCJ86_000562"
     CDS             19288..20313
                     /locus_tag="SCJ86_000562"
                     /EC_number="1.1.1.94"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002363959.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent glycerol-3-phosphate
                     dehydrogenase"
                     /protein_id="HEG1168148.1"
                     /translation="MNYRVAVLGPGSWGTALAQVLAENGHDVRIWGHRASQVEEINTQ
                     HTNQRYLPDHYLPDSIQAFTNMEDAVKDADAVLFVIPTKAIRSVAKELVPKLNTKPVI
                     IHASKGLEQGTHKRISDILLEEIPAEKRKAVVVLSGPSHAEEVAKHDITTITAASTDA
                     KAAKYVQELFMNNYFRIYTNPDVIGVEMGAALKNIIAIGAGAIHGLGFGDNAKAAIMT
                     RGLAEISRLGVAMGANPLTFIGLSGIGDLVVTCTSVHSRNWRAGNLLGQGQPLEEVLA
                     NMGMVVEGVNTTQAAMELAQMMEIEMPITQTIYEVLYEQKEIKTAAKEIMLRDGKKEN
                     EFSLTIF"
     gene            20328..21266
                     /gene="galU"
                     /locus_tag="SCJ86_000563"
     CDS             20328..21266
                     /gene="galU"
                     /locus_tag="SCJ86_000563"
                     /EC_number="2.7.7.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289574.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UTP--glucose-1-phosphate uridylyltransferase
                     GalU"
                     /protein_id="HEG1168149.1"
                     /translation="MKVKKAVIPAAGLGTRFLPATKAMAKEMLPIVDKPTIQFIVEEA
                     LKSGIEDILIVTGKAKRPIEDHFDSNIELETNLSEKGKTELLKLVEETTDVNLHFIRQ
                     SHPKGLGHAVLQARAFIGNEPFVVMLGDDIMEDEVPLTQQLIDDYERTHASTIAVMQV
                     PHDETSKYGIINPGEVLEDGLYNVKNFVEKPDPSKAPSDLAIIGRYLLTPEIFDVLSE
                     QQPGAGNEIQLTDAIDTLNKTQRVFARKFTGKRYDVGDKFGFMKTSIEYGLTHPEVGA
                     PLREYILSLGKTLEAEGSKIDHNPKGITEIKEEKKK"
     gene            21391..21831
                     /locus_tag="SCJ86_000564"
     CDS             21391..21831
                     /locus_tag="SCJ86_000564"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335987.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF948 domain-containing protein"
                     /protein_id="HEG1168150.1"
                     /translation="MSGGEIAGLIAAIAFAILVVFIVRVLLQVSKTLEKVDQTVAEAN
                     TTIEIVTKDVDLLSRQVEGLLVKSNELLLDINGKVATIDPLFTAVADLSESVSELNHS
                     SKNIATKVGGIGKTTAKATVAGKVGGTAMKFFKNKKHTETTGGK"
     gene            21834..22436
                     /locus_tag="SCJ86_000565"
     CDS             21834..22436
                     /locus_tag="SCJ86_000565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341623.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YtxH domain-containing protein"
                     /protein_id="HEG1168151.1"
                     /translation="MAKKGGFLLGAVVGGTAAAIAALLLAPKSGKELRDELAAQADDL
                     MDMASDYTDMAKEKSTELTGIAKEKAQDLSQQAGDLAGSVKQKVSESADEAGSESDDI
                     LLNLKEQSADLSNRFKKTAEDMKKPADELGSIAKDTSEDIMIDVKDAAGKAGDTVNEG
                     MSEAKPVADDSKEMMKMAKEDAKEAKKEIKDTMTDSSTNH"
     gene            complement(22495..23601)
                     /locus_tag="SCJ86_000566"
     CDS             complement(22495..23601)
                     /locus_tag="SCJ86_000566"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337325.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase P family protein"
                     /protein_id="HEG1168152.1"
                     /translation="MNNVKINELRQWMAQHQVELAYISDPGHIAYFSGYESDPHERVL
                     ALFIPLEQDPFLFTPALEVEDAQNSSWAYDVRGYLDSENPWEIIGKELRARYGQPGNV
                     GIEKNALTVERFDAISDILTGTDSYTDLTSIIQRLQLIKTLEEKDKLIEAGKWADVAF
                     EIGFKAVKEGVTEQAIIAEIEYQLKKQGVSQMSFDTLVLAGANAASPHGTPGNTKISP
                     NELVLFDLGVVWNGYCSDATRTIAYQKPTDFQEKIYNITLEAQLAAQEAVRPGVTAGE
                     LDQIARNVINSYGYGEYFNHRLGHGIGTTVHEFPSLVEGNDLVIEEGMCFSLEPGIYI
                     PEKVGVRIEDCVYVTSDGCVPFTTTPKELLVLEK"
     gene            23811..24812
                     /gene="ccpA"
                     /locus_tag="SCJ86_000567"
     CDS             23811..24812
                     /gene="ccpA"
                     /locus_tag="SCJ86_000567"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="catabolite control protein A"
                     /protein_id="HEG1168153.1"
                     /translation="MEKQTITIYDVAREANVSMATVSRVVNGNPNVKPATRKKVLEVI
                     DRLDYRPNAVARGLASKKTTTVGVIIPDVSNMFFASLARGIDDVATMYKYNIILANSD
                     GNDQKEVNVLNNLLAKQVDGVIFMGHHITDEIRGEFSRSKTPVVLAGSIDPDEQVGSV
                     NIDYVSATKDAVNKLAKSGNKKIAFVSGALIDPINGQNRLKGYKEALSENNLPYSEGL
                     IFEAQYNFKDGLSLVDRIHNSGATAVYVSDDELAIGILDGLLDRGVKVPEEFEIITSN
                     NSLLTEVARPRLTSITQPLYDLGAVSMRLLTKMMNKEEVEEKTIILPYGIEEKGSTK"
     gene            complement(25149..27494)
                     /locus_tag="SCJ86_000568"
     CDS             complement(25149..27494)
                     /locus_tag="SCJ86_000568"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300682.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding protein"
                     /protein_id="HEG1168154.1"
                     /translation="MPRKNTRKKQNRRKKEKWFVPKIIFRVFQSLTVFITVLLLMFAA
                     LGIGIGAGYFAYLVEDTQLPTKKALQTELGNITETSKIVYADNTEISKIQTDLMRTTI
                     SSDKISPLLKTAIISTEDEYFDKHQGYVPKAVLRALVSEATGIGSSGGSTLTQQLVKQ
                     QILTDETTFKRKANEIILSAQVEKYFSKDEIIATYLNVSPFGRNNKGQNIAGVQEAAK
                     GIFGVDAKDVTLPQAAYIAGLPQSPITYSPYTNTGALKEDLSAGLARKDFVLFSMYRE
                     GQITKEQYEEAKAYDLTKDFLPQQIAEQTDREFLYYTVMNEATRIIAQQLAEKDNADM
                     SDSSVSDAYYQKAQQTIQNKGYTIHSTIDKDVYAALQNGVENYGYMLDDGAGSQVETG
                     NVLMDNRTGRIYGFVGGRNYSQNQNNHAFDTERQAGSSIKPVLVYGPAIDMGLIGSES
                     RVSDYATTWQEGENAGEKIVNATNEGSNTFQTVRESLEWSNNIPAYHLYQDVLNSGGS
                     KQYAYEHYLAKMNYPANDNWGVESAPLGTVDVTTLQQTNGFQALANGGVYEEGYIIDS
                     ITDNAGNVIYKHESNSVRIYSEATASIMNDMMRSVINAKITTPFKDAISSLNGNLGKA
                     DWVGKTGSTNEYRDSWLIVSTPSITISSWAGHDDNTGMDSKARIRSANYLANLINQAY
                     QAKPDIFGTDEKFELSSDVMKKKVSGFTGQTPGKVTVNKKSIQTPGKTVESLWAKNGP
                     KKSAFKFGVGGTDENYTDYWNTASAYARANQATKESDEKDD"
     gene            27773..28675
                     /locus_tag="SCJ86_000569"
     CDS             27773..28675
                     /locus_tag="SCJ86_000569"
                     /EC_number="3.1.21.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334132.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="deoxyribonuclease IV"
                     /protein_id="HEG1168155.1"
                     /translation="MLLGSHVSMSGKKMLLGAAEEAASYGSSTFMIYTGAPQNTRRKA
                     IEEMNIDAGKAYMAEKGLTNIVVHAPYIINLGNTIKPEMFPFAVQFLREEIVRAEALG
                     ATQITLHPGAHVGAGTEAGIARIIEGLNEVLTKEQTAQIALETMAGKGTEIGRTFEEL
                     AQIIDGVKLNEKLSVTFDTCHTNDAGYNIKEDFSGVMEEFDKIIGLDRLKVLHINDSK
                     NPQGSHKDRHANIGFGTIGFDTLNKIVHDPMFDNISKILETPYVGKDKKKAYAPYGKE
                     IAMFKAQTFDPEVFPELLAEEVIL"
     gene            28888..30663
                     /locus_tag="SCJ86_000570"
     CDS             28888..30663
                     /locus_tag="SCJ86_000570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291668.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucosyltransferase"
                     /protein_id="HEG1168156.1"
                     /translation="MLNTLKPLINQLKKKHTYFILGIALLFTIIRLYLYSKAIYGTDM
                     SATYDDQLLIRYAQNILDGKWLGEYTTTTLSKGISYSLFLVLAHKLHLSYAVLFGMFS
                     IFASILVALAFKPIVKNKYLLLSIYFYFLFSPISFTHEYSTRTYRNALVIPAVEIIIA
                     CLLAIYYRKYGSSRKLLPWFLGLSVAFPFFWFIREDSIWLLPFTVVALGISFVQILLG
                     QDFSFKEVKKLKKRLFLLSKREWLKLSLFLLPIVGFSIVYQWIEYKNETTYGIATIND
                     RSSGEFGRLMKQLIRIDDETNLNKENSEIWVSHAALNKALAASPTFHTVSTRIDELYT
                     THAWTQGGKVKELPGDIIFWALRDVVSESGYYQNNAKETNDFWKKVNQELEEAYHKGT
                     IKKKKEFYLMGSGDGKTMSDLPIVFDFLQSGYAYAWRYKDFHQGGNYSFGTQEQVKQA
                     QELLNLPLMENWIDSNEPRTPNFQLTKSAKVANVIIKIYQVLAIPVLIASIAGILLMI
                     LGTVFAKADRAYYSSSLIVVTGLILTQLVFLFGVSWFCSYAPEKKDYFLSVYTGAGVP
                     LTQFIVVTSMLVFSKLNLKKALSSK"
     gene            complement(30729..32315)
                     /locus_tag="SCJ86_000571"
     CDS             complement(30729..32315)
                     /locus_tag="SCJ86_000571"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377431.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Nramp family divalent metal transporter"
                     /protein_id="HEG1168157.1"
                     /translation="MNNQSNKQFHKHRLIEHTNGLSLAEVNGTVDVPKGKSFFKTLLA
                     YSGPGALVAVGYMDPGNWSTSITGGQNFQYLLMSVILMSSLIAMLLQYMAAKLGIVSQ
                     MDLAQAIRARTSKTLGVILWILTELAIMATDIAEVIGAAIALYLLFDIPLVIAVFITV
                     LDVFVLLLLTKVGFRKIEALVVCLIFVILFVFVYQVGLSNPDWHSLFAGFIPNSHTFS
                     DSPVIGGETPLTGALGIIGATVMPHNLYLHSAVSQTRKIDHQNEEDVAQAVRFSTWDS
                     NIQLSMAFLVNALLLIMGVAVFKTGSVQDPSFFGLYEALSDPSAMSNGILIAVAKSGI
                     LSTLFAVALLASGQNSTITGTLTGQVIMEGFIHMRIPIWLRRLVTRLLSVIPVLICVL
                     FTSTKGTIEEHAALNNLMNESQVFLAFALPFSMIPLLMMTNSQAEMGSRFKNSFIVKL
                     LGWFSVISLTYLNLRGLPGQIESFFGEQATSAQLALADHIAYVIIVLVLLLLAWTIGD
                     LYKGNKKYEQLLQETVEEIE"
     gene            32566..32790
                     /locus_tag="SCJ86_000572"
     CDS             32566..32790
                     /locus_tag="SCJ86_000572"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291670.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YneF family protein"
                     /protein_id="HEG1168158.1"
                     /translation="MLSTGIVVLIAIIALLIGAVGGFFLARKYMKDYLEKNPPVNEDM
                     LRSMMMSMGQKPSEKKIRQMMQQMKNQGKK"
     gene            32932..34683
                     /locus_tag="SCJ86_000573"
     CDS             32932..34683
                     /locus_tag="SCJ86_000573"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304603.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HEG1168159.1"
                     /translation="MSIFRKLGWFFRQEKKHYLIGVFSLFAVALVQLVPPKVIGIIID
                     EIADKNLSIKIIFGWIFLLIIAAFLQYLFRYIWRTNIWGSAARLEKELRQQLFKHFTE
                     MDNLFYQKYRTGDLMAHATNDLNAIQNVAGAGILTFADSVITGGTTIIAMILFIDWRL
                     TLIALIPLPLLAVTSRVLGSKLHDAFRDSQEAFSSINDKTQESITGIKVIKTFGQEKE
                     DLADFEEKIEDAIIKNKRVNFLDSLFDPFITLIIGLSYVATIILGGKYVLEGTISIGQ
                     MVSFISYIGMLVWPMFAIGRLFNVLERGNASYDRVNELLHEKTHIVEQLGAVQTPAKG
                     ELNVAISSFNYPKEESPALEQIKFAIQEGETLGIVGKTGAGKTTIIKLLMREYDDYQG
                     KIQFGGRSIKEYSLNALLGSIGYVPQDHFLFSMTIRDNIRFSNPKLSEEEVEHAASLA
                     FINNEIKELPKGYDTLVGERGVSLSGGQKQRISIARALIKNPELLILDDALSAVDAKT
                     EEAILHNLKEIRKEKTTIIAAHRLSSVMHAKEIIVVDQGKIIERGTHHELLALGGWYE
                     QMWEKQQLEAKIEGGEA"
     gene            34683..36467
                     /locus_tag="SCJ86_000574"
     CDS             34683..36467
                     /locus_tag="SCJ86_000574"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338974.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEG1168160.1"
                     /translation="MDENYQSEWTKSIPLKEQKKIIKRLMKFAKPFRRTFIVAILFAF
                     ALSVINVLLPRIIQTFMDDHLAKQSATTQVILFFAGMYLFGVIVKSIIWFFQWYLYSM
                     ASLKTYQYIRVKLFEKLHTLGMRYFDQTPAGSTVSRVTNDTETLFEFWYVFLMVLTGI
                     FAVVSSFLAMFQINGEIALWTLVFLPILGIVIWYYQKFSSRIYRNMREKLSQLNTKLN
                     ESISGMRIIQQFRQEARLAKEFEQVNNEYLDTRYAMIKTNSILLSPIINFLYALAIAL
                     VLTLFGIDALHSPVEVGLIYAFTTYVQGFFNPMSQMMDFLSIFTDGMVAGSRILKIMD
                     TEEYAPQQNPGADQMITSGKIEFRNVSFSYDGEHNVLNNISFVANPGETVALVGHTGS
                     GKSSIINVLMRFYEFYEGQILIDDHDIREYPMEELRKKMGLVLQDAFLFYGDIAGNIR
                     MMNPAITDEQIRHAAEFVQANKFIDELPKGYHSKVIEHGASYSSGQRQLITFARTIVT
                     DPKILVLDEATANIDTETEGLIQEGLAKMRQGRTTIAIAHRLSTIRDAELILVLDKGH
                     IVERGNHETLLAQNGLYADMYKLQSSGI"
     gene            36793..37644
                     /locus_tag="SCJ86_000575"
     CDS             36793..37644
                     /locus_tag="SCJ86_000575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289170.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PRD domain-containing protein"
                     /protein_id="HEG1168161.1"
                     /translation="MLIEKVLNNNVVITRDERNQEMIVMGKGLAFKKKVGDPIFDSCI
                     DKKFQLTNDGLSQQFQELAQAIPLEYLEISCEIIEYTKEALQVQLNDSVYIALTDHLY
                     TAIERHKMGIFVPNVLLYDIKQLFPKEYAIGKKTVKKISDKYHIKLSEDEAGFIALHL
                     VNAQGIQSQQDNTYLFTITIQDILNIVRYYYKITFDEDSVHFFRFVTHLRFFLTRVLD
                     GEKSEVGEVSEELFQVIQKKYKDTFGCVEKIADFLAETFNYQMSTDEKMYLTIHTARL
                     VHENRCK"
     gene            37698..39449
                     /locus_tag="SCJ86_000576"
     CDS             37698..39449
                     /locus_tag="SCJ86_000576"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311278.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucose transporter subunit IIA"
                     /protein_id="HEG1168162.1"
                     /translation="MAKPSTNKLEGIEMGKYQELARKIVENVGGKENINGLTHCITRL
                     RFKLKNEEKANDEILKNMDGIVTVMRAGGQYQVVIGNHVPVVFEEVIKAGNLTFDEAV
                     STEKMRPFDMLIDGISGCFQPFLAILAAGGMIRGLTAFLVFLGAFDRGSGTFVMFDNI
                     GDSVFQFMPVIIGLTAARKFKVNEFVGMLLGAALMNPSLSLEALSGAAEAPLTTIFSG
                     TIFEAPIYQTVFGIPWIARNYASSVIPIIFIVLLASQIQKPIKKLVPEMIANFFVPFF
                     TVLITMPLGFLLVGPVFTFATDILMAGFETLLALSPVIYGAIVGFFWQILVMFGLHWA
                     IVPMGLMQFSVNGWQNIMTPVAVVSFGQTAALTALYFKLRNPKDKAIAIPAIVSGIVG
                     ITEPAIYGFTLPRKKIFIFTCVGGALGGAYSGLMNLTSWNQGGLGIFTIPNYIRPDGD
                     LTDVINVLIGIAIAMVVSFTLTFFFWKDEAGETDDIQKKSGKEIVKTPIQGQIAPLNA
                     AKDAAFAQGTLGRGILIYPEKGEVRAPFDGTVMTLFPTKQAIGMVSETGLELLIHVGL
                     DTVQLEGKYFESLVQQG"
     gene            39453..39623
                     /locus_tag="SCJ86_000577"
     CDS             39453..39623
                     /locus_tag="SCJ86_000577"
                     /inference="COORDINATES: protein motif:HMM:NF012578.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucose transporter subunit IIA"
                     /protein_id="HEG1168163.1"
                     /translation="MKQGDLLLRFDKKKIEKEGYSLETPVIVTNYTDYLDILENQGET
                     VGPDDALITALT"
     gene            complement(39663..40565)
                     /locus_tag="SCJ86_000578"
     CDS             complement(39663..40565)
                     /locus_tag="SCJ86_000578"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334136.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MurR/RpiR family transcriptional regulator"
                     /protein_id="HEG1168164.1"
                     /translation="MLLREKIKEISFSPSENEVIQFLLSDSPALGEMTIQAIAERCYV
                     HPSTLIRVAKKMGFDGWVSFRNAFLAETAYLEGNFQDVDANLPFSTTDGIMTIANKMA
                     SLEQMTIHDTLSLIHHDQLQKAKQLMLQAKKIVIFASNSNTLISQDFMLKMKRIKMDV
                     TIVTTFGESFYEAYNCTSDMCGILISYTGENNVILRTAEILKQNHVPILALTSIGDST
                     LSAMSTAALRLTTRERLYSKIGNFTINTSICYLLDVLYSCVFAENYQQNLVHLISVGQ
                     KFDKRPISSAIMKEPFNFENKEVE"
     gene            40796..42199
                     /locus_tag="SCJ86_000579"
     CDS             40796..42199
                     /locus_tag="SCJ86_000579"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-phospho-beta-glucosidase"
                     /protein_id="HEG1168165.1"
                     /translation="MAKGIKIATIGGGSSYTPELMEGFIKRYEELPIREIWLVDVEEG
                     KEKLEIVSQMSQRMWDASPYEVKIYATLDREEALKDADFVTTQFRVGQLDARIKDERI
                     PSYYGMIGQETNGAGGIFKAFRTVPVVLAIVEDMKRLCPNAWLINFANPSGMVTEAVI
                     RYGKWDKVIGLCNVPVMAMMTEPAMLGKKKEELIYKFAGLNHFHWHKVADNKGNDRTA
                     ELIDRLYDEENGLPKNIFDIPFFKEQLKQMNMIPCGYHRYYYREEEMLEHALEEYQTI
                     GTRAQQVKETEAELFELYKDPELDHKPEQLQKRGGAYYSDAACETIASIYGNKETQIV
                     VSTKNDGAVPDLPADCVVEVTAYVGAQGARNVAFGSLPAAERGWLQVMKNMELLTIEA
                     AVTGDYGTALQAFTINPLIRSGKTAKRIMDELFIAHQQYLPQFKETISRLKEEGIEVQ
                     DELARELSGNTHIMEEV"
     gene            42199..43632
                     /locus_tag="SCJ86_000580"
     CDS             42199..43632
                     /locus_tag="SCJ86_000580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008264482.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-phospho-beta-glucosidase"
                     /protein_id="HEG1168166.1"
                     /translation="MGFRKDFLWGGATAANQCEGGYDEGGRGLANVDVVPIGKDRFPV
                     ITGKKKMFEFDEDHFYPAKIAIDMYHRYKEDIALFAEMGFKTYRMSIAWSRIFPMGDE
                     KEPNEAGLKFYEDAFKECQKHGIEPLVTITHFDCPMHLIKEYGAWRSRELVGFYENLC
                     RVIFNRYKGLVKYWLTFNEINMILHAPFMGAGLYFEKGENEEQIKYQAAHHELVASAI
                     ATKIAHEVDPENKVGCMLAAGAYYPYTCNPEDVWASRKSDRENYFFIDVQSRGEYPAY
                     ALKEMERNGIQIEMEPGDAALLKEHTVDFISFSYYASRVSTTDNELLEQTAGNIFASV
                     KNPYLQASEWGWQIDPLGLRITMNDIYDRYQKPLFIVENGLGAVDTPDENGYVEDDYR
                     IDYLAAHIQAMKDAVELDGVDLLGYTTWGCIDLVSAGTGEMKKRYGFIYVDRDNQGNG
                     TLARSKKKSFNWYKKVIDSNGENLSND"
     gene            43777..44703
                     /gene="trxB"
                     /locus_tag="SCJ86_000581"
     CDS             43777..44703
                     /gene="trxB"
                     /locus_tag="SCJ86_000581"
                     /EC_number="1.8.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin-disulfide reductase"
                     /protein_id="HEG1168167.1"
                     /translation="MYDVIVIGAGPAGMTAALYASRSNLSVLMIEQGAPGGQMNNTAE
                     IENYPGFDSIMGPDLAYKMYESVSRFGTENAYGIVQDIKDHGSHKEVICEDKSYEGKT
                     VIIATGCIHRKLGVPGEEEFAGRGVSYCAVCDGAFFRNKRLVVIGGGDSAVEEAIYLT
                     QFASEVVIVHRRDELRAQKIIQDRAFANEKISFMWDTVVDEIVGNEMVVTGVKARNVK
                     TGNEFDLAADGVFIYVGLDPLTAPFREAGLTNEAGWIPTDNEMKTNMPGVFAIGDVRE
                     KDLRQITTAVGDGGIAGQQVYKYLESLEETTA"
     gene            44917..46644
                     /locus_tag="SCJ86_000582"
     CDS             44917..46644
                     /locus_tag="SCJ86_000582"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005880351.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phospho-sugar mutase"
                     /protein_id="HEG1168168.1"
                     /translation="MSWEQVYEQWVHEENLEENLKKQLNDLGEDPEKLEDAFYAPLEF
                     GTAGMRGILGPGINRMNIYTVRQATEGLARFMDTQGEETKRRGVAIAYDSRHQSPEFA
                     MEAAKTLAHHNIPSYVFESLRPTPELSFAVRHLKTFAGIMVTASHNPAAYNGYKVYGE
                     DGGQMPPADADALTKYVREVSNPLKVEVLSDEEAKHSGLITIIGEEVDAAYLEEIKAV
                     TIDRELVETMGKDLKLVYTPLHGTGKMLGERALKQAGFEQFVLVPEQAVADPDFTTVK
                     SPNPEEHSAFEYAIRLGEKEGADLLIATDPDADRLGAAVRLPDGSYEVLTGNQIGALF
                     AQYILEAHQSRGTLPENAAVLKSIVSSELPTAIAEHYGATMFNVLTGFKFIAEKIQQF
                     EEDHSHTFMFGFEESYGYLVKPFVRDKDAIQALLLLAEVAAYYKKQGKTLYDGLQTIF
                     ETYGYFAEKTISVTMSGQEGSAKISALMEKFRKEAPTEFAGAKVIQTEDFKELTRTDR
                     EGNVEKLTTPPSDVLKYTLEENGWIAIRPSGTEPKIKFYIGVKGSSTEDAAEKVASYE
                     AIIKKMTEE"
     gene            46659..47009
                     /locus_tag="SCJ86_000583"
     CDS             46659..47009
                     /locus_tag="SCJ86_000583"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325829.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="winged helix-turn-helix transcriptional
                     regulator"
                     /protein_id="HEG1168169.1"
                     /translation="MEKVVNFRSQSFFKLEVNGLPDTQKSKVEKVSQLFKMLSDPTRL
                     KILLYLKDGEQNVTAITQAVEMEQSAVSHQLRLLRENHVVKSHREGKAILYSLDDSHV
                     LDILNQTLKHVEHQ"
     gene            complement(47101..47961)
                     /locus_tag="SCJ86_000584"
     CDS             complement(47101..47961)
                     /locus_tag="SCJ86_000584"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016632125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="kinase/pyrophosphorylase"
                     /protein_id="HEG1168170.1"
                     /translation="MSSDSTEIVTFFVISDSAGETATKLAQATMAQYPTVEFNLFRRT
                     FVTDEKTLRQALQDALEEKALVLHTLINQELIEITRSFCEEHQLFSFDVMTPPISEIE
                     RLTGIKPIRQPGALHLLNENYFKRIKAMEFAVKYDDGKDPRGFLEADVVLLGVSRTSK
                     TPLSLFLANKNLKVANLPLIPQAHIPKQLWEIDPKKIVGLTNNPDILNNIRKERMRSY
                     GLNPDTAYSDIEKIRAELDFANDLYEKLGCVVINVASLSIEETASLILNALDLEDHSY
                     YGTETTEKSD"
     gene            48053..48481
                     /locus_tag="SCJ86_000585"
     CDS             48053..48481
                     /locus_tag="SCJ86_000585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319219.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional repressor"
                     /protein_id="HEG1168171.1"
                     /translation="MKKQTLIEQSLETLKNNGFKYTKKREALLTYLVKRNRYVSAKEV
                     FDYMNQEFKGVSYDTIYRNLHDFAEIDLLEETDLNGEMKFRFHCCQGEIGHHHHFICT
                     VCGTTKEIHMCPMNYFEEQLGGCTIEGHRFEIFGRCENCQ"
     gene            48643..48819
                     /locus_tag="SCJ86_000586"
     CDS             48643..48819
                     /locus_tag="SCJ86_000586"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010754017.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S21"
                     /protein_id="HEG1168172.1"
                     /translation="MSKTVVRKNESLDDALRRFKRSVSKAGTLQESRKREFYEKPSVR
                     RKKKSEAARKRKKF"
     gene            48846..49292
                     /locus_tag="SCJ86_000587"
     CDS             48846..49292
                     /locus_tag="SCJ86_000587"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734957.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GatB/YqeY domain-containing protein"
                     /protein_id="HEG1168173.1"
                     /translation="MSLLSRLNDDMKAAMKAKDKESLQVIRMIKSSIQNEQIKEGHDL
                     TEEEELTVLSREMKQRRDSLHEFEEAGRDDLAEKVKSEIVIVEKYMPEQLSDEEIRQL
                     VQEAITQTGASSMKEFGKVMGAIMPKVKGKADGNQVNAIVKELLQS"
     gene            complement(49446..50825)
                     /gene="brnQ"
                     /locus_tag="SCJ86_000588"
     CDS             complement(49446..50825)
                     /gene="brnQ"
                     /locus_tag="SCJ86_000588"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629172.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid transport system II
                     carrier protein"
                     /protein_id="HEG1168174.1"
                     /translation="MEETKTYSWKQLLMVSSLIFGMFFGSGNLIFPVHLGQMAGSNWI
                     SAGVGFAISGALFPLLAILAVVVTKSDGLYDLAKPVGKWYAALFLILVHLTIGPFFGT
                     PRTAATAFEMAAKPFLPEKYDSLGMFLFSALFFLLAYFLTVHPTRLVKYVGKYLNSLF
                     LALLAIIFVVSLFQPMGNLNQSAADSYQTNALTNGVLEGYNTVDAVALLALSVTFVHA
                     VRDLGFKDKKVTELTAKAGSLSVALEVMIYFGLVLLGAMSLNKMPLSENGGTALSQIT
                     NFYLGNFGASFLGVMVTLGVFTTAMGLVVSFAQDFHKLFPKVSYMTWLRLTTFVSFVV
                     ANAGLDNIIQWSLPVLMLLYPLSLALILLSLTAKFFQKSPFVYQVTMFFSAVPAVLDM
                     LANSPALVSQQTVVASLLGFYHHHVPFAALGLGWMVPTLLGYAGSLLFYYAYRLSGYK
                     QEANELPEE"
     gene            51531..52502
                     /locus_tag="SCJ86_000589"
     CDS             51531..52502
                     /locus_tag="SCJ86_000589"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631377.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PhoH family protein"
                     /protein_id="HEG1168175.1"
                     /translation="MTEESSSLEIRLTAADDVSMLLGAHDKHIKLIEETTETTIHTRG
                     EIIQIIGEQSQISLAASVIRTLQELIKRGIHVSTPDVVTALKMGQKGTLDYFVEMYEE
                     EIVKDRNGKPIRVKNSGQKKYVESVAKHDIVFGVGPAGTGKTFLAVVLAIAALKKGEV
                     QKIILTRPAVEAGENLGFLPGDLKEKVDPYLRPVYDALYQIFGLDHTNRLMERGVIEI
                     APLAYMRGRTLDDAFVILDEAQNTTVAQMKMFLTRLGYQSKMIVNGDTSQIDLPRGTT
                     SGLVHAERTLKQIKKIDFVNFEASDVVRHPVVAEIIKAYEKADLHQE"
     gene            52526..54718
                     /locus_tag="SCJ86_000590"
     CDS             52526..54718
                     /locus_tag="SCJ86_000590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313518.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HDIG domain-containing protein"
                     /protein_id="HEG1168176.1"
                     /translation="MLNKTLQKLQEKLGKAALPFLLFLFFIIICGVTFSSVKQKANDF
                     REGQVAEESIRANKTIENTEETEQKRKLAAEAVTPEYTYQKDLADDQNNRIKQLFELI
                     DKTNDAINKSYDEKIDKAKEDENIPKPTTEERIAALKKNFENVNAENVAFYQKLPNNF
                     YSTIFEMTETEIHTVRDESLKLIDEQMSKQVRESELEAFKQEAEEQIQYLNVTPAQQQ
                     MIRYLVDQGIVVNDVLNEKKTEELKQSAREAVQPVMIFQGEIIVREGNQIDASAMKKL
                     ELLGLTNQTTSIFPLIAMVLAVLLQIGVLIYNSLQFQNAGERTKYVLFYVTAMSVSIV
                     LMKFFQLFQTEQATYIPLFYPAAFAPLVLSFFLNRRAGIMAGIFQAVSALFIFYDSIG
                     TNFLTIILMSYVFSGLMATIVRRKRISEQGLSAAMWVIIFPVMMDVILIVYQGMSFGD
                     ATTWLMLVCGFTGALFSFLLTIGLHPYIELMVNDDSVIVLNELSNPNHPLLKQLLEEA
                     PGTYHHSMMVANLSANAVAEIGGRSLLTRVACYYHDIGKIKHASFFVENLPSGAENPH
                     NFLLPEDSKHIIFGHVTDGAKILEDYNMPQMVIDICKQHHGTTLMRYFYIKAKERNPE
                     VTEEQFRYPGPKPQSREAGIVNIADSCEAAVRAMDHPTSEKIESFVHNLIEERISDGQ
                     LDDSGLTLKEIRKVEKSLISGLSSTFHSRIKYPKMKTEVEKIKEEQEE"
     gene            54735..55211
                     /gene="ybeY"
                     /locus_tag="SCJ86_000591"
     CDS             54735..55211
                     /gene="ybeY"
                     /locus_tag="SCJ86_000591"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322767.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rRNA maturation RNase YbeY"
                     /protein_id="HEG1168177.1"
                     /translation="MDITFMDETNQVSEEKMKEIDDLLQFAASYLKLPEETEMSVTFM
                     DNAAIQVINRDYRGKDAPTDVISFALEEEGEDEIPVIFEDGENPLPRVLGDIMISIER
                     AAEQAEAYGHSYDRELGFLAVHGFLHINGYDHMTPEDEKEMFGLQKEILDAYGLKR"
     gene            55195..55590
                     /locus_tag="SCJ86_000592"
     CDS             55195..55590
                     /locus_tag="SCJ86_000592"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diacylglycerol kinase family protein"
                     /protein_id="HEG1168178.1"
                     /translation="MDSKDKNVEKNKHFLMSVEFAVQGVKTVFEEERNMRKHVAFGIL
                     ALFMGVIFQLDRIEWLWLLLAVFLVWIVEIINTVFENVVDMFTDFHFHPIGKKIKDMA
                     AGAVLVTACFAVIIGLILFVPKIYHLLFN"
     gene            55605..56504
                     /gene="era"
                     /locus_tag="SCJ86_000593"
     CDS             55605..56504
                     /gene="era"
                     /locus_tag="SCJ86_000593"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016625712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase Era"
                     /protein_id="HEG1168179.1"
                     /translation="MPEHKSGFVAIVGRPNVGKSTLLNRIVGQKIAIMSDKAQTTRNK
                     IQGVYTTPEAQLIFIDTPGIHKPKHRLGDFMVEAAYSALKEVDAVLFMISADQKRGRG
                     DDFIIERLKNVHSPVYLIINKIDKIHPDELLGIIEDYSTQMPFAQVIPISATEGNNVE
                     RLMDVLVSEMPEGPQYFPDDQVTDHPEYFIVSELIREKVLFLTRDEVPHSVAVVVDSM
                     KRNENDKIHIQATIIVERDSQKGIIIGKGGKMLKQVGTKARKDIENLLGDKVFLELWV
                     KVQKDWRDKKVYLQDFGYRKDEY"
     gene            56647..57459
                     /gene="recO"
                     /locus_tag="SCJ86_000594"
     CDS             56647..57459
                     /gene="recO"
                     /locus_tag="SCJ86_000594"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347340.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RecO"
                     /protein_id="HEG1168180.1"
                     /translation="MSQLAESKGLILSSRNYKEKDKLVKIFTESSGKMMFYIKGIHRQ
                     NQPLAPALLPFTEAVYIGNFRSEGLSFLSSVKDVHAFRNIQEDIFLSGYGTYLLNLVD
                     AAIEDHQYDPHLFQFTQQALQRMDQGDDAEIITNIFEVQILQRFGIAPIWTHCVVCGE
                     TKGKFDYSSKSGGVICEKHWPMDEHRYHADPRAVYFVRMFSAISYDKISGIHLKEETK
                     QAIRDLIDQLYDEYVGIHLKSKKFIDQMKSWAHVLKTPEKNMPEKEIPEKEE"
     gene            57843..58760
                     /gene="glyQ"
                     /locus_tag="SCJ86_000595"
     CDS             57843..58760
                     /gene="glyQ"
                     /locus_tag="SCJ86_000595"
                     /EC_number="6.1.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340165.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine--tRNA ligase subunit alpha"
                     /protein_id="HEG1168181.1"
                     /translation="MSKKLTVQDMILTLQKFWSSNGCMLMQAYDTEKGAGTMSPYTFL
                     RAIGPEPWNAAYVEPSRRPADGRYGENPNRLYQHHQFQVVMKPSPENIQELYLESLRL
                     LGIDPLEHDIRFVEDNWENPSMGCAGVGWEVWLDGMEITQFTYFQQVGGLPCKPVTSE
                     ITYGLERLASYIQEVESVYDLEWADGVKYGEIFKQPEYEHSKYSFEVSDQDLLLSNFD
                     RFEKEAKRCIDENLVHPAYDYILKCSHTFNLLDARGAVSVTERAGYLSRIRNMARAVA
                     KIFVAEREKLGFPLLNKNTANPENMKEAE"
     gene            58762..60837
                     /locus_tag="SCJ86_000596"
     CDS             58762..60837
                     /locus_tag="SCJ86_000596"
                     /EC_number="6.1.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010728817.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine--tRNA ligase subunit beta"
                     /protein_id="HEG1168182.1"
                     /translation="MAKDLLLEIGLEEMPAHVVTPSMKQLEEKTAKFLDENQLTYDTI
                     ETFSTPRRLAIKVTNIPERQADSEEEVKGPAKKIALDADGNWTKAAEGFVRGQGLTTD
                     AITFRELKGVEYVYVTKYTHGKEAKEVLTGLADVIKSLTFPVTMHWAEYDFEYIRPIH
                     WIVALLDDEVIPFEILDVKTGRASRGHRFLGEDVTFAHAKEYEEKLNEQFVIASPQKR
                     QEMIVTQANELAEKNHWVIDLDEDLLEEVNNLVEYPTVFSGDFEEKYLSVPEEVLVTS
                     MKEHQRYFEVRNDQGMLLPHFISARNGDRVKLENVVRGNQKVLTARLEDAEFFYNEDK
                     KASIDQYVEKLKTVTFHEKIGSIYEKMQRVQVIARIIGEQVGLSEEELMDLQRAAEIY
                     KFDLVTNMVGEFPELQGIMGEKYALLKGEKPAVATAIREHYMPISSEGELPQSTVGSV
                     LAIADKLDSIFSFFAVGMIPSGSNDPYALRRQAYGVVRIIESKKWAFPLSVLQETISD
                     VISKDTDRFGIGLSEGQQQVIDFIKGRLRQLLTTKNIRHDVIEAVLNAEQKDLTKVFA
                     AAQLFKQHLADEDFKPSMEALTRVVNLAKKAELEKQSEVDPELFENEAEKELHKAVEA
                     MKKSFADQTINENYDQLVSLRPLIENYFDQTMVMAEEPAVRKNRLTQLSQIAAMALSL
                     ASLDQIITK"
BASE COUNT        19863 a        10760 c        13027 g        17225 t
ORIGIN      
        1 aaaacttgca gcagctactg acagctgccg caagtttttt tgagtttcct cctcttttag
       61 atgaaatatg aataaattat gaagttctta ctttaatacg cttaagatat ttcaattatc
      121 tataactaat gataaaatga aaagtaaagt ctacatagtc tggataatac acagaaggaa
      181 aaggagatta cacaacgcat ggccaatttg attcgttcac ttatcgaaaa cgataagaaa
      241 gaactaaaac gtttagatag cattgcaaaa aaagttgaag catacgctga ccaaatggca
      301 gcattaactg atgaacaatt acaggcaaaa actgctgaat tcaagggcag ataccaaaaa
      361 ggagaaacat tagatcaatt acttccggaa gcttttgcag tcgtccgaga agcagcaaaa
      421 cgtgtattgg ggttatatcc acaccacgta caattgatgg gtggaatcgt gcttcacgat
      481 ggaaatatcc ctgaaatgcg tactggtgaa ggtaaaacct tgaccgcaac gatgccagta
      541 tatctaaatg ctctgactgg cgaaggggta cacgtagtta ccgtcaatga atatttagca
      601 acccgtgact cgacggaaat gggcgaactt tataatttct taggattaac agtggggttg
      661 aatatcaact caaaatcttc ggatgaaaaa cgcgaggctt ataattgtga tatcacttat
      721 agtacgaata atgaattagg atttgactac ttgagagaca atatggtcgt ttaccgccat
      781 caaatggtac aacgtccgct gaattatgcc attgtcgatg aagttgactc aatcttgatt
      841 gatgaagcga gaacgccgct gatcatttca gggcaagcag aaaaatccac agctttatat
      901 acaagagcag acaacttcgt gaaacgtttg aaagaagaag aagattataa aatcgatgta
      961 caatcaaaaa caatcagttt aaccgaagca gggatcgaaa aagcagaaga aacattcggt
     1021 ttggataact tatacgacat tgaaaatact gcattgaccc atcacttgga tcaagcattg
     1081 agagccaact atatcatgct tttagatatc gattatgttg tccaagacgg aaaagtactg
     1141 attgttgacc agttcactgg tcggatcatg gatggacgcc gttattcaga tggacttcac
     1201 caagcaatcg aagctaaaga aggcgtagag atcgaagacg aaacgaaaac catggcgacg
     1261 atcactttcc agaactattt ccgtatgtac aagaaacttg cgggaatgac tggtacagct
     1321 aaaacagaag aagaagaatt cagagaaatt tataacatcc aagtatacca gattccgaca
     1381 aatcgtccgg ttatccgtga tgatcgtcca gatttgcttt acccaacatt acaaagcaag
     1441 ttccacgctg tcgtgcaaga tatcaaagat cgctaccgca aaggtcaacc tgtgttagtc
     1501 ggaactgttg cggttgaaac atctgagtta ttatcagata tgctgaatca agaacgtatt
     1561 cctcatgaag tgctgaatgc gaaaaatcac tttaaagaag cagagatcat catgaatgct
     1621 ggtcaaaaag gtgctgtgac gattgctacc aacatggctg gtcggggaac agatatcaag
     1681 ttaggacttg gggtaagaga acttggtggt ctagcagtta tcgggacaga acgacatgaa
     1741 tctcgtcgta tcgataacca gcttcgagga cgtgcaggac gtcaaggaga ccctggtatg
     1801 tcacagttct acttgtcatt ggaagatgat ttgatgaaac gattcggttc agaacgaatc
     1861 aaatctttct tagatcggat gaagatcggt gaggaagatg ctgttatcca aagcaagatg
     1921 ctgacgaaac aagtagaatc tgcacaaaaa cgtgtagaag gaaataacta tgacacacgt
     1981 aaaaacgtct tacaatacga tgatgtgatg cgtgaacaac gtgaagtaat ctataaacag
     2041 cgacaagaag taatcatgga agaaaaaagc ttatccaagc cactaatgaa catggtcaaa
     2101 cggacaatca gtcgtgtagt agatgcgcat acacaattag agaaagaaaa ttggaatttg
     2161 gaaagtatca tcgattttgc cggcagtact cttgtccatg aagatagtat tactcttgca
     2221 gatatcgaag gaaaaacagc tcaggagatc aaagagtatc taaacgaacg agcacaagaa
     2281 gtatttaata ccaaagcggc tcaactaaat ggtccagagc aactgttgga atttgaaaaa
     2341 gtcgtgatct tgcgtgttgt ggactctaaa tggacagacc atattgatgc aatggatcaa
     2401 ttacgtcagt caatcggctt acgtgcttat ggacagaaca atccattagt cgaatatcaa
     2461 acagaaggct ataagatgtt tgaagatatg gtaggcgcca tcgagtatga agtcactcga
     2521 atctttatga aagctgagat ccgtcaaaac gtacagcgtg aacaggtttc tcaaggaaca
     2581 gcttctcact cagaagacgg agatgccaaa gaaaatagta atactagcgc gaaaaaacag
     2641 ccaatccatg tgaacaaggt cggaagaaat gatccttgtc cttgtggaag cgggaaaaaa
     2701 tataagaact gtcatggaaa aaatgcaaac taatttctga cagcaggatt ggtgagacaa
     2761 attctttttt taagaattcg tctcaccttc tttctgttat ttgctgattc tgttagacta
     2821 gatataatga aaaaagtata gtgaaagaag gaaaaaacat ggaaatatcc gaaattcgaa
     2881 atctcctgac agaaatgcag gaaaaggtca caagcttcag gaggtctctt tgacttagac
     2941 cagctggaag aagacattgc ggaagcagaa gcaaaaatgg ctgaacctgg tttttgggat
     3001 gatagcgaag ctgctcaaaa agtgatcagt gaaaacaatg caaataaaga aacttatgat
     3061 caattccatt ctcttgctga agaactggaa gaaatcgaat tgatggtgga gatgttgcag
     3121 gaagaacctg atgcagccat gcaagaagaa gcagaaacac gtatcaatgc gctagatgaa
     3181 aaaatgaaga catatgaatt atctatgttg ttagatggtc cttatgatcg aaacaacgcc
     3241 atcgttgaat tgcatccagg ggctggcgga acggagtccc aagattgggg cagtatgctt
     3301 ttacgtatgt atacacgctg ggcagagcag catgggtatc aagtagagac actagattat
     3361 caagcaggtg atgaagcagg aatcaagagt gtgaccctac tgatcaaagg acacaacgca
     3421 tttggttatc tgaaatcaga aaaaggtgtt catcgccttg tacgtatctc gccttttgac
     3481 tcagcgaaac gacgtcatac ttctttttgt tcggtagatg tgatgccaga gcttgatgaa
     3541 acgatcgata tagaaatcaa tccagatgat ttgaaaatcg atacgtaccg agcaagtggt
     3601 gcaggtggtc agcacatcaa taaaacagaa tctgctgtac ggatcactca cttgcctaca
     3661 ggaacagtag tagctagtca ggcacaacgt tcacaattga aaaaccgtga gcaagcgatg
     3721 gggatgttaa aagctaagct ttatcaatta gaagtggaaa agaaagaaca agaagcagct
     3781 gcacttcgag gagaacaaaa agaaatcggc tggggttcac agatccgttc ttatgttttc
     3841 catccatatt ctatggtaaa agaccatcgg acaaacgtag agacaggaaa tgtccaagca
     3901 gttatggacg gtgaccttga tttatttatt gacggctatt taaaaatgca tctacaataa
     3961 gaacccattg ttaaattgaa ataaaattgt aagattttgc aagcatgttg aaacattgcc
     4021 atgctataat agctaaagac tgaaaaagat atggagagaa cgccatgatt gaaatgaagg
     4081 atgtaatgaa gaagtactca aatggtacga ccgctatccg caatctctca attgtcatcg
     4141 aacaaggaga gtttgtctat gtggttggtc cgtctggagc cggtaaatct acattcatca
     4201 aattgatgta tcgagaagaa aaagcttcaa aaggaacttt gaacgtcgca ggtcatgatc
     4261 taatagcaat caaaaaccgc gaagttcctt atctacgcag agaaatcggc gtggtgttcc
     4321 aagactataa attattacca agaaaaacag tatatgaaaa tgttgcgtac gctatgcaag
     4381 ttatcggcag aaaacctcgg gatatcaaaa aacgagtaat ggaagtgctg gaccttgttg
     4441 gtttgaaaca caaagttcga gtctttccta gcgaattatc cggtggggaa cagcaacgtg
     4501 tctctattgc tcgggcaata gtgaatacgc ctaaagtact tatcgcggat gaaccgacag
     4561 gaaatctgga tccagaaaac tcatgggaaa tcatgaaatt attagatcgg atcaatgccc
     4621 aaggaactac tgtcgtgatg gctacacata acagtacgat cgtaaatacg attcggcacc
     4681 gagtaattgc tatcgaaaac ggccgcatta ttcgagatca agcggaaggg gaatatggat
     4741 acgatgatta gaaccttttt ccgccattta ttagaaagta tcaagagttt gaaacgtaac
     4801 ggctggatga cagttgcttc agtcagtgcg gtaacgatca cattgacttt agtcggtgta
     4861 ttcatggcag ttatcatgaa cgcaactaaa ttagctcaag atatagaagg aaacgttgat
     4921 gtctctgtat tcgtagatat cggtacaaaa gacgaagaga tgaaaaagct agaaaaagaa
     4981 ttgaatgact tagatcatgt taaaaaagtt gaattttcaa gcaaagaaca ggaattgaaa
     5041 aaaatccaaa acgaaatggg tgattcctgg aaattgtttg aaggtgacaa taacccgtta
     5101 tatgatgtgt atgtagtaag tgctactgat ccttcttata caaaacaagt agcagaagaa
     5161 gcagcagatt tgaagaatgt ttctcgttct gattacggtg gagcttcctc agatcggatt
     5221 ttccaaatcg ctggtgcagt tcggacatgg ggattagttg cagcggcatt gctgctgttc
     5281 gtagcgatgt ttctcatttc gaacacgatt cggatcacga ttttgtctag acagcgtgaa
     5341 attcaaatca tgcgtttagt cggtgcaaaa aatggatata ttcgctggcc gtttttcctt
     5401 gaaggtggct ggatcggtct gatcggtgcg attcttccga ttttgatcat cacatttggt
     5461 tatgcatggt ttttccaatt gatcaatcca tcattattac gttcccatta ttcattgatt
     5521 catccacaaa atatcgtttg gaaaatcaat cttttaatgg tcggcattgg tgtgatcatt
     5581 ggttcacttg gctcgatcat ttctatgaga cgtttcttga aattctaata ccacaatata
     5641 aataaaatga gaaagttgga cagtttcgtt ccagctttct ttttttattg gctaggagaa
     5701 tagaaggaag cggctttttt ccctttactt taaaaaacaa cttgggtatt cttaagttgg
     5761 tagaagaaga ggtgaagccg tgaaaattat cgttgacaat ttttttacga tccttttgat
     5821 tatgaatatt ttgctttcct ttatcatcgt ttttcgagaa agaaaggaaa ctgctcaaac
     5881 atgggcatgg gtgctcgttt tgatgttcat tccagttgta ggatttattt tatatatttt
     5941 tttaggaaga gggatttcca aagagaaaat ctttgatttg aagatccaag ggaaaatcgg
     6001 taagaacctg gaaattgaag aagacaaaca ggcattgatg cgagggcttt atcctcatcc
     6061 gcctactgga caagtagatg tgaaacaatt gatctatatg ctcacggtat ttgaaagtac
     6121 gctctatacg actaaaaatg aaatcacttt atttacagat ggtcgtgaaa aattcgatgc
     6181 gctgattgaa gatatcaagc aggcagaaga ccatatccac ttccagtatt atatttatcg
     6241 cagtgatgca ttaggtgaag aagttcgaga tgcattgaca gaggcggcaa gacgaggggt
     6301 aaaagtcaga gtccttcttg atgcatgggg ttccacacaa gtttcttcca gcttttttca
     6361 gaatttgaaa aaagcaggcg gagaagtcgc atttttcttt cctctatttg ttccgtatat
     6421 taatcctcgg atcaattatc gcaatcatcg taaaatcgta gttattgatg ggaaaatcgg
     6481 atatacaggt gggttcaatg ttggaaatga atatctagga ttggtaaaga aatttggtta
     6541 ttggcgagac aatcatttgc gtatttacgg agaagcggtg tataccttgc aaaaccgttt
     6601 tttgatggac tggaattctc agcatacaaa agaagtaggc tattctccca aattttttcc
     6661 gtccattcac tctactggtg atatagctgt tcaaatcgtg acaagcggac cagataccga
     6721 acatgaacaa atcaaaatga cttatttgaa aatgatttcg ttagccaaac gggagatatt
     6781 gatccaaacg ccttattaca taccggatgg atcgattcat gaagcactga agttggctct
     6841 tctttcaggc gtaaaagttc atatccaaat acctaacaaa ccagatcatc ttctcgttta
     6901 ctgggcaacg tattctttcg ctgcagagct aatcgagtat ggagcaagga tcgaaacata
     6961 tgagaatgga tttattcatg caaagacgat gattatcgat ggtggcattg tttcagttgg
     7021 ctcagcaaat atcgatgttc gttcatttcg cttggacttt gaggtcaata cactggttta
     7081 tgatgaacga atggcttcac gtgtaagaaa agcttttttt gaagactcga aaatttctac
     7141 gcatctcaca aaagaaatgt atgaaaaccg aggaataatc atcaagatga aagaaggatt
     7201 agcaagattg atttcgccgc tcctttaaaa cataaaaagg acatgagaac catcaacaga
     7261 tggagatctc gtgtcctttt ctaacggggc catttctgag agtacgtcag aaatggagaa
     7321 agcgtctcgc atgagcgaaa ttaactttgg ggtatctctt gttttttggg gaaagcaaga
     7381 aataagctaa tatatgctat taaaagagac acatttcaaa gccagtatag catagaaact
     7441 tttaatgaga tagttttttt tacaattatg agaatttatt ttgttattta atgattttga
     7501 ttcgttttca tcaagtaatg aaaatgataa aataaacata ctttaggatt aaaggagatt
     7561 tctaatgaaa aaacttaaat tattcttacc aataatagga ttagctattc ttttatcagg
     7621 ctgttcaaag tggattgatc gaggagaatc cattacggct gttggctctt ctgctttaca
     7681 gcctttagta gaaacagttg ctgaaactta ccagacagaa aatcctggaa aattcatcaa
     7741 tgtacaaggg ggcggaagcg gaactggttt gtctcaagta cagtcaggag cagtcgacat
     7801 cggaaactct gatttgtttg cggaagaaaa atcggggatc gatgcctcag cattagtcga
     7861 tcatcgagta gcagttgttg ggatcactcc tattgtgaac aaggatattg gcgtaaaaga
     7921 catcagtatg gaaaacctga aaaaactatt tacaggcgag atcaaaaact ggagtgaagt
     7981 cggtggaaaa gatcagccag tagttatatt gaaccgcgct tcaggaagcg gaacccgtag
     8041 cacgtttgaa aaatgggtat tggacggaga aacagctgtt caggcacaag aacaagattc
     8101 aagcggaatg gtgcgccaga tcgttgccga cacacccggt gcaatcagct atgttgcttt
     8161 ttcttatgtt tccgatgatg tagatacgtt gaatgttaac ggtgtagaac cgacagatga
     8221 aaatgtgaca acgaacaaat ggacgatttg ggcatatgag catatgtaca caagagatca
     8281 tccaaaaaaa ctgacaaaag aatttttaga ctatatctta tctgatgaag tacaagataa
     8341 catcgttgga gaactaggtt atatccctgt ttctcatatg aaagtcgaac gtgattggca
     8401 aggaaatatc gttaaagagt aatctttaca caaaatttac aaacttagct aaacttttac
     8461 atgttattaa aaccaaattg atgttcaatt tgtacactga gtgagtatgt taaacctgat
     8521 taagggggag tcatctgtgg aagatgtgaa aaaagtatta ttaacaaaat caaaacgttc
     8581 aaaaatggaa cagcgtggaa aattcatcag ttttttatgt atcgcgctga tcgtgttggt
     8641 tgtattatct attttttatt ttgtagctag taaaggattg gccacctttt ttactgataa
     8701 gatcaatgta tttgattttc tgtttggtac agattggaat ccaagccaaa tcggagcaaa
     8761 tgggaaacca atggtagggg cattgcctat gatcacaggt tcgtttattg tgaccttttt
     8821 atctgctatc gttgccacgc catttgccat tggtgcagca gtattcatga cggagatctc
     8881 tcctaaaaac ggaacgaaga tcttacagcc ggtcatcgag ctgcttgtag gtattccttc
     8941 tgtcgtttat ggttttatcg gtttatcagt tatcgtgcct tttgtcagaa gtatttttgg
     9001 cgggactggg tttggtatct tagctggtac atttgtgttg ttcgttatga tcttgccgac
     9061 tgtcacaacg atgactgttg atgcattaaa ggcagtgcca cggcactatc gggaagcttc
     9121 attagcgctt ggtgccacaa gatggcaaac aatctacaaa gtcgtgctac gagcagccgt
     9181 gcccgggatt ttaacagcag tcgtttttgg tatggctcgt gcgtttggtg aagccttagc
     9241 tatccaaatg gtcatcggta atgcagcatt gatgccaaca ggtcttacta ctcctgcttc
     9301 tacattgacg tccatcttaa caatgggaat cggaaataca atcatgggaa cagttgaaaa
     9361 taatgtgtta tggtcattag cgctgatctt gctattgatg tcattattct ttaatatcgt
     9421 gatccgtatg attgggaaga aaggagagct gaaataatga acgcaaaacg ctcagataaa
     9481 attgctacag gtgttttata tatagtttcg gggatcatcg tattgatttt agcagcttta
     9541 ctactttata tcttagttcg gggaataccg cacatttcat ggtccttctt gaccgaacct
     9601 tccaaagcct accaagaagg cggtggaatc gggatccagc tatttaactc gatttatttg
     9661 cttttgatca cgatgatcat cagttttcct atttctttag gtgcgggtat ctatctttca
     9721 gaatatgctg gaaaaaactg gctaacagat gtgattcgca cgtcgattga aatcttgagc
     9781 tcgttgcctt ctgtcgttgt cggtttattc ggtttcttgg ttttcgttat tcaattccag
     9841 tatggtttct cgattatttc tggtgcattg gctttgactg tattcaactt gcctcttttg
     9901 actagaacag ttgaggagtc tttacaagcg gtccattaca cacaacgaga agcaggattg
     9961 gccttaggtt tgtcacgctg ggaaacagtg ataaaagtgg tggttccaga agcgacacca
    10021 gggattttga ctggtgtgat cctaagttct ggaagaattt ttggtgaagc agctgcattg
    10081 atctatacgg ctggacaaag tgcacctgcg ttagatttta cgaactggaa tccattaagt
    10141 gtttcaagcc cgatcagcat tttccgtcaa gctgaaacat tagctgtcca tatttggaag
    10201 atcaatacag aaggcacgat gcctgatggt gtttctgttt cggctggcgc ttctgccgtg
    10261 ttgattattg ctgttttact gttcaacttc ggcgctcgtg cgattggcaa acgattatac
    10321 cgaaaaatga cttcagccta aggagaagaa gcaaaatgaa aaaatataac tggaatgaaa
    10381 caaatgtcat caccatgcca aaagagaaag aagtagcact atatacggat gatctacatg
    10441 tctggtatgg cgacaatgaa gcaatcaaag gagtggatct ggaatttgaa aaaaataaga
    10501 tcactgcact gattggtcct tccggctgcg gaaaatctac gtatcttcgt tcgttgaatc
    10561 gaatgaatga tgggatcgct aatacgaaaa tcactgggaa aatcatgtat caaggcgtag
    10621 atattaatgc gcctcaagtg gatgtttatg agatgcgcaa acggattggc atggtttttc
    10681 aacgtcctaa tccgttcagc aagtcgattt atgacaatat tacatttgct ttgaaacagc
    10741 acggcgaaaa aaacaaaaag aaattagatg aaatcgttga aacaagcttg aaacaagctg
    10801 ccttgtggga ccaagtaaaa gacagcttgg acaaaagtgc cttggcttta tctggcgggc
    10861 aacagcaacg tttatgtatt gctcgtgcga tcgcgatgaa acctgatatc ttgctgctag
    10921 acgagccggc aagtgctttg gaccctattt cgacaggaac ggttgaagaa acacttgtaa
    10981 acttgaaaga agactatacg atcatcatcg tgacgcataa catgcaacaa gcagctcgta
    11041 tcagcgacta tactgccttt ttctatttag gaaaagtaat ggaatatgac aaaactaaca
    11101 agatatttac cagaccaaaa attcaggcaa ccgaagatta tgtgtccggt cattttggtt
    11161 aagacggagg cctcaacatg acaaaagaga ttatttcatc taaagatctt catctgtatt
    11221 atggaaagaa agaagcacta aaagggatcg atctaagctt caatcaaggg gaaatcactg
    11281 cgatgatcgg tccttctggt tgcggaaaat ccacttatct gcgttctttg aaccggatga
    11341 atgacctgat tccgaatgta acgatcaccg gaagtgtttt atataaagac aaagacatct
    11401 atggtccaaa aacagatgtt gttgaactaa gaaaagaaat cggcatggta ttccagcagc
    11461 caaacccatt tcctttttct atctatgaaa atgtcatcta tggattgaaa ttaaaagggg
    11521 aaaaagataa aaaagtcctt gatcaagtag ttgaagaaag cctgaaggca gcgtctgttt
    11581 gggatgatgt gaaagacaaa ttgcataaaa gtgctttatc tttatccggt gggcagcagc
    11641 agcgtgtatg tatcgcacgt gttttggcag ttaatccgga aatcatctta ttggatgaac
    11701 caacaagtgc gcttgatcct gtttctactg ggaaaatcga aagtatgctg ttagaattaa
    11761 gagagcggta tacgatgatc atcgtgaccc ataatatgtc tcaagcttca cgtatttcag
    11821 ataagacggc attcttttta gatggtcact tgatcgaata caatgatacg aagaagatat
    11881 tcatgaatcc agaaaaacaa gagacagaag attatatttc aggtcgattt ggataaggag
    11941 gaaacggaat gctacgtaca caatttgaag aagaattgct gaatctccat aatcagttct
    12001 acgaaatggg gatgatggtg agcagcgccg tgcacaagtc agttcgtgca tacatcaaac
    12061 acgataaaat cttagcccaa gaagttatcg aaaacgatat aaatatcaat aacatggaaa
    12121 cacgcttgga gaagaaaagt tttgaaatga tcgcattgca acagccagtt acaactgatt
    12181 tgcgtatgat cattacggtc atgaaagcta gctcggattt agaacgtatg gccgatcatg
    12241 cggtgtctgt tgctaaatcc acgattcgcg taaaagggca gacgagaata ccggaaatcg
    12301 aaaaagagat ctccgatatg tccgattatg tgaaaaaaat ggtggacaac gtcctagttg
    12361 cttatgtcaa aacagacgaa aaagacgcac gtacgatcgc caatatggat caaagagtca
    12421 atgactattt caatcgaatc tataatgcaa caatcaaaaa tatgcaggaa aatcctgaga
    12481 cagtcatcag tggaacagac tacttgaatg ttgccagtta tctagaacgg atcggagact
    12541 atgtgacaaa catctgtgaa tggatcgtct acttggctac agggaaaatc actgagttaa
    12601 atacgaacca caatgagatt ttctaaactg aagctgtgat tatgccgttt gtttaggaaa
    12661 agaggttgtg aaatcagcgg ttgtgaaaaa gctgttctgc atcaagtaat aagaacagtc
    12721 aaacaaaaaa tagctcctca tatttttcgt ttggctggac ttattaccgc agatgcaagc
    12781 ttttgaacac cgtttattcg ggaaagggtt tgtgacaaaa catgtcacaa acccttttca
    12841 tcatttcata gaaaggataa agaatgcaaa aaaaaattag catcatcact atatttttta
    12901 tttgtgtgct tacttcatgg gttattcagc atcaagccgc tttatatgaa gtacctgtca
    12961 tcgagataga aaaagtacga ataaaagagg atcatcaatt gattacagga aaactgatga
    13021 acacggatca aaaaggagaa tcgatttatt tatcggttcc ttatcaaaaa aatcaaatag
    13081 aaacagcaaa tataaaagcg agacaaatgt atctagtcca aatgcatgga gatgaatggc
    13141 agctagtagc aaaaaaaaat gatggctggc ttttcttctt cacaagtatc ttcattggcg
    13201 ccatgttgct tattggtgga aaacagggtc tatttgcttt aggaagtctc atcatcaata
    13261 tcgttttact tttacttttg ttgctcatct ttactaaaac agaagggatt tctctattaa
    13321 atatatctat cctgttcgtc gttataggga tgattattag catattggct ttagatggat
    13381 ggaatagaag cagtataatc aaaatttcag ctgcagttac gagtgttttt ttagcatttt
    13441 ttatttgtct tttgacaatg aatcattgga aagaccaagg actgcgctat gaggaactga
    13501 attatctgac acgaccgtat cgttctgttt ttctttctag cgtactgatt ggaacagctg
    13561 gaggaacact cgataccgtt atcacagtca ttgcgacatt agaagaattg actaagcgac
    13621 aaccagaaat aacagcgaaa cagttatggc tttcaggaag gcaagtgggt cgagatgtga
    13681 ttggctcgat gaccaatatc cttttgttcg tctatatcag tgggtcagtt ccatcgcttc
    13741 ttttatattt aggaaatcaa tggtcgttca aagaaacgat agaacagcat ttatcattgg
    13801 aatttttacg cgtcattgct ggcagcttag gaatagtatt atcgatccct atctcggtcc
    13861 tcttttttct gactgtaagg aggctgaaac gatgaatgta cttattatac ttgttgggat
    13921 tttagcaatc agcgtgctac ttattggcgg tacgcaaggt atgtatattc ttttaggctt
    13981 attcatcaat ttagggatct ttttcctgtt gttattcggt tatcatcaga agtggccgat
    14041 actggcattg tcggtcatag gatttttatt gattgctgtg gttatcttat tttttatcaa
    14101 cggttataat ctaaaaatgc gtgcagcttt tgtgagtata ctgatctttt tattttgttt
    14161 cctactattg cttcctatca ctgatttttt agcaatccaa ggttttacat cggtagaact
    14221 agaagaatta tctggcttag ataagacttt agcgattgat tttagattac ttacgagatc
    14281 gttgcttctg attagtctta gtggtgctgt tttagatgcg agtgtagcaa tttctagtgg
    14341 gacattcgaa gtatatcagg caaatccgca attatctttt taccaattgc gccattctag
    14401 tttcgctatt gctaaaaaaa tattggcgtc aacagtaatg acacttcttt ttgcattcat
    14461 gggaagttct ttagcgttga tcctctggtt tattgattta gacattcctt ttcagcaagt
    14521 gatcaatgaa aaatcatttg ttctagaata tacgatggct attcttacca cattgtccgc
    14581 actacttgtt cttcctttaa cctgcgtgac tacagcatat ctctttacaa aaaagaaaga
    14641 acaactcaag atggagtaaa agttacaact taagtcctat gacattttta ctgaatcagt
    14701 gcataatatg gatgtaagga aaagcttgca aacaattttg cttaaggagg caatttccat
    14761 gaaagaaaga gaacgtattt tagatttagt gaaaaaagga atcctttcga ctgaagaagg
    14821 cttagattta ttagaaagca tggcaactga aaaagacgaa aagcaaatca aaaaagaagc
    14881 agacaaggta actgctagtc ataaagaaaa agatcaggca agtcaattga tcgataaact
    14941 tgaaaacggc gaagaagaaa tttctgagcc agttgatcca aaggaaagag aaagacaaga
    15001 tcaagaaaac ttagaaaaga tcttagatga actagcaact gaagcgaata aaacttcggc
    15061 gcgtctagat gaagtaaatg ctcaattcgc tgacaataag gcagcacgca atgagaaaca
    15121 ggaagcattg atgcagctga atacaaaaga agaattaggc gagttgacag aagaagaatt
    15181 agcacagcgt cagacgttag aagcagaaat caaagaatta gaagctagtg gcgatacact
    15241 gttagaagaa caattgaaat tagaagcaga attaaaagat atccgtaaga atcaatggtc
    15301 tgagaaaaaa gaaacgttta ctgataagtt tgaattgcca gatgattgga aagatcaagc
    15361 aacagataca ctgaatcaag taggcgaaaa aatgtcagaa gctggatcac agttaggcaa
    15421 attcttgaag aaaacattcc aaactgtttc agaaacagtg aatgacaaca tggaatggaa
    15481 agatgtcagc ttacgtgtcc ctgggattgc gacaacgaaa tttgagcatg aattttacta
    15541 tgaagctcct gcagcaagta tccttgatat caaggcagca aacggaaacg tgacgttaaa
    15601 aacgtgggac agcaacgatg tgaaagttga agctaagatc aaactttacg gaaaaatggg
    15661 tgctgaacca tttgaagcat tctctgaacg gagccagatt gaggttaatg aagatcatat
    15721 ctccttccaa attccaaata aacgtgtgcg tgcagacctt gttttctact tacctaaacg
    15781 agtatacgac cacgcagcta ttaaacttct aaatggcaac atcatgattg aaacattgga
    15841 agctaaagat atttatacaa aatctacgaa tggtaacatc atcgtggacc aattaactgc
    15901 aacgatgctg gaagtagaag gtgtaaacgg caatatcgat attcgaaacg ggaacatctt
    15961 agattctatt atcgaaacag taaatggtac agtgacattt ggtgcgacag cagaaaacct
    16021 ttctgtgtct ttggtaaatg gtgatgtacg tcttacgatt aaagaagata atttgaaaaa
    16081 agtagaagca agttcagtga acggaaatgt caaagtcgct ttaccagatg gcatcggttt
    16141 ggaaggacat gctaaaacaa gtcttggaag tatcaacagc cgtctgtcca attacgaagt
    16201 agttcgtgag aaaaaagaac ggacaaacca aatgttgcaa ttccgtcgtg tttcagatgg
    16261 tgaaatcgcg caagtgcaac tttcaacgac aacaggaagt atctatttaa aagatacaga
    16321 taaataaaga ggttgtgaaa gagatgttca gccttgagca ataagaagcg attttgtaaa
    16381 atagcttccc ctattttaca aaattgtgcc ttattgccga tagtctatct cttgaacacc
    16441 gtttaccaag agattatgaa cctgccaatt atcgccgagt cacaaggaat aatttcagaa
    16501 aaagctcgtc acgttttatg aatatggtaa tttgtgaccg agaattttgc agaaagacac
    16561 cgattaagaa gaaggttgtc acaacgattt tcttcattta aaatagtaga acagaggtga
    16621 gacatgatga aaaaaaagtt gacaaaatct aataaaaatg ttgttttgac agggacttta
    16681 gcaggaatag gagaatacat cgggattgat ccgactgtta tccgtgtgct ttatgtattc
    16741 ctgagcttag ttgcttttgg aagtccaatt attttataca ttttattagc tttgattatc
    16801 ccatctgcgc gttctgcacg aacaaactat ggacataaca atgcatatta tgaaaaaaac
    16861 aattatcgtg aacaaactcg tgaacaaaca aaaaaacgca aagaagcgga aaaaatcgat
    16921 gatgacgaat ggagtgactt ttaatgtcgt actttcaacg tattttagtt aatacgttaa
    16981 cattcatatc attggctgtt ctttttcctg cgatggtcca tgtgagcagt atttggacgg
    17041 ccgttcttgc cagcttcgta ttgtctgtgt tgaatatgtt tgtcaaaccg attttgacga
    17101 tactgtccct gccctttaca ttagtcacat tgggattgtt tacgtttgtt atcaatgcag
    17161 cgattcttaa aatgacctcc ttttttgtgg gagcagaaca ttttagtttt tcttcattcg
    17221 gtgcagcgtt gttagtagct atcttgatgt cgcttgtcaa tgtaattgtc agcgaacata
    17281 acagtagaaa ataaaagaaa gctgtgccga actatcggcg cagttttttt atttagaatc
    17341 aggaatgcgt tttctattac attggtaatt aaataaaaac gtttttttta attggtagta
    17401 cttttggcta gacaatgata aaatgataga gaacactcta atagaaatga ggtagcatga
    17461 atgtcagaag ttgtaaaagt tagccaatta gtgaaaaaat tatctttgga aatcgtgaca
    17521 ggcgacgaga aaagcttaga ccgtgtcatc gtaactgggg acatctcacg cccaggacta
    17581 gagttgactg gttattttaa ttattattcc cataatcgtt tacagctatt tggcagtaaa
    17641 gaaatcagtt ttgcggaacg gatgatccca gaagagcgtt tgatcatcat gagaagatta
    17701 tgtagtgaag atatgcccgc atttattatt tcaagaggat taccagctcc agaagaaatg
    17761 atccaggcag caaatgaaaa tgggatagct gtactgcgtt ctcccatctc aacttctcgt
    17821 ttattaggag aattatccag ttatttagat ggtcgccttg cccctcgaac aagtgtccac
    17881 ggtgttttag tggatgttta cggattaggt gtactgattc aaggtgacag tgggatagga
    17941 aaaagtgaaa cagcgttgga gttgatcaaa cgcggccatc gattgatcgc agacgatcga
    18001 gtagatgttt atcagcaaga tgaattaacg gtgatcggag aaccgcctaa aatcctggaa
    18061 catctgattg aaatccgagg aatcgggatc attgatgtaa tgaatctttt tggtgcgagt
    18121 gcggtacgtg gctctatgca agtccagttg gctgtttatc tggaagcatg ggcaaaagat
    18181 aagaaatacg accgtctggg ctctgaagac acgaccgttg aaatcgcgga agtgggcatt
    18241 ccacaaataa aaatccctgt caaaacagga cgaaacgttg caatcatcat tgaagtagcc
    18301 gcgatgaatt tccgcgcacg tacaatgggc tttgacgcaa cgaaaacatt tgaagaaaga
    18361 ttaagtcgct tgattgaaga aaactcaggc aacgactagc aaggagtgac aatatgttag
    18421 cacaaatcaa tcgagttgct tttgagcttg tagggattcc tatctactgg tatgcattga
    18481 tcatcgtatc aggtatcatt atcgctatgt ggcttagcag cagagaggct gttcgggtag
    18541 gattaaaagc agatgatgtg acagatttta tgttgtgggg attgcctatc tccattattg
    18601 gcgctcgctt atattatgtt ttatttgatc ttccccagta tatagctgat ccaatccaaa
    18661 tattcaatat ccgttcagga ggcttagcta tttatggcgg actgattgca ggcgcgatca
    18721 cgctgtattt ttttactaaa tatcatttta tttctttgtg gactttttta gatatcgctg
    18781 caccaagtgt attattagca caggcaatcg gtcgttgggg aaatttcatg aaccacgaag
    18841 catatggacc agacacgacg cgaagcttct tagaaagtct gcatttaccg caattcatca
    18901 ttgataatat gtatatcgaa gggatctatc gtcaaccaac atttctttat gaatcacttt
    18961 ggagtttgat tggatttatt ttgcttttgt ttttacgaaa gaaaccacac cttttgaaaa
    19021 aaggcgaagt ccttttatgt tatctaatgt ggtatagttt cgggcgtttc tttattgaag
    19081 gattacgtac agacagtctt tactttttag gtgtgatccg tatttctcaa ttattatctg
    19141 ctgtgttatt tgtcggtaca ttgattctat ttatttggcg cagaaaaaaa ggaaatttac
    19201 cggattatga ccgctcatta ggaaaaaatc aacaaattat ctgattttta gagcgttaaa
    19261 aatgaaaaaa atttcaggag tggtaaaatg aattatagag tagcggtttt aggaccaggc
    19321 tcttggggta ctgctttggc acaagtcctc gccgaaaacg gacatgacgt tcggatttgg
    19381 ggacatcgtg cttcacaagt agaagaaatc aacacccaac acacgaatca acgttattta
    19441 ccagatcatt atctacccga ttccatccaa gcattcacca atatggaaga tgcagtaaaa
    19501 gacgcagatg cggtgctatt cgtcattcca acgaaagcaa tacgttctgt tgcaaaagaa
    19561 ctagtgccta aattgaatac aaagccagtg atcatccatg caagtaaagg attagaacag
    19621 ggaacacata aaaggatctc tgacatcttg ttagaggaaa tcccagcaga aaaaagaaaa
    19681 gctgttgttg ttctttctgg accaagccat gcagaagaag tagcaaaaca tgacatcact
    19741 acgatcacag ctgcaagtac agatgcaaaa gcagcaaaat atgtacaaga gctgttcatg
    19801 aataattatt ttcggatcta taccaatcct gatgtgatcg gtgtagagat gggggcagct
    19861 ttgaaaaata ttattgctat tggtgctggc gctattcacg gcttagggtt tggcgataat
    19921 gcaaaggcag caatcatgac gcgcggatta gctgagatca gccgtttagg tgtagcaatg
    19981 ggtgccaacc cgctgacctt tatcggatta agcggaatag gcgatctagt cgttacttgc
    20041 acaagtgttc attctaggaa ctggcgcgca ggcaacttgt taggacaagg gcagccccta
    20101 gaagaggtgc tagccaatat gggaatggtc gttgaaggtg tgaatactac ccaagcagcg
    20161 atggaactag cccaaatgat ggaaatcgaa atgcccatca ctcaaacaat ctatgaagtg
    20221 ctgtatgaac aaaaagaaat caaaacagcc gcaaaagaaa tcatgttacg tgatggaaaa
    20281 aaagaaaatg aattctcatt aacaattttt tgaaggagcg aatgtatatg aaagttaaaa
    20341 aagcagttat tccagcagca ggattaggaa caagattttt accagcaaca aaagctatgg
    20401 caaaggaaat gttgccaatt gttgataaac caacaatcca gttcatcgtt gaagaagcgt
    20461 tgaaatcagg tatcgaagac atcttgatcg ttacaggaaa agctaaacga ccaatcgaag
    20521 atcactttga ttcaaatatt gaattagaaa caaacctaag tgaaaaagga aagaccgaat
    20581 tattgaaatt agtagaagaa acgacagatg tcaacttgca tttcatccgt caatctcatc
    20641 caaagggttt aggacatgcc gttcttcaag caagagcctt tattggaaat gaaccatttg
    20701 tcgttatgct aggcgatgat ataatggaag acgaagtacc attgacacag caattaatcg
    20761 atgattacga acgtactcat gcttctacga tcgccgttat gcaagttcct catgatgaaa
    20821 cttcaaaata cggaattatc aatccaggag aagtattgga agatgggtta tacaatgtca
    20881 aaaactttgt tgaaaaacca gatccaagca aagcaccaag tgatttagct attattggac
    20941 gttacttatt gactccagaa atttttgatg tattgtccga gcaacaacca ggagccggaa
    21001 atgaaatcca actaactgac gcaatcgata cattgaataa aacacaacgc gtatttgccc
    21061 gtaaattcac aggtaaacgt tacgatgtcg gtgacaaatt tggttttatg aaaacaagta
    21121 ttgaatatgg tctaacacat ccagaagtag gtgcaccttt acgtgagtac atcctgtctt
    21181 taggtaaaac attagaagca gaaggaagca agatcgatca taatccaaaa ggaataacag
    21241 aaatcaaaga agaaaagaaa aaataaccct tttttctaac gagcttcagc tgctaattga
    21301 ataaaaagta gttgaagctc gtttttattt gtttaaaaaa cacgaaaatg ctatccttat
    21361 agaaaagaga gacaagaagg gagtttgatc atgtcaggag gagaaattgc aggattgatc
    21421 gcagcaatcg cctttgcgat acttgtcgtt tttatcgttc gtgtgttgtt gcaagtttct
    21481 aaaactttag aaaaagtaga tcagactgta gcagaagcaa atacgacgat cgaaatcgtc
    21541 acaaaagacg tagatctttt gtctagacaa gtagaaggct tactggtcaa aagtaatgaa
    21601 ctgctcttgg atatcaatgg aaaagtcgca acgatcgatc cattgttcac agcagttgct
    21661 gatttaagtg aaagtgtatc agaacttaat cattctagta aaaacatcgc gacgaaagtc
    21721 ggcggaatcg gtaaaacgac agctaaagcg acggttgccg gaaaagtcgg tgggacagcg
    21781 atgaaatttt ttaaaaacaa aaaacatact gaaacgacag gaggaaaata aaaatggcta
    21841 aaaaaggtgg atttttatta ggtgcagtag tcggaggcac agccgcagca atcgccgcgt
    21901 tattgttagc accaaaatct ggtaaagaac ttcgggatga attagctgcc caagcggatg
    21961 atttgatgga tatggcttca gattacacag atatggcgaa agaaaaatcg actgagttaa
    22021 caggtattgc aaaagaaaaa gcgcaagatc tttctcaaca agcaggtgat ctagcaggta
    22081 gtgtcaaaca aaaagtaagt gaatcagcag atgaagcagg aagtgaatct gacgatatct
    22141 tgttaaatct taaagaacaa tcagctgact tatctaatcg tttcaaaaaa acggcagaag
    22201 atatgaaaaa acctgcagat gaattaggaa gtattgctaa agatacttca gaagatatca
    22261 tgattgatgt aaaagacgct gctggtaaag caggagatac tgtcaatgaa ggaatgtcag
    22321 aagcaaaacc agttgcagat gactcaaaag aaatgatgaa aatggcaaaa gaagatgcga
    22381 aagaagctaa aaaagaaata aaagatacaa tgacagattc atctacaaat cactaaaatc
    22441 acaaatttat agaataaaaa ggtgggagcg gactagctct cacctttttt actcttattt
    22501 ttctaaaaca agtaattctt ttggtgtagt cgtgaatggc acacagccat cgcttgtcac
    22561 atatacacag tcttcgatac gtacccctac tttttcagga atatagattc ctggttcaag
    22621 agagaagcac atcccttctt cgattactaa gtcatttcct tctacaaggg aagggaattc
    22681 gtggacggtt gttccgatcc cgtgacctaa acggtgattg aaatattcgc cgtagccata
    22741 gctgttgatc acgtttcgtg cgatttgatc aagttcacct gctgtcacac ctggacggac
    22801 ggcttcttgt gcggcaagct gtgcttcaag agtgatgttg tagatttttt cttggaagtc
    22861 ggttggtttt tgataagcaa ttgtacgagt agcatcactg caatagccat tccaaacaac
    22921 gccaagatcg aataacacaa gttcattcgg tgaaatcttc gtatttcctg gtgtaccatg
    22981 agggcttgca gcatttgctc ctgcaaggac taatgtgtcg aaagacattt gtgaaacacc
    23041 ttgttttttc aattggtatt caatttctgc aatgatcgct tgttcagtca cgccttcttt
    23101 cactgcttta aagccaatct caaatgcaac atcggcccat ttaccagctt cgatcagttt
    23161 gtctttttcc tctagagttt tgatcagttg tagacgttgg atgatggaag ttagatcagt
    23221 gtaagaatcc gttcccgtca agatatcaga gatagcatcg aagcgctcga cagtcaaggc
    23281 gttcttttca atccctacat tgccaggctg accatagcga gcacgaagtt cttttccgat
    23341 gatctcccaa gggttttcgc tgtctaaata gccacggaca tcatacgccc aactactgtt
    23401 ttgagcatct tcaacttcca aagcaggagt gaaaaggaaa ggatcttgtt caagagggat
    23461 aaaaagagct agtacacgct catgaggatc actttcgtat ccggaaaagt aagcgatgtg
    23521 gccagggtcg ctgatataag ccaattcgac ttggtgttgt gccatccatt gacgtagttc
    23581 attgattttt acgttattca tttggcaacc tactttctaa attaattttg tattcaataa
    23641 ttgtaccact gatttattaa aagaccaacg ttctaaaaac tgaaaaaagt aaaaattttc
    23701 atgaaaacag agataaaacg gttgcaattc ttgagattat ttgatattct attttagaaa
    23761 tgtaagacgt attttcagaa aacatataaa atatgaatag gagaacacaa atggaaaagc
    23821 aaacaatcac tatctatgat gtagcgagag aggcaaatgt ttcgatggct actgtttcac
    23881 gtgtcgtaaa tggcaatcca aacgttaaac cagcaacaag aaaaaaagtc ttggaagtta
    23941 tcgatcgctt ggattatcgt ccaaacgcag ttgcgcgtgg cttggcaagt aagaaaacaa
    24001 caacagttgg tgtaatcatc cctgatgtca gcaatatgtt ctttgcttct ttagcacgtg
    24061 gtatcgatga tgtggcaaca atgtacaaat acaatattat cttagcaaat tcagatggca
    24121 acgaccaaaa agaagtcaat gtattgaata acttactggc taagcaagtg gacggcgtga
    24181 tcttcatggg tcaccatatc acagacgaaa tccgtggaga attttcacgt tcaaaaactc
    24241 ctgttgtatt agctggctct attgatccag atgaacaagt aggtagcgta aacattgatt
    24301 atgtatccgc aacaaaagat gcagtaaaca aattggcaaa aagcggcaat aaaaaaatcg
    24361 cttttgtcag tggtgcgttg atcgatccca tcaatggaca aaatcgtctg aaaggctata
    24421 aagaagcatt aagcgaaaat aacctaccat atagcgaagg attgatcttt gaagcacaat
    24481 acaacttcaa agatggtcta tctctagtcg atcggatcca taatagcggt gcaacagctg
    24541 tctatgtttc agatgatgaa ttagccatcg gtattttgga tggtttatta gaccgtggcg
    24601 tgaaagtacc ggaagaattc gaaatcatca caagtaacaa ttcactactg acagaagtag
    24661 ctcgtcctcg tttaacaagt atcacacaac cattatacga tttaggggcg gtatctatgc
    24721 gcctattgac gaaaatgatg aataaagaag aagtcgaaga aaaaacgatt atcttgcctt
    24781 atggaatcga agaaaaagga tctacaaaat aataaaagga caaccattca gaggttgtgt
    24841 gcctgccgtt taactctgcg ttacaaggag caatttcaaa aaacagcatc tcatgtttga
    24901 tgaatattgt gactcgtgat tcggagttgg caggagaaca ccgtttattt gggaaaaggg
    24961 gttatgacaa aattttgtta taatcctttt tttgtgtctt aaaaaagaga gaaagtggtt
    25021 gaaattacat ttatcgtata taaaacaatc cgtgtataga tgctgcattt tgttcaccac
    25081 catcaaaaaa ataagggact gtgacaatac tattgtcaca atcccttatt actgtgtatt
    25141 ctattaaatc aatcatcttt ctcatctgat tctttagttg cttgattcgc tcttgcataa
    25201 gctgaagcgg tgttccagta atctgtatag ttttcatcgg taccgccaac gccaaattta
    25261 aaggcgcttt tcttcggtcc atttttcgcc catagcgatt caactgtttt tcctggtgtt
    25321 tggatggatt ttttattgac agtaactttt ccaggtgttt gaccagtaaa tccagatact
    25381 tttttcttca tcacatcaga tgataattcg aatttctcat cagtgccaaa aatgtcaggt
    25441 tttgcttggt aagcttgatt gatcagattg gcaagataat tagccgaacg tatccttgct
    25501 ttgctgtcca ttccagtatt gtcatcgtgc cctgcccagc tactgatggt aatagacgga
    25561 gtagagacaa ttagccatga atctcggtat tcattggtag aaccagtttt tccgacccaa
    25621 tctgcttttc ctagatttcc gtttaagctt gagatggcat ctttgaatgg tgtagtgatt
    25681 tttgcattga tcacactacg catcatgtca ttcatgatcg atgccgttgc ttccgaatag
    25741 atccggacag agttgctttc gtgtttataa attacatttc ctgcgttatc tgtgatggaa
    25801 tcgatgatat agccttcttc atagacgccg ccgtttgcta aagcctgaaa tccgttggtc
    25861 tgttgaagcg tcgtgacatc tactgtacct aaaggagcag attcaacgcc ccaattgtca
    25921 tttgccgggt agttcatctt tgccaagtag tgctcgtaag catattgttt cgatccaccg
    25981 gaattcaaca catcttgata taagtgataa gccgggatat tgttggacca ttctagtgac
    26041 tcacggacgg tttggaaggt gttcgatcct tcgttagtag cattgacgat tttctcgcca
    26101 gcattttctc cttcttgcca agttgttgca taatctgata cacgggattc tgatccgata
    26161 agccccatgt cgattgcagg tccataaaca agaactggtt tgattgatga gccagcttga
    26221 cgttctgtat cgaaggcatg gttattttga ttctgactgt aattccgtcc gccaacgaat
    26281 ccgtagatgc ggccagttct gttatccatc aatacatttc cggtttctac ttgactgcca
    26341 gcaccatcat ctaacatgta tccataattt tcaacgccat tttgcaatgc agcatagaca
    26401 tctttatcga ttgtcgaatg gattgtatat cctttatttt ggatcgtttg ctgtgccttt
    26461 tgataataag cgtcactgac cgatgaatca gacatatcag cgttgtcttt ttcagccaat
    26521 tgttgtgcaa tgattcttgt tgcttcattc atcacagtat aatacaagaa ttctctgtca
    26581 gtctgttctg cgatttgttg tggtagaaag tcttttgtaa ggtcataggc ttttgcttct
    26641 tcgtattgtt ctttcgtaat ttggccttca cgatacatac tgaataaaac aaaatctttt
    26701 ctggcaaggc cggcagaaag atcttctttt agcgcgcctg tgttcgtgta tggactatac
    26761 gtgatcgggc tttggggtaa tccagcgata taagcagctt gcggcagcgt cacatctttt
    26821 gcatctacac caaagattcc ttttgcagct tcctggactc cagcaatgtt ttgtccctta
    26881 ttattacgac caaatggtga gacattcaaa tacgtagcaa tgatctcatc ttttgaaaag
    26941 tatttttcta cttgtgcaga aagaatgatc tcgtttgctt tacgtttgaa tgttgtttca
    27001 tctgtaagga tctgctgttt gactaattgc tgtgtcaaag ttgatccacc agaagagcca
    27061 ataccagtag cttcagatac taatgctcgt aaaacagctt taggaacgta accttggtgt
    27121 ttatcgaagt attcatcctc tgtgctgata attgcagttt tcaataaagg tgaaatctta
    27181 tcagatgaaa tagtcgtgcg catcagatct gtctggattt ttgatatctc tgtattatct
    27241 gcataaacaa tctttgacgt ttctgtaatg tttcctagtt ctgtctgcag tgctttttta
    27301 gtcggcaatt gtgtgtcttc gacgagatag gcaaaatagc cagcgcctat tccaatacct
    27361 agagctgcga acatcaaaag aagaacagta ataaaaacag tcaatgattg aaaaactcta
    27421 aaaataatct taggaacaaa ccatttttct ttttttcttc tattctgttt ttttcgagta
    27481 tttttgcgtg gcaaaagggt cacctcaact aatggtttta tttcgctaat tatagcaaat
    27541 aatagaacaa aaaaacagac aaggctgttt ttggcaggaa tttctttttt aaaagagtta
    27601 aaacgtgaga aaatgatctt tattataaaa gagttcttaa gcttttcttt agagaaaatt
    27661 tcagttacta tggagaacag ggcaaagaaa aatcaatcat tttctttgta tatcatctta
    27721 ccaatatata ggaattgtta gtaacaattg cctttcaagg aggaatccat ccatgttatt
    27781 aggctcacac gtcagcatga gcggaaagaa aatgctgtta ggagcagcag aagaagcagc
    27841 cagttatgga tcttcgactt ttatgatcta tacaggcgcc cctcaaaaca ctagaagaaa
    27901 agccatcgaa gagatgaata ttgatgccgg aaaagcgtac atggcagaaa aaggtctaac
    27961 aaatatcgtc gttcatgccc cttatatcat taatttgggg aatacgatca aaccagaaat
    28021 gtttcctttt gccgtccaat ttctgagaga agagattgtt cgtgcggaag ctttaggcgc
    28081 tactcaaatt acacttcacc caggtgctca tgtaggggca ggaacagaag ccggaattgc
    28141 tagaattatt gaaggcttga atgaagtttt gacaaaagaa cagacggcac aaatcgcttt
    28201 ggagacaatg gcaggtaaag gtacggaaat cggtcgaaca tttgaggaat tagcgcaaat
    28261 catcgatgga gtaaaattga atgaaaaatt atccgttact tttgacacct gtcatacgaa
    28321 tgatgccggt tataatatta aagaagactt ttcaggtgta atggaagagt ttgataagat
    28381 catcgggtta gatcggctta aagtactcca tatcaatgat tctaaaaatc cgcaaggttc
    28441 gcataaagac cggcacgcca atattggttt tggaacaatc ggattcgaca cgctgaataa
    28501 gatcgtccat gatccaatgt ttgacaatat ttcgaaaatc ttggaaactc cctatgtagg
    28561 caaagataaa aagaaagctt acgcaccata tggaaaagaa atcgccatgt tcaaagcgca
    28621 aacattcgat ccagaagtgt ttcctgagtt attagcagaa gaagtgatac tctaagattt
    28681 tctatctgac agcagcaaaa aaggaccagg cacattgcct tgtccttttt tgtttaagaa
    28741 acaaaggagt actttttgca gtctttttca tcatgcatct tctcccccct ctttttaaat
    28801 ttagacaaac aaggtatact ggtagaaaag taaaaaaacg aaaagtttca caagcaagtg
    28861 gcaaaacgtg attggaagga cgaaaagatg ctgaacactt taaaaccctt gatcaaccaa
    28921 ctaaaaaaga aacatacata ctttattctt ggcatagccc tattatttac aattattcgt
    28981 ttgtatttat atagtaaagc aatctatgga acagacatgt cggcaaccta tgatgaccag
    29041 ctgctgattc gctatgctca aaatattttg gacggtaaat ggctagggga gtatacgact
    29101 acaacacttt ccaaaggaat ctcctacagc ttatttttag tacttgcaca taaactgcat
    29161 ctttcttatg cagttttgtt cggcatgttc agtatttttg ccagtatatt ggttgcactc
    29221 gcgtttaaac cgattgtgaa gaacaagtat cttcttttaa gtatctattt ttattttttg
    29281 ttttctccga ttagttttac acatgaatat tccactcgaa catatcgtaa tgccctcgtg
    29341 attccggctg ttgagatcat tatcgcttgt ttattagcta tttattaccg caaatacggt
    29401 tctagcagaa aattgctgcc atggttttta gggctttctg tcgcttttcc tttcttttgg
    29461 ttcattcgcg aagattctat ctggcttctt ccttttactg tagtagcttt gggtatcagt
    29521 ttcgtccaga tcttgctagg gcaagacttt tctttcaaag aagtaaagaa gttaaaaaaa
    29581 cgactctttc ttctctcgaa acgggaatgg ctgaaacttt cattgttttt acttcctata
    29641 gttggctttt ccatcgtcta tcaatggatc gaatataaaa atgagacaac atacggtatt
    29701 gcaaccatca atgaccgttc cagtggagaa tttggacgtt tgatgaaaca actgatccga
    29761 atagatgatg aaacgaactt gaataaagaa aacagtgaga tatgggtctc acatgctgca
    29821 ctaaataaag cattagccgc atctcctaca ttccatactg tttctacccg tattgacgaa
    29881 ttatatacga ctcacgcgtg gactcaaggt ggaaaagtaa aagaattacc cggagatatc
    29941 atcttttggg cattgcgaga tgtggtaagc gaatctggtt attaccaaaa caatgcaaaa
    30001 gaaaccaatg atttttggaa aaaagtcaac caagaattag aagaagccta tcacaaggga
    30061 acaatcaaaa agaaaaaaga gttctatctg atgggatcag gagatggaaa gacgatgagt
    30121 gatctgccaa tcgtattcga tttccttcaa tcaggatacg cttatgcatg gcgatataaa
    30181 gatttccatc aaggaggaaa ttatagtttc ggaacgcaag aacaagtcaa acaggctcaa
    30241 gaattattaa accttccgct aatggagaat tggatcgata gtaatgaacc gagaacacct
    30301 aatttccaat taacaaaatc agcaaaagtc gcgaatgtca tcataaaaat ttaccaagtt
    30361 ctggctattc ccgtgctgat tgcttcaata gcagggatat tacttatgat actaggaacc
    30421 gtatttgcaa aagctgatcg tgcctattat agttcctctt tgattgtggt caccggatta
    30481 atactgacac aattggtttt tcttttcggt gtctcatggt tttgttctta tgcgccagaa
    30541 aaaaaggact attttttaag tgtctacact ggtgcaggcg tgccattaac gcaattcatc
    30601 gtagtaacaa gcatgctagt tttcagtaaa ttaaatctga aaaaagcttt atcttctaaa
    30661 taaaaagacc cttggtagct agatttttaa cctaactatc aagggtcttt gtgtggaaaa
    30721 atcagttttt attcgatttc ttctaccgtt tcctgcaaaa gctgttcata ttttttgttt
    30781 cctttatata aatcaccaat cgtccatgcc aacaggagaa ggacaagaac aatgattaca
    30841 taagcgatgt gatcagccaa tgctagttga gcggaagtgg cttgctcgcc gaagaaactt
    30901 tcgatctgtc ctggtaatcc tcgtaagttc aagtaagtta gactaatcac agagaaccag
    30961 ccaagaagtt ttacgatgaa tgaattttta aatcggctac ccatctccgc ttggctgttg
    31021 gtcatcatca gtaaaggaat catcgaaaac ggcaacgcaa aagccaagaa cacctgggat
    31081 tcattcatta agttattcaa agcggcatgt tcttcgatcg ttccttttgt acttgtaaac
    31141 aaaacacaaa tcaatacagg aatcacagaa agcaatcgtg tgaccagtct tcttagccaa
    31201 atcgggatac gcatatggat gaatccttcc atgatgactt gaccggtcaa cgtaccagta
    31261 atagtagagt tctggccaga agcaagaagt gctacagcaa ataaagtgga caagatacca
    31321 gattttgcta cagcaatcaa aataccattg ctcatagctg atggatcaga taaagcttca
    31381 tacagaccaa aaaaggaagg atcttggaca gaaccggttt tgaagacagc gacacccatg
    31441 atcaatagca gtgcattgac tagaaaagcc attgacaact ggatattaga atcccaagta
    31501 gaaaaacgta cagcttgtgc tacatcttct tcgttttggt gatcgatctt ccgagtttgt
    31561 gaaactgctg aatgaagata taagttatgc ggcatgactg ttgcaccaat aatccctaaa
    31621 gctccagtca gcggagtttc gccgccgatg accggtgagt ctgagaatgt atgactgtta
    31681 gggataaagc cagcaaataa actgtgccaa tctgggttag acaagccaac ttgatagaca
    31741 aaaacgaata aaatgacgaa aattaagcag acaaccagtg cttcgatctt acgaaacccg
    31801 acttttgtca gtagtaagag aacgaaaaca tctaatacag taataaagac ggcaatcacc
    31861 aatggaatat caaaaagtaa gtagagggcg attgctgcac caatgacttc tgcaatatct
    31921 gttgccataa tggctaattc agtcaagatc cataaaatga cacctaatgt cttacttgtg
    31981 cgtgctcgga ttgcctgtgc caaatccatt tggctcacga ttcctaattt tgctgccata
    32041 tactgtaaaa gcatagcaat cagactagac atcaaaataa ctgacatcaa taaatattgg
    32101 aaattttgtc ctccggtgat cgaagtagac cagttacccg ggtccatata cccaactgct
    32161 actaacgcac caggtccaga gtaagcaaga agtgttttaa aaaaactttt cccctttggt
    32221 acatcaactg ttccatttac ttcagctaaa gataaaccat ttgtatgttc aataagtcga
    32281 tgcttatgaa attgtttatt ggattgatta ttcaagaaaa aaccaccttt cctatttatc
    32341 tacgattaca ttctacacct caaaaagaaa aaattaaaga aaaagattcg gtacgcctaa
    32401 ttttttttca tggcgcgtct tgctagatga cgaaagaaat tttgttctcc tctaaaaaaa
    32461 ccactatttt ttgcatgaaa ctaaagaata cgcctttctt ttttcaataa actttagtaa
    32521 aataagtcat tgagagcaat tcggaaatag aaaggagaga tttatatgtt atcaacaggt
    32581 attgtcgtac ttattgctat tattgctttg ctaatcggcg cagtaggtgg ctttttccta
    32641 gcgcgtaaat atatgaaaga ctatttagaa aaaaatcctc ccgttaatga ggacatgttg
    32701 cgatcaatga tgatgtcaat ggggcaaaaa ccatctgaga agaaaattcg ccagatgatg
    32761 cagcaaatga agaaccaagg gaaaaaatag gttctgaccc aacgactttt tacgggctga
    32821 ttttttcagt ccgtttttta tttcgtaatt tgttagacta ttcagattat tcagcaaaaa
    32881 agacaagcac agacaatcct aggctcttga taaagacaaa ggaggagtaa aatgtcgatt
    32941 tttagaaaat taggctggtt ttttcgtcag gaaaagaaac attatttaat tggggttttc
    33001 tcgctattcg cagtggcctt ggtacagtta gttcctccaa aagtgattgg gatcatcatt
    33061 gatgagatcg ccgataaaaa tttatcaata aagatcattt tcgggtggat cttcctattg
    33121 attatcgctg cttttttaca atatcttttt cgttatatct ggcggacgaa tatttgggga
    33181 agtgcagctc gattggagaa agaacttcgg cagcagttat tcaaacattt tacagaaatg
    33241 gacaatctct tttaccaaaa atatcggact ggggatttga tggctcacgc aacgaatgat
    33301 ttgaatgcga tccagaatgt agcaggagct ggtatcttga cctttgcaga ttcagtgatt
    33361 acgggcggaa caacgatcat tgcgatgatt ttgtttattg attggcgctt aacactgatt
    33421 gcgctgattc ctctgccttt gctggccgtc acttcacgag tgctggggtc aaaactccat
    33481 gatgcttttc gtgattcgca agaagctttt tctagtatca atgataagac acaagaaagt
    33541 atcactggga tcaaagtcat taaaacattt ggacaagaaa aagaagatct agcggatttt
    33601 gaagaaaaga ttgaagatgc aattattaaa aacaaacgag tcaatttttt agactcctta
    33661 tttgatccat tcattacctt gattatcggt ctgtcttatg ttgcaacaat tattttaggt
    33721 ggaaagtatg tcttggaagg cacgatctcg atcgggcaga tggtctcatt tattagttat
    33781 atcggtatgc ttgtttggcc gatgtttgcg attggtcgat tgttcaacgt attggaaaga
    33841 ggaaatgctt cttatgatcg cgtcaatgaa cttttgcatg aaaagacgca tatcgtggaa
    33901 caactgggtg cagtgcagac cccggcaaaa ggcgagctga atgtagctat ttcctctttt
    33961 aattatccga aagaagaatc accagcatta gaacaaatca agtttgcgat ccaagaagga
    34021 gaaacattag gtattgttgg gaaaactggt gcgggaaaaa cgacgatcat caaattactg
    34081 atgcgtgaat acgatgatta tcaagggaaa atccaatttg gcggacgttc gatcaaagaa
    34141 tattcattga atgctttatt aggatcaatc ggctatgtgc ctcaggatca cttcttgttt
    34201 tcgatgacca ttcgagataa tatccgtttt tctaatccaa aactttcaga agaagaagta
    34261 gagcatgcgg cttctttagc atttatcaac aatgaaatca aggaattacc aaaaggttat
    34321 gataccttag ttggagaacg tggagtctct ttatctggcg gacagaaaca aaggatttcg
    34381 attgctcgag cattgatcaa aaatcctgaa ttattaatat tagatgatgc actctctgca
    34441 gtagatgcca aaacagaaga agctatcttg cataatttaa aagaaataag aaaagagaaa
    34501 acaactatca ttgcagctca ccgtttaagt agcgtgatgc atgcaaaaga aatcattgtt
    34561 gtcgatcaag ggaaaatcat tgaacgagga acccatcatg agctgcttgc actaggcggc
    34621 tggtatgaac aaatgtggga gaaacagcaa ttagaagcga agatagaagg aggcgaggcc
    34681 tgatggatga aaattatcaa tcggaatgga caaaatcgat tccacttaaa gaacagaaaa
    34741 agatcatcaa acgactgatg aagttcgcca aaccttttag aagaacgttc atcgttgcta
    34801 tcttatttgc gtttgctttg tctgtgatca atgtcctatt gccaaggatc atccaaactt
    34861 ttatggatga tcatctggct aaacagtcgg ctacgacaca agttattttg ttttttgctg
    34921 gtatgtactt atttggagtc atagtaaaaa gtatcatttg gtttttccaa tggtatttgt
    34981 attccatggc ctcattgaag acctatcagt atattagggt aaaattattt gaaaagctgc
    35041 atacactagg tatgcgttat tttgatcaga caccggcagg atcaaccgtt tctcgagtga
    35101 cgaatgacac agaaacgcta tttgaatttt ggtacgtctt tttgatggta ttgactggga
    35161 tctttgcagt cgtttcttca ttccttgcca tgtttcaaat caatggagaa attgccttgt
    35221 ggactttggt gtttttgccg attttaggaa tcgtcatctg gtattaccag aaattcagtt
    35281 ctcgtattta ccgaaatatg cgggaaaaat taagtcagct gaacacgaaa ttgaatgaat
    35341 cgatttccgg catgcgtatt atccagcagt ttcgccaaga agctcgttta gctaaagaat
    35401 ttgaacaagt gaacaatgag tatttagaca cgcgttatgc gatgatcaaa acgaattcga
    35461 ttctgttaag tccgatcatc aattttctat atgcattggc gattgcgttg gtgttgactt
    35521 tgtttgggat cgatgcgttg cattctcccg tagaagttgg actgatctat gcatttacga
    35581 cctatgtcca gggatttttt aatccaatgt ctcaaatgat ggactttttg agcatcttca
    35641 cagatgggat ggttgctggt agtcgtatct tgaagattat ggatacggaa gaatatgcac
    35701 cacaacaaaa ccctggagca gatcaaatga ttacttcagg taagattgag tttcgcaatg
    35761 taagcttttc ttatgatgga gagcacaatg tattaaacaa tatctctttt gttgctaatc
    35821 cgggagagac cgttgcatta gtgggacaca ccggaagcgg aaaaagctcg atcatcaacg
    35881 tattgatgcg gttttacgaa ttttatgaag gtcagatatt gattgatgat catgacattc
    35941 gagaatatcc aatggaagag ttacgtaaga aaatggggct ggtcttgcaa gatgctttct
    36001 tgttttatgg ggatatcgca ggaaatattc ggatgatgaa tccagctatc acagacgaac
    36061 agatacgcca tgcggctgag tttgttcaag caaacaaatt catcgatgaa ttaccaaaag
    36121 gatatcactc aaaagtgatc gaacatggtg ccagctattc tagtggacaa cgacagctga
    36181 tcacttttgc gcgaacaatc gtgactgatc ctaaaattct agtattggat gaagcaacag
    36241 caaatatcga tactgaaaca gaaggattga tccaagaagg attggcaaaa atgcgtcaag
    36301 gacggacaac gattgcgatt gcccatcggc tgtcaacgat tcgagatgca gaattgattt
    36361 tagttttaga caaaggtcat atagtagaac gcgggaatca tgaaacactt ctagcgcaaa
    36421 atggtttgta tgcagatatg tacaaactgc aaagcagtgg tatataaaag atgttgtgac
    36481 agtagaattt tgacctaagc actagagtga aaataaaaca tagcttctca tatttttcat
    36541 ctgtgagagt tagtgtcgta gtcaccagat ttaaaaaaag attcgacagc tagtcagaag
    36601 tgcctaatag ccactaagaa agcgttgaca aaagcatttg ctttcgataa tataaagaaa
    36661 acgatacaac ttggatagtg attgcctgat gcaagctaaa cctgatgagt ctcccatggt
    36721 ttcatggacg gctttttggg tttttctttt tgttttggat acagccctat acatcatgga
    36781 aaggagcaaa acatgctgat tgagaaagta ctgaataaca acgtcgtaat aaccagggat
    36841 gaaagaaatc aggaaatgat cgtgatggga aaaggtttgg cattcaaaaa gaaagtgggt
    36901 gacccgattt ttgactcatg catcgacaaa aaatttcagt tgacaaatga cgggctttca
    36961 caacagttcc aagaattggc acaagcgatt cctcttgagt acttagaaat ttcttgtgaa
    37021 atcattgaat atacaaaaga ggctctacaa gtccagttga atgattctgt gtatattgca
    37081 ttgacggatc atttgtatac ggctatcgaa cgtcacaaaa tgggaatatt tgtaccaaat
    37141 gtattgctgt atgatatcaa acaactattt cctaaagagt atgccatcgg gaaaaaaaca
    37201 gtgaagaaaa tctcggataa ataccacatc aagttatcag aagatgaagc cggatttatc
    37261 gcattgcatt tagtcaatgc acaaggcatt caaagccagc aagacaatac ctacctgttt
    37321 actataacaa tacaggatat tctaaatatc gtccgctatt actataagat tacatttgat
    37381 gaagattcag tccatttttt tcgattcgtc acacacttac gtttttttct gacacgcgtc
    37441 ctagatggag aaaaatcaga agtgggagaa gtttctgagg aattgtttca ggttattcaa
    37501 aaaaaatata aggatacgtt tggttgcgtt gagaaaatag cagacttttt agccgagacg
    37561 ttcaactacc agatgtccac ggacgaaaaa atgtatttaa cgatccatac tgcgcgttta
    37621 gtgcatgaga atcgttgtaa ataaaaaaag gaggataaat taacaagaac gtatgaaata
    37681 agggtagtga cattcagttg gctaaacctt ccactaataa attggaggga atagaaatgg
    37741 gaaaatacca agaattagcg agaaaaatcg tagaaaatgt cggcgggaag gaaaatatca
    37801 atggtttgac acattgtatc acaaggcttc gtttcaaatt aaaaaacgaa gaaaaagcaa
    37861 atgatgagat cttgaaaaat atggacggta ttgtaacggt aatgcgagcg ggagggcagt
    37921 atcaagttgt cattgggaat catgtgccag tcgtgtttga agaggttata aaagcaggaa
    37981 accttacttt tgatgaggct gtttcaacag aaaaaatgag accgtttgat atgcttattg
    38041 atggaatatc gggctgtttc cagccattcc ttgctatcct agctgctggc gggatgattc
    38101 gtggactgac agcattcctt gtcttcttag gcgcgtttga ccgcggttct ggcacatttg
    38161 ttatgtttga taatatcggt gattctgtat tccaatttat gcctgttatt atcggtttaa
    38221 cagctgcccg taaatttaaa gtaaatgaat tcgtagggat gttgcttggc gcagcattaa
    38281 tgaatcctag cttgtcgctg gaagctttga gtggtgcagc agaagcacca ctaacgacca
    38341 tattttctgg cactattttt gaagcaccta tttatcaaac agtattcggc attccctgga
    38401 ttgcgcgtaa ctacgcttct tctgtcattc ccattatctt tattgtgttg ttagctagcc
    38461 aaattcagaa accaattaaa aagttagtac ctgagatgat cgcgaatttc ttcgttccat
    38521 tcttcaccgt tcttattaca atgcctttag gttttttact cgttggacca gtattcacat
    38581 tcgcaactga tattttaatg gctggatttg aaacactttt agcactttcg ccagttattt
    38641 atggagcaat cgtaggcttc ttctggcaaa ttctggtgat gtttggtctc cattgggcaa
    38701 tcgtaccaat gggattgatg caattttctg tgaatggttg gcaaaatatt atgacacctg
    38761 ttgcagtagt gtcttttgga caaactgcag cacttaccgc actctacttt aagcttagaa
    38821 atccaaaaga caaagcaatt gcaataccag ccattgtttc tggtattgtg gggattacag
    38881 agccagctat ttatgggttt acattaccac gtaaaaaaat atttattttt acctgtgtcg
    38941 gtggtgcgtt agggggcgcg tatagtgggt taatgaattt aacttcttgg aatcaaggtg
    39001 gactaggcat ctttacaatt cccaactata ttagaccaga tggtgattta acagatgtga
    39061 ttaatgtatt gattgggatc gccattgcga tggtggtatc ctttacgctg acttttttct
    39121 tttggaagga tgaagcaggt gaaacagatg atatacagaa aaaatcaggt aaagaaattg
    39181 taaaaacacc gatacaagga caaatcgctc cactaaatgc agcaaaggat gcagcttttg
    39241 cacaaggaac actaggtcga ggaatcttga tctaccctga aaaaggagaa gtacgtgccc
    39301 cttttgacgg aacagtcatg accctgttcc cgacgaaaca ggcaattgga atggtttctg
    39361 aaacagggct ggaattgctg attcatgtcg gattggatac tgtgcagttg gaagggaaat
    39421 attttgaaag cttagtgcag caaggataaa gagtgaaaca aggcgatctg ttgctacgct
    39481 tcgacaaaaa gaaaatcgaa aaggaaggat acagtttaga aacacctgtg atcgtcacca
    39541 attacacgga ttatttggat attttggaaa accaagggga gacggtcggg ccagacgatg
    39601 cacttatcac cgcactgacc tgaacggtta ggtgaagaat ggactaggct taactattct
    39661 ttttattcca cttctttatt ttcaaaattg aaaggctctt tcatgatggc tgagctgata
    39721 gggcgtttat cgaatttttg accgacagaa attagatgga ctaaattctg ttgataattt
    39781 tctgcaaaaa cacaactata taacacgtct agaagataac aaattgatgt attgatcgta
    39841 aaattaccaa tttttgaata cagtcgctcg cgtgtcgtaa gtcgcaaagc ggctgtactc
    39901 attgcagata gcgttgaatc accgatactg gtcaaagcta aaattggaac atgattttgt
    39961 ttcaatattt ctgcggtacg aaggatcaca ttgttttcac ccgtataaga aatcagaatc
    40021 ccgcacatat cactcgtgca attgtacgct tcatagaatg attcaccaaa agtggtgaca
    40081 atcgtgacat ccattttgat gcgcttcatt ttcagcatga aatcttgaga aatcaaagta
    40141 ttcgagttac tggcgaaaat cacgattttt tttgcttgaa gcattagttg tttggctttt
    40201 tgcagttggt catgatgaat gagagaaagt gtatcatgaa tcgtcatctg ttctaaagaa
    40261 gccattttat ttgcgatcgt cataataccg tctgtggtgg aaaaaggaag attcgcatct
    40321 acgtcttgaa aattcccttc aagataggct gtttcagcta aaaaagcatt acgaaaactg
    40381 acccagccat caaaacccat tttttttgct acacggatga gtgtagaggg gtggacataa
    40441 catcgctcag caatcgcttg gatggtcatt tcgccaagtg ctggagaatc gcttaagaga
    40501 aactggatca cttcattttc tgaaggagaa aaggagattt ctttgatttt ttcacgtaat
    40561 aacatgtaga taccttcttc ttattttttt acgtaaatta taactttttt gaaaatgatt
    40621 tgtaaacgga tttatactat ataacttaaa atagacattt tttgacaaga tatttacaaa
    40681 aatccactag aaaacagtaa attttgtaaa tgttactgga aagaaacaga tttctattgt
    40741 ttgttttgtt tacaaaaaag ataatgaaag tgtaaaaaag taaaggagtg agaacatggc
    40801 aaaaggaatc aaaatcgcta cgattggtgg aggcagcagt tacacccccg agttgatgga
    40861 aggatttatc aaaagatatg aggaattacc gatacgagaa atctggctcg tagatgtaga
    40921 agaaggaaaa gaaaagcttg agatcgttag tcagatgtct cagcgtatgt gggatgcttc
    40981 tccgtatgaa gtcaagatct atgcaactct ggatcgggaa gaagcattga aagatgcgga
    41041 ttttgttacg actcagtttc gtgtcggtca attagatgca cgaatcaaag acgagcggat
    41101 ccctagttat tatgggatga ttgggcaaga aacaaatggt gctggcggga tattcaaagc
    41161 ttttcgtacc gttccagtcg ttttggcaat cgttgaggac atgaagcgtt tatgtccaaa
    41221 tgcttggctg atcaatttcg cgaacccaag tgggatggtg acagaagcag ttatcagata
    41281 tggcaaatgg gataaagtga ttggattatg caatgtgcca gtgatggcga tgatgacaga
    41341 accagcaatg cttggcaaga aaaaagaaga gctgatctac aagtttgccg gtctaaatca
    41401 tttccattgg cacaaagtag cagataataa aggaaatgac cgcacggcag aattgattga
    41461 tcgtttatac gatgaagaga atggattacc gaaaaatatc tttgatatcc cgtttttcaa
    41521 agaacaatta aaacaaatga acatgatccc ttgtggctac caccgctatt actatcgtga
    41581 agaagaaatg ctggaacatg ctttagaaga atatcaaacg attggtacaa gagcgcagca
    41641 agtgaaagaa acagaagcag agctatttga attatacaaa gatccagaat tagaccataa
    41701 acctgaacaa ttgcaaaaac gaggtggcgc ttattattca gacgctgcct gtgaaacgat
    41761 tgcttcaatc tatggaaata aagaaacaca gatcgtggta tctacaaaga atgatggagc
    41821 tgttcctgac ctgccggcag attgtgtagt agaagtcact gcctatgttg gcgcacaagg
    41881 agcgagaaat gtagcgtttg gttctcttcc tgctgctgaa agaggctggt tgcaagtaat
    41941 gaagaatatg gaattgctga cgatcgaagc agctgtgact ggtgattatg ggacagcttt
    42001 acaggcgttt acgatcaatc cacttatacg atcaggaaag acagcaaaac ggatcatgga
    42061 tgagttgttt attgcacacc agcagtattt accgcaattc aaggagacaa tcagccgatt
    42121 aaaagaagaa ggaatcgaag ttcaagatga actggcaaga gaattatcag gtaatacaca
    42181 tataatggag gaagtataat gggattcaga aaagattttt tatggggcgg tgctacagca
    42241 gccaatcaat gtgagggcgg ttatgatgaa ggtggacgcg gactagcaaa cgtagatgtt
    42301 gtgccaatcg gaaaagaccg ttttcctgtt atcaccggaa agaaaaaaat gtttgaattt
    42361 gatgaggatc atttttatcc agcaaaaatt gcaatcgata tgtatcatcg ttataaagaa
    42421 gatattgctt tgtttgcaga aatggggttc aagacttatc gaatgtccat cgcctggagt
    42481 cgtatttttc caatggggga tgagaaagag ccgaatgaag ctggcttgaa attttatgag
    42541 gatgccttta aagaatgcca aaagcacgga attgagccac ttgtgacgat tacgcatttt
    42601 gattgtccaa tgcatttgat caaagaatat ggtgcgtggc gttccagaga actcgttggt
    42661 ttttatgaaa atctgtgtcg agtcatcttc aatcgctata aaggacttgt caaatattgg
    42721 ctgacattca atgagatcaa tatgatcctt catgcaccat tcatgggcgc agggctgtat
    42781 tttgaaaaag gagaaaatga agaacaaatc aaatatcaag cggcacatca tgagttggtt
    42841 gcaagtgcca tcgcaacgaa gattgctcat gaagtagatc cggagaacaa agtaggctgt
    42901 atgctggcag caggagcata ttatccatac acttgcaatc ctgaagatgt ttgggcttct
    42961 agaaaatcag atcgagaaaa ttatttcttc attgatgtcc agtcacgagg agaatatccc
    43021 gcttatgcat tgaaagaaat ggagcgaaat ggcattcaga tcgaaatgga accaggggat
    43081 gccgcattgc tgaaagaaca tactgttgat tttatctctt tttcttatta tgcttcccga
    43141 gtttcgacta ccgacaacga actcttggaa cagactgcag gaaatatctt tgcttctgtc
    43201 aaaaatccat atctgcaagc aagtgaatgg gggtggcaaa ttgatccatt aggcttgaga
    43261 ataacgatga atgatattta tgatcgttat caaaaaccat tgtttattgt agaaaacggc
    43321 ttaggtgcag tcgatacgcc agatgaaaat ggttatgtgg aagatgatta ccgaatcgat
    43381 tacctggcag cgcatatcca ggcgatgaag gatgcagtag aactagacgg agtcgatcta
    43441 ttaggctata cgacttgggg atgtattgat ttagtatcag ctggtaccgg tgagatgaag
    43501 aaaaggtacg gattcattta tgtcgatcgt gataaccaag gaaatggcac attagcccgt
    43561 tcaaagaaaa aatcattcaa ctggtataaa aaagtaattg attcaaatgg agaaaatctt
    43621 tcgaacgatt gatcaatgat ttataaaagt ctattgtttt tcagcggatt tttcgagtat
    43681 aataaagcta ttcgagaaac gcaagcagaa caatagactt tttttcgcat tggctgtaaa
    43741 gtatgcttac ttgattaagt aaaggagaat ctacatatgt atgatgtaat cgttattggc
    43801 gcaggtcctg ctgggatgac tgctgcttta tatgcttcac gttcaaactt atccgtattg
    43861 atgatcgaac aaggtgcccc tggcggccaa atgaacaata cggcagaaat cgaaaattat
    43921 cctggattcg attcgatcat gggccccgat ctagcttata agatgtatga aagtgtctca
    43981 agatttggga cagaaaatgc ttatggaatc gttcaagata tcaaggatca cggcagccat
    44041 aaagaagtca tctgtgaaga taaaagttac gaaggaaaaa cagtcattat cgcaacagga
    44101 tgtatccacc gtaaattagg cgtacctggc gaggaagaat ttgctggacg aggcgtttct
    44161 tactgtgcag tctgtgatgg tgcgtttttc cgtaacaaac gtctagtagt catcgggggt
    44221 ggggactcag cagtagaaga agccatctat ttgacacaat ttgcttctga agtagtgatc
    44281 gtccaccgta gagatgaatt acgtgcacaa aagatcatcc aagaccgtgc atttgcaaat
    44341 gaaaaaattt cctttatgtg ggatacagtc gttgacgaaa tcgttggaaa cgaaatggta
    44401 gtgacaggtg tgaaagcacg taatgtcaaa accggcaatg aatttgattt agcagcagac
    44461 ggagtattca tctatgttgg actggatcca ttaactgcac cttttagaga agcaggtttg
    44521 acgaacgaag ctggctggat tccaacagac aacgaaatga aaacgaatat gccaggcgtt
    44581 tttgccatcg gagatgttcg tgaaaaagat ttgcgacaaa tcactacagc tgttggtgac
    44641 ggcggtattg ctggacaaca agtgtataaa tacttggagt ctttagaaga aacaacagca
    44701 taagtttaaa aagagtagaa gaacgggttc aattgttctt ttactctttt ttttcgcgtg
    44761 aaatttactg aatctgattt atgcgaacac gtccaacacc ttttgataaa agaatccttt
    44821 cttgatttaa gcgttttttg gtcctcatac tttcatatct aataagtgaa tgctatactt
    44881 attaagaacc atatttcaga aagaggtgtt attttcatgt cttgggaaca agtgtatgaa
    44941 caatgggtac atgaagaaaa tttagaagaa aacttaaaaa aacaattaaa tgatctgggt
    45001 gaagatcctg aaaaacttga agatgctttt tatgcaccgt tggaattcgg tactgcaggt
    45061 atgcggggaa tcttaggacc tgggatcaat cgaatgaaca tctatacggt aagacaagca
    45121 acagaaggac tagcacgttt catggataca caaggtgaag aaacaaaacg ccgaggtgtc
    45181 gctatcgctt atgactcacg ccatcagtca ccagaatttg caatggaagc agccaaaaca
    45241 ctagcacatc acaatatccc gtcttatgta ttcgaaagtc tgcgtccaac accagagctt
    45301 tcttttgcag ttcgtcattt gaagacgttt gcgggaatca tggtaaccgc atctcacaac
    45361 ccagcggctt acaatggata taaagtctac ggagaagatg gcggacaaat gcctcctgca
    45421 gacgcagatg cattgacgaa atatgtccgt gaagtcagca atccgctaaa agtcgaagta
    45481 ttatctgacg aagaagctaa acatagcggg ttgatcacga tcatcggaga agaagtggac
    45541 gctgcttatc tagaagaaat caaagcagtg acgattgatc gtgaattggt tgaaacaatg
    45601 gggaaagatt tgaaacttgt gtacacgcca cttcatggaa cagggaaaat gttgggcgaa
    45661 cgtgcgttga aacaagctgg attcgaacaa ttcgtactag ttcccgaaca agctgtggca
    45721 gatccagatt tcactacggt caaatcgcca aatccagaag aacattcagc ttttgaatat
    45781 gctatacgac ttggagaaaa agaaggagct gacttgctga ttgcaacaga tcctgatgca
    45841 gaccgtttag gcgcagcggt tcgtttgcca gatggttctt atgaagtatt gactgggaac
    45901 caaatcggag cattgtttgc tcaatatatt ttggaagcac atcaatcaag aggcacttta
    45961 ccagaaaacg cagcggtatt gaaatcaatc gtttccagcg aattgcctac agcaattgcg
    46021 gaacactacg gtgctaccat gttcaatgtg ttgacagggt ttaaattcat tgctgaaaaa
    46081 atccaacaat ttgaagaaga ccattcacat acttttatgt ttggattcga agaaagttat
    46141 ggttatttag ttaaaccttt tgttcgcgat aaagacgcga tccaagcatt gcttttatta
    46201 gcagaagtag cagcttacta taaaaaacaa ggcaaaacgt tgtatgacgg cttgcaaacg
    46261 atttttgaaa cctatggcta tttcgcagaa aaaacgattt ctgtgacaat gagcggacaa
    46321 gaaggttctg cgaaaatctc tgctttgatg gaaaaattcc gtaaagaagc accgacagaa
    46381 tttgccggag caaaagttat tcaaacagaa gactttaaag aattgacgag aacagaccgt
    46441 gaaggaaacg tagaaaaact aacaacaccg ccatccgacg tattgaagta tacattggaa
    46501 gaaaatggct ggatcgctat ccgcccttca ggaactgagc cgaaaatcaa gttttatatt
    46561 ggtgtaaaag gaagctctac agaagacgca gccgaaaaag tagcctctta tgaagcgatt
    46621 atcaaaaaga tgacagaaga ataaaaaact tgagactgat ggaaaaagtg gtaaacttta
    46681 gaagtcagtc ttttttcaag ttggaggtga atggattgcc agacacgcaa aaatcaaaag
    46741 tagagaaagt cagtcaatta ttcaaaatgt tgagtgatcc cactcgtctt aaaattctgc
    46801 tatatctgaa agacggggaa caaaatgtga cagcaatcac ccaagcagtg gaaatggagc
    46861 agtctgctgt atcgcatcaa ctacgtttat taagagaaaa tcatgtcgtc aagtctcatc
    46921 gcgagggaaa agcgattttg tatagtcttg atgattctca tgtactggat attttgaacc
    46981 agacactcaa acatgtggaa catcaataac gataggtgaa aatagataaa cactggagtg
    47041 tgacaacgaa gaaatttcgt tgtcacactc cagtgttttt taagaaaaag acaaaaatga
    47101 ttaatcgctt ttttctgttg tttcagttcc ataataacta tgatcttcta ggtctaaagc
    47161 attcaaaata agcgaagccg tttcttcaat agataaagat gccacattga tcactacaca
    47221 tcctagtttt tcataaagat cattcgcaaa atcgagttca gctctaattt tttcgatatc
    47281 ggaataagca gtatcagggt ttaatccgta agagcgcatc cgttctttac ggatattatt
    47341 taaaatatct gggttattcg ttagtcccac gattttttta ggatcgattt cccacagctg
    47401 tttgggaata tgtgcttggg gaatcaaagg taaattagca acttttaaat ttttatttgc
    47461 taaaaacaag ctcaaaggcg ttttggaagt tcgggatacg cctagcaaaa cgacatctgc
    47521 ttctaaaaaa ccacgaggat ctttcccatc atcgtatttt acagcaaatt ccattgcttt
    47581 gatccgtttg aaatagttct cattcaataa atggagggca ccaggttgcc gaattggctt
    47641 gataccagtt aaacgttcaa tttctgatat cggtggcgtc atcacatcga aactaaataa
    47701 ttgatgttct tcacaaaaag aacgtgttat ctcgatcagt tcttgattga ttaatgtatg
    47761 caggacaagt gctttttctt ctaatgcatc ttgcaatgcc tgccttaatg ttttttcatc
    47821 tgtcacgaac gttctgcgga ataggttgaa ctcaacggta ggatactgag ccatagtagc
    47881 ttgcgctagt tttgtcgcag tttctccagc agagtcggag atgacaaaaa aagtaacgat
    47941 ttctgtagaa tcggaagaca tggataaaac ctcacttgca ttttttttat gtttattata
    48001 ccatactcta gaatgcgtac ttcgtaatca ttttgatttg gaggaataag tcatgaaaaa
    48061 acagacattg atcgaacaat cattagaaac attgaaaaat aacggtttta aatatactaa
    48121 aaaaagagaa gcattgttaa cttatttagt gaagcgtaac cgctacgtat ctgctaaaga
    48181 agtttttgac tatatgaatc aggaattcaa aggggtaagt tatgatacga tttaccggaa
    48241 tctccatgat tttgcagaga tcgatttact ggaagaaacc gatttaaatg gtgaaatgaa
    48301 gtttcgtttt cattgctgtc agggggaaat cggccatcac catcatttta tctgtacagt
    48361 gtgcggaaca acgaaagaaa tccatatgtg tccaatgaat tatttcgaag aacagctagg
    48421 tggatgcacg atcgaagggc atagattcga gattttcgga agatgtgaaa attgtcaata
    48481 aggatttaaa taaaataaca tttgataatt taaaaaaaaa gcaagatttc cttgacggtc
    48541 aaaacagcat tagatataat atttacagga cagtcttgtt ttttgagata tatttcaata
    48601 acaattacgg ttacttttta agctcggagg gagggaatta acatgtcaaa aacagtcgtt
    48661 cgcaaaaacg aatcacttga cgatgctctt cgtcgcttca aacgttccgt gtcaaaagcg
    48721 ggtactttac aagaatctcg taaacgtgaa ttctacgaaa aaccaagtgt aagacgcaag
    48781 aaaaaatctg aagcagctag aaaacgcaaa aaattctagt tctgtgatga attggagttg
    48841 gtagtgtgtc actactaagt agattaaacg atgacatgaa agcagcgatg aaagcgaaag
    48901 ataaggaatc tttacaagtc atccgaatga tcaagtcatc tatacaaaac gagcagatta
    48961 aagaaggtca cgacttgaca gaagaagaag aactaacggt gttgtctcgc gaaatgaaac
    49021 agcgacgtga ttctttgcat gagtttgaag aagcaggacg tgacgattta gctgaaaaag
    49081 taaaaagtga aattgtaata gtcgaaaaat atatgccgga acaactttcc gacgaagaaa
    49141 ttcgacaact tgttcaagaa gcaatcactc aaactggtgc ttcatcaatg aaagaatttg
    49201 gcaaagttat gggtgcaatt atgcctaaag tcaaaggaaa agcagatgga aaccaagtga
    49261 atgcgattgt gaaagaacta ttacaatctt aattttatca tttaaactgc ggacagtgat
    49321 tgtccgcagt tttttgtgtt cgtaaaaaaa gaactaaaaa acagctagaa gaaagaataa
    49381 gattctttct tctagctgtt tttttcctgt ttaatatctc ttatgttaaa ttgtgtttga
    49441 tttggttatt cttctggtag ttcatttgct tcttgtttat accctgatag gcgataggcg
    49501 taataaaaga gtaagctgcc agcgtaacca agtaacgttg ggaccatcca gccaagtcct
    49561 aatgcagcaa aaggaacgtg atgatgataa aatcctagta agcttgctac gacagtttgc
    49621 tgactcacta atgcaggcga atttgccaac atatccaata cggctggaac ggcagaaaag
    49681 aacatcgtta cttgatagac aaatggactc ttttggaaga attttgctgt taaggaaagc
    49741 aagattaacg caagtgacaa tggatacaaa agcatcaata ctggtaaaga ccattggatg
    49801 atattatcca atccggcatt cgcaacgaca aaagagacaa aagtcgttaa ccgcagccat
    49861 gtcatataac tgactttagg gaacagttta tggaaatctt gtgcaaaaga aacaacgagt
    49921 cccatagcgg tagtgaagac acctaatgtg accattaccc ctaagaagct agcaccgaag
    49981 ttacctaaat aaaaattagt gatctgagaa agtgcagttc cgccattttc tgataaaggc
    50041 atcttattca aactcatcgc accgagaagg accagaccaa aataaatcat gacttctaac
    50101 gcaacgctca ggctaccagc ttttgctgtt aattctgtga cttttttatc tttaaaacca
    50161 agatcacgaa ctgcatggac aaaagtgaca cttaaagcaa gtaaagctac tgcatcaacc
    50221 gtgttgtatc cttctaaaac gccatttgtc aaagcgttcg tctgataaga atctgcagca
    50281 gattggttta gattccccat gggttggaat aaagaaacga caaaaatgat agctaataat
    50341 gcaagaaaca aggagtttaa atactttccg acatatttga ccaaacgtgt tggatgtaca
    50401 gtaaggaaat aagcaagtaa aaagaaaaga gcagagaata aaaacatacc taatgaatca
    50461 tatttttcag gcaaaaaggg tttagcagcc atttcaaaag cagttgctgc tgtccgcggt
    50521 gtaccaaaga atggtccgat cgttaaatga acaaggatca agaacaaggc agcataccat
    50581 tttccgacag gtttagctaa gtcatacaga ccatcacttt ttgtcactac tacagctaag
    50641 atagctaata atgggaaaag agcgcctgag atggcaaatc ctacaccagc gctgatccag
    50701 tttgatccag ccatttgacc taagtggaca ggaaagatca gattacctga cccgaagaac
    50761 atcccgaaga taagtgaact gaccatcaat aattgcttcc atgaataagt ttttgtttct
    50821 tccataaatt tttcctccaa taattatcat tttgagtaac aaaataaacg ttgcagaaac
    50881 tagaaatgcc actaatatca gattccatga aaaccaccac cttaaaatat aaaaaaaagc
    50941 ccttttccac ttgggaaaag ggctctagat tctagaacgg taccaccttt tttcgcttaa
    51001 aaaagcttct tcattcacgg acgcttttcg ataaagttat ccgctagcct gataatggag
    51061 gcagccacaa tgatctacgt tttcgacatt gactcggaaa tgatgttcgt ctattcgatt
    51121 tattgtcatt tctcacttac atgactcact gggaataaat cgctgaatag cctactgttt
    51181 tcctcgacgc tttgctgtat atttatttgt tgacgttata atacacctgt tttttttgaa
    51241 atgcaagaat atttcaaata ttttgttttc tttttttgaa acaagaaaaa tacccagaga
    51301 atacttgcta agttagacgg aatacttgtc ctctatctta tgcatagctt acatgatcag
    51361 ctttttttat tcaaacgaga cctttagaga atggaagcag ctatttttta acgtttcttg
    51421 gtacttatgg ttaagcaact cttttagaac cttttcttat ttgctatata gtttcttctg
    51481 agtatggtaa aataggaaag aatgaatcga aaaaaagggg atgtctattt ttgacggaag
    51541 agtctagttc tttagagatt agattgacgg ctgctgatga tgtcagcatg cttttaggtg
    51601 cacacgacaa gcatataaaa ctgattgaag aaacaacaga aacaaccatc catacacgtg
    51661 gagaaatcat tcagatcatt ggcgaacaat cccagatttc cttggctgcc tcagtcattc
    51721 ggacattgca ggaattgatc aaacgaggaa tccatgtaag tacaccagat gtcgtgacgg
    51781 cactgaaaat ggggcaaaaa ggcacattag attattttgt tgaaatgtat gaggaagaga
    51841 tcgtcaaaga ccgtaatgga aaaccaatac gggtaaaaaa cagcggccaa aagaaatatg
    51901 tcgaatcggt agccaaacat gatatcgtat ttggcgtagg accagccggg acaggaaaaa
    51961 cttttcttgc agtagtatta gcaatcgctg ccttgaaaaa aggtgaagtg caaaaaatca
    52021 ttttgacgcg gcctgctgtg gaagcagggg aaaatctagg ctttcttccc ggggatctaa
    52081 aagaaaaagt agatccttat ctgcgtcctg tttatgatgc tttataccaa atttttggat
    52141 tagatcatac gaaccgattg atggaacgag gcgtgatcga aattgctcct ttagcttaca
    52201 tgcgggggcg gacattggat gatgcattcg ttattttgga tgaagcacag aacacgacag
    52261 ttgctcagat gaaaatgttc ctgacacgtc ttggttatca atcaaaaatg attgtcaatg
    52321 gagatacgag tcagattgat ctgcctcgag ggacaacaag tggtcttgtt catgcagaac
    52381 gaacattaaa gcaaatcaag aaaatcgatt ttgttaattt tgaagcaagt gacgttgttc
    52441 ggcaccctgt agttgccgaa atcatcaaag cttatgaaaa ggcagatctc catcaagaat
    52501 aagctttgct taggaggata agcacatgtt gaataaaaca ctgcagaaac tacaagaaaa
    52561 acttggaaag gcagcattgc ctttcctgct gttcttgttt tttatcatta tctgcggcgt
    52621 tacatttagt agtgtcaaac aaaaggccaa tgatttcaga gaaggccaag tagcagaaga
    52681 aagtatccgt gcgaacaaaa cgatcgagaa cacggaagaa accgaacaaa aaagaaaact
    52741 ggcagcagaa gcagtcactc cagaatacac ttaccaaaaa gatcttgcag atgaccagaa
    52801 caatcggatc aagcaactat ttgaattgat cgataaaacg aatgatgcca tcaacaaatc
    52861 ttatgacgaa aagattgata aggcaaagga agatgaaaat atcccaaagc ctactacaga
    52921 agagcggatc gccgcgttga agaaaaattt tgaaaatgtg aatgcagaaa atgttgcgtt
    52981 ttatcaaaaa ctaccgaaca atttttattc gacgatattt gaaatgactg aaactgagat
    53041 ccatactgtg agggatgaga gtctgaagct cattgacgaa cagatgagca aacaagttcg
    53101 tgaaagtgaa ttggaagctt ttaaacaaga agcagaagag caaatccaat atttgaatgt
    53161 cacaccagca caacaacaaa tgattcgcta cttagtggat caaggtatcg tagtgaatga
    53221 cgtattgaat gagaaaaaga cagaagaatt gaaacaatcc gcacgagaag ctgtacaacc
    53281 agtgatgatc ttccaaggag aaatcattgt tcgtgaaggt aatcaaatcg atgcttcagc
    53341 tatgaaaaaa ttggaattac taggactgac taatcaaaca acttcgattt ttccgctaat
    53401 tgccatggtt ttagctgttt tgcttcaaat cggcgtactg atttataatt cacttcaatt
    53461 ccaaaatgca ggagaaagaa caaaatacgt gcttttctat gtgacagcta tgtcagtcag
    53521 catagtgctg atgaaatttt tccaattatt ccaaacggaa caagctactt atatcccgct
    53581 gttttatccc gctgcttttg caccacttgt tttaagcttt ttcttgaacc gaagagcagg
    53641 gatcatggct ggtattttcc aagcagtatc tgcacttttc attttttatg attcgattgg
    53701 cacgaacttt ctaacgatta tcttgatgtc ttatgttttc tctgggctca tggcaacgat
    53761 tgtcagacgc aaacggatct cagaacaagg attatctgct gccatgtggg tcattatttt
    53821 tcctgtgatg atggatgtca ttttgatcgt ctatcaagga atgagctttg gtgatgcaac
    53881 gacttggtta atgcttgtct gcgggttcac tggtgcactc ttctcattct tgctgacgat
    53941 cggtctacac ccatatattg aattgatggt caatgatgac agtgtaattg tattaaatga
    54001 actcagcaat ccaaatcatc ctttattgaa acagttgctg gaagaagcgc caggtactta
    54061 tcatcatagt atgatggtag ctaatctaag tgctaacgct gtagcagaaa tcggcggacg
    54121 ttctttgcta actcgagtag cttgctatta tcatgatatc ggcaagatca aacatgctag
    54181 ctttttcgtt gagaacttgc cttcaggagc ggaaaacccg cacaatttct tattgccaga
    54241 agacagtaaa catattatct ttggacatgt gacagatgga gccaaaatat tggaagatta
    54301 taatatgccg caaatggtga tcgatatttg caaacagcat catggaacca cgctaatgcg
    54361 ttatttctac atcaaagcga aagaacgcaa tccggaagta acagaagagc aattccgtta
    54421 tcctggacca aaaccgcaaa gtcgtgaagc agggatcgtc aatattgctg acagttgtga
    54481 ggctgcagta cgggcaatgg atcatccgac gagcgaaaaa atcgaaagtt ttgtccataa
    54541 tcttatcgaa gagcgtattt ccgatggtca attagacgat agtggactga ccttgaaaga
    54601 aatccgaaaa gtggaaaaat cattgatcag cggtttaagt agcaccttcc attctcggat
    54661 caaatatcct aaaatgaaaa cagaagttga gaagataaag gaagaacaag aagaatagaa
    54721 agaagcgatg tataatggat attaccttta tggatgaaac aaaccaagtc tcagaagaga
    54781 aaatgaaaga aatcgatgac ttgctgcaat ttgcagcatc ttatttaaag ctgccagagg
    54841 aaacagaaat gtctgttact ttcatggaca atgcggcaat ccaagtaatc aatcgtgatt
    54901 accgtggaaa agatgcaccg acagacgtca tcagttttgc tctggaagaa gaaggcgaag
    54961 acgaaatccc agtgattttt gaagatggag agaatccatt gcctcgtgta ctaggagaca
    55021 tcatgatttc tattgaacgt gctgctgaac aagcagaagc ttacggccat tcatatgatc
    55081 gggaacttgg ctttttagcg gtacatggat tcttgcatat caacgggtat gaccatatga
    55141 caccagaaga tgagaaagaa atgtttggtt tacagaaaga gatcttggat gcctatggac
    55201 tcaaaagata aaaacgtaga gaaaaacaaa catttcctga tgtctgtgga atttgctgtc
    55261 caaggagtca aaaccgtttt tgaggaagaa cgcaatatga ggaaacatgt tgcgttcggt
    55321 atccttgctt tatttatggg cgtgattttc cagcttgatc ggatagagtg gttatggctg
    55381 cttttagctg tttttctcgt ttggatagta gagatcatca atactgtttt tgaaaatgta
    55441 gtggacatgt ttacggattt tcattttcat ccgattggga aaaaaatcaa agatatggca
    55501 gctggagcag ttttagtgac cgcttgtttt gctgtcatca ttgggttgat tttatttgtc
    55561 cctaaaatat atcatttgtt gtttaattaa aaaggagaac gtatatgcca gaacataaat
    55621 cagggtttgt cgcaatcgtc ggccgcccga atgtaggaaa atccacatta ttgaaccgaa
    55681 tcgtgggtca aaaaattgcc attatgagtg acaaggcaca gacaactaga aataaaatcc
    55741 aaggagtata tactacacca gaagctcagc tgatttttat cgatacacca ggaatccata
    55801 aaccaaagca tcgtctagga gattttatgg tagaagcagc ttacagtgcg ttgaaagaag
    55861 tggatgcagt cttatttatg atcagtgctg atcaaaaacg cggccgggga gacgatttta
    55921 ttattgaacg tctaaaaaac gtgcattctc cagtttatct gattatcaat aaaatcgata
    55981 agatccatcc agacgagttg ctgggaatca tcgaagatta ttcgacgcag atgccttttg
    56041 cacaagtgat tccgatctct gctacagaag gaaacaatgt cgaacgctta atggatgtat
    56101 tagtcagtga aatgccagag ggcccacaat attttccaga tgatcaagtg actgatcatc
    56161 ctgaatactt tatcgtttct gaattgatcc gtgagaaagt cttgttttta acacgagacg
    56221 aagtgcctca ttctgtcgca gtcgttgttg attcgatgaa gcgaaacgag aatgataaaa
    56281 tccatatcca ggccacgatc atcgtcgaaa gagatagcca aaaaggaatc attatcggca
    56341 aaggcggaaa aatgctgaag caagtaggaa cgaaagcaag aaaggatatc gaaaatcttt
    56401 taggagacaa agtatttcta gaactttggg tcaaagtcca aaaagactgg cgagacaaaa
    56461 aagtctatct gcaagacttt ggctaccgaa aagacgaata ctgatattct gaacttctta
    56521 taggttaagt atagaaataa actggataaa aatccgcacg ttgagaaaaa cggcggaaga
    56581 tcgaaacata aataatctgg atcggcagaa tctgcttttc cagatttatt ttttaggagg
    56641 gagaatatga gccagcttgc agaatcgaaa ggcttgattt tatctagccg taattacaaa
    56701 gaaaaagata aattagtcaa aatctttacg gaatcttctg gcaaaatgat gttttatatc
    56761 aaaggaatcc atcggcaaaa tcagccatta gctccggctt tgctcccctt taccgaagca
    56821 gtctatatcg gtaattttcg aagtgaaggt ttgtcgtttt taagcagtgt caaagacgtc
    56881 catgcgtttc ggaacatcca agaagatatc tttttgagcg gttatggtac ctatttgcta
    56941 aatctagtag atgcagcaat cgaagatcat caatatgacc cgcatttatt ccaatttacg
    57001 caacaagctc tgcagcggat ggatcaagga gatgatgcag aaatcatcac gaatatcttt
    57061 gaagttcaaa tactgcaacg cttcggaatc gcgccaattt ggacgcactg tgtcgtatgc
    57121 ggggaaacaa aaggaaaatt tgactattct tccaaatctg gcggtgtgat atgcgagaaa
    57181 cactggccaa tggacgaaca ccgttatcat gcagatcctc gcgctgttta tttcgttcga
    57241 atgttttcgg ctatttctta tgataagatc agtggaatcc acttgaaaga agaaacgaag
    57301 caggcaatcc gtgacctcat tgaccagttg tatgatgaat atgtcggtat tcatctaaaa
    57361 agcaaaaagt tcattgatca aatgaagtct tgggcacacg tattaaagac gccagagaaa
    57421 aatatgcctg aaaaagagat acctgaaaaa gaagaataaa tgaaagatac aacaaaaaaa
    57481 tcaaaactat ttgacaaata cagggacttc cgtctattat taaccgtaga ttaaatagat
    57541 cgttcactga gaggatctat tatataaaca agaaaactgc aaaatgaata cgtacattga
    57601 ccaagatcag taaaaaacac gtggtttagc gagttcggga gagtggaagc cggacccaaa
    57661 cgatttttga agaggcactt gcgagtacga taaatgaagc tgccgaatct ttattcggaa
    57721 gtagggtgga accgcgaaat tattcgtccc tatgtctgta atcagacata gggacttttt
    57781 gtcgttttct ttcctgtatt cttattgaca gcaacgctta aaaattgaaa ggggcaaaac
    57841 agatgagtaa aaaattgacc gtccaagata tgattttaac attacaaaaa ttttggtcat
    57901 caaacggctg catgctgatg caagcttatg acacagaaaa aggagcaggt acgatgagcc
    57961 catatacatt cttacgggca atcggaccgg aaccatggaa tgctgcttat gtagagccat
    58021 cccgccgacc agctgacggg cgttatggag aaaacccaaa ccgtttgtat cagcatcatc
    58081 aatttcaagt cgtaatgaaa ccttcacctg aaaatatcca agagctttat ttagaaagct
    58141 tgcgtctttt aggaattgat ccattagaac acgatattcg ttttgttgaa gacaactggg
    58201 agaacccatc gatggggtgt gccggtgtgg gttgggaagt atggcttgat gggatggaga
    58261 tcacacaatt cacgtacttc cagcaagtcg gaggactacc ttgtaaacca gttacttctg
    58321 agatcactta tggactagaa cgtcttgctt catatattca agaagttgaa agtgtctatg
    58381 atctggaatg ggctgatggt gtgaaatatg gcgagatctt caaacaacca gaatatgaac
    58441 attccaaata ttcttttgaa gtaagtgacc aagacttgct actaagcaac tttgaccgtt
    58501 ttgaaaaaga agctaaacgc tgtatcgatg aaaatcttgt tcatccagcg tatgattata
    58561 ttttgaaatg cagccataca ttcaatttat tagatgctcg cggtgcagta tccgtaacag
    58621 aacgagcagg ttatctttcc cgtatccgca acatggctcg tgctgtagcc aaaatctttg
    58681 tggcagaacg cgaaaaatta ggattccctt tattgaataa aaacacagca aatccagaaa
    58741 acatgaagga ggctgaataa gatggcaaaa gacttattac ttgaaatcgg gctggaagaa
    58801 atgcctgctc atgttgtcac accaagcatg aaacaattag aagaaaaaac agcaaaattt
    58861 ttagatgaaa accaattgac ttatgatacg atcgaaacgt tttctacacc gcgccgctta
    58921 gcaatcaaag taacaaacat tccagaacgt caagcagaca gtgaagaaga agtcaaagga
    58981 ccagccaaaa aaattgcttt ggacgcagat ggaaactgga cgaaagcagc tgaaggattt
    59041 gtccgcggac aaggtctgac gacagatgct atcactttta gagaattaaa aggcgtggaa
    59101 tatgtctatg taacgaaata cactcacggc aaagaagcga aagaagtatt gacaggttta
    59161 gcagatgtca ttaaaagcct cactttccca gtaacgatgc attgggcaga atacgatttt
    59221 gaatatatcc gccctattca ctggatcgtg gcattgttag atgacgaagt aatcccgttt
    59281 gagatcttag atgtcaaaac aggcagagcc tctcgaggac atcgtttctt aggcgaagat
    59341 gtgacatttg cgcatgcaaa agaatatgaa gaaaaattga atgaacaatt cgttattgct
    59401 tcaccgcaaa aaagacaaga aatgatcgtt acacaagcaa atgaattagc cgaaaaaaat
    59461 cactgggtta ttgatctaga cgaagattta ctagaagaag tcaacaattt agtcgaatat
    59521 ccaactgtct tttctggcga cttcgaagaa aaatatcttt ctgttcctga agaagtacta
    59581 gtcacttcta tgaaagaaca tcaacgttat ttcgaggtac ggaacgatca aggtatgctg
    59641 cttcctcatt ttatttctgc tcgtaatggt gatcgggtaa aattggaaaa tgtggttcga
    59701 ggcaatcaaa aagtattgac ggctcgttta gaagatgcgg aattctttta taatgaagac
    59761 aagaaagctt caatcgacca atacgttgaa aaactaaaaa ctgttacttt ccatgaaaaa
    59821 atcggtagta tctatgagaa aatgcagcgg gtacaagtca ttgcccgtat cattggcgaa
    59881 caagtaggac tatctgagga agaattgatg gatctgcaac gagctgctga aatctataaa
    59941 tttgatctag taaccaacat ggtaggggaa tttcctgaac tacaagggat catgggcgaa
    60001 aaatatgctc tgctgaaagg cgagaaacct gctgtagcaa cagcgattcg tgagcattac
    60061 atgcctatat ccagtgaagg agagctacct cagtctacag taggttctgt tttagcgatt
    60121 gcagataaat tagatagcat tttttctttc tttgcagttg ggatgattcc aagcgggtca
    60181 aacgatcctt atgccttacg tcgccaagca tacggtgtcg ttcggatcat cgaatcgaaa
    60241 aaatgggcct ttcctttgtc tgttctgcaa gaaacgatta gtgatgttat cagtaaagat
    60301 acggatcgtt tcggcattgg tttatcagaa gggcaacaac aggtgatcga ctttatcaaa
    60361 ggccgtctgc gccaattatt gacaacgaag aatattcgtc atgatgtcat cgaagctgtt
    60421 ttgaatgcag aacaaaaaga tctgacaaaa gtatttgcag cagcacaatt gttcaagcag
    60481 catcttgcag atgaagactt caaaccatcg atggaagctt tgacacgtgt cgtgaactta
    60541 gcgaaaaaag cggagcttga aaaacaatca gaagtagacc ctgaactgtt cgaaaatgaa
    60601 gcggagaaag aactgcacaa agcagtagag gcaatgaaaa aaagctttgc tgaccaaacg
    60661 atcaacgaaa attatgatca gcttgtaagc cttcgcccac tgatcgaaaa ttattttgat
    60721 cagacgatgg tcatggcaga agaaccagca gtacgtaaga accgattaac gcaattaagt
    60781 caaattgctg ccatggcttt gtcgctagca agtttagatc aaatcatcac aaaataattt
    60841 ctaatatgta aataaaccag agaataggca caaaa
//
LOCUS       DARBET010000008        60193 bp    DNA     linear   BCT 07-NOV-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4027
            SAMEA114569841-rid20112183.denovo.008, whole genome shotgun
            sequence.
ACCESSION   DARBET010000008 DARBET010000000
VERSION     DARBET010000008.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA114569841
            Sequence Read Archive: ERR12203666
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 60193)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 60193)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001972618.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001972618.1
            Genome Coverage       :: 132x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/07/2023 13:18:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,041
            CDSs (total)                      :: 2,975
            Genes (coding)                    :: 2,908
            CDSs (with protein)               :: 2,908
            Genes (RNA)                       :: 66
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 67
            CDSs (without protein)            :: 67
            Pseudo Genes (ambiguous residues) :: 0 of 67
            Pseudo Genes (frameshifted)       :: 26 of 67
            Pseudo Genes (incomplete)         :: 28 of 67
            Pseudo Genes (internal stop)      :: 20 of 67
            Pseudo Genes (multiple problems)  :: 6 of 67
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..60193
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA114569841-rid20112183.denovo.008"
                     /isolate="VRE4027"
                     /db_xref="taxon:1352"
                     /country="Denmark"
     gene            complement(114..473)
                     /gene="rplT"
                     /locus_tag="SCJ86_000597"
     CDS             complement(114..473)
                     /gene="rplT"
                     /locus_tag="SCJ86_000597"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723984.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L20"
                     /protein_id="HEG1168183.1"
                     /translation="MARVKGGTVTRKRRKRVLKLAKGYYGSKHTLFKTAKEQVMNSYN
                     YAYRDRRQKKRDFRKLWIARINAAARMNGLSYSKLMHGLKVAEIDINRKMLADLAVND
                     AAAFTALADQAKEALAK"
     gene            complement(526..726)
                     /gene="rpmI"
                     /locus_tag="SCJ86_000598"
     CDS             complement(526..726)
                     /gene="rpmI"
                     /locus_tag="SCJ86_000598"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001125942.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L35"
                     /protein_id="HEG1168184.1"
                     /translation="MPKQKTHRGSAKRFKRTGKGGLKRFRAFTSHRFHGKTKKQRRQL
                     RKASMVSSGDFKRIRQQLAKMK"
     gene            complement(798..1322)
                     /gene="infC"
                     /locus_tag="SCJ86_000599"
     CDS             complement(798..1322)
                     /gene="infC"
                     /locus_tag="SCJ86_000599"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010750600.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-3"
                     /protein_id="HEG1168185.1"
                     /translation="MTIAKDMMVNDGIRARELRLIGSDGEQLGVKTKAEALNIAEQAN
                     LDLVLVAPGAKPPVARIMDYGKYRFEQQKKDREARKKQKVINVKEVRLSPTIDLNDFN
                     TKLRNARKFLEKGDKVKASIRFKGRAITHKEIGQKVLNRLAEETADIATVEQKAKMDG
                     RSMFLTLAPKNDSK"
     misc_feature    complement(1366..1491)
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00558"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="ribosomal protein L20 leader region; Derived by
                     automated computational analysis using gene prediction
                     method: cmsearch."
                     /db_xref="RFAM:RF00558"
     gene            1642..2274
                     /locus_tag="SCJ86_000600"
     CDS             1642..2274
                     /locus_tag="SCJ86_000600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294161.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pentapeptide repeat-containing protein"
                     /protein_id="HEG1168186.1"
                     /translation="MKKIEPIPPSLPLLTKGEFYLEDEEIFSGLLINGGDQSYLDAKN
                     MVLRESHIQKLTMQKTVLRHFECSNVIFEKCDFSNLEWIGGSFHQVVFHQCKLTGTNF
                     AESYLRDCTFTDCIASMASFSSTNLKAVSFDHCQLEDSEFYEVTWKNLFLSDNQLTGS
                     NWFRTSLKGLDFTTNFFTAIALSQDSLAGLIVNQEQALVIAQALGLQIKD"
     gene            complement(2276..3181)
                     /locus_tag="SCJ86_000601"
     CDS             complement(2276..3181)
                     /locus_tag="SCJ86_000601"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium transporter CorA family protein"
                     /protein_id="HEG1168187.1"
                     /translation="MREKHYFNQDRNFWINVQQNDVLAVKNLKEEYGISDEMLTYSLD
                     KNERARVEYDSFDEALLLVSNVPHQQKVDNHYETSPIAFILKEDGLFTFTTPNTEYVI
                     RLIRSLLERMPDMSVYSLLFRTLFLISDSFFPLIEEVNSERQRLNLKLREKTTNKNLL
                     QLSDLEIGLVYLVTGTKQNVVLLEQIKALAIYRKLSEKEKEELDDALIEARQAVEMTN
                     LASQILDQLSGTYNNLLNNNLNDTMKFLTVWSLILTVPTIVTGFFGMNLQLPFTHSVF
                     GWGIALIISLVLSIWMLIALWRRIR"
     gene            complement(3266..3925)
                     /locus_tag="SCJ86_000602"
     CDS             complement(3266..3925)
                     /locus_tag="SCJ86_000602"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002356607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="deoxynucleoside kinase"
                     /protein_id="HEG1168188.1"
                     /translation="MSVIVLAGTIGAGKSSLTEMMAEHFDSQAFYESIDDNEVLPLFY
                     ANPEQYAFLLQIYFLNKRFASIKQAMKDDNNVLDRSIYEDSLLFHLNADLGRATETEV
                     RVYDELLENMMEELPYAAHKKHPDLLVHIRVSFDTMLERIEKRGRSYEQLSFDPSLYD
                     YYKELNRRYDQWYEEYKESPKIQIDGDRYNFVEDPQAKEEVLKMIEEKLAEIRQTKVA
                     I"
     gene            4241..5185
                     /gene="mvk"
                     /locus_tag="SCJ86_000603"
     CDS             4241..5185
                     /gene="mvk"
                     /locus_tag="SCJ86_000603"
                     /EC_number="2.7.1.36"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293366.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mevalonate kinase"
                     /protein_id="HEG1168189.1"
                     /translation="MANYGQGESSGKIILMGEHAVVYGEPAIAFPFYATKVTAFLEEL
                     DAMDDQLVSSYYSGNLAEAPHALKNIKKLFIHLKKQHDIQKNLQLTIESTIPAERGMG
                     SSAAVATAVTRAFYDYLAFPLSREILLENVQLSEKIAHGNPSGIDAAATSSLQPIYFT
                     KGHPFDYFSLNIDAFLIVADTGIKGQTREAVKDVAHLFETQPHETGQMIQKLGYLTKQ
                     AKQAIIENSPETLAQTMDESQSLLEKLTISNDFLNLLIQTAKDTGALGAKLTGGGRGG
                     CMIALAQTKTKAQEISQALEDAGAAETWIQGLGVHTYV"
     gene            5178..6155
                     /gene="mvaD"
                     /locus_tag="SCJ86_000604"
     CDS             5178..6155
                     /gene="mvaD"
                     /locus_tag="SCJ86_000604"
                     /EC_number="4.1.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374283.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diphosphomevalonate decarboxylase"
                     /protein_id="HEG1168190.1"
                     /translation="MFKGKARAYTNIALIKYWGKKNEELILPMNNSLSLTLDAFYTET
                     EVIFSDSYMVDEFYLDGTLQDEKATKKVSQFLDLFRKEAGLSLKASVISQNFVPTAAG
                     LASSASGLAALAGACNTALKLGLDDLSLSRFARRGSGSACRSIFGGFVEWEKGHDDLS
                     SYAKPVPSDSFEDDLAMVFVLINDQKKEVSSRNGMRRTVETSSFYQGWLDSVEGDLYQ
                     LKQAIKTKDFQLLGETMERNGLKMHGTTLAAQPPFTYWSPDSLKAMDAVRQLRKQGIP
                     CYFTMDAGPNVKVLVENSHLSEVQETFTKLFSKEQVITAHAGPGIAIIE"
     gene            6168..7253
                     /locus_tag="SCJ86_000605"
     CDS             6168..7253
                     /locus_tag="SCJ86_000605"
                     /EC_number="2.7.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341069.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphomevalonate kinase"
                     /protein_id="HEG1168191.1"
                     /translation="MIEVSAPGKLYIAGEYAVVETGHPAVIAAVDQFVTVTVESARKV
                     GSIQSAQYSGMPVRWTRRNGELVLDIRENPFHYILAAIRLTEKYAQEKNILLSFYDLK
                     VTSELDSSNGRKYGLGSSGAVTVATVKALNVFYALNLSQLEIFKIAALANLAVQDNGS
                     CGDIAASCYGGWIAFSTFDHPWLQEQETQHSISELLALDWPGLSIEPLIAPEDLRLLI
                     GWTGSPASTSDLVDQVHRSREDKMVAYQLFLKNSTECVNEMIKGFKENNVTLIQQMIR
                     KNRQLLHDLSAITGVVIETPALNKLCNLAEQYEGAAKSSGAGGGDCGIVIVDQKSGIL
                     PLMSAWEKAEITPLPLHVYSDQRKENR"
     gene            7250..8314
                     /locus_tag="SCJ86_000606"
     CDS             7250..8314
                     /locus_tag="SCJ86_000606"
                     /EC_number="5.3.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319476.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 2 isopentenyl-diphosphate Delta-isomerase"
                     /protein_id="HEG1168192.1"
                     /translation="MNRKDEHVSLAKAFHDKQKNEFDFVRIIHNPLPQIAVSDVDLST
                     QAVGFTLSSPFYINAMTGGSEKTKKINQDLAIVAREADLMIATGSVSAALKVPSLADT
                     YTIMRQEYPHGKIIANIGAGTSVERAKEAIRLFHADALQIHLNAPQELVMPEGDRDFT
                     NWKVLIQETQTAIDVPLIVKEVGFGMTRETLNDLAALGVHTVDISGRSGTSFTQIENA
                     RRSKRELSYLADWGQSTVSSLLEANEADTSMEILASGGIRNAYDIFKALCLGANAVGT
                     SGTVLTHLMNHGVEETIILMKQWQEELRLLYTMVGATNTAALHQQSLIFSGPVKDWCE
                     ARGIDLVKYGRRTEKSVGQK"
     gene            complement(8563..9366)
                     /locus_tag="SCJ86_000607"
     CDS             complement(8563..9366)
                     /locus_tag="SCJ86_000607"
                     /EC_number="1.1.1.69"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010741490.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gluconate 5-dehydrogenase"
                     /protein_id="HEG1168193.1"
                     /translation="MEFSMDMFRLDGKVALVTGAVYGIGFEIARSLATAGAKIVFNNL
                     NQESVDEGIAHYKEAGIDAKGYVCDVTDEEAVQAMVQQIKEDVGSVDILVNNAGIIKR
                     TPMIEMDAADFRQVIDVDLNAPFIVSKAVIPDMIEKGGGKIINICSMMSELGRETVSA
                     YAAAKGGLKMLTKNIASEYGQYNIQCNGIGPGYIATPQTAPLRETQENGERHPFDQFI
                     VGRTPAARWGEPIDLAGPSVFLASSASDFVNGHVLYVDGGILAYIGKQP"
     gene            complement(9386..10228)
                     /gene="kduI"
                     /locus_tag="SCJ86_000608"
     CDS             complement(9386..10228)
                     /gene="kduI"
                     /locus_tag="SCJ86_000608"
                     /EC_number="5.3.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019722412.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-dehydro-4-deoxy-D-glucuronate isomerase"
                     /protein_id="HEG1168194.1"
                     /translation="MQEMETRYTHSPEDIRHYSTEQLRKEFLVEKVFEPGKISLTYTH
                     NDRMIFGGVTPTEKELEITLDKELGVDYFLERRELGVINIGGPGFIEIDGQKEEMKKQ
                     DGYYIGKETRHVVFSSQDAENPAKFYISSVPAHHKYPNVKISIDQVKPMETGEGLTLN
                     ERKIYQYIHPNVCESCQLQMGYTILEPGSAWNTMPCHTHERRMEAYVYFDYANEDTRV
                     FHMMGKPDETKHLVVDNEQAIISPSWSIHSGVGTSNYSFIWAMCGENITYTDMDMVQM
                     DQLK"
     gene            complement(10244..11251)
                     /locus_tag="SCJ86_000609"
     CDS             complement(10244..11251)
                     /locus_tag="SCJ86_000609"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331990.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar kinase"
                     /protein_id="HEG1168195.1"
                     /translation="MGKVVTLGEIMLRLSTEQGTRIDSSERFAAHYGGGEANVAISLA
                     NYGHEAIFATKVPDNALGKAVKKHLQCYGVDTKQVLSGGSRLGTYYMETGSGERAASV
                     IYDRAGSSFAEMDHLEWSLEELFQGIDIFHVSGITPALSKKWQKMTKQVIEAAKKAGC
                     LISFDINYRGKLWTQQEASEVIHQLLPLVDICSAGKMDALYLLGIEKAPEAEEQPLIY
                     YYQKMQEKFPNIQVFYSTKRTVHSASENELKGTLWMNHEYVESQVHRITPIVDRVGGG
                     DAFAGGVLHGILSNMSPQEIIDFATAASALKHTIHGDCNQFSQEEVAQFLACGSGKII
                     R"
     gene            complement(11258..11902)
                     /locus_tag="SCJ86_000610"
     CDS             complement(11258..11902)
                     /locus_tag="SCJ86_000610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288242.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional 4-hydroxy-2-oxoglutarate
                     aldolase/2-dehydro-3-deoxy-phosphogluconate aldolase"
                     /protein_id="HEG1168196.1"
                     /translation="MKKIDILSRLKNAGVIAVVRGKSKEEALNACHAIIKGGLTGIEL
                     TFTVPQADQVIKELLSFYKDQPEIVIGAGTVLDAVTARLAILAGAEYIVSPSFDQETA
                     EMCNLYQIPYLPGCMTITEIKTALKSGVDIVKLFPGSAYGPSIISAFKAPMPQVNIMP
                     TGGVSLDNMKEWFDAGVVTVGVGGNLLAPAATGDFDKVTEVAQQYAAKMKEIKR"
     gene            complement(12002..12760)
                     /locus_tag="SCJ86_000611"
     CDS             complement(12002..12760)
                     /locus_tag="SCJ86_000611"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002351454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IclR family transcriptional regulator"
                     /protein_id="HEG1168197.1"
                     /translation="MENKKPYGTVLIKASKILDYLAENQDVSLQTIAKGVGMTSSTVL
                     KILDTLLMIGYVNKNSEKNYRLGAKLIRYANKNIEQIDLVELTLPFLEQLQQKIDETI
                     HLGVLDNNEILYVNKLDPKHQTIRMSSKIGITRPLYNSAMGKAVLAEFSEEQYTDYLE
                     KHPLIPYTEYTITNALRLKKEIKTVKETHIAFDDEEIERDIFCIGASIVKNEQIIGAF
                     SVSMPKYRLTDETKEQIISALKQTKSEIEQRIQK"
     gene            complement(12887..14038)
                     /gene="fucO"
                     /locus_tag="SCJ86_000612"
     CDS             complement(12887..14038)
                     /gene="fucO"
                     /locus_tag="SCJ86_000612"
                     /EC_number="1.1.1.77"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343318.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lactaldehyde reductase"
                     /protein_id="HEG1168198.1"
                     /translation="MSNRMILNETSYFGKGAIESIVPEFQQRGFGKAAVITDKGLIEH
                     GIATKVTELLDKAAIAYALYDGIVPNPTIQNVKDGVAFVKEAEADCLIAIGGGSPIDT
                     AKAIGIILTNPEFSDVISLEGVADTKNPCLPILAIPTTSGTAAEVTINYVITDEVNHR
                     KFVCVDPHDIPIVAFIDSDMMMGMPKKLAASTGMDAMTHAIEGFITKGAWEMTDMLHL
                     KAIEIIGHSLEASVDGDQNGREKMALGQYIAGMGFSNVGLGLVHGMAHPLSAWYNIPH
                     GVACAALLPTVMKYNKEYTGEKYREIALVLGIKGAAEMSLEDVREAACGEIDRLSKAV
                     GIPETISELGVKEADIPAIAEDALRDVCTPGNPRETTVEEIIALYQSLM"
     gene            complement(14120..14431)
                     /gene="rhaM"
                     /locus_tag="SCJ86_000613"
     CDS             complement(14120..14431)
                     /gene="rhaM"
                     /locus_tag="SCJ86_000613"
                     /EC_number="5.1.3.32"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288247.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-rhamnose mutarotase"
                     /protein_id="HEG1168199.1"
                     /translation="MVKKAFCMKVYPEKQEEYKKRHDELWPEMRTALREHGVIDYSIY
                     LDPSTSLLFGYLVIEDEKLWEKMANTSINQKWWKYMEDIMETNPDASPVSWELMPVFE
                     L"
     gene            complement(14433..15278)
                     /gene="rhaD"
                     /locus_tag="SCJ86_000614"
     CDS             complement(14433..15278)
                     /gene="rhaD"
                     /locus_tag="SCJ86_000614"
                     /EC_number="4.1.2.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325228.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhamnulose-1-phosphate aldolase"
                     /protein_id="HEG1168200.1"
                     /translation="MKKIDILQAPYVKEMIETTSNLYRLGWDERNGGNISYLLKEEEI
                     TPFLDPEKVIRRISMIFDASRLAGCYFIVTGSGRYFKNVASEPAENLGIVRVAEDGKT
                     LELLWGLENECLPTSELPSHFMSHIARLEVDPDNRIVMHCHASHLLAMSFTHELDERS
                     FSRTLWQMCTECLVVFPEGISIIPWMVPGTNEIGEATAEKMKETRLVLWPQHGIYGSG
                     KDMDEVFGLIETAEKAAEVYTYVKAQGPILQTITDENLWHLADAFGVTPKAGYLEEVH
                     TKAGV"
     gene            complement(15292..16548)
                     /gene="rhaA"
                     /locus_tag="SCJ86_000615"
     CDS             complement(15292..16548)
                     /gene="rhaA"
                     /locus_tag="SCJ86_000615"
                     /EC_number="5.3.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345777.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-rhamnose isomerase"
                     /protein_id="HEG1168201.1"
                     /translation="MNTIERNYEQAKEKYATIGVDTDAVLEKIQDIKISMHCWQGDDV
                     KGFLTPDGELTGGIMATGNFPGAARTPEELRQDLEKAYSLIPGKHKLNLHAIYLDTEE
                     SVDLNEIEPKHFEKWVEWAKKEEIGLDFNPTFFSHPMMKDGFTLAHPDKEVREYWIEH
                     GKRSRKIAEYFGKETGQTSINNFWVPDGMKDNPIDRFTPRERLMESLDEIFTEELDER
                     YVQDAVESKLFGLGAEAYTVGSHEFYMGYGLTRDKLVCLDAGHFHPTEVISNKLSSLA
                     LFSKGIMLHVSRPVRWDSDHVVLMDDELQEIAKELVRNELLEKTNIGLDFFDATINRI
                     AAWVIGTRNTQKALLKAMLEPVERLKEMELAFDFTSRMAYTEELKDFPYADVWNYFCY
                     KNQVPVGLDWLEEVQEYQKDVLELRK"
     gene            complement(16545..18005)
                     /gene="rhaB"
                     /locus_tag="SCJ86_000616"
     CDS             complement(16545..18005)
                     /gene="rhaB"
                     /locus_tag="SCJ86_000616"
                     /EC_number="2.7.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhamnulokinase"
                     /protein_id="HEG1168202.1"
                     /translation="MNPTFVAIDIGASSGRLMKSTRDAKGSLILEEVHRFKNGFRNKD
                     GHDRWKIDYLIKEILIGFEKLKQTGIDRLFAGIDTWAVDYCLIDEQGDRINDPIAYRD
                     KRTNGAVEQFSEIYSLDKLYEQTGIQIQPFNTLFQLFAEEKENLAKAHKLLLIPDYLG
                     YVFTGNMVTEKTNASTMQLLNAGTKEWEIELLEKIGVSKELFPPLTEPGTILGDLQKE
                     QFSKYDLPDVTFITVASHDTASAVLGTPGQGERWGYISSGTWSLLGIETKIANVSRNA
                     FSENYTNEWGAHNTVRFLKNIMGMWLIQEVARMQEYEYSYAELAELAAKEKPLQQVME
                     INDPRFLNPPNMIEEIQQYCFETEQPVPVSSGAIARCVYDNLALCYAQELIKVEHLTG
                     TKLEKLHIVGGGSNNRLLNQLTADYAGIIVEAGPSEATAIGNVAMQMLSTGQFSNIEQ
                     ARNVIKQSFPCETFYPDENKQNTMEWYVNKIKGVIV"
     gene            18158..19027
                     /locus_tag="SCJ86_000617"
     CDS             18158..19027
                     /locus_tag="SCJ86_000617"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309732.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family ligand binding domain-containing
                     protein"
                     /protein_id="HEG1168203.1"
                     /translation="MDYWEKANHSWSEDSKRVILTPTQRSRNLFFYIQEIGYFKAPIP
                     YYTERAHLPSYLIKFTLSGKGSLTYNDNQQQLKAGDLFFIDCQEYQRYQTISEEPWEM
                     DWIHLNGQNVQSFYQEFTKDGHNTFHSTGDPSENKIHHLITELIHMQQGTNARTDFQS
                     SVAIHELLNELILQKYQLDFEEEDIPAYVYKLKQYLDEHFKETITLDQLEQQFLLNKY
                     QLSKDFSKFIGSPPIDYLISKKLSYAKDLLRYTEFTVRMISEEIGIENFAYFSRLFKA
                     KTGLSPRDFRKFS"
     gene            complement(19153..20052)
                     /locus_tag="SCJ86_000618"
     CDS             complement(19153..20052)
                     /locus_tag="SCJ86_000618"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293388.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HEG1168204.1"
                     /translation="MLQKKISGEQVNTMVSDQYEIYHVKDVVSQNKTIYHHHDFYEIH
                     ATLKGEATFYLNGRQFTIKAGSVLLIHSNDLHRIVRQSSDVFERVYIFVTPSFLESRS
                     TPWSNLSACFQPMGEKRSRVLQIEQEVLKQKLSFIDHPPDKKIYGEDIKYEQALVEYL
                     IFLNQVVQKEESAQEQNAVVPNERVEQMLQYISKNLSHPLSLEEMEKQFFVSKYHITR
                     EFKKYTGFTFHQYVMKKKLIYAKHLLREYRSSSAVYSKSGFSSYPHFLKAFKREFGIT
                     PKEFLKRDTQKQLVHYEHFEDEQ"
     gene            complement(20217..21041)
                     /locus_tag="SCJ86_000619"
     CDS             complement(20217..21041)
                     /locus_tag="SCJ86_000619"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphate isomerase/epimerase"
                     /protein_id="HEG1168205.1"
                     /translation="MLNITIRGHDLSEVQTIEDLAEKTKEQGIHTLQLALGLSFPEIP
                     SGANEINSGMGNYVKRVLEKQEVTVGILSCYINMIHPDLTIRQELLTKFEQYLRYASS
                     FGASMVASETGCVLPEIQYTEENFTDEAFAEAVSVIRRLVKAGEKYQMMVGIEPGLNH
                     PVYSLARVEQLIQAVDSDYLGIILDPTNLITSSDYQEQVQLVEEAFERFGEKICAVHL
                     KDFRVEQEKIVPVNLGDGVIEYTKIKEIIMKNRPYLYVVLEETKDDGIRYGRSLLE"
     gene            complement(21052..22347)
                     /locus_tag="SCJ86_000620"
     CDS             complement(21052..22347)
                     /locus_tag="SCJ86_000620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288256.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter large permease"
                     /protein_id="HEG1168206.1"
                     /translation="MAIEAGLVLFFVFVVLLVLGMPIAISIAFSSMMTLLLVIPFDVS
                     AFSSAQKMVGSINSFSLIAIPFFVLSGIIMNNGGIAKKLVDFAMLFVGRVPGALAHTN
                     VLGNALFGSISSSAIAASTAIGGVLIPQQVEEGYDRKFATAVNIASAPTGMVIPPSTA
                     FIMYSLVAGGASISALFMGGYLVGALWALGIMLVAFIVAKKNKYPVVEKGQMAHAGKI
                     IKEAIPSLLLIVIIIGGILTGIFTAVEASAIAVIYSLLIAMFYYKTVKLKDIPNMLFQ
                     SVAMSGTIMFLLATSSMMSFAMAFTGIPQAISSLIMGVTDNKFIILLLVNLVLLLVGM
                     FMDVAPAILIFTPIFLPIVTSVGVDPVHYGLFSIMNLCVGSITPPVGTGLYVGASVGG
                     VKAEAMLKPLLPFYGVILVVLFLITYFPQIVMWLPNMLS"
     gene            complement(22363..22842)
                     /locus_tag="SCJ86_000621"
     CDS             complement(22363..22842)
                     /locus_tag="SCJ86_000621"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter small permease"
                     /protein_id="HEG1168207.1"
                     /translation="MKMILNKVLEILGAVLLVGMVAVVLWQVFSRTVLGNPNTVTEEL
                     VRFGLVWFAMLSSAYVVGQKAHLAVTVLSEKLTGKKSKILEVVVQSLFLVFAAMIMVY
                     GGWNAVTLTMGQISPSLSIPMGYVYLSVPVSGVLIIIYSLINLIDTLSNKPAIQEKI"
     gene            complement(22848..23876)
                     /locus_tag="SCJ86_000622"
     CDS             complement(22848..23876)
                     /locus_tag="SCJ86_000622"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter substrate-binding protein"
                     /protein_id="HEG1168208.1"
                     /translation="MKKTSLLFAASSLSLGLLGLSGCGNNSSVATVADADVNEIVTLR
                     FAYASNSQPVIDAMNEFGRLVKEKTDGSVQIQYFPDGQLGGERELIELTQSGAVDFTK
                     VSASALESFSKDYSIFSVPYLFNDEEHFFKVMDNEEIMEPIYQSTKNLGFTGLTYYDS
                     GQRSFYMVDGPIHTPEDLKGKKIRVMQSETAIKMVELLGGSPVPMGSDEVYTSLQSNL
                     INGSENNEFVLYTAGHGGVAKYYSYDEHTRVPDIIIMNDAIKEQLTDEQEKAIEEAAK
                     ESTVFEIEAFAKAIEEEKALAQEEHGVQFNDVDNTPFREAVAPLHDIFKNDPTYKDLY
                     QKIQEQGA"
     gene            complement(23893..24999)
                     /locus_tag="SCJ86_000623"
     CDS             complement(23893..24999)
                     /locus_tag="SCJ86_000623"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629839.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyl hydrolase"
                     /protein_id="HEG1168209.1"
                     /translation="MIIKEEIFVPKELVKEKIDLLVENLTTIKDDNGEFLLDFDGLKV
                     DDKSWTVWNWPQGVGLYGIYKNYQMTKNPRAYQVVNEWFEDRMEEGAPPKNVNTMAPL
                     LTMAYLYEDTKDSRYLPYLEQWAEWVMNDMPRTNEDGLQHATYGPENKNQLWDDTLMM
                     TVLPLAKIGKLLNRPEYLEEARYQFMIHIKYLMDKRTGLWYHGWTFEGNHNYAEAFWA
                     RGNCWLTIAIPEIIEILELSKEDALRKLLIETLEAQVRALKTYQSESGLWHTLLDDPS
                     SYVESSATAGFAYGILKAVHKRYLPQEYKEVAYRAIQGLLEQIDEKGEVQNVSIGTGM
                     GDSLDFYRNIGITAMPYGQSLTVLCLTELLVSYC"
     gene            complement(25228..27825)
                     /gene="adhE"
                     /locus_tag="SCJ86_000624"
                     /gene_synonym="adhC"
     CDS             complement(25228..27825)
                     /gene="adhE"
                     /locus_tag="SCJ86_000624"
                     /gene_synonym="adhC"
                     /EC_number="1.1.1.1"
                     /EC_number="1.2.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294180.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional acetaldehyde-CoA/alcohol
                     dehydrogenase"
                     /protein_id="HEG1168210.1"
                     /translation="MAKVIEKEKTKTIDVQAMIDDLAEKANAALKEMESFDQEKVDHI
                     VHEMAMAALDQHMPLAKMAVEETGRGVYEDKAIKNMYASEYIWHNIKHDKTVGVINED
                     VQKGLIEIAEPVGVVCGVTPTTNPTSTTIFKSMIALKTRNPIVFAFHPSAQKSSAEAA
                     RVVRDAAIAAGAPENCIQWIEHPSIEATSMLMNHPGIAIVLATGGAGMVKSAYSTGKP
                     ALGVGPGNVPAYIEKTAKIKRAVNDLIVSKTFDNGMICASEQAVIVDKEIYAAVKAEF
                     QAHQVYIVKPDELQKLEDAVMNEGKYAVNPSIVGHSAMEIAKLAGISVPKGTKMLIAE
                     LEGVGPDYPLSREKLSPVLAMIKANNTDHAFDLCEGMLNLGGLGHTAVIHSENEELHV
                     KFGLRMKACRILVNTPSAEGGIGDIYNEMIPSLTLGCGSYGKNSVSRNVSAVNLINVK
                     TVAKRRNNMQWFKLPPKIFFEKNSLLYLEKMENVERVMIVCDPGMVQFGYCDTVREVL
                     ARRKNDVKIEVFSDVEPNPSTNTVYAGTKLMADFKPDTVIALGGGSAMDAAKGMWMFY
                     EHPDTSFFGAKQKFLDIRKRTYKIDKPEKTQFVCIPTTSGTGSEVTPFAVITDSETHV
                     KYPLADYALTPDVAIVDPQFVMSVPASVTADTGMDVLTHAIESYVSVMASDYTRGLSL
                     QAIKLVFDYLEKSVKTPDMESREKMHNASTMAGMAFANAFLGICHSVAHKIGGEYGIP
                     HGRTNAILLPHIIRYNAKDPQKHAMFPKYDYFRADTDYADIAKFLGLKGETTEELVEA
                     LATAVYELGKSVGINMSLKAQGVTQETLDTTVDRMAELAYEDQCTTANPKEPLISELK
                     QIIIDAYNG"
     gene            complement(28130..28510)
                     /gene="yajC"
                     /locus_tag="SCJ86_000625"
     CDS             complement(28130..28510)
                     /gene="yajC"
                     /locus_tag="SCJ86_000625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294182.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit YajC"
                     /protein_id="HEG1168211.1"
                     /translation="MGTLPMLVMFIALLAMWFFMSRSQKKQQQERQNLLDSMKPGDSV
                     VTIGGLHGVVSEIDNDKRTVVLDCEGIFLEYDRAAIKTVKPGTVVTNDATTTVETTEE
                     PAETVEVKTETTETLEETEEKEEK"
     gene            complement(28607..29752)
                     /gene="tgt"
                     /locus_tag="SCJ86_000626"
     CDS             complement(28607..29752)
                     /gene="tgt"
                     /locus_tag="SCJ86_000626"
                     /EC_number="2.4.2.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010720296.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA guanosine(34) transglycosylase Tgt"
                     /protein_id="HEG1168212.1"
                     /translation="MTQPAIRYRLIKKEKHTGARLGELITPHGTFPTPMFMPVGTLAT
                     VKTMSPEELKEMGAGVILSNTYHLWLRPGEDLVEEAGGLHKFMNWDQPILTDSGGFQV
                     FSLSDMRNIEEEGVHFKNHLNGSRMFLSPEKAINIQNKLGSDIMMSFDECPPFDESYD
                     YVKKSVERTSRWAERGLKAHANPDRQGLFGIIQGAGFEDLRRQSAKDLVGMDFPGYSI
                     GGLSVGEPKSEMNRVLEFTTPLIPENKPRYLMGVGAADSLIDGVIRGVDMFDCVLPTR
                     IARNGTCMTSQGRLVVKNAKYAHDFRPIDEKCDCYTCKNYTRAYIRHLIKCDETFGIR
                     LTSYHNLYFLLNLMKQVRQAIMDDNLLEFREAFFEEYGFNKENAKSF"
     gene            29970..30668
                     /locus_tag="SCJ86_000627"
     CDS             29970..30668
                     /locus_tag="SCJ86_000627"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293400.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF975 family protein"
                     /protein_id="HEG1168213.1"
                     /translation="MQSSAELKRQAKESLKGRWGQAVLLNLIPTLIGIATILLIMIPI
                     ILFATTIYDGSASVSEATTSGGGSSGGGGGIISSIISALFMSGISWTYLDILRGQKDQ
                     IEPFKDAFRGFKGLFIGGVISLAVLISIFTTLWGLLLVIPGIIKSYSYSQSYFIYYDI
                     VQETGQTPKVLDTITASRKLMDGYKGQLFWLDLSFIGWHILALATAGIGYLWLSPYIS
                     ATKAAFYEQLPKDI"
     gene            complement(30836..33475)
                     /locus_tag="SCJ86_000628"
     CDS             complement(30836..33475)
                     /locus_tag="SCJ86_000628"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298329.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation-translocating P-type ATPase"
                     /protein_id="HEG1168214.1"
                     /translation="MEEYKQTIEEVKKVTEADPDTGLSSQEVDERRKKQGLNKFDEAP
                     KESMIKKFFRSLSDFTTIILLVAAVISFYTAFATEHGDLFEGLLIIAIVVINSVLAIV
                     QEGNAEKALESLQDMNKQTATVIRDGKVTTVESEQLVVGDILVLESGDAISADARLIE
                     ASQLRVEESALTGESEAVEKDAAFIAEEDESLGDQLNMVFKGCTVAAGRGKAIVTAIG
                     MATEMGKIADLLNENTMQKTPLQVRLNQLGKRISMIALAAAALVFVIGELQGEPLLEM
                     FMTSISLAVAAVPETLTVIVTLTLAYGVQKMARKHAIIRQLPAVETLGTANVICSDKT
                     GTLTQNEMRVRRVWSKEDEVTNIEDSMTNSAMEILKMAALCTDVTVEQEDDDLVIKGN
                     PTEVAIVRAVEENYHTKAELEEKYPRVNELPFDSERKMMTTVHQMGEKYISITKGAFD
                     VLAPRFSSGDVEQAAIVNDRFGKRALRVIAVGYATYDEEPQDISSEALEKDLRLIGLI
                     GMIDPPRPESKGAIKRAKKAGIKTVMITGDHVVTASAIAKELGILNDPSEALSGSELH
                     QLSDEELDARVKALSVYARVTPEDKIRIVKSWQRTGAVVAMTGDGVNDAPALKASNVG
                     CAMGITGTDVAQSAADMILTDDNFATIVDAVSQGRSVYQNIRKAINFLLSCNISEIFI
                     VLIAMLLGWGAPFTAVQLLFVNVVADGLPGFALGREPAEHGIMDQPPIPKNEGIFARG
                     LLQKIAINAGVFTIVTLFGYYLGSYVDTISPWVDASQHVGQTVAFLILAYSSILHVFN
                     VRSSQSIFKVNLATNKALVEMALLALAITTAVALLPFTQELFGLVHISLNHWFLVGIL
                     SIVPIAVNELIKFHRLPEAEEEE"
     gene            complement(34094..35125)
                     /gene="queA"
                     /locus_tag="SCJ86_000629"
     CDS             complement(34094..35125)
                     /gene="queA"
                     /locus_tag="SCJ86_000629"
                     /EC_number="2.4.99.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177243.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA preQ1(34) S-adenosylmethionine
                     ribosyltransferase-isomerase QueA"
                     /protein_id="HEG1168215.1"
                     /translation="MLTTEDFDFDLPEELIAQTPLKDRDHSRLLVLDRKTGEMEDKHF
                     HEIIEELQPGDALVMNNTRVLPARLYGEKPDTGAHLEVLLLTNTQGDTWETLIKPAKR
                     AKVGTRISFGDGRLQAVVKEELEHGGRIIDFEYEGIFLEILESLGEMPLPPYIKERLE
                     DPDRYQTVYAKENGSAAAPTAGLHFTKELLDEIEAKGVELVYLTLHVGLGTFRPVSVD
                     KIEEHHMHSEFYRLTEEAAERLNQVRKNGGKIVAVGTTSIRTLETIGTKFNGEIKADS
                     GWTDIFITPGYEFKVVEAFSTNFHLPKSTLVMLVSAFAGRELTLSAYQHAIEERYRFF
                     SFGDAMFVK"
     gene            35318..36493
                     /locus_tag="SCJ86_000630"
     CDS             35318..36493
                     /locus_tag="SCJ86_000630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628506.1"
                     /note="Members of the family are the ATP-binding subunit
                     of ABC transporters for substrates such as betaine,
                     L-proline or other amino acids, choline, carnitine, etc.
                     The substrate specificity is best determined from the
                     substrate-binding subunit, rather than this subunit, as it
                     interacts with the permease subunit and not with substrate
                     directly; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="betaine/proline/choline family ABC transporter
                     ATP-binding protein"
                     /protein_id="HEG1168216.1"
                     /translation="MIEFQNVSKIYKGGKVAVEDVNLSFEKGEFICFIGTSGSGKTTT
                     MRMINRMTDPTQGKILIDGKSIQEIDPVELRRQIGYVIQSIGLMPHMTIRENITLVQK
                     LLKVSQEERNKTAEKMIDLVELPREMLDRYPHELSGGQQQRIGVVRALAANQDIILMD
                     EPFGALDPITRDSLQDLVKDLQERLGKTIVFVTHDMDEAIKLASRIAIMSEGRVIQFD
                     TPENILRHPANDFVEELIGEDRLLQAKPNATRVGEVMLNTAITITPEKSLQEAIKLMR
                     EKRVDTLLVTDNSNVLKGFIDVETLNKKRGKVSSVGDILNKHVFYVKESAYLRDTLQR
                     ILKRGLKYVPVVDEQNKVVGILTRASLVDIVYDVIWGEDPATTDVAESATPEESKEV"
     gene            36497..37138
                     /locus_tag="SCJ86_000631"
     CDS             36497..37138
                     /locus_tag="SCJ86_000631"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325213.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEG1168217.1"
                     /translation="MQQFFHQHGSEILSKGFEHLYISGLALIIGIIIAVPLGVLLTRF
                     PKAATIVIGLTSALQTIPSLALLALMIPLFGVGKLPAIIALFIYSLLPILRNTYIGMK
                     NVDWNYRDVAKGMGMTEFQSIFSVELPIAMPTIMAGIRLAAVYVIAWATLASYIGAGG
                     LGDLIFSGLNNYQPDLILAGTIPVTILALLADWLLGLLEHRLTPIALREENEE"
     gene            37135..38052
                     /locus_tag="SCJ86_000632"
     CDS             37135..38052
                     /locus_tag="SCJ86_000632"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631476.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="osmoprotectant ABC transporter substrate-binding
                     protein"
                     /protein_id="HEG1168218.1"
                     /translation="MKRILKMTMVLFSLLILASCSFPGLASNTDEDTISITGGITSEA
                     QILASLVAGMVEHYTDKNTAIINNLATTTINHQAMMNGDASISAARYTGTDLTTTLNL
                     PPEKDPKKAFATVKDEFEKRYGQTWFPSYSFENTYVFLVRKDTAQKYHLSKVSDLKNV
                     ADELVAGVDTSWINRKGDGYDGFQETYGFSFNSILPMQIGLVYDAVEAGKMDIVLGYS
                     TDGRIASYDLVMLEDDRQFFPPYDAAPVVDNKILADTPHLKEALNKLGHTISTEKMQE
                     LNYEADNNLVEPSVVAQRFLQENHYFEGK"
     gene            38049..38720
                     /locus_tag="SCJ86_000633"
     CDS             38049..38720
                     /locus_tag="SCJ86_000633"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301116.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEG1168219.1"
                     /translation="MKQLDKMNVFQQFFYYFQENGSYIFAQFIRHFLISIYGVLFAAV
                     VGIPVGIMISRRRKLANWVIRLANIIQTIPSLAMISILIIGLGLGVNVVIVTVFLYSL
                     LPIIKNTYTGMIQVDKNILDVGKGMGMTARQRLFMVELPLSVSVIMAGIRNALVVAIG
                     ITAIGAFVGAGGLGDIIIRGTNATEGTSIILAGALPTALMAIITDWLLGILERRLDPA
                     SRTSR"
     gene            complement(39261..39749)
                     /locus_tag="SCJ86_000634"
     CDS             complement(39261..39749)
                     /locus_tag="SCJ86_000634"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307399.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannosyl-glycoprotein
                     endo-beta-N-acetylglucosamidase"
                     /protein_id="HEG1168220.1"
                     /translation="MTRKISKIGASLLVLAVATTIDYRQSEAKTIGNVVYRETAKSML
                     KKAMDNQPESSYWFPEDSLAWSFGKDTDTKYNTSVVPLAERVSKATLPNMNVTQTEEV
                     KVVALSAMNSSINGNASRGIDIFYANVFSYWQYIDQLVYGGALLVKDHRIAKSSCDGC
                     SP"
     gene            complement(39950..40216)
                     /locus_tag="SCJ86_000635"
     CDS             complement(39950..40216)
                     /locus_tag="SCJ86_000635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319468.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Txe/YoeB family addiction module toxin"
                     /protein_id="HEG1168221.1"
                     /translation="MSNYTVAIKNSAKVDLRKIKQTNLKKQFEEVIQTLKEDPYMPTQ
                     SFEKLRPTHEGRYSRRLNRQHRVVYKVDEENKVVEIYSAWTHYE"
     gene            complement(40216..40479)
                     /locus_tag="SCJ86_000636"
     CDS             complement(40216..40479)
                     /locus_tag="SCJ86_000636"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294199.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system Phd/YefM family
                     antitoxin"
                     /protein_id="HEG1168222.1"
                     /translation="MANTTMNPSTARKNFYQLLKEVNENHTEIEIISDRSGNNAVLIG
                     LEDWRAIQETLFLEQTGTLDKVRDREKDDSSFTNIDDIDWEAL"
     gene            40955..41140
                     /locus_tag="SCJ86_000637"
     CDS             40955..41140
                     /locus_tag="SCJ86_000637"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294201.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HEG1168223.1"
                     /translation="MALSYGNKKWSLDEAEIPTYEDTWHLRVEVKEEAENIGGCTLFG
                     KGFGNYDQDIKIKMFYS"
     gene            complement(41282..43105)
                     /locus_tag="SCJ86_000638"
     CDS             complement(41282..43105)
                     /locus_tag="SCJ86_000638"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016922351.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="APC family permease"
                     /protein_id="HEG1168224.1"
                     /translation="MDYLKRLLVGKPLKSAENDEHKLTRFAALALLSSDALSSIAYGT
                     EQIVVVLVALSAAAIWYSLPIAAFVIILLISLTLSYRQIIHAYPHGGGAYVVSSENLG
                     KNAGLISGGSLLIDYMLTVAVSVSAGAEAITSAVPALYGHQVAISVTIVLLLMMLNLR
                     GLRESASFLLFPVYTFILVISLLIVVGLYNIVTGAVPLQATALPGAAVPGVSIALILR
                     AFSSGSSSLTGVEAISNAVPFFKKPRAKNAAATLTMMALILGFFFVGITFINYWYGIV
                     PEKEVTVLSQIGKAVFGHGILYYVLQFATALILAVAANTGFSAFPVLAYNLAKDKFMP
                     HMYQDRGDRLGYSNGIITLALGSIVLLFIFHGSTERLIPLYSIGVFIPFALSQTGMVV
                     KWKKEGKRWLSKSIANITGAFISYAIIAILFVYRLGDIWPFFIIMPIVMFIFYKIHDH
                     YKKVAEQLRLENDAKLHDYDGNTVLVLVGNVTRVNIGALNYARSIGDYVVAMHVSLDE
                     DVEKEKEIEAEFKKHFPDVRFSIVHSSYRSIENPIIRYVDIVSKNAAKQNYTTTVLIP
                     QFVPNRRWQNILHNQTSLRLRLRLSWRENIVVSTYSYHLKK"
     gene            complement(43703..46408)
                     /locus_tag="SCJ86_000639"
     CDS             complement(43703..46408)
                     /locus_tag="SCJ86_000639"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YhgE/Pip domain-containing protein"
                     /protein_id="HEG1168225.1"
                     /translation="MQSIKNTLRLYLLDWKRIFKNPIATLLIIAIMIIPSLYAWFNIK
                     ALWDPYGNTGELPIAVYSADKPAEFQGKEVAIGEQVIESLHKNKQLGWQFVDSKEQLE
                     DGVRSGKYYAGIYLPKDFSEDLLSFTSGDIKKPKIEYTVNEKINAIAPKITDKGASSI
                     QSQITNEFIKTASSTLLKVFNEIGYDIDTNLVSINKVKDMILSTDENLDTIDGYTKQV
                     LELQSQLPEIKEKLNKANEFVDYIPKVDEMGEKVVALNDKMPELKDQAKIILDLQEKI
                     PEIQNAGKQLAEIDGDFASIQETMNQGIDEAKQGLTIIQQVQDILPDVKNLENQASDL
                     ANQTKSGASQLKDAIPSITNSVDVTLKSIGQVATTTGSLIDTVRTAIEDGKLTDDEKK
                     NISNVLNGFIDGISQQQNAIDKLVEFLTKLQTSAGNTKLQPIIDQLNQLKPLLSNLQN
                     RLSALNTAVQNEDTKAIDELLDQIDAVVNNINGIIAGINTGQISSTVTSLLDQVIDTI
                     GTAQGALSKADQIDFDSLLSSTKATVTNAVAILEKYQKEMPAIGQEIHDANTMLNGNM
                     DTIVNGINEGADLYNNELPIIQQKLGLAADFIQNDWPALKNEITGTLTTVNEKMPEVE
                     TAVNAAADLINNDWPNIKAGIQKAADAIRKGEQEVDLGEVIKLLKLDATKESDFFTQP
                     VELQTNALYPIANNGSASTPFYTALCLWVGAVLLSSVATTEYYLGKKDRNNFTKREQF
                     VARMLTFLTMAIAQSLIVTLGNIFLLGVDVQNPVYSVSFAVLIALAFMMIVYVLAALF
                     DNVGKGIAIIILVLSISGGGGNYPIQVSGKFFQMINPFLPFTHAVNLLRESAGGIYWP
                     NATKAIWIMIGLFVVFGIVGTAIYPFIEEKMTKFKEAAHESHIFH"
     gene            complement(46714..47661)
                     /locus_tag="SCJ86_000640"
     CDS             complement(46714..47661)
                     /locus_tag="SCJ86_000640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002352127.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-dependent oxidoreductase"
                     /protein_id="HEG1168226.1"
                     /translation="MRRFAINGYGEAQDVFEEIDAHPREVGSGHVRVEIKAFSVNPYD
                     VALRLGEMKEIRTLKFPYVPGNDGAGIVTEIGSDVTTVHVGDRVAVHAVGGTYGEEVV
                     LPSAKVAKIPDKMSWEEAAGMVTPGITAYNLINHLTEIQPTDTVMILGASGAVGSSLI
                     QLLHEKGIRILTSASSKNEEKVKKLGASAFAAYDKTNPGLQFADQADLVIDATKGSIK
                     GETGIQIMKPGGRYVALNDLPDLDLRQKKEGFYESFVPRKEYLDAEAFAGIIKAYQKG
                     AFHVFISMNLSASLKHVIQAHQLVEGHPPAGKIILSFEK"
     gene            47851..48120
                     /gene="rpsN"
                     /locus_tag="SCJ86_000641"
     CDS             47851..48120
                     /gene="rpsN"
                     /locus_tag="SCJ86_000641"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010750653.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S14"
                     /protein_id="HEG1168227.1"
                     /translation="MAKKSKIAKAKKQQETIERYAALRQELKAQKDYEGLRKLPLDAR
                     PERLKNRDLIDGRPRGYMRKFGMSRIKFRELAHKGLIPGVKKASW"
     gene            complement(48296..48658)
                     /locus_tag="SCJ86_000642"
     CDS             complement(48296..48658)
                     /locus_tag="SCJ86_000642"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system PemK/MazF family
                     toxin"
                     /protein_id="HEG1168228.1"
                     /translation="MVKRGDIYFADLSPVVGSEQGGTRPVLVIQNNLGNHFSPTIIVA
                     AITAKMAKPKLPTHIGIKATGTGIERDSVILLEQIRTIDKSRLKEKVCHLDRPIMAEV
                     DRALKISVGIEALTPVTK"
     gene            complement(48669..49790)
                     /locus_tag="SCJ86_000643"
     CDS             complement(48669..49790)
                     /locus_tag="SCJ86_000643"
                     /EC_number="5.1.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296555.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alanine racemase"
                     /protein_id="HEG1168229.1"
                     /translation="MVVAWHRPTKAVIHKKAITENVANEVARLPQGKELFAVVKANGY
                     GHGAIETAEAAVAGGASGFCVSNLDEGVELREAGFTQPILILNMVPYDALTVAVAHDL
                     SVTAGTREWLQAAAAVLEKSKLETPLSIHLKADTGMGRIGFCTPEEVKEAAAFIKESR
                     VLEWEGLFTHFSTADQADDTYWNLQKERFIEVLKKLPELPRYVHVSNSATALWHDETI
                     GNMIRYGVAMYGLNPSGHALPEVYPLQPALELVSELIQVKKLPAGEGIGYGETYITPE
                     AEWIGTIPIGYADGWPRKMQGFSLLVEGNYCETIGRVCMDQLMIRLPQEFPVGTKVTL
                     IGKNADKEITMQDIADQLGTIHYEVACGLGQRIPREYQE"
     gene            complement(49806..50156)
                     /locus_tag="SCJ86_000644"
     CDS             complement(49806..50156)
                     /locus_tag="SCJ86_000644"
                     /EC_number="2.7.8.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293418.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="holo-ACP synthase"
                     /protein_id="HEG1168230.1"
                     /translation="MIKGIGIDAVELPRITRLIEEKPKFIARILTSDEMKLFQSLPFH
                     RQVEFLGGRYACKEAFSKAWGTGIGKVTFQDVEILKNENGQPVVTRSPHEGNVWVSIT
                     HTNETAFAQIILED"
     gene            complement(50286..51797)
                     /locus_tag="SCJ86_000645"
     CDS             complement(50286..51797)
                     /locus_tag="SCJ86_000645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623717.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HEG1168231.1"
                     /translation="MKFKELELSPELLKSVERAGFEEATPIQAETIPLALAGKDVIGQ
                     AQTGTGKTAAFGLPMLEKIDPDRHELQGLVIAPTRELAIQTQEELYRLGRDKKVRVQA
                     VYGGADIGRQIRGLKDRPHIVVGTPGRMLDHINRHTLKLGTVQTLVLDEADEMLNMGF
                     LEDIEKIISQVPDQRQTLLFSATMPPAIKNIGVKFMKNPHHVKIKAKEMTADLIDQYY
                     VRAKEYEKFDIMTRLFDVQTPELTIVFGRTKRRVDELARGLEARGYRAEGIHGDLSQQ
                     KRMSVLRSFKSGHLDILVATDVAARGLDISGVTHVYNYDIPQDPESYVHRIGRTGRAG
                     KGGMSVTFVTPNEMDYLHVIENLTKKRMTTLRPPTEKEAFKGQLGAAVEQIETKLAEN
                     GLDKYLQTADKLLEEYSAQDLVALLLKTTAKDPADAVPVKITPERPLPMQKKGYNKNG
                     KRGGGNNRNRRDGGNYRGNKSKGGYSKNNNSQKDGGKRHNDKKRGFVIRSNND"
     gene            complement(52119..53471)
                     /locus_tag="SCJ86_000646"
     CDS             complement(52119..53471)
                     /locus_tag="SCJ86_000646"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002354419.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-tripeptide--D-alanyl-D-
                     alanine ligase"
                     /protein_id="HEG1168232.1"
                     /translation="MNLTIQEIAQAVGSECSALTKITNVEFDSRKITEGSLFVPLAGT
                     RDGHEFIQQAITNGASASFWSLPVEQAPKNFPVIPVADPLKAMQQLATYYLEKVGAEV
                     VAITGSNGKTTTKDLTASVLSEKYKTYKTQGNYNNNIGLPYTILHMPQDTEKIVLEMG
                     MDHANEITELSLMAQPKAAAITMIGEAHIENLGSREGIAKAKMEITDGLVSDGLLVVP
                     ADEPLLTPLTATLVQTVTTFGIGNGDVHAEVTAKGKAQTDFVVEGETFTIPLPGSYNV
                     TNALIAYTIGRFFGLSIPEIRQGLAHVSITQNRTEWLTAGNGAAILSDVYNANPTAMG
                     LVLDTFKNLPTKGRRIAVLADMLELGKESLNMHAQMSEHIDPEKFAMIFLYGEQMHAL
                     KEELNQKYPQLPVFYFEKEKNKLIDAIKQNLEATDSIMLKGSNSMGLFEVVEQLQQMK
                     "
     gene            complement(53624..54700)
                     /locus_tag="SCJ86_000647"
     CDS             complement(53624..54700)
                     /locus_tag="SCJ86_000647"
                     /EC_number="6.3.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736859.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanine--D-alanine ligase"
                     /protein_id="HEG1168233.1"
                     /translation="MKITLLYGGRSAEHEVSILSAFSVLNAIYYNYYQVQLVFITKEG
                     QWVKGPLLTEKPASKDVLHLSWDPSGQTEEGFTGKVINPGEIKEEGAIVFPVLHGPNG
                     EDGTIQGFLETLNMPYVGAGVLTSACAMDKIMTKYILQAAGVPQVPYVPVLKNQWKEN
                     PKKVFDQCEGSLLYPMFVKPANMGSSVGITKAENREELQNALATAYQYDSRAIVEQGI
                     EAREIEVAVLGNEDVRTTLPGEVVKDVAFYDYEAKYINNKIEMQIPAEVPEEVYQKAQ
                     EYAKLAYTMLGGSGLSRCDFFLTNKNELFLNELNSMPGFTEFSMYPLLWENMGLKYGD
                     LIEELIQLGMNRYHQRQSFFEKNE"
     gene            54896..56641
                     /locus_tag="SCJ86_000648"
     CDS             54896..56641
                     /locus_tag="SCJ86_000648"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342755.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AarF/ABC1/UbiB kinase family protein"
                     /protein_id="HEG1168234.1"
                     /translation="MAQTNPRSRLAEIIRVFINYNVVPNFVQQKNPEQVKKAFEELGP
                     TFIKIGQMLSVREDLLSSAFTQTFKTLQDSVPSDTFSTVKKTIETELSLSLSDIFDDF
                     SKSPFASASMGQAHRAKLKNGDAVVVKIQHPNIAEEIRLDLQLFERAIPLIKYIPETS
                     VVDLKGVLQEVKRSLINEMDFLKESQNGEQFYQKNNGWKEIRSPKIYDAFCSKKVIVM
                     EEMSGKNLNHLMNAENKTETFITGIQNKQLKQEVAKLLVENFMKQVFDDGFFHADPHP
                     GNLLFHVLTKEEQTQASRKTETVHEKEFGSFAFRASTSAEDPVAPYTINYIDFGMMGH
                     LSAGLRQKLTQAVLALYTKDAYRIEKAVLRLCQQEGSFDESRFHQELTSFLEQYYDSP
                     IDEINLQEVFTHVVTICHQNNLQFDRDITLLLKAFGTLEGVIRVLDPEVSLMEVASPF
                     AQHYFLTHLDVEDTLKQSGLDLLEGMKAAPKIPQQLHHLLEMWTSGQGKVNLELKKQD
                     KLLSRIESMINRLVFGMILAALIVGSSLLVQAAPVENAEVVSLLGIFTYAIAAFVIIF
                     LAIDALIQMYKKRKK"
     gene            complement(56921..57358)
                     /locus_tag="SCJ86_000649"
     CDS             complement(56921..57358)
                     /locus_tag="SCJ86_000649"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296550.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YaiI/YqxD family protein"
                     /protein_id="HEG1168235.1"
                     /translation="MRLFIDGDGSPVKEDVIKLGESYGLDVVIVTSVDHYTTKEYANH
                     VQIVYVDRGADSADYKIVGMVKKNDFLITQDYGLASLVLPKGVRVFHHSGKEFDEANI
                     GTLLEQRYHSGKLRKAGIRTKGPKPFTEKDHMSFRQALKKAID"
     gene            complement(57342..58715)
                     /locus_tag="SCJ86_000650"
     CDS             complement(57342..58715)
                     /locus_tag="SCJ86_000650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEG1168236.1"
                     /translation="MFEKLKRFGRNFTQTTPFILSNTVIFIPYLLFLGLTDSHNWLTI
                     LPFTLFYAFRMTGLFLVNSLRFGLDSYTLLMIALLIGGAGSLLGILGVFYFPLFYFSS
                     VLLGLSAAWLVPANTTVNFHEKTQGFINMDGKKYLFAAIWLFLLYQAIELPAPTQIAA
                     SFTIYTLFYVMAYHTVSHYPRYELDFKDIKRNLVATRELVLFLIFFGLLFLLRNARLL
                     FDERLFDLAIYGFLLLFILFVFVLGHQKKEWKLPSWLNLFTFLNGMLGNFLFLFSSFY
                     VGILYGLEKLSIFMYLPYAAGLIAAKIVGPWILKRLTWSPLAMQLLGLSSSLLVLLIP
                     NGFAIGVFLLSFWHVVTGSWLNKEYYQTDTAIPMDRRIVVKYTTQNKGSVIHQFLLMT
                     LLFVLAKEMGEPIHLLLITLNHESVPIESVQLLILAKWINVGLLLSGLVTVYHLWKKA
                     KKNEAIH"
     gene            complement(59037..59525)
                     /locus_tag="SCJ86_000651"
     CDS             complement(59037..59525)
                     /locus_tag="SCJ86_000651"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEG1168237.1"
                     /translation="MEKIKVTENELDELMAVIQEVWPEVFVPIIGQKQVDYMLKTYQS
                     KKQIQKELTEGVSYFLLKSEEKTVGYTAYEEINGKIYLSKLYLLNKVRGQGLTSSVFR
                     WYEELAAQTKEREIFLRVNQGNHQAIEVYKHEGFEITEELISDIGQGFQMVDYKMKKA
                     LA"
     gene            complement(59547..60149)
                     /locus_tag="SCJ86_000652"
     CDS             complement(59547..60149)
                     /locus_tag="SCJ86_000652"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TVP38/TMEM64 family protein"
                     /protein_id="HEG1168238.1"
                     /translation="MSKKGSVLQKCLQIMPIIGLFFFFVLILYAYHHGIFRSSTSLQA
                     FIQQFGKYAVMIFVLLQVIQVIIPILPGGISSVAGMLMFGNGFGLLYSCIGLVIGEAI
                     GFLLVRYYGVAFVQLILSPKKYQKFDELLTRKTKDIKKVLVVTLLLPFAPDDLICLVA
                     GLTKLSFREFMQIVIFLKPWSVGVYSMIMLFLFHQAQGHL"
BASE COUNT        17104 a        13016 c        10989 g        19084 t
ORIGIN      
        1 tattttagac ttatacgtta ttgtcctgtt tcatttgatg gaggattgta aataaaaaaa
       61 gaggaaagaa cataactttt cgttatcttc tttcctcttt ataattgttt agcttattta
      121 gccaaagctt cttttgcttg gtcagccaat gcagtaaatg ctgctgcatc gttaacagcc
      181 aagtcagcta acattttacg gttgatgtcg atttcagcaa ctttcaaacc gtgcattaat
      241 tttgagtagc tcaagccatt catacgagct gctgcgttga tacgagcaat ccacaattta
      301 cggaagtcgc gtttcttttg acgacgatcg cgatatgcgt agttgtatga attcattact
      361 tgttcttttg ctgttttaaa taaagtgtgt tttgaaccgt agtaaccttt tgctaatttt
      421 agcactcttt tacgacgttt acgagttact gttccacctt taacacgtgc catgtataat
      481 tcctcctaat aaaaataaat cttcagattc tgttatttgt acttcttatt tcatttttgc
      541 tagttgttgg cgaatacgtt tgaagtcgcc actagaaacc atgcttgctt tacgcaattg
      601 acggcgttgt ttttttgttt tgccgtggaa acggtgactt gtaaacgcgc ggaagcgttt
      661 caatccgcct ttaccagtac gtttgaaacg ttttgctgat ccgcggtgtg ttttttgttt
      721 tggcatgact atatttcctc ctcaaaatct ttcgttatcc gactatcggc tgtccaacag
      781 ccagttagtc attcaaatta cttactgtcg tttttcggtg ccagcgtcaa gaacatgctg
      841 cgtccgtcca ttttcgcttt ttgttccact gttgcaatat ccgcagtttc ttcagctaag
      901 cgattcaaga ctttttgacc aatttcttta tgggtaatgg cacggccttt gaaacggata
      961 gaagctttca ctttatcgcc cttttccaag aacttacgtg cattacgaag ttttgtattg
     1021 aagtcattca agtcgatcgt aggacttaag cgaacttctt ttacattgat cactttttgt
     1081 tttttacgcg cttcgcggtc tttcttttgt tgttcgaaac ggtatttacc gtagtccatg
     1141 attcgcgcaa ctggtggttt cgcaccaggg gcaactaaca caagatccaa gtttgcttgt
     1201 tctgcaatat tcaatgcttc tgcttttgtc ttaactccta attgctcacc atctgaaccg
     1261 atcaatcgta actctcgtgc acgaatgccg tcgttaacca tcatatcctt tgctatggtc
     1321 attcacctcc aaatattatg agagaaagaa aagcttaccg aataaacggc aataaaaaaa
     1381 cgagttctct tacagaaccc gcgcgtactt cacctaatag aaccagtgaa aactatctag
     1441 cccagtgaca ttatcaacta aggcgagaag cgggtgcttc tgcttttatt tctcaaccat
     1501 catagtatag caaaaactaa tctttttgtc aatgtttttc atacagtttg tcttcctttt
     1561 ctgaaaatga ttagattagc agactttcta gcagtgattc ccgcttcttt gctatactaa
     1621 agaaaaacga ggtgaaccca catgaaaaaa atcgaaccta ttccaccttc tcttcctctt
     1681 cttacaaagg gagagtttta tttagaagat gaggagatct tctctggtct tctgatcaac
     1741 gggggtgacc aaagctatct cgatgccaaa aatatggtat taagagagag tcatatccaa
     1801 aaacttacga tgcaaaaaac agttttacgt cattttgaat gcagtaatgt catttttgaa
     1861 aaatgtgatt tttccaatct ggagtggatt ggtggcagtt tccaccaagt cgtgtttcat
     1921 caatgtaaat taacaggtac gaattttgcc gaaagttatc ttcgagactg tactttcact
     1981 gattgtattg cttcgatggc ttcctttagt agtacaaact tgaaagctgt ctccttcgat
     2041 cattgtcaat tagaagacag cgaattttat gaggtcactt ggaaaaacct ttttttgtct
     2101 gacaatcagt tgaccggttc gaactggttt cgaacttctc tgaagggatt agactttacg
     2161 actaatttct tcacagcaat cgctttatct caagattctt tggcgggact aatcgtcaac
     2221 caagagcaag cacttgtgat cgcccaagcc cttggtttgc agatcaaaga ctagatcaac
     2281 gaatccgccg ccacagtgca ataagcatcc aaatagataa gaccaaactg atgatcaagg
     2341 cgattcccca tccgaaaacg gaatgggtaa agggtaactg gagattcatt ccgaagaatc
     2401 ctgtcacaat agttggaacg gtcaagatca gcgaccagac cgtcaaaaat ttcatcgtgt
     2461 cattcaagtt gttattcagc aaattattat aagtgccaga gagctggtct aaaatctgcg
     2521 aagctaagtt cgtcatttca actgcttgtc gtgcttcgat cagcgcatcg tccaactctt
     2581 ctttttcctt ttctgacaac ttgcgataaa tagccaatgc cttgatttgt tctaaaagta
     2641 ctacattttg ttttgttcct gtaactaagt aaaccaaacc gatctctaaa tctgaaagtt
     2701 gcagcagatt cttgttcgtt gttttttctc ttaatttcag attaagccgc tgacgttcac
     2761 tattcacttc ctcgattaaa ggaaaaaagg aatcagaaat caaaaacaat gtacgaaaca
     2821 ataaactgta tacactcatg tctggcattc gttccaaaag cgatcgaatt agtcgaatca
     2881 cgtactcggt attcggcgtg gtaaatgtaa acaaaccgtc ttcttttaaa ataaaagcga
     2941 taggactagt ttcataatga ttatcgactt tttgctggtg cggaacattt gatacaagca
     3001 gtaacgcttc atcaaaagaa tcgtactcta cgcgcgcccg ttcattttta tctagagaat
     3061 aagtcagcat ctcgtcacta atgccatatt cttcttttaa gtttttaaca gctagcacat
     3121 cgttttgctg tacattgatc caaaaattac ggtcttgatt aaaatagtgt ttttcccgca
     3181 tactctatcc tcctgacatt tgtttccagt ataaagaaaa aagtcgctca aataaaggtt
     3241 taccctttat ttgagcaact gtaaatcaaa tggctacttt ggtttggcga atctctgcta
     3301 atttttcttc gatcattttc aagacttctt cttttgcttg aggatcttcc acaaaattat
     3361 agcggtctcc atcgatttgg atttttggac tttctttgta ttcttcatac cattgatcgt
     3421 aacgtctgtt caattctttg taataatcgt atagacttgg atcaaaggac aattgttcat
     3481 acgaacgacc acgtttttcg atacgctcca acatcgtatc aaaagataca cgaatatgaa
     3541 ctaataaatc cggatgtttt ttatgggcag catagggaag ttcttccatc atgttttcca
     3601 gcagttcatc atacacacga acttctgttt ctgttgctct tcctagatct gcatttagat
     3661 ggaaaagcag tgaatcttca taaatcgaac ggtccaatac attattgtca tctttcattg
     3721 cttgtttgat gcttgcaaaa cgtttattca aaaaataaat ttgcagcaaa aaagcatact
     3781 gttcagggtt tgcataaaat aagggcaaga cttcattatc atcaatcgat tcataaaatg
     3841 cctgactatc aaagtgttca gccatcattt ctgttaaact tgattttccg gcaccaattg
     3901 tacctgctaa aacgatcacg ctcatttacc tccagctcac ttcatcacta aatattgtgt
     3961 ttaactccaa cctttttaaa tataccccca atatctagta gtgtcaattg tatttttttc
     4021 tagtcaaaga agaaaaaaag tcaaaaaatt cttaagaaaa aagtgttttt tctttagaaa
     4081 acgtattctt agttttactt ctgagatggt tttagtaaaa taaaggagga atgttttttg
     4141 agacccttag tggacaaaaa aattcgtgaa gcagcgcaac tcgttttggg cgctttattt
     4201 tttgcttaaa aatcactttt acgtgagaag gagttagatt atggcaaact atggccaagg
     4261 agaatcaagc ggaaagatca tattgatggg cgagcacgcg gttgtttatg gagaaccagc
     4321 gattgccttt cctttctatg caacaaaagt caccgcattc cttgaagagc tggatgcaat
     4381 ggacgatcaa ctggtttctt cctactattc aggaaattta gccgaagctc ctcatgcatt
     4441 aaaaaatatc aaaaaattat tcattcactt aaaaaaacag catgacatcc aaaaaaactt
     4501 gcaactgacc attgaaagca cgattcctgc tgaacgtgga atgggatcaa gcgctgcagt
     4561 cgccacagca gtcactcgtg ctttttatga ttacttagca tttcctttgt ctcgtgaaat
     4621 actattagaa aatgtccagc tttcggaaaa aatcgcccac ggtaatccta gtggaatcga
     4681 tgcagccgct actagcagct tgcagccgat ttattttaca aaagggcatc ctttcgacta
     4741 cttttctttg aacatcgatg cttttttgat tgtcgctgat acaggaatca aaggacaaac
     4801 aagagaagcc gtcaaagatg tcgctcacct ctttgaaacc cagcctcatg aaactggaca
     4861 aatgatccaa aaattaggat acttgacgaa gcaagcaaaa caagcaatca ttgaaaattc
     4921 accagaaacg ttggcacaga caatggatga atcacaatca cttctggaaa agctgacaat
     4981 cagcaatgat tttcttaatc tattgatcca aacagcaaaa gataccgggg cattgggtgc
     5041 caaattaact ggcggtggac gcggtggctg tatgattgca ttagcacaaa caaaaacaaa
     5101 agcccaagaa atcagtcaag cacttgaaga tgcgggtgct gctgaaactt ggatacaagg
     5161 attaggagta catacctatg tttaaaggca aagcacgcgc atataccaac attgctctaa
     5221 tcaaatattg gggtaagaaa aatgaagaac tcatccttcc aatgaacaac agcctttcgt
     5281 taacattgga tgcattttat acagagacag aagtcatctt ttctgatagc tatatggtag
     5341 atgaatttta tctagatggc accttgcaag acgaaaaagc aacaaaaaaa gtcagtcagt
     5401 ttcttgacct ttttcgtaaa gaagccggcc tctcactaaa agcttcagta atcagtcaga
     5461 atttcgttcc taccgcagct ggattagcct cctctgccag cgggctagct gctttagcag
     5521 gcgcttgcaa tactgctctt aagcttggat tagatgatct ctctctttca agatttgctc
     5581 gacgcgggtc tggttcagct tgccgaagta ttttcggtgg tttcgtcgaa tgggaaaaag
     5641 gccatgacga cttaagttct tacgctaagc cagtcccttc cgattctttc gaagacgatt
     5701 tagcaatggt tttcgttttg atcaacgacc agaaaaaaga agtgtccagc agaaatggga
     5761 tgcgtcggac agtcgaaaca tccagttttt atcaaggctg gttagattcc gttgaagggg
     5821 atctatatca attgaaacaa gcaatcaaaa caaaagattt ccaacttctc ggagaaacga
     5881 tggaaagaaa cggactaaaa atgcatggaa cgactttagc tgcccagcct ccattcactt
     5941 actggtctcc tgacagctta aaagcgatgg atgcggtgcg acaattgaga aagcaaggca
     6001 ttccatgcta ctttacgatg gatgctggcc ctaatgtcaa agttttagtg gaaaacagcc
     6061 atttatctga ggtacaagaa acatttacca aattatttag caaagaacaa gtaattactg
     6121 cacatgctgg tccaggaatt gcgattattg aataaaggga gatttttatg attgaagtat
     6181 ctgcaccagg caaactctat attgccggag aatatgcagt tgttgaaaca ggccatccag
     6241 cagttatcgc tgcagtcgat caattcgtga cagtcactgt agaatctgca cgaaaagtcg
     6301 gaagtatcca atctgcacaa tatagtggga tgcctgtacg ttggacaaga cgcaacggag
     6361 aattagtttt agatattcgg gaaaatcctt ttcattatat ccttgctgct attcgcttga
     6421 ctgaaaagta tgcacaagaa aaaaacattc ttttatcctt ttatgatctg aaagtaacaa
     6481 gtgagttaga cagttcaaat ggccggaaat atggattggg ttctagcggt gccgtgaccg
     6541 ttgcaacggt caaagccttg aatgtttttt acgcgttgaa tttatctcag ttggagattt
     6601 tcaagattgc agcactagcc aatttagcag ttcaagataa tggttcctgc ggcgacatcg
     6661 ctgctagctg ttatggtggc tggatcgctt tctcaacctt cgaccatcct tggctccaag
     6721 aacaagaaac tcagcattct atcagtgagt tacttgccct ggattggcca ggtctatcca
     6781 ttgagccatt gattgctcct gaagatttac gtttattgat tggttggacg ggtagccctg
     6841 cctctacttc tgatttggtc gatcaagttc accgttcgag agaagataaa atggtggctt
     6901 atcagctttt cttaaaaaat agtacagaat gtgtcaacga aatgatcaaa gggtttaaag
     6961 aaaataatgt aacgttgatc caacagatga ttcgaaaaaa ccgacaatta ctgcatgatt
     7021 tatctgcaat cactggggtc gtcatcgaaa cgcctgcttt gaacaaattg tgtaatttag
     7081 ctgaacagta tgaaggagcc gcaaaatctt ctggtgcagg tgggggcgat tgcggaatcg
     7141 taattgttga ccagaaatct ggcattcttc ctttaatgag tgcatgggaa aaagcagaaa
     7201 tcactccact gccgttacat gtctatagcg atcaaagaaa ggaaaaccga tgaatcgaaa
     7261 agacgaacat gtttcactag ctaaagcctt tcatgataaa caaaaaaacg aatttgactt
     7321 cgtacggatc atccataacc ctttgccgca aatagcagtt tctgatgtcg atctaagtac
     7381 acaggcagtt gggtttacac taagcagccc attttatatc aatgcgatga ctggcggaag
     7441 cgaaaaaaca aaaaaaatca accaagatct tgcaatcgtc gcaagagaag cagatttgat
     7501 gattgccact ggttcagtca gcgctgcctt gaaagtccct tctttagccg atacttatac
     7561 gattatgcga caagaatatc ctcacgggaa aatcattgcc aacatcggtg cagggacttc
     7621 tgtcgaaaga gccaaagaag ctatccgatt gtttcacgca gatgccttac agattcattt
     7681 gaatgcacct caagagttgg ttatgcctga gggagaccgt gattttacca attggaaagt
     7741 acttattcaa gaaactcaaa cagccattga cgtccccctc atcgtaaaag aagtcggttt
     7801 tggcatgaca agagaaacgc tcaatgatct agctgcttta ggagttcata cagttgatat
     7861 cagcggtcga agcggaacaa gcttcaccca gatcgaaaac gcccgtcgct ccaagcggga
     7921 actgagttat ttagctgact gggggcaatc gacagtctct tcattgcttg aagcaaatga
     7981 agcagacact tctatggaaa tcctagcctc cggaggtatc cgcaatgcct atgacatatt
     8041 taaagccctt tgtctcggcg caaatgccgt gggtacttca ggaaccgtac tgacgcatct
     8101 gatgaatcac ggcgtagaag aaactattat actgatgaag caatggcaag aagagcttcg
     8161 tctgctttat actatggtcg gcgcaacgaa taccgcggcc ctacaccaac aatctctgat
     8221 cttttccgga ccagtgaagg attggtgcga agcaagaggg atcgacttag tgaagtatgg
     8281 aagacgcaca gaaaagagcg tgggacaaaa gtaaagaaca cttttgtctc acgctctaaa
     8341 cacgtacaaa cggcggaagt aaaagcaact ccttcggaaa taagctgaaa ttcacaaaaa
     8401 tttgaaaagc aattttcgtg aattccttct tatttcttgg agttaaatgc ttctgtccca
     8461 gcctcttttt tcgaataaat gatgtttaat cagctgtcct tcggtattcg ttcaatccgc
     8521 tgatttcaca acctctccac taaacagtgt actaaagctc ttttaaggtt gttttccgat
     8581 ataggcaagt atgcctccgt ccacatacaa gacatggccg ttaacgaaat cagatgcgct
     8641 agaagccaag aagacagatg gtccagccaa atcgattggc tcgccccagc gtgctgctgg
     8701 cgtacgtcca acgataaatt gatcgaatgg atgacgttca ccgttttctt gtgtttcgcg
     8761 taatggagca gtttgcggtg tagcaatata acctggtcca attccattac attggatatt
     8821 gtattgaccg tattcagaag cgatgttttt cgtcagcatt tttaatccgc cttttgccgc
     8881 agcataagca ctgaccgttt cgcgtcctaa ttcactcatc atggagcaga tattgatgat
     8941 tttaccgccg cctttttcga tcatatccgg aataactgct tttgaaacga tgaatggggc
     9001 atttaaatcc acgtcgatta cttggcggaa gtctgccgca tccatttcga tcatcggtgt
     9061 ccgtttgatg atccctgcat tgttgactag aatatcaact gaacctacat cttctttgat
     9121 ctgttgaacc atcgcttgaa ccgcttcttc atcggtcaca tcgcagacat aaccttttgc
     9181 atcgatgcct gcttctttat aatgcgctat accttcatca actgattctt gattcaagtt
     9241 attaaatacg atttttgctc ctgctgtagc taaagagcgt gcaatctcaa atccaatacc
     9301 atagacagca ccggtgacta atgctacttt tccatctaaa cgaaacatat ccatactaaa
     9361 ttccatcatt ttctctccct tatgcttatt ttaattgatc catttgcacc atatccatgt
     9421 ctgtataggt gatattttct ccgcacattg cccagatgaa tgagtaattg cttgtaccta
     9481 cacctgaatg gatcgaccag cttggcgaaa taatggcttg ttcattgtct acaactaaat
     9541 gttttgtttc atctggtttg cccatcatgt ggaacacacg tgtatcttca ttggcataat
     9601 caaagtagac ataagcttcc atacgacgtt catgtgtatg acaaggcatc gtattccaag
     9661 cgcttcctgg ttctaaaatc gtataaccca tttgtaattg acagctttcg catacatttg
     9721 gatggatgta ttggtagatt ttacgttcat tcagtgtcag gccttcacca gtctccattg
     9781 gttttacttg gtcgatactg atttttacat ttggatattt atgatgagct ggaacagaac
     9841 taatatagaa tttagctggg ttttcagcat cttgagaaga aaatacaaca tgtcgtgttt
     9901 cttttccgat atagtatccg tcttgttttt tcatttcttc tttttgccca tcgatctcga
     9961 tgaatcctgg tccaccgata ttgatcacac ctagttcacg acgctctaag aagtaatcaa
    10021 cacctagttc tttgtctaat gtgatttcta attctttttc agtaggtgtc actccaccaa
    10081 agatcattcg atcgttgtgc gtataagtca aactgatttt ccctggttca aatacctttt
    10141 ctactaaaaa ttcttttctc aattgttccg ttgaataatg acggatatct tctggactgt
    10201 gtgtataacg tgtttccatt tcttgcattt tcttctactc cttctatcga atgatttttc
    10261 cagagccgca tgctaaaaat tgtgcgactt cttcttgact aaattggttg cagtccccat
    10321 ggatcgtatg cttcaaggca gaagctgccg tagcaaaatc aatgatttct tgcggagaca
    10381 tattggataa aatcccatgg agcacgccac ctgcaaaagc atctccgcca cctactcgat
    10441 ccacgattgg ggtgattcta tggacttggg attccacata ttcatggttc atccacaatg
    10501 tccccttcaa ttcattttca cttgctgaat gaacggtacg cttggtcgaa taaaacacct
    10561 gaatattcgg gaacttttcc tgcatttttt gataataata aattagaggt tgttcttctg
    10621 cttctggcgc tttctcgata cctaacaaat aaagagcatc catttttcca gctgagcaga
    10681 tatctactaa aggtaaaagc tgatggatta cttcgctggc ttcttgctgt gtccataatt
    10741 ttccacgata gttgatatcg aagctgatca ggcagccagc tttttttgca gcttctatca
    10801 cctgtttcgt cattttttgc cattttttag acaaagctgg ggtgattcct gaaacatgaa
    10861 atatatctat tccttggaat aactcctcta gcgaccattc caaatgatcc atttcagcaa
    10921 aacttgatcc ggcacgatca tagatcactg aggcggcgcg ttcgccacta ccagtttcca
    10981 tataataagt acctagtctt gatccaccag ataatacctg tttcgtatct actccataac
    11041 attgcaagtg ctttttgact gcttttccca atgcgttatc aggtactttt gtagcaaaaa
    11101 tcgcttcatg tccgtaatta gctaaagaaa ttgctacgtt tgcttcccct ccgccataat
    11161 gggcagcgaa tcgttcactt gaatcgatac gggttccctg ctctgtggac aatcgcaaca
    11221 tgatttctcc gagtgtcact acttttccca taagcagcta cctcttgatt tctttcattt
    11281 ttgctgcata ctgttgcgcg acttccgtta ctttgtcaaa atctcctgta gctgcgggtg
    11341 ctaaaaggtt cccacctact ccgactgtta cgacaccagc atcgaaccac tctttcatat
    11401 tgtccagact cacaccgcca gttggcatga tattgacttg aggcatcggc gccttgaagg
    11461 cagaaatgat acttggtcca taagcgcttc caggaaatag tttgacgata tctaccccgc
    11521 ttttcagcgc tgttttgatc tcggtaatag tcatacatcc tggtaagtaa ggaatttgat
    11581 acagattgca catctctgcc gtttcttgat caaaggaagg gctaacaata tactctgcgc
    11641 cagctaagat agccaagcga gctgtgacag cgtctaatac tgtgcctgcg ccaatcacta
    11701 tttctggttg gtctttgtag aatgacaaaa gttctttgat tacttgatct gcttgaggga
    11761 ctgtaaaagt caactcaata ccagttagac cacctttgat gattgcgtgg caagcattca
    11821 aagcttcttc tttgcttttt ccgcgtacca ccgcaatcac tccggcgttt ttcaatcgag
    11881 ataaaatatc aatttttttc atcttctcca cctatttcct atttagaaat tttattttat
    11941 aacttggaaa tctataccca tattatacgg cagaaaagaa aaattgcaaa cgcttttttc
    12001 attatttttg aattctttgc tcgatttcgc tttttgtttg cttgagggca ctgattattt
    12061 gttcttttgt ctcatctgtt aaacgatatt tcggcatact tacactaaat gcaccaatga
    12121 tttgttcgtt tttcacgatt gaggctccta tacaaaaaat atcacgttcg atttcttcat
    12181 cgtcaaatgc gatgtgggtt tcttttaccg ttttgatttc ctttttcaac cgtaatgcat
    12241 tggtgatcgt atattctgtg taagggatca acggatgctt ttcaagataa tcagtgtact
    12301 gctcttcact gaattctgct aatacggctt tgcccatcgc agaattgtat aatggtcggg
    12361 tgattccgat ttttgaagac atgcggatcg tttgatgttt tggatctagt ttgttcacgt
    12421 acaagatctc attgttgtcc aataccccta agtggatagt ctcatcgatc ttttgctgca
    12481 gctgttctaa aaacggtaaa gtcaactcta ccaaatcgat ttgttcaatg tttttattcg
    12541 cgtagcgaat aagctttgca cctaaacgat agtttttctc tgaattttta tttacgtagc
    12601 cgatcatcaa caaagtatct agtattttca agactgtaga agaggtcatt cccacaccct
    12661 ttgcgatcgt ctgcaaagag acatcttggt tttccgctaa atagtctagt attttagagg
    12721 ctttgatcaa tacagttcca taaggctttt tattttccat ataatattct cctatttctt
    12781 tataagaaaa attatagcgg aaataccatc aatttaaaag agatatccag atataaacta
    12841 ccatttctct ttacaggcaa tttaatgttg aagatccgct tcgtttttac atcaaacttt
    12901 gatataaagc aatgatttct tctactgtcg tttctcttgg atttcctggt gtgcatacat
    12961 ctcgcaaagc atcttctgca attgctggaa tatctgcttc ttttacacct aattcagaaa
    13021 tggtttctgg gattccaacc gctttgctca accgatcgat ctcaccacaa gccgcttctc
    13081 ttacatcttc taaagacatt tcagctgctc ctttaatccc tagaacaaga gcgatctcac
    13141 ggtacttttc tccagtatat tctttgttat atttcatgac agttggcaaa agtgctgcac
    13201 acgctacgcc atgaggaatg ttgtaccatg cacttaacgg gtgagccatc ccgtgtacca
    13261 gtcctaaacc gacatttgag aagcccattc ccgcaatata ctgtcctaat gccatttttt
    13321 ctcgcccatt ttgatcgcca tctacagaag cttccaaaga atggccgatg atttcgattg
    13381 cttttaaatg cagcatatca gtcatttccc aagcgccttt tgtgataaac ccttcaatag
    13441 catgcgtcat agcatccata cctgttgatg cagccaattt tttcggcatt cccatcatca
    13501 tatcgctgtc gatgaacgcg acgattggga tatcatgagg atcaacacaa acaaattttc
    13561 ggtgatttac ttcatctgtg atgacgtagt tgatcgttac ttctgctgct gttccagaag
    13621 tcgttggtat agctaatata ggtaaacatg gatttttcgt atcagcaaca ccttctaaac
    13681 taatgacgtc tgaaaactct ggattcgtca aaataatccc aatggctttt gccgtatcga
    13741 ttggcgaacc tccaccgatt gcgatcaaac agtctgcctc tgcctcttta acaaaagcca
    13801 ctccatcttt tacattttgg atcgttggat taggtacgat cccgtcgtat aaagcataag
    13861 cgattgctgc tttatctaga agttctgtta cttttgttgc tataccgtgc tcgatcaatc
    13921 ctttgtcagt aatgacagct gctttgccga atcctcgctg ctgaaattca gggacgatcg
    13981 actcaatcgc tccttttcca aaatacgatg tttcatttaa aatcattcga ttactcattt
    14041 tctattcctc ctaatttgta ttatttttca caataaaaac gaaagaaccc caagcaaccg
    14101 gttgcccagt tgcttgttgc tacagttcaa agactggcat caattcccaa ctgactggag
    14161 aggcatctgg atttgtctcc atgatatctt ccatgtactt ccaccatttt tgattgatag
    14221 atgtatttgc cattttttcc cataactttt catcttcgat cactaagtaa ccaaataaca
    14281 aacttgtact cggatcaaga tagatcgaat agtcgatgac cccgtgttca cgaagtgctg
    14341 tacgcatttc tggccaaagt tcatcatgtc tttttttgta ttcttcttgt ttttctgggt
    14401 aaactttcat gcaaaaagct tttttcacca tgtcagacac ctgctttcgt atgaacttcc
    14461 tccaaataac ctgcttttgg agtgacacca aacgcatctg ctaaatgcca caggttttcg
    14521 tccgtgattg tttgcaagat tggtccttgc gctttgacgt acgtatagac ttctgctgcc
    14581 ttttctgctg tctcgatcaa accaaagact tcatccatgt cttttcctga cccgtagatt
    14641 ccatgttgtg gccataaaac caatcgagtt tctttcattt tttcagctgt tgcttcccca
    14701 atctcatttg ttcccggaac catccacgga atgatggaaa ttccttctgg aaaaacaact
    14761 aagcattccg tacacatttg ccatagcgta cgactaaagg aacgttcgtc taattcatgc
    14821 gtaaaactca tcgctaatag atgactagca tggcaatgca taacgatccg attatctggg
    14881 tctacttcta aacgggcgat atgactcatg aaatgacttg gcaactcgct tgtaggaagg
    14941 cattcgtttt ccagccccca caaaagttcc agtgttttgc catcttcagc aactcgaacg
    15001 attccaagat tctcggcagg ttcgctcgct acatttttaa aatatcttcc tgaaccagtt
    15061 acaataaaat agcatcctgc taaacggctg gcatcaaaaa tcatcgagat gcggcggatc
    15121 actttttcag gatccaagaa cggtgtgatc tcttcttctt ttagtaaata agaaatattc
    15181 ccgccatttc tttcatccca tcccaagcga taaagattcg acgtcgtttc gatcatttct
    15241 ttcacataag gcgcttgtaa aatatctatt tttttcaata tagccacact cctattttct
    15301 taattccaac acgtcttttt gatattcctg gacttcttcc agccaatcca aaccaactgg
    15361 aacttgattt ttgtaacaga aataattcca tacatcggca tacgggaaat ctttcaattc
    15421 ttccgtataa gccattcttg aagtaaagtc aaaagccagc tccatttctt tcaatcgctc
    15481 aactggttcc agcatagctt tcagcagtgc tttttgtgta tttctcgtac cgatgaccca
    15541 agcagcgata cggttgatcg ttgcatcaaa gaaatccaga ccgatatttg ttttttccaa
    15601 caattcgtta cggactaatt ctttagcgat ttcctgcaat tcatcatcca tcaaaaccac
    15661 atggtctgaa tcccaacgca ctggacggga aacatgcaac atgattcctt tgctgaataa
    15721 agctaatgaa gataatttat tggaaatcac ttctgttgga tgaaaatgtc cagcatctag
    15781 acaaacaagt ttgtcacgag tcaacccgta acccatataa aattcatggg aaccgactgt
    15841 ataagcttca gcgcctaaac caaatagttt gctttctact gcatcttgta cataccgttc
    15901 atctaactct tctgtaaaaa tttcatcgag tgattccatt aaacgttccc gtggggtaaa
    15961 acgatcaatc ggattatctt tcatcccatc aggtacccaa aagttattga tacttgtttg
    16021 cccagtttct ttcccaaagt actccgcgat tttgcggctt cttttcccgt gttcgatcca
    16081 gtattcacga acttctttat ctggatgagc caatgtaaag ccgtctttca tcattggatg
    16141 cgaaaagaaa gtcggattga agtcaagacc gatttcttct ttttttgccc actctaccca
    16201 tttttcaaaa tgtttcggct caatttcatt tagatcgaca gattcttctg tatccaaata
    16261 gatagcatga aggttcaatt tgtgtttgcc cggtatcaac gaataagcct tctccagatc
    16321 ttgtctaagc tcttcaggtg ttcgtgcggc tcctggaaaa ttccctgtcg ccatgattcc
    16381 accggtcaat tcgccatccg gtgtcaagaa acctttgaca tcatctcctt gccagcaatg
    16441 catagaaatt ttgatgtcct gtatcttttc tagtacagca tctgtatcta caccgatagt
    16501 tgcatatttt tcttttgctt gttcgtaatt tctttcaatc gtgttcatac gattactcct
    16561 ttgattttat tcacgtacca ttccatcgtg ttttgcttat tttcatctgg gtaaaaagtt
    16621 tcacatggaa acgactgttt gattacattt cttgcttgtt ctatattcga aaactgacct
    16681 gtactgagca tttgcatggc aacattcccg attgctgtgg cttcactagg tcctgcttct
    16741 acaatgattc cggcataatc tgctgtcaat tgattcagta gacgattatt cgatccgccc
    16801 cctacgatgt gcagcttttc caatttcgtt ccagttaagt gttctacttt gatcagctct
    16861 tgtgcgtaac aaagagcaag attatcatac acacaacgag cgatagctcc cgatgagaca
    16921 gggactggct gctctgtttc aaaacagtat tgctgaatct cttctatcat atttggggga
    16981 ttcaaaaatc gagggtcatt gatttccatc acttgctgca gcggtttttc ttttgcagca
    17041 agctctgcca attccgcata actgtactcg tactcttgca ttcgggcaac ttcctggatc
    17101 aaccacatcc ccatgatgtt tttcaaaaaa cgcaccgtgt tatgggcacc ccattcattc
    17161 gtataatttt cagaaaaagc attgcgagaa acattggcga tctttgtttc aatccctaaa
    17221 agcgaccatg tcccactgct gatataaccc caacgttctc cttgacctgg cgttcctaat
    17281 acagctgacg ccgtatcatg acttgctact gtgataaagg tcacatctgg caaatcatat
    17341 tttgaaaatt gttctttttg cagatctcct agtatcgttc ccggctctgt caatggtgga
    17401 aataattcct tcgagacacc gattttttca agaagttcta tttcccattc ttttgtccct
    17461 gcattcagca gctgcatagt agatgcatta gttttctccg tcaccatgtt accagtaaag
    17521 acatacccta aataatcagg tatcagtaac aatttatgtg cttttgccaa attttctttt
    17581 tcttcagcaa acaattggaa caaggtattg aaaggctgga tctgaatccc tgtctgctca
    17641 tacaacttat ctagagaata gatttcagaa aactgttcta ctgctccatt ggtacgcttg
    17701 tcccgataag caatcggatc atttatccga tccccttgtt catcaatcag acagtaatcg
    17761 actgcccatg tatcgatccc cgcaaaaagg cgatcaatcc ctgtctgctt taatttttca
    17821 aatccaatca aaatctcttt gataagataa tcgatcttcc agcgatcgtg accgtctttg
    17881 ttccgaaagc cgtttttgaa ccggtgtact tcttccaaga tcaaagaacc ttttgcatcc
    17941 ctcgtacttt tcattagtcg tccgcttgat gcaccgatat cgatggcaac aaatgttgga
    18001 ttcatttctc cacctctttt actttattgt tgattagcac tttattaata aagcgttatc
    18061 atttattgac aatgctatca taatacagaa tcttatattt tgcttcacac tattttggta
    18121 tataattgca ttatcttgac aagttgggag tgtttacatg gattactggg aaaaagccaa
    18181 ccactcatgg tcggaagact caaaaagagt gatcttgacg cctacacaaa gaagcaggaa
    18241 tttatttttt tatatccaag aaatcggtta cttcaaagca ccaatccctt actatactga
    18301 acgagcacat ttaccttctt acttgataaa gtttactttg tctggcaaag gttcgcttac
    18361 atataatgat aatcagcaac agctaaaagc tggagactta ttttttattg actgccaaga
    18421 atatcagcgt tatcaaacga tcagcgaaga accatgggaa atggattgga tccatttgaa
    18481 tggtcagaac gtccaatcat tttatcagga gttcacaaaa gacggccata acaccttcca
    18541 ttcaacagga gatccatcag aaaacaagat ccaccatttg atcacagaat tgatccacat
    18601 gcaacaaggc acgaatgccc gtaccgattt ccagtcctct gtagctatcc acgagttgct
    18661 gaatgaactg attttgcaaa agtatcagct agatttcgaa gaagaggata ttcccgccta
    18721 cgtttacaaa ctgaagcaat acttagacga acatttcaaa gaaacgatta cacttgatca
    18781 attagaacaa caattcttat tgaataaata tcagctgagc aaagattttt ccaaatttat
    18841 cggttctcca ccaatcgatt atttgatcag caaaaaactt tcttacgcta aagaccttct
    18901 tcggtataca gaatttacgg ttcgaatgat ttccgaagaa atagggattg agaattttgc
    18961 ttactttagc cgcctgttca aagcaaaaac aggactatcg ccgagagact ttcgtaaatt
    19021 cagctgattc atataatatg tacgtcattt tttcaaaata ttttgaaata tatagataaa
    19081 aataaaataa ccgcagtttc tgcttctatg taaacaacag cagctactgc ggttattttt
    19141 tagtgatcat tctcattgtt catcttcaaa atgttcataa tgaaccaact gtttctgtgt
    19201 atcacgtttc aaaaattctt taggcgttat gccgaactct ctcttgaatg ctttcagaaa
    19261 atgcggataa gaagaaaaac cacttttact atatacagca ctagagctgc ggtattctcg
    19321 caacaagtgc ttggcataga tcagcttttt cttcattaca tactgatgaa acgtaaatcc
    19381 agtgtacttt ttaaattctc gtgtgatatg gtatttgctg acaaaaaact gcttttccat
    19441 ttcctctaaa gaaagcggat gagataaatt ttttgagata tattgtaaca tttgttccac
    19501 acgctcatta ggaactacag cattctgttc ttgcgcactc tcttcttttt gtacaacttg
    19561 attgagaaaa atcaagtatt ctaccagagc ttgttcatat tttatgtctt caccgtaaat
    19621 ttttttatca ggaggatggt cgataaatga taatttttgc ttcaatactt cctgttctat
    19681 ttgcaataca cgactcctct tctctcccat tggttgaaaa caagccgata gattagacca
    19741 tggcgtggaa cgactttcta gaaaagaagg ggtgacaaag atatagacac gctcgaatac
    19801 atctgaagat tggcggacga tacgatgcaa atcattggaa tggatgagca gcacacttcc
    19861 tgctttgatc gtaaattgtc gtccgttcaa ataaaaagtc gcttctccct ttaatgtagc
    19921 atggatctca tagaaatcat gatgatgata gatcgtttta ttttggctga cgacatcttt
    19981 gacatgatag atttcatact gatcagaaac catcgtattg acttgttcac cgctgatttt
    20041 cttttggagc atgtgccgca cctccttttg ttcacttatg cacaatttta ttataccacg
    20101 ttttttgtaa tcaaaaagag ctgcttttat atactcttat aaaaaagaag agacgtggca
    20161 tcttctagcc ccgccccctc cattctaaat attagttaac caacgtcttc tttacattat
    20221 tcaagaagac ttcttccata acgaatccca tcatcttttg tttcttcaag aaccacatac
    20281 aggtacggac gattcttcat gatgatttct ttgatttttg tgtactcgat cacaccgtct
    20341 cccaaattta ccggcacgat tttttcctgt tcgacacgaa aatctttcaa atgaaccgca
    20401 catatctttt caccaaaacg ttcaaaagct tcttctacca attggacttg ttcttgataa
    20461 tcactacttg tgatcaaatt ggtcggatct aaaatgattc ctagatagtc cgaatctact
    20521 gcttggatca gctgctctac ccttgctaaa gaatacaccg gatgattcaa acctggttct
    20581 atccccacca tcatttggta tttttctcca gcttttacca agcgacggat aacggacaca
    20641 gcttcagcaa atgcttcatc tgtgaaattt tcttccgtat attggatttc cggcaaaaca
    20701 catccagttt ccgaagcaac catcgacgca ccaaaggaag aagcatagcg aagatactgc
    20761 tcgaactttg tcagcaactc ttgtcgtatc gttaaatctg gatgaatcat attgatatag
    20821 caacttaaaa ttcctacggt cacttcttgc ttttctaaaa ctcgtttgac ataatttccc
    20881 atcccagaat tgatctcatt tgcgccagaa ggtatttcag gaaacgaaag acctaaggcg
    20941 agctgcagtg tatgaatccc ttgttctttt gttttttctg caagatcttc tatcgtttgg
    21001 acttcggaaa gatcatgtcc tctaattgta atgttcaaca taactattct cctaactcag
    21061 catattgggt agccacatga cgatttgcgg gaagtaagta atcaaaaaca agacgactaa
    21121 gataacccca taaaaaggaa gaagcggttt cagcattgcc tctgctttta cgcctcctac
    21181 acttgcaccg acatatagtc ctgtacctac tggcggcgtg atggagccta cacacagatt
    21241 catgattgaa aacaacccgt aatgtacagg atcaacccct acacttgtga cgattggtaa
    21301 aaatattggt gtaaaaatca agatagcagg cgccacatcc ataaacatac ccaccagtaa
    21361 taacacaaga ttgactaata acagaataat aaatttatta tccgttacgc ccataatcaa
    21421 cgaactaatt gcctgaggaa taccggtaaa cgccatggcg aaagacatca tggaagaagt
    21481 agccaataag aacatgatcg tccctgacat tgctactgat tgaaacaaca tattaggtat
    21541 atcttttagt tttaccgttt tatagtaaaa catagcaatc aacagggagt aaatgacagc
    21601 tatcgctgaa gcttctactg ctgtaaatat ccccgtcaag ataccaccga taatgatgac
    21661 aatcaataga aggctaggaa tcgcctcttt tattattttt ccagcatgag ccatctgtcc
    21721 tttttcgaca acagggtatt tattcttttt agcaacgatg aatgctacta gcatgattcc
    21781 tagcgcccac aaagcgccta cgagataacc tcccataaac aaagcagaga tcgaagcacc
    21841 accagcaaca agtgaataca tgataaaagc agtacttgga ggaatgacca tacctgtcgg
    21901 agctgacgcg atattgaccg ctgtcgcaaa cttccggtca tatccttctt ccacttgctg
    21961 agggatcaac actccgccaa tcgcggtaga agcggcaatg gccgagctag agatggaacc
    22021 aaataaagca ttccctaaca cattcgtatg agccaaagcc cccggcacgc gcccgacgaa
    22081 caacatcgca aaatccacta gtttcttagc tatccctcca ttattcatga taatgcccga
    22141 gagaacgaaa aacggaatag cgatcagcga aaaactattg atacttccca ccattttttg
    22201 agctgaacta aaagcagaaa catcaaacgg tataactaac aataaagtca tcatcgatga
    22261 gaatgcaata cttatagcaa tcggcatccc taatacaagc aacacaacaa atacaaaaaa
    22321 taacactaat ccggcttcta ttgccatttt attcctccat ttttatattt tttcttgaat
    22381 tgctggcttg ttgcttaatg tatcgattag attgatcagg ctatagataa tgatgagtac
    22441 ccccgataca ggtactgaca gataaacata tcccatagga atacttaaag aaggcgagat
    22501 ctgccccatt gtcaatgtga cagcattcca tccgccatac accatgatca tcgcagcaaa
    22561 taccagaaat aaactttgaa caacaacttc taaaatttta ctttttttgc cagttaattt
    22621 ttcacttaaa accgttacgg ctaaatgtgc tttttgtccc acaacatacg cggaagacaa
    22681 catggcaaac cagacaagac cgaatcgaac aagctcttcc gtgaccgtat ttggatttcc
    22741 taaaaccgtt ctggaaaata cttgccataa aacaacagcg accatcccaa caagcaaaac
    22801 agctcccagg atctctaata ccttgtttaa aatcattttc atcacagcta cgctccttgc
    22861 tcttggatct tttgataaag gtctttgtag gtaggatcgt ttttgaatat atcatgaagt
    22921 ggggcaactg cctctcgaaa cggtgtatta tctacatcat tgaattggac tccatgttct
    22981 tcttgtgcta aagctttttc ttcttcaatt gcttttgcaa aagcttcaat ttcaaaaacc
    23041 gttgattctt tcgctgcttc ttcaatggct ttttcctgct catcagttag ctgttctttg
    23101 atggcatcat tcatgatgat gatatctggc acacgtgtat gctcatcata ggaatagtat
    23161 ttagccacac caccatgtcc agctgtatac aagacaaatt cattgttttc tgatccgttg
    23221 atcaaattgg attgaagaga cgtgtacact tcatcacttc ccataggtac gggcgaaccg
    23281 cctaaaagct ctaccatttt gatagcggtt tcgctttgca tcacacggat cttctttccc
    23341 ttcaaatctt ccggcgtatg gatcggaccg tctaccatat aaaagctgcg ctgaccagaa
    23401 tcataatagg tcaatccggt aaatcctaaa tttttggtcg attggtagat tggctccatt
    23461 atttcttcat tatccatcac cttaaagaaa tgttcttcat cattgaatag ataaggaact
    23521 gaaaaaatcg aataatcttt agagaaactt tccaacgctg aggcacttac ttttgtgaaa
    23581 tctactgctc ctgactgtgt caattcgatc agttcacgtt ctccacccag ttgtccatct
    23641 gggaaatact ggatctgtac ggaaccatct gttttttctt ttactaaacg cccaaattca
    23701 ttcattgcat caattactgg ctggctgttg cttgcataag caaagcgcaa ggtaactatc
    23761 tcatttacat ctgcatctgc gacagttgcc acactagaat tatttccgca gccgcttaga
    23821 cctagcagac ctaggctaag cgaacttgct gcaaacagca gtgatgtttt tttcatatgg
    23881 gtttcctttc ttttaacaat aagagacgag caattctgtc aaacaaagca ccgttaacga
    23941 ctgtccataa ggcattgctg tgattccgat attccgataa aaatctaaac tgtcccccat
    24001 tcctgtcccg attgaaacgt tttgaacttc tcctttttca tcgatttgtt caagcaatcc
    24061 ttggatagca cgataagcga cttctttata ttcttgtggt aaataacgtt tatggaccgc
    24121 tttcaaaata ccgtaagcaa atcctgcagt tgcagaagat tctacgtaag aagaaggatc
    24181 atccaacaac gtatgccata aaccgctttc agactgataa gtttttaatg cacgcacttg
    24241 tgcttcaagt gtttcgatca ataatttgcg caatgcatct tctttcgata attctaagat
    24301 ttcaatgatc tctggaatag caatcgtcaa ccagcagttt ccacgagccc aaaatgcttc
    24361 ggcatagtta tgatttcctt caaacgtcca gccgtgatac cacaatcctg ttcgtttatc
    24421 catcaagtat ttgatatgga tcataaactg gtaacgagct tcttctaaat attctgggcg
    24481 attcaacagt ttcccgattt ttgccaaagg aagcactgtc atcatcaatg tatcgtccca
    24541 caactgattc ttattttccg gtccatatgt ggcatgctgt aacccgtctt catttgtccg
    24601 aggcatgtca ttcatgaccc attctgccca ttgttctaaa taaggcaaat agcgtgaatc
    24661 tttcgtgtct tcatacaagt aagccatagt caatagcggt gccattgtat tgacgttttt
    24721 gggcggagcg ccttcttcca tacgatcctc aaaccattca ttgaccactt gataagcgcg
    24781 aggatttttt gtcatctggt aatttttata aataccatat agaccgacac cttgtggcca
    24841 attccacact gtccagcttt tgtcatctac tttcagtcca tcaaagtcca acaaaaattc
    24901 accattatcg tctttgatcg ttgtcaggtt ttcaacgagc aggtcaattt tttctttcac
    24961 taattcttta ggtacaaaaa tctcttcttt aataatcatc tttacgccac ctttttaaac
    25021 gctttcattt tatatcctta atatagcagt aataccgtcc actataaata tcgctttttg
    25081 aacaaaaact gtctaaaatt gacctaaatg ttaaataaca aaagtaactt tcagttactt
    25141 ggaaattaaa aaaagaactt ggagaaacaa cgttttccaa gttctttttc atctattttc
    25201 ttatcttttt tctacgcttt cttttgctta tccgttgtaa gcatcaatga tgatctgttt
    25261 caattcagag atcaatggtt ctttcggatt agctgtcgta cattggtctt cgtaagctaa
    25321 ttcagccata cggtctacgg ttgtatctag tgtttcttgt gtgactcctt gagcttttag
    25381 actcatgttg atgccgacag attttcctaa ttcataaaca gccgttgcta aagcttcgac
    25441 taattcctct gttgtttcgc ctttcagacc taagaatttt gcgatatctg cgtaatctgt
    25501 atctgcacgg aaatagtcat atttagggaa catcgcatgt ttttgtggat cttttgcatt
    25561 atagcggata atatgcggta aaagaatcgc atttgtccgt ccatgaggga tcccgtattc
    25621 cccgccgatt ttatgtgcga ctgagtgaca aatacctagg aatgcgttag caaacgccat
    25681 accagccatt gtagaagcgt tgtgcatttt ttcacgtgat tccatatcag gtgtttttac
    25741 tgatttttct aaatagtcaa agactaactt gattgcttgt aggctcaatc cacgtgtata
    25801 atctgaagcc atgactgaaa cataggattc gattgcatgt gttaacacat ccatcccggt
    25861 atctgctgtg acagatgctg gaactgacat gacgaattga ggatcgacga ttgcgacatc
    25921 tggcgtcaat gcataatctg ctaacggata tttcacatgt gtttcactgt cagtgatgac
    25981 cgcaaatggc gttacttcag aaccagtccc tgatgttgtc gggatacata cgaattgtgt
    26041 tttttctggt ttgtcgatct tatatgtccg tttacggata tccaagaatt tttgttttgc
    26101 accaaagaaa gatgtgtctg ggtgttcata gaacatccac attcccttag ctgcgtccat
    26161 tgcagaaccg cctccaagag cgattactgt atctggcttg aagtctgcca tcagtttcgt
    26221 tcctgcgtat acggtatttg tcgatgggtt cggttcaaca tctgagaaga cttcgatctt
    26281 cacatcattt ttacgtcgtg ccaatacttc acgaactgta tcgcagtaac cgaattggac
    26341 cataccaggg tcacaaacga tcatgacacg ttcaacgttt tccattttct ctaagtacaa
    26401 tagagagttc ttttcaaaga agatttttgg cggtaatttg aaccattgca tgttatttct
    26461 ccgtttcgct acagttttga cattgattaa gtttacagca gagacgttac gagaaacaga
    26521 gttcttacca taagaaccgc atcccaaagt caatgacgga atcatttcgt tatagatatc
    26581 accgatgccg ccttctgcag atggtgtatt cactaaaata cgacaagctt tcattcgaag
    26641 accgaatttg acatgcaact cttcgttttc tgaatggata actgctgtat gtcctaatcc
    26701 tccaagattc aacatacctt cacacaaatc aaacgcatgg tccgtgttgt tagctttgat
    26761 catggcaaga actggagaaa gtttttctct tgatagcgga taatctgggc ctacgccttc
    26821 caattcagca atcagcattt tcgttccttt tggtacactg attcctgcca attttgcgat
    26881 ttccatcgca gaatgtccaa cgattgatgg attgaccgca tattttcctt cgttcatcac
    26941 tgcatcttct aatttttgca attcatctgg ttttacgata taaacttggt gtgcttggaa
    27001 ttctgctttt actgctgcat agatttcttt atccacgatc acagcttgtt cagaagcaca
    27061 aatcattcca ttatcaaatg ttttcgaaac gatcaaatcg ttcactgcac gtttaatttt
    27121 tgctgttttt tcaatataag ctggtacgtt acctggacct acacctaaag ctggttttcc
    27181 tgtagagtaa gcagacttga ccattccagc tccacctgtc gctaagacga tcgcgatacc
    27241 tggatggttc attagcatag aagtcgcttc gatcgaagga tgttcgatcc attggataca
    27301 attttcagga gctccggcag cgatagcagc atcacgtacc actcgtgctg cttctgcaga
    27361 agatttttgg gcacttggat ggaaagcaaa aacgattgga tttcttgttt tcaacgcgat
    27421 cattgatttg aaaatcgttg tggaagtcgg attcgttgtt ggagttactc cgcaaaccac
    27481 accgactggt tcagcaattt caatcaaccc tttttggaca tcttcattga tcacaccaac
    27541 tgttttatca tgtttgatat tatgccaaat atattcggac gcatacatat ttttgattgc
    27601 tttatcttcg taaacaccgc ggcctgtttc ctctacagcc atcttcgcta atggcatatg
    27661 ttgatccaaa gctgccattg ccatttcatg cacgatatgg tctacttttt cttgatcaaa
    27721 gctttccatt tctttcaatg ctgcatttgc tttttctgcc aaatcatcaa tcattgcttg
    27781 tacgtcaatt gtcttcgttt tttctttttc tattacttta gccatcatta tcctcctaat
    27841 gggtcatggt tatttgttaa ataattcaca tgtatatcat acaatattta tttttctatg
    27901 taaacacttt tttgtgattt ttttcacagt tatttttaaa tataaaattg ctttatttac
    27961 aggtgtttgt atagaaaaat atataaagtt atttattatt atttatatga cacacccgat
    28021 tattcaatat tgagcaatat atcccatgct cttatctctt tcttttttga ataaaaaatg
    28081 aggtaggaca tctttctgcc ctacctcgtc tttaaataag ctttgatact tatttttctt
    28141 ccttctcttc tgtctcttcc agcgtttctg ttgtttcagt ttttacttca actgtctcag
    28201 ctggttcttc tgttgtttca acagtagtcg ttgcatcatt tgttacgaca gtccctggtt
    28261 tgaccgtttt gattgctgca cgatcgtact caaggaagat cccttcgcag tctaaaacaa
    28321 ctgtccgttt gtcattgtcg atttctgaaa caactccatg taatccgccg atcgttacga
    28381 ctgaatcacc tggtttcatg ctatctaaca agttttgacg ttcttgctgt tgtttctttt
    28441 gcgaacgtga cataaaaaac cacattgcta atagcgcgat aaacatcaca agcattggta
    28501 atgttcccat ttttttcacc tcactaattc tgtatctaca aaaaacactt tagcagaaaa
    28561 atgctgataa cactagtttt ttacttaatt tttaaaagat tttcctttag aagctttttg
    28621 cgttttcttt attgaacccg tattcttcaa aaaaggcttc tctaaattca agcaaattat
    28681 catccatgat tgcttgacgt acttgtttca ttagattcaa caagaagtat aaattgtggt
    28741 aagaagtcaa acgaatccca aatgtttcat cacatttgat caaatggcgg atataggcac
    28801 gtgtatagtt tttacatgta taacaatcac atttctcatc aattggacgg aaatcatggg
    28861 catatttcgc atttttcact actagacggc cttgagaagt catacatgtg ccatttcgag
    28921 cgattcgagt aggcaagaca cagtcaaaca tgtctactcc acggataaca ccatcgatca
    28981 atgaatcagc agcaccgaca cccatcaagt aacgtggttt gttttcgggg atcaacggtg
    29041 tagtgaattc taatacacga ttcatttctg atttaggttc cccgacagat aacccgccga
    29101 ttgagtaacc agggaaatcc atcccgacta gatctttcgc gctttgacga cgaagatctt
    29161 caaaccctgc tccttggatg attccaaata atccttgacg gtctggatta gcatgagctt
    29221 tcaatcctcg ttctgcccat cgagacgtac gttctactga ttttttcacg tagtcgtaac
    29281 tttcatcgaa tggtggacat tcgtcaaaac tcatcatgat atcagagccc aatttatttt
    29341 ggatgttgat tgctttttct ggtgataaaa acatacgcga accgttcaag tgatttttga
    29401 aatgcacgcc ttcttcttcg atattccgca tatcacttaa tgaaaaaact tgaaagccgc
    29461 ctgaatctgt taaaatcggt tgatcccagt tcataaattt atgcagtccg ccagcttctt
    29521 ctactaagtc ttcaccaggg cgtagccata aatgatatgt attacttaaa ataacgccag
    29581 cacccatttc tttcaactct tctggggaca ttgtttttac tgtagctaat gttcctactg
    29641 gcatgaacat tggtgtgggg aaagttccgt gtggcgtgat caattcccct aaacgcgcgc
    29701 cagtatgttt ttctttcttg attaaacgat agcgaatggc aggttgcgtc attcatttcc
    29761 ctactttcta tataaaatat cttgacaact cccaccatca taatcgtttc aagtgctttt
    29821 tgcaagttcc ctcggcttcc ttctacttta tttaatattc ttttttttat ccttttttta
    29881 tgaataacaa aaaaaagaaa aacattatga cagaaatatt ttttgataca tggtatattg
    29941 gtgttattaa agaaaaggag ggaaaaatca tgcaatcttc tgcagaatta aaacgccaag
    30001 caaaagagag tctaaaagga cgttggggac aagctgtttt actaaatcta atccctacat
    30061 tgattggtat tgctactatt cttcttatta tgattcctat tatcttgttt gccaccacca
    30121 tatatgatgg atcagcgagc gtctcagaag cgacaacttc aggaggcggt agttcaggag
    30181 gcggtggcgg aattatttcc agcatcatta gcgctttgtt catgagcggt atttcttgga
    30241 cttatcttga tattttacgt ggacaaaaag atcagatcga accattcaaa gatgcatttc
    30301 gtggattcaa aggtttgttt atcggcggag tgatcagctt agctgtgctg atctcgatct
    30361 ttacaacatt atggggtttg ctattagtta ttcctggaat cattaaaagc tattcttatt
    30421 ctcaaagcta ttttatttac tacgatatcg tccaagaaac aggacaaaca cctaaagtac
    30481 tggatacgat cactgcaagt cgaaaattga tggatggtta taaaggacaa cttttctggt
    30541 tagatctttc atttatcggc tggcatatct tagcattagc aactgctggt attggttatc
    30601 tatggctcag cccatacatt tctgcaacaa aagcagcatt ttatgaacaa ttgccaaaag
    30661 atatttaaaa gagagtgtga cttgtgaccg agggggtggc aggagaacac cgtttatcga
    30721 gaggcagtaa aagaaacatt cagcccatta gcaaaaagga ggtgtgacaa cattcatgtc
    30781 acacctcctt tttgtatagt tccagtaaaa agaaaatcaa cttagaaaca gttaatcatt
    30841 cctcttcttc tgcttccggt aatcgatgga acttgatcag ttcattcacc gcgattggta
    30901 cgatagataa aatccctacc aagaaccagt gattcaaact gatgtgtact aggccgaata
    30961 gttcttgcgt aaatggcagc aaagcaacag ccgttgtaat agctaatgcc agtaacgcca
    31021 tttctaccaa agctttattg gtcgctaaat tgactttgaa gatcgattgg ctgctgcgta
    31081 cattgaacac atgcagaatc gatgaataag ctaagatcaa gaatgcaaca gtttgaccga
    31141 catgctgact tgcgtctacc catgggctga tagtatcgac ataactacct aaataataac
    31201 caaacaaggt tacgatggtg aagacccctg catttatggc aattttttgc aataaacctc
    31261 gagcaaagat cccttcattt ttcggaatgg gtggctgatc catgatgccg tgttccgctg
    31321 gctctcttcc taaagcaaat cctggaagtc catcagcaac tacattgaca aacagtagtt
    31381 gcacagctgt aaatggtgct ccccatccta acagcatcgc aatcaaaaca ataaagatct
    31441 ccgaaatatt acagcttagc aaaaagttga tagctttacg aatattctga taaaccgaac
    31501 gtccttgact gacagcgtct acgatcgtcg caaagttgtc atccgtcaaa atcatatctg
    31561 cggcactttg cgccacatct gttcctgtga tccccatggc acacccaacg ttacttgctt
    31621 tcaacgctgg tgcatcattc acaccatccc cagtcatcgc tacgactgca cccgtacgtt
    31681 gccaagattt gacgatccgg atcttgtctt ctggcgttac ccgagcatag acagagagag
    31741 ctttgacacg cgcatctaat tcttcgtctg ataattgatg cagttctgat cctgaaagtg
    31801 cttctgacgg atcattcaag atacctaatt ctttcgctat ggcactggca gtcaccacat
    31861 gatctccagt gatcatgact gttttgatgc cagccttttt cgctcgcttg attgctcctt
    31921 tactttctgg acgtggtgga tcgatcattc cgatcagacc aatcaaacgt aagtcttttt
    31981 ccagtgcttc agaagaaata tcttgcggtt cttcgtcata agtagcataa ccaacagcaa
    32041 tcactctcag tgcccgttta ccaaagcgat cattaacgat cgccgcttgt tctacatcgc
    32101 ctgaactaaa gcgcggagca agaacatcga atgctccttt ggtgattgag atgtattttt
    32161 cgcccatttg gtgaactgtt gtcatcattt ttcgttcaga atcaaaagga agctcgttta
    32221 ctcgtggata tttttcttct aattctgcct ttgtatgata attctcttca acagccctta
    32281 cgatggctac ttctgttgga ttacctttga taacaaggtc gtcatcttct tgctcgaccg
    32341 tcacatctgt acatagtgca gccattttca atatttccat cgcgctgttc gtcattgaat
    32401 cttcgatatt cgttacttca tcttctttgc tccatacccg gcggacgcgc atttcatttt
    32461 gtgtcagcgt tcctgttttg tctgagcaga taacattagc ggttcctagc gtttcaactg
    32521 ctggcaactg tcggatgatc gcatgtttcc tcgccatctt ctgcacaccg tatgcaagtg
    32581 tcaacgtaac aataacagtc aaggtttccg gtacagctgc aacagccaag gaaatcgaag
    32641 tcatgaacat ctcaaggagt ggttctcctt gcagttcacc aatcacaaat actaaagccg
    32701 cagccgctaa agcgatcata ctgattcgtt tgcctagctg attcaatcgg acctgtaaag
    32761 gtgttttttg catagtattt tcgttcaaaa gatccgcgat cttacccatt tctgtagcca
    32821 tgccaatggc agtgacgatc gctttcccac gacctgcagc aaccgtacat cctttaaata
    32881 ccatattaag ttggtcacct aatgactcat cttcttcagc aataaatgct gcatcttttt
    32941 caactgcttc actttctcca gtcaacgccg attcttctac acgtaattgt gatgcttcga
    33001 tcaaacgggc atcggcagaa atagcatcac cggactctaa aacaagaata tcacccacta
    33061 ccagttgctc tgactcaaca gtggtcactt tgccgtctcg tatgacagtt gccgtttgtt
    33121 tattcatatc ttgcaaggat tccagtgctt tttctgcgtt tccttcttgg acaatcgcca
    33181 ataccgagtt gatcacaacg atagcgatga tcagcagtcc ttcaaataaa tccccatgtt
    33241 ctgtagcaaa tgcggtataa aaggaaatca ccgctgctac aagaaggatg atcgttgtaa
    33301 aatcggacaa gcttcgaaag aattttttga tcatcgattc tttcggcgcc tcatcgaatt
    33361 tattcaatcc ctgctttttt cgccgttcat ccacttcttg tgaagacaac ccagtatctg
    33421 gatctgcttc tgttaccttc tttacttctt ctattgtctg cttgtattct tccatcctat
    33481 tcctccttta aaatcttcgt acaaaaaaag gtacttattt tactataaca tccttttttt
    33541 atttacttca aataaagggc ttatcgatta tttgttaaga aagcgtaaaa tttaaagtgc
    33601 ttatatttgc aaacgctacc aaaggatgct attatataga tgtaaggaag gaggatacca
    33661 tcaaatagac agaagaattt ctcgatagaa cagtgtaagc aaacaaagtt gttagaagta
    33721 caggtttcac ccagacatgc gtgtagagta caaacctata aatagtgaat gcgtatggat
    33781 gtttggatca cgaagtatcc gaaacaagaa gtaatgcgat acgtaacaat tgaataacgt
    33841 aaacgtctga aaattgaata ggaacaagag atgtttcaaa tgttgaagat gtaacgaaaa
    33901 caaaattttt gtttaacatg aagaaattca acaccgtgct ttgcatgtat gatggaaagt
    33961 tacaatcatt tctgtttatt tgaatccttc cgaggtacaa gaaagccgag cttatcgaga
    34021 gctcggcttt ttctttgtca taaaatcttt gtaaagcttt tttgttattc aaaactagcg
    34081 gcttttcata accttatttc acgaacatcg catctccaaa actaaagaaa cgatagcgct
    34141 cttcaatcgc atgctggtaa gcagataaag tcaattctct tcctgcaaag gcactaacta
    34201 acatgactaa agtcgatttt ggcaaatgga aattagttga gaaagcttct accactttga
    34261 attcatagcc aggcgtaatg aaaatatcag tccatccgct gtctgctttg atttctccat
    34321 taaatttcgt tccaatcgtt tctaatgttc gaattgaagt tgttccgaca gccacgattt
    34381 tcccgccatt tttacgtact tggttcaatc gctcagccgc ttcttccgtc aaacggtaga
    34441 attcactatg catatggtgt tcttcaattt tatctacgct gacaggtcgg aatgtaccta
    34501 accctacatg tagtgtcaga tagactaatt caacaccctt tgcttcgatc tcatcaagta
    34561 attctttcgt aaaatgcaat ccagctgttg gcgctgcagc agaaccattt tctttcgcat
    34621 agacagtttg atagcgatct gggtcttcca gacgttcttt gatatatgga ggcaatggca
    34681 tttctcccaa tgattcaaga atctctagaa aaattccttc atactcaaag tcaatgatcc
    34741 gtccgccgtg ttccaattct tctttcacta ctgcttgcaa tctgccatca ccaaaagaga
    34801 ttcttgtccc aactttggca cgtttcgcag gtttgatcaa tgtctcccac gtatctcctt
    34861 gtgtattcgt tagaagaagg acttctaaat gcgcaccagt atctggtttt tctccatata
    34921 aacgagcagg cagaactcgt gtattattca taactagcgc atcaccaggc tgtaactctt
    34981 caatgatttc atggaaatgt ttatcttcca tctctccagt tttccgatcc aaaacaagta
    35041 aacgagaatg atcgcggtct tttaatggtg tttgtgcgat taattcttcc ggcaagtcaa
    35101 aatcaaaatc ttctgttgtt aacatatcgt cactcacttt ctatcatgtg cttcatcatt
    35161 ctatcatgtt tttcgttctg ttggcaaaat cagcactttt tcacttctat tttttgtttt
    35221 cctaaataaa aaaactaccc tatttgtatt ttttcattgt tttaggcata ctagtaatag
    35281 atagttcttc attgaaaata gaaaaagagg tggcaccatg attgaatttc aaaatgtgtc
    35341 aaaaatttat aaaggtggaa aagtcgctgt agaggacgtc aatctttcat ttgaaaaggg
    35401 tgagttcatt tgttttatcg gtacgagcgg aagcggaaaa acgacgacga tgcggatgat
    35461 caaccgaatg actgatccaa cacaagggaa aattttgatt gatggaaaat ccatccaaga
    35521 aattgatcca gtggaattgc gacgtcagat cggctatgtt atccaaagta ttggattgat
    35581 gcctcacatg acgatccgtg aaaatatcac tcttgtccaa aaattgttga aagtcagtca
    35641 agaagaacgg aataaaacag ctgaaaaaat gattgatctt gtggaattgc cccgtgagat
    35701 gttagatcgc tacccacatg aattatctgg tggtcagcag cagcgtattg gagtcgttcg
    35761 tgcccttgca gcgaatcaag atatcatctt gatggacgag ccattcggtg cgcttgatcc
    35821 aatcactcgt gattccttgc aagaccttgt caaagattta caagagcgtt tgggaaaaac
    35881 gatcgtattt gtaacccatg acatggatga agcgattaaa ctagcaagcc gtatcgccat
    35941 catgagtgaa ggccgtgtga tccaatttga tacaccagaa aatattttac gtcatcctgc
    36001 aaacgatttc gtagaagagt tgattggaga agatcgccta ctacaagcca agccaaatgc
    36061 gacaagagtc ggcgaagtta tgctaaatac agcaatcacg attacaccgg aaaaatcatt
    36121 acaagaagca atcaaactga tgagggaaaa acgtgtagat acgttgttag ttaccgataa
    36181 ttcgaatgta ttaaaaggat ttatcgatgt agaaacgttg aataaaaaac gaggaaaagt
    36241 atccagtgtc ggcgacattt tgaataaaca tgttttttac gtaaaagaat ccgcctatct
    36301 tcgtgatacc ttgcaacgca ttttaaaacg cgggctgaaa tacgtgccgg tcgttgatga
    36361 acagaacaaa gtcgtaggta tcctgactcg tgcatcatta gtggatatcg tctacgatgt
    36421 gatttgggga gaagatcccg caactactga tgttgcagag tcagctactc ctgaagaatc
    36481 taaggaggtc tgacctatgc agcagttttt tcatcagcac ggttcagaaa ttctctcaaa
    36541 aggttttgaa catctttata tttcaggact tgctttgatc attggtatca tcattgctgt
    36601 accgcttggt gtcttactga cacgttttcc aaaagctgcc acgattgtga ttggcttaac
    36661 cagtgccttg cagacaatcc cttctcttgc tttattggca ttgatgatcc cgctatttgg
    36721 cgtaggtaaa ttacctgcga ttatcgcact ctttatttat tcattattgc cgattttacg
    36781 taatacgtac attgggatga aaaatgtcga ttggaattat cgggatgtgg caaaaggcat
    36841 ggggatgact gagttccaat ccattttttc cgttgaattg cccattgcta tgccaacgat
    36901 catggctggt attcgtttag ctgccgttta cgtgattgca tgggctacac ttgcctctta
    36961 tatcggcgct ggtggtttag gcgatttgat ttttagtgga ctgaataatt accaaccaga
    37021 tctcattttg gcaggtacga tccctgtaac aattttagca ctattggctg attggcttct
    37081 aggtctcttg gaacaccggc tgacaccgat cgcacttaga gaggagaatg aagaatgaaa
    37141 cgtatactaa aaatgaccat ggttcttttc tctcttctta tacttgcgag ttgttccttc
    37201 cctggtcttg ccagcaatac agatgaagac accatttcga tcacaggtgg aatcacttca
    37261 gaagcacaga tcttagcaag tctagttgcc ggaatggttg aacattatac agataaaaat
    37321 accgcaatca tcaacaatct ggctactaca actatcaacc atcaagcgat gatgaacgga
    37381 gatgcttcaa tttcagcggc tcgctacaca ggaacagatt taacgactac attgaacctt
    37441 cctcctgaaa aagatccgaa aaaagcgttt gctaccgtga aagatgaatt tgagaaaaga
    37501 tacggtcaaa cgtggttccc gtcctatagt tttgaaaata cgtatgtctt tcttgttcga
    37561 aaagacaccg cacaaaaata ccatctatct aaagtcagcg atctgaaaaa tgtagcggat
    37621 gaattagtag ctggtgtaga tacttcttgg atcaatcgta aaggagacgg ttatgacggc
    37681 ttccaagaaa cttatggctt ctccttcaat tcgatattac ctatgcagat tggacttgtt
    37741 tatgacgcag tcgaagcagg aaaaatggat attgttcttg gctactctac ggatggacgg
    37801 atcgccagtt atgatctagt catgttggaa gatgatcgtc aattcttccc accatatgat
    37861 gctgctccag ttgtagacaa caagatatta gctgatacgc ctcatttaaa agaagcctta
    37921 aataagttag gtcataccat cagtacggaa aaaatgcagg aattgaacta cgaagcagat
    37981 aataatctag tggaaccatc agtagtagca caacgtttct tacaagaaaa ccactatttt
    38041 gaagggaagt gaagcaattg gataaaatga atgtctttca acaatttttc tattatttcc
    38101 aggaaaatgg ctcctatatc tttgcacaat ttatccgtca cttcctgatt tctatctatg
    38161 gtgtcctctt tgcagcagtt gtcgggattc cggttgggat catgatttct aggagacgta
    38221 aattagcaaa ctgggtgatt cgtttggcga atatcatcca gacgatccct tctctagcca
    38281 tgatctccat tttaatcatt ggattaggcc tgggagtcaa tgtcgtgatt gttactgtat
    38341 ttttgtattc cctcttaccg atcatcaaga atacgtatac cgggatgatc caagtagaca
    38401 aaaacatcct tgatgtcggc aaaggcatgg ggatgactgc tagacaacga ctattcatgg
    38461 tagagttacc actctctgtt tctgttatca tggcaggcat aagaaacgcg ttagttgttg
    38521 ccatcgggat aaccgcgatc ggtgcattcg tcggtgctgg tggtcttgga gatatcatta
    38581 tccgaggaac caatgcaaca gagggcacat cgattatttt agcaggtgca ttgccaactg
    38641 cattaatggc gatcatcacg gactggcttt taggtatttt ggaaagacgg ctagatccag
    38701 ccagccgtac atcacgctga tgttattgta aaaaacaaaa gcttctgcga ccatttgttg
    38761 gtcgcaaagg cttttttagt tctgtcattt ttaaggcaat aatccactct gattcttccc
    38821 tccttatggg taaagagttg cttttcttag ctatttatcc attccgtata ctccaacttc
    38881 atactgccaa cgggttcata tctgcatcca ccgactaagt ttttttctta tccgctaagg
    38941 cattttgcca atccattttc ccatcactcg tcatggaatt cataccacat gatctctaaa
    39001 tactcgtctg cttgttcttt gaattgggca tcaattctta catcctttgg cgtggctttc
    39061 atttaagcct tttccatcag tcctcgattg ttcgatgcct tcagctgcat catcaaaact
    39121 ggtgactgcc gtatcggtaa cttcgatcat cttttataca atcgggaact tgccatcctc
    39181 atccttggct aaaaacatat ctagccattc cagcttctcg ccatgggagg tttgcggttc
    39241 ctaaaaccgg tacacgattc ttatggactg catccgtcgc aactagactt ggcgatacga
    39301 tgatccttca ccagaagagc acccccgtaa accaattgat cgatatattg ccaataagaa
    39361 aagacattgg cgtaaaagat atcgatccct cgtgatgcat tgccgttgat actgctattc
    39421 atggccgaaa gcgccacgac tttaacttct tccgtttgag tgacattcat gttcggcaat
    39481 gtcgctttgg atacccgctc tgctaaagga acgacactgg tattgtattt cgtatctgta
    39541 tcttttccaa aagaccatgc caacgaatcc tctgggaacc aataagacga ctccggttga
    39601 ttgtccattg cttttttcaa catacttttg gctgtttctc gataaacgac atttccaatt
    39661 gtcttcgcct cactttggcg ataatcgatg gtcgttgcca ctgccaacac taacaacgat
    39721 gctccaattt tactgatttt ccttgtcaca cttcttcctc cttgttttac ataaacccgt
    39781 caacattcca ttcctaaact caaacaatga cccttcaaag gtggagatat aggaacaaat
    39841 taaagaaatc atgcaataaa tccaagaagg ttggttttag aacaaaaaaa gagtctcctt
    39901 tttggggaga ctctttgtta actatctaaa aaggttggct cttctcactt cactcataat
    39961 gtgtccaggc agaataaatt tctacaactt tattttcctc atcaactttg taaaccactc
    40021 gatgctgtct attcagcctg cgtgagtatc tgccttcatg tgtaggtctc agcttttcaa
    40081 aagactgcgt gggcatatat ggatcttcct tcaatgtctg aatcacttct tcaaattgtt
    40141 tctttaaatt cgtttgcttg atttttctta aatccacttt cgcagagttt ttgattgcga
    40201 ctgtatagtt actcatcaaa gagcttccca atcaatatca tcgatattcg taaagctact
    40261 gtcgtctttt tcgcggtctc gtactttatc taaagtacct gtttgctcaa gaaaaagggt
    40321 ttcttgaatc gctctccagt cttccagccc aatcaataca gcattgtttc cactacgatc
    40381 actaataatc tcgatttcag tatgattttc atttacctct ttcaataact gataaaaatt
    40441 tttacgagca gtgctaggat tcatcgttgt attagccatt gtctctcctc ctttttctga
    40501 tataattata gcgtacttga ttaagtacgt caaaaaaata caataaagca cttaaattca
    40561 gcaaactttt cctctttttt acatagagaa aaagacccct ttttagggag gatctttgtc
    40621 cacgctcact tcttactcct ttacgactag cggtagccaa acgtaaagta attggtaaca
    40681 acgtatcatc tttagcaaag gtcgaatcca aagcctgtga gtctatgcaa caacaccaat
    40741 acacgaaggt tcctcgttgc agtgtgactt gattgatatc ttgaataacc tgaggatacg
    40801 cacgaaagat ttgtaacggt tattcaaaac tgattttggc cgtcaagtct tctactgcct
    40861 caaaaatgat tgcctctttc gtctcctgtc attcatgttc actatcgatt tgatacttgc
    40921 ttagaccgtt gggtttgtga atcaccaatt aaaaatggca ctgagttatg gtaacaaaaa
    40981 atggtcactt gacgaggctg agattcctac ttatgaagac acatggcact taagagtcga
    41041 ggtaaaggaa gaagctgaaa atataggagg ctgtacatta tttggcaaag gattcggaaa
    41101 ttatgatcaa gacatcaaaa tcaaaatgtt ttacagctaa ataggaagaa taataacatt
    41161 tcatacgctg ttcccatctt taatggctta tagagggcac ctttagctga gaataagaca
    41221 aaacagaccc tctagcttca attaagagct agagggtctg tttttgattt taaaacttgg
    41281 tttatttctt caaatggtag ctataggtgc tgacaacgat attttcacgc caagacaatc
    41341 gcaatcttaa ccgcaaactt gtttggttat ggagaatatt ctgccagcgt ctgtttggta
    41401 caaattgagg aatcaacaca gtagttgtat agttttgttt cgctgcattt ttacttacga
    41461 tatctacata acggataatt ggattttcaa tcgagcgata agaagagtgg acgattgaaa
    41521 aacggacatc tgggaaatgc tttttaaatt ctgcttcgat ttctttttct ttttcgacat
    41581 cttcgtctag cgaaacatgc atcgcaacga cataatcacc gatcgaacgt gcgtaattca
    41641 acgcaccgat atttacccgt gtcacattcc cgactaatac aagaacggta ttcccgtcat
    41701 aatcatgtag ttttgcatca ttttctaaac gaagctgttc tgctactttt ttataatgat
    41761 catggatctt ataaaaaatg aacataacga ttggcataat gatgaagaaa ggccaaatgt
    41821 cccctaaccg atagacaaac aaaatcgcaa tgattgcata tgagatgaac gcacccgtaa
    41881 tattggcaat cgatttgctt aaccatcttt ttccttcttt tttccatttg acgaccatcc
    41941 ctgtttgaga taaagcaaat ggaatgaaca cgccgataga gtataacgga atcaagcgtt
    42001 ctgttgagcc atggaaaata aacagcaata cgatcgaacc tagcgctagt gtgataatcc
    42061 cattggaata gcctagtcga tccccacgat cttgatacat gtgaggcatg aatttatctt
    42121 ttgccagatt gtatgcaaga actggaaaag cagaaaaacc ggtgttagca gcaactgcta
    42181 aaatcagcgc tgtagcaaat tgaaggacgt aatacaaaat accatgtcca aagacagctt
    42241 taccaatttg agacaaaaca gtgacttctt tttcaggaac gatcccatac caatagttga
    42301 taaatgtgat ccccacgaag aagaaaccta agatcaatgc catcatcgtc aatgtagcag
    42361 cggcattttt tgctcttggc ttcttaaaga atggtacagc attactaatc gcctccaccc
    42421 cagttaaaga ggaagaacca gaagaaaatg ctcgtaaaat caaagcgata gaaacacccg
    42481 gcacagccgc tcctggaagt gcagtagctt gcaatggcac agcacctgtc acgatattat
    42541 ataaaccaac aacgatcagt aacgagatca caaggataaa tgtgtacacc ggaaataata
    42601 ggaacgatgc agattccctc agcccacgaa gattcagcat catcagtagc aaaacgattg
    42661 tcacagaaat agcgacttga tgaccgtaga gtgctggcac cgctgatgtg atcgcttcag
    42721 cacctgctga gacagatact gctactgtca acatgtagtc gatcaataaa gagccaccgg
    42781 aaatcaagcc agcattcttt ccaagattct cactgctgac gacatatgct cccccgccat
    42841 gaggataagc atggatgatt tgacgatatg atagcgttaa tgaaatcaat aaaataataa
    42901 caaaagcggc aatgggtagc gaataccaga tggctgcagc agacaaagcg actaaaacga
    42961 caacgatttg ttccgtacca tatgcaatag atgataacgc atcagacgac aataatgcca
    43021 aagcagcaaa tcgggttaat ttgtgctcat cattttcagc agattttaaa ggcttaccta
    43081 ctaacaacct ttttaagtaa tccaatttct aaactccttt acacacaatt ttacttcaag
    43141 ttttatagtc gaaaatttat agtaaacaag atgttatagt acgctcactg accttaaaag
    43201 tcaataaact ttaagaaaat aattggaaaa aaacagaaaa atatcaagcg ctttttcaaa
    43261 cgctttaata cagcaaaaat ctaacatttt tttatgggct ttttctttga attctataat
    43321 aaaaggatag aacttgactt agaaaatcaa gttctatcct ttttgatatc ttaacttttg
    43381 gaagagagat gtaaggctgc tttctaccca tcattcttac tactcgaagc atagcgcagt
    43441 tgtcacaacc tcttgataaa cggtgtttaa aagcttgcat ctgcggtaat aagcccaacc
    43501 aaattaaaaa cataagaagc tgtttttatt tggtcgttct tattacttga tgcagagcag
    43561 cttttttaca acctcttggt acacggtgtt caatcagctg accctcggta ttagctcaaa
    43621 aaaacggcaa aatatgagga actatttttc catttttttc tctaatactc aggtcaaaac
    43681 actgatttca caacctcttt ttttaatgaa aaatatggct ctcatgcgcc gcttctttga
    43741 atttcgtcat tttttcttcg atgaacggat agattgccgt tcctacgatt ccaaagacaa
    43801 caaacaaacc gatcatgatc cagattgctt tcgtagcatt tggccaataa attcctcctg
    43861 cagattcccg taacaagttt accgcatgag taaatggcaa gaacggattg atcatttgga
    43921 agaatttccc tgagacttgg atcggatagt tcccgcctcc gccagagatc gacagtacta
    43981 agatgataat agcgatccct ttaccgacat tatcgaataa tgcagccaag acatagacga
    44041 tcatcatgaa cgctaaggcg atcaagacag caaatgacac gctgtagacc ggattttgga
    44101 catctactcc tagtaagaaa atattcccta atgtaacgat caagctttgc gcaatcgcca
    44161 ttgtcaaaaa tgtcagcata cgagcaacga attgttctcg tttcgtaaaa ttgtttcggt
    44221 ctttctttcc taaatagtat tctgtcgtag caacacttga aagaagaaca gcgccgaccc
    44281 acagacatag tgcagtataa aacggggtgc tagctgaacc gttatttgcg attggataaa
    44341 gcgcattggt ttgtagctca acaggttgtg taaagaagtc actttctttt gttgcgtcta
    44401 gtttcaataa tttgatgact tcgcctagat cgacttcttg ttcgcctttt cggatcgcgt
    44461 ctgctgcttt ttggatacca gctttgatat ttggccagtc attgttgatc aagtccgcag
    44521 cagcgttcac agctgtttcg acttctggca ttttttcatt tactgttgtc aatgtaccgg
    44581 tgatttcatt ttttagtgct ggccaatcat tttggataaa atcagcggcc aatcctaatt
    44641 tttgctgaat gatcggtaat tcattgttgt ataggtctgc cccttcattg atgccattga
    44701 cgatggtatc catatttcca ttcagcattg tattggcatc atggatttct tgtccaattg
    44761 caggcatttc tttttgatat ttttctaaaa ttgccactgc attcgttaca gttgctttcg
    44821 ttgagctgag aagcgaatca aagtcgatct ggtccgcttt gctcagtgcg ccttgtgcag
    44881 tcccaatcgt atcgatgact tggtctagta gagaagtgac agtggaactg atttgacctg
    44941 tattgatacc agcaatgatg ccattgatat tattaacaac ggcatcaatt tgatctagca
    45001 attcatcaat agccttcgtg tcttcatttt gaacagctgt gttcaaagca gataggcggt
    45061 tttgcagatt gcttagcaag ggttttaatt gatttagctg atcaataatt ggctgtaatt
    45121 tcgtatttcc agcacttgtt tgcaatttag tcaggaactc gaccagttta tcgattgcat
    45181 tttgttgttg agatattccg tcaataaagc cgttcaatac attgctgatg tttttctttt
    45241 catcatcagt cagcttacca tcttcaatag ctgttcttac agtatctatt aacgaacctg
    45301 tcgttgttgc cacttgtcca attgacttca atgtaacatc tacactgttt gtgatgcttg
    45361 gtatcgcatc tttcagctga gaggcacctg attttgtttg gtttgctaga tcacttgcct
    45421 gattttctaa atttttcaca tctggcaaga tatcttgtac ttgttggatg atcgtcaagc
    45481 cttgttttgc ttcatcgatt ccttggttca ttgtctcttg gatcgaagca aaatcgccgt
    45541 cgatctctgc tagttgctta ccggcatttt ggatctctgg gattttttct tgcaaatcaa
    45601 ggatgatttt tgcttgatct ttcaactcag gcattttgtc attcaatgca acgacttttt
    45661 cacccatctc atctacttta gggatgtaat ccacgaattc atttgcttta ttcagttttt
    45721 ccttgatttc aggtagttga ctttgtaatt cgaggacttg ttttgtgtag ccgtcgatcg
    45781 tgtccaaatt ttcatctgtt gataaaatca tatctttaac tttattgata cttactaggt
    45841 tcgtatcgat gtcgtaacct atctcattga atactttcaa taaagtcgaa ctggctgttt
    45901 tgataaattc atttgtgatt tgcgattgga tcgaacttgc tcctttatcg gtgattttcg
    45961 gtgcaatcgc attgattttt tcatttaccg tatattcgat ttttggtttc ttgatatcac
    46021 cactagtaaa actcagcaga tcttctgaga aatcctttgg caagtaaata cctgcgtaat
    46081 actttccgga acggactccg tcttctaatt gttcttttga atcaacgaat tgccatccta
    46141 attgtttatt tttatgaagc gattcgatga cttgctcccc aatggccact tctttacctt
    46201 gaaactctgc tggcttgtct gcactgtaaa cagcaattgg cagctctcca gtatttccat
    46261 atggatccca taatgctttg atgttaaacc acgcatatag cgaagggatg atcatgatcg
    46321 cgatgatcag aagtgttgca atcgggtttt tgaaaatccg tttccagtca agcaaatata
    46381 atcgaagcgt atttttgatt gactgcatag ttctccccct tcttcttttt tcagctgaat
    46441 tactttacca tgtatttcat acttttttca atcggctttt ttcaaaaaag tcgaattttt
    46501 ggacatatca cggcaattgt ctcattgtaa tctcttacat attgagaaat aattaaggat
    46561 tacagccttt actttatcat gaaataaaag ggaaagataa ttttatgcca aagtttttat
    46621 ctattatacg tatacgcaaa aaagctaaaa cattttttgt ctttgcccct acaaaaatgt
    46681 ttcagctttt ttcgtttttt attcctgtat ttcttatttt tcgaatgaca ggatgatttt
    46741 cccagcagga ggatgtcctt caactagctg atgtgcttgg atgacatgct taagtgaagc
    46801 agacagattc attgaaataa aaacatgaaa ggctcctttt tggtaggctt tgattatgcc
    46861 agcaaaggct tctgcatcta gatattcctt tcttggaaca aacgattcat aaaagccttc
    46921 ctttttttgc cgcaaatcta gatctgggag gtcatttaat gcaacatacc tgccgcctgg
    46981 tttcatgatc tggatgccgg tttctccttt gatacttcct ttcgtcgcat cgattacaag
    47041 gtctgcctga tcagcaaatt gcagacctgg atttgtttta tcataagcag caaatgctga
    47101 agctcctaat ttttttacct tttcctcatt tttactggat gcactggtta agattcggat
    47161 tcccttttca tggagaagct ggatcagact tgacccgacc gcacctgatg ctcctagtat
    47221 cattacagta tctgtcggct ggatttccgt caaatggttg attagattat aagccgtaat
    47281 tcctggcgtg accattccgg ctgcttcttc ccaactcatt ttatctggaa tttttgctac
    47341 ttttgctgat ggcaacacga cttcttctcc gtaagtcccg ccgactgcat ggacagctac
    47401 tcgatcacct acatgaacag ttgtgacatc tgaaccaatc tctgtcacaa taccagcgcc
    47461 atcattacct ggtacataag ggaatttcaa tgttcgtatc tctttcattt ctcctaaacg
    47521 caaggcaaca tcataaggat tcacactgaa tgcttttatt tctacacgaa catgtccaga
    47581 accgacttct ctgggatgag catcgatttc ttcaaaaaca tcttgtgctt ctccgtaacc
    47641 gttaattgca aatcttcgca tggtcaccat ctcccttttt ctttaagttt acgtttatgc
    47701 ctttattttg acaaaaaaaa tgtcttttcg ttttggcgca tgattcaaaa caaaatagtt
    47761 gtcttttttc gcgtaacgtg ttaaattatt tatctaaaag cgtaaagatt acgatttacg
    47821 ctaataaaaa ataattggag gaatttgttg atggctaaaa aatcaaagat tgccaaagca
    47881 aaaaaacagc aagaaacgat tgaacgctat gctgctttac gccaggaatt aaaagcgcaa
    47941 aaagattacg aaggcttgcg taaacttcct ttagatgctc gtccagaacg attgaaaaat
    48001 cgtgatttaa tcgatggacg ccctcgcgga tacatgcgta aattcgggat gtcccgtata
    48061 aaattccgtg aattagcaca taaaggtttg attccaggag taaaaaaagc cagctggtaa
    48121 tccaagtctg gctgtaatgt aaaggaggag ggcgcatgga ccactccttg gatcatcaaa
    48181 aaagatacgt tcgcttcttc tagcagaaat gctggtacta gaagcagacg tatctttttt
    48241 tacgcacagc tggtcctttt ttatatgtaa aggctgcatg gttacttcgc tatatttatt
    48301 tagtaacagg agtcagtgct tcgataccca cactaatttt caatgctcgg tcaacttctg
    48361 ccataatggg tcgatccaaa tggcagacct tttctttcaa tcttgattta tcgatcgttc
    48421 tgatctgctc caacaggata acggagtctc tttctattcc tgttccagta gctttgatcc
    48481 caatgtgagt cggaagctta ggtttagcca tttttgccgt tatggccgca acaatgatcg
    48541 tcgggctaaa atggttacct agattatttt gaataaccaa aactgggcgt gttccgcctt
    48601 gctccgatcc cacgactgga gaaagatcag caaaataaat atctcctctt tttaccatag
    48661 gatcctcctt attcttgata ttctcttggt atccgctgtc ctaatccgca agccacttca
    48721 tagtggatcg tacctaattg atctgctata tcctgcatag tgatttcttt gtctgcattt
    48781 ttgccgataa gcgtcacttt tgttccgaca ggaaattctt gaggcaatcg gatcatcagt
    48841 tgatccatac atacacgccc aatcgtttca cagtagttcc cttctaccaa tagagaaaaa
    48901 ccctgcatct ttcgtggcca gccatctgca taaccgatcg gtatcgttcc gatccactct
    48961 gcttcaggag tgatatatgt ttctccatag ccgattcctt cacctgctgg gagttttttg
    49021 acttgaatca attcagaaac cagctcaagt gctggctgca atggataaac ttccggtagt
    49081 gcgtgcccag agggattaag tccgtacatt gctactccat aacgaatcat attgccgatt
    49141 gtttcatcat gccataaagc cgttgcacta ttgctgacat gtacatatcg aggaagttcg
    49201 ggaagttttt tcagcacttc tataaagcgt tctttttgca gattccaata tgtatcatct
    49261 gcctgatctg ctgtagaaaa atgggtaaat aaaccttccc attctaatac acgactttct
    49321 ttgatgaatg cagctgcctc ttttacttct tcaggtgtac aaaatccgat acgacccatc
    49381 ccagtgtctg cttttagatg aatcgaaaga ggcgtctcta gcttactttt ctctaaaaca
    49441 gcagctgctg cttgcaacca ttcccgtgta cctgcagtta ctgataaatc atgagcaaca
    49501 gctacagtca gtgcgtcgta aggaaccata ttcaagataa ggatgggctg agtaaatcca
    49561 gcttctctca actcgactcc ttcatctaaa tttgaaacac aaaagcccga agctccacct
    49621 gcaactgctg cctcagctgt ttcaatagct ccatgaccgt atccgttcgc ttttactact
    49681 gcaaacaatt cttttccttg cggaagtctt gcaacttcat ttgctacatt ttcagttata
    49741 gcttttttat gtatcactgc tttcgttggg cggtgccaag cgacaaccat cgtttttcct
    49801 tctttctagt cttccaaaat aatttgtgca aaagctgttt catttgtatg agtaatactg
    49861 acccatacat tgccttcatg aggtgaacgc gtcacaacag gctgaccatt ttcatttttt
    49921 aaaatctcga catcttgaaa ggtaactttt ccgatcccag ttccccaagc tttagagaaa
    49981 gcctctttgc aagcgtaacg cccgcctaaa aattctactt ggcgatgaaa aggcaaggac
    50041 tggaatagtt tcatttcatc agaggtcaag attcgtgcaa tgaattttgg tttttcttcg
    50101 atcagccgag tgatcctagg aagttcgact gcatcgatcc caattccttt tatcatatgc
    50161 ttttctcttt tcaataaatt tagaacactc tcattctacc aaattataac cattttgcca
    50221 caaataagtc cacaaataac aaaaatgaaa gtttctctta aaaaagagaa actttcattt
    50281 tatgtttaat cgttatttga acggattaca aatccgcgtt ttttatcatt gtgacgttta
    50341 ccgccatctt tttggctgtt attatttttg ctataaccgc cttttgattt gtttccacga
    50401 taattaccgc cgtcacgacg attacggttg tttccgccac cacgtttgcc atttttattg
    50461 tatccttttt tctgcattgg caatgggcgt tctggtgtga tcttcactgg cactgcatca
    50521 gctggatctt ttgctgtcgt ttttagcaaa agtgcaacta aatcttgtgc agaatattct
    50581 tctaataatt tatctgctgt ttgcaggtat ttatctagac cattttctgc aagttttgtt
    50641 tcgatttgtt caactgcagc acctaattga cctttgaatg cttctttttc ggttggtgga
    50701 cgtaaagtcg tcatccgttt tttcgtcaag ttttcaatca catgcaagta gtccatttca
    50761 tttggtgtaa cgaaagtcac agacataccg ccttttccgg cacggcctgt acgtccgata
    50821 cggtgaacgt agctttctgg atcttgaggg atatcatagt tgtacacatg agtgacacct
    50881 gaaatatcta atccacgtgc agcaacgtct gtcgctacaa ggatatctaa atgaccgctc
    50941 ttaaatgaac gcaatacgct cattcgtttt tgttgagaca aatcgccatg gattccttct
    51001 gcacggtaac cgcgtgcttc taatccgcgt gccaattcgt ctacacggcg ttttgtacgt
    51061 ccgaagacga ttgttaattc aggtgtttga acatcaaaca agcgagtcat gatatcgaat
    51121 ttttcgtatt cttttgcgcg gacatagtat tgatcgatca agtcagctgt catttctttc
    51181 gctttgatct tcacatgatg tggatttttc atgaatttaa cgccaatatt cttgatagct
    51241 ggcggcatcg ttgctgagaa aagtaatgtt tgtctttgat caggaacttg tgaaatgatt
    51301 ttttcgatat cttccaagaa gcccatattc aacatttcat ctgcttcatc caaaactaat
    51361 gtttgaacgg ttccaagttt caatgtgtgg cggttaatgt ggtcaagcat acgtcctggt
    51421 gtacccacaa cgatatgtgg acgatctttc aagccacgga tctgacggcc aatatctgct
    51481 ccgccgtaaa cggcttgtac acgtactttt ttgtcgcgcc ctaaacgata taattcttct
    51541 tgtgtttgga tagctaattc acgtgttggt gcgataacta aaccttgtaa ttcgtgacga
    51601 tctggatcga ttttttctaa cattggtaat ccaaatgcag ctgttttccc tgtacctgtc
    51661 tgtgcttgtc caataacgtc ttttccagct aatgctagtg gaatcgtttc tgcttggata
    51721 ggcgttgctt cttcaaaacc agcgcgttcg actgatttca acaattctgg tgataattct
    51781 agttctttaa atttcaaatg aatcctccta tatggttgtt aactgaggat gtagcttttt
    51841 tgctaccccg tcgatttgtt cggtcaaagt gtattgtttc tttttgacag tggccacgca
    51901 cgtttttatc cattcttagc ccctgtggca ccaccgaaaa atcactttgc gaagtttagg
    51961 cattagaaaa gccaaacggg tcgcgttcag ctttatcatt attatgacat ctggcttcaa
    52021 caagttgttt ctatggcaaa accagtccaa tttctcttca tctttcggca ataggttatt
    52081 ctaacatagt ttttcgtttt ctgcaagttt acagattttt atttcatttg ctgaagttgt
    52141 tctaccactt caaataagcc catgctgttg ctgcctttta gcataatact atctgttgct
    52201 tcaagatttt gtttgatagc atcaatcaat ttattctttt ctttttcaaa atagaacaca
    52261 ggtagttggg ggtatttttg gttcaattct tcttttagtg catgcatttg ttccccataa
    52321 aggaaaatca tggcgaattt ctcaggatcg atatgttcac tcatttgcgc atgcatattt
    52381 aaagactctt tccctaattc cagcatatct gctaacacag cgattctgcg tccttttgtt
    52441 ggcagatttt taaaagtatc taataccagt cccatggctg ttggatttgc attgtagaca
    52501 tcgctcaaaa tagctgcacc atttcctgcc gttagccatt cggttctgtt ttgagtgata
    52561 gaaacatggg cgagcccttg gcgtatctca ggtatggaca gtccaaagaa gcgcccgatc
    52621 gtatacgcaa tcaaagcatt cgttacatta taacttccag gcagcgggat cgtaaatgtt
    52681 tctccttcta ctacaaaatc ggtttgtgct tttccttttg ctgtcacttc cgcatgaaca
    52741 tcgccatttc caataccgaa tgttgtgact gtttgtacta atgttgccgt taaaggtgtc
    52801 agcaacggtt catctgcggg cacaacgagt aatccgtctg atacaagtcc gtcagtgatt
    52861 tccattttgg ctttagcgat tccttctcgc gacccaagat tttctatgtg tgcttcaccg
    52921 atcattgtga ttgctgcagc ttttggttga gccataagtg aaagttccgt gatttcattg
    52981 gcgtgatcca tgcccatttc caaaacgatc ttttctgtat cttggggcat gtgcaaaatc
    53041 gtgtaaggaa gaccaatatt attgttgtaa tttccttgcg tcttatatgt tttgtatttt
    53101 tcagacaaga cagacgcggt tagatctttc gtggtggttt taccattgct tcctgtaatt
    53161 gcaacgactt ctgcgcctac tttttccaag taataggttg ccaattgctg catggctttc
    53221 aaaggatctg ctacaggaat aactggaaaa ttttttggag cttgttcaac aggcaagctc
    53281 caaaatgaag cagatgctcc gtttgtgatc gcttgctgga taaattcatg tccatctcgc
    53341 gtaccagcaa gaggaacgaa taaacttcct tcggttatct tccggctgtc aaattcgaca
    53401 ttggtaatct ttgtcagtgc agagcattcg ctgcccacgg cttgtgcaat ttcttgtatt
    53461 gttagattca ttgagatctc tcctaaaaaa ggttgtgaaa aaagcgtttg gcctaaacgg
    53521 cgggttcgca tgactctcac ttccaagtaa atttctctat tcgtatttag aagctagaat
    53581 gaacgcggtt acgcaatcac tccagcctct tctttatttc tctttattca tttttttcaa
    53641 aaaaagattg acgctgatgg tatcgattca ttcctaactg gatcagttct tcaatcaaat
    53701 caccgtattt caagcccata ttttcccata agagtgggta catactgaac tccgtaaatc
    53761 ctggcataga gtttaattca ttcaggaata attcattttt atttgtcaaa aagaaatcgc
    53821 accggctcaa tccgcttcca cctaacatcg tgtaagctaa cttcgcgtac tcttgcgctt
    53881 tttgataaac ttcttctggc acttcggctg gaatctgcat ttcgatttta ttattgatat
    53941 attttgcttc ataatcatag aatgctacgt cttttacgac ttcaccaggc aaagtcgtcc
    54001 gaacgtcttc atttcctaat acagcaactt cgatttcgcg cgcttcaatt ccttgttcaa
    54061 cgattgctcg agaatcatac tgataggctg ttgctaaagc attttgcagc tcttctcggt
    54121 tttctgcttt tgtaatgccg acactagaac ccatattcgc cggtttgaca aacatcggat
    54181 aaagcaaaga accttcacat tgatcaaata cttttttagg attttctttc cattgattct
    54241 taagtactgg tacataagga acttgcggca caccagcagc ttgtaaaata tacttggtca
    54301 tgattttatc catggcacat gcactggtca atacgcctgc gccgacataa ggcatattca
    54361 atgtctctaa gaagccttgg atcgttccat cttccccgtt tggcccatgt aaaactggaa
    54421 aaacgatggc tccttcttct ttgatttcgc ccggattgat cacttttcct gtaaagcctt
    54481 cctctgtctg tccacttggg tcccatgaaa gatgcaagac atctttgctg gcaggttttt
    54541 ctgttagtaa tggacctttg acccattgtc cttcttttgt aataaatacg agttgaactt
    54601 ggtaataatt ataataaatg gcatttaaaa ctgaaaatgc ggaaagaatg gacacttcat
    54661 gctctgcgct gcgtccgcca tatagtaaag taatcttcaa aagattgtcc tcctgtattc
    54721 taaatcgttc agtgatttac tctcatattt tagcacaaaa caaaaataaa cgaataactt
    54781 atttataaaa aaacgagcca gcttctaaaa gaacgagcgt catttatttt ttgtcagcta
    54841 gattatttgt cccgtttttt ataaaaagtt atgataaaga aaaggaggga tgatgatggc
    54901 gcaaacaaat ccacgcagcc gcttggcaga aatcattcga gtgttcatca attacaatgt
    54961 agtgccaaac tttgtacagc aaaaaaatcc cgaacaggta aaaaaagcct ttgaagaact
    55021 aggccccacc ttcatcaaga taggacagat gctttctgtt cgagaagatt tgctttcttc
    55081 tgccttcact caaacattca aaacattaca agatagtgta cctagcgaca cattttcaac
    55141 ggtcaaaaag acgatcgaaa cagaactttc tctttcatta tcagatatat tcgatgattt
    55201 ttcaaaatcg ccttttgcct ctgcttctat ggggcaggct catcgtgcca agctgaagaa
    55261 cggtgacgcg gtagtcgtta agatccaaca ccctaacatc gccgaagaga tccgacttga
    55321 tcttcaacta tttgaacgag cgatcccttt gatcaaatac attcctgaaa ctagcgtagt
    55381 tgatctaaaa ggggtactcc aagaagtaaa acgttccttg atcaatgaaa tggacttctt
    55441 aaaagaaagt caaaatggcg aacaattcta tcaaaagaac aatggctgga aagagatccg
    55501 cagccccaag atttatgacg ctttttgttc gaaaaaagtg atcgtgatgg aagaaatgtc
    55561 tggtaaaaac ttgaatcatt taatgaacgc cgaaaacaaa actgaaactt ttatcactgg
    55621 aattcaaaac aagcagctga aacaagaagt tgcaaagctt ttagttgaga actttatgaa
    55681 acaggtgttt gatgatggtt ttttccatgc tgatcctcat ccagggaatc tgctattcca
    55741 tgtactaaca aaagaagaac agacacaagc tagccgaaaa actgaaacag tccatgaaaa
    55801 agagttcggt tcctttgctt ttcgagcctc cacttctgca gaagaccctg ttgcgccata
    55861 taccatcaac tacatcgatt ttggtatgat ggggcatctt tctgcaggac ttaggcaaaa
    55921 actcacacaa gccgttttag cgctgtatac gaaagatgct tatcgaatcg aaaaagcggt
    55981 ccttcgactt tgtcagcaag aaggctcgtt tgatgaaagt cggttccacc aagaactgac
    56041 aagttttctt gaacagtatt atgattctcc tattgatgaa atcaacttgc aagaagtatt
    56101 cacacatgtc gtgacgattt gtcatcaaaa taatctgcag ttcgatcgtg atatcacgct
    56161 tttgctgaaa gcatttggga cattagaagg ggtgatccgc gtcctcgatc ctgaagtttc
    56221 cctcatggaa gtcgcttcgc cttttgctca acattatttt ctcactcatc tggatgtaga
    56281 agatacattg aaacaaagcg gattagactt attggaaggg atgaaggctg cgcctaagat
    56341 ccctcaacag ctccaccatc tattagaaat gtggacttcg ggacaaggaa aagtcaattt
    56401 ggaattgaaa aagcaagaca aattattgag caggatcgaa agtatgatca atcggcttgt
    56461 tttcggtatg attttagctg cattgatcgt agggtcctcc ctgcttgtcc aagcagcacc
    56521 tgtagaaaac gcagaggtcg tttccttatt agggatattt acgtatgcta ttgccgcatt
    56581 cgtcattatt tttttggcaa tcgatgcatt gatccaaatg tataagaaga ggaaaaagta
    56641 gaaaaaggtt gtgacagaaa gaatcagctt caagaaataa gaaggggttt tacataaatt
    56701 gtttctcaaa tttttgtgaa aattcggctt atttttcgaa gaggctactt ctgaaacacc
    56761 gagaataaaa aatgtgaaaa agctgttctg cacccagtaa taagaacaat caaaaaggga
    56821 acatggaaca catttatcct gtttccctgt tccctttttg atgtattcga gcataaataa
    56881 aataccttcg ttacctacta tcttctgtac ttggtaaacc ttagtcaatt gcctttttta
    56941 acgcttgacg aaaagacata tgatctttct ccgtaaaggg ttttggaccc tttgtcctta
    57001 tgccagcttt tcttaacttt ccactatgat aacgttgttc tagaagcgtg ccgatattcg
    57061 cttcgtcaaa ttcttttcca gagtgatgga acactcgcac acccttcggc aatacaagtg
    57121 aagctaaacc ataatcttgc gtgatcaaaa agtcattctt cttaaccata ccgacgattt
    57181 tataatccgc actatcagct ccgcggtcta cgtagacgat ttggacatga ttagcgtatt
    57241 ctttggtcgt ataatggtcc acactggtca cgataaccac gtcaagacca taggattctc
    57301 ctaattttat gacatcttct tttactggag aaccatctcc atcaatgaat agcctcattc
    57361 tttttcgcct tcttccataa atgataaaca gttactaatc cgctcagtaa aagaccaaca
    57421 ttgatccatt ttgccaagat aagcagttgt acactttcta tcggcactga ttcatggttc
    57481 agcgtgatca ataatagatg gattggctct cccatttctt ttgctaaaac aaacaaaagc
    57541 gtcatcaata aaaactggtg gatcacactc cctttatttt gagtagtata cttgacaacg
    57601 atccggcgat ccattggaat agctgtatca gtttgataat attccttatt tagccagctg
    57661 cctgttacta cgtgccagaa actaagcaga aagacaccta tcgcaaaacc atttggaatc
    57721 aagaggacca ataaagaaga actcagtcca agcaattgca tagccaatgg agaccatgtt
    57781 aaacgtttca aaatccatgg tccgactatt tttgctgcaa tcaagcctgc tgcataaggc
    57841 aaatacataa aaatcgaaag tttttctaat ccgtacagta tgccaacata aaaactgcta
    57901 aataaaaaaa ggaaattccc tagcattcca tttagaaaag taaacagatt tagccagctt
    57961 ggcaacttcc attccttctt ctggtgtcct aaaacaaaca cgaacaagat gaacagcagc
    58021 aagaaaccat aaatagcaag atcaaaaagc ctttcatcga ataacaatcg ggcatttctc
    58081 aaaagaaaca ataagccaaa gaatatcaaa aataaaacaa gctctcgcgt agctaccaga
    58141 tttcttttga tgtctttgaa atccagttcg taacgcggat aatgactaac cgtatggtag
    58201 gccatgacgt aaaaaagtgt ataaatagta aagcttgctg caatctgagt cggtgcaggc
    58261 aactcaatcg cttgatacag gagaaatagc cagatagctg caaataagta ctttttccca
    58321 tccatattga taaaaccttg cgtcttttcg tgaaaattga ccgtggtatt agcaggtaca
    58381 agccatgctg cactcagccc tagcaaaaca cttgaaaaat aaaataaagg aaaataaaat
    58441 acgcctaata ttccaagcaa tgaaccagca ccaccaatca gcagagcgat catcagcaac
    58501 gtgtaactat ctagaccaaa tcgtagactg ttgactaaaa atagtccagt catccgaaag
    58561 gcataaaaca gcgtaaatgg caaaatcgtc agccaattat gcgagtctgt tagtcccaaa
    58621 aataaaagat aaggaataaa aataaccgtg ttacttaaga taaagggcgt tgtttgtgta
    58681 aaattacgcc caaatcgttt gagtttttcg aacatgacaa tggctcctta aacagtctta
    58741 taactaatct gctcttcgat tttagcatta aaggggaaat aaaccaattt tacagaatat
    58801 attcttgtaa aaaaaaatac agaaagaagc ataagcttct ttctgcattt ttacggtact
    58861 tgattactca agcagacaag cccttctcca caaaagtctt gcccgacacc cctgcatccg
    58921 gcaggtgata aagatagaaa tcaattatcc tgtgcctcca aaatcaatta ggagctctgt
    58981 aaaccagagt ccttctcgac ccgtcgcata aatttacttt aacacagttc ctatgtttat
    59041 gcgagagctt ttttcatttt ataatcgacc atttgaaatc cttgaccgat atctgaaata
    59101 agttcttctg taatctcaaa tccttcatgc ttataaacct cgattgcttg atgattcccc
    59161 tgattcactc gcaaaaagat ctctctttcc tttgtttgcg cagcaagttc ttcataccag
    59221 cgaaaaactg aagaagtcaa tccttgacca cgtactttat ttagcaaata taatttactt
    59281 aaataaattt ttccatttat ctcttcataa gcagtgtaac cgacagtctt ttcttcgctt
    59341 tttaataaaa agtaggacac accttctgtc agttcttttt gaatctgttt ttttgactga
    59401 taagttttca gcatgtaatc cacttgtttt tgaccaatga tcggcacaaa aacttccggc
    59461 catacttctt gtatgactgc catcagctca tccagttcat tttctgttac ttttattttt
    59521 tccatacacc cacgtccttt tttacattat aaatgacctt gcgcttgatg gaataaaaag
    59581 agcatgatca tgctatacac acccaccgac cacggtttca agaaaatcac aatctgcata
    59641 aattccctaa aagataattt cgtcaaacca gctactagac agatcaagtc gtctggcgca
    59701 aagggaagca gcaaggtaac aactagtact tttttaatat ctttcgtttt tcttgtcagc
    59761 aattcatcaa atttctgata cttttttggc gataaaataa gctggacaaa ggctacgcca
    59821 tagtaacgga caagcaaaaa tccgattgct tccccaatca ctaggccaat acaactatat
    59881 aaaagcccaa atccattgcc aaacatcagc atgcccgcta ctgaggagat ccctcccggc
    59941 aaaataggaa tgatcacttg gatcacttgc aacagaacaa atatcatcac tgcatacttg
    60001 ccaaattgct ggataaacgc ttgcaaagac gtgctcgatc gaaaaatccc atgatgataa
    60061 gcataaagaa tcaacacaaa gaaaaaaaac agaccaataa tgggcatgat ctgaagacat
    60121 ttttgtaaaa ctgatccttt tttagacatc tatattcccc acatcttttt tcatttatta
    60181 aaactgcctc ctt
//
LOCUS       DARBET010000009        57190 bp    DNA     linear   BCT 07-NOV-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4027
            SAMEA114569841-rid20112183.denovo.009, whole genome shotgun
            sequence.
ACCESSION   DARBET010000009 DARBET010000000
VERSION     DARBET010000009.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA114569841
            Sequence Read Archive: ERR12203666
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 57190)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 57190)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001972618.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001972618.1
            Genome Coverage       :: 132x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/07/2023 13:18:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,041
            CDSs (total)                      :: 2,975
            Genes (coding)                    :: 2,908
            CDSs (with protein)               :: 2,908
            Genes (RNA)                       :: 66
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 67
            CDSs (without protein)            :: 67
            Pseudo Genes (ambiguous residues) :: 0 of 67
            Pseudo Genes (frameshifted)       :: 26 of 67
            Pseudo Genes (incomplete)         :: 28 of 67
            Pseudo Genes (internal stop)      :: 20 of 67
            Pseudo Genes (multiple problems)  :: 6 of 67
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..57190
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA114569841-rid20112183.denovo.009"
                     /isolate="VRE4027"
                     /db_xref="taxon:1352"
                     /country="Denmark"
     gene            complement(<1..205)
                     /locus_tag="SCJ86_000653"
     CDS             complement(<1..205)
                     /locus_tag="SCJ86_000653"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YSIRK-targeted surface antigen transcriptional
                     regulator"
                     /protein_id="HEG1168239.1"
                     /translation="MFSDKEVLLKSLHVCLKLPIVVFNSEFSIIKEYRSDRTTYFFYD
                     YKKLLTDNIKKTKDFHFFTGDFNE"
     gene            899..1285
                     /locus_tag="SCJ86_000654"
     CDS             899..1285
                     /locus_tag="SCJ86_000654"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016922530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HEG1168240.1"
                     /translation="MSDNDERVNAQLIRFFEKVILNTANTYYKKQEEIQKHEQLDQLP
                     PYLFTKDTIKFKQPINVLNVTLLVEDIKLAKILPLLKEPEQIFLIEKFIFDKTDKEIG
                     KVLGITRQGATNLKHRLYKKIQKMLV"
     gene            1379..1570
                     /locus_tag="SCJ86_000655"
     CDS             1379..1570
                     /locus_tag="SCJ86_000655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HEG1168241.1"
                     /translation="MNSLKEDFILAKAGNEEAVEAILKRFSSLIHKQSWRTGKYDQDC
                     YQECMLAIYLAISKFEIKE"
     gene            2084..2416
                     /locus_tag="SCJ86_000656"
     CDS             2084..2416
                     /locus_tag="SCJ86_000656"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HEG1168242.1"
                     /translation="MRKTYDRKFKLKISQDILEKKVTTKKIAEEYNISRPTISRWVSE
                     YRRYGKNAFAGQGKRLPDKADFYILEQENKRLKEEDECSTNTKIDTDLQGSLKNFTMK
                     ESKSINVL"
     gene            2712..2867
                     /locus_tag="SCJ86_000657"
     CDS             2712..2867
                     /locus_tag="SCJ86_000657"
                     /inference="COORDINATES: protein motif:HMM:NF012870.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase family protein"
                     /protein_id="HEG1168243.1"
                     /translation="MPTVIHSDRGSQHRSFIYQELLNDHHITHSVSQTGTPVDNAVIE
                     AFHRSIK"
     gene            3143..4483
                     /locus_tag="SCJ86_000658"
                     /pseudo
     CDS             3143..4483
                     /locus_tag="SCJ86_000658"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303667.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ISL3 family transposase"
     gene            complement(4724..6340)
                     /locus_tag="SCJ86_000659"
     CDS             complement(4724..6340)
                     /locus_tag="SCJ86_000659"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002406986.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NusG domain II-containing protein"
                     /protein_id="HEG1168244.1"
                     /translation="MKKIPITILTGYLGSGKTILLNHILYGDHGKEIAVIVNDIGEVN
                     VDGVLVDKKGFKRTGEKLVSMPNGCICCTLREDLIVELDQLVQFSDLNQIVIEASGIS
                     KPIPIIQAIILAKNPLDIDLTQRLEIGAIIIVVDVKRFWDNFETGEKIQERKAEGNVV
                     EEEQDIRGLLIDQFECCNILLLNNSDLVSTKTIYKINGLIAKLQSNAKIIRTIKGEVD
                     LSKILNTNLFNIDETSYSVGWFKELELGYQNHIPETEEYGISSFIYRNRLLLDAQKFA
                     HLTDKNFPGNITRAKGFLWFAQHSDFSTIFETSGSYKSIESLNYWVASLPKIECQMII
                     QKNPELKEAWHPLFGERINKLVFIGMKMDQQCIKEQLEGCLTDSLNADNFRKISPFPW
                     KKSNMFSQISLMKFLRKAGVKIGDVIIIGILIITSFIPLFVFSIEKENETTEDAIKYA
                     IVRIDGKEMDRFDLDKINHKLVNYHPAKGQYNIVEIKDGRIRVKEDNSPDQIAVRTGW
                     ISKPGQTSICLPHKLVISIEKKESTDYYIY"
     gene            6458..6676
                     /locus_tag="SCJ86_000660"
     CDS             6458..6676
                     /locus_tag="SCJ86_000660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330794.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HEG1168245.1"
                     /translation="MSQYRHLTILERERIFLLHEEGFSISIIGNDIQHSPFTILHELR
                     RNKKKTIHLPKRKKIMLIEEKNADVITF"
     gene            6871..7332
                     /locus_tag="SCJ86_000661"
     CDS             6871..7332
                     /locus_tag="SCJ86_000661"
                     /inference="COORDINATES: protein motif:HMM:NF033563.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS30 family transposase"
                     /protein_id="HEG1168246.1"
                     /translation="MHHRGKTRHTKNHVESRGKICLTHSIHGRPKSADDRSDFGHWKA
                     DIVAGKTGGTCLVTLTDRKSCFLLAGKKVPRKKAAYVSEKMIELLSSIPNEAVKTITP
                     DRGKEFSNHFAVTEALNNMPFYFPDPHALGNNEQIKIRIDSFENTCQNRKR"
     gene            complement(7525..7764)
                     /locus_tag="SCJ86_000662"
     CDS             complement(7525..7764)
                     /locus_tag="SCJ86_000662"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304820.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HEG1168247.1"
                     /translation="MVRKVLLSMDSKNIDAYRRYRFAKQMRLNGSTLADIAATLHVSI
                     DTVHRVLKRDPDCCFIKVNTGRSRRLAKRHNSLAN"
     gene            complement(7788..9311)
                     /locus_tag="SCJ86_000663"
     CDS             complement(7788..9311)
                     /locus_tag="SCJ86_000663"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302413.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ABC transporter substrate-binding protein
                     AdcA"
                     /protein_id="HEG1168248.1"
                     /translation="MKKISVGLIGIAALGLLGACSSTNDAKVSNDKDGKLEIVTTFYP
                     MYDFTKNIVGDEANVDLMVPAGSEPHDYEPSAKDMAKAHDADVFVYHNENMESWVPKA
                     KESWKKAGPNVVEGTKDMILLPGSEEEDHDHGEEDHHHELDPHTWVSPKMAIKEVSNI
                     KDQLVKLYPKKAKVFETNAEKYLTKLKRLDADYTTSLKEAKQKSFVTQHAAFGYLALD
                     YGLKQVPIAGLSPEEEPSSGRLAELKEYVKKNKINYIYFEKNANDKIAKTLANEAGIK
                     LEVLNPLESLTKEQMDNGEDYVSVMEDNLKALEKTTMVAGEEVVPEKEAKDEKTVANG
                     YFKDADVKDRELSDYTGEWQSVYPLLKDGILDEVFDYKAKINKDMTAAEYKDYYTTGY
                     KTDIDTINIKDNTIDFVVNGEHHQYTYKYVGYKILNYEKGNRGVRFNFETDDAGAGRF
                     KYIQFSDHGIAPSKAEHFHIFFGGESQEKLYNELHNWPTFYPASLSKHEIAQEMMAH"
     gene            complement(9329..9451)
                     /locus_tag="SCJ86_000664"
     CDS             complement(9329..9451)
                     /locus_tag="SCJ86_000664"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302412.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative metal homeostasis protein"
                     /protein_id="HEG1168249.1"
                     /translation="MEKTDISSAYRRLKSPNIKTRKRALKIIKEHKRNKMKKLA"
     gene            complement(9481..10464)
                     /locus_tag="SCJ86_000665"
     CDS             complement(9481..10464)
                     /locus_tag="SCJ86_000665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002360310.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTP-binding protein"
                     /protein_id="HEG1168250.1"
                     /translation="MGIPVTIVSGFLGAGKTTLINQALQVSPYPREEIIIIENEFGEV
                     GIDHKLLVHSEERVLQLNNGCMCCSLRSDLLAILISLLETIEEHQQPISQIIIETTGI
                     ADPQPIIQTLLTAPQLRGRVYIDSLLTVIDSDSFELIEREPQALKQLVMADRIFVSRK
                     QNLNLSALKKLQREIKSINPLSDIRMFTHSETIQKKDFYNLKKFNHPLVFESDSSENH
                     YNGKYYHQHHQVHEFKSILLTSDQDIKKDFLLQWIDWLILTNQGSLYRFKGLIALQEQ
                     DFIVAMQEVNEQVNFQYTTRQKDQTTIILIGKMLDEERIKQSFDKLLAESK"
     gene            complement(10488..10637)
                     /gene="rpmG"
                     /locus_tag="SCJ86_000666"
     CDS             complement(10488..10637)
                     /gene="rpmG"
                     /locus_tag="SCJ86_000666"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002358586.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L33"
                     /protein_id="HEG1168251.1"
                     /translation="MRQNIILECVETGERLYLTSKNKRNNPERLELKKYSPKLRRRAI
                     FKEVK"
     gene            complement(10658..11035)
                     /locus_tag="SCJ86_000667"
     CDS             complement(10658..11035)
                     /locus_tag="SCJ86_000667"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016252202.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEG1168252.1"
                     /translation="MEIIYPPLVEEGLKYYLETTQQSLDKCTFYRSMVERGIITETGL
                     PTQQAIENGLVKDYYEDQGLSFDDFLRIYPIFEEYDEELFQCIDGYWEIPVDMKENLV
                     SQLESGELTFEDAQQIQAYLEDR"
     gene            complement(11067..11246)
                     /gene="rpmF"
                     /locus_tag="SCJ86_000668"
     CDS             complement(11067..11246)
                     /gene="rpmF"
                     /locus_tag="SCJ86_000668"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018345355.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L32"
                     /protein_id="HEG1168253.1"
                     /translation="MAVPARKTSKAKKRLRRTHQTVVKPEISFDEANGDYRRSHHVSL
                     KGYYNGKKVIKGASK"
     gene            complement(11261..11530)
                     /gene="rpsN"
                     /locus_tag="SCJ86_000669"
     CDS             complement(11261..11530)
                     /gene="rpsN"
                     /locus_tag="SCJ86_000669"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002382898.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S14"
                     /protein_id="HEG1168254.1"
                     /translation="MAKKSKIAKAKKQMAMIEKYADKRQELKAAGDRTALAKLPRDSN
                     PNRLRLRDQTDGRPRGYMRKFGMSRIKFRELAHQGLIPGVKKASW"
     gene            complement(12053..13819)
                     /locus_tag="SCJ86_000670"
     CDS             complement(12053..13819)
                     /locus_tag="SCJ86_000670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002371020.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEG1168255.1"
                     /translation="MQMIGGLTMKSFYKNKFALSDQGAEALTKASISSFFVYCINMVP
                     AFIIMMLIDELVLENTKPRWLYFAVSFVTLLFMYWLLDREYENLYNSTYKESAHLRVQ
                     IADDLSNLPLSYFSKHNLSDLSQTIMSDVEGIEHAMSHAIPKAGGMALFFPFISVMLL
                     VGNVKMGLAVILPTLFSFVLILLSKKSQTKANTKYYDTLRENSEEFQETIELQQEINS
                     FNLSKKVQDRLFKKMEESERIHLKVELSTFSVMALSSIFSYVSLAVVILVGVHLLLTG
                     EVTILYVVGYLLAAIKIKDSFDSMKEAVLEIFYLAPKIQRIRAMKETSIQEGSDSPLK
                     SFDIELRDVSFSYDNNTPILDHISFTAKQGEVTALVGASGSGKTSILKLVSRLYDYDE
                     GCILIDGYDIKRVSPASLFSKIAIVFQEVTLFNTSILENIRIGNSQASDEEVKKAARL
                     ANCEDFIEKLPDGYHTLVGENGSSLSGGERQRLSIARAFLKNAPILILDEITASLDAE
                     NEKRIQESLNRLIQDKTVLIISHRLKSIEKVNKIVVMDQGKLVDQGTHNELYRRSEIY
                     KNLIKKTKLSEKFVYEKEAQSR"
     gene            complement(13779..15532)
                     /locus_tag="SCJ86_000671"
                     /pseudo
     CDS             complement(13779..15532)
                     /locus_tag="SCJ86_000671"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398247.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
     gene            complement(15642..16211)
                     /locus_tag="SCJ86_000672"
     CDS             complement(15642..16211)
                     /locus_tag="SCJ86_000672"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297308.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MptD family putative ECF transporter S
                     component"
                     /protein_id="HEG1168256.1"
                     /translation="MKTKDYIFIGLATVISLVIYFFSIMVSSIGGAFGHSISPGIFGL
                     LSGIIFVYISYNYPRKGIFTIYTIVLLLFFTLMGGAYLPWFISSISMAILADIILSVF
                     GYDRAIPQVASWALMQLGSAAGQWIPIWFFTDRFRQDWIDKGQSAATMDAMIHYAVGI
                     WGIISVLVVASLSMIGVLIGRKVLKKYKK"
     gene            complement(16229..17644)
                     /locus_tag="SCJ86_000673"
     CDS             complement(16229..17644)
                     /locus_tag="SCJ86_000673"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297306.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="energy-coupling factor ABC transporter
                     ATP-binding protein"
                     /protein_id="HEG1168257.1"
                     /translation="MFEFNNVSFSYKDVKEQALVDLNFSIKKGSLTLITGSSGSGKST
                     LLKLMNGLIPRLYDGKLVGEILFENNSLQSLTQESISKNIGYVSQDPRGQFFTTNTTS
                     ELVFSMENFGFTKEHMDKRINELAELLDLSNILDKNIFSLSSGERQKISIGCSLSLNS
                     KLIILDEPSSNLDFKATKKLADLLCDLKNKGFTIVIAEHRFYYLKELIDQVLFVKNRT
                     VKTTTIQLLKTENNNALRKFDIFNSSIEVPYIEKVSADVLKVEEITYENILKDINFDV
                     KVGDVIGLVGKNGVGKTTLLRILMKIIRPTKGKIIENKKNLSSPFLVMQEMDYQFFTE
                     SVRSELALGNEIVSKDQQEKILKKMELYKMKDQLPFDLSGGEKQRLLVSIASLSKTNL
                     FLFDEPTSGLDYINMERIADLIKDLKEKGAVVIATHDPEFLYKTCNRIIYLKNGGIKK
                     DIKLRVEDSTLIEQIFNDITK"
     gene            complement(17655..18323)
                     /locus_tag="SCJ86_000674"
     CDS             complement(17655..18323)
                     /locus_tag="SCJ86_000674"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002360298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="energy-coupling factor transporter transmembrane
                     protein EcfT"
                     /protein_id="HEG1168258.1"
                     /translation="MSKINPLTLITLNIFFPVIIFLGKGNIFQVGCFIIATVLVLMFR
                     KIKTCIYFILIYSVLFLVMKIIDAIKIEFFTIFFATSLYIVFRMLPVVMISWLLVSVY
                     TSNELLSSLEKIHLPKKIMLSITVVIRFFPTYRSEIRMIKESLKMRNIRLSFFQPLQY
                     LEFLLVPVLIRATMIAEEMTANAITKGIESPVKRSSYYKTNMSLLDYIILFSVTIFFL
                     SILI"
     gene            complement(18453..19037)
                     /locus_tag="SCJ86_000675"
     CDS             complement(18453..19037)
                     /locus_tag="SCJ86_000675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002364863.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HEG1168259.1"
                     /translation="MESGLSHFEKEGFQQTKIRQLCLTAGVTTGAFYKHFTSKEALFE
                     EIIQPHINALINMYNEEFTKYLLNLENIDIEKSWRENFNELDPFINYIFENKRIFDLI
                     LFQSAGTKYQDIVEKITSFVTKKTVCCLDKLKTIGMINQNLVFNEEEIHFYIYSFYAT
                     FYDILKHSYSKEKTMLLTRRLYQFSVPGWIELLS"
     gene            complement(19368..19601)
                     /locus_tag="SCJ86_000676"
     CDS             complement(19368..19601)
                     /locus_tag="SCJ86_000676"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002382893.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-dependent repressor"
                     /protein_id="HEG1168260.1"
                     /translation="MVQIKTTTILIYLLAIQELSKRKKKLKNNDLAKVLSVSPASVSE
                     MLAKLRYEDYLDIGFQLTDKGKKFIDDYKKGFK"
     gene            complement(19616..20566)
                     /locus_tag="SCJ86_000677"
     CDS             complement(19616..20566)
                     /locus_tag="SCJ86_000677"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322077.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter substrate-binding protein"
                     /protein_id="HEG1168261.1"
                     /translation="MMRKWKAVLGSLGILIALFIFGACSINSKDKDKVASNEKLKVVV
                     TNSILADITENIAKDKIDLHSIVPIGKDPHEYEPLPEDVQKTSKADLIFYNGVNLETG
                     GNAWFTKLVKNANKEENKDYFAASDGIDVIYLEGQSEKGKEDPHAWLNLENGIIYAKN
                     IEKQLAEKDPDNKKFYKENLDKYIEKLDSLDKEAKSKFASIPNDKKMIVTSEGCFKYF
                     SKAYNVPSAYIWEINTEEEGTPDQIKHLVEKLRTTKVPSLFVESSVDDRPMKTVSKDT
                     NIPIYSTIFTDSIAEKGQDGDSYYAMMKWNLDKIAEGLSK"
     gene            complement(20563..21405)
                     /locus_tag="SCJ86_000678"
     CDS             complement(20563..21405)
                     /locus_tag="SCJ86_000678"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002364866.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter permease"
                     /protein_id="HEG1168262.1"
                     /translation="MLQNFINGLYDFHFLQNALITSVVIGIVSGTVGCFIILRGMSLM
                     GDAISHAVLPGVAISYILGINFFIGAIVFGLLASIIITFIKSNSVIKGDTAIGITFSS
                     FLALGVILIGVANSSTDLFHILFGNILAVQDIDKWITIGVSVLVLVIVVLFFKELLIT
                     SFDPLMAKAIGMNVSFYHYLLMVLLTLVAVTAMQSVGTILIVAMLITPAATAYLYANS
                     LKTMIFISATVGASSSLLGLFIGYSFNIAAGSSIVLTAAFLFVISFLISPKQKFLRKK
                     ERSL"
     gene            complement(21407..22126)
                     /locus_tag="SCJ86_000679"
     CDS             complement(21407..22126)
                     /locus_tag="SCJ86_000679"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016252206.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter ATP-binding protein"
                     /protein_id="HEG1168263.1"
                     /translation="MLEVKKLNVCYNDFLALNEISLKIQEGALTGIIGPNGAGKSTLL
                     KAMLNIIPHQGEVSINNEDINKKLVKIAYVEQKADIDFTFPIKVKECVSMGTYAGMKV
                     FQRIKNAEWQRVSKALEKVDMGKYSNHQIGELSGGQFQRVLLARCLAQNADFIFLDEP
                     FVGIDLVSERIIMDTLKKLKHQGKTVLIVHHDLSKVKKYFDNIILLNRNLIAHGSVES
                     VFNEENLKKAYGDTIFIGEGE"
     gene            22818..22976
                     /locus_tag="SCJ86_000680"
     CDS             22818..22976
                     /locus_tag="SCJ86_000680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320809.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HEG1168264.1"
                     /translation="MNKKQTCAFLGIDNNTLDKWIRIYNFPVIRVGGVVRFDVEDIKD
                     FMNTFKIT"
     gene            23048..24274
                     /locus_tag="SCJ86_000681"
     CDS             23048..24274
                     /locus_tag="SCJ86_000681"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HEG1168265.1"
                     /translation="MPTKLSNGKYKTNLRYPKRFKEITGIASEKYQKTFPNRQLAIKA
                     ENDMKKKIEKVLREENANSLELKGKITFKKFYESKWLPRYELGQTIRSNRPPSDITIS
                     NTKDIFRLHILPMFGEYAMNYLNINTEIISDELTKKSKEYANIKIIKGYVRSMFDIAE
                     ILNYIEFNRTTKIIQSITAPKKNALEEKRIQEGKQALSSKELTNWIEAVNDDFNNHLL
                     TFHDYTLFMITLYLGDRKSETYALQWKYIDFEKQTVRLKHTLDKYQRKKFTKGRKDTV
                     IQVPEVVMTLLSEWKSVQADQLLKLKIKQTLDQYLFTYTKPSGEVNCPVHADYLNYRI
                     NSIKRRHPDLVHLSPHKLRHTYATIARQGGADMNQISNALTHSDISTTKIYVNTPDIV
                     DKAVFEAFQRGLNKCD"
     gene            complement(24362..24754)
                     /gene="rpsI"
                     /locus_tag="SCJ86_000682"
     CDS             complement(24362..24754)
                     /gene="rpsI"
                     /locus_tag="SCJ86_000682"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002942223.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S9"
                     /protein_id="HEG1168266.1"
                     /translation="MAQVQYIGTGRRKNAVARVRLVPGTGKITVNKKDVEEYIPHADL
                     REVINQPFAVTETKGAYDVFVNVNGGGYAGQSGAIRHGIARALLEVDPDFRLALKRAG
                     LLTRDARMVERKKPGLKKARKASQFSKR"
     gene            complement(24770..25213)
                     /gene="rplM"
                     /locus_tag="SCJ86_000683"
     CDS             complement(24770..25213)
                     /gene="rplM"
                     /locus_tag="SCJ86_000683"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016250573.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L13"
                     /protein_id="HEG1168267.1"
                     /translation="MRTTYMAKPGEVERKWYVVDATDVPLGRLSTVVASVLRGKNKPT
                     FTPHLDTGDFVIVINADKVKLTGKKATDKIYYRHSNYPGGLKSITAGELRAKNSRRLI
                     ETSVKGMLPKNTLGRKQFTKLNVYGGAEHPHAAQQPEVLDITNLI"
     gene            complement(25732..26199)
                     /locus_tag="SCJ86_000684"
     CDS             complement(25732..26199)
                     /locus_tag="SCJ86_000684"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA starvation/stationary phase protection
                     protein"
                     /protein_id="HEG1168268.1"
                     /translation="MKFQQTKEVLNQLVADLSQMSVVVHQTHWYMRGPGFLTLHPMMD
                     EFMDDLNAQLDEVSERLITLDGSPYSTLREFADNTKIPDEIGRWDRTIPERLETLVAG
                     YRYLADLYQKGIEVSGEEGDDPTQDIFIEFKTATEKRIWMIQAHLGKAPEIDA"
     gene            complement(26319..27857)
                     /locus_tag="SCJ86_000685"
     CDS             complement(26319..27857)
                     /locus_tag="SCJ86_000685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318022.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gluconokinase"
                     /protein_id="HEG1168269.1"
                     /translation="MNVSIGLDIGTTQTKAVAFTDEAQEAASAYFRYPLIQETQGMAE
                     QDQELIFQGVCTVIKEVIKQLPSDTIIQFVSFSSAMHGLILLDEEKTPLSRMITWADN
                     RAADEAEKLKYTVLGKEFYRQTGTPIHPMTPLNKIKWLQNSQPELFKKTAYFVGIKDY
                     IFYRLFGELISDYGTASGTGYFDIHEFQWAEEVLDYLNIRIEQLPQVFPSTYQVTGLP
                     SKTAAELGLPNDISFVLGGADGPLSNLGLGAFGDTIAALTVGTSGALRFITTQPQFHP
                     EMETFCYVLDQKHWVVGGATSNGAGIFDWAGHTFLQEVVQSAKEKGENPYDALLSAIA
                     FVPAGANGLIFHPYLLGERAPLWDADAFGSFIGLQRQHTEKEMMKAILEGICLNLYRI
                     LKGVCQENQLTEIRATGGFAQSPIFRQMMADVLGYPLVFPTVTEASALGAVILGWRSI
                     GKIQSISEAQELIELKEQVGVQETDHKKYQKIYPLFVSTQKHLAQTYQQFAQLREELA
                     DSEK"
     gene            27937..28623
                     /locus_tag="SCJ86_000686"
     CDS             27937..28623
                     /locus_tag="SCJ86_000686"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016922532.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin peptidase"
                     /protein_id="HEG1168270.1"
                     /translation="MLLYFIIGCCIGSFLCLTAQRIPLGHSIIYPRSHCVKCCRSLSW
                     YELIPILSIIVQRFRCRYCRCRLPFYYLLAEALCGGLFAWFFTFSSARNFSTFIWMLS
                     ALLFSLMDLFYFMVHSHTLFCSWAILWIFWLQAGVFQWQSVLLTFIVGGVCLRYGQSY
                     LGAGDTLLMLSWSGGLSLEELLQILFLASLFGISVFSVYFICHKKKLEKLPFIPFLSS
                     ALLIVLHLRY"
     gene            complement(28824..29351)
                     /gene="msrA"
                     /locus_tag="SCJ86_000687"
     CDS             complement(28824..29351)
                     /gene="msrA"
                     /locus_tag="SCJ86_000687"
                     /EC_number="1.8.4.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339692.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide-methionine (S)-S-oxide reductase MsrA"
                     /protein_id="HEG1168271.1"
                     /translation="MTKKAVFAGGCFWCMVKPFEEQPGIISVTSGYTGGHVPNPTYEQ
                     VTTGTTGHTEAVEIEYDPEVISYDQLVEIYWQQTDPTDALGQFADRGDSYRPVIYYND
                     EEERNIAERSKQALQDSGRFDKPIVTKIEPRSEFYPAEDYHQDYYKKNKLHYNLYREG
                     SGRAGFLRRHWGVKK"
     gene            complement(29435..30112)
                     /gene="pgmB"
                     /locus_tag="SCJ86_000688"
     CDS             complement(29435..30112)
                     /gene="pgmB"
                     /locus_tag="SCJ86_000688"
                     /EC_number="5.4.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288121.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-phosphoglucomutase"
                     /protein_id="HEG1168272.1"
                     /translation="MKIKAALFDLDGVLVDTARYHYEAWLVLANQLSIPFTEKENEQL
                     KGISRTESLERLLSFGKMEQKFSEKEKSAFAEQKNNLYLQAIQKMDETSVLPGAIAVL
                     EYLKKTNIKIGLGSASKNARLILEKTNLTSYFDVLIDGTQVSKAKPDPEVFLKGAQQL
                     NVPPNACLVIEDSEAGCQAALAGNMHVLGIGENINLPSAEYVIPDLTVFDQVRSFWHL
                     SEAVSRI"
     gene            complement(30109..31239)
                     /locus_tag="SCJ86_000689"
     CDS             complement(30109..31239)
                     /locus_tag="SCJ86_000689"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302353.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerate kinase"
                     /protein_id="HEG1168273.1"
                     /translation="MRILIAPDSFKESLTALEAAQAIQSGFQEVFVQAEYDLLPIGDG
                     GEGTVSSLADSLRLKRQTAVVTNAFGKNKEIQYASDGQTAIFEMAEIVGLKDVPLNQQ
                     QPLRLSTRGVGELIRQLLQQGVRHIIIGVGGSSTNDGGIGMAEGLGYAFYDQDNQKVE
                     ALGENLGKIATVDKTNVPIELIKAKITVITDVTNLLCGKEGASFVFGPQKGLKENELA
                     VADQAMHDFYMRFAPQVIEASGSSAGGGMGAGLLAFASGKIVSGIDYVLDLLEFDKRV
                     QQADLVIVGEGRLDGQSLKGKAPVGIARRVPEGVPVIAICGSVGKGSEKAADVGISAV
                     FPILPAAVPLQEALQQATDNLHRTAKNIASLLQVVHFQGGNR"
     gene            complement(31239..33422)
                     /locus_tag="SCJ86_000690"
     CDS             complement(31239..33422)
                     /locus_tag="SCJ86_000690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320806.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 65 protein"
                     /protein_id="HEG1168274.1"
                     /translation="MKVEEKQFDLEYINKYASQMTIGNGFFGIRGSQEEDYRNQVRGM
                     FAAGVYNRPLGAESAELVNLPDVIRWEIKIDGEHFSLETMKVLAYERALDLEMGELKR
                     YALIETAQGKKVEVITCRVADQNNLHQAALKVVIKPLTEDCQVDVKTGIDGQQTNFGT
                     QQLVEKELRVFGDDLISASYQTTESKIDIGIAAKFNKAGVFQAKNRRITASFSEKIRK
                     NTSFTLEKIIVLSTSLQQTDPFKKSIAQAQKSVSYTEILEQSMSYWTDFWHQHRITIQ
                     GDSSFDQLAMDFAAYHLQIMTPRNDPSVSVGAKGLTGEGYKGHVFWDTEIFILPFFLY
                     QFPEVAKNLLLYRYDRLEGARQKARDYHYQGALFPWESAHTGQEETPKFAALNIKTGT
                     RQEVASAAAEHHISADLAYAVQEYVQATEDQSFLETKGKTLIHETAAFWLSRAVKVDD
                     HLEIHRVIGPDEYTEYIDNNAYTNYLAHYNVALAVKYQAGDPLFLKECQNFLDKLYLP
                     VPNKEQLIPQDDTYLSKPVIDLTTYKERPGSQSILLDYARSEVNELQVSKQADLVMLL
                     YLLPEYFSQPIVSKNLHYYENKTIHDSSLSKAIHAIEACRIQEMEWAYHLFQEACLID
                     LGPDPHSSDDGIHAASLGALWLAVIFGFSGITVTNGTLSISPRLPSAWEKISFPFIWK
                     NHRLLFVLTKKQIVIKKETKETLPIFVNGDQYQLEKELILERRGK"
     gene            complement(33419..34444)
                     /locus_tag="SCJ86_000691"
     CDS             complement(33419..34444)
                     /locus_tag="SCJ86_000691"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337111.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HEG1168275.1"
                     /translation="MKKVAIIGAGQVAEKVHAAYYQKRSDVLELAAVVDPVSQRAEDF
                     CRRTGFSHAYTSVTQMLNEISPDIVSVCTPNRFHFEHVLAALEANASVMCEKPPAMTA
                     DQARKMAETADKNGQILAYGFQHRFSDEATMIKNHLDVLGDIYYVEANALRRSGVPGW
                     GTFIDKELQGGGPLIDYGIHMLDTAMYITGFPKVKSVRAYSFQKIGTKKNSGKFGKWD
                     PSKFTVEDAIFGTLELENGKIIQLNTSFALNIREDKLNVAFCGDQAGANLYPGELFED
                     VDGKLVSLQKTTPVNDPHELSMAHFVNRALGDQTQMIADGWQGYVIQNLVENLYRSAE
                     TGEVITL"
     gene            complement(34489..35316)
                     /locus_tag="SCJ86_000692"
     CDS             complement(34489..35316)
                     /locus_tag="SCJ86_000692"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328799.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphate isomerase/epimerase"
                     /protein_id="HEG1168276.1"
                     /translation="MKFGTQDQAFFPRDILEKFRFIKDCGFEEYEIDGKLLIENIDTV
                     KQAIKETGIPVVTACNGYDGWIGDFIEERRLNGVRQIAEILRALSEVGGKGIVIPAAW
                     GMFTYRLPPMVSPRSKEGDYKAVSESLRYLDKVAEETGTVIYLEPLNRYQDHMINLLA
                     DARHYIDSNQLKHVQIIADFYHMNIEEDDISKALTTQKEYVGHIHLADNHRYQPGSGA
                     IDFTRHFQTLLQNEYEGYMVYECRLRGENEVKIYQESLQYLKNCLETARHLNDLEKE"
     gene            complement(35313..36380)
                     /locus_tag="SCJ86_000693"
     CDS             complement(35313..36380)
                     /locus_tag="SCJ86_000693"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-binding alcohol dehydrogenase"
                     /protein_id="HEG1168277.1"
                     /translation="MKKLVALEPRIAGFKDYEDREIEKNEVRIRVIFATPKPGTEIVD
                     FRGLSPFIDEEFSEEWRLFLPREEGSQRGVVFGEFQLGNMIVGTIEETGSLVTEYQKG
                     ELVCTYGPIRETLITNGVDNYKLRKLDRLDQWKNAVCYDPAQFALGGIREAHVRAGDA
                     VAIFGLGAIGQIAIQLAKKAGATTIVGIDPIAQRRELAEKHGADYTLDPMKADVGFRL
                     KELTDKRGVDSVIETSGSAAALQASLIGIGYGGIISYVAFAKPFPAGFNLGREAHFNN
                     AKIVFARASSEPNPDYPRWNRKRIEDTCWELLMNGYLDCEEIIDPVVPFAESAEAYMR
                     YVDQHPEESIKMGVQVRGGEQ"
     gene            complement(36406..37242)
                     /locus_tag="SCJ86_000694"
     CDS             complement(36406..37242)
                     /locus_tag="SCJ86_000694"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376388.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HEG1168278.1"
                     /translation="MKNKKSRKILFYATILLLLAVNLFPFLIMLMTSFKDSQEAIATT
                     QTIFPKEWTFEHYQDIFNAEIFPFIMYFKNSLVISLIASVTAVVIGISGAYALSKLRF
                     KARSKINASFYTVYMFSGILIVVPLFKIISGLGLYDTNTALIITMVVQTLPTAIFMLK
                     SYFDTIPTDIEEAAMIDGLSRIQIIIRIIIPLAISGIISVFVYCFMVSWNDYLFASIF
                     LSTSEKFTLPIGLNTLFSTPDYIWGRMMAASLVTALPVIIMYALSERFIKGDLVEGGV
                     KG"
     gene            complement(37239..38108)
                     /locus_tag="SCJ86_000695"
     CDS             complement(37239..38108)
                     /locus_tag="SCJ86_000695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HEG1168279.1"
                     /translation="MNRPRKHSDLKLAMGLLAPSMLLLAVLVLIPMLSNIQISFFDMP
                     LNPNSDAVFIGLSNYLEILSDSEFYRSLGLTVAYTFLVVAGSTALGLAVAILLNRKFR
                     FRKTVRSLILLSYVTPSISLIFAWKYMFNNSYGIVNDLLVDRLQLFNEAPLWFDQPVA
                     SFFMVVLFAIWRYYPYAFISFLAILQTVDKTLYEAAEMDGANAWQKFKIVTLPAIKPV
                     LATVIVLRSIWMFYMFTDVYLLTNKVNILGVYLYQTAFAFNDLGKAAAISILLFIIIA
                     AVVLLSRKKVDLK"
     gene            complement(38178..39461)
                     /locus_tag="SCJ86_000696"
     CDS             complement(38178..39461)
                     /locus_tag="SCJ86_000696"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter substrate-binding protein"
                     /protein_id="HEG1168280.1"
                     /translation="MKKIIVTTCLLATGFLLTACGESQSSNESQQTTIQFMHSSVEQE
                     RLSVINELVADFEKENPDIKIKQVPVEESAFNTKIVTLARSGKLPEVMEVSQDFAKVM
                     DKDELIDQTAVQNVIEEAGEDNYYDGAKNLVRSEDGKSYIAAPISGWVQGIWYDKEKL
                     SEAGFSEPENWDDILKIAQHFTDKENKQYGIAMPTAEGTMSEQAFSQFALSNGANVLD
                     DEGEVTIDTEKMREALSFYQNLSQYTMPGSNDVTEIKDAFMNGTAPMAIYSTYILPSV
                     YEAGNSENIGFAIPTQKDQAVYGTVSGLTISSGLDEKQKEAAEAFTAFLSQPKNMEKW
                     VLMSPGGAQPVNKQVVELDGYKENEVIKSFGELPSEIASAFDEVQVFGLVGEKNFTKM
                     GDITSSGVIGKAVNQVTVGNEDVDQALADAQKNTK"
     gene            complement(39475..41160)
                     /locus_tag="SCJ86_000697"
     CDS             complement(39475..41160)
                     /locus_tag="SCJ86_000697"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373849.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphorylase"
                     /protein_id="HEG1168281.1"
                     /translation="MYTKLLQAIYRDSDLSEKTASMIEQKVSVYQQKKGQAGKLHWDE
                     EDIFLITYGDQFFEEQQPTLRTFKKFYQKYLADTFRVVHFLPFFPYTSDDGFSVVNYQ
                     AINDRLGTWEDIEEMRSETRLMFDFVCNHMSAESTWFQEYLNLNDTYKDFFIDLPLDT
                     DTSSVTRPRTSPLLTTFIDKNGKEKHVWTTFSEDQVDLNFKNPEVLLKMIDILLFYLE
                     KGASFIRLDAVGFLWKELGTSCIHLPETHKIIQLFRAIADEAAPGTVLITETNVPHKD
                     NISYFGNGENEAQMVYQFSLPPLTLQAIRSGNTSKLKEWLKTIDFASKKTTFFNFLAS
                     HDGIGLNPVRGILPEKTILELVEQLKKEGARVNYKKNPDGTTSPYEINATYLDALSSP
                     TDTTEWRSKRFLAAHSFLVTLPGVPAIYIQSVLGSRNDYKGVEKTGENRKINRQKFLL
                     ADIEKELQTDPLRTAVYGELTQMLSIRKKEKAFHPNSLIYLLDSADSFISFIREAKEE
                     KVLVIHNFSNQSAGYLLPKGDYLDLFSGQIIHCSQERKLIFQPYEYMWLKIIK"
     gene            41435..42460
                     /locus_tag="SCJ86_000698"
     CDS             41435..42460
                     /locus_tag="SCJ86_000698"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324443.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HEG1168282.1"
                     /translation="MKPTIYDIAKKANVSKSTVSRVLNNQPNISDKAKASVLQAIKEL
                     NYEPSKIARGLSGNGFDAILALSHRTSHTTYGNPFFSDVIQSISAVSEQNNFDLILQT
                     AKTPEEELEKCAKKIQEKMIRGIIILSSTMDETLLFELDQYNIPIVVIGKVGNDYTNI
                     YSVDTDNFQDSYDLVETLIKNGHRHIGCLHAPTEVYVSNDRLNGYRQCLFDHHLDIRN
                     EWIIDGGFRLEDSMAAMNDLMKLRERPTAIFSTDALKSLSIYKVAEQSGKQIPDDLSI
                     AGFSDPLLSSLFSPQLTRIDIPTNQLGIKATELLFQLIHHQYPEEKRIILSTEITLTD
                     SIQQQEM"
     gene            complement(43055..46729)
                     /gene="rpoC"
                     /locus_tag="SCJ86_000699"
     CDS             complement(43055..46729)
                     /gene="rpoC"
                     /locus_tag="SCJ86_000699"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002367480.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit beta'"
                     /protein_id="HEG1168283.1"
                     /translation="MKWREPLLIDVNKFESMQIGLASPEKIRSWSYGEVKKPETINYR
                     TLKPEREGLFCERIFGPTKDWECACGKYKRIRYKGIVCDRCGVEVTRSKVRRERMGHI
                     ELAAPVSHIWYFKGIPSRMGLVLDMSPRALEEIIYFASYVVIEPGNTNLEKKQLLTER
                     EYREKREQYGQEFQAAMGAEAIKQLLDNVDLDAEAAELKEELKSAQGQKRTRAIRRLD
                     ILEAFRASGNQPSWMVMDVIPVIPPDLRPMVQLEGGRFATSDLNDLYRRVINRNNRLK
                     RLLDLNAPGIIVQNEKRMLQEAVDALIDNGRRGRPVTGPGNRPLKSLSHMLKGKQGRF
                     RQNLLGKRVDYSGRSVIVVGPFLKMYQCGLPKEMAIELFKPFVMRELVQRELASNIKN
                     AKRKIERQEDEVWDVLEDVIKEHPVLLNRAPTLHRLGIQAFEPVLVQGRAIRLHPLVC
                     EAYNADFDGDQMAVHVPLNEEAQAEARMLMLAAQNILNPKDGKPVVTPSQDMVLGNYY
                     LTMEEEGREGEGMVFRDQNEAVIAWRNGYVHLHSRIGVNPNSLGEKPFTEWQKERTMI
                     TTVGKIIFNEIMPPEFPYLNEPTDFNLTVQTPDKYFVEAGTDIPAHIKEQELVLPFKK
                     KNLGNIIAEVFKRFKVTETSKMLDRMKDLGYKHSTHAGITVGIADISVLNEKQEIIEN
                     AHKQVETITKQFRRGLITDDERYERVIAVWNAAKDSIQQKLMEGLEAKNPIFMMSDSG
                     ARGNISNFTQLAGMRGLMAAPNGRIMELPIISNFREGLSVLEMFISTHGARKGMTDTA
                     LKTADSGYLTRRLVDVAQDVIIREEDCGTDRGLEIQAIREGNEIIESLEDRLVGRYTR
                     KEVRHPETNELIIAGNQLISEDIAKQIVDAGVETVTIRSVFTCNTKHGVCKHCYGRNL
                     ATGSDVEVGEAVGTIAAQSIGEPGTQLTMRTFHTGGVAGDDITQGLPRVQEIFEARNP
                     KGQAVITEVTGDVIDISEDPATRTKEVTIKGKTDTRTYTVPYTARMKVAEGDMIHRGA
                     PLTEGSIDPKQLLQVRDVLSVENYLLREVQRVYRMQGVEIGDKHIEVMVRQMLRKVRV
                     MDPGDTDILPGTLLDIADFKDQNYNTLVSGGVPATSRPVLLGITKASLETNSFLSAAS
                     FQETTRVLTDAAIRGKKDPLLGLKENVIIGKIIPAGTGMQRYRDMEPKEVGVASENVY
                     SISDIEAQMAAEDAMNNQTK"
     gene            complement(46787..50404)
                     /gene="rpoB"
                     /locus_tag="SCJ86_000700"
     CDS             complement(46787..50404)
                     /gene="rpoB"
                     /locus_tag="SCJ86_000700"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334401.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit beta"
                     /protein_id="HEG1168284.1"
                     /translation="MAGHVVKYGKHRERRSFARISEVLELPNLIEIQTDSYQWFLDEG
                     LREMFEDILPIDDFNGNLSLEFVDYELKEPKYTVAEARAHDANYSAPLHVTLRLTNRE
                     TGEIKAQEVFFGDFPLMTEQGTFIINGAERVIVSQLVRSPGVYFHGKVDKNGKEGFGS
                     TVIPNRGAWLEMETDAKDISYVRIDRTRKIPLTVLVRALGFGSDDTIFEIFGDSETLR
                     NTVEKDLHKNASDSRTEEGLKDVYERLRPGEPKTADSSRNLLNARFFDPKRYDLANVG
                     RYKVNKKLDLKTRLLNLTLAETLVDPETGEIIVEKGTVLTHQVMETLAPFIDNGLNSV
                     TYYPSEDGVVTDPMTVQVIKVFSPRDPEREVNVIGNGYPEAAVKTVRPADIIASMSYF
                     LNLMEGIGNVDDIDHLGNRRIRSVGELLQNQFRIGLARMERVVRERMSIQDTETLTPQ
                     QLINIRPVVASIKEFFGSSQLSQFMDQTNPLGELTHKRRLSALGPGGLTRDRAGYEVR
                     DVHYSHYGRMCPIETPEGPNIGLINSLSSYAKVNKFGFIETPYRRVDRETGRVTDQID
                     YLTADIEDHYIVAQANSPLNEDGTFAQDVVMARAQSENLEVSIDKVDYMDVSPKQVVA
                     VATACIPFLENDDSNRALMGANMQRQAVPLINPQAPWVGTGMEYKSAHDSGAALLCKH
                     DGVVEYVDASEIRVRRDNGALDKYDVTKFRRSNSGTSYNQRPIVHLGEKVEKGDTLAD
                     GPSMEQGEMALGQNVLVGFMTWEGYNYEDAIIMSRRLVKDDVYTSIHIEEYESEARDT
                     KLGPEEITREIPNVGEDALKDLDEMGIIRIGAEVQDGDLLVGKVTPKGVTELSAEERL
                     LHAIFGEKAREVRDTSLRVPHGGGGIVHDVKIFTREAGDELSPGVNMLVRVYIVQKRK
                     IHEGDKMAGRHGNKGVVSRIMPEEDMPFLPDGTPIDIMLNPLGVPSRMNIGQVLELHL
                     GMAARQLGIHVATPVFDGASDEDVWETVREAGMASDAKTVLYDGRTGEPFDGRVSVGV
                     MYMIKLAHMVDDKLHARSIGPYSLVTQQPLGGKAQFGGQRFGEMEVWALEAYGAAYTL
                     QEILTYKSDDVVGRVKTYEAIVKGEPIPKPGVPESFRVLVKELQSLGLDMRVLDIEET
                     EIELRDMDDEDDDLITVDALTKFAEQQTAKELEKKAAEQVEDEKDDVIQNFETAEDNL
                     D"
     gene            51132..52100
                     /locus_tag="SCJ86_000701"
     CDS             51132..52100
                     /locus_tag="SCJ86_000701"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HEG1168285.1"
                     /translation="MKKNIIIGLGAALLLALGGCAQKDNSAEASDGQKTIGVLQLVQH
                     NSLDAAYKGFKEGLAEEGYKEGDNLTIDYQNAQNNQDNLKSMSEKLVKEDPDLLLGIA
                     TPAAQSLLNETTTIPMTVTAVTDLEQAHLVTSNEKPGGNVTGTTDMVPIEKQIGLLLN
                     IVPDAKTIGIMYNNGEANSKIQADLAEKELKKAGVKVKVLTANSTNDVQQVTKSLAKD
                     VDGIYIPTDNTFASAASVVGEVAKETKTPVVAGAVDQVKDGGLATVGIDYESLGKQTG
                     IMAAKILDGKNPSELPVETANDLELYVNKDMAKALGINPDSIKEAE"
     gene            52118..53023
                     /locus_tag="SCJ86_000702"
     CDS             52118..53023
                     /locus_tag="SCJ86_000702"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289390.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEG1168286.1"
                     /translation="MTNLLSLFLSSASQGVLWALLAIGVYVTFRILDIADLTAEGSFP
                     LGAGIAAVSITNGYHPLTACLLGFLGGAAAGLVSGLLHTKLKIPALLAGIITMTGLYS
                     VTSRVMGAPNISLLGQPTIFHWVESFGITKDNAVLIVGLLFALIVVCLLVLFLRTETG
                     LAIRATGDNLAMSEANGINTDNMKIIGYMISNGCIALSGSLLAQNNGFADLNSGIGTI
                     VIGLASIIIAEVLLRNQPLFLRLLTVVLGAVIYRFILALVFELNVEPSDSKLASAFVL
                     VICLSFPQIQQKFHLPQKKKGGSVK"
     gene            53020..53826
                     /locus_tag="SCJ86_000703"
     CDS             53020..53826
                     /locus_tag="SCJ86_000703"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348469.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HEG1168287.1"
                     /translation="MNDVLTLENLHHTFEKGTINENHVLKGINLSLDPGDFVTIIGGN
                     GAGKSTLLNSIAGTFSVDQGQILLNGKNITKKSVVERSKSISRVFQDPKLGTAVRLTV
                     EENLALAMKRGKKRGFFRGVKPQDRTFFKDQLVRLNLGLENRLSSEIGLLSGGQRQAI
                     TLLMATLQRPELILLDEHTAALDPATSKTVMELTEKLITEQNLTAFMVTHDMEDAIRY
                     GSRLIMLHQGRIVVDIQQEEKQQLTVPQLLALFKQNSGTALKDDQVLLSS"
     gene            53962..54951
                     /locus_tag="SCJ86_000704"
     CDS             53962..54951
                     /locus_tag="SCJ86_000704"
                     /EC_number="6.3.4.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318034.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biotin--[acetyl-CoA-carboxylase] ligase"
                     /protein_id="HEG1168288.1"
                     /translation="MGKELDAMTTKDKVLQLLKKTPDFLSGEKLAQQLEVSRTSIWKA
                     IKELEKAGYRFEHGPTGYRYLPSDVLDAKEIQQELRSFIPDLKVQTMLTSESTMKDAK
                     LAAAQGDSVSKLFVADTQEQARGRFGRPFFAEPGRGIYMSLLLHPDKRFDELPQYTII
                     AASACVKAIESVTGRSASIKWVNDIYMDGKKVCGILSEAVSDMESGRISHIIIGIGLN
                     FSVPQNHFPADLQEKAISVFPDGQASVTRNQLISKIWQELFTYLSHPEKALDFYKEKS
                     FVLGKTVSFNQKGTTYVGLAKEIGDHGELIVQTNEQKMSLSSGEISLTSIRTQ"
     gene            complement(55268..55792)
                     /locus_tag="SCJ86_000705"
     CDS             complement(55268..55792)
                     /locus_tag="SCJ86_000705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289384.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF664 domain-containing protein"
                     /protein_id="HEG1168289.1"
                     /translation="MDYLDMLCESVDRAYDRFERLFEGVSIEEANRFPVEDRAPQIKS
                     MTWLAWHTARELDFQIADLAGTSPIWHSQDWESRFPMTIADNEQDWKHSLEQAKQIQA
                     NQIEDLLGYLRGAADFAISYLRRLEPSELDEVIDENWTPPVTRGMRLVSIIDDAAMHS
                     GQVIYARRLLGLRD"
     gene            56079..>57190
                     /gene="scm"
                     /locus_tag="SCJ86_000706"
     CDS             56079..>57190
                     /gene="scm"
                     /locus_tag="SCJ86_000706"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="collagen-binding MSCRAMM adhesin Scm"
                     /protein_id="HEG1168290.1"
                     /translation="MKKRASLLVLVALLFNIFSYGVAVSAETYIPKDPGNIYNDLTGN
                     YGLLGIASQFHVFAKNKTTVNAHTDGNIATHELDAKNNFGTELHEGLLNQEINYIQKT
                     DSFANSSGIPTGSTRMNKFVVGSNVDVALQDGHPTVNGSRLDHLTPDDFYKDRAGHEY
                     IDFEQEFAKLNVAANDLATITPAKTYTAADFPDMNNRTIDLTDLSDTGFLLVNIDAEV
                     LTMNTPLQIINPNDQVVVFNVINSSSALNVQSPIKYNDRSNHETEDFSDANISWNFGN
                     EMTDLTISAPFQGTILAPNATIRITQNQDGTIIGTNVILDAATNRWDPNEIFISDNGT
                     DTTDTSDSTDTSTTSDSSDSSTSSDSTDTTSTTSDST"
BASE COUNT        17331 a        11543 c         9705 g        18611 t
ORIGIN      
        1 tctcattaaa atcaccagta aagaaatgga aatctttagt ttttttaata ttatcagtta
       61 gtaacttttt ataatcataa aaaaaataag tagttctatc tgagcgatac tcttttataa
      121 tactgaattc ggaattaaaa accacgatag gtaactttaa acaaacatga agagacttta
      181 ataagacttc tttatctgaa aacacttttg tttctctctt tcctttctac atcattaaat
      241 agtattttat acgttatcta aaatactatt cctctgtcaa aataagctaa cttacttcat
      301 tataaaatat ccacttatcg ctttcatgta tattccaccg taaaaagtac attttatcac
      361 cgagaactca tcatttttca tcatgatgaa tactatttac atatatctag ttcaaagaca
      421 ataattatta tgatccgtta ttatctaagc actatacaga taataggaat ttttcgtttc
      481 taaatattcc aatttgggaa tccaataaaa attttcgttc aaatacgtgt ctattcgaac
      541 tcttttctag ttgttaatta tgaaggggga agaaataaaa aatacaatct atacacgaat
      601 aagaatatta tccaaaagga ggttttagaa aagatttatt atacgtaaaa agctcaagac
      661 attttcttag ccataaggac atgtttgata tttaagaaga tatatgaatt ttcttttcta
      721 agtataaaga taactatcca ttatcaacta aaaaaacatt tttgaaaata aacatcagca
      781 gtcctatcaa tgcatgtgag cttatagaag ttattcgcag cgcagcataa ttgcattagc
      841 ttcttttcgt gttttttgcc tcttttccct ttaaatctta atggaggagg tgtgcaatat
      901 gtcagataac gacgaacgcg taaatgccca gttgattcga ttctttgaaa aggtcatttt
      961 gaacaccgct aatacttatt ataaaaaaca agaagaaatt caaaaacatg aacaacttga
     1021 tcaactccca ccatatttgt ttactaaaga tactataaaa tttaaacaac cgattaatgt
     1081 tttaaatgtg actctccttg ttgaagatat aaaattagca aaaatccttc ctttgttgaa
     1141 agaacctgaa caaatttttc ttatcgaaaa atttatcttt gataaaactg ataaagaaat
     1201 tggaaaggtt ctggggatta caagacaggg tgcgacaaat ttaaaacatc gactttacaa
     1261 aaaaattcaa aaaatgctgg tttaatatct gcataataac taattttgta tgcttctttg
     1321 ctcgttctcc tccttcccaa agaaatagat tataagtcca tgggaagaag gtagatttat
     1381 gaattcttta aaagaagatt tcattttagc aaaggctgga aatgaagaag ctgttgaggc
     1441 aattctcaaa agatttagct ctttgattca taagcaatcc tggagaactg gaaaatatga
     1501 tcaagattgt tatcaagaat gtatgcttgc aatatatcta gctatctcga aattcgaaat
     1561 taaagaataa ctcaactaag ggagtctgtg acataagtaa aaagatagtt tcaaatttcg
     1621 aacaataaat ttgaaactat cttctttata attataaagt atcgatttta cagcgttcta
     1681 taatgattca tagaatctaa aacgaatgat tttaattatc aacgtttttt aattcgtttt
     1741 tagcttaaaa ataacttttg tcacagtctc actcctaaaa ttactttatt tacgtatgaa
     1801 gtgggcagga ggtcgaatcc agaactgggc tatggtactt caacgaggac ttctcctcta
     1861 gaattgaaaa gcatgcgatt taccttccgt aacgacggta gatttattca actatgtgtc
     1921 caatcaaaat ttgtctgatt tcaaaaatag tcaagagaat tttggttgtt aaaaactctt
     1981 atctatcaac ttccacagtt tagttgacaa accctgatac taagttgggc tatgatacac
     2041 ttttcttgac tatcagttac gttaccggaa ggaatgaact ctattgagaa aaacatatga
     2101 tcgtaaattt aaactaaaga tttcacagga tatccttgag aaaaaagtaa ctaccaagaa
     2161 aattgctgaa gaatacaata tctctcgtcc aacaatttcc cgatgggttt cagaatatcg
     2221 tcgatacggg aaaaatgctt ttgctggaca aggaaaaaga ttacctgata aagcagattt
     2281 ctacattctt gaacaggaaa ataaacgact aaaagaggaa gacgagtgtt ctacgaacac
     2341 aaagatcgat acggatctcc agggatcact caaaaacttt acgatgaagg aatcgaaatc
     2401 aataaacgtg ttgtagctgt tcttatgcgt gaaatgaatt tatgcacaag aggatttcat
     2461 cgacgcaaat cttctcatgg tagagaaaaa acaatcaaag aaatggtaaa aaaatgttga
     2521 atcgataatt tgaacagaac caaattgatg ctgtttgggt gatggatatt acttacattc
     2581 catgtaatga cggtcgcctc tatttgtcca cctatattga cttagcaact cgtattctac
     2641 gttactatat ggtagattca cacatgaaaa aagagattgt cgtccgtcca ttacaaattt
     2701 attaaggaga attgcctacg gtaattcatt ccgatagagg tagccagcat cggtctttta
     2761 tttatcaaga gctgttgaac gaccaccaca tcacacattc agtgagccaa actgggactc
     2821 ctgttgacaa tgcagtaatt gaagcgtttc atcgatcaat caaatgagaa ttaatcgact
     2881 ccaacaaata taaaagtaaa atagagatga aggtgttaat ccaagattat ttagaagact
     2941 actacccaaa caaaagaatc catacaaaat ttatgatgac tccttgacaa tttgaagcga
     3001 agaaaataag ggatctataa tatctagtgt tggtgacgcc ttctgttgcc aatacttttt
     3061 cttttttcta ataaacgcaa acagacacct taaaatcctt tagaataaag tcgacgaaaa
     3121 ccattcaaag gagtgtttca agatgtccta ttcagagttt accaaagagt tactagatat
     3181 tctagaccta aatcttatct ttcatgaaga tgcctttcgc aaagagcgaa tcaatgacga
     3241 aacatgtttt gtttttgatg gcacattgac ctatcaaccg gaggaatgct ttcatcgcca
     3301 ctatcaaaat gaacaaacga tcattaaata gggctggaaa aaagtatcca tcttattgaa
     3361 tgatgtcagt aactacaaga ccattctgcg gattaacaaa cagcgcttca aatgtaaaca
     3421 ctgtggaaag acttttttag cagaagattc agttagtgat cgtcgctgct ccattgctcg
     3481 cagagtcaaa caagccattc ttgagctatt aagcgaacct ctttctatgt ctttgatcgc
     3541 acgtatgaag cacatttctc cgaccactgt cattcgaata cttcgcagcc ttcggcctaa
     3601 aaccatttct ttgagccagc tgttaccaga agtggtgtgt ttcgatgaat tcaaatcagt
     3661 gaaaaatgtc tcgggagcca tgagctttgt catgatggac ggcaagaccc atcaactgat
     3721 tgatattgtg gaaaatcggc aactcaatcc tttacgcgac tatttcttgc gctaccctat
     3781 aaaagtccgt gaacaagtgc gtctagtggt ttcagatttc tatacaccct atcgcacatt
     3841 ggtgaaagag tgttttccga atgcccgaat cgtcgccgat cgctttcata ttagccaaca
     3901 cattggtcgg gcttttacca accaccgaat tcaagtaatg aagaccttta aaaaagggga
     3961 tcgtcggtcg aagcacttaa agaaatactg gaaactactt caaaagaatg cgtgggaatt
     4021 gaaaggtcag catcgctact ggcgtccgtc atttcgagat catctgacag aagcagagat
     4081 agtcgatcgg ctattatctt atgatgattc cttaaagcga ggctacgaag tctaccagga
     4141 ttttctttcc gttatcagaa gacaagatgt tcctgaattc gtcgcgctcc taaaagaaga
     4201 ctacaaggag ttgccagagc actaccaacc agtgttcacg accttcaaga aataccaaac
     4261 agaaatcaaa cgagcgttgc gcgttcccta ttccaacggc cctatagaat gtttgaataa
     4321 ccatatcaaa gtactaaaac gaattgccta tggctataga aactttcaaa actaccgaga
     4381 acgaatcttt ttatatcgag gaaaatattt taagaaaact aagaatacta cccaactgac
     4441 taaagccaga acaacaacac ggctagacaa gatagctgtc tagccgtgtg agtatctcga
     4501 ttattctatt gaatttcttc accaacactc attgacgaag agccaaaata agtaatctaa
     4561 ccgactgtta actaaaccat tgcaggtccc gttaaagata cttgtgcctg ttgtcaccgc
     4621 ttctttttct tgtccacaag cgcttaacac acttaataaa gcccctgcta ttattaataa
     4681 taatatcttt ttcttattaa aaacctcctc atctatttcc agattagtaa atgtagtaat
     4741 ctgtgctttc ttttttctca atactaataa ctaacttatg gggtaaacaa atactcgttt
     4801 gaccgggttt tgagatccat ccagttctaa ccgcgatttg atcaggacta ttatcttctt
     4861 taactcgaat tcttccatct ttaatctcaa caatattata ttgtcccttc gcaggatgat
     4921 aattcacgag tttatgattg atcttatcca aatcgaagcg atccatttcc ttgccatcaa
     4981 ttcgaacgat ggcatatttg atagcatctt cagttgtttc attctccttt tctatagaga
     5041 atacaaataa gggaataaaa gaggtaataa tcaaaatgcc aataattatg acatcgccta
     5101 tcttgacacc agcttttctt aaaaatttca ttaacgatat ctggctaaac atattagact
     5161 ttttccatgg aaatggtgaa atttttcgga aattatctgc attaagagaa tcagttaaac
     5221 aaccctcaag ttgttcctta atacattgtt gatccatctt cataccaata aaaactagtt
     5281 tattaatgcg ttcaccaaac aacggatgcc aggcttcctt caattcggga tttttttgta
     5341 taatcatttg acactcaatt ttaggtaaac tagctaccca ataatttaat gattcaatac
     5401 ttttatagct acccgatgtt tcaaagatcg tcgaaaaatc actatgttgg gcaaaccaaa
     5461 gaaacccttt tgctcttgta atattgccag gaaaattttt atccgtaaga tgggcaaatt
     5521 tttgtgcgtc aaggagtaac cgatttcgat agataaaact tgagatacca tattcttctg
     5581 tttcaggaat atgattttga tagccaagct ctaactcttt gaaccagccc actgaatagg
     5641 aagtctcatc tatattaaaa agatttgtat ttagaatttt cgataagtct acttcacctt
     5701 taattgtcct aattattttg gcattggact gtaacttagc aattagaccg ttaattttgt
     5761 aaatcgtctt ggttgacact aagtcactat tatttaaaag taatatgtta caacactcga
     5821 attgatcaat taacaagcca cgaatatcct gttcttcttc aactacatta ccttcagctt
     5881 ttctttcctg aatcttttca cccgtttcga aattatccca aaaacgtttt acatccacta
     5941 ctataataat ggcaccaatc tctaatcgtt gggtcaaatc aatgtctaaa gggtttttag
     6001 ctaatataat tgcttgaata attggaattg gtttgctgat tcctgaagct tcaataacaa
     6061 tttgattcaa gtcagaaaat tgcactaatt gatctagttc aacaatcaaa tcttcgcgta
     6121 atgtacaaca aatgcatcca ttcggcatcg aaacaagttt ttctccagtt cttttaaaac
     6181 ctttcttgtc gactaatacc ccatcaacat ttacttcccc tatgtcattt acaatgacag
     6241 caatttcctt tccgtgatca ccgtacagaa tatgatttag aagtatcgtt tttcctgaac
     6301 caagatagcc agttaagatt gtaataggta tttttttcat agggaacctc tttctatacc
     6361 ttgaatttac actttaagtg caacacataa aaatgagtca taatcgatat ccaactagta
     6421 taagtttgtc gagaacactc ctagaaggaa aataattatg agccagtatc gtcatcttac
     6481 tatactcgaa cgagaaagaa tctttctgct tcacgaagaa ggattttcta ttagtataat
     6541 tggcaacgac attcagcata gcccgtttac tattttacat gaactgagaa gaaacaagaa
     6601 aaaaactatt caccttccaa agcggaaaaa aattatgctt atcgaagaaa aaaatgcgga
     6661 cgttatcaca ttctaaatga tccgtgagtt tggtcaattg ttcgtcattt atttgtcgat
     6721 tttcagtggt cgccagagga aatctcgaat cgtttgaagc atgaacatat ggacttttca
     6781 attagtttta tcacgatcta acgtgcaatt tgcaatgatc ttttcgatac agaacccctc
     6841 tcacacggca ataaaggcct gatccgttca ttgcaccatc gaggaaaaac aagacataca
     6901 aaaaatcatg tcgagtctcg tggaaagatt tgccttactc attcgatcca tggccgtcca
     6961 aaatccgcag atgatagaag tgattttggt cattggaaag ccgatattgt agctggcaaa
     7021 actggtggta cttgtttagt gactttgaca gacagaaaat catgcttcct tttagcagga
     7081 aagaaggtcc ccagaaagaa agccgcttat gtgagcgaga aaatgattga acttttaagc
     7141 agtattccaa atgaggcggt caaaacgatc actccagata gaggaaaaga attctccaat
     7201 catttcgcag ttacagaagc attgaataat atgccctttt attttccaga tcctcatgcc
     7261 cttggcaaca atgaacaaat taaaatacga atagactcat tcgagaatac ttgccaaaat
     7321 cgaaagagat agctacggta gataactcaa cgataaatca gatgattgaa aaactgaata
     7381 acagacctag aaagtgttta gattggagaa caccttatga agtcttcttt gataaagtgt
     7441 tgcgcttgat ttgacaattc aagacataat aaaaagcaga tcaaatccaa ataagggaaa
     7501 aatgatctac tctggtatta tcaattaatt ggccaagcta ttatgccgtt ttgctaaacg
     7561 tctacttctt ccagtattga ccttgataaa acaacaatca ggatcccgtt ttaatactct
     7621 atgaactgtg tcaatactaa catgtaaagt tgcagcaata tcagctaatg tactcccatt
     7681 taatctcatt tgctttgcaa atcgataacg ccgataagca tcaatattct tggaatccat
     7741 agacaataat acttttctta ccatgtattt cctccaaaaa ttgaatttta atgagccatc
     7801 atttcttgag caatttcatg tttagacaat gaagctggat agaaagtagg ccaattatgc
     7861 aattcattat agagtttttc ttgactttct ccaccaaaga aaatatggaa atgctctgcc
     7921 ttacttggtg caattccatg atcactaaat tgaatatatt taaaacgacc agcacctgca
     7981 tcatcagttt caaaattaaa acgaacacca cggtttcctt tttcataatt caaaattttg
     8041 tatccaacat atttgtatgt gtattgatga tgttcaccgt tgactacaaa atcaatggtg
     8101 ttgtctttaa tgttgatagt atcaatatct gtcttatagc cagtagtata ataatctttg
     8161 tactcggcag ctgtcatgtc tttattgatt tttgctttgt aatcgaaaac ttcatccaaa
     8221 attccatctt ttaacaacgg ataaacagat tgccattcgc ctgtataatc actcaattcg
     8281 cgatccttca catcggcatc tttaaagtac ccattagcaa ctgtcttttc atccttcgct
     8341 tctttttctg gaactacctc ttcaccagca accatagttg ttttttctaa cgcttttaga
     8401 ttgtcttcca ttactgaaac ataatcttca ccattatcca tttgttcttt tgtaagactt
     8461 tcaagaggat tcagtacttc caattttatc ccagcttcgt tggccaaagt ttttgcaatt
     8521 ttatcattag cattcttttc aaagtaaata taattgatct tatttttttt aacatactct
     8581 tttaattcag ctaatcgacc agaagatggc tcttcttcag gacttagtcc agcaatagga
     8641 acttgtttca atccataatc caaagctaaa tatccaaagg ctgcgtgttg cgtcacaaaa
     8701 cttttttgtt tcgcttcttt aagactagtg gtatagtcag catccaaccg tttcagttta
     8761 gtcaaatact tttcagcatt tgtctcaaat acttttgctt ttttcggata caatttaact
     8821 aactgatcct tgatatttga aacttcctta attgccattt tgggtgaaac ccacgtatga
     8881 ggatctagtt catgatggtg atcttcttct ccgtgatcat gatcttcttc ttcactacct
     8941 ggcaataaaa tcatgtcttt tgttccttct actacattag gcccagcttt cttccaactt
     9001 tctttagctt ttggtaccca tgattccatg ttttcgttat gatatacaaa aacatcagca
     9061 tcatgagctt ttgccatatc cttagctgag ggctcatagt catgaggttc tgatcccgct
     9121 ggaaccatta aatccacgtt cgcttcatcg ccgacgatgt ttttggtgaa atcatacatt
     9181 ggatagaatg ttgttacaat ttctaatttt ccatctttat cattcgatac ttttgcatca
     9241 ttagtgctgg agcaagctcc caataatcct agagctgcta tcccaataag tcctacactg
     9301 attttcttca ttctttttcc ttccttaatt atgctaattt cttcatttta tttcgtttat
     9361 gttctttgat aatttttaat gccctttttc tagttttaat attcgggctt ttaagtcgtc
     9421 gatatgcact agatatatcc gttttctcca ttagaaatta cccccaatca ctacttatca
     9481 ttatttgctt tccgccagta atttatcaaa actctgtttg atcctttctt catccaacat
     9541 ttttccgatt agaataatag tggtttggtc tttttgtcga gtggtgtatt gaaaattaac
     9601 ctgttcgttt acctcttgca tggcaactat aaaatcctgc tcttgtaaag caatcaatcc
     9661 tttaaaacga tacaggctcc cttggttggt taaaatcagc cagtcaatcc attggagtaa
     9721 aaaatctttt tttatgtctt gatctgaagt taagagaatt gatttaaact catgtacttg
     9781 atggtgttga tgatagtatt tgccattata gtgattttct gatgaatcac tttcaaaaac
     9841 taagggatga ttaaattttt tcaaattata aaaatccttt ttttgaatcg tttcactatg
     9901 tgtgaacatt cgaatatcgg ataaagggtt aatacttttg atttctctct gtaatttttt
     9961 tagtgcagat aaattcaagt tttgttttct agacacaaaa atgcgatctg ccatgacaag
    10021 ttgcttaagg gcttgtggtt ctctttcaat aagttcaaaa ctgtcactat caataacagt
    10081 caataaacta tcgatatata ctcttcctct tagttgaggc gctgttaaaa gtgtttgaat
    10141 gattggttgg ggatctgcta tacctgtagt ttcaatgatg atttgtgaaa ttggttgttg
    10201 atgttcttct attgtttcca gtaaagaaat caatattgct aataaatcac tgcgaagact
    10261 gcaacacata catccattat ttagttgtaa cacccgttcc tcgctatgaa ctaataattt
    10321 gtgatcaatc ccaacttcac caaactcatt ttcaattata ataatttctt ccctggggta
    10381 gggtgagact tgcaaggctt gattgattag cgttgttttt ccagccccta aaaacccaga
    10441 aacgattgta actggtatgc ccattttgtc acctttttac cacttagcta tttcacttct
    10501 ttaaaaattg cccgtctgcg caattttggc gaatactttt ttaattctaa ccgttcaggg
    10561 ttattccgtt tatttttgct ggtgagatac agacgttctc cagtttcgac acattccaaa
    10621 ataatatttt gacgcataaa tcttcctcct tcaatactta tcgatcttca agatatgctt
    10681 gaatttgttg tgcatcttca aaagttaact cgccagattc caattgtgaa actaaatttt
    10741 ctttcatatc aacaggtatc tcccaataac catcgataca ttgaaataac tcctcgtcat
    10801 actcttcaaa aattggatag attcttaaaa aatcatcaaa tgataagcct tggtcctcat
    10861 agtaatcttt gactaatcca ttttcaattg cctgttgggt cggtaaaccc gtttcagtaa
    10921 taattcctct ctctaccatc gaacgataaa aagtgcattt atctagcgat tgctgggtcg
    10981 tttcaagata atactttaat ccttcttcaa ctaaaggtgg gtaaataatt tccattttga
    11041 ttccctccta aaagttatat attaaactat tttgaagctc ctttaattac tttctttcca
    11101 ttatagtacc cttttaaaga tacatgatgg ctacgtctat aatcaccatt cgcttcgtca
    11161 aatgaaattt ctggttttac tacagtttga tgagttcttc ttaaccgttt cttcgcctta
    11221 gatgtttttc ttgctggtac tgccatgata tttcctcctt ttaccaactt gcttttttta
    11281 ctccaggaat caacccttga tgggcaagtt ctctaaactt aatacgggac ataccgaact
    11341 ttcgcatata ccctcttgga cgaccatccg tctgatcacg aagcctcaga cgattcggat
    11401 ttgaatctct tggcaattta gcgagagcgg tgcgatctcc tgcggctttt aattcctgac
    11461 gtttatctgc atacttttca atcatcgcca tttgtttctt tgctttagca attttcgact
    11521 tttttgccat atacctttat actcctttta ttttgattta gacacctcaa atcgtaatcg
    11581 ttacgttttg acactgatac tttaccaaca agagttgctc ttgtcaacaa taaataacat
    11641 gaaataaaac tcatatgaat agataactat ttgaaaacga atttatcggt acatagagat
    11701 aatcaaatca tttaaaagaa tatgcagttt aggctctgaa aaataagaat tcacttttat
    11761 tagtccaatc aaacacaaat accgttttaa gtagcttttc ctctacttaa aacggcaata
    11821 tgaaagaaac tataactttt tattcaatct tttatgttta actggtttat cgccaatttt
    11881 ttaaaacttt gattattcct tatattataa gaatagacat taagtgaact aattcaatca
    11941 tactgattct ttcgtgtatt caaaattaga tgctgtttaa taaaattatt ctttttgaaa
    12001 aagaattata actatatccc aaaaattgta ggttaattta aagcctattt catcatcgtg
    12061 attgagcttc tttttcatag acgaatttct cagatagttt agttttcttt atgagatttt
    12121 tgtatatttc tgaccttcga tacaactcat tatgagtacc ttgatcaact agttttcctt
    12181 gatccatcac tacaatttta tttacctttt caatagactt caagcgatgc gatataatta
    12241 gtactgtctt atcttgtatt aaacgattta aactttcctg aatacgtttt tcattttccg
    12301 catctaaact ggcagtaatt tcatctaaaa ttaaaattgg cgcatttttc aaaaaagccc
    12361 gtgctatgga taggcgttgt cgctctcctc cagaaagtga acttccgttt tctccgacca
    12421 atgtatgata accgtcaggt agtttttcaa taaagtcttc gcagttagct aaacgagctg
    12481 cttttttcac ttcctcatct gacgcttgac tatttccaat acgtatattt tccaaaatac
    12541 ttgtattaaa caaagttacc tcttgaaaaa caatagcgat tttgctaaac aaagaagctg
    12601 gagatacacg tttaatatca tacccatcaa ttaaaataca gccttcatca taatcataca
    12661 gtctagacac taatttcaag atactagttt tcccggaacc actagcacct actaacgcag
    12721 tcacttctcc ttgtttagcc gtaaaagaga tatgatctaa aattggggta ttgttatcat
    12781 atgagaaaga gacatctcgc aactcaatgt caaaagattt cagaggacta tctgatccct
    12841 cttggatact ggtttccttc atagctctaa ttcgctgtat tttaggagct aaataaaata
    12901 tttcaagcac tgcttctttc atagaatcga aggaatcctt tatttttatt gcggctagta
    12961 agtaaccaac gacgtagagt atagtcactt cacccgttaa cagtaagtga acacctacta
    13021 gaatgactac tgctaaacta acatatgaga aaatagagga taaggccatg actgaaaaag
    13081 tacttaattc tacctttaaa tgaatccttt cactctcttc catttttttg aaaagtctgt
    13141 cttgaacttt tttagataga ttaaagctat taatctcttg ctgcaattct atagtttctt
    13201 gaaattcttc cgagttttct ctcaaagtat cgtaatattt agtattggct ttcgtttggg
    13261 atttctttga taacaagatt aagacaaaac taaataacgt tggcaaaata acagctaatc
    13321 ccattttgac attaccaacc aaaagcatca ctgaaataaa agggaaaaac agagccatac
    13381 cgccggcttt aggtattgca tgactcatcg catgctcaat accttcaacg tcagacatga
    13441 tagtttgaga taaatctgat aaattatgtt ttgaaaaata ggataatggt aaatttgaca
    13501 aatcgtctgc aatttgcact cttaaatgcg cactttcttt ataagtacta ttatataagt
    13561 tttcatactc cctatctaac aaccaataca tgaaaagcaa agtaacaaac gatacggcaa
    13621 aataaagcca acggggcttt gtattttcta aaactaattc gtctatcagc atcattataa
    13681 tgaatgctgg taccatatta atgcagtata caaaaaaact agaaatagaa gctttagtta
    13741 aagcttctgc gccctgatca cttaaagcaa atttgttttt ataaaaactt ttcattgtca
    13801 agcctccaat catttgcatt ctcataatta gtgaccaatt gtttatacac ttgatgttta
    13861 gccattaatt cattatgatt tccacgttct actattttcc cttctgacaa gacaagaatt
    13921 tcatctacag atttaatgct ggataatcga tgagctatca taataactgt tttttctttc
    13981 atcagatgtt gaaaagcctt ttgtagttca tactcattat ctggatcaat agatgcactg
    14041 gcttcatcca taataacaat ctttgcatct tttaaaattg ctctggcaat agctatacgt
    14101 tgtttttctc ctcctgacag ataaactcct ttactaccaa ttaaggtatg ctctctttcg
    14161 ggaaattttt ctaaaatcga ttcgcaaccg gctaatctta gcgcgtcaaa tactttactt
    14221 gctggcgctt gaggatttgc tatagaaacg ttatcgtaaa tactctgatt gaatagtttc
    14281 gtatcttgaa agacaaaagc aattgtgtta ataattgtgt cttttgaata gtgatttaaa
    14341 ggcttaccac caattttaat ctgtccctta tccacattgt agaaacctga aattagttta
    14401 gctatcgtac tctttccgct tcctgatttt cctactaatg cataaattct tttttgttct
    14461 aaaactaagt tgaaattttc taaaacacac tcttctttgt atgaaaagct gacattttca
    14521 aatgcaatgt cacaattttt aatctgtgtc tcgttcccat attttagttg gtcttgcttc
    14581 atttctaaat aaagggagtc tagtgtttcg actgcatatc ttgcttcaaa caagtgcata
    14641 cttacataca ttattctcat gaaagaaaca aaaataattc cacttaaaaa aaacaacatc
    14701 aacaagtcaa cagctaatat cttgggagtg gtaagagaag ttataaaaaa ggttatagga
    14761 actatcaaaa tgcaaactat gccaaaaaat agccattgaa aaagaacaaa aggtgttttc
    14821 ccactcaaag aatacttata tgcaaactta gcataatcgt taattgcttt attcaaggct
    14881 ttaaatgatg ttactttagc accaaatatc tttataactt gaattccgcg gatatattct
    14941 accgtttcag agcttaacac atctaacgat tcttgatatt tttgaataaa atttgtattc
    15001 tctgtcattt tctttaacaa gaatacacca attactaaca tggcgattag aaccaatcca
    15061 attttaaagt taatgaaaaa gcctaaggca actgataaaa caggtgttac tatggcttga
    15121 gcactatctg gaatcaagtg agcaattgcc atatgtgttt tagccgcgtt gtcatcaatt
    15181 gtctttcgaa tatatcccga agaatttaaa tcaaaaaaac gaaaacttgc ttctgttaaa
    15241 ccgctaattc ccttttttct caaattagtc tctaagcgaa acccaaggat atgagaaaat
    15301 aagccagaaa aaatataaaa taaagctcta aagttaacca agcaacagtt tgaaccgcat
    15361 aactcttagc taaatattca tctcctacaa taattagaga ctgaagaaat ttgtaaatac
    15421 tgtaatatcc atagactgtt aatacgacag aaaaatcaga aaataaagtg gctaaccatc
    15481 cgtagtattt cattttaggg acataactaa ataatttttt gtatactttc attttttcct
    15541 cccccgcatt caatttcttc tcttattcgt ttacaactgt taatgctttc cgtaatagcg
    15601 taaattagag atgtacaaac agataaaagg taaactacgt tctatttttt gtattttttt
    15661 aagacttttc ttccaatcaa tacaccaatc atagataaac ttgctacaac taacactgaa
    15721 atgattcccc atatacctac cgcatagtgg atcattgcat ccattgttgc agcagattgt
    15781 cctttatcta tccaatcttg acgaaaacga tctgtaaaga accatatagg aatccactgt
    15841 ccggcagcac tgcctaattg cattagagcc caagacgcaa cttgaggaat tgctcgatca
    15901 tatccaaata cagatagaat aatatcagct aagatcgcca tactaatcga actgataaac
    15961 caaggtaagt acgccccgcc cattaacgta aaaaataaca ataaaacaat tgtgtaaata
    16021 gtaaaaattc cttttctagg gtagttatag ctaatataaa caaatatgat tccagataat
    16081 aatccaaaaa tcccggggga tatgctgtga ccaaacgccc caccaatact actaaccatt
    16141 attgaaaaaa agtaaattac tagggaaata actgtagcta aaccaataaa aatataatct
    16201 tttgttttca tatatattct cctcaattct atttcgttat gtcattaaaa atctgttcta
    16261 tcaaagttga atcttctact ctaagtttta tatctttttt tattccacca ttttttaaat
    16321 aaataattct attgcatgtt ttataaagaa attccggatc atgagtagct ataactactg
    16381 cacccttttc ttttaagtct ttaataaggt cagcaatcct ttccatatta atataatcca
    16441 acccacttgt aggttcatca aatagaaata aattagtttt tgacaatgaa gctatagaaa
    16501 ccaataatct ttgtttttct ccaccggata aatcaaacgg taattgatct ttcattttat
    16561 aaagttccat tttttttaaa attttttctt gctgatcttt actaactatt tcattcccca
    16621 gggctaactc gctcctcact gattctgtga aaaattggta atccatttct tgcattacta
    16681 aaaacggcga tgacaaattt tttttatttt cgattatctt tcctttggta gggcgaataa
    16741 tcttcatgag tattcttagt aaggttgttt tccccacacc gtttttccct accaaaccaa
    16801 taacatctcc aactttgaca tcaaaattta tgtcttttag aatattttca taagtaattt
    16861 cttcaacttt taatacatct gcacttacct tttcgatata gggaacttca atcgaactat
    16921 taaaaatgtc aaatttcctt aatgcgttat tgttttcagt ttttaacaac tgaatagttg
    16981 tagtttttac agttctattt ttaacaaata gaacttgatc aattaattct ttcaaatagt
    17041 aaaaacgatg ttcagctata acaatagtaa atcctttatt ttttaaatca caaagtaagt
    17101 cagcaagttt cttagtcgct ttgaagtcca aatttgaaga cggttcatcc aatataatta
    17161 atttagagtt tagcgataaa ctacatccaa tagaaatttt ctgccgttca ccacttgata
    17221 gcgaaaaaat gtttttatct aatatatttg aaagatctaa taattcagct aattcattga
    17281 ttcttttatc catatgctct tttgtaaatc caaagttttc catcgaaaat actaactcac
    17341 tcgttgtatt tgttgtaaag aattgtcctc taggatcttg acttacgtag ccaatatttt
    17401 ttgatatact ttcttgtgtt aaggattgta gcgagttatt ttcaaataaa atttccccaa
    17461 ccaattttcc gtcataaagt cttggaataa gtccattcat taactttaat agtgtggatt
    17521 tcccactccc agaagaacca gtaatcaatg taagactgcc tttttttatt gaaaaattaa
    17581 ggtcaacaag cgcttgttcc ttaacatcct tatatgaaaa agaaacattg ttaaattcaa
    17641 acaattcttt cacctcaaat caaaatactt aagaaaaaaa tagtaacgga aaacaggatg
    17701 atatagtcta ataagctcat gtttgtttta tagtaagaac ttcttttcac aggagattct
    17761 atcccctttg ttattgcatt agcggtcatt tcttcagcaa tcatagttgc acgtataaga
    17821 acgggaacta acagaaattc caaatattgc agaggctgaa aaaaagacaa gcgaatgttt
    17881 ctcattttta aagattcttt aatcattctt atttcagaac gataagtcgg aaagaatctg
    17941 atcactactg taatacttaa cattattttt ttcggtaaat gtattttttc taaagaggat
    18001 aataattcat tgctagtata gacactaacc aacaaccatg agatcattac gaccggcaac
    18061 attcgaaata caatatataa ggatgtcgca aaaaatatag taaaaaactc tatcttgata
    18121 gcatcaataa ttttcattac taaaaacaga actgaataaa tcaagatgaa ataaatacat
    18181 gtctttattt tacgaaacat caatactaaa actgtagcaa taatgaaaca ccccacttga
    18241 aatatatttc cctttcctaa aaaaataatg accggaaaaa aaatatttaa agtaatcaag
    18301 gttaacggat tgatttttga cactctttca cctccaaaaa ataagatcgc actgttatct
    18361 taagctgata aaaatgcacc gttattcaga taacagtgcg atcttatcaa aaagcataac
    18421 tgagaacaat tctcaatgca agcttttttt aattaactca gtaattcaat ccacccagga
    18481 acagaaaatt gatacaatct tctggttaat agcatagtct tttcttttga atagctatgc
    18541 ttcaatatat catagaatgt agcgtagaat gagtaaatgt agaagtggat ttcttcttca
    18601 ttaaagacta aattttgatt gatcattccg atagtcttta acttatctag acaacagact
    18661 gtctttttcg tcacaaatga tgtaattttt tcaacaatat cttgatattt ggttccagct
    18721 gattggaata aaatcaaatc gaatatacgt ttattctcaa aaatataatt aataaatgga
    18781 tctaattcat tgaagttctc tctccaactt ttctctatat ctatattttc taagtttaag
    18841 aggtatttgg taaactcttc attatacata ttaataagcg cattaatatg cggctgaata
    18901 atttcttcaa ataacgcttc cttagaagta aaatgtttat aaaaagcacc cgtagttacg
    18961 cctgcggtca ggcacagttg ccttatctta gtttgctgaa atccttcttt ttcaaaatgg
    19021 cttagtccac tttctattaa cttttggtga gttataatat aatctctggt ttctatgggt
    19081 acttcctcct aataagatga attaacttta ttatcttaca caggacttga agtttcaatc
    19141 gttaacgttt attactgaac tcattcctta gagctaaact aatgagaaag agcagaataa
    19201 ctgatacaaa aagcaatcct acaagaatta atagactcat ctaaatcatt caccttttct
    19261 aaaataatcc gagaaagatt taaaaaaatt atatccagct attaggataa gtcctatcat
    19321 aatcactcca atagccattt tcttatcctc cctaaaaata aagtctttta cttaaaacct
    19381 tttttgtaat cgtcaataaa ttttttccct ttatcagtta attgaaatcc tatatctaaa
    19441 tagtcctcat atctaagttt agccagcatc tcacttacag atgctggact aacacttaga
    19501 acttttgcta aatcattatt ttttaatttc ttttttcttt ttgataattc ctgaattgct
    19561 agtaaataaa ttagtatagt agtcgtttta atttgtacca tacctaccct ccaaattatt
    19621 tcgaaaggcc ttcagcaatt ttatccaggt tccatttcat catcgcatag taactatcac
    19681 catcttgtcc tttttctgca attgaatcag taaaaatcgt tgaatagata ggaatattgg
    19741 tatcttttga tactgttttc attggtctat catccacact gctttctacg aataaggagg
    19801 gaacttttgt tgtgcgtaat ttttcaacta agtgttttat ttgatctggt gttccttctt
    19861 cttcagtgtt gatttcccaa atgtaagcag aaggcacatt atacgctttc gagaaatatt
    19921 taaagcatcc ttcacttgta acaatcattt ttttatcatt cggaattgaa gcaaatttag
    19981 atttagcttc tttgtctaga gaatccaatt tttcaatata cttatctaga ttttctttat
    20041 agaatttttt attatcagga tctttttccg ctaactgttt ttcaatattt ttagcgtaaa
    20101 taataccgtt ttctaaattt aaccaagcat gaggatcttc cttccctttc tcactctgac
    20161 cctctaagta aataacatct atgccatcac ttgctgcaaa atagtctttg ttttcctctt
    20221 tgttcgcatt tttaactaat tttgtaaacc aagcatttcc tccagtttcc aagttaacac
    20281 cgttataaaa aatcaaatct gcttttgaag ttttttgaac atcttcaggc aaaggttcat
    20341 attcgtgggg atctttccca ataggtacga tactgtgtaa atcaatttta tcttttgcta
    20401 tattttcggt aatatctgct aaaatcgaat tagtaactac tacttttaat ttttcgttcg
    20461 aagccacttt atctttgtct ttactattta ttgaacatgc cccgaatata aaaagagcaa
    20521 tcaaaatccc cagacttccc agtactgctt tccattttct catcataacg atctctcctt
    20581 ctttcttaaa aatttttgtt ttggtgagat tagaaaacta attacaaata agaatgccgc
    20641 tgttaaaaca atacttgatc ctgctgctat gttaaaacta taaccaataa acaatccaag
    20701 caatgatgaa cttgctccga ctgttgcaga aataaatatc atagttttca agctatttgc
    20761 atataaatat gctgtagctg ctggtgtaat taacattgca acaattaata tagttcccac
    20821 actttgcata gctgttactg ctactaaagt taatagaacc atcaatagat aatgataaaa
    20881 actgacattc atccctattg cttttgccat taatggatca aaagaagtga ttaataattc
    20941 tttaaaaaat agaactacta taaccaacac taaaaccgaa acgccaattg taatccactt
    21001 atcaatatct tgtacagcta aaatatttcc aaataaaata tgaaataaat ccgttgagct
    21061 atttgcaact cctataagga taactcctag tgctaaaaaa gaactgaaag taataccaat
    21121 tgctgtatct ccttttatta cactattgct ttttataaaa gtaataatga tagaagctaa
    21181 tagaccaaat acaatcgcac caataaaaaa attaatacct aaaatataag aaattgctac
    21241 tcctggcaaa acagcatgag aaatagcatc acccataaga gacattcctc ttaaaataat
    21301 aaaacaacct actgttccag aaacaatccc tataactaca gatgtaatta gagcattctg
    21361 taaaaagtga aagtcataca gaccattaat aaaattttga agcatattat tccccctctc
    21421 caataaatat agtatctcca taggcttttt ttaaattttc ttcattaaat acagattcta
    21481 cacttccgtg agcaattaga tttcgattca aaagaataat attatcaaaa tacttcttta
    21541 ccttgcttaa atcatgatgc acaattagca ctgtttttcc ctgatgtttc aattttttta
    21601 acgtatccat aattattcgt tcactaacta agtctatccc tacaaatggt tcatctaaaa
    21661 atatgaaatc cgcattttga gcaagacatc ttgctaaaag tacacgttga aattgtcctc
    21721 cagataattc tcctatttga tgattagaat attttcccat atcgaccttt tccaaagcct
    21781 ttgaaactct ttgccattct gcatttttaa ttctttgaaa aactttcatt ccggcatatg
    21841 ttcccataga tacacattct tttactttta ttggaaaagt aaaatcaata tcagcttttt
    21901 gctctacata tgcaatcttc accagttttt tattgatatc ttcattatta atggagacct
    21961 ctccctgatg agggataata tttaacattg ctttaagcaa tgtggatttt ccagcaccat
    22021 ttggaccaat aatacctgtt aaagcacctt cttgtatttt taaagaaatc tcgttcaatg
    22081 ctaaaaaatc gttataacaa acattaagct ttttcacttc taacataact ttctccttct
    22141 aaatgataat gattatcacg atcatatggt atacaaaaat tatattttag tcaaccctaa
    22201 attttatagt ttagattttt ttctgttgct gaagattaga atgacaaaac tatttgaaac
    22261 tacgcgtata gtaagtagct ttcaaaatat ataaaaaagg agcgttctca aatgagaaca
    22321 tgctcctaaa actactttat tggcgtatgg aaaaattata cttgaagtgg atagcgggtt
    22381 gaataagttg ataagcatca aaacatttat aaaaattatt caagactatg tctgttccat
    22441 atacaaatat gaaaatcagt aaactccatt taactttttt cgtagtgtgt ctttttatac
    22501 atgtttatcg ttattaatag tgaataggaa aaaaactatt tttgatcatt gacaattaaa
    22561 tacaattttt gaataacgat ccctgctatt cgagctagaa actcaagtac gccatgacct
    22621 gattagcgac aaatacttag agatttcttt ttgttctggg aaaacaaaat gccatgacaa
    22681 tagcagcaaa taatgatact tccgtaaggc tcgccgtaaa ctggggagag gtgaaattcc
    22741 tatgtgctta cccaaagcaa ttcaaaaatt tcataactac tattttagga gatgatttta
    22801 tagaatccag agtctacctg aataaaaaac aaacctgtgc atttctagga atcgataata
    22861 atactctaga taagtggatt cgtatatata atttcccagt aataagagtt ggtggtgtag
    22921 ttcgctttga cgtagaagat attaaagact ttatgaatac atttaaaata acttgaaatt
    22981 ttcttctcgc cgcatatact aaaattagtg aaatatactg ccttactttt tttaaggagt
    23041 gaggattttg cctacaaagc tttcaaatgg gaaatataaa actaatctga gatatccaaa
    23101 aaggtttaaa gaaataacag gaattgcatc agaaaagtat caaaaaacat ttccaaatag
    23161 gcaattggcg attaaagccg aaaatgatat gaagaagaaa atagaaaaag ttttgagaga
    23221 ggagaatgca aactcattag aattaaaagg aaaaataacg tttaaaaaat tttacgaatc
    23281 taaatggctt cctagatatg aattaggtca aacaattaga tcaaacaggc ctcctagtga
    23341 tataaccatt agtaatacaa aagatatatt tcggttgcat attttaccta tgtttggtga
    23401 atacgcaatg aattatctaa atatcaatac tgagataata tcagatgaat taaccaaaaa
    23461 atcgaaagaa tatgcaaata taaaaatcat aaaaggatat gttcgttcca tgttcgatat
    23521 tgcagaaata ctaaattata tcgaatttaa tcgaactact aaaattattc aaagtatcac
    23581 tgcacctaaa aaaaatgctt tagaagaaaa aagaatacaa gaaggtaagc aagcactatc
    23641 atcaaaagaa cttactaatt ggatagaagc agtaaatgat gattttaata atcatttact
    23701 tacttttcat gattacacac tttttatgat taccctgtat cttggtgata gaaaaagtga
    23761 gacttacgct ttgcaatgga aatatattga ttttgaaaaa caaacggtcc gtcttaaaca
    23821 tactttagat aaatatcaaa gaaaaaagtt tacaaaaggt cgaaaagata ccgtcattca
    23881 agtaccagaa gttgtaatga cattgctaag tgaatggaaa tcggtacaag ctgatcaatt
    23941 gttgaaatta aaaattaagc aaacgttaga tcagtatctc tttacttata ctaaaccatc
    24001 tggagaagta aattgtcctg ttcatgccga ttacctaaac tatcgaatca attcaattaa
    24061 aagaaggcat cctgacttag ttcatttatc tccacacaaa ctccgtcata cttatgcaac
    24121 tatagctaga caaggtggag cagatatgaa tcaaatttca aatgctttaa cacactcaga
    24181 tatttctaca acaaagatat atgtgaacac accagatata gttgataagg ctgtttttga
    24241 agcttttcag agaggattga ataagtgtga ttgatatgtg ttcttaaatg tgttcttaaa
    24301 aacaaaagaa gcactttcca aataattgaa aagtgccaga aacgttgata tataaaggat
    24361 attaacgttt tgagaattgt gaagctttac gtgctttctt aagacctggt tttttacgtt
    24421 cgaccatacg tgcgtcacga gtaagtaagc cagcacgttt aagagctaaa cggaagtcag
    24481 gatcaacttc tagcaatgca cgagcgatac catgacggat agctcctgat tgtccagcgt
    24541 atcctccgcc gtttacgtta acaaatacgt catatgcgcc ttttgtttca gtaacagcga
    24601 aaggttgatt gattacttca cgcaagtcag catgtggaat gtattcttca acatcttttt
    24661 tatttacagt aattttgcca gttcctggta ctagacgtac gcgagcaact gcgtttttac
    24721 gacggcctgt gccgatatat tgaacttgtg ccaatgaaat tccctcctat tagattaagt
    24781 ttgtgatgtc taaaacttct ggttgttgtg ctgcatgtgg atgttctgct ccaccgtata
    24841 cgttcaattt tgtaaattgt ttgcggccta aagtgttttt aggaagcata ccttttacag
    24901 aagtttcgat caaacgacga gaatttttag cacgcaattc gccagcagtg attgatttca
    24961 atcctcctgg gtagttgctg tgacggtaat aaattttgtc agtcgctttt ttacctgtta
    25021 atttcacttt gtctgcattg atgacgatga cgaaatcgcc agtatccaaa tgaggtgtga
    25081 aagttggttt atttttacca cgtagtacag atgcaacaac tgttgaaaga cgtcccaatg
    25141 gaacatcagt tgcgtctact acataccatt tacgttctac ttcgcctggt ttggccatat
    25201 atgttgtacg cacgttattt tcctccaatt taagttctga tgtttgacat acacttcgag
    25261 tttccggggc tctttgtggg gcaaacaata ccatttaaca tattacccgc taatctagtc
    25321 aatgtcaacg tttttctgct tgattgaaaa atttatatga aactttactt cttttctttc
    25381 ttttatataa aagggtcgtt tcctgtcaaa atgaaaaaca aaaagaggct gtgacaaaag
    25441 tcttgtcaca accccttatt ttgaataaac ggtgttctcc tgccaactcc tcggtcacaa
    25501 gtcacaatat ccataaaaca taagaagctg ttttttaaaa ttgtttcttg tgactcggag
    25561 ctgaacggca ggcttacaac ctcttggtaa acggtgttca atcagctgac cctcggtatt
    25621 agctcaaaaa acggcaaaat atgaggaact attttgacgt ttgtttctct aatactcagg
    25681 tcaaaacgct gatttcacaa cctctttttc ggtcaagttc aacaacagtt tttacgcgtc
    25741 gatctctggc gctttgccca aatgagcttg gatcatccag atacgttttt ctgtggctgt
    25801 tttgaattcg ataaagatat cttgtgttgg atcgtcgcct tcttctccag aaacttcgat
    25861 tcctttttgg taaagatctg ctagataacg atagcctgca acaagtgttt ccaaacgttc
    25921 tggaatagta cgatcccagc ggccaatttc atcagggatt tttgtgttat ctgcaaattc
    25981 acgcaatgta gaataaggtg aaccgtctaa tgtgatcaag cgttcggata cttcatctaa
    26041 ctgagcgttc aaatcatcca tgaactcatc catcattggg tgcaatgtaa ggaaacctgg
    26101 tcctctcatg taccaatgtg tttggtgaac aacaacagac atttggctta gatctgcaac
    26161 taattgattt aatacttctt tggtttgttg gaatttcatc tttcttgcct cctaacattt
    26221 tcttacactt aaaataatag tagactttca atgaaaagta caacaaaatc atttacctga
    26281 gaacgatttt cagttcgctt ttttatccgt aaagccaatc atttttcaga atcagctaat
    26341 tcttctcgca gttgagcaaa ttgctgataa gtctgtgcta aatgcttttg tgtcgaaaca
    26401 aataatggat agattttctg atatttttta tgatcagttt cttgtactcc aacttgctct
    26461 ttcaattcta tcaattcctg tgcttcactg atactttgga tctttccgat gcttctccag
    26521 cctaaaatca cagctcctaa tgcggatgct tctgtgacag tagggaaaac aagcgggtaa
    26581 cccagtacat cagccatcat ctgacgaaaa atcggagatt gagcaaaccc gcctgtagca
    26641 cggatctctg tgagctgatt ttcctggcaa actcctttta aaatccgata aaggttgaga
    26701 cagatccctt ctagaatcgc cttcatcatt tctttttctg tatgttgccg ctgtaaaccg
    26761 ataaaactgc caaacgcatc tgcgtcccaa agcggtgctc gctcaccaag taaataggga
    26821 tgaaaaatca gaccattggc acccgctggc acaaaagcga tagccgaaag cagtgcatca
    26881 tatggatttt ctcctttttc tttagcagat tgaacgactt cctgtaaaaa tgtatgccct
    26941 gcccaatcaa agatgccagc accattgcta gtcgctcctc ccactaccca atgtttctga
    27001 tccagaacat aacaaaacgt ctccatttct ggatggaact gaggctgagt agtaataaaa
    27061 cgaagtgctc cgctcgttcc tacggtcaaa gcggcgatcg tatcaccaaa agcacctaac
    27121 cctagattag aaagcggccc gtctgcccct cccaaaacaa atgatatatc atttggcaaa
    27181 cctagttctg ctgctgtttt tgatggtaga cctgttactt gataggtgga aggaaagact
    27241 tgcggcaatt gctctattcg aatgttcaag tagtcaagca cttcttctgc ccattggaat
    27301 tcatggatat caaaatatcc agttcctgaa gctgttccgt agtcgctgat cagttcgcca
    27361 aacaatctat aaaagatata gtctttgata ccgacaaaat aagcagtttt tttgaataat
    27421 tcaggttggc tgttttgcag ccacttgatt ttgttcaatg gtgtcattgg atgaatcggc
    27481 gtcccagtct gccgataaaa ttctttgcca agcacggtat atttcagttt ttctgcttcg
    27541 tccgctgctc ggttatctgc ccaagtgatc attcgagata acggtgtttt ctcttcatcc
    27601 aacaatatca ggccatgcat cgcagaggag aaggagacaa attgaatgat tgtgtctgaa
    27661 ggcaactgtt taattacttc ttttataacg gtgcagacac cttgaaagat caattcctga
    27721 tcttgttctg ccatcccttg tgtttcttgg atcaaagggt agcgaaaata agcggatgct
    27781 gcttcttggg cttcatctgt gaaagcaact gctttggttt gtgtggttcc gatatctaat
    27841 ccgattgaaa cgttcatttt ttcacctcaa attttatgta tgtacaaaaa tttagtttaa
    27901 gcttgcgtat cttggttttg aaaaggggga gatggcgtgt tactttattt tatcattggt
    27961 tgctgtatcg gatcattttt atgtttaacc gcccagcgga ttccgttagg tcactctatt
    28021 atttatccta gatctcattg tgtcaaatgc tgtcgttcgc tttcttggta tgaattgatt
    28081 ccgattctct cgatcatcgt ccaacgattt cgctgccgct attgcaggtg tcggttgccc
    28141 ttctactatt tgttggcaga agccttatgt ggtggtcttt ttgcctggtt ctttactttt
    28201 tcttccgcaa gaaatttttc gacgttcata tggatgcttt cggctttgct attttcgttg
    28261 atggatttat tttacttcat ggtccattct cacacgctat tctgttcttg ggcaatttta
    28321 tggatttttt ggttgcaggc cggcgtcttc caatggcaaa gtgtgctgtt aacgtttatc
    28381 gtaggcggtg tgtgccttcg ctatggtcaa tcctatctcg gagctggcga tactctgttg
    28441 atgctttctt ggagtggtgg cttgtctcta gaagagttgc ttcagatctt gtttcttgct
    28501 agtctgtttg gaatatccgt tttttctgtt tactttatct gtcataaaaa aaagttggaa
    28561 aagctgcctt tcattccttt tttgagcagt gcgttgctga ttgtccttca tcttaggtat
    28621 tgaaaaaagg ttgtgacaaa cgacttgtca caacctctga tatatagtgt tctcttacca
    28681 aatccttgtg acttggagct aggcgacagg ttcacaacct ctccactaaa tggtgttcaa
    28741 tcagctgacc ctcagtatta gctcaaaatg ctggttttac aacctctgat atacggtgtt
    28801 ccataagtag ctttttcggc ttcttatttc ttgacgcccc aatgtcgacg aaggaaacct
    28861 gcacgtccag aaccttctcg ataaagattg taatggagct tgtttttctt gtaataatcc
    28921 tgatggtaat cttctgcagg ataaaattcg cttcttggtt cgatttttgt cacgattggc
    28981 ttatcgaatc gcccgctgtc ttgcaatgct tgttttgaac gctctgcaat atttcgttct
    29041 tcttcatcat tatagtagat cactggacga taagaatcac cgcgatcagc gaactgtcct
    29101 aatgcgtcag tcggatcggt ttgctgccaa tagatttcca ccaactgatc ataagaaata
    29161 acttctggat catattcgat ctctactgct tctgtatgcc ctgttgtgcc tgttgttact
    29221 tgttcataag ttggatttgg tacatgcccg ccagtataac cggatgtcac agaaatgatc
    29281 cctggctgct cttcaaatgg tttgaccata caccaaaagc atccgcctgc gaaaactgct
    29341 ttttttgtca tataaaatcc cccttttttc tcatcatacc ttgatttcca aaaggaggat
    29401 aacgttttgc ttgtgacaga aaaaacggaa actattatat acgtgatacg gcttctgata
    29461 aatgccaaaa cgaacgtact tgatcaaaaa cggttaaatc aggaatcaca tattcagcag
    29521 aaggaagatt tatgttttca cctattccca atacatgcat gttaccagcc aaagctgctt
    29581 gacaaccagc ttctgaatct tcaatgacca agcatgcatt tggcggtaca ttcaattgct
    29641 gtgctccttt gagaaaaact tctggatcag gttttgcttt tgatacttgc gtgccgtcaa
    29701 taaggacatc aaaataactt gtcaaattcg ttttttctaa aatgaggcgt gcatttttgc
    29761 ttgctgatcc caatccgatc ttgatgtttg tttttttcaa gtactcaaga acagctattg
    29821 caccaggcaa aacggatgtt tcatccattt tttgaatcgc ttgaagatat agattgtttt
    29881 tttgctcagc aaaggctgat ttctcttttt ctgagaattt ctgttccatt tttccaaatg
    29941 acagcaatcg ctctagtgac tcggttctgg atattccttt cagttgctcg ttttctttct
    30001 cagtgaatgg aatggacagc tggttagcta aaaccagcca cgcttcgtaa tgataacgag
    30061 ctgtgtctac aagaacgcca tctaaatcga atagtgctgc tttgattttc atcgattacc
    30121 tccttgaaaa tgaacgactt gaagtaaaga tgcgatgttt ttagctgtgc gatgaagatt
    30181 atcagtggct tgctgtaaag cttcttgtaa aggcacagct gcaggaagga tcggaaaaac
    30241 ggccgaaatg ccaacatctg ctgctttttc gcttccttta ccaacacttc cgcaaatagc
    30301 tatcacagga acgccctctg gtactcttct agcaatccca actggcgctt ttccttttag
    30361 actttgtcca tctagacgtc cttcgcctac gattacgagg tctgcttgct gtacccgttt
    30421 atcgaactcc agtaaatcca atacgtagtc gatgccagag actattttcc ctgaagcaaa
    30481 tgctaacagt cctgcaccca ttccaccacc agcactactg cccgatgcct cgattacttg
    30541 aggggcaaat ctcatataaa aatcatgcat ggcttggtca gccactgcta attcattttc
    30601 tttcaagcct ttttgtggtc cgaaaacaaa agaagcgcct tcttttccgc ataaaagatt
    30661 agtaacatca gtaatgacag tgattttggc tttaatcagc tcaataggca cgttcgtttt
    30721 atcaactgta gcaattttac ctagattttc tcccagtgct tctacttttt gattatcttg
    30781 atcgtaaaaa gcataaccta gaccttctgc catgccgatg ccgccatcgt tggtactact
    30841 tccgccaacg ccgatgatga tgtgtcgaac accttgttgc aacaattgtc gtatcagttc
    30901 tcctactcct cgcgtggata acctaagtgg ctgttgttgg tttagtggga catctttcaa
    30961 tccgacgatt tctgccattt caaaaatcgc agtttgaccg tctgaagcgt actggatttc
    31021 tttgtttttg ccaaatgcat tagtaacgac tgctgtttgc cgcttcaacc ttaaactgtc
    31081 tgctaatgat gaaaccgttc cttctcctcc atcgcctata ggtagcagat catattcagc
    31141 ctgaacaaaa acttcttgaa aacctgattg aatggcttgt gctgcctcta aggctgtcag
    31201 actttctttg aaagaatctg gcgcaatcag aatacgcatt attttcccct ccgttccaag
    31261 atcagttctt tttccagctg gtattggtct ccattgacaa aaataggtaa tgtttctttc
    31321 gtctcttttt ttattactat ttgttttttt gtcaaaacaa atagcaacct gtgatttttc
    31381 caaataaaag gaaaagaaat tttttcccat gctgaaggaa gtcttggaga aatagaaaga
    31441 gttccattcg ttactgtaat acctgaaaaa ccaaatataa ctgctagcca tagtgctcct
    31501 aatgaagcag catgaattcc gtcatctgat gaatgcggat ctggtcctaa atcaatcagg
    31561 cacgcttctt ggaataaatg atacgcccat tccatttctt ggatacggca agcctctatg
    31621 gcgtgaatcg ctttactcaa cgatgagtcg tggattgttt tgttttcata gtaatgcaaa
    31681 ttcttagaaa cgattggttg agaaaaatac tcaggtaata agtataacag catgacaaga
    31741 tctgcttgct tgctgacttg gagttcattt acttcgctac gggcataatc aagtaaaata
    31801 gactggctgc caggacgttc tttgtaagtg gtcaaatcaa taactggctt actgagatac
    31861 gtgtcatctt gcggaatcag ctgttctttg ttaggcactg gcaaatataa cttatctaaa
    31921 aaattttgac attctttcaa aaaaagcgga tcgccagctt gatatttaac agctaaggcc
    31981 acattataat gcgccagata gttcgtatat gcattattgt cgatatattc tgtgtattca
    32041 tctggaccaa tgactctgtg tatctctaaa tgatcatcga cttttacagc tctgctcagc
    32101 caaaaagctg ctgtttcatg aatgagcgtt tttccctttg tttctaaaaa cgattgatcc
    32161 tctgttgctt ggacatactc ttgaacggca taagcaaggt ctgcactgat atgatgttct
    32221 gcggcagctg aagcaacttc ttgtcttgtt ccggttttga tattcaatgc agcaaatttg
    32281 ggtgtctctt cctgaccagt gtgtgcgctt tcccatggga aaagtgcacc ttgatagtga
    32341 taatctcttg ctttttgccg tgcgccttct aatcgatcat aacggtacag cagcaaattc
    32401 tttgctactt caggaaactg atataaaaag aaaggcagga taaaaatttc cgtatcccag
    32461 aaaacatgcc ctttataacc ttcgccagtt agtcccttcg cccctacact aacacttgga
    32521 tcattgcggg gagtcataat ctgtaaatgg taagcagcga agtccatcgc taactgatcg
    32581 aaagaagaat cgccttggat cgttatccga tgctgatgcc aaaaatctgt ccaataagac
    32641 atcgactgct ctaaaatttc tgtataacta acgcttttct gggcttgggc aatacttttc
    32701 ttaaacgggt ctgtttgctg aagagaggta gagagcacga tgatcttctc cagcgtaaag
    32761 gacgtatttt ttctgatctt ttctgaaaag gaagcggtga tccggcgatt ttttgcttgg
    32821 aatacacctg ctttattgaa tttagctgca atcccaatat caattttact ttctgttgtc
    32881 tggtaggatg cactgatcaa atcgtcacca aatactcgca gttctttttc aaccaactgc
    32941 tgtgtaccaa aattggtttg ctgtccatcg atacccgttt tcacatctac ttggcagtct
    33001 tcggtcaacg gcttgatgac cactttcagg gctgcctgat ggagattatt ttgatcagca
    33061 acacgacaag taatgacttc cacttttttt ccttgagcag tttcaatgag cgcatagcgt
    33121 ttaagctctc ccatttctaa atctagtgcc cgctcgtatg caagaacttt catcgtctca
    33181 agagaaaaat gttcaccgtc gatttttatt tcccatctga tgacatctgg gagattcact
    33241 aactcggctg attctgctcc taaaggtcgg ttatatacac cagctgcaaa cattcccctt
    33301 acttgattcc gatagtcttc ctcctggctt cctctgatac caaaaaatcc atttccaatg
    33361 gtcatctgac tcgcatactt attgatatac tctaaatcaa actgtttttc ttctactttc
    33421 ataaggtaat aacttctcct gtttctgccg aacgatataa attttccacc agattctgga
    33481 tgacatatcc ttgccagcca tctgcaatca tctgagtctg gtcaccgagc gcccgattca
    33541 caaaatgagc catacttagt tcatgtgggt cattaactgg cgtggtcttt tgtaaggaaa
    33601 ccagctttcc atctacatct tcgaatagtt cgcctggata cagattagca cctgcctgat
    33661 cgccacaaaa agccacattc agtttgtctt ctctgatatt caaagcaaac gaagtattca
    33721 gttggatgat cttgccgttt tctaattcca gtgtgccaaa aattgcatct tctactgtga
    33781 atttgcttgg atcccatttc ccgaatttcc ctgaattttt tttcgtacca attttttgga
    33841 agctgtacgc ccgaacgctt tttactttag gaaatccagt aatatacatc gctgtatcaa
    33901 gcatgtgaat gccataatcg atcaaaggcc cgcctccttg aagttctttg tcgataaacg
    33961 ttccccagcc tggaacacca gaccgacgaa gcgcattcgc ttctacgtaa taaatatccc
    34021 cgagaacatc aagatgattc ttgatcattg ttgcttcgtc agaaaaacga tgctggaatc
    34081 cataagccaa aatctgcccg tttttatcgg ctgtctctgc catctttctt gcttgatcag
    34141 ctgtcatagc tggtggtttc tcgcacatca ctgaagcatt agcttcgagg gcagccaata
    34201 catgttcgaa gtgaaagcga ttgggtgtac agacactcac gatatcggga gaaatctcat
    34261 tcagcatttg tgtaactgat gtatacgcat gagaaaatcc tgtacgtcgg caaaagtctt
    34321 ctgctcgctg gctgactgga tcaaccacag cggccaattc taagacatct gatctttttt
    34381 gataataagc agcatgtact ttttctgcta cttgtccagc gcctatgatt gctactttct
    34441 tcatttattt ctctctcctt tgcttattta cttattgtta ttctttctct attctttctc
    34501 taaatcattt aaatgacgcg ctgtttccag acagtttttc aagtattgca atgattcctg
    34561 ataaattttc acttcatttt ctcctcgcaa acgacattca tataccatat aaccttcata
    34621 ctcattttga agtagcgttt gaaaatggcg agtaaaatca attgcgccag atcctggttg
    34681 gtagcgatga ttatctgcta aatggatatg acccacatac tctttttgcg tagtcaatgc
    34741 ttttgaaata tcatcttctt caatattcat atgataaaaa tcagcgatga tctgcacatg
    34801 tttcaattga ttagagtcaa tgtaatgacg cgcatccgct aacaaattga tcatatggtc
    34861 ttggtatcgg ttcaaaggct ccaaataaat aaccgtacct gtttcttcgg ctactttatc
    34921 taaataacga agtgattcgc tgactgcttt atagtctcct tctttgcttc ggggtgaaac
    34981 catcggcggt aatcgataag tgaacattcc ccaagctgct gggataacga ttcctttgcc
    35041 tccaacctcg cttagtgcac gcaaaatctc agcaatttga cgtacaccat taagacgccg
    35101 ttcttcgata aagtcaccga tccacccatc gtagccgtta caagctgtca ctactgggat
    35161 ccctgtttct ttgattgcct gtttgactgt gtcgatattt tctattaaca acttaccgtc
    35221 gatttcgtat tcttcaaatc cacagtcttt gataaatcta aatttttcta aaatgtctct
    35281 tgggaaaaat gcttgatctt gcgtaccgaa tttcattgtt ctcctcctcg gacttgaacg
    35341 cccattttga tactttcttc tgggtgctgg tctacataac gcatgtaagc ttctgcgctt
    35401 tctgcaaatg gcacaactgg atcaatgatt tcttcacaat ccaagtaacc gttcatcaat
    35461 aattcccaac atgtgtcctc gattcgttta cggttccatc ttgggtaatc tgggtttggt
    35521 tcactagatg ctcgagcaaa gacgattttt gcattattga aatgtgcttc gcgaccaaga
    35581 ttaaacccag ctggaaaagg tttagcaaag gcaacataag aaatgatccc gccatagccg
    35641 atccctatta aagaagcttg taatgcggca gcactgccac tcgtttcaat aactgaatct
    35701 actccacgtt tatctgtcaa ttcctttaga cgaaaaccga catctgcttt cataggatct
    35761 agcgtgtaat cggcaccgtg tttttctgct aattctctac gctgcgctat gggatcgatc
    35821 ccaacgatgg tagtagcacc tgctttttta gctaattgaa tcgcaatttg cccgatagcg
    35881 cctaaaccga agattgctac agcatcacct gctcgaacat gtgcttcccg gataccacct
    35941 aaagcgaatt gagcaggatc gtaacatact gcatttttcc attgatccaa ccgatcaagt
    36001 ttacgtagtt tataattatc aacaccgttt gtaatcagcg tttcacgaat gggtccataa
    36061 gtgcagacta actctccttt ttgatactct gtcaccaaac tgcccgtttc ttcgatcgta
    36121 ccgacaatca tatttcctaa ttgaaattcg ccaaatacca caccgcgttg gcttccttct
    36181 tctcttggca agaataggcg ccattcttct gaaaattctt catcgataaa tggacttaat
    36241 cctctaaagt cgacaatttc tgttcctggt tttggagtag cgaaaattac tcggatacgt
    36301 acttcgttct tttcgatctc acggtcttcg taatctttga atccggctat tcttggttct
    36361 aatgcaacta attttttcat cttttctttc ctctttcttt tctttttatc cttttacgcc
    36421 gccttcgact aaatcacctt taataaatcg ttcagataac gcatacataa tgatgactgg
    36481 taaagcggtc accaatgaag cggccatcat tcgtccccaa atgtagtctg gtgtactgaa
    36541 taatgtgttc aaaccgattg gtaaagtaaa tttttcggag gtcgacagga aaatcgaagc
    36601 aaataaataa tcattccacg aaaccataaa gcaataaacg aagacagaaa tgattccaga
    36661 aatagccaat ggtataatga tccgaataat gatctggatc cggctaagac catcgatcat
    36721 tgctgcttct tcaatatcag tcggaatcgt gtcaaaataa ctttttagca tgaatatcgc
    36781 agtaggaagt gtttgaacga ccatcgtgat aatcagtgct gtattggtat catacaaacc
    36841 tagaccggaa atgattttaa ataatggaac aacgatcaaa ataccggaaa acatataaac
    36901 ggtgtaaaag ctagcattga ttttcgagcg agctttgaag cgtagttttg acaaagcgta
    36961 tgccccgctg atcccaatca ctactgcagt tacggaggct atcaaggaga tcactaaact
    37021 atttttgaaa tacatgatga atggaaaaat ctcagcattg aaaatatcct gataatgttc
    37081 aaaagtccat tctttcggaa aaatagtttg tgtcgttgca attgcttctt gtgaatcctt
    37141 gaatgatgtc atcagcatga tcaaaaatgg aaacaaattg acagctaata gcaacaaaat
    37201 cgtcgcgtaa aataagattt tcctgctctt tttatttttc atttcaagtc caccttcttt
    37261 cgtgagagca agacgaccgc tgcaataatg atgaacaata gaatcgatat agctgctgct
    37321 tttccaagat cattgaacgc aaaggctgtc tgatacaaat aaacgcctaa gatattgact
    37381 ttattcgtca gtagatagac atctgtaaac atatagaaca tccagataga tcgtaaaaca
    37441 atcacagttg ctagtactgg tttgattgcc ggcagtgtca cgatcttgaa cttctgccaa
    37501 gcatttgcac cgtccatttc agctgcttca taaagcgttt tgtcgactgt ttgcaaaata
    37561 gccaaaaatg aaataaacgc atacggatag tatctccaga tagcaaacaa aacgaccata
    37621 aagaagctag caactggctg gtcgaaccac agcggtgctt cgttgaaaag ctgcaaccta
    37681 tctactaaca gatcattgac gattccataa ctattattga acatatattt ccaagcaaaa
    37741 atcaaagaaa tagatggcgt aacataactt aataaaatca gtgaacgcac cgttttccta
    37801 aatctgaact tgcggttaag caaaatagct acggctaatc ctaaagctgt actgcctgct
    37861 acaactaaga atgtgtaagc aactgttaaa cctaaagaac gataaaattc actatcactt
    37921 aaaatttcaa gataattgct taatccgata aaaacggcgt ctgagttagg attcagcggc
    37981 atatcgaaaa aactgatctg aatattggaa agcattggaa tcagcactaa aacagctaat
    38041 aaaagcatgc ttggtgctaa aagtcccatg gctaatttta agtcagaatg tttccttgga
    38101 cgattcattg aaacctcccc ctgataatct gccaatagct atctactttt ttcgaacacc
    38161 attggcagaa cttcttgtta tttcgtattt ttttgtgcgt cagctaaagc ttgatcgaca
    38221 tcttcgtttc cgacagttac ttggttcact gcttttccga tcacacctga gcttgtgata
    38281 tctcccattt tcgtgaaatt cttttccccg actagtccaa agacttgtac ttcatcaaaa
    38341 gcactagcaa tctcagatgg cagctcgcca aaagacttga tgacttcatt ttctttgtaa
    38401 ccatccagtt cgactacttg tttattcact ggttgagcac caccaggaga catcaagacc
    38461 cacttttcca tgtttttcgg ttgagataag aaagctgtga atgcttcagc agcctctttt
    38521 tgtttctcgt ctagaccaga agaaatcgtc aatccagata ctgtgccata gacagcttga
    38581 tctttttgtg ttgggatagc aaaaccgata ttttctgagt tccccgcttc ataaacagaa
    38641 ggcaaaatgt aagttgaata gattgccatt ggtgctgttc cattcatgaa agcatctttg
    38701 atttctgtca catcattaga gcctggcatc gtgtattggg ataagttttg ataaaacgac
    38761 aatgcttctc tcatcttttc cgtatcgatc gttacctcgc cttcatcatc caaaacattt
    38821 gctccatttg acaaagcaaa ttgagagaac gcttgctcac tcattgttcc ttcagcagta
    38881 ggcatagcaa ttccatattg tttattctct ttatctgtga aatgttgagc aatttttaaa
    38941 atatcatccc aattttctgg ttctgaaaaa ccagcttctg ataatttttc tttgtcatac
    39001 caaatacctt gaacccagcc gctaattggt gcagcaatat agctttttcc atcttctgaa
    39061 cgaacaaggt ttttggctcc gtcataataa ttatcttctc cagcttcctc gataacgttt
    39121 tggactgccg tctgatcaat cagttcatct ttatccatca ctttagcaaa atcctgactg
    39181 acttccatta cttctggtaa tttaccggaa cgagctaacg taacgatttt tgtattgaac
    39241 gctgattctt ctaccggcac ttgcttgatt ttgatgtctg gattttcttt ttcaaaatca
    39301 gcaaccagtt cattgatcac acttaatcgt tcctgttcga cagaagagtg catgaattga
    39361 atcgttgtct gttgtgattc gttagagctt tgactttcac cacaagcagt aagaagaaat
    39421 ccagtagcta gcagacaggt agtaacaata atttttttca tttttttcca cctcttattt
    39481 tattattttc agccacatat attcatacgg ctggaatatc aactttcgtt cttgactgca
    39541 atggataatt tgtccagaaa acaggtctag gtaatctcct tttggtaaaa gataacctgc
    39601 tgattgattg gaaaaattat ggataactaa gactttctct tccttcgctt cgcgaataaa
    39661 actgataaaa gaatctgctg agtccagtaa gtaaatcaaa ctattcggat ggaaggcttt
    39721 ttcttttttt cgaatagata gcatctgggt caattcacca tatactgctg ttcttaatgg
    39781 atctgtctgc aactctttct caatatctgc taacaaaaat ttttgccgat tgatttttcg
    39841 attctctcct gttttttcta cgcctttgta atcatttcga cttcccagca cactttgaat
    39901 ataaattgcc ggcacgcctg gtaacgttac caaaaatgaa tgagcagcta aaaagcgttt
    39961 gcttctccat tccgtcgtat ctgttggaga acttaacgca tctagatacg tagcgtttat
    40021 ctcatatgga ctggtcgttc catctgggtt tttcttgtag tttactcggg caccctcttt
    40081 cttcagctgt tccactaatt caaggatcgt tttttccggc agaatacctc gtaccggatt
    40141 caatccgata ccgtcatggg aggctaaaaa attaaaaaaa gttgtttttt tcgatgcgaa
    40201 atcgatggtt tttaaccatt ctttcaactt acttgtatta cccgaacgaa tcgcttgaag
    40261 ggtcaaaggt ggtagcgaaa actgatagac catctgtgct tcattctcgc cattcccaaa
    40321 ataactgata ttatctttgt gtggcacatt tgtctcagtg atcaataccg ttcctggagc
    40381 tgcttcatca gcgattgcac ggaataattg gatgatttta tgcgtttctg gcaggtgtat
    40441 acaagaagtt cctagttctt tccataaaaa tcccacagcg tccaagcgaa taaatgaagc
    40501 gcctttttct aagtaaaaca aaagaatatc gatcattttc aataaaactt ctggattttt
    40561 aaaattcaag tctacttgat cttcgctaaa agtcgtccat acgtgttttt ctttcccgtt
    40621 tttgtcaatg aacgttgtaa gtaacgggct ggtccttggt ctagtcacgc tgcttgtatc
    40681 tgtatccagt ggcaaatcaa taaaaaaatc tttgtacgta tcattcaagt ttaaatattc
    40741 ctgaaaccaa gtactttcgg cagacatatg attgcagaca aagtcgaaca ttaaccgtgt
    40801 ttcgcttctc atttcttcta tatcttccca tgtgcccaaa cggtcgttga ttgcctgata
    40861 atttacaacg gagaaaccat catcagatgt atatgggaaa aatggcaaga aatgtacaac
    40921 tctaaatgta tctgccaaat acttttgata aaatttctta aatgtacgca aagttggttg
    40981 ctgctcttca aaaaattgat ccccataagt aatcaagaaa atatcttctt cgtcccaatg
    41041 cagtttgcct gcctggcctt tcttctgctg ataaacactg actttttgtt caatcataga
    41101 ggcggttttc tcagacaaat ctgagtcgcg ataaatcgct tgtaaaagtt ttgtatacat
    41161 cggattcccc tttctttttt gggagcgttc ccaacgattt gatcatatta tggaaaacgc
    41221 ttactgtcaa tagcattttt ttaaataatt ttgatataca caaaaataca aataagaaag
    41281 cttatgtatc aataattaat agcttcttaa aaaaatatgg tctctcatga tcatgcaagc
    41341 tattcctaaa ttttcaaaat catagacaaa aaagaatgtc tattgtacta tggaattatt
    41401 ctaaaacgct cccaaaaagg aggtgcatat aaaattgaaa cctactattt atgacattgc
    41461 gaaaaaagct aatgtctcaa aatcaactgt ctccagagta ttaaataacc agccaaatat
    41521 ctctgataaa gcaaaagcaa gtgtacttca agcaatcaaa gaattgaatt atgaaccgag
    41581 caaaattgca agaggactgt caggaaatgg ttttgacgcg attctagcat tatctcatcg
    41641 tacttctcat acgacctatg gcaatccttt tttttcagat gttattcaat ccatttctgc
    41701 cgtttctgaa caaaataact tcgacttgat tctacaaacg gcaaaaacac cagaagaaga
    41761 gctagaaaaa tgcgcgaaaa agatccaaga aaaaatgatc cgaggaatta ttattttaag
    41821 ctctacgatg gatgaaacac ttctttttga attggatcaa tataatattc ctattgtggt
    41881 aatcggaaaa gtcggaaacg actacacgaa catctactca gtggacacgg ataatttcca
    41941 ggatagttac gatttggtgg aaacactgat caagaatgga caccgtcata tcggctgtct
    42001 ccatgctccg acagaagttt atgtatccaa tgatcgtctc aatggttacc gtcaatgctt
    42061 atttgatcac cacctagata tccgtaatga atggattata gacggcggct ttcgcttaga
    42121 agatagcatg gcagcaatga atgatttaat gaagctacga gaaagaccaa ctgcgatttt
    42181 ttctaccgat gcattaaaat cattgagtat ttacaaagtg gcagaacaat ctggaaagca
    42241 gatacctgat gatctttcga tcgcaggatt cagtgatccc ttattgtcct ccctattttc
    42301 gccacagttg actcggatag acattccgac aaatcagtta ggaatcaaag ccacagaact
    42361 tttattccaa ctaatccacc accaataccc tgaagaaaaa agaatcattc tttcaaccga
    42421 gattactctc acagattcga tccaacaaca agaaatgtag aacagaatta taaaagaaca
    42481 gaaacactga ggataagtca gttactctgt tcttttttca atcactcatc cctcgttacc
    42541 ccctcccggt attttagcga atttttctta ttttccatct gttttagata gctgtggata
    42601 actataatgt ttgtgttaaa ctattttttc cacagggaag aaaaaactat ttttaaccac
    42661 gttgtgtata agtttttata gttcgttttt ttaaacacat ttacccttat ttcaatccat
    42721 gttatttggg gatacttatc tctaaaacat aaatagaata acagacatgt ctactggttt
    42781 tgttttattc gaggaattat tcaataatag ctagcaaaat attaaaaata accgctctat
    42841 cttcccatca atttattttg cattcaaaaa atgatctatc tttacttttg ttgattgact
    42901 tctcttggaa gatgatcgtt ccgatacctt aattggatac gaaccgatgt tgttaagatc
    42961 ctttcccact tttcttctag ttagacaaat tattttagac aaaccaaaaa agaggttgtg
    43021 acaataattt gtcacaacct cttttttaaa atatctattt tgtttgattg ttcattgcgt
    43081 cttctgcagc catttgcgct tcaatatctg aaatactgta gacattttcg cttgctacac
    43141 cgacttcttt tggttccatg tcacgataac gttgcatacc agtaccagct gggatgatct
    43201 tacctataat aacattttct ttcaaaccaa gaagaggatc tttcttaccg cggatcgcag
    43261 catccgtcaa gacacgagtt gtttcttgga atgatgcagc agataagaaa ctatttgttt
    43321 ctaatgatgc ttttgtgata cctagtaaga caggtcgaga agttgcagga acgcctcctg
    43381 agactagcgt attgtagttt tgatctttaa agtctgcgat gtctaataat gtacctggta
    43441 agatatctgt atcacctgga tccattacac gaactttacg cagcatttga cgaaccatta
    43501 cttcgatatg cttgtcgccg atttctaccc cttgcatacg gtaaacacgt tgtacttcac
    43561 gaagcaagta gttttcaaca gataatacat cgcgaacttg caacaattgt tttggatcga
    43621 ttgatccttc tgtcaatggt gcaccacggt ggatcatatc gccttctgct actttcatac
    43681 gtgctgtata aggaacagta tacgtacgtg tatctgtttt tcctttgatt gttacttctt
    43741 tggtacgtgt tgcaggatct tctgagatat cgatgacatc ccctgttact tctgtgatca
    43801 ctgcttgccc tttcggatta cgtgcttcaa agatttcttg gacacgagga agaccttgag
    43861 tgatatcgtc tccggcaacc ccacctgtat ggaatgtacg catcgtcaac tgagtaccag
    43921 gttcaccgat tgattgagcg gcgatcgtac caactgcttc tccgacttca acgtcagagc
    43981 cagttgcaag gttgcgtccg taacaatgtt tgcagacacc atgttttgta ttacatgtga
    44041 agacagaacg gattgtcact gtttcgacac ctgcatcaac gatttgtttt gcgatgtctt
    44101 cggaaatcaa ttggtttcct gcaatgatca attcatttgt ttcaggatga cgtacttctt
    44161 tacgggtgta acgtcccact aaacgatctt ctaatgattc gatgatttca tttccctcgc
    44221 ggatggcttg gatttcaaga cctcggtcag taccgcaatc ttcttcacgg atgattacat
    44281 cttgggccac gtcaaccaga cgacgagtca agtaacctga atcggcagtc ttcaaggctg
    44341 tatcggtcat ccctttacga gccccgtgag ttgagataaa catttccaag acggatagtc
    44401 cttcacggaa gttcgaaatg attggcaatt ccatgattcg accattagga gcggccatca
    44461 aaccacgcat tccggcaagc tgtgtaaagt tggaaatgtt accacgagct ccagaatcag
    44521 acatcatgaa gattggattt ttcgcttcca aaccttccat cagtttttgt tggatgctgt
    44581 cttttgcagc attccaaaca gcaatgacac gttcataacg ttcgtcatct gtgatcagac
    44641 cacgacggaa ttgttttgta atagtttcaa cttgtttatg ggcattttca atgatttctt
    44701 gtttttcatt cagtacactg atgtcagcga tccctactgt gataccggca tgtgtcgaat
    44761 gtttgtaccc taagtctttc atacggtcaa gcatcttaga agtttctgtt actttgaatc
    44821 gtttgaagac ttctgcgatg atgtttccta agtttttctt cttgaacggt aatactaatt
    44881 cttgctcttt gatatgtgca ggaatatcag taccagcttc aacaaagtat ttgtccggtg
    44941 tttggacggt caagttgaag tctgttggtt cattcagata agggaattca ggaggcatga
    45001 tctcgttgaa gatgatctta ccaactgttg tgatcatcgt acgttctttt tgccattcag
    45061 taaatggttt ttcacctaat gagttagggt tcacaccgat acgtgaatgt aaatgaacat
    45121 atccgttgcg ccatgcgata acggcttcgt tttgatctct gaagaccatt ccttcgcctt
    45181 cgcgtccttc ttcttccatt gtcaagtagt agttacctag aaccatatct tgagaaggag
    45241 taacaactgg tttaccatct ttagggttca agatgttttg agcagctaac atcaacatac
    45301 gtgcttctgc ttgtgcttct tcattcaatg gaacgtggac agccatttgg tctccatcga
    45361 aatcggcatt ataagcttca catacaagtg gatgcaaacg gattgcgcgt ccttgtacta
    45421 aaactggttc gaatgcttgg ataccaagtc ggtgaagcgt aggtgcccgg ttcagcaata
    45481 ctgggtgttc cttgattacg tcttctaaaa catcccatac ttcgtcttct tgacgttcga
    45541 ttttacgttt cgcatttttg atgtttgaag ctagttcgcg ttgaacaagc tcacgcatca
    45601 cgaatggttt gaataattca atggccattt cttttggaag tccacattgg tacatcttca
    45661 agaaaggtcc aacaacgata acggaacgac ctgagtagtc gacacgttta cctagcaagt
    45721 tttgacggaa acgaccttgt ttccctttca acatatgaga aagagatttc aatgggcggt
    45781 tacctggtcc agtaactgga cgtccgcgac gaccgttatc gatcaatgca tctactgctt
    45841 cttgcagcat acgtttttca ttttgaacga tgattccagg tgcattcaag tctaacaaac
    45901 gtttcaaacg gttattacgg ttgattacac gacggtaaag atcattcaag tcacttgttg
    45961 caaaacgtcc accttctaat tgaaccattg ggcgtaaatc tggtggaata acagggataa
    46021 cgtccataac catccagctt ggttgatttc ctgaagcgcg gaatgcttct aaaatatcca
    46081 aacggcggat tgcacgtgta cgtttttgtc cttgtgcaga tttcaattct tctttcaatt
    46141 cggctgcttc tgcatctaga tcaacattat caagtaattg tttaatcgct tctgcaccca
    46201 tagcagcttg gaattcttgt ccatattgtt cacgtttttc gcgatattcg cgttctgtca
    46261 acaattgttt tttctctaaa ttcgtatttc ctggttcgat gactacatat gaagcaaaat
    46321 aaatgatttc ttccaaagcg cgtgggctca tgtctaatac aagacccata cgtgaaggaa
    46381 tccctttaaa gtaccagata tgtgaaacag gagctgctaa ttcaatatgt cccatgcgtt
    46441 cacggcgaac tttcgaacga gtaacttcca ctccgcatcg atcacaaacg attcctttat
    46501 aacggatacg tttgtatttt ccgcaggcac attcccagtc ttttgtagga ccaaaaatcc
    46561 gctcacagaa caacccttca cgttcaggtt ttaaggtacg atagttaatc gtctcaggtt
    46621 tttttacttc accgtaagac cagcttctga ttttttcggg agaagccaaa cctatttgca
    46681 tactttcgaa tttatttaca tcgatcaaaa ggggttccct ccatttcatt taatggcaaa
    46741 ccgcaagacg gctgatcttc gaccgccttg cagctcatag accggattag tctaaattat
    46801 cttctgcggt ttcaaagttt tggatgacat cgtctttttc atcttccact tgttcagcag
    46861 ctttcttttc taattctttg gcagtttgct gttcggcaaa ttttgtcaaa gcatcaacag
    46921 tgatcaaatc gtcgtcttca tcgtccatgt cgcgaagttc gatctctgtt tcttcgatat
    46981 ccaagacacg catgtccaga cctagtgatt gcaattcttt caccaatacg cggaatgatt
    47041 ccggtacacc tggttttgga attggttcac ccttgacgat tgcttcgtat gttttcacac
    47101 gtccgactac gtcatctgat ttgtatgtca agatttcttg taaagtataa gccgcaccgt
    47161 aagcttccag tgcccatact tccatttcac caaaacgttg tccaccaaat tgagctttac
    47221 ctcccaatgg ttgttgtgta accaatgagt aaggtccgat tgaacgtgca tgcaatttgt
    47281 catcgaccat gtgagcaagt ttgatcatgt acatcacacc gacagacaca cgtccatcaa
    47341 atggttcacc tgtacgtccg tcgtacagaa cagttttcgc atcgctggcc ataccggctt
    47401 cacgaactgt ttcccatacg tcttcatcac ttgcaccatc aaatactggc gttgctacgt
    47461 ggatacctaa ctgacgtgca gccataccca agtgtaattc caatacttgc ccgatgttca
    47521 tacgtgaagg cacccctaaa ggattcagca tgatatcgat tggtgttcca tcaggtagga
    47581 atggcatatc ttcttccggc ataatacggg atacaacccc tttatttccg tgacgaccgg
    47641 ccattttatc cccttcatgg attttacgtt tttgaacgat ataaacacga actaacatgt
    47701 ttacgcctgg tgacaattca tctccagctt cacgagtaaa gattttcaca tcgtgaacga
    47761 taccgccgcc gccatgtggt acacgtaatg atgtatcgcg gacttcacga gctttttcgc
    47821 caaagatcgc atgcaataga cgttcttctg cagataattc tgttacccct tttggcgtga
    47881 ctttccctac aagcaaatcg ccatcttgga cttctgcacc aatacggatg atacccattt
    47941 cgtctaaatc ttttaatgcg tcttccccga cattagggat ttcgcgtgtg atttcttcag
    48001 gtcctaattt tgtatcgcgg gcttctgatt catattcttc aatatggata gaagtgtata
    48061 cgtcatcttt taccaaacga cggctcatga tgatcgcatc ttcatagttg tacccttccc
    48121 aagtcatgaa accaactaaa acgttttggc ctagagccat ttcgccttgt tccatagatg
    48181 gtccgtctgc cagagtatcc cctttttcga ctttctcacc caaatgaacg attggacgtt
    48241 ggttgtagct tgttccggag tttgaacgac ggaattttgt tacatcgtat ttatctaatg
    48301 caccgttgtc gcggcgtacg cggatttcag aagcatcaac gtattcaacg acaccgtcat
    48361 gtttacataa caaagcagct cctgagtcat gcgctgattt gtattccata ccagtaccaa
    48421 cccatggtgc ttgaggattg atcaacggca ctgcttgacg ctgcatgttg gcacccatca
    48481 aggcacggtt ggagtcatcg ttttccaaga aaggaataca tgctgtcgca acggcaacta
    48541 cttgtttagg tgatacgtcc atgtagtcta ctttatcaat agatacttct aagttttcac
    48601 tttgagcacg agccataaca acgtcttgag caaatgttcc atcttcattc aatgggctgt
    48661 ttgcttgggc tacgatgtaa tggtcttcga tatcagctgt caagtagtcg atttggtcag
    48721 taacacgacc tgtttcacgg tctacacgac gataaggtgt ttcgataaat ccgaatttat
    48781 ttactttcgc ataactggac aaactattga tcaacccgat gtttgggcct tcaggagttt
    48841 cgatcggaca catacggcca tagtgggagt agtgaacgtc acgaacttca taaccggcgc
    48901 ggtcacgagt caatccgcca ggtcctaagg ctgaaagacg acgtttgtgt gtcaattcgc
    48961 caagagggtt tgtttgatcc atgaactgtg acaactgaga agaaccaaag aattctttga
    49021 tacttgccac aactggacgg atattgatca attgttgagg tgtcaatgtt tcagtatctt
    49081 ggatcgacat tctttcacga accacacgtt ccatacgtgc tagcccgata cggaattggt
    49141 tttgtaaaag ttcaccgact gaacggatac gacgatttcc taagtggtcg atatcatcga
    49201 cattgccgat accttccatc aagttcaaga aatagctcat tgaagcaatg atgtcggctg
    49261 gacgaaccgt ttttacagct gcttctggat agccgtttcc gatcacgttt acttcacgtt
    49321 cagggtctct tggtgaaaat actttgatga cttggacagt cattggatct gtgaccacac
    49381 cgtcttctga tggatagtaa gtcactgaat ttaggccatt atcaatgaaa ggagctagtg
    49441 tttccatgac ttggtgagtc agaactgttc ctttttcaac gatgatctcg cctgtttctg
    49501 gatcaactaa tgtttcagct aatgtcagat tcaatagacg tgttttcaaa tctaattttt
    49561 tgtttacttt gtaacgacca acgtttgcta agtcgtaacg ttttggatcg aagaaacgag
    49621 cattcagcaa gttacgtgag ctgtctgctg tttttggttc gcctggacgc aaacgttcgt
    49681 aaacgtcttt caaaccttct tctgttcttg agtcgcttgc gtttttatgc aagtcttttt
    49741 cgactgtatt gcgtaatgtt tcgctgtcgc cgaagatttc aaagatcgta tcatcggaac
    49801 caaatcctaa cgcacgaact aatacggtta aaggaatctt acgtgtacgg tcgatacgta
    49861 cataagagat gtcttttgca tcagtttcca tttctagcca agctccacgg ttagggataa
    49921 cagtagaacc gaatccttct tttccgtttt tgtcgacttt tccatggaaa tagacacctg
    49981 gtgaacggac taactgagaa acgattactc gctctgcccc gttgatgatg aatgttcctt
    50041 gttctgtcat taatgggaaa tcaccgaaga atacttcttg tgctttgatt tcgccagtct
    50101 cgcggtttgt caaacgcaat gttacatgta atggtgcaga gtagtttgca tcatgcgcac
    50161 gagcttctgc tactgtatac tttggttctt tcaattcata atctacaaat tccaaagata
    50221 gattgccatt gaagtcatcg attggcaaaa tgtcttcaaa catttctcta agtccctcat
    50281 ctaagaacca ttggtaagag tctgtttgaa tttcgatcaa atttggtaat tccaacactt
    50341 cactgattct tgcgaaactt ctacgttcgc gatgttttcc gtattttact acgtgtccag
    50401 ccaagctctt cacccctcta aaatgtcatt caaaaagact acccgatcaa ttgttacaat
    50461 aatatttcaa atattatttt taggtaacaa cagacatttt ttgcggcttt taggcaaaaa
    50521 aaaaccaaaa atagaacatt ctcttgaaac cttacttcgt gagcttctac ttttgtttcc
    50581 ttatattaaa gccgaaacgg acaatatttc gtttctgcct tttcttcatc agatagtttt
    50641 tgcaacttca tatactacac ttattttcta taaaagtcaa cacttcctgg cgtttcttct
    50701 gctttttcgc aacattttct taatgttttc aattttatgt tttcaattat ctgacaattc
    50761 ttaattgaca gaccttttta tctcggttat actttagaaa attcattttg tggaggaact
    50821 tctatgcgaa ccaacatcaa ccaaatcgca ccaacacaac tgaataactt ttactttagc
    50881 tactttagat aggtttgtat tcatcatgat ggatacaaac gttaaacgac agtttgtatc
    50941 tatgatggct aaagaacttt gtattgcagc aaaaagccac gtagacaaaa tgctaagtgg
    51001 ctgagcagtg aaaatacaaa gtaaaagtat caagcattct actttttcaa tttattttcc
    51061 gacttttcaa tccatttgga cattttgtct gaatggattt tttgtttccc aaaattaagg
    51121 aggaaaaaga aatgaaaaaa aatataatca tcggattagg cgctgccctg ctactagctt
    51181 taggaggttg cgcacaaaaa gacaattccg cagaggcatc agatggacaa aaaacaattg
    51241 gcgtattgca acttgtacaa cataactcgt tagatgctgc ctataaagga ttcaaagaag
    51301 gattagcaga agaagggtat aaagaaggcg ataatctaac aatcgattat caaaatgccc
    51361 aaaacaatca agacaacctg aaaagtatga gtgagaaact cgtcaaagaa gatcctgatt
    51421 tgctacttgg aatcgccact ccagcagcac aatctttatt aaatgaaaca accactatcc
    51481 cgatgactgt cacagcggtc acagacttgg aacaagccca tttagtcaca tccaatgaaa
    51541 aacctggcgg caatgtgact ggcacgacag atatggtgcc aatcgaaaaa caaatcggct
    51601 tactgttaaa tatcgtgcca gacgccaaaa caatcggcat catgtataac aacggtgaag
    51661 caaactctaa gatccaagct gatttggcag aaaaagaatt gaaaaaagcg ggcgtcaaag
    51721 tcaaagtact gacagcaaat tccacaaatg atgtccaaca agtgacgaaa agtctagcta
    51781 aagatgtaga tggaatctat attcctactg ataacacgtt cgcaagtgcg gcaagtgttg
    51841 tcggtgaagt cgcaaaagaa acaaaaacac ctgtcgttgc tggcgcagtt gatcaagtca
    51901 aagacggcgg tctagcaaca gtaggaatcg actacgaaag cttgggcaaa caaacaggga
    51961 tcatggcagc aaaaatctta gacggcaaga atccaagtga acttcctgtc gaaacagcga
    52021 atgatttgga actgtatgtc aataaagaca tggccaaagc attaggcatc aatccagaca
    52081 gcatcaaaga agctgaataa aacacaggag gattctcatg acaaatttgc tctctctttt
    52141 cttatcatct gcatcgcaag gggtcttatg ggccctactt gcgattggtg tatacgtcac
    52201 tttccgtatc ttggacattg ccgacttgac tgctgaagga agctttcctt taggtgcagg
    52261 gatcgcggca gtaagtatta caaatggcta tcatccattg acagcttgtc ttctaggctt
    52321 tttagggggt gcggcagcag ggcttgtttc cggactgctt cacacaaaat tgaaaattcc
    52381 cgcccttctt gcagggatca tcaccatgac tggtttatat tctgtcacat cccgtgtgat
    52441 gggtgcgccg aacatctctt tattgggtca gccaacgatc tttcattggg tcgagtcatt
    52501 cggcataaca aaagacaacg ctgtactgat cgtcggtctg ctttttgctt tgatcgttgt
    52561 atgtttactt gttttgttct tacgtacaga aacaggactt gctattcgtg caactggcga
    52621 taatctggca atgagcgaag caaacgggat caacacagat aatatgaaga tcatcggtta
    52681 tatgatttct aatggatgca ttgctctttc tggctcctta cttgcgcaaa acaacggctt
    52741 tgctgattta aattctggta ttggaacgat cgtaattgga cttgcttcta ttatcatcgc
    52801 agaagttttg ttacgtaacc agccactatt tctccgtctc ttgactgtcg ttttaggtgc
    52861 ggtcatttat cgttttattt tagcgttagt cttcgaactg aatgttgaac caagtgattc
    52921 gaaacttgct tctgctttcg tgctagtgat ctgtctttct ttcccgcaga tccagcaaaa
    52981 attccattta cctcaaaaaa agaaaggcgg tagtgtcaaa tgaatgacgt cttaacattg
    53041 gaaaatctcc atcatacctt tgaaaaagga acgatcaacg aaaatcatgt cctgaaaggc
    53101 attaatcttt ccttagatcc cggcgacttc gtgacgatca tcggtggaaa tggggctggt
    53161 aaatctactt tgctgaacag catcgccggt acattttctg tcgatcaggg tcaaatttta
    53221 ttgaatggaa aaaatatcac gaaaaaatct gttgtcgaac gttcgaaatc catcagtcgc
    53281 gtattccaag atcctaaact agggacagcc gttcgtttaa cggtagaaga aaatcttgct
    53341 cttgctatga aacgaggaaa aaaacgcggc ttcttccgtg gtgtcaaacc acaagaccga
    53401 acatttttca aagaccagct ggttcggcta aatttggggt tggaaaatcg tttatcctca
    53461 gaaatcggtt tgctgtctgg aggacaacgt caagctatta cgttattgat ggccacatta
    53521 cagcgtccag aactgatctt attagatgaa cacactgcag cgcttgaccc tgctacttcc
    53581 aaaacggtga tggaattgac agaaaagctg atcactgagc agaatctcac tgcttttatg
    53641 gtcactcatg acatggaaga cgcgatccgc tatgggagtc gactgatcat gctgcatcaa
    53701 ggaaggatcg tcgtagatat ccagcaagag gaaaaacagc agctcacagt gccccaatta
    53761 ttagcgttgt tcaaacaaaa cagcggcacg gcattaaaag acgatcaagt acttttatct
    53821 tcataatcag aaagaggatg ggacaaaaaa tgaacgccca ccctcttctt ttttctcttt
    53881 ccttctttcg tttttgcttt atgtatcaac cgttgtttct tcgaaaacta ttttgttctc
    53941 tccttcttcc tgatagaatg gatgggaaag gagcttgatg ccatgacaac gaaagacaag
    54001 gtacttcaat tattgaaaaa gacaccagat tttctttccg gagaaaagtt ggcacagcaa
    54061 ctggaagttt cccggacaag tatttggaaa gccatcaaag aattggaaaa ggcaggctat
    54121 cgttttgagc acgggcctac agggtatcgt tacctgccct ctgacgtact agatgcaaaa
    54181 gaaatccagc aagagctacg gtcgtttatt cctgatttga aagtacaaac gatgttgact
    54241 tctgaatcta cgatgaaaga tgccaaactg gcagcagctc aaggagattc tgtttcgaag
    54301 ttatttgttg ctgatacaca agaacaagct cgtggacgtt ttggacggcc ttttttcgct
    54361 gaacctggac gaggaatcta catgagtctc ttgctccatc cagacaaacg gtttgatgaa
    54421 ttgcctcaat atacgatcat cgcagccagt gcctgtgtga aagccattga atctgtaacc
    54481 ggtcgttctg cttcgatcaa atgggtcaat gatatctaca tggatggaaa aaaagtctgt
    54541 gggattttat ctgaagcagt cagtgatatg gaaagcggca ggatcagtca tatcatcatt
    54601 gggatcgggc ttaatttttc tgttcctcaa aatcattttc cagctgatct ccaagaaaaa
    54661 gccatctctg tctttcccga cggacaagct agtgtcacaa ggaatcagct aatcagcaaa
    54721 atctggcaag aattgttcac ttatctttct cacccagaaa aagcgttgga cttctataaa
    54781 gaaaaatcat tcgtattggg aaaaaccgtg tcgttcaatc aaaaaggaac gacttatgtc
    54841 ggtcttgcaa aagaaatcgg tgatcatggc gaactgatcg tccaaacaaa tgaacagaag
    54901 atgagtttgt cttctggcga aatcagtctc acatctatcc gtacgcaata attatcagat
    54961 ttcacaaaag gacacctgat ctcatacagt gtcctttttt ctgaattctc ttcaaaagac
    55021 tctccttatt ctatcagctg acagtttctc tatcctctag aaaaaggtat tctaaaaata
    55081 tctataaagc atggtacact ttctttaaat gaatcaatgc aaaaaacaag agagtcatca
    55141 gtttacacga tcaaaagaga tagcaggccg cttccttttt caatcttata gatagaccat
    55201 gggtaacgat aaaaacaaaa aagaacttgc gacttatttt gccgtaagtt cttttttagc
    55261 tagtgcctta atctcttaat cccaacaaac gtctcgcata gatcacctga ccagagtgca
    55321 tagcagcatc atcaataata gaaacgagac gcattcctcg tgtgactggt ggtgtccagt
    55381 tctcgtcaat tacttcatct agctcggaag gttccagcct tcttaagtaa gaaatagcaa
    55441 aatcagcagc tcctcgcaag tatcctagca gatcttctat ttgattagct tggatctgct
    55501 ttgcttgttc cagactatgc ttccaatctt gttcgttatc tgctatcgtc atcggaaacc
    55561 ggctttccca gtcttgacta tgccaaattg gagaagttcc tgctaaatca gcaatctgga
    55621 aatctaattc tcgagcggta tgccaagcca gccaagtcat tgatttgatc tgcggggctc
    55681 tatcttccac tggaaaacga ttggcctcct caatagagac gccttcaaac aaacgttcaa
    55741 accgatcgta tgcccgatcc acactctcgc atagcatatc taaataatcc atttattttc
    55801 ctccttttta tttatcctac ttactttttc ctaattctag gatacctact tatatgttac
    55861 tacacaccct tattttttac actttaacag tcaccatcat caatagtttg gagattgaaa
    55921 gaaaaagata gtgtcgaact atcaattgaa caagaagcaa caatatgaac gtttcattaa
    55981 tattctttaa ataaacaatg atttttcaaa cgaacagttt aagactagga gaaaaagagt
    56041 ataataggaa aatgtttgaa aggaaaggaa ggggaaaaat gaaaaaaagg gcaagtttac
    56101 tagtcctagt tgctttgttg ttcaatatct tttcgtatgg tgtggccgtt tcagcggaaa
    56161 catacattcc taaagaccct gggaatattt ataatgatct aactggtaac tatggcttgt
    56221 taggaattgc tagtcaattc cacgtatttg ctaaaaataa gacaactgtg aatgcacata
    56281 cagacggaaa tatagcaaca catgaacttg atgcaaaaaa taattttggt acagagcttc
    56341 atgaaggttt attgaaccaa gaaataaatt atatacaaaa aactgattct ttcgctaatt
    56401 cttcaggcat acctacaggt agcacaagaa tgaataaatt tgtcgttggc agtaacgtgg
    56461 atgtagcact tcaagatggt catcctacag tcaatggatc caggcttgat cacttaactc
    56521 cagatgattt ttataaagat cgtgctgggc atgaatatat tgatttcgag caggaatttg
    56581 ctaaattgaa tgtagcagca aatgatcttg caacgatcac tccagctaaa acatatactg
    56641 ctgcagattt tccagacatg aacaatcgga caatcgattt aactgattta tcagataccg
    56701 ggtttttatt ggtaaatatc gatgcagaag tgttaacgat gaatacgcca cttcaaatca
    56761 ttaatccaaa tgatcaagta gttgtattta atgtcatcaa cagttcttct gcattgaacg
    56821 tacagtcacc aatcaaatac aatgatcgtt caaatcatga aacagaagat ttttcggatg
    56881 cgaatatctc ttggaatttt ggaaatgaga tgactgattt gacaatcagt gctccttttc
    56941 aaggaacaat ccttgcgcct aatgctacta tccgcattac tcaaaaccaa gacggaacga
    57001 ttataggaac aaatgtgatt ttagatgcag ctaccaatcg ttgggatcct aacgaaatat
    57061 ttatcagcga caatggtaca gatacgacag acacaagcga cagtacagat acatctacca
    57121 caagtgatag ctcagattca tcaacttcaa gcgacagtac agatactacc tctaccacaa
    57181 gtgatagcac
//
LOCUS       DARBET010000010        51635 bp    DNA     linear   BCT 07-NOV-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4027
            SAMEA114569841-rid20112183.denovo.010, whole genome shotgun
            sequence.
ACCESSION   DARBET010000010 DARBET010000000
VERSION     DARBET010000010.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA114569841
            Sequence Read Archive: ERR12203666
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 51635)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 51635)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001972618.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001972618.1
            Genome Coverage       :: 132x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/07/2023 13:18:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,041
            CDSs (total)                      :: 2,975
            Genes (coding)                    :: 2,908
            CDSs (with protein)               :: 2,908
            Genes (RNA)                       :: 66
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 67
            CDSs (without protein)            :: 67
            Pseudo Genes (ambiguous residues) :: 0 of 67
            Pseudo Genes (frameshifted)       :: 26 of 67
            Pseudo Genes (incomplete)         :: 28 of 67
            Pseudo Genes (internal stop)      :: 20 of 67
            Pseudo Genes (multiple problems)  :: 6 of 67
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..51635
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA114569841-rid20112183.denovo.010"
                     /isolate="VRE4027"
                     /db_xref="taxon:1352"
                     /country="Denmark"
     gene            119..1045
                     /gene="cysK"
                     /locus_tag="SCJ86_000707"
     CDS             119..1045
                     /gene="cysK"
                     /locus_tag="SCJ86_000707"
                     /EC_number="2.5.1.47"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336979.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine synthase A"
                     /protein_id="HEG1168291.1"
                     /translation="MTAIYNSVTELIGKTPIVKLNKIVPEDSADVFVKLEFFNPGGSV
                     KDRIALSMIEKAEHDGLLKPGDTIIEPTSGNTGIGLSMVGVAKGYKVIIVMPETMSIE
                     RRLLMKGYGAELILTPGADGISGSIREAERLAKENGYFLPLQFENEANPLVHEKTTGP
                     EIHQAFGVNGLDAFVAGIGTGGTITGAGRELKRVYPKIELIGVEPAESAILEGKEAGP
                     HKIQGIGTGFVPKTLDTSVYDKVLSISGDEAMETAREVGRKEGILVGISSGAAIAAAL
                     KVAKELGKGKKVLAVVPDNGERYLSTALYQEI"
     gene            1281..1727
                     /locus_tag="SCJ86_000708"
     CDS             1281..1727
                     /locus_tag="SCJ86_000708"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289542.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional repressor"
                     /protein_id="HEG1168292.1"
                     /translation="MENKLVEEVIEKLKKENIRITPQRYAVLEYLIEHHSHPTADEIY
                     KALEHRFPNMSVATVYNNLRLFTSIGFVQEMKYGDASSRFDFSSKRHYHVICENCGKI
                     VDFHYPGLEDIEMAASRLTDFEINEHRLELYGLCPDCKESAKKAQM"
     gene            2000..3352
                     /locus_tag="SCJ86_000709"
     CDS             2000..3352
                     /locus_tag="SCJ86_000709"
                     /EC_number="7.1.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289543.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH oxidase"
                     /protein_id="HEG1168293.1"
                     /translation="MKVVVIGCTHAGTAAVKSILKNHPEAEVTVYERNDNISFLSCGI
                     ALYVGGVVKDPAGLFYSNPEELSSLGATVKMEHDVTDVDTENKTVTAKDLKTGETETV
                     SYDKLVMTTGSWPIIPPISGIDAKNVLLCKNYNQANEIIAQAKEAKRVVIVGGGYIGI
                     ELVEAFVESGKQVTLIDGLDRILNKYLDKPFTDILEKELTDRGVTLALGENVTEFITD
                     EEGKVKQVATPTDTFDTDMVILCVGFRPNTKLIEGKVDTLPNGAIKVNEYMQTSNPDI
                     FAAGDSAVVNYNPSGTQNYIPLATNAVRQGLLVGNNLTDHKMAYRGTQGTSGLYLFGW
                     TIGSTGVTKESATLNDLEVQATVFEDNYRPEFMPTTEKVMMELVYEKGTNRIVGAQFM
                     SKYDITQSANTMSLAVQNKMTVEDLALSDFFFQPHFDRPWNYLNLLAQAALGEIETAQ
                     "
     gene            3523..3810
                     /locus_tag="SCJ86_000710"
     CDS             3523..3810
                     /locus_tag="SCJ86_000710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289544.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEG1168294.1"
                     /translation="MNKEYFVQQDAFLEDYKSASKKLTINIDELADQVLSQLNETQEE
                     YYTMPANDAKDNVSHRFPFSRRIYSDENDGTLNTEYTYEGQPYVLETDDDL"
     gene            4036..4479
                     /locus_tag="SCJ86_000711"
     CDS             4036..4479
                     /locus_tag="SCJ86_000711"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289545.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HEG1168295.1"
                     /translation="MDKRQLLIEEYLDLANEIIHRNKPEMEKEFAGFTLNELEVIEHI
                     GKIPDPNVTKLANASYMTRGAISKLTKKLIARGIINTYQSEENKKEIYFQLTPIGQKI
                     RNRHEQLHQKWVQRDATVFENMSKEEFDTVFRFIGRYREFIRSSK"
     gene            complement(4485..5669)
                     /locus_tag="SCJ86_000712"
     CDS             complement(4485..5669)
                     /locus_tag="SCJ86_000712"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303796.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetracycline resistance MFS efflux pump"
                     /protein_id="HEG1168296.1"
                     /translation="MYSFTNKHSKVFGYVSVLFTGLGFTIVSPVLPFLTLPYSHSIHQ
                     QAFYITLLMSVYALAAFLSAPILGSLSDHFGRHPILIISLLGSSIGYLLFGLGNSIWM
                     LFLGRIIEGLTAGEISTLYAYFADITEPNERTKVFGWMGALVGIGTTLGPIIGGLLAE
                     LGNSVPIFIGALFTFLNAVYGYTFMLESLPIKKRSVDLSFSHVRPFHQLQQLFKISSV
                     IPLLTAGFAVWLAAGSLQSIFSQFSIDAFQWKAGLVGLSFSLIGILDTVSQLFIMPRL
                     LKKFSEQQITRIGMFSEILAYLFITLSGILLLPILFLFGIICYGFGDSIFTPVFNGQL
                     SNSVSDNQQGLVMGGTQSIQSLSRVIGPLIAGQLYAAAPFLPTVFGFLLMIFAYYHYN
                     KK"
     gene            5937..6635
                     /locus_tag="SCJ86_000713"
     CDS             5937..6635
                     /locus_tag="SCJ86_000713"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002372388.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DnaD domain protein"
                     /protein_id="HEG1168297.1"
                     /translation="MLNLENYLSAGQTVISNLLLKNYHRIGLQSEEFLFVLQLHMAQL
                     EGDTFPDLQMIAMDMGIKQDQIFQILDRLVTNGFIKIETTIVNGKKADRYNLYPIYDL
                     LGEYLKTQEKKYEEKTQEKEIQSVYQLFEQEFGRPLSSIEFQRIGQWLEEDHYQPEIL
                     KLALREAVLNQAYSFNYVDRILLSWERKNLRTKQQVEEDQKRRKQQLLQREVEQSAQQ
                     EELPKVSLKNWLEE"
     gene            6642..7319
                     /gene="nth"
                     /locus_tag="SCJ86_000714"
     CDS             6642..7319
                     /gene="nth"
                     /locus_tag="SCJ86_000714"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339330.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease III"
                     /protein_id="HEG1168298.1"
                     /translation="MLSKQKTMEALETMYGMFPEAHGELKHNNPFELLIAVILSAQAT
                     DVSVNKATPDLFASFPTPDALAEASIDEIILKIKTIGLYRNKAKNIKACAQQLIERFD
                     GQVPTSREELMSLPGVGRKTANVVLGDAFGIPAIAVDTHVERVSKRLRICKLDATVME
                     VEETLMRKVPQELWVKTHHTLIFFGRYHCTARNPKCEVCPLLSICQDGKNRMRLKEKA
                     LKKKPRL"
     gene            complement(7445..7978)
                     /locus_tag="SCJ86_000715"
     CDS             complement(7445..7978)
                     /locus_tag="SCJ86_000715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289611.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEG1168299.1"
                     /translation="MVLNNFLKEGFILSYLIVCLIIIICLHHLFLSKTIPRYSKNKKA
                     EKFCLLLNKFTLIAPILAFIIFSVLLSTTLKGKFMERSSHAMILTFLWLLFTRIYIFL
                     MSLKPPKSISLCLVINGIFLLSLIIFITPLDRYVTYLYNPLEYWTYFIGILEGIIFYI
                     GYFPNKNNNFYFYRNKL"
     gene            complement(8275..10656)
                     /locus_tag="SCJ86_000716"
     CDS             complement(8275..10656)
                     /locus_tag="SCJ86_000716"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PBP1A family penicillin-binding protein"
                     /protein_id="HEG1168300.1"
                     /translation="MANEQTRSSRRQKQPTPKKSVKKNSGKGSGKSSGTHKKGLFIKI
                     LLGILSFFCILFLAGVGLFWYYAKDAPELTDKKLDATVSSKLYTQDGELFEDLGAEKR
                     EKISANELPKTLEDAIVSVEDRRFYKHIGVDPIRIIGSALSNFTSGGLQGGSTLTQQL
                     IKLSFFSTSAEDQTLKRKAQEAWMAVRLEQKKSKQEILTYYVNKVYMSNGLYGMETAS
                     EMYFGKKLSELSLPQTALLAGMPQAPSAYDPYVYPDQAKKRRDTVLYTMLQNEKISQT
                     EYDQAVNVPVTDGLQELTQSDDNTKIVDNYVKEVINEVQEKTDKNVYTDGLEIYTNLD
                     LDAQKKLYDIVNTDQYVSYPDDEMQVASTLIDTNTGKVKAQIGGRHIAEDVTLGNNLA
                     VNTSRDFGSTMKPVTDYGPAFEYLKYSTGKTITDAPYNYEGTSTPVGNWDNQYMGTIT
                     LRQALYLSRNVPAVKLFNEVGSDKVASFLKNLGIEYSTIHQSNAISSNTEEQDGTKYG
                     ASSLKMAAAYAAFANGGTYYKPQYVNKIVFQDGTEETYEPDGKTAMSPETAYMITDIL
                     KDTITEGTGTNAQIAGLYQAGKTGTSNYTDDEYAKLGISSGVYPDILFAGYTPNYSIS
                     VWTGYNKKMTPVTSESSHVASDVYRELMQYVSANVTNTDWEMPSGLIRVGGELYYKDQ
                     YTARSNAITPSTTIPSSSYVQTPGSSTTETTTQSSSSTSQSESTAESSKESTTAETSE
                     PASSTTVPSSSSEESSTPSSSAPPASSSEPATSGADAANDHTPSSSTSASGNR"
     gene            complement(10646..11293)
                     /gene="recU"
                     /locus_tag="SCJ86_000717"
     CDS             complement(10646..11293)
                     /gene="recU"
                     /locus_tag="SCJ86_000717"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction resolvase RecU"
                     /protein_id="HEG1168301.1"
                     /translation="MVLRYPNGQPYYKNASPVEKIQEKRNQITEFGNRGMRFEEAINE
                     SNKYYLSRGLAVIHKKPTPIQIVKVDYPKRSAAVIKEAYFTQASTTDYNGVYRGFYLD
                     FEAKETKNKTSFPFKNFHAHQIEHMKACLEQNGVCFVLLWFSSLKRCFYLNSVHLIAY
                     WDAQKEKGRKSLPLSLIEELGIEIKTGISPRIPYLDAVDFYLSTLSIEGESKNGK"
     gene            11357..11896
                     /locus_tag="SCJ86_000718"
     CDS             11357..11896
                     /locus_tag="SCJ86_000718"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327933.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1273 domain-containing protein"
                     /protein_id="HEG1168302.1"
                     /translation="MDKSKVIYVSGYRSFELGVFKENDPKIQVVKNVLKKELHQLAEE
                     GLEWVLVSGNLGVELWAVQIVAELKEEYPELSLGILYPFSDFGNNWNEGNQEKKQLAE
                     QLADYVEAVSHQPYQSPSQLKNHTRFILEHTVGCLLIYDEEFPGKTKYFFEDAQNYQA
                     DHPYEIRLISMDDLQNFSE"
     gene            11986..12405
                     /gene="gpsB"
                     /locus_tag="SCJ86_000719"
     CDS             11986..12405
                     /gene="gpsB"
                     /locus_tag="SCJ86_000719"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295835.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division regulator GpsB"
                     /protein_id="HEG1168303.1"
                     /translation="MANLVYSPKDILQQEFKTKMRGYDPVEVDEFLDNIIKDYEAYSK
                     ELLALQEENDRLSAKVAQLSKTQGAAQTRVQQTEAPKSAAVTNFDILKRLSNLEREVF
                     GKKLDQQASAVKPAQPNPNNYTNADTSLDDNEKTRQF"
     gene            12427..12794
                     /gene="rnpB"
                     /locus_tag="SCJ86_000720"
     ncRNA           12427..12794
                     /ncRNA_class="RNase_P_RNA"
                     /gene="rnpB"
                     /locus_tag="SCJ86_000720"
                     /product="RNase P RNA component class B"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00011"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00011"
     gene            13003..14169
                     /locus_tag="SCJ86_000721"
     CDS             13003..14169
                     /locus_tag="SCJ86_000721"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent RNA methyltransferase"
                     /protein_id="HEG1168304.1"
                     /translation="MEKNRKFNLVATAASGLEALVGKELRDLGIECQVENGRARFEGT
                     METIATANLWLRTADRVKIVVGEFDAYTFDELFENVKALPWEDFLPLDAAFPVAGKSI
                     KSKLYSTPDCQAITKKAIVERLRTYYHRPASVPLTETGAHFQLEVALLKDHVMVTLDT
                     TGPSLFKRGYRLEKGGAPLKENMAAALIMLTNWRKDRPFYDPVCGSGTLCIEAALIGH
                     NIAPGFNREFACESWDWFSQEIMENVRSAAEEKADYDIELDITGSDINGRMIEIAKAN
                     AEEIGLGSSITFKQQAVKDFKTDKEYGVIVANPPYGERLGEEETVRKLYKEMGDVFRP
                     LKTWSKYILTSDLAFEEFYGQKATKKRKLYNGALRTDLFQYWGTRPPRKKEKDE"
     gene            14215..15711
                     /locus_tag="SCJ86_000722"
     CDS             14215..15711
                     /locus_tag="SCJ86_000722"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738715.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxypeptidase M32"
                     /protein_id="HEG1168305.1"
                     /translation="MKEKEFLQEVKEINLLQQALGILDWDTQTGMPEKASAYRSEVDS
                     YLYSIYFSKKIGPKIKEAIRYFSEHPEELSEVGTAVFAKVKEEYDLEHRVPEDLMRKY
                     TAAVSSAHGQWQKARETQQFSDFQHALNQNIELTKQLIPYWRKDEKTNYDVLLNQYEP
                     GMTVEILDKVFDQLKEGILSIRRTLQEKGTEPETDFLNRRMTKKQQKRFVVKVIEQLG
                     YDFSRGRLDDTVHPFMTGINPNDARITTRWNETDFKMAVFGVIHEAGHGMYEQDIDDK
                     YAYTPVYEGTSMGIHESQSLFNEIIIGSSRSFWKKQYPFFQECAEGTFDDIPFDKFYR
                     SLNHTKSSLVRIEADSLTYPLHIIIRYEIEKMIFNEGVDVAELPEIWNQKYEEYLGIR
                     PTNDVEGILQDVHWSGASFGYFPSYALGYMYAAQLLHSLQQEIDVENVLASDDYSPIK
                     NWMSEHIHQYGASRKPNQLIMDATHEPLNPQYLIDYMKDLYFNVYQVK"
     gene            15792..16475
                     /locus_tag="SCJ86_000723"
     CDS             15792..16475
                     /locus_tag="SCJ86_000723"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002372396.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing protein"
                     /protein_id="HEG1168306.1"
                     /translation="MDKLQVIADYSMDQLAHDQTGHGSDHTKRVVKLAEKILETEPQA
                     DRFVTLAAAYLHDTIDDKVVKNENEAKQQLRVFLRSLPIVEEQISMIFSIIENMSFSK
                     NLSEAAELSLEGKIVQDADRIEALGAIGILRTAYFGGGHGHPIFDPELYPQTFKDKEN
                     YRKGTTVINHFYEKLFLLSDKMNTDYGYEEAKRRETFMREFLEEFFLEWSIDDENFTP
                     DNWIKNNKK"
     gene            17120..18310
                     /locus_tag="SCJ86_000724"
     CDS             17120..18310
                     /locus_tag="SCJ86_000724"
                     /EC_number="2.3.1.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine C-acetyltransferase"
                     /protein_id="HEG1168307.1"
                     /translation="MSSKELTMYLEKELDQLKEKGLYNTIDILESENGACIIVDGKKM
                     INLASNNYLGFANREELKKACIEATETYGVGAGAVRTINGSLKIHQQLEEKIAEFKGT
                     EAAIAFQSGFNCNMGAISAVMTKEDAILSDELNHASIIDGCRLSGAKIIRIKHQDMKD
                     LEKKAKEAIESKKYKKIMYITDGVFSMDGDIARLPEIIPIVEKYGLITYVDDAHGSGV
                     TGKGAGTVKHFGLSDKIDMQMGTLSKAIGVVGGYVAGSKTLIDWLKARSRPFLFSTSL
                     TPGAAAAALASITLMQEHPELVEKVWENANYFKEELKKVGYNIGMSETPITPVILGDE
                     KVTQTFSKKLIEHGIYAKPIVYPTVPLGTGRIRNMPTAEHTREMLDEAVAVYQKIGKE
                     MEII"
     gene            18412..19365
                     /locus_tag="SCJ86_000725"
     CDS             18412..19365
                     /locus_tag="SCJ86_000725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338267.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-threonine 3-dehydrogenase"
                     /protein_id="HEG1168308.1"
                     /translation="MKKVLVTGCLGQIGSELITRLRQELGSENVIATDIRMPEVIEKN
                     DIFEILDVMDYEKMRSIAEQYQVDTLIHLAALLSAVAEAKPQLAWNLNVTGLVNALEV
                     AREFQLKFFTPSSIGAFGPNTPKDHTPQDTLQRPTTMYGVTKVAGELLCDYYYVKYGV
                     DTRGVRFPGLISYATLPGGGTTDYAVDIYYSAIKEGKYTSFIDQGTSMDMMYMPDAID
                     AIIKLMNADPDRLVHRNAFNISAMSFDPEEIKASIQKIIPDFEMDYDVDPVRQSIAES
                     WPNSLDVSCAQEEWGFSPKYDLDVMTKDMLEKLKEKLVETV"
     gene            19434..20783
                     /locus_tag="SCJ86_000726"
     CDS             19434..20783
                     /locus_tag="SCJ86_000726"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HEG1168309.1"
                     /translation="MEEYQATPVKEVKIENELKRTMGFFTALSTVMGTVIGAGVFFKA
                     ASVAEVTGSASLHMFSWFLGGMISVCAGLTGAELAAAIPETGGMIKYIERIYGNTAAF
                     LLGWAQVVIYFPANVAALSIIFGTQFVNLFGLSQSMIVPVAVTAAVSILLINFLGSKA
                     GGAFQSITLVCKLIPLFVIVIFGLFRQEGVDFQLFPIQAGENLSFFSALGAGLLATMF
                     AYDGWIHVGNISGELKKPAKDLPKAISLGIIGIMIVYLLVNAVFLRTASIDGVAGNSN
                     AASDVAKMIFGGFGGRLVTVGILISVYGTINGYTLTGMRLPYVMAKENNLPFSKLFAK
                     LHDKTKVPVAAGILELVIAIGMMMIGGFDTLTDMLIFVIWIFYTMVFVGVILLRKKEP
                     DLFRPYKVPMYPFIPLVAIIGGTFIVSSTLITQTFLASMGIVLTLAGIPIYLYLKRR"
     gene            complement(20966..21289)
                     /locus_tag="SCJ86_000727"
     CDS             complement(20966..21289)
                     /locus_tag="SCJ86_000727"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289192.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEG1168310.1"
                     /translation="MLNMYFVFGVPIFLLFLYATIAYVRKRTTIHYLGFILLIISGFM
                     LVFNLQTWQQALLEMDKMTPHALSKVLGYPVYLIWLPIFISGCLVLLNIYRGVRRIVQ
                     LRKSK"
     gene            21418..22581
                     /locus_tag="SCJ86_000728"
     CDS             21418..22581
                     /locus_tag="SCJ86_000728"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631795.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RDD family protein"
                     /protein_id="HEG1168311.1"
                     /translation="MASYIEPIRFALIVFPFLALAISSVFFIYEYRKYGTFLVTRAVI
                     LYSFVFYLLCAYFLVILPLPAKETVAQMTGPKMELHLFASWQHFRDNTVLVLSEPSTY
                     LPAMKQSVFLEPAFNVLLLIPFGIYLRYYFRLSFMKTMIASFCLSLFFELTQLSGLYF
                     IYPRPYRLFDVNDLVTNTLGGIIGFVVTPLFTFMLPTRRRMDELSYEKGKTVSLTRRL
                     VAYLIDWTFLSLINLVAAIFFKIAFDSGAVTSHIWWLLLEVGVYFMIVPFITNGWTLG
                     KKIVRIRLVEEDRERISLKALMIRYGYLYFLFYGLSWLGQLLQQIMNSTTNTVLQMSA
                     VFISLGVLLLQGIFILNILLSFLRKKRILFYEKASHTYTVSTIGQTKDDDSRN"
     gene            22651..24000
                     /locus_tag="SCJ86_000729"
     CDS             22651..24000
                     /locus_tag="SCJ86_000729"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309341.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HEG1168312.1"
                     /translation="MNNFDIFRFKPFINEALTAKGFKQPTEVQERLIPLIAKGKNIIG
                     QSQTGTGKTHTFLLPLFEKINAEKEEVQVVITAPSRELAAQIYEAATQIAKFSTPEIR
                     VSNFVGGTDKQRQLNRLHHQQPQVVVGTPGRILDMMNEQALKIHTAFAFVVDEADMTL
                     DMGFLNEVDQIASRLPEKLQFLVFSATIPEKLRPFLRKYMENPIIEEIKPKAVISEDI
                     ENWLISTKGKDENQMIYQLLTIGHPYLAIVFANTKQHVDEIADYLKNQGLKVAKIHGD
                     ITPRERKRVMRQVQNLEYQYVVATDLAARGIDIEGVSHVINAEIPEDLDFFIHRVGRT
                     GRNGLKGTAITLYSPNDEQAIDAIEKLGVSFEPKEIKNGEIVKTYDRNRRAKREKSKD
                     VLDPTLIGLVKKKKKKIKPGYKKKIDWAIAEKNKKERKIERRQQSRTARKNKKNSHK"
     gene            complement(24045..24476)
                     /locus_tag="SCJ86_000730"
     CDS             complement(24045..24476)
                     /locus_tag="SCJ86_000730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEG1168313.1"
                     /translation="MKKIVGADEWIRAASCFLRYQVFVLEQGIPAELEFDGKDIPQQT
                     YYLFLDQKRPVATLRYEKKTPTCFKPERFCVAKEYRGKGLGGQLLEAAEKRAVSEGCQ
                     TSYVVAEMTAVDFYEKQGYHTCTEPFIEDEVLCIGMKKSLA"
     gene            24864..27506
                     /gene="alaS"
                     /locus_tag="SCJ86_000731"
     CDS             24864..27506
                     /gene="alaS"
                     /locus_tag="SCJ86_000731"
                     /EC_number="6.1.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349591.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alanine--tRNA ligase"
                     /protein_id="HEG1168314.1"
                     /translation="MKQLSSAQVRQMFLDFFQSKGHTIEPSASLIPVNDPTLLWINSG
                     VATLKKYFDGSVVPENPRITNAQKSIRTNDIENVGKTARHHTMFEMLGNFSIGDYFKN
                     EAIHWAWEFLTDPKWMAFDPEKLYVTVYPKDTEAKRIWHEEVGLPLDHIIDVEDNFWD
                     IGAGPCGPDTEIFYDRGEAYNDLAEDDPENYPGGENERYLEIWNLVFSEFNHKPDNTF
                     EPLPHKNIDTGMGLERMVSIVQDASTNFETDLFMPIIHAVEKLSKQVKYGENPVTDIS
                     YKVIADHIRALSFAIGDGALPSNEGRGYVLRRLLRRAVMHGKKLGIEQAFLYRLVPVV
                     GEIMVSYYPEVLQQKEFIEKVIRTEEERFNETINEGLEILNQVIQEVKNENGTTLAGK
                     DIFKLYDTYGFPVELTEEVAEDEGLKVDHAGFEKEMDAQRERARSARSTEKSMGVQSA
                     LLTDIKVESKFIGYTEQESDSQLLVIVKGESLLNEVAEGEAQMIFDQTPFYAEMGGQV
                     ADQGWIMNEVGEIVAVVTDVQKAPNGQFMHTVEVKAPMSEGQTYHLLIDQSMRNRIIK
                     NHTATHLLHKALKEVLGEHANQAGSLVAPGHLRFDFTHFGQITSDELAEMERIVNEKI
                     WEAIPVETIETDIDTAKNMGAMALFGEKYGHDVRVVNIGGWSIELCGGTHVANTENIG
                     IFKIVSESGIGAGVRRIEAVTSKEAYELLEEEEKQLKQVATIVKSPQLKEVVSKAEQL
                     QQQVRELQKENEQLASKLANQQAGDVFKDPETINGKTIIAAQVKVKDMNQLRQLADQW
                     KQKNISDILVLANEQEGKVSLLAAMTKESNEAGLKAGDLIKAIAPKVGGGGGGRPDMA
                     QAGGKNPAGIPDALKAAKEWVAQQ"
     gene            27706..28302
                     /locus_tag="SCJ86_000732"
     CDS             27706..28302
                     /locus_tag="SCJ86_000732"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723497.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ECF transporter S component"
                     /protein_id="HEG1168315.1"
                     /translation="MNSRNKTFRLVLRAILLAIIIVQAMVPWLGFIPLGFISLTIIHI
                     TVIIAAVVLGPKDGMVIGLFWGIATIVRAYAMPTTPFDTLVFTNPIISVVPRVLVGLV
                     AGLVFHWIYQKNKSITISSAFAGVLGSLVNTVLVLGFMGLFYTGATADAYGVDSALLF
                     KTLAGVAAINGIPEAIGAGIITPLLAKALFAATPLKPE"
     gene            28424..29572
                     /locus_tag="SCJ86_000733"
     CDS             28424..29572
                     /locus_tag="SCJ86_000733"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311124.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation transporter"
                     /protein_id="HEG1168316.1"
                     /translation="MAKKQEEKKKQIPQQRTKKGVLAGILGLVTNLILFVSKLMIGLF
                     SGSVSIMADAVNSLSDTASSILTLIGFKIAAKPADQEHPYGHERFEYISGLFVSIIIT
                     YVGFQFLESSAEKIIHPENVSLTPIVFFVLIISIFLKFAQGKMYQVIAQSIDSETLRA
                     TATDSYNDVFTTLAVLLSAAIEWVTGWRIDGYIGFALALYILYSGLTMLRNFVYELLG
                     SRPTPAEIEEMEEHLNACDDILGYHDLLVHNYGPNKRFASVHIEVDESMNLNVAHKII
                     DLIEKDFKKSLDVELVCHLDPMPVDNEKYYNILRQLKQILLETGTGLNIHDFRVIHQG
                     KTLQFDVVVPEKFQFVDTELERILRSQIEEKIGDYQLEITFDHNYLLY"
     gene            complement(29610..30023)
                     /locus_tag="SCJ86_000734"
     CDS             complement(29610..30023)
                     /locus_tag="SCJ86_000734"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295847.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease HI family protein"
                     /protein_id="HEG1168317.1"
                     /translation="MLKVYIDASTKGNPGPSGGGILIVYQGKQEQLSIPLSIGTNHQA
                     EFEVFLKTLEFLKKNDLQNETIFCFSDSKTLVSTVDKNATKNELFLPYLQRIQELLSE
                     FSLLILQWLPENKNKGADNLARQALHKQLKEKKTL"
     gene            30102..30533
                     /locus_tag="SCJ86_000735"
     CDS             30102..30533
                     /locus_tag="SCJ86_000735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323283.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EbsA family protein"
                     /protein_id="HEG1168318.1"
                     /translation="MKKNIRWQPELAHTIIYWSLTLIILFFSLILSLENTGPYWKSNF
                     VLGIFLVFLFLGLRRSFILKSNSVIIRYAAFWKDREISVGQIRSLTPTNTGVQMMLQD
                     EKAPSVFMMRKKDQVVLFDHFEKNGPKSESIPYPYTKKRKS"
     gene            complement(30632..30865)
                     /locus_tag="SCJ86_000736"
     CDS             complement(30632..30865)
                     /locus_tag="SCJ86_000736"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346560.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cold shock domain-containing protein"
                     /protein_id="HEG1168319.1"
                     /translation="MTTGIVKWFDNKKGYGFISYDDTEEIFVHFTAIEEEGFKTLEEN
                     QVVEFEIIEGNRGTQAAHVKKGNVQTTDTSMHE"
     gene            31048..32718
                     /locus_tag="SCJ86_000737"
     CDS             31048..32718
                     /locus_tag="SCJ86_000737"
                     /EC_number="6.3.4.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008268812.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate--tetrahydrofolate ligase"
                     /protein_id="HEG1168320.1"
                     /translation="MKNDLQISQETELEPIVEVASKLDLKEDDLELYGKYKAKINFSG
                     INRSKDNAEGHLILVTSINPTPAGEGKSTITVGLGDALNKINKKSVIALREPSLGPVM
                     GIKGGAAGGGYAQVLPMEDINLHFTGDMHAITTANNALSALLDNHIHQGNELNIDARR
                     VIWKRVVDLNDRELRKVIVGLGGPIQGVPREDGFDITVASEIMAILCLAADLEDLKAR
                     LARIVVGYTFDRQPVTAGDLKAEGALALLLKDAIKPNLVQTIYGTPAFVHGGPFANIA
                     HGCNSILATKTALRLADYVVTEAGFGADLGGEKFLDIKVPNLKKAPDAVVIVATVRAL
                     KMHGGMKKDELKNENLDALKVGFANLKRHIRNMEQYQLPVIVAINEFVTDTDNELTLL
                     EHLCENQGILAKRASVWADGAEGGVDLAKAVVRLIDRKEANYKPLYRLEETIQEKTET
                     IVKKIYGGNGVVFSKKAQKQIEEFTKNGWDKLPICMAKTQYSFSDDPSLLGAPEDFTI
                     TIREIIPKLGAGFLVALTGDVMTMPGLPKKPAALNMDVTNDGEVLGLF"
     gene            32865..33251
                     /locus_tag="SCJ86_000738"
     CDS             32865..33251
                     /locus_tag="SCJ86_000738"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEG1168321.1"
                     /translation="MKTTFIRKTGFYGMGSSIQLRINNEKRSVLSHEQKITLDLEVPF
                     TMQVTFYWLKSPVYTVKEAKPAYVITMNYLILQIYPFLFLATGLGAVFLQNLFFSFFA
                     VIVLIAFFFYIKNRAYLIKEENYGKL"
     gene            33238..33936
                     /locus_tag="SCJ86_000739"
     CDS             33238..33936
                     /locus_tag="SCJ86_000739"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376865.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CBS domain-containing protein"
                     /protein_id="HEG1168322.1"
                     /translation="MANSDLFLISFNRIEKWMRDEMGNARNMGFTELVRRLAQRKQLM
                     IRKYEDDLLQLAQLRNAIVHDRIAVDFVIAEPNDWTVKRIQLIEQELIQPETVLPRFA
                     KRVTGFEQDLPLLELLKIVAEKRYSQFPLYNKGKFVALITLRNIGFWLAKESQKGPVD
                     LTNKRALDLIIQNGKYTNYHFVPAKTHIYEVEAMFREQGTLEAVLITKDGNPDGNLLG
                     IVRPRDIYKQIEKD"
     gene            33941..34405
                     /locus_tag="SCJ86_000740"
     CDS             33941..34405
                     /locus_tag="SCJ86_000740"
                     /EC_number="3.4.23.36"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299710.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptidase II"
                     /protein_id="HEG1168323.1"
                     /translation="MLIIYWIFSALLVGVDQLVKWWITDNLALGETKNLIPGILSLNH
                     IRNTGAAWSMLEGKMWFFTIVTLIAVVVILTLMIKNREKGNRWFMIGLSLILAGAIGN
                     FIDRIRLGYVVDMFQTDFMSFPIFNVADVTLVCGVICMLIYIILDEKDQRKK"
     gene            34402..35313
                     /locus_tag="SCJ86_000741"
     CDS             34402..35313
                     /locus_tag="SCJ86_000741"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349590.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RluA family pseudouridine synthase"
                     /protein_id="HEG1168324.1"
                     /translation="MTELKTTIHHESGRIDKVLTNLFTDYSRSQIQLWLKNGAVSVNG
                     QPVKANYKVKQGDEITVLIPEPETLDVVPENIPLTIVYEDEDVAVINKPQGMVVHPSA
                     GHTHGTMVNALMYHIKDLSTINGVIRPGIVHRIDKDTSGLLMVAKNDQAHESLAQQLK
                     DKTSLRKYVALVHGEIPHEKGTINAPIGRSKENRKMQAIREDGKSAVTHFTVLERFEG
                     FTLVELKLETGRTHQIRVHMKYIGYPLAGDPVYGPKKTLKGNGQFLHAQMLGFTHPTT
                     NEKMIFEAPLPEVFEKTLEKLRKNIAF"
     gene            35548..36087
                     /gene="pyrR"
                     /locus_tag="SCJ86_000742"
     CDS             35548..36087
                     /gene="pyrR"
                     /locus_tag="SCJ86_000742"
                     /EC_number="2.4.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295862.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional pyr operon transcriptional
                     regulator/uracil phosphoribosyltransferase PyrR"
                     /protein_id="HEG1168325.1"
                     /translation="MQAKEVVDQVTMKRALTRITYEIIERNHSIQDIVLVGIKTRGIY
                     IASRIAERLKQLEDIDIPVGELDITLYRDDKKENPEEPELHSSDIPVSLEGKEVILID
                     DVLYTGRTIRAAMDAVMDFGRPRKISLAVLVDRGHRELPIRADYVGKNIPTSRAEEIL
                     VEMQELDGQDRIMILKEED"
     gene            36101..37384
                     /locus_tag="SCJ86_000743"
     CDS             36101..37384
                     /locus_tag="SCJ86_000743"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005879677.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uracil permease"
                     /protein_id="HEG1168326.1"
                     /translation="MSEKKGFHNDDVVLDIHDRPTPGHWVGLSLQHLFTMFGATVLVP
                     ILVGIDPGIALVSSGLGTLVYLFTTKGKIPAYLGSSFAFIAAMQLLMKTDGYPAIAQG
                     AVTTGLVYLIVALIIKKIGSAWLDKILPPIVVGPVIMVIGLGLAANAATNAMYNQGTY
                     DFKYIAVALITLGLTIFFNMWLRGFLGLIPILLGIISGYIVALLFGIVDTKPIVDAAW
                     FAVPNFEIPFVQYEPKLYLGAITTMAPIAFVTMTEHIGHLMVLNKLTKRNFFEEPGLH
                     KTLTGDGLAQIVAAFVGGPPVTSYGENIGVLAITRVHSVFVIGGAAVFAVILGFVGKL
                     SALILSIPGPVISGISFILFGVIAASGMKILIENKIDFDKKKNLLIASVILVVGIGGL
                     VFEVGTFTLSAMALATVLGIILNLILPETSRSEEN"
     gene            37397..38323
                     /locus_tag="SCJ86_000744"
     CDS             37397..38323
                     /locus_tag="SCJ86_000744"
                     /EC_number="2.1.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322463.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate carbamoyltransferase catalytic
                     subunit"
                     /protein_id="HEG1168327.1"
                     /translation="MIIKSERISLKHLLTVEALTDQEVMGLIRRGQEFKQGANWTPQK
                     RQYFATNLFFENSTRTHKSFDVAEKKLGLEVIEFEASTSSVQKGETLYDTVLTMSALG
                     VDVAVIRHGDENYYDELIQSKTIQCSIINGGDGSGQHPTQCLLDLMTIYEEFGHFEGL
                     KVAIVGDITHSRVAKSNMQMLKRLGAQVFFSGPREWYDDEFEVYGHYMPLDELLDQVD
                     VMMMLRVQHERHDGKESFSKEGYHQEHGLTVERAKKLQKHAIIMHPAPVNRDVELADS
                     LVEGLQSRIVQQMSNGVFVRMAILEAVLNGKA"
     gene            38342..39622
                     /locus_tag="SCJ86_000745"
     CDS             38342..39622
                     /locus_tag="SCJ86_000745"
                     /EC_number="3.5.2.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339315.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroorotase"
                     /protein_id="HEG1168328.1"
                     /translation="MKTLIKNGQINTRKNMTTPAEIWIEDGRIKAIGTGFSEAEFDEV
                     FDAKGQLITPGLVDVHVHLREPGFTYKETIEAGTRSAARGGFTTVCAMPNLNPVPDTA
                     EKLRQVYDIIRKDAVVKVLQYAPITENLRSEKLVDQEALIEEGAFAFTNDGVGVQTAG
                     TMYLAMKEAAKNNKALVAHTEDESLLFGGVMHAGKKAEELGLPGILSVTESSQIARDL
                     LLAEATGVHYHVCHVSTKESVRVIRDAKKAGIHVTAEVSPHHLILIDEDIPEDFGFWK
                     MNPPLRGREDREALIEGLLDGTIDCIATDHAPHGLEEKSQSFMKSPFGIVGSETAFQL
                     IYTHFVETGRFTLEQVINWLAVKPAEIFGLNAGTLTVGAPADVAVFDITQTCTIDKED
                     FLSKGENTPFIGWKVKGETQMTFVNGKLVWQKGE"
     gene            39627..40703
                     /gene="carA"
                     /locus_tag="SCJ86_000746"
     CDS             39627..40703
                     /gene="carA"
                     /locus_tag="SCJ86_000746"
                     /EC_number="6.3.5.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345088.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine-hydrolyzing carbamoyl-phosphate
                     synthase small subunit"
                     /protein_id="HEG1168329.1"
                     /translation="MERLLILEDGTVFEGKAFGADASVFGEIVFTTSMTGYQETITDQ
                     SFNGQIITFTYPMVGNYGVNRDDYESISPTCKGVVVKEHARVASNWRNQMTLDSFLKQ
                     KGIPGISGIDTRALTRKIREVGTMKASIVDAGDSFEHAFDQLKATVLATNQVQQVSTN
                     KPYPSPGTGRKVIVIDFGLKHSILRELSKRNCNLTVLPYNTDAETILALSPDGVMLTN
                     GPGDPKDVPEAIEMIKKIQGKVPIFGICLGHQLFALANGADTYKMKFGHRGLNHPVRE
                     VATGRIDFTSQNHGYAVDEATIDPEKLIVTHVEVNDGTVEGVKHRDYPAFSVQFHPDA
                     APGPHDALHLFDEFMEMMDAGKEQ"
     gene            40708..43893
                     /gene="carB"
                     /locus_tag="SCJ86_000747"
     CDS             40708..43893
                     /gene="carB"
                     /locus_tag="SCJ86_000747"
                     /EC_number="6.3.5.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001126118.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbamoyl-phosphate synthase large subunit"
                     /protein_id="HEG1168330.1"
                     /translation="MPKRTDIKKIMVIGSGPIIIGQAAEFDYAGTQACLALKEEGYEV
                     VLVNSNPATIMTDKEVADQVYIEPITLEFVSRILRKERPDALLPTLGGQTGLNMAMEL
                     AASGILEELNIELLGTKLSAIDQAEDRDLFKQLMKELNQPIPESEIVNTVEEAVDFAN
                     KIGYPVIVRPAFTLGGTGGGMCENEEELRIVSENGLSLSPATQCLIERSIAGFKEIEY
                     EVMRDSADNAIVVCNMENFDPVGIHTGDSIVFAPSQTLSDHEYQMLRDASLSIIRALK
                     IEGGCNVQLALDPHSFNYYVIEVNPRVSRSSALASKATGYPIAKLAAKIALGLTLDEM
                     KNPVTGTTFAEFEPALDYVVAKIPRWPFDKFENGERLLGTQMKATGEVMAIGRNIEES
                     LLKAVRSLEIGTHHIELPELADITDDDLIQKVVRAQDDRLFYVAEAIRRGYTIEELAD
                     LTKIDLFFLDKLLHIVEIEQQLKQQPLDDKILKTAKQNGFTDKKVAELWEINEEAVRQ
                     MRKENDLIPVYKMVDTCAAEFESKTPYFYSSYEYENESSRSEKPSILVLGSGPIRIGQ
                     GVEFDYATVHSVKAIQQAGYEAIIMNSNPETVSTDFSISDKLYFEPLTFEDVMNVIEL
                     EQPIGVIVQFGGQTAINLAEPLTAAGVKILGTTIEDLDRAENRDLFEQALKELDIPQP
                     LGDTATSKEEAVVIASRIGYPVLVRPSYVLGGRAMEIVENQRDLEDYMEHAVKASPEH
                     PVLVDRYLLGGECEVDAICDGETVLIPGIMEHIERAGVHSGDSMAVYPPQTLSESIKK
                     TIEDYTIRLALGLNCVGMMNIQFVIHENEVYVIEVNPRASRTVPFLSKVTGIPMAQIA
                     TKAILGEKLKTLGYENGLYPETKEVHVKAPVFSFTKLQKVDTYLGPEMKSTGEVMGSD
                     RQLDKALYKAFEASGLHLPEYGAVLFTIADETKEEALELAKRFTEIGYSLIGTKHTAA
                     YFEKNGLVVETVAKISEEAAEKNVVDLIRDGKTQVVVNTIDKDRGNASKDGFIIRREA
                     VEHGIPLFTSLDTADAIVRVMESRAFSTNPI"
     gene            43947..44723
                     /locus_tag="SCJ86_000748"
     CDS             43947..44723
                     /locus_tag="SCJ86_000748"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337699.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroorotate dehydrogenase electron transfer
                     subunit"
                     /protein_id="HEG1168331.1"
                     /translation="MKQEMMEIVQQKQLAPKIFEMTLKGDLVKEMQQPGQFLHILVPR
                     ADLLLRRPISLNHIDHETNTCRIIYRVEGEGTRVFSTLSAGDHLDVMGPLGNGFDLSN
                     LHTGDEVFIIGGGIGVPPLYELSKQLVEKGIKPTHFLGFATHEVVYYEKEFNRLGETR
                     IATDDGTYGMHGNVGNLLLGTKTEPTAVFACGSNGLLKTVEQLFSSHQNVQLSLESRM
                     ACGIGACYACVCHTPEDETGTKSVKVCDEGPIFKIGEVII"
     gene            44720..45652
                     /locus_tag="SCJ86_000749"
     CDS             44720..45652
                     /locus_tag="SCJ86_000749"
                     /EC_number="1.3.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309316.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroorotate dehydrogenase"
                     /protein_id="HEG1168332.1"
                     /translation="MNENPLAVKLPGLELKNPIMPASGCFGFGKEYGKYYDLNQLGSI
                     MVKATTPNARFGNPTPRVAETPSGMLNAIGLQNPGLDVVMHKLLPELEEYNELPIIAN
                     VAGACEEDYVEVCSKIGEAPNVKAIELNISCPNVKHGGIAFGTDSDVAFQLTQAVKKV
                     SSVPVYVKLSPNVTDIVPIAQAIEAGGADGFTMINTLLGMRIDLKTRKPILANQTGGL
                     SGPAIKPVAIRLIHQVASISSLPIIGMGGVQTVDDVLEMFMAGASAVAIGTANFTDPY
                     ICPKLIKELPVRMSELGIESLERLIKEVREERER"
     gene            45649..46365
                     /gene="pyrF"
                     /locus_tag="SCJ86_000750"
     CDS             45649..46365
                     /gene="pyrF"
                     /locus_tag="SCJ86_000750"
                     /EC_number="4.1.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="orotidine-5'-phosphate decarboxylase"
                     /protein_id="HEG1168333.1"
                     /translation="MNQLDQRPIIALDFSTRQEVEDFLRFFPKEEKLFVKIGMELFYQ
                     EGPEIVRYLKEAGHNVFLDLKLHDIPNTVEKAMRGLAKLGVDLTCVHAAGGIRMMEAA
                     MRGLEEGTPEGGKRPLLLAITQLTSTSEEEMHADQLIEVSLEKSVIHYASCAKKAGLD
                     GVVSSAWEVEAIKEMAGDEFVCLTPGIRPEGTVAGDQTRVVTPSQAKKIGSTFIVIGR
                     PITQSTNPYKAYQTIQTEWS"
     gene            46372..47061
                     /locus_tag="SCJ86_000751"
     CDS             46372..47061
                     /locus_tag="SCJ86_000751"
                     /EC_number="2.4.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010737541.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="orotate phosphoribosyltransferase"
                     /protein_id="HEG1168334.1"
                     /translation="MNVEQSIAKDLLEIEAVFLNPSNPFTWASGIKSPIYCDNRITMS
                     YPKVRKEIAKGLASKIKEAYPEVQVIAGTATAGIPHAAWVAEILDLPMVYIRSKAKDH
                     GKGNQIEGRIFEGQKMVVIEDLISTGGSVLEAAEAAKREGADILGVAAIFTYELPKGK
                     ANFEKAEIPLMTLTNYSVLIEAALEDRYIDEQELTLLKEWKKDPENWQIEARLMKIEK
                     SDLCLQNHQLE"
     gene            47034..47303
                     /locus_tag="SCJ86_000752"
     CDS             47034..47303
                     /locus_tag="SCJ86_000752"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299722.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEG1168335.1"
                     /translation="MSPESSIGIAKIQKLIARSIFSSGMKRNFYRDQFLIFLKFIFLR
                     LVSRKKRIISSLFSIIYRFSLFFGKGAVSKMVKSGNVANISYLMI"
     gene            47341..47982
                     /locus_tag="SCJ86_000753"
     CDS             47341..47982
                     /locus_tag="SCJ86_000753"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324830.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing protein"
                     /protein_id="HEG1168336.1"
                     /translation="MGLNEFILGLNNLETITRAPGFFKYTEHTVAAHSYRVASIAQVL
                     GDIEEANGQPIDWKLLYEKSLNHDYTERFIGDIKTPVKYASKELRGMLQTVEEKMTDE
                     FINQEIPAEFQEVYRRRLFEGKDETVEGELLAISDKVDLLYESFDEIIKNNPEKVYKE
                     MFIEAVTTIKSFEHRPSVQYFFKEIFPELLNQEFYGKEAFLETVSTIVKKEEK"
     gene            48025..48717
                     /locus_tag="SCJ86_000754"
     CDS             48025..48717
                     /locus_tag="SCJ86_000754"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376854.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbonic anhydrase family protein"
                     /protein_id="HEG1168337.1"
                     /translation="MDVEWGYSGDKGPENWASLCDWFARGAEFPLQSPIHLTKGEETK
                     IEGDTLSFHYQKQRFTDKEFKNTIHLVPFDQLSYVEFKKERYYLTDIHFHLPSEHIIN
                     GKQEDIEFHFVHMNSKKENLVVGVMYQLMVQEGWLYNQENGESWNLEKHEHWVNPDVF
                     FPEQKSHFHYIGSLTTPPTSGPIQWFVMDHVGKLNQEFLLPFDGQYARPNNRPIQPLN
                     GREIYYFEEFEQ"
     gene            48714..49616
                     /locus_tag="SCJ86_000755"
     CDS             48714..49616
                     /locus_tag="SCJ86_000755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326365.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HEG1168338.1"
                     /translation="MNIQQMKYVVAVANNGSFREAAKKMFVSQPSLSAGIKELETELG
                     ITLFTRTNRGAYLTEEGQDFLNRAEKILVQLDTLETHYLTSEKRDNFSIAAQHYDFLG
                     PLTAQLLEEFKDEIKQFRIMETTTARVIEEVKEQHSEIGIIYMNEHNQAGIRRYLQQG
                     ELSGRSIGKFKTHIFLRQGHPLAEHAEITKEELLSYPQVRFTQDGNNFPYFYEDLIEI
                     PDQETVIYTSDRGTLMNIVLETDAYASGSGIVIGGIREHLRLIPLAEGELNEFYIIYS
                     AKRPLSEIAQRFIKELTRLLDQQS"
     gene            complement(49774..51480)
                     /gene="efbA"
                     /locus_tag="SCJ86_000756"
     CDS             complement(49774..51480)
                     /gene="efbA"
                     /locus_tag="SCJ86_000756"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629407.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fibronectin-binding protein EfbA"
                     /protein_id="HEG1168339.1"
                     /translation="MSFDGVFTHAMVNELRETLLSGRISKIHQPYENEIVLVIRSRGK
                     NHRLLLSAHPSYARVQITQIDYQNPDNPPNFVMMLRKYLDGAILEDIEQIENDRVIHF
                     HFAKRNELGDLQNIILIVELMGRHSTIVLVNRETGKILDAIKHIGSSQNTYRSLLPGV
                     EYVAPPKQEVLNPFSSEKEKIFQRLSQTELDPKAIQRQFQGIGFDIAQELTKRLLERP
                     NEKMVVWDEFFSAISHQPIPTFYETENKDFFTPIAYQVLSEQASAVTAYPTLSQLLDS
                     YYHEKAEKDRAKQQGGELIRKIENELKRNKNKLKKREQTLKESENAENYRRNGELLTT
                     FLTQVPRGAKEVVLPNYYEEDRPIKIALDPALTPNQNAQKYFHRYQKLKNAVKLIGEQ
                     IQEAKDEIQYLESVLSQLEIAGPMDIEAIKEELTSEGYLKKKNLKKQKRKKPSQPDQY
                     FSSDGTLILVGKNNLQNDQLTMKTAKKTDYWLHAKNIPGSHVIIKSDKPSDETITEAA
                     ELAAYFSKYRYSAQVPVDLVQVKHIRKPNGAKPGYVIYENQKTIIVTPEEEKITAMKQ
                     NR"
BASE COUNT        16894 a         8983 c        11096 g        14662 t
ORIGIN      
        1 attaccatat atggtatatc tttgtctttt ttttgctagt caaagacgaa tcactcgcca
       61 taaatgaaca gattgtatta tgataagtaa gaagagcaaa gacaaggagg aattgaagat
      121 gacagctatc tataactcag tcaccgaatt gattggcaaa acgccaatcg tcaaattaaa
      181 taaaatcgta ccagaagatt cagcagatgt ttttgtaaag cttgagtttt ttaatccagg
      241 aggaagtgta aaagatcgta ttgctttaag tatgatagaa aaagcagaac acgatggtct
      301 actgaaacca ggtgatacta tcattgaacc tacttctgga aatacaggaa tcggattgtc
      361 gatggtagga gttgcaaaag gatataaagt aatcatcgtg atgcctgaga cgatgagtat
      421 cgaaagacgt ttattgatga aagggtacgg cgcagagttg attttgactc ctggtgcgga
      481 tggtatttct ggatcaatca gagaagcaga acgtttggca aaagaaaacg gctacttttt
      541 accattacaa tttgaaaatg aagcaaatcc tcttgtgcat gagaagacga ctggtcctga
      601 gatacatcaa gcatttggag taaatggctt ggacgcgttt gttgcaggta tcggtacagg
      661 aggaacgatc actggcgctg gccgagaatt aaaacgtgtg tatccgaaaa tcgaattaat
      721 cggggtagaa ccagcagaat ctgctatttt agaagggaaa gaagcgggac ctcacaaaat
      781 ccaaggaatc ggtacaggtt ttgttcctaa aacactggat acttctgtgt atgataaagt
      841 cctttctatt agtggagacg aagcgatgga aacagctaga gaagttgggc gaaaagaagg
      901 gattttagta ggcatctcat ctggcgctgc aattgctgcc gcgctaaaag tcgcaaaaga
      961 attaggaaaa ggcaagaagg tattagctgt ggttcccgac aatggtgaac gttatttatc
     1021 tacagccctt taccaagaga tataaggcac ttggtttttc tccagatagg tttatgtaaa
     1081 aaacttttaa agatgaaaag aacacacaaa aatttatgat gagaaaaatt atttgaatag
     1141 agacgtagaa tagtgaacgc gactgtttta cgtctttttt cattaaaata caataaaatt
     1201 gaattttctt ttcatgttaa gtggatttta aaatgtaaaa atagtatgat aagacgagaa
     1261 taacaacgtg gggtgtttac atggaaaata agttagtcga agaggttatc gaaaagttaa
     1321 aaaaagagaa cattcgaatc acacctcaac ggtacgcggt tttagaatat ttgattgaac
     1381 atcattccca tccaacggct gatgagattt ataaagcact tgaacatcgg tttccaaata
     1441 tgagtgttgc gactgtttac aataaccttc gcttatttac ttcgattgga tttgttcaag
     1501 aaatgaagta cggcgatgct tctagccgat ttgatttcag ttcaaagcgt cattatcatg
     1561 taatttgtga aaactgcggt aagattgttg attttcatta cccagggctt gaagatatag
     1621 aaatggctgc aagcagactg actgattttg agattaatga acatcggttg gaattgtatg
     1681 gactctgtcc ggattgtaaa gagtcagcaa aaaaggcgca aatgtaaaaa cagtttctac
     1741 aagtgttata cctaaaaaaa gctagctgtg taaaaaaaac gctagctttt ttattttttt
     1801 gtcttttatt cacaatctta gatataaaag tgaatattat taaaataaga atcttgtaga
     1861 tattgggttc ttatgcttga aaaggaatga aactacaaac aaacctttgt gaattcacaa
     1921 gcattaaact gttgtatccg ttttatttct gttatagtag ttttgtcgaa ggacaaaatg
     1981 ttaatcggag ggaccataaa tgaaagtagt agtaattggc tgtactcatg caggtactgc
     2041 agctgtaaaa agtattttaa aaaatcatcc agaagcagaa gtgacagtat atgaaagaaa
     2101 tgacaatatt tctttcttat cttgcggtat cgcactttat gtcggcggag tagtaaagga
     2161 cccagcaggc ttgttctact caaatcctga agaattatct tcattaggag caacagttaa
     2221 aatggaacac gatgtaacag acgtcgatac agaaaataaa acagtaacgg caaaagatct
     2281 gaaaacagga gaaacagaaa cggtttctta tgataaatta gtgatgacaa ctggttcatg
     2341 gcctattatc ccaccgatta gtggaatcga tgcgaaaaat gtactcctat gcaaaaacta
     2401 caatcaagca aatgaaatta ttgcacaagc gaaagaagca aaacgtgtag taattgttgg
     2461 tggtggatat atcggtattg aattagtaga agcttttgtc gaatctggaa aacaagtgac
     2521 attgattgat ggattagatc gcatcttgaa taaatatcta gataaaccat tcacagatat
     2581 cttagaaaaa gaattgacag accgcggtgt tactcttgcg ctaggcgaaa atgttactga
     2641 atttatcaca gatgaagaag gaaaagtgaa acaagtagct acaccaacag atacatttga
     2701 tacagatatg gtcattttat gtgtgggctt ccgtccaaat acaaaactga tcgaaggaaa
     2761 agtagataca ctgccaaacg gggcaatcaa agtgaatgaa tacatgcaga caagtaatcc
     2821 agacattttt gcagctggag actctgcagt agtcaactac aatccaagtg gcacgcaaaa
     2881 ctatattcct ttggcaacaa atgccgttcg tcaaggtttg cttgtgggaa ataacttgac
     2941 agatcataaa atggcgtatc gtggtactca aggaacttca ggactttacc tatttggctg
     3001 gacgatcggt tcgacaggtg tgaccaaaga aagcgcaact ttgaacgatc tagaagtcca
     3061 agcgactgta tttgaagata attatcgtcc agagttcatg ccaactactg aaaaagtaat
     3121 gatggagcta gtctatgaaa aaggaacgaa tcgtatcgta ggtgctcagt tcatgtccaa
     3181 atacgacatt acacaatcag caaataccat gtctttagct gttcaaaata aaatgactgt
     3241 agaagatctc gcactttcag atttcttctt ccaaccacat tttgaccgcc catggaatta
     3301 cttgaaccta cttgcacaag cagcgctagg cgaaatcgaa actgcacaat aaagaaaaag
     3361 ggttgtgaca caattttgtc acgaccctct gtcttttttt gtgaaaagta caattagttg
     3421 tctttcttcg aattttttgt cacaataagc agcaaagata aagctgttac ataaaagaac
     3481 aagcagcttt atttgatttt gaaaagaaag gtgagaaaga agatgaataa agaatatttt
     3541 gtccaacaag atgcattttt agaagattat aaaagtgctt cgaagaaatt aacgatcaat
     3601 attgatgagt tggcagatca agtactctct cagttgaatg aaacgcaaga ggaatattac
     3661 acgatgccag caaatgacgc caaagataat gtttctcatc gttttccttt cagtagacgc
     3721 atctactcag atgaaaatga cggcacattg aatacagagt acacatatga aggtcagcca
     3781 tatgtgctag aaactgatga tgatctttaa agacagtttt tgaaaaggcc gctcatcctt
     3841 aagtgatgac aaaaaatcat cacaattacc gatgactgtc tctttactat cttttaatca
     3901 gaaaaagggg ttatgacaag ctttgtcaac cccttttttt cttggtttca gattgggttt
     3961 tcctacaaaa tatttttatc tttgttgaat aggaaacaaa aaaacaatat aatgaaagaa
     4021 aaagaaggtg tagaaatgga taaaagacaa ctgctaatag aagaatatct ggatttagct
     4081 aatgagatta ttcatcgtaa taaaccagaa atggaaaaag aatttgcagg atttactcta
     4141 aatgaactag aagtgattga acatatcggg aaaatccctg atcctaacgt gacaaaatta
     4201 gcgaatgcta gttacatgac tagaggagcc atcagtaagc taacaaaaaa actgatagct
     4261 agagggatca taaacactta tcaaagcgaa gagaataaaa aagaaatcta ttttcaattg
     4321 acgccaatcg gacaaaaaat aaggaataga cacgagcaac tccatcaaaa atgggtacag
     4381 cgagatgcta ctgtatttga aaatatgtca aaagaagaat ttgacacagt atttcgtttt
     4441 atcggtcgat acagagaatt tatacgaagt agcaaatgaa gctactattt tttattgtag
     4501 tgataatagg caaaaatcat aagcaaaaat ccgaataccg tcggaagaaa tggtgctgca
     4561 gcataaagct gtcctgcgat tagcgggccg atgacgcgcg atagagattg tatactttgg
     4621 gtaccgccca ttacaagacc ttgctggtta tctgagacag aattggatag ttggccattg
     4681 aatacaggag taaaaataga atcaccgaaa ccataacaga taatcccaaa taaaaacaaa
     4741 atcggcagta gaagaattcc agataatgtg atgaatagat aggcgaggat ttcactgaac
     4801 atccctattc ttgtgatctg ctgttctgaa aattttttga gcaatcgagg cataatgaac
     4861 aattgcgaga cagtatccaa gattccaatc aaagagaagc ttaaaccaac caacccagcc
     4921 ttccactgaa aggcgtcaat cgaaaattga gaaaagatag attgaagaga gccagctgcc
     4981 aaccaaacag caaaacctgc tgtcaataaa ggaataacgg atgaaatctt gaataattgt
     5041 tgtaattggt gaaaaggtct tacatgggag aaacttaaat ctactgaacg tttttttatt
     5101 ggaaggcttt ctagcataaa tgtgtagcca taaacggcat ttaagaaagt aaagagtgct
     5161 ccaataaata taggaacaga attccccaac tcagctaata gaccccctat aataggaccc
     5221 agcgttgttc ctataccgac taaagcaccc atccatccaa aaactttggt tctttcatta
     5281 ggttccgtga tgtcggcaaa gtaagcatat aaggtggaga tttctcctgc cgtcaatcct
     5341 tcaatgatac gaccaaggaa caacatccat atcgaatttc ctaaaccaaa aagcaaatat
     5401 ccgatacttg atcctaataa actgataata aggatcggat gacgcccaaa atgatcactt
     5461 aagctaccga gaatgggggc agataagaag gcagctagag cataaacaga catgagcaaa
     5521 gtgatataga aagcttgctg atgtatggag tgagaataag gcagcgttaa aaacggaagt
     5581 acaggactga cgatagtaaa tcctaatcca gtgaataaga cagagacgta tccaaaaact
     5641 ttagaatgtt tatttgtgaa actgtacatg taaaaactcc tttatgcaat aatgttaact
     5701 tggaaacaaa aatagtgtat agtaattttg tttccttgtc aacattctaa ggaacagcga
     5761 aatctactta atttatttat tccgaaaagt gaagatacgc atcaaaaaca tgaacaaaca
     5821 aaaatcaagt agacgacatt gtaaatctga ccgatcagtg aagtaaatag aaaagaaacc
     5881 tgttcttgct tcccatgttt tttttacgta tactgtgatt gagcggagga gaaatcatgt
     5941 tgaatttaga aaattactta agcgctggac aaacagtcat atctaatctt ttacttaaaa
     6001 attatcatag gatcggtcta caatctgaag aatttttatt tgttctacag ttgcatatgg
     6061 cacagcttga aggagatact ttccctgatt tgcagatgat tgctatggat atgggaatca
     6121 aacaagatca gatttttcaa atactggatc gtctcgtaac aaatggattt atcaagatag
     6181 aaacgacgat tgtcaacggt aaaaaagcag atcgatataa tctttacccc atctatgatc
     6241 tattaggtga gtatttaaaa acacaagaaa aaaaatacga agaaaaaaca caagaaaaag
     6301 aaatccagtc agtgtatcaa ttatttgaac aagaatttgg acgcccgctt tcttcgatcg
     6361 agttccagcg gatcggacaa tggctagaag aagaccatta tcagccggaa attttgaagc
     6421 tagcactgcg ggaagccgta ttaaatcagg cttatagttt taattacgtt gaccgcattt
     6481 tgctatcgtg ggaaagaaaa aatctacgta caaaacaaca agtggaagaa gaccaaaaac
     6541 gaagaaaaca gcagcttctt caaagagaag tagaacagtc agctcaacaa gaagaattgc
     6601 cgaaagtctc actgaaaaat tggttggagg agtagcggaa tatgttaagc aaacaaaaaa
     6661 caatggaagc tctagaaacg atgtatggaa tgtttcccga agcacatgga gaactcaagc
     6721 acaataatcc atttgagtta ttgattgcag tgatcttaag tgcccaggcg acagatgtat
     6781 cagtgaataa agcaacgcca gatttgtttg cctcttttcc gacacccgat gcgttagcag
     6841 aagcttcaat cgatgaaatc attcttaaaa taaaaacgat tggtttgtat cgaaataaag
     6901 caaaaaacat caaagcgtgt gcacagcagc tgattgaacg tttcgatggg caagtgccaa
     6961 cgtcaagaga agaattgatg tctcttcctg gtgtaggcag aaagacagca aatgtcgtat
     7021 tgggggatgc attcgggata ccagcaatcg cagtagatac ccacgtggaa cgtgtctcaa
     7081 aacgcctgag gatctgcaaa ctagatgcta cggtcatgga agtagaagaa acactgatgc
     7141 ggaaagtacc acaagaatta tgggtaaaga cacatcatac actgattttt tttggtagat
     7201 atcattgcac ggcaagaaat cccaagtgcg aggtatgtcc tttgctatca atatgtcaag
     7261 atgggaaaaa tcggatgcgt ctaaaagaaa aggcgctaaa aaagaaacca cgcctgtaga
     7321 cgtggtttct ttaattgcaa ataaatgtat gaattatctt gttattatgg agaaaagctg
     7381 tttcgtttat cttttttgca tgaaaaagta aatgtacata ctcagtagct aggatggtaa
     7441 cttattacaa tttattgcga taaaaataaa agttgttatt tttattggga aaatacccga
     7501 tgtagaaaat aatgccttcc aatataccta taaaataagt ccagtattcc aaaggattat
     7561 ataaataagt gacgtatcta tctaaaggtg taataaaaat gataagtgat aataaaaaaa
     7621 taccatttat aactaaacat aaactaatgg atttgggcgg tttcaggctc atcagaaaaa
     7681 tatagattct ggtgaacaac aaccataaaa aagtgagaat cattgcatga gagcttcttt
     7741 ccataaattt tcctttaaga gtagtgctta aaagtacaga aaaaataata aatgcaagga
     7801 taggagcaat taaagtaaat ttatttaata ataaacaaaa tttttcagct tttttattct
     7861 tggaataacg aggtattgtt ttcgataaaa aaagatggtg cagacaaatt atgataatta
     7921 agcatactat cagataactc aaaatgaagc cttctttcaa aaaattattc aaaaccatat
     7981 attgtatttt ttatttgata ataacaatgg gattattaag aagtggcact aaataaaaga
     8041 ctagtgtgta tttagccgat ttaattataa acaagtaaat ggataaagta tatgtaatga
     8101 taaaagaaaa atggattttc taatttttaa gctaaatgtt agatcacaca tgtatatgct
     8161 ggatagtctt ataaaaaatc atgtaataca gcttggtaca tgatttttat ttgtagctgt
     8221 tttttacata gacacaaaaa agaaaccacg cttgtagacg tggtttcttt tttactatct
     8281 gtttccagaa gctgaggtgc tgctggatgg tgtatggtca tttgcagcat ccgctccgct
     8341 agttgctggc tcactggaag aagcaggagg agcagatgaa gacggtgtcg agctttcttc
     8401 ggatgaagaa gaaggaaccg ttgttgaaga cgctggctcg cttgtttctg ctgtcgtgct
     8461 ttcttttgag gactcagcag ttgattcgct ttgactagta gagctagagc tttgagtcgt
     8521 tgtctctgtt gttgagcttc caggagtctg gacatagctg gaagatggaa tagttgttga
     8581 tggtgtaatt gcattcgatc tagctgtata ttgatctttg tagtacagtt caccgcccac
     8641 acgaattaat ccgcttggca tctcccagtc tgtatttgtc acgttagctg aaacatactg
     8701 cataagctcg cgataaacat cagaggctac gtgtgacgat tctgaagtga caggtgtcat
     8761 ttttttattg taacctgtcc acacggaaat cgaataattt ggcgtatagc ccgcaaatag
     8821 gatatctgga tacaccccag aagaaatacc aagttttgca tactcgtcat ctgtgtaatt
     8881 ggatgttccg gtcttaccgg cttgatataa cccagcgatt tgcgcattcg taccagttcc
     8941 ttcagtgatc gtatctttta aaatgtctgt gatcatataa gctgtttctg gagacatggc
     9001 tgtttttcca tctggctcat aagtttcttc tgttccgtct tggaagacga ttttattaac
     9061 atattgaggt ttgtaataag tccctccatt ggcgaaagcc gcataggctg ctgccatttt
     9121 taaggaagaa gctccgtatt ttgtcccatc ttgttcttcc gtattactag agatcgcatt
     9181 cgattgatgg atcgtactgt attcgatccc aagatttttt aagaaagaag ccactttatc
     9241 tgaaccgact tcattgaata atttgactgc tggaacatta cgggataagt aaagtgcttg
     9301 gcgtaaagta atggtgccca tatattgatt atcccagttt cctacaggag tcgaagtccc
     9361 ttcatagtta tacggtgcgt cagtaatagt ttttccagta gaatatttca aatattcaaa
     9421 agctggtccg taatctgtga caggtttcat cgtagaacca aaatcacggg aagtattcac
     9481 tgctaagttg ttgcctaaag ttacgtcttc tgcgatgtga cgtccgccaa tctgggcttt
     9541 tactttcccg gtattggtgt cgataagggt ggaggcaact tgcatctcgt cgtctgggta
     9601 agagacatac tgatccgtgt tgacgatatc atatagtttc ttttgggcgt ccagatccaa
     9661 attcgtataa atctctaaac cgtctgtgta gacattctta tctgtttttt cttgtacttc
     9721 attgatgact tctttgacat aattatcgac gatcttagta ttgtcatcgg attgagttag
     9781 ttcttgcaga ccatccgtta ccggtacatt aacagcttga tcatattctg tttgagagat
     9841 tttttcgttt tgaagcatcg tatacaaaac agtatctcgt cttttcttcg cctgatctgg
     9901 ataaacataa ggatcatagg cggaaggggc ttgaggcatc ccagcaagca atgctgtttg
     9961 cggtaaagaa agttctgata atttcttacc aaagtacatc tcagaagctg tctccatccc
    10021 gtacaagccg tttgacatat acactttatt tacgtagtag gtcaagatct cttgtttcga
    10081 ttttttttgt tccaatctta cagccatcca cgcttcttgg gccttgcgtt tcagagtctg
    10141 atcctcggca ctggtggaga agaaggaaag tttgatcaat tgctgtgtca aagtacttcc
    10201 gccttgcagt ccgccagagg taaagttaga gagggcagaa ccgatgatcc gaatagggtc
    10261 aaccccgatg tgtttataaa aacggcgatc ttctaccgac acgatcgcat cttctaatgt
    10321 tttcggtaat tcattcgcag aaattttttc acgcttttct gcgcctaaat cttcgaatag
    10381 ttcgccatct tgtgtatata gtttggaaga tacggtagcg tctaattttt tatccgtcaa
    10441 ttcgggcgca tcttttgcgt aataccagaa aagtcctaca cctgctaaaa agaggataca
    10501 gaaaaatgag agtattccta aaaggatctt gataaataat ccttttttat gtgttccaga
    10561 ggactttcca gaacctttgc cagaattctt cttcacactc tttttaggag taggctgttt
    10621 ctgtcgtcta gagcttctag tttgttcatt tgccattctt gctttctcct tcaatagata
    10681 atgttgataa atagaagtca accgcgtcca aataaggaat ccgcggagaa ataccagttt
    10741 tgatctctat tcctagttct tcgatcaaag ataaaggtag tgatttgcgt cctttttcct
    10801 tttgggcgtc ccagtaagca atcaaatgaa cactattcaa ataaaaacag cgcttcaaag
    10861 acgaaaacca taacaagaca aagcagaccc cattttgttc gaggcatgct ttcatatgtt
    10921 cgatttgatg ggcatggaag tttttaaagg gaaaagatgt tttgttcttg gtttcttttg
    10981 cttcaaagtc caagtaaaag cctcgataga cgccattata gtctgtagtt gaggcttggg
    11041 taaagtaggc ttccttgatc acagcagcac ttctcttagg atagtctact ttcacgattt
    11101 ggataggggt aggcttttta tggataaccg ccaacccgcg ggaaagataa tatttattac
    11161 tttcattgat tgcttcttca aagcgcatcc cccgattgcc gaattcagtg atctgatttc
    11221 ttttttcttg aattttttcg acgggagagg cattcttata ataaggttgg ccatttggat
    11281 agcgcaatac catctttaat cacactccta ggccacatta tagcaaaaaa gcggatagaa
    11341 agaaaaggaa tctgagatgg ataagtcaaa agtaatctat gtgtctgggt atcgaagttt
    11401 cgaactcggt gtttttaaag aaaatgatcc taaaatccaa gttgttaaaa atgttttaaa
    11461 aaaagaactc catcagttag cagaagaagg gctggaatgg gtacttgtca gtgggaattt
    11521 gggcgtcgaa ctatgggctg tgcaaatagt agcggaattg aaagaagaat atccggaact
    11581 atctcttggt attttgtatc ctttttctga ttttggtaat aactggaatg aagggaacca
    11641 agaaaaaaaa caattagcag aacaattagc agattatgtc gaagctgtta gccatcaacc
    11701 ctaccaatca cctagtcagc taaagaatca cactcgtttt atacttgagc atacagtggg
    11761 ctgtctgctg atctatgatg aagaattccc tggaaaaacg aagtattttt ttgaagatgc
    11821 acagaattac caagcagatc atccctacga aattcggctg atcagtatgg acgatcttca
    11881 aaattttagt gaataatcga tattgtgttt gatttatcaa cttttttcct atacaatgaa
    11941 aaaggtagaa atgttattac gtaaatgaaa actagaggtg taaatatggc gaatttggtt
    12001 tatagtccta aagacatttt acaacaagaa ttcaaaacaa aaatgcgcgg ctatgatccc
    12061 gttgaagttg atgaattctt agacaatatc atcaaagatt atgaagctta cagcaaagaa
    12121 ctccttgctt tacaagaaga aaacgatcga ttgagtgcaa aagtagctca attatctaaa
    12181 actcaaggag cagctcaaac acgcgtgcaa caaacagagg caccaaagag cgcagcagtg
    12241 accaattttg atatcttaaa acgcttgtca aatcttgaac gtgaagtatt tggtaaaaag
    12301 cttgatcaac aagcaagtgc agtcaaacca gcacaaccta atccaaataa ctacacaaat
    12361 gcagacacaa gcttagatga taatgaaaaa acacgtcaat tttaattttt gattaatgaa
    12421 tttgcggttt tcgggtaatc gcggtctgac ttgatcaggc tgaggaaagt ccatgctcgc
    12481 acaggctgtg atgcctgtag tgttcgtgct tagcgaaacc ataagctaag gtactctttg
    12541 gagtaacggc aggaaaacaa gctaaggctt cggctatgct taagtatcct tgaaagtgcc
    12601 acagtgacga agcggctggg gaaacccatc cggtggaacg cggtaaaccc ctcgagcgag
    12661 caacccaaac aatggtaggg gcgctttttg tctggaagaa gaacaggaca aaaaggcagt
    12721 ataactgcag atagatggtt accgctttgt tttttctccc tggaaaagca aagtacagaa
    12781 catggcttat agaaaaccgc aaacatcagg gaattaggtc gtgacaaaag taatgcgtta
    12841 taagaaacaa gaaaaacatt tgaaagctat ttttcaatac ttcagatgaa tcgttcttat
    12901 tacaaaacaa acttttgtat caccggttat tcggaatttg agtaaaagtg tggggtagga
    12961 ctgatgtccc acctcctttt tttataaata aggagattgt gtatggaaaa aaacaggaag
    13021 ttcaacctcg tcgcaactgc ggcaagcggg ctagaagcac tggttggtaa agaattaaga
    13081 gatcttggaa tcgaatgcca agttgagaac ggacgtgcac gttttgaagg aacaatggaa
    13141 acgatcgcta cagctaattt atggttacgt acagcagatc gagtaaaaat cgtggttggg
    13201 gaattcgatg cgtatacgtt tgatgaattg tttgaaaacg taaaagcatt gccttgggaa
    13261 gattttcttc cactagatgc agcttttcct gtagctggaa aatcgatcaa gtcgaaacta
    13321 tatagcacac ctgattgcca agcgatcaca aaaaaagcaa tcgttgaacg tctccgtacc
    13381 tattatcacc gtccagcttc tgtaccactg acggaaactg gcgcacattt tcagttggaa
    13441 gtagctttgc tgaaagacca tgtgatggta actctggata cgacaggacc aagtttattc
    13501 aaacgtggtt atcgcttaga aaaaggtgga gcaccattga aagaaaacat ggcagcagca
    13561 cttattatgt tgacaaattg gcgaaaagac cgtccatttt atgatcctgt atgtggttca
    13621 ggtacgcttt gtattgaagc agccttaata ggccataata tcgcaccagg attcaatcgt
    13681 gagtttgctt gcgaatcttg ggattggttc agtcaagaaa tcatggaaaa tgttcgatca
    13741 gcagccgaag aaaaagcaga ttatgatatt gaattagata ttactggttc agatatcaat
    13801 ggacggatga ttgaaattgc caaagccaat gcagaagaaa tcggattagg ctcttcgatt
    13861 acctttaagc aacaagcagt caaagacttc aaaacggata aagaatacgg ggtcatcgtg
    13921 gctaatccgc cttacggaga acgtctagga gaagaagaaa ctgttcggaa attatataaa
    13981 gaaatgggcg atgtattccg tccattaaaa acatggagta aatatatctt gactagcgac
    14041 ttagcgtttg aggaatttta cggacaaaaa gcaaccaaaa aaagaaaatt gtacaatggt
    14101 gcattgcgta cggatctatt ccagtattgg ggaactcgtc cgcctagaaa aaaagagaaa
    14161 gacgaataat cttactaatt gattcattca attggatgtt tggaggaata gggaatgaaa
    14221 gaaaaagagt ttttacaaga agtaaaagaa atcaatcttc tacaacaagc tttaggaatc
    14281 ctcgattggg atacacaaac aggtatgcct gaaaaagcta gtgcataccg aagtgaagta
    14341 gacagctatc tttacagcat ttatttttca aaaaaaatcg gtccaaaaat caaagaagcc
    14401 attcgctatt tttctgaaca tccggaagaa ttgtcggaag ttggaactgc cgtttttgcc
    14461 aaagtaaaag aagagtatga tttggaacat cgcgtaccgg aagatcttat gagaaaatat
    14521 acagctgccg tttcttcagc acacggacaa tggcaaaaag ctagagaaac acaacaattt
    14581 tctgattttc aacacgcatt gaatcaaaat attgaattaa caaaacagtt gataccttac
    14641 tggagaaaag atgaaaagac gaattatgat gttttgctga atcaatacga acccgggatg
    14701 actgtcgaaa tccttgataa ggtatttgat cagcttaaag aaggaattct ttcgatccgc
    14761 cgtacgctcc aagaaaaagg gactgaacct gaaacagact ttttaaatcg gagaatgacg
    14821 aaaaaacaac agaagagatt tgtagtgaaa gtaatcgaac aacttggtta tgattttagt
    14881 cgtggtcgtt tagatgacac tgtccaccca tttatgactg gaattaatcc gaatgatgca
    14941 cgaatcacga cacgatggaa cgaaacagat ttcaaaatgg ctgtttttgg agtgatccac
    15001 gaggctggtc atggtatgta tgaacaagac atcgatgata aatatgcgta tacaccggtt
    15061 tatgaaggta catcaatggg tatccatgaa tcacaatcgt tgtttaacga gatcattatt
    15121 ggaagtagtc gcagcttctg gaaaaagcaa tatcctttct ttcaagaatg tgcagaagga
    15181 acattcgatg atattccttt tgacaaattt tatcgttcat tgaaccatac aaaatcaagt
    15241 ttggttcgta tcgaggcaga cagtttgact taccctctcc atatcatcat tcgttatgaa
    15301 atcgaaaaaa tgattttcaa tgaaggggta gatgttgctg aactacctga aatatggaat
    15361 caaaaatatg aagagtatct gggaatacga ccaacaaatg atgtggaagg catcctacaa
    15421 gacgttcatt ggtcaggtgc aagctttggt tattttccct cttatgcatt agggtacatg
    15481 tatgcggcac aattacttca ttctttgcag caagaaatag atgtggaaaa tgttctagcg
    15541 tcagacgatt attctccaat caaaaactgg atgtcagagc atatccatca atatggtgca
    15601 tctagaaaac caaatcaatt aatcatggat gcaacacatg aaccgcttaa tccgcaatac
    15661 ctgatcgact atatgaaaga cctgtatttt aatgtatatc aggtaaaata aaatacaatt
    15721 tacaaagata gctgaaattc atctaatcta tagatgtttt cagctattta cttaagaaag
    15781 aagtggaacg aatggataaa ttacaagtga tcgctgatta tagtatggac caattagcgc
    15841 atgatcaaac aggacatggg agcgatcata ccaaaagagt tgtcaagtta gctgaaaaaa
    15901 ttttggagac ggaaccgcag gcagaccgct ttgtgacgct tgcagcagct tacctacatg
    15961 atacgattga tgataaagtc gttaaaaatg agaacgaggc aaagcaacag cttcgtgtat
    16021 tccttcgctc tttgccaata gtagaggaac agatctcgat gattttttcc attatcgaaa
    16081 atatgtcctt ttctaaaaat ctctctgaag cggccgaact atctctggaa ggtaagatcg
    16141 ttcaagacgc tgatcgtatc gaagcattag gagcaatcgg cattttgcgt actgcttatt
    16201 tcggcggtgg acatgggcac ccgatttttg atcctgaact gtatccgcaa acattcaagg
    16261 acaaagaaaa ttaccgaaaa ggcacgacag tgatcaatca tttttatgaa aaactgttct
    16321 tgctgtctga taaaatgaat actgattacg gatatgaaga agccaaacga agagaaacct
    16381 tcatgcgtga attcttagaa gaattttttc tggaatggtc tattgacgat gaaaatttta
    16441 ctccagacaa ttggatcaaa aataacaaaa aatgatggag gttgtgacag aagtggtcag
    16501 ctccaagata tttcggatca ttttccgtaa aagttacttc tggaacactg tttaccaaga
    16561 ggtcgtgagc ctgccgttca gctccgagtc acaaggaaca atttcaaaaa atagcttctc
    16621 gtgtttgatg aatattgtaa cttgtgattc ggaattggca ggagaacacc gtttattcgg
    16681 aagaatagga ctgtgacaag acttttgtca cagtcctttt ttcgaattca acaagtaaaa
    16741 cttttaagaa atattcagag agtaaaatta aaaattgctg ataaacgaag atggaaattt
    16801 tatgtatttt tattggtcta tgcaataaat aaattaaata ttcagaatac aattgatttt
    16861 aacgaaaggg cttctatcaa tggttgtgtt gtatttatct gcccaggtag agtaaaatag
    16921 atcattatct aaaaggtaat tgttcatact atataagtca tttgaaagta aaaagatact
    16981 gtaaataaaa aatgtggtta aataattctc ataatcagtg taagcgttat caaaaatata
    17041 gcatacttgc tttgtagatg aatgacacga tatatgtttg gtatgttctg caagacagat
    17101 gaatatggag gtggagacaa tgtcttccaa agaactaaca atgtatttag agaaagaatt
    17161 ggatcaactg aaagaaaagg ggttatacaa tacgatcgac atactagaaa gtgaaaatgg
    17221 tgcttgtatc atcgtagacg gaaaaaagat gatcaatcta gcatccaaca attatcttgg
    17281 atttgccaat cgcgaagaat tgaaaaaagc atgtattgaa gcaaccgaaa cttatggtgt
    17341 aggagcagga gcagtccgaa cgatcaatgg ttcgctgaaa atccatcagc aattagaaga
    17401 aaaaatcgca gaattcaaag ggacagaagc agcaatcgcc tttcaatctg gattcaactg
    17461 caatatgggc gctatctctg cagtaatgac aaaagaagat gctatcttat cggatgaatt
    17521 aaaccatgca tctattattg acggttgccg cttatccgga gcaaaaatca tccgaattaa
    17581 acatcaagat atgaaagacc ttgagaaaaa agcaaaggaa gcgatagaaa gtaagaaata
    17641 taaaaaaatc atgtatatca cagatggtgt tttctccatg gatggagaca ttgcacgatt
    17701 gccggaaatt attccgattg ttgaaaaata tggactgatc acctacgtcg atgatgcgca
    17761 cggctcaggc gtaactggaa aaggtgcagg aaccgtcaag cattttggat taagcgacaa
    17821 aatcgatatg caaatgggca cgttatcgaa agctataggt gttgttgggg gttatgtagc
    17881 tggatcgaaa acactaatcg actggttgaa agctagaagt cggccattcc tattttctac
    17941 ttcgctaact ccaggtgcag ctgcagctgc attggcttcg attactttga tgcaagaaca
    18001 tccggaactt gtcgaaaaag tttgggaaaa tgctaattat tttaaagaag aattgaaaaa
    18061 agtaggctat aacatcggta tgtctgaaac acctattact ccagtcattt tgggagacga
    18121 aaaagtgact cagactttct ctaagaagct gatcgaacat gggatttatg ctaaaccaat
    18181 cgtttatcca actgtgcctc ttgggacagg caggatcaga aatatgccga ctgcggaaca
    18241 tacgagggaa atgcttgatg aagcagtagc agtttatcaa aaaataggaa aagaaatgga
    18301 aattatttaa ctaaatttag ttttttcgtc tatttaaaat tagagaacat gttcacgcat
    18361 atattgttaa tcatctgaag atagtagtaa aaaagatcag ggaggatcat catgaaaaaa
    18421 gtattggtga caggatgttt aggacaaatt ggttcagagt tgatcacacg gctacgtcaa
    18481 gaactaggca gtgaaaatgt cattgcgaca gatatacgca tgcctgaagt cattgagaaa
    18541 aacgatatct ttgagatcct agatgtaatg gattacgaaa aaatgcgcag catagcagaa
    18601 caatatcaag tggacacatt gattcatttg gctgctcttt tgtcagctgt tgcagaagca
    18661 aaaccacaat tagcctggaa tttgaacgtg actgggctgg tcaatgcatt ggaagtcgct
    18721 agagaattcc agctgaaatt ttttacgcca agttcaatcg gcgcgtttgg tccaaatact
    18781 ccaaaagatc ataccccaca agatactctt cagcgtccta caaccatgta tggggtaacg
    18841 aaggtagctg gagaactact atgtgattat tattatgtga aatatggcgt agatactcgt
    18901 ggtgtccgtt tccctggatt gatttcttat gcaaccttgc caggaggcgg tacgacagac
    18961 tacgcagtcg atatttatta ttcagcaatc aaagaaggaa agtacactag tttcattgat
    19021 caaggtacat caatggatat gatgtatatg ccagatgcga tcgatgccat tataaaactc
    19081 atgaatgcag atcctgatcg attggttcat cgaaatgcct tcaatatatc ggcgatgtct
    19141 tttgacccgg aagagatcaa agcatctatt caaaaaatca taccagattt tgaaatggat
    19201 tatgatgtgg atcctgtacg tcagtcaata gcagaatctt ggccaaattc actagatgtt
    19261 tcttgtgcgc aagaggaatg gggcttttcg ccgaaatacg acttagatgt gatgacaaag
    19321 gatatgttgg aaaaactgaa agaaaaattg gtagagacgg tataaaacaa gaaccacatc
    19381 caaaaagttt tttatacttt tgggattggc tatggtatga aaggggacaa gggatggaag
    19441 aatatcaagc cacgccagta aaagaagtaa aaatagaaaa tgaattaaaa cgtacgatgg
    19501 gattttttac tgctttatcg actgttatgg gcacagtgat cggtgcaggg gtatttttta
    19561 aagccgcaag cgtagcagaa gttacaggat ccgccagttt gcatatgttt tcttggtttc
    19621 tcggtggtat gatttctgtc tgtgcaggac taacaggagc tgaactggca gctgctattc
    19681 cagaaacagg agggatgatt aaatatatcg aacggattta cggaaataca gcagcttttt
    19741 tattgggctg ggcgcaagtc gtgatttatt ttccagcaaa tgtagcagcc ttgtcgatca
    19801 ttttcggtac acagttcgtc aatctatttg gtctttccca gtcaatgatt gttcccgtag
    19861 ctgttacagc tgctgtatcg atcctgttga tcaatttttt aggttctaaa gctggagggg
    19921 cttttcagtc gattacgctt gtctgcaagc ttattccttt gtttgtgatc gtcattttcg
    19981 gtttgttccg acaagaagga gtagacttcc aactgtttcc gatccaagca ggagaaaatc
    20041 tttcattttt ctcagccttg ggagcgggct tgctagcaac gatgttcgca tacgacggtt
    20101 ggatacacgt aggaaatatt tcgggagaat tgaaaaagcc ggcgaaagat ttgccaaaag
    20161 caatttcttt aggaatcatc ggaatcatga tcgtttattt attagtaaat gcggtgttct
    20221 taagaaccgc atctatcgac ggagtcgcag gaaacagcaa tgcggcaagt gacgttgcaa
    20281 aaatgatttt tggcggcttc ggtggaagac tggtcacagt tggtatcttg atttctgttt
    20341 atggaaccat caatggctat acccttactg gaatgcgtct cccttatgtt atggcaaaag
    20401 aaaacaacct gccttttagc aagctctttg ctaaacttca tgataaaact aaagttcctg
    20461 tagccgcagg tatcttagag ctagtcatcg ctattggcat gatgatgatc ggtggttttg
    20521 atacactaac ggatatgctg atctttgtca tttggatttt ttatacgatg gtctttgttg
    20581 gtgtcattct tcttcgtaaa aaagaacctg atttgtttcg accatacaaa gttccaatgt
    20641 atccgtttat tccactagta gcaatcattg gagggacttt tatcgtatca agcacgctta
    20701 tcactcagac ctttttagct tcgatgggga tagtattgac acttgctggt atcccgattt
    20761 atctctattt aaaacgtaga tagctaaaac attcgaattt tattagaact gcacttctct
    20821 gccaaacaaa atattttatt taaatgagga gtacgtggaa gaaatgtcaa aagtactcgt
    20881 taattttgat cacgtaaatg tcgttttgtc tacgatgaaa tagcaaagta aaagacagaa
    20941 atcgggctca accgatttct gtcttttact ttgatttgcg taactgaact attcggcgta
    21001 cgccgcgata aatatttaat aaaaccaagc aaccagaaat gaaaatggga agccaaatca
    21061 gatagacagg gtagccaagc actttagaca aagcatgtgg agtcatttta tccatttcaa
    21121 gaagtgcttg ctgccaagtc tgaagattga agactagcat gaaacccgaa ataatcagga
    21181 gaataaatcc taaataatga attgtagttc tttttcttac ataagcgatc gtagcataaa
    21241 ggaacagtaa aaaaataggt acgccaaaaa caaagtacat attcagcatc tcgacacccc
    21301 ctttgtgttt atcttacttt actttgaaag aaatgagaaa taatagcctc gttacttgct
    21361 tgaaacagca aaggactgct aagataagag agatacaaag aatcaaggag gagtcatatg
    21421 gcaagttata tcgaaccgat tcgttttgcg ctgattgttt ttccattttt agccttagcg
    21481 atctcatctg ttttttttat ttatgaatat cgaaagtatg gaacatttct agtaacaagg
    21541 gcagttattt tgtattcttt cgttttttat ttattgtgtg cgtatttttt ggtgatctta
    21601 ccacttccgg caaaggagac ggttgcccaa atgacagggc caaagatgga gctgcattta
    21661 tttgcttcat ggcaacattt cagagacaac acggtattgg tcttgtctga acctagtacc
    21721 tacctgccgg caatgaagca gagtgtattt ttggaaccag cttttaatgt tttattactc
    21781 ataccatttg gcatttattt aagatattat ttccggcttt cttttatgaa aacgatgatt
    21841 gctagtttct gcctttctct attttttgaa ttgacgcagc taagtggact ttattttatc
    21901 tacccaagac cataccgctt atttgatgtg aatgatttag tgacaaatac attgggtgga
    21961 atcattggtt ttgtcgttac gccgttattt acatttatgc ttcctacaag aagacgaatg
    22021 gatgaacttt cttatgaaaa aggaaagact gtctcattga ctcgacgctt ggtcgcttat
    22081 ttgatcgact ggacattttt atctcttatt aatttggtag cggcgatttt ctttaaaatt
    22141 gcgttcgatt caggggcagt tacttctcat atctggtggc ttttattaga agtaggggta
    22201 tactttatga ttgtaccgtt tataaccaat ggatggacac ttggcaaaaa aattgtgcgt
    22261 atccgtttgg tggaagaaga ccgcgaacga atttctttaa aagcattgat gatccgctac
    22321 gggtatctgt actttctttt ttatggttta agctggttgg ggcagttgct tcagcaaatc
    22381 atgaattcga caacgaatac ggtgttgcag atgagtgcag tattcatttc tctcggtgtc
    22441 ttgttactac aaggtatttt tattttaaat atcttgctat catttttgag aaaaaaacgg
    22501 attctgtttt atgaaaaagc tagtcacaca tatactgtta gcacgattgg ccaaacaaag
    22561 gatgatgatt ctcgaaatta gctgagcaaa aacgctttgt ctgtgttata atgccaaagt
    22621 cgtgataatt taaagagaag aggtgcacga atgaataatt ttgatatatt ccgcttcaaa
    22681 ccgtttatca atgaagcgtt aaccgcaaag ggcttcaaac agcccacaga ggtccaggaa
    22741 cgtttgattc ctttaattgc aaaaggaaaa aatatcattg ggcaatctca aactggtaca
    22801 ggaaaaacac atacgttttt gttgcctttg tttgaaaaaa tcaatgcaga aaaagaggaa
    22861 gtacaagttg ttattacagc tccaagtagg gaactagctg cacagatcta tgaagcagct
    22921 acacaaatcg ctaaattttc tacaccggag atccgtgtct cgaattttgt cggcggtaca
    22981 gacaaacagc gtcaattaaa tcgtctgcat caccaacagc cacaggttgt agtaggcacg
    23041 cctggtagaa tattagacat gatgaatgaa caagcgctga agattcacac cgcatttgca
    23101 tttgttgtcg atgaagcgga tatgacattg gatatgggtt ttttaaatga agtagaccaa
    23161 attgcaagtc gattaccaga aaagctgcag tttttagttt tttctgccac tattccagaa
    23221 aaacttcgtc cattcttacg aaaatacatg gaaaatccaa tcattgaaga aatcaaacca
    23281 aaagcagtta tttctgaaga cattgaaaat tggctgattt caacaaaagg taaagatgag
    23341 aatcaaatga tttatcaact gctgacaatt ggtcatcctt atttagcaat cgtatttgcc
    23401 aatacaaaac agcatgtgga tgaaattgcc gattacttga aaaaccaagg cttgaaagta
    23461 gcaaagattc atggagacat caccccaaga gagagaaaac gagtgatgcg acaagtacag
    23521 aatctggagt atcaatatgt tgtagcgact gatttagctg ctagagggat cgatatcgaa
    23581 ggtgtctctc atgttattaa tgcagaaatt ccagaagatc tagacttctt tatccatcgt
    23641 gtcggacgta ctggacgtaa tggcttaaaa ggaacggcca ttacgttata ttcgccaaat
    23701 gatgagcaag ccattgatgc gatagaaaaa ttgggcgtct catttgaacc aaaagaaatc
    23761 aaaaacgggg aaatcgtcaa aacatacgat cgtaatagac gtgccaaacg agaaaaatca
    23821 aaagatgtat tagatcccac gctgattggt cttgtgaaga agaaaaagaa aaagatcaag
    23881 cctggttata agaaaaaaat cgattgggca attgcagaaa aaaataaaaa agaacgcaaa
    23941 atcgaacgca gacagcaaag ccgtactgct cgtaaaaata agaaaaattc acataaataa
    24001 aaaacaagag gttgtgacaa aaggcgtgtc acaatctctt gtttttaagc tagacttttt
    24061 ttcattccga tacaaagtac ttcatcttca ataaaaggtt ctgtgcaagt atgataccct
    24121 tgtttttcat aaaaatctac agccgtcatt tcagctacta cataactagt ctgacaacct
    24181 tctgaaaccg cccgtttttc agcagcttca agcaactgac cgcctaaccc ttttcctcgg
    24241 tattcttttg ctacgcaaaa tcgttccggc ttgaagcaag taggtgtttt cttttcataa
    24301 cgcaaagtag ctactggtct tttttgatca agaaatagat aataggtttg ttgcgggata
    24361 tcttttccgt cgaattctag ttctgcagga atcccttgtt ctaatacaaa aacttgatat
    24421 ctcaaaaagc agctagctgc gcgtatccat tcatccgctc caactatttt tttcatcata
    24481 ttcctccttg tcatgaatag cagagatgta ttgattcctt tattcttatc gtaccaaaaa
    24541 tcgtggagta tgaccaaaag gaaatgaaaa taaaaattga gatattgaca gtgatcaata
    24601 gaatgcgtat actaattaag tacgaaaagg atatgatttg ttttttaaga ctcacagaaa
    24661 attctttctt ggctgaaaaa agaatagtca ctaatcaaat tgctcccttt ttctattttg
    24721 gtagaaatac cgacgcagct cagcgttatc gagtttaaga ggtaatgatt acacatcatt
    24781 gcaatcaagg tggtaccgcg agttttcgtc cttggctgtg atggtgtttt tttgtattta
    24841 atttatagaa agcaggtaat gacatgaaac aattaagtag tgcacaagtt cgacaaatgt
    24901 ttcttgattt cttccaatct aaaggacata cgattgaacc tagtgcgtct ttgatccccg
    24961 tgaacgatcc aaccttattg tggatcaact ctggtgttgc aacattgaaa aaatattttg
    25021 atggttcagt tgtgccagaa aatccaagaa tcacaaatgc ccaaaaatct attcgaacaa
    25081 atgatatcga aaatgtcggt aaaacagcac ggcaccatac gatgtttgag atgctgggta
    25141 atttttcgat tggtgactat ttcaaaaatg aagccatcca ctgggcatgg gaatttttga
    25201 ctgatccaaa atggatggca ttcgatccag aaaaacttta tgtaacagtt tatccaaaag
    25261 atacagaagc aaaacgcatc tggcatgaag aagttggcct gccactagat cacattattg
    25321 atgtagaaga caacttctgg gatatcggtg caggcccatg tggtccggat acggaaattt
    25381 tctatgaccg tggagaagct tataacgatt tagcagaaga cgatcctgaa aactatccag
    25441 gaggagaaaa tgaacgttat ttagaaatct ggaacttagt attttcagaa ttcaaccata
    25501 agcctgataa cacatttgaa ccacttcctc ataaaaacat tgatacgggg atgggattgg
    25561 aacgcatggt ctcaatcgtt caagatgcgt caacgaactt tgagacagac ttgtttatgc
    25621 ctattatcca cgccgtagaa aagctaagca aacaagtaaa atatggtgaa aacccagtaa
    25681 cagatatttc ttacaaagta atcgcagatc atattcgcgc cctttcattt gctattggcg
    25741 acggtgcatt gccatcgaat gaagggagag ggtatgtctt acgccgtttg cttcgtcgtg
    25801 ctgtcatgca tgggaaaaaa ttaggtattg aacaagcatt cttatatcgt ttagtccctg
    25861 ttgttgggga aatcatggta agttattatc ctgaagtact tcaacaaaaa gaatttattg
    25921 aaaaagttat ccgtacagaa gaagaacgtt tcaatgaaac aatcaatgaa ggtttggaaa
    25981 tcttaaatca agtgatccaa gaagtaaaaa acgaaaacgg gacgactcta gcaggaaaag
    26041 acatcttcaa actatacgac acatatggct tcccggtaga attaacggaa gaggtggcag
    26101 aagatgaagg actgaaagtt gatcatgctg gttttgaaaa agaaatggat gcacaacgcg
    26161 aacgtgctcg ctctgcccgc agtactgaaa aatcaatggg ggtacaatct gctttattga
    26221 cagatatcaa agtagaaagt aaattcattg gatacacaga acaagaatca gatagtcaat
    26281 tattggtcat cgttaaaggt gagtctcttt tgaatgaagt cgcagaaggt gaagctcaaa
    26341 tgatctttga tcaaacacct ttctatgcag aaatgggtgg gcaagtagcg gatcaaggat
    26401 ggatcatgaa tgaagtaggc gaaatcgtag cagttgttac agatgttcaa aaagcgccta
    26461 atggtcaatt tatgcacacc gtagaagtaa aagcaccaat gagtgaagga caaacctatc
    26521 atctattgat cgatcaatcg atgcgcaatc ggatcatcaa aaaccataca gcaacccatc
    26581 tactgcacaa ggcattgaaa gaagtacttg gcgagcatgc aaatcaagct ggttctttag
    26641 tcgctcctgg acatttacga ttcgacttta cccactttgg tcaaatcact tcagatgaat
    26701 tagctgaaat ggaaagaatc gtcaacgaaa aaatctggga agctatccca gttgaaacaa
    26761 ttgaaacaga tatcgataca gccaaaaaca tgggtgcaat ggctttgttt ggtgaaaaat
    26821 acggacatga cgttcgagta gtaaatattg gcggctggtc aatcgaactg tgcggaggca
    26881 cacatgtagc aaatacagaa aacatcggaa tcttcaaaat cgtttctgaa tcaggaattg
    26941 gtgcaggcgt gcgacgaatc gaggctgtaa caagtaaaga agcgtacgaa ctacttgaag
    27001 aagaagaaaa acaactaaaa caagtagcaa caattgttaa atcacctcaa ttgaaagaag
    27061 ttgtttcaaa agcggaacaa ttgcaacagc aagtaagaga gctacaaaag gaaaatgaac
    27121 aattagctag caagttagcg aatcaacaag ctggtgatgt attcaaagat ccagaaacga
    27181 tcaatggaaa aacaatcatt gcagcacaag taaaagtgaa agatatgaat cagctgcgtc
    27241 aattagctga ccaatggaaa caaaaaaata tttcagatat tcttgtttta gcaaacgaac
    27301 aagaaggaaa agtcagtctg ctagcggcaa tgacgaaaga atcaaatgaa gctggactaa
    27361 aagctggcga cttgattaaa gcaatcgcac cgaaagtagg cggtggcggt ggtggacgtc
    27421 cagacatggc acaagctggc gggaaaaatc ctgctggtat tccagatgca cttaaagctg
    27481 cgaaagaatg ggttgcgcaa caatagggcg tttacgtttg acagaaaaac aaaagttcaa
    27541 acattgaacg aaacatgatt atgggggcga agacaaacaa gtcttgccct catacttttt
    27601 tcaattggat ggcgcatcta atcaaatggt cggattcgga tggtctttag ttgacgaaag
    27661 tgacagaaat ttttacgatt agtataaata aaaaggggtg aaaaaatgaa tagtagaaat
    27721 aagacctttc gactagtctt acgtgcgatc ttgttagcaa tcatcatcgt acaagcgatg
    27781 gttccttggc tggggtttat tccattagga ttcattagtt taacaattat ccatatcaca
    27841 gtaattattg ctgcagtagt attaggccca aaagatggca tggtgattgg cttgttttgg
    27901 ggaatcgcta caattgttcg tgcctatgcc atgccaacaa cgccatttga tacattagtg
    27961 ttcacgaatc cgattatctc ggtagttccc agagtacttg ttggcttagt agcaggcttg
    28021 gttttccact ggatttatca aaaaaataaa tcaataacga tcagctcagc ctttgcaggg
    28081 gtactgggtt cattggtcaa tacagtctta gtgttaggat tcatgggatt attttataca
    28141 ggtgcaacgg cagatgccta tggtgtagac tctgctcttt tatttaaaac cttagcaggg
    28201 gttgcagcaa tcaatgggat accagaagca ataggtgcgg gaatcataac accattacta
    28261 gcaaaagcgt tatttgcagc aacaccgttg aaaccagaat aatgaccatc ttttaatagt
    28321 aataggacgc acgatagata gaaaaaggaa gagaggagag gaaaacaatc gtcctctcca
    28381 tctttttacc tgagcaacaa gcaaactaga aggggtaaaa aacttggcta agaaacaaga
    28441 agaaaaaaag aaacaaatac cgcaacagcg gacaaaaaaa ggagtactag cgggtatctt
    28501 aggattggtt acgaatctga tcttgtttgt ctctaagcta atgatcgggc ttttttcagg
    28561 aagtgtctcc attatggctg atgcagtgaa cagtctatca gatactgctt catccatatt
    28621 gactttgatc ggatttaaaa ttgctgcaaa gccagcagat caggaacatc cttatggaca
    28681 cgaacgcttc gaatatatca gtgggttatt tgtttcgatc atcattactt atgtaggatt
    28741 tcaattttta gagtcttctg ctgaaaaaat catccatcca gaaaatgtct ctttaacacc
    28801 catcgtcttt tttgtcctaa tcatttccat ctttttgaaa tttgcacaag gcaaaatgta
    28861 tcaggttatt gcgcaatcca ttgattctga aacattgaga gctacagcaa cagatagtta
    28921 taatgatgtc tttactacac tggctgtatt gctttcagca gcaatcgaat gggtaactgg
    28981 atggcgtatt gatggatata taggttttgc tttagcatta tatatccttt atagtggatt
    29041 gacgatgctt cgaaattttg tatacgaact attaggaagt cgcccaacgc cagcagagat
    29101 tgaagaaatg gaagagcatt taaatgcttg cgatgatata cttggctatc atgatttatt
    29161 ggtgcataac tatggaccca ataaacgctt tgcatcagtc cacatcgaag tagatgaatc
    29221 aatgaatttg aatgtggctc ataagattat tgatctgata gaaaaagact ttaaaaagtc
    29281 tttggatgtg gaacttgtct gtcacttgga tccaatgcct gttgataacg aaaaatacta
    29341 taatatcttg cggcagttaa agcaaattct tcttgaaaca ggtacagggc ttaatattca
    29401 tgattttaga gtaattcatc aaggaaaaac gctgcaattt gatgtagtgg tccctgagaa
    29461 attccagttt gttgatacag aactagaaag aatcttacgt agtcaaatag aagaaaaaat
    29521 cggagattac caattggaaa ttacgtttga tcacaattat cttttatatt aaatgagaaa
    29581 ctataaaaag actccttaaa gtttttaatt taaagagtct ttttttcttt taattgttta
    29641 tggagagcct gacgtgcaag attatctgct cctttatttt tgttttcggg caaccattgc
    29701 aggatcaata aagaaaattc ggatagaagt tcttggattc tttgaaggta gggaaggaag
    29761 agctcgttct tcgttgcgtt tttgtcgacc gtagaaacta gtgttttgct gtctgaaaaa
    29821 cagaaaatcg tttcattctg taagtcgttt ttctttagaa attctaaagt ttttaaaaag
    29881 acttcgaact ctgcttgatg gtttgtccct atagaaagcg ggatagatag ctgttcttgt
    29941 ttaccttgat agacaatcaa gattccacct ccactaggac cagggttgcc ttttgtagag
    30001 gcatcaatgt atacttttaa cataaaaaga gattctcctt tttgttcaaa ctaatgttat
    30061 ggtaagatta tatcattact gaatcggtga gaggttagag aatgaaaaaa aatattcgat
    30121 ggcagcctga actggcacat acgatcatct attggtcatt gaccttgatc attctgtttt
    30181 ttagtctgat tctttcttta gaaaacacag gtccttattg gaaaagcaat tttgttttag
    30241 gtatttttct tgtttttcta tttctaggtt tgcggcgttc ttttatttta aaatcaaact
    30301 ctgtgattat acgttatgct gctttttgga aagaccgaga aatatctgtc ggacagattc
    30361 gatctttgac gccaactaat actggggtgc aaatgatgtt gcaagatgag aaagccccat
    30421 ctgttttcat gatgcgaaaa aaagatcaag tagtcctttt tgatcatttt gaaaaaaacg
    30481 gacccaaatc agaatcaatt ccttatcctt atacgaaaaa aagaaaaagc taattttaaa
    30541 aatcagaacc taaaaaaacg aatttttaca ggaaaagaaa agagtgagac gtacggttca
    30601 tcccgtttag cgtctcactc tatttgttta tctattcatg catactagta tcggtagtct
    30661 gaacgttgcc tttcttgaca tgggcagctt gtgttcctcg attcccttcg atgatctcaa
    30721 attcgaccac ttgattttct tccaaagttt tgaatccctc ttcttcaatc gcagtaaaat
    30781 gtacaaaaat ttcttctgtg tcatcataac tgataaagcc gtatcctttt ttgttgtcaa
    30841 accattttac aattcctgtg gtcaaagcac tcacgacctt tctaaagtct gatcatagcc
    30901 ttcattataa agagtattta taagtacgtc aaataaatta aaaacctgta attttatgtt
    30961 ttttttctga ataaaaaaag gtataattgg catatcttaa caaagaagat attttgttga
    31021 ataaagttcg tgaaaagggg taatcaaatg aagaatgatc tgcaaatttc acaagaaaca
    31081 gagctagaac cgattgttga agtggctagt aaattagatc ttaaggaaga cgatttggaa
    31141 ctgtatggga aatataaggc gaaaatcaat ttttctggaa tcaatcgatc aaaggataac
    31201 gctgaggggc atttgatctt agttacttcc atcaatccaa caccagctgg cgaaggaaag
    31261 tcaacgatca ctgtagggtt aggagacgcg ctcaataaaa tcaataaaaa atctgttatt
    31321 gcattgcgag aaccctcact tggtcctgtg atgggaatca aaggtggtgc tgcaggcgga
    31381 ggatatgcac aggtattgcc tatggaagat atcaacttgc attttactgg tgacatgcat
    31441 gctatcacaa cagctaataa tgcattatct gctcttttag ataatcatat tcatcaagga
    31501 aatgaattga acatcgatgc ccgccgagtg atttggaaac gggtagtcga tctcaatgat
    31561 cgtgaactgc gtaaagtaat tgttggcttg ggtgggccaa tccaaggtgt tccaagagaa
    31621 gatgggtttg atatcacagt tgccagcgaa atcatggcta tcttgtgtct tgcggcagac
    31681 cttgaagatt taaaggctcg tcttgcaagg atcgttgtcg gctatacatt tgaccgtcag
    31741 cctgtgactg ctggagactt gaaagcagaa ggcgctctgg cattactgct gaaagatgca
    31801 atcaagccga atcttgtaca aacaatttac ggaacaccag cttttgtcca cgggggaccg
    31861 tttgccaata tcgctcatgg ctgcaatagt attttagcta ccaagacagc attgagatta
    31921 gcagattacg ttgtcacaga ggcaggattt ggtgcagatc ttggcggaga gaaattttta
    31981 gatatcaaag tacctaactt gaaaaaagcg ccggatgctg tggtgattgt cgccactgtc
    32041 cgtgcattga aaatgcatgg cggaatgaaa aaagatgaac ttaaaaatga gaatcttgat
    32101 gctttgaaag taggttttgc aaatttaaag cgccatatac gtaatatgga acaatatcag
    32161 ctacctgtga tcgtcgcaat caatgaattc gtaacagata cagacaatga actcacactg
    32221 ttggagcact tgtgtgaaaa tcaggggatt cttgccaaga gagcgagtgt atgggcagat
    32281 ggagcagaag gcggcgttga tttagcaaaa gctgtagtac gattgattga tagaaaagaa
    32341 gctaattaca agccattata ccgcctagaa gaaacgatac aagaaaagac agaaacaatc
    32401 gtcaagaaaa tttacggagg aaatggtgtt gttttcagca aaaaagcgca aaaacaaata
    32461 gaagagttta cgaagaatgg atgggacaaa ctgccgattt gtatggcaaa aacacagtat
    32521 tcattttctg atgatccttc actgctaggt gcaccggaag atttcaccat cacgatccgc
    32581 gaaatcatcc caaaattagg ggcaggattt ttagtagcct tgactggaga tgttatgacg
    32641 atgcctggtt tgcctaaaaa acctgctgca ttgaatatgg acgtgacaaa tgacggagaa
    32701 gtgttgggat tgttttaagc ttgattataa tctaccatcg aaggagctgt attttgacta
    32761 cagcttcttt ttttagacga tagggaatta attttgttta cactaggatc attctttttc
    32821 tttattgtat tttgagctat actaaagcat aataggaggg tatgatgaaa acgacattta
    32881 taagaaaaac cggtttttac ggtatgggta gctcgattca gctaagaatc aacaatgaaa
    32941 aacgcagtgt tctcagtcat gaacaaaaga taacgcttga tctggaagtg ccctttacga
    33001 tgcaggtcac cttttattgg ctaaaaagcc ccgtttatac agtgaaagaa gcaaagcctg
    33061 cttatgtgat cacaatgaat tacttgattc ttcaaatcta tccgttttta tttctagcaa
    33121 ctgggttggg ggccgttttc ctacaaaatt tatttttcag tttttttgca gttattgtgt
    33181 tgattgcgtt ctttttctat ataaaaaacc gggcatattt gatcaaggag gaaaattatg
    33241 gcaaactctg atcttttttt aatcagtttc aatcgaatcg aaaaatggat gagagacgag
    33301 atgggaaatg cacgaaatat gggatttacc gaacttgtca gacgcctagc tcaaagaaaa
    33361 caattaatga tcagaaaata cgaagatgat cttttacagc ttgctcagtt aaggaatgcg
    33421 atcgtccatg atcggatcgc agtcgatttt gtcattgcag agccaaatga ttggacagtc
    33481 aagcgaatcc agctgatcga acaagaactg attcagcctg aaaccgtgct gcctcgattt
    33541 gcaaaacgtg taacaggttt tgaacaagat cttccgttac ttgaactgtt aaagatagta
    33601 gcagaaaaaa ggtattcaca atttccgtta tacaataaag gaaaatttgt agcattgatt
    33661 actttacgta atatcgggtt ttggctagca aaagaaagcc aaaaaggtcc ggttgattta
    33721 acaaataaac gagcgttgga tttgatcatc caaaacggaa agtacacaaa ttatcatttt
    33781 gttccagcta aaacacatat ttacgaggtt gaagcgatgt ttcgtgagca agggactttg
    33841 gaagccgtac ttatcactaa agatgggaat cccgatggta atctgctcgg gatcgtacga
    33901 cctcgtgata tttataaaca aatagaaaag gattgatttt ttgttaatta tttattggat
    33961 attcagcgct ttgctagtag gagtggatca gttggtcaaa tggtggatca ctgataatct
    34021 agcgcttgga gagacgaaaa atcttatacc aggtattttg tcactgaatc atattcgaaa
    34081 cactggagcc gcatggagta tgctagaagg gaagatgtgg ttctttacta tagtgacact
    34141 cattgcagtc gttgtgattc tcacattgat gattaaaaat cgtgaaaaag gtaatcgctg
    34201 gttcatgatt ggattgagtt tgattctagc tggtgcgatt ggcaatttta tcgaccgtat
    34261 tcgtcttggc tatgtagtag atatgttcca gacagatttt atgagctttc cgatttttaa
    34321 cgtagcagat gtgacacttg tatgtggagt gatttgtatg ttaatttata ttattttaga
    34381 tgaaaaggat caaagaaaaa aatgacagaa cttaaaacga cgattcacca tgaatctggt
    34441 aggatcgata aagtattgac taatttattc accgattaca gtcgttcgca aattcagctg
    34501 tggctgaaaa atggagcggt ttctgtcaat ggacagccag tcaaagcaaa ttacaaagta
    34561 aaacaaggag acgagattac tgtcttgatc cccgaaccag aaacattaga tgtggtccca
    34621 gaaaatatcc cgttgacgat cgtttacgaa gatgaagatg tagcggtgat aaacaaacct
    34681 caaggaatgg tcgttcatcc ttcagcaggt catacgcatg gcacaatggt caatgcgctg
    34741 atgtaccata tcaaggatct ttctacgatc aacggcgtta ttcgtccagg tattgttcac
    34801 cgaatcgaca aagatacttc cggactgcta atggttgcca aaaatgacca ggcacacgaa
    34861 tcattagctc agcaattaaa agataagacc tctttgcgca aatatgttgc tttagtccat
    34921 ggagagattc ctcacgaaaa aggaaccatc aatgcgccaa tcggccgttc gaaggaaaac
    34981 cggaaaatgc aggcgataag agaagatggg aagtcggctg tcacacattt tactgtattg
    35041 gaacgtttcg aaggatttac tctggttgaa ttaaaactag aaaccggaag aacacaccag
    35101 atccgtgtgc atatgaagta tatcggttat ccgttagctg gagatccagt atacggaccg
    35161 aagaaaactt tgaaaggcaa tggtcagttt ctccatgcgc aaatgttagg ttttacgcac
    35221 ccgactacaa acgaaaagat gatctttgaa gcacctcttc cagaagtttt tgaaaaaact
    35281 ttagaaaaat taagaaaaaa cattgcattt taatttagat aacggtatag taattaacaa
    35341 cgaaagcata aaacttaata gaattccttt aagtttagtc ccgtgaggct aagaagggtg
    35401 ggagcaagga tacgtatggt gcgccataag tagattgctc attgacatag tatagctatc
    35461 agtgtatcag tatacgctca gtctaaacat tcctcctgtc ctcttagggc aggagttttt
    35521 ttatgcaaaa aagtaggggg aatgaaaatg caagcaaaag aagtagtaga tcaggtaacc
    35581 atgaagcgag cattgactcg aattacttat gagattatcg agagaaacca tagtatccaa
    35641 gatatcgtat tggtaggtat caaaacacga ggaatctaca ttgcatcaag aattgccgaa
    35701 cgcctgaagc agctagaaga tatcgatatt cctgtaggag aactggatat cactttatac
    35761 cgagatgaca aaaaagaaaa tccagaagaa ccggaacttc attcttctga catcccagtt
    35821 tccttagaag gaaaagaagt tattttgatt gatgatgttt tgtatacagg acgaacgatt
    35881 cgagcagcaa tggacgcagt aatggatttt ggcagaccaa gaaaaatttc attagcagtc
    35941 ctggtcgatc gtgggcatcg agaattgcca atccgtgcag attatgttgg gaagaacatt
    36001 ccaacatctc gggcggaaga gattttagta gagatgcaag agcttgatgg acaagatcgc
    36061 atcatgattt tgaaagagga agattagagg agagagaaac atgtcggaga aaaaaggatt
    36121 tcataatgat gacgtggtgt tagatatcca cgatcgccca accccaggac attgggtagg
    36181 attaagttta caacacttgt tcacgatgtt cggagcaacc gtgttagtcc ccatcttagt
    36241 aggtatcgat ccaggtattg cgctagtcag ttcaggacta ggtacattgg tgtacttatt
    36301 tactactaaa ggaaagatac ctgcgtactt aggaagcagc ttcgctttca ttgcagcgat
    36361 gcagcttttg atgaaaacag atggttatcc agcgattgca caaggagcag tcacaactgg
    36421 attagtctat ttgattgttg cgctgatcat caaaaagatc ggttcagcat ggctggataa
    36481 aatcttgcct ccaatcgtcg tcggtccagt catcatggtt atcgggttgg gacttgcggc
    36541 aaatgcagca acaaatgcta tgtataatca gggcacgtat gatttcaagt atattgctgt
    36601 agcgctgatc acattaggat taacaatttt cttcaacatg tggctaagag gttttctcgg
    36661 attgattccg attttactag gtattattag cggatacatc gtagccttgc tgtttggaat
    36721 cgtggatact aagccaatcg tagacgctgc ctggtttgct gttcctaact ttgagattcc
    36781 ctttgtacaa tatgaaccaa agctatatct tggagcaatc acaacaatgg cacccatcgc
    36841 ttttgtgacg atgacagagc atatcggcca tcttatggta ttgaataaac taactaaacg
    36901 taatttcttt gaagaaccgg gtcttcataa gacgttgaca ggtgatggat tagcacaaat
    36961 cgtggcagcc tttgtcggag gtcctcctgt aacaagttac ggtgaaaaca ttggtgttct
    37021 ggctatcaca cgtgtccata gcgtgtttgt gatcggtgga gctgcagtat ttgcagtcat
    37081 tttaggattc gtaggaaaat taagcgcttt gattttaagt atccctggac cagtcatctc
    37141 aggaatcagt tttatcttat ttggagtaat tgccgcaagt ggaatgaaga ttttaatcga
    37201 gaacaaaatc gattttgata aaaagaaaaa tctgttgatt gcttcagtta tcttagtagt
    37261 aggaattggc ggactcgtct ttgaagtagg cacatttaca ttgtcggcaa tggcattagc
    37321 aactgtactt ggcatcattt taaatcttat cttaccagaa acttcacgaa gcgaagaaaa
    37381 ctaaggagga aacattatga tcatcaaatc agaacgtatc agcttaaagc atttattgac
    37441 cgttgaggca cttactgatc aagaagttat gggcttgatc cgcagagggc aggaattcaa
    37501 gcaaggtgct aattggactc cgcaaaagcg ccagtacttt gctacaaacc tctttttcga
    37561 aaatagtact cgcacacata aaagcttcga tgtagcggag aaaaaactag ggctagaagt
    37621 gattgaattt gaagcaagca caagctctgt ccaaaaaggg gaaacactat acgatacagt
    37681 actgacgatg tcagcattag gcgtagacgt agcggttatc cgtcatggtg atgagaacta
    37741 ttatgacgaa ctgatacaaa gtaaaacaat ccagtgttcg attatcaatg gaggagacgg
    37801 aagcggacag catccaaccc aatgcttgct tgatttgatg accatttatg aagaatttgg
    37861 tcattttgaa ggtctgaaag ttgccatcgt aggtgatatc actcactctc gcgtagctaa
    37921 atccaatatg cagatgttaa aacgtctagg cgcacaagta ttcttctcag gtcctagaga
    37981 atggtacgac gatgaattcg aagtgtacgg acattatatg ccattggatg aactactaga
    38041 tcaagttgat gtcatgatga tgcttcgggt gcaacacgaa cgccatgatg gaaaagaaag
    38101 tttctcaaaa gaaggctacc atcaggaaca tgggttaaca gttgagcgtg cgaagaagtt
    38161 gcaaaaacat gcaatcatta tgcacccagc accagttaac cgtgatgtag aattagctga
    38221 ttcattagtg gaaggattac agtcgcggat cgttcagcaa atgagcaatg gagtattcgt
    38281 cagaatggca atcttagaag cagtgctaaa tggaaaagcg taattatagg aggatgaaaa
    38341 aatgaaaaca ctcattaaaa acggtcagat caatacacga aaaaatatga cgacaccagc
    38401 agaaatttgg atagaagatg ggcgaatcaa agcaatcggt acaggattct cagaagccga
    38461 atttgatgaa gtgtttgatg caaagggaca attgatcaca cctggtttag tggacgtcca
    38521 tgttcatttg agagagccag gatttacata caaggagacg atcgaagctg ggactcgctc
    38581 agctgctcgt ggcggtttca ctactgtatg cgcgatgccg aatttaaatc ctgtacctga
    38641 tacggcagaa aagctgagac aagtatatga catcattaga aaagatgcag ttgtgaaggt
    38701 attgcagtat gcgccaatca cagaaaatct gcgaagtgag aaattagttg atcaagaagc
    38761 actgattgaa gaaggtgctt ttgctttcac aaatgacggt gtaggtgtcc aaacagctgg
    38821 gacgatgtat ctggctatga aagaagctgc aaaaaacaat aaagccctag tagcccatac
    38881 agaagatgaa tctttattat ttggcggcgt tatgcacgct ggcaagaagg cagaagaact
    38941 tggtcttccc gggatcttaa gcgtaaccga gtcttctcaa attgctagag atctgttgct
    39001 agctgaagca actggtgttc attaccatgt ttgccatgtt tctactaaag aaagtgtccg
    39061 tgtaatcaga gacgcaaaaa aagcaggaat ccacgtaaca gcagaagttt caccccatca
    39121 tcttattttg atcgacgaag atattcccga agatttcggc ttttggaaaa tgaacccgcc
    39181 gctaagagga cgggaggaca gagaagcttt gatcgaaggc ttgcttgacg gtacgatcga
    39241 ttgcatcgct acagaccatg ccccccatgg attagaagaa aaaagccaaa gtttcatgaa
    39301 atcaccattt ggtatcgtag gaagcgaaac agcgtttcaa ctgatctata ctcattttgt
    39361 agaaactggt cgattcacgt tggaacaagt gatcaattgg ttggcagtta aaccggctga
    39421 gatttttggt ttgaatgcag gaacgctaac ggttggtgcc cccgctgatg tagcagtttt
    39481 tgatattaca caaacttgca cgatcgataa agaagacttt ttatcaaaag gggagaatac
    39541 accttttatc ggctggaaag taaaaggcga aacacaaatg acttttgtca acggaaaact
    39601 tgtgtggcag aagggagaat aaaagtatgg aacgtttgct tatattagaa gatggaacag
    39661 ttttcgaagg aaaggctttt ggtgcagatg ctagtgtctt cggagaaatc gtttttacaa
    39721 caagtatgac tggttatcaa gaaactatca ctgatcaaag ttttaacggt caaatcatta
    39781 catttaccta tcctatggtg ggtaattatg gggtaaatag agatgattat gaatcgattt
    39841 ctccaacatg caaaggagta gtagtaaaag aacatgcacg tgtagcttca aactggcgaa
    39901 accaaatgac gctagattca tttttgaaac aaaaaggaat tcctggaata tcaggtatcg
    39961 atacgcgcgc ccttactcgc aaaatccgtg aagtcggaac gatgaaagct agtatagttg
    40021 atgctggaga ttccttcgaa catgcatttg atcagctgaa agcaaccgtt ctagcaacca
    40081 atcaagtgca gcaagtctca actaacaaac cttatccaag tccaggaaca ggtcggaaag
    40141 tcattgtcat tgacttcggc ttgaaacata gtattttacg agaattgagt aaaagaaact
    40201 gtaacctcac agtacttccc tataacacag atgccgagac gattttagcg ctatcaccag
    40261 acggagtcat gctgacgaat ggacctggtg atccaaaaga tgtgccagaa gcaatcgaaa
    40321 tgatcaaaaa aatccaagga aaagtaccga tttttggtat ttgtctcggt caccagttat
    40381 ttgctttagc caacggtgca gatacctata aaatgaaatt cggacatcga ggattaaatc
    40441 acccggtacg agaagtagcg accggaagaa ttgattttac ttctcaaaat catggctatg
    40501 cagtcgatga agccacgatt gatccagaaa aactcatcgt aactcatgta gaagtaaatg
    40561 acggaacagt agaaggagtc aaacacagag attatcccgc tttcagtgtg cagtttcatc
    40621 cagatgctgc ccctggtccg catgatgcac tacatttatt tgatgaattt atggaaatga
    40681 tggacgcagg aaaggagcag taaacagatg ccgaaacgta cagatatcaa gaaaattatg
    40741 gtgattggat ctggtccgat catcattggt caagccgcag aatttgacta cgcaggaaca
    40801 caagcctgcc ttgccttaaa agaagaagga tatgaagtgg ttttagtaaa ttctaatcct
    40861 gcgacgatca tgacggataa agaagtggca gatcaagtat atatcgaacc gatcacactt
    40921 gaatttgtat caaggattct tcgaaaagag cgtccagatg ctctccttcc aactttaggc
    40981 ggacagacag gcctcaatat ggcaatggag ctggcagcat caggtatttt agaagaattg
    41041 aacatagaac ttctaggaac aaagctttct gcgatcgatc aagcagaaga tcgtgatcta
    41101 ttcaaacagt tgatgaaaga attgaatcag ccaatccccg agtctgaaat cgtcaataca
    41161 gtagaagaag ctgtagattt tgcaaataaa atcggctatc ctgtcatcgt tcgtccagcc
    41221 tttactttag gcggaactgg tggaggtatg tgtgaaaatg aagaagaatt acgtatcgta
    41281 tcagaaaatg gattaagtct ttcccccgct acccaatgtt tgattgaacg aagtattgca
    41341 ggttttaaag aaattgaata tgaagtgatg agagattcgg ctgataatgc aatcgttgtc
    41401 tgcaatatgg aaaattttga cccggtgggt atccatacag gtgattctat cgtatttgcc
    41461 cctagtcaaa cattgtctga ccatgaatat caaatgcttc gtgatgcttc tctttcgatt
    41521 attcgagcac ttaagatcga gggaggctgt aatgtccaac tcgctcttga tccacatagt
    41581 tttaattatt atgtgattga agtcaatccg cgtgtttctc gttcatctgc gttagcaagt
    41641 aaggcgactg gctatccgat cgctaagcta gctgctaaaa tagcgcttgg attaacatta
    41701 gacgaaatga aaaatcctgt tacaggaacg acttttgcag aatttgaacc tgcattagat
    41761 tatgttgtag ctaaaattcc tcgctggccg tttgataagt ttgaaaacgg agaacggctt
    41821 ttaggcacgc agatgaaagc aactggtgaa gtgatggcta tcggtcggaa tatcgaagag
    41881 tctttattga aagctgtgcg ttctttagaa atcggtacac accatatcga acttcctgag
    41941 ttggcagaca tcacggacga tgacctaatc caaaaagtgg ttcgagcaca agatgatcgc
    42001 ctgttttatg tagctgaagc tattagaaga gggtacacca tagaagaatt agcggatttg
    42061 acaaaaatcg atttattctt tttagataaa ctgttgcata tcgtagaaat cgaacagcag
    42121 ttgaaacagc agccgttgga tgacaagata ttgaaaacag cgaaacaaaa cggttttaca
    42181 gacaaaaaag tggcggagct gtgggaaata aacgaagaag ccgttcgcca gatgagaaaa
    42241 gaaaatgatt tgattcctgt atataagatg gttgacactt gtgcagcgga attcgaatca
    42301 aaaacacctt atttttatag ttcctatgaa tatgaaaatg aaagttcacg atcagaaaag
    42361 ccctcaattc tagtactggg atctggtcct atccgtatcg gacaaggggt tgaatttgac
    42421 tatgcgactg tgcattctgt caaagcaatc cagcaggcag gatacgaagc aatcatcatg
    42481 aacagcaatc ctgaaacagt ttcgactgac ttttctattt cggataaatt atattttgag
    42541 ccattgacct tcgaagatgt gatgaatgtg atcgagctag aacagccgat aggagtaatc
    42601 gttcaatttg gtggtcagac agcaatcaac ttagccgaac ctttgactgc tgctggtgta
    42661 aaaatcttag ggacaacgat cgaagacttg gatcgtgcgg aaaatcgtga tttattcgag
    42721 caagcgctga aagagttgga tattcctcaa cctttagggg atactgctac aagtaaagaa
    42781 gaagccgtgg ttatcgcttc gagaattggt tatccagttc ttgttcgtcc aagctatgta
    42841 ttaggcggac gtgcgatgga aatcgttgaa aaccagcgag atttagaaga ttacatggaa
    42901 catgctgtga aagcatctcc agaacatcca gtactagtgg accgttactt gcttggtggt
    42961 gaatgcgaag tggatgcgat ttgtgacggt gaaacagtac tcatcccagg gatcatggag
    43021 catattgagc gtgctggtgt ccattcaggg gattcaatgg ctgtgtatcc acctcaaaca
    43081 ttatctgaat cgatcaagaa aacgatcgaa gattacacga tccgcttagc attaggtttg
    43141 aattgtgtcg ggatgatgaa tatccagttt gtcatccatg aaaatgaggt ttatgtgatc
    43201 gaagtcaatc ctcgagcaag tcggactgtt ccattcttaa gtaaggtgac aggaattcca
    43261 atggcacaaa ttgcgacgaa agctattttg ggagagaaac taaagacact tggttatgaa
    43321 aacggacttt atccagaaac aaaagaagtt catgttaaag cacctgtctt ttcttttacg
    43381 aaattgcaga aagttgatac ttacttaggg cctgaaatga aatcgactgg agaagtaatg
    43441 ggatctgaca gacaattaga caaagctttg tacaaggcat tcgaagcttc tggattacac
    43501 ttgcctgaat atggagccgt attatttacg attgcggacg aaacaaaaga agaagcacta
    43561 gaattagcta aacgttttac agaaattggc tatagtctga taggaacgaa acatacagct
    43621 gcttatttcg aaaaaaatgg tctggtcgtt gaaacagtag cgaaaatttc tgaggaagca
    43681 gcagaaaaaa atgtagtcga cttaataaga gatggtaaaa cacaagtggt tgtcaatacg
    43741 attgataaag atcggggaaa tgcttctaaa gacggtttca tcatccgaag agaagcagtt
    43801 gaacatggta ttccattgtt tacttcttta gacacagcag atgcgatcgt tcgagtgatg
    43861 gaatcccgtg ctttttcaac taaccctatt taacggcgag cacatgttaa gacaaaaaga
    43921 cgaaaaaccg ttgcaggagg ggacagatga aacaagaaat gatggaaatc gttcagcaaa
    43981 aacagctggc accaaagatt tttgaaatga cattgaaagg tgatctagta aaagaaatgc
    44041 agcaaccagg acagttcttg catattctcg tgccgcgtgc agacttgcta ttacgccgac
    44101 cgattagctt gaatcatatc gaccacgaaa caaatacttg ccgtatcatt tatcgggtgg
    44161 aaggtgaggg gactagagtc ttctctactc tttctgccgg agaccattta gatgtcatgg
    44221 gtcctttagg caatggtttt gatttgtcca atcttcatac aggagacgaa gtatttataa
    44281 tcggtggagg aatcggtgtc cctcctctat atgagttatc aaaacagtta gtcgaaaaag
    44341 gaattaagcc aacgcatttt ttaggttttg ccacacatga agtcgtttat tatgaaaaag
    44401 aatttaatcg tctaggagaa acaagaatcg ctactgacga tggaacatac ggtatgcacg
    44461 gaaatgtcgg taatttattg ttaggaacga agacggaacc tacagctgtt tttgcatgtg
    44521 gttcaaacgg gttattgaag acagtggaac agctgttttc ttcgcatcaa aatgtccagt
    44581 tatcattaga atcccgcatg gcatgtggga taggggcttg ctatgcttgc gtttgtcata
    44641 cgccggaaga tgaaacagga acaaaaagtg tgaaagtttg tgacgaagga ccgattttta
    44701 aaataggaga ggtaattata tgaatgagaa tcctttagcg gtcaaacttc ctggcctaga
    44761 attaaaaaat cctatcatgc cggcaagcgg ctgtttcggt tttggcaaag aatacggaaa
    44821 atactatgat ttgaatcagc ttggatcgat catggtcaag gcaacgactc caaatgcccg
    44881 ctttggcaat cccacgccaa gagtggcgga aacaccatct ggcatgttga atgcaattgg
    44941 tttgcaaaat cctggtctag acgttgttat gcataaactt cttccagaat tagaggagta
    45001 caatgagtta cccatcatcg caaatgttgc tggtgcttgt gaagaagact atgtagaagt
    45061 ttgtagcaaa attggcgaag caccaaatgt gaaagcaatc gagctgaaca tttcttgccc
    45121 aaatgtcaaa catggcggaa ttgcttttgg aacagattct gatgttgctt ttcaattaac
    45181 acaagcagta aagaaagttt cttctgttcc tgtctatgtc aaattgtccc caaatgtaac
    45241 agacatcgtt ccaatcgctc aggcaatcga agctggaggg gcagatgggt ttacgatgat
    45301 caatactctt cttggtatgc ggattgacct taagacaaga aaaccgattt tagccaatca
    45361 aacaggtgga ctctccggac cagcaatcaa gcctgtcgct attcgtttga ttcaccaagt
    45421 agcaagtata tccagccttc caatcattgg gatgggcggc gtgcagacag tggatgatgt
    45481 tcttgagatg tttatggctg gagcaagtgc tgtagctata gggaccgcga atttcaccga
    45541 tccgtatatc tgtcctaaac taattaaaga gctccctgtg cgaatgagcg aactaggcat
    45601 cgaatcgctg gaaagattaa tcaaagaagt gagagaggaa cgtgaacgat gaatcaacta
    45661 gaccaacgac caattattgc attagatttt tcaactaggc aagaggtaga agactttctt
    45721 cgatttttcc cgaaagaaga aaaattgttt gtgaaaatcg gaatggaatt gttctaccaa
    45781 gaaggacctg aaatcgtacg ttatctaaaa gaggctggtc ataatgtatt tttagatctg
    45841 aaacttcacg atattccgaa tacggtcgaa aaagcaatgc ggggattggc aaagcttggt
    45901 gttgatctca cctgtgttca tgccgctggc ggtattcgta tgatggaggc ggcaatgcga
    45961 ggattagaag aggggactcc agaaggtgga aaacgtccgc tacttctagc tatcactcag
    46021 cttacttcta caagtgaaga agaaatgcat gccgatcaac taatcgaagt ttctttagaa
    46081 aaaagtgtga tccattatgc tagctgtgcc aaaaaagcag gattagatgg tgtggtttct
    46141 tctgcttggg aagtagaagc gatcaaagaa atggcaggag acgaatttgt ttgcttaact
    46201 ccgggtataa gaccagaagg aacagtcgca ggagatcaga caagagtagt cacaccttct
    46261 caggcaaaaa agatcggctc aacttttatc gttataggtc gaccaatcac tcagtcaact
    46321 aatccttata aagcatatca aacgattcaa acagaatgga gctaacctaa aatgaatgtt
    46381 gaacaaagta tcgcaaaaga cttattagaa attgaagcag tatttttaaa tccaagtaat
    46441 ccttttacat gggcaagtgg aatcaaaagc ccaatttact gtgataaccg aatcacgatg
    46501 agctatccaa aagtccgaaa agagatcgca aaaggattag caagcaaaat caaagaagct
    46561 taccctgaag tacaagtgat tgctggaact gcaacagcag gaatccctca tgccgcatgg
    46621 gtagcggaaa tactggattt gccaatggtc tatattcgaa gtaaggcgaa agatcatggg
    46681 aaaggtaacc aaatcgaagg acgtatattt gaaggtcaga agatggttgt gatcgaggat
    46741 ttgatttcta caggcggaag tgtcttggaa gcagctgaag cagcaaaaag agaaggtgcg
    46801 gatatccttg gtgttgcggc tatctttacg tatgagttgc caaaaggaaa agcaaatttc
    46861 gaaaaagccg aaatccctct tatgacgttg accaattatt ctgtattgat tgaagcagct
    46921 ttagaagatc gttatatcga tgagcaagaa ttaactctgc taaaagaatg gaaaaaagat
    46981 cctgaaaatt ggcagattga agcgcgcttg atgaagatag agaaatccga tttgtgtctc
    47041 cagaatcatc aattggaata gcaaaaatcc aaaaactgat tgcgagaagc atcttttcat
    47101 caggtatgaa aagaaacttc tatcgtgatc agtttttgat ttttttaaaa ttcatttttc
    47161 taagactggt tagcagaaag aagagaataa tctcttctct tttttctatt atatatagat
    47221 tttctctatt ttttggtaag ggagctgttt caaaaatggt aaagagcgga aatgttgcga
    47281 atatcagtta tttaatgata tgatattcgg aaaaagaaat ctcaaaggga ggtaactttt
    47341 atggggttga atgaatttat tttaggattg aacaatttag agacgatcac aagggcacct
    47401 ggatttttta aatatacaga gcatacagtt gcagcacatt cgtatcgtgt agcatctatt
    47461 gcgcaagttc taggagacat tgaagaagcg aatggacaac cgatcgattg gaaactgctc
    47521 tatgaaaaat cattgaatca tgactacaca gagcgtttta ttggtgatat caaaacgccg
    47581 gtgaaatacg caagcaaaga gttaagaggg atgctgcaaa cagttgaaga aaagatgacg
    47641 gacgaattca tcaaccaaga gattccagct gagttccaag aagtttatcg ccgtcggttg
    47701 ttcgaaggaa aagatgaaac cgtagaagga gaactattag ccatttcaga taaggttgac
    47761 cttctttatg aatcttttga tgaaatcatc aaaaataacc cggaaaaagt ttataaggag
    47821 atgttcattg aggcagtgac tacgattaaa agctttgaac atcggccttc tgttcagtat
    47881 ttctttaaag agatatttcc ggaactattg aaccaggaat tttatggaaa agaggcgttt
    47941 ttagaaaccg tctcaacgat tgtaaagaaa gaagaaaaat aaaaatgata tgagatgcag
    48001 ggggaagtat agttgaaaaa gaatatggat gtcgaatggg gctattctgg agacaaaggt
    48061 ccggaaaatt gggcgagttt atgtgattgg tttgcgagag gtgcggaatt tccattgcag
    48121 tcgcctattc atttgaccaa gggggaagaa accaaaatag aaggagacac gctttccttt
    48181 cattatcaaa agcaacggtt tactgacaag gaattcaaaa acacgattca tttagtacct
    48241 tttgaccaac tcagttatgt agagttcaag aaagaacgct attatttgac agatattcat
    48301 ttccacttgc cttctgaaca tatcatcaac ggaaaacagg aagatattga atttcatttt
    48361 gttcatatga acagcaaaaa agagaatcta gttgtgggtg tcatgtacca gctaatggta
    48421 caggaaggtt ggctgtataa tcaagaaaat ggggaaagtt ggaacttaga aaaacatgag
    48481 cattgggtaa atccggatgt gttctttccg gagcaaaaaa gccatttcca ttatattggc
    48541 tcattgacaa ctccgcctac cagcgggcca atccaatggt ttgtaatgga tcatgtagga
    48601 aaactgaacc aagagtttct tctccccttc gatggtcagt atgctcgacc gaacaaccga
    48661 ccaatccagc ccttgaatgg aagagaaatt tattatttcg aggagtttga gcaatgaata
    48721 ttcagcagat gaaatacgtg gtcgctgtag caaacaatgg tagtttcaga gaagcggcaa
    48781 agaaaatgtt tgtctcccag ccaagtcttt cagctggtat caaagaatta gaaaccgagt
    48841 tagggatcac gctgtttact cgaacgaatc gaggggcata tttaacagaa gaagggcaag
    48901 acttcttaaa tcgtgccgag aaaatattgg tccagcttga tacattagaa actcactatc
    48961 taacgagtga aaagagagac aatttttcga tcgctgccca gcattatgat ttcttaggtc
    49021 ctttgaccgc acagctactc gaggaattca aagacgagat caaacaattt cggatcatgg
    49081 agacgacaac agcgagagtg atcgaagaag tcaaagagca gcacagtgag atcggcatta
    49141 tttatatgaa tgagcataat caagctggta tcagaagata tttacaacaa ggagagctaa
    49201 gtggccgcag cattggaaaa ttcaaaacgc atattttttt aaggcaagga catccattag
    49261 ctgaacacgc agagatcacc aaagaagaat tgttatctta tcctcaagtt cgttttacac
    49321 aggatggaaa taatttcccg tatttttatg aagaccttat cgagatccct gatcaagaaa
    49381 cagtgattta tacgagtgat cgaggtactt tgatgaatat cgttcttgaa actgatgctt
    49441 acgcttcagg atctggtatt gtgattggag ggattcgcga gcacttgcgg ttaatccctt
    49501 tggcagaagg cgaactaaat gaattttata tcatttattc ggcgaaacgt ccgctcagtg
    49561 aaatcgctca aagatttatc aaagagctta ctcggttact ggaccagcag agctaagaaa
    49621 agcagatgag aacgctgttt attgaacacc gttgatcaaa aaagaggggt tgtgacagaa
    49681 ttttgtcaca actcctcttt ttgtagcaaa caatttataa ctaattgctg ttgtgggaaa
    49741 gtacgcaaat atgattctag aaacactcat tgattacctg ttttgtttca tagctgtaat
    49801 tttttcttct tctggtgtca cgataatcgt cttttggttt tcataaataa cataaccagg
    49861 cttggcgcca tttggttttc ggatatgctt tacctgtaca agatcaaccg gcacttgggc
    49921 agagtatcgg tattttgaaa aataggcagc taactcggca gcttctgtaa ttgtttcatc
    49981 tgagggcttg tcacttttaa tgatgacgtg cgaaccgggt atattttttg catgaagcca
    50041 atagtcggtt tttttagctg tcttcattgt cagctggtca ttttgaagat tgtttttacc
    50101 aacaagaatg agcgttccgt cagaagaaaa gtattgatca ggctgagatg gtttcttacg
    50161 tttttgtttt tttaagttct tctttttcag gtatccttct gaagtcaatt cttctttaat
    50221 tgcttcgata tccattgggc cggcaatttc cagttgagat aagacagatt caagatattg
    50281 gatttcatct ttggcttctt ggatttgttc gccaatcagc ttaacagcgt tcttcagctt
    50341 ttggtatcga tggaaatatt tctgtgcatt ttgatttggt gtcaatgccg gatctagtgc
    50401 tattttgatt ggccgatctt cttcataata attaggtaaa acaacttctt ttgcgccgcg
    50461 tggtacttgt gtcagaaaag tagtaagtag ttcgccattt ctacggtaat tctcagcgtt
    50521 ttcggattct ttgagtgttt gttcacgttt tttcaattta tttttatttc gttttagttc
    50581 attttcgatt tttcgaatca gttctccgcc ttgttgtttt gcccgatctt tctctgcttt
    50641 ttcgtgataa tagctatcta ataattgact gagtgttgga taagctgtta cagcagatgc
    50701 ctgttcggac aatacctgat aagcaatcgg tgtgaaaaaa tctttatttt ccgtttcgta
    50761 aaaagtagga ataggctgat ggctgatggc tgagaaaaat tcgtcccata caaccatctt
    50821 ttcattagga cgttccagta atcttttggt cagctcttga gcgatatcga aaccgattcc
    50881 ctgaaactga cgttggattg cttttggatc tagttcagtt tgggaaagcc gctgaaagat
    50941 tttttctttc tcggagctaa atgggttcaa gacctcttgt tttggcggag caacatattc
    51001 tacaccagga agcaaagaac gataagtgtt ttgagagctt ccaatgtgtt tgatcgcatc
    51061 cagtattttt ccagtttctc gattaactaa gacgatcgta ctatgacgtc ccattaattc
    51121 aacgatcaat atgatatttt gtagatcacc caactcgttt cgtttagcaa agtggaaatg
    51181 gatcactcga tcattttcga tttgttcgat gtcttctaaa atagcgccat ccaaatattt
    51241 tcgaagcatc atcacgaagt taggcggatt atcaggattc tgataatcga tttgggtaat
    51301 ctgtacacga gcataacttg ggtgcgcaga taataataat cgatggtttt tgccgcgtga
    51361 acgaatcacc agcacgattt cattttcata aggctgatgg attttcgaga tccggccaga
    51421 tagtaacgtt tcacgcaatt cgttgaccat cgcgtgggta aatacaccat caaaggacat
    51481 ggttcattcc tcatttcttt tcataagtat attcattata acgaaaaaaa gaagaaaaac
    51541 caatttggcc gaatagtaaa gcaaaagtct agaaaaatct tggactaatt acataaaaaa
    51601 cagtgtttct atcgttgaaa tccaaatttt taggt
//
LOCUS       DARBET010000011        50409 bp    DNA     linear   BCT 07-NOV-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4027
            SAMEA114569841-rid20112183.denovo.011, whole genome shotgun
            sequence.
ACCESSION   DARBET010000011 DARBET010000000
VERSION     DARBET010000011.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA114569841
            Sequence Read Archive: ERR12203666
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 50409)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 50409)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001972618.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001972618.1
            Genome Coverage       :: 132x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/07/2023 13:18:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,041
            CDSs (total)                      :: 2,975
            Genes (coding)                    :: 2,908
            CDSs (with protein)               :: 2,908
            Genes (RNA)                       :: 66
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 67
            CDSs (without protein)            :: 67
            Pseudo Genes (ambiguous residues) :: 0 of 67
            Pseudo Genes (frameshifted)       :: 26 of 67
            Pseudo Genes (incomplete)         :: 28 of 67
            Pseudo Genes (internal stop)      :: 20 of 67
            Pseudo Genes (multiple problems)  :: 6 of 67
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..50409
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA114569841-rid20112183.denovo.011"
                     /isolate="VRE4027"
                     /db_xref="taxon:1352"
                     /country="Denmark"
     gene            <1..375
                     /locus_tag="SCJ86_000757"
     CDS             <1..375
                     /locus_tag="SCJ86_000757"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD hydrolase family protein"
                     /protein_id="HEG1168340.1"
                     /translation="KDAISIYKFFIFIPNRQLKANIFNHLKKMLNVSVTESAPVNIEI
                     VPQNVSKGVVFDRLESIYNLKKPLRIAIGDSLNDLSMFESADISFAMGNSHQEIMKKA
                     DYVTVSNDKDGVSQAFERILTI"
     gene            656..1876
                     /locus_tag="SCJ86_000758"
     CDS             656..1876
                     /locus_tag="SCJ86_000758"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301291.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair exonuclease"
                     /protein_id="HEG1168341.1"
                     /translation="MVKFIHAADLHMDRSFEGLTTLDKTVQEKLLKTNLTVLSNIIEQ
                     AIINNVDFVLLAGDNFHQNRPSLKIQKHFSEQMKRLEKNHIPVFIIFGNHDFYQKERY
                     WFSFPKNVHLFTSETVETKKITIKSGETVSLSGFSYRQPWIQKDKVMEFPSRELTDYH
                     IGLYHGEPGVSQKGNYAPFQPSKMQEKGYDYWALGHIHVPTVLNEKQTIVYPGAPQGH
                     TKKEQASTSILLVELSKGSCQIHPIKVAEVYWKTKEISLRKARTTKEVIVHIRQQLSK
                     VEDGFSLIEIKLKDYDHLNTDVLERIQSGELLDYLLEEFSDRINDFFIWRISLIENTF
                     YTKTPLAASAKLMEQLFQYYQTPQDFQQILNEVYSHQEAARILSELPEYQEETLEKAK
                     QLLNQDFLFEEDQE"
     gene            1873..4593
                     /locus_tag="SCJ86_000759"
     CDS             1873..4593
                     /locus_tag="SCJ86_000759"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631406.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HEG1168342.1"
                     /translation="MKILRIEIAAFGKWRQKSFDFYSGNQLIYGGNEAGKSTIYQFIQ
                     AILFGFPSKGRKKKDYTPKDGSAYGGKIWLKHPVYGEFAVERYKQQNRGKSKVWLGDQ
                     VGSDELLEKLIAPLTKELFQQVFTFQQEQLMELDHLREKELHDALISLGITGSSQVFQ
                     KRQEYYQKAQQLFKKKGQKLLINQKLNEWKELQEKIHQKQEQEAYFGKLIQQEQEYES
                     QQQQLLAQLKEANKQFSLARQQELNFSLYEEWQSLKKNSTTGLPDEQLLLDLESFSKR
                     YQQTNERLEELEATLEKHSGMDQQSARYYFYLEQEQELKDLQQLQFTAEQLNEEWQRL
                     QIETRETNSELKILEQRWHWQKNPLPEEFLYEEEWKHLLAEERRIADLESQISVRLQI
                     AKEQQEMIEAEVNDLEETYPALLQNQSEIAPVSSSKPYMLMAGGAIIIAGIFVPMPLK
                     VILLLIGAGMIGYFFYKRQPKIKTKNDFSEIKGMWQEKLGQLDLALAQLAEAEEKQKR
                     TGEEKQILQQEVYRFADHRHLGKMDTLKLMKEYGQEVIDYQKLYGVFERKHKREQEIQ
                     EKLRQIDQKFEFVKEWLPIQDKTISEKMVLLSRFIQEMEQMKFARSYQQNTLLKQEIN
                     QLKKKQKVWIQEYSEQLTQAGLSYPSEVPVFLQKAKQAEQQMKRYNELSEMLTPLFPE
                     ELTKEKLSVRLRQLKEKQSQLQQEEKEASENRQRLQLQVEELQRDGTLDELYQQASRQ
                     KAKLQELLEEWGALEIAGTFLGDLSTEMSERQLPQLLRAAGDYLGILTKGNYQKILLK
                     NDTLTLTDGYTEFPIYELSTGTKDQLIMAMRFAYLSLEANRSICPIIIDDGWLHYDHQ
                     RKYQLAELLAKFGEKYQIICFSSDKEMVSYYHEMNQIVKQLEGANNEKNS"
     gene            4577..5521
                     /locus_tag="SCJ86_000760"
     CDS             4577..5521
                     /locus_tag="SCJ86_000760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373023.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing protein"
                     /protein_id="HEG1168343.1"
                     /translation="MKKIRDLVIDEQFEQFVLIKSADVRIAKNGKKFIAFTFQDTSGT
                     IDGKYWDASEEEINKFTAGTVVFLNGKREVYQGNPQVKILHMRLTRVDEPGEPSLYME
                     RAPLKKEEMEEEINQLLFEITNAHWNRIVRYLLNKYQKQFFEFPAAKRNHHAFASGLA
                     YHTLTMLHLGKSIADEYPELNRALLYAGIILHDLGKVIELSGPMSTEYTLAGNLIGHL
                     VLIDEEITKACLALKISEADEDVLVLRHMVLAHHGLLEYGSPVRPRIMEAEILHQIDN
                     IDASMQMMLTSIRQTEPGKYTDRIFGMDNRSFYVPKDL"
     gene            complement(5590..6600)
                     /locus_tag="SCJ86_000761"
     CDS             complement(5590..6600)
                     /locus_tag="SCJ86_000761"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332217.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HEG1168344.1"
                     /translation="MKKKSIILAATSALAVLTLAACSGDTNKDIATMKGGTITVSDFY
                     DEAKLESSNQSLVQRMIIYKVFNNKYGDKVTDKQVDAEYDKQAKSLGDTFESQLEAAG
                     YTKDTYKEYIRNNLAFEAGLKAHVDITDDDLKTAWKSFHPEVEAQIIKLSSEDEAKDV
                     KKSADDGDDFSKLAKDKSTDTETKEDGGKVKFDSTTTTIPAEVKEAAFKLKDGEISDV
                     ITTTNPTSYATEYYVVKMVKNQNKGNDMDKYKDQLKDIATETKLSDNAFTTKVIGEEL
                     KDANVKIKDDAFENVLSAFTTTSSSTKDSSETTASTKSSDTKSTDSTKESSTEETTDS
                     SK"
     gene            complement(6780..7124)
                     /locus_tag="SCJ86_000762"
     CDS             complement(6780..7124)
                     /locus_tag="SCJ86_000762"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YtxH domain-containing protein"
                     /protein_id="HEG1168345.1"
                     /translation="MIKNFFKGLLFGAAAGTVGGLLLAPRSGNETRKKLIEELEEATT
                     LTNDLNNSLNHFKSALIETKETAESVIPAFQEAIKKDIEDFKFQAEPRITQINEQVEK
                     LKADLPEYPEQI"
     gene            complement(7117..7551)
                     /locus_tag="SCJ86_000763"
     CDS             complement(7117..7551)
                     /locus_tag="SCJ86_000763"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HIT family protein"
                     /protein_id="HEG1168346.1"
                     /translation="MENCIFCKIINQEIPSYKIYEDDKVYAFLDISQATKGHTLVVPK
                     RHVADIFEYDPELAGEVFSRVPKIAQALEKAFPEMKGLNILNNNRELAYQSVFHSHIH
                     LVPRYSKEDDFSIHFVNHQDSYGSEELKAIQETIVKQVSCDD"
     gene            7689..8426
                     /locus_tag="SCJ86_000764"
     CDS             7689..8426
                     /locus_tag="SCJ86_000764"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286199.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEG1168347.1"
                     /translation="MSLIIDHVTGGYGHLPVLKDISFDVQPGEIVGLIGLNGAGKSTT
                     IKHIIGLLEASKGTIEINHLSLKKNTEEYRKQIGYIPETPSLYEELTLREHIEVTAMA
                     YDIPMDIAMKRAEKLLETFRLSNRLDWFPANFSKGMKQKVMILCAFLIEPSLYIIDEP
                     FLGLDPLAINALLELMVEMKKEGAGILMSTHILATAEKYCDRFVVLHNGEIRADGSLE
                     MLRKEFQLPDSSLDEIYIALTKEQAHE"
     gene            8419..9630
                     /locus_tag="SCJ86_000765"
     CDS             8419..9630
                     /locus_tag="SCJ86_000765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373028.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEG1168348.1"
                     /translation="MSSFFQKRLARHQKKMMKYMRYVLNDHFVLVCLFLIGGVGLYYS
                     NWLKTLQPPFAIGGLIISVFWMLCLFVGKMATFAEAADIVFLLPKEKEMQTYLERAFR
                     YSCIFPLIFLILACGLAMPLVVVSTEQSFSMFFVYLIILWSLKFSHLQIKRAELFRLD
                     QQVIRYWKLGWFFSSLIFLFLSFYWLIWAGIAGALVQASLYYWFLWKQQEKGLDWEKM
                     VHVEEHRLHQIYQFINLFTDVPEITAKIKRRRYLDPILTHIKRESKNTYLYLFARRLA
                     RGSDFSGLYFRLVIIGGLLIAGVVDFYFSLIIGFLFLYLIGFQLLPLANQFRYMVLVQ
                     LYPIHKNQKKQAIQYLLTWVLIIASVIFGLIAAVVLDGQQRWVPLAAYLIVTMLFVKL
                     YVPTRLKKMSD"
     gene            9834..10616
                     /locus_tag="SCJ86_000766"
     CDS             9834..10616
                     /locus_tag="SCJ86_000766"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010750310.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphotransferase family protein"
                     /protein_id="HEG1168349.1"
                     /translation="MMEFQLDQEWRLRPIKGDTGKTYIGMRDEDKVFIKRNTTPMLAA
                     LSREGITPKLVWTKRTGNGDTLTAQEWLEGRLLTPNEIGQRNDVIDVLYHLHHSNSLK
                     TMLTRIGGRVMTPEKMLQKYEKELPEELQKNQFLQIVHRFLREQIPSIDKENLSVVHG
                     DVNHRNWLVCKNYLYLVDWDSIMFADPAVDIATILGNYVPLSSWNQWLISYGIRPTDE
                     MLEKFHWYAAMNLLQEITRYCKREDHRRMNEEILQLKRIFSG"
     gene            10686..11348
                     /gene="trmB"
                     /locus_tag="SCJ86_000767"
     CDS             10686..11348
                     /gene="trmB"
                     /locus_tag="SCJ86_000767"
                     /EC_number="2.1.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373031.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (guanosine(46)-N7)-methyltransferase TrmB"
                     /protein_id="HEG1168350.1"
                     /translation="MRVRNRPGAAEMLAAHPNFVISDPTLWKGKWNELFENDHPIHIE
                     IGMGKGQFITGMAKAHPEINYIGVEMQVSVVSIALDKLIEQPLPNLKLLHVDGSALTE
                     YFADSEVDQIYLNFSDPWPKKRHEKRRLTYKTFLAVDEQILRPNGEIHFKTDNQGLFE
                     YSLASFSQYGMILKQVWLDLHQSQFEGNIMTEYEEKFSSKGQRIYRVEAQFQDKTKKK
                     SL"
     gene            complement(11446..13200)
                     /gene="ade"
                     /locus_tag="SCJ86_000768"
     CDS             complement(11446..13200)
                     /gene="ade"
                     /locus_tag="SCJ86_000768"
                     /EC_number="3.5.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008268838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenine deaminase"
                     /protein_id="HEG1168351.1"
                     /translation="METTLRKYIEVASKKKPADLVIKHAKIVNVFTKEIMEKDVAICE
                     GMIVGIGEYEGKSVHNANGQYLVPGFIDGHVHIESSLLSPSEFSKVSLLHGVTTVVTD
                     PHEIGNIAGSTGLEFMIEDARQTLMNIFFMLPSCVPVTPFETNGANLNAKKLAPFLQK
                     PEVLGLAEVMNYQAVANNETDILEKIQLMHQHKKKIDGHAAGIGMEELNVYPAAGIRT
                     DHEATTAKEAKERLDLGMYLMVREGTVAKDLASLLPAITPENSRRCFFVTDDKLINDL
                     VKEGSIDHIVRQAIALGIDPLQAIQMATLNAAECFDLKTLGAIAPGYQADFFLTDDLT
                     KLPIHEVFYKGTLVVQSGQLQDHLFADHSNSYTCCLPKLNAQPLNPHSLELLLSSSQA
                     HVIEIIPNSLVTKDLVEKVDRQGERFIPSIEKDQLKIAVIERHHQTGNIGLGIVKGFQ
                     LKQGAIATTIAHDSHNLVVVGTNDEDMLYAANLLIQKGGGMTVVNKQQEIACVSLPIG
                     GIMTQEPFSIVYEQLNELITKAHQLGAAKTFDPFLTLSFLTLSVIPELKITDKGLFSF
                     SRFQLIPPCGKTDEINTF"
     gene            13332..14261
                     /gene="tadA"
                     /locus_tag="SCJ86_000769"
     CDS             13332..14261
                     /gene="tadA"
                     /locus_tag="SCJ86_000769"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346292.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Flp pilus assembly complex ATPase component
                     TadA"
                     /protein_id="HEG1168352.1"
                     /translation="MEIKRLSDQMISESFAKHAQDVYILPASEVFYIYFRYGKKRTFW
                     KKVSSADGQQLISRFKYLGQMDIGEKRKAQLGAFTYSLEKQEVRLRLSSVGDYLQRES
                     LVIRLLYGTFETPHRCFSSRDFATILQKTVRRGLYLFSGTVGSGKTSLMYRLALEEKL
                     QVITIEDPVEIEEPFFLQLQINPKIAQTYDTLLKLALRHRPDLLIIGEIRDESTAHAA
                     VRAALTGHRVFATVHARNLEGTLVRIHELTKKKEELSECLAGVIYQELLEDANHHPSV
                     LWSYQFMEEESVKKSWEESYEEAQENIWQSELF"
     gene            14218..15267
                     /locus_tag="SCJ86_000770"
     CDS             14218..15267
                     /locus_tag="SCJ86_000770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628374.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="competence protein ComGB"
                     /protein_id="HEG1168353.1"
                     /translation="MKKLRKIFGSQNYSKGLTRSQQNQFAQTMGELLLSGFSLQESLY
                     LLLTIRSFPPVILANIQHQLKEGQAFSEVLSQIGYGAEQLVQIELAERHGNLPTTLLE
                     IAKQFRLVEGFRNDLKKLLAYPMLLLTFLLGILFSMRLFVLPQLIGSGMVMQEHWGIQ
                     LIQVFHWYVLLFILAVTLLALLVRYRLMKLSPVDQSEWISRQLLIGPIYSLYQSAYLS
                     LEIGKLFHEGFELRQIIVCLKETKQESLIQHLASKLIQGLEAGMSLAEQFQNYRFLSE
                     EFSKIVLQGEAKGSLGKELIFYSEWTRKQLFKRLHHWMHFIQPIIFLGVAALILLVYA
                     AVLLPVYGSIEEVLP"
     gene            15264..15542
                     /locus_tag="SCJ86_000771"
     CDS             15264..15542
                     /locus_tag="SCJ86_000771"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin-type N-terminal cleavage/methylation
                     domain-containing protein"
                     /protein_id="HEG1168354.1"
                     /translation="MKKRKISYYSGFTLIEMLIVLLIISVLVLLFVPNLSRYRNHVDQ
                     ESREAIIQLVDTQKELYALQNNGRVPTVEELLNEGYIKREHAEIYQRP"
     gene            15517..15987
                     /locus_tag="SCJ86_000772"
     CDS             15517..15987
                     /locus_tag="SCJ86_000772"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628375.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system protein"
                     /protein_id="HEG1168355.1"
                     /translation="MQKSISGLEGKVLASGYTLFESLLLILLLTTILSFPVLAFSAWK
                     NELAVYQFFGQFEKRIYSTQKIAIVNQIQTGFYWNSEENQIIFHVPNPDKINWEVLDI
                     PDEITLKRHASITFAARTGNESSLKAYQFYWEEKERTITYQFQIGSGRYTKRIE"
     gene            16004..16306
                     /locus_tag="SCJ86_000773"
     CDS             16004..16306
                     /locus_tag="SCJ86_000773"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEG1168356.1"
                     /translation="MESLTALMIASVCVYLVSFGQLFLLQAEKKSGEELLFIRMIYED
                     IKTQRMYHKRPERREEMIPYDIQIVDNNGVIEKVFVNMEKEAISIEKTSNSSIYAD"
     gene            16323..16694
                     /locus_tag="SCJ86_000774"
     CDS             16323..16694
                     /locus_tag="SCJ86_000774"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286176.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ComGF family competence protein"
                     /protein_id="HEG1168357.1"
                     /translation="MILSSLLLTFNLLIQQTIQVSEFLQRKDQKEWLIFLAQLEEELK
                     SSHNVSVKNQQLHYTIEDKQYIIERYQAMIRKRRTSGGHQPMLTSITELQLLEKEHTI
                     SFYVHFENGEDGYAIWTKNDQ"
     gene            16666..17025
                     /locus_tag="SCJ86_000775"
     CDS             16666..17025
                     /locus_tag="SCJ86_000775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324245.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEG1168358.1"
                     /translation="MPYGQKTISRKCYKGSILFSVLLLFLLCTFLLMAALESYRLSMD
                     LNIRIKNYYIAKIMKQMALSEQSTLAESSEGVFYYTTGSVTYQWVQQSLFLEVEVSPF
                     IFRFTEEVKNDTDTSTE"
     gene            17197..18204
                     /locus_tag="SCJ86_000776"
     CDS             17197..18204
                     /locus_tag="SCJ86_000776"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HEG1168359.1"
                     /translation="MLPEKMEQGFNQNLEAIQLLQNALGTSFLEAYVENAENLIDDYQ
                     VRVVDGVPTKETTQRITALYEELKKLSFEPEEWRRLSQLLLLKGSQTEHLQANHQLTP
                     DSIGFLFVFLIEQLYTNKKAPVKILDIAAGMGNLLLTVLLNLSNAGYQTEGIGVDIDD
                     TLLAVAASTSDLTQANVQYFHQDGLQELLIDPVDFAISDLPIGYYPNDEKAKEFLTST
                     EEGHSYAHHLLLEQSMKYVKPDGFGLFLMPSGFLETDQSEEIKKWFKEEGYLQGMIQL
                     PDELFRNKQSQKSILILQKKGPQAQQVKEVLLVKLASLKEPEKVTEFFNEFKNWKSSA
                     L"
     gene            18229..19413
                     /locus_tag="SCJ86_000777"
     CDS             18229..19413
                     /locus_tag="SCJ86_000777"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328121.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetate kinase"
                     /protein_id="HEG1168360.1"
                     /translation="MSKTIAINAGSSSLKWQLYQMPEETVVAKGIVERIGLQDSIFTI
                     KYGDNQKFEQIMDIENHEVAVKMLLDQLIELQILSSYDEITGVGHRVVHGGETYGDSV
                     VIDEKVMDRIAELAEFAPLHNPANLMGIKAFKKILPDILSVAVFDTSFHSTMPKQNYL
                     YSLPVSYYEDFKVRKYGFHGTSHRYVSERAAKLLNKPIEELKIITCHLGNGVSITAVD
                     GGKSMDTSMGFTPLAGVTMGTRSGDIDPAVLPYLMEKLEIDIDEMINVLNKKSGLLGL
                     TGLSSDMRDLEKNYEKEHIRLAYDIFVDRIRKYIGGYVTTMNGVDAIVFTAGIGENDG
                     HVRSEIIKGMSWFGCEIDPALNELRGEEVDISTKESKVRVLVIPTDEELMIARDVERL
                     RG"
     gene            complement(19850..21433)
                     /locus_tag="SCJ86_000778"
     CDS             complement(19850..21433)
                     /locus_tag="SCJ86_000778"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738640.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F family ATP-binding cassette
                     domain-containing protein"
                     /protein_id="HEG1168361.1"
                     /translation="MIIQAISIKKTIGGNPLFEDLSCQIEAGEKIALVDINGTGKSTF
                     LQILTGREGVDSGVISRKKGLKIGTVEQELTVSEATVNHYLLHSAAEIQDLKQQMSRY
                     EALMTEPDTNLEKCLARYGELQHRFEELGGYVLEDRIAAILQGLGIPHCQEARLTELS
                     GGERVKVALAKILAADADLLLLDEPTNHLDLNSIRWLENYLQNTKKSVLVVSHDRQFL
                     DQVTTKTWELEEGALIEYPGNYSRFRVLKEARLAELTKNYELQQKEVQRLKVMIRRFR
                     QWAHEGDNESFFKKAKELERRLAKLTLVKPPPPPKNRLQSLSNGGKSGKEVFIIQNLH
                     QQYADQVLFKDSSFAVYRGDHLAIIGDNGAGKSTLLKLLLGEEKPYGGTIKLGSSLQI
                     GYLPQQLQFADPDSRLLAYAITLTGNEEVARRQLAKSGFYQSDVGKRIKDLSGGEKIR
                     LALLKLFLEKINVLILDEPTNHLDSYAREEIEEMLQDYQGTLLAVSHDRYFLQQHFQE
                     ALVIDQEQISRQPLGQLID"
     gene            complement(22011..22154)
                     /locus_tag="SCJ86_000779"
     CDS             complement(22011..22154)
                     /locus_tag="SCJ86_000779"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293650.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="portal protein"
                     /protein_id="HEG1168362.1"
                     /translation="MEVSDESDTKKYEVSMADTDFIAKLKFYFGFGYREKASATFGKS
                     FKN"
     gene            complement(22376..22855)
                     /locus_tag="SCJ86_000780"
     CDS             complement(22376..22855)
                     /locus_tag="SCJ86_000780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337968.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein"
                     /protein_id="HEG1168363.1"
                     /translation="MLQQYKKIMVAVDGSDEAELAFKKAVNVAIRNNGELLLAHVIDT
                     RSFQTVSSFDGMLAEQATEMAKQTLADYESNAKKAGLNNVTSVVEYGSPKQIIAREIP
                     EDNQVDLIMLGATGLNAVERLFIGSVSEYVIRNAACDVLVVRTDLENQLPAKDHTKA"
     gene            complement(22928..23212)
                     /locus_tag="SCJ86_000781"
     CDS             complement(22928..23212)
                     /locus_tag="SCJ86_000781"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEG1168364.1"
                     /translation="MIRILMIIATLVLLFVSYYLFNKQDIFFVLINKNDKNQGFLQFY
                     GAAYAVLGVMGILTAFFNQRFIALVFLLIVIIVSATFSIRFAKKIAEPKQ"
     gene            complement(23290..23484)
                     /gene="ssrS"
                     /locus_tag="SCJ86_000782"
     ncRNA           complement(23290..23484)
                     /ncRNA_class="other"
                     /gene="ssrS"
                     /locus_tag="SCJ86_000782"
                     /product="6S RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00013"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00013"
     gene            complement(23556..24842)
                     /locus_tag="SCJ86_000783"
     CDS             complement(23556..24842)
                     /locus_tag="SCJ86_000783"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341136.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication-associated recombination protein A"
                     /protein_id="HEG1168365.1"
                     /translation="MQQPLAYRMRPRTIDEVVGQQHLVGEGKIIRRMVDARMLSSMIL
                     YGPPGTGKTSIASAIAGSTNYAFRMLNAATDSKKDLQVVAEEAKMSGTVILLLDEVHR
                     LDKTKQDFLLPHLESGRIILIGATTENPYITINPAIRSRTQIFEVKPLTEQDIQLAVE
                     HALRDKERGLGQQAIQLDEDALLHLSRATNGDLRSALNGLELATLSTPSDKEGRIHLT
                     LSIIEECVQRKALTHDKNGDAHYDVISAFQKSIRGSDVDAALHYLARLVEAGDLASIC
                     RRLMVIGYEDIGLGNPAAAARTVNAVLAAEKLGLPEARIPLADVVVDLCLSPKSNSAY
                     MALDAALADIREGKAGDVPDHLRDSHYKGAKSLNRGVGYQYPHHFDQAWVNQQYLPDK
                     LKNAQYYQPKDTGKYEQALGQQYYRIKEWKKHPPKN"
     gene            24980..25651
                     /locus_tag="SCJ86_000784"
     CDS             24980..25651
                     /locus_tag="SCJ86_000784"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289090.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-3-methyladenine glycosylase"
                     /protein_id="HEG1168366.1"
                     /translation="MTDKQKIDEFFWNKTTPEVARDLLGMYLEHDTPEGRLAGYIVDA
                     EAYLGPEDEAAHSYGLRRTPRVRAMYEKPGTIYLYTMHTHRILNIITQPEGIPQGVMI
                     RAIEPAAMIDQMSKNRGGKTGPDISNGPGKLVEALAIPQELYGQSIADSSLRLVFEKK
                     KTPKKINALPRIGIPNKGVWTEKPLRFVVSGNPYISLQRKNQIEKDWGWRKENEKEGS
                     INIFR"
     gene            25617..26105
                     /locus_tag="SCJ86_000785"
     CDS             25617..26105
                     /locus_tag="SCJ86_000785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629576.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3013 family protein"
                     /protein_id="HEG1168367.1"
                     /translation="MKKKDLLTYLDEIIEKQVTDYDVALDWDTKNHTIEIVVRLFAEN
                     KAQFEIDDAEGIVSQEPIIEFEDGVLLFNPQKSVFDERNYLAVIPYEGKKGLAKSVAD
                     SLVEYLNEVLAQGQSDLLDFLDEDDDEAVFELHWDNQQLAELVEVKQQNEADTYLPYP
                     SY"
     gene            26118..27065
                     /gene="prmA"
                     /locus_tag="SCJ86_000786"
     CDS             26118..27065
                     /gene="prmA"
                     /locus_tag="SCJ86_000786"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010737084.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L11 methyltransferase"
                     /protein_id="HEG1168368.1"
                     /translation="MKWNEVKVETESEAVEAVANILMEAGASGVAIEDSLDVEHFQSD
                     QYGEILDKNDFTSLKEGALVMAYFPETTFLPEILPFIKDRITQLPEFGLAIGKNKVTV
                     SEVEESNWATAWKKYYHPVRITRMLTVAPSWENYQTEDPLEKIITLDPGMAFGTGTHP
                     TTSLTLTALETVLRGGETLLDVGTGSGVLSIAAKYLGAKDVYAFDLDEVAVRSAKENM
                     DMNEIAKDVHVSANDLLTGIEIESDVIVANILADIILLMIPDAWRLLKQTGTLIVSGI
                     IEEKKQMVLDAMLEQGFIVDQIFQQKDWYAIILKKPEVE"
     gene            27066..27830
                     /locus_tag="SCJ86_000787"
     CDS             27066..27830
                     /locus_tag="SCJ86_000787"
                     /EC_number="2.1.1.193"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (uracil(1498)-N(3))-methyltransferase"
                     /protein_id="HEG1168369.1"
                     /translation="MQRYFLKESYESKDYFVLEGENYHHIVRVMRMEPEDEVFLAFND
                     QIAIVAEITEITEEAVYLKEIKKEENQKELPSSITIASGYPKGEKLDWIVQKSTELGA
                     QAFLGFPASASVVKWDAKKRMKRQQRLEKIAQEAAEQSHRQIVPSVELLETKQDLFER
                     LDDYDVVLVAYEESAKQGEKSNAAKILSETKPDSRILAVFGPEGGLSRDEVDYFVEHK
                     GILCGLGPRILRTETAPLYLLSAASYQWELMNPSFS"
     gene            28063..30276
                     /locus_tag="SCJ86_000788"
     CDS             28063..30276
                     /locus_tag="SCJ86_000788"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016616266.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional (p)ppGpp
                     synthetase/guanosine-3',5'-bis(diphosphate)
                     3'-pyrophosphohydrolase"
                     /protein_id="HEG1168370.1"
                     /translation="MTKEEILTGPGVIKLVSQYMGPEHVAFVEKACEYATAAHDGQFR
                     KSGEPYIIHPIQVAGILADLKMDPHTVATGFLHDVVEDTEITLEDLREEFGDDVAMLV
                     DGVTKLGKIKYKSHEEQLAENHRKMLLAMAQDLRVIMVKLADRLHNMRTLKHLREDKQ
                     RRIAQETLEIYAPLAHRLGISRIKWELEDTALRYLNPKQYYRIVHLMQTKREEREKYV
                     SGTVEDIRIATEELGIFAEIYGRPKHIYSIYRKMKDQKKQFNEIYDLLAIRVIVDSIK
                     DCYAVLGAIHTKWKPMPGRFKDYIAMPKANMYQSLHTTVIGPAGNPVEIQIRTQEMHE
                     IAEFGVAAHWAYKEGKNEKVEPDGMTKQLSWFHEILELQDESYDASEFMEGVKGDIFS
                     DKVYVFTPKGDVTELPKGSGPLDFAYSIHTDIGNKTTGAKVNGKMVQLDYKLKNGDII
                     EIMTSPNSFGPSRDWLKLVATSKARNKIKRFFKAQDREENVIKGHESVVKCITDLGFT
                     PKDILTKNKLQEALDRFNYQTEDDLYAAVGYGEVSPLTMANRLTEKERKEQKIEQQKQ
                     EAEEIMNQPKKEPDKMKVRHEGGVVIQGVENLLIRISRCCNPIPGDDIVGYITKGRGI
                     SIHRRDCPNVQPDKPNVAERLIEVEWEDTSNTRKEYDADLEIYGYNRSGLLNDVLQTV
                     NALTKNLNSVEARTNKDKMATIHLTVGIQNLSHLKSIVDKIKAVPDVYSVRRTNG"
     gene            30287..30733
                     /locus_tag="SCJ86_000789"
     CDS             30287..30733
                     /locus_tag="SCJ86_000789"
                     /EC_number="3.1.1.96"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-tyrosyl-tRNA(Tyr) deacylase"
                     /protein_id="HEG1168371.1"
                     /translation="MRAVVQRVSKAAVSVDQQEIGKIGYGLLVLLGIHETDTQKDVDY
                     LVKKIAQMRIFEDEQGKMNISVEDVGGQILSISQFTLFADTKKGNRPSFVEAARPETA
                     IPLYESFNEGIRSRGITVATGQFGADMSVSLVNDGPVTIIIDSKNK"
     gene            30904..32163
                     /locus_tag="SCJ86_000790"
     CDS             30904..32163
                     /locus_tag="SCJ86_000790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289099.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome P450"
                     /protein_id="HEG1168372.1"
                     /translation="MKEVPVVDIKITDLKKLYQKGYNMLEELRHEADAPVVKAKIFNK
                     EAITIYGSSAAKVFYDPRNFKRKGAMPKLVLKTLFGQGGVQTLDGAAHHHRKNIFMDL
                     MTPERMEDYHRILDKNLTQALEAQHGQFELFDLSKMVFFTSICEWAGINLSAISKDEV
                     EKLAEYQISMISGTFTSPIDHIKGVENRKKSEKWAQGLIEEARQNPVAGKENVALYAF
                     ANATDLDGQLLPLEVAAVELLNIIRPTVALTVWAALMGHALFSRPDLYQQLKNDFSTL
                     QDPFIQEMRRYYPFFPMLPAISLKEVEVDGYRIPEGSWVILDLYGTDHDERTVEAPDS
                     FMIKRYVGKAKDISYKEEYEMIAQGGGNFRQMHRCAGEWITLHSLRVFSDQLVNKFEF
                     SVPEQDWTIPFNQFPTYPNSRALLYKN"
     gene            complement(32252..33199)
                     /locus_tag="SCJ86_000791"
     CDS             complement(32252..33199)
                     /locus_tag="SCJ86_000791"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325318.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HEG1168373.1"
                     /translation="MAEIRIGHSQVYAEQLGLGANAVGGHNLFDGLEDETGKQVVRTA
                     LNSGINLIDTAYAYGNGRSEELIGEVLKEKEYDRSRVVIATKAAHVPNKGRTFDNSPE
                     FLKQSVEDALKRLQTDYIDIFYIHFPDESTPKNESVATLHELKEAGKIRAVGVSNFTL
                     EQLKEANADGYVDVVEDKYSLIHRQAEKELFPYLEKNKISFVPYFPLASGLLTGKYEL
                     GEEKQFGEGDPRKRNPDFQGERFREILTAVDVLRPIAKRYQATPAQLVLAWYMKNPRV
                     SVVIPGAKRPEQVSDNVQALDLHLSNEDYQTIDEAFRGF"
     gene            33593..34885
                     /locus_tag="SCJ86_000792"
     CDS             33593..34885
                     /locus_tag="SCJ86_000792"
                     /EC_number="6.1.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005877310.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine--tRNA ligase"
                     /protein_id="HEG1168374.1"
                     /translation="MSFQRPKGTNDILPGESEKWQFVEETARLLFRDYQYNEMRTPIF
                     EHYEVISRSVGDTTDIVSKEMYDFYDKGERHVTLRPEGTAPIVRSFVEHKLFGPEYAK
                     PFKTFYMGPMFRYERPQKGRLRQFHQIGVEAFGSENPATDVESMVMALDFFKQLGITQ
                     IRLVINSLGDKETRQAYRQALIDYLLPFESELSEDSKRRLHENPLRVLDSKDKRDQKF
                     VAEAPSILDYLSEPAQKHFDTVVSMLDALAVPYEIDSNMVRGLDYYTHTIFEIMSDAP
                     KMGAQATICAGGRYDNLVEELGGPSTPGFGFAMGIERLLLTMEAEEVVIPAFNELDAY
                     VVALGDETNIEALKVVQAIRNFGFSADRDFMNRKAKAQFKTADKANAKLVLVIGEDEL
                     ASQTINVKAMASRKEKSFPLEEIYEHFDEVYDEMTLFE"
     gene            34900..36669
                     /gene="aspS"
                     /locus_tag="SCJ86_000793"
     CDS             34900..36669
                     /gene="aspS"
                     /locus_tag="SCJ86_000793"
                     /EC_number="6.1.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307075.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate--tRNA ligase"
                     /protein_id="HEG1168375.1"
                     /translation="MAQRTIYCGLVSEQQLEQTVTLKGWVQKRRDLGGVIFIDLRDRE
                     GIVQIVFNPEINKEAWEIADKCRSEYVIEVTGEVRRRGENAVNPKMATGEFEVIASEI
                     VILNQAKTPPFQIEDDQAISDEIRLKYRYLDLRRPKMTKNIKLRNATTKAIRHYLDDN
                     DFLDIETPYLGKSTPEGARDYLVPSRVHAGHFYALPQSPQLFKQLLMNAGFDRYYQIV
                     RCFRDEDLRGDRQPEFTQVDIETTFLTAEEIQTYTEGLIAKVMKEVRGIEVTLPFPRM
                     TYDEAMARYGSDKPDTRFDMELIDLSDTVKEVEFKVFQMALENGGVVKALNAKGAADR
                     YSRKDMDQLGQYVGQFGAKGLAWLKVEEDGLKGPIAKFMGEATEAIIKATDAKPGDLL
                     MFGADKSEIVAAALGAIRTRLGKELGLIDESKFNFLWVTDWPQFEFSEEEGRYVSAHH
                     PFTMPKEEDIPLLAEDPAKVYAEAYDIVLNGYELGGGSIRIHTRELQEKVLETLGFTK
                     EQMEEQFGFLLTALDYGFPPHGGIALGLDRFAMLLAGEDNIREVIAFPKNGKAADVMS
                     DAPSMVSPLQLFELNIDVTNIEE"
     gene            36778..37653
                     /locus_tag="SCJ86_000794"
     CDS             36778..37653
                     /locus_tag="SCJ86_000794"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YitT family protein"
                     /protein_id="HEG1168376.1"
                     /translation="MKKTFENWAYHDYTTKLSVSIVYALIASVALNFFYQPGHIYSSG
                     ITGLAQILTTLSKSLIGFEVPVSTTLYLLNIPLFVLAWLKIGKKFTIFTFITVTLTSL
                     CIHILPETVLSTDPIICALFGGAVMGLGIGLALRNGLSSGGLDIVSITIRKKTGKSIG
                     SISIYFNALIVFVAGILFGWQYMFYSTLSIFVSGKVTDVVYTKQKKMQVMIVTKHPEK
                     VVNEIQNCMRRGITIINEAEGAYKHDKQTVLMTIVTRYELPLLREAMKASDPQAFVSI
                     AENVQILGRFYEDGI"
     gene            37919..39802
                     /locus_tag="SCJ86_000795"
     CDS             37919..39802
                     /locus_tag="SCJ86_000795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyltransferase"
                     /protein_id="HEG1168377.1"
                     /translation="MEKQKRLKNSRYITGFDGLRSLAVVGVIFYHLLPTSMKGGYLGV
                     PIFFVVSGYLITDLLRQEWEQNGKINIWQFYVRRMKRLYPALVFMLVTASAYITLFQR
                     GLLNNLRGVVVSSLLYVNNWWQINNGLSYFDRFANESPFTHIWSLAVEGQNYLVWPIL
                     FVLLMVFVRKRKWIFYTVLGTSFLSAILMMILYTPGGDPTRVYYGTDTRLFSIWLGSA
                     LAFVWPSTRLKKNIPKQAKNILNIAGGISLLALILCFFFLDDHYNFIYYGGMLLVSLL
                     CVVLVAVTAHPGASLNRWLTNPVFTWIGKRSYGIYLYQFPVMIFYEAKVTDIADHVLL
                     HTLVEITLILIISELSYRYVERPLARFDYGRTFQVMKGWFSKPIISKSKPWVVPGTLL
                     VAIALVGFVTAPKNAVTADQKQLQAKIAESKKLAEETRNNSENTTSSVDQAILDKYEL
                     SKAQGNKAQKLELTAFGDSVMLDAATDLQELYPKVVVDGDVGRQLYASVPYIEKLKEQ
                     DLLKDTVLIGLGTNGSFSEAQFDSVMDAIGDRKVYWVNVRVPTKRWQNDVNAMLEQMT
                     KKYDNMTLIDWYSYSNDHSDWFYDDQVHPNPEGMTHYIHLVSEALLGGSSSTNSSETN
                     VTE"
     gene            complement(39877..40161)
                     /locus_tag="SCJ86_000796"
     CDS             complement(39877..40161)
                     /locus_tag="SCJ86_000796"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304776.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GIY-YIG nuclease family protein"
                     /protein_id="HEG1168378.1"
                     /translation="MANEHYFYVLFCKDQTFYGGYTTDLSRRLEEHNNGTGAKYTRLQ
                     KRRPVKMIHAEVFSSRSEATKAEAAFKKLTRKQKERYLQTHSTVLLPESI"
     gene            complement(40151..40885)
                     /locus_tag="SCJ86_000797"
     CDS             complement(40151..40885)
                     /locus_tag="SCJ86_000797"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341140.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA1(Val) (adenine(37)-N6)-methyltransferase"
                     /protein_id="HEG1168379.1"
                     /translation="MLHEDERIDQLYAEDIKIIQSSKVFSFSLDAVLLAHFSRVPKKG
                     KIVDLCAGNGAVGLFLSKRTQAAIDAIELQPRLADMARRSIALNHLNEQMTVHTIDLK
                     DSLSVVRHNSCDLVVCNPPYFKGLPTNKTNPNKHLAIARHEIHTTLEEVIDISSKLLK
                     TNGRFTMVHRPDRFLEIIQVMEDYRIAPKRIQFVYPKPGKEANILLVEGIKEGKLDGF
                     KVAPPLITYNMDGEYTAEIREMLYGK"
     gene            41014..41637
                     /locus_tag="SCJ86_000798"
     CDS             41014..41637
                     /locus_tag="SCJ86_000798"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1-acyl-sn-glycerol-3-phosphate acyltransferase"
                     /protein_id="HEG1168380.1"
                     /translation="MFYRFMRGVVRVLLALVNGNARYENKEVLPQDENYILVAPHRTW
                     WDPLYLAVAAAPKEFSFMAKEELFKNPVLRFILTRSNAFPVKRENPGPSVIKTPVKIL
                     KSTNLSLIMFPSGTRHATELKGGMALISRMSKAKIVPAVYQGPLTLGDLFKRQRVTVR
                     FGEPIDLSDIKKTDKEGMAEIERRTQAAFDKLDKEVNPDFKYEVKKK"
     gene            41829..42950
                     /locus_tag="SCJ86_000799"
     CDS             41829..42950
                     /locus_tag="SCJ86_000799"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628777.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exonuclease SbcCD subunit D"
                     /protein_id="HEG1168381.1"
                     /translation="MRFLHTADWHIGKKLQGYDLIEDQRHVLSSILDIAKEEKVEAIV
                     IAGDLYDRSVPAVDAVELYNELMVDWNLKEKIPIFAISGNHDSSTRLSAGTPWFSYSN
                     YFLRTQLAEAFEPVEFKNVQFFFLPYFEPIAARLYFEDDNIRTIKQAVAKVIDKMQEL
                     FDSEKKQVLISHFFVMGSVKSDSETKIEVGGLDSIPGELLTPFDYVALGHLHNQAALR
                     QDNARYSGSPLKFSLSEINQKKGVWIVDLTTGLELNFREIEPLHDIEEITASFKELLD
                     PAFYQSVNRENYIYIYLTDRTIIPNMMNQLRQIYPRILGIERANGRAEQTKRKRKVLE
                     MKDPQKLAEAFFSEMTEEQLTANQEKWLGETLQEIMEME"
     gene            42957..46085
                     /locus_tag="SCJ86_000800"
     CDS             42957..46085
                     /locus_tag="SCJ86_000800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327291.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SMC family ATPase"
                     /protein_id="HEG1168382.1"
                     /translation="MKPKKLQLKNFGPFINETVDFSRLTEAPLFLISGKTGAGKTTIF
                     DGITFALFGETSGRLRSGKEMRSLFATPDEETSVTFSFEHQQMSYEIERKPEQVLAKR
                     KGNGTKKQAAKVTLTIFDGKGKEIKQITKRTEADQLIRELMNLDAKQFSQIVLLPQGE
                     FRNFLVSSSSDKETVLRHLFGTQFFQQFNERLKEKAKQQQQKLDHLEQELHLLQKRFV
                     LVKEEEPAAGFEAVLAQWTNHQAILEKQITEEKEALTELQEKQKQLENSYYRFESLKK
                     SYEEKTILINKQKELAAQKDEIEEKKRWIGYYEFTQRLVEPIQRLQEIKVEKIELSQK
                     SSEQTKKLAMTTQNYEEWQKNEANRVRRQTKIKEAAQKLQNKQMMVPIVESLRSKKQD
                     AEQLTISLDKEELNAEKLYQNLAIYQERTRILQENLLDQEKLQEDRLAFGQLKYLDEK
                     QQEIEKQKAKDTGSLAQIREELKKATEEITKNSQLLKEQTEQAEQLKSQWAKQQIARL
                     QLFLRPGEPCLVCGSTEHPIQQLKHEQPNKEDILKVEQQLAEAESAVERTQQIIAKSE
                     AHENNLQQQLSDLEQKIMATNIQLAEQEEHLQQALKKAFPEITEQKTKTTLVVIEQSL
                     DRRKSKMANERIELEEIRQQAEKLEQQLAVYKQQQTEKNNQLQQLQGEIKSLEQQLQD
                     TDSNCLEEEIRRLEKQLEEDSNQLAVEQKAGVQLLQDLHTLRTQLDLQHEQIEKIDEK
                     IEKEQERIAKKLESQSYFSTIEEICAFAQQQAQYKENQRTVTSYHEEQLVTADRLRQL
                     ENIQLTETFPDTEEISQQLEEIRRMIEIKQEKIYGLQEQKISNQKIYDECLSIYHTSQ
                     TKLDELTQLQQLSQTFNGENPKKTSLERYVLQVYLQEVLQVANDHLQRLTKNRYQFEL
                     ADTIGSYRGKTGLEINIYDDQAGMSRSAHTLSGGESFIAALSLALALADVIQTQAGGV
                     AIDALFIDEGFGSLDEEALEMAMEALETIESEGRMIGIISHVRELKERVLQQIRIDTE
                     GSGQSKIRYQLG"
     gene            46104..49298
                     /locus_tag="SCJ86_000801"
     CDS             46104..49298
                     /locus_tag="SCJ86_000801"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SNF2 helicase associated domain-containing
                     protein"
                     /protein_id="HEG1168383.1"
                     /translation="MKWSIPEKVIARGRQYLQEGRVLSVVPDQEENVWHAEVLGSELY
                     MVDLDGTAKEIDYCQCPYWEEHKYCKHTVAVELYLRSKNLSRIMKKDQPMLEPVKATS
                     EGEIIAKGFSRLKTQNNPQAVQPLIIEYQVETIETNQYHPELSILALYLRIGSLASPK
                     KTYIVKNIYDFLQAYTEKETYTVNKQYQFRLDKRAFQKEDQKILTHLAGSAQTHQLLG
                     TNGLQVKGKLDKRYLLLPVEQARFLLEQMNQTTRLHLQINEQNDQGIQFSDQNKPLSF
                     KVEKFGEDYLLQALNDFDFFFMHYQWGVIQGTFYSLTKYQQTVYQTWKQLIRRLEKPE
                     VVFKKKELPSLFKEIIPLLSEIGDVAIAPAVEEEVADIPVEFIFFFRKAKGKIQARVD
                     FVYGEVIYSTDPKHEVSTEQAFQVLRDTVEEKRVLDLFRMYRYQKNETGYERPLPSGE
                     ELYAFFRTELSVFRQFGEVRLGRKLRELYLDAHKHQPKIEVTETGSWLDIRFDVTGIE
                     EQEIDHVLQSLLRNDAFYTLENGQVLSFDSEEFQQTSQVLQQLRESIRTEEGTIHVPK
                     NQGLIIQNQLEKSNATFSESFQTMVQDLIHPERYQAQLPKGLNATMRDYQKQGFRWLK
                     MLGHYQFGGILADEMGLGKTLQTIAFLLSEKEERKSFSALIVAPASLIYNWQAEVRKF
                     APSLSIQVINGNKKEREELLAKDTDIRVTSYASLRQDLANYQSQKIDYLILDEAQMVK
                     NSSTKTAQALRELAVPQRFALSGTPIENNLEELWSLFATIMPGFFPTKTKFRELAAEE
                     IAQMIRPFILRRDKQTVLKDLPEKTEMNLYSALTEEQKTVYLAYLRQMREEITSMDSE
                     AFKKNRIGILAGLTRLRQICCDPRLFIEDYQGGSGKLEQVKDLLVAAKENKRRVLLFS
                     QFTGMLTILQEELAELGISTFYLRGSTKPQDRLSMVDAFNAGEKDVFLISLKAGGTGL
                     NLTGADTVILYDLWWNPAIEEQAAGRAHRIGQKNVVEVWRMISEGTIEERMDSLQQEK
                     RELFQKVIQGNEEQLTKLTEEDIRMILSVGEIEE"
     gene            complement(49444..50151)
                     /locus_tag="SCJ86_000802"
     CDS             complement(49444..50151)
                     /locus_tag="SCJ86_000802"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HEG1168384.1"
                     /translation="MIKSVVFDVDDTMYDQQQPFRNAVKRVVPLVSDADMHPLYIRFR
                     HHSDENFPKVMAGDWTLEYMRAHRISQSLKDLDYPHITEEDGLLFQKIYEEELDNICL
                     HEEVKKTLDFLKEKNVPLGIITNGPTDHQTKKLKQLQLNNWIPSRNMLISQATGFQKP
                     EKEIFQLAEKEFHMLPEETLYVGDNYDNDVLGAKSANWQALWFNHRERKIEQTPICDI
                     EISSFDQLLETMKTIFV"
BASE COUNT        16456 a         8854 c        10507 g        14592 t
ORIGIN      
        1 aaggatgcta tcagcattta caaatttttt atttttattc ctaatcgtca attgaaagct
       61 aatatattta accatttgaa aaagatgcta aatgtgtctg ttactgaatc ggcacctgta
      121 aatattgaaa tcgtacctca aaatgtatct aaaggtgtgg tgtttgatcg tttggaatca
      181 atctacaatt taaagaaacc tttgagaatc gcgatcggag atagtttaaa tgacttatct
      241 atgtttgaaa gtgcagatat tagttttgca atgggaaata gtcatcaaga aatcatgaaa
      301 aaagctgatt atgtcactgt ttcaaatgat aaagacggag tatctcaagc atttgaaaga
      361 attcttacca tataaactaa ttaaggtagt atagaacata gaattctaaa tacggaaaaa
      421 tggagcataa attccagtac aaatagtact tcttcagtgt caaacgaagc gaatataaca
      481 tagatattta ggattgtgac aaaaatcatg tcacaatctt tttttcgaat aaacttattt
      541 tttgaagtcg gccatttatc gtaatttctt tgcataatac gaacaaattt tcggttattg
      601 atgaaaaaaa acattgaaaa tggtatgata aaagaaaaaa aggaggaaat ttttaatggt
      661 gaaatttatc catgcggcag atttacatat ggatcggtca tttgaaggac tgacaactct
      721 tgataaaact gtacaggaaa aactattgaa gacgaatctg accgttttat caaatatcat
      781 tgaacaggca ataataaata acgttgattt tgttttactt gcaggagata attttcatca
      841 aaatcgtcct tcattaaaaa tacagaaaca tttttcagaa cagatgaaac ggttagaaaa
      901 gaaccatata cctgtattta taatattcgg aaatcatgat ttctatcaaa aagagcggta
      961 ttggttctcc tttcctaaaa atgtccattt atttacatcc gaaacggtag agacaaaaaa
     1021 aataacgata aaaagtggag aaactgtctc tctcagtgga tttagttatc gtcaaccgtg
     1081 gattcaaaaa gacaaagtga tggaatttcc ttctagagaa ctcacagatt accatattgg
     1141 gttgtaccac ggggaacctg gcgtctctca aaaaggaaac tatgctcctt tccaaccctc
     1201 aaagatgcaa gaaaagggat acgattattg ggctttaggg catatccatg tgccaacggt
     1261 cttaaatgaa aagcaaacaa ttgtttatcc aggtgcgcct caaggtcata ctaaaaagga
     1321 acaagcttct acatctattc tactagttga attaagtaaa ggaagttgcc aaatacatcc
     1381 gattaaagta gcagaggtct attggaagac aaaggaaata tcattgagga aagcaagaac
     1441 gacaaaagag gtcattgttc acattcgtca gcagctttca aaagtagagg atggatttag
     1501 tttaattgag ataaaattaa aagattatga ccatctcaat acagatgttt tggaacggat
     1561 acaatcagga gagctattag actatttgtt agaggaattc tcagatagga taaatgattt
     1621 ctttatttgg cgtatttctc tcatagagaa tactttttat acgaagaccc cacttgctgc
     1681 ttctgctaaa ttgatggaac agttatttca atactatcag acacctcaag atttccaaca
     1741 aattttaaat gaagtatata gtcatcaaga agcggctaga atattgagcg aactgccgga
     1801 atatcaagaa gaaacacttg aaaaagccaa gcagctgctt aatcaagatt ttttatttga
     1861 ggaggatcag gaatgaagat tttaaggatc gagattgctg cttttggaaa atggcgtcaa
     1921 aaaagttttg acttttattc aggcaatcag ctgatctatg ggggaaacga agcaggaaaa
     1981 tcaacgattt atcaatttat tcaagcaata ttatttggtt tcccttcaaa aggccgtaaa
     2041 aaaaaggatt atacaccaaa agacggctca gcctatggcg gcaaaatatg gcttaaacat
     2101 cctgtatatg gtgaatttgc tgtcgagcgc tacaagcagc aaaatcgcgg aaaatcgaag
     2161 gtgtggctgg gggatcaagt gggaagtgat gagttactag aaaaactgat cgcacctttg
     2221 acgaaagagt tattccagca ggtatttact ttccagcaag agcagttgat ggaactagat
     2281 catttgagag aaaaagagct acatgatgcg ctgatttctt taggcatcac gggcagttct
     2341 caagtattcc aaaagcggca agaatattat cagaaagcac aacaattatt caagaaaaaa
     2401 ggccaaaaat tactaatcaa tcaaaaatta aatgaatgga aagagctgca agaaaaaatt
     2461 catcaaaaac aagaacagga agcttatttt ggcaagttga tccaacaaga acaggaatac
     2521 gagtcgcaac agcagcagtt attggcacaa ttaaaagagg cgaacaaaca attttcatta
     2581 gccagacagc aagaacttaa tttttcttta tatgaagaat ggcaatctct caaaaaaaac
     2641 agcactacag gtcttcctga tgagcaactt cttttagatc tggagtcttt ctccaaacgg
     2701 tatcagcaaa caaatgagcg tttggaagaa ttagaagcaa cactggaaaa gcatagcggg
     2761 atggatcagc agtcagccag atattatttt tatttagagc aggagcagga actaaaagat
     2821 ctccagcaac ttcaatttac agcggaacaa ttaaacgaag aatggcaaag attacaaatt
     2881 gagactagag aaacaaattc agaattgaag attttggaac aaaggtggca ttggcagaaa
     2941 aatccgttac cggaagaatt tttatatgaa gaagaatgga agcatcttct ggctgaagaa
     3001 agaaggattg ctgatctaga aagccagatt tctgtgcgtc tccaaatagc taaagaacag
     3061 caagagatga ttgaagcaga agtgaatgac ttggaagaga cctatccagc attattacaa
     3121 aatcagtcag agatagcacc agtctcttca tctaaaccgt atatgctgat ggctggcgga
     3181 gcaataatca ttgcaggtat atttgtacca atgcccttga aagtgatcct tttactaatt
     3241 ggtgcaggca tgattggcta tttcttctac aaaaggcaac caaaaattaa aactaaaaat
     3301 gatttctctg agataaaagg aatgtggcaa gaaaaactag gacaacttga tctagcctta
     3361 gctcaattag ctgaagcaga agaaaaacaa aagagaactg gagaagaaaa acagattctt
     3421 cagcaagaag tatatcgttt tgcggatcat cggcacttag gaaagatgga tacgctcaaa
     3481 ctcatgaaag aatatggaca ggaagtcata gattatcaga aattatatgg cgtatttgag
     3541 cggaaacata aaagagaaca ggaaatacaa gaaaaactac gacaaatcga ccaaaaattt
     3601 gagtttgtta aagaatggtt accgatacaa gataagacaa tttcagaaaa aatggtattg
     3661 ctttcccgtt ttatacaaga gatggaacag atgaaatttg ccagaagcta tcagcaaaat
     3721 actttattga agcaagaaat caatcaattg aaaaagaaac aaaaagtatg gatacaagaa
     3781 tacagcgaac aattgactca agcaggctta tcttatcctt cggaagtacc tgtcttttta
     3841 caaaaagcca aacaagccga acagcagatg aaaagataca atgaattatc ggaaatgctt
     3901 actccactat ttcctgaaga gctgacaaaa gagaaattat ctgtaagact gcgccaactg
     3961 aaagaaaagc aatcccaact ccagcaagaa gaaaaagaag caagtgaaaa tcggcaacga
     4021 ctccaacttc aagtagaaga gttgcaaaga gacggcacat tggacgaact ttatcaacaa
     4081 gctagccggc aaaaagcaaa attacaggaa ttactggagg aatggggagc acttgaaatc
     4141 gccgggacat tcttaggtga tctttcaaca gagatgagtg aaagacaatt accccagttg
     4201 ttaagagcag caggtgatta tttaggtatt ttaacaaaag gaaattatca gaagatatta
     4261 ctgaaaaatg atacactgac tttaacagac ggatatacag aatttcccat ctatgagtta
     4321 tctacaggta cgaaagatca gctgattatg gcgatgagat ttgcttacct ctcattagaa
     4381 gctaaccgtt cgatatgccc gatcatcatt gatgatggct ggcttcatta tgatcatcag
     4441 cgaaagtatc aattagctga acttttagct aaatttggag aaaagtatca aataatttgt
     4501 ttttcttctg ataaagaaat ggtaagctat taccatgaga tgaaccaaat cgtaaaacaa
     4561 ttagaaggag caaataatga aaaaaattcg tgatttagta atcgatgagc agtttgaaca
     4621 atttgtcctg atcaaaagtg cagatgtaag aatcgctaaa aacggaaaaa aatttattgc
     4681 ttttactttc caagatactt caggtactat cgatggtaaa tactgggatg cttcggagga
     4741 ggaaatcaat aaatttacag ccggcacagt cgtttttcta aacggtaaaa gagaggttta
     4801 tcaaggaaac cctcaagtaa agattcttca tatgcgctta acaagggtag atgagccagg
     4861 agagcctagt ttatacatgg aaagagcccc tttgaagaaa gaagaaatgg aagaagaaat
     4921 caatcaattg ttgtttgaga tcaccaatgc gcattggaat cgtattgttc gctatctact
     4981 taataaatat caaaaacaat tttttgagtt tccggcagca aaaagaaacc atcatgcatt
     5041 tgccagtgga ttagcttatc atactttgac aatgcttcac ttagggaaat caatcgccga
     5101 tgaatatcct gagctgaatc gagcattgtt gtatgctggt atcattttgc atgatttagg
     5161 aaaggtgatt gaactttccg gtcctatgtc gacagaatat acgcttgcag gaaatttgat
     5221 cggtcatctg gtattgattg acgaagaaat aactaaagcc tgtcttgcgt tgaaaatcag
     5281 cgaagctgac gaagatgttc ttgtattacg tcatatggtt ttagcacacc atggcttatt
     5341 agaatatggc tcacctgtac gtccaagaat aatggaagca gaaatattac atcaaatcga
     5401 caatatcgat gcttcgatgc agatgatgtt gacttcgatt cgtcaaacag aaccaggaaa
     5461 atacaccgat cggattttcg gtatggacaa ccgaagtttc tatgtaccaa aagatttata
     5521 gcaaaaattt atttttttca ttcaaaacaa aaaaagcgag acataagtct tcgctttttt
     5581 tgttttcttt tattttgatg aatcagttgt ttcttcagtt gaagattctt ttgttgaatc
     5641 agttgatttt gtgtcagaac tctttgttga agctgtagtt tcacttgagt ctttcgtact
     5701 tgatgaagtt gttgtgaatg cactcaacac attttcaaag gcatcatctt tgatttttac
     5761 atttgcatct tttaattctt caccgattac tttagttgtg aatgcattgt cacttagttt
     5821 agtttcagta gcgatatctt ttagttgatc tttgtattta tccatgtcat tgcctttatt
     5881 ttggtttttc accattttga caacgtagta ttctgtcgca taagaagtag gatttgttgt
     5941 agtaataaca tcggagatct caccgtcttt caatttgaaa gctgcttctt ttacttctgc
     6001 aggaatagtt gttgtcgttg agtcgaattt aactttaccg ccatcttctt ttgtctctgt
     6061 atcagttgat ttgtctttgg ctaatttaga gaaatcatct ccatcatccg ctgatttttt
     6121 cacatctttt gcttcatctt cacttgataa tttgatgatt tgtgcttcta cttctggatg
     6181 gaaagatttc caagcagttt tcaaatcgtc atctgtaata tctacgtgag ctttcaagcc
     6241 agcttcaaaa gctaaattgt tacgaatata ttctttataa gtatctttcg tatatccagc
     6301 agcttctaat tgtgattcaa aagtatcacc taatgatttc gcttgtttgt cgtattctgc
     6361 atccacttgc ttatcggtta ctttatcgcc atatttgtta ttgaatactt tataaatgat
     6421 catccgttgt actaaggatt ggttcgatga ttcaagtttt gcttcatcat agaaatcaga
     6481 tacagtgatc gttccgcctt tcatcgttgc gatatcttta ttagtatcgc ctgaacaagc
     6541 tgctagagtt aaaacagcca acgcactagt agctgccaaa ataattgatt tttttttcat
     6601 taaaatttgc actcctgtaa ttagttatta tttttatata ttaactatac catatcccat
     6661 ttcctttaaa aacgttgtcg tggtagtttc aaacaaaatt caaaatttca tcacattcct
     6721 aggaaatacg caaacttttc tcacttatat agttaaatct gttgagaaaa taatcattat
     6781 tatatttgtt caggatactc tggcagatca gcttttagtt tttctacttg ttcattgatt
     6841 tgtgtgatac gtggttcggc ttggaattta aaatcttcga tatctttttt gattgcttct
     6901 tggaaagcag gaatcacaga ttcagctgtt tcttttgttt cgatcaatgc gcttttaaaa
     6961 tgattcaggc tgttatttaa atcatttgtc agagttgttg cttcttccaa ttcttcgatc
     7021 agtttctttc ttgtttcatt cccgctcctg ggcgccaata acagacctcc gacagtgcca
     7081 gcagcagctc caaataacag tcctttgaaa aagtttttaa tcatcgcagg acacctgctt
     7141 tacgattgtt tcttgaatag cttttagttc ttccgatcca taactatctt gatgattgac
     7201 aaagtgaata gaaaaatcat cctctttgct gtagcgaggg actaaatgga tatgtgaatg
     7261 aaaaacagat tgataggcta gttcacggtt gttattcaag atattaagac ctttcatttc
     7321 tggaaaagct ttttccaatg cttgagcaat cttaggcaca cgcgaaaaaa cctctcctgc
     7381 aagttcggga tcatattcaa aaatatctgc tacatgacgt ttaggcacaa ctaatgtgtg
     7441 tcctttcgtt gcttgagaga tgtctaaaaa agcatagacc ttgtcgtctt catatatttt
     7501 atagcttggg atttcttgat tgatgatttt gcaaaaaata cagttttcca tataaaacat
     7561 cctttctata aagaataaat acttcagctt aactttacca taagttaaag ctattgtaag
     7621 gaaaaaaaca aaataccgtc attttcttcg tggtataata aataaaaata aatggcggag
     7681 gaaaaagaat gagtttaatt atcgatcatg tcacaggggg gtacggacat ctccctgtgt
     7741 taaaagatat cagtttcgat gtacagcctg gagagatagt agggttgatt ggactgaacg
     7801 gagcagggaa aagtacgacc atcaagcaca ttatcggtct gctggaagct tcaaaaggaa
     7861 cgatagaaat caatcatcta tccttaaaga aaaatacgga agaatatcgc aaacaaattg
     7921 ggtatattcc agagactcct tctttatatg aagaattgac attgagagaa catatcgaag
     7981 tcactgcgat ggcctacgat atacctatgg atatagcaat gaaaagagca gaaaagttgt
     8041 tggagacttt tcggttatct aatcgtcttg actggttccc agctaatttt tcaaagggaa
     8101 tgaaacagaa agtgatgatc ttatgcgcct ttttgatcga gccttcttta tatatcatcg
     8161 acgaaccatt tttaggcttg gatccactag ctatcaatgc attgcttgaa ttgatggtag
     8221 aaatgaaaaa agaaggagcc ggtattttga tgtctacgca tattttagct actgcagaaa
     8281 aatattgtga tcgttttgtg gtcttacata acggagagat acgagcagac ggttctcttg
     8341 agatgttgag aaaagaattt cagctgccgg attcttcttt ggacgaaatc tatattgctt
     8401 taacaaagga gcaggcacat gagtagtttt tttcaaaaac gtttagcaag acatcaaaag
     8461 aaaatgatga aatatatgcg ttatgtattg aacgatcatt ttgttttagt ttgtcttttc
     8521 ttgattggcg gtgtgggatt atattattct aattggttga aaaccctgca gccaccgttt
     8581 gcaattggag ggctcataat ctctgtattt tggatgctct gtttattcgt gggtaagatg
     8641 gctacttttg cagaggcagc agatattgtt tttttacttc ctaaagaaaa agaaatgcaa
     8701 acctatttgg aacgagcttt tcgctattct tgcatttttc cgttgatctt tttgatattg
     8761 gcatgcggat tggccatgcc attagtggtg gttagtacgg agcaatcctt ttcgatgttt
     8821 ttcgtctatc tgatcatatt atggagtttg aaattcagtc atctgcaaat caagagagca
     8881 gaattgtttc gactagatca acaagttatt cgttattgga aattaggctg gtttttttct
     8941 tctctgatct ttttgtttct atcattttat tggctaatat gggcaggaat agcaggtgca
     9001 ttagtacagg caagtctata ttactggttt ttgtggaagc aacaagaaaa aggattggat
     9061 tgggagaaaa tggtgcatgt agaagaacat aggttgcatc agatctatca atttatcaat
     9121 ttattcacag atgttcctga aattacggca aagatcaaaa gaagacgcta tttggatcct
     9181 atattgactc atattaaaag agaatcaaaa aacacttatc tttacttgtt tgcaagaaga
     9241 ttggcaagag gttctgattt tagtggtctt tatttccgat tagtaattat aggtgggtta
     9301 ttgattgccg gagtagtcga cttttatttt agtctgatta tcggtttctt atttttatat
     9361 ttgatcggtt tccaactgtt acctttagcg aaccagttcc gctatatggt tctcgttcag
     9421 ctttatccaa tccataagaa tcagaagaag caggcgattc agtatctact cacatgggta
     9481 ttaattatcg cgtcggttat ttttggtttg attgctgcag tcgtattaga tgggcagcag
     9541 cgatgggttc cgctagcggc ttatttgatt gttactatgc tctttgttaa actatatgta
     9601 ccaactcgtt tgaagaagat gtcagactga aaacaaagct gtgtagggta aaaatgagaa
     9661 tgaaaaatag agcgttgaca gtaagaatgc tgttcgttct attttttttg agaaaagctg
     9721 gttttttgaa tggcaattaa atgaaaaaga acgtatatgt gcttgacgtg tagtgttcag
     9781 gcgactagaa taataagtac tgcaaaacaa agagtaagag gacgggagaa tgcatgatgg
     9841 agtttcagtt agaccaagag tggcggttac gtcccatcaa aggcgacaca ggaaaaacat
     9901 acataggcat gcgtgatgag gacaaagtct ttattaaacg aaacacgact ccgatgttgg
     9961 ctgctctttc aagggaagga attacaccaa aacttgtttg gacaaaacga acggggaatg
    10021 gcgatacttt gacagcacaa gagtggctgg aaggtcgctt gctgactcct aatgagattg
    10081 gtcaaagaaa cgacgtgatt gatgttttgt accatttaca tcattcaaat tctcttaaaa
    10141 ccatgctgac ccgtatcggt ggtcgtgtca tgacgccgga gaagatgctg caaaaatatg
    10201 aaaaagaatt accggaagaa ctccagaaaa atcaatttct gcaaattgtc catcgttttc
    10261 ttcgtgagca gatcccgtca atagataagg aaaacttatc agtagtacac ggagatgtaa
    10321 accatcgtaa ttggttagta tgtaaaaatt atttatatct agtcgattgg gattcaatca
    10381 tgtttgcaga ccctgcggtg gatattgcaa cgattttagg aaattatgtg ccactgtcta
    10441 gctggaatca gtggctgatc agttatggca tacgcccgac tgacgaaatg cttgaaaaat
    10501 ttcattggta tgcggccatg aatttgctcc aagaaatcac cagatattgc aaacgtgaag
    10561 atcatcgacg gatgaacgaa gaaattttac aattaaaacg aattttcagt ggctgaggtt
    10621 tggcccaaac tctgcggaga tttggccaga cctttttaat tagaggaaag gaatggagat
    10681 aaataatgcg tgtccgaaat cgtccgggag cagcggaaat gttagcggct cacccgaatt
    10741 ttgtaattag tgatccaaca ttgtggaagg gaaaatggaa tgaattgttt gaaaacgatc
    10801 atccaatcca cattgagatt ggtatgggga aaggccagtt catcactgga atggcaaaag
    10861 cccatcctga aatcaattat attggagtag aaatgcaggt cagtgttgtt tcgattgcac
    10921 tagacaagtt gatcgaacaa ccgcttccta acctgaaact tctacatgtg gatggatcag
    10981 cgttgacaga gtatttcgct gacagcgaag tagatcaaat ctatctgaat ttttccgatc
    11041 catggccaaa aaaacgtcat gaaaaacggc gattgacata taagactttt cttgctgtag
    11101 atgaacagat tcttcgtcca aatggagaaa tccatttcaa aacagataat caaggattgt
    11161 ttgagtattc tctggcaagt ttttctcaat atggcatgat cctcaaacag gtatggttag
    11221 atttacatca aagtcaattt gaaggcaata ttatgacgga gtatgaagaa aaattttctt
    11281 caaaaggtca acgtatttat cgagtagaag ctcaatttca agataagacc aaaaagaaaa
    11341 gtctgtgaaa ctaactttct gctttcataa aaacattaat atccataaat aaatctcaga
    11401 aaatgaacat tacagatgta atgtattttc tgggatttat ttgatttaaa atgtatttat
    11461 ttcatcggtt tttccacaag gtggaatcaa ttggaagcga gaaaatgaaa ataatccttt
    11521 gtcggtgatc ttcaattcgg gtatgacaga taatgttaag aaagaaagtg tcaaaaaagg
    11581 atcaaaggtt ttagctgctc ctagttgatg tgctttagtt attaattcat tcaactgttc
    11641 atagacgatc gaaaaaggtt cctgtgtcat gattccgcct ataggaagag aaacacaagc
    11701 tatttcctgc tgtttgttga caacggtcat cccgccacct ttttgaatca aaagattagc
    11761 agcatacagc atatcctcgt cgtttgtccc aacgacaact agattatgcg agtcatgggc
    11821 aatcgttgtc gctatcgctc cttgtttcag ctgaaatcct ttcactatgc ctaatccaat
    11881 gtttcccgtt tgatggtgac gctcgatcac cgcaatcttt aattggtctt tttcgattga
    11941 aggaatgaat cgttcgcctt gtcggtccac tttttctacc aaatctttag tcactagact
    12001 attcgggata atttcgatca catgggcttg tgaagaagaa agaagcagct ctaacgaatg
    12061 tggatttaat ggttgagcgt ttagttttgg cagacaacac gtatatgaat ttgaatgatc
    12121 agcaaataaa tggtcttgga gttgcccaga ttgaacaact aatgttccct tatagaaaac
    12181 ttcatgaatt ggcagcttgg taagatcgtc tgttaagaag aaatcagctt gatacccagg
    12241 ggcaatcgca cctagtgttt tcaaatcaaa acattctgct gcattcaatg ttgccatctg
    12301 aatggcttgt aatggatcga ttccaagtgc aatagcttga cggacgatat gatcgattga
    12361 tccttctttt actaaatcat tgatcagttt atcatcggtg acaaagaagc atcttcttga
    12421 attttcaggt gtgatcgccg gtagtagcga tgccagatct tttgcaacgg tcccttctcg
    12481 aaccattaaa tacataccaa gatccaaacg ttctttagct tcttttgcag ttgtagcttc
    12541 atgatccgtc cgaatacctg ctgcaggata aacatttaac tcttccatgc cgataccagc
    12601 tgcatgcccg tcaatttttt tcttatgctg gtgcatcaat tggatctttt ctaggatgtc
    12661 tgtttcatta tttgctactg cttgatagtt catgacttca gctaagccta aaacttcggg
    12721 tttttgtaaa aaaggggcta atttttttgc atttaaattt gcaccattgg tctcaaaagg
    12781 ggttacaggt acacaagaag gtagcataaa aaagatattc atgagtgttt gtcttgcatc
    12841 ttctatcata aactcaagac ctgtactccc agcaatgttt cctatttcat ggggatctgt
    12901 cactacagta gtaactccat gaagcaagga tacttttgaa aattcgcttg gtgataaaag
    12961 tgagctttcg atatgcacat ggccatcgat gaatcctggc actagatatt gcccattagc
    13021 attatgtacg ctttttcctt cgtattcacc gattcctacg atcattcctt cgcagattgc
    13081 tacatctttt tccatgattt ctttcgtaaa gacgttgacg atcttagcat gcttgatcac
    13141 taggtcagca ggcttttttt tacttgctac ttcgatatat ttccttaatg ttgtttccaa
    13201 tggagtgcct cccaagataa atttttataa gtataacata aattcggctt ttgccaatat
    13261 ttttttgtaa attcatttta tttgaaacaa aaagatcaaa aagacgtatg ttttttgtgg
    13321 aggggagaat aatggaaata aaacgattat cggatcaaat gataagtgaa agttttgcca
    13381 aacatgccca agacgtatat atattgccag cttcagaagt attttacatc tattttcgct
    13441 atggaaaaaa acgaacattc tggaagaaag tctcttcggc agatggacag caactgatct
    13501 caagatttaa gtatttggga cagatggata tcggagaaaa aagaaaagct cagttaggtg
    13561 catttactta ttctttagaa aaacaggaag ttcgtctgcg gttgtcttct gtaggagatt
    13621 accttcaacg agagagttta gtgattcggc tattatacgg tacttttgaa acaccccatc
    13681 gttgctttag ttctcgagat tttgcaacta ttttacagaa aaccgtcaga cgtggattat
    13741 accttttcag cgggacagtc ggatcaggca agacttctct aatgtatcgg ttggctttgg
    13801 aagaaaaatt gcaagtgatt actatcgaag atcctgtaga gatagaagaa ccgttttttc
    13861 tgcagctaca gatcaatccg aaaatagcac agacttatga tacacttttg aaactcgcat
    13921 tacggcatcg tccagacttg ctaatcattg gcgagataag agatgaatca actgcacatg
    13981 ctgcggtaag agcagcacta acagggcacc gggtttttgc aaccgtacat gcacgcaatc
    14041 ttgaaggtac attggtcaga atccatgaac tgacaaagaa aaaagaagag ctatctgaat
    14101 gtttagctgg tgtcatttat caagaacttt tggaagatgc caatcaccat ccttctgttt
    14161 tatggagtta tcaatttatg gaggaagaat cagtaaaaaa atcatgggag gaaagttatg
    14221 aagaagctca ggaaaatatt tggcagtcag aactattcta aaggattaac aagaagccag
    14281 caaaaccagt ttgcacaaac catgggagaa ctgcttttat ctggttttag tctgcaagaa
    14341 tccctttatt tattactcac gatccgttcg tttccacctg ttattttggc aaatatccaa
    14401 catcagctaa aggaagggca agcgtttagt gaggtacttt cgcaaatagg atatggagca
    14461 gagcaactgg tacagatcga gttagctgaa cgtcatggga atctgccgac aactttgttg
    14521 gaaattgcca aacagtttcg gcttgttgaa ggatttcgca atgatttgaa aaagctgctt
    14581 gcatatccga tgctattgct cacctttttg ctaggtatct tgtttagtat gcggttgttc
    14641 gttttgcctc agcttattgg ttcaggaatg gtcatgcaag agcattgggg tattcagctg
    14701 atacaagtct ttcattggta tgttctacta ttcatcctag cggttactct attggcttta
    14761 ctagtaaggt atcgtctgat gaagttgtct cctgttgatc agagtgaatg gataagcaga
    14821 caattattga ttggaccgat atattccttg tatcaatcgg cttatttgtc actagagatt
    14881 ggaaagcttt ttcatgaagg tttcgaatta aggcaaatca tagtctgctt aaaggaaacg
    14941 aaacaagaaa gtctgattca gcatcttgcc tctaagttga ttcaaggatt ggaggcaggt
    15001 atgtcattgg ctgagcagtt ccagaattat cgttttctat cagaagaatt cagtaaaatt
    15061 gttttacagg gagaggcaaa gggaagttta ggtaaagaac tgatttttta cagcgaatgg
    15121 acaagaaaac agcttttcaa acgtcttcat cactggatgc attttattca gccaatcatt
    15181 tttttgggcg tagcggcttt gattttatta gtttatgccg ctgttttatt acctgtttac
    15241 ggaagcatag aggaggtttt gccatgaaaa agagaaaaat atcttattat agcggtttta
    15301 cattgatcga aatgctaatc gtcttactga tcatcagtgt attagtgttg ttgttcgtcc
    15361 ctaatttgtc gcgttatagg aatcatgtag accaagaaag tcgtgaagcg atcatccagt
    15421 tggtagacac acaaaaagag ctctatgcgc ttcaaaataa tggaagagtt cctactgtgg
    15481 aggaattgtt gaatgaaggc tatatcaaac gagaacatgc agaaatctat cagcggcctt
    15541 gaaggtaagg ttttagcatc aggatatacg ttgtttgaat cactcttact gattttatta
    15601 cttacaacga tcctttcttt tccagttctc gcattttctg catggaagaa tgaattggca
    15661 gtctatcaat ttttcggcca atttgaaaaa cgaatctatt ctacgcaaaa aatcgctatt
    15721 gtgaatcaga tccaaacagg cttttattgg aatagtgaag agaatcaaat catttttcat
    15781 gttccgaatc cagataaaat aaattgggaa gtattagata ttcctgatga gattaccttg
    15841 aaacgccatg cttctattac ttttgctgct agaaccggga atgaaagttc actaaaagct
    15901 tatcaatttt attgggaaga aaaagagcgg acgattacgt atcaattcca gatagggagt
    15961 ggtcgctata caaaaagaat tgaataaaac aggatttgtt ttgttggagt cccttacagc
    16021 gcttatgatc gcttctgtct gtgtctactt agtgagcttt ggccagctgt ttttgcttca
    16081 ggcagaaaag aaaagtgggg aggaactgct atttattcga atgatttatg aagatatcaa
    16141 gacgcagcgg atgtatcaca aaagaccaga gcgaagggaa gaaatgatcc catacgatat
    16201 ccaaattgtc gataataatg gagtaatcga aaaagtgttt gtcaatatgg agaaagaagc
    16261 gatcagtatt gaaaaaacaa gtaattccag catttacgct gattgaatgt ctaattggac
    16321 tgatgatatt gagcagtcta ttgctcacat tcaatctttt gattcagcaa accatccaag
    16381 taagtgaatt tttgcagcgt aaagatcaaa aagagtggct aatattttta gcacaattag
    16441 aagaagaact caagagcagt cacaatgtct ctgtgaaaaa tcagcaactg cactacacga
    16501 tcgaggataa acagtatatc atcgaaagat atcaggcaat gatacgtaaa agaagaactt
    16561 ccggcggaca tcaacctatg ttaacaagta tcactgagct tcaattattg gaaaaagaac
    16621 acacaatttc tttttatgta cactttgaaa atggagaaga tggatatgcc atatggacaa
    16681 aaaacgatca gtagaaaatg ttataagggc tctattttat tttctgtctt gcttttattt
    16741 cttttatgta cgtttttatt gatggcagca ttagaaagct accgtttgtc aatggatctt
    16801 aatatacgaa tcaaaaatta ttatatagca aaaataatga agcagatggc tttatcggaa
    16861 cagtctacgc ttgccgaaag ttcggaagga gttttttact acacaactgg atctgtaaca
    16921 tatcagtggg tacaacagtc cctattctta gaggtggaag tctcaccgtt tatctttcgg
    16981 ttcacagaag aagtaaaaaa tgatacagat actagtacag aatgatggaa tgttcgtgaa
    17041 aacgccctct tttctgttat aacactgttt ttaataaaaa aatgtttgcc gtttcacaaa
    17101 taaaaaacag tgaaaacgat tgaaattttg tcaattgttc gatagaatta aacaggtgaa
    17161 aaaattgtta taatatatct agaaatgagg tgcaccatgt taccagaaaa aatggaacaa
    17221 gggtttaatc aaaacctgga agctattcag cttttacaga atgcattggg aacttccttt
    17281 ttagaagcct atgtcgaaaa tgcagaaaat ttaatagatg attatcaagt acgtgtggtg
    17341 gatggagtgc cgacaaaaga gacaacgcaa cggatcacag ctctttatga agagttgaag
    17401 aaactttctt tcgagccaga agaatggcgc agactgtctc aattactttt attgaagggc
    17461 agccagacag aacatttgca ggcgaaccat caactcactc ctgatagtat cggtttttta
    17521 tttgtttttc ttattgaaca actatacaca aataaaaaag caccggttaa gattctggat
    17581 attgcggcag gaatgggcaa cttgttatta acagttcttt taaacctatc gaatgcgggg
    17641 taccaaacag aaggaattgg tgtggatatt gatgatacat tgttggctgt cgcagcttct
    17701 acaagtgatt tgacacaagc aaatgttcaa tactttcatc aagacgggtt gcaggaatta
    17761 ttaatagatc ctgtcgattt tgcaatcagc gatttgccaa tcggttatta tccgaatgac
    17821 gaaaaagcaa aagagttttt aacaagtacc gaagaaggcc atagttatgc acatcattta
    17881 ttgctagagc aatcaatgaa atatgtgaag ccggatggtt ttggtttgtt tttgatgccg
    17941 agtggatttt tagaaacgga tcaaagtgaa gaaatcaaaa aatggtttaa agaagaaggt
    18001 tatcttcaag gaatgattca attgccagat gaattgttcc gtaataaaca atcgcaaaaa
    18061 agtattttga tcttacagaa aaaaggacca caagcacaac aagtaaaaga agtcttgctt
    18121 gtgaaacttg cttcattaaa agaaccagag aaggtgacgg aatttttcaa cgagtttaaa
    18181 aactggaaat catctgctct gtaatcattc aacgagaagg agagtcatat gtctaaaaca
    18241 attgcaatca atgctgggag ttcaagtttg aaatggcaat tatatcaaat gccagaagaa
    18301 accgtagttg cgaaagggat cgtcgaacgt atcggtttac aagattctat ttttaccatc
    18361 aagtacggcg ataatcaaaa attcgaacaa atcatggata tcgaaaacca cgaagtagca
    18421 gtaaaaatgc tgttagatca gttgattgaa ctgcaaattt taagctctta tgatgaaatc
    18481 accggagtag ggcaccgtgt tgttcacgga ggagaaactt atggggattc agtagtgatc
    18541 gatgagaaag taatggaccg cattgctgaa ttagcagaat ttgctccttt gcataatcct
    18601 gcaaacttaa tggggatcaa agcgtttaaa aagattttac ctgacatttt gagtgtggct
    18661 gtattcgata catctttcca ctcaacaatg ccaaagcaaa attatttata tagcttaccg
    18721 gtgtcttatt atgaagattt caaagtaaga aaatatggct tccatggcac aagtcatcgt
    18781 tatgtgtcgg aacgtgcggc aaaattattg aacaaaccga tcgaagaact aaaaatcatc
    18841 acttgccact taggaaatgg tgtatcgatc acagcagtcg atggaggtaa atccatggat
    18901 acttctatgg gattcacacc attagctggg gtgacaatgg gtacacgttc aggtgatatc
    18961 gatccagctg ttctcccata tttaatggaa aaattagaga ttgatattga tgaaatgatc
    19021 aatgttttga acaagaaatc tggtctgcta ggcctaacag gattgtctag tgatatgcgt
    19081 gatcttgaaa agaactatga aaaagaacat attcgtttag cttatgacat tttcgtggac
    19141 cgcatccgaa aatacatcgg cggttatgtg acaacgatga atggtgttga tgcaattgtc
    19201 tttactgctg gtatcggcga gaatgacgga catgtacgta gtgaaatcat caaaggtatg
    19261 tcttggtttg gttgtgaaat cgatccagcg ttgaatgaat tgcgtggtga agaagtagat
    19321 atctctacaa aagaatcaaa agtaagagta ttagtgatcc caacagatga ggaactaatg
    19381 attgcccgtg atgtggagcg tctgcgcggc taagccaaag cattcgttag gataaaagat
    19441 gaaacgcatg caagagggaa ttcttgcatg tgtttttttt gcggagaaga ccgcaaaatg
    19501 gtaatttaac ttcaatttcc cttctttttt acaggatctt cttagggaat tttgcttgga
    19561 aaagtggtca aaagggaaaa gactggcatt ttatttattg attttcagga aaatcccaat
    19621 attttatttt cattcatctt tataagggac attacatatg taaaaagaaa aaacattttt
    19681 agatcagcct cgtgggctgt cgactttatt cttcgcttta tgtttagcta gaaaaccgat
    19741 ccgaaacatt cattgatcga ataataaaaa cttgattttc cacctgtact ttgcctgaat
    19801 cgtgaaagca caggtatgaa aatcaagttt tttgttgcga aaaaacgtcc tagtcaatca
    19861 gttggccgag cggctggcgg ctgatctgct cctgatcaat aacgagtgct tcttggaagt
    19921 gttgctgcaa aaagtagcgg tcatggctga ctgctaggag cgttccttga tagtcttgca
    19981 gcatttcttc aatttcttct cgagcatagc tgtcgaggtg attggttggt tcatccaaaa
    20041 tcaggacgtt gattttttct aaaaagagtt tcagcaacgc caaacggatt ttttcaccac
    20101 cggataaatc tttgatccgt ttacccacat cgctttgata aaagccactt ttggctaatt
    20161 gccgtcgagc gacttcttcg tttccagtca atgtgatggc gtaagccagc aaacggctat
    20221 cgggatcggc aaactgcagc tgctgcggca aataaccgat ttgcaagcta ctacctaatt
    20281 tgatggtgcc accgtaaggc ttttcctcac cgagtaataa tttgagtaag gttgattttc
    20341 ctgcaccatt gtcgccgatg atggctaaat gatctccacg atagactgca aaactgctgt
    20401 ctttgaatag tacttgatca gcgtactgct ggtgcaagtt ttgaatgatg aaaacttctt
    20461 tgccagattt tccaccatta gacaagcttt gcagccgatt tttcggggga ggtggcggtt
    20521 tgacgagtgt cagtttcgcc aggcggcgtt ccagttcttt ggcttttttg aagaaggact
    20581 cattgtctcc ttcatgtgcc cactgacgaa aacgacgaat catgactttc aaccgctgga
    20641 cttctttttg ctgcagttcg taattttttg tcagctcagc caagcgagct tctttcaaga
    20701 ctcgaaaacg gctgtagttt ccgggatact caatcaatgc accttcttcc agttcccatg
    20761 tttttgtggt gacttgatcg agaaattgcc gatcatgaga aacgaccaga accgattttt
    20821 ttgtattctg taaatagttt tccaaccagc gaatactgtt aagatctaaa tgattggtcg
    20881 gttcatcgag taaaagtaaa tcggcatcgg ctgctaaaat tttggctaaa gcgactttaa
    20941 ctcgttcgcc tccgctaagt tctgttaacc gtgcttcctg acagtgtgga atccctaagc
    21001 cttgcaagat cgctgcaatc cggtcttcta atacgtaacc gcctagttct tcaaagcgat
    21061 gctgcagctc accgtaacgg gccagacact tctctaagtt tgtatctggt tctgtcatca
    21121 atgcttcgta gcgactcatt tgctgtttga ggtcctgtat ctcagcggca gaatggagca
    21181 agtagtggtt gacggtcgct tcggaaactg taagttcttg ttcgaccgtc ccgatcttta
    21241 agcctttttt gcgactgata acaccgctgt cgacaccttc tctgccggtc aagatttgta
    21301 aaaaggttga tttgccagtt ccgttgatat cgactaaggc aattttttca ccagcttcaa
    21361 tttgacagga taaatcctca aataatggat tcccgccgat tgttttttta atagaaattg
    21421 cttgtatgat catgatctct ctccttgaga ccactcaaaa aggccatggt tatccctggc
    21481 ctgtgttatt taaactcaga ttatcatgag aaaaatacct tgagatggcc attgatttta
    21541 cgtgaattca actgttttgg acaaacttgt cccgtcaaca gcaactgtaa aaccaattgc
    21601 atggctaaat gcgatatggt atcgttggta attttcatta cttgttattt tagtcatgcg
    21661 catcatagta ttttccctcc ttcgtgtagt tgtcttttcc agtgtacctg aaagtgtcag
    21721 aaagttcaac tagcgctgac atttacttaa tatttatcga tgtgtaattc tgtcagagag
    21781 ctttttttat ctgactgagg gtaatgtctc gtggaccaca tcattgtatt ctgagctaaa
    21841 ccagcgttca ggcgaaatca tatggtgttt gataaaaaac tttgtctaca tgacttcctt
    21901 ttgccatttg cttaataccg ttgcattaaa cagctcttct ctttggaaat ctcaccaaca
    21961 tcgcgttcag gagtatcgtt gtggttgcaa tggcaagcgg tagcctttca tcaattcttg
    22021 aatgatttac cgaatgtagc acttgccttc tcgcggtacc cgaacccgaa ataaaatttt
    22081 agtttggcga taaaatcggt atccgccatc gatacttcat attttttcgt atcagattca
    22141 tcggaaactt ccacattgac gaaccaataa gctttggcat gcttaggtcg ttttaaacca
    22201 caccagggcg actaatcgcc ctggtgttca ctttttttat tttacttgaa ctcattgact
    22261 gttttccctt tattttaaac attataaaga ccgatgagtg cttgccgcgt cttttgtttt
    22321 tatcacggtt ttttgtgcaa aaaaacctgc cgatagttgg caggtcgtct acgtgttaag
    22381 ctttggtgtg gtctttagct ggcaattggt tttctaaatc agttcggaca acaagcacat
    22441 cgcaagctgc gtttctaata acatattcag aaacagaacc aataaataaa cgttctacag
    22501 cattcaatcc tgtggctcct aacataatca aatctacttg attatcttct gggatttctc
    22561 tggcaataat ttgttttggt gagccatatt ctacgacaga ggttacatta ttcaagccag
    22621 ctttcttcgc attgctttca taatctgcca atgtttgctt tgccatttca gtagcttgtt
    22681 ctgcaagcat tccatcaaag gatgaaacgg tttggaaaga acgtgtgtca atgacatgtg
    22741 ctaatagaag ttctccgtta tttcggatag ctacattgac cgccttttta aaagctaatt
    22801 cagcttcatc agaaccatct acggcaacca tgattttttt gtattgttgt aacataagca
    22861 ttcactcctc aagtcaatcg tattgtactc ttttattgta gaacacttta gggaaaaaga
    22921 gaattattta ctgctttggt tcagcaattt tttttgcaaa tcggatactg aaggtggcag
    22981 aaacaataat tacgatcagt aaaaagacca aggcaataaa gcgttgattg aaaaaagctg
    23041 ttaaaatacc cattacgcct aaaacagcat atgcagcacc gtaaaattgt aggaatcctt
    23101 gatttttatc atttttgttg atcaatacaa aaaagatatc ctgtttatta aataaataat
    23161 agctaacgaa taataatact aaagtggcga taatcattaa aatcctaatc atgaacaaat
    23221 cctccaaaat aagcaacaaa tagaaaaagc aatttgccaa gagcaaattg ctttattctt
    23281 taaaagatgg taatctccgt taatgccgtt gtttgtcctg tagtattgat cccgcgaatt
    23341 caagcaggtg ggtcccatgg tcgtagcttc gaactaccaa atgataaggc atgttaccag
    23401 cagtgacaca agacgggtcc caagatatca ataaaaatgt tcggtcaaca cacaaagagg
    23461 acaattcgaa catcacagat ttcccacctc tatagtagca tatgaattta aaaagagcaa
    23521 tgagagagct cgcagaaaca gaaccgtttt cttgtctagt tttttggagg atgctttttc
    23581 cactctttta tacggtaata ctgttgaccc agagcctgtt catatttccc tgtatctttt
    23641 ggctgataat actgagcatt cttcagttta tctggtaaat attgctgatt gacccaagcc
    23701 tgatcaaaat gatgaggata ctgataaccc acgccacggt tgagtgattt cgcacctttg
    23761 taatggctat ctctcaaatg atcaggaaca tctccagctt tgccctctcg aatatcagca
    23821 agtgctgcat ctaatgccat gtaggcagaa ttggattttg gcgacaagca aagatcgacg
    23881 acgacgtctg ctagtgggat acgtgcctca ggcaatccca atttttcagc agctaataca
    23941 gcgttcactg tacgagccgc agcagcagga ttacctaaac ctatatcttc ataaccgata
    24001 accatcagac gccggcaaat acttgccaga tcacctgcct caactaaacg ggcgagataa
    24061 tgaagtgcag catccacatc acttccacga atcgattttt gaaaagccga aataacgtca
    24121 taatgagcat ctccattttt gtcatgggta agagcttttc tttggacaca ttcttcaatg
    24181 atcgacagtg taagatgaat ccttccttct ttatctgaag gagtcgatag tgtagccagt
    24241 tccagcccat tcaatgcact ccgcaaatcg ccgttcgttg cacgcgaaag gtgaagaaga
    24301 gcatcttcgt ctaactggat tgcttgttgt ccaaggccgc gttctttatc ccttaaagca
    24361 tgttcgactg ccaactggat gtcttgttct gttaatggtt tgacttcaaa gatctgtgta
    24421 cggctgcgga tcgctggatt gatggtaata taaggattct cagtagtcgc tccgatcaag
    24481 atgattcggc cactttctaa atgaggaagc agaaaatcct gttttgtttt atctaatcgg
    24541 tgtacttcat ccaataagag gatcactgta ccactcattt ttgcttcttc cgctacaact
    24601 tgtagatcct tttttgaatc tgtagcggcg tttagcatgc gaaatgcata attagtagag
    24661 ccggcgatcg cacttgcgat actcgttttt ccagtaccag gaggtccata tagaatcatg
    24721 gaagagagca tgcgagcatc gaccatgcgg cgtatgattt ttccttcccc gactagatgc
    24781 tgctgaccaa cgacttcatc gatcgttcga ggtctcatgc gataagccaa tggctgttgc
    24841 ataggatttt cctccttctt gtttttacgt cccttaatta tatcatattt agcaagatgc
    24901 ctagaaagaa cagccttctg ttgttttgtt ttcatagctt atccaactaa tattgtgtat
    24961 gataaagtag gtgagaaaaa tgacggataa acaaaagata gatgaatttt tttggaataa
    25021 aacgacgcca gaagtggcac gggatttatt ggggatgtat ttggagcatg acacaccaga
    25081 aggaagatta gctggatata ttgtagacgc ggaagcatac ctaggtccag aagacgaggc
    25141 cgcccatagc tatggtttaa gaagaacacc gcgtgtgcgg gcaatgtatg aaaaaccagg
    25201 tacgatctac ctttacacga tgcatactca tcggattttg aacatcatta ctcagccaga
    25261 agggattcct caaggagtaa tgatacgtgc aatcgaaccc gccgcaatga ttgatcaaat
    25321 gagtaaaaat cgtggcggaa aaactggtcc agatatcagc aacggtccgg gaaaattagt
    25381 ggaagcatta gcaattcctc aagaacttta tggtcagtct atagcagata gttcgttacg
    25441 cttagttttt gagaaaaaaa agacaccaaa aaagatcaat gctttgccta gaatcggtat
    25501 cccaaacaaa ggtgtatgga cagaaaagcc gttgcgtttt gttgtgtcgg gaaatcctta
    25561 tatctcttta caaagaaaaa accaaatcga gaaagattgg ggttggagga aagaaaatga
    25621 aaaagaagga tctattaaca tatttagatg aaataattga aaaacaagtg acagactatg
    25681 atgtagcttt agattgggat acaaaaaatc atacgatcga gatcgttgtt cgcttgtttg
    25741 cagaaaataa agcacaattt gagattgatg atgcagaagg gatcgtttcg caagaaccaa
    25801 ttatcgaatt tgaagacggc gtacttcttt ttaatcctca gaaatctgtt tttgatgaac
    25861 gcaattatct agcagtaatc ccttatgaag gaaaaaaagg gttggcaaaa tcagtagctg
    25921 attctttagt ggaatatcta aatgaagtat tagctcaagg gcaaagcgat ttgcttgatt
    25981 ttttggatga agacgatgac gaagctgtat tcgagcttca ctgggataat caacaactag
    26041 cagaattagt agaagtaaaa cagcaaaatg aagcagatac ttatttaccg tacccaagtt
    26101 attaaggagt gacagatatg aaatggaatg aagtaaaagt agaaactgaa agtgaagcag
    26161 tcgaagcagt tgctaatatt ttgatggagg caggggcgag tggtgtcgcg attgaagatt
    26221 ctttagatgt tgaacatttt cagtcggatc aatatggtga gattttagat aaaaatgatt
    26281 ttacatcgct gaaagaaggc gcactagtca tggcttattt tccggaaacg actttcttac
    26341 cggaaatctt gccttttatc aaagatcgta tcacacaatt acctgagttt ggtctagcta
    26401 ttggaaaaaa taaagtaaca gttagtgaag tagaggaaag caactgggca acagcctgga
    26461 agaaatacta tcatccggtg cgtattacgc gaatgctgac ggttgcgcca agctgggaaa
    26521 actaccagac agaagatcct ctagaaaaaa ttatcacact agatcctggt atggcatttg
    26581 gcacaggcac tcatcccacg acttccttga cattgacagc actagaaaca gtcttgcgtg
    26641 gtggtgaaac attgctagat gtgggaactg ggtcgggcgt gctaagtatt gcagcaaaat
    26701 atcttggtgc aaaagatgtc tatgcctttg atttagacga agtagccgtt cgttcggcga
    26761 aagaaaatat ggatatgaat gaaatagcca aagatgtcca tgtatcagct aacgatcttt
    26821 tgacaggaat cgagattgaa agtgatgtaa tcgttgcgaa cattttagca gatatcatct
    26881 tattgatgat cccagatgct tggcgtttac tgaagcaaac aggcacgctg atcgtttcag
    26941 gaatcattga agagaagaag caaatggttc tggatgccat gttagaacaa gggtttatcg
    27001 tggatcaaat tttccagcaa aaagactggt atgcaattat tttgaaaaaa ccagaggtgg
    27061 aatagatgca gcggtatttt ttaaaagaat catatgaatc aaaagactat tttgttttag
    27121 aaggggaaaa ctatcaccac attgtacggg ttatgaggat ggaacccgag gatgaagttt
    27181 ttttggcatt caatgatcaa atagccattg ttgcagaaat cacggagatt actgaagagg
    27241 ctgtttatct gaaagaaatt aaaaaagaag aaaatcaaaa ggaactgcca agttcgatta
    27301 ccatagcgag tggctatcct aaaggggaaa agctggattg gatcgttcag aaatccactg
    27361 aattaggagc gcaagctttt cttggtttcc cggcatcagc ctctgtcgtt aaatgggatg
    27421 caaaaaaacg tatgaaacga caacaacggc ttgaaaagat tgctcaagag gcagcggaac
    27481 aatcccatcg gcagatcgtg ccatcagtag agttattgga aacgaaacaa gatttatttg
    27541 agcggctaga tgattatgac gtggtcttgg ttgcttatga ggaatcagca aagcaaggcg
    27601 aaaaatcaaa tgcagccaaa attcttagtg aaacaaaacc agatagtcga atcttagctg
    27661 tctttggacc agaaggtgga ttatcccgcg atgaagtcga ttattttgta gaacacaaag
    27721 ggatactttg tggattagga ccaagaatcc ttcgtacaga aacagcacct ctttatttat
    27781 taagtgctgc tagctatcaa tgggaattaa tgaatccttc tttcagctaa cgttcttcat
    27841 taataaaaga gtatcgacag aaagaattga aaaaatagtc aacaatttgt ataatagaaa
    27901 caattattta tacaatggat acgaaaaatc gtttgaaaaa gcttttaaaa agcgaacatt
    27961 tccgtaaaaa aacgtataac tgcacggatt ttgggacaat tatcaaacag atatttgtcc
    28021 cgattcccgt tttaccgaaa aaagaaaggg ggatcgattc ctgtgacaaa agaagaaatt
    28081 ttaacgggac ccggtgtcat aaagctcgtt agtcaataca tgggtcccga acatgtagca
    28141 tttgttgaga aagcatgcga atacgcgact gctgcacatg acggacagtt tagaaaatca
    28201 ggcgaaccct acattattca tcctatccaa gtcgcaggta tattagcaga tttaaaaatg
    28261 gatccccata cagtggctac aggcttctta catgatgttg ttgaagatac agaaatcact
    28321 ttagaagatc tgagagaaga atttggcgat gacgttgcta tgttagtgga cggcgtaacc
    28381 aaattaggga aaataaaata taaatcccac gaagagcagc tagcagaaaa ccaccgaaag
    28441 atgctgctag caatggctca agatttacga gttatcatgg tcaaattagc tgatagacta
    28501 cataacatgc gtacgttgaa gcacctgcga gaagataagc aaagaaggat cgctcaggaa
    28561 actttagaaa tctatgcgcc tcttgcacat cgtctaggga tcagccggat caaatgggaa
    28621 ttagaagata cagcacttcg ttatctaaat ccaaaacagt attaccgtat cgtccacttg
    28681 atgcagacga agagagaaga acgtgaaaaa tacgtaagtg gtactgttga agatattcga
    28741 atagcgacag aagagctggg gatttttgca gaaatctatg gacggccaaa acacatttat
    28801 tcaatctatc gtaaaatgaa agatcagaaa aagcagttca acgaaattta tgacttgcta
    28861 gctattcgag tgatcgtaga ttcaatcaag gattgttatg ctgtattagg agcaatccac
    28921 acaaaatgga aaccaatgcc cggcagattt aaggattata ttgccatgcc aaaagctaat
    28981 atgtaccaat ctttgcatac taccgtgatc ggtccggcag gaaatccggt agaaattcaa
    29041 atccgaacac aggaaatgca tgaaatcgct gaattcgggg ttgctgcaca ctgggcctat
    29101 aaagaaggaa aaaatgaaaa agtagaacca gatggtatga cgaaacaatt aagctggttc
    29161 catgagatac tcgaacttca agacgaaagc tatgatgctt ctgaatttat ggaaggcgta
    29221 aaaggagata tctttagtga taaagtctac gtcttcacac caaaaggaga cgttactgag
    29281 ttaccaaaag gatccggacc attggacttt gcatacagta tccatacaga tatcggtaac
    29341 aaaaccactg gtgcaaaagt aaatggcaaa atggtgcagc ttgattacaa attgaaaaac
    29401 ggagatatca ttgagatcat gacttctcca aattcatttg gcccaagtcg cgactggttg
    29461 aaattagttg ctactagcaa agcaagaaat aagatcaaac gtttcttcaa agcccaagat
    29521 cgagaagaaa atgtgatcaa aggccacgaa tccgtggtca aatgtattac agatctagga
    29581 tttacgccta aagatatttt gacgaagaac aaactgcaag aagcactcga tcgttttaat
    29641 tatcaaacag aagatgatct ctatgcagct gtagggtatg gagaagttag ccccttgacg
    29701 atggccaatc gtctgactga aaaagaacgt aaagaacaaa aaatcgagca gcaaaagcaa
    29761 gaagcagaag aaatcatgaa tcagccgaaa aaagaacctg acaaaatgaa agtacgtcat
    29821 gaaggtggcg ttgtcattca aggggtagaa aacttactaa ttcgtatcag tcgctgctgt
    29881 aatccgattc ctggtgatga tatcgttggt tatatcacta aaggcagagg gatatccatt
    29941 catcgtcgag attgtccgaa tgttcagcct gacaaaccaa atgtagcaga acgtttgatt
    30001 gaagtcgaat gggaagatac atcgaataca cgaaaagagt atgatgcaga tttggaaatt
    30061 tacggctata atcgttcagg cttattgaat gatgtacttc aaacagtcaa tgcgctaacg
    30121 aaaaatctca acagcgttga agcacggacg aataaagata aaatggcgac gatccatttg
    30181 acggttggta tccagaattt atcccaccta aagagtatcg tggataaaat caaagcagta
    30241 cctgatgtct acagtgtacg ccggacgaat ggataggagg acagatatga gagcagttgt
    30301 tcaacgggta agtaaagctg ctgtgtcagt cgatcagcaa gaaattggaa aaattggcta
    30361 cggcctactt gttttattag gcattcatga aaccgatacg caaaaagatg tggactatct
    30421 ggtaaaaaaa attgcacaaa tgcgtatttt tgaagatgaa caaggaaaaa tgaatattag
    30481 tgtagaagat gtgggaggac aaattttaag tatctctcaa ttcacactgt ttgcggatac
    30541 gaaaaaagga aatcgaccaa gctttgtgga agccgcacga ccagaaacag ctattccttt
    30601 atacgaatca ttcaatgaag gaatcagaag tcgcggcatc acagtcgcta ccggacaatt
    30661 cggagcagat atgtcggttt ctctcgtcaa tgatggacct gtaacgatca ttattgattc
    30721 taaaaacaaa taaagtaatc tggttttcac tagtaaaaac tatgaaacac gtcgcttaag
    30781 ttagactgat aaactctaac ttaagtggct tttttgtctg caaaaagcgc ttcttttgtc
    30841 agcagtcgga aaaaaagtta agcttaaatt atacaagtta ttttttatag gaggttgctg
    30901 aagatgaaag aagtaccagt agtagatatc aaaatcacag atttaaaaaa attgtatcaa
    30961 aaaggataca acatgttgga ggagttacgc catgaagcgg acgctcccgt cgtcaaagca
    31021 aagattttca ataaagaagc tatcacgatc tacggatcat cagcagcaaa agtattttat
    31081 gatccaagga atttcaaacg aaaaggtgct atgccaaaat tagtattgaa gacacttttt
    31141 ggtcaaggtg gagtacagac tttggatgga gctgctcatc accatcggaa aaatattttt
    31201 atggatctaa tgacaccgga aagaatggaa gattatcatc gtatcttaga taagaatctg
    31261 acacaagcac ttgaagcaca gcatggtcaa tttgaactat ttgatttaag taagatggtt
    31321 ttctttacat ctatttgcga gtgggcaggt atcaatttat cagcgatttc taaagatgag
    31381 gtcgaaaaat tagcagaata tcaaatctct atgatcagcg gaacgtttac ttctccgatt
    31441 gatcacataa aaggtgtcga gaatcgcaaa aaatcagaaa aatgggcgca aggcttgatt
    31501 gaagaagctc gacaaaatcc tgtggctgga aaagaaaatg tggcattata tgcttttgcc
    31561 aatgccacag atttagatgg tcagctttta cctctggaag tggcagctgt tgaattattg
    31621 aacatcatcc gtccaacggt tgctctaact gtgtgggcgg cattgatggg acatgcactt
    31681 ttcagtagac cagatttgta tcagcaatta aaaaatgact tttcgacgtt acaagatccg
    31741 tttattcaag aaatgagacg ctattatcct ttcttcccaa tgctacctgc gatttcgctg
    31801 aaagaagtag aagtagatgg ttatcgtata ccagaaggca gttgggttat tttagattta
    31861 tatggaacag accatgatga acgaacagtt gaagcgccag attcctttat gatcaaacgg
    31921 tatgtaggga aagcaaaaga tatttcctat aaagaagaat atgagatgat tgcacaaggc
    31981 gggggaaatt ttcgacaaat gcatcgctgt gcaggagaat ggatcactct tcatagtcta
    32041 cgtgtgtttt ccgatcagct agtaaataag tttgagttct ctgtacctga acaagattgg
    32101 acgattccat tcaatcagtt tccgacttat ccgaatagcc gagcgctact ttacaagaat
    32161 tagattagca taaacttatc gaaaaaaagg aagatcaaac agtgctgatc ttcctttttt
    32221 catattacta taaattgata aggtcaattg atcaaaatcc acgaaatgct tcatcaatgg
    32281 tttgataatc ttcattggat aaatgcaaat ctaaagcttg gacattgtca gatacttgct
    32341 ctggacgctt agctccaggt ataactacag acactcgtgg attcttcatg taccaagcta
    32401 aaacaagttg cgcaggagtg gcttggtatc ttttagcaat ggggcgaagt acatcgacag
    32461 cggttaaaat ttctctgaaa cgttctcctt ggaaatctgg atttcttttt cgaggatctc
    32521 cttcaccaaa ttgtttttcc tcaccaagtt catattttcc tgtcagcaaa ccggaagcaa
    32581 gaggaaaata gggaacaaaa ctgattttgt ttttttcaag ataagggaat aattcttttt
    32641 cagcctgccg atggatcaaa ctgtatttat cttctactac atcgacatat ccatctgcat
    32701 tcgcttcttt caattgttct aacgtgaagt ttgacacacc gactgctcgg atttttcctg
    32761 cttcttttaa ttcgtgcaat gtagcaactg attcattttt tggtgtgctt tcatccggaa
    32821 agtggatata aaaaatgtcg atgtaatcag tttgcagacg ttttagtgca tcttccaccg
    32881 attgtttgag aaactcagga gaattatcaa atgttcgccc tttatttggt acatgagcag
    32941 cttttgtagc gatgacgaca cgtgagcgat catattcttt ttccttcagt acctcgccaa
    33001 tcagttcttc ggaacggccg tttccgtaag cataagcggt atcgatcaaa ttgatcccgc
    33061 tattcaaagc tgtacgaact acttgttttc ctgtctcatc ttccaaaccg tcaaaaagat
    33121 tatgtcctcc aacggcattt gctcctaatc caagctgttc tgcgtagact tgtgaatgtc
    33181 cgattcttat ttctgccata gctgattcct cctttttgtc tagtctaaaa caaaatacca
    33241 aaaagctgaa ctaaaacgtt tttgtgaggg gaaatagtca gatatctctt gactttgaga
    33301 ctggttttta gtaggttata agtaatatcg aatgtcaatg aaagagcgag taagtttttg
    33361 aaactttaca agagaggatc atcgaggctg aaaatgatct aaagtttcat tagcctaaag
    33421 acactctgga gatggacgag tgaaaagccg tcccgcgtac gagcgttatt cgtaagagga
    33481 aagagaaatc tttcaaaatt aggtggtacc gtgtatgaat ttcattcacc cttgttcatg
    33541 atgaacaagg gtgatttttt tatttacata gctaagaaag ggagagaaaa ctatgagttt
    33601 ccaaagacca aaaggaacaa atgatatttt gccaggcgaa tcagaaaaat ggcagtttgt
    33661 cgaagaaaca gcacgtttat tattcagaga ttatcagtat aatgaaatgc gtacaccgat
    33721 ttttgaacac tatgaagtga tatcccgaag tgtaggggat acaacggata tcgtttcaaa
    33781 agagatgtat gatttttatg acaaagggga acgccatgtg acgcttcgac cagaaggtac
    33841 agcaccaatc gttcgttctt ttgttgagca taaattgttc ggcccagagt atgcgaaacc
    33901 gttcaaaact ttttatatgg gaccaatgtt tcgctacgaa cgccctcaaa aaggacggtt
    33961 acgtcaattc catcaaatcg gagtagaagc atttggatca gaaaaccctg caactgatgt
    34021 ggagagcatg gtgatggcac ttgatttctt caaacagttg gggatcacgc aaatccggct
    34081 agtcatcaat tcattaggtg ataaagaaac gcgtcaagcc tatcgtcaag cattgatcga
    34141 ctatctttta ccttttgaat cagagctgag tgaagactct aaacgccgcc ttcatgaaaa
    34201 tccattacgc gtattggaca gtaaagacaa acgagatcaa aaatttgtgg cagaagctcc
    34261 atctatctta gattatctaa gcgagcctgc tcaaaaacat tttgataccg ttgtttccat
    34321 gctggatgca ttggctgttc cttatgaaat cgacagtaat atggttcgcg gactagatta
    34381 ttacacacat acgatttttg aaatcatgag tgacgcacca aaaatggggg cacaagcaac
    34441 gatttgcgca ggcggacgtt atgacaatct agtggaagaa ctaggtggtc cttcaacacc
    34501 tggcttcgga tttgcaatgg gtatcgagcg attactcttg actatggagg cagaggaagt
    34561 agttatccct gcatttaatg agttggatgc ttacgtggtc gcactcggag acgaaacaaa
    34621 tatcgaagca ttgaaagtgg tccaagcgat tcgaaacttt ggtttttcag ctgaccgcga
    34681 cttcatgaac cgtaaagcaa aagcgcaatt taagacagca gataaagcga atgccaaatt
    34741 agtattggtg atcggtgaag atgagcttgc tagtcaaacg attaatgtca aagcaatggc
    34801 aagccgaaaa gaaaaaagtt ttccattaga agagatatat gaacattttg atgaagtcta
    34861 tgatgaaatg actttattcg agtaggagga aaaaaataaa tggcacaaag aacgatctat
    34921 tgcggattag tttccgaaca acaattagaa caaacagtta cattaaaagg atgggtacaa
    34981 aaaagacgag accttggggg agtaatcttt atcgacctac gtgatcgtga aggaatcgtt
    35041 caaatcgtct ttaatcctga aatcaacaaa gaagcatggg aaatcgcgga taaatgtcgc
    35101 agtgaatacg tgatcgaagt gactggggaa gttagaagac gcggagaaaa tgcagtaaat
    35161 cctaaaatgg ccactggtga attcgaagtg attgcaagtg aaatcgtgat tttgaatcaa
    35221 gcaaaaaccc cgccatttca aattgaagat gatcaagcaa tcagtgacga aatacgtttg
    35281 aaatatcgtt atttggatct gcgtcgtcca aaaatgacta aaaacatcaa attaagaaat
    35341 gcgacaacaa aagcgattcg tcattattta gatgataatg actttctcga tatcgaaaca
    35401 ccttacctag ggaaatctac accagaaggc gcaagagact acctagttcc ttcacgtgtc
    35461 catgctggac atttctatgc gttgccgcaa tctcctcaat tgtttaaaca attgctgatg
    35521 aatgctggat tcgaccgtta ttatcaaatc gttcgttgtt tccgcgatga agatttacgt
    35581 ggagaccgtc agcctgaatt tacgcaagta gatattgaaa caacgttctt aacagcagaa
    35641 gaaatccaaa cttacacaga gggattgatt gcgaaggtaa tgaaagaagt tcgcggtatc
    35701 gaagtcacat tgccatttcc tcgtatgacc tatgatgaag caatggctcg ctacggcagc
    35761 gacaaaccgg atacacgttt tgatatggaa ttgatcgatc taagcgatac agtcaaagaa
    35821 gtagaattca aagtcttcca aatggctctt gaaaacggcg gtgtcgtgaa agcattgaat
    35881 gctaaaggcg ctgcagatcg ctactcacga aaagacatgg atcagttagg tcaatatgta
    35941 ggacaattcg gagctaaggg gcttgcatgg ctgaaagtag aagaagacgg attgaaaggt
    36001 ccaattgcta aattcatggg agaagcaaca gaagcaatca ttaaagcaac agatgcaaaa
    36061 ccaggtgact tgctgatgtt tggcgcagat aaaagtgaaa tcgtcgctgc tgcattaggt
    36121 gctatccgga cacgtttagg aaaagaatta ggactaattg atgaatcgaa attcaatttc
    36181 ttatgggtaa ctgattggcc acaatttgaa ttttcagaag aagaaggtcg atatgtctct
    36241 gctcaccatc cattcacgat gccaaaagaa gaagatatcc cgttattggc tgaagaccct
    36301 gcaaaagtct atgcagaagc ttatgatatc gtcttgaacg gttatgaatt aggcggaggt
    36361 tctatccgta ttcatactcg tgagcttcaa gaaaaagtac tagaaactct aggctttaca
    36421 aaagaacaaa tggaagaaca atttggtttc ttgttaacag ctttagatta cggattccct
    36481 ccacatggcg gtattgcatt aggattggac cgttttgcaa tgttattagc cggagaagat
    36541 aatattcgtg aagtcattgc ctttcctaaa aacggaaaag cagcagatgt gatgagtgat
    36601 gcaccaagta tggtttcgcc tttacaattg ttcgaattga atattgatgt aacgaatata
    36661 gaagaatagc cgataaaaag gtaaaaagga tttgtcgaat acgcttcttt tgacaacctt
    36721 gatttaattg aaaaacactt tataatgaag ccataacata taaagcgggg cttttccatg
    36781 aaaaaaacat tcgaaaattg ggcttatcat gattacacga ccaagctttc ggtatctatc
    36841 gtttatgcgt tgatcgcatc agtcgcattg aatttctttt atcagccagg acatatttat
    36901 tcaagtggta ttactggtct agctcagatt ttgacgactt tgtcaaaatc actgatagga
    36961 ttcgaagtac cggtatcaac aactttgtat ttactgaata tacctttgtt tgtattggcg
    37021 tggttaaaaa tcgggaagaa gtttacaatt ttcactttta tcacagtcac tttgacctcc
    37081 ctttgtatcc atattttacc ggaaacagtc ctatctaccg atccaatcat ctgtgcgttg
    37141 tttggggggg ctgtgatggg actaggtatt gggctagcgt tgagaaacgg cctatcatcg
    37201 ggcggtctag atatcgtgag tatcacgatt cgtaaaaaaa caggaaagtc gattggttct
    37261 atttcgatct acttcaatgc attgatcgtt tttgttgccg gaattctatt tggctggcaa
    37321 tatatgtttt acagtacgtt gtctattttc gtcagtggaa aagtaactga tgtcgtttat
    37381 actaaacaaa agaagatgca ggtaatgatc gtaacgaaac atccagaaaa agtagtaaat
    37441 gaaatccaaa actgtatgcg tagagggatc acgatcatca atgaagcaga aggtgcttat
    37501 aagcatgata agcagacagt attgatgacg atcgttacac gatacgaact gcctctactt
    37561 agagaagcaa tgaaagcttc tgatccacaa gcttttgtaa gtattgcaga aaacgtccag
    37621 atacttggtc gtttctatga ggatggcatt tagatcgagt ttgacaaaaa ttcatgaaat
    37681 actgctgtag acagacgttc gtttgtctgc agtttttttc taccatagta gaatcagcaa
    37741 agaaaaactg cttttttatt tgaaattttc ttaaagttga aaaaaagcat gtttattttt
    37801 ttaaatttat gggatgatag aaaaaaatag tagagaaagg acacgattct acttgctatt
    37861 ttcacttaat acccgtatat tacgaatgaa aaaagtttat gaaaagttgg aatattgaat
    37921 ggaaaaacaa aaaaggttaa aaaacagccg ctacatcact gggtttgatg ggctccgttc
    37981 attagctgtg gtgggtgtta tcttctatca ccttctgcca actagtatga aaggcggcta
    38041 tttgggcgtt ccgatttttt tcgtcgtatc tggttatttg atcacggatc tgctgcggca
    38101 ggaatgggaa caaaatggaa aaatcaatat ttggcaattt tatgtcagaa gaatgaagag
    38161 actctatccg gcacttgttt ttatgcttgt gacagcttcg gcttatatca ctttgtttca
    38221 gcgagggctg ttaaacaatc ttcgaggagt tgtggtcagc agcttactgt atgtcaacaa
    38281 ctggtggcaa atcaataacg gtttatctta tttcgatcga tttgctaatg agtcaccgtt
    38341 tacgcatatc tggtctttag cagtagaagg tcaaaactat ttggtttggc cgatcttgtt
    38401 tgtattgttg atggtatttg ttcgtaaaag aaaatggatc ttttatactg tgttaggtac
    38461 atccttcctc tccgccatct tgatgatgat cttgtataca ccaggaggcg atccaacccg
    38521 tgtttattac ggtaccgaca ctcgtctgtt ttctatatgg ttgggaagtg cgcttgcgtt
    38581 tgtttggcca agtacgcgtt taaagaaaaa catcccgaaa caagcgaaaa atattctgaa
    38641 tatcgctgga ggaatctcgc ttttggcact gattctttgc ttcttctttt tagatgatca
    38701 ttacaatttc atctattacg gaggaatgct cctagtgagt ctgttatgcg tagtgctagt
    38761 tgcagtgaca gcacaccccg gagcaagttt gaatcgatgg ctgacaaatc cagtatttac
    38821 ttggattggc aaacgaagct atggcattta tctgtatcag tttccagtta tgatttttta
    38881 tgaagcaaag gtgactgata ttgccgatca tgttttgttg catactttag tggaaatcac
    38941 attgattcta atcatcagtg aactttctta tcgatatgtg gagcgtccgc tagcacgttt
    39001 tgattatggt cgtacattcc aagtgatgaa aggatggttc agcaaaccga tcatcagtaa
    39061 gagcaaacca tgggttgttc caggcacatt acttgtagca attgcactcg ttggtttcgt
    39121 tacggcaccg aaaaacgctg taacagccga tcaaaaacaa ctgcaggcaa aaatcgctga
    39181 aagtaaaaaa ttagcagaag aaaccagaaa caattcagaa aacaccacta gttcggttga
    39241 ccaagcgatc cttgataagt acgagttgtc caaggcacaa gggaataagg cacaaaaatt
    39301 agaactaacg gcatttggtg attcagttat gttggatgct gcaacggatc tacaagaact
    39361 ttatccaaaa gtagtggtag atggagatgt cggacgacaa ttatatgcca gtgtaccgta
    39421 cattgaaaaa ttaaaggaac aagatttgtt gaaagacact gttctgattg gactaggaac
    39481 gaatggctca ttctcggaag ctcagtttga tagtgtgatg gatgcaatcg gtgatcgaaa
    39541 agtatactgg gtcaatgtcc gagtaccgac gaagcgctgg caaaatgacg tgaatgcgat
    39601 gcttgaacag atgacgaaaa aatacgacaa tatgacgctg attgattggt acagctacag
    39661 taatgaccat agtgactggt tttatgatga tcaagtccat ccaaatcctg aagggatgac
    39721 acattacatt cacttagtca gcgaagcctt gctaggagga agttcaagta cgaacagcag
    39781 tgagacaaac gtgacagaat gatagctaca aaaaacttca ggcaaaaaca tttgtttttg
    39841 cctgaagttt ttttatattt accgtgtttt taacattcat atagattcag gaagcaaaac
    39901 ggttgagtgt gtctgtaaat aacgttcttt ttgttttcgg gtgagttttt tgaatgcagc
    39961 ttctgctttt gttgcttcgc ttcttgaaga aaatacttct gcatggatca tcttaacagg
    40021 tcttcttttt tgaagacgag tatatttagc accagtacca ttgttatgtt cctctaatcg
    40081 tctagaaaga tcggttgtat atccaccata aaatgtctga tctttacaga ataatacgta
    40141 aaaataatgt tcatttgcca tagagcatct cacgaatctc tgcagtgtat tctccatcca
    40201 tattgtaagt gatcaatggt ggagcgactt tgaatccgtc taatttgcct tctttgattc
    40261 cttcaactaa caaaatattt gcctcttttc caggttttgg gtaaacaaat tggatccttt
    40321 taggtgcaat tcgatagtct tccatgacct gaatgatttc taagaaccga tcaggacgat
    40381 ggaccatagt aaagcgtcca tttgttttta gtagcttact tgaaatatcg atcacttctt
    40441 ctaacgtcgt atgaatctca tgacgggcga ttgccaggtg tttattaggg ttcgttttat
    40501 ttgttggcag ccctttgaaa taaggtggat tacagacgac taagtcacaa gaattatgtc
    40561 gaacgacaga taagctgtct ttcagatcga tggtgtggac agtcatttgt tcatttaaat
    40621 ggttaagcgc gatactacgt cttgccatgt ctgctagacg aggttgaagt tcgatagcgt
    40681 cgatagccgc ctgggttcgt ttacttaaaa aaagtccaac tgcgccattt cctgcacaaa
    40741 ggtcaacaat cttccctttt ttaggtacac gggaaaagtg agcaagcagc acagcatcta
    40801 aagaaaaaga aaacactttg gaactttgga taatcttgat atcttctgcg tataattggt
    40861 cgattcgttc gtcttcgtga agcatggatt ccctccaaaa tgaaaaaaat agtcgtctgt
    40921 attatactat ttttattaaa aagaaccaag gtggcttgtc aaacctctat tttttatgca
    40981 tactatgacc ataagaaggg aaaggaagag actatgtttt atcgatttat gcgcggggta
    41041 gttcgagtac tattagcctt agttaatgga aatgcccgct atgagaataa agaagtttta
    41101 ccgcaagatg agaattacat actagttgct ccccatcgta cttggtggga cccattatat
    41161 ctggcggtag ctgcagcacc caaggaattc agctttatgg caaaagaaga actgttcaaa
    41221 aatccagttc tgcgatttat attaactcga tccaatgctt ttccagttaa aagagaaaat
    41281 ccaggaccaa gcgtcattaa aacacctgta aagattttga aatcgacaaa tttgagtttg
    41341 atcatgtttc ccagtgggac aagacatgct acagaactta aaggcggaat ggcattgatt
    41401 tccagaatgt ctaaagcgaa aatcgtgcca gctgtatatc aaggtccgtt gacacttggg
    41461 gatctgttca agcgtcagag agtgacagtg cgtttcggcg aaccaatcga cttatcagat
    41521 atcaagaaaa cggataaaga aggtatggcg gagatcgaac gacgaacaca agcagctttt
    41581 gacaaattgg acaaagaagt gaaccctgat ttcaaatatg aagtcaaaaa aaaataaaat
    41641 gataagttta tgcgtgggcg gtcttttgaa tctactgtcc tgttcagttc agactttttc
    41701 aatgaaaacg agggagagac gtcaagtctg ccctcttttt gcgttccaat atgtaaaata
    41761 gaagacaggt gtcatgtaat ggatcaaagt gttataataa ttagaaggac attttttgag
    41821 gtgattgaat gcgtttttta catacagccg attggcatat cggaaaaaaa ctacaaggtt
    41881 atgacttgat tgaagaccag cgtcatgtac tatcttctat actagatatc gcgaaagaag
    41941 aaaaagtaga ggcaatcgtg attgccggag acttatatga taggagtgtc ccagctgttg
    42001 atgccgtaga attatacaat gaattgatgg tcgattggaa tttaaaggaa aagatcccga
    42061 tttttgctat atcaggaaat cacgatagca gtacgagact ttcagcgggt acgccatggt
    42121 tcagttattc gaactatttt ttgcgaacac agctagcaga agcatttgaa ccagtcgagt
    42181 ttaaaaacgt acaatttttc tttcttcctt actttgaacc aattgcagcg agattatatt
    42241 ttgaagatga caatatccga acgatcaagc aagcggtagc caaggtgata gataaaatgc
    42301 aggagctatt tgattcagaa aaaaaacaag tattgattag ccattttttt gtaatgggaa
    42361 gtgtaaaatc tgattcggaa acgaaaatcg aagtaggcgg gttggattcg atccctggtg
    42421 aactacttac accgtttgat tatgtagcac taggacattt gcataatcaa gctgctttga
    42481 gacaggataa tgctcgctat agcggttccc cattgaaatt ctctttatct gagatcaacc
    42541 aaaaaaaagg cgtatggatc gttgacctga caacaggcct ggagttgaat tttagggaga
    42601 tcgaacccct gcatgatatt gaagaaataa cagcgagttt caaagaattg ctggatcctg
    42661 ccttttatca gtccgtcaat cgggagaatt acatctatat ctatctcact gatagaacga
    42721 tcatcccgaa tatgatgaac cagcttcggc aaatatatcc tcgtattttg gggattgaac
    42781 gggcaaacgg tcgagcagaa caaacaaagc gcaaacgaaa agtactagaa atgaaagatc
    42841 ctcaaaaatt agcagaagca ttttttagtg aaatgacgga agagcagcta actgcaaacc
    42901 aagaaaaatg gctcggagaa acgttgcaag aaatcatgga aatggaatga tcaagaatga
    42961 aaccgaaaaa attacaactt aaaaattttg gtccttttat caatgagacc gtagattttt
    43021 cacgtttgac agaagcaccg ctttttttga tcagcgggaa gacaggagct ggcaaaacga
    43081 cgatttttga tgggatcacc ttcgcacttt ttggtgaaac ttccggaaga cttcgctctg
    43141 gaaaagagat gcgttctttg tttgctacac cagacgaaga aacaagcgtg actttttctt
    43201 ttgaacatca gcagatgagc tatgaaatcg aacgaaaacc agaacaagtt ttagcaaaac
    43261 gaaaaggaaa cggaacaaaa aaacaggcag ccaaagtaac gttgaccatt tttgatggga
    43321 aaggaaaaga aataaaacaa atcacaaaac gtacagaagc agatcagttg atcagagaac
    43381 tgatgaatct cgatgcgaaa cagttttctc aaatcgtttt actaccccaa ggagaattcc
    43441 gtaatttttt ggtttcatca agcagtgata aagagactgt tttacgtcat ttattcggta
    43501 ctcaattttt ccaacagttc aatgagcgat taaaagaaaa agcaaaacaa cagcaacaaa
    43561 aattggatca tttagagcaa gagctgcatc ttctgcaaaa aaggtttgtt ttagtcaaag
    43621 aagaggaacc agcagcaggg tttgaagccg ttttagccca atggacaaac catcaggcaa
    43681 tactcgaaaa acaaatcacg gaagaaaaag aagctctaac ggaacttcag gaaaaacaaa
    43741 agcaactaga aaattcctac tatcgttttg aaagcttgaa aaaaagttat gaagaaaaaa
    43801 cgatattaat aaacaagcaa aaggaattag ctgctcaaaa agacgaaata gaggaaaaga
    43861 aacgatggat tgggtattat gaatttacgc aacgattagt cgaaccaatc cagcgcctac
    43921 aagaaataaa agttgaaaaa atagaactgt cacaaaaaag tagtgagcaa acaaagaaac
    43981 tagcgatgac tacgcagaat tatgaagagt ggcaaaaaaa tgaagcaaac cgcgtacgcc
    44041 gacaaacaaa aatcaaagaa gctgctcaaa aactgcaaaa caaacagatg atggtgccaa
    44101 ttgtcgaatc cttacgtagt aaaaaacaag acgcagaaca attaacaatt tcattggata
    44161 aagaagaatt gaatgccgag aagctgtatc aaaatctcgc tatctatcag gaaagaactc
    44221 gaattcttca agaaaattta ctggatcagg aaaaactgca agaagatcgt cttgcttttg
    44281 gacagttaaa atatctagat gaaaagcagc aagaaataga aaaacaaaaa gcaaaagaca
    44341 ctgggtcgct cgcacagata agagaagaac tcaaaaaagc aactgaagaa ataacaaaaa
    44401 atagtcagct attgaaagaa cagacagaac aagcagaaca gctgaaaagc caatgggcaa
    44461 agcagcaaat tgctcgtctt cagctttttt tgcgtccagg ggagccatgt cttgtttgtg
    44521 gatcaacgga acaccccata caacaattaa aacacgaaca gccaaataag gaagatatct
    44581 taaaagtaga acaacaactg gcagaagctg agtctgctgt agaaagaact cagcaaatca
    44641 ttgccaaaag tgaagcacat gaaaataatc ttcaacaaca attatcagat ttagaacaaa
    44701 aaatcatggc aacgaatata caattggctg aacaagaaga acacttgcaa caggctttga
    44761 aaaaggcatt tcctgagata acagaacaaa aaacaaaaac aactcttgta gtaatcgaac
    44821 aatctttaga tagacgaaaa agcaagatgg ccaatgaaag aatagagctg gaagaaataa
    44881 gacaacaagc agaaaagctc gaacaacaat tagctgtcta caaacagcag cagacagaaa
    44941 aaaataatca gctccagcaa ctgcaaggag aaatcaaaag tttagaacaa caattgcaag
    45001 atacggatag taactgttta gaagaggaga tacgacgact tgaaaaacag ttagaagaag
    45061 actcgaatca actagctgtt gagcagaaag caggagtgca attgttgcaa gatcttcata
    45121 cgcttcggac acagcttgat cttcaacatg aacaaataga aaaaatagat gaaaaaatag
    45181 aaaaggaaca agaaagaata gctaaaaagt tagaaagcca gtcgtatttt tcaacaattg
    45241 aagaaatctg tgcatttgca caacagcaag cacaatataa agaaaaccaa cgtacagtta
    45301 cttcttatca tgaagagcaa ttagtgacag ctgaccggct gcgtcaatta gaaaatatcc
    45361 agctaactga gacattccca gatacagaag agatcagcca acagttagaa gaaataagac
    45421 gtatgatcga aataaagcaa gaaaagatat acggtttaca agaacaaaaa attagtaatc
    45481 aaaaaatcta cgatgaatgt ctttcgattt atcacacaag ccagacgaaa ttagatgagc
    45541 ttacgcaatt acagcagctt tcgcaaacat tcaacggaga aaatcctaag aagactagtt
    45601 tggaacggta tgtgcttcag gtttatcttc aagaagtgtt acaagtagca aatgatcatc
    45661 tccaacgttt aacgaaaaat cgttatcaat ttgagctagc ggataccatt ggcagttatc
    45721 gagggaaaac cggattagaa atcaatatct atgatgatca ggcaggtatg tctcgaagtg
    45781 cgcataccct gtctggcggt gagagtttta ttgcagcact ttctttagca ttagctcttg
    45841 ccgatgtgat ccagacacaa gctggtggtg tagcaatcga tgcattgttt attgatgaag
    45901 gatttgggtc attagatgaa gaagctttgg aaatggcaat ggaagcttta gaaacgatcg
    45961 aaagtgaagg aagaatgatc ggcatcatta gtcatgtaag agaattgaaa gaacgagtat
    46021 tacaacaaat aagaattgat acagaaggta gcggacaaag caaaatacgc tatcaacttg
    46081 gataaaaagg gagattggta actatgaaat ggagtattcc agaaaaagtc attgcacgtg
    46141 gacgtcaata cttgcaagaa ggccgagtgt tatcggttgt ccctgaccaa gaagaaaacg
    46201 tgtggcatgc agaagtcttg ggcagtgaat tgtatatggt tgatttggat ggtacagcaa
    46261 aagagataga ttactgccaa tgtccctatt gggaggaaca taaatattgt aagcatacag
    46321 ttgctgtgga actttattta cggtcgaaaa atctatctcg tatcatgaaa aaagaccagc
    46381 caatgctaga accggtaaaa gcaacaagtg agggagaaat cattgcaaaa ggtttttctc
    46441 gtctaaaaac acaaaacaat cctcaagcgg tgcaaccttt gattattgaa taccaagtag
    46501 aaacgattga gactaatcag tatcacccag agctttctat tttggcactt tacttgcgta
    46561 tcggttcatt ggcatcaccg aaaaaaacat atatcgtaaa aaacatctat gattttttac
    46621 aagcttacac agaaaaagaa acttacactg ttaataaaca gtaccagttt cgtctagata
    46681 aacgggcgtt tcaaaaagaa gatcaaaaaa tactcacgca tcttgcagga agtgctcaga
    46741 cgcatcaatt attaggtacg aacggcttac aagtcaaagg aaaactagat aaaagatatc
    46801 tgcttctccc agtagaacaa gcccgcttcc tgttagagca gatgaatcag actacacgtt
    46861 tgcatctgca gataaatgaa caaaacgatc aaggaataca attttctgat caaaacaaac
    46921 ctttatcatt caaagtggaa aaatttggag aagattattt gctgcaagca ttgaatgatt
    46981 ttgatttttt ctttatgcat tatcagtggg gagttatcca agggacattt tattctctga
    47041 cgaaatatca acaaacggtt tatcaaactt ggaaacaatt gattcgccga ttggagaaac
    47101 cagaagtagt gttcaagaaa aaagaattac cgtctttgtt taaagagatt atccctctgc
    47161 tgtcagagat cggcgacgta gctattgccc ctgcagtaga agaggaagta gcagatatcc
    47221 ccgtcgaatt tattttcttt ttccgaaaag caaaaggaaa aatccaagct cgagtcgatt
    47281 tcgtctatgg tgaagtcatc tattcgactg acccgaagca tgaggtctcg acagaacagg
    47341 cttttcaggt gttacgtgac acagtagaag agaaacgagt acttgatcta ttcagaatgt
    47401 atcgctacca gaaaaatgaa actggttatg aacgtccatt gccaagcggg gaagaactat
    47461 atgccttttt ccgcacagaa ctatctgtct tccgtcagtt tggtgaagtc agacttggaa
    47521 gaaaattgcg agagttgtat ttagatgcgc ataaacatca accgaaaatc gaagtaaccg
    47581 agaccggttc atggctcgat attcgattcg atgtgacagg gatagaggag caagaaatcg
    47641 atcatgtcct gcaaagcttg ctgagaaatg atgcttttta tacattagaa aatggtcagg
    47701 ttctttcttt tgattcagaa gaatttcaac aaacaagcca agtgttgcaa caattacggg
    47761 aatcgatacg tacagaagaa ggaacgatcc atgtacctaa aaatcaagga ttgatcattc
    47821 aaaatcaatt ggaaaaaagc aatgctactt ttagtgaatc gttccaaacg atggtacaag
    47881 atctcattca tccagaaaga taccaggcac agcttcccaa aggattgaat gcaacgatga
    47941 gagattatca aaagcaaggt tttcgatggt tgaagatgtt aggtcattat cagtttggcg
    48001 gtattttagc cgatgaaatg ggacttggga aaacactaca gacaattgcc tttttacttt
    48061 cggaaaaaga agaacggaaa agcttctctg ctttgatcgt agcgcctgcg agcttaattt
    48121 acaactggca ggcagaggtg agaaaattcg ccccttcttt aagtatccaa gtaatcaatg
    48181 ggaataaaaa agaacgtgaa gaattactgg ctaaagacac agatatccga gtgacttctt
    48241 atgccagctt aagacaggat ctagctaatt atcagtcgca aaaaattgat tatttgattc
    48301 ttgatgaggc acaaatggtc aaaaatagca gtacgaaaac tgcacaagca ttgagagaat
    48361 tagctgtacc tcagcggttt gcactgagtg ggacaccaat cgagaataat ttagaagaat
    48421 tgtggtcatt gtttgcaacg attatgccag gatttttccc gactaaaaca aaattcaggg
    48481 aacttgccgc agaagaaatt gcgcaaatga ttcgaccgtt tattttacgg cgagataaac
    48541 aaactgtgtt gaaagacttg ccggaaaaaa cagagatgaa tctatattct gcacttacag
    48601 aagaacaaaa aactgtttat ttagcttact tgcgtcaaat gcgtgaagaa atcacatcaa
    48661 tggattctga agcattcaag aaaaaccgga tcgggatact tgctggtctg acaagactgc
    48721 gacaaatttg ctgcgatcct cgtctgttta ttgaagatta ccaaggcggt tccggcaagc
    48781 tcgaacaagt aaaagatcta ctggtagctg ccaaagagaa caaacgaaga gttttgctgt
    48841 tttctcaatt tacagggatg ctgactatct tgcaagaaga gttagccgag ctagggatct
    48901 cgacatttta tttaagaggc agtacgaaac cacaagatcg gttaagtatg gtagacgcat
    48961 ttaatgctgg cgaaaaagat gtttttctga tttccttaaa ggctggggga actggattga
    49021 atctcacggg agcagatacg gtcatcttgt atgacctttg gtggaatcct gccattgaag
    49081 aacaagctgc tggacgtgca catcggatcg gacaaaaaaa tgtggtagaa gtgtggcgta
    49141 tgatctctga aggaacgatt gaggaacgca tggattcact ccagcaagaa aaacgcgaac
    49201 tgttccaaaa agtgatccaa ggtaatgaag aacagttgac gaaactgact gaagaagaca
    49261 tccgcatgat tttaagtgta ggagaaatcg aggagtaaga gactcgaaaa agcgttcagt
    49321 aatgggtaag aaagcctttc taaagaagcg attgtttatt tgatttaaaa aatagcaagg
    49381 aggctgggat tccattttat cccagcttcc ttgctatcat ttttttattt ctatttttaa
    49441 aggttataca aagattgtct tcattgtctc taacagctga tcaaaagaag aaatctcgat
    49501 atcgcaaatt ggagtctgtt caattttgcg ttctcgatgg ttgaaccaaa gcgcttgcca
    49561 gttagcactt tttgcaccta gaacatcatt gtcgtaatta tcgccaacgt ataaggtctc
    49621 ttctggaagc atatgaaatt ctttctcagc cagctgaaaa atctcttttt ctggtttttg
    49681 gaaacctgtt gcttgagaaa tgagcatatt gcgggaagga atccaattgt tcaattgaag
    49741 ttgcttcagt ttttttgtct gatggtcagt cggcccattt gtgataattc ctaatgggac
    49801 attcttttcc ttcaagaaat cgagggtttt tttgacctcc tcatgcaagc agatattatc
    49861 aagttcttcc tcgtaaattt tttgaaataa gaggccatct tcttccgtaa tatgagggta
    49921 gtctaaatct tttagtgatt ggctgatccg atgtgctcgc atatattcta gtgtccaatc
    49981 tcctgccatt acttttggga agttttcatc actatgatgg cggaaccgga tatatagtgg
    50041 atgcatatct gcatctgaaa caagcggaac gactcttttg accgcgtttc gaaatggctg
    50101 ctgctgatca tacatcgtat catcaacatc aaacacaact gatttaatca aaattttaca
    50161 cgcctttcgg ttttcattga ttttttcaca aatccttgac tattgtaaca gaaaaaattg
    50221 tctaaaaaag ctgataaata aaggtttttc ttactatatt tctatttcct tgacagaata
    50281 aggtttttat atttcagttt ttttcactaa aaatcgtgaa acacgtcaat aagtttgatg
    50341 aacaaattaa tcattatggt agaagtcgat tgatttattt taatgcaatc tctgtggaat
    50401 tttcgaaag
//
LOCUS       DARBET010000012        49215 bp    DNA     linear   BCT 07-NOV-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4027
            SAMEA114569841-rid20112183.denovo.012, whole genome shotgun
            sequence.
ACCESSION   DARBET010000012 DARBET010000000
VERSION     DARBET010000012.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA114569841
            Sequence Read Archive: ERR12203666
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 49215)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 49215)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001972618.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001972618.1
            Genome Coverage       :: 132x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/07/2023 13:18:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,041
            CDSs (total)                      :: 2,975
            Genes (coding)                    :: 2,908
            CDSs (with protein)               :: 2,908
            Genes (RNA)                       :: 66
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 67
            CDSs (without protein)            :: 67
            Pseudo Genes (ambiguous residues) :: 0 of 67
            Pseudo Genes (frameshifted)       :: 26 of 67
            Pseudo Genes (incomplete)         :: 28 of 67
            Pseudo Genes (internal stop)      :: 20 of 67
            Pseudo Genes (multiple problems)  :: 6 of 67
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..49215
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA114569841-rid20112183.denovo.012"
                     /isolate="VRE4027"
                     /db_xref="taxon:1352"
                     /country="Denmark"
     gene            154..966
                     /locus_tag="SCJ86_000803"
     CDS             154..966
                     /locus_tag="SCJ86_000803"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294134.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="isoprenyl transferase"
                     /protein_id="HEG1168385.1"
                     /translation="MLRFFPQKNKYIQEKSEFQFNTDGPIPQHIAIIMDGNGRWAQNR
                     RLPRVAGHKEGMETVKKVTKKASRLGVKVLTLYAFSTENWKRPKDEVSFLMQLPVDFF
                     DTFVPELIKENVKVHVMGYENVLPEHTQDAVRRAIEQTKNNTGMVLNFALNYGSRAEI
                     VTAVQEIAEEVAKGEIHAEEIDDELIAKHLMTGFLPKELQDPELMIRTSGEERISNFL
                     LWQIAYSELYFTKALWPDFDGAHLEEAIASYQNRDRRFGGVKKTEDEKGDQS"
     gene            963..1763
                     /locus_tag="SCJ86_000804"
     CDS             963..1763
                     /locus_tag="SCJ86_000804"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidate cytidylyltransferase"
                     /protein_id="HEG1168386.1"
                     /translation="MKQRVVTAAVALVLFIPIIWIGGIAVELTAAILAVIGVYELFRM
                     KGLTLLSFEGVLSAVGAVLLVLPKERWFFFLPENVSTFMLFYLIVMILLGTSVISKNT
                     YTIDEAGFPVLVSLYVGIGFQNFVNARTESLAVLIFGLFIVWATDIGAYMVGRRYGQH
                     KLSPDISPNKTIEGALGGIGSAVVVAFLYLLLYPAKELFNHGMFVMLLMTIIFSAVGQ
                     FGDLVESAIKRHYDVKDSGNILPGHGGILDRFDSLLFVFPIMHLFGII"
     gene            1924..3192
                     /gene="rseP"
                     /locus_tag="SCJ86_000805"
     CDS             1924..3192
                     /gene="rseP"
                     /locus_tag="SCJ86_000805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306441.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RIP metalloprotease RseP"
                     /protein_id="HEG1168387.1"
                     /translation="MKTILTFIIVFGILVIVHEFGHFFFAKRSGILVREFAIGMGPKI
                     YGHQAKDGTTYTLRLLPIGGYVRMAGNGDDETEMAPGMPLSLLLNSDGIVEKINLSKK
                     IQLTNAIPMELSRYDLEDELTITGYVNGDETEVVTYPVDHDATIIENDGTEIRIAPKD
                     VQFQSAKLWQRMLTNFAGPMNNFILAILLFIILAFMQGGVQVTNTNRVGEIMPNGAAA
                     EAGLKENDEVVSVDGKEIHSWNDLTTVITKNPDKTLDFKIEREGQVQSVDVTPKSVES
                     NGEKVGQLGIKAPMNTGFMDKIIGGTRQAFSGSLEIFKALGSLFTGFSLDKLGGPVMM
                     YQLSSEAANQGVTTVISLMALLSMNLGIVNLLPIPALDGGKLVLNIFEGIRGKPLSQE
                     KEGILTLAGFGFLMLLMVLVTWNDIQRFFF"
     gene            3260..4969
                     /locus_tag="SCJ86_000806"
     CDS             3260..4969
                     /locus_tag="SCJ86_000806"
                     /EC_number="6.1.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723221.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="proline--tRNA ligase"
                     /protein_id="HEG1168388.1"
                     /translation="MRQSKMLIPTLREVPNDAEVLSHQILLRAGYIRQVSAGIYSYLP
                     LANRVLEKLKRIMREEFEKIGAVEMLMPAILPAELWEESGRYETYGPNLYRFKDRNDR
                     KMILGPTHEETFTDLMRNEINSYKKLPLNLYQIQPKYRDEKRPRFGLLRGREFIMKDA
                     YSFHATEESLDETYKDYEKAYTEVFKRCGLEFRSIIGDGGAMGGKDSKEFMAISEIGE
                     DTICYSTESDYAANLEMATSYYVSKKSHETQLELEKVATPNVKSIDEVADFFSVEPQK
                     IIKSVLFIADEQPVLVLVRGDHEVNDVKLKNFLSADFLEEATEEEAQKYLGAEFGSVG
                     PVGVSDDVKVYADRYVQDLANAITGANETGFHYKNVNPERDFTVLSYEDLRFVQEGDP
                     SPDNNGVLAFTRGIEIGHIFKIGTRYSESMGATVLDENGREKFVIMGCYGIGVSRLLS
                     AIVEQHSDEQGINWPKGIAPFDLHIVQMNLKDEYQTNLTNELEEAMTKAGYEVLVDDR
                     NERAGVKFADSDLIGCPIRITVGKKAVDGIVEVKIKKTGEMVEVRKEELVNTLPILLN
                     QGE"
     gene            5181..9539
                     /locus_tag="SCJ86_000807"
     CDS             5181..9539
                     /locus_tag="SCJ86_000807"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002308901.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PolC-type DNA polymerase III"
                     /protein_id="HEG1168389.1"
                     /translation="MSEKRDLFEKLLEQIQLEEAEKNHPLLTAGEMDSVVVHRKSRLW
                     EFTLHFSKILPIMLYRSLTQHLTIAFKDIAQVKLNILADDQTFDEQLLQDYWAQALEN
                     QQCDTPLAQQVLKTQVPVIKDRKVILPIDSTGAISYLKQQYLPLVEELFVSYGFPKFR
                     IEPEVDEQQAERVLKLFEERKQEQAEAFMKQAAESLVVHEQKKKERKEQSPALEGPIH
                     IQLGRNIPADEPTTPMISIVEEERRVTLEGYVFDKEVRELRSKRKILTLKITDYTSSF
                     IVKKFSNGEKDEQVFDAISVGSWLKVRGSIQEDTFVRDLVMNAQDIIEVKHTPRKDYA
                     PEGEKRVELHVHSNMSTMDATNSISDLVAQAGKWGHRAIAITDHGGAQAFPEAHSAGK
                     KAGVKILYGVEANVVDDGVPIAYNDAHEALSEATYVVFDVETTGLSAVYDTIIELAAV
                     KMYKGNVIESFDEFIDPGHPLSRTTVDLTGITDGMVRGSKSEEEVLRMFLEFSKDTIL
                     VAHNAAFDMGFLNTSYARYGIPEAANPVIDTLELARYLYPQFKRFGLGVLSKKFGVSL
                     EQHHRAIYDAEATGHLAWIFVKEAMDNHNMLYHDQLNEHIGEGDSYKRARPFHVTILA
                     KNQAGLKDLFKLISMSNVEYFERVPRIPRSQLKKMRENLLIGSACDKGEIFEAMMQKG
                     VEEARNRAKFYDYIEVMPKAVYAPLIEQELVKNEHDLEEIIQNLVEIGKSLDKIVVAT
                     GNVHYLNEEDAIYRKILINSMGGANPLNRHSLPDVHFRTTDEMLTAFHFLGEETAKEI
                     VVENTNKIADICEEVIPVKDELYTPKIPGSEDEISELSYTKAKQMYGDPLPEIIQKRL
                     KKELNSINGNGFSVIYLIAQKLVHKSNEDGYLVGSRGSVGSSFVATMTGITEVNPLAP
                     HYYCPECQYSEFFEDGTYGSGFDMPEKQCPKCGARLNKDGHDIPFETFLGFHGDKVPD
                     IDLNFSGDYQAEAHNYTKVLFGEDYVYRAGTIGTVADKTAYGYVKGYERDNNLQFRSA
                     EVDRLAKGATGVKRTTGQHPGGIIVIPDYMDVYDFTPIQYPADDQNSEWKTTHFDFHS
                     IHDNVLKLDILGHDDPTVIRMLQDLSGIDPQTIPTDDPEVMRIFAGPEVLGVSQEQIY
                     SKTGTLGIPEFGTRFVRGMLEETHPTTFAELLQISGLSHGTDVWLGNAEELIRRGDAT
                     LAEVIGCRDDIMVYLIHAGLDSGMAFKIMETVRKGQWNKIPDELRETYLSAMKENNVP
                     DWYIDSCSKIKYMFPKAHAAAYVLMALRVAYFKVYFPILYYCAYFSVRADDFDLVSMC
                     KGKDAVKQAMKEITDKGLDASVKEKNQLTVLELANEMLERGFKFGMIDLYKSDAVNFV
                     IEGDTLIAPFRAVPSLGTNVAKQIVEARKDGPFLSKEDLATRGKVSKTLIEYMNDNGV
                     LKDLPDENQLSLFDML"
     gene            9684..10157
                     /gene="rimP"
                     /locus_tag="SCJ86_000808"
     CDS             9684..10157
                     /gene="rimP"
                     /locus_tag="SCJ86_000808"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016625399.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome maturation factor RimP"
                     /protein_id="HEG1168390.1"
                     /translation="MSSVVETVTEMVTPILDEQNFELVEVEFVKEGKSWFLRVFIDKE
                     GGIDIEECAFVSEKLSEKLDAADPDPIPQAYFLEVSSPGAERPLKKESDYEKALGDYV
                     HVSLYQPVDGEKQYEGFLQSFDAEQLTLKIRIKTREKELTFDRKNIAKARLAIQF"
     gene            10180..11355
                     /gene="nusA"
                     /locus_tag="SCJ86_000809"
     CDS             10180..11355
                     /gene="nusA"
                     /locus_tag="SCJ86_000809"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313645.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination/antitermination
                     protein NusA"
                     /protein_id="HEG1168391.1"
                     /translation="MSKEMLNALDALEAEKGISKEIVIDALEAALVSAYKRHYGQAQN
                     VEVEFDQKKGKIHVYAVKEVTEEVMDSQLEVSLKDALLINPAYEIGDTIRFEVTPKDF
                     GRIAAQTAKQVILQRVREAERTIIYNEFSAYEKDIMQGIVERQDKRYIYVNLGKIEAV
                     LSKQDQMPNEFYQPHDRIKVYVSRVENTSKGPQVFVSRSHPDLLRRLFEQEVPEVYDG
                     IVEIVSIAREAGDRSKVAVRSNDPNIDAVGTCVGPKGQRVQAIVNELKGENMDIVEWN
                     EDPAVFIANALNPAQVMDVIFDETNPKACTVVVPDYQLSLAIGKRGQNARLAAKLTNH
                     KIDIKSESDMTEFYENQEQQKETEELHDEAIVQSDLTDDEYETIAFNNETVEEKPEA"
     gene            11377..11670
                     /locus_tag="SCJ86_000810"
     CDS             11377..11670
                     /locus_tag="SCJ86_000810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875920.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YlxR family protein"
                     /protein_id="HEG1168392.1"
                     /translation="MKQRKIPLRKSVVSGEMKPKKEMVRITRSKEGEVSIDPTGKMPG
                     RGAYVSLEPEEVQQAWDKKILDRVLETTLTDEFYQELLDYVTHQKARKELFGK"
     gene            11667..11978
                     /locus_tag="SCJ86_000811"
     CDS             11667..11978
                     /locus_tag="SCJ86_000811"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344352.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YlxQ-related RNA-binding protein"
                     /protein_id="HEG1168393.1"
                     /translation="MNTVNRQKAMNLIGLAMRAGKLITGEELTIAEIRKQKAKLVFVA
                     TDASENTRKKIKDKSSYYEVPCFELFSEAEITQMIGKPRKVFGIMDAGFAKKTKELIE
                     G"
     gene            11991..14297
                     /gene="infB"
                     /locus_tag="SCJ86_000812"
     CDS             11991..14297
                     /gene="infB"
                     /locus_tag="SCJ86_000812"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297399.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-2"
                     /protein_id="HEG1168394.1"
                     /translation="MGNKRIYELAKELKKSSKDVVEKAQQLGIDVKNHMGAITSNDEK
                     KLQQAFKNPSTKQQAAQQASRKPANQQPNDQRKKETTNNQQKNKNNRSYQDRGQGSGQ
                     VNQGKKPTTNQNNTGNTSSQNRQGTSQGGNQNRTNNNNKPNNNNRGKFNNNNRNRYNK
                     KGKKGKQQQSTKPAVPPRKFRELPDVLEYTEGMNVADIAKKIHREPAEIIKKLFMLGV
                     MVNQNQALDKDTIELLATDYGMEPQEKVQVDIADIDKFFEAEEVDPDKLVSRPPVVTI
                     MGHVDHGKTTLLDTLRHSRVTSGEAGGITQHIGAYQIDIDGKPITFLDTPGHAAFTSM
                     RARGASITDITILVVAADDGVMPQTVEAINHAKAAGVPIIVAVNKIDKPGANPQHVMQ
                     ELSEYELIPEAWGGETIFVEISAKFGQNIEELLEMILLVAEVEDLKADPTQRAIGTVI
                     EARLDKGKGPVSTLLVQEGTLHVGDPIVVGNTYGRVRVMTNDLGRRDKEAGPATPVEI
                     TGLNDVPQAGDRFVVFEDEKTARAAGEERAKRALLENRAATSRVTLDNLFESLKEGEL
                     KEVNVIIKADVQGSAEALAASLKKIDVEGVRVKIVHSAVGAINESDVTLAAASNAIII
                     GFNVRPTPQAKVQADTEEVDIRLHRIIYKAIEEIETAMKGMLDPEFEEKITGQMIVRE
                     TFKVSKVGTIAGAYVTEGYIRRDSGVRVIRDGIVIYEGQLASLKRFKDDVKEVKMGYE
                     CGAMIEKFNDIKVDDVIEGFIMEEVKND"
     gene            14324..14671
                     /gene="rbfA"
                     /locus_tag="SCJ86_000813"
     CDS             14324..14671
                     /gene="rbfA"
                     /locus_tag="SCJ86_000813"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735198.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosome-binding factor RbfA"
                     /protein_id="HEG1168395.1"
                     /translation="MANYRDRRVGQEILKEVNDILRKKVRDPRVENVTITDVKVTGDL
                     QQATIYYSILSDLASDKKTAQDGLNKASGLIRRELGHNLSIYKTPELFFELDESVVYG
                     NHIDELLRDLNKD"
     gene            complement(14828..15001)
                     /locus_tag="SCJ86_000814"
     CDS             complement(14828..15001)
                     /locus_tag="SCJ86_000814"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288514.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HEG1168396.1"
                     /translation="MIFLLTVNGPTNSNFYGAGSTDLLVTWLYLTVTKIDYNLASVIG
                     ILIFIYMQKFIYY"
     gene            15449..17811
                     /locus_tag="SCJ86_000815"
                     /pseudo
     CDS             15449..17811
                     /locus_tag="SCJ86_000815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016625383.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoketolase family protein"
     gene            18179..19102
                     /gene="truB"
                     /locus_tag="SCJ86_000816"
     CDS             18179..19102
                     /gene="truB"
                     /locus_tag="SCJ86_000816"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA pseudouridine(55) synthase TruB"
                     /protein_id="HEG1168397.1"
                     /translation="MDGLLPLWKERGMTSHDCVFKLRKILHTKKIGHGGTLDPDVDGV
                     LPICIGKATKVIEYLTDSGKIYVGEITLGFSTTTEDRSGERVETKPVDMSLSEKKIDE
                     VMASFIGEITQIPPMYSAVKVNGKRLYEYARNNETVERPVRKAQIYRFIRTSELTRDE
                     VTGTISWRFEVECGKGTYVRTLAVDTGKKLGYPAHMSDLTRTASAGMSQKDALTLEEV
                     AQAVKNGTIDDCLLPIEKGVERFKRVDITDEVWQKVKNGMRLDYTVFALPEMPSEEIA
                     LFYKGKVVSIYQPNPKEKNKLKPSKVLRNEE"
     gene            19106..20047
                     /gene="ribF"
                     /locus_tag="SCJ86_000817"
     CDS             19106..20047
                     /gene="ribF"
                     /locus_tag="SCJ86_000817"
                     /EC_number="2.7.1.26"
                     /EC_number="2.7.7.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342162.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="riboflavin biosynthesis protein RibF"
                     /protein_id="HEG1168398.1"
                     /translation="MNIIKIRHPYQSEQIPEDDVVLVLGFFDGVHIGHQKVIKTGMEI
                     AQKEGLKLALMTFNQHPSIVFKKIDPSSVKYLTSLKQKEEKMAALGIDYLYEVDFTSS
                     FAHLAPQEFVDQYIVGLHAKYAVSGFDYTYGPKDIADVAHFPEYAKGRFKIVTVSKEE
                     EQGEKISSTRIRGLLDSGQVKEAAKLLGCTYEVEGVVVHGDARGRLLGFPTANVKVKS
                     TVHLPKEGVYVSEIKIGETWYPAMGSIGHNDTFGEGRELTVELYILDFNQDIYGEHVA
                     VRWNQFLRDQVKFSSVEELIEQLKEDEQATAEYFSQQ"
     gene            20399..21184
                     /locus_tag="SCJ86_000818"
     CDS             20399..21184
                     /locus_tag="SCJ86_000818"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340778.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HEG1168399.1"
                     /translation="MYKELENKVAVVTGGSKGIGTAIAQRFGKEKMNVVVNYNSDKEG
                     AEKAVKAIEEAGGKAVSVQADVSSEEGIQRLLDTALENFGTLDVWVNNAGMENQHPTH
                     ELSLEDWERVLKVNLTGVFIGTKAALNYFTENNKKGNIINMSSVHEQIPWPTFAHYAA
                     SKGGVKLFTETVAMEYAPKGIRINSIGPGAIRTPINAAKFDDPEQKELLESMIPLGRV
                     GEPKEIAATAAWLASDESSYVTGITLFADGGMTLYPSFQGGKG"
     gene            21197..22048
                     /locus_tag="SCJ86_000819"
     CDS             21197..22048
                     /locus_tag="SCJ86_000819"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288523.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar transporter"
                     /protein_id="HEG1168400.1"
                     /translation="MGLLIALIPAVGWGIQPLILKKIGGRPTNEILGTGIGAVLVGLL
                     VQLFASPAGISVTTFLISLLSGAFWVIGQTGQYTSFNLIGVSKTMPISTALQLVGTSL
                     IGVFAFGEWSGSMGKIIGAAAIILLVFGSALTAISDDPGKKGGITKGISLLASTSIGY
                     WVYSALPKLVDASGLSIFFPQMIGVFLGAVIYVIFRKPTAFSEDKSWKATIVGAIFSV
                     SALAYIFSANENGVATAYIITQLNVVISTLGGMYILHENKTPKEKKLTIIGLLLIVVG
                     SMVTVFI"
     gene            complement(22172..22624)
                     /locus_tag="SCJ86_000820"
     CDS             complement(22172..22624)
                     /locus_tag="SCJ86_000820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338814.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HEG1168401.1"
                     /translation="MKDFYSVNELAEQLGVTTRSIRNYLHEGKLKGTKVGGQWKFSER
                     NLFEFLYGDQAEEAAKEMQRFMLNAPITMRFNLQYRDFTAINQFREQLVQYHNDVYAN
                     KKDRLLQYDLYKDNHAEILIGGNFNYVVNFSQWINEKLLIQTDISLVS"
     gene            complement(23089..23457)
                     /locus_tag="SCJ86_000821"
     CDS             complement(23089..23457)
                     /locus_tag="SCJ86_000821"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337232.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1801 domain-containing protein"
                     /protein_id="HEG1168402.1"
                     /translation="MEKFQPIINQIKEKEHQQKIKDLFAKITETYPQLDTTVKWNQPM
                     FTDHGTFIIAFSFAKNHFSIAPEKAAIRALEKNIQEAGYVYTDNVIKVPWKSVINWEL
                     IEQLISFNIEDKKGHDKFWR"
     gene            23607..24089
                     /locus_tag="SCJ86_000822"
     CDS             23607..24089
                     /locus_tag="SCJ86_000822"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628262.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prolyl-tRNA editing protein"
                     /protein_id="HEG1168403.1"
                     /translation="MIDLAKKFLHENIYFELYHHRAIYTNEDALVIKKEQGFTGTETK
                     SLFLKDKKDNNYVYLTFTTKQTDFKALGKIVGKRLSIVSSERMEEQTGQKQGAVSPFG
                     YEKQIPIIIDEELLSNEKLVFAPGRPDQTMVIQTVDLNKIIQLLEMPIFLMPANEKNN
                     "
     gene            24126..25136
                     /locus_tag="SCJ86_000823"
     CDS             24126..25136
                     /locus_tag="SCJ86_000823"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LD-carboxypeptidase"
                     /protein_id="HEG1168404.1"
                     /translation="MISYPLLERNATIGVTAPSSGVSEEHHSLLESALKRMEEKSFQV
                     VTGKTLWTQDKEKSGPARKRADEFNQMMQDENIQMIFPTWGGELLIEILDFVDFDALQ
                     PKWIVGYSDISTLLLAVTLKTGIATAHGTNLIDLRGEYLDETTAMWESVLKTREGESI
                     LQQSSEKYQKVWPHEHSSSSVFHLTEPTNWQSVSGDAVRMKGRLLGGCIDTIRHLIGT
                     PYGDVQSFKRKYNDGKPILWFLENCELSSVDLRRSLVQMKLAGWFDGSSGIMFGRSAA
                     EDLLDYKMKDVYEDLSKELKLPILYDIDCGHVPPQLTLINGAYAEVAVEDRKATVLQT
                     FI"
     gene            complement(25251..25964)
                     /locus_tag="SCJ86_000824"
     CDS             complement(25251..25964)
                     /locus_tag="SCJ86_000824"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEG1168405.1"
                     /translation="MKKLGFILLLVSLFLVGCTSPSKENSDPSATTDKSSIRQTSSAE
                     KSSTQKSASTETNTSSTSSKVSEPNTSSTSSSTVETNISSSTIDTTSSQSSQSIDPLA
                     GYSDLQIEYARVWLAIRGNTFRDTSGDFELHVIHYPAGTPIAPYDAGSAVYPKDVVML
                     TGKYSYQGVLVYSSNHDGTITSYPVPSHWQMPADQTSYPAFIRETTQEIIDNASVVAI
                     PTGNPNDVKQLIDVTVIDN"
     gene            complement(26154..26744)
                     /locus_tag="SCJ86_000825"
     CDS             complement(26154..26744)
                     /locus_tag="SCJ86_000825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400305.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine amidotransferase"
                     /protein_id="HEG1168406.1"
                     /translation="MKTAITFLLEEYADWEGAYITSTLNQNEKWQNKTASIQSEVTSI
                     GGLKTKIDYLIEQIPEKVDLFIMIGGNSWDIKNHHLYQIIDKLLKTDVPVAAICGAVD
                     YLAQSGLLQGYKHTGNAQFPWLESENYQNKEDFIEQQAVKDRNLITANGTAPIEFTDL
                     ILQAVHFETEEIREKTIRLYKLGFYDYYKKYGDPFS"
     gene            27228..27455
                     /locus_tag="SCJ86_000826"
     CDS             27228..27455
                     /locus_tag="SCJ86_000826"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenosine deaminase"
                     /protein_id="HEG1168407.1"
                     /translation="MKIAFHIFADFDLMPKVSFAITVYREAVKAPCSSDDNKQAFLEL
                     GFLDSKYAAYFNELVLERDFKKVENRHDFST"
     gene            27471..28637
                     /locus_tag="SCJ86_000827"
     CDS             27471..28637
                     /locus_tag="SCJ86_000827"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299641.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oxygen-independent coproporphyrinogen III
                     oxidase"
                     /protein_id="HEG1168408.1"
                     /translation="MIETINPAENKVSAYIHIPFCEHICYYCDFNKVFLEGQPVDEYV
                     EMLLNEMKIMLESHPTANLETLYVGGGTPTSLSAKQLDRLLSGAREILPFQNGNEFTV
                     EANPGDLTREKLQVMKNYGVNRLSMGVQTFDNRLLKKIGRKHTAEDVYQTMQFLEAEN
                     FTNVSIDLIYALPGQTLEGYLDTLEKALALDLPHYSLYSLILENKTMFMNWVRQGRLE
                     LPDQETETRMFEETIEAMEKHGRHQYEISNFGLSGHESQHNLMYWNNDHYFGFGAGAS
                     GYLGDIRYRNKGPIQHYLRPLRANERPVLEEEKLTRKNQIEEEMFLGLRKKTGITKKH
                     FYDRYQKTIESVYGDVLRELEQEGLLVNEEDRVYLTPRGTFLGNNVFERFLFEK"
     gene            28871..29914
                     /gene="hrcA"
                     /locus_tag="SCJ86_000828"
     CDS             28871..29914
                     /gene="hrcA"
                     /locus_tag="SCJ86_000828"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340771.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heat-inducible transcriptional repressor HrcA"
                     /protein_id="HEG1168409.1"
                     /translation="MLTKRQNDILRLIIQHYTSSGVPVGSKTLMSEGVKASSATIRND
                     MKALEDEGLLLKTHSSSGRIPSALGYRYYVDHLLKPARVAEDDLLLIRQSLGREFHEI
                     NEIIKQSAEILSELTSYTTFSLGPEIKDRRLTGFRIVPLNNRQIIAIVVTDMGNVESQ
                     VFTLPEYMGSQELEKMVQIVNDRLVGDPLMTVYHKLRTEIPMILHKYFQTTEGMSQLF
                     DTVLGHVFEDKVYVSGQMNLLDYEPRQDLDQFKSMFSFMKDSDGLTQMITPMDSNIHI
                     KIGAELGNDLLQNMSMIQASYEIDGHGRGTIALLGPTSMPYSRMFGLVDVYRRELAAK
                     LADYYRSLDLSNS"
     gene            30008..30571
                     /gene="grpE"
                     /locus_tag="SCJ86_000829"
     CDS             30008..30571
                     /gene="grpE"
                     /locus_tag="SCJ86_000829"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotide exchange factor GrpE"
                     /protein_id="HEG1168410.1"
                     /translation="MKENQEELDKEMTDAQPEPEIDVEAAEDSGISEAEAEEFETAKL
                     KAELEEMEDKYLRARAEIANMANRGKNEREQLQKYRSQDLAKKLLPSIDNLERALATE
                     VSDDQGAGLKKGVEMVLESLRNALEEEGIEKIPAKGEAFDPNLHQAVQTVPATEDTPA
                     DTIVEVLQEGYKLHDRVLRPTMVIVAQ"
     gene            30622..32451
                     /gene="dnaK"
                     /locus_tag="SCJ86_000830"
     CDS             30622..32451
                     /gene="dnaK"
                     /locus_tag="SCJ86_000830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016635026.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone DnaK"
                     /protein_id="HEG1168411.1"
                     /translation="MSKIIGIDLGTTNSAVAVLEGNEAKIIANPEGNRTTPSVVSFKN
                     GEIQVGEVAKRQAVTNPNTISSIKRHIGEAGYKVDVEGKSYTPQEISAMILQYIKGFA
                     EDYLGEKVEKAVITVPAYFNDAQRQATKDAGKIAGLEVERIVNEPTAAALAYGLDKTD
                     RDEKILVFDLGGGTFDVSILELGDGVFDVLSTAGDNHLGGDDFDNKIIDYMVAEFKKE
                     NSIDLSQDKMALQRLKDAAEKAKKDLSGVSSTQISLPFITAGESGPLHLEMTLTRAKF
                     DELTADLVERTKVPVRQALKDAGLSQSEIDEVILVGGSTRIPAVVEAVRKETGKEPNK
                     SVNPDEVVAMGAAIQGGVITGDVKDVVLLDVTPLSLGIETMGGVFTKLIDRNTTIPTS
                     KSQVFSTAADNQPAVDIHVLQGERPMAADNKTLGRFQLTDIPAAPRGIPQIEVTFDID
                     KNGIVNVSAKDLGTQKEQKITIKSSSGLTDEEIERMVKDAEANAEADKARKEEVDLRN
                     DVDALLFSVDKTLKELEGKVDEEEVKKAETARDELKAAVEANNIEEMKTKRDALNEIV
                     QNLTVKLYEQAAQQQAQENPEAAQGGADDVVDADFEEVDGDDK"
     gene            32602..33768
                     /gene="dnaJ"
                     /locus_tag="SCJ86_000831"
     CDS             32602..33768
                     /gene="dnaJ"
                     /locus_tag="SCJ86_000831"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321911.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone DnaJ"
                     /protein_id="HEG1168412.1"
                     /translation="MATKRDYYEVLGLSKGASEDEIKKAYRKLSKKYHPDINKEPDAE
                     EKFKEVSEAYEILSDPQKRAAYDQYGHAGTDPNYGAGGAGGGFGGFGGFSGGGFGGFE
                     DIFDSFFGGGGRSVDPNAPRQGADLQYTVDLSFEEAIFGVEKEIKYNREEICHTCGGN
                     GAKPGTQPTTCHKCHGAGTINVERQTPLGRVMSRQTCDVCHGTGKEIKEPCPTCHGSG
                     HEKKAHSVKVNVPAGVEEGQQMRLAGQGEAGENGGPFGDLYVVFRVEESDIFDREGSE
                     IYYELPLSFVQAALGDEVQVPTVHGDVKLKIPAGTQTGTNFRLRGKGAPKLRGGATGD
                     QHVKVKLITPKNLNEQQREALRAFAEAGGIKVEEQQEDGFFDKFKDAFGGKKRK"
     gene            complement(33850..34515)
                     /locus_tag="SCJ86_000832"
     CDS             complement(33850..34515)
                     /locus_tag="SCJ86_000832"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296524.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HEG1168413.1"
                     /translation="MELDFSSPYYVVVGQLSDNQQLCQERINPLLELLERVFSYYGQA
                     LLTVHRQQIIILVNRISRASLLSLLDKIQTKFNKMYQLQLNFGIGSLCYTEQETPQSF
                     LHAKQVCEWIAFHQSVNEIRFFEDLDLGIVLPAIPSDQRTLYVKRILKSLTEEEVHLF
                     KKTLACFSKNNGSIKNCSEELFIHKNTLQYRLNKFHSLTGYTPRNYDDYHILKLAFLL
                     VQT"
     gene            34491..34664
                     /locus_tag="SCJ86_000833"
     CDS             34491..34664
                     /locus_tag="SCJ86_000833"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296523.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEG1168414.1"
                     /translation="MEKKNQAQLSEGGIDYVLDIINEPMVLSYAMEQVERLLVCAGKQ
                     AMRLIRLGEKIRR"
     gene            complement(34709..35446)
                     /locus_tag="SCJ86_000834"
     CDS             complement(34709..35446)
                     /locus_tag="SCJ86_000834"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010760672.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter ATP-binding protein"
                     /protein_id="HEG1168415.1"
                     /translation="MAEKILVEHLVKKYGDNTVLNDINVSINEGDVVCIIGPSGSGKS
                     TFLRCLNRLEEPSDGEIIIDGAHLMDKSTDINLVRQHIGMVFQHFNLFPHLSILENII
                     LAPMDLKKESREEAEKKAIKLLQTVGLEDKKDMYPEMLSGGQKQRVAIARALAMNPDI
                     MLFDEPTSALDPEMVGDVLNVMKKLAEQGMTMVIVTHEMGFAKEVANRVMFIDDGNFL
                     EDGTPQQIFENPQNERTKDFLNKVLNI"
     gene            complement(35448..36950)
                     /locus_tag="SCJ86_000835"
     CDS             complement(35448..36950)
                     /locus_tag="SCJ86_000835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294446.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter substrate-binding
                     protein/permease"
                     /protein_id="HEG1168416.1"
                     /translation="MKRKHLFHSFVFLMTGLVTFAFGHVAQADNTTTAESSTSETMQK
                     IEPKKDVYVIASDSAFAPFEFQNAKGEYEGIDVKLMENIAKLQGFNIRMDYRGFSAAL
                     QALESGQADGMIAGMTVTDERKKSYDFSDSYFDSGIQLAVKKGDTSIKSYEDLKGKTV
                     GAKVGTESAEFLSDHESEYGYSIKYFDAADQLYDGLKVGAIDAMMDDYPVIGYAIKQG
                     QELETPIKKESGGKYAFAVKKGQNPELLNMFNEGLKELKRTGEYDKIIKEYVADGSET
                     SSESSTADESTFLGLIQNNYQALLKGLWRTILLTLISFALAIVIGVIFGLFSVAPIRA
                     LRTIASIYVDIIRGIPMMVLAFFIFFGLPGIVGFTIPDFLAGVITLTLNASAYIAEIV
                     RGGINAVPVGQMEASRSLGLSYNRTMQKIILPQAIRIMIPSFVNQFVISLKDTTIISA
                     IGVVELLQTGKIIVARTTQSTYVYLIIAIMYLILITALTKLAKVLEKKVK"
     gene            37209..39203
                     /gene="uvrB"
                     /locus_tag="SCJ86_000836"
     CDS             37209..39203
                     /gene="uvrB"
                     /locus_tag="SCJ86_000836"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300378.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrB"
                     /protein_id="HEG1168417.1"
                     /translation="MIERDTSRHFELVSKYQPAGDQPEAINQLVDGVVGGKKAQILLG
                     ATGTGKTYTISNLIEKVNKPTLIIAHNKTLAGQLYGEFKEFFPNNAVEYFVSYYDYYQ
                     PEAYVPSSDTYIEKDSSVNDEIDKLRHSATSSLLERNDVIVIASVSCIFGLGSPFEYQ
                     KQVVSIRQGAELDRNQLIRDLVSIQFERNDIDFQRGRFRVRGDVVEIFPASRDERALR
                     VEFFGDEVERIREVNALTGEVLGETEHVAIFPATHFVTNDEHMEHAVANIKAELEQRL
                     TVLRNENKLLEAQRLEQRTNYDIEMMLEMGYTSGIENYSRHMDGRKEGEPPYTLLDFF
                     PEDFLIVADESHVTMPQIRGMYNGDRARKQMLVDYGFRLPSALDNRPLRLEEFEKHVN
                     QIIYVSATPGPYEHEQTDTVIQQIIRPTGLLDPVIEVRPIMGQIDDLVGEINERVEKD
                     QRVFVTTLTKKMAEDLTDYFKELGIKVKYLHSDIKTLERTEIIRDLRLGEFDVLVGIN
                     LLREGLDVPEVSLVAILDADKEGFLRSERSLVQTIGRAARNEEGKVIMYADKVTDSMR
                     LAMDETSRRRTIQQKYNEEHGIVPKTIIKEIRDLISITKESEDDTKEAVQVSYEEMTK
                     EEKDTLLMKLEKEMKDAAKALDFETAANVRDMILELKASK"
     gene            39213..42029
                     /gene="uvrA"
                     /locus_tag="SCJ86_000837"
     CDS             39213..42029
                     /gene="uvrA"
                     /locus_tag="SCJ86_000837"
                     /EC_number="3.1.25.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629327.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrA"
                     /protein_id="HEG1168418.1"
                     /translation="MANDKITIHGARAHNLKNIDVTIPRDKFVVVTGLSGSGKSSLAF
                     DTLYAEGQRRYVESLSAYARQFLGQMDKPDVDSIDGLSPAISIDQKTTSKNPRSTVGT
                     VTEINDYLRLLFARVGHPICPNDHIEITSQSVDQMADQVMALPERTKIQVLAPIVVKK
                     KGQHKKIFERIQKEGYVRVRVDGEIYDLSEVPELEKNKKHDIAIVIDRIIVKEGIRSR
                     LFDSLEAALRLADGYALVDVIGEEEMLFSEHYACPYCGFTVGELEPRLFSFNAPFGAC
                     PDCDGLGVKLEVDQDLVIPDRTKTLSQGAIVPWNPISSQYYPQMLAQACESFGIDLDT
                     PFEELPEEHQEIILNGTDDLFHFHYENDFGGVRDVETTFEGVLKNIERRYHDTNSDFT
                     REQMRLYMTELTCQTCKGYRLNPQALSVQINGTNIGQTNELSIQKAVDFFSNLTLSEQ
                     EKVIAKPILKEVNDRLSFLENVGLDYLTLSRASGTLSGGEAQRIRLATQIGSNLSGVL
                     YILDEPSIGLHQRDNDRLIGSLKKMRDLGNTLVVVEHDEDTMWAADYLIDVGPGAGEQ
                     GGQIVAAGTPEEVANDENSLTGKYLSGKKSIPVPKERRKGNGKAIKITGASENNLKNI
                     SVEFPLGEFVAVTGVSGSGKSTLVNSILKKSLAQKLNKNSAKPGKFKTISGYESIEKI
                     IDIDQSPIGRTPRSNPATYTSVFDDIRGLFAQTNEAKMRGYKKGRFSFNVKGGRCEAC
                     RGDGIIKIEMHFLPDVYVPCEVCHGKRYNSETLEVHYKGKSIADILEMTVEDAVEFFK
                     HIPKIHRKLQTIVDVGLGYVTMGQPATTLSGGEAQRMKLASELHKISNGKNFYILDEP
                     TTGLHSDDIARLLHVLQRLVDAGNTVLVIEHNLDVIKTADYIIDLGPEGGEGGGTILA
                     TGTPEEIINVKESYTGHYLKKIMV"
     gene            42292..43557
                     /locus_tag="SCJ86_000838"
     CDS             42292..43557
                     /locus_tag="SCJ86_000838"
                     /EC_number="6.3.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294441.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-aspartate ligase"
                     /protein_id="HEG1168419.1"
                     /translation="MMNSIENEEFIPILLGSDMNVYGMARSFNEAYGKICQAYASDQL
                     APTRYSKIVNVEVIPGFDKDPVFIETMLRLAKERYSDKSKKYLLIACGDGYAELISQH
                     KQELSEYFICPYIDYSLFERLINKVSFYEVCEEYDLPYPKTLIVREEMLVNGHLEQEL
                     PFEFPVALKPANSVEYLSVQFEGRKKAFILETREEFDLILGRIYEAGYKSEMIVQDFI
                     PGDDSNMRVLNAYVDEDHQVRMMCLGHPLLEDPTPASIGNYVVIMPDYNEKIYQTIKA
                     FLEKIEYTGFANFDMKYDPRDGEYKLFEINLRQGRSSFFVTLNGLNLARFVTEDRVFN
                     KPFVETTYGTNQSDKARLWMGVPKKIFLEYARENEDKKLAEQMIKENRYGTTVFYEKD
                     RSIKRWLLMKYMFHNYIPRFKKYFHVKEG"
     gene            43561..44292
                     /locus_tag="SCJ86_000839"
     CDS             43561..44292
                     /locus_tag="SCJ86_000839"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296517.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate racemase"
                     /protein_id="HEG1168420.1"
                     /translation="MENFFSILGGMGTMATESFVRLINHRTKATKDQEYLNYVLFNHA
                     TVPDRTAYILDRSEENPMPFLLDDIEKQNLLRPNFIVLTCNTAHYFFEELQAATDIPI
                     LHMPREAANELVRQHTTGRVAILGTEGSMKAGIYEREVKNLGFETVIPDTALQEKINY
                     LIYHEIKESDYLNQELYYEILEEAVERLNCEKVILGCTELSLMHEFAEDNHYPVIDAQ
                     SILADRTIERALAERSEALDTASEK"
     gene            44427..45311
                     /gene="rapZ"
                     /locus_tag="SCJ86_000840"
     CDS             44427..45311
                     /gene="rapZ"
                     /locus_tag="SCJ86_000840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317628.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNase adapter RapZ"
                     /protein_id="HEG1168421.1"
                     /translation="MTDNLQLVIITGMSGAGKTVAIQSFEDMGYFCIDNMPPRLIPKF
                     WELIKESGKVTKMALGVDLRSRSFFEEIQNMLIEIENTNFIDTSILFLDASDEELVAR
                     YKETRRAHPMAMDGLITEGIRKERAILDDLKAQASVIIDTTTLTPRQLREKINESFKS
                     KGDQGFRIEFVSFGFKYGLPIDSDIVMDVRFLPNPHYIPELRPLTGKDQPVYDYVMSF
                     DETQSFYHKFLDLLETVLPGYIKEGKSSLTVAIGCTGGQHRSVALTERLANALGKNYK
                     VNVTHRDKDKRKETVNRS"
     gene            45308..46306
                     /locus_tag="SCJ86_000841"
     CDS             45308..46306
                     /locus_tag="SCJ86_000841"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289697.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YvcK family protein"
                     /protein_id="HEG1168422.1"
                     /translation="MKMKTYRIRKPKIVVVGGGTGLPVILKSLRNQGADITAVVTVAD
                     DGGSSGAIRESIAMAPPGDLRNVLVALSDMPQFYEDIFQYRFKKEDQFLANHTIGNLI
                     IAAVSEMRGSTYEAIQLLAKMMHVDGHVYPSSETPLTLHAVFKDGTTAVGESKIAINR
                     KTIDRVYVCNTNDKSEAKAARKVVSAIMDADMVVLGPGSLFTSILPNLVIPEIGEAML
                     KTTAETVYICNIMTQKGETEHFTDADHIRVLHKHLNQPFIDTVLVNTEKVPEDYMDPE
                     IYDEYLVQVAHDFNGLRNEGCRVISTDFLELKDGGVFHDGDKVVEELFRLVFGSKY"
     gene            46340..47275
                     /gene="whiA"
                     /locus_tag="SCJ86_000842"
     CDS             46340..47275
                     /gene="whiA"
                     /locus_tag="SCJ86_000842"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010752056.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein WhiA"
                     /protein_id="HEG1168423.1"
                     /translation="MSFAADVKKELTGLEVHREHAKAELAALLRMNGSLGIVNQQFIL
                     NVQTENAAIARRIYSLLKDHYQVRSELLVRKKMKLKKNNVYIVRLKQGTKDVLMDLDI
                     MDGMMFNGHVSDEIMGNSQKMRSYLRGAFMASGSVNNPETSRYHLEIFSIYEQHNQDI
                     CDMLNYYGLHARTLERRNGYISYLKGAEKIADFLTLIGATNSMLRFEDVRIVRDMRNS
                     VNRLVNCETANLNKTIDAASKQIENIELIEAKVGLHALPEKLQEIAELRLQHPEVSLK
                     ELGEMIPSGAISKSGINHRIRKINEFAEKLREEAV"
     gene            47818..48468
                     /locus_tag="SCJ86_000843"
     CDS             47818..48468
                     /locus_tag="SCJ86_000843"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321528.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEG1168424.1"
                     /translation="MFIKGGQKMCFFTTFTIIMIAVLFIFLDIAKRNTAFLLYRVLLR
                     AGLITFISIVGFFLFTVIVFIWRTTAPPLPEITYGEFPFSLEYEIDEERILIEDTVIA
                     EFIGSFRGNVTTQPRRSWSIKLLNNSGELKAFHYEFLIIESEDVTITLSSGSAGYFMN
                     DGGSKPQLTVRDRIAGFMHFSHYDLEEARKILAEYGITLINWEYAPPIRNFFNDYY"
     gene            48655..>49215
                     /locus_tag="SCJ86_000844"
     CDS             48655..>49215
                     /locus_tag="SCJ86_000844"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326695.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF443 family protein"
                     /protein_id="HEG1168425.1"
                     /translation="METVRLWIFMKNFRSALRYVILESKSGYYLIDTDHYTFWGGYLF
                     PIFNWIIPHKVMKINLPAEEINRLILNKDLQRKREKEHSNVAWIGPAFAVVTSSIVGP
                     MLESIYSDFSIGIRYLFVTLSIAVLLCIKGYVSKKNKDILTLIGVNNLSSTKIILFPE
                     SIKEGSRLLIIFIMLILFSVLFGVMFI"
BASE COUNT        16475 a         8422 c        10576 g        13742 t
ORIGIN      
        1 tgtttttatt tatatttctt ttatttaaaa attaaaaaaa gaaaaaatta ttcagttttt
       61 aaataaaaaa acaatgattt cataggaaaa acagttagat attgctataa tacttaagat
      121 agtgggaatc ttttaagtat aggaggaaaa agaatgttac gtttttttcc gcaaaaaaac
      181 aaatatattc aagaaaaaag cgaatttcaa tttaataccg atggaccaat tcctcagcac
      241 attgccatca tcatggacgg caatggtcgt tgggcgcaaa accgtcgttt gccacgagtt
      301 gctggccata aagaaggcat ggaaacagta aagaaagtca caaaaaaagc atcaaggctt
      361 ggagtgaaag tgttgacgct ttatgccttt tcgactgaaa actggaagcg accaaaagat
      421 gaagtaagtt ttctcatgca actacctgtc gacttctttg atacgttcgt accagaattg
      481 attaaagaaa atgtcaaagt tcatgtcatg ggttatgaaa acgtattgcc agaacatacg
      541 caagatgctg taagacgtgc aattgagcaa acaaaaaaca atactggaat ggtattgaat
      601 tttgctttga attatggaag cagagcagag atcgtgactg ctgtccaaga aattgccgaa
      661 gaagtagcaa aaggagaaat acacgcagaa gaaatcgatg atgaattgat tgctaagcat
      721 ttaatgacag gtttcttgcc taaagagtta caagatcctg agctgatgat ccgtacaagt
      781 ggagaagagc gaattagtaa ttttctgtta tggcagatcg catatagtga gctgtatttt
      841 acaaaagcgc tttggcctga ttttgatggt gcgcatcttg aagaagcaat cgcctcttat
      901 caaaatcgag accgtcgatt cggcggtgta aaaaaaacag aagatgagaa aggggatcag
      961 tcatgaaaca acgtgtggtc acagcagccg tggcgctagt cttgtttatt ccgattatat
     1021 ggatcggagg catagcggtt gagcttacag cagcaatctt agcagtaata ggagtttacg
     1081 agctgtttcg gatgaaagga ttgacactct taagttttga aggagtttta tccgccgttg
     1141 gtgcagtgtt acttgttttg ccaaaagaaa gatggttttt ctttcttcca gagaatgtaa
     1201 gtacatttat gttattttat cttatagtta tgattctttt aggaacatca gttatttcta
     1261 aaaatacgta tacgattgat gaagcaggtt ttcctgtgct tgtcagcttg tatgtgggaa
     1321 taggtttcca gaattttgtc aatgcacgaa cagaaagctt ggctgttttg atatttggtc
     1381 tatttattgt ttgggcaact gatatcggag catatatggt tggtcgacgt tatggtcagc
     1441 ataagttaag tcctgatatt tcgcccaaca aaacgattga aggtgcgctc ggtggtattg
     1501 gtagtgctgt tgtagtcgca tttctttatt tattgctata cccagcaaaa gaactattta
     1561 atcatggtat gtttgtcatg cttctgatga cgattatttt ttcagcagtc ggacaatttg
     1621 gtgatttggt cgaatctgca atcaaacgtc attatgatgt gaaagactcg ggaaatatct
     1681 taccaggcca tggtggaatc ttagaccgtt tcgacagttt gctgtttgta tttcctatca
     1741 tgcatttatt tggcattatt tagtaagtat acctgcgttt ctttgtgaat ttcataaaag
     1801 acgcaggttt ttatttttca ttatattgat actattggtg tctttggtat actatggtaa
     1861 aattatagtg ttttatcgtt gttttataaa aaacacaaaa aacaagtaaa ggagaaaaaa
     1921 gctatgaaaa cgattctgac atttatcatc gtttttggta tattagtgat tgttcatgag
     1981 tttggtcatt tcttctttgc gaaacgatca ggaatcctcg ttcgcgaatt tgctatcggt
     2041 atgggtccga aaatttatgg gcatcaagca aaagacggga caacatatac gcttcgccta
     2101 ttacctattg gcggttatgt tcgaatggca ggcaatgggg atgatgaaac agaaatggca
     2161 ccaggaatgc ctctttctct tttactaaat tctgacggca tcgtagagaa gataaattta
     2221 agtaagaaaa tccaattgac aaatgctatc ccgatggaat tgagccgtta tgatttagaa
     2281 gatgagctga ctatcactgg ttatgtgaat ggagacgaaa cagaagtagt aacctaccct
     2341 gttgatcacg atgcgacgat tattgaaaac gacggaacag aaatcagaat tgcgccaaaa
     2401 gacgtacagt tccaatcggc taaattgtgg cagcgtatgc tgaccaactt tgctggaccc
     2461 atgaataact ttattttggc aatcctgtta ttcatcattc tggcatttat gcaaggtggt
     2521 gttcaagtga cgaacactaa tcgcgtagga gagatcatgc ctaatggagc tgcggcagaa
     2581 gcaggcttaa aagaaaatga tgaggtagtc agtgtggacg gaaaagaaat ccattcatgg
     2641 aatgatctga caacagtcat tacgaaaaat ccggataaaa cattagactt taagatcgaa
     2701 cgagaaggtc aggtgcaatc tgtcgacgtg acaccgaaat ctgtagagtc aaatggcgaa
     2761 aaagttggcc aacttggtat aaaagcccca atgaatacag gttttatgga taagatcatt
     2821 ggagggacgc gtcaagcttt cagcggttca ttggaaattt tcaaagcctt aggatcacta
     2881 tttacaggtt tcagtttaga taaattaggc ggacctgtca tgatgtacca attgtcttca
     2941 gaagcagcaa atcaaggtgt aacaactgtt attagtttga tggcgcttct ttcaatgaat
     3001 ctcggaatcg tcaatctgct tccgattcct gccttagatg gcgggaaatt agtcttgaat
     3061 atttttgaag ggattcgcgg gaaaccatta agtcaagaaa aagaaggaat attgacatta
     3121 gctggctttg gatttttaat gttgttgatg gtgctagtta catggaacga tattcaacga
     3181 ttctttttct agaaaaagtt gacaaatagg gtataataac aaacgataac tattttgagg
     3241 aaataaagga gtatatagaa tgagacaatc aaaaatgttg attccaacat tacgcgaagt
     3301 tccaaatgat gcagaagtat taagccatca aatcttgcta agagctggat atatccgcca
     3361 agtatcagcg ggtatttatt catatctgcc attagcaaat cgtgtattgg aaaaactgaa
     3421 acgcattatg cgggaagaat ttgaaaaaat cggagcggta gaaatgctga tgcctgccat
     3481 cttgccagca gaactttggg aagaatccgg gcgttacgaa acatatggac caaacttgta
     3541 tcgtttcaaa gaccgcaatg atcgaaaaat gatccttgga ccaactcacg aagaaacttt
     3601 tactgatttg atgcgtaatg aaatcaattc atataagaaa ttacctttaa atctttatca
     3661 aatccaacct aaatatcgtg acgaaaaacg acctcgcttc ggtttgcttc gcggaagaga
     3721 gtttatcatg aaagatgcgt attctttcca tgcaacggaa gaaagtttgg acgagacata
     3781 taaagattac gaaaaagcct acaccgaagt attcaagcgt tgtggcttag aatttcgctc
     3841 gattatcgga gacggtggcg caatgggcgg gaaagactca aaagaattca tggcgatctc
     3901 agaaatcggg gaggatacga tctgttattc gactgaaagt gattatgctg caaacttgga
     3961 gatggctaca agctactatg tatcaaaaaa atcgcatgaa acacagcttg aattggagaa
     4021 agtagctaca cctaacgtaa aatcaattga cgaagtagca gatttctttt ctgttgaacc
     4081 tcaaaaaatc atcaagtctg tattgttcat tgcggatgaa caacctgtcc ttgttttggt
     4141 acgcggagac catgaagtca atgacgtaaa actgaaaaac tttttatctg ctgacttttt
     4201 agaagaagca acagaagaag aagcacaaaa atatctaggt gctgagtttg gttctgtcgg
     4261 accagtcggt gtttcagatg acgtaaaagt atatgcagac cgttatgtcc aagatttagc
     4321 gaatgccatt actggagcga acgaaacagg attccactat aaaaatgtga atccagaacg
     4381 cgatttcact gtactttctt atgaagatct tcgttttgta caagaaggag atccttcacc
     4441 agacaacaat ggtgttcttg cctttactcg aggaatcgaa atcggacata tcttcaaaat
     4501 cggcactcgt tacagtgaat ctatgggagc aactgtacta gacgaaaatg gacgagaaaa
     4561 atttgtgatt atgggctgtt atggtatcgg tgtaagtcgt ttattatcag caatcgtaga
     4621 acaacattca gatgaacaag gaatcaactg gccaaaagga attgcgccat ttgatttgca
     4681 tatcgtccaa atgaatctaa aagacgaata ccaaacaaac ctgacgaatg aactagaaga
     4741 agcgatgaca aaagctggct atgaagtact ggttgatgac agaaatgaac gcgcaggagt
     4801 gaaattcgcg gattctgatt tgattggctg tccaatcaga atcactgtcg gtaaaaaagc
     4861 agtggatgga atcgtagaag tgaaaatcaa gaaaactggc gaaatggttg aagtacgtaa
     4921 agaagaattg gtgaatactt taccaatcct cttgaaccaa ggcgaataat aagcgttaaa
     4981 acacagaaaa taaaaatagc tgaatcagct gtttttgaca atctgatgat tcgcttttaa
     5041 aattggaact gcgatttgaa tcgcagttcc aattttttaa tgtaaaactc agaaaaaacg
     5101 agtatactaa aaggtactga aaaagaagag gataagatgc gatcaattgc attgtttctc
     5161 atcagaaagg gcgtatatct ttgtcagaaa aacgagattt atttgaaaaa ctgttggaac
     5221 aaatccaact agaggaagca gaaaaaaatc atccactgct tactgctggt gaaatggata
     5281 gtgtagtggt ccatcgtaaa tcgcgtttat gggaatttac gctgcatttt tccaaaattt
     5341 tgcctattat gctatatcgg tcattgactc agcatctgac tatcgctttt aaagatattg
     5401 cacaagtcaa actgaatatc ttagcagatg accagacatt cgatgaacag ctcttgcaag
     5461 attactgggc ccaagctttg gaaaatcagc aatgcgatac accactagca cagcaagtat
     5521 tgaaaacgca ggtacctgtg ataaaagatc gtaaagtcat cttgccaatc gacagtacag
     5581 gagctatttc ttatttgaag caacaatact tgccattagt agaagagttg ttcgtcagtt
     5641 acggattccc aaaatttcgt atcgaaccag aagtggatga gcagcaagcg gaacgagtat
     5701 tgaaactttt tgaagaacgc aaacaagaac aggcagaagc ctttatgaag caagcagctg
     5761 aaagtctagt tgtccacgaa caaaagaaaa aggaaagaaa agagcaatcg ccagctcttg
     5821 aaggacccat ccacatccag ctaggacgaa atattccagc cgatgaacca acgacaccaa
     5881 tgatcagtat cgttgaagaa gaacgaagag tcacacttga ggggtacgta ttcgacaaag
     5941 aagtcagaga actacgttca aaacgcaaga tcttgacttt gaaaatcaca gattatactt
     6001 catcatttat cgtcaaaaaa ttctcgaacg gcgaaaaaga tgaacaagta tttgatgcga
     6061 tcagtgtcgg tagctggtta aaggtacgag gaagtattca agaagatacg tttgtccgtg
     6121 atttggtcat gaatgcgcaa gatatcatag aagtcaaaca tacacctcgc aaagattatg
     6181 ctcctgaagg ggaaaaacga gtagagcttc atgttcatag caatatgagt acgatggatg
     6241 caacaaacag catctctgat cttgtggcac aagcagggaa atggggacac cgagctatcg
     6301 ccattacaga ccacggtggt gcgcaagcct ttccggaagc acatagtgct ggaaaaaaag
     6361 caggtgtgaa aatcttatac ggcgtggaag cgaatgtcgt agatgatggt gtcccaattg
     6421 cttataatga tgcacatgaa gcattgagtg aagccacata tgtcgtattt gacgtggaaa
     6481 cgacaggatt gtctgcagta tatgacacga tcatcgagct agccgcagtc aaaatgtata
     6541 aaggaaatgt gatcgaaagc tttgatgaat ttatcgatcc aggacatcct ttgtcacgaa
     6601 caacggtaga tctgactggt atcacggatg gaatggttcg aggatcaaag tcggaagaag
     6661 aagtattgcg gatgttcctt gaattttcaa aagatacaat tttggtagcc cacaacgcgg
     6721 cttttgatat ggggttcttg aatactagct acgcaaggta tggtattcct gaagcggcca
     6781 atccagtcat tgatacgttg gaattagcta gatatctgta tcctcaattc aaacgtttcg
     6841 gattaggtgt gttatctaaa aaatttggtg tcagcttgga acagcatcac cgagcaatct
     6901 atgatgctga agcaactggt cacttagcat ggatctttgt gaaagaggca atggacaatc
     6961 ataacatgct ttatcatgat cagctaaatg agcatatcgg agaaggagat tcttacaaaa
     7021 gggctcgacc tttccatgta acgattttag caaaaaatca ggcaggactg aaagatttgt
     7081 tcaaactgat ttcaatgtcg aatgtcgaat acttcgaacg cgtcccacga ataccacgct
     7141 cccaattgaa aaagatgcgt gaaaatctat tgatcggctc agcttgtgat aagggagaaa
     7201 tattcgaagc catgatgcaa aaaggggtag aagaggcaag aaaccgagca aaattctacg
     7261 attatattga agttatgcca aaagcggtct atgcgccttt gatcgagcaa gaattggtaa
     7321 aaaatgaaca tgatttggaa gaaattatcc aaaatttggt agaaatcgga aaaagtttgg
     7381 ataaaatcgt tgttgcaaca ggtaatgtac attatctgaa cgaagaagat gccatttacc
     7441 gaaaaatttt gatcaattct atgggcggag cgaatccgtt gaatcgtcat agtttgccgg
     7501 atgtccattt ccgaacaacc gatgagatgc taactgcatt tcacttttta ggggaagaaa
     7561 cagcgaaaga gatcgtagta gaaaatacga ataaaatcgc agatatctgc gaagaagtga
     7621 ttccagtcaa agatgaactt tatacaccga agattccagg ttcagaagat gaaatatcag
     7681 aattgagtta cacaaaagca aaacaaatgt acggagatcc gttacctgag atcattcaaa
     7741 aacgattaaa aaaagagttg aattctatta atggtaatgg cttctcggtt atttatctga
     7801 tcgcccaaaa actagtgcat aaaagtaatg aagatggcta tctagtaggt tcacgtggct
     7861 ctgttggctc tagctttgtt gcgacgatga caggaatcac tgaagttaat ccgttagccc
     7921 ctcattatta ttgtccagag tgccaatatt ccgagttttt tgaagatggt acgtatggtt
     7981 ctggatttga catgccagaa aaacaatgtc caaaatgtgg tgcacgctta aacaaagacg
     8041 gtcatgatat tccatttgag acatttttag gattccatgg agacaaagtg cccgatattg
     8101 atttgaactt ctcaggtgat tatcaagcag aagcccataa ctatacgaaa gtattgtttg
     8161 gcgaagacta tgtttaccgt gccggaacga ttggtacagt cgccgacaaa acagcatatg
     8221 gctatgtaaa aggatacgaa agggacaata atctgcagtt tcgaagtgca gaagtcgaca
     8281 gattagcaaa aggcgctact ggagtcaaac ggacaaccgg ccagcatcca ggggggatca
     8341 tcgttattcc cgattatatg gatgtctatg attttacgcc gatccaatat ccagcagacg
     8401 atcaaaattc ggaatggaaa acgactcatt ttgattttca ctcgattcac gataatgtat
     8461 tgaaattaga tattctaggg catgatgacc caactgttat tcggatgctt caagatttat
     8521 cagggatcga tccacaaacg atccctacgg atgacccgga agtgatgcgg atatttgccg
     8581 gtcctgaagt acttggagta agtcaggaac aaatttattc caaaacaggg acattaggta
     8641 ttccagaatt cggcactcgt tttgttcgag ggatgctaga agaaacgcat cctacaactt
     8701 ttgcagaact actgcaaatt tccggactat ctcatggtac agacgtatgg ttaggaaatg
     8761 cggaagaatt gattcgacga ggagatgcga cactggcgga agtaatcggc tgtcgtgatg
     8821 atattatggt ttatttgatc catgctggat tagatagcgg gatggcattt aaaatcatgg
     8881 aaactgttcg taaaggacaa tggaacaaga tccctgatga attgcgagaa acctatctat
     8941 cggcgatgaa agaaaataat gtgcccgact ggtatatcga ttcttgttca aaaatcaaat
     9001 atatgtttcc gaaagcccat gctgcagctt acgtattgat ggctcttcgt gtcgcttatt
     9061 tcaaggttta tttccctatt ttgtattact gtgcatactt ttctgtacgt gcagacgact
     9121 ttgatctagt ctccatgtgc aaaggaaaag atgctgtgaa acaagcaatg aaagaaatca
     9181 cggacaaagg attagatgct tccgtaaaag aaaagaatca actgacagtg ctggagttag
     9241 ccaatgaaat gctggaacga ggattcaaat ttggaatgat tgatttgtat aaatcggatg
     9301 ccgttaactt tgttattgaa ggtgatacgt tgatcgcacc atttagagct gtcccaagtt
     9361 tgggaaccaa cgtggcaaaa caaatcgttg aagcaagaaa agacggtcca tttttatcca
     9421 aagaagatct tgccacccgc ggtaaagttt caaaaacatt gatcgaatat atgaacgaca
     9481 atggcgtatt gaaagatcta ccagatgaaa atcaattatc gttattcgat atgctataag
     9541 catctgtttt cctgtctgct tgtaatttta agcagagtat ggtatagttc ttataggtaa
     9601 tgatactaac tgtgagtgag cggatttttc gctcactctt tttaatggaa ttacgcactt
     9661 aattttttaa ggaggcgaaa cttttgagta gcgtcgttga aacagtcaca gaaatggtga
     9721 caccgatttt agatgaacaa aattttgaac ttgtcgaagt cgaatttgta aaagaaggaa
     9781 aaagctggtt cttacgagta tttattgaca aagaaggcgg tatcgatatc gaagaatgcg
     9841 cctttgtcag tgagaaactg agtgaaaaac ttgatgcagc agatccagat ccgattcctc
     9901 aagcttattt cctggaggta tcctccccgg gagcagagcg gccattgaaa aaagaaagcg
     9961 actatgaaaa ggcacttggt gattatgtcc atgtctcctt ataccaacca gtagatggcg
    10021 aaaaacaata tgaaggattt cttcaatcat ttgatgctga acagcttaca ttgaaaatac
    10081 gcataaaaac acgagaaaaa gaattgactt ttgaccgaaa aaatattgca aaagctcgct
    10141 tagctatcca attttaatca cggagaggaa tcagaaaaaa tgagtaaaga aatgttaaat
    10201 gcgttagacg ctttagaagc tgaaaaaggg atctcaaaag aaatcgtgat cgatgcttta
    10261 gaagcagcat tggtttctgc atacaaacgt cactatggcc aagcacaaaa cgtcgaagtg
    10321 gaatttgatc agaaaaaagg aaagatccat gtctatgcgg taaaagaagt aacggaagaa
    10381 gtgatggatt cacagttgga agtctcattg aaagatgcac ttttaatcaa tccagcatat
    10441 gagatcggtg atacgatccg ttttgaagta acaccaaaag attttgggcg gatcgctgcg
    10501 caaacggcta aacaagttat tttacagcgt gtacgtgaag cagaaagaac gattatttat
    10561 aacgaattca gtgcttatga aaaagacatc atgcaaggaa tcgtcgaacg ccaggataaa
    10621 cgttatattt atgtcaatct tggtaaaatt gaggcagttc tttctaaaca agatcaaatg
    10681 ccaaatgaat tttatcaacc acatgatcga atcaaagttt atgtttcccg agtagaaaat
    10741 acgtctaaag gtccgcaagt atttgttagc cgcagccatc cagacctatt gcgccgctta
    10801 tttgagcaag aagtaccaga agtctatgat ggaatcgttg aaattgtcag catcgcccgt
    10861 gaagctggcg atcgttcaaa agtggcagtt cgctcaaatg atccaaatat cgatgcagtc
    10921 ggtacatgtg tcgggccaaa gggacaacga gttcaagcaa tcgtcaatga attaaaaggc
    10981 gaaaacatgg atatcgtcga atggaatgaa gatccagctg tattcattgc aaatgcattg
    11041 aatcctgcac aagtcatgga tgtgatcttc gatgagacaa atccaaaggc ctgcacagtt
    11101 gtcgttccag actaccaatt atcgctagca atcggtaaac gcggacaaaa cgcacgttta
    11161 gctgctaaat tgactaacca taaaatcgat atcaaatcag aatctgacat gacggaattt
    11221 tatgaaaatc aagaacaaca aaaagaaaca gaagaactgc atgatgaagc aatcgttcag
    11281 tcagatttaa cagacgatga atatgaaaca attgcattca acaatgaaac ggtcgaagaa
    11341 aaaccagaag cttaacttta gtaaggaggc aaagagatga aacaaaggaa aattccttta
    11401 cgcaaatccg tggtttcagg tgaaatgaaa cctaaaaaag agatggtccg aatcacccgt
    11461 tcaaaagaag gggaagtatc gatcgatcca actggaaaaa tgcctggacg aggagcgtat
    11521 gtctcacttg agccggaaga agtacaacaa gcttgggata aaaaaattct cgatcgagta
    11581 ctcgaaacaa cattaacaga tgagttttat caagaacttc ttgactatgt cactcaccaa
    11641 aaagcacgta aagagttatt tggaaaatga atacagttaa tcggcaaaaa gccatgaatc
    11701 tcatcggtct agcgatgcgc gcgggaaaac taatcacggg tgaagaactg accattgctg
    11761 aaatccgcaa acaaaaagca aaattggtgt ttgtggcaac cgatgcaagc gagaatacaa
    11821 gaaaaaagat aaaagataag agttcatatt atgaagttcc ttgctttgag ctattttctg
    11881 aagcagaaat cacgcagatg atcggaaaac ctcgcaaggt attcggaata atggatgccg
    11941 gatttgcgaa gaagaccaag gagctaattg aaggttagga aggtgattgc atgggcaaca
    12001 aaagaatcta tgaattagca aaagaactca aaaaatctag taaagacgtc gtggaaaaag
    12061 cacaacaact tggcatcgat gtgaaaaacc atatgggtgc gattactagc aatgatgaga
    12121 agaaattgca acaagcgttc aaaaacccaa gcacgaaaca acaagctgca caacaagcaa
    12181 gcagaaaacc agccaatcaa cagccgaacg atcaacgtaa aaaagaaaca acgaacaatc
    12241 aacaaaaaaa taaaaataac cgcagttatc aagatcgcgg tcaagggagc ggtcaagtga
    12301 atcaaggaaa aaaaccaaca acaaaccaaa ataatacggg aaataccagc agccaaaatc
    12361 gtcaaggaac gagccaaggc ggcaatcaaa accgtactaa caataacaac aaaccaaaca
    12421 ataacaatcg cggcaaattt aataataata accgtaatcg ttataataaa aaaggtaaaa
    12481 aaggcaaaca gcaacaatca actaaaccag ctgtaccacc acgcaaattc cgtgaattgc
    12541 cagatgtatt agagtatact gaaggtatga acgtagcaga tattgcgaaa aaaattcatc
    12601 gtgagccagc agaaatcatt aaaaaattat ttatgcttgg tgtcatggtc aatcaaaacc
    12661 aagcactgga taaagatacg attgaactgt tagcgacgga ctatggcatg gaaccacaag
    12721 aaaaagtcca agttgacatt gctgatatcg ataaattctt cgaggcagaa gaagtcgatc
    12781 cagataaatt agtctcacgt ccgccagtcg taacgattat gggacacgta gaccatggga
    12841 aaacaacttt attggataca ttacgccatt cacgtgtaac aagcggcgaa gccggcggaa
    12901 tcacacagca tatcggtgcc taccagatcg atattgatgg taaaccaatc actttcttgg
    12961 atacaccagg acatgcggcc tttacaagta tgcgtgcacg tggagcaagt atcacagata
    13021 tcacgatctt agttgttgct gcagatgatg gcgtcatgcc acaaacagtt gaagcaatca
    13081 accatgccaa agctgcaggc gttccaatca tcgtcgctgt aaataaaatt gataaaccag
    13141 gtgcaaaccc tcaacatgtt atgcaagaat taagtgagta tgagttaatt cctgaagcat
    13201 ggggtggaga aacgatcttt gttgagatct cagcgaaatt cggccaaaac atcgaagaat
    13261 tgctggaaat gatcctgtta gtcgcagaag tagaagattt aaaagcagat ccaacacaac
    13321 gcgcaatcgg aacagtgatc gaagcacgtc ttgataaagg taaaggtcct gtgtcaacct
    13381 tacttgtcca agaaggaaca cttcatgttg gtgacccaat cgttgtcggt aatacttacg
    13441 gacgtgtccg tgtaatgaca aacgatcttg gccgtcgcga taaagaagca ggacctgcaa
    13501 cgccagtaga aatcactggc ttgaatgatg tacctcaagc aggtgaccgt ttcgtagtct
    13561 tcgaagacga aaagacagca cgtgcagcgg gtgaagaacg cgcgaaacgt gcattattag
    13621 aaaaccgtgc tgccacaagc cgtgtgactt tggataactt atttgaaagc ctgaaagaag
    13681 gcgaattgaa agaagttaac gtaatcatca aagcagatgt acaaggttca gcagaagcac
    13741 tggctgcatc attgaagaag attgatgtgg aaggtgtccg tgtaaaaatc gtccattcag
    13801 ctgtcggagc aatcaatgaa agtgatgtaa ccttagcagc agccagcaac gctattatca
    13861 tcggtttcaa cgttcgtcct acaccgcagg caaaagtgca agctgatact gaagaagtcg
    13921 atattcgtct tcaccgtatc atctataaag ccatcgaaga aatcgaaact gcgatgaaag
    13981 ggatgctaga ccctgaattt gaagaaaaaa tcaccggtca aatgatcgtt cgtgaaacct
    14041 tcaaagtttc taaagttggt acgattgcag gtgcctatgt aacagaagga tatattcgta
    14101 gagatagcgg tgtccgtgta atccgtgacg gaatcgtaat ctatgaaggc caacttgcaa
    14161 gtctgaaacg attcaaagat gacgtgaaag aagtcaaaat gggttatgaa tgtggtgcaa
    14221 tgattgaaaa attcaatgac atcaaagtag atgacgtaat tgaaggattt atcatggaag
    14281 aagtcaaaaa tgactaaaaa ctgataaaag gaggccaatc attatggcta actaccgtga
    14341 ccgccgagtc ggtcaagaaa tcttaaaaga agtcaatgat attttgcgca aaaaggtccg
    14401 tgaccctcgt gtggagaacg taacgatcac agatgtgaaa gttacggggg acttacagca
    14461 agcaacgatc tattacagca ttttatctga cttagcatca gataagaaaa cagcacaaga
    14521 tggattgaat aaagcatcag gattgattcg tcgagagtta gggcacaatc taagtattta
    14581 taagacacct gaattgttct ttgaacttga tgaatcagtt gtctatggca accatatcga
    14641 tgaattgctt cgcgacttga acaaagacta atcaaaaact cccgcaaatc ctgtgaaatc
    14701 aggggttgcg ggattttttt gttttcatta agacgtatta cctaagagtt ccatgacttt
    14761 agtcttgcgg cgtgtcagct aaccaaaaaa gagaaaaaga aaagtaaaaa attcgacttt
    14821 cttttttcta gtagtatata aatttttgca tatagataaa gatcaagata ccgataacgg
    14881 aagctagatt gtaatctatt ttagtgacag tcaggtatag ccaagtaact agaagatcag
    14941 tggaacctgc accataaaag tttgagtttg tcggtccgtt gacagttaaa agaaaaatca
    15001 cgttgaagtt attgatattt ccgattacgc cgcttcgatt tggtcacgat ccaaattttg
    15061 gataatccct gtagaaatta gcatagtagc tgggatacca attcacatat tgacaaagat
    15121 aacagagact tttacataaa acggatcaat caggaaagga attgctcgat ccagccccag
    15181 ttttccagca atgtgttaac tggcccagcg tcattcagca aattatgcat gatgaggagc
    15241 gaaatgaact gcggaacagc cattgtgata acgacaagtt ttttggatca atgtaaaaaa
    15301 cgtcagcgaa aggaaaaatt tcgtggtttt ttatacctgt tcatttggct agatataaaa
    15361 tccaaacaaa tgaacagaag tgcttattag gtttcaattg ggagtatctg cgctagaata
    15421 agagtaataa tgaaaaggag tgttatgtat ggattattct agtaaagaat actttgataa
    15481 aatgacggct tggtggcgtg ctgctaatta tctttctgtt gggcaacttt atcttaaaga
    15541 taacccctta ctgagacgta cactgaaacc cgaagatgtc aaaaaacatc caatcggtca
    15601 ttggggaacg attcctggcc aaaattttat ttatgtccat ttgaaccgag tcatcaataa
    15661 atatgatttg aacatgtttt atattgaagg gccaggacac ggcggacaag tcatggtatc
    15721 gaatgcttat ttagatggaa gttacacaga gatctatcca gaagtaacgg aagatgagac
    15781 aggaatgcaa aaattattca aacgattttc atttcctgga ggcattgctt ctcatgcagc
    15841 accagaaaca ccaggttcta tccatgaagg cggagagctt ggatactctc tttcacatgc
    15901 agtcggagct gtattagaca atcctgaagt aatctctgct gtcgtgattg gtgatggtga
    15961 agcagaaacc ggtcctttag ctggaagctg gttctcaaat gtctttatca atccagtgat
    16021 agatggagct gttttaccga tccttcattt aaatggtgca aaaatagcca acccaacgat
    16081 cttggcacgt aaatcagatg gagaattagc aaattacttt aacggacttg gctgggaacc
    16141 tttctttatt gaaggaaatg attcagaaaa attaaatcct gttatggctg aaaaaatgga
    16201 tcaagcaatc gagaaaatta aatccatcca aaaagaagct cgcctgaaga cagcaacaga
    16261 tgtcgtgatg ccaaaatggc cagtattgat cgtacgcaca ccaaaaggat ggaccggtcc
    16321 aaaagaatgg gacggggaac caattgaagg aaccttccga gcacaccaag taccgattcc
    16381 tgtagatcaa gaacatatgg atcatgcaga tgctttactc cgttggctga aatcctatga
    16441 accagaaaaa ttatttgatg cacaaggacg tattttagaa gaaatcagag aaatcgcgcc
    16501 tactggtgac caaagaatgg caaaaaatcc tatcaccaat ggtggaatcg atccaagacc
    16561 gcttattatg ccagattgga aaaaatatac tttgcaattt gaaaaaccag gctccgttaa
    16621 agcagaagat atgaccgaac ttggtaaatt cgttcgcgag atcattgaga aaaatccaga
    16681 aaatttccgt atatttggac cagatgaaac aaaatcaaac cgattgaatc aagttttcaa
    16741 aacgacaaat cgccaatgga tggaaaaaat cgaaccagaa aatgatgaat ggctttcgcc
    16801 atctggtcgc gtcatcgaca gtcaattatc ggaacaccaa gatgaagggt tcttagaagg
    16861 gtacgttctg actggacggc atggtttttt gcaagttacg aatcattctt gcgagtggtt
    16921 gactctatgt tgactcagca cttcaaatgg atgcggaaat cacatgacct ttcttggcga
    16981 aatgattatc cttcgctaaa tctcattgca agctcaacag tcttccagca agatcacaat
    17041 ggatatagcc accaagatcc aggaatattg acacatttgg cagaaaagaa ggcggagttc
    17101 atccgtgaat atctgcctgc ggatgcaaat acattgctcg cagtcatgga taaagcattc
    17161 cgctcgtcag aaaaaatcaa tttgatcatc tcttccaaac atccgcgtgc tcaattttac
    17221 tcagcagaag aagcagccgt cttagtgaat gaaggcttga aaatcattga ttgggcttcg
    17281 acagcaaaag aggaggaacc tgaattggtc attgctgctg caggaacgga atctaactta
    17341 gaagcattag cagctgtaac tttgctgctg gaagaattcc caaaactaaa aattcggttc
    17401 atcaatgtcg tagatttatt gaaattacgt catccaagtc aagatccgcg tgggttgagc
    17461 gatgaagaat ttgatcaata ttttacaaaa gacaaaccga tcttatttgc tttccacggt
    17521 tatgaaacat tggtccgcac gattttcttt gatcgtcata accatcattt aatgattcat
    17581 ggctacaaag aaaatggaga tatcacgaca ccatttgaca tgcgtgtagt aaatgaactt
    17641 gatcgttatc atttggcaaa agacgcagct ttaaaaatca aaggaagtca agcagaagat
    17701 tttgcgaaaa aaatggatca gaaattacaa gaacatcaaa actatattcg tgaaaatggt
    17761 attgatctgc cagaagtatt ggattggaaa tggaaaaatc ttgaccaata aaatgataaa
    17821 atcgatcccg caaaatagaa ttttttacat tgattaagaa actgaaaaaa taaataaaaa
    17881 aaacggaaaa aagactggaa gaatggccag tctttttttt cgtttttggg aagtgaaaac
    17941 caaactattt acctgtaagc cacccataaa tccaatcaaa tggtgcaaag aaaaaatctt
    18001 ccattctatt ccctccttta aaaaagcata acatgatttc tatttacgtg agcatgaatt
    18061 ttgttacgtt tttattgccg aaaaataaaa accgttcatt atgatgtcaa attcgtcagt
    18121 aattgatgca atgtgttata ataattcagt taaatcaaaa atgaaatgga gaaaagacat
    18181 ggacgggtta ttacctttat ggaaagaacg cggaatgaca agtcatgatt gcgtattcaa
    18241 attaagaaaa atcttacata cgaaaaaaat cggacatggg ggtacgcttg acccagacgt
    18301 agacggtgtg ttgcccattt gtattggaaa agccacaaaa gttatcgaat atttgacaga
    18361 ttctgggaaa atctatgtag gcgagataac tctaggtttc agtacaacga cggaagatcg
    18421 atccggagaa cgggtagaaa caaaaccagt agatatgtct ttatctgaaa aaaaaataga
    18481 tgaagtgatg gcttcattta ttggtgagat cacccagata ccaccaatgt actcagcggt
    18541 aaaagtcaac ggtaagcggt tatatgaata tgcccgcaat aatgaaacag tggagcgtcc
    18601 tgttagaaag gctcagattt accgttttat ccggacaagt gaattgacaa gagacgaagt
    18661 gacaggaacc atttcttggc gcttcgaagt agaatgcggg aaaggaacct atgtccgcac
    18721 attagctgta gatacaggca agaaacttgg ttaccctgcg cacatgtctg atctgacccg
    18781 aacagcaagt gccggtatgt cccagaagga tgctctgaca ttagaagaag tagctcaagc
    18841 tgtgaaaaat gggacaatcg atgattgttt gctgccaata gaaaaaggag tggaacggtt
    18901 caaacgagta gatattactg atgaagtatg gcagaaagta aaaaacggta tgagacttga
    18961 ttatactgta tttgcgctcc cagaaatgcc gtcagaagaa attgctttgt tttataaagg
    19021 aaaagttgtc agtatttatc agccaaaccc gaaagaaaaa aacaaattaa aacctagtaa
    19081 agtattgaga aatgaggaat aacgaatgaa tatcatcaag atcagacatc cttaccaatc
    19141 agaacaaatt ccagaagacg atgtcgtttt agtgctggga tttttcgacg gcgtgcatat
    19201 cggacaccag aaagtcataa agacaggtat ggaaattgca caaaaagaag gattgaaatt
    19261 agcattgatg actttcaatc agcatccatc tattgtcttt aaaaagatcg atcctagcag
    19321 cgtgaaatat ttgactagcc ttaagcaaaa agaagaaaaa atggccgctt tgggtataga
    19381 ttatttatat gaagttgatt ttacttcttc ttttgctcat ttagcaccgc aggaatttgt
    19441 tgatcaatac atcgtagggt tacatgcaaa atatgctgtt tccggttttg attatacgta
    19501 tggaccaaaa gacattgcag atgtcgctca ttttcctgaa tatgccaaag gaagattcaa
    19561 aatcgtcaca gtttctaaag aagaagaaca aggtgaaaaa atcagctcta cccgtattcg
    19621 tggattacta gacagcggac aagtaaaaga agcagcaaaa ctattaggtt gcacatatga
    19681 ggtagaaggg gtagttgtcc atggcgatgc acgaggcaga ctactaggat tccctacagc
    19741 aaatgtcaaa gtaaagagca cggttcattt gcctaaagaa ggtgtttatg tgtcggaaat
    19801 aaaaattggg gaaacatggt atccagccat gggttcaatt ggacataatg atacattcgg
    19861 tgaaggacgg gagctgactg tagagttgta tattttggac tttaatcaag atatctatgg
    19921 tgaacatgtc gcggtacgct ggaatcaatt tttaagagat caagtgaaat tttctagtgt
    19981 agaggaattg attgaacaac tcaaggagga tgaacaagca acagctgaat atttttctca
    20041 gcaataaaac attatgcata aaatctcttg tttatgtata actagcaatt acaataaaat
    20101 gggtgataaa taagcccagg aaaggggctg tgacaaaagt cttgtcacag cctttttttc
    20161 caaataaatg gtttttcaga agtagcttct tcggaaataa gccgaaattt cttgaagctg
    20221 accacttctg tcacaacctc tttctttata gaaataaaag gacactagca attcatgaaa
    20281 acgattgcta gagcgtatca tttgctttag actatatatc ttggtgtaca ctcactttaa
    20341 ggcggaaata aaagttatcc gtaataacag gtttaggtat gaaaaaggag tgggtactat
    20401 gtacaaggaa ttagaaaaca aagtcgcagt agtaactggt ggctcaaagg ggattggaac
    20461 agcgattgct caacgattcg ggaaagaaaa aatgaatgta gttgtcaact ataactcgga
    20521 taaagaagga gcagaaaaag cagtcaaagc aatcgaagag gcaggcggaa aagcagtctc
    20581 tgtacaagca gatgtcagtt ctgaagaagg aatacagcgt ttacttgata cggctttaga
    20641 aaattttggg acactggacg tttgggttaa taatgctggg atggaaaatc agcatccaac
    20701 ccatgaactt agccttgaag attgggaaag agtgttaaaa gtcaatttga ctggcgtttt
    20761 tatcggaacg aaagcggctt taaattactt tacagagaat aataaaaaag gaaatatcat
    20821 caatatgtct tctgttcatg aacagatccc atggccaacg tttgctcatt acgctgcttc
    20881 aaaaggcggg gtaaaattat ttaccgaaac ggtagcaatg gagtatgcac cgaagggaat
    20941 cagaatcaat agtataggcc ctggtgctat ccgtacacct attaatgcgg caaaatttga
    21001 tgatcctgaa caaaaagaac tcttggaaag tatgatccca ctaggacgcg taggtgaacc
    21061 aaaagaaata gcggcgacag ctgcatggtt ggcttcagac gaatccagtt atgttacagg
    21121 tattacacta tttgcagatg gtgggatgac tttatatcct tctttccaag gtggaaaagg
    21181 ataagggtga ataaaaatgg gtttattgat tgcattgatc ccggcagttg gctgggggat
    21241 ccagccactg attttgaaaa agattggtgg cagaccaaca aatgagatat taggaactgg
    21301 tattggagcg gtgcttgtcg gcttactcgt tcaattattc gcttctccag ctggtatttc
    21361 agtgactaca tttttgatta gtttactttc tggtgcattt tgggttattg gacaaacagg
    21421 acagtataca tctttcaact tgattggtgt ttctaaaacg atgccaattt cgactgcatt
    21481 acagttagtc ggaacctcgt tgattggtgt cttcgctttt ggagaatggt ctggaagtat
    21541 ggggaaaatc attggagcag cagcaatcat tcttcttgta tttggttctg ctttaacggc
    21601 aatcagtgat gatcctggaa aaaaaggcgg tatcacaaaa ggaattagtc tattagcatc
    21661 aacaagtatt ggttattggg tatacagtgc attgcctaaa ctagttgatg caagcggact
    21721 ttccattttc tttccgcaaa tgatcggtgt ctttttaggg gcagtgattt atgtgatttt
    21781 ccgtaaacca accgcatttt ctgaagacaa gagttggaaa gcaacgatcg ttggagccat
    21841 ctttagtgtg tcagcattgg cttatatttt ttcagcaaat gaaaatgggg ttgctactgc
    21901 atatatcatt acacagctaa acgtagttat ctctacatta ggcggtatgt acatcctgca
    21961 tgaaaataag acaccaaaag aaaaaaaatt aacgattatc ggattattgc tgatcgtagt
    22021 aggaagcatg gtcacggtct ttatttgagg ataaagatgg taaaatttca tttattaaaa
    22081 aaatatttgt ttgaaccaaa cagtaagtac ttataaaaaa atagcgctag aatttccaac
    22141 atctctggaa tttctagcgc tttttaattg tttaagatac aagggaaata tctgtctgga
    22201 tcaataattt ttcattgatc cattgactaa agtttacgac ataattgaag ttaccaccga
    22261 ttagtatctc cgcatgattg tctttataaa gatcgtattg taaaagacgg tcttttttat
    22321 ttgcgtaaac atcgttatgg tactggacta attgttctcg gaattgattg attgctgtaa
    22381 aatcacgata ttgaaggttg aaacgcatcg tgattggtgc atttagcatg aatcgctgca
    22441 tttcttttgc tgcttcctct gcttgatctc cgtataggaa ctcaaaaaga ttacgttcag
    22501 aaaatttcca ttgcccgcca acctttgttc cttttagttt tccttcatga agataatttc
    22561 tgatagaacg tgtagtaaca cctaattgtt cggctaattc atttactgag taaaaatctt
    22621 tcaatgttca ccctcctatg tgaatggttc tactgactta gttgatattt gcttaatcac
    22681 gaaatgtaga aagaacgatt ttaattaagg tcattgtaaa aatcaagtca gaaaattcta
    22741 acttttattt ctctaaagaa attcgttctc tcctaaagtg atattatatt taaatatacc
    22801 tgaaaataat gggcagtatt atgaaagatc actgatattt tctcgtattt tttccttatt
    22861 caaaagtttt gcaggaaaat tgctgaaaaa ataattttac tatgtagaaa attcaaagcc
    22921 attcaatcag tgattttttt cagcacaaga aagactcctt ataaaaatag cagatacttt
    22981 gtcaattaag tgtttaacca gggatgataa aaaggatagg attgaacaaa acctgtataa
    23041 ggtgatgaat agtttcctcg ccagtaaaag gtttattcgt ataaagcttt atcgccaaaa
    23101 tttatcatga ccttttttat cttcaatatt aaatgaaatc aattgttcaa tcaattccca
    23161 gttgattaca cttttccaag gaactttgat gacgttatcc gtatatacgt agcctgcttc
    23221 ttggatattt ttttcaaggg cacgaatcgc tgctttttcc ggtgcgatag aaaaatgatt
    23281 tttagcaaaa ctaaatgcaa taataaaggt accatggtca gtaaacatag gttggttcca
    23341 ttttacagtt gtgtctagct ggggatacgt ttctgttatc ttagcaaaaa ggtctttgat
    23401 cttttgttga tgttcttttt ctttgatttg attgatgatc ggctgaaatt tttccattct
    23461 aaaacgcctc cattaagtgg tttgatttat tttaccagaa aaacaatagt tatgtataaa
    23521 aaacgaacaa aacacaagta atgttgtaac cgctatattt atatgataag atgtctcaaa
    23581 taataaaata gaaaagagac ataaaaatga ttgacttggc aaagaaattc ttacacgaaa
    23641 atatatattt tgagttgtat catcatcgag ccatttatac aaatgaagat gctttggtaa
    23701 taaagaaaga acaaggattt acaggtacag agaccaaaag cttattctta aaagataaaa
    23761 aagataataa ctacgtatat ctgactttta ctacgaaaca aacggatttc aaagcattag
    23821 ggaagatcgt cggtaaaagg ctttctattg tttcatcaga acgcatggaa gaacaaacgg
    23881 gacaaaagca gggcgctgtt tctccgtttg gttatgaaaa acagattccg attattattg
    23941 atgaagagct cttatcaaat gaaaaacttg tttttgcacc aggtagacca gatcaaacga
    24001 tggtcatcca aacggttgac ttaaacaaaa tcatccagct acttgagatg ccgatatttt
    24061 tgatgccggc aaacgaaaaa aacaattaac caagtaaaat agtgcatact tggaggtgaa
    24121 gcaacatgat aagttatcca ttattagaaa gaaatgcaac gattggggtt actgcaccat
    24181 cctcaggcgt atcagaggaa caccattcgt tactcgaatc agctttaaag agaatggaag
    24241 aaaaatcttt ccaagtagtt actggaaaga cgctttggac gcaagataag gaaaaatcag
    24301 gtccagcaag aaaacgagca gatgaattca atcaaatgat gcaagatgaa aatatccaaa
    24361 tgatctttcc gacttgggga ggagaattat tgattgagat tctcgatttt gtcgattttg
    24421 atgctctaca accaaaatgg atagtaggtt attcagatat tagtacgttg ctacttgctg
    24481 tcacattaaa aacagggata gccactgcac atggaactaa ccttattgat ttaagaggag
    24541 agtacttgga tgaaacgaca gcaatgtggg aaagtgtact gaaaacaaga gaaggagaat
    24601 cgattctaca gcaatcttct gaaaagtatc aaaaagtgtg gccccatgag cattcatcat
    24661 cttctgtttt ccatctcact gaaccaacaa attggcaatc agtcagcgga gatgcagtaa
    24721 ggatgaaggg acgattatta ggtggttgta ttgatacgat caggcattta atcggtactc
    24781 cttatggaga tgtccaatct tttaaaagaa agtataatga tggaaagccg atcttgtggt
    24841 ttctagaaaa ctgtgagcta tcgtcagttg atttaagaag atcacttgtt cagatgaaac
    24901 tggcaggctg gtttgatggc agctcgggga tcatgttcgg tagaagtgcg gcagaggatt
    24961 tgttagatta taaaatgaag gatgtatacg aagatctgtc aaaggaatta aaactaccga
    25021 tcttgtatga tattgattgt ggtcatgtac cgccacaact gacattgatt aatggtgcct
    25081 atgcagaagt agcggtagaa gatagaaaag cgactgtttt gcaaacgttt atttaaaaaa
    25141 ttttttcctg ccttggagtc tatcaaagac caataaatct aaatagaaaa gaaagtgaaa
    25201 gaagctgaca aaatgctgtc agcttctttt tgtataacat attagaaagt ctaattatct
    25261 ataactgtca catcgattaa ctgtttcacg tcattgggat ttcctgtagg aatagctact
    25321 acagatgcat tatcaatgat ttcttgagtt gtttctctaa tgaacgctgg ataagatgtt
    25381 tgatctgcag gcatttgcca atgtgaagga actggataac tagtgattgt cccgtcatgg
    25441 ttacttgaat acactaaaac tccctggtaa ctgtatttcc cagtgagcat aaccacatct
    25501 ttcggataga ctgcactccc tgcatcataa ggggcgatag gtgttcctgc aggataatga
    25561 ataacatgta attcgaaatc acccgatgta tctctaaaag tattacctct aatagctagc
    25621 caaacacgtg cgtactcaat ttgtagatca gaatatcctg ctaacggatc tattgactga
    25681 gaagactgtg aggaggttgt gtctattgtt gaagaagaaa tatttgtctc aactgtacta
    25741 ctagatgtag aagaagtatt gggctctgag actttactgc ttgttgaaga tgtattcgtt
    25801 tcagtagaag ctgatttctg agtacttgat ttttcagctg aactggtttg tctgattgaa
    25861 cttttgtctg tggtcgcaga tggatcacta ttttctttac taggagacgt acatcctaca
    25921 aggaataaag agaccaacaa taaaataaaa cctaactttt tcatataaaa tacctcatta
    25981 tttttttaat accagtatag cataatcctg aatttttgaa tccgtaaaac cttgtctatg
    26041 aatataaata gagtgtatct tctttgataa atgtcagaaa aagtgtgaca aataatcatc
    26101 agtaaaatta cttgtcacac tattgataag gagtatttaa ttgaagagaa tgattaggag
    26161 aaaggatctc cgtacttttt atagtaatca tagaatccaa gtttgtatag acgaatcgtt
    26221 ttttctctta tttcttctgt ttcaaaatgg actgcttgaa gaatcaagtc tgtgaattct
    26281 ataggagctg ttccattcgc agtaatcaaa ttacgatctt tcacagcttg ttgttctata
    26341 aaatcttctt tattctgata attttccgat tctaaccaag gaaattgcgc atttccggtg
    26401 tgtttgtaac cttgcaataa tccgctctga gctaaataat caactgcccc acaaatagca
    26461 gctactggta cgtctgtttt taacaattta tcgattattt gatataaatg atgatttttt
    26521 atgtcccatg aattgccacc gatcataata aaaaggtcaa ctttttcagg tatttgttcg
    26581 attaagtaat cgatttttgt ttttaatcct ccaatggaag tgacctctga ttggatagaa
    26641 gcagttttgt tttgccattt ctcattttgg tttagtgtgc tagtaatata agcaccttcc
    26701 caatcggcat attcctctaa tagaaacgta atcgcagttt tcattttcaa gctctccttt
    26761 actatctata acaaggacaa cagttttaac ctacagacat tcattaaaat tgggtagcga
    26821 cagcgaaaac aacaattctt aagttttaga cagagtatac agatagtcaa cttaattaaa
    26881 tacgaactaa ataaaaatga caggtaatca tttttgttta ccagcattat aacattttgc
    26941 atacatggat gacaaaagaa ccaattccca cttgcttgag tacaatatta aaagaaactg
    27001 gaaggcaaaa agtaattata gaaacgaaaa ataaatcata tcgggcactc agacatgaaa
    27061 acaaccctag agatatataa ctaggtaacc agtttaatga aagaaaaaat catccaagaa
    27121 gtcgattctt ggatttttta gtggatttgt tcgccaaata cgtttagtat gcgtatatga
    27181 gaggttgtaa gcaaagcgtt gtggtctgag cacttaagga ggaatctgtg aaaatagctt
    27241 ttcatatttt tgcagatttt gacttaatgc cgaaggtcag ctttgcgatt accgtttatc
    27301 gagaggctgt aaaagcaccg tgtagctccg atgacaataa gcaagcattt ttagagctag
    27361 gtttccttga ttctaaatat gcggcttatt tcaatgagtt ggtgcttgaa cgcgatttta
    27421 agaaggtcga aaacaggcac gacttttcga cgtaaggaga aagaatttca atgatagaaa
    27481 caataaatcc cgcagaaaac aaggtttctg cgtacattca tatcccattt tgcgagcata
    27541 tctgctatta ctgtgatttt aataaagttt ttttagaagg gcagccagta gatgagtatg
    27601 tggaaatgct gctgaatgaa atgaaaataa tgctagaaag tcatccgaca gcgaatttag
    27661 aaacacttta cgtagggggc gggacaccaa cttctttatc tgctaaacaa cttgatcgac
    27721 tgttatcagg tgctagagag atcctgcctt ttcaaaatgg taatgagttt actgtagaag
    27781 cgaatccagg tgatttgacc cgtgaaaaac ttcaagtaat gaaaaactat ggcgtcaatc
    27841 gtttatccat gggagtgcag acatttgata atcgcttatt gaaaaaaatt ggacgtaaac
    27901 acaccgcaga agatgtgtat caaactatgc aatttttaga agcagaaaat tttaccaatg
    27961 taagcatcga tttgatttat gcattaccgg gacaaacact agaaggatat ctggatactt
    28021 tggaaaaagc tttggctttg gatctgcctc actattcttt gtactcattg atcctcgaaa
    28081 acaaaacaat gttcatgaac tgggtacgac aaggaagact cgaactgcct gatcaggaaa
    28141 cggaaaccag aatgtttgaa gagacgattg aagcaatgga aaagcatgga cgtcaccaat
    28201 atgaaataag taactttggg ctctccggac atgaatctca acataacctc atgtactgga
    28261 acaatgacca ctattttggg tttggagctg gtgctagcgg ttatcttgga gatattcgtt
    28321 accgcaacaa aggaccaatc caacactatt tacgcccatt acgagcaaat gaacgcccgg
    28381 tattggaaga agaaaaattg acacgaaaaa atcaaataga agaagaaatg ttcttaggac
    28441 taagaaaaaa aacagggatt actaaaaaac atttctatga tcgctatcaa aaaacaattg
    28501 aatcggtgta cggtgatgtg ctaagggaat tagagcaaga agggttactt gttaatgaag
    28561 aagaccgtgt ttatttgaca ccaagaggga cctttttggg gaacaatgtg tttgagcgct
    28621 ttttgtttga aaaatagttt aaagaatatt tgttttttat ctgaatttaa ataaaaaaag
    28681 ttcaaaaata caataaagga ctattgaaac gttgatataa ctggatttac atcaattatt
    28741 tagaattagc actcttgtgt ttagagtgct aaaaaatgga gtctttctct tgacaggaga
    28801 agggcttctt gctatattta taagtggatt agcacttaga atatatgagt gctaatgaga
    28861 ggtgaggaag atgttaacaa agcgacaaaa cgacatttta cgtctgatca tccaacacta
    28921 tacgagtagt ggcgtgcccg ttggttccaa aacattgatg tctgagggag tcaaggccag
    28981 ctcagcaacg attcgcaatg atatgaaagc attagaagat gaaggattgc tgttgaagac
    29041 tcactcttct tctgggcgga ttccatctgc tttaggctat cgttattacg tggatcattt
    29101 gttaaaacct gctcgcgtag cagaagatga tttgctgctg attcgtcagt ctctaggcag
    29161 agaatttcat gaaatcaatg agattattaa gcaatctgct gagatattat ctgagctgac
    29221 aagctatacg actttttcat tgggacctga aataaaagac cgccgactga ctggctttcg
    29281 tatcgtaccc ttgaataatc gacagattat tgctatcgtc gtaacagata tggggaatgt
    29341 agaaagccaa gtgttcactc ttcccgaata tatgggaagt caggaactag aaaaaatggt
    29401 acagattgta aacgaccgtc tagtcggaga tcctctgatg acggtgtacc ataaacttcg
    29461 tacggagatt ccgatgattc ttcataaata tttccaaacg acagaaggga tgtctcagtt
    29521 atttgacacc gttcttggtc atgtttttga agataaggtt tatgtcagtg gacaaatgaa
    29581 tctcttggat tatgagcccc gtcaagatct tgatcagttc aaatcgatgt tttcttttat
    29641 gaaagactca gacggactta cacaaatgat cactccgatg gatagcaata ttcatatcaa
    29701 gattggtgct gaattaggca atgatttatt gcagaatatg agtatgattc aagcaagtta
    29761 cgagatagat ggtcatggaa gaggtacaat agccttgtta gggcctacta gtatgcctta
    29821 ttctcggatg ttcggtctag tcgatgttta tcgaagagaa ctagcagcta aacttgctga
    29881 ttactaccgt tcgcttgact tgtcaaactc gtaatgtgaa taacgaagct atttgcagct
    29941 gatcgttctc tctttcttag taagagcaat caaacagctg actgaataat gaaaggaaga
    30001 ctgtatcgtg aaagaaaatc aagaagaact agataaagaa atgacagatg ctcagccaga
    30061 accggaaatc gatgtcgaag ctgcagaaga ttcgggtatt tcagaagctg aagcggaaga
    30121 attcgaaaca gcgaaattaa aagctgagtt agaagaaatg gaagataaat acttgcgggc
    30181 tcgtgctgaa atcgcaaata tggcaaatcg aggaaaaaat gagcgtgagc aattacaaaa
    30241 ataccgttca caagatttag ccaaaaaatt acttccatca attgacaacc tggaacgtgc
    30301 attagccaca gaagtgtcag atgatcaagg ggcaggtttg aaaaaaggcg tggaaatggt
    30361 cttggaaagt ttgcgtaacg cactggaaga agaaggaatt gaaaaaatcc ctgctaaagg
    30421 agaagccttt gatcctaatc ttcatcaagc agtacaaact gttccagcga ctgaagatac
    30481 accagccgat acgattgtag aagtactaca agaaggctac aaattacatg atcgcgtgtt
    30541 aagaccaacc atggtcattg ttgcacaata attgtgcgga tataaaactt acactttcaa
    30601 ttaaagaagg agacgttaat tatgagtaaa attatcggta ttgacttagg tacgactaac
    30661 tctgcagtag cagtattgga aggaaacgaa gcaaaaatca tcgctaaccc agaaggaaac
    30721 cgtacaacac cttctgttgt atcattcaaa aatggagaaa tccaagtagg ggaagttgca
    30781 aaacgtcaag ctgtgacaaa cccaaatacg atttcatcaa tcaaacgcca tatcggtgaa
    30841 gcaggttaca aagtagatgt agaaggtaaa tcatatacac cacaagaaat ctcagcaatg
    30901 atccttcaat atatcaaagg attcgcagaa gactatttag gtgaaaaagt cgaaaaagca
    30961 gttatcactg tgccagctta ctttaatgat gcacaacgtc aagcaacaaa agatgctggt
    31021 aagatcgctg gtctagaagt agaacgtatc gtcaatgaac caactgcagc tgctcttgcc
    31081 tatggtttag ataaaacaga tcgcgacgaa aaaatcttag tattcgacct tggtggcggt
    31141 acttttgacg tatctatcct tgaactagga gacggtgtct ttgatgtatt gtcaacagct
    31201 ggcgacaacc atctaggtgg ggacgacttt gataacaaaa tcatcgacta catggtagca
    31261 gaattcaaaa aagaaaacag cattgacttg tctcaagaca aaatggcttt acaacgcttg
    31321 aaagacgctg ctgaaaaagc gaaaaaagat ttatctggcg tatcaagcac acaaatcagc
    31381 ttgccattta tcacagcagg agaatctgga ccattgcatt tggaaatgac attgacacgt
    31441 gcaaaatttg atgaattaac agctgactta gtagaacgta caaaagtacc agtacgtcaa
    31501 gcgctaaaag atgcaggttt atcacaatca gaaatcgacg aagttatttt agtcggtgga
    31561 tctactcgta tccctgcagt tgtagaagca gtacgtaaag aaacaggaaa agaaccaaac
    31621 aaatcagtta accctgatga agtagttgct atgggtgctg ctatccaagg tggcgtgatc
    31681 actggtgatg tgaaagatgt tgttttactt gacgttacac cattgtcatt aggtatcgaa
    31741 acaatgggtg gagtgttcac taaattgatt gaccgcaata caacgattcc aacaagtaaa
    31801 tcacaagtat tctcaactgc ggcagataat caaccagcag tagacatcca tgtattacaa
    31861 ggtgaacgcc caatggctgc tgacaacaaa actctaggaa gattccaatt gacagatatt
    31921 ccagcagctc ctcgtggtat tccacaaatc gaagtaacat tcgatattga taaaaacggg
    31981 attgtaaatg tatctgcgaa agatctaggt acacaaaaag aacaaaaaat cacgatcaaa
    32041 tcttcttcag gtctaacaga cgaagaaatc gaacgtatgg taaaagatgc agaagcaaat
    32101 gcagaagcag ataaagctcg taaagaggaa gttgacctac gtaatgatgt agatgcattg
    32161 ctattctctg tggacaaaac attgaaagaa ttagaaggca aagtggacga agaagaagtg
    32221 aaaaaagcag aaacagctcg cgatgaattg aaagccgctg ttgaagcaaa caacatcgaa
    32281 gaaatgaaaa ctaaacgcga tgcattgaac gaaatcgttc aaaacttgac tgttaaacta
    32341 tacgaacaag cggcacaaca acaagctcaa gaaaacccag aagcagctca aggcggagca
    32401 gatgatgtcg ttgatgctga ttttgaagaa gtcgatggcg acgataaata atcagttcac
    32461 tgaatttcga gagaaggcca aagttgctac tttggctttt tcttttgaaa aatagtttga
    32521 agtatcggtt tttgtttcaa acaaaatatg atatgataac aagcgacgtg aaaatgatga
    32581 gaaccaatag gaggaaagcc tatggcaaca aaacgtgatt attatgaagt cttagggctc
    32641 tcaaaaggag catcagaaga tgaaataaag aaagcttatc gtaagctttc gaaaaaatac
    32701 catcctgaca tcaataaaga acctgatgcg gaagaaaaat tcaaagaagt atcagaagca
    32761 tatgagattt taagtgaccc gcaaaaacgc gctgcatatg accaatacgg gcatgcaggc
    32821 acagatccaa attacggtgc tggtggagct ggtggcggat ttggtggatt cggcggcttc
    32881 tctggtggcg gcttcggtgg ttttgaagat atcttcgact ctttctttgg aggaggcggt
    32941 cggtcagtcg atccaaatgc gcctcgtcaa ggtgctgatt tgcaatatac ggtcgacttg
    33001 tcttttgaag aagcgatttt tggtgtagaa aaagaaatca agtataatcg ggaagaaatc
    33061 tgtcatactt gtggtgggaa cggtgcaaaa ccaggcacac aaccaactac ttgtcataaa
    33121 tgtcatggtg caggtacgat taatgtagaa cgccaaacac cacttggacg agtgatgagc
    33181 cgtcaaactt gtgatgtttg tcatgggaca gggaaagaaa tcaaagaacc ttgtccaacc
    33241 tgtcatggct caggacatga gaagaaagca cattcagtta aagtgaatgt accagctggt
    33301 gtagaagaag gacaacaaat gcgtttagct ggtcaaggag aagctggaga aaacggcgga
    33361 ccatttggtg atttgtatgt ggtcttccgt gtagaagaaa gtgatatctt tgatcgtgaa
    33421 ggttctgaaa tttactatga attgccttta agttttgtac aagcagcttt gggggatgaa
    33481 gtccaagtac caactgtcca tggagatgtg aaattgaaga tccctgctgg aacacagaca
    33541 ggtacgaact tccgtttacg tggaaaaggc gctccgaagt tacgtggtgg tgcaacaggc
    33601 gaccaacatg tcaaagtaaa actgatcaca ccgaaaaatc taaatgaaca acaaagagaa
    33661 gcattacgtg cattcgctga agctggcggc atcaaagttg aagaacagca agaagacggt
    33721 ttcttcgata agtttaaaga tgcttttggc gggaaaaagc ggaagtagtt tttctagtaa
    33781 aaacgctaag tcattaataa ttaaaaacca gacgaacaat catatgttcg cctggttttt
    33841 ttgtagttgt catgtttgta ccaacaaaaa agccagcttt agaatatgat aatcgtcata
    33901 gtttctaggg gtataaccag tgagagaatg gaacttgttc aatcgatatt gcaaggtgtt
    33961 tttatgaata aataattctt ctgagcaatt tttgatgctt ccgttatttt tactgaagca
    34021 tgccaaagtc ttcttgaaca aatgtacttc ttcttctgtc aggcttttca aaatacgttt
    34081 tacatataat gttcgttgat ctgacgggat tgctggtaaa acgatcccga ggtctaaatc
    34141 ttcaaaaaag cggatctcgt tcacagattg atggaaagct atccattcgc aaacttgttt
    34201 ggcatgaagg aaagattgcg gtgtctcttg ctctgtataa caaagcgacc caattccaaa
    34261 gtttagctgt aactgataca ttttattaaa ctttgtctgg attttatcaa gcaatgatag
    34321 caatgaggct ctggaaatcc gattgactag gataatgatt tgttgtctat gtacagtgag
    34381 caaagcctgt ccgtaataag agaagaccct ttcgagcaac tccaataaag gattgatccg
    34441 ttcttgacac aattgctgat tgtcagaaag ttgtcccaca actacataat atggagaaga
    34501 aaaatcaagc tcaattaagt gagggtggta tcgactatgt acttgatatc atcaatgaac
    34561 cgatggtact ttcttatgcg atggaacagg tagagcgatt gcttgtttgt gcaggaaaac
    34621 aagcgatgcg gctgatacga ttaggtgaga aaataagaag ataaaaatac acggtccctt
    34681 catggcgacc gtgcattttt ggtttttatt aaatatttaa tactttattc aagaaatctt
    34741 ttgtccgttc attttgcgga ttttcaaaga tttgttgagg cgttccatct tctaaaaagt
    34801 ttccgtcgtc aatgaacatg acgcgattgg ccacttcttt agcgaacccc atttcgtgcg
    34861 taacgataac catcgtcata ccttgttctg ctaacttttt catgacgttc aagacatcac
    34921 cgaccatttc aggatcgagt gcagaagtag gctcatcaaa aagcatgatg tcagggttca
    34981 tcgctagagc acgagcaata gccacacgct gtttttgtcc accagaaagc atttcaggat
    35041 acatatcttt tttatcttct agaccaactg tctgcagtaa tttgattgct tttttctctg
    35101 cttcttctct agattctttc ttcaggtcca tcggtgctaa aatgatgttt tccaaaatag
    35161 aaagatgagg gaataggtta aagtgttgga ataccattcc gatatgttgt ctcaccaagt
    35221 tgatatctgt gcttttatcc atcaagtggg cgccgtcaat aataatttca ccgtcagaag
    35281 gttcttctaa acgattcaag caacgcaaga atgtactttt tcctgaacct gaagggccaa
    35341 tgatacagac gacatctcct tcgttgatgg atacattgat gtcgtttaat actgtgttat
    35401 cgccgtattt tttgactaaa tgctcaacta agattttttc tgccatctta cttcaccttc
    35461 ttttctaaaa cttttgctaa ttttgtcaat gctgtgatca aaatcaagta catgatcgca
    35521 atgatcaagt aaacatacgt actttgtgtt gtacgtgcaa caatgatctt accagtttgc
    35581 agcaattcga ctacaccgat tgcggaaatg atcgttgtgt cttttaatga gataacgaac
    35641 tgattgacga atgatgggat catgatgcgt atcgcttgcg gcaaaatgat tttttgcatt
    35701 gtccgattat aagaaagacc taaacttctg gaagcttcca tttgtccgac aggaacggcg
    35761 ttgatcccgc ctcgaacaat ctctgcgata taagcacttg cgttaagagt caaggtgata
    35821 acaccagcaa ggaagtcagg aatcgtaaat ccaacgatac caggcaaccc aaagaaaata
    35881 aagaacgcaa gaaccatcat tggaattcct cggatgatat cgacataaat actagcaatt
    35941 gtgcgaagag cgcgtattgg tgctacactg aataatccaa aaatcacacc gattactata
    36001 gccaaagcaa aggagatcaa agttaggaga attgttctcc aaagtccttt caacaatgct
    36061 tgatagttgt tttggataag tccaagaaat gttgattcat cagcagtgga agactcactg
    36121 gaagtttctg aaccatccgc tacatattct ttaataatct tatcgtattc gcctgttctt
    36181 tttaattctt tcaatccttc attgaacata ttaagaagct caggattttg tcctttttta
    36241 acggcaaatg catattttcc gccagattct tttttgatcg gtgtttccaa ttcttgtcct
    36301 tgtttgatcg cataaccgat taccggataa tcatccatca ttgcatcgat tgcgccaact
    36361 tttaacccgt cgtacaattg atcagcagca tcaaaatatt tgatagaata gccatattca
    36421 gattcatggt cgcttaaaaa ttcagcactt tcggtaccaa cttttgctcc gactgttttt
    36481 cctttcaaat cttcataaga cttgatcgaa gtatctcctt ttttgacagc taattggatc
    36541 ccgctatcaa agtaagaatc agaaaaatca tagcttttct ttcgttcatc tgttacagtc
    36601 attccggcaa tcattccgtc tgcttgtcct gattccaaag cttgcaaagc agcagagaaa
    36661 ccacggtagt ccattcggat gttaaatcct tgcagtttgg cgatgttttc catcaatttt
    36721 acatcgatgc cttcgtactc gccctttgcg ttttgaaatt caaatggagc aaaagcagag
    36781 tcactggcaa ttacgtatac gtcttttttg ggctctattt tttgcattgt ctcgcttgtg
    36841 cttgactctg ccgttgtagt gttgtcagct tgtgcaacat gaccaaacgc aaatgtcaca
    36901 agtcctgtca ttaagaaaac aaatgagtga aataaatgct ttcttttcat aatcaacctc
    36961 ctaaatttcc aatattttat tattataggc gaaaagtgct gaatcagaca agactgtaag
    37021 ctcgaaaaaa tgaaaaaaat gcgagtttct aacattttat gttataaaac ataacatgta
    37081 taataagata aaaaagtggc gaaagtatgt tcggtgtggt ataatttttt agtaaggaat
    37141 gcctttgctt tccttttctt tttattggtc gtaaaatgaa aaggaatgaa tgaaaaggag
    37201 gacgaagaat gattgagaga gatacctccc gacactttga gcttgtctcg aaataccaac
    37261 cggctggtga tcagcctgaa gcgattaacc aattagtgga tggcgtagtc ggcgggaaaa
    37321 aagcacaaat cttattaggt gcgactggaa cagggaaaac atatacgatt tccaatctta
    37381 ttgagaaagt gaataaacca acactgatta ttgcccataa caaaacattg gctggtcagc
    37441 tgtacgggga attcaaggaa tttttcccta acaatgctgt tgaatatttt gtttcttatt
    37501 atgattatta tcagcctgaa gcttatgtgc cttcaagcga tacgtatatc gaaaaagatt
    37561 caagtgtaaa tgatgagatc gacaaattac gacattcggc tactagttca ttattagaaa
    37621 gaaatgatgt gatcgtcatt gcatccgttt catgtatttt tggattaggc tcgccttttg
    37681 agtaccaaaa acaagtagtt tcgattcgac agggagcaga actagatcgc aatcagttga
    37741 ttcgtgactt agtcagtatc caattcgaaa gaaatgatat agattttcag cgcggtcgtt
    37801 ttcgagtgcg cggtgatgtc gtggagattt ttccggcttc aagagatgaa cgtgccttac
    37861 gtgtggagtt ttttggagat gaggtcgaac gtatccgaga agtcaatgca ctgacaggtg
    37921 aagttttagg agaaacggaa cacgtagcaa tcttcccggc gactcacttt gtgacgaatg
    37981 atgaacatat ggagcatgcc gtagcgaata tcaaagcgga attagaacag cgtttaactg
    38041 tcttgcgaaa tgaaaacaaa cttctggaag ctcaacgttt agaacagcga accaattacg
    38101 atattgaaat gatgttagaa atgggctaca cgtcgggaat tgaaaattat tctcggcata
    38161 tggacggacg taaagaaggt gaaccgcctt atacgctatt ggatttcttt cctgaagatt
    38221 ttttgatcgt ggcggacgaa tcgcacgtaa ccatgcctca aatcagagga atgtataatg
    38281 gtgaccgagc gagaaaacag atgctagtag attacggttt ccgtttgcca tctgctttag
    38341 acaatcggcc acttcggtta gaagagtttg aaaaacatgt caatcagatc atttatgtct
    38401 ctgcgacgcc aggaccatat gaacatgaac aaacagatac cgttatccaa caaatcatcc
    38461 gtccgacagg gctgcttgac ccagtgatcg aagttcgtcc aatcatggga caaatcgatg
    38521 atctagtagg cgaaataaat gaacgagtcg aaaaagacca gcgggtgttt gtaacgacat
    38581 tgacgaaaaa aatggcagaa gacctcacag attatttcaa agaattaggg atcaaagtca
    38641 aatatttgca tagtgatatc aaaacgctgg aacgaacaga aatcattcga gatcttcgat
    38701 taggtgaatt tgatgtgtta gttggaatca atttgcttcg tgaaggatta gacgtacctg
    38761 aagtttccct tgttgctatc ttggatgcgg ataaagaagg ctttttacgt agtgaacgtt
    38821 ccttagtcca aacgattgga cgagctgctc ggaatgaaga aggtaaagta atcatgtacg
    38881 cagacaaagt aacagattct atgcgtctag cgatggatga aacgtcaaga cgtcgtacga
    38941 ttcagcagaa gtacaatgaa gaacatggga tcgttccaaa aacgatcatt aaagaaatcc
    39001 gagatttgat ttctattacg aaagaatcgg aagatgatac aaaagaagcg gtacaagtct
    39061 cgtacgaaga aatgacgaag gaagaaaaag acacgttatt gatgaaatta gaaaaagaaa
    39121 tgaaagatgc ggcaaaagct ttggattttg aaacagccgc gaatgtgcga gatatgattt
    39181 tagaattaaa agcttcaaag taggaggaca acatggctaa cgataaaatt acgattcatg
    39241 gtgcacgtgc ccataacttg aaaaatatag atgtaacgat tccacgagat aaattcgtgg
    39301 tcgttacagg tttgtccggt tctggaaaaa gttccctagc atttgatacg ctgtacgctg
    39361 aagggcagcg gagatacgta gagagccttt ctgcttatgc tcgtcagttt ttaggacaaa
    39421 tggataagcc ggacgtagat agcatcgacg gattgagtcc ggcgatttct attgatcaga
    39481 aaacaacaag taaaaatcct cggtcgacag taggtactgt gacggaaatc aatgactatt
    39541 tacgcttgtt gtttgcacgt gtaggacatc cgatttgtcc caacgatcat atcgagatta
    39601 ccagtcaatc agtagatcaa atggcagatc aagtaatggc tttgcctgag cgcacaaaga
    39661 tccaagtgtt ggcaccgatc gtggtaaaga aaaaaggaca acataaaaaa atattcgaac
    39721 gaatccaaaa agagggatac gtccgcgttc gagtcgatgg tgagatctat gatttaagtg
    39781 aagtgcctga gctggagaaa aacaaaaaac atgatatcgc gatcgtcatc gaccggatca
    39841 ttgtgaaaga agggattcgt tctcgcctat ttgattcttt agaggcggcc ctgcgattag
    39901 cagacggcta tgctctggtt gacgtgatcg gtgaagaaga aatgttgttt agcgaacact
    39961 atgcctgtcc ttattgcgga tttactgttg gcgaactaga accaagactg ttctcattca
    40021 atgcgccttt tggagcttgt ccagactgtg atggtttagg tgtcaaatta gaagtcgatc
    40081 aagatttagt gattccagat agaacgaaga cacttagtca aggtgctatc gttccatgga
    40141 atccaatcag ctctcagtat tatcctcaaa tgttagcgca ggcttgtgaa agtttcggta
    40201 tcgatttaga tacaccattc gaagaattac cggaagagca tcaggagatc atcttgaatg
    40261 ggacagatga tcttttccat ttccattatg aaaatgattt tggtggcgtt cgcgatgtag
    40321 aaacgacttt cgaaggggta ttgaagaata tcgagcgtcg ttatcatgat acgaacagtg
    40381 atttcacgcg tgagcaaatg cgtctttata tgacagaatt aacttgtcag acatgtaaag
    40441 gttatcgatt gaatccccaa gctctatctg ttcaaatcaa tggaacgaat atcgggcaaa
    40501 caaatgagtt gtctattcaa aaagcagtag atttcttctc aaacttaacg ttatctgaac
    40561 aagagaaagt aattgccaaa ccgattttaa aagaagtaaa tgacagactt tcattcttgg
    40621 aaaatgtggg actagactat ctaactttga gtcgcgcttc cggaacatta tctgggggag
    40681 aagcgcagcg aatcagattg gctacacaaa ttggatcgaa tctttctgga gttttgtata
    40741 ttttagatga accatcgatc ggattgcatc aacgcgacaa cgatcgactg attggctcat
    40801 taaagaaaat gcgtgatctt ggcaacaccc tcgttgtagt cgaacatgat gaagatacga
    40861 tgtgggcagc agattatttg attgacgtcg gacctggggc cggagaacaa ggcggacaaa
    40921 tcgtggctgc aggcactcct gaagaagtag ctaatgatga aaattctttg acagggaaat
    40981 atctttcagg gaaaaaatca atccctgtac caaaagaacg ccgtaaaggt aatgggaaag
    41041 caatcaagat cactggtgct tcagaaaaca atctgaaaaa tattagtgtt gaatttccat
    41101 taggagaatt tgtagcagtc acaggtgtgt ctggttctgg aaagagtaca ttggtcaata
    41161 gtatcttaaa gaaatcgtta gcgcaaaaat taaataagaa ttctgctaag ccaggtaaat
    41221 tcaagacaat ttccggctac gaaagtatcg aaaagatcat cgatatcgat caaagcccaa
    41281 tcggccggac gccgagaagt aatccagcga cttatacaag tgtatttgat gatatccgtg
    41341 ggttatttgc tcaaacgaac gaggcaaaaa tgcggggtta taagaaaggg cgttttagtt
    41401 tcaacgtaaa aggcggtcgt tgtgaagctt gtcgcgggga tggaattatt aagatcgaaa
    41461 tgcacttttt gcctgatgtc tatgttcctt gtgaagtatg tcatggtaaa cgatataatt
    41521 ctgaaacatt agaagtgcat tacaaaggaa aaagcattgc tgatattttg gaaatgacag
    41581 tagaggatgc tgtagaattc ttcaagcaca ttccaaagat tcatcgcaaa ctgcaaacga
    41641 ttgttgatgt tggcttaggt tatgtgacga tggggcaacc agcaacgaca ttgtccggtg
    41701 gtgaggcaca acggatgaaa cttgccagtg aattgcacaa aatctctaat ggaaagaatt
    41761 tctatatact agatgaacca acgacaggac ttcatagcga tgacatcgcc cgcttgttgc
    41821 atgtattaca aagattagta gatgctggta acacagtttt agtgattgaa cacaatctag
    41881 atgtaatcaa aacagcagat tatatcattg atttaggacc agaaggtgga gaaggtggag
    41941 gaacgatcct tgcgactgga acaccagaag aaatcattaa cgtaaaagaa agttatacag
    42001 gtcactattt gaaaaaaata atggtataaa gaggatagat agctgagata gttaaaaaaa
    42061 cataaaaaaa tgaatttttg cttgttttat taatatcagt gttctagaat cattacatgg
    42121 tagttctttc tacactgatt ggtatagata aaaacttcct ataagttcaa tccgttcaaa
    42181 aaagaattga gcaatcggag gactttcctt tgattgcttt tttctttatg gtatgatatt
    42241 ccttgtgtag ttggaaaaga aaaacaagag attatgagta agtgagagat aatgatgaac
    42301 agtattgaaa atgaagaatt tatccctata ttattaggaa gcgacatgaa cgtttatggt
    42361 atggcacgtt cttttaatga agcatacggc aaaatctgcc aagcatatgc ttcggatcag
    42421 ttagccccta cccgctacag taaaattgtc aatgtagaag ttattccagg atttgataaa
    42481 gaccctgtat ttatcgagac gatgcttcgt ttggcaaaag aaagatattc tgataagagt
    42541 aaaaaatatc ttttgattgc ttgtggtgat ggatatgcag aattgatctc ccaacataaa
    42601 caagagctgt cagaatattt tatctgtcca tatattgatt attctttatt tgaaaggctg
    42661 atcaataaag taagctttta tgaagtttgt gaagaatatg atcttccgta tcctaaaaca
    42721 ttgatcgtca gagaagaaat gttagtaaat ggacatttgg aacaggaatt gccttttgaa
    42781 ttcccagttg ctttgaaacc tgcaaacagt gtggagtatc tttctgtcca atttgaagga
    42841 agaaaaaaag cttttatttt agagactaga gaagagttcg atttaatttt aggccggata
    42901 tacgaagccg gttataaaag tgaaatgatt gtacaagatt ttatcccggg tgatgatagt
    42961 aatatgcgtg tgctaaatgc gtatgtagat gaagatcatc aagttcggat gatgtgcctt
    43021 ggtcatccac tattagaaga tcctacacca gcatcgattg gtaattacgt tgtgatcatg
    43081 ccagattata acgaaaaaat ctatcaaacc attaaggcat ttttggaaaa aatcgaatat
    43141 acaggctttg ccaatttcga tatgaagtat gatcctagag atggagaata taaattattt
    43201 gaaatcaatt tgagacaagg tcgtagcagt ttctttgtga cattgaacgg cttgaatctg
    43261 gctcgttttg tcacagaaga tcgagtgttc aacaaaccct ttgttgaaac gacctatgga
    43321 acgaatcaat cagataaagc aagattatgg atgggtgtgc ccaaaaaaat ctttttagaa
    43381 tatgctcggg aaaatgaaga caaaaaacta gctgagcaaa tgatcaaaga aaatcgttac
    43441 ggtactactg tattttatga aaaagaccga tcaatcaagc gctggttgtt gatgaaatat
    43501 atgttccata actatattcc aagattcaaa aagtattttc atgtgaaaga aggctagttc
    43561 atggagaatt ttttcagtat tttaggcgga atgggcacga tggcgacaga aagttttgtt
    43621 cgcttgatca accaccgaac gaaagctaca aaagatcaag aatatttgaa ttatgtctta
    43681 ttcaatcatg caacagttcc tgatcgtaca gcttatattt tagatcgatc agaagaaaat
    43741 ccaatgccgt ttctgctgga tgatattgag aaacaaaatt tattgcgacc aaacttcatt
    43801 gtgttgactt gtaacaccgc acactacttt tttgaagagt tacaagcggc aacggatatt
    43861 cctatcttgc atatgccaag agaagcagca aatgaacttg tccgtcagca tacgacaggg
    43921 agagtagcga ttctaggaac agaaggaagt atgaaagctg gaatctatga acgcgaagtc
    43981 aaaaatcttg gtttcgaaac agtgattcct gatactgcgc ttcaagaaaa aatcaattac
    44041 ctgatctatc atgagatcaa ggaatcggat tatttgaatc aagagctcta ttacgaaatc
    44101 ttagaggaag cggtagagcg cctaaactgt gaaaaagtca tcctaggttg cacagaatta
    44161 tctttgatgc atgaatttgc tgaagataat cattacccag tgatcgatgc tcaatctatt
    44221 ttagcggatc gaacgatcga acgtgcatta gctgaaagaa gcgaggcatt ggatacagca
    44281 tcggaaaagt aaaaaaattg gatttggggc aaatatccta aaagatattt ggtccaaatc
    44341 tttttgtaaa agaaaaagaa aactggtaat gatacgcccg ggatgatata attttattat
    44401 gcacaatgtg agaggggttc gtaaatatga cagataattt gcaattagtc atcataacag
    44461 gtatgagtgg tgcagggaaa acagtagcta tccaaagttt tgaagatatg ggctattttt
    44521 gtatcgataa tatgccgcct cgattgattc caaaattctg ggaattgatc aaagaatcag
    44581 gtaaagtcac aaaaatggct ttaggagtgg atttgcgttc tcgttccttc tttgaagaaa
    44641 tccaaaacat gctgatcgaa atcgaaaata cgaacttcat cgacacttct atcttatttt
    44701 tagatgcttc agacgaagaa ctagtggctc gctacaaaga aacaagacga gcacatccaa
    44761 tggcgatgga cggtttgatc actgaaggga tacgtaaaga acgggcgata ctagacgact
    44821 tgaaagccca agcttccgtg atcattgata cgacaacatt gacaccacga caattacgtg
    44881 aaaaaatcaa tgagtcattc aaatcaaagg gagaccaagg atttcggatc gaatttgtat
    44941 catttggttt taaatacggg ttgccgatcg attcggatat tgtgatggat gtgcgttttt
    45001 tgcccaaccc tcattatatc ccggaactaa gaccattgac cggaaaagat cagccagtct
    45061 atgattatgt gatgtccttt gatgaaacgc aatcattcta ccataaattt ttggatcttc
    45121 tggaaactgt attgcctgga tacattaaag aaggaaaaag cagcttgaca gttgcaatcg
    45181 gctgtacagg cggtcaacac cgttctgtgg cattgactga acgtttggca aatgctttag
    45241 ggaaaaacta taaagtcaat gtgacacatc gggacaagga taaaaggaaa gagacggtga
    45301 accggtcgtg aaaatgaaaa cctaccggat aagaaaacca aagatcgtag tggtcggcgg
    45361 tggcactggt ctgccagtta tcctgaaaag tttacgaaat caaggagcag atatcacagc
    45421 agtagtgact gttgcagatg atggtggaag cagcggtgca atcagagaat cgatcgctat
    45481 ggctcctcct ggtgacttga gaaatgtctt agtggcgtta tcggatatgc cacaattcta
    45541 tgaagatatt ttccaatacc gtttcaaaaa ggaagatcag tttttagcca atcatacgat
    45601 cggtaatctg atcattgccg cagtttccga gatgcgggga agcacttatg aggcgattca
    45661 attattagca aaaatgatgc atgtagatgg acatgtttat ccttcttcag aaactccttt
    45721 gaccttgcat gctgttttta aagacggaac gactgcagta ggagaatcta aaatcgcgat
    45781 caaccgtaaa accattgacc gtgtgtatgt ctgcaacaca aatgataaaa gtgaagccaa
    45841 agcggctcga aaagtcgttt cagcaattat ggacgccgac atggtcgttt tgggacccgg
    45901 aagtttattc accagtatcc ttccaaattt agtgatacca gaaatcggcg aagcaatgct
    45961 caagacaact gctgaaaccg tctatatctg taatatcatg acccaaaaag gggaaacgga
    46021 acatttcaca gatgccgatc atatacgtgt tttacacaag catttgaatc agccttttat
    46081 cgatacagta ttggtcaata cggaaaaagt gcctgaagat tatatggacc cagagatcta
    46141 tgatgagtat ctagtacaag tcgcacacga cttcaacgga ttgagaaatg aaggatgtcg
    46201 agtgatttcc accgactttt tagaattgaa agatggtggt gtcttccacg atggagacaa
    46261 agtagtagaa gaattgttcc gtttagtttt cggttcaaaa tattaagcaa tttattgttg
    46321 gagaaaggag gagaatagga tgtcatttgc tgcagatgtg aagaaagaac taactggttt
    46381 ggaagttcat cgagaacatg cgaaagcgga attagctgct ttgctgcgaa tgaacggctc
    46441 tttaggtatt gtgaaccagc agtttatttt gaacgtccaa acagaaaatg ctgcaattgc
    46501 cagacgtatc tattctcttt tgaaagatca ttatcaggta cgaagtgagc tactcgttcg
    46561 taaaaaaatg aaattaaaaa agaacaatgt ctatatcgtc cggttgaagc aaggcacaaa
    46621 agatgtgttg atggatcttg atatcatgga tggtatgatg ttcaatggac acgtttcgga
    46681 cgagattatg ggaaattcgc aaaaaatgcg ttcttactta cgtggtgctt ttatggcctc
    46741 aggttcagtc aataatccgg aaacgagtcg ttatcatttg gaaatatttt ctatttatga
    46801 acagcataac caagacattt gcgatatgtt aaattattac ggtctacatg ctcgaacgct
    46861 tgaacggcgc aatggctata tttcgtattt aaaaggagca gaaaaaattg ctgatttctt
    46921 aacattgatt ggtgcaacca actcaatgct ccgatttgaa gatgttcgaa tcgtacggga
    46981 tatgagaaat tcggtcaatc gtctagtcaa ttgtgagaca gcaaatctga acaagacgat
    47041 tgatgcggct tctaaacaaa tcgaaaacat cgaattgatc gaagcaaagg tcggtctaca
    47101 tgcccttcct gaaaaacttc aggaaatcgc agaacttcgg cttcaacatc cagaggtcag
    47161 cttgaaagaa ttaggggaaa tgattccctc aggtgcaata tcaaaatcgg gaattaatca
    47221 ccgaattcgc aaaataaatg aatttgcgga aaaactgcgt gaagaagcag tttaatccaa
    47281 agaaaaaact gatacatgaa gcaggagatg cgaatgtatc agtttttttt aaaagctata
    47341 cttgcttttc ttccgtcaaa ctataattcc aactttactg gagtaacata atgttgaaag
    47401 gcttctattg ttttttaata caatcgtata aaaagcgggg agacaactga atgaagattt
    47461 tagttttata cataaacttt tgcttaatat agaagtcctg atatagatag gatcaagtat
    47521 ttatctataa ctggagatta attaaaaact ggaaggaact gctcgatcaa ccttgagaac
    47581 tgtttttctt acttctggtc aagattctcg tggttggtgg ataaggatgg ttgataagtt
    47641 cattggatta gctagtcgaa aacaaccagg tgtaattaat gtagaagttt taacaagggg
    47701 atggagtgat ttattcagtg ataatcataa gttcataaat agtaattggg atgtatggtt
    47761 ggtatacgtt atacctgaac ttgaactatc aagatttggt ttagaatttg agaatcgatg
    47821 tttataaagg gagggcaaaa aatgtgcttt tttacaactt ttactatcat tatgattgca
    47881 gtacttttta tttttctaga tatagcaaag cgaaatacag catttttatt atatcgggtt
    47941 ttacttcgag caggtctcat aacatttata tcgatcgttg gattttttct attcacagtt
    48001 attgtattta tttggagaac tacagcacca ccattgccag agattacata tggagaattt
    48061 ccgtttagtc ttgaatatga aatagatgag gaaagaattt tgattgaaga tacggtgatt
    48121 gcagaattta taggaagttt tagagggaat gtgacaactc aacctagacg aagttggagt
    48181 ataaaacttt taaataatag tggagagttg aaagcatttc attatgaatt cttaataata
    48241 gaaagtgaag atgtaacaat cactttaagt agtggctctg ctggatattt tatgaatgat
    48301 ggtggaagca agccacagct cacagtccgt gatcgtattg caggttttat gcatttttct
    48361 cactatgact tagaagaagc tcgtaaaata cttgcagaat atgggatcac attaatcaat
    48421 tgggaatatg cgccaccgat aagaaatttt ttcaatgatt attattagtc ttataaaaaa
    48481 cagaatgtaa aatttatgtg cgcgagtatt tagttttcca ttttttgttt gatagatata
    48541 tagcattgtt gaagcatggg atagtggtgc caattttatc aatgaggttg gtcaggcagt
    48601 taacataatt ggtgatgcta taaatccatt taattggaat tggaggaatt aggtgtggaa
    48661 acagtgagat tatggatatt catgaaaaat tttcgttcgg ctttgagata tgttattctg
    48721 gaatcaaaat caggttatta tctgattgac acagatcact atactttttg gggaggctac
    48781 ctttttccga tttttaattg gataatcccc cataaggtta tgaaaattaa cttgcctgca
    48841 gaagaaataa atagattaat actcaacaaa gatttacaaa ggaaaagaga gaaagaacat
    48901 tctaatgttg cttggatagg tccagcattt gcagtagtga cttcttcaat tgtcggtcct
    48961 atgctagaga gtatttatag tgatttttct attggaatac gttatttatt tgttacttta
    49021 agtatagctg ttcttttgtg cataaagggt tatgttagca agaagaataa agatatttta
    49081 acactaatcg gtgtaaacaa tttatctagt actaaaatta tactgtttcc tgaatctata
    49141 aaagaaggat caaggttgtt gataattttt ataatgctta tactgttttc tgttttattt
    49201 ggggtgatgt ttata
//
LOCUS       DARBET010000013        48686 bp    DNA     linear   BCT 07-NOV-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4027
            SAMEA114569841-rid20112183.denovo.013, whole genome shotgun
            sequence.
ACCESSION   DARBET010000013 DARBET010000000
VERSION     DARBET010000013.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA114569841
            Sequence Read Archive: ERR12203666
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 48686)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 48686)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001972618.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001972618.1
            Genome Coverage       :: 132x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/07/2023 13:18:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,041
            CDSs (total)                      :: 2,975
            Genes (coding)                    :: 2,908
            CDSs (with protein)               :: 2,908
            Genes (RNA)                       :: 66
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 67
            CDSs (without protein)            :: 67
            Pseudo Genes (ambiguous residues) :: 0 of 67
            Pseudo Genes (frameshifted)       :: 26 of 67
            Pseudo Genes (incomplete)         :: 28 of 67
            Pseudo Genes (internal stop)      :: 20 of 67
            Pseudo Genes (multiple problems)  :: 6 of 67
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..48686
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA114569841-rid20112183.denovo.013"
                     /isolate="VRE4027"
                     /db_xref="taxon:1352"
                     /country="Denmark"
     gene            complement(35..859)
                     /locus_tag="SCJ86_000845"
     CDS             complement(35..859)
                     /locus_tag="SCJ86_000845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326100.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter permease"
                     /protein_id="HEG1168426.1"
                     /translation="MALLSYEFMRRAFLAAIFIAGIAPMLGVFLVIRRQSLMADTLSH
                     VSLAGVALGFFLNLNPTLTTMLVVVLAAVILEYLRTIYRTYSEISIAILMAGGLALAL
                     VLMNLSGGNSATSIQSYLFGSIVTITWEQVIFLGILFVAIGILFFLFKRPMYVLTFDE
                     DTAHVDGLPVHLMSMMFNVITGVAIAVMIPIAGALLISAIMVLPAAIGMRLGKSFNVV
                     IAISVCVGFLGMLSGLTSSFYLDTPPGATITLVFIALFLIVNIIKRILVAFRRKNN"
     gene            complement(832..1512)
                     /locus_tag="SCJ86_000846"
     CDS             complement(832..1512)
                     /locus_tag="SCJ86_000846"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287343.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter ATP-binding protein"
                     /protein_id="HEG1168427.1"
                     /translation="MRYIEISDLTFYYDDEPVLEDVSYHVDAGEFVILTGENGAAKST
                     LVKASLGLLKPSKGMIKIAEKNQEGKKLSIGYIPQQVASFNAGFPSTVIELVRSGRYP
                     RGRWFKRLSEKDHQHVQRALEAVGMWDMRNRRIGELSGGQKQRISLARIFATDPDLFV
                     LDEPTTGMDEESRNEFYRLLRHSAHVHGKSVLMITHDHEDIKQYADRQIRLVRKEDSQ
                     WRCFHMNS"
     gene            complement(1535..2473)
                     /locus_tag="SCJ86_000847"
     CDS             complement(1535..2473)
                     /locus_tag="SCJ86_000847"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ABC transporter substrate-binding protein"
                     /protein_id="HEG1168428.1"
                     /translation="MKKYLLTATVMIGALLFAACGNTNKEADKKEDLTIVTTFYPMYD
                     FTKEIVGDEGNVKLLIPAGTEPHDFEPSAKERAEISDADVFVYNSSDMEFFVDSLKDS
                     VDSKQTLMIEAAKGIDRLESQEADEHEESEEGHGHSHEYDPHVWLDPVLAIKEVRTIA
                     EELGEKYPDKKETFTKNADAYIKKLEALDQKYSDELKDATNRTFVTQHAAFAYLANQY
                     DLKQVAISGVSPDQEPTPSRLAELKEFVKKNNIKVIYFEENASSKVAETLSNETGVKL
                     EVLNPLESLTNEQIKAGENYISVMEKNLKALKESIN"
     gene            complement(2718..3581)
                     /locus_tag="SCJ86_000848"
     CDS             complement(2718..3581)
                     /locus_tag="SCJ86_000848"
                     /EC_number="2.7.1.148"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373455.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-(cytidine
                     5'-diphospho)-2-C-methyl-D-erythritol kinase"
                     /protein_id="HEG1168429.1"
                     /translation="MGERMELIEKAPAKINLGLDVLYRRQDGYHELEMVMSSVDLADH
                     LFFEEITEDKIIVETNKAFLPVDERNNVYQAALLLKNKYNIKTGVKIFVKKNIPVAAG
                     LGGGSTDCAAALRGINQLWNLGCSLEELAEIGLEVGTDVPYCVYGSTAFVGGKGEEIQ
                     VLPSMPQCWVVLVKPRMSVSTRTVFREVDTQAIQHQDIHGLAEAIREQDYQQMIKKMG
                     NSLEDITGKRHPVIHQIKERMIKYGADAALMSGSGPTVFALCQHYSRAQRVYNGLKGF
                     CEEVYLVRTLQ"
     gene            complement(3723..6296)
                     /gene="mprF"
                     /locus_tag="SCJ86_000849"
     CDS             complement(3723..6296)
                     /gene="mprF"
                     /locus_tag="SCJ86_000849"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional lysylphosphatidylglycerol
                     flippase/synthetase MprF"
                     /protein_id="HEG1168430.1"
                     /translation="MKNKRYLMLQWIKEHSLILKLIFFGSVLVFVANQVANIANGMSW
                     QDIWQRMGQQSRMTILLMILAGLLGVTPMLLYDWVTIRVLEKQGKPKMERKEFLLAAW
                     VTNTINNLAGFGGVVGASLRASFYGKQTNRKMVLATVSKVALFMLTGLSVWSFLTFID
                     VFFIQSESIFRSYWIWLFGGSFLAPVIFFFAYLKRKTLFFEFYPKGLLGLLLASFGQW
                     TGALAVFLIIGKLMRIDISMLSIYPMFMIATLIGMLTMVPGGMGTFDVLMILGMSQLS
                     VRQDVAVVWLLYYRLFYYVLPFMTGLLLFITHTGVKINRFLDNIPRLFFQKVAHIVLV
                     SAVYFAGIMMVLLSTITNLSAISRLFQVLLPFSFNFLDQTVNLLVGFLLLGLARALSV
                     KVKKAFLPTIALLSFGILNTITRTLSWQLILVYVLILLAVWLSRKEFYRTKFVYSWGA
                     IIFDASLFGFLFIVYAIAGYHSGRFLVGRITGNHFILFPSDDVWFSGLIGLGISLLAL
                     VALYQYLAEDTTKLGSEYEKDRFDRLLETYGGTATSHQLRLSGYSYFYYQEDDQDQVV
                     FGYQIKGNKCFVLGDPVGNPKMMRAATNQFMKQADCLGYQLAFYKISESYVVMLHELG
                     FHFTKVGEAGIVDLATEDLSRLSQQLEYQRLMKEGYHFTWYDQLPEELHQAVNEVSQK
                     WLQGAREKYFSVGRFDKSYVLSSGVGIVRKDQKVVGFITAQPMTKKQAGYDLLRVLPK
                     ESEELADYLLANLFIVYEKMGYSEANLGLAPLANVGETDFSFFQEKVMHIVYNYGNFF
                     YSFQSAYEKKLKYVDHWEGRYFAYRKGSNFGFATMQLLSLIGKEKGNVPSLAEEVLTE
                     L"
     gene            6437..7243
                     /gene="proC"
                     /locus_tag="SCJ86_000850"
     CDS             6437..7243
                     /gene="proC"
                     /locus_tag="SCJ86_000850"
                     /EC_number="1.5.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyrroline-5-carboxylate reductase"
                     /protein_id="HEG1168431.1"
                     /translation="MKVGFIGTGNMAQAIIDGLLKKGTVSKEEVFVSSGHFENAQKFA
                     AETGVHACKSNQEVASQSDIVLLAVKPKIIEKVLEELREYSQKVLFISIASGKSLIDL
                     ETALEDPQASIVRVMPNVNVSVGAGVSAICKNEAATEEQLQQATMIFEAIGSVYPLAE
                     SDFSTFIGLAGSSPAFVYMLIDAMGRTGVLNGLSKQTATEIAAKAIMGSCQKFLSSDK
                     NPWELVDQVSSPGGTTVAGVVALEEAGFIPAIIKGINTTIEKDLEMLNKK"
     gene            complement(7322..8878)
                     /gene="rny"
                     /locus_tag="SCJ86_000851"
     CDS             complement(7322..8878)
                     /gene="rny"
                     /locus_tag="SCJ86_000851"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875117.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease Y"
                     /protein_id="HEG1168432.1"
                     /translation="MVFNILLAIIGLIVGLGLGFMVAKSRHEKEIAGAQSSAAGIIDS
                     AKKEAETLKKEALLEAKEENQKYRSEVESELRESKLELKSQENRLIQREQTLNRKDDS
                     LEKRENSLESKEEALSSKQQLIEEREKDVEKMIEDQQKELEKIAGLSKEDARDVIMKS
                     TEEELNHELTLMVKESEQRAKEEADRKAKNLLSLAIQRCAADQVSETTVSVVTLPNDE
                     MKGRIIGREGRNIRTLETLTGIDLIIDDTPEAVVLSGFDPIRREIARMTLEKLIQDGR
                     IHPARIEEMVEKSRKEMDERIREYGEEAAFEVGAHTLHPDLIKIIGRLRFRTSYGQNV
                     LKHSVEVAKLAGILAAELGEDIQLAKRAGLLHDIGKALDHEIEGSHVEIGAELAAKYK
                     ENPVVINAIASHHGDVEATSVISVLVAAADALSAARPGARSESLENYIRRLRSLENIS
                     NSFAGVESSYAVQAGREVRVMVKPEEISDLDAVRLVRDIRKRIEEELDYPGHIKVIVI
                     RETRATDYAK"
     gene            complement(9289..10338)
                     /gene="recA"
                     /locus_tag="SCJ86_000852"
     CDS             complement(9289..10338)
                     /gene="recA"
                     /locus_tag="SCJ86_000852"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318847.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase RecA"
                     /protein_id="HEG1168433.1"
                     /translation="MADDRKAALDAALKKIEKSYGKGSIMKLGEKIDQQISTIPSGSL
                     ALDVALGVGGYPRGRIIEVYGPESSGKTTVALHAIAEVQKNGGTAAFIDAEHALDPQY
                     AQKLGVNIDELLLSQPDTGEQGLEIADALVSSGAVDIVVVDSVAALVPRAEIDGEMGD
                     SHVGLQARLMSQALRKLSGSINKTKTIAIFINQIREKVGVMFGNPEITPGGRALKFYA
                     TIRLEVRRAEQLKQGTDIVGNRTKIKVVKNKVAPPFKIAEVDVMYGLGISQEGELLDM
                     AVEKDIVDKSGAWYSYKEDRIGQGRENAKIYMANHPEMMAEVSALVRAAYGIGEEVAV
                     PEDEKGQEELPLVEE"
     gene            complement(10447..11688)
                     /locus_tag="SCJ86_000853"
     CDS             complement(10447..11688)
                     /locus_tag="SCJ86_000853"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016632055.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="competence/damage-inducible protein A"
                     /protein_id="HEG1168434.1"
                     /translation="MKAEIIAVGTELLLGQVVNTNATFLSEQLADLGIEVYYQTVVGD
                     NQQRLEELIALAETRSELILLCGGLGPTEDDLTKEATAAHLGKSLIQNTEGYKKLLAY
                     FETTHRKMTKNNLQQSQIIEGGVPLPNRTGLALGTFYQTDTHAYILLPGPPNELKPMF
                     VEQVRPLLEERFPSEEKLISKVLRFYGIGESRLVTELKDLIETQINPTIAPYAKPNEV
                     TLRLTVKTNDVQAGNQALLALEEKIQERVGEYFYGYGDDNSLAKVVVELLKENKQTVT
                     AAESLTAGAFQAALGDIAGVSEVFPGGFVTYSLQTKAGFLEIDPELLAEYGTVSKECV
                     EQMAIQAREIARTDYSVAFTGVAGPDTLEGQSAGTVWIAISSKDGVVSEKYHFARDRA
                     AIRYNAVMQGFDLLRKQLLRK"
     gene            complement(11928..12506)
                     /gene="pgsA"
                     /locus_tag="SCJ86_000854"
     CDS             complement(11928..12506)
                     /gene="pgsA"
                     /locus_tag="SCJ86_000854"
                     /EC_number="2.7.8.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723896.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-diacylglycerol--glycerol-3-phosphate
                     3-phosphatidyltransferase"
                     /protein_id="HEG1168435.1"
                     /translation="MNLPNKLTVLRICMIPIFILVVGLPLDWGQFNVGDTTLAVTQLV
                     GAVIFALASITDWLDGKIARARGLVTNFGKFADPLADKMLVMTAFIMLVDQGKAPGWV
                     VAIIVCRELAVTGLRLLLVEHGEVMAAAWPGKVKTATQMVAIILLFINNIPFSLIGIP
                     VDQIMLYACLIFTIYSGVDYFIKNSGVFKGSM"
     gene            complement(12715..13638)
                     /locus_tag="SCJ86_000855"
     CDS             complement(12715..13638)
                     /locus_tag="SCJ86_000855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002355331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HEG1168436.1"
                     /translation="MLRRIKQTGEDERVAFETIGEKLRQARLNKKISLDELQQITKIQ
                     KRYLEAIDNDDFDQLPGKFYVRAFIRQYAEAVGEDGDHLVDVFDGKSKLTGSVPERPQ
                     PETVSTSRKAMHQEESHPSRFWTSLPVILLGLVALAIVVVVGYMTWQDRQADPIIGDT
                     ASSLKVEGSVEESSSSEQSTQTKESSTVESTTKSSEEKKMAIATGEDSGSAIAVNITD
                     ATKPVTLEFTAVNRVWLGVLVDNAYVYQGTLAANETQSTVLPETATNATITIGAASNA
                     TIKANGESVPVNPGENNQSPKNVNLTLQYAE"
     gene            complement(14377..15663)
                     /locus_tag="SCJ86_000856"
     CDS             complement(14377..15663)
                     /locus_tag="SCJ86_000856"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287425.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="insulinase family protein"
                     /protein_id="HEG1168437.1"
                     /translation="MNKTEYEQINETLYHEVLPNGLTVYLLPKNDYHKTYGLFSTNYG
                     SIDNEFIPYGEKEKVKVPDGIAHFLEHKLFEKEDGDVFQLFGKQGASANAFTSFTKTS
                     YLFSTTDQVEKNLTTLIDFVQAPYFTEETVNKEKGIIGQEIQMYEDDPNWRMFFGILN
                     NLYPTHPLHIDIAGTVESIDKITAQDLYTCYRTFYQPSNMVLFVVGKMEPEKLMKLIR
                     ENQEAKNFPPKQEIVRYFPENTKEIIKQSALEAAITRDKFVLGIKGLDTLPQEGTELL
                     RYKTAINLLFQMILGNTSRNYLAMYNQGIIDDSFGFEFSLDREFHFADFSGDTDEPEK
                     AAEKVKEIILGFADDPEVSETNLDLLKKKMLGQYFQSLNSIEYIANQFTQSLFGDRTL
                     FDLPEIIDSIQMKDVLAAGEAFISEEAFSEFYMRPK"
     gene            complement(15656..16924)
                     /locus_tag="SCJ86_000857"
     CDS             complement(15656..16924)
                     /locus_tag="SCJ86_000857"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="insulinase family protein"
                     /protein_id="HEG1168438.1"
                     /translation="MVYQLAEGVNLHVLPTKQYKTIRIFIRFTARLQQEVITKRSLLS
                     SMLETNSLNYSDQTKLSAQLAELYGASFGLSVRKKGNLHWLNAGISFVNGEYVNDPNL
                     FSQAVDFLKEVLFYPNIKNHQFDQLTFDLEKNNLRLYLESLKEDKQTFASYALQELYF
                     ENSPEQKIPGLGVVEDLDKITASSLASYYQEMMANDQIDIFVVGDVDPDKAAEAVGQL
                     PFESRETVYPDLFYTQPQVNIVKERQVREPIVQAKLNLAYQTNVYYDEPERFALMVFN
                     GLFGGFPHSKLFMNVREKESLAYYASSSVDTFRGFMSVQTGIDEKNRNQVLHLIHEQL
                     ESLRNGEITELELSQTKAMLRNQYLLSLDSPQAAIEASFLDSWLPETKLSDEEWLKRM
                     ESVTIKEIQQVAEQIELQAIFFLAGGNANE"
     gene            17176..17637
                     /gene="mscL"
                     /locus_tag="SCJ86_000858"
     CDS             17176..17637
                     /gene="mscL"
                     /locus_tag="SCJ86_000858"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373373.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="large conductance mechanosensitive channel
                     protein MscL"
                     /protein_id="HEG1168439.1"
                     /translation="MIKEFKEFIMRGDVLDLAVGVVIGSAFTSIVNQVVQGLITPLIG
                     WFVAMITGTSDLEGALSILDWSPTKGVTFAFGNVISAIITFLITGFVLFLVVKAANRA
                     KNLRSKEEQVEVEETPTAEDYLSDIRDLLAKQVAEDNTHPVEQTNLSDTSK"
     gene            complement(17697..18143)
                     /locus_tag="SCJ86_000859"
     CDS             complement(17697..18143)
                     /locus_tag="SCJ86_000859"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEG1168440.1"
                     /translation="MEYQVKKFEELSTAEFYAIVKERIAVFVVEQQCPYQEVDDIDGE
                     AWHTFFQDTDGKILAYTRVYEEAESIHFGRVLVHQDNRKDGLGRKIVQETINMIQKNF
                     PEKPIVIGAQEYLQAFYESFGFKAISDVYLEDDIPHIDMKKEQVAS"
     gene            complement(18236..18481)
                     /locus_tag="SCJ86_000860"
     CDS             complement(18236..18481)
                     /locus_tag="SCJ86_000860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010750133.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Veg family protein"
                     /protein_id="HEG1168441.1"
                     /translation="MPTTLASIKKDLEGRIGSPIMLVAQTGRKRQTERRGVLTETYPS
                     VFVVDLDPEENSFERVSYSYSDVLTRTVEIEFLTEAV"
     gene            complement(18596..19174)
                     /locus_tag="SCJ86_000861"
     CDS             complement(18596..19174)
                     /locus_tag="SCJ86_000861"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314323.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HEG1168442.1"
                     /translation="MSEELLRAARNGDEEAFAALYQKYFPLLLRLKNTYYLRNYVSED
                     WEQEARIALYQALRTYEKSKGVSFGSYYKSVISNQIYSLLRKQNALKRRDQKDEVSID
                     QKIETEGPDFLAEITLQEPLAIQHLLLKEAIENCEIQFSKIEAKIFHYYMQGVDFVDM
                     AECLAMDHKQVMSAYGRVKLKIKKHLKFYNEE"
     gene            complement(19251..19781)
                     /locus_tag="SCJ86_000862"
     CDS             complement(19251..19781)
                     /locus_tag="SCJ86_000862"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NYN domain-containing protein"
                     /protein_id="HEG1168443.1"
                     /translation="MKKQLLIVDGYNMIGSWPELVQLKKQDKMADAREALLHRLSNYA
                     KYEGMEVIVVFDAQLVPGIQQNYKKYQLDVVFTKEDETADSYIERIAGQLNDRLTQVT
                     VATSDLAEQWMIFSQGALRTSAYELYKTIKKTEKTIAQENKDMHFIDFRRNSPWNEEQ
                     LKKLSQKLDDLMRKNK"
     gene            complement(19785..20645)
                     /gene="rlmB"
                     /locus_tag="SCJ86_000863"
     CDS             complement(19785..20645)
                     /gene="rlmB"
                     /locus_tag="SCJ86_000863"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373371.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA
                     (guanosine(2251)-2'-O)-methyltransferase RlmB"
                     /protein_id="HEG1168444.1"
                     /translation="MGKERTNKHQSKRGFEDKKQNRYSNKKQTGRNQSVSDQNEEALE
                     DNFVFGHHAAVEALQQGRGNKLFLQEDSRGEKVEELKEIAREQAVPVKWVPKQKLETL
                     SDHGVHQGVVLAITPYEYLSLDQLIERTKEKKEDPFFLILDSIEDPHNFGSILRTADA
                     SGVDGVIIPKHRAVGITPIVTKTSTGAVEHVPVARVTNLVQAVQQLKETGFWVFGTDM
                     TGTNFQKWNAKGSIALVIGNEGKGMSPGLKKEMDELLTIPMTGHVQSLNASVAAGLLM
                     YQAFSQRQGE"
     gene            complement(20638..21033)
                     /locus_tag="SCJ86_000864"
     CDS             complement(20638..21033)
                     /locus_tag="SCJ86_000864"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628021.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Mini-ribonuclease 3"
                     /protein_id="HEG1168445.1"
                     /translation="MRDYTQLNGLALAYVGDAIYEIYVRDHLIDQGETKPNKLHRAAT
                     NYVSANAQAFLIQQMLEEHLLTEEEELYYRRGRNAKSHTSAKNADVTTYRVATGFEAL
                     MGYLHLTKQTERMEELIRWCIQKVGEKNG"
     gene            complement(21030..22442)
                     /locus_tag="SCJ86_000865"
     CDS             complement(21030..22442)
                     /locus_tag="SCJ86_000865"
                     /EC_number="6.1.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287445.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine--tRNA ligase"
                     /protein_id="HEG1168446.1"
                     /translation="MIKIYNTLTRKKEEFIPIQKGHVGMYVCGPTVYNYIHIGNARST
                     IAFDTIRRYFEYRGYKVDFVSNFTDVDDKIIRTANELGITAPEVADRFIRAFEEDTKA
                     LNVEPATLHPRVMDHMPDILAFIQVLVEKGYAYESSGDVYYRTRKFENYGKLSDQSID
                     ELEVGASQRTGEEQQQKEDPLDFALWKQAKPGEISWDSPWGKGRPGWHIECSVMATKH
                     LGETIDIHGGGQDLEFPHHENEIAQSEAKTGKKFANYWMHNGYVTIGEDDEKMSKSLG
                     NFITVHEMIKNVDPQVLRFFMATTQYRRPIRYSETTMKEAATNLQRLKNTFDNLKFRQ
                     ETASENLADDADKIALLENLEQRFIEEMDDDFNAANGITVVYELAKWLNTYLDQPKVS
                     AAVLAQGEALFSQWLSIFGILFTAEELLDEEIEQLIEERNQARSNRDFVRSDEIRDLL
                     KEKGILLEDTPQGTRWRREA"
     gene            complement(22439..22975)
                     /gene="cysE"
                     /locus_tag="SCJ86_000866"
     CDS             complement(22439..22975)
                     /gene="cysE"
                     /locus_tag="SCJ86_000866"
                     /EC_number="2.3.1.30"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293094.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine O-acetyltransferase"
                     /protein_id="HEG1168447.1"
                     /translation="MSWFTRAIDAAKRNDPAARSTAEIVLTYPGFHALFWHRFSHFLY
                     QHRLFLLAKINAQFWRFLTGVEIHPGATIGTGVFIDHGMGIVIGETAEIEDDVILFHG
                     VTLGGTGKETGKRHPTVKQGAMLSANAQILGPVTIGKNAKIGAGAVVLKDVPDDATAV
                     GVPAKVVRIKGEKVRRSE"
     misc_binding    complement(23061..23300)
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00230"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="T-box leader; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="tRNA"
                     /db_xref="RFAM:RF00230"
     gene            complement(23297..24757)
                     /locus_tag="SCJ86_000867"
     CDS             complement(23297..24757)
                     /locus_tag="SCJ86_000867"
                     /EC_number="6.1.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287447.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate--tRNA ligase"
                     /protein_id="HEG1168448.1"
                     /translation="MTKVRVRYAPSPTGHLHIGNARTALFNYLFARHNDGDFIIRIED
                     TDQKRNIEDGEKSQLENLAWLGMNWDESPENPGEYGPYRQSERKEIYQPLIDQLLASN
                     RAYKCYCTEEELEAEREAQRARGEMPHYAGTCANLTPEEQAEKEAQGLESVVRFRVPR
                     NTEYAFTDMVKGAISFESDNIGGDFVIQKRDGMPTYNFAVAVDDHLMKITHVLRGDDH
                     IANTPKQLMIYEAFGWTPPTFGHMTLIINSETGKKLSKRDESILQFIEQYRELGYLPD
                     AMFNFIALLGWSPVGEDEIFEHQELIKMFDPKRLSKSPAAFDAKKLQWVNNHYIKAMD
                     LDAFTDMCLPYLIADGRVAENPDEKTIEWVKKIVSLYQPQMSYAAEIVDLSTLFFNEH
                     PVLNEAAKEVLAGETVPTVLHAFKAQLEQMEVVDAPTVKAAIKAVQKETGVKGKNLFM
                     PIRVAVSGQMHGPELPETIELLGREKSLAHLNQVLN"
     gene            complement(24927..26084)
                     /locus_tag="SCJ86_000868"
     CDS             complement(24927..26084)
                     /locus_tag="SCJ86_000868"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400946.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PIN/TRAM domain-containing protein"
                     /protein_id="HEG1168449.1"
                     /translation="MQKRVITLLMIIVGASLGISFLPSAWQAIGQQSNGWLNNTFTNS
                     LLGALIFFLLSLLLAKHISAIIKQVEQKLSEVSLTYLLFGAIGAIIGLTLGVVISTPL
                     YNMEIPFVNSVLPILLMIILGYLGLRMGTTRIEEWKKVFGSRSKKIEQETASQVESQL
                     LERKSGENFHKYKILDTSVIIDGRIYDITKTGFLEGTLMIPNFVLYELQYIADSGDSL
                     KRVRGRRGLDILNALQKEEGISVEMYDGDFEDISEVDSKLIKLAKLLDGVIVTNDYNL
                     NKVSEFQNVPVLNINALANAVKPVVIPGETMNVLVVKAGTERQQGVAYLDDGTMVVVE
                     DGQHYMNERIEVVVTSALQTAAGRMIFAKPAHAGRGIDNKKETEKKTKQSK"
     gene            complement(26118..27488)
                     /gene="radA"
                     /locus_tag="SCJ86_000869"
     CDS             complement(26118..27488)
                     /gene="radA"
                     /locus_tag="SCJ86_000869"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016624348.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RadA"
                     /protein_id="HEG1168450.1"
                     /translation="MAKKSKVQFVCQNCGYVSPKFLGRCPNCGKWNTMVEEIEQDTTD
                     RRTRTSLTGEKAKPTKIADVVPKKEPRIKTKLEELNRVLGGGVVPGSMVLIGGDPGIG
                     KSTLLLQVSQQLAAIGGKVLYVSGEESAEQIKLRAERLGSINTEFYLYAETDMNEISR
                     AIEHISPDYVIIDSIQTMTQPDITSVAGSVSQVRETTAELLKTAKTNGIAIFIVGHVT
                     KEGSIAGPRMLEHMVDTVLYFEGEQHHSFRILRAVKNRFGSTNEIGIFEMHEHGLEEV
                     ANPSQIFLEERLDGATGSAIVVAMEGTRPILVEIQALVTPTMFGNAKRTTTGLDFNRV
                     SLIMAVLEKRAGLLLQNQDAYLKAAGGVKLNEPAIDLAIAVSIASSYKEKGTQPTECF
                     IGEIGLTGEIRRVSHIEQRVKEVQKLGFTKVYLPKNNLGNWEAPKGIEIVGVATLAET
                     LKRVFR"
     gene            complement(27551..28078)
                     /locus_tag="SCJ86_000870"
     CDS             complement(27551..28078)
                     /locus_tag="SCJ86_000870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631526.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dUTP diphosphatase"
                     /protein_id="HEG1168451.1"
                     /translation="MRKRGFEVISKYKDQNINLPQRATHHAAGYDFEAAEEVVIPSIW
                     KAGWKKGLETVADGLKKDVSENEKQLKPTLVPTGIKAYMGEDEFLQLANRSSNPLKRF
                     LVLTNGVGVIDSDYYNNEANEGHIMFQFTNFGFKDVVIKKGERIGQGIFLPFLKADQD
                     QTTAQRQGGFGSSSK"
     gene            28308..29114
                     /gene="proB"
                     /locus_tag="SCJ86_000871"
     CDS             28308..29114
                     /gene="proB"
                     /locus_tag="SCJ86_000871"
                     /EC_number="2.7.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318839.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate 5-kinase"
                     /protein_id="HEG1168452.1"
                     /translation="MRKEIKNAKRIIVKVGTSTLIYPNGNINLAAIDQLAFVLSDLRN
                     RGKEVILVSSGAVGVGMHKLHLSERPTLIPEQQAAAAVGQAELMNIYNQRFSVYNQQT
                     AQILVTRDVVDYPESRKNVRNTLEQLLIMDTIPIVNENDTVAVDELDHLTKFGDNDQL
                     SAIMAKLVAADLLIVLSDIDGFYSDNPTTNPEAQMYRYIDKIDEKILAQAGGAGSTFG
                     TGGMQSKLKAASRIFEANSAMILANGKNPAIIFDILAGKEVGTLFKEDLK"
     gene            29111..30358
                     /locus_tag="SCJ86_000872"
     CDS             29111..30358
                     /locus_tag="SCJ86_000872"
                     /EC_number="1.2.1.41"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293098.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate-5-semialdehyde dehydrogenase"
                     /protein_id="HEG1168453.1"
                     /translation="MTDLMQLGQQAKDSAQQLALMPTNRKNELLVQMAQTIKKNQQAI
                     IEANHKDLEKAVENNISETMQDRLQLTPERINAMATEIEKIASLDDPIGKVDEMWTNT
                     DGLRIGKKRVPLGVIGIIYESRPNVTTDAASLAFKSGNAVILRGGKEAFFSNQLLVQL
                     LQQVLVSAGESPYAIQFVDDTSHETAGQLMRLTEYLDVLIPRGGAKLIQRVKEQATVP
                     IIETGTGNNHVYIDKEAQLSMAVNIIVNAKASRPSVCNAAETLLIHSEIAPFFLPAIE
                     KELVEHGVSLRADARALEYLETAALAEDTDWDTEYLDYILAVKVVDSLDEAIAHINRH
                     NTKHSETIVTDSYAASQRFLNEVDAAAVYVNASTRFTDGSVFGFGAEIGISTQKLHAR
                     GPMGLNELTSTKYIIYGDGQIRE"
     gene            30434..30778
                     /locus_tag="SCJ86_000873"
     CDS             30434..30778
                     /locus_tag="SCJ86_000873"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEG1168454.1"
                     /translation="MFEILMIFFIYLISLNIAAFLGVGILSLFFQFKKRSIGSQREKW
                     SQYFDKIGQKGLVTRLHISYMVALCLLATINYYSFFDHSIAYTITLLIAGIFHLSYKY
                     QLNKNHLNRTFR"
     gene            complement(30895..31221)
                     /locus_tag="SCJ86_000874"
     CDS             complement(30895..31221)
                     /locus_tag="SCJ86_000874"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008266141.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEG1168455.1"
                     /translation="MLEEYFQEVGLTLVELPIEFSDDRLIYRGYIKELPIINAEGETR
                     KAMQQKIAGMYQVYREQLLAESAEEEKEKTMNLSMDELLRYYDGETFDGFSLLNEEET
                     DLPGSW"
     gene            complement(31836..33038)
                     /locus_tag="SCJ86_000875"
     CDS             complement(31836..33038)
                     /locus_tag="SCJ86_000875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEG1168456.1"
                     /translation="MEILENQIDIQEQTNEETKGKSSALVVAATCMIAFMGVGLVDPI
                     LKTIAAQLNATPAETTLLFTSYMLVTGVVMLFTGFLSSRIGLKKTLMTGLFIIVVFAG
                     LGGLSGNIGMLIGLRAGWGVGNALFVSTALATIVSILVGNTEKAIMMYEAALGCGMAV
                     GPLVGGVLGSGSWRYPFFGVAFLMFCGFIALAVLLPETEKPKRKVGLWEGVKALSNHK
                     LRSVGLIALLYNFGFFTLLAYAPFLLIGYSELEVGFVFFGWGVLLAIASIFVAPALER
                     KFTTYRTILAAFVLFIICLLLLGAGTSMPVLVPISIVLAGFFQGIINTLLTTIAMEIP
                     GLQRNVASSSYSFVRFFGGALAPFIAGVIGEHIGESYAFYFAGAIVLISVCCIIYHRN
                     YFATNEEE"
     gene            33155..33670
                     /locus_tag="SCJ86_000876"
     CDS             33155..33670
                     /locus_tag="SCJ86_000876"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629798.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PadR family transcriptional regulator"
                     /protein_id="HEG1168457.1"
                     /translation="MNTLAYILLCVLLSKPRSGYELKQLITIFWEAHHSQIYTTLAKL
                     DQQGYVTVLDKDEHSQKKIYHLTDEGRALVEEWIKEETPAPTQKDEFLAKIYAFSTLD
                     KTTATGLLFTREQQLNKIMQKNQKKLNDLSLDKKQEFGRYVVIQRRIMLCQQELQWCQ
                     QVREQMQQFFE"
     gene            complement(33869..34315)
                     /locus_tag="SCJ86_000877"
     CDS             complement(33869..34315)
                     /locus_tag="SCJ86_000877"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328790.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2798 domain-containing protein"
                     /protein_id="HEG1168458.1"
                     /translation="MPTNKKEGIIFTTMMCFLMVLGMSAYNLWLHQDLHLSSLLIGLV
                     PGFVVAFILDVFIIGVLAKKIAFKLPVNPESKVQMILTISCLMVLGMVTCMSLFGVIM
                     EQGIPDQLFSAYMNAWKMNVIAALPLQLIIVGPLSRSVLKAIQSNE"
     gene            complement(34362..35840)
                     /gene="msr(C)"
                     /locus_tag="SCJ86_000878"
     CDS             complement(34362..35840)
                     /gene="msr(C)"
                     /locus_tag="SCJ86_000878"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314314.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F type ribosomal protection protein Msr(C)"
                     /protein_id="HEG1168459.1"
                     /translation="MENLAVNITNLQVSFGNQLELSIDSLRVYQQDRIGIIGENGVGK
                     STLLKLIAGELFPDHGKIQTEITFNYLPQLTYLAEAKDLNLELASHFQLRLEETSERK
                     WSGGEERKIELIRLLSSYEQGMLLDEPTTHLDRKSIDRLIEELRYYYGTLVFVSHDRY
                     FLDELASKIWEVKDGEIREFSGNYSAYLTQKELEKKTQLREAESIMKEKKRLEKSIQE
                     KKKQAEKLEKVSSKKKKQQIRLDRLSSSKQKDSVQKAIQKNAKTLERRLQKIGETTKP
                     QQMKQIRFPVPKSLELHSRYPIMGQNVQLERSGRTLLVNGDFQFSLGKKIAIVGENGS
                     GKTTLLEHIRKQGEGILLSPKVSFQVYQQKGYQMTSEESIIRFVMRQTEFSESLVRSL
                     LNHLGFAQETLTKPLCTLSGGEATRLTIALLFTKPSNVLLLDEPTNFIDMATIEALEK
                     LMQIYPGTILFTSHDSYFVERTADEVYEIKGQKIKKVLTRNF"
     gene            complement(36194..36823)
                     /locus_tag="SCJ86_000879"
     CDS             complement(36194..36823)
                     /locus_tag="SCJ86_000879"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287459.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitroreductase family protein"
                     /protein_id="HEG1168460.1"
                     /translation="MEQKNDFSTIMTGRRSVRVYDETYKIPHEEMLEMIKEATTAPSS
                     VNMKPWRFVVAESEEAKEILRPLIRFNTRQNDTSSAMVMIFGDMQCYEYGEEIYNQAY
                     ESGKMPKEVRDQQLAAIIPYYKSFSREEMNDVVKVDSSLAAMQFMLVARDHGYETNPI
                     GGFEADQLAEAFGLDKDRYVPVIILSIGKAVEEGYESVRLAPEKITMFE"
     gene            complement(36807..37271)
                     /locus_tag="SCJ86_000880"
     CDS             complement(36807..37271)
                     /locus_tag="SCJ86_000880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373333.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HEG1168461.1"
                     /translation="MSLEEISHLLYQIKLADQKIVQIFEENIGISLTRYEIMMRLKEE
                     NPLLQSSLQEKLQIDQGAVTRHLKVLEANGYVSRQRNPQNNREVYVALTEKAQKKIKH
                     CEMDHKDTGSVLSPAFSDVEMKQFLTLLDKLNTQLCNITTETKEVNNNGTKK"
     gene            complement(37758..39026)
                     /locus_tag="SCJ86_000881"
     CDS             complement(37758..39026)
                     /locus_tag="SCJ86_000881"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 73 protein"
                     /protein_id="HEG1168462.1"
                     /translation="MKAKNLLFSVILLGNLAVANTTVLAEESTETVATQTSSSSETGI
                     ENDTESTLQSATVSENTISTEEPTTETTEQTQTEDSTSAEAQNNPENAEEITNQLTDG
                     QTLTHTHQADLSEFENYTGYAFYARSSANSQQAFIDSIASTAQSLASANDLYASVMIA
                     QAIVESGWGNSTLASAPNYNLFGIKGSYNGQSVTMPTSEYVNGQWITVNAAFRKYPSY
                     KESLQDNVTVLKTTSFQPGVYYYSGAWKSNTNSYKDATAWLTGRYATAPNYGSTLNNV
                     IETYNLTQYDTAPTSTSPMYRLYNRHTGEHLYTLNAGEKNYLPTVGWEYEGIAWQAPN
                     SGQPVYRLYNPYSGDHHYTMAQSEINSLTKIGWRYEGLSFYSGGSKPIYRLFNPNEKT
                     GTHHYTLSAKERDWLTPMGWRYEGIGFYGY"
     gene            complement(39257..39994)
                     /locus_tag="SCJ86_000882"
     CDS             complement(39257..39994)
                     /locus_tag="SCJ86_000882"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325513.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HEG1168463.1"
                     /translation="MNDLSEKVIVIMGASSGIGEATTKLLAEKGAKLVIAARREDRLK
                     AIKESLPEAELYIQTADVRDFAQVQAVIDLAMEKFGRIDVLYNNAGIMPTAPLVEGHR
                     DEWQNMLDINIMGVLNGISAVLPIMEKQKSGHIISTDSVAGHVVYPDSAVYCGTKFAV
                     RAIMEGLRQEQRQNNIKSTIISPGAVQTELYQTISDKKAALELHEAQKEWGLTSEDIA
                     SAVAFAIETPDRMSVSNMIIRPTTQEV"
     gene            complement(40061..40630)
                     /locus_tag="SCJ86_000883"
     CDS             complement(40061..40630)
                     /locus_tag="SCJ86_000883"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar O-acetyltransferase"
                     /protein_id="HEG1168464.1"
                     /translation="MTSLIEKQIVNKEIRVTDPLFEVIHQIQAENEEPLAALNTGYHE
                     PADIRKRLENIISDKVDDTVTVLLPFYTDFGKHISIGKNVFINRQVMFVDLGGICLED
                     SVLIGPRVNLITVNHLTDPKERRGLSVKPIHIKKNAWIGAGATILPGVTIGENAIVAA
                     NATVTKDVPDNTIVAGIPAKIVKPVERII"
     gene            40758..41630
                     /locus_tag="SCJ86_000884"
     CDS             40758..41630
                     /locus_tag="SCJ86_000884"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629796.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HEG1168465.1"
                     /translation="MDIRILTYFLAVAREKNISRAAKSLHITQPTLSKQLKDLEEELG
                     VTLFTRGSREIQLTDSGRYLYSKGKEILSLVEKTANNLTNDAIISGEIYIGGGETKAI
                     SFISDSLHSLIKAHPDIQFHLYSGNADDIIDKLDSGLLDFGLVIEPTNKQKYQSFRLP
                     AQDRWGLLIHRNHPLAKQTVIHPQELKTVPLIISRQTSVDSQLAEWLGFSIDQLSIVG
                     TYNLLYNASLMVDNGTFGALALDGIINTYGTEKVFIPLDPPLQASLNLIWKKDQPLSP
                     AARKFLDVLTEKYS"
     gene            41972..42844
                     /locus_tag="SCJ86_000885"
     CDS             41972..42844
                     /locus_tag="SCJ86_000885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005874851.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PRD domain-containing protein"
                     /protein_id="HEG1168466.1"
                     /translation="MKIKKIFNQNAVLVDDGGQEKVAIGKGIGFDRKRNDLIFDRDVE
                     RIFVMEPEGQIKLQNLLNQIDERFLFAAETIIDHAETVLMEKLNEHVLIALTDHIAFS
                     AENINNGIVIRNKLLREIEVLYSDEFSIAQWAVEYLTKELGIPYTYDEAGYIAIHIHS
                     GRSGRNNNHRSIREVTIVSDIIHLVESELETDIHSEPFSLNYSRLVNHLRLLLQRTHA
                     QQYAVLDTEIVEMVKRKYPESYKISKKIRVLLTKEYQLAITKEELGYLAIHIERLRSA
                     IVQTNVNNHEEEKN"
     gene            42841..44799
                     /locus_tag="SCJ86_000886"
     CDS             42841..44799
                     /locus_tag="SCJ86_000886"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335294.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HEG1168467.1"
                     /translation="MKAYLQRMGRSLQLPVAVLPAAALLVGIGNWWASYSNDIVAHFL
                     QAGGNAVLGQLPLLFAVGLALGMSVDKSGAAALAGLVAFEVPVNVLKTDSVATLLNIK
                     PESVDPSFAQISNVFIGIIAGLIAAALYNRFHEAKLPMALSFFSGKRLVPILAAFVML
                     LISAVLLLVWPPVYSALVSFAKFILSLGWGGAGLYGFFNRLLIPTGLHQAMNSVFWFD
                     VAGINDIGKFLASEGTKGVTGMYQAGFFPIMMFGLPAGAYAIYRNARPENKAKTASLM
                     LAAAFASFFTGVTEPLEFSFMFVAWPLYVLHAIFTGISLAVSAFFHWTAGFAFSAGFV
                     DYFLSLKNPIANQPLMLIVQGLVTAVIYYFGFNFAIKKFHLMTPGREEADLSDDDTAT
                     TNSDNKYAAQASKIYAALGGADNVTSIDNCTTRLRLQVKDTGLIDQNKIKATGVPGMK
                     IIDGKNAQVIVGTEVQFVADEMAKLHGGAATRPAQTNTVVKTETFAGGTIEQDIYAIA
                     NGKLIPITEVSDDVFAEKMMGDGYAVLPENGEIFSPIAGTITNIFPTKHAVGIQTDAG
                     IEVLLHMGINTVDLKGEPFTLYVKEGQKVARGQLIALVDLAAIQSAGKNTDMVVVFTN
                     GDKVQSLEIEPARDVKANDKIGSVSNKA"
     gene            complement(45131..45982)
                     /locus_tag="SCJ86_000887"
     CDS             complement(45131..45982)
                     /locus_tag="SCJ86_000887"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HEG1168468.1"
                     /translation="MHYLVTGATGGFGGYALEFLKKMVPQNEIYVLVRSEEKGEALKE
                     AGFNIRIGDYDDLDSMKEALKGIDRLLFVSGVPGNRQAEHKNVVKAAQAAGVSYIAYT
                     SFADADHSISVLAPDHQFTEKMIKDSGISHTFLRNNWYVENELPIIGQALKTGQFVYA
                     AGNGKTGWALKREYAEAAAKVLVESDSPEILELSGNLTDYEELAKALERATGKKLEII
                     EASDAAFVENLKEAGFPQEAADMFLAIQHDIKKDQLAISSDDLEKVLGKPLTSLEAAL
                     KEILDQE"
     gene            46105..46572
                     /locus_tag="SCJ86_000888"
     CDS             46105..46572
                     /locus_tag="SCJ86_000888"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332248.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rrf2 family transcriptional regulator"
                     /protein_id="HEG1168469.1"
                     /translation="MKYSLQFSDAIHVLAYIEMFQDTRSLSSEMIASSIETNAANVRK
                     IMSQLKKSGLIFTKTGKPQPSLAKSPDQISLLDVYRSIEGNTQLIQVDPKTNPNCVVG
                     ANIQEALEESYARLQKKAEEEMAKITLDSLIHKIAVLEKEKRPENTGLVERFL"
     gene            complement(46835..47317)
                     /locus_tag="SCJ86_000889"
     CDS             complement(46835..47317)
                     /locus_tag="SCJ86_000889"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="effector binding domain-containing protein"
                     /protein_id="HEG1168470.1"
                     /translation="MAKYRIEEKPDFTVTGIGVELKSSNTDFVGLAAEKQAFEQKVIG
                     NGTIDALKQAAENHYFFTVNEPYNGKMMYYVGVQTSQDVPNTTRIIPFPKGTYLVVIA
                     LAESPEELRNDLTNTAFGHVLREVEDYAYVGGPNAVVEMGVTNGKYMGEIWIPVVNKP
                     "
     gene            complement(47417..48280)
                     /locus_tag="SCJ86_000890"
     CDS             complement(47417..48280)
                     /locus_tag="SCJ86_000890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326092.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
                     /protein_id="HEG1168471.1"
                     /translation="MLKDLNQLIDYIEEHLTEGLSYKIIAENTGISEYQLKRIFTFIA
                     GMSLMDYIKNRRLALANRDLLDGSRVTETAFRYGYETVEGFSRAFRDWSGYLPSEISR
                     YGIQKSFPRLTFFIDVRGGKSMEFRIEKKEQFYVAGVAKKVPVKFEGVNQEIQKLAQT
                     ITETQREEMHKLGDLYPNQVINVSYGFDGARLEEKGSLMHMIGFATTKENPYADLEML
                     AIEEHTWAIFPNKGPFPQTLQDTWGKIYAEWLPSSDYELVEAPEISFTKWGEDFSNVY
                     SEIWIAVKAKK"
BASE COUNT        13906 a        10426 c         8691 g        15663 t
ORIGIN      
        1 ttttcgtttt tataaggctt cttttcttta taatttagtt attttttctt ctgaaggcaa
       61 ccaatattcg tttgatgata tttacaatca ggaacaaagc aataaagact aatgtgattg
      121 ttgctcctgg aggtgtatct agataaaacg agctagttag tcctgacagc atccctagaa
      181 atcctacaca aacactgata gcaatcacga cattgaagct tttgcctaat cgcataccga
      241 ttgctgcggg aagaaccatg attgcggata tcaaaagtgc accggcaatc gggatcatca
      301 cagcaatagc cacaccagta atcacattga acatcatcga catcaaatga acgggtagcc
      361 catccacgtg ggcggtatct tcatcaaaag tcagaacata catcggccgt ttgaataaaa
      421 agaacaatat accgatggca acgaataaga ttcctaaaaa gatgacttgc tcccatgtaa
      481 tggtcacgat cgaaccaaac agataggatt ggatacttgt tgctgaattc cctccgctca
      541 aattcatcaa tactaacgct agcgccaaac caccagccat taaaatggca atggagattt
      601 ctgaataggt tcggtagatc gttcgcagat actccaatat cacagctgct aatacaacga
      661 ctagcatggt cgtaagtgta ggattaaggt tcaaaaagaa tcctaacgct acccctgcta
      721 aagagacatg agaaagcgta tcagccatca gcgattgtct tctgatcact aaaaacacac
      781 ctagcatcgg ggcgattccg gcaatgaaga tagcggcaag aaacgctctt ctcatgaatt
      841 catatgaaag caacgccatt gagaatcctc ctttctcacc agtcggatct gtctgtccgc
      901 atactgtttg atatcttcat gatcatgggt gatcattaga actgatttcc catggacatg
      961 cgcactatgt cggagaagac gataaaattc atttctggat tcttcatcca tacctgttgt
     1021 tggttcatca agaacaaata agtcagggtc agtagcaaaa atacgcgcta aactgattcg
     1081 ctgcttttgt ccgccagaca attcacctat tcgccgattt cgcatatccc acatccctac
     1141 agcttctaaa gcacgctgta catgttgatg atctttttca gagagacgct tgaaccagcg
     1201 gcctcttgga tatcttcctg aacggactaa ttcgatcacc gtactaggaa aacctgcatt
     1261 gaaagatgca acttgttgag gaatataacc gatacttaat ttttttcctt cttgattttt
     1321 ctcagcaatc tttatcattc cttttgaagg tttcagtaat cctaacgatg ctttcaccaa
     1381 ggttgacttg gctgctccat tttctccagt taaaatgaca aattcacccg catctacatg
     1441 ataagagaca tcttctagta caggttcatc atcatagtaa aaggtcaaat cagatatttc
     1501 gatataacgc accttacaac ctccattcta aaacttaatt gatactttct tttaaagctt
     1561 tcagattttt ttccatgaca gaaatataat tttcgcctgc tttgatttgt tcattagtca
     1621 agctttctaa aggattcaac acttctagct tcactcctgt ttcattcgac aatgtctcag
     1681 ctaccttcga agaggcattt tcttcaaaat aaatcacttt gatgttgttt ttcttcacaa
     1741 attcttttag ctcagctaag cgacttggtg tgggttcctg atctggtgaa acgcccgaaa
     1801 tcgctacttg ttttaagtcg tattgattcg ctaaataagc aaaagcagca tgctgagtga
     1861 caaatgtgcg gtttgttgca tcttttagct catcggagta tttttgatcc agtgcttcaa
     1921 gctttttgat ataagcatcg gcattcttcg taaaagtttc ctttttatct ggatattttt
     1981 cgcctaattc ttcagcaatc gttctgactt cttttatagc taataccgga tcgagccaaa
     2041 catgtggatc gtattcatgt gaatgaccat gcccttcttc tgattcttca tgttcgtctg
     2101 cttcttgcga ttctaaacgg tcgattcctt tagctgcttc gatcatcaag gtctgtttgc
     2161 tatccactga atcctttaac gaatccacaa aaaattccat gtctgaacta ttatacacga
     2221 aaacatctgc atcggaaatt tctgctcttt cttttgcact tggttcgaaa tcatgcggct
     2281 ccgttccagc agggatcaaa agtttcacgt ttccttcatc ccctacgatc tcttttgtaa
     2341 aatcatacat cggataaaaa gtcgtaacga tcgtcaaatc ctcttttttg tctgcttcct
     2401 tgttcgtatt tccacatgct gcaaacagta aagcacctat cattaccgta gctgtcaaaa
     2461 gatatttttt catttatatg ctcctttcaa tgaacaaatc ggaattttac gatttattaa
     2521 cgtgaactaa tgtatcaaaa taaaaaaaca cagtcaatag aaatccatca atttctgatt
     2581 taagcatgta tattcttgat taaatcgtaa aatggatgag ttgctgctca tatctagcca
     2641 tttcactaca ataaaaaact gaagaatacc attttaggat tcctcagttt ttcatcacct
     2701 attttacttc ttatatttta ttgcagggtt cgtactaaat aaacttcctc acaaaaacct
     2761 tttaacccat tatatactct ctgcgctctt gaataatgtt gacacaaggc aaaaacggtt
     2821 ggtccgcttc cactcatcaa tgcagcatct gctccgtact tgatcatacg ctctttgatt
     2881 tgatggatga ctggatgccg ctttcccgtt atatcttcta aactgtttcc catttttttg
     2941 atcatctgct gataatcttg ctctcggatg gcttcagcaa gtccgtggat gtcttgatgt
     3001 tggatggctt gcgtatctac ttctctaaac actgtacgtg tagagacact cattcttggt
     3061 ttgaccaaca caacccagca ctgtggcata gaaggtaata cttggatttc ttcccctttg
     3121 ccaccgacaa atgccgtact tccgtagaca cagtaaggaa catctgtacc gacttccaaa
     3181 ccgatttctg ccagttcttc taacgaacag cccagattcc ataattggtt gatccctctt
     3241 agagcagctg cacagtctgt acttcctccg cctaatccag ctgctactgg aatattcttc
     3301 ttaacaaaga tcttgacacc ggttttgata ttgtatttat tttttagcaa taaagcagct
     3361 tgataaacat tgttccgttc atctaccggt aaaaatgctt tatttgtttc tacaatgatt
     3421 ttgtcttctg ttatttcttc aaaaaataga tgatctgcca agtctacgct cgacatcacc
     3481 atttccaact catgataccc atcttgccgt ctatataata catccagtcc taaatttatc
     3541 ttggctggcg ctttttcaat caattccatc ctctcaccca ttcgaaaagt ctaagtcgca
     3601 agtattctat catagggaaa aataaaagta tagcttatgt ctgcctagtg gttgattttg
     3661 tcatcgtttc aacagctttc acagaatttt ctcttcataa tccaaacaag ttctagtgat
     3721 tcttataatt ctgtgagcac ttcttctgcc aaactcggaa catttccttt ttcttttcct
     3781 atcagactga gtagctgcat ggtagcaaat ccgaaattac ttcctttcct ataagcaaaa
     3841 taacgtcctt cccaatgatc gacatatttt aatttctttt cataagcaga ctggaaggaa
     3901 tagaaaaaat taccataatt atatacgata tgcatgactt tttcttgaaa gaaagaaaaa
     3961 tctgtttctc caacattagc taatggagct aagcctagat tggcttccga gtagcccatt
     4021 ttctcgtata caataaacag attggctaac agatagtcag ctaattcctc agattcttta
     4081 ggtaagaccc gcaacaaatc atatcccgcc tgcttcttcg tcatcggctg agcggtgatg
     4141 aagccaacga ctttttgatc tttacggaca ataccgacac cgctagataa tacatatgat
     4201 ttatcaaatc gtcccacaga gaagtatttt tctcgtgcac cttgaagcca tttttgtgaa
     4261 acttcattta cagcttgatg taattcttcc ggcagttgat cgtaccaagt aaaatggtat
     4321 ccttctttca tcaaacgttg atattcaagt tgctgactca gccgagacaa gtcttctgtt
     4381 gctagatcaa caatccctgc ttcacctact ttcgtgaagt gaaaacccaa ttcatgaagc
     4441 atcactacgt agctttctga tatcttataa aacgcaagct gatagcctaa acaatctgct
     4501 tgtttcataa actgattcgt tgctgcccgc atcattttag gattgccaac tggatctcct
     4561 aaaacaaagc atttgtttcc tttgatctga taaccaaaaa cgacttgatc ttggtcgtct
     4621 tcttggtaat aaaaataact gtaacctgac aagcgaagct gatggcttgt agctgtgcct
     4681 ccgtatgttt ccaataaacg gtcaaaacgg tctttttcgt actcactgcc aagcttcgta
     4741 gtatcttcag ctaaatattg ataaagcgcc actaaagcta gcaatgagat gcccaaacca
     4801 ataagccctg agaaccacac atcatctgaa ggaaacaaaa taaaatgatt tcctgttatt
     4861 cgaccgacta aaaagcgtcc gctgtgatag cctgcaattg cgtaaacgat aaacaaaaag
     4921 ccgaacaagc tagcatcaaa gattatagct ccccaagaat aaacgaattt tgttcgataa
     4981 aattcttttc tggataacca cacagctaac agaatcaaga catataccaa tatcaactgc
     5041 caagataacg tacgtgtgat tgtatttaaa ataccaaaag atagtaaagc aatcgtaggc
     5101 agaaatgcct ttttgacttt tactgaaagt gctctagcaa ggccgagtaa taaaaagcca
     5161 accaatagat tcactgtctg atctaaaaaa ttgaatgaga acggcaataa tacttgaaat
     5221 agccgactga ttgcagacag attagtgatt gttgatagaa gtaccatcat gatccccgca
     5281 aaatagacag cacttacgag cacgatatgc gcaacctttt ggaaaaacag tcgcggaatg
     5341 ttatctaaaa atcgattgat tttgactcct gtatgtgtga tgaacaatag aagtcccgtc
     5401 ataaaaggta gaacataata aaacaaccga taatataata gccaaactac tgctacatct
     5461 tgccgaacag acaattgact cattcctaag atcatcaaaa cgtcaaaagt ccccattcca
     5521 cctgggacca tcgtcagcat tcctatcaat gtagcaatca tgaacatagg atagatcgaa
     5581 agcatcgaaa tatctattct catcaacttg ccgatgatca aaaataccgc aagggctccc
     5641 gtccattgtc caaaggatgc cagtagcaga ccaagcaagc cttttggata aaactcgaag
     5701 aacaatgtct ttctctttag ataagcaaaa aagaaaatga ctggtgctaa aaaactaccg
     5761 ccgaaaagcc agatccaata gcttcgaaaa atactttctg actgaataaa aaagacatca
     5821 ataaatgtaa gaaacgacca tacagacaaa ccagtcagca taaatagtgc gacttttgat
     5881 acagtggcta aaaccatttt tctatttgtc tgctttccat aaaaactagc ccgcagactt
     5941 gctcctacca caccaccaaa accggcgaga ttgttgatcg tgtttgttac ccacgcagca
     6001 agaagaaact cttttctttc catttttggt tttccttgtt tttctaagac tctgatcgtc
     6061 acccaatcat acaacaacat cggagtgaca cccagcagtc cagccagaat catgagcagg
     6121 atcgtcatgc ggctttgttg tcccatcctt tgccaaatat cttgccaact catgccatta
     6181 gcaatatttg cgacttgatt tgccacaaag actaacacgg agccgaaaaa aatcagtttt
     6241 aaaatcagac tatgttcctt tatccattgc agcatcaagt atcgtttatt tttcacattt
     6301 tcccattcct aacttgtata cttttgtgtc ataccccaat gttatcttat ttattttaat
     6361 ttttccactt tagacctgtt ctttgcttgc gatgatagaa aaagaaagat atcctaatgc
     6421 taaaggagag gataacatga aagtaggatt tatcggcaca gggaatatgg cacaagctat
     6481 tattgatggt ttattaaaaa aaggaacagt ctcgaaagaa gaggtgttcg tttccagtgg
     6541 acattttgaa aatgcacaaa aatttgctgc tgaaactggc gtccatgcat gcaaaagtaa
     6601 tcaggaagtt gcatcacaat cagatatcgt tcttttagcc gtgaaaccaa aaatcattga
     6661 aaaagtattg gaagaactaa gagaatatag ccagaaagtc ttattcattt ctattgcttc
     6721 aggaaaatca ttaatagatt tagaaacagc gcttgaagat cctcaggcct ccatcgtacg
     6781 tgtgatgccg aatgtcaatg tcagcgtagg agcaggcgtt tctgcgattt gcaaaaatga
     6841 agctgccacg gaggaacagc ttcagcaagc tacgatgatt tttgaagcaa tcggcagcgt
     6901 ctatccttta gcagaatcag atttcagtac attcattggc cttgctggca gctcacccgc
     6961 attcgtttac atgctgatcg atgcgatggg acggactggt gtactgaatg gtttgtccaa
     7021 acagacagcc actgagattg ctgcaaaagc aattatgggt tcttgtcaaa aattcctttc
     7081 aagtgataaa aatccatggg aacttgtaga ccaagtttct tctcctggcg gtacaactgt
     7141 tgcaggtgta gttgccttag aagaagcagg attcatccca gcaatcatca aaggaatcaa
     7201 tacgacgatc gagaaagatc ttgagatgtt gaataaaaaa taatgatagc aatgtttttt
     7261 cgtgccttac aaaaaaagaa gagctgtggc ttttgccgcg gctcttcttt tgtatttttt
     7321 attatttagc ataatcagtt gcacgagttt cccgaataac gattactttg atgtgtccag
     7381 gataatccag ttcttcttcg attcgtttac gaatatcacg tactaaacga acagcgtcta
     7441 aatccgagat ttcttctggc ttgaccatta cccgaacttc gcgtccagct tgaactgcgt
     7501 aacttgattc tacaccggcg aagctgttcg aaatattttc cagacttctt aaacgacgga
     7561 tgtagttttc taatgattca cttcgcgcac caggtcttgc tgctgagagc gcatctgctg
     7621 cagctacaag gacagagata acagaagtag cttccacatc tccatgatga gaagcgatcg
     7681 cgttgattac tactgggttt tctttgtact tggcagctaa ttccgcaccg atctcaacgt
     7741 gagatccttc aatctcatgg tcaagtgctt tcccaatatc atggagcaac ccggcacgtt
     7801 ttgctaattg gatatcttcg ccaagctctg ctgctaagat accagctaat tttgcaacct
     7861 cgaccgaatg cttcaagacg ttttgtccat aactagttct gaatcgcaga cgtccgatga
     7921 tcttgattag atctggatga agtgtatgag caccgacttc aaacgctgcc tcttccccgt
     7981 attcgcgaat acgttcatcc atttctttac gagatttctc gaccatttct tcgatacgcg
     8041 ctggatggat tcgtccatct tgtatcagtt tttccaaggt catccgtgcg atttctcttc
     8101 ggattggatc gaatccggaa agaacaactg cttccggtgt atcatcgatg attaaatcga
     8161 taccagtcag cgtctcaagc gttcgaatat tacgaccttc tcgtccaatg atacgacctt
     8221 tcatttcatc attcggaagg gtaacgactg aaacagtcgt ttctgaaact tgatctgctg
     8281 cgcagcgttg aatcgctaat gataataagt ttttcgcttt gcgatctgct tcttctttgg
     8341 cacgctgttc agattctttt accattagag ttaactcatg attcagctct tcttcagtcg
     8401 atttcatgat gacatctcgt gcgtcctctt ttgataaacc agcaattttt tctagttctt
     8461 tttgttgatc ttcaatcatt ttttcaacgt ctttttctcg ctcttcaatt aattgctgtt
     8521 ttgaactaag tgcttcttct ttactttcaa gtgagttctc gcgtttttct aacgagtcat
     8581 ctttacgatt cagcgtttgt tcgcgttgaa tcaaacgatt ctcttgagat tttaattcta
     8641 acttactttc tctcagctca ctttcaactt cagaacggta tttctgattt tcttccttag
     8701 cttctaacaa tgcttctttt tttagagttt ctgcttcttt ttttgcacta tcgatgattc
     8761 cagcagctga tgattgtgca ccagctattt ctttctcgtg acgtgacttt gcgaccataa
     8821 accctaaacc aagaccgaca attaaaccga tgatagcgag gagaatgttg aataccataa
     8881 aatccacctc catactatct ttttcatttt tatcatctat acagcttcta tgataaatta
     8941 ttctaaactc gtcaatatgc atcttcgcat atatgtgtac aaaaaacatg cacaacttta
     9001 ttctaatgtt tgtaccccgt agtgtcaact ttaaataaac aaagaaacgg ctttctactt
     9061 attcattaaa cgatggtttt taaaataaat gacactagtt tcatataggt aaacctttaa
     9121 attgagaaac ttttctatta aattattctt tgtagatgaa cagatatttt tattttatgt
     9181 acccactcgt ataattaaat aactcacgat ttctttattc aataaaaaaa cggcaggtca
     9241 aggagagtac ctactcttca gaccagccgt ttttgtttat cataattttt attcttctac
     9301 taaaggcaat tcttcttgtc ctttttcatc ttctgggaca gcgacttctt caccaatacc
     9361 ataagctgct ctaactagag cagaaacttc tgccatcatt tcaggatgat tagccatata
     9421 gattttagcg ttttcccgtc cttgaccaat ccgatcttct ttataagaat accaagcacc
     9481 gcttttatca acaatgtctt tttctactgc catgtctaga agttctcctt cttgtgagat
     9541 ccctaaacca tacatgacat ctacttcagc gattttgaaa ggaggtgcta ctttgttttt
     9601 tacgactttg atctttgtac ggttaccaac aatatctgta ccttgtttca attgttctgc
     9661 acgacgtact tccaaacgga tcgttgcgta gaatttcaat gcgcgtcctc caggagtgat
     9721 ttctggattc ccaaacatca cgcctacttt ttcacggatt tggttgatga aaatagcgat
     9781 tgtttttgtt ttgttgatcg aaccagagag tttacgcaat gcttgagaca tcaaacgtgc
     9841 ttgtaacccg acatgtgagt cacccatttc gccgtcgatt tctgctcgtg gaactaaagc
     9901 agcaactgaa tcgactacta cgatatctac agccccactt gatactaaag catcagcgat
     9961 ctctagacct tgttctcctg tgtcaggctg tgaaagaagt agttcatcga tattcacacc
    10021 taatttttgt gcatattgcg gatctaacgc atgctcagca tcaatgaaag cggccgttcc
    10081 gccatttttt tgtacttctg caatagcgtg tagtgcaact gttgttttac ctgaactttc
    10141 aggtccatat acttcgatga tacgtccacg ggggtaaccg ccgacaccta aagcgacatc
    10201 taacgcaaga gagccacttg gaattgtaga gatttgttgg tcgatttttt cgcccaattt
    10261 cataatagag cctttaccat agctcttttc aatttttttc agtgcagcat ctaaggctgc
    10321 tttacgatca tctgccaata cgataatcct ccttatattt acaaatcaga gcaacatttc
    10381 attatcctta tcatatcttt tttagtgaaa aaaagcaaga aaaaaccgaa caaatattcg
    10441 catttatcat tttcttaaaa gctgttttct caaaagatca aatccttgca taaccgcatt
    10501 atatctgata gcagcacgat ctctggcaaa gtgatatttt tccgaaacca cgccgtcttt
    10561 agatgaaata gcgatccata ctgttcctgc agattgacct tctaacgtgt ccggtccagc
    10621 cactccggtg aaagctacac tgtaatctgt tcttgctatc tcacgtgctt gaatggccat
    10681 ctgctccaca cattctttgc tgactgttcc atattctgct aatagctccg gatcaatctc
    10741 cagaaaacca gcttttgttt ggagcgagta ggtaacgaat ccgcctggaa atacttcaga
    10801 tacaccagca atgtcgccta atgcggcttg gaaggcgccg gctgtcaaac tttccgctgc
    10861 tgttacagtc tgcttgtttt ctttcagtag ttccactacg acttttgcta gcgaattatc
    10921 atcgccgtat ccataaaaat attcccctac tcgttcttgg attttttctt ctaatgctaa
    10981 aagagcttga ttccctgcct gaacatcatt tgttttcact gtcagtcgta atgtcacttc
    11041 atttggtttg gcatatggtg cgatcgttgg attgatctgt gtctcaatca gatcttttag
    11101 ctcagtgacc agtcgcgatt caccaatccc ataaaagcgc aaaactttag aaattagttt
    11161 ttcttcagaa ggaaaacgtt cctctagtaa aggacgtact tgttccacaa acattggttt
    11221 caactcatta ggcggaccgg gaagaaggat atacgcatgt gtatctgttt gataaaaagt
    11281 ccctaaagca agccctgtac gatttggtag aggaaccccg ccttcgataa tctgggactg
    11341 ttgcagattg tttttggtca tttttcgatg ggttgtttca aaatatgcca ataatttttt
    11401 gtatccttcc gtattttgta tgagagactt accgaggtga gccgcagttg cttcttttgt
    11461 caggtcatct tctgtcggtc ctaatccgcc acataagagt atgagttcac ttcttgtttc
    11521 agctagagca atcaactctt ctaaacgctg ctgattatct cctacaactg tttgataata
    11581 aacttcgata cctagatcag ctagctgttc tgacaagaaa gtggcattcg tattcacgac
    11641 ttgtcctagt aaaagttctg ttccgacagc tattatttct gctttcatta cttcccgctc
    11701 ccttatatag tatacgctgt atttttatat ttttttattt aaaaagaggg caaggagctg
    11761 tgacaaaaat cttgtcacag ctccttgttc caaataaaca gtgttctcct gccaaaacct
    11821 cggtcataag tcacaatata cataaaacat gaaaagctgt tttttgaaat tatcccttgt
    11881 gactcagagt tagacggcag gctcacaacc tctccactaa acgatactta cattgatcct
    11941 ttgaacacac cgctgttttt gatgaagtaa tccacacctg aatagatagt aaagatcaga
    12001 caagcataaa gcatgatttg atctactgga ataccgatca ggctgaatgg gatattattg
    12061 ataaacagca agataatagc aaccatttgt gtagccgtct tgacttttcc cggccaagct
    12121 gcagccatga cttctccatg ttcgacaagt agtaaacgca agcctgtgac ggctaattca
    12181 cgacagacaa tgatcgctac tacccaacca ggggcctttc cttggtcaac caacatgata
    12241 aaagcagtca taactagcat tttatctgcc aacgggtccg caaacttgcc aaaattagtg
    12301 acaaggcctc gcgcacgggc aatttttcca tctaaccagt ctgtgatact agctaaagca
    12361 aaaataactg cacctactag ctgtgtgaca gccagtgtgg tgtctcctac gttaaattgt
    12421 ccccaatcta aaggtaaacc tacaaccaaa atgaaaatcg ggatcataca aattctaagt
    12481 acagttaatt tatttggtaa attcacacta tcgactcctt tcttccctat tctacgtgac
    12541 tatcagcgaa gtttccaccg atattattta cttttccaaa tgaatttatt caaaggagta
    12601 tttttattga aattgatctt tttgaattaa cgaagaagga ccgcgacaca gatttgtcac
    12661 gatccttctt ttttatacct acaagtaaac agatgcgtaa actaatttat acgatcattc
    12721 tgcgtattga agagtaaggt tcacattttt tggactctga ttgttttccc ctggattaac
    12781 tggtacggat tctccattcg ctttgatcgt tgcattgctt gctgcaccaa tcgtgatggt
    12841 cgcatttgtt gctgtttccg gtaacacggt actctgtgtt tcatttgctg ccagtgtccc
    12901 ttgataaaca taggcattgt ctactagcac acctaaccat acacgattga cagcagtaaa
    12961 ttcaagggtt acaggcttgg tagcgtctgt gatatttact gcgatggcac tgccagaatc
    13021 ttctcctgta gctattgcca ttttcttttc ttcacttgat tttgttgttg attctactgt
    13081 agaagactct ttggtctgcg tgctttgctc tgaggaagag gactcctcga ctgatccttc
    13141 tactttaagc gaagaagctg tgtctccgat tattggatca gcttgacgat cttgccaagt
    13201 catatagccc acgacaacga caatcgccaa agcaaccaat cctaataaaa tgacaggaag
    13261 acttgtccaa aagcgacttg gatgcgattc ttcctgatgc attgcttttc tagaagtact
    13321 tactgtttca ggctgcggac gttcagggac cgatcctgtc aatttgcttt tcccatcaaa
    13381 aacatcgact aaatggtctc catcttctcc tacagcttca gcgtattgcc gaataaatgc
    13441 ccgcacgtaa aatttaccag gcaattggtc aaaatcatca ttgtcgattg cttccaaata
    13501 acgtttttga atttttgtaa tctgctgtaa ttcatctaaa gatatttttt tattcaggcg
    13561 tgcttgccgt aatttttctc cgatggtttc aaaagccact cgttcatctt ctccagtctg
    13621 tttgattctc cgaagcatct gctgttgcaa cgaaagacgt tatgtttggg tgctgtgaca
    13681 aaagtcatgt cacagctcct tttttccgaa taaacattgt tctcctgcca actcctcggt
    13741 cacaagtcac aatatccaat aaatataaga agctgttttt tgaacttgtt ccttgtgact
    13801 cggagttaaa cggcaggctc acgacctctt tcttaaacgg tgttcaatca gctgttcctc
    13861 ggtattagct caaaaaccgg caaaatataa ggagatattt ttccgtatgt ttctctaata
    13921 ctcaggtcaa aacgctgatt tcacaacctc ttccttaaac ggtgttcaaa agcttgcatc
    13981 tgcggtaata agtccaacca agttaaaaac attaagaagc tgtttttact tggctgttct
    14041 tattacttga tgcagggcag ctttttcaca acctcttggt acacggtgtt ccagaagtag
    14101 cttcttcggc aataagccga aatatcacaa aaatttgaga agcaattttc gtaaattcct
    14161 tcttatttct tgaagctgac cacttctgtc acaacctctt tgattacttc gtttgatttt
    14221 attttaatat aagttgatta aaagagagca tgatttgttt tattttctca ctaatttttc
    14281 caacacaact aagaatacca taaaagctga ttttggagga ggttcctact acaagtctaa
    14341 aacgaaattt aagacttttc ctcataaaat actggtttat tttggtctca tgtaaaactc
    14401 gctgaaggcc tcttcactta taaatgcttc cccagctgcc agtacgtctt tcatttggat
    14461 cgaatcgatg atttcaggaa gatcaaataa agttcgatcg ccaaataaac tttgcgtaaa
    14521 ttgattggca atatattcga tcgaatttaa tgattggaaa tattgtccaa gcattttctt
    14581 cttcagcaaa tcgagatttg tttctgacac ttccggatca tcagcgaatc caagaatgat
    14641 ttctttgact ttttctgctg ctttctctgg ttcgtccgta tctccgctga agtccgcaaa
    14701 gtgaaattca cgatccaagc tgaattcaaa accaaagctg tcatcgataa ttccttgatt
    14761 gtacatcgct agatagtttc gagaagtatt gcctaaaatc atttggaaca acagattgat
    14821 tgctgtttta tagcgtaaca gttctgttcc ttcttgcggc aatgtgtcta atcctttaat
    14881 accaagtaca aatttatctc gtgtaatggc tgcttccaat gcactttgtt tgatgatttc
    14941 ttttgtgttt tcagggaaat aacgtacaat ttcttgcttt ggagggaaat tttttgcttc
    15001 ttggttttcc cgaataagtt tcatcaattt ctcaggctcc atcttaccaa ctacaaataa
    15061 aaccatattg cttggctgat aaaacgtccg atagcacgta tacaagtctt gagctgtgat
    15121 tttgtcgata ctttcaaccg tgcccgcaat atcgatatgc aatgggtgtg taggatacaa
    15181 gttatttagt atgccaaaaa acatccgcca gttagggtcg tcttcataca tttggatttc
    15241 ctgaccgatg attccttttt ccttgtttac agtttcttct gtgaagtaag gtgcttgtac
    15301 aaaatcgatc agagtcgtca gattcttttc tacctgatcc gtcgtagaaa acagatagct
    15361 ggttttcgta aaacttgtga aagcattggc ggaagctcct tgtttcccaa ataattgaaa
    15421 gacatcacca tcttcttttt caaacaactt atgttctaaa aaatgggcaa ttccatctgg
    15481 aactttcacc ttttcttttt caccataggg aataaattcg ttatcgatcg aaccataatt
    15541 cgtactgaac aaaccatatg tcttgtgata gtcattttta ggcaacaaat acacggtcaa
    15601 accgtttggc agtacttcat gataaagtgt ttcattgatt tgctcatatt ctgttttatt
    15661 catttgcatt tcctcctgct aaaaagaaga ttgcctgcaa ttcgatttgc tcagctactt
    15721 gttgtatctc tttgatcgtt acgctctcca tacgtttcaa ccattcttca tcagataatt
    15781 ttgtttccgg cagccagctg tccaaaaaag aagcttcgat tgccgcttgc ggagagtcta
    15841 gagaaagcag atattggttt ctcagcattg cttttgtctg agacagttcc aactctgtta
    15901 tttcaccatt tcgcaaactt tctaattgct cgtgaatcag atgaagtact tgatttcggt
    15961 tcttttcatc gataccggtt tggacactca tgaatcctcg gaacgtatcc acgctactag
    16021 aggcataata agctagactt tctttctctc tgacattcat gaaaagttta gagtgaggaa
    16081 aaccgccaaa taaaccgtta aagaccatta gcgcaaaacg ttctggttca tcgtaataca
    16141 catttgtctg ataagctagg ttcagctttg cttgaacgat tggctcacgt acttgtcgtt
    16201 ccttcacgat attcacttga ggttgggtat aaaacagatc tggataaact gtttcccgcg
    16261 actcaaatgg taattgccca actgcttcag ctgctttgtc cggatcaaca tcgccgacaa
    16321 caaaaatgtc gatttgatca tttgccatca tttcttgata gtaggacgct agcgaactag
    16381 cagtgatctt gtccaaatct tcgactacgc ccaaccctgg gatcttttgt tccgggctgt
    16441 tttcaaaata aagttcttgc aaggcatagg aagcaaaagt ttgtttatct tccttcaaac
    16501 tttccaaata tagacgcaga ttgttctttt ccaagtcaaa cgtcaactga tcaaactgat
    16561 gatttttaat atttggataa aataaaacct cttttaaaaa atctaccgct tgagaaaaaa
    16621 ggtttggatc attgacatat tcaccattca caaaactgat ccctgcgttt agccaatgta
    16681 aatttccttt ttttctaact gataatccaa agcttgcgcc ataaagctct gcaagttgcg
    16741 cacttaactt tgtctgatcc gaataattca aactatttgt ttccaacatg ctgctgagaa
    16801 gtgaacgttt cgtgatgact tcttgttgca gacgcgcagt aaaacggata aaaatccgga
    16861 tcgttttgta ttgttttgtt ggtaagacat gaagattcac gccttctgcc aattgataca
    16921 ccatatttat atgctccttt tcacagttga ttgcccgctt actcttttaa tgggtcaacc
    16981 tgcctttttt acttattcct ctgcttgaaa tttataagtt caggcaattc gccgtttttt
    17041 ctaaatgatg ttacacttta tagtatagca tagatgtttt ttatcaaaag aaacagacgg
    17101 ctaaaagcgt atattgtttt tttatttttt atttttcgat atactaaaga caaataaaag
    17161 aagggagggt cattcatgat caaagaattc aaagagttca tcatgcgtgg agatgtcttg
    17221 gatcttgctg tcggcgttgt tattggtagt gcgtttacaa gcatcgtaaa ccaagtggtg
    17281 caaggcttga tcacaccact aataggttgg tttgtcgcaa tgatcacagg tacttctgat
    17341 ttagaaggag cactttcaat tcttgactgg tcgccgacaa aaggcgtcac atttgcgttt
    17401 ggtaacgtta ttagtgctat cattacattt ctgatcactg gttttgtctt attcttagtt
    17461 gtcaaagcgg caaaccgtgc gaagaattta cgctccaaag aagagcaagt ggaagtggaa
    17521 gaaactccta ctgcagaaga ttatcttagt gacatacgcg atcttcttgc caaacaagtg
    17581 gcagaagaca atacacatcc agtagagcaa acaaatcttt ccgatacttc taaataataa
    17641 agaatctaaa aaaaaagaaa gccgattttc agtgtcggct ttcttttttt agattcttaa
    17701 cttgctactt gttccttttt catgtcgata tgtggaatat catcttccaa atacacatct
    17761 gagattgcct tgaatccaaa cgactcataa aaagcttgta ggtattcttg tgcgccgatc
    17821 acgattggtt tttctggaaa atttttctgg atcatgttaa tcgtttcttg aacaatcttt
    17881 cttcctaaac catctttacg gttgtcctga tgaacaagga cacgtccaaa atggatactc
    17941 tccgcttctt catatactcg cgtgtaggca aggatcttcc catcagtatc ttgaaaaaac
    18001 gtatgccaag cttctccatc aatgtcatca acttcttgat aaggacactg ttgttccaca
    18061 acaaaaactg cgattcgttc tttgacgatc gcgtaaaact ctgctgttga caattcttca
    18121 aatttcttta cctgatattc cactaagcaa acgccccctg cttttttatt cttagcatac
    18181 cgattcgcac acaaaaaagc aaacgaacct tacgaatctc aggctcgttt gctttttaaa
    18241 ctgcttcagt taaaaattcg atctcaactg tccgagtaag tacatctgaa tagctgtaag
    18301 atactcgttc aaaagaattt tcttcaggat ccaaatcaac aacgaatact gaaggatagg
    18361 tttctgttaa aacgccgcgg cgttctgttt gacgctttct tcctgtctga gcaaccaaca
    18421 tgattgggct gccgatacga ccttctaaat ctttcttgat tgaagctaaa gttgttggca
    18481 tacagttcac ctctactatc aactatacca catattttta aaattttcaa gtttaccata
    18541 aaaaaaaaag aaatgcaata aaaataaata agaagctcat taagattttt ctaaattatt
    18601 cctcattata aaatttcaag tgttttttta ttttgagttt tactctcccg taagcgctca
    18661 ttacttgctt gtgatccatt gctaaacatt cagccatgtc tacaaaatca acgccttgca
    18721 tataataatg aaaaatttta gcttctattt ttgaaaactg gatttcgcaa ttttcgattg
    18781 cttcttttaa taaaagatgc tgaattgcta aaggttcttg caatgtgatc tctgctaaaa
    18841 agtctggtcc ttctgtctct attttttggt cgattgagac ttcatctttt tgatcacgac
    18901 gcttcagcgc gttctgcttc ctcaacagag aatagatttg attgcttata acgcttttat
    18961 aataacttcc aaagctaaca cctttgcttt tttcgtaagt tctcaatgcc tgatataaag
    19021 cgatccttgc ttcttgttcc caatcctcag aaacataatt tcttaaataa taggtgtttt
    19081 tcaaacgtag taaaagtgga aaatattttt gatacagggc agcaaatgct tcttcgtcac
    19141 cattacgtgc agcgcgcaat aactcctcag acataaaata attcctccat aaattcgttt
    19201 atggaggaat cttatcatca atgattcatc gaactgatta gaacgatatg ttatttattt
    19261 tttctcatta aatcgtccaa tttttgggac aattttttta actgctcttc attccatgga
    19321 gaattacgtc ggaaatcaat aaaatgcata tctttatttt cttgggctat tgttttttcc
    19381 gtttttttta tcgtcttata taattcataa gcagaagttc gtaatgctcc ttgggaaaag
    19441 atcatccact gttctgctaa atcgcttgtc gccactgtca cttgtgtcaa acggtcattc
    19501 agctgaccag cgattctctc gatataactg tcagccgttt cgtcttcttt cgtaaaaaca
    19561 acatccaact ggtatttctt ataattttgt tggatgcccg gaactagttg ggcatcgaag
    19621 acaacgatga cttccattcc ttcatatttc gcataatttg ataaacgatg taaaagagct
    19681 tctcttgcat ccgccatttt gtcctgcttt ttaagctgga caagttccgg ccacgaacca
    19741 atcatgttgt agccgtctac aatcaacagc tgttttttca tggtctactc cccctgacgt
    19801 tgggaaaaag cctgatacat aagcaaacca gcagcaacac tggcatttaa gctctggacg
    19861 tgcccggtca tcgggatcgt cagcaattca tccatttctt ttttcaatcc tggactcatt
    19921 cctttgcctt cattaccaat gactaaagca attgatcctt tggcattcca tttttggaag
    19981 tttgtgccgg tcatatctgt gccaaatacc caaaatcctg tttctttgag ctgttgcaca
    20041 gcttgtacca aattcgtcac gcgtgcaaca gggacatgtt cgactgcccc agtggaagtc
    20101 tttgttacga taggtgtgat tccaactgct cggtgttttg gtatgatcac accatcgacg
    20161 ccacttgcgt ctgctgtccg taaaatcgac ccaaaattgt gtggatcttc gatgctatcc
    20221 aaaatcaaaa agaaggggtc ttcttttttc tcttttgttc gttcaatcag ttggtcaagg
    20281 gataaatatt cataaggtgt aatcgcaaga acgactcctt gatgcacgcc gtgatcgctc
    20341 aaagtttcta atttttgttt aggaacccat ttgactggca cagcttgttc acgtgcgatt
    20401 tctttcagtt cttctacttt ttctcctcgt gaatcttctt gaagaaagag tttattccct
    20461 cgcccttgtt gtagcgcttc tactgcggca tggtgcccaa agacgaaatt atcttctaat
    20521 gcttcttcat tttgatcaga aactgactga tttcgtccag tttgcttttt atttgaatat
    20581 ctattttgtt ttttgtcttc aaatccacgt ttcgattgat gtttgtttgt tctttcttta
    20641 cccatttttt tcaccaactt tctgaataca ccagcggatc aattcttcca tccgctcggt
    20701 ttgttttgtc aaatgcagat agcccatgag cgcttcgaac cctgttgcga cccgatacgt
    20761 cgtaacatca gcattttttg cgctcgtatg gcttttagca tttcttcctc tgcgataata
    20821 aagttcttct tcttctgtca aaagatgttc ttccagcatt tgctgaatca aaaaggcttg
    20881 tgcattcgca gaaacataat tcgttgccgc acgatgcagc ttattgggtt ttgtttctcc
    20941 ttgatcaatc aggtggtcac gaacatagat ttcgtaaatc gcatctccaa cataagccaa
    21001 ggctaaaccg ttcaattgtg tgtaatctct catgcttctc ttctccatcg ggttccttgc
    21061 ggtgtatctt ctaataagat ccctttttct ttcaacaaat cacgaatttc gtcgcttcgg
    21121 acaaaatcac gattgcttct tgcttgattt cgttcttcga tcaattgttc aatctcttca
    21181 tctaataact cttctgctgt aaataaaata ccgaagatcg aaagccattg cgagaataaa
    21241 gcttctcctt gtgccaagac agcggcagaa actttcggct ggtctagata agtgttcagc
    21301 cacttagcaa gctcatagac cactgtgatc ccatttgctg cattaaaatc atcgtccatt
    21361 tcttcgatga atcgctgttc taaattttct aacaaagcga ttttatccgc atcatctgcc
    21421 aaattctctg aggcagtttc ttggcggaat ttcaaattgt caaacgtatt cttcagtcgt
    21481 tgtaaattgg tggctgcttc tttcatcgtc gtttcactgt agcggatcgg acgacggtat
    21541 tgtgtggtcg ccatgaagaa acgcaagact tgtggatcaa cgtttttgat catttcatgt
    21601 acagtgatga aattccctag agatttactc attttttcat cgtcttcgcc aatcgttaca
    21661 tagccattgt gcatccagta attagcgaat tttttccctg ttttcgcttc actttgtgcg
    21721 atttcatttt catgatgagg aaattctaaa tcttgcccgc caccatggat atcgatcgtt
    21781 tctcctaaat gttttgttgc cataacggaa cattcaatat gccaaccggg acgtcctttg
    21841 ccccatggtg aatcccaaga aatctcgcca ggttttgctt gtttccataa agcgaaatct
    21901 aatgggtctt ctttttgctg ctgttcttcc cctgtacgct gacttgctcc gacttccagt
    21961 tcatcgatgg attggtcact taatttccca taattttcaa atttacgggt acggtaatac
    22021 acatcacctg aagattcata agcatagcct ttttcaacca acacttgaat aaatgctaaa
    22081 atatctggca tatgatccat gactcttgga tgcagtgtag caggttcgac attcaacgct
    22141 ttcgtatctt cttcaaatgc tcggatgaaa cgatcagcaa cttctggtgc tgtaatacct
    22201 aattcgtttg ctgtacggat gattttatcg tctacatccg tgaaattcga tacgaaatcg
    22261 actttatagc ctcgatattc aaaatagcgt cgaattgtat cgaaagcaat ggtgctacga
    22321 gcatttccga tatggatata attatacaca gtcggtccgc agacatacat accgacatgt
    22381 cccttttgta tagggatgaa ttcttctttt tttctggtca atgtattgta gatttttatc
    22441 attctgatct cctcactttt tctcctttga ttcgaacgac ttttgctggt actcctaccg
    22501 ctgtagcgtc gtctggaaca tctttcaaca caacggcacc tgcaccgatt ttggcgtttt
    22561 ttccgatcgt taccggtcct aatatctgcg cattcgcaga gagcatggct ccttgtttaa
    22621 cagttgggtg tcttttaccc gtttcttttc ctgtgccgcc taatgttacg ccatggaata
    22681 aaatcacatc gtcctcgatt tctgcggttt cgccaatgac gattcccatt ccatggtcga
    22741 tgaacacgcc agttccaatc gttgctcctg gatggatctc aacaccagtt aggaaacgcc
    22801 agaattgagc attgatcttc gccaataaaa agagccggtg ctgatataaa aaatgagaga
    22861 aacgatgcca aaacaaggca tgaaaaccag ggtatgtcaa aacgatctca gctgtcgaac
    22921 gtgcagccgg atcgtttctc tttgctgcat caatcgctcg ggtaaaccaa ctcatgactt
    22981 tccactcctt tcgtaagaat tttatgtagg aaatcatctt cttataaaga aaagaagcgc
    23041 cttttaaata agtttcctta tcaaaagacg ctttccgcgt ggttccactt ttcttcataa
    23101 tcaatgatta tctctgacag ctaacgcctg ttatacgttc ttggctactt tgtttcctat
    23161 aaaacagttt ttcctgtttt ctgtgtttcg ccaagaccac tcccagaggc atttcggcaa
    23221 ccattcattg ataccttgca ccacccggta tctctcttgt aatgatcagc tgcttacttt
    23281 tttctgttca tcgtgtttag ttcagtactt gatttaaatg tgctaatgat ttttcacgac
    23341 caagcaattc gatcgtttca ggcaattctg gaccatgcat ttgtcctgat acagctacac
    23401 ggattggcat gaaaagattt tttcctttta cgccagtttc tttttgaaca gctttgatgg
    23461 cagctttgac agttggagca tctactactt ccatttgttc tagctgtgct ttgaatgcgt
    23521 ggagaacagt aggaactgtt tcaccagcaa gtacttcttt tgctgcttca ttcaatacag
    23581 gatgttcatt gaaaaatagc gtagacagat caacgatttc tgcagcatag ctcatttgag
    23641 gttgatacag gctaacgatc ttcttcaccc attcgattgt cttttcatct gggttttctg
    23701 ctacacgtcc atcagcgatt agataaggca gacacatgtc agtaaatgca tctagatcca
    23761 ttgctttgat gtaatggttg tttacccatt gcagtttttt cgcatcgaaa gctgcgggtg
    23821 atttacttag gcgttttgga tcgaacattt tgatcaattc ttggtgttcg aagatttcgt
    23881 cttcgcccac aggtgaccag cccaataatg caataaagtt gaacattgca tctggtagat
    23941 agcctaattc gcgatattgt tcgataaatt gcaagattga ttcgtcacgt ttgcttagtt
    24001 ttttacctgt ttcactgttg atgatcaatg tcatatgtcc gaaggttggt ggtgtccagc
    24061 caaatgcttc atagatcatc agttgttttg gtgtattggc aatatggtca tctccacgta
    24121 aaacgtgagt gattttcatc aaatgatcat cgaccgcaac agcaaaatta taagttggca
    24181 taccatcgcg tttttggatg acaaagtcgc caccgatatt atcggattcg aacgaaatag
    24241 cgcctttcac catatctgta aatgcgtatt ctgtattgcg aggcacacgg aaacgtacaa
    24301 cagattctag accttgtgct tctttttctg cttgttcttc aggtgtcaag ttcgcacatg
    24361 ttcctgcgta atgaggcatt tctcctcttg cgcgttgtgc ttcccgttct gcttctaatt
    24421 cttcttctgt gcaatagcat ttgtatgctc gattgctagc aagcagctga tcaatcagcg
    24481 gttgatagat ttctttacgt tcagattgac ggtaagggcc gtattctcca ggattttcag
    24541 gagattcatc ccaattcatt cccaaccaag caaggttttc caactggctt ttttcgccat
    24601 cttcgatatt acgtttttgg tcagtatcct caatacgaat aataaagtct ccatcattgt
    24661 ggcgggcaaa caaataattg aataatgcag ttcttgcatt tccaatatgc aggtgcccag
    24721 ttggacttgg tgcatagcgt acgcgtactt tcgtcatttt cttcctcttc tttcaaacta
    24781 gactttgact aacagcaaaa attccgtcag ttacggcttc tcaaatatca tttatttaaa
    24841 gccactcagc acaaatttta ccttttctaa ggtaaatagt aaagccaact ggttgaattt
    24901 ccttaccagt tggctgaatt tcttttttat tttgattgtt tagtcttttt ctctgtctct
    24961 tttttattgt cgattcctcg tcctgcatgt gcaggtttag caaagatcat gcgtccagca
    25021 gctgtttgaa gggcacttgt tacaacgact tcgatacgtt cattcatgta atgctgtcca
    25081 tcttctacga caaccatcgt gccgtcatca aggtaggcta ctccttgttg gcgttctgtc
    25141 ccggctttta ctactagaac attcatcgtt tcaccaggga tgacgactgg ttttacggca
    25201 tttgccaacg cattgatatt aagaacaggt acattttgga attcagagac tttattcaag
    25261 ttatagtcat tggttacaat gacaccatct aaaagcttag ccaatttgat caatttgctg
    25321 tctacttcac tgatatcttc aaagtctcca tcgtacattt cgacagatat accctcttct
    25381 ttttgcaagg catttaggat atctaatccg cgacgaccac gcacacgttt caaactatcc
    25441 cctgagtccg cgatgtattg tagttcgtaa agaacaaaat tagggatcat caaggttcct
    25501 tctaagaatc cagtctttgt aatatcataa atccgaccgt caatgatcac gcttgtatcc
    25561 aagattttat atttatggaa attttctcct gattttcgtt ctaacagttg actctcaact
    25621 tgactagctg tttcttgctc gatttttttg cttcgtgaac caaaaacttt cttccattct
    25681 tcgattcgtg tcgttcccat tctcaatccc agatagccta agatgatcat cagtaaaatc
    25741 ggcaatacac tattgacgaa tggaatttcc atattgtaaa gaggggtcga gatcacaaca
    25801 cctaaagtca atccgatgat cgcaccgatc gcaccgaaga gaagataagt caagctgacc
    25861 tcacttagtt tttgttctac ttgtttgata atagctgaga tatgtttggc caacagcaaa
    25921 gataataaaa agaaaataag tgcgcctaac aggctgttgg taaatgtatt attcagccat
    25981 ccgtttgact gttgaccgat tgcctgccaa gctgagggta aaaatgaaat acccaagctt
    26041 gcgccgacaa tgatcatcag caaagtaatg acacgttttt gcatcgtttt tcctccgttt
    26101 cgacaatcat ttattagtta gcggaacaca cgcttcaacg tttcagccaa agttgctact
    26161 ccaactatct cgattccttt tggcgcttcc cagttaccta agttgttttt aggtaggtat
    26221 acttttgtga agccgagttt ttggacttct ttgactcgtt gttcaatatg actgacacgt
    26281 ctgatttctc cagtcagtcc aatttcaccg atgaaacatt ccgttggctg tgttcctttt
    26341 tctttatagc ttgaagcaat actgacagcg atagctaaat caatcgctgg ttcatttaat
    26401 tttaccccac ctgcagcttt taagtaagca tcttgattct gaagaagcaa cccagctctt
    26461 ttttccagca ctgccatgat cagagatacc cgattgaaat caagacctgt tgttgttcgt
    26521 ttggcatttc cgaacatagt aggtgtgact aatgcttgga tctctaccaa aattggacgt
    26581 gtcccttcca ttgccacaac gatagcagag ccagttgcac cgtctaggcg ttcttctaag
    26641 aaaatctgtg aaggattagc gacttcttcc aatccatgct catgcatttc aaaaatccca
    26701 atctcattcg ttgagccaaa tcggtttttt actgctcgca aaatccgaaa actatgatgt
    26761 tgttcacctt caaaatataa caccgtatcc accatatgtt ctaacattct tgggccggcg
    26821 atcgagcctt ctttggtaac atgaccgacg ataaaaatgg ctatcccatt tgttttagcg
    26881 gttttgagta gttctgcagt ggtttctctt acttggctga cacttcctgc cacacttgtg
    26941 atgtctggct gcgtcatcgt ttggatggaa tcaatgataa cataatcagg ggagatatgt
    27001 tcgatggcac gactgatttc gttcatatct gtttcagcgt atagataaaa ttctgtgttg
    27061 atacttccga ggcgttccgc gcgtaatttt atctgctctg cgctttcttc gccggataca
    27121 tataagactt ttccgcctat ggctgctaat tgctgagaaa cttgtaacag caaagtggat
    27181 ttcccgatac ctggatcgcc accaatcaag accatagacc ctggcaccac acctccgcct
    27241 aacacacggt tcagttcttc taattttgtt ttgatccgag gttctttttt agggacgacg
    27301 tctgcgattt ttgttggttt tgctttttct cctgtcaaac ttgtccgagt tctgcggtcc
    27361 gttgtatctt gttcgatttc ttcgaccatc gtattccatt ttccgcaatt tgggcagcgt
    27421 cccaagaact taggcgacac atacccgcaa ttttggcaaa caaattgtac ttttgatttt
    27481 tttgccattg taccgcctcc ttttatccta atcatgagac attaaatcaa gcttaagtat
    27541 ctttaacgtt ttatttactg gaagaaccaa atccgccttg acgttgagca gtcgtttgat
    27601 cttgatctgc ttttaaaaac ggcaaaaaga tcccttggcc gatacgctcg ccttttttga
    27661 tcactacatc cttgaaacca aagttggtga attggaacat gatatggcct tcatttgcct
    27721 cattattgta ataatcactg tcaatcacac ctactccgtt tgtcaacact aaaaaccgct
    27781 tcaacggatt gcttgaacgg ttagcaagct gcaaaaattc atcttctccc atgtaagctt
    27841 tgattccagt aggcacgagg gttggtttca attgtttttc attttctgat acatcttttt
    27901 tcaatccatc tgcgactgtt tccaacccct ttttccagcc tgctttccag atacttggaa
    27961 tcacgacctc ttctgctgct tcaaaatcat agccagcagc atgatgtgta gcgcgctgcg
    28021 gtagattgat attttgatct ttatatttag aaataacttc aaatcctctt tttctcaccg
    28081 gttttcctcc ctaaataaac tactcctatt ctaacatatc cgaggctgta aaagactttc
    28141 tttacaaatt cttcaagaaa ttctgctatc ttaaagtaaa ccattaattc gttctttttc
    28201 caaacgactg caagttgtac aaaaaaacta ttttttacat gactttactc gtcaaaacaa
    28261 gtagaataga acgaaattaa tgagtggata tttgattgag gtgtcacatg cgtaaagaaa
    28321 ttaaaaatgc aaaacgaatt attgtcaaag taggaacaag tacattgatt tatcccaatg
    28381 gaaatatcaa tcttgcggca atcgatcagt tagcttttgt cctttctgat cttagaaatc
    28441 ggggaaagga agtgatctta gtctcatcag gagctgtagg cgttggtatg cataaattac
    28501 atttgtcaga acgtccgact ttgattcctg aacagcaagc tgcggcggct gttggtcaag
    28561 ccgaactgat gaatatctat aatcaaagat tcagtgtata taatcaacag acggcacaga
    28621 tcctcgtgac aagagacgtg gtagattatc ctgaaagtcg taaaaatgtg aggaatacat
    28681 tggaacagct tttgattatg gacacgatcc ctatcgtgaa cgaaaacgat actgtagcag
    28741 tggatgaatt agatcatctg acaaaatttg gcgataatga tcagctgtca gccatcatgg
    28801 caaaacttgt cgcggcagac cttttgatcg tcctttctga tatcgacggg ttttattcag
    28861 ataatccgac tacaaatccc gaggctcaga tgtatcgcta tatcgataaa atcgatgaaa
    28921 agattctggc acaagcaggt ggtgctggta gcacatttgg aacaggcggt atgcagagta
    28981 aattaaaagc cgcttctagg atatttgaag caaacagcgc aatgatttta gcaaatggaa
    29041 aaaatccggc aattattttt gatattcttg caggaaaaga agtaggtact ttatttaagg
    29101 aggacttgaa atgactgact tgatgcaatt aggacaacag gcaaaagata gcgcgcaaca
    29161 actagcactt atgcctacaa accgcaaaaa tgagctactt gttcaaatgg cacaaaccat
    29221 caaaaagaat caacaagcga tcattgaagc aaatcataaa gacttagaaa aagcagtcga
    29281 aaacaatatc agtgaaacga tgcaagaccg cttgcagctg acacctgagc gaatcaatgc
    29341 catggctaca gagatcgaaa aaattgcttc gttagacgat cctattggaa aagtagatga
    29401 gatgtggacg aacaccgacg gattgagaat cggcaaaaag cgcgtgccac taggcgtgat
    29461 cggcatcatt tacgaatctc gtccaaatgt cacgacagat gcagctagtc tagcattcaa
    29521 atcagggaat gccgttatcc ttcgcggtgg caaagaagca ttcttctcga atcaattatt
    29581 agtccaatta ttgcagcaag tactggtcag tgctggcgaa tctccttatg ccatccagtt
    29641 tgtcgatgat acttctcatg aaactgcagg acagttgatg cgattgacag aatacttaga
    29701 tgtgctgatt cctcgaggcg gagcaaaatt aatccaacga gtcaaagaac aggcaacggt
    29761 acctatcatt gaaacaggta caggaaataa tcacgtatat atcgataaag aagcacagtt
    29821 atctatggca gtgaacatca tcgtcaatgc aaaagcttcc cgtccatctg tctgtaacgc
    29881 ggcagaaaca ttgctgatcc attctgagat cgcacctttt ttcttgccag ctattgaaaa
    29941 agaactggta gaacacgggg tctctttacg tgcggatgct cgtgcgttag aatatctaga
    30001 gacggctgct ttagcagaag acacagattg ggatactgaa tatctagatt atatccttgc
    30061 tgtcaaagtg gtcgattctt tggatgaagc aattgcacat atcaatcggc ataatactaa
    30121 acattcagaa acgatcgtga cagatagtta tgcggctagt caacgtttct taaatgaagt
    30181 ggacgcggcg gctgtctatg tcaatgcctc cactcgtttt acagacggat cggtatttgg
    30241 ttttggtgca gagattggta tctctactca aaagttgcat gcccgtggtc caatggggtt
    30301 aaatgaatta acttccacaa aatatatcat ttatggcgat ggacaaatcc gcgaataatg
    30361 tttcgtgtga aacattattc ccgccttttg ccttttttct gtaaaggttc tatccaacta
    30421 gaaaggtgtc aatatgtttg aaattttaat gatttttttt atttacctta tttctctgaa
    30481 tatcgctgct tttctgggag tcggtatttt atcccttttc tttcaattca aaaaacgaag
    30541 catagggagt caacgggaaa aatggtcgca gtattttgac aaaatcgggc aaaaagggct
    30601 ggttacacgc ttgcacatta gttacatggt tgcattatgc ttattagcga caatcaatta
    30661 ctattctttt ttcgatcatt caatcgctta tacgattacg ctactgattg ctgggatttt
    30721 tcacttatct tataaatatc agctgaataa aaaccacttg aatcggactt tccgctaaaa
    30781 aatatccgta cgataagtag agtgccttga agatcggcaa aaaactgccg gactttttag
    30841 aggctgtgac aaagattttg tcacagcctc tttttccata taaacggcgt tcgcctacca
    30901 gctgcctggt aaatctgttt cctcttcgtt aagaagcgaa aatccgtcaa atgtttcgcc
    30961 atcataataa cgaagcagct catccatgga caagttcatc gttttttctt tttcttcttc
    31021 cgctgattca gccagtagct gttcccgata cacctgatac ataccagcta ttttttgctg
    31081 catcgctttt ctagtttcac cttccgcatt gattattggc aattctttga tatagccacg
    31141 ataaatcaac cgatcatctg aaaattcaat gggtaattca acgagtgtca acccgacttc
    31201 ctgaaaatat tcttccagca tctattatcc ccccattctt tttcttaaat gaattttagc
    31261 ttacctactg aaaattttcc agtttcatta ttttgagaaa aaaagggaat gtgacaaaaa
    31321 tcgtgtcaca tccccttttt ccgaataaat ggtgttcttc tgccaactcc tcaatcataa
    31381 gtcacaatat tcaacaaaca tgagaaactg ttttttgaaa ttgttccttg tgattcagag
    31441 ttaaacgaca gtctcacaac ctcttggtaa acggcgttca atcagctgac cctcggtatt
    31501 agctcaaaaa acggtaaaat atgagaagct atttttccgt tttgtttctc taatacttag
    31561 gataaaacgc tgattttaca acctctgtta aacggtgttc aatcagctgt ccttcggtat
    31621 tagctcaaaa aacggcaaaa tatgaggaac tatttttccg tttgtttctc taatactcag
    31681 gacaaaacgc tgattttaca acctcctgat aaacggtgtt caaaagcttg catctgcggt
    31741 aataagccca accaaacgaa aaatatgaga agctattttt gtttggctgt tcttatgact
    31801 tgatgcagga cagctttttc acaacctctg atttcttatt cttcttcatt tgtagcaaaa
    31861 taattacggt gataaatgat acaacataca ctgataagaa caatggcacc tgcaaaataa
    31921 aacgcatagc tttcaccaat atgttcacca atcacacctg caataaatgg tgctaaagct
    31981 ccaccgaaaa agcggacaaa actatagctg gacgaagcaa cgtttcgttg cagacctggt
    32041 atttccatag caatcgtcgt caacagtgta ttgataattc cttggaaaaa tcctgctaac
    32101 acaatgctga tcggtacaag gactggcata gaagttccag ctcctaataa cagcaaacaa
    32161 ataatgaata agacaaatgc tgctaaaatt gtccgataag tcgtaaactt ccgctcaaga
    32221 gccggtgcaa cgaaaatcga agcaatagcc agaagcacac cccagccaaa aaatacaaaa
    32281 ccgacttcta attcagaata gccaatcaat aaaaatggcg catacgcaag taaagtaaag
    32341 aatccgaaat tatataacaa tgcgattagt ccaactgaac gaagtttatg attgcttaac
    32401 gctttgacac cttcccaaag gcctactttt ctctttggtt tctcagtttc aggtaaaagt
    32461 accgctaggg ctataaaacc gcaaaacatc aaaaatgcta ccccaaaaaa tggataacgc
    32521 catgaaccac tgcccaagac cccaccgact agagggccaa cagccattcc gcagccaagt
    32581 gctgcttcat acatcatgat tgctttttct gtgttaccaa ctaaaatact gacgatcgtt
    32641 gctaaagcag tagagacaaa taaagcattc ccgacacccc agccggcacg taacccaatc
    32701 agcattccga tattcccaga taatccgcca agaccagcga atacaacgat gataaacaat
    32761 cctgtcatca atgttttttt caaaccgatc cgactagata agaaacctgt aaatagcatc
    32821 accacaccag tgactaacat gtaactagta aataacaggg tcgtttcagc tggcgtagcg
    32881 tttaactgtg cagcaatcgt ttttaagatt gggtctacta gacccactcc cataaaagcg
    32941 atcatacagg tagctgctac aactagcgct gaacttttcc cctttgtttc ttcatttgtt
    33001 tgttcttgta tatctatttg attctccaat atttccatcc ctttctctct attagcatca
    33061 tacatgattt attttacatg tcaatatgca catgtaaaac gtggaaactt tactgtgata
    33121 taataaagaa aataagacga gaggtaggat ttttatgaat actttagcct atatattatt
    33181 atgcgtgctt ttatccaaac cacgttctgg gtatgaattg aagcagctga tcacaatttt
    33241 ttgggaagcc catcacagtc agatctatac aacgcttgcc aagttagacc agcagggtta
    33301 tgtgaccgtt ttggacaaag acgagcactc tcaaaaaaag atctaccact tgactgatga
    33361 aggacgtgct cttgtagagg aatggatcaa agaggaaact cccgctccta cgcaaaaaga
    33421 tgaatttcta gcaaaaatct atgctttttc gacgctcgat aaaacaacag ctactggact
    33481 gctattcact agagaacaac agctgaacaa aatcatgcaa aaaaatcaaa aaaaattgaa
    33541 tgacctctcg ctagacaaga aacaagaatt tggacgttat gttgtgatcc agcgcagaat
    33601 tatgctttgt cagcaggaac tccaatggtg tcagcaagta agagaacaaa tgcagcagtt
    33661 ttttgagtaa gggataaaca gaacctttct agaaatatct attgaagagc aaattatctt
    33721 caataaaaga gcaatacagt tggaaaagaa aaagcgatcc gaacgacagg ccaaaaccta
    33781 tcgttcggat cactattttt ctagaactgc attcaagaga ctatgacaaa aagcatgcca
    33841 aggccttttt tcaaataaca gtgttccatc attcattact ttgaattgct tttagcacac
    33901 ttcttgaaag cgggccaacg atgatcaact gcaacggtaa agcggcaatc acattcattt
    33961 tccaagcatt catataagca gagaataatt gatcaggtat tccttgttcc atgatcacac
    34021 cgaataaaga catacatgtc accattccta aaaccatcaa gcatgaaata gttaagatca
    34081 tttggacctt gctttctgga tttactggta gtttaaaggc aatttttttc gccagcacac
    34141 cgatgatgaa gacatccaaa atgaaagcca ccacgaatcc aggcactaga ccaatcaaca
    34201 aacttgataa atgaagatct tgatgcagcc ataaattata cgcgctcatc cccaagacca
    34261 tcaaaaaaca catcattgtt gtaaaaatta ttccttcttt tttatttgta ggcaatctgt
    34321 accactcctt taaatttgtc acgaaaatca tcgtagcaca tttaaaaatt tctcgtaagt
    34381 acttttttta ttttctgccc ttttatttca taaacttcat cagccgtacg ctcgacaaag
    34441 taagaatcat gagaagtaaa caaaatcgtt cccggatata tttgcattag cttctctaaa
    34501 gcttcgatcg ttgccatatc aataaaatta gtcggttcat ctaacagcaa cacattactt
    34561 ggcttagtaa aaagcaaagc aatcgtcaga cgggtcgctt ctcccccact taatgtacat
    34621 aacggtttcg tcaaagtttc ctgagcaaac cctaagtgat tcagcaaact acggacaagc
    34681 gattctgaaa actctgtttg tctcatgaca aaacgaatga tggattcttc agatgtcatt
    34741 tgataaccct tttgctgata tacttgaaag cttactttcg gagagagaag gattccttct
    34801 ccttgtttgc ggatatgttc taataaggtt gtcttacctg aaccattttc accgacaatc
    34861 gcgatttttt tacctaaaga aaactgaaaa tcaccattta ccagtaatgt tctcccactt
    34921 ctttccaatt ggacattttg tcccatgatg ggatagcggc tgtggagttc aagagatttt
    34981 ggtactggaa aacggatttg tttcatctgc tgcggcttgg ttgtttctcc tattttttgg
    35041 agtcttctct ctaatgtttt cgcattcttt tggatggctt tttgtacact gtctttttgt
    35101 ttagaggaag acaaccgatc cagtctgatt tgttgctttt tctttttact ggacactttt
    35161 tccaactttt ccgcttgttt tttcttttct tggatcgatt tttccaatcg ttttttctct
    35221 ttcatgatcg actctgcttc tcgtagctga gtctttttct ccaattcctt ttgagtgaga
    35281 taggcactat aattccccga aaactctcgg atttctccgt cttttacttc ccagattttc
    35341 gatgctagct catctagaaa atagcgatca tgactaacaa aaaccagcgt accataataa
    35401 taacgaagct cttcaatcag tcgatcaata ctttttctat ctagatgggt tgttggctcg
    35461 tctagaagca tcccttgttc ataagaagaa agaagacgta tcaactcgat ctttcgttct
    35521 tcccctccgc tccatttccg ctctgaagtt tcttccagtc ttaactggaa atgactggct
    35581 aattccaaat ttaggtcctt tgcttcagcg agataggtta attgaggcag gtagttgaag
    35641 gtgatctctg tttggatttt cccatgatcg ggaaaaagtt caccggctat tagtttgagc
    35701 aaagtcgatt taccgacacc attttctcct atgatcccta tacgatcttg ctgatagaca
    35761 cgaagagaat caatagatag ttctagctgg ttgccaaagc tgacttgtag atttgttata
    35821 tttactgcta aattttccat atgttttaca ctcctatttc atgtaaaaca acaattatcg
    35881 atactaaaaa agagcagaaa aatctgtttg caaaaaaact gcatcacatc ttttcctact
    35941 ctgaacgaaa taactatgac taaatcacca aatctgtcat agctttcacc tattctactg
    36001 tcgttcgaaa aaatggacat actccatcca tgaaattgtt gtttatacat gtcttttgaa
    36061 tttatttagt tgttattcaa aagttcgaaa cgtaatttca tcgatgcagt catttttccg
    36121 acctcctttt ttattaggtt accacacacg attcaaaaaa agcaatagct actgaatagt
    36181 aactgaaaac cttttattcg aacatagtga tcttttctgg tgctaaacga acagactcgt
    36241 aaccttcttc taccgctttt cctattgata aaataataac tggaacataa cggtctttat
    36301 ccaaaccaaa tgcctctgcc aactgatctg cttcgaatcc accaattgga tttgtttcat
    36361 agccatgatc acgcgcaact aacatgaatt gcatagccgc taagctagaa tcgactttga
    36421 ctacatcgtt catttcttct cttgaaaaac tcttgtaata aggaatgatc gcagctaatt
    36481 gttgatctct gacttctttt ggcatcttcc ctgattcgta agcttggttg tagatttctt
    36541 cgccatattc gtaacattgc atatcgccaa aaatcatgac cattgcagaa gaagtatcat
    36601 tttgtctcgt attgaaacgg atcaaaggac gcaagatttc ttttgcttcc tcgctttcag
    36661 cgaccacaaa gcgccatggt ttcatattga cagaagaagg tgcagtcgtc gcttctttga
    36721 tcatttccag catttcttcg tgtgggattt tgtatgtttc atcatatacg cgaacggatc
    36781 ttctaccagt cataattgtt gaaaaatcat ttttttgttc cattattatt aacctctttc
    36841 gtttctgttg ttatattgca aagttgggta ttcaatttat ctaaaagtgt tagaaattgt
    36901 ttcatttcta catcgctgaa agccggggat agcacggaac ctgtatcttt atggtccatt
    36961 tcacaatgct tgattttctt ctgggctttt tctgttagcg ctacatacac ttcccggttg
    37021 ttctgaggat ttctctgacg cgaaacatag ccatttgctt ccaatacctt cagatgacga
    37081 gtaactgctc cttgatcaat ctgcaacttt tcttgcaaac tgctttgtag caatggattt
    37141 tcttctttca gtctcatcat gatctcgtat ctagtaagac ttataccgat attttcttca
    37201 aagatctgga cgattttttg atcggctaat ttgatttgat ataaaaggtg actaatctct
    37261 tctaaagaca taatttcctc ctttctaata attgactcgt caacgattga cttgtcaata
    37321 atagaataaa aaaagaaaaa agtcaaagaa tcagctgtgt aataaaaaaa acgctctcaa
    37381 tagaaaagta tagagaaaag aaattttttg tagaatgaac ggaaaaaatg ataaagaaag
    37441 cacaacgcta aaaccaagat tattgattct tatcaaacta tagaagatag ctcttctgca
    37501 gtagataatt ttacaccgcc aaaaatttcc gaaatcgcct tttgcaagaa tcccgtcgaa
    37561 tattagaaat tggatcagta tgcttaaaca gtaataaaaa agttatgaca agatgttgtc
    37621 acaacctttt ttgagtaaac ggtgttcaaa aacttgcatc tgcggtaata agttcaacca
    37681 aacaaaaaac atgaaaatct gtttttgttt ggctgttctt actacttgat gcagggcagc
    37741 tttttcacta cctcttttta ataaccataa aatcctattc cttcatatct ccagcccatc
    37801 ggtgttagcc aatctctttc cttagcactc aaggtatagt gatgggtacc agttttttca
    37861 tttggattaa atagtcgata aattggcttg gaaccaccag aataaaaact tagaccttca
    37921 taacgccaac cgatttttgt taaagagttg atctcagatt gtgccattgt gtaatgatgg
    37981 tctccactgt aaggattgta taaacggtag acaggttgtc cgctgtttgg tgcttgccaa
    38041 gcaatccctt catattccca gcctacagtt ggcagataat ttttttctcc ggcattcaat
    38101 gtatataaat gttctcctgt atgacggtta tacagacgat acatcggaga agttgaagtt
    38161 ggtgctgtat catactgtgt cagattatag gtttcgatta cattgttcaa tgtagaacca
    38221 taattaggcg cagtggcata acgacctgtt agccaagccg tagcatcttt gtaagagttc
    38281 gtattgcttt tccatgcacc ggaatagtag taaacgcctg gctgaaaaga agtcgtcttc
    38341 aataccgtta cgttatcttg caatgattct ttgtacgaag gatattttct aaaagcggca
    38401 ttcaccgtaa tccattgccc attcacatat tccgaagtag gcatcgtaac agattggcca
    38461 ttatagcttc ctttaatccc aaatagattg taattagggg cagaagccaa agtactgttc
    38521 ccccagccac tttcaacgat ggcttgagca atcatgactg atgcatataa gtcattggca
    38581 cttgccaaac tttgtgcagt tgaggcaata ctatcaatga acgcttgttg agaattagca
    38641 gatgagcgag cataaaaagc atagccagta tagttttcga attcagacaa atctgcttga
    38701 tgggtatgtg tcaatgtttg cccatctgtc aattggttcg tgatttcttc tgcattctct
    38761 ggattgttct gagcttctgc tgatgtactg tcttcggttt gtgtttgttc tgttgtttca
    38821 gttgttggtt cttcggttga aattgtattt tctgagacag tagctgactg caaagtactt
    38881 tctgtatcgt tttcaattcc tgtttctgaa gaactcgatg tctgagttgc gacagtttct
    38941 gtcgattctt cagctagcac tgttgtgttg gcaactgcta gatttcctaa aagaataacg
    39001 ctaaataata aattctttgc tttcatcttt tttctccttt gaattatgaa acttttccta
    39061 aataacagtg taactaaaaa agtaaaagca agtcaatgtc catatttaaa taacatttgt
    39121 ttctttttaa taaatttgct ttttctgcat aaaaagacgt aaatgaagaa tattcttctc
    39181 cattcacgcc ttaaattgat ggtcgtttga ataactattt aacaagtaat tctctctgga
    39241 tattatccgt tatggattat acttcctgtg tcgtcggacg gataatcatg ttgctgacag
    39301 acatccggtc tggcgtttct atcgcaaatg ctacagcaga agctatatct tcagatgtta
    39361 atccccattc tttttgcgct tcatgaagtt caagagcagc ctttttgtcg ctgatggttt
    39421 gatataattc agtttgaaca gctcctggag agatgatcgt agatttgatg ttattttgcc
    39481 gttgctcttg acgtaatcct tccatgattg cacggacagc aaattttgtt ccgcaataaa
    39541 cggcggaatc aggataaaca acatgtcccg ccacagaatc agttgaaatg atatgcccag
    39601 atttttgttt ttccatgatt ggaagtacgg cggaaattcc atttaacaca cccatgatgt
    39661 tgatatcaag catattttgc cattcatcac ggtgaccttc aactaatgga gcggttggca
    39721 tgattcctgc attattgtac aatacatcga ttcgaccgaa cttttccatc gctaaatcga
    39781 taactgcttg gacttgtgca aaatctctta catctgctgt ttgaatatat agctcagctt
    39841 ctggtaaaga ttcttttatt gcttttaggc gatcttctcg gcgtgcagct ataactagtt
    39901 tagccccttt ttctgctaat agcttcgttg ttgcttcacc gattccgctt gaagcgccca
    39961 tgatgacaat cactttttct gataaatcat tcattctatt ctcctcctgt tttgaaaaat
    40021 ctcttgctta tactcactat ctcttatttc atcttactgt tcaaataatc cgttcgacag
    40081 gtttgacgat tttggcaggg attcctgcaa cgatggtatt gtctgggaca tctttcgtaa
    40141 cagttgcatt cgctgcaaca atggcattct cgccaatcgt tacgcctggc agaatagttg
    40201 ctcctgctcc gatccaagca tttttcttga tgtggattgg ctttactgac aagcctcttc
    40261 gttcttttgg atcggttaga tggttcaccg tgatgaggtt gacacgagga ccaatcaata
    40321 ctgaatcttc taaacagatg ccacctaagt caacgaacat cacttgacga ttgataaaga
    40381 catttttccc tatactaata tgtttgccga aatctgtata aaaaggcagc agaacagtca
    40441 ccgtatcatc tactttgtca ctaatgatat tttctagccg tttgcggata tctgctggtt
    40501 catggtaccc agtattcagc gctgctaaag gctcttcgtt ttccgcttgg atctgatgga
    40561 tcacttcaaa gagcggatcc gttactcgaa tctctttatt gacgatttgt ttttcgatga
    40621 ggcttgtcat tttgctccct ccttttatcc ttactttata acggacggct tatgatgtct
    40681 aatacttata aaacatactt aactattcct tttaactata cttagtggta tgctttttta
    40741 aaaacaggag ggaaaaaatg gatattcgta tactgacgta ttttttagca gtagctagag
    40801 aaaaaaatat cagccgagca gcaaaatctc tgcatatcac gcaacctact ctctcaaaac
    40861 aactgaaaga tttagaagaa gaacttggtg tgacactttt cactcgaggc tcccgagaga
    40921 tacagttgac ggattctggt cgatatcttt attcaaaagg aaaagaaatc ttgtcgttgg
    40981 tagaaaaaac ggcgaacaat ttaacaaatg acgcaatcat ctctggagaa atctacattg
    41041 gcggaggcga aactaaagca atctctttca tttctgatag ccttcattcg ttgatcaagg
    41101 cgcatcctga tatccagttt catttatata gcgggaatgc agatgatatc atagacaaac
    41161 tggatagcgg attacttgat tttggactcg taatcgaacc gacaaacaaa caaaaatacc
    41221 agtcatttcg actgccagcc caagatcgtt ggggactttt gatacatcgg aatcatcctt
    41281 tagcaaagca aacagtcatc catccgcagg aactgaaaac tgttcctttg atcatttccc
    41341 ggcaaacgtc agtggacagt caactagctg aatggctagg tttcagcata gatcagttat
    41401 cgatcgtcgg aacatataac ttattgtaca atgcttcttt gatggtcgat aacgggactt
    41461 ttggtgcatt agctctagat gggatcatca atacttatgg gacggaaaaa gtattcatcc
    41521 cactcgatcc gccattacaa gcttcactga atctcatttg gaaaaaagac cagcctcttt
    41581 ctcctgctgc tcgtaaattt ttagatgtcc taacggagaa atacagttaa gtagagtttt
    41641 tttccttcgc tttcagcttt ttaacaacga ataagttaag caaaagatcc aatgattaaa
    41701 taacataagt ttcttaattt gttccttcct tttatttctg ataattccag taaattacta
    41761 taattataaa aaaagaattg acaaaacata actgtctata taaaattgat tacgttaaca
    41821 ataattcgtt tcatgtgact agatcatact aggcatgaaa aatctctgag gaacatggtt
    41881 tccctaggag gtttttcgtg tctatttttg tttggccgca atcaactggt ctagcaaacg
    41941 tgaaaaccaa atcttcaaaa agggtgcgcg catgaaaatc aaaaagatct ttaatcaaaa
    42001 tgcagtattg gtagatgacg gtggacagga aaaagtagct attggaaaag ggattggatt
    42061 tgacagaaaa agaaatgatt tgatttttga tcgtgatgtc gaacggatat ttgtcatgga
    42121 accagaaggt caaatcaagt tgcaaaattt acttaatcag attgatgaac gctttttatt
    42181 tgcagcagaa acgatcattg atcatgcgga aactgttttg atggaaaagt tgaatgaaca
    42241 tgtattgatc gctttgaccg accacattgc tttttctgcg gagaacatta ataacggtat
    42301 cgtcatccgc aacaaacttt taagagaaat cgaagtgctt tacagtgatg aattcagtat
    42361 tgctcaatgg gcagtagagt atctgacaaa agagcttggg attccatata cttatgacga
    42421 agcaggatat atcgcaatcc atattcacag cggacgaagc ggtcggaaca ataaccatcg
    42481 cagtatccgt gaagtgacga ttgtatctga catcatccat ttagtggaat cagaattgga
    42541 aacagatatc cattcagaac cattttcact gaattattca agactggtga atcatttacg
    42601 actgcttcta caacgaaccc atgcgcaaca atatgctgtt ttagacacgg aaatcgtaga
    42661 aatggtcaaa cgaaaatacc cagaaagcta taagatttct aaaaaaatcc gtgtgttgct
    42721 gaccaaagaa taccagctcg cgatcacgaa agaagaactg ggttacttgg ccatccatat
    42781 cgagcgttta agaagcgcaa tcgtacaaac aaatgttaac aatcatgagg aggaaaaaaa
    42841 ttgaaagcat atctacaacg aatgggacgt tcccttcaac ttccagttgc ggtcttacct
    42901 gctgctgctt tactagtagg gatcggaaac tggtgggcat cttatagtaa cgacattgtc
    42961 gctcattttt tacaagctgg cggtaacgca gttttaggtc aattaccttt gctgtttgcc
    43021 gttgggttag cactagggat gtcagtggac aaatcaggtg ctgctgcctt ggcaggtttg
    43081 gtcgcatttg aagttccagt aaatgtttta aaaactgatt cagtagctac tttattgaat
    43141 atcaaaccag aatcagttga tccatctttt gctcagatca gtaacgtatt tattggtatt
    43201 attgccggat tgattgcggc agctctttat aatcgtttcc acgaagcaaa attaccgatg
    43261 gctttatctt tctttagcgg caagcgtcta gtgccaatct tggctgcctt tgtcatgttg
    43321 ctgatcagtg cagtgctact gctcgtatgg cctccggttt actctgcttt agtttctttt
    43381 gctaaattca ttttgagttt aggctggggt ggtgcaggtc tttatggctt cttcaaccgt
    43441 ttactgatcc ctacaggatt gcatcaagca atgaactccg tgttctggtt tgacgtagca
    43501 ggaatcaatg atattggtaa atttttggct tctgaaggaa caaaaggcgt aacaggaatg
    43561 tatcaagctg gatttttccc aatcatgatg ttcgggcttc cagcaggagc atatgctatt
    43621 tacagaaatg cacgtccaga aaacaaagca aaaacagctt ccttgatgtt agcagcagct
    43681 tttgcttcat tctttactgg tgtaacagaa ccacttgaat tctcatttat gttcgttgct
    43741 tggccattat atgtcttgca tgcaattttc actgggattt ctttagcagt gagtgcattc
    43801 ttccattgga cagcaggatt tgcctttagt gccggatttg tcgattactt cctatctttg
    43861 aaaaatccga ttgcgaatca gccattgatg ttgatcgtcc aaggattagt aacagcagtg
    43921 atttactact ttggctttaa ctttgctatc aaaaaattcc atctgatgac tcctggccgc
    43981 gaggaagcag atctttcaga tgacgatact gcaacgacta attcagacaa taaatatgcg
    44041 gcacaagcaa gcaaaatcta cgctgcatta ggcggagccg acaatgtaac ttcgatcgat
    44101 aactgtacta cacgccttcg tttgcaagtc aaagatacag gcctgatcga tcaaaataaa
    44161 atcaaagcca ctggcgtacc tggtatgaag atcatcgacg gaaaaaatgc gcaagtcatc
    44221 gtaggtacag aagtacaatt cgttgcagat gagatggcaa aactacatgg tggtgctgca
    44281 acacgacctg cacaaacaaa caccgtggtt aaaacagaaa catttgccgg tggaacgatc
    44341 gaacaagata tttacgctat cgcaaatggg aaactgattc caatcactga agtttcagat
    44401 gatgtattcg cggaaaaaat gatgggtgac ggctatgctg tattaccaga aaatggagaa
    44461 atcttctctc caatcgctgg tacgatcaca aacattttcc caacgaaaca tgctgtcgga
    44521 atccaaacag atgctggtat cgaagtcttg cttcatatgg gcatcaacac tgttgactta
    44581 aaaggcgaac cattcacttt atatgtaaaa gaaggtcaaa aagtcgcaag aggtcaattg
    44641 attgctttag ttgatcttgc cgctattcaa tctgcaggca aaaacacgga tatggtcgtt
    44701 gtattcacaa atggcgacaa agtccaaagc ttagaaatcg aacccgcacg tgatgtaaaa
    44761 gcaaatgata aaatcggctc tgtttccaac aaagcctgag ccatcgtctt ccccagactt
    44821 ttttatcgat aaacaaacag gagctgagac aaaaatcttg tctcagctcc tttttccgaa
    44881 taaaaacggc gttctcctgc caactccgcg gtaataagtc cagccaaacg taacatatga
    44941 agagctattt tttatttgac tcttcttatt acttgatgca gaacagcttt tttacaacct
    45001 ctgataaacg gtgttcaaaa accagtttat cggtatgaag tcaaatttat gcaaaaatgt
    45061 ggaaagccat tttcacaaaa tttttcttca tactcaaaac taatcggctt tttcacaacc
    45121 tctttataca ttattcctga tccaatattt cttttaatgc tgcttcaaga gaagttaatg
    45181 gttttcccaa tactttttct aaatcatcag aggaaatagc taactgatcc tttttaatat
    45241 catgttggat cgctaaaaac atgtcagctg cttcttgagg gaatccggct tcttttagat
    45301 tttcgacaaa cgctgcatca cttgcttcaa taatttctaa ctttttacct gtcgcacgct
    45361 ctaatgcctt ggctaattct tcatagtcag ttagattgcc ggataattct aagatttctg
    45421 gagagtcact ttctactagt acttttgctg cagcttctgc atattcacgt ttcaatgccc
    45481 aacctgtttt tccatttcct gcagcataaa cgaattgacc agtttttaaa gcctgcccaa
    45541 tgataggtaa ttcattctct acataccaat tattccgtaa aaatgtatgg gaaatacctg
    45601 aatccttaat cattttttca gtgaattggt gatctggcgc taatacactg atcgagtgat
    45661 cggcatctgc aaaacttgta taagcgatat aagatacccc agctgcttgt gctgccttca
    45721 ctacattctt atgttcggct tgacgatttc ctggaacgcc agagacaaaa agcaatcgat
    45781 ctattccttt caacgcttct ttcattgagt ctaaatcatc ataatcacca atacgtatat
    45841 taaatccagc ttctttcagt gcttctcctt tttcttcact acgtactagt acatagattt
    45901 cattttgtgg aaccatcttt tttaagaatt ccagcgcata accaccaaaa cctccagttg
    45961 ctcctgttac taaataatgc atatttgcca ctccctattc tttttatttg ttaagattta
    46021 cagctgtaca tttataaatt acacctttat cataaagagt gtggtaagat aagtcaagca
    46081 aaaaaaatga aaggatgaca actgatgaag tattctctgc aatttagtga tgcaatccat
    46141 gtacttgcct atattgaaat gtttcaagat acccgatcac tttctagtga gatgattgcc
    46201 agtagtatcg aaacaaatgc agcaaatgta cgtaaaatca tgagtcagct caaaaaatcc
    46261 ggtttgatct ttacaaaaac cggaaaaccg cagccttccc ttgccaaaag tccagatcag
    46321 atttctttac tagatgtcta tcgcagcatc gaaggtaata cacagcttat ccaagtcgat
    46381 ccaaaaacaa atccaaattg cgtcgttggt gcgaatatcc aagaagcttt agaagaaagc
    46441 tacgctcggc tgcagaaaaa ggcagaagaa gaaatggcta agatcacact tgatagtctg
    46501 atacataaaa tagccgtttt ggaaaaagaa aaaagaccag aaaacacagg cctcgtagag
    46561 agatttttat aattctctct actgtgcctg catttttctg atcggaatcg tctacataaa
    46621 ttcttttatt ctccggggga caaaagcctt gtcatgcccc tttttcctct ctaatgctca
    46681 ggtcaaagcg ctgaagtcat gacgtcttga taaacggtgt tcaagtagct gaccctcggc
    46741 attagttctg aaaaatgcaa aatatggaaa gctatttccc atttttcctc tctaatgctc
    46801 aggtcaaagc gctactttca cgacctctat attatcatgg tttattgact accggtatcc
    46861 agatttctcc catatatttc ccgttggtaa ctcccatttc gactacagca tttggacccc
    46921 caacataggc gtaatcttct acttctctta aaacatgccc aaaggccgta ttcgtcaaat
    46981 catttcttaa ttcttcaggc gattctgcta aagcgattac aacaagatac gttccttttg
    47041 ggaatgggat gattcgggta gtgtttggaa catcctgact cgtttgaaca ccgacatagt
    47101 acatcatttt cccgttatag ggttcgttga ctgtaaagaa ataatgattt tcagcagctt
    47161 gtttcaatgc atctattgtt ccattaccga taactttttg ctcaaatgct tgtttctctg
    47221 cagccaaacc gacaaaatcc gtattgctac tcttcaattc cacaccaata cccgtcaccg
    47281 taaaatctgg tttttcttcg atcctatact ttgccatttc tcccaccctt tctattatat
    47341 taagttcatc ttacataaaa ttaaagggaa atgacacgat tacgctcttt tttctaggga
    47401 tatttttatt ttttaactat ttttttgctt tcactgcaat ccaaatctca ctgtaaacat
    47461 tactgaaatc ctctccccat tttgtaaaag agatctcggg agcttcgact aactcataat
    47521 ctgaagaagg aagccattct gcatagattt tcccccaagt atcttgtaaa gtttggggaa
    47581 atggcccttt attaggaaaa atggcccaag tgtgttcttc tatagcaagc atttctaaat
    47641 cagcgtacgg attttctttt gtcgtagcaa agccaatcat gtgcatcaaa ctgccctttt
    47701 cttccaagcg ggctccatca aatccataag aaacgttgat tacttgattt ggatataaat
    47761 ctcctaactt atgcatctct tccctttgag tctccgtgat cgtctgcgct aacttttgta
    47821 tttcttgatt gactccttca aatttgacag gaactttttt cgccacacct gccacataaa
    47881 actgttcttt cttttcgata cgaaattcca tcgattttcc tcctctgaca tcaataaaaa
    47941 atgtcaagcg tggaaaagat ttttgtatgc cgtatctgct gatttctgaa ggcagatatc
    48001 ctgaccaatc acgaaaagct cttgaaaaac cttcgactgt ttcatatcca tatctaaacg
    48061 ccgtttctgt cactcttgat ccatctagga gatcgcgatt agccaaagcc aatctacggt
    48121 ttttgatata gtccattagc gacatccctg caataaacgt aaagatgcgt tttaattgat
    48181 attcagagat tcctgtattt tcggctatta ttttatagga aagtccttct gtcaaatgtt
    48241 cttcgatgta atcgatcaat tggttcagat ctttaagcat atttatcttc ctccttgctt
    48301 gcctttttat catattatca aagaaagtca attacttgat gaatcaggaa ccaatttagt
    48361 cagatgtaga aaatgttgta tggatctaaa gaaaattctc aagcagcgtt tcgtaacgga
    48421 taattggtat tatctgccta tctatgtttt acctttagaa aaataaattt tggggaagac
    48481 acattctaag taaacaaagc ggtaaaaata tcttttattc ccttagttaa tccgcttact
    48541 acctactgta caaagcaaat tgttgttcaa actgctgatc agaacgctgc ttggattttt
    48601 ctttgcgata catcatacct cttttatcaa ttggaacaat tatttatttc taacaaaatc
    48661 acttcatttt tgtcagataa ataaaa
//
LOCUS       DARBET010000014        45695 bp    DNA     linear   BCT 07-NOV-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4027
            SAMEA114569841-rid20112183.denovo.014, whole genome shotgun
            sequence.
ACCESSION   DARBET010000014 DARBET010000000
VERSION     DARBET010000014.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA114569841
            Sequence Read Archive: ERR12203666
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 45695)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 45695)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001972618.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001972618.1
            Genome Coverage       :: 132x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/07/2023 13:18:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,041
            CDSs (total)                      :: 2,975
            Genes (coding)                    :: 2,908
            CDSs (with protein)               :: 2,908
            Genes (RNA)                       :: 66
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 67
            CDSs (without protein)            :: 67
            Pseudo Genes (ambiguous residues) :: 0 of 67
            Pseudo Genes (frameshifted)       :: 26 of 67
            Pseudo Genes (incomplete)         :: 28 of 67
            Pseudo Genes (internal stop)      :: 20 of 67
            Pseudo Genes (multiple problems)  :: 6 of 67
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..45695
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA114569841-rid20112183.denovo.014"
                     /isolate="VRE4027"
                     /db_xref="taxon:1352"
                     /country="Denmark"
     gene            34..657
                     /locus_tag="SCJ86_000891"
     CDS             34..657
                     /locus_tag="SCJ86_000891"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014387005.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEG1168472.1"
                     /translation="MFVIWEVVKSRVQEMNEHFSVSFAENPSKYFRRLVFLLILSLLL
                     TVAMFLLPEKIREEQVNSLVYSSIPTSKLKPFDYSKADQKIKQSRAISVMFSKPNGKT
                     YQDMLDVFNDPELMEELNRPIFYYPIVYDVHELEQKYNIRTDEVTFIFFDGGKEANRI
                     TAGKGGITDFDKELIPELNRLPLANIKQLEEELDRTGVTSQNSEIAN"
     gene            1107..2156
                     /locus_tag="SCJ86_000892"
     CDS             1107..2156
                     /locus_tag="SCJ86_000892"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309296.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HEG1168473.1"
                     /translation="MKNKRLITVVVIILVYLAGAFIYGKASGEKESTSTNQTKKTTVV
                     GVLQYVSHPALDEIYRGIKDGLKEEGLKEGENLEIKFQNGQADQSKLATMSQQLVQSD
                     PDALIGIATPAAQSLANTTSSIPVVLGAVTDPVGAGLVASLEKPGGNVTGVSDQPPVT
                     SQIKLASDLLPNAKKVGILYASSEDNSKYQVAQAEEAAKKEGLESVKYAVPSTNEIAQ
                     TVQVMSRQVDFIYVPLDNTIANAMQTVVKEANKANIPVIPSVDTMVEQGGLATIGINQ
                     YQLGLQSGKMAAELASGKEKPETTPVYTFDQGDTVINQSQADRLGIIVPQSMKEKAKI
                     ITDDSQQETSKEDDK"
     gene            2153..3034
                     /locus_tag="SCJ86_000893"
     CDS             2153..3034
                     /locus_tag="SCJ86_000893"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345092.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEG1168474.1"
                     /translation="MIVSAISQGMLWAILGLGIFMTYRILDFPDMTTEGSFPLGAAVC
                     VTAITHGVAPIVATLLGVGAGMIAGLVTGLLYTKGKIPVILAGILVMSGLNSVILFVM
                     RTPNLSLLNHPILQDSFHRFGLPDYFDTVLLGFISLGILIAVLLFFFNTDLGQGYIAT
                     GDNETMARSLGIKTDRMKILGLTLSNGVIALSGALIAQNDGYADVNKGIGVIVIGLAS
                     IIIGEVIFQELTLAERLIAIVVGSIIYQLLILVVIKLGFDTTYLKLFSAIILAICLMI
                     PQLKKALNLKTGFEKEA"
     gene            3037..3807
                     /locus_tag="SCJ86_000894"
     CDS             3037..3807
                     /locus_tag="SCJ86_000894"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HEG1168475.1"
                     /translation="MTDILTIKNGRKVVNNGMNEEKVLLDEINLTIREGDFITVLGGN
                     GAGKSTLFNTIAGTLQLSQGSISFKDQLITKDSEEKRARFLSRVFQDPKMGTAPRMTV
                     AENLLLAQKRGQKRKFRLRNLKNQKEDFFRLCQEVGNGLEQHLDTPAGELSGGQRQAL
                     SLLMATITAPELLLLDEHTAALDPKTAKALMRLTNQRINEQKLTCLMITHRMEDALKY
                     GNRLIVLQKGKIVQDISEKEKQHLTMGDLLQFFEEVVD"
     gene            4086..4940
                     /gene="aroE"
                     /locus_tag="SCJ86_000895"
     CDS             4086..4940
                     /gene="aroE"
                     /locus_tag="SCJ86_000895"
                     /EC_number="1.1.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313777.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="shikimate dehydrogenase"
                     /protein_id="HEG1168476.1"
                     /translation="MISGQTKLAGLFADPASHSLSPLMHNTAFEANNIDAVYLAFQVN
                     PMNLKQAVESIRTFDMLGVNLSMPNKTAVIPYLDEVSQEAQLIGAVNTIVHEKGRLIG
                     YNTDGAGFMQSVQEAGISIKKKKMTLLGAGGAAKAVTVQAALDGAEEIVVYKRNNQTF
                     AQVKETFEQLSYRTSCKIKVRDFADTHQLRKDLKESCLLVNATDIGMGKKINQSPLLD
                     ISMMHENLAVFDLIYAPRETRLLKEAKKVGAKGYNGLGMLIYQGAIAFELWTKEKMPI
                     HVIEKLFD"
     gene            5118..6281
                     /locus_tag="SCJ86_000896"
     CDS             5118..6281
                     /locus_tag="SCJ86_000896"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LCP family protein"
                     /protein_id="HEG1168477.1"
                     /translation="MSRMDRYKDIHKKAKPLKRDTGSIFARRERKRTRMENEEDATRI
                     YEREQHSFEHLNQYEQQIYRDAPRAEEQQKNKKFFNRQKKNKPLKPRRKRSWKKIILS
                     ILLVLILYSGISFFIGKYVAEHDTSLEAATSETFHGQKATNGAHNILILGSDTRGEDA
                     GRADTIMVLQLDGPSHKPKLISFMRDSFVTIPGVGQNKINSAYAYGGADLVRQTLVEN
                     FGIDCQYYAKVDFKSFEKVIDALFMNGVKIDAEKDLNLDGVDIKKGVQKMDGHVLLQY
                     ARFRMDEQGDFGRVRRQQQVMNAIFSQLKNPLNLIRAPYAAGKALGYTSTDVTAWFLT
                     KNLFSIARGAGGIDRISVPVDGSWNYGQSDYAGSILEIDNQMNQSAITDFLNK"
     gene            6398..6808
                     /locus_tag="SCJ86_000897"
     CDS             6398..6808
                     /locus_tag="SCJ86_000897"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340634.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HEG1168478.1"
                     /translation="MDIYLNFKNESREAIAFYEKVFQTEASGITTYGEMPGIEQMNLP
                     EEAKNLITNARLNIYDTLVMFADTPEGIGPELVVGNNVSLVIQTRNEEETKRLFESLA
                     EGGKIEMPLDAVPWAQLYGMVTDRFGINWQINQE"
     gene            6982..9210
                     /gene="topB"
                     /locus_tag="SCJ86_000898"
     CDS             6982..9210
                     /gene="topB"
                     /locus_tag="SCJ86_000898"
                     /EC_number="5.6.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345093.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase III"
                     /protein_id="HEG1168479.1"
                     /translation="MKTVILAEKPSQAKAYAETFQQQKRHEGYYEISDPLFAGEVTIT
                     YGFGHLVDMVAPGAYEERWAKWSLENLPIFPETFRYEVPMDKRAQFSIVKRELQSADT
                     IIIATDGDREGEAIAWSIIRQANAFSKEKNYLRLWINSLEKEAIYEGFKNLQPGKNYF
                     PKYKEAQARQNADWLIGMNGSPLYSLLLQRKGIDGSFSLGRVQTPTLYMIYQLQEEIK
                     HFKKETYFEGEGLITTNKGQFSGKIVPKKAFKTQEELTKEIEKLGAHLGKQNGRILEV
                     TKKEKRLHSPRLFSLSSLQTKMNQLMKASAKATLDAAQGLYESKFLSYPRTDSFYITE
                     NEHQYLVKHLQNYKKFLGIEEVQTTEIKAKKRYVDAKKVQEHHAIIPTKTIPTPARFA
                     KLSKLQQAIYLQVLRTTVAMFAADYIYEETTVMTGVQQLKLKSIGKIPLQQGWQEILR
                     IKSKKKEAQTLPPIVENEEAEVELKSMEKETQPPKPYTEGTLITAMKTAGKTLDNDEA
                     QEILKEVEGIGTEATRANIIENLKQKHYIEVNKNEITVTPKGITLCKAVANEPLLTSA
                     EMTARWEGYLRKIGKQEGTPSVFLENIKKFILHLLAEVPNQIDKVDFSDEISGTRQIK
                     AMEKKNDQLGRCPKCKKGIVMLYPKIATCLNPECDFKLWPTVAKKKLTKTNLRDLLSK
                     GKTSKVVKGFTGKKGKFDAVLVLKEDMTIGFSFPWIENSGTKAVDGNKETSRKNNENE
                     VE"
     gene            9381..9665
                     /locus_tag="SCJ86_000899"
     CDS             9381..9665
                     /locus_tag="SCJ86_000899"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEG1168480.1"
                     /translation="MGAITIVAVYQAFLKYPDYGTKLLSLTHNQTAYSPTLILSTLTA
                     GAKSATYFFHKTTSMLKRKKQRKQLLVVVAAMIIIYFLPVITQVFHQYFG"
     gene            9825..10061
                     /locus_tag="SCJ86_000900"
     CDS             9825..10061
                     /locus_tag="SCJ86_000900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318769.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AbrB family transcriptional regulator"
                     /protein_id="HEG1168481.1"
                     /translation="MKKMKKYKIRKSGNSDVTTIPPEVKEFMGVQTGDAISYVFQSDG
                     SVRMIKAQEEPDIDSLVDSIMNQYEDALKDLVDL"
     gene            10058..10456
                     /locus_tag="SCJ86_000901"
     CDS             10058..10456
                     /locus_tag="SCJ86_000901"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system death-on-curing
                     family toxin"
                     /protein_id="HEG1168482.1"
                     /translation="MRYLSTKEIIKINAKVILRHSPGEMIGVKDANALDMAVKQPSQA
                     VFNQELYPEIYEKAAILAINLAKKHPFHNGNKRTALVAMLLFLQLNNCPVAFSRQEAV
                     DFIIMITTSSLDFDSLKSKITSYLRQTAIK"
     gene            complement(11123..12067)
                     /locus_tag="SCJ86_000902"
     CDS             complement(11123..12067)
                     /locus_tag="SCJ86_000902"
                     /EC_number="1.1.1.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010750986.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-lactate dehydrogenase"
                     /protein_id="HEG1168483.1"
                     /translation="MKKTSRKVVIVGTGFVGTSIAYAMINQGISNELVLIDVNQEKAE
                     GEALDLLDGMAWGDENVAVWSGGYEECKDADIVVITAGINQKPGQSRLDLVKTNASIM
                     RQIVKEIMGSGFDGIIVVASNPVDILTYIAWNESGLPTSRVIGTGTTLDTTRFRKEIA
                     LKLKVDPRSVHGYILGEHGDSEVAAWSHTTVGGKPVFEIVEKDHRIAQDELDVIADKV
                     RNAAYEIIDRKKATYYGIGMSTARIVKAILNNEQAVLPVSAYLTGEYDEKDIFTGVPS
                     IVDENGVREVVELSINEEEKAMFSKSTSALREVLNTVL"
     gene            complement(12363..13307)
                     /locus_tag="SCJ86_000903"
     CDS             complement(12363..13307)
                     /locus_tag="SCJ86_000903"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296705.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HEG1168484.1"
                     /translation="MRNEESIETLQREQGMNINDLNLAYHLQTPPKIHRGDLFQQGNI
                     HISKHRRYAEVPEHTHDFVEINYMYSGTCLQIINGERIEMEESDVVIIDKEATQRIEY
                     TGQEDILINILLKEETLSTEILNHLASTNNLLSSFLINASKEKGEHTQFIHFRGGKTS
                     RLTHLIENMLLIYFDTYSHKIRSLNLYTSLLLIELTHLLEQQSFSSADGCAENEMLRV
                     LDYIDKHFTSCTLKETAAHFGYNPVYLSNKLKMYFDASFQELVLKKKFDFALELIQET
                     SFSLEEIANRIGYKQTTSLFKLFKKYSTCTPNEWRNKH"
     gene            13527..14741
                     /locus_tag="SCJ86_000904"
     CDS             13527..14741
                     /locus_tag="SCJ86_000904"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320890.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEG1168485.1"
                     /translation="MLMNKKMMKFSLLSVSLILTSAGAVTSAIPSMSATYATRSLSAV
                     ELLTTAPSFMVTIFVLISSFIARKIGQKQTVIIGLVIASICGILPLFSQSYSIVLASR
                     AGLGIGFGLINSLAVSMIAAFYHGDERASMIGFQSAFQGFGAALMTFVAGQLVQFGWQ
                     YTFLVYAISLPILVLFVLFVPTPETTELQSHGTAPTRQVIKTNPKMISYGIFLLLVIV
                     VYNAVSIKLATVLTTYSLGNETNAANILTIMQLASMAAGVCFGWLQTKAHRYMLTLSL
                     LLMGIGFVLIATVINLYVIGFGAILTGISFSLFIPYLFNQVSIQAPKYAESFNTSILL
                     VGANLGTFIAPYGLLFLSFASLGSKTLAVFVNGSILLLISALISLLVVHRTNRKTVIR
                     VDEEIKEQLTTK"
     gene            14763..16778
                     /locus_tag="SCJ86_000905"
     CDS             14763..16778
                     /locus_tag="SCJ86_000905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="family 78 glycoside hydrolase catalytic domain"
                     /protein_id="HEG1168486.1"
                     /translation="MTNEKHKDRWLWYPGDFEIRHGLLQNFQREERGFDWPAYWYMDD
                     CHRNVKFKRYYFLDQPSMFKVTIQGVGYVEINGQKHPCGKWLTCPAGKAKIRIFVGHT
                     SGLPAMFIEGDEVKSDIGWTASNFIEEYPAGWSPLYVDIAKDPNQIYYQKECVHPVTE
                     TEIGGGILFDFGRAVNGCVELISLDDHIESITLCYGESDVEAMDIDYCYYKQIGMTRK
                     MKARKRAFRYLFIPEVSTGMVQLQVFHEFLSLSKQAAFSSDSSLLNQIWSVSEEAFKL
                     CSDLFFIDGIKRDRWIWSGDAYQSFMINQYLFFDEEINKRTILALRGQNEIKQHMNTI
                     VDYSILWLIGIENHYMMTKDYEFLRIVYPKMQSLMRYLMEQTNELGFIYGRAKDWIFI
                     DWSEMDKEGTLAAEQVLLLKAYHSMITCGDILAETYGENENYHTHLYQEKYQLLKQNL
                     FDYFWDEELGAFIDCYESGRRNVTRHANIFAVLFDFANKDQQQSILKNVLLNDTITQI
                     TTPYFKFYEQDALCKLGQTNIVYQTILDYWGGMLSHDAVTFWEEYDPTQTGNEKYSMY
                     GDPYGKSLCHAWGASPIYLLGRYFIGLYPTKPGYEEFMIQPNLDSFETLNCTLPIKDG
                     KISLEKTKDKIVITSDRTGGTVKMNGKSYPLLAKQPLVLTSYSKINA"
     gene            complement(16861..18234)
                     /locus_tag="SCJ86_000906"
     CDS             complement(16861..18234)
                     /locus_tag="SCJ86_000906"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336987.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS ascorbate transporter subunit IIC"
                     /protein_id="HEG1168487.1"
                     /translation="MDKLLNLFVSIVSQPSILVSLIALLGLVLQKKKFSEVIQGTIKT
                     FVGFLVLIGGAGLISNSLTPFASMFQTALHTQGVVPSNEAVVAIALQQYGTPTALIML
                     VGMIVNILLARFTRYKFIFLTGQAMLYVSCLTAVILVSAGFTANLWMILLGGVFEGTL
                     LTITPALCQPFMKNITGNNQVALGHTGNIGYASSGWIGKFFGNKDRSTEDIKIPKSFS
                     FLRDSTVSIMLLMSIVYVALALLAGPGFVERELSNGDNAVIFALIQAGTFTAGFVIVL
                     QGVRMVLAEIVPAFQGIAKKLVPDSKPALDVPIVFPYAPNAVLIGFFVSFFVGTLSMF
                     AMIGLQTTVIIPGVVGHFFCGAASGVFGNSTGGRRGAVLGSAFNSLLISWLPLFILPV
                     LGDLKIASSTFADTDYLVPGLVLGKLGEYGQIALTVGIIAFFLLVVLSSFLFAGKRTN
                     IVSKEQN"
     gene            complement(18260..18544)
                     /locus_tag="SCJ86_000907"
     CDS             complement(18260..18544)
                     /locus_tag="SCJ86_000907"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315031.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HEG1168488.1"
                     /translation="MKIFAVCQSGLGTSFMVEMNIKQALADLGADVDEFEVSHTDVGS
                     VSSDMADYFFIEHSLKDTLTSIPDEKLVPLQSIIDSEEVKEKVTKILSKE"
     gene            complement(18546..20561)
                     /locus_tag="SCJ86_000908"
     CDS             complement(18546..20561)
                     /locus_tag="SCJ86_000908"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BglG family transcription antiterminator"
                     /protein_id="HEG1168489.1"
                     /translation="MNYKISYLLKTPREISLEKAAEIIEVNTSEAIKQLTPFAEYVDD
                     TRIRIKKFRAIDWAEKFLDITRFEAVYTEQERRDLLYLMVYSTATSVSVFHFQDFLQV
                     SKGTVLSDIKKIRNELEVEDCQLSYTRKTGFRLLGSEAILRREAKNRTARLMQSLAGR
                     FGLTYLTATLSIDYYAKVRDLLDKTISLSDLRFAPNRMDELAYYLSLTTIRIGKTSME
                     LVKDAELLASLAVTDISRQVLTGLGYSEHQNSEIIFFSLCLASIVEGDVQEPALDFLL
                     ECSSEVIHRMEFLMAVEFASFRELLMNVFYHLVPAYFRISYSFYLPNVMIDQIKHQYA
                     SIYEMTRKALLPLERRIGKSIPEEEIGFFTILFGGEIRKADAEERNRKIRAVIVCPSG
                     ISSSLILKSELQQLFPMIFFTETNSADRLHEVDETSYDIIFSTVPLTLTDKKKSLYVL
                     HPIMTDLEKNQLINQVQRDWLIPGFSMPSAQELLSALLPYIELKEGVDEEKLYQVLNR
                     KMNKLLEKKEDQRPMLSELLTEEMIQLSDQPKNWQEAITFAAQPLLSENKIEESYIKA
                     MIERVEQYGAFIHIGDHIALPHARPEDGVNEVGMSLLKLQQPVDLLDNPAHPISLFIC
                     LAAIDNEAHLRALANLTKLLSNKQNLQDLLHASTKEEILAIIRKGDE"
     gene            complement(20740..21045)
                     /locus_tag="SCJ86_000909"
     CDS             complement(20740..21045)
                     /locus_tag="SCJ86_000909"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286049.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rRNA processing protein"
                     /protein_id="HEG1168490.1"
                     /translation="MTNYDGEKHKRLRGSMAPKTEEEVALAGTNSGETEDKEESLAGT
                     NSGETENKETALSGSNSVEEETKPNEPNHFEKDIEKAVEDVKEKFEELTSKNKHDHH"
     gene            complement(21134..22165)
                     /locus_tag="SCJ86_000910"
     CDS             complement(21134..22165)
                     /locus_tag="SCJ86_000910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306737.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HEG1168491.1"
                     /translation="MLTIAYLGNGKSTNRYHLPFSRKLTNKINIKKIFSPSEPVWKTF
                     EDIEYTDQLSDLWDDPEIQLVVITTPSQFHYEYGKMALEHGKNVLVEKPFAETSKEAE
                     ELFALAKEKGLFIQCYQNRRFDSDFLTVQKVIESGKLGDILEVEMHYDYFRPEVPENT
                     HEFSTANSYLYGHACHTVDQVLSYFGEPDDISYDVRQLLGKGRMNDYFDLDFFYGNMK
                     ISIKSSYFRIKERPSFVVYGKKGMFVKQTKDRQEEHLKMFYMPDHEDFGIDLPEHYGV
                     LTYIDEQGSYHEEKVISEVGDYSRVYEGIYETLVNGAEKVIKDEETILQMKILEEGIK
                     SIQESEGKI"
     gene            22372..23247
                     /locus_tag="SCJ86_000911"
     CDS             22372..23247
                     /locus_tag="SCJ86_000911"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MurR/RpiR family transcriptional regulator"
                     /protein_id="HEG1168492.1"
                     /translation="MLIEEKLTKQELFTTTEKRIVDYIRRNIEAAVYMTIEELAKATY
                     TSHSAIIRLCKKMGFSGFKEFRFELGREVHQLIAQFNQTIDPNFPFSSEDSTQQIAKK
                     MADLSIQSIKKAQLQIEHEDLDQIGKVLNKAQRIFLFAKGDSQITARKFQNKMVKLNK
                     FLIMAEEYSDSSWNAANLTSEDCAIFISYSGRIHHYERIMTYLKHVEAPTLLITGNQH
                     SEMAKQADMCLVISQEEYDFAKVATFSSQIAFDYVLNTLFSVIYSQNFEENIRNLKNK
                     QELMQKGLLKEYRKT"
     gene            complement(23399..24241)
                     /locus_tag="SCJ86_000912"
     CDS             complement(23399..24241)
                     /locus_tag="SCJ86_000912"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338272.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class C sortase"
                     /protein_id="HEG1168493.1"
                     /translation="MKPKKRKLLDLLMLFLLLSGIGILAYPFVSDALNNYLDQQIISH
                     YQQQAVKENEEVMAKIQENMTKKNQQLAKEGGNPGADPFTKKKEPAKKDRTYFEKHTI
                     GILTIPEINVHLPIFDETNTVLLEKGASLLEGTSYPTGGENTHAVISSHRGLPQAKLF
                     TDLPKLTLGDSFYIEINGRTLAYQVDQIKTVEPTDTKDLRIEKGQDFVTLLTCTPYMV
                     NSHRLLVRGHRVPYHAKEVQKEVQKVSENKRFFLYGLLAACILIFLLLIYLFRQSFKI
                     RNRS"
     gene            complement(24333..26210)
                     /locus_tag="SCJ86_000913"
     CDS             complement(24333..26210)
                     /locus_tag="SCJ86_000913"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344222.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SpaH/EbpB family LPXTG-anchored major pilin"
                     /protein_id="HEG1168494.1"
                     /translation="MKNHKKINVMLGVLFLILPLLTNSFGAKKVFAEETAAQVILHKK
                     KMTDLPDPLIQNSGKEMSEFDQYQGLADISFSVYNVTQEFYAQRDKGASVDAAKQAVQ
                     SLTPGTPVASGTTDADGNVTLSLPKKQNGKDAVYTIKEEPKDGVSAAANMVLAFPVYE
                     MIKQADGSYKYGTEELDTIHLYPKNTVGNDGTLKVTKIGTAENEALNGAEFIISKEEG
                     TPSVKKYIQSVTDGLYTWTTDQTKAKHFITGHSYDIGNNDFAEASIEKGQLIVNHLEV
                     GKYNLEEVKAPDNAEMIEKQTITPFEILANSQTPVEKTIKNDTSKVDKTTPQLNGKDV
                     AIGEKIQYEISVNIPLGIADKEGTQNKYTTFKLIDTHDAALTFDNDSSGTYAYALYDG
                     NKEIDPVNYSVTEQTDGFTVSVDPNYIPSLTPGGTLKFVYYMHLNEKADPTKGFSNQA
                     NVDNGHTNDQTPPSVDVVTGGKRFVKVDGDVTSDQTLAGAEFVVRDQDSDTAKYLSID
                     PSTKAVSWVSAKESATVFTTTSNGLIDVTGLKYGTYYLEETKAPEKYVPLTNRVAFTI
                     DEQSYVTAGQLISPEKIPNKHKGTLPSTGGKGIYVYIGAGVVLLLIAGLYFARRKHSQ
                     I"
     gene            complement(26207..27628)
                     /locus_tag="SCJ86_000914"
     CDS             complement(26207..27628)
                     /locus_tag="SCJ86_000914"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286054.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SpaH/EbpB family LPXTG-anchored major pilin"
                     /protein_id="HEG1168495.1"
                     /translation="MKKLGWLSMCLFLLLFKPAFTQVATETETEMVQITLHKLLFPNG
                     QLPKNHPNDGQEKALLQTYRGLNGVTFQVYDVTDSFYHLREKGKTVEEAQAEIAKNGA
                     SSGMFTAEATTTTLNNEDGIASFSLAAKDQEKRDKAYLFIESKVPEVVKEKAENMVVV
                     LPVHGQNNQKLSTIHLYPKNEENDYPDPPFEKVLEEPRNDFTIGEKITYSLHTTIPVN
                     ILDYQKFELSDSADEALTFLPNSLTISSNGEKLTEGFVIHKKPHGFDVLFSIPSLEKY
                     AGKKLTISYQMQLSSTAQANKEINNNGTLDFGFGVSTKKVSVYTGSKQFVKIETNKPD
                     KRLAGAVFLIKNKAGNYLQQTANGYKWTKNESDALHLISDKNGAFSISGLKTGSYRLK
                     EIEAPSGYILSETEIPFTISTFLSEDKEADSILKVVNKKENSRPFLPKTNETKNTLLG
                     VVGMVFASFAIWLFIKKRTGVKK"
     gene            complement(27631..31020)
                     /locus_tag="SCJ86_000915"
     CDS             complement(27631..31020)
                     /locus_tag="SCJ86_000915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331773.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VWA domain-containing protein"
                     /protein_id="HEG1168496.1"
                     /translation="MTTTGKKLKVIFMLIILSLSNFVPLSAIADTTDDPTVLETISAE
                     VISDQSGKKALNIKLNANNTSAEKIEKEIGLVENYLSDVERKEGDGYAYQVNSGKITL
                     EISSNTKQTIDLSFPIDPALYHSQANKLIVDNKEYDIIDETENKKDTDVSVPKPDEIE
                     EESSKENENSVSPFTLPTLSLPAVSVPSNQTIPTEYTTDDQGTYPKASWQPTGNTNVL
                     DHQGNKNGTNQWDGINSWNGDPNDRTHSYIEYGGTGNQADYAIRKYAKETSTPGLFDV
                     YLNARGNVQKDITPLDLVLVVDWSGSMNDNNRIGEVKIGVDRFVDTLADSGITDKINM
                     GYVGYSSEGYSYSNGAVQMGSFDSVKNQVKSITPSRTNGGTFTQKALRDAGSMLSVPN
                     GHKKVIVLLTDGVPTFSYKVQRVHAQSSSNYYGTQFSNTQDRPGNTSLISRIYDAPDQ
                     NNLSRRIDSTFIATIGEAMALKERGIEIHGLGIQLQSDPAAGLSKAEVESRMRQMVSS
                     DEKGDLYYESADHATDISEYLAKKAVQISATVSNGQINDPIAEPFIYQPGTLSVKSVG
                     TSPTTVTPSISIEGNTIKSNQIYLGKDQEIQIHYQVRIQTENEDFHPNFWYQMNGRTT
                     FQPNIDTNELAEFGIPSAKAPGVSLHIKKLWEEFDNNLADRPDQVTFEIQREHTTNAA
                     AWKNGYIRIIKPAKDTTNTWERADIDKLSANSGESYQEILSLPQYNNQGQAFSYQTIK
                     ELPVPGYDSQQIDAMTWKNTKQFTPLNLKITKNSSTGEKDLIGAVFKLTGDSIDTLLT
                     DHGDGTYSLPENVKLQKEMTYTLTETKAPEGHGLSKKTTWEIKIASDGTVTIDGKTVT
                     TSDDTIQLTIENPFVEVPVAVRKYAMQGTDKEINLKGAAFSLQKKEANGTYQPIDSQT
                     TNEKGLASFDSLTPGKYRVVETAGPAGYDTSPGNYEFQIDKYGKIIYTGKNTEMTNNV
                     WTLTHQNRLKAFDLTVHKKEDNGQTLKGAKFRLQGPEMDLESPKDGQETDTFLFENLK
                     PGTYTLTETFTPEGYQGLKEPVTIVIHEDGSIQVDGQDHESVLSPGAKNNQISLDITN
                     QAKVPLPETGGIGRLGIYLVGMIGCAFSIWYLFLKKERGGS"
     gene            31277..32668
                     /locus_tag="SCJ86_000916"
     CDS             31277..32668
                     /locus_tag="SCJ86_000916"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002351559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HEG1168497.1"
                     /translation="MEKIIRILSISDKWMTKKELAKRIGSSESTFVRYIEEIKIRWGT
                     DVTIQSSQKLGYRLEHLNVPAYLNVLMEMSQSSTNSRLLNEIIVNPGKTIEYYCEKIS
                     ISRSSFTRKLKQCNQILESYLLKVIVDQGFQLTSLKSELQLRIFVTFFFLSYYGRRTL
                     PFPIEKKKIKEIMVKNNCQMNLFQEESPYEQSFFIMYFVVHLMRESQGTPFEWKAGDS
                     YVQLINIDKKDFLILKNLLGKLKYSSYIKVVNYFVNTIFSEFSEKEKNEISEQIRKNM
                     SYCSFSSADCKEKNEEYVVQLLTQIYFFSRIFPYDTTHLTIRATYFAQQMQHRQIRLY
                     AGFRAELQFYSTLLNQNLIYYLSSYIFWMLIGNPEIHHYTSDKKILIISDLSLRHSQY
                     IEEYISDILAVHKIHSETTAITEDQLSKYNLLEYDLIVTNQPILNAHVPHILIDDSVS
                     FANEEELIRLFEL"
     gene            complement(32741..33388)
                     /locus_tag="SCJ86_000917"
     CDS             complement(32741..33388)
                     /locus_tag="SCJ86_000917"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328434.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR family transcriptional regulator"
                     /protein_id="HEG1168498.1"
                     /translation="MTMKGYPRIIRDKGEMWMPKETFFNLSNAKKQRIDDLLLEIFCS
                     QHISQVKVSEIVDKMGMSRGAFYKYFQDLEDAYLYTIKSSSHIVHQDILKFISENKQH
                     FFLGIEKYLEWCCDLDHESIYWQRLYLLTQRNDQQTTKRRELSADSPMNEKWLELLRI
                     NHFSIQSSEEAVSFLYFSMTLVMNTLTDLIANNWTKEELLKDFRYKSRWIAEGIQ"
     gene            complement(33396..34082)
                     /locus_tag="SCJ86_000918"
     CDS             complement(33396..34082)
                     /locus_tag="SCJ86_000918"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002366355.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEG1168499.1"
                     /translation="MKKILTMKKVTKTFGSGHTKVSALKAIDFYVKEGEFVSIIGPSG
                     SGKSTFLTISGGLQTPTSGEIVINGHSLTELPEKKRADLRFKEIGFILQSSNLIPFLT
                     VKEQFELVDQVTSKKEDSTKIDSLLQSLDIQSLKNSYPKDLSGGERQRVAIARALYNE
                     PSLILADEPTASLDTDHAYEVVKLLVKEAHEKQKATIMVTHDVRMIQWSDRVYRMEDG
                     YLTEEEKKKE"
     gene            complement(34088..35161)
                     /locus_tag="SCJ86_000919"
     CDS             complement(34088..35161)
                     /locus_tag="SCJ86_000919"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286061.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEG1168500.1"
                     /translation="MFLAIKEIRYSKLRYGLIVGIMFLIAYVVFMLSGLANGLSEEFK
                     KAIDDWDAQEIVLSEDANQVFAASQLTRGDLKYIEGGEKAPIGLYSGAIKGKNKENVT
                     VFGTTKEAFLLPKLTKGHEFKTKNEIIISQNLADSGYKIGDTIKIGSYDDPLTIVGIF
                     PETYYTVSPVIYASLDTWTALKYGQQPFASEEEQPINGIVIKDSAKISKEKASKGLQL
                     LTIGTFIESIPGYSAQNMTLNAMIYFLFLVVAAVVGIFMYVMTLQKTAIFGVMKAQGI
                     KNFFIAKSLVAQSFIVGVVGVLLALLFAYLTSLILPSAMPFAVFWSQWLLYSGILIIV
                     AMLGGLFSIRTITKVDPITAIGG"
     gene            35383..36057
                     /locus_tag="SCJ86_000920"
     CDS             35383..36057
                     /locus_tag="SCJ86_000920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631710.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HEG1168501.1"
                     /translation="MIKLLVVEDDKVLSNNIKEILAAIGEITQVYDGSEGLFELESGV
                     YDLAILDLMMPEMNGYEVLAKIRKEKIQTPVLILTAKDSLEDKIEGFERGADDYLTKP
                     FYREELIMRVKALLKRSLGLFDENVLKYKDISCDLKSNEVLYKEQVLPIQGKEFELLV
                     YFLQNKGIILTKEQIFDRIWGFDSDTTITVIEVYMSNLRKHLRSTGLDKEIKTLRNVG
                     YILQRV"
     gene            36062..37402
                     /locus_tag="SCJ86_000921"
     CDS             36062..37402
                     /locus_tag="SCJ86_000921"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297809.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing histidine kinase"
                     /protein_id="HEG1168502.1"
                     /translation="MNGDITRKQRSRFFATNVAAFAFIFFLLGFITLQILNQSAYRET
                     DLSLEKINFDSRTVQLEIQRYQQGDPFLNPTQTEQNRTNMEQPLMEPSNDRFNTQVIL
                     WSKNGEILNKAAIGGRFNQIKELKMDTKNLNTIQNIELNEDSGSSALSFRSITKKAET
                     NQDGVAYIQILENTNQIKNSLATFRTIMILCMIVFWILSIGISYYLSSLSMRPIILSW
                     RRQKEFVENASHELRTPLTIIQNSLEHLFTKPNHTIIEESESIAQALSETRRLTGLTS
                     DLLTIARNDSDQQLLSKQMINTQEYIEQLVKPFQEMAIIDGKEFILHNDATLEVPIDE
                     KKIHQVLVILLDNALKYTNPEDKIILDSSVNHKEWLIHVKNTGPSISEEDQARIFDRF
                     YREDQSRAKETGGYGLGLAIAKQVIEQHHGQISVSNYLPQGVDFKVQLPLKPKK"
     gene            complement(37551..38537)
                     /locus_tag="SCJ86_000922"
     CDS             complement(37551..38537)
                     /locus_tag="SCJ86_000922"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337170.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2382 domain-containing protein"
                     /protein_id="HEG1168503.1"
                     /translation="MEEKNLTVIGSYSTREEALSVIERLRNEGYERDDIVIYTTDEAA
                     SRLGFDGLSGIDVETDENRMDGEEDRSLWEKIKDTFSFDTYDSETATPENDPLYQYRG
                     DISDGKFVITVKGYRQPETTEDTMDTQDTVSPASTMGTGLETGTGTDPMDVGGTTGFQ
                     NTTDPLNMTPGTDPMDVPGETDTMGTSKTEDIDRKPNLDDDTIQLKEEKLDVNTHDVT
                     TGEVDIHKHVVNDTETVEVPVKREEIVIERKPVTDQSSQGTDEKLEDDTITIPIKEEQ
                     VDVSKHTVIREEVGIHKEEHEDVEKVTEDVSREELDIDTSGDVHIEDRNKKS"
     gene            39261..40292
                     /locus_tag="SCJ86_000923"
     CDS             39261..40292
                     /locus_tag="SCJ86_000923"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298425.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HEG1168504.1"
                     /translation="MDSKTRKQLQEKLKQRTHLLPSVFQQFLEENRSSLSLGSRYEYA
                     KDFHLFTDYLRKVFSEEVDDQLIIHLEKQDYQNFLAKIYRHTRSFQTTANQETEQLFE
                     NSYYGISRKQYSLNHFLRFLYQKGLIEEEISLLGTSLPETTKAAPRYIDAELFKDFEH
                     LFIPIEGFQTSREASFEEKNRPRNLAITAILLYCGLKIHEVVELKRKDVSLTKQILNL
                     NRKENRLNKVPIPKEAMPFLENYFKLTAQNTDENSFVFRSSHDQQISPRTIRQILSKI
                     TVYKNVSPELCRKTYRYYAELYLDDADAVNYLCGNRYLSSHSFQDIWEKMVDFSYHEL
                     AAFVRVSSI"
     gene            40401..41933
                     /locus_tag="SCJ86_000924"
     CDS             40401..41933
                     /locus_tag="SCJ86_000924"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302023.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA synthetase"
                     /protein_id="HEG1168505.1"
                     /translation="MMETITYSPLNLFTNFQEAAANFSETPIIFDEPLSAFPELGLET
                     TYKKCCESVLKRAYQLAHLGVKSGDKVIIYKSSAFDTYLLAVSVSYLGAVPVMTSYHL
                     PTTTMEVFIDRLEDPFILFDDETKKRVREISNGTKSKQIPILYLMEQPALSVSQSFLD
                     KNEISYMTHTSGTTGIPKLICHSAHSMGWRTKWQKTVFTKISEKKLIAFHISPVHSRF
                     NIGISSLMSMGFPMMPLANAQSSKVVHMLEAHRPIALETHPNNFVQWRFTAKEHPEAF
                     SGIRYYHSTFDAINNQTMKTFLRTSMAQDAVFLQVYGQSECGPMILKAHTLESLKTSD
                     ARDMGVGLEDMTSARITDSVGNVLPENTDGHIQLLSKGRALTYYKEDARFQENVYGDW
                     WDSGDYGFMDKHGHLFLKDRQVDLIETINSTLAIEDFLLDSLDFLAEVVIVRDKNHSP
                     QPIIALAPDKEMDWNRWWEQVHELPRLNVPIIRDFDAIPRTATMKVQRLQIEKELKSQ
                     KS"
     gene            42072..42884
                     /locus_tag="SCJ86_000925"
     CDS             42072..42884
                     /locus_tag="SCJ86_000925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330524.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxymethylpyrimidine/phosphomethylpyrimidine
                     kinase"
                     /protein_id="HEG1168506.1"
                     /translation="MKRILTIGGSDPFAGGGIQTDLKTFENFGLFGLSALTSIGALDI
                     NERFMLESISLELLKRQLVSIDKMTSLDGIKIGLLNSSEAIMVVRDFIKTKQNIPIIL
                     DPVLAFKETEAVINQLYISQLIEELFPLVTMITPNLAEAALLSGQALPSSLTEMIHLA
                     KELTASGTKNIVIKGGTRMAGDEAVDLLYSIDHYEVFSHKKIDTTTVNGAGCAFSSAI
                     TANLVLGADFTKAVAESKKFVAHCISNGVLMNDHTGSVWSGGIQKGGMRNEN"
     gene            42874..43359
                     /locus_tag="SCJ86_000926"
     CDS             42874..43359
                     /locus_tag="SCJ86_000926"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286069.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ECF transporter S component"
                     /protein_id="HEG1168507.1"
                     /translation="MKTKQLTIYAMLIALTVALSLTILIPVPATNGFVTLCEAGIYTA
                     AFLYGPTGGLVVGASSGLLIDLISGYPQWAVFSLVIHGLQGYVAGSFSKSSTRMWGLG
                     LLLGSLVMIIGYGIAGWILYDWPAGVASIPGNIFQNLIGMGLAFPITASLQRIQARKQ
                     Y"
     gene            43382..43927
                     /locus_tag="SCJ86_000927"
     CDS             43382..43927
                     /locus_tag="SCJ86_000927"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290783.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR01440 family protein"
                     /protein_id="HEG1168508.1"
                     /translation="MKLSEKELKDQLTEIVNDILAEAHLKKGDLFVLGCSTSEVVGGH
                     IGKNSSAEVGQWIIRTLKELLDPKEIALAVQGCEHLNRALVVERTVAEAKNFEIVSVV
                     PALHAGGACSVAAFDQFNDPVEVEHVVGQAGIDIGDTSIGMHIKHVQVPVRPRLKTLG
                     QAHVTALRSRPKYIGGPRANY"
     gene            complement(44000..45643)
                     /locus_tag="SCJ86_000928"
     CDS             complement(44000..45643)
                     /locus_tag="SCJ86_000928"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-glucosidase"
                     /protein_id="HEG1168509.1"
                     /translation="MIQEQKWWKEAVGYQIYPASFKDSNGDGQGDINGIREKLNYLKD
                     LGIGFIWINPIYQSPFVDNGYDVSDYQDIEERFGTMEDFDLLLKEAHELGIKVIMDLV
                     INHSSDQHQWFEESKKSKESPYRDYYIWVDGVDGKEPNNWTSIFGGSAWEYSHETGQY
                     YLHVFAKEQPDLNWESEKLKEELFNMIRWWLDKGIDGFRLDAISHVKKDEYSVKATEN
                     PFSPFQNVSGIEEHLTDLKHVFEEYDIMTVGEASGVTAEEGPQWVGKDGYFDMIFEFD
                     HIHIWQQEKEGQLDVLKLKHALSAWQTSLDGIGWNALYMENHDVPRAVSVFGDTRPDF
                     WAMSAKAIAMMYFFLQGTPFIYQGQEIGMTNMPFESIDQVDAVDSKRLYKRLLAEGKT
                     REEALDIIRETTRDNSRTPMQWTSEQYAGFSTHEPWLITNPNTKTINVEQQEYEPESV
                     LQFYKNMIRIRQTNKGLIYGSYKEYLHEHPQLYVYERYLEDEQYLIMVNLTESLADYE
                     LPKEADQSWTLLLSNSSSGEFEAKGILAPYEARLYKTNK"
BASE COUNT        14082 a         8345 c         8627 g        14641 t
ORIGIN      
        1 taataaaaaa taggagaaaa agtagaaaag aaggtgtttg ttatttggga agtagttaaa
       61 tcgagagtac aagaaatgaa tgaacatttc tcagtgtcat tcgcagaaaa tccaagtaag
      121 tattttcgac gtcttgtttt tctgctcatc ttatcattgc tgttaacagt tgcaatgttt
      181 ttattgccgg aaaaaatccg agaagagcaa gtaaatagtc tcgtttattc gtccatacca
      241 accagcaaat tgaaaccttt tgattacagc aaagctgatc agaaaatcaa acaaagccgg
      301 gcgatatcgg tgatgttcag caaaccgaac gggaaaacct atcaggacat gcttgatgtt
      361 tttaatgatc ctgagcttat ggaagaattg aatcgaccga tcttttatta tccgatcgtt
      421 tatgatgtac atgaactgga acaaaaatat aacatacgaa cggatgaagt gacatttatt
      481 ttctttgatg gaggaaaaga agcaaatcgg ataactgcgg gtaagggagg aataactgat
      541 tttgataagg agttgatccc tgaactgaat cgacttccgt tagccaatat caaacaatta
      601 gaagaagaat tggatagaac gggagtaact tcccaaaatt ccgagattgc taattgagac
      661 attggcgcga tgaaacggtg ggtctatata gcgctatttt taattatcag aacaagtgat
      721 gtttgatctt caaaataaca aataaaaaat taaaaaatat agttgacgaa attcaaaaat
      781 tcgattagac ttaaatcaat tcttaaggaa agggtgacct gtaatgaact tatcaaaacg
      841 tatttcagca gaaaacaaaa aaaattggcg cagctggcgt agataagttg tattaatggt
      901 gagactttcc catagataca actttttaga gtgcatttct aaagcttgta tctatgctag
      961 acatgaacat tgactgccgc atagattaga atctatgcgg ttttttgtgt aaagacaatg
     1021 accgctttgg ccattgtctt tttttgtaga ctggatactt gcctgattat aataaataaa
     1081 aagattgact ttgcaggagg aaaaaaatga aaaacaaacg attaataaca gttgtagtaa
     1141 ttattttagt ttatttagca ggtgcattta tttatggaaa agcctcaggc gaaaaagaaa
     1201 gtacttcgac gaaccagaca aaaaaaacaa cagttgttgg ggtattgcaa tacgtgagcc
     1261 atccggcatt agatgagatt tatcgaggaa tcaaagatgg tctgaaagaa gaaggattaa
     1321 aagaaggaga aaatcttgag atcaaatttc aaaatggaca agccgatcag agcaagctag
     1381 ccacaatgag tcaacagcta gtacaatctg atccagatgc cttgattgga atcgcaacac
     1441 ctgccgctca atcattagca aacacgactt catcgatccc agttgtgtta ggtgctgtaa
     1501 cggatcctgt gggggctgga ttagtcgctt ctttagagaa acctggtggt aatgtaacag
     1561 gtgtttctga tcagccgcct gttacttcac aaatcaagct tgcttctgat ttgttgccaa
     1621 atgcaaaaaa agtcggaatc ttgtatgctt cttcagagga taattcaaaa taccaagtag
     1681 cacaagcaga agaagctgca aaaaaagaag ggttagaatc agtaaaatat gcggtgcctt
     1741 ccactaatga gattgcacaa acggttcaag tgatgagccg acaagtcgat tttatctatg
     1801 ttccattaga taataccatt gccaatgcga tgcaaacagt ggtaaaagaa gcaaacaaag
     1861 caaatatccc cgtcattcca tctgtggata caatggtgga acaaggtgga ctagcaacaa
     1921 tcgggatcaa tcaatatcaa ttagggcttc aaagcggtaa gatggcggca gaacttgcga
     1981 gtggaaaaga aaaaccggaa acgacgcctg tttatacatt tgatcaaggg gatacagtga
     2041 tcaatcaatc tcaagctgat cgcttaggaa tcattgttcc ccaatcgatg aaggaaaaag
     2101 ccaaaatcat tacagatgac tcgcagcaag aaacatctaa ggaggatgac aaatgatcgt
     2161 atcagctatt tcacaaggca tgctttgggc aatattagga ttagggattt tcatgactta
     2221 tcggatctta gactttcctg atatgacgac agaagggtcc ttcccattag gtgctgctgt
     2281 ctgtgtgaca gctattactc atggggtagc gccgattgta gctacattat taggcgtagg
     2341 tgctggcatg atagcaggac tcgttacggg gttactttat acaaaaggaa agataccggt
     2401 tattttagca gggattttag tgatgtcagg attgaactca gttattttgt ttgtgatgcg
     2461 aacaccaaat ctttctttat tgaatcatcc gattctacaa gacagcttcc atcgctttgg
     2521 gttacctgat tactttgaca cagtccttct tggcttcatc tctttgggca tcttgattgc
     2581 tgtactgcta ttcttcttca atacagattt gggtcaaggc tatattgcga caggagacaa
     2641 cgaaacgatg gctcggtcgt tagggataaa aaccgatcgt atgaaaattt taggtttaac
     2701 tttgtcgaac ggtgtcattg cattatccgg tgcgctgatt gcccagaacg acggatatgc
     2761 agatgtaaat aaagggattg gcgtaatcgt catcggtctt gcttccatca tcatcggtga
     2821 agtgatcttc caagaattga cgctagcaga aagattgatt gctattgttg tcggcagtat
     2881 catttatcag ctattgattt tagtagtaat caaactaggg tttgacacga cttatctgaa
     2941 gttgttctcc gctatcatct tagctatttg tctgatgatc ccgcaattga agaaagcact
     3001 gaacttaaaa acgggttttg agaaggaggc atagtaatga ctgatatatt gacaatcaaa
     3061 aatggcagaa aagtcgtaaa caatggaatg aatgaagaga aagtactatt ggatgaaatt
     3121 aaccttacaa tcagagaagg ggacttcatc actgtccttg gcgggaatgg tgctggaaaa
     3181 agcacactgt tcaatacgat cgctggtacg cttcaactca gtcaaggatc gatctctttt
     3241 aaagatcagc tgatcacaaa agattcagaa gaaaaaagag cacgattcct ttctcgagtt
     3301 ttccaagacc ccaagatggg aacggctcct cggatgacag tagcagaaaa tttgctactt
     3361 gcacaaaaaa gaggtcaaaa acggaaattc cgtttacgta acttgaaaaa tcaaaaagaa
     3421 gattttttca gactatgtca agaagtcggt aatggcctag agcaacactt ggatactcca
     3481 gcgggtgaat tatctggtgg tcaacgccaa gcattaagct tgctgatggc gacgatcaca
     3541 gctcctgagt tactattatt agatgaacat actgcagctt tagatcctaa aacagctaaa
     3601 gcattaatgc gattaacaaa tcagcggatc aatgaacaaa aattgacttg tctcatgatc
     3661 acgcatcgaa tggaagacgc cttgaaatac ggaaatcgtc tgatcgtact ccaaaaaggg
     3721 aaaatcgttc aagatatttc tgaaaaagaa aagcaacatt tgacaatggg cgaccttttg
     3781 cagttctttg aagaagtagt ggattgattt cgacagaaaa attcctttca gtagaaggac
     3841 ttgaaaactc attgacatct tcctttgatt catcctatac ttaaaacaat aaacaattaa
     3901 acatatttgg gtgttttgta ggatcacgta gtcaatgatt ttaacaatta aatagaatat
     3961 tctttgctct gacaagtaaa cgatgagaaa agaatttgct actttgccac ctagacgttt
     4021 cgtacttttt cgtatcaaac gctaagtggc tttttttatt gaagtaagaa caaaggagga
     4081 ataagatgat ttctggacaa accaaattag ctgggctctt tgctgatccg gcttctcaca
     4141 gtttatcccc attgatgcac aatactgctt ttgaagcaaa caacatcgat gcggtgtatt
     4201 tagctttcca agtaaatcca atgaatttga agcaagcagt cgaaagtatt cgaacttttg
     4261 atatgttagg tgtaaatctt tcgatgccga ataaaacagc ggttattcct tatctggatg
     4321 aggtaagcca ggaagcgcaa ttgatcggag cagtgaatac gatcgtgcac gaaaagggtc
     4381 gtttgattgg ctataatacc gatggtgcgg gcttcatgca atccgtgcaa gaagctggga
     4441 tttcgatcaa aaagaaaaaa atgactctac tgggagctgg aggtgcagca aaggcagtca
     4501 ctgttcaagc tgcgttagac ggcgctgagg aaatcgtagt ttataaacga aacaaccaaa
     4561 cattcgctca agtaaaagaa acattcgaac aactcagcta tcgaacttct tgtaagataa
     4621 aagttcgtga ctttgccgat acgcatcaat taagaaaaga cttgaaagaa agctgtcttc
     4681 tagtcaatgc aacagatatc gggatgggaa agaaaatcaa tcagtcaccg ctcttagata
     4741 taagcatgat gcatgagaat ctagcggttt ttgatttgat ttatgcacct agagaaacac
     4801 gtttattaaa agaagcaaaa aaagtaggag caaaaggata caatggactt ggcatgttga
     4861 tctaccaagg agcaatcgcg tttgaattat ggacaaagga aaagatgccg atccatgtga
     4921 ttgaaaagct gtttgattaa aaaaggataa tagataaaga tcttttattc tcgcataaac
     4981 ggtctcaaga aaagtaatcc tttatgtatc gaagcattta tttcgttatt ttttattaaa
     5041 ttatcaaaaa atgataggct tttgtcgatt ggttatcgta tcctttattt atcagtaaga
     5101 aacgaggagg aaccacaatg agtcggatgg atcgctataa ggacatccat aagaaggcga
     5161 aacctctgaa gcgggatacg gggagtattt ttgctagaag agaacgaaaa cgaacgcgta
     5221 tggaaaatga ggaagatgct acccgtattt atgaaagaga gcagcattca tttgaacatt
     5281 tgaaccaata cgagcaacag atatacagag atgcaccaag agcagaagaa caacaaaaaa
     5341 ataaaaaatt tttcaatcgg caaaagaaga acaaaccgtt aaaacctcga agaaagcgat
     5401 cgtggaaaaa aatcattcta tcgattttgc ttgttttgat cctttattca ggtatctcat
     5461 tttttattgg taaatatgta gctgaacatg acacaagctt agaggcagcg acttcagaaa
     5521 cctttcatgg gcaaaaagcg acgaatggtg cgcataatat tttgatcctt ggaagtgata
     5581 cacgcggaga agatgccggg cgagccgata cgatcatggt cttacagttg gatggtcctt
     5641 cccataagcc gaaactgatt tcttttatgc gtgacagctt cgttacgatc ccaggggtag
     5701 gacaaaataa gatcaattca gcttatgcat acggaggagc cgatctagtg cgtcaaactt
     5761 tagtagaaaa ttttggaatc gattgtcaat actatgcgaa agtcgatttc aaatcgtttg
     5821 aaaaagtgat cgatgcctta ttcatgaacg gagtgaagat cgatgcagaa aaagatctga
     5881 atctggatgg tgtagatatt aaaaaagggg tccagaagat ggatggacat gttcttttgc
     5941 agtacgcacg tttccgaatg gatgaacaag gagactttgg ccgtgtcaga cgccaacagc
     6001 aagtaatgaa cgctattttc agccaattga agaatccatt gaatctgatc cgtgcacctt
     6061 atgcagctgg taaagcttta ggatatacat ccacagatgt cactgcttgg ttcttgacga
     6121 aaaacctctt ttcgattgca cgaggagctg gtggcattga tcgaataagt gtgccagtag
     6181 acggttcgtg gaattatggg caaagtgatt atgctggcag tattcttgaa attgataatc
     6241 aaatgaatca atctgctatt acagattttt tgaataaatg atcataaaaa agatcaagaa
     6301 gaagctgcgc aaggcttttg tcatgaatca atcaaacgat tcactcgtaa aaaacgcttt
     6361 cttatggtag atttaaccaa gagaggagct gtttacattg gatatctatc tgaacttcaa
     6421 aaatgagtcg cgtgaagcca tcgcttttta tgaaaaagtt tttcagactg aagcatcagg
     6481 tatcaccaca tacggtgaaa tgcctggaat agaacaaatg aacttgccag aagaagcaaa
     6541 aaatctaatc acgaatgcac ggctgaacat ttatgataca ttagttatgt ttgcagatac
     6601 gccagaagga attggtcctg aattagttgt gggaaataat gtttctttag tcatccagac
     6661 aagaaatgaa gaagaaacga agcgcctatt tgaaagctta gctgagggtg gtaagattga
     6721 gatgccactg gatgctgttc cgtgggctca gttgtatggg atggtaactg atcgatttgg
     6781 catcaactgg cagattaacc aagagtgaca gctaaagatg aatattttct ggaaacatca
     6841 agcagcaaac ggatcatcgt ttgtttgctt gatgtttttg tgtctatttc gactatacgg
     6901 tatctttttt ttattgccag atgcgaaaat atgttccata atagagaagt ccgcaaaaaa
     6961 ttaagtaacg gaggtcagaa gatgaagacg gtgatactag ctgaaaaacc ttcacaagca
     7021 aaagcctatg ccgaaacctt ccaacagcaa aagcggcatg aagggtatta tgaaatcagc
     7081 gatccattgt ttgctggtga agtaaccatc acttatggtt ttggacattt ggtagatatg
     7141 gttgctccag gagcttatga agagcgttgg gcaaaatggt ctttagaaaa tttgcctatt
     7201 ttcccagaaa ccttccgtta cgaagttcca atggacaaac gtgcacaatt ttcgatcgta
     7261 aaacgagaat tgcagtcagc cgataccatc attattgcaa cagatggtga tcgagaaggt
     7321 gaagccatcg cgtggtcgat tattcgacaa gcaaacgcat tttctaagga aaagaactat
     7381 ttacgtttat ggatcaattc tcttgaaaaa gaagcaatct atgaaggttt taagaatctc
     7441 caacctggaa agaactattt tccaaaatat aaagaagcgc aagcaaggca gaacgctgat
     7501 tggctgattg gaatgaatgg aagtccatta tatagcttgc tacttcaacg aaaagggatt
     7561 gatggcagtt tttctcttgg tcgtgtacaa acaccgacac tctatatgat ttatcagctg
     7621 caagaagaaa tcaaacactt caaaaaagaa acgtactttg aaggagaagg tttaattacg
     7681 acaaacaaag ggcagttttc tggaaaaatc gtaccaaaaa aagcatttaa aacccaagaa
     7741 gagttaacga aggaaataga aaaacttgga gcgcatttag gtaagcaaaa cggacggatt
     7801 cttgaagtta cgaaaaagga aaagaggttg catagtccac gtttattttc gctatctagt
     7861 ttgcagacaa agatgaatca gctaatgaaa gcaagtgcca aagctacact tgatgctgct
     7921 caaggattgt acgaaagcaa gtttctaagc tatccaagaa cggattcctt ttacattaca
     7981 gaaaatgagc atcagtatct ggttaaacat ctgcaaaatt ataaaaaatt tttaggaata
     8041 gaagaagtac agacaactga gatcaaagca aagaaacgat atgtcgatgc gaaaaaagtt
     8101 caagaacatc atgcaattat tccgacaaag acgattccga ctcctgcacg ctttgcgaag
     8161 ctttccaaat tacagcaagc aatctatttg caagttttgc gcactactgt ggctatgttt
     8221 gctgcagatt atatctacga agagacgacc gtaatgacgg gcgtacagca attaaagcta
     8281 aaaagcatcg gaaagatccc attacagcaa ggttggcagg aaatcttacg catcaagtca
     8341 aaaaagaaag aagctcagac tcttcctcct atcgtagaaa atgaagaagc agaagtagaa
     8401 ctgaaaagta tggagaaaga aacacaacct ccaaaaccct ataccgaagg aacgttgatc
     8461 acagcaatga aaactgcggg aaaaacgctg gataatgatg aagcgcaaga aattctcaaa
     8521 gaagtagaag gaatagggac agaagctaca cgagccaata tcattgaaaa tcttaagcag
     8581 aagcattaca tcgaagtcaa taaaaacgaa ataactgtta cgccaaaagg aatcactctt
     8641 tgtaaggcag tagccaatga accattactg acgagcgcag aaatgactgc gcgctgggaa
     8701 gggtacttga gaaaaattgg taaacaagag gggacaccat ctgttttttt agagaatatc
     8761 aaaaaattca tcctacattt attggcagaa gttcctaatc aaatcgacaa ggtagacttt
     8821 tcagatgaga ttagcgggac acgtcagata aaagcgatgg agaagaaaaa cgatcaatta
     8881 ggaagatgtc caaaatgtaa aaagggaata gtcatgcttt atcctaaaat cgctacatgc
     8941 ttgaatccgg aatgcgactt caaattgtgg ccaacagtag ctaaaaagaa gctgactaaa
     9001 acgaatctca gagatttgct ttcaaaagga aagacaagta aagttgtcaa aggttttact
     9061 ggtaaaaaag ggaaattcga tgcagtttta gtattaaaag aagatatgac aatcggtttt
     9121 tcttttccat ggatcgaaaa cagtggaaca aaagcagttg atggaaacaa agaaacatca
     9181 agaaaaaata atgaaaacga agtagagtaa atgaaaacaa tgaaatagcg gaatatttat
     9241 tccgaaaagt gagaatataa ataccaataa aactaaaaaa tatcagtgaa tcaagcaaaa
     9301 acgttggggt aggctgattt tatatagata gcaaggagtt tttttatttg aaaaaagtgc
     9361 attcattttt aaattatctt atgggagcaa tcacgattgt ggctgtgtac caagcatttc
     9421 taaagtatcc tgattacggg acgaagttgt tgtcattgac gcacaatcaa acagcatatt
     9481 caccaacgtt gattttatcg actttgactg caggagctaa aagcgctact tatttttttc
     9541 ataaaacgac aagcatgcta aagcgaaaga aacagcgaaa acaattgttg gtggtggtag
     9601 cggctatgat cattatttat tttctaccag ttattacaca agtatttcat caatattttg
     9661 gttaagaata cgagagtttg tgacaaaagt catgtcacaa acttttttct ttgtcatttt
     9721 ttcgtaagaa aaagcaaaat aaaagtatat acattgatta aaagtatata cttggatata
     9781 atgtacttac tatagaatga gaatggtata gagggtggcg aaatatgaaa aaaatgaaaa
     9841 agtataaaat tagaaaatct ggcaatagcg atgtaacaac tatcccacca gaagtaaaag
     9901 aattcatggg tgtgcagaca ggtgacgcaa tcagttatgt ttttcaatct gatggttctg
     9961 tacgaatgat caaagcacaa gaagaacctg atattgattc attagttgat tcgatcatga
    10021 atcaatatga agacgcgtta aaagatttgg ttgatttatg aggtatcttt cgactaaaga
    10081 aattatcaaa atcaacgcga aagttatttt acgtcactcg ccaggcgaaa tgatcggtgt
    10141 aaaagatgca aatgcattag atatggccgt caaacagcct tcacaagcag tattcaatca
    10201 agaactctat cctgaaatct atgaaaaagc ggcgatttta gcaattaatt tagctaaaaa
    10261 acatcctttt cataatggca ataaacgaac tgctcttgtt gccatgttgt tgtttcttca
    10321 attgaataat tgtccagttg ctttttctcg tcaagaagct gttgatttta tcataatgat
    10381 taccactagt tcattggatt tcgattcact aaagtccaag ataaccagtt atttacgaca
    10441 aaccgcaata aaatgaacga attaaaaact ttatttctca attagatcag catgtcatta
    10501 aggaatatgc tgattttttt atgtttaatc atacaaaata gttttttcat aaaaatatgg
    10561 aaaatatgga ttaattttga aataattgat ttaaaaggaa tacaaaagag tgaatagtag
    10621 gaaatagtgg tttagaacta gtgggctgat tttttatata ttttagttta cataatgtac
    10681 attatacaaa gtagatttaa aaaatgataa acctctctcg aacggttttt tatcaaaaat
    10741 cgtcattttt ccatcaaaat ctgcttatgc aaaattataa aaaaattttg cagtttttct
    10801 tacttttttt gtcgtttaat tgaatgataa aaaccagttt ctttattcag ttaatcaaat
    10861 atgaacgaat tagatatgaa tcattgtatt tgccgttgcg gaaggatttt ttaattaacc
    10921 acagataaac attgataacg attcgcttat tgtaatcaac agattaaatt tcatatgttc
    10981 atatgtacgg atacattttc ggaccgatta attttaacca ttctgtttat ttgcttacct
    11041 attagatatc aataaaaaac tgcttaacag ctatttcagc tgttaagcag ttttaggatt
    11101 tttagttcat attcattgtt tcttataata cagtgttcaa tacttctctt aaagcacttg
    11161 ttgatttgct gaacattgct ttttcttctt cgttgattga taattcaaca acttcacgaa
    11221 caccattttc gtcaacgatt gatggcacac ctgtaaagat atctttttcg tcgtattcac
    11281 cagttaaata agcagataca ggcaatacag cttgttcgtt attcaagata gcttttacga
    11341 tacgtgcagt actcatacca ataccatagt aagttgcttt tttacgatca atgatctcat
    11401 aagcagcatt acggacttta tcagcaatca catctaattc atcttgggca atacgatggt
    11461 ctttttctac aatttcaaac actggtttac caccgacagt tgtatgtgac catgcagcaa
    11521 cttctgaatc accatgttca cctaagatat agccgtggac gctgcgtggg tcaactttta
    11581 atttcaatgc gatttcttta cggaaacgag ttgtatccaa tgttgttcca gtaccgataa
    11641 cacgtgatgt tggcagacca gattcgttcc aagcgatata tgtcaaaata tctactgggt
    11701 ttgaagcaac aacgataata ccgtcgaatc ctgatcccat gatttctttt acgatttggc
    11761 gcatgattga cgcatttgtt ttaactaaat ctaaacgaga ttgaccaggt ttttggttga
    11821 taccagctgt gataacaacg atatcagcat ctttacattc ttcatagcca cctgaccata
    11881 cagccacatt ttcatcgccc caagccatac catctaaaag gtccaatgct tccccttctg
    11941 ctttttcttg attcacatcg atcaatacta attcattaga gattccttgg ttgatcattg
    12001 cataagcgat acttgttcca acaaaacctg taccgacgat tactacttta cgacttgttt
    12061 ttttcatgaa taaacagctc cttttcataa ctatttgtta ctttcatgaa aagtatagtc
    12121 ttgtttattc attttatcaa catgttaatt ataatagttg ttattataat ttttgatgga
    12181 aacgaattcc tgaatacgca taacagataa atgacaaatg tttacaccta tatcccctga
    12241 taatacaacc aaaagtttag aactgaaaat aaatccaaaa aaattcgctg caacgatata
    12301 ataagacagc aatcgcacct aaaacaattt tccttcatat gctttagtga aagattttct
    12361 tcttaatgtt tatttctcca ttcattggga gtacatgttg aatacttctt gaataatttg
    12421 aataaagaag tcgtttgttt atatccgata cgattagcga tttcttccaa cgaaaaactc
    12481 gtttcttgaa tcagttccaa cgcaaagtcg aattttttct ttagaaccag ttcttgaaag
    12541 gaagcatcaa aatacatttt caatttatta ctaagataga ccggattgta gccaaaatga
    12601 gctgctgttt cttttaacgt acaagaagta aaatgcttat cgatataatc taatactcta
    12661 agcatctcat tttctgcgca tccatctgct gatgaaaagc tttgttgttc taataaatga
    12721 gtcaattcga tcagtaatag tgatgtatag agatttaacg aacggatctt atgactgtat
    12781 gtatcaaaat aaatcaacag catattttca atcaaatgcg tcaaacgact tgtttttccg
    12841 ccgcgaaaat gaatgaattg tgtatgttcc cctttttctt ttgatgcatt tatcaaaaaa
    12901 gaagaaagta gattatttgt gcttgccaaa tgattcaata tctctgtaga taaggtttct
    12961 tcttttaata agatgttgat taaaatatcc tcttgccctg tgtattcgat tctttgagta
    13021 gcttctttat caatgatcac cacgtcactt tcttccatct ctatcctttc tccattaata
    13081 atttgtaaac atgtaccgct atacatataa ttgatttcaa caaaatcatg tgtatgctcg
    13141 ggaacttccg catatcgacg gtgtttactg atatgaatat ttccctgttg aaatagatcc
    13201 cctcggtgga tttttggcgg tgtctgaaga tggtaagcta agtttaaatc attgatattc
    13261 attccttgtt cacgctgcag agtttcgatt gattcttcgt tacgcaaaaa aatgtctaat
    13321 tcagctagtt ccaattgact ccctcctttt tgtaagcggt ttctttcgct tattagttca
    13381 cgctgtactg ctattatatg caaagctatt tttggtttca taaagacttt ctatggttgg
    13441 gacatgaact tcctgtgatt ccgctattga gagaataacc acttcatact atatttttga
    13501 taagcgctta caaaaatgaa aggaacatgc ttatgaataa aaagatgatg aaattttctc
    13561 tgctgtcagt ctctcttatt ttgacttctg ctggtgcagt cacgtctgca attcccagta
    13621 tgtcagctac ttatgccacg cgttctttat ctgctgttga attattaacg actgctccct
    13681 cttttatggt cactattttt gttttgatta gtagttttat tgctaggaaa atcggacaaa
    13741 aacaaactgt gatcatcgga ttagtgattg catcgatttg tggtatcctc cccctcttct
    13801 cacaaagtta cagtattgtc ttagcttcaa gagctggact aggtattggc tttggattga
    13861 tcaattcttt ggcagtcagc atgattgctg ccttttatca cggagatgaa cgagcctcta
    13921 tgattggttt tcaaagtgca tttcaaggat ttggtgcagc tttaatgaca tttgttgcag
    13981 gacagctagt acagttcggt tggcaatata cctttttagt ctacgccatc agcttaccta
    14041 ttcttgtctt gtttgttttg tttgttccta cacctgaaac aactgaactt caatcgcatg
    14101 gcacagcccc aacaagacaa gtaatcaaaa cgaatcctaa aatgattagt tatggtattt
    14161 ttctcttgct ggtcatcgtg gtatataatg ctgtatcgat taaattagcc acagtcctga
    14221 cgacttattc tctaggaaac gaaactaatg cagcgaatat cttaacaatc atgcaattgg
    14281 caagtatggc tgccggagtt tgttttggct ggcttcagac aaaagcacat cgctatatgc
    14341 ttacactttc tttactactg atgggaatcg gctttgtatt gattgccact gtgataaacc
    14401 tgtatgtgat tgggtttggt gcgatcctaa caggaatttc tttttcgtta ttcattcctt
    14461 acttattcaa ccaagtaagt atccaagcgc ccaagtacgc ggagagtttc aatacttcaa
    14521 tcttattggt tggcgctaat ttaggtactt tcattgcacc atatggtttg ctctttctct
    14581 cttttgcttc tcttgggagt aaaacacttg ctgttttcgt caatggcagt atcttgctgt
    14641 tgattagcgc gcttattagc cttctagtag tacacaggac gaatagaaag acagtgataa
    14701 gagtagatga agaaataaaa gaacaattga caacaaaata aatttgatgg aggtcgtgta
    14761 aaatgacaaa tgaaaaacat aaagatagat ggctttggta tcctggagac tttgagatcc
    14821 gccatggact gctacaaaat ttccaacggg aagaacgagg ttttgactgg ccagcgtact
    14881 ggtacatgga tgattgccat cggaacgtca agtttaagag atattacttt cttgatcaac
    14941 cttccatgtt taaagtgacg atccaaggag ttgggtatgt agaaattaat ggacaaaaac
    15001 atccatgtgg aaaatggctg acttgtccag ctggtaaagc aaaaattcgt atctttgtag
    15061 gacatacatc agggctacca gcgatgttta tcgagggaga cgaagtaaaa tcagatattg
    15121 gctggacagc aagtaatttt attgaagaat atccagctgg ctggagtccg ctttatgtcg
    15181 atatcgcaaa agatccaaat caaatctatt atcaaaaaga atgcgtccat ccagtcactg
    15241 aaacggagat tggcggagga atcttattcg attttgggcg agcagtgaat ggctgtgtag
    15301 aactaatatc tctggatgac catatcgaat ctatcactct atgttatgga gagtccgatg
    15361 tagaagctat ggatatcgat tattgctact ataagcaaat aggaatgaca agaaaaatga
    15421 aagctcgtaa acgcgctttt cgctatttgt ttataccaga ggtttctact ggaatggttc
    15481 aacttcaagt ttttcatgaa tttttatctc tttcgaaaca agctgctttt tcaagcgatt
    15541 cttctttact gaatcagatc tggtcagtat cagaagaagc cttcaagtta tgtagtgatt
    15601 tgttttttat cgacggtatc aaacgagatc gctggatttg gtctggagat gcgtatcaat
    15661 cattcatgat caatcaatat cttttttttg acgaggaaat caataaacga acgatattag
    15721 cattaagagg acaaaatgaa atcaagcagc atatgaatac tatcgttgac tattcgatct
    15781 tgtggttgat tggtatcgaa aaccactata tgatgacgaa agactatgaa tttttacgca
    15841 tcgtctaccc taagatgcaa tcattaatga gatacttgat ggagcaaacg aatgaattag
    15901 ggtttattta tgggagagca aaagattgga tatttattga ttggtctgaa atggataaag
    15961 aaggaacgct tgctgcagaa caagtcttat tactgaaagc ctatcattcc atgatcactt
    16021 gcggagatat cttagctgaa acatatggag agaacgaaaa ttatcatact catctctatc
    16081 aagaaaaata tcaactattg aaacaaaacc tatttgacta cttttgggat gaagaacttg
    16141 gcgcttttat cgattgctat gaatctggga gaagaaatgt gacgcgtcat gcgaatattt
    16201 tcgctgtttt attcgatttt gctaacaaag accaacagca atcgatctta aagaatgtct
    16261 tattgaatga cactatcaca cagattacta ctccatattt caaattctat gaacaagatg
    16321 ctttatgcaa actcggacag acgaatatcg tctaccaaac gattcttgat tattggggag
    16381 ggatgttatc tcatgatgcg gtaactttct gggaagaata cgaccctact caaacaggaa
    16441 atgaaaagta cagtatgtat ggagatcctt atggaaaaag cttatgccat gcatggggag
    16501 caagcccaat ctatctttta ggacgctatt ttatcggttt gtatccaaca aagccaggtt
    16561 atgaagaatt tatgatacaa cctaatctag attcatttga aacactgaat tgtacgctac
    16621 ctatcaaaga tggaaagatt tcgcttgaaa aaacaaaaga taagatcgtg attacttctg
    16681 atagaacagg cggaacagtg aaaatgaatg gtaaaagtta tcctcttcta gcaaaacagc
    16741 cacttgtctt aacaagctat tcaaaaataa atgcataaaa caaaaaactg cttgcgacat
    16801 atcagaagac tttaaatctt cttctgatat tgtccaagca atttttattt tgaagtttgc
    16861 ttaattctgt tctttagaaa caatgtttgt tctttttccg gcaaaaagaa agctagataa
    16921 tacgacaagt aaaaagaaag cgatgattcc aactgtcaag gctatttgac cgtattctcc
    16981 tagtttccca agtacaagtc ctggtactag ataatcagta tcggcaaatg tagaagaagc
    17041 gattttaaga tctcctaata ctggaaggat aaataatggc agccaactaa tcaataagct
    17101 attgaaagca gaacctaata ccgcccctct tctaccgcca gtcgagttgc cgaagacacc
    17161 agaagcagct ccgcagaaga aatgtcctac taccccagga atgataactg tagtttgcaa
    17221 gcctatcata gcaaacatgg atagtgttcc tacaaagaag ctaacaaaaa agccaattaa
    17281 tacagcatta ggtgcatatg gaaaaacgat aggaacatct aatgctggtt tagaatcggg
    17341 tacgagtttt ttagcaatcc cttggaatgc tggaacgatt tctgccaaca ccattcgaac
    17401 cccttgaagg acgataacaa acccagctgt gaatgttcca gcttggatca gtgcaaagat
    17461 tacagcgttg tctccattgc ttagttctct ttcaacaaat ccaggaccag ctaacaaagc
    17521 aagtgcgaca tatacgatcg acatcaagag catgatcgat acagtagaat ctcgcaaaaa
    17581 gctgaaactt ttaggaatct ttatgtcctc tgtagaacgg tctttgtttc caaaaaactt
    17641 tccgatccat ccactagaag cataaccgat atttccggta tgccctaacg caacttgatt
    17701 gtttccagtg atgttcttca taaacggttg acaaagagca ggcgtaatag ttaatagtgt
    17761 tccttcgaag accccgccta ataagatcat ccacaaattt gcagtaaacc ccgcgcttac
    17821 taaaatgact gctgttagac aagaaacata aagcattgct tgacctgtaa gaaaaatgaa
    17881 tttatatctt gtgaatctag caagtaaaat attcacgatc atccctacta gcatgatcaa
    17941 agcagtaggt gtaccgtatt gttgcaaagc aatcgccact actgcttcat tgctaggaac
    18001 tactccttga gtatgcagag ctgtttggaa catcgaagca aaaggggtta gtgaattact
    18061 gattaaacca gcaccgccga tcagtactag gaatccgaca aatgttttga tagttccttg
    18121 gatcacttct gaaaatttct tcttctgaag aacgagacca agcagtgcaa tcaaagaaac
    18181 gaggatggag ggctgactga cgattgacac aaacaaattc aacaatttat ccattgatag
    18241 ttcctccctt gaatcatttt tattcttttg acaagatctt cgttactttt tcttttactt
    18301 cttcgctgtc aatgatcgat tgcaaaggta cgagcttttc atcaggtata gaagttagcg
    18361 tatctttcaa agaatgctca atgaagaaat aatcagccat atcgctagat acacttccaa
    18421 catccgtatg acttacttca aactcgtcca catctgctcc aagatctgca agtgcttgtt
    18481 tgatgttcat ctctaccata aaacttgttc ctaatccact ttgacatact gcaaaaattt
    18541 tcatatcatt cgtctccttt tcttatgatc gctaatattt cttcttttgt tgatgcgtgt
    18601 aataaatctt gaagattttg tttattagaa agtagctttg ttaaatttgc caaagcccga
    18661 agatgtgcct cattatcgat agcagctaaa cagatgaaca aactaatggg atgggctgga
    18721 ttatcaagca gatccacagg ttgttgaagt ttcagcaaag acatccctac ttcgtttacg
    18781 ccatcttctg gacgagcatg aggtaatgcg atgtgatcgc caatatggat aaacgcacca
    18841 tactgctcca cgcgctcgat catagctttg atataacttt cttctatttt attttctgat
    18901 aatagcggct gtgcggcaaa tgtgattgct tcttgccagt tttttggttg atccgataat
    18961 tggatcattt cttctgttaa taattcagat agcataggac gttgatcctc cttcttttct
    19021 aaaagtttgt tcattttacg atttaatact tgatagagct tttcttcgtc tacgccttct
    19081 tttaattcga tataaggcag tagcgccgac aacaattctt gggcagaagg cattgaaaat
    19141 cctggtatca gccaatctct ttgtacttga ttgatcagct gatttttttc caagtcagtc
    19201 atgattggat gcagaacata gagagacttt ttcttatcag taagtgtgag aggtacagta
    19261 gaaaaaataa tatcgtaaga agtttcgtca acctcgtgta agcggtcagc tgaattagtc
    19321 tctgtaaaaa agatcatcgg gaataattgc tgaagttcac tttttaagat caatgaagag
    19381 ctaatgccac ttggacagac gatcacggct cttatttttc tatttctttc ttctgcgtcc
    19441 gcttttctga tctctccgcc aaatagtata gtgaaaaaac caatctcttc ttcagggatg
    19501 ctcttcccta ttcgtctttc taacggaagt aatgcttttc ttgtcatttc gtagatggaa
    19561 gcatattgat gcttaatttg atcgatcatc acattaggta aataaaaaga ataggaaata
    19621 cgaaaatagg cagggaccaa gtgataaaag acattcatca ataattcacg aaacgaagca
    19681 aattctacgg ccatcaggaa ttccatgcga tggatgactt ctgaagaaca ttccagcaaa
    19741 aaatcaagtg caggctcctg gacatctcct tcaacgatcg atgctaagca aagagagaaa
    19801 aatataatct ccgaattttg atgttcggaa tagccaaggc cagtcaatac ttgtcgacta
    19861 atgtcagtga ctgcaagaga agccaacagt tcagcatctt ttaccagttc catggatgtt
    19921 tttccaattc gaatggttgt caaactcaaa taataagcta attcatccat tcgatttggt
    19981 gcgaatcgaa gatctgatag tgaaatcgtt ttatctagca aatctctaac tttggcgtaa
    20041 taatcaatgg aaagggttgc ggttaaatac gtgagcccaa atcttccagc aagtgactgc
    20101 atcaaacgag cagtgcgatt ttttgcttct cttcttaata ttgcttctga ccctagcagc
    20161 ctaaaaccag ttttcctagt atacgacagc tgacaatctt ccacttctaa ttcatttctt
    20221 atctttttga tatctgacag gactgtccct ttagaaacct gaagaaaatc ttgaaaatga
    20281 aaaacagaaa cagacgttgc agtcgaataa accatcaaat aaagaagatc tcttcgctct
    20341 tgttcggtat acacagcttc aaatcgagta atatccaaaa atttttctgc ccagtcgatt
    20401 gctcggaatt tctttatacg gatacgggta tcatccacat attcggcaaa aggagttaat
    20461 tgtttgatcg cctcgcttgt atttacttcg atgatctctg ctgctttttc tagactgatt
    20521 tctcttggtg ttttcaggag ataactaatt ttgtagttca cagcgctgtt cacctcctac
    20581 ttatattcta gtatggtagc gcttttaaat cgaggggtgt ttggtacaac ttacagttgg
    20641 atttttttta cgactgtacc gaacgatttc tatcgaatgg atacagaaaa aaagcaggtg
    20701 accaaatgtc tcctgctttt atgaattgat ttcgtccgat caatgatgat catgtttatt
    20761 ttttgaggtc agttcttcaa atttttcttt gacatcttca accgcttttt cgatatcttt
    20821 ttcaaagtga ttgggctcat taggctttgt ttcctcttct acagaatttg aaccagataa
    20881 agctgtttct ttattttctg tttcaccaga gtttgtgcca gctaatgatt cttctttgtc
    20941 ttctgtttca ccagaattag ttcctgctaa agccacttct tcttctgttt taggggccat
    21001 tgaacctctt aatcgtttgt gtttttcacc gtcataattt gtcatgttgg ttcctcctag
    21061 tagttctata ctttttcatc gtacaataag acagtttatg cgtcaaaaaa caaacccgat
    21121 tcttacaaat atgtcatatt tttccttctg attcttgaat gcttttgatt ccttcttcca
    21181 aaattttcat ttgtaagatt gtttcttcat ctttgatgac tttttctgcg ccattaacta
    21241 atgtctcgta aatcccttcg tagacacggc tgtaatcacc aacctcggaa attacttttt
    21301 cttcatgata actcccttgt tcatctatat aggtcaatac accataatgt tcgggcaaat
    21361 cgataccaaa gtcttcatga tcaggcatgt aaaacatttt cagatgttct tcttgtcgat
    21421 cttttgtttg tttcacgaac atcccttttt ttccgtaaac gacgaaactt ggacgttctt
    21481 tgatgcggaa ataactggat tttatcgaaa ttttcatatt tccatagaaa aaatccagat
    21541 cgaagtaatc attcattcgt cctttcccta aaagttgacg tacatcatag ctgatatcat
    21601 ctggttcgcc gaaataagaa agaacttgat ctactgtatg acaagcatgt ccatataaat
    21661 agctgttcgc agtagaaaat tcatgcgtat tttctggtac ttccggtctg aaataatcat
    21721 aatgcatttc tacttctaaa atatccccta attttccgct ttcgattact ttttggactg
    21781 tcaagaagtc agaatcgaac cgtctgtttt ggtaacattg gataaataga cctttttctt
    21841 ttgctaatgc aaataactct tctgcttcct ttgacgtttc tgcaaaaggt ttctctacta
    21901 gtacattctt accatgttcg agtgccattt ttccatattc ataatgaaat tgactaggtg
    21961 tagtgatgac aactaattgg atctcaggat catcccaaag atcagacagc tgatcagtat
    22021 attcgatgtc ttcgaatgtt ttccacactg gttcagatgg ggaaaaaatt tttttgatat
    22081 tgattttgtt cgtaagtttt cttgaaaaag gcaaatgata gcgatttgta ctctttccat
    22141 tgccaagata agcgattgta agcatgagtg gtgcctcctt ttattgatgt tttcagtata
    22201 tcctagaaaa gcgcagaaag tatttataat agctattctg gtaaaaaagt tcagaaaaac
    22261 acttgttatc attcggtttg gtgaaattaa tgttcaaagt aaggataaaa atcaaaaaga
    22321 tagtagcgga accaagtgtt tatcagtaag atagaatttg gaggtcagat catgcttatt
    22381 gaagaaaaac taacaaaaca agagttgttt accacgacag agaaacggat tgtcgattac
    22441 atccgtagga atatcgaagc tgcagtctac atgacgattg aagaattagc aaaagctacg
    22501 tatacttccc attcagctat cattcgttta tgcaaaaaaa tgggattcag cggatttaaa
    22561 gaattccgtt tcgagctggg gcgggaagtt catcaattga ttgctcagtt caatcaaacg
    22621 attgatccta attttccttt ttcaagtgaa gatagcactc agcaaattgc aaagaaaatg
    22681 gcggatttat cgatccagtc aataaaaaaa gcacaattgc agattgaaca tgaagattta
    22741 gatcaaatcg gtaaagtatt gaacaaggca cagcgaatct ttctttttgc aaaaggtgat
    22801 tctcaaatca cggcaaggaa attccagaat aaaatggtca agttgaataa gtttttgatc
    22861 atggctgaag aatacagtga ttcttcttgg aatgcagcca atttgactag cgaggattgc
    22921 gccattttta ttagctacag cggtcgcatc catcattatg aacgaataat gacctatttg
    22981 aagcatgtag aggcccctac cttgctgatt accggaaatc agcattcaga aatggccaaa
    23041 caggctgata tgtgtcttgt tatatcgcag gaggaatatg attttgctaa agtagcgact
    23101 ttctcctctc aaattgcctt cgattacgta ttaaatacgt tattctcagt gatatattct
    23161 caaaattttg aagagaatat ccgcaacttg aaaaacaaac aagaattgat gcaaaaaggc
    23221 cttcttaaag aatatcggaa aacttaagaa aagatcctca tttagtaata agaaagatag
    23281 tgtttgatag gctctctttg atatcatcat aaaaacaggc tgaagttttc tttttcaaac
    23341 ctcagcctgt ttgtttacgg aacgattcga atagatatat tatagaaatg aggaatagct
    23401 aacttctgtt tcttattttg aacgattgac gaaaaagata tataagaagg aggaaaatca
    23461 gtatacatgc agctaacaat ccatacagga aaaatcgttt gttctcggat actttctgta
    23521 cttctttttg aacttctttt gcatgataag gaacccggtg ccctctgaca agtaagcgat
    23581 gactgttgac catataaggt gtacaagtta ggagtgtcac gaaatcctgc cctttttcga
    23641 ttctcagatc ttttgtatca gtcggttcta ccgtcttgat ctgatcgact tgataagcga
    23701 gtgtccgtcc attgatttcg atatagaaag aatcacctaa agtcagtttc ggtaagtctg
    23761 tgaacaactt tgcttgaggc agtcctcgat ggctggaaat aacggcatgt gtattttctc
    23821 cccccgtagg ataagacgtt ccttccaata atgaggcacc tttttctagc agcacagtat
    23881 tcgtttcatc aaaaattggc aaatggacat tgatctcagg gatcgtcaaa ataccgatcg
    23941 tatgtttctc aaaataggtt ctatcctttt tcgcaggttc ttttttctta gtaaagggat
    24001 ctgcacctgg atttccacct tcttttgcta attgctggtt tttctttgtc atgttttctt
    24061 ggattttagc catgacttct tcattttctt tgaccgcttg ttgctgataa tgagagatga
    24121 tctgctgatc cagataatta ttcagcgcgt cgctaacaaa tggataagct agtatcccta
    24181 ttcctgagag aagcaagaaa agcatcagca gatctagaag tttccttttc tttggtttca
    24241 tttgtgttct ccttgtgatg actgtttgat aataaaagaa aaaggagcat ttgcgtaatc
    24301 cgctgctcct ttttccaatt cttgcaaaaa gactaaatct gactgtgctt gcgtctagca
    24361 aagtacagtc cagcaatcag tagaaggact actcctgcac cgatatacac atagattccc
    24421 ttaccgcctg ttgaaggaag tgtacctttg tgtttatttg gtattttttc aggagaaatc
    24481 aactgtcctg ctgttacata agattgttca tcgatagtaa atgctacacg gtttgttaat
    24541 ggaacatatt tttctggcgc tttcgtttct tccagatagt acgtgccata ttttagacct
    24601 gtcacatcga ttaaaccgtt acttgtggtt gtaaaaaccg ttgctgattc tttcgccgat
    24661 acccagctga cggcttttgt ggatgggtcg atcgataaat atttcgctgt gtcactatct
    24721 tgatcacgaa cgacgaattc tgctccagca agtgtttggt ctgatgtaac gtcaccatct
    24781 actttaacaa atcgtttgcc cccagtaacg acatcgactg acggtggtgt ttgatcattt
    24841 gtatgcccgt tatcgacatt tgcttggtta gaaaatcctt tggttggatc tgctttttcg
    24901 ttcaaatgca tatagtaaac gaatttcaat gtaccgccag gagttaatga aggaatataa
    24961 ttcggatcaa ctgaaaccgt gaatccgtct gtttgctcag tgacagaata atttactggg
    25021 tcgatttctt tatttccatc atataaggca taagcatacg ttcctgaaga atcattatca
    25081 aatgttaaag cagcgtcatg agtatcgata agtttgaatg ttgtgtactt gttttgcgtt
    25141 ccttctttat cagcgatacc taatgggata ttgacagaaa tctcatattg aattttttca
    25201 ccgattgcga catcttttcc attcaattga ggtgttgttt tatcaacttt agacgtatca
    25261 tttttgatgg tcttttctac tggtgtttgg ctatttgcca ggatctcaaa aggcgtgatt
    25321 gtttgctttt caatcatttc cgcattatca ggagctttta cttcttctaa attatatttt
    25381 ccaacttcta aatgattaac gatcaactgg cctttttcaa tagatgcctc ggcaaagtca
    25441 ttgttgccga tgtcataaga atgaccagta atgaaatgtt tggctttggt ttgatcagtt
    25501 gtccaagtgt acaatccatc tgtgacactt tggatgtatt ttttgacgct tggtgttcct
    25561 tcttctttag aaataataaa ttctgctcca tttagtgctt cgttttcggc agtaccgatt
    25621 tttgtaactt tcaacgttcc atcattaccg actgtatttt tagggtagag atggatagta
    25681 tctagttctt ctgtcccgta tttataagag ccatctgctt gtttgatcat ctcatataca
    25741 gggaaagcta aaaccatgtt tgcggcagct gacactccgt cttttggttc ttctttgatc
    25801 gtgtagactg catctttccc attttgtttt ttaggtaaag ataaagtgac atttccatca
    25861 gcatctgtcg ttcctgaagc aactggtgta ccaggagtca aagactggac tgcttgtttt
    25921 gctgcatcca cggacgctcc tttatctcgt tgcgcataaa attcttgagt gacgttataa
    25981 actgaaaatg aaatatcggc taatccttgg tattgatcga attcgctcat ttctttcccg
    26041 ctgttttgga ttaaaggatc gggtaaatca gtcattttct ttttatgaag gatgacttga
    26101 gctgctgtct cctctgcaaa cacttttttt gcgccgaagc tgtttgtgag taatggtaaa
    26161 ataaggaaaa ggactcctaa cataacgttt atttttttat gatttttcat tttttcactc
    26221 ctgttctttt tttgataaac aaccagattg caaagcttgc gaataccata ccaacaacgc
    26281 ctaaaagtgt atttttcgtt tcgtttgttt ttggaagaaa tggacggcta ttttcttttt
    26341 tattgactac tttcaatata ctgtccgcct ctttatcctc agaaagaaaa gttgaaatgg
    26401 taaacggaat ttctgtttca cttaaaatat aaccagaagg tgcttcgatc tcttttaatc
    26461 gataacttcc tgttttcaac ccggaaattg aaaaagcgcc atttttatcg gaaatcaggt
    26521 gaagcgcatc tgattcgttc tttgtccact tgtatccgtt ggctgtttgc tggaggtaat
    26581 ttcctgcttt gtttttaata aggaatactg cgccagctaa tcgtttatct ggtttatttg
    26641 tctcgatttt gacaaattgc ttactccctg tatatacaga gactttcttt gtactgacac
    26701 caaaaccaaa atccagtgtt ccgttgttgt tgatttcctt gttcgcctgt gctgtactgc
    26761 ttagctgcat ctgataagaa atggtcagtt tttttccagc atatttttcc aacgaaggga
    26821 tcgaaaataa aacatcaaat ccgtgaggtt tcttgtgtat gacaaagcct tctgtcagct
    26881 tttctccatt cgatgaaatc gttaaactat taggtaaaaa cgttaatgct tcatccgcac
    26941 tatctgacaa ttcgaacttt tgatagtcaa ggatatttac aggaattgtc gtatgcaagg
    27001 aataagtgat tttttcacca atcgtaaaat catttctagg ctcttctaat accttctcaa
    27061 aaggtggatc agggtagtcg ttttcttcat ttttaggata caaatggata gttgaaagtt
    27121 tttgattgtt ttgtccatgt acaggaagaa caactaccat attctctgcc ttttctttga
    27181 cgacttctgg tactttggat tcaatgaaaa gatacgcttt atctcttttt tcttgatctt
    27241 tagcggccag agaaaaagaa gcgataccat cttcgttgtt aagagttgta gttgttgctt
    27301 ctgcggtaaa cataccggaa gacgcaccgt tttttgcgat ctctgcttgt gcttcttcta
    27361 ccgttttgcc cttttcccgt agatggtaaa aagaatctgt gacatcataa acttggaatg
    27421 tgacaccatt taatcctcga tacgtttgta ataaagcttt ttcttgtccg tcatttggat
    27481 gatttttcgg cagttgcccg tttgggaaaa gcaatttgtg taaagtaatc tgaaccattt
    27541 ctgtttctgt ttctgttgct acctgagtaa aagctggttt aaatagtaac aagaagagac
    27601 acatactaag ccaaccaagt tttttcatat ttagctgccc cctctttctt ttttcaaaaa
    27661 aagataccaa atagaaaacg cacaaccaat catccctact agatagattc ctaaacggcc
    27721 aattcctccc gtttcaggta atggtacttt tgcctgattc gtgatgtcta aagaaatctg
    27781 gttgtttttg gctcctggtg acagaacaga ttcatgatct tgtccatcca cttgaattga
    27841 cccatcttcg tgtataacta tagtaactgg ctcttttaga ccttggtatc cttctggtgt
    27901 aaaagtttcg gtcagcgtat aagttccagg ttttaaattt tcgaatagaa aggtatctgt
    27961 ttcttgtcca tcttttggcg attctaagtc catttctggt ccctgcagtc tgaattttgc
    28021 tccttttaat gtctgtccgt tgtcttcttt tttgtgtacc gttagatcaa acgcttttag
    28081 tcgattttga tgagtgagcg tccatacatt atttgtcatc tcggtatttt ttcccgtgta
    28141 aatgattttt ccatatttat cgatttggaa ttcataattt cccggcgaag tatcatatcc
    28201 ggcaggacca gctgtttcaa cgactcgata tttaccaggt gtgagtgaat caaaactggc
    28261 aagacctttt tcattcgttg tttggctgtc aattggctga taagtaccat ttgcttcttt
    28321 tttctgtagg gaaaatgctg ctcctttaag atttatctct ttgtccgtcc cttgcatcgc
    28381 atacttacgt actgctacag gaacttcaac aaaaggattt tcaatagtca actggatcgt
    28441 atcatcggaa gtagtgactg tttttccatc aatggttacc gtaccatcag aagcgatctt
    28501 gatttcccaa gtagtctttt tgcttaatcc atgcccttct ggagcttttg tttctgtcag
    28561 cgtataggtc atttcttttt gcaatttgac attttctgga agagaatagg ttccgtcgcc
    28621 atgatctgtt agtaaagtat caatagaatc tcctgttaat ttgaaaacag cgccaataag
    28681 atccttttca cctgtagagg aatttttcgt tattttcaag tttaacggtg tgaattgttt
    28741 agtatttttc catgtcattg catctatttg ttgagaatcg tatcctggta caggtaattc
    28801 tttgattgtt tggtaactga atgcttgacc ttgattattg tattgaggta gtgataatat
    28861 ctcttgataa ctttctccgc tatttgcaga taatttgtca atgtctgcac gttcccacgt
    28921 atttgttgta tctttagctg gtttaatgat tcgaatatat ccgtttttcc aagctgcagc
    28981 atttgtcgta tgttcccgtt gaatctcaaa agtaacttga tctggacgat cagctagatt
    29041 gttgtcaaat tcttcccata actttttgat gtgaagactg actccgggag ctttagcaga
    29101 tggtataccg aattcagcta attcattggt atcaatgttt ggctggaaag ttgtcctgcc
    29161 gttcatttga taccagaaat ttggatggaa gtcctcattt tctgtttgga ttctcacttg
    29221 gtaatggatt tggatttctt ggtcttttcc taaatagatc tgattgctct tgatggtatt
    29281 tccttctatg gaaatagatg gagtgaccgt tgtaggactt gtccccacac tcttgactga
    29341 aagtgtacca ggctgataaa tgaatggttc tgcgattgga tcatttattt gtccattgct
    29401 tacagttgct gagatctgta cagctttttt ggctaggtat tcagagatat ctgttgcatg
    29461 atcagctgat tcatagtaaa gatcgccttt ttcatctgat gaaaccattt gtcgcatacg
    29521 agactctact tctgcttttg agagaccagc tgccggatcg ctttgaagtt ggatgccaag
    29581 accatgtatt tcgattcctc gttctttgag tgccatcgct tctccgatgg ttgcgataaa
    29641 cgtactgtcg attcttctgg atagattgtt ttggtcaggt gcatcataga ttcttgagat
    29701 tagagaagta tttcccggcc gatcttgcgt attagaaaac tgagttccgt aataattgct
    29761 gcttgattgt gcgtgtaccc gctgtacttt ataggaaaat gttggtacac catccgtcag
    29821 caaaacgatc acttttttat gtccatttgg aacggatagc atgcttcctg catctcttag
    29881 tgctttttgt gtaaaagtac caccatttgt ccgtgaaggt gtaatggatt ttacttgatt
    29941 tttcactgaa tcaaatgaac ccatctgtac tgcaccgtta ctgtagctat atccttcgct
    30001 tgagtagccg acatatccca tattgatttt gtctgtgata ccgctatctg ctaaagtatc
    30061 gacaaaacga tcgacaccaa tctttacttc accgatccga ttattgtcgt tcatacttcc
    30121 tgaccagtct acgaccaata cgagatcaag aggcgtgata tctttttgta catttccacg
    30181 agcattcaaa taaacatcaa acaatccggg tgtacttgtt tcctttgcat actttcgtat
    30241 cgcatagtct gcttgattac cggttcctcc atattcgata tacgaatggg tccgatcatt
    30301 aggatctcca ttccaagaat ttataccatc ccattgattt gttccgtttt tattgccttg
    30361 atgatcaaga acatttgtat ttcctgtagg ttgccaactg gctttaggat aagtgccctg
    30421 atcatctgtt gtatattctg taggaatcgt ttgattagat ggcacactca cagctggcaa
    30481 ggataatgta ggcaatgtaa atgggctgac agaattttcg ttttcttttg atgattcttc
    30541 ttctatttcg tctggctttg gtactgacac atctgtatct ttcttatttt ctgtctcatc
    30601 aataatgtca tattctttat tatcgacgat cagcttgttt gcctggctgt ggtaaagtgc
    30661 tggatcgatt ggaaaactca gatcgatagt ttgtttagtg tttgatgaga tttccaacgt
    30721 aattttcccg ctatttacct gataagcata gccatctcct tcttttcttt ccacatcact
    30781 taagtaattt tcgactagac caatttcttt ttctatcttt tcagcactgg tgttattcgc
    30841 atttagcttg atgttcagtg ctttttttcc agactgatcc gaaatgactt cagctgaaat
    30901 tgtttctaaa actgttggat catctgtagt gtctgctatt gcagataatg gcacaaagtt
    30961 tgataaactc aatattatca gcatgaaaat aactttcagt ttcttccctg tggtcgtcat
    31021 ttttcccctc ctttttcttt ttttctgttt tgacgtgaat agtgaaaatt attaaaataa
    31081 acaggcgtta cataatttta tttattttaa tatgataatt attatacgaa gggcgctaaa
    31141 gggaatcaca ccaattttca taccaattgg tttcatttat gaagttgatt ccttcgatta
    31201 atgagcgttt cattgaaaga aaagggttga gggagaaaaa catatgatca ggttgttgga
    31261 aggaaactat agaaagatgg aaaaaatcat tcgaattctg tccatttctg acaagtggat
    31321 gactaaaaaa gaacttgcca aacgaattgg tagttcagaa tctacatttg taaggtatat
    31381 cgaagagatc aaaatccgct ggggaacgga tgtaacgatt caatcttcac aaaaattggg
    31441 gtatcgtctt gaacatttga atgtaccagc ttatttaaat gttctgatgg aaatgtcgca
    31501 aagttctaca aacagtcgtt tattaaatga gattattgtc aatccgggta aaacgattga
    31561 gtattattgt gaaaaaattt ccatcagccg gtcttctttt acacgtaagt tgaaacaatg
    31621 caatcaaatt ttagaaagct atttattaaa agtgatcgtt gaccaaggtt ttcagttaac
    31681 tagccttaaa agtgagctcc agttgcgtat ctttgttacc tttttctttt taagttacta
    31741 cggaagacgc acgttgccat ttccaatcga aaaaaagaag atcaaagaaa ttatggtcaa
    31801 gaataattgt cagatgaatt tgtttcagga agaaagccca tatgagcaat ctttctttat
    31861 tatgtatttt gttgtgcatc tcatgagaga atcccaagga actccatttg aatggaaagc
    31921 tggagactct tatgtacagt tgataaatat cgataaaaaa gacttcttaa tcttgaaaaa
    31981 tctcttagga aaattgaagt acagcagcta tataaaagta gtgaactatt ttgtcaatac
    32041 gattttttca gaattttcgg agaaagaaaa aaatgaaatc agtgagcaga tacgaaaaaa
    32101 tatgtcctac tgttcgtttt catccgctga ttgcaaagaa aaaaacgaag aatatgttgt
    32161 tcaattatta acgcaaattt attttttctc ccgtattttc ccatacgata cgactcattt
    32221 gacgatccgt gcaacttact ttgcacagca gatgcaacac cgccagatac gtctatatgc
    32281 tggatttcgt gcagaacttc aattttattc cactctactg aatcagaatc tgatttatta
    32341 tttatctagc tatatctttt ggatgcttat cggtaatcct gaaattcacc attatacttc
    32401 tgataaaaaa atactgatta tcagcgactt aagtcttcga catagtcaat atattgaaga
    32461 atatatcagt gatattcttg ctgtgcataa aattcactct gagacaactg ctattactga
    32521 agatcagctg tcgaaataca atcttctcga gtatgatctg atcgttacga atcaacccat
    32581 tttgaatgcc catgttcctc atatattgat tgatgatagt gtgtcattcg caaatgaaga
    32641 agaactcatc cgcttattcg agttatgaat aatcaaaaaa cagctatgta taaaccaccc
    32701 tgataaaaat ttcaggacaa atacacatag ctgtttcttt ttattgtatt ccttcagcaa
    32761 tccaacgaga tttatagcga aagtctttta acaactcttc ttttgtccaa ttatttgcaa
    32821 tgagatccgt caaagtgttc attacgaggg tcatggaaaa gtataggaaa ctaactgctt
    32881 cttccgacga ttgaatggag aaatgattga ttcttaataa ttccagccat ttttcgttca
    32941 ttggagaatc agccgatagt tctctacgtt tggttgtctg ctgatcattt cgctgggtta
    33001 acaagtataa cctttgccaa tagatacttt cgtgatctaa gtcgcagcac cactctaaat
    33061 atttttcgat ccccaaaaag aaatgttgtt tgttttcgct aatgaatttt aagatatctt
    33121 gatggactat gtgagaactg cttttgatcg tatacaaata agcatcttcc aaatcttgaa
    33181 aatatttata aaaagcaccg cgcgacatcc ccatcttgtc aacgatttct gagactttga
    33241 cttggctgat gtgctgtgag caaaatatct ccagtaatag gtcatcgatt ctctgtttct
    33301 ttgcgttcga taaattgaaa aaagtttctt ttggcatcca catctctcct ttatctctga
    33361 ttatacgtgg atagcccttc attgtcatga aggattcatt ccttcttttt ctcttcttct
    33421 gtcaagtacc catcttccat tcgataaacg cgatcactcc attgaatcat acggacatca
    33481 tgagtaacca taatagtcgc tttttgtttc tcatgagctt ctttaactag taatttgacg
    33541 acttcatatg catgatctgt atccaaacta gctgttggtt cgtctgctaa gatcaagctt
    33601 ggctcattat ataaagcgcg agcaattgcc actctctgtc tttctccacc agaaaggtct
    33661 ttaggataac tatttttaag tgactggata tctaaagatt gcagcaagga atcgattttt
    33721 gttgaatctt ctttttttga agtaacttgg tcaactagtt caaattgttc ctttaccgtt
    33781 aaaaaaggaa tcaaattaga gctttggaga ataaatccta tttctttaaa gcgtaaatct
    33841 gctcgttttt tttctggaag ctcagttagt gagtgaccgt tgatcactat ttctccggaa
    33901 gtcggtgttt gcaatcctcc tgaaatggta aggaaagtac ttttacctga accagaagga
    33961 ccaatgatgc tgacgaattc tccttccttt acataaaaat caattgcttt caaagctgag
    34021 acctttgtat gaccagaacc aaatgttttg gtaacttttt tcatcgttaa tatttttttc
    34081 atttttatta acctcctatt gctgtaattg ggtctacttt ggttatcgta cgtatcgaaa
    34141 acaatccgcc tagcattgcg acaatgatca aaatcccact atatagcaac cattgtgacc
    34201 aaaaaacagc aaaaggcatc gcgcttggta aaatcagact agtcaaataa gcaaacaaaa
    34261 gagcaagcag aacgcctacc accccaacaa taaaggactg tgctactaaa gatttagcga
    34321 tgaagaaatt tttgatccct tgtgctttca ttactccaaa aatagcagtc ttctgaagtg
    34381 tcatcacgta catgaaaata ccgaccacgg cagcaaccac taagaacaaa aagtatatca
    34441 tcgcatttaa ggtcatattt tgggcagaat agccaggaat actttcaatg aatgtcccaa
    34501 tagttaataa ctgtagaccc ttcgatgctt tttctttcga aattttagct gaatccttta
    34561 ttacgatccc attgatcggc tgttcttctt cagaagcaaa cggctgttgt ccatacttca
    34621 aagcagtcca cgtatctagg gacgcataga tgacgggaga aactgtgtaa taagtttcag
    34681 gaaagatacc gacaatcgtc aaaggatcgt cgtaactgcc aattttgatc gtatctccaa
    34741 ttttatagcc tgaatcagcc agattttgag agatgatgat ttcattcttt gttttaaatt
    34801 catgcccttt cgtgagtttt ggtaataaaa atgcttcttt cgttgtgccg aaaactgtaa
    34861 cattttcttt gttttttcct ttgattgctc cgctgtacaa tccgattggt gctttctctc
    34921 ctccttcaat gtatttcaga tctcctcttg ttaattgtga agctgcaaat acttgatttg
    34981 catcctccga caaaacaatc tcttgtgcgt cccaatcatc gattgctttt ttaaactctt
    35041 ctgaaagtcc attcgctagt cctgacaaca tgaatacgac atacgcaatc aagaacataa
    35101 taccgacgat caagccatat cgtaatttag aatagcgaat ttctttgata gctagaaaca
    35161 tttgtttttc ctccctatga tagattgata gtgacaaaat gtcacttgtt gaatagtgta
    35221 actctaaagt gacaatttgt cacttgtgtt gtttttttct tatcaataaa ttgtctaaga
    35281 catctttcga taaaacctta acatgagtca taaaaaattt atttaagcct tatttatgct
    35341 ttccatgata aactaaagtg accaaatcaa tgaggtggga aaatgattaa actattagta
    35401 gtagaagacg ataaagtatt atccaataat ataaaagaaa tactcgctgc aatcggcgag
    35461 ataacacaag tttatgatgg tagtgaagga ctgttcgagt tggaaagtgg agtttatgat
    35521 cttgcaatct tggatttgat gatgccggaa atgaatggat atgaagtgtt agcaaaaata
    35581 cggaaagaaa aaatacagac tccagtattg attttgactg caaaagatag tttggaagat
    35641 aagattgaag gatttgaacg aggtgcagat gattatctaa caaaaccttt ctatcgagaa
    35701 gaactgatca tgcgcgtgaa agcattattg aaacgctcgc taggtctctt tgatgaaaat
    35761 gttctgaagt ataaggatat cagttgtgat ttaaaaagca atgaagtctt gtataaagaa
    35821 caagttttgc ccatacaagg aaaagagttt gaattgctcg tttatttttt acaaaataag
    35881 ggaatcattt tgacaaaaga gcagatattt gaccggatct ggggatttga ttctgatacg
    35941 acgattacag ttattgaagt ttatatgagc aacttacgaa aacatctgcg aagtactgga
    36001 ttggataaag aaatcaaaac cttacgtaat gtcggttata tactgcaaag ggtgtgatgc
    36061 aatgaatgga gatattacac gaaagcaacg tagtcgcttt tttgcgacaa atgtagcagc
    36121 atttgctttt atcttttttc ttttgggatt cattacttta cagatattaa accaatctgc
    36181 atacagagaa acagatctgt cattagagaa aataaatttt gattctcgaa cagtacaatt
    36241 agagatacaa agatatcagc aaggcgatcc ctttttaaac ccgactcaaa cggaacaaaa
    36301 cagaacaaac atggaacagc cattaatgga accatcaaat gaccgtttta acactcaagt
    36361 gatcttgtgg tctaaaaatg gtgagatatt gaataaagca gctattggtg ggcgtttcaa
    36421 ccaaatcaaa gaattaaaaa tggatacgaa aaatctgaat accatccaaa atattgaatt
    36481 aaatgaagac agtggctcat ctgccctctc tttccgttcg attacgaaaa aagcggagac
    36541 gaatcaagat ggcgttgcct acatccaaat tttagaaaat acaaatcaaa tcaagaattc
    36601 attagcgact tttcgcacga ttatgatttt gtgtatgatt gttttttgga ttctctctat
    36661 tggtatcagt tattacttgt ctagtctaag catgcgccct attattcttt cttggagaag
    36721 acaaaaagaa tttgttgaga atgcttctca tgaattgcgc acaccgttaa cgattattca
    36781 aaatagtttg gaacatcttt ttaccaagcc taatcatact attattgaag aatccgaaag
    36841 catcgctcaa gcattgtcag agacgaggcg cctcactgga ttgacttcgg atttgctgac
    36901 aatcgctcgg aatgattctg atcagcagct gctttcaaag caaatgatca atacacaaga
    36961 atatatcgaa caacttgtta aaccatttca agagatggcc atcatagatg gaaaagagtt
    37021 tattctccat aacgatgcaa cattagaagt accaattgat gaaaaaaaaa tccatcaagt
    37081 tttggttatt ttgctggata atgcattaaa atatacgaat ccagaagata agatcatttt
    37141 ggattcatct gttaaccata aagaatggct gattcacgta aagaatactg gcccttctat
    37201 cagtgaagaa gatcaagcaa ggatttttga tcgattttat cgggaagatc aatctcgggc
    37261 gaaagagact ggcggatacg ggcttggctt ggcaattgca aaacaggtaa tcgaacagca
    37321 tcatggacag atttctgtat ccaattatct tccacaagga gtcgatttta aagtccaatt
    37381 gccgttgaag cctaaaaaat aatgcttatc cctaaaaaag ggctgtgaca aaaatcttgt
    37441 cacagccctt ttttgttgcg aataaacggt gttccagaag tagcttcttc ggaaataagc
    37501 cgaaatttct ttaagctgac cacttctgtc acaacctctt tagcttacgc ttacgatttt
    37561 ttattgcggt cttcaatgtg tacatcaccg cttgtgtcaa tatccaattc ttctcggcta
    37621 acatcttctg tcactttttc aacatcttca tgttcttctt tatgaatccc aacttcttca
    37681 cgaataactg tatgtttgct tacatctact tgctcttctt tgattgggat agtaatcgtg
    37741 tcatcttcca atttttcgtc tgttccctgt gaagattgat ctgttactgg tttgcgttcg
    37801 atgacgattt cttcacgttt gaccggtact tctaccgttt cagtatcgtt gaccacatgt
    37861 ttgtggatgt cgacttctcc agttgtcaca tcatgtgtat ttacatctaa tttttcttct
    37921 tttagttgaa ttgtatcatc atctagattt ggtttgcgat cgatgtcttc agtcttgctt
    37981 gtacccattg tatcagtctc gccaggtacg tccatcggat cagttcctgg tgtcatattc
    38041 aatggatccg ttgtattttg aaagcctgtc gtgccaccta cgtccattgg atctgttcca
    38101 gtgccagttt ctaatccagt ccccattgtg cttgctgggc taacagtatc ttgtgtatcc
    38161 attgtatctt ctgttgtttc tggctgacga tagcctttta ctgtgatgac aaattttcca
    38221 tcagaaatat ctcctctata ttgatataaa ggatcatttt ctggtgtagc cgtctcagaa
    38281 tcataggtat caaaagaaaa agtatctttt attttttccc ataaagaacg gtcttcttca
    38341 ccatccatcc gattttcatc tgtttcaaca tctataccag acaatccgtc aaatcctagt
    38401 ctagatgctg cttcatcagt agtataaata acgatatcat ctcgttcata cccttcgtta
    38461 cgcaaccgtt caattactga taatgcttct tccctcgtac tatagcttcc gatgaccgtt
    38521 aaatttttct cttccatgta aattcctcct agcaaataga tttatgattg gtatattgtt
    38581 acttataaat ttattctata ggagacgatt gttttctaca aatatttaga ttggaatttg
    38641 attaaaacaa tttaattgaa atttgaaaac atttatgtta atgaaagtca tattctaata
    38701 aaatgtcatt tgtttctata tgaattttta tgaaaaattt catataattt ccactcgttg
    38761 tgatggttta atttatccaa tccatcaata agcaaacaac ttatataaaa aaggattaat
    38821 tctcaatata attgctccta ccatagaata aatcttcatg taaagtaatg aagtaccaac
    38881 tatttctaat acttctttaa gaattatccc aacaatatcg ttagacaaac cattattgaa
    38941 taaatggaga gccaatcgat tctgaaaaaa tgttttctaa tttttcggaa ataaaagaaa
    39001 gagcctcctg ccattagaac gaaccaaatc catttcattg ctcatgaagt aaccacatag
    39061 ctaaaaagag gataaatcaa ttaaaagatc aaaagaatga ctgcactaat aaaataacta
    39121 aaaatgtatc ttcttacttc atatctttta gctagtactt agttaaaaat ttgcttccta
    39181 agatcaagac aaactgtcta tcttcatttg atgaaatgcg gtaaaatata gaaaatgaac
    39241 actattggag ggaatttgta atggacagca aaactagaaa acaactgcaa gaaaagttaa
    39301 agcaacgtac acacttgctc ccttccgtct ttcaacagtt tttagaagaa aatcgttctt
    39361 ccctttcatt aggcagtcgc tatgaatacg caaaagattt tcatctgttt actgattatt
    39421 tgagaaaagt attttctgaa gaagtggatg atcagctgat tatccattta gaaaaacaag
    39481 attatcagaa tttcttagct aaaatctatc gacatactcg ttcgttccaa actacagcga
    39541 accaagaaac ggagcaattg tttgaaaata gctattacgg gatttcaagg aagcaatatt
    39601 cattaaatca ttttttacga tttttgtatc aaaaaggact gattgaagaa gagatttctc
    39661 ttttgggtac aagtttaccg gaaacaacca aagcagctcc gcgatatatc gatgcagaac
    39721 ttttcaaaga ttttgagcat ttgtttatcc cgattgaagg ctttcaaaca agcagagaag
    39781 ctagctttga agaaaagaat agaccgagga accttgcaat tactgccatt cttctgtatt
    39841 gcggattaaa gatccatgaa gtagtagaac ttaaacgtaa agatgtatct ttgaccaagc
    39901 agattcttaa tctgaataga aaagaaaaca gactgaataa agtaccaata ccaaaagaag
    39961 cgatgccatt tctggagaac tattttaagt taacagcaca aaatacggac gaaaattctt
    40021 ttgtttttcg ttcctcacat gatcagcaaa tttctcctcg gactattcgc caaattttga
    40081 gcaaaattac agtatataaa aacgtatcgc cagaactatg cagaaaaaca tatcgttatt
    40141 atgcagaact atatttagat gatgctgacg cggtaaatta tttatgtggt aatcgatacc
    40201 tctcatccca ttcttttcaa gatatctggg aaaagatggt tgatttttct tatcacgaat
    40261 tagcggcatt cgttcgggtt tcttccatat aaaacaaatg tatgatcttt ttttcacaat
    40321 aaactatgtt attatataaa ggattatgtt acgattatct tgaatttgta tattacttaa
    40381 caaaagataa tggagtgtga atgatggaga ccataacgta ttcccctttg aacttattta
    40441 cgaattttca agaagctgca gctaactttt ccgagacccc gattatcttt gatgagccac
    40501 tttcagcttt cccagaatta ggacttgaaa caacttacaa aaaatgctgc gaaagtgtgt
    40561 tgaaacgggc ttatcaattg gctcatcttg gagtgaaatc cggagacaaa gttattattt
    40621 ataagagttc cgcttttgac acataccttc tagctgtttc tgtgtcttac cttggtgcag
    40681 tgcctgtcat gacttcttat catctaccaa caacaacaat ggaagtattt atcgatcgtt
    40741 tagaagaccc tttcatttta tttgatgatg agacaaaaaa gcgagtgaga gaaatttcaa
    40801 atggaacaaa atcaaaacag attcctattc tttatttaat ggaacaacct gctttgtctg
    40861 tttctcaatc atttttagac aaaaatgaaa tttcttatat gacccatact tctggtacaa
    40921 ctggtatccc taaacttatt tgtcattctg ctcattctat gggatggcgg acgaaatggc
    40981 aaaaaacagt tttcacaaaa atttcggaaa agaaattaat cgcttttcac atctctccag
    41041 tgcattccag attcaatatt ggtatctctt ctttgatgtc catgggattt cctatgatgc
    41101 ctttagcaaa cgcacaaagt tcaaaagttg ttcatatgtt ggaagctcac cggccaattg
    41161 cactagagac ccatccgaat aactttgttc agtggcgttt tactgctaag gagcatccag
    41221 aagctttttc gggtatccga tactatcact ccacttttga tgctataaac aaccagacaa
    41281 tgaagacatt tttgcgaacg tcgatggcac aggatgcggt atttcttcaa gtatatggcc
    41341 aaagtgaatg cggaccaatg attcttaagg ctcatacact agagagtttg aaaacaagtg
    41401 atgcaagaga tatgggcgtt ggactggaag acatgacaag cgctcggatt acagattcag
    41461 ttgggaatgt cctacctgaa aacacagatg gtcatataca attactgtct aaaggacggg
    41521 ctttgactta ttacaaggaa gatgcacggt tccaggaaaa tgtttatggc gattggtggg
    41581 atagcggaga ttacggtttt atggataagc atggccacct atttttgaaa gatcggcaag
    41641 tggatttgat cgaaacgatc aatagtacat tagctattga agacttttta ttagattcgt
    41701 tagatttttt ggcagaagta gtgattgtaa gggataaaaa tcattctcca caaccgatta
    41761 ttgctttagc tcctgataaa gaaatggatt ggaatcgatg gtgggagcaa gtccatgaac
    41821 ttcctcgctt gaacgtccct atcatccgtg attttgatgc aatcccgcga acagctacaa
    41881 tgaaagtgca acggcttcaa atcgagaaag aattaaaatc acaaaagtcc taactattta
    41941 aggaaagaaa gatccatttt ggtctttctt ttctttttgt ttttttaaat tggatgagat
    42001 aaaaatatgc caattggctg tttttctttt ttgaaaattt cgttatactc ttattcatgg
    42061 aggaaaatgt tatgaaaaga atcttgacaa ttggcggttc tgatccattt gctggtggag
    42121 gaatccaaac agatttaaaa acttttgaga attttggtct attcggacta agcgccttga
    42181 cttctatagg ggcactagat attaatgagc gatttatgct ggaatctata tctttggaat
    42241 tgcttaaacg acagttagtt tcgattgata aaatgacttc attagatggt atcaaaattg
    42301 ggttattgaa ttcaagtgaa gcaatcatgg ttgttcgaga cttcataaaa acaaaacaaa
    42361 atatcccaat cattttagac cctgttttgg cattcaaaga aactgaagct gtgatcaatc
    42421 aattgtatat ttcgcagctc atcgaagaac tttttccact tgttacgatg attacgccta
    42481 atttggcaga agctgcttta ctttctggtc aagctcttcc ctcttcgtta acagaaatga
    42541 tccatctcgc taaagaactc actgcttcag gaacaaaaaa cattgtgatc aaaggcggca
    42601 caaggatggc tggagatgaa gctgtggatc ttctctattc aattgatcat tatgaggtat
    42661 tttctcataa aaaaatcgac actacaacag taaacggggc cggttgtgcc ttttcttctg
    42721 caataacagc gaatcttgtg ttaggggcag attttaccaa agcagtagca gaaagtaaaa
    42781 aatttgttgc ccattgtatt tctaatggcg tgctgatgaa cgatcatacc ggaagtgtct
    42841 ggtctggcgg tattcaaaaa ggaggaatga gaaatgaaaa ctaagcaatt aacgatctat
    42901 gctatgctga ttgcgttgac agttgccctt tcattaacaa ttttgatccc tgtaccggct
    42961 acgaatggat ttgtgacatt atgcgaggcc gggatctata ccgctgcttt tctttatggt
    43021 ccaacgggtg gattagttgt tggtgcttcc agtggtctat tgatcgattt gatttcagga
    43081 tatccgcagt gggcagtttt ttcattagtg atccacgggt tacaaggata tgttgctggt
    43141 tctttcagca aatcttctac tcgtatgtgg ggattagggc ttttattagg aagcttagtt
    43201 atgatcatcg gctatggtat cgccggttgg attttatatg attggccggc tggagtggct
    43261 tctataccag gaaacatctt ccaaaatctg attggaatgg gacttgcctt tcctataact
    43321 gcaagtcttc aacgtattca agcacggaag caatattaat tgaaaaagaa ggttttagaa
    43381 aatgaagttg tcagaaaaag aattaaaaga ccaattgact gaaatagtta atgacatatt
    43441 ggctgaggca cacttgaaaa aaggtgatct ttttgtttta ggatgcagca caagcgaagt
    43501 cgttggagga catattggaa aaaattccag tgcggaagtt ggtcaatgga tcatccgtac
    43561 gttaaaagag ttgttggatc ctaaagaaat tgcgctagct gtccaaggtt gtgaacattt
    43621 gaaccgagca ttagtagtag aacgaacagt ggcagaagca aaaaatttcg aaattgtctc
    43681 tgttgttcct gcactacatg ctggaggtgc ttgttctgtt gctgcttttg accagttcaa
    43741 tgatcctgtc gaagtggaac acgtagttgg acaagccggt atcgatatcg gtgacacatc
    43801 aatcggtatg catatcaagc atgtccaagt acctgtcaga cctcgtttga aaacccttgg
    43861 acaagctcac gtgacagccc ttcgctctcg tcctaaatat atcggtggtc ctagagcgaa
    43921 ttattgaaat gagtaattga cagagcaatc tgaaaaactt cctcgcaaag tccaactaat
    43981 gaactttaac gaggaagttt tacttgtttg ttttatataa ccgagcttca taaggtgcta
    44041 gtatgccttt agcttcaaat tctccgctag aggaatttga caagagtaat gtccaggact
    44101 gatctgcttc ttttggaagt tcatagtcag caagtgattc tgttaagttt accataataa
    44161 gatattgttc atcttctaaa tatctttcat agacataaag ttgtggatgt tcatgaagat
    44221 attctttata agaaccgtat atcagacctt tattggtttg tctaatgcgg atcatgttct
    44281 tatagaattg caggaccgac tctggttcat attcttgttg ttctacattg atcgttttgg
    44341 tatttggatt agtgatcagc caaggttcat gtgtagaaaa accagcatat tgttccgaag
    44401 tccattgcat gggtgtcctg ctgttgtctc tcgttgtttc acgaatgata tccaatgctt
    44461 cttctcttgt cttaccttcc gcaagcagcc ttttataaag tcttttcgaa tctactgcat
    44521 caacttgatc gatcgattca aagggcatat tcgtcattcc gatttcctgt ccttgataaa
    44581 taaatggcgt accttgcaaa aagaaataca tcatagcgat tgcttttgca gacatagccc
    44641 aaaaatcggg acgagtatct ccaaacactg aaacggcacg aggaacatca tggttttcca
    44701 tatataaagc gttccatccg ataccatcca atgaagtttg ccaagcgctt aatgcatgtt
    44761 tcaatttaag gacatctaat tgcccttctt tttcttgctg ccagatatgg atatgatcaa
    44821 attcgaatat catatcgaaa taaccgtctt tccctaccca ttgtggtcct tcttctgcag
    44881 tgacaccact tgcttctccg acagtcatga tatcgtactc ttcaaaaaca tgtttcaaat
    44941 cggtcaaatg ttcttcgatt cctgagacat tttgaaaggg tgagaaaggg ttctctgtgg
    45001 cttttactga atattcatct ttttttacat gagaaatcgc atctaaacga aatccatcta
    45061 tccctttatc caaccaccat cggatcatat tgaatagttc ttctttcaat ttctcgcttt
    45121 cccaatttaa gtctggttgt tcttttgcaa aaacatgcag ataatactgt ccagtttcat
    45181 gagaatactc ccaagcagaa ccgccgaaaa tcgatgtcca attattcggt tctttgccat
    45241 ctaccccgtc gacccaaatg taataatcgc gatacgggct ttctttagat tttttcgatt
    45301 cttcaaacca ttgatgctga tccgaactat ggttgatgac aagatccata atcactttga
    45361 tccctaattc atgtgcttct tttagcaata aatcaaaatc ttccatcgtt ccgaatctct
    45421 cttctatatc ttgatagtca cttacgtcat aaccattatc gacaaatgga gattgataga
    45481 tcggattgat ccagataaag ccgataccaa gatctttcaa ataattaagt ttttcacgga
    45541 tcccattgat gtctccttga ccatcgccat tgctatcttt gaaactagca ggatagattt
    45601 gatagcctac tgcttctttc caccattttt gttcttgtat catatcgttt cactccttta
    45661 acggtccctc ttgattataa ccgattctat tttag
//
LOCUS       DARBET010000015        45528 bp    DNA     linear   BCT 07-NOV-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4027
            SAMEA114569841-rid20112183.denovo.015, whole genome shotgun
            sequence.
ACCESSION   DARBET010000015 DARBET010000000
VERSION     DARBET010000015.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA114569841
            Sequence Read Archive: ERR12203666
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 45528)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 45528)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001972618.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001972618.1
            Genome Coverage       :: 132x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/07/2023 13:18:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,041
            CDSs (total)                      :: 2,975
            Genes (coding)                    :: 2,908
            CDSs (with protein)               :: 2,908
            Genes (RNA)                       :: 66
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 67
            CDSs (without protein)            :: 67
            Pseudo Genes (ambiguous residues) :: 0 of 67
            Pseudo Genes (frameshifted)       :: 26 of 67
            Pseudo Genes (incomplete)         :: 28 of 67
            Pseudo Genes (internal stop)      :: 20 of 67
            Pseudo Genes (multiple problems)  :: 6 of 67
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..45528
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA114569841-rid20112183.denovo.015"
                     /isolate="VRE4027"
                     /db_xref="taxon:1352"
                     /country="Denmark"
     gene            complement(26..1522)
                     /gene="lysS"
                     /locus_tag="SCJ86_000929"
     CDS             complement(26..1522)
                     /gene="lysS"
                     /locus_tag="SCJ86_000929"
                     /EC_number="6.1.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysine--tRNA ligase"
                     /protein_id="HEG1168510.1"
                     /translation="MTQEQQHQVEDLNDQMLVRRQKMEQLREDGIDPFGKRFERTHNS
                     EELHELFDPRTKEELAEMGLTASVAGRMMTRRGKGKAGFAHLQDREGQIQIYVRKDQV
                     GDEAYEVFKHADLGDFFGVTGQIMKTDTGEVSIKASEITILSKALRPLPDKYHGLTNI
                     EQRYRQRYLDLISNRESFDRFMKRSQIISEIRRYLDGNGYIEVETPVLHNEAGGAAAR
                     PFITHHNALDIDLYLRIALELHLKRLIVGGMEKVYEIGRVFRNEGIDTTHNPEFTMLE
                     AYTAYTDFNDVMNLTEGIIRNAAEKVLGTAKITYDGQAVDLESDFKRIHMVDAIKEQT
                     GVDFWQEMTLEEALALAEKHNVEVTEAMGVGHVINEFFETFVEETLTQPTFVYGHPVE
                     VSPLAKKNPEDPRFTDRFELFIVGREFANAFTELNDPIDQRERFEAQEKERELGNDEA
                     HGVDEDFLEALEYGMPPTGGLGIGIDRLVMLLTDAQSIRDVLLFPTMR"
     gene            complement(1633..2637)
                     /gene="dusB"
                     /locus_tag="SCJ86_000930"
     CDS             complement(1633..2637)
                     /gene="dusB"
                     /locus_tag="SCJ86_000930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307694.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA dihydrouridine synthase DusB"
                     /protein_id="HEG1168511.1"
                     /translation="MEHTWKIGNVEIPNRVVVAPMAGITNSAFRVTVKEFGAGLVVCE
                     MISDQGIHFRNKKTLEMLHIEETEHPLSLQIFGGNKDSLVEAAQFVEENTAADIIDIN
                     MGCPVNKVIKAEAGAKWLLDPEKVYEMVQAVSSAVNIPVTIKMRIGWDEEHVFAVENA
                     LAAQSAGASAIAMHGRTRVQMYEGKADWEVLKEVKKHLTIPFMGNGDVKTPEDAKWML
                     DYVGADGVMIGRAALGNPWMIHRTQHYLETGELIPEPTPAEKIATAKLHLQRLADLKG
                     EKVACREFRTHAAYYLKGIPRAAKVKVAINQTEKQQEINDLLDQFVEKAEKVLLKQTL
                     "
     gene            complement(2638..3525)
                     /gene="hslO"
                     /locus_tag="SCJ86_000931"
     CDS             complement(2638..3525)
                     /gene="hslO"
                     /locus_tag="SCJ86_000931"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335306.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Hsp33 family molecular chaperone HslO"
                     /protein_id="HEG1168512.1"
                     /translation="MKDYLVKALAHDGFVRAYAVQATNTVAEAQRRHDTWHTSSAALG
                     RTLVGSLLLGATLKGEDKLTVRIQGDGPATGIVVDANGKGDVKGYIKNPHLSLPLNSE
                     GKIDVRGAVGTQGMFTVTKDLGLKEPFSGQTPIVSGEIAEDFTYYLAISEQIPSAVGL
                     SVLVNTDDSIKTAGGFMIQIMPGADEETISKIEEQLKTTPRISTLLDEGQTPEEILKG
                     LLGTEELNILEKMPVQFKCDCSKEKFAEAIIALGAEEIQAMIDEDHGAEAVCSFCNNK
                     YKYSEEELDGLKKEALGGI"
     gene            complement(3742..5853)
                     /gene="ftsH"
                     /locus_tag="SCJ86_000932"
     CDS             complement(3742..5853)
                     /gene="ftsH"
                     /locus_tag="SCJ86_000932"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008265922.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent zinc metalloprotease FtsH"
                     /protein_id="HEG1168513.1"
                     /translation="MNKKNNGMMKNALYYVLVILAMVMVVYFIFGNNGQRSSDIEYSK
                     FTEQLQAGKIKEFEVQPANGVYKITGEYKEQQTVANSGGLAILGSTDTTTTHFTTIIL
                     PNDTTLSQVTELAKENKVATTVKEESSSGIWVSLLLSFLPIVIIIFFFYMMMGQQGGG
                     GGGGGRVMNFGKSKAKEADKKANRVRFSDVAGAEEEKQELVEVVEFLKDPRRFVELGA
                     RIPAGVLLEGPPGTGKTLLAKAVAGEAGVPFYSISGSDFVEMFVGVGASRVRDLFETA
                     KKNAPAIIFIDEIDAVGRQRGAGMGGGHDEREQTLNQLLVEMDGFDGNEGVIVIAATN
                     RSDVLDPALLRPGRFDRQILVGRPDVKGREAILRVHARNKPMADDVDLKVVAQQTPGF
                     AGADLENVLNEAALVAARRNKKKIDASDIDEAEDRVIAGPAKKDRVISKKEREMVAYH
                     EAGHTIVGLVLSRARVVHKVTIIPRGRAGGYMIALPKEDQFLMTKEDMFEQIVGLLGG
                     RTAEEIIFNVQSTGASNDFEQATALARSMVTEYGMSDRLGPVQYEGNHQVFVGRDYGQ
                     TKAYSEQVAFEIDQEVRKILMEAHDKAREIIEAHRAQHKLIAEKLLEFETLDAKAIKS
                     LFEHGVMPEGTESNDYPSEKEAKTFEEAKRALEEKDAEKQAEEKRDFEEAKHELNEEN
                     KAEHDSDSQQSEDNHSDDQYK"
     gene            complement(5943..6488)
                     /gene="hpt"
                     /locus_tag="SCJ86_000933"
     CDS             complement(5943..6488)
                     /gene="hpt"
                     /locus_tag="SCJ86_000933"
                     /EC_number="2.4.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypoxanthine phosphoribosyltransferase"
                     /protein_id="HEG1168514.1"
                     /translation="MLEKDIERILISQEEIQVRCKELGKELTEIYQNTNPLVVGVLKG
                     AVPFMADIVRSIDTYLELDFMDVSSYGNATVSSGEVKIVKDLDTNVEGRDLLIVEDII
                     DSGRTLAYLVDLFKYRKAKSVKIVTLLDKPEGRVVNIEADYVGFNVPNEFVVGYGLDY
                     AEAYRNLPYIGVLKPSVYQSN"
     gene            complement(6488..7933)
                     /gene="tilS"
                     /locus_tag="SCJ86_000934"
     CDS             complement(6488..7933)
                     /gene="tilS"
                     /locus_tag="SCJ86_000934"
                     /EC_number="6.3.4.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287414.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA lysidine(34) synthetase TilS"
                     /protein_id="HEG1168515.1"
                     /translation="MNVEKGGKKAPLLQQSFIRHGNEQGFWQQGSRILAAVSGGVDSM
                     VLLHLLLQVQRQIGFSLGIVHINHQLREESAEEAAYLRSYCEEKNLPLYVSIWEDPAK
                     SAIETEARNFRYQEFTRVMEEGQYDTLMTAHHGDDQIETVLMKIVRGGQLNTFSGIKE
                     VQPFATGRLIRPLLSFSKEDLYTYAAESQLVYFEDQTNQLLDIQRNRLRHLVVPQLKQ
                     ENTQALRHFQQFSQQIQWADQVIQKYMGQLIEKEVEQWEEQFRFSAEMIEEMTDAERY
                     YFFYSFFDKAFSKTGIILKEQQMESILDQWQQKKSQWQLSIGNNWILKREYHFVVLSK
                     KEKQATSQTEDQRLLLIPEQGIYLNETEWIGLVKPENAGDIKATDSWSLFSHDIWIPA
                     DVQLYVEKRKAGDRIKLTENLTKKVSRYFIDQKVPNSQRQHAWIITDAKRNIYSLIPF
                     AFSYLSIREETDKIHYILLYKYQKEAIGRRT"
     gene            complement(8053..8523)
                     /locus_tag="SCJ86_000935"
     CDS             complement(8053..8523)
                     /locus_tag="SCJ86_000935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287412.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-binding protein S1"
                     /protein_id="HEG1168516.1"
                     /translation="MSIEVGAKLQGKVSGITNFGAFIDLGAGKTGLVHISEVSNGYVK
                     DIHDVLSVGDEVTVKVISVNDDGKIGLSIRKAQEQPAESGEESRPKKYQQKDPRDNRN
                     RPAQKKSFSRSQPSAQKEDFDSLMSSFLKDSDDRLSSLKRNTEGKRGGRGGRRS"
     gene            complement(8569..8994)
                     /locus_tag="SCJ86_000936"
     CDS             complement(8569..8994)
                     /locus_tag="SCJ86_000936"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287409.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="septum formation initiator family protein"
                     /protein_id="HEG1168517.1"
                     /translation="MTKKSNKIAALDTEYAKKKYAEFQQQQRQLIFRRRRLAAIFVGA
                     LVIFAIIGVQIFHDMQRMHQLNELRVEANTEMKEVNADVDQLHQDVSLLKDDEYVAKL
                     ARSKFFYSKDGEKVYPILESNTDSSTNETDQSTSSTTAK"
     gene            complement(9061..9330)
                     /locus_tag="SCJ86_000937"
     CDS             complement(9061..9330)
                     /locus_tag="SCJ86_000937"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287407.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-binding S4 domain-containing protein"
                     /protein_id="HEG1168518.1"
                     /translation="MRLDKFLKISRIIKRRSVAKEVADKGRIQINGKLAKSSSDVKVG
                     DILKVQFGNKTLEVKITELHESTKKEDAQKMYEIISENRTETLEK"
     gene            complement(9341..10936)
                     /locus_tag="SCJ86_000938"
     CDS             complement(9341..10936)
                     /locus_tag="SCJ86_000938"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide biosynthesis protein"
                     /protein_id="HEG1168519.1"
                     /translation="MDHKEMRRVMQGAFVLTIASFIAKVLSALYRIPLQNLVGDEGFY
                     VYQQVYPIYGIAMTLALSGLPQFISKYVAERKNPFERKQALQNLYPLVFWTGVFLWLF
                     VFAFSSWIAWMMGDIQLKPLIQVVSFTFLLMPGLSFYRGNFQGRFLMEPTAVSQVVEQ
                     FVRVFVIVCSAFAFRKLGWTVYQTGTAAMSGAVFGGLCAYGILHYYEQKIHGGALSFR
                     RFPLFKQPSKPLIRRFLVEGGLVSIYSGLLIFFQLIDSFFVKNALEVYGLSEYGAKIA
                     KGVYDRGQPLVQLGLVIATALSATFLPALTRHLTNRIYRQFLQTAKIYLRLTTALALA
                     ASLGLALLLPYINYALFKDYAGNAALVLFVFSIAFTAVIQAYQSIAQSKNSFRPSLKG
                     AGWGLLVKGLTTSFLTGLLGTAGASLSTLLGLGVTLWYFVRIETKELNAFWKERHFGR
                     KLMFSLLCMTVVLLLYYGVLNIVFGPVTHRSTAFFVSVIGVGIGASVFIYAVIRLRLF
                     TVREWLMLPFGKKILRLNKKIKK"
     gene            complement(10951..14472)
                     /gene="mfd"
                     /locus_tag="SCJ86_000939"
     CDS             complement(10951..14472)
                     /gene="mfd"
                     /locus_tag="SCJ86_000939"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription-repair coupling factor"
                     /protein_id="HEG1168520.1"
                     /translation="MDIIQLVGKDPQVKEWVSNLEEKMPRQLITGLAGSAKTLLFARA
                     FKKTNKNMIILMPNLYYANQLAEDLQHVIPQDQIHLFPVDEVLSAEMAFSSPEARGER
                     VATLNALQEKKAGIYLLPVASLHKRLPNKKTWLQAQLNWHIGDEIDVENLPRQLTLMG
                     YVREEMVAKPGEFSIRGSIIDIYPLTTTYPVRVELFDVEIDSMRYFEAETQRSIEKTE
                     EIWVSPTSEQVYGPEDLEHGIEQMQKMLEKRLAATTEEADREFLQDYFGQLTSSWQAG
                     VPTEEAKFYSDLLYQESVTILDYFPSNSLLVVDDYQRIMETNREIEREAAEWQTQKIS
                     ELRVFSEQTFSADIQKIVQKEKFTTTFFSLFQKGMGNLRFQAIHNFQYRTMQQFFGQM
                     PLLKTEMDRWRKQKQTVVIFIPTKDRIRKAEQMLRDEDILVVETEKNALIRGQVQLVE
                     GALQAGFELPQEKIVAITEKEIFQKTTKKQTRRQTVTNAERLKSYNELKAGDYVVHAN
                     HGIGKYIGMETLEVDGVHQDYMTILYQNDDKLFIPVTQLNLIQKYVASEAKAPRINKL
                     GGSEWTKTKRKVSSKIEDIADDLIKLYAARESEKGYAFGPDDAYQKEFENAFPYSETD
                     DQLRSAAEIKRDMEKEKPMDRLLVGDVGYGKTEVALRAAFKAIKESKQVAFLVPTTIL
                     AQQHYETMLERFEGFPVNIGLLSRFRTRKQQKETIEQLRTGQVDIVVGTHRILSKDIE
                     FSDLGLLIIDEEQRFGVKHKERLKQLRAQVDVLTLTATPIPRTLHMSMLGVRDLSVIE
                     TPPANRYPIQTYVMEKNPGAIREAIHREMGRGGQVFYLYNRVETIEQKVEEIQELVPE
                     ARIGYAHGQMTEAQLENTLFEFIEGQYDVLVTTTIIETGVDIPNANTLFVENADYMGL
                     STLYQLRGRVGRSSRVAYAYFMYEQQKILNEVSEKRLQAIKDFTELGSGFKIAMRDLS
                     IRGAGNLLGAQQHGFIDAVGFDMYSQMLSEAVSRKQGKNSQVEKTTVEIDLGVDAYLP
                     ETYVADQRQKIEIYKRIRELDSQEMLDELEDDLLDRFGEYPEEVAHLLTIGQIKMDGD
                     RALLETIRKQEAHIVFTLSKVGTKRYSVEQLFEALTATKLKASLGVEKEQMVIRLTIP
                     NQMEEAVWLQEIQKFVKALREQKYLSA"
     gene            complement(14489..15049)
                     /locus_tag="SCJ86_000940"
     CDS             complement(14489..15049)
                     /locus_tag="SCJ86_000940"
                     /EC_number="3.1.1.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348722.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminoacyl-tRNA hydrolase"
                     /protein_id="HEG1168521.1"
                     /translation="MKMIVGLGNPGTKYQYTKHNIGFMVVDKIAREHQATFKKNPFEA
                     EVAEFFHNGEKILLVKPQTFMNESGRAVGPLMTYFGIYPEELVVIYDDLDLAVGKIRL
                     RQKGSAGGHNGIKSIISHLNTNVFDRIKVGIGRPEGKKTVVQHVLSPFSKENQPLIEE
                     SMCQSVKAVEYLIEGHSFVDAMNRFN"
     gene            15402..16376
                     /locus_tag="SCJ86_000941"
     CDS             15402..16376
                     /locus_tag="SCJ86_000941"
                     /EC_number="1.1.1.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875171.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-lactate dehydrogenase"
                     /protein_id="HEG1168522.1"
                     /translation="MTAAAEKKDHQKVILVGDGAVGSSYAFALVTQNIAQEVGIVDIN
                     TAKTEGDAIDLSHALAFTSPKKIYSATYADAHDADLVVITAGAPQKPGETRLDLVNKN
                     LKINRDVVTQIVESGFNGIFLVAANPVDILTYSTWKFSGFPKERVIGSGTSLDSARFR
                     QALAELVDVDARNVHAYILGEHGDSEFPVWSHANVAGLQIYEWVKNNPEIDEEAMVNL
                     FFGVRDAAYTIIEKKGATFYGIAVALARITRAILDDENAVLPLSVFMNGEYGLNDIYI
                     GAPAVINRQGIQKVIEIPLSDSEQDRMAASAKQLKEILDEAFAKLDAE"
     gene            16546..17946
                     /locus_tag="SCJ86_000942"
     CDS             16546..17946
                     /locus_tag="SCJ86_000942"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L,D-transpeptidase/peptidoglycan binding
                     protein"
                     /protein_id="HEG1168523.1"
                     /translation="MTRSSHRKKSKHVALLSVLGVLLVLISFYTYRSTYYTKHFLPNT
                     EINGINVSNLTVEKANEKLKEAYSDKKLAIKEDGKVWQEVPKSELGYKDDFTSELSPI
                     LNEQNGWTWGMTYVSAAEKQEIDPVSQNHQKLDTTVQTLTTKLTDLNKDRTPTTDATI
                     EKAGDSFVIKPEVNGNTIDVDAAVKQLKSAVNSGKDTIELTEFKEKPKVTSEDSSLKE
                     QLASMNAIANVKATYSINGETFQIPSSDIMSWLTYNDGKVDLDTEQVRQYVTDLGTKY
                     NTSTNDTKFKSTKRGEVTVPVGTYSWTIQTDSETEALKKAILAGQDFTRSPIVQGGTT
                     ADHPLIEDTYIEVDLENQHMWYYKDGKVALETDIVSGKPTTPTPAGVFYVWNKEEDAT
                     LKGTNDDGTPYESPVNYWMPIDWTGVGIHDSDWQPEYGGDLWKTRGSHGCINTPPSVM
                     KELFGMVEKGTPVLVF"
     gene            complement(18024..18746)
                     /locus_tag="SCJ86_000943"
     CDS             complement(18024..18746)
                     /locus_tag="SCJ86_000943"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346894.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YebC/PmpR family DNA-binding transcriptional
                     regulator"
                     /protein_id="HEG1168524.1"
                     /translation="MSGHSKWHNIQGRKNAQDAKRGKVFQKLSREIYMAAKAGGPDPS
                     MNPALRLVVDKAKAANMPNDNVERAIKKATSAVDETNYDEITYEGYGPGGVGILVHAL
                     TDNRNRTATNVRVAFTRNGGSLGETGSVSYLFDRKGYIAIERDGLTVDEETMFEDVLE
                     AGAEDLQTSPEVFEIYTAPEDFAAVRDELEKEFTLAQAELTMIPQTTIDLNEEQKEQL
                     ERLVDKLEDDDDVSEVFTAAEM"
     gene            complement(18825..19331)
                     /locus_tag="SCJ86_000944"
     CDS             complement(18825..19331)
                     /locus_tag="SCJ86_000944"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331288.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VanZ family protein"
                     /protein_id="HEG1168525.1"
                     /translation="MNSSKKQMKNGNIYLVIALIVVIILFYSSSQTYGQQSQVSRLES
                     WLPNQPFKDTLAGIRFNYGGSMVSINHLGYAKFLEFFLRKGAHFGTYFILGGSLFLGV
                     FPKLKIWWLTAILAWLSATGYAGLDEFHQMMTGGRTPMFQDVMLDSIGALTAVILCVL
                     FAAFKKRL"
     gene            19454..21253
                     /locus_tag="SCJ86_000945"
     CDS             19454..21253
                     /locus_tag="SCJ86_000945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629808.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M3 family oligoendopeptidase"
                     /protein_id="HEG1168526.1"
                     /translation="MSYSLNWNLDSIFSGGSHSDALNQRMKQLEDQMNEYYHRVTKWS
                     PSSDNAEQLNAILQLQETITNGFTQCSSYITALLSANVNDSDAKVLSGKLYAMLPRLQ
                     SAETVLSKKFAEISDNDWNQMLSQSSFETIAFRLNEIRRDGSQLLSEAEENIINTLSL
                     DGLNAWSSHYDTIVASISIPFEQDGQVVELSAGQAFNKMMGDPDPKVRETLFAAWEEA
                     WKEKAPLFTDTLNHLDGFRLSDYELHGVTDFLQKPLEYNRLKKETLSVMWDTIQKNKQ
                     PLVNYLTRKANLFGKEKMEWQDQDAPIILGDLKEKTFSFDEAAAFIIENFNKFSPKMA
                     KFAQSAFEKSWIEAEDRPGKRPGGYCTELPETKESRIFMTYSNSVNEVATLAHELGHA
                     FHSSTMWDLPSLNREYAMNVAETASTFAELIVADATLKVAKTKEEKINLLDTKLQNAL
                     AMFMNIHSRFIFENRFYEARQEGLVSEDKITEMMVEAQKEGYQGALATYHPYFWAAKL
                     HFFIDDVPFYNFPYTFGYLFSLGIYAYANQKGANFEEEYIHLLRDTASMTTEDLAKKH
                     LNVDLTQPDFWQAGIDMVTEDIQTFMELTEEFI"
     gene            complement(21321..22895)
                     /locus_tag="SCJ86_000946"
     CDS             complement(21321..22895)
                     /locus_tag="SCJ86_000946"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287386.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="C40 family peptidase"
                     /protein_id="HEG1168527.1"
                     /translation="MKKSLISAVMVCSMTLTAVASPIAAAADDFDSQIQQQDQKIADL
                     KNQQADAQSQIDALESQVSEINTQAQDLLAKQDTLRQESAQLVKDIADLQERIEKRED
                     TIQKQAREAQVSNTSSNYIDAVLNADSLADAIGRVQAMTTMVKANNDLMEQQKQDKKA
                     VEDKKAENDAKLKELAENQAALESQKGDLLSKQADLNVLKTSLAAEQATAEDKKADLN
                     RQKAEAEAEQARIREQQRLAEQARQQAAQEKAEKEAREQAEAEAQATQASSTAQSSAT
                     EESSATQSSMTEESSSATQSSATEESTTPESSTEESTAPESSATEESTTAPESSATEE
                     STTVPESSATEESTTVPESSTTEESTTPAPTTPSTDQSVDTGNGTGSSTPAPTPTPTP
                     EQPKPVTPAPAPSGSVNGAAIVAEAYKYIGTPYVWGGKDPSGFDCSGFTRYVYMQVTG
                     RDIGGWTVPQESAGTKISVSQAKAGDLLFWGSQGGTYHVAIALGGGQYIHAPQPGESV
                     KVGSVQWFAPDFAVSM"
     gene            complement(23150..23659)
                     /gene="mreD"
                     /locus_tag="SCJ86_000947"
     CDS             complement(23150..23659)
                     /gene="mreD"
                     /locus_tag="SCJ86_000947"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373621.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein MreD"
                     /protein_id="HEG1168528.1"
                     /translation="MLKKETMKYYLPIVLFFLMLIDGHLTRMLGEWSKGTYMSNAHFL
                     ILALLCCSMAFEKRYLLITTIVLGAIYDAYYIGVIGIYAVALPLIVWLMYVMKDVIHV
                     NIFTEFFSMIIFVTGYELFTMVVQLIFKLAVVNNTYFITRFLGPTLLLNMIIFVLFIF
                     PFKKLFSDE"
     gene            complement(23663..24517)
                     /gene="mreC"
                     /locus_tag="SCJ86_000948"
     CDS             complement(23663..24517)
                     /gene="mreC"
                     /locus_tag="SCJ86_000948"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400920.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein MreC"
                     /protein_id="HEG1168529.1"
                     /translation="MKKFNPNKNIIITLVIVIIVVTILSVTAAQRANEGKPNFFQSIV
                     NDSVAMVDKTISAPVRWVRNGVDSVHNLFSTYAENERLKEKIDSYDDVSQQNKNYERE
                     IEALKKELELNQTLTSYEKVTANVITRSPDSWQDMLIVDKGSKDGIEANMAVMSQKGL
                     IGRVIEVNTASSKIELLSSSNESSNHFPVRVSSANGEAFGLLKNYDEKLHALVVTQLT
                     GDTDIKEGDVVQTSGLGGNSPANLPIGTVIKTKPDSYGLDREVYVKPYAEMYDVSVVT
                     IVQRLAED"
     gene            complement(24672..25310)
                     /locus_tag="SCJ86_000949"
     CDS             complement(24672..25310)
                     /locus_tag="SCJ86_000949"
                     /EC_number="2.7.6.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341868.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine diphosphokinase"
                     /protein_id="HEG1168530.1"
                     /translation="MKILLAAGGNPQNWPQSLLSKYDAYVGIDRGALYLLQAGYPVDL
                     AVGDFDSLSNEEQKEVFAKAKEYTRSPAEKDDTDTQLGILKTFERYPQAEVTLIGATG
                     GRLDHLLANLWLGMEPRFKPFAHQLIIRDCQNYLSYYLPGEHIVRQIPDMKYLAYCCL
                     TPVSNLTLKGSKYVLENKEVEYPTSYASNEFLSDTAEFSFSDGMIAVIQSKD"
     gene            complement(25311..25961)
                     /locus_tag="SCJ86_000950"
     CDS             complement(25311..25961)
                     /locus_tag="SCJ86_000950"
                     /EC_number="5.1.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629807.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribulose-phosphate 3-epimerase"
                     /protein_id="HEG1168531.1"
                     /translation="MKIAPSILSADFANLQRDIELVEKGGADYIHVDVMDGRFVPNIT
                     LGPNIVQAIRPVTKLPLDVHLMIVDPENYIPAFAKAGADIITVHVEATPHIHRALQMT
                     KSLGVKAGVVINPGTPVSMIKHVLPLADQVLVMTVNPGFGGQSFIEETVEKITELADL
                     RRENNWTYAIEVDGGIVPETAKICKDAGADVFVAGSYIYGAENPVGQIERLKEALD"
     gene            complement(25974..26873)
                     /gene="rsgA"
                     /locus_tag="SCJ86_000951"
     CDS             complement(25974..26873)
                     /gene="rsgA"
                     /locus_tag="SCJ86_000951"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738631.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome small subunit-dependent GTPase A"
                     /protein_id="HEG1168532.1"
                     /translation="MALIKGQIRKAISGFYYVYANGETYQTRGRGNFRNRKITPLVGD
                     QVIFESENQTDGYLLEILPRKNQLVRPPVTNVDLGIIVTSIVEPAFSYNLLDRFLVTL
                     EYESIEPIIFLTKTDLSDDPEKIEEIKQTYTKIGYPVIVPNHTGDTTELIRYFPERLS
                     VFMGQSGAGKSTLLNQISPELNLATGEISDALGRGRHTTRHVELIPIYGGLVADTPGF
                     SSIDFLTIEAGELPRQFPEFVEASVHCKFRECMHAKEPGCEVKHRVETGGIAQTRYDN
                     YLQFLQEIEKRKPMYAKKEGKRK"
     gene            complement(27038..29107)
                     /gene="pknB"
                     /locus_tag="SCJ86_000952"
     CDS             complement(27038..29107)
                     /gene="pknB"
                     /locus_tag="SCJ86_000952"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287377.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Stk1 family PASTA domain-containing Ser/Thr
                     kinase"
                     /protein_id="HEG1168533.1"
                     /translation="MIELGKKLNGRYQITGNIGSGGMANVFLAHDLILDRDVAVKVLR
                     FDFQNDQTAIRRFQREALAATELVHPNIVSVYDVGEEDNMQYLVMEYVKGMDLKRYIQ
                     THYPVPYETAVNIMQQILSAISLAHNHQIIHRDLKPQNVLIDNEGVVKITDFGIAIAL
                     SETSITQTNTMLGSVHYLSPEQARGSMATKQSDIYALGIILYEMLTGSVPFDGESAVT
                     IALKHFQDDLPSIKALDPNVPQALENVILRATAKEPADRYKSAEEMSDDLSTVLSPAR
                     ANEEKWQPHVMDNETKVITPLTEDAPMPDSFKSMPLPKDKPENETLPEEEEVLPDPKK
                     KGQKKWWLILLAILAVLGIGTAVFFASGGRGEVVVPDVSDLSEASARETLAKAKLEVA
                     TKTEEIADDKIEEGNVVKTDPAAGTTVKQKREVTLYISSGTKKIKLDDYSGKSYEDAE
                     ASLKELGFSADLIQKEEEFSSEVESGMIISQSPSEDTEVDPKTDTITFTVSKGPETVT
                     LSSHVGENYETVRNWLVTQGFNINNISATYDYSDTVPSGSIISQDPASGEVVAEETYI
                     NFVVSQGTEPKLNDISGYTKSEAQSYLASVGAEYIGHETSEYSNTVDKDKVIRTVPGA
                     GTTITKGMVVNVIYSKGPDPASSSSEKDSSDSSSSSSSSSSTSNSSKEESSSSTSSSA
                     STSESSK"
     gene            complement(29104..29844)
                     /locus_tag="SCJ86_000953"
     CDS             complement(29104..29844)
                     /locus_tag="SCJ86_000953"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002372072.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Stp1/IreP family PP2C-type Ser/Thr phosphatase"
                     /protein_id="HEG1168534.1"
                     /translation="MQIEYQSDVGRRRNTNQDYASVFTSRSGLKLAVLADGMGGHRAG
                     DIASQMAVTNLGEAWEKQEAVDDEKIAQWFIQSIQEENMLIYQRGQEQPEYNGMGTTI
                     VAAALSDDWFTIANVGDSRAYLIREDKIMQLTEDHSLVNELVKSGEISEEMAVNHPRK
                     NILTRSVGMPGTVEVDVSTHLWQLKDRLLLCSDGLTNMLSEEEIGTIINQEGPLSHKV
                     TELIDRANEAGGADNITVLLIEYKEDSV"
     gene            complement(29857..31215)
                     /gene="rsmB"
                     /locus_tag="SCJ86_000954"
     CDS             complement(29857..31215)
                     /gene="rsmB"
                     /locus_tag="SCJ86_000954"
                     /EC_number="2.1.1.176"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287374.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (cytosine(967)-C(5))-methyltransferase
                     RsmB"
                     /protein_id="HEG1168535.1"
                     /translation="MKKIPKKMKKSVRYMALLTLERVNKGGAYSNLLLNEMIKQSELN
                     QKDVGLFTELIYGTISHQRLLEFYVAPLIAKAKKVDDWVKMLLYLSVYQLEFLDKVPA
                     HAILNEAVEIAKVKGNPGTGKFVNGVLRNYQRQGAPDLQGIADPIERLSVKISLPLWM
                     TKRLVDQIGYEETEKLGLSLYQPSHASARVDTRRLSRDQAITVLQEEEITAEKSQVSP
                     YGIVAKKGHLASSSLFHDGVMTIQDESSMLVAPSMQIEPEHHVLDACAAPGGKTTHIA
                     SFLDPSCGGEVVALDVHEHKVKLIEDNAARLGVSSAVYAQKMDAREVSKQFEAEQFDR
                     ILVDAPCSGLGLLRRKPDIRYKKQPDELANLPKIQLEILESCAPTLKSSGILTYSTCT
                     ILKEENQDVVSAFLERHPDFERIDVLTNPMLQSSIEEKMLTLYPHQFYTDGFFICCLR
                     KK"
     gene            complement(31215..32153)
                     /locus_tag="SCJ86_000955"
     CDS             complement(31215..32153)
                     /locus_tag="SCJ86_000955"
                     /EC_number="2.1.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287372.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methionyl-tRNA formyltransferase"
                     /protein_id="HEG1168536.1"
                     /translation="MTKLVFMGTPAFSVPILEGLIESGYEIQAVVTQPDRPVGRKRVI
                     TPTPVKKAALKHGIRVLQPEKISGSPEMEEIIELVPDVIVTAAFGQFLPEKLLQVPKL
                     GAINVHASLLPKYRGGAPVHYAIMNGEKETGVTIMEMIKKMDAGGIYAQESIPITKQD
                     DVGTMFEKLSLLGRKLLLETLPNILDGQKPVPQDESEATFSPNITREQEAIDWHKTAE
                     EIDNQVRGMRPWPIAFTTYEGIRWKLLSVEVLAETTDKAPGTIIRKDKKNLWIACGKQ
                     TVLAINELQPAGKGKQAVHDFLNGSGQQVQIGQQVK"
     gene            complement(32146..32637)
                     /gene="def"
                     /locus_tag="SCJ86_000956"
     CDS             complement(32146..32637)
                     /gene="def"
                     /locus_tag="SCJ86_000956"
                     /EC_number="3.5.1.88"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287369.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide deformylase"
                     /protein_id="HEG1168537.1"
                     /translation="MRYPILIHPNDKLKRTAQPIDVITDETIALLDNLYETMIANDGI
                     GIAAPQVGQNKRIAVIEVDEGEKFELINPEIIEAKGESLDVEGCLSIPHVYGTVKRAD
                     EVTVRYYDRDGEEIEVTAFGYLARAFQHEIDHLDGILFIEKMIQQIPEEELEEYMEEH
                     LDD"
     gene            complement(32652..35060)
                     /gene="priA"
                     /locus_tag="SCJ86_000957"
     CDS             complement(32652..35060)
                     /gene="priA"
                     /locus_tag="SCJ86_000957"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307710.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="primosomal protein N'"
                     /protein_id="HEG1168538.1"
                     /translation="MTLTAEVIVDVPTMQTDQPFTYLVPSEMETALQVGMRVEVPFGN
                     GNRHVQGFVMAIQKSEESANPSLKAVIRLLDLAPVVNEELLSLADYMKKITYAFKITC
                     LQTMLPSVMKAEYDKLIYPLADTPEVQVLFDQREVISWKEAEEEGSLSQLIRWRQEQL
                     VDIKYEVHTRNKVKTIRLVRSLLTEKQIEEEWAKLRQNAKKQKELLLCLSELSQEEPI
                     AYFKNKGISTAVLNQGKEKGWLEFIESERYRDPYKDRVFDQTTALELNAEQKNAVEQI
                     ITAGQQQKDQVFLLEGITGSGKTEVYLQAIADVLSENKTAIMLVPEIALTPQMVERFK
                     GRFGESVAVLHSGLSQGEKYDEWRKIEREEAQVVVGARSAIFAPLKNIGLIIIDEEHE
                     SSYKQDETPRYHARDLAIWRSKYHHCPIVLGSATPSLESRARAQKGVYQLLQLNHRAK
                     AAAQLPAIEIVDMREEFQNHRTSTFSANLQEKIQNRLDKKEQTVLLLNRRGYSSFVMC
                     RDCGFVLPCPNCDISLTLHMDTRSMRCHYCGHEEPIPNRCPNCGGNKIRYYGTGTQKV
                     EEELRELYPQARILRMDVDTTRRKGAHEQILQKFGAKEADILLGTQMIAKGLDFPEVT
                     LVGVLNADTSLNLPDFRSSEHTFQLLTQVSGRAGRAEKAGEVVIQTFNPQHYAIELAK
                     KQNYEQFYQQEMHVRHRGGYPPYYFTVKITASHPEEQVAAKKIFQIANQLKEVLSPQS
                     LLLGPTPSMILRVKNRYYYQLIIKYKHEPNLPHVLDEILNGSQKEQRQGLFVAIDNEP
                     LNFI"
     gene            complement(35313..35618)
                     /locus_tag="SCJ86_000958"
     CDS             complement(35313..35618)
                     /locus_tag="SCJ86_000958"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287365.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit omega"
                     /protein_id="HEG1168539.1"
                     /translation="MMLKPSIDSLLDRVNSKYSLVILASKRAHELDASAQPTLDSFDS
                     VKSVGQALEEIDAGNVVNDPHPELKRERLKMEEEERQAQKEREQHELESRIREEQKM"
     gene            complement(35623..36237)
                     /gene="gmk"
                     /locus_tag="SCJ86_000959"
     CDS             complement(35623..36237)
                     /gene="gmk"
                     /locus_tag="SCJ86_000959"
                     /EC_number="2.7.4.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010766465.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="guanylate kinase"
                     /protein_id="HEG1168540.1"
                     /translation="MSERGLLIVLSGPSGVGKGTVRKAIFESEDNDFQYSISMTTRKM
                     REGEVEGVDYFFRSKEEFESMIEAGEMLEYAEYVGNYYGTPLSYVNKTLDEGKDVFLE
                     IEVQGAKQVKEKVPDGVFIFLTPPDLAELKSRIVGRGTDADEVIEKRMKVAKEEIEMM
                     ALYDYAVVNDEVPLAVERIKNIIASEHFRVDRVIGKYIKMLKEM"
     gene            complement(36724..37008)
                     /locus_tag="SCJ86_000960"
     CDS             complement(36724..37008)
                     /locus_tag="SCJ86_000960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287356.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEG1168541.1"
                     /translation="MKYYDVTFHELSGKTVVKRDIPSEKNGFDVWKDACADYNENELF
                     ILINDGAYVTMNRKFIVRIDTEEVEDPTEKARSRKDEIMGVVNTLSNMGF"
     gene            complement(37026..37916)
                     /locus_tag="SCJ86_000961"
     CDS             complement(37026..37916)
                     /locus_tag="SCJ86_000961"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YicC family protein"
                     /protein_id="HEG1168542.1"
                     /translation="MKSMTGFGKAIRETKAYQLDIEIKSVNQRFLDVQIRSPKILNFV
                     ENDFRQLIKQQLSRGRIEVFVNLAYIGKNEKQIVINWELIDSLITQLTDGIQQRYGSQ
                     NQVSISRMAERFVLDENFVTVEEKPEESMEELAQLALDTLKSALDSIEASREKEGQAL
                     ALVLKKNTEEFKEVLKKLDSFVEIYEKEFQIRYQKKLEEFLGAKVDEQRLLTELAILL
                     ERGDIHEELDRLAIHVQKLDELIEAQCPVGRELDFLIQEMNREINTIGSKSNAIEIKN
                     QVVQSKTILEKIREQVQNIE"
     gene            complement(37936..38154)
                     /locus_tag="SCJ86_000962"
     CDS             complement(37936..38154)
                     /locus_tag="SCJ86_000962"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875139.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdbC family protein"
                     /protein_id="HEG1168543.1"
                     /translation="MAQEFSYEIVEEIAILSENNKGWRKELNLVSWNGRPPKFDLRDW
                     APDHEKMGKGLTLTNEEFEQLQKAIENM"
     gene            complement(38124..39824)
                     /locus_tag="SCJ86_000963"
     CDS             complement(38124..39824)
                     /locus_tag="SCJ86_000963"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="patatin-like phospholipase family protein"
                     /protein_id="HEG1168544.1"
                     /translation="MEIKKVYFSQKMSLYSPIQQAKQPLPFFQSHQESRDAFWRSAKK
                     QCAFKAVSKKYTVYFTIAKSRGDVIFNNLLIEGETFEWKKFFRYMEACARFFIKENCC
                     FLFQSPLSEEWLRIFHKNGYQGTTQLNKKLVYHTALVLGGGGAHGAYQIGVWQALKEH
                     DINFEIITGTSVGALNGALILQGDMKKAVNLWKKLSTRQVLALPEMAAVEDLRERFYR
                     ERRQMTKTALIEGGVSSAPLEKMVKDNLDLSLLKHNPKIRLYTVSTKLPELAEVVTDI
                     QQTKTEEIPDWILASASFYPAMAYRKIGKNKYADGGYRNKIPIDIAINKGATEAFVVD
                     VQGPGPAKKIRMPDIFIHWKCQTLWTLGSFLLFDSQRNQLNLQLGYLEMKKRLGYYYG
                     NWYTFHSVKPARTYWRGFLSYLTKEIQLELSFFKSIKFWQKLRNLYKNRVVPETCGLA
                     MLELLAKKHFLLPNEIYQVDMMIQMICQQDIKSMPDDLLAQIGQLSTEEWRRYRKYQQ
                     KIQNERTKTVYFDYLLQGKRKDRLQHELLKQPVDTLLILYLYYLKEEQPWHKNFHMKS
                     "
     gene            40091..40864
                     /locus_tag="SCJ86_000964"
     CDS             40091..40864
                     /locus_tag="SCJ86_000964"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324467.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEG1168545.1"
                     /translation="MTESVYVHIDTTSNAVLTKGLTATDFANSIVHFPQNLLLLDPSA
                     SAGEYESHTGLKVIRGTENIQRFFSSSRNRRGFDELKWIDFTDLTMLKELTPLEISEL
                     LYFGHMKTHLHSPFFYKLQNNFVYFDLNDQLNRIYYRYLDEFYRIFASKLSSILSDKI
                     NVKKSFFKKETPVEKISVELLKEMRNLMQEGIIFSFDQVGLIDGKYRIPIYVIEDYIW
                     KLKGTRYREEEIIATLIYSVTDRHWQLVEDADLSYLPLK"
     gene            complement(40945..41514)
                     /gene="thiT"
                     /locus_tag="SCJ86_000965"
     CDS             complement(40945..41514)
                     /gene="thiT"
                     /locus_tag="SCJ86_000965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301201.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="energy-coupled thiamine transporter ThiT"
                     /protein_id="HEG1168546.1"
                     /translation="MGKNRVWVEGTIVAALAMVLSLIPIQIGSSFSISLGQIPLTLFA
                     LRRGTKAGMLAGFIWGILHFPLGQVYYLSVVQVLIEYPIAYTFAGAAGLMASNVQKGY
                     VDDNTKQIRKSIVFGALIGALSRYFWHFIAGVVFWGAYALWGMNPWLFSFVMNGASGL
                     STAIVTIIVLLGIQQTTPSIFVPDKRQEL"
     regulatory      complement(41580..41682)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00059"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="TPP riboswitch; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="thiamine pyrophosphate"
                     /db_xref="RFAM:RF00059"
     gene            complement(41688..42659)
                     /locus_tag="SCJ86_000966"
     CDS             complement(41688..42659)
                     /locus_tag="SCJ86_000966"
                     /EC_number="2.7.6.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016618611.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribose-phosphate diphosphokinase"
                     /protein_id="HEG1168547.1"
                     /translation="MSKHYFDPRLKIFALNSNRPLAEKIAAAVGVELGKSSVTQFSDG
                     EIQVNIEESIRGSHVYVIQSTSSPVNDNLMELLIMIDALKRASAKTINVVMPYYGYAR
                     QDRKARAREPITAKLVANMIQKAGATRMLTLDLHAVQIQGFFDIPVDHLMGAPLIANY
                     FLEKGIKGDDVVVVSPDHGGVTRARKLAEFLKSPIAIIDKRRPKANVAEVMNIIGQVK
                     GKTCVLIDDMIDTAGTITLAANALKEAGATSVYASCTHPVLSGPALQRIEDSAIERLV
                     VTDSIYLPEDRKIDKIDEVSVGGLMGDAIKRIHENKPVSPLFETKNK"
     gene            complement(42753..44126)
                     /gene="glmU"
                     /locus_tag="SCJ86_000967"
     CDS             complement(42753..44126)
                     /gene="glmU"
                     /locus_tag="SCJ86_000967"
                     /EC_number="2.3.1.157"
                     /EC_number="2.7.7.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346271.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional UDP-N-acetylglucosamine
                     diphosphorylase/glucosamine-1-phosphate
                     N-acetyltransferase GlmU"
                     /protein_id="HEG1168548.1"
                     /translation="MDARYAIILAAGKGTRMKSKLYKVLHPVSGKPMVEHIINRVSET
                     KPDEVITIVGHGAEQVKAQLGERSKYALQAEQLGTGHAVLQAASFLEGKKGTTLVISG
                     DTPLLTTETLNNLFEYHQGKNASATILTAQAENPTGYGRIIRDHIGIVEKIVEQKDAT
                     PEEALVQEINTGTYCFDNEALFDALSKVGTNNAQGEYYLTDIIEILKEEGHTVAAYQT
                     DDFEESMGVNDRIALAKANEIMRKRINQMHMVNGVSFVDSATTYIDAGVEIGPDTLIE
                     AGVQIQGNTVIGSDCVIGSHSKIVDSRIEDHVVIENSVIESSHVKKHADVGPYAHLRP
                     KAEIGENVHIGNFVEVKNAQIGKGTKVGHLTYVGDATLGEEINVGCGVVFVNYDGKNK
                     HHTTVGDHSFIGSSTNIIGPVEVAKNSSIAAGSTITDNIPEYALAIARARQVNKEGYA
                     KKLPYLN"
     gene            complement(44629..45447)
                     /gene="purR"
                     /locus_tag="SCJ86_000968"
     CDS             complement(44629..45447)
                     /gene="purR"
                     /locus_tag="SCJ86_000968"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875132.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pur operon repressor"
                     /protein_id="HEG1168549.1"
                     /translation="MKVRRSERLVDMTQYLLNHPHELISLTSFAERYESAKSSISEDL
                     GIIKKTFKERGYGTLETIPGAAGGVLFIPEISYEEAKKYIESLAERLSEQDRLLPGGY
                     VYLSDLLGEPELLRQVGRIIASKYLGEKIDAVMTVATKGVPIAQAVSYYLNAPFVIVR
                     RDSKITEGSTVSVNYVSGSSERIEKMELSKRSLKRGSRVLVVDDFMKGGGTVNGMKSL
                     IEEFESELVGITVFAESKFNGHRAIDDYTSLLYVKDVDTQTKNITVVPGNYFSE"
BASE COUNT        12404 a         9876 c         8237 g        15011 t
ORIGIN      
        1 aattccttag acagctttta ttcgattatc tcattgttgg gaatagcaaa acatcccgaa
       61 ttgattgtgc atcagttagc aacattacta atctgtcgat cccgatgcct agaccaccag
      121 ttggcggcat accgtattct aaggcttcca agaagtcttc gtctacaccg tgagcttcgt
      181 catttcctag ttcacgttct ttttcttgcg cttcgaatct ttctctttga tcaattggat
      241 cgttcagttc tgtaaaggca ttagcaaatt cgcgccccac tatgaatagt tcgaatcgat
      301 ctgtgaatcg aggatcttct ggatttttct tcgctaaagg cgaaacttcg actggatgtc
      361 cgtatacaaa cgtcggttgt gtcaatgttt cttcaacaaa tgtttcaaag aattcattga
      421 taacatgtcc gacacccata gcttcggtta cttctacatt gtgtttttcg gctaaagcta
      481 atgcttcttc caatgtcatt tcttgccaga agtctacacc agtttgttct ttgattgcat
      541 ctaccatatg gatgcgttta aagtcacttt ctaagtcgac agcttgtcca tcatatgtga
      601 tttttgctgt tcctagtact ttttcagctg cgttacggat aataccttct gtcagattca
      661 ttacatcatt gaaatcagta tatgctgtgt atgcttctaa catcgtaaat tctggattat
      721 gtgtagtatc aattccttca ttacggaaaa cgcggccgat ttcataaact ttttccattc
      781 cgccaacaat tagtcgtttt aaatgaagtt ctagcgcaat acgcaaatat aaatcaatgt
      841 ccagtgcatt atgatgcgtg ataaatgggc gtgcggcagc tccacctgcc tcattgtgca
      901 atactggtgt ttctacttca atatagccat tgccatctaa atagcgacgg atttcgctga
      961 taatttggct tcgtttcata aatcggtcaa agctttcgcg attactgatt aaatccaagt
     1021 agcgttgtct gtagcgttgt tcaatattcg ttaagccatg gtatttatct ggcagtggac
     1081 gaagtgcttt tgacaaaatc gtgatttctg aagctttgat agaaacttcg cctgtgtctg
     1141 ttttcatgat ttgtccagtt acaccaaaga aatctccaag gtctgcatgt ttgaatactt
     1201 cataggcctc atcacctact tggtcttttc ttacataaat ttgaatttgg ccttcacgat
     1261 cttgtagatg cgcaaatcca gctttccctt ttcctcggcg agtcatcata cgaccagcta
     1321 cactagcggt caatcccatt tctgctagtt cttcttttgt tcttggatca aatagttcat
     1381 gcaattcttc tgagttgtgc gtgcgttcaa atcgttttcc aaatggatcg attccatctt
     1441 cacgcaattg ttccattttt tgacgacgta caagcatttg atcatttaaa tcttctactt
     1501 gatgttgttg ttcctgtgtc acaaaagttc ctccattctc atgttaatct ctttcctata
     1561 atgccaatga aacacaaaaa aagcaagccg aacttggaat ttcggcttga aaattcacga
     1621 aaaacttaga aatcacaacg tttgtttcaa tagtactttt tccgcttttt ctacaaattg
     1681 atccagcagg tcattgattt cttgctgttt ttctgtctga ttgattgcta ctttcacttt
     1741 cgctgctcga ggaatccctt ttaaataata agccgcatgt gttctgaatt ctctacaagc
     1801 tactttttct cctttgagat ctgctaagcg ttgtaaatga agtttagctg tagcaatttt
     1861 ttcggctggt gttggttcag gaatcagttc ccctgtttcg agataatgct gtgtgcggtg
     1921 aatcatccat ggattgccca gcgctgcacg accaatcatc acaccgtctg caccgacata
     1981 gtcaagcatc cattttgcat cttctggcgt tttgacatcc ccgttcccca taaatggaat
     2041 cgtcaaatgt tttttcactt ctttcaatac ttcccagtcc gcttttcctt catacatctg
     2101 aacccgtgta cgtccatgca tagcgatggc acttgctcca gctgattgtg ctgctaatgc
     2161 attttctaca gcaaagacgt gctcttcatc ccaaccaata cgcatcttga ttgttactgg
     2221 gatatttact gctgaagaaa cagcttggac catttcgtat actttctcag gatctaaaag
     2281 ccattttgct cctgcttctg ctttgattac tttatttact ggacagccca tattgatatc
     2341 tattatatcg gcagctgtgt tttcttcgac aaattgagca gcttcaacta gactatcttt
     2401 atttccgcca aatatctgta aacttaatgg atgttctgtt tcttcaatgt ggagcatctc
     2461 taaagttttt ttgtttctga aatgaatacc ttggtcactg atcatttcac atacgactaa
     2521 gcccgcacca aattctttga cagtgactcg aaatgctgaa tttgttattc cagccatcgg
     2581 tgcaacaacg acacgattag ggatttccac attgccaatt ttccatgtgt gttccactta
     2641 aatccctcct aaggcttctt tcttcaatcc atctaattct tcttcgctat acttgtattt
     2701 gttattacag aaagaacaaa ccgcttctgc accgtgatct tcgtctatca ttgcttggat
     2761 ttcttctgca cctaacgcga taattgcttc tgcaaatttt tcttttgaac agtcacattt
     2821 gaattgaaca ggcatttttt ccaaaatgtt caattcttct gtaccaagca atcctttcaa
     2881 tatttcttct ggtgtctgtc cttcatctaa gagggtagag attcttggtg tcgtttttaa
     2941 ttgttcttcg atcttgctga ttgtttcttc atcggcacct ggcatgattt ggatcatgaa
     3001 tcctcccgcc gtcttgatgc tgtcgtcagt attgaccaat acactcagtc caacagctga
     3061 agggatttgt tctgagatag caagatagta agtaaaatct tcagcgattt ctccagaaac
     3121 aatcggcgtt tgcccagaaa aaggttcttt taatcctaag tcttttgtga cagtaaacat
     3181 tccttgtgtt ccaactgctc cccgcacatc gattttacct tcactattta gaggaagact
     3241 gagatgcgga tttttgatgt aacctttcac atctcccttt ccatttgcat ctactacaat
     3301 tcctgttgct ggaccatcgc cttggattcg aacagttagt ttgtcttctc ctttgagcgt
     3361 cgcaccaagt agcagactac cgaccaatgt ccgtcccaaa gcagctgagg aggtgtgcca
     3421 agtatcatga cgtctttgtg cctccgctac ggtatttgtt gcttgaacgg cgtatgctcg
     3481 gacgaatccg tcgtgagcaa gtgccttaac caaataatct ttcatttgtt atgtactcct
     3541 ttttccgttt ttaaactcat agtgaatcat actagcaact actgcttttt tcaactttgg
     3601 aatgcttctt ttaaaaataa ttcagaatca tctagaaaac aggacatcac cctatttaca
     3661 acttaaataa agaaatctcc cgaaaaccgg tttttgttcc ggttttcggg agtcatatca
     3721 gatcaattga cctatatttt tttatttgta ttgatcgtct gaatgattat cttctgattg
     3781 ttgagaatct gaatcatgtt ctgctttatt ttcctcattc agttcatgtt ttgcttcttc
     3841 aaaatcacgt ttttcttccg cttgtttttc agcgtctttt tcttccaaag cacgttttgc
     3901 ttcttcaaaa gttttcgctt ctttttcgct tggataatca tttgactcag taccttcagg
     3961 catcacaccg tgttcaaaca atgacttgat tgctttggca tccaatgttt cgaattccaa
     4021 aagtttttca gcaatcagtt tatgctgtgc acgatgagct tcgatgattt ctcgagcttt
     4081 atcatgagct tccatcaaga tttttcgtac ttcttgatca atctcgaaag cgacttgttc
     4141 cgagtaagct tttgtttgac catagtcgcg gccaacaaac acttgatggt ttccttcata
     4201 ttgaacagga cctaaacgat cactcattcc atattctgtt accatgcttc gtgctagagc
     4261 tgtcgcttgc tcaaagtcat tggaagcacc agttgattgg acattgaaga tgatttcttc
     4321 agctgtacgt ccaccaagta agcctacgat ttgttcaaac atatcttctt tcgtcatcaa
     4381 gaactgatct tcttttggta aagcgatcat atatccgccg gcgcgtccgc gagggatgat
     4441 cgttactttg tggacgaccc gagcgcgact caatactaat ccgacaatcg tatgaccagc
     4501 ttcgtgataa gcaaccattt cgcgttcttt tttgctgatt acgcgatctt ttttagctgg
     4561 acctgcaatc acgcgatctt ccgcttcatc gatatcagaa gcatcgattt tctttttatt
     4621 tcttctagca gctactaacg ctgcttcatt caagacattt tctaaatctg ctccggcaaa
     4681 acctggtgtc tgttgagcca caactttcaa gtcaacgtca tctgccatcg gtttattccg
     4741 tgcatggact cgcaagattg cttcacgacc ttttacatct ggacgtccaa ctaaaatctg
     4801 gcggtcaaaa cggcctggac gtagtaacgc tgggtctagt acatcagaac ggttggttgc
     4861 agcaatgacg attactcctt catttccgtc gaagccatcc atttctacaa gcaattggtt
     4921 caatgtttgt tcgcgttcat catgtccgcc gcccataccg gcaccacgtt gacgacctac
     4981 cgcatcaatt tcatcaatga aaatgatagc tggtgcgttt ttcttcgctg tttcaaacaa
     5041 atcacgaaca cgacttgctc caacaccgac aaacatttct acgaaatcag aacccgagat
     5101 cgagtaaaac ggtactccag cttctccggc aacagctttt gctaataagg ttttacctgt
     5161 tcctggaggt ccttctaaaa gtacacctgc cgggatacgg gcacctaatt caacaaatcg
     5221 gcgaggatct ttcagaaatt caacaacttc aactagttct tgtttttctt cttccgcacc
     5281 cgctacatca gaaaaacgta cgcgattagc ttttttgtct gcttctttgg cttttgattt
     5341 accaaagttc atgacacggc cgccaccgcc accgccgccg ccttgttgtc ccatcatcat
     5401 gtaaaagaaa aagatgatga ttacgattgg caagaagcta agcaataggg aaacccatat
     5461 accgctgctt gattcttctt ttactgttgt agcaactttg ttttcttttg ccagttctgt
     5521 tacttggctc aatgttgtat catttggcaa aatgattgtt gtgaaatggg tagtagtcgt
     5581 atctgttgaa cctaagatag ctaacccacc gctgttagcg acggtctgct gttctttgta
     5641 ttcaccagtg atcttatata caccatttgc aggctgcact tcaaattctt tgatcttgcc
     5701 tgcttgtagt tgttctgtga attttgagta ctcgatatct gacgatcgct ggccgttatt
     5761 accaaaaatg aaataaacga ccattaccat cgctaagatt acgaggacat aatacaaggc
     5821 atttttcatc atgccgttgt tctttttgtt catagttgcc ctccctaagc gtcaatttaa
     5881 tttaacagtt ttttatcaat cctgaccatt ctattgtacc ataatttaat tgagatacag
     5941 cattaatttg attgataaac actaggtttc aatacaccga tgtacggaag gttacgatat
     6001 gcttctgcat aatccaagcc ataaccgacc acaaattcat ttggtacgtt gaagccaaca
     6061 taatctgctt cgatatttac tactcttcct tcaggtttgt caagtagtgt aacaattttg
     6121 actgattttg ctttgcggta tttgaaaaga tccactaaat aagcaagtgt tcttccgctg
     6181 tcaatgatat cttcaacgat caacagatcg cgaccttcta cattcgtatc taagtctttg
     6241 acgattttta cttcccctga cgagactgtt gcattcccgt agcttgaaac atccatgaaa
     6301 tccaattcca aatacgtatc gatagaacga acgatatctg ccatgaatgg taccgctcct
     6361 tttaagacac ctactactag gggattggtg ttttggtaaa tttctgttaa ctctttgcct
     6421 agttctttgc aacgaacttg tatctcttct tgtgagatca aaattctctc aatatccttt
     6481 tccaacatca ggttctcctt ccaattgctt ctttttgata tttgtaaaga agtatgtagt
     6541 gtattttatc agtttcttca cgaatactca aatatgaaaa ggcaaaaggt atcaagctat
     6601 atatatttct tttggcatct gtaatgatcc aagcatgctg cctttgtgaa ttaggtactt
     6661 tctgatcgat aaaatatcta gatacttttt ttgtgagatt ctctgttaat ttaatgcggt
     6721 ctcctgcttt tcttttttcg acatacagtt gcacatcagc aggaatccaa atgtcatgag
     6781 aaaatagaga ccatgaatct gttgctttga tgtcccctgc attttcaggt ttgaccagac
     6841 caatccattc tgtttcattc aaatatattc cttgttctgg aatcagcaac aagcgctggt
     6901 cttctgtctg gctagttgct tgtttttctt ttttagagag tacaacaaaa tgatactctc
     6961 ttttcagtat ccaattattg ccaatactga gttgccattg gctttttttc tgttgccact
     7021 ggtctaaaat cgactccatt tgttgttctt tcaaaataat tcctgttttt gagaatgctt
     7081 tgtcgaaaaa ggagtaaaaa aaatagtatc gttcagcatc tgtcatttct tcaatcattt
     7141 ctgcagaaaa tcgaaattgt tcctcccact gttcgacttc cttttcgatc aactgtccca
     7201 tatatttttg aatgacttgg tctgcccatt ggatctgttg cgaaaattgc tggaaatgtc
     7261 tcaacgcctg tgtattttct tgtttcaatt gaggaactac caaatgacga agccgatttc
     7321 tctggatatc taacaattgg tttgtctgat cctcaaagta aacaagctgc gattccgctg
     7381 cataagtata taggtcttct ttgctaaaac tcagcaacgg acgaatcagt ctgcccgtag
     7441 caaatggttg tacttctttg attccagaaa aagtatttaa ctgtccccca cgaacgattt
     7501 tcattaacac cgtctcgatt tgatcatctc cgtgatgagc agtcatcaac gtgtcatatt
     7561 gtccttcttc catcacgcga gtgaattctt gataacgaaa gttacgagct tctgtttcta
     7621 tggcggattt ggctggatct tcccaaatac ttacatataa agggagattc ttctcttcac
     7681 aatatgagcg aagataggca gcttcttctg ctgattcttc tcttaattgg tgattgatgt
     7741 ggacgatacc taaagaaaaa ccgatttggc gttgcacttg taaaagtaaa tggagcaaaa
     7801 ccatcgaatc aacaccacct gataccgctg ctaaaattct cgatccttgt tgccagaatc
     7861 cttgttcatt cccgtgccga ataaatgact gctgtaatag cggtgctttt ttcccgcctt
     7921 tttcaacgtt catcctttcc acaccctttc ttcctaaaaa aaagagagcg ggaaaacgtc
     7981 taaaacgaca attaacctgc cctctgttga tactatgacc caaatagtcg cactatcatt
     8041 atttgcggct ttttagctgc gacgtccgcc acggccaccg cgctttcctt ctgtgtttcg
     8101 cttcaatgaa gataagcggt catcgctatc tttcaaaaat gaactcatca atgaatcgaa
     8161 gtcttctttt tgagctgaag gttgactgcg agaaaaagat tttttctgtg ctggacgatt
     8221 tcggttgtca cggggatctt tttgttgata ttttttagga cggctttctt ctccagattc
     8281 agcaggttgt tcctgcgctt tccggataga tagtccgatt tttccatcat catttactga
     8341 aataacttta actgttactt catctcctac acttaaaaca tcatggatgt cttttacata
     8401 gccatttgac acttcgctaa tatgaactag acccgttttt ccagcaccta gatcaataaa
     8461 cgcaccaaaa tttgtgattc ctgatacttt tccttgcagt tttgctccta cctcgattga
     8521 cataaaaaaa atgttcctcc taattatttc ccttttttat attcctgctt attttgcagt
     8581 tgtagaacta gttgattgat ctgtttcatt tgtcgagctg tctgtatttg attctaatat
     8641 aggataaact ttttcaccgt cttttgagta aaagaatttg cttctggcaa gtttagctac
     8701 atactcatca tctttcagca atgaaacatc ttggtgcagc tgatcaacgt ctgcgttgac
     8761 ttctttcatt tcagtatttg cttcgactct caattcgttc aattgatgca tcctttgcat
     8821 gtcatggaaa atctgcacac ctattatcgc gaaaatgacc aaagcaccaa caaaaattgc
     8881 agccagtctg cgtcttcgaa aaatcagttg acgttgttgt tgctgaaatt cagcatactt
     8941 tttctttgca tattccgtat ctagcgcagc gattttattg ctttttttcg tcacttggat
     9001 tcgctccaat ctttttgatt gtctttttca ttataccaat atcgtgaaag cttgtctaat
     9061 ctatttctct agtgtttctg tgcgattttc agaaataatt tcgtacattt tttgtgcatc
     9121 ttcttttttg gttgattcat gtaattccgt aattttcact tccaacgttt tgttgccaaa
     9181 ctggactttc aaaatatcgc ctactttgac atcactggaa gatttcgcca atttaccgtt
     9241 gatttgaatt cttcctttat cagctacttc ttttgctact gatcgtcttt tgatgattcg
     9301 ggaaattttt aaaaatttat ctaatcgcat atcttattcc tcacttttta atttttttat
     9361 ttaatcgcag gattttctta ccaaaaggca acattagcca ttctcgtact gtaaacagac
     9421 gaagacgaat cactgcataa atgaacacag aagccccgat cccaactcct atcacgctga
     9481 caaaaaaagc agtggaacga tgagttacag gaccaaatac tatattcaat acaccataat
     9541 acagcagcag tactactgtc atacaaagaa ggctgaacat caacttccgt ccaaaatgcc
     9601 gttctttcca aaaagcattt agctcttttg tttcaattcg aacaaaatac caaagcgtca
     9661 cacctaatcc taaaagggta gacaggcttg cacctgctgt tcctaataaa ccggtcagaa
     9721 aagaagtagt caatcctttt accaatagtc cccatcctgc gccttttaaa gaagggcgaa
     9781 acgagttctt gctttgagca atactttgat aagcttggat gactgcagtg aaagcaatgg
     9841 aaaaaacaaa taatacaagc gctgcatttc ccgcataatc tttaaacaac gcataattga
     9901 tataagggag cagcaaggct aatcccaaag acgcagctaa tgctaaagcg gtcgtcaatc
     9961 taagatagat tttggctgtc tgtaaaaact gacgatagat ccggttggtc aaatgacgag
    10021 tcaacgcagg caggaatgtc gcacttaatg cagtggcaat cactaaacct aattgtacta
    10081 aaggctgacc tcgatcatag accccttttg ctatcttcgc accgtattct gacagtccgt
    10141 aaacttccaa agcatttttt acaaagaaag agtcgatcag ctggaaaaag ataagcaatc
    10201 cactataaat cgaaaccagt ccgccttcta ccagaaaacg ccggatcaag ggctttgacg
    10261 gctgtttgaa caatggaaac cgtcggaagc ttaacgctcc tccatgaatc ttttgttcat
    10321 agtagtgaag gatcccatat gcgcataaac cgccaaaaac agctccactc attgctgccg
    10381 tccctgtttg gtacaccgtc catccaagct ttctaaaagc aaacgcacta cagacaatca
    10441 caaaaacacg aacgaactgc tcaactactt gagaaacagc agttggttcc atcaagaatc
    10501 ttccttggaa attccctcga taaaaagata gaccaggcat cagcaaaaag gtaaaggaaa
    10561 ctacttggat cagcggtttc aattgtatgt ctcccatcat ccaagcaatc cagctgctaa
    10621 atgcaaagac aaacaaccac aaaaagacac ctgtccaaaa tactaaggga taaagatttt
    10681 gcaaagcttg ctttcgctcg aaaggattct ttctttctgc aacgtacttt gatatgaact
    10741 gcggaagacc ggataaagct aaagtcattg ctatcccata aattggataa acttgctgat
    10801 aaacataaaa accttcgtcg ccgactaagt tttggagggg aattcggtaa agggcactta
    10861 atacttttgc tatgaaagaa gcaatcgtca atacaaatgc tccttgcatg acgcgccgca
    10921 tttctttatg atccaaatca ttgctccttc ctacgctgac agatattttt gctcacgcaa
    10981 cgctttgaca aacttttgga tttcctgaag ccatactgct tcttccattt ggttggggat
    11041 cgtcaagcgt atcaccatct gttctttttc aacgcctaga ctggctttta gttttgtcgc
    11101 tgtcagtgct tcgaataact gttccacact atagcgtttt gttccaactt tacttagagt
    11161 aaagactata tgcgcctcct gtttccttat cgtttctagg agcgctcgat cgccatccat
    11221 tttgatctgc ccaattgtca agagatgtgc tacttcttct ggatattctc caaaacggtc
    11281 gagcagatca tcttctagtt catctagcat ttcttgcgaa tcaagttcac gaattcgttt
    11341 atagatctcg attttctgcc gttgatctgc cacgtatgtt tcaggtagat acgcatcgac
    11401 accgaggtcg atctcaacag tggtcttctc cacttgacta ttctttcctt gtttacgaga
    11461 aacagcttcg cttagcattt gagaatacat atcgaaaccg actgcatcaa taaatccatg
    11521 ctgctgagca cctaagagat tacctgcacc gcgaatggaa agatcccgca tcgcaatctt
    11581 gaatccagat cccagttctg taaaatcttt gattgcctga agacgttttt cactgacttc
    11641 gttaaggatc ttctgctgct catacataaa gtaagcatat gcgacacggc tgcttcttcc
    11701 tacccgccct cgcaattggt ataacgtgga taatcccata taatcagcgt tctcaacgaa
    11761 caacgtattt gcgttcggga tatctactcc tgtttcaatg atggttgtag tgacaagaac
    11821 atcatattga ccttcaataa attcaaaaag cgtattttct aattgcgcct ctgtcatttg
    11881 cccatgagca taaccgatcc gtgcttccgg aacgagttct tggatttctt ctactttttg
    11941 ttcgatcgtt tctacccgat tatataaata gaatacttgt ccacctcgtc ccatttctcg
    12001 atggattgct tcgcgaatag ccccaggatt tttttccata acatacgttt ggatcggata
    12061 acgattcgca ggcggtgttt cgattactga aagatcccgc acgcctaaca tcgacatatg
    12121 caaagttctc ggaatcggtg ttgcagtcag agtcaaaaca tccacttgtg cacgtaactg
    12181 tttcaatcgt tccttatgtt ttacaccaaa acgttgttct tcgtcaatga tcagtagacc
    12241 caaatcgcta aattcaatat cttttgacaa aattcggtgg gttcctacaa caatatcaac
    12301 ttgacctgta cgcagttgtt cgatggtttc tttttgttgc tttcttgttc tgaaacgact
    12361 taatagcccg atatttacgg ggaaaccttc aaatctttcc agcatcgttt catagtgctg
    12421 ctgtgccaag atcgttgttg gaactaaaaa ggctacttgt ttgctttcct ttatcgcttt
    12481 aaaggccgct cgaagcgcta cttctgtttt cccataacca acatcaccta cgagtagtcg
    12541 atccattggt ttttcttttt ccatatcccg tttgatctcc gctgcacttc tcaactggtc
    12601 atcagtttca gagtagggga aggcattttc gaattctttt tggtaggcgt catcaggtcc
    12661 aaaagcatat cctttttctg actctcttgc ggcatataat ttgattagat cgtcagcgat
    12721 atcctcgatt tttgaagaga cttttcgctt ggttttcgtc cattcacttc cgcctagctt
    12781 attgatgcga ggcgctttgg cttcagaggc gacatatttt tggatcagat tcagttgagt
    12841 aacaggaata aacagtttgt cgtcgttttg atacaaaata gtcatatagt cttggtgaac
    12901 gccatctact tctagcgttt ccatcccaat gtatttcccg ataccatgat tggcatggac
    12961 aacgtaatct cctgctttta actcattata gcttttcaat cgctctgcgt ttgttactgt
    13021 ttgacgtctc gtttgttttt tcgtcgtttt ttggaaaatt tctttttctg tgatagctac
    13081 tattttttct tgaggaagtt caaaaccagc ctgcaaagct ccttcaacta attgtacctg
    13141 tcctctgatc aatgcatttt tttctgtttc tactactaat atgtcttcat ctcgaagcat
    13201 ttgttctgct ttgcgtattc tgtcctttgt cggaataaag atcacaacag tctgtttttg
    13261 cttgcgccaa cggtccattt ctgttttcag caatggcatt tgtccgaaga actgttgcat
    13321 cgtccgatat tgaaaattat ggattgcttg gaaacgaaga tttcccatcc ctttttggaa
    13381 gagtgagaaa aaggttgtgg tgaacttttc cttctgcacg attttttgta tatctgctga
    13441 aaatgtctgc tcagaaaaca cccgcaattc gctgattttt tgagtctgcc attctgccgc
    13501 ttccctttcg atttcccgat ttgtttccat gattcgctga taatcatcaa cgactagtaa
    13561 actatttgaa gggaaataat ccaagatcgt cacagattct tggtaaagaa gatctgagta
    13621 gaattttgct tcttctgtcg ggacacctgc ttgccagctt gaagtcagct gaccgaagta
    13681 atcttgcaaa aactcgcggt ctgcttcttc tgtggtcgca gcaagccgtt tttctagcat
    13741 tttttgcatt tgctctattc catgttctag atcctctggt ccgtagactt gttcactcgt
    13801 tggggaaacc cagatttctt ccgttttttc gattgagcgt tgtgtttctg cttcaaaata
    13861 ccgcatagaa tctatctcta catcaaatag ttctacacga acaggatagg ttgtcgttaa
    13921 cgggtaaata tcgataatgc ttcctcgaat actgaattct cccggttttg cgaccatctc
    13981 ttctcgtaca tagcccatca aagtcaattg gcgaggaaga ttttccacgt ctatctcatc
    14041 accaatatgc caattcaatt gcgcctgtag ccaagttttc ttattcggca gtcgtttgtg
    14101 caaagatgcg actggcaaca aatagatacc tgcttttttt tcttgaagtg cgttaagcgt
    14161 tgcaactctc tcgcctcgcg cttctggaga agaaaaggcc atttcagcag aaagcacttc
    14221 atccactgga aataaatgga tctgatcttg cggaatcaca tgttgcagat cttctgctaa
    14281 ctgattcgca taatacaaat ttggcatcag aataatcatg tttttatttg ttttcttaaa
    14341 cgctcgagca aatagaagtg ttttcgctga gcccgctaat cctgtgatta gttgtctagg
    14401 cattttttct tctaaattgc tgacccattc ttttacttgc ggatcttttc cgactaattg
    14461 aataatatcc attccttttc ctccttcctc aattgaatcg attcatcgca tccacgaacg
    14521 aatgcccttc tatgagatat tcgactgctt taacagattg acacatgctt tcttctatca
    14581 aaggctgatt ttctttgctg aaaggactaa gcacatgctg gactactgtt tttttccctt
    14641 ctggcctgcc gattcccacc ttgattcgat caaagacatt cgtattcaaa tgggaaataa
    14701 tacttttgat accgttatgc ccgccggcac ttcccttttg acggagccgg atttttccca
    14761 ctgccagatc caaatcatcg tagattacga ctaattcctc tggataaatc ccaaaataag
    14821 tcattagtgg gccgactgca cgaccagatt cattcatgaa tgtttgtggt ttgactaaaa
    14881 ggatcttttc tccattatga aaaaattcgg caacttctgc ttcgaacgga ttttttttga
    14941 aagtagcttg atgctcgcgt gcaattttat ccacgaccat aaagccgata ttgtgttttg
    15001 tatattggta tttggtacct gggtttccta acccgacgat cattttcatt tttgttttcc
    15061 acctaattct aaaaagcagc acaaaaggct ggactgctaa ttagcggtcc agtccatcga
    15121 gcttttttct tttttacact tgtcaatgca tagtataaca caaaacaagg cgtttcggct
    15181 aaaagagaat cgttaataaa tgataaagaa attaaaaatt tcttcaaagt ttaatgaaat
    15241 aattgatata taaaaatagt gaaataacgc acatgtaagc ttttacatat aatacaacct
    15301 tgttatctgt tatattttat agccaaatag cgtgacatgg ggcttgagaa atgttagcat
    15361 aagcatggga aaaaacaaat caatggaagg attgatacta aatgactgca gctgcagaaa
    15421 aaaaagatca tcaaaaagtt attcttgttg gagacggcgc cgtaggttct agctatgcct
    15481 ttgctctagt tactcaaaat atcgctcaag aagtaggtat tgtggatatc aatactgcta
    15541 aaactgaagg agacgcaatt gacttgtctc acgctttggc atttacttca cctaaaaaaa
    15601 tctattctgc tacttacgca gacgcacatg atgctgactt agttgttatt acagctggcg
    15661 caccacaaaa accaggtgaa actcgtttag acttagtaaa caaaaaccta aaaatcaacc
    15721 gcgatgttgt cacacaaatc gttgaatcag gatttaacgg tatcttttta gttgccgcta
    15781 acccagtaga tattttgact tactcaactt ggaaattctc aggtttccct aaagaacgcg
    15841 taatcggttc aggtacctct cttgactcag ctcgtttccg tcaagcactt gcggaattag
    15901 ttgacgttga tgcacgtaac gtccatgctt acatcttagg tgaacacgga gattcagaat
    15961 tcccagtttg gtcacatgca aatgttgccg gcttgcaaat ctatgaatgg gtaaaaaaca
    16021 acccagaaat cgatgaagaa gctatggtta acctattctt tggcgtacgt gacgctgctt
    16081 acacaattat cgagaaaaaa ggcgctactt tctatggtat cgcagtagca ttagcacgta
    16141 tcactcgtgc catcttagat gatgaaaatg ctgttcttcc tttatcagta ttcatgaatg
    16201 gcgaatatgg attgaacgac atctacattg gtgcacctgc tgttatcaac cgtcaaggta
    16261 tccaaaaagt aatcgaaatt cctttaagtg acagcgagca agaccgtatg gctgcttctg
    16321 ctaagcaatt aaaagaaatt ctagatgaag cttttgctaa actagacgct gaataatctt
    16381 tgaacatgac ggaaaacatc tattcggtgt tttccgtttt tttattttat cacccttatg
    16441 gtttctaaat tttttcataa agtagagata accttcgtct tttcctctaa attatgctat
    16501 catgagaccg ttccacccta aacttttact taaggagttg tcgatatgac aagatcttcg
    16561 catcgaaaaa aatcgaaaca tgttgctttg ctttcagttc ttggtgttct cttagtgctg
    16621 atcagctttt atacctatcg cagtacatac tacacaaagc attttttgcc gaatacagaa
    16681 ataaatggaa taaatgtcag caatctaaca gtcgaaaaag caaatgaaaa attaaaagaa
    16741 gcttattctg ataaaaaact ggctattaaa gaagatggaa aagtctggca ggaagtacca
    16801 aaatccgagc tcggctacaa agatgatttc acttcggagc tttctccgat cctaaatgaa
    16861 caaaacggat ggacttgggg aatgacttat gtctcagcgg cagaaaaaca agaaatagat
    16921 cctgtcagtc aaaaccacca aaaactagac actaccgttc agacattgac aactaagctg
    16981 actgatttga acaaagaccg gacacctact actgatgcaa cgatcgaaaa agcaggtgac
    17041 tctttcgtta tcaagcctga agtcaatgga aatacgatcg acgtcgatgc tgcggtcaaa
    17101 caattaaaaa gtgcggtaaa ctctggtaaa gatacaattg aattgactga attcaaagaa
    17161 aaaccaaaag taacatctga agacagctca ctgaaagaac aactcgcatc catgaatgct
    17221 atcgcaaatg taaaagctac ttacagtata aacggtgaga ctttccagat cccatcaagt
    17281 gatattatga gttggctgac ctacaatgat ggaaaagtcg atttagatac agaacaagta
    17341 cgtcaatatg taacagattt aggcacaaag tataacacaa gtacgaatga cacaaaattt
    17401 aaaagtacaa aaagaggaga agttacggtc ccagttggca cttacagttg gacgatccaa
    17461 acagacagtg agacagaagc attgaaaaaa gcgattctgg caggccaaga ttttactcgt
    17521 tcacccattg tgcaaggtgg cacaaccgct gatcatccat tgatcgaaga tacgtatatc
    17581 gaagttgatc tggagaacca gcatatgtgg tattacaaag acggaaaagt ggcccttgaa
    17641 acagatatcg tttccggaaa gccaactaca cctacacctg ctggagtctt ctatgtttgg
    17701 aataaagaag aagatgcaac tttaaaagga acaaatgacg atggaacacc ttacgagagt
    17761 ccggtaaact actggatgcc gattgactgg acaggtgttg gtatccacga ttcagactgg
    17821 caacctgaat acggtggaga cttatggaaa acccgcggtt cacacggctg catcaacacc
    17881 ccaccaagtg taatgaagga attattcggg atggtcgaaa aaggaacgcc tgtattggtc
    17941 ttctaacaaa aataatatat aaaaagaaag cacccgtacg attggtacag gtgctttctt
    18001 tttattatct aacaatgtgc tacttacatt tcagcagcag taaagacttc tgaaacatca
    18061 tcatcgtctt ctagcttatc tactagacgt tccaattgct ctttttgttc ctcatttaaa
    18121 tcaatcgtcg tttgtggaat catcgtcaat tctgcttgcg ccaaagtgaa ctctttttcc
    18181 aactcatctc gtacagcagc aaaatcttct ggtgcagtat aaatctcgaa tacttcagga
    18241 gaagtttgca gatcttctgc ccccgcctct aatacatcct caaacatcgt ctcttcatca
    18301 acagtcaacc catcgcgttc aatagcgata tatcctttgc ggtcaaacaa gtaactgact
    18361 gaacccgttt ctcccagtga tccaccgttt cgagtaaaag ctactcggac atttgtcgct
    18421 gtccgattac ggttatctgt caatgcatgg actaagattc ctactccacc cggaccatat
    18481 ccttcatacg tgatttcatc gtaattcgtt tcatctacgg cactcgtagc ctttttaatc
    18541 gcccgttcca cattgtcgtt aggcatattt gcagcttttg ctttgtccac aacaagccgc
    18601 aaagctggat tcatagatgg atcaggaccg cctgctttag ctgccatata aatttcacga
    18661 gaaagtttct gaaagacttt ccctcgtttt gcatcttggg cattttttct tccttggata
    18721 ttatgccatt tactatgtcc tgacatgatt tttcctccct tgttcttttg agaatacact
    18781 tctattctag tactatttca caaaaaaaca aagccacagc caatctataa acgcttcttg
    18841 aatgccgcaa aaagcacaca taatatcaca gcagtcagag caccgatcga atccaacatg
    18901 acatcttgga acataggtgt tcgtccaccg gtcatcatct gatggaattc gtccaaacct
    18961 gcgtaccctg ttgctgaaag ccaagcaagg atcgctgtca gccaccaaat ttttagcttg
    19021 gggaaaacac ctaaaaacaa gctgcctccc aaaataaaat aggtaccgaa atgtgcgccc
    19081 ttccttaaaa agaactctaa aaatttggcg tagcccagat ggttgatact caccatactt
    19141 ccaccataat taaaacgtat acccgctaat gtgtctttaa atggctgatt aggcagccag
    19201 ctttctaatc gcgaaacttg tgattgctgt ccgtaagttt gagaagaact gtaaaataag
    19261 attataacga ctatcagtgc gattactaaa taaatatttc cgtttttcat ttgttttttt
    19321 gaagaattca taagtcacct ctgtatttta tcttagactt tttttgaaga aacaacaatc
    19381 attttcatag agacttcttc cataaaaaag tgctatactt aattttgtat tacaatatga
    19441 agggagtttt ttcatgagtt attcattaaa ttggaatcta gattctattt tttccggagg
    19501 cagtcattct gatgctttga accaacggat gaagcaattg gaagaccaaa tgaacgagta
    19561 ttatcaccgc gtaacaaaat ggtcacctag ttctgacaat gcagagcagt taaacgctat
    19621 tcttcaattg caagaaacga ttacgaatgg ttttacccaa tgcagcagct atattactgc
    19681 attgctttct gccaatgtca atgattcaga tgcaaaggtc ttatcaggca agctttacgc
    19741 aatgctgcct cgtctgcaat cagccgaaac tgtattgtca aagaaatttg cagaaatatc
    19801 tgacaatgat tggaatcaga tgctttctca atcttcgttt gaaacaattg cttttcgttt
    19861 aaacgagatt cgtcgtgatg gctcacaatt attatcagaa gctgaagaaa acattataaa
    19921 tacgctttct ttagatggtc taaatgcttg gagcagtcat tatgatacga ttgtagccag
    19981 catttcgatt cccttcgaac aagatggaca agtagtcgaa ttatctgctg gacaagcctt
    20041 caataagatg atgggcgatc cagatccaaa agtcagagaa acacttttcg cagcatggga
    20101 agaagcctgg aaagaaaagg cacccctctt cacggatact ttgaatcatt tagatggttt
    20161 tagactttct gactatgaac ttcatggtgt cacagatttt ctacaaaaac cattagagta
    20221 caatcggttg aagaaagaaa cattgtctgt catgtgggat acaatccaga aaaacaaaca
    20281 accccttgtt aattacttga ctagaaaagc taatcttttc ggtaaagaaa aaatggaatg
    20341 gcaagatcaa gatgcaccta ttattttagg cgatttgaaa gaaaaaacct ttagttttga
    20401 cgaagcagca gcatttatta tcgaaaactt taacaaattc agtccaaaaa tggctaaatt
    20461 tgcccaatct gcttttgaaa agagctggat cgaagcagaa gatcgtcctg ggaaacgtcc
    20521 tggtggttat tgtactgagt taccagaaac aaaagaatca aggatcttta tgacctacag
    20581 taattctgtt aatgaggttg cgacacttgc tcatgaacta ggacatgctt tccatagcag
    20641 cacgatgtgg gatttacctt ctttaaatcg cgaatatgcc atgaatgtcg cagaaacagc
    20701 aagtacattc gcagaactga tcgtagccga tgctacatta aaagtcgcaa aaacaaaaga
    20761 agaaaaaatc aatttgctag atacaaaact gcaaaatgct ttagctatgt tcatgaatat
    20821 ccattcccga ttcatttttg aaaatcgatt ctatgaagca cggcaagaag gattagtctc
    20881 agaagataag atcacagaga tgatggttga ggcgcaaaaa gaaggctatc aaggagcttt
    20941 agctacttac catccatatt tctgggctgc aaaattgcat ttctttattg atgacgtacc
    21001 gttttataac ttcccataca catttggcta cctctttagt ttgggcatct atgcttacgc
    21061 caaccaaaag ggagcaaatt ttgaagaaga atatatccat ttactacgcg atactgcctc
    21121 aatgacgacg gaagatcttg cgaagaaaca tttgaatgtc gatcttaccc aaccagattt
    21181 ttggcaagca ggtatcgaca tggtgactga agatatccaa acgttcatgg aattgacaga
    21241 agaatttatc tgatttctat tttatcacaa taaaaaaaga gttagtccag aatgggctaa
    21301 ctctttttga ttaatcattc ttacatgctg acagcaaagt caggggcaaa ccattgaact
    21361 gaaccaactt ttacgctttc gcctggttga ggagcatgga tatattgtcc tccgcctaaa
    21421 gcaatcgcta cgtggtaagt tccgccttgt gacccccaga ataacaagtc tccagctttt
    21481 gcttgagaaa ctgaaatttt tgttccagct gattcttgag gaactgtcca accaccgatg
    21541 tcacgaccag ttacttgcat gtaaacatag cgtgtgaacc ctgagcagtc aaagccactt
    21601 ggatctttac cgccccaaac atatggagta ccaatatatt tgtaagcttc tgctacgatt
    21661 gctgcaccat tcacacttcc agaaggagct ggagcaggtg ttacaggttt tggttgttca
    21721 ggtgttggtg ttggcgttgg agccggagta ctacttcctg tgccgtttcc tgtatcaaca
    21781 ctttgatctg ttgatggtgt tgtaggcgca ggtgttgttg attcttctgt tgttgaagat
    21841 tcaggcactg ttgttgattc ttctgttgct gaagattcag gcactgttgt tgattcttct
    21901 gttgctgaag attcaggcgc tgttgttgat tcttctgttg ctgaagattc aggcgctgtt
    21961 gattcttctg ttgaagattc aggtgttgtt gattcttctg ttgctgaaga ttgtgtagct
    22021 gatgatgatt cttcagtcat tgaagattgt gtagctgatg attcttctgt tgctgagctt
    22081 tgtgcagtag atgaagcttg agtagcttgt gcttcagcct ctgcttgttc gcgtgcttct
    22141 ttttcagctt tttcttgtgc agcttgttga cgagcttgtt ctgctagacg ttgttgttca
    22201 cgaatacgag cttgctctgc ttcagcttct gctttttgac ggttcaagtc agctttcttg
    22261 tcttcagcag ttgcttgttc agcagctaat gaagttttca gtacgttcaa atcagcttgt
    22321 ttagataaaa gatcgccttt ttgagattcc aaagcagctt ggttttcagc taattctttt
    22381 agtttagcat cgttttcagc ttttttgtct tctacagctt ttttatcttg tttttgttgt
    22441 tccatcaagt cgttgtttgc ttttaccatc gttgtcattg cttgaacgcg tccaatagca
    22501 tctgctaatg aatcagcatt caatacagca tcgatgtagt ttgagcttgt gttgcttact
    22561 tgtgcttcgc gtgcttgttt ttggattgta tcttcacgtt tttcaatacg ttcttgtaaa
    22621 tctgcaatgt cttttactaa ttgtgcagat tcttgacgca atgtatcttg ttttgctaat
    22681 aggtcttgtg cttgtgtgtt gatttcagaa acttggcttt ctaaggcatc gatttgtgat
    22741 tgtgcatctg cttgttgatt tttcaagtct gcaatctttt gatcttgctg ttgtatctga
    22801 gaatcaaaat cgtccgccgc ggcagcgatc ggtgatgcta cggcagtcaa ggtcattgaa
    22861 cataccatta ctgctgatat taaactcttt ttcactcttc attcctccga ctggcttaat
    22921 ttaataaata tctaaatata tttaagtctt tcttaacgat ataaaaattg taccatagca
    22981 gtgtgtcaac gatataatag ttatgttaca aaaatgtttc aaaacaactt ttaaaaatga
    23041 caaaactgtt cgtttcagaa aatgatcatg aaaaagccgc gttaagtcta gcatttctta
    23101 agataacaac gattcttttt cgctttcgac ctaaaaataa aaaaagaaat tattcatcac
    23161 tgaataattt cttaaaggga aaaatgaata atacaaatat aatcatgttc aacagcagtg
    23221 taggtcctaa aaaccttgta ataaaatacg tgttatttac tactgctaat ttaaaaatca
    23281 actggaccac catcgtaaac aattcataac ccgtgacaaa gatgatcata ctgaaaaatt
    23341 cagtaaagat gttgacatgg ataacgtctt tcattacata catcaaccat acaattaaag
    23401 ggagagctac tgcatagata ccgataacgc caatatagta agcgtcatag atagccccga
    23461 gaacaatcgt ggtaatcagt aaataacgtt tttcaaacgc catactgcaa cataataatg
    23521 ccaatatcag aaagtgggca tttgacatat aggtgccttt cgaccactcc cctagcattc
    23581 ttgttaaatg accatctatc aacatcaaaa agaacaaaac gattggcaga tagtacttca
    23641 ttgtttcttt cttcaacatg gactaatcct ccgctagccg ttggacaatc gtcacaactg
    23701 acacgtcata catttctgca taaggtttca cataaacttc ccgatccagc ccataactat
    23761 ctggtttcgt tttaataacc gtaccgatcg gcaagttagc tggagaattc cctccaagac
    23821 cggatgtctg gacaacatcc ccttctttga tatccgtatc accagttaat tgggtcacca
    23881 ctaaggcatg gagcttttca tcatagtttt taagcaaacc aaacgcttcg ccattagccg
    23941 aagatacccg tactggaaaa tgattggagc tttcattaga ggatgacagt aattcgattt
    24001 tagacgaagc cgtattgacc tcgatcactc ggccaatcaa tcctttttgc gacatgactg
    24061 ccatatttgc ttcgataccg tctttagaac ccttgtctac gatcagcatg tcctgccaac
    24121 tgtcaggcga acgagtaatg acatttgccg ttactttttc gtaactagtc agcgtctgat
    24181 tcaactcaag ttcttttttc aacgcttcga tttctctctc gtaattcttg ttttgctgtg
    24241 aaacgtcatc atagctatcg attttttctt ttagccgctc attttcggca taagttgaaa
    24301 aaagattatg gacagaatct actccatttc gtacccaccg tactggggca gagattgttt
    24361 tatcaaccat tgcaacactg tcattaacga tggattggaa gaaattgggc tttccttcat
    24421 tcgctcgctg cgcagcggtc acacttagta tcgtcaccac gatgataaca ataaccaagg
    24481 taataataat atttttatta ggattgaact tcttcacaaa gacacctcga attaattaga
    24541 ctcttgtgtt caattttatc actaaatgcc gtgaaacaaa aggtaagttg ctcctcttaa
    24601 gctttttttt agcaaataat agattctgtc atttttagcc tgaaagacaa atttttttat
    24661 ttgtttatgg atcaatcttt gctttgaata actgcaatca taccatctga aaaagaaaat
    24721 tctgcggtat ctgaaagaaa ttcgttactt gcataagatg tcggatattc tacttcttta
    24781 ttttccaata catacttgct gccttttaat gtaagattcg agacgggagt caagcagcaa
    24841 taagctaaat acttcatatc tggtatctgt cttactatat gctctccagg caaataataa
    24901 gacaaataat tttgacaatc tctgataata agctgatggg caaagggttt gaagcgaggt
    24961 tccataccta accagagatt ggctaaaaga tgatccaatc ttccgcccgt agctccaatc
    25021 aaagtgactt ccgcttgcgg gtaacgttca aatgttttta agatacctaa ctgtgtatct
    25081 gtatcgtctt tttctgcagg ggaacgggta tactctttgg cttttgcaaa tacttccttc
    25141 tgctcttcgt tcgacaatga atcaaaatca cccactgcca gatctactgg gtagcctgct
    25201 tgaagcaggt acagcgcacc acggtctatc ccgacatagg catcgtattt ggacagcaga
    25261 gactgcggcc agttttgcgg attacctcca gcagctaata aaattttcat ttagtctaat
    25321 gcctctttta atcgttcgat ttgtccgact ggattttcgg ctccatagat atatgatcct
    25381 gcaacgaaga catccgcccc tgcatcttta cagatttttg ctgtttctgg aacgattccg
    25441 ccatctactt cgattgcata agtccaattg ttctctctac gtagatcagc gagttccgta
    25501 attttttcta ctgtttcttc aataaagctt tgtccgccga acccaggatt gacagtcatg
    25561 actagtactt gatcagccaa aggcaaaaca tgtttgatca tggagactgg tgttcctgga
    25621 ttgataacta ctccagcttt aactcctaag ctttttgtca tttgaagtgc ccgatgaata
    25681 tgaggagttg cttcgacgtg gacagtaatg atatctgctc cagcttttgc aaatgctgga
    25741 atgtagtttt ctggatcaac gatcatcaga tggacgtcta acggtaattt agtaacagga
    25801 cggattgcct gaacaatatt tggccctaat gtgatatttg ggacaaatcg gccatccatt
    25861 acatctacgt ggatgtaatc tgcgcctcct ttttccacta actcaatgtc tctttgtaaa
    25921 ttggcaaaat ctgcactcaa aatggatggt gctatcttca ttcttgtttc ctcctatttt
    25981 cgtttacctt ctttttttgc atacattggt tttctttttt cgatttcttg taagaattgc
    26041 aaataattgt cgtaacgtgt ctgtgcgata ccacctgttt ctactctatg ttttacttcg
    26101 cagccaggtt ctttagcatg catgcactct ctaaatttgc aatggacaga agcttctaca
    26161 aattcaggaa attgtctcgg cagttctcct gcttcaattg tcaaaaagtc aatcgagcta
    26221 aatcctggtg tatctgctac taacccgcca tagataggaa tcagttccac atgacgtgtt
    26281 gtatgccgtc ctcttccaag tgcatcagaa atctctcctg ttgctaaatt cagttccggt
    26341 gatatttgat tcagtagcgt cgattttcct gctccagact gacccataaa gaccgatagc
    26401 cgttctggga agtaacggat cagttctgtc gtatcacccg tatgattggg tacaatcacc
    26461 ggatagccta ttttagtata ggtttgcttg atctcttcga ttttttctgg gtcgtctgac
    26521 aagtcggtct ttgtcaaaaa aataatcggt tcgatcgact cgtattccaa ggtaactaga
    26581 aagcggtcaa gtagattata agaaaatgca ggctctacga tacttgtgac aatgattcca
    26641 agatcaacat ttgtgactgg tgggcgaact aattgatttt ttctcggcaa gatttccaga
    26701 agatagccgt ctgtttgatt ttcactctca aagatcacct gatctccgac aagcggcgtg
    26761 attttccgat tccggaaatt tcctcttcct cttgtttgat aggtctctcc gtttgcgtat
    26821 acatagtaaa aaccgctgat tgcttttctg atttgtcctt tgataagtgc cacctccgtt
    26881 tttgtttatt ttaccatacg aagaggaaag taggtgttat tccatgcaaa cttctcaaag
    26941 tatatggctt gaaaattgat caaaagagat agaaacacaa aaaagagaat gcaacaaatc
    27001 aatgtcgcat tcccttctga tatattttat tcagcactta ttttgaactt tctgaggtgc
    27061 tagctgatga acttgtggag ctggaagatt cttcttttga agagttgctt gtgctactgg
    27121 agctacttga gctgctagaa ctgtctgaag agtctttttc agaactagaa gacgctggat
    27181 ctggtccttt agagtaaatt acgttcacaa ccattccttt ggtaattgtc gttcctgccc
    27241 ctggtactgt acgaatcact ttatctttat ccaccgtgtt tgaatattcg gaagtctcat
    27301 gcccgatata ttctgcaccg acactagcta aataactttg tgcttcgctt tttgtatagc
    27361 cggagatatc attcaattta ggctctgttc cttggcttac gacaaaattg atgtaagttt
    27421 cttctgctac aacttcaccc gaggctggat cctgactgat gatcgaacct gatggtacag
    27481 tgtcgctata gtcataagta gcactaatat tattgatatt gaatccttgt gtaacaagcc
    27541 aattacgaac agtttcgtag ttttctccta catgactact caaagtcact gtttctggtc
    27601 ctttgctgac tgtgaaagta atcgtgtctg ttttaggatc gacttctgta tcttctgatg
    27661 gagattgcga aatgatcatt ccgctttcta cctctgaact gaattcctct tctttttgaa
    27721 tcaagtcagc agaaaaccct aattctttca aacttgcttc ggcatcttca taacttttac
    27781 cactgtaatc atctaatttg atttttttcg tgccgctact aatatacaga gtgacttcgc
    27841 gtttttgctt gactgtcgtt cccgctgccg ggtctgtctt cactacatta ccttcttcga
    27901 ttttgtcatc tgcaatttct tctgtttttg ttgctacttc taatttcgct tttgcgagtg
    27961 tttctctagc agaagcttca gaaagatcag aaacatcagg aacaacaact tctcctcgcc
    28021 cgccagaagc aaagaataca gctgttccga ttcctaaaac agcaagaata gctaatagga
    28081 tcagccacca ttttttctgt cctttctttt taggatcagg cagaacttct tcctcctctg
    28141 gtaaggtttc gttttctggc ttgtcttttg gcagaggcat agatttgaat gaatcaggca
    28201 ttggtgcatc ttccgtaagg ggtgtgataa cttttgtttc attatccatc acatgaggct
    28261 gccatttttc ttcgtttgca cgagctggtg ataatactgt agaaagatca tcagacattt
    28321 cctcggctga cttataacgg tctgcaggtt ctttagccgt tgcccgtaaa atcacgtttt
    28381 ccagtgcttg tggcacattt ggatcaagtg cttttataga tggaagatca tcttgaaaat
    28441 gttttaaagc aattgttaca gcagattcac catcaaacgg tacgctaccc gtcaacatct
    28501 cataaaggat gatccctaaa gcataaatat ctgactgctt cgttgccata cttcctctgg
    28561 cctgctcagg ggacagataa tgaacagatc ctagcatcgt attcgtctgc gtgatcgatg
    28621 tttcagataa agcgatagca atcccgaaat ccgtgatttt tacaacacct tcattatcga
    28681 tcagtacgtt ttggggtttc aaatctcggt ggatgatttg atggttatga gctaaagaaa
    28741 tagcagaaag gatctgctgc ataatattta ctgctgtttc ataaggaaca ggataatgtg
    28801 tttggatata tcgtttcaag tccattcctt tgacgtattc catcaccaga tactgcatgt
    28861 tgtcttcttc accaacatcg taaacactaa caatgtttgg atgaacgagt tctgttgctg
    28921 ctaaagcttc tctctgaaat cgtcgaatcg cagtctggtc attttgaaag tcaaatcgta
    28981 agactttaac tgctacatca cgatccaaga ttaaatcgtg cgccaaaaat acatttgcca
    29041 taccaccgct accgatattt ccagtgattt gataacggcc atttaatttt ttgcccaact
    29101 cgatcataca ctgtcctcct tatattcgat aagaagaacg gtgatattat ccgctccgcc
    29161 tgcttcattg gcacgatcaa tcagctcagt tactttatga gacaatgggc cttcttgatt
    29221 gatgatcgtt cctatttctt cttcagacag catgttcgtc aagccatctg aacataacag
    29281 cagtcgatct tttagttgcc aaagatgagt agaaacatct acttcgactg ttccaggcat
    29341 gccgacagaa cgtgttaaaa tatttttcct tggatggttc actgccattt cttcgctgat
    29401 ttctcctgat ttgactagtt cattgactaa agaatgatcc tccgtcaatt gcatgatttt
    29461 atcttcacga atcaaataag ctctgctatc cccaacattg gcaatcgtga accagtcatc
    29521 tgacagagcg gccgctacga ttgttgttcc cataccgtta tattccggct gttcttgtcc
    29581 acgctgataa atcagcatat tttcttcttg aatcgattga atgaaccatt gggcgatttt
    29641 ttcatcgtca acagcttctt gtttttccca agcttcgcct agattcgtta ccgccatctg
    29701 actggcaatg tctccggcac gatgaccacc catcccgtct gctaatacag caagcttgag
    29761 acctgaacgg ctggtaaata cactggcata gtcttgattt gtgttacgtc gtcttcctac
    29821 atctgattga tattcaatct gcattttttc acctcattac tttttgcgca aacaacaaat
    29881 gaaaaatcca tctgtgtaaa actgatgcgg atacaaagtc aacattttct cttcgattga
    29941 tgattgtagc ataggattgg tcaaaacatc aatcctctca aaatcagggt gtctttccaa
    30001 gaaagcagaa acaacgtctt ggttttcttc ttttaagatc gtacaagtgc tataagtcag
    30061 aataccggaa gactttaaag ttggtgcgca gctttctaaa atttccaatt ggatttttgg
    30121 taaattcgct aattcatccg gctgtttttt ataacgaata tctggttttc gccgaagcag
    30181 cccaagacct gaacaaggcg cgtctactaa aatccgatca aactgctctg cttcgaactg
    30241 tttactgact tctctagcat ccatcttttg agcgtagaca gcagaagaga cacctagacg
    30301 tgctgcatta tcttcaatga gtttgacttt gtgttcgtgt acatctaaag cgaccacttc
    30361 tccaccgcat gacggatcta aaaaagaagc aatatgcgtc gtcttacctc cgggtgctgc
    30421 acaggcatct aagacatgat gttcaggctc gatttgcata gacggcgcta caagcatcga
    30481 actttcatct tggatcgtca tgacgccatc gtggaacaat gagctagaag ctaaatgccc
    30541 ttttttcgct actataccgt atggactgac ttggcttttt tcagctgtga tctcttcttc
    30601 ttgcaaaaca gtgatggcct gatctctgga aagtcggcgt gtatctactc gggcactagc
    30661 atggctaggc tgatatagcg ataaaccgag cttttcagtt tcttcgtatc cgatttgatc
    30721 caccagtcgt ttcgtcatcc atagaggcag gctgattttg acagaaagtc tttcgatagg
    30781 atcagcaatg ccttgcagat ctggtgctcc ttgacgctga taattcctca agacaccatt
    30841 tacaaatttc cctgtcccag gatttccttt tacttttgcg atttcgaccg cctcgtttaa
    30901 aatagcatgt gcagggactt tatctaaaaa ttctaattgg tatacagaaa gataaagaag
    30961 catcttgacc cagtcatcca ctttttttgc tttagcgatc aatggtgcaa cgtaaaattc
    31021 caatagtcgc tgatggctaa tcgtaccata gatcaattct gtaaataaac ccacatcttt
    31081 ttgatttagc tcgctttgtt tgatcatctc attcaacaaa agattcgagt aggcgccacc
    31141 cttgttcact cgctctaatg tcagtaatgc catatatcgt actgattttt tcattttttt
    31201 agggattttt ttcattattt cacctgctga ccaatctgaa cttgctgacc gcttccgtta
    31261 aggaaatcat gtacggcctg ttttccttta ccagctggct gtaattcatt gatggctaag
    31321 acggtctgtt taccgcaagc aatccataga ttttttttgt ctttccggat gatcgtacca
    31381 ggtgctttgt ctgtagtttc tgccaatact tcaaccgata ataatttcca gcgtatgccc
    31441 tcataggtcg tgaatgcaat tggccaagga cgcattccgc gtacttggtt gtcaatttct
    31501 tctgcagttt tatgccaatc gattgcttct tgttcccgtg tgatgtttgg cgaaaaagtt
    31561 gcttctgact cgtcttgagg tactggtttt tgtccatcca aaatattggg caatgtttct
    31621 agcaatagct ttcttcctaa aaggctaagc ttttcaaaca tcgtccctac atcatcttgt
    31681 ttagtgattg gtatgctttc ttgcgcatag attccgcctg catccatttt tttgatcatt
    31741 tccatgatcg ttacaccagt ttctttctcc ccattcatga tggcataatg aacaggcgca
    31801 cctccccgat atttaggaag taaagaagca tggacattga ttgcccctaa ttttggtact
    31861 tgcaataact tttcaggcaa gaattggcca aatgccgcag tgacgatcac atcaggcacc
    31921 aattcaatga tttcttccat ttctggcgaa ccggagattt tctctggctg cagcacacgg
    31981 ataccgtgct tcagagccgc tttttttacc ggtgtcggcg taattactcg tttccgccca
    32041 actggacggt cgggctgtgt taccactgcc tggatctcgt aacctgattc tatcagccct
    32101 tcaagaatcg gcacagaaaa agcaggtgtg cccataaata ctagcttagt catcaagatg
    32161 ttcctccata tattcttcaa gttcttcttc cgggatctgc tgtatcattt tctcaatgaa
    32221 caagataccg tctaagtgat cgatttcatg ctggaaggca cgggctaaat agccaaaagc
    32281 cgtcacttct atctcttctc cgtctcgatc ataataacgc acggtcactt catctgctcg
    32341 cttgaccgtt ccatagacat gcggaatact gagacaacct tctacgtcaa ggctttcacc
    32401 ttttgcttcg atgatttctg ggttaatcag ttcaaatttc tcgccttcat ctacttcgat
    32461 cacagcaatt ctcttgtttt gtcctacttg aggagcagcg atccctattc cgtcattcgc
    32521 aatcattgtc tcatataaat tgtctaataa agcgattgtc tcgtccgtga ttacatcgat
    32581 tggctgagca gttcttttta atttatcatt agggtggatc aatataggat agcgcataat
    32641 ttggcccttt cttaaataaa gtttaatggt tcgttgtcaa ttgccacaaa aagaccttgt
    32701 cgctgttctt tctggcttcc attcaaaatt tcatccaaaa catgaggaag attcggttca
    32761 tgtttatatt tgatgattag ttgataataa tatcggtttt tcactctcaa aatcatgctg
    32821 ggcgttggcc ctaatagcaa gctttgaggg cttaacactt cttttagctg attggcaatc
    32881 tgaaagattt tctttgccgc tacttgttct tcaggatggc tggcggttat tttcacagta
    32941 aagtaataag gaggatagcc tccacgatgc cgtacatgca tttcctgctg ataaaactgc
    33001 tcataatttt gttttttagc caattctatg gcgtaatgtt gaggattgaa tgtttggatc
    33061 acgacttcgc ctgctttttc cgctcgccct gctcgtcctg aaacttgggt aagcaattgg
    33121 aatgtatgct cactagaacg aaaatccggt aaattcaggg aggtatcagc attcaacacc
    33181 ccaactaatg tcacttctgg aaagtccaaa cctttagcaa tcatttgtgt ccctaataat
    33241 atatctgctt cttttgcacc gaatttttgt aaaatctgtt cgtgtgcccc ttttcttcga
    33301 gtggtatcta catccatacg caaaatccta gcttgcggat aaagttctcg gagttcctct
    33361 tctacttttt gagtacctgt tccatagtag cggattttat tgccgccaca attaggacag
    33421 cgattgggaa taggttcttc atgtccgcaa taatggcagc gcattgaacg tgtatccata
    33481 tgcagtgtaa gagagatatc gcaattagga catggaagaa caaatccaca gtccctacac
    33541 atcacaaaag acgaatatcc tcgacgattg agcagtagga cagtttgttc ttttttgtct
    33601 aatcgatttt gtattttttc ctgcaaatta gcagaaaaag tagaggtccg atgattctga
    33661 aactcttcac gcatatctac gatctcaata gctggaagct gtgcagctgc tttggctcgg
    33721 tggtttaact gcaataactg ataaacgcct ttttgagctc gtgctcttga ttctaaagag
    33781 ggcgtcgcgc ttcctagaac tattgggcaa tgatgatatt tactgcgcca aattgctaga
    33841 tctctcgcat gataacgcgg tgtctcatct tgtttgtaac ttgattcatg ttcttcatcg
    33901 ataatgatca gcccgatatt tttcagcggg gcaaaaatag cagatcgtgc gcctactact
    33961 acttgcgctt cttcccgctc aatcttgcgc cattcatcat atttttcacc ttgggaaagt
    34021 ccgctatgta agaccgcaac tgattctccg aagcgtcctt tgaatcgctc gaccatttgc
    34081 ggagtcaatg cgatttcagg cactaacata atagccgttt tattttctga caaaacatca
    34141 gcgattgcct gaagatatac ttctgttttc ccgctgccag tgattccctc cagcaaaaag
    34201 acctgatctt tttgctgctg acctgctgtg atgatttgtt cgacagcatt tttttgttct
    34261 gcattcagtt ccaatgccgt cgtttggtca aatactcggt ctttataagg gtcgcgataa
    34321 cgttctgatt ctataaactc cagccagccc ttttcttttc cttgattcaa gacagcagta
    34381 ctgattcctt tatttttgaa ataagcaatt ggttcttctt ggctcagttc agataaacac
    34441 aaaagaagtt ctttttgttt cttggcattt tgccgtaatt ttgcccattc ttcttcgatt
    34501 tgcttttcag ttaacaaaga acggaccaaa cgaatcgttt ttactttatt tcgtgtatgg
    34561 acttcatatt tgatatctac taattgctct tggcgccagc gaatcaattg agaaaggctg
    34621 ccttcttctt ctgcttcctt ccaggaaatc acttctcgct gatcaaacaa tacctgaact
    34681 tctggtgtgt ctgccaaagg atagatcaat ttatcatatt ctgctttcat cacactcgga
    34741 agcatcgttt gtagacaagt gattttgaat gcgtaagtaa tttttttcat ataatcagct
    34801 aatgaaagta attcttcatt gaccaccgga gcaaggtcta gcaaacgaat aactgccttc
    34861 aaagatggat tagctgattc ttctgatttt tggatagcca taacaaatcc ttggacatga
    34921 cgattgccat taccaaaagg gacttctact cgcatcccga cttgcaaagc tgtttccatt
    34981 tcagagggaa ccaaataagt gaatggctga tcggtctgca tagtaggtac gtccacaatg
    35041 acttcagctg ttaatgtcat ggttcgtttc ctcctttttt tgtcttttct tatcaagcga
    35101 caaaaagttc cttgcctatt ttactagaga tcgatggatt tgtcttatct tctgccaaat
    35161 cctaacaaaa aattaacagc agataagaaa aaaaagaact gtgtcaaaaa gttcagacac
    35221 agccttttct accagacaat aagtggcgaa tgtttgaaaa ggaaacagct gggtttgctt
    35281 ttctttttag atgttcgctc tatttttctt tattacattt tttgctcttc acggatacgt
    35341 gattccaatt cgtgttgttc acgctctttt tgtgcttgac gttcctcttc ttccatcttc
    35401 aaacgttcac gttttaattc aggatgtgga tcattgacta catttcctgc atcgatttct
    35461 tctaaagctt gaccgacgct ttttactgaa tcaaaagaat ctagagtagg ctgtgcactt
    35521 gcatccaatt catgcgcgcg tttacttgct aaaatcacta gagaatattt tgaatttaca
    35581 cgatctaata gtgagtcaat tgaaggtttt aacatcataa gactacatct cctttaacat
    35641 tttaatatat ttaccgatta cgcgatctac tcgaaaatgt tcactggcaa taatgttttt
    35701 gattcgttca actgctagcg gcacttcatc atttacaaca gcatagtcgt aaagagccat
    35761 catttcgatt tcttcttttg caactttcat tcgtttttcg attacttcat ctgcatctgt
    35821 tcctcggcct acgatccgtg attttaattc cgctaaatct ggcggagtca aaaagatgaa
    35881 tacaccatcg ggcacttttt ctttgacttg ttttgctcct tggacttcaa tctccaagaa
    35941 aacatctttt ccttcatcca atgtcttgtt gacatatgac aaaggagttc cataataatt
    36001 cccgacatat tctgcgtatt ccagcatctc tccagcttcg atcattgatt caaactcttc
    36061 tttgcttcgg aagaaataat caacgccttc gacctcccct tcacgcattt tacgggtagt
    36121 cattgagatc gaatactgaa aatcattatc ctcactctca aaaattgctt ttcgtactgt
    36181 gccttttcct acaccggatg gaccagacaa cacgattaat aatccacgct ctgacatgac
    36241 gacgtccttt caactagtct ttttgtactt atcgtttatt ttgcactttt tttctgcaac
    36301 tttcaaggtt atctttaatt atagctagaa attggtaaaa aagctaccct gtaccaagag
    36361 gtcgtaaaaa agctgtcctg catcaagtaa taagaacagc caaacataaa cagcttctca
    36421 tgtttttcgt ttggttgggg ttattatcgc agatgcaagc ttttgaacgc cgtgtataag
    36481 aggtcgtgaa gaagccgatt agtttcgagt atgaagaaaa attttgtgaa aatagtttct
    36541 cacatttttg cataaatttg gcttaatacc gagaaaatgg tttttgaaca tcacttattc
    36601 aaaataaaaa aagattgtga cacgaatttt gtcacaacct ttaaatgtat tcctagagca
    36661 aagaagctaa gcgaagaaat ccaattctgt ttatttattg tatttctttg ttcagcatcc
    36721 ttgctaaaag cccatattgg acaaagtatt cactacaccc atgatctcat cttttcgaga
    36781 acgtgctttt tctgtcggat cttctacctc ttctgtgtca attcgaacaa taaatttccg
    36841 attcatcgtc acgtaagcac cgtcgttgat caaaataaac agttcgttct cattatagtc
    36901 tgcacaggca tctttccata cgtcaaagcc gtttttttca gaagggatat cccgtttaac
    36961 cacagttttt ccacttaatt cgtggaacgt tacgtcataa tatttcatta tctctacctc
    37021 tttcgtcatt caatattttg tacttgttct ctgatttttt ctaagatcgt cttagactgg
    37081 acgacttgat ttttaatttc gattgcattt gatttggaac cgatcgtgtt gatttcccga
    37141 ttcatttctt ggatcaggaa atctaattcc cgacctacag gacattgtgc ttcgataagt
    37201 tcatctagtt tttgtacatg gatcgccaga cgatccagtt cctcgtgaat atctccgcgt
    37261 tccaacaaga tggctagctc cgtcaacaac cgttgctcat cgactttcgc tccaagaaat
    37321 tcctccagct ttttttgata ccttatttga aattcctttt catagatttc tacaaaacta
    37381 tcgagttttt ttagtacttc tttgaattct tcagtattct ttttcaacac taaagctaaa
    37441 gcttgacctt ctttttctcg actcgcctca atagaatcaa gtgcgctttt caatgtatct
    37501 agagctaact gtgccaattc ttccatgctc tcttccggtt tttcttctac cgttacgaag
    37561 ttttcgtcca gcacgaagcg ttccgccatc cgactaattg acacttgatt ttgactaccg
    37621 tatcgttgtt gaattccgtc tgtcaattga gtgataagtg aatcaatcaa ttcccagttg
    37681 atgacgatct gtttttcgtt tttgccaata taagctagat tcacaaatac ttcgattcga
    37741 ccgcggctta actgctgttt aataagctga cgaaaatcgt tttcaacaaa gttcaggatt
    37801 ttaggacttc gtatttgaac atctagaaaa cgctggttga cactttttat ttcaatatct
    37861 aactgatacg cttttgtttc gcggatggct ttgccaaaac cagtcatact tttcattcga
    37921 cagggtcctt cctcattaca tattttctat tgctttttgc agttgttcaa actcttcgtt
    37981 cgtcaatgtc aggcctttcc ccattttttc atggtctggc gcccaatctc gaagatcgaa
    38041 ttttggcggg cgaccattcc agctgactag gttcagttct ttacgccacc ccttgttgtt
    38101 ttctgacaat atagcgattt cttctacgat ttcatatgaa aattcttgtg ccatggttgt
    38161 tcctccttga gataatataa atataggatc agtaatgtgt caactggttg cttaagcaat
    38221 tcatgctgca aacgatcctt gcgttttcct tgtagcagat aatcaaaata aacagttttc
    38281 gtgcgctcat tttgtatttt ttgctggtat tttcgatacc ttcgccattc ttcagtactt
    38341 aattgaccaa tttgtgccaa taaatcatcc ggcatacttt ttatatcttg ttggcagatc
    38401 atttgaatca tcatgtctac ttgatagatt tcattgggca gtaaaaaatg tttttttgct
    38461 aaaagttcta gcatcgccaa tccgcatgtc tctggtacga ccctgttctt atataaatta
    38521 cgcagttttt gccaaaattt tatgcttttg aaaaaagata attctaactg aatttctttt
    38581 gttaagtaag acaaaaatcc tcgccagtat gttcttgcag gtttaacaga atgaaatgta
    38641 taccagttcc catagtaata gcctaatcgt tttttcattt ccagatagcc taattgtaaa
    38701 tttaattggt tcctttggct atcaaaaagc agaaagctgc ctagtgtcca caatgtttga
    38761 catttccaat ggataaaaat gtctggcata cgaatttttt tcgctgggcc tggcccttgc
    38821 acatctacga caaatgcctc tgttgctcct ttgttaatcg caatatcaat aggtattttg
    38881 ttgcgatacc caccgtctgc atatttattt tttccgatct ttcgataagc catagcggga
    38941 taaaatgaag cagaggcgag tatccaatct ggtatttctt ctgtttttgt ctgttggata
    39001 tcagtcacta cttccgctag ttctggcagt ttagtagaca ccgtatataa acgtattttg
    39061 ggattatgtt tgagtaagga caaatccaaa ttgtctttga ccatcttttc caatggagca
    39121 gaagaaaccc cgccttcaat tagtgctgtt ttcgtcattt ggcgtctttc tcgatagaag
    39181 cgttcacgaa ggtcttcaac tgcagccatt tctggtaacg caagcacttg tcttgtcgac
    39241 agctttttcc ataaattcac agcttttttc atatctcctt gtaaaatcaa tgcgccgttc
    39301 aacgcaccta ccgaggtacc tgtgataatc tcaaagttga tatcatgttc cttcagcgcc
    39361 tgccacacac caatctgata agcaccgtgc gctcctcctc cacctaaaac aagtgctgta
    39421 tgatagacca gctttttatt taattgtgtg gttccttgat agccgttttt atgaaaaata
    39481 cgcaaccatt cttctgatag aggagattga aataaaaaac aacagttttc tttaataaaa
    39541 aaacgagcgc aagcttccat ataccgaaaa aattttttcc attcaaaagt ttcaccttca
    39601 atcaacagat tattaaaaat aacatctcct ctacttttag caatagtaaa atagacggta
    39661 tattttttcg atactgcttt aaaagcacat tgcttttttg cagaacgcca aaaagcgtct
    39721 ctactttctt gatgactttg aaaaaatgga aggggttgtt ttgcttgctg gataggcgaa
    39781 tacagcgaca ttttctgtga aaagtaaact ttttttatct ccatcgcgct ctcctttttc
    39841 ttcaagtata ccagtacgtc ataaaaaggt catgagtctc gtattcgact ttgtagattc
    39901 cataagaaaa caattcataa aacctccttt aaataaaaaa atacaaaaat cacaaaaatt
    39961 aattgaaaat gagagtgttt tcaatgaaaa cgtgattttg taatggaatt ttaaaataaa
    40021 agaaacaagc ttttttaatg caaatgttgt aaaataaaag agattaagag gaaataagag
    40081 gaggaacatg atgactgagt cagtttatgt acatattgat accacaagta atgctgtttt
    40141 aacaaaaggg ctgactgcta ctgactttgc aaacagtatc gtgcattttc ctcaaaactt
    40201 gctcttgctt gatccatctg cttctgctgg agaatatgaa tcccatacag ggctgaaagt
    40261 aatccgtggt acagaaaata ttcagcgttt cttttcctct tctcgtaaca ggcgtggatt
    40321 cgatgaattg aagtggatcg actttactga tttaacgatg ctgaaagaat tgacaccttt
    40381 agaaatttca gaattattat attttggtca tatgaaaaca catcttcatt cgccgttttt
    40441 ttataaacta cagaataatt ttgtatactt cgatcttaat gatcagctga atcggattta
    40501 ttatcgctat ttagatgaat tttataggat ttttgctagt aaattaagtt cgatcttgtc
    40561 agataagatc aatgtaaaaa aatctttttt caaaaaagaa acacctgttg aaaaaatcag
    40621 tgtggaatta ttgaaagaaa tgcgtaattt gatgcaggaa gggatcattt tttcttttga
    40681 tcaagttggt ttgatcgatg gtaaatatcg tatcccaatc tatgtgattg aagattatat
    40741 ttggaaatta aaaggcaccc ggtatcgaga agaggaaatc atcgctactt taatctactc
    40801 tgtcaccgat cgacactggc aattggtaga agatgccgat ctttcttatc tgccattgaa
    40861 gtagatctat ttcttatcaa aaaataaaaa ggaaacaaga aactaggaca tgattctgtc
    40921 tcaagtttct tgcttctttt ttatttacag ctcctgtcgt ttatctggca caaaaatcga
    40981 aggagtcgtt tgttggatcc ctaataaaac gatgatcgtt acaatagctg tactcaaccc
    41041 gctggcacca ttcattacga aagaaaagag ccaaggattc atcccccata gtgcatacgc
    41101 tccccagaaa accactcctg caataaaatg ccaaaaatat ctggaaagtg cgccaatcaa
    41161 tgcaccaaac acgatgcttt ttctaatttg tttcgtatta tcgtctacat agcctttttg
    41221 gacatttgaa gccatgagcc ctgctgcgcc tgcaaaagta taggcaatcg ggtactcaat
    41281 caacacttga acgacactta aataataaac ttgtcccaac ggaaaatgta ggatgcccca
    41341 aatgaaacct gccagcatcc ctgcctttgt ccctcttctt aacgcaaaaa gagtcaatgg
    41401 aatctgccct aatgaaatag aaaaactgct gccgatctgg atcggaatca acgacaacac
    41461 catcgctaac gctgcaacaa tcgttccttc tacccaaact ctattttttc ccataaaaaa
    41521 acttcctcct ttgaataaaa aagtcacaaa ggaggaagca gctttcgaaa atagtcaaac
    41581 agctgtttat tcacaattcc tacgctcgca taaccgatac aggtacgaag ggtttagaaa
    41641 tagattcaat ctcagcccaa ttggactccc ctttgtgagt ttatttatta tttatttttt
    41701 gtttcaaaca atggactaac tggtttgttt tcatggatac gtttgattgc atctcccatt
    41761 agtccgccaa cacttacttc atcgattttg tcgattttac gatcttctgg aagatagatc
    41821 gagtctgtaa caactagacg ctcgatagct gaatcttcta ttctttgtaa agcagggccg
    41881 gataatactg gatgtgtaca tgaagcatac acactagttg caccagcttc tttcaaagca
    41941 ttggcagcca atgtgatcgt acctgctgta tcgatcatat catcgatcag tacacaagtt
    42001 ttgcctttga cttgtccaat gatattcatt acttctgcaa catttgcttt tggacggcgt
    42061 ttgtctatga tcgcaatcgg agatttcaaa aattccgcta atttacgtgc ccgtgtgaca
    42121 ccaccatggt ctggtgaaac aacgacaaca tcatcgcctt tgattccttt ttctaagaaa
    42181 taattggcaa taagtggcgc acccatcaaa tgatcgactg gaatatcaaa gaaaccttgg
    42241 atttggactg catgaaggtc caaagtcagc attcttgttg caccagcttt ttgaatcatg
    42301 tttgctacta atttcgctgt gattggttcc cgtgcacgtg ctttacggtc ttgacgcgca
    42361 tagccataat aaggcatgac tacgttgatt gtttttgcac ttgctcgttt cagtgcgtca
    42421 atcatgatca gcaactccat caagttatca ttgactgggc tgctcgttga ttgaataaca
    42481 tagacatgcg atccacgaat actttcttca atgtttactt gaatttctcc atcactaaat
    42541 tgagtaacag atgattttcc taactcgacg ccgactgctg ctgcaatttt ctctgctaat
    42601 gggcgattgg aatttaacgc aaaaattttt aatcttgggt caaagtaatg ttttgacatg
    42661 gggacctcca ctttcttttt cacaactcta gaattctacc taataaatag tagtcatatt
    42721 ttttaaataa atcaagtgat tttgcaaaag tcttagttta gataagggag tttttttgca
    42781 tatccttctt tattgacttg tcttgctctc gcaattgcca gcgcatattc tggaatattg
    42841 tctgtaattg ttgacccggc agcaatcgaa ctgtttttag ctacctcgac aggtccgatg
    42901 atattcgtgc tagaaccaat aaagctgtgg tcgccgacag tcgtatgatg tttgttcttt
    42961 ccatcatagt tgacgaagac aacaccacat ccgacattga tttcttctcc cagcgtagca
    43021 tcaccaacat aagtcaagtg accgacttta gttcctttgc cgatttgagc atttttcact
    43081 tccacaaagt ttccgatatg gacgttttcg ccaatttcag cttttggtcg taaatgtgca
    43141 taaggtccca catctgcatg tttcttgaca tggctgcttt caatgactga gttttcaatg
    43201 acgacgtgat cctcaatcct actgtctacg atttttgaat gtgacccgat gacacaatca
    43261 gaaccaatga ctgtatttcc ttggatttgt acacctgctt cgatcaatgt gtctggaccg
    43321 atttctacgc ctgcatcgat gtatgtcgtt gctgaatcga caaagcttac gccattgacc
    43381 atatgcatct ggttaattcg ctttctcatg atctcatttg ctttagctaa cgcgatccga
    43441 tcattgactc ccattgattc ttcaaaatca tccgtttgat aagcagcaac tgtatggcct
    43501 tcttctttta agatttcgat gatatctgtt agatagtatt caccttgagc attgtttgtt
    43561 ccaactttgc ttaacgcatc gaacaaagct tcgttgtcaa aacagtaagt tcctgtattg
    43621 atttcttgta caagagcttc ttctggtgtt gcatcttttt gttccacgat tttttctacg
    43681 atcccaatat ggtcacggat gattcgtcca tatcctgttg ggttttctgc ttgtgccgtc
    43741 aagatcgttg cacttgcatt ttttccttgg tgatattcaa aaagattgtt cagcgtttca
    43801 gtagtcaata atggtgtatc gccactgata actaacgttg ttcctttttt tccttccaag
    43861 aaagaagcag cttgcaaaac agcatgtcct gttcccaatt gttctgcttg taacgcatat
    43921 ttactgcgtt ctcctagttg tgctttaact tgttctgctc cgtgtccaac gatcgtaatc
    43981 acttcatctg gtttcgtttc actgacccga ttaatgatat gttctaccat aggtttacca
    44041 gaaacgggat gtaaaacttt atataatttt gacttcatgc gggtaccttt ccctgccgcc
    44101 aaaataattg cataacgtgc gtccaactgc gtcactccta aatatagttt gctgatttca
    44161 gtttatccca gtatacccat tttttcaaca aaacaagtga ttttctttta tcccagcgtt
    44221 ttctggataa aaaaatagat agaccataac gggcctatct atttggtaac cggtgctctc
    44281 ctgccaactc ctcggtcaca agtcacacta ctcataaaac atgaaaagct gttttttgaa
    44341 atcgtttctt gtgacttaga gttaaacggt aggcttacaa cctcttcatt aaacgttttt
    44401 caatcagctg accttcggta ttagctcaat aaacggcaaa atatgaggag caatttttcc
    44461 gtttattgag ctaatactca ggacaggacg ctgatttcac aacctctaga taaacggtgt
    44521 tcaaaagcca gtttctcggt attaagccaa atttatgaaa aaatgtggaa agccattttc
    44581 acaaaatttt tcttactact cgaaactaag cggctttttc acaacctctt attctgaaaa
    44641 ataatttcct ggcacaaccg taatattttt cgtttgagta tctacatctt tgacataaag
    44701 cagtgacgta taatcatcga tggcgcgatg accgttgaat tttgattcgg caaatacagt
    44761 gatgccaaca agttctgatt cgaattcttc aatcaagctc ttcattccgt tgactgttcc
    44821 gccgcctttc atgaaatcat ccactacaag gacacgtgaa ccacgtttca aacttctctt
    44881 agacagttcc atcttttcaa ttctttcaga agatccagac acatagttga cacttactgt
    44941 tgatccttct gttatttttg agtcccggcg tacaataacg aatggcgcat tcagataata
    45001 ggacacagct tgggcaatag gtacgccttt tgtggctaca gtcattacag catcgatttt
    45061 ttcaccaagg tatttagaag caatgattct tccaacttga cgcagcagtt caggttctcc
    45121 taacaaatca gagagataaa catatcctcc tggaagcaga cggtcttgtt ctgatagacg
    45181 ttctgctaga gattcaatat attttttcgc ctcttcatat gaaatttcag ggatgaacaa
    45241 tacaccacct gcagcacccg ggattgtttc caaggttcca taccctcgtt ctttaaatgt
    45301 tttcttgatg attcccaaat cttcactgat agaagattta gcagattcat aacgttcggc
    45361 aaagcttgtt aaggaaatca attcatgcgg gtgattcaat aaatactgtg tcatgtcaac
    45421 tagacgttca ctgcgacgta ctttcaccta ttccaactcc ttttaagaaa ttttatcgtt
    45481 ttacattata taagtttttc ttcaaaaatt cgagcatttt atgaaaaa
//
LOCUS       DARBET010000016        44939 bp    DNA     linear   BCT 07-NOV-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4027
            SAMEA114569841-rid20112183.denovo.016, whole genome shotgun
            sequence.
ACCESSION   DARBET010000016 DARBET010000000
VERSION     DARBET010000016.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA114569841
            Sequence Read Archive: ERR12203666
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 44939)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 44939)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001972618.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001972618.1
            Genome Coverage       :: 132x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/07/2023 13:18:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,041
            CDSs (total)                      :: 2,975
            Genes (coding)                    :: 2,908
            CDSs (with protein)               :: 2,908
            Genes (RNA)                       :: 66
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 67
            CDSs (without protein)            :: 67
            Pseudo Genes (ambiguous residues) :: 0 of 67
            Pseudo Genes (frameshifted)       :: 26 of 67
            Pseudo Genes (incomplete)         :: 28 of 67
            Pseudo Genes (internal stop)      :: 20 of 67
            Pseudo Genes (multiple problems)  :: 6 of 67
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..44939
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA114569841-rid20112183.denovo.016"
                     /isolate="VRE4027"
                     /db_xref="taxon:1352"
                     /country="Denmark"
     gene            complement(38..1189)
                     /locus_tag="SCJ86_000969"
     CDS             complement(38..1189)
                     /locus_tag="SCJ86_000969"
                     /EC_number="1.1.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008267095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannitol-1-phosphate 5-dehydrogenase"
                     /protein_id="HEG1168550.1"
                     /translation="MRAVHFGAGNIGRGFIGEVLAANGFEITFVDVNHTIIDALNERK
                     EYEIELADESKEKITVKNVKGINNQTNPEKVIQEIAEADLVTTAIGPNILPFIAELIA
                     KGIQEREKEGSTTSLDIIACENMIGGSAFLEKEVYKYLPETSFTDKYIGFPNAAVDRI
                     VPLQHHEDPLFVQVEPFKEWVIDDSQRKNKKIQLKGVLYVDDLEPYIERKLFSVNTGH
                     ATVAYTGALLGYQTIDEAMQDALVVAQLKSVLQETGSLLIAKWGFDAEQHHAYIEKII
                     HRFQNKNISDAITRVARTPLRKLGYQERFTRPVRELQEHNLTCPHLTATMGIIFNYYD
                     PEDEQSRQLHEMKIHENLEQLIQEVTGINDPKTIGNIKQNVNRYAKQVA"
     gene            complement(1202..1639)
                     /locus_tag="SCJ86_000970"
     CDS             complement(1202..1639)
                     /locus_tag="SCJ86_000970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314097.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HEG1168551.1"
                     /translation="MQSLPFDMIELNRSFKTKEEAIRYCGQMLVEAGCVDEEYIDAMV
                     DRDKMLSVYMGNFIAIPHGTDEAKKYVKKSGICVVQVPDGVDFGTEEEEKIATVLFGI
                     AGVGDEHLQLVQQIALYCSDMDNVVQLADALSKEEVTENLAIA"
     gene            complement(1654..3420)
                     /locus_tag="SCJ86_000971"
     CDS             complement(1654..3420)
                     /locus_tag="SCJ86_000971"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS mannitol transporter subunit IICBA"
                     /protein_id="HEG1168552.1"
                     /translation="MERTIPKNNISLKARVQKLGSTLSSMVMPNIGALIAWGVLTALF
                     IPDGYLPNEALATMVSPMLTYLIPLLIGYTGGKVIAGDRGSVVGAIATMGVIVGTDIP
                     MMLGAMIMGPLGGYAIKKFDQLFQKRIKSGFEMLVNNFSAGLIGFGLALLGFSAIGPV
                     VDALTQAMAKGVEIILSAHLIPLTSIFIEPAKILFLNNAINHGILTPLGTEQVAEAGK
                     SILFLLEANPGPGLGVLLAFTLFGKGAAKSSAPGAMIIHFLGGIHEIYFPYVMMKPLL
                     FLSVIGGGMSGSFIFQLMDAGLRAPASPGSIIAILAMTPFNSYLPVILGVIVAAAVSF
                     GISAVILKADAKDTNEDFEKSVQETKAAKQASKGAQGIPQGATFSGVQKIIFACDAGM
                     GSSAMGASILRKKVQEADLPQTVTNQAISQLTDDSNTLIVTQVELQERAKQKAPSARF
                     VSVENFLNSPRYDEIIQELSSKQKTSVSEIENEPAEEFLSANIKQIKEILLVHDDRSG
                     SATMGMTVLKDILDKNHLTIPLRKVNIQDLKDEPDSLIVAKESLAEKVQMAAPQSIHL
                     SVNSMVNPQKYESIAQYLKQTA"
     gene            3684..4361
                     /locus_tag="SCJ86_000972"
     CDS             3684..4361
                     /locus_tag="SCJ86_000972"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335204.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEG1168553.1"
                     /translation="MTLLIKNMNMKRGKKRVIRQLNLLVESGEVAALIAPNGFGKTTL
                     LNGIAQLLPTTYDQLSLHNISPTAYTDYRRSFFFLESTNQLSDYLSAVDYLTLIKYYW
                     QSKVECQTVFQRLKMTEYLTLPIGKMSLGMKQHVLLAAYLISDSSVMLFDEPLNGLDP
                     GSIDILNSLIRQWQKEGKTILLSSHAIANIQSICSKACFLKNGEIVTESSNMEEILAI
                     YRKLYEV"
     gene            4364..5491
                     /locus_tag="SCJ86_000973"
     CDS             4364..5491
                     /locus_tag="SCJ86_000973"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287252.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEG1168554.1"
                     /translation="MSLLTFERNLLLKNKVNFLFPLLLVVLFAFPLFFDHKLAYTEFD
                     ELNHNYEEMQRLIETLKEDENEKEFVESLEKSNKLIEAILHAKNTGNVQQMVEATYHY
                     EKDILDRLISGQRQGIPIIEQQKRVELLRYMKEHQIQRYSIFDLPAHLPLANYYENIF
                     SGMISSFLILCITALFLSSIISYEKRKQVISLVNLLPDSMVKKHSIRFTIYYGAAMLS
                     LVMPFLIVSILVIIKNGLGDFRYPVGTIIGQEIRILPMYEYLFQSFLFLLLWVLFLST
                     ISFLLSALFEHSLVNLLGTLLCLFLAEYRLFSSIGWIESISHYLPTSYVDFQNVIIGG
                     DIFSPLASEQVTFMNGILTLGIWSIVLLFIGMGTIYIKKSY"
     gene            complement(5691..6131)
                     /locus_tag="SCJ86_000974"
     CDS             complement(5691..6131)
                     /locus_tag="SCJ86_000974"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346588.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HEG1168555.1"
                     /translation="MIQLPAEKILLDQSYQNWKEALTEIGQWMTQQGGIKPAYIQSMI
                     ERQEKASVYIGNFVALPHGTDKDEEVVEEGIYLVQVPDGVDFGTKEEPKIATLLFVVA
                     LKKEHQLAVLQDLAFFCSDIDQVMALSDAKTIEEVQMIFEHGHI"
     gene            complement(6142..8175)
                     /locus_tag="SCJ86_000975"
     CDS             complement(6142..8175)
                     /locus_tag="SCJ86_000975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374766.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BglG family transcription antiterminator"
                     /protein_id="HEG1168556.1"
                     /translation="MYLSPREKQLLAEFLSSPSPVSIQKMMNLLKVSKRTIYRELDNL
                     EVSLKSVNASLKKAARGSFVIEASEEVLEQLKENTDGKSNDLSTAKRQHAILAELLLT
                     TEPLSLAHFMESYRISNTTFYADIKQLEESIRQLPLEIIRNQGYEIEGPEKYRRLLTA
                     NVLELEINEYELFHSISFDASLNYFFRFVDPQHLSLARKVIGEELIQKKTNLSDRKLE
                     HLVLMLTLTMERVTKNHLLTNETYNSLANKELLQTAKRLFSRIASETKQLYPVNEIVF
                     FAGLLNDFTNSFDQNFFEETFDTQLAYLVKQLIQEVSEETNVHFYEDETLYKMLLTHL
                     SGVFSRAVLQEETLTNPILEKIMVQYEEVANAIRKAVERIFQEKKLSEEEIAYMVLHF
                     ANSLERSPKESNISIAGFSPSGLASTSMLELRLKRYFPFIQQIHFYRIADLKKIDLQE
                     EYDVVISTSILPGYGGKYILVSPLLLEDEVKQLKEAFSHIEKKKRQKITYNSNNEWMI
                     EDTYEETMHFMDQINQLLGRFFIQELDNPETISETIRSLFPYFPEGLVTDEEVVHRRL
                     LKRYEQAPIGIPHTNMGLFHTSSSLISKPIFCIFNLKHPLTIEGMDRQPVDLSRMLVM
                     LAPSPIQEKDGILLGKISGAIIMNDLNTEIFRSGNKEIVYQLLAKILIEEIKK"
     gene            complement(8177..9880)
                     /locus_tag="SCJ86_000976"
     CDS             complement(8177..9880)
                     /locus_tag="SCJ86_000976"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HEG1168557.1"
                     /translation="MEYKWKANNSSRVSMKQLRKAGHHLNEMIMPNLSLFIAWGILSL
                     ATKGVSGDLHTTLEEVCHWMIQLLLPILISYSGGKSVGGQKGAVAGAIASLGLIARSN
                     APQIVGAMIIGPLAGIVYEQFVSRFHVKFKAGYEMLVSNLLVGLTGSVICIIGIVFIE
                     PVLNSVHLLLASVVSWLVTMNLLPLVSLILEPLKVLFFNNAINHGVLTPLGIEFSQTV
                     GYSSLFLMEANPGPGLGILLSILCFAEKQEKVNASGALMIHAIGGIHEIYFPFVLLRP
                     YLFLAVMAGGASGTLIFQWLDTGLAAPVSPGSLLVIFANAPYPQLAGVSIGIAISTFV
                     TFICAAILLKLPNKEKENKTVKERGEILEPSIIHSVIFACDSGMGSSAMGAALLKKKL
                     AAEKIDLSVGYSSIYELNNQVNQLVIVQHELAGLAREKVPQSQIYVLDHFLETDILLS
                     RIKEAIQFPKREEKKEIADITEQKKSPANIVFLYKNNRRGSQTMGMTILSQLAKKAKK
                     QFVISKESIEKIKIQEETIYIAQRELAVTEQLEEKIPRLVLVEHWVATNEYEKLVKGE
                     I"
     gene            complement(9974..10924)
                     /gene="manA"
                     /locus_tag="SCJ86_000977"
     CDS             complement(9974..10924)
                     /gene="manA"
                     /locus_tag="SCJ86_000977"
                     /EC_number="5.3.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005879302.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannose-6-phosphate isomerase, class I"
                     /protein_id="HEG1168558.1"
                     /translation="MQEPMFLKPVFQEKIWGGSRLRSVFGFDIPNDKIGEDWAISAHP
                     HGVSVVENGEFKGKRLDELWSEHKELFGHPSEPVFPLLIKILDAEDDLSVQVHPDDAY
                     GMKHEGELGKTECWYIIDAEPGAEIIYGHHAKTREELAEMIKDGRWDDLLKKVPVKKG
                     DFFYVPSGTIHAIGKGIMILETQQSSDTTYRVYDYDRKDAQGQTRELHIQQSIDVTTV
                     PAKTPELQIKEVRKGNSSIVTYLETEFFNVYEWDIKGITSFKKQAPYTLMTVIEGAGD
                     LVVDGKTYPLEKGTSCIIPNGVSEWAVQGELAIIATEPGK"
     gene            complement(10963..11988)
                     /locus_tag="SCJ86_000978"
     CDS             complement(10963..11988)
                     /locus_tag="SCJ86_000978"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324200.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-lactamase family protein"
                     /protein_id="HEG1168559.1"
                     /translation="MYPHTKQLILKKMEEGFYPGVAVAFIKENQVETFTAGFAQVVPE
                     KQLMTEDLLFDAASLTKVVATTSLVLRLLEDGKIELDQPLHKYLPEFENQTITLRHLL
                     THTSDIKTWIPNRDKLSQEELIAAYLKLEPGEQLGKEVKYTDAGTILLGLMLEEIYQE
                     TAVEIFQEQVLEPLGMMNSLFLPFPSKKIVPTEQLPSGEVLRGQTHDPKARVLAEHAG
                     NAGLFIDLKDSITFVQMYLNKGKIRNGRFLEEETIDSLLQDQTPKKNGYRSLGWDLKK
                     DVEDGHAILFHTGYTGTFFLIDIKTQEALIFLSNRVHPVDHRAEYLICRDEIVENFLK
                     EKAVLPK"
     gene            complement(12197..13066)
                     /locus_tag="SCJ86_000979"
     CDS             complement(12197..13066)
                     /locus_tag="SCJ86_000979"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extradiol dioxygenase"
                     /protein_id="HEG1168560.1"
                     /translation="MKDFQLGAATYLKSVAIRVKDRDKMIDFYKNVVGFDLKREENEL
                     AILGTEEAGSEMLLLEESPRANDHMGVIKKLNRFSLIIPSTEEMADILCRIRKMNYPI
                     KEALEDHGRMGILLVDPEENELEIYHEENKNDQREIPETMDQEALLRKSEEKFSKLST
                     GSYFEKIHLNVTNLTKERQFLNHVLGLKVHNEEEGIHVLNEGDFHVGLTEASGGAIDL
                     PTDEVIGLDFLKFMIDTESMEGLIAHLQQLSVDFFLDKKHTVLTIYDAIGIEWWFVSE
                     PKEKNKTDIRCDA"
     gene            complement(13525..15417)
                     /locus_tag="SCJ86_000980"
     CDS             complement(13525..15417)
                     /locus_tag="SCJ86_000980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853211.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEG1168561.1"
                     /translation="MSKTNTRPSHGPAGGPGHGGPGRNLGVKGEKPKNFWGTVRRLFG
                     YMSKRMVAIIAVFVLAIAAVIFQIQTPKVLGKATTEIFKGVMAGAQQMQQGQQVTKFP
                     IDFDKIAQIIATVIVMYLISAVFNFLQQFIMTRVSQRTVYELRHDLEAKMNRVPISYY
                     DTHTNGDIMSRAINDMDNIASTLQQNLTQFVTSAVTLIGVIVMMLSISWQLTLVALLM
                     IPLSLIIVMIVAPKSQVFFADQQKSLGLLNNQVEETYGGHTIVKTFNHTEKDQEVFEK
                     ENQNLYAAGWKAQFISAIIMPLMNFVKNLGYVFVAVLGGVKVANGNMTLGDVQAFLQY
                     TTQFSQPITQLASLMNTIQSTVASAERVFEVLDEEEMSNEKSGLPEEKDTPYKVRFEN
                     VQFGYTPDNLLMTDFNLDVKPGEMVAIVGPTGAGKTTLINLLERFYDVSGGSIRYDGV
                     DTRDLSRDELRAQFSMVLQDTWLFTGSIYDNIKYGNDDATDEQIIEAAKAAHVDDFVR
                     KLPEGYNTVLNEDASNISQGQRQLITIARAFLANPDVLILDEATSSVDTRTEILIQKA
                     MNRLLENRTSFVVAHRLSTIRDADNIIVMNHGSIVETGNHDTLMEKDGFYADLYNSQF
                     SEEEAS"
     gene            complement(15414..17141)
                     /locus_tag="SCJ86_000981"
     CDS             complement(15414..17141)
                     /locus_tag="SCJ86_000981"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736658.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEG1168562.1"
                     /translation="MLKIVKRISLTSAIAAAVFMVIQVLADLYLPTLTSNIIDKGVAQ
                     GDVDYIWHTGFVMIGFSLISILAAIANTFFATRESQKLGKQLRTDVYKKSESLTKDAF
                     DKYGTASLITRTTNDVTQIQMVTQMFLRMMINAPITLIGASILAYQKDHQLTKIFLVV
                     IPVMIILIGGIMYFAVPLFKSMQKKTDRLNLVFREGLTGVRVIRAFDKTRFEENRFDL
                     ANKDYTNTAIKVNTIVALMMPMMTLIMSGTNVAITWFGGHYIADMTLEVGNLIAFMTY
                     AMQILISFMMLSAIFIMVPRAQASADRINEVLDEKIGIHDPENPKTVSFAGKNATLAF
                     NHVNYRYHGAEKLALEDIDFQAKSGEIVAIIGGTGSGKTTLVNLIPRLYDIESGSIQI
                     NGTDISDMTQYNLRELMGFVPQKAVLFSGTIRDNMQYGKPDATDEMIWKALEIAQAKD
                     FVSEMEDGLDSHVEQGGGNFSGGQRQRLAIARALVKTADIYVFDDSFSALDFKTDANL
                     RQALKTNMKESITVLVAQRVSTVMDADMILVLDEGKLVGKGTHEELLATNETYQEIVH
                     SQLREEDLA"
     gene            complement(17157..17807)
                     /locus_tag="SCJ86_000982"
     CDS             complement(17157..17807)
                     /locus_tag="SCJ86_000982"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629554.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR family transcriptional regulator"
                     /protein_id="HEG1168563.1"
                     /translation="MPTQTFFHLPKEKQKRLIEAARIEFSRVPLKEASIANIVKLADI
                     PRGSFYQYFEDKEDLYFYYFETMRRDSSKDMLQLMKESDGDLFKGFESYFSKMIYEIL
                     EGENASFYRNLFMNMDYRASRKVAPHTSRQKTPHDPHKEHKQDAKELLELIDRTKLKV
                     KNDHELELLIQLVMNTVFTTIAHSYRLFSTSEDYSVEQAISEFNLKLSWLKNGVYK"
     gene            18011..18307
                     /locus_tag="SCJ86_000983"
     CDS             18011..18307
                     /locus_tag="SCJ86_000983"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350925.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhodanese-like domain-containing protein"
                     /protein_id="HEG1168564.1"
                     /translation="MAQSISVQDFNKLPLDADSIVLDTRNTLDYTKNHLSHSLSIPAA
                     ILPRQLKNLDPKKLYYILSYTGRRSEALADQLTANGFRAVYIIGGMEALQSSVA"
     gene            18479..19099
                     /locus_tag="SCJ86_000984"
     CDS             18479..19099
                     /locus_tag="SCJ86_000984"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340253.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FMN-dependent NADH-azoreductase"
                     /protein_id="HEG1168565.1"
                     /translation="MASVLVVKGHPLTAEESRTVKALTSFLTSYKENHPDDEVTVLEL
                     YRDDIPEIDEELLSGWEALRAGAEFTSLSESQQQKIARFNELTDQFLAADKIVIANAL
                     WNLNIPTKLKAWFDTVNVAGKTFRYTENGPEGLVTGKKALHIQSNGGVYNGQDFASQY
                     VKGILNFVGIDQVDQLFIEGIDYDPDRADELMQNALDKAAALGKSF"
     gene            complement(19191..20357)
                     /locus_tag="SCJ86_000985"
     CDS             complement(19191..20357)
                     /locus_tag="SCJ86_000985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291475.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter"
                     /protein_id="HEG1168566.1"
                     /translation="MEGKNKKNRPVSWFWRWFLNNQVVTALLVVLLILLIVFLFTKVS
                     YLFAPIWQFLAIVGLPIILAGILYYLMNPVVDYFEKRKVPRLYSIIGLFVLVVALIAW
                     GSVVIIPKIQEQTVSFISNFPQYVDTIDNKLTEILRDPLFNQFREQLETAGDKFMNSA
                     GDMIQDISKSTVQSLGSFVGAVATILVALLTMPFILFYLLKDGKQLAPYFVKFLPTRM
                     QKPTLNVLSEMNSQVSSYIRGQLTVAFAVAIMFMIGFAVIGLEYAVTLGIIAGFLNLI
                     PYLGSFLAMIPAIFLAIVAGPFMIIKVLIVFAVEQTIEGRLISPLVLGNQLAIHPVTI
                     LLVLLTSGKLFGIIGVILGIPVYAAAKVVITHVFEWYTTISSLYEENPLKKQKE"
     gene            complement(20369..20722)
                     /locus_tag="SCJ86_000986"
     CDS             complement(20369..20722)
                     /locus_tag="SCJ86_000986"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328164.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucitol/sorbitol transporter subunit IIA"
                     /protein_id="HEG1168567.1"
                     /translation="MIQAKITEIGTEAINKEEPMLILFDKTATAALRKYSVIQEVSKD
                     EPFSLKAGGTIAFDDQVYTIDYVGPMANGNLETVAHVSLIFDACPEEGQIANGIYLHP
                     HRLPAIGLGSQIFYN"
     gene            20850..21872
                     /locus_tag="SCJ86_000987"
     CDS             20850..21872
                     /locus_tag="SCJ86_000987"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310080.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lactonase family protein"
                     /protein_id="HEG1168568.1"
                     /translation="MIEKIILGTYTRRESKGIYTIDLDTEKEELANLSLAVEENSPTY
                     VAKSKAGNLYTVTSVDGLGGAGAYDKDFHFLNAVTESGAPLCYVAVDEARQLLYGANY
                     HKGEVNVYHILGDGELKAADSIFHQEATGPHKNQDHAHVHYTDLTPDQRLVVCDLGTD
                     RVYTYDVSENGKLNLVTTFTAEPGTGPRHLVFHPNGQFAYLFGELDSTVRVLSYNKET
                     GAFEELQKISTLPEEFDGENGGAAIRISNDGKFLYASNRGHNSIAVFAVFEDGSHIQN
                     KQIISTEGDFPRDFALDPSNNFVVCANQNTDNLTLYRRHPETGLLELLKKDVYVPECV
                     CVYFDR"
     gene            21891..22052
                     /locus_tag="SCJ86_000988"
     CDS             21891..22052
                     /locus_tag="SCJ86_000988"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEG1168569.1"
                     /translation="MNQQDVLDRLREELNIPFFDGKIEDKEYSEEEYQKLKADLQNYF
                     EQYVQNVEN"
     gene            complement(22311..22745)
                     /locus_tag="SCJ86_000989"
     CDS             complement(22311..22745)
                     /locus_tag="SCJ86_000989"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEG1168570.1"
                     /translation="MLPNIGYYVQSIDQIVKETEAIGEGMHPKYEVIRNAIDEGKTSE
                     LDKSTLEEIILLFEEGTKKYRAMLKKVSTLRPPAKAMGIHKNFEKAYLSYVAGCEEMI
                     QSIDLESGVDEDLFNTSEAKQDQATDQLTAAIQKMTNLLLKK"
     gene            complement(22855..24714)
                     /locus_tag="SCJ86_000990"
     CDS             complement(22855..24714)
                     /locus_tag="SCJ86_000990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374787.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LTA synthase family protein"
                     /protein_id="HEG1168571.1"
                     /translation="MKRYISRLSTKQKKILKGTAAIISIFFLVVFSNLFLQWCQNDLS
                     VDLALKFAFSWHTEKFFLACLVLLIILIFLIALAGSVPLGSLTYVVAIGVLGFANYMK
                     MSYRQEPIYPDDLKMITEIGLLKDMTGTMLFTVILAAAGTVIGLFCWYMFRSLKKGRR
                     FQLIRLTTLLVAIGLLGYISNFNNPDNLLRKAYNKTALWIPYSQKMNYYNTGFIGGFL
                     YNLKVEPMDEPEGYSKEKIKEITEKYQKLADEKNKTVEEESPNIVFVMSESFSDPSRL
                     NGVEVSGEPLADYYEVADQTYSGNMLSQNYGGGTANIEFEALTGFSMALFNAQLTTPY
                     TMLVPKMDQLPSIVSALNAQSYQTTAIHPYNTSMYKREDVYQTLGFDQFISERTMTYT
                     DTIENNPYISDESAYKEVMTLLKEEKTPQFIHLVTMQTHMPYDGKYDNLDYPAKTSDG
                     SGTLDLENYLQDISYSSTALKQFTEELKNLSRRTLVVFWGDHLPGIYSDTIQAKNDKQ
                     TLHETQFLMFDSKGELEKQTTHDAITSPFYFAANLMEQTNQTTNGFYQLLLSLEQELP
                     AFERELYYQNGQWYKEAQFNRSQQEIYDEYQLIQYDIVAGKQYSLADGFFEHE"
     gene            complement(24832..25992)
                     /locus_tag="SCJ86_000991"
     CDS             complement(24832..25992)
                     /locus_tag="SCJ86_000991"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-3-phosphoglycerate dehydrogenase"
                     /protein_id="HEG1168572.1"
                     /translation="MYHVHLIDAIPEQEIEKLQGEYFKIIDDLPAEGIVIRSTKVKDE
                     WLTPDLLAISRAGVGVNTINVEKASENGTIVMNTPGVNANAVKELVLCCLLLSSRPII
                     EASRMVQTLTGPNILEQAENKRSAYVGRELQGKTIGLLGLGAIGTKVALSCYSLGMDV
                     LGYSIRDAQLDYVRQADLETVLSTSDYIVVMLPLTEDTKGLIDQANIEKMKKDAVLIN
                     VGRSEIVDKYAVMQALEKQHLAKYLTDFPEEEFLENDRILMLPHLGGSTQEALADSGR
                     LAVEALKDYLLFGTVREAVNYPSARMFFQAPFRFTIFYQKKTNILAEIFSLLNENELA
                     IADISRNHKNEHVYILIDIDSTDFEQLNQVKQQLEKISGVRKVRLLKKPERP"
     gene            complement(26299..27723)
                     /gene="araA"
                     /locus_tag="SCJ86_000992"
     CDS             complement(26299..27723)
                     /gene="araA"
                     /locus_tag="SCJ86_000992"
                     /EC_number="5.3.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287282.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-arabinose isomerase"
                     /protein_id="HEG1168573.1"
                     /translation="MLNIGEKEFWFVVGSQHLYGEEALREVKKHAQEMVDELNENGQL
                     PYPIRLQELAVTADTITKIMKEVNYREEVAGVITWMHTFSPAKMWIRGTKLLQKPLLH
                     LATQYNESIPWKTIDMDFMNLNQSAHGDREYGFINARLNKQNKIVVGYWKRPEIQKEI
                     ADWMDVAVAYNESFGIKVARFGDNMRNVGVTEGDKVEAQIQFGWTVDYFGIGDLVQVI
                     DRVSDEEVDQLFEEYKELYTFDYGDYEEKTWEEHVKVQAQQEIGIRRFLEEGGYNAFT
                     TNFEDLYGMKQLPGLAVQRLMAEGYGFAGEGDWKTAAIDRLLKIMARGKDTGFMEDYT
                     YELASGQEAILESHMMEVDPTLAATKPRIVVSPLSMGDREDPARLVFDGKAGEGVVVS
                     MADFGTHYKLLINEVEAFEPTTEAPNLPVARVLWKTKPNFHEGVHSWIQAGGGHHTVV
                     SLNLTTDQIETWAKLVELETVVIR"
     gene            complement(27739..28437)
                     /locus_tag="SCJ86_000993"
     CDS             complement(27739..28437)
                     /locus_tag="SCJ86_000993"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005873905.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-ribulose-5-phosphate 4-epimerase"
                     /protein_id="HEG1168574.1"
                     /translation="MLEQLKEEVYQANLDLPKHGLVKYTWGNVSAFDPETRYFVIKPS
                     GVSYEEMTADDMVVVDLDNHIIEGKLNPSSDTPTHAVLYRSFPQIGGIVHTHSTWATI
                     WAQAGLDVPAMGTTHADTFYGSVPCARFLTQQEIDSGYEYETGKVIIETFKERKIDPL
                     AIPGVLLHGHGPFTWGKDAQSAVMNAVVLDEVCKMNLFTRQLNSFSEELPQRILDKHY
                     LRKHGKNAYYGQNK"
     gene            complement(28394..30055)
                     /locus_tag="SCJ86_000994"
     CDS             complement(28394..30055)
                     /locus_tag="SCJ86_000994"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294116.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FGGY-family carbohydrate kinase"
                     /protein_id="HEG1168575.1"
                     /translation="MKEVNAQSMLDVIDGRTSLGIEFGSTRIKAVLIDSSFTPIAAGS
                     YEWENKLENGIWTYSLEDIWKGLRVSYRKMAAEVFEIYGENLTTIGSIGFSAMMHGYL
                     AFDSKEQLLVPFRTWRNTITEEAERILTEKFQYNIPQRWSIAHLYQSILNKEEHVKEI
                     NFLTTLAGYVHWQLTGEKVLGVGDASGMFPIDPSTKTYHQEMKNIFGQLIEKAHVAID
                     LDHILPKVLVAGEEAGKLSESGAYLIDPTGNLRPGIPVCPPEGDAGTGMVATNSVAKR
                     TGNISAGTSAFAMIVLEKELENVHPEIDMVTTPSGELVGMVHTNNCSSDINAWVSIFE
                     EFTNSLGMEISRDKLFTVLFNKALEGDTDCGGLLSYGYFSGENITGVNEGRPLFVRTP
                     GSRFDLANFMRIHLASAFGAMRIGMDILKSENVQIDRLVGHGGIFKTPEVGQRILASA
                     MEAPVTVMDTAGEGGAWGIALLAAYMMDRHGKSLENFLAEDVFGQDQGITITPSAEEI
                     AGYQIFMRRYREGIDIEKTAIHQLQEKGVIENAGTTERRSIPSKS"
     gene            30273..31673
                     /locus_tag="SCJ86_000995"
     CDS             30273..31673
                     /locus_tag="SCJ86_000995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar porter family MFS transporter"
                     /protein_id="HEG1168576.1"
                     /translation="MKKEKKVSSSFIYFFGAFGGILFGYDIGVMTGALPFLQHDWGLA
                     GKASLIGWITSSVMLGAILGGSLSGLLSDKLGRRKMILLSALIFMAGSVLSASAPHNG
                     SYFLIAARILLGLAVGAASALVPAYMSEMAPARLRGRLSGINQVMIASGMLLSYVADY
                     LLKVLPETMAWRVMLGLAAVPALILFFGVLALPESPRFLMQSGRLEEAKRVLNYIRTP
                     KEAEQEFEQIQLNVKQEKTTGTSWHTLFLEKYRSLVIAGIGVAVFQQFQGANAIFYYI
                     PLIVEKATGHAASDALMWPIIQGIILVAGSLLFLVIADKFNRRTLLKIGGSVMGLSFI
                     LPAVLGTVLDAHTNSLLILLFLCIYVAFYSCTWAPLTWVIIGEIFPLAVRGRASGLAS
                     SFNWIGSFLVGLLFPVMTASMSQEIVFGIFGIICFLGVLFIQEIVPETRGKSLEEIEQ
                     SASKKTYPKRISIHHS"
     gene            complement(31925..33004)
                     /locus_tag="SCJ86_000996"
     CDS             complement(31925..33004)
                     /locus_tag="SCJ86_000996"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291458.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HEG1168577.1"
                     /translation="MAKYQDIAEEIKQKILREEYEVNTTIPSEFKLQEIYDVSRHTIR
                     QAIAVLVNEGYLRKEKGSGTYVDDSYKKEKTPLNRTKTIGVITTYLSDYIFPSIIRGI
                     ENELRESGYSLLLASTNNDHELEKECLKKMIAYGVDGLIVEPTKSNQYNPNLALYVAL
                     REQGIPMVMINAAYEELNASYICLDDVKVGFMAAEYLIKKGHQRLLFITKIDDLQGKY
                     RMKGFISACEQYNVRFLTEDIITFTTETRETIAEKTVQRLKEEQALDGIICYNDQIAS
                     LMLDKLTENGYHIPDDLSIVGTDDSSLSHLGNKKLTTLTHPKEKLGQDAARWIVTTIE
                     KGQAQPNILYEPVLVERETVKENEK"
     gene            complement(33090..34049)
                     /locus_tag="SCJ86_000997"
     CDS             complement(33090..34049)
                     /locus_tag="SCJ86_000997"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738651.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 43 protein"
                     /protein_id="HEG1168578.1"
                     /translation="MMKTLYNNPIVRQRADPWVYKHTDGYYYFTGSVPGYQVIELRRA
                     KSLNELEHAASLIVWQAHEEGPMSELIWAPEVHYINGKWYIYFAASNHKTIRDESHHH
                     RMFVLENQHIDPMNTDWIEKGQIKTKRETFSLDGTTFSSQGKLYYVWAQQDPEITGNS
                     NLYLSEMDNPWTLKGKQILLSIPEYEWEKRGFSVNEGPAVLTRNGKIFITYSGSATDE
                     NYAMGLLWADEHKNLLLKESWHKLSEPVFVSSEKNKQFGPGHNSFTVSEDGCFDILIY
                     HARPEKNQKGDPLDNPNRHANAQCFTWNEQGFPEFGEPVPYAL"
     gene            complement(34061..35572)
                     /locus_tag="SCJ86_000998"
     CDS             complement(34061..35572)
                     /locus_tag="SCJ86_000998"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338659.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-N-arabinofuranosidase"
                     /protein_id="HEG1168579.1"
                     /translation="MKVKIREDFTVGEISSRLFGSFVEHMGSVVYTGIYEPGHETADG
                     DGFRQDVLSYVKELELGVIRYPGGNFTSGYDWVDTVGRKEQRPKKIDLAWRGIEPNSF
                     GLHEFFKWISHVQAKPIFTVNLGTKGIDEAKNLVEYCNFPKGTFWSDLRIKNGQKKPF
                     GVKLWCLGNELDGPWQVGAKSAYEYGRLANEASKVMKLVDDSIETILVGSSTPRLPSY
                     PEWDRIALEEAYENVDYLALHNYIDKYDEEDLTKPPKNERDDTATYLAKAYRFDRQIE
                     EIVATCDYVKGYLRSEKTMYLAFDEWNVHAQPEKRHRDFEVGSPIDWCYFTMEDTLLF
                     GSLGLAILRHADRVKISCQSLLVNTIPLILTDAKGQAWRNPTYYVMQHLSKYAKGQVL
                     KQQIICPVYDCEKIKNVPYVDSVIVDNGNQLTIFLINRTNEQQKITIEHDFNLLDQGE
                     HYTLTSSDLLDKNTREEPEKIKPEINYFSIEKHDELNVGIEKYSWNVIRIKKM"
     gene            complement(35594..36157)
                     /locus_tag="SCJ86_000999"
     CDS             complement(35594..36157)
                     /locus_tag="SCJ86_000999"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297690.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YesL family protein"
                     /protein_id="HEG1168580.1"
                     /translation="MKIVETKFYGQLIVVSDYILLGILWVIVCLPVITIVPATCAVFY
                     VMAQWKGEGSGNIIYFFFQGIKQHFGKNLFLSLLTIIIYFLVNQLLEEQTIVLSISGY
                     VVLLIYSMFLLSWIYVISENKDITFLQIFEQSAWKVFFDFLRNILCCVLLLGFVLLIF
                     LFPPFIFIFAGGVWKLVSIILTIGNER"
     gene            complement(36163..37026)
                     /locus_tag="SCJ86_001000"
     CDS             complement(36163..37026)
                     /locus_tag="SCJ86_001000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HEG1168581.1"
                     /translation="MAPESSITVTVNSRKAVSGKKIIRTISMLVILLVIAFPFLWLII
                     SSFKHEKDIISFPPRIFADSYTLDNYIKVWTTIPLLDYIKNTVIFAGGTVITSVFFDS
                     LAGYAFARMRFKGKSVLFYFVLLTMMIPFQVFMIPLFIEVNLLGMLDTYAGLIIPRMT
                     TAFGIFMMRSFFITLPDSLEEAARIDGLSEFNIFLKIMLPLSKPTLLSLGIFTLMNSW
                     NDLLYPLILTSSSKMRTLPAGLALFTGQNISFYGPVMAGTVISMLPLLVVYIFAQKYF
                     VQGTAMSGMKE"
     gene            complement(37040..37936)
                     /locus_tag="SCJ86_001001"
     CDS             complement(37040..37936)
                     /locus_tag="SCJ86_001001"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287294.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HEG1168582.1"
                     /translation="MNGFTKYWNRPDRAAYLFLTPSFLVLLLFTVIPLLGTFAISFTD
                     LNIFFTSRNFVSFENFTRIFSDERAVNSLIHSLYFTVLETPTQIIVGLLAAVALSKNT
                     RFNRFCRSTFYIPVICSLTSIAIIFSMLLDPNVGAIPYLFSQVGLPIPQFFRDPTLAM
                     PTVAIMTVWKNFGVTMTILLTAIQGISPSLYESAQMDGATNRQQFFNITLPQIVPSLG
                     FCILTNLIGSMQVFDQVYVATAGGPQFKTETAVQYIYSRGFTAPYELGYASALSSVLF
                     IIVATIAISTNVYMSRKEKQMK"
     gene            complement(38111..39400)
                     /locus_tag="SCJ86_001002"
     CDS             complement(38111..39400)
                     /locus_tag="SCJ86_001002"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HEG1168583.1"
                     /translation="MKEEQKMKGKKWLIGSLLVAATGILGACGGGSSAESDSDKEVTL
                     TFWNGFTASDGEILQEIVNEFNKTNDKNIKIEMDIMTWANLNEKLPTAISSKKAPDFI
                     ALNYPDFAQYVENGAVQPLDDFWEYDGVDKSDFKETAIELGEVEGKQYFVPMQVQGMY
                     MYWNNDLFKQAGLDTEQPPTTWEELTEMAPKLTDSSKNVSGFVFNKDGTAPLYNWMLA
                     NGGKLVNDDYTKSEFASPENLKTLKAIQKMIHVDKTGPESISGAEMDNLMNAGQLAIE
                     INGPWLNNGLKANEIDYNVTTLPQGSDSDKKTAILDGVGYAIPASTDNNKKEAVYEFL
                     KYWNTTEIGKKWSIENGFPAYLDSVAKDEEVKNDPIVSELSKQMDYAEPYLPGFTQIS
                     AINNDIINPLIEKLLAGEDPETLMNDADDAINQLLQK"
     gene            complement(39372..40889)
                     /locus_tag="SCJ86_001003"
     CDS             complement(39372..40889)
                     /locus_tag="SCJ86_001003"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-N-arabinofuranosidase"
                     /protein_id="HEG1168584.1"
                     /translation="MEATMLISSVKRVGKVDPRLYSSFIEHMGRAVYEGIYQPDHPSA
                     NKSGFRQDVIHLVKELNIPMIRYPGGNFVSGFRWEDSIGPIANRPRRLDLAWRTIETN
                     EVGIHEFYNWCQHVGAEINMAVNLGTRGVDAARNLVEYCNFPKGTYWSDLRRSNGQES
                     PFGIKTWCLGNEMDGPWQIGHKTAEEYGRLAEETAKAMKLVDDSIEVVLCGSSNRQMP
                     TFGDWELTVLDQAYDQIDYLSLHQYYGNQKNDLSHYLARSLDMDRFISEVTAMCDTVK
                     AKKHSKKQINLSFDEWNIWYHSAEQDEQAAPWQIAPPLLEDNYNFEDALLLGCLLITL
                     LKHADRVKIACLAQLVNVIAPIRTETNGEAWRQTIYYPFMQAAVYGQGEVLSPQIRSA
                     NYATEDFEEVPYLESIAVINDKELVIFAVNRGEEEMNLTVHLSELSLEGVIDFSEMSG
                     FDTKATNLPKSEQVKPSHSTNVKAEDGKINCLLKPLSWNVVRCRLSDEGGTKNER"
     gene            41087..41977
                     /locus_tag="SCJ86_001004"
     CDS             41087..41977
                     /locus_tag="SCJ86_001004"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736676.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ArsR family transcriptional regulator"
                     /protein_id="HEG1168585.1"
                     /translation="MQLQINEESLPVYEALASKTRIKIIQLLSKKKMNVKDLAKELGV
                     SSAITTMHVKKLEEANIIKTEKVGQQKISSLRVDKIDISFPEKIFNAFDTKETSIPIG
                     HYTNYAIEPTCGLATIHDFIGKVDEPRYFMDPRRMDARILWFTSGFVEYQAPNFLNTE
                     DTLEMLEVSVEISSEFPFSNDNWPSDITFSLNGVELGTWTSPGDFADIRGKYTPDWYP
                     DNLNQYGLLKTIRITKHLTNMNGEPLSNITINDIPKEQDTWHLRIEVKDDAKHVGGCT
                     LFGKGFGNYDQDIKLKVYYS"
     gene            complement(42050..42874)
                     /gene="nadE"
                     /locus_tag="SCJ86_001005"
     CDS             complement(42050..42874)
                     /gene="nadE"
                     /locus_tag="SCJ86_001005"
                     /EC_number="6.3.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ammonia-dependent NAD(+) synthetase"
                     /protein_id="HEG1168586.1"
                     /translation="MSLQTEIINELGVKPTIDPKEEIRKSIDFMKAYLKKYPFLKTFV
                     LGISGGQDSTLAGRLAQLTMEEMREETKDENYQFIAVRLPYGEQADEEDAKAALDFIQ
                     PDVSLRVNIKPAVDAQVQVLSEAGVGISDFNKGNIKARQRMITQYAVAGERAGAVLGT
                     DHAAENITGFFTKFGDGGADILPLFRLDKRQGKQLLQALNAPEKLYTKIPTADLEDGK
                     PMIADEVALGVTYNEIDDYLEGKEVPAQAQEKIEAWWNKTQHKRHLPISVLDDFWK"
     gene            complement(42886..44358)
                     /locus_tag="SCJ86_001006"
     CDS             complement(42886..44358)
                     /locus_tag="SCJ86_001006"
                     /EC_number="6.3.4.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287304.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinate phosphoribosyltransferase"
                     /protein_id="HEG1168587.1"
                     /translation="MNNTYPDDSLTLHTDLYQINMMQTYWELGRADLHAVFECYFREM
                     PFNHGYAVFAGLERLVNYLENLTFNDSDIAYLRGLEVYPEGFLEYLQNFEFKATVRSA
                     REGELVFANEPLIQVEGPLAHCQLVETALLNMVNFQTLIATKAARIKSVIGEDPLLEF
                     GTRRAQELDAAVWGTRAAYIGGADATSNVRAGKIFGIPASGTHAHSLVQSYGNDYEAF
                     MAYAKTHKDCVFLVDTYDTLKSGVPSAIRVAKELGDKINFQGVRIDSGDMAYISKRVR
                     EQLDAAGFTEAKIYASNDLDEATILNLKMQKAKIDVWGVGTKLITAYDQPALGAVFKL
                     VSIEDDEGKMMDTIKLSSNAEKVTTPGKKQVWRITRNFDGKSEGDYVTLWDEDPREEE
                     AIFMFHPVHTFINKTVRDFTARPVLQDIFIEGKRVYELPALNEIKEYTKENLDSLWEE
                     YKRDLNPQKYPVDLSTECWNHKMAIMERMKKSVAELHTEA"
BASE COUNT        12989 a         9387 c         7783 g        14780 t
ORIGIN      
        1 aaaaatttcg tcacattccc ctttttcgta tcctatttta agcgacttgc ttggcataac
       61 ggttcacgtt ttgcttgata ttcccgattg ttttcggatc attgattcct gtgacttctt
      121 gaatcaattg ttccaagttt tcatggatct tcatttcatg caattgacgg ctttgttcat
      181 cttcaggatc atagtaatta aaaataatcc ccattgttgc cgtcaaatgt gggcaagtca
      241 agttatgttc ttgtagttct ctcactggac gtgtaaatct ttcttgataa cctaacttac
      301 gtaatggtgt ccgagcgaca cgtgtgatcg catcagagat atttttgttt tggaagcgat
      361 gaatgatttt ttcaatatac gcatgatgct gctctgcatc aaacccccat ttggcaatca
      421 gcaaacttcc tgtttcctgc aaaactgatt ttaattgtgc cacaactaac gcatcttgca
      481 ttgcttcatc aatggtctgg taacctaaca aggcacctgt ataagcgaca gtcgcatgac
      541 cagtattgac actaaatagt ttgcgttcga tatatggttc tagatcatct acatataaaa
      601 ccccttttaa ttggattttt ttattttttc tttggctatc gtcaatgacc cattctttga
      661 aaggttccac ttggacgaat aaaggatctt catggtgttg taatgggacg attcgatcga
      721 ctgctgcatt tggaaagccg atatatttgt cagtaaatga tgtttccgga agatatttat
      781 acacttcttt ttctagaaat gctgaaccgc caatcatatt ttcacaggca atgatatcta
      841 aagatgttgt gcttccttcc ttttctcttt cttggatacc ttttgcaatc aactcagcaa
      901 taaaaggtaa gatattcggg ccaatggcgg tagtaactaa atctgcttca gcgatttctt
      961 ggattacttt ttctggattt gtttgattgt tgattccttt gacatttttt acagtgattt
     1021 tttcttttga ttcatctgct aattcgattt cgtattcttt tctttcattt aaagcatcaa
     1081 tgatcgtgtg attcacatct acgaaagtaa tctcaaatcc attagctgcc aaaacctctc
     1141 caataaatcc tcgaccgata tttcctgcac caaaatgtac tgctctcatt tttctctctc
     1201 cttaagcaat tgctagattt tccgttactt cttctttgct gagcgcgtct gctagctgga
     1261 caacattgtc catatcactg caataaagtg cgatttgctg tacaagttgc aaatgctcat
     1321 ctccgactcc tgcaataccg aacaaaacag tcgcaatctt ttcttcttct tcggtaccaa
     1381 aatctacacc atctggtact tgaacgacac agatgccgga ttttttcaca tatttttttg
     1441 cttcatctgt tccatgagga atcgcgataa aattccccat ataaacagat aacattttgt
     1501 ctctatctac catggcatcg atatattcct catcaacgca ccctgcttct acaagcatct
     1561 gaccgcaata gcggatcgct tcttctttgg ttttaaaaga tcgattgagc tcgatcatgt
     1621 cgaatggcaa actttgcatg ctgttttcct cccttaagct gtctgtttca aatattgtgc
     1681 aatcgattcg tatttttgcg gattgaccat gctgttgact gataaatgaa tggattgagg
     1741 tgcagccatc tgtacttttt ccgctaaact ttctttggct acgatcagtg aatcaggttc
     1801 atcttttaaa tcttggatat tgacttttct taacgggata gttaaatggt tcttgtctaa
     1861 tatatctttg agcacggtca tccccattgt tgctgatccg gaacgatcat cgtggacaag
     1921 tagaatctct ttgatttgtt tgatgtttgc tgaaagaaac tcttctgctg gctcattctc
     1981 aatttcagaa actgatgttt tctgcttgct gcttaattct tgaatgattt catcatatcg
     2041 aggtgaattc aaaaagtttt ctactgagac aaaacgcgcg ctcggtgctt tttgttttgc
     2101 tctttcttgt agttcgacct gagtaacgat caatgtattc gagtcatctg tcagttggct
     2161 gattgcttga ttggtcaccg tttgaggcaa gtctgcttcc tgtacttttt ttctcaagat
     2221 cgaagcgccc atcgcacttg atcccattcc tgcatcacaa gcaaaaatga ttttttgtac
     2281 gccagagaat gtagctcctt gaggaatacc ttgggcacct ttggaagctt gcttagctgc
     2341 ctttgtctct tgaacagatt tttcgaagtc ttcatttgta tctttcgcat ccgctttcaa
     2401 aattactgca gaaataccaa acgaaacagc tgcggcaaca atcacgccca aaataacagg
     2461 aaggtaagaa ttgaacggtg tcatcgctag aatcgcaatg atcgaccctg gagaagctgg
     2521 cgctcttagt ccagcatcca tcaattggaa aatgaaactt cctgacattc ctccgcctat
     2581 aactgataag aataataacg gtttcatcat aacgtaagga aaatagattt catggatccc
     2641 gcctaaaaag tgaatgatca tggctcctgg tgctgaagat tttgctgctc ctttgccaaa
     2701 caaagtaaaa gcaagtaata cgccaagtcc tggaccagga tttgcttcaa gcaagaatag
     2761 aatagatttt ccagcctctg ctacttgttc agttcccaat ggtgtcaaaa tcccatgatt
     2821 gattgcatta ttcaaaaata agatttttgc tggttcaata aaaatacttg tcaatggaat
     2881 caaatgggca gataaaatga tctctacacc ttttgccatt gcttgagtca atgcatcgac
     2941 aactggacca atcgcagaaa aacctaataa cgctaatcca aaaccgataa gacctgctga
     3001 aaaattattg actagcattt caaaaccgga tttgatccgt ttttggaata gctgatcaaa
     3061 tttcttgatg gcatatccac caagaggtcc catgatcatc gcgcctagca tcattggaat
     3121 atctgttccg acaatcacac ccatcgttgc aattgcccca acaactgatc cgcggtcacc
     3181 agcaattact tttccgccag tataaccgat caataaagga atcagataag ttagcattgg
     3241 gcttaccatg gtagccaaag cctcatttgg taaataacca tctgggataa atagagccgt
     3301 taatacaccc caggcaatca gtgctccaat atttggcatg accatgcttg ataaagtact
     3361 gcctaatttt tgtaccctcg cttttagcga aatgttgttt ttgggaatcg tccgttccat
     3421 tttgtctctc cctctctcct tttgatactc taattgtatc cgcttactaa aaagagaaat
     3481 agtcgtactt tggcacgaaa ctttgtgcca aaggtttgag tatgtcagca aaataaaaag
     3541 atttcggtat cagaaaactt caattttaaa taaaactgtt attttaattt ttttataata
     3601 tgcaaacaat aacttttgtt agcatttaat ttagatgttt tccattctat tatgttatta
     3661 aattttatat tggaggccta tctatgacat tactgatcaa aaacatgaac atgaaacgtg
     3721 gtaaaaaacg tgtgattcga caattgaatc ttttagtaga atcaggagaa gtagctgctt
     3781 tgattgcccc gaacggattt ggcaaaacaa ctttgctgaa tggcattgcc caactgctgc
     3841 caacaactta tgatcaattg tcccttcaca acatctctcc tactgcttat acagattatc
     3901 gccggtcttt cttttttcta gaatcaacga atcagttatc cgactatcta tcggctgttg
     3961 attatttgac tttgatcaaa tactattggc agagcaaagt agaatgccaa actgttttcc
     4021 aacgattgaa gatgactgag taccttactt tgccgattgg aaaaatgtct ctaggaatga
     4081 aacaacatgt cttgcttgct gcttacttga tcagtgattc atctgtcatg ttattcgatg
     4141 aaccgttgaa cggattagac ccaggaagta ttgatatctt gaatagcctc atcagacaat
     4201 ggcagaaaga ggggaagacg attcttcttt cttcccacgc tatcgcgaat atccaatcga
     4261 tttgttctaa agcatgtttt ctaaaaaatg gagaaatcgt cacagaatcc tcaaatatgg
     4321 aagagatttt agctatttat cgtaaattat atgaggtgta actatgtctt tacttacatt
     4381 tgaacgcaat ctcttactaa aaaacaaagt aaacttttta tttcctctat tgttggtcgt
     4441 gctctttgca ttcccacttt tttttgatca taaacttgct tatacagaat tcgatgaact
     4501 gaatcataac tatgaagaga tgcaacgttt gattgaaacg ttgaaagaag atgagaatga
     4561 gaaagaattt gtggaaagtt tagagaaaag caacaaactg atcgaagcaa tcttacatgc
     4621 aaaaaacaca gggaatgtcc agcaaatggt cgaagcaact tatcattatg aaaaagatat
     4681 actggatcga ctgatttctg gtcaaagaca aggaattcca atcattgaac aacaaaaacg
     4741 tgtcgagttg ctccgttata tgaaagaaca tcagatccaa agatattcta tttttgactt
     4801 gccagcgcat ctccctttag caaattacta cgaaaatatt ttttcaggaa tgatttctag
     4861 ttttttgatt ttatgtataa ccgccttgtt tctttcttcc atcatctctt atgaaaaaag
     4921 aaagcaggta atctctctag tcaatctatt accggatagt atggtaaaga aacatagcat
     4981 ccgttttacc atttattatg gtgcagctat gctaagtctc gtgatgccat ttttgatcgt
     5041 ctctatacta gtaataatca aaaatggttt gggagacttc cgttatcctg taggaacgat
     5101 cattggtcag gagatacgaa tccttccgat gtatgagtat cttttccaga gctttctgtt
     5161 cctgttgttg tgggtgctct ttttaagtac aatcagtttt cttctatctg ctttatttga
     5221 acattctcta gtcaatcttt taggtacact tctttgtctt ttcttagcag agtatcgctt
     5281 gttttcttcg attggatgga tagaatcaat cagtcattac ttgccaactt cttatgttga
     5341 ttttcaaaat gtgatcatag gaggagatat tttctcgcca ctagcttcag aacaggttac
     5401 tttcatgaat ggtatcttga cacttggtat atggagtatc gttttattat tcataggtat
     5461 gggcactatc tatataaaaa agagctatta aaacacaaaa ataatagggg cggtgacaaa
     5521 aaccttgtca ccgtccccta tttcaaataa acggttttct agaataaata gttttcaaaa
     5581 gcttgcatcc gcagtaataa gaacctccca acaaaaatag ctcctcttat ttttatcttg
     5641 gttgaagtta ttacttgatg caaggcagct ttttttactg tgaacattgg ttatatatgt
     5701 ccatgttcaa aaatcatttg gacttcttca atggttttcg catcactgag cgccattact
     5761 tggtcgatgt ctgaacagaa aaaggctaaa tcttggagca ccgctaactg atgttctttt
     5821 tttaaagcca ctacaaataa gagtgtcgcg atttttggtt cctcttttgt tccaaagtcc
     5881 acaccatcag gcacttggac taaatagatt ccttcttcta ctacttcttc atctttgtca
     5941 gtcccatgtg gcaaggctac gaaattcccg atatagacac tcgctttttc ttgtctttcg
     6001 atcattgatt ggatatatgc cggtttgatt cccccttgtt gggtcatcca ctggccgatc
     6061 tcggtcaacg cttctttcca attttgatag gattgatcga gcaatatttt ttctgctggt
     6121 agctgtatca tcttttcacc ttcatttctt tatttcttca atcaggattt tcgctaacaa
     6181 ctgataaacg atttctttat ttcctgagcg gaagatctcg gtgttcaagt cattcatgat
     6241 gattgctcca ctgatctttc ctaaaagaat cccgtctttt tcttggattg gcgaaggcgc
     6301 cagcataact aacatacggg acaaatcgac tggctgtcta tccatccctt caatggtcaa
     6361 cggatgtttc aaattgaaaa tacaaaagat cggtttagaa atcaatgagc tggatgtatg
     6421 gaataacccc atatttgtat gaggaatacc gataggtgct tgttcatatc gcttaagcag
     6481 ccggcgatga accacctctt catccgttac tagtccttca ggaaaataag gaaataagct
     6541 gcgaatcgtt tcggaaatcg tctcaggatt atccagttct tggataaaaa aacgaccgag
     6601 taattgattg atttgatcca tgaaatgcat tgtttcctca taagtatctt ctatcatcca
     6661 ctcgttattg ctgttatacg taattttttg cctttttttc ttctcaatat gcgaaaaagc
     6721 ttcttttagc tgcttgactt catcttctag cagtagcggg gagactaaaa tatattttcc
     6781 accgtatcct ggtaaaatag atgtagaaat aacaacatca tattcttctt gtaaatcgat
     6841 tttttttaaa tcagcaatcc gatagaaatg aatttgttgg atgaagggaa aatacctttt
     6901 caatcgtagt tccaacatgc tagtagaagc taatccactt ggcgaaaatc ctgcaatact
     6961 gatattgctt tctttcggac ttcgttccaa tgaattcgca aaatgaagga ccatataagc
     7021 gatctcttct tcggataatt ttttttcttg aaaaatccgc tctactgctt tacggattgc
     7081 attcgcaact tcctcgtatt gaaccatgat tttttctaaa atcggattgg tcagtgtctc
     7141 ttcttgtaaa acggctcttg aaaacacacc agataaatga gtcaacagca ttttgtacaa
     7201 agtttcatct tcataaaaat ggacattcgt ctcttcactt acttcttgaa tcagttgttt
     7261 tactaaataa gcaagctgtg tatcgaacgt ctcttcaaag aaattctgat caaatgagtt
     7321 cgtgaagtca tttaataatc ctgcaaaaaa gacgatctca tttactggat aaagttgttt
     7381 tgtctcagaa gcgatccgtg aaaacaatcg tttagctgtc tgtagtaatt ctttgtttgc
     7441 cagactatta tatgtctcat ttgtcagtaa atgattttta gtcacacgct ccatcgtcaa
     7501 tgtcagcatc aaaaccaaat gttcaagctt tctatcactt agattggtct ttttttggat
     7561 caattcttcc ccgattactt tgcgtgcaag agatagatgt tgcggatcaa cgaatcgaaa
     7621 aaaataattc agtgatgcat cgaaagaaat actatgaaaa agttcatatt cattgatttc
     7681 taattctaat acattcgctg tcagaagccg gcggtatttt tccggtcctt cgatctcgta
     7741 tccctggttc cgaatgattt ccaaaggcag ttggcgaata ctttcttcca actgtttgat
     7801 atctgcataa aaagtggtat tactgatgcg atatgattcc atgaaatgag caagactcaa
     7861 cggctctgtc gtcagtaaca actcggctaa aatcgcgtgt tgtcgtttag cagtggataa
     7921 gtcattactt ttcccatctg tattttcttt taattgctct aatacttctt ctgatgcctc
     7981 aatgacaaat gagcctctag ctgctttttt caaagaagca tttaccgatt tcaatgagac
     8041 ttcaagatta tctaattcgc ggtaaatagt ccgtttactg accttcaata aattcatcat
     8101 tttttggata gatacaggtg acgggctact gagaaattct gctaataatt gtttttctct
     8161 gggcgataaa tacatcttat atttctccct tcacgagttt ttcgtattca ttcgtagcta
     8221 cccaatgttc aactagaact agccgaggga tcttttcttc tagctgttca gttacagcta
     8281 gttctctttg tgcgatataa atcgtttctt cttggatttt gattttttct attgattctt
     8341 ttgagataac aaactgtttt ttcgcttttt ttgctaattg acttaaaatc gtcatgccca
     8401 tagtttgtga gccgcgtctg ttgtttttat acaaaaatac aatattagca ggcgactttt
     8461 tttgttctgt gatatctgct atctcttttt tttcttcccg ctttggaaat tggatcgctt
     8521 cttttattcg tgacagcaag atatctgttt ccaaaaagtg atccagtaca tagatttgac
     8581 tttgaggtac tttttctctt gcaagacccg ctagttcatg ttgcacgata accagctgat
     8641 ttacttggtt attcaattca tagatagagc tgtatccgac agaaagatcg attttttctg
     8701 ctgctaattt ctttttcagc agggcggctc ccatagcact tgatcccata cctgagtcgc
     8761 aagcaaaaat gacggaatga atgatagatg gttccaagat ttctcccctt tctttgaccg
     8821 ttttgttctc tttttcttta tttggtaatt tcaatagtat cgctgcacaa atgaaggtca
     8881 caaatgtact gattgctatc cctatactta ccccagctag ttgtggataa ggcgcattgg
     8941 caaaaatcac gagtagtgac ccaggcgata caggtgcagc taatcccgta tctaaccatt
     9001 gaaatatcaa cgtgccgctt gccccaccag ccattacagc taaaaataga tacggtcgca
     9061 gcaagacaaa tggaaaatag atttcgtgaa tcccgccaat cgcatggatc atcaatgctc
     9121 cactcgcatt tactttttcc tgtttttctg caaaacaaag gatagaaagt aagatcccta
     9181 aaccaggtcc aggatttgcc tccatcaaaa ataaggaaga gtacccgact gtttgtgaga
     9241 attcgattcc taaaggtgtc aatacaccat gattgattgc attattgaaa aatagtactt
     9301 ttagaggttc taagatcaga ctgacaagtg gtaacagatt cattgtgact agccaagaca
     9361 caacggaggc gagcaacaag tgaacactat ttaaaactgg ttcaataaaa acgattccaa
     9421 ttatacaaat aacgctacca gtcagtccta ctagtaggtt gctgacaagc atttcatatc
     9481 cagccttgaa tttcacgtgg aatctagaaa caaattgttc atatacgatg ccggcaagcg
     9541 gcccgataat catcgctcct acgatttgtg gagcattgga acgagcgatc aaccctaaag
     9601 aagcaattgc accggcgact gccccttttt gtccccctac cgatttcccg cctgaataac
     9661 tgatcaagat gggtaaaagc agctggatca tccaatggca tacctcttct aatgtggtgt
     9721 gcaaatcgcc agacacacct tttgttgcta atgacaagat tccccaagcg ataaacagac
     9781 ttagatttgg catgatcatt tcatttaagt gatgtccagc ttttctcagt tgtttcatag
     9841 acacgcgact gctgttgttt gctttccatt tgtattccat ctttttcacc atacttctat
     9901 ttattgatgg acggatttta tgtagaaaaa gcagcaaacg aaaaaccgtc tgctgctttc
     9961 agctatctgg gaactatttg cctggttcag tcgcaatgat tgctagttca ccttggaccg
    10021 cccactcaga aacgccattt ggaataatac agcttgttcc tttttctaat ggatatgttt
    10081 ttccgtcaac gacaaggtct cctgctcctt cgattaccgt catcaatgta taaggggctt
    10141 gttttttgaa agatgtgatt cctttaatat cccactcata tacattgaaa aactcagttt
    10201 ctaaataagt gacgatcgat gaattccctt ttcggacttc tttgatttgt aattctggtg
    10261 ttttcgcagg gacagttgtt acatcgatcg attgttggat atgcagctct cttgtttgtc
    10321 cttgcgcatc ttttctgtcg tagtcataca cgcggtaagt cgtgtcagaa ctttgctgtg
    10381 tttctaaaat catgattcct ttaccaatag catggatcgt tccgcttggt acataaaaga
    10441 aatcaccttt tttaactggt actttcttca aaaggtcatc ccagcgccca tctttgatca
    10501 tctcagctag ttcttctctt gtctttgcat gatgaccata gatgatttct gcaccaggtt
    10561 cagcatcgat gatataccag cattcagttt ttcctaattc accttcatgt ttcatcccat
    10621 aggcatcatc tggatgtact tgtacagata gatcatcttc tgcgtctaag atcttgatca
    10681 ataatggaaa cactggttct gaaggatggc caaacaattc cttgtgttct gaccatagtt
    10741 catctaatct tttccctttg aattcgccat tttctactac actgacacca tgaggatgcg
    10801 cactgatcgc ccaatcttct ccgattttat cgttagggat atcaaaacca aagactgaac
    10861 gtaaacgact tcctccccaa attttttctt gaaaaactgg ttttaaaaac ataggttcct
    10921 gcataattca aactcctctc atttttcata gctattatag cattatttcg gtaataccgc
    10981 tttctctttc aaaaagtttt caacaatttc atctcgacat atgagatact ccgcacgatg
    11041 atcgacagga tgtacgcgat ttgacaaaaa tatcaacgct tcttgtgttt ttatatcaat
    11101 caagaaaaac gtacctgtat aaccagtatg aaaaagaatc gcatgaccgt cttctacatc
    11161 cttttttaag tcccatccta aagaacgata gccatttttt ttcggtgttt gatcttgcag
    11221 taaagaatca atcgtctctt cttctaaaaa acgaccatta cggatttttc ccttgttcaa
    11281 atacatttgg acaaaagtaa tgctgtcttt caaatcaata aagagacccg cgttacctgc
    11341 atgttcagcc aaaactcttg ctttcggatc atgagtttgt ccacgcaaaa cctcacccga
    11401 aggcagttgt tctgtgggaa cgattttttt ggaaggaaaa ggaagaaata agctgttcat
    11461 cattcctaat ggttccaata cttgttcttg aaagatttcc actgcagttt cttggtaaat
    11521 ttcctctagc atcaacccaa gaagaatcgt accggcatct gtatatttta cttcttttcc
    11581 tagttgctca cctggttcta gcttcaaata agcggcgatc aattcttcct gagatagctt
    11641 atctctgttt ggtatccatg ttttgatatc agaagtatgt gtcagtaaat gacgcaaagt
    11701 gattgtctga ttttcaaatt caggaagata tttatgaagc ggttgatcga gttctatctt
    11761 tccatcttct aatagacgta aaactaatga agtggttgca acgactttcg tcagtgaggc
    11821 tgcatcaaac aacagatctt ctgtcatcag ctgcttttca ggaacgactt gggcaaagcc
    11881 ggctgtaaat gtttctactt gattttcttt tatgaaggcg actgctacac ctggataaaa
    11941 tccttcttcc atctttttca aaataagctg tttcgtgtgt ggatacatct ttttctcctg
    12001 cttttctatc agattcttac agtccaagct taaagcttaa tctatgagga atcaaccttt
    12061 tttacacaaa aaaactgcgc gaaaaaccgc gcagtttccc ccggtatact attttctctt
    12121 tctatctcta gaataagcga tagtatgacc gttcataaaa tgacgagaca tgcgacaatc
    12181 gttaagaaag aaaaagttaa gcgtcgcatc gaatgtcagt tttattcttt tcttttggct
    12241 cactgacgaa ccaccattcg attcctatag catcatagat tgtgagtact gtgtgctttt
    12301 tgtctaagaa gaaatcaact gatagttgct gcaagtgagc aatcagtcct tccatgcttt
    12361 cagtatcgat catgaatttc agaaaatcaa gtccaatcac ttcatctgta ggcaaatcaa
    12421 ttgcgcctcc gctagcttct gtcaaaccaa catggaaatc tccttcattc aatacatgga
    12481 tgccctcttc ttcattatgt accttcaaac caagtacatg attaagaaat tggcgttctt
    12541 ttgttaagtt agtaacattt aaatggattt tttcaaaata agaacctgtt gatagtttac
    12601 tgaatttttc ttcagatttt cttaataatg cttcttgatc cattgtttct ggaatttctc
    12661 tttggtcgtt cttattctct tcatggtaaa tttccaattc attctcttct gggtctacga
    12721 gcaaaatccc catacgtcca tgatcttcta aagcttcttt gattggataa ttcattttac
    12781 ggatacgaca aaggatatct gccatttctt ctgtgctagg gataatcaat gaaaaacggt
    12841 tcagcttttt gatcaccccc atgtgatcat tcgctctagg actttcttcc agcagcaaca
    12901 tttcacttcc agcttcttct gttcctaaaa tggctaattc gttttcttct cttttcaaat
    12961 caaagccaac aacgttcttg taaaaatcaa tcattttatc tcggtctttc acccgaatgg
    13021 ctacactttt taaataggtg gcagccccca attgaaaatc tttcatattt cttgctcccc
    13081 tctacgtcaa gctttatttt aacgatggat tcttcttctt tcaagcaata actttgattt
    13141 tgttaaaaaa aatgtgagaa aattcgcatt ttgcttgttt tttgtaaatt aatgagtaaa
    13201 aactcttata aaaagaggtt gtgaaaaagc tgttctgcat caagtaataa gaacagccaa
    13261 acaaaaatag ctcctcctat ttttcgtttg gttgggctta ttaccgcaga tgcaagcttt
    13321 tgaacaccgt ttaaggaaga ggttgtaaaa tcagcggttt gacctgagta ttagagatac
    13381 aaaacggaaa aatagcttct catattttgc cgttttttga gctaataccg aaggacagct
    13441 gattgaacac cgtttattcg gaaaaaggga ctgtgacacg acttttgtca cagcccctaa
    13501 aaaaaataat aaatttgtac tattctatga agcttcttct tcagaaaact gactgttata
    13561 aagatctgca tagaatccgt ctttttccat caatgtatcg tggtttcctg tttctacgat
    13621 cgaaccatga ttcatcacaa taatgttgtc tgcatcgcgt attgtagaca agcggtgagc
    13681 aacaacgaaa cttgtccggt tttccaatag gcggttcatc gctttttgaa tcagtatttc
    13741 tgttcgtgta tctacactag atgttgcttc atcaaggatc aggacatctg gatttgctaa
    13801 gaatgcccgc gcaattgtga ttagctgacg ctgcccttgc gaaatattgc ttgcatcctc
    13861 gtttaataca gtgttatatc cttctggcaa tttacgaaca aaatcgtcta cgtgagcagc
    13921 tttagcagcc tcaatgattt gttcgtctgt tgcatcgtcg ttcccatatt tgatattgtc
    13981 atagatactt cctgtaaaca gccaagtatc ttgcagtacc attgaaaact gtgcgcgcaa
    14041 ttcatcacgt gaaagatcac gggtatctac accatcgtaa cgaatactac ctccagaaac
    14101 atcgtagaac cgttcaagaa ggttgatcag tgtcgtcttc cctgcaccag tcggacctac
    14161 aatagcgacc atctcaccag gtttgacatc caagttaaaa tcagtcatta gcaagttatc
    14221 tggtgtgtag ccgaattgaa cattttcaaa acgaactttg tatggtgtat ctttttcttc
    14281 tggaaggcca gatttttcat tggacatctc ttcttcatcc aacacttcaa atacccgttc
    14341 ggctgaagca actgttgatt ggatagtgtt catcaaactt gcaagttgag tgattggttg
    14401 agagaactgt gttgtatatt gcaagaacgc ttgaacatcc cctaatgtca tgttcccatt
    14461 tgcaactttt actccgccta gtacagcgac gaacacatag cctagatttt taacgaaatt
    14521 catcaacggc atgatgattg ccgaaataaa ttgagctttc caaccagcgg catacagatt
    14581 ctgattttct ttttcaaata cttcctggtc tttttcagta tgattgaatg ttttaacaat
    14641 ggtatgacca ccatatgttt cttctacttg gttattcaat aaacctaaac ttttttgctg
    14701 atctgcgaaa aacacttgtg atttcggcgc tacgatcatc acgatgatca agcttaaagg
    14761 gatcatcaat aacgcgacta acgtcaattg ccagctgatt gaaagcatca tcacgatgac
    14821 cccaatcaat gtgactgcac tagttacaaa ttgagtcaaa ttttgctgca gcgtgcttgc
    14881 gatgttatcc atatcattga ttgctcgaga catgatatcc ccatttgtat gtgtatcata
    14941 ataggaaatc ggtacacgat tcattttggc ttccaaatca tgacgtaatt cataaactgt
    15001 tcgttgggaa acacgtgtca taataaattg ctgtaaaaag ttgaatacag cagaaatcaa
    15061 atacatcacg attactgtcg caatgatctg cgcgatttta tcaaagtcta tcggaaattt
    15121 tgttacttgt tggccttgtt gcatctgttg agcgccagcc attacacctt tgaatatttc
    15181 tgtggttgct ttccccaaga cctttggtgt ttggatttgg aagatcacgg ctgctattgc
    15241 tagtacaaat acagcgatga tcgcaaccat acgtttagac atatagccaa acaatcggcg
    15301 taccgttccc cagaaatttt taggcttttc accttttaca ccgagatttc tgccaggtcc
    15361 accatgtcct ggaccgcctg ctggtccatg agagggacgt gtgtttgttt tactcatgct
    15421 aagtcctcct ctcttagttg tgaatgaacg atttcttgat aagtttcgtt tgttgctaat
    15481 agttcttcat gtgttccttt gccaaccagt tttccttcat ctagcacaag gatcatatct
    15541 gcatccatta ctgtacttac tcgttgagca actaaaacgg tgatactttc tttcatattc
    15601 gttttcaatg cttggcgaag attggcatcc gttttgaaat ccaacgctga gaaggaatca
    15661 tcaaacacat agatatctgc tgttttgact aatgcccgtg caattgccag acgctgtctt
    15721 tgtccaccag agaagtttcc tcctccttgt tccacatggc tatctaagcc atcttccatc
    15781 tctgaaacaa aatctttagc ttgtgcgatt tctaatgctt tccaaatcat ttcatctgtt
    15841 gcatccggct taccatactg catattgtct cggatcgttc cagagaacag aactgctttt
    15901 tgaggaacaa agcccattaa ttctcgcaaa ttatattgag tcatgtcact aatgtccgtt
    15961 ccatttattt ggatcgaacc agattcgatg tcatataatc gagggatcaa attgactaag
    16021 gtcgttttac ctgaaccagt cccccctatg attgcgacga tctcgcccga cttggcttgg
    16081 aaatcaatat cttccaatgc aagtttttct gctccatgat atcgataatt gacatgattg
    16141 aaggccaatg ttgcgttttt tccagcaaaa gaaactgttt taggattttc agggtcatgg
    16201 ataccgattt tttcatccaa cacttcatta atacggtcag ctgaagcttg tgcacgaggc
    16261 accatgataa aaatagcgga aagcatcata aagctgatca agatctgcat cgcataagtc
    16321 ataaatgcaa tcagattacc gacttccaaa gtcatatctg ctatgtagtg tccaccaaac
    16381 caagtgattg ctacgtttgt accgctcatg atcaatgtca tcattggcat catcaatgcg
    16441 acgatcgtat taactttgat tgccgtattt gtataatctt tattcgctaa atcaaaacga
    16501 ttttcttcaa agcgtgtctt atcgaatgct cgaatcactc tgacccctgt caatccttct
    16561 cgaaatacca ggtttaaacg gtcagttttc ttttgcatac ttttgaataa tggaacagca
    16621 aagtacatga ttccgccgat caaaataatc attactggga tgacaactaa gaagattttt
    16681 gtcaactgat gatctttttg ataagcaagt atgctcgcgc caatcaacgt gattggtgca
    16741 ttgatcatca tacgtaaaaa catttgtgta accatttgga tttgagtcac atcattggtc
    16801 gttcttgtga tcaaagaggc cgttccatat ttatcaaacg catcttttgt cagactctct
    16861 gattttttat agacatccgt acgcaattgc ttacccagtt tctgtgattc acgagtagca
    16921 aaaaatgtat ttgcgatagc agctaagata ctgatcaaag aaaagccgat catcacaaaa
    16981 cctgtatgcc aaatataatc gacatctcct tgcgctaccc ctttatcaat aatattggag
    17041 gtaagtgtcg gcaaatataa atctgccagt acttggatca ccatgaaaac tgcagctgca
    17101 attgctgaag tcaaggagat ccgttttacg atttttagca tctagttccc ttctttctat
    17161 ttatatactc cgtttttcag ccaagaaagt ttcaaattga attcactgat tgcttgctct
    17221 actgagtaat cttcagatgt tgaaaataag cgatacgaat gcgcaattgt tgtaaataca
    17281 gtattcatca ctaattggat cagtaattcc aattcatgat cattttttac tttcagcttc
    17341 gtccgatcga tcaattcaag caactctttt gcatcttgtt tgtgttcttt atgtggatcg
    17401 tgaggcgttt tttggcgact cgtatgagga gcgacttttc ttgaggcacg gtaatccatg
    17461 ttcataaaca agttacgata aaaagaagca ttctcgcctt ccaaaatctc ataaatcatt
    17521 ttagaaaaat aggattcgaa tcctttgaat agatcaccat cggattcttt cattagttgt
    17581 aacatatctt tagagctgtc acgtctcatt gtctcaaagt aataaaaata aagatcctct
    17641 ttatcttcaa aatactgata gaaactgcct ctagggatat ccgctaattt gacgatatta
    17701 gcaatcgaag cttcttttaa gggtactcta gaaaactcga ttctcgcagc ctcgattagt
    17761 cttttttgtt tttctttagg caaatggaaa aatgtctgtg tcggcatatg gatcctcctt
    17821 tctttcaaga tgacacattg tcatatgacg ctgtgtcatt ataacacctc ctacctgttt
    17881 tgcaagacat ttgcatagtt tttttcatta tcatccgata cattaaaaaa gtgataagag
    17941 gaatccttcc atttgaatta atgagagttg cccattaaaa tagccacata ctaaacaaag
    18001 gatgagataa atggcccaat caattagtgt ccaagacttc aataaattgc ctctcgatgc
    18061 tgacagcatc gttttagata caagaaatac cttagattac acaaaaaacc acttaagcca
    18121 ctcgctttct atccccgctg ctatcttgcc aagacaatta aaaaatctag atccaaagaa
    18181 actttattat attctaagct atacaggcag gcgatcagaa gcattagccg atcaacttac
    18241 tgctaatggt tttcgtgctg tttatatcat tggtgggatg gaagctcttc agtcgagtgt
    18301 tgcttgaata ttgcttgtta gattcaaaag aggctgtgac aaaaaatcgt gtcacagcct
    18361 cttttatacc ctgtcttttt ttagttattt attcttcaac cttatcaata tgacttgata
    18421 gccttttctt tttctcatag ttataacaat catagcaata aaaggagaag gtaagcatat
    18481 ggcatctgtt ttagtagtca aaggtcatcc cctaactgca gaagaatcgc gcacagttaa
    18541 ggcattgacg tcttttttaa caagttataa agaaaatcat ccagatgatg aagtgactgt
    18601 tttggaactt tatcgcgatg atattccgga aatcgatgaa gaattattat caggttggga
    18661 agcattacgt gctggtgcag aattcacttc cttatcagaa agtcaacaac aaaaaattgc
    18721 tcgtttcaat gaattgactg atcagttcct tgctgctgat aaaatcgtta tcgcaaacgc
    18781 cttatggaat ctaaatatcc caacaaaatt aaaagcttgg ttcgataccg tcaatgtagc
    18841 tggaaaaacg ttccgctata cagaaaacgg gccagaagga ttagtgacag gtaaaaaagc
    18901 actgcacatc caatctaatg gcggtgtata caacggacaa gactttgcct ctcaatatgt
    18961 aaaaggtatt ttaaactttg taggcatcga tcaagtagat cagctattca tcgaaggaat
    19021 cgattatgac cctgaccgtg cagatgagtt gatgcaaaat gcattagata aagctgctgc
    19081 tttaggtaaa tcattttaaa aattgagata aatgcataca aaagagacag aggaaaaaat
    19141 cttcaaagca gcatttgtac cttggatatt ttcccatctg tctctttttg ttattctttc
    19201 tgctttttca acggattttc ttcataaaga gaagagattg ttgtgtacca ttcaaaaaca
    19261 tgagtaatca ctactttagc tgctgcataa acaggaatcc ccaagatcac accgatgatt
    19321 ccgaacaatt tcccagaagt cagcagaact aataaaatcg ttactggatg gattgctaac
    19381 tgattcccta gtactaatgg agagatcaag cgtccttcta ttgtctgctc tactgcaaaa
    19441 acgatcagta ctttgatgat catgaatgga cctgctacaa tggctaaaaa gatagccgga
    19501 atcatcgcta aaaatgatcc taagtaaggg atcaggttca aaaaacccgc aataatcccc
    19561 aacgtgacag catactctaa accaatgacc gcaaaaccaa tcataaacat gattgccaca
    19621 gcaaaagcca cagttagttg tcctcttata taagaagaaa cttgactatt catttcactc
    19681 agcacattca acgtcggttt ttgcatgcgt gtcggtaaaa atttgacaaa ataaggtgct
    19741 aattgtttac cgtctttcaa tagataaaat aagataaacg gcatcgtcag cagagccact
    19801 aaaatagtcg ctaccgcgcc gacaaaactt ccaagactct gaactgtact cttagatata
    19861 tcttggatca tatcgcctgc cgaattcatg aacttatcac ctgctgtttc caactgttcg
    19921 cgaaattggt tgaacagcgg gtcccgcaaa atctcggtta atttattatc aatcgtatct
    19981 acatattgag ggaagttact gatgaagctc actgtttgtt cctgaatctt agggatgatc
    20041 actacgctcc cccatgcgat tagtgcaaca accaaaacaa acagcccaat gatactgtaa
    20101 agccttggta cttttcgctt ttcaaagtaa tcaactaccg gattcatcaa gtaatacaag
    20161 attcctgcta aaatgatagg cagacctacg attgctaaaa attgccagat tggtgcaaac
    20221 aaatatgaaa cttttgtaaa taaaaagacg atcagcaata ttaacaatac aacaagtaag
    20281 gctgtgacta cttgattatt taaaaaccag cgccaaaacc acgataccgg tctgttcttc
    20341 ttattttttc cttccataat tcctcctgtt agttataaaa aatttggctt cccaaaccaa
    20401 tagcaggcag tctatgcgga tgaagataga ttccatttgc aatttgtcct tcttctggac
    20461 aagcatcaaa aatcaatgat acgtgagcta ctgtctctaa attcccattg gccatcggac
    20521 cgacataatc gatcgtatat acctgatcat caaaagcaat tgttccgcct gctttcaaag
    20581 aaaaaggttc gtcttttgat acttcttgaa tcaccgaata ttttcgtaag gcagctgttg
    20641 ctgttttatc gaacaaaatc agcatgggtt cttccttgtt tattgcttct gtcccaattt
    20701 ctgtaatctt tgcctgaatc attgcactct ctcctttaat tgctttcaaa aacagtataa
    20761 cacattctca ttctttagcc gtgtggaata attatgtgat agaataaaaa cgaaaactat
    20821 ttcattacaa ttaggaggaa tcagctcata tgatcgaaaa gataatttta ggaacctata
    20881 cgcgtcgcga aagtaaagga atctatacga tcgaccttga tacagaaaaa gaagaattag
    20941 ccaacctttc attagctgtt gaagaaaata gccctaccta tgtagcaaaa agtaaagctg
    21001 gcaatcttta cacagttact agtgtggatg gattaggtgg tgccggtgcg tacgataagg
    21061 attttcattt tttgaatgcg gtaacagagt ctggcgctcc tttgtgttat gtagcggttg
    21121 atgaagcacg tcaattacta tacggggcga actatcataa aggtgaagta aatgtctacc
    21181 atatattagg agatggcgaa ctgaaagcag cagattcaat attccatcaa gaagcgacag
    21241 gtcctcataa aaaccaagat catgcgcatg tccactatac ggatctgact cctgaccaac
    21301 gattagtcgt atgtgatctt ggtacagatc gtgtgtatac ttatgatgtg tccgaaaacg
    21361 gcaaattgaa tcttgtaact accttcactg cagagccagg aacaggccct cgccatcttg
    21421 tctttcatcc aaatggacaa ttcgcttatt tatttggtga attagacagc acagtccgcg
    21481 tgctttctta taataaagaa acaggtgctt ttgaagaatt acagaaaata tcgaccttgc
    21541 cagaagaatt cgacggtgaa aatggagggg cagctatccg catttcaaat gatggcaagt
    21601 tcctttatgc ttctaaccga ggacataact cgattgctgt attcgcagtt tttgaagatg
    21661 gttcccacat tcaaaacaag caaatcatct caacagaagg ggattttccc cgcgatttcg
    21721 cactagatcc aagcaataat tttgtcgtgt gtgccaatca gaacaccgat aatctgacat
    21781 tatatcgccg tcatcctgaa acaggattac tggaattact gaaaaaagat gtctatgtac
    21841 cagaatgtgt ctgtgtttat tttgatcgtt agtaagaaaa ggagaatgaa atgaatcagc
    21901 aagacgtatt agacagacta cgggaagaac taaatattcc tttctttgat gggaaaatcg
    21961 aagataaaga gtatagcgaa gaggaatatc agaaactcaa agccgattta cagaactact
    22021 ttgaacaata tgtacagaat gtagaaaact aatcagcttt ccatactatc tgaaaagaac
    22081 atcaggacaa gacttttccg tctatcctga tgttcttttc tacaaataaa aaaccgccga
    22141 gatggttaga ctcgacggaa aaggagtatt tactcgattt tgagtaagta caaggttgtt
    22201 gttggttata ggtatgtcat tatcataaag gattttgatc actctatcat aaaggtaata
    22261 ttacatttat attagataac tctagttttt attgttatca aagatttctt ttattttttc
    22321 aacaacaaat tagtcatttt ttggatggca gctgtcaatt ggtctgtcgc ttggtcttgt
    22381 tttgcttcag atgtattgaa tagatcttca tctacccctg attcaagatc gatactttgg
    22441 atcatttctt cacagcctgc tacatatgaa agataagctt tttcaaaatt tttatgaatg
    22501 cccattgctt ttgctggtgg acgcaaagta ctgactttct taagcattgc ccgatatttt
    22561 tttgttcctt cttcaaataa caggatgatc tcttcaagag tcgatttgtc cagttcactt
    22621 gtttttcctt catcaattgc gtttctgatc acttcatatt tcggatgcat tccttctccg
    22681 attgcttctg tttccttgac gatttgatcg atggactgca cataatagcc aatattcggt
    22741 aacatgttcg ttttcctact ttctccattg ttgttattat ttattctact tgtttttatc
    22801 ctcaaatgac aagtacttga aaaaatttaa tggtttctat acagctcgcc attcttattc
    22861 gtgctcaaag aaaccatctg ctaaactata ttgtttccct gcgacaatgt catattggat
    22921 caattgatat tcgtcgtaaa tctcttgttg cgagcgattg aattgcgctt ctttatacca
    22981 ctgtccgttt tgatagtaca gttctcgttc aaacgccgga agttcctgtt ctaatgataa
    23041 aagcagctga taaaagccat ttgttgtctg attcgtctgt tccattagat tcgctgcaaa
    23101 ataaaacgga ctggtgattg catcgtgagt cgtttgcttt tctaattctc ctttactgtc
    23161 gaacataaga aattgtgttt catgcaatgt ttgtttgtca ttttttgctt ggatcgtatc
    23221 tgaatagatc cctggaagat gatcccccca aaaaacaact aaagtccttc tagaaagatt
    23281 cttcaattct tccgtgaact gcttcagtgc cgtgctgcta taggaaatat cttgcagata
    23341 attctctaag tcaagtgtcc cacttccatc tgaagttttt gcaggatagt ctagattatc
    23401 atactttcca tcgtagggca tatgtgtctg catcgtgact aaatgaatga actgaggtgt
    23461 tttttcttcc ttgagaagtg tcattacttc tttgtaagct gactcatcag aaatatatgg
    23521 attattctct atcgtatccg tataggtcat cgtacgctcg ctgatgaact ggtcaaatcc
    23581 taatgtctga taaacatcct ctcttttata catcgaagta ttgtatggat ggatcgctgt
    23641 cgtctggtat gattgtgcgt ttaacgctga aacgatagaa ggaagctgat ccatttttgg
    23701 tacaagcatc gtgtaagggg tggtgagttg cgcattaaat aacgccatag aaaaaccagt
    23761 caatgcttca aattcgatat ttgctgttcc accgccatag ttttgagaaa gcatattgcc
    23821 actatatgtt tgatcagcta cttcataata atctgccaat ggctcaccag atacttctac
    23881 gccgtttaat cgactaggat ctgagaaact ttcactcatc acgaacacga tgttcggcga
    23941 ctcttcctct acagttttat ttttttcatc ggctagcttc tggtattttt cagtgatttc
    24001 tttgatcttt tctttcgaat atccttcagg ttcatccatt ggttctacct tcagattgta
    24061 caaaaaccca ccgataaaac cagtgttata gtagttcatt ttctggctgt aaggtatcca
    24121 tagtgccgtt ttattatatg cttttcgcaa caaattatcg ggattgttga aattactgat
    24181 ataccctagc agtccaattg ccacaagcag tgtcgttaat cggatcaatt ggaatcgcct
    24241 tccttttttc aggcttcgaa acatgtacca gcaaaacaat cctatcacgg ttcctgcggc
    24301 agctaagatc actgtaaaca gcatagtgcc ggtcatatct ttcagcaatc cgatttcagt
    24361 gatcatcttc aaatcgtctg gataaatcgg ttcttgccga taactcattt tcatataatt
    24421 tgcaaaacct aatacaccga tggctacgac atacgtaagg gaacctaaag ggacagatcc
    24481 tgctagtgca atcagaaaaa taagaataat cagcaagacg agacaggcta agaagaattt
    24541 ttccgtatgc caagaaaaag caaatttcaa cgcaaggtct acggatagat cattttgaca
    24601 ccactgcaaa aataaattac taaaaacaac aagaaaaaag atactgatga ttgctgctgt
    24661 tccttttaaa atctttttct gtttggtcga aagtctgctt atatatcttt tcaaatgctt
    24721 gctccttcat ttctatcaat aaaagaatgt tgcttctctt attttagttg tacgaaattc
    24781 gaaagtaaag aacggaaaga agaattaatg aaatccaaat attctctttc tttatggcct
    24841 ttctggtttc tttaataagc ggacctttcg tactccagaa attttttcta gctgttgttt
    24901 cacttgattg agctgttcaa aatcagtact gtctatgtca atcaatatgt agacatgctc
    24961 attcttatgg ttacgcgata tatctgcgat cgctagctcg ttctcattca gcagagaaaa
    25021 tatctctgct aatatattgg ttttcttttg ataaaatata gtgaatcgaa acggcgcttg
    25081 gaaaaacatt cgtgcggatg gataattgac cgcttctcta accgtaccga acaaaagata
    25141 atcttttaag gcttcaactg ccaaacgtcc gctgtctgcc aaagcttcct gagtcgatcc
    25201 ccctagatga ggcaacataa gtatacggtc attttctaag aactcttctt ctggaaaatc
    25261 agtcaagtat ttagccagat gttgtttttc caatgcttgc ataacagcat atttatcaac
    25321 aatttcactt cttcctacat tgataagtac agcatctttt ttcatttttt ctatattcgc
    25381 ctgatcgatc aatccttttg tatcttctgt caacggcagc atgactacga tgtaatccga
    25441 ggtagaaaga actgtttcta aatctgcttg ccgaacataa tccaactggg catcgcggat
    25501 agaataaccc aagacatcca tccctaagct atagcaactt agcgctactt ttgtgcctat
    25561 cgctccaaga ccaagaagac cgatggtttt gccttgcaat tctcgtccca cataagcact
    25621 tcttttgttt tctgcctgtt ctaaaatatt aggacctgtt agtgtttgta ccatacgact
    25681 tgcttcgatt atcggacggg aagataagag caaacagcac aaaacaagct cttttactgc
    25741 attagcattt acacctggtg tattcatgac gatcgtcccg ttttcagaag ctttttcaac
    25801 gttgatcgtg ttcaccccaa caccagctct tgaaattgct agcaaatctg gtgtaagcca
    25861 ttcatctttc actttcgtac tacgaataac tattccttcg gctggcaaat catctattat
    25921 tttgaaatat tccccttgta acttttctat ctcttgttct ggaatagcat caatcaaatg
    25981 aacgtgatac ataaaacccc ctccttacta acaagtatct cgaaatttct gcaattagac
    26041 aactgtttac catttgactt cctagaagca aaaagagcag ttagagcaga gtaggctgag
    26101 tgataaaaat aaggctatga caagttactg ccataagcct tattttcgag agcaaaatat
    26161 tgagggtgag atatcttgat aaatggtgtt ccgttagctg acattgataa taagactaaa
    26221 tttcaaaaaa gttgaaaagc aaatttcgtc aatctcttct tatttcttaa aactaagtgg
    26281 ctttttcaca acctcttttt atctaataac cactgtttcc aactcaacta attttgccca
    26341 cgtttcaatt tgatctgttg ttagatttag cgagacaacg gtatgatgac cgccacctgc
    26401 ctgaatccat gaatggacac cttcatggaa attcggttta gttttccata acacacgtgc
    26461 aacaggtaaa ttaggtgctt cagtcgttgg ttcaaaggct tctacttcat tgatcagcag
    26521 tttataatgc gtaccgaaat ctgccatcga tacgactacc ccttctcctg ctttcccatc
    26581 aaatacaaga cgcgccggat cttctctatc tcccattgat aaaggagaaa caacgattct
    26641 tggtttcgtc gcagcaagcg tcggatctac ttccatcata tgggattcca agattgcttc
    26701 ttggccacta gctagctcgt aagtataatc ttccatgaat cctgtatctt tccctcgagc
    26761 catgattttc agcaaccgat caatcgctgc agttttccag tcgccttccc cagcaaaacc
    26821 gtatccttct gccatcaaac gttgtacagc tagtccaggt agttgtttca ttccatacag
    26881 gtcttcaaaa ttagtcgtaa aagcgttata gcctccttct tctaagaaac ggcgaatacc
    26941 gatttcctgc tgagcttgta cttttacatg ttcctcccat gtcttttctt cataatcacc
    27001 atagtcgaag gtatacaatt ccttgtactc ttcaaataat tgatcgactt cttcgtctga
    27061 tacgcgatcg attacttgta caagatctcc gataccaaaa taatcaacag tccaaccaaa
    27121 ttgaatctgt gcttccactt tgtctccttc tgtgacaccc acgtttctca tgttgtcacc
    27181 aaaacgagcc acttttattc caaaactttc attataggct actgcaacat ccatccaatc
    27241 agcaatctct ttttggatct caggtcgttt ccagtagcca acgacgattt tattttgttt
    27301 atttaatcga gcattgatga acccatattc gcggtcacca tgagcggatt gatttaaatt
    27361 catgaaatcc atatcgatcg ttttccaagg aatgctttcg ttatattgcg tagctaaatg
    27421 aagcaatggt ttttgtagaa gctttgtccc gcggatccac atttttgctg gtgagaaagt
    27481 atgcatccaa gtgatcacac ctgctacttc ttctcgataa ttgacctctt tcatgatttt
    27541 tgtgatcgta tcagcagtaa ctgctaattc ttgaaggcgg attggataag gcaattggcc
    27601 attttcattc agttcatcga ccatttcttg tgcatgtttc tttacttctc gcagtgcttc
    27661 ttccccatat aaatgctgtg atcctacaac aaaccaaaat tctttttctc caatattcaa
    27721 cataattagc cactcctttt atttgttttg tccgtaatac gcattttttc cgtgttttcg
    27781 taaataatgt ttatctaaaa tccgttgagg cagctcttct gaaaatgaat tgagctgtct
    27841 ggtaaataaa ttcatcttgc atacttcatc tagtacgact gcgttcatta ccgcactttg
    27901 cgcatctttc ccccaagtaa atggaccgtg tccgtgtaaa agtacccctg gaattgctaa
    27961 gggatcaatt ttgcgttctt tgaatgtttc tatgataact tttcctgttt catattcgta
    28021 cccgctgtcg atttcctgtt gtgtcaaaaa tcgagcacaa ggtaccgatc cataaaaagt
    28081 atctgcatgc gtcgtcccca tcgcaggaac atccaatcct gcttgtgccc agatcgtcgc
    28141 ccatgtcgag tgagtatgga cgattcctcc gatttgagga aatgaacgat ataaaacagc
    28201 atgagtaggc gtatccgaag agggattgag tttaccttca atgatatgat tgtccaagtc
    28261 cacaacgacc atgtcgtctg ctgtcatctc ttcataactg actccgctag gtttgataac
    28321 gaaatatcgt gtttcaggat caaaagcgct cacatttccc catgtatatt tgacgagtcc
    28381 gtgtttaggc aagtcaagat ttgcttggta tacttcttct ttcagttgtt ccagcatttt
    28441 ctatcactcc tttttcttgc aattgatgaa tcgctgtttt ttcaatgtcg atcccctcac
    28501 ggtaacgtct cataaatatc tgatagcctg ctatttcttc tgctgaaggt gtgatggtga
    28561 ttccttggtc ttgtccaaac acgtcttctg ccaagaagtt ttctaaagac ttcccatgtc
    28621 gatccatcat ataggctgct aataacgcga ttccccacgc tccaccttct ccggctgtgt
    28681 ccatcactgt aactggtgcc tccatagcag atgctagaat ccgctgtcca acttctggtg
    28741 ttttaaaaat ccccccatgc cccactaaac gatcaatttg gacattctca gatttcaata
    28801 tatccattcc gattctcatt gcaccaaaag cactggctaa atggatccgc atgaaattcg
    28861 ctaaatcaaa acggctacct ggtgtacgta caaataatgg ccttccttca tttactcctg
    28921 taatgttttc accggaaaaa taaccataag aaagtaatcc cccgcaatcc gtatcgcctt
    28981 ctagcgcttt gttgaataat accgtgaata atttatctct gcttatctcc atacccaaag
    29041 aattggtaaa ttcttcaaaa atactgaccc aagcattgat atccgaagag cagttattcg
    29101 tatgaaccat gcctactagt tctcctgaag gtgtagtgac catgtcgatt tccggatgga
    29161 cattttctag ttctttttct aatacgatca tagcgaaggc ggaagtacca gccgaaatgt
    29221 tccctgtccg tttggctaca ctgttcgtag cgaccatacc ggttcctgca tccccttctg
    29281 gcggacaaac cgggattcct ggtctcaaat ttcccgtagg gtcgatcaga taagcccctg
    29341 attcgcttaa cttgccagct tcttctcctg ctactagaac tttaggcaaa atgtgatcca
    29401 agtcaatcgc tacgtgcgct ttttcgatta actgaccaaa aatatttttc atttcttgat
    29461 ggtaggtttt cgttgaagga tcgataggaa acatgcctga tgcatctcct acgcctagta
    29521 ctttctcacc agtaagctgc caatggacat aacctgcaag cgttgtcaaa aaattaattt
    29581 ctttcacatg ttcttcttta ttcaaaatag attgatacaa atgagcgata ctccatctct
    29641 gcggaatatt gtattgaaat ttttccgtta atattcgttc tgcttcttca gtgattgtat
    29701 ttcgccaagt acgaaatggt actagtaact gttccttgct atcaaatgct agataaccgt
    29761 gcatcattgc actgaaccca atcgacccga ttgtcgtaag attctcgcca tagatttcga
    29821 atacttctgc tgccattttt cggtaactga cgcgcagtcc tttccaaata tcttctaatg
    29881 aataggtcca gataccattt tctagtttgt tttcccactc ataactacca gcggcaatag
    29941 gagtaaaaga tgaatcaatc aatacagctt tgatcctagt tgatccaaac tcaatcccta
    30001 aagatgtacg accatctatg acatctaaca tactttgcgc atttacttcc ttcataatga
    30061 tttaactccc tccttctcgt caaacctgat caaaccgtct ttttagtagc gcttacaatg
    30121 attattgtat acttttgtac gtacatgtca agcggttatt tacaagaata caataaaaaa
    30181 cacttgtttt gtacgtacat aagttataaa atgaaattgc ttacaaaatc tgatcaaccg
    30241 ccttctcgaa attctagaag gagagaatac ttatgaaaaa agaaaaaaaa gtttcctcat
    30301 cgtttattta tttttttggc gcatttggcg gcattctatt tggatatgat attggtgtta
    30361 tgaccggcgc cttacctttt ttacagcatg attggggttt ggcaggtaaa gcttctttga
    30421 taggatggat cacgtcttcg gtcatgttag gtgcgatcct tggcggctct ttatctggtc
    30481 ttctttctga taagcttgga cgacgaaaaa tgattcttct ttctgcactt atttttatgg
    30541 ctggttctgt cctttctgca tctgcccctc acaatggttc ttattttctg attgcagcaa
    30601 ggattcttct aggtttagct gttggcgctg catctgccct agttcctgct tatatgtctg
    30661 aaatggctcc tgctagacta aggggaagat tgtcagggat caatcaagtg atgattgctt
    30721 ctggcatgct cctttcctat gttgcagatt atttgctaaa agtgttgcct gaaacgatgg
    30781 cttggcgcgt gatgttaggg ctcgctgctg tccccgcgct gattttattt ttcggtgttt
    30841 tagctctacc agagtcaccg cgttttctta tgcaatctgg ccgcttggaa gaggcaaaaa
    30901 gagtattgaa ctatattcgg acaccaaaag aagctgaaca ggaattcgaa caaatccagc
    30961 tgaatgtcaa acaagaaaag acaaccggaa cttcttggca tactttgttt ttagaaaaat
    31021 atcgttcctt agtcattgcc ggtattggtg tagcagtatt ccagcaattt caaggagcta
    31081 atgctatttt ttactatatt ccgctgatcg tagaaaaagc gactgggcat gcagcgagcg
    31141 atgcattgat gtggccgatc atccaaggaa tcattttagt tgctggttct ttgctctttt
    31201 tggttattgc agacaagttc aatcggcgaa ctttattgaa aataggcggt agcgtgatgg
    31261 gactttcatt catcctacca gctgttttag gaactgtact ggatgctcat acaaattcgt
    31321 tgttgattct cttgttttta tgtatttatg tcgcatttta ttcttgcaca tgggctccac
    31381 tgacatgggt aatcataggc gaaatattcc cactagctgt ccgcgggcga gcatctggcc
    31441 tagcctcttc ttttaattgg attggttctt tcctagttgg cttgcttttt cctgtgatga
    31501 ctgcttctat gtctcaagaa atcgttttcg gcatttttgg gatcatctgc tttttagggg
    31561 ttttatttat tcaagagatc gtaccagaaa ctcgtgggaa atctttagag gaaatcgaac
    31621 aaagtgcatc taaaaaaact tatcctaaac gtatttctat ccatcattct taaagaggtt
    31681 gtgacagaag tggtcagctt caagaaataa gaaggaaccg cttagtttcc agtcacaagg
    31741 aacaatttca aaaaacagct tctcatgttt gatgaatatt gtgacttgtg accagttggt
    31801 aggagaacac cgtttagtgg agaggtggtg aaagaaacgt tcagccttga gcgatgacaa
    31861 aagctctcac aaacattgcg acgagtaacc gtaggagcaa taagaggcgg taaaaacccg
    31921 tctcttattt ttcgttttct ttcactgttt cccgttcaac tagtacaggt tcatagagaa
    31981 tatttggctg tgcctgtcct ttttcaatcg ttgtgacgat ccaccgtgcg gcatcttgtc
    32041 ccaacttttc ttttggatgc gtcaatgttg tcaatttttt attccctaaa tggcttaaag
    32101 aagaatcgtc tgttcctacg atcgataggt catctggaat atgatagcca ttttctgtca
    32161 acttatctag cattaaacta gctatttgat cgttatagca aatgatgcca tctaaagctt
    32221 gttcttcttt caaccgttgt actgtttttt cagcgattgt ttctcgtgtt tccgtggtga
    32281 aagtaatgat atcttccgtc aaaaaacgca cattatactg ttcacacgcg ctgataaaac
    32341 ctttcatcct gtattttcct tgaagatcgt cgatttttgt gatgaacaac agacgctgat
    32401 gtcctttttt tatcaaatat tctgctgcca taaaaccgac tttcacatca tctaaacaaa
    32461 tataagaggc atttaactct tcataagcag cattgatcat caccatcgga atgccttgtt
    32521 ctctcaaagc aacataaagt gctaagtttg ggttatactg attacttttc gtaggctcaa
    32581 cgatcaaacc atctacccca taagcaatca ttttcttcaa acactctttc tccaattcgt
    32641 gatcgttatt cgtactggct aaaagcaatg aatacccaga ttctcgtaat tcattttcga
    32701 ttcctcggat aatcgacgga aaaatataat cagagagata tgtggtgatg acaccgatcg
    32761 tttttgttct gttcaatggt gttttttctt tcttatagct gtcatctaca tatgtgcccg
    32821 accctttttc tttacgcaga tagccctcat ttaccaacac agcgattgct tgccgaatcg
    32881 tatgtctgct gacgtcatat atttcttgca gcttgaattc tgagggaatg gttgtattta
    32941 cttcgtattc ctcacgaagg atcttttgtt ttatctcttc tgcaatatct tgatattttg
    33001 ccatttgcct tgcccctttt tatttgtacg tacatatatc ataacgagtc tgcaaggttt
    33061 attcaagcaa aaatcaaggt ttcttgtgct cataaggcat acggaacagg ttcaccaaat
    33121 tctggaaatc cttgctcatt ccacgtaaag cattgcgcgt tcgcgtgccg attaggattg
    33181 tctaacggat cccctttttg gttcttctct ggtcgagcat gatagatcaa aatatcaaag
    33241 cacccgtctt ctgaaaccgt aaacgagttg tgaccaggac caaattgctt atttttttca
    33301 gaagaaacga atactggctc cgataattta tgccatgatt cttttaataa aagattcttg
    33361 tgctcatcag cccacagcag gcccatggca tagttttcat ctgttgcact accagaatac
    33421 gtaatgaata ttttcccatt gcgagtaagg actgctggac cttcatttac tgaaaaaccc
    33481 cgtttttccc attcatactc aggtatagaa aggaggatct gcttcccctt taacgtccaa
    33541 ggattatcca tttcggatag ataaagatta gaattcccag taatttcagg atcttgttgg
    33601 gcccacacat aatatagctt tccttgagaa gagaaagttg ttccgtctaa actgaaagtt
    33661 tcccttttcg tttttatttg tcccttttct atccaatctg tattcattgg atctatatgc
    33721 tgattttcaa gtacgaacat gcggtgatga tgagattcat cacgtatagt tttatgattg
    33781 cttgcagcaa agtaaatata ccattttcca ttgatataat ggacttcagg tgcccagatc
    33841 aattcactca tcggtccttc ttcatgagcc tgccatacaa tcaggcttgc agcatgttct
    33901 aattcattca gtgactttgc ccttcttaat tcaatcactt ggtagcctgg aaccgatcct
    33961 gtaaaataat aataaccatc tgtatgttta tatacccaag gatctgcccg ttgccgaacg
    34021 ataggattat tataaagtgt cttcatcata acaaatttcc tcacattttc tttattctaa
    34081 ttacattcca agagtatttt tctatcccta catttaattc gtcatgtttt tctattgaaa
    34141 aatagtttat ttcaggtttt attttttctg gttcttcacg tgtatttttg tccaacagat
    34201 cgctactggt taatgtgtag tgctctcctt gatctagcaa attgaaatca tgttcaatcg
    34261 ttatcttttg ttgttcattt gtgcgattga tcaagaaaat agtcagctga ttgccgttat
    34321 ctacgatcac actgtccaca taaggaacat tttttatctt ttcacaatca taaacagggc
    34381 aaatgatttg ttgtttcaat acttgacctt tagcatattt cgataagtgt tgcatcacat
    34441 aataagttgg attcctccat gcttgtcctt tagcatctgt cagtattaaa ggtattgtgt
    34501 ttactaacaa tgattgacaa ctaatcttta cgcgatctgc gtgtcgtaaa atagccagcc
    34561 ctaatgaacc aaataataat gtatcttcca tagtaaagta acaccaatcg atgggactac
    34621 cgacttcaaa atccctatgt cttttttccg gttgggcatg tacattccat tcatcaaacg
    34681 ctagatacat tgttttctca cttctcaaat atccttttac ataatcacaa gtagcaacga
    34741 tttcttcaat ttgacgatcg aatcgataag cttttgcaag gtatgtcgct gtatcatcac
    34801 gctcattttt aggaggttta gtcaagtctt cttcatcata cttatcaata tagttatgca
    34861 aagctaaata atctacattc tcataagctt cttctaatgc gatacgatcc cattcagggt
    34921 acgatggtaa acgtggcgta gagcttccaa ctaagattgt ttcaattgaa tcatccacta
    34981 acttcatgac ttttgaggct tcatttgcta acctaccata ttcataagca ctcttagccc
    35041 ccacttgcca tggaccgtct agctcattcc ctaaacacca taatttaacg ccaaaaggct
    35101 ttttttgccc attttttatt cttaaatcac tccagaaggt tccttttgga aaattacaat
    35161 attctactaa attttttgct tcatcaattc cttttgttcc taaattcacc gtgaatatcg
    35221 gtttcgcttg tacatgactg atccatttaa aaaattcgtg gagtccaaaa gaatttggtt
    35281 ctatacctcg ccatgctaaa tcaatttttt ttggtcgttg ttctttccta cctacagtat
    35341 ccacccaatc ataaccagat gtaaagtttc ctccaggata gcgaatgaca cctaattcta
    35401 attcctttac ataagagagg acatcttgtc taaacccgtc accatcagct gtttcatggc
    35461 ccggttcata aatccctgta tagacgacac ttcccatatg ctcaacaaat gagccaaaca
    35521 gccgagaact gatctctcct accgtaaaat cctctctgat ttttactttc acttcttcgt
    35581 ccaacagctt tccctacctt tcatttccta tagtcaagat aatagataca agtttccata
    35641 cgccaccagc aaagataaaa ataaaaggag ggaaaagaaa tatgagcagt acaaatccta
    35701 ataacaatac gcaacaaaga atatttctca aaaaatcaaa aaatactttc catgcacttt
    35761 gttcaaaaat ttgtaaaaat gtgatatctt tgttttcaga aatcacatat atccaggata
    35821 aaagaaacat ggaataaata agtaaaacta catatccact aattgaaagt acgattgtct
    35881 gttcttctaa taattgatta acaagaaaat aaataatgat cgttaacaga ctaaggaaca
    35941 agttcttacc aaaatgttgc tttattcctt gaaagaaaaa ataaatgata tttcctgatc
    36001 cctcaccttt ccattgcgcc attacataaa aaacagcaca tgtagcaggc acgatagtaa
    36061 taacaggcaa acacacgatt acccagagaa tacctaataa gatgtaatca gaaaccacaa
    36121 tcaactgtcc ataaaattta gtctcaacta ttttcatctt cctcactctt tcattccaga
    36181 catagctgtt ccttgaacaa aatatttctg tgcaaaaata taaaccacta gtaatggcag
    36241 cattgaaata accgttccag ccattacagg tccataaaaa gaaatgttct gccctgtaaa
    36301 taaagctaac cctgctggta aagttctcat ttttgaacta cttgtcaata tcaatggata
    36361 aagcaagtca ttccaactat tcatcagcgt gaaaatccct aatgataaaa gagtaggttt
    36421 gctcaatggc aacatgattt tcaaaaaaat attaaactca cttaatccat caatgcgtgc
    36481 tgcctcttcc aaagaatctg gtaaagtaat aaaaaaggaa cgcatcatga agataccaaa
    36541 tgcagttgtc atacgaggaa tgatcaaacc tgcataagta tcaagcatgc caagcaaatt
    36601 gacttcaata aatagaggaa tcatgaagac ttggaatggg atcatcattg tcagcaaaac
    36661 gaaatagaac aaaactgatt ttcctttgaa acgcatacga gcaaatgcat accccgcaag
    36721 agaatcaaag aatacagaag tgatcactgt ccctccagca aaaatcactg tatttttgat
    36781 gtaatctaat aaagggatcg ttgtccaaac cttaatatag ttatctaggg tatagctatc
    36841 tgcaaatatt ctaggcggaa atgagataat gtctttttcg tgtttaaaag aagaaataat
    36901 cagccacaaa aaaggaaacg caataaccaa caatataact agcatactaa tcgttcgaat
    36961 gattttttta ccactaactg cttttcgact attcacagta actgtaatcg agctttctgg
    37021 tgccatacag gtttccctcc tatttcattt gtttttcttt ccgtgacatg tacacattag
    37081 tggagatagc tatagttgca acaatgataa acaaaaccga agacagtgct gatgcatagc
    37141 ccaattcata aggtgctgta aatcctcgac tgtaaatata ttgaacagct gtttctgtct
    37201 taaattgtgg gccaccagcg gttgcaacat atacttggtc aaacacttgc atcgaaccaa
    37261 tcagattcgt taaaatacaa aaacctaagg agggaacgat ctgaggtagc gtaatgttaa
    37321 aaaactgctg tcgatttgtt gcaccatcca tttgagcaga ttcatacaaa gagggagaga
    37381 ttccctgaat agcagtcaag agaatcgtca tagtaacacc aaaattcttc caaacagtca
    37441 tgatggctac cgtcggcata gcaagggttg gatcgcgaaa aaattgaggg atcggcaagc
    37501 cgacttgtga aaacaagtaa ggaatcgccc ctacattcgg atcaagcaac atagaaaaaa
    37561 taatcgcaat tgacgtcaat gaacaaataa ctggaatgta aaatgtcgaa cgacaaaaac
    37621 gattgaatct ggtattttta gataatgcta ctgctgctaa taaaccaacg ataatttgtg
    37681 ttggcgtttc gagtacggtg aaatataaag aatgaatcaa cgaattaact gctcgttcat
    37741 cactaaatat acgtgtgaaa ttttcaaaac ttacaaaatt tcttgatgta aagaaaatat
    37801 tcaaatctgt aaaactaata gcaaatgttc ctaaaagcgg tatgacagta aataataata
    37861 aaactaaaaa agatggagtt aaaaacaaat acgcagcacg atcaggacga ttccaatatt
    37921 tggtgaatcc gttcatatag tccacctcat ctttcatttt gataaaatat acaatcttgg
    37981 tttaaacgaa tctgaataag atatccgaac aatcgctgtg atttttgcaa taacacctat
    38041 ctgcaaatac taggtcgatc attcagatat ctgtatcttg ccaacagtct atcctgtata
    38101 aagttctgta ctatttttgc agtaattgat tgatggcatc atctgcatcg ttcatgagtg
    38161 tctcaggatc ttctcctgct aataattttt caatcagtgg attgatgata tcgttattga
    38221 tcgcagagat ttgggtaaaa cctggtaaat atggctctgc atagtccatt tgtttcgata
    38281 attcagatac aataggatca ttttttactt cttcatcttt tgctactgaa tccaagtagg
    38341 caggaaaacc attttcgata ctccattttt taccaatttc agttgtattc caatatttca
    38401 aaaactcata gacagcttct tttttattat tatctgtact tgccggaatg gcatatccta
    38461 cgccatctaa aatagctgtt tttttgtctg aatcactccc ttgtggtaag gtagtcacat
    38521 tataatcaat ctcgtttgct ttcaatccgt tatttaacca tggcccattg atttcaatgg
    38581 ctagttgacc agcattcatt aaattgtcca tttcggcgcc agaaatcgat tctggaccag
    38641 tcttgtctac atggatcatt ttttggatcg cttttaacgt tttaaggttc tcaggagacg
    38701 caaattcaga cttagtataa tcatcgttca ctaatttacc gccatttgct aacatccagt
    38761 tatataacgg tgcagttcca tccttattga agacaaaacc tgatacattt ttactactat
    38821 cagttaattt tggtgccatt tctgtcaatt cttcccaagt agtaggtggt tgttcagtat
    38881 ctaatccagc ttgtttaaac aaatcattat tccaatacat atacatgcct tgtacctgca
    38941 tggggacaaa atactgttta ccttctactt cacctaattc aatagccgtt tctttaaaat
    39001 cagatttatc tactccatca tattcccaga aatcgtctaa tggttgtacc gctccgtttt
    39061 ctacatactg tgcaaaatct ggataattta atgcaataaa gtcaggtgct tttttagaag
    39121 aaatcgctgt aggaagtttt tcattcaaat tggcccaagt catgatatcc atttcaattt
    39181 tgatgttttt atcatttgtt ttattaaatt cattaacgat ttcttgcaat atttcaccat
    39241 cagatgcagt aaacccattc caaaaagtca aagtcacttc tttatcagaa tcagattctg
    39301 ctgaacttcc gccaccgcaa gctcctaaaa tacctgttgc agctaccaac aaagagccaa
    39361 ttaaccattt tttacctttc attttttgtt cctccttcat ctgataaacg acatctgacc
    39421 acattccaag atagcggttt caataaacaa ttgattttcc catcttcagc ttttacattt
    39481 gttgaatgac ttggttttac ttgttcactt ttaggaagat tagttgcttt tgtatcaaat
    39541 ccactcattt cagaaaaatc aatcactccc tctaatgata attcagacaa gtgaacagtt
    39601 agattcattt cttcttctcc acggttaact gcaaatatga caagttcttt gtcatttatt
    39661 acggctatac tttctaaata cggcacttct tcaaaatctt ccgtcgcata gtttgctgaa
    39721 cgaatttgtg ggcttaacac ctctccttgt ccatacacag cagcttgcat aaatggataa
    39781 tagatcgtct gtctccaagc ttctccgttt gtttctgtcc tgattggtgc aataacgttt
    39841 accaattgag caagacaagc aatctttact ctatctgcat gtttcaataa agtgattaat
    39901 agacagccta atagtaatgc atcttcaaaa ttatagttat cttctagtaa aggaggagca
    39961 atctgccaag gcgctgcttg ctcatcttgt tccgctgaat gataccaaat attccattca
    40021 tcaaaggaca ggttgatctg cttcttacta tgtttcttcg ccttgactgt atcgcacatc
    40081 gctgtcactt cagaaatgaa ccgatccatg tctaaggaac gtgcaagata atgagaaaga
    40141 tcattttttt ggttgccata atattgatgc agagacaaat aatctatttg atcgtacgct
    40201 tgatcaagaa ctgttaactc ccaatcacca aatgtaggca tttgccgatt cgaacttccg
    40261 cataaaacca cctcaattga atcatctacg agtttcatcg ccttagccgt ttcctctgct
    40321 aaccgtccat attcttccgc tgttttatgt ccaatctgcc aaggaccgtc catttcattc
    40381 cctaaacacc atgtcttaat tccaaaaggg ctctcttgac cattcgaacg ccttaaatca
    40441 ctccaataag ttcctttagg aaaattacaa tattcgacta aattacgagc tgcatctact
    40501 ccacgagtac ccagattgac agccatattg atttcggccc ccacatgctg acaccagtta
    40561 taaaattcat gaatcccaac ttcattcgtt tcaattgtcc gccaagctaa atccaacctt
    40621 ctaggtctat ttgctatggg cccaatactg tcttcccaac gaaatcctga cacaaaattt
    40681 ccaccaggat atctaatcat gggaatgttc aattctttta ccaagtgaat gacgtcttgg
    40741 cgaaatccac ttttattagc tgatggatga tctggctgat aaatcccttc atatactgct
    40801 cgtcccatat gttcaataaa tgaactgtac aaccgcggat caactttgcc tacacgcttg
    40861 acactactaa tcaacattgt ggcttccata actgacctcc tttaatttat acatgaataa
    40921 tacacctcgt tgaaagcgtt gtcaataata taaatgttaa acttacttat tttttatcga
    40981 ttttaattaa ccaaaatatt aataatgatt aaaacaaata atttaatata acttttatta
    41041 acaaatatgt taatatataa gaaaaacctt ttaagaggtg atatatatgc aactacaaat
    41101 aaacgaagaa tccttaccgg tatatgaagc attagctagt aaaacaagga tcaagatcat
    41161 ccagctactt tccaaaaaaa agatgaatgt caaagatttg gcaaaagaac ttggagtgag
    41221 cagtgctatc acgacgatgc acgtgaaaaa gctagaggaa gcaaacatca tcaaaacaga
    41281 aaaagtagga caacaaaaaa tttcgagcct tcgtgtcgat aaaatcgata ttagttttcc
    41341 agaaaaaata ttcaatgctt ttgatacgaa agaaacatcg atacctattg gtcactatac
    41401 aaattatgca attgaaccaa catgcggttt agctacgatc catgatttta tcgggaaagt
    41461 ggatgaacca cgctatttta tggatcctcg aaggatggat gcccgcattc tctggtttac
    41521 cagcggattt gtcgagtatc aggcacctaa ttttttaaat acagaggata cattggagat
    41581 gttggaagtt tctgtggaaa tttcttctga atttcctttt tcaaatgaca attggccttc
    41641 agatatcact ttcagcctaa atggtgtgga gctaggaaca tggacgagcc ctggagattt
    41701 tgctgatatc cgcggtaaat atacgcctga ttggtatcca gataatctta atcaatacgg
    41761 cttgctgaag acgatccgaa ttaccaaaca cttgacgaat atgaatggag aacccttgtc
    41821 aaacatcacg atcaacgaca tccctaaaga gcaagacact tggcatttgc gaatcgaagt
    41881 gaaagacgat gccaaacatg tggggggctg tactttattt ggtaaaggat tcggtaatta
    41941 cgatcaggat atcaaattaa aagtttacta cagctgatat ttttcttttc tattttataa
    42001 aacaaaaaca gccaatccct atgaagggac ggctgttttt gctttattgt tatttccaga
    42061 aatcatccaa aacggaaatt ggcaaatgtc gtttatgttg tgtcttgttc caccatgctt
    42121 cgattttttc ttgtgcttga gcgggaactt cttttccttc tagataatca tcaatttcat
    42181 tatatgtgac acctagtgcg acttcatctg caatcattgg tttcccatct tcaagatctg
    42241 cagtcggaat ttttgtgtat aatttttccg gtgcattcaa tgcttgaagc aattgtttcc
    42301 cttgtcgttt gtccaaacgg aataatggca aaatgtcggc tccaccatca ccaaatttcg
    42361 tgaagaatcc agtaatgttt tctgcagcat gatctgtccc taatacggct cctgctcttt
    42421 ctccagctac ggcatattgc gtaatcatac gttgacgtgc tttgatattt cctttgttaa
    42481 aatcgctgat tcccacacct gcttcgctta atacttgtac ttgtgcatca acagctggtt
    42541 tgatatttac tcgcagacta acatcgggct ggataaagtc aagagcagct tttgcatctt
    42601 cttcatctgc ttgttcacca taaggaagac gaacagcgat aaattgatag ttttcatctt
    42661 ttgtttcttc acgcatttct tccatcgtta gttgcgccaa acggcctgct aaagtagaat
    42721 cttggccgcc gctgatacct agtacaaaag ttttaagaaa cggatatttt ttcaaatatg
    42781 ctttcataaa gtcaatactt ttacgtattt cttcttttgg atcaattgtt ggtttgacac
    42841 ctaattcatt gataatttct gtctgcaggc tcatttattc tcctcctatg cttctgtatg
    42901 aagttctgct acgctttttt tcattcgttc catgatagcc attttatggt tccagcattc
    42961 agtagacaga tcgactgggt atttttgcgg attcaagtct cgtttatact cttcccatag
    43021 tgaatctaga ttttcttttg tatattcttt gatttcgttt agtgcaggta attcgtaaac
    43081 tctttttcct tcgataaaga tatcttgtaa aactgggcga gctgtaaagt ctcgaacagt
    43141 tttattgata aacgtatgca ctggatggaa catgaagata gcttcttctt ctcgagggtc
    43201 ttcatcccat aatgtcacgt agtctccttc agatttccca tcaaaatttc gagtgatgcg
    43261 ccacacttgt tttttccctg gagtagttac tttttctgca ttgcttgata acttgatcgt
    43321 atccatcatt tttccttcat catcttcgat cgatactagc ttgaatactg caccaagggc
    43381 tggctgatca taggctgtga tcaattttgt cccaacaccc catacatcaa ttttagcttt
    43441 ttgcatcttt aaattcaaaa tcgttgcttc atccaagtca ttcgaagcat agatttttgc
    43501 ttccgtaaat ccggctgcat ctaattgttc ccgaacacgc ttagatatat aggccatatc
    43561 tccactgtcg atgcgtacgc cttggaagtt gattttgtca cctaattctt tcgccacgcg
    43621 aatcgcactt ggcacacctg atttcaacgt atcataagtg tcgactaaga acacgcagtc
    43681 tttatgggtt ttagcgtagg ccataaatgc ttcataatca tttccataag attggactaa
    43741 cgaatgagca tgggtcccgc tagctggtat cccaaatatc ttcccagcac gtacattgct
    43801 tgttgcatct gctccgccga tatatgccgc tctagttccc cagactgctg catccagttc
    43861 ttgtgcccgt ctagttccaa attccaatag tggatcttct ccgatgacag atttgatacg
    43921 agctgctttt gtcgcgatca acgtttggaa attgaccata ttcaacagtg ctgtttctac
    43981 taattggcag tgcgctaaag gcccttccac ttggatcaat ggttcatttg caaaaactag
    44041 ttctccttca cgagcagaac gaactgttgc tttaaattca aaattttgta aatactccaa
    44101 aaaaccttca ggatagactt ctaatccacg caagtaagca atatcggaat cattgaacgt
    44161 cagattctct aaataattga ctaaccgttc caatcctgca aaaaccgcgt atccgtggtt
    44221 aaaaggcatt tctcgaaagt aacattcaaa aacagcatgt aaatcagctc gacctaattc
    44281 ccagtatgtc tgcatcatat tgatttgata tagatccgta tgcagcgtca aactgtcgtc
    44341 tgggtaagta ttattcatct tgtattactc ctcttggcaa gttttcattt cctacccatt
    44401 ataacaaaaa aaattaatag gaaaagaaat cttccatttt ttctgaaaaa gcacatgttt
    44461 tacggatatt tcttgctaaa aatgaactcc atctgctaaa actatccttt cttattggta
    44521 tttaacctaa acgagaaaga atacttattt tattcaatgt atccgagaca cgaagaaatt
    44581 agcgttttct atttttctta cttgataaaa taagtagatt ttcctactac aatcgtagcc
    44641 gttaagatac cgatgattga acaaatataa ccaaatgtct ctacaacata atcaatggca
    44701 aaagtttttg aagcaataat acgtctttcc ttttttgctt cttatcccca aagaaacgga
    44761 tataacttat tatgtattga atttttttgg aaaggcgaat tatgacaaga tataccttgc
    44821 gttgaaaaaa ctcgcggaaa aaatatcaca cgtttttaaa aagatacaaa gtaagaaagc
    44881 agctgtcaat agaatggaaa caaaaatggc gagacatcaa tcagtctcgc cttgttttt
//
LOCUS       DARBET010000017        41327 bp    DNA     linear   BCT 07-NOV-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4027
            SAMEA114569841-rid20112183.denovo.017, whole genome shotgun
            sequence.
ACCESSION   DARBET010000017 DARBET010000000
VERSION     DARBET010000017.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA114569841
            Sequence Read Archive: ERR12203666
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 41327)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 41327)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001972618.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001972618.1
            Genome Coverage       :: 132x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/07/2023 13:18:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,041
            CDSs (total)                      :: 2,975
            Genes (coding)                    :: 2,908
            CDSs (with protein)               :: 2,908
            Genes (RNA)                       :: 66
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 67
            CDSs (without protein)            :: 67
            Pseudo Genes (ambiguous residues) :: 0 of 67
            Pseudo Genes (frameshifted)       :: 26 of 67
            Pseudo Genes (incomplete)         :: 28 of 67
            Pseudo Genes (internal stop)      :: 20 of 67
            Pseudo Genes (multiple problems)  :: 6 of 67
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..41327
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA114569841-rid20112183.denovo.017"
                     /isolate="VRE4027"
                     /db_xref="taxon:1352"
                     /country="Denmark"
     gene            128..1555
                     /locus_tag="SCJ86_001007"
     CDS             128..1555
                     /locus_tag="SCJ86_001007"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339527.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HEG1168588.1"
                     /translation="MIEEYIEKNILRQLFLCGQFYVNKEVNLEKLSNLLHVCKTTLLN
                     DINNIKKEFEEQIAYTHREKDRYTLYFSEHIPRCKIMQQLSQNSLFLKTCLLYLEEDE
                     PDYLQLTECEFISVSKAYSLKKQVLAYFNDCGIEIDRYSPRFTEMERRLLLLNVSYRL
                     GGFNSWELPESFFERADRFIESVTENSGRFYDKENKEILSIGFAISFLRQQVCAVTID
                     SKFIEEIKKRPIYNYVESAWENTDFQTYYKKEEFAFILTLFNLCNYGFHSYQLIAEDF
                     QQLHQVFIDNTPEIKELVATFESHFNQELFGNQPFERALIHLMRSAWDNYQLFMPEKF
                     YLLNEEQTNLYKEVQTIFSSWSSQLPYDLRLNPNCMRAFVIELSGILRLTKEHLTIYI
                     VTNSDVHYLIYREALEAVTTFDFQVAPTIYSSISDIKKYAQQSSNRVLCERTLYTPDA
                     VQYENIIPISINTIDRAIISAVQNK"
     gene            complement(1647..2012)
                     /locus_tag="SCJ86_001008"
     CDS             complement(1647..2012)
                     /locus_tag="SCJ86_001008"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1622 domain-containing protein"
                     /protein_id="HEG1168589.1"
                     /translation="MTTELMQFLLPYFEKLVLGLDIFAICLLVWGVCLSIKDFLKYLF
                     VHTKVQSDRVSENNAIKKMLGGYILLSLEILVSAGIIESIIKPTLQDIFQLAALVVIR
                     TIISYFLNKEIGPLDVQNK"
     gene            complement(2009..2374)
                     /locus_tag="SCJ86_001009"
     CDS             complement(2009..2374)
                     /locus_tag="SCJ86_001009"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337917.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1622 domain-containing protein"
                     /protein_id="HEG1168590.1"
                     /translation="MFRSNLIEFLEWIIAILNICSIGILIVGIVLVLFKLFDWSHVKK
                     NYAERNTRNADTRKLLASYILLSLEVLVVADIIESVIKPTWTDILKLALIIIIRTVIS
                     YFLNQEIEDKEKQRRGEVG"
     gene            complement(2566..2814)
                     /locus_tag="SCJ86_001010"
     CDS             complement(2566..2814)
                     /locus_tag="SCJ86_001010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEG1168591.1"
                     /translation="MKNASLFSLSISKDSSFLLECQMIGREEKGNFDKKEEVSDFPVN
                     KNRVGKKKRFEEVYGSIFRNLGTKKAPRQCFFEKLMTD"
     gene            2813..2941
                     /locus_tag="SCJ86_001011"
     CDS             2813..2941
                     /locus_tag="SCJ86_001011"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019724379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methanol dehydrogenase"
                     /protein_id="HEG1168592.1"
                     /translation="MSKKLIKVGIGLGLLALGAAYLGKKTGLFEDDSHLYDEFESI"
     gene            2998..4692
                     /locus_tag="SCJ86_001012"
     CDS             2998..4692
                     /locus_tag="SCJ86_001012"
                     /EC_number="4.2.1.53"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344632.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oleate hydratase"
                     /protein_id="HEG1168593.1"
                     /translation="MRKSKAIMIGAGIANMAAAVYLIQEGKWKGDQITFYSLDDHGSN
                     DGAPTDTVVDEYWNKNHPLENQKGYIARGGRMLNYRTYVDLMDLLSRIPSVTEPGMTA
                     EEDTRDFDAKHRTFDKARLMEGGKGIIDGGKLGFNNKDRILLTKLIAMPDSEEEKLDN
                     ITIEEYFKDDPHFFETNFWFMWETTFAFRTRSSAQELRRYMHQMIYEFTQIEHLVGVN
                     RTRYNQYESIMLPLIEYLKEQGCKIILNRRVTDWEFKETPMQDEITVTGLKITNTLTQ
                     EEEYVVVDEDTAVIFTNGSITDSATLGDYETPAVENMDYGAASSLWKKASERFYNLGN
                     PDKFFADRDASEWVSFTLTTKNHLLLNEITRITTQEPGNALNSFISTTPITPLGQKDV
                     NMSIVVHHQPHFTSQKPNETVIWGYFLYPRRRGEFVDKQYIKMNGKEMLEELIGQLSK
                     VDPGPVNIREKETEIFDSVINNIPVYMPYASALFNNRAKSDRPKVIPKHSTNLAFTGE
                     FVEQPYQMIFTEQSAVRSGEIAAFHFAGIPMSRLVKTPRYDKDIPTLARAAKKMFE"
     gene            4696..4815
                     /locus_tag="SCJ86_001013"
                     /pseudo
     CDS             4696..4815
                     /locus_tag="SCJ86_001013"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349726.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     transferase subunit TsaD"
     gene            5343..6476
                     /locus_tag="SCJ86_001014"
     CDS             5343..6476
                     /locus_tag="SCJ86_001014"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736480.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alcohol dehydrogenase catalytic
                     domain-containing protein"
                     /protein_id="HEG1168594.1"
                     /translation="MKAVTWQGKQKMEIRNVDDPKILEPTDAIIRITATAICGSDLHL
                     YHHGEAVLEKGYVVGHEPMGIVEEVGSQVQTLKKGQRVVIPFNISCGHCHFCLNHMES
                     QCDESNPDQLYGGLFGFGKLNGNHWGGQAEYLRVPYADSTSFVVPDNDLPDEKVLFLS
                     DILPTAYWSVENAGVKKGDTVVILGSGPVGLFAQKFAVMAGAERVIAVDPVQHRLDKA
                     ANYNGVETYFLEDTAQAGTELYELAKGGADVIIDCVGMDGLEPVKEKAKNLVSLQSGT
                     ISPLQMASQAVKKFGTVQVTGVYMTPASSYPLQEFFMRDIAVKHGQAPIIHLMPKIYA
                     MIAEGLFDPTQIITHSLPLSEAAQAYEIFDKKEDKNIKVVLKP"
     gene            6507..6827
                     /locus_tag="SCJ86_001015"
     CDS             6507..6827
                     /locus_tag="SCJ86_001015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629521.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YrhK family protein"
                     /protein_id="HEG1168595.1"
                     /translation="MPKIKRNTHEIEIGKEEDIEIRGRRFRFYFQNRYTLISLAVDLL
                     TGIFYIFGSLASLTKIPDRYGTYFYLAGAIFLTIRPILRIVRNIFIYDEKKAMKRTSK
                     KKEA"
     gene            complement(6856..7320)
                     /locus_tag="SCJ86_001016"
     CDS             complement(6856..7320)
                     /locus_tag="SCJ86_001016"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331957.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEG1168596.1"
                     /translation="MVTYNWIKLREHPELVKQSANWFSSKWGVPAVVYEESIRTGIAN
                     KESIPQWYVVLDEKETIVGGAGVIENDFHNRPDLTPNLCALFVEPEKRKQGIAKRILA
                     EIRKEMHQLGYEKLYLITDHTTFYERYEWTFLTMVKEESGTTARLYQSDTIG"
     gene            complement(7452..8705)
                     /locus_tag="SCJ86_001017"
     CDS             complement(7452..8705)
                     /locus_tag="SCJ86_001017"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HEG1168597.1"
                     /translation="MDKFVAFMEKHFIPVASKIGAQRHLVAIRDSFMVSMPLMILGAL
                     AVMINNLPIPGFQELMNSIFGGESWKGFGAAAWNGTFAILSVLIAFLLAYHLANGYRK
                     DGVSAGVISLGSFFALGGALGMSSNGLFIAIIVGIISTEIFVRLSGNEKLIIKMPDGV
                     PPAVAKAFASLLPAMITISAFALVAAIFAAFGVDDIVGSFYTVVQEPFMGLANSYPSA
                     LLLAFITPFLWFFGLHGANMVDPLMQTINAPAIEANVNAIAAGHSAPFIVNKPFFDSF
                     VNLGGTGATLGLLLAIYLVGRKNKPYMVVTNLSIAPGVFNINEPTMFGLPIVLNPIMF
                     IPFILTPMVLVSVAYFATSTGLVPAATVMPPWVTPPIIGGVLATKSIAGGVLAAVNLV
                     LSILIYLPFVKVATIQERRKEALDA"
     gene            complement(8734..9051)
                     /locus_tag="SCJ86_001018"
     CDS             complement(8734..9051)
                     /locus_tag="SCJ86_001018"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005877906.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS lactose/cellobiose transporter subunit IIA"
                     /protein_id="HEG1168598.1"
                     /translation="MEDQKNLEAIMGLIMFGGNAKSDAMEAIAAAKKGDFTLADEKIK
                     DAEESLVQAHHSQTEMLTQEAQGNHVEVTLLTVHSQDHLMTSIAFTDLAKEIINLYRR
                     LNA"
     gene            complement(9077..9397)
                     /locus_tag="SCJ86_001019"
     CDS             complement(9077..9397)
                     /locus_tag="SCJ86_001019"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292494.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HEG1168599.1"
                     /translation="MEKKTIMLVCSAGMSTSLLVTKMQKAAEAKGMEADIFAVSASDA
                     DNQLESKNVDVLLLGPQVRFMQNQFAEKLAPKGIPLDVINMADYGMMNGEKVLDQAEK
                     LINK"
     gene            complement(9861..11276)
                     /locus_tag="SCJ86_001020"
     CDS             complement(9861..11276)
                     /locus_tag="SCJ86_001020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331958.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 1 protein"
                     /protein_id="HEG1168600.1"
                     /translation="MKITIPKNFILGAASSAWQTEGWKGKKEGQDSFIDSWYKNEHFV
                     WHNGYGPAVATNFMEQYQQDVDLMKSIHLTHYRTSINWARFFTDYENCIVDEEYAQHI
                     SEMIDSLIEADVEPMICLEHYEVPAFLLEKYDGWSSRKVVDFYAKYAQIAFDRYGDRV
                     KNWFTFNEPIVPQTRCYLDAVRWPHEQNTKKWMMWNYHKVLATAKAVEIYRSGNYGGR
                     IGCILNPEMVYARSNSPEDQKAAYMYDLFYNRVFFDPMVKGYYTEEFLELCQKQSIYF
                     DPATEDLETIQHHTVDFLGINQYYPKRVRAPRYQWNEETPFHPERYYEVFDLPGKKMN
                     NSRGWEIYPKIMYDIAMYLKENYHNIPWLITENGMGREYEEQYMDEKGIVQDDYRIEF
                     IGQHLHYLIKATEEGANCEGYMLWAFTDCVSPMNAFKNRYGLVRIELNDERSRTLKKS
                     ASWYQTLIDKRELDLPDEIYK"
     gene            complement(11293..12609)
                     /locus_tag="SCJ86_001021"
     CDS             complement(11293..12609)
                     /locus_tag="SCJ86_001021"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HEG1168601.1"
                     /translation="MRGSGMSFMDKLANVLGNVATRINNLRYIMVIKNAFAALIPVII
                     TGAFGTLFSAMVFDSENGLAQISFLRFLEELKPIASAISYVTLSFLTIYAVFLIGIEL
                     AKLNKVDGVFPGIIAVMSYLSVNPTVYELVNENQTVIVENVLAKQYTDTKGLFLGMLV
                     SILSIELYCWLRKQKKLQLRMPDTVPTNVSASFSALFPTILTVSGIAAAGFIIKELTG
                     MYLYDIIYNVVQRPLEGVVQGLPGILLLMFIAQVFWVIGIHGNQMVKPIREPLLLAAI
                     AVNTEAFEAGKEVPNIITMPFWDMYMSMGGSGVTIGLLIAVLLVGKREDMKQITKLSL
                     APGIFNINEPVIFGMPIMLNPILAIPFILTPLITGTIGYFATALGFAAKAVVMVPWPM
                     PPIVNAYLATAGNIGAVITQLVCIIVSILIYLPFVKVANRVENDGI"
     gene            complement(12759..13457)
                     /locus_tag="SCJ86_001022"
     CDS             complement(12759..13457)
                     /locus_tag="SCJ86_001022"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292490.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FadR family transcriptional regulator"
                     /protein_id="HEG1168602.1"
                     /translation="MEENKTLVEVTTDKILRYLKEMQLQVGDKLPNEYELSDYLEVGR
                     STVREAVRALASRNILEVRQGAGTFVSDKRGIANDPLGFSLIEDQDRMIRDLFELRYL
                     LEPKMAGLAAEYATKNQIVQMKKLTQKIETSFREGTQEHVQLDIDFHTMIAEASGNVA
                     LLHIIPIINESIALFNKNYDIQHLKIETIDIHTEIVQAIERRNALAAFDAMTVHMAIN
                     RRELQKIIGEKETN"
     gene            complement(13746..14702)
                     /locus_tag="SCJ86_001023"
     CDS             complement(13746..14702)
                     /locus_tag="SCJ86_001023"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321245.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HEG1168603.1"
                     /translation="MEYRKSGHSGLKLPILSLGLWQNFGDYDPIHNQREILRGAFDMG
                     ITHFDLANNYGGPAGAAEKNFGRIFREDFQAYRDELIISSKAGYHMWEGPYGEWGSRK
                     SIISSCDQSLQRMGLDYVDIFYHHRPDPDTPLEETAEALMQLVRQGKALYIGISNYNG
                     EDTKKMTEILKRKEAPFIIHQMRYNMFSRALLEDDLSPVLEEEGLGAITFSPLAQGLL
                     TNRYLHGIPEDSRAHRKEIPFLSEEQVGSTLEKIKALQTIAVSRGQSLAQMALAWNLR
                     QKSVTSVLVGASRLSQLQESVRMMDNLDFSPEEELRIDQILE"
     gene            complement(14815..15648)
                     /gene="msrA"
                     /locus_tag="SCJ86_001024"
     CDS             complement(14815..15648)
                     /gene="msrA"
                     /locus_tag="SCJ86_001024"
                     /EC_number="1.8.4.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631744.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide-methionine (S)-S-oxide reductase MsrA"
                     /protein_id="HEG1168604.1"
                     /translation="MDEEQIFIQTLYNLILNPNTRDWERKVLIQTKNDIRENISVKEQ
                     LSKLEATLRPLAIRMNLTPDVMDFYLLITEGFDKEQKYDFSKHAMQDADYQERAVFAG
                     GCFWCMVEPFESKKGILSVLSGYTGGHVEKPNYDQVSGGYTGHVEAVEIIYDTREISY
                     SELLTIYWQITDPTDTFGQFQDRGKQYRPVIFYQVERQKELAEQSKQKLDSSGTFHQP
                     IVTKIEPAGTFWPAENYHQQFYKKQPKRYKKIQQARNQFLIYQRVKNKWQKNIRKNHF
                     D"
     gene            complement(15823..17457)
                     /locus_tag="SCJ86_001025"
     CDS             complement(15823..17457)
                     /locus_tag="SCJ86_001025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353756.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEG1168605.1"
                     /translation="MKINKKVVILSGLIIVSSIYSFIFFKNTQSIYTTGDLSFHLSRI
                     KGLSTIFTSPINYETFNYTGYGVNYFYPFLTFFPAVMLYWMTKNLIVSYIIYVWLLNL
                     CTTLVAYHYGERFLKQKKAAFLFSCLYIFSAYRTVDIYYRSAIAEAIAITLVIPVLFY
                     AYQIISGKEEKYPSVKLALSMSLLVYSHVLSTLMSTALIIIFIFIRLVSKGFKNADFI
                     AIFKKLFSAAGMTLVLTSAFWYPMFEQMLYQKINKPSVTNLYAHASNVFDSLTEAMNN
                     DLTTYSMGLVGLLSLCIPLILFKKLTNIEKKIYYGTCLTWLATTSLVPWYLLQNTPAK
                     LLQFPWRILSLQIIFSSLILTMIFFKNRRYNKTRELFYLFMTVLLLITLTVSSKKNFN
                     QKIISQPGHIVVNKETVEAYTTKGMDYFLYDYSPIASLENKEHIIKHEAKIGSQWVNL
                     PYKTTGSSIIYRVTNEKSQVITLPVYAYLGTNVEINHQPVKEQVNANGLVQISGVKGV
                     ADIKVSTSYTVTTIISYLVTLTAYLCLFFKIVFHKR"
     gene            complement(17444..19312)
                     /locus_tag="SCJ86_001026"
     CDS             complement(17444..19312)
                     /locus_tag="SCJ86_001026"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002367894.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEG1168606.1"
                     /translation="MISIIWFLFFFIFFSYLKVIKIDSFLVYHTSAMKNLTQNLLKKD
                     NSSILKIELKKENNKSMKISKKAFLLVVLILLSTLYSVNFMRNAQEIYTTGDLSFHLS
                     RIKGLSSIFEGPINYTTFNNYGDGLNYFYPFLTIIPAVVFYGISNNLILSYVLYIWLL
                     NICTILISFYYGEKFFKKIDAAFLFSCLYTFATYRTVDIYYRSAIAEAIALTIIPAVL
                     YYAYGLIFNREKKVIALSVSISLLVYTHVLTTLLCSALIACMIIVRLFLSKQIKNDIK
                     FVVVNLVKAVFLTCALTSYFWYPMLQQMNYQEIHSPFKTDLQLEALSISDSIIQALNN
                     DITTYTMGLLGLVALIFPLFILKEMEKKEKIIYVGVVSTWVCVTNLFPWFLFQKTPIS
                     LLQFPWRILGIQIFFSSMIITLVFMKKTMNKKYSWLIIGATVVFLLVTSIAAKENYAK
                     VIQSKHGHIVINEETLPRYTTSDMGGLYDYAPTEAIKERPHLEKHEVKVGESWEKGKY
                     KAADNNITYTVASNKKQKVTVPVYAYLGTQVKVNGKVVNDSYKKHGLVNVDVNAGENK
                     IEITTKYTPTALMALWISILTYCLVVYSSFKRFAPNKLKDSRYVTKKEINKKNENK"
     gene            19278..20915
                     /locus_tag="SCJ86_001027"
     CDS             19278..20915
                     /locus_tag="SCJ86_001027"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002356834.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEG1168607.1"
                     /translation="MKKNKNQIIDIIFMLFLSFAYVIPLFMSKGIYHSVNQDTYFHLS
                     RIIGLDNVWSSPVNFNNFDHHGTMMNIFYPWLTLYPAFLFYKMMGNLVLSYNIYYFFI
                     TFLTMVVSYFSMKQIKNNRYISLLFSIIYTFSAYRAIDIFHRASLGEAVALTFLPLIL
                     MGCYEIYIRDYQKWYWLSIGMTLVVYTHLLSVAMVSVFIGGTLFLSFYFWDQKIARLL
                     SLLKATVLTFFLSAGFLIPFIQQSRAQELKVPLGKELSGMAPSDMLTHILNNNYNNYT
                     IGLFLFLGLIGAFIFIKKLTADDLFIFFLGVFVLFCSTNLFPWQLFNHTPVKSLQFVW
                     RLNGFSSLFIAYTMSIVIYYIFSTKNNSWKIMVFTFLALILHLSGVSNSIHANKDSLT
                     LIAPEDAVAIAENYNHTDYANKESIYHPDMINNDQYLLGNQEINPTTHFMSNKLTIEL
                     DNSNNKNTVLTTPIYRYKGQVASINGKLVQTKLSKFGTTELTIPPGINKVVITYQYTK
                     LAIASRYLSIVTLILFLLYRFTFSKQKQPRREVQHSH"
     gene            complement(21060..21800)
                     /gene="truA"
                     /locus_tag="SCJ86_001028"
     CDS             complement(21060..21800)
                     /gene="truA"
                     /locus_tag="SCJ86_001028"
                     /EC_number="5.4.99.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292328.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA pseudouridine(38-40) synthase TruA"
                     /protein_id="HEG1168608.1"
                     /translation="MRNIKLTIEYDGKRYSGWQRLGDDDKTIQGKIEKVLHQMTNEEI
                     EIIGSGRTDAGTHARGQVANFKTNTEIGLAEMIDFMNRYLPRDIVIKRIEEMPERFHS
                     RYNAVGKKYSYYVWNNVIPSAFERNHSFYFPQELDMDKLNAACEKLVGTHDFIGFSAL
                     KKSKKSTVRTIDELSIERKGEMLHFTFVGDGFLYKMVRIIMGTLLEIGTGAMPIETID
                     EIFESKVRQHAGETVPAQGLFLDEVYYR"
     gene            complement(21991..22626)
                     /locus_tag="SCJ86_001029"
     CDS             complement(21991..22626)
                     /locus_tag="SCJ86_001029"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292320.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF998 domain-containing protein"
                     /protein_id="HEG1168609.1"
                     /translation="MDFLRKFGFFFLLLGVLSDFLTPYVLGLYYPELDQMKSVISVFG
                     EVSSPVRKAFLIWSVVSGIFFILSLPALYAAFVSTSRVLATLVTAAIAFYGIGDCIFT
                     GLFSINTHESSWNVSTWVHNIGSGLGYSGFLLFPVLLFLIYRKNGQMDVSHLYLGLSV
                     ISLLFAAIYGLARIPSINSWPLLNKIGFCQRISFFFNYVPIARLSILHLKQ"
     gene            complement(22773..24014)
                     /gene="sstT"
                     /locus_tag="SCJ86_001030"
     CDS             complement(22773..24014)
                     /gene="sstT"
                     /locus_tag="SCJ86_001030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338586.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine/threonine transporter SstT"
                     /protein_id="HEG1168610.1"
                     /translation="MLEKFLDMSLVQRIVVGILVGILFGIFFPTWTFISILGTLFVSC
                     LKAIAPILVFFLTMASIAKHKIGNKTFVKPILILYLVGTLLSALVAVIVSSIFTVPIT
                     LQETVSEKAPQSLESVLSTMLTNVTQNPVQSLIDANYLGVLFWAVLLGLAFRARSEST
                     KELVDQISIALSQVVQVIIAFAPIGILGLVYQSIATTGIAGLAEYLQLLLVLIGTMLF
                     VALVVYPFLTFLFIKENPYPLIFFCLKESALPAFFTRSSAANIPINMMLAERLKLTKE
                     SYSISIPLGATINMGGAAITISLMTLTAVHSLGIEAPFVLKVLLCILSALAACGASGV
                     AGGSLLLIPLACSLFGISNDVAMQIVGIGFIIGVIQDSMETALNSSSDLLFTAIGELG
                     ARKRSGEKIKLKDCLDGLSER"
     gene            24212..25210
                     /locus_tag="SCJ86_001031"
     CDS             24212..25210
                     /locus_tag="SCJ86_001031"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEG1168611.1"
                     /translation="MFLSNFFVRCLFLKKIKKESYGFLTIFVACIMMVLQRILISAVT
                     IPTYATNPGRFYIYTFLQTSVVIGANLIPLYMGYQYQKIARLKVLHYLSRFAFIFLIA
                     TLVCNIFFYVQAGSLNIRDYWLILTPISQNYFTYAVSCTLLFCSIPYTVGFLNKLSKT
                     TIKYLLLFLTIGLVGCSTLFNKDPFALQNGQSILWIFYLFLMGYGLKEWNWKEKVRYK
                     LPQVFISLIVLFGLIILMTQISLIIRGNTDTALRFSKPYSLFAMYYTFSSFLFLEMLS
                     DKLGIKGRGNFLAVFLIVTQVICNFPLVSYRVSTFMKREFPNSGKAWLINIGQFFI"
     gene            25223..27241
                     /locus_tag="SCJ86_001032"
     CDS             25223..27241
                     /locus_tag="SCJ86_001032"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302655.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfatase-like hydrolase/transferase"
                     /protein_id="HEG1168612.1"
                     /translation="MSLLVIFLFYIQKIRFIHRFIEYFAFDSIEQLTQRWKKRIHWLF
                     VHKSYFLVAAFFYCFTFVQIFVLEPKEGWKETIQVALKIFTQRQAPMILTIIIIMGFF
                     FLLLLISNRFWYALTATLIINLLLTISTVIKMEMREEPVFPSDLKMLTGLSELLSMVS
                     PVLLIVGGLILLLLLITSIIVQRRLQKQYSLKIHWKRRFISIAVLLGCFSGVFFINHK
                     NSPSFLLFNLFKVNKTFFNQQGAVKDNGPVIQFLNNIDIKIMEEPAGYSKEKINGIMK
                     KYNTKADEINQTRSDWLNNQTIVLNLSESFSDPARVPNLTVPTDPIPYIKSIITEGTG
                     GLMLSVGYGGGTANMEWEGLTGLSISNLSASLVTPYTQLVERQKISPNITDLFDEKIA
                     IHPFTAALYRRKQVFDKFGFDRFYYLDSPDKLTYTDKIEQSPRISDDSAYKETLKALQ
                     SNTETSQFIQLSTMQNHMPYDDYYSELNYTAEGTAVIPNRRKELQTYMQGLHYTDTAV
                     KKFIQEIDKIDKPITFVFYGDHLPAIYSGNDSKKYGLEQHQTDYFIYNNKYSREQAAT
                     LNKEVVAPNNFGAMALEHANIKVTPYYALLTEVSNHLPALTIDPTESLSNRFNGKQVF
                     VTEDSQIIQEKNLTEKQKEILEDYRLIQYDLVAGNQYSATWATQKIKD"
     gene            complement(27301..28017)
                     /locus_tag="SCJ86_001033"
     CDS             complement(27301..28017)
                     /locus_tag="SCJ86_001033"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299292.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4767 domain-containing protein"
                     /protein_id="HEG1168613.1"
                     /translation="MKKRILVTIGLAFLLAGCTNGNESKKESTKETAKATVATTTTTS
                     TESKSTAASTETADTSTSSESSKTTETTQTSTSSSSVEASSSSSESSEQVEQTLWNAD
                     KSAQLEAFMTKWGQTMGQQYKSYTNQMSVDLYGLKVPQAILNGEWKMAIGGVPVSAEW
                     SESGTGQADYQITAVYSDAETEPYLKKHVYLFGFQQNQPKVLVTQQNQGNPDNYLYFN
                     ETANNELKNGFNQIVYGQAN"
     gene            complement(28244..28786)
                     /locus_tag="SCJ86_001034"
     CDS             complement(28244..28786)
                     /locus_tag="SCJ86_001034"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375152.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein"
                     /protein_id="HEG1168614.1"
                     /translation="MNIVEEKFVQETKQADIDHHKPTAGAMVGHVLANFKLEEMKLIQ
                     AQLYVLGTSSQELSTLFSEYADQEARWFNKISHELVIENEIVPSTLEEVMRYGKIYEH
                     GQNKYLPASTMLENFAKDFDFQNVFITRAIKLAEKEEKYGLQQLLVELLSFNKEIIYQ
                     VQRLQGKTVRQDLDEEDDDD"
     gene            complement(28791..29018)
                     /locus_tag="SCJ86_001035"
     CDS             complement(28791..29018)
                     /locus_tag="SCJ86_001035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation transporter"
                     /protein_id="HEG1168615.1"
                     /translation="MKKIIMQLDTLACPSCMQKIEHAVSQQPGVSNTKVLFNASKIKA
                     NFDPDEVHPEQLAAVVEKLGYEVQSAKVKEA"
     gene            complement(29097..30977)
                     /locus_tag="SCJ86_001036"
     CDS             complement(29097..30977)
                     /locus_tag="SCJ86_001036"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328704.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heavy metal translocating P-type ATPase"
                     /protein_id="HEG1168616.1"
                     /translation="MKLQKWIQEHRNHITALSAVLIIFGFIGKFTGFVLFYNIALAIA
                     SIIAVIPIMIHAYQAVKVKVISIDLLVTIAVLGAFMIGEYKESAIVTFLFLFGSYLEQ
                     KTLTKTRESIKALTEMAPSTASVVQEDGTTEEVEIDDVEEGDIVLVKTGGAIPVDGRV
                     IDGKGYADEAAVTGESRQVVKISGDEVFSGTILADGYVTIEATKVGDDTTFAKIIELV
                     EEAQDTKSHAEKFIDRFSQYYTPTVLLIALVVGLISRDFRLAITVLVLGCPGALVIGA
                     PVSNVAGIGNGAKRGILVKGGEAMDTFSKIDTFVFDKTGTLTKGTTAVSTIKNYTEDE
                     GDSLALVARLETLSDHPLGRAVIHYAQEKDIDFQQLAVKDNQTIKGQGITAEIDGHTV
                     CAGNTKLIAAHKLSLTPKQVQDLHQLQQTGSSVIIVAIDKKITQIIGVSDVIRPEVAE
                     QLAKLKQAGAKQLVMLTGDNQMTADYVADMLGIDEVHAELLPDEKVQFVKKYQEQGLR
                     VAFIGDGINDSPSLAAADIGIAMGSGTDVAIETSDVVLMQSNFESLVHAYRLAKKTVL
                     NTRENVVIAIGVVLFLLNGLFAGFIYMASGMFVHEASILIVIFNAMRLLYFGHESKEE
                     KS"
     gene            complement(31166..31780)
                     /locus_tag="SCJ86_001037"
     CDS             complement(31166..31780)
                     /locus_tag="SCJ86_001037"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333990.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEG1168617.1"
                     /translation="MKLTFKPLEKKDYKKAIYFAVEGMNFERYMENELLLNLYGRYFL
                     YMELNRATQILALYAEDELAGVLLAEIKGEKKKHRSFGQQIYVKIFDFLQNIFAKESK
                     GAYETANEQMLAAYLKKHSPDGEIIFLAANPDLKTKGIGTKLLTEFEKREKGKEIFLF
                     TDSGCTYQFYERRGFERLKEKISKIKILDKEIDLRCMLYRKIIQ"
     gene            31922..32482
                     /locus_tag="SCJ86_001038"
     CDS             31922..32482
                     /locus_tag="SCJ86_001038"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377045.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HEG1168618.1"
                     /translation="MKKTDRRVKKTEKALAETLSTLLVNKKIQAITIRELTETADVHR
                     STFYTHYKDIYDLYDQLESNFFRDLNEILSYDPAHSYEELYTRLIDYVYANTFLYRLF
                     ILNHGDISFQKKVKQVIEENYLNIWLYEDKKTIITEEMRFFTTYHVQGCLSVLNRWVE
                     SNYKYSKKELLELLQKLNDHIEKIMP"
     gene            complement(32787..33101)
                     /locus_tag="SCJ86_001039"
                     /pseudo
     CDS             complement(32787..33101)
                     /locus_tag="SCJ86_001039"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334404.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="type I restriction endonuclease subunit R"
     gene            complement(33355..34990)
                     /locus_tag="SCJ86_001040"
                     /pseudo
     CDS             complement(33355..34990)
                     /locus_tag="SCJ86_001040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294751.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(35545..36108)
                     /locus_tag="SCJ86_001041"
     CDS             complement(35545..36108)
                     /locus_tag="SCJ86_001041"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294754.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="restriction endonuclease subunit S"
                     /protein_id="HEG1168619.1"
                     /translation="MITVVHKERQEKSSSDVRLWVYLGSISTKIQYGYTDSAKKQGNV
                     KFLRITDIQEGRVNWSSVPYCDISNSKLVDLRLEENDILIARTGGTMGKSFLVKEISE
                     ESVFASYLIRIRLVEKLLSEYVDCFLDSPLYWKLLEKISYGTGQPNVNGTNLSKLLIP
                     LPPLEEQQRMTTKIEMIRRSIRRINFE"
     gene            complement(36123..37166)
                     /locus_tag="SCJ86_001042"
     CDS             complement(36123..37166)
                     /locus_tag="SCJ86_001042"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330003.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEG1168620.1"
                     /translation="MTQRVAIMILVLLVIGLLVYYVLKFKHWKQQRIHQDIEKKLKRY
                     PIVQAAWEKAEAKQYNIPGLTETRMVVPETGENEVCQWMTPQGLAFSQDFVFISAYCY
                     DHQHHSIIHVLDRETGQPIKLLILPKRPHVGGLVYDTKRELLWLTITGSATGRVAALR
                     LIDILADTSEETGQPIAYWLTTDLSEIPQASYLTQNNDQLVSGNFTLKGEGQLTFYLL
                     PTIAEMKTAIRRKDKIQPTVTMSRKTSDKIQGVAFYGHYLLASRSYGPYTSELLVSER
                     DSPSRILKRIKFPPYLEQIVVVEDRLAVLFESGAAAYREKANPVLANVLLLDLATLLH
                     ANKRPKKIAATKK"
     gene            complement(37299..37703)
                     /locus_tag="SCJ86_001043"
     CDS             complement(37299..37703)
                     /locus_tag="SCJ86_001043"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292291.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="organic hydroperoxide resistance protein"
                     /protein_id="HEG1168621.1"
                     /translation="MKKMYETAMINRGGRDGEVEAPNGSMHMKIDRPGIHSEGTNPEQ
                     LFAAGYASCFNGAVQHMLEENNLESDSEVKARVSLFQLEDGGYQIGVVLEVSLPGLEK
                     DQAEKIAEEAHAFCPYSKATRGNIDVEVTIVE"
     gene            complement(37936..38571)
                     /locus_tag="SCJ86_001044"
     CDS             complement(37936..38571)
                     /locus_tag="SCJ86_001044"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF998 domain-containing protein"
                     /protein_id="HEG1168622.1"
                     /translation="MIFLQKYGFYFLLLGVMSDLSTPYILGLFYPKLNQMTTVISVFG
                     DVDSPVRRAFLVWSVVSGLFFVLSLPALYHLFVGTSKTLAILVVATVGLYGIGDCIFT
                     GLFSINTNESSCNLSTWIHNIGSGLGYAGFLLFPLLLVLLYRQSGQGTLSHFYLVLTV
                     ISLLIAGLYGLARIPSISQFAFFKQLGFFQRLSFFFNYLPMICLSFFQLRK"
     gene            complement(38837..39889)
                     /locus_tag="SCJ86_001045"
     CDS             complement(38837..39889)
                     /locus_tag="SCJ86_001045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853127.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HEG1168623.1"
                     /translation="METSQKISPKIFLNKVLAGTATGIIIGLIPNAVLGAILKYFSAY
                     PFAVTIAQLAVIFQLATPLIIGGLIALQFGFDPMRMMVVAGASFVGSGVVKFTPDLGE
                     TGVYVGAGTGDIINTMLTASIAVGLILLIGNSFGSVSIVLTPIVVGVGAGLLGFYLFP
                     FVTAITSAIGSLINNFTTLQPLLMSILIGCSFAFLIISPISTVAIGMAIQLDGVSAGA
                     AAMGVAATTFVLVVNSWKVNKSGVTIAIALGAMKMMMPNLFRKPVILIPCLVSAVITA
                     IPVALFSISGTPASAGFGVVGLVGPLASLDAGLNGFLVVLCWLVIPVFAALITQFLCE
                     KVFKLYDREEVFKFLG"
     gene            complement(39902..40840)
                     /locus_tag="SCJ86_001046"
     CDS             complement(39902..40840)
                     /locus_tag="SCJ86_001046"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015081805.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-dehydropantoate 2-reductase"
                     /protein_id="HEG1168624.1"
                     /translation="MKIAIAGAGAMGSRFGLMLHQSGNEVLLIDGWAEHVQQIKEHGL
                     QANFNGKEVEAKLPIVLQSEVEKEDQVDLIILFTKAMQLEKMLQDIQSLIKKDTEVLC
                     LLNGIGHEDIIEKFVPMENIYIGNTMWTAGLEGPGQVKLFGSGSVELQNLGDGKEAAA
                     KKLADKLSESGLNAHFSDNIHYSIYRKACVNGTMNGLCTILDVNMAELGKTSTAHKMV
                     ATIVNEFAKVAAVEKIELDVPEVIAHCESCFDPETIGLHYPSMYQDLIKNHRLTEIDY
                     INGAISRKGKKYGVATPYCDFLTELVHAKEDSLNVK"
BASE COUNT        13103 a         8093 c         6677 g        13454 t
ORIGIN      
        1 ataacataaa aaaccttttt taacggcatt tatatgcttt ttcagaaatt aactgatgat
       61 ttcaaaaaga aaacatgtca taatatttcc catattgtgc aaaaaaatac aaggcggagg
      121 ctctactatg attgaagaat atattgagaa aaatatttta agacagctgt ttttatgtgg
      181 gcaattctat gtaaataaag aagtcaatct agaaaagctc tccaatctcc tgcatgtttg
      241 caaaaccact ttactcaatg atatcaataa cataaaaaaa gaatttgaag aacagatcgc
      301 ttatacacat cgggaaaagg accgttacac tttatatttt tcagaacata tacctcgatg
      361 caagatcatg cagcaattgt ctcaaaattc gttgttttta aaaacttgtc tgctttattt
      421 ggaagaagat gaacctgatt atcttcaatt aactgaatgt gaattcatct ctgtcagcaa
      481 agcttattca ttaaaaaaac aagtattagc ttactttaat gactgcggga tcgaaatcga
      541 ccgctactcc cctcgattca ctgaaatgga aagacgtctg ctgcttctta atgtgtcgta
      601 tcgtttaggc ggttttaatt cttgggagct acctgaatcg ttttttgaac gtgccgatcg
      661 tttcattgaa tccgtaacgg aaaatagtgg cagattttat gataaagaaa ataaagaaat
      721 cttatcgatt ggttttgcta tcagtttcct acgccagcaa gtgtgtgctg tgacaataga
      781 ttcaaaattt atcgaagaaa tcaaaaaacg tcctatttac aattatgtcg aatcagcatg
      841 ggaaaacact gatttccaaa cctattataa aaaagaagaa ttcgccttta ttttgacact
      901 attcaatctt tgtaattatg gtttccattc ttatcagttg attgcggaag actttcaaca
      961 gctacaccaa gtgtttattg acaatactcc cgaaataaaa gaactagttg ctacctttga
     1021 atctcacttc aatcaagaat tattcggaaa ccaaccgttc gaacgtgcgc tgattcatct
     1081 gatgcgttcg gcttgggata actatcaatt attcatgcca gaaaaatttt atcttttaaa
     1141 tgaggaacag acaaatttat ataaagaagt ccagacaatt ttttccagct ggtccagcca
     1201 gttaccttat gatctacggt tgaatcctaa ttgcatgcgg gcttttgtga tcgaattgag
     1261 cggaattctc cgtctgacaa aagaacatct gactatctat atcgtcacaa attcggatgt
     1321 tcattatctg atttatcggg aagccttgga agctgtaact actttcgatt ttcaagttgc
     1381 accaacaatt tattcaagta tatctgatat aaagaagtat gcgcagcagt cctcaaaccg
     1441 ggtattatgt gaacgtacat tatatacacc agatgcggtc caatatgaaa atattattcc
     1501 tatttcgatc aatacgatcg atcgagctat catttcagct gtgcaaaaca aataaggggc
     1561 tgtgacaaaa atcatgtcac agcctcttat ttgttgaaga tgcccatttc tgtaacaatc
     1621 tcttgataca cgtcaggctc acaacctcac ttattttgga catctagggg tccgatctct
     1681 ttatttaaaa aataagaaat gatggttcgg ataactacca acgccgctag ttggaaaata
     1741 tcctgcaagg taggtttgat aatcgattcg atgatcccag ctgaaacaag aatttccaaa
     1801 cttagaagta tatagcctcc caacatcttt ttaatagcat tgttctcgct tactcggtcg
     1861 ctttgcactt ttgtatggac aaataaatat ttcaaaaaat ccttgattga tagacaaaca
     1921 ccccaaacca ataaacagat agcaaagata tcaagtccca gtacaagttt ttcgaaatat
     1981 ggaagaagaa actgcatcaa ctctgtcgtc atcctacctc tcctcttctt tgtttttctt
     2041 tgtcttctat ttcctgattc aagaaataag aaatgactgt tcgaatgata ataataagtg
     2101 caagcttcaa aatatctgtc caagtcggct tgataactga ctcgatgata tcagcaacga
     2161 ctagtacttc caagcttaac aaaatataac ttgctagcag ttttcttgta tctgcattac
     2221 gagtattacg ttctgcatag tttttcttga catgagacca atcaaaaagt ttgaatagaa
     2281 caagaacgat cccgacaatt aagataccga tagaacaaat atttaaaata gcaatgatcc
     2341 attctagaaa ttcaattaaa tttgagcgaa acatatacgc tacctacttt cttttaattt
     2401 gttcctattc ttttattgta aaagaaagtt accttgttcg tctatttaaa agttgttcta
     2461 atgataacgg ttcgcttaaa tttttctttt tcactctccc atttttctcc ctccatccat
     2521 aaatcgtgga agtatccaaa acaacgtagc aagttaaaat aaggcttagt ccgtcatcag
     2581 cttttcaaag aagcactgtc tcggtgcttt ttttgtaccc aaatttctaa aaatagaacc
     2641 atacacctcc tcaaatcttt tcttcttacc taccctattt ttatttacag gaaaatcaga
     2701 cacttcttct tttttgtcaa aatttccttt ttcttctcgt cctatcattt ggcattctaa
     2761 taaaaacgaa ctatccttgg atatagaaag agaaaacaag gaggcgtttt tcatgagtaa
     2821 aaaattaatc aaagtcggca tcggtttagg tttgcttgcc cttggcgctg catatctagg
     2881 aaaaaagaca ggactattcg aagatgacag tcatttatat gatgaattcg aatcaatcta
     2941 gcatcctatt tagttgaatc actacggaca tacttggaag acattggagg aatcacaatg
     3001 agaaaaagta aagcaataat gattggtgcc ggtattgcca atatggcagc ggctgtttat
     3061 ttgattcaag aaggcaaatg gaaaggggat cagatcacct tttattcatt ggatgatcac
     3121 ggttcaaatg atggtgcacc gaccgacact gtagtggatg aatattggaa taaaaaccat
     3181 ccattagaaa accaaaaagg ctatattgct cgaggcggtc gcatgcttaa ttaccgtacc
     3241 tatgtagatt tgatggatct gttaagtcga ataccttctg ttactgagcc agggatgact
     3301 gctgaagaag atacgagaga ttttgatgca aaacaccgaa cctttgacaa agcacgtttg
     3361 atggaaggtg gcaaaggaat cattgatggc ggaaaactgg gattcaataa taaagaccgt
     3421 attcttctga ctaaactgat tgctatgcct gattcagaag aagaaaaact agacaatatc
     3481 actatcgaag aatatttcaa agatgatcca cacttttttg aaacaaactt ctggtttatg
     3541 tgggaaacaa catttgcttt cagaacacgt agttctgctc aagaattgcg tcgctatatg
     3601 catcaaatga tttatgaatt cactcaaatc gaacatctag tgggcgtcaa tcgaacacgt
     3661 tacaatcaat acgaaagtat tatgcttcct ttgatcgagt acttgaaaga acaaggatgc
     3721 aaaatcattt tgaaccgccg tgtcactgac tgggaattca aagaaacacc gatgcaagat
     3781 gaaatcactg tgactggttt gaaaattacc aatacactga ctcaagaaga agaatacgtt
     3841 gtggtcgatg aagatacagc agttattttc actaatgggt ccattacaga ttcagctact
     3901 ttaggagatt acgaaacacc agctgtagaa aatatggatt atggtgcagc ttctagtctc
     3961 tggaaaaaag ccagtgaacg tttctacaac ttgggaaatc ctgacaaatt ctttgctgat
     4021 cgagatgcaa gcgaatgggt cagctttacc ctgacgacga aaaaccattt gttgttgaat
     4081 gaaatcaccc gtatcacgac tcaagaacca ggaaatgcct tgaattcatt tatttcaacc
     4141 acaccgatca cgccgttagg ccaaaaagat gtcaatatgt ctattgtcgt gcatcatcaa
     4201 ccgcatttta cgtctcagaa accaaatgaa acagttattt ggggttattt cctttatcca
     4261 agacgacgtg gcgaatttgt ggataaacag tatattaaaa tgaatggaaa agaaatgcta
     4321 gaagaactga ttggacaact ttcaaaagta gatcctggac cagtgaatat tcgtgaaaaa
     4381 gaaacagaaa ttttcgacag cgtaataaat aatatccctg tctatatgcc ttatgcttcc
     4441 gcgttgttca ataatcgtgc caaaagcgat cgaccaaaag tcattcctaa acattcgacc
     4501 aatctggcct ttacaggaga atttgtcgag cagccttatc agatgatctt tactgaacaa
     4561 agtgctgtac gttctggaga aatcgctgct ttccattttg cgggtattcc tatgtcccgt
     4621 cttgtcaaaa caccgcgtta cgacaaagat attcctactc tagctcgtgc cgcaaaaaaa
     4681 atgtttgaat aatcaagagg ttgtgaaaaa gctgccctgc atcaagtaat aagaacaacc
     4741 aggctaaaac agcttcttat gtttttgatt tggttggact tattaccgca gatgcaagct
     4801 tttgaacacc gtttaccaag aggttatgaa aaagctgccc tgcatcaagt aataagagca
     4861 gccaagctaa aacagcttct tatgtttttg acttggttag acttattacc gcagatgcaa
     4921 gcttttgaac accgtttacc aagaggttat gaaaaaggcg tttagtttcg agtatgaaga
     4981 aaaattttgt gaaaatggtt cctcacgttt ttgcataaat ttgacttaat accgagaaac
     5041 tgatcccttc tgtcataacc tctttttatt tatgtctgtt tctttttgaa aacaactatt
     5101 ttttcaacgt ttactctctt gccaatccaa catcatctaa cggatacaac tatttcaatg
     5161 attaccatta cactaaaaaa tcggaaacac acctctccga acacaaatac ataagactat
     5221 agaatgtttc atttaagaaa aaaagcgttt cattatcaaa aaggtaagat ctcctttaaa
     5281 atgaaattat ctgtagtact aataatgcag acaattttat gaatctgaaa ggagcatctc
     5341 caatgaaagc agtcacttgg caaggaaaac agaaaatgga aatcagaaac gtagatgatc
     5401 caaaaatatt agaaccaaca gatgcaatca tccgtatcac agcaacagcc atttgcggct
     5461 ccgatctgca tctttaccat catggtgaag cagttttgga aaaagggtat gttgtaggac
     5521 acgaaccaat gggaatcgtt gaagaagtgg gatctcaagt tcaaacattg aaaaaagggc
     5581 aacgtgtggt tatccctttt aatattagtt gtggtcattg tcatttctgc ctgaatcaca
     5641 tggaaagcca atgtgatgaa tcaaatccag atcagttata tggcgggctt ttcggatttg
     5701 gcaagttgaa cggtaatcat tggggtggtc aagcggaata tctaagagtt ccttatgcag
     5761 actctacttc atttgttgta ccagataatg atcttcctga tgaaaaagtt ttatttctat
     5821 cagatatttt gcccacagct tattggagcg ttgaaaacgc aggagtaaaa aaaggcgata
     5881 cagttgtgat tctaggttct ggtccagtcg gtctattcgc tcaaaaattc gctgttatgg
     5941 ctggagcaga gcgcgtaatc gcagtagatc cagtacagca ccgtctagat aaagcggcta
     6001 attacaatgg ggttgaaact tatttcttag aagacacggc acaagctgga acagaattgt
     6061 atgaactggc taaaggtggc gcagatgtca ttatcgattg tgtaggaatg gacggattag
     6121 agcctgtcaa agaaaaagcg aaaaatttag taagcttaca aagcggcaca atctctccat
     6181 tacagatggc atcgcaagct gtcaaaaaat ttggaacagt ccaagtaact ggtgtttata
     6241 tgactccagc ttcttcttac ccgctgcaag aattttttat gcgggatatc gctgtcaagc
     6301 atggacaggc accaattatc catctaatgc caaaaattta cgcaatgatt gcagaaggac
     6361 tattcgatcc tacacaaatc attactcatt cgctgccact atcagaagca gcacaagcat
     6421 atgagatctt tgacaaaaaa gaagataaaa atataaaagt tgttttgaaa ccttgaattc
     6481 agtaaaaaat acaggtgatt ttctttatgc ccaaaatcaa acgaaatacc catgagatcg
     6541 aaataggaaa agaagaagat atcgaaatac gaggacgtcg cttccgcttc tatttccaaa
     6601 atcgttacac attgatctca ctggctgtcg atttattaac cgggattttc tatatttttg
     6661 gaagccttgc ttccttaact aaaatccccg atcgatatgg cacctatttt tatttggctg
     6721 gtgctatttt cttgacgatt cgtcctattc tcagaatcgt aaggaacatt tttatttacg
     6781 acgaaaaaaa agcaatgaag agaacaagca aaaaaaaaga ggcatgagaa tttttcaaat
     6841 ctcatgcctc ttttttcacc ctatcgtatc actttgatac agacgagcgg tcgtcccact
     6901 ttcttccttg accattgtca aaaatgtcca ctcgtatcgt tcataaaacg tggtatgatc
     6961 tgtaattaag taaagttttt cgtacccaag ttgatgcatt tcttttctga tttccgctaa
     7021 tatccgcttg gctatcccct gcttacgctt ttccggttca acaaacaggg cacaaagatt
     7081 tggtgtcaga tcagggcggt tatggaagtc gttttcaatg acgcccgctc ctccgacgat
     7141 ggtttctttt tcatctaaga ccacatacca ttgaggaata ctttccttat tagcgattcc
     7201 agtacgaata ctttcctcat acacaacagc aggaactccc cattttgatg aaaaccaatt
     7261 cgcgctttgt tttactaatt ctgggtgttc tcttaatttg atccagttat atgtaaccaa
     7321 tgtttaaacc tccgactaaa ataaaacgtt tacaaaaatt atacaagtct ttatgaaaat
     7381 tagcaatgac aacaatagtg atgtaaaaaa aaaaacggaa aacaatcact tgttctccgt
     7441 tttttgatta atcaagcatc taaagcttct tttcttcttt cttggatcgt agctaccttg
     7501 acgaacggta gatagatcaa aatagaaaga accaaattga cagcggctag tacaccacct
     7561 gcaatacttt ttgttgccaa tacaccgcca ataattggtg gagttaccca tggaggcata
     7621 acggtagctg ctggcactaa acctgtagaa gtcgcaaagt aagcaacact aactaagacc
     7681 attggtgtca aaataaatgg aatgaacatg atcgggttca acacgatagg taacccaaac
     7741 atcgttggtt cattaatatt gaagacacct ggcgcaatcg acaagtttgt tacaaccata
     7801 tatggtttgt ttttacgtcc tactaaataa atagctaaca gtaaacctaa agttgcacct
     7861 gttcctccta agttgacaaa gctgtcaaag aaaggtttgt tcacgataaa cggtgcgcta
     7921 tgtccagctg cgattgcatt aacgtttgct tcaatggctg gcgcattgat tgtttgcatc
     7981 aaaggatcga ccatattcgc tccgtgtaaa ccaaagaacc ataagaatgg tgtgataaat
     8041 gcaagcagta aggcagatgg ataagagttt gcaagtccca taaatggttc ttggaccact
     8101 gtatagaaag aacctacgat atcatcaaca ccgaatgcag caaaaattgc agcaactaaa
     8161 gcaaacgcag agatagtgat cattgctggt agtaatgaag caaatgcttt tgcaactgcc
     8221 ggtggcacac catcaggcat tttgatgatc aatttttcat ttccgcttaa acgtacaaag
     8281 atttctgttg aaataatccc aacaataata gcaataaaca aaccgtttga gctcatacct
     8341 aatgctccgc ctaaagcgaa gaaagagcct aaagaaatca cacctgcgga aacaccgtct
     8401 tttcggtagc cgttagctaa atgataagca agcaagaaag caatcaagac agaaagaatg
     8461 gcaaatgtac cgttccaagc agcagccccg aatcctttcc atgattcacc accaaaaatt
     8521 gagttcatca attcttggaa cccgggaatc ggtaagttat tgatcattac tgctaatgct
     8581 cctaagatca ttaacggcat actcaccata aatgagtccc gaatcgcaac taaatgccgt
     8641 tgtgcaccaa tttttgatgc tacaggtata aaatgtttct ccataaatgc aacaaattta
     8701 tccataaatc ttccctcttt tctttactgt agattacgcg ttcaatcgac gatacagatt
     8761 aatgatctct tttgctaaat ctgtaaaagc tatagatgtc atcagatgat cttgactgtg
     8821 gaccgtcaaa agcgtgactt caacgtgatt tccttgtgct tcttgtgtca gcatctctgt
     8881 ttgagagtga tgtgcttgga ctaaagattc ctctgcatct ttgatttttt catctgctaa
     8941 agtgaaatca ccttttttcg cagcagcaat cgcttccatt gcatcgcttt ttgcatttcc
     9001 gccaaacatg atcagaccca tgatggcttc tagatttttt tgatcttcca tttattgtat
     9061 cctccgataa tttttattat ttgtttatta gtttttctgc ttggtcgagt actttttcac
     9121 cattcatcat gccatagtca gccatattga ttacatctaa aggaatgcct ttaggtgcta
     9181 gcttttcagc aaattgattt tgcataaagc gtacttgcgg tcctaaaagt aaaacgtcta
     9241 catttttaga ttctagttga ttatccgcat ctgaagcaga tacagcaaag atgtctgctt
     9301 ccattccttt tgcttctgct gccttttgca tttttgtgac taggagactt gtactcattc
     9361 cagctgaaca tacaagcatg attgttttct tttccatttt aaacacgttc ctttctatac
     9421 ttacacttgt atataagcaa gtagcgtgcc aactttaaaa gcgctttaaa aagttgatat
     9481 catcacattt aaggtctata ccaagttgca tacacactaa aaaagaaagc gcacactaaa
     9541 cacacaaatt aatttttatt accttttttc ttttaacgtg cacaaaaaaa gttgtgacac
     9601 aaaacttgtc acaacttttt tcaaataaac ggtattcaaa agcttgcatc tgcggtaata
     9661 agtccaacca agttaaaaac ataagatgtt gtttttactt tgctattctt attacttgat
     9721 gcaggacagc tttttcacaa cctctcaata aacggtgttc aatcagctga ccctgggtat
     9781 tagctcaaaa cgctggattc acaacctctt ttatttcaaa taaccagtaa atacaatgaa
     9841 catctacaaa aaacttctat ttatttatag atttcgtcag gcaaatccaa ttctctctta
     9901 tcaatcaaag tctgatacca cgaagcagat tttttcaatg ttctgcttcg ttcatcgttc
     9961 aattcgattc gaactaatcc atatcgattc ttaaatgcat tcattggtga cacacagtca
    10021 gtgaatgccc ataacatata gccttcgcaa tttgcgcctt cttctgttgc tttgatcaag
    10081 taatgcaaat gctgtccgat aaattcgata cggtaatcat cttgcacgat tcctttctca
    10141 tccatatact gttcttcata ttctcttccc ataccgtttt ctgttatcag ccaagggata
    10201 ttatgataat tttcttttag atacatggct atatcataca tgattttcgg atagatttcc
    10261 catcccctgg aattattcat tttcttccca ggtaaatcaa agacttcata atatcgttca
    10321 ggatggaaag gtgtctcttc attccattga taacgagggg cgcgaacgcg tttaggataa
    10381 tattgattga ttccaaggaa atctactgta tgatgctgaa tcgtttctaa atcctctgtt
    10441 gcaggatcga aataaatcga ttgtttttga cacaactcca ggaactcttc tgtgtagtat
    10501 ccttttacca tcggatcaaa aaatactcga ttataaaaca aatcatacat ataagcagct
    10561 ttttggtctt ctggagaatt ggatcgagca tagaccattt caggatttaa aatacagcca
    10621 atccttccac cataatttcc gctgcggtaa atctctactg ctttagccgt tgctaaaact
    10681 ttatgataat tccacatcat ccactttttt gtgttttgtt catgcggcca gcgaacagca
    10741 tccaaatagc aacgcgtttg gggaacgatt ggttcgttga atgtaaacca gttttttacc
    10801 ctatcaccgt aacgatcaaa ggcaatttga gcatacttag cataaaaatc aactactttt
    10861 cttgaagacc agccatcata tttttccaaa agaaaagctg ggacttcata atgttccaaa
    10921 caaatcatcg gttctacgtc tgcttcgatc aaagagtcga tcatttcact tatatgctga
    10981 gcatattctt catctactat acagttctca taatcagtaa aaaaacgagc ccaattgata
    11041 gaagtccgat aatgtgtcag atggatggat ttcatcagat ccacatcctg ctggtattgt
    11101 tccataaaat tagttgctac ggctgggcca tacccattat gccaaacaaa atgttcattt
    11161 ttataccagc tatctataaa cgaatcctgt ccttcttttt ttcctttcca accttccgtt
    11221 tgccaagcag aagatgcagc accaagaata aaattttttg gaatagtaat tttcatgatt
    11281 tcctcctgtt ttttagatcc catcattttc cacacgattt gctactttta caaatggcag
    11341 atagatcaaa atagaaacaa taatacaaac aagttgcgta ataactgcac cgatatttcc
    11401 tgctgttgcc agataagcat tgacgatcgg cggcattggc caagggacca ttacaactgc
    11461 tttcgccgca aatcctaaag cggttgcaaa ataaccaatc gttccagtga taagaggcgt
    11521 taagataaac ggaatagcta aaattggatt cagcatgatc ggcataccaa aaataactgg
    11581 ttcattgatg ttgaagatcc caggagcaag ggataacttg gtgatttgtt tcatatcttc
    11641 ccgttttcca actaacaata ctgcaatcag taaaccgatc gtgactccag acccgcccat
    11701 actcatatac atatcccaaa atggcatagt aataatgttc ggtacttctt ttccagcttc
    11761 aaaagcttcc gtatttacag caattgccgc taaaagcaac ggttcccgta tcggcttcac
    11821 catttgattt ccatggatac caatcaccca aaagacttgc gcaataaaca tcaatagcaa
    11881 aatacccggt aacccttgta cgacaccttc taatgggcgt tgtacgacat tgtaaatgat
    11941 atcataaagg tacattcctg taagttcttt gatgataaat cccgcagccg cgatacccga
    12001 tacagtcaga atcgtcggga ataaagcaga aaaactagca gaaacattcg ttggcacggt
    12061 atcaggcatt ctcagctgta gtttcttctg tttcctcaac cagcaataaa gttcaataga
    12121 aaggatggag accaacattc ccaaaaataa ccctttcgta tctgtatatt gtttagccaa
    12181 tacgttttct actataacag tctgattttc attcactaat tcataaacag ttggattaac
    12241 agataaataa gacatcactg cgatgatacc agggaataca ccatccactt tattcagctt
    12301 ggctaattcg atccctatca gaaaaacagc atagatggtc aagaaactta gtgtgacata
    12361 gctgatggca gatgcgatag gctttaattc ttctaaaaaa cgcaagaaac ttatctgtgc
    12421 taagccattt tccgagtcaa agaccattgc tgaaaacaat gtgccaaaag cacccgtaat
    12481 aattaccgga atcaatgcag caaatgcgtt ttttatcacc atgatatacc ttaaattatt
    12541 gattctcgtt gccacgttcc ctaaaacatt ggctaattta tccataaaac tcattccaga
    12601 tcccctcatt cctgcttctt ctccttgacg tcatctcttt ttgtctatta gtcagcgctt
    12661 acatttcgat gaacagaaac tatttactcc tcttttttac caaatataaa acaaaaattc
    12721 atctgatgta tttatcataa aatacatcag atgaactgtc aattagtttc tttttctcct
    12781 attattttct gtaattctct ccgatttatt gccatatgga cagtcattgc atcaaacgca
    12841 gcaagtgcat tccgcctttc gatggcctga acgatctctg tatgaatatc gatcgtctcg
    12901 attttcagat gctggatatc ataattttta ttaaacaaag caattgattc attaatgatt
    12961 gggataatat gcagcaaggc tacgtttccg ctagcttctg caatcatcgt atgaaaatcg
    13021 atgtctaatt gaacatgttc ttgtgtgcct tctcgaaaag aagtttcaat cttttgcgtt
    13081 aattttttca tttggacgat ttgattcttc gttgcatatt ccgctgccaa acctgccatc
    13141 ttaggctcca ataaatatct cagttcaaat aagtctcgta tcatccgatc ctgatcttca
    13201 atcaaagaaa aaccgagtgg gtcattggca atccctcgct tatcactgac aaatgtcccc
    13261 gctccttgcc gaacttcaag gatattcctg gaagctagag cccgcacagc ttctcgcacc
    13321 gtgcttcgcc ccacctctaa ataatcggac aattcatatt cattcggcag tttatctcct
    13381 acttgcaact gcatttcctt taaatatcta aggatcttgt cagtagttac ttctactagg
    13441 gttttatttt cttccaaacc acatacctcc ttttcatctg atgtattttt tcgtaattat
    13501 tataacacgt ttgaatcatc tgatgatttt ttgtatacaa aattgattaa tccgttcata
    13561 gacatacaaa cgaagaagat atttaccata aaaaagacta ggataaaatt cactcctagc
    13621 cttcgatcat ttttattttg aacgacaaaa aatatgatgt tcaataacag acgatcttca
    13681 ttcgattctt gatgtcatct tttgtttttt ttaatatatg gataaaaagt ttgattacat
    13741 cgtttctatt ctagaatttg gtcgattctc aattcttctt ctggtgagaa atctaaatta
    13801 tccatcattc gaacactttc ttgtaactga cttaaccgac ttgctccgac cagtacactt
    13861 gtaacagatt tttgtcgcaa attccatgca agggccattt gcgctaatga ttgtcctcgt
    13921 gatacggcga ttgtttgcag tgctttgatt ttttccagag ttgatccaac ttgttcttct
    13981 gaaaggaatg gtatttcctt acgatgtgct ctagaatctt caggaatacc atgtagataa
    14041 cgatttgtca gcaacccttg agcaagcgga ctgaacgtga ttgcccctag cccttcttct
    14101 tctaatactg gagatagatc gtcttctagt agtgcacgag aaaacatatt gtagcgcatc
    14161 tgatgaataa tgaacggtgc ttcctttctt tttaaaattt cagtcatttt ttttgtatct
    14221 tctccattat agttggatat accaatatat aatgcttttc cttgtcttac aagttgcatc
    14281 aaagcttctg ctgtttcttc caaaggtgta tcagggtctg ggcgatgatg atagaagatg
    14341 tcaacataat ctagccccat tctttggaga ctttggtcac aacttgaaat gatggattta
    14401 cgagagcccc attccccata tggcccttcc cacatatgat aacctgcttt gctagaaata
    14461 atcagttcat cacggtaggc ttgaaaatct tctctaaaaa tacgcccaaa gtttttctct
    14521 gcagctccag cagggcctcc ataattattc gcaaggtcga aatgggtaat tcccatgtcg
    14581 aatgcgcctc ttaaaatctc tctttgatta tggataggat cataatcacc aaaattttgc
    14641 cataatccga gagataaaat cggtaatttc aaacctgaat gaccggattt acgatattcc
    14701 attttttcat aacgtgcact atctgcaata tacaattttt ctcctcctca tcaaatcttt
    14761 ttaaaacgct ttcttttttc tatttttagt ttactacatt ttaaagaaaa ggagttaatc
    14821 aaaatgattt tttcgtatat ttttttgcca tttgtttttt actcgttgat agatcagaaa
    14881 ttgattacgt gcttgctgga tttttttata acgtttcggt tgttttttgt aaaattgctg
    14941 atgatagttt tctgctggcc aaaacgtacc ggctggttcg atttttgtga cgattggttg
    15001 gtggaaagtt cctgaactgt ctagtttctg ttttgattgt tccgctaatt ctttttgtcg
    15061 ttcgacttga taaaaaatca ctggacgata ttgcttccct ctatcttgaa attgtccaaa
    15121 tgtatctgtg ggatcagtaa tttgccaata gattgtcagc aactcgctat aacttatttc
    15181 tcttgtgtca taaatgatct ccacagcttc cacatgacct gtgtaaccac cacttacttg
    15241 atcataattt ggtttttcaa catgtccgcc cgtgtatcct gacaaaacag ataggatccc
    15301 ttttttcgat tcaaacggct cgaccataca ccagaaacat ccccctgcaa atactgcacg
    15361 ttcttgatag tcagcatctt gcatggcatg cttggaaaag tcatattttt gctccttatc
    15421 aaatccttcc gtaatcaata aataaaagtc cattacatca ggcgtcagat tcattcggat
    15481 agctaatgga cgtagtgttg cttctagttt tgataactgt tctttaacgg atatattttc
    15541 tcttatgtca ttctttgtct gaatcaatac ttttcgttcc caatctcgtg tattcggatt
    15601 caaaatcaga ttataaagtg tttgaatgaa tatttgttcc tcatccatag attctcctcc
    15661 tttccgaaga agacaagcta gtgactttag ctgtaattta tcataaagtg aggaaataag
    15721 cgattacttc atccccctac agatttttcg tataaaaaag agagaatcct tatcgacaga
    15781 ttctcctttt taatcaattt attttataaa tgaaaagtta atttaccttt tatggaaaac
    15841 tattttaaaa aataaacata aatatgcagt taatgtaaca agataagaaa taatagtcgt
    15901 tactgtataa cttgtgctta ctttaatatc agcaactcct ttgactccgc ttatttgtac
    15961 caatccatta gcattcacct gctcctttac aggttgatga tttatttcta cattcgttcc
    16021 taaataagca tatacaggaa gtgtgattac ttggctcttc tcattagtta ctctatatat
    16081 gatagaacta ccagttgttt tataaggaag gtttacccat tgactgccaa tctttgcttc
    16141 gtgttttatt atatgctcct tattctccaa tgaagcgata ggagaataat catataaaaa
    16201 ataatccatt cctttagtgg tatacgcctc tactgtttct ttgtttacaa caatatgtcc
    16261 cggctgagaa attatttttt gattgaaatt ttttttacta gaaacagtta aagtgattaa
    16321 taacaaaaca gtcataaata aataaaatag ttctcttgtc ttattgtatc ttctattttt
    16381 gaaaaatatc atcgttaata tcagagaaga gaatattatt tgcaagctta agatccgcca
    16441 aggaaattgt aacaatttgg ctggcgtatt ttgcagaaga taccatggca ccaaactagt
    16501 tgtagctagc caagtcaaac acgtaccata ataaatcttt ttttctatgt ttgttaattt
    16561 tttaaataaa atcaggggaa tacataaaga taacagacca actagtccca ttgagtaagt
    16621 agttaagtcg ttgttcatag cttcagtcag actatcgaac acattagatg catgagcata
    16681 taaatttgtc acgctaggtt tgttgatttt ttggtagagc atttgttcaa acatcggata
    16741 ccaaaaagca ctcgtcagaa ccaatgtcat tcctgccgca ctaaataatt ttttaaatat
    16801 agcaataaaa tctgcattct tgaaaccttt agaaaccaaa cgaataaaaa taaaaatgat
    16861 aattaatgct gtactcatta atgttgatag tacatggctg tacactagca aactcatact
    16921 caatgctagc tttacagacg gatacttttc ttcttttcca gagatgattt gataagcata
    16981 aaataataca gggataacaa gtgttattgc gatagcttcg gcaattgcgg aacgatagta
    17041 gatgtctact gtccgataag cagagaaaat atagagacaa ctaaataaaa atgccgcttt
    17101 tttttgtttt aaaaaccgct cgccataatg ataagctacg agtgttgtac acaagttcaa
    17161 taaccaaacg taaataatat agcttacaat caaatttttt gtcatccaat ataacattac
    17221 agctggaaaa aaagtcagaa agggataaaa ataatttaca ccatatccag tataattgaa
    17281 tgtctcataa ttaatcggac tggtaaaaat agtcgataac cccttgattc gagataagtg
    17341 aaaagaaaga tctccagtgg tataaatact ctgtgtattt ttgaaaaaga tgaaagaata
    17401 tatactacta actataatta atccactcaa tattactact tttttattta ttttcattct
    17461 tcttgtttat ctccttttta gtcacatatc gagaatcttt caacttatta ggtgcaaatc
    17521 gtttaaaact tgaatacacc actaaacaat aagtcagaat actgatccat aaagccatga
    17581 gagcagttgg agtatatttt gttgtaattt ctatcttatt ttctccagca tttacatcca
    17641 cattcactaa gccatgtttt ttataagagt catttacgac tttaccgttt acttttacct
    17701 gtgtacctaa ataagcatat actggtactg tcactttttg ctttttgttg cttgccactg
    17761 tgtaagtaat gttattatct gcagctttat acttcccttt ttcccacgat tctcctactt
    17821 ttacttcatg tttttctaag tgaggccttt cttttatcgc ttctgttggg gcgtaatcgt
    17881 ataatcctcc catatctgat gttgtataac gtgggagtgt ttcttcattg ataacaatat
    17941 gaccgtgttt tgattggata acttttgcgt aattttcttt tgctgctata gacgtgacga
    18001 gtagaaacac cacagtagcg ccaatgatta accatgaata ttttttattc atagtctttt
    18061 tcataaaaac aagcgttatg atcattgaag aaaaaaagat ttggatacct aagatacgcc
    18121 aaggaaactg gagtaagctt atcggtgttt tttgaaacaa aaaccatgga aataaattag
    18181 ttacgcatac ccatgtagaa actacgccaa catagatgat tttttctttt ttctccattt
    18241 ctttcagtat gaataaagga aaaatcaacg caaccaatcc taataacccc atagtgtatg
    18301 ttgtaatatc gttatttaac gcttgaatga tactatctga aatagaaagt gcttctagct
    18361 gcaaatctgt tttgaaggga ctgtgtattt cttgataatt catctgctgc aacataggat
    18421 accaaaaata gctagttaat gcacaagtca aaaaaacagc ctttactaaa ttcacgacca
    18481 caaattttat atcgtttttt atttgcttgc ttaaaaacaa acgaactata atcatacaag
    18541 caatcaatgc actacacaat agtgtagtta agacatgagt atacacaagt agacttatac
    18601 tgacagataa agcgatgact tttttttctc tattgaatat caatccataa gcataatata
    18661 aaacagctgg gataatcgta agagcgattg cttctgcaat tgcggatcga taatagatat
    18721 caactgttcg ataagttgca aaagtatata aacaactaaa taaaaaagca gcatctatct
    18781 ttttaaaaaa cttttctcca taataaaaac taatcagaat cgtacaaata tttaataacc
    18841 aaatatatag aacataactt aatattaagt tatttgaaat tccataaaaa acgactgcag
    18901 gaattattgt aagaaaagga taaaaatagt tcagtccatc tccataattg ttaaatgttg
    18961 tatagttgat tggcccttca aaaatagaag ataacccttt gattcgtgaa agatgaaaag
    19021 ataaatcacc tgttgtataa atttcttgag catttctcat aaaatttact gaatataaag
    19081 tactaagtaa gataagaact actaaaagaa atgctttttt acttattttc atagatttgt
    19141 tgttctcctt ttttaattct attttcagta tactagaatt gtcttttttt aataagtttt
    19201 gcgttaaatt tttcatagca ctagtatgat atactagaaa agaatcaatt tttattactt
    19261 ttaaatagga gaaaaaaatg aaaaaaaaca aaaaccaaat aatagatatc atttttatgt
    19321 tatttctaag ttttgcttac gtcatccctc tattcatgtc taagggaatc tatcatagtg
    19381 tcaatcagga tacttacttt catttatctc gaatcatagg actagataat gtatggagtt
    19441 ctccagtaaa cttcaataac ttcgatcatc atggaacaat gatgaatatt ttttatccat
    19501 ggcttactct ttatccagct tttttatttt ataaaatgat gggaaatctt gttttaagtt
    19561 ataatatcta ctattttttt atcacttttt taacaatggt cgtttcttat ttttctatga
    19621 aacaaataaa aaacaatcgt tatatttcgc tgttattttc gattatttat acattttccg
    19681 cctatcgtgc cattgacata ttccatcgtg cttcattggg agaagctgtc gctttaacat
    19741 ttttgccttt gatactaatg gggtgttacg aaatttatat tagagattac caaaaatggt
    19801 actggttgtc tattggcatg acgctagtag tgtatactca tttactttct gtcgctatgg
    19861 tttctgtatt tattggagga acgttgtttc tttcttttta tttttgggac caaaaaatcg
    19921 ctagactctt aagtctttta aaagctaccg tcctcacctt ttttttaagt gcgggattct
    19981 taattccttt tattcaacaa tctagagcac aagaattgaa agtcccactg ggaaaagaat
    20041 taagtggtat ggctcctagt gatatgctga cacatatttt aaataataac tataacaact
    20101 atactattgg attattctta tttctcggat tgattggtgc ttttattttt ataaaaaaat
    20161 taactgctga tgatttattt attttctttc ttggtgtatt tgttcttttt tgttcaacca
    20221 atttatttcc ttggcaactt tttaaccata caccagttaa gagtctacaa tttgtctggc
    20281 ggttaaatgg tttttcttct ttatttatag cctatacgat gagtatagtg atctattata
    20341 ttttttctac taaaaataat tcatggaaaa taatggtctt tacgtttctt gcactaatat
    20401 tgcatctctc aggagtaagc aattctatcc atgctaacaa agattcatta acacttatag
    20461 cacctgaaga cgctgttgct atagctgaaa attacaatca tactgattat gcaaacaaag
    20521 agtcaatcta tcatcctgat atgattaata atgatcaata tttactaggt aaccaggaaa
    20581 taaatccaac aactcatttt atgagtaaca aacttacaat tgaattagat aattcgaata
    20641 ataaaaatac tgtcttgacg acacctattt atcgttataa agggcaagtg gcttctatta
    20701 atggcaaact agtacaaaca aaattatcta agtttggcac aactgaatta actattcctc
    20761 caggtataaa taaagtcgtt attacttatc agtacacaaa actagcaatt gcttcaagat
    20821 atttatctat cgttactttg attttatttt tactttatcg atttactttt tctaaacaaa
    20881 aacaaccgag aagagaagta caacattctc actagatttt tatttaagaa agacttttta
    20941 gctgtacaca taaacaggct aaaaagtctt tttttatatt tctttaattt gcttcataca
    21001 ttatttaaca tcgatattta tactgtgaaa catgcgtttc atccattatc aaagtatttt
    21061 tatcgataat aaacttcatc caaaaacaga ccttgtgcag ggacggtttc tcctgcatgt
    21121 tgccgcactt tgctttcaaa tatttcatcg attgtttcta tcggcatggc acctgtgccg
    21181 atttctagta atgtccccat gataatccgc accattttat ataagaatcc gtctccgaca
    21241 aaagtaaaat gaagcatttc tccctttcgt tcgatagata attcatcaat cgtacgcact
    21301 gtggattttt ttgatttttt caatgcagaa aaaccaatga aatcatgtgt acccactaat
    21361 ttttcgcaag ctgcattcag cttgtccata tccaactctt gagggaaata aaagctgtgg
    21421 tttcgctcaa atgctgaagg aatcacgttg ttccaaacat aatagctgta ttttttgccc
    21481 accgcattat atcttgaatg gaagcgctca ggcatttctt cgattctttt gatgacaata
    21541 tcccgcggaa ggtaacgatt cataaagtcg atcatttctg ccagtccaat ttctgtgttt
    21601 gttttaaaat ttgctacttg tcctcgcgca tgggtgccag catctgttct tcctgatcca
    21661 ataatttcta tctcttcatt tgtcatctga tgcaatactt tttcgatttt tccttgaatg
    21721 gttttatcgt catctccaag tctttgccat cctgaatatc tttttccgtc atattcgatt
    21781 gtcaacttga tgtttctcat attttagtgg ctcctctgtc ttccatttgg tcttctctct
    21841 acctttttaa gattgtagca caaaatatag ctccttgctt ctctaagaca gaagctatac
    21901 aagaaaactg tggaaatata tatttcagaa aaacaaaaaa cagccaccgg atgaagtatc
    21961 cgctgactgt tcaatttgac ggctcaagtt ttactgtttg agatgcaaga tactcaacct
    22021 ggcaatgggc acataattaa agaaaaaact gattctttgg caaaaaccta ttttattcag
    22081 taatggccaa gaatttatag aaggaattct tgccagtcca taaatcgccg caaaaagcaa
    22141 actgatcaca gacagtccaa ggtataagtg gcttacgtcc atttgcccat tttttcgata
    22201 gatcaagaaa agaagaactg gaaacaataa aaaaccactg taacctaaac ctgatccgat
    22261 attatggacc catgtcgaga cattccacga agattcatgg gtattgatac tgaacaaacc
    22321 tgtaaagata caatcaccta taccatagaa cgcgatggct gctgtaacta gggtagctaa
    22381 cacacgagat gtactaacaa atgcagcata caatgcaggc aatgacagga tgaaaaagat
    22441 ccctgaaacc acagaccaaa taagaaacgc ttttcttaca ggactggaaa cctctccaaa
    22501 tacactgatc acgctcttca tttgatctaa ttcaggataa tacaatccta atacgtacgg
    22561 agtcaaaaaa tcactcagca cgcctaataa taaaaagaaa aaaccaaatt tacgtaaaaa
    22621 atccatgata cgcctccaat aacaattacc tatgctttta attgttatta taaacgactt
    22681 tatcttgatt taacagaaac ctctatttac acgaacatct atataactca ttaaaaactc
    22741 gaatcatttt aaaaatacta aatatctaac gttcaacgtt cacttaatcc atctaaacaa
    22801 tctttcaatt tgattttttc cccacttctt ttacgcgcgc ctagctctcc tattgcagta
    22861 aacaacagat cacttgacga atttaaggca gtttccattg aatcctggat aacaccgata
    22921 ataaatccaa taccgacgat ttgcatcgcg acatcattag agataccaaa caaactacat
    22981 gctaatggaa tcaataacag tgaaccgccc gcaacacctg aagctccaca agcagctaaa
    23041 gcagacaaaa tacataaaag gactttcaaa acaaaaggtg cttcaatccc taaagaatgt
    23101 acagcagtca acgtcatcaa ggaaatggta atcgcggcac cgcccatatt gatcgtagca
    23161 cctaacggga tcgatatgga ataagactct tttgtcaatt tcaatctttc agccaacatc
    23221 atgtttatcg ggatatttgc ggcagaactt cgggtaaaga aagcgggaag tgcgctttct
    23281 ttcaaacaaa agaaaatcaa tggataggga ttttctttaa taaacaaaaa cgtgaggaat
    23341 gggtacacaa ctaatgcgac aaacagcatc gtaccgatca aaacaagtaa aagctgcaga
    23401 tactctgcca atccagcgat tccagtcgta gcaattgatt gataaaccaa tcctaaaatc
    23461 cctatcggag caaatgcaat aatcacttgt acgacttgtg acaacgctat agatatctga
    23521 tccaccaact cttttgtcga ttctgaacgt gctcgaaaag ctagtcccaa caatacagcc
    23581 cagaataaga cgccaaggta attcgcatca ataagagatt gaacaggatt ttgtgtgaca
    23641 ttcgtcaaca tagtagataa aacactttcc aaactttgcg gtgctttttc agatactgtt
    23701 tcttgtaaag taataggaac agtaaaaatt gaacttacaa taactgctac aagtgcagaa
    23761 agcaatgttc ccactaaata taagatcaaa ataggcttca cgaatgtctt attcccaatt
    23821 ttatgcttgg caattgacgc catcgtcaag aaaaaaacca gaataggtgc gattgccttc
    23881 aagcaactga caaacaacgt tcccaatatt gaaataaatg tccatgtggg gaaaaagata
    23941 ccaaacaata ttccgactaa aataccaaca acaatacgtt gaactaacga catatccaaa
    24001 aacttctcta acaaatgacc accctttcac aattttaaca atcattgtat cattttatgc
    24061 aaagaaacta aaggtataat tttctaaaaa ttctaacaat acaaacaacc atttcttgct
    24121 aattttgtac tttttctgtc tacacgttgt tttttgagat attctaagtt aggatgttat
    24181 taatgggcaa cttatcagga aacgttttct tatgtttttg tccaattttt ttgtgaggtg
    24241 tttattcttg aaaaaaataa agaaggagag ttatggcttc ctgacaatct ttgtcgcttg
    24301 tataatgatg gttttacagc gtatattgat ttcagcagtc actattccta catatgcaac
    24361 aaaccctggt cgattctaca tctatacatt tttacaaact tccgttgtta ttggtgcaaa
    24421 tttgatccca ctttacatgg gttaccaata tcaaaaaatc gcccgtttaa aagttttaca
    24481 ttatttaagt cgttttgcct ttatattcct aatagccaca ctagtttgca atatcttttt
    24541 ttacgtacaa gctggcagct tgaatattcg tgattattgg cttatcctaa cacctatcag
    24601 tcagaattac tttacttatg ctgtttcttg tactttgctt ttttgtagta ttccttatac
    24661 agttggattt ttaaataaat tatcgaaaac aacaattaaa tatctactat tatttttaac
    24721 aattggatta gttggctgct ctactctatt taataaagac ccgtttgcat tacagaatgg
    24781 acagagtatt ttatggattt tttatttatt tttaatgggt tatggtttga aagaatggaa
    24841 ctggaaagaa aaggtccgct ataagcttcc tcaagtattt atcagcttga ttgttttatt
    24901 cggtttgatt attttgatga cacaaatttc actcatcatc agaggaaata cagatacagc
    24961 gctgaggttt tctaaaccat attctctctt cgcaatgtat tatactttct cttcgttttt
    25021 atttttagaa atgctatctg ataaattagg tataaaagga agaggaaatt ttcttgctgt
    25081 atttttgatt gtcactcaag ttatatgtaa tttcccttta gtttcttatc gcgtatctac
    25141 atttatgaaa cgtgaatttc caaactcagg gaaggcatgg ctgataaata tcgggcagtt
    25201 ttttatttga tatttattag cagtgtccct cctagttatc tttttatttt atatacaaaa
    25261 aatccgtttc attcatcgtt tcatcgaata cttcgctttt gattcgattg aacaattaac
    25321 tcagcgttgg aaaaaaagaa ttcattggct ttttgttcat aaaagttatt ttttagttgc
    25381 agcatttttt tactgcttta ccttcgtgca gattttcgtt ttagaaccaa aagaaggttg
    25441 gaaagaaact atccaggttg ccttaaaaat atttactcag cgacaagcac ccatgatcct
    25501 gacaatcatc attattatgg gatttttctt cttactattg ttgatttcca atcgtttttg
    25561 gtacgccttg actgctacct tgatcattaa cttactgctc actatttcga cagtaatcaa
    25621 aatggaaatg cgggaagagc ctgtctttcc ttcagattta aaaatgctga ccggattgtc
    25681 agaactatta tcgatggtta gcccagtctt attaatcgtg ggcggattga tccttctcct
    25741 attattgatt actagtatta ttgtacaacg tagactacaa aaacagtatt ccttgaagat
    25801 tcattggaaa cgtcgattta tcagtatcgc tgtattatta ggatgttttt cgggtgtctt
    25861 tttcattaat cacaaaaatt cgccttcttt cttgctgttt aatttattta aagtaaacaa
    25921 gactttcttt aaccagcaag gcgccgtaaa agataacgga ccagtcattc aattcctaaa
    25981 caatatcgac attaaaatca tggaagaacc tgctggctat tcgaaagaaa aaattaatgg
    26041 aataatgaaa aagtacaaca caaaggcaga tgaaatcaac caaacaagaa gtgactggtt
    26101 aaacaatcag acgatcgttc tcaatttaag tgaaagcttc agtgatcctg cacgtgtccc
    26161 taatctgaca gtacctacag accctattcc ttacatcaaa agcattataa cagaaggaac
    26221 aggaggattg atgttatccg tcggttatgg tggcggaaca gctaacatgg aatgggaagg
    26281 actgacaggc ttgagtatca gtaacctgtc tgcttcgtta gttacacctt atacccagct
    26341 agtggagcgt cagaaaataa gtccaaatat taccgatcta ttcgatgaaa aaatagccat
    26401 ccatcctttt acagctgctc tttatcgcag aaaacaagtg tttgataaat ttggttttga
    26461 caggttctat tatttagaca gtccggataa attaacttat acggataaaa tagaacaaag
    26521 cccacgcata tcggatgatt cagcatacaa agaaaccttg aaagctttac aaagcaatac
    26581 ggaaacgtca caattcatcc aattatccac tatgcaaaac catatgcctt atgatgatta
    26641 ctatagtgaa ttgaactaca ctgctgaagg aactgctgtc attcctaata gaagaaaaga
    26701 attacaaact tatatgcaag gccttcacta taccgataca gcagtaaaaa aattcatcca
    26761 agaaatcgac aaaatcgata aaccgattac ctttgttttt tatggtgacc atttacccgc
    26821 aatctatagt gggaatgatt cgaaaaaata tggattagaa cagcaccaga cagattattt
    26881 catttataac aacaagtata gtcgtgaaca agcagctacg ttaaataaag aagttgttgc
    26941 tcctaataac tttggtgcta tggctcttga gcatgcgaat atcaaagtaa ctccttacta
    27001 tgcgttgttg acagaagtat ctaatcatct acctgcatta acaattgatc ctacagaaag
    27061 cttgtctaat cgttttaatg gaaaacaggt ctttgttaca gaggatagtc aaattatcca
    27121 agaaaaaaat ttaactgaga aacaaaaaga aattctagaa gattatcgtc tgatacaata
    27181 tgatcttgtt gcaggtaacc agtacagtgc aacatgggcg acacaaaaaa taaaagacta
    27241 acaaataaac tgagcaaggg gttgtgataa aagacatgtc acaacccctt gtttatctca
    27301 ttaatttgct tgtccataaa cgatttgatt aaagccattt ttcaattcat tattcgctgt
    27361 ctcgttgaag taaagataat tatcgggatt cccctgattt tgttgtgtca ccaatacttt
    27421 tggctgattt tgctgaaacc cgaatagata gacgtgcttt ttcagatatg gttctgtctc
    27481 tgcatcggaa tagacggctg tgatttgata gtctgcttga cctgtcccac tttctgacca
    27541 ttctgcagaa acaggcactc caccaatagc catcttccac tccccgttca aaatagcttg
    27601 agggacttta agaccatata agtccacact catttgattc gtataacttt tgtattgttg
    27661 tcccattgtt tggccccatt tggtcataaa tgcctctaat tgtgcggatt tgtctgcatt
    27721 ccacagtgtt tgttcaactt gttcgctgct ttctgaagag gaagatgaag cctctacaga
    27781 agaacttgaa gtagatgttt gagttgtttc agtcgttttg gaagactctg acgaagtaga
    27841 ggtatctgct gtttctgtac ttgctgcagt tgacttgctc tctgtgctag ttgttgtagt
    27901 agttgcaact gttgctttcg ctgtttcttt tgttgattct tttttcgatt cattcccgtt
    27961 ggtacaacca gctaacaaaa aagctagacc aattgttact aaaatccttt ttttcaatta
    28021 aaaaactcct ttcgattact cccacttctt tattctatca aataagcaat gtgattttat
    28081 tgatttttcg tattttctca tcaatctttc aaatcttcta aaaactgaga agcacccgct
    28141 gatgttgtct gtaatacatc agcgggtgct tcttttattt taatcttcgt gattttccaa
    28201 tttacctcac gctttatatt tgtatttgaa gaaacataga cttttagtca tcatcgtctt
    28261 cctcatccaa atcttgtcgg acagtttttc cttggagtct ttgtacttgg tagatgattt
    28321 ctttattgaa acttaaaagt tcaacaagca attgttgcaa gccatatttt tcttcttttt
    28381 cagctaattt gattgcccgt gtgataaaga cattttggaa atcaaaatct tttgcaaagt
    28441 tttccaacat tgttgaggct ggtaaatatt tgttttgacc atgttcgtaa atttttccat
    28501 aacgcatgac ttcttctaaa gtagacggaa cgatttcatt ttcaatcact aactcatgag
    28561 agattttatt gaaccatctc gcttcttgat cagcatattc tgaaaaaagg gtacttaatt
    28621 cttgcgaaga tgtacccaaa acatacaatt gtgcttggat caatttcatt tcttctaatt
    28681 taaaatttgc taatacatgc cccaccatgg cgccagcagt tggtttatga tggtcgatat
    28741 ctgcttgttt tgtttcttga acaaattttt cttccactat attcattgat ttacgcctct
    28801 tttacttttg ctgattggac ttcataacct aatttttcca caactgcagc taattgttca
    28861 gggtggactt catctggatc aaagtttgct ttgattttgc tcgcattaaa caaaactttc
    28921 gtattgctta ccccaggctg ttgagaaacc gcatgttcga ttttttgcat acagcttgga
    28981 caagctagtg tatctaattg cataataatt tttttcatcg tagttcttcc ttcctttttc
    29041 ttgatagtct tattctagat tacagcttcc ataaaaaaat tgattcttat caagttttac
    29101 gatttttctt ctttgctttc gtgtccaaaa tacaataaac gcatcgcgtt aaagataacg
    29161 atcaagatac tagcttcgtg aacgaacatc ccactagcca tgtaaataaa cccggcaaat
    29221 aagccgttga gtaaaaataa gacgacacca atggcaatca ctacattttc acgggtattt
    29281 aaaacagttt tcttagccaa gcggtaggca tgaaccaacg attcaaaatt agattgcatc
    29341 aagacaacgt cactcgtttc gatagcaaca tccgttcctg atcccatagc aatcccgata
    29401 tcagccgcag ccaaagaggg agaatcattg attccatctc caataaaagc aacgcgcaat
    29461 ccttgttctt ggtatttttt cacaaattga accttttcgt ctggtaataa ttcagcatgg
    29521 acttcatcaa tgcctaacat atcagctaca taatcagcag tcatctgatt atcacctgtc
    29581 agcatcacta actgttttgc tcctgcttgt ttcaacttag ctaattgttc agcgacttct
    29641 ggtcgaatca catctgacac acctatgatt tgagtgattt ttttgtcgat tgctacaata
    29701 atgaccgagc tgcctgtttg ttgtaattga tgtaaatctt gtacttgctt tggtgtgaga
    29761 gaaagtttgt gagcggcaat caatttggtg ttgcccgcac aaactgtatg accatcaatc
    29821 tcagctgtga ttccttgtcc cttgattgtc tgattatctt tgactgctaa ttgttgaaaa
    29881 tcaatgtctt tctcctgagc ataatggatc acggcgcgcc ccagaggatg atcggataac
    29941 gtttctaatc gagccactaa agctaagctg tccccttcat cctctgtata gttcttgata
    30001 gtggatacag cagtagttcc tttggtcaat gtaccagttt tatcaaagac aaaagtatcg
    30061 attttggaaa aagtatccat tgcttcaccg cctttgacta agatgccccg tttcgctcca
    30121 ttaccgattc ctgctacatt cgaaacaggc gcaccaatga ctaaagcgcc aggacaacca
    30181 agaaccaaaa cagtgattgc taaacgaaaa tcacgactaa tcagaccaac aaccaacgca
    30241 ataagtaaaa cggttggagt ataatactga gaaaatcgat caatgaattt ttccgcatgg
    30301 gatttggtat cttgtgcttc ttctactaat tcgataattt tggcaaaggt cgtatcatct
    30361 ccgaccttag ttgcttcaat cgtcacgtag ccatctgcta aaatcgttcc agagaagact
    30421 tcatctccac tgattttgac gacttgtcgt gattcaccag tcactgcggc ttcatccgca
    30481 taccctttcc cgtcaatgac tcgtccgtcc acaggaatag ctccaccagt ttttactaag
    30541 acgatatcgc cttcttccac gtcatcgatt tccacttctt cagtcgttcc atcttcttgg
    30601 acaacactag cagtagatgg ggccatctcg gtcaaagcct tgattgactc ccgcgttttg
    30661 gtcaatgttt tttgttccag atagctacca aacaaaaaca agaatgtaac gatagctgac
    30721 tccttgtatt ctccgatcat aaaggcacca agcacagcaa tagtaacgag taaatcaatt
    30781 gagataactt tgactttcac tgcctgatac gcatggatca taataggtat gaccgcaata
    30841 atagaagcta tggctagagc aatgttatag aacaagacaa aaccagtaaa ttttccgata
    30901 aaaccaaaaa taatcaacac agctgataag gcggtgatat gatttcgatg ctcttggatc
    30961 cacttttgta atttcatctt tctccctctt ttcctttcat tcttacgaga ctaagtatag
    31021 atacttatcc atcataaaaa attgaccact atcaagttta gtcaaaaagg cggttttctt
    31081 ttggtagaat acactacaaa aaatagttag caagaagact cactaaaagt gatcttgcca
    31141 actattgtcg tttctatcga aattttcatt gaatgatttt cctgtacagc atacatcgga
    31201 gatctatttc tttatctaag atttttatct tgctaatttt ttcctttagt ctttcaaatc
    31261 cacgtcgttc atagaattga tacgtacagc cactatctgt aaaaaggaat atttcttttc
    31321 ctttctctct tttttcaaat tctgttagca acttggtacc gatgcctttt gttttcaaat
    31381 caggatttgc agctaaaaaa atgatctctc catctggcga atgcttcttt aaatatgctg
    31441 caagcatttg ttcatttgct gtctcgtaag ctcctttact ttctttagca aatatatttt
    31501 gtaaaaaatc gaatatctta acatatattt gctgtccaaa agaacgatgt ttcttttttt
    31561 ctccttttat ctccgccagc aaaacacctg ccaactcgtc ctctgcatac aaagcaagaa
    31621 tttgagttgc tcgattcaac tccatataca aaaaatatct tccgtaaaga ttcagcagaa
    31681 gttcattttc catatatctc tcaaagttca ttccttcaac agcaaaatag atagcttttt
    31741 tgtaatcttt cttttctaag ggtttaaaag ttagtttcat catttcgcct cctcaacttc
    31801 aatgattgtg ttatacttac attataatag acacagtgtc tactatctat aatcaatcat
    31861 ctataagttt gtcgattaag cgacagcttt gctttgatat gtcggataag gagataggta
    31921 aatgaaaaag acagatagac gcgtcaaaaa aacagaaaaa gctttggctg aaacactatc
    31981 cacattatta gtaaataaaa agattcaagc aatcactata cgcgaactaa cagaaacggc
    32041 agatgtacat cgttcgactt tttatacaca ttataaggat atctatgatc tatatgatca
    32101 attagaaagc aacttttttc gggatctaaa tgaaattctc tcttatgatc cagcccatag
    32161 ctatgaggaa ctatacactc gcttgattga ctatgtttac gcaaatactt ttttatatcg
    32221 cttatttatc ctcaaccatg gtgatatttc gtttcagaag aaagttaaac aagttattga
    32281 agaaaactat ctgaacatat ggctatatga agacaagaaa acgattatca cagaagaaat
    32341 gagattcttt actacgtacc acgtccaagg ttgtctatcc gttctcaatc ggtgggtaga
    32401 aagcaattac aagtactcca aaaaagaatt attagaactc ttacagaaac tgaatgatca
    32461 catagagaaa atcatgcctt gaataaaaac atactaagct tagttaagaa gaaccgtaac
    32521 cttctcgtat aacaactgaa tcttatttag ccaaattgtt tactgaagat tttttgagta
    32581 aaatttctat aattcctact tattgaaaag tagtttgtag tgctgcttct ccctctctaa
    32641 gtgatatatc ttgttgtacc aatcaaattc acctcatcat taataaatta cttttaggct
    32701 aatttattac aacttatagg ttgaagctgt aaccaattta tagtcatatt atatttttat
    32761 catccagttt attgaattct tctcgtttac ctcaattcat tttcaagggg aatcagctca
    32821 tcatctaaca ttttacgcca tgctcgcttc atttcttgtg ggtatttaaa tggtttcgct
    32881 tctggatgtt tggctttata tccttcgtaa tccttactgt tgataattgc tttcatatta
    32941 ggaattggat cttcacctgg catatattgc agattagaag catgtaattt tttctcagaa
    33001 acaaaccact cattggaaaa gacatgaatc aaatcatcac ttacttgtgt aaaatagcga
    33061 cgtttaatga caaagatatc ttcattgcta tctagtgtac cttcatttct ttgtgcttaa
    33121 attcaaagat aattttctca ataaagattt ttgattccag taagattcct ttactccttt
    33181 tacaacatag cgttgtcctt tgttttgaaa catcttcttc ccctctttgc cattaaaaat
    33241 agcccaattc tctataaaga agattaagct tcattattat aatataaagt ttaaatttgg
    33301 tttattactt actttttctt attctctagt aagctaatat tgaaaaaaat gtaatcagat
    33361 cattattttt atatgttgta acttcctgat aagaaaacta attaaaagaa agaggtaact
    33421 acataaacta attaaaaaag ctaacttcat agttggagga taaatggaag aaacttcaat
    33481 gattgtttct ccttgtgttc cttcaatcga cactagtcct attatcaatc tctctttttt
    33541 gtccatttac ttttgcgact gttccccaat aatcaaatac aggaagcgtt attttttgtc
    33601 ctttagattc atgtactgta tagactattt tcgaatccat cacttgataa ggcatagtat
    33661 gccattcctt gccaattctt acttcatgat tctgcaaatg ttctttgtat tgtaatgctt
    33721 ccgtgggtgc atagtcataa agtcctccgg gaaggagtgt cgtgtaacga ataagatttt
    33781 cttgattcat taccaagtga tctctctgag tgacaacttt atgttcatag tttgcttttg
    33841 ttgcagcaga tacagccaac agtaataaaa caattgccgt tatattaact acttttcttt
    33901 gcttagctga tgtgctcttc atgaaaacga acgataaaat caacgagcca aacagtactt
    33961 gaaagcctaa gattcgccaa ggaaattgaa tcatttgaat gggtgtattt tgtaataaag
    34021 accatggaaa aaatttggtg gataagagcc aagagactac cgcactaagg taaatcactt
    34081 tttctttgcg attaaatcct ttaaaaaaga agagtggaca gatcaaagaa ataataccaa
    34141 tgattcctac cgtaaaggtt gtcaggtcat tattcatcgc accaatcaaa ctatccgaga
    34201 catttaatgc ttggtgttgt aaggtcgttt tatctggctc attaatcgat tgaaccatga
    34261 tttgtcggaa catcggatac caaaagtatg ctgttaaaag aatcgtcatt cctactgcct
    34321 ttgacaactc cacaatcaac cggatgcttt ctttttttct tgtttcactt actacaaagc
    34381 cgcctaagac tagtaagcca acggctatta cactcataaa agtggaaaga acatgtgtat
    34441 aaatgagtaa gctcatactg actgctaatg gaattgcaca tggctttttc tcataaatca
    34501 gtgcgtaagc ataatacata actatcggta taaccgtaag tgcaatcgct tcagcaatag
    34561 cagaacgatg ataaatatca atcgtacggt acccataaaa tgtatataaa cagctaaata
    34621 taaaagccgc atcgattctt tttaaaaatt ttagtccata atgaaacatc agcatgatag
    34681 tacaaacatt caatagccaa acatataaga tataactaac gattaaatta ttacttatcc
    34741 aataaaaaat gactgccgga aaaaaggtca agtagggata aaaataattc actccgctcc
    34801 cataatgatt aaacgtcgta aaattgattg gaccagaaaa aattgaagat aaccctttga
    34861 ctctagcaaa atggaaggca atatcccccg atgtagaaat agtatgaaca tttctaaaat
    34921 aattgattga ataaagtcca gcaataattg ctaggagaat ggacaatatt atttttttct
    34981 ttacttgcat tacttaatta tatactcctt tcatggattg ctttatctag tataacttaa
    35041 attttaaata aatcgttatt tatttttact ctcacttaaa aaagcactaa aattataaga
    35101 gagggaggta atggtctgaa caccaagaat tgttccaaca tcgaattgct taactaatga
    35161 gttcaaagct taattatgtg gctcttatat ttaccgagcg tggattgcga tctctttagc
    35221 gaaacaaaca tctttcataa aaaaaacagc catatccgct ttcttggata tgactattta
    35281 ctactacttt actatctttc attcttcttg tctcacttat gataaggcgc tgcgtatcat
    35341 gagtgaatga tattaggaaa acttcataaa ataaatcttt tatacatttc cacttctaga
    35401 tggtcattca tgttagaaga atcaaactat ttttttactg tcgtaataat attaaaacac
    35461 tagaacaaat caattaaatt ccataaaata taaataatag acctagcatc atctggtata
    35521 aaatggatgc taggtccgtt atatttactc aaaattgatt ctacgaatag atcttcttat
    35581 catttctatt ttagtagtca tgcgttgttg ttcttctaaa ggtggtaatg gtataagtag
    35641 tttagataaa tttgtaccat ttacatttgg ttgcccagtt ccgtaagaaa ttttttctaa
    35701 taacttccaa tataatggcg agtctaagaa gcagtctacg tattctgata ataatttctc
    35761 aactaaccta attcgaatca agtaagaagc aaaaacagat tcctcactta tttccttaac
    35821 caagaaactt tttcccatag ttcctcctgt ccttgctatc aaaatgtcat tttcttccaa
    35881 tcttaaatca actagtttag aattactaat atcacagtaa ggcacactgg accaatttac
    35941 acgtccctct tgtatatctg tgattctcaa aaacttaaca ttgccttgtt ttttcgccga
    36001 atctgtatag ccatattgaa tcttagtaga aatacttcct aaataaaccc atagtcgaac
    36061 atcgctagat gatttttctt gtcgttcttt atgaacaaca gtgatcatct tgagttgtcc
    36121 gactattttt tagttgcagc aatctttttg ggacgcttat ttgcatgaag caacgttgct
    36181 aagtctagta ataaaacatt ggctaacaca ggattggctt tctcgcgata cgctgcggcg
    36241 ccactttcaa acaatactgc tagacgatct tctacaacaa cgatttgttc caaatacggt
    36301 ggaaatttga ttcgttttag gatccggcta ggagagtccc gctcggacac taagagttcc
    36361 gaggtataag gaccataaga acgactggct aagagataat ggccataaaa agccacacct
    36421 tgaatcttat cagatgtttt tctgctcatt gtcacggttg gttggatttt gtctttgcgg
    36481 cgaattgctg ttttcatttc agctatcgtc ggcaatagat agaaagtcaa ctgtccttca
    36541 ccttttagtg tgaagttgcc agataccagt tgatcattgt tttgagtcaa atacgatgct
    36601 tgtggaatct ctgagagatc agttgtcagc caataagcga ttggctgtcc cgtctcttca
    36661 gatgtatctg ctaagatatc gatcaaacgt aaagcggcta ctcgtccagt agcagatcct
    36721 gtgattgtca accataaaag ttcccgtttt gtatcatata ctaagccccc tacatgtggc
    36781 cgtttaggta aaatcagcag tttgatcggc tgtcctgttt ctctatccag tacatgaata
    36841 atagaatgat gctgatggtc atagcagtag gcagaaataa acacaaaatc ttgagaaaaa
    36901 gccaaccctt gcggcgtcat ccattgacaa acctcatttt cgccggtttc cggcaccacc
    36961 attcttgttt ctgttaatcc aggaatgtta tattgttttg cttcagcctt ttcccaagct
    37021 gcttgaacaa taggataccg ttttaacttt ttttcaatat cctgatgtat ccgttgctgc
    37081 ttccaatgct taaactttag gacatagtaa acaagaaggc caatgaccag caacactaaa
    37141 atcattattg ctacccgctg tgtcattttt ttgcctcctt gttctattcg atcaatcgtc
    37201 catataccgc tattaattac cgtaactaca tgtttctttt actagcgttt ctttatagga
    37261 tctttcataa tctcatttat agtaacatta agaatccctt attctactat agtcacttcc
    37321 acatcaatat tgcctcttgt tgctttagag tatgggcaaa atgcgtgggc ttcttctgcg
    37381 attttctctg cttggtcctt ctcaagtcct ggcaaagaaa cttctaaaac gaccccgatt
    37441 tggtagcccc catcttctaa ttgaaataaa gatacccgtg ctttgacttc agaatcagac
    37501 tcaagattat tttcttccag catgtgttga accgcgccgt tgaagcaaga tgcgtatcca
    37561 gccgcaaaaa gttgttccgg atttgtccct tcactatgga ttcctgggcg gtcgatcttc
    37621 atgtgcatac ttccgtttgg tgcctcaact tcgccatcac ggcctccacg attgatcatt
    37681 gctgtttcgt acattttttt cattgagatc atcctttctt tcgtcgttct atattaagtg
    37741 taacgagtat tttttaatct gacaaaggta atgcttgcat tcggcgacat cagttagaca
    37801 acatcgatga tcagtgatag aagcagttta tctatacaaa aaaagaaggg ttctatgata
    37861 tttggtgtcc ttactcgcct tgagtaacaa caccaaatat catagaacca cttaatcagc
    37921 ttcacactgc tttttctact ttcgtagttg aaaaaaactt aaacaaatca tcggcaaata
    37981 gttaaagaaa aaacttaatc gttgaaagaa gcccaattgc ttaaagaagg caaactgact
    38041 aatcgacgga atccgtgcta atccgtaaag acctgcaatt agtaaactaa taactgttaa
    38101 aacaagataa aaatggctaa gcgtaccttg accactttga cgataaagca gaacaagtaa
    38161 caatgggaaa agtagaaagc ctgcatagcc tagcccagaa cctatattat ggatccaagt
    38221 cgatagatta caagagcttt cattcgtatt gatactgaat aagccagtaa aaatacaatc
    38281 acctatccca tataaaccaa ctgttgcaac aacaagtatt gcaagtgttt ttgatgttcc
    38341 aacaaatagg tgatacaacg caggcaacga aagaacaaaa aaaagtccag atacaactga
    38401 ccagaccaag aaagcacgtc tgactggact atctacgtca ccaaacacac taattacggt
    38461 agtcatttga ttcagtttgg gataaaaaag gccaaggata tacggtgtcg ataaatcact
    38521 catcacacct aataataaaa aataaaaacc atatttttgc aaaaaaatca ttttcttccc
    38581 ctacttttta gtgattattc gatacatcca acaatcaaat tttttctcat tttaacacat
    38641 atttttattc tagttgtctt ttgaacaatc aataacataa aggtgagttt cttctacatt
    38701 ttaaaaaatc gaatggcact gaagagttca gttgtcagca tcaatctcct actcaaaaag
    38761 gcaaaaaaac gtaagtcaga tgttgttgac atctcactta cgcatccatt gattgaaaat
    38821 ttcttagtaa aatctactag cctaaaaatt tgaagacttc ttctcgatca tacagtttaa
    38881 atactttctc gcataaaaat tgagtaatta gtgccgcaaa aacagggatg actaaccaac
    38941 aaagaacaac aagaaagcca tttagccctg catctaaaga agccaacgga ccaacaagtc
    39001 cgaccacgcc gaaaccagca gaagcaggcg tacctgaaat agaaaataga gcgacaggaa
    39061 tagctgtaat cactgcacta actaaacaag gaataagaat cacaggtttt ctaaatagat
    39121 taggcatcat catcttcata gcgcctaaag caatcgcaat tgtcacacct gacttattaa
    39181 ccttccaaga gttcactact aaaacaaaag tggttgcagc tacgcccatc gcagccgcac
    39241 cagcagaaac accatctagt tggattgcca ttccgattgc tactgttgaa ataggagaga
    39301 taatcaaaaa ggcaaaggaa caaccaatca agatagacat taacagcggt tgtaaggtag
    39361 tgaagttatt aatcaatgat ccaattgcag aagtaattgc tgttacaaaa gggaataaat
    39421 aaaaaccaag taaacctgca cctacaccta caacaatcgg tgttaacaca atcgacactg
    39481 acccaaaact atttccaatc agtaaaatca gcccgacagc aatcgatgct gtcagcatcg
    39541 tattaataat atcgcctgta ccagcaccaa cgtaaacacc cgtttcaccc aaatcaggtg
    39601 tgaatttgac tacaccagaa ccaacaaaag aagctccggc tacaaccatc attctcatcg
    39661 gatcaaatcc aaattgtaag gcaatcaaac caccaatgat caaaggtgtt gctagttgaa
    39721 aaataactgc taactgtgcg atcgttaccg caaagggata cgcactaaaa tattttaaaa
    39781 tggcacctaa tactgcatta ggaatcaatc caataatgat tcctgttgct gtaccagcga
    39841 ggactttatt cagaaaaatt ttcggtgata ttttttgtga agtttccatt caatctctcc
    39901 tttattttac gttcaaacta tcttcttttg catggacaag ctcagtcaag aaatcacaat
    39961 aaggcgtcgc aacaccatat ttcttccctt ttctagaaat tgcgccattg atataatcga
    40021 tttctgttaa tcgatggttc ttaatcaagt cttgatacat tgaaggataa tgtaacccaa
    40081 ttgtttccgg gtcaaaacaa gattcacaat gtgcaatgac ttctgggaca tctagttcaa
    40141 tcttctctac tgctgctact ttggcaaact cattgacaat cgtcgccacc attttatgag
    40201 cagtcgatgt ttttcctagc tcagccatat taacgtctaa gattgtgcat agtccgttca
    40261 ttgttccgtt cacacatgct ttgcgataaa tagaatagtg aatattgtca gaaaaatgag
    40321 catttaatcc tgattcagac aatttgtctg ctaatttttt tgcagctgct tcttttccat
    40381 cacctaaatt ttgtaattct actgaaccac ttccaaataa tttgacctga ccaggacctt
    40441 caagacctgc cgtccacatg gtatttccaa tatagatatt ttccattggt acaaatttct
    40501 caataatatc ttcatgcccg ataccattta atagacataa gacctctgta tcttttttga
    40561 ttaatgattg gatatcctgc agcatttttt ccagctgcat cgctttggta aatagaataa
    40621 tcagatcaac ttgatcttct ttttctactt cggattgaag aacgattggt agttttgctt
    40681 ctacctcttt accattaaaa tttgcttgta atccatgttc tttgatttgt tggacatgtt
    40741 ctgcccagcc atcgattaat aatacttcgt tcccactttg gtgaagcatc aacccaaagc
    40801 gacttcccat tgcgccagcg cctgcaattg ctattttcat tgattcttcc tcctttttct
    40861 ttctattata ctcatttgag aatcttttca caacctcgtt ttgttattct tctcttattt
    40921 atcatgttta tatccaccat tcaagttatt ttatcagcaa atcaataaaa actggaatag
    40981 aatcccaatt tcatcgtaag agaaactaga ctagcaataa gaaaatagaa acattcatag
    41041 aaaaaatctc actcctctga ttataaaaga gggggcatta acctcgtgta tactaaaacc
    41101 ttccattcat tcgatggatg attttctttt tgtgaaaaaa tattcattgt agtacgatgt
    41161 tgcgcaacac agacggatgg tattgcttat tatttattaa aatcgaacaa tacacttatt
    41221 taaaaacttc tttcccagat tttcttccat ttttacagtt ataactagta ttatcatgaa
    41281 aaatagcctt ttattgtcta tgggaataat aatttaaaat tggtact
//
LOCUS       DARBET010000018        41155 bp    DNA     linear   BCT 07-NOV-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4027
            SAMEA114569841-rid20112183.denovo.018, whole genome shotgun
            sequence.
ACCESSION   DARBET010000018 DARBET010000000
VERSION     DARBET010000018.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA114569841
            Sequence Read Archive: ERR12203666
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 41155)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 41155)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001972618.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001972618.1
            Genome Coverage       :: 132x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/07/2023 13:18:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,041
            CDSs (total)                      :: 2,975
            Genes (coding)                    :: 2,908
            CDSs (with protein)               :: 2,908
            Genes (RNA)                       :: 66
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 67
            CDSs (without protein)            :: 67
            Pseudo Genes (ambiguous residues) :: 0 of 67
            Pseudo Genes (frameshifted)       :: 26 of 67
            Pseudo Genes (incomplete)         :: 28 of 67
            Pseudo Genes (internal stop)      :: 20 of 67
            Pseudo Genes (multiple problems)  :: 6 of 67
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..41155
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA114569841-rid20112183.denovo.018"
                     /isolate="VRE4027"
                     /db_xref="taxon:1352"
                     /country="Denmark"
     gene            complement(441..1223)
                     /locus_tag="SCJ86_001047"
     CDS             complement(441..1223)
                     /locus_tag="SCJ86_001047"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317770.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEG1168625.1"
                     /translation="MENSSNSAEVNVSTSTSEVVKCSQETTSLPEELLGDWQINNKDA
                     GIEISDSKAVFRIGRSQVESKDYYVDVNNKMLLHVYFSFEYDSVKYIEVSIERTGYSE
                     FISKSVGILTMHTKDGQYISLGVKRVSGENNETEAVTEQTMDERIDSSKSNETEVDTG
                     KTIDERIDSFIETYTAIPPTWKLVNEDKGITINTDTEISTDAQSLSDLLANLTGISNA
                     VTEQVGKDIPLRLLENGKKDYSVVFKNGQVTDYNEVFKKVIE"
     gene            complement(1288..2124)
                     /locus_tag="SCJ86_001048"
     CDS             complement(1288..2124)
                     /locus_tag="SCJ86_001048"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298387.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PRD domain-containing protein"
                     /protein_id="HEG1168626.1"
                     /translation="MRVIKTLNNSLILCLDKQGKEVILMGKGIGFQLKKGDLVPADKI
                     QKVFKLDSLSDALKKDYIKLFEAAPDDLIICVKRIIDYANAKFDFKLNKSLFFTLVDH
                     LKHAIERSQKGIVFQNRLLIEIQKYYPKEFEIGCYAIQLINKKMNVQLPEEEAGNIAF
                     HIVNAQSEQANMEETMLSVRALKDILAMLGYLFPEKKVDKDSMLYLRFVTHLQFFIAR
                     MIKKEELPEKDDFLLASVKQRFPKEYRAALKIKEYVQEEMGASANQDELLYLTLHISR
                     VY"
     gene            complement(2142..3551)
                     /locus_tag="SCJ86_001049"
     CDS             complement(2142..3551)
                     /locus_tag="SCJ86_001049"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010757186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 1 protein"
                     /protein_id="HEG1168627.1"
                     /translation="MQYKQLSAFPEKFLWGASISAYQAEGAYQEDGKQPSIIDKYQHT
                     EGVANFEVASDFYHHYKEDIQLFKELGLKAFRFSIAWTRIMDIENNKTNQKGISFYHQ
                     VIDECLNQGIEPIVTMYHFDLPYFLEEQGGWLNRATIDYFVDYVQVLLTEYGDKVNYW
                     LTINEQNTMILHPGAIGLPPSGELPSKKELFQINHHVLLAQARVIHLYHQLNLKGKIG
                     PAINLTAMYQATSSPEDAIAAHNWETLRGWSFLDVAVRGKYNYLFENYLNDRGLYPKI
                     EAEDQAILSSGKPDFIAINYYSTATIAASKNDGSDVSARAGDQQIMLGEEGVYRAAEN
                     PYVDKTPYGWVVDPTGLRLTLRKLYDRYDLPILITENGYGAPDIVEEDRRINDQDRID
                     YLAKHILAIQAALTDGVDVFGYLPWSAIDVVSTHQGFNKRYGFIYVDRTDENLKELKR
                     IKKESFFWYQKVIARNGLA"
     gene            complement(3565..5388)
                     /locus_tag="SCJ86_001050"
     CDS             complement(3565..5388)
                     /locus_tag="SCJ86_001050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337875.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucose transporter subunit IIA"
                     /protein_id="HEG1168628.1"
                     /translation="MDYREVGRNVLQQVGGKENVVSLTHCATRLRFELKDKRKADTKA
                     LEKTLGVISVVDSGGQYQVVIGNEVQTAFKEIQKEVGTSIKKGPSSDNQESIISRLIS
                     VISTTFTPMIPAITGAGMVKAILAVLTLTGVLSDSSQTYIILNTIADAAFYFMPVLLA
                     YGAAIKFDVNPILAITVAGILLHPNISQLFSTGDAVSFLGIPVLAADYAGSVLPIIFT
                     VWIMSYVEPFADRISPSFIKFFTKPMLVFLIMGPLALVVIGPFGTLLNDLIAMGAEWI
                     NGKAAWLIPFLMGGLQPFLVVTGTAWAMTPIATMQLSSTGSEMINGPGMLASNIAQSG
                     ATFAVAAKTKNKELKQLAVSTGITALMGITEPSLYGVTLKLKKPLVASMIGGAIAGLY
                     AGLSGLVRYAFVSPGLAALPAFIGENPMNIVHAIITCVIAFVATFIIAYLIGFDDTSD
                     ESEQTAIAEAVIVENPVKGTVCPISEVNDAVFSKGLLGKGVAIHPEENSIYSPVEGEV
                     ITILESKHAIGLRSKEGIELLIHVGIDTVNLKGEPFEVFVKAGDQVSAGQLLINADFA
                     AIRNAGYDDTVIVVVTNSENYAGISEASGSLKEHEPLMQIS"
     gene            complement(6231..6557)
                     /locus_tag="SCJ86_001051"
     CDS             complement(6231..6557)
                     /locus_tag="SCJ86_001051"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334408.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEG1168629.1"
                     /translation="MNKVNMNKKFVIIGIVVLAIIGIIFSKMTGTSGLEKSSKPLVTQ
                     ILQEQYNLNRSCDDVTITHENGDNTYRAKAILDNGSAININIEYYPKKDRIYVEIPYA
                     EVLMLN"
     gene            complement(6603..6965)
                     /locus_tag="SCJ86_001052"
     CDS             complement(6603..6965)
                     /locus_tag="SCJ86_001052"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010717431.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ribbon domain-containing protein"
                     /protein_id="HEG1168630.1"
                     /translation="MYCRKCGKEITDESKYCQFCGALQETDETTGTDTVKKSTEQTIN
                     EMKEQIVHGQETGKLYLIIGWISMVVSLAFIPVVFGAVAVIMGYLYREKDEKMGTILM
                     IAGVAGAIFGVLLGMSMY"
     gene            complement(7002..7217)
                     /locus_tag="SCJ86_001053"
     CDS             complement(7002..7217)
                     /locus_tag="SCJ86_001053"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HEG1168631.1"
                     /translation="MKKVSNLSRYRKEKGLSQTELAKKMNVTQQCVSSWQTGRTIPKP
                     YQMKMLSEILSVPMNELFSDVFNKVNS"
     gene            7344..7679
                     /locus_tag="SCJ86_001054"
     CDS             7344..7679
                     /locus_tag="SCJ86_001054"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328543.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HEG1168632.1"
                     /translation="MKQKQYAKVIREKRKALGWTQKELAEKIFSTQQAVARWENSVTE
                     PNLDSLTALSRALGTPVSHFLDNVVVDYEEEFLALYRSLSTEDAVRTIDYMKLLKRQE
                     NERNQLLKD"
     gene            complement(7755..9752)
                     /locus_tag="SCJ86_001055"
     CDS             complement(7755..9752)
                     /locus_tag="SCJ86_001055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331149.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEG1168633.1"
                     /translation="METSMINIPIYVDQEKLLEKPVTKKEKTDVRDRCLDNYREVTRD
                     EFVEILNSSQSFIPSKTKNKGNDKESFVETRVIILDVDNTVKDEKGKVVDLSKDDSRY
                     LSVEKVLSINLVHNSAFAIQKSIRYSENLEKYKIVFLLKEAITDYGKMVAVYEYLKKN
                     IPGCDDNVNASNRMFFGGYKSDAVIIINEDNMLDITELPIDFTMTCSKNCQSDSGREY
                     ELVNETDFVKLIKNDDKEEMKQWFKDCLFDSSDMSFNEIYERLLTIDMNKLLKSSKNL
                     RCLFHNDHNPSASIFTSKTGHSIFYCHSSNCDISENFIGVVMLILKKDSRVETFNWLL
                     ENLDLYPTHFRKLKEESKVLFDTLESMKDKLFRTIRNYLEEMRRVYDVLLSDVNFFDD
                     SKESVTCILSGEQLAKKMSSYYGKTYDIDKCNKLLSLMTFIGLIKKLDDEDVPLKMLE
                     YLNRLKEEKALGNFNHKRSNVYRLDVFNATSVIQKLNEDILPLLEKIHFTYQYFSYEW
                     VKICFNEKEAKRVFPQNSRSELSKEKQLILDEAHEVLQGLSWQGTYLMTEKELINTVC
                     ERLRNVGDNSVKNALIKNRGYFVKQGFTLERASKDIKKLYNVKASSSFLIYLAKGAGI
                     DETIKELILSGAGIVPLYQFTSVTRDDGRRVRKRKDPIVLN"
     gene            complement(9850..10125)
                     /locus_tag="SCJ86_001056"
     CDS             complement(9850..10125)
                     /locus_tag="SCJ86_001056"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HEG1168634.1"
                     /translation="MSLQIIDSIVSIQVEQQVKEIITVLKPMLEDIISGVTLEYMDYQ
                     QASEYLGVSVGTIRKYVSQYGLPVIKIDTVIRFKKSDIDSFMERYKG"
     gene            10357..11091
                     /locus_tag="SCJ86_001057"
     CDS             10357..11091
                     /locus_tag="SCJ86_001057"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301918.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HEG1168635.1"
                     /translation="MIKRPPINYLERKKILGTKIKAIRKSKKLTQPAFGLMINNGQLI
                     DKKTIYEWEKGTYLPIPERLSRIADLGNMSIEELVCGNVEEYILGIILYRDSIVLDGI
                     TFPDKNLFQHLRQQFPPVHSNLDTWLDRYSKLEPEMQEFIANKTCNKVKNEKISLFNI
                     LKIEELFINAIVEEFDNNILFLTSSIEELLERMVDEWLPIQLKDMSYPEEAVREITDN
                     INKLEQTISSIGKKYTKKKMKGGDTI"
     gene            11130..12272
                     /locus_tag="SCJ86_001058"
     CDS             11130..12272
                     /locus_tag="SCJ86_001058"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301919.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HEG1168636.1"
                     /translation="MAKISKYKKKNGQVAWMYKGHIATDPRTGEKINTTRRGFSSKQE
                     AQKDYDEYRHQILYGVKKKAPDMTFEDLYNEWITHQRTSVKASTIAISVRYANNQILP
                     AFGKLKLSNISVPYCQKVVDEWHSKYESYDYMRKQTAQILRYGVAMQYIDNNPMEKTL
                     LPRKKEYEKNRKFYSKEELNNLLDAFKDFGNMKQYAFFRLLAYTGMRKSEVLALQWKD
                     IDTFNKELHVNKTLAVDEFGKVIIQSPKTRASRRVISLDTETLSILNNWKLQQKEEYL
                     KLGYNTSSKEQHVFTTVKNTLYIPNTVNDWLRYILKKYNLPRITPHGFRHTHASLLLE
                     AGESVKVVQQRLGHENSKVTLDIYAHITNNAPKKTGQDFADMMAHQ"
     gene            complement(12367..13758)
                     /gene="sufB"
                     /locus_tag="SCJ86_001059"
     CDS             complement(12367..13758)
                     /gene="sufB"
                     /locus_tag="SCJ86_001059"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623216.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly protein SufB"
                     /protein_id="HEG1168637.1"
                     /translation="MGVPELEEYKFGFHDDVEPVFSTGEGLTEEVIREMSRIKGEPEW
                     MLEFRLKSLETFNKMPMQTWGPDLSDIDFDAIKYYQKPSDKPARDWEDVPEKIKDTFE
                     KIGIPEAERAYLAGASAQYESEVVYHNMKDEFEKLGIVFTDTDSALKEYPELFKEYFS
                     KLVPPTDNKLAALNSAVWSGGTFIYVPKGVKVDVPLQTYFRINAENTGQFERTLIIVD
                     EGASIHYVEGCTAPTYSSNSLHAAIVEIFTRKDAYCRYTTIQNWSDNVYNLVTKRAKA
                     YEGATVEWIDGNLGAKTTMKYPSVYLDGKGARGTMLSIAFAGANQIQDTGAKMIHNAP
                     NTSSSIVSKSISKDGGEVNYRGQVTFAKNSAGSISHIECDTIIMDEWSKSDTIPFNEI
                     HNSQVSLEHEAKVSKISEEQLYYLMSRGLTESEATEMIVMGFVEPFTKELPMEYAVEL
                     NRLISYEMEGSVG"
     gene            complement(13763..14233)
                     /locus_tag="SCJ86_001060"
     CDS             complement(13763..14233)
                     /locus_tag="SCJ86_001060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016632039.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SUF system NifU family Fe-S cluster assembly
                     protein"
                     /protein_id="HEG1168638.1"
                     /translation="MGLSKLDNLYRQVILDHSSHPHHRGSLTDSTSKIELNNPTCGDV
                     IELQVDVKDGIIKNIAFDGSGCSISTASASMMTDAVIGKSIEEAEHLTVDFLQLVQGK
                     EVKDPDQLGDAAMLGGVAKFPARIKCATLAWKALGKAMENDGTAAFHHYHKEEE"
     gene            complement(14220..15455)
                     /locus_tag="SCJ86_001061"
     CDS             complement(14220..15455)
                     /locus_tag="SCJ86_001061"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016632038.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine desulfurase"
                     /protein_id="HEG1168639.1"
                     /translation="MIDAKKLRNDFPILFQDVNDEPLVYLDNAATTQKPKQVLQALAY
                     YYEHDNANVHRGVHTLAERATHDYEQAREKVRAFIHAKETAEVLFTRGTTTSLNWVAR
                     TFGDRYIKAGDEIVLSYMEHHSNVIPWQQLAKRTGAVLKYIELTEEGFLDMEDAAKKI
                     TDKTKIVSIAHVSNVLGVFNPVQELAELVHENGGVIVVDGAQSAPHMAIDVQALDCDF
                     FAFSGHKMCGPTGIGVLYGKRKWLEEMEPVEFGGEMIDFVELYDSTWKELPWKFEAGT
                     PNIAGAIALGHAIDYLQKLGMDNIHRYEEELAAYVLPKLQAIDGLTVYGPQDPNFRTG
                     VVAFNLDGLHPHDVATALDMEGVAVRAGHHCAQPLLKYLEVSATARASFYFYNTTQDA
                     DRLVEAIMATKEFFQHGTI"
     gene            complement(15452..16738)
                     /gene="sufD"
                     /locus_tag="SCJ86_001062"
     CDS             complement(15452..16738)
                     /gene="sufD"
                     /locus_tag="SCJ86_001062"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016171125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly protein SufD"
                     /protein_id="HEG1168640.1"
                     /translation="MKEKNWLDYLDAVNDFSLSKGEPDWMRTFRQDALAKADELPLPH
                     IDRVKFHRWSLFDVKETQTISETGTIPAFDAMKDNPVLVQQGSWTIFEQLPVELAEKG
                     VIFTDLFTAMIEYPELVQEYYMKKAVNMNEDQLTALHVAFMNSGIFLYVPKNVVIDEP
                     LESLFIQDGASDEHFFKHVLIVADEHSEFSYLERFQTTKEQVAKSSGNIIVEVIAKAG
                     SKIKYSAVDQLGENITSYMNRRGHILRDASVDWAIGVMNDGHVIADFDSDLAGEGAHA
                     EVKIVAISSGRQIQGIDTRVTNKAPHTIGHILQHGVIREKGTLTFNGIGHILKGAKGA
                     DAQQESRVLMLSDKARGDANPILLIDENEVTAGHAASVGRVDPEEMYYLMSRGLHKEE
                     AERLVIRGFLGSVLTAIPVEQVRKELVEVIEGKLNG"
     gene            complement(16751..17524)
                     /gene="sufC"
                     /locus_tag="SCJ86_001063"
     CDS             complement(16751..17524)
                     /gene="sufC"
                     /locus_tag="SCJ86_001063"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005879209.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly ATPase SufC"
                     /protein_id="HEG1168641.1"
                     /translation="MSVLEIKNLHVSIEEKEILKGVNLTMKTGEIHAIMGPNGTGKST
                     LSAAIMGNPNYEVTEGEILFDGKNVLDLEVDERARLGLFLAMQYPSEIPGITNAEFMR
                     AAINAKRDENDKISVMAFLKKLDEKMALLNMPEEMAERYLNEGFSGGEKKRNEILQLL
                     MLEPTFAILDEIDSGLDIDALKVVAKGVNEMRGDNFGALIITHYQRLLNYITPDVVHI
                     MMEGRVVMTGNADLAKRLEAEGYAGISKELGIDYKEEEA"
     gene            complement(17721..18569)
                     /locus_tag="SCJ86_001064"
     CDS             complement(17721..18569)
                     /locus_tag="SCJ86_001064"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287678.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MetQ/NlpA family ABC transporter
                     substrate-binding protein"
                     /protein_id="HEG1168642.1"
                     /translation="MKKKIFGFAAALLLTVGLAACGNNSDSKDSANKADDTTLKVGAS
                     PTPHAEILEHVKPLLKDEGIDLEIVKFDDYVLPNQSLEEGDIDANYFQHIPYLNKEIK
                     EKGYDFVNAGAVHIEPMGLYSKKVKDVSELKDGATIITSNSESDWGRIITILQDAGLV
                     KVKDGVDLETATFDDIEENPKNLKFNHTIDPSLLASTYQNDEGDLVAINANFAYGAGL
                     NPLEDAVLLEKDNSPYVNIIATRKGDEDSDKIKKLIEVLHRKEVQEWITDKWGGSVKP
                     VKADAK"
     gene            complement(18593..19279)
                     /locus_tag="SCJ86_001065"
     CDS             complement(18593..19279)
                     /locus_tag="SCJ86_001065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEG1168643.1"
                     /translation="MDKSFSETYLDFKQINPETMNQAILDTLYMTLISMVLVTIIGLV
                     LGLILYSIGRKNKPYARIIYGIVSIISNIFRSTPFMILMVLIIPFTKAIVGTMLGAQA
                     AIPALVLSAAPFYARLVEIAFREVSPGVLEAADAMGASYWEIIWKVLIPESIPALISG
                     LTVTTISMIGFTAMAGAIGAGGLGGLAWQEGYQRGNLTVTFVATLIILLIVFIVQGIG
                     DFLTKKTDKR"
     gene            complement(19272..20306)
                     /locus_tag="SCJ86_001066"
     CDS             complement(19272..20306)
                     /locus_tag="SCJ86_001066"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738389.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HEG1168644.1"
                     /translation="MSLIQLTNVKKTFNGKNGNIQAVNDVSLNVEKGDIYGIVGYSGA
                     GKSTLVRLLNGLELPTSGEVIVNDQDITKLKNKELRTFRKKIGMIFQHFNLLWSRTVL
                     ENIQLPLELAGVPKSKRKERAEELLRLVGLEGRGQAYPSQLSGGQKQRVGIARALAND
                     PEILLCDEATSALDPQTTEEVLDLLLAINKKLNLTIVLITHEMNVIRKICNKVAVMEL
                     GKVVEEGDVLTVFRQPKKEVTKRFVQQDIEPEADSTELLAELIKENPSGRLATLTFSE
                     RNANEPVISQAIRRYNIDVNVVYGKIKQVKEGSFGSLTVLMTGETKELDQAEVFIKEQ
                     GVGIEVIHRG"
     misc_binding    complement(20369..20625)
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00230"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="T-box leader; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="tRNA"
                     /db_xref="RFAM:RF00230"
     gene            complement(20701..21039)
                     /locus_tag="SCJ86_001067"
     CDS             complement(20701..21039)
                     /locus_tag="SCJ86_001067"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine cleavage system protein H"
                     /protein_id="HEG1168645.1"
                     /translation="MAGKHLKKKDNLWILFNGKEYCVGLTNEAQEELGDITFANLPKA
                     GQSLKKDEPLIEVEAEKAVNEFASPLTGTVSSVNDKIGEDINILNDSDEMNAWILSFK
                     DVDPKEFDEL"
     gene            complement(21041..21397)
                     /locus_tag="SCJ86_001068"
     CDS             complement(21041..21397)
                     /locus_tag="SCJ86_001068"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arsenate reductase family protein"
                     /protein_id="HEG1168646.1"
                     /translation="MYTFYWYPKCSTCKKAKAWLDQHGVSYQTVDMIKEVPKASTIET
                     WLETSQVPIRRLFNTSGMKYRELGLKDQVGEFTASKASQVLSTDGMLIKRPLILREDQ
                     LVAIGFNEKIYEGELI"
     gene            complement(21403..22590)
                     /locus_tag="SCJ86_001069"
     CDS             complement(21403..22590)
                     /locus_tag="SCJ86_001069"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344548.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FtsW/RodA/SpoVE family cell cycle protein"
                     /protein_id="HEG1168647.1"
                     /translation="MEKKLKASNDNRIDYGVILPVFLLCLIGLLSLYVALSHDPNNPA
                     VLKGVGMQVVWYLVGAAAIVVIMHLNSKWIWKLTPYLYGLGLVVMGLLLKFYDQNLAA
                     STGSKNWFRFGAFTFQPAELMKIAYILMMALIVTKHNTQVKERTMKSDFWLIGKLLIV
                     TIPVLALIMAQDDFGTMLVFLAIFGGIFLMSGISWRIIAPVVILAVVVGAGTIFLVTT
                     EGGRDLLYKVGFKSYQFARIDSWLDPFHDTSGMSYQPAQGLLAIGTGGLFGKGFNVSN
                     IYVPVRESDMIFTVIGENFGFIGGAFVIFLYFILIYRMIRVCFDTNNEFYAYIASGLI
                     MMLLFHVFENIGANIGLLPLTGIPLPFISQGGSSILGNMIGIGLILSMRYQNEAPARS
                     SRR"
     gene            complement(22781..23626)
                     /locus_tag="SCJ86_001070"
     CDS             complement(22781..23626)
                     /locus_tag="SCJ86_001070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327411.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HEG1168648.1"
                     /translation="MFKLLTTFRAVYETRNFSRAAEVLFLSQPAVSNQIKQLERELNI
                     DLFQRNGRKEMLPTKQADLLYERTLVLLEEWEETQQKLLNEQNEEEVCRIVASHTFAV
                     YLLPQLMKHLIKRFPKVHFEIEITNSHESLEQVAKHESDFGFIEKPLETSGVQRYSLM
                     EDQLVIAGDPKSKLWLVREANSGVFHYTQRYFEEMNIQDKKMIVKSNDVIVALLKEGI
                     GRSILSKRSVPENVPMTPLNFHRYFYFIQRTNLVSDQLLEIAEEILRYNESTETSHSE
                     ENLSW"
     gene            23860..24825
                     /locus_tag="SCJ86_001071"
     CDS             23860..24825
                     /locus_tag="SCJ86_001071"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291188.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TDT family transporter"
                     /protein_id="HEG1168649.1"
                     /translation="MKNFLKIIPIPICGLILGLASLGNLFKDYHHVALGNLTGGISMI
                     LMIFIFVKLFILFEHTKQTLHDPIIASVSPTFTMSLMVICTYFVSYESIAPIVKFIWL
                     CAVIFQVILVFYFNYHHVIKADLSIEAIYPSWFIIYVGFGVITVTAGNFFPLIGKIFF
                     WISLICYAFLLPIIIKRVFFVRNMAHPTRPLITIVAAPGSLCLTGYLKNFAEPNFYLV
                     VALFALSQVLYFVVLSMLPRLFKLRFYPSYAAFTFPLVISATAIFTTVHYFNSLGIYS
                     SVLDFLKVAECLFASVVVFYVLAHYVRFLIKEHSKHGKFVSKKIA"
     gene            25202..26245
                     /locus_tag="SCJ86_001072"
     CDS             25202..26245
                     /locus_tag="SCJ86_001072"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375726.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative sulfate exporter family transporter"
                     /protein_id="HEG1168650.1"
                     /translation="MKSLSHPIKSVKDSAVREILPGLLLSFAIAAFSRLLAIYLPQLG
                     GATIAILLGILLGNTFFKQPVLAIGTKFAESKLLEFSVFLLGATVTAQTIAQIGVKGI
                     VFVLLQMSVTIIGAYLIGKKLQFSDTSSLMMAGGNAVCGSSAIASIAPSIHAKEEEKG
                     QIITLVNLLGTVMMLTLPFLGLQLFGGNLIEKSALLGGTLQSVGQVVAGASLLDNSVV
                     EFAMLFKILRIMMLVVVVYLFEKFMHKKQPALFETPTDTQKKRKLPLPWYVLGFVIFC
                     LVNSLFSLPVVIDQSAHFISSWFEITALAAIGLRLDFAKFMKEGTRFLSYGLAVGLLQ
                     TVMALLFIWLIKV"
     gene            complement(26307..26771)
                     /locus_tag="SCJ86_001073"
     CDS             complement(26307..26771)
                     /locus_tag="SCJ86_001073"
                     /EC_number="2.1.1.63"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337876.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methylated-DNA--[protein]-cysteine
                     S-methyltransferase"
                     /protein_id="HEG1168651.1"
                     /translation="MKIHTPIGFLWLDADESGLTAVSFQPISESESANQRILTRTQEQ
                     FEEYFAGKRKEFDVPLSIKRGTPFQQNVWKALSTIPYGEIRTYKEVAVAVDSPKAVRA
                     IGQANRTNPLPIVIPCHRVIGQNGKLTGYMGNAIDGVEIKRQLLLLEGYLQN"
     gene            27017..27964
                     /locus_tag="SCJ86_001074"
     CDS             27017..27964
                     /locus_tag="SCJ86_001074"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289773.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HEG1168652.1"
                     /translation="MKVSIVVPCFNEEKTVPLFFDEVEKFKGAHEFEYLFIDDGSKDG
                     TLAAIKKLASAYPYVRFVSFSRNFGKEAALYAGLQASTGDLVTVMDVDLQDPPELLPE
                     MINRITTSDIDCVGTRRSTREGEPAIRSFFAKKFYQLINKISDTEMVDGARDFRLMTR
                     QMVDAVLELTEYNRFSKGLFSWVGFKTEYISYENRERVAGETSWSFWKLFNYSIDGIV
                     NFSDAPLTIASFIGALSCVGSGIAILFIILRALLFGDPTSGWPSMVSIFLFIGGIQLL
                     CLGIIGKYIGKIFLETKQRPVYIIRETEKVKPNELKPHQ"
     gene            complement(28175..30247)
                     /locus_tag="SCJ86_001075"
     CDS             complement(28175..30247)
                     /locus_tag="SCJ86_001075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317775.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase III"
                     /protein_id="HEG1168653.1"
                     /translation="MKQLILAEKPSVAKDLSKVLGANQKHKNYYEGPKVIVTWALGHL
                     LGLKMPEDLNKEWQSWQMDTLPMIPKKLGIKPLPKTGHQLKAIKQLAQRKDVSEAVIA
                     TDAGREGELVARWILEYVHFNKPVKRLWISSQTDKAIKTGFKQLRPAKEYDALYESAL
                     ARAKADWLVGLNVTRALTVKYADNLSAGRVQTPTLALVRDQEKKIEQFRPQTYFTITL
                     QAGKEQAKMLQKNQFALKTQEEAQKLVQLISTSKGTVTEVQEKLKTESAPLPYDLTEI
                     QREANQRYGYSAKKTLSLVQSLYETHKIVTYPRTDSKYLTNDMKSTMKERLQAVSDFA
                     PEVKQYLKNGAIVQQTKVFQDSKVTDHHALLPTENRARYEKLSNEERKIYQMIVSRFL
                     GLFAQPHKVSQTKVTVEFGKERFIFRQNRVVQAGWKGETETETETVKWEKGMRINPDF
                     AIKKELSAPPKPLSEASLLGQMEKYSLGTPATRAEIIEKLIKSELMERTPQGLQVSPK
                     GKQLLVLVNPSLVTPDLTESWEKDLEAIAAGKKQAGTFLNGIEKETKRLVNEIKSSKQ
                     EYQDFSITQKKCPECGANLREKNTRDGKIYVCTNSDCNYRRRKDPKVSNHRCPQCHRK
                     MEIIEGKNGAYFRCKYDGTTEKLLDKKEQKKKMTKHEERRLLKKYAQAEEPEESPLAA
                     ALKAAMKK"
     gene            complement(30180..30557)
                     /locus_tag="SCJ86_001076"
     CDS             complement(30180..30557)
                     /locus_tag="SCJ86_001076"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1033 family protein"
                     /protein_id="HEG1168654.1"
                     /translation="MYQVITMFGDNEPWWFFEDWEEDIEEEETFESFEEARQAYEKQW
                     NEIHQNYEYINAKSNFLSAFWNDGDERWCEECDDDLQQYKGLALLKNHQPITVESRKE
                     FYETTNSSGKTKRCERPKQGAWC"
     gene            complement(30686..31264)
                     /locus_tag="SCJ86_001077"
     CDS             complement(30686..31264)
                     /locus_tag="SCJ86_001077"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286706.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HEG1168655.1"
                     /translation="MRGNMEMKKRNLSQEKIIDCFRELAEEMDVQQITFQHLAKRLDI
                     KAPSLYNHFKNIRDVKTALTAKLLQELNDQLRRALVGKSGQEALQIYAETYRSFAFSN
                     QAVYELLISVPHTNEAVLLDGIHETNQIILQIFEAFQLTKEEKVHRSRELRSMIHGYL
                     SLRFLGYFTKEPTIDPEDSYFWMINNFIATLP"
     gene            31396..32688
                     /locus_tag="SCJ86_001078"
     CDS             31396..32688
                     /locus_tag="SCJ86_001078"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286708.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MFS transporter"
                     /protein_id="HEG1168656.1"
                     /translation="MAARIKEKLFRKRETWEQNLFVLWFGTFMAGIGFSLVMPFMSLY
                     IDTLGNYSTSQLNFWSGLTFSSTFLVTTLISPWWGKLADQKGRKLMLLRASLGMAIVI
                     SLMGCVTSVYQLVALRLLQGIFSGYISNATALVATGTPKEKSGQVLGTLATGSVTGTL
                     LGPLLGGISASAFGYRPTFFITGIILFFVFLLSLFFVHEKFVPVKKENMVSAKQIFKD
                     LKYPHVVIGMFVTTMIIQASNNSISPIISLYIRQLLHGHGNVTLISGIIASIPGIATL
                     IAAPRFGRLGDKIGSERILAIGLGFAILVYIPMAFVTNVWQLAALRFLIGISDACLLP
                     AVQALITKYSPHQAAGRIFSYNQSFQATGNVVGPMIGSSVSSAFGYRGVFLSTSLLVL
                     VNFLLVRHNTQEIHQQETAEEKASGVSIASGHSPSHRQ"
     gene            complement(32748..33866)
                     /locus_tag="SCJ86_001079"
     CDS             complement(32748..33866)
                     /locus_tag="SCJ86_001079"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase"
                     /protein_id="HEG1168657.1"
                     /translation="MKIANFGVEEWLNKWEKKAVYDIAQSSIEALTLDELVGLDGTSV
                     SKFFEKRKNEPLDYGWIEGSDVFKQEVASLYQTVDPENILQTNGATGANLLALYALVE
                     AGDHVVSMLPTYQQLYDIPKSLGAAVDFVHLKEEEDWQFDLEQLEALVKPETKIICLN
                     SANNPTGTLLDRKTLEKIAEIARTVDAYVLIDEVYAPLTDKGEFLSIVDVYEKGIATN
                     SLSKTYSIPGIRIGWTATGPELAEVFRKYRDYTMICGGVLSDDLAVHALKNKEKILAR
                     NKKIITENLVILKQWVANEPKVELVAPNYVSTSFIKLAIEEDDQTFCINLLEETGVLL
                     VPGSAFDLPKHARLGYCCKKETLEKGLFLLSEFLKKQA"
     gene            complement(33880..35301)
                     /gene="arcD"
                     /locus_tag="SCJ86_001080"
     CDS             complement(33880..35301)
                     /gene="arcD"
                     /locus_tag="SCJ86_001080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286721.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginine-ornithine antiporter"
                     /protein_id="HEG1168658.1"
                     /translation="MEKKSTGISKWGLVALVVSSSIGSGVFGITSDLASSAAPGPAIL
                     SWIIVGIGILALVLSLNHLGEKRPDLDGGIFGYAEASFGKLGGFISGWGYWLSAWLGN
                     VAFATMLMSAMGEFFPIFKGGQNVPSILFASIFIWGLTLLVNNGVEGASVINTIVTIC
                     KLVPLFLFLIFVFIAFKVNIFTADFWGNVSDNLVNGTGQQGTIWLQIKGCLMVMMWVF
                     VGIEGASVLANRAKKRSEAQQASIIGLLCLLFIYILASILPYGVLSQEELATISQPAM
                     ANILKGIVGNWGAALINIGLIISIIGSWLSWTMLPAETTMLMARDGLLPKGWGKTNKK
                     NAPTYSLVVTAILTNLFLLTFLVTDYAYQFAYSLCTAAILICYLLVGIYQVQFSLKEG
                     IKKQVVIGGIAVVFELMGIILAGFSYVLLCSIAYLPGFCFYWIACRETKHQITGKEKV
                     MIGLICVAALLSIGLLAVGWIKI"
     gene            complement(35547..36668)
                     /gene="mnmA"
                     /locus_tag="SCJ86_001081"
     CDS             complement(35547..36668)
                     /gene="mnmA"
                     /locus_tag="SCJ86_001081"
                     /EC_number="2.8.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005880185.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA 2-thiouridine(34) synthase MnmA"
                     /protein_id="HEG1168659.1"
                     /translation="MTDNSKTRVVVGMSGGVDSSVTALLLKEQGYDVIGIFMKNWDDT
                     DENGVCTATEDYKDVAKVAAQIGIPYYSVNFEKEYWDRVFEYFLAEYRAGRTPNPDVM
                     CNKEIKFKAFLDYAMDLGADYVATGHYAQVTRDENGVVHMLRGVDNNKDQTYFLSQLS
                     QEQLSKTMFPLGGMEKSEVRAIAERAGLATAKKKDSTGICFIGEKNFKQFLSNYLPAK
                     KGNMVTLDGEVKGQHAGLMYYTIGQRQGLGIGGGGDSQEPWFVVGKDLATNTLYVGQG
                     FHHPALYATSLDASEIHFTTNEPMPKEFKCTAKFRYRQQDVPVTVRLLDDNRAEVVFD
                     EPVRAITPGQAVVFYDGMECLGGGLIDHAYQETKVLQYV"
     gene            37072..37671
                     /locus_tag="SCJ86_001082"
     CDS             37072..37671
                     /locus_tag="SCJ86_001082"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase family protein"
                     /protein_id="HEG1168660.1"
                     /translation="MNYFISDMHFFHERLLGKNDFAPRLFANVDEMNQQLITSWNAVI
                     KETDHVYHLGDLAMHPQYEKANAEILELVTQLQGTIHLIKGNHDSRAFFRYLEKNDPR
                     LTDGSPKFYFYDVGAIVKFDHQQYYLTHYPMLLGPNDTIRNLHGHIHHYSVPIENDVN
                     VGVDAPERELLKEKLPFGTPLSEQQIKEIYEAKRKYDRS"
     gene            complement(37809..39095)
                     /gene="efmA"
                     /locus_tag="SCJ86_001083"
     CDS             complement(37809..39095)
                     /gene="efmA"
                     /locus_tag="SCJ86_001083"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291207.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MFS transporter EfmA"
                     /protein_id="HEG1168661.1"
                     /translation="MENEQSVVLTNWKRNYLFFLSGQFLSGITSMVVQYAIIWYLTRE
                     TGSATILSFATLLGMIPMVLLSPFVGPLVDRWDKKALLIVTDIIVAIFALILAVVGTI
                     SESFPIWLVFVSLFMRSVAQTFQMPTIQSIMPTIVPSSHITRTNGQLGMVQSANFIIA
                     PALGAALFSVVPVNYLILLDVLGAVFGVGLLIFVKIPKVSPEILEVPLTIFKDAKFGL
                     QQLMDNKGLWYITINGAFVMLLFMPAISLYPLMTLDYFGGSVGQAGAVEVVYAVGMLL
                     GGALISFIGTWKDRMKPIIIAYIIMGLTIGASGLVPNDSQGFLYFLILNAGAGCATPY
                     FNTLLMAMIQQSYESNVLGRVLGNFNSLMNLTGPIGLLFAGPLADRLGVEKMFLFSGI
                     GILLCGIVLFLTSAARNYDKELQKKLVKEHHEQKDE"
     gene            complement(39366..39710)
                     /locus_tag="SCJ86_001084"
     CDS             complement(39366..39710)
                     /locus_tag="SCJ86_001084"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1831 domain-containing protein"
                     /protein_id="HEG1168662.1"
                     /translation="MAFETTATVLGAPISYRLHPDAKRYTLRDNGFTETNGGNYQLIR
                     PLDATPQSKEGFKLKITVSKDIQTLKMSITTANGLKAVNIFKDPKQKMSQDKFYFLMD
                     GMISRGLFEKVE"
     gene            complement(39847..40992)
                     /locus_tag="SCJ86_001085"
     CDS             complement(39847..40992)
                     /locus_tag="SCJ86_001085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631303.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine desulfurase"
                     /protein_id="HEG1168663.1"
                     /translation="MNEVKTIYLDHAATTPMHPQVIEAMTAIMQDTFGNPSSIHGFGR
                     HAHEKLETARQIIADSLQAKPHEIIFNSGGTEGDNTAILETAFSRQKEGRHLITTAIE
                     HPAVLNTMKYLETKGFDVTYLPVDEKGNLSIEEVKKALREDTILVSVMYGNNEIGNLM
                     PIKEIGELLADHPAYFHTDAVQAYGNQTIHPEELGIDLLSISAHKINGPKGVGFLYKR
                     DGVNIPALLLGGEQEEKRRAGTENLAGIWGMAKAVELLSPEEREARNNKYKGFQQLIL
                     DTLEANEIPYQVNGDLTNKLPHVLNLRLIGEANDLLLMKLDLNGVAISTGSACTAGNI
                     EPSHVLEAMYGEDTPILKESIRISFGLGNTEDDIQYFLDQLVRAVKK"
BASE COUNT        11846 a         8649 c         7193 g        13467 t
ORIGIN      
        1 aaaagcaaaa aaaaaaagaa agaaacttat tcttgctaat aaactacgcg aaaggagtct
       61 aacgttgttt gatataactt agactgatcg ttaacttatt ttttatattt ctacatagtt
      121 atctttagaa ccatttcaga tacatatgaa acaattacat atgatttttt cataaaaaaa
      181 cagtactgtc tagtagtata aaatatacaa atttcttaaa aattttttca ctaaaatcac
      241 tacctgaaac agtctaaaac acttatatat caacgtttaa tgaaactatc tccgtttcat
      301 ttcagataca aataaatata gtcgggctcc aaatccgaaa aaaaataccc ccagtagctt
      361 atcctaagct actggagatg tataagtacg gtcatataac aacggggtag ggtaaataaa
      421 tgaaaaagat ggaagaatca ttattcaata acttttttaa atacttcgtt gtaatcagtt
      481 acctgtccgt tcttaaatac cacagaataa tcttttttcc cattttcaag taaccgaagt
      541 ggaatgtctt taccaacttg ctctgtcact gcattactga tacctgttag attagctaat
      601 aaatctgata aagattgagc atctgtagaa atctctgtat cagtatttat cgtgattcct
      661 ttatcttcat ttacaagctt ccaagtaggt ggtatagctg tgtatgtttc gatgaatgaa
      721 tcaattcttt catctatcgt cttcccagta tctacttctg tctcattact cttagatgaa
      781 tcgattcttt catccatcgt ctgctccgta actgcttctg tctcattatt ctcaccggaa
      841 actctcttca cacccaaact tatatattgt ccatctttag tgtgcatagt taaaattccc
      901 acgcttttac taataaattc actataacca gttctttcaa tagagacttc aatgtatttc
      961 acactatcat actcaaaaga aaaatagaca tgtaaaagca ttttgttgtt tacatctaca
     1021 taataatcct ttgactctac ttgagacctt cctatacgga atacagcttt actgtctgat
     1081 atctctatac cagcatcctt attattaatc tgccaatctc caagtagctc ttctggcaag
     1141 ctagtagttt cttgtgaaca ctttaccact tcactggtag aggtcgaaac attcacctcc
     1201 gcactgttag aagaattttc caaggaatca ggagccttag cacaaccaac aagtctttat
     1261 atattgtgca gccgatattt tcttcattca gtacaccctt gaaatatgca gtgttagata
     1321 aagcagttcg tcctgatttg ctgaggcacc catctcttct tgaacgtact ccttgatttt
     1381 caaggccgcc cgatactctt tcggaaaacg ctgcttcact gaagccagca aaaaatcatc
     1441 cttctccggc aactcttcct tcttaatcat tcgggcaata aaaaactgta aatgcgtcac
     1501 aaatcttaag taaagcatcg aatctttatc aacctttttt tcgggaaaca gatatcctag
     1561 catagccaag atatccttta gtgctcttac cgagagcatg gtttcttcca tatttgcttg
     1621 ttcactttgg gcatttacaa tgtgaaacgc aatgtttcca gcctcctctt ctggcaactg
     1681 aacattcatt tttttgttga tcaattggat agcatagcag ccgatctcaa attcttttgg
     1741 gtaatacttt tgaatttcaa tcagcaggcg attttgaaaa acaatgcctt tttgactgcg
     1801 ttcaatggca tgcttcaagt gatcaaccaa ggtaaaaaat agacttttat tcagcttaaa
     1861 gtcaaacttg gcgttggcat aatcgataat tcgcttgacg caaataatta gatcatccgg
     1921 agcagcctca aacagtttaa tgtaatcctt ttttagagca tcagaaagtg aatctaattt
     1981 aaatactttt tggattttgt cagccggaac caaatcgcct ttctttagtt gaaatccaat
     2041 ccctttcccc atcaaaatta cttctttgcc ttgcttatct aagcaaagaa ttaaactgtt
     2101 gtttaacgtt ttaattactc tcattagatt caccgttctc tctaagctaa gccatttctt
     2161 gcgattactt tttgatacca aaagaagctt tcttttttta ttcgttttaa ttctttaaga
     2221 ttctcatcgg ttcggtcaac atatataaaa ccataacgct tattgaagcc ttgatgggta
     2281 ctaacaacat caatcgctga ccaaggtaaa taaccaaaaa catctacacc atctgttaaa
     2341 gctgcttgaa tagccagaat atgtttggct agataatcaa ttcgatcctg gtcattaatt
     2401 cgtctatctt cctcaactat gtcgggtgcc ccatagccat tttcagtaat taaaataggt
     2461 aaatcatagc gatcgtacaa tttcctcaat gttaatcgta gtccagtagg gtcaactacc
     2521 cagccatatg gcgttttatc tacataagga ttttcagctg cccggtagac accttcttct
     2581 cccaacatga tctgttgatc ccccgctcta gcggaaacat ctgatccgtc atttttgctg
     2641 gcagcgatgg tggcagttga ataataatta atcgcaataa aatcgggttt accggaggat
     2701 aaaattgcct gatcctctgc ctcaatttta ggatataatc cacgatcatt caaatagttt
     2761 tcaaacaaat aattgtattt tccccgaact gctacatcta agaaagacca gcctctcaat
     2821 gtttcccaat tatgggctgc aattgcatct tctggactgg aggtagcttg atacatagcc
     2881 gttaaattga ttgctggacc gatttttcct ttaagattca actgatgata caagtgaatt
     2941 actctagctt gagctaataa aacatggtga ttaatttgaa agagttcttt ttttgaaggc
     3001 agctctccac ttggtggcaa accaatcgct ccaggatgaa ggatcatcgt gttttgctca
     3061 ttaattgtca gccaatagtt caccttgtca ccatactcag taagcaatac ctgtacatag
     3121 tccacaaaat aatcgatagt tgctcgattt aaccaaccgc cctgctcctc tagaaaataa
     3181 ggtaaatcga aatggtacat ggtgacaatc ggttcgattc cctgattcag gcattcatca
     3241 attacttgat gataaaaaga aattcccttt tgattggttt tattattttc aatatccata
     3301 attcgcgtcc aagcgatgga aaagcgaaaa gcttttaagc ctaattcttt aaacagctgt
     3361 atatcttctt tatagtgatg gtaaaaatcc gaggcaactt caaaattagc taccccttct
     3421 gtatgctgat acttgtcaat aatagacggt tgtttcccat cctcctgata ggcaccctct
     3481 gcctggtagg cactaatgga ggcaccccat aaaaacttct ctggaaatgc tgaaagctgt
     3541 ttgtattgca caaaaatcct ctccttaact aatttgcatc aatggttcgt gttcctttaa
     3601 gcttccgctt gcttcactaa ttcctgcgta attttcactg ttggtcacta ccacaatcac
     3661 tgtatcatcg tagccagcgt ttctaatggc agcgaaatcg gcgttgatta ataattgtcc
     3721 tgctgacacc tgatctcctg cttttacaaa tacttcaaag ggttcccctt ttaaattcac
     3781 ggtgtcgatc cccacatgga tcaataattc aataccttct ttagaacgta gaccaatcgc
     3841 atgtttagat tctaaaatag taatgacttc tccctcaaca ggtgaataaa tgctgttttc
     3901 ttctggatga atggctacgc cctttcccaa tagtccctta gaaaagacag catcgttcac
     3961 ttcagaaata ggacacacgg ttccttttac cggattttca acaatgactg cttcggctat
     4021 tgccgtttgc tctgattcat cagaagtatc gtcaaatcca attagataag caatgataaa
     4081 ggttgcaaca aaggcgatta cacacgtaat aattgcgtga acaatattca ttggattttc
     4141 tccaataaag gctggtaagg ctgctaagcc aggggaaaca aaggcatatc tgactaaacc
     4201 gctcaagcca gcatacaaac cagcaattgc accaccaatc atggaagcaa ctaatggttt
     4261 tttcagcttc aaggttactc catagagtga aggctcagta attcccatta aagcagtgat
     4321 cccagtggac acagctagct gcttcaactc tttgttttta gttttcgcgg ctacagcaaa
     4381 ggtagcacct gattgagcaa tattagaagc cagcattcct ggcccattga tcatttcaga
     4441 acccgtactg ctcaattgca tagtagcgat tggcgtcatt gcccaagctg tacccgtaac
     4501 caccaaaaat ggctgcaaac cacccatcaa aaatggaatt aaccaagctg ctttgccgtt
     4561 aatccattca gcccccattg ctataagatc gttcagcaac gttccaaacg gtccaatgac
     4621 aaccaacgct aaaggcccca taattaaaaa taccagcatc ggcttagtaa agaatttaat
     4681 aaacgaaggc gaaattcgat ccgcaaaggg ctctacatag gacatgatcc aaacagtaaa
     4741 gataatcggt aaaactgatc ccgcataatc tgcagctaat accggaattc ctagaaaaga
     4801 aacggcatcg ccagtagaaa aaagctgtga aatatttgga tgaagcaaaa tgccagcaac
     4861 agtaatcgcc aaaattgggt tgacatcaaa ctttatggct gccccatagg ctaatagtac
     4921 tggcataaaa taaaaggctg catcagcaat agtattcaaa atgatgtaag tttgactact
     4981 gtctgatagt acaccagtca aagtcaagac ggccaaaatg gcttttacca tcccggcacc
     5041 tgttatagct ggaatcatcg gtgtaaaggt ggtagaaatg acactaatca atcttgaaat
     5101 aatactttct tgattgtctg aagaggggcc ctttttaatt gaagttccca cttccttttg
     5161 aatttccttg aaggctgttt ggacttcatt gccaatcacc acttggtatt gtccaccgct
     5221 atccaccaca ctaattacac caagtgtttt ttctaaggct ttcgtatcgg ctttcctttt
     5281 atctttgagt tcaaaacgca gtctggtggc acaatgggtt aagctgacca cattttcttt
     5341 tcccccaacc tgttgcaata catttcttcc tacttctctg taatccatta ttctctctcc
     5401 ttaaagcagt aaaaaagacc aagtcaaacc acgccccaat caacattgaa gtgttagttt
     5461 gtcttggtct tgcctgcata atcagtaaca tgccaataac gttattttgc tgtaatccaa
     5521 taatagcaca ttatgatagc gcaatcaaca gggaatttaa ttttttatct tttcgtttga
     5581 ccgtataggg taatataaca gttagatcac atcaactagg gtatatctgc gtatacttac
     5641 tgctaaaata agtatatcta atattaacta taccagtaaa accattccta cataaccagc
     5701 gggaaattcc caaaatcgtg aaagcatcaa aaatgcatat aacggatcac acaacttcaa
     5761 caattctgct cttgggaaaa attgatgaat ttggtgagta aaggatggaa taaacagcat
     5821 tccaacaata taggcaacaa tagtgatact tgtttttttg gtaaaaatca gtaagcaata
     5881 gccaatttca atcattccga gatataaagg tatcgataga atgatttggg ctagtattct
     5941 taaaaattaa ctagcaaatg tttaaactga tttatttcta atctacatgt agtaagtatt
     6001 tcaattccac caattatcaa taaaaatacc aaacatatcc ataataattt tttctttttc
     6061 atttctttta tattgttata acagtgaata acataaaaat tcccgacaat cattgtaata
     6121 tttttttgtc acctgtatta gtatttaatt tataggaaac tataaaaact ccctagtgaa
     6181 tttacacctt tgggagttcc tgtttggacg gtatgcataa tacatttaaa tcaatttaac
     6241 attaacacct cagcatatgg tatctccaca tatatacggt ctttctttgg atagtactca
     6301 atatttatgt tgatagcact tccattgtct aatatagctt tagcccggta tgtgttatct
     6361 ccattttcat gagtaatcgt cacatcatca catgatcgat ttaagttgta ctgttcttgt
     6421 aaaatctgtg ttaccaatgg ttttgatgac ttctctaatc cagatgtacc tgtcattttt
     6481 gaaaaaataa tacctataat agccaagaca acaataccta taatgacaaa cttcttgttc
     6541 atatttactt tattcaactt tttatctcct atctccaaaa taatttgctc ttatccctct
     6601 tatcagtaca ttgacatccc taaaagtaca ccaaagattg ctccagctac ccctgctatc
     6661 atcaaaatcg tacccatttt ctcatctttt tctcgatata agtagcccat aataacagca
     6721 acagcaccaa atacaacagg aataaatgct aacgatacta ccatagaaat ccaaccaata
     6781 atcaaataca gcttccctgt ctcttgtcca tgtacgattt gttccttcat ttcattaatt
     6841 gtctgctcag tacttttctt tactgtgtct gttccggttg tctcatctgt ttcttgtagt
     6901 gctccacaaa actggcaata cttactttca tctgttattt ctttgccaca ttttctacaa
     6961 tacatgtctt ctcctcctaa aatcattcaa ttgcttatta gttaactgtt tactttatta
     7021 aatacatcag aaaacagctc attcattggt acacttaata tctcagaaag catcttcatt
     7081 tgatagggtt taggaatcgt tctccctgtt tgccacgaac ttacacattg ctgtgtaaca
     7141 ttcatttttt ttgctaactc cgtttgagaa agtccttttt cttttctgta tcggctgaga
     7201 ttacttactt ttttcatgat tctcccctcc tgttttatta tatacaacaa tatgatgtat
     7261 ttcaactcat aaaatataaa aaacttcaaa taccattaca aatacaacaa cttgttgtac
     7321 tatactgaga tgggggtgta ttaatgaaac agaaacaata tgcaaaggtc attagagaaa
     7381 agcgaaaagc tctcgggtgg acacaaaaag aattagctga aaaaatcttc tctacacaac
     7441 aggcagtagc aagatgggaa aattctgtaa cagagcctaa cctcgatagc ttaacagctc
     7501 tttctagagc cctaggaacc cctgtaagcc attttctaga caatgttgtc gtagattatg
     7561 aggaagagtt tttggctctc tatcgttctc ttagcactga agatgcagtt cgaacaattg
     7621 actatatgaa gctattaaaa aggcaagaaa atgaacgcaa tcagctattg aaagattgaa
     7681 aaataaataa cccaccgaac tttaacggtg ggtgtattga gatgaaataa gattaagagc
     7741 tatggataac ctgtctagtt taatacaatt gggtcttttc tcttccttac tctccgtcca
     7801 tcatctcttg ttacacttgt aaattgatac agaggaacta tcccagctcc tgataatatc
     7861 aattctttta ttgtttcatc tatccctgcc cctttcgcta aatatattaa aaaactacta
     7921 cttgctttca cattgtatag tttcttaata tccttagaag ctcgttcaag agtgaatcct
     7981 tgtttaacaa agtagcctct gttttttatt aaagcatttt tcacgctgtt atccccaaca
     8041 ttacgtaggc gttcacaaac agtgttaata agttctttct ctgtcattag atatgtccct
     8101 tgccacgata agccctgtaa aacttcatgt gcttcatcta aaatcaattg tttttccttg
     8161 ctgagttctg atcgggaatt ttgaggaaat actcttttag cctctttttc gttaaaacat
     8221 atcttaaccc attcataaga aaaatattga tatgtaaaat gaattttttc cagaagtgga
     8281 aggatatctt cattcaattt ttgaatcaca gaagttgcat taaaaacgtc taatcgatag
     8341 acattgcttc tcttatgatt aaagtttcct aacgctttct cttcctttaa cctatttagg
     8401 tattcgagca tcttcaaagg aacatcttca tcatctaatt tttttatcaa tcctataaaa
     8461 gtcataagtg atagtaactt attacattta tctatgtcat aagttttacc atagtaagaa
     8521 ctcatttttt tagctaattg ttcccccgac aaaatacaag tcacactttc cttactatca
     8581 tcaaaaaaat taacatcaga aagcaagaca tcatagacac gtcgcatttc ctctagataa
     8641 ttccttattg tcctgaaaag tttgtctttc attgattcaa gagtgtcaaa aagtactttt
     8701 gattcctctt tcaattttcg aaaatgtgta ggatacaagt ctaggttctc taatagccaa
     8761 ttaaatgttt ctacccgact gtctttcttt aaaattagca tcacaactcc aataaaattt
     8821 tcactgatat cacaattact ggaatgacaa taaaaaatgg aatgcccagt tttactggta
     8881 aaaatactag cagagggatt atgatcgttg tgaaacagac atcttaagtt tttacttgac
     8941 ttaagtaatt tattcatatc aatggttaac aatctttcat aaatctcatt aaaagacatg
     9001 tcggaggaat caaacaggca gtctttaaac cactgtttca tttcttcttt gtcgtcattt
     9061 tttatcaact taacaaaatc ggtttcattg actagttcat actctctccc tgaatcactt
     9121 tgacaatttt tagaacatgt catggtaaaa tcaatcggaa gctcagtaat atctaacatg
     9181 ttgtcttcat taattattat cactgcatct gacttatatc ctccaaaaaa cattctattc
     9241 gaagcattta cattgtcatc acacccgggg atgttctttt tcaagtattc gtaaacagca
     9301 accattttac catagtcagt aattgcttct tttaatagaa agacaatctt gtatttttct
     9361 agattttcag agtaccgaat acttttttgt atcgcaaacg cagaattatg aactaaattg
     9421 atagataaaa ccttttctac gcttaagtat ctactatcat ctttactcaa atctactacc
     9481 ttccccttct catctttaac agtgttatcg acatccagaa taattactct ggtttccaca
     9541 aaactctcct tgtcattgcc tttattcttt gtcttgcttg gaataaatga ttgtgatgaa
     9601 tttagtatct ctacaaactc atctcttgta acttctctat agttatctaa acacctatct
     9661 cttacatcag ttttttcttt ttttgtaact ggcttctcta ataatttttc ttgatctaca
     9721 taaattggaa tattaatcat tgatgtttcc atttgtgttt cctccttata aaattctgtt
     9781 aaagactaaa acatacagat aataaaaaca aactctttat tactatatgt tttagtctta
     9841 tttgtttact caacctttat accgttccat gaaactatca atatcagatt ttttaaatct
     9901 aatgaccgta tcaattttta taacaggtaa cccgtattga gaaacatatt ttctaatcgt
     9961 tcctactgat acacctagat attcacttgc ctgctgatag tccatatact ctagagtgac
    10021 tccagaaata atgtcttcta gcataggctt tagcactgta attatctctt tcacttgttg
    10081 ttcaacttgt atagaaacaa tgctatcaat gatttgtaga gacatatttc cccctccttt
    10141 cgtgattgct tgaacaaatt ttctaagaat tatcttcgta ttttttgctc aaaataaaag
    10201 tattttgtta ttaaccgatt aatcaaacaa aaaatgatta ccagttcatc gacatacaca
    10261 taatacctcg ataaaaaagg ggtgtcaaca gctttgtaaa atttatttgc aacacccctt
    10321 ttttgaggta tactaatttt aggaggtgtg gtcaatatga taaaacgtcc accaataaat
    10381 tatttagaac gaaaaaagat actcggaaca aaaataaaag ctataagaaa gagtaaaaaa
    10441 ttaacacaac cagcatttgg tcttatgatt aacaacggtc aattaattga caaaaaaact
    10501 atctacgaat gggagaaggg aacctattta ccaataccag agcgattatc ccgaattgct
    10561 gatttgggaa atatgagtat agaggagtta gtttgtggca atgtagaaga atatattttg
    10621 ggaattattc tttatagaga tagcattgta ctagatggta taacatttcc agataaaaac
    10681 ctgttccagc atcttcgtca acagttccca ccagtacact ctaatttaga tacatggctg
    10741 gatagatatt ccaaattaga accagagatg caagaattta ttgctaacaa gacttgtaat
    10801 aaagttaaaa atgaaaaaat tagtcttttc aatatcttaa aaatagaaga attattcatc
    10861 aatgctattg tagaagaatt tgacaataac attcttttcc tcactagcag tatagaagaa
    10921 ctactagaaa gaatggtaga tgaatggcta cctattcaat tgaaagacat gtcttatccc
    10981 gaagaagctg tgagagaaat tactgataat ataaacaagc tagaacaaac gatttcatca
    11041 attgggaaaa aatacactaa gaaaaaaatg aagggaggtg ataccatcta gtggtaagta
    11101 ctccttgtaa actaagagga gatgatttaa tggcaaaaat atcaaagtac aaaaagaaaa
    11161 acggtcaagt ggcgtggatg tataaaggac acattgccac cgatccacga actggtgaaa
    11221 aaataaatac tacgagaaga ggttttagta gcaaacaaga agctcaaaaa gattatgacg
    11281 agtacagaca tcaaatatta tacggtgtta aaaagaaagc acctgatatg acatttgaag
    11341 acctgtataa tgaatggata actcatcagc gtaccagtgt taaagcttca acaatcgcaa
    11401 tctcagtaag atacgcaaat aatcaaatac ttccagcatt tggtaagctt aaactatcga
    11461 atatctctgt cccatattgt caaaaagtgg tagatgaatg gcacagtaaa tatgaaagtt
    11521 acgattatat gagaaaacag actgcacaaa ttttacgcta cggggttgca atgcaatata
    11581 ttgacaataa tcctatggaa aaaacacttc ttccacgaaa aaaagagtat gagaaaaatc
    11641 gtaaatttta ctcaaaagag gagcttaata atttactgga tgcttttaaa gattttggta
    11701 acatgaaaca atacgccttc ttcagattat tggcatacac aggtatgaga aagagtgaag
    11761 tactcgcttt acagtggaaa gacatagaca cgtttaataa agaactacat gtcaataaaa
    11821 ctcttgctgt agatgaattt ggtaaagtta taattcaatc tccgaaaaca agagcttcta
    11881 gacgagttat ctctttagat actgagactc tttctatcct caacaactgg aaattacagc
    11941 aaaaagaaga gtatttaaaa cttggataca atactagctc taaagagcaa catgttttta
    12001 caacagttaa aaacactcta tacattccaa acactgttaa tgattggtta agatatattt
    12061 tgaaaaaata taacttgcct cgtattacac ctcatgggtt tagacataca catgctagtt
    12121 tattattaga agcaggagaa tctgtcaagg tagttcaaca acgattaggg catgaaaata
    12181 gtaaagtgac attagacatc tatgcacata ttacaaataa tgcacctaag aaaacaggtc
    12241 aagactttgc tgatatgatg gctcatcagt aaaagagctg gtcaaaatct ggtcatttct
    12301 agtatacagg caaaacaaaa aagctagaac ccttatatat caagggttct agctcctata
    12361 acaggtttac ccaacactgc cttccatctc ataactgatc aatcgattga gttcgactgc
    12421 atattccatt ggaagttctt tggtgaatgg ttcaacaaag cccatgacga tcatttcagt
    12481 tgcttcagat tcggtcaggc ctcggctcat cagatagtag agttgttctt ctgagatttt
    12541 tgagactttg gcttcgtgtt ctaaagagac ttggctgttg tggatctcat tgaatgggat
    12601 cgtatcggat ttcgaccatt cgtccatgat gatcgtgtca cattcgatat gagagatcga
    12661 acccgcactg tttttggcaa aggttacttg tcctcgatag tttacttctc cgccgtcttt
    12721 tgaaattgat ttagagacga tagagctaga tgtatttggt gcattgtgga tcatttttgc
    12781 acccgtatct tggatttggt ttgctcctgc aaaggcgatg gagagcatgg tgcctcgtgc
    12841 accttttcca tctaagtaaa cacttgggta tttcatcgtt gtttttgcac ctaggtttcc
    12901 gtcgatccat tcaacggttg cgccttcgta tgcttttgct cgtttggtta ctaagttgta
    12961 cacattgtct gaccagtttt ggatcgttgt gtaacggcaa tacgcatctt ttcttgtaaa
    13021 gatttctacg atggcggcat gcaggctgtt acttgaataa gtaggtgccg tacaaccttc
    13081 gacatagtgg atgcttgctc cttcgtcaac aatgatcagc gtacgttcga attgccccgt
    13141 gttttccgca ttgatccgga agtacgtttg taatggaaca tcgactttga cgccttttgg
    13201 cacgtaaata aaggtcccac ctgaccatac ggctgaattc agcgctgcta atttgttgtc
    13261 ggttggcggt acaagttttg aaaagtactc tttgaataat tcagggtatt ctttcaaagc
    13321 agagtcagtg tcagtaaaga cgattcctaa cttttcgaat tcatctttca tattatggta
    13381 cacgacttct gattcatatt gtgcggaagc accggctaga taagctcgtt cagcttctgg
    13441 tataccaatc ttttcaaatg tatctttgat tttttctggt acgtcttccc agtcacgagc
    13501 gggtttgtca cttggttttt ggtaatattt gatggcatcg aaatcgatgt ctgaaaggtc
    13561 ggggccccat gtttgcattg gcattttgtt aaatgtttct agagatttca gacggaattc
    13621 aagcatccat tctggttcgc ctttgattct tgacatctca cggatcactt cttctgtcaa
    13681 gccttctcct gtactgaaaa ccggttcgac atcatcgtga aacccgaatt tatattcttc
    13741 taattctggt acgcccatgt tctcactcct cttccttgtg gtaatgatgg aaagctgcgg
    13801 taccatcgtt ttccattgct tttcctagtg ctttccaggc aagtgtcgca catttgatcc
    13861 gagcgggaaa tttagctaca ccaccaagca tagcagcatc gcctaattgg tcgggatctt
    13921 tgacttcttt tccttggaca agctgtagaa agtctacagt taaatgttct gcttcttcta
    13981 ttgattttcc gatgacggcg tctgtcatca tcgaggcact tgccgtactg atggagcagc
    14041 cactgccgtc aaaggcgata tttttgatga ttccgtcttt tacatcgact tgcaattcga
    14101 tcacatctcc acatgtgggg ttgttcaatt cgatcttgct tgtcgagtca gtcaagcttc
    14161 cacgatgatg cggatgggaa gaatgatcca agatcacttg acggtataaa ttgtctaatt
    14221 tagatagtcc catgttggaa aaactccttt gttgccatga ttgcttcaac taaacggtcg
    14281 gcgtcttgcg ttgtgttgta gaaataaaag ctcgcgcgag cagtcgctga tacttcaagg
    14341 tacttcaata gcggttgcgc acaatgatga cctgctctga cggctacccc ttccatgtct
    14401 aaagctgtcg cgacatcatg gggatgtaac ccatccaaat tgaatgcaac gactcctgtc
    14461 cgaaaattcg ggtcttgtgg accataaaca gtcaatccat caattgcttg taattttggc
    14521 aacacataag cggctagttc ttcttcatat cgatggatat tgtccatacc gagtttttgc
    14581 aaataatcga ttgcatggcc taatgcgata gcgccggcga tatttggtgt ccctgcttcg
    14641 aatttccaag gtagttcttt ccatgtactg tcgtataatt cgacaaaatc gatcatttcg
    14701 ccaccaaatt caacaggttc catttcttct aaccattttc gtttaccata taaaacacca
    14761 atacctgttg gtccgcacat tttgtgtccg ctgaaagcaa agaaatcaca gtctagtgct
    14821 tggacatcaa tggccatgtg cggggcagat tgagcaccat ctactacgat gaccccgccg
    14881 ttttcatgga caagttcagc taattcttga acaggattga agacacctaa gacattggat
    14941 acatgagcaa tggagacgat tttggttttg tccgtaatct ttttcgctgc atcctccata
    15001 tccaggaagc cttcttctgt tagttcgata tatttcaaga cagcacctgt tcgttttgcc
    15061 aactgctgcc acgggataac attagaatga tgttccatgt aagagagcac tatctcgtct
    15121 cctgctttga tatagcggtc gccgaaggtt ctggctaccc aattcaagct tgtcgtcgta
    15181 ccacgagtaa acagcacttc tgctgtttct tttgcatgga tgaacgcccg tactttttct
    15241 cttgcttgtt cataatcatg ggtcgcgcgt tctgctaacg tatggacacc gcgatgaaca
    15301 ttggcgttat cgtgttcgta atagtacgct aatgcctgga gtacttgttt tggcttttgg
    15361 gtcgtcgctg cattatccag ataaactaaa ggttcatcat tgacgtcttg aaataagata
    15421 ggaaaatcat tgcgtagctt tttagcatcg atcatccatt caacttccct tcgattactt
    15481 cgactaattc tttccgaact tgttctacag ggattgctgt taagacagaa ccgaggaatc
    15541 ctcgaatcac taagcgctct gcttcttctt tatgcaatcc tctactcatc agatagtaca
    15601 tttcttctgg atcaactcgt cctacactgg cagcatgtcc agcagttact tcgttttcat
    15661 cgatcaaaag aataggattc gcatcaccac gtgctttatc agaaagcatc aagacacgac
    15721 tttcttgttg ggcatctgca ccttttgccc ctttcaagat gtggccgata ccattaaagg
    15781 tcaatgttcc tttttctcgg atcactccgt gttgcaaaat atgcccgatc gtatgagggg
    15841 ctttattggt tactcgtgta tcgattcctt ggatttgtct gcccgagctg attgccacga
    15901 ttttgacttc tgcgtgtgca ccttctcctg ctaaatctga gtcaaagtca gcgatcacat
    15961 gaccgtcatt catgacacca attgcccagt cgacagaagc atctcgtaaa atgtgcccac
    16021 ggcgattcat ataagaagta atattttctc ctaattgatc tacggctgaa tatttgattt
    16081 ttgaaccagc tttagcgatg acttccacga tgatattacc tgatgatttt gctacttgtt
    16141 ctttggttgt ctggaaacgt tccagataag aaaattcact atgttcatct gccacgatca
    16201 acacatgctt gaagaagtgc tcatcggatg cgccatcttg aatgaataag gactcgagtg
    16261 gttcatcgat cacgacattt ttaggcacat acaggaaaat accgctgttc ataaaagcta
    16321 catggagtgc agtcaattgg tcttcgttca tgtttactgc ttttttcata taatattctt
    16381 ggactagttc tggatattcg atcattgcag tgaacaagtc ggtaaaaatg actccttttt
    16441 ctgctaattc cacaggcagt tgttcaaaaa tcgtccatga accttgttgg acaagcacag
    16501 gattgtcttt cattgcatca aatgccggaa tcgtgcctgt ttcactgatg gtttgtgttt
    16561 ctttcacatc aaataacgac cagcgatgga atttcacccg atcgatatgc ggcaatggca
    16621 gttcatctgc tttcgctagt gcgtcttgac ggaaggttct catccaatca ggttcgcctt
    16681 tacttaaaga aaaatcattt accgcgtcaa gataatcgag ccagtttttt tctttcattc
    16741 ttgctccccc ttacgcttct tcttctttat agtcgattcc taattcttta ctgataccag
    16801 cgtagccttc tgcttccaga cgtttagcta ggtcagcatt ccctgtcatg actactcggc
    16861 cttccatcat gatgtgaacg acatcaggtg tgatatagtt caataatcgt tgatagtggg
    16921 tgatgatcaa tgcaccgaaa ttgtctccgc gcatttcatt tactccttta gcaacgactt
    16981 tcaaagcatc gatatccaga cctgaatcga tttcgtctaa aattgcaaat gtaggttcta
    17041 acatcaacaa ttgcaggatc tcattccgtt tcttttctcc gccagaaaaa ccttcgttca
    17101 gataacgttc agccatttct tctggcatat ttagtaaagc cattttttca tctaactttt
    17161 tcaaaaatgc cataacagaa attttgtcgt tttcatcacg tttcgcattg atcgctgcac
    17221 gcatgaattc cgcattcgtg atacctggta tctcacttgg atattgcata gctaaaaata
    17281 agccaagtcg cgcacgttca tctacttcca agtctaaaac atttttcccg tcaaacaaaa
    17341 tttcgccttc tgttacttca tagtttggat tgcccatgat agcggcagat aaggtagatt
    17401 ttcctgtgcc gttaggtccc atgatggcat ggatttctcc ggttttcatt gttaggttta
    17461 cgcctttaag gatttctttt tcctcgatcg acacatgtag attttttatt tctaagacag
    17521 acatgcatta tacccccaaa ttctctttcg ttctcactca ttttagacta attgagaatg
    17581 aagcgcaatg accttcctta ctaaaatcaa atatctttta tttattatta aacaattttg
    17641 atttctaaat tagaataggt ataaaaaaga tgagaaataa gccattacag acttatttct
    17701 catctttttt gttccatacc ttattttgca tcagccttta ctggtttaac agagccgccc
    17761 catttgtctg tgatccattc ttggacttct ttgcggtgaa gcacttcaat cagttttttg
    17821 attttgtcac tgtcttcatc acctttacga gtcgcaataa tattcacgta aggagagttg
    17881 tccttttcaa gcaatacggc atcttctaat ggattcaacc ctgcgccgta agcgaagttt
    17941 gcgttgattg caactaggtc accttcatca ttttggtacg tagaagctaa aagtgatggg
    18001 tcgatcgtat ggttgaactt caagttctta gggttttctt caatatcatc gaatgttgct
    18061 gtttccagat ctactccatc tttgactttg actaagccag catcttgtag aattgtgatg
    18121 atgcgtcccc agtctgattc agaatttgaa gtaatgattg ttgcaccatc ttttaattcg
    18181 gaaacatctt tgactttttt agagtacaag cccattggtt cgatgtggac agcaccggca
    18241 ttgacaaagt catatccttt ttctttgatt tctttgttca aataaggaat atgctggaag
    18301 taattggcat caatgtcgcc ttcttccaaa gattggttcg gtaatacata gtcatcgaat
    18361 ttgacgattt caagatcgat tccttcatct ttcaataatg gtttcacatg ttccaaaatc
    18421 tctgcgtgcg gtgtcggtga agcgccgact ttcaatgtcg tgtcgtctgc tttatttgct
    18481 gaatctttgc tgtcgctgtt gttaccgcat gctgctaatc ctacagtcag caaaagcgct
    18541 gctgcaaatc caaaaatttt ctttttcata tcctacatcc ccctatatcc tattatcttt
    18601 tgtctgtttt cttagttaag aaatcaccga ttccttgaac gataaatacg attagtaaaa
    18661 taattaaagt cgcaacgaat gtcactgtta gattaccacg ctggtagcct tcctgccaag
    18721 caagacctcc aagtccacct gcgccgatcg cgccggccat cgcagtaaat ccgatcatcg
    18781 agatcgttgt aactgttaat ccagaaatca atgcgggaat actttcaggt atcaagacct
    18841 tccagatgat ttcccagtaa cttgctccca ttgcatctgc tgcttccagt acaccagggc
    18901 tgacttcacg aaaagcaatc tctactagac gggcataaaa tggtgctgcg gataaaacaa
    18961 gagctggaat ggctgcttgc gcacctagca tagtgcctac gattgcttta gtaaatggaa
    19021 tgatcaaaac catcaggatc ataaatggcg ttgaccggaa aatattactg atgatcgata
    19081 cgattccata aatgattcga gcatacggtt tgtttttgcg tccaatagag taaaggatca
    19141 atcccaaaac taatccgatg atcgtcacta agaccattga gattaatgtc atatatagtg
    19201 tatcaaggat agcctgattc atcgtttccg ggttgatttg tttaaaatct agatatgttt
    19261 ctgaaaagct tttatccacg atggatcacc tcaattccta ctccttgttc tttgatgaac
    19321 acttctgctt gatctaattc tttggtttca ccagtcatca agacagtcaa tgaaccaaag
    19381 gaaccttctt tcacttgttt gatctttcca tacacaacgt tgacatcgat gttatagcgt
    19441 cgaatcgctt gggaaatgac tggctcgttg gcatttcttt cgctaaaggt caaagttgcc
    19501 aaacggccgc ttggattttc tttgatgagt tcagctaata gttctgtcga atcagcttct
    19561 ggttcgatgt cttgttgtac aaagcgtttg gttacttctt ttttcggttg tctgaagaca
    19621 gtcaatacgt ctccttcttc tacgactttc cccaactcca tcacagcgac tttgttgcag
    19681 attttgcgga tcacattcat ttcatgtgtg atcaagacga tggtcaaatt caatttctta
    19741 ttgatcgcta acaataagtc taatacttct tctgttgttt gcgggtccaa tgcgctagta
    19801 gcctcatcac aaagcaggat ctcaggatca tttgccaatg ctcgtgcgat accgacgcgt
    19861 tgtttctgtc cgccggaaag ttgtgaagga tatgcttgtc cgcgtccttc taacccgact
    19921 aaacgcaaca gttcttctgc ccgttcttta cgtttgctct ttggtacgcc agctaattcc
    19981 agcggcagct ggatattttc aagtacagtt cttgaccata acaggttgaa atgctggaag
    20041 atcataccga ttttctttcg gaatgtacgt aattctttgt tttttaattt tgtaatatct
    20101 tgatcgttta cgatcacttc gccgctcgtg ggaagctcaa gtccattcag gagacggact
    20161 aatgtacttt ttcctgcgcc agaatagccg acgattccat aaatatcccc tttttcaacg
    20221 tttagtgaaa cgtcattgac ggcttggatg tttccgtttt tcccattgaa tgtctttttg
    20281 acattcgtta gttggattaa tgacatgcca ttcacatctt tctcttatga tttttcaaac
    20341 aaaaaagcct tcgccctctt gccttaagca aaaggacgaa agcttgaact tccgtgttac
    20401 caccttgatt taccgttact tcgcaataac agcctcttcc agtacttgga cttagcttct
    20461 ttccgtatac tgtcgcgctg taacgggcac tcccgtagta agtttgctta cgctcgctta
    20521 ctctgctcta agaccatctt ccatctcatt ttcgttaccc attttcagca aacagggctc
    20581 tcttcaaacg tccgtgacat gtactcttct tttcaaagct ttctcgtttc atataggatt
    20641 gcttatgatt atagcaactg gaagaatcag tgtcaacttt tttcaagtga ttttctgatt
    20701 ttataattcg tcaaattctt ttggatcaac atctttgaaa cttaagatcc acgcattcat
    20761 ttcatctgaa tcattcaaga tattgatatc ttcaccgatt ttatcattaa cagaagaaac
    20821 tgtacccgtt agcgggctgg caaattcatt gaccgctttt tccgcttcaa cttcgatcag
    20881 aggttcatct ttttttagcg attgtcctgc ttttggcaga ttggcaaacg tgatgtctcc
    20941 aagttcttct tgcgcttcat ttgtcaaacc aacacagtat tcttttccat taaataagat
    21001 ccaaaggttg tcctttttct tcaaatgctt gcctgccatt ttatatcagt tctccttcat
    21061 aaattttttc attgaatcca attgcaacaa gctggtcctc tcgtaagatc agtggtcgct
    21121 tgatcagcat accatctgtc gataagactt ggctcgcttt tgaagccgta aactcgccta
    21181 cttgatcttt cagtccaagt tcccgatatt tcatcccgct tgtgttgaac aggcgacgga
    21241 ttggtacttg actcgtttct agccaagtct caatcgttga ggccttcggt acttctttga
    21301 tcatatcaac ggtttgataa ctaacgccat gctggtctaa ccacgctttg gcttttttgc
    21361 atgttgaaca tttcgggtac cagtaaaagg tgtacaattt tcttacctcc ttgaagatct
    21421 tgcaggtgct tcattttggt atctcatcga taatatcaac ccgataccga tcatgttccc
    21481 taaaatcgag gatcctccct gactaatgaa aggcaatggg atccctgtta aaggaagtaa
    21541 tccgatattt gctccaatat tctcaaagac atggaacagc agcatcatga tcaaaccgct
    21601 tgcgatatag gcgtaaaatt cattattcgt atcgaaacac acccgaatca tacgatagat
    21661 taaaataaaa tacaagaaaa tgacgaatgc accaccgata aagccgaagt tttcaccgat
    21721 aacggtaaaa atcatatcgg actcacgaac aggcacatag atattgctta cgttgaatcc
    21781 tttaccaaac aagccaccgg taccaattgc caaaagtcct tgggccggct gataactcat
    21841 tcctgacgta tcatggaaag ggtccagcca tgaatcaata cgtgcaaact gataagattt
    21901 gaatccaact ttataaagca agtctcttcc gccttcagtc gtaaccaaga agatcgttcc
    21961 tgctccaacg actactgcca agattactac aggtgcgatg atgcgccaag agattccaga
    22021 catcaggaaa atcccgccaa agatcgcaag aaagacaagc atcgttccaa agtcatcttg
    22081 ggccatgatc aatgctaaaa ccggtattgt cacgatcaag agcttcccaa tcagccaaaa
    22141 gtccgatttc atcgtccgtt cttttacttg cgtattatgt ttagttacga tcagtgccat
    22201 catcaagatg tacgcaatct tcatcaactc tgctggctgg aaagtaaacg caccgaatcg
    22261 aaaccagttt ttagaccctg ttgaagcagc taaattctga tcatagaatt tgagcaagag
    22321 tcccattacc actaacccca aaccataaag gtaaggagtc agtttccaga tccatttgga
    22381 attcaaatgc atgatgacaa cgattgctgc cgctccaact aaataccaga caacttgcat
    22441 ccctacgcct ttcaagactg ctggattgtt cggatcatgg ctaagtgcta catataatga
    22501 gagcagacca atcaaacata acagaaagac tggtaaaatc acgccataat caatacgatt
    22561 atcgttcgaa gcttttaatt ttttttccat gtttcatccc ttttcttaaa aaccatactg
    22621 ctgattatta tagcggtcca gttagataat ggaaagtctt tccatagaca tttgggaaaa
    22681 attaactttc cgtttgtgca gttgcttgag caaatgcagt acaaaagcct actgcttatc
    22741 tttagtgtat catagaactt tttttgatac atgcaattat ctaccagctg agattctcct
    22801 cgctgtgaga agtttctgtg ctttcattgt atcgaaggat ctcttcggca atctctagca
    22861 gctgatcaga aaccagattg gttcgttgga tgaaataaaa atatcgatgg aaattcaacg
    22921 gagtcattgg cacattttct ggaacagagc gcttagacaa aatcgagcga ccgattcctt
    22981 ccttcagcaa cgcaacaatt acatcgttac ttttcacgat catcttctta tcttggatat
    23041 tcatctcttc aaaataacgc tgcgtgtaat gaaaaacacc agagtttgct tctcttacaa
    23101 gccacaattt gctttttggg tccccagcga tgactagctg gtcttccatc aaagagtatc
    23161 tttgtacgcc ggaagtctct aatggctttt cgataaaacc aaaatcggat tcatgtttcg
    23221 ctacttgttc taatgattca tgagaattcg tgatttcgat ctcaaaatgc actttaggaa
    23281 accttttgat cagatgtttc atcaactgag gcaaaagata gacagcaaag gtgtgggaag
    23341 caacgatccg acatacttct tcttcatttt gttcattgag caatttttgc tgagtttctt
    23401 cccattcttc taacaaaacc agtgtccgtt catataataa gtcggcttgc ttagttggaa
    23461 gcatctcttt tcttccgtta cgttgaaaaa gatcgatatt caattcccgc tccaactgtt
    23521 tgatttgatt cgagactgct ggttgagata aaaacagtac ttctgctgct ctggaaaaat
    23581 ttcttgtttc atatactgct cgaaaagtcg ttaataactt aaacatggca ccacttccat
    23641 aactttttat gataattact ataaaaacta tttgatttaa atatacaaaa aaccatctat
    23701 aatagaaatg tacaacaaaa cagttgctga tccaactagt aacttagatg aagacaatca
    23761 gctgtctgct tgctcgtaat catacattcc tattcatttt acaatgtatt cgttcatctt
    23821 ggtactaaaa tctattaaat aaaaaggaga atattatata tgaagaactt tttgaaaatt
    23881 attccgatcc cgatctgcgg gttgatcctt gggttagctt cccttggaaa tctgtttaaa
    23941 gactatcacc atgttgcttt aggcaatcta actggcggta tctctatgat tcttatgata
    24001 ttcatttttg ttaaattgtt tatcttattc gaacacacga aacaaacgtt gcatgatcca
    24061 atcatcgcct ccgtttcacc aacttttacg atgtcactca tggttatttg tacttatttt
    24121 gtttcatatg aaagtattgc accaattgtc aaattcattt ggctgtgcgc tgttatcttc
    24181 caagtgatcc ttgtctttta tttcaattat caccatgtta tcaaagctga tctttcaatt
    24241 gaagcaattt atccaagctg gtttatcatt tacgttggat ttggggtcat cactgtaact
    24301 gccgggaact tcttcccact tattggaaaa attttctttt ggatttcatt gatctgctat
    24361 gcgttcttat tgccgatcat catcaaacgt gtctttttcg taagaaatat ggctcatccg
    24421 acacgtccgc tgattacgat cgttgcagca cctggttctc tttgcctaac aggttatctt
    24481 aaaaactttg ctgaacctaa tttttatctt gtcgtagcat tgttcgcttt atctcaagta
    24541 ttgtatttcg ttgtgctgtc tatgcttcca cgtttgttca agcttcgttt ttatccaagc
    24601 tatgcagcct ttacattccc gcttgttatc tctgcaacag ctatatttac tactgttcat
    24661 tacttcaaca gtttaggtat ctatagtagc gtattggatt tcttaaaagt tgcggaatgt
    24721 ctgtttgcaa gtgtagttgt cttctacgtt cttgctcatt acgtacgttt cttaattaaa
    24781 gaacatagca aacatggaaa atttgtttct aaaaaaatcg cttaataatc gattcaatag
    24841 gttgtgaaaa agctgttctg catcaagtaa taagaacggc caaacaaaaa cagctgttca
    24901 tgtttttgta ctggttgggc ttattaccgc agatgcaagc ttttgaacac cgtttaccaa
    24961 gaggtcgtaa aagaagtggt cagcttcaag aaataagaag gaatttacga aaattgcttc
    25021 tcaaattttt gtgaaatttc ggcttatttc cgtagaagct acttctggaa caccgtttat
    25081 tcgaaacaag aggtcgtaca agacttttgt cacgacctct tgttcttttg tcaaaatact
    25141 ggtttagtgc tactatgttc gagtatgctt ttagtattta ttgacgaaag gaaccgatca
    25201 aatgaaatca ttgtcacatc caataaaatc tgtaaaggat tcagcggtcc gcgaaatctt
    25261 acccggttta ctgctttctt ttgccatcgc tgccttcagc aggcttcttg ctatttatct
    25321 gccacagtta ggtggagcga ctattgctat tttattgggt attttattag gaaatacttt
    25381 ttttaaacag cctgttctag caatcggcac aaaatttgct gaaagcaaac tattagaatt
    25441 ctctgttttt ttactaggtg caactgtcac agctcaaacg attgctcaaa ttggtgtaaa
    25501 aggaattgtt tttgtgttac tgcaaatgtc agtcactatc atcggtgctt acttgattgg
    25561 taaaaaactg caattcagcg atacttcttc tttgatgatg gctggaggaa acgctgtttg
    25621 tggttcttct gctattgctt caatcgcccc ttccatccat gcgaaagagg aagaaaaagg
    25681 acagatcatc acactagtca atctactggg tactgtgatg atgttgactc tcccttttct
    25741 tggattacag ttgtttggtg gaaatctcat tgaaaaaagt gcacttttgg gtggtacact
    25801 ccaatctgtt ggtcaagtag tcgctggggc aagcttactt gacaattctg ttgtcgaatt
    25861 cgccatgcta tttaagattc tacgtattat gatgctcgtt gttgttgtct atctgtttga
    25921 aaaattcatg cacaagaaac aaccagctct gtttgaaacg ccaactgata cgcagaaaaa
    25981 acgaaaatta ccgcttcctt ggtatgtatt agggtttgtg atcttttgtt tggtcaacag
    26041 cttgttttct ttacctgtag ttattgacca atcggcacat tttatcagca gctggtttga
    26101 gatcactgct ttagcggcaa ttggtctgcg tttagacttt gcaaaattca tgaaagaagg
    26161 tacacgcttc ttatcttatg gattagctgt cgggcttctc caaacagtga tggctttact
    26221 atttatttgg ctgatcaaag tttagaatga acatgaaaaa acagttgtga ttctcgcatg
    26281 gaacaatgtc cgaatcacaa ctgtttttaa ttttgcagat atccttctaa caacaacaat
    26341 tggcgtttga tttcaacccc atctattgcg tttcccatat atcctgtcag ttttccattt
    26401 tgcccgatca ctcgatgaca aggaataacg attggcaatg gattggttcg atttgcctgc
    26461 ccaatcgctc gcacagcttt cggactatca acagcaacgg ctacttcttt atacgttcgg
    26521 atttctccat aaggaatggt cgacaaagct ttccagacat tttgctgaaa aggcgtacct
    26581 ctctttatag ataaaggaac atcaaattct tttctttttc ccgcaaaata ttcttcaaac
    26641 tgttcttgtg tgcgcgttaa aattcgctga ttcgctgatt cgctttctga aattggctga
    26701 aaagatacag cagtcaatcc ggattcatct gcatctaacc ataaaaagcc gataggtgtg
    26761 tgtattttca ttttgtttgc tcctagtcaa tagactggaa cttcctcctt tttctctatc
    26821 caaaactact tgccaactaa taaaaaatgt tcttaaaagt ttgataaacc gaggaaacat
    26881 ggtaaactaa atacgttagt ttacaaaaag aatacctgtt cttttacgta aaatcaaatt
    26941 ttaaaggtgt tatttaaggt ttattaaatt ttgaatgtaa accatatata aacatacgat
    27001 tgaaaggata gaaacgatga aagtatcaat tgttgtccca tgttttaatg aagaaaaaac
    27061 tgttcctctt ttttttgatg aggttgaaaa attcaaaggt gcccatgagt tcgaatattt
    27121 gtttattgac gacggatcaa aagacggtac gttagcagca atcaagaagc tagcctcagc
    27181 ttatccttat gtccgctttg tctcattttc cagaaatttt gggaaagaag cggctctgta
    27241 cgctggattg caagcttcta caggagattt agtgaccgtg atggatgttg atctgcaaga
    27301 tccgcccgaa ctgcttcctg agatgatcaa ccggatcact acttctgata tcgattgtgt
    27361 cggcacccgg cgttcgactc gtgaaggcga gcctgcgata cgcagtttct ttgcgaaaaa
    27421 attctaccag ttgatcaata aaatcagcga tacagaaatg gtcgatggcg ctcgtgattt
    27481 ccgcttgatg actcgtcaaa tggtggatgc cgtcctagaa ttaacagaat acaaccgatt
    27541 ttcaaaagga ttatttagct gggtcggatt taaaacagaa tatatttctt atgaaaaccg
    27601 cgaacgtgtg gctggcgaaa catcttggtc cttctggaaa ttattcaact actcgattga
    27661 tggtatcgtg aacttctcgg atgcaccgtt aacgattgct tcatttatcg gcgctttgtc
    27721 ttgtgtcggt tctggtattg caattttgtt cattattttg cgggcattgt tgtttggtga
    27781 tcctacatca ggatggcctt ccatggtctc gatcttcctg ttcattggtg gaatccaatt
    27841 gttatgtctt ggaatcatcg ggaaatatat tgggaaaatc ttcttagaaa cgaaacagcg
    27901 tcctgtttat atcataagag aaacagaaaa agtgaagcca aatgaattga agcctcacca
    27961 ataatatacg tcaaatgaag taaacatttt tttaaatgaa aaaggactgt gacaaaagtc
    28021 ttgtcacagt ccttttttcc gaataaatgg tgttccagaa gtagcttctt cggaaataag
    28081 ccgaaatttc acaaaaattt gagaagcaat tttcgtaaat tccttcttat ttcttgaagc
    28141 tgaccacttc tatcacaacc tctttcatat cttattactt cttcattgct gcttttagtg
    28201 ctgctgctaa tgggctttct tctggttctt ctgcttgtgc atattttttc agcaagcgtc
    28261 gttcttcatg tttggtcatt ttcttcttct gctctttttt atcaagcaat ttttctgtcg
    28321 ttccatcgta tttacaacgg aaataagcac cgtttttacc ttcgatgatt tccattttcc
    28381 ggtgacattg cgggcatcga tgattagaga cttttggatc ttttcttctg cggtagttgc
    28441 aatctgaatt ggtgcagaca tagattttgc cgtcacgtgt gtttttctcg cgcagattgg
    28501 ccccacattc tgggcatttc ttttgggtaa tcgagaaatc ttgatattct tgtttgcttg
    28561 atttgatctc attcaccaag cgttttgttt ctttttcgat tccattcaag aaggttcctg
    28621 cttgtttctt tcctgcagca atagcttcaa ggtctttttc ccagctttct gtcaaatctg
    28681 gtgttactaa agagggattt accaatacta ataattgctt acctttagga gatacttgca
    28741 gtccttgagg ggtacgttcc atcagttcag atttgattag tttctcgata atctctgcac
    28801 gcgtagctgg ggttcccaga ctgtattttt ccatttgacc aagcaagctt gcttctgaca
    28861 atggtttagg cggtgcagat agttcttttt tgatggcgaa atcgggattg attctcattc
    28921 ctttttccca tttgactgtt tccgtctcag tttcagtttc gcctttccat cctgcttgga
    28981 cgacacgatt ttgccgaaaa atgaatcgtt ctttaccaaa ttcaaccgta acttttgttt
    29041 ggctgacttt atggggttga gcaaacagtc ctaaaaaacg agagacaatc atttgataga
    29101 tttttcgctc ttcattactt aatttttcat aacgtgctct gttttcagtt ggcaaaagtg
    29161 catggtgatc ggtcacttta ctgtcttgga atacttttgt ctgttggacg atggctccgt
    29221 ttttcaaata ctgcttgacc tcaggagcaa agtcgctgac tgcttgcaat cgttctttca
    29281 tcgtactttt catgtcattt gtcagatatt tgctgtctgt acgcggataa gtaacgatct
    29341 tatgggtctc atacaaactt tgtacaagac ttaatgtttt ctttgcagaa tagccatagc
    29401 gttgattggc ttctctttgg atctctgtta aatcataagg caatggcgca gattccgttt
    29461 ttaatttttc ttggacttct gtgacagttc ctttgcttgt actgatcaat tgaacaagtt
    29521 tttgtgcttc ttcttgtgtt tttaaagcaa attgattttt ttgaagcatt ttggcttgtt
    29581 cttttccagc ttgcaacgtg atcgtgaaat acgtttgtgg acggaactgc tcgattttct
    29641 tttcttggtc tctcactaat gctagtgtcg gtgtctgcac acggcctgct gataaattat
    29701 ccgcgtactt cacagttaag gctctggtca cattcaatcc taccagccag tccgcttttg
    29761 cccgggcaag agcagattca tacaatgcat catattcttt cgcaggtctt agttgcttga
    29821 atccagtttt gattgcttta tctgtttgag aagagatcca taaccgcttc actggtttat
    29881 tgaaatgcac atactctaag atccagcgag caacaagttc cccttctctt ccggcatctg
    29941 tcgcaatgac tgcttctgag acatcttttc gctgagccag ctgcttgatt gcttttaatt
    30001 gatgtcctgt tttaggaagt ggtttgattc ctagtttttt agggatcatt ggcaacgtgt
    30061 ccatttgcca gctctgccat tccttattca gatcttccgg cattttcaat cctaataaat
    30121 gaccaagggc ccaagtaacg atcaccttgg gtccttcata ataatttttg tgcttttgat
    30181 tagcaccaag caccttgctt aggtctttcg caacgcttgg tttttccgct agaattagtt
    30241 gtttcataaa attcctttct gctttctact gtgatgggct gatgattttt caacaacgca
    30301 agtcccttgt attgctgtaa atcatcgtca cactcttcac accagcgctc atctccgtca
    30361 ttccaaaagg cgctgagaaa gttacttttt gcattgatat attcataatt ttgatggatt
    30421 tcgttccatt gtttttcgta cgcttgtcgt gcttcttcga aagactcgaa tgtttcttct
    30481 tcttcaatat cttcttccca gtcttcaaaa aaccaccaag gctcattgtc cccgaacatg
    30541 gtaattactt gatacatact cgtgctcctt ctttcagctt ttctagactc atcttagtca
    30601 cttttcaaga aagttgcaag aattttctct tataaaaggg tcatttaatg aacatcttaa
    30661 aattgttaca aatgaatctg attgtttaag gcaaagtagc gataaagtta ttgatcatcc
    30721 aaaaatagct gtcttctgga tcgattgttg gttcttttgt aaagtatcct aaaaaacgta
    30781 aggaaagata accgtggatc atactacgaa gttccctgct tcgatgaact ttttcttctt
    30841 tcgttaattg aaaagcctca aatatctgca aaatgatctg attcgtttca tggattccat
    30901 ctaataaaac agcttcattg gtatgcggta cactgatcag taactcatac actgcttgat
    30961 ttgaaaaagc aaaggatcga taagtttctg catagatctg cagtgcttcc tgaccacttt
    31021 ttcccacaag agcccgtcgc agctggtcat taagttcctg cagaagtttc gctgttagcg
    31081 ctgttttgac atcacgaata tttttgaaat ggttatacag cgagggagcc ttgatatcca
    31141 agcgtttggc taaatgctga aaggttattt gctgaacatc catctcttct gctagttctc
    31201 gaaagcagtc gatgattttc tcttgagaca gatttctttt tttcatttcc atgtttcctc
    31261 tcataaaata aatccttcac tctttcagtt taccacgaaa aagaaagaga aaaaagagac
    31321 tttcattgat aaactacata ttgtagttta taataaagag ctgtagatga aaattttcag
    31381 aaagaaggag tctaattggc tgcaagaata aaggaaaaat tatttaggaa aagagaaact
    31441 tgggaacaaa acttgtttgt cttatggttt ggaacattta tggctggtat cggatttagt
    31501 ttagtcatgc cttttatgtc actttacata gatacgttag gtaattatag tacgtcacaa
    31561 ttgaactttt ggagcggatt gactttttca tctactttct tggtgactac gcttatctct
    31621 ccttggtggg gaaaattagc cgaccaaaaa ggaagaaaat tgatgctgtt gcgtgcttca
    31681 ttaggaatgg caatcgtcat ttctttgatg ggttgcgtga ctagtgtgta tcaattagtc
    31741 gcattgcgtc tgcttcaagg gattttttct ggttatatca gtaatgccac tgcccttgtc
    31801 gcaactggga cgccaaaaga aaaaagtggt caagttcttg gaaccttagc aactggttct
    31861 gttacaggaa cattacttgg accgcttctt ggcgggatct ctgcttcagc gtttggctat
    31921 cgccctacct ttttcattac agggatcatc ttatttttcg tcttcttgtt gagtctgttt
    31981 ttcgtccatg aaaaatttgt cccagtgaaa aaagaaaaca tggtttctgc caaacagatt
    32041 ttcaaagact tgaaatatcc acacgtcgtc atcggtatgt tcgtcacaac gatgatcatc
    32101 caagcatcca acaattccat tagtccaatc atcagtctgt acattcgcca attgctgcat
    32161 ggacatggga atgttacgtt gatcagcgga atcattgctt cgatacctgg gattgccact
    32221 ttgatagccg ctccaagatt tggtcgctta ggtgacaaga ttgggagtga aagaatactt
    32281 gcgattggtt tgggttttgc gattctggtt tatatcccaa tggcattcgt cacgaatgtt
    32341 tggcaattag ctgccttacg ttttttgatt ggtatctctg atgcttgtct gctccctgct
    32401 gtccaagcat tgatcacaaa atattcgcct catcaagctg ctggacggat cttcagttat
    32461 aaccaatctt tccaagcaac tggtaacgta gtagggccta tgatcggctc cagtgtttcc
    32521 agtgcatttg gttatcgtgg cgtctttctt tcgacttctc ttcttgtttt ggtcaacttc
    32581 ttgcttgtac gacacaatac acaagagatc catcagcaag aaactgcaga agaaaaagct
    32641 tcaggagtct ccatagcttc agggcactct ccttctcata gacaataagc gtaaatgaaa
    32701 aacactgctt gaacagttgt cacagactgt ccttgcagtg tttttgttta ggcctgtttt
    32761 ttcaagaact cagataagag gaataatccc ttttccaagg tttctttttt acaacagtaa
    32821 cctagtcggg catgcttcgg taaatcaaag gcacttcctg gtactaatag tactcctgtt
    32881 tcttctaata aattgataca aaatgtctgg tcatcttctt cgatggctaa tttaatgaaa
    32941 gaagtagaga cgtaattggg agcgacaagt tcaactttcg gttcattggc tacccattgt
    33001 tttaaaatca caaggttctc tgtaatgatc tttttgtttc gcgccaatat tttttcttta
    33061 ttcttcaatg catgtacagc aagatcatct gacaggactc cgccgcaaat catcgtgtag
    33121 tctcgatact ttcgaaaaac ttctgccaac tcaggtccag tagctgtcca gccaatacga
    33181 atacctggaa tggagtaggt tttggatagg gaattggttg ctataccttt ttcatagaca
    33241 tctacgatcg acaagaattc acctttatcc gttaaaggcg catatacttc atctatcaat
    33301 acatatgcat ctactgtacg agcaatttct gcgatttttt ctaaagtttt ccgatctagc
    33361 aatgtgcctg taggattgtt tgcactattc aaacagatga tttttgtttc aggtttgact
    33421 aaggcttcta gctgttctaa atcaaactgc caatcttctt cttccttcaa atgaacaaag
    33481 tcaacagcgg cacctaaaga tttggggatg tcatataatt gctgataagt aggaagcatc
    33541 gaaacaacat ggtctcctgc ttccactaac gcgtatagag ctaaaagatt agcaccagtc
    33601 gctccattcg tttgtaagat gttttccgga tcgacggttt gataaagaga agcgacttct
    33661 tgtttgaaaa catctgatcc ttcgatccag ccatagtcca atggttcgtt tttgcgtttt
    33721 tcgaaaaatt tacttacgct agtcccatct aagccaacga gttcgtctaa tgtcaatgct
    33781 tcgatcgagc tttgtgcaat atcatatacc gctttctttt cccatttatt tagccattct
    33841 tctacaccga aatttgcgat tttcatgttt ctttccccct tatatcttga tccagcctac
    33901 agcaagcaac ccgattgaaa gcagtgcggc cacacaaatc agcccaatca ttactttttc
    33961 ttttcccgta atttgatgtt tggtttcacg acaagcgatc cagtagaagc aaaaaccagg
    34021 aagataggca atactacata aaaggacata agaaaatcct gctaaaatga tgcccatcaa
    34081 ttcaaaaact acagcaatcc caccgatcac tacttgtttt ttgattcctt cttttaaaga
    34141 aaactggact tgatagatcc cgacaagtag ataacaaatc aaaattgccg ctgtacataa
    34201 agaataggca aattgatagg cataatcagt aactaagaaa gtcaataaga atagattagt
    34261 caagattgca gtaacaacta acgaataagt tggggcattt tttttattcg tttttcccca
    34321 acctttcggc aacagaccgt ctcgagccat caacatcgtt gtttctgctg ggagcatcgt
    34381 ccaagacaac caagaaccaa tgatcgaaat aatcaagccg atattgatca gcgctgctcc
    34441 ccaatttccc acgattcctt ttaaaatatt tgccatagca ggttgtgaaa tagttgctaa
    34501 ctcttcttga gataagacgc cgtatggcaa gatcgaagca aggatataga taaacaataa
    34561 acaaagcaga ccaatgatcg atgcttgctg ggcttctgaa cgtttcttcg cgcggttggc
    34621 taaaacactt gcgccttcga tccctacaaa gacccacatc ataaccatca aacaaccttt
    34681 aatctgcaac cagatcgttc cttgctgccc tgttccattc actagattat cagatacgtt
    34741 accccaaaaa tcagcagtga atatattcac tttaaatgcg ataaatacaa atattaaaaa
    34801 taggaataat ggcactaatt tacagatcgt cacgattgta ttgatgacgc tcgctccttc
    34861 aactccattg tttactaata aagtcagacc ccaaataaaa atacttgcaa ataaaataga
    34921 aggaacattt tgtccacctt tgaatatcgg gaaaaattct cccatggcgc tcatcagcat
    34981 cgttgcaaaa gcaacatttc ccagccacgc agacaaccag taaccccaac cactgatgaa
    35041 gccacctaat ttaccaaatg aagcttccgc gtaaccgaaa ataccaccat ctagatctgg
    35101 acgcttttct cctaaatgat tcagcgataa taccaaagct aaaataccaa tcccaacgat
    35161 tatccatgaa aggatcgctg gccctggagc tgcagaacta gccagatcac ttgtgatccc
    35221 aaacacaccg cttccgattg acgaactgac aaccagagca accagtcccc atttgctaat
    35281 acccgtcgat ttcttttcca tttccatcac cctcacttat aaaattgaac aaatcacttg
    35341 caaaagattc ccttctattt tgcaactgct cttgcttaac aaaaagcaac tttattcata
    35401 ttattggata ttatgaactt tttacacaag tatttcaagt ataaaatgaa taaaaattaa
    35461 ttttattgca aaaaaacagc tatgctatcc aaaagaaaag gtgtttcttt tgttagccat
    35521 agctgttttg attcatttaa tatcgcttaa acgtattgta aaacttttgt ttcttgatat
    35581 gcgtggtcga tcaaaccacc acccagacat tccattccat cataaaatac aactgcttgt
    35641 ccaggagtga ttgcacgtac aggttcgtcg aatacgactt ctgcacgatt atcatccaat
    35701 aagcgaactg tcacaggaac gtcttgctga cgataacgga atttcgctgt acacttgaat
    35761 tcttttggca ttggttcatt cgtcgtaaag tggatttcac ttgcatccaa gcttgtggca
    35821 tacaatgcag gatgatggaa cccttgcccc acatataatg tgtttgtcgc tagatctttt
    35881 cctacaacaa accagggttc ttgcgaatct cctccgccac cgatccctag tccttgacgt
    35941 tgaccaatcg tataatacat caagccagca tgttgtcctt tgacttcacc atcaagagtg
    36001 accatatttc ctttttttgc tggcagatag ttgctcaaaa attgtttgaa gttcttttca
    36061 ccaataaagc agattcccgt tgaatctttt ttcttagcag tcgcaaggcc tgcgcgttca
    36121 gcaattgccc gtacttctga tttttccatg ccgcctagag ggaacatcgt ttttgaaagc
    36181 tgttcttgtg ataattggct aaggaagtaa gtttgatctt tattattgtc tactccacga
    36241 agcatgtgga ccacgccgtt ttcatcgcga gtgacttgcg cataatgtcc agtagctaca
    36301 taatcggcgc caagatccat tgcataatcc aagaatgctt tgaatttgat ttctttgttg
    36361 cacatcacgt ctgggttagg tgtacgtcct gcgcggtatt cggctaggaa atattcaaat
    36421 acacgatccc aatattcttt ttcaaaattc acagagtaat aaggaatccc gatttgagca
    36481 gctactttcg ctacatcctt ataatcttcc gtggccgtgc atacaccgtt ttcgtccgtg
    36541 tcatcccaat ttttcatgaa tattccgatc acgtcgtatc cttgttcctt taacaaaagt
    36601 gctgttacag atgagtcgac accgccgctc atccctacga caacgcgtgt tttgctgttg
    36661 tctgtcatga tatcaccatc atttcaatta gattctgaaa catctacgag ttgaaggtcg
    36721 caacttttca gtattttaca ttatacgtca tatttctgaa aatttcatcc cttaattatc
    36781 aagtcgtaaa aatactgttc tactggacct attcagtaca caaacatgat agtcaaccaa
    36841 aaaatcactc atcttttgag cattcgatat aaaaaattgg catactcata aaaagggcca
    36901 gcctttcggc aataaggaat aattttataa attgtgaaaa actatttttc aaaattgctt
    36961 cttatcgctt aaagctgcac gtctcattta caaccttttc tttttagatg ttatgagttg
    37021 ctcagataag tgattttgtt tactctaaga aaaaagggag gcaaaagata catgaattac
    37081 tttatcagtg atatgcactt tttccatgaa cgcttattag gaaaaaacga ctttgctccg
    37141 cggctctttg caaatgtgga tgaaatgaac caacagttga tcactagctg gaatgcagta
    37201 ataaaagaaa ccgaccatgt ttatcatcta ggcgaccttg ctatgcatcc gcaatatgaa
    37261 aaagcaaatg cagaaatttt ggaactggtc actcaactgc aaggaacaat ccacttgatc
    37321 aaagggaacc atgacagtcg cgcttttttc cgttatttag aaaaaaatga tcctaggctt
    37381 actgacggaa gtcccaaatt ttatttttat gatgtaggtg ccatcgttaa attcgaccat
    37441 caacaatatt acttgactca ttatccgatg ttgctcgggc ctaacgacac tatccgcaat
    37501 cttcatggac atatccatca ttacagcgta cctatcgaga acgatgtcaa tgtaggcgta
    37561 gacgcaccgg aaagagaact cttaaaggaa aaactaccat ttggcactcc gttatctgag
    37621 caacaaatca aagaaatcta tgaagcaaag aggaagtacg ataggtcgtg aaaaaactgc
    37681 cctatatcaa gtattaaaca aataaagata attactcata tttttcattt gagtaggctt
    37741 attgccacag gaacattagg atcagaaatg gagttgtaac aagacttctg tcacaactcc
    37801 attccatact attcgtcttt ttgctcatgg tgttctttta ccagtttttt ctgtaattct
    37861 ttatcataat ttcgggcagc tgacgttaaa aataaaacga ttccgcataa taaaatcccg
    37921 attccggaaa acaagaacat tttttctacc cctaaacgat cagctaatgg tcctgcaaat
    37981 aataatccaa ttggacctgt caggttcatc aaagagttga agttccctaa gacacgacct
    38041 aaaacattcg attcataact ctgctggatc attgccatca gcagtgtatt gaaatatggc
    38101 gttgcacaac ctgctcctgc attcaagata agaaagtaca agaacccttg actatcgttt
    38161 ggaaccaatc cgcttgcacc gatcgtcagg cccataatga tgtaggctat aataataggt
    38221 ttcattcggt ctttccatgt tcctataaaa ctgatcagtg caccgcctaa gagcattccg
    38281 actgcgtaga ctacttccac tgctcccgcc tgtccaacgg aaccaccaaa gtaatctagc
    38341 gtcatcaatg gatacagact gattgctggc atgaacaaca gcatcacaaa tgcaccattg
    38401 atcgttatgt accataaccc tttattatcc atcaattgct gcagcccaaa ttttgcatct
    38461 ttaaaaatag taagcggaac ttccaatatt tcaggagaaa cttttgggat cttcacaaaa
    38521 atcaataagc cgacaccaaa aactgccccc aatacatcta ataaaataag gtaattgaca
    38581 ggaacgactg aaaacaaagc cgcacctaga gctggcgcaa tgatgaaatt cgctgattgg
    38641 accattccta actgtccatt cgtccttgtt atgtgagaag atggaacgat tgtcggcata
    38701 atcgactgga tcgttggcat ttggaatgtt tgtgcgaccg aacgcataaa caaagacaca
    38761 aaaactagcc aaatcgggaa ggattctgaa atcgttccga ctactgctaa aataagcgcg
    38821 aaaatcgcta caataatatc cgtgacaatc aaaagagctt ttttatccca gcgatccact
    38881 agcggtccta caaatgggct tagtaaaacc atgggaatca ttcctagtaa agtagcaaaa
    38941 ctcaaaattg tcgccgaacc ggtttctctt gtcaaatacc agatgatcgc atattggacg
    39001 accatactag tgattcctga caaaaactgt cccgataaaa agaacaaata atttctcttc
    39061 caattcgtta aaacgaccga ctgttcattt tccataaaca tccctctttt ctttttcatt
    39121 ttgtatagcg tttgcacaac aatcattata cgggattctt ttttcaaaaa ataccttttt
    39181 ccctaaataa caatcgtttc aatttaaatt tctttactta gaaacaataa atcagcaaaa
    39241 tattaaaaat aacaacatgt gttaaattta aaaataaaaa agacaactaa tttgcgtcgt
    39301 ccttacaaag ttagtcatct aacaataggc cgtttcaaaa ctagttgtct ttttatatcc
    39361 acagatcatt cgactttttc gaataaccca cggctaatca tcccatccat cagaaaataa
    39421 aatttatctt ggctcatttt ctgttttgga tctttgaaaa tattgactgc tttcaatcca
    39481 tttgctgtag tgatcgacat tttcaatgtt tgaatatctt ttgatacagt gatttttaat
    39541 ttgaatcctt ctttgctttg cggcgtggca tctaatggtc ggatcaattg ataattgcct
    39601 ccgtttgttt ctgtaaatcc attatcgcgc aatgtatatc tttttgcatc aggatgcaaa
    39661 cgatagctaa ttggtgcgcc cagtaccgtt gctgttgttt caaatgccat gattttcacc
    39721 tcgataaaat tagtttctct ttattataaa agtttatcct gatttttact aggtttttca
    39781 gttaagttcc ctaaaaaatg aaggcgttct ctcagacatc tgttcatata agaatgactt
    39841 cactagttat tttttgacag cacgaactaa ttgatcaagg aaatactgga tgtcgtcttc
    39901 ggtatttccc aaaccaaagc tgatacggat ggattctttt agtatcggtg tatcttctcc
    39961 atacattgct tccaatacgt gtgaaggctc gatatttcct gctgtacaag ctgaaccagt
    40021 agaaatagcg accccattaa gatcaagctt catcagtagc aaatcatttg cttcgccaat
    40081 taagcgaaga ttcaacacat gagggagttt attcgttaaa tcaccattta cttgataagg
    40141 gatttcgttt gcttctaatg tatcaagaat caattgctgg aatcctttat atttgttgtt
    40201 acgtgcttca cgttcttcag gtgacagcag ttcaactgct tttgccatac cccagatccc
    40261 tgctaaattt tctgtacctg cacgccgctt ttcttcctgc tccccgccta aaagcaatgc
    40321 agggatattt actccgtctc gtttatataa gaaaccgacg ccttttggcc cattgatttt
    40381 atgagcagag atacttaata aatcgattcc taattcttct ggatggatcg tttggtttcc
    40441 gtacgcttgt accgcatctg tatggaaata agctggatga tccgctaaaa gttcaccgat
    40501 ttctttgatt ggcatcaaat ttccgatttc attgttcccg tacatcacag agaccaagat
    40561 cgtatcttct cgtaacgctt ttttgacctc ttcgatcgac agattccctt tttcatctac
    40621 aggcagatat gtaacgtcaa acccctttgt ctctaaatat ttcatggtat taagaacagc
    40681 aggatgttcg atagctgtag tgattagatg tctgccttct ttttgacgcg agaacgctgt
    40741 ttctaagata gctgtattat ccccttctgt tcccccactg ttaaagatga tctcgtgagg
    40801 ttttgcttgc agactgtctg caatgatttg tcttgctgtt tccaattttt catgcgcgtg
    40861 gcgaccaaat ccatgtatgc ttgaaggatt tccaaacgtg tcttgcatga ttgccgtcat
    40921 cgcttcaatt acttgtggat gcatgggggt agttgccgca tgatccagat aaatagtttt
    40981 cacttcattc atcccttcca aattattgct aggcacattc tagcataaaa tagcgaacaa
    41041 accaaagaag agctcatttt tgtctagagg acgtttcatg ggcatagaaa aaaggagctg
    41101 tgacaaaagt cttgtcgcag ctccttgaag ctgctcactt ctgtcacaac ttctt
//
LOCUS       DARBET010000019        40414 bp    DNA     linear   BCT 07-NOV-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4027
            SAMEA114569841-rid20112183.denovo.019, whole genome shotgun
            sequence.
ACCESSION   DARBET010000019 DARBET010000000
VERSION     DARBET010000019.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA114569841
            Sequence Read Archive: ERR12203666
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 40414)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 40414)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001972618.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001972618.1
            Genome Coverage       :: 132x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/07/2023 13:18:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,041
            CDSs (total)                      :: 2,975
            Genes (coding)                    :: 2,908
            CDSs (with protein)               :: 2,908
            Genes (RNA)                       :: 66
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 67
            CDSs (without protein)            :: 67
            Pseudo Genes (ambiguous residues) :: 0 of 67
            Pseudo Genes (frameshifted)       :: 26 of 67
            Pseudo Genes (incomplete)         :: 28 of 67
            Pseudo Genes (internal stop)      :: 20 of 67
            Pseudo Genes (multiple problems)  :: 6 of 67
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..40414
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA114569841-rid20112183.denovo.019"
                     /isolate="VRE4027"
                     /db_xref="taxon:1352"
                     /country="Denmark"
     gene            complement(57..1586)
                     /gene="ahpF"
                     /locus_tag="SCJ86_001086"
     CDS             complement(57..1586)
                     /gene="ahpF"
                     /locus_tag="SCJ86_001086"
                     /EC_number="1.8.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339249.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alkyl hydroperoxide reductase subunit F"
                     /protein_id="HEG1168664.1"
                     /translation="MLDKETKQNLEQYLALIESPIVFSVSLDNSENSQKLAEFTKEIA
                     EMSPKISWEKRDSMRTPSFSINPVGKESGIVFAGIPLGHEFSSFLLAMLQISGRAPKI
                     SESLSLKIKKITQTLHFDSYVSLTCHNCPDVVQALNILAVLNPNITHTMIEGGMFQKE
                     IDDKKIMAVPTVFLNGEEFSSGRMTIEQIVEKITGPLIEEELFEKEPYDVLVIGGGPA
                     ASSAAIYAARKGIRTGLVADSFGGQVVETLGIENMIGTSYTEGPKLMQQVESHVRSYP
                     IDIMMNQQAVSLNKEQHFINIGLANGISLKAKTAIIATGAHWRSINVPGEKEFKNKGI
                     AYCPHCDGPLFKGKEIAVIGGGNSGVEAAIDLAGLAKHVYVLEFLPELKADQILQDKL
                     VSLANVTVIKHAATKEISGTNQVESLSYIDRRTMEMHTLAVSGVFILVGLLPNTDWLD
                     GTIEMNPRNEIVTEKNGATNIPGVFAAGDCTDSPYKQIIVSMGSGATAALGAFDYLMR
                     T"
     gene            complement(1596..2159)
                     /gene="ahpC"
                     /locus_tag="SCJ86_001087"
     CDS             complement(1596..2159)
                     /gene="ahpC"
                     /locus_tag="SCJ86_001087"
                     /EC_number="1.11.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309198.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peroxiredoxin"
                     /protein_id="HEG1168665.1"
                     /translation="MSLIGKKIEEFNAHAYHQGEFLEVSEKDLMGNWSILCFYPADFT
                     FVCPTELEDLQEQYSTLKSLGVEVFSCSTDTHFTHKAWHDTSDAIGKIEYVMIGDPSH
                     QISRIFDVLDEEQGLAQRGTFIIDPDGIVQAMEINADGIGRDASALIDKIRAAQYVRT
                     HPGEVCPAKWKESGETLKPSFDLVGKI"
     gene            complement(2303..2965)
                     /locus_tag="SCJ86_001088"
     CDS             complement(2303..2965)
                     /locus_tag="SCJ86_001088"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HEG1168666.1"
                     /translation="MKSSLQSQAYQAIRKQIIYADLEPGRKISEKALEEHLAIGRTPI
                     REALIQLRQQELVYTIPQSGTYVSKINLVSAENARFVREQLEEQIMIECCSKLDTQTR
                     IVLETILEEQEKAVKINDERAFFRADNLFHEACFEIAGRKEVWNWLDDHNTHLERFRW
                     LRVTIQGLPWETIMEQHYDLLKALVDKKPEEARFQTGLHLHMMLKEQETVTQRYPEYF
                     QQ"
     gene            3149..4795
                     /locus_tag="SCJ86_001089"
     CDS             3149..4795
                     /locus_tag="SCJ86_001089"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannitol dehydrogenase family protein"
                     /protein_id="HEG1168667.1"
                     /translation="MIDLNNWRNFIPELSQKEVEIPHFDRASLRENGQKNPVWIHFGG
                     GNLYRGFHAEIAQRLADLGELSTGVIVCETFDEEIIERAYQDFDDLILEVVMPSSGEF
                     VKRILAATADSLYCHPDCSEHFDKMIRHFENPSLQLVTFAITEKGYRLKDTNNKYIGP
                     IAVDIKNGPKKPSHTMSILAVLLYKRFLSGAWPIALVSTDNFSQNGSQLRTSLLTIAK
                     EWQKEGYVQNTFIQYLSNEEIVAFPWTMIDRITPNPSLSVSKMLAKEGFSNMSIFQTA
                     KGNNIAPFVNTEDIHYLIMEDHFPNGRPKFEKAGVFLTDRATVDKADTMKVTTCLNPL
                     HTSLAIMGCLLGYRSIAEEMKDPDLSLLVSEIGYNEGLPVVESPGIIDPKIFLEEVIT
                     VRFSNPNIPNMPQRIATDTSQKVAIRYGETIKKYMEIEVKDPQTLVFIPLTLAAWLRY
                     LIGIDDQGKPFIPSPDPLLTDLQHKLSDIQLGTQDTSHIHEKMAPILSNKEIFGVDLY
                     QAELGRKIEEMFIKMVAGPNAVRKTLQDYLQEHGGNLSWK"
     gene            4786..5868
                     /gene="uxuA"
                     /locus_tag="SCJ86_001090"
     CDS             4786..5868
                     /gene="uxuA"
                     /locus_tag="SCJ86_001090"
                     /EC_number="4.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333565.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannonate dehydratase"
                     /protein_id="HEG1168668.1"
                     /translation="MEMTFRWYGSQEEKITLDEIRQIPAVKGIVGTLMDIPVGEVWPR
                     ERIKELKEEIESRGLTLKVIESVNVHDAIKIGLPSREQYIENYKQTIRNLAEYGIEVI
                     CYNFMPVFDWVKSDLDYRLEDGSSTLAFISDDIPDDPREIVARIEHSSNEFGLPGWEP
                     ERLAPIKSLLEAYASVDEEKLRENFAYFLQSIIPTCEEVGVKMAVHPDDPPYSIFGLA
                     RIVKNREDLDWLCRIVDSPYNGITLCTGSIAEDPENDVYGILAEFCQRDRIPFAHVRN
                     IKFLANRDFYEAPHKSEYGSLDMYRILKTMYDNGFNGYIRPDHGRMIWNETGRPGYGL
                     YDRALGAVYLNGLWEAIEKGNKSKDN"
     gene            complement(5976..7529)
                     /locus_tag="SCJ86_001091"
                     /pseudo
     CDS             complement(5976..7529)
                     /locus_tag="SCJ86_001091"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738699.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
     gene            7677..8818
                     /locus_tag="SCJ86_001092"
                     /pseudo
     CDS             7677..8818
                     /locus_tag="SCJ86_001092"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318369.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="LPXTG cell wall anchor domain-containing
                     protein"
     gene            complement(8944..10023)
                     /locus_tag="SCJ86_001093"
     CDS             complement(8944..10023)
                     /locus_tag="SCJ86_001093"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter"
                     /protein_id="HEG1168669.1"
                     /translation="MNNVFIFCYLEVVDLTVYQRFLANEKIRRLTVLLFVVFVLFLVR
                     SMITTILLTFIFTYLMIHLVQIIQRFVRIPTGVLAIMIYTLIVYLIYLAFTKYIPMLI
                     HQTFDMIQSVINFYEHQPKDADPVLQYIHTYIERNDFFEQLQNGASIALGYLQDIGKM
                     AVAFAMSFILSFFFMIEKKKTIVFSRLFLKSEFSWFFQDIYYFADKFVNTFGLVLEAQ
                     FVVALLNTLLTTIALAVFGFHQLLSLAIMIFILSLIPVAGVIISCIPLSFIAYSQGGI
                     RDVVYILITILIVHLIESYVLNPKLMSSKTELPIFYTFVVLLVSERFFGVWGLIVGIP
                     IFTFFLDVLKVKKIPNHLPKETDIP"
     gene            complement(10126..10566)
                     /locus_tag="SCJ86_001094"
     CDS             complement(10126..10566)
                     /locus_tag="SCJ86_001094"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335722.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HEG1168670.1"
                     /translation="MEDLYLVNQVCFPLYAASKEIIRHYTPLLKPLNLTYTQYLVMLV
                     LWEKKKTSVKELGNDLCLDSGTLTPLLKKLAEKNYINRQRETGDERIVNVTLTAQGAA
                     LQKQAEGIPQKIKSYLPLEQEELDTLQHLTRKMLDNFHQDQKKG"
     gene            complement(10623..11033)
                     /locus_tag="SCJ86_001095"
     CDS             complement(10623..11033)
                     /locus_tag="SCJ86_001095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288163.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="organic hydroperoxide resistance protein"
                     /protein_id="HEG1168671.1"
                     /translation="MKKMYSTKMINTGGRSGEVHNPDRTFELNIAAPGKRVENATNPE
                     QLFAAGFSACFNSALELIKQQKNISGASTVSAQVSLYSESETSFVLGVEIEGSIEGLS
                     LEETQELLEAAHKVCPYSKATAGNIEVTLKAVSE"
     gene            complement(11213..11647)
                     /locus_tag="SCJ86_001096"
     CDS             complement(11213..11647)
                     /locus_tag="SCJ86_001096"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288162.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HEG1168672.1"
                     /translation="MHKGTEIDFVLNDSLKALELYQTIFDEIQLIEATDYPRGNNEVI
                     FTLYGTRFHMLDENTSLGLTAPTAEHPNTIWFNIIVSDIEDTHNKALRNGCVEIQEMT
                     EMMDFGVTNSMFRDPFGYLWMLHQVHREVIFEERKQLFEKYM"
     gene            complement(11747..12595)
                     /locus_tag="SCJ86_001097"
     CDS             complement(11747..12595)
                     /locus_tag="SCJ86_001097"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314986.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YitT family protein"
                     /protein_id="HEG1168673.1"
                     /translation="MKETRQFIWTYIKIIFALFILGVAINMFLGPHHIAAGGVSGLGI
                     LLEAALGFDRAIVILVLNIVMLVLALLFLGKKPFFKVLFGSLVFPLIIAIVPETMITS
                     DRLLSVIFGSAIFALGVAILYKNNSSSGGTTIPPLIFKKYFHLNTSIGLFFTDAFVVS
                     LNLFIFGIEEFLYAILSIAITSIVMTYIETGINRKKSVMVMSEHYLEDIRLRLSAEIG
                     RGLTLLEAKGGYNRKSKEVLLIVVTDHEFSRMKPLIEEIDPSAFVIVSSVAEVMGRGF
                     TYHPIE"
     gene            12984..14750
                     /locus_tag="SCJ86_001098"
     CDS             12984..14750
                     /locus_tag="SCJ86_001098"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008271692.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-glycosidase"
                     /protein_id="HEG1168674.1"
                     /translation="MDTAAIYHRPESEYAFLYTNTRFRIRLRTRKNDIKKVYVLCGDP
                     YTITTEKWYQKQRPMKKCLSTNVHDYWEIEVTSETSRLQYAFHVVGEDNTDCFYGDQG
                     IFPYRENVITEPNFYFRIPYFHQIDRFTIPEWVKETVWYQIFPERFANGDKANDPEHT
                     LPWGSKDPDREDFFGGDLQGVMDHLDYLTDLGINGIYFCPIFKATSNHKYDTIDYYEV
                     DPAFGDKKLLKNLIDEAHKRGIRIMFDAVFNHMGVHSPQWQDVLKNGEKSIYKDWFHI
                     RFFPVDSYQMTDLPETAENIPYDTFAFTPFMPKLNTANPEVQKYLLDIATYWIKEFDI
                     DGWRLDVANEVDHHFWKKFREAVTEIKPDIYILGEIWHSSQAWLQGDEFHAVMNYAFT
                     DSIKDYFAKKKITASQMVSGMNHQQMLYRDQVNEGTFNLLDSHDTARILTLCQGNKEL
                     MKSVMAFMFLQKGSPCIYYGTEIGMTGEDDPDCRKCMIWEKEEQDLELFGFIKELVSL
                     RKQLSKIISEGSTQWLIVNDREDKLYFTRKLEGQIIYVYFNQSKEPWVVEQENEVILS
                     QNCQLLEDGKAEIRQYGFLIMC"
     gene            14852..16111
                     /locus_tag="SCJ86_001099"
     CDS             14852..16111
                     /locus_tag="SCJ86_001099"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346576.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HEG1168675.1"
                     /translation="MKKRSVKQLGLGLISLGILGGLAACGNGNSSAEGEADEGKTLTV
                     SVDSGYKDYINEIKNTFEKENDVKIKLVEKDMFDQLESLALDGPAGKGPDVMMAAYDR
                     IGALGQQGHLAEVKLGNEAAYDETDKAQVTYDGKIYGEPAVIETLVLYYNKDLVDTAP
                     STFKDLENLSKDSRFAFESEAGKNTGFLAKWTDFYYSYGLIAGYGGYVFGDDGTNPSD
                     IGLNNAGAVEGISYATDWFKNVWPKGMQDIKSAGDFASQQFMSNKTAAIIDGPWQAQT
                     YKENNINYGVAKIPTLNNGQPYQPFGGGKGWVVSNYAKNKDLSQKWLDYVTNQENQEK
                     FFEMVNEIPANQQARETAKAKNDELTTAVIEQYETAQAMPNIPEMGEVWTGAENLMFD
                     AASGSKTPKESADEAVKTISEAIEQKY"
     gene            16258..17559
                     /locus_tag="SCJ86_001100"
     CDS             16258..17559
                     /locus_tag="SCJ86_001100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400236.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HEG1168676.1"
                     /translation="MQQDKTSNINEKKITLCSLIPGMGQFVNGQAFKGIVFFAIFVLF
                     IGQMLLGGFHALVGLVTLGSIPMEDHSLFILIEGTLQFIITIIFLIFYGLNLLDAYRV
                     AKMRQEGKKINRTMKEVIHNIGDEGFPYLLTLPAYLLMIFTIIFPVLVTLFTAFTNYD
                     FNHIPPASLIDWVGAKNFFSIFFLSSYRNTFFAVFSWTVIWTLCATTLQITLGILTAV
                     VAHQKFIKFKRIFGIIFLLPWAVPAFITIMSFSNIFNDSVGAINTQVIPFINQLPFVD
                     ISAISWKTDPNWTKVAIILVQGWLGFPYIYVMVTGILQSISEDLYEAAKIDGANAWQR
                     FSNITLPAVFLVAAPTFITQYTGNFNNFSMIYLFNNGGPGSVGGGAGSTDILISWIYK
                     LTTGTSPQYSMASAITLIISVIVICVSLLVFKKTNAFNMGD"
     gene            17562..18413
                     /locus_tag="SCJ86_001101"
     CDS             17562..18413
                     /locus_tag="SCJ86_001101"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010750943.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HEG1168677.1"
                     /translation="MKKFHSSARFQKRLSHFFTYFFLLILSVIIIYPLLITASTAFKS
                     GNIAAFSLDLSSDWTLGNFTRLFSETLYGTWYKNTLIISVLTMIVQVTVVTLAGYTYS
                     RYRFIGRKSSLMFFLVIQMVPTMAALTAFYVMAFLLEALDKYWFLTMIYIGGGIPMNT
                     WLMKGYFDTVPIDLDESAKLDGAGHFRIFFQIVLPLVKPMLAVQALWAFMGPFGDFML
                     AKFLLRTPENQTVAVGLQTFISDVTNQRVSLFAAGAILIALPICLLFFFLQKNFVTGL
                     TSGGTKG"
     gene            18426..19241
                     /locus_tag="SCJ86_001102"
     CDS             18426..19241
                     /locus_tag="SCJ86_001102"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376626.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="maltodextrose utilization protein MalA"
                     /protein_id="HEG1168678.1"
                     /translation="MQTFPLNYFSALRTPTQVFAGRKLLSWPKFFLIFVFLVSLMVMP
                     VTLFYANQIQAIPLEQFLSVHSLIDEQGTQKFSELELSETGLQASQQTIAVTPEILVG
                     VSLSEKQQSDHGTFIDFEKEQWVIQQKDKSGIRRYTMNYSPSFQPDSVRTPEDFQRFL
                     EREFYASNRPTIILSYSLSLGLVLFVMTSLILFGASFFLWMTRKSQLSSIHTFKESAN
                     LMLNVMGIGSMAAAVVGFIHFDFILMLSVQTTITVLLLLWVFVKTRFKDKRVE"
     gene            19392..20558
                     /locus_tag="SCJ86_001103"
     CDS             19392..20558
                     /locus_tag="SCJ86_001103"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002369828.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HEG1168679.1"
                     /translation="MLEKTLYNQLFSRSFSLPVEVNYWDGTTKQYGDTDTPPQIKINI
                     HEEIPMKEITSNASLALGEAYMDHRIEIEGSIQALINDAYQQANSFLRGKDYLKWLPK
                     KEKHTKQKNKEDIHAHYDLGNDFYKLWLDPTLTYSCAYFNTPEDTLEQAQINKVHHIL
                     DKLFIKEGETLLDIGCGWGTLMFTATKEYNVKATGITLSEEQYDLITKRIKEEHLEDK
                     CRVLLMDYRELKGETFDHITSVGMFEHVGSENLEGYFKVVKDLLKPKGTALIHGISRQ
                     QGGATNAWINKYIFPGGYVPGVTELVDNITKNDLQLIDLESLRRDYQLTLEHWTKNFH
                     QVQDQVTKEMGDSFYRMWDLYLQACAASFESSNIDVIQYLLVQPGNNDIPMHRA"
     gene            20788..21435
                     /locus_tag="SCJ86_001104"
     CDS             20788..21435
                     /locus_tag="SCJ86_001104"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738701.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PepSY domain-containing protein"
                     /protein_id="HEG1168680.1"
                     /translation="MKKTVIYTSLFAALFLAACQSNTNQGTSGNTSSSSEQRSSQVSD
                     SSTKASTSSSSQTKSSSEASTQSDIKVSAARAIELFQDKYPDTAITSLELDSDWGSYF
                     YKIEGVDDQKEYEARIDAQNEKMETENPETLDRDEQNGQKKKEDGLDVDKLITIEEAA
                     KIAVDKVGSGTATDWDLDKDLGTTYWEVKVKDGNQSTSVKINSQTGEVLSTEQDD"
     gene            21519..22463
                     /locus_tag="SCJ86_001105"
     CDS             21519..22463
                     /locus_tag="SCJ86_001105"
                     /EC_number="1.3.98.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290739.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroorotate oxidase"
                     /protein_id="HEG1168681.1"
                     /translation="MSLETTFANHIFANPLMNASGVHCMTTQELDELAHSEAGAFITK
                     SCTINERKGNPEPRYFDVPLGSINSMGLPNLGFSYYLEYALAYEKVQENQNQPLFFSI
                     AGMSVQENLEMLEKIEKSGFNGITELNLSCPNVPGKPQLAYDFEATYETLKEVFSIFS
                     KPLGIKLPPYFDFAHFDQMADILNQFPLTYVNAINSVGNGLYIDTEQEAVVIKPKEGF
                     GGIGGEYIKPTALANVRAFYTRLKPEIQIIGTGGIRTGQDAFEHLLCGASMLQIGTEL
                     HKEGPEIFSRIIKELTQIMSEKGYTSIDEFKGKLRTIS"
     gene            22598..24049
                     /locus_tag="SCJ86_001106"
     CDS             22598..24049
                     /locus_tag="SCJ86_001106"
                     /EC_number="3.5.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332411.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidase"
                     /protein_id="HEG1168682.1"
                     /translation="MNDATELAKELKEKEFSFQELVTDIQKKIKKQNHELNAFVALEP
                     FESNSHELLIPQSEKGPLAGIPFPLKMLGQEKKGWLATSGSRLFETHRASHTSNYVRQ
                     AEAIGLVPFGQTNAPEFGFKNITDPVIYGPARNPWNLECTPGGSSGGAAAAVSSGIVP
                     LAGASDGGGSIRIPASFCGLIGLKPSRGTMPVGPYAWRGWQGAAIDFGLTVSMRDTEA
                     LFYGMRSIHSGAPYQVSPVEWQTHPRKKRLKIAFCVDSPIHSQISEEAVLAVKKAVDF
                     LGKEGHDLTEIPYPLDGRKLITSYYQMNGAETAAMIRSIETGIQRAVRKEELEPISWT
                     LFQYGQKLSAATYIDSLHVWDEAAIVMENLHQEYDLFLSPTTAFTAPKISEDLQSDAI
                     RAQMEHISDCSEVEGLDVVSSMFEESLKITPYTQLANLTGQPAISLPTHLTKENMPLG
                     IQFMAARGREDLLFQIGYLFEANHQFHLPNPWK"
     gene            24070..24906
                     /locus_tag="SCJ86_001107"
     CDS             24070..24906
                     /locus_tag="SCJ86_001107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343290.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transporter substrate-binding domain-containing
                     protein"
                     /protein_id="HEG1168683.1"
                     /translation="MKKKRLFALITSAFFILSACGTAAANQEDNSWSNVEKKKELVVA
                     TSGTLFPASYYNDQNELVGYDVDLAKEVAKRLNVKISFKEYNVDGQITSVSKRESDFA
                     ANDFGITKEREKKFSLSETFKYSFDSMIVRKKDDSGIHSLEDLKGKKAAGEPNTSYMR
                     LAEKHGAKLVTYDNATNDQYLSDVANGRTDVILNDYYLQKMAVSALPDIPVKILENVY
                     FNENESGFLLKEGNDALKKKIDGVLKEMKEDGTTKKISEKYFQTDVSVKPKETMAESV
                     SE"
     gene            24906..25565
                     /locus_tag="SCJ86_001108"
     CDS             24906..25565
                     /locus_tag="SCJ86_001108"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008265363.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter permease"
                     /protein_id="HEG1168684.1"
                     /translation="MYFPLIDFSLMWTSLPFVLQGLVYTLGIGFVSFLLGNLVGLLLT
                     VLGLLDWLPLNVFIRFYLSFFRGIPALVLLFLLYFGLPYQLSALTASVICFTITSSAF
                     IGEIYRGSLAGVSSGQWDAGLASGFSFFGVLKYIVLPQAFRISVPALSNVAMDLVKGT
                     SLAATITVPEIFQKAKIIGGRTFDYFSLYILVALIYWVICIGIEKVQQYLEKRSIHQS
                     I"
     gene            25691..26380
                     /locus_tag="SCJ86_001109"
     CDS             25691..26380
                     /locus_tag="SCJ86_001109"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853260.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEG1168685.1"
                     /translation="MKTPLTIEIESSLYQYCIEQGGLVVEEVTMPDDQGIVDTLSCLT
                     KFDGSREWRCYELKVTKADFRSTAKLSFVGHYNYFVLPSKLYEEVKTEIPTGIGVLVY
                     RPYTVEEEMPASGTFLIAKKAAKKELAVPEEALTNRFMASLFREVRKAKQMERGVKYF
                     PKDQLYKELKRRSENQDPFSNERYYERFLEEIASQAINELQEEVIALQHDYEYLRQQQ
                     QIRRLPTEPLE"
     gene            26386..27342
                     /locus_tag="SCJ86_001110"
     CDS             26386..27342
                     /locus_tag="SCJ86_001110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302005.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sce7725 family protein"
                     /protein_id="HEG1168686.1"
                     /translation="MYYPYLRGKQFDLLALKEALSRGLLSNKIQPVIEPVRDSATLKN
                     VIELFQKKHHPIAVIQNPQVGQFKLFDQHVHSWTVKENSSVVPAQILTPENLSEVLDS
                     PPAFLVFDGQHYPKDTEVWKQLAGVDSKFLIPDTSRFRIWLPENKIVIRDSFQTRKHV
                     ESYADKTDDFFSDDYLFFHADGYIGFSDFTIEGSRYFDKGFPSRALAIHLTYIDAYGN
                     IRVKHFVSDSNDSAKDQALKFLEAGDKMKKWIMRHHHQLLITSGMIELLALYQQQKFP
                     GLGVLKKWSLMHHLELISHLLDHPKDWLKYYSKVQELYEVIQ"
     gene            27386..27898
                     /locus_tag="SCJ86_001111"
     CDS             27386..27898
                     /locus_tag="SCJ86_001111"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302625.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEG1168687.1"
                     /translation="MYIRQTRKEEVEEVVEIINAAADFIASQGSPQWQNGQKPTKETI
                     LNDIERKESYVLIVEDQIAGTAALVSGVDPVYTKIDGSWKESTGPYLSIHRVALAEST
                     RGKKLAKPFLEHLLTIAASKNIQDIRIDTYPANEPMEKTIYSAGFTYQGMIEFPFEHG
                     ERKAYQIIIN"
     gene            complement(28154..28702)
                     /locus_tag="SCJ86_001112"
     CDS             complement(28154..28702)
                     /locus_tag="SCJ86_001112"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295911.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HEG1168688.1"
                     /translation="MNYCYVFIGPSGSGKTSLAEAIFQPEQKIITYTNRSPRPGEKDQ
                     EDYYFVSDNTFNQMIKGNEFAEWDQYAQHKYGSSKAEIKKKLTVGNCYAVLTANGFWH
                     LYEYFGTVVRPIFITISKETLQSRLKKRGDSSEQISARLAVYVEDLAQLKRLKAIPSL
                     LVVSNDGAFQEAADKIKKGLLI"
     gene            complement(28791..29972)
                     /locus_tag="SCJ86_001113"
     CDS             complement(28791..29972)
                     /locus_tag="SCJ86_001113"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NupC/NupG family nucleoside CNT transporter"
                     /protein_id="HEG1168689.1"
                     /translation="MSYLLGIIGLFVTLGLAFLISSNKKAIKLKPLIVMFVLQLVLGF
                     VLLRTTFGTAVVSVLAKMFDHLLAFAGQGVDFVFAGVANKGSAPFFLSVLMPIVFISA
                     IIGILRYIKILPLFMKAVGLGLSKINGMGKLESYNGVASAILGQSEVFISIKKELPFL
                     SEKRLFTMSVSAMSTVSMSIVGSYMALIDSKYVITALVLNLFGGYILASIVNPYVLEE
                     KEDELIIEENKEQTFFQMLGEYILDGFHVAITVAAMLIGFVALIAMINAIFHGIFGIT
                     FQEILGYIFAPLAFISGIPWKEAVDAGSIMATKLVTNEFVAMTELANGNFHFTERTTA
                     IISVFLVSFANFSSIGIISGAMKGLNEEKGNLVAKHGLKILLTATLVSFLSAIVTGLL
                     V"
     gene            complement(30208..31716)
                     /locus_tag="SCJ86_001114"
     CDS             complement(30208..31716)
                     /locus_tag="SCJ86_001114"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1846 domain-containing protein"
                     /protein_id="HEG1168690.1"
                     /translation="MKKIGFDSEKYIEEQSAYILERVNHYDKLYLEFGGKLVDDKHAK
                     RVLPGFEEDAKIKLLQKLKDQAEILICVYAGDIERNKIRGDYGITYDMDILRLIDELR
                     GYGLLINSVVITRYSGQPATKVFINKLERRNIKVYKHSEIEDYPVNVEKIVSEDGFGK
                     NEYIETTKPIVVVTAPGPGSGKLATCLNQLYHESQKGHAAGYSKFETFPVWNVPLKHP
                     LNIAYEAATVDLKDVNMIDSFHFDAYNKVAVNYNRDVETFPVIKRIIEKITGKESVYQ
                     SPTDMGVNRVGFGIVDDTVVQEASKQEIIRRYFQTECDFKKGLIDEEAVNRIKLIMEE
                     VGLRPEDRNVVVPAHDYADKIQAQSPTKDPSAVIAIELPDEVIITGRTSSLMDASAAV
                     ILNAVKHLAHIADDIPLLSPLVLETIQGLKSKTLHSSLDALSINEVLIALSISAVTNP
                     IAQVAYDKLAELEGAQAHSTVMVNKNDEQTLKKLGIDITCAPVYPSENLYYQ"
     gene            31875..32066
                     /locus_tag="SCJ86_001115"
     CDS             31875..32066
                     /locus_tag="SCJ86_001115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEG1168691.1"
                     /translation="MKKRQKKKNAYKHYIRSIFTGYERMLEDPELEQLTFTYLNEETQ
                     LTRDEHQRIHFTTRDLPSK"
     gene            32269..33186
                     /locus_tag="SCJ86_001116"
     CDS             32269..33186
                     /locus_tag="SCJ86_001116"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376643.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="viral A-type inclusion protein"
                     /protein_id="HEG1168692.1"
                     /translation="MKEPDTNANYEPDELEETNADQDTGQESSSFYDIKEKASMNLSQ
                     LLEDLQAFEKAVREEDIPEIYRLYNGRLNEELKRSSNENHEIDQLLMRKIHDRFLQEF
                     PFMEQVENISPTMSYYKIGTYYHDRPTVGIDASLPEIFVLPKIDEEWEKYSQPESTDY
                     DKKINELDAKVITAQTEIERLDEQIKEINRQMTDLNDNKGFLNRKKIEEEIQELEKKK
                     QVLSNEKLGWLPYIETPETIQQQKEALKQEARADQLRAAIVEKEQRQIRRYFGSKEGF
                     GQAIHEFLMNYLGNENPNNQSNEGGSEYE"
     gene            33179..34252
                     /locus_tag="SCJ86_001117"
     CDS             33179..34252
                     /locus_tag="SCJ86_001117"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEG1168693.1"
                     /translation="MNEHDEAEWTKNEEQTEDHEETNTSLEEQTSNPLDDTEEIPMLE
                     ETKIHEKEKVTPTNTNTAENTHAYEITENPGYLEGKEQDHQDIVSLIETAEAELADLR
                     MRKDLIESDFPQLLESYDRFILNEEPISTIGKTSLIEYFTYELLKPLNDIGLERSNSA
                     YDTWKTRHFSINYTLNGQNELVFSFVLPTIDQRYQVESMSLLNVSPETMEINVQDDRV
                     LSLIRHWSVDRVFSAGQITIFNHKLNQLLAHARTLGFTVNQTLLDNTKPLRLNLQSEF
                     ELTNEVLDDIFIVAMKNPSYDMEKINETTYQVRLDKGQSLTITTNEKDQTVLSISSGD
                     YPRSVIDFFINYEFLVPLMVRKS"
     gene            34400..34918
                     /locus_tag="SCJ86_001118"
     CDS             34400..34918
                     /locus_tag="SCJ86_001118"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338281.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEG1168694.1"
                     /translation="MSTNKIAQLSLLSAACVAGRIAFQFIPNFQPMTAIFLFIVLYLS
                     LKDALIVMSLSIAISSFYFGVGPWVIGQWISYTVVLSLFSIVCLRKTVQRNLWLKGVC
                     FFLAGMVYGIGMTVFDTLLYRLPQPWIYYLQGVSFDLMHSIGNVVFFLVFLPVVKRFY
                     NHSGGKNEKNNL"
     gene            34899..35291
                     /locus_tag="SCJ86_001119"
     CDS             34899..35291
                     /locus_tag="SCJ86_001119"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4430 domain-containing protein"
                     /protein_id="HEG1168695.1"
                     /translation="MKKIIYSLLAMSVSILILTSCSTTYTQSASNQKEEVKTSQKATI
                     TLQENGQNFSEKEVVFKKGDDLLSILKRNFEVKEDNGFITSLEGHSQDEKNQLYWMFT
                     VDGKSATTGAKEIKLNDGQSVIFNLSKL"
     gene            35367..36302
                     /locus_tag="SCJ86_001120"
     CDS             35367..36302
                     /locus_tag="SCJ86_001120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631178.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation diffusion facilitator family transporter"
                     /protein_id="HEG1168696.1"
                     /translation="MENFKKSGMFSVIAALGANILVAISKFVGYAISGSAAMLNESIH
                     SIVDCSNQILLLFGDKQANKGQSELHQFGEGRAKYFFSTIVAMMLFFGGGALGVMEAF
                     EKLTHPSHEVGNPIIVIAILLFGLVIEGSSLRVAMKEIKELNTEKLSTMRFLRESRHS
                     EILIIFTEDLCAVIGLVLALAGTILTYATGIAAFDAISGLLIGFLLMGAAIFLAKEFY
                     SLIIGESVTASDLSKIKLAFERPEISRLIDIKTVHLSPTEILVAAKADIVGNKENEAY
                     SVINDIEKNMRQSLPDKKMYIYIETDKYDPNYSRK"
     gene            36435..37214
                     /locus_tag="SCJ86_001121"
     CDS             36435..37214
                     /locus_tag="SCJ86_001121"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315001.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AzlC family ABC transporter permease"
                     /protein_id="HEG1168697.1"
                     /translation="MNKKKSAPWLQALHAVMPLCISYIPVGLACGVLLQKVGFDPLLS
                     GLLSILVFSGGAQFLVASMLTTQASFATTLLMVFFLELRYTLLGASLAGFMKKEKRTF
                     LAVFSQSLNDENYAVNYLKFSTDPSWNKRKALYVNWFSMTAWAGSNMIGNFFGSVIRV
                     DADLVHFALTAMFIFMFIMQMKSALLIFTGLFSGVLGVVFMVLFQNTFGLIAATVIAS
                     FAGFMLEKAFKKEKAKKLRDRGKDVNEPLFNFPEQEANHHD"
     gene            37207..37524
                     /locus_tag="SCJ86_001122"
     CDS             37207..37524
                     /locus_tag="SCJ86_001122"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005877655.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AzlD domain-containing protein"
                     /protein_id="HEG1168698.1"
                     /translation="MTKLYLLLLVACLFIVAYIPRLFPMLYFTHRKVPSWFSDWMKYV
                     PVALFAALAFKDVFITHEHLDIAWNIKIAAMILVAGVAYKTRSMALSVLTGLASVFLL
                     SML"
     gene            37768..39378
                     /locus_tag="SCJ86_001123"
     CDS             37768..39378
                     /locus_tag="SCJ86_001123"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide biosynthesis protein"
                     /protein_id="HEG1168699.1"
                     /translation="MNKKLMQGTFWLTFANLLCKVLGVVYLIPWLSMMGNNQDGMLAT
                     TLYNVGYLPYGLFLMLGTVGFPNAIAKKVAVATKEGDNAACRLIFRSTINIMFVIGIA
                     SAALMYLFAPLLAGISPIANVNNGILAIRSLCPSLIAIPILSAMRGYFQGKNDLRPYG
                     TSLIIEQAVRVIVILAGTYYLRVLTDGTILEAVLISTVASFFGGLAAIIHMYIVGRRK
                     DFFELKDFLISSRYFERENRSASVSIIRETLPFIFVGSVITIVQMIDQVTMKPLLHFF
                     RPEIADQQLEFLFSRASVNPNKLTLILISMVGTVAISSLPILSTMKKKDRIQIEKTVG
                     DSFSIALLILLPSLTGMSLLAGPLYTLFFGYDPESVGYFQMALLASLFFSLFTILSTM
                     LQSLNHHRFAIRLTVEAIILKVIFQVIGLGLVGGYGMSLSNGLAFGIVFVRGYHFMCK
                     EYRIAPLAKISNFFLKTFRSTLIMLAVCFAVFFLLNQRLSMESKSYAMIYCVAVGSIG
                     GLVFLFSQFGKNGFRMLKNVKHHSRSKE"
     gene            39535..40383
                     /locus_tag="SCJ86_001124"
     CDS             39535..40383
                     /locus_tag="SCJ86_001124"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340641.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DegV family protein"
                     /protein_id="HEG1168700.1"
                     /translation="MKLAVVTDSSAYLPERIKNHPDLFVIPIPLIMDGKIYNEGIDIE
                     ADEYYGLLKNSKDFPTTSQPVVGEVLGLYEELKEKGYDTILSIHLSSGISGFVNTLFA
                     MKDDISGVTVIPYDSKITSMPMGHMVEAALDLNDEGQSLDEILAHIDRIRDNTYAYLI
                     VDDLNNLVRGGRLTNGAALIGSLLKIKPILTFSEGKIVLFEKIRSSKKAFARAEEIIG
                     RRNNEIQPPVKLYVIHANNLEVAIAEKEKLQKKYPNAEIEIGHFGPVIGTHLGEKAIG
                     IAISAQ"
BASE COUNT        13231 a         7260 c         7799 g        12124 t
ORIGIN      
        1 aaaattcttg tcaccatcct tttttccgct ctctgacttt ttacaatctt catagctcaa
       61 gtgcgcatca gataatcgaa tgctcctaaa gctgcagtag ctcctgaacc cattgaaaca
      121 atgatttgtt tgtatggact atctgtacaa tcaccagctg cgaaaacgcc ggggatattc
      181 gttgcgccat ttttctcggt cactatttca tttcttggat tcatttcgat cgttccatct
      241 agccaatccg tattagggag taagccgact agaatgaata ctcctgaaac agctagtgta
      301 tgcatctcca tcgtacggcg atcgatatag gagagagatt ctacctgatt cgtaccgctg
      361 atttctttcg tcgctgcatg tttgataaca gtgacattcg caagagagac tagtttatcc
      421 tgtaagattt gatctgcttt taattctggt aagaattcta gaacatatac atgttttgct
      481 aacccagcca aatcgattgc tgcttcaaca ccagaattac caccaccgat aacagcaatc
      541 tcttttcctt taaataaagg gccgtcacag tgtgggcaat aagcaattcc tttattttta
      601 aactcttttt cgcctggtac attgatagaa cgccagtgcg cacctgtcgc gatgatggcg
      661 gtttttgctt taagtgatat accgttagct aagccaatat taataaaatg ttgctcttta
      721 tttaaggaaa cggcctgttg attcatcata atgtcgatag ggtaactacg cacatgactt
      781 tctacttgct gcatcaattt tggaccttct gtgtaagagg taccaatcat attttcaatc
      841 cctagtgttt caacgacttg tccgccgaaa gaatcagcga caagtcctgt acggattcct
      901 ttacgtgctg catagatagc tgcactacta gctgctggtc caccgccaat cactaataca
      961 tcataaggtt ctttttcaaa caactcttct tcaatcaaag ggccagtgat tttttcaaca
     1021 atttgttcga tcgtcatgcg tccgctacta aattcttctc catttaaaaa cacagttggc
     1081 actgccatga tttttttatc atcgatttct ttttggaaca tcccaccttc aatcatcgta
     1141 tgagtaatat ttggatttaa tacagctaat atattcaatg cctgtacaac gtcggggcaa
     1201 ttatgacaag tcaagctaac ataactgtca aaatgcaatg tttgtgttat ttttttgatt
     1261 ttaagactca aagattcact gattttcggt gcccgtccac taatttgtaa catagccagt
     1321 aaaaaagagg aaaattcatg accgaggggg ataccagcaa aaacgattcc actttctttg
     1381 ccaactggat tgatagaaaa actaggtgtg cgcatcgaat ctcttttttc ccaagagatt
     1441 ttaggagaca tttctgctat ttccttcgta aattcagcta gtttttgact attttcacta
     1501 ttatccaaac taacagaaaa aacaatagga gattcaatca gtgctaaata ttgttcgaga
     1561 ttttgtttag tttctttgtc taacatatcc accacctaaa tctttccaac aagatcaaaa
     1621 cttggtttca atgtttcgcc actttctttc cattttgccg gacaaacttc acctgggtgg
     1681 gtacgtacat attgagcagc gcgaatctta tcaatcagtg cactagcgtc acgaccgatt
     1741 ccatctgcat tgatttccat agcttgaacg attccgtctg gatcaataat aaatgttcct
     1801 cgctgagcta atccttgctc ttcatccaaa acatcaaaaa tacgagaaat ctgatgagac
     1861 ggatcaccga tcatcacgta ttcgatttta ccgatagcat ctgatgtatc atgccatgct
     1921 ttgtgagtaa agtgggtatc ggtagagcaa gaaaagactt caacacctaa agatttcaat
     1981 gtactgtatt gttcttgtaa gtcttctaat tcagtaggac aaacaaaagt aaaatcagct
     2041 ggatagaagc aaagaatact ccagttcccc ataagatctt tttcactgac ttctaaaaat
     2101 tctccttgat gataagcatg tgcattaaat tcttcgattt ttttcccaat caatgacata
     2161 gtaaaacctc caataataat tatttattta taattattgt aatttagttg gagttgcaaa
     2221 gtcaatggat attttgttat ttaataaaga ttactcaaaa acgaaggtta attataaaaa
     2281 tagaagaggt cgcaagaaaa tgctactgct ggaaatactc gggataacgt tgagtaacag
     2341 tttcctgttc tttcagcatc atgtgtaagt gcaatcccgt ttgaaatcga gcttcttctg
     2401 gttttttatc aactaaagcc ttgagtaaat cataatgttg ttccatgatc gtttcccatg
     2461 gaagcccctg aatggtcact cgtaaccagc ggaaacgttc taaatgggta ttatggtcat
     2521 ctaaccagtt ccaaacttct tttcttcctg caatttcgaa acaagcttca tgaaataaat
     2581 tatctgcacg gaaaaaagcg cgttcgtcat ttattttgac tgctttttct tgttcttcta
     2641 aaatcgtttc tagcactatt cgtgtttgtg tgtccaattt ggaacaacat tcaatcatga
     2701 tttgctcttc caactgttct cggacaaagc gagcattttc tgcagaaaca agattaattt
     2761 tggaaacata cgtaccactt tgaggtatcg tatacacgag ttcttgctga cgtagttgaa
     2821 tcaatgcttc gcgaatggga gtccggccga tagcaagatg ttcctccagt gccttttcag
     2881 atattttcct tccaggttct aagtcagcat agataatttg tttacgaatc gcttgataag
     2941 cttgtgattg caagctgctt ttcatcttta ttcctccaaa atcatttcta tgaacgtagt
     3001 ataacagaag aggtctgaaa aacaatctgg tatgtcacat taactagtaa atttgtataa
     3061 atagtaaaat tttggttgac atagcgattt ttattgcata aaattcactt gaatacaaaa
     3121 actaatatgt tagtttagga ggggaaaaat gattgattta aacaactgga ggaactttat
     3181 ccctgaatta tctcaaaaag aagtagaaat acctcatttt gaccgtgcat cattaagaga
     3241 aaatggacaa aaaaatcctg tctggatcca tttcggtggc ggaaatcttt atcgaggatt
     3301 tcatgcagaa atcgctcaac gtttagcgga tctaggcgaa ttgtccactg gtgtcattgt
     3361 ttgtgaaaca ttcgatgaag aaataattga aagggcttac caagattttg atgatttgat
     3421 tcttgaagtg gtcatgccaa gcagtggaga gttcgtcaaa cgtatattag ctgcgacagc
     3481 tgacagtctg tattgtcatc cagattgttc tgaacacttt gataagatga ttcgacactt
     3541 tgaaaatcct agtttacaat tagttacttt tgctattact gaaaaaggtt atcgattgaa
     3601 agatacaaat aataaatata taggaccgat tgcagtcgat ataaaaaatg gtccaaagaa
     3661 gccatcacat acaatgagta ttcttgctgt gctactttat aaacgttttt tatctggagc
     3721 ttggccgatt gcacttgtct ctacagataa tttttcacaa aacggtagtc aattacgtac
     3781 ttctttgcta acgatcgcaa aagaatggca aaaggaaggt tatgtacaaa atacatttat
     3841 ccaatactta tcgaatgaag aaattgttgc gtttccatgg acgatgattg atcgtatcac
     3901 cccgaatcct tccttgtcag ttagcaaaat gttggcaaaa gagggctttt caaatatgtc
     3961 gattttccaa accgcaaaag gaaataatat tgctccattt gtcaatacag aggacatcca
     4021 ttatttaatc atggaagatc attttcccaa tggacgtccg aaattcgaaa aagctggggt
     4081 attcttgact gatcgtgcaa cggtagataa agcagatacg atgaaggtga cgacttgcct
     4141 taatcctttg catacgtctt tagctattat gggttgttta ctggggtata ggagcatagc
     4201 tgaggagatg aaagatccag atttatcgct gctagtttct gaaatcgggt acaatgaagg
     4261 cctcccagtt gtggagtctc caggtatcat tgatccaaag atctttctgg aagaagtaat
     4321 tactgtgcgc ttttctaatc caaatattcc taatatgcct cagcgaatcg ccacggatac
     4381 ctctcaaaag gtggcgattc gttatggtga gacgattaaa aaatatatgg aaatagaagt
     4441 taaagacccg cagacacttg tattcatacc attgactctc gctgcttggc ttcgatacct
     4501 tattggcatt gatgatcaag gaaaaccatt catcccgagt ccagacccac tattgacaga
     4561 ccttcagcat aagctttccg atattcaact gggtacgcaa gatacatcac atattcatga
     4621 gaaaatggct cctattttgt caaataaaga aatttttggt gtagatcttt accaagcaga
     4681 gcttggcaga aaaatagaag aaatgttcat caagatggta gcaggaccaa atgccgtacg
     4741 aaaaacatta caggattact tacaagaaca tggaggaaat ttatcatgga aatgactttt
     4801 agatggtacg gatcacagga agaaaagatt accttagatg aaatcagaca gattcctgcg
     4861 gtcaaaggga ttgtaggcac attgatggat ataccagtag gagaggtatg gcctagggag
     4921 aggatcaaag aactaaaaga agaaattgag tctcgtgggt taacactaaa agtaattgaa
     4981 agcgtcaatg tgcatgatgc cattaaaatt ggattacctt cccgtgagca gtatatcgaa
     5041 aactataagc agacaatccg aaacttggca gaatatggaa tagaagtcat ctgctataat
     5101 tttatgcctg tatttgactg ggtgaaatct gaccttgact accgcttaga agacggctca
     5161 tccacgttag cttttatatc tgatgatatt ccagatgatc caagagaaat cgtcgcgcgc
     5221 atcgaacatt ccagcaatga atttggattg ccgggatggg aacctgagcg cttagctcct
     5281 atcaaatcct tgcttgaagc ctatgcatca gtagacgaag aaaaacttcg agaaaatttt
     5341 gcctattttc tccaatcaat catcccaaca tgtgaagagg taggggtcaa aatggctgta
     5401 catccagatg atccgccata ctcgattttt ggattagctc gaatcgtaaa gaatcgagaa
     5461 gatttagatt ggttgtgtcg catagtagac agcccttata acggaatcac tctttgtaca
     5521 gggagtattg cagaagatcc agaaaatgat gtatatggga ttttagcaga attttgccaa
     5581 agagatcgaa tcccatttgc acatgttcga aatattaaat ttttagctaa tcgagatttc
     5641 tatgaggcac cacataaatc cgaatacggt tcattagaca tgtaccgcat actaaaaaca
     5701 atgtatgaca acggatttaa cggatacatc cgtcctgatc atggaagaat gatctggaat
     5761 gagacaggaa gaccaggata cggcttatat gatcgagcat taggtgctgt ttatctaaat
     5821 ggattatggg aagcaatcga aaaaggaaac aaaagtaaag ataattagtg aaaaaaactg
     5881 taattttatt atgtaaataa gaaggatgtt caaattcttg atacataaat ccggaggttg
     5941 ttaataagca ttcggattta tgtatcttat ttggtctagg aagtaacttg attttctttt
     6001 ttcgattgat ccatgagtgc ttcttctttt aattcaaaga tatattctga gatcctcata
     6061 atcacttttt tccagtcttc tggtgtgatg attcctttta aaatatagat ttttttgata
     6121 tgagaaggca attgtttcaa agggcagtcg ctgataacga tgttgcagtt ctccataaaa
     6181 tcattttgta ttttgatcga ttcatttgaa aaaatttgtt ctatcttaac gatcagatct
     6241 ttactgatat aaaaatcacg cgtatagttc acaccaatca caattcgcgg ctgttttttg
     6301 tagcgatcat acaaatgtac taggtcttca accatccatt gcacattttc cttttctaaa
     6361 ataactggta acaagtgttg attttgttct ctgaaatcca tataaaattg ttcaatggcg
     6421 gtttggactt ctttcaatac gctatgttct tgataaagca tgaaagtagg aaggggcgta
     6481 tttggttgat tgaagttaag aaatccatta taaagtttat ttcgtgcgaa tgccaatgag
     6541 taaagaatct tttctttttc aggtataaac aaatcaaata cagtacaaag ggactccacc
     6601 atttctcttg aataaagaac atgaggaacg tttgcttttt ttaaaagttt gtattgctcc
     6661 aagcttgttt cttccgattc gattttcgtg atagaaagtc gagctaaaat attcactaag
     6721 atgcgttgtt cgcgacaaat tggcagatta ggtcgcaaaa tatctacaga gagatcaata
     6781 aaaaagtcaa tatattcata ctctgtgaat tctttttgta cgtctttcac agaagttgca
     6841 ggaaaattct ctttacatat ttttaataga aaatataatt tatctatcat accgctattt
     6901 agtcggtcaa gtacttcttt tttaaaaaaa gtattcatca taaaagccac tacttgcgga
     6961 tcttcatcat aaaattctgt agcagtactt gaaaagatgt tccaataaat atctaatgct
     7021 gcatattgaa tttgtacctc ttttccagaa aaagactttc tgccattatt agaaaaggag
     7081 atagacaaac cgtactatgc taaaaatgca ttaatctttt tcattttcgt atacaaataa
     7141 gaggcactat agtttgtttg ctgactgaaa ctgataatcg aatttgtctg acagctgaac
     7201 aaagaagtca agatctgata actgactgat tcgttgcagt atcttccaaa tagacgataa
     7261 tagcagtaag ccggatcaat tccttcaaaa ttcaacttta aaaaaggttt ttgcttatca
     7321 atatgcagtt ctgttagatt gagacgctca aagtcttgtt ctaattcttt gatgtatagt
     7381 aaaaaatttg attcagacat atggaaatga tctgctaaat attgtgtagt aataaagaaa
     7441 cgatgttcag ataggaaaag aaaaatatct aattttgtat tgcttttttt cttcaagata
     7501 aacgttttaa tcgcttcttg agtttgcatg aaagaggctc ctttttactt aaattgttaa
     7561 aacttgaaaa tattactgtt tttttcataa atttagacaa aaaaacagta aacatatagg
     7621 tagaatctct actatcttat ttcttgcata caaaaaaatc aaggagtgag agagttatga
     7681 gatattcaac catcttaata tcaacatttg cagccttaag cttatcgctt actataaatc
     7741 agacagtcca cacagaggag acagaaacag ccaaatggac agaaaccaag tgggccaacg
     7801 tcaatcgaat tacctttcag gatgaatttt catccttcaa ttcaggagtc gcaaaattaa
     7861 gaaaaggaca aacagcgacc atcaaagcaa aaattgacta ttcgggagac ttagcaccta
     7921 tactacaatc agctgttaca tttgagaatg ttgatcccac tttgtcgatt ggctatgacg
     7981 agaacacttt gactatccaa cacaagcaag cgatatttac cttcaccgtt acattaaatc
     8041 aggacttgtt aaaaccagcg ctttttactg tcaaggtatc agatggacta ccgaacgatc
     8101 atcatttaac gccgtatagt cagcggcaga caattgagca agattcagcc atcgactcag
     8161 gaggagatct tgttgaagaa ccaacagata aaccagaaaa tgaaaataag ccagaagtac
     8221 cgcctacaga aaatccagac ggagaacaga aaccggaaat agaacctgga gaagaacccg
     8281 atacagaaac tcaaccagag ccagacaata aatcaaaacc tgaaataacc cctggagaaa
     8341 aaccagatgt aactccagaa ccagatacgg attctggaaa ccaaacagta ccggaaacaa
     8401 atccagacac agataatgaa actgaaaatc cagaaaaacc agaggtagat ccagaagaaa
     8461 aaccagatgt gactccagaa ccagatacgg acgcaagaga ccaaggaata ccagaaaaaa
     8521 taaacaaaaa aacaattcaa gaggatggaa aaaaagagag taaaaaatca aatctagcaa
     8581 ttctaaaaat aaatgaagag cagctgaata aaaaatcacg aatatttgat agcgcccaat
     8641 cagctgaaac cttaaaaagt agcaaggata caacctttgc tgtcctgaaa cgaaaaataa
     8701 acagctacca aaatccggag agtcacaaaa caaagttata ctgtggagcg gaatcattct
     8761 attaagtata gcaacaatgc tttctgcaaa aagatttaaa caaaacagat ctctctaaca
     8821 tttgaataat gtctgtgaac gcatttttca aataaaaaga aatatccaga aaatgacccc
     8881 aaaataccag attgaaatct gatatttggg gtcgttcata ctttatcttt cttcatattt
     8941 attttaaggg atatctgttt cttttggaag atggttcggg atctttttca ctttcaatac
     9001 atctaaaaag aaggtaaaga tcggaatgcc gacaatcaag ccccaaacgc caaagaaccg
     9061 ttcgctaaca agtaagacca caaatgtata aaaaatcggc agttctgttt tgctggacat
     9121 caacttcgga ttcagcacat aagactcaat tagatggaca atcaaaatcg taatcaggat
     9181 ataaacaaca tctcggatgc ctccttggga ataagctatg aaagatagag gaatacaaga
     9241 aatgataaca cctgctactg ggatcagact taaaatgaaa atcatgatcg ctaaactcag
     9301 cagctgatga aagccaaata cagccaaggc gattgtagtg agcaacgtat ttaaaagagc
     9361 gacaacaaat tgagcttcta ataccaaacc aaatgtattg acaaacttat cggcaaaata
     9421 gtaaatatct tgaaagaacc aagaaaattc actttttaaa aacaatcttg agaatacaat
     9481 tgtttttttc ttttcaatca taaaaaagaa acttaagatg aatgacatag caaacgccac
     9541 tgccattttt cctatatcct gtaaatagcc aagcgcaatc gaagcgccat tttgaagctg
     9601 ttcaaaaaaa tcgtttcgct caatataagt atggatatat tgtaaaacag gatctgcatc
     9661 ttttggctga tgctcgtaaa aattaataac ggactggatc atgtcaaatg tttgatgaat
     9721 aagcatcggg atatactttg tgaatgccag atagataagg taaacgatca gtgtataaat
     9781 catgatagct aaaacacctg tagggattcg gacaaatcgc tgaatgattt gaacaagatg
     9841 aatcatcaaa taagtaaaga taaatgtgag caaaatggtt gtaatcatac ttctaactaa
     9901 gaaaagaaca aaaacgacaa ataataaaac agtgagacgg cgtatttttt cattcgccaa
     9961 aaaacgctga taaacggtca aatcgacaac ctccaaataa caaaaaataa acacgttatt
    10021 cattatatga tggaatatga aaataagaaa gagcaaaaag aaaaaccgct aaatgacgtt
    10081 atcttatgag ttggttttcc tcataaaatc gttcatctag cagtttcagc cctttttctg
    10141 gtcttgatga aaattatcta gcatttttcg ggtcagatgt tgcagtgtat ccaattcttc
    10201 ctgttcaagt ggaagataag acttgatctt ttgtggaata ccttctgctt gtttttgcaa
    10261 cgcagcacct tgagcagtca gcgtaacatt tacgattctt tcatcacccg tttcacgctg
    10321 acgattaata taattttttt ctgctaactt ttttagtaag ggagtcagcg taccagaatc
    10381 cagacacaaa tcatttccta gctctttcac tgaagtcttt ttcttttccc ataaaactaa
    10441 catcacaaga tactgggtat aggtaagatt cagcggcttg aggaggggag tgtagtgtct
    10501 aatgatttct ttagaggcgg catataaagg aaaacatact tgattaacta aatatagatc
    10561 ttccattttt ccacctccgc tgttttacta tagtgtatca tagcggattt acacaaaaaa
    10621 gtctattcac taactgcttt cagagtaact tctatatttc cagctgtagc ttttgaatac
    10681 ggacacactt tatgcgctgc ctctaataat tcttgcgttt cttccagaga aagaccttcg
    10741 atagatcctt caatctctac acctaagaca aagctcgttt cactttctga ataaagggaa
    10801 acttgtgcac tgacagtaga agccccagag atattttttt gctgtttgat caattctaat
    10861 gcactgttaa aacatgcact gaaaccagct gcaaacaatt gttcagggtt ggttgcattt
    10921 tcgacgcgtt taccaggagc tgcaatattc aattcgaatg tacgatcggg attatgcact
    10981 tctccagagc gtccgcctgt gttgatcatt ttcgtagaat acattttttt cataaaatta
    11041 ccacctttaa ttatatttta tacaattgaa ttttacacaa tataattgtg gaaagcaaag
    11101 aaatggattg gcataagata aataagagaa agcacatgaa acagacgcgc tttctcgaat
    11161 agcaaacata tacaaaaaat atcagagaca aaaaataatc tcatcataaa cattacatat
    11221 atttctcaaa aagttgtttt ctttcttcaa aaattacttc acgatggacc tgatgaagca
    11281 tccataaata accaaaagga tctctgaaca ttgagttagt cacaccaaag tccatcattt
    11341 ctgtcatttc ttgaatctct acacagccat ttcgtaatgc tttattatgt gtgtcctcga
    11401 tatcagagac gatgatattg aaccagatcg tatttggatg ttcagcagta ggagcagtca
    11461 aaccaagaga cgtgttttca tccagcatat ggaatctcgt accatacaag gtaaaaatga
    11521 cttcattatt gcctctaggg taatctgttg cctcaatcag ttgtatttca tcaaagattg
    11581 tttgatacag ttctagtgct ttcaaactat catttaatac aaaatcgatt tccgttcctt
    11641 tatgcataaa gatctcctct ccttttcagt cgaacagtta ctagattctt ttattatacc
    11701 agctaaagaa aacgcattca aaaaaatagc ggatttactt accaactcat tcgattggat
    11761 gataagtaaa tccgcgtccc attacttctg ctacgctgct gacaatgaca aaggcacttg
    11821 gatcgatttc ttctatcaat ggtttcattc gggaaaattc atgatcagtc acaacgatca
    11881 ataaaacctc ttttgacttt cgattatacc cgccttttgc ctctagcagg gtaaggccac
    11941 gtccaatctc agcacttaag cgaaggcgga tgtcttccaa ataatgttca ctcatgacca
    12001 taacagattt ctttcgattg atacctgttt cgatataagt catgacaatt gaagtgattg
    12061 caatagataa aatagcataa agaaactctt ctattccaaa aataaacaga ttaagagaaa
    12121 caacgaatgc gtctgtaaaa aataagccaa tcgaagtatt caagtggaaa tattttttaa
    12181 aaatgagggg agggatcgtc gtaccgccgc tagaagagtt gtttttatat aaaatcgcaa
    12241 ctcctaaagc aaaaatcgca cttccaaaaa tgacagacaa caaccgatct gaagtaatca
    12301 ttgtttctgg aactattgca atgattaaag gaaaaaccaa gcttccgaaa agtactttaa
    12361 aaaagggttt tttacctaaa aatagaagag ctagtaccaa cataacgata ttcaacacta
    12421 aaataactat agctcggtca aagcctaaag cagcttccag taatattcct agaccgctga
    12481 cacctccagc agcaatatga tgaggcccta aaaacatatt gatagcgact cctaatatga
    12541 agagggcaaa aataatctta atgtatgtcc atataaattg tctcgtttct ttcatttatt
    12601 cacttcctta attcattaat aatactctta tcttattata acttactttt caccttaagt
    12661 taactttaat taataaaaaa attaggctag tctaaattat tatgtttcat tcttctagaa
    12721 tatacttcta cgccaaaaac tgttaaaaaa cttttacttt agaaaattgg agaaattgat
    12781 cgcttaagat aaattcagga cactaaatag tacaggaagt agactaaaac ttttaaaagt
    12841 tttaagtcta tctatttggc aagtttaaaa aataaaaatt cggtatcaaa aaactgttac
    12901 cgataacatt tttgaaagcg ttgtcttttg cttctctatc acttatagtg atgacgtcat
    12961 attaaaacac aaggaggaaa tcaatggata cagctgcaat ttatcatcga ccggaaagtg
    13021 aatatgcatt tctatatacg aatacaagat ttcgtattcg gcttcgtact aggaaaaatg
    13081 atatcaaaaa agtatatgtg ctatgtggag atccatatac cataacaact gaaaaatggt
    13141 atcaaaaaca acgaccaatg aaaaaatgtc taagtaccaa tgttcatgat tattgggaaa
    13201 ttgaagtgac gagcgaaaca agtcgcttac agtatgcgtt ccatgttgtt ggggaagata
    13261 acacggactg tttttacggt gatcaaggaa tttttcctta tcgagaaaat gtgattactg
    13321 aaccaaattt ttattttcga ataccttatt ttcatcaaat cgatcgattc actattccag
    13381 aatgggtaaa agaaacagta tggtatcaaa tttttcccga aagatttgca aacggggaca
    13441 aagcaaatga ccccgaacac actttacctt ggggaagtaa agatccagac cgagaagact
    13501 ttttcggggg agatttgcaa ggtgtgatgg atcatttgga ttacttgacg gaccttggca
    13561 tcaatggcat ctatttttgt cccatattta aagccacatc caatcataaa tacgatacga
    13621 ttgattatta tgaggtggat cccgcttttg gcgataaaaa acttttaaaa aacttgattg
    13681 atgaagcaca taaaagaggg atacgtatca tgttcgatgc tgtattcaat catatgggtg
    13741 tccattcacc acagtggcaa gacgttctca aaaacggaga aaaatcgatc tataaagact
    13801 ggttccacat ccgttttttc ccagtagatt cttatcagat gactgactta ccagaaactg
    13861 ctgaaaatat cccatatgat acttttgctt ttactccatt catgccgaaa ctaaatacag
    13921 caaatccaga agtgcaaaaa tatttgctgg atatcgcaac ttattggata aaagaattcg
    13981 atattgatgg atggcgtctt gatgttgcta atgaagtcga tcatcatttt tggaaaaaat
    14041 tcagagaagc agttacagaa atcaaaccag acatctatat ccttggagag atttggcatt
    14101 cctctcaagc ttggctgcaa ggggatgaat tccatgcagt gatgaattat gcatttacag
    14161 attcgattaa agattatttt gcaaaaaaga aaatcacagc ttcacaaatg gtcagtggaa
    14221 tgaatcatca gcaaatgctt tacagagatc aggtgaacga aggaacattc aacttattag
    14281 attctcatga tactgccagg atcttgaccc tttgccaagg taacaaagaa ttgatgaagt
    14341 ctgtgatggc ctttatgttt cttcaaaaag ggtctccttg tatctattac gggacagaaa
    14401 tagggatgac tggtgaagat gacccggatt gccgcaaatg tatgatatgg gagaaggagg
    14461 aacaagatct agagctattc ggttttataa aagaacttgt ttctctaagg aaacagttgt
    14521 ctaagattat ttctgaaggt tctactcagt ggctaatagt aaatgatcgt gaagataagc
    14581 tctatttcac acggaaacta gagggacaga tcatctatgt ttactttaat cagtcaaaag
    14641 aaccttgggt agtagaacag gaaaatgagg tgattcttag tcaaaattgt cagttgttag
    14701 aagacgggaa agcagagata cgacaatacg gatttttgat catgtgttga aaactgttac
    14761 cggtaacaac aagaaagcgt ttgcaaatga agatttgtgc ccattataat aagggtgtaa
    14821 cagcacgttg aatacaaagg aggaaatgaa gatgaaaaaa agaagtgtga aacagttggg
    14881 tttagggtta atctctttag gaatcttagg cggattagcg gcttgtggca atggtaactc
    14941 ttcagcagaa ggggaagccg atgaaggaaa aacgttaaca gtttccgttg attctggata
    15001 taaagattat ataaacgaaa taaaaaacac tttcgaaaaa gaaaacgatg tgaaaatcaa
    15061 gctagtagaa aaggacatgt tcgatcagtt ggaatcatta gcgttagatg ggcctgctgg
    15121 aaagggacct gacgttatga tggcagcata tgaccggatc ggtgcgttag gacagcaagg
    15181 acatctggcg gaagtaaaac tgggaaatga agcagcttac gatgaaacag acaaagccca
    15241 agtgacttat gatggaaaaa tctacggaga acctgcagta atcgagaccc ttgttttgta
    15301 ctataacaaa gatttagtcg atacagcacc atcaacgttt aaagatttgg agaatctatc
    15361 caaagacagt cgttttgctt ttgagtcaga agcaggaaaa aatacaggat ttttagctaa
    15421 atggactgac ttttattatt cctatggttt aattgcaggt tacggtggct atgtatttgg
    15481 tgatgatggc acaaatcctt cagatatcgg tttgaataat gcaggtgctg tagaaggaat
    15541 ctcttatgca acagattggt tcaaaaatgt ttggccaaag ggaatgcaag acataaaaag
    15601 tgcaggagac ttcgccagcc agcaattcat gtccaataaa actgcagcaa tcattgacgg
    15661 tccatggcaa gcgcaaacat acaaagaaaa caatatcaat tatggtgtag ccaaaattcc
    15721 tactctaaat aatggacagc cttatcaacc gtttggcgga ggaaaaggat gggtagtcag
    15781 caattatgct aaaaacaaag acctctctca aaaatggctt gattatgtaa cgaatcagga
    15841 aaatcaagaa aaattctttg aaatggtaaa tgaaatccct gcaaaccaac aagctaggga
    15901 aacagcaaag gctaaaaacg atgaattaac aacagctgtg attgaacaat atgaaacagc
    15961 tcaagcgatg ccaaacatcc cagaaatggg cgaagtttgg acaggtgcag aaaacttgat
    16021 gttcgatgca gcatcaggtt caaaaacacc aaaagaatcc gcagatgaag cagtaaaaac
    16081 aatcagtgaa gccatagaac aaaaatactg attaaaatga taaccgtcca tttttataaa
    16141 aaatggaaca ggtgcttcag ataaaagtgg caagagcgag atggaaaatt atcttgctct
    16201 tgttgccatt atcttaaaag cagtttgcgg tttagcaagt ttagaggggg cattcagatg
    16261 cagcaagata aaacaagcaa tatcaacgaa aaaaaaatca cgctttgttc acttattcca
    16321 gggatggggc agtttgtaaa tggccaagct tttaaaggta tcgttttttt cgccatattc
    16381 gtcctattca ttggtcagat gcttctgggc ggatttcatg cattggtagg attggttact
    16441 ttgggaagta taccaatgga agatcattca ttatttattt tgattgaagg cactttgcaa
    16501 ttcatcatta caatcatttt cttgattttt tacggtttga atcttttaga cgcttatcga
    16561 gtagcgaaga tgcgtcaaga gggcaaaaaa atcaatcgga cgatgaagga agtcatacat
    16621 aatataggtg atgaaggttt tccttatcta ttgacacttc ccgcttatct tctcatgatt
    16681 tttacgatca ttttcccagt cctagttaca ttgttcactg catttactaa ttatgacttc
    16741 aatcatattc caccagccag tttaattgat tgggtagggg ctaaaaactt ttttagtatc
    16801 ttctttttaa gctcatatcg taatactttt tttgcagtct tttcgtggac cgtgatttgg
    16861 actttatgtg caacaacttt gcaaatcacc cttggtattc tcacagcggt tgttgctcac
    16921 cagaaattta ttaaattcaa acgtattttc ggtatcattt tcttattacc atgggcggtc
    16981 ccagctttta ttactatcat gagtttttcg aatatattca acgatagcgt aggtgccatc
    17041 aatactcaag tgattccttt cataaaccag cttccttttg tagatatcag tgcaatctct
    17101 tggaaaacgg acccgaactg gacgaaagta gcaattatct tagtgcaggg ctggttagga
    17161 tttccctata tctatgtgat ggttacagga atcttacaat cgatttcaga agacttatat
    17221 gaagcagcaa aaatcgacgg agcaaatgca tggcagcgct tttcaaacat cacgcttcct
    17281 gctgtcttcc tagtggcagc gccaacattt attacacagt atacggggaa tttcaataat
    17341 ttctccatga tctatctgtt taacaatggc ggtcccggaa gcgtaggcgg aggagctggt
    17401 tctactgata ttcttatttc ctggatctat aaacttacga ctggaacttc tccgcagtat
    17461 tctatggctt cagcaatcac gttgattatc tccgtcatcg ttatctgtgt gtcattgctg
    17521 gtatttaaaa agacaaatgc gttcaatatg ggggattaac aatgaaaaaa tttcattcga
    17581 gtgctcgatt ccaaaaacga ctttctcact tttttactta cttttttctt ttaatactgt
    17641 cagtcattat catctatccg cttttgatca cagcaagtac tgccttcaag tcaggaaata
    17701 ttgcagcttt tagtttagat ctttcaagtg actggacttt aggaaacttt actcggctat
    17761 tcagtgaaac actttacggg acttggtata aaaatacatt gatcatttct gtcctaacaa
    17821 tgatcgtaca agtgactgta gtaactttgg cgggctatac ttatagtcgc tatcgtttta
    17881 tcggccgcaa atcaagtttg atgttctttt tagtgattca gatggtaccg actatggcag
    17941 cattgactgc cttttatgtc atggctttct tattagaagc gctcgataag tactggttct
    18001 tgacaatgat ctatataggc ggtggaattc caatgaatac ttggctgatg aaaggatact
    18061 ttgacactgt accaatagat ttagatgaat ctgctaaact tgatggggca gggcatttca
    18121 ggattttttt ccagattgta ttaccgcttg tcaaaccgat gctcgccgtt caagcattat
    18181 gggcttttat gggacctttt ggggatttca tgttagctaa gttcctgctt cgaacacctg
    18241 aaaatcagac tgttgctgtc ggcttacaaa catttatttc tgatgtgaca aaccaaagag
    18301 tctctctttt tgctgctggg gcaattttga ttgcactacc gatttgtcta ttattcttct
    18361 ttttacaaaa gaactttgtt accggcctga catctggcgg aacaaaagga taggagcaga
    18421 gtatcatgca aacatttccg ttaaactatt tttccgcatt acgtacgcca actcaagtgt
    18481 ttgcgggacg caaactatta tcatggccta aatttttctt gatatttgta tttttagttt
    18541 ccttgatggt catgcctgtg actctttttt atgccaatca gatccaagcg attccattag
    18601 aacaatttct atccgttcat tcgctgatcg atgaacaagg aacccaaaaa ttttctgaac
    18661 ttgaattatc tgaaactggg ttacaagcat cgcaacaaac gatagctgtg acccctgaaa
    18721 ttttggtagg tgtgtcttta tctgagaaac aacaatccga tcatggaact tttattgatt
    18781 ttgagaagga acaatgggtg atccaacaaa aggataaatc tggtatacgc cgctatacga
    18841 tgaattatag cccatctttc caaccagatt ctgtacgtac gcccgaagat ttccaacgat
    18901 ttttagaaag agaattttat gccagtaatc gtccaacaat cattttatct tatagtttaa
    18961 gtcttggtct tgtcctattt gtcatgacga gtctcatttt attcggggct tcctttttct
    19021 tgtggatgac aagaaaaagc caactttcat ccattcatac attcaaagag agtgcaaatt
    19081 tgatgctgaa tgttatggga ataggttcca tggcagcagc agttgtaggg tttatccact
    19141 ttgactttat cttgatgcta agtgtccaaa caacgataac tgtcttgctg cttttatggg
    19201 tttttgtcaa aacaagattc aaagataagc gtgtcgaatg acaagtttaa gcgatttatg
    19261 cgacaatgaa tgtgaaaaaa atgaaaaata aagtagtttt tgaaaaattt ctgaaaaaat
    19321 taaacttctt agttgattct tttctttttt agtattaacg taatagggta gtttttttaa
    19381 ggaggaaaat catgttagag aaaactttat acaatcaatt attttcacgt tctttctcgc
    19441 tccctgtcga agtaaattat tgggacggta caacgaaaca atacggagat acggacactc
    19501 ctccccaaat caaaatcaat atccatgaag aaatcccaat gaaagagatt acaagtaacg
    19561 catctttggc actaggagaa gcctatatgg atcatcgaat cgagatcgag ggaagtatcc
    19621 aagcattgat caacgatgct tatcagcaag caaacagctt tttacgagga aaagattatc
    19681 taaaatggct gccaaagaaa gaaaaacata cgaaacaaaa aaacaaagaa gatatccatg
    19741 cgcattatga cttaggtaac gacttttata aactttggct agatccaact ttgacttatt
    19801 cttgtgctta tttcaataca cctgaagata cgttggaaca agcacaaatc aataaagtcc
    19861 atcatatctt agacaaactc tttatcaaag aaggcgaaac gttgcttgat atcggttgtg
    19921 gctggggaac attgatgttc actgctacca aagagtataa tgtcaaggca actgggatca
    19981 cactaagtga agaacaatac gatttgatta ccaaaagaat aaaagaagaa catttagaag
    20041 ataaatgccg tgtattatta atggattacc gcgaactaaa aggcgaaacg tttgatcata
    20101 tcacaagtgt aggtatgttc gaacatgtag gttctgaaaa tcttgaaggt tatttcaaag
    20161 tggtaaaaga tttattgaaa ccaaaaggaa cagctttgat ccatggaatc agccgacaac
    20221 aaggcggtgc gacaaatgct tggatcaata aatatatttt ccctggcggt tatgtccctg
    20281 gtgttactga attagttgat aatattacaa aaaacgattt gcaactgatt gatttggaaa
    20341 gcttacgtcg tgactaccaa ttgacattag aacattggac aaagaatttt catcaagtcc
    20401 aagatcaagt cacgaaagaa atgggtgatt ccttctatcg aatgtgggat ctatatttac
    20461 aagcttgtgc agcttcattt gaatcaagta atatcgatgt gattcaatat ttgcttgttc
    20521 aacctggaaa caatgacatc ccaatgcatc gtgcataatt gaggcaaaag tactgtttaa
    20581 agactattta aaaagaagct aagttttatg atgctccaga agtgaacttt tttgtctaac
    20641 ttctaggatt gtctctaaag cttagtttct ttttctttat attttcataa taaaagtttg
    20701 gttctttaat ttttaaacat gacagaaaat tttttcttcg ttatacttgt aatagaatac
    20761 tattttataa caaaggggat cgaatcaatg aaaaaaactg ttatttatac tagtttattt
    20821 gcagctttgt ttttagctgc ttgtcaatca aataccaatc aaggcacatc tgggaatacg
    20881 tcttccagta gcgaacagcg ctcttcacag gtatcggact cctcaacaaa agcaagcaca
    20941 tcttcgtcta gccagacaaa aagttctagt gaagcaagta ctcagtcaga tatcaaagtg
    21001 tctgcagctc gtgcgatcga attgttccaa gataaatacc cagatactgc aattactagt
    21061 ttggaattag atagtgattg gggatcttac ttctacaaga tagaaggtgt cgatgatcaa
    21121 aaagaatacg aagcaagaat tgatgctcaa aatgaaaaaa tggagacaga gaatccagaa
    21181 actctagatc gagatgaaca aaatggtcaa aagaaaaaag aagatggtct ggatgttgat
    21241 aaattgatca cgattgagga agcagcaaaa atcgccgtcg acaaagtagg aagcggtacg
    21301 gcaacagatt gggatttgga taaagattta ggtactactt attgggaagt caaagtaaaa
    21361 gacggcaatc aaagtacatc agtcaagatc aatagccaaa caggcgaagt actgtcaact
    21421 gaacaagatg attaagaatt tcttcggaaa acaagagttg atttttcttt tagtcatgat
    21481 acactagaca aaataacttg tttgaaggag cgatttactt gagtttagaa acgacctttg
    21541 ccaatcatat ctttgccaat cctttgatga atgcatctgg tgtccactgc atgactaccc
    21601 aggaattaga tgaactggct cattcagaag caggagcatt tatcactaag agctgtacga
    21661 tcaatgaacg aaaaggaaat cccgaaccac gctattttga tgttccttta gggagtatca
    21721 attctatggg acttccaaac ttaggttttt cttattatct agagtatgct cttgcgtatg
    21781 aaaaagtgca ggaaaaccaa aatcaaccgt tatttttttc cattgccggg atgagtgtcc
    21841 aggaaaactt ggaaatgctt gagaaaatag aaaaaagtgg gtttaatggg atcactgaat
    21901 taaatttatc ttgtcccaat gtgcctggaa aaccacaact agcttacgat tttgaggcca
    21961 cttatgaaac actgaaagaa gtattcagca tcttttctaa gccgctagga atcaaattgc
    22021 ctccttattt tgattttgct cattttgatc agatggctga tatcttgaat cagtttccat
    22081 tgacctatgt caatgctatc aacagtgtgg gcaatggttt gtatatcgat acagaacaag
    22141 aagcagtagt catcaaacca aaagaagggt tcggcggcat tggcggagag tatatcaagc
    22201 cgactgcttt ggcgaatgtt cgcgcttttt atacacgttt gaaaccagaa atccaaatca
    22261 ttggtacagg tgggattcgg acaggacaag atgcatttga acatctgcta tgtggcgcaa
    22321 gcatgttaca gattggtacc gagcttcata aagaagggcc ggaaattttt tctagaatca
    22381 taaaagaatt aacgcagatt atgtcagaaa aaggatatac gtcaattgac gaatttaaag
    22441 gcaaattacg caccatttca tagcaaagag acgaaggtca tgattgatct tcgtcttttt
    22501 ttataaaaaa cactcgtctg ttcattcctg tgcaagcgta ctcattgtat gttagacgaa
    22561 cttgtgggaa aataacagag gcaaaaagga ggataatatg aatgatgcaa cagaattagc
    22621 aaaagagctt aaagaaaaag aatttagttt tcaagaacta gttacggata tacagaaaaa
    22681 aataaagaag caaaatcatg aattgaatgc gtttgtcgcg cttgaaccat tcgagagtaa
    22741 ttcccacgag ctattgattc cccagtcaga aaaagggcca ttggctggta tcccatttcc
    22801 tttaaaaatg ctcggtcaag aaaaaaaggg atggctagct acttcaggat ctcgtttatt
    22861 tgaaacacac cgagcttctc atacatctaa ttatgtgcgg caagcagaag cgataggtct
    22921 agttcctttt ggacaaacca atgcgccaga gtttggtttc aaaaatatta cagacccagt
    22981 catctatggc ccagcaagaa acccttggaa ccttgaatgt acaccgggcg gatcaagtgg
    23041 aggagctgct gctgcagttt catcaggaat cgttccttta gctggtgcta gtgatggcgg
    23101 tggttctatt agaatacccg cttcgttttg cggattgatt gggctgaaac cttcgcgagg
    23161 gacgatgcca gttggtccat atgcttggag aggatggcaa ggagctgcta ttgatttcgg
    23221 cttgacagtt tctatgagag acacggaggc tttattttat gggatgagaa gtatccattc
    23281 cggtgcgcca tatcaagtat cgccagttga gtggcaaaca catccaagaa aaaaacggtt
    23341 gaagatagct ttttgtgttg attcaccgat ccactcacag attagtgaag aggcagtact
    23401 tgctgtaaaa aaagctgtag atttccttgg aaaagaaggg catgatttga cagagattcc
    23461 ttacccacta gatggtcgta aactgatcac gtcttattat cagatgaatg gcgcagaaac
    23521 agcagcaatg atccgctcga ttgaaacagg catccagcga gctgtcagaa aagaagaact
    23581 tgaacccatt tcttggactt tgttccaata tggtcagaag ctctctgccg ctacttacat
    23641 cgattctctg catgtatggg atgaagcagc aattgtcatg gaaaacctac atcaagaata
    23701 cgatctattc ctttcaccaa cgactgcatt taccgcccct aaaatcagcg aagatttgca
    23761 aagtgatgcg ataagagcac agatggaaca catcagtgat tgttctgaag tagaaggttt
    23821 agacgttgtt tcttccatgt ttgaggagag cttgaagatc acaccttata cacagttggc
    23881 taatctgact ggacaaccag caatcagctt gcctactcat ttgacaaaag aaaacatgcc
    23941 tctaggaatc caatttatgg ctgccagagg aagagaagat ttgctatttc aaatcggcta
    24001 tctatttgaa gcaaatcatc agttccattt accaaatcca tggaaatagc agaaacaagg
    24061 agaagaagaa tgaagaaaaa aagattgttt gctctaataa ctagtgcctt ttttatttta
    24121 agcgcttgcg gtacagctgc cgctaatcag gaagataaca gctggagtaa tgtagaaaaa
    24181 aagaaagaat tagtagtagc gacttctggc acactgtttc ccgcatctta ttataatgat
    24241 caaaatgaat tagttggtta tgatgtagat ttggcaaaag aggtggctaa acggttaaat
    24301 gtcaaaatct cctttaaaga atacaacgtg gatggacaaa tcacttctgt ttcaaaaaga
    24361 gaatcagatt ttgctgcgaa cgattttggt atcacaaaag aaagagaaaa aaaattctcc
    24421 ctttcagaga ctttcaaata ttcatttgac agtatgatcg tacgaaaaaa agatgattca
    24481 ggaatccact cacttgaaga tcttaaaggg aaaaaagcag ctggcgaacc caatactagc
    24541 tatatgcgtt tagcggaaaa acacggagct aaattagtca catatgacaa tgccacgaat
    24601 gatcagtatt taagcgatgt agcaaatggt cgaacagatg ttattctaaa tgattattat
    24661 ctgcagaaaa tggccgtatc tgctttacca gatatccctg taaaaatact ggagaacgtt
    24721 tatttcaatg aaaacgaaag tggtttttta ttgaaagaag gaaatgatgc tctgaaaaag
    24781 aaaatcgatg gcgtattaaa agagatgaaa gaagatggga cgacaaagaa aatatctgaa
    24841 aaatattttc aaactgatgt ttcagtcaaa ccgaaagaaa cgatggctga gtcggtgagt
    24901 gagtgatgta ctttccccta attgattttt ccctcatgtg gacttcactt cctttcgtac
    24961 tgcaaggctt ggtatacaca ttagggattg gcttcgtgtc gtttctttta ggaaatttgg
    25021 tagggttgct tttgacagtt ttaggtcttt tggattggct gcctctgaat gtatttattc
    25081 gtttttattt atcgttcttt agaggcattc ccgcactagt cctgctgttt ctgttatatt
    25141 tcgggttacc ttatcaatta agtgccttaa cggcttcagt catctgcttt acgattacca
    25201 gcagtgcatt tattggtgaa atctatcgag gatcactagc aggcgtttct tcaggtcagt
    25261 gggacgctgg tttagcttct ggtttttctt tctttggggt attgaaatat atcgttttgc
    25321 cgcaagcttt tcgaatctca gtaccggcac tcagtaacgt agctatggat ctagtgaaag
    25381 gtacttcgtt agcagcaaca atcacagtgc cagaaatatt tcaaaaagca aagatcatag
    25441 gcggtcggac attcgattat ttttctctct atattttagt tgcactgatt tattgggtca
    25501 tttgtatcgg gatcgaaaaa gtgcaacagt atttagaaaa acgttctatc catcagtcaa
    25561 tctgaaaaat agaaatggtg ctagcgtggc tggcaccatt tctatttttt gtttcttgta
    25621 tattctgcct agtattttaa aaccatttca tgtaaaatgt aaatcgaaaa tatgttcgga
    25681 ggtagaaaac atgaagaccc cgctaaccat cgaaatagaa tcctcgttat accaatattg
    25741 catcgagcaa ggcggcttgg ttgtagaaga agtcacgatg cctgatgacc aaggaattgt
    25801 agatacgctt agctgcttga caaaatttga tggaagccga gaatggcgat gctatgagct
    25861 gaaagtgaca aaagcagatt ttcgctctac agccaaactg tcatttgttg gacattataa
    25921 ttattttgtt ttaccaagta aactatatga agaagtaaaa actgaaatcc ctacaggaat
    25981 aggtgtttta gtgtatcgtc cgtacacggt tgaagaggag atgcctgcct ctggtacatt
    26041 tctgattgcg aaaaaagcag caaagaaaga gcttgctgta ccggaagaag ctttgacaaa
    26101 ccgcttcatg gcttctttat tcagggaagt aagaaaagca aagcaaatgg agcgaggcgt
    26161 aaaatatttt ccaaaagatc aattgtacaa agaattgaaa agacgttcag aaaatcaaga
    26221 tccattttct aacgaacgat attatgaacg gtttttagag gaaatagcta gtcaggcaat
    26281 caatgagttg caagaagaag ttatcgcgct tcagcatgat tatgaatatt tgaggcaaca
    26341 acagcagatc cgtcggttgc ctacagagcc attggagtga aaaatatgta ctatccttat
    26401 ttaagaggaa aacaatttga cctattagca ttaaaagaag cactgtcgcg aggattgctc
    26461 tccaataaaa tccaaccagt gatcgaacca gtacgtgatt ccgctacgct taaaaatgtg
    26521 atcgaattat ttcaaaaaaa gcaccatcca atcgctgtga tccaaaatcc ccaagtagga
    26581 caattcaaat tattcgatca gcatgtgcac agttggacag tcaaagaaaa tagctctgtc
    26641 gtccctgcac agatcttaac gccagaaaat ctcagtgaag tactggattc acctccagcg
    26701 tttcttgttt tcgatggaca gcactaccca aaagatacag aggtgtggaa acagttggca
    26761 ggagttgatt cgaaattttt gataccagat actagtcgct ttcgcatctg gctgcccgaa
    26821 aacaaaattg tgatccgtga ctcttttcaa acgagaaagc atgttgaaag ttatgcagat
    26881 aaaacagatg acttttttag tgatgactat ctttttttcc acgcagacgg atatattgga
    26941 ttttctgatt tcacgatcga aggtagccgt tatttcgata aagggttccc tagccgagcg
    27001 cttgctatcc atctaacgta tattgatgct tacggcaata tacgcgtgaa acattttgtc
    27061 tctgacagta acgactctgc taaagaccaa gcattgaagt ttcttgaagc aggagataaa
    27121 atgaaaaaat ggatcatgcg tcaccatcat caactcttga ttacttcagg aatgattgaa
    27181 ttattggctc tttaccagca acaaaaattc cctggactgg gagtgttgaa aaagtggtca
    27241 cttatgcatc atttggaact tattagtcac ctgctagatc atccaaaaga ttggcttaaa
    27301 tattattcaa aagttcagga actatatgaa gtgatccaat gattagataa tgtaaatgaa
    27361 aaaggaatag aaaagaggaa actggatgta tattcggcaa acaaggaaag aagaagtaga
    27421 agaagtggta gaaatcatta atgccgcagc ggatttcatt gcttcgcaag gctctcctca
    27481 atggcaaaac gggcagaaac caacaaaaga aacgattttg aacgatattg aaagaaaaga
    27541 aagctatgta ttaatagtgg aagatcaaat tgctggaaca gctgcattag tctcaggcgt
    27601 ggatcccgtt tacacaaaaa tagatggtag ttggaaagaa tcaactggtc cttatttatc
    27661 gattcatcgt gtcgctttag cagaaagtac tcgaggaaaa aaactagcaa aaccgttctt
    27721 agaacatttg ttgacgattg cagccagcaa aaatatccaa gatatccgta tcgatactta
    27781 tccagctaat gaaccgatgg aaaagaccat ctattctgct ggattcactt atcaagggat
    27841 gattgaattt ccttttgaac acggcgaaag aaaagcatat caaataatca tcaattgact
    27901 ataaaatcat ctgagcaaag ataaagacat taacactact gaaaaaaaca atcatatgat
    27961 agccaagagg ttgtaacaga agtggccagc ttcaaaaaac aagaaagaat ttccgaaaat
    28021 gcttctcaaa tttttgtgga attttagcgt attttacgaa atagctgctt tctgaaactc
    28081 ccttcatcga gaggtcgtga catagctttt gtcacgatct ctcggcttat cctacgagta
    28141 ttattcgata catttatatc aataatcctt tttttatttt atctgcagct tcttggaacg
    28201 ctccatcgtt tgacacgact aaaagtgagg ggatagcttt caaacgtttc aactgtgcta
    28261 aatcttctac ataaaccgcc agtctggctg aaatctgttc agatgaatct ccacgttttt
    28321 tcaggcgcga ttgcaatgtt tctttagaga tcgtaataaa gataggacgg actaccgtcc
    28381 cgaaatactc atataaatgc caaaaaccat ttgctgtcaa aacggcgtaa caatttccta
    28441 ctgtcagttt ctttttaatt tctgctttac tggaaccata tttatgttga gcatattggt
    28501 cccactctgc aaactcattt cccttgatca tctgattgaa tgtattgtca gaaacaaaat
    28561 aataatcctc ttgatctttt tccccaggtc ttggtgatcg attcgtgtaa gtaatgatct
    28621 tttgttccgg ctgaaaaata gcttctgcga gtgaagtttt tccagaacca gaaggaccga
    28681 taaatacata gcaataattc attgtcattt ctcctttgat gattgaacaa gtgaaaaagc
    28741 tttggaaaat atttccaaag cttttaaaag taactttgct tttttgtatt ttataccagc
    28801 aagccagtca caatagcact taaaaagctg actaaagtcg ctgttagcaa aattttcaat
    28861 ccgtgttttg cgacaagatt tcctttttct tcatttaatc ccttcatcgc accagaaatg
    28921 atcccgatag aagagaagtt ggcaaatgaa acgaggaaga cagatataat tgctgttgta
    28981 cgctcagtaa aatggaaatt accatttgct aattctgtca tagcaacaaa ttcatttgta
    29041 accagtttag tcgccatgat actaccggca tccactgctt ctttccatgg gattccgctg
    29101 atgaaggcaa gaggggcaaa gatgtaacct aggatctctt ggaaagtgat gccgaatatc
    29161 ccgtggaaga tagcattgat catggcaatc aaagcaacaa aaccgataag catagcagca
    29221 accgtgatcg ccacatggaa accatcaaga atgtattctc ccaacatttg gaaaaatgtc
    29281 tgttctttgt tttcttcaat gatcagctca tcttcttttt cttctaatac atagggattt
    29341 acaattgaag ccagaatata accgccaaat aagttcaaaa caagggcagt gatcacatat
    29401 ttagaatcga tcagtgccat ataagatccg acgatcgaca tagaaactgt agacatagcc
    29461 gatacactca tggtgaacaa acgtttctca gataggaagg gtaattcctt ttttatggaa
    29521 ataaaaactt ccgattgtcc taaaatagca gaagctactc cattataaga ttccaatttg
    29581 cccatcccgt tgattttact taatcctaga ccaacagctt tcataaataa tggaagaatc
    29641 ttgatatagc gtagaatacc gataatagca gaaataaata caattggcat caatacggaa
    29701 agaaagaaag gtgcacttcc tttatttgct acaccggcaa aaacaaagtc aacaccttgc
    29761 ccagcaaaag caagaagatg atcaaacatt tttgccaata cagaaacaac cgctgtacca
    29821 aaagttgtac gtaataatac gaaaccaaga acaagctgta aaacgaacat cacaattagt
    29881 ggtttcaatt tgattgcttt tttgttactg ctgatcaaaa aagcaagccc taatgtgaca
    29941 aataaaccaa taatacctaa taaataactc aaataatcac tcctattcca aattccgttt
    30001 tattttacct gttttgcaaa taaaatacta atctttagtg aaattttcat tactttttta
    30061 gagtgaaacg cattcatttt ccattagaaa ataaagcgaa caaaataaaa ggtttaaaaa
    30121 gtgtaacgtc ttagtaaagt ctataaaaaa acgaaactat cgattaagct aatagtttcg
    30181 ttttcaacat ttataagtaa actttttcta ttggtaatat agattttcgg aagggtagac
    30241 aggtgcgcag gtaatatcga ttcctagttt tttcaatgtt tgttcatcat ttttattgac
    30301 catcacagta gagtgggctt gtgctccctc aagttcagct agtttatcat aagcaacttg
    30361 agcgattgga ttcgttactg cactgattga taaagcgatc aagacttcat tgatgcttag
    30421 tgcatccaaa gaggaatgaa gtgtctttga tttcaatcct tgtattgttt caagaactaa
    30481 tggtgacaat aaaggaatgt catctgcgat atgggctaaa tgttttactg catttaagat
    30541 aactgccgct gacgcatcca tcaatgaact tgtacgcccg gtaatgatga cttcatctgg
    30601 taattcgatt gcaatcacag cagaaggatc ttttgtagga ctttgcgctt gaatcttatc
    30661 tgcatagtca tgggcaggga ctaccacgtt tcggtcttca ggacgtaaac cgacttcttc
    30721 cataatcagt ttgattcgat taactgcttc ttcgtcgatc aatccttttt tgaagtcgca
    30781 ttctgtttgg aaataacgac ggatgatttc ttgtttagag gcttcttgga caacggtatc
    30841 atccacgatc ccaaatccta cacgattcac acccatgtca gtaggagatt gataaacaga
    30901 ttctttacca gtaatttttt caataatacg tttgattaca ggaaatgtct caacatcccg
    30961 attatagttc acagctactt tattgtaggc atcaaagtgg aaggaatcga tcatattgac
    31021 atcttttaga tcgactgtgg ctgcttcata agcaatgttc agcggatgtt tcaacggaac
    31081 attccatact gggaaagttt cgaacttgga gtaaccagct gcatgtcctt tttggctttc
    31141 atgatacagt tggttcaagc aagtagcgag ctttccactt cctgggccag gagctgtcac
    31201 tacaacgatt ggtttagttg tttcaatata ctcatttttt ccaaaaccat cttctgagac
    31261 gattttttct acgttgactg gatagtcttc gatttctgag tgtttataga ccttgatgtt
    31321 tcgtctttct agtttattga taaagacttt cgtggcaggc tgtccactat agcgagtgat
    31381 aacgacactg ttgattaata agccataacc acgtaattca tcgattaacc gcaagatatc
    31441 catatcataa gtaatcccat aatcaccacg gatcttatta cgttcaatat ctccagcata
    31501 gacgcaaatc agtatttcag cttgatcttt caatttttgc agcaatttga ttttcgcatc
    31561 ttcttcaaat cctggaagta cacgtttcgc atgcttgtcg tcaactaact tcccaccaaa
    31621 ttctaaatat aatttgtcat agtggttcac tcgttccaaa atatacgctg actgctcttc
    31681 aatatatttt tctgaatcaa aaccaatttt tttcactagg ttccctcctt aagtactaaa
    31741 gcattttttc atttttttga ggattcgtca actggaagag cgaaattcct caaaaaaaca
    31801 aagaaaatgg tttaaaccaa cttgtctaga tagttattca ttggtatgat agtttttgat
    31861 aaggaaggtg ctacatgaaa aaaagacaga aaaagaaaaa cgcatataaa cactatatcc
    31921 gttcgatttt taccggttat gaaagaatgc ttgaagatcc cgaattggaa caattgacgt
    31981 ttacttatct aaacgaagaa acgcagctaa ctcgtgatga gcatcagcgc attcatttca
    32041 caacaagaga tctgcctagt aaataaaaaa taagataaag gtcatcggat tcgattctgt
    32101 cctatcagat ggaaagacta gttgatcgaa aaaaaggctg taccaaattt tggtgcagcc
    32161 tgttttatgg agcattgccc gctctttttt ataacaattt tttacagaaa atggtactat
    32221 tgaagtatta ataagtgaaa aacagatgag taagaggagg caagtagtat gaaagagcca
    32281 gatacaaatg caaattatga gccagacgaa ctagaggaga cgaacgcgga tcaggataca
    32341 ggacaagaat cgtcttcttt ttacgatatc aaagaaaaag caagcatgaa tctttcccaa
    32401 ttattagaag acttacaggc ttttgaaaaa gctgtccgcg aagaagatat ccctgagatc
    32461 taccgtttat acaatggccg attgaatgaa gaattaaaaa gaagctcaaa tgaaaatcac
    32521 gagatcgatc aattgctgat gcgtaagatc catgatcgtt ttttacagga atttcctttt
    32581 atggaacaag tagaaaacat ctcgccaacg atgagctatt acaaaatcgg cacttattac
    32641 catgatcggc ccacggtcgg tattgatgca agtctgccag aaatttttgt tttgcctaag
    32701 atcgatgaag agtgggagaa atattcccaa ccggagtcaa cggactatga caaaaaaatc
    32761 aatgagctag acgcaaaggt gatcactgct cagacagaaa ttgaacgttt agatgaacaa
    32821 ataaaagaaa tcaatcgaca aatgactgat ttgaacgaca ataaaggatt cttgaatcgt
    32881 aaaaaaatcg aggaagaaat tcaagaatta gagaagaaga agcaagtact atctaatgaa
    32941 aaattaggct ggcttcctta tattgagacg cctgaaacaa tccagcagca aaaagaagca
    33001 ttgaagcaag aagcaagagc agaccagctt cgtgcagcca tcgtagaaaa agaacaaagg
    33061 caaatcagac gctatttcgg tagcaaagag ggattcggtc aagcaatcca tgaattcttg
    33121 atgaattatc ttggcaatga gaatcctaat aatcaatcta atgaaggagg ttccgagtat
    33181 gaatgaacac gatgaagcag aatggacaaa aaatgaagaa caaacagaag atcatgaaga
    33241 aacgaacact tcattggaag aacaaacaag taatcctctg gatgacacag aagaaatccc
    33301 tatgctagag gaaacaaaaa tacatgaaaa agaaaaagta actccgacga atacgaatac
    33361 tgcagaaaac acccatgctt acgaaataac tgaaaatccc gggtatttag aaggaaaaga
    33421 gcaagatcat caagatatcg tcagtttgat tgaaacagcg gaagcagaat tagcggattt
    33481 acgtatgaga aaagatttga tcgaatcaga ttttccacaa ttattagaat catatgatcg
    33541 ttttatattg aatgaagaac caatctccac gatcggaaag acaagcctga tcgaatactt
    33601 cacgtatgaa ttactgaaac cgttgaatga tattggtctg gaacggtcaa attcagctta
    33661 tgacacatgg aaaacaagac atttttcgat caattatacg ttgaatggac aaaacgaatt
    33721 agtcttttcc tttgtattac cgaccatcga tcaacgatac caagtggaat cgatgagtct
    33781 cctaaacgta tcaccagaga caatggaaat caatgtccaa gatgaccggg tgctttcttt
    33841 gatccgacac tggtcagtgg atcgagtatt ctctgcaggg cagatcacga tattcaatca
    33901 caaattgaat caattattag cccatgcaag aacacttggt tttaccgtta atcagacatt
    33961 actagacaat acgaagccac ttcgtttgaa tcttcagagc gaatttgaat tgacgaatga
    34021 agtattggat gatattttta tcgtggcaat gaaaaatcca tcctacgata tggaaaagat
    34081 caacgaaact acttatcaag tacgattaga taaaggacag tctttaacga taacaacaaa
    34141 cgaaaaagat caaacagtct tgtcgatctc atctggagac tacccacgat ctgtcattga
    34201 tttctttatc aattacgaat tcttggtccc attaatggtg agaaagtcct gattggataa
    34261 gttggattac cagatgtcat tcagataatt ctgttccaaa ggacttgcgg cagttatcca
    34321 gaataattgt cacaagtctt ttctgtatgt actaaatgga cgtgaataac ttgctgatga
    34381 aggagggatt tcattaggaa tgtccacaaa taaaatagca cagcttagct tattgtctgc
    34441 tgcttgtgta gcaggtagga tcgcttttca atttattccg aatttccagc caatgactgc
    34501 tattttttta tttatagtac tttacttaag cttaaaagat gcgttaattg tcatgagtct
    34561 ttctattgcg atcagcagtt tttattttgg tgttggacca tgggtcatcg gccaatggat
    34621 cagctatact gttgttctga gtttgttttc aatcgtctgc ttgagaaaaa cagtgcaaag
    34681 gaatctatgg ttaaagggag tctgtttttt tctggcgggc atggtttacg gaataggtat
    34741 gaccgttttt gatacgctgt tatatcgttt gcctcaacct tggatctatt accttcaagg
    34801 ggtgtcgttt gatttgatgc acagtatagg caatgttgtg ttctttcttg tttttctgcc
    34861 agtcgtcaaa cgtttttaca atcattcagg aggaaaaaat gaaaaaaata atttatagtt
    34921 tgcttgcaat gagtgtcagt atactcattt tgacaagttg cagtacaacc tatacacaat
    34981 cggcttccaa ccaaaaggag gaagtaaaaa catcgcaaaa agcaacaatc acgttacaag
    35041 aaaatggtca aaacttttcg gaaaaagaag tggtcttcaa aaaaggcgat gatttactga
    35101 gtattttgaa acgaaatttt gaagtcaaag aagataacgg cttcatcact tcgcttgaag
    35161 ggcactcaca ggatgagaaa aatcaactct attggatgtt tactgtggac gggaaaagtg
    35221 ctactactgg tgccaaagag atcaaattga atgatggaca atcggtcatt ttcaatttgt
    35281 ctaaactata agtttcataa aaaactgtta aagggcgaat attgccataa ttcagtgagt
    35341 tcagttgatg aaagaaggaa aataacatgg aaaattttaa aaaaagcgga atgttttccg
    35401 taattgctgc tctaggggct aatattctag ttgctatcag caaatttgtc ggctatgcaa
    35461 tcagtggcag tgccgccatg ctaaacgaaa gcatccacag tatcgttgat tgcagcaatc
    35521 agattttgct gttattcggt gataaacaag ccaataaagg acagagcgag ttgcatcaat
    35581 ttggtgaagg acgagcaaaa tactttttca gtacaatcgt cgcaatgatg cttttcttcg
    35641 gcggtggggc acttggggtg atggaagctt ttgaaaagtt gacgcatcca tcccatgaag
    35701 tgggtaatcc gatcattgtt atagcaatac tgttatttgg tttggtaatc gaaggaagtt
    35761 cgttacgcgt ggcaatgaaa gaaatcaaag aattgaatac tgaaaaactt tccacgatgc
    35821 gctttttgcg cgaaagtcga cacagtgaaa tactgattat ttttacggaa gacttgtgtg
    35881 ctgtaatcgg tttggtcttg gctttagcag gaacaatatt gacttatgca accgggattg
    35941 ctgcatttga tgcgatcagc ggattgctga tcggtttctt gctgatggga gccgccattt
    36001 tcctagcaaa agaattttat agtctgatca tcggtgaaag cgtgacagca agcgatttat
    36061 caaaaatcaa gttagctttt gaacgtcctg agatcagtcg gctgatcgat ataaaaacag
    36121 ttcatttaag tccaacggag attttagttg cagcaaaagc agatatcgtt ggtaacaaag
    36181 agaatgaagc ttactcggtc atcaatgata ttgaaaaaaa tatgcgtcag tctcttcctg
    36241 acaagaagat gtatatatac attgaaacag ataaatatga cccaaattat tctcgtaaat
    36301 aaaacgttta ttcatgaatt gaagcaaaaa tgagaaaaat ataatcttgt ttttaaataa
    36361 aacggctaca atttagcgaa tttatgttac aatgagtccg ctgtttattt gttgtaatta
    36421 aaggagatat tttaatgaac aaaaagaaaa gtgcgccatg gcttcaagct ttgcatgcag
    36481 tgatgccact ttgtataagc tatataccag taggattagc ttgcggagta ttgttgcaaa
    36541 aagttggatt tgatcctctc ttatcaggac tgttatcgat tctggtcttt tccggtggcg
    36601 cgcagttttt agttgcatct atgcttacga cgcaagcatc atttgcaacg acattattga
    36661 tggttttctt tttagaatta cgctatacat tattaggagc aagtcttgcc ggttttatga
    36721 aaaaagaaaa gcggacgttt ttggctgtct tttctcaatc actgaatgat gaaaactatg
    36781 cggtgaacta cttaaagttt tcaactgacc cttcctggaa taaaagaaag gctttatatg
    36841 tcaattggtt ctcaatgact gcatgggccg gtagcaatat gatcggtaat tttttcggct
    36901 cggtgattcg agtagatgct gaccttgtcc attttgcttt gacggccatg ttcattttca
    36961 tgtttatcat gcagatgaaa agcgcactac tgatctttac aggtttattc tcaggcgtat
    37021 taggggtcgt attcatggta ttattccaaa atactttcgg attgattgca gcaacagtca
    37081 ttgcttcatt tgccggattt atgctggaga aggcattcaa aaaagaaaaa gctaagaaac
    37141 taagagaccg cggtaaagat gtcaatgagc cgctcttcaa cttcccggaa caggaggcca
    37201 atcaccatga ctaaactata tttgctactt ttagttgctt gtctgttcat cgtggcatat
    37261 atcccgcgct tgttcccgat gctttatttt actcatcgaa aagtgccatc ctggttcagt
    37321 gactggatga aatatgtacc ggtggcatta tttgcggcgt tagcttttaa agatgttttc
    37381 attactcatg aacatctgga tattgcttgg aatatcaaaa tagctgcaat gattctagta
    37441 gcaggcgtgg cttacaaaac acgttcgatg gcgttgtcag ttttgacagg acttgcgtct
    37501 gtctttttgc tgtctatgct ttaaactgtt ttattttatt aaaaacagag attgcgacat
    37561 aagttgttca aactgatcta tccgaactgc atttgcaaca gtcaattctg gatgcaagtt
    37621 tattcgtagg gataaaaaag attagtgttg gcttatgatg caagctcttt tttattttag
    37681 gaattagaga gggatttgtc cttctatgat tttttgctat aatagggaag atgtttattt
    37741 tatgaaagaa tagttgaggg aatttaaatg aataaaaaat tgatgcaagg aacattttgg
    37801 ctaacctttg ctaatcttct atgtaaagtt ttaggggtag tctatttgat tccctggcta
    37861 agtatgatgg ggaacaatca agatggtatg cttgctacga cattgtataa tgttggctat
    37921 cttccttatg gattgttcct gatgcttggt acagtcggat ttccaaatgc gattgcaaaa
    37981 aaagtagctg tagccacaaa agaaggagat aacgctgctt gtcggctgat ttttagaagt
    38041 acgatcaata tcatgtttgt catcggtatc gcctctgctg cactgatgta tttatttgct
    38101 cctttactag ccggaatcag tccgattgct aacgttaata atgggatact agctatcaga
    38161 agtttatgcc catctttgat tgctatccca attcttagtg caatgagagg atatttccaa
    38221 ggaaaaaatg atctgcggcc ttatggaact tctttgatta tcgaacaggc tgtgcgagtg
    38281 atcgttatct tggctggtac ttattacttg cgcgtattga ctgacggaac catattagaa
    38341 gctgtattga tcagtacagt cgcttctttc ttcggtgggt tagctgcgat tatccatatg
    38401 tatatcgtcg gacgcagaaa agacttcttc gaacttaaag attttttgat ttccagccgt
    38461 tatttcgaaa gagaaaaccg atctgcctct gtctctatca ttcgggaaac actaccgttt
    38521 atttttgtcg gctcagtcat tactatcgtt caaatgatcg accaagtaac gatgaaacca
    38581 ctgcttcact ttttccgtcc agagatcgca gatcagcaac tggaattctt attcagccgt
    38641 gcttctgtca atccaaataa attgacattg atcctgatct ccatggtagg aacagtagct
    38701 ataagcagct tgccaatatt aagcacgatg aagaaaaaag atcggataca aatcgaaaaa
    38761 acagtaggag atagtttttc gattgcgtta ttgattcttc ttccttcatt gactggaatg
    38821 tctttattag ctggaccact atacacattg ttttttggct atgatcctga aagtgtcgga
    38881 tatttccaaa tggctttgct agcttctttg tttttctctt tattcacgat cctatctacg
    38941 atgcttcaat ctttgaatca ccatcgtttt gcgatccgat taactgtaga agcaattatc
    39001 ctgaaagtca tcttccaagt aatcggatta ggattagttg gcggatacgg gatgagctta
    39061 tcaaatggct tagcattcgg tattgtcttt gttcgcggct atcactttat gtgcaaagaa
    39121 taccgaatag cacctttagc aaagatcagc aattttttct taaaaacttt ccgctccacc
    39181 ctgattatgc ttgcagtatg ttttgccgtt ttctttttgc tgaatcagcg attgtcaatg
    39241 gaatcaaaaa gctatgcaat gatttattgt gtggctgtag gaagtattgg cggcttagta
    39301 tttttatttt ctcaattcgg aaaaaatggg tttcgaatgt tgaaaaatgt taaacatcat
    39361 agcagaagca aagaataggt taactaatcg ttttttgctg tcacttgaaa aacggcaact
    39421 cgtatgatta cgcttgtcgt ttttctattt tccaatttta gtttcatatc taatcttact
    39481 tatgatatag ttataaggtc tggaaaaatt ctttgaaacg aggaaattaa acacatgaaa
    39541 cttgcagtag ttacagatag ttcggcatat ctgccggaac gtatcaagaa ccatcccgac
    39601 ttattcgtta ttccaatccc tttgattatg gatgggaaaa tttataacga aggaatcgat
    39661 atcgaagcgg atgaatatta tggactttta aaaaacagta aagattttcc tactacttca
    39721 caacctgtcg taggggaagt tttgggttta tatgaggaat tgaaagagaa agggtatgac
    39781 acgattttaa gtatccatct ttcttcaggg atttctggct ttgtcaacac attatttgcg
    39841 atgaaagatg acatcagcgg agtaacggtc attccttatg actctaagat cactagtatg
    39901 ccgatgggac acatggtaga agctgcctta gacttaaatg atgaaggtca gtcccttgat
    39961 gaaatacttg cacatatcga tcgaatccga gataatacct atgcttatct gattgtagat
    40021 gatttgaaca atttagtacg aggaggacgt ttgacaaatg gtgcagcgct gattggcagc
    40081 ttattaaaaa tcaaaccgat cctgacattt tctgaaggga aaattgtttt attcgaaaaa
    40141 atccgttcaa gtaaaaaagc atttgctcga gctgaagaaa ttattggtag aagaaataac
    40201 gaaatacaac cgccagtcaa actttacgtg atacatgcaa ataatttaga agtagcgatt
    40261 gcggagaaag agaaattgca gaaaaaatat ccaaatgcag aaatcgaaat tggtcatttc
    40321 ggaccagtca ttggtacaca tttaggtgaa aaagccatcg gcattgccat ttcagcgcaa
    40381 taattccttt tattcaaaaa acataccaaa aata
//
LOCUS       DARBET010000020        38980 bp    DNA     linear   BCT 07-NOV-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4027
            SAMEA114569841-rid20112183.denovo.020, whole genome shotgun
            sequence.
ACCESSION   DARBET010000020 DARBET010000000
VERSION     DARBET010000020.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA114569841
            Sequence Read Archive: ERR12203666
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 38980)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 38980)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001972618.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001972618.1
            Genome Coverage       :: 132x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/07/2023 13:18:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,041
            CDSs (total)                      :: 2,975
            Genes (coding)                    :: 2,908
            CDSs (with protein)               :: 2,908
            Genes (RNA)                       :: 66
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 67
            CDSs (without protein)            :: 67
            Pseudo Genes (ambiguous residues) :: 0 of 67
            Pseudo Genes (frameshifted)       :: 26 of 67
            Pseudo Genes (incomplete)         :: 28 of 67
            Pseudo Genes (internal stop)      :: 20 of 67
            Pseudo Genes (multiple problems)  :: 6 of 67
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..38980
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA114569841-rid20112183.denovo.020"
                     /isolate="VRE4027"
                     /db_xref="taxon:1352"
                     /country="Denmark"
     gene            82..453
                     /locus_tag="SCJ86_001125"
     CDS             82..453
                     /locus_tag="SCJ86_001125"
                     /inference="COORDINATES: protein motif:HMM:NF016724.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase family protein"
                     /protein_id="HEG1168701.1"
                     /translation="MWLSDEKIQWRSYQKRILTNFATYLNNNSVHLVAPPGAGKTFLG
                     IEMIRRLDCKTLILVPSLILKKQWLTTIEEYFLVNPADSCYLSGAIEHPSHITVETYQ
                     TIYNRLKEEPTYGDQLELLVF"
     gene            633..1055
                     /locus_tag="SCJ86_001126"
     CDS             633..1055
                     /locus_tag="SCJ86_001126"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347586.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEG1168702.1"
                     /translation="MVKEEVLCPHQDFLYLIPANQETTQHYFTFLEKQNELVEALMES
                     QETVDYLLSLEFVQHPLEHSKEIYENFELFTSALIYLNHHNYELIPEHWQLLGSSKQQ
                     GKKASVPSLGKKELITIYQQMFEDQPELPIFYQLKKAG"
     gene            1200..1445
                     /locus_tag="SCJ86_001127"
                     /pseudo
     CDS             1200..1445
                     /locus_tag="SCJ86_001127"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347587.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            1554..2510
                     /locus_tag="SCJ86_001128"
     CDS             1554..2510
                     /locus_tag="SCJ86_001128"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347588.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEG1168703.1"
                     /translation="MLILANQASTFVQTQQLRGRGLRLGAQNKVTNIWHLAIGLPGVP
                     LSEQPDLKRMLRRLGNISGLTFESSPKIESGGDRFALPLEYTTTTMETYTQSMFYFSC
                     ERSRLKEQWSTALADGWHLSMPIVVRKVDQVKQKQETSLPIVVKNIEPLSFWSAIFKG
                     NLLEYFQIRRRQKLWEKECARKKSLAICVQTTLQRLGEVSDRKVIVAADQQEFRCYLE
                     NAGYREGQLFTEAMRELLAPLENPRYLIEGKKIVLTVPERFGRNKQIANLFLNELNQS
                     SLPKCQLVYTRNPEGRQKLVTYRMRQVFQTRDEVIIQKNIWT"
     gene            complement(3346..4017)
                     /locus_tag="SCJ86_001129"
     CDS             complement(3346..4017)
                     /locus_tag="SCJ86_001129"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347591.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HEG1168704.1"
                     /translation="MTIKTSLQNQAYQSIRQQIIYADLEPGKKVSEKKLEETLSIGRT
                     PIREALIQLRHQGLVDTIPQSGNYISLIDLDLAKNARFVREHLERQIMLECCAKMNNQ
                     KKQVLETIIAEQEKAVSQKNARSFFHNDNLFHKACFEIAEREEVWQWLEDNNTHLERF
                     RWLRVKTEGLKWEKIMEQHHQLFKALTMQDPEEANFLTSLHLHLMLDEQNVVLTTHPK
                     YFKNI"
     gene            4334..5965
                     /locus_tag="SCJ86_001130"
     CDS             4334..5965
                     /locus_tag="SCJ86_001130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannitol dehydrogenase family protein"
                     /protein_id="HEG1168705.1"
                     /translation="MVLIKDWQDSIEGLNKMQVQLPYFNVEAVKQEGQKHPVWLHFGG
                     GNLYRGFHAEIAQCLADQNELESGVVVCETFDEEVVEKAYHDYNNDILEIVMGEDGEL
                     KKRLLAVTADSLYCNPKNITDYQRVVKYFEDPTLQFTTFTITEKGYSLKDSKGQFFSV
                     VEADIKSGPTNPQHTMSIVTSLLYSRFNAGELPIAMVSTDNFSQNGQRFQDSIFMIAE
                     AWQKKGYVSEKFIAYLKDKEKVTFPWSMIDRITPNPSESVKMDLLKAGFEDLDIIHTA
                     KNTNIAAFTNTEKVHYLVIEDSFPNGRPKLEAAGVIMTDRETVDKADIMKVTTCLNPL
                     HTALAITGCLLGYKSIQAEMQDDDLVGLIKGIGYVEGLPVVESPGVIDPKQFIDEVIN
                     KRLPNPYIPDTPQRIATDTSQKVAIRYGETIKKYVEKDGNAENLTFIPIAIAAWLRYL
                     LAVDDQGKNFVPSPDPLLVELQEKFSVLKLGEQDPAKIHCAVQEILENKEIFGLDLYQ
                     VEIGKKIEKIFCEMLVGPQAVRKTINKYITEVGGK"
     gene            5968..7047
                     /gene="uxuA"
                     /locus_tag="SCJ86_001131"
     CDS             5968..7047
                     /gene="uxuA"
                     /locus_tag="SCJ86_001131"
                     /EC_number="4.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347593.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannonate dehydratase"
                     /protein_id="HEG1168706.1"
                     /translation="MEMTFRWYGANEEKISLQEIRQIPAMKGIVGTLMDIPAGEVWPR
                     ERIKALKDEVEANGLTLKVIESVNVHDDIKIGLPTRDQYIENYKQTIRNLAEFGIEVI
                     CYNFMPVFDWVKSDLDYHLDDGSSTLAYISKNIPTDPEEIIRKVENSTNNFELPGWEP
                     HRLSQIKQLLEAYKEVDEAKLRENFAYFLKAIIPTCEEVGIKMAVHPDDPPYSIFGLP
                     RIVKNRDDLEWLCNVVDSPSNGITLCTGSIAERPDNDVYEILSEFCQRDRIPFAHVRN
                     IKYLSDNGLDFYEAPHKSEYGSLDMYKILKTMYDNGFNGYIRPDHGRMIWGETGRPGY
                     GLYDRALGAVYLNGIWEALEKGASK"
     gene            7121..8548
                     /gene="uxaC"
                     /locus_tag="SCJ86_001132"
     CDS             7121..8548
                     /gene="uxaC"
                     /locus_tag="SCJ86_001132"
                     /EC_number="5.3.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347594.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucuronate isomerase"
                     /protein_id="HEG1168707.1"
                     /translation="MFLDKNFLLENEIAKRLFHKFAEHQPIIDYHCHLDPACLLENKQ
                     YKNLTQVWLNDKGAGDHYKWRLMRANGIEEELISGAGNDYEKFLAFVKTVEKAPGNPI
                     FEWSHLELQRYFDIDLTISEKNAPEIWRRANEKIQTDSYRPKELLKMMDVKAICTTDD
                     PTDDLRYHTELSKQDNSFKVLPTFRPDKVWSIKSSGFSNYILKLSQAAGIPIKNFNGL
                     KQALSQRVDYFHQIGCRLADHGLNTYFYEIADEKILDNLLIKALAGKTDFHLSEIAQF
                     VTGIQLHLMSEYTRYGWTMQMHLNALRNNSSILKEQIGVDVGGDSMGDQASLTQNVVA
                     LFDTAEKSGCLPKTILYSLNPTDWLPLATAMQSFQGGMIQKIQLGCAWWFNDTAEGMR
                     QQLTTMAQQSLLSNFTGMLTDSRSFLSYPRHEYFRRVLCQLLSEWVKAGRIPDDEMYL
                     SQIVENICYRNAYNYFNFFEIESRK"
     gene            8632..9279
                     /locus_tag="SCJ86_001133"
     CDS             8632..9279
                     /locus_tag="SCJ86_001133"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353537.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional 4-hydroxy-2-oxoglutarate
                     aldolase/2-dehydro-3-deoxy-phosphogluconate aldolase"
                     /protein_id="HEG1168708.1"
                     /translation="MKKVEILSKLEAAGVIAVVRGKNKDEAIKAINAITAGGIKGIEL
                     TFTVPNAEEIIKEIAKKYADQKDVIIGAGTVLDAFTARIAIMAGAEYIVSPSFDRQTA
                     EICNLYQIPYLPGCMTITEIKEALTAGVEIIKLFPGSAFGPSVVKTYQAPLPYVNIMP
                     TGGVSLDNLEEWFEAGVVAVGVGGNLLAPVEKGDFEEVTRIASKYIDKFYEIKNY"
     gene            9408..10784
                     /locus_tag="SCJ86_001134"
     CDS             9408..10784
                     /locus_tag="SCJ86_001134"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEG1168709.1"
                     /translation="MERKFGLRDKIGYMFGDFGNDFTFIFASSFLMIFYTKVLGISGA
                     TVGTLFLVARFIDAVTDITMGRIVDGSKPSKTGKFKPWILRMSGPVALASFLMYQTSM
                     ASASMSIKVIYMYVTYILWGSIFYTSINIPYGSMASAITSVAEERTALSTFRSIGATL
                     AGMFIGVLTPIFIYTRDSSGAQIVRGGSTFTIVAGVFSVCAVICYVICYKLTVERVKS
                     DESQLKVSLLKSLKGLAKNRALLSIIAAAIFLLLAQLLTMSMNNYVFPDYYNNAQGIV
                     LMNFINPILMLVLVSPVSLIFSKKYGKKELASVGMFFSAIVYLLLFLIKPSNMYVFLV
                     LTSVGYMGLGIFNTVIWANITDVIDDQEVKSGQREDGTIYAVYSFARKIGQALAGGVG
                     GWTLSIIGYDSLAKVQSGEVLQKLYNSSTLIPAVCFFVVGLILFFVYPLSKEKVLENY
                     NLLNQKHN"
     gene            10795..11589
                     /locus_tag="SCJ86_001135"
     CDS             10795..11589
                     /locus_tag="SCJ86_001135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HEG1168710.1"
                     /translation="MNSWIFCIDSDGCVMDTMTYKHNLFFGPIAAEIFDVHHREKFLQ
                     DWNNINLYSETRGINRFLGLVKTLEKNDVPSINRLKHWVSVTKELSNPSLKREIEYSQ
                     SEDLLKALKWSEEVNRQISQAKGLDKPFLSARDTIKALKKYGKIIIVSSANKEAIQEE
                     WERHELLSLVDELCCQDKGKKEDIIRSVIENGCDLDKILMIGDSPGDLEAANKNKVFF
                     YPILVNKEKESWENLRTDILFKCLSGEYIDIEQKYIDTFWKNLTNK"
     gene            11602..13368
                     /locus_tag="SCJ86_001136"
     CDS             11602..13368
                     /locus_tag="SCJ86_001136"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003056564.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-hexosaminidase"
                     /protein_id="HEG1168711.1"
                     /translation="MTIKVDLRKKPYHLDEDAISWIEETIDAMTLEEKIGQLFVNMGS
                     SRDKNYLSSMVNQYHIGAVRYNPGTADEVYDQNYILQTESKIPLLIAANTEAGGNGAC
                     TDGTEVGVEVKIGATNDAKYAYELGRVSGIEASAIGCNWSFAPIVDINRNWRNPIISS
                     RSFGQSPDKVLELSLAYMKGIQESGILPAAKHFPGDGIDERDQHLSFSINSLSTEEWD
                     NSFGKVYSGLIEAGLPSIMAGHIHMPAYSKHFNLDLKDEDLLPATLSKELITDLLRGK
                     LGFNGLVVTDASHMLGMTGAMKRSELLPTSIAAGCDLFLFFNDPDEDFNYMMEGYYKG
                     IISEERLRDALRRILGLKAKLGLHKKEKNKILLPKQEAMEKIGLIENKSIFKEVADQA
                     ITLVKDKQDIWPIVPEKYKRILLVDVHGVKGGFGEVVAGNRPQAIDLLKQILSAKGFE
                     VEKYISPEEELKGLSQEEKIKNIAGIYAAKRPIKDLTEKYDLIINIANVNPGTVQRIV
                     WPASKGTPDIPFYIHEIPTIFVSVQCPFHLADVPQVKTYINTYDGKQETLEILVDKLM
                     GLSEFKGISPIDAYCGLKDTFI"
     gene            13396..15204
                     /gene="uidA"
                     /locus_tag="SCJ86_001137"
     CDS             13396..15204
                     /gene="uidA"
                     /locus_tag="SCJ86_001137"
                     /EC_number="3.2.1.31"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017767467.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-glucuronidase"
                     /protein_id="HEG1168712.1"
                     /translation="MLYPIMTETRQVIDLSGLWRFKLDTNDDLQEELALEPMVEKESY
                     PISVPSSYNDLFETENIRNHVGWVWYEKEVILPKKLLEERLVLRFGSATHEAKVFLNG
                     KLINQHKGGFTPFESEINQFIKDGSNRITVAVNNVIDDSTLPVGILKETIQEDGDIKK
                     ENLVNFDFFNYAGINRPVKLYTTPKDYIKDVTINTKIIDSDANVEFNIDTEWTGEILI
                     AIYDEEAQLIAEAKGKEGVIQLKNPTLWEPLNAYLYSMKVSLINENKVIDTYTEEFGI
                     RSIQVSNGKFLINNKPFYFKGFGKHEDTYINGRGLNEAVNIKDFNLMKWIGANSFRTS
                     HYPYSEEIMRLADREGIVVIDEVPAVGLHLNFMATLLDDNVEKHDTWKEIRTENAHKM
                     AIKELIERDKNHPSVVMWSIANEPDSDSEGAKEYFEPLVELTKELDPQKRPVTIVTYL
                     KSTPDICKVGDIVDVLCLNRYYGWYVSGGDLKHAKYLLKKELDGWMERCPDTPIMFTE
                     YGADTVAGLHDTVPTMFTEEYQVGYYEANHEVIDKCDNFVGEQVWNFADFETSQGILR
                     VQGNKKGIFTRDRKPKMIAHYLKKRWSSIPNFSYKK"
     gene            15376..15777
                     /locus_tag="SCJ86_001138"
     CDS             15376..15777
                     /locus_tag="SCJ86_001138"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010818872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HEG1168713.1"
                     /translation="MQQNYNEKSIQHQFDAYCKKVLRNETKSIRKRNINITDNEEPLT
                     NSNEGKYSQTHFDEQDVYFLFGMEILISDQKLSAAIDHLSEIRRKVILLYYFAGFNDT
                     EIGKILNMSTSGIWYQRKKAVEQLKMGYDLW"
     gene            15771..16016
                     /locus_tag="SCJ86_001139"
     CDS             15771..16016
                     /locus_tag="SCJ86_001139"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002360096.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HEG1168714.1"
                     /translation="MVDNPKKRQYPLVPLSIIQSALEEDPISIKMILKHYHSYIAKLC
                     LTNGFNEAGQFITYVDEYMLRQLEIKLIEAILKFKIN"
     gene            complement(16340..16561)
                     /locus_tag="SCJ86_001140"
     CDS             complement(16340..16561)
                     /locus_tag="SCJ86_001140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297343.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEG1168715.1"
                     /translation="MRNEIANITIIRETLQNHTANEISKHTGLNLSTIKKLKSGERSI
                     EKLNLHDAICLTEFGLKNNRKNVEINIWK"
     gene            complement(16723..16795)
                     /locus_tag="SCJ86_001141"
     tRNA            complement(16723..16795)
                     /locus_tag="SCJ86_001141"
                     /product="tRNA-Lys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(16760..16762),aa:Lys,seq:ctt)
     gene            16871..17101
                     /locus_tag="SCJ86_001142"
     CDS             16871..17101
                     /locus_tag="SCJ86_001142"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002358447.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HEG1168716.1"
                     /translation="MDFQDYEKIFESYPSILKITDLTKMLNIGKNKAYNLLKDRSIEN
                     IKIGARYKIPKKAVIEYILKIQDEGKNHAIIK"
     gene            17145..18314
                     /locus_tag="SCJ86_001143"
     CDS             17145..18314
                     /locus_tag="SCJ86_001143"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002383715.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HEG1168717.1"
                     /translation="MKLRANIIEKRGTLQVVVSYKDSNGKIRQKWKDTKLKKKGNKKR
                     AEKIRDEFLLELESQLAMECECANPNLLFYDYLLNYLEVSKKQVAFNTYVGYKHYVHN
                     RIYKFFYPKKIKLNRLKPYHLQEFYQYMLNDNCTANTVIHYHAFIRKALQEAVITELI
                     STNVADKVRKPKKKQFVSQVYNQQEILKLLDILSEEKLYLVVLLTAFYGLRRSEVLGL
                     KWSAIDFLNKKIMINHVIIENPENMSQLIKKDQTKNSSSYRTLPLVETIERALVNQAK
                     WQQDNRILLGDDYILEDNEYVFTMEDGRLMKPQYVTHRLSKLIKKNGLKKIRFHDLRH
                     SNASIMLDSGQNMKSIQEWLGHASYSTTANLYTHLTAGVKDRAAEALENAFGFKE"
     gene            18888..19457
                     /locus_tag="SCJ86_001144"
     CDS             18888..19457
                     /locus_tag="SCJ86_001144"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1836 domain-containing protein"
                     /protein_id="HEG1168718.1"
                     /translation="MEKMRQQLMEWGKELETFRLPHWEELPDLELYMDQVRTLVDRYL
                     SPVIQGEKHPLLTSSMVNNYVKLGLIPAPVKKRYNKEHVAFLLAITTLKQVLTIPEIK
                     EGILFQGKTVGIREAYNLFCDEQEAAVWMVSQLAQGKSHPQKFDQATPVEYIAVRAAT
                     LSFAMKLLAEKTIVLETEYLKEEKTNEKQ"
     gene            19444..20304
                     /locus_tag="SCJ86_001145"
     CDS             19444..20304
                     /locus_tag="SCJ86_001145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374830.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DegV family protein"
                     /protein_id="HEG1168719.1"
                     /translation="MKNNKIAILVDSGTDVPEELIKKYEMYMIPLKIIYKDQVYTDKI
                     DITPEEIYQRLPEEIPGTSLPDGESITEIFEQIKKDGYEKVLAVTISSGLSGTYNVVR
                     LIAQQQEELEAYVLDTKNIGIGAGFSAIQAAKWLEEGVEWDQLISNLNELVERTKVFF
                     NVATLEYLQKGGRIGLVASIVGTALKLNPIISCNEEGIYYTVGKARGRKKSLDKTVAY
                     VKEKIGNAKVFNLAVAHGDAKEEAIEMEARLKREFPQANQIYFGQISPALVVHTGPGL
                     LGVGVQVLEG"
     gene            20494..21198
                     /locus_tag="SCJ86_001146"
     CDS             20494..21198
                     /locus_tag="SCJ86_001146"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016625064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HEG1168720.1"
                     /translation="MKKVLVVDDEKPISDIVKFNLAKEGYDVYTAYDGEEALEKVAEV
                     EPDLILLDLMLPKMDGLEVAREVRKTYDMPIIMVTAKDSEIDKVLGLELGADDYVTKP
                     FSNRELVARVKANLRRGATAAKEPEEAAPAELTIGDLTIHPEAYMVTKRGETIELTHR
                     EFELLFYLAKHLGQVMTREHLLQTVWGYDYFGDVRTVDVTVRRLREKIEDNPSHPNYL
                     VTRRGVGYYLRNPEQE"
     gene            21203..23038
                     /gene="walK"
                     /locus_tag="SCJ86_001147"
     CDS             21203..23038
                     /gene="walK"
                     /locus_tag="SCJ86_001147"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628799.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell wall metabolism sensor histidine kinase
                     WalK"
                     /protein_id="HEG1168721.1"
                     /translation="MKRKVRFFRSVNFKIAITFILILLISIEIIGAYFIRGLERSTIN
                     TFIKDMNQTVESLATTISPELNRKDNADDEEVNANIKRFIENSATSDIIEIRVVDEKG
                     IIRGTTDVNEQSAVGKKNDYVDINDFTTKRYVALDNDDKRVNINVQPILSPTGDAVIG
                     ALYVKSNIEQKYSEINNTAVIFFTASLIAAFISMIVSVLVARSITQPIGEMREQAIRI
                     ARGDYSRKVKVHGQDELGQLADTFNQLAERIEEAQDTMEAERNRLDSVLSHMTDGVIA
                     TDRRGKVITINDMAVSLLDIKNEEAIGQSILTLLDIEEEYTLRKLLEDPNEMVLDRST
                     STLESDQMILRVDFSMIRRESGFISGLVAVLHDVTEQEKTERERREFVSNVSHELRTP
                     LTSMRSYIEALSEGAWKDEEVAPNFLKVTLDETDRMIRMINDLLNLSRMDTGNTQLQL
                     EYVNFNELVNFVLDRFDMMVGNQEKNYKIRREFTQRDLWVEVDTDKIIQVVDNIMNNA
                     IKYSPDGGTITCRLLETHNNVILSITDQGLGIPKKDLNRVFERFYRVDKARARAQGGT
                     GLGLAISREVIKAHRGAIWAESKEGKGSTFYISLPYEPYEEEWWE"
     gene            23035..24351
                     /locus_tag="SCJ86_001148"
     CDS             23035..24351
                     /locus_tag="SCJ86_001148"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337978.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEG1168722.1"
                     /translation="MRISEKVVRIGLILMVALSIYFSYAIWLSPAGKSSINVDETNTQ
                     MIESQSNRKTSEVFLPLHVTWHHSGMIQETNSENLVYRLQLIIDKARFGKLSEVVSGD
                     KSQFEQVKNLNSGFELAYNAPFLLREYKEAFDLDLDVSNLAKESENMYFTRIQFDQGN
                     KKIRFLNYSNHLVYEANISLNWTDINKELTAKDTKWTEMTTEPSTIDTQYATKHSIKL
                     KKYSYILSQQPYTLFRNAFFQKPDSVKNNDESNELIFYGGNETMSIHSETQVVDFRGE
                     LPEKKENASVFSESFPYISKLGSSLGNLRYFDRSDNTIDYRIFVEGFPVFGTDSRGQI
                     GVEVGGETNGSVQVNIQTSLNTIQVPIPSDEEVELASTNEIIQQLKAKGADSNKIGSV
                     IVGYTWQNVKETNRVVDLLPEWYVKYKDNWYSANDLLEKLPGLEAN"
     gene            24352..25221
                     /locus_tag="SCJ86_001149"
     CDS             24352..25221
                     /locus_tag="SCJ86_001149"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738649.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system regulatory protein YycI"
                     /protein_id="HEG1168723.1"
                     /translation="MDFKKIEWIFFVAFLGLNVFLFSSYHDAVYQENNVIRSNQTESI
                     QQRLASDDITYTGKISSENKQGYYLSAEQTNLSTSLANYRKAGKSGLLASGVFLDGDV
                     LTKSLSSSDAEKNVIDPDKISSSLEDFLSNKDNILFGNDYVYTKNFSHLEEEPLEIQA
                     SQEYEGIPFNDDTAKIVLSLEKNDNDYSITKYMQTHLSDIEQLREKTELYTEEEAINT
                     LYINNKIPRGSKILWRQLAYSCILKVREKNVYVPVWHVAIETSDKVVQIESVNAFSNT
                     IVTNNTVPKVEDQ"
     gene            25276..26085
                     /locus_tag="SCJ86_001150"
     CDS             25276..26085
                     /locus_tag="SCJ86_001150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736688.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HEG1168724.1"
                     /translation="MAEEKPAFKISILASGSSGNSLYIESEKKRLLVDAGLSGKKITS
                     LMAQIDRTPDQLDGILVTHEHRDHIHGVGVLARKYHLDVFANQQTWEAMEPLIGNVPV
                     EQKHLFEMGKVRTFGDIDIESFGVSHDAAAPQFYRFHKENRSFVMLTDTGYCSDYMRG
                     VIENADGYLMESNHDLEMLRMGPYPWNLKQRILGDRGHLSNEDGALVMTDVIGNRTKR
                     IYLGHLSKENNMKELAHLTMENVLKEKDFGVGHDFEIYDTDPDEATELFSI"
     gene            complement(26188..26817)
                     /locus_tag="SCJ86_001151"
     CDS             complement(26188..26817)
                     /locus_tag="SCJ86_001151"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294128.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-binding protein"
                     /protein_id="HEG1168725.1"
                     /translation="MKIALIGATGHAGSFILDEALSRDLDVTAIVRHPEKLPSDVPFI
                     QKDLFELTTTDLASFDVVVDAFNAPKGKEEMHQTSLSHLIEMLEGTDTRLIVVGGASS
                     LFLDTEKTTRMIDQVPKNAPFYPTAFNMYEGLLKLKQSKNLHWTYISPASLFDPHGKR
                     TGTYLLSDDYLQRNAASDSTISMADYAIALVDEILDEKHEKQHISVVSR"
     gene            26987..28279
                     /locus_tag="SCJ86_001152"
     CDS             26987..28279
                     /locus_tag="SCJ86_001152"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340257.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HEG1168726.1"
                     /translation="MKEWLDRAFLPMITSVVNLRPLTALRNGMTYTIPIIIVGSLFFL
                     FAHFPVDYIAQWIDKTGFSMYFTQVYQATFDVIAFWIVFAIAYAYAKEEDIDRLPVGI
                     TALSAYLLLIQPLNTQIDTVWTGAEGILGAVTTGLIIGWGYARLVRLAKGWKQREKIP
                     ENVWQSFTSLIPIIVIVTFTLIGSAVFQHLFRMTVVQGVWTYVQLPIQSFIDTLFGVL
                     LLGFFVPFLWLFGLHGSAMVNGLVSPVLQANSLANAEILASGQELTVANGGHIVTQQF
                     LDQFMTVTGAGLTLGAVFFMTVFAKSKKYRELGNLALLPAFFNINESIIFSTPIVMNP
                     MMAVPFIFAPVVSGLITYSALYFGFVPLFTAVQVPWTTPPILSGFLVGGVPAAILQGI
                     VLSISFFIYFPFLKKIDSANCKKERQTKNDGAAEKMND"
     gene            complement(28355..29017)
                     /locus_tag="SCJ86_001153"
     CDS             complement(28355..29017)
                     /locus_tag="SCJ86_001153"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CPBP family intramembrane metalloprotease"
                     /protein_id="HEG1168727.1"
                     /translation="MSLKKYSFLSIFCYGLIFISPLILASIGLVKSTSELITATAVMY
                     ILGAVVLAIFYFKQREPLAIESAVKKASAPKIVIYGLVGIFVALILQSIAVMLESVLF
                     GKATPSENTQNIIQMIMEAPAFILATTVAGPIMEEFVFRRSILGIISRYSNFWVGAVI
                     SSLLFAFAHNDGHLLIYFFLGFFFSLEYKATGRIWTSMITHVGMNTLVVLVQLAVQKG
                     LV"
     gene            29242..29526
                     /gene="groES"
                     /locus_tag="SCJ86_001154"
     CDS             29242..29526
                     /gene="groES"
                     /locus_tag="SCJ86_001154"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738650.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="co-chaperone GroES"
                     /protein_id="HEG1168728.1"
                     /translation="MLKPLGDRVIIEVAKEEEKTVGGIVLASAAKEKPQTGTVVAVGE
                     GRLLENGEKVPAAVKAGDQVMFEKYAGTEVKYEGKEYLIVAGKDIMAIVE"
     gene            29577..31202
                     /gene="groL"
                     /locus_tag="SCJ86_001155"
     CDS             29577..31202
                     /gene="groL"
                     /locus_tag="SCJ86_001155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736685.1"
                     /note="60 kDa chaperone family; promotes refolding of
                     misfolded polypeptides especially under stressful
                     conditions; forms two stacked rings of heptamers to form a
                     barrel-shaped 14mer; ends can be capped by GroES;
                     misfolded proteins enter the barrel where they are
                     refolded when GroES binds; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chaperonin GroEL"
                     /protein_id="HEG1168729.1"
                     /translation="MAKELKFAEDARAAMLRGVDKLADTVKVTLGPKGRNVVLEKSYG
                     SPLITNDGVTIAKEIELEDHFENMGAKLVSEVASKTNDIAGDGTTTATVLTQAIVREG
                     LKNVTAGANPLGIRRGIELATKAAVEELHNISTVVDSKEAIAQVAAVSSGSDKVGHLI
                     ADAMEKVGNDGVITIEESKGIETELDVVEGMQFDRGYLSQYMVTDNDKMEAVLENPYI
                     LITDKKISNIQDILPLLEQILQQSRPLLIIADDVDGEALPTLVLNKIRGTFNVVAVKA
                     PGFGDRRKAMLEDIAILTGGTVITDDLGLELKDATIENLGNASKVVVDKDNTTIVEGS
                     GEKEAIEARVQLIKNQIAETTSDFDREKLQERLAKLAGGVAVVKVGAATETELKELKL
                     RIEDALNATRAAVEEGMVSGGGTALVNVISKVSAVEAEGDVATGIKIVVRALEEPIRQ
                     IAENAGYEGSVIVDKLKNVELGTGFNAATGEWVNMVEAGIVDPTKVTRSALQNAASVS
                     ALLLTTEAVVADKPEPAAPAAPAMDPSMMGGMM"
     gene            complement(31291..32439)
                     /locus_tag="SCJ86_001156"
     CDS             complement(31291..32439)
                     /locus_tag="SCJ86_001156"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317848.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HEG1168730.1"
                     /translation="MLKVWVEQTKDGQFKFIERYVDPYTEKTRKKSTTLTSNSPQAWK
                     KAQKILDKKIKEALEDYNKSDITFGELYKEWYEYYKQHVKRTSYLKVPMMMKHVSKHI
                     SDDTIVRNIDETLINKIIEDMYTFGDLSLNYTKQTKTTLSVMLNYAIDRKYIQRNPAL
                     AVKIHPKKVEEEKRRLSMNKKYLEKEEIDQILKQLYSNPRRKLHGIIAEFLYLTGLRY
                     GELLALQMKDYEDGKISINGTLDYTSVKMDNAIKTTPKNTYSQREVQLPNRAKELIES
                     VIADNILAGRPTDPDQYIFISTSGTPLALHSFNAILHKVEEELELEKSLSSHIFRHSH
                     VSLLSELGVPLKAIMERVGHSDANTTLSIYNHVTKRAKQQVIDKLNSL"
     gene            complement(32541..33125)
                     /locus_tag="SCJ86_001157"
     CDS             complement(32541..33125)
                     /locus_tag="SCJ86_001157"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005876753.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4352 domain-containing protein"
                     /protein_id="HEG1168731.1"
                     /translation="MAKKVMGQDGKMYKVSKPFYKKVWFWVLAVILIIIIGSALNGGS
                     DSNKASDNGGEKVTKSSTSASSSKEEKSDTFYKIGDTVKVGDAEYTLNSVELTDERNQ
                     FEENQPAQVVKITYTVKNDGDSDIPVGTDVEVYGPDDKKSETYANENTMGSVAPGKQM
                     DVTAHFTLNQKGEIEIHFSPLVSFEKAAIFKATV"
     gene            complement(33252..33683)
                     /locus_tag="SCJ86_001158"
     CDS             complement(33252..33683)
                     /locus_tag="SCJ86_001158"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ImmA/IrrE family metallo-endopeptidase"
                     /protein_id="HEG1168732.1"
                     /translation="MYLKLKEMLSEYNLKVVYMEMKEPGFYYPKPRIIFLNENLYGET
                     AEAFHLSHELAHFTASHFEYSVLYDTSTTFHSKFETEADKIAILILLNIYIENELTDE
                     SQFNLEKFMEYYSIQNKLRYTCYAVCQCYFKKKYSYARQYV"
     gene            complement(33701..34021)
                     /locus_tag="SCJ86_001159"
     CDS             complement(33701..34021)
                     /locus_tag="SCJ86_001159"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296612.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HEG1168733.1"
                     /translation="MNTYEIIKELAKEKGLSIRQLEMNFGYSNGYLGSWKRQTPNSIE
                     LARLADYFGVSVDYLLGREERETPKHVDLSENDTVFSFDGKEISKETMRKAIAIAKAL
                     EENE"
     gene            34321..34461
                     /locus_tag="SCJ86_001160"
     CDS             34321..34461
                     /locus_tag="SCJ86_001160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286578.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEG1168734.1"
                     /translation="MEKTVSAKIEDLKLDILKQAKVAMEHAVDKEDSAMVAAIAEILA
                     HV"
     gene            34759..35016
                     /locus_tag="SCJ86_001161"
     CDS             34759..35016
                     /locus_tag="SCJ86_001161"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286679.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphomannomutase"
                     /protein_id="HEG1168735.1"
                     /translation="MNKEMSQTIKVQKMIDDLTHGIDSQADKIIKELQGQKVKDAKML
                     LKTINFEMNPTKRKLADVLEEKLASAINEQELLFETDTFNS"
     gene            complement(34987..35295)
                     /locus_tag="SCJ86_001162"
     CDS             complement(34987..35295)
                     /locus_tag="SCJ86_001162"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286674.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEG1168736.1"
                     /translation="MKPEFVITMLDGEKIKIFADTLVVGYDDASNIEEKDGMFYSSQI
                     YVNTLQGDIGVSTLATSRPLVGLIGFLSKATFFSLGDDIDSDETVIYKTSAVKSIGFK
                     "
     gene            35373..36119
                     /locus_tag="SCJ86_001163"
     CDS             35373..36119
                     /locus_tag="SCJ86_001163"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage antirepressor"
                     /protein_id="HEG1168737.1"
                     /translation="MNTPQIFNFEQNEVRTILVNDEPYFVGKDVADVLGYSNPQKAIR
                     DHVDLEDKTQNDSFTVNGTAVVLINESGLYSLILKSKLPSAKKFKRWVTSEVLPTIRK
                     TGSYSNVPQSFAQALRLAADLEEKNQLLEQQIAEYEPKISYLDTILSSTDTVATSQIA
                     ADYGMSAIALNKLLNELGVQHKVSGQWILYRKHMNQGYTKSHTSEIPKADGGTKVVMN
                     TKWTQKGRVFIYNLLIAEGYYPQMDLEEIG"
     gene            36134..36337
                     /locus_tag="SCJ86_001164"
     CDS             36134..36337
                     /locus_tag="SCJ86_001164"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010752202.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HEG1168738.1"
                     /translation="MTDIAEITQRDREKIKEYVESSKFLTYTMLAERFGISKSYLSLI
                     LNGKKTSAEANRIIDSIITMYEL"
     gene            36442..36741
                     /locus_tag="SCJ86_001165"
     CDS             36442..36741
                     /locus_tag="SCJ86_001165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318516.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEG1168739.1"
                     /translation="MKKPTLSELIEAAEKAVKPDDWYRQSLILEKFHGMSKTTLVEYC
                     KEMETIPEFAEGILRPGHSTTFIHYHTFIWFLKWKDANKYRVKSLSPSDVLKEAS"
     gene            36741..36923
                     /locus_tag="SCJ86_001166"
     CDS             36741..36923
                     /locus_tag="SCJ86_001166"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320917.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEG1168740.1"
                     /translation="MGKFNRALVFSAPLIIYALGLWGSRQALIGTIVYMVWIFMGLDE
                     AEHRAKKASREGLTKI"
     gene            complement(36929..37405)
                     /locus_tag="SCJ86_001167"
     CDS             complement(36929..37405)
                     /locus_tag="SCJ86_001167"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320916.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEG1168741.1"
                     /translation="MRKKLNYILTATLTIYIILFITLSFLSSIKKIPFITLNKNSILK
                     EFNNTDYIAFLKLFSVCVLISCVIIFIVAKTCWTMLNKNQRDVENIAIFLIKITSSIS
                     IIIPMTVTFSKEQFNVAATYLSFIALFSFVLPKRNKSNFDNKELDEVRNSSPKEQK"
     gene            37458..38183
                     /locus_tag="SCJ86_001168"
     CDS             37458..38183
                     /locus_tag="SCJ86_001168"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage regulatory protein"
                     /protein_id="HEG1168742.1"
                     /translation="MQELVILKNKEAVTTSLQVAESFKKKHKHVLEAIESIKRSVENS
                     ANVEDGSNFGQMFVEGNEPDSYGRSRRVYFMNRDGFSLLAMGFTGSKAINFKLKFIEA
                     FNEMEDVIRKNTVPQTIEDMMIYQLEEMKDVKKDVSMLKDTMRISGQQEFEIKQKGNM
                     KVMEVLGGKESRAYEEISKKVFSKFWSEFKRTFSIPRYGELPRKRFDDAVSFIEMWLP
                     ETAIRMEIDQLNRQQRLFGDENE"
     gene            38357..38698
                     /locus_tag="SCJ86_001169"
     CDS             38357..38698
                     /locus_tag="SCJ86_001169"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353384.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEG1168743.1"
                     /translation="MNKELSTLDQYLTDSEWGKSNIKETNNRKIRRNLLTNEELSCDQ
                     DDLGNFVSIWDHVYLIHLSKHSNKPEYIYVIEDGLIDALEEYNRDNLIDISYYGSGKK
                     YIAEMEAEFDE"
     gene            38691..>38980
                     /locus_tag="SCJ86_001170"
     CDS             38691..>38980
                     /locus_tag="SCJ86_001170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340820.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1071 domain-containing protein"
                     /protein_id="HEG1168744.1"
                     /translation="MSEIKGTTNFEKLFSRKLNKILKKKGNFDYLSWAHAWEIMKKND
                     PQATVTINEYKHYRVVSGTHQDFLVEEYKPFLMDETGTYVSVSVTVKGHTET"
BASE COUNT        13387 a         6001 c         7779 g        11813 t
ORIGIN      
        1 acacatattc ttgaagaaat cttttttttg ctgtattcat ttagattatg atagagaaaa
       61 tacgtgagag ggggacctgg gatgtggcta tctgatgaaa aaattcagtg gcgtagctat
      121 cagaagcgta tattgactaa ctttgctact tatttaaata acaattcagt ccatctagtg
      181 gctccaccag gggctggcaa gaccttttta ggaattgaga tgatccgacg tttggattgt
      241 aaaaccttaa ttttggttcc ttccctaata ttgaagaaac agtggttaac aacaatcgag
      301 gaatatttct tagtaaatcc agcagatagt tgctatcttt ctggggcgat tgaacatcct
      361 agtcatatta cagtggaaac gtatcagacc atttacaacc gactgaagga ggagccaact
      421 tatggtgatc agctggagct gttagttttt tgatgaagct catcatttaa aaaaaagctg
      481 gggtgaattg ttgattgact tgaagaaaaa acaaccgaat ctgttgacga tttccttgac
      541 agccacgcct ccgattgata gctctcagac ggagtggaag caatatattg agttaaaatg
      601 gcctgattga cgaagaaatt agcattcccg agttggtaaa ggaggaggtt ctatgtcctc
      661 atcaggattt tctatatttg attccggcta atcaggaaac tactcagcat tattttacct
      721 ttttagagaa acaaaatgag ctggtggaag cgttaatgga aagccaagag acggtggatt
      781 atttgctttc tttagaattt gttcagcatc cgcttgagca ttcgaaggaa atttatgaaa
      841 attttgagct gttcactagt gcattaattt acttgaatca tcataactat gaattgatac
      901 ccgaacattg gcagctatta ggcagttcta agcagcaagg taaaaaagct tcagttccat
      961 ctttaggtaa aaaagagctg atcactattt atcagcagat gtttgaagat caaccggaat
     1021 tgcctatttt ttatcaattg aaaaaagctg gatgattgtt tgatgagtcg ctgcatttgt
     1081 ttccagagta tccagacact gttggtagtc aggcgattcc tgagaaaaag caagcagttt
     1141 gtcaaatttt gttaaaagaa gaagttcagc tgaagcaaga actgacagcg gtcattttga
     1201 tggatcgtat ttgcaaggag gtattagcag gcagtgatgg taccttagct tatggcgtgg
     1261 ttcctttgtt tgatgaagtt gttcgtctat taggtaacac tcagcaaatc ggtgccatct
     1321 gtggagagtt cttactgtta ccgaaaatgc tagtaactgt tgagctggca gaactagcga
     1381 cgggtatgaa tgaacatcct tatatgtcgg gttattttta tttgacggtg gatgacaaaa
     1441 accgccaagc attgatgcaa gcagttactc gcctattaac cgaaaagaag ttgaatattt
     1501 tggtaggaac cgttagtttg ctaggagaag gctgggattg tcctgcaatt aatctgttga
     1561 tcttggctaa tcaagcttcc acttttgtgc aaacacaaca actacgaggg cggggcttac
     1621 gcctaggtgc acaaaataaa gtaacgaaca tttggcattt agcgattgga ttaccgggag
     1681 ttcctctgtc tgaacagcct gatttgaaaa gaatgctacg gcgtttgggc aatatttcag
     1741 gtctgacctt tgaatcttca ccaaaaattg agagtggtgg cgatcgcttt gctttaccac
     1801 tggaatatac gaccaccacg atggaaactt atactcagtc gatgttctat ttttcttgtg
     1861 aacgaagtcg gttgaaagaa caatggagta ctgctttagc tgatggctgg cacctatcta
     1921 tgccaatcgt cgttaggaaa gtcgatcagg taaagcaaaa acaagaaact tcattgccga
     1981 tagtagtaaa aaatatagag cctctaagct tctggtcagc tatatttaaa ggtaacttgc
     2041 tggaatattt ccaaattcgt aggcgacaaa agctatggga aaaggaatgt gcccgcaaaa
     2101 aaagcttggc catatgcgtt caaacgacgt tgcagcgcct tggtgaagtt agtgatagaa
     2161 aagtaatcgt agcggctgat cagcaggaat ttagatgcta tcttgagaat gctggctatc
     2221 gagaaggaca gctttttact gaagctatga gggagctttt agcaccgctg gagaatccgc
     2281 ggtatttaat cgaagggaaa aagatagttc tgacggtacc agaacgcttt ggccgaaata
     2341 agcaaatcgc taaccttttt ctaaacgaat taaatcaatc gtcgctgccc aaatgtcagt
     2401 tggtgtatac tagaaatcct gaaggacgcc aaaagctagt gacttatcga atgaggcaag
     2461 tcttccagac ccgcgatgaa gtaattattc aaaaaaacat ttggacgtaa gttgatgaaa
     2521 ataaattttg ctagagtaga acaagtccga gaattaagtt gctacttaac tgataatgcc
     2581 aaggacagga gaatcttttc ttgacgactg aaagaaacta tttgacttat ataacggtgt
     2641 gtgatgaata tttgcaaggc attctcttaa aaagtactcg taatagtttt tttgaaaaca
     2701 aaacagtact ttattaatag gactgacaga gcgatataag aacatctgca gacagaggtg
     2761 aatgcttttc tcatgatcga tgaaacaatg gtacttcgga tattcttgct tttagtttga
     2821 accgttgtga ttttctttga ttcctaaaag tagttttata gaatgaaaat caagcatacg
     2881 gaatgctcct ttgattgtgt tgtggtgatt caatctaatg gagcttctat atgttttttt
     2941 atttgcaaaa agaaccgata gaatattttt atcagtctta gaaattatag agccaagaaa
     3001 tttcaagcac acataaaaac gcgtgtttta tgcattttat gagcaaaaat aactaaagac
     3061 cattacgtaa aacataggta aatcagcatt caatctcaaa ttatgtgatc aaatgttgat
     3121 attatttgat tataagtttt cttttgctat tatcagtctg caaataaaca gattatagag
     3181 tatgaatgta gatagttcaa tctttgttta cgacctcaca tgttttttta ctcaagagaa
     3241 agtaatataa agtgactata tttgacagaa ctttacataa aaaaagtagt tagttttctg
     3301 tagtaatcag aaatgtaaat aaattaaaaa aaagaaattt agcagttata tatttttaaa
     3361 atattttggg tgagtagtca atacaacatt ttgttcatca agcattaaat gaagatgcaa
     3421 ggatgttaag aagttagctt cttcagggtc ttgcatggtc aaggctttaa atagttgatg
     3481 atgttgttcc ataatttttt cccattttaa tccttccgtt tttactcgta accaacgaaa
     3541 tcgttccaag tgcgtattat tatcttccaa ccattgccat acttcttccc gttctgcaat
     3601 ttcaaaacaa gctttatgaa ataaattatc attatgaaaa aaactacgag cattcttttg
     3661 actaacagct ttttcttgtt ctgcaattat agtttctaaa acttgtttct tttgattatt
     3721 catcttggca caacactcta gcataatttg tcgttctaaa tgttctctga caaaacgagc
     3781 attttttgct aaatctaagt cgattaatga gatataattt ccactttgag gaattgtatc
     3841 aacaagtcct tgatgacgga gttgaatcaa tgcttcacgt attggagtac gaccgataga
     3901 aagcgtttct tccaattttt tttctgatac tttttttcca ggttctaagt cagcgtagat
     3961 aatttgctga cgaatagatt gataagcttg attttggaga ctagttttaa ttgtcatatt
     4021 taatccctct caaaatttca tttttgaatt tgatatttta gttctatgct cgttatataa
     4081 tatcaaattt caaagtaaca tactagctat taaaaaaaca aaaaatactc aaataagatc
     4141 aaagagaaat taagctttaa tttaagttag ttctaaaaaa tatatattgg tgtgctttta
     4201 ctaaaaaaag acacaataat tctgacatgg tagaaaaaag aatattttta gttttttctt
     4261 attgacagcg attacattcg attttatact acaattgtaa cggaaactaa catatcagta
     4321 tggaggggta aatatggttt tgattaaaga ctggcaagat tctatcgaag gattgaacaa
     4381 aatgcaagtt caactaccgt attttaatgt cgaagcggtc aaacaagaag gtcagaaaca
     4441 tccagtgtgg cttcattttg gtggaggaaa tctttatcga ggatttcatg cagaaattgc
     4501 tcaatgctta gcagatcaaa atgaattaga atccggagta gttgtgtgtg agacatttga
     4561 tgaagaagta gtagaaaaag cttatcatga ctacaacaat gatattttgg agattgttat
     4621 gggtgaagat ggtgaactca aaaaaagact tttagcagta acagctgata gtttatattg
     4681 caatccaaag aatataacgg actatcaaag agtggttaaa tattttgaag atcctacatt
     4741 acagtttaca acgtttacta ttactgaaaa aggatacagt ttaaaagatt ctaaaggaca
     4801 atttttttcg gtagtggaag cagatataaa atctggacct accaatcctc aacatacgat
     4861 gagtattgtg acatccttgt tgtatagcag gtttaatgct ggagagttac cgattgcgat
     4921 ggtttctact gataatttct cacagaatgg tcaacgtttt caagatagca tttttatgat
     4981 tgcagaagct tggcaaaaaa aaggatacgt ttctgaaaaa tttattgctt atttaaagga
     5041 taaagaaaaa gtgacttttc cttggagtat gattgataga attacgccaa atccttcgga
     5101 aagtgtcaaa atggatttat tgaaggcggg tttcgaagac ttagatataa ttcatactgc
     5161 gaaaaatacc aatatcgctg cttttaccaa tactgaaaaa gtccattatc tagtcattga
     5221 agatagtttt ccaaatggtc gacctaaact ggaagcggct ggtgtgatta tgacagatag
     5281 agaaacagtt gataaagcag atatcatgaa ggttacgaca tgtttaaatc cgctccatac
     5341 ggcattagca ataacaggtt gtttgttggg gtataaatca attcaggcag aaatgcaaga
     5401 tgatgatttg gttggcttaa ttaaagggat aggttatgta gaaggcttac cagtagttga
     5461 aagtccaggc gttattgatc caaaacaatt tattgatgaa gtaatcaata aacgtttgcc
     5521 taatccctat attccagata caccgcaacg aattgcaaca gatacttctc aaaaagtagc
     5581 gattcgctat ggtgaaacaa ttaaaaagta tgtcgaaaaa gacggaaatg cagaaaatct
     5641 aacatttatt cctatcgcta ttgctgcttg gttacgttat ttattggcag tcgatgatca
     5701 aggaaaaaat tttgtgccaa gtcctgatcc tttattggta gagttgcagg aaaaattctc
     5761 agtattgaaa ttaggtgaac aagatcctgc aaagattcat tgtgcggtac aggaaatctt
     5821 agaaaataag gaaatatttg gtttagattt atatcaagtt gagataggaa agaaaattga
     5881 aaaaatcttt tgcgaaatgc tagtaggtcc tcaagcagtt cgtaaaacaa tcaataaata
     5941 tataacagaa gttggaggaa agtaactatg gaaatgactt ttagatggta tggggcaaat
     6001 gaagaaaaaa tctcactaca agaaattcgt cagatcccag caatgaaagg aattgttgga
     6061 acattaatgg atataccagc cggagaggtc tggccgagag aacgaattaa agctttaaaa
     6121 gatgaagtcg aagccaatgg ccttaccttg aaagttattg agagtgttaa tgttcatgat
     6181 gatatcaaaa ttggtttgcc aacacgagat caatacatcg agaattataa acagacgatt
     6241 cgcaatttgg cagaatttgg gattgaggtt atctgttata attttatgcc agtctttgat
     6301 tgggtaaaat cagatttgga ttatcattta gatgatgggt cttctacgtt agcctatatt
     6361 tcaaaaaata ttcctactga tccagaggaa attattagaa aagtagaaaa ttctactaat
     6421 aattttgaat tgccgggttg ggaacctcat cggttatcac aaatcaagca attattagaa
     6481 gcctataaag aagttgatga agcgaaattg cgagagaact ttgcatattt tctcaaagca
     6541 attatcccga catgtgaaga agttgggatt aaaatggcag ttcatccaga tgatccacca
     6601 tattctattt tcggattacc tcgtattgtt aaaaatcgtg acgatttaga atggttgtgt
     6661 aatgtagtgg attctccttc taatggtatt acgttatgta ctggaagtat tgcagaacga
     6721 ccagataatg atgtctatga aattctatct gaattttgtc aacgggatcg gattcctttt
     6781 gcacatgttc gtaatatcaa atatttaagt gataatggac tcgattttta tgaagcgccg
     6841 cataaatcag aatatggttc tctagatatg tataagatat taaaaacaat gtacgataat
     6901 ggatttaatg gctatattcg tccagatcat ggtcggatga tttggggaga aaccggtaga
     6961 cctggttatg ggttatatga ccgagctcta ggtgcagttt atttaaatgg tatttgggaa
     7021 gcgcttgaaa agggggcgag taaataatta tataatggag tctatttaga agaaaaattt
     7081 tctcttctaa aaactaaggg aaggaaagga gtgcttaaat atgtttttag ataagaactt
     7141 tttattagag aatgaaatag ctaaaagact ttttcataaa tttgcagaac accaaccaat
     7201 catcgattat cattgtcatt tagacccagc atgtttgcta gaaaataagc aatacaagaa
     7261 tttgactcag gtatggttaa atgataaagg agctggagat cattataaat ggcgattgat
     7321 gcgagctaat gggatagaag aagagttgat cagtggtgca ggtaatgatt atgaaaaatt
     7381 tttagctttt gtaaaaacag tggagaaggc gccaggtaat cctatttttg aatggtctca
     7441 tttggaatta caacgttact ttgatattga tttgacaatt tctgagaaga atgcaccaga
     7501 aatttggcga cgtgccaacg aaaaaatcca aacggattcc tatcgcccaa aagaattgct
     7561 gaagatgatg gatgtgaaag ccatttgtac aacggatgat cctacagatg acttgcgata
     7621 tcatactgag ctttctaaac aggataattc tttcaaggta ttacctactt tccgtccaga
     7681 taaggtctgg agtatcaaat catcaggatt ttcgaactat attttaaaac ttagtcaagc
     7741 agcgggtata ccaatcaaaa actttaatgg gttgaagcag gcattgagcc aacgagtgga
     7801 ttattttcat caaattggct gtcgattagc agatcacggt ttgaatactt atttttatga
     7861 aatagcagat gaaaaaatac tcgacaatct gttaataaaa gctttagcag gaaaaacgga
     7921 tttccattta tcagaaattg cacaatttgt tacaggtatt caattacatt tgatgtctga
     7981 gtatacaaga tatggttgga cgatgcaaat gcatttaaat gctttaagaa ataattcaag
     8041 tatattaaag gaacaaattg gagtagatgt cggtggtgat tcgatggggg atcaagctag
     8101 tttgacacaa aatgttgtag cattgttcga cactgctgaa aagagtgggt gcttaccaaa
     8161 aacaatttta tattcattaa atccaaccga ttggcttcca ctagcaacag caatgcaaag
     8221 cttccaaggg ggtatgattc aaaaaatcca attgggatgt gcttggtggt tcaatgatac
     8281 agctgaagga atgcgtcaac agttgactac gatggcgcaa caaagtttgt tgtcgaattt
     8341 tacaggtatg ttgactgatt ctcgaagttt tctaagctat cctcgccatg agtatttccg
     8401 tcgagtatta tgtcaattac taagcgagtg ggttaaagca ggtcgaattc cagatgatga
     8461 aatgtatctt agtcagatag tggagaatat ttgttatcgt aatgcgtata attattttaa
     8521 tttttttgaa atcgaaagta gaaaataata caaaaaataa aagcttaaat ttatttattg
     8581 taagttagta caactagcta gagtatgtat ccataaaaga aggagataga tatgaaaaaa
     8641 gtagaaattt taagtaagtt agaagcggct ggagtcattg cggttgtacg tggaaaaaat
     8701 aaagatgagg ccattaaagc aataaatgca attactgcgg gtggaattaa agggattgaa
     8761 ctaactttta cagttccgaa tgctgaagaa ataatcaaag aaattgctaa aaaatatgct
     8821 gatcaaaaag atgtaataat tggagccggt acagttttag atgcgtttac tgctcgtatt
     8881 gcaattatgg ctggagcaga atatatcgta agtccatctt ttgatcgaca aacagcagag
     8941 atttgtaatt tatatcaaat tccttattta ccaggttgta tgacaattac tgagataaag
     9001 gaggcactta ccgctggagt agaaatcatt aaactatttc caggttctgc ttttggtcca
     9061 tctgttgtaa aaacgtatca agctccatta ccatatgtaa atataatgcc tactggcgga
     9121 gtaagtctag ataacttaga agagtggttt gaagcaggcg ttgttgcagt tggtgtaggt
     9181 gggaatttgt tggctcctgt tgaaaaaggt gattttgaag aagtaactcg aatagctagt
     9241 aaatatatag ataagtttta tgagataaag aattattagg agaaaaaata aataatacta
     9301 taaaatgtaa aaaataaata ctaactcttt atacttcaga gtatagtacg taagttggca
     9361 gttcaacaga atgaaaacgc ttaacaatag gaaggagcgc aaattatatg gaaagaaaat
     9421 ttggtctaag agacaaaata ggatatatgt ttggagattt tgggaatgat ttcactttca
     9481 tttttgctag ttctttttta atgatttttt atacaaaagt attggggata agcggagcta
     9541 ccgtaggaac attattttta gtagcaagat ttattgatgc agttacagac ataacaatgg
     9601 ggcgtattgt ggatggatca aaaccatcta aaactggcaa atttaaaccg tggattttac
     9661 ggatgagtgg tccagtcgct ttagcaagtt ttttaatgta tcaaacatca atggcatcag
     9721 cttctatgag tattaaagtt atatatatgt atgtaacata tatactatgg ggaagtattt
     9781 tttatacatc tattaatatt ccttacggtt caatggcatc agctatcact tctgttgcag
     9841 aagaaaggac agcgttatca acatttcgta gtataggagc taccttagca ggtatgttca
     9901 ttggcgtgtt aacaccaatt tttatttata caagagatag ttctggagct caaattgtac
     9961 gtggtggatc aacttttaca attgtggcag gagtattttc agtgtgtgca gttatttgtt
    10021 acgtaatatg ttataaatta actgtagaac gggttaaaag tgatgaatcg cagttaaaag
    10081 tttcattgtt gaaatcttta aaaggattag ctaaaaatag agcgttgctt tctataatag
    10141 ctgcagctat ttttttgttg cttgctcagc ttttaacaat gagtatgaac aattatgtat
    10201 ttccagatta ttataataat gcacagggaa tagttttaat gaactttatc aatcctattt
    10261 tgatgttggt acttgtttca cctgtaagtt taattttcag taaaaaatac ggtaaaaaag
    10321 aattggcgtc agtagggatg ttcttctctg ctattgtata tttactttta tttttgataa
    10381 agcctagtaa tatgtatgta ttcttagttc ttacttctgt tggatatatg gggttaggta
    10441 tttttaatac agtcatttgg gcgaatatta ctgatgtaat cgatgatcaa gaagtgaaat
    10501 ctggccaaag agaagatgga actatttatg cagtatattc ttttgcccga aaaattggac
    10561 aagctttagc tggcggtgtc ggaggttgga ctctttctat aatagggtat gattcgttag
    10621 caaaagtaca aagtggagaa gttttacaaa agctgtataa ctcttcaacg ttgattccag
    10681 cagtttgttt ctttgttgta ggattgattt tatttttcgt atatccatta tctaaagaaa
    10741 aagtattaga aaattataat ttgctaaatc aaaaacataa ctaggagaaa aaacatgaac
    10801 agttggatat tttgtattga ttcagatggt tgtgtcatgg atacaatgac ttataaacac
    10861 aatctctttt tcgggccaat tgctgcagag atttttgatg ttcatcatag ggaaaaattc
    10921 ctacaggatt ggaataatat taatttatat tcagagactc gtggaataaa cagattttta
    10981 ggtttagtta aaacattaga aaaaaatgat gtaccatcga ttaatcgatt aaagcactgg
    11041 gtatctgtaa caaaagaatt gtctaatcca tctttgaaga gggaaataga atactctcag
    11101 tcagaggatt tattaaaagc tttaaaatgg tcggaagaag tcaatcgaca gattagtcaa
    11161 gctaaaggat tagataaacc ctttttaagt gctagagata ctataaaagc tctgaaaaaa
    11221 tatgggaaga taattattgt aagctcagct aataaggaag ctattcaaga agaatgggaa
    11281 agacatgaat tattatcctt agttgatgaa ctgtgttgtc aagataaagg aaaaaaagaa
    11341 gacattattc gaagtgtaat tgaaaatggt tgtgatttgg ataagatatt aatgattggt
    11401 gattctcctg gtgatctaga agctgcaaat aagaataaag ttttctttta tcctattttg
    11461 gttaataaag aaaaagaatc atgggaaaac ttaagaactg atattttatt taaatgttta
    11521 tcgggagagt atatagatat tgaacaaaaa tatatagata ctttttggaa aaatcttact
    11581 aataagtaga ggagaaagaa aatgactata aaagtagact tacgaaaaaa accatatcat
    11641 ttagatgaag atgctatttc ttggattgaa gagacgattg atgctatgac tttagaagaa
    11701 aaaataggtc aattatttgt taatatggga tctagtcgtg ataaaaatta cttaagtagt
    11761 atggtcaatc agtaccatat tggagctgta cgatataatc caggaactgc tgatgaggta
    11821 tatgatcaaa actatatcct acaaacagaa agcaaaattc ctttattaat tgctgctaat
    11881 acagaagcag gtggtaacgg agcttgtaca gatggtaccg aagttggtgt agaagtaaaa
    11941 attggtgcaa caaatgatgc gaaatatgct tatgaattag ggagagtttc tggaattgaa
    12001 gcatcggcta ttggatgtaa ctggagtttt gctcctattg tagatattaa tagaaattgg
    12061 agaaatccta tcatttctag tcgatctttt ggacaaagcc ctgataaagt tttagaactt
    12121 agtttagctt atatgaaagg tattcaagag agtggaattt tgcctgcagc taaacatttc
    12181 cctggtgacg gtattgacga acgagatcaa catctatctt ttagtattaa tagtttaagt
    12241 acagaagaat gggataattc ttttggaaaa gtatactcag gcttaataga agctggatta
    12301 ccttcaatta tggcaggtca tatccatatg ccagcttatt caaaacattt taatcttgat
    12361 ctgaaagatg aagatttact tcctgcaact ttatcaaaag aattaatcac tgacttactt
    12421 agaggaaaac tcgggtttaa tggattagta gttactgatg ctagtcatat gttaggaatg
    12481 actggggcaa tgaaacggtc agaattattg cccacttcta ttgcggctgg atgtgatcta
    12541 ttcctatttt ttaatgatcc agatgaagac tttaattata tgatggaagg ttattacaaa
    12601 ggaattattt cagaagagcg tttacgtgat gcattaagaa gaattttagg cttaaaggct
    12661 aaactaggtt tgcataaaaa agaaaagaat aaaattttgt taccgaaaca agaagctatg
    12721 gaaaaaattg gtttaattga aaataaatca atttttaaag aggtagctga tcaagcaatt
    12781 acactagtta aagataaaca agatatttgg cctattgtac cagaaaaata taaaaggatt
    12841 ttactggtag atgtacatgg ggtaaaaggt gggtttggtg aagtagttgc tggaaataga
    12901 cctcaggcca ttgatttatt gaaacagatt ttgtcagcaa aaggatttga agttgaaaaa
    12961 tacatttcac ctgaagaaga attgaaagga ttatcgcaag aagaaaaaat taaaaatata
    13021 gctggaatat atgctgctaa gagaccaatc aaagatttaa ctgaaaaata tgacttgatt
    13081 attaatatag ctaatgttaa cccaggtaca gttcaaagaa ttgtttggcc agcaagtaaa
    13141 ggtactccgg atattccatt ttatattcat gaaattccaa caatttttgt atctgtacag
    13201 tgtccgtttc atttagctga tgtgcctcaa gtaaaaactt atatcaatac ttatgatgga
    13261 aaacaagaaa ctttagaaat attagtggac aaactaatgg gcctttctga atttaaggga
    13321 ataagtccaa tagatgctta ttgtgggtta aaggatacat ttatttaata tttaaattat
    13381 ataggagtga tttacatgtt atatcccatt atgacagaga cgaggcaagt aattgattta
    13441 agcggtcttt ggagatttaa gttagataca aatgatgact tacaagaaga acttgcttta
    13501 gaaccaatgg ttgaaaaaga gagttatcca atttcagttc cttcttctta taatgattta
    13561 tttgaaacag agaacattag aaatcatgtt ggatgggtgt ggtatgaaaa agaagtgatt
    13621 cttcctaaaa aattattaga agaaagatta gttcttcgtt ttggatctgc aacacatgaa
    13681 gcaaaagtat ttttgaatgg aaaattgatt aatcaacaca aaggagggtt tacgccattt
    13741 gaatcagaaa ttaatcagtt tataaaagat ggaagtaaca gaattacagt ggctgtaaac
    13801 aatgttattg acgatagcac tttacctgta gggattttga aagaaacgat acaggaagat
    13861 ggagacataa aaaaagaaaa tttggttaat tttgactttt tcaattatgc aggcattaat
    13921 agacccgtta aattgtatac aactccgaaa gactatataa aggatgtcac tattaataca
    13981 aaaataattg attcagatgc aaatgtagaa tttaatatcg atacagaatg gaccggagaa
    14041 attctaatag caatctatga cgaagaagct caacttatag cagaagctaa ggggaaagaa
    14101 ggagtgattc aacttaagaa cccaacactt tgggagcctc taaatgctta cttatatagt
    14161 atgaaagtta gtttaattaa cgaaaataaa gtgattgata cgtacacaga agaatttggc
    14221 attcgtagta ttcaggtttc gaatgggaag tttttgatta ataataaacc tttttacttt
    14281 aagggatttg ggaagcatga ggacacgtat attaatggtc gtggtttgaa tgaagcagta
    14341 aatatcaaag attttaattt aatgaaatgg atcggtgcta attcatttag gacttcacat
    14401 tatccttatt cagaagaaat tatgcgctta gcagatcgag aaggtattgt agttattgat
    14461 gaagtacctg cagtaggatt acatttgaat ttcatggcta ctttattaga tgacaacgta
    14521 gaaaaacatg atacttggaa agaaatacgg acagaaaatg ctcacaaaat ggcgattaag
    14581 gaattgatag aacgcgacaa gaaccatccg agtgtcgtaa tgtggtctat tgcaaatgaa
    14641 ccagattcgg attctgaagg cgctaaagaa tattttgaac ctttagttga attgactaaa
    14701 gaacttgacc ctcagaagag acctgtaaca atcgttactt acttaaagtc aacaccagat
    14761 atttgtaaag taggagatat tgtcgatgtt ttatgtttaa atcgctatta tggttggtat
    14821 gtttcaggtg gagacctaaa acatgcaaaa tatctgttga aaaaagagct agatggatgg
    14881 atggaaagat gtcccgatac gccaataatg tttacagaat atggagcaga tacagtagct
    14941 ggacttcatg atacagtacc aacaatgttt acagaagagt atcaagtggg ttattacgaa
    15001 gcaaatcacg aagtaataga taaatgtgac aattttgttg gtgaacaagt gtggaatttt
    15061 gcagattttg aaacaagtca agggatttta agagtgcaag gaaataagaa aggaatcttt
    15121 actagagata gaaagcctaa aatgattgct cactatctaa agaaacgatg gtcatcaata
    15181 ccaaatttca gttataaaaa ataatttacc ttttataagg aatataaaaa aggagtggct
    15241 gcttaattgc caactccttt tttttataaa aagattttct gtttaaagaa ggatattttt
    15301 ttctagtttt ttcgaaaaaa aggcgctttc tgattgttgt attagtggaa aggggaaaaa
    15361 aggagcatat ttatcatgca acaaaattac aatgagaaat ctatacaaca tcaatttgat
    15421 gcctactgta aaaaagtatt acgaaacgaa actaagtcta ttcgaaaacg aaacattaac
    15481 ataacagata atgaggaacc attgacgaat tcaaatgaag gtaagtactc ccaaactcat
    15541 tttgatgaac aagatgtcta tttcttattt ggaatggaaa tattaatttc agatcaaaag
    15601 ttatcggcag ccattgatca tttatcggaa ataagaagaa aggttatcct actttattat
    15661 tttgcaggtt ttaatgatac tgaaatcggt aaaatcctca atatgagtac aagtggtatc
    15721 tggtatcaac gaaagaaggc agttgaacaa ttaaaaatgg ggtatgactt atggtagaca
    15781 accccaaaaa acggcagtat ccattagttc ctctatccat tatccaatct gctctagaag
    15841 aagatccaat ttcaatcaag atgatactca aacattatca tagttatata gctaagttat
    15901 gtttgacaaa tggatttaat gaggcggggc aatttattac ctatgtagat gagtatatgc
    15961 taagacagct agaaattaaa ttgatagaag cgatcttaaa atttaaaatt aactaactaa
    16021 aaagtcccct ttccacttat tctgtttaaa agaatcttct aatagttaaa ggattagtag
    16081 attcttttaa acagaaaacc tattgatcat tgaaatttaa atacattcta tcagtacgtt
    16141 aacacttcaa ggagcatatt ttagttgcgc catgatttct tactgtaaag aaggagcgat
    16201 aaacattcta aaataatttt tatagtcaat aaaaagcgat gatttgaagt tgggataatg
    16261 atacttccgt aaggctcgcc gtaaacttgg agagaggtga aattcctatg tgcttttgac
    16321 aaaagcagct gattaagaat tatttccata tgttgatttc aacatttttt ctattatttt
    16381 taagaccaaa ttcagtgagg caaatagcat catgtaaatt tagtttttct attgaacgtt
    16441 ctccactttt tagttttttt atagtactca aatttaatcc tgtatgttta gagatctcat
    16501 ttgcagtatg gttttgtaaa gtttctcgga ttattgttat attagcaatt tcattacgca
    16561 taattatgtt cctttcttat caaaaatatc aatgactaca gtgggctttt agtgaaaaaa
    16621 aggggtacta cagtctcttt tgaaaacaaa aaaatacagt atatacgttt tctaaatgct
    16681 aagcaccaac gtttacactg tatttaaggg tttggcaccc aatgggctgt gaggggctcg
    16741 aacccgcgac ccgatgatta agagtcatct gctctaccaa ctgagctaac agcccaaatt
    16801 aatactttgt tattctaaca cctaaaataa aaaagtgcaa tacttattat cagaattgag
    16861 agtgaatttt atggattttc aagattatga aaaaatattt gaaagttatc catcgatatt
    16921 gaaaattact gatttaacta aaatgttaaa tattggaaaa aataaagctt ataatttgct
    16981 gaaagataga tcaatagaaa atataaaaat tggagcaagg tacaaaatac ctaaaaaagc
    17041 tgtcattgag tatatattga aaatacaaga tgaaggtaaa aatcatgcta taataaaata
    17101 gtcaaaattt ttgccgatat ttcagtttaa gaggagagat tgaattgaaa ttaagagcaa
    17161 acataattga aaaaagagga acattacaag tagttgtatc ttataaagac tctaacggaa
    17221 aaattagaca aaagtggaaa gatacaaaat taaaaaagaa aggaaataaa aaacgagcag
    17281 aaaaaattag agatgaattt ttactagaat tggaaagtca attagctatg gaatgtgaat
    17341 gtgctaatcc aaatttattg ttttatgact atttgcttaa ttatttggaa gtatcaaaaa
    17401 aacaagtagc atttaacaca tatgttgggt ataagcatta cgtccataat cgaatttaca
    17461 agttttttta tcctaaaaaa attaaactaa ataggttaaa gccttatcat ttacaagagt
    17521 tttatcaata tatgttaaat gataactgta cagcaaatac ggttattcat tatcacgcat
    17581 ttatacgcaa agctcttcaa gaggctgtta ttacagaatt gataagtaca aatgtagcag
    17641 ataaagtaag aaagccaaaa aagaaacaat ttgtttctca agtttataat caacaagaga
    17701 tcttgaaatt acttgacatt ttgtcagaag aaaaactcta tttggtagtt cttttaacag
    17761 cattttatgg attgcgaaga agtgaagttt taggattaaa atggtctgct attgattttt
    17821 taaacaaaaa aatcatgata aaccatgtaa tcatagaaaa tccagagaat atgtcacagt
    17881 taataaagaa agatcaaaca aagaactctt ccagctatcg tacattacct ttggttgaaa
    17941 cgattgaacg agcattagta aatcaagcta aatggcaaca agataatcgt attcttttag
    18001 gagatgatta tattttagaa gataatgagt acgtatttac tatggaagat ggacggttga
    18061 tgaagcctca atatgtaacg catcgtttga gtaagttgat taagaaaaat ggattgaaaa
    18121 aaattcgctt tcatgacttg cgtcattcta atgcttcaat aatgttagat agtggccaga
    18181 atatgaaatc cattcaagaa tggctgggac atgcatcgta ttcgacaact gctaatttat
    18241 acacgcattt aaccgcagga gtaaaagatc gtgcagcaga agcattagaa aacgcatttg
    18301 ggtttaaaga ataaaaaaac aaaaaacaat atctttgggg tatcgcttct tgtttttaga
    18361 atttttttgt tggcaatttg ttggcagtat gttggcaatg aaaaaattca caaactaaaa
    18421 acctatttat aaagctttat aaaacttttt tcacaaatag agcatttggt taagagtcag
    18481 ctgctctacc aactgagcta acggcccgta caaaaagtac ttaataatag tagcactaag
    18541 aattttaaaa tgcaagacta catttaaaga aaatcagaaa aatctttact tccttatagg
    18601 aaatcattca tagattattc acatttgctc gctattatat aagcatacca acaaacaaag
    18661 ttcttgcaag agaacaaacc ctaacacttt tttcattttt tactcctttc ccgccgtctc
    18721 cccggcgggt ctcctgttct taacgataaa agacaagaag gcttagcctt cttgtttttt
    18781 gttttcatgg aatcccgcta atcaaatcac ctagttaaag caactagatt gaatgtttat
    18841 cataagtctg atatcatgtt tatgaaatga aaaaaggagc ggttatgatg gaaaaaatgc
    18901 gtcagcaatt aatggagtgg gggaaggaac tagaaacatt ccgtctgcct cactgggaag
    18961 aactccctga cttagaactt tatatggacc aagtcagaac gttagttgat cgttatcttt
    19021 cacctgtgat acaaggagaa aagcatccat tgcttacctc ttctatggta aacaattacg
    19081 ttaaactagg tttgatacca gcaccagtta aaaaaagata taacaaagaa cacgtagcat
    19141 ttttacttgc aatcactacg ttgaaacaag tattgactat tcctgaaatc aaagaaggga
    19201 tattgttcca aggaaaaaca gtaggaattc gtgaagccta taatctattt tgcgatgaac
    19261 aagaagcagc tgtgtggatg gtcagccaat tagcacaagg taaatctcat ccgcaaaaat
    19321 tcgaccaggc aacccctgta gaatatattg ctgtaagagc ggcaacttta tcgtttgcaa
    19381 tgaaacttct tgcggaaaaa acgattgttc tagaaaccga atatttaaaa gaggagaaaa
    19441 caaatgaaaa acaataaaat cgcaatttta gtagattcag gtacggatgt tccagaagaa
    19501 ttgataaaaa aatacgagat gtatatgatc ccattgaaaa ttatttataa agatcaggta
    19561 tatacagata aaattgacat tacccctgaa gagatctatc aacgtttgcc agaggaaatc
    19621 cctggtacat ctttgccgga tggtgagtcg atcacagaaa tctttgagca aatcaaaaaa
    19681 gacggttatg aaaaggtact tgcggtaaca atttccagtg gattgagcgg gacatataat
    19741 gtggttcgtt taattgcaca acagcaagaa gaattggaag catatgtact agatacgaaa
    19801 aatattggta ttggtgcagg tttttctgcg atccaagcag caaaatggct ggaagaagga
    19861 gtagaatggg atcaactgat cagtaatctg aatgagttag tcgaaagaac aaaagttttc
    19921 tttaatgttg cgacattgga atatcttcaa aaaggtggac ggatcggttt agttgcttct
    19981 attgtaggaa ccgctttaaa gcttaaccca atcatctcat gtaatgaaga agggatttat
    20041 tacacagtag gaaaagctag gggacgtaaa aaaagtttgg ataaaacggt tgcttacgtc
    20101 aaagaaaaaa tcggtaatgc aaaggtcttc aacttagctg ttgcccatgg ggatgcgaaa
    20161 gaagaagcaa ttgaaatgga agcgagactt aaaagagagt tcccacaagc aaaccaaatc
    20221 tactttggcc agatttctcc ggcattagta gtccacactg gtccgggatt attgggtgtc
    20281 ggtgtacaag ttttagaagg ataaatcaaa aaaaggaaac actgaggtga aaatcagtgt
    20341 tttccttttt tattgaagtt agaacaaaaa ataataaagt attttgcact ttcattttaa
    20401 ttttctcttt tgttttatag gcaaagatga aacaaaagca gaaatacggt ataatgagaa
    20461 ggtatgacaa taatatataa gaggtggctc tttatgaaaa aagttttagt tgtcgatgat
    20521 gagaagccga tttcggatat cgtcaaattc aatttagcca aagaaggcta tgacgtttat
    20581 actgcatatg atggggaaga agccttggaa aaagttgctg aggtagaacc tgatttgatt
    20641 ttacttgatt tgatgctacc taaaatggac ggcttagaag ttgctcgtga ggtacgcaaa
    20701 acttatgata tgccgatcat tatggtaaca gccaaagatt ctgaaattga taaagtccta
    20761 ggcttagagt taggcgccga tgattacgtg accaagccat tttccaaccg agaattagtt
    20821 gcacgtgtca aagcaaatct tcgtcgcggt gccacggctg cgaaggaacc agaagaggca
    20881 gcacctgcgg aattgacgat tggtgatttg acgatccatc cagaagctta catggtgacg
    20941 aaacgaggag aaacaatcga gttaacacat cgtgaattcg aactactctt ttacttggcg
    21001 aagcaccttg gacaagtgat gacaagagaa catttattac aaacggtttg gggttatgat
    21061 tattttggag atgtacgaac agtggatgta actgtacgtc gtttgcgtga aaagatcgaa
    21121 gataatccaa gccatccaaa ttatttagtg acacgtcgtg gtgtcggtta ctatttgcgt
    21181 aacccagaac aggagtaatt ggatgaaaag aaaagtccgc ttttttcgat cagtcaattt
    21241 taagatcgct attaccttta ttctgatttt attgatctcg atcgaaatta ttggtgccta
    21301 ctttatacgt ggtttagaac gttccacgat caatacattt attaaagata tgaatcaaac
    21361 ggtggaatca ttagcaacaa caatcagccc ggaattgaat cgaaaagata atgcagatga
    21421 tgaagaggta aatgcgaata tcaaaagatt tattgaaaac agtgcgacta gcgatatcat
    21481 cgaaatacgg gtcgttgacg aaaaagggat catccgagga acaaccgatg tcaatgaaca
    21541 aagcgctgtg ggaaagaaaa atgattatgt tgatatcaat gatttcacga ctaaacgtta
    21601 tgttgcttta gataacgacg ataaacgggt gaatatcaat gttcaaccga tcctgtcccc
    21661 gaccggcgat gccgttatcg gggctcttta cgtcaaaagt aatatcgaac aaaaatattc
    21721 ggaaatcaat aacacagcag tcattttctt tactgcctca ctaattgcgg catttatttc
    21781 aatgatcgta tctgttttag ttgctcgttc gattacacag ccgatcggtg aaatgagaga
    21841 acaagccatt cgaatcgcta gaggagacta cagtcggaaa gtcaaagtgc atggacaaga
    21901 tgaacttgga cagctggctg ataccttcaa tcagttagct gaacgtattg aagaagctca
    21961 agatacaatg gaagctgaac gcaatcgttt ggatagcgtg ctatcccata tgacagacgg
    22021 cgtcattgcg acagatcgca ggggtaaagt cattacgatc aatgatatgg ctgtttcctt
    22081 gctagatatc aaaaacgagg aagcaatcgg acaatcgatt ttgacacttt tagatatcga
    22141 agaagaatat acgttgcgga aattattaga agatccaaac gaaatggtac ttgaccgttc
    22201 aaccagtaca ttggaaagtg atcagatgat cttgcgagta gacttttcta tgatccgtcg
    22261 cgaatcaggt tttatcagcg gattagtagc tgtcttgcat gatgtgacag aacaagaaaa
    22321 aacagaacgg gaacgtcgtg aattcgtctc taatgtttcc catgaacttc gtacaccgtt
    22381 gacaagtatg cgtagttata tcgaagcatt gagcgaaggt gcgtggaaag acgaggaagt
    22441 agcaccaaat tttttgaagg taacgctaga tgaaacagac cgaatgattc gaatgatcaa
    22501 tgacctctta aatctatctc gcatggatac aggtaatacc cagcttcaat tagaatatgt
    22561 caatttcaac gagttagtga attttgttct tgaccggttt gatatgatgg tgggaaatca
    22621 ggaaaaaaat tataagatcc gtcgtgaatt tacgcaaaga gatctttggg tcgaagtaga
    22681 tacagataaa atcatccaag tagtcgataa tatcatgaat aacgcgatca agtattcacc
    22741 agatggggga acgattactt gtcgcttatt agaaacgcat aataacgtga ttttaagtat
    22801 caccgatcaa ggattgggga ttcctaaaaa agatctgaac cgagtgttcg agcggttcta
    22861 tcgagtagat aaagcacgtg caagagcaca aggtgggaca ggacttggat tagctatttc
    22921 tagagaagtg attaaggcgc atcgaggcgc tatttgggca gagagcaagg aaggaaaagg
    22981 gtcgactttt tatatctcgc tgccatatga accttatgag gaggaatggt gggaatgaga
    23041 atctctgaaa aagtcgttcg tataggactc attttgatgg ttgcgttaag catatacttc
    23101 tcttatgcaa tctggctgag cccagcagga aaaagttcaa tcaacgtaga tgagacaaac
    23161 actcaaatga tcgagtctca aagcaatcga aaaacttccg aagtctttct tccgcttcat
    23221 gttacatggc accattcggg aatgatccaa gaaaccaaca gtgaaaatct cgtgtaccga
    23281 ttgcaattga tcatcgataa agcaagattt ggaaagcttt ctgaagtagt ttcaggagac
    23341 aaatcacagt ttgaacaggt gaaaaatctg aactctggtt ttgagttggc ctataatgcg
    23401 ccttttttgc taagagaata caaggaagca tttgatttag atttagatgt gtcgaacttg
    23461 gcaaaagaaa gcgaaaatat gtattttact agaatacaat tcgatcaagg aaataaaaaa
    23521 atccgatttt tgaattattc gaaccatctc gtatatgaag caaatatttc gttgaattgg
    23581 acagatatca ataaagaatt aacagcaaaa gacacaaaat ggacagaaat gacaacagaa
    23641 ccatcgacga tcgacacgca atatgctacg aaacattcga tcaaactgaa gaagtatagt
    23701 tatatcctgt cacagcaacc ctatacactt tttagaaatg cttttttcca gaaaccagat
    23761 agcgtgaaaa ataatgatga aagcaatgaa ttgatttttt acggtgggaa tgaaacaatg
    23821 tctatccatt cggaaacgca agtagttgat tttcgtggag agctgccaga gaaaaaagaa
    23881 aatgctagtg tattttctga aagtttcccc tatattagca aattaggcag ttctttaggg
    23941 aacctcagat attttgaccg cagtgacaat acgatcgatt atcggatatt cgtggaaggc
    24001 ttcccggttt ttgggacaga cagcagagga cagattggtg tggaagttgg cggtgaaacg
    24061 aatggttctg tgcaagtgaa tatccaaacc agtttgaata cgattcaagt gccgatacct
    24121 tcagacgaag aagtagaact tgcaagtacg aatgaaatca tccagcagtt aaaagctaag
    24181 ggagcagata gcaacaagat tggttcagtg atcgttggct atacatggca aaatgtcaaa
    24241 gaaacgaatc gtgtcgttga tctcttacct gaatggtatg tgaaatataa agataactgg
    24301 tattcagcta acgaccttct ggaaaaatta cctggattgg aggcgaatta attggatttc
    24361 aaaaaaatcg aatggatatt tttcgtcgct tttttagggt tgaatgtgtt tttattcagc
    24421 agttatcatg acgcggtata ccaagaaaac aatgtcattc gctctaatca gactgaatca
    24481 atccaacaac gtctggctag cgacgatatc acttacacgg ggaagatttc gtctgagaac
    24541 aagcaaggct attatcttag cgcggagcag accaatctta gcacttcatt agcgaattat
    24601 cgcaaggctg gtaaaagcgg cttgctggca agtggtgtgt ttttagacgg tgatgttttg
    24661 acgaaatctt tatccagttc agatgctgaa aagaacgtga tcgatccaga taaaattagt
    24721 agttccttgg aagatttttt atctaacaaa gacaatattt tattcggtaa tgattatgtg
    24781 tacacgaaaa acttttctca tttagaagaa gaaccactag aaatccaagc aagccaagag
    24841 tatgaaggca tcccattcaa tgatgataca gcaaaaatcg ttctttcttt agaaaaaaac
    24901 gataatgatt atagcatcac gaaatacatg cagacgcatt tatctgatat cgagcagctg
    24961 cgcgaaaaga cggaactgta tacggaagag gaagcaatca atacattgta catcaataac
    25021 aaaataccaa gaggatcaaa aattttatgg cgtcagctag cttattcctg tattctgaaa
    25081 gtccgggaaa aaaatgtgta tgttcctgtt tggcatgtag cgatcgaaac aagcgataaa
    25141 gtggtccaaa ttgagagcgt caacgctttc agcaatacga tcgtgacaaa caatactgta
    25201 ccaaaagtgg aagatcaata aaaaagcgag tataatgaaa aaagtttaca aagtaagaag
    25261 ggaagtagga gtcaaatggc tgaagaaaaa ccagctttta aaatcagtat cctagcaagt
    25321 ggaagttcgg gtaattcact ttatatcgaa agtgaaaaaa aacggctttt ggtcgatgca
    25381 ggacttagcg gtaaaaaaat cacttccttg atggctcaaa ttgatcggac accggaccaa
    25441 ttagatggaa tacttgtgac gcatgaacat cgagatcata tacatggcgt gggtgtcttg
    25501 gctcgtaagt atcatttgga tgtttttgcc aatcagcaga cttgggaagc gatggaacca
    25561 ctgattggaa atgtaccagt ggaacaaaaa catctatttg agatggggaa agtcagaaca
    25621 ttcggcgata tcgatattga gagtttcggc gtctcccatg acgcagcggc tccgcaattt
    25681 tatcgtttcc ataaagaaaa tcggtcattc gtcatgttga cggacaccgg atattgcagt
    25741 gattatatgc gaggtgtcat tgaaaatgcc gatggttatt tgatggaaag taatcatgat
    25801 cttgagatgt tacgcatggg accttatccg tggaatttaa aacagcgtat tttaggagat
    25861 agaggacatc tttccaacga agacggtgca cttgtcatga ctgatgtgat cggtaatcgg
    25921 accaaacgaa tctatcttgg ccatcttagc aaggaaaaca atatgaaaga acttgctcat
    25981 ttaacaatgg aaaatgtttt gaaagaaaaa gattttggcg ttggacatga ttttgaaatc
    26041 tatgatacag atccagatga agcaactgag ctattttcga tttaaacagt tcaatcaata
    26101 aaaatataaa aagcagaaga taaaatcaat cagggttggt tttatcttct atttttattt
    26161 ttttagcata ttaaaaaaat aaccgtatta tcgactgact acactgatgt gctgcttttc
    26221 atgtttctca tccagtatct catctactaa tgcaatcgcg taatctgcca tgctgatcgt
    26281 actgtcgcta gcagcgtttc tttgtaaata atcatcactt aacaaataag tacctgtacg
    26341 tttgccatgt ggatcaaata aagaagctgg actgatataa gtccaatgta gatttttaga
    26401 ttgtttcaat tttaacaatc cttcgtacat attgaaagct gttggataga agggtgcatt
    26461 tttaggtact tggtcaatca tacgagtcgt cttttctgta tctaagaata acgaagaggc
    26521 acccccaacg acaattaagc gtgtatcggt tccttcaagc atctcaatca aatgggacaa
    26581 agaagtttga tgcatttctt cttttccttt gggagcgttg aaagcatcaa cgacaacatc
    26641 aaaagatgca agatcagtag ttgtcaactc aaataaatcc ttttgtataa acggaacatc
    26701 tgatggcaat ttttctggat gacgcacgat tgccgtcacg tctaaatctc tggacaatgc
    26761 ttcgtctaaa ataaatgaac ctgcatggcc agtagctcca atgagtgcaa ttttcataaa
    26821 aaacctcctt ttttattcct gtccattgta agcagttgct gaatattcgt caaagaatcc
    26881 gatttttgaa aagctcagtg cgcattcctt tcatggttcg tgatcattgg gtatagttaa
    26941 attaagaaaa ctgattgata aaaatagaag ttgaaggaga taagaaatga aagagtggtt
    27001 agatcgtgcc tttttaccaa tgataacatc tgttgtgaat cttagaccct taactgcttt
    27061 gcgtaatgga atgacttaca cgatcccaat aattattgtt ggctcgctgt ttttcttatt
    27121 tgctcatttt cctgtggatt acatcgctca gtggatagac aaaacaggat tttctatgta
    27181 ttttacacaa gtataccagg ctacatttga tgtgatagcc ttttggatcg tttttgccat
    27241 tgcatacgct tatgcaaaag aagaagacat cgatcgctta ccagtcggca taacagcctt
    27301 gtctgcttat ctactgctta ttcagccact aaatactcag atagatactg tctggacagg
    27361 tgctgaaggg atactaggag cagttacaac tgggctcatc atcggatggg gctatgcacg
    27421 tttagtaaga cttgcgaaag ggtggaagca aagagagaag atacctgaaa atgtttggca
    27481 gtcttttact tcattgattc caatcattgt tatagtcact tttacgctta tcggttcagc
    27541 ggtttttcaa catttgttca gaatgactgt tgttcaagga gtatggacat atgtccagct
    27601 tcccattcaa tcgttcatag acacgttgtt tggtgtgctt ttattaggtt tttttgttcc
    27661 gtttttatgg ttatttggtc ttcatggatc tgcgatggtc aatgggttgg ttagtcccgt
    27721 cttgcaagcg aattcattag caaatgcaga aatcttagcc tcaggacaag agttgacggt
    27781 ggcaaatggc ggacatatcg ttactcagca atttctcgat cagttcatga cggttacggg
    27841 tgctggattg acgttagggg ctgtattttt tatgacggtt tttgcgaaat cgaagaaata
    27901 cagagaatta ggaaatcttg ctcttttacc agcctttttc aacatcaatg aatcaattat
    27961 tttttccaca ccgatcgtga tgaacccgat gatggctgtt ccttttattt tcgcaccggt
    28021 cgtttcagga ttgattacct attccgcttt atattttgga ttcgttcctt tatttacagc
    28081 agtccaagtc ccttggacaa caccgccgat cctctcgggt ttcttagtag gcggagtgcc
    28141 tgcagcgatt ttacaaggta tcgtgttgtc aattagtttt ttcatttatt tcccattttt
    28201 gaaaaaaata gattctgcaa actgcaaaaa ggaaagacag acaaaaaatg atggagcagc
    28261 agaaaagatg aacgactaaa aaacaaaaat actcctgtcg ctttagattg ggaaaatcac
    28321 caatctagga tgacaggagt atttatagtc atgtttatac tagccctttt tggacagcta
    28381 attgaacaag aacaactaat gtgttcattc ctacgtgtgt gatcatcgat gtccagatcc
    28441 gaccagttgc tttgtactct aggctgaaga agaaacctag gaaaaagtag attagcagat
    28501 gaccatcatt atgagcaaac gcaaacagca aagagctgat cactgctccg acccaaaaat
    28561 ttgaatatcg actgatgatc cccagtatac tgcggcggaa tacgaactct tccatgatgg
    28621 gtccagcaac agttgtcgcc aaaataaaag cgggtgcttc catgatcatc tggatgatat
    28681 tttgtgtatt ctcggaaggg gttgctttgc caaacaatac agattctaac atgacagcga
    28741 tgctttgcag aatcaatgcg acaaagattc caaccaatcc ataaataaca atctttggag
    28801 cagaagcttt ttttaccgct gattcgatcg ctaagggttc acgttgctta aaataaaaaa
    28861 tagctaggac aacggcgcct agtatataca tgactgcagt tgctgtgatc aattcagacg
    28921 tagattttac aagtcctatc gaagctagga tcaagggtga gataaaaatt agaccatagc
    28981 aaaaaatgct gaggaagcta tattttttta aagacatgta attcctcctt ttaagggata
    29041 acaagcatgc aaggcaagct tatatcgatt gatttcaatt atatcataat ttcaataagc
    29101 agctgtcaga ataaaatttc ttttctttga tttaaaagct tgcaaaaaag agggagaatg
    29161 agtattatta aaaatgtagg ttagcactcg aaggttaaga gtgctaacag attaaaaaaa
    29221 gagttatcgg agggatttat cgtgttaaaa ccattaggtg atcgcgtcat tatcgaagtc
    29281 gcaaaagaag aagaaaaaac tgtcggagga atcgttttag cttctgctgc taaagagaaa
    29341 ccccaaactg ggacagttgt tgcagtcggt gaaggccgtt tgttagaaaa cggtgaaaaa
    29401 gttcctgccg ctgtaaaagc aggcgaccaa gttatgttcg aaaaatacgc cggtacagaa
    29461 gtaaaatacg aaggaaaaga atatctgatc gtcgcaggaa aagatatcat ggcgattgtt
    29521 gagtaaattt ggaagataaa aaaataatca aaaataatca atgaggtgaa tcaatcatgg
    29581 caaaagaatt gaaatttgca gaagacgcac gcgcagctat gttacgcggg gtagataaat
    29641 tagcagatac agtgaaagta acattaggac ctaaaggccg aaatgtcgta ctagaaaaat
    29701 cttacggctc tccattgatc acaaatgatg gtgtgacgat cgcaaaagaa atcgaattag
    29761 aagatcattt tgaaaacatg ggtgcaaaac ttgtatcaga agtagcatca aaaacaaatg
    29821 atattgcagg agacggtaca acgacagcaa ctgttctgac acaagcaatc gttcgcgaag
    29881 gtttaaaaaa cgtaactgca ggtgctaatc ctctaggcat tcgtcgcggg atcgaattgg
    29941 caacaaaagc agcagtcgaa gaattgcata atatctcaac tgttgttgat tcaaaagaag
    30001 ccattgcaca agtagcagct gtttcttcgg gttctgataa agttggacat ttgattgctg
    30061 atgcaatgga aaaagttgga aacgacggtg tcatcacaat cgaagaatca aaagggatcg
    30121 aaacagaact agatgttgta gaaggtatgc agtttgatcg tggttactta tcacaatata
    30181 tggtaacaga taacgacaaa atggaagctg ttctagaaaa cccatatatc ttgatcacag
    30241 acaaaaaaat ctctaacatc caagatattc tgccattgtt agaacaaatc ttgcaacaat
    30301 cacgtccatt gttgattatt gctgacgatg tagatggtga agcattgcca actcttgtat
    30361 tgaacaaaat ccgtggaaca ttcaacgtag tagctgtaaa agctcctggt ttcggcgatc
    30421 gtcgtaaagc aatgcttgaa gatatcgcta ttttgacagg tggtacagta attactgacg
    30481 atctaggttt agaattgaaa gatgcaacaa tcgaaaactt aggaaatgct tctaaagttg
    30541 ttgtagataa agacaataca acgatcgtag aaggttctgg agaaaaagaa gcaatcgaag
    30601 ctcgtgtgca attgatcaaa aaccaaattg ctgaaacaac ttctgatttt gaccgcgaaa
    30661 aattacaaga acgcttagct aaattagcag gtggtgtagc tgttgttaaa gttggtgcag
    30721 ctactgaaac agaattgaaa gaattaaaat tacgcattga agatgcattg aatgctacac
    30781 gtgccgcagt agaagaagga atggtctcag gtggtggtac agctctagtg aacgttatca
    30841 gcaaagtttc tgctgtagaa gcagaaggag acgtggcaac aggtatcaag atcgttgttc
    30901 gtgctttaga agaaccaatt cgtcaaatcg ctgaaaacgc tggttatgaa ggatcagtga
    30961 ttgttgataa attgaagaac gtggaactag gcactggttt caatgctgct actggagaat
    31021 gggtaaacat ggttgaagca ggtatcgtag acccaactaa agtaacacgt tctgccttgc
    31081 aaaatgccgc ttccgtttct gccttgttat taacgacaga agcagtcgtt gcagataaac
    31141 cagaaccagc agcgccagca gctcctgcaa tggatccatc aatgatgggc ggtatgatgt
    31201 agtaattaag aaagaaccct attttaacag ggttctttct tctttttttg ttttatttta
    31261 agcaaaaggg gcaaagaagg ggcaaaaata tcaaaggcta tttagtttat caattacttg
    31321 ttgtttggct ctttttgtta catggttata aatagacaga gttgtgtttg catcagaatg
    31381 ccctacacgc tccataatgg ctttaagagg tacgcctaat tcagataata gtgaaacgtg
    31441 gctatgtctg aatatatgtg aggatagact tttttctaat tccaactctt cctctacttt
    31501 atggagtata gcgttaaatg agtgcagcgc aagcggagtg ccacttgtag atataaatat
    31561 atattgatcg ggatctgtgg gtctacctgc aagaatattg tcagctatca cactttcaat
    31621 caattctttt gcacgattgg gtaattgcac ttcacgttgc gaataagtat tttttggagt
    31681 tgtttttata gcattatcca ttttcacaga tgtgtaatct aaggtcccat taatggaaat
    31741 tttcccatcc tcatagtcct tcatttgcaa agctagcaat tccccatatc tcaaaccagt
    31801 taaatataga aactcagcta ttatgccgtg tagttttctg cgaggattgg agtatagctg
    31861 tttcagtatt tgatcaattt cttctttctc cagatatttt ttattcatag aaagccttct
    31921 tttttcttct tccacttttt taggatggat tttaactgct agcgcagggt ttctttgaat
    31981 gtattttcta tcgattgcat agtttagcat aacagataga gttgtttttg tttgttttgt
    32041 gtagttcagt gagaggtcac caaacgtata catatcttca attatcttat taatgagtgt
    32101 ctcatcaatg tttctaacga tcgtatcatc gcttatgtgc ttagaaacat gtttcatcat
    32161 cattggaacc ttcaaatagc tagtacgttt aacatgctgc ttataatatt cataccattc
    32221 tttatacagc tcaccaaaag tgatatctga tttattgtaa tcttcgagtg cttctttaat
    32281 ttttttatct agaattttct gagctttttt ccacgcttgt ggtgaattac ttgtaagtgt
    32341 tgtagatttt tttcgtgttt tttctgtata aggatctaca tatctttcaa taaacttgaa
    32401 ttggccatct ttggtctgtt caacccacac ttttaacatc tcctatcatt tgctataata
    32461 ggcataacaa atagacctat ataggtttgt tttctaaaag cacgctctta ctctggacgg
    32521 tggggcgtgt tttttattta ttatacagtt gctttaaaaa tagctgcttt ttcaaatgat
    32581 actaaaggag agaagtggat ttctatttct cccttttggt ttaacgtgaa atgagcagtt
    32641 acatccattt gttttcctgg agcaacagat cccattgtat tttcgttggc atatgtctct
    32701 gattttttgt catctggtcc atatacttcc acatctgtac ctacagggat atctgaatcg
    32761 ccatcatttt ttacggtata agtaattttt actacttgtg caggttggtt ttcttcaaat
    32821 tgatttcttt catcagttag ttctacacta tttagtgtat attcagcatc accaactttt
    32881 acagtgtcac caatcttata gaaggtatcg cttttttctt ctttagaaga tgaagcagag
    32941 gttgaagatt tagttacttt ttcgccacca ttatcacttg ctttattact atctgatcca
    33001 ccatttaaag cagaaccaat aattataatt aaaattactg ctaataccca aaaccatact
    33061 tttttgtaaa aaggtttact aactttatac atttttccgt cttgacccat aacttttttt
    33121 gccatttaaa tattcctcac ttcttgttat aatatatttg cgatctcaga aatgaggtat
    33181 gagtccgtgt gccagcacgg gcttttttaa tgttttgctg aaatcggttt ttttaataat
    33241 tcttgatgta ttcatacata ttgccttgcg taagaatatt ttttcttaaa atagcattgg
    33301 cagacagcat aacaagtgta tctgagctta ttctgaatag aataatactc cataaatttt
    33361 tcgagattaa actgagattc atcagtcagt tcgttctcaa tatagatatt taataaaatt
    33421 agaatagcta ttttatctgc ttctgtttca aattttgagt gaaaagttgt agaagtatcg
    33481 tacaaaactg aatattcaaa atgagaagcg gtgaaatgcg caagttcatg agataaatga
    33541 aaggcttctg cagtttcgcc atatagattt tcattcaaaa aaatgattct aggcttcgga
    33601 taatagaagc caggctcttt catttccatg taaactactt tcaaattgta ttcgctcaac
    33661 atttctttca atttcaaata catacaaccc atcactccaa ctattcattt tcctctaaag
    33721 ctttagcaat tgcaatcgct ttacgcattg tctccttaga tatttctttt ccatcaaaag
    33781 aaaaaacagt atcgttttct gataaatcca catgtttagg ggtttctctt tcttctctac
    33841 ctagaaggta gtctacagag actccaaagt aatcagctaa ccgagctaac tcaatagaat
    33901 taggagtttg tcttttccaa cttcctaaat atccattcga atacccgaaa ttcatttcga
    33961 gctgtctaat ggataatcct ttttcttttg ctaactcctt tattatttcg taagtattca
    34021 ttgataaatc aacctttctg aatgcttaca aaaaaagttt agataaataa tgtaaaaata
    34081 gttgacttat atacattatt aatctatact atgttttgta aacaagttta acaactaaaa
    34141 agacaacaaa aaatactatt gattaataaa tgccaaccgc caagaaagct ttttaaatca
    34201 aagtttatat gtcttattta actatgcatt tagtatagat tattaatcta ataaagtcaa
    34261 cgcagttttt taaaaagttg ttaaatttgt ttacgaatat aaaagaaagg agagaagaat
    34321 atggaaaaaa cagtctcggc aaaaatcgaa gatttgaaat tagacatact gaaacaagca
    34381 aaagtggcga tggaacacgc ggtagataaa gaagactctg ccatggttgc agccatagca
    34441 gagattttag ctcacgttta gtcatcgttt ttacggtctt ctggaagctg accatagata
    34501 acagcataat gaatatctaa ataagcttga acaatttctt ttggcgaaat tgattcgcct
    34561 ttagtagata cttgtgattc gtgatttagc catgcaacaa ctaaatccgc agcgatttca
    34621 aacggcgtta cactttgtgt cagttgccat aaagaatttc acaatatgta tggtcgtggt
    34681 aataatacca aagagcaatt tgaggaattt atggaaatta aaaaatacaa aaaatcattt
    34741 attaaaggag gctaaacaat gaacaaggaa atgagccaaa caatcaaggt acaaaaaatg
    34801 atagatgatc taacacatgg aattgatagc caagccgata aaatcatcaa ggaattgcaa
    34861 ggacaaaaag ttaaggatgc aaaaatgctt ctcaagacta tcaactttga aatgaatcca
    34921 acaaaaagaa agctcgccga tgtgttggaa gaaaagttgg cttccgctat caacgagcaa
    34981 gaattactat ttgaaaccga tacttttaac agctgatgtt ttatagatga cagtttcatc
    35041 cgaatctata tcatcgccga gagaaaagaa tgttgctttt gacaaaaagc cgataagacc
    35101 tacaagcggt cttgatgttg caagagtaga taccccaata tcaccttgca aggtattcac
    35161 gtaaatttga gaagaataga acattccatc tttttcttcg atatttgacg catcatcgta
    35221 tccaacgacc aaagtatcgg caaatatttt gattttttct ccatcaagca tagtaattac
    35281 aaattctggt ttcataaatt ttcacctcct taacaattat ttcagcctgt cacactgata
    35341 aggaaattat accaaagaaa ggaatgagaa atatgaatac accacaaatt tttaatttcg
    35401 agcaaaacga agttcgaact attttagtaa atgatgaacc atattttgta ggaaaagatg
    35461 tagcagacgt tctaggatat tcgaatccgc aaaaagctat tcgagatcat gtggacttgg
    35521 aggataagac gcagaacgat tcgttcaccg tcaatggaac agcagttgtt ttgattaacg
    35581 agtcaggcct ttacagttta atcttaaaat caaaacttcc ctctgccaaa aaatttaaac
    35641 gttgggtaac gagtgaagtc cttccaacaa ttagaaaaac aggtagctat tcaaacgtac
    35701 ctcaaagttt tgcacaagca ttgcgtttag cagcggattt agaagaaaag aaccaattac
    35761 tcgaacaaca aattgccgag tacgaaccaa agattagcta cttagatacg attctttcat
    35821 cgacagatac ggtagctact tctcaaattg cagctgatta cggaatgtcg gcaattgctc
    35881 taaacaaatt gctaaacgag ttaggtgttc agcataaagt tagcggacaa tggatacttt
    35941 accgaaaaca tatgaaccaa ggatacacaa aatcgcacac aagtgagata ccgaaagccg
    36001 atggcggcac taaagttgta atgaatacca aatggacaca gaaagggcga gtgtttattt
    36061 acaacttatt aatcgcagag ggctattacc ctcaaatgga tttagaggaa attggttaga
    36121 aaggagtttt agtatgactg acattgcaga aatcactcaa cgagatagag aaaaaatcaa
    36181 agaatatgtc gaaagttcaa agttcttaac ttacaccatg cttgctgaaa gatttggaat
    36241 tagcaaaagc tacttatctt taattttaaa cggtaaaaag acttctgcag aagcaaacag
    36301 aattatagat tcgattatca ctatgtacga attgtagagg gaggtaaagt cgttggaaga
    36361 taaaatcatt cttacaaaat cagagcttca agaaatgtta aacatcgagt atggaacgat
    36421 aatttttagg agggataata agtgaaaaaa ccaacacttt cagagctgat agaagctgct
    36481 gagaaggcag taaaaccaga cgactggtac cgacaaagtt taatcttgga gaagttccac
    36541 ggcatgtcaa aaactacttt agttgaatac tgcaaggaaa tggaaacaat tcctgaattt
    36601 gcagaaggaa tacttcgtcc aggtcactca accacattta ttcattacca tacttttatt
    36661 tggtttttaa agtggaaaga cgcaaacaaa tatcgtgtaa aaagtttatc gccttcagat
    36721 gttttgaagg aggcaagtta atgggcaaat tcaacagagc actagtattc agcgcaccgc
    36781 taatcatcta cgctttaggc ctctggggaa gcagacaagc gttgataggg acaatcgttt
    36841 acatggtttg gatttttatg gggcttgatg aagctgagca cagagcgaaa aaagccagtc
    36901 gggagggact gactaaaata tgattttttt atttttgttc ctttgggctg ctattgcgta
    36961 cttcatccag ctccttgttg tcaaagttgc tcttatttct tttaggaaga acaaaagaaa
    37021 atagcgctat aaatgaaaga taagtagccg ctacattaaa ctgttctttt gaaaaagtta
    37081 cagtcatcgg aatgataatt gatattgagg aagtaatctt aattaaaaat atagcaatat
    37141 tttcaacatc gcgttggttt ttatttaaca ttgtccaaca tgttttagca acaataaaaa
    37201 taattacaca tgatattagc acacagactg aaaaaagttt tagaaatgcg atgtagtcag
    37261 tgttattgaa ctccttcaaa atactatttt tattcaatgt aataaaagga atttttttaa
    37321 ttgaagacag gaaagataag gtaataaaca gaattatata tatggttaaa gtcgcagtaa
    37381 gaatgtaatt gagctttttc ctcatagaat cacctcactt tctaacgaaa attataccaa
    37441 aaaggagaga agaaataatg caagaattag taattttgaa aaataaagaa gctgtaacta
    37501 cgagcttaca agtggcagaa agtttcaaaa agaaacataa gcatgtacta gaagcaattg
    37561 aatcaataaa aagatcggtc gaaaattcgg ccaatgttga agatgggtcc aattttggac
    37621 agatgtttgt ggaagggaac gagccagact catacggaag aagtcggaga gtttatttca
    37681 tgaatagaga cggattttcc ttgctagcta tgggattcac tggaagtaaa gcaataaatt
    37741 tcaaactaaa atttattgaa gctttcaacg aaatggaaga tgttattcgg aagaatactg
    37801 ttcctcaaac aattgaagac atgatgatct atcaattaga agaaatgaaa gatgttaaaa
    37861 aagatgtttc catgcttaaa gatactatgc gaattagcgg acaacaagag tttgaaatta
    37921 agcaaaaagg aaatatgaaa gttatggaag ttctaggggg aaaagaaagc cgagcttatg
    37981 aagaaatcag caaaaaagta ttctcaaaat tttggtctga atttaaacgt accttttcaa
    38041 tcccaagata tggcgagtta cctcgtaaga gattcgatga tgctgtttca tttattgaaa
    38101 tgtggttacc agaaactgcg atccgcatgg aaatcgatca actgaacaga caacaaagac
    38161 ttttcggtga tgaaaatgaa tagagctgaa gcgctaagaa tagggacggt aattgctaat
    38221 cgctggtgga gacacaataa accaaacatc ctaagccaac aacatatcga aaagcaaaaa
    38281 gcatggcaac aaataaaaaa gtgactcagc cggcaagcat agagtcacaa aacatatcta
    38341 aggagaattt tagcatatga ataaagaact ttccacttta gatcaatatt tgactgattc
    38401 tgaatggggc aagtcgaata tcaaggaaac aaataatcga aaaatcagac gaaatctttt
    38461 gacgaacgaa gaactatcat gtgatcaaga tgacttggga aattttgtga gtatttggga
    38521 tcatgtttac cttatccatc tatcaaaaca ttcaaataaa cctgaataca tctatgtcat
    38581 cgaagatggc ttgattgatg cgctagaaga gtacaacaga gataacttga ttgatatctc
    38641 ttattacgga tcaggtaaga aatacattgc tgaaatggag gcagaatttg atgagtgaaa
    38701 tcaaagggac aacgaacttt gaaaaacttt ttagtcgtaa gttaaataaa attctcaaga
    38761 aaaaaggaaa ttttgattat ttatcttggg ctcacgcgtg ggagattatg aaaaagaatg
    38821 atccacaggc aacggtaact attaatgagt ataaacacta cagggttgtt tctggaactc
    38881 atcaagactt tcttgttgag gaatataaac cttttcttat ggatgaaaca gggacttatg
    38941 tatctgtctc agtaacggtt aaaggacata cggaaacaga
//
LOCUS       DARBET010000021        37293 bp    DNA     linear   BCT 07-NOV-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4027
            SAMEA114569841-rid20112183.denovo.021, whole genome shotgun
            sequence.
ACCESSION   DARBET010000021 DARBET010000000
VERSION     DARBET010000021.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA114569841
            Sequence Read Archive: ERR12203666
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 37293)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 37293)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001972618.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001972618.1
            Genome Coverage       :: 132x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/07/2023 13:18:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,041
            CDSs (total)                      :: 2,975
            Genes (coding)                    :: 2,908
            CDSs (with protein)               :: 2,908
            Genes (RNA)                       :: 66
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 67
            CDSs (without protein)            :: 67
            Pseudo Genes (ambiguous residues) :: 0 of 67
            Pseudo Genes (frameshifted)       :: 26 of 67
            Pseudo Genes (incomplete)         :: 28 of 67
            Pseudo Genes (internal stop)      :: 20 of 67
            Pseudo Genes (multiple problems)  :: 6 of 67
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..37293
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA114569841-rid20112183.denovo.021"
                     /isolate="VRE4027"
                     /db_xref="taxon:1352"
                     /country="Denmark"
     gene            <1..887
                     /locus_tag="SCJ86_001171"
     CDS             <1..887
                     /locus_tag="SCJ86_001171"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016922466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="collagen binding domain-containing protein"
                     /protein_id="HEG1168745.1"
                     /translation="TGEDGKLAIDGLAPGAYQLVETQAPTGYELDATPIEFEIERSQT
                     AVVELTKENRLTPGGVVLTKIDDQSGEVLQGAVFELQDANGTVLQSGLTTGADGKLAI
                     DGLAPGVYQLVETQAPTGYELDATPIEFEIERSQTAVVELTKENRLTPGGVVLTKIDD
                     QSGEVLQGAVFELQDANGTVLQSGLTTGADGKLAIDGLAPGAYQLVETQAPTGYELDA
                     TPVTFKIEKEQEVAIFLTKENRKVADSSDIRGNNPTSSGNKHLPKTGETIISQFILSI
                     LGVLLVFISIKFRKKRNI"
     gene            complement(1311..1481)
                     /locus_tag="SCJ86_001172"
     CDS             complement(1311..1481)
                     /locus_tag="SCJ86_001172"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016615560.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HEG1168746.1"
                     /translation="MPRLTNYFLSCTNPKEIEFLVTDMNATYFQLTKHVLPNAKLVID
                     RLLGFSTPFKEA"
     gene            complement(1675..1854)
                     /locus_tag="SCJ86_001173"
     CDS             complement(1675..1854)
                     /locus_tag="SCJ86_001173"
                     /inference="COORDINATES: protein motif:HMM:NF026041.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEG1168747.1"
                     /translation="MPMVIRLKKQRYTCKNCRSHWNAQSYFIRPRHSISNHVRHKITS
                     LLTEKVSLFFISKSC"
     gene            2241..3353
                     /locus_tag="SCJ86_001174"
     CDS             2241..3353
                     /locus_tag="SCJ86_001174"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321621.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FUSC family protein"
                     /protein_id="HEG1168748.1"
                     /translation="MTVKNKSKKKGRQQVDFYQSLQLDPFILKQKIREAASKKEKCMY
                     ICSLFLRSLFIVLFAICFIIIITTLFESKHKPYAVVLFCMLMSIRFVDFGYKISHSII
                     GLAIVMFSLLVAPYVQLIKWSVMGMLIHFILLSSILMATASDPKMGNASLYGFSYLFI
                     VYSLPKDSLNKNFFTQTSSLLFLFFCWFSVLLYRKHREKNKDKSLFKEIFLKGMYSQE
                     KIWMLIYAFGISLLIVAGEYVPFQRLMWAGFAFSSIVSSYGLMSIGFKERAVDRIIGS
                     LIGCVLFIGISQFIPFNWVGILGGLALGVCSTYRYKTVFNSFGALAITASLFGVPGAV
                     TIRIFENILGVCLGIMYIGVTEILIRKIREKHGLNH"
     gene            complement(3431..3724)
                     /locus_tag="SCJ86_001175"
     CDS             complement(3431..3724)
                     /locus_tag="SCJ86_001175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339899.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antibiotic biosynthesis monooxygenase"
                     /protein_id="HEG1168749.1"
                     /translation="MSITVNILYTSTNGQARKFAEEMVERNIVKAIRAEEGNEKYDYY
                     FPLEDPESLLLIDRWKDEEAIEKHHKSEMMAQINELRKKYKLKMKVERFTDLP"
     gene            3892..4206
                     /locus_tag="SCJ86_001176"
     CDS             3892..4206
                     /locus_tag="SCJ86_001176"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736972.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS cellobiose transporter subunit IIB"
                     /protein_id="HEG1168750.1"
                     /translation="MKKALIICAAGMSSSMMASKTTDFFKQKREEVLVDAVSATEGDN
                     MIKNSDFDLFLISPQTTMFLDKFKKLGESVGKPVVSIPFQAYVPIPTGIQKLAEVIEE
                     NI"
     gene            4227..6206
                     /locus_tag="SCJ86_001177"
     CDS             4227..6206
                     /locus_tag="SCJ86_001177"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345663.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BglG family transcription antiterminator"
                     /protein_id="HEG1168751.1"
                     /translation="MKQKEKLLLRYLIDHKKEYVTSQRLASELLLSDRTIRNYLQRIK
                     ELVEKNGGKIIAKPGYGYQLHILQRLTFDLFLSRQEIVPGYAREPQGFYESEDRQKYI
                     LNQLLLEDAILFMDDLAEELYISRSSLTKDMQEIKERLIPYSLKIVSKHGQGTWIEGE
                     ERNRRHFIMDTFFGNHYGNSLKEYLGQSRFFPDISFEELVIIILDETREAKLKVSDFI
                     IQNLTLHLALGIKRLREGFEIKELGIEQEVSKRVEYQVAQRIVQRIEVIAGVSFPEEE
                     IAYLTLHLMAKSNHKENQNDQELSKELAKVLKRLSQEFHFSLVEDLQLFNGLLDHLSP
                     MMIRLSRGIRIENPLTEEIKKENPKVFDAVKRHFSNMPALKNYTINEDEWAYLALHLM
                     AALEKERAAHKLHALIICATGYGSAQLLKNRVVSEFGKNITVVSVKGYYEINETTLKG
                     IDLIISSIDLSTMFFKIPVLHVSIFLNEDDVRKIRKVVGESIPYHSSDRPIPAFSLQQ
                     KKQIYTEQLSEKFFKTYTYEPKKEEILHDLLQKLSINEEEGYIREMRKQIQQRENMGQ
                     IIFSDTVAVPHPAVPVGIETKVAVALIPSGMQWDQYQEIHFVFLVSPSYVENEGITVV
                     TKAIVRLVDQLKVQQEILAEPIFENFSSKFIKLIE"
     gene            6219..6545
                     /locus_tag="SCJ86_001178"
     CDS             6219..6545
                     /locus_tag="SCJ86_001178"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002285954.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS cellobiose transporter subunit IIA"
                     /protein_id="HEG1168752.1"
                     /translation="MAEKINSETLQVTSFDIIFHSGNARTLIHEAFRSMRKEMFEEVE
                     EKLRQANEEILHAHQSQTQLLKEYASGTKIEMEIIMVHAQDHLMTTMTLLEVAQEMNY
                     LYQKNK"
     gene            6561..7079
                     /locus_tag="SCJ86_001179"
     CDS             6561..7079
                     /locus_tag="SCJ86_001179"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343340.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEG1168753.1"
                     /translation="MQDTVKRKIELEKKQLSLKNMYFNRYLIVRYLTAFFFFMNMQWM
                     ILLLSAKSLGSSLPMILILAILPAVGEQVKLYRKHQTNVPWTKWYFLFQSVFNLLVIP
                     VLFTSGFTFLYPFMANNNGGQLFVFILVVSGIFASAFIQYRLKKISLNQDQQYIRIKQ
                     YEKALYLGKENN"
     gene            7079..8431
                     /gene="celB"
                     /locus_tag="SCJ86_001180"
     CDS             7079..8431
                     /gene="celB"
                     /locus_tag="SCJ86_001180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS cellobiose transporter subunit IIC"
                     /protein_id="HEG1168754.1"
                     /translation="MFDFLQKYLMGPMGKIAQLKIVRAVMAAGMASIPFTIVGSMFLV
                     LNILPLPFPFLEGFFNATFFKVSDLYMIVNTMTMGILSIYFAIVFAYELTSIERDEQK
                     LNVNPLTGALLSVFAFFMCIPELIMTDGKISLIASINETETVVNGIRMGSFVERLGTS
                     GIFTAIIMSIIAVELYCMCVKRNWVIKMPDVVPPGVSRSFTALIPTFVIAFVVMIING
                     LLVALGTDIFKMIAIPFGFVTQLTNTWLGVMVIYFLIHALWIVGIHGANIITSFLTPI
                     VLANMAANAQGANYPLAGEFNNSYVTVGGSGATLGLIIFIAFLAKSDQLKVLGKASLV
                     PGLFNINEPIIFGMPIVYNPYLAVPFFLAPMASATLAYFAIRLEIVKPMLAQMPWPSP
                     VGIGAFVGSGGDWKAAVLAVICAIIAFIIWFPFIKFYDNKLLNEEREKAAELASEKLK
                     "
     gene            complement(8515..10020)
                     /locus_tag="SCJ86_001181"
     CDS             complement(8515..10020)
                     /locus_tag="SCJ86_001181"
                     /EC_number="6.3.2.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376413.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-L-alanyl-D-glutamate--L-
                     lysine ligase"
                     /protein_id="HEG1168755.1"
                     /translation="MSFSLEDIRHLLLKENLLKEFVSTRGWHLDVPEKAEFLQLSYDS
                     RKADASTLFFCKGMNFKEEYLDSAIEQGIQYYVSEQPYENSAVGIIVTDIRKAMALLA
                     MAFYDYPQNKLKVIGFTGTKGKTTAAYFTKAILDHTTNKKTALLSTMNTTLDGKTYFK
                     SHLTTPESLDLYRMMSEAVENGMTHLVMEVSSQAYKTQRVYGLTLDVGIFLNISPDHI
                     SPIEHPTFDDYFYCKRQLILNSKTVVLNHESDYFDLLRETADLFQIPTITYGRSEESN
                     YQVIRSDKGTHGFTLSSHEDRLAICDTAYDILLAGGFNQENAASAIIAAALTGASSQD
                     AQDGLKEARVPGRMDQLIQSNGAHVYVDYAHNYLSLKTLLKFAKNEHPDGRVIVVLGS
                     PGNKAISRRHDFGKVLSETADIAFLTADDPAFEDPKKIAEEINEAITNPDLIVHYEMD
                     RPEAIRRALAESAPQDSVVIAGKGVDPYQKINGVDEPYEGDYAIVKRLIEE"
     gene            complement(10112..10702)
                     /locus_tag="SCJ86_001182"
     CDS             complement(10112..10702)
                     /locus_tag="SCJ86_001182"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319599.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YitT family protein"
                     /protein_id="HEG1168756.1"
                     /translation="MTQEIKKLPVIFLGLFFITVGLNWFLLPHDIASAGVGAIGHLVE
                     TAFSIQRSVTVWSINLMMLLLAAVLLGKSVFFKTVAGSLLFPVVLEIVPKVEMISSHF
                     FSLISGSLLFSLGVYTLYTVGASNGGITIPPIIFHKFFRLPMPQGLLLTNSLIVFLNY
                     YVFGLLETLFVLLSITVSSLFMKILIRLSPVSRKFE"
     gene            10881..12527
                     /locus_tag="SCJ86_001183"
     CDS             10881..12527
                     /locus_tag="SCJ86_001183"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002285966.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide biosynthesis protein"
                     /protein_id="HEG1168757.1"
                     /translation="MDNQRKPALDQLTPQEKMARGSAWMTASNIISRLLGAVYIIPWY
                     AWMGENAKAANGLFNMGYNIYALFLLISTAGIPAAIAKQTARYNSLNEYGTSRRLFIR
                     ALQMMGGLGLLFALFMYIASPWLAHASGGGEELIPTMRSLSIAVLVFPCMSVIRGYLQ
                     GNQEMMPYALSQIVEQVARIFYMLLATFIIMKVMDGDYVTAVTQSTFAAFIGMLASIL
                     VLLYFLKKHQAYTSAFIHYSENKINIATKELLLDTVKEAIPFIIVGSGVTIFKLVDQF
                     TFMKIMSASTEYSNAQLLDLFSIFSANPDKLTMVVIALATSIASTGLPLITEAVTLKD
                     RVGLAKLTSGNLQLFSFFMFPATFGVMLLAYPLNTLFYTPDSLGSNVLIQASFVGLFL
                     GLYMLVSNMLQGMFENKAAINYLVIGFLVKLLLQYPAIRLFEVYGPLLATMIGFAVSC
                     TLILKRIHEVARFKPSFVWRRTLLIFILTLIMLLASWITKMIFGAFLNEDSKFQSLLL
                     ILVVAGIGALVYGYLALKIRLAERLLGPGIARLRHKLKIK"
     gene            12576..13295
                     /locus_tag="SCJ86_001184"
     CDS             12576..13295
                     /locus_tag="SCJ86_001184"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374174.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rRNA pseudouridine synthase"
                     /protein_id="HEG1168758.1"
                     /translation="MRLDKFLADMGLGSRKEVKGLIKKGTIEVNGQVVKSDKAQVNEF
                     EDAVTYLGEIITYQKDFYYILHKPAGVISATQDNHDQTVIDLLEDQDYREDLFPVGRL
                     DKDTEGLLILTNDGVFAHQLLSPKKHVEKEYLAEVSGIMTEEDIRLFAEGLIIDGDEQ
                     TLPAKLQIDQVDQETETSIIRLILHEGKFHQVKRMVKAVGKEVLYLKRIRMGKFWLPK
                     DLNKGQYRPMTKEELEQVREK"
     gene            13774..14721
                     /locus_tag="SCJ86_001185"
     CDS             13774..14721
                     /locus_tag="SCJ86_001185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005877947.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HEG1168759.1"
                     /translation="MKKRKGFNHFFMNLWRYKALVLMAVPGMLWMIFFFYIPVMANVV
                     AFKNFHISADGFLASLRESPWVGWENFKFLFSSDQAFLITKNTILYNVAFILLNLLIS
                     VVFAIIMSELRNKRLVKVYQTMSLLPYFLSWVIISYFVYAFLSPDKGIFNQWITGHGG
                     EAVNWYNEPKYWPFILVFIGTWKGIGYNSIIYFASVMGIDPTYYEAAMVDGASKWQQI
                     KHVTIPQLIPLMTILTILAVGNIFRADFGLFYNIPRNSGALYDVTQVLDTYIYNGLTS
                     TGDFGMTAAAGLYQSVVGFVLLMITNTIARRFDSDSALF"
     gene            14733..15653
                     /locus_tag="SCJ86_001186"
     CDS             14733..15653
                     /locus_tag="SCJ86_001186"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339904.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HEG1168760.1"
                     /translation="MKKKKVERVQVRSFNKTANFFSNIMIALFAISCILPFIFVIAIS
                     FTDESILSTHGYSFWPEKFSIFGYTYLFDQMQDKIFQALFVTVLVTVLGTLINSTATS
                     LYAYAISRSNFPFRRFFTVFCLITMLFSPGMVANYLVMTNLLQLKDTIWALILPMAVS
                     PFNIIVMRTFFKRSVSDSIIESARIDGASELRIFVQIVLPLAVPGIATISLFAALGYW
                     NDWFNALLYIQDDKLVPLQYLLMKIQSNIQYLTQNSGAGSQLTGGLASVPGESARMAI
                     VVISTLPIAISYPFFQKHFVKGLTIGGVKE"
     gene            15694..17151
                     /locus_tag="SCJ86_001187"
     CDS             15694..17151
                     /locus_tag="SCJ86_001187"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HEG1168761.1"
                     /translation="MKLWKKLAFTGVSALLLGTLAACGGSGSKDQAEASNSDTLQMYQ
                     IGDKPDNFDQLMEVANKRIEKEIGVKVNINYIGWGDYEKKMNVIISSGENYDIAFANN
                     YVSNAQKGAFADLTELAPKYAEKAYNDLDEAYIKGNLVNGKLYAFPVNGNVFAQQVLT
                     FNKALLDKYDLSIDGIQSYADAEKVLKEFHEKEPNTAAFAIGQGFKVQGNFDYPLGNT
                     LPFAIDLDGDDSKIINQYDNETYIGLLRTMHSWYKQGLIPSDAATSNKDYPLEGNTWL
                     MRGETQGPYDYGDTILTNAAKQELVSKAITVPLKSTAQAQMANFVVSNTSKNKEKSVE
                     LLGLLNSDPELLNGLVWGIEGEAWEKVDGSDDKIKLLDGYQPNTHMSAWNTGNNKILY
                     TQESITDDMIAKRDQSIADAETSPILGFSFNTDSVKTELSNISNVMNQYLDGLNTGTV
                     DPDETLPKLKDALDKAGYDKVLKEMQKQYDEFRQE"
     gene            17496..18110
                     /locus_tag="SCJ86_001188"
     CDS             17496..18110
                     /locus_tag="SCJ86_001188"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010782528.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF624 domain-containing protein"
                     /protein_id="HEG1168762.1"
                     /translation="MLGKVLEELFIRIWVIIKLTLYFWIYTFAGGIIFGLGAAWKTVN
                     ELFYLYGFEYKEITIKRGWNIYKRNFLRGNLLFSLFLSGTALLSYNLYLSVQIKGLIF
                     LAIDFILLFALLCLFATYQYSMILDSEYTISVPNLLKLSFISVFSSFSSFLKIIIGSG
                     IILGVTWQFKGLILFGVIGLLTVWNGTMTTHWREELDKQLESYE"
     gene            18103..19827
                     /locus_tag="SCJ86_001189"
     CDS             18103..19827
                     /locus_tag="SCJ86_001189"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292278.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HEG1168763.1"
                     /translation="MNKFRILFSKGTLLNRLLRKYAVIMIGVTMTATVIFSIHTWEQN
                     QKQAENMTSDAVQSTSRMLNDKTTLSRIIKNQLVGNSEKIENVTTYLTKPIDQYLMYV
                     YEQQNSTDELVSFPNQIKDLYANYEELSAIYIVLNQLPEYYESSRENKGGEIKKGTPK
                     LLDAFYIKLPITQGGAESGSIFIGFQKKELDMILENLTSFNGLSLYMISGTTNRLYTF
                     HDNKIAKETFERQEMIITESLKKNSQLPIDDLEKNNFVQHQELSDDYRILAVLDKKSV
                     KIETGKNLAPLIIGIIILDNILLIFLYKTFKRYSQQVSIVMQAMDQTAAGNLETRIDI
                     TNTEYELRELSIGINEMLDSINQFVEDIYKLEIKQQDAHMRALQAQINPHFLYNTLEY
                     IRMYAISEGSEELADVVYAFSALLRNNTNQEKTITLKEELDFCEKYVYLYQMRYPNRV
                     AYHFMIDPDLEKIEVPKFVIQPLVENYFKHGIDFTRFDNALSVKVLQEGKRVRIIIKD
                     NGKGMTEKRLKQIEEKLSHPKVELHGSIGLQNVNERLRASFGSSYYMSLENNETGGLT
                     VSITFKEG"
     gene            19824..21275
                     /locus_tag="SCJ86_001190"
     CDS             19824..21275
                     /locus_tag="SCJ86_001190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628234.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HEG1168764.1"
                     /translation="MSVYQVLLVDDEYMILNGLKKIIDWQSLGFQIVATAENAKEGLA
                     VLEQRQIDLVVTDVTMPEINGLEFIEAAQKERHNFEFMILSGYQEFDYLKGGMQLGAV
                     NYLMKPVNKFELVESLKKIKTRLDQQNEQKNQQEIYQELLFSQWLNEELDEISEEEII
                     GQISEKQHRIVLIQLARTHDTLINRWLQERQQIFYYQRNYGDRVLITLLLEADEVDGF
                     CCFVQENFPDQEWLISIGEETDSLDKIPETFQQAKDNLQLHQFYGDKDEHVFYAVQAE
                     TKDQTIDFSSFRRVLQNKQLEVAQKMITDFFEQFQLAAMMPEDIRYSSFLLFMEIQRE
                     LIDLEDEEYLQGIEKIHQAKTVQELQQLLLSFIKKHQRQKKYSDNVEKVIVILHQHYQ
                     EPLTLKEVSENLHLNVMYLGQLFKKETKKSFSAYLNHLRMEKAKQLLLHSNQNINEIA
                     SEIGYNNTTYFSKLFKKIVGRSPKEYRENSGES"
     gene            complement(21748..23901)
                     /locus_tag="SCJ86_001191"
     CDS             complement(21748..23901)
                     /locus_tag="SCJ86_001191"
                     /EC_number="3.2.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GH92 family glycosyl hydrolase"
                     /protein_id="HEG1168765.1"
                     /translation="MNIGQIDTRHATANQPNFSNGNCQPYTGVPFGMNYFAPQTTDQN
                     GSWWFHPDDRVFQGYRLTHQPSPWMGDFSHMLFTPINGKLQENTLFHAQSSYRPEESI
                     FCPTHLSICQLRYGICSTLIPSMYGGILSIDYSKNDSGLLLSFPGRHQLFVIDPYTVE
                     GKITHFAGSEDPDFSFYFILRFEYPLTIDELAVSNKNSDSIPLYFEQTKKQTIRFGTS
                     FISSEQAAYNLELEQNQSKETYLAHSQEQWQSYFDRIQLTHHDQQQLRTFYHNLYRVF
                     LFPQTFYEIDPSGQKIHYDTTSRSIKPGVLYTNNGFWDTYKTVYPLYSLIAVEKYEEM
                     LEGFLNSYRESGYLPKWLSPDERGLMPGTLIDAVIADAAVKDIRRDLMPEFLTAMEKG
                     ATVQSKHPNYGRQGTEDYLTYGYVPNHYHESVNHTLDYAYSDFCISQVAKVLEEEEMT
                     KKYQQQSLNYQNIFDYESGFMRSKDKDGKFREPFSANRWGQDYAEGSAWQSSFAVYHD
                     FSGLIGKYGGSERYKEKLIELCNQPPTFDPGGYGFEIHEMSEMAAIEFGQLAISNQPS
                     FHYPYLFSYIGQPEMAQPLLKQLMTQTFDDSPMGYPGDEDNGSMAAWYIFNSLGFYPV
                     TPGTGEYVIGMPLIDKAVLLLSNGKELTIEATPNRPQQQFIHKIKRMDREYTKLYFTH
                     QDLLKGGTISYQLGIIPHPKKYQAKDLPFSLSNYN"
     gene            complement(23903..24949)
                     /locus_tag="SCJ86_001192"
     CDS             complement(23903..24949)
                     /locus_tag="SCJ86_001192"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307967.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HEG1168766.1"
                     /translation="MTQPLYQTIFNDLQTAILTGELPVDSQLPTEKELSRTYKVSRIT
                     SKRALTELEQLGLIYRVRGKGSFVKSASPEQILASPTKTRRILFLLPYVADLSVGDFS
                     KGLNPVMQDKQIDVMMTTLDFLQNKTAKELINEFDGLIYYALDTEQHLDLLFELSLKE
                     FPVVILDKKIYELPFPTVLSDNFQGGALAASYLIEQGHSKIAYLFGTQTHPQSVRQRY
                     LGYLEALDKAGLTFHTSLDDKSATLDELLSYITKYEVTAFVCENDVVAIQTMNLLRQH
                     GYHIPEDFSIIGFDDIQAAALVDPPLTTIAQDFEQLGKLAGDALVTWLETKDRPSDSK
                     FPVSFIKRQSTKEK"
     gene            25099..26388
                     /locus_tag="SCJ86_001193"
     CDS             25099..26388
                     /locus_tag="SCJ86_001193"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 125 protein"
                     /protein_id="HEG1168767.1"
                     /translation="MVYKDIPKSVQKFMEDITQKCGDIHADWAKNFTAAFANTLLTTV
                     KKQNDGTTFLLTGDIPAMWLRDSTAQVRPYLVIAKEDEELADMISGLVKKQFFYINID
                     PYANAFNEEANGAGHQTDHTKMNDWIWERKYEIDSLCYPIQLAYLLYKNTGKTDQFNQ
                     SFIEGIKKILDVFKTEQDHEHSPYTFVRDTTRKEDTLINDGRGAKTAFTGMTWSGFRP
                     SDDACRYGYLVPSNMFAVVVLGYVEEIFQTILDDKEIVQQTQKLKKEIQEGIQNHGRT
                     VNQKGQTIYAYEVDGMGNATVMDDSNVPNLIAAPYLGYCSEQEEDYLQTRRTLLSKEN
                     PYFYEGKYAKGIGSSHTPEKYVWPIALAMEGMTTSDKSEKERILNLLVATDAGTHLMH
                     EGFDVNNPENYTREWFSWANMMFCELVMDYFDIRVEK"
     gene            26401..29103
                     /locus_tag="SCJ86_001194"
     CDS             26401..29103
                     /locus_tag="SCJ86_001194"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324610.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-mannosidase"
                     /protein_id="HEG1168768.1"
                     /translation="MKKKVYIISHSHWDREWYMAYEQHHMRLIELIDDLLELFEVDPS
                     FNSFHLDGQTIILDDYLQVRPEKRQAIQQAINEGKLRIGPFYILQDDFLISSESNVRN
                     MLIGMEESRKWGTPVMLGYFPDTFGNMGQTPQLMKQAGISAAAFGRGVKPIGFDNQVL
                     EAENYSSQYSEMWWKGPDQTAIFGLLFANWYSNGNEIPVEKEAALAFWKQKLADAEQY
                     ASTNHLLMMNGVDHQPVQKDISKAIHLANELFPDYEFIHSNFTDYLEAVQKDVPEDLG
                     SVEGELTSQETDGWYTLANTASARVYLKQWNTKVQRQLENITEPLATMAYEVSGNYPH
                     DQLDYAWKTLMQNHPHDSICGCSVDSVHREMIPRFEKADEVGKYLAQDSLEQLTAAID
                     TTGFPKDSFPFVIVNTAGMDKTGEAEITIELERKRFAEGIPEQLYQELENLPKRKYHV
                     ETKSGATIPAILSEETVQFGYDLPKDRFRVPYMARMIKVTLPLENMPAFSWETFALVE
                     GEAKAEEKETMIHQSGRIIENGPLHLTIEKNGTITMEDRKNKRKLNNLHIFEDIGDIG
                     NEYIFKQPFCDQPILSSNKENSEVKVLVDTPEIAKISILQEMEIPVSADERLEKEQQM
                     VVEFRYRKAERSKEKRILQIKTIMTIRKDSKKIDFETTLDNQMKDHRLRVLFPTKLHV
                     EHHEADSIFEVVKRPNHVSKSWENPTNPQHQQAFVNIHDEEYGVTVGNFGLNEYEVTE
                     DGQIAVTLLRSVGELGDWGYFPTPEAQCLGEHRFNYSIELHGPEEKFSTYLHAYAAQI
                     PFSTQQIKHHEGTLISKQQYLTIKSETFAITALKRSKFSDKVVVRGFNMSSHLEKLEI
                     TKDNGKTVILNLLEEPTKQAVVPIIQPYEIRTIGFEEENECMAE"
     gene            29088..29945
                     /locus_tag="SCJ86_001195"
     CDS             29088..29945
                     /locus_tag="SCJ86_001195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family protein"
                     /protein_id="HEG1168769.1"
                     /translation="MYGGIEAGGTKFVCAVSDGGEILEKLSIPTTTPEETLDQVFEFF
                     DRYELKGIGIGSFGPIGIDKTKDNYGYVLATPKKGWAQFDFLGTIKERYNIPAAWTTD
                     VNAAAYGELKQGAAQGKNSCIYLTVGTGIGAGVVINEEIFSGIAHPEMGHIWVKRHPD
                     DHYEGTCPYHKDCLEGLAAGPSIEARTGIKGQDLPQDHPVWAIQAYYIAQALINYTLT
                     LAPEKIILGGGVMNQDHLLQKIRQQFVELMGGYMETPQVSEYIVRWGLPNESGIIGSL
                     LLAEHEHQK"
     gene            complement(30016..30915)
                     /locus_tag="SCJ86_001196"
     CDS             complement(30016..30915)
                     /locus_tag="SCJ86_001196"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321119.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RluA family pseudouridine synthase"
                     /protein_id="HEG1168770.1"
                     /translation="MVQLVCNCTERSTLVKITITLPMNHPETTIRDLLEKEWLVPRKV
                     RHFLRIRKNVWINQQPALFHHEVKGNDQITLLFEETDYNYQEVQLGDAKHIRVLYEDE
                     HLIIVDKPAGMKTHPNEPTENSTLLNHLAAYLDTKQQRPYVVHRLDKETSGAVLFAKN
                     PFVLPILGRMLEQKMIYRRYQAIVWGEIAKDMTITNKIGRDRHDRRKRVVDDKNGQSA
                     LTHVTVNKVLSEQTQVYCVLETGRTHQIRVHLAHKGHPIVGDPLYQNRTAERLMLHAL
                     ELHMIHPFTQEKIVGKALPGLWE"
     gene            31084..33255
                     /locus_tag="SCJ86_001197"
     CDS             31084..33255
                     /locus_tag="SCJ86_001197"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002285990.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PBP1A family penicillin-binding protein"
                     /protein_id="HEG1168771.1"
                     /translation="MDFQSILEKIKAALISFWRWIRPYLGQFHRWRKRIWKKYHVNKL
                     ILLIGLICVLVTSIYLFILAKQANVETLKSGLSQSTVIYDKKNEEAGTLYAQKGTYVE
                     LNAISPYIQDAVISTEDRNFYEHHGFDIKGIARAAVRMVLSGGTSGGGGSTITQQLAK
                     NAYLTLDQTFDRKAKELFLAIEIEKKYSKEEILTMYLNNAYFGNGVWGVQDASRKYFG
                     VDATNVTLSEAATLAGMLKGPGIYNPIDHPKNANDRKNTVLSVMEENGKITKEQEQAE
                     SQTDISAYLNDTYDNSDSGYRYPYYFDAVIDEAVNEYGLDEEDVMNKGYKIYTALDQD
                     YQKQLEATYQNSALFPANASDGAMVQSASVALDPNTGGVQALVGRRGDHVFRGFSFAT
                     QMRRSPGSTIKPLSVYTPALEAGYKPSSILKDEPQSYYDAHNFDGTYQGEVPMYQAVA
                     QSLNLPAVWLLHEIGLQKGYDKAEEFGLPLAKSDKYYGLALGGLEKGVSPLIMAGAYS
                     AFANDGQMYTPHLITKIVDSTGAVIVDNTNKKPKQVISKETADEMTSMLLGTFSNGTA
                     AAANPAGYTIAGKTGTTETNFDATKANDQWMIGYTPDVVISTWMGYEESSKLHYLEGT
                     SGNVVGKVFKSAAEGILPYTSKTGFSVADAYATGGQVVAADQVGNTGNSNGESGNWQD
                     NLNQYGEQAKEGLKNFGDMVKEGVQGIGEAAKDLWRKYQGE"
     gene            33323..33664
                     /locus_tag="SCJ86_001198"
     CDS             33323..33664
                     /locus_tag="SCJ86_001198"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307974.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YlbF family regulator"
                     /protein_id="HEG1168772.1"
                     /translation="MSNIYDSANQIEREIRELPEFKALKEAYEKVQANEEAHKLFKDF
                     QAMQIELQEKQMSGQEFSDEDAMKAQEMAVKIQSEEVINDLMQKEQGFSTIINDLNRI
                     IMTPVRDLYSE"
     gene            complement(33940..35694)
                     /locus_tag="SCJ86_001199"
     CDS             complement(33940..35694)
                     /locus_tag="SCJ86_001199"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002285995.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEG1168773.1"
                     /translation="MEERIQKSYQKYHANAFQTLLHLYQGNYWRFAMSGLFFIIKHLP
                     SWLMPIAIANVINAASSKDSSQLQAIYFNAFLMACLIGQNILTNYFHVKYHSTSIRQV
                     EAGLRATLIKKIQELSIPYQKELQSGRIQSKIIRDVEAIQTLSSQVFVSGLNIAVNLF
                     VALGITLYKSPVVFMFFLLTVPLAAFVTLFFRKRIRQSNSDFRQEMEETSAKVIEVIE
                     LAPIARAHGLEKHETTRLNKHFTQIYEKGFHLDILQSVFGSVSWASFQIFQLICLIFT
                     GILALQGRIQPGDVVMYQTYFTTIVNSVSGMITLIPTISKGMESITSIGELLIAGDVE
                     TYHGKKKIQHLDGNFRFEDVSFLYPDSLKPIIDHFSLEVKAGETIAFVGPSGSGKSTL
                     LNLLIGFIQPTSGKIFLDDQPLDELDMRSVRNYLAVVPQTTLLFSASIKENITYGLKN
                     VSKERLDEVIEAAQLSSLIDQLPDGLDTLVGEHGNKLSGGQKQCISIARALIRQPKII
                     LLDEATSALDNQSEKKIQQALNYLTETPTTFIVAHRLSTIKEADKIVVIDEGKIVEIG
                     TYEKLLAQKGYFYRLKNE"
     gene            complement(35705..36541)
                     /locus_tag="SCJ86_001200"
     CDS             complement(35705..36541)
                     /locus_tag="SCJ86_001200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344933.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphate isomerase/epimerase"
                     /protein_id="HEG1168774.1"
                     /translation="MFPLNLGIRAHDLDVRNREEIVQKVSQLHLSHIQFAPQKAFPEQ
                     LKLTDMTLGSAAYFGEYFQKNNIELSILGCYINLSSKNQEVRAQALATFKQQIALCSA
                     YQAKMIATETGSVSVGYTKENFTEEAYQIARNSIIELVAHAENFGVTVAVEAGINHPI
                     YNYQLAKRLIDEVASPNLKIILDCANLMHKENHADQEKVIRGALDNLHGYITALHLKD
                     YIMENGKIRMVPVGKGCLDFRPVLHYIKYERPLMYATLEATPEIYVPEAIEHLQFIYQ
                     DC"
     gene            complement(36543..37226)
                     /locus_tag="SCJ86_001201"
     CDS             complement(36543..37226)
                     /locus_tag="SCJ86_001201"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310846.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhamnogalacturonan acetylesterase"
                     /protein_id="HEG1168775.1"
                     /translation="MPTIHIAGDSTAAEKDENKRPETGWGEKISAYFSPEIQINNQAK
                     NGRSTKSFIQEGRLARLASQFQPNDFLFVQFGHNDQKIQEERGTLPFGSYLKNLQIFF
                     ECAKKANVQMVLLTSVTRRDYLENGTLNPDILGDYPKAMRAFAEKHHIPLLDVFSRSQ
                     ELFQTFSKEETKKFYLHLMPDTCKNYPEGLKDNTHFSPEGADKVARIIVELIKESRLP
                     LANYLQKGV"
BASE COUNT        12033 a         6559 c         7654 g        11047 t
ORIGIN      
        1 ctacaggaga agatggaaaa ctggcaattg acggtttagc accaggagca tatcagctgg
       61 tagaaactca agcgccaacg gggtatgaat tagatgcgac accgatcgag tttgagatcg
      121 aaagaagtca gacagcggtg gtagaattga cgaaagaaaa ccgacttaca ccaggcggtg
      181 tcgtgttgac aaagatcgat gaccaaagcg gggaagtcct gcaaggagct gtttttgagt
      241 tacaagatgc caatggaacc gttctgcagt caggtctaac cacaggagca gatggaaaac
      301 tggcgattga cggcttagca ccaggagtgt atcagctggt agaaacccaa gcgccaacgg
      361 ggtatgaatt ggatgcgaca ccaatcgagt ttgagatcga aagaagtcag acagcggtgg
      421 tagaattgac gaaagaaaat cgacttacac caggcggtgt cgtgttgaca aagatcgatg
      481 accaaagcgg agaagtcctg caaggagctg tttttgagtt acaagatgcc aatggaaccg
      541 ttctgcagtc aggtctaacc acaggagcag atggaaaact ggcgatagat ggcttagcac
      601 caggagcata tcagctggta gaaactcaag cgccaacggg gtatgaattg gatgcgacac
      661 cagtaacttt caagatcgaa aaagaacaag aagtggcgat ctttttaaca aaagagaata
      721 gaaaagttgc tgatagctca gatataagag gaaacaatcc aacaagcagt ggaaataagc
      781 atcttccaaa aactggagaa accatcatta gccagtttat attgtcaata ttgggtgtgc
      841 tacttgtttt catcagtata aaatttagaa agaaacgaaa tatatgagct aaaataaaaa
      901 attgaataag catagttttt aaaatcaact agtacccatc aattgaatgg aaactaccga
      961 ttttagaagg ggtatacaaa caaatattag tatcggagta gagaaggttg aacccaacca
     1021 gtaaataagt aaagacaatt ttgtgctgcg ttgagaagtg aaaaataaac ctttgtagta
     1081 atactagtta ataaaaaata tacaaaaaca ctgcgaaaat cattcttgag tttcgcagtg
     1141 ttttttgcct aatgactcaa gtttattggg taatactttg attttctttc tacaaataat
     1201 cagcgatcag ttgtaatttt ttctctagtt ttaatggctc tttgtcaata aggagaaaaa
     1261 caagtcgggt cttttgtctc ggaaagcttc aaccaactcg tgaataaagg ttacgcctcc
     1321 ttgaaaggcg tggaaaaacc aagtaatcga tcaatcacca atttcgcatt tggtagaaca
     1381 tgtttggtga gctgaaagta ggtggcgttc atgtctgtaa ctaagaattc tatttcttta
     1441 ggattagtgc agctaaggaa atagtttgtt aatcgaggaa ttttttgtgt aggtaaattt
     1501 tctcgattaa tttttctgtc tcgccatccg cggaaataaa actcagttta tcctcaacgg
     1561 aaacgtgaga acgaaattca caaccctcaa cactcaagac agaattcttt tggaagactt
     1621 tggtaaatag cttctcaact ctttcaatgt gcgaataacg gttgtcaaag atacctaaca
     1681 acttttcgaa ataaaaaata acgacacttt ttcagtcagt aaagaagtga ttttatgtct
     1741 gacatgattt gaaatcgagt gccttggacg gataaaataa ctttgagcgt tccaatggct
     1801 tcgacagttt ttacaggtat agcgctgctt ttttaggcgt ataaccattg gcatatgatt
     1861 atattattca aagtggacaa tggtttcctt cttcttgttt ttcaccacca ttaatttcct
     1921 atttccatct accacagcag aaccacaatt tctacagaca catggagcag ggaagagaac
     1981 tgcagcaacg atcaatgttt ttttcttctg taaggtttcg taagagacct ctgtaatcat
     2041 caaatttttg tctattgatc tcagaatttt tttgatagaa ccattcataa agcatatcgt
     2101 tctctcagtt gtttattttg tggtgattta atcatacaag aaaacgatat gtttttttat
     2161 aaaaacatga ataggaaaaa tagttgacaa gaaaatgaca acgccatatg atgttccata
     2221 gtatttacaa cctgtcatag atgactgtaa aaaataaaag taaaaagaaa ggacgacaac
     2281 aggtggattt ttatcagtct ctacaactag atccgtttat tttaaaacaa aaaatacgag
     2341 aagcagcttc taaaaaagaa aagtgcatgt atatttgttc attgttttta cgatcattat
     2401 tcatcgtatt gtttgccatt tgttttatca ttataattac aacattattt gaatcgaagc
     2461 ataaaccata cgctgtggtt ttattctgta tgttgatgag tattcgtttt gttgactttg
     2521 gttataagat ttcccattcg atcataggtt tagcaatcgt catgttctca ctattagtag
     2581 caccgtatgt ccagctgatc aaatggtcag ttatgggcat gttgattcat tttattttac
     2641 taagcagcat attaatggca accgctagtg atcccaaaat ggggaatgct tctttgtacg
     2701 gcttttcata tctctttatt gtttattcgt tacctaaaga ttcgttgaac aagaatttct
     2761 ttacacaaac aagtagctta ttatttttgt ttttttgttg gttttcagtt ctcctttaca
     2821 gaaaacatcg agaaaagaac aaagacaaaa gcttgtttaa agagattttt ttgaaaggca
     2881 tgtattcgca ggaaaaaatt tggatgctca tctatgcatt tggcattagt cttttgatcg
     2941 ttgcaggaga gtatgtccct tttcagcgat tgatgtgggc agggtttgca ttttcttcta
     3001 ttgtatctag ctatggattg atgtcgattg gtttcaaaga acgagcagta gaccgaatca
     3061 ttggctcatt gattggatgt gttttattta ttggtatcag ccaatttatt ccttttaact
     3121 gggtcggtat attaggtggt ctagctttag gtgtttgttc tacctataga tataaaacag
     3181 tttttaattc ttttggtgct ttggcaatca ctgccagttt attcggggtt ccaggagcag
     3241 taacgatccg catttttgag aatatccttg gggtatgttt aggaatcatg tatattgggg
     3301 tgactgaaat acttatccga aaaataagag aaaaacacgg tttgaatcat tagatgagat
     3361 cgaataaaaa ataaggaaaa agtgaagata cacatggatg ataaattgct atcattccat
     3421 gtgtatcttt ttacggtaaa tcagtgaaac gttcgacttt catttttaat ttatactttt
     3481 tacggagttc gtttatttga gccatcattt cggatttgtg atgcttttcg attgcttctt
     3541 catctttcca acggtcgatc agtaaaagag attctggatc ttctagtgga aagtaatagt
     3601 catatttctc gtttccttct tctgcacgaa tcgctttgac aatgtttctt tcgaccattt
     3661 cttctgcaaa ttttctggct tgtccatttg tacttgtata caaaatattc actgtgatac
     3721 tcactaaatc ccctcctgaa aaaattctat cactagtata tcacggtgcc agaaattatt
     3781 attcaagata tgatacacat tttttccgca atgctacgga aatatatatt ttgaatagaa
     3841 aacgcttaca agatatgctt ggtttataaa aataaaagga gtgattgatt tatgaaaaaa
     3901 gcattgatta tttgtgcggc aggaatgtca tcctcgatga tggcctcaaa gacaacggat
     3961 ttctttaagc aaaaaagaga agaggtactc gtagatgcag tttcagctac agaaggcgat
     4021 aatatgatta agaatagtga ttttgattta tttctgatca gcccgcaaac gacgatgttt
     4081 ttggataaat tcaaaaaatt aggagaatct gttggaaagc ctgttgttag tatccctttc
     4141 caagcgtatg tcccaatccc tacaggcatc cagaagttag cagaggtgat cgaagagaac
     4201 atatgatgga ataggagtgg atgataatga aacagaagga aaaattgctg ttgagatatt
     4261 tgattgatca caagaaggag tatgtcacca gtcaacgttt agcttctgaa ttgttattat
     4321 ctgatcggac aatccgtaat tacttgcagc ggatcaaaga gttagtagaa aaaaacggag
     4381 gtaagatcat tgctaaacca ggttatggct accaactgca tattttacaa agactgacat
     4441 ttgacttgtt tttgagtcgg caagaaatag tacctggata tgctagagaa ccgcaaggat
     4501 tttatgagtc cgaagatcga caaaagtata tattgaacca attgcttttg gaagatgcca
     4561 ttctttttat ggatgatttg gcagaagagc tttatatcag tcgttccagt ttgacaaagg
     4621 acatgcaaga aatcaaggag cgattgattc catattcact aaaaatcgtt tctaagcatg
     4681 gtcaagggac atggatcgaa ggggaagaaa gaaatcgacg gcattttatc atggatactt
     4741 tttttggaaa ccattatgga aattcattga aagaatattt agggcaaagc cgttttttcc
     4801 cagacatcag ttttgaagag ctagtcatta tcattctcga tgaaacaaga gaagctaagt
     4861 taaaagtttc ggattttatc atacagaatc tgactttaca cttggctttg gggatcaaac
     4921 ggctccgaga agggtttgaa atcaaggaat taggcatcga acaagaagtt tctaagcgag
     4981 tagagtatca agtggcgcag cggatcgttc aacgtattga ggtaatcgca ggtgtatcgt
     5041 ttcccgaaga agaaattgct tatttgacat tacatctcat ggctaagtcg aatcacaaag
     5101 aaaatcaaaa tgatcaggaa ctatcaaagg aattagcgaa agttctgaaa agattgtctc
     5161 aggaatttca tttttcatta gtagaggatc tccagctttt caatggacta ctcgatcatt
     5221 tgtcaccgat gatgattcga ttgagccgag gtattcgcat tgagaaccca ttgactgagg
     5281 agataaagaa agaaaatccc aaagtctttg acgcagtaaa aaggcatttc agcaacatgc
     5341 cggctttgaa aaactatacc atcaatgaag atgaatgggc ctatttggct ttgcatctca
     5401 tggcggcatt ggaaaaagaa agagctgcgc ataaactaca tgcattgatt atctgtgcaa
     5461 cgggttacgg cagtgcccaa ctattaaaga atcgcgtagt cagtgaattc ggtaaaaata
     5521 ttacagtggt aagtgtaaaa ggctattatg agatcaatga aacaacttta aaaggaatcg
     5581 acctgattat ttcttctatt gatctttcga caatgttttt caaaattcca gttttacatg
     5641 tcagtatttt tttgaatgaa gatgatgttc gtaaaatacg aaaggtagtt ggagaatcta
     5701 taccctatca ttcctcagat agaccgatac cagcattttc tctacagcaa aaaaagcaga
     5761 tttacaccga acagttgtct gaaaaatttt tcaagactta tacgtacgaa ccgaaaaagg
     5821 aagaaattct tcatgatttg ctgcaaaagc tgagcataaa tgaagaagaa ggatatataa
     5881 gagaaatgcg aaagcagatc cagcaacgtg aaaatatggg acaaatcata ttcagtgata
     5941 cagtggcagt cccacatccc gctgttccag ttggaatcga aacaaaggta gccgttgcat
     6001 tgattccttc tgggatgcag tgggatcaat atcaagaaat tcattttgtt tttttagttt
     6061 cgccttctta tgtcgaaaat gaaggaatta cggtcgtgac taaagcaatc gttaggttag
     6121 ttgaccagct aaaagtacag caggaaatct tagctgaacc tatttttgag aatttcagca
     6181 gtaaatttat caaactgatc gaataaggag gaaaacagat ggctgaaaaa ataaacagtg
     6241 aaacactaca agtgacttct tttgatatca ttttccatag cggaaatgca cggacactga
     6301 tacatgaagc atttcgctcg atgcgtaaag agatgtttga agaagtagaa gagaagttgc
     6361 gacaagcaaa tgaagagatt cttcatgcgc atcagtcaca aactcaactt ctgaaagaat
     6421 atgccagcgg gacaaaaata gaaatggaaa ttatcatggt ccatgcacaa gatcatctga
     6481 tgacgacgat gacattattg gaagttgcac aggaaatgaa ttatttgtat caaaaaaata
     6541 aatagaaagg aaggatcatt gtgcaggata cagtaaaaag gaaaatcgaa ttagaaaaaa
     6601 agcaacttag tttgaaaaac atgtatttca atcgttatct aatcgtacgt tacctgactg
     6661 cctttttctt tttcatgaac atgcaatgga tgattctgct tctatcagct aaaagtttag
     6721 gaagtagttt accgatgatt ctgattttag ctattttgcc tgcagtagga gaacaagtaa
     6781 agctttatcg aaaacaccaa acgaacgttc catggacaaa gtggtatttt ttgttccaga
     6841 gtgtctttaa tttattagtg attccagtgt tgtttacctc aggatttaca ttcttgtatc
     6901 cttttatggc aaataacaat ggaggacagt tatttgtttt cattcttgtt gtaagcggta
     6961 tttttgccag tgcatttata cagtatcgtt taaagaaaat ttcactcaac caagaccaac
     7021 agtatatccg tattaaacaa tatgaaaaag cgttatattt aggaaaggag aataactaat
     7081 gtttgatttt ttgcagaaat acctgatggg acctatgggg aaaattgcgc aacttaaaat
     7141 cgttcgggct gtcatggcag cggggatggc ttctattcct ttcacgattg taggctcgat
     7201 gtttttggtt ttaaatattt tgccattacc gttcccattt ttagaaggat tttttaatgc
     7261 aacttttttc aaagttagtg acttgtacat gatcgtcaat acaatgacga tggggatttt
     7321 atctatttac tttgccattg tgtttgctta tgaattaacg tcaattgaac gagatgaaca
     7381 aaaattaaat gttaatccgc tcacgggggc attactatct gtttttgcat tctttatgtg
     7441 tatccctgaa ttgatcatga ctgatggtaa aatcagcttg attgcttcta tcaatgagac
     7501 agaaactgta gtaaatggta ttaggatggg ctcttttgtc gaacgtttag gaacttcagg
     7561 aatctttaca gctattatta tgtcgattat tgcggtcgaa ttgtattgta tgtgtgtcaa
     7621 gagaaattgg gtcataaaaa tgccagatgt tgttccgcca ggagtttctc gttctttcac
     7681 tgctctgatc ccaacatttg tcattgcgtt tgtggtgatg atcatcaatg gattattagt
     7741 tgcactagga acggatatct tcaaaatgat tgctatccca tttgggtttg ttacgcagtt
     7801 gacgaataca tggcttggtg tcatggtgat ttatttcttg atccatgcat tgtggatcgt
     7861 agggatccat ggagcgaata tcatcacttc tttcttgacc ccgatcgtat tagctaatat
     7921 ggcagctaac gcacaaggcg caaattatcc gttggcagga gaattcaaca attcttatgt
     7981 aaccgttggt ggatcaggtg cgactttagg attgatcatc tttattgcat tcctcgcgaa
     8041 atctgatcag ctgaaagtgt taggaaaagc ctcacttgta ccaggattat tcaatatcaa
     8101 tgaaccaatc attttcggga tgccaatcgt ctacaatccc tatttagcgg ttccgttttt
     8161 cttagctccg atggcttctg ctacactcgc ttattttgcc attcggctgg aaatcgtcaa
     8221 accgatgctt gcacagatgc cgtggccttc accagtcggg atcggagcat ttgttggtag
     8281 tggtggtgat tggaaagctg ctgtgttagc agtcatttgt gcaatcatcg cattcattat
     8341 ctggttccca tttatcaaat tctatgataa taaattatta aatgaagaaa gagaaaaggc
     8401 agcagaatta gctagtgaaa agttaaaatg atttctaaga aaaaaggaac tgtgacaaaa
     8461 aatcacagtc cctttttttc ttagaaatgg gaaaccacta gtatataaat accattattc
     8521 ttcaatcaac cgcttcacga ttgcataatc cccttcataa ggttcatcta caccattgat
     8581 tttttgatag ggatccacgc cttttcccgc aataacgact gaatcttgcg gagcgctttc
     8641 tgctaaagcg cgtcgaatcg cttcaggacg atccatttca taatgaacga tcaaatctgg
     8701 attagtaatt gcttcattga tttcttccgc gatttttttc ggatcttcaa atgctgggtc
     8761 atctgccgtc aaaaaggcga tgtcagctgt ttctgatagc acttttccaa aatcatgtcg
     8821 acgggagatt gctttgtttc ctgggctgcc taatacaaca attacacggc catctggatg
     8881 ttcatttttg gcaaatttca gcaatgtttt caagcttaga taattatgtg catagtccac
     8941 atagacatgc gctccattgg attggatcaa ttgatccatt cggcctggta cacgagcttc
     9001 ttttaatccg tcttgtgcat cttgactgga ggcacctgtt agtgctgcgg caatgatcgc
     9061 actggctgca ttttcttggt tgaatcctcc agccagcaaa atatcataag cagtatcgca
     9121 gatagctaag cgatcttcat gagaagataa agtaaaaccg tgagtacctt tatctgaacg
     9181 gatcacttga taattgcttt cttccgaacg accataagtg atcgttggaa tctggaatag
     9241 atcggctgtt tccctaagta aatcaaaata gtcactttca tgatttaaaa caactgtttt
     9301 tgaattcaag atcaattgtc gcttgcaata gaaatagtca tcaaatgtcg gatgttcgat
     9361 cggactgata tggtcaggcg agatattcaa gaagattccg acatctaaag tcaagccgta
     9421 tacgcgttgt gttttgtaag cttgtgaaga aacttccatc actagatgag tcataccatt
     9481 ttcaactgct tctgacatca tacgatataa atctaatgac tcaggcgttg tcaaatggga
     9541 tttgaaatat gttttgccat ctaacgttgt attcatagta gaaagcaatg ctgttttttt
     9601 atttgttgtg tgatccaaga ttgcttttgt aaagtacgca gcagttgttt taccttttgt
     9661 tccagtgaat ccgatcactt tcaatttatt ttgcggatag tcgtaaaaag ccatagcaag
     9721 aagcgccatt gctttacgga tatccgttac gatgattcct actgctgaat tttcgtatgg
     9781 ttgttctgat acataatatt gaataccctg ttcgattgct gaatccaaat attcttcttt
     9841 gaaattcatt cctttacaga aaaacaaagt agaagcatca gcttttcttg aatcataact
     9901 taactgaaga aattcagctt tttctggtac atcaagatgc cagcctcttg ttgaaacaaa
     9961 ttcttttaat agattttctt ttaatagtaa atgacggatg tcttctaaag agaagctcat
    10021 tgattgatcc ctcgctttat tcaatttctt ggtttattat agcacactct ttttaatata
    10081 tcgtttgttt ccgctatgac aaaatccagc tttattcaaa ttttcttgac acaggggaca
    10141 agcggatgag tattttcata aacaatgaac tgactgtaat agataggaga acgaatagtg
    10201 tctccaacaa tccaaaaaca taataattca aaaagacgat aagactattg gtaagtaaaa
    10261 gtccttgcgg cattggaagc cgaaaaaatt tgtggaaaat aatcggcggt atcgttatac
    10321 cgccatttga ggccccgact gtgtacaatg tgtaaacccc aagactgaac aaaagacttc
    10381 ctgaaatcag tgaaaagaaa tgtgaggaga tcatttccac ttttggaaca atctctaaaa
    10441 caacaggaaa aagcagactt cccgctacag tcttaaaaaa tactgatttt cctaatagga
    10501 cagcggctag aagcagcatc ataagattga tgctccaaac agtaactgag cgttggatag
    10561 aaaaggccgt ttcaactaaa tgtccgatgg cgcccactcc ggcggaagct atatcgtgag
    10621 ggagcaagaa ccaattcaat ccaactgtga taaagaaaag acctaaaaat ataacgggta
    10681 gtttttttat ttcttgtgtc attgatatct cctataaatg ttagccataa aaggctgtat
    10741 atttctttac tttaccaaaa aaacgaaacc gaattcaaaa aaacaattgt tcttataaaa
    10801 gttatataaa atcgattgcc agtttctgtt ttaccaatta aaatgatagt aatgttttgt
    10861 agaaagttgg aatctgaccg atggataatc aaagaaaacc agcattagat caattaacac
    10921 cgcaagaaaa aatggcacga ggctctgctt ggatgaccgc cagtaatatc atttcaagat
    10981 tgctaggcgc tgtctatatc attccctggt atgcttggat gggcgaaaat gctaaggctg
    11041 ccaatggatt attcaatatg ggatacaata tatatgcatt gtttctactg atctctacag
    11101 caggaatacc tgctgcgatt gcaaaacaaa ctgcacgcta taattcattg aatgaatatg
    11161 gcacttcacg acgattattt atccgtgcat tacagatgat gggcgggtta ggcctgttgt
    11221 ttgctttgtt catgtacatc gcctctcctt ggctagctca tgcttctggt ggcggagaag
    11281 aactgattcc aacgatgcgt tctttgagta tcgctgtatt ggtttttcct tgtatgagtg
    11341 tgatacgagg gtatttgcaa ggcaatcaag aaatgatgcc ttatgcgctg tcgcagattg
    11401 tagaacaggt ggcacgtatt ttttatatgc tattggctac attcatcatc atgaaagtca
    11461 tggatggtga ctatgtgacg gcggtcacgc aatcaacttt cgcggcattt atcggtatgc
    11521 tagcaagtat tcttgtcctt ttgtattttt tgaagaaaca tcaagcgtat acatcggcgt
    11581 ttattcacta cagcgaaaac aaaataaata ttgcaacgaa ggaattgttg ttagataccg
    11641 ttaaagaagc gattccgttt attattgtgg gttcaggggt aacgatcttc aaattagtcg
    11701 atcagttcac ttttatgaag atcatgtctg catccaccga gtattccaat gcacagttac
    11761 tggatctttt ttcgattttt agtgccaatc cagataagtt gacgatggta gttattgcat
    11821 tggcaacatc gatcgcgtct actgggttac cgttgattac agaagctgtt acgttgaaag
    11881 atcgagtagg tctggcaaaa ttgacaagcg gaaatttaca gttgttttca ttttttatgt
    11941 ttccggcgac atttggtgtt atgttacttg cctatccgct aaatacatta ttttatacac
    12001 cggattctct aggaagtaat gtactgatcc aagcaagttt tgtaggactc ttcctaggac
    12061 tatatatgct agtttccaac atgctgcaag gaatgtttga aaacaaggcg gcaattaatt
    12121 atttggttat tgggtttcta gtgaaactcc ttcttcaata cccagccatt cgtttatttg
    12181 aagtatacgg ccctttattg gcgacaatga tcggatttgc tgtgtcttgt accttgatct
    12241 taaagagaat ccatgaagta gcccgcttta aaccaagttt tgtctggcgc aggacattgc
    12301 tgatctttat tcttaccttg attatgcttt tggcttcgtg gatcacaaag atgatttttg
    12361 gtgctttttt aaacgaagat agcaaattcc agtctctatt gttgattctt gtagtagcag
    12421 gtataggggc tttagtgtat ggttatttag ctttgaaaat ccgcttggca gaaagactac
    12481 taggaccagg aatagcgcgt ttacgacata aattaaaaat aaaataagtg aatcagaaga
    12541 gctagattga tgctcttttg aaatggggga aacgcatgcg tttagataag tttttagcag
    12601 atatgggact tggcagccgc aaagaagtca aaggattaat caaaaaagga acgatcgaag
    12661 tcaatggaca agtggtgaaa agcgataaag cgcaagtaaa tgaattcgaa gatgcggtta
    12721 cttatttagg agaaatcatt acgtatcaaa aagattttta ctacattttg cataaacctg
    12781 caggagtcat ttctgctact caggataatc atgatcaaac cgtaatcgat ttattggaag
    12841 accaggatta tcgggaagat ctttttccag tcggccgatt agataaagac acagagggcc
    12901 tcttgatttt aacgaatgac ggggtgtttg cccatcagtt actatcacct aaaaaacatg
    12961 tggagaaaga atatctagct gaggttagcg gtataatgac agaagaagat atccgtttat
    13021 ttgcagaagg actgattatt gatggcgatg agcagacatt gccggcaaaa ctccaaattg
    13081 atcaagtaga tcaagagaca gaaacttcaa ttatccgtct gattttgcat gaaggaaagt
    13141 ttcaccaagt taagcgaatg gtcaaagcag taggaaaaga agtcttatat ttaaaaagaa
    13201 tccgtatggg aaaattttgg ctgcctaaag atttgaataa agggcaatat cgacccatga
    13261 ccaaagaaga gctagaacaa gtgagagaaa aataattgaa tggatagcta aaagaataag
    13321 cagagtagtt gtagcgccat ctgaaaagca gacttgtccg tctgactttc ggatggcttt
    13381 tttgtgaaat ttcggcttat ttccgtagaa gctacttctg gaacaccgtt tgttcggaat
    13441 aagggactgt gacaagactt ttgttacagt ccccattgtt ccttgtgact cagagttaga
    13501 tagtaaggac acgatctccc taatactcag gccaaaacgc tgaattcaca acctctgata
    13561 aacggtgttc aatcagctga ccctcggtat tagctcaaaa aacggcaaaa tatgaggaac
    13621 tattttcccg tttttttctc taatactcag gccaaaacgc tgatttcaca acctctccta
    13681 tactttgttt cattttccct atactttgtt tatttattct cattattgaa agcgctataa
    13741 ttacgttgcg aatgaatcga aaggggtttt tagatgaaaa aacgaaaggg gttcaatcat
    13801 ttttttatga atctatggcg ttataaagca cttgtcctga tggctgtacc gggtatgctt
    13861 tggatgattt ttttctttta catacctgta atggcgaatg tcgtagcatt caaaaatttt
    13921 catatttcag cagatgggtt cttggctagc ttgagggaaa gtccttgggt gggttgggaa
    13981 aatttcaaat ttttattttc ttcggatcaa gcatttctca tcactaaaaa tacaatttta
    14041 tataatgtag catttatcct tttgaatctg ctgatctcag tcgtatttgc aatcattatg
    14101 agcgagttga gaaataaacg actcgttaaa gtttatcaaa cgatgtcctt actaccttat
    14161 tttctatctt gggtcattat cagttacttt gtttacgctt ttttaagtcc tgacaaagga
    14221 atattcaatc agtggatcac tggacatgga ggagaagctg tcaattggta taatgaaccg
    14281 aaatattggc cgtttatctt ggtgttcatt ggtacatgga aagggattgg ttataacagc
    14341 attatttact ttgcatctgt tatgggaatc gatccgactt attatgaagc tgctatggtt
    14401 gatggagcta gcaaatggca gcagatcaaa catgtgacga taccacaact tattccacta
    14461 atgacgattt taacgatttt agccgtagga aacattttcc gagctgattt tggcctgttt
    14521 tataacattc ctaggaattc cggtgcatta tatgatgtga ctcaagtttt ggatacgtat
    14581 atctacaatg gcctgacatc cacaggagat tttggtatga cggctgctgc aggactttac
    14641 caatcagttg tcggctttgt cttgttgatg atcacaaata cgatcgcccg acgttttgat
    14701 agcgattctg cattattcta aggaggaaaa aaatgaaaaa aaagaaagtg gagagagtcc
    14761 aagtccgctc tttcaataag acggctaatt ttttctctaa tataatgatt gcactctttg
    14821 ctatttcctg tattttgcca tttatatttg ttatcgccat ctcttttacc gatgagtcca
    14881 tattgtcgac tcacgggtac agtttttggc cagaaaagtt cagtatattt ggttatacgt
    14941 acttgttcga ccagatgcag gataaaattt tccaagcgct tttcgtcact gtacttgtta
    15001 ctgttttagg tacactgatc aatagcacgg ctacttcttt atatgcttat gcgatttcac
    15061 ggtcaaactt tccattccgt cgttttttta ccgttttttg tttgattacg atgttgttct
    15121 caccagggat ggtggccaat tatcttgtta tgacaaatct tcttcagtta aaagatacga
    15181 tttgggcatt gatcttgcca atggctgtca gtcccttcaa tattattgtg atgcgcacat
    15241 tctttaaacg ttcggtctct gattcgatta ttgaatcggc acggatcgat ggagctagcg
    15301 aactgcgaat atttgttcag atcgttttac cgttagctgt tccaggtatt gcgacaatca
    15361 gtttatttgc cgcattgggt tactggaatg actggttcaa tgctttacta tacatccaag
    15421 atgataagct agttccacta cagtatcttt tgatgaaaat ccaaagtaat attcaatatc
    15481 tgacacaaaa ttcaggagca ggaagccaat tgacaggcgg attagcctca gttccaggag
    15541 aatctgccag aatggcgatc gtagtcatct cgacgttgcc tattgcgatc agctatccat
    15601 ttttccagaa gcattttgtc aaaggtctaa caatcggcgg agtaaaagaa taaagaaaca
    15661 aaaatataaa tcataaaatt aggaggaaga aaaatgaagt tatggaagaa actagccttt
    15721 acaggtgttt cagcgttgtt gttgggaaca ttagcagcat gcggaggttc tggttctaaa
    15781 gaccaggcag aagctagcaa ttcggacact ttgcagatgt atcaaatcgg agataaacca
    15841 gataatttcg atcaattgat ggaagtagca aacaaacgaa tcgaaaaaga gatcggtgta
    15901 aaagtcaata tcaattacat tggctggggc gattacgaaa agaaaatgaa cgtcatcatt
    15961 tcttccgggg aaaattatga tatcgccttt gcgaacaact atgtttccaa tgcccaaaaa
    16021 ggagcctttg ctgatttgac tgaattagcg cctaagtacg ctgaaaaagc ttataatgat
    16081 ttagatgaag catatattaa aggaaatctg gtcaatggaa aattatatgc tttcccagtc
    16141 aatggaaatg tctttgctca acaagtactt acttttaaca aagctttact agataaatac
    16201 gatctttcaa ttgatggaat ccaatcttac gctgatgcag aaaaagtctt gaaggaattc
    16261 catgaaaaag aaccaaacac ggcagctttt gccattggac aaggcttcaa agtccaaggg
    16321 aactttgatt atcctttagg gaatacgtta ccatttgcta ttgatctgga tggagatgat
    16381 tcgaaaatca tcaaccaata cgataatgaa acgtatatcg gactgttacg tacaatgcat
    16441 agctggtaca aacaaggact cattccaagt gacgctgcaa cgagcaataa ggattatcca
    16501 ctagaaggaa atacttggct aatgcgtgga gaaacacaag gaccgtatga ttatggcgat
    16561 acaattttga caaatgcagc aaaacaagaa cttgtctcaa aagcaattac tgttccacta
    16621 aaatctacag ctcaagcaca aatggcaaat tttgtcgtat caaatacttc aaaaaataaa
    16681 gaaaaatcag tagaacttct agggctgctt aacagtgatc cagaattatt gaatgggctt
    16741 gtttggggga tcgagggaga agcatgggaa aaagtcgatg gttcagatga taaaatcaaa
    16801 ttgctagatg gttatcagcc aaatacacat atgtctgctt ggaatacagg aaacaacaag
    16861 attctatata cacaagagtc tattacggat gacatgattg ccaaacgtga tcaatcgatt
    16921 gctgatgcag aaacttcacc gattttagga ttcagcttca acactgacag tgtcaaaaca
    16981 gaattaagta atatttctaa tgtcatgaat caatatttag atggattgaa tacagggact
    17041 gttgatccag atgaaacatt accgaaattg aaagacgctt tggacaaggc tggctatgat
    17101 aaggtattga aagaaatgca aaaacaatat gatgagttcc gacaagaata ataatgaata
    17161 aagacaaagg ggttgtgaca aaagacgtgt cacaacctct tgttccgaat aaacggtgtt
    17221 caaaagccag tttctcggta ttaagctaaa tatatgtaaa aatgtggaaa gccatattca
    17281 caaaattttt cttactactc gaaactaagc ggctatttca caaactcttg gtaaacggtg
    17341 ttcaatcagc taaccctcgg tattcgctca caaaacgaca aaatatgagg aactattttt
    17401 ccgtttgttt ctctaataat caggtcaagg tgctgattgc ccaacctttt tttgtataat
    17461 gagaaagtat aaacttaatg tttggaggag aagatatgtt agggaaggtt ttggaagagt
    17521 tatttatccg gatctgggtc attataaaac taaccttgta tttctggata tatacatttg
    17581 ccggcgggat catctttgga ttaggtgcgg catggaaaac agtcaatgaa ctattttatt
    17641 tgtacggatt cgagtataaa gaaatcacaa taaaaagagg ctggaatata tataaaagaa
    17701 attttttaag aggcaatctg ctatttagtt tatttttatc tggtactgct ttattgagtt
    17761 acaatttata tttatctgtt caaatcaaag ggctcatttt tttagctatt gattttattt
    17821 tactttttgc cttgctctgc ctatttgcta cttatcaata cagtatgatt ttagatagtg
    17881 aatatacgat ctctgttcct aatctgctaa agctaagttt tatttcggtt ttttccagct
    17941 tttcgtcttt tctaaaaata attattggta gcggtataat tttaggcgtg acttggcaat
    18001 tcaaaggatt gatcctgttt ggtgtcattg gtcttttgac ggtttggaat ggtacgatga
    18061 caacacattg gcgtgaagaa ttagataaac agttggaaag ttatgaataa gtttcggatt
    18121 ctattttcaa aaggaacctt actaaatcgt ctgttgcgga aatacgcggt tatcatgatc
    18181 ggtgtaacga tgactgctac cgttattttt agtatccata cttgggaaca aaatcaaaaa
    18241 caggcagaaa acatgacaag tgatgccgtt caaagcacaa gcagaatgct gaatgataaa
    18301 acaaccttga gtaggatcat caaaaaccag cttgtaggga attccgaaaa gatcgaaaat
    18361 gtgacgacct acttaacaaa acccatagat cagtatctga tgtatgtata cgaacagcaa
    18421 aacagtaccg atgaattagt ttctttccca aatcaaatca aagatctcta cgcaaattat
    18481 gaagaactaa gtgcgatata tatcgtgttg aatcaactac cggagtatta tgaatcttct
    18541 agagaaaaca aaggtgggga aataaaaaag gggacaccaa aattactaga cgccttttat
    18601 atcaagttgc ctatcacaca aggaggagct gagtcaggaa gtatttttat tggttttcaa
    18661 aagaaagaac tagatatgat tttagaaaat ctaacgtcat tcaatggact ctctttatac
    18721 atgatttctg gaacgacaaa tcgactatac acattccatg ataataaaat agctaaagaa
    18781 acgtttgaaa gacaagaaat gatcatcaca gagagtctga agaaaaattc acaattgccg
    18841 attgacgatc tggaaaaaaa caattttgtt caacaccaag aactgtctga tgattatcgt
    18901 atattagctg tactagataa aaaaagtgta aaaatcgaaa cgggaaaaaa tcttgccccg
    18961 ctgatcattg gtatcattat tttagacaat atactgctta tctttcttta taaaacattc
    19021 aaacgatatt cacagcaagt atctatcgtc atgcaggcca tggatcaaac agcagcaggg
    19081 aatttagaga cgcggatcga cataacaaac acggaatatg aactgaggga actttcaatc
    19141 ggtatcaacg aaatgttgga tagcatcaat cagtttgtag aagacatcta caaattagaa
    19201 atcaaacagc aagacgcaca tatgcgggcg cttcaagcac agatcaatcc acacttttta
    19261 tataatacat tagaatatat ccgaatgtat gcaatcagcg aaggaagtga agaattagca
    19321 gatgttgtgt atgcgttttc cgctttgctt cgaaacaata cgaatcaaga gaaaacgatc
    19381 actttaaaag aagaactgga cttttgcgaa aaatatgtct atttgtatca aatgcgttat
    19441 cctaatcgag tagcctatca ttttatgatc gatcctgatt tggaaaagat cgaagtacct
    19501 aaatttgtga tccagccatt agttgaaaat tattttaaac acggtattga ttttactcga
    19561 ttcgataatg cgctaagtgt caaagtcttg caggaaggca aacgggtaag gatcatcatc
    19621 aaagataatg gaaaagggat gactgaaaaa cggctaaaac agattgaaga aaaactgtct
    19681 catcctaaag tggaacttca cggttctatc ggtctgcaaa acgtgaatga acgtttacgt
    19741 gccagcttcg gcagcagtta ttatatgtcg cttgagaata atgagacagg tggactgacc
    19801 gtttctatta cattcaagga gggatgagcg tgtatcaagt gttattggta gatgatgaat
    19861 acatgatttt gaatgggtta aaaaagataa ttgattggca aagtttaggc tttcaaatag
    19921 tagcaacagc agagaatgcc aaagaaggac tagctgtact tgaacagcga cagattgatt
    19981 tagtagtaac ggatgtgaca atgccagaaa taaatggact ggaatttatt gaagcggctc
    20041 aaaaagaacg tcataatttt gaatttatga ttttatcagg ataccaagaa ttcgattatt
    20101 tgaaaggagg tatgcaatta ggcgccgtga attatttgat gaaacccgtg aataagttcg
    20161 aattggttga aagcctcaag aaaatcaaaa cacggttaga tcagcaaaat gaacaaaaaa
    20221 atcaacaaga aatctatcaa gaactgttat tcagtcaatg gttgaatgaa gaattggatg
    20281 aaataagtga agaagaaata ataggacaaa taagtgagaa gcagcatagg atagtattga
    20341 ttcaactggc aagaactcat gatactctta tcaatagatg gttgcaagaa cggcaacaaa
    20401 tcttttacta tcaacgaaat tacggagacc gtgtattgat cacgttgttg ttggaggcag
    20461 atgaagttga tggcttctgt tgttttgttc aagaaaactt tcctgatcag gaatggctga
    20521 tcagtatcgg ggaagagaca gattctttgg acaaaatccc tgaaacattc caacaagcga
    20581 aagacaatct acagttgcat caattttatg gagataaaga cgagcatgtt ttttacgcag
    20641 tacaagcaga aacaaaagat caaaccatcg atttttcaag tttcagacgc gtgctgcaaa
    20701 ataaacaatt ggaagtcgct caaaaaatga tcactgattt ttttgaacag tttcagttag
    20761 cagccatgat gccggaagat atacgatatt ccagtttttt actgtttatg gagattcaac
    20821 gggaattgat tgatctggaa gatgaggaat atttacaagg aatcgaaaaa atccatcagg
    20881 caaaaacggt gcaagaactt cagcagctac ttctgtcatt catcaaaaag catcagagac
    20941 agaaaaagta cagtgacaat gtggaaaaag taatcgtaat cctgcaccag cattatcaag
    21001 aaccacttac actcaaagaa gtgtcagaaa acctgcattt aaatgtcatg tatctaggac
    21061 agctgttcaa aaaagaaacg aaaaaaagtt tttcagctta tttgaaccat ttacgtatgg
    21121 aaaaagcgaa acaactgctt ttgcacagta atcaaaacat caatgagata gcaagtgaga
    21181 ttggctacaa caacaccacg tatttctcta aattattcaa gaaaatcgtc ggcagatccc
    21241 ctaaagagta ccgagaaaac agtggagaat cataaaagaa aaagccaatg taatgaaata
    21301 acttataaaa aattgtctag aaatctaatt ttgggctgtt cttattactt gaggcaaggc
    21361 agctttttca tgacctctcg ataaacggag ttcaaaagcc agtttctcgg tattaagcca
    21421 aatttatgca aaaatgtgga aagccatttt cacaaaattt ttcttaatac tcgaaactaa
    21481 acagcttttt cacgacctct ccactaaacg gtgttcaatc agctgaccct cggtattagc
    21541 tcaaaaaatg gcaaaatatg aggaactatt ttgccatttg tctctctaat actcaggtca
    21601 gaacgctgat ttcacaacct ctcaataaac ggtgttcaaa agcttgcatc tgcggtaata
    21661 agaccaacca aacgaaaaat atgaggatct atttttgctt ggctgttctt attacttgat
    21721 gcaggacagc tttttcacaa cctcttgtta attatagttt gataaggaaa aaggcaaatc
    21781 ttttgcttga tatttcttag ggtgagggat gatccctaat tggtaagaga tcgtgccgcc
    21841 tttcagcaaa tcttgatggg taaaatataa tttcgtatat tctctgtcca tccgtttgat
    21901 tttatggata aattgttgct gcggccgatt aggcgttgct tcaatcgtca attcctttcc
    21961 atttgacaaa agcaaaacag ccttgtcaat taatggcata ccgataacat attcaccggt
    22021 tccaggcgtg acaggataaa atcccagact attgaagata taccaagcgg ccatacttcc
    22081 attgtcctca tctccagggt aacccattgg agaatcgtcg aaggtctgag tcatcaactg
    22141 tttaagcagt ggctgtgcca tttcaggttg tcctatataa ctgaacaagt aaggatagtg
    22201 aaaacttggc tggtttgaaa tagctaattg tccaaattct atcgcggcca tctcactcat
    22261 ttcgtggatc tcaaaaccat atcctcctgg gtcaaacgtt gggggctgat tgcacagctc
    22321 aatgagtttt tctttatatc tctcactgcc accatatttt ccgatcagtc ctgaaaaatc
    22381 gtgataaaca gcaaagctgc tttgccaagc acttccttct gcataatctt gtccccaacg
    22441 attagctgaa aatggttctc tgaacttgcc atctttatct tttgaacgca taaatcctga
    22501 ctcatagtca aagatatttt gatagttcaa tgattgctgc tgatatttct tcgtcatttc
    22561 ctcttcttct agtaccttgg caacttggct aatacaaaaa tcgctataag catagtctaa
    22621 tgtgtgattg actgattcgt ggtagtgatt aggaacatag ccatatgtca agtaatcttc
    22681 tgttccttgg cgtccataat ttggatgttt gctttgaacc gtagcacctt tttccattgc
    22741 cgttagaaat tcaggcatca agtctcgtcg gatatctttt acagcagcat ctgcaatgac
    22801 cgcatcgatc aatgtcccag gcataagtcc acgttcatca ggagacagcc attttggcaa
    22861 atagccactt tctctatagc tgttcaaaaa accttccagc atttcttcgt atttttctac
    22921 agcaatcaaa gaataaagag gataaaccgt tttatacgta tcccaaaacc cattatttgt
    22981 atagagaaca ccaggtttta tagatcgact agtcgtatca tagtggattt tttgcccact
    23041 aggatcaatt tcataaaatg tttgtggaaa taaaaacacc cgatataaat tatgataaaa
    23101 cgtccgcaat tgttgttgat catggtgtgt gagctgtata cgatcaaaat aggactgcca
    23161 ttgttcttga ctgtgagcaa ggtaagtctc tttgctttgg ttttgttcta gttccaggtt
    23221 ataagctgct tgttccgagc tgataaaaga tgtcccaaat cggatagttt gtttttttgt
    23281 ctgttcaaaa tacaacggga tcgagtcgct atttttgttg gaaacagcta actcatcaat
    23341 tgttaaagga tattcaaatc tcaatataaa ataaaaagaa aaatcaggat cttctgaacc
    23401 agcaaaatgt gtgatttttc cttccactgt gtaaggatct ataacaaaga gctgatggcg
    23461 tcccggaaag gataaaagta aaccgctgtc atttttgctg taatcaatag ataagattcc
    23521 gccatacatg ctaggtatca atgtagaaca gatgccataa cgtaattgac agatcgacag
    23581 atgagtcggg caaaatatac tttcttctgg gcggtaagaa ctttgagcat ggaaaagggt
    23641 gttctcctgt agttttccat tgatcggcgt aaacagcata tgactgaaat cacccatcca
    23701 aggacttggt tgatgggtaa gacggtagcc ttgaaaaacg cggtcatcag gatgaaacca
    23761 ccagctgccg ttttgatccg tagtttgcgg tgcaaaataa ttcatcccaa aaggaacgcc
    23821 agtataaggc tggcagttcc cattagagaa atttggctga ttcgctgttg catgtctagt
    23881 atctatttga ccgatattca ttttatttct cctttgttga ttgtcttttg atgaatgaaa
    23941 ctggaaattt actatcggat ggtcgatctt ttgtctctaa ccaagtgacc aaagcatctc
    24001 ccgcaagctt acctaactgt tcaaaatctt gtgcgatggt tgtcagtggc gggtcaacaa
    24061 gagctgctgc ttggatatca tcaaatccga taattgaaaa atcttcaggg atatgataac
    24121 catgctgtct caagagattc atggtctgga tagcgacaac atcgttttca caaacaaaag
    24181 cagttacttc atacttagtg atataagaaa gcaattcatc taatgtcgcg cttttgtcat
    24241 ccagtgaagt gtggaaagtc aaaccagctt tgtccaatgc ttcaaggtaa ccaaggtaac
    24301 gctgtcgaac ggattgtgga tgagtctgcg taccaaacaa ataagcgatt tttgaatgtc
    24361 cctgttcgat caaataagag gcagcaagcg caccgccttg gaaattatcg gaaagaacgg
    24421 taggaaaagg taactcatag atttttttat ctaatatgac gacagggaat tctttcaatg
    24481 aaagctcaaa aagtaagtca agatgttgtt ctgtatcaag agcataataa atcaatccgt
    24541 caaattcatt gattagctct tttgctgttt tattttgtag aaagtcaagc gtcgtcatca
    24601 tgacatcaat ctgtttatct tgcatcacag ggttcaatcc cttgctaaaa tcaccaacag
    24661 acaaatcagc gacatatgga agaagaaaca gaatccgtcg tgtttttgta ggagaagcaa
    24721 gaatctgttc cggagaagcg gatttaacga aactgccttt cccgcgaacg cgataaataa
    24781 gtcctaattg ctctaattca gtcaatgcac gtttggaagt gattctgctt actttatagg
    24841 tacgtgaaag ttctttttca gttggtagtt ggctgtctac aggtaattca ccagttaaaa
    24901 tagccgtttg caaatcatta aatattgtct ggtacaatgg ttgagtcata taaacacctc
    24961 ttaatgatat atcatattca aataatacca taatggatga aaaagtaaat aaaaagatat
    25021 tttttgaaag cgtagacata attggctgta tgaagtataa tctttaaaga tatatcaaat
    25081 agaaagaagg ttttattagt ggtttataaa gatataccaa aatcggtgca gaaatttatg
    25141 gaagatatca cacaaaaatg tggggatatc catgcggact gggcaaagaa ttttacagca
    25201 gcatttgcca atactctgtt gacaacagtc aaaaagcaaa atgacggaac caccttttta
    25261 ttgacgggag acattccagc gatgtggtta agagactcta ctgcacaagt tcgtccttat
    25321 ttagtgattg caaaagaaga tgaggaatta gctgacatga tcagcgggtt agtcaaaaag
    25381 caattttttt atatcaatat cgatccatat gcgaatgcct tcaacgaaga agctaacggt
    25441 gctggccacc aaacagatca tacaaaaatg aacgattgga tctgggaaag aaaatatgaa
    25501 attgattctt tatgctaccc aatacagttg gcctatcttc tttacaaaaa tacaggaaaa
    25561 actgatcaat tcaatcaatc ttttattgaa ggtatcaaaa aaatcttgga cgtgttcaaa
    25621 acagagcaag atcatgaaca ctctccttat acatttgtac gagacacgac aagaaaagaa
    25681 gatacgctga tcaatgacgg gcgaggtgca aaaacagctt ttacaggaat gacatggtct
    25741 ggtttccgtc ctagcgatga tgcttgccgt tatggatatc tggtaccatc caatatgttt
    25801 gcggtggtcg tgttaggtta tgtggaagaa atttttcaaa cgatactcga tgataaagaa
    25861 attgtacagc aaacacagaa attgaagaaa gagatccaag aggggattca gaatcacgga
    25921 cgaacggtta atcaaaaagg gcaaacgatt tatgcatatg aagtagatgg aatgggaaac
    25981 gccacagtca tggatgacag taatgtgccc aatctgatcg ccgcacctta cttgggatat
    26041 tgttctgaac aagaggaaga ttatctgcaa acaagaagaa cattgttgag taaagaaaat
    26101 ccatatttct atgaaggaaa gtacgcaaaa ggaattggat cctcccatac gccagaaaaa
    26161 tacgtttggc cgattgcatt agctatggaa ggaatgacaa cgtcagacaa atctgaaaaa
    26221 gagcggatat tgaatttact tgtagcaact gacgcaggaa cacaccttat gcacgaagga
    26281 ttcgatgtga acaatcctga aaattatacg agagaatggt tttcttgggc aaatatgatg
    26341 ttttgcgaat tagtcatgga ttactttgat attagagtag aaaagtagga ggtatcgtaa
    26401 atgaaaaaga aagtatatat catttctcat agccactggg atcgtgaatg gtacatggct
    26461 tacgaacagc atcacatgcg actgattgaa ttgatagacg atctactaga gctatttgaa
    26521 gttgatccgt ccttcaacag ttttcatttg gatgggcaaa cgattatttt agatgactat
    26581 cttcaagtaa ggccagaaaa aagacaggca atccaacaag caatcaatga aggaaaattg
    26641 agaattggac cattttatat cttgcaagat gattttctta tcagctcgga atcgaacgtg
    26701 agaaatatgc tgattggcat ggaagaaagc cgtaaatggg gaacccctgt catgttaggc
    26761 tattttcccg atacatttgg aaatatggga cagacgcctc aattaatgaa acaagctggt
    26821 atttcagcag cagcgtttgg ccgtggtgta aaaccaattg gctttgataa tcaagttttg
    26881 gaagcagaaa actattcttc ccaatactca gagatgtggt ggaaaggccc tgatcaaaca
    26941 gcaatctttg gtctgctatt tgccaattgg tatagcaacg ggaatgagat tccagtagaa
    27001 aaagaagcag ctcttgcctt ttggaagcaa aaattagcgg atgcagaaca atatgcctct
    27061 acgaaccact tattgatgat gaatggtgtt gatcatcaac cggtacaaaa agatatatca
    27121 aaagcaatcc atctcgcaaa tgaactgttt ccagattacg agtttattca ttctaatttt
    27181 actgactatc tggaagcagt tcaaaaagat gtgccagaag acttaggctc agtagaagga
    27241 gaattgacaa gtcaagaaac ggatggctgg tatactttag ccaatacagc atctgctcgg
    27301 gtttacttaa aacaatggaa taccaaggtc cagcgacagc tagaaaatat tacagaacca
    27361 cttgcgacga tggcttatga agttagtgga aactatcccc atgatcaatt agattatgct
    27421 tggaagacat taatgcaaaa tcatcctcac gacagtattt gcggttgttc agtcgattct
    27481 gtccatcggg aaatgatacc tcgttttgaa aaagcagatg aagttggaaa gtatcttgca
    27541 caagattcct tagaacagct gaccgcagca atcgatacga ccggattccc aaaagacagc
    27601 tttccatttg tcattgtaaa tactgcagga atggataaaa ctggtgaagc agaaataacc
    27661 atcgaattag aaagaaaaag attcgctgaa ggaatacctg aacaactata tcaagagtta
    27721 gaaaatttgc ctaaacgtaa gtatcatgta gaaacaaaaa gtggggccac tataccagct
    27781 attttatctg aagaaactgt ccaatttggt tatgatttgc cgaaagaccg tttccgtgtg
    27841 ccatatatgg caagaatgat caaagtgact ttaccattag aaaacatgcc agcattttct
    27901 tgggaaacat tcgcgcttgt agaaggggaa gcgaaagcag aagaaaagga aacaatgatt
    27961 catcagagtg gacgaatcat cgaaaacggc cctctacatt tgacgattga aaaaaatggt
    28021 accattacaa tggaagatcg caaaaacaaa cgaaagttga ataatctgca tatttttgaa
    28081 gatatcggcg atataggaaa tgagtatatc tttaaacaac ctttctgtga tcagccaatc
    28141 ctatccagta acaaagaaaa ctctgaagta aaagtattag tagatacacc ggaaatagca
    28201 aaaataagta ttctacagga aatggaaatc cctgtttctg cagacgagcg tttagaaaaa
    28261 gaacagcaga tggttgtgga atttcgttat cgaaaagcag aacgatccaa agaaaaacga
    28321 atccttcaaa taaaaacaat catgacaatc agaaaagata gtaaaaaaat cgactttgaa
    28381 acgactctcg acaatcagat gaaagatcat cgcttacgtg tcttgtttcc tacaaagtta
    28441 catgtggaac atcacgaagc agatagtatt tttgaagtgg tgaagcgccc aaaccatgtg
    28501 tctaaaagtt gggaaaaccc cacgaatccg caacatcagc aagcatttgt caatattcat
    28561 gatgaagagt acggtgtaac agtcggaaac tttggtttga atgaatatga agtaacagaa
    28621 gacggacaaa tcgcagtgac gctacttcgc agtgtgggag aactaggcga ttggggatat
    28681 tttccaacac cagaagcaca gtgtctaggt gagcatcggt tcaattacag cattgaacta
    28741 catggaccag aagaaaaatt ctctacttat ttgcatgcat acgcagcaca gattcctttt
    28801 tccactcaac aaatcaaaca tcatgaagga acattgattt caaaacaaca atatttaacg
    28861 ataaaaagtg aaacgtttgc aattacagct ttgaaacgca gcaagtttag tgataaagta
    28921 gtagtaagag gtttcaatat gtctagtcat ttagaaaaat tggaaataac taaagacaat
    28981 ggaaaaactg tcatattgaa tctgcttgaa gaaccaacga agcaagcggt tgtcccaata
    29041 atccaacctt atgagattcg gacaatcgga ttcgaggagg aaaacgaatg tatggcggaa
    29101 tagaagcagg cggaacaaaa tttgtgtgtg cggtttcaga tggtggagaa attttagaaa
    29161 aattaagtat ccctaccaca acaccagaag aaacattgga tcaggtattt gaattttttg
    29221 atcgttatga attgaaggga atcgggattg gctcattcgg cccgattggt atcgacaaaa
    29281 caaaagataa ttacggatac gttttggcaa ctcccaaaaa agggtgggca caattcgatt
    29341 ttcttggcac aatcaaggaa cgctacaata ttcctgctgc ttggacaacg gatgtcaatg
    29401 ctgctgcata tggggaattg aagcaaggag cagcacaagg aaaaaatagt tgtatttacc
    29461 ttaccgtggg aacaggaatc ggtgcaggtg tcgtgatcaa tgaagaaatt ttttcaggga
    29521 tcgcacatcc tgaaatggga catatttggg tgaaacgcca tccagatgac cattatgaag
    29581 gtacttgtcc ttatcataaa gattgtttag aaggattagc agcaggaccg tctattgagg
    29641 cacgcacagg gatcaaaggg caagatctgc cgcaagatca tcctgtttgg gctattcagg
    29701 cttattatat tgctcaagca ctgatcaact atacgttgac gttagcacct gaaaagatta
    29761 ttttaggcgg tggggtaatg aatcaggatc atctccttca aaaaatccgt cagcaatttg
    29821 ttgaattgat gggtggttat atggaaacac ctcaagtaag tgaatatatc gttcgttggg
    29881 gactgccgaa tgaaagtggg attatcggta gtttgctgtt agcagaacac gaacatcaaa
    29941 agtaagcaga caaattacac aaaaagaaca gagtcttatc attatctgct gataagactc
    30001 tgttcttttt tatttctatt cccataatcc aggtaaagct ttaccgacta ttttttcttg
    30061 agtaaatgga tggatcatgt gcagttctaa tgcatggagc atcagtcgtt cagctgttct
    30121 attttggtag agagggtctc caacgatcgg atgtccctta tgagctaaat gaactctgat
    30181 ctggtgtgta cgtccggttt ccaaaacaca atagacttgt gtttgttcag aaagtacttt
    30241 gttgacagtg acatgtgtca aggcgctctg accattttta tcatctacga ccctttttct
    30301 ccgatcatgg cgatctcgtc caattttatt ggtgatggtc atatctttgg caatttctcc
    30361 ccatacgatg gcttgatatc tccgatagat cattttttgt tccaacattc ttccaagtat
    30421 cggcaagaca aatggatttt ttgcaaataa aacggcaccg cttgtttctt tgtccaagcg
    30481 gtggacaacg taaggtcgtt gctgtttagt gtcgagataa gcagctaaat gattgagtaa
    30541 ggtactgttt tctgtcggtt catttggatg agtcttcatt cctgcaggct tatccactat
    30601 aatcaaatgc tcatcttcat ataaaacacg gatgtgcttc gcatctccta attgaacttc
    30661 ttgataatta taatctgttt cttcgaataa caatgtgatc tggtcattgc cctttacttc
    30721 atgatggaac aaagcaggtt gttgattgat ccaaacgttt ttacgtatac gcaaaaagtg
    30781 tcgaaccttt ctaggaacta accattcctt ttcaagcaag tcgcgaattg ttgtttcagg
    30841 atggttcata ggtaaagtga tcgtgatttt cacaagtgta ctcctttcag tgcaattgca
    30901 gactaattgt accattttta aaacatattt aagaaaaaac ttacattttt ctgttcattc
    30961 ttatgttact ctacttgcac taacgtaaaa aaacatattg aactacaact taagcaggaa
    31021 gaaaacactc tttcactatg gtaaaataag acgattgaat caaatagaca ggagaagaag
    31081 caaatggatt ttcaaagcat tttagaaaag ataaaagctg ctttgatcag tttttggcgt
    31141 tggatcagac catatctcgg acaattccat cgatggcgaa agcgcatctg gaaaaaatat
    31201 catgtgaaca aattgatttt gctgattggt ttgatctgtg tcttagtgac aagtatctac
    31261 ctctttattt tagcaaaaca agcaaatgtc gaaacgttaa aatcaggatt aagccaatcg
    31321 acagtcattt atgataagaa aaacgaagaa gctggcacac tgtacgccca aaaaggaaca
    31381 tatgtagaat taaacgcgat ttctccctat atccaagatg cagtaatctc gacagaagat
    31441 aggaattttt acgagcatca cgggtttgat atcaaaggga ttgcccgtgc agcagtccga
    31501 atggtcttgt ctggcggaac atcagggggc ggaggaagta cgatcactca gcagcttgcc
    31561 aaaaatgcgt atctgacatt agaccaaacg tttgatcgaa aagcgaagga attatttttg
    31621 gcaatcgaga tcgaaaagaa gtacagcaaa gaagaaatct tgaccatgta tcttaacaat
    31681 gcctattttg gcaacggggt atggggtgtc caagacgctt ccagaaagta ttttggagta
    31741 gatgcaacga atgttacgct gtctgaggct gctactttgg caggaatgct gaaaggtcct
    31801 ggtatttata atccgatcga tcacccaaaa aatgcgaatg atcggaaaaa cacagtacta
    31861 agtgtaatgg aagaaaacgg aaaaatcaca aaagaacaag aacaagcaga aagccagaca
    31921 gatatcagcg cgtatcttaa tgacacatac gataattcag attctggtta tcgttatcct
    31981 tattattttg atgcagtgat cgatgaagca gttaacgagt atggtcttga tgaagaagat
    32041 gtcatgaata aaggctataa aatctataca gctttagatc aggattatca aaagcaatta
    32101 gaagctactt atcaaaacag tgcattattc ccagcaaatg cttcagatgg tgccatggtc
    32161 caatctgctt cagtagcttt agatccgaat acaggtggtg tacaagcact agtaggacga
    32221 agaggagatc atgtctttag aggattcagc tttgctacac agatgcgtcg ttcaccagga
    32281 tcaacgatca aaccattaag cgtatataca ccagcactgg aagctggata caagccttcc
    32341 agtatcttga aagacgaacc gcaaagttat tacgatgcgc ataattttga cgggacatac
    32401 caaggagaag taccgatgta tcaagctgtt gcacaaagtt tgaatttgcc tgctgtatgg
    32461 cttttgcatg agatcggttt acaaaaagga tatgataaag cagaagaatt cggcctgcct
    32521 ttagcaaaat ctgataagta ttatggctta gcccttggtg gactggaaaa aggagtatct
    32581 ccattgatca tggcgggtgc ttacagtgca tttgccaatg atggtcagat gtatacgccg
    32641 catcttatca cgaaaatcgt tgattcgact ggagcggtga tcgtcgataa cacaaacaaa
    32701 aaaccaaaac aggtcatatc aaaggaaaca gccgatgaaa tgaccagcat gcttcttggt
    32761 acattttcaa atggaacagc ggctgctgcg aatcctgcag gttatacgat agcagggaaa
    32821 acaggaacca cagaaactaa tttcgatgcc acaaaagcaa atgaccaatg gatgatcggt
    32881 tacacgccag acgtagtgat ttcgacatgg atgggttacg aagaatccag caagctgcac
    32941 tatttggaag ggacaagtgg aaatgttgtc gggaaagtct tcaaatcagc ggcagaaggc
    33001 atccttccgt atacttcaaa aacaggattt agtgtagcag atgcctatgc cacaggagga
    33061 caagtggtag ctgcagatca agttggaaac acaggaaatt ctaatggaga gtctggaaat
    33121 tggcaagata acttgaatca atatggtgaa caagcaaaag aagggctgaa aaacttcggc
    33181 gatatggtga aagaaggcgt tcagggaatt ggtgaagcgg ctaaagacct ctggcggaaa
    33241 tatcaaggag agtagcccct ttgaaaacga ataaccccat gttacaatag agagtaacta
    33301 ctaaaagaaa agagggcata acatgtcaaa catctacgat agcgcgaatc aaatcgaacg
    33361 tgagatccgt gaattaccag aattcaaagc gttgaaagaa gcgtacgaga aagtacaagc
    33421 aaacgaagaa gcacacaaac tgttcaaaga cttccaagcg atgcaaatcg aattacaaga
    33481 aaaacaaatg agtggacaag agttcagcga cgaagatgca atgaaagcac aagaaatggc
    33541 tgtgaaaatc caatcagaag aagtaatcaa tgacttgatg caaaaagagc aaggcttcag
    33601 tacaatcatc aatgacttga accgtatcat catgacacca gttcgcgacc tttacagcga
    33661 ataaaactat tttatgaaac aacagagggt gtgaaagaga cgttcggcct tgagcgatga
    33721 caaaattgct tcttattgcc gataggctgt ctcttgaaca ccatttacca agaggtcgtg
    33781 agcctaccgt ttaactctga gttacaagga acaatttcaa aaaacagctt ctcatgtttg
    33841 atgactattg tgacttgtga ccgaggagtt ggcaggagaa caccgtttat tcagaaatag
    33901 gagctacgac aaaagtcttg tcatagctcc tattttttgt cattcatttt tcaagcggta
    33961 gaaatagcct ttttgagcta acagtttttc gtaagtacct atttcaacga tttttccttc
    34021 atcgatcaca acgattttat ctgcctcttt gatcgttgaa agccgatgag ccacaataaa
    34081 agtagtgggt gtttctgtca ggtaattcag ggcttgctgg atttttttct ctgactggtt
    34141 atctaaagct gaggttgctt catctagcaa aatgattttt ggctgacgta tcaacgctcg
    34201 tgcaatggag atacattgct tttggccacc agacagcttg ttgccatgtt cgcctactaa
    34261 ggtatccaga ccatcaggaa gctggtcaat cagtgaagaa agttgcgcag cttcgatgac
    34321 ttcatctaaa cgttcttttg atacgttttt caagccataa gtgatgttct ctttgatcga
    34381 agcggagaac aacaaagtgg tttgaggaac aacggctaaa taatttcgca cacttcgcat
    34441 atccagttca tccaaaggct gatcatctag aaaaattttt cctgaagtcg gctggataaa
    34501 tccaatcaat aagttaagga gtgtagattt tccagagcca gatggcccaa caaaagcaat
    34561 tgtttctcca gctttcacct ctagagaaaa atgatcgata atcggtttga gtgagtccgg
    34621 ataaagaaaa gaaacatcct caaaacgaaa atttccatcc aaatgttgga tttttttctt
    34681 accgtgataa gtttctacat cgccagcaat caacagttcg ccaatagaag tgatagattc
    34741 catgcctttt gaaatcgtag gaatcagcgt gatcattcca gaaacggaat tgacgatcgt
    34801 cgtgaaatac gtctgataca tcacgacatc gccgggttgg atgcgccctt gtaaagctaa
    34861 tatcccagtg aaaatcaaac aaatcagctg aaatatctga aaactagccc agctgactga
    34921 tccgaaaaca gattgcagga tatctaagtg gaatcctttt tcatagattt gagtaaaatg
    34981 cttatttaag cgagttgttt cgtgtttttc taacccatga gctcgagcaa tcggtgctaa
    35041 ttcgatcact tcgatcactt ttgcagaagt ctcttccatt tcttgcctga agtctgagtt
    35101 ggactgtctg atccttttac ggaaaaagag agtaacgaat gcggcgagag gaacagtcaa
    35161 taagaaaaac atgaaaacaa caggactttt ataaagcgtg atacctaaag ctacgaataa
    35221 attgacagcg atatttaatc cgctgacaaa tacttgagag gacaacgttt ggatagcttc
    35281 tacatcacgg atgatctttg actggattcg tccagattgc aattcttttt gataaggaat
    35341 agatagttct tggattttct tgataagggt cgctcttagt ccagcttcta cttgacggat
    35401 cgaagtgcta tggtatttta catgaaaata attcgtcaat atattttgac caatcagaca
    35461 agccatcaaa aaagcgttga aataaatggc ctgcaactga gaagaatctt tcgatgaagc
    35521 tgcattgatt acattggcaa tagcgatcgg cattaaccaa gatggaagat gtttgataat
    35581 aaaaaagagt cctgacatgg caaaacgcca ataatttccc tgataaagat gcagcaatgt
    35641 ctgaaaagca tttgcatgat atttttgata acttttctgt attcgttcct ccataatctt
    35701 ctccttaaca atcttggtaa ataaactgca agtgttctat tgcttcagga acataaatct
    35761 caggagtagc ttcaagagtg gcgtacatta gaggacgctc atatttgata taatgcagta
    35821 ccgggcggaa atctaaacag cccttgccaa caggtaccat gcggatcttt ccattttcca
    35881 tgatatagtc tttgagatga agcgctgtaa tataaccatg taaattgtcc aaggcacctc
    35941 ggatcacttt ttcctgatca gcatgatttt ctttatgcat caaattcgca caatctaaaa
    36001 tgatttttaa atttggtgaa gctacttcat cgattaaacg tttagctaac tgatagttat
    36061 agattggatg attgatccct gcttcaacag caacagttac gccaaaattt tctgcatgag
    36121 cgactaattc aatgatagaa tttctggcga tctgataagc ttcttcagta aaattttctt
    36181 ttgtgtaacc gacagagaca ctaccggttt ccgtagcaat catcttggct tgataggcag
    36241 agcacagagc aatttgctgt ttgaaagttg ccaatgcttg tgcacgaact tcttgatttt
    36301 tgctggatag gttgatatag catcctaaaa tggaaagttc gatattgttt ttttggaagt
    36361 attcaccaaa ataggcagca ctcccaagtg tcatgtcagt taatttaagt tgttcaggaa
    36421 atgctttttg tggtgcaaat tggatatggg acaagtgaag ctgtgatact ttttgaacga
    36481 tttcttcacg atttcgaaca tctaaatcgt gggcacggat gcctaaattt aatggaaaca
    36541 tctcaaactc ctttttgtaa ataattagca agaggcagac gggattcttt tatcaattca
    36601 acaattattc gggcaacttt atcagcgcct tctggactaa agtgggtatt gtcttttagt
    36661 ccttctgggt aatttttaca agtatcaggc ataagatgca ggtagaattt ttttgtctct
    36721 tcttttgaga aggtctggaa caattcttgt gatcttgaga acacatctaa aagaggaata
    36781 tggtgtttct cagcaaatgc acgcatcgct tttggatagt ctccaagtat atctggattc
    36841 aatgttccgt tttctaagta atctcgtcgc gtcacagatg tcagtaagac catttggacg
    36901 ttcgcttttt ttgcgcattc aaaaaatatc tgcagatttt ttaaatagct accgaaggga
    36961 agcgtacctc tttcttcttg gattttctgg tcgttatgtc caaattgtac aaataagaaa
    37021 tcattcggct gaaactgtga agctagacgg gctaaccttc cttcctggat aaatgatttg
    37081 gtgctgcgtc cattctttgc ttggttattg atctggatct cgggtgaaaa ataagcagag
    37141 attttttctc cccaacctgt ctcgggacgc ttgttttcat ctttttctgc cgcagtagaa
    37201 tcaccggcaa tatggatagt aggcatgtta tctcttcttt cataaaggtt tataggcaat
    37261 gaagcagaac agcaagagaa gaagacggag caa
//
LOCUS       DARBET010000022        34944 bp    DNA     linear   BCT 07-NOV-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4027
            SAMEA114569841-rid20112183.denovo.022, whole genome shotgun
            sequence.
ACCESSION   DARBET010000022 DARBET010000000
VERSION     DARBET010000022.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA114569841
            Sequence Read Archive: ERR12203666
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 34944)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 34944)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001972618.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001972618.1
            Genome Coverage       :: 132x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/07/2023 13:18:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,041
            CDSs (total)                      :: 2,975
            Genes (coding)                    :: 2,908
            CDSs (with protein)               :: 2,908
            Genes (RNA)                       :: 66
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 67
            CDSs (without protein)            :: 67
            Pseudo Genes (ambiguous residues) :: 0 of 67
            Pseudo Genes (frameshifted)       :: 26 of 67
            Pseudo Genes (incomplete)         :: 28 of 67
            Pseudo Genes (internal stop)      :: 20 of 67
            Pseudo Genes (multiple problems)  :: 6 of 67
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..34944
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA114569841-rid20112183.denovo.022"
                     /isolate="VRE4027"
                     /db_xref="taxon:1352"
                     /country="Denmark"
     gene            complement(59..187)
                     /locus_tag="SCJ86_001202"
     CDS             complement(59..187)
                     /locus_tag="SCJ86_001202"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S9"
                     /protein_id="HEG1168776.1"
                     /translation="MDQKEKMKELIKKKQGGGRSKQMETPKNDRKNMRKGPKIFNK"
     gene            complement(190..1563)
                     /gene="rlmD"
                     /locus_tag="SCJ86_001203"
     CDS             complement(190..1563)
                     /gene="rlmD"
                     /locus_tag="SCJ86_001203"
                     /EC_number="2.1.1.190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (uracil(1939)-C(5))-methyltransferase
                     RlmD"
                     /protein_id="HEG1168777.1"
                     /translation="MTEQQIVKKNERYTVEIVDLSYEGMGVAKIDGYPLFIENALPGE
                     EVEILVVKAGNKFGYGKVETFITQSPDRQPVENNALLRIGIAPLAHLKYEQQLVFKQK
                     QVEHVMAKIAKMPEVPVLPTVGMAEPFGYRNKAQIPVRRIDGQLATGFYKKNSHDLVP
                     IEDFYIQDPVIDQAIKVVRDILQRFQVRGYNEEKNEGQLRHIIIRRGHYSHEMMVVLV
                     TRKEKFFKSKEIAEAIHEELPEVVSVIQNINEEKTNVILGDKEKVLYGRDYIEDQLLG
                     KTYRISAKSFYQVNTQQAEYLYQTAIEFAELDKKDIVIDAYSGIGTIGLSLADRVDKV
                     YGMEVIPEAIEDAQFNALNNKIENVHYEVGKAEKVMKKWQEKGIKPSVIVVDPPRKGL
                     DHHFIESAIEMSPERIVYISCNPATFARDAKLFAEAGYAVKKVQPVDLFPQTHHIELV
                     ASFVKGE"
     gene            complement(1674..2729)
                     /locus_tag="SCJ86_001204"
     CDS             complement(1674..2729)
                     /locus_tag="SCJ86_001204"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317640.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diacylglycerol kinase"
                     /protein_id="HEG1168778.1"
                     /translation="MKKARVIYNPTSGKELLKKNLADILQALEEAGFEASAYATTPEP
                     DSAKNEACRVAELGFDLVVAAGGDGTINEVVNGIAPLEHRPKMAIIPAGTTNDYARAL
                     KIPRDNIKAAAEVIKKHQTVKMDIGLSDNSYFINIAAGGYLTELTYEVPSELKSIFGY
                     LAYLAKGAEMLPRVKPIKMRLKYDEGEYIGNASMFFLGLTNSVGGFEQIAPDAKLDDG
                     KFSLIIVKTANIFEILHIAALMLNGGRHVDDPRIIYTKTSSLFVETEDDINRPVMINL
                     DGEYGGEAPMHFQNLHQHIEFFANTDQIPDEAITGTDEEELEVVSKEFVKEVERLTDE
                     DIDGDGKIADETPNQKN"
     gene            complement(2739..4169)
                     /gene="gatB"
                     /locus_tag="SCJ86_001205"
     CDS             complement(2739..4169)
                     /gene="gatB"
                     /locus_tag="SCJ86_001205"
                     /EC_number="6.3.5.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735264.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Asp-tRNA(Asn)/Glu-tRNA(Gln) amidotransferase
                     subunit GatB"
                     /protein_id="HEG1168779.1"
                     /translation="MNFETVIGLEVHVELKTNSKIFSPAPAHFGAEPNSNTNVIDWGY
                     PGVLPVMNKAALEFGMKAALALNCEISKDTHFDRKNYFYPDNPKAYQISQFDQPIGHD
                     GWIEIEVEGKKKKIRIERVHLEEDAGKNMHGIGGYSYVDLNRQGTPLIEIVSEADMRS
                     PEEAYAYLEALRSIIQFTEVSDVKMEEGSMRCDANISLRPYGQEEFGTKAELKNLNSL
                     SFVKKGLAFEEKRQAKVLLSGGEIQQETRRFDETTNKTVLMRVKEGSSDYRYFPEPDI
                     PKFVIDDEWIQKVKASLPEMPASRRERYIREFGLPEYDAMVLTLTKEMSDFFEAVLEN
                     GADAKQASNWLMGEVSAYLNSEKLELAETKLTPENLAGMIRLIADGTISSKIAKKVFR
                     ELITNGGEAQEVVEKNGWVQLSDPNKLLPIINEILDNNQQSVDDFKNGKDRAVGFLVG
                     QIMKATKGQANPGVVNKLLKDELAKR"
     gene            complement(4166..5638)
                     /gene="gatA"
                     /locus_tag="SCJ86_001206"
     CDS             complement(4166..5638)
                     /gene="gatA"
                     /locus_tag="SCJ86_001206"
                     /EC_number="6.3.5.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631886.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Asp-tRNA(Asn)/Glu-tRNA(Gln) amidotransferase
                     subunit GatA"
                     /protein_id="HEG1168780.1"
                     /translation="MTELHNRSLEELHHMLVSKEITVQDLTKTTFERIKETEPEVEAF
                     ITLNEEKALEQAKALDEKGIDESNVLAGIPIGIKDNIVTKDILTTAASKILYNFEPIY
                     DATVMDKIYQADMIPVGKLNMDEFAMGGSTETSYFKKTKNAWDPTKVPGGSSGGSAAA
                     VAAGQIPVSLGSDTGGSIRQPAAFNGIVGMKPTYGRVSRFGLIAFASSLDQIGPMTRT
                     VKDNALALTAISGYDEKDGTSSGVSVPDFAEGLTGDIKGMKIALPKEYLGEGVAPGVK
                     EAVLKAAETFRSLGAVVEEVSLPHSKYGVAVYYIIASSEASSNLQRFDGIRYGYRSEN
                     VKSLDDVYVNSRSEGFGEEVKRRIMLGTFSLSAGYYDAYFKKAGQVRTLIKRDFEKVF
                     ADYDLIIGPSSPTVAFGIGENINDPITMYMSDILTIPVNLAGLPGMSVPAGLSEGLPV
                     GLQIIGNYFDEKTMYQAAYAFEQATEFHKQQPAILGGKDE"
     gene            complement(5638..5943)
                     /gene="gatC"
                     /locus_tag="SCJ86_001207"
     CDS             complement(5638..5943)
                     /gene="gatC"
                     /locus_tag="SCJ86_001207"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002378010.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Asp-tRNA(Asn)/Glu-tRNA(Gln) amidotransferase
                     subunit GatC"
                     /protein_id="HEG1168781.1"
                     /translation="MAISEEQVKHVAKLAKLAFADEELASFTDQLGKIIDMVEMLEEV
                     DTEGVPFTSNVVETVNVMRQDRAQAGWNRDELLKNVPETEDGFIKVPAIIDNGEAGA"
     gene            complement(6049..8085)
                     /gene="ligA"
                     /locus_tag="SCJ86_001208"
     CDS             complement(6049..8085)
                     /gene="ligA"
                     /locus_tag="SCJ86_001208"
                     /EC_number="6.5.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303894.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent DNA ligase LigA"
                     /protein_id="HEG1168782.1"
                     /translation="MEHEMTIDEAAKRAEELRTRLNQWSREYYVEDKPTVEDYVYDKE
                     YAELVAIEEQYPDLITSDSPTQRVGGKVLEGFEKVTHDIPLYSLNDVFSKEELIAFDQ
                     RVQKAVGRVVDYCCELKIDGLSVSLRYEDGNFVRGATRGDGTVGENITENLKTVRSVP
                     IKLKEPMNIEVRGECFMPKRSFVQLNQDREAEGKDIFANPRNAAAGSLRQLDSKITAK
                     RNLDTFLYTVADFGPMQAKTQYDALEELEKIGFHTNREKRLCHSIDEVWSYIEEYHDK
                     RVDLPYEIDGIVIKVNEFSLQDQLGFTIKAPRWAAAYKFPPEEVETLIENIEWTVGRT
                     GVVTPTAIMTPVRVAGTTVSRASLHNGDYIKLKDIRLKDTVLIYKAGDIIPEVSQVVL
                     DKRPKDSEEYQLPTHCPVCGSELVHLDEEVALRCINPKCPAQMKEGLNHFVSRNAMNI
                     DGLGPRVLEQMYDKKLVADVADLYKLTEEELLTLDKIKEKSANNILTAIDNSKDNSVE
                     RLIFGLGIRHVGAKAAKILAEHFGDLETLSKSDYESIIALDTIGDIIADSVVTYFSNE
                     EVHELMNELKQAGVNFEYKGLRNAQLQEVESPFKEKTVVLTGKLTRFTREEAKETIEN
                     LGGKVTGSVSKKTDIVVAGEDAGSKLTKAQELGIEVWTEDQMADALAKSRSVEE"
     gene            complement(8102..10336)
                     /gene="pcrA"
                     /locus_tag="SCJ86_001209"
     CDS             complement(8102..10336)
                     /gene="pcrA"
                     /locus_tag="SCJ86_001209"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016176505.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA helicase PcrA"
                     /protein_id="HEG1168783.1"
                     /translation="MSSKAELLNGMNPRQKEAVLHTDGPLLLMAGAGSGKTRVLTHRI
                     AYLIEEKEVNPWNILAITFTNKAAKEMKERVNAILASGGEDVWVSTFHSMCVRILRRD
                     VDFIGYNRNFTIIDSSEQLTLMKRILKELNIDPKKYDPRSILGTISQAKNSLQTPQDF
                     AKMQGSYYEEIAAKCYAAYQKELQYNQCMDFDDLIMNTIRLFEEHPDSLTYYQNKFHY
                     IHVDEYQDTNHAQYTLVNLLAGRFRNLCVVGDADQSIYGWRGADMQNILDFEKDYPDA
                     AVILLEQNYRSTKNILSAANQVIENNSNRKPKNLWTENKEGNKITYYRADNERDETRF
                     IVDRMQEEIRSNHRNYGDFAILYRTNAQSRVMEETLLKANIPYKMVGGHKFYDRKEIK
                     DILAYLNVLVNPQDSISFERIVNSPKRGIGPGSIEKLRSFASLHEWPLLEAAQNVDLA
                     NIGGKAGQQLGAFGEMIQEVTQMIPYLTVTELTKEVLDRSGYLEDLKIQNTLEAQARI
                     ENLEEFLTVTQEFDKQFEQQNEEDADAPEEKLTVFLNDLALVSDIDNLEEDASQVTLM
                     TLHAAKGLEFPVVFLIGLEEGVFPLSRALMEESELEEERRLAYVGITRAEEALYLTNA
                     FSRTLYGRTQYNRPSRFVEEIDQELLEIEGMRPTPKKTPVFAKKTAYSYKQPETAVVP
                     SKSATGGENNSWKPGDKVKHKKWGLGTVVRVSGTSKDLELDVAFPSQGVKRLLAAFAP
                     IEKA"
     gene            10491..12050
                     /locus_tag="SCJ86_001210"
     CDS             10491..12050
                     /locus_tag="SCJ86_001210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400269.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ClC family H(+)/Cl(-) exchange transporter"
                     /protein_id="HEG1168784.1"
                     /translation="MEKKGKIKKLDKTQIYFILKGIFVGLLTGIVVSLFRLSIEKLSD
                     LVRSIYEMSREQPIYLIGLAICCILAAFFVGYLVKNEPDIKGSGIPQVEGQLRGELSM
                     NWFSVLWKKFIGGVLSVGAGLFLGREGPSIQLGASIGQGAGQLFRSPSSEEKILISSG
                     ASSGLAAAFNAPIAGLLFVLEEIHHSFSPLVWLTSFASAITANFVSLYFFGLRPVLYL
                     GDLPSLPLKYYGSLVILGVLLGILGFIYQNVLLALPKFYNRLPLPAYFHGFVPFLLIL
                     PIGYLWPSTLGGGNQIVLAFGHASLPIIMIIGLFVLRFVFSMISYGGGLPGGIFLPIL
                     TLGALIGSFYGNIMVDFGMDPIYIKDFVVIAMAGYFTAIGKAPLTAIILVTEMVGSLN
                     HLMPLGLAALVAYIVNDLLGGNPIYESLLERLLGNTQTASLKGARTTFDFPVTAESSL
                     DGLMVRDFNWPKNMLLISIRRGNQEILTHGDTIMNVGDILVILTDEANLPRIKQEIEA
                     KSSITNLKQSF"
     gene            complement(12161..14092)
                     /locus_tag="SCJ86_001211"
     CDS             complement(12161..14092)
                     /locus_tag="SCJ86_001211"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400270.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HEG1168785.1"
                     /translation="MNIKDLMVKEAMIMDLQATDKKGAIDEMVQKMFDAGRISDIETY
                     KEGILAREAQTSTGLGDGIAMPHAKNSAVKEATVLFAKSQKGVDYEALDGQPTYLFFM
                     IAAPEGANDTHLQALAALSRLLIDADFVGKLKEANTPEEVQDLFQTAEEQKEAEEKAE
                     QETQAAEASAHPERKFVVAVTACPTGIAHTYMAEDALKKKAKEMGVEIKVETNGSEGI
                     KNRLTAEDIARAEGVIVAADKKVEMNRFDGKELINRPVSDGIRKPEELINLALSGTAP
                     VFHGDGKESSSEEGNADGTIGQRIYKDLMNGVSHMLPFVIGGGIAIALSFMIDQFIGV
                     PHDQLSNLGNYNQAASWFNQIGQAAFGFMLPVLAGFIASSIGDRPGLIVGFAAGALAN
                     TGGAGFLGALIGGFLAGYVIVFLRKLFKNLPKSLDGIKTILFYPVFGLLITGFLMLLV
                     NVPMKAINDALNGFLTSMSGSNAALLGALLAGMMAADLGGPINKAAYVFGTATLATTV
                     ATGGSVVMASVMAGGMVPPLAIFVATRLFKNKFSKTDQDAGLTNIVMGLSFVTEGSIP
                     FAAADPIRAIPSFIIGSALTGGLVGAFGIKLLAPHGGIFVVFLLSHPVMYLVFIAIGA
                     VVSGVIYGLLRKAPAVAEA"
     gene            complement(14110..15024)
                     /gene="pfkB"
                     /locus_tag="SCJ86_001212"
     CDS             complement(14110..15024)
                     /gene="pfkB"
                     /locus_tag="SCJ86_001212"
                     /EC_number="2.7.1.56"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288484.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1-phosphofructokinase"
                     /protein_id="HEG1168786.1"
                     /translation="MIYTVTLNPSIDYIVRVDGLKLGDLNRMKEDFKLPGGKGINVSR
                     ILKRIDSQSTALGFLGGFTGEFISKWLEKEQIQSLFTTVKEDTRINIKLKSDTETEIN
                     GLGPSISDEEIDELKQALGTVGQGDIVVLSGSAPASLRKGFYQELIDIIREKGADFVI
                     DTTGDDLKNALVKKPLLIKPNNHELAELYNTTFQSVEEIIPYGKKLLAEGAQNVLISM
                     AGDGALLFTEQGTYKTNVLVRPLKNSVGAGDSMIAGFIGAYSRTKDPIEAFKWGVACG
                     SATAFSDDLAARELIDELLPEVEITKID"
     gene            complement(15021..15773)
                     /locus_tag="SCJ86_001213"
     CDS             complement(15021..15773)
                     /locus_tag="SCJ86_001213"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288485.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR/GlpR transcriptional regulator"
                     /protein_id="HEG1168787.1"
                     /translation="MLTEERRQKILDLLEQQKIVKSQDLVNLLNASESTIRRDLQELE
                     NSGFLERVHGGAKKEQLLNFEQNMSEKSLKNVHEKQQIAKSAAETINDEDVIYLDAGS
                     TTLEMIPFLQGKQITVVTNSVQHAANLVDLELATIILGGTIKLATNAVLGSNAIEQLK
                     NFHFNKAFMGMNGAHLEQGFTTPDPEEAAVKRLAMKNSEETFVLIDHTKFNQLSFTSV
                     APLEAATIITERCPLEFVNEFREKTTIKEAIQ"
     gene            complement(15932..16141)
                     /locus_tag="SCJ86_001214"
     CDS             complement(15932..16141)
                     /locus_tag="SCJ86_001214"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294422.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEG1168788.1"
                     /translation="MDKQLYYRIMVPASLLSVCLFFVPNFPIRSVIGFLVMFGGMGLY
                     YYLLHKERKPASVYMKKTRSENKKR"
     gene            complement(16152..17480)
                     /locus_tag="SCJ86_001215"
     CDS             complement(16152..17480)
                     /locus_tag="SCJ86_001215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306500.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEG1168789.1"
                     /translation="MIGEDKLLMTIQLTDLNEEEKKQEISDWAILTRLLIQPTFIRSF
                     TQQADPYDLRKLQEKIMLASVRDESWLVVGNDENECSFRLEDNQLLIKNVLSISVFKE
                     KQFLIRDFIQKKMIAHGVFAYMRAYSEFIYHNTKQISQRLLFEKKDEIEHLPKMKQQN
                     GEVVVDCNQFPGYDLFYQGLCFTSCWEMYYSRYYHQIIPKPIFLDIQQVEKVKELENE
                     VICIQLYRDPFNWQKPNNQMFQSYFRDQLGFDHLAWDNGVGLLKPPFVEYIYTDHTIQ
                     SVQYQNAQMQPVPKKNASFFVTKSYDIQQGDYKERRVRGTLNAQAYFPWVDENRSRMM
                     CYKVIDPTVALDNGIEAYCYYIREYLEVEVTDEKYQEYLLSLRIYVPSESLSELPLKE
                     IKHRLSDVTFKRIKKKRRSIQFDVKKGEQHLRVQFLDYRELEQLITLQKI"
     gene            17616..17771
                     /locus_tag="SCJ86_001216"
     CDS             17616..17771
                     /locus_tag="SCJ86_001216"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288488.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEG1168790.1"
                     /translation="MGLKFERTDNLDKLFESFAVDPEKTVAPKEEIDRFLKPQPQNEK
                     KKDKKQK"
     gene            complement(17845..18432)
                     /locus_tag="SCJ86_001217"
     CDS             complement(17845..18432)
                     /locus_tag="SCJ86_001217"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306496.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YihA family ribosome biogenesis GTP-binding
                     protein"
                     /protein_id="HEG1168791.1"
                     /translation="MKVHQAEIIISAVAPKQYPDTQLPEIALAGRSNVGKSSFINTLI
                     DRKNLARTSGKPGKTQTLNFYLIENALHFVDVPGYGYAKVSKTERAKWGKMIETYLTT
                     RDQLRAVVSLVDLRHKPSQEDIQMYQFLKYYDIPVIVVATKADKIPRGKWNKHESEIK
                     KALDFDRNDTFILFSSVTKQGKEEAWAAIEQAIES"
     gene            complement(18515..19765)
                     /gene="clpX"
                     /locus_tag="SCJ86_001218"
     CDS             complement(18515..19765)
                     /gene="clpX"
                     /locus_tag="SCJ86_001218"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400278.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp protease ATP-binding subunit
                     ClpX"
                     /protein_id="HEG1168792.1"
                     /translation="MYDNPSSNETVRCSFCGKTQEEVKKIVAGPGVYICNECIDLCKE
                     IIDEEFYEEAVREFTEVPKPLEILEVFNNYVIGQDRAKRSLAVAVYNHYKRVNQQAQE
                     NTDDVELQKSNICLIGPTGSGKTFLAQTLAKTLNVPFAIADATSLTEAGYVGEDVENI
                     LLKLLQSADYNVELAEKGIIYIDEIDKIARKSENVSITRDVSGEGVQQALLKILEGTV
                     ASVPPQGGRKHPHQEFIQIDTTNILFIVGGAFDGIETIVKNRMGEKTIGFGTQNQKLS
                     EDESVMQHIIPEDLLKFGLIPEFIGRLPVMAALEKLTTDDLVRILTEPKNALVKQYQK
                     LLSLDNTELEFEPEALRAIAKKAIERNTGARGLRSIIEEIMMDVMFDIPSNENIEKVI
                     ITKEAAELSGEPTVIYKDSEKKAG"
     gene            complement(20012..21301)
                     /locus_tag="SCJ86_001219"
     CDS             complement(20012..21301)
                     /locus_tag="SCJ86_001219"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323981.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trigger factor"
                     /protein_id="HEG1168793.1"
                     /translation="MTATFEKKGTNDGVLKFTIAQDEIQKGLTTAFNKVKKSLNVPGF
                     RKGKVSRQVFNRMYGEEALYEDALNAVLPEAYEAAVKEAGIDPVSQPKIDVESMEKGQ
                     DWVITAEVTVKPEVKLGQYKDLVVEKQDREVTDADVDARIKREQEAQAELVIKEDAAA
                     ENGDTVVIDFEGFVDGEAFDGGKGENYSLELGSGSFIPGFEDQLVGHKSGESLDVNVT
                     FPEDYQAEDLAGKEAVFKVTIHEVKAKEVPELDDEFAKDVDDEVSTLDELKEKYRKEL
                     VETKEKAADDAKDEAAIRMAVENAEIVELPHVMVHDEVHRSMDEFLNNMQRQGISPEM
                     YYQLTGSTEEDLHKQFEGEAEMRTRTNLVIEAIAAAENLQATEEEINAEIQELSETYN
                     MPIEQIKRVLTEDMLEHDVTMKKAIELITGTATEAKE"
     gene            complement(21455..22387)
                     /locus_tag="SCJ86_001220"
     CDS             complement(21455..22387)
                     /locus_tag="SCJ86_001220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292071.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEG1168794.1"
                     /translation="MKPIDFPEKYENLMRVAQQALANQQYQQAKELFQRAYELKESFE
                     ANSLLVFCLYELDEKKEALKQALLHEKQYLQNEEFAEFYFDLLISTQDFLYARKLIAS
                     TDFYEAFEQRVIEKIQFAEELSGQMERQKIKALHNKSEELPSLEPARQLASIDEIEQL
                     PYHEFIQTASKLIVSPEVHILARAKLLETLRQLNECHPVFYLTIEEKLVKVIPKDLPK
                     PQQQASYQQLCVFSDRYGNEDALLSSVLKEEFTLQSAIVYPVYDTYIEDPKRWFQLTV
                     EACTGKTMYEGTEDEKQDFLKKREKILQQMIFFH"
     gene            complement(22544..23203)
                     /locus_tag="SCJ86_001221"
     CDS             complement(22544..23203)
                     /locus_tag="SCJ86_001221"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RDD family protein"
                     /protein_id="HEG1168795.1"
                     /translation="MSELSNENKEQEKDKQQQPAVGPDSNFSQKVLSSFEREPLTPEE
                     IKKKQRKWKKYTDTRNEPPKANDFPKYFYAGFFIRMFAFLVDLLCIGAITRLTLGIAK
                     NLGWLNFSDSYLSLYGFTALLIYLAYFILLTKFNHGQTIGKMIFGIRVISFDEEELSW
                     STVLVREGACRFILKFPLLMLGYLPAVFNQKKQHLGDFFTNTSVVTINLIKAFNQEVN
                     S"
     gene            complement(23206..24231)
                     /gene="sppA"
                     /locus_tag="SCJ86_001222"
     CDS             complement(23206..24231)
                     /gene="sppA"
                     /locus_tag="SCJ86_001222"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337680.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptide peptidase SppA"
                     /protein_id="HEG1168796.1"
                     /translation="MNKRRWAAVLIAAGLFVVSLVSASLTKPQENDQIEGLNSWLYGD
                     EELAPNVLEEGDSTQKIVKLSVDGTIAAGGSSGLFASEGYNHKNFMEQLKAIEEDPTV
                     KGIFLEVNSPGGGVYESAEIAKKLDTIRKEHDIPMYVSMKNMAASGGYYISAQADKIF
                     ATEETVTGSIGVIMSGLNYSGLLEKLGVEDTTVKSGALKDMGSGTRPETKEEEAVLQA
                     YIDSAYSRFVAIVSEGRNKSEEEVKKIADGRIYDGVQAKEVGLVDELGFPEDALKAIR
                     KEQQLEDAELVEYSSTPSGFASTWLGSKLAEFQGLKPSETSQILSVLENLGTAESPKA
                     MYYYGGE"
     gene            complement(24244..24534)
                     /locus_tag="SCJ86_001223"
     CDS             complement(24244..24534)
                     /locus_tag="SCJ86_001223"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEG1168797.1"
                     /translation="MDSKIEIMTLGMLKKQLSKFEASAGVSDDTKIFLDTGWDSIQEI
                     APDALEVAQAREFTVEDELTKESFSGYAREEKAERFDTSEQSETVIVIKNLY"
     gene            24943..26748
                     /gene="glmS"
                     /locus_tag="SCJ86_001224"
     CDS             24943..26748
                     /gene="glmS"
                     /locus_tag="SCJ86_001224"
                     /EC_number="2.6.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008267074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine--fructose-6-phosphate transaminase
                     (isomerizing)"
                     /protein_id="HEG1168798.1"
                     /translation="MCGIVGMIGLKNVTPGLIDGLEKLEYRGYDSAGIFVSDGTTDYL
                     VKAQGRIQNLKNKINIDTTGNIGIGHTRWATHGQPSEENAHPHTSQSGRFVLVHNGVI
                     ENFEELKIAYLANDHFIGETDTEIIAHLIETFAKDTTTQEAFLKALRVIKGSYAFALI
                     DRTAPDVIYVAKNKSPLLIGLGDGFNVIASDAMAMLAHTKEFVEIEDEEMVTVTSEKV
                     IIQNFAGDIVERSSFEAQVDASDIEKGTYPFYMLKEIDEQPIIMRRIAQKYVTEDNRV
                     SLDQKLVDTLSDSDRIYIVACGTSYHAGLAGKQTLEKLTQIPVEVHLASEFGYNTPLL
                     SKKPFFIFLSQSGETADSRQVLVKINRLGYPSLTITNVAGSTLSREASFTLLLHAGPE
                     IAVASTKAYTAQIAVLTLLAKAIGDKKEFETSLVFDITHELSLVANAMESVIAQKDYL
                     EELAEVYLSDTRNAFYIGRGADYNVSLEAALKLKEISYIQAEGFAAGELKHGTIALIE
                     EGTPVIGIISEEVTGAHTRGNLKEVESRGAKTLVIVSEGLEKESDQLVLPTVHPYLTT
                     LTTVVPTQLLAYYATLLRGFDVDKPRNLAKSVTVE"
     gene            complement(26820..28175)
                     /locus_tag="SCJ86_001225"
     CDS             complement(26820..28175)
                     /locus_tag="SCJ86_001225"
                     /EC_number="5.4.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010739004.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglucosamine mutase"
                     /protein_id="HEG1168799.1"
                     /translation="MGKYFGTDGVRGEANKELTPELAFKLGRCGGYVLSQHEEEGKRP
                     RVLVGRDTRISGQMLEEALISGLLSVGIEVFQLGVISTPGVAYLTRLQKASAGVMISA
                     SHNPAQDNGIKFFGADGFKLVDDQELEIEALLDAEVDELPRPSAEGLGTVEEFPEGLL
                     KYSQFLVQTINGDLSGLTICVDAANGATSTSVNRLFADLETDFYTMGTNPDGLNINDG
                     VGSTHPERLAEMVVEKGADAGLAFDGDGDRIIAVDELGNIVDGDKIMFICAKYLAEKN
                     RLKKDTIVTTVMSNLGFHKAVEGIGLKDVVTQVGDRYVVEEMRKNDYNFGGEQSGHMI
                     FLDFNTTGDGMLSGIQLLNIMKQTGKKLSELAAEVTIYPQKLVNIRVSDKNGAMDVPA
                     IKKVIEEAEAEMGSEGRILVRPSGTEPLLRVMAEAPTDEKVNYYVDKIAAVVKEEIGL
                     D"
     gene            complement(28199..29368)
                     /locus_tag="SCJ86_001226"
     CDS             complement(28199..29368)
                     /locus_tag="SCJ86_001226"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738803.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEG1168800.1"
                     /translation="MISKEKRTSILYSFIALLFSIALYFNANGQSVPNTLSGNEAYNQ
                     TVTGVPIKISYDSKRYYIHGYENAVTVKLSSANRVQLNAEANEDTRMFRVTADITKLG
                     EGTHEVPLKIQNLSSAVTAKIEPSTITVTVEKKVIKDFDVETLIPSTFTPSGYELDDT
                     SVSPKKVSITTGDKTLAEIKRVVADVDASMVTDDGINSEVPIQALNAAGEVLSIVSDP
                     VQVTVRADSIKPSKSVRLYGIQQGTPAAGVESYDFSFSKLEAEVSGSSDLLASIGDSI
                     AVPINVSGINHRTTRKIEIPVEEGLSIKPESVSVEITPVLQTSSSSSASTSSRTQENT
                     STSRSISSADSGTEPSTTTISSSSESGTSSSTTSTEETSETSESTQMSRTNSSEN"
     gene            complement(29365..30252)
                     /locus_tag="SCJ86_001227"
     CDS             complement(29365..30252)
                     /locus_tag="SCJ86_001227"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005880019.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR00159 family protein"
                     /protein_id="HEG1168801.1"
                     /translation="MSFQIGELFNLDYWQQMFSGDLFSLNFLVNILDILVVWYLVYKL
                     IQLLRGTKAIQLLKGVAIFIVIRIVAEIIGLHTLSWLMNQVITYGVIAAIVIFQPEIR
                     RGLEHLGRSSLFKQSKSEQQEDEKMILSFDKAIQYMSKRKIGALITIERHTGLDEYIE
                     TGIALDADITGELLINIFIPNTPLHDGAVIVKEGKIAVASAYLPLSESMLIPKEFGTR
                     HRAAVGISEVSDAITIVVSEETGDVSITLDNELMAGLSQQEYLAILRRELIAEEKTKN
                     KKNVIQSFLDGMTKGGDRK"
     gene            complement(30441..34133)
                     /gene="nifJ"
                     /locus_tag="SCJ86_001228"
     CDS             complement(30441..34133)
                     /gene="nifJ"
                     /locus_tag="SCJ86_001228"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327830.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate:ferredoxin (flavodoxin) oxidoreductase"
                     /protein_id="HEG1168802.1"
                     /translation="MRKMKTMDGNTAAAYISYAFTEIAAIYPITPSSTMAELVDQWSA
                     AGKENVFGQPVKVVEMQSEAGAAGVVHGSLKTGALTTTYTASQGLLLMIPNMYKIAGE
                     LLPSVFHVASRAVTTNALNIFGDHGDVMAARQTGFAMLAESSVQEVMDLAPVAHLASI
                     EGEVPFLNFFDGFRTSHEIQKIEVLEYEELAPLINQEKLASFRKRGMNPNHPTVSGTN
                     QNPDIHFQQRETINPYYDKLPEIVQKYMKEINRLRGTSYDLVTYYGADDAEEVIVSMG
                     SVAQTIEQTIDYLNANGRKTGFLNIHLYRPFPVETFVQKLPHTVKTIAVLDRSKEPGA
                     GGEPLLLDVQSALYDSELRPAVIGGRYGLGSKDVTPDQISAVFDELKKDPSIRKKRFT
                     IGIVDDVTYQSLEPKESLDLTEPQTFQAKFWGFGSDGTVGANKSAIKIIGDHTDKYAQ
                     GYFYYDSKKSGGLTVSHLRFGDTPIRSAYLVEHADLVACHTPAYLHSYDLVKGLKPGG
                     IFLLNTLWSDEQLETHLPLKLKRYLAENNIRFYTINAMRLAQEVGLGRRINTAMETAF
                     FKLADIIPFDEVLPLLKEEALKSYGHKSMAIVEKNIQAIDRTTELLHQVAIPDHWRTL
                     EMPEKINKRNTGYIQEILEPVNAQEGNQLSVGTLVANGMTTGEMPLGTASMEKRGVAL
                     EVPEWISDRCTMCNECAFVCPHAAIRPFLADEEEMEEAPEGFIVREMRGADGVKYRIQ
                     VSVEDCTGCGLCVEACPAKEKALVMKPYEEQKEQAVNWAFAMTLRQKANPVKGKNTVL
                     STQFNQPLLEFSGACSGCGETPYVKLLTQMFGDRMMIANATGCSSIWGGASPASPYTT
                     NECGQGPAWSNSLLEDNAEFGYGMLLAAQTRREALASSIKEVLPKVSEELRELMEDWL
                     VHMFESEGTQQRAAKLQAAIESERSIFPELEDIYKRKDLFVKNSQWMIGGDGWAYDIG
                     FGGIDHVLASGADVNILVLDNEVYSNTGGQTSKATPASAIAKFSASGKYASKKDLGMM
                     AMTYGNVYVAQIASGANQMQTIKAFEEAEKYPGPSLIIAYTPCITHGLLGGMKESLKE
                     AKEAVSSGYWSLYRYNPTMVEKNKNPMTLDYKKPDFSAMPDFMRKQVRFSSLDNANPN
                     LAKALFEKTVHDAKTRFYNYAALTGELEKIKKRLEPEESIDVPVKTRKEKTTDLEAEE
                     RRAARRAARAERRKKMEQATKEKKEK"
     gene            34332..34853
                     /locus_tag="SCJ86_001229"
     CDS             34332..34853
                     /locus_tag="SCJ86_001229"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323975.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DsbA family protein"
                     /protein_id="HEG1168803.1"
                     /translation="MDISVIDATKTNTQKGILYGSSNAPKKMVEFINLACPYCRQWFE
                     ESYELLEEAVQSGQLQRVIKLFDKEKESLQRGNVMHRYLTISDGQKAIKEIKQIFDTQ
                     DEWKHLSLQEVADFAVDKLKLTEQKDEQLSQAVINEAEQAHIRFVPTVILGKEIFDES
                     ISIEELKELIQTK"
BASE COUNT         9427 a         7587 c         6298 g        11632 t
ORIGIN      
        1 taaaaagact aagaaacaat aacaaaagtt aacacttctt agtctttctt tagcacgtct
       61 atttattaaa gattttaggg ccttttctca tgtttttacg atcattctta ggtgtttcca
      121 tttgtttgga tcggcctcca ccttgtttct ttttgatcaa ttctttcatt ttttcttttt
      181 ggtccatttt tattctcctt taacgaatga agcgactaat tcgatatgat gggtttgcgg
      241 gaaaagatct acaggctgaa ctttcttcac ggcataacct gcttctgcaa ataacttagc
      301 atctcttgca aaagttgccg gattacatga gatatagacg attcgttctg gtgacatttc
      361 gattgctgat tcaataaaat gatgatctaa tccttttcgt ggagggtcaa ctacgatcac
      421 agatggtttg atcccttttt cttgccattt tttcataact ttttctgctt ttccgacttc
      481 ataatgaaca ttttcgattt tattatttaa agcattgaac tgtgcatctt caattgcttc
      541 tggaataact tccatcccat acaccttatc cactctgtct gctaaagaca agccaatcgt
      601 acctattcct gaataagcgt cgatcacaat gtctttttta tctaactcgg caaactcgat
      661 ggctgtttgg tacagatact ctgcttgttg agtatttact tgataaaatg actttgctga
      721 gattcgatag gtttttccta atagctgatc ttcgatatag tctctgccat ataaaacttt
      781 ttctttgtct cctaaaatca catttgtttt ttcttcattg atattttgga tgaccgaaac
      841 cacttctggc aattcttcat gaatagcttc agcaatttcc ttggatttaa agaatttttc
      901 tttacgagta actaaaacga ccatcatttc atgggaataa tgaccacgac ggataatgat
      961 gtgacgaagc tgtccttcat ttttttcttc attatatcct cgtacttgga accgttgcaa
     1021 gatatctctg actactttga ttgcttgatc gatgacagga tcttggatat aaaaatcttc
     1081 gatagggact agatcatggc tattcttttt gtaaaaaccg gtggctaact gtccatcgat
     1141 acggcgaaca gggatctgtg ctttattacg gtagccgaat ggttctgcca ttccgaccgt
     1201 tggaagtaca ggaacttctg gcatcttagc aatcttcgcc attacatgct ctacttgttt
     1261 ttgtttgaag acaagctgtt gttcatactt caaatgggct aaaggtgcga tacctatgcg
     1321 aagtaatgca ttgttttcta caggctgacg atcaggtgat tgtgtaataa atgtttcgac
     1381 ttttccatat ccaaacttat tgcctgcttt tactactagt atttctactt cttctccagg
     1441 gagtgcattt tcgataaata atggataacc atcgattttt gctaccccca ttccttcata
     1501 actcaagtcg acgatttcga ctgtatatcg ttcgtttttc tttactattt gctgttctgt
     1561 catattttgc tcctttaagc ctatttttca atcctactga actctactat aaatcagcta
     1621 attacacaac taaaaccggc actgacctta ctacgttcag caccggcttt tgattaattc
     1681 ttttgattag gtgtttcgtc tgctattttt ccgtcaccgt cgatatcttc atccgttaaa
     1741 cgttcgactt cttttacgaa ttctttgctg acaacctcta attcttcttc atctgtacct
     1801 gtgattgctt catcaggaat ctgatcggta ttggcaaaga actcaatgtg ttgatgaaga
     1861 ttttggaaat gcatcggtgc ttccccgccg tattcaccat ctaagttgat cattactggt
     1921 cgattgatat catcttctgt ttcaacgaac aaactgcttg ttttggtata gattattctt
     1981 ggatcatcga catgtcgacc gccattcagc atgagtgctg caatatgcaa gatctcaaag
     2041 atatttgctg tcttcacaat gattaaagaa aacttcccat catccaattt ggcatctggc
     2101 gcgatctgtt cgaatcctcc aacagagttc gtcagcccta ggaaaaacat cgaagcattt
     2161 ccgatatatt ccccttcatc atacttcaag cgcattttga ttggctttac tcgagggagc
     2221 atctctgctc ctttcgcaag ataagcaaga taaccaaaaa tactctttag ttctgatgga
     2281 acttcataag tcaattctgt caaatagcca cctgcagcga tattgatgaa gtaactgtta
     2341 tcagaaaggc cgatgtccat ttttacggtt tggtgttttt ttatcacttc tgctgctgct
     2401 ttaatattat ctctaggaat ttttaatgct cgagcatagt cgttcgttgt accagctggg
     2461 atgatggcca ttttaggacg gtgttcaagc ggagcgattc cattaaccac ttcgttgatt
     2521 gtgccatcgc caccagctgc cactactaga tcaaaaccta gttctgcgac tctacatgct
     2581 tcattcttgg ctgaatctgg ttctggtgta gttgcatagg cgctggcttc aaatcctgct
     2641 tcttctaatg cctgtaggat atccgccaaa ttttttttca ataattcttt tcccgaggtg
     2701 gggttataaa ttacgcgtgc ttttttcatt ttcagcctct atcgtttcgc taattcatct
     2761 ttaagtagct tattcacgac accaggattt gcttgtcctt tagttgcttt catgatttga
     2821 ccgactagga agccaaccgc acgatctttt ccatttttga aatcatcgac agattgttgg
     2881 ttgttatcca aaatttcatt aatgattggc agtagtttgt tcggatcaga taattgcacc
     2941 caaccatttt tttcaactac ttcttgggct tctccgccat ttgtgatcaa ttcacggaag
     3001 acttttttgg cgatttttga gctgatcgtt ccatcggcga tcaaacgaat cattcctgct
     3061 aagttctcag gtgtcaattt tgtctcagct aattccagtt tctcactgtt caagtaagca
     3121 gaaacttcac ccatcaacca gttagatgct tgcttggcat ctgcaccatt ttccaaaaca
     3181 gcttcaaaaa aatcagacat ttcttttgta agtgtcaata ccattgcgtc atactctgga
     3241 agtccaaact cacggatata gcgttcgcga cgagaagctg gcatctcagg caaactagct
     3301 tttacttttt gaatccattc gtcatcgatc acgaatttcg ggatatcagg ctctgggaag
     3361 taacgataat cactagaccc ttctttgaca cgcatcaaaa ctgttttatt cgttgtttcg
     3421 tcaaaacggc gtgtttcttg ttggatttct cctcctgaaa gaagaacttt tgcttgacgt
     3481 ttttcttcaa aagctaaacc ttttttcacg aaagataaag agttcaagtt tttcaattct
     3541 gctttcgtac cgaactcttc ttgtccataa gggcgaagag aaatattggc atcacagcgc
     3601 atagagcctt cttccatttt cacatcgctg acttctgtaa attggataat tgatcgcaat
     3661 gcttctagat aagcgtatgc ttcttctggt gaacgcatat cagcttcaga aacgatttcg
     3721 atcaaaggcg tgccttgtcg gttcaaatca acgtaagaat aaccgccaat accatgcatg
     3781 ttttttccag catcttcttc taaatggaca cgttcgatcc ggattttttt ctttttacct
     3841 tctacttcga tttcgatcca tccgtcatga ccgattggtt gatcaaattg ggaaatttga
     3901 tatgcttttg gattatctgg gtaaaagtaa tttttccggt caaaatgagt atctttagag
     3961 atttcacagt ttaatgccaa agctgctttc atcccgaatt ccagcgcagc tttattcatt
     4021 actggcagca cgcctggata accccagtcg atcacgtttg tattgctgtt tggttctgca
     4081 ccaaaatgag ctggcgctgg agagaaaatt tttgagttgg tttttaactc gacgtggact
     4141 tctagtccaa tgactgtttc aaaattcatt cgtctttccc tcctaaaata gctggttgtt
     4201 gtttatggaa ttctgttgct tgttcaaacg catatgccgc ttgatacatc gttttttcat
     4261 caaaataatt accgatgatc tgcaatccaa ctggtaatcc ttctgataaa cctgccggta
     4321 cagacatacc tggaagtcca gccaagttga cagggatcgt taagatgtca ctcatgtaca
     4381 ttgtgattgg atcgttaatg ttctcaccga ttccgaatgc aactgttggt gaagatggcc
     4441 cgatgatcaa gtcataatct gcaaagactt tttcaaaatc tcgtttgatc aatgtacgga
     4501 cttgtcctgc ttttttgaag taagcgtcat aataacccgc gcttaaggag aatgtaccta
     4561 acatgatacg gcgttttact tcttcaccga atccttctga acgtgagttg acatatacat
     4621 catctaatga tttcacattt tcagagcgat aaccgtagcg gataccatcg aatcgttgca
     4681 aattggaact tgcttctgat gaggcaatga tataataaac agccacaccg tattttgaat
     4741 gaggcaagct gacttcttcc acaacagcac ctaatgaacg gaatgtttct gctgctttca
     4801 atacagcttc ttttacgcca ggggccacac cttcgcctaa gtattcttta ggtaaagcaa
     4861 tcttcattcc tttgatgtcg cctgtcagtc cctctgcaaa atcaggaact gaaacgcctg
     4921 aagaagtacc gtctttttcg tcataaccgc tgatagctgt caatgctaat gcattatctt
     4981 tcaccgtacg ggtcattggt ccgatttggt caagagatga agcaaaggca atcaaaccaa
     5041 aacgtgagac acggccataa gtaggtttca tccctacgat gccgttaaaa gctgctggtt
     5101 ggcggatact tcccccagta tcactaccta aagaaactgg aatttgtcca gctgcaactg
     5161 ctgctgcaga accaccagaa gagccaccag gtacttttgt tgggtcccaa gcattttttg
     5221 ttttcttgaa ataagaagtt tctgtacttc cacccatagc aaattcatcc atgtttagct
     5281 taccaacagg gatcatatct gcttgataga ttttatccat gaccgttgca tcataaattg
     5341 gttcaaaatt gtaaaggatc ttagatgcag cggttgtcaa gatatctttt gttacgatat
     5401 tatccttgat accaattgga ataccagcaa ggacgttgct ttcatcaatc cctttttcat
     5461 ctaaagcttt tgcctgttcc aatgcttttt cttcgttcaa tgtaatgaat gcctcgacct
     5521 caggctctgt ttctttgatt cgttcaaatg ttgtttttgt caaatcttgt acagtgattt
     5581 ctttcgatac aagcatatga tgcagttctt ctagagaacg attgtgtaat tctgtcatta
     5641 tgcgccagcc tccccattat cgatgattgc gggtactttg ataaagccgt cttctgtttc
     5701 cggcacattt ttcaataatt catcgcggtt ccatccagct tgcgcccgat cttgtcgcat
     5761 cacgtttact gtttcaacca cattcgacgt aaatggaaca ccttccgtat ctacttcttc
     5821 cagcatttca accatatcga tgattttacc taattggtcg gtaaaacttg caagttcttc
     5881 atctgcgaac gcaagttttg ctaatttggc aacgtgtttc acttgttctt cactaatcgc
     5941 catactaccc cttctttcta aacgagttaa aaactttgct ctctatttta acacaagagt
     6001 gccgtgtctg aaattgttta ttgaaatttg tctgaaaaaa ctcatttttc attcttctac
     6061 tgagcgactt ttagccaatg catctgccat ttgatcttct gtccaaactt cgattcctaa
     6121 ttcttgtgct ttggtcagct tgctgccagc atcttctcct gctaccacga tatccgtttt
     6181 tttcgatacg ctaccagtca ctttcccgcc aagattttcg atcgtctctt ttgcttcttc
     6241 acgagtaaat cgtgttagtt taccagtaag gactactgtt ttttctttaa aaggagactc
     6301 gacttcttgc aattgggcat ttcgaagccc tttgtattcg aaattgacgc ctgcttgttt
     6361 taattcattc atcaattcat ggacttcttc gttactgaaa taagtgacta cactgtcggc
     6421 aatgatatca ccgatcgtat caagcgcaat aatcgactca tagtcacttt tactcaatgt
     6481 ttctagatca ccgaaatgtt ctgctaaaat ttttgctgct ttcgctccga catgtcggat
     6541 tcctaacccg aaaatcaacc gctcgaccga attatctttg ctgttgtcga ttgctgtcaa
     6601 aatattattt gctgattttt cttttatttt gtccaacgtc aataactctt cttctgtcaa
     6661 tttgtaaaga tcagcaacat ctgctactag ttttttatca tacatctgtt ctagtacgcg
     6721 cggccccaac ccatcgatat tcatcgcatt tcttgaaaca aagtggttca aaccttcttt
     6781 catttgagca gggcatttag gattgatgca ccgtaaggct acttcttcat ccaaatggac
     6841 aagttcactt ccacaaactg gacaatgtgt aggcaattgg tattcttcgc tgtctttagg
     6901 tcgtttatct aacacgactt gcgaaacttc tgggatgatg tctcccgctt tataaatcag
     6961 aactgtatct ttcaagcgga tatccttcaa cttaatataa tcaccgttat gcaggctggc
     7021 acggcttact gtcgttcctg ctactcggac tggtgtcatg atagctgttg gcgttactac
     7081 ccctgttcga ccgactgtcc attcgatgtt ttcgattagc gtttcaactt cttctggcgg
     7141 aaacttgtaa gctgctgccc atctcggtgc tttgatagta aaaccaagtt gatcttgtag
     7201 cgagaattca ttcactttga tgacgattcc atcgatttca tagggcaaat ccacacgttt
     7261 gtcatgatat tcttcaatgt atgaccacac ttcatcaatc gaatgacata gacgtttttc
     7321 ccgattcgtg tggaatccaa ttttttctag ctcttctagc gcatcatact gggtttttgc
     7381 ttgcatagga ccgaaatcag ctactgtgta taaaaaagtg tccagatttc ttttcgctgt
     7441 gattttagaa tctaactgac gcaaagaacc agctgcggcg ttccgaggat tcgcaaagat
     7501 atcttttccc tctgcttctc tgtcttggtt caattggaca aatgaccgtt ttggcatgaa
     7561 acattcgcct cttacttcga tgttcattgg ttcttttaat ttgattggga ccgaccggac
     7621 agtttttagg ttttcagtga tgttttctcc tacagttcca tccccgcgag tcgcacctct
     7681 cacgaaattt ccatcttcat aacgcaaaga aacggaaagt ccatctattt ttagttcaca
     7741 gcaatagtca actacacgtc ccactgcttt ttgtacacgt tgatcaaaag ctatcagctc
     7801 ttctttgctg aaaacgtcgt tcaaactgta aagcggaata tcatgagtga ctttttcaaa
     7861 accttctaat acttttccgc ctactcgttg agtgggagaa tcagacgtga tcaaatcagg
     7921 atattgttcc tcgattgcta ctaattctgc gtattcttta tcataaacat agtcttccac
     7981 tgttggttta tcttctacgt agtattcgcg agaccattgg ttcagtcttg tacgaagttc
     8041 ttctgctcgt ttagcagctt cgtcaatcgt catttcatgt tccatcattt accctccact
     8101 actaagcttt ttcaattggc gcaaaggcag ccaaaagacg ttttactcct tgacttggga
     8161 aagccacatc tagttctaga tccttcgacg tcccgcttac tcggacgact gttcctagac
     8221 cccatttttt gtgtttgact ttatctccag gtttccagct gttattttcc cctccagtag
     8281 cacttttaga gggaacaacc gctgtctctg gctgtttata gctatatgct gtttttttag
     8341 cgaatacagg cgttttttta ggcgtaggac gcatgccttc gatttccagt aactcctgat
     8401 cgatttcttc tacaaaacga gaaggtcggt tatattgcgt ccgtccatat aatgttctgg
     8461 agaaagcatt tgttaaatac aacgcttctt ctgctcgcgt gatccccaca tatgcgagac
     8521 ggcgttcttc ctccagttcg ctttcttcca tcaatgctcg agataaagga aaaaccccct
     8581 cttctagacc aatcaggaat acaactggaa attctaatcc tttggctgca tgcaaagtca
     8641 tcaatgtcac ttgggaagca tcttcttcta aattgtcaat gtcggaaaca agtgccagat
     8701 catttaagaa gaccgttagt ttttcttctg gtgcatcagc gtcttcttca ttttgctgtt
     8761 cgaattgttt gtcgaattct tgtgtgaccg ttaaaaattc ctccaagttt tcgatgcgtg
     8821 cttgtgcttc taatgtattt tggattttta agtcttctag atatccgcta cgatccaaaa
     8881 cctctttcgt caattctgtc actgtcaagt atgggatcat ctgagtgact tcttggatca
     8941 tctctccaaa tgcacctaat tgctgtcctg cttttccacc gatatttgcc agatccacat
     9001 tttgcgctgc ttcaagcagt ggccattcat gaagagaagc aaaactgcgt agtttttcga
     9061 tagatcctgg tccgatgcct cgtttgggcg aattaacgat acgctcaaaa ctgattgaat
     9121 cttgaggatt taccagcaca ttcaaatacg ctaaaatatc tttgatttct ttacgatcgt
     9181 agaacttgtg ccctccaacc attttataag gaatatttgc tttcaacaga gtttcttcca
     9241 ttacacgaga ttgtgcgttc gtccggtaca agatggcaaa gtcgccgtaa ttccgatgat
     9301 tgcttcgaat ctcttcttgc atccggtcta cgataaaacg ggtttcgtct cgttcattat
     9361 ctgcacggta gtaagtgatc ttgtttcctt ctttgttttc tgtccacaag ttttttggtt
     9421 ttcgattgct attgttttca atgacttggt ttgctgcgct aaggatgttt ttcgtggagc
     9481 gatagttttg ttctagcaaa attactgcag catctggata atctttttca aaatccaaga
     9541 tgttctgcat atctgcaccg cgccagccgt aaatactttg atctgcatct cccacgacgc
     9601 acaggttcct aaatctacct gccaataaat tgactagcgt atactgtgcg tgatttgtat
     9661 cttggtactc atctacatga atataatgga atttgttttg ataatacgtg agtgaatcag
     9721 gatgttcttc aaataatcga atcgtattca tgatcaggtc gtcaaagtcc atacattgat
     9781 tatattgaag ctctttttgg taagcagcat aacattttgc cgcaatctct tcgtaataac
     9841 tgccttgcat ttttgcaaaa tcctgagggg tctgcaggct gttttttgct tgagagattg
     9901 ttcctaatat acttcgagga tcatattttt tcggatcaat gttcagttct tttagtatcc
     9961 gtttcatcaa ggtcagctgt tcagaagaat cgatgattgt aaagttccgg ttatacccga
    10021 taaaatcaac atctcttctt aaaatacgca cacacatcga gtgaaaagtc gatacccaga
    10081 catcttcgcc tccagatgcg agaatcgcat tgacacgttc tttcatttct tttgcagcct
    10141 tattggtaaa agtaatcgct aggatgttcc aaggattgac ttctttttcc tcaatcaaat
    10201 aggcaatgcg gtgtgtcagc actctcgtct ttccacttcc tgcacctgcc attagcagta
    10261 atggtccgtc agtgtgaaga acagcttctt tctgacgggg gttcatcccg tttaataatt
    10321 cagctttgga tgacattaac aacatccttt cttttatcaa tcttgttaat tatagcattt
    10381 tcttggcttt cattaaacta gctagttcaa aatcatttgt ttctttttat ggatttttcc
    10441 ttgtcaattc gttgatttcg cgctaaatta aaagaaagcg aggcgtttga atggaaaaaa
    10501 aaggaaaaat caagaaatta gataagactc agatatattt tattctaaaa ggaatatttg
    10561 ttggactgct gacaggaatc gtcgttagcc tgtttcgatt atctatcgaa aagctaagcg
    10621 atttagttcg aagtatctat gaaatgagtc gggaacagcc gatttactta attggcctag
    10681 ctatttgctg catccttgca gcatttttcg tgggttatct ggtcaaaaat gaaccggata
    10741 tcaagggaag cgggattcca caagtagagg gacagcttcg cggggaatta tcaatgaact
    10801 ggttctctgt tttatggaaa aagttcattg gcggtgtact ttcagttggt gcgggccttt
    10861 ttctaggtag agaaggacct tctatacagc ttggcgcatc cattggacaa ggagctggac
    10921 aattgtttcg gagtccatct tcagaagaaa agattttaat ttctagcggt gcaagtagcg
    10981 gacttgcagc agcattcaat gctcctatcg caggattact ctttgtttta gaagaaatcc
    11041 atcactcctt ttctccactt gtttggctga catcatttgc ttcagcaatc actgctaact
    11101 tcgtctctct ttactttttc ggcttgagac ctgttttata tctaggtgac cttccttctt
    11161 tgccgttgaa atattatggt tccttagtga tcttaggagt acttctaggc atcttaggtt
    11221 ttatttatca gaatgtcttg ctggctttgc caaaatttta caaccgattg cctttaccag
    11281 cttattttca cgggttcgtt cctttcttgc tgattttgcc aattggttat ttatggccta
    11341 gtactctagg cggaggcaat caaatcgttt tagcttttgg acatgcttcc ttgccaatca
    11401 ttatgattat cggtttgttt gttcttcgat tcgttttttc catgatttct tacggtgggg
    11461 gtcttccagg ggggattttc cttccgatat tgacattggg ggctttaatc ggtagctttt
    11521 atggaaacat tatggttgat ttcgggatgg atccaatcta catcaaggat tttgtcgtta
    11581 ttgccatggc tggttatttc acagcgattg gaaaagcacc gctgacagca attattttag
    11641 tgacagaaat ggttggctct cttaatcatc tgatgccttt aggattggct gctcttgttg
    11701 cttatatcgt caatgacctt ctcggcggta atccaattta cgaatcatta ttggaacgtc
    11761 tacttggaaa tactcagacc gcctcactaa aaggtgcaag gacaactttt gattttccag
    11821 taacggcaga gagttcttta gacggattaa tggtacgaga cttcaattgg cctaaaaata
    11881 tgctgctaat ctctatacgc agaggaaatc aggaaatctt gactcacggt gatacgatca
    11941 tgaatgtagg agatattttg gttatcttga ctgatgaagc aaacttgcca agaatcaagc
    12001 aggaaatcga ggctaaatct tctatcacaa atttgaagca atcattttaa cgaacatgga
    12061 aaaactattt tacaacaact attcatctac actacttgat tctatatgat tctataaaaa
    12121 aagaagaggc tgggaaaacc cagcctcttc ttttttattt ctatgcttct gctactgcag
    12181 gagcttttct caataaacca taaatcacac cagatacgac tgctccgata gcaataaaca
    12241 ccaaatacat gaccgggtga ctcagtaaga atacaacgaa aatcccgccg tgaggagcca
    12301 gtaatttgat tccgaaagct ccgactaatc cgccagttaa agcagaacca ataataaaac
    12361 ttggaatggc acgaatcggg tcagctgctg caaatggaat cgatccttcc gtgacaaacg
    12421 atagtcccat cacgatgttc gtcaaaccag catcttgatc tgttttgctg aatttattct
    12481 taaacaaacg tgtagcaacg aaaattgcca atggaggtac cattcctcca gccataacag
    12541 aagccatcac gacacttccg ccagttgcaa cagtcgtagc taaagttgct gtaccaaaca
    12601 cataagcggc tttgttgatt ggaccgccta aatctgccgc catcattcca gctaataatg
    12661 caccaagtaa agcagcattc gaaccactca tacttgtcaa gaaaccgttc aatgcatcat
    12721 tgattgcttt catagggaca ttgaccagca acatcaagaa accagtaatc agtaagccaa
    12781 acactggata aaagaggatc gttttaattc catccaatga ttttggtaag ttcttgaata
    12841 atttacgtaa aaatacgatg acataaccag ctaagaatcc accaatcagt gcacctaaga
    12901 atcctgcacc gcctgtatta gctaatgcac cagcagcaaa tccaacgatc aaaccagggc
    12961 ggtcaccgat acttgaagca ataaatcctg ctaataccgg cagcatgaaa ccaaatgcag
    13021 cttgtccgat ttggttgaac cagctagcag cttgattgta attccctaga tttgataatt
    13081 gatcatgtgg cacgccaata aattggtcga tcatgaaaga aagcgcaata gcgattcctc
    13141 caccaataac gaaaggtaac atgtgtgata cgccgttcat caaatctttg taaattcttt
    13201 gtccgatagt accatccgca tttccttctt ctgaactgct ttcttttcca tcaccatgga
    13261 atactggtgc agtaccacta agggctagat tgatcaactc ttctggttta cggataccat
    13321 cactgacagg acgattgatc agttcttttc cgtcaaagcg attcatctct acttttttgt
    13381 ctgctgcaac gattacacct tctgcacggg cgatgtcttc agcagtcaat cggtttttga
    13441 ttccttctga accgttcgtt tcgactttga tctccacacc catttctttc gcttttttct
    13501 tcaaagcatc ttctgccatg taagtgtgag cgatccctgt tggacaagct gtaacggcaa
    13561 caacgaattt tctttctgga tgagcagaag cttctgcagc ttgtgtttct tgttctgctt
    13621 tttcttctgc ttctttttgc tcttcagctg tttggaacaa gtcttggact tcttctggtg
    13681 tatttgcttc ttttaatttt cctacgaaat ctgcatcaat cagcaatcga gatagtgctg
    13741 ctaatgcttg caaatgtgtg tcgtttgcgc cttctggagc tgcgatcatg aaaaacagat
    13801 aagttggctg tccgtctaag gcttcataat ctactccttt ttggcttttt gcgaataaaa
    13861 ctgttgcttc tttaactgca ctgtttttcg cgtgaggcat ggcgattcca tcaccaagac
    13921 ctgtagaagt ttgtgcttcg cgggctagaa ttccttcttt atatgtttcg atatcggaaa
    13981 tccggccggc atcaaacatt ttttggacca tttcatcgat tgcgcctttt ttatcagtcg
    14041 cttgcaaatc catgatcatc gcttctttga ccattaaatc cttgatattc atgattatcc
    14101 tccttgaaac taatcaattt ttgtgatttc aacttctggc agcaactcat caatcaattc
    14161 gcgagctgcc aagtcatcag aaaaagctgt tgcgcttccg caagcaactc cccatttaaa
    14221 ggcttcgatt ggatctttcg tacggctgta agccccaata aagccagcaa tcattgaatc
    14281 ccctgcacct actgaatttt tcaatgggcg aactaataca ttcgttttat aagttccttg
    14341 ttccgtgaag agcaaagcac cgtctccagc cattgagatc agcacattct gtgccccttc
    14401 agctagtaat tttttcccat acggaatgat ttcttctaca gattggaagg ttgtgttgta
    14461 aagctcagct aattcgtgat tgtttggttt gatcaacaac ggtttcttaa caagtgcatt
    14521 ttttaggtca tcacctgttg tatcaatcac gaaatctgca cctttttctc gaatgatatc
    14581 aatcagttct tgataaaatc cttttcgcag gctagctggc gcgcttcctg acaatacgac
    14641 gatatctcct tgtcctactg ttcctaatgc ttgcttcaac tcatcgattt cttcatctga
    14701 aatagatggg cctaagccat tgatctcagt ttcagtgtct gattttagtt tgatattgat
    14761 tcttgtatct tcttttacgg ttgtgaacaa agactggatt tgttcttttt caagccactt
    14821 cgaaataaat tcgccagtga accctcctaa aaaccctagt gctgtagatt gagaatcgat
    14881 ccgtttcaaa atacgggaaa cattgatccc ttttcctcca ggtaacttga agtcttcctt
    14941 cattcggttc aaatcgccta gttttaaccc atctacgcgt acgatataat caattgatgg
    15001 attaagtgtt actgtgtaaa tcattgtatt gcctccttaa ttgttgtctt ttcacggaat
    15061 tcattaacga attctaatgg acaacgctcg gttatgatcg tggctgcttc caaaggtgca
    15121 acagaggtga aggacaactg gttgaatttg gtatgatcaa tcaatacaaa tgtctcttcg
    15181 gaatttttca tggctaaacg tttaacagct gcctcttctg gatccggagt cgtgaatcct
    15241 tgttcaagat gggcaccgtt cattcccata aatgctttat taaaatgaaa attttttagt
    15301 tgttctatcg cattagatcc taaaacagca ttagttgcca gctttatcgt tccacctaaa
    15361 ataatggtag caagctccaa atcaactaaa tttgccgcat gttgaacgga atttgtcact
    15421 actgtgatct gttttccttg cagaaaagga atcatttcta acgtagtaga gccagcgtct
    15481 aaataaatga catcttcgtc attaatggtt tcagctgccg atttggcgat ttgctgtttt
    15541 tcgtgaacgt ttttgagtga tttttcactc atattctgct caaaattcaa taattgctct
    15601 ttcttagctc cgccatgaac tctttctaga aacccactat tctccagctc ttgtaaatca
    15661 cgtcttatcg ttgattcaga agcattcaaa aggtttacaa gatcttgtga tttcacgatt
    15721 ttctgctgtt caagcaagtc taggattttt tgtctgcgtt cctctgtaag cattttcacg
    15781 atcacctcaa tacaaataat agcatatttt ttttctacta gcaatataaa tcagtcaaaa
    15841 acgttcataa aaattataaa tcagtcaaaa aaaaataaaa aaccagtcag aaagactggt
    15901 tttttatttt tttgtctatc atcaagccgt tttatcgttt tttattttcg cttcttgttt
    15961 ttttcatata aacagaagca ggttttcttt ccttatgaag aagataataa taaagtccca
    16021 tcccgccaaa catgaccaaa aaaccgatga ctgatcgaat aggaaaatta ggaacaaaaa
    16081 agagacagac ggataataat gatgccggaa ccattattcg ataatagagt tgtttatcca
    16141 ttttcttcac ctcatatttt ttgtaaagta atcaattgtt ctaattctcg gtaatctaaa
    16201 aattgcacac gcagatgctg ctcacctttc ttgacatcga attggatact tcgccgcttt
    16261 ttctttattc gtttgaaggt aacatcagag agtctatgtt tgatttcctt taacggtaat
    16321 tctgacaggg attctgaagg aacatagatt cttaaggata aaagatactc ttgatatttt
    16381 tcatctgtta cctctacttc tagatattcc ctaatataat aacaataagc ttcgattcca
    16441 ttatccaaag caacagtcgg atcgataact ttatagcaca tcatgcgtga tcgattctcg
    16501 tcaacccaag gaaaatacgc ctgagcattt agtgttccgc gaacacgcct ttctttataa
    16561 tctccttgtt ggatatcata actttttgtt acaaaaaatg aagcattctt tttaggaaca
    16621 ggttgcattt gtgcattctg atactggaca gattggatcg tatggtcagt gtaaatgtat
    16681 tcaacgaatg gcggtttcaa taaaccgaca ccattgtccc aagcaagatg atcaaaccca
    16741 agctggtccc taaaatagga ttgaaacatt tgattgtttg gcttttgcca gttaaagggg
    16801 tctcgataaa gctggataca tatgacttcg ttttctaatt ccttcacttt ttctacttgt
    16861 tggatgtcga gaaaaatcgg tttagggatg atttgatgat aatagcgact ataatacatt
    16921 tcccaacacg aagtgaaaca taaaccttgg taaaaaagat cgtatcctgg aaattggttg
    16981 caatcgacta ctacttcccc gttttgttgt ttcattttag gtaaatgttc gatttcatcc
    17041 tttttctcaa acaatagccg ttgagagatc tgttttgtat tgtgatagat aaactcgcta
    17101 taagctcgca tataggcgaa caccccatga gcaatcattt ttttctgaat gaagtctcga
    17161 atcaaaaatt gcttttcttt gaaaacagag atagacaata cattttttat caataattga
    17221 ttatcttcca agcgaaaact acattcgttc tcgtcatttc ctacaactaa ccaactttcg
    17281 tctcttaccg atgctagcat tattttttct tgaagctttc gaagatcata cggatccgct
    17341 tgttgtgtga aagagcggat aaatgttggc tggatcaaca gacgtgtcaa aatagcccaa
    17401 tcgcttattt cctgtttttt ttcttcttca tttaagtctg tgagctggat agtcatcaat
    17461 aatttatctt cgccaatcat tttctcacct ctttcaaatc tttttataat ttaattataa
    17521 ccaatgataa agaaaaacta aaactttatg tcttagaaac caatttggac gattacatga
    17581 tagactaagt aaaaagaaac aaagaggtgt ttacaatggg gttgaaattt gaacgtacag
    17641 ataatttaga taaacttttc gaatcatttg ctgtcgatcc ggaaaaaaca gttgcaccaa
    17701 aagaagagat cgatcgattt ctaaaaccac agccacaaaa tgaaaagaaa aaagacaaga
    17761 aacaaaagta agttaagctc aaaaaaagac ttaccagcca aacttttatg aaagagtttg
    17821 actggtaagt cttttttatt ctttttatga ttcaattgct tgttcgattg ctgcccacgc
    17881 ttcttctttt ccctgttttg taacggaaga aaagagaata aacgtgtcat tgcgatcaaa
    17941 atccaacgct tttttgattt cgctctcatg cttgttccat ttaccacgag ggatcttatc
    18001 tgccttagtg gcaacaacaa ttacagggat atcataatac ttcaaaaatt gatacatctg
    18061 gatatcttct tgagaaggct tatgacgcaa atctactaaa gaaacgaccg cacgtagctg
    18121 gtctctagtc gttaaatatg tctcgatcat ctttccccat tttgctcgct cagttttgga
    18181 tacttttgca tacccatatc ccggaacatc gacaaaatgt aatgcgttct cgatcaaata
    18241 aaaattcaat gtctgggtct ttcctggctt accggaagta cgggctaaat tctttcgatc
    18301 aatcaatgtg ttgataaaag aagatttccc tacatttgat cgtcctgcca gagctatttc
    18361 tggcaattgt gtatctggat attgttttgg tgccactgca cttatgataa tttcagcttg
    18421 atggactttc atggtctctc tccttaacaa ggtgatgaat aaagcaagcg gctgtcgcaa
    18481 aagccaaaat gacttttacg gcagcctctt ttcatcaacc cgcttttttc tctgaatctt
    18541 tatagattac tgttggctca ccagacaatt cagctgcttc ttttgtgata atcacttttt
    18601 cgatgttttc atttgaaggg atatcaaaca tcacatccat catgatttct tcaatgatgg
    18661 aacgaagtcc tcgagcaccg gtattgcgtt cgattgcttt tttcgcaatg gctctcaacg
    18721 cttcaggttc gaattcaagt tcggtattat ctagagaaag aagtttttga tattgtttga
    18781 ctagcgcatt tttcggctca gtcaagatcc gtaccagatc atctgttgtc aatttctcta
    18841 aagctgccat aacaggtaat cgtccgataa actcagggat caatccgaat tttagtaaat
    18901 cttcaggaat gatatgctgc atgacacttt cgtcttcaga aagtttttgg ttttgtgtac
    18961 caaatccaat cgtcttttcg cccatacgat tcttcacaat cgtttcgatg ccatcaaatg
    19021 caccacctac gatgaacaat atgttcgtcg tatcaatctg aataaattct tgatgtggat
    19081 gtttacgtcc gccctgaggt ggcacacttg caacagttcc ttctagaatt ttcagcaagg
    19141 cttgttggac accttcgccg gaaacgtcac gagtaataga tacattttcg cttttacgcg
    19201 caattttatc gatctcatca atatagatga tccctttttc tgcaagttcc acattgtaat
    19261 cagcagattg aagcaattta agcaaaatgt tttctacatc ttcccccaca taaccagctt
    19321 ctgtcaaact tgtcgcatct gcgattgcaa aaggtacatt caatgttttt gctaatgttt
    19381 gtgctaaaaa tgttttacca gaccctgtag gtccgatcaa gcagatattg cttttttgca
    19441 gttcaacatc atctgtgttt tcttgtgctt gctgattgac acgtttatag tgattgtata
    19501 cggcaacagc taacgagcgt tttgcgcgat cttgtccaat cacgtagtta ttaaatactt
    19561 caagaatttc tagtggttta gggacttcag tgaattcgcg tacagcttct tcataaaatt
    19621 cttcatcaat gatttcttta cataggtcga tacattcatt acagatgtag acccctggac
    19681 cagctacgat ctttttcact tcttcttggg ttttcccgca aaaagaacag cgtacggtct
    19741 cattgctact tggattgtca tacatggtct tcacccctta acttgcaaaa agtatgcaag
    19801 aaactatcaa agactttaca gtcttttact atcataacat agtttagtaa aaagaaaaag
    19861 tgtttaacaa aggatcaact gacctatacc tttgcatttt ttactcttaa caaaaaataa
    19921 ggaactgtga caaaatcgtg tcacagtccc ttatttcgaa taaacaattt tctagaagta
    19981 gcttcttcag aaatgattta ttacttgatt tctattcttt cgcttctgtc gctgtacctg
    20041 tgatcaattc gattgctttt ttcattgtca catcatgttc cagcatatct tctgtcaaca
    20101 cgcgtttgat ttgttcgatt ggcatgttgt atgtttctga taattcttgg atttctgcat
    20161 tgatttcttc ttctgttgct tgaagatttt cagcagctgc aattgcttcg atcacaagat
    20221 ttgttcttgt gcgcatttct gcttcacctt cgaattgttt atgcaagtct tcttctgtag
    20281 aaccagttaa ttgatagtac atttcaggag agataccttg acgttgcatg ttgttcaaga
    20341 attcgtccat tgaacgatgt acttcatcat gaaccatcac gtgtggcaat tcaacgattt
    20401 ctgcgttttc aacagccata cggatcgctg cttcatcttt tgcatcgtcc gctgcttttt
    20461 cttttgtttc tactaattct ttgcgatatt tttctttcaa ttcgtctaat gttgatactt
    20521 cgtcatcaac atcttttgcg aattcgtcat ctaattcagg cacttctttt gcttttactt
    20581 catggattgt cactttgaag actgcttctt ttccagcaag atcttctgct tgataatctt
    20641 ctgggaaagt tacatttaca tctagacttt cacctgattt atgaccaact aattgatctt
    20701 caaaacctgg aatgaatgag cctgaaccta attcaagtga gtaattttcg ccttttccgc
    20761 catcaaacgc ttcgccatct acaaagcctt cgaagtcgat tactactgta tcgccatttt
    20821 cagctgcagc atcttctttg ataactaatt cagcttgtgc ttcttgttca cgtttgatac
    20881 gtgcatccac gtcagcatct gttacttcac ggtcttgttt ttcaacaact agatctttat
    20941 attgtcctaa ttttacttct ggtttcactg ttacttcagc agtgattacc cagtcttgac
    21001 ctttttccat gctttcaaca tcgatttttg gttgagaaac aggatcgatg ccagcttctt
    21061 tgacagcagc ttcataagct tctggtaata cagcattcaa tgcatcttca tataaagctt
    21121 cttcaccgta catacggttg aatacttgac gagaaacttt ccctttacgg aatccaggta
    21181 cgttcaagct ttttttcact ttattaaacg cagttgttaa tcctttttgg atttcgtctt
    21241 gcgcaatcgt aaattttaaa acaccatcat tcgtgccttt tttttcaaat gttgcagtca
    21301 tttaattccc tccgagttta gaaattcatt ttatacatca aaaacgtttc atgcatacct
    21361 ttaaatagta cactaaccct ttcaaattgt aaacatattg aatggataga atccttgttt
    21421 ttcataacat ttttatcttt ttcactgata aaatctaatg gaagaagatc atttgctgca
    21481 aaattttttc tctttttttt aaaaaatctt gtttttcatc ttctgtacct tcatacatcg
    21541 tttttcctgt acacgcctct acagtcagtt gaaaccagcg cttcgggtct tcgatgtatg
    21601 tatcatagac aggataaaca attgcagatt gcaacgtgaa ttcttctttt aacacggaac
    21661 taagcagcgc atcttcattc ccgtaacgat ctgaaaatac acataattgc tggtaacttg
    21721 cctgctgctg cggctttggc agatcttttg gtatgacttt gactaacttt tcttcaatcg
    21781 tcaaatagaa aacaggatgg cattcgttca attgcctaag tgtctccagt aattttgccc
    21841 ttgctaaaat atggacctct ggcgatacaa taagtttact cgctgtttgg ataaactcat
    21901 gataaggaag ctgttcgatc tcatcaatag acgcaagctg acgtgcaggt tccaacgaag
    21961 gaagttcctc cgatttattg tggagtgcct tgatcttctg cctctccatt tgtccggata
    22021 attcttctgc aaattggatt ttttcaatga cccgctgctc gaaagcttca taaaaatctg
    22081 tacttgctat caattttcta gcatataaaa aatcttgtgt actgattaac aagtcaaaat
    22141 aaaattcagc aaactcctca ttttgaagat actgtttttc atgcaagagt gcttgcttca
    22201 atgcttcttt tttctcgtcc aactcgtaaa gacaaaaaac aagcaggcta ttagcctcaa
    22261 agctttcttt caactcgtaa gctcgttgaa ataattcttt tgcctgctga tattgctggt
    22321 ttgctagtgc ttgttgtgcc actcgcatta aattctcata tttttctgga aaatcaatcg
    22381 gcttcacgca gtgtcctcct cttgctcata ttcagctcat gttcaatagt agcaaaacaa
    22441 aaaaagcatg acaattaaaa ataactgaag caccctctaa aagctagatt ttagatctga
    22501 cttttagaag gtgctttcaa ctgttactgc tttttattgc tcatcagctg ttgacttcct
    22561 ggttgaacgc tttgatcaga ttgatcgtaa caacacttgt attagtgaaa aaatcgccca
    22621 gatgttgctt tttctgatta aagactgctg gaagataccc taacatcagc aaaggaaatt
    22681 ttagaatgaa ccggcaagca ccttctctaa ctaaaacggt tgaccagcta agttcttctt
    22741 catcaaagct gatgactcga ataccaaaga tcattttccc aatcgtttgg ccatgattga
    22801 atttcgtcaa taaaataaaa tacgctagat aaataagtaa ggctgtgaat ccgtagagac
    22861 tcagataaga atctgaaaaa ttaagccaac ctagattttt ggcaatacca agtgttaaac
    22921 gcgtaatagc tccgatacac aaaagatcga ccaagaaggc aaacattcgg ataaaaaaac
    22981 ctgcatagaa atacttagga aaatcatttg cttttggcgg ttcatttctt gtgtccgtat
    23041 actttttcca tttacgttgt ttctttttga tttcttcggg cgtcaaaggc tctctttcaa
    23101 aagagctcag taccttttgg gaaaagttgg agtcaggtcc tacagcaggc tgctgttgct
    23161 tatctttttc ttgttcctta ttttcatttg aaagctctga catgcttact cacctccgta
    23221 ataatacatt gcttttggtg attccgctgt ccctagattc tcaagaacgg ataagatctg
    23281 actagtctca gaaggcttca atccctggaa ttctgctagt ttacttccta accatgtact
    23341 agcaaaccca gatggcgtgg aagaatattc tactagttcc gcatcttcaa gctgttgttc
    23401 ctttcgtata gcttttaaag catcttcagg aaatcccaat tcatcgacta aacctacttc
    23461 ttttgcttgg acaccatcat aaatccttcc atcagcaatt tttttgactt cttcttcaga
    23521 tttattccgt ccctcgctga cgattgctac aaaacgacta tatgcgctgt cgatatatgc
    23581 ttgaagcacc gcttcttctt cttttgtctc tggtcgcgtt cctgaaccca tatctttcaa
    23641 cgctccgctt ttaactgttg tatcttccac acccagtttt tctagaagtc ctgaataatt
    23701 caatcctgac atgatcacac caattgagcc ggtcactgtt tcttctgttg caaagatttt
    23761 atctgcttga gcagaaatat agtagccacc gctagctgcc atgtttttca tgctgacata
    23821 cattgggata tcatgttctt tacgaatcgt atccaatttc ttagcgattt ccgcactttc
    23881 gtagacaccg ccaccaggag aatttacttc taaaaagata cctttgactg ttggatcttc
    23941 ttcaatcgcc ttcagttgtt ccataaaatt tttatgattg taaccttcag aagcaaataa
    24001 acctgaagac cctcccgcag caatcgttcc atctactgat aattttacga ttttttgcgt
    24061 actgtctccc tcttccaaca catttggagc tagttcttca tccccatata accaactgtt
    24121 caatccctca atttgatcgt tttcctgagg cttagtcaaa ctagctgaaa ctaacgaaac
    24181 cacaaataat cctgcagcaa tcagaactgc tgcccaacgt cttttattca ttctttctcc
    24241 tttctaatac aagtttttta tgacgatcac agtttctgac tgctcagaag tgtcgaaccg
    24301 ctctgctttt tcttcccgcg catacccgga aaacgactct tttgttaatt catcttctac
    24361 cgtaaactca cgagcttgag caacttccaa ggcatcaggt gcgatttctt ggatgctatc
    24421 ccagccagta tctaaaaata ttttcgtatc atctgataca ccagctgatg cttcgaattt
    24481 ggacaattgt ttttttaaca ttcccagtgt catgatctca atttttgaat ccatttttac
    24541 tcctcctcta tccgattact ttctattgta ccgaactcct gaaaaagatg atagataaaa
    24601 acccattatt ttttgacata aaaaggacat ttgacaaaga atttgatata taccaattta
    24661 ttttttattc tttttttgtt gacaagaaca ttcgcaaaag gttatgattt taaatgtttc
    24721 cagataaaaa acaaaaagga ggatagttat accttttagg atagcgccag gtctgtgtaa
    24781 acagatgacg aggagagggt ttatcgaaag attcggcgga tggccctagg gactgcactc
    24841 tacagaaaat ccacaaaaaa tgtctgcaaa gataaaacaa aaggattttc ctgcagctgg
    24901 aagcattcat acgaattcat tatagaaagt aggatgtttt ttatgtgcgg aattgttgga
    24961 atgattggat taaaaaatgt aacaccagga ttgattgacg gtctagaaaa attagaatac
    25021 cgtggttatg attcagctgg tatctttgtc tcagatggca caactgatta tctggtcaaa
    25081 gcacaaggca gaattcaaaa tttgaaaaac aaaataaata ttgatactac aggaaatata
    25141 ggtattggtc atacacgatg ggcaactcac gggcaacctt ctgaagaaaa cgcccatcca
    25201 catacttccc aaagtggacg cttcgtgctc gttcacaacg gcgtaatcga aaactttgaa
    25261 gaacttaaaa ttgcttattt agcaaatgac cactttattg gtgagaccga tactgagatt
    25321 atcgctcatc taattgaaac tttcgcgaag gatactacaa cgcaagaggc gtttttaaaa
    25381 gctttgcggg tcatcaaagg ttcttacgct ttcgctttga ttgatcggac agcacctgat
    25441 gttatttacg tcgctaaaaa taaaagtcca ctattaatcg gactcggcga tggattcaat
    25501 gtgattgcca gcgatgcaat ggctatgctt gctcatacga aggaattcgt tgagattgaa
    25561 gatgaagaga tggttaccgt cacatcagaa aaagttatta ttcaaaattt tgcaggtgac
    25621 atcgtagaac gttcttcatt cgaagcacaa gtagatgctt cagatatcga gaaaggtacc
    25681 tatccttttt atatgctgaa agaaatcgac gaacagccta ttatcatgcg tcgtatcgct
    25741 caaaaatacg taacagaaga caatcgtgtc tctcttgatc aaaagctggt cgatacattg
    25801 tctgatagcg atcgaatcta tatcgttgct tgcggaacta gttaccatgc ggggttagca
    25861 ggtaaacaga ctttggaaaa attgacacag attcctgttg aggttcattt agccagtgaa
    25921 tttggctata acacacctct tctttcaaaa aaaccattct ttattttctt gagtcaaagt
    25981 ggcgaaacag cagacagccg ccaagtgctg gtaaaaatca accgtctcgg ctacccttca
    26041 cttacgatta caaatgttgc tggttctact ctttcgcggg aagcatcctt tacactgcta
    26101 ttgcatgctg gacccgaaat cgcagtagca tcaacgaaag cttatactgc acaaatagct
    26161 gtactaactt tattagccaa agctatcggt gataaaaaag aatttgaaac ttctcttgta
    26221 ttcgatatca ctcatgaatt gagtttagtt gctaatgcga tggaatcggt catcgcccaa
    26281 aaagattatt tagaagagtt ggcagaagta tatctcagcg atacaagaaa tgccttttac
    26341 attggacgtg gtgctgacta caacgtttct cttgaagctg ctttgaaatt gaaagaaatc
    26401 agttatatcc aggcagaagg ttttgccgct ggcgaattga aacatggaac gattgctttg
    26461 atcgaagaag gaacaccagt gatcggaatc atcagtgaag aagtgactgg tgcgcatact
    26521 cgaggaaacc tgaaagaagt cgaaagccgt ggcgctaaaa cactagttat tgtctcagag
    26581 ggattagaaa aggaaagtga ccagctggtg ctaccaactg tccaccctta tctaactact
    26641 ctaaccacag tcgtaccgac tcaattactt gcatattacg caacgctatt acggggattc
    26701 gacgttgaca aaccacgtaa tttagcgaaa tcagtgacag tagaataata aaagaacaag
    26761 gaccagtgtc agacatctga cactggtcct tgttctttta ttttttatga aaatatctct
    26821 taatccagtc cgatttcttc tttgactact gccgcgattt tatcgacata gtaattaact
    26881 ttctcatcag taggcgcttc tgccatcact cgaagtaatg gttctgtacc ggaaggacga
    26941 actaggattc ttccttctga tcccatttca gcttctgcct cttcaatcac ttttttgatt
    27001 gccggaacat ccatcgcacc gtttttatca cttacgcgga tattcactaa tttttgcgga
    27061 tagattgtta cctctgctgc aagctctgat aatttcttac ctgtctgttt catgatattc
    27121 agtaactgaa ttcctgaaag catcccatct ccagttgtgt taaaatctaa gaaaatcatg
    27181 tgcccagact gctcgccgcc aaagttataa tcgtttttgc gcatttcttc tacaacgtaa
    27241 cgatcaccaa cttgtgtaac gacatctttc aaaccgattc cttccaccgc tttgtggaat
    27301 cctaagttac tcatgacagt cgtaacgatc gtatcttttt tcaatcgatt tttttctgcc
    27361 aagtatttag cacagataaa catgatttta tcaccgtcga caatgtttcc aagttcatcc
    27421 accgcaataa tacgatcgcc atcgccatcg aaagctagtc cagcatctgc tcctttttca
    27481 acgaccatct cagctaaacg ttctggatgg gtagagccta cgccgtcatt gatatttaac
    27541 ccgtctggat ttgtgcccat tgtataaaaa tctgtttcaa gatcggcaaa caaacgatta
    27601 acacttgttg aagttgctcc gtttgctgca tcgacacaaa tggtcaaacc ggaaaggtca
    27661 ccgttgatcg tttgtacaag gaattgagaa tatttcaata agccttctgg aaattcttct
    27721 acagttccca atccttcagc tgatggacga ggcaattcat ccacttcagc atctaataat
    27781 gcttcaattt ctagctcttg gtcatctact agtttaaaac catctgcacc gaaaaatttg
    27841 attccattat cttgtgctgg attatgagaa gcagaaatca tgacccctgc gcttgctttt
    27901 tgcaaacgtg tcagataagc aacacctggt gtagagatca cacctagctg gaacacttcg
    27961 atacccaccg ataaaagtcc tgaaatcagc gcttcttcta acatttgacc tgagattctt
    28021 gtatcccgcc ctactaacac gcgtgggcgt tttccttctt cttcatgctg gcttaggaca
    28081 taacctccgc accgtcccag cttaaatgca agttctggcg taagttcctt gttggcttca
    28141 ccgcgtacgc cgtctgttcc aaaatattta cccatagttg taaaattact cctttaactt
    28201 aattttctga ggaatttgtt ctgctcattt gtgttgattc gcttgtttct gatgtttctt
    28261 ctgtacttgt ggtcgagctg cttgtcccag attccgaact ggaagaaatg gttgttgtcg
    28321 atggttccgt tcctgaatca gcagatgaaa tacttctact cgtacttgta ttttcttgtg
    28381 tccgactgct cgtcgaagca gagctactgc ttgacgtttg caacacgggt gtaatctcca
    28441 cactaactga ttcgggcttg atacttaacc cctcttctac tggtatttca attttacgag
    28501 ttgttcgatg attgatacct gagacattga tcggtacagc aatactgtca ccgatcgatg
    28561 caagcaaatc gctagagccg cttacttctg cttccagttt tgaaaaactg aaatcatagc
    28621 tttctacccc ggcagctggt gttccttgct gtatcccata aagtcgtacc gacttgcttg
    28681 gcttgatcga atcagctctc actgttactt gcactggatc agagacgata cttaatactt
    28741 cccctgctgc attcagcgcc tgtattggga cctcactatt aatcccatca tctgtgacca
    28801 tcgaagcgtc cacatcagct actactcgtt tgatttctgc caatgttttg tcacctgtag
    28861 tgatagagac ttttttagga ctaacggaag tatcatcaag ttcatatcca gatggtgtga
    28921 acgttgaagg aatcaacgtt tccacatcga aatcttttat tacctttttc tcaaccgtta
    28981 ctgtaatagt cgatggttcg atctttgctg tgacagcact gctcaaattt tgaattttta
    29041 gcggaacttc atgtgttcct tcgcccagct tagtgatatc tgctgtgacg cggaacatcc
    29101 gtgtatcttc gtttgcctct gcattcaact gaactcggtt tgcactggaa agtttgacag
    29161 tcactgcatt ttcatatcca tggatatagt agcgctttga atcataagaa atcttgatcg
    29221 gcacccccgt aactgtttga ttatacgctt catttccaga aagcgtattt ggaacggact
    29281 ggccatttgc attgaagtac agcgcaatac tgaacagcaa tgcaataaaa ctgtaaagaa
    29341 tgcttgtccg tttctctttt gagatcattt gcgatcccct cccttagtca taccgtctag
    29401 gaaagattga atcacgtttt tcttgttttt tgttttctct tcagcaatta gttctctacg
    29461 caagattgcc aaatattctt gctgggataa gccagccatc agttcattat ctaacgtgat
    29521 gctgacgtcc ccggtttctt cagagacaac gatggtaatc gcgtcactga cttcgcttat
    29581 accgacagct gcccggtgtc gagtcccgaa ttctttaggg atcaacatac tttctgatag
    29641 aggaagatag gcgcttgcta ctgcgatctt tccttctttt acaataactg caccgtcatg
    29701 cagcggtgta ttaggaataa agatattgat cagcagctcc ccagtgatat ctgcatctaa
    29761 agcaatccct gtctcaatat actcatctaa tccagtatga cgttcgatag tgatcaacgc
    29821 accgattttt cgcttggaca tatattggat ggctttgtca aaagacaaga tcatcttctc
    29881 atcttcctgc tgttcgcttt ttgattgttt gaaaagcgaa ctgcgtccaa gatgttccag
    29941 tcctctccgg atttccggct gaaaaatcac gatagcagct atcaccccgt acgtaatgac
    30001 ttggttcatc agccaagaca aggtgtgcag accaatgatt tccgctacga ttcggataac
    30061 aataaaaata gctacacctt tcagaagctg aatcgctttt gtgcctcgca gcagttggat
    30121 cagtttgtat acaagatacc agacaaccag aatatcaagt atgttcacca aaaaattcag
    30181 agagaacaag tcaccggaaa acatttgctg ccaatagtcc aaattaaaaa gttccccgat
    30241 ttgaaatgac ataaaaagcc tctcttctct tttgagattt cctctctagt atatcatatg
    30301 ttttaatttt agaggctgat atgctcgtat ttttcttgag aaaaacgcat aattttcaag
    30361 aaaattgcaa gtatgcgttt tatttttctc tgcaataaat atttttaaac ttcagagaat
    30421 tattcattat attttttctg ttatttttcc tttttttctt ttgtagcttg ttccattttt
    30481 ttacgtcgtt ctgctctggc agctcttcta gcagctcttc tttcttctgc ttcaaggtcc
    30541 gtcgtttttt cttttctagt cttaacgggt acatcgatag attcctctgg ttcaagtcgt
    30601 ttcttgattt tttctaattc tcccgtcagt gcagcatagt tgtaaaaacg tgttttagcg
    30661 tcgtggacag tcttttcaaa taaagctttt gcaagatttg gatttgcgtt atctagcgag
    30721 gaaaagcgaa cttgtttcct catgaaatca ggcatagcag agaaatcagg ttttttgtag
    30781 tccaaagtca ttgggttctt gtttttttcg accattgtag ggttatatcg atataatgac
    30841 caataaccag aagaaacagc ttcttttgct tctttaagcg attctttcat tccgccaagc
    30901 aagccatgtg tgatacaagg cgtataggca ataatcagag aaggaccagg atacttttct
    30961 gcttcttcaa atgccttgat cgtttgcatt tgattggcac ctgaagcaat ttgcgccaca
    31021 taaacatttc catatgtcat cgccatcatt cctagatctt tctttgaagc atactttcca
    31081 ctagccgaaa atttagcgat tgccgaagct ggtgttgctt ttgaggtttg accgccagta
    31141 ttggaataga cttcgttatc caaaacaagg atattcacgt ctgctccgct ggcaagtaca
    31201 tgatcgatcc cgccaaaacc aatgtcgtaa gcccatccgt ctccaccaat catccattgg
    31261 ctgtttttta cgaatagatc ctttctcttg tatatatcct ccagttcagg gaaaattgat
    31321 cgttcgcttt cgatcgcagc ttgcagtttt gctgctcttt gctgcgttcc ttcactctca
    31381 aacatgtgta ctaaccagtc ttccatcaat tctcgtagtt cttctgatac ttttggcaat
    31441 acttctttga ttgaacttgc caaagcctcg cgacgagtct gtgccgctag aagcatacca
    31501 taaccaaatt cagcattatc ttccaataaa gaatttgacc atgcaggccc ttgaccacat
    31561 tcattggtcg tataaggaga agcaggagaa gcaccgcccc aaatagaaga acagcctgtc
    31621 gcattcgcga tcatcattcg atcaccgaac atttgtgtca ataattttac ataaggtgtt
    31681 tcgccacagc ctgagcaagc gcccgagaat tcaagaagcg gttgattgaa ttgtgtagac
    31741 aagacagtat tctttccttt gactggattt gctttttgtc ttaatgtcat tgcaaaagcc
    31801 caattcacag cttgttcttt ctgttcttca taaggcttca tcactaaagc tttttctttt
    31861 gcgggacaag cctctacaca cagaccacaa cctgtacagt cttccactga aacttggata
    31921 cggtatttta cgccgtctgc gcctcgcatt tctctcacga taaatccttc tggcgcttct
    31981 tccatttcct cttcatctgc taaaaatgga cggatggcag catgaggaca aacaaaggca
    32041 cattcattac acatcgtaca gcgatcacta atccactcag gtacttctaa agcgacgccc
    32101 cgcttttcca tagaagccgt tcctaaaggc atttcgccag ttgtcattcc atttgctaca
    32161 agtgtaccaa cagagagttg atttccttcc tgtgcattca ctggttcaag tatttcttgt
    32221 atataaccag tgttcctttt attgattttt tcaggcatct ccaacgttct ccaatggtct
    32281 gggatcgcga cttgatgaag cagttcggtc gtccgatcga ttgcttgtat gtttttttct
    32341 acgatagcca ttgatttatg tccatagctt ttcagggctt cttcttttag cagcggaagc
    32401 acctcatcaa aaggaatgat gtcagctaat ttaaaaaaag ccgtttccat tgccgtattg
    32461 atccggcgtc ccaaaccgac ttcttgggcc aatctcatgg cattgatcgt atagaatcta
    32521 atgttatttt cagccaagta acgttttaat tttaagggca gatgcgtttc taattgttcg
    32581 tcgctccaaa gagtattcaa taaaaaaatg ccacctggct tcaatccttt gaccaagtca
    32641 tagctatgca ggtaagctgg tgtgtgacag gcaacaaggt ccgcatgctc gacaagatac
    32701 gctgaacgta tcggcgtatc tccaaaacga agatgagaga cagtcagacc gccagatttt
    32761 ttcgaatcat aataaaaata gccttgcgca tatttatcgg tgtgatctcc aataatcttg
    32821 atagctgatt tatttgctcc aactgttccg tcagatccaa atccccaaaa cttggcctgg
    32881 aatgtttgcg gttctgtcaa atccagactt tcctttggtt ccaaagattg ataagtcaca
    32941 tcatccacga tccctatggt aaaccgtttt ttgcgtatgc ttgggtcttt tttcaattca
    33001 tcaaaaactg ctgagatttg gtcgggagtc acatctttag accccaatcc gtagcgacca
    33061 ccaataacag ccgggcgaag ttcagaatca tataacgcac tttgaacgtc taataagaga
    33121 ggttctccac ctgcccctgg ttctttactt cgatcaagaa cagcaattgt ttttactgta
    33181 tgaggcaact tttgcacgaa tgtttccact ggaaaaggac gataaagatg gatattcaaa
    33241 aaacctgttt tccgtccgtt tgcattcaag taatcaatcg tttgttcaat cgtctgtgca
    33301 actgatccca tagaaacaat gacttcttcc gcatcgtctg ctccgtaata agtcaccaag
    33361 tcataagaag ttcctcgtaa tcggttgatc tctttcatat atttttgaac gatttcaggt
    33421 aatttatcat aataggggtt gatcgtttcc cgttgttgaa aatggatgtc tggattctgg
    33481 ttcgtaccac taacagtcgg atgattggga ttcatccctc gtttacgaaa tgaagctaat
    33541 ttctcctgat tgatcagagg agctaattct tcatattcca aaacttcgat tttttgaatc
    33601 tcgtgactcg tacggaatcc atcaaagaaa ttcaaaaaag ggacttctcc ttcaatagaa
    33661 gcaagatggg caacaggtgc taaatccatc acttcttgta cactgctttc agctaacata
    33721 gcaaatcctg tttgtcttgc agccatcacg tctccgtgat ctccgaaaat attcaacgca
    33781 tttgtcgtca ctgcccgact agcaacgtgg aaaacacttg gcagtagttc cccagcaatc
    33841 ttgtacatat tcggtatcat caaaagcagc ccttgtgaag cggtatatgt agttgtcagt
    33901 gcaccagttt tcaatgatcc atggaccaca ccagctgctc ctgcttcgga ttgcatttct
    33961 actactttta ctggttgacc gaatacgttt tcttttcctg ctgctgacca ttgatctact
    34021 aattcagcca tggtcgaact gggtgttatt ggatatattg cagcaatctc ggtaaatgca
    34081 taagaaatat atgcggctgc cgtattgcca tccattgttt tcatttttcg catgattcat
    34141 catccttgtt catattctct cttattatct gttccattat aacgtaaaac atgccatttt
    34201 tttcttttat ttataaaaat attttacatg tatctatttg aattggtcat ctaattttta
    34261 gacaaacagt ctaaatcgtt gccttttttt gtcagacatg tataaactat aagcgaaaga
    34321 ggtgttttaa catggatata tcagtgattg atgcaacgaa aacaaataca caaaaaggaa
    34381 tcctttatgg ttcatcgaat gctcctaaaa aaatggtcga attcatcaac ttagcttgtc
    34441 cgtattgccg acaatggttt gaagaatctt atgagctatt agaagaagca gtccaaagcg
    34501 ggcaattgca gcgagtgatc aaattgttcg ataaagaaaa agaaagctta cagcgaggga
    34561 acgtgatgca ccggtatctc acaatcagcg atggccaaaa agcgatcaaa gagatcaaac
    34621 aaatattcga tactcaagac gagtggaaac atctttctct ccaagaagta gcggattttg
    34681 cagtagataa actaaagctg acagaacaaa aagacgaaca gctgagccaa gctgtcatca
    34741 acgaagctga gcaagcacat attcgatttg ttccaactgt catcttgggt aaagaaatct
    34801 ttgatgaatc tatttcaata gaggaattaa aagaattgat ccaaacaaaa taaaaaaata
    34861 aggagcgtgg gacaaaagta aagaacactt ttgtctcacg ctctaaacac gtataaacgg
    34921 cggaagcaga agcaactcct tctt
//
LOCUS       DARBET010000023        34699 bp    DNA     linear   BCT 07-NOV-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4027
            SAMEA114569841-rid20112183.denovo.023, whole genome shotgun
            sequence.
ACCESSION   DARBET010000023 DARBET010000000
VERSION     DARBET010000023.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA114569841
            Sequence Read Archive: ERR12203666
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 34699)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 34699)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001972618.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001972618.1
            Genome Coverage       :: 132x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/07/2023 13:18:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,041
            CDSs (total)                      :: 2,975
            Genes (coding)                    :: 2,908
            CDSs (with protein)               :: 2,908
            Genes (RNA)                       :: 66
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 67
            CDSs (without protein)            :: 67
            Pseudo Genes (ambiguous residues) :: 0 of 67
            Pseudo Genes (frameshifted)       :: 26 of 67
            Pseudo Genes (incomplete)         :: 28 of 67
            Pseudo Genes (internal stop)      :: 20 of 67
            Pseudo Genes (multiple problems)  :: 6 of 67
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..34699
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA114569841-rid20112183.denovo.023"
                     /isolate="VRE4027"
                     /db_xref="taxon:1352"
                     /country="Denmark"
     gene            654..1343
                     /locus_tag="SCJ86_001230"
     CDS             654..1343
                     /locus_tag="SCJ86_001230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002411958.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HEG1168804.1"
                     /translation="MKILIADDDKEIVELLSIYVHNEGYEAVKAYDGKEALSKIRTIP
                     DIELLILDIMMPEMDGMQVVKELRKESQIPIIMLTAKTTDMDKIKGLVAGADDYVTKP
                     FNPLEVMARVKSLLRRTKMQIAPDQPDVLEVNALIINKDSHEVKTIDGKEIQLTALEF
                     GILYLLASHPNRVFSADEIFERVWKQESIVSAKTVMVHVSHLRDKIEEATGGEKVIQT
                     VWGVGYKIDAR"
     gene            1330..2538
                     /locus_tag="SCJ86_001231"
     CDS             1330..2538
                     /locus_tag="SCJ86_001231"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330803.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing histidine kinase"
                     /protein_id="HEG1168805.1"
                     /translation="MTQDSKRATEKRRRINLTSKEISELLAEGIITIILLLLLNVSIL
                     VVISSVINSSPSLTNAIWDSKNIFAERLNTDLFWNGRNFIIPFFFLLDIGVLYWRLIR
                     RYRQMQLRHIISELHYIANGNYDHRIPFELSGDLSRVVTSINGLVDSTVAAIEDERKI
                     EKSKDELITNVSHDIRTPLTSIIGYLGLIEDGQYHSEEDLLKYTHTAYIKAKQMKSLV
                     DDLFEYTKVRQPAVPVNFSAFDMIQLIEQLAADFELEASKKNIQILVQSKVDSLIMDG
                     DTEKLVRVFNNLLTNALKYGKGATKIVIEVERIGSEVVATVKNNGAMIPQQAIDNLFD
                     RFYRVEESRSQATGGTGLGLAIAQSIVALHGGYIYAKSDKQWTSFIMHLPIKKNEKLP
                     IDTQEVIDES"
     gene            2832..4103
                     /gene="serS"
                     /locus_tag="SCJ86_001232"
     CDS             2832..4103
                     /gene="serS"
                     /locus_tag="SCJ86_001232"
                     /EC_number="6.1.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338634.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine--tRNA ligase"
                     /protein_id="HEG1168806.1"
                     /translation="MLDVKMMRQNIEEVQEKLMTRGVKKETLAEFMELDENRRKLLVK
                     SEELKKYRNDVSAEIAQLKRAKEDASAKIAEMKEVGGNIKALDTEIAEVDEKLKQIAT
                     TLPNLPHDSVPVGTDEEDNVEIRRWSEPRTFAFEPKPHWEIAENLDILDFERGAKVAG
                     SRFVYYKGLGARLERAVYNFMLDQHIYEHGYTEMITPYIVNSKAMFGTGQFPKFKEDV
                     FQLENTDLTLIPTAEVPLTNYYNDEILDGADLPIYFTALSPAFRSEAGSAGRDTRGLI
                     RLHQFNKVEMVKFSDVEHSYEELEKMTADAEDILKKLHLPYRVMALSTGDMGFSAAKT
                     YDLEVWIPAQDTYREISSCSNCEDFQARRAMIRYRDNDGKIHYTHTLNGSGLAVGRTV
                     AAILENYQNEDGSVTVPEALVPYMGNLKVIK"
     gene            complement(4652..6136)
                     /gene="guaB"
                     /locus_tag="SCJ86_001233"
     CDS             complement(4652..6136)
                     /gene="guaB"
                     /locus_tag="SCJ86_001233"
                     /EC_number="1.1.1.205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875762.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IMP dehydrogenase"
                     /protein_id="HEG1168807.1"
                     /translation="MSNWETKFTKKGFTFDDVLLIPAESHVLPNEVDMSVQLAKNIKL
                     NIPIISASMDTVTDSKMAIAMARQGGLGVIHKNMTISQQADEVRKVKRSESGVIIDPF
                     FLTPQHLVADAEELMSKYRISGVPIVETLENRKLVGIITNRDMRFVTDYHMPIADVMT
                     KDNLVTAPVGTSLKDAEKILQKHKIEKLPIVDNEGRLSGLITIKDIEKVIEFPNAAKD
                     EHGRLLVAAAVGVTSDTFERANALLEAGADAIIIDTAHGHSAGVIRKIQEIRSTFADA
                     TLIAGNVATAEATKALYDAGVDVVKVGIGPGSICTTRVVAGVGVPQLTAIYDAASVAR
                     QYGKAIIADGGIKYSGDIVKALAAGGHAVMLGSMLAGTDESPGEFEIFQGRRFKTYRG
                     MGSLGAMEKGSKDRYFQGSVNEANKLVPEGIEGRVAYKGSVADIIFQMIGGLRSGMGY
                     VGAANLQQLRDEAQFIQMSGSGLKESHPHDVQITKEAPNYSVES"
     gene            complement(6641..7336)
                     /locus_tag="SCJ86_001234"
     CDS             complement(6641..7336)
                     /locus_tag="SCJ86_001234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296943.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1129 domain-containing protein"
                     /protein_id="HEG1168808.1"
                     /translation="MEPEQLRAIVKENNSLETKLTKRNQQYIFDLKKSLEAANLSEEE
                     KVKALHDILPTLVAEQKGGRTARQLFGTVSERTEAIINKPAEVKNSSTPLLMWLDNTL
                     LLLGLLSIMIAITGLFSRGTTPAYGFSTLVLGSAIGGWVFYMMYKYIYQYEYPGADRS
                     KKPGMGKTLLILGGTTLLWVVLFSATTLLPPVINPVLDPVVVIIIGATALAIRYYLKK
                     KYGIIGSLSVPRR"
     gene            complement(7426..8526)
                     /gene="ychF"
                     /locus_tag="SCJ86_001235"
     CDS             complement(7426..8526)
                     /gene="ychF"
                     /locus_tag="SCJ86_001235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="redox-regulated ATPase YchF"
                     /protein_id="HEG1168809.1"
                     /translation="MALTAGIVGLPNVGKSTLFNAITKAGAEAANYPFATIDPNVGMV
                     EVPDWRLQRLTELVHPKKTVPTTFEFTDIAGIVKGASKGEGLGNQFLSHIRQVDAICH
                     VVRCFDDDNITHVEGRVDPLEDIDTINLELVLADLESINKRYTRVAKVAKTKDKDAVA
                     ELAVLDKIKPVLEEGKSARTIEFTEEEQKIVKSLFLLTTKPILYVANVSEDDVADADA
                     NHYVQEVRNFAAEENAEVIVVSARAEEEIAELDEEDKAEFLEALGIEESGLDQLIRAA
                     YDLLGLATYFTAGEQEVRAWTFKKGIKAPQAAGIIHSDFERGFIRAETVSFEDLDHYG
                     NMHAAKEAGRVRLEGKDYVVQDGDVMLFRFNV"
     gene            complement(8535..8726)
                     /locus_tag="SCJ86_001236"
     CDS             complement(8535..8726)
                     /locus_tag="SCJ86_001236"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF951 domain-containing protein"
                     /protein_id="HEG1168810.1"
                     /translation="MYDLHDIVEMKKPHACQTNRWEVIRMGADIKIRCMNCGHIVMMT
                     RRDFEKKLKKVIEKNAEKE"
     gene            complement(8747..9637)
                     /locus_tag="SCJ86_001237"
     CDS             complement(8747..9637)
                     /locus_tag="SCJ86_001237"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345773.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ParB/RepB/Spo0J family partition protein"
                     /protein_id="HEG1168811.1"
                     /translation="MKKSKGLGRGIDALFQDLENLETVDVKDDTVVQLPLNELRPNPY
                     QPRKTFEEVSLQELANSIEQSGVFQPIIVRRSAVKGYEIIAGERRFRASKLAEKETIP
                     AIVRDFDEEAMMQVAVLENLQREDLNPLEEAEAYEMLMKNLKLTQAEVASRLGKSRPY
                     IANYLRLLSLPDLVKEMVQDQRLSMGQARTLLGLKNKTLILKLANRAVTENLTVRQLE
                     QLVSELNQNDGKEKKKIPRLAKEKPYYIRESEDRLMDKFGTSVAIQEKNGKGKIEIEY
                     LSQKDLTRILDILNIQFDEE"
     gene            complement(9624..10391)
                     /locus_tag="SCJ86_001238"
     CDS             complement(9624..10391)
                     /locus_tag="SCJ86_001238"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010750889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ParA family protein"
                     /protein_id="HEG1168812.1"
                     /translation="MAQIISVANQKGGVGKTTTTVNLGACLASLGKKVLLVDMDAQGN
                     ATSGVGIRKPDVTRDIYDVLVNELPIDEATLITEHENLSIVPATLQLAGAEIELTSMM
                     ARESRLKGSLAEVSSQYDYILIDCPPSLGHLTINSFTASDSILIPVQCEYYALEGLSQ
                     LLNTVRLVQKHFNPELEIEGVLLTMYDARTNLGNEVVEEVRKYFREKVYETIIPRNIR
                     LSEAPSHGKPIIDYDPRSRGAEVYQALAKEVVSREEK"
     gene            complement(10403..11116)
                     /gene="rsmG"
                     /locus_tag="SCJ86_001239"
     CDS             complement(10403..11116)
                     /gene="rsmG"
                     /locus_tag="SCJ86_001239"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342734.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (guanine(527)-N(7))-methyltransferase
                     RsmG"
                     /protein_id="HEG1168813.1"
                     /translation="MIPEEFRQSLAEKGIELTDHQMNQFAIYYRTLVEWNEKMNLTAI
                     TDEKDVYLKHFYDSLTLAFEVDFTQPLSLCDVGSGAGFPSIPLKIAFPELQITIVDSL
                     NKRITFLSELTRLLGIDASLYHDRAETFGQNPHFRASFDLVTARAVARLNVLAELCLP
                     LAKQEGYFYALKAAKSEEELTEAKQAIALLGGKFLKEEAVTLPNVSDQRHIIVIQKKK
                     PTPKKYPRKPGLPNKQPIK"
     gene            complement(11348..13249)
                     /gene="mnmG"
                     /locus_tag="SCJ86_001240"
     CDS             complement(11348..13249)
                     /gene="mnmG"
                     /locus_tag="SCJ86_001240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328632.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA uridine-5-carboxymethylaminomethyl(34)
                     synthesis enzyme MnmG"
                     /protein_id="HEG1168814.1"
                     /translation="MEHYEADAYDVIVVGAGHAGSEAALAAARMGSKTLLLTINLDMV
                     AFMPCNPSVGGPAKGVVVREIDALGGEMGKNIDKTYIQMRMLNTGKGPAVRALRAQAD
                     KHAYSTEMKHTIEKEPNLTLRQGIVEELIVEDGVCHGVVTSTGASYRAKAVVITAGTA
                     LRGEIIIGELKYSSGPNNSQPSIGLANHLKELGFAIERFKTGTPPRVKSSTIDYSVTE
                     EQPGDKEPNHFSFSTPDSAYKLDQESCWLTYTGETTHQIIRDNLHRAPMFTGIVEGVG
                     ARYCPSIEDKIVRFADKPRHQLFLEPEGLNTEEVYVQGLSTSLPEDVQVNMLHSIAGL
                     EKVEMMRTGYAIEYDVVEPHQLRPTLETKVVENLYTAGQTNGTSGYEEAAGQGLIAGI
                     NAALKVQGKEPLVIKRSDGYIGVMIDDLVTKGTNEPYRLLTSRAEYRLLLRHDNADLR
                     LTEMGHRVGLVKEDQYKNYLKKKEAVEQEIKRLMNVRIKPTEEVQDFLATINAAPLKD
                     GVLASDFLRRPEITYAELLRFIPENEELTRKEIEQVEIQIKYEGYIKKAMEKVEKLKR
                     MEAKKIPENIDYAAINGLATEAKQKLQKIQPETIAQASRISGVNPADLSILMVYIEQG
                     KIAKVSEPA"
     gene            complement(13266..14663)
                     /gene="mnmE"
                     /locus_tag="SCJ86_001241"
     CDS             complement(13266..14663)
                     /gene="mnmE"
                     /locus_tag="SCJ86_001241"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA uridine-5-carboxymethylaminomethyl(34)
                     synthesis GTPase MnmE"
                     /protein_id="HEG1168815.1"
                     /translation="MAEITLEFDTIAAISTPPGEGAISIVRLSGDQAVQLADKVYQSG
                     NKRLSEVPSHTIHYGHIVDPKSNQLVDEVMVSVMRAPKTFTREDVVEINCHGGIVVVN
                     QILQLLLREGARLAEPGEFTKRAFLNGRVDLSQAEAVMDLIRAKTDKAMGLALNQLDG
                     NLSALIRSLRQEILETLAQVEVNIDYPEYDDVEELTTKLLLEKAQMIQQRIQALLATS
                     KQGKVLREGLSTAIIGRPNVGKSSLLNHLLREEKAIVTDIAGTTRDVIEEYVNVRGVP
                     LKLIDTAGIRETEDVVERIGVERSRKALAEADLILLVLNQSEPLTAEDEQLLEATSGL
                     KRIILLNKTDLPAQLEQEKLKKLIENEPVFSISVAKNDGLDRLESAISDLFFSGETGE
                     RDATYVSNTRHIALLEKASLSLEEVIAGIDSGMPVDLVQIDMTRCWDYLGEVVGDSVQ
                     DELITQLFSQFCLGK"
     gene            14962..15345
                     /locus_tag="SCJ86_001242"
     CDS             14962..15345
                     /locus_tag="SCJ86_001242"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375983.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEG1168816.1"
                     /translation="MVALSKLYTFERRKYVSPFRSAALEEESDPRINDVVLSTGNINQ
                     KYIVRDIVFVVECLEADLFDPSFPKDDLFLTTKRKLKTKVLEYGANAVINCHFEYQQK
                     KRDGKYFLELFAYGTVIQFIQTTIG"
     gene            complement(15513..16271)
                     /locus_tag="SCJ86_001243"
     CDS             complement(15513..16271)
                     /locus_tag="SCJ86_001243"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Jag N-terminal domain-containing protein"
                     /protein_id="HEG1168817.1"
                     /translation="MPIYEAATIDEAVQLGLADLGLAKDQVEIEILEEGKKGFLGMGR
                     KNARLSIQPSISEKVSETVEQTVEEILEEEPTAEIEVVNETILEEKTEEVSELGDEAA
                     LTELAVYLTEISKQLDAPALVKMERESNNMVVFHLETQKQGILIGKHGKTLNALQYLA
                     QVFIHRLARNKLSVVVNVGNYREKRQEIIQRLADRTAEKVKRTGQPVFLEPMPAFERK
                     QIHAALSKDEHVKTHSEGEEPYRYLVVEPAKKYY"
     gene            complement(16289..17107)
                     /locus_tag="SCJ86_001244"
     CDS             complement(16289..17107)
                     /locus_tag="SCJ86_001244"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332030.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane protein insertase YidC"
                     /protein_id="HEG1168818.1"
                     /translation="MKKYKRLLLMAGIVSLVIVLSGCGTGEVSAQSTGIWDRYIVYYF
                     AEAIKALSFGNAGIGIILFTLIIRVILLPLMHFQTKSMRKTQELQPKLKALQQQYSSR
                     DPETQSKLREEQQRLYAENGVNPYAGCLPLLVQMPILMALWQSISRVPELKQGHFLWL
                     NLGNADPTFILPILAAIFTFASTYLSSMSQIESNASMKIMNFVMPVMILVMGVNLASG
                     LSLYWVVSNAFQVVQTLLINNPFKIRKEREEEARRIRERERALKKAMNPKKKRK"
     gene            complement(17104..17460)
                     /gene="rnpA"
                     /locus_tag="SCJ86_001245"
     CDS             complement(17104..17460)
                     /gene="rnpA"
                     /locus_tag="SCJ86_001245"
                     /EC_number="3.1.26.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010720505.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease P protein component"
                     /protein_id="HEG1168819.1"
                     /translation="MRKAYRVKKEREFQAVFHTGDSFANRKFVVYRLEKPGQPHFRVG
                     LSVGKKVGNAVNRNEVKRKIRAAVYSIKEEIDPELDFIVIARPSVKGLTYDEVRSNLV
                     HVLKLAKIIDREEKSK"
     gene            complement(17578..17712)
                     /gene="rpmH"
                     /locus_tag="SCJ86_001246"
     CDS             complement(17578..17712)
                     /gene="rpmH"
                     /locus_tag="SCJ86_001246"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003397591.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L34"
                     /protein_id="HEG1168820.1"
                     /translation="MKRTYQPNKRKRQKVHGFRKRMSTKNGRRVLASRRRKGRKVLSA
                     "
     gene            18235..19569
                     /gene="dnaA"
                     /locus_tag="SCJ86_001247"
     CDS             18235..19569
                     /gene="dnaA"
                     /locus_tag="SCJ86_001247"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010720504.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chromosomal replication initiator protein DnaA"
                     /protein_id="HEG1168821.1"
                     /translation="MVSLDALWNELKATYQKDLSPASYNTWIETANPRTLDQNQLVVE
                     VPSKIHKEYWEKNLATKIVEVGYMLSGNEIIPRFITGEEAEQEEVIEEKNPKVVAPSP
                     LKKAMLNPKYTFDTFVIGKGNQMAHAAALVVAEDPGSIYNPLFFYGGVGLGKTHLMHA
                     IGHQMLQSQPNAKVKYVSSETFANDFINSIQNKTAEEFRQEYRNVDLLLVDDIQFFAE
                     KEATQEEFFHTFNALYNEGKQIVLTSDRLPNEIPKLQERLVSRFAWGLSVDITPPDLE
                     TRTAILRKKAAAERLEIPDDTLSYIAGQIDSNIRELEGALVRVQAFAAMNSEDISTSL
                     AADALKTLKSGKGHPQLSILQIQEEVAKYYHIQLKDLKGKKRVKSIVVPRQIAMYLAR
                     ELTDNSLPKIGAEFGGKDHTTVIHAHEKIQQLMDSSVSMQNEVSEIKNFLLN"
     gene            19767..20897
                     /gene="dnaN"
                     /locus_tag="SCJ86_001248"
     CDS             19767..20897
                     /gene="dnaN"
                     /locus_tag="SCJ86_001248"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit beta"
                     /protein_id="HEG1168822.1"
                     /translation="MKVTLNRASFMQELQTVQRAISSKTTIPILTGVKITLTQEGLTL
                     TGSNADISIETFLSVENEKANMQIESTGSIVLQARFFSEIIRRLPEETFTLEVLENKQ
                     VAITSGKANFIVNGLDADNYPHLPVVESHNQMKLPVHVLTKLINETVFAVSQHESRPI
                     LTGVHFILSDNSLLAVATDSHRLSQRVIPVEQAADHFDIVIPGKSLIELSRSLTNEEE
                     IVEISIMENQVLFKTETMYFYSRLLEGNYPDTNRLIPSSFNTEVEFSVPSFLAAIERA
                     SLLSHEGRNNIVRLSIRPDAVVLYGNSPEIGKVEESLSYTASSGDPLDISFNPDYMKA
                     ALRAFGDMSIKVKFISAIRPFTLEPTEDGVQFIQLITPVRTN"
     gene            21126..21368
                     /gene="yaaA"
                     /locus_tag="SCJ86_001249"
     CDS             21126..21368
                     /gene="yaaA"
                     /locus_tag="SCJ86_001249"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S4 domain-containing protein YaaA"
                     /protein_id="HEG1168823.1"
                     /translation="MKQQVILNTEFMTLGQMLKEVTVIGSGGQAKWYLAENTVLVDGE
                     PENRRGRKLYPGMMVEVPEVGTFFMVKKGNDDNEAE"
     gene            21355..22479
                     /gene="recF"
                     /locus_tag="SCJ86_001250"
     CDS             21355..22479
                     /gene="recF"
                     /locus_tag="SCJ86_001250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309899.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA replication/repair protein RecF"
                     /protein_id="HEG1168824.1"
                     /translation="MRLNKLYLKNYRNYEELNIEFSKNLVIFLGENAQGKTNLLESIY
                     VLAMTRSHRTSSEQELIGWTDDQAMIQGEITKGSSTLPLEILLSKKGRKTKINHIEQK
                     KLSTYVGQMNVILFAPEDLSLVKGSPQTRRKFLDMELGQINPIYLYDLVQYQAILKQR
                     NQYLKQLNEKKQTDLLYLDILSEQLAAFGSKVLKARAQFVKRLEFWANSLHQQITHQK
                     EQLEIEYLTAVDSLETHTQEQIQEQFLALLNQNKKKDLFRGTTTVGPHRDDLSFFINQ
                     KNVQTYGSQGQQRTTALSVKLAEIDLIKEETGEYPILLLDDVMSELDDNRQLHLLETI
                     EGKVQTFLTTTTLDHVKNKMTVEPEIFYVQQGKLKGEETE"
     gene            22476..24422
                     /gene="gyrB"
                     /locus_tag="SCJ86_001251"
     CDS             22476..24422
                     /gene="gyrB"
                     /locus_tag="SCJ86_001251"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003129249.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase (ATP-hydrolyzing) subunit B"
                     /protein_id="HEG1168825.1"
                     /translation="MTEERSLVERAKEYDASQIQVLEGLEAVRKRPGMYIGSTSSEGL
                     HHLVWEIVDNSIDEVLAGFATKIHVIIEKDNSITVIDDGRGIPVDIQAKTGRPAVETV
                     FTVLHAGGKFGGGGYKVSGGLHGVGSSVVNALSTQLDVKVYKEGNVYYQEYRRGAVVD
                     DLKIIEQTDRHGTTVHFTPDPEIFTETTEFDFSKLATRIRELAFLNRGMRISIEDKRE
                     EEPVINEYHYDGGIKSYVEYLNANKTVIFPEPVYLEGEQQDIAVEVSMQYTDGYHSNI
                     MSFANNIHTYEGGTHESGFKTALTRVINDYARKQKLMKENDDNLTGEDVREGLTAVIS
                     IKHPDPQFEGQTKTKLGNSEVRTVTDRLFSEHFMKFLLENPSVGRQIVEKGLLASRAR
                     LAAKRAREVTRRKGALEISNLPGKLADCSSNDPEKCELFIVEGDSAGGSAKQGRNREF
                     QAILPIRGKILNVEKASMDKILANEEIRSLFTAMGTGFGEDFDVSKARYHKLVIMTDA
                     DVDGAHIRTLLLTLFYRYMRPIVEAGYVYIAQPPLYGVKQGKNITYVQPGKNAEEELK
                     QVVASLPASPKPSVQRYKGLGEMDDHQLWETTMDPSNRMMARVSVDDAIAADQIFEML
                     MGDRVEPRRAFIEENAHYVKNLDI"
     gene            24442..26913
                     /gene="gyrA"
                     /locus_tag="SCJ86_001252"
     CDS             24442..26913
                     /gene="gyrA"
                     /locus_tag="SCJ86_001252"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631904.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA gyrase subunit A"
                     /protein_id="HEG1168826.1"
                     /translation="MSEEIRENIHDVNLTSEMKDSFIDYAMSVIVARALPDVRDGLKP
                     VHRRILYGMNELGVTPDKAHKKSARIVGDVMGKYHPHGDYAIYESMVRMAQPFSYRYM
                     LVDGHGNFGSVDGDGAAAMRYTEARMSKIATEMLRDINKNTVDFQSNYDDTEKEPVVL
                     PARFPNLLVNGTTGIAVGMATNIPPHNLSEVVAAIDLLMDNPDVTTNELMEVLPGPDF
                     PTGGLVMGKSGIRRAYETGKGSITVRAKVEITEMPNGKERIIVTELPYMVNKAKLIER
                     ISELHRDKRIEGITDLRDESSREGMRIVIDIRRDASASVILNNLYKLTSLQNSFGFNM
                     LAIEKGVPKVLSLKQILENYIEHQREVITRRTVFEKEKAEARAHILEGLRIALDHIDE
                     IIAIIRGSKSDDEAKATMIERFDLSDRQSQAILDMRLRRLTGLEREKIENEYQELLKL
                     IEDLTDILARPERVTEIIKNELADLNQRFGDKRRTELLVGEVLSLEDEDLIEEEEIVI
                     TLTNNGYIKRLANNEFRAQRRGGRGVQGMGIHNDDFVKNLVSCSTHDTLLFFTNNGKV
                     YRAKGYEIPEYGRTAKGIPIINLLGIDSSESIQAIIAVTGEAEEGHYLFFTTRQGTVK
                     RTSVTAFANIRSNGLIAIGLKEDDELVNVLLTDGQSNIIIGTHNGYSVTFKEEAVRDM
                     GRTASGVRGIRLREEDYVVGAALMKEDQEVLVITEKGYGKRTKVSEYPVKGRGGKGIK
                     TANITEKNGPLAGLTTVSGDEDIMLITDKGVIIRFNVSTVSQTGRSTLGVRLMKMEAD
                     TKVVTMAAVEPEAAEQTVEEQGSEE"
     gene            27149..27448
                     /locus_tag="SCJ86_001253"
     CDS             27149..27448
                     /locus_tag="SCJ86_001253"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005226363.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S6"
                     /protein_id="HEG1168827.1"
                     /translation="MENTKYEIMYIIRPNIDEEAKTALIERFDAILKDNGAEVIESKD
                     WEKRRFAYEMNGYREGIYHIVKVSSPSSANAVNEFDRLAKINDDIIRHMIVKEEA"
     gene            27498..28010
                     /gene="ssb"
                     /locus_tag="SCJ86_001254"
     CDS             27498..28010
                     /gene="ssb"
                     /locus_tag="SCJ86_001254"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010750861.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded DNA-binding protein"
                     /protein_id="HEG1168828.1"
                     /translation="MINNVVLVGRLTKDPDLRYTASGTAVATFTLAVNRNFTNQSGNR
                     EADFINCVIWRKSAETLANYARKGTLLGVTGRIQTRSYDNQQGQRVYVTEVVADNFQL
                     LESRSASENRQQSGGFQSSGQSAGGFGGNNNSNQTSQSSNGMPDFDRDTSDPFGSSST
                     IDISDDDLPF"
     gene            28036..28272
                     /locus_tag="SCJ86_001255"
     CDS             28036..28272
                     /locus_tag="SCJ86_001255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288370.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S18"
                     /protein_id="HEG1168829.1"
                     /translation="MAQQRRGGRKRRKVDYIAANHIEYIDYKDIELLKRFISERGKIL
                     PRRVTGTGAKNQRKITVAIKRARIMGLLPFVSED"
     gene            28415..30388
                     /locus_tag="SCJ86_001256"
     CDS             28415..30388
                     /locus_tag="SCJ86_001256"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629811.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DHH family phosphoesterase"
                     /protein_id="HEG1168830.1"
                     /translation="MENKNNHLSPKPLFLILFILIEVLVVLILPFRFVSASVILLMNL
                     WGVRYWLKMKRWLILNKREKIQQASTVAETNLAYVSEVMPVGVIAYQPSSQKIEWLNP
                     LALKIKDQSGLTEEELLDTFFTAKEKKKNQVYLADKTFQFDMDTVKGVVYFNDITQNQ
                     LLKLHQTNSQPVIGILSIDNYADAIDKLDDKEISYLNSFVTTLVSDWMNEYHVFFKRI
                     NAERFFFVAQMIDLKKMMDKKFDLLDRFRAEAEKQSSPITLSMGIAYGSRTLDEIGGE
                     AQNNLDVALVRGGDQVVVKEVKEGAKPVYYGGKSAKNVKRTRVRSRAMSTALRRIFDE
                     TGDVFIMGHKFPDMDALGSAFGVSCLARFNQKKAYIVINENEIIPDVERCLEEIHKHP
                     ELEMQLISPERAIELKKEEDLLVMVDYHKPSLSISQGLYEQFDKIVIIDHHRRGEEFP
                     SKPLLTYIESSASSASELVSELIQYQSSKTNRLDRFEATLLLAGMVVDTNSFNVRTTA
                     RTFDVASYLRSCGADASLVQYLLSSDLATYLEISNLIAGSDYVTSDIVVAAGTDEKEY
                     NSVIAAKTADTLLSMININAAFVITKRTDGLIGISARSTGTINVQIIMESLGGGGHFT
                     NAATQLENTTVEETKQKLFAAIKENMNQIYDKE"
     gene            30394..30846
                     /gene="rplI"
                     /locus_tag="SCJ86_001257"
     CDS             30394..30846
                     /gene="rplI"
                     /locus_tag="SCJ86_001257"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002371936.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L9"
                     /protein_id="HEG1168831.1"
                     /translation="MKVIFLQDVKGKGKKGDIKEVPAGYAQNYLIKNNLAKEATKSSI
                     SQLAGQKKAQEKHEAEILEEAKKLKEFLEKEDTVVELKAKAGEDSRLFGSIPSKQIAE
                     GLNKQYKIKLDKRKIELENPIRSLGYTNVPVKLHHEVTATIKVHVVQE"
     gene            31120..32487
                     /gene="dnaB"
                     /locus_tag="SCJ86_001258"
     CDS             31120..32487
                     /gene="dnaB"
                     /locus_tag="SCJ86_001258"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623169.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replicative DNA helicase"
                     /protein_id="HEG1168832.1"
                     /translation="MNEVWQDRIPPQNIEAEQAVLGSVFLDAETIIDAMEYIEPKDFY
                     RRGHQIIFETMIELNDRNEAIDVVTVKDRLEQANLLEDAGGLSYLSDLALAVPTAANI
                     VYYAKIVEEKSLLRTLIQTATGIVTKGFEQGEEVQTILDDAERSILEVSEKRNRSGFL
                     SIAEVLNSTIENIDRLAQNNEEITGLPTGYQGLDKMTAGFQPEELIILAARPAVGKTA
                     FALNIAQNVGTKTDRSVAIFSLEMGAESLVNRMLCAEGSIEASHLRTGQLSEEEWSNL
                     IVAMGSLSRANIFIDDTPGIKISEIRAKCRKLAQEKGNLGLILIDYLQLIEGNGKENR
                     QQEVSDISRQLKKLAKELKVPVIALSQLSRGVEQRQDKRPVLSDIRESGSIEQDADIV
                     AFLYRDDYYEREGGDDDDGNEPKNDNVIEVIIEKNRSGARGTVELLFIKEYNKFSSLS
                     PREEF"
     gene            33267..34559
                     /locus_tag="SCJ86_001259"
     CDS             33267..34559
                     /locus_tag="SCJ86_001259"
                     /EC_number="6.3.4.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002359213.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenylosuccinate synthase"
                     /protein_id="HEG1168833.1"
                     /translation="MSSVVVVGTQWGDEGKGKITDFLSENAEVIARYQGGDNAGHTIK
                     FNGVTYKLHLIPSGIFYKDKISVIGNGVVVNPKSLVKELAYLHEKGVETSNLRISDRA
                     HVILPYHIKLDQLQEDAKGENKIGTTIKGIGPAYMDKAARVGIRIADLLDKEIFEERL
                     RLNLDDKNRQFVKMFDSEPLDFDDIFEEYYEYGQQIKQYVTDTSVILNDALDAGKRVL
                     FEGAQGVMLDIDQGTYPFVTSSNPVAGGVTIGSGVGPSKINKVVGVCKAYTSRVGDGP
                     FPTELFDETGEQIREVGREYGTTTGRPRRVGWFDSVVMRHSKRVSGITNLSLNSIDVL
                     SGLETVKICTAYELDGELIYHYPASLKELNRCKPIYEELPGWSEDITGCKTLAELPEN
                     ARNYVRRISELVGVRISTFSVGPDRTQTNILESVWAQI"
BASE COUNT        10568 a         6748 c         6853 g        10530 t
ORIGIN      
        1 tcaagacgcc ctacacattt ggtttgtctt actttgttca gttttcaaag gtctacttca
       61 tcaggagtgt ttcccgattc gtttttggct gccgtagcaa cttttatatc atatcagaga
      121 gacaagtaat tgtcaactct tttttaaaag ttttttcgat ttcgtttttc gaaatcttcc
      181 agcaactcat ttatcataac ttatcgtttg ttatttgtca agaacttttt tgaagttttt
      241 ttcgatagaa tcaagcttcg caagtggttc ttttttaacg actcagttat cttatcatgt
      301 catttccaac atgtcaacaa cttatttttt attatttaat aagttattgg tgttcttttg
      361 aacaaataat aatatatcaa catcacatgt gaaagtcaat aagaaaatgc ttatttatca
      421 acatttctag cttacatgat cattggttag ttctggtcct ttttcgttcg tctttataac
      481 tccgagagac gtaaaatcct tttgttttca gtttaatgtt catgttcttg tcttattcac
      541 accaactaga tgtctcacct tctaaaattt tccttaacaa agtgctgctt ttatctattt
      601 ctgctttttt ttgctagaat aatagtaaca atatgactct ggaggattta tttatgaaaa
      661 ttttgattgc tgacgatgat aaagaaatcg ttgaattatt aagtatctat gttcacaatg
      721 aggggtatga agcagtaaag gcttatgatg ggaaagaagc attatctaaa atcagaacaa
      781 tccctgatat tgaattactt attttagata tcatgatgcc tgagatggat gggatgcaag
      841 tagtgaaaga gctacgaaaa gaatcccaaa taccaatcat tatgctgaca gcaaaaacta
      901 ctgatatgga taagatcaaa ggactggtcg ctggtgcaga tgattatgtg accaagccat
      961 ttaatccgtt agaagtaatg gctagagtaa agtctttatt acggcgtaca aaaatgcaga
     1021 ttgccccaga tcagcctgat gtattagaag taaatgcatt aatcatcaat aaagactctc
     1081 atgaagttaa aacaatcgat gggaaagaaa tccagttaac agctttggaa ttcggtatcc
     1141 tctacttgct tgctagtcat cctaatcgag tatttagcgc ggatgaaatt tttgagcgtg
     1201 tttggaaaca agaaagtatt gtctctgcta aaactgtcat ggttcacgtt agtcatttaa
     1261 gagataagat tgaagaagca actggtggtg aaaaagtcat tcaaaccgtt tggggagtgg
     1321 gctataaaat tgacgcaaga tagtaaacga gcaactgaaa aacgccgtcg gatcaatttg
     1381 acttcaaaag aaatcagcga attgttagcg gaaggaatca tcacgattat ccttttgctt
     1441 ctattaaatg tttctattct ggttgttatc agttcggtga tcaacagttc accttctttg
     1501 acgaatgcga tttgggattc aaagaatatt tttgcagaac gattgaacac tgatttgttc
     1561 tggaacggcc ggaattttat tattcccttt ttctttctat tagatattgg tgttttgtat
     1621 tggcgcttga ttcgaagata tcgccaaatg caattgcggc atatcatcag cgaacttcac
     1681 tacatcgcaa acggaaatta tgatcatcgt atcccttttg aactgagtgg tgacttaagc
     1741 cgagtggtaa caagtatcaa tggattggtg gatagtaccg tagcagcgat tgaagacgaa
     1801 cgtaaaatcg aaaaatcaaa agatgaattg atcacgaatg tcagtcatga tatccgtacc
     1861 ccactcacgt ccatcattgg atacctagga ctgatagaag atggccagta tcactcagaa
     1921 gaagatcttt taaagtatac gcatactgct tatatcaaag caaagcagat gaaatcttta
     1981 gtggacgact tatttgaata cactaaggta cgccagcctg ctgtcccggt gaatttttct
     2041 gcctttgaca tgatccagtt gatcgaacag ttagcagctg attttgagtt ggaagcttct
     2101 aaaaaaaata tccaaatact tgttcaatca aaagtagact cccttatcat ggatggcgat
     2161 accgaaaaac ttgtacgtgt cttcaataat cttttgacga atgcgttgaa atacggaaaa
     2221 ggagcaacga agatcgtaat cgaagtagaa cgtatcggtt cagaagttgt tgcaacagtc
     2281 aaaaataacg gagcaatgat cccacaacag gcaatcgata atctattcga ccgtttttat
     2341 cgtgtagaag aatctcgttc acaagcaact ggaggaactg gacttggttt agcaatcgct
     2401 caaagcattg tggctttgca cggcggatac atttatgcaa aatcagataa gcaatggact
     2461 tctttcatca tgcaccttcc aatcaagaaa aatgaaaaat tacctattga tacacaggaa
     2521 gttattgacg aaagctgaaa tattcgctat tcttattaca attattagaa taaatgaaaa
     2581 acacagaacc cagtacactt tattcttttt cagagagctg atggcaggtg taaatcagca
     2641 aaggataatt tggaagttcc agttcgttta cgtgcccttt cgaaataaga aaggtcggtt
     2701 caacagccgt tatgtgttca acaagtgcag ctgacgctga acttgggtgg taccgcgaat
     2761 ttcagactga ttcgtcccaa gaataatggg atggatcagt ctttttttat tttcaaaaag
     2821 gaggaaatca tatgttagat gtaaaaatga tgcgacaaaa tattgaagaa gtacaagaaa
     2881 aattgatgac tcggggcgta aaaaaagaaa cattagctga attcatggaa ctagatgaaa
     2941 atcgtcgtaa gctactagtc aaaagtgaag aattaaaaaa atatcgaaat gatgtttctg
     3001 ctgagatcgc ccaattgaag cgtgcaaaag aagatgcttc tgctaaaatc gctgaaatga
     3061 aagaagtcgg tggcaacatc aaagcattgg atactgaaat tgctgaagtg gatgaaaaat
     3121 tgaaacaaat tgcaactact ttgccaaacc ttccccatga ttctgtgcca gttggaacag
     3181 atgaagagga caatgtagaa atccgtcgtt ggagcgaacc acgtacattt gcttttgaac
     3241 caaaaccaca ctgggaaatt gcagaaaatc ttgatatttt agattttgaa cgtggcgcaa
     3301 aagtagccgg tagccgtttt gtctattaca aaggattagg tgctcgatta gaacgtgctg
     3361 tctacaactt catgcttgat cagcatattt acgaacacgg ctatactgaa atgatcactc
     3421 cttatatcgt caacagtaaa gcgatgtttg gtacgggaca atttccaaaa ttcaaagaag
     3481 atgttttcca attggagaat actgatctaa cattaatccc aacagctgaa gtaccattaa
     3541 ctaactatta taatgatgaa attttagatg gtgcagattt acctatttat ttcactgcac
     3601 taagccctgc ttttcgttct gaagccggaa gtgctggtcg ggatacacga ggactgattc
     3661 gtcttcacca attcaataaa gtagaaatgg tcaaattttc ggatgtagag cattcttacg
     3721 aagaattgga aaaaatgaca gctgatgcag aagatatctt gaaaaaattg catttgcctt
     3781 atcgtgtgat ggccttatca actggagaca tgggcttttc tgcagctaaa acttatgact
     3841 tagaagtatg gatacctgca caagatactt atcgtgaaat cagttcttgt tctaactgtg
     3901 aagatttcca agcacgccgc gcaatgatcc gttatcgcga taatgatgga aaaatccact
     3961 atactcatac tctgaatggt tcaggacttg cagttggccg tacagtagct gccattttgg
     4021 aaaactacca aaatgaagat ggttctgtca cagttccaga agcgctcgtt ccttatatgg
     4081 ggaatctgaa agttattaaa taaagaggct gtgaaaaagc cgcttagttt cgagtattaa
     4141 gaaaaatttt gtgaaaatgg ctttccacat ttttgcataa attggactta ataccgagaa
     4201 actggctttt gaacaccatt tatcgagagg ttgtgaaatc agcgaattaa cctgagtatt
     4261 agagaaaaaa atggaaaaat agtccctcat attttaccgt ttgttgaact aataccgagg
     4321 gtcagctgat tgaacaccgt ttagtggaga ggtcgtaagc ctacccccca gctcctagtt
     4381 acaaggacaa attcaaaaaa cagctcttca tattttatga atgttgtact tgtgattcgg
     4441 aggtggcaag ggaacactgt ttattcaaaa agaggctggg acagaagcgt ttaactccaa
     4501 gaaataagaa gaaattcacg aaaattgctt ttcaaatttt tgtgaatttc ttcttatttc
     4561 cgaaggagtt gcttctactt ccgccgttta tacgtgttta gagcgtgaga caaaagtgtt
     4621 ctttactttt gtcccacgct ctttaatgtt tttaagattc aactgaatag tttggtgctt
     4681 cttttgtgat ttgtacatca tgagggtgtg attcttttaa tccactgccg ctcatttgga
     4741 taaattgcgc ttcatcacgt aattgctgta gattggctgc cccaacataa cccattcctg
     4801 aacgcaaacc gccgatcatt tggaaaataa tatctgcaac acttccttta taagcaaccc
     4861 gaccttcgat tccttctggt actaatttat ttgcttcatt gacactacct tggaagtaac
     4921 ggtcttttga acctttttcc atcgcgccta atgagcccat acctcggtat gttttgaatc
     4981 gacgtccttg gaagatttcg aattcacctg gtgattcatc tgtccctgcc aacatacttc
     5041 ctagcatcac tgcatgtccg ccggcagcta atgctttgac gatgtcacca gaatatttga
     5101 tccctccgtc tgcaataatc gctttgccat attgacgagc aactgatgct gcatcataaa
     5161 tagctgtgag ctgaggtaca cctacaccgg ctaccacacg tgtcgtacaa atcgatcctg
     5221 gtccgatacc aacttttacg acatctacac ctgcatcata taaagctttt gtcgcttcag
     5281 ccgtcgcaac gtttcctgca atcaatgttg catctgcaaa agttgaacgg atttcttgaa
     5341 tttttcggat cacacctgca ctatgtccat gagctgtatc gatgatgatt gcatctgccc
     5401 ctgcttctaa taaagcattc gcacgctcaa atgtatcgct tgttacacca acggctgctg
     5461 ctacaagcaa tcgaccatgt tcatcttttg ctgcatttgg aaattcgatc actttctcga
     5521 tgtctttgat cgtgatcaat ccgcttaaac gaccttcgtt atctacgatt ggtaattttt
     5581 cgattttatg tttttgcaaa atcttttctg catcttttag agaggtacct actggggcag
     5641 tcactaagtt gtcttttgtc atcacatcag caatcggcat gtgataatct gttacaaaac
     5701 gcatgtcgcg gtttgtaatt atccctacca attttctgtt ttcaagtgtt tcaacgattg
     5761 gcacaccact gatacgatat ttgctcatca attcttctgc gtctgctaca agatgctgtg
     5821 gcgttaagaa gaacggatca atgattacgc cactttcaga acgtttgact tttcttactt
     5881 catccgcttg ctggctgatc gtcatgtttt tatggatcac acctagtccg ccttgtcttg
     5941 ccatagcaat ggccatcttg ctgtcggtaa cagtatccat acttgcactg atgattggaa
     6001 tattcaactt aatattttta gctaattgta cgctcatatc cacttcgttt ggtaacacgt
     6061 gactttctgc tggtatcaat aagacatcat caaaagtaaa tccttttttc gtgaactttg
     6121 tttcccagtt ggacattttt agtaccactc ctctaattta gtttatctat taaaataatt
     6181 gattttttca tttaagtcaa tgatctactt ggaatcaagt tagctttctc atagaatacc
     6241 aatataaagc gtttacatct gtgtatatta aaagagattg taaaaaagct gtcctgcatc
     6301 aagtaataag aacagccaat caaaaacagc ttctcatgtt tttgaattgg ttgggtttat
     6361 taccgcagat gcaaactttt gaacaccgtt tagtggagag gctgtgaaaa agctgcttag
     6421 tttcgagtag taagaaaaat tttgtgaaaa tggctttcca catttttgca taaattggac
     6481 ttaataccga gaaactggct tttgaacacc gtttatcgta aaaagggact gtgacaagac
     6541 ttttgtcaca gtcccttact attatttaac tatggataaa caaaatgagg ttctttacta
     6601 cccctagata tctagcaaaa tagctgatct ttttgctagt ttatcttctt gggacggata
     6661 aacttccaat aatcccgtat tttttcttca agtagtaacg gattgctaaa gcagttgccc
     6721 cgataatgat aactactact ggatctaata ctggattgat aactggtggc aatagtgttg
     6781 tcgcagaaaa cagtacgacc caaagaagtg tcgttccacc taggataagc aatgtcttac
     6841 ccattcctgg tttcttgctt cgatctgctc cgggatattc atattgatag atgtatttgt
     6901 acatcatata aaagacccag ccaccgattg cagaacctag aacgagtgta gaaaagccat
     6961 aagctggcgt tgttccacgt gaaaacaatc cggtaatcgc aatcatgata cttaatagac
     7021 caagcaacag caacgtatta tctaaccaca tcaataaagg ggtggatgaa ttttttactt
     7081 ctgctggctt attgatgatt gcttccgttc tttcagagac tgtcccaaac aattgacggg
     7141 ctgttcttcc gcctttttgt tctgcaacta aagtaggcag aatgtcatga agggctttta
     7201 ctttttcttc ttcagacaaa ttcgctgctt ctaaagattt tttcaaatca aaaatgtact
     7261 gttgatttct tttggttaat tttgtttcta aactattgtt ttcttttacg attgctcgta
     7321 attgttctgg ttccattatc taatagtcct ccttagccgt tccttttctc aacggtcgtc
     7381 acaaaagaca ttctaccata ttctacagct agtttgatag cccttttaca cattaaagcg
     7441 gaataacatt acgtcgccgt cttgtacgac ataatctttt ccttctaatc gaacacgtcc
     7501 agcttctttt gctgcatgca tgtttccgta atgatccaag tcttcaaaag agacagtttc
     7561 tgcacggata aatccgcgtt cgaaatctga atggatgatt cctgctgctt gtggtgcttt
     7621 gattcctttt ttgaatgtcc aagcacgtac ttcttgttcc ccagcagtaa agtaagtagc
     7681 taagcctaac aaatcataag cagcacggat caactgatcc aaacctgatt cttcgattcc
     7741 taaagcttct aaaaattccg ctttgtcttc ttcatctaat tctgcgattt cttcttctgc
     7801 acgagcactg accacgatca cttctgcatt ttcttctgca gcaaagttgc gcacttcttg
     7861 tacataatga ttcgcgtctg catctgctac gtcatcttct gagacattgg ctacataaag
     7921 aattggtttt gtcgtcaata aaaatagtga tttaacgatt ttttgttctt cttcagtaaa
     7981 ttcgatcgtt cgggcagatt ttccttcttc cagtacaggt ttgattttat ccagtacagc
     8041 tagttctgcc actgcatctt tatctttggt tttagcaact tttgctacac gagtatagcg
     8101 tttgttgata gattctaagt ctgctaacac tagttccaag ttgatcgtat caatatcttc
     8161 tagtggatca acacgtcctt ctacgtgagt aatattatca tcatcaaaac agcgaacaac
     8221 gtgacaaata gcgtctactt ggcggatatg gcttaagaat tgatttccca aaccttctcc
     8281 tttacttgcg ccttttacga ttccagcaat atctgtaaat tcaaaagtcg tcggaacagt
     8341 ttttttcgga tgtacaagtt ctgttaagcg ttgcaaacgc cagtcaggta cttctaccat
     8401 ccctacgttg gggtcgatcg tcgcaaaggg atagtttgct gcttctgctc ctgcttttgt
     8461 gatcgcatta aacaaagttg attttcctac gttgggcaac ccaacaattc cagcagtcaa
     8521 tgccataaat ttattcactc tttctctgca tttttttcaa ttactttttt taatttcttt
     8581 tcaaaatctc gacgggtcat catgacgata tgtccgcaat tcatacatct gattttgata
     8641 tctgcgccca ttcgaatgac ttcccagcga ttcgtctggc aagcgtgagg ttttttcatt
     8701 tcaacgatat cgtgcaagtc gtacataaaa atagcctcct ttttgtttat tcttcatcaa
     8761 attgaatatt caagatatca agaattcgag tcaaatcttt ttgagaaaga tattcaatct
     8821 cgatcttacc tttcccattt ttttcttgga tagctacact tgtaccaaat ttatccatca
     8881 aacgatcttc gctttctcgg atataatatg gtttttcctt tgctagcctt gggatttttt
     8941 tcttttcttt tccatcattt tgatttaact cagaaacaag ctgttccagt tggcggacag
     9001 ttaaattttc agttactgca cgattggcta atttcaagat caatgtttta ttttttaaac
     9061 ctaacagcgt acgggcttgt cccatagata aacgctgatc ttgtaccatt tccttgacaa
     9121 gatccggtaa agaaagcaag cgcaaataat tggcgatata aggtctgctc tttcctaaac
     9181 gactagctac ttctgcttgt gttagtttca ggtttttcat cagcatctca tatgcttctg
     9241 cttcttctaa cggattcaga tcttctcgct gcaagttttc cagcactgct acttgcatca
     9301 tcgcttcttc gtcaaagtct ctaacgatag cagggatcgt ttctttttca gctagtttgg
     9361 acgctcgaaa acgtcgctcc cctgcaataa tctcatatcc ttttactgcg gaacggcgta
     9421 cgatgatggg ttgaaagaca cctgattgtt caatagaatt agctagttcc tgcaaagata
     9481 cttcttcaaa tgttttacgg ggctgatacg gatttggtcg cagctcattc aatggtagct
     9541 ggacaacggt atcatctttt acatccaccg tctctaagtt ttctaaatct tgaaacagtg
     9601 catcgattcc tcggcctaat cctttacttt tcttcacgac ttaccacttc ctttgccaat
     9661 gcttggtaga cttcagctcc tcgagaacgt ggatcgtagt cgatgatcgg ttttccatga
     9721 ctaggtgctt ctgataaacg aatatttctt ggaatgatcg tttcgtaaac tttttcgcgg
     9781 aagtatttac gtacttcttc tacgacttca tttcccagat ttgttcgtgc atcatacatt
     9841 gtcagtaaga ctccctcgat ctctaattct ggattaaagt gtttttggac taaacgaacc
     9901 gtattcagta attggcttaa tccttccaat gcgtaatact cacactgaac aggtatcaaa
     9961 atcgaatcac ttgctgtaaa tgaattgatt gtcagatggc caagagaagg cgggcaatcg
    10021 atcaaaatat aatcatactg actgcttact tctgctaaag atccttttaa tcttgattct
    10081 cttgccatca ttgacgtcaa ttcgatctct gctcctgcta attggagagt cgcaggcaca
    10141 atactcaaat tttcgtgttc agtgatcaaa gttgcctcat caattggcaa ttcattgact
    10201 agaacatcat agatgtctct tgtcacatca ggttttctga tcccgacacc actggtagca
    10261 tttccttgtg catccatatc cactagcaat acttttttgc caaggctagc aagacaagca
    10321 cctagattca ccgtcgtcgt tgttttgccg acgccacctt tttggttcgc aacagatatt
    10381 atttgtgcca tctcttctcc tcctatttga ttggctgctt attaggcagt ccgggctttc
    10441 ttgggtattt ctttggggtt ggtttctttt tttgaataac gatgatatgc cgttgatcag
    10501 agacattcgg caaggtaacc gcctcttctt tgaggaattt accacctaat aaagcaatcg
    10561 cttgttttgc ttctgtcaat tcctcttcac tttttgctgc tttcaatgca taaaaatagc
    10621 cttcttgttt agccaagggc aaacacaatt ccgctaacac attcaaacga gcaaccgcac
    10681 gagcagtgac aaggtcaaat gatgcacgaa agtgaggatt ttgcccaaat gtttctgccc
    10741 ggtcatgata taaggaagca tcgattccta ataatcgggt taactcagat aagaaggtta
    10801 ttcgtttatt cagagaatca acgatcgtta tctgcagttc tggaaaggca atttttaaag
    10861 gtatgctagg aaaccctgcc cccgaaccaa catcgcataa agataaaggt tgcgtaaagt
    10921 ctacttcaaa tgctaatgta agtgaatcgt aaaaatgctt cagatatacg tctttttcat
    10981 ccgtaatagc tgtaaggttc atcttttcgt tccattcgac taatgtccga taataaatag
    11041 cgaattgatt catttgatga tcggtaagct ctatcccttt ttctgccaag gattggcgaa
    11101 attcttctgg tatcataaat attcctttca ttgtgaaaca gcaattgttt cacagattct
    11161 ctccctactt taacgcaaaa tctctagaca tgcaatcctt ccatatggat tcttaaaaat
    11221 ttgaaaagta agaaaagccg atagactttt tctttctagt atgactcata aaatatacac
    11281 agaagagaga actagctttg agcaatttct gcaaaaacta gttctctctt ctatttatta
    11341 ttacgtatta cgctggttca ctgactttcg caatcttccc ttgttcgata tagaccatta
    11401 ggatacttag gtctgcaggg tttacgccac tgattcggct agcttgggca attgtttctg
    11461 gttggatttt ctgcaatttt tgttttgctt ctgttgctaa tccgttgatt gctgcgtagt
    11521 cgatgttttc tggaatcttt ttcgcttcca tgcgttttaa tttttctact ttttccatgg
    11581 cttttttgat gtagccttca tatttgattt ggatttctac ttgttcgatt tcttttcgtg
    11641 tcaattcttc attttctgga ataaaccgta gaagttctgc ataagtaatc tcaggacgtc
    11701 ttaaaaagtc gcttgctagt acaccatctt ttaatggtgc cgcattaatc gttgctaaaa
    11761 agtcttggac ttcttctgtt ggtttgattc gtacattcat caaacgtttg atttcttgtt
    11821 caactgcttc ttttttcttc aaataattct tgtattgatc ttcttttact aagcctacac
    11881 ggtgacccat ttctgttaat cgcaaatctg cattatcatg acgtaaaagc agacggtatt
    11941 cagcacgtga agtcagcaaa cgataaggtt cgttcgttcc ttttgttacc aaatcatcga
    12001 tcatgacacc gatgtaaccg tcacttcgtt tgatcactaa aggttctttt ccctgaactt
    12061 tcaaagctgc attgataccg gcaatcagcc cttgtccagc cgcttcttca taaccgcttg
    12121 taccatttgt ttgtccagct gtataaagat tttcaacgac tttagtttct aatgtcggac
    12181 ggagctggtg tggctctact acgtcatatt cgatagcata tcctgtacgc atcatttcta
    12241 ctttttctaa tccagcaata gaatgaagca tgttgacttg aacatcctct ggtaaagaag
    12301 tagataaacc ttgaacatag acttcttccg tatttaagcc ttctggttcc aaaaataatt
    12361 gatgacgtgg tttgtctgca aaacgcacga tcttatcttc gatggatgga caatagcgag
    12421 cgcctacccc ttcaacgatc cctgtaaaca ttggggcacg atgtaagttg tctcgaataa
    12481 tttggtgagt cgtctcacct gtataggtca gccagcaaga ttcctgatct agtttatagg
    12541 cactatctgg tgtgctgaag ctaaaatgat tcggctcttt gtcaccaggc tgctcttctg
    12601 taacagaata gtcgatcgta cttgatttta ctcgaggcgg tgttcctgtt ttgaaccgtt
    12661 cgatagcaaa gccaagttct tttagatgat tcgctagtcc aatagaaggt tgagagttat
    12721 tcggacctga tgaatatttc aattcaccga taatgatttc tccgcgaagt gctgttccgg
    12781 ctgtaataac aactgctttg gcccggtagc tcgctcctgt ggacgtcact actccatgac
    12841 aaacaccatc ttcgacgatc agttcttcga cgattccttg tcttagcgtc aaatttggtt
    12901 ctttttcgat cgtatgtttc atttctgttg aataagcatg tttgtctgct tgtgctcgca
    12961 atgctcggac agctgggcct tttcctgtgt tcaacatacg catttggata taggttttat
    13021 cgatattctt acccatttct ccgcctaacg catcgatttc tcgaacgaca acgcctttag
    13081 ctggtccgcc tacagaagga ttacatggca tgaatgctac catgtccaaa tttattgtca
    13141 gtaataatgt ttttgatccc atacgtgcag cagcaagtgc agcttctgaa ccggcgtgtc
    13201 ctgcgccaac aacgatcacg tcatacgcat ctgcttcata atgttccaat cgtttgttcc
    13261 tccttttatt ttcctaaaca aaattgactg aataattgag tgattagctc atcttgtaca
    13321 ctgtcgccga caacttctcc tagatagtcc cagcaacgcg tcatatctat ttgaacgaga
    13381 tccacaggca ttcctgaatc aatacctgca atgacttctt caagtgagag ggaagctttt
    13441 tctaacaacg cgatatggcg ggtattagaa acataagtag cgtctctttc tcctgtttct
    13501 ccgctgaaga ataaatcaga aatagctgat tccaaacgat ctagtccgtc gtttttagct
    13561 acggaaattg aaaaaactgg ttcattttcg atcaatttct tcaatttttc ttgttccagc
    13621 tgtgctggca aatctgtttt atttagaaga atgatccgtt tcaatccact tgtcgcttcc
    13681 aataattgtt catcttctgc tgtcagtggt tcgctttggt ttagaacaag taagatcaag
    13741 tctgcttctg ctaatgcttt tcggcttctt tcgaccccga tccgttccac cacatcttct
    13801 gtttcccgaa ttccagctgt atcgattagc ttgagcggta ccccccgaac attgacatac
    13861 tcttcgatta catctcgagt cgttcctgca atatccgtaa cgattgcttt ttcttctctt
    13921 aacaaatggt ttaacaaact tgatttgccg acatttggcc ggccgatgat tgccgtactt
    13981 aacccttccc gcagaacctt cccttgcttg gatgtagcaa gtagcgcttg gattctttgt
    14041 tggatcatct gcgctttttc aagcaataac ttcgtagtca attcttcgac atcatcatat
    14101 tctggataat caatatttac ttctacttgt gctaatgtct ctaaaatctc ttgtctcaat
    14161 gagcggatca aagcagacag attgccgtct aactgattaa gagctagtcc cattgctttg
    14221 tctgtttttg cccgaatcaa atccatcaca gcttctgcct gtgaaaggtc aacacgacca
    14281 ttcaagaatg ctcgttttgt gaattcgcct ggctcagcta atcttgcacc ttctcttaac
    14341 aatagctgca aaatttgatt gactacaacg atcccaccgt gacaattgat ttctactaca
    14401 tcttcccgcg taaatgtctt tggtgctctc atcacggaga ccatgacttc atcgacaagc
    14461 tgattgctct tgggatcaac gatatgtcca taatggatcg tatgacttgg tacttctgat
    14521 aatcgtttgt ttccagattg ataaacttta tctgctagtt gcacagcttg atcgccactt
    14581 aaacgaacga tactgattgc accttctcca ggtggggtag aaattgccgc aatcgtatca
    14641 aattctaaag taatttcagc cattttttca cttccttttt cattttgcgc acaaaaaaag
    14701 tgcccactcc accctttatg cacaataaac ggtgaacttc gcactttgac tgcttgttct
    14761 tgattaaatt ctggtcatag attatcacac ttgctataag gaatcaagtg tttaaaacaa
    14821 ttagatgaag ttgagcctgt tctgcttcca ttaccttata tttaattgtc cttggatgtc
    14881 tctttgcgtt ctttggttta ttcgaagaga agatactgta taaacaaggc tttatctttc
    14941 ttaaaaaaaa cggtacacta aatggtagcg ctatctaaac tatacacttt tgaaaggaga
    15001 aaatatgtga gcccgttcag atctgctgca ttggaagaag aaagtgaccc tcggatcaac
    15061 gatgtcgttt tatctacagg gaatatcaac cagaaatata tcgtccgcga tatcgttttt
    15121 gttgtggaat gcttagaagc tgatttgttt gacccatctt ttcccaaaga tgaccttttc
    15181 ttaacaacga agaggaaatt aaagacgaag gtacttgaat atggtgcgaa tgcagtaatc
    15241 aactgtcatt ttgagtatca gcaaaaaaaa cgcgacggaa agtattttct ggaacttttt
    15301 gcttatggaa cagtgatcca atttatccaa acaacgattg gttaaatgaa acagacaaaa
    15361 aaagcggatc aaaactgaag acagtttcga tccgcttttt tctatcaaag ttagtgacta
    15421 cttcatcgta tactctgata agaatgattt ctagtaaaat atacttggtt tgaaaaactt
    15481 tgtcaacgtg atttattcaa aaagtaatgg aatcaataat attttttagc aggttctact
    15541 actaaataac gataaggttc ctcaccttcc gaatgtgttt ttacatgttc atctttgctt
    15601 aatgcagcat ggatttgttt acgttcaaat gcaggcattg gttctaagaa aacaggctgc
    15661 cctgtccgtt ttactttttc agccgtgcga tcagctaaac gctgaatgat ctcttggcgc
    15721 ttttcacggt aattgcctac attgactact acagacagct tatttcttgc taaacgatgg
    15781 atgaagactt gtgccagata ttgcaatgca ttcagtgttt ttccatgttt cccaatcagt
    15841 atgccttgct tttgtgtttc taaatggaaa acaaccatat tatttgattc tctttccatt
    15901 ttgacaagag ctggagcatc aagctgtttt gaaatttcag taagataaac tgccaattcc
    15961 gttaaagctg cttcatcacc tagctcactt acttcttctg ttttttcttc aagaatcgtt
    16021 tcgttgacta cctcgatctc agctgttggt tcttcttcca aaatttcttc tacagtctgt
    16081 tctactgttt cagaaacttt ttcgctgata cttggctgga tcgaaagccg tgcattcttt
    16141 cgacccattc ctaggaaacc tttttttcct tcttccagta tttcgatttc gacttggtct
    16201 ttggccaaac ccaaatcagc cagacctagt tggacagctt cgtctattgt tgcagcttca
    16261 taaatcggca ttagcattac ctcccctatt atttccgttt tttcttagga ttcattgctt
    16321 tcttaagagc acgttctctt tcgcggattc tccgtgcttc ttcttcacgc tcttttcgaa
    16381 ttttgaaagg gttgttgatc agcagcgttt gtacaacttg gaaggcattg gaaactaccc
    16441 agtataatga aagaccgctg gctaaattga cacccatcac taaaatcatt acaggcatca
    16501 cgaaattcat gatcttcatt gatgcattcg attcaatttg actcatgcta gataaatacg
    16561 tacttgcaaa agtaaagatt gctgctaaaa tcggcaaaat aaatgttgga tccgcgttcc
    16621 ctaagttcaa ccataagaaa tgcccttgtt tcaactctgg tacacgtgaa atagattgcc
    16681 ataacgccat caagattggc atttgtacta ataaaggaag acaaccagca tatggattga
    16741 cgccattttc tgcatataaa cgctgctgct cttctcttaa cttactttgt gtttccggat
    16801 ctcttgaact atattgttgc tgcagtgctt ttaatttcgg ttgtaattct tgcgttttac
    16861 gcatgctctt tgtttggaaa tgcatcaacg gcaataggat cacgcggatg atcagtgtaa
    16921 ataaaatgat ccctatccct gcattaccaa atgacaacgc cttgatcgcc tcagcaaagt
    16981 aataaacaat ataacgatcc caaattcctg tactctgtgc gctgacttcc ccagttccgc
    17041 aaccggataa gacgatgacc aaactaacga taccggccat caaaagtaaa cgtttgtatt
    17101 tcttcactta ctcttttcct ctctgtcgat tatttttgca agttttaaga cgtggactaa
    17161 attggagcga acctcatcat aagtcaaccc cttcacactt ggtctagcaa tcacaataaa
    17221 atccaactcc ggatcaattt cttcttttat agaataaact gctgctcgga tttttcgttt
    17281 gacttcattg cggtttaccg cgttgccgac cttttttcct acagataaac caactcgaaa
    17341 atgaggctgt cccggttttt ccaaacggta aacgacgaat ttgcggttag caaaagaatc
    17401 tcccgtatgg aacacggctt gaaactctcg ttcttttttt actcgatacg cttttctcat
    17461 tagttttctt tccttttcta tctctcactt acgctcataa tcataccata attacgattg
    17521 tcctttcatc atttccctaa aaaagagtat aaaaaaaacc actgaaaccc cagtggccta
    17581 agctgaaaga acttttcttc ctttgcgacg acggctagct aacacacgac gtccattttt
    17641 agtactcata cgtttacgga atccgtgaac tttttgacgt ttacgtttat ttggttggta
    17701 tgttcttttc atatattcca cctccaaaat gttcttatct attttataaa cacagacata
    17761 ctttgatagt ataacgacaa agtagtgatt ttgtcaatat aaaatagaag atcgattgtt
    17821 aaaacttatc cacaagatta tttttttcta tgggaatcct atgtggaaaa agttatgcac
    17881 agggattatc cacagataat aaaaggctgg ttttacacag aaaacacacg tgtaaaaaaa
    17941 gtaatccaca atcaggcgat ttcctgtgta ttccgtctcc taactattgg tatatctgta
    18001 ttttttatcc acagacgcaa gaggaaatct attggttttt acgagttttc cacctgtgca
    18061 ttagtggtgt ggaaaacttt ttttgacaac tgtgtttttc tcttttttct ttttgtcgat
    18121 tgtgtggata attgtaatta aaaaattttt ttcttcggtt tctgtggaaa actattgaaa
    18181 aaaatgctaa attagtacta gttattcact ttttaatgaa ggaggaggcc ttatatggta
    18241 tccctcgatg ctttatggaa tgaattaaaa gcaacatacc aaaaggattt atcgcctgct
    18301 agctataata catggatcga aacagcgaac cctcgaaccc ttgatcaaaa ccagctcgtt
    18361 gttgaggttc ctagcaagat tcataaagaa tattgggaaa aaaacctggc gacaaaaatc
    18421 gttgaagtcg gttatatgtt atcaggaaat gagattatcc ctcgctttat tactggcgaa
    18481 gaagcagagc aagaagaagt tatagaagaa aaaaatccaa aagttgtggc acctagtccg
    18541 ctaaaaaaag ccatgctgaa ccctaaatat acctttgata cgtttgtcat tggtaaagga
    18601 aaccagatgg ctcatgcagc agcgctcgtt gtagcagaag atccaggttc tatttataac
    18661 ccgctcttct tttatggagg cgtgggactt ggtaagactc acttgatgca tgcaattggt
    18721 catcaaatgc tgcaaagtca gcctaatgcc aaagtaaaat atgtaagcag tgaaactttt
    18781 gctaatgatt tcatcaattc gatccaaaac aaaacagcag aagagttccg tcaagaatat
    18841 cgaaatgttg atttactgct agtggatgac attcaatttt ttgcagaaaa agaagctacg
    18901 caagaagagt ttttccatac tttcaatgcc ttgtacaatg aagggaaaca aatcgtacta
    18961 accagtgatc ggttgccaaa cgagattccg aaactgcaag aacgccttgt ttctcgcttt
    19021 gcatgggggc tgtctgttga tattacaccg ccggatcttg aaacaagaac agcgatttta
    19081 cgtaaaaaag cggcggctga acgtctagag atcccagatg acacactaag ctacatcgca
    19141 ggacaaattg attctaacat tcgtgaatta gaaggtgcgc ttgttcgagt gcaagctttt
    19201 gctgcgatga atagtgaaga tatttcgact agtctagctg cagacgcact gaaaacgtta
    19261 aaatcaggaa aaggacatcc tcaattgtca atcctgcaga tccaagaaga agtggcaaag
    19321 tattatcaca tccaattgaa agatctcaaa ggaaagaaac gcgtcaaatc tattgttgtt
    19381 cctagacaaa tcgctatgta tttggcacgt gagctcacag ataattcttt accgaaaatc
    19441 ggagctgaat tcggtggcaa ggatcataca acggttatcc atgctcatga aaaaatccag
    19501 cagctgatgg attctagtgt cagtatgcaa aatgaagtat cagaaatcaa aaacttttta
    19561 ctaaattaat ttgtcgattc tatcaactga aaatagcatg tggataaaag aaaaaaacgt
    19621 tcaaaagtta tacacacttt ttccacagct gttttttcgt ttctctctta cgttttttga
    19681 gttttccaca gaatcaacat gccctattac ttttattatt ctttttaact aataataaat
    19741 aaacaaacat tcaaaggaga tacatcatga aagttacttt aaaccgagct agctttatgc
    19801 aggaattgca aactgttcaa cgagctattt caagcaaaac cacgatccct attttgacag
    19861 gtgtaaaaat cacactgaca caagaaggtt tgactttgac ggggagcaac gctgatatat
    19921 caattgaaac ttttttgtct gttgaaaacg aaaaagcaaa tatgcaaatc gaatctactg
    19981 gttccattgt tttacaagca cgtttcttta gcgaaatcat tcggagactt cctgaagaaa
    20041 catttacttt agaagtttta gaaaataaac aagtagcgat cacttctgga aaagcgaatt
    20101 ttatcgtaaa tggattagat gcagataact atcctcatct tcctgttgtc gaaagccata
    20161 accagatgaa attacctgta cacgtattga ctaaactaat caacgaaaca gtttttgctg
    20221 tctcacaaca tgagagtcgt ccaatcttga caggtgtgca tttcatttta tctgataatt
    20281 ctttattagc tgtagctact gattctcacc gtctaagtca acgcgtgatt ccagtagaac
    20341 aagcggctga tcattttgat attgttattc ctggaaaaag tttgatcgaa ttatctcgct
    20401 cattaacaaa tgaagaagaa atcgtcgaaa tcagcattat ggaaaaccaa gtgttgttca
    20461 aaacagaaac gatgtatttc tattcccgtt tgctagaagg aaactatcca gataccaatc
    20521 gtttgattcc ttcaagtttt aatacagagg ttgaattttc tgttcctagc tttttagcgg
    20581 cgatcgaacg ggcttcttta ctttctcatg aaggccgtaa caacatcgtt cgtttatcta
    20641 ttcgtccaga tgcggttgtt ttatacggaa attcgccaga aatcgggaaa gtagaagaaa
    20701 gcttgagtta tacagcaagc agtggcgatc cattagatat ttctttcaat ccggattata
    20761 tgaaagcagc gttgcgtgca tttggagata tgagcatcaa agtgaaattt atctctgcta
    20821 ttcgtccatt tacattggaa ccaacggaag atggcgttca atttattcag ctgattactc
    20881 ctgtacgtac aaattaacaa tgattttcaa tgttcttgaa aatttaattg aaaagaaata
    20941 gaaaaaataa aattttaaac agaaggccct aaaatctctt ttaaggcttt ctgtttattt
    21001 ttgtttaaat cacctttttt agattaaatc gtcttatatg gaaaaatata tggataaatt
    21061 tgttttattg cgaaaaaaaa ggtataatag agtatacttt taataatgaa aatgagggat
    21121 gggcattgaa acaacaagtc attctaaata cggaattcat gacgttgggc caaatgttga
    21181 aagaagtcac agttatcggt agtggcggac aagcaaaatg gtatttggca gaaaacactg
    21241 tgttagtcga tggcgaacca gaaaatcgtc gtggacgcaa actttatccc ggcatgatgg
    21301 ttgaagttcc agaagtagga acttttttta tggtaaaaaa aggaaacgac gataatgagg
    21361 ctgaataagc tgtatttaaa aaattatcga aattacgaag agctgaacat cgaattttcc
    21421 aagaacctcg tcatcttttt aggagaaaat gcgcaaggaa aaacgaatct tttggaaagt
    21481 atctacgtat tagctatgac gcgaagtcat cggacaagta gtgagcagga attgattggc
    21541 tggactgacg atcaggcgat gattcaagga gagatcacaa aaggttcatc cactcttcca
    21601 ttagaaatct tattatctaa aaaaggccga aaaaccaaga tcaaccatat cgaacagaaa
    21661 aagttaagta cttacgtggg acaaatgaat gtcattttat ttgcaccaga agatctttct
    21721 ttagtaaaag gcagcccgca gaccaggcgg aagtttttag atatggaatt aggccagatc
    21781 aatccaatct atctttatga tcttgtccaa taccaagcaa ttttgaaaca aagaaaccag
    21841 tacttgaagc aattgaatga aaaaaaacaa actgatttgc tttatttgga tattttgtcc
    21901 gaacaactag cagcgtttgg cagcaaagta ttgaaagcaa gagcacagtt cgtcaagcga
    21961 ttggagtttt gggctaattc tttgcatcag caaatcaccc atcaaaaaga gcagctggaa
    22021 atcgagtatc tcaccgcagt cgattcttta gaaacacata cgcaagagca aatccaagaa
    22081 caatttctcg cacttttgaa ccaaaataaa aagaaagacc tttttcgggg aacaacgaca
    22141 gttggtcctc atcgtgacga tctttccttt tttattaatc agaaaaatgt gcagacttat
    22201 ggatcgcaag gacagcaaag aacaactgca ctaagtgtca aattagcaga aatcgatttg
    22261 attaaagaag agacagggga atatccaatc cttttgttag atgatgtgat gagcgagctg
    22321 gatgataatc gtcaactcca tctgctagaa acaatcgaag gaaaagttca aacgttctta
    22381 acaacaacga cacttgacca tgtaaaaaat aaaatgactg ttgaacctga gattttttat
    22441 gttcaacaag ggaaactaaa aggagaagaa actgaatgac agaagaaaga agtttagtag
    22501 aacgcgctaa agagtatgat gccagtcaga ttcaagtact tgaaggactt gaagccgttc
    22561 gtaaacgacc tggcatgtat atcggttcga ctagttctga gggtctgcat caccttgttt
    22621 gggaaattgt cgataattcg atcgatgaag tattagcagg ttttgcaacg aaaatccatg
    22681 tgattattga aaaagacaac agtatcaccg ttattgacga cggtcggggg atacctgtag
    22741 atatccaggc aaaaactgga cgtcctgcag ttgaaacggt ctttactgtt ttacatgctg
    22801 gaggaaaatt cggcggtggt gggtacaaag tttctggtgg acttcacgga gtaggatctt
    22861 ctgttgttaa cgcgctttct acacagcttg atgtcaaagt atacaaagaa ggaaatgtgt
    22921 actatcaaga atatcgtcgt ggagctgtag tggacgactt aaagattatc gaacaaacgg
    22981 atcgccatgg aacaacggtt cactttacgc cagaccctga aatttttaca gagacaactg
    23041 agtttgattt ttcaaaattg gctacccgga tccgtgaact tgctttcttg aaccgcggga
    23101 tgcgtatatc gattgaagac aaacgagaag aagaaccagt catcaatgaa taccattatg
    23161 acggcgggat taaaagttat gtcgaatact tgaatgcaaa caaaaccgtg atttttccag
    23221 aacctgtata tttggaaggt gaacagcaag atatcgcagt tgaagtctct atgcaatata
    23281 cagacggcta ccattccaat atcatgagtt ttgccaacaa tatccatacg tatgaaggcg
    23341 gaacacatga atcaggattc aagactgctt taactcgtgt gataaatgat tatgcccgaa
    23401 aacaaaagct gatgaaagaa aatgacgaca acttgaccgg tgaagacgtg cgagaaggat
    23461 tgacagctgt tatttctatc aagcatcctg accctcaatt cgaaggacaa acaaaaacaa
    23521 aattgggcaa ttccgaagta cggacagtca ctgatcggtt atttagcgaa catttcatga
    23581 aattcttgct ggaaaaccca agtgtgggcc gtcaaattgt tgaaaaagga ttactagctt
    23641 ccagagctcg tctagcagcg aaacgtgcgc gtgaagtaac gagacgtaaa ggtgctttag
    23701 agattagtaa tctgccagga aaattagcgg actgctctag caacgatcct gaaaaatgcg
    23761 aattgttcat cgtcgaagga gattcggccg gcggatcagc taagcaaggt cgtaatcgag
    23821 agtttcaagc catcttgcct attcgtggga aaatcttgaa tgttgaaaaa gcaagcatgg
    23881 acaagatctt ggcaaatgaa gagatccggt ctttatttac tgctatgggt acagggtttg
    23941 gagaagattt tgatgtatcg aaagctcgct accacaaatt agttatcatg actgatgccg
    24001 atgtcgacgg cgcgcatatc cgtacattgc tgctgactct tttttatcgt tacatgcgtc
    24061 ctattgttga agctggatac gtctatattg ctcagccacc tttatacggg gtaaaacaag
    24121 ggaagaatat tacgtatgta caaccaggaa aaaatgcaga agaagagttg aaacaagtcg
    24181 ttgcttcact tcctgcctca ccaaaaccaa gcgttcagcg ttataaagga cttggagaaa
    24241 tggatgatca tcaactatgg gaaacaacta tggacccaag caatcggatg atggcccgcg
    24301 tcagtgtgga cgatgctatt gcagcagatc agatatttga aatgttgatg ggggatcgag
    24361 tagaaccccg tcgtgcattc atcgaggaaa atgcacacta tgtgaagaac ttagatattt
    24421 aatcatgaag gaggaaaaag aatgagtgaa gaaatcagag aaaacatcca tgatgtcaat
    24481 ctaaccagcg aaatgaaaga ctccttcatc gattatgcga tgagtgttat tgtcgcacgt
    24541 gcacttcctg atgtcagaga tggtctgaaa cccgttcatc gccgtatttt atacggcatg
    24601 aacgaattag gtgtaacacc ggataaagcc cataaaaaat ctgccagaat cgtcggagat
    24661 gtcatgggta aataccatcc tcatggagac tatgcgatct atgagtcaat ggtacggatg
    24721 gcacagccat tcagctaccg ttatatgcta gtggatggtc atggaaactt tggttctgtt
    24781 gacggtgacg gtgccgccgc tatgcgttat accgaagcac ggatgagcaa aatcgctaca
    24841 gaaatgctgc gtgatatcaa taagaacact gttgatttcc aaagcaacta tgatgataca
    24901 gaaaaagaac cagttgtact acctgctcgc ttcccgaatc ttttagtgaa cggaacaacg
    24961 gggatcgccg taggtatggc cacgaatatc ccacctcata acttgtctga ggtagtagcg
    25021 gctatcgatt tgctgatgga taatcctgat gtgacgacca atgaattgat ggaagtatta
    25081 cctggaccag attttccaac aggtggtttg gttatgggaa aatcaggaat ccgaagagcg
    25141 tatgaaacag gtaagggttc tattaccgta cgtgcaaaag ttgagattac tgagatgcca
    25201 aatggtaaag aacggatcat cgtgacagaa ttgccttata tggtcaataa agcaaaattg
    25261 atcgaacgga tttctgaact gcacagagac aagcgtatcg aagggatcac agatttgcgg
    25321 gacgaatctt cccgtgaagg tatgcggatc gtcattgaca tccgtcgtga cgccagcgca
    25381 tctgttatct tgaataactt atataaattg acatccttgc aaaattcatt tggattcaat
    25441 atgttggcga tcgaaaaagg tgtgccgaaa gttttgagct tgaaacaaat tcttgaaaac
    25501 tatatcgaac atcagcgcga agtcatcaca agacggacag tctttgaaaa agaaaaagca
    25561 gaagcaaggg cacacatttt agaaggtttg cgtattgctt tggatcatat cgatgaaatc
    25621 attgcgatta tccgcgggtc taaatcagat gacgaagcaa aagcgacgat gatcgaacgg
    25681 ttcgatttat ccgatcgcca gtctcaagct attttagata tgcgtttacg ccgattgacc
    25741 ggtttggaac gggaaaaaat cgaaaatgaa taccaagaac tcttaaaact gattgaagat
    25801 ttaactgaca ttctggcaag accagaacgt gttacagaaa tcatcaagaa tgaattagca
    25861 gacttgaatc aacgatttgg tgataaacga cgcacagaat tactagtcgg tgaggtctta
    25921 agtctggaag atgaagattt aatcgaagaa gaagaaatcg ttattacatt gacaaacaat
    25981 ggttacatca aacgtttggc aaataatgaa ttcagagctc aacgaagggg tggacgtggt
    26041 gtccaaggaa tgggtatcca caatgacgac ttcgtcaaaa atctggtttc ttgttctacc
    26101 catgatacat tgttattctt tacgaataac ggaaaagtgt atcgggcaaa aggatacgaa
    26161 attccagaat acgggcgcac agccaaagga ataccaatta tcaatttact aggtatcgat
    26221 tcgagtgaaa gcatccaggc aatcattgcg gtgacgggag aagcagaaga aggccactat
    26281 ctcttcttta cgacgcgtca aggtacagta aaacgtactt ctgtcactgc ttttgctaac
    26341 atccgaagta atggtctgat cgcaattggt ttgaaagaag acgatgagct cgtcaatgtg
    26401 ctactgacag atggtcaatc taatatcatt attggaacac ataacggcta ctctgtcact
    26461 ttcaaagaag aagcagtccg cgatatggga cgtacagctt ccggggttag aggaatacgc
    26521 ctgcgtgagg aagattatgt cgttggggca gccttaatga aggaagatca agaagtatta
    26581 gtgatcacag aaaaaggtta cggaaaacgg acaaaagtat cggaatatcc agtgaaaggc
    26641 cgcggaggta aagggatcaa aacggcgaac atcactgaaa aaaatggtcc actagctgga
    26701 ttgaccactg tttctggaga tgaagacatc atgctgatta cagataaagg tgtcattatc
    26761 cgtttcaacg tatcgactgt ttctcaaact ggacgttcaa cattaggcgt acgtttgatg
    26821 aagatggaag ctgatacgaa agttgtcacg atggcagcag tcgaaccaga agctgcagaa
    26881 caaacagttg aagaacaagg atcagaagaa tagccatcaa gactaattgt gtaagctagc
    26941 aagaatgacc gattaaaaaa tctggttatt cctgctggct tttttgatta gttgaatttc
    27001 tcttaaaata ttcgaaactg ttcattctgt attgcttttg accaaaatag agtgtataat
    27061 cagagcttgt gagtagtcta attaattaga taactctcct tgctcttagg aacctaagag
    27121 ccgaaagtcc acagggaggt gaatatcaat ggaaaacacg aaatatgaaa tcatgtacat
    27181 tattcgtcca aacattgatg aagaagcaaa aactgcttta atcgaacgtt tcgatgcaat
    27241 cttgaaagat aacggagcag aagttatcga atcaaaagat tgggaaaaac gccgtttcgc
    27301 ttatgaaatg aacggttacc gtgaaggtat ctatcacatc gttaaagtat cttctccatc
    27361 atcagcaaat gctgtcaatg aatttgaccg tcttgctaaa atcaacgacg atatcattcg
    27421 tcacatgatt gttaaagaag aagcttaatt gttgtttcat gtgaaacaat agaaaacgat
    27481 gagaggagat gactattttg attaataatg ttgtattagt tggacgtctg acgaaagatc
    27541 cagatttgcg ttacacagca agtggtacgg cagtcgcaac atttactttg gctgtgaatc
    27601 gtaattttac aaaccaaagc ggcaatcgcg aggctgattt tatcaactgt gtgatttggc
    27661 gcaaatcagc agaaacttta gcaaactatg ctcgtaaagg aacactttta ggtgttactg
    27721 gacgtatcca aactcgttct tatgataatc agcaaggaca acgtgtatac gtgacagaag
    27781 tagtagctga caacttccaa ttgctggaaa gtcgttctgc atctgaaaat cgccaacaaa
    27841 gcggcggatt ccaaagttct ggtcaatcag ctggcggatt tggcggaaac aataactcta
    27901 atcaaacatc tcaatcatcc aacggtatgc ctgatttcga ccgtgatact tctgacccat
    27961 tcggctcaag ttcaacgatc gacatttcag acgatgattt accattctaa acgaatttga
    28021 taggagggaa atagaatggc acaacaaaga agaggcggac gtaaacgtcg taaagtagac
    28081 tacattgctg ctaaccatat tgaatatatc gattataaag atatcgaatt actaaaaaga
    28141 tttatttcag aacgcggtaa aatcttgcca cgtcgtgtaa ctggtacagg cgctaaaaac
    28201 caacgtaaaa ttactgttgc aatcaaacgc gctcgtatca tgggcttgct acctttcgtt
    28261 agcgaagact aataaatcct aggaattgaa tgactgatcc ttttgatcag ctattcaatt
    28321 cctttttttc tgtaaaaata acctctattc gtttagtcgc tcgaatgacg aaagttatgg
    28381 taaaatcaaa ttagtcatat atgggaggaa aattatggaa aataaaaaca atcatttatc
    28441 cccgaaacct ctcttcctta tcctgtttat attaatcgaa gtattggtag tcttaatttt
    28501 accatttcgt tttgttagcg cttcggtcat tctattgatg aatttatggg gtgttcgcta
    28561 ttggttgaaa atgaaacgtt ggcttatttt aaataaacgt gaaaaaatcc agcaggcttc
    28621 gactgtagct gaaaccaatc tagcttatgt ctcagaagtc atgcctgtag gagtgattgc
    28681 ttatcagcca tcaagccaaa aaatcgaatg gctcaatcca ttagcattga aaatcaaaga
    28741 tcaaagtgga ttaacagagg aagaattgct agatactttt tttactgcaa aagaaaaaaa
    28801 gaaaaaccaa gtctatctag cagataaaac atttcagttt gatatggata cagtaaaggg
    28861 tgttgtttat tttaatgata ttacccaaaa ccagttgtta aaactccatc agacgaacag
    28921 tcagccggtg attggtattt tatctattga taactatgca gatgccattg ataaattgga
    28981 tgacaaagag atttcttatt tgaactcttt cgtcactaca ctagtttctg actggatgaa
    29041 tgaataccat gtctttttca aaagaatcaa cgcagaacgg tttttcttcg tagcacagat
    29101 gatcgatctg aaaaagatga tggataaaaa atttgatttg ctggatcgct ttcgagcaga
    29161 agctgaaaaa cagagctcgc ctatcaccct tagtatgggg attgcttatg gctcaagaac
    29221 cctcgatgaa atcggtggag aagctcagaa taatttagat gttgctttag tccgtggagg
    29281 cgaccaagtt gtcgtgaaag aggtaaaaga aggagcaaaa cctgtttatt atggggggaa
    29341 atctgctaag aatgtcaaac gcacgcgtgt tcgttctcga gcgatgagta ccgctttgcg
    29401 tcggattttt gatgaaacag gcgatgtgtt tatcatgggg cacaagtttc cggatatgga
    29461 tgctttaggc tctgcttttg gtgtcagttg tctggcacgc ttcaaccaga aaaaagctta
    29521 catcgttatc aacgagaacg agatcattcc agatgtagaa cggtgtcttg aagaaatcca
    29581 taagcatcct gaattagaaa tgcaactaat ctctccagaa cgtgccatag agctgaaaaa
    29641 agaagaggac ctcctcgtga tggtggatta ccataaacct tctctatcga tctctcaggg
    29701 cctatatgag caatttgata agattgtgat cattgatcac catcgtcgtg gggaagagtt
    29761 tccaagtaaa cctctgctga cttatatcga atcttcagct tcttcagctt cagagctagt
    29821 ttctgagctg atccaatacc aaagcagcaa aacaaatcgc ttagatcgtt ttgaggccac
    29881 attattgctt gcagggatgg ttgtagatac aaatagtttc aatgtccgta caactgcacg
    29941 aacttttgac gttgccagct atttaaggtc ttgtggagca gacgcatcct tagtacaata
    30001 tttattgagc tcggatttag cgacatactt ggagatcagc aatttgattg ctggaagcga
    30061 ctatgtgact tctgatattg ttgtggcggc agggacagac gaaaaagagt ataatagtgt
    30121 tatagcagca aaaacagcag atacattgct ttcgatgatc aatatcaatg cagctttcgt
    30181 cattacaaag cggacagacg gcttgattgg gatcagtgcc agaagtaccg gcacgatcaa
    30241 cgtccaaatc atcatggaat ccttaggtgg cggaggacac ttcacaaacg cagctactca
    30301 actggaaaat acgacagttg aggaaacaaa acagaagcta tttgctgcaa tcaaagaaaa
    30361 tatgaatcaa atatatgata aggagtaatc caaatgaaag taattttttt acaagatgta
    30421 aaaggaaaag gtaaaaaagg ggacatcaaa gaagttccag caggttatgc acaaaattat
    30481 ctaatcaaaa ataacttggc aaaagaagca acgaaaagca gtatcagtca gttagcagga
    30541 caaaagaaag cacaagaaaa acacgaagct gaaatcttgg aagaagctaa aaagttaaaa
    30601 gaattcttgg aaaaagaaga tactgtagtc gaattgaagg cgaaagcagg ggaagacagc
    30661 cgtttgtttg gttcgatccc ttcaaaacaa attgctgaag ggttaaacaa acaatacaaa
    30721 atcaaattag ataaacgaaa aatcgaatta gaaaatccga tccgttcact aggctacaca
    30781 aacgtacctg ttaagcttca tcatgaggtg acggctacga tcaaagttca tgtcgtacaa
    30841 gaataacaaa tcttgatagc gatgttcagc tggtaagtat aaccagaagg aaaaaagaca
    30901 aaccgaaatt tatctgagaa aacagctgca gcaatgaaag caaaagctgc aaatcgattt
    30961 cagattgatt tcggtttctt ttttgggaac agcaaaatag cataaagaaa acgagtataa
    31021 taaaagagaa ctattttttt attacacttt cggtacgaat gatgactttt ctctcatttt
    31081 cttgtaaaat gaagcaattg aagacaagga gcgatacgta tgaatgaagt gtggcaggat
    31141 cgaatcccac cgcaaaatat tgaagctgaa caagcggttt taggctctgt ttttcttgac
    31201 gcagaaacga tcattgatgc catggagtac atagagccaa aagactttta tcgtcgtggc
    31261 catcaaatta tttttgagac aatgatcgag ttaaatgatc gtaatgaagc gattgacgtg
    31321 gttactgtca aagaccggct ggaacaagcc aatttgttag aagatgccgg tggtttaagt
    31381 tatctctcag acttggcatt agcagttccg acagcagcaa atatcgtcta ctatgcgaaa
    31441 atcgttgaag aaaaatcatt gctgcgtaca ttgatccaga cagcaacagg aatcgttacg
    31501 aaaggattcg agcaaggaga agaagttcaa acaattttag atgatgccga acgcagtatc
    31561 ttggaggttt cggaaaaaag aaatcgaagc ggctttttat ctattgctga agtactgaac
    31621 tccacgattg aaaatatcga tcgactagct caaaacaatg aagaaatcac aggtctacca
    31681 actggctatc aaggtctgga taaaatgaca gcaggtttcc agcccgaaga actaatcatt
    31741 cttgctgcac gtccagcggt aggtaaaaca gcgtttgcat tgaatattgc tcaaaatgta
    31801 ggaacaaaaa cagatcgttc tgttgcgata tttagtttgg aaatgggggc agaatctctt
    31861 gtaaatcgga tgctttgtgc agaaggttcg attgaggcca gccatttacg tacaggtcag
    31921 ctttcggaag aagaatggtc gaatctgatc gttgcgatgg ggagtctttc tcgagcaaat
    31981 atttttatcg atgatacacc agggatcaaa atctctgaaa tccgtgcgaa atgtcggaaa
    32041 ctagcgcaag aaaaaggcaa tctaggcttg atattgatcg attatttgca gttgattgaa
    32101 ggtaacggta aagagaaccg tcaacaagaa gtatcagata tctctcgcca actgaaaaaa
    32161 ctggcgaaag agctgaaagt acctgtcatt gctctttcac agttatctcg tggagtagaa
    32221 cagcgacaag acaaacgacc agtgctaagt gatattcgtg aatccggatc aattgaacaa
    32281 gatgcggaca tcgtagcctt cttgtatcga gatgattatt atgaaagaga aggcggagat
    32341 gacgatgatg gaaacgagcc aaaaaatgac aacgtcatcg aagtcatcat cgagaagaac
    32401 cgaagcggtg cacgaggaac agttgaacta ctgtttatca aagaatacaa taagttttcg
    32461 tcgctttcgc caagagaaga attttaaaga agaggttgtg agccttgagc aatcccgaga
    32521 gctctcgtaa gaagatgcaa ggagtaaccg caggaacgac tagagtactt aatttaaaca
    32581 gatctccaaa gggattgtga caaaagtctt gtcacagtcc ctttttccga ataaacggtg
    32641 ttcaatcagc tgacccttgg tattagctca gcaaacgtca aaatatgagg aactattttg
    32701 acgtttgttt ctctaatact caggtcaaaa cgctgatctt acgacctctt ttccttaaac
    32761 ggtgttctcc tgccaactcc tcggtcacaa gtcacaatat catcaaacat gagaagcagt
    32821 tttttgaaat tgctccttgt gactcagagt taaacggcag gctcacaacc tctttcttaa
    32881 acggtgttca aaagcttgca tctgcggtaa tacgcccaat caaacgaaaa atatgagaag
    32941 ctatttttgt ttggctgttc ttattacttg atgcaggaca gctttttcac aacctctgat
    33001 tcacggtgtt ccaaaagtag cttcttcgaa aaataagtcg aaatttcaca aaaatttgag
    33061 aagcaatttt cgtacattcc ttcttatttc ttgaagctga ccacttctgt tacaacctct
    33121 tttttaataa ccattctaaa aagataaagt tcgtttttta agcgttttta taaaataaaa
    33181 ataaaaatca ttcgatttta tttgaaatat gtatctatta ttgatacaat ggttcaggat
    33241 taaataatat agaacgaggt gttcacatgt catctgtagt agtagtaggt acgcaatggg
    33301 gcgatgaagg gaaaggaaaa atcacagact ttctcagtga aaatgctgaa gtgattgcac
    33361 ggtaccaagg tggcgacaat gcagggcaca caattaagtt taacggtgtg acttataaac
    33421 tgcatttgat tccttctggg attttttata aagacaagat cagtgtgatc ggaaacggcg
    33481 ttgtagtcaa tcctaaatca ttggttaaag aattagctta tttacatgaa aaaggtgtag
    33541 aaacaagtaa tttgcgtatt tctgaccgcg cgcacgtaat cttgccttat catatcaaat
    33601 tggaccaatt acaagaagat gctaagggcg aaaataagat tggtacaacg attaaaggaa
    33661 tcggacctgc ctatatggat aaagcagctc gtgtaggtat ccgtattgca gacttattag
    33721 ataaagaaat ttttgaagaa cgtcttcgtt tgaatctaga tgacaaaaac cgtcaatttg
    33781 taaaaatgtt tgattcagaa ccacttgatt ttgacgatat ttttgaagaa tattatgaat
    33841 acggacaaca aatcaaacaa tacgtcacag atacttccgt tatcttgaat gatgctttgg
    33901 atgcaggcaa acgcgtgttg tttgaaggcg cacaaggtgt aatgctggat atcgaccagg
    33961 gaacttatcc ttttgttact tcttctaacc cagtagccgg tggggtaaca atcggaagcg
    34021 gtgtagggcc ttcaaaaatc aataaagttg tcggcgtctg caaagcctat acgtcacgtg
    34081 tcggtgatgg accatttcca actgaattat ttgatgaaac aggagaacaa atccgcgaag
    34141 taggacgaga atacggtacg acaactggtc gtccgcgtcg tgtaggctgg tttgattctg
    34201 tagttatgcg ccattctaaa cgagtttcag gtattaccaa cctatcttta aactcgatcg
    34261 atgtattaag tggattggaa acagtaaaaa tttgtacggc ttatgaatta gatggcgaac
    34321 taatctacca ctatccagca agcttgaaag agttgaaccg ctgcaaacca atctatgaag
    34381 aattgccagg ctggtcagaa gacattacag gatgcaaaac acttgcagaa ctgccagaaa
    34441 acgcacgtaa ctatgtacgt cgtatctcag agcttgttgg tgtacgtatc tcaacatttt
    34501 ctgtcggacc tgatagaaca caaacaaaca tcttagaaag cgtctgggcg caaatctaag
    34561 gataaaaacg acgactgaat caggacgttc ttaccagagc gagtcgaagt gtttatctat
    34621 acgataataa taacttgaac actgtttatt cagaataagg gactgtgaca aaaaccgtgt
    34681 cacagtccct ttttccgaa
//
LOCUS       DARBET010000024        34361 bp    DNA     linear   BCT 07-NOV-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4027
            SAMEA114569841-rid20112183.denovo.024, whole genome shotgun
            sequence.
ACCESSION   DARBET010000024 DARBET010000000
VERSION     DARBET010000024.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA114569841
            Sequence Read Archive: ERR12203666
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 34361)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 34361)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001972618.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001972618.1
            Genome Coverage       :: 132x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/07/2023 13:18:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,041
            CDSs (total)                      :: 2,975
            Genes (coding)                    :: 2,908
            CDSs (with protein)               :: 2,908
            Genes (RNA)                       :: 66
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 67
            CDSs (without protein)            :: 67
            Pseudo Genes (ambiguous residues) :: 0 of 67
            Pseudo Genes (frameshifted)       :: 26 of 67
            Pseudo Genes (incomplete)         :: 28 of 67
            Pseudo Genes (internal stop)      :: 20 of 67
            Pseudo Genes (multiple problems)  :: 6 of 67
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..34361
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA114569841-rid20112183.denovo.024"
                     /isolate="VRE4027"
                     /db_xref="taxon:1352"
                     /country="Denmark"
     gene            175..411
                     /locus_tag="SCJ86_001260"
     CDS             175..411
                     /locus_tag="SCJ86_001260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325335.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bacteriocin"
                     /protein_id="HEG1168834.1"
                     /translation="MFKHAKCKGTKYCGNAQTVGGGNNAWGKLGQVVGGLTTGAVGGA
                     GLGTAICGPACGVVGGLYGAVAGGAAAGWDARKK"
     gene            430..645
                     /locus_tag="SCJ86_001261"
     CDS             430..645
                     /locus_tag="SCJ86_001261"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294603.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Blp family class II bacteriocin"
                     /protein_id="HEG1168835.1"
                     /translation="MHIKNTKTTFILSSEELKNIQGGSAVGVLGTTFSGATAGVKLCS
                     AGGPYAIAACGVGGALLGAGFSMWTGA"
     gene            complement(1348..2100)
                     /locus_tag="SCJ86_001262"
     CDS             complement(1348..2100)
                     /locus_tag="SCJ86_001262"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325334.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HEG1168836.1"
                     /translation="MSYPIILCEDQIIQLNQLERIIDNFILFHDKVFKIVLKTQSPLE
                     VKKYLKQFRPKQGIYFLDIDLNHEVNGIELAEVIRKYDVQAKIIFTTTHDEMLPVTIK
                     RRVETLGFVTKDQTLDEYRNEIVELLLLAQERIDATKQVTNQAFVFSIGSQTFTFDIN
                     DIYFVEASKLPHRLSLCTKDGQYEFYGRISELEKKYPMLTRISRACLVNIFNVKEIDF
                     KKRSLYFDSELARNFTLGKAQKIKEKLKESTV"
     gene            complement(2115..3362)
                     /locus_tag="SCJ86_001263"
     CDS             complement(2115..3362)
                     /locus_tag="SCJ86_001263"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375903.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GHKL domain-containing protein"
                     /protein_id="HEG1168837.1"
                     /translation="MFVGNFFFFLLFYMTNTDLNKRKYLFVCLLIPLTFFVELFTDVS
                     AAIPIIGSYFILKEKKKTNIVLLNKLLLCMLIDDFRSIITSMVMTYMFSFEGIKGYGY
                     VVFQIMLDSLFLLMFIWLYRKLAINRIVEQSSSKLTAIFMIYLLTIGLFVSYVAHQYQ
                     VFDHFVFGVLIFLIIQMFVVLFLFIRITIRQREQYEQQLKRKELVYLKKYTDSLEKDQ
                     EKFARFRHDYKNLLLSLKEIANQNHDSILEKEIKELEIYSNSYLTNKFEYRYLKNIKN
                     EYLKSLLIAKLYQANNNKICCKFECPSTIIEVPVPIFDCIRVLGIILDNAIEASIESQ
                     DRELSLAIYRDDKQIEISISNSCREILLSIDTLMKKGITTKKGHQGLGLSSIEEINKK
                     NPNMFVNYQNDPYQFTTQVILLF"
     gene            complement(3438..3584)
                     /locus_tag="SCJ86_001264"
     CDS             complement(3438..3584)
                     /locus_tag="SCJ86_001264"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294600.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EntF family bacteriocin induction factor"
                     /protein_id="HEG1168838.1"
                     /translation="MEEKNRLNAKQCSDQELKKIKGGAGTKPQGKPASNLVECVFSLF
                     KKCN"
     gene            complement(3688..3999)
                     /locus_tag="SCJ86_001265"
     CDS             complement(3688..3999)
                     /locus_tag="SCJ86_001265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bacteriocin immunity protein"
                     /protein_id="HEG1168839.1"
                     /translation="MKKNAKQIVHELYNDISISKDPKYSDILEVLQKVYLKLEKQKYE
                     LDPGPLINRLVNYLYFTAYTNKIRFTEYQEELIRNLSEIGRTAGINGLYRADYGDKSQ
                     F"
     gene            complement(4001..4198)
                     /locus_tag="SCJ86_001266"
     CDS             complement(4001..4198)
                     /locus_tag="SCJ86_001266"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304799.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class II bacteriocin"
                     /protein_id="HEG1168840.1"
                     /translation="MKHLKILSIKETQLIYGGTTHSGKYYGNGVYCTKNKCTVDWAKA
                     TTCIAGMSIGGFLGGAIPGKC"
     gene            complement(4592..6319)
                     /locus_tag="SCJ86_001267"
     CDS             complement(4592..6319)
                     /locus_tag="SCJ86_001267"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315690.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease/substrate binding
                     protein"
                     /protein_id="HEG1168841.1"
                     /translation="MNNLLNYQLPVANWVENITEWFTTTFSGLFSFLQTIGQAVMSGI
                     TNLLLVIPAPLFILLLTVAAFFISKKRPGLTLFTLIGLWFIYNQGLWNDLMNTVTLVL
                     LSSVISIIIGVPLGILMAKSSKAQSIIKPILDFMQTMPGFVYLIPAVAFFGIGMVPGV
                     FASVIFALPPTVRFTNLGIRQVPKELVEASDSFGSTSRQKLFKLELPLAKSTIMAGIN
                     QTTMLSLSMVVIASMIGAPGLGRGVLSALQRAQVGNGFVNGVALVILAIIIDRFTQHL
                     NQPNNKKAAGATTQKSKKRQGLIIGAVVVVILGAIGIGSFSSAKETKRINLSYVEWDT
                     EVASTNVVGEVLKQMGYDVTMTPLDNSIMWKSVSNGESDAMVSAWLPKTHGSQYAQYK
                     DQVEDLGANLTGAKVGLAVPAYMDVNSIDELTDQAGKKIIGIEPGAGVVTAAENTIQK
                     YDNLKDWKVETSSSGAMTVALGQAIKKHEPIVVTGWTPHWMFAKYDLKYLEDPENGMG
                     SEEQIHTMVRKGLKEDQPEAYKVLDNFHWSEKDMEKVMLEINNGKDPQQAAKDWIKEN
                     QELVESWKK"
     gene            complement(6312..7505)
                     /locus_tag="SCJ86_001268"
     CDS             complement(6312..7505)
                     /locus_tag="SCJ86_001268"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734321.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine betaine/L-proline ABC transporter
                     ATP-binding protein"
                     /protein_id="HEG1168842.1"
                     /translation="MVKVEVKHLTKIFGKKTQAALDMMNDHQPKTEILKKTGATVGVY
                     DVNFDVKEGEIFVIMGLSGSGKSTLIRLLNRLIEPTSGSIYIDGEDVSKLSKEELREV
                     RRHKINMVFQNFGLFPHRTILENTEYGLEVRGVPKEERQEKAEKALENSSLLSFKDQY
                     PSQLSGGMQQRVGLARALANDPEILLMDEAFSALDPLIRREMQDELLDLQANVQKTII
                     FITHDLNEALRIGDRIALMKDGEIMQIGTGEEILTNPANDYVREFVEEVDRSKVLTAQ
                     NIMVPALTTNIESDGPNVALTRMRNEEVSMLMAVDRKRHLKGIITADQALEARKQKRP
                     LIDFLDENVTVIGKDMVVSDIFNIIYDSPTPLAVVEDGKLKGVVIRGSVIEALAETSE
                     VSEHE"
     gene            complement(7692..8336)
                     /locus_tag="SCJ86_001269"
     CDS             complement(7692..8336)
                     /locus_tag="SCJ86_001269"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319010.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HEG1168843.1"
                     /translation="MSGKKKIVRPRYQQIAVDLAERIVENRYKVGEKIHARSTLASTY
                     NASPETTRKAINVLVDLGIMEVRHGSGAFVASKEKAKDFLLQYQDVQSIQDTKAEIMA
                     SVEKQQDELNHFSQLLDQLVNQTKRIHKMNPLLPFELSLSEKAMHLDRSISELNIWQA
                     TGATVIAISHNEELLVSPGPYAKFTAGDIVHFIGNEYTLQRMTNFFYPEHQLAE"
     gene            complement(8430..8564)
                     /locus_tag="SCJ86_001270"
     CDS             complement(8430..8564)
                     /locus_tag="SCJ86_001270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290587.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FeoB-associated Cys-rich membrane protein"
                     /protein_id="HEG1168844.1"
                     /translation="MATFLLSVLIFGSAGAIVYTRLKSGKNCDDCQTACPVKKEQIKR
                     "
     gene            complement(8566..10698)
                     /gene="feoB"
                     /locus_tag="SCJ86_001271"
     CDS             complement(8566..10698)
                     /gene="feoB"
                     /locus_tag="SCJ86_001271"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629567.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferrous iron transport protein B"
                     /protein_id="HEG1168845.1"
                     /translation="MNIALVGNPNSGKTSLFNLLTGSNQYVGNWPGVTVEKKEGSLKK
                     NPNIHIQDLPGIYSLSPYTPEEVIARDYLVQEQPELIINIIDATNLERNLYLTTQLLD
                     LDIPMIIGLNMMDVIKKEGKKINLEKLSYGLGVPVLPISVLKKQGIEKLIEKIQESAK
                     KRPSIPVPCTYDPRLEAALHEIEDVLGEQSGKKRWYAMKYFERDQKVREDYEIAVSQQ
                     KEIEQLIQLTEKLLDDDSETILVNERYEFITQLCTLCVVSNDSFQLSMSDKIDQIATN
                     RWLALPIFAFVMWLIYYLAIQTVGTMGTDWINDTLFGTWLPEHVGRLLAEWQVAGWMQ
                     ELILNGIIAGVGAVLGFLPQLIILFLCLSFLEDCGYMARIAFVMDRLFRKFGLSGKSF
                     IPMLIATGCGVPGVMASRTIENEQDRRLTIMVTTFMPCSAKLPIIALVAGAFFPHQSW
                     VAPSAYFLGMTAIIFSGISLKKTRLFAGDTAPFIMELPAYHFPQWSAVLRQTYDRSKS
                     FVKKAGTIIFVSSIVIWFSSSYNFYAQPVSEDQSILAFFGRILAVVFQPLGWGNWQGT
                     VAAITGLVAKENIIGTFGILFGHAEVSENGREIWQVLQHTYTPAAAYSLLAFNLLCAP
                     CFAAIGAIHREMNAKKWTWIAIGYQCGLAYLVSFVIYQLGHLLEGGQVALGTYLAILL
                     MIGLVYVLFRQPKSKNQFYTILSLEGEE"
     gene            complement(10695..11168)
                     /locus_tag="SCJ86_001272"
     CDS             complement(10695..11168)
                     /locus_tag="SCJ86_001272"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferrous iron transport protein A"
                     /protein_id="HEG1168846.1"
                     /translation="MQTLAQAKQNFVYQVERIETEEKTKRHLQNIGIVAGSKIVVVNL
                     SGNNAILLVKSHRVAVSREILGQIFVNKKTASSESWTSLDQLKAGETGIVVAIHGQGA
                     VKRRLMDMGLTRNVKIVIRKLAPLGDPIELTVRGYELTLRKSEAELILIQREEKE"
     gene            11569..11793
                     /gene="nrdH"
                     /locus_tag="SCJ86_001273"
     CDS             11569..11793
                     /gene="nrdH"
                     /locus_tag="SCJ86_001273"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016625075.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutaredoxin-like protein NrdH"
                     /protein_id="HEG1168847.1"
                     /translation="MNVKIFSKNNCMQCKMVKRFLTENQIAFEEVNIDEQPEAVDWLK
                     AQGFQSVPVITSDAETVIGFRPDQLRKLAS"
     gene            11952..14111
                     /gene="nrdE"
                     /locus_tag="SCJ86_001274"
     CDS             11952..14111
                     /gene="nrdE"
                     /locus_tag="SCJ86_001274"
                     /EC_number="1.17.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310024.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class 1b ribonucleoside-diphosphate reductase
                     subunit alpha"
                     /protein_id="HEG1168848.1"
                     /translation="MSLKNLQDVSYFKLNNEINRPVNGQIPLNKDKEALVAFFEENVK
                     PNTMTFLSIMEKINYLIDQEYIEAEFIQLYSPSFIEKLYQFLDEQHFSFKSFMAAYKF
                     YSQYALKTNDGSAYLETYEDRVAFNALYFANGDEELALALADEMIHQRYQPATPSFLN
                     AGRKRRGELVSCFLTQVTDDMNSIGRSINSALQLSRIGGGVGITLSNLREAGAPIKGY
                     EGAASGVVPVMKLFEDSFSYSNQLGQRQGAGVVYLNVFHPDIIAFLSAKKENADEKIR
                     VKTLSLGVIVPDKFYELTRKNEDMYLFSPYSVEKEYGVPFSYVNITAEYDHLVANPNI
                     RKQKIKARDLENEISKLQQESGYPYVINIDTANRENPIDGKIIMSNLCSEILQVQTPS
                     VINGKQEYEVLGTDISCNLGSTNIVNLMQSPNFGQSVRTMTRALTFITDASDIDVVPP
                     IQNGNKLNHTIGLGAMGLHTYLAKEQMEYGSEDSLDFTNIYFMLLNYWTLVESNNIAR
                     ERKQVFHNFEKSAYADGTYFEKYVTGEFQPKSEKVKALFEGIFIPTAEDWNALKQAVM
                     KDGLYHQNRLAVAPNGSISYINDTSASIHPITRMIEERQEKKIGKIYYPAPYLSNETI
                     PYYTSAYDMDMRKVIDVYAMAQQHVDQGMSLTLFMRSEIPEGIYEWKTTTKQTTRDLN
                     ILRHYAFHKGIKSIYYVRTFTDDAEEIGSNQCESCVI"
     gene            14161..15126
                     /gene="nrdF"
                     /locus_tag="SCJ86_001275"
     CDS             14161..15126
                     /gene="nrdF"
                     /locus_tag="SCJ86_001275"
                     /EC_number="1.17.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016625077.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class 1b ribonucleoside-diphosphate reductase
                     subunit beta"
                     /protein_id="HEG1168849.1"
                     /translation="MSETYYEAINWNEIEDIIDKSTWEKLTEQFWLDTRIPLSNDLDD
                     WRTLSQLEKDTVGHVFGGLTLLDTVQSESGMDQLRKDVRTPHEEAVLNNIQFMESVHA
                     KSYSSIFSTLNTKKEIEEIFDWTNTNPYLQKKAERINEIYKHGTPLEKKIASVFLETF
                     LFYSGFYTPLYYLGNNKLANVAEIIKLIIRDESVHGTYIGYKFQLGFNELPEAEQETL
                     KDWMYNLLYELYENEERYTEELYDPIGWTEEVKTFLRYNANKALMNLGMDPLFPDTAN
                     DVNPIVMNGISTGTSNHDFFSQVGNGYLLGHVEAMKDDDYLIGLE"
     gene            complement(15215..16354)
                     /locus_tag="SCJ86_001276"
     CDS             complement(15215..16354)
                     /locus_tag="SCJ86_001276"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter"
                     /protein_id="HEG1168850.1"
                     /translation="MQSRFKQSKLFFWTVELVLTIIGVYFILQMPNIFSPILKMLSAV
                     FLPLLVAGFIYYMFDPIVVFLEKHRIPRVFGFLLTFLVVIILVVLTVMNVVPQLMEQT
                     IQLTQSLPMYADETSKWLNELAQREEFKNFNLEEQLASANLSLSNLFNIILVSLSASV
                     SKIISFMMQFFILLFTVPFILLFMFKDGHKFIDALSHFFPKAIRKELRQTVKELNETL
                     SAYISSTILDALIIGVMSFLAMRIFDQPYSLLLAVICGITNIIPYVGPFIGAVPAIIV
                     GLFISPFQALYMALSILVIQQLDGNLIKPLLFGKSMNIHPLTIILVLIGAGSVAGILG
                     MLICIPVYAVIKTIILNIRKIYLLRRMEHVSEKLDSPKVLEETSE"
     gene            complement(16663..17376)
                     /locus_tag="SCJ86_001277"
     CDS             complement(16663..17376)
                     /locus_tag="SCJ86_001277"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MgtC/SapB family protein"
                     /protein_id="HEG1168851.1"
                     /translation="MNDMVLTIPEIILRLGIAMLFGGAIGFERQYKNRPAGMRTHILV
                     CMGACIIALIQVEIASGALRDALEYPQLAGTIRSDEARLIAQVVSGVGFLGAGTIIVT
                     KQSVTGLTTAASLWAIAGLGIAIGMGYYSIAIISFIGIAIALTVVKRVIHVPTTKKLE
                     IQFIHRKETKDFLTDYFEEKNIVIEDVNFDVKFVDDYRVYKNIYTIDLPKGLTYADVI
                     EELSVHKNITKLHLVSIAQ"
     gene            complement(17630..18331)
                     /gene="nagB"
                     /locus_tag="SCJ86_001278"
     CDS             complement(17630..18331)
                     /gene="nagB"
                     /locus_tag="SCJ86_001278"
                     /EC_number="3.5.99.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucosamine-6-phosphate deaminase"
                     /protein_id="HEG1168852.1"
                     /translation="MEIIRVKNAEEGGKKAFEIIKEGMNKGAKVLGLATGSTPVTLYK
                     EMTSSDLDFSEMTSVNLDEYVGLGGSDEQSYRHFMDVQLFDKKPFKETFVPDGKAEDL
                     EAACKKYDQIIDAHPIDIQILGIGQNGHIGFNEPGASFDGTTSVVDLTESTINANKRF
                     FDKAEDVPTKAVSMGIGSIMKSKEIVLMAFGESKADAIKGMIEGPVTNHLPASVLQNH
                     DNVVVIIDEEAASKL"
     gene            18565..20097
                     /locus_tag="SCJ86_001279"
     CDS             18565..20097
                     /locus_tag="SCJ86_001279"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310001.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F family ATP-binding cassette
                     domain-containing protein"
                     /protein_id="HEG1168853.1"
                     /translation="MSILTLNNITQQFGDKILYEGVDLQLNAGEHLGLIGQNGAGKST
                     LIKIITGEILPDDGQIHWQKNIHKGYLDQYVEVDETLTIEEFLKTAYAKEYEKEEKIA
                     FLYQTYSETFDDSLLEKAGKLQTELDQGVFYQIDTLVAEMSSGLGIDVLGLDSLLKNL
                     SGGQRSKVILAKLLLENPDVLILDEPTNHLDDQHIQWLTQFLQDFDGAYIVISHDQEF
                     LDQITTHIADIEFGKITKYTGHLKQALKQKEQNRESYLRQYHAQQKQIEKTEAYIRKY
                     KAGSRSTMAKSRQKQLDKIERLTPPASASKAVFDFPFSPIVTTLAVETTDLVIGYDRP
                     LLAPINLTIRFGENIAIRGFNGIGKSTLLKTLIGEIEKISGDFHFPDNTKINYFSQDL
                     YWENPLETPLQYLSSQFPKATIKELRRQLAKAGLVNQLASEPLTTLSGGEQTKVKLAQ
                     LTMNQGNLLILDEPTNHIDQETKESLQDGIQKFPGTVIIVSHEQEFYQDLVQRVIEIE
                     PK"
     gene            complement(20288..21490)
                     /locus_tag="SCJ86_001280"
     CDS             complement(20288..21490)
                     /locus_tag="SCJ86_001280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEG1168854.1"
                     /translation="MKSNEKKPLTTSLILLLSITCGVVVANMYYIQPIGTKVAASLSV
                     STSAVGILTMLTQLGYALGLLFLVPLGDVVDRPKLIIRMAALSAVSLLAAFFAPSFTL
                     FACASFLIGLLSIVPQIIIPYGAVLAGPKARGKVMGTLLSGLLVGILLSRTVSGIVAS
                     LFSWRMIYLFALCAVGILTLLLYWKMPRSVQQSSTAVSYLDSLKSLPHLVASQRLLRE
                     AAVSGFFMFGTFSIFWSTLIFYISSPAYNWGTFEAGILAIFGLSGAVAAPIVGRLADS
                     YSERKIVYMGLVMQTISFIALLVGGSHLAILLLAIILLDVGNQFGQVANQTRVQGLGE
                     AASNRNNTVFMFMYFIGGAAGSLLGTTMWQQYGWLGVTVSGLVFQGCAFFFQTVVFKG
                     KRNLENKK"
     gene            complement(21572..22261)
                     /locus_tag="SCJ86_001281"
     CDS             complement(21572..22261)
                     /locus_tag="SCJ86_001281"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294589.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TVP38/TMEM64 family protein"
                     /protein_id="HEG1168855.1"
                     /translation="MKKKIIRLILISLGIILILVVGYRLYLEYQTDIQLFLNPRASKA
                     ELLAHIRSHGTFASILLIVLTAVMCAVPGIPTSVVGVIAGLSFGPLTGAIINILGNAL
                     GNITAIFLMHRLKFLDKKTETNHWVQAIRQMKHPKIGVMLGYMIPVIPSSMVNFAADA
                     MKLPLQQIILSIVIGVIPSSVLYACGGEALFHGYNKTAVTLVASVTVLVGLVVFIYKD
                     RKKKQGKQKHK"
     gene            complement(22367..22735)
                     /gene="rplL"
                     /locus_tag="SCJ86_001282"
     CDS             complement(22367..22735)
                     /gene="rplL"
                     /locus_tag="SCJ86_001282"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002356427.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L7/L12"
                     /protein_id="HEG1168856.1"
                     /translation="MALNIENIVAELKEATILELNDLVKAIEEEFGVSAAAPVAVAAA
                     GGAAAAEEQTEFTVELTAAGDQKVKVIKAVREATGLGLKEAKAVVDGAPAPVKEGVSK
                     EEAEELKAKLEEVGASVTVK"
     gene            complement(22788..23291)
                     /locus_tag="SCJ86_001283"
     CDS             complement(22788..23291)
                     /locus_tag="SCJ86_001283"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345549.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L10"
                     /protein_id="HEG1168857.1"
                     /translation="MSEAIIAKKAAIVDEVSEKFTAAASVVVVDYRGLTVDEVTRLRK
                     QLRDANVEMKVIKNSILSRAAKKAGLEGMDEVFTGPTAVAFSNEDVVAPAKIMDEFAK
                     EAKALEIKGGIIEGKVSSLEEITALAKLPNREGLLSMLLSVLQAPVRNVAYAVKAVAD
                     KEDGDAA"
     misc_feature    complement(23495..23622)
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00557"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="ribosomal protein L10 leader region; Derived by
                     automated computational analysis using gene prediction
                     method: cmsearch."
                     /db_xref="RFAM:RF00557"
     gene            complement(23701..24390)
                     /gene="rplA"
                     /locus_tag="SCJ86_001284"
     CDS             complement(23701..24390)
                     /gene="rplA"
                     /locus_tag="SCJ86_001284"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003127078.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L1"
                     /protein_id="HEG1168858.1"
                     /translation="MAKKSKKMQDALKKVDSSKVYPVAEAVALAKETNIAKFDATVEV
                     AYRLNVDPKKADQQIRGAVVLPNGTGKTQTVLVFAKGDKAKEAEAAGADFVGDDDMVQ
                     KIQNGWFDFDVVVATPDMMATVGRLGRVLGPKGLMPNPKTGTVTMDVTKAVNEVKAGK
                     VTYRVDKAGNIHVPIGKVSFDDEKLVENFAAIHDVLLKAKPSAAKGQYMKNVTITTTF
                     GPGIHVDQASF"
     gene            complement(24494..24916)
                     /gene="rplK"
                     /locus_tag="SCJ86_001285"
     CDS             complement(24494..24916)
                     /gene="rplK"
                     /locus_tag="SCJ86_001285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002356424.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L11"
                     /protein_id="HEG1168859.1"
                     /translation="MAKKVEKIVKLQIPAGKATPAPPVGPALGQAGINIMGFTKEFNA
                     RTADQAGLIIPVVISVYEDRSFTFVTKTPPAAVLLKKAAKIEKGSGEPNKNKVAKVSS
                     DQVREIAELKMEDLNAANVEAAMRMVEGTARSMGITVE"
     gene            25259..25927
                     /gene="sdaAB"
                     /locus_tag="SCJ86_001286"
     CDS             25259..25927
                     /gene="sdaAB"
                     /locus_tag="SCJ86_001286"
                     /EC_number="4.3.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299351.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-serine ammonia-lyase, iron-sulfur-dependent
                     subunit beta"
                     /protein_id="HEG1168860.1"
                     /translation="MKDLRYRSVFDIIGPVMIGPSSSHTAGAARIGKIIRSIFGEQPD
                     SVDIYLYESFAKTYRGHGTDIALVGGLLGMEPDDERLADSLKLAYEAGMEVLFVPKSE
                     KAEHPNSVKMLLKKGTRKLSVTGISIGGGNIQISELNGFKISLSMGTPTLIVVHQDVP
                     GMIAKVTSILSETNTNIGTMTVTRESKGENAIMIIEVDDPQVEETVKRLKQLPNIDSV
                     NYFD"
     gene            25940..26812
                     /gene="sdaAA"
                     /locus_tag="SCJ86_001287"
     CDS             25940..26812
                     /gene="sdaAA"
                     /locus_tag="SCJ86_001287"
                     /EC_number="4.3.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307112.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-serine ammonia-lyase, iron-sulfur-dependent,
                     subunit alpha"
                     /protein_id="HEG1168861.1"
                     /translation="MFLSIEELVDQAKAYPSVAELMIAVEMEMSGRSRSHIIEIMEKN
                     LSVMEQSINEGKEGVTSVTGITGGDAKKLDDYLNEADFLSGETILKAVRNAVAVNEVN
                     AKMGLICATPTAGSAGVVPGVLMAVRDRLDLTHEQQLEFLFTAGAFGLVIANNASISG
                     AEGGCQAEVGSASAMAAAALVCAKNGTPHQAAQAVAITLKNMMGLICDPVAGLVEVPC
                     VKRNALGSSQAFISADMALAGIESVIPPDEVVAAMYQVGRQMPSIFKETAEGGLAVTP
                     TAKRLAEEILNKRA"
     gene            complement(26903..27922)
                     /locus_tag="SCJ86_001288"
     CDS             complement(26903..27922)
                     /locus_tag="SCJ86_001288"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631920.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-hydroxy-acid oxidizing protein"
                     /protein_id="HEG1168862.1"
                     /translation="MEAGAFGYIRGGSEDEWTMKENTTSFNTKKIMPRILRGIDSADL
                     HTSVFGIELDTPIIQAPSAAQGLAHEKGEADTAKGVAAAGSIFSISTYANTTIKDAAD
                     AAPGAPQFFQLYMSKDDRFNEFILNKAVEAGAKAIILTADSTLGGYREEDVINQFQFP
                     LPMPNLAAYSEQSASGNGEGKGIAEIYAAAKQGLTPDDIKTIKEITHLPVIVKGIQSP
                     EDAVIAISAGADGIWVSNHGGRQLDGGPASFEVLPKIAEVVNKRVPVIFDSGVRRGEH
                     VFKALASGADLVAIGRPVIYGLNLGGAEGVTSVFEHLNKELSITMQLAGTKTIDEVKN
                     TKLMD"
     gene            complement(28095..29000)
                     /locus_tag="SCJ86_001289"
     CDS             complement(28095..29000)
                     /locus_tag="SCJ86_001289"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318099.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HEG1168863.1"
                     /translation="MERKRCMTEPIKNLCKRATAAALVLISLFALSGCQTTKINTTEV
                     SDKEKTAESSKVIEKTTIPTLFFHGYSGTENSFKGMLRRLEKNQQAVKELVLTVGTDG
                     QIQATGDLSGKADNPMVQVLFEDNKNNEWNQAEWIKTCLLYLKENYGIDKVNIVGHSM
                     GGVSALRYLGTYGQDTSLPQVQTLTAIGAPFNDFVDDSAQSLTDELAKGPAVVSNRYQ
                     DYQQMIGNIPITTRFFLIAGQLDETDLSDGTVPLNSALAVYALLKQRGNEIEEKVVTG
                     ENASHSMLHENQEVDQLVSRFIWKE"
     gene            complement(29059..29604)
                     /gene="nusG"
                     /locus_tag="SCJ86_001290"
     CDS             complement(29059..29604)
                     /gene="nusG"
                     /locus_tag="SCJ86_001290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294580.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination/antitermination
                     protein NusG"
                     /protein_id="HEG1168864.1"
                     /translation="MESFEKNWYVLHTYSGYENKVKANIESRAQSMKMADFIFRVVVP
                     EEKETEVKNGKEKEIVHKTFPGYVLVEMIMTDASWYVVRNTPGVTGFVGSHGAGSKPA
                     PLLPEEVNHILRSIGMSLRQNDLEVEVGEVVKIIEGAFAGLEGQVTEVDGEKEKLKVN
                     IDMFGRETSTELDFDQVDKID"
     gene            complement(29705..29875)
                     /gene="secE"
                     /locus_tag="SCJ86_001291"
     CDS             complement(29705..29875)
                     /gene="secE"
                     /locus_tag="SCJ86_001291"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019722316.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecE"
                     /protein_id="HEG1168865.1"
                     /translation="MKFLKSVKDEIKQVTWPSRKQLRKDTLVVIETSIIFGLMFYVMD
                     TAIQSLFGWILK"
     gene            complement(29899..30051)
                     /gene="rpmG"
                     /locus_tag="SCJ86_001292"
     CDS             complement(29899..30051)
                     /gene="rpmG"
                     /locus_tag="SCJ86_001292"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294578.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L33"
                     /protein_id="HEG1168866.1"
                     /translation="MAKKKAALACSVCGSRNYTKSVSEGKSGERLEINKFCKYCNQYT
                     LHKETK"
     gene            30213..30857
                     /locus_tag="SCJ86_001293"
     CDS             30213..30857
                     /locus_tag="SCJ86_001293"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008264881.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CBS domain-containing protein"
                     /protein_id="HEG1168867.1"
                     /translation="MLLKTLVYKKKDLTTVTETSTLEEALKILETSGYRCVPILDSSG
                     NIFRGNIYKMHIYRHKANGGDMTLPVTHLLKNATKFIYLDTSFFKVFFTIKELPYIAV
                     LDSENHFYGILTHSTLLNMLSQSWSVEQGSYVLTIASTGKQGDLAAIAKIISKHSSIA
                     SCITLDIEKDEYIRRTLITLPANTEAKVCETIVNHLEKKNYKVVEIENLQNQEA"
     gene            complement(30913..31416)
                     /locus_tag="SCJ86_001294"
     CDS             complement(30913..31416)
                     /locus_tag="SCJ86_001294"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318553.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="QueT transporter family protein"
                     /protein_id="HEG1168868.1"
                     /translation="MQKTKSRQRLSVIVVNGIIMALYLALTILVAPVSSGPIQFRISE
                     SLNHLVVFNRKLLWGVLGGVIVYNAFFGFGIMDVVFGGGQTLLSLGLTALLQNKVKNV
                     KMRLVLNTIFFTISMGLIAYMLVPTGGQAFWTTYGTLALSEFIVMAVSAPLMYYLDRA
                     LDFEKRV"
     regulatory      complement(31429..31475)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00522"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="PreQ1 riboswitch class I; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="preQ1"
                     /db_xref="RFAM:RF00522"
     gene            32013..32978
                     /locus_tag="SCJ86_001295"
     CDS             32013..32978
                     /locus_tag="SCJ86_001295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327280.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diacylglycerol kinase family lipid kinase"
                     /protein_id="HEG1168869.1"
                     /translation="MKFHYHILINPSAGSGNGYKVAERILPVLKNKHIDYTLHYSEYK
                     GHDAEIAETLAKETLIPWIEEEQKTIDTFPLLIIVGGDGTLHQVLDTFYQMEVEFPVA
                     YIPAGSGDDFARGAGLPKNPKKGLQLILAAQSPEKVHIMAYEEKISEKKGIAVNNFGI
                     GLDAAIVHATNHSSTKKRLNKYNLGSFSYLFSILRALFTQKGFPILVDAGGKQLSFSN
                     AFLCTATKHPFFGGGIAIVPTADASKPVIDFVVVERINFFKILWLILLLIQKKQLKSK
                     YFHHYTTNRLRIVSTTPQHGQEDGEEMEKRPFDITITTTKQLFWC"
     gene            complement(33077..34249)
                     /locus_tag="SCJ86_001296"
     CDS             complement(33077..34249)
                     /locus_tag="SCJ86_001296"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628387.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEG1168870.1"
                     /translation="MTLYKTIWTLLKANKSNLLIGVIITVVATFFFAQNIDVENTSLQ
                     DAKIALISEEESPVVDGLRKYLNDKETVVTLDGTSQKEIDDALYFNRVNIILHLPADF
                     TEQVEVGKMPTIKIQSRPDAFSKTVVTQQVNTFLQTLILFQEEDKTIDAAMEQTQMAL
                     SVTGEVELSAGYSQRMKKLLTGTTFNFLSYGLFLSIFSGFSVINLAFNRKEISKRNQS
                     APITKRNLNRKVTFSLISYSLLLFSGFLLLMVLLVIHTSWDITVWYHILNTFLFLLPI
                     ISFSACITSLVKNSETSGGIKDIFITGSCFIGGVFVPAEYLPEMVSKIAAFTPTYWFV
                     QNNNLISETLSYNQTFAESFWFNGCVLVAFAAVFSMIQFILGKERNYRWQPNWAKN"
     gene            complement(34236..>34361)
                     /locus_tag="SCJ86_001297"
     CDS             complement(34236..>34361)
                     /locus_tag="SCJ86_001297"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294551.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEG1168871.1"
                     /translation="KLYFYTDLASFYTNLLWLIGFIFLFALGNAWMERGVRYDAL"
BASE COUNT        10624 a         6875 c         5957 g        10905 t
ORIGIN      
        1 ttccacaaaa actttgaaaa cagttcttga acactaactt tttcattcct gaatcaaatt
       61 tattttggaa attttactat tcaagaataa acttgataag tttcaggaag atttttactg
      121 ttaatcaaat acgtatgtta cagtagcacc gtaaaaaata ttatttaaag aagcgtgttt
      181 aaacatgcaa aatgtaaagg aactaagtat tgtggaaatg cacaaactgt tggtggaggc
      241 aataatgctt ggggaaaatt ggggcaagtt gtaggtggac ttaccacagg ggcagtaggt
      301 ggtgcaggtt taggaacagc aatttgcgga ccagcttgcg gtgttgttgg agggttatat
      361 ggagctgtcg ctggaggtgc tgcagctggc tgggatgctc gaaaaaaata aaaaaggaga
      421 ataagtagta tgcatattaa aaacacaaaa acaacattta tattatcttc agaagagttg
      481 aaaaatattc aaggtgggag tgccgtggga gttttaggca ctactttttc tggggcaaca
      541 gcaggagtaa aactttgttc agccggagga ccttatgcaa tagctgcttg tggtgtaggt
      601 ggagctttat taggtgctgg attcagcatg tggaccggtg cgtaaacaat taaaaaatat
      661 caaacctcaa gattggtttg tgtcgattac gaacataggt atgttcttta tcttttcaaa
      721 agtttggaat ggacagaact ttttactaaa gcttcttgtg ctagttttta taattatatt
      781 aaatttttat cttttattaa agtcagataa aaattctttg gaaatgtaat ggtagagata
      841 tttttacaaa aggacttgca gcgctgtagt tggtgctgca ggaggaccta tttcggccgt
      901 tacaggaggt attggtaatg tacttgtcgg atgtgcatta aagcctgttc ctgcaagtta
      961 aatttataaa aacaggaaag taattatctc aaatcctatc aagtacaatc ataggattgc
     1021 acttgacagg acaaattttt taactaaaaa atgaaaaaca ctttctaaaa attagtagaa
     1081 tttgccaaat ctttcgcatc ttccaccata catttaattt taattcccct ttatggtact
     1141 taataaaaac aagaaaagaa ctattcattt ggcccttttt tggttccaga ccatagaaat
     1201 attcctccag taactaatat tgctgttatg cacgtaacta ataacagcta taaaaattgg
     1261 tacaacttta ataggattca gaaaaatttg cactagtgaa ggaatctatt ttattagaaa
     1321 aatgcaatct ccctaaagaa aaaaccacta aacagtgctt tctttcaact tctccttaat
     1381 tttttgtgct ttccctaaag taaagtttct ggccaactct gaatcaaaat atagtgaacg
     1441 ttttttaaaa tctatttctt taacgttaaa gatatttact aaacacgctc gactaattct
     1501 tgttaacata ggatatttct tttcaagctc actaattctt ccataaaatt cgtactgccc
     1561 atctttagta cacaatgata atctatgtgg taattttgaa gcttctacaa aataaatgtc
     1621 atttatatca aaagtaaatg tttgagaacc tatggaaaaa acaaatgctt gattcgtaac
     1681 ctgttttgtt gcatctatcc tttcttgcgc taataacaat aactcaacaa tctcgtttcg
     1741 atactcatct agtgtttgat cttttgttac aaatcctaac gtttcaactc ttctttttat
     1801 tgttacgggt aacatctcat catgagtagt tgtaaaaatg atttttgctt gaacatcata
     1861 ttttctgatt acttctgcta attctatacc gttaacttca tgatttaaat caatatctaa
     1921 gaaatatatt ccttgctttg gtcggaattg tttgaggtat tttttaactt ctaacggact
     1981 ttgtgttttc aatactatct taaatacttt atcgtgaaaa agaatgaaat tatcaataat
     2041 tcgttctaac tgatttaatt gtataatttg atcttcacat aatattattg gataactcat
     2101 aaatcctcct tccctcaaaa caacaaaata acttgagtag taaattgata cggatcgttt
     2161 tgatagttca caaacatatt gggattcttt ttattgattt cctctatact agataaacct
     2221 aacccttgat gtcccttttt tgttgttatt ccttttttca ttagagtatc tatagagagt
     2281 agtatctctc tacaactatt cgatatcgaa atttctatct gtttatcatc tcggtaaata
     2341 gctagagaca gttctctatc ttgactctct atagatgctt caattgcatt atcgagaata
     2401 attccaagca ccctaataca gtcaaatatt ggaacaggaa cttcaataat agtacttgga
     2461 cattcgaatt tacaacaaat cttattgtta tttgcttggt atagcttcgc tatcaacaaa
     2521 cttttcaaat attcattttt tatatttttt aaataacgat attcaaactt attggttaga
     2581 taagaattag aatagatctc taattctttt atttcttttt ccaatatcga atcatggttt
     2641 tgattagcga tttcttttaa actcagcaat aaatttttat agtcatgtct aaatctggca
     2701 aatttttctt gatctttttc cagtgaatca gtatattttt ttagataaac caattctttt
     2761 cttttcaatt gctgttcata ttgttctcgt tgcctaatag ttattctaat aaataaaaat
     2821 agaacaacaa acatttgaat gattaaaaat attaagacac caaacacaaa atgatcaaat
     2881 acttgatatt gatgagcaac ataagaaaca aataaaccaa ttgtaagtaa ataaatcatg
     2941 aatatagcag taagtttaga actggactgt tcaacaatcc tatttatagc taattttcta
     3001 tataaccaaa taaacatcag caaaaacaaa gaatcaagca ttatctggaa tactacataa
     3061 ccataccctt ttatcccttc aaatgaaaac atatacgtca taaccatgga agtaataata
     3121 gatctgaaat catcaataag catacataat agtaatttat ttaaaagcac tatattagtt
     3181 ttcttctttt cctttaatat aaaataactt ccgattatag gtatagctgc ggaaacatca
     3241 gtaaataatt caacaaaaaa agtgagtggt atcaataaac atacaaaaag atattttctt
     3301 ttgttaagat cagtattagt catataaaat aagagaaaaa agaaaaagtt tcccacgaac
     3361 attcttaata gataagcttt tgtatcgata gtaatcaaga gatcccctcc tgattttagg
     3421 atatcataaa aaaaatttta gttacacttt ttaaataatg aaaaaacaca ttccactaaa
     3481 tttgatgccg gttttccttg aggttttgtt ccagcacctc ctttaatttt tttaagctct
     3541 tgatctgaac attgtttagc atttagtcta tttttttctt ccattacgat tccccctaca
     3601 aactataaat acttttatgt cactgattca atatacctat ttttctaatc aaaaaatttt
     3661 gagacatatt taatattata agtatcatta aaattgagat ttatctccat aatctgctcg
     3721 atataaacca tttattccag cagttcttcc aatttcactc aaatttctta ttaattcctc
     3781 ttgatattca gtgaatctta ttttattagt ataagcagta aaatatagat aattcaccaa
     3841 tctatttatt aaaggaccgg gatctaattc atatttttgt ttttctaatt ttaaatatac
     3901 cttttgtaaa acctcaagaa tatcagaata tttaggatct ttacttatag atatatcatt
     3961 atataattca tgaacaattt gcttagcatt ttttttcatt ttagcacttc cctggaattg
     4021 ctccacctaa aaaaccacct atagacattc ctgcaataca agtagttgcc ttggcccaat
     4081 cgaccgtaca tttattttta gtgcaataca ctccatttcc ataatatttt ccactatgag
     4141 tggtaccccc atagataagt tgtgtctctt taatagacaa aatttttaaa tgtttcataa
     4201 tctaatcacc cctaaattaa tgtttttaga ctattattct aacattcatt aaatttcaaa
     4261 aaacaattcc ttcttgaaac gtagcaaaat aattcctgaa taagaattta atcactttta
     4321 agtatataaa ctatgtatat tctttaatat tttttgagaa tgcacttcgt acataataaa
     4381 ctttaatcac attagtcttt catacctacg atcgatctct ttgactcgca cccataaatt
     4441 cataatagtt tgtcggcgtc tttggacgtt tcgaagatga gctacgcatt ctcttttttt
     4501 atagacaaaa aagatagaga cgaacatgga gcttccaaat ttggatattt gatccaattt
     4561 tggaagctcc atttatttcg tgtctgtctt tttatttttt ccagctttcg actaattctt
     4621 ggttctcttt gatccagtct tttgctgctt gctgtggatc ttttccattg ttgatttcca
     4681 acataacttt ttccatatct ttttctgacc aatggaaatt gtctaatact ttgtaagctt
     4741 ctggctggtc ttctttcaat ccttttcgaa ccatcgtatg gatctgctct tcgcttccca
     4801 ttccgttttc aggatcttct aagtatttca gatcatattt ggcaaacatc caatgaggtg
     4861 tccatcctgt aactacaatt ggttcgtgtt ttttgatcgc ttgacctagt gcaacggtca
     4921 ttgctcctga agaagatgtc tctactttcc agtctttcaa gttgtcatat ttttggatcg
     4981 tgttttcagc agccgtaact acaccagctc ctggctcgat tcctatgatt ttcttacctg
     5041 cctgatcggt taactcatcg atcgaattta catccatgta ggctggcact gctaaaccaa
     5101 cttttgctcc tgtcaaattg gcacctaggt cttcgacttg atctttatat tgtgcatatt
     5161 gtgaaccatg agttttaggt agccaagctg aaaccatggc atctgactcg ccatttgaaa
     5221 cagatttcca catgattgaa ttatctaatg gcgtcatcgt tacatcatag cccatttgtt
     5281 tcaatacttc accgactaca tttgttgaag caacttctgt atcccattca acataagata
     5341 agttgatacg ttttgtttct ttggcagatg aaaaactccc tataccaatc gcacctaaga
     5401 tcactactac tacagcacca atgatcagtc cttgacgttt tttacttttc tgtgttgttg
     5461 ctcctgcagc ttttttattg tttggctggt tcaaatgctg tgtaaaacgg tcaatgatga
     5521 ttgctaagat aactaatgca accccgttga caaatccatt cccgacttgt gctctttgca
     5581 atgctgataa tacgccgcgt cctaagccgg gtgcaccgat catagaggca ataacgacca
     5641 ttgaaagtga cagcattgtt gtttgattga tccctgccat gatcgtactt ttagctaaag
     5701 gcaattctag tttgaatagt ttttgacgac ttgtgctccc gaatgaatca gaagcttcta
     5761 ctaattcttt aggaacttgt cgaatcccta aattcgtaaa acgaacggtt ggtggcagag
     5821 caaagatgac tgaggcaaat acccctggaa ccatcccgat accgaaaaat gcaactgctg
     5881 gaatcaaata aacgaatcct ggcattgtct gcataaagtc aaggatcggt ttgataatac
     5941 tttgggcttt actgcttttt gccatcaaga tccctaatgg taccccaatg atgatcgaga
     6001 tcacactaga taaaagaaca agtgtgacag tattcatcaa atcattccat agaccttggt
     6061 tataaataaa ccatagaccg attaatgtga acaacgtaag tcctggacgt ttcttagaaa
     6121 taaagaaagc tgctaccgtc aataacaaga taaacaaagg agctggaatc actaacagta
     6181 aatttgtaat ccccgacatc actgcctgtc caatcgtttg taaaaatgaa aataggccag
     6241 aaaaagttgt cgtaaaccat tctgtgatat tttctaccca gtttgccact ggtaattgat
     6301 agttcaatag attattcatg ttcactcacc tcgcttgttt ctgctaatgc ttcaatcaca
     6361 ctacctcgga tgacgacacc ttttaatttt ccatcctcta caactgcaag tggagtcggt
     6421 gagtcataaa taatattgaa aatatcactt accaccatgt ctttcccaat caccgtcaca
     6481 ttttcatcta ggaagtcgat caatggacgt ttttgcttcc tagcttctaa cgcttgatct
     6541 gcagtgatga tccctttcag gtgtctcttt ctgtcaacag ccatcagcat gctgacttct
     6601 tcattacgca tccttgtcaa tgcaacgttt ggtccatcag attcgatatt cgtcgtcaat
     6661 gcaggtacca tgatgttttg agccgtaagc actttggaac gatcgacttc ttccacaaat
     6721 tcacgtacat aatcatttgc tggattggtc aatatttctt cacctgttcc gatctgcatg
     6781 atttcgccgt ctttcatcaa ggcgattctg tccccaatcc gtaaagcttc attcaaatca
     6841 tgggtaatga aaataatcgt tttttgaaca ttggcttgca ggtccagtaa ttcgtcttgc
     6901 atttctcgcc ggatcagcgg atcaagagcc gagaacgctt cgtccatcag caagatctca
     6961 ggatcatttg ctaaagctct ggctaggcca acccgttgtt gcatcccacc agataactga
     7021 ctaggatatt ggtctttaaa tgaaagcaaa ctagaatttt ctaatgcttt ttctgctttt
     7081 tcttgtcgtt cttctttagg tacaccacgg acttctaacc cgtattctgt attttccaag
     7141 atcgttcgat gtggaaacag accgaagttt tggaaaacca tattgatttt atgacgacgg
     7201 acttctcgca attcttcttt gcttaacttc gatacatctt ctccatcaat atagatactg
     7261 cccgaagttg gctcaatcag acgattcaat agacggatca atgtggattt cccactcccg
     7321 gaaagcccca tgatcacgaa gatttctcct tcttttacgt caaaattcac gtcatacaca
     7381 ccaaccgttg caccggtttt ctttaaaatc tccgttttcg gctggtgatc attcatcata
     7441 tctaaagcgg cttgtgtctt tttaccaaat atctttgtta aatgttttac ttctactttt
     7501 accaaacaaa ttctcctttt ttatgtaaat tcatttttct ttctgaaaaa agtgaccaca
     7561 ctatgttaac atcatgtagt cactttgtca aaaatgaaag aaatttgctt gtccaaactg
     7621 aaaaaaactt ggctaaatct ttgtaaataa aaggtttagc caagttaata tttttttaat
     7681 atttcacaca atcattctgc aagctgatgc tccgggtaaa aaaagtttgt catacgctgt
     7741 agtgtatatt catttccaat aaaatgaacg atgtcccctg ccgtaaattt agcatagggg
     7801 ccaggtgaaa caagcagttc ttcattatga gaaattgcga taactgtggc gccagtcgcc
     7861 tgccagatat tcaactcgct aattgaacgg tccagatgca ttgctttttc tgataaagat
     7921 aattcaaacg gaagtaaagg attcatctta tgtattcgct tcgtttgatt gaccagctga
     7981 tccaataatt gagaaaaatg attcagctca tcttgttgct tctccacact tgccatgatt
     8041 tctgcttttg tatcttggat ggattgtaca tcttgatatt gcaataggaa atcttttgct
     8101 ttttccttcg aagcaacaaa tgccccactt ccatgccgta cttccatgat tccaagatct
     8161 accagcacat tgatagcctt gcgggtagtc tcaggagaag cattgtacgt actggcaagt
     8221 gttgagcggg catggatctt ctcacccact ttatatcggt tttcaacgat acgttcggct
     8281 aaatcaacag caatctgctg gtagcgcggg cggacgattt ttttctttcc agacattttc
     8341 tttcctcttt ctttttttac tttccctcat cttactatgc tctagcaaaa taagcaaaaa
     8401 aggggagaat agcctcttgc aacgactgat catcgcttga tctgttcctt ttttactggg
     8461 caagctgttt gacagtcatc acaattctta ccggatttta aacgagtata gacaattgcg
     8521 cctgcacttc caaagattaa cacacttaat aagaatgtcg ccatcctatt cctctccttc
     8581 taatgacaat atagtataaa actggttctt ggattttggc tgacggaaca aaacataaac
     8641 gagtcctatc atcagtaaaa tagcaagata cgtccctaac gctacctgtc caccctccaa
     8701 taagtgtccc aactgataaa tcacaaaact aactaaataa gctaaaccgc attgatagcc
     8761 tatggcgatc caagtccatt tcttggcatt catttcccga tggatagccc caattgccgc
     8821 aaaacaaggc gcacaaagta aattgaaggc taacaatgaa taagctgctg cgggagtata
     8881 tgtgtgttgt agcacttgcc agatttctct gccattttcg gatacttccg catgaccaaa
     8941 caaaatacca aatgtgccaa taatattttc tttagcgacc aatcctgtaa tcgctgcgac
     9001 tgttccttgc cagtttcccc aaccaagcgg ctgaaaaaca actgccagga ttcgcccgaa
     9061 aaaggctaag atactttggt cttcagaaac aggttgagca taaaaattat aagaagagct
     9121 gaaccagatc acgatactag aaacaaagat aatcgttcct gcttttttaa caaaagactt
     9181 gctgcggtca taggtttgtc ttaggaccgc tgaccattga ggaaaatgat atgcgggcaa
     9241 ttccatgata aaaggagctg tatctccagc aaacaaacgt gtttttttca agctgattcc
     9301 agagaaaata attgcagtca taccaagaaa ataggcagaa ggagctaccc aagattgatg
     9361 aggaaaaaag gcccctgcta ctaaggcaat aatcggcaac tttgcagaac aaggcatgaa
     9421 ggttgtgacc atgatcgtca aacgacgatc ttgctcgttt tcaatcgtgc gactggccat
     9481 gacgcctggc actccacatc ctgtagcaat cagcatagga atgaaggatt ttcctgaaag
     9541 accgaatttc ctgaaaagtc gatccatgac aaaagcgatc ctcgccatat atccgcagtc
     9601 ttccagaaag ctcaagcata aaaacaatat aatcaattgc ggtagaaatc ctaacactgc
     9661 tcccacgcct gcgatgatgc cgttcaagat cagctcctgc atccatcccg ctacttgcca
     9721 ttcagctaac aatctgccaa catgttctgg taaccatgta ccaaacaatg tgtcattgat
     9781 ccaatctgtc cccattgttc ctactgtttg aatagccaga taatagatca gccacatcac
     9841 aaatgcgaag atcggcaagg ctaaccaacg attcgttgca atctgatcga ttttgtcact
     9901 catcgataat tggaaagaat catttgacac aacacagagt gtacatagtt gggtaataaa
     9961 ttcataacgt tcattgacca gaatcgtctc actatcgtcg tccaatagtt tctctgttaa
    10021 ctggatcagt tgctcgatct ctttttgctg agaaactgct atctcgtaat cctctcttac
    10081 cttctgatct cgttcaaaat atttcattgc ataccagcgt ttcttgccgc tttgttctcc
    10141 tagtacatct tcaatttcat gtaaagctgc ttctaaacgt ggatcatatg tacagggaac
    10201 aggaatagaa ggccgctttt ttgcagactc ctgaatcttt tcgattagtt tttcgattcc
    10261 ttgctttttc aatacgctga ttggtaaaac tggtacacca agaccatagg aaagcttttc
    10321 tagattgatt ttttttcctt cttttttgat gacatccatc atattcagac caatgatcat
    10381 aggaatgtct aaatccaata actgcgtagt taaataaaga tttcgttcta aattggtagc
    10441 atcaataatg ttgatgatca gttcaggctg ttcctgaacc aaataatctc ttgcaatcac
    10501 ttcttctggt gtataaggag acaaagaata gatgccgggt aggtcctgaa tatgaatgtt
    10561 cggatttttt ttcaacgatc cttctttttt ttctactgtt acgcccggcc aattccctac
    10621 atattgattc gaaccagtca agagattgaa caagctagtt ttcccgctgt ttggatttcc
    10681 gactaacgcg atattcattc tttttcctcc ctttgtatca agattagttc ggcctctgac
    10741 tttcgtaacg tcagttcata ccctcgcact gtcagttcga ttggatctcc cagaggggct
    10801 agttttcgga taactatttt tacatttcgc gtcaatccca tatccattaa ccgtcgcttc
    10861 actgcgcctt gtccgtggat tgctacgacg atcccagtct ctcctgcttt taattgatca
    10921 agagaagtcc agctttcaga ggaagcagtc tttttattga caaagatttg acctagtatt
    10981 tcccgactta ctgccactcg atgacttttt accagtaaaa tggcattgtt gcctgataaa
    11041 ttgacgacga cgattttact tccggcaacg atcccgatat tttgcaaatg gcgttttgtt
    11101 ttttcttctg tttcgattcg ttcgacttga taaacgaaat tctgctttgc ctgcgctaag
    11161 gtctgcatat tgattcctct cttgtgaaat aaatcatgta ttaaaatcat tcattatatg
    11221 ttctaatttt cttacgtgtt acgcaaataa atgagaatca ttctcacata gaatactctc
    11281 ggtaattgag aatgtcaatt atacttgtga tattatgagc gaatctttag tattgataga
    11341 taaaaatgag aaaaaaatat ttcttcatag ttttccacaa ttttttctct attaaatttg
    11401 ctattttccc acacgatact tgttttcagc tcttttttca aaaacacaaa acttagtatt
    11461 ccacagtttc cacaacacaa tatgtagtgt ttgttgtttg acttttttac taagagtttt
    11521 atactatcta ttatagataa taagaatgtg ttagaggagg cactcaccat gaacgtaaaa
    11581 atcttttcta aaaataattg tatgcaatgt aagatggtca aacggttttt aacagaaaat
    11641 caaattgctt ttgaagaagt caatatcgat gaacagcctg aagcagtaga ctggttgaaa
    11701 gcacaaggat tccaaagtgt tcctgtcatt acttctgatg cagaaacagt tatcggattc
    11761 cgtcctgatc agttgcgtaa acttgccagc taacatgact aaaagaatca aaacgtgata
    11821 catggatttg aagggcttga gacagcacca gtttttgtcg caaacttcca aatccttttt
    11881 taaagcttaa aaacaatgca atgatttgca atcaagcatc atcaaatacg aaatgaaggt
    11941 tgggcgtacg tatgagtctt aaaaatttac aagatgtcag ttatttcaag ctgaataatg
    12001 aaatcaatcg gccagtcaat ggtcaaatcc ctttgaataa ggataaagaa gctttagttg
    12061 ccttttttga ggaaaatgtc aaaccaaata cgatgacttt tctatctatt atggaaaaaa
    12121 tcaattactt gatcgatcaa gaatatatag aagcagaatt cattcaattg tattcaccat
    12181 catttattga aaagctatat caatttttgg acgagcaaca tttttctttc aaatctttta
    12241 tggctgctta caaattttat tctcaatatg cgttgaaaac caacgacggt tccgcttatc
    12301 tggaaactta tgaagatcga gtagccttca atgcgcttta ctttgcgaac ggggatgaag
    12361 aattagcact tgctcttgca gacgaaatga tccatcaacg atatcagcca gctacacctt
    12421 ctttcttgaa tgctggacgt aaacgtcggg gcgaattggt gtcttgtttc ttgactcaag
    12481 tcactgacga tatgaactct atcgggcgct caatcaactc tgctttgcaa ttgtcccgta
    12541 ttggtggcgg cgtagggatt actttatcaa atcttcgtga agccggcgct cctatcaaag
    12601 gatatgaggg tgctgcaagt ggtgtcgttc cagtcatgaa attattcgaa gatagtttca
    12661 gctattctaa tcaattaggt cagcgtcaag gagctggcgt ggtttatttg aacgttttcc
    12721 atccagacat tattgctttc ctttcagcaa aaaaagaaaa tgcagatgaa aagattcgcg
    12781 taaaaactct ttctcttggc gtgatcgtac ctgataaatt ttatgaattg acacgtaaaa
    12841 acgaagatat gtatttattt agtccatata gtgtcgaaaa agaatatggt gtcccattct
    12901 cttatgtaaa tattacagct gagtatgatc atctagttgc aaatccaaac attcgcaaac
    12961 aaaaaatcaa agcgcgtgat cttgaaaatg aaatctcaaa attacaacaa gaatctggct
    13021 atccttacgt aatcaatatt gatacagcga accgcgagaa tccaatcgat ggcaagatca
    13081 tcatgagtaa tttatgttca gagattttac aagttcaaac gccttctgtg attaacggca
    13141 aacaggaata tgaagtatta ggaacggata tctcatgtaa cttaggctcg acaaatatcg
    13201 tgaatttgat gcaaagccct aactttggtc aatctgtgcg tacgatgact cgtgcattga
    13261 catttattac agatgcatct gatatcgatg tcgtaccacc gatccaaaac ggaaataagc
    13321 tgaatcatac gattggatta ggtgccatgg gcttacacac ttatcttgca aaagaacaaa
    13381 tggaatatgg ctcagaggat tcattagact ttacaaatat ttacttcatg ctgttgaatt
    13441 attggacact tgttgaaagc aacaatatcg ctcgtgaaag aaaacaagtt ttccataatt
    13501 ttgaaaaatc agcttatgct gacggcactt atttcgaaaa atacgtaact ggagaattcc
    13561 agccaaaatc agaaaaagtc aaagcattgt ttgaaggcat ttttatccca actgctgaag
    13621 attggaatgc tttgaaacaa gcagtaatga aagatggact gtatcatcaa aatcgcttgg
    13681 cagtagcacc aaatggctca atttcatata tcaacgatac gagtgcaagt attcatccta
    13741 tcactcgcat gatcgaagaa cgccaagaga agaaaatcgg taaaatttat tacccagcgc
    13801 cttatctatc caatgagacg attccgtatt acacttctgc ttatgatatg gacatgcgta
    13861 aagtaatcga tgtttatgca atggcacaac aacacgtcga tcaaggcatg agtttgactt
    13921 tattcatgcg ttctgaaatc ccagaaggca tttatgaatg gaagacaaca acgaagcaaa
    13981 cgacacgtga tttgaatatt ttaagacatt acgctttcca caaaggaatc aaatccattt
    14041 attacgtacg tacctttact gatgatgcag aagaaatcgg aagcaatcaa tgtgaaagct
    14101 gcgtgatcta atatcataga tagattcgct tattcatatt ttaattaaag gagcattctc
    14161 atgtcagaaa catattatga agcaataaac tggaatgaaa ttgaagatat cattgataaa
    14221 tccacttggg agaagctgac agagcaattt tggctagata cacgtatccc actttcaaat
    14281 gacctagatg attggcgtac attgtctcaa ttggaaaaag atacagtagg acatgtattt
    14341 ggcggtttga cactgctaga tactgtccaa tctgaaagtg gcatggatca gttgcgtaaa
    14401 gatgtccgca ctccccatga agaagccgtt ttgaacaata ttcaattcat ggaatccgtt
    14461 catgccaaaa gttattcttc tatttttagt acactgaata cgaaaaaaga aatcgaagaa
    14521 atttttgact ggacaaatac gaatccttat ctgcaaaaaa aagctgagcg tatcaatgaa
    14581 atctacaaac acggtacacc gctagaaaag aaaattgcca gcgtcttttt agaaacattc
    14641 ttgttctatt caggttttta tactcctctt tattatttag gaaacaacaa attggctaac
    14701 gtagcagaga tcatcaaact aatcatccga gatgaatctg tacatggaac ctacatcggt
    14761 tacaaattcc aattaggatt caacgagtta cctgaagcag aacaagaaac gttaaaagac
    14821 tggatgtaca atcttcttta tgagctttac gaaaacgaag aacgttatac agaagaatta
    14881 tacgatccaa tcggctggac agaagaagtc aagacattcc tacgctataa tgccaacaaa
    14941 gcattgatga acctagggat ggatccactc tttccagata ctgccaatga tgtcaaccca
    15001 atcgtaatga acgggatttc aacaggcaca agcaaccatg acttcttctc acaagtcgga
    15061 aacggctatt tgttaggtca tgtagaagcg atgaaagacg acgattattt gattggattg
    15121 gaataaggat ttattttaat tcaaaaattt ttcgaaaaat aaaaagagtt tagcgtacag
    15181 atattttaaa tgtacactga actctttttt atgcttattc acttgtttct tccagcactt
    15241 ttggactgtc taacttttcc gaaacatgtt ccattcttct caataaatag attttccgga
    15301 tatttaatat tatcgtctta atcactgcat agacaggtat gcaaatcagc atgcctaata
    15361 ttcctgcgac acttcctgct ccaatcaata ccaagatgat cgtcaaagga tggatattca
    15421 ttgatttccc aaataacaac ggcttgatca aatttccatc taattgttgg atcactaaaa
    15481 tcgacaacgc catatacaaa gcttgaaatg gagagataaa caaaccgaca atgattgctg
    15541 gtaccgctcc aataaaaggg ccgacatacg ggataatatt cgtgattccg caaatgaccg
    15601 caagcaacaa gctataaggc tgatcgaata ttctcattgc caaaaagctc attacaccaa
    15661 taatcaaagc atctaagatc gtactgctga tataggcaga taatgtttcg tttaattctt
    15721 ttaccgtttg gcgtaactct ttacgaatcg ctttcggaaa gaagtgagac aacgcatcaa
    15781 taaatttgtg cccatccttg aacataaata gcaaaataaa tggaacggta aataacaaaa
    15841 taaaaaattg catcataaat gaaatgattt ttgagacact tgcagataaa ctgactaata
    15901 tgatattgaa caaattacta agagataaat ttgctgatgc taattgttct tccaaattga
    15961 aatttttaaa ctcttcccgc tgcgccaatt catttaacca tttgcttgtt tcatctgcat
    16021 acattggcaa gctctgggtc aattgaattg tctgttccat taactgaggc actacattca
    16081 tgacagttaa aacaactaaa atgattacga ccaaaaacgt caataaaaaa ccgaacactc
    16141 tcgggatccg atgcttttcg agaaaaacaa cgattggatc gaacatataa tagataaaac
    16201 cagcaacaag caatggtaaa aaaactgccg aaagcatttt caatattgga ctaaaaatat
    16261 tcggcatttg taaaataaag tacacgccga tgatcgtgag tactagctcc actgtccaga
    16321 aaaataattt tgattgttta aaacgtgatt gcatacatct cctccaaggt cctttgatac
    16381 ttctattcta tcatttaaca aagagttacg cgctgagatt ttcatttgct taaaaaaaac
    16441 aaaaaaaata aacatatttt aatgagtaga cgagtactta aacaaaaaca cggtttggag
    16501 tagtgacaaa aattatactg cagtcccttt ttccaaataa acggtgttca aaagcttgca
    16561 tcttcgtaat aagcccagcc aaacgaaaaa tataaggaac tatttttatt tggctgggct
    16621 tattacttga tgtaggacat ctattttaca acctcagatc atttattgcg caatactgac
    16681 taaatgcaac tttgtgatat ttttgtgtac ggacaactcc tcgatcacat cggcataagt
    16741 gagccctttt ggaagatcaa tcgtatagat atttttgtac actcggtagt catcaacgaa
    16801 ttttacgtca aaattcacat cttcgattac gatatttttc tcttcaaaat aatcggtaag
    16861 aaaatctttg gtttcttttc tatggataaa ttggatttct aactttttag tcgtaggtac
    16921 atggatcacg cgtttcacta ctgtgagtgc aatagcaatc ccgataaaac tgatgatcgc
    16981 aatggaatag taacccatgc cgatggcgat ccctaatcca gcaattgccc ataaagaagc
    17041 ggcagtcgta agtcccgtca ccgattgttt cgtgacgatg atcgttcccg cgcctaagaa
    17101 accgacacca ctcaccactt gagcaatcag ccttgcttca tctgaacgaa tcgttccagc
    17161 aagctgtgga tactctaaag catctcttaa tgcaccacta gcgatctcaa cttggatcaa
    17221 tgcaatgata catgctccca tacaaactaa aatatgcgta cgcatacctg ctggacgatt
    17281 tttatattgt cgttcaaaac cgattgctcc gccaaatagc atagcgatcc ctaaacgtaa
    17341 aataatctct ggaattgtca ataccatatc attcatctaa acaccctcca gtccaatata
    17401 tcatgtctga aaggagcaga caaaaaaagt gcaattttta cctcttacga aaaaaggact
    17461 gtgacaaaat ttttgtcata gtccttttta tgaataaacg gtgttcaatc agctgaccct
    17521 cggtattact caacaaacgg caaaatatgg ggagctattt ttcctttgtc ttctctaata
    17581 ctcaggtcaa aacgctgatt tcacaacctc tttagcctac tattgaaaat tacaacttac
    17641 ttgctgcttc ttcatcgatg atcacaacaa cattgtcgtg attttgcaat acacttgctg
    17701 gcaaatgatt cgttacaggt ccttcaatca tccctttgat tgcatcagct ttgctttcgc
    17761 caaatgccat caagacgatt tctttacttt tcataattga accgattccc attgaaacag
    17821 cttttgttgg tacatcttct gctttatcga agaatcgttt gtttgcattg attgttgatt
    17881 ctgttaagtc aactactgaa gtcgttccgt caaatgaagc accaggttca ttgaaaccga
    17941 tatgtccatt ttgcccgatt cctaagattt ggatatcgat tggatgtgca tcgatgattt
    18001 gatcatactt tttgcatgct gcttccagat cttctgcttt tccatctggt acaaacgttt
    18061 ctttgaatgg ttttttatca aataattgga catccatgaa atgacggtag ctttgttcat
    18121 ctgaaccgcc tagtccaacg tactcatcta agtttacaga agtcatttca ctgaaatcca
    18181 aatcgcttga agtcatttct ttatataaag taactggtgt gcttcctgtt gccaatccta
    18241 atacttttgc acctttgttc ataccttctt tgatgatttc aaatgctttt tttccgcctt
    18301 cttcagcgtt ttttacacga atgatttcca tagtgattgc tcctttatat tttggtatag
    18361 cccaattata gcattctttt tttcttttta caataattag tatagaccaa attgcatttt
    18421 tttcattttc ttgacaataa ataggaatcc gtgttaggtt tcataagatg gttcaatcaa
    18481 acaatccatg ttgcatgaaa accaagcaag tgattcactg tggacgcttt atgtctgcag
    18541 tttttttatg aaaaaggagt tattatgagt atactgacat taaacaacat tacccagcaa
    18601 tttggcgaca aaatcctcta tgaaggtgtc gatctgcaat taaatgctgg cgaacatctt
    18661 gggctgattg gacaaaacgg agccggtaaa agtacattga ttaaaatcat cactggtgag
    18721 atcttaccag atgacggaca gatccattgg caaaaaaata ttcacaaagg ttacctcgat
    18781 caatatgtgg aagtcgatga aacattaaca atcgaagaat ttctaaaaac tgcgtacgca
    18841 aaagaatacg aaaaggaaga aaaaattgct tttctttacc aaacatatag cgagaccttc
    18901 gatgattcct tgctggaaaa agctgggaaa ctccaaacgg aattagacca aggagtgttt
    18961 tatcaaatcg acaccttagt tgctgagatg agcagcgggt tggggatcga tgtcttaggt
    19021 ttagattcac tgttgaaaaa tctaagtggt ggacaacgtt caaaggtgat tttagcaaaa
    19081 ctacttttag aaaacccaga tgttctgatc ttagatgaac caaccaatca cttagatgat
    19141 cagcatatcc aatggctgac gcagtttctt caggattttg acggtgcgta tatcgtgatt
    19201 tcccatgacc aagaatttct tgatcagatc accacccata tcgcagacat cgagtttgga
    19261 aagattacta agtatactgg ccatctaaaa caagcattga aacaaaagga acaaaatcgc
    19321 gaaagctatt tgcgccaata tcatgctcaa caaaaacaaa tcgaaaagac cgaagcttat
    19381 attcgaaaat acaaagccgg ttcccgatca acgatggcaa aaagccgaca aaaacaatta
    19441 gataaaatcg aacggctgac gcctccagct tcagcaagta aagctgtctt tgattttcca
    19501 ttctcgccta ttgtcacgac attagcagtg gaaacaactg atttagtcat cggatatgat
    19561 cgaccgctac ttgcacccat caatttgacc atccgctttg gtgaaaatat tgccattcga
    19621 ggcttcaacg ggattggaaa atctacttta ttaaaaacac tgatcggtga gattgaaaaa
    19681 ataagtggcg atttccactt cccagataac acaaaaatca attacttttc acaagatctg
    19741 tactgggaaa atccattaga aacaccacta caatatttaa gcagtcaatt tccaaaagcg
    19801 actatcaaag aattacgtcg ccaactagct aaagcagggt tagttaatca attagcaagt
    19861 gaacctctca ctacattaag tggtggggaa caaactaagg tgaaacttgc acagttgaca
    19921 atgaaccaag gtaatttact gattctcgat gaaccgacga accatatcga tcaagaaacg
    19981 aaagaaagct tacaagacgg gatacagaaa tttccaggaa cagtaatcat cgtctctcat
    20041 gaacaagaat tttatcaaga tcttgtgcaa cgagtcattg agattgaacc aaaataaacg
    20101 tttgattgaa tagttgaaaa aaaggagctg tgacaaaagt tttgtcacaa ctccttcttc
    20161 tgaataaatg gtggccaatt agctgacctt cggtattagc tcaaaaaacg gcaaaatatg
    20221 aggagctatt tttatttggc tattcttatt acttgatgca gaacagcttt tcacaacctc
    20281 ttttcgtcta ttttttattt tcgagatttc tctttccttt aaaaacaact gtttgaaaga
    20341 agaacgcaca tccttgaaaa acaagcccgc taaccgttac acctaaccag ccatactgct
    20401 gccacattgt cgtccctagc aatgaaccag ccgcacctcc aataaagtac ataaacataa
    20461 atacagtatt atttcggttg cttgctgctt cacctagccc ttgtactcgt gtttgattgg
    20521 cgacttgacc aaattgattc ccgacgtcta ataaaataat agctaaaagc aaaatggcca
    20581 aatggcttcc gccaaccaat aaagcaataa agcttattgt ttgcataaca agtcccatat
    20641 acacgatttt tctttcagaa taagaatctg ccagtcgtcc cacgataggt gcagcaactg
    20701 ctccagacaa accaaaaatc gctaatatcc ctgcttcgaa tgttccccaa ttgtaggcgg
    20761 gactgctgat ataaaaaatc aacgtagacc aaaagatcga aaatgttcca aacatgaaaa
    20821 atccactgac tgccgcttct cttaatagac gttgagaagc aactaaatgc ggcaaacttt
    20881 taagtgaatc aaggtatgaa acagctgtac tactttgctg gacagaacgc ggcattttcc
    20941 agtaaagcag aagtgtaaga attccgactg cacataaagc aaataaatag atcatccgcc
    21001 aagaaaaaag actggcaaca ataccagata ccgtccgaga aagcaaaatc cccacgagta
    21061 aaccgctaag caatgtaccc atgaccttcc ctcttgcttt tggtcccgct agtaccgcac
    21121 cgtaaggaat gatgatttgc ggcacgatcg aaagtaatcc tattaaaaaa gaagcgcatg
    21181 caaataacgt aaacgaaggc gcaaaaaaag cggccaacag agagacggca gataacgctg
    21241 ccattcgaat gatcaatttt ggacgatcca ccacgtcccc taaaggaact aaaaacagta
    21301 accctaacgc atagcctaat tgggtcaaca tcgtcaatat cccgactgca cttgtgctaa
    21361 cagataaact agctgctact tttgttccaa tcggctggat gtaatacata ttggcaacaa
    21421 caacgccaca agtgattgat aatagtaaaa taagcgaagt tgttaaaggt tttttttcat
    21481 tactcttcat tgatttcccc catttcctat cttagtttac aacttatgga aaagattgga
    21541 aatagaaaaa tacaactatt cgccacactt attatttgtg cttttgtttt ccttgttttt
    21601 tcttgcgatc tttataaatg aagacaacta accctactag cacagtaaca cttgcaacca
    21661 acgtgactgc agttttgtta tatccgtgaa acagagcttc tccaccacac gcatacaaca
    21721 cagacgaagg gatgactcca atcacgatcg acaatatgat ttgctgtaga ggcaatttca
    21781 tcgcatctgc agcaaaattg accatagaag atggaataac cggtatcata tagcctaaca
    21841 ttacacctat ttttggatgt ttcatttgtc ggattgcttg cacccaatga ttcgtttcgg
    21901 ttttcttatc taaaaatttt aagcgatgca taaggaaaat tgcagtgatg ttaccaagag
    21961 cattacccaa aatgttgatg atcgcacctg tcagtggtcc aaagctcaaa ccagcaatga
    22021 ctccaactac tgaagtagga atacctggaa cagcacacat caccgctgtc aatacgatca
    22081 agagtatcga tgcaaaagtc ccatgcgaac gaatatgtgc gagtagctct gccttactcg
    22141 ctctcggatt taaaaataat tggatgtctg tctgatattc taaatacaac cgatagccca
    22201 ctaccagtat cagaatgatt cccaacgaga tcaatatcaa tcggattatt ttctttttca
    22261 tatttcctcc tgaaaaaaat ctatattcat taaataatag tttaacataa aaacaaaaaa
    22321 gcaccgaaat ccaacatagg atctcagtgc ttttaaatag taaaaattat ttaacagtta
    22381 cagatgcgcc aacttcttca agtttagctt tcaattcttc agcttcttct ttagatacgc
    22441 cttctttaac tggtgcagga gcgccatcaa ctacagcttt agcttctttc aagcctaaac
    22501 cagttgcttc acgaactgct ttgataactt taactttttg gtctccagct gcagttaatt
    22561 ctacagtgaa ttctgtttgt tcttcagcag cagcagcgcc accagcagca gctacagcta
    22621 caggagcagc agcagatacg ccaaattctt cttcaatagc tttaactagg tcgttaagtt
    22681 ctaggatagt tgcttctttt aactcagcaa cgatgttttc aatgttcaat gccattataa
    22741 tttcctccgt ttaattgttc ttttttattt aatgctttat gcggcaatta agctgcatct
    22801 ccatcttctt tgtctgccac tgctttgaca gcgtaagcca cgttgcggac tggcgcttgt
    22861 agtacagata gcaacataga tagaagtcct tcgcgatttg gcaattttgc caatgctgtg
    22921 atttcttcca atgaagagac tttaccttcg atgataccgc ctttgatttc taatgctttt
    22981 gcttctttag caaattcgtc catgattttc gctggtgcga ctacatcttc gttactgaat
    23041 gcaacagctg taggtccagt gaatacttcg tccatgcctt caaggccagc ttttttagct
    23101 gcacgtgaaa gaatagaatt tttgataact ttcatttcta cgttagcatc acgaagttgt
    23161 ttacgtaaac gtgttacttc atcaactgtt aatccgcggt agtcaacgac tactactgat
    23221 gcagctgctg taaatttttc gcttacttca tcaacgattg ctgctttttt tgcgataatt
    23281 gcttcactca tttttgagtt tcacctcctg aattttgatg gaagagtttt ttgcactgaa
    23341 aaggcaagtc acttttatag ttactttgct caaccagtat tattcatcac ctattcactt
    23401 cgttcatagt gattctactc gctttgctcg aaaggaaact cctttcgctt tgctcaacca
    23461 gtattgttca tcatctcttc acttcgttca tagtgattca caataaaaaa ctctatgcca
    23521 ccgcagacat agagggacta ttgaatatat tcaataaacg tcctcggtag gaaattaagg
    23581 cttgatgcca cctactgtct tcggtacaag ttaattatta acctcaacta gcttagcatg
    23641 ctaaactagt tgagtcaaga attatttttc agttggatca atcaacattc taatatttaa
    23701 ttagaaagaa gcttggtcaa catggatacc aggtccaaat gttgttgtga tcgttacatt
    23761 tttcatgtat tgacctttag ctgctgatgg tttagctttt agcaatacat catggattgc
    23821 tgcaaagttt tcaactaatt tttcatcgtc aaatgaaact tttccgattg gtacatggat
    23881 atttccagct ttgtccacac ggtaagttac tttaccagct ttcacttcgt ttactgcttt
    23941 tgttacgtcc attgtaactg taccagtttt agggtttggc attaagcctt ttggtcctaa
    24001 tactcgtcct agacgaccta cagtagccat catatcaggt gttgcaacaa ctacatcaaa
    24061 gtcaaaccat ccgttttgga ttttttgaac catgtcgtcg tcacctacga aatctgcacc
    24121 agctgcttca gcttcttttg ctttgtctcc tttagcaaaa actaaaacag tttgtgtttt
    24181 accagttccg tttggtaata caacagcacc acggatttgt tggtctgctt ttttagggtc
    24241 tacgttcaaa cggtatgcaa cttcaactgt tgcatcaaat tttgcaatgt ttgtttcttt
    24301 tgctaaagct actgcttcgg caacaggata aactttactt gaatcaactt tttttaatgc
    24361 atcttgcatt tttttgctct ttttagccat tttggttcct ccttgattgt ggttataacg
    24421 gttgtacctc ccacgtgttc tatatctttc aatatagagc caactgagaa gctgctgtct
    24481 tagggtgtat agattattct acagtgatcc ccatgcttcg tgcagttcct tcaaccatgc
    24541 gcattgctgc ttcaacgtta gctgcgttta ggtcttccat ttttaattca gcgatttctc
    24601 ttacttgatc gctagaaact ttcgcaactt tatttttgtt tggttcgcct gaaccttttt
    24661 cgattttagc tgcttttttc aatagaacag cagctggcgg tgtttttgta acgaatgtga
    24721 atgaacggtc ttcgtataca gaaataacaa ccggaatgat caaacctgct tgatcagctg
    24781 tacgagcgtt gaattctttt gtgaatccca tgatattgat acccgcttga cctagtgcag
    24841 gacctactgg tggagctgga gttgctttac ctgcaggaat ttgcaattta acgatttttt
    24901 ctactttctt tgccacgaga catacctcct taagtccgtg atgtggttaa ttggagatgc
    24961 atatttctcc tcccacgttt tatctgatct gaaaaaatcc agacaaataa tgtgcaaaac
    25021 aatgcacact ggtacagtat agcaaattta tttgtttttt caagattttt ttctgctatt
    25081 tttacatttt gcttgaataa acgctaaatt tattctaaaa aaacaacaat ttttcgttct
    25141 catttcttct tttatgttac acttgcgcta gcatggttca acagatgaaa gcaaacaata
    25201 acacggtttt ttagaccctt gtacaaagat tcctacataa caaaatgagg tggagaaaat
    25261 gaaagattta aggtatagaa gtgtctttga tattattgga cctgtgatga ttggtccaag
    25321 tagctctcac actgccggag ctgctagaat cgggaaaatc atacgtagta tatttggcga
    25381 acagcctgat tctgtagata tttatctata tgaatcattt gcaaaaacct atcgggggca
    25441 cggaacagat attgcattag ttggcggcct gttagggatg gaacccgatg atgaacgttt
    25501 agctgattct ttgaaattgg catatgaagc cggtatggaa gtcttgtttg ttcctaagag
    25561 tgaaaaagcc gaacatccaa actctgtaaa gatgcttcta aaaaaaggaa cacgaaagct
    25621 ttcagtcact ggcatttcga tcggcggtgg caatatccag atttctgaat tgaatggatt
    25681 caaaatttct ttaagcatgg gtactcctac cttgatcgtt gtccaccaag atgttccagg
    25741 tatgattgct aaagtgacta gtatactttc tgagaccaat acgaatatcg gcactatgac
    25801 agtgacacgg gaatcaaaag gtgaaaatgc cattatgata attgaagtag atgatcctca
    25861 agtagaagaa acggtaaaaa gactcaaaca gctgccaaat atcgacagcg taaattattt
    25921 cgattaagga gctaaaaaaa tgtttttatc gattgaagaa ttagtagatc aagcaaaagc
    25981 ttatccaagt gtagcagaat tgatgattgc agttgaaatg gaaatgagtg gccgttcccg
    26041 tagtcacatc atcgagatca tggaaaaaaa cctctcagta atggaacaat caatcaacga
    26101 gggaaaagaa ggtgttacct ccgtcacagg aattaccggt ggtgatgcaa aaaaattaga
    26161 tgattattta aatgaggcag actttttaag tggagaaaca atcttaaaag ctgtacgcaa
    26221 tgctgtagct gttaatgaag tcaatgcaaa aatggggttg atttgtgcta caccgactgc
    26281 cggaagcgcg ggtgttgttc ctggtgtgct gatggctgtc cgagatcggc ttgatttgac
    26341 acatgaacag caattggaat tcttatttac cgctggcgct ttcgggttag tcatcgcaaa
    26401 caacgcttct atcagtggtg cagaaggggg atgtcaagca gaagttggtt ctgcaagtgc
    26461 aatggcagca gcagctttgg tttgtgcaaa aaatgggact cctcatcaag cagcacaagc
    26521 agtagccatt acattgaaaa atatgatggg actgatttgc gatcccgttg caggcttagt
    26581 tgaagtcccg tgtgtcaaac gtaatgcttt aggctcttct caagctttta tctctgctga
    26641 tatggcatta gcgggtatcg aaagtgtcat tccgccggat gaagtcgttg ctgcaatgta
    26701 ccaagtcgga cgtcaaatgc cgtcgatttt caaagaaaca gcagaaggtg gcttagctgt
    26761 tacgccaact gctaaacgac ttgcggaaga gattttaaat aagagggcgt gataaagctc
    26821 cttaatttcg agtattaaga gattagttat cttcataaca aaaaacgaag agaggactaa
    26881 ttgcccttct cttcgttttt tattaatcca tcaatttcgt attttttact tcatcgatcg
    26941 tttttgttcc tgcaagttgc atcgtgatcg acaattcttt gttcaggtgt tcaaaaacag
    27001 atgttactcc ttctgcccca cctagattca acccataaat cactggtcgg ccaatcgcaa
    27061 ctaagtcagc accacttgcc agagctttga agacatgctc tcctcgacgg acaccagagt
    27121 cgaaaatcac tggtacacgt ttgttgacta cttcggcaat ttttggcaat acttcaaagg
    27181 acgcaggacc tccatctagt tgacgtccac catgattgga tacccagata ccatctgccc
    27241 ctgcagagat agcgattact gcatcttctg gcgattgtat tccttttacg ataactggaa
    27301 gatgggtgat ttctttgatt gttttgatgt catctggtgt caagccttgt tttgcagctg
    27361 cataaatttc tgcgattcct tttccttcgc cattaccact tgcactttgt tcgctatacg
    27421 cagctaagtt aggcattggt aatggaaatt ggaattgatt gatgacgtct tcttcacggt
    27481 aaccaccgag cgttgaatct gccgttaaaa tgatagcttt agcccctgct tctactgctt
    27541 tgttcaaaat gaactcattg aatctgtcgt ctttactcat atataattgg aaaaactgcg
    27601 gtgcgcctgg cgctgcatcg gctgcgtctt tgattgtcgt atttgcataa gtgctgatgg
    27661 aaaagattga tccagcagca gctactcctt tagctgtatc agcttcccct ttttcatgtg
    27721 ccaatccttg ggccgctgat ggcgcttgga taatcggtgt atctaattca attccgaaaa
    27781 cagatgtatg caaatcggct gagtcgattc cgcgtaatat ccgtggcatg attttctttg
    27841 tattaaaaga agtcgtattt tctttcatcg tccactcatc ttcagaacca ccgcgaatat
    27901 agccaaaagc tcctgcttcc atgcgctctt ttacacgacc ttctagttag gcaatgttta
    27961 caatatcgat tggatgttct tcattacttg tatgataagt catagtagat ccttctttca
    28021 tcttcttgat tacatataaa tcatactaaa cttacaaaaa gtaagcaaac atttactctc
    28081 agatactttt atcgctattc tttccaaata aatcgactta caagctgatc tacttcttgg
    28141 ttttcatgaa gcatactatg agaggcattt tcacctgtca ctactttttc ctctatttca
    28201 tttccgcgtt gcttcaacaa cgcatagacc gctaatgcac tgtttaacgg tactgttcca
    28261 tcactcaagt ctgtttcatc tagttgacca gcaatcaaga aaaatcgagt ggttatagga
    28321 atattaccga tcatttgctg gtaatcttga tagcggttag aaacgacagc aggccccttt
    28381 gctaactcat ctgtcagaga ctgcgcacta tcatcgacaa aatcattaaa aggcgcgcca
    28441 attgctgtta atgtctgaac ttgtggcaat gacgtatcct gtccatacgt tcctaaatag
    28501 cgtagtgcac taactcctcc catggaatgc cccactatgt tgactttatc tatgccgtag
    28561 ttttctttca gatacaaaag acaagttttg atccattctg cttgattcca ctcgttattt
    28621 ttattatctt caaacaatac ttggaccatc ggattgtctg cttttccaga caaatctcct
    28681 gttgcttgta tttgtccatc tgttcctact gttaaaacca attcttttac tgcttgctga
    28741 tttttttcca aacgccgcaa catcccttta aaggaatttt ctgtcccgct gtagccgtgg
    28801 aaaaaaagtg ttggaatagt ggttttttct atcactttac tggattctgc cgttttttct
    28861 ttatcagaaa cttcagtagt atttatcttc gtcgtctggc aaccacttaa cgcaaacaaa
    28921 ctgataagga ctaaagctgc tgcagttgca cgtttgcata aatttttgat tggttctgtc
    28981 atacacctct tcctttccat atagaaaaaa gaaagacttg aaaggccaat catatgatcg
    29041 acatttcaag tctctttatt aatcgatttt atctacttga tcgaaatcaa gttctgtact
    29101 ggtttcacgt ccaaacatat cgatgttgac tttcaatttt tctttttctc cgtcaacttc
    29161 tgttacttgt ccttctaatc cagcaaatgc gccttcgatg attttcacga cttcgccaac
    29221 ttctacttct agatcatttt gtcgcaggct cataccgata gaacgcaaga tatgattgac
    29281 ttcttctgga agtaatggtg ctggtttgct tccggctccg tgtgaaccaa cgaatcctgt
    29341 cacaccaggt gtgttgcgga caacatacca tgaagcgtct gtcatgatca tttcaacaag
    29401 tacgtatcct gggaatgttt tatgaacgat ttctttttct ttcccatttt tgacttcagt
    29461 ttctttttct tcaggaacaa cgacacggaa aataaagtcg gccattttca tactttgcgc
    29521 acgagattcg atatttgctt ttactttgtt ttcatatcct gaatatgtat gcaagacata
    29581 ccagtttttt tcaaaagatt ccattgaagg ctcctcattt cattgtaaaa taaaaaaacc
    29641 tcagtacacc cgaagttttt ctttcgcttc attatagcac taaaagtacg ttgtggctag
    29701 cgagttattt taaaatccag ccaaataagc tttggatggc tgtatccatt acgtaaaaca
    29761 tcaatccgaa aataatagaa gtttcgatga ctactaacgt atctttacgg agttgttttc
    29821 tgcttggcca agttacttgt ttgatttcat cttttacgct ttttagaaat ttcatgagac
    29881 aacctccctt gctactgttt atttcgtttc tttatgtaat gtatattgat tacaatattt
    29941 acaaaactta ttgatttcca aacgttcgcc gctttttcct tcactgactg attttgtgta
    30001 gttgcgagac ccacaaacgg aacaagcgag tgctgctttt ttctttgcca tttacttctg
    30061 cccccttgct gagaatcaga cttatacttc ataacagtat cacgtaactt tttacttgtc
    30121 aatttgtgaa ataaactatc tattgttttg gataaaatta tgatatgatt aatttgaaat
    30181 taagctaaaa tgagacaatt ggaggtatag cgatgctatt aaaaacactt gtatataaga
    30241 aaaaagatct gacaaccgtt acagagacta gtacattaga agaagcatta aaaattttag
    30301 aaacttccgg ataccgttgt gtgccgatct tagattcttc aggtaatatt ttccgtggaa
    30361 acatttacaa aatgcatatc tatcgtcata aagcaaatgg cggtgatatg acgcttcctg
    30421 tcactcatct tttgaaaaac gctacaaagt tcatttacct ggatacttct ttcttcaaag
    30481 tattctttac gatcaaagag cttccttata ttgctgtcct tgacagtgaa aatcattttt
    30541 atggtatttt gacacatagt acgttgctta atatgctctc tcaatcttgg agcgtagaac
    30601 aaggaagcta tgtactgaca attgcttcca caggaaagca aggagatttg gctgctattg
    30661 caaaaatcat ttcaaaacac agcagcattg ccagctgtat tactcttgat atcgaaaaag
    30721 acgaatacat tcgacgtaca ttgatcacgc taccggctaa cactgaagcg aaagtatgtg
    30781 aaacgatcgt caaccactta gagaaaaaga attataaagt ggtagaaatt gaaaatttgc
    30841 aaaaccaaga agcataatca aaaagcgaaa aaagaaaaca gctaatctgc tttctttttt
    30901 cgctttttat aattagacac gcttttcaaa atcaagcgcg cgatctaaat aatacatcaa
    30961 tggtgctgaa acagccatga cgataaactc acttaatgca agcgtgccat aggttgtcca
    31021 aaatgcttgg ccacctgttg gaactaacat ataagcaatc agtcccatgc tgatggtaaa
    31081 gaagatcgtg ttcaatacta aacgcatctt tacatttttc actttgtttt gcaataaagc
    31141 tgtcaggcct aaagaaagca atgtttggcc gccaccaaaa accacatcca tgataccaaa
    31201 accaaagaag gcattataga cgatgacccc gcctaaaact ccccaaagca atttacggtt
    31261 aaagacaact aaatgattca aactttctga aatacgaaac tggatcggcc ctgaggatac
    31321 aggcgcaact aaaatagtta acgctagata aagagccatg atgatgccat ttacaacgat
    31381 tacggataac cgttgtctgc ttttcgtttt ttgcattttc tctcctcttt tctcctagtt
    31441 tttttaacgc gggatggttg atgaaccgcg gttatgtgaa acaaacaata attataacag
    31501 acttttcatt tatttcaatc cgtcttatct gcttttaaaa aagcaaaagc ttaaacaatg
    31561 taaacgtttg ctaaaaatcg tgggatatgg tataaagtaa gtgtaagggg tcataagaat
    31621 tcccagtaaa gcagacgaag taggatctgc aagaacagaa ttgttgtgaa acttaaataa
    31681 ataaaaaagg atgtgggaat ttgagcaacc tcttactaga gaacggtcta gcaagcagct
    31741 gaagatctaa gtggacatgc cgaaccttgg aacttctcct tacgtaagta atggatcatg
    31801 cgcttgctag gcttttttgt gtttttcggt cttcggacct ttttcatttt cctagacttc
    31861 tgctataata accaagaatt aattttgtaa cagatttttt tgaaaaaaca agtttctttc
    31921 attcaaaaaa tagaaaaagc ttgctaatta ttggttaacc agcaaacagg agtattaaaa
    31981 tatcttgttt tattgaatag caggaggaaa tcatgaaatt ccattatcat atattgatca
    32041 atccatcggc aggcagcgga aatggctata aagtagcaga aagaatttta ccggtattaa
    32101 aaaataagca tattgactat acacttcatt atagtgaata caaaggacac gatgctgaga
    32161 ttgctgaaac tttggcaaaa gaaacattga ttccttggat agaagaagaa cagaaaacaa
    32221 tcgatacttt cccccttttg atcatcgtag gaggtgacgg caccttacat caggtacttg
    32281 atactttcta tcagatggaa gtagaattcc cagttgctta tattccagca ggttctggag
    32341 atgattttgc tcgaggggca ggccttccaa aaaatcccaa aaaaggactt cagctgattt
    32401 tagcagctca atcaccagaa aaagtccaca taatggctta tgaagaaaaa ataagtgaaa
    32461 aaaaaggaat agctgttaat aatttcggta taggattaga tgcagctatc gtccatgcga
    32521 caaatcattc ttccacaaaa aagcgattga acaaatacaa cttaggatca ttttcgtatt
    32581 tattctctat tctccgtgct ttattcactc aaaaaggatt tccaattctg gtagatgcag
    32641 gaggaaaaca attaagtttt tcaaacgcat ttttatgtac agcaacgaag catccttttt
    32701 ttggcggagg aatcgcgatt gttccaactg ctgatgcgtc taaaccagtc attgattttg
    32761 tcgtggtcga acgaatcaat ttctttaaaa tcttgtggct cattctttta cttatacaga
    32821 aaaaacaatt gaaatcgaaa tatttccatc actacacaac aaacagatta cgtatcgttt
    32881 caacaacacc gcaacatggt caagaagatg gagaagaaat ggaaaaacga ccttttgata
    32941 tcacaataac cacaactaaa cagttgtttt ggtgttaata aaagaggttt tgtcagaagc
    33001 tacatctgga acaccgttta ttcggaaaaa aggactgtga cacgactttt gtcacagtcc
    33061 tttttttact atgcagttaa ttctttgccc aattgggttg ccatcgatag tttctttctt
    33121 tacccaaaat aaattggatc attgaaaaca ccgcagcaaa agcaactaag acacaaccat
    33181 tgaaccaaaa agattctgca aatgtttgat tgtaactgag tgtttcactg atcaagttat
    33241 tattttgaac aaaccaatat gttggtgtga atgccgctat tttgctaacc atttctggca
    33301 agtattctgc tggtacaaac accccaccga taaagcaact gcctgtgata aaaatatctt
    33361 tgatcccacc gctggtttct gagtttttta ccaatgaagt gatacaagcc gaaaagctaa
    33421 tgatcggcaa taaaaacaag aaagtattta aaatatgata ccataccgtt atatcccacg
    33481 aggtgtggat cactaacaga accattagaa gtaagaagcc actgaacaac agcagcgagt
    33541 aactaatcaa actaaaggtt actttgcgat tcaagtttcg tttggtaatg ggcgcactct
    33601 ggtttcgttt actgatctct ttgcgattaa atgctagatt gatgacgctg aaaccgctaa
    33661 aaatggatag aaacaaccca taagacaaga aattaaaggt tgtgcccgtc aacagctttt
    33721 tcatccgctg actatatccc gcacttagct caacttctcc agtaaccgag agtgccattt
    33781 gtgtctgttc catagctgca tcgattgttt tatcttcttc ttgaaacaga atcagtgttt
    33841 gcaaaaaggt gttcacttgc tgtgtaacaa ctgttttaga aaaggcatct gggcgactct
    33901 gtatcttgat cgtcggcatc tttcctactt cgacttgttc ggtaaagtct gcaggcagat
    33961 gtaagataat gtttacacga ttaaaataaa gagcatcatc gatttctttt tgtgaggttc
    34021 catccaatgt aacgactgtt tccttgtcat tcaaatattt acgaaggcca tcgactacag
    34081 gcgactcttc ttcactaatc aaggcgattt tcgcatcttg cagagaggta ttttcaacat
    34141 caatattttg cgcaaaaaag aatgtggcaa caacagtgat aattacacct atcagtaaat
    34201 tcgatttatt tgctttaagc aatgtccaaa tcgttttata aagcgtcata acgcacccct
    34261 ctttccatcc aagcatttcc taacgcaaaa aggaaaataa atccgatcaa ccatagaaga
    34321 ttggtataaa aactagccaa atcagtgtag aaataaagtt t
//
LOCUS       DARBET010000025        33085 bp    DNA     linear   BCT 07-NOV-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4027
            SAMEA114569841-rid20112183.denovo.025, whole genome shotgun
            sequence.
ACCESSION   DARBET010000025 DARBET010000000
VERSION     DARBET010000025.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA114569841
            Sequence Read Archive: ERR12203666
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 33085)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 33085)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001972618.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001972618.1
            Genome Coverage       :: 132x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/07/2023 13:18:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,041
            CDSs (total)                      :: 2,975
            Genes (coding)                    :: 2,908
            CDSs (with protein)               :: 2,908
            Genes (RNA)                       :: 66
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 67
            CDSs (without protein)            :: 67
            Pseudo Genes (ambiguous residues) :: 0 of 67
            Pseudo Genes (frameshifted)       :: 26 of 67
            Pseudo Genes (incomplete)         :: 28 of 67
            Pseudo Genes (internal stop)      :: 20 of 67
            Pseudo Genes (multiple problems)  :: 6 of 67
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..33085
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA114569841-rid20112183.denovo.025"
                     /isolate="VRE4027"
                     /db_xref="taxon:1352"
                     /country="Denmark"
     gene            203..1078
                     /locus_tag="SCJ86_001298"
     CDS             203..1078
                     /locus_tag="SCJ86_001298"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302697.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEG1168872.1"
                     /translation="MNNWLEYFPENVLERGYSYHLHGFVRHLNYTSKYLSATVSGTED
                     YKVVITWDEKTNMTCDCLYAIEGKKCKHMAAVLFAYEERPIKKSNYSLSELSSLVSSA
                     SSSLVRELLTEILIEHPQFIERFKVKMPFHAINYSDKLTTIIHKYDHIIKKNKNRKTA
                     KFIMEMRKFIQEAVESLIQQNAYLPAFELINEVIATLETFYWEPEDERTLLLIEDCYY
                     LWKELLAEAPHAEKRQMFSWFVCQVDHTDASYSKRYSIKILKEDFREKEFSNQKKKID
                     KKTKETVKKDDFNEK"
     gene            complement(1223..1375)
                     /locus_tag="SCJ86_001299"
                     /pseudo
     CDS             complement(1223..1375)
                     /locus_tag="SCJ86_001299"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005874852.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="xylulose kinase"
     gene            complement(1643..2974)
                     /locus_tag="SCJ86_001300"
     CDS             complement(1643..2974)
                     /locus_tag="SCJ86_001300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318823.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HEG1168873.1"
                     /translation="MGKSSRKDIVFEKFGIVATKLGNQIHLRTLRDAFATFMPFMMLA
                     GFVTLINSVILEPTGFMGEIVDTSTLTTLQQIGTSIANGTLSITTLLVVAAVSYHMCA
                     SRNYTNHIAAVLVAISTFVVLTPMQMMFTPENADKAVEVTGVIPISYTGASGMFVGIF
                     VGLAATELFIKLSNNKKLQIQLSGNIPPAVLKSFNVLIPIIITITSFALLSFIIIQLF
                     NMDINKLITTIVTEPLSKITTGLPGFLLITSIANLFFGFGIHQAVISGSLLDPFLIQN
                     MQENMAAYANKEQIPHIINMAFKDTFAVMGGSGNTIALLIAIFIFSRRKDYKDFAKLS
                     VTPAIFNISEPIIFGLPIVFNISLIIPFVLAPIFSLTVAYFATAVGLINHVVVQIPWT
                     TPPVISGFLATAGDWRAAVLQILIIAASVFIYLPFLRIDEHVTTKLSENET"
     gene            complement(3122..3835)
                     /locus_tag="SCJ86_001301"
     CDS             complement(3122..3835)
                     /locus_tag="SCJ86_001301"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016611336.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HEG1168874.1"
                     /translation="MKKIAKYMEIYLDIKKKIVNGEYKIGEKLPTGDELAVIYHTSKL
                     TVKKGVDLLVSEGVLRSRSGYGTEVVRTPIDNSRVLGPNEGLFSVVGEEHVDSEIHTF
                     SIELPSKKVADKLSISVKDYVYNIVRSRFIDCQPYSIEQTFMPLAVIPGLEPKHLKKS
                     VYAYITNELGLEIKTSHIRIKGDTANEFDAKILKIDPGEFMIEIDKVVALSSGKPFEY
                     SVSRHLYQDFVFEAVFVEK"
     gene            4010..5434
                     /locus_tag="SCJ86_001302"
     CDS             4010..5434
                     /locus_tag="SCJ86_001302"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339702.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 1 protein"
                     /protein_id="HEG1168875.1"
                     /translation="MTTMKKLTFPDDFWWGSAWSAEQAEGKGETGKAETIWDRWFKEQ
                     PYRFYQRIDSSITTDHIHQYKEDIELMKKTGHNSFRVSISWARMFPNGGTGKVNPKAI
                     SFYRKLFSEMNNNGIKVFANLYHFDMPATLQDKGGWESREVVDAYVHYAETCFKEFGD
                     LVCHWFTFNEPLGPILGTYLEDFHYPNLIDFKRGAQAAFFTILAHAKAIQVFKELALP
                     SKIGVILNLSPTFPRSQNPADVKAAEIADLFYTRSFLDPMVKGNFSKKLILLLQEYNQ
                     LPEDYTKEDLVCIYENTAQILGLNYYEPRRVKSRSSMVDTSSPFLPEWFFEPHIMPGR
                     RMNEYRGWEIYEKGIYDLCMDIKNHYGNIEAFISENGMGVADEERFMDDTGQIIDDYR
                     IEYIKDHLAYLWKAINEGCNIKGYHLWTFIDCWSWINAYKNRYGLVSLDLTTQKRTIK
                     KSGEFYKQLSDTNGFRYDVDLLVE"
     gene            5733..6440
                     /locus_tag="SCJ86_001303"
     CDS             5733..6440
                     /locus_tag="SCJ86_001303"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AzlC family ABC transporter permease"
                     /protein_id="HEG1168876.1"
                     /translation="MDEKLDIKTAIKDTLPTVFGYIGIGLAFGIVGKAAGFHPLVVTL
                     MSLLIYAGSAQFITVSMLASHSPLLSIVLSTFLVNSRMILMSMTIAPYFKKNRLLQNL
                     LIGTLLTDESFALGMNKLNYTGQKLNFRWMNTANWISYLTWVGSSLVGALLGNFITDP
                     KKFGLDFAIVAMFIGLLYLQVISDRNTSKRLQLILIGLTLILVYVGLIFIPSNLVILV
                     VTLIGCGLGVWIKHAFF"
     gene            6427..6750
                     /locus_tag="SCJ86_001304"
     CDS             6427..6750
                     /locus_tag="SCJ86_001304"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287484.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AzlD domain-containing protein"
                     /protein_id="HEG1168877.1"
                     /translation="MPSFEFTLLTILGCTLATWLSRILPFVLLKKFDLPQPLLEYLSF
                     VPIVIMSALWFSSLFTQNIGHLPQINLENALASVPTLLAAILSKSLLVIVLVGILSLS
                     LIRLL"
     gene            complement(6839..7876)
                     /locus_tag="SCJ86_001305"
     CDS             complement(6839..7876)
                     /locus_tag="SCJ86_001305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329811.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-binding alcohol dehydrogenase family
                     protein"
                     /protein_id="HEG1168878.1"
                     /translation="MSEKELMKVVGFYEGLSIDDPKSFIDEKEQIPVPSSRDLLVKVN
                     AVSVNPVDTKLRQDNGIRNALRILGFDGVGKVVAVGDEVQKFSVGDRVFYAGTTTRAG
                     SNQEYQLVDERIVALAPKNLSDEEAAALPLTSLTAYELLFEKFGLTPEENANRGKKIL
                     VINGAGGVGSILNQLAHWAGLEVYATASPKNFEWLKKTGVDYPIDYHHDLSKSFRELG
                     IDQVEYVAVLFDITRYFDQIKHLVRPFGHVGTIVGIKEPLDIGVWKNQSISFDWEYMF
                     AKTDYNYEIETQGEALAHIAALANEGIIRSTLSKVYSDGMNAKNLKQATKDVETGHIL
                     GKVVVSGPFNG"
     gene            complement(8197..9180)
                     /locus_tag="SCJ86_001306"
     CDS             complement(8197..9180)
                     /locus_tag="SCJ86_001306"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008268478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA-dihydrouridine synthase"
                     /protein_id="HEG1168879.1"
                     /translation="MTTNFWKELPKPFFVLAPMEDVTDVVFRHVVKHAAAPDVFFTEF
                     TNSDSFCHPDGKDSVRGRLTFTEDEQPMVAHIWGDKPEFFREMSIAMAEMGFQGIDIN
                     MGCPVPNVAERGKGSGLILRPEVAAQLIEAAKAGGLPVSVKTRIGYTEMSEMEEWISH
                     LLHQDIANLSVHLRTRKEMSKVDAHWEVIPKIIALRDQIAPQTLITINGDIPDRQTGE
                     ELAEKYGVDGIMIGRGIFKNPYAFEKEPREHTEKELIGLLRLQLDLQDHYSEIIPRSI
                     TGLHRFFKIYIKGFPGANDLRVQLMNTKSTDEVREILDTFEKEHPTLFSEQ"
     gene            complement(9595..10365)
                     /locus_tag="SCJ86_001307"
     CDS             complement(9595..10365)
                     /locus_tag="SCJ86_001307"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002354811.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEG1168880.1"
                     /translation="MFNLYFTALKSLAIKETNRYLRIWVQTLVPPVITTSLYFVIFGK
                     MIGGRIGDMGGFSYMEFIVPGLIMMSAITSSYANVSSSFFSQKFQKNIEEILVAPVPT
                     HVIIWGFVIGGVGRSILVGSLVTIISLFFVPLHVYSWFMVIITLLMTAILFSLAGLLN
                     GIFAQSYDDVSIVPTFVLQPLTYLGGVFYAISMLPPFWQAVSKVNPIVYMISGFRYGF
                     LGTIDVPVATSMIVLVLFIVVLYAVTWYLINKGRGLRS"
     gene            complement(10358..11293)
                     /locus_tag="SCJ86_001308"
     CDS             complement(10358..11293)
                     /locus_tag="SCJ86_001308"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376359.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEG1168881.1"
                     /translation="MANALEINNLKKVYGSGVEALRGIDLSVKEGDFYALLGPNGAGK
                     STTIGIITSLVNKTSGKVNVFGYDLDTDLVRAKQQIGLVPQEFNFNPFETVQQIVVNQ
                     AGYYGVSRKEAMKRSEKYLKQSNLWEKRNVRARMLSGGMKRRLMIARALMHEPRLLIL
                     DEPTAGVDIELRREMWEFLRELNENGTTIILTTHYLEEAEMLCRNIGIIQSGELIENT
                     SMKELLSKLQFETFIFDLAPYETKPVIEGYACTFEDEQTIAVEVERNQGVNGIFDQLT
                     RQGIKVLSMRNKSNRLEELFLKITEENNHTEEKNV"
     gene            complement(11609..13258)
                     /locus_tag="SCJ86_001309"
     CDS             complement(11609..13258)
                     /locus_tag="SCJ86_001309"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307759.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA mismatch repair protein MutS"
                     /protein_id="HEG1168882.1"
                     /translation="MGQREFIVLIIIAAVILLVALDIFSRLKLKETVRSKWEKIPYQP
                     RFDKEESLKEAWLTEKKFRSWDSEIDDLTWYDLDMFEVFEGINSTYSSVGSEALYQRL
                     RSFDFGEDQQLEKLIAFYQENPQLREKIQYQFARLGKKDHNFAKQYLADGKSKKIGRV
                     GLFIFLGLLPLVGLGLLLLGQVLGIYLAIGGLVFNTVYYLVKKQTLETELNSMSYLVS
                     TISAANQLAKLPTPLQEELKQQTKPLRDIPKLGFSFRMKSGSEAELIFEYVNIMFMLP
                     FISYHFVLTKLEKHSTEAIKLWELLGELEVAAAILNYRTYMPITCRPEFTEGGVTAKD
                     IYHPLLKEAVVNPVNWQKNTLVTGSNASGKSTYVKSIAISCILAQTIQTAIAEQFTLQ
                     PGHVLTSMAIEDDLFEGDSYFVSEIKSIKRLLDQVASKERCYCFVDEILKGTNTIERI
                     ASSASVVRWLAEYPSLAFVATHDIELTEILKNYCENVHFEEQVTEGKGISFDFKLKEG
                     PSRTRNAIALLKVLDYPETLVETAKTEAALFDEKRQWHVLG"
     gene            complement(13375..15978)
                     /locus_tag="SCJ86_001310"
     CDS             complement(13375..15978)
                     /locus_tag="SCJ86_001310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344815.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-mannosidase"
                     /protein_id="HEG1168883.1"
                     /translation="MVNAHIIQHTHWDREWYFTNEDAIVLSDQVFTEVLDELERNPKV
                     NFCLDGQTSIVDEYLEINPQKLKVIQRLMKTGQLFVGPWYAQTDALLVDAESILRNLM
                     IGISDTILKYGDPMMVGYLPDTFGFNANLPTLLHQVEIDNFMCWRGLNFETMVPSPYF
                     IWKGLGDKFVYAMNFPFGYMTGMMTLDAIKDIPEFAKEKLDEAVEMLHMQGKNKDILV
                     PSGVDQKSMILNFDQVVEKLNKYSKFHNIISDYPSYVEVIRNKKELPEYKGELRQPVY
                     SRVHRSIGSVRTKMKIENFKLEQKILKRVEPLMLIAERNGIHISKGLLRRLWKKILQN
                     QAHDSIGGCVSDNVAEDIFHRIKEANEIADGIENLIVKRMADALKLKSNEVLVFNTTT
                     ENYSGSKIVHVVADSKKIHFKGSPYNIIEKEVYFPERKNAYRMIATGFEYFDEPAYYE
                     LDIKINVNVPALGYTVVEFEKNNEKLETAIEIDKTEISNNKYKLSFKDGQLNLIVGDR
                     QYLDFVHLIDSANDGDTYDYSPLEGDTELSLKLETAKVYKDSLQETLVVYGKAQLPKD
                     LKDRLSEKPEMEEISYEISFSLGESQIVEGTLKIHNTVYSHRMRLAINVLDKSEKSIA
                     QIQNGFIENTPDVIPPNWSETMVEKPVNLEIFDKSVSVAENDHYFTFYADGLKEYERI
                     ENQLIITLFSTTGELGKPNLAWRPGRASGDTTNEGHVMMETPLAQEIGEYKVTFGFNV
                     EEGRFNEFKVAKQAEKRLEQSISYQKQKLNVFIHRLDNKIWPLQNELDVPRKFSLIEL
                     PEDLIVSSIYPSYFHEQGFVLRLANPTEQEKIVPEAILSLGTVVNALENKQELTTIPP
                     YDYLSILINER"
     gene            complement(15990..17039)
                     /locus_tag="SCJ86_001311"
     CDS             complement(15990..17039)
                     /locus_tag="SCJ86_001311"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373813.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS fructose transporter subunit IIC"
                     /protein_id="HEG1168884.1"
                     /translation="MEFKKQGREILNHFQSGVSYMIPLVVAAGLLTSIAVIFGGTGVW
                     DQTDTFWGVLRMIGQTGLQFIVPMISAYIAYSIADRPGLAPAFITGMMCQNLGMGFIG
                     GMVAGLACGYLANALKKVKVPMQVISLKSIMLIPFVTTLVVGIILWYVLATPIQAMTT
                     GLTNWLEGMSGANAALMSAILGGMMAFDMGGPINKIAYAFGVASFSSGSFGPSTAMLL
                     GIAIPPFGMFLATILNKKLYDESERDNAKTAMIMGLVGITEGTIPFAVKDPLRVIPSI
                     VVGTAVACGLNGFFGVTQETMLSTFMAIPFVTNIPLYLVSIVIGGVVTALMVNALKSI
                     KYKKEQQVNSVEEGK"
     gene            complement(17065..17379)
                     /locus_tag="SCJ86_001312"
     CDS             complement(17065..17379)
                     /locus_tag="SCJ86_001312"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373816.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS fructose transporter subunit IIB"
                     /protein_id="HEG1168885.1"
                     /translation="MTKIVAVTACIAGIAHTYMAKANLEKFAKKKGLDIKVETQGAMG
                     IENKLSISDVSEADVVIFAVDTNVSERGRFDGKPILTVGTSEVVKDGEETIDEAIALI
                     NK"
     gene            complement(17395..17841)
                     /locus_tag="SCJ86_001313"
     CDS             complement(17395..17841)
                     /locus_tag="SCJ86_001313"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302514.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HEG1168886.1"
                     /translation="MISEKININNIVIDLNASDKKSVVEKLSKLLETNGFVSDHKQFQ
                     KDVFDRENHMTTGIGKNIAIPHGKSLAVIESTVAVAKLKRSVEWGALDDQPVDLVFLL
                     AIKDTDKGTNHLRILAELSGRLMDDEFVKRIKDSRNKKELVKALSF"
     gene            complement(17842..19764)
                     /locus_tag="SCJ86_001314"
     CDS             complement(17842..19764)
                     /locus_tag="SCJ86_001314"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343132.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription antiterminator"
                     /protein_id="HEG1168887.1"
                     /translation="MGIEFSERQMKILYFLIENEHTTYKELSEQLYVSTKTISKDILI
                     LKEIIQKMPDVQMLVQPKKGVSLIGKKETLRNLLSSHKINIADSPEDRLTYVFAKLLN
                     TNDSLKIQNLADELYVSRSTVESTMKDVRKRLNKHGMSLVSDRNGVRIKADEKSKRRL
                     MSEVVNYYWGGFTASNSKTDLLDLNINFIGEANNFLNKDILKKVAELLNRYMNKTQLK
                     FTDYEFQTLAIHLAIAIERIETSNFLSRSNNSEEEISKNTLILIKMIEKAFSIIVPID
                     EQAYVDIHITSIEKNAMNHDLDQTKLSYLDMGVELQELVRIKLNEFYPDEELINNLVL
                     HLNAAVKRLKLGVNIYNPYTDKIKYSFKRSFMISVDLLEEIEERFCIHFNEDEIAYVT
                     LHIQSLLDRYKPDKTKVILVCSSGYGTSKLLEQRINNGFADMVEIEDVLSINELQDRK
                     VTDELVISTIPIENTNFPVIVVSPLMLGADVRKVKQYLQLNEQTEDAFLNLLDKRFCF
                     FTHQIKDNQVNVLNIITKKLVKENYAKEGILEGAIKREKLSSTAMNDFAMPHIEVDFI
                     KKPVICVYVNPEGIDWDGTLVHSVFFFALNEKVKPELNKIYETFNEIASNHELLANLK
                     TSSSFEKLVYILKEGL"
     gene            complement(19976..20872)
                     /locus_tag="SCJ86_001315"
     CDS             complement(19976..20872)
                     /locus_tag="SCJ86_001315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314301.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing histidine kinase"
                     /protein_id="HEG1168888.1"
                     /translation="MIKWLLLLVVIFMGSFIFFLQREIKRLTKKIATLSERQEYGGRL
                     SVEFREKNLMDLVDALNTWLDENEQKGQAFQEMEENIRLSIVGLSHDLRTPLTAINGY
                     VQLLEQVEDTEKREKYLTIIKQSVARLLSMTDQFYDLARIETKQKEMDLHTLSFSKEV
                     EEVFFSFYEQFSEKNIEVAFAEHSINTTVLADPLMLLRVLQNIIQNLLRYADGQAKVT
                     FSKEDPFIRLTVANPIKPGTQTSVEKVFQRFYTENTSRTNTESSGIGLYLSKKLVEGM
                     GGEMTARLDGGIFSISVKLRRV"
     gene            complement(20865..21545)
                     /locus_tag="SCJ86_001316"
     CDS             complement(20865..21545)
                     /locus_tag="SCJ86_001316"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287499.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HEG1168889.1"
                     /translation="MVLLETILIIEDDEAVHSLLEEVLEQRYKLLDAYSGTEGKLLLS
                     TYPVDLILLDLMLPGLSGEALLAEIRQTSNVPIIVLSAKSDQRDKVSLLAAGADDYVT
                     KPFDIEELLLRIGIQLRHQTSVQVKDLSQRIYYKEILLDKESRDVNVNGVAVHLTGRE
                     FDLLKLFLEHPKKVFSRANLYETVWQEPYFDSEKTVNMHISNLRGKLAKAHAVYIHTV
                     WGVGFRFD"
     gene            complement(21546..22328)
                     /locus_tag="SCJ86_001317"
     CDS             complement(21546..22328)
                     /locus_tag="SCJ86_001317"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287500.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEG1168890.1"
                     /translation="MFNLLTAEKIKVWKSRKMWVSLGLMVVLPLFYSWNEWYMHTKYG
                     NELNQATDTVINGATGILMVEKMAAWILLAFAAFACFYIGEEFQNGTIRNTLSLGRNR
                     MTYYVSKLLVTLLITTLGVVLITGLAMLAYTLAFGFGEVEGIKNYGNYVLKVFPVLLL
                     LTLGTLSVPVALTFITRSTSVSLLLSFLYIMGTAFIPGVFAKIKGLEFLTEWFTETWL
                     MYTDFAQQATYSQAPKMILVSLVTIVVSSALGMFIFQKSDIK"
     gene            complement(22321..23238)
                     /locus_tag="SCJ86_001318"
     CDS             complement(22321..23238)
                     /locus_tag="SCJ86_001318"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HEG1168891.1"
                     /translation="MDFGNETVLKATGITKKYGAAKALDKVSIEIKRGMIYGLIGENG
                     AGKSTFMRTIMGLISIDEGSIELFGTTDLQAARRRMGQSIETPALYPELTARDNLRIQ
                     AANGGVSDREIEDLLKMMRLENTGKKKAKNFSLGMRQRLAIANALITNPEFLILDEPT
                     NGMDPAGMAEMREIIQRLVKERGITVLLSSHLLDELSQIATHYGILHEGHLIKELSKE
                     ELAQESRQFIKIDTSATEQAVTVLDSLGYRDYFVQSSRVIQLFEGIDQVAAINQALVE
                     AKVPVDGIHLVGQKLEDYFLQLTGGNPHV"
     gene            complement(23402..23725)
                     /locus_tag="SCJ86_001319"
     CDS             complement(23402..23725)
                     /locus_tag="SCJ86_001319"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305527.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEG1168892.1"
                     /translation="MFSFAFYMVWLQREVPTHIQGRLMSTITTITMVASPLGLALFGA
                     LFDWKTTQPRLRDSLIFLGGSLAIVILITGLVKLLKLDLKEAKIFSEEEYRETTLVEK
                     KESGN"
     gene            24513..27740
                     /gene="fss3"
                     /locus_tag="SCJ86_001320"
     CDS             24513..27740
                     /gene="fss3"
                     /locus_tag="SCJ86_001320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010729285.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fibrinogen-binding MSCRAMM adhesin Fss3"
                     /protein_id="HEG1168893.1"
                     /translation="MYKKQKKKLCVLTILSLAVQAIVGAIAPTITFADEITHPQTVTV
                     ELDLAHQYAVEGTFSDGRPMSEVTVPHYAVYNGFKQDIFCIEPGVPIYNEFTPGYEKN
                     PLPDMSDKAKLVSVLWKKVGTDADTHIVAQKMIWQEVNGYTLHSIKRSDGSAVNIAAI
                     EAKINQAIDDYQKKPTFHNSTAKTVLGQSTTVTDTNNLNLSEFDEVVENAANIDYRVN
                     GNQLIITPNANSKESGVLTLKKSAGTGTPVAYKKAGQQTLMAGAIDKPNTYTIKIDVE
                     TEGSLKIKKVDKESGDVVPGTVFHLDFGKTLPAKDVTTDKEGIATLDEIPHGTKVTIT
                     EKSVPAPYTIDTTPMTTTIKAGETIYVTSKNAREKGQIILDKSGVETGSDLWNDNYSL
                     AGNTFAIRKDSPTGEIVQEMTTDENGHAETPKEIANALELGTYYVTETKASHGFVNTF
                     KPVKVELKYANQTVAPVTSNVKGQNQEVTGETTLTKEDKDTGDKAQGKAVFEGAEYTL
                     FTAKDGKAVKWSEAFKPELAKGTKGSDETVTLTLDEKNQVAVKHLAINEYYWQETKAP
                     EGYSLDETKYPVSIKKVDDSEKNAVITRNVTAKEQVIRFGFDFFKFAGSAAGTTETGF
                     NDLTFKVSPLEGTNEITGAEDEATTAYNEQLGFDGYGKFENLPYGDYLLEEVEAPEGF
                     QKITPLEIRSTFKENKEDFVKSEYVFTITEQDQKQPIKTVTVPYEKLTNKAFSVSLNR
                     LMLYDLPEEEDSLTSLATWKDGNKELTSLDSTELVDKLSYNLHEIKEDWYVVAQAIDV
                     DATKAAQEKDEKAKPVVIAETSATLANKEKTGTWKIQHKLTAEQVLNKTIVLFNYVYE
                     NKEAFEAGDKPVAKDVSLNNQAQTVSCTVEHHVSIQTKAHLENGSQTFTHGDVVDMFD
                     DVSITHDVLDGSKEAFETILYALLPDGTNKEIWKSGKIDYEVNDKEFTKTVLAKKVDT
                     GKYPEGTKFTFAEINYDKDGNINGKHNEDLKEKSQTLTPKEVPTILSTPKQPETPTVP
                     SGSQESSPTVKTFPQTGEKNSNVLLFIGFTLIFATAGYYFWNRRN"
     gene            27853..28167
                     /locus_tag="SCJ86_001321"
     CDS             27853..28167
                     /locus_tag="SCJ86_001321"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016171806.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdcP family protein"
                     /protein_id="HEG1168894.1"
                     /translation="MELKFVVPDMAETFGKLSYAGEGEIITEGYGRNTTVIGRSYHLY
                     SSKQRADDIEVVVAAEAGEKDFDQDQPLKAVNPHLVAKGYEIENRGFTDYVLYVDDLV
                     KA"
     gene            28180..28554
                     /locus_tag="SCJ86_001322"
     CDS             28180..28554
                     /locus_tag="SCJ86_001322"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdcP family protein"
                     /protein_id="HEG1168895.1"
                     /translation="MRLTEGIVVDSGLTFGKLRFSALRREVRKQNEDGTIGNEVKERT
                     YNLKSSAQGRMIQVSIPANVPLREFAYDAEVELVNPIVDTVANYVFREGTTVNWFIKA
                     DDLVLKRQPNQGNPTNQNERKK"
     gene            28564..28899
                     /locus_tag="SCJ86_001323"
     CDS             28564..28899
                     /locus_tag="SCJ86_001323"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297364.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="damage-inducible protein J"
                     /protein_id="HEG1168896.1"
                     /translation="MEINVEHILDCLDQYGKGELTEEQLVKALTSDEKMFLIIHQGLL
                     GENSDSEEDSDVLDWLGEEESFFIVVEVNENLCRQAESILEEIGVEMPDAIEGFLNQL
                     VETKQLPSS"
     gene            28982..30322
                     /locus_tag="SCJ86_001324"
     CDS             28982..30322
                     /locus_tag="SCJ86_001324"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002407829.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF87 domain-containing protein"
                     /protein_id="HEG1168897.1"
                     /translation="MYKGHRIRAGDQHLVYHFVLGWLLALFIGWMSVFYFQEFRQFDI
                     SKLSLSTIEIVWSIKDLVCLLGSLAFSGAMILLYIHFFLDHWRSLWHRQKLARMILEN
                     HWYEVKQTQSEGFFKDLNSSRTRETISYFPKICYRMKDGLLSIRVQISLGKYQDQLLK
                     LEKKLESGLYCELVEKELKDSYVEYTLLYDMIANRIGIDEVVAENGTLRLMKNQVWAY
                     DSLPHMLIAGGTGGGKTYFLLTIIEALLKSDAELFILDPKNADLADLGTVMPHVYSQK
                     EEISACVEDFYERMMARSKAMKEMSNYKTGENYAYLGLPPNFLIFDEYVAYMEMLTTK
                     ESAVILNKLKQIVMLGRQSGFFLILACQRPDAKYLGDGIRDQFNFRVALGRMSELGYS
                     MMFGEVEKNFFMKHIKGRGYVDTGGSVISEFYTPLVPKGYDFLDSISKLEKMND"
     gene            30400..31074
                     /locus_tag="SCJ86_001325"
     CDS             30400..31074
                     /locus_tag="SCJ86_001325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297358.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zeta toxin family protein"
                     /protein_id="HEG1168898.1"
                     /translation="MESNDSYLEYAKANKDKFISYIIKGKSPEVGRDAVFMAGSPGAG
                     KSEVAIGLAENYDNHVIIDADYFRTQFPDYNGKNSSLFQKASSWLVEQAFRYVLDHGY
                     SFILDATFAILSAEKNISRALKKHFRVTIFYVYQDPKIAWKFTQEREIAEGRHVPKKA
                     FINAFFKSRENIAKVKSKHPDVLLHIIIKDYTNNISEVHYATDNLELFLPLGYSRKDL
                     EVQLHD"
     gene            31067..31231
                     /locus_tag="SCJ86_001326"
     CDS             31067..31231
                     /locus_tag="SCJ86_001326"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297357.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEG1168899.1"
                     /translation="MTEKEQVTKIVKKYNKSIADLSENATAKEFKTVIKYVADQANEK
                     QRKLVGLNKK"
     gene            31307..32449
                     /gene="dcm"
                     /locus_tag="SCJ86_001327"
                     /pseudo
     CDS             31307..32449
                     /gene="dcm"
                     /locus_tag="SCJ86_001327"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016613853.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DNA (cytosine-5-)-methyltransferase"
     gene            32439..32729
                     /locus_tag="SCJ86_001328"
     CDS             32439..32729
                     /locus_tag="SCJ86_001328"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEG1168900.1"
                     /translation="MVYDELIGEIYWVIGKIQSDPELEEQLRRLKFDIRKNGVKVAGD
                     PYLMNEETDARIEINQVIAEFERIADQAKEPDIRQYLLEMKAELEVNGITDE"
BASE COUNT        10303 a         6409 c         5959 g        10414 t
ORIGIN      
        1 gataaataaa aaacaaaaaa agttccatct ttaaaacacg ctattctttt actttttctc
       61 acattctcta agaaaagaaa aggagaatga atttttgaca attcgagcaa aagtgattat
      121 caaatatgta aaatcagcta cttttttctt aaacgtttct ttatactaaa tataaataag
      181 tgtttcatga aaggagaact agatgaataa ttggcttgag tattttcctg aaaacgtttt
      241 ggaacgcggt tacagctatc atctgcacgg ttttgtccgt catttgaact atacaagcaa
      301 gtacttgtcc gcaacagttt ctggcacgga agattataag gtagtgatca cttgggatga
      361 aaagaccaac atgacttgtg attgtctata tgccatagaa ggaaaaaaat gcaaacacat
      421 ggctgctgtc ttattcgctt acgaagaacg tccaataaaa aaatccaact actcactttc
      481 cgaactctcg tcccttgttt cctccgctag ttcctctctt gtacgggagc ttttaacaga
      541 gattcttata gaacatccgc aatttatcga acgcttcaaa gttaagatgc catttcatgc
      601 aataaattat tcagataaat taacaactat catccataaa tacgatcata ttatcaagaa
      661 aaacaaaaac aggaaaacag cgaagttcat catggagatg cgaaagttca tccaagaagc
      721 agttgaatcg ctcattcagc aaaatgccta tcttcccgca ttcgaattga tcaatgaagt
      781 gattgctact ctcgaaacat tctattggga gcctgaggat gaacgaaccc ttcttctgat
      841 agaggactgt tattatttat ggaaagaatt attagcagaa gcacctcatg cagaaaaaag
      901 gcaaatgttc tcttggttcg tctgccaagt cgatcatact gacgcctctt attcaaaacg
      961 ctactccata aaaatcctga aagaagactt tcgggaaaaa gaattttcta accaaaagaa
     1021 aaaaatagat aaaaaaacta aagaaacggt aaagaaagac gatttcaatg aaaaatagca
     1081 agtaaacgat aacaaccatc ctaaatccac aagaaataca gcagaccttg ctttaaaaac
     1141 acgaatcatg caaaggatga agtaaagaag acttaacgcg tacccaatat tagtcatttg
     1201 actattaggt acgcgttttg ttttataagt catgacaaat ttccttcgtt gctgggtaga
     1261 ttttccggca ttgttgatag attttatgat acttttctac attttgtaag agaggttcca
     1321 ctgcatctgt atattggata aaattctgac aacaactttc aggtgtgtcg aaccactcta
     1381 atctttaaag ataaatatta aaataacgat taataatatt aacagtcttt atttttcacc
     1441 accaaaaacc tatctttgca tatactaaat agacgtatat acacagatag gtttgtcttg
     1501 tgaaaaataa tgattatcct taccaaaaaa tgtttgtttt tctacatgtt cgtacttatc
     1561 aaaaaatatg atccttattg aaattactgg ctatttatat tccaacgact tcttgtgtta
     1621 atttttgtta tctcatagta aattatgttt cattttcact taatttagtt gtcacgtgtt
     1681 cgtcaattcg taaaaacggt aaatatataa acacacttgc ggctataatt aatatttgta
     1741 aaacagctgc tcgccagtct ccggctgttg ctaaaaatcc cgatataact ggtggcgtag
     1801 tccaaggaat ttgcactact acatgattaa ttaatcccac agcagttgca aaatatgcta
     1861 ctgtcaaaga aaaaatcggt gcaagcacaa agggaataat cagactgata ttaaatacaa
     1921 taggcaatcc aaaaataata ggttcgctta tattgaatat agcgggagtt accgataatt
     1981 tagcaaagtc cttataatct tttctacgac tgaagataaa tattgcaatc aataaagcaa
     2041 tcgtatttcc cgatccaccc attaccgcaa aggtatcttt aaaggccata ttgataatat
     2101 gtggaatttg ctctttgttt gcataagctg ccatattttc ttgcatattc tgaatcaaaa
     2161 acggatctaa caatgaacct gatattactg cttgatggat accaaatcca aaaaatagat
     2221 tagcaataga tgtaattaac aaaaatcctg gtaaaccagt tgtaatttta cttaatggct
     2281 ctgtaactat cgtggtaatt agcttattta tatccatatt aaacaattga atgattataa
     2341 atgacaataa agcaaacgag gtaatcgtaa taatgattgg gattaataca ttaaaagact
     2401 ttaaaacagc gggaggaata tttccggata gctgaatctg tagtttctta ttattagaaa
     2461 gtttaataaa taattctgta gccgctaatc caacaaatat acctacaaac attcctgaag
     2521 ctcctgtata ggatataggt atcacacccg tcacttcaac agctttatcc gcattttcag
     2581 gagtaaacat catctgcata ggtgttaata caacaaatgt cgaaatagca actaataccg
     2641 ctgcaatatg atttgtataa tttctgcttg cacacatatg ataagatact gctgctacaa
     2701 ctaataatgt agttatgctt aatgtaccat tggcaattga agtaccaatt tgttgtaacg
     2761 tggtcaatgt actagtatcg acaatttccc ccatgaatcc tgttggttct aagatcactg
     2821 aattaataag tgtcacaaat cctgctaaca tcataaatgg cataaatgta gcaaacgcat
     2881 ctcgcaaagt tcttaaatgg atctgattcc ccaacttagt cgcaactata ccaaattttt
     2941 caaatacaat gtccttcctg ctacttttgc ccatattttt tctcctttat gcttattgta
     3001 atcgattact ttttccaccc aaataataca tcacttatca aaaaagtaaa tacttttaat
     3061 taaatatata tatctttttt agtataaaaa aataagctct gaaaaaataa ttttcccgag
     3121 cttatttttc aacaaaaaca gcttcaaaaa caaagtcttg atataaatgt ctcgataccg
     3181 aatactcgaa aggtttacca gacgatagag caacaacttt gtctatctca atcataaatt
     3241 cacctggatc tatttttaat atttttgcgt caaattcatt cgcagtatct cctttgatac
     3301 gtatgtgcga agtctttatt tccagaccca gttcatttgt gatataagca tatacagatt
     3361 tctttaaatg ttttggttcc aagcctggta tgactgctaa tggcatgaat gtttgctcaa
     3421 tcgagtatgg ttgacaatca ataaaacggc ttcgtacaat attatataca tagtctttta
     3481 cactgatact taatttatca gctacttttt tcgatggaag ttcaattgag aaagtatgga
     3541 tttctgaatc tacgtgttct tcaccaacca cgctaaacaa accttcgttt ggaccaagaa
     3601 ctcgtgagtt atcaattggt gttctgacga cttcagtccc atacccactt ctacttcgca
     3661 aaactccttc ggaaacaagt aagtctacgc ctttctttac tgttaacttg cttgtatgat
     3721 aaataactgc caattcatct cctgtaggaa gtttctcacc aattttatac tctccattta
     3781 caattttttt ctttatatct aagtaaattt ccatatactt cgcaattttt ttcaatggtg
     3841 accctccaat caaaaacata tcttcatcta tactttaaca taaataaacc gataaaatcc
     3901 acatcaaaat tatacacaca ataaaaaaga tatatatatt taaacaaaac tatttacttt
     3961 aagttgtaag cggtgttatc attaagtgga aacaggaggt aaaaaaatta tgacaactat
     4021 gaaaaagcta acttttccag atgatttttg gtggggttcc gcttggtcag ctgagcaagc
     4081 agaaggaaaa ggagaaacag gaaaagcaga gactatctgg gatcgctggt ttaaagaaca
     4141 accttaccgg ttttatcaac gaatcgattc ctctattaca accgatcaca tccatcaata
     4201 taaagaagat atagaactaa tgaaaaaaac tgggcataac tcctttcgcg tttctatttc
     4261 ttgggcacga atgtttccaa atggcggtac cggaaaagta aatcccaaag ccatttcttt
     4321 ctatcgaaaa ttattttccg aaatgaataa taacggaatc aaagtatttg ccaacttata
     4381 tcattttgac atgcctgcta ctcttcaaga taaaggagga tgggaatcac gtgaagtagt
     4441 tgatgcttat gttcattacg ctgaaacttg ttttaaagaa tttggtgact tagtttgtca
     4501 ttggtttacc ttcaatgaac cacttgggcc tattttaggt acttatcttg aagattttca
     4561 ttatccaaac ctcattgact ttaaacgcgg agcgcaagca gcattcttca ctatacttgc
     4621 acacgcaaaa gcaatacaag tatttaaaga actagctctt cctagtaaaa taggcgttat
     4681 tctaaatttg agtcctactt ttcctcgtag tcaaaatcct gctgatgtga aagcagcaga
     4741 aatcgctgat ttattttata cgcggagctt tttagatcct atggttaaag ggaatttttc
     4801 taaaaagctt atcctgttat tacaagaata taatcagctt cctgaagatt atacgaaaga
     4861 agatcttgta tgtatctacg aaaataccgc tcaaattctt ggactaaact attacgagcc
     4921 acgtcgcgtc aaatctcgtt cttctatggt ggatacatct agtccatttt taccagagtg
     4981 gtttttcgaa ccacatatca tgcctggacg acgaatgaac gaataccggg gatgggaaat
     5041 ttacgaaaaa ggaatttatg atttgtgcat ggatatcaag aaccattatg gaaacatcga
     5101 agcatttatc tcggagaacg gaatgggcgt tgcggatgaa gaacgtttca tggatgatac
     5161 agggcaaata attgatgatt atcggattga gtatatcaaa gatcatcttg cttacttatg
     5221 gaaggcaata aatgaaggat gtaacattaa ggggtatcat ctttggacat ttattgattg
     5281 ctggtcatgg atcaacgcct ataaaaaccg atatgggctg gtttcactcg accttactac
     5341 tcaaaaaaga acaattaaaa aaagtggcga attttacaag caattaagtg atacaaatgg
     5401 atttagatac gatgtagact tgttagttga ataatattct aaaattccac ttgaagtgaa
     5461 cacattagaa aacaagaata atgagaaata ccaataacaa atactttttc ttcatacttc
     5521 atttatactt gtcagtataa gagactgtga caaatgtagt gatcctcaga agttaggtct
     5581 ggaaatctaa cttgagaaga tttttttaaa ctacgtccag tcttttttat ttgtttaaaa
     5641 ttgaagttga caagtcttca ttaaaaattt agaatattca caagtttgac gacaattatg
     5701 aaaatttatg tcgtgctaaa ggagttttgt tattggatga aaagttagat ataaaaactg
     5761 cgatcaaaga cacgttacct accgtttttg gttatatcgg tattggactt gcatttggta
     5821 tcgttgggaa agctgccggg tttcatccgc tggtcgtcac gttgatgtcc ctgctgatct
     5881 atgctggttc tgcccaattt atcacggtca gcatgcttgc tagccatagt ccactgctct
     5941 ccatcgttct ctcgaccttt ctagtcaatt cccgaatgat tctgatgagt atgacgattg
     6001 ctccttattt caagaaaaat cggctgcttc aaaatctgct gattggcaca ttgctgacag
     6061 atgaaagttt cgcactggga atgaataaat tgaactatac tggacaaaag ttaaactttc
     6121 gttggatgaa tacagctaat tggatttctt atttgacgtg ggtgggttct tctcttgttg
     6181 gcgcactttt agggaatttt attactgatc cgaaaaagtt tggtttggat ttcgccatcg
     6241 tcgctatgtt tatcgggtta ctttatcttc aagtcatttc tgatagaaac acctctaaac
     6301 gattacagtt gatcctgatt ggtctcactt tgattctggt atatgtcgga ctgattttta
     6361 tcccaagtaa cttagtgatt ttagttgtaa cactgattgg gtgcggattg ggggtgtgga
     6421 tcaaacatgc cttcttttga gtttacattg ctgactattt taggttgtac tttagcaact
     6481 tggctttcta ggatcctacc atttgtctta ctgaaaaaat tcgatttgcc gcaaccgttg
     6541 cttgagtatt taagttttgt cccgatcgtc attatgtctg ctttatggtt cagcagtcta
     6601 tttacccaaa atatcggaca tctgccgcag atcaatctcg aaaatgcttt ggcttcagtt
     6661 cctacacttt tagctgctat tctttcaaaa agcttattgg tgatcgtctt ggtaggaatt
     6721 ctttcccttt cgttgattcg cttattataa ataaaaaaac aagagcttat ggcaaattac
     6781 tgtcatgcgc tcttgttttt atcaaagtca aataagaaaa aaatcggcgt attcttgctt
     6841 atccgttgaa cggaccacta acaacgactt ttcctaatat atgtcctgtt tctacgtctt
     6901 tggttgcttg tttcaagttc ttcgcattca tcccatctga atagaccttg ctcaatgtac
     6961 tgcggataat cccttcattt gctaaagcag caatgtgcgc taaagcttct ccttgcgttt
     7021 cgatttcata gttatagtct gttttagcga acatatactc ccagtcgaag ctgatagatt
     7081 ggtttttcca aacacctata tctaacggct ctttgatccc tacgatcgtt cccacatgtc
     7141 caaatggtcg gacaaggtgt ttgatttgat caaagtagcg ggtaatatcg aataatacag
     7201 cgacatactc tacttgatcg attcctaatt cacgaaaact tttacttaag tcgtgatgat
     7261 aatcgattgg atagtctaca cctgtttttt tcagccattc aaaattcttc ggacttgcag
     7321 tcgcgtagac ttctaggcca gcccaatgcg ctagttgatt caaaatcgaa ccgacgcccc
     7381 cagctccgtt gatcactaag atttttttcc cgcggttggc attttcttct ggagtcagac
     7441 caaatttttc aaataacaat tcataagcgg tcaatgaggt caacggcaag gctgcggctt
     7501 cttcatctga aagatttttt ggtgcaagag cgacaatacg ttcgtcaacc agttggtatt
     7561 cttgattgct tcctgctctt gtcgttgtac ctgcgtaaaa aacgcgatcg ccgacagaaa
     7621 atttttgcac ttcatcacct acagcaacaa ctttcccgac tccatcaaac cctaagatac
     7681 gtaaagcatt tcttatcccg ttatcttggc gaagtttcgt atcgacagga ttaacagata
     7741 ctgcgttgac ttttaccaga agatcccgtg aactgggtac aggtatctgt tctttttcat
     7801 caataaagct ttttggatca tcgatcgaca acccttcata aaaaccgact accttcatta
     7861 attccttttc tgacatacga attcctcctt agcttcatca agttggagca tccatacagt
     7921 tttcatttca atatatatag tatgctattt gttccacttt tattcatcat aattattctc
     7981 ttacttaaag taaactattt gcactttttt ggttaaaaaa gaggttgcga caagactttt
     8041 gtcacaacct cttttaaacg gtgttcaatc aactgaccct cggtattcgc tcaaaaaacg
     8101 acaaaatatg aggagctatt tttgtttggc tgttcttatt acttgatgca ggacagcttt
     8161 ttcacgacct ctaattcacg gtgttcaagc aagaaatcat tgttcactga aaagcgttgg
     8221 atgttctttt tcaaaggtgt ctaagatctc tcgtacttca tcagtagatt tcgtattcat
     8281 caactgaact cgcaagtcat ttgctcctgg aaatcctttg atatagattt taaagaaacg
     8341 atgtagtcct gtgatggaac gcgggatgat ttctgaataa tgatcttgca gatctagttg
     8401 cagacgaagt agtccgatca gttctttttc tgtatgttcc ctcggttctt tttcaaatgc
     8461 ataaggattc ttgaaaatcc cccggccaat catgatgcca tctacgccat atttttcggc
     8521 caattcttct cctgtctggc gatctgggat atctccgttg atcgtgatca gtgtttgcgg
     8581 agcgatttgg tcacgtaaag caatgatttt agggatgact tcccaatgtg catctacttt
     8641 actcatctct ttacgcgtac gcaaatggac agaaagattg gctatatctt gatgaagtag
     8701 gtgactgatc cattcttcca tctcagacat ttccgtatag ccgatacgcg tcttgacgct
     8761 gacaggcaaa ccgccagctt tagccgcttc aatcaattgt gccgctactt ctgggcgaag
     8821 gatcaagcca ctgccttttc cacgttctgc tacattaggg actgggcagc ccatgttgat
     8881 atcgattcct tggaagccca tttctgccat agcaatgctc atttctctaa aaaattctgg
     8941 cttgtctccc caaatatggg caaccatcgg ctgttcatct tctgtaaagg tcaatcttcc
     9001 tcgtacactg tcttttccat ctggatgaca gaaactgtct gagtttgtaa actcagtaaa
     9061 gaagacatcc ggtgctgctg catgctttac gacatggcga aatacaacat ctgttacatc
     9121 ttccattggt gctaaaacaa aaaaaggctt tggcagctct ttccaaaaat tagtagtcat
     9181 ttatttgtta ctctccattc tatcaagcag tgaagtcgca atctttctca aaaacattgt
     9241 cattatacta ggaaacaaaa aaagaagcaa agtgaacagc ttaaaagatg tttctgattg
     9301 agcaaaaaaa gactgtgaca aaaatcttat cacaattcct tttccggaat aaatggtgtt
     9361 caaaagcttg catctgcggt aataagtcca accaaacgaa aaataggagg agctattttt
     9421 gtttggctgt tcttattact tgatgcagaa cagctttttc acaacctctc cactaaacgg
     9481 tgttcaatca gctgaccctc ggtattagct caacaaacga caaaatatga ggaactattt
     9541 tcccgtttgt ttctctaata ctcaggtcaa aacgctgatt tcacaacctc ttttctaact
     9601 tctcaatcct cttcctttat tgatcaaata ccaagtaacg gcgtacaaaa caacgatgaa
     9661 tagcacaagt acgatcatcg atgttgctac tggcacatcg attgttccaa ggaatccgta
     9721 acggaaaccg gagatcatat aaacgatcgg attgactttc gaaacagctt gccaaaatgg
     9781 cggcaacata gagatggcat agaaaacgcc acctagataa gttaacggct gcaagacaaa
     9841 tgtaggcacg atcgatacat cgtcataaga ctgagcaaaa atcccgttga gcagtcctgc
     9901 taacgaaaac aagattgccg tcatcaataa tgtaatgatg accatgaacc atgaatacac
     9961 atgaagtggt acaaagaaca aggaaatgat cgttacgaga gagccgacga gaatactgcg
    10021 acccactccg ccaatcacaa agccccaaat gatcacatgc gtaggaacag gcgctaccaa
    10081 gatttcttcg atgtttttct ggaatttttg agagaagaag gacgaagaaa cgttggcata
    10141 cgaactagtg atagcagaca tcataatcaa accaggcacg atgaattcca tatatgaaaa
    10201 accacccatg tcaccgatcc gaccaccgat catttttccg aaaataacaa agtacaagga
    10261 tgtcgtgatc acaggcggaa ccagtgtttg gacccagata cgcaaatagc ggttcgtttc
    10321 cttgatagct aaacttttta atgcagtaaa ataaagatta aacatttttc tcctccgtat
    10381 gattattttc ttctgtgatt ttcaaaaaca gttcttctaa acgattcgac ttattcctca
    10441 tagaaagaac tttgattcct tgtcttgtca gctgatcgaa gattccgttc accccttgat
    10501 ttcgttcaac ttcgacagcg attgtctgtt catcttcaaa tgtacaagcg tatccttcaa
    10561 tcacaggttt ggtctcatat ggcgctaaat caaagataaa tgtttcaaat tgcagtttgg
    10621 atagaagctc tttcatacta gtattctcga tcagttcacc tgattggatg atcccgatgt
    10681 tgcgacaaag catttcagct tcttctaaat aatgcgtcgt caaaatgatc gttgtaccat
    10741 tttcgttcaa ttctcggaga aactcccaca tctcgcgtct caattcaata tcgactcctg
    10801 ctgttggttc atccaagatc agcaaacgtg gttcatgcat caatgcccta gcaatcatca
    10861 gtcttcgctt cattcccccg gaaagcatac gtgcacggac atttctcttt tcccaaagat
    10921 tggactgctt caaatatttt tcgctgcgtt tcatcgcttc tttgcgagag acaccgtagt
    10981 agcctgcttg attcacgacg atttgctgga ccgtttcaaa gggattgaaa ttgaactctt
    11041 gcgggactaa tccaatctgt tgctttgctc ggactagatc cgtatccaga tcataaccga
    11101 agacattcac ttttccagat gttttattga ctaaagaagt aatgattccg atcgttgtag
    11161 acttcccagc tccatttggt cctaatagcg catagaaatc tccttctttc acggaaagat
    11221 cgattcctcg caatgcttcg accccacttc cataaacctt tttcaaatta tttatttcaa
    11281 gcgcatttgc catttatttc ctatctcctt atagacatga ttctttattg tatcatggaa
    11341 tacagcagat gaaaaaacaa cttggttcca gcgaaaaatc aggttgctgg gctcaggaaa
    11401 atctcctaat gaaagacatc aatcggacga agtcagacaa ccttttcaga aaaaacaaat
    11461 cgaagataaa ggattactat tcatatgtgc aacaaaagga gcgtgagaca aaagtaaaga
    11521 acacttttgt ctcacgctct aaacacgtat aaacggcgga agcaaaagcc aagatttttg
    11581 atccaataaa tactttcgat cacttcgcct atcccaatac atgccactgc cgcttttcat
    11641 caaataacgc ggcttccgtt tttgcggttt ctactaaagt ctctgggtaa tccaaaactt
    11701 tcagcaaggc aatcgcattt cgggtccttg aaggtccttc ttttaatttg aaatcaaaac
    11761 tgattccttt tccttctgtt acttgttctt caaagtggac attctcacaa taattcttta
    11821 aaatctctgt caattcgata tcatgtgtgg cgacaaatgc taaagatgga tactccgcaa
    11881 gccaccgaac gactgaagcg gaagaagcaa tccgttcgat cgtatttgtc ccttttaaaa
    11941 tctcatctac gaagcagtag cagcgttctt tggaagccac ttggtccaac aaacgtttga
    12001 tcgatttgat ttctgaaaca aaataactgt cgccttcaaa taaatcatct tcgattgcca
    12061 tggaagtcaa cacatgtcca ggttgcaggg taaactgttc tgcaatcgct gtttggatcg
    12121 tctgtgctag aatacagctg atggctatac ttttgacata cgtagatttt cctgatgcat
    12181 tggaacccgt gactaatgta ttcttctgcc agttgaccgg attcactacc gcttctttta
    12241 acaatggatg atagatatct ttcgctgtca caccgccctc agtaaactct ggtcggcaag
    12301 tgatgggcat ataagtacgg taattcaata ttgcagcggc tacttctaat tctcccagca
    12361 attcccacag cttgatggct tcagttgaat gcttttctaa tttagtgaga acgaaatgat
    12421 aggagataaa tggcagcatg aacatgatgt tgacatattc aaaaatcaac tctgcttcac
    12481 tcccactctt catacgaaat gaaaaaccta atttagggat gtctcttaat ggtttagttt
    12541 gctgtttcag ctcttcttga agtggtgtcg gcagttttgc tagctgattg gcggctgaaa
    12601 tcgttgaaac caaataactc atgctattca gctctgtttc tagtgtttgt ttcttcacta
    12661 gataatagac cgtattgaat actaacccac cgattgccaa atagattcct agtacttgtc
    12721 ctaaaagaag caaacccaaa cccaccagcg gaagcaagcc aagaaaaata aataaaccta
    12781 cgcgtccaat ctttttactt tttccatccg ctagatattg ttttgcaaaa ttatgatctt
    12841 tttttcctaa acgagcgaat tgatattgga ttttttctct cagttgagga ttttcctgat
    12901 aaaaagcaat cagtttttct aactgctgat cttcaccaaa atcaaagctg cgtaaacgtt
    12961 gatacaacgc ttctgaacct acactagaat aggtactgtt gattccttcg aatacttcga
    13021 acatatccag gtcataccaa gtaagatcat cgatttcgct atcccatgaa cggaacttct
    13081 tctccgtgag ccatgcttcc ttcaagcttt cttctttatc aaatctcggt tgataaggaa
    13141 ttttttccca tttgctacga actgtttcct ttaatttcaa acgtgaaaaa atgtctaaag
    13201 caactaataa aataacagct gcaataataa ttaaaacgat aaattctctt tgacccatat
    13261 ccctctccgt tcctttattt gttagcttta gtatagcact gttgcgctat ttgaaaagag
    13321 ggtaaaaaaa cacctttcaa aataaaaggt gtttttttct tgtatattac atatttatct
    13381 ctcatttatc aaaattgaca aataatcata gggaggaatt gttgttaatt cttgtttatt
    13441 ctccagtgca ttgacaactg tcccaaggga aaggatagcc tcaggtacta ttttttcttg
    13501 ttctgttgga ttagctaagc gaagaacaaa tccttgttca tgaaaataag aaggataaat
    13561 cgatgataca attaagtctt caggtaattc aatcaatgaa aatttacgag gaacatcaag
    13621 ttcattttgt aatggccaaa tcttgttatc taacctgtga ataaatacat tcagtttctg
    13681 cttttgataa gaaatagatt gttctaaccg tttttctgcc tgttttgcca ctttgaactc
    13741 attaaatctt ccttcttcta cattaaatcc aaaagtgact ttatactcac ctatctcttg
    13801 agctagcggt gtttccatca tgacatgccc ttcatttgtc gtatctcctg aagctcgccc
    13861 aggtcgccaa gcaagattag gttttccaag ttcccctgtt gttgaaaata aggttatgat
    13921 taattgattt tctatacgtt catactcttt taaaccatct gcataaaacg taaaataatg
    13981 atcattttct gccacagaga cactcttatc gaatatttct aaattaacag gtttctctac
    14041 catagtttct gaccaattcg gaggaatgac atcaggggta ttttcaataa atccgttttg
    14101 aatttgtgca attgattttt cagatttatc taaaacatta attgctaagc gcatacgatg
    14161 cgagtaaaca gtattatgaa ttttgagtgt gccttcaacg atttgacttt ctcccagact
    14221 gaacgatatt tcataagata tttcttccat ttcgggtttt tcagataaac gatcttttaa
    14281 atctttagga agttgagctt ttccgtaaac aactaatgtt tcttgtaaag aatctttata
    14341 tactttagct gtttctaatt ttagagatag ttctgtatct ccttctaatg gtgaataatc
    14401 atacgtatcg ccatcatttg ctgaatcgat caaatgaacg aaatcaagat attgtctatc
    14461 tccaactatc aaattcaatt gcccatcttt aaaagaaagt ttatatttat tgttacttat
    14521 ctcagttttg tctatctcga ttgctgtttc taatttttca ttatttttct caaattcaac
    14581 aacagtgtat cctagtgctg gcacatttac atttattttt atgtcaagtt cataataagc
    14641 tggttcatca aaatattcga aacctgtagc aatcatccga taagcatttt tccgttctgg
    14701 aaaataaact tctttttcaa tgatattata gggactacct ttgaagtgta ttttctttga
    14761 atcagctact acatgcacga ttttcgatcc actataattc tcagtcgttg tgttgaaaac
    14821 aaggacttca ttcgatttta gctttaatgc atcagccatc cgttttacaa taaggttttc
    14881 gattccgtca gctatctcat tagcttcttt gattcgatgg aaaatatctt ccgcaacatt
    14941 atctgaaaca cagccaccaa tactatcatg agcctggttt tgcaaaatct ttttccataa
    15001 ccgacgaagt aggcctttac tgatatggat accatttctt tctgcaatca gcatgagcgg
    15061 ctcaactcgt tttagaattt tttgttccaa tttaaaattt tctattttca tttttgttct
    15121 tactgagcca atagaacgat gaacacgaga gtaaaccggc tgacgaagtt cacctttata
    15181 ttcaggtaat tcttttttgt ttctgataac ttctacataa gaaggataat ctgaaataat
    15241 attgtggaat ttactgtatt tattcaactt ctctacgact tgatcaaaat ttaaaatcat
    15301 tgacttttga tctacacccg aaggtactaa aatatctttg ttttttcctt gcatatgaag
    15361 catttcaact gcttcatcca acttttcttt tgcaaattca ggaatatctt taattgcgtc
    15421 taaggtcatc attccagtca tataaccaaa tggaaaattc atggcataaa caaatttatc
    15481 tcccaatcct ttccagataa aataaggaga tggaaccatc gtttcaaagt ttaatcctcg
    15541 ccaacacatg aaattatcga tttctacttg atgcaaaagt gtaggcaaat tcgcattgaa
    15601 cccaaacgtg tcaggcaaat accctaccat cattggatct ccatatttaa ggatcgtatc
    15661 actgattcca atcattaagt ttcttaaaat tgattcagca tccactaata aggcatctgt
    15721 ttgtgcatac cacggtccca cgaatagttg tcctgttttc atcagtcgtt ggataacctt
    15781 aagcttttgc ggattgatct ccaaatactc atcgacgatt gaagtttgtc catctaaaca
    15841 aaagtttact ttagggttcc tttccaattc atctagaact tcagtaaaga cctgatcaga
    15901 caatacgatg gcatcttcat ttgtaaaata ccattctctg tcccagtgcg tgtgttgaat
    15961 tatatgtgca tttaccatat cagattctcc tactttcctt cttctactga atttacttgt
    16021 tgttcttttt tatacttgat ggattttagt gcattgacca ttaacgctgt aaccactccc
    16081 ccaataacaa ttgatactaa atataaaggt atattcgtaa caaaaggaat tgccataaag
    16141 gttgaaagca ttgtttcttg agtaacacca aaaaatccat ttagaccaca tgctactgca
    16201 gtgcccacca cgatgcttgg tattactcgt aaaggatctt tcacggcaaa cgggatcgtc
    16261 ccttcagtaa tccccacaag gcccataatc atagctgtct ttgcattatc tcgttccgat
    16321 tcatcataaa gttttttgtt taaaattgtt gctaaaaaca ttccaaatgg cggaatagca
    16381 attcctaata acatggctgt actaggtcca aagcttccgc ttgaaaaaga agcaacccca
    16441 aaagcatatg cgattttatt gataggtcct cccatatcaa acgccatcat tcctcctaaa
    16501 atagccgaca tcaaagcagc atttgctcct gacatgcctt ctagccaatt agtcagtcct
    16561 gttgtcattg cttgaatagg cgtagctagt acataccata atatgattcc aacaactaat
    16621 gtagtaacaa aaggaatcag cataattgat tttagactaa taacttgcat cggtactttt
    16681 acttttttca atgcattagc taaatagcca caagctaatc cagcaaccat tcctccaata
    16741 aatcccatac caagattttg acacatcatc ccagtgataa aagctggtgc tagaccggga
    16801 cggtcagcaa ttgaatatgc gatataggct gaaatcatcg gtacgataaa ctgtaatccc
    16861 gtctgaccaa tcattcgcag gactccccag aatgtatccg tctggtccca aacacctgtg
    16921 ccaccaaaaa tgactgcaat agaagttagt aaccctgctg ctacgacaag cgggatcata
    16981 taggaaaccc cactttggaa atgatttaga atttctcttc cttgtttctt gaattccatt
    17041 agtaactccc ccacgatata ttttttattt gttaattaat gcaattgctt catcaatcgt
    17101 ctcttcacca tcttttacta cctctgaggt ccctactgtt aaaatcggtt taccgtcaaa
    17161 gcgacctcgt tctgaaacat ttgtatctac tgcaaatatg acaacatctg cctcggaaac
    17221 atccgaaatt gaaagtttgt tttctatccc catagccccc tgtgtttcta ctttaatatc
    17281 taaccctttc ttcttcgcaa acttttctaa attagctttt gccatataag tatgggcaat
    17341 ccctgcgata catgctgtta ctgctactat ttttgtcata tgaaattcct ccttttaaaa
    17401 tgatagagct tttaccaatt cttttttatt tctcgaatct tttattcttt taacaaactc
    17461 gtcatccatc aatcttccag ataattctgc taaaatacgt aaatgattag tacctttatc
    17521 agtgtctttt attgctaaca aaaagacgag atcaactggt tgatcatcta atgcgcccca
    17581 ttcaacagac cgctttaact tggctacagc cactgttgat tcaattactg ctaggctttt
    17641 gccatgagga atggcgatat ttttcccaat tcctgtggtc atatggtttt ctctgtcaaa
    17701 tacgtctttt tgaaattgtt tgtggtcaga cacaaaccca ttagtctcta acaatttcga
    17761 taatttttcg actacacttt ttttatcaga tgcatttaaa tctatcacta tattatttat
    17821 attgattttt tcactgatca tttataagcc ttctttcaat atataaacta atttttcaaa
    17881 cgacgaactc gtttttaaat ttgctaataa ctcatgattc gaagcgattt cattgaatgt
    17941 ttcataaatt ttattcaatt caggttttac tttttcatta agtgcaaaaa agaaaacaga
    18001 atgaacaagt gtcccatccc aatcaattcc ttctggatta acgtagacac agattactgg
    18061 cttttttata aaatctactt cgatatgcgg catagcaaaa tcattcatcg ctgttgaaga
    18121 tagtttttct cttttgattg ctccctctag tatcccttct ttcgcgtaat tttcttttac
    18181 caattttttg gtaattatat ttaaaacgtt cacttgatta tcctttattt ggtgggtaaa
    18241 aaagcaaaat cgtttatcta ataaatttag aaaagcatct tctgtttgtt catttaactg
    18301 tagatactgt tttacttttc tgacatctgc ccctaacatc aaaggactaa caacaataac
    18361 aggaaaattt gtattttcaa tcggaatcgt gcttataaca agttcatcag tgacttttct
    18421 atcttgtagt tcattgatgc ttagtacatc ttctatctca accatatctg caaaaccgtt
    18481 atttattctt tgctctaata attttgatgt tccatatccg ctactacaaa ctaatatcac
    18541 tttcgttttg tcaggtttgt aacgatccaa aagtgattga atatggagtg ttacgtaagc
    18601 gatttcatct tcattaaaat gtatacagaa tctctcttcg atttcctcta ataaatctac
    18661 acttatcata aatgaccttt taaaagaata tttgattttg tccgtataag ggttatagat
    18721 atttactccc aatttgagtc ttttaactgc tgcattgaga tgtaatacta gattattgat
    18781 caattcttca tcaggataaa attcatttaa tttaattctt actaactctt gtagctcaac
    18841 acccatatct aaataggaaa gcttcgtttg atctaagtcg tgattcatag catttttttc
    18901 aatcgaagta atgtgaatgt caacataagc ttgttcatct attggcacaa taattgaaaa
    18961 tgctttttca atcattttga ttaaaataag tgtattcttt gagatttctt cttcagaatt
    19021 attactacgt gataagaaat tgcttgtttc aatcctttct atcgcaatag caaggtggat
    19081 agctaaagtt tgaaattcat aatctgtaaa tttcaattgt gtcttattca tgtatcgatt
    19141 aagcaattcg gctacttttt tcaatatgtc cttatttaag aaattattcg cttcaccaat
    19201 gaaattgata ttcaagtcta agagatctgt tttactgtta gatgctgtaa agccacccca
    19261 ataatagttc acaacttctg acatcaacct tcttttactt ttttcatcag ctttgatgcg
    19321 cacgccattt cgatctgaaa ctaaggacat cccatgcttg tttagtcttt ttctaacatc
    19381 tttcattgtg ctttctaccg tcgacctgct cacgtataat tcgtcagcaa gattttgaat
    19441 ctttagagaa tcattagtat tcaataattt agcaaacaca taagttagac ggtcctccgg
    19501 agagtctgcg atattgattt tatgactgga taataaattt cgaagtgttt ctttttttcc
    19561 aataagcgaa acgccttttt taggttgaac caacatttgt acatctggca ttttttggat
    19621 tatttctttc aaaattaaaa tatctttgct aattgttttc gtagatacat ataattgttc
    19681 tgataattct ttgtaagttg tgtgctcatt ttcaatcaaa aagtatagta ttttcatttg
    19741 ccgttcgcta aattcgatgc ccattatctt cacctcctga atacagctta agtgtacaat
    19801 tattatctct attttcatac agaagaaagt tcttgattaa tgtagaagtt tctaatattc
    19861 ttgattattt caaatcaata aaaaagcctg caatatttat atcacaggct tttttctcta
    19921 ttaaatatat ataagataat acatttttct actagttctc actaatcttg tataattaaa
    19981 ccctccgcaa cttgacggag atggagaaga tgcccccgtc taatctagcg gtcatctctc
    20041 ctcccattcc ttccaccaat ttttttgaca ggtaaagccc gattccgcta gattccgtat
    20101 ttgtgcggct ggtattttcg gtataaaaac gctggaagac tttttcaaca gaggtttgcg
    20161 ttcctggttt gataggattg gccactgtta atcgaatgaa tggatcctct tttgaaaatg
    20221 tcacctttgc ttgtccatct gcataacgca ataaattctg tatgatattt tgcagcactc
    20281 gcagcaacat gagcggatct gctaatacag ttgtatttat cgagtgttca gcgaacgcta
    20341 cctcgatatt tttctcagaa aattgttcat aaaaagagaa aaatacttct tccacctctt
    20401 tagaaaaaga aagcgtatgg agatccattt ctttttgctt cgtctctatt cgagcaagat
    20461 cataaaactg atccgtcata ctgagtaatc ttgcaacaga ttgtttgatg atcgttaaat
    20521 acttctcccg tttctccgta tcctctacct gttccagtaa ttgtacataa ccgttaatag
    20581 cagtcaaagg agttctgaga tcatgagaaa ggccgacgat cgatagtctg atgttttctt
    20641 ccatttcttg aaatgcttgt cctttttgtt cattttcatc caaccatgta ttcagtgcat
    20701 ctactaggtc cattagattc ttttctcgaa attcgactga aagacgacca ccatattctt
    20761 gtcgttcaga aagagtggcg attttcttag ttaatctctt gatttccctt tgcaggaaga
    20821 aaataaaact tcccataaag ataacaacga gaagcaacag ccatttaatc aaatctaaac
    20881 cccactcccc acaccgtatg aatatacaca gcatgtgcct ttgctagctt ccctcgaaga
    20941 ttggaaatat gcatattcac tgttttctca ctatcaaaat atggttcttg ccaaactgtt
    21001 tcataaagat ttgctcgcga aaatactttt ttcggatgtt ctaaaaaaag cttcagcaga
    21061 tcgaattctc ttcctgtcaa atggacagcc acaccattca catttacatc cctcgactct
    21121 ttgtccagta agatttcctt gtaatagatg cgttgcgaca gatcttttac ttggacagaa
    21181 gtctgatgac gaagttggat gccgattcgt aacaaaagtt cttcgatatc aaatggtttc
    21241 gtcacatagt catccgctcc tgctgccaac aaggaaactt tatctctttg atcgcttttt
    21301 gctgaaagaa cgatgatcgg aacattcgat gtctgtcgga tttctgctaa caatgcttca
    21361 cctgaaagcc ctggaagcat gagatctaga aggatcaaat cgacaggata tgtgcttaac
    21421 aataattttc cttccgtacc tgaataagca tcaagaagtt tgtatctctg ttcaagaacc
    21481 tcctccagta aggaatgaac agcttcatcg tcttcgataa tcaaaatagt ttccagcagg
    21541 accacctact taatgtcaga tttttggaaa ataaacattc ctagagctga tgataccacg
    21601 attgttacta gactgaccag aatcattttc ggtgcttgcg aataagttgc ttgttgcgca
    21661 aagtccgtat acatgagcca cgtttccgta aaccactccg ttagaaattc caagcctttg
    21721 atttttgcga aaacaccggg aatgaaggcc gttcccatga tataaagaaa agaaagaagc
    21781 aaactgacac tggtacttct agtgatgaat gtcaaagcca ctggaacaga taatgtccct
    21841 aatgtcagca gaagcaatac aggaaatact tttagaacat aatttccata gtttttgatt
    21901 ccctcaactt caccaaatcc gaaagcaagt gtgtaagcaa gcatggctag tcccgtgatc
    21961 aaaaccacgc caagtgttgt gatcaaaagc gtaacgagaa gtttcgacac ataataagtc
    22021 attcgattcc gtcctaaaga taacgtattg cgaatcgtac cgttttgaaa ttcttcccca
    22081 atataaaagc acgcaaatgc tgcgaaagcc agaagaatcc atgcagccat tttttctacc
    22141 attaaaatcc ctgttgcacc gttgatgact gtatccgttg cttggtttaa ttcattgccg
    22201 tattttgtat gcatatacca ttcgttccaa gaataaaaaa gcggaagtac gaccatcagt
    22261 cctagtgaga cccacatttt acggctcttc catactttta ttttttcagc agttaataga
    22321 ttaaacatgt ggattgcctc cagttaattg taagaaataa tcttccagtt tttgcccaac
    22381 taggtgaata ccatccacag gaacttttgc ttctaccaat gcttgattga tggctgctac
    22441 ttggtcaatt ccttcaaaaa gctggataac acggctagat tgtacaaaat aatccctata
    22501 accaagagag tcgagaaccg ttactgcttg ttccgtcgcc gaagtgtcaa tttttataaa
    22561 ttgacgagat tcttgtgcca attcttcttt cgataattct ttgatcaagt gtccttcatg
    22621 caaaatacca tagtgagtgg cgatctgtga caattcatct agcaagtggc tagaaagcaa
    22681 gacggtgatg ccccgttctt ttaccaaacg ttggatgatt tctcgcattt ctgccatccc
    22741 tgctggatcc atcccattcg tcggttcatc caaaatcaaa aattctggat tcgtgatcag
    22801 tgcatttgca atagctaaac gctggcgcat acctaaagag aaattctttg ctttcttttt
    22861 ccctgtattt tccaatcgca tcattttcag caggtcttcg atttctctat ccgacacacc
    22921 gccgttcgct gcttggatac gtaggttatc tcttgcagtt aattctggat aaagagcagg
    22981 ggtttcaatc gattgaccca tacgtcgtct tgctgcctgc aaatccgtcg ttccaaataa
    23041 ttcgatcgaa ccttcatcaa tcgagatcaa acccataata gttctcataa aggtagactt
    23101 acccgctcca ttttccccaa tcaatccata gatcattcca cgtttgattt cgattgatac
    23161 tttatctaat gctttggcag caccgtattt tttggttata ccagttgctt tcaagaccgt
    23221 ttcatttcca aaatccatac ttatccttct ttcttattga tgagtgaagt atagttgacg
    23281 ggtataaaga aagcagccag aaatgtataa agaaagtgta aattttccaa ttgaatgtca
    23341 aaaaaaacaa tcggacaata cgtgtctttc ctattatcag gcagtttgtt atacgcttgt
    23401 atcagtttcc tgattctttc ttttcgacca aagtcgtttc tctgtattcc tcttcactga
    23461 aaatttttgc ttctttcaga tcaagcttga gtaattttac cagaccggtt ataaggataa
    23521 ctatagcaag actccctccc agaaaaatta gactgtctcg cagacgtggt tgcgttgtct
    23581 tccaatcaaa gagcgcacca aacagtgcta atccaagtgg actggccacc atggtaatcg
    23641 ttgttatcgt tgacataagt cgtccttgta tatgcgttgg aacttctcgc tgaagccaga
    23701 ccatataaaa agcaaaagaa aacattaatc cttaaaaacg gacttatgaa aaataataca
    23761 tgccgtttgg gatttacaat tagctaccaa ataagaggtt atgacaaaag acttttcaca
    23821 atcccttctt ccgaataaac ggtgttctcc tgccaactcc tcggtcacaa gtcacaatat
    23881 ttatcaaaca tgagaagcaa tttttgtaaa ttccttctta tttcttgaag ctgaccactt
    23941 ctgtcacaac ctcagaagcg gataaataat attgtttgat gatactttat ctttttcccg
    24001 tttgtttata gatcgaatga tattatagac caagaatcct acctattaac atagctaaaa
    24061 acacaaaaaa ggcactttcc aaattgtgga aagtgccaga aaagttagga ttaaggtaac
    24121 tgcgctcagc attgcttcgc ccagtacgat tctcgtaact cgtgaactcg aacgataatc
    24181 gcaattaacg ttttgagaac tgtgaagctt tacgggcttt cttaagacct ggttttttct
    24241 tcgtatcatt gaataagttt gaaaaacctt gatttataaa ggttttcccg taaatatatt
    24301 ttattatatt tgattaaatt tgaaatgttt gtgttctaaa atgtgttcta atttaaaggc
    24361 agtatatttc gtattttaga ttaaattttt aggctcattc attctcaaat ccattctatt
    24421 cagtcaataa aaattttaaa aagactttat aaacaaagat gttcaagcga gcatcttatt
    24481 tttttgtgct taatttgaaa ggagaacatt tcatgtataa aaaacaaaaa aagaaattat
    24541 gtgtattaac tattttgagt ttggctgttc aagcaattgt tggagcaatt gctcctacga
    24601 ttacttttgc tgatgaaatt acccatccac aaacggtaac cgtagaacta gatttagcac
    24661 atcaatatgc tgttgaagga acttttagtg atggtcgtcc aatgtcagaa gtcactgttc
    24721 cgcattatgc agtgtacaac ggctttaaac aagatatttt ctgtattgaa ccaggagtcc
    24781 cgatttacaa tgagttcact cctggctatg agaaaaaccc gttaccagat atgtccgata
    24841 aagccaaatt agtttccgtt ttatggaaaa aagtaggtac agatgcagat actcatattg
    24901 tggcacagaa gatgatctgg caagaagtta atgggtatac ccttcactca ataaaacgat
    24961 cagatggtag tgccgtaaat atcgcagcca ttgaggcaaa aataaatcaa gccattgacg
    25021 attatcagaa gaaaccaacc ttccataata gtacggctaa aacagtatta gggcaatcga
    25081 ctactgtgac ggatacgaat aatctgaatt tgtcagagtt cgatgaggtt gtggaaaatg
    25141 ccgcaaacat tgattatcgt gtgaatggta atcagttgat aatcacccca aatgccaatt
    25201 caaaagaaag tggtgtgctg acgcttaaga aatctgctgg taccggaaca cctgtcgcct
    25261 ataaaaaggc tggccaacag actttaatgg ccggggcgat tgataaacct aatacctaca
    25321 caatcaaaat tgatgtggaa acggagggtt ctttaaagat caaaaaagtc gataaagaat
    25381 cgggtgatgt tgtaccagga acggttttcc atttagactt tggaaaaact ttacctgcaa
    25441 aagacgtgac tactgacaaa gaaggcattg ctacattgga tgagattcct catggtacaa
    25501 aagtaaccat taccgaaaaa tcagtaccgg cgccttatac gattgatacc acccccatga
    25561 ctaccaccat taaagcaggc gaaaccattt acgtgacttc aaaaaacgca cgggaaaaag
    25621 ggcaaatcat tctggataaa agcggagttg aaacagggag tgatctttgg aatgacaatt
    25681 attccttagc tggtaatacg tttgccattc gcaaagacag tccaaccggt gaaatcgttc
    25741 aagaaatgac cacggatgaa aacggtcatg cggaaacacc aaaagagatt gctaatgcgt
    25801 tggaattggg aacctattat gtgaccgaaa ctaaagctag tcatgggttc gtgaatacgt
    25861 tcaaacctgt aaaagtcgaa ttgaagtatg ccaatcaaac cgtagcccct gttaccagta
    25921 acgtaaaagg gcaaaatcaa gaagtcactg gcgaaaccac tttgacgaaa gaagacaaag
    25981 atactgggga taaagcacaa ggtaaggcag tctttgaggg tgctgaatac accttgttta
    26041 ctgctaagga tggaaaagct gttaaatgga gtgaggcttt taaaccagag ttagcgaagg
    26101 gaacgaaagg ttctgatgaa acggtcactt tgactttaga cgaaaagaac caagttgccg
    26161 ttaaacattt agctatcaac gagtattatt ggcaagaaac aaaagcgccc gaaggatata
    26221 gccttgatga aacaaagtat cctgtatcca tcaaaaaggt ggatgatagc gaaaaaaatg
    26281 ccgtgattac ccgaaatgtt acggcaaaag aacaagtgat tcgctttggc tttgatttct
    26341 ttaaatttgc tggatcagct gcgggcacta ccgaaactgg cttcaatgat ctgaccttta
    26401 aagtgtcgcc attggaaggg accaatgaaa tcacaggtgc cgaagatgaa gcgaccacag
    26461 cttataacga gcaattaggg tttgatggtt atggcaagtt tgaaaatctt ccttatgggg
    26521 attatttact tgaagaagta gaagccccag aaggttttca aaagattacg ccattagaaa
    26581 tccgttctac ttttaaggaa aacaaagaag actttgtgaa gagtgagtat gtctttacca
    26641 ttaccgaaca agaccaaaaa caaccaatta aaacggtgac cgttccttac gagaagctga
    26701 cgaacaaagc attttctgtt agtttgaacc gtttaatgct ctatgatttg cctgaggaag
    26761 aagatagttt aacttctctt gcgacttgga aagacggaaa caaagaatta acttctctgg
    26821 attctaccga gctagttgat aaattgagtt acaatctaca tgaaatcaaa gaagactggt
    26881 atgtggtagc gcaagccatt gatgtggatg cgacaaaagc tgcccaagaa aaagatgaaa
    26941 aagccaaacc agtggtgatt gcagaaacaa gcgcaacgtt ggcgaacaaa gagaaaacag
    27001 gaacttggaa aattcagcat aaattaaccg ccgaacaagt tttaaataaa actattgtct
    27061 tattcaacta tgtgtatgaa aataaggaag cttttgaagc aggcgataag ccagtagcga
    27121 aggatgttag cttgaataac caagcccaaa ctgtcagttg tacagtggaa caccatgttt
    27181 ctatccaaac aaaggcccac ctagaaaacg gttctcagac ctttactcat ggtgatgtgg
    27241 tggatatgtt tgatgatgtg tcgattactc atgatgtact ggatggctca aaagaagctt
    27301 tcgaaacaat tctgtatgct ttattaccgg atggtacaaa caaagaaatt tggaaatctg
    27361 gcaagattga ttatgaagta aatgataaag aattcaccaa aactgtgctt gcgaaaaaag
    27421 tagataccgg aaaatatcca gaaggaacca agtttacttt tgcggaaatc aattacgata
    27481 aagatgggaa tatcaatgga aaacacaatg aagatttgaa agaaaaatct caaaccttaa
    27541 caccaaaaga agttccaacc atactaagta cgccgaaaca accggaaaca ccaactgttc
    27601 caagtggctc tcaagaatct agtcccacag tgaagacctt ccctcaaacc ggggagaaaa
    27661 attccaatgt tttactgttc attggtttta ctttgatttt tgcgacggcg ggctattatt
    27721 tctggaatcg ccggaactaa ggtgatgaga tgatacaagt aaatcctaag aaccaacgtg
    27781 cgcgaccgcc tcctaaaaaa gtagtcgcgc tatttttgta tccaaaacta acaatgaaaa
    27841 gaggaaaaga agatggaatt gaaatttgtc gtgccagata tggctgaaac ctttggaaag
    27901 ttaagttatg ccggtgaagg agaaattata acagaaggat atggacggaa tactacggtg
    27961 attggtcgca gttatcattt gtattccagt aagcaacggg ccgatgatat tgaggtggta
    28021 gtagctgcgg aagccggtga aaaggatttt gatcaagacc aaccgctaaa agccgtgaat
    28081 ccccatttgg ttgccaaagg ctatgagatt gagaatcgtg ggtttactga ttacgtgttg
    28141 tatgttgatg atttagtgaa agcataggag gaaaagaaga tgagattaac agaaggtatt
    28201 gtcgtagatt caggtttaac atttgggaag ttgcgttttt ccgcattacg ccgagaagta
    28261 cggaagcaaa atgaggatgg aacaattggt aatgaggtaa aagaacgaac ctataattta
    28321 aaatcctctg cacaagggcg aatgattcaa gttagtatcc cagctaatgt acccttacgc
    28381 gagtttgctt atgatgcaga ggtagagttg gtcaatccaa tcgtggatac cgttgccaat
    28441 tatgtttttc gagaaggaac taccgttaat tggttcatta aggcggatga tttagtcttg
    28501 aagcggcagc cgaatcaagg aaatcctacg aaccaaaatg aaagaaagaa ataggtgaag
    28561 attatggaaa ttaatgttga acatatttta gattgtctcg atcaatatgg aaaaggcgag
    28621 ctgactgagg aacaactcgt taaagcatta acctctgatg aaaaaatgtt tctgattata
    28681 catcaaggtt tacttggaga aaatagcgac agtgaagaag actctgatgt actagactgg
    28741 ttaggagaag aagagtcttt ctttatagtt gtcgaagtaa atgaaaactt atgtcggcag
    28801 gcagaatcca ttttggaaga aattggtgtg gagatgccgg atgctattga aggcttttta
    28861 aatcaattgg ttgaaacgaa acaactcccc tcctcgtagc agtaaatgac tagcataact
    28921 ttttgaaatt gcctaatgaa atcctattgg gctttttcga ttggaggtgg aaaccatcaa
    28981 aatgtacaaa ggacatcgca tacgcgcagg agatcagcac ttggtttatc actttgttct
    29041 tggctggcta ctcgctctgt tcatcggttg gatgagcgtc ttttattttc aagagtttag
    29101 acaatttgat atttctaagc tatcactttc tactattgaa atcgtttggt ctataaaaga
    29161 tttagtatgt ctgcttggaa gtctcgcttt ttcaggagct atgatattgc tttatattca
    29221 cttttttctt gatcattggc gaagcttgtg gcatcgacaa aagctagctc ggatgattct
    29281 agagaaccat tggtatgaag tgaaacaaac tcaaagtgaa ggctttttca aagatttgaa
    29341 tagtagccga accagagaga ctatcagtta cttccccaaa atctgttatc gaatgaaaga
    29401 tggattactg tctattcgcg ttcaaatttc acttgggaaa taccaagatc agcttttaaa
    29461 attggaaaag aagttagaaa gcggactgta ttgtgagtta gtagaaaagg aactcaaaga
    29521 ctcctatgtg gagtatactt tactgtatga tatgatcgcc aatcgaattg ggatagacga
    29581 agtagtggca gaaaatggga ctctgcgatt gatgaaaaat caagtatggg cttatgattc
    29641 tttaccccat atgttaatcg ctggtggcac gggtggaggg aagacctatt tcctcctcac
    29701 cattatcgaa gcactattga agtcagatgc agaattgttt atcctcgatc ccaaaaatgc
    29761 agatttagct gatttaggta cggtgatgcc tcatgtttat tcacaaaagg aagaaatttc
    29821 tgcttgtgtg gaagatttct atgaacgtat gatggctcgt agcaaggcaa tgaaagaaat
    29881 gtctaactac aaaacaggag agaattatgc ctatcttgga cttccaccca actttttaat
    29941 ctttgatgaa tacgtggctt atatggagat gttaacgacg aaagaaagtg cggtgatttt
    30001 gaataagctc aaacaaatcg tgatgttggg tcgtcaatct ggtttctttc tgattttggc
    30061 ttgccaaagg ccggatgcga aatatttagg agacggaatc cgcgatcaat tcaactttcg
    30121 cgtggctttg ggtcgtatga gtgaactcgg atattccatg atgtttgggg aggtcgaaaa
    30181 aaacttcttt atgaagcaca tcaaaggtcg aggttatgtg gatacaggtg gcagtgtgat
    30241 tagtgagttt tatacgccac ttgtaccaaa agggtatgat ttcttagatt caattagtaa
    30301 attagaaaag atgaatgatt gagtgatctt caatactgac tttatacttc aagtaacgta
    30361 taatactttt atatagtagg aagaaagggg ataattaata tggaaagtaa tgattcttat
    30421 ttagagtatg caaaagcaaa taaggataag tttatttctt atattatcaa agggaaatcc
    30481 cccgaagttg gacgagacgc agtttttatg gcagggagtc ctggggctgg aaaatcagag
    30541 gttgccattg gattagctga gaattatgat aaccatgtga ttattgatgc ggattacttt
    30601 cgcacccagt ttcctgatta taatggaaaa aactctagtc tctttcaaaa agcatcttct
    30661 tggttggtag agcaggcatt tagatatgtc ttagatcatg gctattcttt tattctggat
    30721 gcaacttttg caatattaag tgctgaaaaa aatatctcgc gggcgttgaa aaaacatttt
    30781 cgagtgacga ttttctatgt ttatcaagac cctaaaatcg cttggaaatt tacacaagaa
    30841 cgtgaaattg cagagggacg tcatgtgccg aaaaaggcat ttattaacgc ttttttcaaa
    30901 tcaagagaga acattgcaaa agtaaagagt aaacatccag atgtattgct tcatatcata
    30961 ataaaagatt atacgaataa tatttccgaa gttcattatg ctacggataa tctagaatta
    31021 tttttaccac tagggtatag taggaaagat ttggaggttc aattacatga ctgagaaaga
    31081 acaagtaacg aaaattgtaa agaaatacaa taagagcatt gcggatttat cggaaaatgc
    31141 tactgcaaaa gagttcaaaa cggtgattaa atatgtagcc gatcaggcca atgaaaaaca
    31201 acggaaatta gttggattaa ataaaaaata gtaggacaaa ttaataatgt atgagccaag
    31261 agcaaaatgt tcttggcttt tttctttgca atgaatgagg taatgtatga gatttttaga
    31321 tttattcgcc ggtattggtg gctttcgttt aggtatggaa caggcgagcc atcattgtat
    31381 cggtttttgt gaaattgata aatttgctag acgtagttat aaagcaattc atgatacaag
    31441 taaggaggtg gaaatgcatg acatcacaag tgtatcagat gagtttattc aatccctcgg
    31501 accagtggac atcctttgcg gcggatttcc gtgccaagct ttttcaattg cgggaaagcg
    31561 gcaaagattt tccgatactc gaggtacttt attctttgaa atcgctcggt tcaccactct
    31621 tctccgacct aagcttttat tccttgagaa cgtccgagga ttgctcaatc acgaaggagg
    31681 ggctacgttc gagacgatcc tccgaacgct ggatggattg gggtatgacg tggagtggta
    31741 ggtgcttaac tcaaaggcct acattcccca aagccgggag cggatattcc ttatcggaca
    31801 ttctcgagac gcatgtaccg aacaagtatt tcctatcatt ggaccgtctt caacatctga
    31861 tcaaaacatc agaaatttgg taaatatcaa tccttccaat cgaggaatgg gtggacaagt
    31921 ctatggttca gatggcgtag cgccaacctt aacaggtgat gaaggaatca aaatcgcact
    31981 gccagcaagg gatggaatct ctgtagcagg actattaccg ggaaagtttg aacaaggtaa
    32041 tcgagtttat gaagttactg gaaccgcccc caccttgtca acgaaacaag gtggtacaaa
    32101 aatcatgatt cgaaaaaatg aaacagaata tcaagaggtg aagccagaag acagtgtgaa
    32161 tctggctttt cctaattcca caagtcgccg aggtcgtctg ggaaaacaga gtgttcatac
    32221 cttactaaca ggagaccaac aagcgatcgt cacggatcag tatcaaattc ggaagctaac
    32281 acctagagag tgttggcgac ttcagggctt tcccgattgg gcttttgatc gagattctca
    32341 agtcaattca gatagtcagt tgtataagca agccggaaac tctgtaacag ttccggtcat
    32401 ttttgatatt gccagaagat taaaagagtt ggatgtacat ggtttatgat gagttgattg
    32461 gagaaattta ttgggtgatt ggaaagattc agtcagaccc agaattagaa gaacaattgc
    32521 gacggttaaa atttgatatt cgcaagaatg gtgtcaaagt cgctggcgat ccatatttaa
    32581 tgaatgagga gacggatgct cgaattgaaa taaatcaagt aattgcagaa tttgagcgga
    32641 ttgcggacca agcaaaagaa ccagatattc gtcaatactt gttagaaatg aaagcagagt
    32701 tggaagtaaa tggtattaca gatgagtaac ctaaaccaac acaactgact gttaggcgct
    32761 gaccgcggag gattcgcgaa gcagaaatcc tccgtggtgc tgtgcctgtt catggacggc
    32821 gttagccgac catgatgtct cacccccgta tctaacaggg gggtacaaaa acacaaaaaa
    32881 taagtaagca accattaaga atgcttgttg tatcaaagat agtaagtaat ttgtataggt
    32941 gttaactcat ttgaaaaagt taacaccttg aatttggagg tgagaacttg gcacaacgga
    33001 atttagatta tcggctatta aaagaccgac gcaacgaata cggcgtttca caaaataaat
    33061 tggctacggc ttgtggactt agtcg
//
LOCUS       DARBET010000026        32178 bp    DNA     linear   BCT 07-NOV-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4027
            SAMEA114569841-rid20112183.denovo.026, whole genome shotgun
            sequence.
ACCESSION   DARBET010000026 DARBET010000000
VERSION     DARBET010000026.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA114569841
            Sequence Read Archive: ERR12203666
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 32178)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 32178)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001972618.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001972618.1
            Genome Coverage       :: 132x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/07/2023 13:18:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,041
            CDSs (total)                      :: 2,975
            Genes (coding)                    :: 2,908
            CDSs (with protein)               :: 2,908
            Genes (RNA)                       :: 66
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 67
            CDSs (without protein)            :: 67
            Pseudo Genes (ambiguous residues) :: 0 of 67
            Pseudo Genes (frameshifted)       :: 26 of 67
            Pseudo Genes (incomplete)         :: 28 of 67
            Pseudo Genes (internal stop)      :: 20 of 67
            Pseudo Genes (multiple problems)  :: 6 of 67
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..32178
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA114569841-rid20112183.denovo.026"
                     /isolate="VRE4027"
                     /db_xref="taxon:1352"
                     /country="Denmark"
     gene            complement(407..766)
                     /locus_tag="SCJ86_001329"
     CDS             complement(407..766)
                     /locus_tag="SCJ86_001329"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629779.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YccF domain-containing protein"
                     /protein_id="HEG1168901.1"
                     /translation="MSCLGNVIWFIFGGLIGGLSWLAAGILWCITIIGIPIGMQCFKI
                     ASLSFAPFGKEVIYHQSGMSLIVNIIWLIVSGLPIALAHVVSGILLCITIIGIPFAKQ
                     SFKLARLALMPFGAEIV"
     gene            complement(796..1137)
                     /locus_tag="SCJ86_001330"
     CDS             complement(796..1137)
                     /locus_tag="SCJ86_001330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338940.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEG1168902.1"
                     /translation="MMEIRWAVKEDLPQINQLITDHPESFVKAKTAADLNDKNKLLLV
                     AVTKEKRIIGFAQADVQQSEERVDQRPSGKLIQHTLVTKENTEEIQKKFAEYLMLEAK
                     KRRIKSIFAQF"
     gene            complement(1134..1808)
                     /locus_tag="SCJ86_001331"
     CDS             complement(1134..1808)
                     /locus_tag="SCJ86_001331"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288769.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SGNH/GDSL hydrolase family protein"
                     /protein_id="HEG1168903.1"
                     /translation="MKKRILFIGDSITDAGRDRAEATDLGKGYPLLIAGKLISQYPTM
                     KFECMNRGIGGDKIADLQVRWQEDCLALQPDYVSILIGINDTWHNTSDQAIFGTEKSA
                     QQFESVYRKLLNELQTAEICNIILMEPFVLPITDDRHGWRKDLDPKIQIVRKLAKEYG
                     ALLVPLDGIFNALGIAYGFSKYTREDGVHPTIGGHEIIAQSWIDAIAAVDFFSGDKNE
                     RKKSEK"
     gene            complement(2984..4282)
                     /gene="asnS"
                     /locus_tag="SCJ86_001332"
     CDS             complement(2984..4282)
                     /gene="asnS"
                     /locus_tag="SCJ86_001332"
                     /EC_number="6.1.1.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016180901.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="asparagine--tRNA ligase"
                     /protein_id="HEG1168904.1"
                     /translation="MEQIQIIDSKNHVGETVTIGAWVANKRSSGKIAFLQLRDGTAFF
                     QGVVVKSEVSEEVFQTAKNLHQETSVLVTGEIREDSRSKFGYEIGITNVEVVSESHDY
                     PITPKEHGTDFLMDHRHLWLRSSRQHAIMQIRNEIIRATYEFFNQHHFVKIDPPILTG
                     SAPEGTTDLFETNYFDQKAYLSQSGQLYMEAAAMAFGKVFSFGPTFRAEKSKTRRHLI
                     EFWMIEPEMAFTHQEESLEVQEQYVAFLVQNVLDHCEYALDVLGRDKEVLKTYTKLPF
                     PRITYDDAIELLKKNGFDDIEWGEDFGSPHETFIANSFEQPVFILNYPKAIKPFYMKP
                     HPTREDVVICADMIAPEGYGEIIGGSERATDYDFLLEQIRNHGLDEKEYSWYLDLRKY
                     GSVPHSGFGLGLERTVTWIAGIDHVREAIPFPRLLNRIYP"
     gene            complement(4322..5512)
                     /locus_tag="SCJ86_001333"
     CDS             complement(4322..5512)
                     /locus_tag="SCJ86_001333"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291769.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal phosphate-dependent aminotransferase"
                     /protein_id="HEG1168905.1"
                     /translation="MELSKRTKRLEPSVTLAAAAKAKALKAKGRDVLSLTVGEPDFAT
                     PENIQEAAIEAIRNGKASYYTPTAGIPELRQAIVDYLKKNYGLVYEPSQTVVTDGAKF
                     ALYTLFQTILDPQDEVIIPVPYWVSYGEQVKLAEGLPVFVKGEESNSWKVTVEQLEQA
                     RSEKTKAVIINSPSNPTGMIYSKNELQAIGEWAVKNDILIVADDIYGRLVYNGNEFTP
                     IATISEAIKNQTIIINGVSKTYAMTGWRIGYAVGNPVIINGMIAVASQSTSNPTAVSQ
                     YAAVEALKGEQDTVEEMRNAFEERLNTLYPLVAELPGVSLEKPQGAFYLFPNVKETLR
                     MCKYENVTQWVEDLLEETGVALVTGEGFGAPENVRMSYATDRATLEEAVRRIKQFIES
                     KSQN"
     gene            complement(5533..6060)
                     /locus_tag="SCJ86_001334"
     CDS             complement(5533..6060)
                     /locus_tag="SCJ86_001334"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002316397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5590 domain-containing protein"
                     /protein_id="HEG1168906.1"
                     /translation="MKIKERRPLKKKQTKEKTINRVLLGLIVFLLAVIVISSIFYLRS
                     SRPAVQAKKEAVEIAKKYANLEETDHFYWFTRKKTYFSLTGTNDKGKEIAVIIPKSGE
                     KVKVLDQTKGLTENEAKQTIAAAHPEIQIEKAALGMYDDQPVWEVVGKETNGKLNYYL
                     IGFESGDEVKTIKGI"
     gene            complement(6107..8896)
                     /locus_tag="SCJ86_001335"
     CDS             complement(6107..8896)
                     /locus_tag="SCJ86_001335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628443.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase family protein"
                     /protein_id="HEG1168907.1"
                     /translation="MKPRTYAVVDIETTGTNPKEDRIIQFGCVMIESGRITSRFSIDI
                     HPGRKISKQIQHLTGITNQRVQKAPYFEDVAQTIYNLLADTIFVAHNIYFDYNFLNHE
                     LMRCGLPSLKIPGIDTVELAQIFLPTEPSFRLADLSESLGFIHENPHQADSDAEVTGQ
                     LLLLIEERMRKLPIITLEQIARLSRHTGMDTSRFIYHVIEEMKEKPEPLDPSLEIVDG
                     LALQKKEVELFTSVHYGERTYPRKKQAKEKMFGKTLMYRKEQNRLMNAVYDHFTKDES
                     KDLMIEAATGMGKTIGYLLPLSYLATPEKPAVISTVSLVLQQQIIEKDIPLLNQLLDQ
                     PIQPVIIKSYRHYIDLQRFKGTLVEPPEQKQYALYQMAVLVWLTQTKTGDLDELHLTN
                     LNHSFFADIAHRGIGFLARNQSFYEQDFVRFLQKKIRQSNFLIVNHAFLVQETQRKEP
                     LLPASPFLLIDEVHHLPEIAEKVNGKRLSINYFHKQIQQLEEPNQLFDRIQSYLPKEH
                     EALRWLSIYREELSALCEAQICMAEGLMSLVSGKQPNGQYPEEVMMTKEMLDQLPLES
                     GTAIDHLQLFYQEIQQLQSQMRLTFEIIQESWTNQMRIDLASLYSLFDQLDKQYLLMD
                     QWLEDWRDHLIHWFVPGNTAQQMIFHLHDFQAAMLPSTVWYPRYDRILYIGGTLKIGP
                     NRHYLSKRLGIEAAPLKVIASPYDYEKQARIYVPKEAPAVHQVSNQAYIDYLETALTG
                     LIEQEDRPILVLFTSHDVLQKVYQKMHLSMLEKGREILAQGFGGSREKLLKRFILSEK
                     SVLFGADSFWEGVDLPGDALQIVVVTRLPFDNPLRPMVKARNAYLESEGINPFYQESV
                     PKAALKLRQALGRLIRTETDRGVLLILDRRLVTAKYGARIIHALPKKVPIKELAMEEI
                     YQDIHEFLKKNEEGS"
     gene            complement(9043..9243)
                     /locus_tag="SCJ86_001336"
     CDS             complement(9043..9243)
                     /locus_tag="SCJ86_001336"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003234247.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cold-shock protein"
                     /protein_id="HEG1168908.1"
                     /translation="METGTVKWFNSEKGFGFITAENGNDVFVHFSAIQGDGFKTLEEG
                     QAVTFDVEEGQRGPQATNVTKA"
     gene            complement(9696..10016)
                     /locus_tag="SCJ86_001337"
     CDS             complement(9696..10016)
                     /locus_tag="SCJ86_001337"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008268003.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEG1168909.1"
                     /translation="MKKRLLWCLLSLSLSLTSQPVFAEETQRFPITGTRLYKNVYVQT
                     PDLDSAIPETEERSLQPRFGYFWYCPTCDYESCWHFLHSTAAAFAFTHTQKYDGHHPV
                     IYEV"
     gene            complement(10519..11637)
                     /locus_tag="SCJ86_001338"
     CDS             complement(10519..11637)
                     /locus_tag="SCJ86_001338"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEG1168910.1"
                     /translation="MAKKDKELLSQLTSEGAKGPFVTIMLNTHVAHQDVEKDQIKFKN
                     FAKEAKKRFEKKYSEHDWSPFQEKIENLLGDPSFWRSATTSVAVIITADQTYIHRLSI
                     PVDDQYYVSDLPYLLAIIKNGQFNYSYYLLGLNRDSMKLYEVSNKQVKEVSLPEGAPV
                     DVVTALGSELTGGGLNFSSNGGGGARDGVTFHGVNPKDEEVEIDWVNYYQAVDTYLKD
                     SFENPEQWPIYLFALPENQTMFKKIAKNPYYCADVAISASPAQLSFRDIEEGAKKLSS
                     ELAAQESKEYNKLLDKKFIDQLVDIVPAAKEGKVSHLFIATSNLADGFGEDPETEYDR
                     RQVLNALADDVIINGGKVFILDQKDAPDEKSLTAILRY"
     gene            12108..13415
                     /locus_tag="SCJ86_001339"
     CDS             12108..13415
                     /locus_tag="SCJ86_001339"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302201.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanyl-D-alanine carboxypeptidase"
                     /protein_id="HEG1168911.1"
                     /translation="MSIKKTRLVLLLASLILCLASFLPAVAVQAEDTFKVNAKAAFAV
                     DAQSGKILYDQDGEKTMGIASITKIIGLYIVLDQVKEGKLSWDDKVSISDYAENLSVT
                     PDLSNVPLHKENTYTVKELFDSAIIQSANASMVALAEKISGSEAKFTERMKEQLKDWG
                     IKDATIVNASGLNNSYLGENRPEGTGENDENQMSAQDVAIVARHLILDFPEILDVSST
                     TTQMFGENTQSPVEMVNWNWMLPGFVNYKEGVDGLKTGTTELAGACFVGTITKNDQRI
                     ITVVLNADGHAENPSARFNETARLMDYCYDNWSEKELGKANASIPSLKTIDVKDGKES
                     TVPVVLKSPVKVWVRNDMDTGKLTITPTLDKKQVTDNEIQAPMDKGTKIGTATITLAE
                     DKLGYLEDNNAPSTEIITDKTVEKANIFVIGWRRVAEFFGNLF"
     gene            13536..14735
                     /locus_tag="SCJ86_001340"
     CDS             13536..14735
                     /locus_tag="SCJ86_001340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296299.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdcF family protein"
                     /protein_id="HEG1168912.1"
                     /translation="MNLTRDLIFLVLYLIGVFSIVYWRKQKKDPSLFFYSSTWTFAVI
                     LTYFVVLEIFYSTFYFLIPLVFFSIFAFSYFSEKRRLLNGLLFNIFLISFGIYLFVLL
                     YETQNIFLGGLIALITIPLLLVFLFGIYGLIVFLFWNGVTVLRRESHSLANLLTLILA
                     IFLTLFLVFDFFLLKYLPQWINALFFCIPLILIYLFIVFYNFLTVSFLYQFNRPRYNQ
                     DFIVVLGAGLINGETVSPLLAKRINKAIAFYWAQSRATLNPPILLMSGGQGADEKVPE
                     AMAMKQYAMEQGIPERDILVETNSTTTLENMRYSKEIMDQQMKGPYRAIFSSNNYHIF
                     RAGLYARQAKLKANGIGAKTAFYYLPNAFLREYIAILMMNKKRHMIICGLLFAGSAFL
                     SLITLLF"
     gene            complement(14762..16030)
                     /locus_tag="SCJ86_001341"
     CDS             complement(14762..16030)
                     /locus_tag="SCJ86_001341"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291753.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="voltage-gated chloride channel family protein"
                     /protein_id="HEG1168913.1"
                     /translation="MEKQLPSPVRILMYMLKWLVVTAILGVFMGSLSAFFLNSLTFVT
                     DIRLAHPWLLYLLPVSGALFAYLYAYHGGLSSRGNNLVIDQGNGGEEKIPLRLIPLTL
                     FGTITTHLFGGSVGREGTAVQMGGALADNIAHLFRLDKAEREILVISGISAGFSSVFG
                     TPLAGTLFGLEVLAIGKVRTEAIFPSFFAALFANFMTESYGVSHLHYQMGVIPEWSML
                     LFFKVFVAGIAFGLIGWVFSRSIVFLKARYAQWIPHPVWRNTIGGAVVVAAALILQTQ
                     RYLGLSLPLLQESFNGQAHAYDFIGKLFFTVLSLGAGYQGGEVTPLFEIGATLGAVLA
                     PLLHVSVPFLAGLGFIGVFSGATNTPIACFVMGIELFGSQAAAYFFMICLISFMCSGN
                     SGIYSAQQVAVKKGILFLPIVKRIKEKNKV"
     gene            complement(16200..16841)
                     /locus_tag="SCJ86_001342"
     CDS             complement(16200..16841)
                     /locus_tag="SCJ86_001342"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010728800.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor G-binding protein"
                     /protein_id="HEG1168914.1"
                     /translation="MEQTMKPYQYFFIKREVEQLLNAYSSVNDSKTVQTVQALAAEKI
                     RDVLGYEPSEIDTFLTTVLDVKLTKTQAERALEELKQIVQPFMQPSKPQIEKLFRKVK
                     KLKQPAWNEIDLQAHTYIGWNDPGTQKKFMVYYEDGKLKGISGTLSPTINKGICAICQ
                     KTSNVSLFLSTTKAGSDGTYTKKGNYICHDSDQCNQQLTKLAPFYEFAERVKK"
     gene            complement(17250..17753)
                     /locus_tag="SCJ86_001343"
     CDS             complement(17250..17753)
                     /locus_tag="SCJ86_001343"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348853.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing protein"
                     /protein_id="HEG1168915.1"
                     /translation="MTEKWREDEEYMSYVEDLLETEAVKKLANYTQHVHSTRLEHSIS
                     VSYYSYLLAKKWGGNAKATARAGLLHDLFYYDWRTTKFDEGTHAYIHPRIAVKNAEKI
                     TDLSDLERDIILKHMWGATIAPPKYKEGYIVTFVDKYCAVKEAAQPMSASMRKRWQRY
                     FGKESSI"
     gene            complement(17876..18640)
                     /locus_tag="SCJ86_001344"
     CDS             complement(17876..18640)
                     /locus_tag="SCJ86_001344"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334496.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA methyltransferase"
                     /protein_id="HEG1168916.1"
                     /translation="MKEIQSAKNPRIKELKKLHKKKYREEKEEYLLEGFHLIEEAASS
                     DAEIKELFVTQRGMKEWGDWLAANKEISTFLVSDEVMDAVSDLPTPQGILAVMALTAE
                     GTPDFSGGWLLLDNVQDPGNVGTMIRTADAFDMAGVLLGKGTADIYSTKVLRSMQGSN
                     YHLPVLQKDLTEVIPLMKDKGFTVYGTELNEEAHALDKVEKSDNFAIIMGNEGQGVSQ
                     EILSLTDQNVYIPMKGNAESLNVGVAAGIVLYHFSL"
     gene            18748..19023
                     /locus_tag="SCJ86_001345"
     CDS             18748..19023
                     /locus_tag="SCJ86_001345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346033.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acylphosphatase"
                     /protein_id="HEG1168917.1"
                     /translation="MRKVKMNVQGRVQGVGFRYMTKMVADQLGVTGTVKNEDDGSVTI
                     EAIGNDDIIQKFIEEVKKSPSPSGRVQYVDVQEDPMIEERKKFDVVG"
     gene            19583..20533
                     /gene="yidC"
                     /locus_tag="SCJ86_001346"
     CDS             19583..20533
                     /gene="yidC"
                     /locus_tag="SCJ86_001346"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306243.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane protein insertase YidC"
                     /protein_id="HEG1168918.1"
                     /translation="MNKWKNWLLGSGLVSILLFLSGCVRMDSNGNPDTSGIVYRVLVH
                     PMGQAITYLVENFNWSYGWAVIAMTVIVRIIILPLGISQSKKTMIQSEKMQALKPQVE
                     AAQQKLKAATTREEQMAAQAEMQQVYRENGLSMTGGIGCLPLLIQMPIFSALYFTARY
                     TEGIRESSFYGIDLGSPSMVLVVIAGVAYLLQGYISTIGIPEEQKKTMRTMLIVSPAM
                     IVFMSISAPAGVTLYWVVGGVFSCLQTFITNVMMKPRLKAQVAEELKQNPPKQVVTPR
                     KDVTESAKPANNKQATKQIKQPKNSTNNKGRNAGKQQKRK"
     gene            complement(20579..20980)
                     /locus_tag="SCJ86_001347"
     CDS             complement(20579..20980)
                     /locus_tag="SCJ86_001347"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014387104.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS fructose transporter subunit IIA"
                     /protein_id="HEG1168919.1"
                     /translation="MSRQLVLISHGKFCEELKKSTEMIMGPQEDIYTLPLLPEDGTET
                     YKQKFLTVTDSLNDFVVLCDLLGGTPANIISKLIMEGQQITLYAGMNLPMVIEFINSQ
                     MIGSEPEFVRAGCESIVSVNELINGIAEEEE"
     gene            complement(20994..21803)
                     /locus_tag="SCJ86_001348"
     CDS             complement(20994..21803)
                     /locus_tag="SCJ86_001348"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311319.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS system mannose/fructose/sorbose family
                     transporter subunit IID"
                     /protein_id="HEG1168920.1"
                     /translation="MTKSNYKLTKEDFKQINRRSLFTFQLGWNYERMQGSGYLYTILP
                     QLRKIYGDNTPELKEMMKTHTQFFNTSNFFNTIITGIDLAMEEKEGIASKETVSGLKA
                     GLMGSFAAIGDSVFAALIPTIFGALAANMATDGNPIGVFIWIFAQIVVMVFRWKQLEF
                     AYKEGISLVTTMQSRLTALTDAATLLGVFMVGALVATMINVKFSWAPTIGDVTLNMQN
                     YIDMILPKILPAGIVGGVYWLLGKKNMTPTRAIFIVLIVCVALSALGVISK"
     gene            complement(21790..22680)
                     /locus_tag="SCJ86_001349"
     CDS             complement(21790..22680)
                     /locus_tag="SCJ86_001349"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002364095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HEG1168921.1"
                     /translation="MDIQWWQILLLTLYAGYQILDELQIYSSMSAPVFAGLYAGLVMG
                     DLNAGLIIGGSMQLTVLGVGTFGGASKIDANSGTILATAFSISLGMNPEQAIAAIAVP
                     VASLMIQLDILARFANTYFAHRIDRHVEDMNYQAIERNFLMGALPWSLSRMIPVFLAL
                     AFGGGLVENVVSVLNGNLKWLGDGLSVAGAVLPAVGFAILLRYLPVKKHLSYLILGFT
                     ITALLATLFSNVQLLGTSVASVVKDLPATFNSLPMLAVALIGLALAMISYKNDQNKDH
                     QPLASEPSHSVEGEIEDDEI"
     gene            complement(22693..23172)
                     /locus_tag="SCJ86_001350"
                     /pseudo
     CDS             complement(22693..23172)
                     /locus_tag="SCJ86_001350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016632653.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="PTS system
                     mannose/fructose/N-acetylgalactosamine-transporter subunit
                     IIB"
     gene            complement(23348..24289)
                     /locus_tag="SCJ86_001351"
     CDS             complement(23348..24289)
                     /locus_tag="SCJ86_001351"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346036.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hexose kinase"
                     /protein_id="HEG1168922.1"
                     /translation="MITTVTLNPSIDIAYTIEELNLDKVNRVETVNKTAGGKGVNVAR
                     VLNLLGEEVIATGLIGGQHGEYLKNQLDQLGIMHQFVPIKGETRDSIAILHSSRQTEL
                     LERGPFITKKEQQTFTDHFEQLLEISDIMTISGSLPQGCPLDYYKQLVALADKKGKDI
                     LLDTSGKFLLEVLEGKEKPLLIKPNLEELGELIGQRICSSIQLEKIQSLLAHPIFEGV
                     PWVVVSLGERGAVAKVAQCFYEVIIPKISVVNPVGSGDATLAGSAHALSKRQSIEEIL
                     AFSMACGMANAQQEKTGWIDPMKVTMLMKHIQVKKIK"
     gene            complement(24286..25281)
                     /gene="lacD"
                     /locus_tag="SCJ86_001352"
     CDS             complement(24286..25281)
                     /gene="lacD"
                     /locus_tag="SCJ86_001352"
                     /EC_number="4.1.2.40"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346037.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tagatose-bisphosphate aldolase"
                     /protein_id="HEG1168923.1"
                     /translation="MNEEKNRAMERLSTKDGIISALAIDQRGALKKMIKSLGKEPSED
                     QISEFKELVSAELTPYASAILLDPEYGLPAAKVRAKETGLLLAYEKTGYDATTPGRLP
                     DLLADWSSLRLKEQQADAVKFLLYYDVDEAPEINHLKHVFIERLGSECEGENRPFYLE
                     LVSYDAQISDSSSPEYAKVKPHKVIEMMKEFSDPKYKVDVLKVEVPVNMNYVNGYSTG
                     EFIHSKEEAAAFFKEQSEATPLPFIFLSGGVSTELFQQTLIFAHESGSTFNGVLCGRA
                     TWKDGVKAYVENGKDGAIHWLRTNGRENIETLNKVINETATPWFNKTKSKKVKMK"
     gene            complement(25274..26470)
                     /locus_tag="SCJ86_001353"
     CDS             complement(25274..26470)
                     /locus_tag="SCJ86_001353"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875503.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SIS domain-containing protein"
                     /protein_id="HEG1168924.1"
                     /translation="MFELSKNKLKQIGAAITTQEIKQQPDLWKETIDRLMQSQENLAL
                     FFDQIKKEANGRRIRVIFTGAGTSQYIGDTLLPYLNEHGDNQFFIFHSFGTTDIVASP
                     KEFLFPNEPTLLVSFARSGNSPESVAAVQIANEQVETLFHLTITCAKEGMLAKNAQDD
                     KQNYLLLMPERANDAGFAMTGSFTCMLLSALWIFDQTAMEKKERYLEQLVKIGQDLLS
                     RDQEIQKLLPDFFERIAYLGSGSLAGATREAQLKILELTAGKVATIFDSSMGFRHGPK
                     SFLNSETLVVGFVSNQPYTRNYDLDILEEIKEDRVTANVIAIAQPGTIDFSGMTVLLE
                     DSVSKLPDAYLALAMIMTAQIIALYTSIKVANKPDTPSPTGTVNRVVKGVIIHKYETE
                     GEMTDE"
     gene            complement(26488..27216)
                     /locus_tag="SCJ86_001354"
     CDS             complement(26488..27216)
                     /locus_tag="SCJ86_001354"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177465.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HEG1168925.1"
                     /translation="MESSNKGKSLYYQLVDLLKDRIETQMTPHDKLLSERELCQHYGV
                     SRTTVRLALQELENQGYIYRRHGKGTYVSDLSDKSSNLAGAYSFTEEMREIGRVPKTV
                     ILEFRQMEANKRIAKNLNIHLGETVFKLKRLRLADEEPLMVERSYLPAKLFMGLDLPL
                     LESKPLYDVFSQDYEQQVQLADEEFYASIVQKKDAVVLRIADGAPVLHLTRITYNVKN
                     EIIEYTDSVARADQFHYKIRHIRK"
     gene            complement(27657..28631)
                     /gene="lacD"
                     /locus_tag="SCJ86_001355"
     CDS             complement(27657..28631)
                     /gene="lacD"
                     /locus_tag="SCJ86_001355"
                     /EC_number="4.1.2.40"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002308674.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tagatose-bisphosphate aldolase"
                     /protein_id="HEG1168926.1"
                     /translation="MKKMSIEKQERLKRLTDEAGIISALAIDQRGALKKMINQYKEAT
                     SEDIIQFKQMVSQYLTPYASAILLDPEYGLPAAALREKECGLLLAYEKTGYDAKMPGR
                     LPDLLNSWSVKRLKEVGADACKFLLYYDVDEPVEINVQKQIVIERIGSECEAEGIPFF
                     LELLSYDATIGDPSSLDYAKVKPHKVIEAMREFSKERYQVDVLKVEVPVNMRFVEGYG
                     QDTCYSQAEASAYFYEQSKVTEQPFIFLSAGVSAALFQDTLRFAKQSGSTFNGVLCGR
                     ATWAKGVAPFVTEGEEKASSWMLEEGKQNIKELSTLLEETATSIYVNK"
     gene            complement(28628..29086)
                     /locus_tag="SCJ86_001356"
     CDS             complement(28628..29086)
                     /locus_tag="SCJ86_001356"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294893.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HEG1168927.1"
                     /translation="MTIIHEENIFLQQPLDNQEEAFKFLAKQTFCLGIAHDEKEVYQK
                     LLEREKEGTTGIMSGFAIPHAKSQTINEPSLIIVTLKKGIEWNSLDRQSVDFIFALFI
                     PDSEAGTTHLKLLSLVARSLMRKEVTEQLRAAKSKIEIATILTKEIGEDI"
     gene            complement(29083..30546)
                     /locus_tag="SCJ86_001357"
     CDS             complement(29083..30546)
                     /locus_tag="SCJ86_001357"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002308672.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HEG1168928.1"
                     /translation="MGKYQLIAATGCPTGIAHTYMAQEALEQAARKKGITIKVETHGQ
                     IGIENELTAEEIKQADAVIIAADKDVHSERFVGKRVIDVSVSTGIKDAQRLIDEALAG
                     KGTVLADNEAADTLEPEAISGANFGRSIYKNLMNGVSHMLPFVVAGGVLIAISFAVWG
                     IYSSDPDHAQYNATAAMVKAVDDAAMGMMVPVLAAYIAEGIAKRPGLVVGFVGGIVAM
                     NGGTGFLGGILAGFLAGYFILFLQKLLIILPKQLDGLKAIVLYPVIGVAFIGIVMSLL
                     AGPMKAINEGMMSFLAGFEYSNPLVLGVIVGCMCAFDMGGPVNKAAYVTGTALLTQGN
                     TTFMAGASAACIAPPLITGFATLLFGKYFDTNDRNAGLVNFILGSTHITEGAIPFAAK
                     DPIRVLPTMMLGSSIAAVLTYLFGVQVPAPHGGFLVLPVVTGKIQWVTAILIGSIVGG
                     VILGLIQKKRVTDKDKKRDNKATNRTVIEEGRAGGLS"
     gene            complement(30528..31460)
                     /gene="pfkB"
                     /locus_tag="SCJ86_001358"
     CDS             complement(30528..31460)
                     /gene="pfkB"
                     /locus_tag="SCJ86_001358"
                     /EC_number="2.7.1.56"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1-phosphofructokinase"
                     /protein_id="HEG1168929.1"
                     /translation="MSIYTCTMNLAIDLFIETDEMHPFMVNRTKEDDIQANGKGVNVS
                     LVLKMLDFPSTALGFSAGFTGKYIEDYLQQKQIQTEFIEVPGMTRINVFTQVNQTGEE
                     YKLVNQGPEIPQTAVHNFLNQIRALQAGDYLCVSGSLPRGLSPKVLIEISRICLIIDS
                     SDQEVMDCLPYRPFLLKPNEEELSSWFGRKMVTDEDYFVYGQRLVESGAENVLLSLGE
                     KGAILFTKDRVIRGNSPKGKVVNTACAGDAMLGTFLAGYMNRRSLDETLRKSIAAGSS
                     TAFRKGLTDFLDVEELSKQIKIKEERFERWENIN"
     gene            31602..>32178
                     /locus_tag="SCJ86_001359"
     CDS             31602..>32178
                     /locus_tag="SCJ86_001359"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738096.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MurR/RpiR family transcriptional regulator"
                     /protein_id="HEG1168930.1"
                     /translation="MNHKLSETEQYLWNFIEHLILEIPNYSIVKLSEQANVSTATIVR
                     TMKKKGYEGFTSFKHHLKEGENKNINFSFLDKVDKGIRRAILKNEQEVVRTINMLEIG
                     NIEDAIQRIKFADRVFVFARGFSEMIGQEMLVKLQLTGKNCQMHTDPEIIKSISRKLT
                     KKDSVIFVSLNGEKTNKNIKNKIYVFIFQFFK"
BASE COUNT         9385 a         6921 c         5511 g        10361 t
ORIGIN      
        1 ttcttccata cagatgtttt ggcttattct ttctttttct cttcgatcat tgcccctgct
       61 cttatccaac ccgcacttgc cttcttgatc ttgacaggat agcaactaag tttgaagcct
      121 gttgctggca atttgtctaa attcattaat ttttcgatag gacaatatct tttttcaatc
      181 cctgcaaaat gtccttccca gatattcgaa gctccactac gctttcaaaa atatagaaag
      241 agttctttaa tgaaaattat ttcaactttc cataaacagt aatcccatat aaataaaaga
      301 cgtaaaagtc acatgggaga acttatacag gaaaacacat agagtcaggt gaaatacatg
      361 tatcccacct gactctatgc gttttctaac ctttattatt ctctttttat acaatttctg
      421 caccgaatgg catcaacgcc aatcgagcaa gtttgaaaga ttgcttggca aaaggtatcc
      481 ctatgatcgt gatacataga aggatgccgc tgacgacatg tgcgagagca attggtaggc
      541 ctgaaacgat cagccagatg atattaacga tcaatgacat gccagactgg tgataaatta
      601 cttctttgcc aaaaggcgca aaagaaaggg aggcgatctt aaaacattgc ataccgattg
      661 ggatacctat gatcgtgata caccaaagta tccccgctgc taaccagcta agaccaccga
      721 ttagtccgcc gaatataaac cagatgacat ttcctaaaca actcattgta tttctccttc
      781 tattactgtt tttgattaaa actgggcaaa aattgatttt attcttcttt ttttagcttc
      841 cagcatcaag tattctgcaa attttttttg gatttcttcc gtattttctt ttgttactaa
      901 tgtgtgttgg attagttttc ctgatggtcg ctgatccacc cgttcttctg actgttgcac
      961 gtccgcttgc gcaaatccga taatccgttt ttcttttgtt acagcaacga gcaatagttt
     1021 attcttatca tttaggtcag ctgccgtttt tgcttttacg aatgattctg gatgatcagt
     1081 aatcagctga ttgatttgag gtaaatcttc ttttactgcc caccgtattt ccatcatttc
     1141 tcacttttct ttcgctcatt tttatctcca ctaaaaaaat cgactgcggc aattgcatct
     1201 atccagcttt gtgcaatgat ctcatgtccg ccaatcgttg ggtggacccc atcttctctt
     1261 gtatatttcg aaaaaccata agcaatccct aaagcattga atatgccatc taaaggaacc
     1321 agcaatgcgc catattcctt agcaagttta cgaacaattt ggattttagg gtctaagtct
     1381 ttacgccagc catgacggtc gtcggttata ggaagtacaa acggctccat caagatgatg
     1441 ttgcagattt ccgctgtttg aagttcattt agtaattttc gataaacgga ttcaaattgc
     1501 tgcgcagatt tctcagttcc gaatatagcc tgatcgctag tattatgcca ggtatcattg
     1561 attccgatca aaatcgatac atagtcaggt tgtaaagcta aacaatcttc ttgccagcgt
     1621 acttgcagat ctgcaatttt gtctcctcct atcccacggt tcatgcattc aaatttcatc
     1681 gttggatatt gggagattag ttttccggca atcaaaagtg gataaccttt acctagatca
     1741 gttgcttctg ctcggtctct tcctgcgtct gtaatgctgt caccgataaa taatatacgt
     1801 tttttcattt gtcaaaaact cctttttgaa taaaacgatg attttcttcc ttatattatc
     1861 ccataagccc tatcaagacg aaagagaaaa tatatgtata aaaaatgaca ccagcctagt
     1921 cccttcaatc aattgaatga acgctcatca agtgtctagt tttttatact ttttttctta
     1981 acgtgccgga ttccccttga ttccttcgtt gtttggtcca gagaaatcga tcacacctaa
     2041 gcttccgtca atcagaccac tgatgacgcc atcagaacat atatttatag gctcatctaa
     2101 gacttccagt aaatgttctt ttactaatat ctcttctaga ttgcttggga tagattggtc
     2161 ataactttat ctggtccgat gatttcaagg atgaattaaa cttacatatg gatcattgca
     2221 tgctgaaaat acggacctac tccatcttcg aatgttacta ttagtttgtt ttcaggtact
     2281 agatgctgct ttatttcaac agcagaagca tcatcaaatc agatttccat ttgaataacc
     2341 ttctttgtat atgtttgatt gattgcgatt tcgttataga accttgtgaa tcaaatagca
     2401 atttcttttg ctttcttctc ttgtctgctg gaaacttcct ttatcccgta ttttaaaaaa
     2461 agaactgcga gagttcgcag ttcttttttt taaataaacg ttgctctccg gccaactcct
     2521 cggtcacaag tcacaatagt catcaaacat gaaaaactgt tttttgaaat tactccttgt
     2581 gactcggagc tgaacggcag gcgcacaacc tcttgatata aacggtgttc aatcagctga
     2641 ccctcgatat tagctaaaaa aacggtaaaa tatgagaagc tatttttccg ttttgtttct
     2701 ctaatactca ggtcaaggca ctgatttcac aacctcttcc ttaaacggtg ttcaaaagcc
     2761 agtttctcgg tattaagcca aatttatgca aaaatgtgga aagccatttc cacaaaattt
     2821 ttcttaatac tcgaaactaa acagcttttt cacaacctct ccactaaacg gtattcaaaa
     2881 gcttacatct gcggtaataa gtcccgccaa ttcaaaaaca tgaacagctg tttttgcttg
     2941 gctgttctaa ttacttgatg cagaacagct ttttcatcac ctcttatgga taaattcggt
     3001 tcaacaaacg tgggaatgga atagcttcac gtacatggtc gataccagca atccaagtga
     3061 ctgttcgttc aaggcctaga ccaaaaccag agtgcggaac agaaccgtat ttacgtagat
     3121 caagatacca tgaatattct ttttcatcca aaccgtgatt acggatttgt tctagcaaga
     3181 aatcatagtc tgttgcccgt tcgcttccgc caataatttc accgtaacct tctggtgcga
     3241 tcatatctgc acaaattacc acgtcttctc ttgttggatg tggtttcatg tagaatggct
     3301 tgatggcttt tggatagttt aggataaaga caggctgttc gaatgaattc gcaatgaacg
     3361 tttcatgagg tgaaccaaag tcttcgcccc attcgatgtc atcaaatcca tttttcttca
     3421 gtaattcgat tgcatcatcg taagtaatac gagggaatgg caattttgta taagttttta
     3481 agacttcttt gtctcgtcct agtacatcta atgcatattc gcaatgatct aaaacatttt
     3541 gtactaagaa tgctacatat tgttcctgta cttctaaact ttcttcttga tgagtgaaag
     3601 ccatttctgg ttcgatcatc cagaattcga tcaaatgacg tcgtgttttt gatttttcag
     3661 cacggaaagt cggaccgaat gaaaagactt ttccgaaagc catagcagca gcttccatgt
     3721 ataattggcc actttgagat aaataagctt tttgatcaaa gtaatttgtt tcgaatagat
     3781 ccgtagtgcc ttctggtgca gaaccagtca agatcggcgg atcaattttg acaaaatgat
     3841 gttgattaaa gaattcatac gttgcacgaa tgatttcatt tctgatctgc ataatcgcat
     3901 gttgtcttga agaacgcagc cataaatgac gatgatccat caagaagtct gttccgtgtt
     3961 cttttggtgt gatcggataa tcgtggctct cgcttaccac ttccacattt gtaattccga
     4021 tttcataacc aaattttgaa cgactgtctt cacggatttc tccagttact aacacggatg
     4081 tttcttggtg tagattttta gctgtttgga aaacttcttc cgacacttcg ctttttacaa
     4141 ctacaccttg gaaaaaggct gttccgtctc ttaattgtaa aaatgcgatt tttccgcttg
     4201 atcgcttatt tgcgacccaa gcgccgattg ttactgtttc tcccacatgg tttttcgaat
     4261 cgatgatttg aatttgttcc acgtatgact ctccttttat attccttgaa tttcttcagg
     4321 attaattttg acttttactc tcaataaatt gcttgattct gcgtaccgct tcttctaacg
     4381 tagcgcgatc agtggcataa ctcatgcgga cattttctgg tgccccaaag ccttcgccag
     4441 tcacaagcgc tacccctgtt tcttctaaaa gatcttctac ccattgtgtg acattttcat
     4501 acttacacat gcgtagcgtt tcttttacat taggaaaaag atagaaagct ccttgcggct
     4561 tttcaagaga aacacccggc agttctgcca caagcggata taacgtattc aggcgttctt
     4621 caaaggcgtt ccgcatttct tctactgtat cttgctcacc tttcaatgct tcaacagctg
     4681 catattgact gactgctgtc ggattgctag ttgattggga agcaacagcg atcattccat
     4741 tgatgatcac aggatttccc accgcataac caatgcgcca gcctgtcatc gcatatgttt
     4801 tagacacgcc gttgatgatg atggtctgat ttttgattgc ttctgaaatc gttgcaattg
     4861 gagtaaactc attgccatta taaactaaac ggccataaat atcatcagca actatcaaaa
     4921 tatcattttt tactgcccat tcaccgattg cctgcagttc attcttgcta taaatcattc
     4981 cagtcggatt cgatggagaa ttgatgatta cggcttttgt tttttctgaa cgtgcttgtt
     5041 ctagctgttc aacagtaact ttccagctat tggattcttc ccctttcaca aagacaggaa
     5101 gtccttctgc cagttttact tgctcaccgt aactgaccca gtaaggcaca ggaatgatga
     5161 cttcatcttg cgggtcaagg atcgtttgga aaagggtata caaagcaaat ttcgctccgt
     5221 ctgttacgac tgtttgtgaa ggttcataga caagtccata gtttttcttc agataatcaa
     5281 cgattgcctg tcgaagctct gggattcctg ctgttggtgt ataatagcta gctttcccat
     5341 tgcggattgc ttcgattgct gcttcttgga tgttttctgg tgtcgcaaaa tccggttctc
     5401 ccacagtcaa actcagcaca tctctgcctt ttgcttttaa tgccttggcc tttgccgcag
     5461 ctgccaaagt gacagacggt tctaatcgtt ttgttctttt tgataactcc atcccattca
     5521 ttcctttacc ttttaaattc ccttgattgt cttgacttca tctccagatt caaagccaat
     5581 caggtaataa ttcagtttcc catttgtttc ttttcctact acttcccaca caggctgatc
     5641 atcatacatc cctaaagcag ctttttcgat ttggatttca ggatgggccg cagcaattgt
     5701 ctgttttgct tcgttttcag tcagtccctt tgtttgatcc aatactttta ctttttcacc
     5761 agatttagga atgataacgg cgatttcttt tcctttatca tttgtacctg tcagtgaaaa
     5821 gtatgtcttc tttcgagtaa accaataaaa atgatcggtt tcctcaagat ttgcatattt
     5881 ctttgcgatt tctactgctt ctttcttcgc ctgcaccgcc ggacgactgg aacgcaagta
     5941 aaaaatcgag ctgatcacga tgaccgctaa taaaaatacg atcaagccaa gtaacacgcg
     6001 attaatcgtt ttttcctttg tctgtttttt cttcaagggt cgtcgctcct ttatcttcat
     6061 tccttttcat tatagcagat tttaattatg aaaaaagaca gctcctctaa cttccttctt
     6121 catttttctt taagaattca tggatatcct gatagatttc ctccatagct agctctttga
     6181 tcggtacttt tttcggtaat gcatggatga tccgtgcgcc atattttgca gtgactagtc
     6241 ttcgatccaa aataagcaat acgccacgat ctgtctccgt ccttatcaag cgacctaatg
     6301 cttggcgtaa cttcaaagct gctttaggta cggattcctg gtaaaaagga ttgatcccct
     6361 ctgattctaa ataagcgttc ctagctttga ccatcgggcg aagcggatta tcgaagggta
     6421 aacgtgtgac tacaacgatc tgcaaagcat ctccaggcaa atctacacct tcccaaaagc
     6481 tgtcagcgcc aaacaatacg cttttttctg acaaaatgaa gcgtttcaaa agcttctccc
     6541 ggcttccacc aaacccttga gctagtattt ctcgtccttt ttctagcata gacagatgca
     6601 ttttctgata gaccttctgc aaaacatcat gagaagtgaa taacacaagt attggccgat
     6661 cttcttgttc gatcagacct gtaagtgctg tctctaaata atcgatatat gcttgattgc
     6721 tgacttgatg gacggcaggt gcctcttttg gcacatagat ccttgcctgt ttctcataat
     6781 cataaggaga ggcaatcact ttaagaggtg ctgcttcaat gccaagcctc ttagagagat
     6841 agtgtcgatt agggccgatc ttcagcgttc ctccaatata aagaatacga tcatatcgag
     6901 gataccaaac agtcgacggc aacatagccg cttgaaaatc atggagatga aaaatcatct
     6961 gctgcgctgt gttcccagga acgaaccaat ggatcaaatg atctcgccag tcttccagcc
     7021 attgatccat cagcaagtat tgcttgtcta attgatcaaa taggctatat aaacttgcta
     7081 aatcgatccg catctggttt gtccagcttt cttggataat ctcaaaagtc agacgcattt
     7141 gtgactgtag ctgctggatc tcctgataaa aaagctgcag gtgatcaatg gctgtcccag
     7201 attccagtgg cagctgatcc aacatctctt ttgtcatcat gacttcttct ggatactgtc
     7261 cgttaggttg cttgccgctc actaaactca ttaatccttc tgccatacaa atctgtgctt
     7321 cgcatagcgc agaaagttct tctcgataaa tcgataacca gcgtaaggct tcatgttctt
     7381 tcggtaaata gctttgtatt cggtcaaaca gctgatttgg ttcttctaat tgctggattt
     7441 gtttatggaa atagttaata cttaatcgtt tgccatttac tttttcagcg atttccggca
     7501 aatgatgtac ttcatcgatc aacaaaaatg gactagctgg aagcaatggt tcttttcgct
     7561 gtgtttcttg tactaaaaac gcatgattga caatcaaaaa attactttgc ctgattttct
     7621 tttgcaagaa acgaacaaag tcttgttcgt aaaatgactg atttcttgct aaaaatccaa
     7681 tacctctgtg cgcgatatct gcaaagaatg aatggttcaa atttgttaaa tgtaattcat
     7741 ccaaatcacc agtcttcgtt tgcgtcagcc aaactaagac cgccatttga tacaaagcat
     7801 attgtttttg ttctggcggt tccacaagtg tgcctttaaa acgctgcaaa tcaatatagt
     7861 ggcgatagct tttgataatt acaggttgta tgggctgatc tagcaattga ttcagcaatg
     7921 gaatgtcttt ttctatgatc tgctgctgca aaactagcga aacggtactg atgacagcgg
     7981 gtttttcagg tgtagccaaa taactcaatg gcagcagata accgattgtt ttacccattc
     8041 ctgtagctgc ttcgatcatc aaatcttttg attcatcttt tgtgaaatgg tcgtagaccg
     8101 cattcatcag tctgttctgt tcttttcgat acatcagtgt tttaccaaac attttttctt
     8161 ttgcttgttt ttttcttgga tatgtccgct cgccataatg gacggatgtg aatagttcca
     8221 cttctttttt ctgcaaagct agcccatcga cgatttctaa tgagggatca agtggctctg
     8281 gtttttcttt catttcttca atcacatgat aaatgaaacg actcgtatcc atacctgtgt
     8341 gtctacttaa tcgcgcaatc tgctctaacg tgatgattgg caattttctc atccgttctt
     8401 cgatcagcaa aagaagttga cctgtcactt cggcatcact atctgcctga tgaggattct
     8461 catgaataaa tccaaggctt tctgacaaat cagctaagcg aaagcttggt tccgtcggca
     8521 agaaaatttg tgcaagttct actgtatcga tgcctggtat cttcaaacta ggaagtccgc
     8581 atcgcatgag ttcatggttc aaaaaattat aatcaaaata aatattatgc gcgacaaata
     8641 tggtgtctgc caaaagattg taaatcgttt gggcgacatc ttcgaaataa ggcgcttttt
     8701 ggacgcgttg attcgtgatc cctgttaaat gctggatttg ttttgaaatc ttccgccctg
     8761 gatgaatatc gatcgaaaaa cgtgaagtga tccgacctga ttcaatcatg acgcagccaa
     8821 attggatgat ccgatcttct tttggattcg tcccagtcgt ttcgatatca acgactgcat
     8881 aagttcttgg tttcaaaata tcttcctctt ctcctgcctc taatgttctt ttgaaattat
     8941 actacaaagt gcagaaaaat actctcatcg ctttttaaag aaaatagaca aaaacgccca
     9001 gacacgcaaa tgtctgggcg tttatttttt agtataggtt aattaagctt tagtaacgtt
     9061 tgttgcttga ggtccgcgtt ggccttcttc aacatcgaaa gttactgctt ggccttcttc
     9121 taaagttttg aatccgtcgc cttggatagc tgagaaatga acgaatacat cgtttccgtt
     9181 ttctgcagtg atgaatccaa atcctttttc tgagttaaac cattttactg tacctgtttc
     9241 cataataaaa atcctccttg tgtatcaaca catattttgc aattacttga atggtgtata
     9301 tctggaagat gtttatttga aacaagctac cacagattac cgaacaaaaa ctactcctat
     9361 agtatatcat accgtttaca aaaaagctag agtttttcga ctttttttat taaaaaaagt
     9421 tttctcagcg attacataaa gtgaaaaacc gaactttttt ttaaaataaa cgacatattg
     9481 ttcagttggt gtttttttgc tctgatgttc ttttttaaac taaaaaaatt attctttctg
     9541 actacttatc ggttctgttt caacggtcga ggctggggaa gattctttag taggtctgat
     9601 gttctcatgg tatttctgat agcctatgac agttaccaga agaacaaggc aaaccaatac
     9661 gaccaacgag attagttttt cttttttgct catatctata cctcataaat aacaggatga
     9721 tgtccatcat atttctgagt atgagtaaaa gcaaaagctg cagctgtaga atgtaagaaa
     9781 tgccagcaac tttcataatc acaggtcgga cagtaccaaa agtaaccaaa tcttggctgc
     9841 aaagaccgtt cttctgtttc aggaatcgca gaatcaagat ctggtgtctg tacataaaca
     9901 tttttgtaca gacgtgtgcc tgtaatagga aaccgctgtg tttcttcagc aaaaactggc
     9961 tgacttgtta aacttaggga tagagacagc aaacaccata acagtctttt tttcataaag
    10021 acctccttat cgataagttt ttcccaatta aaatcatatc tcaagaacgc ccgttattca
    10081 cattttgttc ataaatgatg ttttttgtat cataatcgtc aaaaagaggt cgtgaccacg
    10141 tcacaacctc ttttttaaac ggtgttccag aagtagcttc ttcggaaata agccgaaatt
    10201 tcacaaaaat ttgagaaata atttttgtca atctcttctt atttcttgaa gctgaccact
    10261 tctgtcacaa cctcttcctt aaacggtgtt caaaagcttg catctgcggt aataagccca
    10321 ccaaacaaaa aatatgaaga gctatttttg cttgctgttc ttattacttg atgcagaaca
    10381 gctttttcac aacctctcat acacggtgtt taatcagctg accttcggta ttagctcaaa
    10441 aaacggcaaa atatgtgaaa ctatttttcc gtttgtttct ctaatactca ggtcaaaaca
    10501 ctgatttcac aacctctttt aataacgaag aatcgctgtc agactctttt catctggcgc
    10561 atctttttga tccaaaataa acacttttcc gccattaatg atcacatcat ctgctaatgc
    10621 attcaacact tgacgccgat catattctgt ttcaggatct tcaccgaaac catcagctaa
    10681 gtttgacgta gcgataaata aatgtgacac ttttccttct ttcgcagctg gaacgatatc
    10741 aactaactga tcgatgaatt tcttatctaa gagtttattg tattcttttg attcttgagc
    10801 tgcgagttca cttgacagct tcttcgcccc ttcttcgata tctctaaagg atagctgcgc
    10861 aggagaagca gaaatcgcaa cgtctgcaca ataataggga tttttagcaa tttttttgaa
    10921 catcgtctga ttttctggta gagcaaataa ataaatcggc cattgttctg gattttcgaa
    10981 agaatccttc aagtacgtat caactgcttg gtaatagttg acccaatcga tctctacttc
    11041 ttcatctttt ggatttacgc catggaaagt tactccatct cgggctccgc ctcctccatt
    11101 agaagagaag ttcaaaccgc cacctgtcag ttcagatccc aatgcagtaa cgacatcaac
    11161 cggtgcccct tctggtagag atacttcttt tacttgtttg ttgctgactt catacaattt
    11221 catggagtcg cggttcaatc ccaataaata ataagaatag ttgaactgcc catttttgat
    11281 aatagccagt aaataaggaa gatcacttac atagtattga tcgtctaccg ggatactcaa
    11341 acgatggata taggtttggt cagcagttat aatgaccgct acactcgtag ttgcactacg
    11401 ccaaaacgaa ggatctccta acaaattttc aattttttct tggaacgggg accagtcatg
    11461 ttcggaatat tttttctcaa aacgtttctt tgcttctttc gcaaaatttt tgaatttgat
    11521 ctgatctttt tccacatctt gatgggcgac atgcgtattc agcatgatcg tgacgaatgg
    11581 tcctttagcg ccttctgaag tcaattgtga caataattct ttatcctttt ttgccataaa
    11641 cgtacccctc ctaaaaaaat cattcctttg tttctatcta aagtctacca caacactatt
    11701 tttccataca ataaaacgta tctaaaaaaa taaaacggtt tctacggttt taaatttagc
    11761 tattttttta agttatcttc ataatttcct caattattta gccccagtct tcaactattc
    11821 ttcatgaaat gctgataaac taagaaaaag aataaaagag gtggatctta tgcatttgat
    11881 tatgattttc agtttattaa gtattatcag tttatttgct ttcagcgctt cccgcttatt
    11941 agatgcggaa acacagccta ttcctgtaaa agtacgtaaa aacgaccgct aaccttccat
    12001 aacgtgcgtt cttatcctaa agttttttca gaaacattga aactctctcc cctctctgat
    12061 aaaattttaa tttgtagcga aacaaattaa ttacaaggag atcggatatg tccataaaaa
    12121 aaacacgtct agtcctatta ttggctagtc tcattctttg ccttgcttct tttctaccag
    12181 ctgttgctgt gcaagctgaa gatactttca aagtaaatgc aaaagcagcc tttgctgtag
    12241 atgcgcaaag cggtaagatt ctttatgatc aagatggaga aaaaaccatg gggatcgctt
    12301 caatcacaaa aatcatcggt ctttacatcg tccttgatca agtaaaagaa gggaaactat
    12361 catgggatga taaagtttca atttcagatt atgcagaaaa tttgagtgtc accccagatt
    12421 tatcaaatgt tcctttgcat aaagaaaaca cctacaccgt taaagaatta ttcgactctg
    12481 caatcatcca gtcagccaac gcttcaatgg ttgccttggc agaaaaaatc tctggcagcg
    12541 aagcaaaatt cactgaacga atgaaagaac aattgaaaga ttggggtatc aaagatgcaa
    12601 cgatcgttaa tgcatcagga ctgaataact cttatcttgg cgaaaaccga ccagaaggta
    12661 ctggagaaaa cgacgaaaat caaatgtctg ctcaagatgt ggctatcgtt gcccgtcatt
    12721 tgatcttgga ttttccagaa atactggatg tatctagcac tacgacacaa atgtttggcg
    12781 aaaacacgca atcaccagta gaaatggtca actggaactg gatgctccca ggattcgtga
    12841 attataaaga aggcgtggac ggattaaaaa ctggtacgac tgaacttgca ggagcctgtt
    12901 tcgtcggtac gatcactaaa aacgatcaac gaatcatcac tgtcgtattg aatgcagacg
    12961 gccatgcgga aaacccaagt gcccgtttca atgaaacagc tcgcttaatg gattattgct
    13021 acgacaattg gtcagaaaaa gagcttggaa aagccaatgc aagtatccct agtctaaaaa
    13081 ccattgatgt aaaagacgga aaagaatcaa cagtgcccgt tgtgcttaaa agtcctgtaa
    13141 aagtatgggt acggaacgat atggatactg gaaaattaac aatcacacca actcttgaca
    13201 aaaaacaagt cactgacaat gagatccaag cacctatgga taaaggaaca aaaatcggta
    13261 ccgcaacgat caccttggca gaagataaat taggttatct tgaagacaac aacgcgccat
    13321 ccacagaaat catcacggat aaaaccgtag aaaaagcaaa tattttcgtt attggctggc
    13381 gacgcgtcgc agaatttttc ggtaacttgt tttaattgaa caaaaagaaa aaagcatctt
    13441 tccatgtgga agatgttttt ttgctttttc tagtttaaat aacagctttt ttcatctatt
    13501 ttcgttatac tgtataagaa aaaggagttg aatttatgaa tctaaccaga gatctgattt
    13561 ttttggttct ctatcttatt ggggtattct ccatcgttta ttggcgaaaa cagaaaaagg
    13621 atccttcctt gttcttttac agcagtacat ggacgtttgc ggtaatcttg acttattttg
    13681 tcgtgttgga aattttttat tcgacctttt attttttgat tcccttagtg tttttcagta
    13741 tctttgcctt ttcctatttt tcagaaaagc gaaggctgtt gaatggtctt ttattcaata
    13801 ttttcctcat tagttttggt atctacttgt ttgtcttgct atacgaaaca caaaatattt
    13861 ttcttggcgg actgattgcc ttgatcacga tcccgttact gcttgtcttt ctattcggca
    13921 tttatggatt gatcgttttt cttttttgga acggtgtcac agttttacgt agagagtcgc
    13981 attcattagc taatctttta acgttgatac tagccatttt tcttacattg tttcttgttt
    14041 ttgacttttt cttgttaaaa tatttaccac aatggataaa tgctttgttt ttctgcatcc
    14101 cattgatctt gatctatcta tttatcgtat tctacaactt tttgactgtc tcattcttgt
    14161 atcagttcaa ccgccctcga tacaaccaag acttcatcgt tgtgttaggc gccgggttga
    14221 tcaatggaga aacagtttct cctcttcttg ccaagcgaat caataaggcg attgcttttt
    14281 attgggcaca aagccgtgcg acattaaatc cgcctatcct attaatgtcg ggtggacagg
    14341 gagcagacga aaaagtacca gaagcaatgg cgatgaaaca atatgccatg gaacaaggaa
    14401 ttcctgaaag agatattctt gtcgaaacca attcgacaac cactttggag aatatgcgct
    14461 attcaaagga aatcatggat cagcaaatga aaggtcccta tcgcgcgatc ttcagttcca
    14521 acaattacca cattttcaga gctggtctct atgcacgaca agcgaaactg aaggcaaatg
    14581 gcattggggc aaaaacagct ttctattact tgcctaacgc gtttttaaga gaatacatcg
    14641 ccatcttgat gatgaacaaa aaaagacata tgatcatttg cggattgctt ttcgcaggtt
    14701 ccgcattctt atcactgatc actttacttt tctaaaaaaa caaggtgtga caaaacatat
    14761 gtcacacctt gtttttttct tttattctct tgacgattgg caagaatagt attccttttt
    14821 tgacggctac ttgctgtgca gaataaatac cgctgtttcc tgaacacata aagctgatca
    14881 agcagatcat aaagaagtaa gcagcagctt gtgaaccaaa taattcgatt cccatcacaa
    14941 aacaggcaat cggtgtattt gtcgcaccag aaaatacccc gataaaacct aatccagcaa
    15001 gaaaaggaac agaaacatgg agcaaaggcg ctagtactgc gccaagcgtt gcaccaatct
    15061 caaatagtgg tgtcacttct cccccttggt accccgcacc taaagacaag accgtgaaga
    15121 ataatttccc aataaaatca taagcatgtg cctgtccatt aaatgattcc tgcagtaacg
    15181 gaagactcaa cccaaggtaa cgttgtgttt gcaaaatcaa cgctgcggct acaactactg
    15241 ctccacctat cgtatttcgc caaacaggat gaggaatcca ttgcgcatac cgcgctttta
    15301 aaaagacgat cgaacggctg aatacccaac caatcaaacc aaaagcaatc ccagcaacaa
    15361 aaactttgaa gaacagcaac atactccatt caggaatcac acccatttgg taatgtagat
    15421 gagaaacacc atagctttct gtcatgaaat ttgcaaaaag cgcagcaaag aagctaggaa
    15481 atattgcttc tgttcggact ttaccgattg ccaacacttc taatccaaat aatgttccag
    15541 ctaaaggtgt gccaaataca gaactaaaac ccgcactgat tccactgata accaaaatct
    15601 cccgttctgc tttatccaat cggaataaat gcgctatatt atcagctaat gctcccccca
    15661 tttgaactgc tgttccttca cgaccaacag atcccccaaa taaatgggta gtgatggtcc
    15721 caaacaaggt caatggaatg agccgcaaag ggatcttctc ttctcctcca ttcccttgat
    15781 cgatcactaa gttattccca cgactagaaa gaccgccatg atacgcataa agataagcaa
    15841 aaagcgcgcc gctgactggc aatagataaa gtagccaagg atgtgctagt cggatatctg
    15901 tcacgaacgt caggctattt aagaaaaaag cagacaagct ccccatgaat actccgagaa
    15961 tcgctgtgac caccagccac ttcaacatgt acatcaaaat acgcactgga ctcggtaatt
    16021 gtttttccat aaaaaattcc tcctcaaata aaaaaacgcc tactaaagaa aggggctggc
    16081 cccagacttt agtaggcgtc attagctatc ttacgttagc atttaaaggc gaacaccatc
    16141 acctattcac ttccattaaa agatagcata tttttctgta aaaaacaata aaattatcgt
    16201 tatttcttta cacgttcagc aaattcatag aaaggagcca atttcgttaa ctgttgatta
    16261 cattgatcac tatcatgaca aatataattc ccctttttcg tatacgtgcc atcagaaccg
    16321 gctttcgttg tagaaagaaa gagcgagaca ttcgatgttt tctgacagat cgcacagatt
    16381 cccttattaa tcgttggtga taaagtaccg ctgatccctt ttaattttcc atcttcgtaa
    16441 taaaccatga attttttttg cgtccctgga tcattccaac cgatgtacgt atgtgcttgc
    16501 aagtcgattt cattccaagc gggttgtttg agttttttca cttttcggaa taatttctcg
    16561 atttgtggtt tgctcggctg cataaacggc tggacgattt gcttcagctc ctccaatgct
    16621 cgttctgctt gcgtttttgt cagttttaca tctaaaactg tcgttaaaaa cgtatcgatt
    16681 tctgatggtt cgtaacctaa tacatcgcga atcttttcag ctgctagagc ctgaaccgtt
    16741 tgtaccgttt ttgagtcatt gacagatgag tacgcattta ataactgctc tacttctctt
    16801 tttataaaaa aatattgata tggtttcatt gtttgttcca tgattttaaa gtcctttctt
    16861 ctaaccaaag tgcatacttt gtagaaaaaa gtgatcttct gcaaagtatg tgttacctga
    16921 agatcccgga ctcttttaaa taagatacgt tttgcatctt atcatcaccc cctttaatgt
    16981 tcattcttct atgtacatga ttcattcgat aaataaaagt aaaccgtatc ttgagcagat
    17041 agtcaactgc aaacagaaaa aggactgtga caaaagtcgt gtcaaagtcc ctttctacaa
    17101 acaaacggtg ttccagaagt agcttcttca aaaaataagc cggaatttca caaaaatttg
    17161 agaagcaatt ttcgtaaatt ccttcttatt tcttgaagct gacccacttc tgtcacaacc
    17221 tcaatatggt ctattattgg ttttacctgt taaatcgagg attcttttcc gaagtaacgt
    17281 tgccatctct tacgcatact tgctgacata ggctgagcgg cttctttgac tgcgcagtat
    17341 ttgtctacga atgttacgat atatccttct ttgtacttag gcggtgcaat cgttgcgccc
    17401 cacatgtgct tcaaaatgat atctcgctca agatccgaca aatcggtgat cttttctgca
    17461 ttctttacag cgattcttgg atggatatac gcatgtgtgc cttcatcaaa tttcgtcgta
    17521 cgccaatcat agtagaacag atcatgcaaa agtccagcac gcgctgttgc tttagcattg
    17581 ccaccccatt tttttgcgag cagataacta tagtaagaaa cactgatgga atgctccaaa
    17641 cgagtggaat gaacatgctg tgtgtaattc gctaattttt tcactgcttc tgtttctaaa
    17701 agatcttcta cataagacat atattcttcg tcttcacgcc atttttctgt cataattatc
    17761 gatctgccac ctttttgaat ttcattttct tctgaaattg atctaagtat accaccagtg
    17821 ttcggattat tctagtcgcc ttggataaaa tagccaaagc ttaatagttc cttaatcata
    17881 aagaaaaatg ataaagaaca atccctgccg caaccccaac attcaacgat tccgcatttc
    17941 ctttcatcgg gatatatacg ttctgatcag tcaaagacaa gatttcctga cttaccccct
    18001 gaccttcgtt ccccatgatt attgcgaagt tatctgattt ttctactttg tctaaggcgt
    18061 gtgcttcttc atttaattct gtgccataca ctgtaaaccc tttgtctttc attaaaggaa
    18121 tcacttctgt caaatctttt tgcaacacag gcaaatgata attgcttccc tgcatgctgc
    18181 gcagcacctt tgtactataa atatctgccg ttcctttgcc taataaaaca ccagccatat
    18241 caaatgcatc tgccgtacgg atcatcgtcc ctacatttcc aggatcttgt acattatcta
    18301 ataacagcca tcctcctgaa aaatctggcg tgccttcagc cgttaacgcc atcactgcta
    18361 agattccttg tggtgtaggt agatccgata cagcatccat gacctcatcc gaaactagaa
    18421 aagtagaaat ctctttgttt gcggccagcc agtctcccca ttctttcatg cctcgctgtg
    18481 tcacaaaaag ttcttttatt tctgcatcag acgacgcagc ttcttcaatc agatggaacc
    18541 cttccaaaag atattcttcc ttttcttccc gatatttctt tttatgtagt ttttttaatt
    18601 ctttgattct tggatttttt gctgattgga tctctttcac attcctcacc tccccatgat
    18661 tatatcatgg aataggtgct tgagcttgtt tcatcctaaa aaaatcggta tcattaattt
    18721 ataaaatagg aaagaaaggt gacgaacatg cgcaaagtta aaatgaacgt ccaaggacgc
    18781 gtccaaggcg taggatttcg ctacatgacc aagatggtcg ctgatcaact aggcgtgact
    18841 ggaacggtaa aaaacgaaga tgacggctca gtcacgatcg aagcaatcgg caatgacgac
    18901 atcatccaga aatttattga agaagtcaag aaatctccga gcccaagtgg acgagtgcag
    18961 tatgtggatg tccaagaaga cccgatgatt gaggaacgaa agaaatttga tgtggtggga
    19021 taaggttgtg aaaaagctgg tctgcatcaa gcattaagga caggttgtga aaaatagctc
    19081 tccatatttt tcaacaggct ggacttaatg ccgtagatgc aagcttttga acaccgtcaa
    19141 taaggttgtg agaaagtcgc ttagttttga gtagtaagga gaattttgtg aaaatggctt
    19201 tccacatttt tgcataaaat ttgacttaat accgagaaac tgacttttga acaccgtcga
    19261 taaggttgtt tttgagacgc tttgcctcaa gtattaaaga taggtagtaa aaaagctgat
    19321 ctataacttg caataaattt gcttaaccca tattttccag acagaacaaa aaagttttta
    19381 gttttttaaa taagaaagaa acacttggaa tcactctttt ctttccaagt atttctttct
    19441 tgattttttg tctttttttc aaaatttttc gctacgtaaa agaacgaata tttatatgaa
    19501 atttctatga agttgattga attccatgcg cttttttagt atagttaagt ttgtgtaaaa
    19561 attatagaag aggaagtatt ctatgaataa atggaaaaac tggttattgg gatccggact
    19621 tgtcagtatc ctgctctttt tatctggctg tgtgcggatg gattcaaacg gaaaccctga
    19681 cacttcggga atcgtttatc gagtactcgt ccatcctatg ggccaagcga ttacatatct
    19741 agttgagaat ttcaattggt cttatggctg ggcagttatt gcaatgactg tgatcgtccg
    19801 tatcatcatc ttgccactag gtattagtca atcgaaaaaa acaatgatcc aatctgaaaa
    19861 aatgcaagcg ttaaaaccac aagttgaagc agcccaacaa aaattgaaag ctgcaacaac
    19921 acgtgaagaa caaatggcag cacaagcaga aatgcagcaa gtataccgcg aaaacggatt
    19981 gagtatgacc ggcggaatcg gctgtctgcc tctattgatc caaatgccga tcttctctgc
    20041 cttgtacttt acggcacgtt atacagaagg tattcgggaa tcatctttct acggcatcga
    20101 tttaggctca ccaagtatgg tacttgttgt cattgcaggg gtcgcctatc tgcttcaagg
    20161 atacatctca acgatcggta ttcctgaaga acagaaaaag acgatgcgta cgatgctaat
    20221 cgtctctcct gctatgatcg tcttcatgtc gatcagtgcg ccagccggtg ttacacttta
    20281 ctgggtagtc ggtggtgtgt tcagctgtct gcaaacattc atcacaaacg ttatgatgaa
    20341 accacgtttg aaagcacaag tggcagaaga gttgaaacaa aacccgccaa aacaagtcgt
    20401 cactccaaga aaagacgtga cagaatcagc aaagccagct aacaacaaac aagcaacaaa
    20461 acaaatcaaa caaccaaaaa acagtacaaa caacaaaggc cgcaacgcag gaaagcagca
    20521 gaagcggaaa taaaaacata ggacaaaaaa gatgagagct aagccctcat cttttttgtt
    20581 actcttcctc ttctgctatt ccattaatta attcgttgac agagacaatg ctttcacatc
    20641 ctgcacgaac aaactcgggc tctgaaccaa tcatttgtga gttgataaat tcaatgacca
    20701 tcggaagatt catcccagca taaagagtaa tctgttgtcc ttccattatt agtttagaaa
    20761 tgatatttgc aggtgtacca cctaaaagat cgcatagtac cacaaaatca tttaacgaat
    20821 ctgtgacagt gagaaacttt tgtttgtatg tttcagtgcc atcttctggt aataaaggta
    20881 acgtgtagat atcctcttga ggccccatga tcatttctgt actttttttc aattcctcac
    20941 aaaattttcc gtgactaatc aataccaatt gtctactcat ttttcgctct cccttatttc
    21001 gaaatcacac ccaatgcaga taaagcaaca cacacaatta atacgataaa aatcgctcga
    21061 gtaggagtca tgtttttctt tcctaataac caataaacac caccgacgat cccagcaggt
    21121 aaaattttag gtaagatcat atcgatatag ttttgcatat tcaatgtcac atcgccaatc
    21181 gttggtgccc atgaaaattt aacattgatc attgtcgcaa ctaatgcccc taccatgaat
    21241 acccctaata aagtggctgc gtccgttaat gcagttaagc gagattgcat agtagtaact
    21301 aaggaaattc cttctttgta tgcaaattca agttgcttcc aacgaaaaac catgacaacg
    21361 atctgagcaa aaatccatat aaacacacct atcggatttc catcagttgc catattcgcc
    21421 gccaaagccc caaaaatcgt tggaatcaaa gcagcaaata cggaatctcc aatcgctgca
    21481 aaggaaccca tcaatccagc tttcagcccc gatacggttt cctttgaagc aattccctct
    21541 ttttcttcca ttgcaagatc aatcccagta atgattgtat taaaaaaatt agaagtatta
    21601 aaaaattgcg tatgtgtttt catcatctct tttaattctg gtgtattgtc tccatatatt
    21661 tttcttagtt gtggcaagat agtatataaa tagccagaac cctgcattct ctcatagttc
    21721 caacctaatt gaaaagtgaa caaacttctt cgattgattt gcttaaaatc ttcttttgtt
    21781 aatttgtaat tagatttcgt catcttcgat ttccccctca acagaatgtg atggctcact
    21841 agctaatggt tgatggtctt tgttttgatc attcttataa ctgatcattg ctagtgctag
    21901 accaatcaat gctacagcaa gcatcggcag cgaattgaaa gttgccggaa gatctttaac
    21961 aacgcttgcc acacttgttc ctaataattg aacattagag aataacgttg ctaataatgc
    22021 cgtgatcgta aatcctaaaa taagataaga tagatgtttt tttactggta aatagcgcag
    22081 aagaatggca aacccaacag ctggtaaaac agctcctgct actgaaagtc catcgcctag
    22141 ccattttaga ttcccgttta aaacagaaac aacattttca actaatccac caccaaatgc
    22201 taaagctaaa aatacaggaa tcatacgaga gagcgaccaa ggtaaagctc ccattaagaa
    22261 attgcgttcg attgcttgat aattcatgtc ttctacgtga cggtcaatac gatgggcaaa
    22321 gtatgtgtta gcgaatctag ctaaaatatc caattggatc attaagcttg ctacaggcac
    22381 tgcaatagca gcaatcgctt gttctggatt cattccaaga gaaatcgaga aggcagttgc
    22441 taaaatcgtc ccagagttgg catctatttt tgatgcgcct ccaaacgtcc ccactcctaa
    22501 aactgtcaac tgcatactgc caccaataat caaacctgcg tttaaatcac ccattactaa
    22561 acctgcatat aaccctgcaa acactggcgc actcattgaa gaatagatct gtaattcatc
    22621 taaaatttga taacctgcat ataatgttaa taataagatc tgccaccatt gaatatccat
    22681 ggatcattcc tcctatttga ttaaaagatt cataaagtct tctgctttat cgtttggtac
    22741 catttgagca attaaatgta taccaagctg atctaatttt ttaaatactt cgatatctgc
    22801 atctaccaca ttgattgatt ttgtaatgga ccgtgtttca ttcgtttgtg acatattccc
    22861 cacatttact tctttgatag gaactccttt ttctactaaa gataagattc gatctggctt
    22921 ttttgctacg atcaatactc gttgagagtc atatttgcca gctaaaatat ttgttgctgc
    22981 tttttcgatt ggcaaaatgc ttaattttac ccctgctgga gtcgctaact tcaatccact
    23041 tttttcaatt gtgtttgtgg ctacctcatc atccacgacc atgattcgac taatattcag
    23101 tttcgttgtc caaagatttg ctacttatcc atgtatcaaa cgtccatcga ttcttacacc
    23161 aataattgcc atcatgcatt ctccttatct attttttcta tttattgttc aattaaaact
    23221 gtgacatcat caaatatccc ttctgtttca aagataacgt tttcattttt tctttcttat
    23281 atttcatttt taagctaaga acaaattcct tcccaactaa agaaaacgct ttcttttata
    23341 tttaagttca tttgattttc ttaacttgaa tgtgtttcat caacatcgta actttcatag
    23401 ggtcgatcca gccagttttc tcttgttgcg cgtttgccat accacaagcc atagaaaatg
    23461 cgagaatctc ttcgattgat tgcctttttg ataacgcatg ggccgatcct gcaagggtcg
    23521 cgtcacctga accaacagga ttaacaactg atattttagg aataatgact tcgtaaaaac
    23581 actgagcaac ttttgccaca gcacctctct ctcctaaaga aacaacaacc catggcactc
    23641 cttcaaatat tggatgagct aataaggact gaattttttc taattgaatc gaagaacaga
    23701 tacgttggcc aatcagttct cctaattctt ccagattggg cttgataagt agtggcttct
    23761 ccttaccttc caatacttca agcaaaaatt taccagaagt atctagtaat atgtctttcc
    23821 cttttttatc agctaaagca accaattgct tatagtaatc aagagggcat ccttggggta
    23881 aactacctga gatcgtcata atatctgata tttcgaggag ctgctcgaaa tgatcggtaa
    23941 atgtctgttg ttctttcttt gttatgaagg ggcctcgttc caaaagctct gtttgtctcg
    24001 aactatgcag gatagcaata gaatcccttg tctctccttt aattggaaca aactgatgca
    24061 tgatccctaa ctgatctagc tggttcttta gatattcacc gtgctggcca cctattaaac
    24121 cagtagctat cacctcttct cctaataaat ttagaaccct cgccacattc actccttttc
    24181 ctcccgctgt tttattgact gtttcaactc gattgacttt gtcgaggttt aattcttcaa
    24241 ttgtatacgc gatatcgatg gaaggattca aggtaactgt cgtaatcatt tcatcttcac
    24301 ctttttgctt tttgttttat tgaaccaagg ggtagcggtt tcatttatta ctttatttaa
    24361 agtctcgatg ttttctctcc catttgttct taaccaatga atagctccgt cttttccatt
    24421 ttcgacgtat gctttgaccc catctttcca tgtcgcacgt ccacacaata caccattgaa
    24481 ggtggaaccc gattcatgag caaaaatcag tgtttgttga aataattctg ttgaaacacc
    24541 cccactcaaa aaaatgaatg gtaatggagt cgcttcactt tgctccttaa agaaggcagc
    24601 agcctcttct ttgctgtgaa taaattcacc tgtggaatag ccattcacat aattcatatt
    24661 gactggtact tctactttca acacatcaac tttatatttt ggatcagaaa attctttcat
    24721 catctcaata actttgtggg gcttgacttt cgcatactct ggagaagaac tatcgcttat
    24781 ttgtgcatca taagaaacta actccaaata gaagggacga ttttctcctt cgcactcact
    24841 acctaaacgt tcgatgaaca catgcttcaa atgattgatt tcgggtgctt catcaacatc
    24901 gtagtaaagt aagaatttta ctgcatccgc ttgctgttct ttcaatctga gagaagacca
    24961 atccgctaat aaatcaggta gacgaccagg cgtagttgcg tcatagccgg ttttctcata
    25021 agctaacaat aaccccgtct cttttgctct gacttttgct gctggcaatc catattccgg
    25081 atccaataaa atagcagaag catagggtgt cagctcagct gatactagtt ctttgaattc
    25141 actgatttgg tcttcacttg gctcttttcc caacgatttg atcatttttt ttagagcacc
    25201 acgctgatcg atcgctaatg cactaataat cccatctttt gttgatagtc tctccattgc
    25261 cctatttttt tcttcattca tctgtcattt ctccttcggt ttcatactta tgaataatga
    25321 cgcccttgac tactcgattg accgtgcctg ttggagatgg tgtatctggt ttattagcta
    25381 ctttgattga agtatagagt gcaatgattt gtgcagtcat aatcatcgct aatgcaagat
    25441 aggcatcggg caatttactc accgagtctt ccaatagcac agtcatacct gagaaatcaa
    25501 tagtacccgg ttgcgcaatc gcaatgacat tggcagtgac ccgatcttct tttatttcct
    25561 ctaaaatatc taagtcataa tttcgcgtat atggttgatt gctcacaaaa cctacaacga
    25621 gtgtctcgct atttaaaaaa gactttggtc catgacgaaa ccccatcgaa gaatcaaaaa
    25681 ttgtagcaac tttacctgct gttaattcta aaatttttaa ttgtgcttct cttgtcgcac
    25741 ctgccaaact tcctgaaccc agataagcga tccgttcaaa aaaatcaggt aataactttt
    25801 gaatttcttg atctcttgaa agaagatctt gaccgatttt aactaattgt tcaagatatc
    25861 tttctttttt ctccattgct gtttgatcaa agatccataa agcactcaat aacatacacg
    25921 taaaacttcc tgtcatagca aaacctgcat cgtttgctct ctctggcatc aataacagat
    25981 aattctgttt atcatcctgt gcgttttttg ccaacatccc ctctttagca caagtaatcg
    26041 tcaaatgaaa caatgtttca acttgttcat ttgcaatttg cacagccgct acactttctg
    26101 gactgttgcc actgcgagca aatgaaacta ataaagtcgg ttcatttgga aacaaaaatt
    26161 cttttggtga agctacaata tctgtcgtgc caaaactgtg gaagataaaa aactgattgt
    26221 ctccatgttc atttagatag ggtaagagcg tatccccaat atattgagac gttcctgcac
    26281 ccgtaaaaat aacacgtatt cttcttccgt ttgcttcttt ttttatttga tcaaaaaata
    26341 gagctaaatt ttcttggctt tgcatcaatc tatcgattgt ttctttccag agatctggtt
    26401 gttgttttat ttcttgtgtc gtgattgctg cacctatttg ttttaatttg ttcttcgata
    26461 gctcaaacat gttgttctcc ctctcaatca ttttcggata tgccgaattt tataatgaaa
    26521 ctggtccgct cttgctacac tatctgtata ttcgataatc tcatttttca cgttataggt
    26581 tatcctcgtc agatgtaaga cgggagcgcc atcagcgatt cgtagcacaa cagcatcttt
    26641 tttttgcacg atacttgcat aaaactcttc atcagctaat tgaacttgtt gttcataatc
    26701 ctgtgaaaac acatcataca acggtttact ttcaagtaac ggcaaatcaa gtcccataaa
    26761 tagtttcgcc ggtagataag aacgctcaac catcaatggc tcttcatctg ccaaacgcaa
    26821 tcttttaagt ttaaatacag tttcacctaa atggatgttc aaattcttag caattctctt
    26881 attagcttcc atttgtctaa attctaaaat gaccgtctta gggacgcgcc ctatttcgcg
    26941 catttcttct gtaaagctat atgctccagc taagttcgat gacttatcag acaaatcaga
    27001 gacataggta ccttttccat gtcttcggta aatataacct tgattttcca attcctgtaa
    27061 agccagacgt acggtggtac gactcacccc atagtgttga caaagttctc tttcagataa
    27121 cagcttatca tgcggagtca tctgtgtttc gatacgatcc ttcaataaat caactagttg
    27181 ataatacaat gattttcctt tatttgagct ctccataaaa atcacctctt ctttactggt
    27241 tataaccaca acacaagaat agtgaattat taatcatttg tcaaaggttt tttagaccaa
    27301 aaaagctatt ataacagctc tttaaagctt tatatcttga atagacaaag atacctcaaa
    27361 aataccagtt accaataaaa gaacagaaaa aaagacgaac cacataagcg atccgtcaaa
    27421 atggtctaac ttatcgagca gcgtgatcca cattaatccc tatgatattc ctacaactat
    27481 cgttttatta cttccaatat catttacaac aactaaaaat agcttattaa tgacaaatgt
    27541 tattattagt tttagataac ttttgcagtt ttatactttt tttctataaa atttgacaaa
    27601 cattttttta aataactaat tattttttga atctgtcact cagacaatcc tttctcttac
    27661 ttgttaacat aaatagaagt tgctgtttct tctaatagtg tacttaattc tttaatattt
    27721 tgttttcctt cttctaacat ccaactactc gctttttctt cgccttcggt aacaaaaggt
    27781 gctactccct tcgcccatgt tgctcgccca cataagacac cattgaatgt cgaacctgat
    27841 tgttttgcaa aacgcaatgt atcttgaaat agtgcagcgc tgactcccgc acttaaaaaa
    27901 ataaacggtt gttcagtcac tttcgattgt tcatagaaat acgcggatgc ctctgcttga
    27961 ctataacaag tatcttgccc atatccttcc acaaatctca tattcactgg tacttctact
    28021 ttcaatacat ccacttgata acgttctttt gaaaattctc gcatggcttc aatcacttta
    28081 tgcggtttta cttttgcata gtcaagagaa gacggatcgc caatagttgc atcgtaagaa
    28141 aggagctcta aaaagaaagg tatgccttct gcttcgcatt ctgaaccaat ccgttctata
    28201 actatttgtt tttgcacatt aatctctact ggttcatcaa cgtcatagta caataaaaat
    28261 ttacaagcat ccgcacccac ttcttttaac cttttcactg accaactatt cagtaaatct
    28321 ggtagtcggc ctggcatttt cgcatcatag ccggtttttt cataagctag caacaaacca
    28381 cattctttct ctcgaagcgc tgctgcaggc agaccatatt ctggatcgag taaaatagcg
    28441 gaagcatacg gagtcaggta ctgagaaacc atctgtttaa attggatgat atcttctgat
    28501 gtagcttctt tatattggtt gatcattttc ttcaaagcac cacgttggtc gatagctagc
    28561 gcgctaataa ttccagcttc atccgtcaat cgcttcaaac gttcctgttt ttctatactc
    28621 attttcttca tatatcttca cctatttctt tagttaatat tgtagctatt tctatttttg
    28681 attttgcagc tctcaactgt tctgtaacct cttttcgcat caatgatcgt gcaacgagag
    28741 acaataattt caaatgagtc gttcctgctt ctgaatctgg gataaataac gcaaaaataa
    28801 aatcaactga ctgtctatct aaactgttcc attcaattcc ttttttcagt gtgacgataa
    28861 tcaaactagg ttcattgatt gtttgagact tcgcatgagg gatagcaaaa cctgacatta
    28921 ttcctgtagt accttctttt tctctttcca gaagtttttg atatacttct ttttcgtcat
    28981 gagcgattcc taaacagaat gtttgctttg ctaaaaactt aaatgcttct tcttgattat
    29041 ctaatggctg ttgtaaaaaa atgttctctt catgaatgat tgtcatgata aacctcctgc
    29101 tcttccttct tcgataactg tacgatttgt tgctttatta tccctcttct tgtctttatc
    29161 agttactctt ttcttttgga tcaaacctag tatcactcca ccaacgatcg aaccaataag
    29221 aatcgctgta acccattgga tttttcccgt cactacaggt aacactaaaa aaccgccatg
    29281 tggggctggt acttggacac caaacagata ggtcaagaca gcagcaattg atgagcctaa
    29341 catcatcgtt ggtaatactc ggatcgggtc tttcgcagca aacggaatag ctccttccgt
    29401 aatatgagtt gatcctaaaa taaaattaac caaacctgca ttacgatcat tagtatcaaa
    29461 atatttacca aacaataaag tagcaaaacc agtaatcaac ggtggtgcaa tgcaagctgc
    29521 tgacgcacca gccataaaag tcgtgtttcc ttgtgtcaaa agagctgttc ctgttacata
    29581 tgccgcctta ttaaccggtc ctcccatatc aaacgcacac atacagccaa caatcacgcc
    29641 aagaacaagt ggattcgaat attcaaatcc agctaaaaag ctcatcattc cttcatttat
    29701 agccttcatt ggacctgcca gcaaagacat cacgatgcca ataaatgcta caccaataac
    29761 tggatataag acgattgcct tcaagccatc tagttgttta ggtaaaatta tcagtagctt
    29821 ttgcaaaaat aagataaaat aacctgctaa gaacccagct aaaatacctc ctaaaaatcc
    29881 agtaccgcca ttcatggcta cgattccacc aacaaatcca acgactaacc ctggacgttt
    29941 cgcgatccct tctgcaatat aagctgctaa aactggaacc atcattccca ttgctgcatc
    30001 gtcaactgct tttaccattg ccgctgtagc attgtattgt gcatgatctg gatcagaaga
    30061 atagattccc caaacggcaa atgaaatagc aatcaatact ccgccagcta ccacgaaagg
    30121 caacatatga gagacaccgt tcatcaaatt tttatagatg ctacgaccaa aattagcacc
    30181 acttatcgct tctggttcta aagtatcagc agcttcatta tctgctaaga cagtaccttt
    30241 tcctgccaat gcttcgtcaa tcagccgttg tgcatccttg attcctgtac tgacagaaac
    30301 atcaatgact cgttttccca caaatctttc tgaatggaca tctttgtctg cggcaataat
    30361 gacagcatct gcctgtttga tttcttcagc cgttaattca ttctcgatcc caatttgtcc
    30421 atgggtctcc actttgatcg tgatgccttt tttcctagct gcttgctcta atgcctcttg
    30481 cgccatgtag gtgtgcgcaa ttcctgtcgg acaaccggtt gctgcaatta gttgatattt
    30541 tcccatcttt cgaacctctc ttccttgatt ttgatttgtt tacttaattc ctccacatcc
    30601 aaaaaatcag tcagtccttt acgaaaagca gtagagctgc ctgctgctat gcttttacgt
    30661 aacgtttcat caagcgatcg tcgattcatg tatcctgcta aaaaagtgcc taacatggca
    30721 tcacctgcac atgctgtatt gacaactttt ccttttggcg agtttcctcg aatcaccctg
    30781 tctttcgtaa aaagaatcgc tcctttctca ccgagggata acaaaacatt ttctgcgcct
    30841 gactctacta gtcgttgtcc ataaacaaag taatcttcat ctgtaaccat ttttcgtccg
    30901 aaccaagaag aaagttcttc ctcatttggt ttcagcaaaa acggtcgata gggtaaacaa
    30961 tccatgactt cttgatcact actatcaata atcaaacaaa tccgactgat ttctattaaa
    31021 acctttggag acagccctct tggtaaactc ccagaaacac aaagataatc ccctgcttgt
    31081 aatgctctta tttgatttaa aaaattatga acagctgtct gcggtatttc aggtccttga
    31141 ttcactaatt tgtattcttc tcccgtttga ttgacttgtg taaatacatt gatacgcgtc
    31201 ataccaggca cttcaataaa ctctgtttgg atttgtttct gctgtaaata gtcttctata
    31261 tactttcctg tgaacccagc agaaaaacct aatgccgtac ttgggaaatc tagcatcttt
    31321 aatactagtg acacattcac cccttttccg tttgcctgaa tgtcatcctc ttttgtgcgg
    31381 tttaccataa aaggatgcat ctcatctgtt tcaataaata gatcaattgc caaattcatc
    31441 gtgcatgtgt agatagacat aaccaacgac ctccttgatt ctattatgaa atacgcgaaa
    31501 tagaaaatgt tttcttaatc gtcccaacac aaagaaaacc tttacattta taaactcgtc
    31561 gtgctttttt taactaactt ttggtgaaag aaggaaagaa tatgaatcac aaattaagtg
    31621 aaacagagca gtacctctgg aactttatcg aacacctcat cctagagatc cctaactatt
    31681 ccatcgtgaa actaagtgaa caagcaaatg tctcaacagc caccattgtg cggacaatga
    31741 aaaagaaagg ctatgaaggg tttacttcgt ttaagcatca tctaaaagaa ggagaaaaca
    31801 agaacatcaa tttttcattt ttagataaag tagacaaagg aatccgccga gccattctaa
    31861 aaaatgaaca agaagtggta cgtacaatca atatgttgga aatcgggaac attgaagatg
    31921 caatacaaag aatcaaattt gctgaccggg tttttgtctt tgcccgagga ttttcagaaa
    31981 tgattggcca agaaatgctg gtaaaacttc aattaacggg aaaaaattgt cagatgcata
    32041 ctgatccaga aattatcaaa agcatcagtc ggaaactaac taagaaagac agcgttattt
    32101 ttgtttcttt gaacggtgag aaaacaaata aaaacatcaa aaataagatt tatgtcttta
    32161 tttttcaatt ttttaaat
//
LOCUS       DARBET010000027        29291 bp    DNA     linear   BCT 07-NOV-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4027
            SAMEA114569841-rid20112183.denovo.027, whole genome shotgun
            sequence.
ACCESSION   DARBET010000027 DARBET010000000
VERSION     DARBET010000027.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA114569841
            Sequence Read Archive: ERR12203666
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 29291)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 29291)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001972618.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001972618.1
            Genome Coverage       :: 132x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/07/2023 13:18:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,041
            CDSs (total)                      :: 2,975
            Genes (coding)                    :: 2,908
            CDSs (with protein)               :: 2,908
            Genes (RNA)                       :: 66
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 67
            CDSs (without protein)            :: 67
            Pseudo Genes (ambiguous residues) :: 0 of 67
            Pseudo Genes (frameshifted)       :: 26 of 67
            Pseudo Genes (incomplete)         :: 28 of 67
            Pseudo Genes (internal stop)      :: 20 of 67
            Pseudo Genes (multiple problems)  :: 6 of 67
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..29291
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA114569841-rid20112183.denovo.027"
                     /isolate="VRE4027"
                     /db_xref="taxon:1352"
                     /country="Denmark"
     gene            complement(47..895)
                     /locus_tag="SCJ86_001360"
     CDS             complement(47..895)
                     /locus_tag="SCJ86_001360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MurR/RpiR family transcriptional regulator"
                     /protein_id="HEG1168931.1"
                     /translation="MSVISRIESMMEDYSSAEKKLANYIIGNIEKVPTMTANELAEAS
                     GLSAPTVVRFSKKIGFQSLTDFKITISTELQTGIDEGFSDIEANESFYSIKNKLGNNA
                     QVAIKETVDILEEETIQKVVECLESAETVFLYGVGASSLVVEDILQKWSRVGKPIIFE
                     KDIHVLLPQLVSNEKKKVLWLVSNSGRSADVVALAELAKSMNIEIIALTQFGNNPLSK
                     IADVLVQTSRPKEITNRSAATNSLLAQFATIDIIFYFYMAKNEKLSEKVKKTREAIQE
                     YFMDSK"
     gene            complement(1119..3059)
                     /gene="thrS"
                     /locus_tag="SCJ86_001361"
     CDS             complement(1119..3059)
                     /gene="thrS"
                     /locus_tag="SCJ86_001361"
                     /EC_number="6.1.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295368.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonine--tRNA ligase"
                     /protein_id="HEG1168932.1"
                     /translation="MIKITFPDGAVKEFESGTTTLAIAESISKSLAKKALAGKVNGKL
                     VDLTRPIEEDASIEIITPDHEDALGLVRHSAAHLMAQAMRRLYPNIHFGVGPAIDSGF
                     YYDTDNGQNQVTAEDLPAIEAEMMKIVKENLPIERRVLSKQEALEIFASDPYKVELIS
                     ELPEEEVITAYQQGEFIDLCRGPHVPSTGRIQVFKLLSVAGAYWRGNSNNQMMQRVYG
                     TAFFDKKALKEYIRLREEAKERDHRKLGKELDLFMVSPEVGSGLPFWLPKGATIRRTI
                     ERYIVDKEVSLGYQHVYTPIMGDVELYKTSGHWDHYQEDMFPPMDMGDGEMLVLRPMN
                     CPHHMMVYKNTIHSYRELPIRIAELGMMHRYEKSGALSGLQRVREMTLNDGHTFVRPD
                     QIKDEFKRTLELMVAVYADFNITDYRFRLSYRDPNNTDKYFDDDAMWEKAQAMLKAAM
                     DELELDYFEAEGEAAFYGPKLDVQVKTALGTEETLSTIQLDFLLPERFDLTYVGEDGE
                     NTHRPVVIHRGIVSTMERFVAYLTEVYKGAFPTWLAPIQATIIPVSVDAHGDYAYEIK
                     ERLQMKGLRVEVDDRNEKMGYKIRASQTQKIPYQLVVGDKELEDATVNVRRYGSKETA
                     VEDLNIFIDAMEAEVKNYSREN"
     gene            complement(3396..3575)
                     /locus_tag="SCJ86_001362"
     CDS             complement(3396..3575)
                     /locus_tag="SCJ86_001362"
                     /EC_number="5.3.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016252354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-oxalocrotonate tautomerase"
                     /protein_id="HEG1168933.1"
                     /translation="MPFVHVELVEGRSPEQLENMMKDITEAVHKNTQAPKEHIHVIIN
                     EMKKGTYGVNGEWKK"
     gene            complement(3622..5445)
                     /gene="cadA"
                     /locus_tag="SCJ86_001363"
     CDS             complement(3622..5445)
                     /gene="cadA"
                     /locus_tag="SCJ86_001363"
                     /EC_number="7.2.2.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324385.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cadmium-translocating P-type ATPase"
                     /protein_id="HEG1168934.1"
                     /translation="MTHFKKFLLTLLIGGIALLSEFVFHQPALAFAIVAFTGGILAFL
                     MFLEMIKTLRSGRYGVDVLAITAILATLAVKEYWASLMILIMLTGGDSLEDYANKRAG
                     KELQSLLDNTPRIAHRFKEGKLQDIVIDEINIDDQLLVKPGELVPADGRVTKGESSVD
                     ESSLTGESRPETKKPGDQIMSGSLNGDGSIQMIAEKRAADSQYQMIIKLVEESKEKPA
                     RFVRLADRYAVPFTLIAYLIGGISWWFSKDPLRFAEVLVVASPCPLILAAPIALVAGM
                     SRSSKNGIVVKTGTTIEKLARTKTIAFDKTGTLTKGILEVEAIQPVNGIKKEELLLYA
                     ASAEQESSHILARSLVNNVKNSQLYPVSQLKEVTGQGIQAVVNNHEVKIGRASFVSAE
                     EIETSNTAIYVTIDRSFAGTILFSDQIRPEAAKTIKELRKLDVKNLMMITGDGSAIAQ
                     TIAEKVQLDSVHARSLPQDKLTILESIPKDQRPVAMVGDGVNDAPALAAADIGIAMGA
                     HGSTAASESADAVILKDDLLRVAKAVVISRDTMRIAKQSVLIGIFICIFLMLIASTGL
                     IPALFGAVLQEVVDTVSILSALRAKKDPAIFPDSVPKTNIV"
     gene            5723..8002
                     /locus_tag="SCJ86_001364"
     CDS             5723..8002
                     /locus_tag="SCJ86_001364"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HEG1168935.1"
                     /translation="MNEKQLEQTHVDETIELIQIEQNMLDTKQQELTQEMNHSTKDTA
                     NHKIRAGSNEAFYESAVEYRQHEQELLLKYHTLEAQQKRLQTLSVMKDSPYFARIDFK
                     EDRETETLYLGIASLRDLAEETIVIDWRAPIANLYYEGEIGPAFYETETDRIDVDLQL
                     KRQFKIQEGRILSMVDTSEVINDDFLLEILDESSSNHMKNIVSTIQKAQNAIIRDTTS
                     KVMLIEGIAGSGKTSALLQRIAFLLYHNRKWLEAENVLLFSPNHLFSDYISTVLPSLG
                     ESGVPTQTFKNYLVQLLPEFSLLDEQQQESGFLLGEKDPIQVMKSGLTLVDQIDRYIQ
                     GITNYGPLFRDMKINGRTILSKESIRQWYKETNEQLPLHHRLSLLQTKLLKKLGGLQK
                     DETRQQWVKDLAEEQLQELYATDPNLEYTEQKERSLRKKLANQIVRKRFRRIHRGIMN
                     YQFVNITKQYLHFLQTVPLSLLTRHKISETRWQEHLTTVRTHLRQRELRQEDAVLFFL
                     LMRRTYPVTVTHKARYIFIDEMQDFAPSQVALLQALFPKANLTFCGDLNQNVFGNETI
                     VGSLEDLFVDREVTRFQLTTSYRSTKEITDFANHFIVKEHQVETTARKGDLPLLIQGT
                     TDDIKLNWLADTIRTTQGKARYWRTAIIAKTQEECEALYAQLPDELKQQVQLILNESD
                     FMKRSIILLPAFLAKGLEFDRVFLWDVDAEHFSSQQDKLILYTMCTRAMHELTLFTSN
                     NESPLLNNMDISSYEKKVL"
     gene            complement(8095..8742)
                     /locus_tag="SCJ86_001365"
     CDS             complement(8095..8742)
                     /locus_tag="SCJ86_001365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341283.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEG1168936.1"
                     /translation="MEAKITLEPFERILSGYRKVEELAVNVTDCSKLAQKYARFGVEG
                     YRLGNYVGTGYLNRYLECMVDRAPMLIYRQNYLIPLLFRRSDSAFRLFEEEYRMEAFF
                     LLLEWSLKHRPEKILIERNEKIDTKKNKVIDSAYLAFRVSEILDCGGYPISNFQSIDQ
                     FIEWNRIYRLIDNGGIGRHSKVFDPEYPENMEELKMIISLVKLKYPETDLDLYIE"
     gene            complement(8921..9640)
                     /locus_tag="SCJ86_001366"
     CDS             complement(8921..9640)
                     /locus_tag="SCJ86_001366"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373480.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YebC/PmpR family DNA-binding transcriptional
                     regulator"
                     /protein_id="HEG1168937.1"
                     /translation="MGRKWANIVAKKTAKDANNSRIYAKFGIEIYAAAKSGDPDPHAN
                     QKLRFVIDRAKTYNVPKHIIDRAIEKAKGAGDETYSELRYEGFGPSGSMVIVDTLTNN
                     VNRTAADVRAAFGKNGGNMGVSGAVSYMFDNTAIFAFAGEDADEILEYLMEKDIDVRD
                     VMEEEGQIIVYGEVADFHSIQEALKEKGITDFSIAEIQMIPQNEVTLSPEDQETFEKM
                     IDALEDLEDVQHVFHNVALED"
     gene            complement(9771..10922)
                     /locus_tag="SCJ86_001367"
     CDS             complement(9771..10922)
                     /locus_tag="SCJ86_001367"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298249.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyltransferase"
                     /protein_id="HEG1168938.1"
                     /translation="MKSCGIIVEYNPFHNGHRYHVEMARKITGAEVVIAVMSGNFLQR
                     GEPAIIDKWHRANEALQNGVDLVVELPVEWAVQSADYFAKGAVKILQTLDCDSLCFGT
                     EEETSFDYSSFGRFVQENQDRIEEAFQELSDQTYSYPQKMTEVFRSLYPDITLDFSSP
                     NHILGLSYAKENAKYVHPMELHPIRRKSAGYHEKIITDQRIASATAIRHALEEGKRIA
                     GLVPPQTEEDLSTCSLQTWESYWPYLRYRLLSSTLLELTDIYQMTEGIESRMKTAAAT
                     ADSFEDFVSKVKTKRYTWTRIQRLSCYVLLNIKKQEIPVQQNKNYLRLLGFTDAGRRF
                     LKEKKEISIYSRIGKKEAQEAELLVRSDQIYQLGGNIPEQNFGQIPIRV"
     gene            complement(11139..11879)
                     /locus_tag="SCJ86_001368"
     CDS             complement(11139..11879)
                     /locus_tag="SCJ86_001368"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HEG1168939.1"
                     /translation="MAYETFAFIYDEVMDETLYQQWLAFSKRHLQEDTKNILELACGT
                     GALAVAFAKDGYKVTGLDLSEEMLMIASQRAYEEDASIQFVEGNMLDLSEIGQYQAIT
                     CFSDSLCYMKNRQEVQQVFDEVYQALEENGRFLFDVHSTYQVDEVFPEYSYHYQGDNF
                     AFLWDSYPGSEKHSIEHFLTFFVEEQEGSGRFIREDELHQERTYPLESYLRMLENSGF
                     SKVEAFSDFTDEAASEKSKRWFFVAYKD"
     gene            complement(11883..12206)
                     /gene="rsfS"
                     /locus_tag="SCJ86_001369"
     CDS             complement(11883..12206)
                     /gene="rsfS"
                     /locus_tag="SCJ86_001369"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010719657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome silencing factor"
                     /protein_id="HEG1168940.1"
                     /translation="MEAADSKRAEDIIALDVRNVSLLADYFMICSANSERQINAITEE
                     IIDKEEENKYEVKRIEGKEGGKWVLIDLGDVIVHVFHAPERNFYNLEKLWSDAPLVDL
                     SEWLD"
     gene            complement(12263..12856)
                     /gene="yqeK"
                     /locus_tag="SCJ86_001370"
     CDS             complement(12263..12856)
                     /gene="yqeK"
                     /locus_tag="SCJ86_001370"
                     /EC_number="3.6.1.41"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326076.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bis(5'-nucleosyl)-tetraphosphatase (symmetrical)
                     YqeK"
                     /protein_id="HEG1168941.1"
                     /translation="MNMDYSNEYTAYSRENLMQKIQMRMSEQRFKHVLGVEETAVALA
                     KKYGASPEKASIAALTHDYAKERPDEEFKMVIVRDGFDPELLNYNNAIWHGLVGASFV
                     ERELGITDAEILHAIRVHTTGAAKMSLLDKIIYVADYIEPGRDFPGVQDARAIAWADL
                     DEAVAFETKHTLAHLLAQEQQIYPKTIETYNYWVAKK"
     gene            complement(12840..13490)
                     /locus_tag="SCJ86_001371"
     CDS             complement(12840..13490)
                     /locus_tag="SCJ86_001371"
                     /EC_number="2.7.7.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287632.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinate-nucleotide adenylyltransferase"
                     /protein_id="HEG1168942.1"
                     /translation="MMNTQAKTFVRSQLFPEEMPQFLEKKKQVGILGGTFNPVHLAHL
                     VMAEQAGRNLGLDRVFLMPSYQPPHVDEKQTIDAKHRLNMLELAVEDNPFLQIETIEL
                     ARGGKSYTYDTMKELTQNNPDTDYYFIIGGDMVEYLPKWYKIDELTSMVNFVGIRRPG
                     YTTDTPYPVIWVDVPEIDISSTKIRQKIKEGCSIRYLVPDKVIDYIQNEGLYEYGL"
     gene            complement(13500..13811)
                     /gene="yhbY"
                     /locus_tag="SCJ86_001372"
     CDS             complement(13500..13811)
                     /gene="yhbY"
                     /locus_tag="SCJ86_001372"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016632008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome assembly RNA-binding protein YhbY"
                     /protein_id="HEG1168943.1"
                     /translation="MSLRGKQKRFLRSKAHHLQPIFQIGKGGINSAMIVQIEEALEKR
                     ELIKVSLLQNTDEIAEEAAQVLEKEIDCEIVQIIGRVIVLYKPSTKEKYQRLSKEVKA
                     I"
     gene            complement(13827..14936)
                     /gene="yqeH"
                     /locus_tag="SCJ86_001373"
     CDS             complement(13827..14936)
                     /gene="yqeH"
                     /locus_tag="SCJ86_001373"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002370701.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome biogenesis GTPase YqeH"
                     /protein_id="HEG1168944.1"
                     /translation="MTEELQCIGCGAVIQTNKPGELGYTPQTALEKGLASGEVYCQRC
                     FRLRHYNETQDVSLTDDDFLRLLNELGQKDALIVNVVDIFDFNGSLIPGLHRFVGDNP
                     VLLVGNKVDILPKSLKKTKLTQWMKERAYEAGIRPVDVLLTSAKKSHEMTELLEKIEE
                     YRDGRDVYIVGVTNVGKSTLINQIIKNTAGVQDVITTSQFPGTTLDKIEIPLDDGHFL
                     IDTPGIIHRHQMAHYLGKKDLRIIAPLKEIKPKVYQLNEGQTLFLGGLARFDFISGNR
                     ASFIAYVSNDVNIHRTKLEKADEFYQKHVGGLLQPPRPDEVEAFPELVRFEFSVKEKT
                     DIVFAGLGWITVTAPVVIAGWAPKGVDVLKRKALI"
     gene            complement(14933..15460)
                     /locus_tag="SCJ86_001374"
     CDS             complement(14933..15460)
                     /locus_tag="SCJ86_001374"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853310.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YqeG family HAD IIIA-type phosphatase"
                     /protein_id="HEG1168945.1"
                     /translation="MFSNYKPTWMVEAIYQITPAQLKNLGIKAVLTDLDNTLIAWNNP
                     DGTEELLTWILEMKNAGIPVVVVSNNNSKRVARAIEKFDLLYVARAMKPLARGINIAK
                     KQLDLADDEIVMVGDQIMTDIRGANRAGIRSILVKPIIATDSWKTQFNRFWEKRIMAY
                     LLAKHPDMGWRGGIE"
     gene            complement(15640..16425)
                     /locus_tag="SCJ86_001375"
     CDS             complement(15640..16425)
                     /locus_tag="SCJ86_001375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343529.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase carboxyl transferase
                     subunit alpha"
                     /protein_id="HEG1168946.1"
                     /translation="MDKTAHDVVQLARAKERWTSLDFFEEIFDSFEECHGDRLFADDE
                     AIVGGIARLDGKPVTVIGIQKGRDLQENIRRNFGSPHPEGYRKALRLMKQAEKFNRPV
                     ITLINTAGAYCGVGAEERGEGEAIARNLLEMSDLKVPIIAIIIGEGGSGGALALGLAD
                     EVWMLEETIYAVLSPEGFASILWKDGSRAAEAAELMKITAPELRELGIVDRVIPETFN
                     GEQLPRYKVAHIMKKALIERLTELVRLDQHKLLEKRYKRFRQY"
     gene            complement(16418..17287)
                     /locus_tag="SCJ86_001376"
     CDS             complement(16418..17287)
                     /locus_tag="SCJ86_001376"
                     /EC_number="6.4.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290846.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase carboxyltransferase
                     subunit beta"
                     /protein_id="HEG1168947.1"
                     /translation="MALFKKKKYIRINPNRESKPANSPSVPDNMWAKCPNCKHILYTK
                     DIGEEKVCPHCGYAFRIGAWQRLALIIDEKSFEEWDTDLVTKDPLAFPEYTEKIKKMQ
                     DKTGLHEAVLTGKATIQGIPFIIGVMDPNFIMGSMGTIVGEKITRLFERATKESLPVV
                     LFTASGGARMQEGIFSLMQMAKISAAVKRHSNEGLFYLTVLTDPTTGGVTASFAMEGD
                     IILAEPQSLIGFAGRRVIEQTIKQELPEDFQKAEFLLSHGFVDQIVPRMELKQKIHTL
                     LELHTQKGWIDRG"
     gene            complement(17288..18667)
                     /locus_tag="SCJ86_001377"
     CDS             complement(17288..18667)
                     /locus_tag="SCJ86_001377"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase biotin carboxylase
                     subunit"
                     /protein_id="HEG1168948.1"
                     /translation="MFSKVLIANRGEIAVRIIRACRELGIQTVAIYSEADAEALHTQL
                     ADEAICIGPAKAVDSYLNVQEVLSAAIVTKAEAIHPGFGFLSENSRFASMCEECNITF
                     IGPKSATIDAMGNKINARQLMQEANVPVIPGSTGVLSTVEEALEIADRIGYPVMLKAA
                     AGGGGKGIRKVQSKEELPQHFSSAQQEAAAAFGNDDMYLEKIIYPARHIEVQILGDRF
                     GHVIHLGERDCSLQRNNQKVLEESPSVVISQTKREALGDAAVRAAKAVNYENAGTIEF
                     LMDEEGDFYFMEMNTRIQVEHPVTEMVTGIDLVKKQVEIAAGEPLNVCQEEVVFQGHA
                     IECRINAENPAFHFAPSPGKIQNLLLPAGGMGLRVDSAVYPGYTIPPYYDSMIAKIIV
                     HGNTRFEALMKMQRALSEFITEGIITNAEFQMDLISHPAVIAGDYSTAFLQEEFLPNW
                     TPETEIGGA"
     gene            complement(18675..19094)
                     /gene="fabZ"
                     /locus_tag="SCJ86_001378"
     CDS             complement(18675..19094)
                     /gene="fabZ"
                     /locus_tag="SCJ86_001378"
                     /EC_number="4.2.1.59"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723308.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-hydroxyacyl-ACP dehydratase FabZ"
                     /protein_id="HEG1168949.1"
                     /translation="MNIQEIKEIIPHRYPMLLIDRVEEMVEGERIVAKKNVTINEPFF
                     QGHFPEEPVMPGVLIVEAMAQAGAVALLSLEQFKGKTAYFGGLDKAKFRKKVTPGDTL
                     YLEVEILKVKASAGIGKGIAKVDGKKVAEAELTFMIG"
     gene            complement(19119..19595)
                     /gene="accB"
                     /locus_tag="SCJ86_001379"
     CDS             complement(19119..19595)
                     /gene="accB"
                     /locus_tag="SCJ86_001379"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302926.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase biotin carboxyl carrier
                     protein"
                     /protein_id="HEG1168950.1"
                     /translation="MDINEIRELVSQFDQSSLTEFDLREGQFELYMNKNKTSRGMNMV
                     QTNETLPVSEPVNVIPEQATTAPEIMKEKESAVFDGIEVVSPIVGIVYLQPAPDKPAF
                     KKVGDQVAKGEVICIIEAMKLMNEITSEADGVITEILVENESVVEYGQPLFRIQQK"
     gene            complement(19598..20833)
                     /gene="fabF"
                     /locus_tag="SCJ86_001380"
     CDS             complement(19598..20833)
                     /gene="fabF"
                     /locus_tag="SCJ86_001380"
                     /EC_number="2.3.1.179"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735874.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-ketoacyl-ACP synthase II"
                     /protein_id="HEG1168951.1"
                     /translation="MNRVVITGYGVTSPIGNDADSFLNSLKEGTNGIAPITKFDASET
                     GITLAGEVKDFPYDKYFIKKDSKRMDMFSIYGIYAALEAMEMSGLDKEKMNQDRFGVI
                     IGSGIGGLPTIENQVIRLHEKGAKRVSPMFVPMAIANMAAGNIALRVGAKGICTAIVT
                     ACASGTNSIGEAFRNIKHGYSDVILAGGTEATICEMGIAGFAALTALSESTDPNRGSI
                     PFDENRNGFVMGEGAGVLVLESLEHAQARGAKIYGEIVGYGANCDAYHMTAPTPDGSG
                     ASKAMKLAMEEAGVQPEQVGYINAHGTSTPANDQAEAKAIQIALGENFRDTYVSSTKS
                     MTGHLLGAAGGIEALATLLALEHQFIPPTINVEKQDPEIDLTVVKNESKPASFTYAMS
                     NSLGFGGHNAVLCMKRWEE"
     gene            complement(20852..21589)
                     /gene="fabG"
                     /locus_tag="SCJ86_001381"
     CDS             complement(20852..21589)
                     /gene="fabG"
                     /locus_tag="SCJ86_001381"
                     /EC_number="1.1.1.100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324391.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-oxoacyl-[acyl-carrier-protein] reductase"
                     /protein_id="HEG1168952.1"
                     /translation="MDVKGKNVFITGSTRGIGKAMALAFAKAGANIILNGRGEIPKEK
                     IEEIEAFGVKCVGVSGDISDYEKAGQMIKEAEEKLGSIHVLVNNAGITNDKLVMRMDA
                     EDFKKCLDINLIGTFNMTQHVLKKMMKQREGAIINLSSVSGLIGNIGQANYAASKAGV
                     VGLTKSVAREAATRGITCNAIAPGFITTDMTEVLADKVKEQAEKQIPMQRFGQVEDIA
                     QTAVFLAQNPYITGQVINVDGGLVMHG"
     gene            complement(21601..22518)
                     /gene="fabD"
                     /locus_tag="SCJ86_001382"
     CDS             complement(21601..22518)
                     /gene="fabD"
                     /locus_tag="SCJ86_001382"
                     /EC_number="2.3.1.39"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339663.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ACP S-malonyltransferase"
                     /protein_id="HEG1168953.1"
                     /translation="MKTAFLFSGQGAQYQGMGKDLYEEAVVKQTFDEASEILGYDMAE
                     LCFTENERLDQTQYTQPAILTVSIAYYRLLKEHGIIPDAALGLSLGEYSALVASNALS
                     FTEAVALVAKRGAYMTEAAPAGSGKMVAVMNAPIETIEESCHEASKYGIVSPANYNTP
                     QQIVIGGEEKAVDEAVLLLKEKGFKRMIPLNVSGPFHTAILEPAAKKLAKDLSQIQFS
                     EPSFPIISNTTTEIMKKETIAGLLEQQVMQPVRFYESIHKLKTIGIEQVIEVGPGKVL
                     SGFMKKIDKTIPVLRVENKQTFDETIAIL"
     gene            complement(22625..22849)
                     /locus_tag="SCJ86_001383"
     CDS             complement(22625..22849)
                     /locus_tag="SCJ86_001383"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344088.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl carrier protein"
                     /protein_id="HEG1168954.1"
                     /translation="MVFEKIQEIIAEELGKETEEIKLTTDIQEDLEADSLDLFQIINE
                     IEDEFDVKIETEDGIKTVQDLVTYVEKQTA"
     gene            complement(22902..23864)
                     /locus_tag="SCJ86_001384"
     CDS             complement(22902..23864)
                     /locus_tag="SCJ86_001384"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738756.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ketoacyl-ACP synthase III"
                     /protein_id="HEG1168955.1"
                     /translation="MEQFGMITATASVLPEKIITNNDLSKMMDTSDEWISSRTGIRER
                     RCVTNQETSDLCILVAEKLIKQKNLNPKDLDFILVATMSPDYTTPSVAAQVQGKLGAT
                     HAIAFDISAACSGFVYALSMAEKMIRCGSTKGLVIGGETLSKLIDWSDRTTAVLFGDG
                     AGGILLEANPQKKLLKEKLAADGTRSSSLTAGYQQNKNPFYSEDSEGSYYLQMTGRDI
                     FDFAVRDVANNIQEVMKDKPVDYLLLHQANLRIIEKIARKVKMPQEKFLTNMDKYGNT
                     SAASIPILLDEAVSSGKITLGKQQKVIFTGYGGGLTWGSILMEL"
     gene            complement(23874..24314)
                     /locus_tag="SCJ86_001385"
     CDS             complement(23874..24314)
                     /locus_tag="SCJ86_001385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005471259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HEG1168956.1"
                     /translation="MEPDLKTVNDYLVSVFNDILTIEESELKKSQFKDLSITEMHTIE
                     AIGMYKKKTTSEVAKELSITVGTLTTAINKLVKKGYVERIRSEDDRRVVKLGLTKKGK
                     LLFRVHQHFHREMVKNILDGMATEEQHALLDALKNLHDFLQDYK"
     gene            24620..25585
                     /locus_tag="SCJ86_001386"
     CDS             24620..25585
                     /locus_tag="SCJ86_001386"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GIY-YIG nuclease family protein"
                     /protein_id="HEG1168957.1"
                     /translation="MLKEKMKTLPMLPGVYLMKDRDGKVIYVGKAKRLKNRVSSYFHQ
                     NKQHSKKVLRMIHHITDFDFVVVDTELDALLLECQLIQHYRPFYNRQMNYFSNYNYVH
                     ITNKGFVLTDTPTARTYGPFRLYKKMPSILRIMEETYQMPWLSEISLLALRVQLPDLQ
                     EMTFEQKKKELQGLFQGRNKKLLTYLKKRQQHFIYQLNFEKAGMLQKDIELVTYFIRR
                     IQEQKQFLRTPSLTFSMPLAADESQKKHYLVCYGQLAETMITSGDVSPDFYYEKKEAH
                     LSLKRQLSKEEIDPVQILISYRKKLEKEQFEIELLNKKEAEKQLN"
     gene            complement(25642..25872)
                     /locus_tag="SCJ86_001387"
     CDS             complement(25642..25872)
                     /locus_tag="SCJ86_001387"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YkuJ family protein"
                     /protein_id="HEG1168958.1"
                     /translation="MTHSQLVAIIKRLEAMVESADNELQVRRFEKEGVEKCTVTYDKA
                     TDTFELTETDTHQQYEFDNIDIVAMEIYDLIQ"
     gene            complement(25958..26845)
                     /locus_tag="SCJ86_001388"
     CDS             complement(25958..26845)
                     /locus_tag="SCJ86_001388"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301386.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-dependent oxidoreductase"
                     /protein_id="HEG1168959.1"
                     /translation="MKLGFIGTGVMGSAVARHLLEAGHEVAVYNRTKAKADPLVTEGA
                     IWADTPKAVAEQSNILFTMVGYPKDVEEIYYGQSGIFSADISGHILVDLTTSTPSLAE
                     KIAKTAKEKGADALDAPVSGGDLGAKNGTLTIMVGGEEAVYDQVLPLFKEFGTTFTLH
                     GSAGKGQHTKMANQIMIAGTMTGMTEMLVYAQKNGLDLKKVIETLSGGSAANWSLSNY
                     SPRILKEDYTPGFFVKHFIKDLKIALEEAEKMDLVLPATTQALKLYEELADKGFENDG
                     TQALIKLWWPEGKIPEKKS"
     gene            complement(26934..28157)
                     /locus_tag="SCJ86_001389"
     CDS             complement(26934..28157)
                     /locus_tag="SCJ86_001389"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010706866.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HEG1168960.1"
                     /translation="MKIGFFTDTYFPQVSGVATSIKTLKQELERHGHEVYIFTTTDPN
                     ADKLEKDVIRMPSVPFISFKDRRVVVRGMWYAYLVAKELELDLIHTHTEFGAGILGKM
                     VAKKLKIPLIHTYHTMYEDYLHYIGKGKVIRQSHVKYLSRLFANHTTGVVCPSERVID
                     TLRSYGVIAPLRVIPTGIDIEKFKRSDITQQDISKLRESLGLQENHLMILSLSRISYE
                     KNIQALINGFPQVISAYPNARLVIVGKGPYVEELEELISELQLEEFVQFTGEVDNNDV
                     ALYYKAADYFVSASTSETQGLTYTEAMAAGTPCVVEGNAYLNRLFDHPSLGKTFETDE
                     DFAPALIDYISSEVPKDPKVLEAKLYDISSTHFGEAMIEFYQDMVVYHEEEMRRKEEE
                     DSIERIKIKLNSFKR"
     gene            complement(28174..29220)
                     /locus_tag="SCJ86_001390"
     CDS             complement(28174..29220)
                     /locus_tag="SCJ86_001390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323526.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HEG1168961.1"
                     /translation="MKVLLYFESEKMLAKSGIGRALDHQKRALTEVGISYTLDEKEDY
                     DILHINTYGINSHNMVNKARREGKKVVYHAHSTEEDFRNSFIGSNQLSPIVKKYLVGL
                     YQKADYLITPTPYSKQLLESYGIRVPIQAISNGIDLEKYHPDPMKEQKFREYFKLSPD
                     QKVIICVGLFFERKGIIDFVEIAKKMPEYTFIWFGHVPMYSIPRNIRKIVKEDHPENV
                     LFPGYIRGEIIEGAYSGADLFFFPSYEETEGIVVLEALASKQNVLVRDIPVYNGWLED
                     SKNCYMGKTNEEFIRLIQQITNQELPSTTQAGYQTAKERSINKIGEELKNVYESVLNE
                     KVSSKLKKVNQVKD"
BASE COUNT         8115 a         6417 c         5049 g         9710 t
ORIGIN      
        1 tacgttttgt tattctaaaa aacttatata aaaaatagta aggtttttat ttcgaatcca
       61 taaaatattc ttggattgct tctcgtgttt ttttcacttt ttcgctaagc ttttcgtttt
      121 tagccatata gaaataaaaa atgatatcga tcgtagcaaa ttgggccaaa agtgaattag
      181 ttgccgcact tcgattcgtg atttcttttg gtctagatgt ttgtacgagt acatcggcta
      241 tcttgctaag cgggttattt ccaaattgcg tcaacgcaat gatttcgata ttcatgcttt
      301 tcgccagctc ggctaatgcg actacatctg cacttctacc tgagtttgaa acaagccaaa
      361 gcactttctt tttctcattg ctgacaagtt gcgggagcaa gacgtggata tctttttcaa
      421 aaatgattgg cttaccgaca cgagaccatt tttgcagaat atcttctact actaaagaag
      481 aggcgccaac cccatataaa aaaacagtct ctgccgattc caaacactct acgacttttt
      541 ggatcgtttc ttcttctaga atatctactg tttcttttat cgctacttgt gcattgttac
      601 caagtttgtt tttgatagag taaaaggact catttgcttc tatatcacta aaaccttcat
      661 cgattccagt ctgtagttct gttgaaatcg tgattttgaa gtccgtcaga ctttgaaaac
      721 cgattttttt ggaaaaacgt acaactgtcg gcgcactcaa cccggaagct tctgctaatt
      781 cattagctgt cattgtaggg accttctcaa tattaccgat aatgtagttg gccaactttt
      841 tttctgctga agaataatct tccatcatac tttcaatacg actaattacg ctcataagtt
      901 cctccaccct tttaatgata gtctatgtaa gtataccata tcagaccctt attcacggtc
      961 gtatactgga taaaaaacaa aaaaacaatc cgcccaacag aatatcttat cccatcaatc
     1021 ccacagattc tattgagcat ctgtgtcttt gaggaatacc tgttatctat tgttcggatt
     1081 gctttctatc tactactttt ttacaggaaa tttacctatt agttttctct gctgtaattt
     1141 ttgacttctg cttccatagc atcaataaag atattcaaat cttctacagc tgtttcttta
     1201 cttccataac gacgtacgtt cactgttgca tcttccaatt ccttgtctcc aacaactaat
     1261 tggtaaggga tcttttgtgt ttgagaggca cgtatcttat agcccatttt ttcattacga
     1321 tcatcaactt ctacacgcaa ccccttcatt tgcaaacgtt ctttgatttc ataagcataa
     1381 tcgccatgtg catcaacaga aacagggatg attgttgctt gtataggtgc caaccatgtt
     1441 ggaaaagcgc ctttgtatac ttctgttaaa taagcgacaa aacgttccat tgtagaaaca
     1501 attccgcggt ggataactac tggacgatgc gtattttccc cgtcttctcc aacatatgtc
     1561 aaatcaaagc gctctggcaa taagaagtct aattgtatag ttgataatgt ttcttctgta
     1621 ccaagagctg ttttgacttg aacatctagt tttggtccgt aaaaggctgc ctcgccttct
     1681 gcttcaaagt aatctagttc taattcatcc atagcggctt tcagcattgc ttgggctttt
     1741 tcccacattg cgtcatcatc aaaatattta tctgtattgt ttggatcgcg atagctcaag
     1801 cggaaacgat aatctgtaat attgaagtca gcatatactg ccaccatcaa ttcaagcgta
     1861 cgtttaaatt catctttgat ttgatcagga cgaacaaatg tatgaccatc attcaacgtc
     1921 atttctcgta cacgttgtaa acctgacaaa gcacctgatt tttcatagcg atgcatcatt
     1981 cctaattcag cgatacgaat tggtaattca cgataagaat ggatcgtgtt tttgtagacc
     2041 atcatgtgat gagggcagtt cattgggcga agaacaagca tttcgccatc tcccatatcc
     2101 attggcggga acatgtcttc ttgataatga tcccagtgtc cagatgtttt gtacaactca
     2161 acatctccca ttattggtgt atatacatgt tggtaaccta gactaacttc tttgtctacg
     2221 atataacgtt cgatcgtgcg tcggattgtt gccccttttg gaagccagaa tggcaaacca
     2281 gaacctactt ctggggaaac catgaacaag tcaagttctt ttcctagttt acgatggtct
     2341 ctttcttttg cttcttcacg gagacggata tattctttca aagctttctt gtcaaaaaag
     2401 gctgtcccgt atacacgttg catcatttga ttgtttgagt ttccacgcca ataagcacct
     2461 gccactgata ataatttaaa tacttgtata cggccagtgg atggaacatg tggtccacga
     2521 cacaaatcga tgaattcgcc ttgttgataa gcagtaatca cttcttcttc tggcaattca
     2581 ctaatcagtt ctactttgta cggatcactt gcaaaaattt ctaatgcttc ctgttttgaa
     2641 agaacacggc gttcgatcgg cagattttct ttgacgattt tcatcatttc tgcttcgatt
     2701 gccggcaaat cttctgcagt tacttggttt tgtccattat ctgtatcgta gtaaaaccct
     2761 gaatcgatag ctggtccaac gccaaagtgg atatttggat agaggcgacg catggcttgg
     2821 gccatcaaat gagctgctga atgacggact aagccaagtg catcttcatg gtctggtgtg
     2881 atgatctcaa tacttgcatc ttcctcgatt ggacgagtca aatcaacgag ttttccgttc
     2941 actttaccag ctaaagcttt ttttgctaag ctttttgaaa tgctttcagc aattgccaat
     3001 gttgttgttc cagactcaaa ttctttgact gcaccatctg gaaaagtaat ttttatcata
     3061 atagtttcct ccttgaaatg ttttgattga tcagatcatc aagctgaaaa acaaaaaaat
     3121 cctagcatct cttgaaaaga gatactagga cgaaaagttc gtggttccac ctaatgttca
     3181 aacgcaaatg cgttcctcaa gcgttgtaac ggtccgaccg cccttgcttc aacaagggtt
     3241 tcgaaagtgg tcatcctttg gttttctcct ggaaaagttt cagcctagct tttcctctct
     3301 tatcgaagac tctcaaattc agttggcttt ctcatcaatc aattatgtgt ttatttaacc
     3361 actaataaat agaaaaatca agtgggaaac taaacttatt ttttccattc cccatttaca
     3421 ccgtatgttc cttttttcat ctcattgatg atgacatgga tgtgttcttt tggtgcctgc
     3481 gtgtttttat gaacagcttc cgtgatatct ttcatcatat tttctaattg ttccggactg
     3541 cgaccttcta ctaattcgac atgtacgaat ggcataatgc tctctcctct ttctgatcgt
     3601 tcttttttca actcgattct atcatactat gtttgtcttg ggtactgaat caggaaaaat
     3661 ggctggatct ttttttgctc tcaaggcact caaaatagag accgtatcca cgacttcttg
     3721 caaaacagct ccgaataatg cggggattag tcccgtactt gcaattaaca tcaaaaaaat
     3781 acaaataaag atgccaatca gtacagactg tttcgctatt ctcatcgtat ctcttgaaat
     3841 cactactgct tttgctacac gcaagagatc atctttcaag atgaccgcat ctgcactttc
     3901 actagcagct gtagaaccat gagcccccat tgcaataccg atatctgcag cagcaagtgc
     3961 tggtgcgtca ttcacgccgt ctcctaccat cgcaacaggt ctttgatctt tagggatact
     4021 ttctaaaatg gtcagtttat cttgaggcaa gctacgggca tgtacagaat ctaattggac
     4081 tttttctgca atcgtttgag caatggcaga tccatccccg gtgatcatca tcaaattttt
     4141 cacatcaagt tttcttagct ccttgattgt ctttgctgct tctgggcgaa tctgatccga
     4201 aaataggatc gttcctgcaa aagatcgatc aattgttaca tagattgctg tattggacgt
     4261 ttctatttct tcagctgaaa caaaagaggc acgtccaatc tttacttcat gattattgac
     4321 tactgcttgg attccttgac cggtcacttc tttcagttga ctaacaggat acagttggct
     4381 gtttttcacg ttgttcacta acgaacgagc taagatatgg ctagattcct gttctgcact
     4441 agctgcatac agaagtagct cttctttttt tattccattg actggctgaa tcgcctctac
     4501 ttccaatatc cctttggtaa gcgttcctgt tttatcaaaa gcaatcgttt ttgtacgtgc
     4561 gagcttttca atcgttgttc cagtttttac aacgatcccg tttttacttg aacggctcat
     4621 tcctgcaact aaagcaatgg gcgcagctag aatcagtgga caaggtgatg ccactaccag
     4681 tacctcagca aatcgtaaag ggtcttttga gaaccaccaa gaaataccac cgatcaaata
     4741 agcaatcaac gtgaaaggga ctgcataacg atcagctaaa cggacaaatc gggcaggctt
     4801 ttcttttgat tcttcgacta gtttaataat catttgatac tggctgtctg cggcacgttt
     4861 ttcagcgatc atttggatcg atccgtctcc atttaaagac cctgacataa tctgatctcc
     4921 aggtttcttg gtttccgggc gggactcacc tgtcaaagaa gattcatcga ccgaagattc
     4981 tcctttggtc actcgaccat ctgcgggtac aagttcgcca ggcttgacta gtaactggtc
     5041 atcaatattt atttcatcga ttactatatc ctgcaatttt ccttctttga atcgatgggc
     5101 aattcttggg gtgttatcta acaaagactg cagttcttta cccgctcgtt tattcgcgta
     5161 atcctctaga ctatctccgc ccgtaagcat gatcaatatc atcaaactag cccagtattc
     5221 tttgacggct aatgttgcaa ggattgcggt aatcgctaat acatctacgc catatcttcc
     5281 tgaacgtaac gtcttgatca tttccaaaaa catcaagaag gcgagaatcc ctccagtaaa
     5341 tgcgacaatc gcaaatgcaa gggccggctg gtgaaaaaca aattcactta ataaagctat
     5401 cccaccaata agcaaagtaa gtaaaaattt tttgaaatgt gtcattgttt tttctcctct
     5461 tttatgtgac ttagagaggt tagtaataaa tttccagctg cctctttcat cttaagcata
     5521 gtccttgact agcacttttt aaaacaatag gccttaattg aaaacaacta tcactatcag
     5581 cgtttccaaa attcatcagt tctacaaaca aaaatattca gcaaggatta ctttttcgat
     5641 tcatcagtta aaaagtacct tttttcgaaa aactatgcta tacttttaat tcgttattta
     5701 cggcggaagg agcaaacatg cgatgaatga gaaacaacta gaacaaacgc acgtggatga
     5761 aacgatcgaa ctaatccaga tcgaacaaaa tatgctggat acaaaacaac aagaattgac
     5821 tcaggaaatg aatcattcga caaaagacac agctaatcac aagattcgag ctggttccaa
     5881 tgaagctttt tatgaatctg ctgttgaata tcgccaacat gagcaggaat tgcttttgaa
     5941 ataccatact cttgaagcac agcaaaaacg cctgcaaacg ttgtctgtca tgaaagatag
     6001 tccttatttt gcccgtatcg actttaaaga agaccgtgaa acagaaacat tatatctagg
     6061 tatcgcttct ttaagagatc tggcagaaga aacaatcgtt attgactggc gcgcaccaat
     6121 tgccaatctt tattatgaag gtgagattgg tcccgcattt tatgaaactg aaacagatag
     6181 aatagatgtc gatctgcagt tgaaacgaca atttaaaatc caagaaggac gtattttatc
     6241 tatggtagat acgtcggaag taatcaatga tgactttttg ctagagatat tggatgaatc
     6301 atcaagcaac catatgaaaa acatcgtctc tacgatacaa aaagcacaaa atgcaatcat
     6361 tcgggacaca acaagtaaag taatgctgat tgaaggaatc gccggaagtg gaaagacatc
     6421 cgctttgctt caacgtattg cttttctgtt gtatcataat cgcaagtggc tcgaggctga
     6481 aaacgttctt ctattttcac ccaaccattt gttttctgat tacatctcga ctgtattgcc
     6541 ttcattaggt gaaagcggcg tgcctacaca gacattcaaa aattatttgg tgcagctgct
     6601 gccagagttt tctttactag atgaacagca acaagaaagt ggttttttgc taggggaaaa
     6661 agatccgata caagtgatga aaagcggatt gaccttagtc gatcagattg atcgctatat
     6721 ccaaggcatc acaaactacg gtcccttgtt ccgtgatatg aaaataaatg gccgtacgat
     6781 cctatcaaag gaatcgattc gccaatggta taaagaaaca aatgaacaat tacctttaca
     6841 tcatagactt tctctattac aaacgaagct cctgaaaaaa ttaggcggtc tgcaaaaaga
     6901 cgaaacacgt cagcaatggg tcaaagatct ggcggaagaa cagctccaag aactatacgc
     6961 aacagatcca aatcttgaat atacagaaca aaaggagcgt tcattacgta aaaaactggc
     7021 caaccaaatc gtacgtaaaa gattccgtcg aatccatcga gggatcatga attatcaatt
     7081 tgtcaatata acgaaacaat atctccactt tctacaaact gtgccacttt ctcttctaac
     7141 acgacataaa atcagcgaga ccaggtggca ggaacatttg acaactgttc gtacacactt
     7201 gcgtcaaaga gaattgcgtc aagaggatgc tgtactattc tttttactga tgcgccgaac
     7261 atatccagtg acggttacac acaaagctcg ctatatcttt attgatgaaa tgcaggattt
     7321 cgcaccatca caagtggcat tgctgcaagc cttgttccca aaagcaaatt tgactttctg
     7381 tggagatcta aatcaaaatg tctttggaaa tgaaacgatc gttggatcat tggaagacct
     7441 tttcgtggac cgtgaagtca ctcgtttcca attaacgaca agctatcgct ccacaaaaga
     7501 aatcactgat tttgctaatc attttattgt gaaagaacat caagtagaaa caacagcaag
     7561 aaaaggagac cttcctttgc tcatccaagg aacaacagat gatatcaaat tgaattggtt
     7621 agcagatacc attcgaacga ctcagggaaa agcccggtat tggcgtacag caatcatagc
     7681 taaaacacaa gaagaatgcg aagctcttta tgctcaactg ccagacgagt tgaaacaaca
     7741 agtccagctg attttaaatg agagtgactt tatgaagcgt tccattattt tacttccagc
     7801 ctttttagca aaaggtctgg agtttgaccg agttttcttg tgggatgtag atgcagaaca
     7861 tttttcttct cagcaagaca aattgatcct ttacaccatg tgtacacgtg cgatgcatga
     7921 attgaccctt tttacctcca ataatgaaag tcctttgtta aacaatatgg atatttcatc
     7981 ttatgagaaa aaagtactgt aagtaaaaag acaactgcta tcattaatgt tagcagttgt
     8041 ctttttattt acaataataa ttgcagaaaa agtaattcaa tcatttaagg tcatttattc
     8101 tatatataaa tccaaatctg tttcaggata ttttaatttg acaagcgaaa taatcatttt
     8161 cagttcttcc atgttttctg gatattctgg gtcaaatacc ttgctatgac ggccaatccc
     8221 gccattgtcg atcaatcggt aaattcgatt ccattcaatg aattggtcaa ttgattggaa
     8281 attgctgatt ggatagccgc cacaatccaa aatctctgat acacgaaatg ctaggtatgc
     8341 actatcgatc actttatttt ttttcgtgtc tatcttctca tttctttcga taagaatttt
     8401 ttcaggacga tgtttcaagc tccactctaa tagtagaaag aaagcctcca tccgatactc
     8461 ttcttcaaat aaccgaaagg ctgaatcaga acgacgaaaa agcagcggaa tcagataatt
     8521 ttgtctataa atcagcattg gtgcgcggtc caccatacac tctaaataac ggttcagata
     8581 ccctgtaccg acatagtttc ctaatcgata gccttcaacg ccgaatcttg cgtatttttg
     8641 cgctaatttt gaacaatcag ttacgttcac agctaactct tctactttgc ggtaaccgct
     8701 taagatacgt tcaaaaggtt ctaaagtgat ttttgcttcc atattcttcc ccgtccatct
     8761 tccacatact tcatatcatt tcacatgata cttatgctct catcatacca caggttgaat
     8821 tagaaaagaa aataaatcac acaaaaaagc aagacaatct gttagtgaga ttgtcttgct
     8881 tttcgaaaaa gactgaatac ttttcgttaa aactggttgc ttagtcttct aatgccacat
     8941 tatggaacac atgctgtaca tcttctagat cttccagtgc atcaatcatt ttttcaaatg
     9001 tttcttgatc ttctggagat agtgttacct cgttttgagg gatcatttgg atctcagcga
     9061 ttgaaaaatc agtgattcct ttttctttta atgcttcttg tatactatgg aaatccgcta
     9121 cttcaccgta aacgatgatt tgtccttctt cttccattac atcgcgaaca tcgatgtctt
     9181 tttccattag gtattcaagg atctcgtcag cgtcttctcc tgcaaaagca aagattgctg
     9241 tattgtcaaa catgtaagaa accgctccgg aaacacccat gttcccgcca tttttcccaa
     9301 atgctgcccg tacgtctgct gctgttcgat tcacattgtt cgtcaatgta tcaacaatca
     9361 ccattgagcc gcttggtcca aatccttcat agcgcaattc agagtatgtt tcatctcctg
     9421 caccttttgc tttttcgatt gctcggtcaa taatatgttt tggtacatta taagttttag
     9481 cgcgatcaat gacgaaacgt agtttttggt ttgcatgcgg atcaggatca cctgattttg
     9541 ctgccgcgta gatttcaatc ccaaattttg cataaatccg gctattatta gcatctttag
     9601 cagttttctt tgctacgata tttgcccatt tacgtcccat attttcactt cgctttctga
     9661 tttgatagtt tcataacgca aaaacgtcct gttccattat acttgtgaat cacgattctg
     9721 tgaagctttt tttctttctt ccttcattct tctcattttt agtcaattat ctatactcgg
     9781 attgggattt gcccgaaatt ctgttccgga atattgcctc ccaattgata gatttgatca
     9841 cttctaacta acagttccgc ttcttgtgcc tctttttttc cgattcggga ataaatagaa
     9901 atctcttttt tttcttttaa gaatcttctt ccagcatcgg taaatccaag tagtcgaaga
     9961 taatttttat tttgttggac aggaatctct tgctttttga tattcagcaa gacataacaa
    10021 gataatcgtt ggatccgtgt ccatgtatat ctttttgttt ttacctttga cacaaaatcc
    10081 tcaaaactat cagcagttgc agctgctgtt ttcatgcgcg attctattcc ttcggtcatc
    10141 tgatagatat ctgtcagttc cagcaaagta gaagataaca accgataacg caaatacggc
    10201 caataagatt cccaagtctg cagcgaacaa gttgaaagat cttcttctgt ctgaggcggc
    10261 acaagtccag ctattctttt tccttcttct aacgcatggc gaatggctgt tgcactggct
    10321 atcctctgat ctgtgatgat tttttcatga tagcctgcac ttttacgtcg gatcggatgt
    10381 aattccatag ggtggacata cttggcattt tcctttgcat aacttaatcc caagatatga
    10441 tttggcgaag aaaaatcaag cgtaatatcg ggatacagac tgcgaaaaac ctcagtcatt
    10501 ttttgcggat aactatacgt ttgatcagac agttcttgaa atgcctcttc tatcctatct
    10561 tgattctctt ggacaaagcg tccgaaactg ctgtaatcaa acgaagtctc ttcttctgtt
    10621 ccaaaacaca aactgtcaca gtccagtgtt tgcaagattt ttactgcacc tttagcaaaa
    10681 tagtcagctg actgaaccgc ccattcgacg ggaagctcca ctactaaatc cacaccattt
    10741 tgtaaagctt cattcgcgcg atgccattta tcgatgatag ccggttcgcc tctttgcaaa
    10801 aaattcccgc tcattacagc aatgacgacc tctgctcctg ttatttttcg tgccatttcc
    10861 acatgatagc gatgtccatt gtgaaaagga ttgtactcta cgataatccc gcagcttttc
    10921 atgcgcatcc tccttcatag ttatcgataa aaaagaaata gagacaagta aaaaaaggca
    10981 aaaatcggaa aaaatacccg aactatataa actattctcg caaacacaat acttctgcga
    11041 cgaaaagctg cactgcaaga gcaatgccta tatcgttcgg atatataatt tatgttgaag
    11101 atttattctt ttcttgttaa ctcgagcagg caaaaccgct agtctttgta tgctacaaag
    11161 aaccagcgct ttgatttctc tgatgctgct tcgtctgtaa aatcagaaaa tgcttcgact
    11221 ttcgaaaagc cgctgttttc cagcattctt aaataacttt ccaatggata ggtacgctct
    11281 tgatgtagtt catcttcacg aataaatcga ccagaccctt cttgttcttc aacgaaaaag
    11341 gtaagaaaat gttcgatgct gtgtttctcg cttcctggat aactatccca caaaaaagca
    11401 aaattatccc cttgataatg atagctatat tctggaaaaa cctcatctac ttgataggtg
    11461 gagtgtacgt caaataaaaa tcggccattt tcttctaacg cttgatagac ttcatcaaac
    11521 acttgctgta cctcttgtct atttttcata tagcacagcg aatcagaaaa acaggtaatt
    11581 gcctgatatt gtccgatttc tgataaatcg agcatattgc cttccacaaa ttggatcgat
    11641 gcgtcttctt catatgctcg ctggctggca atcatcaaca tctcttctga aaggtctaac
    11701 cctgtcactt tatatccatc ttttgcaaag gcaaccgcta atgctccagt accgcatgcc
    11761 agttccaaaa tattttttgt gtcttcttgt aaatgacgtt tcgaaaaagc taaccattgc
    11821 tgataaagtg tttcgtccat cacttcatcg taaatgaaag caaatgtttc ataagccata
    11881 tgttaatcca accattcact aagatcgact agcggagcat ctgaccatag tttttccaga
    11941 ttatagaaat ttcgttctgg cgcatggaaa acatgtacga tcacatctcc aagatcgatc
    12001 aatacccatt ttccgccttc ttttccttca atacgtttta cttcatattt gttttcttct
    12061 tctttatcaa tgatttcttc tgtaattgcg ttgatttgac gttcgctatt tgctgaacag
    12121 atcatgaaat aatctgccaa caaggaaaca tttcgaacgt ctaaagcgat aatgtcttct
    12181 gcccgttttg aatctgctgc ttcaatcgca atttttaatt gttctttact atctatgatg
    12241 atttcctcct aatatgagca gcttattttt ttgctaccca gtaattatat gtttcgattg
    12301 tttttggata aatctgttgt tcttgcgcaa gcaaatgggc caatgtatgt tttgtttcaa
    12361 atgctacagc ttcgtctaga tcagcccatg caatggctcg tgcatcttgt accccaggaa
    12421 aatctcgacc tggttcaata tagtctgcta cataaatgat tttgtcaagg agactcatct
    12481 tagcagcccc agtcgtatgc acccggattg catgaagaat ctctgcatcg gtgattccta
    12541 attcccgctc tacaaacgaa gcgccaacaa gaccatgcca gattgcatta ttataattca
    12601 ataactccgg atcaaaaccg tctcttacga ttaccatttt gaactcctca tctggtcgtt
    12661 cttttgcata atcatgtgtc aatgcagcaa tactagcttt ttcaggagaa gcaccgtatt
    12721 ttttggcaag agccacggct gtttcttcta ctccaagcac atgcttgaag cgttgttcgc
    12781 tcattctcat ctgtattttt tgcatcaggt tttctctaga ataagcagta tattcattac
    12841 tatagtccat attcatacaa cccttcattt tgaatataat caattacttt atctggaaca
    12901 aggtagcgga tagaacagcc ttctttgatc ttttgacgta ttttcgtgga actgatgtcg
    12961 atctcaggta catctaccca aattacagga tatggagtat ccgttgtgta tcctggtcga
    13021 cgtataccca caaagttgac catagaagtc agctcatcga ttttatacca ttttggcaaa
    13081 tattctacca tatccccacc gataataaag taataatctg tatccggatt gttttgcgtc
    13141 aattccttca tagtatcata ggtgtaactt ttcccaccac gagctaattc aatcgtttca
    13201 atctgcaaaa aaggattgtc ttccaccgct aattccaaca tgttgagtcg atgttttgca
    13261 tcaatcgtct gtttctcatc aacatgaggt ggctgataag aaggcatcaa aaatacgcgg
    13321 tcaagaccta aattccggcc agcttgttca gccatcacta aatgtgcaag gtgaacggga
    13381 ttgaatgttc cgcccaagat ccctacttgc tttttcttct ctaagaattg aggcatttct
    13441 tcaggaaata attgtgaacg aacaaatgtc ttagcttgcg tattcatcat ggtcactcct
    13501 tatattgctt ttacttcttt tgatagcctt tgatactttt cttttgtgga aggtttgtat
    13561 aatacaataa cacgtccaat gatttggacg atttcgcaat ctatttcttt ttctaatact
    13621 tgagctgctt cttctgcaat ctcatctgta ttttgaagta acgatacttt gatcagttct
    13681 cttttttcta aagcttcttc aatttgaacg atcattgctg aattgatccc accttttccg
    13741 atttgaaaaa tcggctgaag atgatgcgct ttgctacgta aaaaacgctt ttgttttcct
    13801 cttaaactca taattttcct tcttccttaa atcaatgctt tgcgtttcaa tacatctaca
    13861 ccctttggcg cccagcccgc aattactact ggcgctgtta cagtgatcca gcctagaccg
    13921 gcaaaaacga tgtctgtttt ttctttcaca gaaaactcaa aacgaacaag ctccggaaat
    13981 gcctcgactt catcaggacg aggaggctgt aataatccac ctacatgttt ttgataaaat
    14041 tcgtctgctt tttccagttt cgttcgatga atatttacat catttgacac ataagcaata
    14101 aatgaagcac gattgccgct gataaaatca aatcttgcaa gtccgcccaa gaaaagtgtt
    14161 tgcccttcat tcagctgata aactttaggt ttgatttctt ttaacggtgc aatgatccgc
    14221 aaatcttttt tacctaagta gtgcgccatt tgatggcgat ggataattcc cggtgtatca
    14281 atcaaaaaat gcccgtcatc taaaggaatc tcgattttat ccaaggtcgt tcctggaaac
    14341 tgcgaagttg tgattacatc ttgtactccc gctgtatttt tgatgatttg attgataagc
    14401 gtcgattttc cgacatttgt tactccgacg atatagacat cccgtccatc gcggtattct
    14461 tcgatttttt ctaatagttc cgtcatctca tgagatttct tagcactagt taataataca
    14521 tctactggac gaattcctgc ttcgtaagca cgttccttca tccattgtgt taatttagtt
    14581 tttttcaatg atttaggtaa tatatccact ttgttcccaa ctaaaagtac tggattgtca
    14641 ccgacaaaac ggtgtagtcc tgggattagt gaaccattga agtcaaaaat atccaccaca
    14701 ttgacgatca atgcatcttt ttgccctaat tcattcagta agcgcaaaaa gtcgtcatcc
    14761 gtcaatgaga catcttgggt ctcattataa tggcgtaatc tgaaacaacg ctgacaatag
    14821 acttccccgg aagctaaacc tttttccaaa gccgtttgcg gcgtgtatcc taattcaccg
    14881 ggtttgtttg tttgtataac ggcaccacag ccgatacatt gtaattcttc tgtcattcga
    14941 ttccacctcg ccagcccata tcagggtgtt ttgctaatag ataggccata attctttttt
    15001 cccaaaaacg attaaattgt gtcttccaac tatctgttgc gataatgggt tttacaagga
    15061 tgctacgtat ccctgcacga ttggctccac gtatatcagt cataatctga tcgcctacca
    15121 ttactatctc atcatctgct aaatccaatt gtttctttgc tatgttgatc cctcttgcta
    15181 aaggtttcat tgctcgtgca acatacaaaa gatcaaattt ttcaatcgct cgcgcgacac
    15241 gttttgaatt attgtttgaa acaacaacaa cagggattcc tgcatttttc atttctaaaa
    15301 tccaagtaag caactcttct gttccatctg gattattcca tgcaatcaac gtattgtcta
    15361 aatcggtcaa tactgctttg atgcctagat tctttaattg ggcaggtgta atctgataga
    15421 tggcttctac catccatgtt ggtttgtaat ttgaaaacat gctgtctcct catctgcaaa
    15481 aaatgaagga gcacataaag tactcctcaa ccccgtatta tactgcatat cagcaaaaaa
    15541 aactacctct atactcgttc gtctttcaga aaaagcattt ctttacaaaa tgaccaaaaa
    15601 taaagattga tcacagaaaa atttagaaac aaaaaacaac taatattggc ggaacctctt
    15661 gtatcgtttt tccagtaatt tatgctgatc taatcgaaca agttccgtca atctttcaat
    15721 caacgctttt ttcataatgt gtgcgacttt atatcgtggc aactgctcgc cgttgaacgt
    15781 ttcaggaata actcgatcaa cgattcctaa ctcgcgtagt tctggcgcag taattttcat
    15841 caactcagct gcttctgcgg cgcgactacc gtctttccac aaaatcgacg caaatccttc
    15901 tggagaaagc acagcataaa tcgtttcttc cagcatccag acttcatccg ctaaacctag
    15961 agcaagggca ccgccacttc caccttcacc aatgatgatg gcgatgatcg gtacctttaa
    16021 atcagacatt tcgagcagat ttctggcaat agcctctcct tcgccgcgct cttctgctcc
    16081 tacaccgcaa tatgcacctg ctgtattgat caaggtaatc accggtctat tgaacttttc
    16141 cgcttgtttc atcagtcgta aagcttttcg atagccttca ggatggggag aaccaaaatt
    16201 tctgcggata ttttcctgca gatcccgtcc tttttgaata ccgatcactg ttaccggttt
    16261 gccatctaat cgagcgattc caccaacaat cgcttcgtca tctgcaaata aacgatcacc
    16321 atggcattcc tcgaaagaat cgaagatctc ttcaaaaaaa tccaagcttg tccatcgttc
    16381 tttagctctt gctaactgaa cgacatcatg tgctgtctta tccacgatct atccatcctt
    16441 tctgtgtatg caattcaaga agcgtatgga ttttctgttt caattccatg cggggaacaa
    16501 tctgatcgac aaaaccatga gataataaga attcagcctt ttggaaatct tctggaagtt
    16561 cctgcttgat cgtctgttcg atgacgcgtc ttccagcaaa accgatcaag ctttggggtt
    16621 ctgctagaat aatatcgcct tccattgcaa aactggcagt tactcctccg gtcgtcgggt
    16681 ccgttaaaac agttagataa aaaagacctt cattgctatg ccgtttgaca gcagcagaga
    16741 tttttgccat ctgcattaga gaaaagattc cttcttgcat ccgtgcgcca cctgaagctg
    16801 tgaacaagac gactggaaga ctttcctttg ttgctcgttc gaataatctg gtgatctttt
    16861 ctccgacaat cgttcccata cttcccatga taaaattagg atccatcact ccaataatga
    16921 agggaatccc ctgaatggtt gctttaccag tgaggactgc ttcatgaagt cctgttttat
    16981 cttgcatttt tttaattttt tccgtatatt cgggaaatgc tagcggatct ttggttacaa
    17041 gatccgtatc ccattcttca aaactttttt catcaataat caaagctaga cgctgccatg
    17101 caccgattcg aaaagcgtat ccgcaatgag ggcatacttt ttcttcaccg atgtctttcg
    17161 tataaaggat atgtttgcaa tttggacact ttgcccacat gttatcagga acagatggcg
    17221 agttagcagg tttgctctct ctattaggat tgatccgaat atatttcttt ttcttaaaca
    17281 gagccatcta tgcgcctcct atctccgttt ctggtgtcca attcggtaaa aattcttctt
    17341 gtaaaaatgc tgtgctgtaa tcacctgcta tgactgcagg atgactaatc aaatccatct
    17401 gaaattctgc gttcgtgatg attccttctg tgataaattc acttaaggct cgctgcatct
    17461 tcattaatgc ttcaaaacgc gtatttccat ggacgattat ttttgcaatc attgaatcat
    17521 aatatggcgg gatcgtatat cccgggtaaa ctgcactgtc tactcgcagt cccatcccac
    17581 ctgctggaag cagtaagttt tggatcttcc ctggagaagg agcaaaatgg aaagcaggat
    17641 tttctgcatt aatccggcat tcgattgcgt gtccttggaa tactacttct tcttgacaca
    17701 cattcaaagg ctcaccggct gctatctcca cctgtttttt tactaaatcg attcctgtga
    17761 ccatttctgt tactggatgt tccacttgga ttcgagtatt catttccatg aaataaaaat
    17821 cgccttcttc atccatcaga aactcgatcg ttccggcatt ttcataattg actgctttag
    17881 ctgctcgaac agcagcgtct cctagtgctt ctctttttgt ttgtgagatc actacagaag
    17941 gagattcttc cagtactttt tgattgttgc gttgtaaaga acaatctcgc tcgcctaaat
    18001 gaatgacatg accaaatcga tcacctaaga tctgaacttc tatatggcga gcaggataaa
    18061 tgattttttc caaatacata tcatcatttc caaatgccgc tgctgcttct tgctgagctg
    18121 aagaaaaatg ctgtgggagc tcttcttttg attgaacttt ccgaatgcct ttcccgccac
    18181 cacctgcagc tgctttcagc atcactggat aaccaattct gtctgctatt tccaatgctt
    18241 cttctactgt agataataca ccagtacttc ctggaatgac tgggacattt gcttcttgca
    18301 tcaattgacg cgcattgatc ttatttccca ttgcatcgat cgttgcactt ttaggaccaa
    18361 taaatgtgat attgcactct tcacacatgg aggcaaatcg gctattttca gataaaaaac
    18421 caaatcctgg atgaattgct tcggcttttg tcacgattgc agcgctcagt acttcctgaa
    18481 cattcagata tgaatcaacg gcttttgccg ggccaatgca gattgcttca tctgctaact
    18541 gtgtatgaag tgcttcggca tctgcttctg aatatatcgc tacggtttgg atccctagtt
    18601 cgcggcatgc acgaatgata cgtacagcga tttcaccgcg gttagctatc aatactttag
    18661 aaaacatatt tcacctatcc aatcataaag gttaattccg cttcggctac ttttttaccg
    18721 tctactttag cgattccttt gcctattccg gctgaagctt tcactttcaa tatctcaact
    18781 tcaaggtata aggtatctcc cggtgtaact ttcttacgga attttgcttt gtccaatccg
    18841 ccgaaataag ccgtctttcc cttgaattgt tcaagagaaa gtaatgcaac tgctcctgct
    18901 tgagccattg cttcaacaat caatacccct ggcataacag gttcttctgg aaaatgtcct
    18961 tgaaaaaagg gctcattgat cgttacattt ttcttagcaa cgatccgttc tccttcaacc
    19021 atttcttcca ctcgatcaat caaaagcatt ggataacgat gtggaatgat ttcttttatt
    19081 tcttgtatgt tcattgtttt ccctctttcc tgctttgctt atttttgctg aatcctaaag
    19141 agtggctgtc catattcgac aactgactcg ttttctacaa ggatttctgt gatcactccg
    19201 tcggcctcac tagttatctc attcatcagt ttcatcgctt caataatgca gataacttct
    19261 cctttggcaa cttgatcacc tacttttttg aaagctggtt tgtcgggcgc tggttgcaga
    19321 taaacgattc ccacgattgg cgaaacaacc tcgattccat caaatactgc cgattctttt
    19381 tctttcatta tttctggagc agttgttgct tgttcaggta tgacattgac tggctccgaa
    19441 actggcaacg tttcgtttgt ttgaaccata ttcatgcctc gacttgtttt atttttattc
    19501 atatacaatt caaattgacc ttcacgcaaa tcgaattccg ttagcgagga ttgatcaaat
    19561 tgactgacta attctctgat ttcattgata tccaaaatta ttcctcccag cgtttcatgc
    19621 acagaaccgc attatgtccg ccaaacccta atgaattact cattgcataa gtaaaacttg
    19681 caggtttgct ttcgtttttg acaactgtca agtcaatctc aggatcttgt ttttctacat
    19741 tgatcgtagg tgggataaat tgatgttcta atgccagcaa agttgccaag gcttcgatcc
    19801 cgccggctgc tcccaataaa tgtccagtca tcgatttcgt gctgctgacg taagtatctc
    19861 tgaaattttc acctaatgcg atttggattg cttttgcttc tgcctggtca ttggctggtg
    19921 tacttgttcc gtgagcattg atatatccga cttgttccgg ctgtacacca gcttcttcca
    19981 tcgcaagctt cattgcttta gatgcaccag aaccatctgg agtaggagct gtcatatggt
    20041 aagcgtcaca gtttgcaccg tatccaacga tttctccata gattttggca ccgcgagctt
    20101 gtgcatgttc taaagattca agaactagaa cgcctgctcc ttcacccatc acaaaaccat
    20161 tccgattttc gtcaaacgga atcgaaccac gattaggatc agttgattct gagagtgctg
    20221 ttaaagctgc aaatccagca atccccatct cacaaatcgt tgcttctgtt cctccagcaa
    20281 gaatgacatc agaatagcca tgtttgatgt tacggaatgc ttccccaatt gaattcgttc
    20341 cgctagcaca agctgttacg atagctgtac aaataccttt tgcacctaca cgcaaagcaa
    20401 tatttccagc tgccatgttg gcaatcgcca ttggtacgaa cattggagaa actcgttttg
    20461 cacctttttc atgtagacga ataacttggt tttcgattgt tggaagacca ccgatcccag
    20521 atccgatgat gacgccaaac cggtcttgat tcattttttc tttatctaaa ccgctcatct
    20581 ccattgcttc taaagcagcg tagattccat agatcgaaaa catatccatt cgtttgctat
    20641 cttttttaat aaaatattta tcgtaaggga aatctttgac ttcacccgct aaggtaatcc
    20701 ctgtttctga agcatcaaat ttagtgatcg gtgcgatgcc attcgtcccc tcttttaagc
    20761 tattcaaaaa gctgtctgcg tcatttccaa taggtgaagt gactccatag ccagtaatta
    20821 ccactcgatt catttgtttg ctctcctttt cttatccgtg catgaccaat ccgccatcga
    20881 cattgatgac ttgtccggtg atatatggat tttgtgctaa gaaaactgct gtttgtgcaa
    20941 tatcttctac ttgtccgaaa cgctgcatcg gaatttgctt ttccgcttgt tccttgactt
    21001 tgtctgctaa aacttccgtc atatcagttg tgatgaatcc tggcgcaatg gcattacatg
    21061 taatgcctct agttgcagct tctcgtgcca cagattttgt taatcctact acgccagctt
    21121 tactagctgc ataatttgct tgaccaatat ttccaatcaa cccagataca ctagagagat
    21181 taatgatggc cccttctcgc tgcttcatca tttttttcaa tacgtgctgc gtcatattga
    21241 aggttccaat caaattgata tccagacatt ttttgaaatc ttctgcatcc attcgcatca
    21301 caagtttgtc atttgtgatt ccggcattgt ttactaacac atggatcgat ccaagctttt
    21361 cttctgcttc tttgatcatt tgtcccgctt tctcataatc agagatgtca ccggaaactc
    21421 ctacgcattt tacaccgaat gcttcgattt cttctatctt ttcttttgga atctctccgc
    21481 gaccattcaa aatgatattt gctccagctt ttgcaaaagc taaggccatc gctttgccga
    21541 ttcctcttgt actacctgtg ataaatacat tttttccttt tacatccata acgttcctcc
    21601 ttatagtatt gctattgttt catcaaatgt ctgcttattc tcaacacgca gaactggtat
    21661 tgttttatcg atttttttca taaatccgct taatactttt ccggggccga cttcgatgac
    21721 ttgttcgatg ccgattgttt ttaacttatg gatactctca tagaaacgaa caggttgcat
    21781 gacttgctgt tcaagtagtc ccgcgattgt ttctttcttc attatttccg ttgttgtgtt
    21841 gcttatgata ggaaaagaag gttcagagaa ttgaatctga cttaggtcct tcgccagttt
    21901 ttttgctgcc ggttctaaaa tagctgtatg aaaaggcccg ctgacattta gagggatcat
    21961 tcttttgaac cctttttctt ttaatagtaa aacagcttcg tctactgctt tttcttcgcc
    22021 accaatcacg atctgctgag gtgtattata atttgcagga gatacgatgc cgtacttgct
    22081 agcttcatga cagctttcct cgatcgtttc aatcggcgca ttcatcactg caaccatttt
    22141 cccgcttcca gctggggcag cttctgtcat ataagcgcct ctttttgcta ctaacgcaac
    22201 tgcttcagtg aaggatagcg cattactagc tactaatgcc gaatattctc ctaaactcag
    22261 tcctaaagct gcatctggga tgattccatg ctccttcaat aagcgataat acgcgatgct
    22321 gactgttaga atagctggct gtgtatattg cgtctgatcc aaacgttcat tttctgtaaa
    22381 gcaaagttca gccatatcgt agccgagaat ctcacttgct tcatcaaatg tctgctttac
    22441 aacggcttct tcatataaat cttttcccat accttgatat tgtgcacctt ggccactaaa
    22501 taagaatgct gttttcatgt ttttctccaa tatatccaga atatatgttt gattcttcct
    22561 attcaaaaag gccgagacat tcctatttca ggaaatgtcc cagcccccaa cctaatttta
    22621 atgattatgc agtttgtttt tctacgtaag tcactaaatc ttgaaccgtt ttgattccgt
    22681 cttctgtttc gattttcacg tcaaattcat cttcaatttc attgatgatt tggaataaat
    22741 ccaagctgtc agcttctaag tcttcttgga tgtcagtagt caatttgatt tcttcagttt
    22801 cttttcctaa ttcttctgcg atgatttctt gaattttttc gaataccata ataatatatt
    22861 cctccgatat tgtttgcttt ttatttttat aatttaacag tctacaactc catgagaata
    22921 cttccccatg ttaaacctcc gccatagcct gtaaatatta ctttctgctg ttttcctaag
    22981 gtgatctttc cactagaaac tgcttcatcc aataagatag ggatgcttgc agcagatgta
    23041 ttcccatatt tatccatatt ggtcaggaac ttttcttgcg gcatctttac ttttcgggca
    23101 attttctcaa taattctgag gtttgcctga tgcaaaagca gataatcaac tggtttgtct
    23161 ttcatgactt cttggatatt gtttgcaacg tctcgtacgg caaaatcaaa aatatctctg
    23221 ccagtcattt gcaaataata cgaaccttct gaatcttctg aatagaacgg atttttattt
    23281 tgctggtagc cagcggtcaa tgagctactt ctggtaccgt cagctgctaa tttttctttc
    23341 agcaattttt tctgtggatt cgcttctaag agaatcccac ctgctccatc gccaaacaac
    23401 acagcagtcg tacgatcaga ccaatcgatt agcttagata gtgtttcacc accaatcacc
    23461 agtcccttag ttgagccgca gcggatcatt ttttcagcca tgcttaacgc ataaacaaat
    23521 cctgagcacg cagcactgat atcaaaagcg atagcatgag ttgctcccaa tttcccttgt
    23581 acttgtgctg ctacagatgg tgtcgtgtaa tcgggcgaca ttgttgctac taaaataaaa
    23641 tccaaatctt tagggtttag atttttctgt tttatcaatt tttccgccac taaaatgcat
    23701 aaatcagaag tctcctgatt tgttacacat cttcgttcac gaatacccgt ccttgacgaa
    23761 atccattcat cagaggtatc catcatcttg gaaagatcat tattagtaat aatcttttca
    23821 ggaaggacgc tggctgttgc agtaatcatt ccaaattgtt ccattaaata tcctcactta
    23881 tagtcttgca agaagtcgtg caagtttttc agtgcatcca gcaaggcatg ctgttcctcc
    23941 gtagccattc cgtctaagat gttcttgacc atttcacgat gaaaatgctg atggacccga
    24001 aatagtaact ttcctttttt cgttagtccc aactttacaa cgcggcggtc atcttcactg
    24061 cggatacgtt cgacatagcc tttttttact aacttgttaa tagcagtggt caaggtccca
    24121 acagtaattg atagttcttt ggctacttct gaagttgttt ttttcttgta catcccgatt
    24181 gcctcgatcg tgtgcatctc tgtgattgac agatctttga attgagattt tttcagctca
    24241 gactcttcga ttgtcaaaat atcattgaag acactaacaa gataatcatt tactgttttt
    24301 aaatctggtt ccatgttcca ccgtccacta attagtttga ttatcaaatc ttttgatgtt
    24361 caaactatat cttaactttt ttcaaaatgc aaccttaaaa gatcattttt ttactatatg
    24421 agcttttttt aagattttac tcatttaaaa caactgataa atcagcattt ataatttctt
    24481 ttattagaaa aatcatctat ctcacatatt ttgattatca aagtattttc tgtgaacaga
    24541 actatagaat tccctaaaac ttgtccatta tttcaaaatt tgttatagtc tttttattca
    24601 aagcgtaaaa ggagatccta tgttaaaaga aaaaatgaaa actctcccca tgctgccggg
    24661 tgtctatctt atgaaagacc gagatggtaa ggtcatctat gttggtaaag caaaacggct
    24721 caaaaataga gtaagcagct attttcatca gaataaacag cattcaaaaa aagtattacg
    24781 tatgattcat catataacag attttgattt cgtcgtagta gacacggaat tagatgcttt
    24841 attgctcgag tgtcaattga tccaacatta ccgtcctttt tataaccgac aaatgaatta
    24901 tttcagtaat tataattatg tacatattac caataaggga tttgtattaa cagatactcc
    24961 tacggctcga acatacgggc cttttcgtct ctataaaaaa atgccgtcta ttttgcgtat
    25021 aatggaagaa acttatcaaa tgccttggtt gtctgaaatt agtttgttag ctttgcgagt
    25081 ccagcttcct gacctacagg aaatgacttt tgaacagaaa aaaaaggaac tccaagggct
    25141 gtttcaaggc cgaaataaaa agctactgac ttacttgaaa aaaagacaac aacatttcat
    25201 ctatcagttg aatttcgaaa aagccggtat gcttcaaaaa gatatcgaat tagttactta
    25261 ttttattaga cggatccagg aacaaaagca gtttttaaga acaccatcgc tcaccttcag
    25321 catgccatta gcagcagatg agagtcagaa aaagcactac ctcgtttgtt atggtcaact
    25381 tgcagaaaca atgattacct ctggtgacgt ttctcctgac ttctattatg aaaagaaaga
    25441 agctcattta tctttgaaac gtcaattgtc aaaagaagaa atcgatcctg tacaaatctt
    25501 gatcagttac cgcaaaaaat tagaaaaaga acagtttgaa attgaactgt taaacaaaaa
    25561 agaagctgaa aagcaactaa attagttgct tctcggcttc ttttaggcat cttcaacttc
    25621 aaatgtcaat tatcctaaaa tttattggat taagtcatag atttccatcg ctacgatatc
    25681 gatattatca aattcgtatt gctgatgagt atctgtttca gttaattcaa atgtatcggt
    25741 cgctttatca taagttactg tacacttctc tacgccttct ttttcaaaac ggcgaacttg
    25801 cagttcatta tctgctgact cgaccatagc ctccagtcgt tttatgattg cgactaattg
    25861 tgaatgtgtc atcggaggtc ctccttttct tatttctgtt atattgtatc aaaagcatag
    25921 cctacttgca ttgattttct ctaaaaaaac tcgtttttta tgattttttt tctggtattt
    25981 ttccttcagg ccaccaaagt ttgatcaaag cttgggttcc gtcattctca aaacctttat
    26041 ctgccaactc ttcatatagt ttcaatgctt gggtagttgc tggcaataca agatccattt
    26101 tttctgcttc ttctaatgca atctttaaat ctttgataaa atgtttgaca aaaaaaccag
    26161 gtgtgtaatc ttcttttaaa atacgaggag aataattgct taaagaccag ttagctgcac
    26221 ttccacctga aagcgtctca atcactttct ttaaatcaag tccgtttttt tgtgcataga
    26281 caagcatttc cgtcattcct gtcatcgtac ctgcaatcat gatctgatta gccatttttg
    26341 tatgctgacc tttcccagca gaaccatgca atgtaaatgt cgtaccaaat tctttgaata
    26401 aaggcagtac ttgatcataa acggcttctt ctccaccgac catgatcgtc agtgtaccat
    26461 tctttgctcc taagtcacca ccggataccg gtgcatctaa cgcatctgcg cctttttctt
    26521 ttgctgtttt tgcaattttt tctgccaggc ttggtgtact tgtagtcaaa tcaactaaaa
    26581 tatgtcctga gatatctgca gaaaaaatcc ccgactgtcc atagtagatt tcttctacat
    26641 ctttggggta acctaccatt gtaaagagaa tattactttg ttctgctacc gcttttggtg
    26701 tatctgccca gatagctcct tctgttacta aaggatcagc ttttgctttt gtccgattat
    26761 agacagctac ttcgtgtcct gcttctagta gatggcgtgc gacagcactt cccatgacgc
    26821 ctgttccgat aaaaccaagt ttcatttttt cgacctccta aaaatcaaaa atccttgctt
    26881 ctatgctagt atacgctaga aacaaggatt ttttcataaa aaatgtttga attttatctt
    26941 ttaaatgaat ttaattttat tttgattcgt tcaatcgagt cttcttcttc ttttcttcgc
    27001 atctcttctt catgataaac aaccatgtct tgataaaatt cgatcatagc ttcgccgaaa
    27061 tgtgtagatg aaatatcata aagtttagct tctaaaactt ttggatcttt aggaacttca
    27121 ctggaaatat aatcaataag tgctggtgca aagtcttcgt cggtttcaaa agttttccct
    27181 aatgaaggat gatcgaacaa tcggttcaaa taagcatttc cttccacgac acaaggtgtt
    27241 ccggctgcca tcgcttctgt ataagtaagc ccttgtgttt ctgaagtaga agcactgaca
    27301 aaataatctg ccgctttata ataaagtgct acatcattgt tgtccacttc tcctgtgaac
    27361 tgcacaaatt cttctaactg cagttctgag atcaattcct ctaactcttc tacataaggt
    27421 ccttttccta cgataactaa tctggcgtta ggataagcgg agatgacctg aggaaaccca
    27481 ttaatcaagg cttggatatt tttctcataa gaaatcctgc ttaaagaaag aatcatcaaa
    27541 tggttttcct gaagacctag tgactccctt aatttgctga tatcctgctg agtgatatca
    27601 gaacgtttga atttttcaat gtcgatacca gtaggaatca cgcgaagcgg tgctattaca
    27661 ccatagctgc gtaatgtatc aataacacgt tcacttggac aaactacacc tgtcgtatga
    27721 tttgcaaata atctagacaa gtattttaca tgggattgcc tgatcacttt tcctttaccg
    27781 atataatgaa ggtagtcttc atacatcgta tggtaagtat ggatcagagg tattttcaac
    27841 ttcttagcta ccatcttccc aagtatccct gctccgaatt ctgtatgggt atgaataaga
    27901 tctagttcta gttctttggc aaccaaataa gcataccaca tgcctctaac aacaactctt
    27961 cgatccttga aagaaataaa tggtacactt ggcattcgaa tcacatcttt ttccaactta
    28021 tccgcattag gatcggtcgt tgtaaaaata tagacttcat gcccatgtct ttctaattct
    28081 tgttttaatg ttttgatcga tgtagcaact ccgctgactt gcggaaagta ggtatcggta
    28141 aaaaagccga ttttcatgtt tttcgcctcc aaactagtcc ttgacctgat ttactttttt
    28201 taatttgcta gagacttttt cattcagtac actttcgtat acatttttaa gttcttcacc
    28261 gattttattg atacttcgct cttttgctgt ttgataaccg gcttgtgtcg tactcggtag
    28321 ttcttgattg gtaatctgtt ggatcagtcg gataaattct tcgtttgtct tccccatata
    28381 gcagttcttg ctatcttcta accagccatt atacactgga atatctcgaa caagtacgtt
    28441 ttgtttgctt gctaaagctt ctaaaacaac gatcccttct gtctcttcat aagaagggaa
    28501 aaagaataga tctgcacctg aatacgcacc ttctatgatc tctccacgaa tgtaaccagg
    28561 aaacaggaca ttttcaggat gatcttcttt tacaattttc cggatgttcc gaggaatcga
    28621 gtacatggga acatgaccaa accaaataaa tgtgtattcg ggcatcttct tagcaatttc
    28681 tacgaaatca atgattcctt tacgttcgaa aaacaaaccg acacaaatga ttactttttg
    28741 atccggagac agtttgaaat attctctgaa cttttgttct ttcattggat cagggtgata
    28801 tttctctaag tcgatcccat tggaaatagc ttgaatcggc acacggatac catagctttc
    28861 caacaactgt tttgaataag gtgttggcgt gatcagatag tccgcttttt ggtacaatcc
    28921 taccagatat ttttttacga taggagaaag ctggttcgat ccgataaagg aatttcggaa
    28981 atcttcctct gtagaatgcg catgatagac aacttttttt ccttctcttc tggctttatt
    29041 gaccatatta tgactattga ttccgtatgt attgatgtgc aggatgtcat aatcttcttt
    29101 ttcatctagt gtataagaga taccgacttc cgtcagagca cgtttttgat gatcaagcgc
    29161 acgcccgatt ccagattttg caagcatctt ttcactttca aaatataata ataccttcac
    29221 cttacgtccc ccttcactta tatagtataa catagcctac aaactgtcac tcaaacttaa
    29281 gccctatttt t
//
LOCUS       DARBET010000028        28861 bp    DNA     linear   BCT 07-NOV-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4027
            SAMEA114569841-rid20112183.denovo.028, whole genome shotgun
            sequence.
ACCESSION   DARBET010000028 DARBET010000000
VERSION     DARBET010000028.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA114569841
            Sequence Read Archive: ERR12203666
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 28861)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 28861)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001972618.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001972618.1
            Genome Coverage       :: 132x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/07/2023 13:18:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,041
            CDSs (total)                      :: 2,975
            Genes (coding)                    :: 2,908
            CDSs (with protein)               :: 2,908
            Genes (RNA)                       :: 66
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 67
            CDSs (without protein)            :: 67
            Pseudo Genes (ambiguous residues) :: 0 of 67
            Pseudo Genes (frameshifted)       :: 26 of 67
            Pseudo Genes (incomplete)         :: 28 of 67
            Pseudo Genes (internal stop)      :: 20 of 67
            Pseudo Genes (multiple problems)  :: 6 of 67
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..28861
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA114569841-rid20112183.denovo.028"
                     /isolate="VRE4027"
                     /db_xref="taxon:1352"
                     /country="Denmark"
     gene            12..84
                     /locus_tag="SCJ86_001391"
     tRNA            12..84
                     /locus_tag="SCJ86_001391"
                     /product="tRNA-Val"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:45..47,aa:Val,seq:tac)
     gene            88..160
                     /locus_tag="SCJ86_001392"
     tRNA            88..160
                     /locus_tag="SCJ86_001392"
                     /product="tRNA-Lys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:121..123,aa:Lys,seq:ttt)
     gene            168..249
                     /locus_tag="SCJ86_001393"
     tRNA            168..249
                     /locus_tag="SCJ86_001393"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:202..204,aa:Leu,seq:tag)
     gene            267..339
                     /locus_tag="SCJ86_001394"
     tRNA            267..339
                     /locus_tag="SCJ86_001394"
                     /product="tRNA-Thr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:300..302,aa:Thr,seq:tgt)
     gene            351..422
                     /locus_tag="SCJ86_001395"
     tRNA            351..422
                     /locus_tag="SCJ86_001395"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:383..385,aa:Gly,seq:gcc)
     gene            439..524
                     /locus_tag="SCJ86_001396"
     tRNA            439..524
                     /locus_tag="SCJ86_001396"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:473..475,aa:Leu,seq:taa)
     gene            535..608
                     /locus_tag="SCJ86_001397"
     tRNA            535..608
                     /locus_tag="SCJ86_001397"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:569..571,aa:Arg,seq:acg)
     gene            618..691
                     /locus_tag="SCJ86_001398"
     tRNA            618..691
                     /locus_tag="SCJ86_001398"
                     /product="tRNA-Pro"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:652..654,aa:Pro,seq:tgg)
     gene            711..783
                     /locus_tag="SCJ86_001399"
     tRNA            711..783
                     /locus_tag="SCJ86_001399"
                     /product="tRNA-Ala"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:744..746,aa:Ala,seq:tgc)
     gene            811..884
                     /locus_tag="SCJ86_001400"
     tRNA            811..884
                     /locus_tag="SCJ86_001400"
                     /product="tRNA-Met"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:845..847,aa:Met,seq:cat)
     gene            898..971
                     /locus_tag="SCJ86_001401"
     tRNA            898..971
                     /locus_tag="SCJ86_001401"
                     /product="tRNA-Ile"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:932..934,aa:Ile,seq:cat)
     gene            995..1084
                     /locus_tag="SCJ86_001402"
     tRNA            995..1084
                     /locus_tag="SCJ86_001402"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1031..1033,aa:Ser,seq:tga)
     gene            1097..1170
                     /locus_tag="SCJ86_001403"
     tRNA            1097..1170
                     /locus_tag="SCJ86_001403"
                     /product="tRNA-Met"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1131..1133,aa:Met,seq:cat)
     gene            1189..1264
                     /locus_tag="SCJ86_001404"
     tRNA            1189..1264
                     /locus_tag="SCJ86_001404"
                     /product="tRNA-Asp"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1222..1224,aa:Asp,seq:gtc)
     gene            1266..1336
                     /locus_tag="SCJ86_001405"
     tRNA            1266..1336
                     /locus_tag="SCJ86_001405"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1298..1300,aa:Gly,seq:tcc)
     gene            1350..1423
                     /locus_tag="SCJ86_001406"
     tRNA            1350..1423
                     /locus_tag="SCJ86_001406"
                     /product="tRNA-Ile"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1384..1386,aa:Ile,seq:gat)
     gene            1448..1535
                     /locus_tag="SCJ86_001407"
     tRNA            1448..1535
                     /locus_tag="SCJ86_001407"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1482..1484,aa:Ser,seq:gct)
     gene            1557..1628
                     /locus_tag="SCJ86_001408"
     tRNA            1557..1628
                     /locus_tag="SCJ86_001408"
                     /product="tRNA-Glu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1590..1592,aa:Glu,seq:ttc)
     gene            1651..1740
                     /locus_tag="SCJ86_001409"
     tRNA            1651..1740
                     /locus_tag="SCJ86_001409"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1687..1689,aa:Ser,seq:tga)
     gene            1753..1826
                     /locus_tag="SCJ86_001410"
     tRNA            1753..1826
                     /locus_tag="SCJ86_001410"
                     /product="tRNA-Met"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1787..1789,aa:Met,seq:cat)
     gene            1845..1917
                     /locus_tag="SCJ86_001411"
     tRNA            1845..1917
                     /locus_tag="SCJ86_001411"
                     /product="tRNA-Asp"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1878..1880,aa:Asp,seq:gtc)
     gene            1921..1993
                     /locus_tag="SCJ86_001412"
     tRNA            1921..1993
                     /locus_tag="SCJ86_001412"
                     /product="tRNA-Phe"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1954..1956,aa:Phe,seq:gaa)
     gene            1998..2068
                     /locus_tag="SCJ86_001413"
     tRNA            1998..2068
                     /locus_tag="SCJ86_001413"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:2030..2032,aa:Gly,seq:tcc)
     gene            2081..2154
                     /locus_tag="SCJ86_001414"
     tRNA            2081..2154
                     /locus_tag="SCJ86_001414"
                     /product="tRNA-Ile"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:2115..2117,aa:Ile,seq:gat)
     gene            2166..2237
                     /locus_tag="SCJ86_001415"
     tRNA            2166..2237
                     /locus_tag="SCJ86_001415"
                     /product="tRNA-Glu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:2199..2201,aa:Glu,seq:ttc)
     regulatory      2426..2505
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF01767"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="SAM riboswitches modulate the expression of
                     downstream genes in response to levels of
                     S-adenosylmethionine. SMK box riboswitch (aka SAM-III)
                     shares no known similarity to SAM-I and SAM-II; Derived by
                     automated computational analysis using gene prediction
                     method: cmsearch."
                     /bound_moiety="S-adenosylmethionine"
                     /db_xref="RFAM:RF01767"
     gene            2503..3696
                     /locus_tag="SCJ86_001416"
     CDS             2503..3696
                     /locus_tag="SCJ86_001416"
                     /EC_number="2.5.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318393.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methionine adenosyltransferase"
                     /protein_id="HEG1168962.1"
                     /translation="MVERHLFTSESVSEGHPDKIADQISDAILDAILKQDPTARVACE
                     TSVTTGLVLVFGEISTTAYVDIQKVVRETIKEIGYTRAKFGFDGDTAAVLVAIDEQSP
                     DIAQGVDEALEIRDEDKKDVLDEIGAGDQGLMFGFAVDETPELMPLPIALSHRLVRRL
                     ADLRKSNELTYLRPDAKSQVTVEYDDQGQPERVDTIVISTQHDDAVDNETIRHDVIEK
                     VVKEVIPAELLDDQTKYYINPTGRFVIGGPQGDAGLTGRKIIVDTYGGYARHGGGAFS
                     GKDATKVDRSASYAARYIAKNIVAAGLARKAEVQLAYAIGVAQPVSISINTFGTGTVP
                     EEELIAAVRENFDLRPAGIIEMLDLRRPIYKQTAAYGHFGRTDVDLPWEHTDKVDALK
                     ASLAK"
     gene            3826..5298
                     /locus_tag="SCJ86_001417"
     CDS             3826..5298
                     /locus_tag="SCJ86_001417"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331067.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEG1168963.1"
                     /translation="MKKETNVTLVTVSIFIATFMTAIEGTIVTTAMPTIVGSLHGMEI
                     MNWVFSIYLLTNAMLTPIYGKLADKVGRKPIFIFGTLLFIIGSLFCGLSQSMIGLIAA
                     RAIQGMGAGAMMPVALTIIGDLYSIEKRAKVLGLNSSAWGIASVFGPLAGGFIVETIS
                     WHWIFFINVPIGILLIILIWLYLNEPKIIHESKPMDIFGSFSLMAMLVSLLLGFQLLG
                     DEGMSPLVSALLLGSIVMLGIFVFAEKRAKDPVIMMHLFKNPLFVVVNLAAALASGFL
                     MGIDVYIPMWMQGVLGLNAAIGGLVLAPLSLVWMVGSFLASKWMTQMKTHWVLRIGLL
                     IAAIGGAWLYLMPFRSNYLWFFAISTVIGIGLGVTMTTSTVSAQNSVSRDQLGVATSF
                     NTLVRTIGQTVMVSIFGLLINGVTKRELAAANLTNDADIMNKLVNPQTAKYIDADILL
                     PLRKILYDGLHSVYLVGLLLVIIALVLTLFVHEKSGKIKQ"
     gene            5381..6169
                     /locus_tag="SCJ86_001418"
     CDS             5381..6169
                     /locus_tag="SCJ86_001418"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344918.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="esterase family protein"
                     /protein_id="HEG1168964.1"
                     /translation="MAFIQANIYSNVLEMEVNLNVILPQTTKKVIGTSSEQETTDIPV
                     LYLLHGMGGNHSVWERRTSIERYVANYGLAVIMPSTDLGWYTDTVYDMKYWTFIAEEL
                     PEICHELFPQLTRKREKTYAAGLSMGGYGALKLGLAKSENFAAVISLSGAVSVGNRMD
                     DLLMVRKASFWEGIFGPLDQIEGSKNDPQYLLKELVKSGKPVPRFYMACGESDFLYQA
                     NQEMVSAMQEQGLAVTFEQGPGDHNWIFWDQWIQRALAWLMEEK"
     gene            complement(6236..6904)
                     /locus_tag="SCJ86_001419"
     CDS             complement(6236..6904)
                     /locus_tag="SCJ86_001419"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002285876.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator
                     C-terminal domain-containing protein"
                     /protein_id="HEG1168965.1"
                     /translation="MEIKLSQAIIIQAAFDILAETQALSQLSMRNIANRIHVQAPALY
                     WYFKNKQQLLQIMAETMEEELALPDPTLPWNEQLLQYMENYYDLYTRFPCGAELEINT
                     VPAFPSRLEHLEQMIQLLVREGFSVTQSHQAIASLHNLLIGQLMDQQHEQQLRQKIMD
                     GNEFLKETVLFMRNYVVENQLEGFKESMQSHAELNEKALFLTSAQTYIEGLCQQNKRR
                     ENEQ"
     gene            7019..8731
                     /locus_tag="SCJ86_001420"
     CDS             7019..8731
                     /locus_tag="SCJ86_001420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEG1168966.1"
                     /translation="MKLILRHAKKYKVAVFISLLSVAVMVAAALWQPKLLQQVLEAII
                     TEDNDEMKTIGIYLISLALLGLAAGVTNTIFSAKVAQGVSADIREEAFRKIQTFSFGN
                     IEQFSAGNLVVRLTNDITQIQNLVMISLQSLFRIPFLFIGAFILAMITMPQLWWIIVA
                     LIVTVFLITALSFTRMGKHFMIIQKLIDRVNSIAKENLLGIRVVKSFVQEENQLKSFS
                     KVSEDLTKHNIIVGTLFSVMIPSFMLAANLAVVGAIFFVSDLVKDDPTLIGGIASFMN
                     YLMQIMMAIIIGGMMMMMTSRAAVSLKRIAEILDAEPDLTYLDVPEQELTGSVTFDHV
                     SFRYPGEDTDTLKDISFSIKPGEMVGIVGATGAGKSTLAQLIPRLFDPTEGKVEVGGV
                     DLRQVNEKSLRQTVSFVLQKAILFSGTIAQNLRQGKKNATEKEMEHASSIAQAKEFIE
                     KLSDRYEAPIEERSSNFSGGQKQRLSITRGVIGNPKILILDDSTSALDARSEKLVREA
                     LDRDLKGTTTIVIAQKIASVVKADRILVLDEGRLVGEGTHEELVAGNRVYQEIFETQK
                     GTEE"
     gene            8734..10503
                     /locus_tag="SCJ86_001421"
     CDS             8734..10503
                     /locus_tag="SCJ86_001421"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010762530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEG1168967.1"
                     /translation="MTDLIKASKFFYHYLKRYKLSFFFIFITVIIATYLQVKAPQYIG
                     EAFQELANYVGGLMQGVDDKSKFVSVIWKLLLFYVLASAANFIYSILFTQVVGKSTNR
                     MRIGLFNKLEKLTIRFFDSHQDGEILSRFTSDLDNIQNSLNQALLQVITNAVLLVGIL
                     IMMFRQNVELAWATIASTPVAVLIAVLIIRKARKYVDLQQDEVGKLNGYMDEKISGQR
                     VIITNGLQEETIEGFLEHNQSVREATFKGQAYSGLLFPMMQGMSLVNTAIVIFFGGWL
                     ALNGDLERSVALGLVVTFVQYSQQYYQPLMQISSGYSMIQLAITGARRLNEIFDEKDE
                     VKPENCKAFDGVEKGLALQHVDFGYSPEKLVLKDVSINVDKGEMVALVGPTGSGKTTI
                     MNLLNRFYDVNDGSVVIDGIDIRDYDLDALRRHVGIVLQESVLFSGTIKDNITFGKPD
                     ASDEEVINAAKQANIHDFIMTLEKGYDTEISEENNLFSTGQKQLISIARTIITNPSLL
                     ILDEATSNVDTVTEARIQKAMDEAIKGRTSFVIAHRLKTILNADRIVVLRDGEVIEEG
                     SHHELLAENGFYAELYHNQFVFE"
     gene            complement(10708..11274)
                     /locus_tag="SCJ86_001422"
     CDS             complement(10708..11274)
                     /locus_tag="SCJ86_001422"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736967.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HEG1168968.1"
                     /translation="MLQTALHFSHTLLQEILQPGDHVVDATMGNGHDTVFLAEHIGKT
                     GHVYSFDIQQQAIDATRERLEQRQLEERVSLFLQGHETLGEVIAEQQNLKAGIFNLGY
                     LPKSDKAIITMPETTRTAMEEILKRLVPRGRLILVVYYGHEGGEKELDMVDSFCSKLP
                     QETYNVLNYRFINQKNQPPILYCIEKKR"
     gene            complement(11274..12230)
                     /locus_tag="SCJ86_001423"
     CDS             complement(11274..12230)
                     /locus_tag="SCJ86_001423"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002390904.1"
                     /note="This family includes YhcC from E. coli K-12, an
                     uncharacterized radical SAM protein; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR01212 family radical SAM protein"
                     /protein_id="HEG1168969.1"
                     /translation="MTFPYSEGNKRYHSWNYALRNEFGGKIFKVPIDGGFDCPNRDGT
                     VAHGGCTFCSVSGSGDMIVAPEDPLPIQFQKEIDMMHQKWPHVQQYIVYFQNFTNTHA
                     PVEIIKERFEQVVNLPGVVGLSVGTRPDCLPDEVVDYLAELNERMYLWVELGLQTTFE
                     ETSKLINRAHDYQTYLDGVAKLRKHNIRVCTHLINGLPGESLEMMKENVRRTILDSDI
                     QGIKLHLMHLMRKTRMLRDYHEGRLQLMSRPDYVNVICDQLEMIPKEIIIHRLTGDAP
                     WDSLIGPMWSLKKWEVLNAIDEELLRRDSFQGKYDVRKKVSV"
     gene            complement(12230..12886)
                     /locus_tag="SCJ86_001424"
     CDS             complement(12230..12886)
                     /locus_tag="SCJ86_001424"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318868.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatase PAP2 family protein"
                     /protein_id="HEG1168970.1"
                     /translation="MKYKTSFQVAGSFFLVLFFILGWIVKFHLSWLSGFDQWFTGIVR
                     ASYPDLNSYQLFMTKFGNPLTVIILFACAALWMWYKKKRAETFWFVSGFIVIAGIINP
                     VIKLFFMRERPSLAHLVVETSYSFPSGHAAGSMILYGSLLFLLPAFVQKRTLRTLLQL
                     LMMFIIVSVGISRIYLGVHFPTDIIGGYLLSLSWLCLTYPVYREKRKQIDNSYRKKDF
                     "
     gene            13256..15274
                     /locus_tag="SCJ86_001425"
     CDS             13256..15274
                     /locus_tag="SCJ86_001425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736969.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysM peptidoglycan-binding domain-containing
                     protein"
                     /protein_id="HEG1168971.1"
                     /translation="MENIARKERRRLEQTKRFRKAKRGAAIVGTAMVGCSVAAPLVQP
                     VPVQADETPVQFGARINTAAFITEIATYAQPIAQANDLYASVMIAQAVVESGWGSSAL
                     SQAPYYNLFGIKGSYQGQTVYMDTLEFLNNQWVNKKEPFRQYPSFAESFNDNAYVLRN
                     TSFGNGYYYAGTWKSNTKSYTDATACLTGRYATDPGYAGKLNNIIATYGLTQYDTPAS
                     GNAGGGATTGGNNTGSAGSTGGSSNSGNTNSGSATSTTYTVQSGDSVWGISNKFGITM
                     DQLIQWNNIQNNFIYPGQKLTIKGSQENGSSTNNSGNNTNSSGNAGTSNGGQTTGAKY
                     TVQSGDSVWKISNDHGITMAQLIEWNNIKNNFVYPGQQLIVSNGGSSNAGANTGSNTG
                     NTSGSSSSSSSSSNTASGTKYTVKAGDSVWSVSNKYGITMAQLIQWNNIQNNFIYPGQ
                     QLTVSNSGSTNSTSGNTGSSTNTNNSSSVNTGSSSSQGTYTVKAGESVWSVANKNGIT
                     MDQLIEWNNIKNNFIYPGQQLIVKGGTSANTNTGSTTSAAKPNTPNTSATTSTSSTGN
                     TMYTVKAGESVWSVANKHHITMDQLIEWNNIKNNFIYPGQQVIVKKGSSQSTNQQSVT
                     GSKTYTVKSGESVWSVADSHGITMAQLIEWNNIKNNFIYPGQTLIVAK"
     misc_binding    15344..15550
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00230"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="T-box leader; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="tRNA"
                     /db_xref="RFAM:RF00230"
     gene            15701..18115
                     /locus_tag="SCJ86_001426"
     CDS             15701..18115
                     /locus_tag="SCJ86_001426"
                     /EC_number="6.1.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019722570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="leucine--tRNA ligase"
                     /protein_id="HEG1168972.1"
                     /translation="MSYNHKEIEKKWQKYWAKTNTFNTHDDPEKPKFYALDMFPYPSG
                     QGLHVGHPEGYTATDILSRVKRSQGYNVLHPMGWDAFGLPAEQYALDTGNDPAEFTQK
                     NIETFRRQINSLGFSYDWNREVNTTDPEYYKWTQWIFTKLYEKGLAYEAEVAVNWVPE
                     LGTVISNEEVIDGKSERGGYDVVRKPMRQWMLKITAYADRLLDDLELVDWPESIKEMQ
                     RNWIGRSVGANIEFKVAGTDKSYTVFTTRPDTLFGATYSVLAPELDLVREITTPEQKA
                     AVEAYIEETAKKSDLKRTDLAKEKTGVFTGAYAINPVNGKEIPIWIADYVLSSYGTGA
                     IMAVPAHDERDFEFAQTFGLEILPVIEGGDVEKAAYTEDGTHINSEFLDGMNKQEAID
                     KMNEWLEENGVGKKEVSYRLRDWLFSRQRYWGEPIPIIHWEDGTVTAVPEEELPLRLP
                     KTKNIKPSGTGESPLANIEEWVNVVDPVTGKKGRRETNTMPQWAGSSWYYLRYIDPHN
                     KKELADYEKLKRWLPVDIYIGGAEHAVLHLLYARFWHKFLYDIGVVPTKEPFQKLYNQ
                     GMILGENNEKMSKSRGNVVNPDDVVETYGADTLRMYEMFMGPLDASIAWSENGLEGSR
                     KFLDRVWRLIVDENNKMRDRITTLNDGKLDKVYHQTVKKVTEDYENLHFNTAISQLMV
                     FINEAYKVDALPYEYIEGFVQLLAPIAPHMGEELWAILGNDGGISYAPWPTYDEAALV
                     EDEVEVVFQVNGKVRAKSNVPRDLGKDELEKAALANEIVQEYIEGKTVRKVIAVPNKL
                     VNIVAN"
     gene            18463..20016
                     /locus_tag="SCJ86_001427"
     CDS             18463..20016
                     /locus_tag="SCJ86_001427"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327332.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyl hydrolase"
                     /protein_id="HEG1168973.1"
                     /translation="MDQELFIIEVLSAKQKIIEKAKKRAKRIMFKLLSLVFFSGLLFF
                     GAVSFLYRHMSAVVRSAEGMQKLNNLYHFSWGVGGITIVVLIVFLLLIWLRYHQLSRH
                     FDQWLSQRAQQFLHNKPTSQDDDYLYFEKTSTSQAFRIQKSDCQLLLRSLDDLPIYLG
                     ESKSKFGMNTFHIFLLENEPRWEKSQSSPGGYLTTKMSTLVTGFFLILLAGTIYYGRY
                     TQPKMSSHINAASHMTMNSTNDTHIQKTNEGLLADPKQKVNLTTQKINDLKLDTETHS
                     LYMTTDSGANWVFVPIDSQWLRFGEYTLTTGLIPEGYWMDNTYDISADFSWFIYSSGD
                     DVRLLTSRDNGKTWQTSIITQSVQPIRYRKAVFYGNDGLFICTSASGMSAETLYIYTT
                     MDGGITWIKSGSTMINQPVQNVSFVTTSLGFVSTRESLFYTNNGGRSFKESVLSIPEN
                     YQTGGIDLFQSPNEVTQISANTLETKFYLLKKGPIDAGKMFACLFRSTDNGETWQFVQ
                     QLSQVEQGD"
     gene            complement(20397..22181)
                     /locus_tag="SCJ86_001428"
     CDS             complement(20397..22181)
                     /locus_tag="SCJ86_001428"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEG1168974.1"
                     /translation="MEKKKTLNTRALIRFWKMIKPEHKYFYGLFLCSLFGNLLVVAMP
                     LIMGIGIDDLLSEIRKTGIGQLSFSDIKQALLFPVLLLIGCSVLSSITSFIQERTMAA
                     LSEKITLRVRKEITKKFKTLPMSFFDNHQVGDIISRTTTGLNQLSQVLLTGINQFFTS
                     VVTIAAAGLMLFYIESKLTLLVLLLIVGSSIVTTKIANKNKQISEQNQYELGVLNNRM
                     EEILAGNLVVKTFNQQAHAEAAIHEVNKKQYNAFKRAQFMNCAIYPAIRFINQLAFII
                     SAVFGAMLVLSGGVTIGFLQAYLQYINQISEPISTASYVINSIQSAMAAIDRIFEILD
                     ETDEIPDLPDARLLEQPKGKIAFKNVQFGYTSDKILMNQVDFSVKPKQTVAIVGPTGA
                     GKTTLVNLLMRFYELNKGKIEFDGLDITKLSRGELRRSFGMVLQNTWLFEGTVADNIA
                     YGKMDATREQIIEAAEIAQCDHFIRTLPDGYDTIISSENGTLSQGQQQLLTIARVILA
                     NPPVVILDEATSSVDTRTEALIQQAMEAVTKDRTSFVIAHRLSTIENADMILVMKNGD
                     IVEKGTHSELLQSPTLYASLYNSQFQIA"
     gene            complement(22196..23917)
                     /locus_tag="SCJ86_001429"
     CDS             complement(22196..23917)
                     /locus_tag="SCJ86_001429"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313055.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEG1168975.1"
                     /translation="MSILKTFLQKNKGLALLTFVMICLEIAGTLGVPKLVAKLIDTGI
                     ASGEASVIKKIGVEMLLVAFLGTIAAVASSYFSALASARFGFQTREKYFRKFQQLSMK
                     DISRFSSGSLLTRMTNDVDNVQQMIVLFCQMILPAPVICVFTILMMFRYSLLLTWVTL
                     ISVVFYVWIVYRLMKRGAPLSLSIQPKMDRITITLREFFTGINMIRAFDNQSYEEKRT
                     NKSFADYANQMIKVNRIFAWVTPIAFLLMGIVYASILWFGGNLVAEGALQIGVVTAVV
                     EYSMLTLAYLMIAAMVLVIIPKSFASLRRIEEILQAEIEIEEETIKTVAEPTFDQTNA
                     AAFEHVTFKYTEISDPVLEDIDFTIPKGKTTAIVGGTGSGKSSLAKLLLRLSDPTIGK
                     VTLGGIPLTQMTQEQIRSHISYVPQKAFLFSGTILSNLQMGNEYASTEELSKAIKIAQ
                     LEELVDTLPDGLDSFVAQGGSNYSGGQKQRICIARALIKPAEIYVFDDSFSALDYKTD
                     AALRAALHKHMSEKTLVIVAQRLSTIQQADNIIVLDEGKIVGQGTHEELLSNCQTYQE
                     FAKSQGI"
     gene            24017..24778
                     /locus_tag="SCJ86_001430"
     CDS             24017..24778
                     /locus_tag="SCJ86_001430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330958.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
                     /protein_id="HEG1168976.1"
                     /translation="MKKMYTIGQVSELFDLPKSTIRYWDEQGLIRSSRQEENDYRLFD
                     IDDIFMLYDIVFYRKLDIPIKQMKNLYGKTLTELYETLDETERRIHKELVVMKQKQKE
                     IREQKKQLKLMIDTNEEEFPVEEIPFDCMISTEFEDIVEIKKFLPNYSSFGMMSMPAS
                     SSQTMYGFFIDPSEVHLFTQDVIWEKKDTAVYRRFLLKSEMNHAERNNILEIRERMYE
                     KGWKTGEVIGQYLLTNTDENNIRTEYYHAWIEMKK"
     gene            25745..26013
                     /locus_tag="SCJ86_001431"
                     /pseudo
     CDS             25745..26013
                     /locus_tag="SCJ86_001431"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013720767.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS607 family transposase"
     gene            26685..>28861
                     /locus_tag="SCJ86_001432"
     CDS             26685..>28861
                     /locus_tag="SCJ86_001432"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016922466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEG1168977.1"
                     /translation="MRNKFHKLLSMFLVMLIVINQFGNITVLAESLAEQPERSLTIEV
                     DQEEAALGEQFQLRVRGTPDLLNALVIDKSEDLIEGEVKQLNDYERLITYSSQHSGKY
                     ELTARSGDIQSNTVSIEISVPEESTSESFNVPEESTSESFNIPEESLSESFNEARSSE
                     PSITQESQIERQEELLNYDIGISAERPTLPSRHTQTLKGYIQGGAGDILEEYGEMVVR
                     VPRASIGLIEFIRVEVADDSPLKPLAFYQEGTDVVFPFTFDYEGLNEAAQSYTFSITF
                     EVAFFQSGGTEPNNDFQVSLTYAERAANTEFSITPIPSSRPIFSKLYYGSREIDSQGN
                     LGNLLIDGQDVTNSNKFILQVNYEGSRKDGVVVTDVLPQGTTLAPNPTTWSSTGNREP
                     ISGGVRIAEVTNPGEANVRTHYVSEEFVDKVSFNKETNTLVVDFGNLAEGQSYFIEYA
                     LEVTDVDLFNEGRQEDGFSRNRASMTYNNQVMSRDYRLRIPESNLPAISLRKSVDKNV
                     INLNENELTYTLHVSMFSGTRNTLVIEDVLEEGMEFVSFLSDIPSFIDAEVNNGIVRF
                     SANNTIGGPTSFTLSFKVNVEAYAVGDQILNFATLVNSDNRLNTSTVRTRKIDGRIKV
                     IKTDENGQRLAGAKFEILSSDSQVIQEGITAENGEFLSEPLPVGSYQVRETEAPEGYL
                     LDATVHDVFINEYSIEEVTLTIENILDTGGVELIKHAEGSGEVL"
BASE COUNT         9039 a         5194 c         6149 g         8479 t
ORIGIN      
        1 aagttatcca tggaggttta gctcagctgg gagagcatct gccttacaag cagagggtca
       61 gcggttcgat cccgttaacc tccatatgag ccgttagctc agttggtaga gcatctgact
      121 tttaatcaga gggtcacagg ttcgagccct gtacggctca tatttttgcg ggtgtggcgg
      181 aattggcaga cgcactagat ttaggatcta gcgccttacg gcgtgggggt tcaagtccct
      241 tcacccgcat aatttaagtt ttacatgccg gcttagctca gttggtagag catctgattt
      301 gtaatcagag ggtcgagggt tcaagtcctt tagccggcat ctttaaacac gcggaaatag
      361 ctcagtggta gagcaccacc ttgccaaggt gggggtcgcg ggttcgaacc ccgttttccg
      421 ctttgccaaa gcaatcgcgc cggggtggcg gaactggcag acgcacagga cttaaaatcc
      481 tgcggtgagt gatcaccgta ccggttcgat tccggtcctc ggcatttcaa atatgcgccc
      541 atagctcaac tggatagagt gtttgactac ggatcaaaag gttaggggtt cgactcctct
      601 tgggcgcgtt aattaaacgg gaagtagctc agcttggtag agcacttggt ttgggaccaa
      661 ggggtcgcag gttcgaatcc tgtcttcccg ataattataa atttaaacac ggggccttag
      721 ctcagctggg agagcgcctg ctttgcacgc aggaggtcag cggttcgatc ccgctaggct
      781 ccatttcatt taaataatga atatcttttt cgcggtgtag ctcagctggc tagagcgtcc
      841 ggttcatacc cgggaggtcg ggggttcgat cccctccgcc gcgattttta agagcctgga
      901 cctttagctc agttggttag agcagacggc tcataaccgt ccggtcgtag gttcgagtcc
      961 tacaaggtcc attgtggcgt tgtaagtttc atatggagga ttacccaagt ccggctgaag
     1021 ggaacggtct tgaaaaccgt caggcgggta aaaccgtgca agggttcgaa tcccttatcc
     1081 tcctttttca tattatcgcg ggatggagca gtctggtagc tcgtcgggct cataacccga
     1141 aggtcgtagg ttcaaatcct gctcccgcaa ttaaaaacta aaaacactgg ttccgtggtg
     1201 taggggttaa catgcctgcc tgtcacgcag gagatcgcgg gttcgattcc cgtcggaacc
     1261 gccatgcggg tgtagtttag tggtaaaacc acagccttcc aagctgttgt cgcgagttcg
     1321 attctcgtca cccgctttta gttttttatg ggcctatagc tcagctggtt agagcgcacg
     1381 cctgataagc gtgaggtcga tggttcgagt ccatttaggc ccattagtac aaaatcttat
     1441 tggttttggg gaagtactca agtggctgaa gaggcgcccc tgctaagggt gtaggtcggg
     1501 aaaccggcgc gagggttcaa atccctcctt ctccgtttca atattttgtt ttacctggcc
     1561 cgttggtcaa gcggttaaga caccgccctt tcacggcggt aacacgggtt cgaatcccgt
     1621 acgggtcatt aatttaatat agttttacat ggaggattac ccaagtccgg ctgaagggaa
     1681 cggtcttgaa aaccgtcagg cgggtaaaac cgtgcaaggg ttcgaatccc ttatcctcct
     1741 ttttcatatt atcgcgggat ggagcagtct ggtagctcgt cgggctcata acccgaaggt
     1801 cgtaggttca aatcctgctc ccgcaattaa aaactaaaaa cattggttcc gtggtgtagg
     1861 ggttaacatg cctgcctgtc acgcaggaga tcgcgggttc gattcccgtc ggaaccgttt
     1921 ggctcggtag ctcagttggt agagcaatgg attgaagctc catgtgtcgg cagttcgatt
     1981 ctgtcccgcg ccattatgcg ggtgtagttt agtggtaaaa ccacagcctt ccaagctgtt
     2041 gtcgcgagtt cgattctcgt cacccgcttt tagtttttat gggcctatag ctcagctggt
     2101 tagagcgcac gcctgataag cgtgaggtcg atggttcgag tccatttagg cccatttatt
     2161 ttattggccc gttggtcaag cggttaagac accgcccttt cacggcggta acacgggttc
     2221 gaatcccgta cgggtcatgg aagatacttt ttgagtatct ttttcttttt atctgaaaga
     2281 atgattttaa aaagcttagc agagttatct ttatggatga ttgtgctaag cttttttgga
     2341 cagttctaat gtaaaatatt tttatatttt ttaagcaaaa aaatgaaaaa atacgagcag
     2401 aaaactgttt tatctgctat acttggttcg ttgcaagttc ccgaaaggat ccgcaggacg
     2461 gagatgcctt gtaaccgaat ttaactaggg ggaatcatta taatggtaga aagacactta
     2521 tttacatcag aatctgtttc tgaaggacat ccagataaaa ttgctgacca aatcagtgat
     2581 gcaatcttgg atgcaatttt aaaacaagat ccaacagcac gagtagcgtg tgaaacgtct
     2641 gtaacaactg gtcttgtttt agtatttgga gaaatatcta cgacggcata tgtagatatt
     2701 caaaaagtcg tacgggaaac aataaaagaa attggttata cacgtgcaaa attcggattt
     2761 gatggagata cagcagctgt attggttgcg attgatgaac aatctcctga tattgctcaa
     2821 ggagttgatg aagcacttga gatccgggat gaagataaaa aagacgtatt agatgagatt
     2881 ggtgctggtg accaaggttt aatgtttggg tttgctgtag atgaaacacc agaattgatg
     2941 cctttaccaa tcgctttgag tcatcgtctg gtacgacgct tggcagattt gcgtaaatca
     3001 aatgaattaa cgtatttacg tccagatgca aaatctcaag taacggttga atatgatgat
     3061 caaggacaac cggaacgcgt agatacaatc gttatttcaa cacagcatga tgatgcagta
     3121 gataatgaaa caattcgtca tgatgtcatc gaaaaagtag tgaaggaagt tattccagct
     3181 gaattattag atgatcaaac gaaatattat atcaatccga ctggccgatt tgtcattggt
     3241 ggtcctcaag gggatgccgg attaacagga agaaaaatca ttgttgatac gtatggcggt
     3301 tatgctcgtc atggtggcgg tgctttttct ggtaaagatg cgacaaaagt tgaccgttct
     3361 gccagctatg ctgcacgtta tattgctaaa aacattgttg cagcagggct tgctcgcaaa
     3421 gcagaagtac agctagctta tgcgattggt gttgctcaac ctgtttcgat ctcaatcaat
     3481 actttcggta ctggaacggt tccggaagaa gaactgattg ctgcagtgag agaaaacttc
     3541 gatcttcggc cagcaggaat catcgagatg cttgatttgc gtcgtccgat ttacaaacaa
     3601 acagctgctt acggtcattt tggtcgtaca gatgtagatt tgccttggga acatacggac
     3661 aaagtagatg cgttgaaagc aagtttagca aaataagtta tattcagctg gaacgatcgc
     3721 caagtggtgg ttgttccagc tttttttatg gcagttaacc tcttgaaaaa gatagtaatt
     3781 atttatctat ttttagatcc tgtcataatg aaagggagga ataagatgaa aaaagaaaca
     3841 aatgttactt tggtcacagt gagtattttc attgcaacgt tcatgacggc tattgaaggt
     3901 acaatcgtaa caaccgctat gccaacgatt gtcggttcat tacatggaat ggaaatcatg
     3961 aactgggtat tttcgattta tttgctgaca aacgctatgc ttactccgat ttacggaaaa
     4021 cttgcggata aagtgggacg gaaaccaatc tttatttttg ggacattatt gtttattatc
     4081 ggttcgttat tttgcggttt atctcaaagc atgattgggt tgattgctgc tcgtgcgatt
     4141 caagggatgg gtgctggggc aatgatgcca gtagctttga cgatcatcgg cgatctttat
     4201 tctattgaaa agcgagcaaa agttttaggt ttgaatagtt cagcctgggg gattgccagt
     4261 gtttttggac cgcttgcggg aggattcatc gttgaaacga tcagctggca ctggatcttt
     4321 tttatcaatg tgccgatcgg aattctatta attattttga tttggctata tttaaatgaa
     4381 ccaaaaatca tacatgagtc taaaccaatg gatatttttg gtagttttag tttgatggcc
     4441 atgcttgtct cattgctgtt agggttccaa ttattaggag acgaaggaat gagtccgctt
     4501 gtttctgctt tgctgctagg tagtatcgta atgttaggta tttttgtttt tgccgaaaaa
     4561 agagctaaag atcctgtcat catgatgcat ttattcaaaa atccactatt tgttgtggtc
     4621 aacttggctg cagcattggc tagtggtttt ttgatgggaa tcgatgttta tatccctatg
     4681 tggatgcaag gtgtattagg gctgaatgca gcaattggtg ggctggtatt agcaccatta
     4741 tcactagtct ggatggtcgg ttctttcctt gcaagcaagt ggatgactca aatgaaaact
     4801 cattgggtct tgagaattgg actactaatc gcagctattg ggggcgcttg gttgtatctt
     4861 atgcctttca gaagtaacta tttatggttt tttgctatat caacggtcat cggtataggc
     4921 ttaggtgtga ccatgactac ctcgactgtc agtgcacaaa atagtgtatc tcgtgaccag
     4981 cttggtgtag caacttcttt taatacgtta gttcgaacaa ttggtcaaac agtcatggtt
     5041 tctatttttg gtttattgat aaatggggta actaaacgag aacttgcagc agcaaattta
     5101 acaaatgatg cagatatcat gaataaactg gtgaatccac aaacggctaa atatattgat
     5161 gcggatattt tgttaccatt aagaaaaatt ctttatgatg ggttacattc cgtttatcta
     5221 gttggcttgt tactcgtgat cattgctttg gtcttaacgc ttttcgttca tgaaaaaagt
     5281 ggtaaaataa aacagtagtt tcggttaaga tatgtacgta gccgaatcat tcaaatttaa
     5341 agttattcaa aaataacttc ttactaagga aggcgaaaat atggcattta ttcaagcaaa
     5401 tatttattcg aatgtgttag aaatggaagt caatctaaat gtgatactac cgcaaacaac
     5461 aaaaaaggtg attggcacca gttcagagca agagacaacg gatatacctg ttttatattt
     5521 acttcatgga atgggtggca atcatagtgt atgggaaaga cgtacgtcga ttgaacgtta
     5581 tgtagcaaac tacggattag ctgtcatcat gccatctact gatcttggct ggtacacaga
     5641 tacagtttat gacatgaagt attggacatt cattgcagaa gagctacctg agatttgtca
     5701 tgaattattc ccgcaattaa caagaaaaag agaaaaaaca tatgccgctg gattatcgat
     5761 gggcggatat ggcgcgttaa aactaggcct tgcaaaatca gaaaatttcg cagcagtaat
     5821 ctctctttca ggcgctgttt ctgtaggaaa tcgaatggat gatcttttga tggttcgtaa
     5881 agcatctttt tgggaaggga tctttggacc attggatcaa atcgaaggtt caaaaaatga
     5941 tccgcaatac ttattgaaag aactagtaaa aagcggaaaa ccagttccaa gattttatat
     6001 ggcttgtggg gaatctgatt ttctttatca ggcaaatcag gaaatggtat ctgctatgca
     6061 ggaacaaggc ttggcggtca cctttgaaca aggacctggc gatcataact ggatcttctg
     6121 ggaccaatgg attcaacgtg cattggcgtg gctaatggaa gaaaaataaa aaaagagtct
     6181 taaaaagata gctcctatag agatcacaaa catagtgatc tctataggag cttttttact
     6241 gctcgttttc tcttctttta ttttgttggc ataagccttc aatataggtt tgtgcactcg
     6301 ttagaaagag tgctttttca ttcaattctg catgggactg catactttct ttaaatccct
     6361 ctaattgatt ttcaactaca taattacgca taaatagaac tgtttctttc aaaaattcat
     6421 ttccgtccat aattttttgt cgaagctgct gttcatgctg ctgatccatt aactgtccaa
     6481 tcaataaatt gtggagagag gcaatggcct gatgactttg ggtgactgaa aatccttcac
     6541 gaacaagtaa ttggatcatt tgctccaaat gttctaatcg actcggaaaa gcgggcacag
     6601 tattgatttc taattctgcc ccacaaggga aacgtgtata aaggtcatag taattttcca
     6661 tatactgcag caactgttca ttccaaggca aagttggatc tggaagcgct aattcttctt
     6721 ccattgtttc agccattatt tgcaagagct gttgtttgtt tttgaaatac caatataaag
     6781 caggtgcttg aacatgaatt ctgtttgcga tgtttcgcat ggaaagctgc gaaagagctt
     6841 gtgtttcagc caggatatca aaggcggcct gaatgatgat tgcttgagaa agctttattt
     6901 ccattttatc aacctcgtgt attgcattat ctttttaaac atggtaaatt gttagtcggt
     6961 ctaattttac agtgttaaat tagtcctgtc aatttaaaca aaggggtgta atcaacgaat
     7021 gaaattaatc ttacgacatg cgaaaaaata taaagtagct gtttttattt ccttgctgtc
     7081 cgtagcagtc atggttgcag cagcattatg gcaacctaag ttactacagc aagtattgga
     7141 agctattatt acagaagata atgatgaaat gaaaacaatt ggtatttacc tgatcagtct
     7201 ggctttgcta ggattagccg caggtgtaac gaacacgatt ttctctgcaa aagtagctca
     7261 aggcgtaagt gcagatatcc gagaagaagc atttcgaaag atccagacat tttcatttgg
     7321 aaatatcgaa cagttctctg ctggaaatct tgttgttcgt ctaacaaatg atatcacgca
     7381 aatccaaaat ttagtgatga tttctttgca atcacttttt cgaattccat tcttatttat
     7441 tggtgccttc attttagcta tgatcacgat gccgcaatta tggtggatca tcgtagcgct
     7501 tatcgttacg gtatttctga ttacggcact ttcatttaca aggatgggca agcacttcat
     7561 gatcattcaa aaactgattg atcgtgtgaa tagcattgca aaagaaaact tactagggat
     7621 tcgtgtagtg aaatcttttg tacaagaaga aaaccaattg aagtcattca gcaaagtaag
     7681 tgaggactta acaaaacaca acatcatagt cggtacatta ttttctgtga tgatcccgtc
     7741 cttcatgctg gctgccaatt tggctgtggt tggggcgatc ttttttgtga gcgatcttgt
     7801 taaagatgat cctacgttaa ttggtggaat tgcttcattc atgaactact tgatgcagat
     7861 tatgatggca atcattattg gcggaatgat gatgatgatg acttcaagag cagccgtatc
     7921 gctgaagcgg attgcggaaa ttttagatgc tgaaccagat cttacttatt tagacgttcc
     7981 agaacaagag ttgacgggtt ctgtcacttt tgaccatgtt tcttttcgtt atcctggtga
     8041 ggatacagat acgcttaaag atatcagctt ttctatcaaa cctggagaaa tggttggtat
     8101 cgtcggggcg actggtgccg ggaagtcaac actagcacaa ttgatccctc gtctatttga
     8161 tccgacagaa ggcaaagttg aagtaggtgg tgtagatctt cgtcaagtaa atgaaaagag
     8221 tttaagacaa acggtttcct ttgttttgca aaaagcaatt ttattctcag gaacgattgc
     8281 tcaaaacttg cgccaaggga aaaagaatgc aacagaaaaa gagatggaac atgcttcttc
     8341 tattgctcaa gcaaaagaat tcatcgaaaa gttatctgat cgttacgaag caccaataga
     8401 agaacgtagc agtaactttt ctggtggtca aaagcaacgt ttatccatta ctcgtggcgt
     8461 tatcggtaat ccaaagatct tgatcctaga tgacagtacg agtgcactgg atgctcgctc
     8521 tgaaaagctg gtccgagaag cactcgatcg agatttgaag ggaacaacga cgattgtcat
     8581 cgcacaaaaa attgcttctg ttgtcaaagc agaccgcatc ttagtattgg atgaaggcag
     8641 attagtcggc gaaggaaccc atgaggaatt ggtagctgga aaccgtgtct atcaggaaat
     8701 ctttgaaact caaaaaggaa cggaggagta agtatgactg atctaataaa agcaagtaaa
     8761 tttttctatc actatttgaa gcggtacaaa ctctcctttt tctttatctt tatcacagtg
     8821 atcattgcta cctatctaca agtaaaggca cctcaataca tcggggaagc ttttcaagaa
     8881 ttagctaatt acgtaggagg cttgatgcaa ggcgtagacg ataaaagcaa atttgtttct
     8941 gtaatatgga aactcttgct gttttatgta ctagctagtg cagccaactt tatctatagt
     9001 attttgttca cacaagtagt agggaagtca acgaaccgaa tgcgtatcgg tctattcaac
     9061 aaattggaaa aattgacgat ccgtttcttt gattctcatc aagacggtga aattttaagt
     9121 cgttttacca gtgacttaga taatattcaa aatagtttga accaagcttt gcttcaagtc
     9181 atcacgaatg ctgttttact tgtcgggatc ttgatcatga tgttccgcca aaatgtagag
     9241 ttagcttggg ctacaattgc ttccactcca gtagccgttt tgatagctgt cttgattatc
     9301 cgcaaagcac ggaaatatgt ggatcttcag caagatgaag tggggaaact aaatggttat
     9361 atggatgaaa aaatcagcgg acaacgtgtc atcatcacaa atggcttaca agaagaaacg
     9421 attgaaggat tcttagagca taatcaaagt gttcgcgaag caaccttcaa aggacaagct
     9481 tattctggtc tactatttcc gatgatgcaa ggaatgtcac tggtcaatac agcgatagtt
     9541 atctttttcg gcggctggtt agcattaaat ggtgatttgg aacgttcggt ggcattaggc
     9601 ttagttgtga cattcgtcca gtattcacaa caatattacc aaccactgat gcagatttca
     9661 tctggataca gcatgatcca attagcgatt acaggggcaa gacgattgaa tgaaatattt
     9721 gatgaaaaag atgaagtcaa accagaaaac tgtaaagcat ttgatggtgt tgaaaaaggt
     9781 cttgcgttgc agcacgtaga ttttggttat tctccagaaa aactagtctt gaaagatgtc
     9841 tcgatcaatg tggacaaagg agaaatggtt gctcttgttg gtccaacagg atcggggaaa
     9901 acgacgatca tgaatctgct aaaccgtttt tacgatgtca atgatggttc agtagtcatc
     9961 gatggaatag atatccgaga ttatgatctt gatgcattac gtagacatgt aggaatcgtt
    10021 ttgcaagaat ctgtattgtt ctccggaacg atcaaagaca atatcacatt cggcaaaccg
    10081 gacgcttcag atgaagaagt catcaatgca gcaaaacaag cgaatattca tgacttcatc
    10141 atgacgttag aaaaaggtta tgatacagag atatccgaag aaaataactt attcagtaca
    10201 ggtcaaaagc agttgatcag tattgctcga acgatcatca caaatccatc tttattgatt
    10261 ttggatgaag caacaagtaa tgtagacacg gtaacagaag caaggatcca aaaagcaatg
    10321 gatgaagcaa tcaaaggaag aaccagcttt gtgattgctc accggctgaa aacaatcttg
    10381 aatgcagata gaatcgttgt cttgcgtgat ggggaagtca ttgaagaagg cagtcatcac
    10441 gagttattag cagaaaatgg attttatgcg gaactttatc ataatcaatt tgtatttgaa
    10501 taaagaggtt gtgaaaaagc tgtcctgcat caagtaataa gaacagccaa tcaaaaacag
    10561 cttctcatgt ttttgatttg gttggactta ttaccgcaga tataagcttt tgagcaccgt
    10621 gaataaagag gttatgaaat caacgctcta acctgagtat tagagaaaaa aacgcaaaac
    10681 atatcattta tgttttgcgt tttttattta tctttttttc tcaatacaat ataaaatagg
    10741 cggctggttc ttttggttga taaaccgata attcagcaca ttatatgttt cctgtggcag
    10801 tttggaacaa aaagaatcaa ccatatccag ttctttttct ccaccttcat gaccatagta
    10861 gacgacaagg atcaatcgtc cgcgaggcac aagtcgtttc aatatttctt ccatagctgt
    10921 gcgtgtagtc tctggcatag tgatgattgc tttatcactt tttgggagat accctagatt
    10981 gaaaatccct gctttgagat tttgttgttc agctatcact tctcctaacg tttcgtggcc
    11041 ttgtagaaac aatgagacac gttcttctaa ctgacgctgt tctagccttt cacgagtagc
    11101 atcgattgct tgttgctgga tatcaaaact atagacatga ccagtttttc cgatgtgttc
    11161 tgctagaaaa accgtatcgt ggccattgcc catcgttgca tctacgacgt gatcccctgg
    11221 ttgcaagatt tcttgcagta atgtatggct gaaatgcaaa gcagtttgca acatcagacg
    11281 ctcacctttt tccgtacatc atattttcct tggaagctat ctctgcgaag aagttcttca
    11341 tcaattgcat tcaagacttc ccattttttc aagctccaca ttggtccaat caaggaatcc
    11401 cacggcgcat cccctgtcaa acgatggatg atgatctctt ttggtatcat ttccagctgg
    11461 tcgcaaatga cattgacata gtcaggacgg ctcattaatt gaaggcgacc ttcatgatag
    11521 tcccgcaaca ttctcgtttt gcgcatcaga tgcatcaaat ggagcttgat tccttggata
    11581 tctgaatcta aaatggtgcg tctgacattt tctttcatca tttctaaact ttcgcctggt
    11641 aatccgttga tcaaatgcgt acaaacacgg atattatgtt tgcgtaattt agccacgcca
    11701 tctaaataag tttgataatc atgtgctcgg ttgatcagtt ttgaagtttc ttcaaaagtt
    11761 gtttgcaatc caagttctac ccataaatac atccgctcgt tcagttcagc aaggtaatca
    11821 actacttcat ctggcagaca atccggtcgt gtaccgactg ataatcctac gacacctggt
    11881 aaattgacta cttgctcaaa tcgttctttg attatttcaa ctggcgcatg ggtgtttgta
    11941 aaattttgga aataaacgat gtattgttgg acatgaggcc atttttgatg catcatgtcg
    12001 atttctttct ggaattggat tggaagaggg tcttcaggtg caacgatcat gtctcccgaa
    12061 ccagatacac tgcaaaaagt acaaccgcca tgggcaacag tcccatctcg attcggacag
    12121 tcaaatcctc catcgatcgg caccttgaaa attttcccgc caaactcatt tcgtaaagcg
    12181 taattccaag aatgatatct tttatttcct tctgaataag gaaaagtcat taaaagtcct
    12241 tctttctgta agagttatct atttgttttc ttttttctct atacactgga taagtcaggc
    12301 acagccaaga aagactgagt aagtaaccgc cgatgatatc agtaggaaaa tgaacgccca
    12361 gataaatgcg gcttatcccg acacttacta taataaacat catcagcaat tgaagcaatg
    12421 tgcgtaaggt tcttttttga acaaaagctg gaagcagaaa tagtaaagaa ccgtaaagaa
    12481 tcatactgcc tgctgcatgt ccactaggga aactgtaact tgtttcaact actaagtgcg
    12541 ccaatgaagg acgctctcgc atgaaaaata gtttgatcac cgggttgatg attccagcaa
    12601 ttacaataaa tccagaaacg aaccaaaatg tttctgccct ttttttctta taccacatcc
    12661 ataaagccgc acaagcaaat aaaatgatga cggtaagagg attaccgaat ttagtcataa
    12721 agagttggta gctgttcaga tcaggataag aagctctgac aatgcctgta aaccactgat
    12781 cgaaaccaga aagccacgac agatggaact ttacgatcca gcccaaaatg aaaaacagga
    12841 caagaaagaa gctaccagcc acctgaaatg atgttttata tttcatacaa ttcctccttt
    12901 gaagcaatga cttattatag catttttggt ccagcgaaaa aaacggtaaa gagataaaac
    12961 caatgtatga ttcctctttg aataaccaat attcttataa aaaaaataac tgtaatcgga
    13021 aagacatttt ttggtaatgc aaatgaaatg ttagggaaat aaaatcagtg tttttcgaaa
    13081 tccttttaaa caagaacgta gacgtaggaa gaacttgaaa atgaacagaa gttgttcatt
    13141 acggtaaatt acaatatgat tacaaaaaac aacaaagctc ttaaataact tttaataaat
    13201 attaagagaa gttataatta ttaataatag ataaattgaa gttggaggat agaagatgga
    13261 aaacattgca agaaaagaac ggagacgttt agaacagacc aaacgttttc gaaaagcaaa
    13321 acgaggggca gcaatcgtgg gaactgcgat ggtaggctgt tctgtcgctg ccccattagt
    13381 acagccggtt cctgttcaag cggatgaaac acctgttcag ttcggcgcaa gaatcaatac
    13441 agcggcattt atcacagaaa ttgctactta tgctcaacca atcgctcaag ccaacgatct
    13501 atatgcatca gtgatgatcg cacaagcagt tgtagaaagc ggttggggca gtagtgcgtt
    13561 atctcaagca ccttattata atctttttgg gataaaaggc agctatcaag gacaaaccgt
    13621 ctacatggat acgcttgaat ttttaaataa tcaatgggta aataaaaagg agcctttccg
    13681 acaatatcct tcttttgctg aatctttcaa tgacaatgcc tatgtactta gaaatacttc
    13741 atttggcaac ggttattact atgccggtac ttggaagagc aacacgaaat cctatacaga
    13801 tgcgacagct tgtttgacag ggcgttatgc gactgatcct ggttacgcag gcaaattaaa
    13861 taatatcatc gcaacttacg gattgaccca atatgataca ccagctagcg gaaacgcagg
    13921 cggtggagcg actacaggtg gcaataatac tggaagtgca ggtagtactg gcggttcaag
    13981 caattctggt aatacaaact caggttcagc aacttcgacc acttatactg tccaatctgg
    14041 tgattctgtt tggggaatct caaataaatt cggtatcaca atggatcaat tgatccaatg
    14101 gaacaacatc caaaacaatt tcatttatcc aggacaaaaa ctgacaatca aaggtagcca
    14161 ggaaaatggc tcttcgacga ataattccgg taacaatacc aattcttcag gaaatgctgg
    14221 tacatcaaac ggaggacaaa caactggtgc gaaatacact gttcaatctg gtgattctgt
    14281 ttggaagatc tcaaatgatc acggaattac gatggctcaa ttgattgaat ggaacaatat
    14341 caagaacaat ttcgtttatc caggacaaca gctaatcgta tcaaatggcg gttcttctaa
    14401 tgctggagca aatactggct caaatactgg gaatacaagt ggctctagtt cgtccagctc
    14461 aagttcttct aatacagctt caggaacgaa atatacagtt aaagccggag actctgtttg
    14521 gagcgtctca aataaatacg gaattacgat ggctcagcta atccaatgga acaatatcca
    14581 aaataacttt atctacccag gacaacaact gactgtttca aacagtggat caacaaacag
    14641 cacgtcaggt aatacaggtt cgtcaaccaa tacaaataac agcagttcag ttaatactgg
    14701 cagcagttcg tctcaaggca cttataccgt gaaagcaggt gaatctgttt ggagcgtggc
    14761 aaataaaaac ggcatcacga tggatcagtt aatcgaatgg aataatatca aaaacaactt
    14821 catttatcca ggacagcaat tgatcgttaa aggtggaact tcggcaaata caaatacagg
    14881 ttcaactact tctgcagcga aaccaaatac accgaatacg tctgctacga caagtacatc
    14941 atccactggt aatacgatgt ataccgtgaa agcgggcgag tcagtttgga gcgtagcgaa
    15001 caaacatcat atcacgatgg atcagttaat cgaatggaat aatatcaaaa ataatttcat
    15061 ctatccagga cagcaagtga ttgtgaagaa aggttcatct caatcaacaa accaacaatc
    15121 agttactggt tcgaaaacct atactgtgaa atctggagaa tctgtttgga gtgttgctga
    15181 cagtcatgga attacaatgg ctcaactaat cgaatggaac aatattaaga acaactttat
    15241 ttatccaggt caaacgttga ttgtagctaa ataaaatagc tgttcgaaga aataacgatt
    15301 gaattccaca aaaatatttg ttactataag gctacttaaa tatgacaaca cttgaaaagg
    15361 aagtagtaaa aagactgaag tttcagagag cgcatggctg gtgagaatgt gcacggatgc
    15421 ttttgaactc gcctttgagt tgcccttgct gaatacccga gtaagcaagc ggcggtgacg
    15481 gccgttacaa cgttcgaggc cttttcacta aggtaaaatt aggtggtacc gcgtagatta
    15541 cgtcctataa aaagagcaat ctttttatag gattttttta tttcttttgt gaagtttgag
    15601 aaaacaaagc aaaaggaaat aaaaataaac agattctgcc ggttttttcg ttatatttat
    15661 aatagaaaca agtgttttca atagaacagg aggacagcaa atgagttaca atcataaaga
    15721 aattgagaaa aaatggcaaa aatactgggc aaaaacgaat actttcaaca cccatgatga
    15781 tcctgaaaag ccaaaatttt acgcactgga tatgtttccc tatccatccg gtcaagggct
    15841 acatgtaggg catcctgaag gttatacggc aacggatatc ctttcacgtg tgaaaagaag
    15901 tcaaggatac aacgtgctgc atccaatggg gtgggatgca ttcggtctgc ctgctgaaca
    15961 atatgcgcta gatacaggaa acgatccagc tgagtttacc caaaaaaata tcgaaacatt
    16021 ccgacgccaa atcaattctt taggatttag ttatgactgg aatcgcgaag tgaatacaac
    16081 tgatccggag tattataaat ggacacaatg gatctttacg aagctttatg aaaaaggtct
    16141 agcttatgaa gcagaagtag cggtaaactg ggtacctgaa ttaggaacag ttatttcaaa
    16201 tgaagaagtg atcgatggga aaagtgaacg tggtggctat gatgtagtca gaaaaccaat
    16261 gcgccaatgg atgctgaaaa tcactgctta tgctgatcgc ttattggatg atctagaatt
    16321 agttgattgg ccagaaagca ttaaagaaat gcaaagaaat tggatcggtc gttcagtagg
    16381 agcaaatatt gaatttaaag tggctggtac ggataaatcc tataccgtat ttacgacacg
    16441 accagatact ctattcggtg caacttactc ggttcttgca cctgaactag atcttgttag
    16501 agaaattacg acccctgaac aaaaagctgc tgtagaagct tatatcgaag agacagctaa
    16561 aaaatcagat ctaaagagaa cggatcttgc aaaagaaaaa acaggagtat ttactggtgc
    16621 ttatgcaatc aatccagtta atggcaagga aatcccaatc tggatcgccg attatgtatt
    16681 atcttcatac ggcacaggag ccatcatggc tgttccagca catgatgaac gtgactttga
    16741 attcgctcaa actttcggac tagaaattct tccagttatc gaaggtggag atgttgaaaa
    16801 agctgcttac acagaagatg gtacgcacat caactctgaa tttctagacg gcatgaacaa
    16861 acaagaagca atcgataaaa tgaatgaatg gctagaagaa aatggcgtag ggaaaaaaga
    16921 agtcagctat cgcttacgtg actggctatt ctcccgtcaa cgttactggg gtgaaccaat
    16981 cccaatcatc cattgggagg acggaacagt cacagctgtc ccagaagaag agctaccatt
    17041 acggttgcct aagacaaaaa acatcaagcc aagcggtact ggagaatctc cattagcaaa
    17101 tatcgaggag tgggtcaatg tcgttgatcc tgtaacaggt aaaaaaggcc gtcgcgaaac
    17161 gaatacaatg ccacaatggg caggaagctc atggtattac ctgcgttata tcgaccctca
    17221 taacaaaaaa gaactagcag attatgaaaa gttgaagcgt tggctaccag ttgacatcta
    17281 cattggtgga gcagaacatg cggtacttca tttactatat gcacgattct ggcataaatt
    17341 cttatacgat atcggtgttg ttcctacaaa agaaccattc caaaaactat acaatcaagg
    17401 aatgatctta ggcgaaaaca acgaaaaaat gtcaaaatct cgtggtaatg tagtcaatcc
    17461 tgatgatgtg gtcgaaactt acggtgcaga tacactgcgc atgtacgaaa tgttcatggg
    17521 gccattagat gcttcgattg cttggagcga aaatggctta gaaggaagcc gtaaattctt
    17581 ggatcgtgta tggcgtttga tcgttgatga gaacaataaa atgcgtgacc gtatcactac
    17641 attgaatgat ggcaagcttg ataaagtgta tcatcaaaca gtgaaaaaag tgacagaaga
    17701 ctatgaaaat cttcatttca atacagcaat ctcccaattg atggtcttta tcaatgaagc
    17761 ctataaagtg gacgctctgc cttatgaata tatcgaagga tttgttcaac tactcgcacc
    17821 gattgctcct cacatgggtg aagaactatg ggcaatcctt ggaaatgatg gtggtatttc
    17881 ttacgcacca tggccaacat acgatgaagc agcacttgtt gaagacgaag tagaagttgt
    17941 tttccaagta aacggtaaag ttcgtgcgaa aagcaatgtt ccccgagact taggaaaaga
    18001 tgagctagaa aaagcagcat tagccaatga aatagtgcaa gaatatattg aaggcaaaac
    18061 agtcagaaaa gttattgcgg taccaaacaa gttagtcaat atcgttgcta attaattggc
    18121 tctttgtcaa taaggactga tgagttgtgc aaaatcaaat ctgggccaga atgaacttca
    18181 ttctgtctca gattttttgt tatttcaaat tgattaattg attgatcaaa aaggttttga
    18241 ttttcatgtt ctcaaacacg ttcaatgtct ttttcttctg aatggtcttg taagagatct
    18301 ctgtaatcat caaatttttg tctattaatc ttagtatttt ttgatgtata aactacctga
    18361 aattgagagt acataccaaa ttcataaatt ttttattagt gggactagtg attgtatgga
    18421 ttttgacgca taatgaatga agtatgagaa aaggagagac aaatggatca agaactattt
    18481 attatcgaag tattatcagc taaacaaaaa attatcgaga aagcgaaaaa aagagcgaaa
    18541 agaataatgt tcaagctttt aagcttagta tttttctctg gtttgctttt tttcggagct
    18601 gtatcgttcc tatatcgcca tatgagtgca gtagtccgtt cagctgaagg gatgcaaaaa
    18661 ttaaataatt tgtatcactt ttcgtgggga gttggcggaa taacaattgt tgtgcttatt
    18721 gtttttcttc tacttatttg gctccgttac catcaattgt cgcgtcattt cgatcaatgg
    18781 cttagtcaaa gagcacaaca gtttttacat aataaaccaa cttctcaaga tgatgactat
    18841 ctttattttg agaagacttc gacgagtcaa gcttttcgga tacaaaaaag tgattgccag
    18901 ttattgctga gatcacttga tgatttacct atctacttag gcgaaagcaa atcaaaattc
    18961 ggaatgaaca cattccatat ttttttacta gaaaacgaac ccagatggga aaaaagccag
    19021 tcttcgcctg gtggatacct gacaactaag atgagtacat tagtcactgg ttttttcctc
    19081 attcttttag ctggaacaat ctattatggg agatacactc agccaaaaat gtcttctcat
    19141 ataaatgctg cttctcacat gacaatgaac tcaactaacg atacgcatat acaaaagaca
    19201 aatgaagggt tgttagctga tcctaagcaa aaggtaaatt tgacaacaca aaagataaat
    19261 gatctcaaac ttgatacaga gacgcactct ctctatatga caacagatag tggtgctaat
    19321 tgggtatttg tgccgattga ttcgcaatgg ttgcgttttg gcgaatacac cctgacaacg
    19381 gggctgattc ctgaaggtta ttggatggac aacacatatg atatttccgc cgatttctca
    19441 tggtttatct attcttctgg ggatgatgta cgtctgctga cttcaagaga taatggtaaa
    19501 acatggcaaa cgtctattat cacccaatca gttcagccga tacgttatcg aaaagcggta
    19561 ttttatggca acgatggtct atttatctgt acatcagcca gtgggatgtc tgcagaaact
    19621 ttgtacattt acactactat ggatggcgga atcacttgga taaaaagtgg aagtacaatg
    19681 atcaatcagc cagtacaaaa tgtcagcttc gttacaactt ctttaggttt tgtttcgaca
    19741 cgtgaaagtt tgttttatac aaacaacgga ggacgctcat tcaaagaatc agttctgtcg
    19801 attccggaaa actatcagac aggggggata gatctttttc aatcaccaaa tgaagttact
    19861 caaataagcg cgaatacgtt agagacaaaa ttttatttgt tgaaaaaagg accaatcgat
    19921 gcagggaaaa tgtttgcctg cctcttccgt tcaactgata acggggaaac atggcaattc
    19981 gttcagcaac tatcgcaagt agaacaagga gattaaagaa gagagattgt gagcctgacg
    20041 ttcagcaccg agtcacaaga aacaaattca aaaaacagct tctcatattt gttgaatagt
    20101 gtaactcgtg attcggagtt gacaagagca acaagaagcg attttgaagt atagcttctc
    20161 gtattttaca agatcgtgtc ttattgccga taggttgtct cttgaacacc gtttattcgt
    20221 aaaaagggat tgtgacaagt cttttgtcac tacttaccga acgaaactga aggagctatc
    20281 caaaaagtat cattagtatt attctttgtg tttctagtgg caccactagt acatttaaaa
    20341 aagctatcga tcatggacta atcatacatg atcgatagct ttttagataa tgtttgttac
    20401 gctatctgaa attgactatt ataaagactg gcataaagcg ttggtgattg gagtagttca
    20461 gaatgtgttc ctttttcgac gatatcgcca ttcttcatta caaggatcat gtctgcattt
    20521 tcaatcgttg acaaacggtg tgcaatcaca aaacttgtcc tatcttttgt cacagcttcc
    20581 attgcttgct gaatcaatgc ttctgttcgt gtatctacgc tagaagttgc ttcatctaaa
    20641 ataacgacag gaggattcgc tagaatgact cgagcaattg tcaataattg ttgctgtcct
    20701 tgagataacg tgccgttttc gctagaaatg atcgtatcat atccgtctgg cagtgttcga
    20761 ataaagtgat cacattgtgc gatttcagcc gcttcgataa tttgttctct tgtggcatcc
    20821 attttcccgt aggcgatatt atcagctacc gttccttcaa acagccaggt attttgtaaa
    20881 accatcccaa aagaacgacg tagttctcca cgtgaaagct tggtaatatc aagaccgtcg
    20941 aattcgatct ttcctttatt cagttcataa aaacgcatca gcagattgac taaagtcgtt
    21001 tttcctgcac cagttggacc aacgatcgct accgtttgtt ttggctttac tgaaaaatcg
    21061 acttgattca tcaatatttt atctgatgtg taaccaaact gtacattttt gaaagctatt
    21121 tttcctttcg gttgttctaa aagtcttgca tctggaaggt ctggaatttc atctgtttca
    21181 tctaatatct caaatatcct atcgattgct gccattgcgg attggatcga gttgatcaca
    21241 taagaagcgg tagaaattgg ttcagaaatc tgattgatgt actgaagata agcttgtaag
    21301 aagccgatcg tcacaccacc tgatagaaca agcatagcac cgaataccgc acttatgatg
    21361 aaggcgagtt gattaatgaa acgaatggcc ggatagatag cacagttcat aaattgagca
    21421 cgtttaaaag cgttgtattg ctttttattt acttcgtgta tagcagcttc tgcatgagct
    21481 tgctgattga atgttttgac cactagatta ccagctaaga tttcttccat tcgattattt
    21541 aaaacaccca gttcatactg attctgttca gaaatctgtt tgtttttgtt cgcaatctta
    21601 gttgtaacaa ttgaactgcc aacgatcagc aataagacga gcaaagtaag cttgctttcg
    21661 atataaaaca gcatcagacc agcagcagct attgttacga cagaagtaaa aaattgattg
    21721 atacctgtca ataacacttg agaaagttga ttgagaccgg tagttgtccg actaatgatg
    21781 tcacctactt gatgattgtc aaaaaacgac atcggtaatg ttttgaattt ttttgtgatt
    21841 tcttttcgca ccctcaatgt gatcttttcg cttaacgctg ccattgtccg ttcctgaata
    21901 aatgaagtga tgctgcttaa gactgaacag ccgatcagca ataagacagg gaacaacaat
    21961 gcttgtttga tatcactgaa agaaagctgt ccaatacctg tttttcttat ttctgataag
    22021 agatcatcga tcccgattcc catgatcaag ggcatggcta cgactaatag gttcccgaat
    22081 aaactgcata gaaacaatcc gtaaaaatat ttatgttcag gtttgatcat tttccaaaaa
    22141 cgtatcaaag cacgcgtgtt caacgttttt ttcttttcca tcgtatccct tctttctata
    22201 ttccttgtga ttttgcaaac tcctgataag tttggcaatt agacagtagt tcttcatgag
    22261 taccttgtcc aacgattttt ccttcatcta aaacaatgat gttatctgct tgttggattg
    22321 tactgaggcg ttgagcaaca atcacgagag ttttctccga catgtgttta tgaagggctg
    22381 ctcttaatgc tgcatctgtt ttatagtcta aagcggagaa actatcatcg aatacataga
    22441 tctctgctgg tttgatcaac gctcgagcaa tacatatccg ctgtttctgg ccccctgaat
    22501 aattagaacc gccttgagca acaaagctgt ccaatccgtc aggaagggtg tccactagtt
    22561 cttctaattg agcgatcttg atcgctttag acaactcttc tgttgaggca tattcatttc
    22621 ccatctgcaa gttgcttaaa attgtcccgc tgaataagaa agccttctga gggacataac
    22681 tgatgtggga gcggatctgt tcttgagtca tttgagtaag aggaatacca ccaagggtta
    22741 cttttcctat cgtagggtca ctaagtctca agagaagttt cgccaaacta ctttttccag
    22801 atccggtccc gccaacgatc gctgttgttt ttcctttagg tattgtgaaa tcaatatctt
    22861 ctagaactgg atcagagatt tcagtatatt taaatgtgac atgttcaaaa gcagctgcat
    22921 ttgtctgatc aaaggttggt tcagctactg ttttgatcgt ctcttcctca atttcgatct
    22981 ctgcttgcag tatttcttct atccgtcgta atgaggcaaa cgatttaggg ataatgacca
    23041 ataccatagc tgcgatcatc aagtaagcaa gtgtcagcat agagtattcg actacagcag
    23101 tcacgacacc gatttgtaat gctccttctg ctacaagatt tccgccaaac cacaaaatag
    23161 aagcataaac gattcccatg agcaaaaaag caattggagt gacccaagca aatattcggt
    23221 tcactttgat catttgatta gcatagtcag caaagctctt attcgttcgc ttttcttcat
    23281 atgattgatt atcaaaggca cggatcatat taatcccagt aaagaattct ctcaatgtga
    23341 tggtgatccg atccattttt ggctggatac tcaaagataa aggtgcccct cgtttcatca
    23401 gtcgataaac aatccataca taaaaaacaa cagagatcaa tgtaacccat gttagtaata
    23461 gagaataacg aaacatcatg agaatggtga aaacgcaaat aaccggtgct ggtaagatca
    23521 tttggcaaaa taaaacaatc atttgctgga cattatcgac atcattcgtc attcttgtca
    23581 aaagagatcc agaactaaaa cgactgatat ccttcattga cagctgttgg aatttacgga
    23641 aatatttctc ccgcgtttgg aatccaaacc tagcggaagc caatgctgaa aagtaactag
    23701 aggcaactgc agcgattgtt cctagaaacg ctacaagcag catttcaact ccaatttttt
    23761 ttataactga ggcttcgcca cttgctatac ctgtgtcgat cagctttgct actaattttg
    23821 gtacacctaa tgtaccggcg atttctaaac agatcatcac aaaggtaagg agagcgagtc
    23881 ctttgttttt ctgtaagaat gtttttaaga tagacatatt attcctcctt ttttcctgat
    23941 acttttcgat tctatacctt caagtaactt gaaggtcaat agaaaaaaga ataaactgaa
    24001 aaagaggtga gaaaaaatga aaaaaatgta tacgattggc caagtatcgg aactatttga
    24061 tttgccaaaa tcaacgatcc gctattggga tgaacaagga ttaatccgtt caagtagaca
    24121 agaagagaat gattatcgat tgtttgatat cgatgatatt tttatgcttt atgatatcgt
    24181 tttttataga aaattagata taccgatcaa acagatgaag aatttatatg gaaaaacatt
    24241 gacggagtta tatgagacac ttgatgaaac agaaagaaga atccataaag aactggtggt
    24301 gatgaaacaa aaacaaaaag agataaggga gcaaaaaaaa cagttgaaat tgatgatcga
    24361 tacaaacgag gaagaatttc ctgtagaaga gatccctttt gactgtatga tctcaacgga
    24421 gtttgaagat attgtggaaa tcaaaaaatt tctacctaac tattcaagct ttgggatgat
    24481 gagtatgcca gcttccagtt cacagacgat gtatggattt tttatcgacc ctagtgaagt
    24541 acatttattt actcaagacg tgatttggga gaaaaaagat acggctgttt atcgtcgatt
    24601 cttattgaaa tccgagatga accatgcgga acgtaataat attttagaaa tcagagaaag
    24661 aatgtatgaa aaaggatgga aaacaggaga agtgatcggc cagtatctat tgacgaatac
    24721 ggatgaaaac aatatcagaa ctgaatatta tcatgcatgg atagaaatga aaaaatagga
    24781 aatatgtgtt taagataact cgaaaatgat ggttgtttta gttataaaga aaaattttct
    24841 ggttcgatta cgggtttaat gtgggggatt attagtcgtg gatagataga attttagttt
    24901 tttagaggaa atatgtcttt aaaaatggaa tggttgcaat tcttagcgat atatacgatg
    24961 taattgtcct cttaaaaaag tatggaaaaa taattgtatt gggtgttatg tataattcat
    25021 ggtgaactga gactaatgag aagaaaaatt catattgtta tgagtgagat tgtatcagat
    25081 gagaatgata aattaaatag gtgcataaaa gcattacact taaaatacat tgttgcttac
    25141 attattacaa tatgttaata aggcaaaaat aaatagatgg attgaataaa catatttttt
    25201 aatcaaatag tccctactgt gactttcctc agctatcggt gatactaccg atagcatttt
    25261 tttgcttata aaataattat aagtatttag agatttttct tctaacattc ttcaaacaag
    25321 aaaaatcaca aaggttattg ttatttttat atattctttt taagaatgat tgttttttta
    25381 tagaaatttt aaaaaataaa acacgttatt gatggatagt tagtttgtta tttggtaata
    25441 ttatagaagt tgactggtag tgatttattt ttcgtcgttt tataaaagtg ttattttttg
    25501 gaaaaattca caaatatgta acgaatgttt ttctggaatt tgctgtgtta aaaagcagta
    25561 tgactaatat aaatactatt gtcatttatg caatttttta tggatagtaa ttttagaggg
    25621 gaatcaatga aggaagaata gaagggaaaa atagttgcaa atacgccagt tagcaaaaat
    25681 agcaatacat cttcaagctt taaaattcga gagaatcatc ttggtagtgg aatcaactac
    25741 aacaaaagga ttaaaccaat taatagatag aattaccaat ggaaaaatag aaaaggtagt
    25801 tattctatac aaaagttgtt taattcgctt cggttatgaa cttagtgaaa ccctttgcaa
    25861 tatatggtac agaatataga aattattgac attacagaaa atgatgaaga tttaatacag
    25921 attgtcacta tttgtagata ccttttacaa ggaaaccgag ccaataaagc taagaagatg
    25981 attaaggagt tgatggaaga cgatacaagt taagaaagtg aaactacgcc caaataaaga
    26041 gcaaaaagag taattggacg tggaaaatgc aagaaatgaa ccatcgattc ggtggacagt
    26101 ttattagtaa tgacggtttg agaacatcca tgacgagaat gaaggaacat cctaaatgcg
    26161 tttagttgaa ccaagtgcca aataatatgg ccaaataagc gatcagttat cgatgaatcc
    26221 caagtattct aaactatata ttcattttga tgctaagcat tgatatattt ttttgggatt
    26281 gatgaaggtt aaccaataca gggattcact gatgaaattc tagagtctga ccttggtatc
    26341 aacgatttag tgattgtttc taatggaaaa gtataaaagt atataagtac attaacaaaa
    26401 taactattag ggttacgctc cagcttgtgc aaggcactgg caggaggaat cgtgaaatca
    26461 taattatggt taagccacac ggacaactaa aagtcataca aagctatcga atcaatcaac
    26521 agaagacaat cggcaatcat tagctttgtt tacagcttac attgcaacgc ttgagttgaa
    26581 ccgaaaatac gagggaggta acgcagccat tttgagttgg ggaatctttt tagaaaatta
    26641 tcgggtacct tgttgtttag tcaaaattat aagggggagt aatagtgagg aacaaatttc
    26701 ataaactgtt aagtatgttt ctagttatgc ttattgtgat caatcaattt gggaacataa
    26761 cggttctagc tgagtcttta gcagaacagc cagagcgttc gctgacgatc gaagttgatc
    26821 aagaggaggc tgctttgggt gagcagttcc agttgcgagt cagaggaaca ccagacctgc
    26881 taaatgcatt ggtcattgat aagagtgagg atttgattga aggggaagtg aagcaactca
    26941 atgattacga acgtctgatt acttattctt cccagcattc aggtaaatac gaattgaccg
    27001 caagaagcgg tgatattcaa tcaaatacgg tctcaataga aatcagtgta ccagaagaaa
    27061 gcacaagtga gtcttttaat gtaccagaag aaagcacaag tgaatctttt aatataccag
    27121 aagaaagctt aagtgaatct tttaatgaag ccagatcatc tgagccatct ataacgcagg
    27181 aatctcagat tgaaagacaa gaggagctct tgaattatga catcggcatc tctgctgagc
    27241 gccccacttt gccgagcagg catacacaaa cgttaaaggg atacattcaa ggaggcgctg
    27301 gcgatattct tgaagaatat ggggagatgg tcgtacgagt tcctagagca tcgattggct
    27361 taattgagtt cattagagtg gaggtcgctg atgattcacc tttaaagcca ctggcatttt
    27421 accaagaagg cacagacgtt gtttttccat ttacctttga ttatgaagga ttaaatgaag
    27481 ccgctcagtc gtatacattc agtatcacgt ttgaagttgc tttctttcaa tcaggcggta
    27541 ctgagccaaa taatgacttt caagtgagtc ttacttatgc tgagcgagca gcaaacacgg
    27601 aattttcaat tacgcctatt ccttcatcaa gacccatttt ttcgaaatta tactatggct
    27661 ctagagaaat cgacagccaa ggaaatcttg ggaatctgct gattgacggg caagacgtga
    27721 ccaacagtaa taaatttatt ttgcaggtaa attatgaagg atcacgaaaa gacggtgtag
    27781 tggttaccga tgtactaccg caaggaacaa ctttggcacc gaatccgact acatggagtt
    27841 ccactggaaa tagagaacct atttcaggtg gggttcggat cgcagaagta acgaatccag
    27901 gtgaagcaaa cgttagaact cattatgttt ccgaggaatt tgtggacaaa gtatctttta
    27961 ataaggaaac gaatacactt gttgtggact ttggtaatct agcagagggt caatcgtatt
    28021 ttattgaata tgctcttgaa gtgaccgatg tcgacttgtt caatgaagga agacaagagg
    28081 atgggttttc tcggaatcga gcgagtatga cctacaataa tcaggtaatg agccgagact
    28141 accgcttgag aatccctgaa agtaatcttc cggcaatctc tttaagaaaa agtgtggaca
    28201 aaaatgttat caatctcaat gaaaatgaac taacgtatac tctgcatgtc tcgatgtttt
    28261 ctggaacaag aaatacgctt gttattgaag atgttttgga agaaggcatg gaatttgtgt
    28321 catttctatc agatattccc tctttcattg atgccgaagt caataatgga atagttcgtt
    28381 tcagtgcgaa taatacgatt ggtggtccga caagttttac cctctctttt aaggtcaatg
    28441 tagaggcgta tgcggtcggc gatcaaattt taaactttgc aacgcttgtg aatagtgaca
    28501 atcgattgaa tacgagtact gtaagaacaa ggaaaatcga tgggcgtatt aaagtaatta
    28561 aaactgatga gaatggccaa aggctggcag gagcaaaatt tgaaatttta tcaagcgatt
    28621 cacaagtgat acaagaaggt ataacggcag aaaacggaga atttttaagt gaaccgcttc
    28681 ctgtaggtag ctatcaagtc cgagaaacag aagcaccgga agggtatctt ttagatgcga
    28741 cggtacatga tgtttttata aatgaatact ccatagaaga agtgacgtta accattgaaa
    28801 acatccttga tacgggtggt gttgaactaa ttaaacatgc tgaaggcagt ggtgaagtcc
    28861 t
//
LOCUS       DARBET010000029        28008 bp    DNA     linear   BCT 07-NOV-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4027
            SAMEA114569841-rid20112183.denovo.029, whole genome shotgun
            sequence.
ACCESSION   DARBET010000029 DARBET010000000
VERSION     DARBET010000029.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA114569841
            Sequence Read Archive: ERR12203666
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 28008)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 28008)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001972618.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001972618.1
            Genome Coverage       :: 132x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/07/2023 13:18:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,041
            CDSs (total)                      :: 2,975
            Genes (coding)                    :: 2,908
            CDSs (with protein)               :: 2,908
            Genes (RNA)                       :: 66
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 67
            CDSs (without protein)            :: 67
            Pseudo Genes (ambiguous residues) :: 0 of 67
            Pseudo Genes (frameshifted)       :: 26 of 67
            Pseudo Genes (incomplete)         :: 28 of 67
            Pseudo Genes (internal stop)      :: 20 of 67
            Pseudo Genes (multiple problems)  :: 6 of 67
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..28008
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA114569841-rid20112183.denovo.029"
                     /isolate="VRE4027"
                     /db_xref="taxon:1352"
                     /country="Denmark"
     gene            8..649
                     /locus_tag="SCJ86_001433"
     CDS             8..649
                     /locus_tag="SCJ86_001433"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005879904.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEG1168978.1"
                     /translation="MIERSTFLYVQLQENKTLLHFSPEFHLEGLTLGEIYQAEGLSAV
                     LHFFKVELELPVKDYIELSLESWDRAVVELFPEGLMIDTNDGKIHLTAAELQKQMRYQ
                     IDDENSFSIFRRQQKILKSFLKVISKKRNLIKTTQLLKKYPGLLHTSIQFPQLITMIH
                     RFIRMQQLPSKKLFLPLTDTFRVVNREDKKKVIVIDFTRNRNVLHQVAMEKAN"
     gene            708..1148
                     /locus_tag="SCJ86_001434"
     CDS             708..1148
                     /locus_tag="SCJ86_001434"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEG1168979.1"
                     /translation="MMQTWLERLIRFQQNYHKLMQGRYAKFDQLNRGLLIAALVFSLL
                     SRWLPYRIGQLLFIVFFGWMIFRFLSKKIYPRLNENQRYLKYLDRVKEKYHQIKNKKQ
                     VKMDRKSYTFFECPNCHQKQRAPKGKGRIRVTCKTCGIKFETNV"
     gene            1160..1828
                     /locus_tag="SCJ86_001435"
     CDS             1160..1828
                     /locus_tag="SCJ86_001435"
                     /EC_number="5.4.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326957.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2,3-bisphosphoglycerate-dependent
                     phosphoglycerate mutase"
                     /protein_id="HEG1168980.1"
                     /translation="MKLVLLRHGESEANFENYWTGWLDVALTEKGKEQAAVAGQKMLE
                     AGLQFDAVYTSVLKRAILTGQVALEEMGQLWLPIIKTWRLNERHYGDLVGKNKDKMKE
                     IYGKDQVKTWRRGYTEVPPLTAENRFDRRYDHLDPHLIPYGESLEMTVNRIIPLWQDH
                     LAPKLKDRQDLLVVGHGNSIRALTKYLEDVPEDQMDTIDIPNAQPIQYTLADDLTILD
                     KKIL"
     gene            1892..2515
                     /locus_tag="SCJ86_001436"
     CDS             1892..2515
                     /locus_tag="SCJ86_001436"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD-IA family hydrolase"
                     /protein_id="HEG1168981.1"
                     /translation="MKNYIWDFDGTLFDTYPAMVDGAWQALKDFGISMDKKEIYFKMK
                     KYSTSYLINESNLNAREFNELFHRYEKESTEVSRPFPETKQVLEMLKDNGGRHFILTH
                     RLTESTWGLLKEHRLAHLIEEVVGIDQDFPRKPDPASLNYLIDTFHLERTDTMMIGDR
                     RLDIEAGKNAGVATCLYDIDHFLGEIPADYVVGNLNEILTLQSIIKK"
     gene            2571..2957
                     /locus_tag="SCJ86_001437"
     CDS             2571..2957
                     /locus_tag="SCJ86_001437"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327858.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1149 family protein"
                     /protein_id="HEG1168982.1"
                     /translation="MEIRRQKEVVEAFHYDMRLPNQEVETDLKVGFSPLKETIENYPK
                     EHSIVGARLEFRLVFDTYVLSGSVSQINHILNRKIEGQNDVTQEEVDELVAPLFDIVR
                     RMSYEVTEIALDQPGVKLNFQSEKQE"
     gene            3170..4561
                     /locus_tag="SCJ86_001438"
     CDS             3170..4561
                     /locus_tag="SCJ86_001438"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005879894.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-cystine transporter"
                     /protein_id="HEG1168983.1"
                     /translation="MTTLITVLVVLVFAALLYVFYRMQKRHTKFSTRVFTALGVGIVF
                     GGLLQLIFGVDDKVVTQSMEWIGIVGQGYVSFLQMLVMPLVFISIIGAFTKMKVSEKL
                     GKISATVLTSLLGTTAIAALIGIGSTMLFGLQGAKFTQGAAETARISELATRQETVEN
                     LSIPQQILSFIPKNVFADLAGSRATSTIGVVIFAAFVGIAYLGVKRKAPKEAAFFSDL
                     VDSLYKIIMRIVTLVLRLTPYGVFALMTNVLATSDFAAIVNLGKFVVASYVALLAVLI
                     VHSLILIGAKVNPINYFKKAFPVLSFAFTSRSSAGALPLNIETQTKALGVDDATANFS
                     ASFGLSIGQNGCAGVYPAMLATIVAPAAGVNVFSLEFILMLVAIVTISSFGVAGVGGG
                     ATFASLIVLGAMNLPVAIVGLVISVEPLIDMARTAVNVSGSMVAGIVTSARIKDLDRD
                     VINDDSKVIDVNA"
     gene            5172..5540
                     /locus_tag="SCJ86_001439"
     CDS             5172..5540
                     /locus_tag="SCJ86_001439"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329268.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lactoylglutathione lyase"
                     /protein_id="HEG1168984.1"
                     /translation="MFTNEIKIMLYVDDVEKNATFWRAVGFAEKDRQEMDGTLIIEVG
                     VEDSQTSFLLYDRAFVESHSPGTATSAPSLMFYSKDIFGLYKKMQTQSVTLGDMVQLG
                     EEYVFNFADPDGNYFAVTGK"
     gene            5720..6661
                     /locus_tag="SCJ86_001440"
     CDS             5720..6661
                     /locus_tag="SCJ86_001440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331409.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhodanese-related sulfurtransferase"
                     /protein_id="HEG1168985.1"
                     /translation="MDYQVLLYYNYTKIEEPEEFAKNHLAFCKSLDLKGRILVAEEGI
                     NGTVSGTVENTTAYMKAMKADPRFADTMFKIDPSEGHAFKKMFVRYRPELVSLKLEED
                     IDPNQVTGAYLSPQEFKEAILDEQTVVIDARNDYEYDLGHFRGAVRPDIRSFRELPDW
                     IREHKEEFMEKRVVTYCTGGIRCEKFSGWLVREGFKDVGQLHGGIATYGKDPEVQGEL
                     WDGKMYVFDERIAVDINQVDKQIVGRDWFDGTPCERYINCSNPECNRQILASIENEEK
                     HLGACSIECAKHPRNRYVQDHGISPEEAELRFTALIK"
     gene            6730..7461
                     /locus_tag="SCJ86_001441"
     CDS             6730..7461
                     /locus_tag="SCJ86_001441"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325746.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine/threonine protein phosphatase"
                     /protein_id="HEG1168986.1"
                     /translation="MKSLIYAIGDVHGKHELFEKLIHYYDPKIHQLVLLGDLNDRGPR
                     TKECFLLGKQLVEETGAIYLRGNHEEYFLQFLQSPEDWYAPYMRNGGKETFESLIHPG
                     ASEEYSPTEISMMIQSRYSELIKFLVQRPLYYEWENYLFVHAGVDLTKKDWRKTSLRD
                     FIWIREPFHEGKNNTGKTIVFGHTITPMLHGDMQTTDLWIQDHKIGMDGGAVFGGSLH
                     GVVFDKEGILQDIEYQNNEGPWHGI"
     gene            7605..9785
                     /locus_tag="SCJ86_001442"
     CDS             7605..9785
                     /locus_tag="SCJ86_001442"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306554.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-binding transcriptional accessory protein"
                     /protein_id="HEG1168987.1"
                     /translation="MTENLNQTIIQLVQKELSDYRPKQLTTVLNLLNEGNTVPFIARY
                     RKEMTGSLDEVQIREIEERYAYLENLEKRKTEVIRLIDEQGKLTPELEAEITQAVKMQ
                     QVEDLYRPYKQKRRTKATIAKEKGLEPLAKWLMQLTDGEVQSEAEKYIDKEKEVSSAE
                     EALHGAHEIIAEQVSDNAKFRTWIRSYTYNKGMYVSNVKDEQADEKGVYEMYYDFAEP
                     VHKMVSHRILATNRGEKEDILKVFFQVDEAAILAYLDRQIVKNPASPSSSFVREAYQD
                     SYKRFIQPAIERELRNELTEKADEQAIAIFGENLRNLLLQPPLKGKVVLGFDPAYRTG
                     CKLAVVDATGKVLAIEVIYPHKPAAQAKREAAGPAFIQLINKYQVDMVAIGNGTASRE
                     SELFVAEQLKSADHKTYYAIVNEAGASVYSASEIARKEFPHLQVEERSAVSIARRLQD
                     PLAELVKIDPKAVGVGQYQHDVSQKRLAEQLDFVVETAVNQVGVDVNTASPQLLQHIS
                     GLNKTTAQNIVIYREENGEFTARTQLKKVPRLGPKAYEQAIGFLRVPGGKNILDNTGI
                     HPESYSIAKEILTSVQLSEKELGTEEAVEKLTRLSVEKLAESLSVGEETLADILAGLT
                     QPGRDMRDEMPAPLLRTDVLSMEDLKPGMELTGTVRNVIDFGAFVDIGVKQDGLVHIS
                     KLSKKFVKHPTDVVSVGDIVTVWIEQVDTKKGRISLTMLSPYEE"
     gene            9805..10287
                     /locus_tag="SCJ86_001443"
     CDS             9805..10287
                     /locus_tag="SCJ86_001443"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331406.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SprT family protein"
                     /protein_id="HEG1168988.1"
                     /translation="MTQEELQQLVEEISLKSFQLPFKHQAMFNPRLRTTGGRYHLNDH
                     HLDFNAKLFEESDEETIAGIIKHELCHYHLHLAGRGYQHKDMDFKYLLKKTGGLRYAP
                     ALTKKQIKMEYYRCQDCSATICRKRRIDVRKYRCGRCHGEIKWIETKREDFNEAQKIR
                     "
     gene            10303..11064
                     /locus_tag="SCJ86_001444"
     CDS             10303..11064
                     /locus_tag="SCJ86_001444"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296401.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdcF family protein"
                     /protein_id="HEG1168989.1"
                     /translation="MEKSEQRNLKDWNQLLNFLAAPEEGNFEELTAPTLILAGNCLPI
                     LADKAAQMYLNGQVDQLFLVGGVGHATRILYENFEKQGFHFEDGMSESEICRQYLKEV
                     YDLPDKAFLIESKSTNSGENATFSLEILHSLGAVPEKILLMNDPTLQRRTRATFEKVW
                     QNEQTIFANAVPFVPEILHFSEEIIFTAKELNHQWPKEYFHALVLGEMERLHDDENGY
                     GPKGKDFIPHIDISEEVWSSYTRIKESIKTDFSRT"
     gene            11195..12529
                     /locus_tag="SCJ86_001445"
     CDS             11195..12529
                     /locus_tag="SCJ86_001445"
                     /EC_number="6.3.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016624780.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramate--L-alanine ligase"
                     /protein_id="HEG1168990.1"
                     /translation="MENQNKLYHFVGIKGSGMSSLALVLHEQGLNVQGSDIEKYFFTQ
                     RDLEKANITILPFNADNVKPGMTIIAGNAFPDSHEEIQRAKELGLEVIRYHDFIGHFI
                     QNYTSIAVTGSHGKTSTTGLLSHVLSGVRPTSYLIGDGTGHGDPQAEFFSFEACEYRR
                     HFLAYSPDYAIMTNIDFDHPDYYTSIDDVFTAFQTMAGQVKKAIFAYGDDAYLRKLKA
                     NVPIYYYGVTENDDIQARNIERTTSGSAFDVYHGDEFVGHFTVPAFGKHNILNALGVI
                     AVAYFEKLDLKEVAEEMLTFPGVKRRFSEKIVADMTVVDDYAHHPAEIKATIDGARQK
                     YPDKEIIAVFQPHTFTRTIALMDEFAEALDLADKVYLCDIFGSAREEQGNVKIEDLGA
                     KIKKGGEVIKENNVSPLLDYHDAVVIFMGAGDVQKFEQAYEKLLSSTTKNVL"
     gene            12836..13321
                     /locus_tag="SCJ86_001446"
     CDS             12836..13321
                     /locus_tag="SCJ86_001446"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dehydratase"
                     /protein_id="HEG1168991.1"
                     /translation="MNIGKPRKLGKTINEIEEGDSLSLTESIEDNQLLLYLGLTNDAN
                     PLYIQHDFAQKTEYQQPIVPSVMLMGIITSAISKHLPGPGSHVVNFSVNFIEPVFHYE
                     TLTFQFEVIKVDKMKDVVTISVEAENTNEDRVLDAVVMVQPPQVPELEIAIESEGELN
                     E"
     gene            13314..13997
                     /locus_tag="SCJ86_001447"
     CDS             13314..13997
                     /locus_tag="SCJ86_001447"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300670.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Bax inhibitor-1/YccA family protein"
                     /protein_id="HEG1168992.1"
                     /translation="MNNPSTSMSGINRFYAKVYGFLGMGLALSAVTSYLALWVFPLQL
                     ATFINNFPLGFVGLWIVELILVMVLGVKAQKNPSLAIGGFIAYSLLNGIILAVTLAYY
                     DIGTVTQAFLTATLMFMGMSVVGVTTKRDLSGVGRAGQAALIGVIIALVLNIFILRSS
                     VVELFISLLLVVIFAGITAYDNQSIRNIYVQTNGTAHSGIAVFMALKLYLDFINLFLA
                     FLRIFGRNN"
     gene            14123..16768
                     /gene="polA"
                     /locus_tag="SCJ86_001448"
     CDS             14123..16768
                     /gene="polA"
                     /locus_tag="SCJ86_001448"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345146.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase I"
                     /protein_id="HEG1168993.1"
                     /translation="MTKNKLLLVDGNSVAFRAFFALHNSLERFKNRNGLHTNAIYAFN
                     TMFENVMQKEQPTHVLVAFDAGKTTFRTEMYAEYKGGRSKTPGEFKEQMPYIRDLLTG
                     LGVQYYELPNYEADDIIGTLAEKVDKDQFDVVVLSGDRDLTQLASKEVKVDITVKGVS
                     DIESYTPEHVAEKYDGLTPKQIIDMKGLAGDASDNIPGVTKIGEKTAIKLLKQYGSVE
                     GIYEHIDEMKQSKMKENLINDKEQAFLSKKLATINTSAPVDVSIDSLKYEGKNLDKLV
                     PFYKEMDFNQFLSKLNIVEEEVKMDDILFEVVHEFKEEMFTTDMALYVEMMGDNYHTE
                     EIVGVAWGTEKKIYVTNDLSVFENRAFHSWITDPTRLKKVYDAKRTYVALNRYTGKTK
                     GIDFDVLLGAYLLDTNEKSTDIEGIAAHYGYNDIQSDEAVYGKGAKKGLPEEEELFFA
                     HLARKVAAINALTPKLSQELAEKNQEDLFRKMELPLSIILAEMEISGIKVDATRLQEM
                     KGEFSARLREIEQKIYEEAGEEFNLNSPKQLGVILFEKMGLPVIKKTKTGYSTAVDVL
                     EQLREQAPIVEDILTYRQIAKIQSTYVEGLLKVIQSDGKVHTRYVQTLTQTGRLSSVD
                     PNLQNIPIRLDEGRKIRQAFVPREKDWLIYSSDYSQIELRVLAHISNDEHLKAAFLEG
                     QDIHASTAMRVFGIEKAEDVTPNMRRQAKAVNFGIVYGISDYGLSQNLGISRKAAQQY
                     IDTYFEKYPGVKEYMERIVREAKDQGYVETLYHRRRYLPDINSRNYNIRSFAERTAIN
                     TPIQGSAADILKIAMIELDKRLKETGLQATMLLQVHDELVFEVPEKELESLDKLVKEV
                     MEQAVSLHVPLITDSSWGKTWYEAK"
     gene            16813..17649
                     /gene="mutM"
                     /locus_tag="SCJ86_001449"
     CDS             16813..17649
                     /gene="mutM"
                     /locus_tag="SCJ86_001449"
                     /EC_number="3.2.2.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002390879.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-formamidopyrimidine glycosylase"
                     /protein_id="HEG1168994.1"
                     /translation="MPELPEVETVRKGLERLVVGKTIQKVQVLWPKIIEQPETPIFEA
                     SLVGETIQSIGRRGKFLIFHLDHCELISHLRMEGKYQFTKENTPIDKHTHVLFFFEDG
                     SQLRYNDVRKFGRMTIVEKGASATYRGIMKLGPEPLPDSFLLADFANGLKKSHKAIKP
                     LLLDQRLVTGLGNIYVDEALWEAKIHPEQPANTLRPKEVEQLRLSIIDVLDRAIEAGG
                     TTIRSYLNALGESGGFQVALHVYQQTGKPCIRCGTPIVKTKVAQRGTHYCPNCQRLKI
                     AK"
     gene            17613..18242
                     /locus_tag="SCJ86_001450"
     CDS             17613..18242
                     /locus_tag="SCJ86_001450"
                     /EC_number="2.7.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340744.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dephospho-CoA kinase"
                     /protein_id="HEG1168995.1"
                     /translation="MSELSKAKNRKVGFVLGLTGGIATGKSTAAKVFQSHGFPLIDGD
                     VIARETVEPGTLALRKIVSVFGQEVLQPDGQLDRGKLGMIVFPSKEKRQKLDQLLDPF
                     IRKAIKDQIASLSSNHPLVIVDIPLLYEGHYDHYMDAVAVVYTTPETQLQRLMKRNQL
                     TLEQAQQRISSQLPIEEKKQRADILFDNNGTKEILVGQIENWLFANRFL"
     gene            18294..18626
                     /locus_tag="SCJ86_001451"
     CDS             18294..18626
                     /locus_tag="SCJ86_001451"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CsbD family protein"
                     /protein_id="HEG1168996.1"
                     /translation="MGSLKNQKDKVVGNAKEKIGEMLNKENLIDEGRTQSETAEEKEK
                     QYQARIDEIEDQQAEEEAKRQDLTQERKNPTPDRSKTPLPESDHIPDEQKIEEQRMKH
                     YTGIEEKL"
     gene            18938..19429
                     /gene="nrdR"
                     /locus_tag="SCJ86_001452"
     CDS             18938..19429
                     /gene="nrdR"
                     /locus_tag="SCJ86_001452"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288327.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional repressor NrdR"
                     /protein_id="HEG1168997.1"
                     /translation="MRCPRCQHNNSRVIDSRQADDGRAIRRRRECENCSFRFTTFERI
                     EAAPLLVIKKNGEREEFNRDKILRGLIRSAEKRPVAMEQMEQIVNHVENRVRSLGENE
                     VSTNLIGEYVMEDLVDLDEIAYIRFASVYRQFKDMSVFLKELQDIVDKAKENNKENNK
                     ENN"
     gene            19452..20825
                     /locus_tag="SCJ86_001453"
     CDS             19452..20825
                     /locus_tag="SCJ86_001453"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324939.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DnaD domain protein"
                     /protein_id="HEG1168998.1"
                     /translation="MENAWKELQPKNIYQGRKAAPLREDSKNALLYLYQPLIGGEALS
                     IYFTLLSEISQESGKGPEGMHADLLSSLGCGIPQFYDARKKLEGIGLLDVYFKEDSDL
                     GPRFIYELLEPLIPKAFFKDSVLAFLLLEKVGERRFQKLTQFFEPKRHTAEGYQKITK
                     KFLDVYRFNEAVYAANQENIESLNKSFEESESVALLEADSTFDWQFLNDWLAKKHIAS
                     LDDPIIKQVDMYHQLYGFDELTLGELISQSYDFSEQKVSEKELQKAILAMQRRPVKQT
                     KETVAVSENSNEPSSNEHLTNAALQLIKEAQSIPPMKYLEAIKSEKGGYVSKQENWLL
                     QDLVSQSGLSSSVINVLLNYVLVIKNNASLNASYVNTVANEWAQKKIATAEEAIQHIR
                     QISNQAKQSKQKKQTNYQTGKRNVRREKLPDWVNQPKDEKQISSEKKAEIDRRFKEYL
                     AKKEGDI"
     gene            20825..21751
                     /gene="dnaI"
                     /locus_tag="SCJ86_001454"
     CDS             20825..21751
                     /gene="dnaI"
                     /locus_tag="SCJ86_001454"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="primosomal protein DnaI"
                     /protein_id="HEG1168999.1"
                     /translation="MEDVGKELKRIISKRNYQEKYAEMIAEVLNDADVQAFLSAHQEE
                     LTQADIEKSYAKLYEFVQEKRKYQKNDPTMIAPGYEPKLTLNFHYVDVTYVPTEALLA
                     RQRQEEIRSRVKAMDMPKDIQSASFENFERTDGRGYASLEALDFVEKYTADPKGYHKG
                     LYLVGSFGIGKTYLLGATARELAIAGFTTTLVHFPTFAVEMKQAIGKDQVAEKLDAVK
                     RSPILMIDDIGADAMSSWIRDEVFGVILQYRMQEQLPTFFSSNFTMDELEQHLSVTQR
                     GDEEPLKAKRIMERIRYLTKEIEMTGRNRRNG"
     gene            21880..22320
                     /locus_tag="SCJ86_001455"
     CDS             21880..22320
                     /locus_tag="SCJ86_001455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein"
                     /protein_id="HEG1169000.1"
                     /translation="MRAIFSSISVVGLFFLLAGCGSADSNTGSVDSIDAGQTVKTSST
                     KTERQSIFVGTIESITQVEAEEETLQLKLVDVMAKEDPANMGTSFSNDGVILNATAEK
                     ISVDINELKIGDKVQFTLVEMPIMTMSIPPQVPGDSIIDVSLNK"
     gene            22464..23165
                     /locus_tag="SCJ86_001456"
     CDS             22464..23165
                     /locus_tag="SCJ86_001456"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288335.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (adenine-N(1))-methyltransferase"
                     /protein_id="HEG1169001.1"
                     /translation="MNHLDLSQRLAAVGDFVPESARLADIGSDHAYLPVALILRGKIE
                     YAVAGEVVQGPYESARKQVKKDGLEDRITVRLANGLDAIQPQDGITAVTIAGMGGSLI
                     RDILEAGKLNQRLSGQERLILQPNIGERTLRSWLQENQYKIQSEKILEENKKIYEIIV
                     AEKEQKPVTYNEMELMFGPFLLEEKSEIFKSKWTRELQQRKAIREQLKQAANQEARQK
                     QIEQEIQMIEEVLKP"
     gene            23162..24283
                     /locus_tag="SCJ86_001457"
     CDS             23162..24283
                     /locus_tag="SCJ86_001457"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309063.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Nif3-like dinuclear metal center hexameric
                     protein"
                     /protein_id="HEG1169002.1"
                     /translation="MTISGRTFIQKFESYCPQWLAEEGDPVGLHIGTLDKPIQRVMMT
                     LDVRPEVVEEAIKKKIDLLIAKHPPIFRPVKRLVTDQPQEKMYADLLKHDIAVYAAHT
                     NMDIIWDGLNDWFCELLGIEVESYLVKTHEIHYKKLAVYVPVDHAQKMREVLAAAGAG
                     TQGDYTGTSFTSIGHGRFTPEAGAQPAIGKVGKTEQVQEAKVEVILPETIEKQVIQAM
                     RSAHPYEEPAYDLFAIDEPVEMFGLGRVGELPQEISIEAFVEQVKEAFQLDGLRIVQP
                     KNAKSSVKRIAICGGSGEKFYPQAIAQRADVYITGDIYYHTAHDMQSAGLIAIDPGHY
                     IESLCKQRFIEKFESWKQEENWDINFFVSETNTNPFQFN"
     gene            24298..25530
                     /gene="pepT"
                     /locus_tag="SCJ86_001458"
     CDS             24298..25530
                     /gene="pepT"
                     /locus_tag="SCJ86_001458"
                     /EC_number="3.4.11.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008267310.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase T"
                     /protein_id="HEG1169003.1"
                     /translation="MYENLLPRFLRYVKTETRSDATSKTTPSTQTQVAFAQELKKELE
                     ELGLADVFYNEDNGFVMGTLPSNVDHEVRSIGFISHMDTADFNAVGVNPQIIENYDGE
                     STIVLDAEERYTLNTKDFPNLKNYEGHTLITTDGSTLLGADDKSGIAEIMTAMEILIN
                     NPSIPHGTIKVAFGPDEEIGVGADKFDVEQFDVDFAYTVDGGPLGELQYETFSAAQAN
                     ITIQGKNVHPGTAKDTMINALQLAIDFHNQLPADEVPEKTEGYEGFFHLMALDGNPEE
                     ASMSYIIRDHDREKFEARKAQITHIQEELNQRFDQERIKVDMYDQYYNMKEIIEKDMS
                     IVELAKQAMIELGISPVIEPVRGGTDGSKISYMGIPTPNLFAGGENMHGRFEFVSLQV
                     MEKATDVIVKIAELNTKS"
     gene            25662..26570
                     /locus_tag="SCJ86_001459"
     CDS             25662..26570
                     /locus_tag="SCJ86_001459"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002391050.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YegS/Rv2252/BmrU family lipid kinase"
                     /protein_id="HEG1169004.1"
                     /translation="MEKLLVFYNKSSGKDEGEKLARWFQDYVKEKQPELYVELAETGP
                     SIDDAELVKKAEAIEADTLVIIGGDGTIHHIVQAFQKTLSQYHVGLLPGGTVNNLARV
                     LEIPLKKEEAADVILEGNVRKIDYGQVNDEVIISTLTIGILADTAVWISQEEKQRYGS
                     WIFIKKFFKLLLKKKKYHLDLETDEEHWKGKSQLLTVTMSNSVGGFTNFDDSATLDDG
                     KFHVTIVPSLDIFRFIFYLPRIMRGKFYTIPGITYFTAKRMKIQAQEKSVGTRTDGDT
                     TDDLPVELSVVSKGLTVYSPKKEEKA"
     gene            26605..26877
                     /locus_tag="SCJ86_001460"
     CDS             26605..26877
                     /locus_tag="SCJ86_001460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1294 domain-containing protein"
                     /protein_id="HEG1169005.1"
                     /translation="MDVLLHNPLWFYLFIVNFYLFCLMGYDKHQARTSGWRVPENNLI
                     FVSLLGGGLGGLISRKVFHHKTRKKKFTIGFAAGVLVDLILIYFFH"
     gene            26888..27934
                     /locus_tag="SCJ86_001461"
     CDS             26888..27934
                     /locus_tag="SCJ86_001461"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306568.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flippase-like domain-containing protein"
                     /protein_id="HEG1169006.1"
                     /translation="MSSKGTKTKIILNLLLLIVIFAIIYYLINQSFADIFAELLSTSV
                     VVLAAAVILGTVYQIAEGRSIKEIAEPFAPNFTTKDGFLTSSYIAFYRIVSFGTGTLI
                     SEIYFYNKKGMKLSQGAGVTALHMIMYKLAVITYALAGLVIQFSLFYSNGPSMIWFII
                     AGILLTIVIVAFLLAMSVSLNVQIFFVSLSNKLFKSERLRKWVDTCNQQIYSLRETVQ
                     TIIQDRTALLRIYLWNMLKLVFWYVLPYLILIENHPKIDFLLTISFISFAVVLSGVIP
                     TPAGIGSFEFVYLLLFRPVVGTVDAVSSLLLYRFSSFILPFLYGFVYVIAERRRVIKE
                     EIIEVKKENKENQG"
BASE COUNT         9411 a         4844 c         6103 g         7650 t
ORIGIN      
        1 agatactctg attgagcgat ctacatttct atatgttcaa ttgcaggaaa ataaaacatt
       61 gctgcatttt tcaccagagt tccatctaga ggggctaaca ttaggagaaa tctatcaagc
      121 agaagggctg tcggctgttt tgcacttttt taaggtagaa ttggaattac cagtaaaaga
      181 ttatatcgag ctttctcttg aatcttggga tcgagcagtg gttgaactgt ttccagaagg
      241 tttaatgatc gatacaaacg atgggaagat ccatctgact gcagcagaac tgcaaaaaca
      301 aatgcgttat caaatagatg acgaaaattc tttttctatt tttagacgtc agcaaaagat
      361 tctaaaaagc tttttaaaag tgatcagtaa aaaacggaat ctgatcaaaa cgacacagct
      421 gttgaaaaaa tatccaggtc tgcttcacac gtctattcaa tttcctcaat tgattacgat
      481 gatccatcgt tttatacgta tgcaacagct accatctaag aagctgtttc ttccattgac
      541 ggacacattt cgagtagtca acagagaaga taaaaagaaa gtgattgtga ttgactttac
      601 cagaaatcgg aatgtccttc accaagttgc aatggaaaag gccaattaag gctttttctt
      661 tttggatttt tttgtcataa taaatattag atgatggagg agataatatg atgcaaacat
      721 ggttagaaag gctaatacga tttcagcaga actatcacaa acttatgcag ggaagatatg
      781 cgaaatttga tcagttgaat cgcggtctat tgatcgctgc gcttgtcttt agtttgctaa
      841 gtcgttggtt gccctataga atcggacaat tgttgttcat cgtctttttc ggatggatga
      901 tatttcgttt cctatcaaaa aaaatctacc ccagactaaa tgagaatcaa cgttatttga
      961 aatatcttga tagagtcaaa gaaaaatatc atcagataaa aaacaaaaag caagtcaaaa
     1021 tggatcgaaa aagttatact ttttttgagt gcccgaattg tcatcaaaag cagcgtgcac
     1081 ctaaaggaaa aggacgaata cgtgtgacat gcaaaacatg tggtataaaa ttcgaaacga
     1141 atgtttaagg gagaaaagaa tgaaattagt attattacgt catggcgaaa gcgaagcaaa
     1201 ttttgaaaat tactggacag gatggctaga tgtcgcctta acagaaaaag gaaaagaaca
     1261 agcagcagtt gccggtcaaa aaatgcttga agccggactt caattcgatg ccgtttatac
     1321 ctctgttttg aaacgtgcga ttcttaccgg acaagtcgca ctagaagaaa tgggtcagtt
     1381 atggcttcct ataataaaaa catggcgtct aaatgaacgg cattatgggg atcttgtggg
     1441 taaaaacaaa gataagatga aagaaatata cggaaaagat caagtaaaaa catggcgcag
     1501 aggatatacg gaagttccgc cgttaaccgc agaaaataga tttgaccgca gatatgatca
     1561 cttggatccg catttgatcc catacggaga aagtctggaa atgacagtga atcggatcat
     1621 tcccttatgg caagatcatc tagcaccaaa actaaaagat cggcaagatt tactagttgt
     1681 gggtcatggt aatagcatcc gagcactgac aaaatattta gaagatgttc ctgaagatca
     1741 aatggatacg atcgatatcc caaatgcaca accaatccag tatacattag cagatgattt
     1801 gacaattctg gacaaaaaaa tactttaaag aacaccttta tctaaaagtg agagatagaa
     1861 aaaagaagca aaggaatgga acaaaaggaa catgaaaaat tatatatggg actttgatgg
     1921 tacattgttt gatacgtatc ccgcaatggt agatggtgca tggcaagcac taaaagattt
     1981 tggtatcagt atggacaaaa aagaaatcta cttcaaaatg aaaaagtatt ctactagtta
     2041 tttgatcaat gaatcaaact tgaatgccag agagtttaat gaattatttc accgatatga
     2101 aaaagaaagt acggaagttt cccgtccatt tccagaaacg aaacaagtac tggaaatgct
     2161 aaaagacaac ggcggtcgtc atttcatcct gacccaccgt ttgacagaat caacatgggg
     2221 cctgttaaaa gaacatcgat tggcacatct gattgaggaa gtagtaggga tcgatcagga
     2281 ctttcctcgt aaacctgatc cagcatcttt aaattatttg atcgatactt ttcatttaga
     2341 aagaacagac acgatgatga tcggcgatcg tcgtttagat atcgaagcag ggaaaaatgc
     2401 aggagtcgct acttgtcttt acgacatcga tcattttctg ggtgagatcc cggcagatta
     2461 tgtggtagga aatctgaatg aaatccttac cttgcagagc atcattaaaa agtagtatcc
     2521 cctttcaaag gagatatgtt ataattttca taaaggagtg ataaaattat atggaaatca
     2581 gaagacaaaa agaagttgta gaagcatttc attatgatat gagattgccg aaccaagaag
     2641 tagaaacaga tttgaaagta ggcttctcgc ctttaaaaga aacgatcgaa aactatccga
     2701 aagaacatag catcgtagga gcacgcttag agttccgttt agtgtttgat acatatgttt
     2761 tatctggaag tgtgagtcag atcaatcata tcctcaatcg gaaaattgaa ggtcaaaatg
     2821 atgtcacaca agaagaagtg gacgagctag tagcaccgtt attcgatatc gtacgccgaa
     2881 tgtcttatga ggtgacagag atcgccttag atcagcctgg ggtaaaattg aattttcaat
     2941 cagaaaaaca agaatagaca gtaaaaacat acctgcgata gattaaagct atcacaggta
     3001 tgttttttta ttactaattg taagcaaaaa gaatgtcatt tcgataaaaa tcgtctaaaa
     3061 tataaaagtc actgaaaaaa attttttcag gaggatggtt gaagaatcat tctcgggaac
     3121 ctttacttcc ttcgatggac ttgtcattaa taatatgagg aggagaaaaa tgacaacact
     3181 catcacagtt ttagtggttc tagtttttgc ggcattatta tatgtgttct atcgtatgca
     3241 aaagcgccat acaaaatttt caacacgcgt ctttacagca ttaggagttg gtatcgtctt
     3301 tggcggtctg cttcaattga tctttggtgt agacgacaaa gtcgttactc aatcaatgga
     3361 atggatcggg attgtaggtc aaggatatgt atcattcttg caaatgctag ttatgccttt
     3421 agtgtttatt tcgatcattg gtgcattcac aaaaatgaaa gtttctgaaa agctagggaa
     3481 aattagtgcg actgtattga cttctttact tggaacaaca gctattgcag cattgatcgg
     3541 aattggttca acaatgctgt tcggactcca aggagctaaa ttcacacaag gagctgcaga
     3601 aacagcacga atctctgaac tggcaacacg tcaggaaaca gttgaaaact tatcgattcc
     3661 acagcagatt ttatccttta tccctaaaaa cgtttttgct gatttagcag gtagtcgtgc
     3721 aacaagtacg attggagtcg ttattttcgc ggcatttgtc ggaatcgctt atctaggcgt
     3781 gaaaagaaaa gcgccaaaag aagcagcatt cttttcagat ttagtcgaca gtctgtataa
     3841 aatcattatg cggatcgtga cgcttgtttt gcgtttgaca ccttatggag tatttgcttt
     3901 gatgacaaac gttttggcaa caagtgattt tgcagctatc gtcaatttag gtaaatttgt
     3961 cgttgcttct tacgtagcat tgcttgctgt tttgattgtc cactcactga ttttgattgg
     4021 tgcaaaagtc aatccaatca actatttcaa aaaagccttt ccggtattga gctttgcgtt
     4081 tacttcacgt tcaagtgctg gagctttgcc attgaatatc gagactcaga caaaagcttt
     4141 aggagtagat gatgcaacag ctaatttctc agcaagtttt ggtttgtcta ttggacaaaa
     4201 cggctgtgct ggcgtttatc cagctatgtt ggcaacgatc gtcgcaccag cagctggagt
     4261 gaatgtcttt agtttggaat ttattttgat gcttgtggca attgtaacaa tcagttcttt
     4321 tggtgtagct ggagtcggtg ggggcgcaac atttgcttca ctgatcgttt taggggcaat
     4381 gaacttgcca gtagcaatcg ttgggttagt tatctctgtt gaaccattaa tcgatatggc
     4441 acgtacagca gtaaacgtca gcggtagcat ggtagctggt atcgtgacaa gtgcacgcat
     4501 caaagattta gatcgcgatg tcatcaatga tgattctaaa gtgatcgatg tgaatgcata
     4561 gtcaaaaaga ggttgtgata gaagtggtca gctttaagaa ataagaagaa gtttcctaaa
     4621 aatagtttct caaatttttg tgaaatttcg gcttatttcc gagaagctac ttctgaaaca
     4681 ccgtgaataa gaggttgtga aaaagctgcc ctgcatcaag taataagaac agccaaacaa
     4741 aaatagttcc tcatatttta cgtttggttg agcttattac cgcagatgca agcttttgaa
     4801 caccgtttaa gaaagaggtc gtgagcctgc cgtttcactc tgagtcacaa agagcaattt
     4861 caaaaaatag cttctcatgt ttgttgaata ttgtgtcttg tgaccgagga gttgtcagga
     4921 gaacaccgtt taccagaggt tgtgacttgt ttttagtcac gacctctttt tttatttatt
     4981 ccatataaat acacattatt tattccgaaa agtgaaagcg caaataacaa aaacaaccaa
     5041 cttatttatt attaaagaaa agcgtattta agtaggacga ttctgttgag agctttcgta
     5101 gattttccca ttttcctatg gtaaactgac aaagttgaga atgttagaac agatcgttta
     5161 ggaggaaaca gatgtttacg aatgaaataa aaatcatgct ttatgtggac gatgtagaaa
     5221 aaaatgcaac gttttggaga gcagttggct ttgcggaaaa agatcgtcaa gaaatggatg
     5281 ggacgcttat aattgaagta ggagttgagg acagtcaaac ctcatttctg ttatatgatc
     5341 gagcatttgt tgagtcccat tcacctggca cagctactag tgccccatct ttgatgtttt
     5401 acagtaaaga tatctttgga ctatataaaa aaatgcagac gcaatcagtc acattaggtg
     5461 atatggtaca gcttggagaa gaatatgtct ttaactttgc tgatcccgat ggcaactact
     5521 ttgcggttac aggtaaataa tagaaataaa gactttgttt ttctgtgcga aacaggaaag
     5581 cgaagtcttt ttaattcaaa taataatatt atgtaaacaa aaaactttcc atgcgtaatt
     5641 atacacaagc aaataaatcg tcaaatgcga acgccttctt tatactgttt aggaacgaaa
     5701 agaaggaagg aagaaataca tggattatca agtactgctt tattacaatt atacaaaaat
     5761 cgaagaacca gaggaatttg ccaaaaatca tttagccttt tgcaaatctt tggatcttaa
     5821 aggtcgtatt ctagtagctg aagaaggaat aaatggtact gtgtctggaa ccgttgaaaa
     5881 cacgactgcc tatatgaaag caatgaaagc tgatcctcga tttgctgata cgatgttcaa
     5941 gatcgatcca agcgaaggac atgcctttaa gaagatgttt gtaagatacc gtccagagct
     6001 tgtaagttta aagctagaag aggacattga tccaaaccaa gtgacaggtg cctatctttc
     6061 accgcaagaa tttaaagaag ctattttaga tgaacagaca gtggtgattg atgcacggaa
     6121 tgattatgaa tatgacttgg gacattttcg aggggctgta cgcccagata tacgcagttt
     6181 tcgagagctt ccagactgga tccgcgagca caaagaagaa tttatggaaa aacgggtagt
     6241 gacctactgt actggtggca ttcgttgcga gaagttttct ggttggcttg tccgtgaagg
     6301 attcaaagat gtcggacagc tgcatggcgg tattgctact tatggtaagg atcctgaagt
     6361 ccaaggagaa ttatgggacg gcaagatgta tgtgtttgat gaacggatcg ctgtcgatat
     6421 caaccaagta gataaacaaa tcgtcggtcg cgactggttt gacggaacac catgtgaacg
     6481 atatatcaat tgtagcaatc ctgagtgcaa tcgtcagatt cttgcttcca ttgaaaatga
     6541 agaaaaacat ttaggagcat gtagcataga gtgtgcgaaa catccgcgca atcgctatgt
     6601 tcaagaccat ggaatctctc cagaagaagc agaactgcgg tttacagctt taattaaata
     6661 gaaaacaata gttttgattg tcgaccattt ccaatatgcg ctatactagc gttgaggatg
     6721 gtgatccaca tgaaatcatt gatttatgct attggggatg tccatggcaa acatgagctt
     6781 tttgaaaaac ttattcacta ttatgatcca aagatccatc agcttgtttt attaggggat
     6841 ctcaatgatc gaggtcctcg tacaaaagaa tgttttttat taggaaagca attagtcgaa
     6901 gaaacaggag ccatttacct ccggggaaac catgaagaat attttttgca atttctgcaa
     6961 tcaccggagg actggtatgc gccttatatg agaaatggtg gaaaagaaac gtttgaaagc
     7021 ctgattcacc caggggcatc ggaggaatac tccccaactg aaatctcgat gatgatccaa
     7081 tcgagatatt cagaactgat taaattttta gtccagcgcc ccttgtatta tgaatgggaa
     7141 aattatcttt ttgtccatgc cggtgtggat ttgacaaaga aagactggcg taagacaagt
     7201 ctaagagact ttatttggat aagggaacca tttcatgaag ggaaaaacaa tacaggtaag
     7261 acaattgtat ttggacatac gattacacca atgctccatg gagatatgca gacaacagac
     7321 ctctggattc aggatcataa aatcggaatg gacggtgggg ctgtattcgg agggtcattg
     7381 catggggttg tttttgacaa ggaaggtatt cttcaagata ttgaatatca gaacaacgaa
     7441 ggaccttggc atggaatctg actaatggaa agaatgaagg agctgtgact ttgttgcggc
     7501 tctttttatt tttatagaga atattgtttc tttcagtagt ttaacccagg ataaaaagta
     7561 tgatagaata aagaagcaat ttttagcaga aaagaggcat tttaatgacg gaaaatttga
     7621 accagacgat cattcagctt gtacaaaaag aactttcaga ctatcgtcct aagcagttga
     7681 ctactgtact taatttgtta aatgaaggaa acactgttcc ttttatcgcg cgttaccgaa
     7741 aagaaatgac tggaagtctt gatgaagtcc agattcgtga aatcgaagag cggtatgcct
     7801 atcttgaaaa cttagaaaaa cgtaaaactg aagtgattcg gctaatcgat gaacaaggga
     7861 aactgactcc cgagttagaa gcagaaatca cccaagccgt gaaaatgcaa caagtggaag
     7921 atttgtatcg tccatacaaa caaaaacgac ggacaaaagc aacgattgct aaagaaaaag
     7981 gacttgaacc actcgcgaag tggctgatgc agcttacaga tggagaggtg caatctgaag
     8041 cagaaaaata tattgacaaa gaaaaagaag tctcttctgc cgaagaagcg ttgcacggtg
     8101 ctcatgagat tattgctgaa caagtcagcg ataatgcaaa atttcggact tggattcggt
     8161 cttatacgta taacaaaggt atgtatgtca gcaatgtcaa ggatgagcaa gcagatgaaa
     8221 aaggcgtata tgaaatgtat tatgactttg cagaacccgt ccataaaatg gtttctcatc
     8281 gaatattagc tactaatcgt ggagaaaaag aagatattct gaaagtattc tttcaagtag
     8341 atgaagcagc aattttagct tacttggatc gccaaatagt caaaaatcct gcctctcctt
     8401 cttcttcatt cgtcagagaa gcctatcagg atagttataa acgctttatt cagccagcaa
     8461 tcgagcgaga actgcgcaat gaattgactg aaaaggcaga tgagcaagca atcgctattt
     8521 tcggcgaaaa cctccgtaat cttttgttgc aaccgccact aaaaggtaaa gttgtcctgg
     8581 gatttgatcc ggcttatcgt actggatgta aactagcagt cgtagatgcg acagggaaag
     8641 ttttagcaat cgaagttatt tatcctcata aacctgcagc gcaagcaaaa agagaagctg
     8701 ctggaccagc ttttatccaa ttaattaata aatatcaagt agatatggta gctatcggaa
     8761 atggtacagc cagtcgggaa tctgaacttt ttgtggctga gcagttgaaa tccgccgacc
     8821 ataaaaccta ttacgctatc gtcaacgaag caggcgcttc tgtttactcc gctagcgaaa
     8881 ttgcacgaaa agaatttccg catctacaag ttgaagaaag aagtgctgtt tctattgccc
     8941 gtcgcctgca agatccttta gctgaattag tcaaaatcga tcctaaagct gtcggagtag
     9001 gccagtacca acatgacgtt tctcaaaaac gtttggctga acaattggat tttgttgtgg
     9061 aaacagcggt taaccaagta ggtgtggatg tgaatacagc aagcccgcag ctcttgcagc
     9121 atatatcagg attgaataaa acaactgccc aaaacattgt catttatcgt gaagaaaacg
     9181 gagaattcac cgcacgaact caattgaaaa aagtgccacg cttaggacca aaagcatatg
     9241 aacaagcaat cggattcttg cgagtccctg gtgggaaaaa cattttggac aacacaggta
     9301 tccatcccga aagttattct attgcaaaag aaatattaac ttcagtccaa ctttcagaaa
     9361 aagagcttgg tacagaagaa gcagtagaaa aattgacacg attatccgta gagaaactgg
     9421 ctgaaagcct atcagtcgga gaagaaacgt tagccgatat tttagctggg ctgacacaac
     9481 ctggacgaga tatgcgtgat gagatgccag cgcccttatt acgtacagat gtattgagta
     9541 tggaagactt gaaaccaggg atggaattga ctggaacggt ccgtaatgtc attgacttcg
     9601 gcgcatttgt tgatatcggt gtgaagcaag acggacttgt ccatatatcg aaactcagca
     9661 aaaaattcgt caagcatcct acagatgtcg tgtcagtagg agatatcgta acagtttgga
     9721 tagaacaagt cgatacgaaa aaaggcagaa tcagtttgac gatgctttcc ccttatgaag
     9781 agtaacaatg aggagtaaca aaaattgaca caagaagaac tgcagcagtt agtagaagag
     9841 atatcattaa aaagcttcca gcttcctttc aagcaccaag caatgttcaa tccacgctta
     9901 cgtacaactg gcgggcgcta tcatttaaac gatcatcatt tagattttaa tgcgaagcta
     9961 tttgaagagt cagacgaaga gacgattgca ggaatcatca aacatgaact ttgccattat
    10021 catcttcatc tagctggaag agggtatcag cataaagata tggatttcaa gtatcttttg
    10081 aaaaaaacag gagggttgcg ctatgcacca gccttgacga agaaacagat aaagatggag
    10141 tattatcgat gccaagattg ttctgccacg atctgccgca agagaagaat cgatgtacgc
    10201 aaatatcgtt gtggaagatg tcatggagaa atcaaatgga ttgagacaaa aagagaagac
    10261 tttaatgaag cacaaaaaat cagatgaaag gatagaaggc ctttggaaaa aagtgagcag
    10321 aggaatctaa aagactggaa tcagctatta aattttttag ccgcacctga agagggaaat
    10381 tttgaggaac tgacggcacc tacacttatt ttagctggaa actgtcttcc tatcttagct
    10441 gataaagcag cacaaatgta tttaaatggc caagtagatc aattgttttt ggtcggaggg
    10501 gtggggcatg cgactagaat cctttatgag aattttgaaa agcaaggatt tcattttgaa
    10561 gatggcatga gtgaaagtga aatctgtcgt cagtatctaa aagaagtcta tgatttgccc
    10621 gataaggctt ttctgataga atcgaagtct acaaattctg gagagaacgc cactttttca
    10681 ctggagattc ttcattcctt aggagctgta ccagaaaaaa tcttattgat gaatgatccg
    10741 acactgcaac gcaggactcg agcaactttt gagaaagtat ggcaaaatga acaaactatt
    10801 ttcgcgaatg ctgtgccatt tgtgccggaa atccttcact tttctgaaga gatcatcttt
    10861 acagcaaaag aactgaatca tcaatggcct aaggaatatt tccatgcgct agtattagga
    10921 gaaatggaac gtctccatga cgatgagaac ggctatggtc cgaaaggaaa agattttatt
    10981 cctcacattg atatatcaga ggaagtttgg tcttcttaca ccagaattaa agagtcaata
    11041 aaaacggatt tttctcgtac ttgaccattt tagcaagcga atcctccttt ccaaataaat
    11101 ttggttgaaa ttttcaatcg tttataagat aatgaggagc gtgttgaatg taatagctaa
    11161 aagaatggac gctagataag agaagaggat tcaaatggaa aatcaaaata aattgtatca
    11221 ttttgttggt atcaaaggtt caggaatgag ctccttggct cttgttttac atgaacaagg
    11281 tctaaatgtc caaggatcag acattgaaaa atatttcttt acacaaagag atttagaaaa
    11341 agcaaatatc acgattctgc catttaacgc agataatgta aaaccaggga tgacgatcat
    11401 tgcaggaaat gcatttcctg attcacatga agaaatccag cgagcaaaag aattaggttt
    11461 ggaagtgatt cgctatcacg actttattgg tcattttatc caaaattata cgagtatcgc
    11521 tgtaacaggc tctcacggaa aaacaagtac gactggactg ctctctcatg tattgagtgg
    11581 tgtccgtcca acaagttatc taattggaga tggaacagga catggtgatc cgcaagcgga
    11641 attcttttca tttgaggcct gtgaatatcg ccgtcatttc cttgcctatt caccagacta
    11701 tgcaatcatg accaatatcg attttgatca tccggattat tatacaagta tcgacgatgt
    11761 atttacagct ttccaaacaa tggctggaca agtgaaaaaa gctatttttg cgtatggtga
    11821 cgatgcttat ttacgcaaat taaaagcaaa tgtgccgatt tattattatg gtgtcacaga
    11881 aaacgatgat atccaagcac gaaacatcga acgcacaact tctggttctg cttttgatgt
    11941 ctatcatggg gatgaatttg ttgggcactt cactgtacca gcattcggta aacacaatat
    12001 cttaaatgct ttaggtgtta ttgcagtagc ttatttcgaa aaactggatc tgaaagaagt
    12061 tgcagaagaa atgctgactt tcccaggagt aaaacgtcga ttcagtgaaa agatcgtagc
    12121 agacatgacc gtagtcgatg attatgcgca tcaccctgca gagatcaaag caacgattga
    12181 tggcgcacgc caaaaatatc cagataaaga aataattgca gttttccaac cccatacgtt
    12241 tacgcgtacg attgcattga tggatgaatt tgcagaagca cttgatttag ctgataaagt
    12301 ttacttatgc gatatttttg gttcggctcg ggaagaacaa ggaaacgtta agattgaaga
    12361 ccttggagcg aaaatcaaaa aaggtggaga agtcatcaaa gaaaataatg tatctccatt
    12421 gttagattat catgatgcag tagtaatctt catgggtgct ggagatgttc aaaaatttga
    12481 acaagcgtat gaaaaacttt taagcagcac gacgaaaaat gtactgtaat acaattactg
    12541 cagtgtcctt ataaaaatag aaagaggggg agtggcaaaa atcttgccaa gccccctttt
    12601 ttgcaactta tgataaacgg tgttcaaaag cttgcatctg cggtaataag cccaatcaaa
    12661 tcaaaaacat gagaagctgt tttttcttgg ctgttcttat tatttgatgc aggacagctt
    12721 tttcacgacc tctgtaaact ataggaatct gttaaatcaa gggagcagaa cttgcctttt
    12781 acgctacttt tggtacaata atgtatgatt aaaaataaac tggagggttt ctatcttgaa
    12841 cataggtaag ccgagaaaac taggaaaaac aataaatgaa atcgaggaag gcgactcgtt
    12901 atccttaaca gaatctattg aagataacca gcttttgctg tatttgggat tgacgaatga
    12961 tgcaaatcct ttgtatatcc aacatgactt tgcacaaaaa acagaatatc aacagccaat
    13021 cgttccatca gtgatgctga tggggatcat cacaagtgca atttcaaagc atcttcctgg
    13081 accaggatca catgttgtca atttttctgt caattttatt gaaccggttt tccattatga
    13141 aacactgact tttcagtttg aggtcataaa agtagataag atgaaggatg tggtgacgat
    13201 ttctgtagaa gcggagaata cgaacgaaga tcgtgtgttg gatgctgtcg ttatggtcca
    13261 accgccacaa gttcctgaat tggaaatagc aatcgaaagt gaaggtgaat tgaatgaata
    13321 atccaagtac atcaatgtca ggtatcaatc gtttttatgc aaaagtttat ggatttttag
    13381 gtatgggact tgcccttagt gcagtcactt cttacttggc tctttgggta tttcctttgc
    13441 aattagcaac ttttatcaat aattttccac taggctttgt gggtctgtgg atcgttgaac
    13501 tgattttagt aatggttcta ggcgtaaaag cacaaaagaa tccgagctta gcaatcggtg
    13561 gatttattgc ctactctctt ttgaatggca taatattagc ggttactctt gcatactacg
    13621 atattggaac agtaacgcaa gctttcttga cagctacttt gatgttcatg gggatgtctg
    13681 ttgtcggagt aacaacaaaa agagatctta gcggggttgg aagagcagga caggcagctt
    13741 taatcggagt gatcattgca ctagtcttga acatcttcat tttgagaagt agcgtagttg
    13801 agctgttcat ctccctactg ttagtcgtca tctttgcagg gattacagct tatgataacc
    13861 aaagcatccg taatatctat gtccagacaa acggaacagc acatagtgga attgctgtat
    13921 tcatggcatt gaagctgtat ttggacttta tcaacttgtt cttggcgttt ctgagaatct
    13981 ttggacggaa caactaaaaa agaaaaaaat tcatcagttg gggctatacc gaattatttg
    14041 gtatggtccc gctttttctt ttcgcattcc gcaggattct ttgttagaat gaataagaac
    14101 tttacagtaa ggagttaaga agatgacaaa aaataaatta ttactcgttg atgggaacag
    14161 tgtggctttt cgcgcattct ttgcgcttca caattctctt gaacgtttca aaaatagaaa
    14221 cggtcttcat acaaatgcaa tctatgcatt caatacgatg tttgaaaatg ttatgcaaaa
    14281 agagcaaccg actcatgttc tggttgcgtt cgatgcagga aaaacgactt ttcggacaga
    14341 aatgtatgcg gagtacaaag gtggaagatc caaaacacct ggcgaattca aagaacaaat
    14401 gccatatata cgtgatttgt tgacaggact aggggtacag tattacgaat tgcccaatta
    14461 tgaagcagat gatattatcg gtactttggc agaaaaagta gacaaagatc aatttgatgt
    14521 cgtcgtgctt tcaggtgatc gggatttgac tcaattagct agtaaagaag taaaagtgga
    14581 tatcactgta aaaggtgtca gcgatatcga aagctataca ccagaacatg tagcggaaaa
    14641 atatgatggg ttgacgccaa aacagattat tgacatgaag ggactagccg gagatgcctc
    14701 tgacaatatc cccggtgtga ctaaaatcgg tgaaaaaaca gctatcaagc tattgaaaca
    14761 atatggctca gtcgaaggaa tttatgaaca tatcgatgaa atgaagcaaa gcaaaatgaa
    14821 agaaaatctg ataaatgaca aagagcaggc tttcttatcg aaaaaattgg cgaccatcaa
    14881 tacttcagcg cctgtggatg tctctatcga ctcattgaag tatgaaggga aaaatctcga
    14941 taaactcgtt ccgttttata aagaaatgga tttcaatcag ttcctttcta aattgaatat
    15001 cgtagaagaa gaagtaaaaa tggatgatat tttgtttgaa gtcgttcatg aattcaaaga
    15061 ggaaatgttt actacggata tggcgttgta cgtggaaatg atgggcgaca attatcatac
    15121 agaggaaatc gtcggagtag cgtgggggac agaaaagaaa atttatgtca cgaacgatct
    15181 atctgtattc gaaaaccgag cgttccattc ttggatcact gatccgactc ggctgaaaaa
    15241 agtatatgat gcgaaacgaa cttatgttgc tttaaaccgc tatactggaa aaacgaaagg
    15301 aatcgatttt gatgtcttgc taggtgcata ccttctcgat acgaatgaaa aaagcacaga
    15361 tatcgaagga atagctgctc attacggata caacgatatc caatcagatg aagctgtata
    15421 cggcaaaggg gcgaaaaaag gtttaccaga agaagaggaa ctcttttttg ctcatttagc
    15481 gcgaaaagta gctgcaatca atgcgttgac accaaaattg agccaagaac tggcagaaaa
    15541 aaaccaagaa gatcttttcc ggaaaatgga actgccattg tctatcattt tggcagagat
    15601 ggaaataagc gggatcaaag tagatgcgac tcgcttacaa gaaatgaagg gcgagttctc
    15661 tgctcgctta agagagatcg aacaaaaaat ctacgaagaa gccggcgaag aattcaattt
    15721 gaattcacca aagcaattag gagtgatctt atttgaaaaa atgggattac ccgttatcaa
    15781 aaaaacaaaa actggatatt ccacagccgt agatgtactg gaacagcttc gtgaacaagc
    15841 accaatcgta gaggatatcc tcacctatcg tcaaattgca aaaattcaat ctacctatgt
    15901 cgagggactg ctgaaagtga tccaatcaga tggaaaagtg catacccgtt atgtccagac
    15961 attgacccaa acaggacgat taagctctgt tgatcctaat ttgcagaaca tcccgattcg
    16021 attagatgaa gggagaaaga tacgtcaggc atttgttcct agagaaaaag actggctgat
    16081 ttattcttct gactattcac aaatcgagtt acgcgtactt gcgcatatct caaatgatga
    16141 acatttgaaa gctgccttcc tagaaggtca agatatccat gccagcacag cgatgcgtgt
    16201 atttggaatc gaaaaggcag aggatgtcac accaaatatg cgtaggcagg caaaagcagt
    16261 gaattttggt atcgtgtatg gcatctctga ttacggactt tcacaaaatc taggaatcag
    16321 ccgaaaagca gcacagcaat acatcgatac gtactttgaa aaatacccag gtgtcaaaga
    16381 atatatggaa agaatcgtac gcgaagcaaa agaccaaggc tacgtcgaaa cgctgtatca
    16441 ccgccgtcga tatctgccag acatcaattc aagaaattac aatatccgtt cttttgcgga
    16501 acgaacagct atcaatacac caatccaagg aagtgctgct gatattttga aaatcgcgat
    16561 gatcgaattg gataaacgat taaaagaaac aggtcttcaa gctacgatgc tgttacaagt
    16621 tcatgatgaa cttgtcttcg aggtaccgga aaaagaactt gaatcattag ataaattggt
    16681 gaaggaagtt atggaacaag ctgtttctct tcatgtacca ctgatcactg acagcagttg
    16741 gggaaaaacg tggtacgaag caaaataaac attgtttaag acagagcaat ataatgaagg
    16801 tgaaaagaaa aaatgccaga attaccagaa gtagaaaccg ttcgtaaagg attggaacgg
    16861 ttagtagtag gaaaaacgat tcaaaaagtt caggtcttgt ggcccaagat catcgagcaa
    16921 ccagagacac ccatctttga ggcttctctt gttggcgaaa ccatccaatc cattggacgt
    16981 agagggaagt ttttaatttt tcatctcgat cattgtgaac tgatctcaca cttgcgaatg
    17041 gaaggaaaat atcaatttac gaaagaaaat acaccaatcg ataaacatac gcatgtactt
    17101 ttcttttttg aggatggaag ccaacttcgc tacaacgatg tacgaaaatt tggtcgtatg
    17161 acaatcgtcg aaaaaggagc aagtgcaact taccgaggaa tcatgaaatt gggacctgag
    17221 cctttgccag atagcttctt attagcagat ttcgcaaatg gactgaaaaa gtcccacaaa
    17281 gcaatcaagc cattgctgct agaccaacgt ctagtgactg gtctaggtaa tatttatgta
    17341 gatgaagcat tatgggaagc gaagatccat ccagaacaac cggcgaacac actgcgcccc
    17401 aaagaggtag aacaattacg tctatcgatc attgatgttt tggatcgtgc aatagaagcc
    17461 ggaggtacca cgattcgcag ttatttgaat gcattaggag aatcaggcgg atttcaagta
    17521 gcactgcatg tctatcagca aactggcaaa ccatgtatac gttgtggaac gccgatcgtg
    17581 aaaacaaaag tggcacaaag ggggacgcac tattgtccga attgtcaaag gctaaaaatc
    17641 gcaaagtagg atttgtttta ggtttgacgg gtggaatagc taccggtaaa agtacggcag
    17701 ccaaagtctt tcagtctcat ggatttcctt tgatcgatgg cgatgtgatt gccagagaaa
    17761 ctgttgagcc tggaacgctt gctctgagga aaattgtatc agtgtttggc caagaggtat
    17821 tgcagcctga tgggcaacta gatcgaggga aacttgggat gattgttttt ccatcaaagg
    17881 aaaaacggca aaaacttgat cagctattag atccgtttat ccgaaaagcg ataaaagatc
    17941 aaatcgcctc tctaagttcc aatcatcctt tagtaattgt agatatccca ttgctctacg
    18001 aaggtcatta tgaccactat atggatgcag tagctgtcgt ttatactacg ccagaaactc
    18061 agcttcagcg tttgatgaaa cgtaatcaac tgacgctaga acaagcgcag cagagaatta
    18121 gtagtcaact tccaatcgaa gaaaaaaagc agcgtgcgga tattttgttc gacaacaatg
    18181 gaacaaaaga aatacttgtg ggacaaatcg aaaattggct ttttgctaat cgctttcttt
    18241 gagtatctgt gtcaattttg ctatcctgag tatatagaaa gtgagggata gcgatgggat
    18301 cattaaaaaa tcaaaaggat aaagtcgtag gtaacgcaaa agaaaaaatc ggcgagatgc
    18361 tcaataaaga aaatctaatc gatgaaggtc gtacgcaatc agaaacagca gaagaaaaag
    18421 aaaagcagta ccaagcaaga atcgatgaaa tagaagacca gcaggcagaa gaagaagcga
    18481 agcggcaaga tttgactcaa gaacgaaaaa atccgacacc tgatcgtagt aaaacaccgc
    18541 tcccagaaag tgaccacatt cccgatgagc aaaaaatcga ggaacagcga atgaaacact
    18601 atacaggaat cgaagagaag ttataaaata gtaaaaaatc gagatgtgac ttgaagcaga
    18661 tgagaaggtg ttcatctgct tcaagttttt tgtgctagat gactaaatca gcttttgtgt
    18721 aagttatttc tcagtaatca aggtgatcag tattggaaaa ttgaaattta gtaaggacag
    18781 gggacacaat tacgcatgaa agtagaaaaa attgttctta tatccgtgtt ttttgggtag
    18841 gacagtcgcg tttgtgattt gtttcgtgtt ataatacaac tagttgtaaa gaaagacaca
    18901 tcctttatat aaaatgcgat gcaagaggtg agacaaaatg agatgtccca gatgtcaaca
    18961 taataattca cgagtaatcg acagccgtca agcagatgat ggacgtgcga tccgtcgccg
    19021 tcgcgaatgt gagaattgtt cgttccgatt cactacattt gaacgaattg aagctgctcc
    19081 gttattagta atcaagaaaa acggggaacg cgaagagttc aatcgcgata aaattttaag
    19141 aggactcatc cgttcagcag aaaaaagacc tgtagctatg gaacaaatgg aacaaatcgt
    19201 caaccatgtc gaaaaccgcg tacgcagtct cggagaaaac gaagtatcga cgaatctaat
    19261 tggtgaatat gtgatggaag atttagttga tttagacgaa atcgcttata ttcgttttgc
    19321 aagtgtttat cgccagttca aagatatgtc tgttttctta aaagagttgc aagatatcgt
    19381 ggataaagca aaagaaaata ataaagagaa taataaagaa aacaattaag aaacaaaaag
    19441 gaggcatttc cttggaaaat gcttggaaag agcttcaacc aaaaaatatt taccaaggaa
    19501 gaaaagcggc tccgctgaga gaagacagca agaacgcctt gctgtatctt tatcagccac
    19561 taatcggcgg agaagccttg agtatctatt tcaccctgtt atcagaaatc tctcaagaat
    19621 ctggcaaagg tcctgaaggg atgcatgcag atctgcttag tagtctaggc tgcggtatcc
    19681 cgcaatttta tgatgcaaga aaaaagctag aaggaatcgg cttactggat gtttatttta
    19741 aagaagatag tgatctggga cctcgtttta tttatgagct gttagaacca ctcattccta
    19801 aagcattttt caaagactct gttctggctt ttcttcttct ggaaaaagtt ggtgaacgac
    19861 gattccagaa gcttacccaa ttctttgaac ctaaaaggca tacagcagag ggatatcaaa
    19921 aaatcacaaa aaaattttta gatgtttatc gcttcaatga agcagtgtac gctgctaatc
    19981 aagaaaacat cgaatcgctg aataaatctt ttgaagaatc tgaatcagta gcattacttg
    20041 aagcagattc gacattcgat tggcagtttt tgaatgattg gctggcaaaa aaacatattg
    20101 caagtcttga tgatccaatc atcaaacaag tcgacatgta tcatcagctt tacggtttcg
    20161 atgaattgac acttggcgaa ctgatcagtc aatcttatga ctttagtgaa caaaaagtat
    20221 ctgaaaaaga actgcaaaaa gccattttgg caatgcagag acgtccggtg aagcaaacaa
    20281 aagaaacagt tgcggtttct gaaaacagta atgaaccctc gtcaaacgaa catttgacaa
    20341 acgcagcgct tcagctgata aaagaagcac agagcattcc accgatgaaa tatctggaag
    20401 caatcaagtc agaaaaaggc ggatatgttt ctaaacaaga aaactggtta ctgcaagatc
    20461 ttgtttctca atcaggtctt tccagtagcg tgatcaatgt tttgctgaat tatgtactag
    20521 tgatcaagaa taatgctagc ttaaatgcca gttatgtaaa tacagtggca aatgaatggg
    20581 cacaaaagaa aatcgccaca gcagaagaag cgatccagca cattcggcaa ataagtaatc
    20641 aagcaaaaca atcaaaacaa aagaagcaaa caaattatca aactggcaag cgaaatgttc
    20701 gacgggaaaa gcttcctgat tgggtcaacc agccaaaaga tgaaaaacag atcagcagcg
    20761 agaaaaaagc agagattgac cgccgtttta aagaatacct ggcaaaaaag gagggtgata
    20821 tctagtggaa gacgtaggca aagaattgaa gaggatcatc agcaaacgca attatcaaga
    20881 aaagtatgct gaaatgatcg ctgaagtctt aaatgatgcg gatgttcaag cttttctaag
    20941 cgcacaccaa gaagaattga cacaagcgga tattgaaaaa agttacgcta aattgtatga
    21001 attcgtgcaa gaaaagcgta aatatcaaaa aaacgatcca acgatgatcg ctcccggcta
    21061 tgagccgaag ctgacgttga attttcatta tgtggatgtc acttatgtac caacggaagc
    21121 attgttagca cggcaaagac aagaagaaat cagaagccga gtaaaagcaa tggatatgcc
    21181 aaaagatatt caatcggctt cttttgaaaa ctttgaacgt acagatggac gaggatatgc
    21241 gagcttagaa gccctagatt ttgtagagaa atatactgct gatccaaaag ggtatcataa
    21301 aggactgtat ctagtaggaa gttttggtat tggaaaaaca tatttattag gagcaacagc
    21361 gagagaatta gcgattgctg gattcaccac gacgctagtc cattttccga catttgccgt
    21421 tgagatgaaa caggcaatcg gaaaagatca agtggcagaa aagttagatg cagtgaaacg
    21481 ttcacctatt ttgatgatcg atgacattgg tgcagatgcg atgagtagtt ggatacgaga
    21541 cgaagtattc ggtgttatcc ttcaatatcg gatgcaggaa cagttaccaa ccttcttctc
    21601 atcgaatttt acaatggatg aattggaaca gcatttgtcc gtcactcaac gtggagatga
    21661 agagccgcta aaagcgaaac ggataatgga acgcatacgt tatttgacaa aagaaatcga
    21721 aatgactggt cgcaaccgac gcaatggttg aacagttgac aagtccaact gacatttgac
    21781 ctagtagtaa aagcaggtca gcgaatcatt atatggacag aaatgaccgg agacaatcac
    21841 cgcaacaagt aatgcagaaa cttaaacgga ggatttttta tgagggctat tttttctagt
    21901 atatctgtag ttggtctatt ttttctttta gcaggctgcg gtagcgcaga tagcaacaca
    21961 ggttctgttg attcgattga tgcgggtcaa acagtaaaaa caagcagtac gaaaacggag
    22021 agacaaagca tatttgtcgg aacgattgaa agtatcactc aagtagaagc agaagaagaa
    22081 acgcttcagt tgaaactcgt cgatgtcatg gccaaagaag atccagcgaa tatgggaacg
    22141 tcattcagca atgacggagt gatcttaaat gctactgctg aaaaaataag cgtggacata
    22201 aatgaattga aaatcggtga caaagtccaa tttacattag ttgagatgcc gatcatgacg
    22261 atgtctatcc caccgcaagt tcctggagat tcgattattg atgtttcatt gaacaaataa
    22321 ccaataacat tttttcaaaa ataagaaaat gttgttttat agaacgaaaa ggtatatttt
    22381 ttaaggagat atgggaattt ttttattcct agtctcctta ttttatttgg aaaatgctat
    22441 aattatctag aaggagtgaa gctatgaacc atttagattt atctcagcgc ttagccgcag
    22501 tgggtgactt tgtccctgaa tccgctcgtt tagcagacat tggatcagat cacgcttact
    22561 tacccgttgc attgatatta agaggaaaaa tcgaatatgc agttgctggt gaagtcgttc
    22621 aaggtcctta tgaatcagca cgtaagcaag tcaaaaaaga tggattagaa gaccgtatca
    22681 ccgtacgcct tgcaaatggt ctagatgcta tccagccaca agatggtatt acagctgtca
    22741 cgattgcggg tatgggaggt tcactgatcc gagatatcct agaagctggc aagttgaatc
    22801 aacgcttaag cggacaagaa cgtttgatcc ttcagccaaa catcggagag cgtacattac
    22861 gatcttggct gcaagaaaat cagtataaaa tccaatcaga aaaaatactt gaagaaaata
    22921 aaaaaatata tgaaatcatt gtagccgaaa aagaacaaaa accagttact tacaatgaaa
    22981 tggagctgat gttcgggcca tttctgcttg aagagaaaag tgagatcttc aaatcaaaat
    23041 ggacaagaga gcttcaacaa agaaaagcta tccgtgaaca gctcaaacaa gcagctaatc
    23101 aagaggcacg tcaaaaacaa atcgagcagg aaatccaaat gattgaggag gttttaaagc
    23161 catgacaatc agcggacgca ctttcatcca aaagtttgaa agctattgtc ctcagtggct
    23221 agctgaagaa ggagacccag tcggactgca tatcggaaca ttggataagc caatccaacg
    23281 agtaatgatg acattagacg ttcgtccaga agtggtcgaa gaagcaatca agaaaaaaat
    23341 cgatttgctg attgctaaac atcctccaat ctttcgtcct gtaaaacggc tggttactga
    23401 tcagccacaa gaaaaaatgt atgcagattt gttgaaacat gatattgccg tctatgcagc
    23461 acacacgaac atggatatta tttgggatgg actgaatgac tggttttgtg aacttctcgg
    23521 tattgaagtg gagagttatt tagtaaagac tcatgagatt cattataaaa aactggccgt
    23581 ttatgtacct gtggatcatg cacagaaaat gcgtgaagta ttagctgcag ctggtgctgg
    23641 tacacaaggt gattataccg gtacaagttt tacctcgatt ggacacggtc gatttactcc
    23701 ggaagctggt gcacaaccag cgattgggaa agtagggaag acagaacaag ttcaagaggc
    23761 aaaagttgag gttattttgc cggaaactat cgaaaaacaa gtgattcaag ccatgcggtc
    23821 agctcatcct tatgaggaac ctgcttatga tcttttcgct atagatgaac cagtcgaaat
    23881 gtttgggtta ggacgagtag gtgaattgcc gcaagaaatt tctatagagg catttgtaga
    23941 acaagtaaaa gaagcgttcc aactagatgg actgagaata gtccagccaa aaaatgcaaa
    24001 atcctctgtg aagcgcattg cgatttgcgg tggaagcggt gaaaaatttt atccgcaagc
    24061 aatcgcccaa agagcagatg tctatatcac gggagatatc tattatcata cggctcatga
    24121 tatgcaaagc gctgggttga ttgcaattga tcccggtcat tatattgaat cgttatgcaa
    24181 gcaacgattt atcgagaaat ttgaatcttg gaaacaagaa gaaaattggg atattaactt
    24241 tttcgtttct gaaacaaata caaacccatt tcaatttaat taaaaggaga gaatactatg
    24301 tacgaaaatc ttttgccacg ttttttacga tatgtcaaaa cagaaacacg ctccgatgca
    24361 acaagcaaaa cgactccatc gactcagaca caagttgctt ttgcgcaaga attgaaaaaa
    24421 gaattagaag agttaggctt agctgatgtt ttctataatg aagataacgg attcgttatg
    24481 ggaacattac caagcaatgt tgatcatgaa gtgcgttcca ttggatttat ttcccacatg
    24541 gatacagcag attttaatgc agtaggagtc aatccacaaa tcattgaaaa ctatgatggc
    24601 gagtctacga tcgtactaga tgcggaagaa cgctatacat tgaatacaaa agattttcca
    24661 aatttgaaaa attatgaagg ccacacattg atcacaacag atggcagtac attactggga
    24721 gcagatgaca aatcagggat tgcggaaatc atgactgcaa tggagatctt gatcaataat
    24781 ccttctatcc ctcacggtac catcaaagtg gcttttggtc ctgatgaaga aatcggtgtc
    24841 ggagcggata aattcgatgt tgagcaattc gatgtagatt ttgcttatac agtcgatggt
    24901 ggtcctttag gtgagttgca gtatgaaacg tttagtgctg ctcaagcaaa tattacgatc
    24961 caagggaaaa atgtacatcc agggacggca aaagatacaa tgatcaatgc tctacagtta
    25021 gcaatcgatt ttcataatca gcttccagcc gatgaagttc cagaaaaaac ggaaggatac
    25081 gaaggtttct tccatttgat ggcattggat gggaatcctg aagaagcttc tatgagttat
    25141 atcattcgcg atcatgatcg tgaaaaattc gaagcacgca aagcacaaat cacacatatc
    25201 caagaagagt tgaatcagcg atttgatcaa gaacgaatca aagtagatat gtatgatcaa
    25261 tattacaaca tgaaagaaat cattgaaaaa gatatgagca tcgtggaatt agcgaaacaa
    25321 gcaatgatcg agcttggtat ctctccagtg atcgaaccag ttcgtggagg aacagatggt
    25381 tcaaaaatct cttacatggg tattccgacg ccaaatctat ttgccggagg agaaaatatg
    25441 cacggacgat tcgaatttgt ttctttacaa gtaatggaaa aagcaacaga cgtgattgta
    25501 aaaattgcag aattgaacac aaagagttaa aatcgaaaaa gaacagctta tgacaaggcg
    25561 ttcgtcccaa tgatggaatg tcatctgtct aaggctaaag ggcaggacaa tggaattggt
    25621 tgtcccgccc tcttttttga agagtaaata ggaggtaaag aatggaaaag ctgctagttt
    25681 tttataacaa aagttcaggg aaagatgagg gtgagaaatt agcacgctgg tttcaagatt
    25741 atgtaaaaga aaagcagcca gaattatatg tagaactagc tgaaactggc ccatcgattg
    25801 atgatgcgga attagtcaag aaagcagaag ccatagaagc tgatacttta gtaattatag
    25861 gtggagacgg cacgattcat catatcgtcc aagcgttcca aaaaacatta tctcaatacc
    25921 atgtcggatt acttccaggt ggaactgtca acaaccttgc acgtgttctt gagattcccc
    25981 tcaaaaaaga agaagctgct gacgtgatcc tggaaggcaa tgtacggaaa atagactatg
    26041 gtcaagtcaa tgatgaagtc attatctcta cactgacaat cggaatctta gcagatacag
    26101 cagtttggat cagtcaagaa gaaaaacagc gttacggctc gtggatcttt atcaagaagt
    26161 tttttaagtt attgctgaag aagaaaaaat atcatttgga tttagaaaca gacgaggagc
    26221 attggaaagg aaaatcccaa ctgttgacag tcacgatgtc taactccgtg ggcggtttta
    26281 caaattttga cgactcagcg accctagatg atggaaaatt tcatgtaacg atcgtacctt
    26341 ccttagatat tttccgattt attttttatt tgcctagaat catgagaggc aaattctata
    26401 cgatccccgg cattacttat ttcacagcca aacgcatgaa aatccaagcg caggaaaaat
    26461 ctgtgggcac tcgaacggat ggagacacga cagatgatct tccagttgaa ttatctgttg
    26521 tatcaaaagg attaacagtt tattcaccaa agaaagaaga aaaagcttag aaatagtagt
    26581 taacagaaag ttggaagaag agtaatggat gtattgttac acaatccttt gtggttttac
    26641 cttttcattg tgaattttta cttattttgt ctgatggggt atgataaaca tcaggctaga
    26701 actagcggct ggcgtgttcc tgagaataat ttgatatttg tcagcctgtt aggcggtggt
    26761 ttgggaggat tgatctctcg aaaagttttc catcataaaa ctcggaaaaa gaagtttact
    26821 ataggattcg ctgcaggtgt acttgttgat ttgattctca tttatttttt ccattaagga
    26881 gcaggagatg tcgtctaaag gaacgaaaac taaaatcatt ctgaatctgc ttttgctgat
    26941 tgtgattttt gccattattt attatttaat caatcaatca tttgcagata tctttgccga
    27001 attgctgtct accagtgttg ttgttttggc agcggctgtt atattaggga cagtttacca
    27061 aatcgctgaa gggcgctcaa tcaaagaaat tgctgaacct ttcgcgccga attttacaac
    27121 gaaagatggg ttcttgacct ctagctatat tgcgttttac cgaatcgttt cgtttggtac
    27181 aggaacgctg atctcggaga tttattttta taacaaaaaa ggaatgaagc tatcccaggg
    27241 agcaggggtg acagcactcc atatgattat gtacaaactg gcagtgatca cttatgcatt
    27301 agctggcttg gtcatccaat tctctctttt ttattctaat ggtccatcaa tgatctggtt
    27361 tatcatcgct ggtatccttt tgacgatcgt gattgttgcc tttttattgg cgatgtccgt
    27421 cagcttgaat gttcaaatat tttttgtcag cttatcgaat aagttattta aaagtgagcg
    27481 tttgcggaaa tgggtggata catgcaatca gcagatctac tctttaaggg aaacggtcca
    27541 aaccattatc caagacagga ctgcgctatt gcggatttat ctatggaata tgttaaaact
    27601 tgtattttgg tatgtacttc cttatttgat tctgattgaa aaccatccta aaatagattt
    27661 tctactgacc atttcgttta tcagctttgc agtagtgctt tcaggggtta ttcctacgcc
    27721 tgctggtatt ggttcctttg aatttgtcta tttgttatta ttcagaccag ttgttggaac
    27781 agttgacgca gtttcttcgc ttttactgta tcgtttcagt tcctttatct tgccatttct
    27841 ctatggattt gtgtatgtga ttgctgaacg gcgaagagtg atcaaagaag aaataataga
    27901 agtaaaaaaa gagaataaag aaaatcaagg gtaggtcttt tataagatct acccttttta
    27961 tcaaatcaaa gaaaaacgtt atggataaga taattttacg tttgtttg
//
LOCUS       DARBET010000030        26161 bp    DNA     linear   BCT 07-NOV-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4027
            SAMEA114569841-rid20112183.denovo.030, whole genome shotgun
            sequence.
ACCESSION   DARBET010000030 DARBET010000000
VERSION     DARBET010000030.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA114569841
            Sequence Read Archive: ERR12203666
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 26161)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 26161)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001972618.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001972618.1
            Genome Coverage       :: 132x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/07/2023 13:18:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,041
            CDSs (total)                      :: 2,975
            Genes (coding)                    :: 2,908
            CDSs (with protein)               :: 2,908
            Genes (RNA)                       :: 66
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 67
            CDSs (without protein)            :: 67
            Pseudo Genes (ambiguous residues) :: 0 of 67
            Pseudo Genes (frameshifted)       :: 26 of 67
            Pseudo Genes (incomplete)         :: 28 of 67
            Pseudo Genes (internal stop)      :: 20 of 67
            Pseudo Genes (multiple problems)  :: 6 of 67
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..26161
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA114569841-rid20112183.denovo.030"
                     /isolate="VRE4027"
                     /db_xref="taxon:1352"
                     /country="Denmark"
     gene            <1..339
                     /locus_tag="SCJ86_001462"
     CDS             <1..339
                     /locus_tag="SCJ86_001462"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287843.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HEG1169007.1"
                     /translation="QASSKEEANNQIRFMIDKWKKQYPRVVKLLMNPAILTFYNFPPS
                     IRRTIYSTNLIEGFNKQLKKYTKRKEQFPNEESLERFLVSQFNNYNQKFLCRVHKGFK
                     EIHDTLESMF"
     gene            812..1366
                     /locus_tag="SCJ86_001463"
     CDS             812..1366
                     /locus_tag="SCJ86_001463"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318477.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HEG1169008.1"
                     /translation="MSYNVQPLRTNEEIQDFQFWLRRTSNPERDSFLFLFGINNGLRM
                     SDIIGLKVGDIRGKSKPIIVEHKTGKRKPIFIDNLREEILLYTEGKEENDWLFPSRQQ
                     GRHITRDRVYQIYADIAEKLGRDDIGTHTLRKTFGYHYYKKTRDIATLMFIFNHSSQA
                     ITKRYIGITEDEIGASLRGFKLGV"
     gene            complement(1539..2012)
                     /locus_tag="SCJ86_001464"
     CDS             complement(1539..2012)
                     /locus_tag="SCJ86_001464"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HEG1169009.1"
                     /translation="MRIKPIKDGEQLLFSSEENKPLYLDYVNHNLKIIIKENNLKHIT
                     LHGFRHTHCSLLFESGASVKEVYVRLGHTDIKTTMDDIYTHIAKKQTEETAIRFADFM
                     SSTEEKQGSLQKVKSMVKNEKSRLSLLDSLLNVELSVFFLFRSVCVFETVLILFF"
     gene            2024..2560
                     /locus_tag="SCJ86_001465"
     CDS             2024..2560
                     /locus_tag="SCJ86_001465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342357.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HEG1169010.1"
                     /translation="MPARIHHISIVNRFIRPTFDFYHNILGLKLLMKTINQDDHTMYH
                     LFFSDNHHRVGTELTFFEVQEGNNQFFGTNTIERTILKVPSEASLHFWEIYFETQGVC
                     HYGIESFSGRPILRFEGPDATRLALVPLREFEDIDDYFPYVTDQIPTEHAILGIDAIQ
                     LRVQYSDATERELLSVGW"
     gene            2600..2971
                     /locus_tag="SCJ86_001466"
     CDS             2600..2971
                     /locus_tag="SCJ86_001466"
                     /inference="COORDINATES: protein motif:HMM:NF013098.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HEG1169011.1"
                     /translation="MTILENQDTQFYQEVQIIQDRENPVAIEGIGSVHHVAFGVENKS
                     DLQRIDKQLQERNFINSGIKDREFFVSLYYRDPNQLLIEIATGEGNLDAKAYENQSPR
                     FEEIPLFLPQYLNFIREQVEQ"
     gene            3255..4526
                     /locus_tag="SCJ86_001467"
     CDS             3255..4526
                     /locus_tag="SCJ86_001467"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324539.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="substrate-binding domain-containing protein"
                     /protein_id="HEG1169012.1"
                     /translation="MKLSTQEKQLKNVSLKIPKNKISIFTSVSGSGKSSIVFDTITQE
                     AGRQLNDTYDSFTRLFLPKYSRPEVDEIDNLSTAIVVNQKKIGGSARSTLGTITNINA
                     LFRVLFSCFATPSLGYANAYSFNDPSGMCSVCQGIGKTIAFLIGLLYFSAIDNADFVF
                     SDPFTAEVLSGIEEQAKNHGYFVLVHNVNSAEDVKTIQRNWRFEGFIVVGVRVKEFLE
                     LNNVFDGPVVYIDTHLSKETLRELEQQMNRLFVNTNDYQASTLAVEHLIHYGHEKIAF
                     LSFDYIVGEPSVIQERHQAYIDTLKKYHLTFKSHWIYNNSEFERIYQELAEFTAIVVT
                     GDYLAAKLVKFLKNKNKRVMEQLSIIGFDDITFAELMDPALTTIRLNPGNKGAIAFHQ
                     LIGLAKQEEIKNQVLLLEGELISRDSVHKIK"
     gene            4771..5796
                     /locus_tag="SCJ86_001468"
     CDS             4771..5796
                     /locus_tag="SCJ86_001468"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290378.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HEG1169013.1"
                     /translation="MVGIRDVAKRAGVSIATVSRVLNEDPTLSVTDETKRKIAESVNF
                     YHYVKKSPVAKNLTNIALITTVSEVDELEDPYFRTIRRGIQVEAEQSKVNLKKIIRLS
                     ETPLEQDDLLKCQGVLIIGQVLSSVIEEVSAINSNVVVIDDPSIEAEVDAVYTDLTKA
                     TNQHLDRLYKKGHRNIAFIGGGRVRLDSQGNKHVSDDDYRKIAYETWMKQKGLEKYSQ
                     IYLEGWTTLDGMQAADLLLKKHAKDKLPTAIMVGNDPIAVGVYRSLQKNGLHIPEDIS
                     IVSFDNIEVAEFLTPALNTVNVNTEEIGKLAVRMVNERIKEVRNVSIKVIVSNNIIVR
                     ESEKNID"
     gene            6090..7199
                     /locus_tag="SCJ86_001469"
     CDS             6090..7199
                     /locus_tag="SCJ86_001469"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290375.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter substrate-binding protein"
                     /protein_id="HEG1169014.1"
                     /translation="MLDEFEKQNPDIKVEMETTPWDQYWVKLEAATTGGNMPDVVTMH
                     SSESYKYMSQNALMGLDDVIKENDIDMGNFTEGIADFYTLEDELYAIPKDASVVGLWY
                     NKELFDNAGVDYPDETWTWETLRQAAIDLTDESKGIYGFASHIGTETGYWPFIYQNGG
                     EVFADDNTKSGLNTPEAQEALAFYRDIALVDHASPTITELQDPDKLSRFQSGRLAMMI
                     EGNWFAPGFLKNDYLVENVASAVLPKGKVNATITNGLGWAASASTKYPEEVKKLMAFL
                     ASEDANKIQAETGASIPALKGFEDEWAASSDTIDLSAFGKMLEYGVPRPYNKDGLRAE
                     QAEVQIINRILSGEVTVEDGTAQIEKEVNAILSGN"
     gene            7282..8178
                     /locus_tag="SCJ86_001470"
                     /pseudo
     CDS             7282..8178
                     /locus_tag="SCJ86_001470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017648409.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
     gene            8194..9018
                     /locus_tag="SCJ86_001471"
     CDS             8194..9018
                     /locus_tag="SCJ86_001471"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002405323.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HEG1169015.1"
                     /translation="MKNKFFSNAPIHIMLILGGIAMALPFIWMILTSGKTLVESTQIP
                     PTIFPEKFQLSNYQQVLDLLPFMAFYINTFLMVVGRVIGSVLFSAMAAYACARLSFPG
                     KNILFSLVLIQMMIPGSIFITPQYLLAQKLGILNTIPALILPGVVSAFGTFLLRQFFL
                     GLPDELEEAAYLEGANIWTIFFKIMLPLAKSGLVALGIFTALFAFKDLMWPLIVNTSI
                     DKMPLAPGLANLQGQYATNFPQLMAGSLLAIWPIVVIFIVFQRKFIEGIATTGSKL"
     gene            9050..10360
                     /locus_tag="SCJ86_001472"
     CDS             9050..10360
                     /locus_tag="SCJ86_001472"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005235059.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-glucosidase/alpha-galactosidase"
                     /protein_id="HEG1169016.1"
                     /translation="MTKITFIGAGSTIFAKNVLGDAMLTPSLQDAHIALYDIDEKRLK
                     ESNLMLKNINKNSNENRADIVSYYDRKEALREADFVINAIQVGGYKPSTVIDFDIPKK
                     YGLRQTIGDTMGIGGIFRTLRTLPVMLDFAKEMEEVCPNAWLLNYTNPMAMLTMGVLK
                     ATSIKTVGLCHSVQVCVPELFEHLGIADQYDLNDFQWKIAGINHMAFLLEITKNGEDF
                     YPTIRQLAAEKPNPHPDSVRFELLKRFGYYITESSEHNAEYHPYFIKSKYPELIDELG
                     IPLDEYLRRCEQQINDWESMREDIVNNGELTHTRSREYASYIMDSITTGTPSVIAGNV
                     LNKGLITNLPTDVCVEVPCLVDKNGVQPTYVGELPTQLAALNRTNINVQELTVEAALT
                     LDKSKIYQAAYLDPHLSSELSLTDIKNLVDDLIEAHGDYLPEYN"
     gene            10508..11767
                     /locus_tag="SCJ86_001473"
     CDS             10508..11767
                     /locus_tag="SCJ86_001473"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343070.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HEG1169017.1"
                     /translation="MKWSKKIILACIALGATISLTACGKNQEDASGKVEIEFFNQKKE
                     MTQTIQEIAKDFEAKNPDIHVKVVDVPNAGEVIKTRMLAGDVPDVINLYPQSIELKEW
                     AKAGYLEDLTEEPYLENIKNGYAQRFAIEDRVYSIPLTANVYGFYYNKTAFEEMGIQA
                     PETWAEFEKIVAEIKDQNKVPFAIAGAEGWTLNGYHQLAIATAAGGEEEANNVWRFSE
                     VNGINAESKEMQKDFNRLDLLREPKALQNNWQGAGYNDTVVTFTKGDALIMPNGSWAM
                     PMIHSQNPDFEVGTFPFPADEAGQSLTIGAGDLALSVSATSQHKEEAKRFVEYMTTPE
                     AMQKYYDVDGAPCAVEGVIENTQDSPLSGLTELAFTDRHLVWLAKDWNSENDFYTLTT
                     NYLHNGNQKAMINALNAFFNPMKADGR"
     gene            11777..12649
                     /locus_tag="SCJ86_001474"
     CDS             11777..12649
                     /locus_tag="SCJ86_001474"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018363968.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HEG1169018.1"
                     /translation="MKIKEILNRYWAIIFVAIPIILQMIFFYFPMVQGAFYSLTNWTG
                     LTYNFDFVGLNNYKILFSDPKFIKSIGFTLIVTLCMIAGQIGFGILIARALNANIKGK
                     TFFRAWFFFPAVLSGVVVSLVFKQIFNYGLPKIGSMLGIEFLQTSLLGTTWGAIFAVI
                     FVLLWQGIAMPVIIFLAGFQSIPQEIIEAARMDGANDKQIFWNIEITYLLPSISMVFI
                     MALKGGLTAFDQIFALTGGGPSDATTSLGLLVYNYAFKNNQFGYANAIALVLFILIGI
                     VSLAQLKLSKKFEI"
     gene            12661..13491
                     /locus_tag="SCJ86_001475"
     CDS             12661..13491
                     /locus_tag="SCJ86_001475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000711263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HEG1169019.1"
                     /translation="MKTSKEKNISKYLLLLLGGFMILVPLVSTLFGSFKTTPDIMNNF
                     FRFPNPATLENFRRLLADGIGHYFWNSAIITIIAVVAVTAIIPMAAYSLARKMTRGKA
                     YGLMYLFLILGIFVPFQVIMIPITVMMSKIGLANMWGLILLYLTYAVPQTLFLYVGFI
                     KLNVPISLDEAAEIDGASHFTTYFKIVFPMLKPMHATTIILNALWFWNDFMLPLLILN
                     RDSNMWTLPLFQYNYTGQYFNDYGPSFASYTVGIVTITLVYLAFQRSIIAGMSNGAVK
                     "
     gene            13531..15714
                     /locus_tag="SCJ86_001476"
     CDS             13531..15714
                     /locus_tag="SCJ86_001476"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-galactosidase"
                     /protein_id="HEG1169020.1"
                     /translation="MIIFDNHTQTFHLQNKKISYLLGIEENNYLTHLYFGKKITHYSG
                     GYRYPRTDRSFSPNNANAQDRLLSLDTLPLEFPGYGHGDFREPAHNIKLGNGSRVNDF
                     RYDSYEIQKGKPSLAGLPALYTEDDDEAETLIIQLKDAISGLTLQLFYTIYQNHSVII
                     RSTQLVNNSDETVEINRLASQSIDFPNRPLELIHLNGTWARERQMTREKIHTGIKVLD
                     SKRGSSSHQQNPFVALVDAKTDEFQGEAYGFNLVYSGNHETVIQKDPFDQTRVIMGIN
                     SFNFGWQLKTGDSFQTPEVVMVYSNEGLNTLSQTYHELYNHRLIRGNYRLKERPTLIN
                     NWEATYFDFNEEKIMNIVDDAKNLGIEMFVLDDGWFGGRENDFTSLGDWVETEGKLAC
                     GLENLAKQVHDNGMKFGIWFEPEMISEDSELFRAHPDWALGIPGRDRSISRNQYVLDF
                     SRQDVRDNIYQQMTAIFDRVPIDYVKWDMNRNMTEVYSVLLDSTMQGEVAHRYMLGLY
                     EFLEKLTTNYPTILFESCSGGGGRFDAGFLYYMPQTWTSDNTDAVARLKIQYGTSLVY
                     PISSMGAHVSAVPNHQTGRETSLTIRGNAAMAGVFGYELDLTQLTSEEKAEIKEQVAF
                     YKKHRQLLQYGTFYRLESPFEGNDTAWMFVSKDQTEAMVFYFRVLAEAAHPLVTLKLA
                     GLAEEATYQMEDVTFAGDELMNLGFYIDSELHGDYATQRFYLKQI"
     gene            15775..16995
                     /locus_tag="SCJ86_001477"
     CDS             15775..16995
                     /locus_tag="SCJ86_001477"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oligo-1,6-glucosidase"
                     /protein_id="HEG1169021.1"
                     /translation="MFYLHAFTKKQPDLNWENEEVREEIYAMINYWLDKGLGGFRIDA
                     ILNIKKKLEYGIFESDGEDGLAFIGPWILNQSGIEVWLKEMRERTFKPHNSMTVAEAD
                     VPNERLDEYIGEDGYYSMVFDFSYTDIDVPETGEWFKDSQWTWTDMRTNIFTNQLVTQ
                     DKGWGALYLENHDQPRSINKYIPEEWINDYSKKMLATLFMLLRGTPFIYQGQELGMTN
                     IEMASLEDFDDVATHSQYKRALEYGLAPEQALKAVNKRSRDNSRTPMQWNTQKNARFS
                     TSDNVWLKVNPNYPELNAEAQLTNQQSVFNYYHQLIKLRKNSVYKDVLIYGQFLPVKD
                     KNEHILLYERQLEDKIVLVILNVTATEQEYYVDEQYQQVLINNYEDVLLDQSRKLRLR
                     PFESIVLANYGGQL"
     gene            16992..18452
                     /gene="gtfA"
                     /locus_tag="SCJ86_001478"
     CDS             16992..18452
                     /gene="gtfA"
                     /locus_tag="SCJ86_001478"
                     /EC_number="2.4.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290364.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sucrose phosphorylase"
                     /protein_id="HEG1169022.1"
                     /translation="MKIKNEAMLITYADSLGKNMGELKQVLDKHLQGVIGGVHLLPFF
                     PSTGDRGFAPSDYTTVDPALGTWEDVEALGEDYYLMFDFMINHISRESKFFQDFKKNH
                     EQSPYKEMFIRIHEFFPENRPTQEDIDLIYKRKDKAPFQTVEFADGTTEEVWNTFGEE
                     QIDLDVTKEVVKEFIRETIKDMANHGCSLIRLDAFAYAIKKLDTNDFFVEPDIWELLD
                     EVRQEAAKYDVELLPEIHEHYSIQMKIANHDYYIYDFALPMVTLYSLYSGKSERLANW
                     LKMSPMKQFTTLDTHDGIGVVDARDLLTDEELDYTSEELYKVGANVKKVYSSANYNNL
                     DIYQINSTYYSALGDNDRSYLLARAIQVFAPGIPQIYYVGLLAGKNDIELLEATKEGR
                     NINRHYYDLAEIEEEVQRPVIQQLFALLKFRNESAAFDLDGTIEVATPSESEIVITRK
                     NKNGSDSATLTANLDTKEFIIESKDIKIFTSVALKF"
     gene            18529..19635
                     /locus_tag="SCJ86_001479"
                     /pseudo
     CDS             18529..19635
                     /locus_tag="SCJ86_001479"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016175341.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS4 family transposase"
     gene            19957..20166
                     /locus_tag="SCJ86_001480"
     CDS             19957..20166
                     /locus_tag="SCJ86_001480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3173 domain-containing protein"
                     /protein_id="HEG1169023.1"
                     /translation="MVVITKNELMKLGYGKYQSERIIRQAKAIMVSKSYTYYENRRLG
                     LVPVESVEEVLGMKIEVEEDFQNHG"
     gene            20159..20308
                     /locus_tag="SCJ86_001481"
     CDS             20159..20308
                     /locus_tag="SCJ86_001481"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290356.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="integrase"
                     /protein_id="HEG1169024.1"
                     /translation="MVNFKKDKNGKYHFTASLGFDKRTGKRVQKNVEGLKQLKKKKSV
                     YRTCF"
     gene            20462..21127
                     /locus_tag="SCJ86_001482"
     CDS             20462..21127
                     /locus_tag="SCJ86_001482"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342363.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HEG1169025.1"
                     /translation="MELREIRPIDIQKWQNNLLSKYKNSYVRNMYGIFQMSLDRAVVL
                     GLLEKNPAKVIGNVKKRKTTVDFWTKEEFTKVISTFYIDDLYQHFGFICMWLLFMTVM
                     RLGEATALTWKDIDFENKNLRVNKTLYYKRAKTYEFVEPETASSIRTIELDDNTITFL
                     KAWKERQVSIGGMSFVLSYNGTPTQKYTIRHIMRRHATIVGVHIIRIHGLRHSHASLL
                     ISQ"
     gene            complement(21411..22496)
                     /gene="xerS"
                     /locus_tag="SCJ86_001483"
     CDS             complement(21411..22496)
                     /gene="xerS"
                     /locus_tag="SCJ86_001483"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177643.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine recombinase XerS"
                     /protein_id="HEG1169026.1"
                     /translation="MKNTEIYQKIDTIILHMPDYIKDFVQDREDKDQSPRTTLEYLKN
                     YKLFYEWLLSESIVPDTSITSIRQLTAYDLNLYKSHLKRRTKENVKKETTKTKLKDNN
                     NLGLSTSTINRNITALKVLFKYLSKSSNNPLGKPYLDNNPMDQVATITDKTTLAARAN
                     AIEKKLFLDKDTQNYLDYIANEYKNTLSKRALIYYQRDVERDLAINALILGSGLRLSE
                     VININLDDLSLDKNNVVVTRKGNKRDAVNIAAFAMEYLANYLAIRKERYKVTENEKAL
                     FLAIYQGEAKRISGIAIERMVAKYSKGFRIQVSPHKLRHTLATRLYQQTNSLVLTAQQ
                     LGHSSTNTTTLYTHIDNAATIDALNAL"
     gene            22830..23063
                     /locus_tag="SCJ86_001484"
     CDS             22830..23063
                     /locus_tag="SCJ86_001484"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290351.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="addiction module antitoxin"
                     /protein_id="HEG1169027.1"
                     /translation="MAIKERKFREVGNSVVLTISKEFLKENGLNPGDTVFLDEEKLRA
                     AITKKQALSDEIDLAISQALTEYDTTFRELVDK"
     gene            23060..23467
                     /locus_tag="SCJ86_001485"
     CDS             23060..23467
                     /locus_tag="SCJ86_001485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307501.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system death-on-curing
                     family toxin"
                     /protein_id="HEG1169028.1"
                     /translation="MSIHYLEAKDIIKMNVIQIKKYSPNEPLGVKDANALEMCVGQLR
                     ASAFGEEVYPSIYEKAAILLIQLIKKHPFHNANKRTAFLATFVFLKINGQLLAIDQKD
                     AVNLAVYIATYDKDFDQLKYEVINTIKTHTQPI"
     gene            complement(24604..25122)
                     /locus_tag="SCJ86_001486"
     CDS             complement(24604..25122)
                     /locus_tag="SCJ86_001486"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013852041.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ClbS/DfsB family four-helix bundle protein"
                     /protein_id="HEG1169029.1"
                     /translation="MRTYKNKEELKKEINRTYTKYILEFETIPEELKDKRVEEVDRTP
                     AENLAYQVGWTNLILKWENDERNGLSVKTPSDQFKWNQLGELYKWFTNTYAHLPLKEL
                     EEILNRNIDDINMMIDSMSDEDLFTAHKRKWADEATKTAVWEVYKFIHVNTVAPFGTF
                     RTKIRKWKRLAL"
BASE COUNT         8938 a         3987 c         4848 g         8388 t
ORIGIN      
        1 caagcaagtt caaaagaaga agcaaataat caaataaggt ttatgataga taaatggaaa
       61 aagcagtatc cacgagtggt taaattgctc atgaatcctg ctatattaac cttttataat
      121 ttccctccgt caatcagaag aactatttat tcaactaatt tgattgaagg ttttaacaaa
      181 caattaaaga aatataccaa aagaaaagag caatttccta atgaagaatc tctggagaga
      241 tttctagtat ctcaattcaa caactataat caaaagtttc tatgccgagt tcataaaggt
      301 tttaaagaaa tacatgatac attagaatca atgttttaac ttgatcaaca gaatgggctt
      361 ttccatttac acaaaattat tgacgctccc gacaaaatta tgatcttgcg taaagttata
      421 aaatattaat aaaaatattt tattaatgaa aagcttggta gcacggttat tcatatgcta
      481 atttatttca ctttatagta aggggagttc ttagtatatt gttcaaactt aaatttcata
      541 taatttaatt atgtaaatta gctattctaa gaacttctac ggataactgc ccgtctaaaa
      601 tttaaataag agcttagaat cttcatataa gtaaggttaa tttttttgta caaaaaagtc
      661 ggaatattct gagttactga aatgatataa aatcataaac taaatccctt gattttattg
      721 actttcaaaa gtttatatat ataagttaat gattatggta aaatatatac ataatcatta
      781 actcagaata tttttaagaa gggaaacaat agtgagctat aatgtacaac cgttaaggac
      841 aaatgaagag atacaagatt ttcagttttg gctaagaaga acaagtaatc cggaaagaga
      901 tagtttttta tttttgtttg gaatcaataa tgggttacgt atgtcagata ttataggatt
      961 aaaagttgga gatatacgag gcaaaagtaa gcctattatt gttgaacata aaacaggtaa
     1021 gcgaaaaccg atttttatag ataatttgag agaagaaatt ttattatata ctgaaggtaa
     1081 agaagaaaat gattggttat ttccgagtcg gcaacaaggt cgacatataa caagggatcg
     1141 tgtctatcag atatatgcag atatagcaga aaaattagga cgagatgata taggaacaca
     1201 tacattaaga aaaacttttg ggtatcacta ctacaagaaa actagagaca ttgctacctt
     1261 aatgtttatt tttaatcata gctcgcaagc aattacaaaa aggtacattg gtataactga
     1321 agatgaaata ggagcatcac ttcgtggatt taaactaggt gtataattta aaaattttta
     1381 taaacagtaa agacaaagat taaaagctat ttttatctag aacgatttta tttcataaag
     1441 ttcgcggaat aaaagctggg gttactggta cttggtatag gttcgtgtta tataaactaa
     1501 gtaattaaat aacattttta ggttggaaaa ggatgaaatt aaaaaaagag aatcaatacg
     1561 gtctcaaaaa cgcaaacgct gcggaataag aaaaatactg ataactcaac gtttagcaga
     1621 ctatctaata gggatagtct gcttttttcg tttttgacca tacttttgac cttctgcaaa
     1681 cttccttgct tttcctctgt agaactcatg aaatctgcaa aacgaattgc cgtttcctct
     1741 gtctgctttt tcgcgatatg ggtgtatata tcatccatag tggttttaat atctgtatgt
     1801 ccaagtcgaa catacacttc tttgacagac gcaccagatt caaagagcag actacaatgg
     1861 gtatgacgaa aaccatgaag tgtgatatgt tttaagttat tctctttaat gatgattttc
     1921 aaattgtgat ttacatagtc taaataaagg ggcttatttt cttctgatga aaacaaaagt
     1981 tgttcaccat cttttatagg tttgattctc atttttagat attatgcccg cacgtattca
     2041 tcacatttct attgtgaatc gctttattcg accgacattt gacttttatc ataatatact
     2101 cggtttaaaa ttactaatga aaacaattaa tcaagacgat cataccatgt atcacctgtt
     2161 tttttcagat aatcaccacc gtgtgggaac tgaactgact ttttttgaag tacaagaagg
     2221 gaacaaccag ttttttggaa ccaatacgat tgaacgaacg atactaaaag ttccttccga
     2281 agcatcattg catttttggg aaatatattt tgaaacacaa ggcgtttgcc attatggaat
     2341 cgaatcattt tctggacgac ctatcttacg ttttgaaggg ccagatgcaa cacgattagc
     2401 tttagttccc ttacgcgaat ttgaagatat tgatgattat ttcccgtatg tcacagacca
     2461 gatccctaca gagcatgcca ttttaggaat tgatgccatt caactacgcg tacaatattc
     2521 cgatgcgact gaacgagagt tattgtcggt aggctggtag cacaaagaat acgtcagttt
     2581 cttttcgggt catcaacgag tgacaatctt agaaaatcaa gacactcagt tctatcaaga
     2641 agttcagatt attcaagatc gtgagaatcc agttgccatt gaagggattg gtagcgtcca
     2701 tcatgttgcg tttggcgtag aaaataaatc tgatttacaa cgcatcgaca aacaattaca
     2761 agagcgcaat tttataaatt caggaataaa agatcgtgaa ttttttgtat cactttatta
     2821 tcgtgaccca aatcaattat tgattgaaat tgctacaggg gaaggaaacc tagatgcaaa
     2881 agcgtatgaa aatcaaagtc cacgctttga agaaattcct ttatttttac cgcagtactt
     2941 aaatttcatt cgtgaacaag tggagcaata attagctaat caaatccatg acgaagagta
     3001 agaggttgac tcataagtca aaaaataaag aaaaacgcat caaatccatt ctaaagaatg
     3061 ttgatttgat gcggttccta tttttataaa ctatcatttt cgactaataa aactacttat
     3121 gagtcagcct ctcttttttt cttgattttt atacctcttt ctaggtaaac taaacatctg
     3181 ccaaaaatac cgtgtctcat tgaaacattc tcaactattt caaaataaag gggagtacca
     3241 aatgaacgag tatattgaaa ttatccacgc aagagaaaca acttaaaaat gtcagtctca
     3301 aaattcccaa aaataaaatt agtattttta ccagcgtatc tggttcagga aaatcatcga
     3361 ttgtttttga tactattacc caagaagctg gtcgtcaact gaatgacacc tatgatagtt
     3421 ttacccgttt atttttacct aaatacagtc gtccagaggt tgatgagatt gataatcttt
     3481 ctacggcgat tgtggtcaat caaaagaaga ttggtggcag tgcgcgctca acgttaggga
     3541 cgattactaa catcaatgcg ttatttcgtg tgttgttttc atgttttgca acacctagtt
     3601 taggatatgc aaatgcttat tcatttaatg atcctagtgg aatgtgttct gtctgtcagg
     3661 gaatcggtaa gacgattgca tttttaatag gattgcttta tttttcggct attgataatg
     3721 ctgattttgt attttctgat ccatttaccg cagaagtttt gtctggaatt gaggaacagg
     3781 caaagaatca tggttatttt gtattggtgc ataatgttaa ttcagcagaa gatgttaaaa
     3841 ctattcagag aaattggcgt tttgaggggt tcattgttgt tggtgtacgt gtaaaagaat
     3901 ttttggaatt aaataacgtt tttgatggtc ctgttgttta tattgatacg catctttcaa
     3961 aagagacatt aagagaactt gaacaacaaa tgaatcgttt atttgtaaat acaaatgatt
     4021 accaagctag tacattggca gtggaacatt tgattcatta tggtcatgag aaaatagcgt
     4081 ttctttcttt tgactacatt gtaggtgaac caagtgttat tcaagaacga catcaagctt
     4141 atatcgatac attaaagaaa tatcatttaa cctttaaatc gcattggata tataacaact
     4201 ctgaatttga gcgaatttat caagaattag ctgaatttac tgcaattgtt gttactggag
     4261 attatttagc ggctaaatta gtaaaatttt taaaaaataa gaataaacga gtgatggaac
     4321 aattatcgat tattggattt gatgatatta cttttgcaga attaatggat cctgcgttga
     4381 caacaatacg actaaatcca ggtaacaagg gggcgattgc ttttcaccaa ctgattggac
     4441 tggccaaaca agaagaaata aaaaatcaag tactactatt agagggagaa ttgatttctc
     4501 gagactctgt tcacaaaatc aaataaaaaa gctctgtgaa ataagtatag ttaacaataa
     4561 aatatccgaa cgatagagag aatgaactgt gatctagcag ttcctcgtcg caaattcact
     4621 agaattagca ttatttactc taaattgttc ggatattttt tcttgttttt aattatgatt
     4681 acagtgccta tttttaacga aatttgctta ttttcaaaat gtatgattat atgttagtaa
     4741 acactttcaa atatgaaaag aggagctatt atggtaggaa ttagggatgt tgcaaaacgg
     4801 gcgggtgtgt ccattgcaac agtatcgcgc gtgctaaatg aggatccgac actttcagta
     4861 accgatgaaa ccaaacgaaa gattgcagaa tcggttaatt tttatcatta tgtcaaaaaa
     4921 agtcccgttg caaaaaattt aacaaatatt gcattaataa cgacagtttc tgaagtagat
     4981 gagttggaag atccctattt tcgaacgatt cgcagaggga ttcaagtcga agcagaacaa
     5041 agcaaagtca atttgaagaa aattattcgt ttgtcagaaa caccgttgga gcaagacgat
     5101 ttgctaaaat gtcaaggtgt gttgattatt gggcaggtgc tatcttctgt gattgaagaa
     5161 gtatcagcaa ttaattcgaa tgtcgtagtg attgatgacc ctagtattga agctgaagta
     5221 gatgcagttt atactgattt aacaaaagca acgaatcagc atttagatcg attgtataaa
     5281 aaaggacacc gaaacattgc gtttatcgga ggaggacgtg ttcgtttaga tagccaagga
     5341 aataaacacg tgtcagatga tgattatcga aaaatagcct atgagacgtg gatgaaacaa
     5401 aaaggattag aaaaatatag tcaaatttat ttagaaggct ggaccacact tgacggaatg
     5461 caggcagcag atttactttt gaaaaaacat gctaaagata agttgccgac tgctatcatg
     5521 gtaggaaatg atcctattgc tgtgggggtt tatcgttcac ttcaaaaaaa tggtctacat
     5581 attccagaag atatttcgat tgtgagtttt gataatattg aagttgctga atttttaaca
     5641 cctgcattaa atacggtgaa tgtgaataca gaagaaattg gaaaactagc agttcgcatg
     5701 gttaatgaac gaataaaaga agttagaaat gtttcgatca aagtgattgt ttctaataat
     5761 attattgtta gagaaagtga aaaaaatatc gattaaaaaa gtaaacatcc aaaaaagggt
     5821 gtttactttt ttgtttacta atatttttag taaaaaatct cgacaaaaca ttaagtaaac
     5881 agttatggtg ttgttaacaa aaagaaaacg cattcaaaac aggaggaatt aagatgaaac
     5941 atttcaaaaa aatagcttta acagggatag ctgttttaac tgggacatta ctcgcagcat
     6001 gtggtggggg agatagtaat tctaaagacg ggcaagtaac tttaagctat gcgagttgag
     6061 atactgacca agctccagga ctaagaaaaa tgttagatga atttgaaaaa caaaatcctg
     6121 atatcaaagt agaaatggag acaacgcctt gggatcaata ttgggttaaa ttagaagctg
     6181 caacaacggg aggtaatatg ccagatgtag taaccatgca ctctagcgaa tcctataaat
     6241 atatgtcaca gaatgctttg atgggacttg atgatgttat caaagaaaat gatattgaca
     6301 tgggaaactt tactgaagga attgctgatt tctatacgct tgaagacgaa ctatacgcaa
     6361 tccctaaaga tgcttctgta gtggggctat ggtacaacaa agaattattt gataatgcag
     6421 gtgtcgatta tccagatgaa acatggacat gggaaacact cagacaagct gcaattgatt
     6481 taacagatga atcaaaaggc atctatggtt ttgcatcaca tattggaaca gagactggct
     6541 actggccatt tatttatcaa aatgggggag aagtatttgc cgatgacaat acaaaatcag
     6601 gtttgaatac cccagaagca caagaagctt tagcttttta tcgagatatt gcacttgttg
     6661 atcatgcttc accaacaatt acagaattac aagatcctga taagttaagt cgttttcaat
     6721 caggtagatt agcaatgatg attgaaggaa actggtttgc accgggattt ttgaaaaatg
     6781 actatctagt tgaaaatgtt gcttctgccg tgttacctaa gggaaaagtt aatgcaacaa
     6841 ttacaaatgg acttggttgg gctgccagtg cgagtacaaa atacccagaa gaagtgaaaa
     6901 aactaatggc atttttagct agtgaggacg ccaataaaat ccaagcggaa acgggggcat
     6961 caatcccagc actaaaaggc tttgaagacg agtgggctgc ttcttcagat acaattgatt
     7021 tgagtgcttt tggaaaaatg ttggagtatg gagtccctcg tccatacaat aaagatggat
     7081 tacgagctga acaagcagaa gttcaaatta ttaaccgtat tctgtctggt gaagtaactg
     7141 ttgaagatgg aaccgctcaa attgaaaaag aagtcaatgc gatattgagc ggaaattaaa
     7201 ttagttaatt atacaatata caggcgaaag gattctattt cgtctgtata ttaatggatg
     7261 aataccaaaa agggggatat tatgaaaaat cgaatttttg gaaaatatcg taaagatcaa
     7321 gtgatatggg gctatatttt tatttttcca acggcattag gattaatttt attaaatata
     7381 tggccagcaa ttcaaacagc aattttaagt tttcaaaaaa cggtagggtt cggtgatact
     7441 gaatgggtcg gattaagaaa ttaccaaaaa atgtttgcag acggtgaagt ttttcagtct
     7501 ttattaaaca cattaattta tgcagtggtt tcgataccta gtattgttgt attatccttg
     7561 ttggtggcag ttttaatgaa tagaaaagtt aaaggattgt cgatttatcg gacaatttat
     7621 tttctaccag tcgttgctgc accatccgca gtcgcaatga tttggcgctg gatgttcaat
     7681 aatgattatg gactgattaa taacatgttg gctaaaatag gtctcgatgg accagcatgg
     7741 ttaacggatc catcgattgc gatttattct attatcattg taggagtttg gagtgctatt
     7801 ggttataaca tggtgttatt actagccggc ttataagaaa ttcctaaaga ttattatgaa
     7861 gcagcggaaa ttgatggtgc tagtccaatc cagcagtttt tcaacattac gttaccatta
     7921 gttacaccaa cattattttt tgtagttgta acaacagtta tcaatgcttt ccaagtattt
     7981 gatgttattt ttatgatgat tactcctaat agtaccgcca tgtttaaatc acaatcatta
     8041 gcctatttat tctataaaca ttcatttatt ttaaatgaca agggatatgg ttcggcaatc
     8101 gttatgttcc ttttagtgat tatattaatt attactgcaa ttcaaatgaa gttgcagaaa
     8161 aagtgggtaa actattaagg aggctatcaa attatgaaaa ataaattttt ttcaaatgcg
     8221 ccaatccata tcatgcttat tttaggtggt attgcaatgg cattaccttt tatttggatg
     8281 attttaactt cagggaaaac gttggttgaa tctacccaaa ttccacctac aattttccct
     8341 gagaaatttc agctaagtaa ctatcaacaa gtacttgatt tattaccatt catggctttt
     8401 tatatcaata catttttaat ggttgttgga cgagtgattg gctcggtttt attttcagca
     8461 atggctgcat atgcttgtgc gcgtctaagt tttccaggga aaaatatcct tttcagctta
     8521 gtacttattc aaatgatgat tcccggttcc atttttatta ctccacagta tttattagca
     8581 caaaaattgg gtattttaaa tacaattcca gcattaatat taccaggggt agttagtgcg
     8641 tttggtacgt ttttattaag acaatttttc ttaggtttac ctgatgaatt ggaggaagcg
     8701 gcctatttag aaggtgccaa tatttggacg atctttttca aaattatgtt gccgcttgct
     8761 aaatcaggat tggttgcact tggaatattc acagcgttgt ttgcatttaa agatttaatg
     8821 tggccattga ttgtcaatac ttcaattgat aagatgcctt tagctccggg attagcaaac
     8881 ttacaaggac aatatgccac gaacttccca caattaatgg caggttcgtt attagcaatt
     8941 tggccaatag ttgtgatatt catcgttttc caaagaaaat tcattgaagg aattgctaca
     9001 acaggtagta aattataaaa ctattaatta gtgattggag agtaagtaca tgacaaaaat
     9061 tacatttatt ggtgcaggaa gtactatttt tgcaaaaaac gtattaggcg atgcgatgtt
     9121 aactccaagt ctacaagatg cacatattgc attgtacgat attgatgaaa aacgactaaa
     9181 agaatcaaat ttaatgctta aaaatattaa taaaaattcg aatgaaaatc gtgctgatat
     9241 tgtttcttat tatgatcgca aagaagcgtt aagagaggct gattttgtta tcaatgctat
     9301 tcaagtgggt ggttataaac cttcaacagt gattgatttt gatattccta aaaaatatgg
     9361 gttgcgacaa actattggtg acaccatggg gattggtggg atttttagaa ccctacggac
     9421 actaccagta atgttagact ttgctaaaga aatggaagaa gtttgtccaa atgcttggtt
     9481 attgaactat acaaatccga tggcgatgtt aaccatgggt gttttaaaag cgacgagtat
     9541 taagactgtt ggtttatgtc atagcgttca agtatgcgta ccagaattgt ttgagcattt
     9601 aggaatagcc gatcaatatg acttaaatga ctttcaatgg aaaattgcag gcattaatca
     9661 tatggcattc ttgttggaaa ttacaaaaaa tggggaagat ttctatccaa caattcgtca
     9721 gttagctgca gaaaaaccaa atccacatcc agattctgta cgttttgaat tgttgaaacg
     9781 ttttggttac tatattacag aatcaagtga acacaatgca gaataccatc catactttat
     9841 taaatcaaaa tacccagaat tgattgacga attagggatt ccgttggatg aatacttacg
     9901 tcgttgtgaa caacaaatta atgattggga aagcatgcga gaggacattg taaataatgg
     9961 ggagttaaca catacgagaa gccgtgaata tgcttcatat atcatggatt ctattacgac
    10021 aggaactccg tcagtgattg caggaaatgt cttaaataaa ggcttaatca caaacttacc
    10081 gacagatgtt tgtgtggaag taccttgttt agtcgataaa aatggcgtgc aaccaaccta
    10141 tgtcggtgaa ttgccaacac aattagctgc tttaaatcgg acgaatatca atgttcaaga
    10201 actgacagta gaagccgctt taacactaga taaatcaaaa atttatcaag cagcgtattt
    10261 agatccacat ctatcatctg aattgtcttt aacagatatc aaaaatttag tggatgattt
    10321 aatcgaagca catggggact atttacctga atataattaa aaagaagctg aaagagtaag
    10381 gaattttcaa ttccttactc ttttcttagt taaatttagt aaaaatattg acaaaacact
    10441 tagtaaacag ttacattagt gttaacaaaa agaaaacgca ttcaaattgt ataaggagga
    10501 gcataaaatg aagtggtcta aaaaaattat tttagcttgt atagcactag gagcaacgat
    10561 ctctttgact gcttgtggaa aaaatcagga agatgcaagc ggtaaggtag aaatagagtt
    10621 ttttaatcaa aaaaaagaaa tgactcaaac gattcaggag attgctaaag attttgaagc
    10681 aaaaaaccct gatattcatg taaaagttgt ggatgtaccg aatgctggtg aagtaattaa
    10741 aacacgtatg cttgcaggcg atgtaccaga tgtcattaat ctttatcctc aaagtataga
    10801 attgaaagaa tgggcgaaag ctggttattt agaggactta accgaagaac cgtatttaga
    10861 gaatatcaaa aatggatatg cacaacgttt tgctattgag gatcgtgtat atagcatacc
    10921 attaaccgct aatgtctatg gtttttacta taacaaaact gcttttgagg aaatggggat
    10981 tcaagcacca gaaacttggg cagaatttga aaaaatagta gctgagatta aagatcaaaa
    11041 caaagtaccg tttgcgattg caggagcaga aggctggact ttaaatggtt atcaccaact
    11101 agctatagcg actgctgctg gtggtgaaga ggaagcgaac aatgtttggc gtttctcaga
    11161 agttaatgga attaatgctg aaagcaaaga aatgcaaaaa gattttaatc gattagattt
    11221 gttaagagaa ccaaaagctt tgcaaaacaa ctggcaaggt gccggttata acgatacagt
    11281 agttactttt acaaaaggtg atgcgttgat tatgccgaat ggttcatggg caatgccgat
    11341 gattcattca caaaatccag attttgaagt aggaactttt ccttttccag cagatgaagc
    11401 aggtcaaagt ctaacgattg gcgcaggaga cctagcctta tcagtttctg caacttctca
    11461 acataaagaa gaagccaaac gttttgtaga atatatgaca acacctgaag cgatgcagaa
    11521 atattatgat gtagatggtg caccgtgtgc tgttgagggc gttatcgaga atactcagga
    11581 ttcaccatta agtggtttaa ctgaacttgc ttttactgat cgtcatttag tttggttagc
    11641 caaagattgg aatagtgaaa atgattttta taccttaaca acaaattatt tacacaatgg
    11701 caatcaaaaa gccatgatta acgcattaaa tgctttcttt aatccaatga aagctgacgg
    11761 aaggtaggaa aataaaatga aaataaaaga aattttaaat cgttactggg caatcatttt
    11821 tgtagcaatt ccaattattt tgcagatgat ttttttctac tttccaatgg ttcaaggtgc
    11881 tttttacagt ttaactaatt ggactggtct gacttataat tttgattttg tgggtttgaa
    11941 caattataag attctttttt ctgatccgaa gtttatcaaa tcgattggct tcactttaat
    12001 tgtgacgctt tgcatgattg ctggacaaat tggttttggt attttgattg cgcgggcgtt
    12061 gaacgcaaat attaaaggga aaacattctt tagagcatgg ttcttttttc cagcggtctt
    12121 atcgggcgtg gttgtttctt tagtatttaa acaaattttt aactatggtt taccaaaaat
    12181 tggtagtatg ttaggcattg aatttttaca aaccagtctt ttaggaacaa catggggagc
    12241 gatttttgca gtaatttttg ttttactttg gcaaggaatt gccatgccag tcatcatttt
    12301 cttagcgggt tttcaaagta ttccacaaga aattattgaa gctgctagaa tggatggggc
    12361 gaatgataag caaatttttt ggaatattga aatcacctac ttattaccaa gtatttcaat
    12421 ggtatttatt atggcattaa aaggtggctt aacagcattt gatcaaattt ttgctttaac
    12481 tggtggtgga ccaagcgatg ccacaacatc tttggggtta cttgtttata attatgcttt
    12541 taaaaacaat cagtttggtt atgcgaatgc tattgcttta gtcttattta tcttaattgg
    12601 gattgtttca ctggcacaat taaaattatc gaaaaaattt gaaatctagg aggtttccca
    12661 atgaaaacat caaaagaaaa aaatatttct aaatacttgc ttttattatt agggggcttc
    12721 atgattttag ttcccttagt ttcaacgcta ttcggttcat ttaaaacaac acccgatatt
    12781 atgaataatt tctttcgttt tcctaatcca gcgactttgg aaaatttccg aagattgtta
    12841 gcggatggaa ttggtcatta cttttggaat tcggcaatca tcacaatcat tgcagtagtt
    12901 gctgtgacag caattattcc tatggctgct tattcactag caagaaaaat gactcgtggg
    12961 aaagcatacg gattgatgta tttattttta attttaggaa tttttgttcc tttccaagta
    13021 attatgattc cgattaccgt tatgatgagt aaaattggtt tagcaaatat gtggggatta
    13081 atcttactct atctcactta tgccgtacca cagacactat ttttatatgt tggttttatt
    13141 aaactaaatg tgcctatcag tctggatgag gctgctgaaa ttgatggggc tagtcacttt
    13201 acaacttatt tcaaaattgt tttcccaatg ttaaaaccaa tgcatgcgac gactattatt
    13261 cttaatgctc tatggttttg gaatgatttc atgctaccat tattaatttt aaatcgtgat
    13321 tctaatatgt ggacactacc attgtttcaa tataattata cgggacaata ttttaacgac
    13381 tatggtccaa gttttgcttc ctatacagta ggaattgtca cgattacact agtttatctt
    13441 gctttccaaa gaagtattat agctggaatg agcaatggag cagtgaaata accaataaat
    13501 tattctcatt ataaaggagt tcataaactt atgattatat ttgacaatca cacacaaaca
    13561 tttcatttac aaaataaaaa aattagctat ctattaggaa tcgaagaaaa taattattta
    13621 acgcatcttt attttggaaa aaagattacg cactattctg gtgggtatcg ctacccccga
    13681 acagatcgtt ccttctcacc caacaatgca aatgcgcaag atcgtttatt atctttagac
    13741 acattaccat tagaatttcc aggatatgga catggtgatt ttagagaacc ggcgcataat
    13801 attaaattag gaaacggtag tcgtgtaaat gattttcgtt atgatagcta cgaaattcaa
    13861 aaaggaaagc ccagcttagc gggattgcca gccttatata cggaagatga tgatgaagct
    13921 gaaacgctga ttattcaatt gaaagacgcg attagtggat tgactttaca gttgttctac
    13981 acaatttatc aaaatcacag tgtaattatt cgttctacgc aattagtaaa taatagtgat
    14041 gaaacagtgg agattaaccg cttagctagt caatcaattg attttccaaa tcgtccactc
    14101 gagttaattc atttaaatgg tacctgggca agagaacgtc aaatgactag agaaaaaatt
    14161 catacaggga taaaagtatt agatagtaaa cgcggatcaa gcagccatca acaaaatccg
    14221 tttgttgctt tagtagatgc caaaacagat gaatttcaag gagaggcata tggatttaat
    14281 ttagtttata gcggtaatca cgaaacggtg attcaaaaag atccatttga ccaaacacgg
    14341 gtcattatgg gaattaattc atttaatttt ggttggcaac taaaaactgg cgactcattt
    14401 caaacgccag aagtcgtgat ggtttatagt aatgaaggtc tgaatacgtt atcacagacg
    14461 tatcatgaat tgtataatca ccgtttaatt cgtggaaact atcgtttaaa agaacgtccc
    14521 acgttaatca acaactggga agcaacgtat tttgatttta acgaagaaaa aatcatgaat
    14581 attgtcgatg atgcgaagaa cttgggcatt gaaatgtttg ttttagatga tggttggttt
    14641 ggcggtcgtg aaaatgactt cacttcatta ggtgattggg ttgaaacaga aggcaaactt
    14701 gcttgtggtt tggaaaattt agccaagcaa gtgcatgata acgggatgaa atttgggatt
    14761 tggtttgaac cggaaatgat ttctgaagat agtgaactat ttcgagcaca tccagattgg
    14821 gcattgggta ttccaggtcg tgaccgctca attagtcgaa atcaatatgt tcttgatttt
    14881 tcaagacaag atgttcgtga taatatctat caacaaatga cggcgatttt tgatcgtgta
    14941 ccgattgatt atgtgaagtg ggatatgaat cgaaatatga cagaagtcta ttctgtcttg
    15001 ttggactcaa cgatgcaagg tgaagtggct catcgttata tgttagggtt atatgaattt
    15061 ttagaaaagt taacaacgaa ttatccaact attttatttg aaagttgttc gggtggaggc
    15121 ggtcgttttg atgcaggttt cttgtattac atgccacaaa cttggacgag cgacaatacc
    15181 gatgccgtgg ctcgtctgaa aattcaatat ggaaccagct tagtgtatcc aatttcaagt
    15241 atgggtgcgc atgtttcagc agtcccaaat catcaaacag gaagagaaac gagtttaact
    15301 attcgtggca atgctgcgat ggctggtgtt tttggttatg agctagatct aacgcaatta
    15361 acttctgaag aaaaagcaga aattaaagaa caagttgcct tttataaaaa gcatcgccaa
    15421 cttttgcaat atggaacttt ttatcgcttg gaaagtccgt ttgaaggcaa tgatactgca
    15481 tggatgtttg tctcaaaaga ccaaacagaa gccatggtct tttatttccg agtattagca
    15541 gaagctgcgc atccattagt cacattgaaa ttagcaggat tagcagaaga agcaacttat
    15601 caaatggaag atgtaacatt tgctggcgat gaattgatga atctcggttt ttatattgac
    15661 tcagaactac atggtgatta tgcgacacaa cgcttttatt tgaaacagat atagtagctt
    15721 gaatttacaa tataagtgca acaccaaaag agactcattc caaacgaaga aaatatgttt
    15781 tatttacacg catttactaa aaagcagcct gatttgaatt gggaaaatga agaagtacgt
    15841 gaagaaatct atgcaatgat taactattgg ttagataaag gactaggcgg atttagaatc
    15901 gatgcgatat taaacatcaa aaaaaaacta gagtatggca tctttgaatc tgatggagaa
    15961 gatgggttag ccttcattgg gccctggatt cttaatcagt cgggcattga agtgtggtta
    16021 aaagaaatga gagagcggac atttaaacca cataatagta tgacggtagc agaagccgat
    16081 gttcctaatg aacgtttaga tgaatatatt ggtgaagatg gctattattc gatggttttt
    16141 gattttagtt atacggatat tgatgtacct gaaactggtg aatggtttaa agatagccaa
    16201 tggacatgga cggatatgcg taccaatatt tttacgaatc aattagttac gcaagataaa
    16261 ggttggggcg ccttatattt agaaaaccac gatcaaccac gttcaattaa taaatatatt
    16321 ccagaagaat ggatcaatga ttatagtaaa aaaatgttgg cgacgctttt tatgttacta
    16381 agaggtaccc cttttattta tcaaggacaa gaactaggaa tgaccaacat agaaatggca
    16441 tcattagaag attttgatga tgtagctacg cattctcagt ataaacgggc attagaatat
    16501 ggattggcac cagagcaagc attgaaagct gtcaataaac gaagtcggga taattctcgc
    16561 acacctatgc aatggaatac gcaaaaaaat gccagattct caacttctga taatgtatgg
    16621 ttaaaagtaa atcctaatta tccagaactc aatgcagaag ctcagttaac caatcaacag
    16681 tcggtattca attattatca tcaattaatt aaattaagaa aaaatagtgt atacaaagac
    16741 gtgctaatct acggacaatt tctaccagtg aaagataaaa atgaacatat tttgctttat
    16801 gaacgccaat tagaagataa aatcgtatta gttatcttaa acgttacagc aacagaacaa
    16861 gaatattatg tagatgaaca gtaccaacaa gtattaatca ataattatga agatgtttta
    16921 ttagatcaaa gtcgaaaatt gcgtttacga ccgtttgaaa gtattgtatt agctaattat
    16981 ggaggacaat tatgaaaatt aaaaacgaag caatgctcat cacctatgca gatagtttag
    17041 gaaaaaacat gggcgaatta aaacaagtct tagacaaaca tttacaaggt gttattggtg
    17101 gtgtacatct gttgcctttt tttccatcga caggtgatcg aggctttgcg ccgagtgatt
    17161 atacgacagt tgatccagct cttggaacat gggaagatgt ggaagcatta ggtgaagatt
    17221 attatttgat gtttgatttt atgattaatc acatttctcg tgaatcaaaa tttttccaag
    17281 atttcaaaaa aaaccatgag cagtcaccat acaaagaaat gtttatccgt attcatgaat
    17341 ttttcccaga aaatcgacca acacaagaag atattgattt aatctacaag cgtaaagaca
    17401 aagcgccgtt tcaaacggta gaatttgcgg atggtacgac agaagaggtt tggaatacct
    17461 ttggggaaga acaaattgat ttagatgtga ccaaagaagt tgtgaaagaa tttattcgag
    17521 aaaccatcaa agatatggcg aaccatggct gttctttaat tcgattagat gcgtttgctt
    17581 atgcgattaa aaaattagat accaatgatt tcttcgtgga accagatatt tgggaacttt
    17641 tagacgaagt gcgtcaagaa gcagcgaaat atgacgtcga attattacca gaaatccatg
    17701 agcattattc cattcaaatg aaaattgcca atcatgatta ttatatctat gacttcgcct
    17761 tgccaatggt cactctgtat tcactttata gtggaaaatc agaacgttta gccaattggt
    17821 tgaaaatgag tccgatgaag cagtttacaa cattggatac gcatgatgga attggtgttg
    17881 tggacgctcg tgatttatta acagatgaag aattggatta cacctctgag gaattgtata
    17941 aagtcggggc gaatgtgaag aaggtctatt caagtgccaa ttacaataat ttagatattt
    18001 atcaaattaa cagcacctat tatagtgcat taggcgataa tgaccgtagc tatctgttag
    18061 cacgtgcgat tcaagtattt gcgccaggga ttccacaaat ttattacgtt gggttattag
    18121 caggaaaaaa tgatattgaa ctgttggaag caacgaaaga agggcgtaat attaatcgtc
    18181 attactatga tttagccgaa attgaagaag aagtgcaacg gccagtgatt caacaattat
    18241 ttgcactttt aaaatttaga aatgaatctg cggcgtttga tttggatgga acgattgaag
    18301 tagcaacgcc atcagaatca gaaatagtga ttacacgtaa aaataaaaac ggtagtgata
    18361 gtgcaacatt aacggctaac ttggatacga aagaatttat cattgagtct aaagatataa
    18421 aaattttcac tagtgttgca ttaaaatttt aacctattcc atagaacaaa aaatccttta
    18481 tactagcact tggatgactt atccaagacc aataaaaagg actcatacat ggataagtat
    18541 aaaacaaata cagcatttaa taaatggttt tctgccataa aattaggaaa actttctgtc
    18601 aactcccaaa aatgtatttt tgattacaat tcgtatcgta aaaaactgtc ttttgaagct
    18661 gtgctcaagc tctttctcta cgcagttaat gaagaaaaag aaagtttgcg tgatttgagt
    18721 acagctctta tcgaccgatc gctccaacta gaaaccgatg tgtcatcgat tagtcatacc
    18781 caattgtctc gagcatttaa tgcacttgaa cctaaagtgt tagaagaaat ttttcaacaa
    18841 ctgttagaaa aagttcaaca tcaaatcaaa ccaaccaagc gaaatcatct gtatctgatt
    18901 gattccagta cgttttcgct tagtttaaaa cgtcataagt gggcgacttt ccgtcaaaca
    18961 aaatcaggtg tcaagttaca cttaaactat tgttatatgg acgatactac gatgtatcca
    19021 actgatttta ccttaacaaa tgcccatgaa catgatctca atcaactacc agtattggtg
    19081 aatcaaccag aggcaactta cgtgtttgat cgtgggtatt aggactttga aaagatggat
    19141 cagatgcatt gggatggcta tttcttcgtc actcggatta aaaaaaacac caaagttcac
    19201 gtgatagata ctctggaaac atcgccagaa acagaaattc tacgagacga gctggttcgt
    19261 ttaggatcta aaacctatct aaccgccaat ttccgactag tcactgttca agataagaac
    19321 ggacgtgtgt ttcaatttat caccaatcga atggacgtct cttcaaaaga aatctctgat
    19381 atgtatcacg ctcgttggca aatcgaattg tttttcaagc acatcaaaca acacatgacc
    19441 attaaaacct tcttttctca aagtgaaaaa ggcgtgcaga atcaattgat tttaacgatg
    19501 atttccgctc tcctcacgtt tctcatcaaa ttggagacga aaacggaaaa atcagtgttc
    19561 caaatcaaac gattctttcg ttatttactg tttcagccct ttgaatgttg tctagaaaaa
    19621 ttaattccca catgagaaaa aagaaatttg gctcgttact tttggttttc agttgaataa
    19681 cagataaaaa attttctgga aaaagtcatc ctctgcgtca gcacagtttt ttttgactta
    19741 ttttcagagg aaatagtttt cagaatattc taaaatgttt tatgcaacac tagtgatatt
    19801 ttttagaatt agatttctaa tgtgcatttt ttgagtatta aggagcaagt atacaaagat
    19861 ttataaagct gattaaattt gaataaaaat atgcaggaac gctactattt ttagtagtgt
    19921 tcttttttat atttgaataa aaaatgtggt ggttaaatgg tggttataac aaaaaatgaa
    19981 ttaatgaaat taggttatgg gaagtatcag tctgagagaa tcatacgaca ggcaaaagca
    20041 attatggttt caaaaagcta tacctattac gaaaatagaa gattaggact agtacctgtt
    20101 gaatctgtag aagaagtttt aggtatgaag atagaggtag aggaggattt tcagaatcat
    20161 ggttaatttt aaaaaagata aaaatgggaa atatcatttt actgctagtt tgggattcga
    20221 caaacgaaca gggaaaagag tacaaaaaaa cgtagagggg ttaaaacagt taaagaagaa
    20281 aaaaagcgta taccgaactt gtttctaatt atggaaaaga agcagaaact tctcaaagta
    20341 ctatgttgtt tgaggagttt ttttataaat tattcctacc ttattatcaa gggaaagtag
    20401 aagaaagaac atataaaaat cggttatcta caatcaaaaa aacttttctt atttctttaa
    20461 aatggagtta agagaaatac gtccaattga cattcaaaag tggcaaaata atttattgag
    20521 caaatataaa aattcttatg taagaaacat gtatgggatt tttcagatgt cattagatcg
    20581 agcagttgtt ttgggactac ttgaaaaaaa tcctgcaaaa gttattggaa atgtgaagaa
    20641 aaggaaaact acagttgatt tttggacaaa agaagaattt acaaaagtta tttcaacttt
    20701 ttatattgat gatttgtacc aacattttgg ttttatttgt atgtggttac tttttatgac
    20761 cgttatgcgt ttgggagaag ccactgcatt aacttggaaa gatattgatt ttgaaaataa
    20821 aaacttgcgt gtgaataaaa cactctatta taagagagcc aaaacctatg agtttgtaga
    20881 acccgaaacg gcttctagta ttcgtacaat cgaactagac gacaatacaa ttaccttttt
    20941 aaaagcttgg aaggagcgtc aagtttcaat aggaggcatg tcgtttgtac tttcttataa
    21001 tggaacacct actcagaaat atacaatccg tcatattatg agaagacatg caacgatcgt
    21061 tggtgttcat ataatccgca tacacggatt aaggcatagt catgcttctt tgcttatatc
    21121 acaataaaaa gatgcacttc gaattaaaga aaggttaaaa catgctgatg tacaaacgac
    21181 tttaggaact tatggtcatt tgtatctaaa ttcattgaga gagataacaa atgatcccac
    21241 agattatatc caaactaatt tttctaaaat caaaccgaca cctatttacg ataataaaaa
    21301 ataaatgata aacaaaagta ggatcacacc aaaagaaaca cactagaaaa actataatac
    21361 aggcttttct agcgtgttgt ttcttatttc cgcctattta tacattatac tcataaagca
    21421 tttaatgcat cgatcgtagc cgcattgtct atatgggtat ataaagttgt tgtattggta
    21481 cttgaatgtc ccaattgttg ggcagttaat accaatgaat ttgtctgttg gtagagtcta
    21541 gtagctaatg tatgacgcag tttgtgaggg ctgacttgaa ttctaaagcc ttttgaatat
    21601 tttgctacca tccgttcgat tgcaataccg gatatacgct tggcttctcc ttggtaaatt
    21661 gctagaaata aagctttttc gttttctgtt actttatatc gttctttccg aatagctaga
    21721 taattagcta aatattccat tgcgaatgct gctatattga cagcatctct tttatttcct
    21781 tttctagtta ctaccacatt attcttatct aaagataggt catctaaatt aatatttata
    21841 acctcggaga gtctcaatcc agatcctaaa atcagtgcat tgatcgccag gtctctttct
    21901 acatctcgtt gataatagat caaagctcgt ttactcaaag tatttttata ttcattggca
    21961 atataatcta gataattttg tgtatcctta tctagaaata attttttctc aatggcattg
    22021 gcacgtgcag ctaacgtagt tttatctgtg atggttgcta cttgatccat aggattattg
    22081 tctaagtatg gttttcctag aggattattg gaacttttcg aaagatattt aaatagaacc
    22141 tttaatgccg tgatatttct attgatagta gaagtagata gacctaagtt attattatct
    22201 ttgagcttag tttttgttgt ctcttttttt acattttctt ttgtgcgacg tttcaaatga
    22261 gatttgtaca agtttaggtc ataggcagtt aactgtctta tcgaggtgat agaagtatca
    22321 ggaacgatag actcactcag aagccattca taaaagagct tataattttt tagatattct
    22381 aaagtggttc gaggtgattg atctttatcc tcacgatctt gaacaaaatc ttttatataa
    22441 tcgggcatat gaagtatgat tgtatctatt ttttgatata tttctgtatt tttcatgtca
    22501 ttcatccttt caataagtag gggagaagag tatctctata attggactat accaattata
    22561 gaatgttttg tcaattggta tgctctaaaa tttattaatt tgtttcaaaa aaagcatata
    22621 aacaggtata cgtttaataa tttaaaataa atggtataca taaataggga aggaaataga
    22681 agggagcaat ctaaattttt tgaattttct taattaatta tatagaaaga acaatctttc
    22741 tatagagtta gacatgagag ggactatgct tgatttaaaa tcaacaagcg tatatactgt
    22801 atatacaaag aaaaaggagg gagaagacta tggcaattaa agaaagaaaa tttagggaag
    22861 tggggaactc agtagtatta actatttcaa aggagtttct taaagaaaat gggttaaatc
    22921 ccggagatac ggttttttta gatgaagaaa aacttcgagc ggcaattaca aaaaaacaag
    22981 ccttatctga tgaaattgat ttagcaatat ctcaagcatt aactgaatat gatactactt
    23041 ttagagagct tgtcgataaa tgagtattca ttacctagaa gccaaagata ttataaaaat
    23101 gaatgtaatc caaataaaaa aatactctcc taatgaaccc cttggtgtaa aggatgcgaa
    23161 tgctttagaa atgtgtgtag ggcaacttcg agcctcagct tttggtgaag aggtttatcc
    23221 ttcgatttat gaaaaagcag ctatcctact tatacaattg ataaagaaac atccttttca
    23281 caatgcaaat aaacgtactg ctttcttagc tacttttgtc tttttgaaaa tcaatggaca
    23341 gcttttagct attgatcaaa aagatgctgt aaatttagcg gtatatattg ctacgtatga
    23401 taaggatttc gatcaactaa aatatgaagt gatcaacact ataaagacac atacgcagcc
    23461 catttaagga gttactgtat gtgtctttat agggttatta gctgctatag cgaaataatt
    23521 gaagtaaatt acaaatagct ataattcaaa tccaactata tagaaaacgt aaattttcta
    23581 tatagttgga ttaagataac gactatttat tttatcatat ttttactata ccctatactt
    23641 ttacttggta tatttatgaa taatcgcaaa tttaccccta tactactagt ttgtaagtta
    23701 gattaataaa aatactaaag ttatttgttc ttttattaaa tacacctaca acttataaat
    23761 tgatggtatt tagataacac gaaagaataa aaataacagc tgatagtaaa gtttgttgac
    23821 gattgctact caatcgaaag tgagttagtg cgtatgaact tgcttctgaa aacttatgaa
    23881 atgaaagacc ttttacatct tgcagaaatc ggtactggga ttaaactaag caataaaaac
    23941 taatgttccc ctcattcttt gaacttctac tatttgattt atttattctg ataaatgacg
    24001 atttcacctt caggatcttc aaatcccgta tagaatttat ttaagatctc ttcagtatca
    24061 gtaataggat aggcgatagg ccacataccc tttgatggtt agaataaact gctcaaaatc
    24121 tgtatcgtat gttggcttta gcatattaac aaaggctatc tccaaattat aaaccataag
    24181 cgttgaatcg ttcttaacaa tttcaattct tcctaaccca aagtcagaag atataattac
    24241 cggacttgcc ttacccttca tgtgggttgt tgagattcca tatttaaagg gaattgtcgg
    24301 tactatcggc ggtctgaaaa acatcccaaa aataaaaaaa tctttcccct gctagtctcc
    24361 taagtccctt tgtagcggat atggcaccat aatctcattt tatcgaagtg agtcatggtt
    24421 tatctgttgg ccaaccaaaa ttgatacaat ctaacgatgc accctctacg gcattctagg
    24481 ggagtgcact tgcattcacc caataaaaac cggcagttag caaagaactt atgtagtcgt
    24541 atgttcttac tgttaacata caagaggtct cctttccaca attgcttagc agtaaacggg
    24601 aatttacaaa gctaatctct tccattttct tatctttgtt ctaaaagtcc caaatggagc
    24661 aactgtattt acatgaataa atttatatac ttcccataca gccgttttgg ttgcctcgtc
    24721 tgcccatttt cttttgtgtg cagtgaatag atcttcatca ctcattgaat caatcatcat
    24781 gttaatatca tcaatattac gattgagaat ttcctctaat tctttcaatg gtagatgtgc
    24841 ataggtattt gtaaaccact tatataactc tcctaactga ttccatttaa attgatcaga
    24901 aggcgtttta acagataatc catttctttc atcattttcc cattttagaa ttaaattcgt
    24961 ccaaccaact tgatatgcta aattttcagc aggtgttcta tccacttcct caactctttt
    25021 atccttcaat tcttcaggaa tagtttcaaa ttctaaaata tatttagtat aagtcctatt
    25081 tatttctttt tttaattctt ctttattttt atatgttctc attatattac ctccataaat
    25141 tcctaattgt ctcctttaat tcatctataa tttcatccac gatattttaa tccgttctac
    25201 aatgcatatc tgatatgcat tgactgtcta actcatatgt gaatgattta taaaatatcc
    25261 caacgtctca tctaaagaaa tgttgatcgt cagcaaggag ctaaaaaata cgaatatatt
    25321 tcttttgtag taaaatcgga ctggtttgca tatcattttc ttcgtaaata tcaaaatcat
    25381 gttaactact atcaattact aataaaaact accactttaa gcgttaagaa ctgttaagtt
    25441 tagttaagtg aggatagttc acaaaatcca ttgttaaact tcacgaaagt ggtaaaactc
    25501 aaaatgcttt atataggatt acagcatcgc tctttcttcg attgcgcgtt gggtaaaaaa
    25561 tattatgaag taaaaattga cgatgacacg ttccttacgg ctcatcagat gaaacagcta
    25621 caaaaacgaa atgatcaact cgaagaaaat ttaattctaa aaaagcgatt gccatattca
    25681 cgcctcgctc gacgaactat taaaggtcaa gatttacctg aagatcatcc tttttaggaa
    25741 attgaagctt tttatgttgc acaatgcatt tataatacta caatgatgtt ttcaccagat
    25801 attcttctag ggtgcggtgt aatgaaacag atacacttgc aaaagaaaat tcaccgcgaa
    25861 tttactaaaa ttatgaataa ttatgttaat cttccgaaat ttttttccaa aattaaggga
    25921 tcaagctggt gttatagatt gtttaaggtt ggcaaaaaaa taacttcttt ttaaacaatt
    25981 atagaaaacc gatactctgt taagttggtt gacacggtgt cgatttttat gactttctta
    26041 ttctgaaact tcactaacgg acagtaccta aaaagtctat agtatggacg tttacgagta
    26101 ctattttatc aaaaatgctg ctcatttcaa cttatatgtt ttagtgaact acccccactt
    26161 a
//
LOCUS       DARBET010000031        25980 bp    DNA     linear   BCT 07-NOV-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4027
            SAMEA114569841-rid20112183.denovo.031, whole genome shotgun
            sequence.
ACCESSION   DARBET010000031 DARBET010000000
VERSION     DARBET010000031.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA114569841
            Sequence Read Archive: ERR12203666
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 25980)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 25980)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001972618.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001972618.1
            Genome Coverage       :: 132x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/07/2023 13:18:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,041
            CDSs (total)                      :: 2,975
            Genes (coding)                    :: 2,908
            CDSs (with protein)               :: 2,908
            Genes (RNA)                       :: 66
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 67
            CDSs (without protein)            :: 67
            Pseudo Genes (ambiguous residues) :: 0 of 67
            Pseudo Genes (frameshifted)       :: 26 of 67
            Pseudo Genes (incomplete)         :: 28 of 67
            Pseudo Genes (internal stop)      :: 20 of 67
            Pseudo Genes (multiple problems)  :: 6 of 67
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..25980
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA114569841-rid20112183.denovo.031"
                     /isolate="VRE4027"
                     /db_xref="taxon:1352"
                     /country="Denmark"
     gene            133..3660
                     /locus_tag="SCJ86_001487"
     CDS             133..3660
                     /locus_tag="SCJ86_001487"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345076.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PD-(D/E)XK nuclease family protein"
                     /protein_id="HEG1169030.1"
                     /translation="MSLQFILGNGKKDHRQVLIDEASAWLDQDASNQVFFLVPNYSKF
                     EQEQEILAEMRRRSGKKTFSTTNVQVFSFYRLAWYLLQQTTLLTGNELSESGSAMILR
                     QILEKNEEELTIFRGEINKRGFIRQLQELYSELRTGNIQPEDLMLLFGDSPNAEDQQL
                     KMKDLKLIFSAYDSELTQRALTNEDALSILANYMQTQDFNNVKFIVSGFSRINGQEYQ
                     LLQAMMEKGQLVVDLLLDRPYTADLPEVLTLFHETGKLYFRLFQSARERKVPILVDRF
                     APEQTRPTELQLLQTMWEETSSQKKAAVHKIGGQEHLHLWEAENPTEEVRQLAVEIRR
                     LVSEENYRYRDIQVLTKNMSLYGNILRPIFQEMAIPFYIDEEQMMENHPLIEWINSLF
                     ALDRYSYRLSDIMRFFKTELFLSTEAETELDEWKQERDEFRKKLDITENVALAYNYQG
                     TYWTREKDWQFVAYDFEAEQIEDVQYLEEQSNTIRRLFQKAVPVFLKKIKKSKTGQEA
                     ANVFYQFLIQSGVQQQLLFWRNQEVDRGNLEAARNHEQTWGALIDLLDEYVVIYGESA
                     FDWSLFQEIITSGLENLSYGKIPTAIDQVRINRLELVRPNQAAVTFALGLNDQVFPDR
                     KETKGLLSSEEREVLNQALPDGQFLFDPSKESISFEPFQAYLVFLSGTDRLYLSYAQS
                     YDTDGSLKMSPYLRRITEYLSVPVERKEHLTIESDPNCYVGTYRSGINTVNRIYRLAL
                     DEKKPVPKHWQKLKELVLNSSWRNFALQIFESQEHLNVPVSMPEKMAEELYGKEIYTS
                     ISRMENFYNCEYKYFMQFGLKLKERTVYGLTPAATGDFYHESLDRFFKLLFSNQLSLV
                     NMSDAQRRDFTEKVLQEVFGELRFEILDSSARMNYIRYQLGQTIQKVTWALQKQSKNT
                     GMHPLQTEILFGQIAGAKGITGLELPLSNGGKLHVRGKIDRIDVASEQEDTWLSVVDY
                     KSSGRSFDVTEAYYGMAMQLLTYLDVALMDAVRLTGKAAVKPAGSYYLHVHNPVLTET
                     DDVEKNTLKKFSYDGVFVDDPALLEVLDHSLEAKEQSLIYPVKKNAKDEYQKGSFSKD
                     KFFTEEELQLFMAHNRENMRLAGDAITSGEIKLNPAYKDKQRIACEFCPFRSICTFDV
                     MLKENNYHRLEKIDKEEALRRILKRSEEE"
     gene            3657..7379
                     /gene="addA"
                     /locus_tag="SCJ86_001488"
     CDS             3657..7379
                     /gene="addA"
                     /locus_tag="SCJ86_001488"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296885.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helicase-exonuclease AddAB subunit AddA"
                     /protein_id="HEG1169031.1"
                     /translation="MNQIPLKPQNERFTDDQWQAIFDQGDNLLVSASAGSGKTTVLVR
                     RVIEKLKMGFDIDELLIVTFTEAAAREMKERIQEALQESVNSESDPVRRQHFTKQLVL
                     LPTANISTLHAFCLTVIRRYYYLIDIDPVFRMLTDETETILMKEDVWDELREALYAEN
                     DERFFQLTMNFSNDRSDDGLTNLVFSLYEFARANPDPQKWLEQLSDNYRLPEGLAKSR
                     LYQKQIRPLVLADIYQCVQLYEQMTQLAQGEGLEKMHEQVAGEQQQIKNIYEAFSQDR
                     LEEAYAGLEQLTFSTFKSSRKAELKEISNEVKGMRDKAKKLIQQISKSYFPVSPSQME
                     ELTDKALPLVEEMTKVTQSFMDGFSMRKREKGVLDFNDLEHLALQILTEKTKDVWLPS
                     EASKHYRKKFKEVMVDEYQDVNQLQEAILYWLREPDDTKGNMFMVGDVKQSIYSFRLA
                     DPSLFIGKYENFSKKEGGRRIVLAENFRSRKEVLSFTNLIFEQLMDPAVGQINYDEAA
                     KLIQGFSDFPENEQFEPEIMIYEKEQEESEIEIPTDDILEDKTEGELFMTGLKIRQLI
                     DSSFMIYDKKSKKSRPIEYKDIVLLTPTKKNNLTILEIFKTLDIPLEMNDAQNYFQAT
                     EIRTMISLLQLIDNPYQDIPLAAVLRSPIVGLIEPELASIRLANRAHTYYDAVLAYQA
                     SNEDELAAKLEHFGKQLEHWRELARRSSITDLLWDIYYETGYLEYVVGLPAGAQRQAN
                     LYALVDRAKAYEQSSFRGLYQFVRFIEKMQEKDKDLAEPVISIEDNAVRVMTIHASKG
                     LEFPVVFLLDMTKEFNLQDLRNRYAFEEKLGAGIRYMDPETRVLYDTLPFQAIKLAKQ
                     NKLLSEEMRKLYVGLTRAEQKLFIVGSYKNKEQMIQTWSEAADHEELVFDPALRLKGR
                     SSLMNWIGYGLIRHPEMQKYLEEEISTSLLQHSNAQFSISWMNQQSIIEQRQLLAEKE
                     LVNLDQQMKEDETLLADSLQKRLAYEYPYQASSQTTSYQSVSEIKRLFEDPDDTQESR
                     LTLESSQNKAASRQFRYTQEQLAEPKFLQKDRQVSAAAVGTATHALLQLLPLEMPTTE
                     SIHQKLQELVRKRLVDEKVAKKVDVSSIIWFFQTELGQQLIANKENVKREQPFSMLLP
                     ADEVFQDYPNQEDELLIHGIVDGYLEEKDHLNIYDFKTDFILHPDDPAEIDAIVQKYQ
                     GQLRLYQQAMSEALNKPVENVFLILLRVKQIININK"
     gene            7448..7789
                     /locus_tag="SCJ86_001489"
     CDS             7448..7789
                     /locus_tag="SCJ86_001489"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HEG1169032.1"
                     /translation="MELTQTEFVLCPKFEKTFSILGKKWNGLIIDVLLENGPQRFVEL
                     AAKIPMVSDRVLVERLKELEKEGIVTRNFACSEKSRSEYLLTEKGADLQKAMRQIQVW
                     SEKWITEEECG"
     gene            8098..9141
                     /gene="pheS"
                     /locus_tag="SCJ86_001490"
     CDS             8098..9141
                     /gene="pheS"
                     /locus_tag="SCJ86_001490"
                     /EC_number="6.1.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016171868.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phenylalanine--tRNA ligase subunit alpha"
                     /protein_id="HEG1169033.1"
                     /translation="MSLQAQLESLKEATLVKITAAQDLNELNQIRVETLGKKGPITEV
                     LRGMKDLSPEERPKVGSFANEIRDLLTEEIERKKQVLEEEALNAALAKETIDVTLPGR
                     KVKHGTRHIVTQIMEEIEDIFVGMGYQIVEGYEVESDHYNFERMNLPKDHPARDMQDT
                     FYISDEILIRTHTSPVQARTMEKHDFSKGALRMISPGKVFRRDTDDATHSHQFHQIEG
                     LVVDKNITMGDLKGTLEVVMKKMFGEDREIRLRPSYFPFTEPSVEVDVSCFKCGGAGC
                     NVCKYTGWIEILGAGMVHPNVLKMSGIDPEEYSGFAFGLGPDRVAMLRYGVNDIRNFY
                     QNDLRFLSQFKGE"
     gene            9146..11566
                     /locus_tag="SCJ86_001491"
     CDS             9146..11566
                     /locus_tag="SCJ86_001491"
                     /EC_number="6.1.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phenylalanine--tRNA ligase subunit beta"
                     /protein_id="HEG1169034.1"
                     /translation="MLVSYKWLNRYVDLSNVTPKELADKMSVTGIEVEGVTVPEEGLK
                     KIVVGEVKECVPHPDSDHLSICQVDIGEDELSQIVCGAPNVKVGIKVIVALPNSRIAG
                     NVKIKKGKMRGQVSNGMICSLQEIGYSDSVIPKEYAEGIYYMPQEAVNGDPVFPYLDM
                     DDSIIELSITPNRADALSMRGVAYEVGAIYRQTPTFDDEKLVESSRPASDKIQVEVED
                     KEAVPAYQIRIIEGVRIQPSPQWLQNLLMNEGIRPINNVVDVTNYILLLFGQPLHAFD
                     YDKLKSKEIFVRRAKENETLVTLDGESRELTTDNLVITNGKEPVALAGVMGGLDSEIT
                     NETTTVALESALFDPISIRRTAKQFNLRSESSARFEKGINHATVGEASEVAAAMIVSL
                     AGGEVLQGAVKGSEIKAEDVSVKISLERINQYLGTSLVVKEVNEIFEALGFSYEEKEY
                     VYSVVVPPRRWDIQIEADIIEEVARIYGYDQLPSTLPSGETVAGSLSKEQETTRKIRT
                     IFEGSGLSEAISYALTTEEKSRQFVINESNITRLDWPMSEERSVLRMNLISGLLDDIS
                     YNTARKNTEIALYEIGRVFFQENDPRTHLPQEVKHAAIAISGIWEEKDWQTKAQNVDF
                     FTIKGLLENLFEQLGITEDVHYQTVTEMKEMHPGRTAAIYLGEKFIGFVGQVHPTITK
                     SYDIPETYVAEFDLSAIVEAAQKGIVFEAVTKFPAVSRDIALLVKETVTNQELTEVIR
                     SAAGRFLTDIQLFDVYQGENIEKGHKSMAYSLTFVNPEATLTDEEINKAMEKVEKALV
                     ENLEASIR"
     gene            complement(11736..12746)
                     /gene="trpS"
                     /locus_tag="SCJ86_001492"
     CDS             complement(11736..12746)
                     /gene="trpS"
                     /locus_tag="SCJ86_001492"
                     /EC_number="6.1.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288045.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan--tRNA ligase"
                     /protein_id="HEG1169035.1"
                     /translation="MKNIILTGDRPTGKLHLGHYVGSLKTRVAMQADEDNQLFVMIAD
                     MQALTDNAKNPEKVSSNVLQVALDYLAVGLDPAKSTLFIQSQIPELAELTMYYLNLVS
                     VGRVRRNPTVKTEIEQKKFGESVPTGFFIYPVSQAADITAFKANLVPVGEDQKPMLEQ
                     TQEIVQSFNHTYGEVLVEPKGVFPPKGMGRLPGIDGNGKMSKSLGNGIYISDPADVLQ
                     KKVMSMYTDPNHIHVQDPGQVEGNMVFTYLDVFGTDKEAIEEMKAHYRRGGLGDVKIK
                     RYLIDVLEAEFAPIRARREELEKDPSAVMEILRKGSEEAAKVAAQTLSEVKSAMGINY
                     FH"
     gene            13000..13737
                     /locus_tag="SCJ86_001493"
     CDS             13000..13737
                     /locus_tag="SCJ86_001493"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002364205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter ATP-binding protein"
                     /protein_id="HEG1169036.1"
                     /translation="MAMIKFDHVDKYYGKFHALKNINLEFEKGEVVVVIGPSGSGKST
                     MLRCINGLETISSGKLLINDTDLHDKKTKLTEVRKNIGMVFQHFNLYPNKTVLENIKL
                     APIKVLKQDQATAVKNAEKFLKTVNMLDKKDSYPSMLSGGQQQRVAIARGLAMNPEML
                     LFDEPTSALDPEMIGDVLDVMKKLAHDGMSMIVVTHEMGFAKEVADRVIFMADGEVLE
                     DSRNVRDFFENPNEVRAQQFISKVINH"
     gene            13751..14566
                     /locus_tag="SCJ86_001494"
     CDS             13751..14566
                     /locus_tag="SCJ86_001494"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292956.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transporter substrate-binding domain-containing
                     protein"
                     /protein_id="HEG1169037.1"
                     /translation="MRRLKKIRLIFLLGIAFLALAGCKGNGLANEDILERSKSTNEII
                     WGVKYDTRLFGMMDIESRTVQGFDVDIAKAITKKILGDNGKTEFVEVTSKTRIPLLKN
                     GNIDAIIATMTITDERKKQVDFSDVYFDAGQALLVKKGSQIKSVDDLNASTTVLAVKG
                     STSAANIRQHAPDAKILELENYAEAFTALQSGQGDAMTTDNAILLGIADENPEYELVG
                     GTFTNEPYGIAINKGQENFLKAVNQALEEMHADGTYDKIYQKWFPNETEGKVE"
     gene            14579..15226
                     /locus_tag="SCJ86_001495"
     CDS             14579..15226
                     /locus_tag="SCJ86_001495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter permease"
                     /protein_id="HEG1169038.1"
                     /translation="MSELIQTYGPIFVDGFKITLFSSVLALVFSLIIGTLMAIFQLSK
                     NKVVSALAKAYVEFFRNIPLLIIVMFFYVVVPIYWFNLDGFQAGTIGLTIYTSAFIAE
                     TVRSGIQTVPKGQTEAGLSSGFTYSEIMRYIVLPQAFKIVIPPLGNQFINLVKNSSIL
                     AIVAGLDLMYQGDLIASETFNTFDTYIIVGLFYLVITLPLSYLMVYLEKKWAVRS"
     gene            15242..15898
                     /locus_tag="SCJ86_001496"
     CDS             15242..15898
                     /locus_tag="SCJ86_001496"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631187.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter permease"
                     /protein_id="HEG1169039.1"
                     /translation="MDFSGAFSWMNIRFLLEGLKVTVEVSLFSIIFSFLIGGLTGVIR
                     FANIPFFSKILGVVIDIIRNLPLLLIIFFTYFALPQIGIQMNIFWSAVAALTIFESAM
                     LSEIFRAGLNAVPKGQMEAGLSTGLTYVESMRTIVLPQAFKSMIPAIVSQLISLIKDT
                     SLAVIISLPELSHQARIIYGQNTNYVLPMFIIMTLMYFVVCYALSLLSGYLEKRRYSY
                     "
     gene            16060..16881
                     /gene="racE"
                     /locus_tag="SCJ86_001497"
     CDS             16060..16881
                     /gene="racE"
                     /locus_tag="SCJ86_001497"
                     /EC_number="5.1.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376926.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate racemase"
                     /protein_id="HEG1169040.1"
                     /translation="MIRLTDNRPIGFIDSGVGGLTVVKEALKQLPNENILFVGDTARC
                     PYGPRPAEQVIQYTWEMTDYLVEQGIKMLVIACNTATAVALEEIKAALSIPVIGVILP
                     GTRAAVKKTQNKQVGIIGTIGTVKSQAYEKALKEKVPELTVTSLACPKFVSVVESNEY
                     HSSVAKKIVAETLAPLTTKKIDTLILGCTHYPLLRPIIQNVMGENVQLIDSGAETVGE
                     VSMLLDYFNLSNSPQNGRTLCQFYTTGSAKLFEEIAEDWLGIGHLNVEHIELGGK"
     gene            16884..18239
                     /gene="rph"
                     /locus_tag="SCJ86_001498"
     CDS             16884..18239
                     /gene="rph"
                     /locus_tag="SCJ86_001498"
                     /EC_number="2.7.7.56"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629501.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease PH"
                     /protein_id="HEG1169041.1"
                     /translation="MRHDGRKAQELRKLELQTNVFKYPEGSVVIRFGDTTVICSATIE
                     NSVPPFLRETGTGWVTAEYSMLPRATSTRNRRESSKGKLSGRTMEIQRLIGRSLRAVV
                     DLEKLGERSIIVDCDVIQADGGTRTASITGAFVALKLAIEKLLREKELSEDPIKEHLA
                     AVSVGILPDGTCVTDLDYQEDSAALVDMNLVMTESGKFVEIQGTGEEATFDGEQLNEM
                     LFFGKNAIEDLIKEQKHALLTEFAQNDERIEETKTIIIATRNPGKAEEFRNMFKEAGY
                     HVKTLLDYPELPDVEETGSTFEENARLKAETIAQLLDQPVLADDSGLKVDALGGMPGI
                     YSARFAGEQKSDAGNNAKLLYELTDVPDEKRTAQFHCTLVFAAPKKDSLVVEAEWPGR
                     VARIPSGENGFGYDPLFIPEGKKQTAAELSSEEKNKISHRAQAMKKLSAEWKQWLEGE
                     R"
     gene            18240..18758
                     /locus_tag="SCJ86_001499"
     CDS             18240..18758
                     /locus_tag="SCJ86_001499"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345913.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase"
                     /protein_id="HEG1169042.1"
                     /translation="MRYLVVSDNHGDRDILVDLVDRYRSEVDIFFHCGDSELEASDPL
                     WDTFQVVQGNCDYGPGFEQKKVIQTGQDTVFMTHGHLSNVRFGLTQLAIEARAATADM
                     VFFGHTHQIGCEMDQKILFLNPGSISQPRGPIQIPSYALIESLDDQISVQYYNRAHQA
                     VEELAFSFSRER"
     gene            18865..19365
                     /locus_tag="SCJ86_001500"
     CDS             18865..19365
                     /locus_tag="SCJ86_001500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328417.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CBS domain-containing protein"
                     /protein_id="HEG1169043.1"
                     /translation="MIGPIVREMLLENQETFLIPAENVANVMYHHPLSHGLLVLSKVG
                     YSKIPVLGKDDHFVGLVSLSDVVNKMIDLQTISMDPLEGLTVADVTETDVPTIDENWE
                     LEEVLHLLVDSPFLPVVTEDRIFKGIITRKELLKAVNFMVHELERRNILSPKIEELKE
                     QIDIVS"
     gene            complement(19645..20565)
                     /locus_tag="SCJ86_001501"
     CDS             complement(19645..20565)
                     /locus_tag="SCJ86_001501"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339341.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mechanosensitive ion channel family protein"
                     /protein_id="HEG1169044.1"
                     /translation="MFSLLATAGTTDSSGETLTDQAVSKVNAFQRFWDGIDWDAILAA
                     FIQKSLYLIFLIVLFWILHRVGKYLIDKSFNQYSKKAILNESRLKTLHSLLNTIFHYT
                     LGFFFIYAILSAIGVPIGSLLAGAGIAGVAIGLGAQGFMSDVITGFFIIMEQQMDVGD
                     YIKLANLTIEGTVVSVGIRTLQLKATDGTVHFIPNRNITTISNLSRANMQVLLDIRIV
                     PEEGYDKIYEIIDRVNRTLAEKYQEELQTEPSIFGLVDLGNSNFAIRTVCYALNGKQY
                     ALKEEFLSSYVKELTASGFTIPNSPIAVGK"
     gene            20730..21743
                     /locus_tag="SCJ86_001502"
     CDS             20730..21743
                     /locus_tag="SCJ86_001502"
                     /EC_number="2.6.1.42"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735585.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid aminotransferase"
                     /protein_id="HEG1169045.1"
                     /translation="MQIDWNNLGFSYIKTPWRFIAKWKDGQWEEGELTEDNYLTIHEG
                     SPALHYGQQCFEGLKAYRRKDGKVNLFRPEQNSRRMNQSARRLLMPMVPEKMFVDAVK
                     QVVKANEDFVPPYGTGATLYLRPLLIGVGENIGVHPAPEYLFVVFCTPVGAYFKGGLK
                     PTNFIVSDYDRAAPKGTGAAKVGGNYAASLLPGTEAKELDYSDCVYLDPATHTKIEEV
                     GAANFFGITKDGKFITPQSPSILPSITKYSLLQLAEERLGLQAIEGDIFIDDLDQFAE
                     AGACGTAAVISPIGGIYHNNNLHVFYSETEVGPVTKRLYDELTGIQFGDIEAPEGWIE
                     VVE"
     gene            complement(21853..22608)
                     /gene="fabI"
                     /locus_tag="SCJ86_001503"
     CDS             complement(21853..22608)
                     /gene="fabI"
                     /locus_tag="SCJ86_001503"
                     /EC_number="1.3.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005877106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-ACP reductase FabI"
                     /protein_id="HEG1169046.1"
                     /translation="MSFLEGKKIVIMGVANKKSIAWGCAEAMLKQGAEIIYTYQNERM
                     KKSLVKLVGEEAFTVECDVSSDESIEKAMNAIGEYAGEIHGLVHAVAYANKEELSGNV
                     SDISRDGYQLAQDISSYSLIAVSKYAQPFLAPNSGIVSMTYLGSERAIPNYNMMGIAK
                     ASLEAAIRYLAAEFSPKGIRVNGISAGAIKTLAVTGVKDYQKLIQLSESRTPDQQGVT
                     IEEVGNACAFLISPLSSGIIGDIIYVDKGVHLS"
     gene            22828..23268
                     /gene="fabZ"
                     /locus_tag="SCJ86_001504"
     CDS             22828..23268
                     /gene="fabZ"
                     /locus_tag="SCJ86_001504"
                     /EC_number="4.2.1.59"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-hydroxyacyl-ACP dehydratase FabZ"
                     /protein_id="HEG1169047.1"
                     /translation="MSYVLSATEVMELIPNRYPICYIDYVDTLEPGKKIVATKNVTIN
                     EDFFQGHFPDNPVMPGALILETLAQAGSILILKSEEFLGKRAYIGGINKAKFRQKVVP
                     GDVLKCHFEITKMKGSVGTALSAAYVDDKKVCECEFTFIVNQNA"
     gene            complement(23383..24144)
                     /locus_tag="SCJ86_001505"
     CDS             complement(23383..24144)
                     /locus_tag="SCJ86_001505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376918.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HEG1169048.1"
                     /translation="MKKNKVILSISLAAALLFAAGCSANNTTSSSSSTESSKSTSTAE
                     STTASSVDLNALELPQLTKDVAENEDLVEMVTNKGTIKIKLFPEHAPKAVENFMTHAK
                     DGYYNGLTFHRVIKDFMIQGGDPKGDGTGGESIWGEGFETEINNHLYNIRGALSMARS
                     QDPNSNGSQFFIVQNTDDMHDGLLKDDYPQAIIDAYKNGGYPSLDGHYTVFGQVIEGM
                     DVVDAIANIETDSSDKPKEEVKIDKINILQEAKQK"
     gene            24333..25427
                     /locus_tag="SCJ86_001506"
     CDS             24333..25427
                     /locus_tag="SCJ86_001506"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318729.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF871 domain-containing protein"
                     /protein_id="HEG1169049.1"
                     /translation="MGKLGISIYPERSTFEKDKAYLDLAHKYGYKRVFTSLLQINDDK
                     EKVLSEFKEVVDYANSLGMEVMVDINPALFEQLEISYDDLSFFHKMGAYGVRLDIGFT
                     GAEEAKMTRNPFGIKIEINMSSGTNYVDNIMSYSPNTDNLLGSHNFYPHRYSGLGYEH
                     FVFCSEKFRKYNLNTMAFVNSQSAEFGPWPTQDGLCTLEDHRDLEIATQVKHLILTGL
                     IDDISIGNAYASEEELKEMAEAFNADYPTLKVDTEEGITENERICLFDNLHSYRGDRS
                     EYILRSTMTRVYYKDKDFPPHNTRDMHHGDVLIDNEGYGQYKGETQIALKDMKNDGRV
                     NVVGRISDDELFLLDFLKPWSSFKLIENKK"
     gene            complement(25513..>25980)
                     /locus_tag="SCJ86_001507"
     CDS             complement(25513..>25980)
                     /locus_tag="SCJ86_001507"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000221326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS30 family transposase"
                     /protein_id="HEG1169050.1"
                     /translation="GDTIVGIHHKSAVITLVERLSKVIITIKPNGRKALDIETALNQW
                     FSRFPKNFFKSITFDCGKEFSNWKAISNQHDIDIYFADPGTPSQRPLNENSNGILRRN
                     GLPKSMDFRKVNQTFISSVSNQRNHIPRKSLNYRTPIEIFLSYVQEAFYSSLI"
BASE COUNT         8596 a         4501 c         5474 g         7409 t
ORIGIN      
        1 tttttgcgta tacttaaaaa agtagaaata tcagaacctt aaaaggatat aatttgaaga
       61 atatagaaca aatgttcagt attttttgat ataataaagg agcaccgtat catgtgttga
      121 aggaggaaaa gcatgtcttt acaatttatt cttggaaacg ggaaaaaaga tcaccgtcaa
      181 gtcttgatcg acgaagcatc cgcttggctc gaccaggatg caagtaacca agtatttttc
      241 cttgttccca actacagcaa attcgaacag gaacaagaaa ttttagcgga gatgcgaaga
      301 agaagcggaa aaaaaacttt cagcacaaca aacgttcaag tatttagttt ttatcgtctg
      361 gcttggtatt tgctgcaaca gacaactctt cttacaggaa atgaattatc agaatcagga
      421 tctgcaatga tcttgcgaca gattttagaa aaaaatgagg aagaattaac aatctttcgc
      481 ggagaaatca ataaaagagg gtttattcgt caattacaag aactctacag tgaattgcgt
      541 acaggaaata ttcaaccaga agacctgatg cttttatttg gtgattcacc taatgcagaa
      601 gaccagcaac ttaaaatgaa agacttgaag ctgatttttt cagcttatga cagcgaattg
      661 actcaacgtg cactaacaaa tgaagatgca ctatctatac tagcaaatta tatgcagaca
      721 caagatttca acaacgtcaa atttattgtc agtggttttt ctcgaatcaa tggtcaagag
      781 tatcaattac tgcaggcaat gatggaaaag gggcaattgg ttgtcgattt attgctggat
      841 cgtccttata cagcggatct tccagaagta cttacgctct ttcatgagac tggaaagctt
      901 tatttccgtc tttttcaaag tgcaagagaa cggaaagtcc ctatattagt ggaccgattt
      961 gcaccagagc agactcgtcc gacagagctt caactgctgc aaacaatgtg ggaagagaca
     1021 tctagccaaa agaaagcggc agtacacaaa atagggggac aagagcatct ccatttgtgg
     1081 gaagctgaaa atccaactga agaagtccga caattagctg ttgaaatcag acgactagtc
     1141 agtgaagaaa actaccgtta tcgtgatatc caagtgctga caaaaaatat gtctttatat
     1201 ggaaatatcc ttcggccgat ctttcaagaa atggcaattc ctttttatat cgatgaagaa
     1261 caaatgatgg aaaatcatcc gctgatcgaa tggatcaaca gtctgttcgc tcttgaccgc
     1321 tattcttatc ggctaagtga tattatgcgt tttttcaaaa ctgaattatt tctgtcgacg
     1381 gaagcagaaa ccgaacttga tgaatggaaa caagagcgag atgaattcag gaaaaaactg
     1441 gacatcactg aaaatgtagc acttgcttac aattatcaag gaacttattg gactcgtgaa
     1501 aaagactggc agtttgtggc gtatgatttt gaagcagaac agattgaaga cgtacagtat
     1561 ttggaagaac agtcaaatac gattagacga ttatttcaga aagctgtacc agtatttttg
     1621 aaaaaaataa agaaaagcaa aacaggacaa gaggccgcaa atgtttttta tcaatttttg
     1681 atccaaagtg gcgtacagca acaattattg ttttggcgta atcaggaagt agacagagga
     1741 aacttagaag cggcaagaaa ccatgagcag acatggggag cgctgattga tctgttggat
     1801 gaatatgtag taatttatgg tgaatcggct tttgactggt cactgttcca agaaatcatt
     1861 acaagtggtc tagaaaatct aagttatggt aaaatcccta ccgcaatcga ccaagtgcga
     1921 atcaatcgtt tggaattagt tcgaccaaat caagctgccg tcacttttgc attagggttg
     1981 aatgatcaag tgtttccaga ccgaaaagag acaaaaggat tgttgtccag tgaagaacga
     2041 gaagtattaa atcaggcctt gccagatgga cagtttttat tcgacccatc aaaggaaagt
     2101 atctcttttg agcctttcca agcctatctt gtttttcttt ccggaacaga tcgattatat
     2161 cttagttatg cacaaagcta tgacacagat ggttcgctga agatgtctcc ttatttgcgt
     2221 agaatcacag aatatttatc tgttcccgtc gagagaaaag agcatttgac gatcgaaagc
     2281 gaccctaatt gttatgtagg gacttacaga agcggaatca atacagtcaa taggatttat
     2341 cgattagctt tagatgaaaa aaaacctgta ccaaagcact ggcaaaagct caaagaatta
     2401 gtactgaatt cgtcatggcg aaattttgct ttacagattt ttgaaagtca ggaacattta
     2461 aatgttcctg tttcgatgcc agaaaaaatg gcggaagagc tttatggaaa agagatttat
     2521 acttcaattt cacgtatgga gaatttttat aattgtgaat acaaatactt catgcaattt
     2581 ggtttgaaat tgaaagaacg aactgtttat ggtctaactc cagcagcaac tggtgatttt
     2641 tatcatgaat cattggatcg tttcttcaag ctactgtttt caaaccaatt atccttagtg
     2701 aatatgtccg atgcgcagcg tagagacttt actgaaaaag tgctgcagga agtattcgga
     2761 gaacttcggt ttgagatatt ggatagttct gcaagaatga actatatccg ctaccagtta
     2821 ggacagacga ttcaaaaagt gacatgggct ttgcaaaaac agagcaaaaa tacaggcatg
     2881 catcctcttc agacagagat tctatttggt cagattgctg gagcaaaagg tatcacaggt
     2941 ctggaattac cgttgagtaa tggtggaaaa ctccatgttc gagggaaaat cgatcgaatc
     3001 gatgtagctt ctgaacaaga agatacttgg ctaagcgtcg ttgattacaa atctagcgga
     3061 cgttcattcg atgtcacaga agcttattat ggcatggcta tgcaattatt gacgtatctg
     3121 gacgtagcat tgatggatgc ggtacgtttg actggaaagg cggctgttaa accagcagga
     3181 tcatattatt tacatgttca taatcctgta ttgacggaga cagatgatgt agagaagaat
     3241 acattgaaaa aatttagtta tgatggcgtt tttgtcgatg atcccgctct tttagaagta
     3301 ctagatcatt cactggaagc aaaagaacaa tcactgatct atccagtgaa aaaaaatgcg
     3361 aaagatgaat accaaaaagg tagtttcagt aaagacaaat ttttcacgga agaagaactg
     3421 caactattca tggcgcataa tcgggaaaat atgcggttag ctggagatgc gattacttcc
     3481 ggagaaatca aactaaatcc agcttataaa gataaacagc ggattgcctg cgaattttgt
     3541 ccattcagaa gcatctgtac gtttgatgtg atgctgaaag aaaataatta ccaccgtctg
     3601 gagaaaatag ataaggaaga agcactgcgt agaatattga aaaggagtga agaagagtga
     3661 atcaaattcc tttaaaaccg caaaatgaac gattcacaga tgatcagtgg caagcgattt
     3721 ttgatcaagg agataatcta ttagtctctg cgtctgccgg atcaggtaaa actacagtgc
     3781 ttgttcgaag agtgattgaa aaactaaaaa tgggttttga tatcgatgaa ttattgattg
     3841 tgacatttac tgaagctgcc gcaagagaaa tgaaagaaag aattcaagag gcattacaag
     3901 aatccgtcaa tagcgaaagt gaccctgtac gtagacagca ttttactaag cagttagttt
     3961 tattgcccac agcgaatatc tcaaccttac atgctttttg cctgacagtt attcgacgtt
     4021 attattactt aattgatatt gatccagtct ttcgcatgtt aacagatgaa acagaaacga
     4081 tcttgatgaa ggaggatgtg tgggacgagc tgagagaagc actttatgca gaaaatgatg
     4141 aacgattttt ccaattaaca atgaattttt ctaatgatcg ttcagatgac ggcttgacga
     4201 atctggtgtt ttctctttat gagtttgccc gtgccaaccc tgacccccaa aaatggctag
     4261 aacagttaag cgataattat cgtttgcctg aggggctagc gaaatctcga ttatatcaaa
     4321 aacaaatccg tcctttagtt ttggcagata tataccaatg tgtacagtta tatgaacaga
     4381 tgactcagtt agcacaaggt gaaggattag aaaaaatgca tgagcaagta gctggagaac
     4441 aacagcagat caagaatatc tatgaagcgt tctcgcaaga tcggttagaa gaagcttatg
     4501 ctggtttgga acaactaact ttttcaactt ttaagagcag tcggaaagca gaattgaaag
     4561 aaatttccaa tgaagtcaag ggcatgaggg ataaagcaaa aaaactgatt cagcagataa
     4621 gtaaaagtta ttttcctgtt tccccaagcc aaatggaaga attaacggac aaagcactac
     4681 cgttagtcga agaaatgaca aaagtcacgc aaagcttcat ggacggtttc agtatgcgta
     4741 aacgggaaaa aggtgtgctg gatttcaatg atctggaaca tttagcgttg cagattctca
     4801 cagaaaaaac gaaagatgta tggctgccaa gtgaagcttc aaaacattat cgaaaaaaat
     4861 tcaaagaagt aatggtagat gaatatcaag acgtgaacca attacaagaa gccattttat
     4921 actggctaag agaacccgat gatacaaaag gaaatatgtt catggttggg gatgtcaaac
     4981 aatccatcta ttctttccgt ttagcagatc ctagtttatt tatcgggaaa tacgagaatt
     5041 tctcaaagaa agaaggcgga cgtcgaatcg ttttggctga gaacttccgc tctagaaaag
     5101 aagtcctttc ttttacgaat cttatttttg aacaactaat ggatccagca gtgggtcaaa
     5161 tcaattatga cgaagcagcc aaactgatcc aaggattttc agactttcca gagaatgaac
     5221 agtttgagcc ggaaatcatg atctatgaaa aagaacagga agaatcagag attgagattc
     5281 cgacggatga tatactggaa gacaaaacag aaggcgaact ttttatgaca ggactaaaaa
     5341 ttcgtcaatt aattgattcc tcctttatga tttatgataa aaaatcaaaa aaaagtcgtc
     5401 cgatagagta caaagacatt gttctcttga cacctacaaa gaagaacaac ttaacgattt
     5461 tagagatttt caaaacattg gatattccat tagagatgaa tgatgctcaa aactattttc
     5521 aagcgacaga aattcgcacg atgatctcgc tgctacaatt aatcgataat ccctatcaag
     5581 atattccgct tgcggctgta ttgcgctcac cgatcgtggg tctgattgaa ccagaattag
     5641 ccagcattcg attagctaac cgtgcacata cttactatga tgctgtgctt gcttatcaag
     5701 catcaaacga agatgaatta gcagctaagc tggaacattt tggcaagcaa ctagagcatt
     5761 ggcgagaact tgctcgccgt tcttcgatca ctgacttgtt atgggatatc tattatgaga
     5821 cgggatatct agagtatgtg gtcggtctgc cagcaggggc acagagacaa gccaatcttt
     5881 atgcattggt tgatcgtgcc aaagcttacg agcaaagttc tttcagagga ttataccagt
     5941 tcgtgcgttt cattgaaaaa atgcaagaaa aagataaaga cttagcagaa ccagtcattt
     6001 caatagaaga taatgctgtt cgcgttatga cgatccatgc aagtaaaggc ttggaattcc
     6061 cagttgtatt tttgttagat atgacaaagg agttcaactt acaagacttg cgcaatcggt
     6121 atgcttttga agaaaaactt ggagcaggaa ttcgctatat ggatcctgaa acacgagtgt
     6181 tatatgatac gttaccgttt caagccatca aattggcaaa gcaaaacaaa ttgttgtcgg
     6241 aagaaatgcg taaattgtat gtaggattga ctcgggcaga acaaaagctt ttcattgtcg
     6301 gttcctataa aaataaagag caaatgatcc aaacgtggtc tgaagcagct gatcatgagg
     6361 agttagtttt tgatcctgcg ctacgtttga agggcagaag cagcttgatg aattggattg
     6421 gttacggatt gatccgtcat cctgaaatgc agaaatactt agaggaagaa atttcgactt
     6481 ctcttcttca acattcaaat gcacagtttt ctatttcatg gatgaatcag caatcgatca
     6541 ttgaacaaag acagttgctt gctgaaaaag aactggttaa cttagatcag cagatgaaag
     6601 aagatgaaac tttattagca gattcgttac aaaaacgtct ggcatatgaa tatccttatc
     6661 aagcttcttc acaaaccaca agttatcaat ctgtatctga aatcaaacga ttattcgaag
     6721 atccagatga tacacaagaa tcacgtttga ctctggaaag cagtcagaac aaagcagcat
     6781 cccgtcaatt tcgttatacg caagaacaac tagcagaacc aaaatttttg caaaaagatc
     6841 ggcaagtgtc agcagctgct gttggaactg ctacgcatgc tttgttacaa ttactgccat
     6901 tagagatgcc aactacagag agtatccatc aaaaattaca ggaattggta aggaaacggt
     6961 tagtagacga aaaagtcgcg aaaaaagtag atgtttcttc tattatatgg ttctttcaaa
     7021 cagaacttgg tcaacagctg attgccaaca aagaaaacgt aaaacgagaa caaccttttt
     7081 ccatgctgct tcctgctgac gaagtatttc aagactatcc aaatcaagaa gatgaactat
     7141 taatccacgg aatcgtcgat ggatacctag aagaaaaaga tcatctcaat atctatgatt
     7201 ttaaaacaga ttttatttta catccggatg atccagcaga aatagatgca atcgtccaaa
     7261 aatatcaagg acagctaaga ctttaccaac aagcaatgtc agaagctttg aataagccag
     7321 tagaaaatgt tttcctaatt ttgcttcgag tgaaacaaat tataaacatt aacaaataat
     7381 tataattgag caaacttgac ttatgttttg taaggattat gctatactct gggaagaggt
     7441 gagtatgatg gagctaacac agacagaatt cgttttatgc cctaaatttg aaaaaacatt
     7501 ctctatttta ggaaaaaaat ggaacggatt gattatcgat gtactactcg aaaacggtcc
     7561 gcaacgcttt gttgagcttg cggcaaaaat cccgatggtc agtgatcgag tattagtcga
     7621 acgcttgaag gaattagaaa aagaaggaat agtcactcga aactttgcct gtagcgaaaa
     7681 gagccgttca gaatatctct tgactgaaaa aggagcagac ttgcaaaaag cgatgcgtca
     7741 aatacaagtt tggagcgaaa aatggatcac agaggaagaa tgcggttgat tttgtaaaaa
     7801 aaatcttgta aactatagtc catacgaata agtaaagaac gatgacgaaa aagagtagtt
     7861 tggctaaacc agttcaggga gatctgtcgc agactgaaag cagatcctgt gtttgaaaaa
     7921 tgaagttcac tttccgagtt cgtctgataa aatcagaccg gcaattgtcg ttaacgaagt
     7981 aagagtgcgg tgtaagtcat ttatccgcaa attaggatgg taacgcgaag acctcgttcc
     8041 ttaacaggaa tgaggtcttt ttgttttgta tttggaattt aaaggaggaa caagcacatg
     8101 tctctacaag cacaattaga gagcttgaaa gaagcaacgc tcgtcaaaat cacagcggca
     8161 caagatttaa atgaattaaa tcaaatacgt gttgaaacgt taggaaaaaa aggaccgatc
     8221 acagaagtat tacgtgggat gaaagatcta agtccagaag agcgaccaaa agtcggaagt
     8281 ttcgcaaatg agatccgaga tcttttgaca gaagagatcg agcgaaaaaa acaagtatta
     8341 gaagaagaag cgttaaatgc agctttggca aaagaaacaa tagacgtgac actacctggt
     8401 agaaaagtaa aacatggtac acgtcatatc gtgacacaaa tcatggaaga aattgaagat
     8461 atttttgtgg gtatgggcta tcagatcgta gaaggatatg aagtagaatc tgatcattac
     8521 aatttcgaac ggatgaatct gccaaaagat catccagcaa gggacatgca agatactttc
     8581 tatatttcag acgagatatt gattcggaca catacttcac cagtccaagc acggacaatg
     8641 gaaaaacatg atttctctaa gggtgctcta cgaatgatct cgccagggaa agtcttccgc
     8701 agagatacag atgatgcgac ccacagtcat cagttccatc aaattgaagg gctagttgtt
     8761 gataaaaaca tcacaatggg cgaccttaaa ggaacattag aagtcgtaat gaaaaaaatg
     8821 tttggggaag atcgtgaaat tcgtttgcgt ccaagttatt tcccatttac ggaaccatca
     8881 gtagaagtag atgtcagctg tttcaaatgt ggtggtgccg gttgtaacgt atgtaaatac
     8941 actggatgga tcgaaatttt aggagctggc atggtacacc cgaatgtgtt gaagatgtca
     9001 ggaatcgatc cagaagaata ttcaggcttt gctttcggcc ttggaccaga ccgcgtagct
     9061 atgttgcggt atggagtgaa tgatatccgt aatttctatc aaaatgactt acgtttctta
     9121 agtcagttca agggggagta agaaaatgtt agtttcatat aaatggctca atcgttatgt
     9181 agatttatcg aatgtaacac caaaagaatt agcggataag atgtctgtga caggaatcga
     9241 agtagaggga gtaacggttc cagaagaagg cttgaaaaaa atcgttgtcg gtgaagtcaa
     9301 ggaatgcgta ccacatccag attctgatca cctttctatt tgtcaagtcg atattggcga
     9361 agacgaactg tctcaaatcg tttgtggagc gcctaatgtg aaggttggaa tcaaagtgat
     9421 tgttgcactt cctaactcac gtatcgctgg taatgtaaaa atcaaaaaag gaaaaatgcg
     9481 tggccaagta tcaaatggca tgatttgctc attacaagaa attggctatt cagatagtgt
     9541 tataccgaaa gaatacgctg aaggtatcta ttatatgcca caagaagctg taaatggcga
     9601 tccagttttt ccttatttgg atatggatga ttcgattatt gaattatcta tcacaccgaa
     9661 tcgtgcggat gcattgtcaa tgcgtggcgt agcttatgaa gttggggcaa tttatcgtca
     9721 gactcctaca ttcgatgacg aaaagctggt agaatcctca cgtcctgcta gcgataaaat
     9781 tcaagtggaa gttgaagata aagaagcagt tcctgcttat caaatcagaa tcattgaagg
     9841 agtaagaatc cagccaagtc ctcaatggct gcaaaatctt ttgatgaacg aaggcattcg
     9901 tccaatcaac aatgtagtag atgtgacgaa ctatattttg cttttattcg gacaaccact
     9961 acatgcattt gactacgaca aattgaaatc aaaagagatc tttgtccgcc gtgcaaaaga
    10021 aaatgaaaca ttagtaacac tagatggaga atctcgggaa ttgacaactg acaatctcgt
    10081 cattactaac ggcaaagaac cagttgcttt agctggtgtc atgggagggt tagactcaga
    10141 gatcacgaat gaaacaacaa cagttgcact agaatctgct ttatttgatc caatttcgat
    10201 tcgtcgtaca gcaaaacaat ttaatttgcg tagtgaatcc tctgcacgtt ttgaaaaagg
    10261 aatcaaccat gcgactgttg gagaagccag tgaggttgct gcagcaatga tcgtttctct
    10321 tgcgggtggg gaagttctac aaggcgcggt caaaggctca gagatcaaag cagaagacgt
    10381 atcagtgaag atttcacttg aacgcatcaa tcaatattta ggaacatctc ttgtcgttaa
    10441 agaagtaaat gagatttttg aagcacttgg atttagctat gaagaaaagg agtatgttta
    10501 ttccgtagta gtaccaccaa gacgctggga tatccaaatc gaagcagata ttatcgaaga
    10561 agttgctcgt atttatggat acgaccagtt gccatctaca cttccaagtg gagaaacggt
    10621 tgctggaagc ttgtcaaaag aacaagaaac tactcgtaaa attcgtacaa tctttgaagg
    10681 aagcggattg agcgaagcaa tcagttatgc attgacaaca gaagaaaaat caagacagtt
    10741 tgttataaat gaaagtaata ttacacgttt ggactggcca atgagcgaag aacgttctgt
    10801 attacgaatg aatttgatca gcggactatt agatgatatc agttataaca ctgcccgaaa
    10861 aaatacagag attgctttat atgaaattgg tcgggtgttc ttccaagaaa atgatccacg
    10921 tacacatctg ccgcaagaag taaaacatgc agcaatcgct atttctggta tttgggaaga
    10981 aaaagattgg cagacgaaag ctcaaaatgt agactttttc accatcaagg gtcttttaga
    11041 aaacttattt gaacaactag gcatcactga agatgtccat tatcaaacag tgacagaaat
    11101 gaaggaaatg catcctggaa gaactgctgc gatttacctt ggcgaaaaat ttattggttt
    11161 tgtcggtcaa gtgcatccaa cgattacaaa aagttatgat attccagaaa catatgttgc
    11221 tgagtttgat ttatctgcta tagtggaagc tgctcaaaaa gggatcgttt tcgaagcagt
    11281 gacaaaattc ccagcagtca gccgggacat cgctttgctt gtaaaagaaa ctgtgacaaa
    11341 tcaggaatta acagaagtta tccgttctgc tgccggaaga tttttaacag atatccaatt
    11401 atttgatgtc tatcaaggag aaaacattga aaaaggacat aaatccatgg cgtatagcct
    11461 gacattcgtc aacccagaag caacattgac agatgaagaa atcaataaag caatggaaaa
    11521 agtcgaaaaa gcgttggtag aaaacctaga ggcaagtatt cgctaagaag gaaaatatag
    11581 taagtaacga tcaagtagct tttgaagacc atacatttga aaaaaagatg tgacacaact
    11641 tcgtcacatc tctttttttg attttaaaaa aggaatagca cagagacgag ataaaaaaac
    11701 aggcctttac agcctgcttt tttagagtaa atgaattaat ggaagtaatt gattcccatt
    11761 gctgatttta cttcagaaag tgtttgtgca gctactttag cagcttcttc actaccttta
    11821 cgtaagattt ccataacagc tgaaggatct ttttccaatt cttctcggcg cgcacggatc
    11881 ggtgcaaatt cagcttccaa tacatcaatg aggtatcgtt tgatctttac atcaccaagt
    11941 ccgccgcggc ggtaatgagc tttcatttct tcaattgctt ctttatctgt tccgaataca
    12001 tccaagtagg tgaagaccat attcccttct acttgtccag gatcttgtac atggatgtgg
    12061 tttggatcag tatacatact catgactttt ttctgtaaga catcggcagg atcggagata
    12121 taaataccgt ttcctaacga tttactcatt ttgccatttc catcgattcc aggtaatcta
    12181 cccattcctt ttggtgggaa tacgcctttt ggttccacca agacttcacc atatgtatga
    12241 ttgaagcttt gtacgatttc ctgtgtctgc tctagcattg gtttttgatc ctcaccaaca
    12301 ggtacaagat tcgctttgaa tgcagtgata tctgctgctt gagaaacagg atagataaag
    12361 aatcctgtag gcacgctttc accgaatttt ttttgttcga tttctgtttt gacagtcgga
    12421 ttgcgccgaa cgcgtcctac gctgactaag ttcaaataat acatggttaa ttcagctaat
    12481 tccggaattt gtgattggat aaataaagta gattttgcag gatctaatcc cacggctaaa
    12541 tagtctaaag caacttgcaa gacatttgat gaaaccttct caggattttt agcattatct
    12601 gtcagtgctt gcatgtcagc aatcatgaca aacagttggt tgtcttcatc tgcctgcata
    12661 gccactcggg tttttagaga accgacgtaa tgccctaaat gcagcttccc agttggtcga
    12721 tctcccgtta aaataatatt tttcaaaaaa gacacctctt tacgatttgt aagtctgagc
    12781 acttaatatc ttaccgtttt tcctgaaaag aaacaacagt tcctaaggaa aaaacaagga
    12841 aagaggataa aataactaat agattagaaa tggaagaaat atttatggga gaataaacgt
    12901 tctaagaaac tttacaaatt actaaactat cagatataat gtattttatt atacatcttt
    12961 aaaagaaatc ttactaaata ataatgggag tgtaattcta tggcaatgat taaatttgat
    13021 cacgtggata aatattatgg caagttccac gcattaaaaa atatcaatct cgaattcgag
    13081 aaaggagaag ttgtcgttgt cattggtcct tctggttcag gaaagagcac gatgcttcgc
    13141 tgtatcaatg gattagagac gatctcttca ggtaagctgc tgattaatga tactgacttg
    13201 catgataaaa agacaaaact gacagaagtc cgaaaaaaca tcggcatggt tttccaacat
    13261 ttcaatttgt acccaaataa aactgttctg gaaaacatca aattagcacc aatcaaagtg
    13321 ttgaagcaag accaggcaac tgcagtcaaa aacgcggaga aatttttgaa aactgtcaat
    13381 atgttagata agaaagattc ttatccttcc atgttatccg ggggacaaca gcaacgggtg
    13441 gcaatcgcca gaggtttagc gatgaatcct gagatgctgc tgtttgatga acctacttct
    13501 gctttggatc cggaaatgat tggtgatgta ttagacgtta tgaaaaaatt ggctcatgac
    13561 ggtatgtcaa tgattgtcgt gacacatgaa atgggctttg ccaaagaagt ggcggatcgt
    13621 gttatcttca tggctgatgg agaagtattg gaagacagca gaaatgtgag agactttttc
    13681 gaaaatccaa atgaagtccg agcacagcaa tttatcagta aagttatcaa tcattgatag
    13741 gaggaaaaag atgcgcaggt taaaaaaaat acgcctgatt ttccttttag gcattgcttt
    13801 tcttgcacta gccggatgta aaggaaatgg gttggcaaat gaagatattt tagagcgaag
    13861 caaatctacc aatgaaatca tctggggtgt caagtatgat actcgattat ttggaatgat
    13921 ggatattgag agcagaactg tacaaggatt tgatgtagat attgcaaaag caatcactaa
    13981 aaagatcctt ggtgacaatg gaaaaacaga atttgttgaa gtcacttcta aaactcggat
    14041 tccattattg aaaaacggaa atatcgatgc aattattgca acgatgacga tcacagatga
    14101 acgaaaaaaa caggttgact tttcagatgt ctatttcgat gctggccaag ctttgttggt
    14161 aaagaaagga agccaaatca aaagtgtaga tgatttgaat gcttctacaa cggttctagc
    14221 agtcaaaggt tctacatcgg ctgcgaatat ccgacagcat gcaccagatg caaaaatttt
    14281 agaattagaa aactatgcag aagcatttac tgctttacaa tctggtcagg gagacgcgat
    14341 gactacggat aatgcgattt tgttagggat tgcagatgaa aatccagagt atgagttagt
    14401 tggtggaacg tttactaatg agccttacgg gattgcgatc aacaaagggc aagaaaactt
    14461 cttgaaagct gttaaccaag cgctagagga aatgcatgca gatggcacat acgataaaat
    14521 ctatcaaaaa tggtttccaa atgaaactga aggaaaagta gagtaagggg gagaggcaat
    14581 gagcgaattg atacaaactt atggaccaat ctttgtcgac gggttcaaga tcacattgtt
    14641 ttcgagtgtc ttagcacttg tgtttagttt gattatcgga acgttaatgg caatcttcca
    14701 attatcaaag aataaagtag tgagcgcctt agcaaaggca tatgtagaat tttttagaaa
    14761 cattccatta ttaatcatcg tgatgttttt ttatgttgtc gtaccgattt attggtttaa
    14821 tctagatgga ttccaagcag gtacgattgg attaacgatc tatacatctg cttttattgc
    14881 tgaaacagta cgttcaggga ttcaaaccgt accaaaaggt caaacagaag caggactatc
    14941 ttctggtttt acctattcag aaatcatgcg ttatattgtt ttaccgcaag cattcaaaat
    15001 cgttatcccg ccattaggca accaatttat caacctcgta aaaaactcat ctattttagc
    15061 aatcgtagct ggtttagatt tgatgtatca aggagacttg atcgctagtg aaacattcaa
    15121 taccttcgat acgtatatca tcgttgggtt attttactta gttatcacgc taccgttatc
    15181 ttacttaatg gtttatcttg agaaaaaatg ggcggttcgt tcatagaagg aggggaaata
    15241 catggatttt agcggcgctt tttcatggat gaatatccgt tttttattgg aaggattaaa
    15301 ggttactgtt gaagtttcat tgttttcgat catttttagt ttcttgatcg gtggtctgac
    15361 aggtgtgatt cgttttgcga atatcccttt cttttccaaa atccttggtg tagtgattga
    15421 tattatacgg aatcttccat tattattgat cattttcttc acttactttg ccttacctca
    15481 aatcggcatt cagatgaaca tcttctggtc cgctgtcgca gcattaacga tttttgagtc
    15541 agctatgttg tcggaaattt tccgagctgg actcaatgct gttccaaaag gacagatgga
    15601 agcaggatta tcaacgggac tgacttatgt agaatcgatg cgaacgatcg tgttaccgca
    15661 agcatttaaa tcaatgatcc cggcaattgt gagccaattg atttcactga tcaaagatac
    15721 ttcattagca gtaatcattt ctttacctga actttctcat caggcacgta ttatttatgg
    15781 acagaacacc aattatgtgc ttccgatgtt catcatcatg acacttatgt acttcgtggt
    15841 atgttacgcg ctttctctgc tttccggtta cttggaaaag agacggtaca gttattaata
    15901 gatgattctt ttagcggttg tggcaaacta aagtcacaac cgcttttcct ctttccattc
    15961 gaaaaaaaga gatttcgatt gttttaagca agtaagagca cttgaaaatt cacgctaaaa
    16021 agtacacaat agggaggata gaatttattg aaaaaaagga tgatacgatt gacagataat
    16081 cgccctatcg gatttattga ttcaggtgtc ggcggcttga ctgtagtaaa agaagccctg
    16141 aaacaattac cgaatgaaaa tattttattt gtaggagaca cagcacgctg cccatatggc
    16201 cctagacccg cggaacaggt aatccagtat acttgggaaa tgacggatta tctggtggag
    16261 caaggaatca agatgctggt gatcgcctgc aataccgcaa ctgcggtggc tttagaagaa
    16321 atcaaagctg ctctttctat tccagtcatc ggtgtgatcc ttcccggtac tagagcggca
    16381 gtaaaaaaaa cacaaaataa acaagttggc attatcggta cgattggtac ggtaaaaagt
    16441 caagcttatg aaaaagcact gaaagagaaa gtaccagaat tgactgtgac aagtcttgct
    16501 tgtcccaaat ttgtttcagt tgtcgaaagt aatgaatacc attcatcggt ggcgaaaaaa
    16561 attgtggcag aaacattagc tcctttaacc actaaaaaaa tcgatacatt gattttggga
    16621 tgcacccatt atccattatt acgccccatc attcaaaatg taatgggaga aaatgttcaa
    16681 ctgatcgatt ctggagcaga aacagtaggt gaagtatcta tgctgttaga ttatttcaat
    16741 ttgagcaatt caccgcaaaa tggtcggaca ttatgccagt tttatacaac tggctctgcc
    16801 aaacttttcg aggaaatagc tgaagactgg cttggaatcg gacacttaaa tgtagaacat
    16861 atcgaattgg gaggaaaata aggatgcgtc atgacgggag aaaagcccaa gagcttcgca
    16921 aactggagct tcaaacaaat gttttcaaat atccagaagg atcggtagtg atccgttttg
    16981 gtgatacaac agttatttgt tctgcaacaa tagaaaattc tgttccccct tttttgagag
    17041 aaacaggaac aggatgggtg acagcagaat atagtatgct gcctcgagca acgagtacga
    17101 gaaatcgtag agaaagcagc aaagggaaac tttctggtag aacaatggaa atccagcgtc
    17161 ttattggtcg ttccttgcgt gcagtagtag acttggaaaa attaggagaa cgtagtatca
    17221 tcgtagactg cgatgtgatc caagcggatg gcggtactag aacagcaagt atcactgggg
    17281 catttgtggc attgaaatta gccattgaaa aacttttaag agaaaaagaa ttgtcagaag
    17341 atccaataaa agaacatctt gctgcagtta gcgtaggaat cttaccagat gggacttgcg
    17401 tcacagattt ggattaccaa gaagattccg cagcacttgt agacatgaat cttgtaatga
    17461 cagaatctgg aaaattcgtg gagattcaag gaactggaga agaagcaacg tttgatggtg
    17521 aacagttgaa tgaaatgctc ttttttggaa aaaacgcgat tgaagatttg atcaaagagc
    17581 aaaaacatgc tttgctaaca gagtttgctc aaaatgatga gcggatagaa gaaacaaaaa
    17641 caatcatcat tgcgacaaga aatccaggaa aagcagaaga gtttcgaaat atgttcaaag
    17701 aagcgggata tcatgtaaaa acattacttg actatcctga attgccagat gtggaagaaa
    17761 ctggcagcac atttgaagaa aatgcccgat taaaagcaga aacgattgca caattgcttg
    17821 atcagccagt cttagcagat gattcaggtt tgaaagtgga tgctttaggc ggtatgccag
    17881 gaatctattc tgcacgtttt gcaggggaac aaaaaagtga tgcggggaat aacgccaaat
    17941 tattatatga attgacagat gtgccggatg aaaaaagaac agcacaattc cattgtacgt
    18001 tagtattcgc ggcacccaaa aaagacagcc ttgtagtaga agcggaatgg cccggtcgag
    18061 tagcccgtat accatctggc gaaaatggct ttgggtatga tcctttattt atcccagaag
    18121 gtaagaaaca aacagcagca gaattatcga gcgaagaaaa aaacaagatc agtcatcggg
    18181 ctcaagcaat gaaaaaattg agtgccgagt ggaaacaatg gctggaagga gagagataga
    18241 tgcggtattt agtagtaagt gataatcatg gagacagaga tatcctcgtt gacttagtag
    18301 atcgttatcg ttctgaagtg gatatctttt tccattgtgg tgattcggaa ttagaagctt
    18361 ctgacccatt atgggataca ttccaagtgg ttcaaggtaa ttgtgattat ggaccaggat
    18421 tcgaacagaa aaaagtgatc caaactggtc aggatacagt attcatgact cacggtcatt
    18481 tatcgaatgt ccgatttggt ttgacacagt tagcaatcga agctcgagct gcaactgccg
    18541 atatggtgtt ttttggtcat acccatcaaa taggctgtga gatggatcag aaaatcctgt
    18601 ttttgaatcc tggtagcatt tctcagccta gaggcccgat ccaaatccca tcatatgctc
    18661 ttatcgaaag tttagatgac caaatttctg ttcaatacta taaccgtgcc catcaggcag
    18721 ttgaagagtt ggccttttct ttttcgagag agcgctgaat aaaaaaactc taatttttaa
    18781 taaaaataag ctttatttta tgaattttgc atccggtttc ggtaaaatag tagtgttgaa
    18841 tgaaatagaa atggaggcca tcagatgatt ggacctatag taagagaaat gctgctggaa
    18901 aatcaggaaa cgtttttgat tcctgcagaa aatgtggcaa atgttatgta tcatcatcca
    18961 ttgtcccatg gtttacttgt gttatcgaag gtcggttatt ccaaaattcc tgtattagga
    19021 aaagatgatc actttgtagg actagtaagt ttatcagatg tggtcaataa aatgattgac
    19081 ttgcaaacaa tcagcatgga cccgttagaa ggactaacgg ttgctgatgt gacagaaaca
    19141 gatgttccga caattgatga aaattgggaa ctagaagaag tactccattt gttagtcgat
    19201 tcgccttttt taccggttgt cacagaagat cggattttca aaggaatcat tacaagaaaa
    19261 gagctgctta aagccgtaaa cttcatggtg catgaattag aacgtcgtaa tatattgtct
    19321 ccgaaaatcg aagaattaaa agaacaaatc gatatcgtga gctaaagaac gagaaactaa
    19381 aagataatca aaaaataagt agaaaaaaca gcgtaccagt tttgacttta tcttgaaaaa
    19441 gaggctggga caaaagcgtt taactccaag aaataagaag gaattcacga aaattgcttt
    19501 tcaaattttt gtgaatttca gcttatttcc gaaggagttg cttctgcttc cgccgtttat
    19561 acgtgtttag agcgtgggac aaaagtgttc tttacttttg tcccacgctc tttttagtga
    19621 gattcgttgc atcgtttgtc caaattattt tccgacagca attggactgt tcggaatcgt
    19681 gaaaccagaa gctgtcaatt ctttgacata ggagctcaag aactcttctt ttaaggcata
    19741 ttgttttccg ttcaatgcgt agcagaccgt acgaatcgca aaattgctgt ttccaagatc
    19801 cactaatcca aagatagaag gttcagtttg gagctcttct tggtattttt cagctaatgt
    19861 acgatttact cgatcaatga tctcatagat tttgtcatat ccttcttcag gaacaatccg
    19921 aatatctaaa agtacttgca tatttgcacg agagagattg ctgatcgtgg tgatgtttcg
    19981 attcgggatg aaatggacag taccatccgt tgctttcagc tgaagggtac ggataccaac
    20041 agatacgacg gttccttcaa tcgttaagtt tgctagcttg atgtagtccc caacatccat
    20101 ctgttgttcc atgataataa aaaatccggt aataacatca ctcatgaacc cttgggcacc
    20161 aagaccgatt gccacacctg caattccggc accagccaaa agtgatccga tcggcacacc
    20221 gatagcagat aaaattgcat agataaaaaa gaaaccaagg gtataatgaa aaatagtatt
    20281 taaaagacta tgtaaagtct ttaagcggct ctcgtttaga atggcttttt tagaatactg
    20341 attaaaagac ttatcaatca gatattttcc cacacggtga agaatccaaa aaaggacgat
    20401 caaaaaaatc agatataaac ttttttggat aaaggcagcc aaaatggcgt cccaatcaat
    20461 cccgtcccaa aaccgctgaa acgcatttac tttgctgaca gcttgatctg tcaatgtctc
    20521 gccagaagag tctgtcgtac cggcagttgc taataatgaa aacataaaat cctcttttct
    20581 aaaatttgat tctttctgta ttgtaccaag aagttttcaa aaaaggaaat agcaaaaata
    20641 actataatga aggaaaaaag ctgaaaataa aatgacaaat aatatggata atgataaaat
    20701 gtggagaaaa taacgaaggt ggtaagtata tgcaaatcga ttggaacaat ctaggttttt
    20761 cttatatcaa aactccttgg cgttttattg ccaaatggaa agacggacaa tgggaagaag
    20821 gagaattgac agaagataat tacctgacga ttcatgaagg ctctccagcg cttcattatg
    20881 gtcagcaatg ttttgaaggt ctaaaagctt atcgtcgaaa agacggcaag gtcaatctct
    20941 ttcgacctga acaaaatagt cgacggatga atcaaagtgc tagacgattg ctgatgccaa
    21001 tggtccctga aaagatgttc gttgatgctg tcaagcaagt ggtaaaagca aatgaagatt
    21061 ttgtgccgcc ctatggaaca ggcgctacat tatacttgcg ccctttactg attggggtcg
    21121 gtgaaaacat tggtgtccat ccagctccag aatatttatt tgtcgtcttc tgtacgccag
    21181 taggagcgta tttcaaaggt ggactaaaac caacgaattt tattgtatct gattacgatc
    21241 gtgctgctcc taaggggact ggtgcagcaa aagtcggcgg aaattacgca gcgagtttac
    21301 ttccaggaac agaagcaaaa gaactggatt actcagattg tgtttatctt gatccagcca
    21361 cacatacgaa aatcgaagaa gtaggtgcag caaatttctt tggaattaca aaagacggaa
    21421 aattcatcac tccgcaatcg ccctctatct taccaagtat cacgaaatat tccttgttgc
    21481 agttagctga agaacgacta ggactgcaag caattgaagg agatattttc attgatgatc
    21541 tagatcaatt tgcagaagct ggtgcatgtg gtacagcggc tgttatttct ccaatcggtg
    21601 ggatttatca caataacaat cttcatgtat tttatagtga aacggaagta ggaccagtaa
    21661 caaaacggtt gtatgatgaa ttgacaggta ttcaatttgg agacatagaa gcaccagaag
    21721 gctggattga agtcgtggaa taatttcaaa gtaatgagtt gaaaaaaacg taaaaagaaa
    21781 aaatcaggaa gagactttaa aaagtttttt cctgattttt atatgtttta ctttaaagaa
    21841 tgcaacaaaa gtttatgaaa gatggacacc tttgtcaacg tagataatat caccgatgat
    21901 tccgctggaa agtgggctga ttaagaaagc acaagcatta ccgacttctt cgatcgtcac
    21961 accttgctga tcaggcgtac gagattcaga taactggatc agtttttgat agtctttaac
    22021 acctgtgaca gctaatgttt tgattgcccc agctgagatt ccattgaccc gaattccttt
    22081 aggcgagaac tcagctgcca aataacggat agcagcttca agagaagctt tcgcaatacc
    22141 catcatgtta taattcggga ttgcgcgttc tgaacctaga taagtcatgc taacgatccc
    22201 gctgtttggt gcaagaaacg gttgtgcgta ttttgataca gcaattaaag agtagctgct
    22261 gatatcttga gctaattgat agccgtctct ggaaatatca gacacatttc cggacaattc
    22321 ttctttgttg gcgtaagcaa ctgcatggac aaggccgtgg atctccccag catattcacc
    22381 gatggcattc attgcttttt cgatgctttc gtcgctagaa acatcacatt ctacagtgaa
    22441 tgcttcttcg ccgaccaatt tgactaatga ttttttcatt cgttcatttt ggtaagtata
    22501 gataatttct gccccttgtt tcaacattgc ttcagcacaa ccccaagcaa tgcttttttt
    22561 gttagcgacg cccataatga cgattttttt tccttctaga aatgacataa tgactctcct
    22621 ttgtttctga taaaaatttg atagtcgtaa aaaaatagag aataatagct aaaataactt
    22681 cttgatttct taattattag tcaaaatgtt ttgattgtca aactattttt tcttaaatta
    22741 aaaaaagatt gctaaatatt taggaatatg atactttgat agtcaaagta atgaaaatgg
    22801 caattgcctg aaagaaggag tttaattatg tcttacgtat tatctgctac tgaagtaatg
    22861 gaattgattc ctaatcgtta tcctatttgt tatattgatt atgtagatac tttagaacca
    22921 ggaaaaaaga tcgttgcgac aaaaaatgta acaatcaatg aagatttttt ccaaggtcac
    22981 tttcctgaca atccagtgat gccgggtgcg ttgattctag aaacactggc acaagcaggt
    23041 tcgatactta tcctaaaatc agaagagttt ctaggaaaaa gagcttatat cggtgggatc
    23101 aataaggcca aattccgtca aaaagttgtt ccaggagatg tgctgaaatg tcattttgaa
    23161 atcaccaaaa tgaagggatc agtcggtaca gcgttatctg cagcatatgt agatgataaa
    23221 aaagtctgtg agtgcgagtt cacgttcatc gtcaaccaaa atgcttgact ataaaacctg
    23281 atatagcaaa acaaaaggaa gcttacttgc tttttatgta aaaagaagac gcctgaatcc
    23341 tagcatacgt tgtcatctat gcaggattca gacgttattt ttttattttt gttttgcttc
    23401 ttgcagtata ttgattttat cgatttttac ttcttctttt ggtttgtctg aactatctgt
    23461 ttcgatattt gcaatagcat ccacgacatc cattccttca atcacttggc cgaagacagt
    23521 ataatggcca tctagtgaag gataaccgcc gtttttataa gcgtcgatga ttgcttgagg
    23581 atagtcgtct tttaaaagac catcatgcat atcatctgta ttttgaacga taaagaactg
    23641 gctgccgtta ctgttaggat cttgtgagcg agccatagat aacgcacctc ttatattata
    23701 aaggtggttg ttgatctctg tttcaaatcc ttctccccaa atgctttccc ctccagtgcc
    23761 atcgccttta ggatcgccac cttggatcat gaaatctttg atgacgcgat ggaaagtcag
    23821 tccgttgtag taaccgtctt ttgcatgtgt cataaagttt tcaaccgctt ttggtgcgtg
    23881 ttcgggaaat aatttgattt tgattgtgcc tttattcgtc accatttcga ccagatcttc
    23941 attttcggcg acatctttcg tcaattgtgg taattccaat gcgttcaaat cgacgctact
    24001 ggcagtcgtg ctttccgcag tagatgtact ttttgaactt tctgtagacg aactagaaga
    24061 agtcgtgttg tttgcagaac aaccagcagc aaacagcaat gctgctgcta aactaatact
    24121 taaaataact ttatttttct tcataactgt tcctttccaa tttactattt tgttatcttt
    24181 aatgataaca aaatagaggg aaaataccta cttaaattat tggaatttat catttatttt
    24241 aaaagaaaaa gaaaaggtgt agaccattta ctttgtgttt gctataatgt aagtggatac
    24301 aaaaacgaaa agatgcagga ggaaatggta atatgggaaa attaggaatc tcgatttatc
    24361 cagaacggtc aacatttgaa aaggacaaag cgtacttgga tttagctcat aagtatgggt
    24421 ataaacgggt ttttaccagt ttgctgcaga tcaatgatga taaagaaaaa gttctttcag
    24481 aatttaaaga agtagttgat tacgcgaata gcctaggcat ggaagtaatg gttgatatca
    24541 atccagcgtt atttgaacag ttggagattt cttacgatga cttatctttt ttccataaga
    24601 tgggtgccta cggggtccgc ttagatatcg gattcacagg agcagaagaa gcaaaaatga
    24661 cgcgcaatcc ttttggtatc aaaatcgaga tcaatatgag ttcgggcacg aattacgttg
    24721 acaacatcat gagttattcg ccaaacacgg ataatttatt aggttctcat aatttttatc
    24781 ctcatcgata ctctggttta ggatacgaac actttgtttt ttgttcagaa aaattcagaa
    24841 aatataattt gaatacgatg gcattcgtca attcgcaatc tgctgagttt ggcccttggc
    24901 ctacgcaaga tggcttgtgc acgctggaag accaccgaga tcttgaaatt gcgacacaag
    24961 tgaagcattt aatccttaca ggattgattg atgatatttc tattggaaat gcgtacgcaa
    25021 gtgaagaaga attgaaagaa atggcagaag catttaacgc ggattatcca acattgaaag
    25081 tagatacgga agaaggtatt acagaaaacg aacgaatctg cttgtttgat aaccttcata
    25141 gttatcgagg ggatcgctca gaatatatcc ttcgttcaac gatgactcgt gtatactaca
    25201 aagataaaga tttcccgcct cataatacgc gagatatgca tcatggggat gtactgattg
    25261 acaatgaagg gtacggtcaa tacaaaggag aaacacaaat cgccttgaag gatatgaaaa
    25321 acgacggacg tgtcaatgtt gtaggaagaa tctccgatga cgaattattc ctccttgact
    25381 ttttgaaacc atggtcaagc tttaaattaa ttgaaaataa aaaatagata attagttatt
    25441 gcagaaataa tccgctagaa atagccagtt atttctgctt ttttattttg tttttttata
    25501 ggttatgatt tgtcaaatta agctagaata aaatgcttct tgtacatagc tcaaaaatat
    25561 ttcgattggt gttctgtaat tcaatgattt tcttggaata tgattacgtt gattgctgac
    25621 actggaaata aatgtctgat tcacttttct aaaatccatt gatttcggca gtccattacg
    25681 acgcagaatc ccgttagaat tctcgtttaa tgggcgttga gaaggtgttc cagggtccgc
    25741 aaaatatata tcaatatcat gttggttact aatggctttc cagttagaaa attcttttcc
    25801 acagtcaaac gtaatagatt taaagaagtt tttagggaag cgagaaaacc attgattaag
    25861 ggcagtttca atatctaatg ccttacggcc gttgggttta atcgtgataa tgactttaga
    25921 taatctttca actaaagtaa tgacggcact tttatgatga atgccaacga tcgtatcacc
//
LOCUS       DARBET010000032        25929 bp    DNA     linear   BCT 07-NOV-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4027
            SAMEA114569841-rid20112183.denovo.032, whole genome shotgun
            sequence.
ACCESSION   DARBET010000032 DARBET010000000
VERSION     DARBET010000032.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA114569841
            Sequence Read Archive: ERR12203666
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 25929)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 25929)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001972618.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001972618.1
            Genome Coverage       :: 132x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/07/2023 13:18:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,041
            CDSs (total)                      :: 2,975
            Genes (coding)                    :: 2,908
            CDSs (with protein)               :: 2,908
            Genes (RNA)                       :: 66
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 67
            CDSs (without protein)            :: 67
            Pseudo Genes (ambiguous residues) :: 0 of 67
            Pseudo Genes (frameshifted)       :: 26 of 67
            Pseudo Genes (incomplete)         :: 28 of 67
            Pseudo Genes (internal stop)      :: 20 of 67
            Pseudo Genes (multiple problems)  :: 6 of 67
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..25929
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA114569841-rid20112183.denovo.032"
                     /isolate="VRE4027"
                     /db_xref="taxon:1352"
                     /country="Denmark"
     gene            154..576
                     /locus_tag="SCJ86_001508"
     CDS             154..576
                     /locus_tag="SCJ86_001508"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288984.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HEG1169051.1"
                     /translation="MQTFLTKVSQKKIKMIHLLLETERWCSIEELQNELKVSSKSILN
                     YLTDLEELFQQYPDKVILKNENNRRFSMEKQENFPIYIIYLHFYRKSYNYHLIEFMYQ
                     HPEKNLEDYADAQYTSVSTVFRYAKLLVKYFEMTDVKE"
     gene            complement(742..1935)
                     /locus_tag="SCJ86_001509"
     CDS             complement(742..1935)
                     /locus_tag="SCJ86_001509"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEG1169052.1"
                     /translation="MTTYQRKVLVSTSAGIALENMDIMFLAFSLSSMITSFHISGTQA
                     GFISTITNLGMLIGGIFFGIIADKYGRVKVFSQTVLLFSIASLLMYFASNIYLVYLFR
                     FIAGVGAGGEYGACMSLISESFSKKQIGRASSVAGIGAQVGAALAAILAAVIIPTFGW
                     KMLYVVGVLPVLMVLFIRRGLTEPKEFKSVQSAEKKTKLSRLFESKHLAWQTCGLSIM
                     VTVQIAGYFGLMNWLPSIMQNRLGLSVSGSSLWMVSTILGMSLGMMTFGIIMDKLGPK
                     LAFSLFLICSSLSVYLLVLAHSQWSLVAAAVIVGYFINGMYGGYGAVISSLYPTEIRA
                     TANNFVMNLGRAVGGFSSIVIGFLMDHYSLTAVILFLSGIYLVSLLVLTTLTGVTALR
                     ESLQH"
     gene            2159..2536
                     /locus_tag="SCJ86_001510"
     CDS             2159..2536
                     /locus_tag="SCJ86_001510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302962.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
                     /protein_id="HEG1169053.1"
                     /translation="MNIKQVSEEKGISADTLRYYERIGLIPPVNRTKGGIRDYTEEDL
                     KWVDFTICMRNAGLSIESLIEYIRLSSEGEATVFERRKLLIEESELLTKKIAEMQECL
                     ERLQGKIKKYDRVLSRNKMECIK"
     gene            2524..2862
                     /locus_tag="SCJ86_001511"
                     /pseudo
     CDS             2524..2862
                     /locus_tag="SCJ86_001511"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321036.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
     gene            2996..3439
                     /locus_tag="SCJ86_001512"
     CDS             2996..3439
                     /locus_tag="SCJ86_001512"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631212.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator
                     substrate-binding protein"
                     /protein_id="HEG1169054.1"
                     /translation="MLDPVDTEKYETILLPRKEKWGLLILENSVLAKKDVITKEDFAN
                     VPLLISKRPEIQRMFADWLGIKKEQLLITGTYNLIFNVFALIENQVGAALVIEGAVSH
                     HSKRVTFVPLDPMIETNCMLVWKKNRILSPVVNELIHRFKEAFEQ"
     gene            3700..4515
                     /locus_tag="SCJ86_001513"
     CDS             3700..4515
                     /locus_tag="SCJ86_001513"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010731016.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HEG1169055.1"
                     /translation="MPFIQWLGMILLIVLALLTIIPFLWMVSASFKANNEVFTIPIQW
                     IPKTWHLENYLVIWSRIPLVTFFKNTAFLSVVITLIQLFTSSFAAYGFSKLNFKGRDI
                     LFLAYIGTIAVPWQSYMIPQFIMMRKVGLTDTLWSLVLLQAFNAFGVFLLKQYYSSIP
                     DSLCESARIDGLSEWGIYRKIILPLTKPAIASLTIITFVNTWNDYMGPFIYLSSTENK
                     TIQLGLKMFIGLFDAEYALIMAASVVSVLPVAFIFLAMQKYFVEGIASSGMKE"
     gene            4525..5187
                     /locus_tag="SCJ86_001514"
     CDS             4525..5187
                     /locus_tag="SCJ86_001514"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF624 domain-containing protein"
                     /protein_id="HEG1169056.1"
                     /translation="MKEQRFDKNVYMEFFYWLYQSLASSLCFWCVNILYIAAAFLLSI
                     DGRNVFSFIFSLLFIGPGCIVMIGMTEELAKENSFSPVRLFIKMFRQFWLKGFFYWLF
                     AWIVSVIMIFDCFFFIRFSYGKWLIPLFVLLACLSVSFSTNCWYFQVRNPASKPNQVL
                     RIAFYYTLKKWYVSLLDFLLLTSLFLFFFVKPQWCILLGPSIVFGLIYFNNRKLMRTM
                     DL"
     gene            5395..7059
                     /gene="alsS"
                     /locus_tag="SCJ86_001515"
     CDS             5395..7059
                     /gene="alsS"
                     /locus_tag="SCJ86_001515"
                     /EC_number="2.2.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002352535.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetolactate synthase AlsS"
                     /protein_id="HEG1169057.1"
                     /translation="MAEKMKQGSKSVVESLINHHVDYVFGIPGAKIDGVFNELEDQGP
                     ELIVTRHEQNAAFMAQAIGRITGEPGVVIATSGPGASNLATGLVTATAEGDPVLAIAG
                     QVKRSDLLKLTHQSMDNAALFQPITKYSAEIQDPETISEVIANAYRMAKSSKKGASFI
                     SIPQDVVDAPVQGNVIKPLSDPKLGSASADDIRYLAERIREAKLPVLLVGMRGSSEKE
                     TLAIRQLVEKTALPVVETFQAAGVISRELEAHFFGRVGLFRNQPGDMLLKRSDLVIAI
                     GYDPIEYEARNWNAEKDARIIVIDEAPAEIDPFMQPERELIGDISATLDLLTGSLEPQ
                     QVSEDAKEYLASLQAKLTERDIVQSKGEAGILHPLEVINTLQSKVTDDMTVTVDVGSH
                     YIWMARHFRSYEPRHLLFSNGMQTLGVALPWAISAALVRPNTQIVSVSGDGGFLFSAQ
                     DLETAVRKKLNIVHLIWNDGHYNMVEFQEKMKYQRASGVDFGPVDFVKYAEAFGAKGI
                     RATSVEELEKALEEGFATEGPVIIDIPIDYRDNEKLGETILPDQFY"
     gene            7072..7782
                     /gene="budA"
                     /locus_tag="SCJ86_001516"
     CDS             7072..7782
                     /gene="budA"
                     /locus_tag="SCJ86_001516"
                     /EC_number="4.1.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333671.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetolactate decarboxylase"
                     /protein_id="HEG1169058.1"
                     /translation="MTEKILYQHGTLGALMAGLMDGTETIAHILEKGTLGLGTLHGLD
                     GEVIFLDGVAYQGRSDGSVAQLDGSELTPYAAITDFTPDTSFSVSKTADGEQLKKDIL
                     VREAGENLFLAVKITGLFKNMHIRIMPKQEKPYRRLAKISESQPEFQQSNIQGTLVGF
                     FTPELFQGVAAAGFHLHFIDDTHTFGGHVMDFEVAEGKVEISRISSLVQHFPVDDPTF
                     VHETIDYADLAAEIQQAE"
     gene            7912..8409
                     /locus_tag="SCJ86_001517"
     CDS             7912..8409
                     /locus_tag="SCJ86_001517"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631901.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GAF domain-containing protein"
                     /protein_id="HEG1169059.1"
                     /translation="MWTEKQKKDAYEILLLQQQGLLEAEDNWLANLANSSALLNETLP
                     ETVFAGYYLFDGNELILGPFQGRVSCTRIKMGKGVCGESAEKQETLIVDDVKTHANYI
                     SCDSAAQSEIVVPMVKEGNLIGVLDIDCGVTKGYDEIDQAFLEKYVDLLLANFNINAF
                     SFINN"
     gene            8594..8854
                     /locus_tag="SCJ86_001518"
     CDS             8594..8854
                     /locus_tag="SCJ86_001518"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302583.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEG1169060.1"
                     /translation="MNNETIILGKKYICQPIGLKQKVVGEVISKLENCVVICVDEYHD
                     VDRNEILEKCGKVVAKYTHVYELETTEYLYNYSNKYEHTLVS"
     gene            complement(9216..9455)
                     /locus_tag="SCJ86_001519"
     CDS             complement(9216..9455)
                     /locus_tag="SCJ86_001519"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343604.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEG1169061.1"
                     /translation="MKRILFISLSNLLLLTACGTSASKNIPSDTQISNSIDSTISTNE
                     KNSPVETSDIKVSTSAKNRVKKLSARLLYFKQTLN"
     gene            9741..10055
                     /locus_tag="SCJ86_001520"
     CDS             9741..10055
                     /locus_tag="SCJ86_001520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331484.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEG1169062.1"
                     /translation="MDFYSNFILIIAILLLLNIWFFDKSRNAGIGFRTKRSTSSEKKW
                     VYSQTIFYGGVISISLLSSTLYSFNVIDVSMSNFISIIGILISAIITQLLLVFEEKSK
                     NN"
     gene            complement(10489..10855)
                     /gene="ssrA"
                     /locus_tag="SCJ86_001521"
     tmRNA           complement(10489..10855)
                     /gene="ssrA"
                     /locus_tag="SCJ86_001521"
                     /product="transfer-messenger RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00023"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00023"
     gene            complement(11026..11799)
                     /locus_tag="SCJ86_001522"
     CDS             complement(11026..11799)
                     /locus_tag="SCJ86_001522"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346978.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cof-type HAD-IIB family hydrolase"
                     /protein_id="HEG1169063.1"
                     /translation="MSNYRAITFFDLDGTLLDAKSKITPEVAKAMNALKENNVLPVIA
                     TGRTEAEIHSIMEDAGITSAVTMNGSFIRVDGEEIYSQTFSSEECQKMYDHVQLNGHE
                     LSYYNDQKIWCTGHSQTMINAYDFIHSDVPEIDPLGFKENTVNMLLILSQSGDEYYHE
                     HFPELTFYRNGPYSIDTVKKGISKGAGVKRLQKELQLEKVPTFGFGDGPNDFALLEAC
                     DNKIAMGNAYDGLKELSTFITKKNTEGGIVHALKYFDLI"
     gene            11943..12293
                     /locus_tag="SCJ86_001523"
     CDS             11943..12293
                     /locus_tag="SCJ86_001523"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338867.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SdpI family protein"
                     /protein_id="HEG1169064.1"
                     /translation="MIFFSIGILFLIIGSLFRILPSKGNLPFYGYHSPLAAKTDAHWR
                     LAQKTSGNWFFLMGLLMALIGYYLKTSGHTNYFLIEMLLLVFPIMPIFIMTEKKLQKY
                     DLETGGNDNEYFND"
     gene            12274..12990
                     /locus_tag="SCJ86_001524"
     CDS             12274..12990
                     /locus_tag="SCJ86_001524"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338866.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HEG1169065.1"
                     /translation="MNILMIEDNQSVSEMMQMFFLNEGWEATFKYDGKEGLDAFLENP
                     QHWDMITLDLNLPTLDGMLVGREIRKVSSTVPIIMLTARDSESDQVIGLEMGADDYVT
                     KPFSPLTLIARMKALHRRSELAEAREEAAVSDDHFDIETSHFKMNTKTREAYLDNRPI
                     EGLTPKEFDLLFTLAKKPRQVFSREQLLELVWDYQYFGDERTVDAHIKKLRQKIEKVG
                     PQVIQTVWGVGYKFDDSGVA"
     gene            12990..14438
                     /locus_tag="SCJ86_001525"
     CDS             12990..14438
                     /locus_tag="SCJ86_001525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631900.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing histidine kinase"
                     /protein_id="HEG1169066.1"
                     /translation="MRYLYQQLLAFCGMVALILLIVGVSFTQLTKQTIEENNYQQLFG
                     YAESVEKTTQTYADTLPQFSQDQAFQNALLLTEQVLTEQNVNFIFIDKNERVIYPTNT
                     ALLNFTLTADQWKNLRNGRQEKFTSNKNILGQAQATSYALVPFNLNHEFYGALVVSQP
                     ARNIDNSVRPITLNLFKGFIFSSIVAVIASYAFAAFQVKRINRLRNATKEVTNGNFDV
                     QLPVHDKDEFDELADDFNKMTNSLKESQAEIEEQENRRRQFMADASHEMRTPLTTING
                     LLEGLEYNAIPENQRENAIKLMKNETERLIRLVNENLDYEKIRTNQISMVIKKFNGTE
                     TLKNIVAQLEAKAEAAGDTLTLKAVDDIDVYADYDRFVQIMVNIIQNAIQFTENGQIT
                     ITLEKGYLETIITIEDTGIGMSEQQMKSIWDRYYKVDPSRKNTKYGESGLGLPIVQQL
                     VRLHKGKLEVESELGKGTKFAVRLPDVEIKKE"
     gene            complement(14508..15017)
                     /locus_tag="SCJ86_001526"
     CDS             complement(14508..15017)
                     /locus_tag="SCJ86_001526"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEG1169067.1"
                     /translation="MKPEYLIHAEHLTVILCPLEKQHKILEHLKKEVLVAKLDNFAII
                     QKNWPMFPYLNLKDHVLLDVPEKEIKQDRLAYQEKLDISPSLLNCAAAELTSFEKVKL
                     QLLHALLSKRNNIVIEDTFDELSVLEIQELLHLLSYLAHEENQGILLFTHDATIAQSP
                     YIDRLEPAG"
     gene            complement(15007..15732)
                     /locus_tag="SCJ86_001527"
     CDS             complement(15007..15732)
                     /locus_tag="SCJ86_001527"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293939.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEG1169068.1"
                     /translation="MKIIRYATARLIYYKKQTILYCLFSSFSAFLLIACLTLYHLQSA
                     LANQVQDRLSFIGASASTELLPALELASRFYIKGIFSLFFLFAFLFIFFFYYSVRQNK
                     QELINWRLTGLSKTKLFLFIFWQLFFPLFLCCILVLLWIVSFQSVYEALLQRINFLVL
                     KTTELPDIRDILTSVSGLTIPMDQQTFFTIDFQNDLFFTDILKGFAQTLIGLSGCCLI
                     LSLFPFTFFIHHLQSRRPGRNET"
     gene            complement(15955..18075)
                     /locus_tag="SCJ86_001528"
     CDS             complement(15955..18075)
                     /locus_tag="SCJ86_001528"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346400.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LTA synthase family protein"
                     /protein_id="HEG1169069.1"
                     /translation="MKCIKTPAFLNKRLGFFSLLVVLLWLKTIFAYLVDFHLGIESGI
                     QYFILFINPIATTLLLLGIALYIRRPRVAYITMMVIYFLLTLLVFSNVTYYREFTDFI
                     TINTILGAGKVASGLGESAIRLFRPYDVIYWIDFIILTVLLIAKKIKLDPRPIRARMA
                     FAISTLAVMIFSGNLFMAEADRPELLTRTFSRDYLVKYLGLNAFMVYDGVQTYQTNQV
                     RAEASPNDMKEVENYVKEHYAAPNDDLFGLAKGKNVIYIHLESFQQFLIDYKLTDENG
                     LQHEVTPFINSLYHSNSTFSFDNFFHQVKAGKTSDAETLMENSLFGLNQGSLFTQLGG
                     KNTFQAAPDILSQTQGYTSAVFHGNAGTFWNRNETYKNLGYDYFFDSSYYDVNDDNSF
                     QYGLHDKPFFQQSVQYLERLQQPFYSKFIAVSNHYPYSKFTNDEAGFPLANTNDETIN
                     GYFATANYLDTAVQEFFNYLKESGLYDNSVIVLYGDHYGVSNSRNKNLAELVGKTSST
                     WTNYDNAQMQRVPYMIHIPGQTQGGINHTYGGQVDALPTLLHLLGVDTKNYIQLGQDL
                     FSKDHEQLVSFRDGDFVTPKYTYYGGTIYSNETGEAITEPTEEVQNEIDSLKEKVDKQ
                     LAVSDQINNGDLLRFYTNSGLDPIDTSDYDYKNGLEKLKEAEKSLGDKSTSVFSEHNG
                     QSTVDLYKTQTYQELHGTTTSSSE"
     gene            18305..19483
                     /locus_tag="SCJ86_001529"
     CDS             18305..19483
                     /locus_tag="SCJ86_001529"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008268761.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent rRNA methyltransferase"
                     /protein_id="HEG1169070.1"
                     /translation="MKVKVKKRASNRLKKGYPLIQKEDLAHPQKETTQWVELVDSQNQ
                     FIAKGYLGKQNKGIGWVLSQQNIPFDQSFFEEFFVKSKEKRTFFFSDDSTTAFRIFNG
                     EGDGIGGLIVDYYAGYAVFSWYNETLYAHRELLIQAFREIYPEIVGGYEKIRFESVNL
                     PESQFIYGKEAPEPLIVKENGVKFAVYLNEGLMTGIFLDQKEVRGSLAEGFASGKTVL
                     NMFSYTGAFSVAAAMGGAIETTSVDLAKRSLKKTQEQFEVNGLDVDQQKIIVMDVFEY
                     FKYAKRKQFLYDMIVLDPPSFARNKKKVFRVAKDYGELVKDSLDILAEDGILIASTNA
                     ANVSIEQFQTMIEEEFHAKNVSFEQLALYRLPKDFQTTDTFLEGNYLKVFVYQIKNNH
                     "
     gene            19499..20257
                     /gene="aroD"
                     /locus_tag="SCJ86_001530"
     CDS             19499..20257
                     /gene="aroD"
                     /locus_tag="SCJ86_001530"
                     /EC_number="4.2.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288579.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I 3-dehydroquinate dehydratase"
                     /protein_id="HEG1169071.1"
                     /translation="MVWVKGLNIGSGRPKICAPLIGAVREEVLREAVLAREAGADLVE
                     WRVDHYREVFSFDEVAETLVLIHEMLDGLPLLFTFRTLAEGGEQDISIRQYRDLYEHV
                     IHTGLVDMVDIELFKVESLGKRLIEEIKSLNIPLIISSHDFKETPADPVLLYRLNVME
                     HFGADIGKLAVTPNNERDVLRLMELTRRANAFVSMPIITMSMGNLGKISRLAGGTTGS
                     VMTFGSLSSDSTSAPGQLPVKALKQIIQLLNEPL"
     gene            20280..20765
                     /gene="ybaK"
                     /locus_tag="SCJ86_001531"
     CDS             20280..20765
                     /gene="ybaK"
                     /locus_tag="SCJ86_001531"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347404.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cys-tRNA(Pro) deacylase"
                     /protein_id="HEG1169072.1"
                     /translation="MAKKKIVKTNAIRMVEQKKIPYTEHEYEWDESHLSASSVAEQLP
                     ESQSRIFKTLVAVGNVTGPLVAVIPGEAELNLKKLAKVSGNKKVEMLHLKDLEATTGY
                     IRGGCSPIGMKKLFPTYLDQIAESYEQIIVSAGRRGLQMELAPQDICALTSGQFADIK
                     Q"
     gene            complement(20836..22089)
                     /locus_tag="SCJ86_001532"
     CDS             complement(20836..22089)
                     /locus_tag="SCJ86_001532"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293934.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HEG1169073.1"
                     /translation="MEKLYDVIVVGAGTSGMMAAISAAEQGARVLLIEKNKKAGKKLL
                     MTGGGRCNVTNNRPVDDLIAHIPGNGKFLYSTFAQWNNFDIMNFFESQGVHLKEEDHG
                     RMFPVTNKSKTIIEALLNRLKELDVTLLFSTRVEKLIHKEHKIYGIRTEFEEFHAPAI
                     ILTTGGRTYPSTGSTGDGYKIVKRVGHTVTPLYATESPLISDEPYIQEKTLQGLSLQD
                     ITLRVLNKKGRVLTEHTMDLLFTHFGISGPAALRCSSFVNKELEKTGEPVTLSLDCFP
                     TQTKQELIHILTEKSKATKKNLVNAWHGILPERLLVFFLERLEMDHLTGQQASQKQIQ
                     DFVQLCKEFKLSINKTFPIEKSFVTGGGVSLKEIYPKTLESKIIDGLYFAGELLDVNG
                     YTGGFNITAAFATGHVAGMNAGQRA"
     gene            22207..23556
                     /gene="gdhA"
                     /locus_tag="SCJ86_001533"
     CDS             22207..23556
                     /gene="gdhA"
                     /locus_tag="SCJ86_001533"
                     /EC_number="1.4.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631211.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-specific glutamate dehydrogenase"
                     /protein_id="HEG1169074.1"
                     /translation="MTNATEYVQAIQEKLHQKDQGQEEFLQAIDEFMPTVMSFLDTHP
                     EYIEKNILELLTEPERVIQFRVPWQDDEGNWRVNRGYRIQYNSAIGPYKGGLRFHPSV
                     NLSILKFLAFEQIFKNSLTGLPIGGGKGGSDFDPKGKSDNEIMRFCQSFMLELSKHIG
                     PSIDVPAGDIGVGAREIGYLFGEYKRLKKYDTGVLTGKPLDFWGSKIRTEATGYGLVY
                     YVKHLLNEEKDSFNQKTVFVSGSGNVAIYAIEKVQELGGKVVTCSDSSGYIYDPEGID
                     VKLLKEVKEINRQRLTEYANQRPSAIYHAGESVWTLKEKADIALPCATQNEIDESLAE
                     ILVENGIKIVAEGANMPCTVKAVSVLHEAGVWYCPGKAANAGGVAVSALEMSQNVQRL
                     TWEREQVDEQLDQIMETIYHTCRQTAAEYGDEKNLLLGANVAGFEKVAKAMAAEGLV"
     gene            23668..25014
                     /locus_tag="SCJ86_001534"
     CDS             23668..25014
                     /locus_tag="SCJ86_001534"
                     /EC_number="5.3.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340398.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-6-phosphate isomerase"
                     /protein_id="HEG1169075.1"
                     /translation="MAHIHFDYSKVAPFVNEQELGYMQSQVTAAHNELREGTGAGSDF
                     RGWIDLPTNYDKEEFARIKEAAKKIQSDSEVLVVIGIGGSYLGARAAIDFLNHTFFNL
                     LDSNTRKAPQIFFAGNSISSTYIADLIEVIGDRDFSVNVISKSGTTTEPAIAFRVFKE
                     LLVKKYGQEEANKRIYATTDKAKGAVKVEADAEGWETFVIPDDIGGRFSVLTAVGLLP
                     IAVSGADIDALMQGAADASKAYSSDKLEENEAYQYAAMRNILYRKGKVTELLINYEPG
                     MQYFSEWWKQLYGESEGKDQKGIYPSSANFSTDLHSLGQFIQEGRRNIFETVVKVEKP
                     RKSITIPEQAEDLDGLGYLQGKEVDFVNTKAFEGTLLAHTDGDVPNLLVKIPEMDAYT
                     LGYTMYFFEIAVGISGYLNGINPFDQPGVEAYKKNMFALLGKPGFEDLAKELNERL"
     gene            complement(25322..25708)
                     /locus_tag="SCJ86_001535"
     CDS             complement(25322..25708)
                     /locus_tag="SCJ86_001535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288573.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YxeA family protein"
                     /protein_id="HEG1169076.1"
                     /translation="MKKILGLIVIILIAFIGWKGWDYYQSTYVGKDYYAVVKAPMPAE
                     TDIKADNGEVIGRGFKYNVDAYAENGEKRQLDFDVITSGDSANGSAYPEGTILQLKAS
                     EKRIIEKKVITSDKVPSSVKEKLGLS"
     gene            complement(25757..>25929)
                     /locus_tag="SCJ86_001536"
     CDS             complement(25757..>25929)
                     /locus_tag="SCJ86_001536"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611882.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="IS982 family transposase"
                     /protein_id="HEG1169077.1"
                     /translation="VETVFSSLEKLGCQNFNSRSVKGLESRFESILLAYSVLLSRAQR
                     RFEGTLRYSLGY"
BASE COUNT         8588 a         4468 c         5166 g         7707 t
ORIGIN      
        1 ataaacgatt aaatgattca tatcaataga atacttagga aaaaaagaga aataataaca
       61 ggtttatgca ctattgacgg tgtgacaata tgatatttgt atccgttttt ttcttgtatg
      121 tagtatagta aagtagatac aggaggagaa aatatgcaga cttttttaac aaaagtttct
      181 caaaagaaaa ttaaaatgat tcatttactg cttgaaacag aacgttggtg ttcaatagaa
      241 gaactacaaa acgaattgaa ggtatcaagt aagtctatct tgaattatct aacagattta
      301 gaagaattgt tccaacaata tcctgataag gtcattttga agaatgagaa taatcggcgc
      361 ttttctatgg aaaagcagga gaacttccca atctacatta tttatcttca tttctacaga
      421 aaatcatata attatcattt gattgaattc atgtatcaac atcctgagaa aaatttggaa
      481 gattatgcag acgctcaata tacgagtgta tccacagttt ttcgttatgc taagttatta
      541 gtaaagtact ttgaaatgac ggacgtcaaa gaatagtcgc cgccagatag aaggaaatta
      601 gcagttgaaa aagctgccct gcatcaagta atacgaccag ccaaacggaa acagtgtctc
      661 atgtttttga tttggctggt cttattaccg cagatgcaag ctttttccac acctctttct
      721 tgtcatttac agactcaact atcaatgctg caagctttcc cttaatgctg taacaccagt
      781 aagagttgtc aagaccagca gactgactag gtaaataccg ctcaaaaaca agattacggc
      841 agttaaacta taatgatcca tcaagaaacc gatgacgata gaagaaaaac caccaacagc
      901 tcgtccaagg ttcataacga aattattcgc cgtcgcgcgg atttctgttg gatacaaact
      961 actaatcact gcgccatatc caccatacat gccattgata aagtagccga ctatcacagc
     1021 ggctgctacc aaactccact ggctatgggc taacacaagt agatagacag ataaggatga
     1081 acaaattaaa aacaaagaaa aggctagttt aggccccaat ttatccatga ttatgccaaa
     1141 agtcatcatc cccaaagaca tgcctaaaat agtactgacc atccacaatg aagacccaga
     1201 aaccgaaagc cctaatcgat tttgcataat tgaaggtagc caattcatta acccaaaata
     1261 gccggcaatt tggacagtta ccatgatact aaggccacat gtttgccaag caagatgttt
     1321 tgattcaaac agtctgctaa gtttggtttt cttttcggca gattgtacag atttgaattc
     1381 ctttggttca gtcaagccgc gacgaatgaa caagaccatc aacaccggca gtacacctac
     1441 gacatacaac atcttccaac caaacgttgg aatgatcaca gcagctaaaa tcgcagccaa
     1501 agccgcaccg acctgcgcac caataccagc aaccgaagaa gctcgtccaa tttgtttttt
     1561 tgaaaaactt tcggaaatca aggacataca cgccccatat tctcctcccg ctcctacacc
     1621 tgcgataaag cgaaagagat aaaccaagta gatgtttgaa gcaaaataca tcaataaaga
     1681 agcaatcgaa aacagaagga ccgtttgaga aaatactttc acacgtccat atttatcagc
     1741 tatgatgcca aaaaaaatac ctccaatgag catccccaaa ttagtgatcg ttgaaatgaa
     1801 acccgcttgc gtaccactaa tatgaaaaga agtaatcatt gaagataatg aaaaagctaa
     1861 aaacataata tccatattct ctaaagcaat tcccgccgat gtcgatacta atacctttcg
     1921 ctgatacgtt gtcattttta tacccctttt cctaaaattt ccctaaattg ttaaaagtat
     1981 atccaaaaaa gaataaaaaa acaagacttt tttatttgaa ataatacaca aacaaccgat
     2041 atagataatc cagcttagtt ttaatagaaa tgagtattga cttggagtac acttaaagtt
     2101 gtagaataaa taaaatacat aagtaaagtg gcaagaaatt aagagaaaga gtgatcagat
     2161 gaatatcaaa caagtcagtg aagaaaaggg catttcggcc gatacattgc gctattatga
     2221 acgaatcggg ttgattccac cagttaatcg gacaaaaggc ggaatccgtg attatacaga
     2281 agaagaccta aaatgggtcg actttacgat ctgtatgcga aatgccggat tatcgatcga
     2341 gtctttgatt gaatatattc gcctatctag cgaaggagaa gcaactgtat ttgagcggcg
     2401 taagcttttg attgaagaaa gtgaactttt gacgaaaaaa attgctgaaa tgcaagaatg
     2461 tttggaacgt ttacaaggaa aaatcaagaa atatgatcgt gtgttatcta gaaataaaat
     2521 ggaatgtatc aagtaaaaaa atcaaggagc atcgctatgg agttacgctt actaaaatat
     2581 ttttggacaa ttgcagaaga aggaacaatc tctcaagccg cagaagttct acatcttaca
     2641 caaccaacct taagccgaca actgaaggag ttagaagagt aactaggaac agaactgttt
     2701 gtacgcgaaa accgcaaaat gatcttgaca gaagcaggtt attttctaaa aaagccgagc
     2761 agaagaaatt ttagatttaa cgaatcaaac cactcaagaa ttcgagaaca gaaaaaaaca
     2821 attattcagc ggccgattta cgatcggctg tgtggaagct gatagttcga atacattggc
     2881 actattacta gaaaagttga tccgtgatta tcccagcgtt caatttcata tccacagcgg
     2941 aacaagtgat gatatcacgg agtgactaga aaaaagcctg ttagatttag cgattttgct
     3001 agatccagta gatacagaga aatacgaaac gattttgtta ccaagaaagg aaaaatgggg
     3061 attgctcatt ttagaaaatt ctgttttagc aaaaaaagac gtgataacaa aagaagactt
     3121 tgcgaatgta cctcttttga tctcaaagcg tccagaaata cagcgaatgt ttgctgactg
     3181 gttaggcata aaaaaggagc agttgctgat cacaggaacg tacaatttga ttttcaatgt
     3241 atttgcgcta attgagaatc aggtaggtgc tgctttagtc atagaaggtg ctgtaagtca
     3301 tcattcaaag cgagtgacat tcgttccgct tgacccaatg attgaaacga actgcatgct
     3361 agtctggaaa aagaatcgga ttttatcacc tgtagtcaat gaattgattc accgtttcaa
     3421 agaagctttt gaacagtaat aagtatctct aatcgaagaa atttcaatac aaaagcgaga
     3481 cgtatgacag tcttgctttt gtattttttc atttttaaat caaaaaaata aagagattta
     3541 cataacttgt taaataagcg cttacaaaaa taagaagaac ggaaatgaat cagctataat
     3601 aaccgttgtt ctggattgta atcattacaa agaaatgaag attataactt tggaggtgtt
     3661 cgaaatgaaa gaaaaaaagc aaaaattcgt tcaaaaaaca tgccctttat tcaatggttg
     3721 ggtatgattc tgctgattgt attagcttta ttgacgatca ttcctttttt atggatggtt
     3781 tctgcttcat tcaaagcaaa taatgaagtg ttcacgattc cgattcaatg gatccctaaa
     3841 acttggcatt tagagaatta tctggttatt tggtctcgta ttcctttagt gacatttttt
     3901 aagaatacag cttttttaag cgttgtgatc acgttgattc agttgtttac ttctagtttt
     3961 gctgcttacg ggttttctaa actaaatttt aaagggcgcg atattttatt tttagcatat
     4021 atcggcacga ttgctgtccc ttggcagtct tatatgatcc cgcagttcat tatgatgaga
     4081 aaagtgggtt taacagatac gttgtggtcg ttggtgctat tacaagcgtt caatgctttt
     4141 ggtgtgttct tgttgaagca atattacagt agtatcccag attctttatg tgagagtgcg
     4201 cggatcgatg gcttatcaga gtggggaatc tatcgcaaga tcattttacc tcttacaaaa
     4261 ccggcgattg ccagtttgac gatcatcaca ttcgttaata catggaacga ttatatgggg
     4321 ccattcattt atttgtcttc tacggaaaat aaaacaatcc agttagggtt gaaaatgttt
     4381 attgggctat ttgatgcaga gtacgcatta atcatggctg cctcagttgt ttctgttctt
     4441 cctgttgctt ttatattttt agcgatgcaa aaatattttg tagaagggat cgcttcatca
     4501 ggaatgaagg aataggaggt gattatgaaa gagcagcgtt ttgacaaaaa tgtttatatg
     4561 gaattctttt attggttgta tcaatcattg gcaagtagcc tttgtttttg gtgtgtcaat
     4621 atcttgtata tagcagctgc gttccttttg tcgatagatg gaaggaatgt cttttccttt
     4681 attttttctt tactttttat tggtccaggt tgcatcgtga tgataggtat gacagaagaa
     4741 ttagcaaaag aaaatagttt ttcaccagta cgtttgttca taaagatgtt tcgccaattt
     4801 tggctaaagg gattttttta ttggctattt gcttggatag ttagtgtgat aatgattttt
     4861 gattgtttct tttttatacg tttttcttat ggaaaatggt tgattccgtt gtttgtttta
     4921 cttgcttgtt tatcagtttc ctttagcacc aactgctggt attttcaggt gagaaatcca
     4981 gcgtcgaaac caaatcaagt tctacggata gcgttctact atacgttgaa aaaatggtat
     5041 gtgagtttac tagatttttt actattgaca agtttgtttc tcttcttttt cgtaaagccc
     5101 caatggtgta ttctactagg tccttctatc gtatttggtt tgatttactt caacaatcgt
     5161 aaattgatga gaactatgga tctgtagact ttataaattg aaactttcaa ttaagaatga
     5221 cagttttttt tatgtaaaaa ctgtcattct ttttcatttt ttgaaacata tggctaaaca
     5281 aatgaaataa aacgtgctat acttttgtat gaaacaggtt tagaaactgt atcacttatt
     5341 tgtgattatt tctaactaat aattaaaaat aaagcataaa aaggacgatg ttcaatggct
     5401 gaaaaaatga aacaaggttc gaaatctgta gtagaaagtt tgattaatca tcatgttgat
     5461 tatgtattcg gtataccagg tgcaaaaatc gatggtgtat ttaatgaatt agaggatcaa
     5521 ggtcctgaat taatcgtgac tcgtcacgaa caaaatgctg catttatggc acaagcaatc
     5581 ggacgtatta caggagagcc aggagtagtt atcgcaacaa gtggtcctgg tgctagtaac
     5641 ttagctacag gtttggtaac agcgacagca gaaggtgacc cagttttagc aattgccgga
     5701 caagtaaaac gaagcgatct attgaagttg acccatcaaa gcatggataa tgcagcactg
     5761 ttccagccaa tcacaaaata cagtgcagaa atccaagatc cagaaacaat ttcagaagtg
     5821 attgccaatg cttatcgtat ggctaagagt tcaaaaaaag gcgcgagctt tatcagtatc
     5881 ccgcaagatg tagtggatgc acctgtccaa ggtaatgtca tcaagccatt gagcgatcct
     5941 aaattaggtt ctgcttctgc agatgatatc cgttacctag ctgaacgcat tagagaagca
     6001 aaactaccgg tactattagt aggtatgcga ggatcaagtg aaaaagaaac gttagccatc
     6061 aggcaattag ttgaaaagac agcattgcct gttgtagaga cattccaagc agctggagtg
     6121 atctcacgtg aattagaagc gcatttcttt ggtcgtgtgg gtttatttag aaaccaacca
     6181 ggagatatgc ttttgaaacg aagtgatcta gttattgcaa ttggctatga tccaatcgaa
     6241 tatgaagcaa gaaactggaa tgcggaaaaa gatgcgcgga tcattgtgat cgatgaagcg
     6301 ccagctgaaa tcgatccttt tatgcagcct gaaagagaat tgatcggtga tatctcagcc
     6361 actcttgatt tattgacagg ttcattagag cctcaacaag tgtcagaaga tgcaaaagaa
     6421 tatttagcat ctcttcaagc aaaattaacg gagcgggaca ttgttcaatc aaaaggagaa
     6481 gcaggtatct tgcatccttt agaagttatt aacacgcttc agtcaaaagt aacagatgat
     6541 atgactgtta ccgtagatgt aggtagtcat tatatttgga tggcacgcca ctttagaagt
     6601 tatgaacctc gtcatttgtt attcagtaat ggtatgcaaa cgcttggtgt tgcattgcca
     6661 tgggcgattt ctgctgcatt ggtgcgtcca aatacacaaa tcgtatcagt ttccggcgat
     6721 gggggatttt tattctctgc ccaggattta gaaacggcgg tacgtaaaaa attgaatatc
     6781 gttcatttga tttggaatga tggacactac aatatggtag agttccaaga aaaaatgaaa
     6841 tatcagcggg cgtcaggtgt agactttggt ccagttgatt ttgtgaaata tgcagaagct
     6901 tttggcgcta aaggaatccg agctacaagt gtagaggaat tagaaaaagc tttagaagaa
     6961 ggatttgcga cagaaggacc cgtaattatt gatattccga ttgattaccg tgataatgag
     7021 aaattgggag aaacaatctt accagatcaa ttctattaag gaagtgtcga catgactgaa
     7081 aaaatcttat accagcacgg gacattagga gctttgatgg ccggcttgat ggatggaaca
     7141 gaaacgattg ctcatatatt agaaaaagga actctgggat tgggaacatt gcatgggctt
     7201 gatggtgaag tcatcttttt agatggcgta gcttatcaag gaagatcaga tggctcagtt
     7261 gcccaattag atggaagtga acttaccccc tatgcagcaa ttacagattt tacgccagat
     7321 actagttttt ctgtctcaaa aactgcagat ggagaacaat tgaaaaaaga catattagta
     7381 agagaagccg gggaaaacct atttttggca gtgaagataa ctggattatt caaaaatatg
     7441 cacatccgta tcatgccgaa gcaagaaaaa ccttacagaa gactagcaaa aatttcagag
     7501 tctcaaccag aattccaaca aagcaatatc caaggaacat tagtcgggtt ctttacgcct
     7561 gaattattcc aaggagttgc tgcagcggga tttcacctgc attttatcga tgatacacat
     7621 acatttggcg gacatgtaat ggattttgaa gtggctgaag gaaaagttga gattagccgc
     7681 atctcgtccc ttgtacagca ttttccagtt gatgatccga cttttgttca cgaaacgatt
     7741 gattatgctg atttagcagc agaaatccaa caagcagaat agaaaaagac agcgagatgc
     7801 gttcaaggtg attaacctga cgtatctcgt ttttttgagg aggaaaccta gcgtttacaa
     7861 aaaaactgag ctataatctt ctccatagat caaaaaggag cggtttttat catgtggaca
     7921 gaaaaacaga aaaaagatgc atacgaaatt ttattattac agcaacaagg attattagaa
     7981 gcagaagata actggttagc aaatttagca aattcatctg cattgttgaa tgagactttg
     8041 cctgagactg tctttgcagg atattattta ttcgatggta atgaattgat tttaggaccg
     8101 ttccaagggc gagtttcttg cacacgtatc aagatgggaa aaggagtttg tggagaatca
     8161 gctgaaaaac aagaaacatt gatcgttgat gatgtcaaaa cccatgcaaa ttacatttcc
     8221 tgcgattcag ccgcacaatc agaaatcgta gtgccaatgg taaaagaagg taatttaatc
     8281 ggtgttttag atatcgattg cggagtaacg aaagggtatg atgaaatcga tcaagcattt
     8341 ctagaaaaat atgtagatct tttattagca aattttaata taaatgcttt ttcatttatt
     8401 aataactaaa atcgtctatt gaaacaaaaa agaagagaaa tcaagaataa aaagagccgg
     8461 aaagacagtg gtcatttctg tttttcggtt tttttatttt tttataaaat cacaagtgtt
     8521 attgattttc acaatcatat tttaatggta ttatcatttt gaataatgaa aaaacattaa
     8581 ggatggatga aatatgaata acgaaactat cattttagga aaaaaatata tttgccaacc
     8641 tattggcttg aaacaaaaag tggttggaga agtaatcagt aaattagaaa attgtgttgt
     8701 gatctgcgta gacgaatatc atgacgtcga tcgcaatgaa atattagaaa aatgcggaaa
     8761 agtagtggca aaatatactc acgtttatga gttggaaaca acagaatatc tatataacta
     8821 tagcaacaaa tatgaacata ctttagtttc ataaagattt aaataaagtg ccttcaaaag
     8881 cgagaagacc aagcttttga aggcacttta tttagttggc agtgattgtt catgattttt
     8941 gatttattca aaaggttcgc ctagagaatc tgtctctgta acgaggcctg gtggagaaac
     9001 tcgataaaaa cctatactgc cagaactgtc ctattttcgg atagacaaac tagtagtttt
     9061 aaaaagatca tccgatccaa tcatttcaaa aaatgcaaaa ctgatatcag gatcatttag
     9121 ctcaggcttg ttatcgtaaa gcatttgtaa aggttgcatt tagttaggag taaacgcgaa
     9181 actatcatca ttatttgctg aagaagatga agaagctaat tcagagtttg tttgaaataa
     9241 aggagacgtg ctgacagttt ctttaccctg ttttttgcag aggtagaaac ttttatatcg
     9301 gaagtttcta ccggagaatt tttctcattt gtagaaattg ttgaatcgat actattcgaa
     9361 atttgcgtat ctgaaggtat gttctttgat gcagaagtcc cacaagcagt aagtaacaat
     9421 aaatttgata aactgataaa cagaattctt ttcattatgt acacactcct tatagtatcg
     9481 tttagcatac acctatgctc agtattaggc aaaatcaatg caaaactatc aagtctttta
     9541 tagtatctat tagtcttagt aatacatata tggagagggt gatatcccag ttcaaaccag
     9601 atacattggt taccatctgt attaacgatt atgcctctaa acgctttttt tatgaaaaaa
     9661 tttataagtg aaaacgtagg tatgatatta tctagaggta atttttttgg gtatttaaat
     9721 agttgctagg aggatagctt ttggattttt acagtaattt tatcttaata atagcaatat
     9781 tattacttct aaatatttgg ttttttgata agagtagaaa tgcagggata ggttttagaa
     9841 ccaaacgaag tacgtcttct gaaaaaaagt gggtatattc acagactatt ttttacggag
     9901 gggttatttc aatcagttta ctttcttcaa cactttattc ttttaatgtt atagacgttt
     9961 ccatgtcaaa ttttatatct attattggaa tccttatttc tgcaattatt acacaactac
    10021 ttcttgtgtt tgaagagaag tcaaaaaata attaaagcaa ttatgccttt tagtacagga
    10081 attattactt cttacaaatg tatagcgaat gtgaagcctg ctgttttaat tgttctagaa
    10141 aaacttttga gtgaacaatt attttatgtt aggatgaatt aatttctcta gagtttataa
    10201 ttaaaaaact tatttgaagg aacatgctta aaccataatt gtgtttttcc ttccatcagc
    10261 ataaaagttc acctcaaaaa gagtgtacga acaaaagttc tttttgtaaa ccttaaaata
    10321 gattttcctg aatacgtatt ggggatttct aaatgtttga atgttttttt gaatgcaaaa
    10381 tcaatgcaaa aaattttgta tttctctact tttctctcct tgaccaaaat aaaaaaacgt
    10441 tgatttaaca acgtttttca tacatgttta tctgtctcaa ccatacatat atggagacgg
    10501 cgggagtcga acccgcgtcc aaacacatcg ccacttaaaa atctacgttc atagtcaact
    10561 catttaagga ttcactttac agcttgccga gtgacaggca ttctgtatcg ctagtctgat
    10621 gttctctttt tcaacttaca gacggaaagt tgaaacgtat cccactaaag ttaggaccct
    10681 tactcgagca catgggcgat gccgagagga tctacgctaa ctgtttttag gcagctaaag
    10741 cgtaagagtt gttttcgttt ttagcagtta tatttaactg taacgttttt acgtagacgt
    10801 aacctacgaa acgcaattca agctcgactg tgcctgtcga atccgtaacg tccccgttag
    10861 ctaacaaggg ttacgtgtat ttttcataca aaattagtat agcatatatt gggataaatt
    10921 caataaaaaa gcgaatcata tcccattatt tatataataa ctgccccttt cttaagaaaa
    10981 aataagcgat tttcttaaga aaagaggtag cttcaaaaaa agatcttaaa tcagatcaaa
    11041 atacttcaat gcgtggacaa ttccgccttc tgtatttttc tttgtgataa aggtagaaag
    11101 ttcttttaat ccgtcatagg cgttgcccat agcgattttg ttgtcgcaag cttccaacaa
    11161 ggcaaaatca ttcggaccat cgccaaatcc aaaagtagga actttttcca gctgaagttc
    11221 tttttgcaga cgtttgacac ctgccccttt tgagattcct ttcttcactg tatcgatcga
    11281 gtaaggacca ttccgataaa aagttagctc aggaaaatgt tcgtgatagt attcgtcacc
    11341 tgattgagat aagatcagca acatatttac tgtgttttct ttaaagccta aaggatcaat
    11401 ttccggaaca tctgaatgaa tgaagtcgta agcattgatc attgtttggc tatgaccggt
    11461 acaccagatt ttctgatcgt tatagtaaga aagttcatga ccgttcaact gtacatgatc
    11521 atacattttt tgacattctt ctgaagaaaa tgtttgagaa tagatttctt cgccgtctac
    11581 tcgaatgaaa gaaccattca tggtgaccgc agaagtaatg cccgcatctt ccatgataga
    11641 gtggatttct gcttctgtac gtcctgtagc gatgacgggg agaacattgt tttcttttaa
    11701 tgcgttcata gcttttgcca cttcgggtgt gatttttgat ttcgcatcta gtaatgttcc
    11761 atcaagatca aagaaagtga ttgcacgata atttgacaca aataaaactc cttttttatt
    11821 ggaataagtt tatcttaaca aagaatgaac aaaagacaaa cctttcgtga agaattcctg
    11881 aacatattgg ttcagtcaac aatttagagt atactataag aagagaattt aggagggaaa
    11941 gaatgatttt tttcagtatt ggtatcttat tcttaataat tggcagtctt tttcggattc
    12001 tgccctcaaa aggaaatctg cctttttatg ggtatcattc gcctcttgcc gcaaaaacag
    12061 acgctcattg gcggctggct caaaagacaa gcggaaactg gttttttctt atgggcttac
    12121 tgatggcatt gatcggctat tatttaaaga catctgggca tacgaattat tttctgattg
    12181 aaatgcttct gctagtattt ccaattatgc ctatttttat tatgacagaa aagaaattac
    12241 agaaatatga cctggaaact ggagggaacg ataatgaata ttttaatgat tgaagataat
    12301 caatctgttt ctgaaatgat gcagatgttt ttcttaaatg aaggttggga agcaactttc
    12361 aagtatgatg gaaaagaagg gctcgatgcc tttttagaaa acccacaaca ttgggatatg
    12421 attacactag acctgaattt gcccacactg gacggtatgc tagtaggaag agagatccgc
    12481 aaagtttcaa gtactgtgcc gattatcatg ctgacagcta gagattctga aagcgatcag
    12541 gtgatcggct tagagatggg ggcagatgat tatgtcacaa aacctttcag tcctctgacg
    12601 cttattgctc gaatgaaagc tttgcatcgc cgtagtgagt tggcagaagc aagagaagaa
    12661 gcagcagttt cagatgatca ttttgatatc gagactagtc atttcaagat gaatacgaaa
    12721 acacgagaag cgtatctgga caaccggcca attgaaggac tgacgcctaa agagttcgat
    12781 ctattgttta ctttagcaaa aaaaccacgt caagtattct ctcgggagca gttgcttgaa
    12841 ttagtctggg attatcagta ctttggtgat gaacggacag tggacgccca tatcaagaaa
    12901 ttacgacaaa aaatcgaaaa ggtcggacca caagtcatcc aaactgtttg gggagtcggc
    12961 tacaaattcg atgattctgg tgttgcctaa tgcgttattt gtatcagcaa ctcttagctt
    13021 tttgcggaat ggtcgcgttg atcttattga tcgttggagt ttcttttaca caactgacaa
    13081 aacaaacgat tgaagaaaac aattaccagc aactgtttgg atatgctgaa tcagttgaaa
    13141 agacaacgca aacctatgcc gatacgctcc cgcagtttag tcaagatcag gcgtttcaaa
    13201 atgcattgct gctgacagag caagtactga cagaacaaaa tgtgaacttt atatttatcg
    13261 ataaaaacga acgtgtcatt tatccgacta atacagcttt actgaatttt accttaacag
    13321 ctgatcaatg gaaaaatctg aggaatggac gtcaagaaaa attcacatcg aataaaaata
    13381 ttttaggaca agcacaggcg acctcttacg cacttgtgcc atttaatttg aatcatgaat
    13441 tctatggagc acttgtagtt agtcaacctg cgcgaaatat cgataacagc gttcgtccaa
    13501 ttacactgaa cctgttcaaa ggatttattt tttccagtat cgttgcagtt attgccagct
    13561 atgcttttgc agcattccaa gttaagcgta tcaatcggtt aaggaatgcg acgaaagaag
    13621 tgacgaatgg taattttgat gtacagctcc ccgtacatga caaagatgaa tttgatgagt
    13681 tagcagatga tttcaataaa atgacgaatt cattgaaaga atctcaagca gaaattgaag
    13741 aacaagaaaa tcgtcgaaga caatttatgg cagatgcttc tcacgagatg agaacacctc
    13801 ttacgacaat caacggactt ctggaagggt tagaatacaa tgcaatccct gaaaatcagc
    13861 gagaaaacgc gattaaattg atgaaaaatg aaacagagcg tctgatccgt ttggtgaatg
    13921 aaaacttgga ttatgaaaaa atccgtacga atcaaatcag tatggtcatt aaaaaattca
    13981 atggaacaga aacattgaaa aatattgttg cacagttaga agctaaagca gaagcagcag
    14041 gagatacatt gacattgaaa gccgttgatg atattgatgt ttacgctgat tatgaccgat
    14101 ttgtccaaat catggtcaat attattcaaa acgcgataca gtttactgaa aacggccaaa
    14161 tcacgattac tttagaaaaa ggctatcttg aaacgatcat cacgattgaa gatacgggaa
    14221 ttggaatgtc tgaacaacaa atgaaaagta tctgggatcg ttattataaa gtagatcctt
    14281 caagaaaaaa tacaaaatat ggtgaatcag gattaggttt accaatcgtg cagcaacttg
    14341 tacgcttgca taaaggaaaa ttagaagtag aaagcgaact aggaaaagga acaaaattcg
    14401 ctgttcgttt gcctgacgta gaaatcaaaa aagagtaaaa aacaagcggt ataaaagatc
    14461 atgaatcgat cttctatacc gcttgttttt actataaaat gattgattta gcccgcgggc
    14521 tctaaacgat caatataagg actttgagca atagtagcat catgagtaaa gagtaagata
    14581 ccttgatttt cttcatgagc taaataagaa agcaagtgga gtagctcttg gatctccaat
    14641 acactaagtt catcaaatgt atcttcaata acaatattgt ttcgcttgga aagcaaggca
    14701 tgaagtaatt gcagctttac tttttcaaaa gacgtcaact cagctgccgc acaattaagc
    14761 aaggaaggag agatatcaag tttttcttga taggccaaac gatcttgttt gatttctttt
    14821 tccggtacat ccaacaggac atggtcttta agattcagat aaggaaacat cggccaattt
    14881 ttttggatga tcgcaaaatt atccagtttt gcaacaagaa cctctttttt caagtgctcc
    14941 aatattttat gctgtttttc gagaggacac aaaataacgg tcaagtgttc agcatgaatc
    15001 aaatattcag gtttcattac gtccaggcct cctactttgc aaatgatgga taaaaaaagt
    15061 gaaagggaat agactaagaa tgagacagca tccactgagc ccaataagag tttgggcaaa
    15121 tcccttaagt atatctgtga aaaaaagatc attttggaaa tcgatggtaa aaaaagtctg
    15181 ttgatccatc ggaattgtaa gtccgcttac cgaagtcaag atatcacgga tatctggcag
    15241 ttcagtagtc tttaagacaa gaaaattgat cctttgaaga agcgcttcat aaactgactg
    15301 gaagcttacg atccacaata aaaccaagat acaacaaaga aagagaggaa agaacagctg
    15361 ccagaaaata aaaaggaata atttggtttt agaaagacca gtcaggcgcc aattgatcaa
    15421 ttcctgcttg ttttgcctga ctgaatagta aaagaaaaag ataaaaagga aggcaaatag
    15481 aaaaaataaa ctgaatatgc cttttatata aaatcggcta gctaattcta aagccggaag
    15541 aagctctgta gatgcagaag cgccaataaa tgaaagccga tcttggactt gattggctaa
    15601 agccgattgc aaatggtaaa gagtcaggca tgctatcaat aaaaaagcag aaaagcttga
    15661 aaataaacaa tagagtattg tctgcttttt gtaataaatt aatcgagcag tagcatagcg
    15721 gattattttc atacttttca cctctataaa aagtatagaa aatgaatatg aataaagtat
    15781 gaaattcctt tagtgaaatc acgaattaaa accagaaaat attttgtaaa aagcaagagg
    15841 ttgtgaagaa gctgccctat tctattaccg tagatgcaag cttttgaaca ctgtttattc
    15901 gaaaagagga gcagcgacaa taattttgtc gctgctcctt ttaaatttat tttgtcattc
    15961 ggaagaactt gtggtcgttc cgtgaagttc ttggtacgtt tgcgttttat aaagatcaac
    16021 agttgattgt ccgttgtgtt cactaaagac actagttgac ttgtcaccaa gagatttttc
    16081 agcttctttt aatttctcta agccattctt gtagtcataa tcactggtat caattgggtc
    16141 taatccacta ttcgtataga accgaagaag atcaccatta ttgatttggt cagaaacagc
    16201 taactgttta tcaacttttt ctttcagtga atcaatttca ttctgtactt cctcagtagg
    16261 ttcagtaata gcttcaccag tttcgttact atagatcgta ccaccgtagt aagtatattt
    16321 tggtgtaaca aaatcaccat cacggaaaga aacaagctgt tcatgatctt tcgagaatag
    16381 atcttgtcct aactgaatat aattttttgt atctacacct agtaagtgaa gtaatgtagg
    16441 caaggcatca acttgtccgc cgtaagtgtg attgattccg ccctgtgttt gtccagggat
    16501 atggatcata taagggacac gctgcatttg tgcattgtcg tagtttgtcc aagtagaact
    16561 tgtttttcca actaattcag ctagattttt atttcttgaa ttcgagacac cgtaatggtc
    16621 accgtaaagg acgatgactg aattatcata caagccgctt tctttcaaat aattgaagaa
    16681 ttcttgtacg gcggtatcta aataattggc tgtcgcaaag taaccgttga tcgtttcatc
    16741 atttgtattg gctaacggga atcctgcttc gtcattggtg aattttgaat aaggataatg
    16801 gttagataca gcaatgaatt ttgaataaaa tggttgttgc agacgttcca agtattgtac
    16861 ggattgttgg aaaaatggtt tatcatgcaa cccgtattgg aacgaattgt catcattaac
    16921 atcataataa gaagaatcaa agaaataatc atatcctaaa ttcttgtatg tctcgtttcg
    16981 attccagaat gtaccagcgt tcccatggaa taccgcacta gtgtaacctt gtgtttgact
    17041 taaaatatca ggtgcagctt ggaaagtatt ttttccgcct aattgtgtga acaaggagcc
    17101 ttgattcaag ccaaacaatg aattttccat cagcgtttcc gcatcgcttg tttttcctgc
    17161 ttttacttga tggaaaaagt tgtcaaaact gaacgtgctg ttgctgtggt aaagactatt
    17221 gatgaaaggt gtaacttcat gctggagacc attctcatct gtcaatttat aatcgatcag
    17281 gaattgctgg aagctttcta aatggatata aatcacattt tttcctttag cgagcccgaa
    17341 caaatcgtca tttggtgctg cataatgttc ttttacataa ttttctactt ctttcatatc
    17401 attgggactt gcttctgccc gcacttggtt tgtctggtaa gtttgaacac cgtcataaac
    17461 catgaaagca ttcaagccaa gatatttgac caaataatca cgagagaatg ttcgagtcag
    17521 caattctgga cgatcagctt ccgccataaa aagatttcct gagaaaatca taactgctaa
    17581 tgtagagatc gcaaaagcca ttcttgctcg gatcgggcga gggtccaact tgattttttt
    17641 tgcaatcagc agaacagtca agataataaa atcgatccaa tagatcacat catatgggcg
    17701 aaacagtcga atggcacttt ctcccagacc actcgctact ttcccagcac ctaaaatcgt
    17761 gttgatcgta atgaagtccg tgaattctcg ataataagtg acattcgaaa aaacaagcaa
    17821 tgtcaaaaga aaataaatga ccatcatcgt gatataagca actctagggc gtctgatata
    17881 aagagcaatt ccaagtaata acaatgtagt tgcaataggg ttgataaaca agataaagta
    17941 ttgaatgcca ctttcgattc cgagatggaa atcaactaaa taggcaaaaa tcgttttcaa
    18001 ccaaagtaaa acgactagta aagaaaaaaa tccgagcctc ttattcaaaa aggcaggtgt
    18061 ttttatacat ttcaagataa tcttcctttc tgcacttctt aatcacagaa gtgtcatgaa
    18121 accatgttac acagctcact tattctcatt tattttacta gtatgacaat atagtgtcaa
    18181 ttgaaaccag aagtttatgc tgtttcatta aggttttctt taagcttcat gaaaaaatga
    18241 gcagtaagac aagtcctatg ttttttgtta tacttgggaa gagaaagcta aagcaggtga
    18301 aggaatgaaa gtaaaagtaa aaaaaagagc aagcaatcga ttgaaaaaag gatatccact
    18361 gatccaaaaa gaagaccttg ctcatcccca aaaagaaacc acgcaatggg tggaattagt
    18421 tgattcgcaa aaccaattta tcgcaaaagg atatctagga aagcagaata agggaatcgg
    18481 ttgggtattg agccagcaga acatcccatt tgatcaatct ttttttgaag aattttttgt
    18541 gaagtcaaaa gaaaaaagaa ctttcttttt ttcagatgat tctactaccg cttttcgtat
    18601 attcaatgga gaaggagacg gaatcggcgg tctgattgtt gattattatg caggttatgc
    18661 tgttttttct tggtataacg aaacgttata tgcacatcgt gaattattga ttcaagcttt
    18721 tcgtgaaatc tacccggaaa tcgtaggagg ctatgagaaa attcgatttg aatcagtaaa
    18781 tctgcctgag agccagttca tttacggaaa agaggcaccg gaacccttga tcgtaaaaga
    18841 aaacggagta aaatttgccg tttatttaaa tgaaggatta atgactggga tctttttaga
    18901 ccaaaaagaa gtacggggat cattagcaga aggtttcgct tctgggaaaa cagttttgaa
    18961 catgtttagt tataccggcg ccttttctgt tgctgccgca atgggagggg caatcgaaac
    19021 aacaagtgtc gatctagcaa aacgcagttt aaaaaaaaca caggaacagt ttgaagtgaa
    19081 tggtttggat gtcgatcagc aaaaaatcat tgttatggac gtattcgagt atttcaaata
    19141 cgccaaaaga aaacaatttt tatatgacat gattgtattg gatccaccaa gttttgccag
    19201 aaataagaaa aaagtctttc gcgtagcaaa agattatggg gaactggtca aggatagtct
    19261 ggatatttta gcagaagacg ggatacttat tgcgtctaca aatgctgcga atgtctcgat
    19321 agaacaattc caaacaatga ttgaagagga attccatgcc aaaaatgttt cttttgaaca
    19381 acttgcttta tatcgtctgc caaaagattt ccagacgacg gatacctttt tagaaggcaa
    19441 ttatctgaaa gtgtttgttt atcaaattaa aaataaccat tagaaggagc gatgcagaat
    19501 ggtttgggta aagggattga atataggaag cggtagacca aagatttgtg cgcctcttat
    19561 cggggccgtc cgagaggaag tattacgtga agcagttctt gcacgtgaag caggggccga
    19621 tttagtcgaa tggcgagtcg atcattatcg tgaagttttt tcttttgatg aagtagcaga
    19681 aacattggtt cttatccatg agatgcttga tggactgccg ttattattta cgtttcgtac
    19741 cttagctgaa ggcggagaac aagacatatc gatccgtcag tatcgcgacc tttatgaaca
    19801 tgtgatacac acaggattag tagatatggt cgatatcgag ttgttcaaag tagaaagtct
    19861 aggaaaaaga ctgatagagg aaatcaagtc tttaaacatt cctttaatta tcagtagtca
    19921 tgattttaaa gaaacaccag cagaccctgt cttgctttac cgattgaatg ttatggaaca
    19981 ttttggagcc gatatcggaa aactggcagt aactcccaat aatgaacgag atgttttgcg
    20041 gttgatggag ttgactagac gggcaaatgc ttttgtctcc atgcctatta ttacgatgtc
    20101 catgggaaat cttggtaaaa tttctcgtct ggctggcgga acgacgggtt ccgttatgac
    20161 atttggttcc ttaagcagcg attctacttc tgctccagga caattaccgg tcaaagcatt
    20221 gaagcaaatt attcaattat taaatgaacc gttgtagaac aagtttgagg tgatattttt
    20281 tggcaaagaa aaaaatagta aaaacgaatg caatccgtat ggtcgaacaa aaaaagattc
    20341 cctatacaga gcacgaatat gagtgggacg aaagccacct aagtgcgtca tcggtcgctg
    20401 aacagcttcc agaaagccaa tctcgtatat ttaaaacact ggttgctgtg ggcaatgtga
    20461 cggggcctct tgttgctgtg ataccgggag aagccgaatt gaatctaaaa aaattagcaa
    20521 aagtttcagg gaataaaaaa gtagaaatgc tccatttaaa agatttggaa gcaacgaccg
    20581 gatacatacg tggtggctgc tcgcctatag gtatgaaaaa actatttcct acttatttag
    20641 atcaaattgc agaaagctat gaacaaatca ttgtctcggc tggcagaaga gggctacaga
    20701 tggagttagc acctcaagat atctgtgcat taacgagcgg acaatttgca gatataaaac
    20761 aataaatcat acgtataaaa gaaagaagga ggccgggatg ttgtttgtcc agtctccttc
    20821 tttctttttt atacattatg ctctttgccc ggcattcatt cctgctacat gccctgtagc
    20881 gaaagccgct gtgatattaa agccgccagt ataaccattg acatccagta attctcccgc
    20941 aaaatacaga ccatcaatga ttttactctc caatgtttta ggataaattt cctttagaga
    21001 aacccctccg ccagttacaa atgatttctc aataggaaaa gttttgttga ttgatagctt
    21061 aaactctttg catagttgaa caaaatcttg aatttgtttt tgagaggctt gttgtcctgt
    21121 cagatgatcc atttcaagcc gctcaagaaa gaaaactaac aatcgctcag gcaatatacc
    21181 atgccaagca ttgaccaagt tctttttggt tgctttactt ttttctgtca atatatgaat
    21241 aagctcttgt ttggtttgtg tagggaaaca atccagagaa agtgtcactg gttctcccgt
    21301 tttctctaat tctttattga caaaacttga acaacgcagt gcagccggac cagaaatacc
    21361 gaaatgggtg aaaagtaaat ccatcgtatg ttcagtcaat acacgacctt ttttatttaa
    21421 aacgcgtaaa gtgatgtctt gtaaagaaag cccttgaagt gttttctcct gaatatacgg
    21481 ttcatccgat atcaaggggg actctgtcgc ataaagaggc gtcaccgtgt gacccactcg
    21541 cttcacgatc ttgtacccat ctccagtaga accagtagat ggataagtcc gtccaccagt
    21601 tgttaagata atggcaggag catggaactc ttcgaattcc gtgcgtatac cgtaaatttt
    21661 atgttctttg tggatcaatt tctctactcg agtagagaat agaagcgtca catctagttc
    21721 tttcagacga ttgagcaatg cttcgataat cgtttttgat ttatttgtta caggaaacat
    21781 ccgtccatga tcctcttctt ttaaatgtac gccttgtgat tcaaagaagt tcatgatatc
    21841 gaaattattc cattgcgcaa acgtactata taaaaacttt ccatttccag gaatatgtgc
    21901 aatcaaatca tcaacaggac gattgttcgt aacattgcac cgaccaccac ctgtcatcag
    21961 tagttttttt cctgcttttt tgtttttttc gatcaagagt acgcgtgcgc cttgttcagc
    22021 tgcactgatc gctgccatca tcccgcttgt tccagcgccg actacgatca catcatacag
    22081 tttttccatt ccctcacctc aaggaaagtg tagcatatct gaagaaagaa aacagaaaaa
    22141 aaatgttttg agccaaaaga gaaaatgagc taaaatgagg gaagataagc aaaggagcgt
    22201 ggaacgatga caaacgcaac agaatatgtt caagcaattc aagaaaagct acatcaaaag
    22261 gatcaagggc aagaagaatt tttacaagca atcgatgaat tcatgcccac ggttatgtct
    22321 tttttggaca ctcatcccga atatatcgaa aagaatatct tagaactttt aacggaacca
    22381 gaaagagtta ttcaatttcg agtgccttgg caagatgatg aaggaaactg gcgagtaaat
    22441 agagggtacc gtatccaata caactcagct atcggccctt ataaaggtgg attgcgcttc
    22501 catcctagtg tcaatctaag tatcttgaaa tttttggctt ttgaacagat tttcaagaat
    22561 agtttaaccg gtctgccaat cggtggagga aaaggcggaa gtgacttcga tcctaaagga
    22621 aaatctgata atgagatcat gcgtttttgt caaagtttca tgctggaatt gtcaaaacat
    22681 atcggcccat cgattgatgt tcctgcggga gatatcggtg taggagctag agaaatcggc
    22741 tatttgttcg gcgaatataa acgattgaaa aaatatgata ctggtgtgtt gacaggtaaa
    22801 cctttggatt tttggggaag taagatacgt acggaagcta ctggttatgg attagtgtat
    22861 tatgtcaaac atctattgaa cgaagaaaaa gattcattta atcaaaaaac agtttttgtt
    22921 tccggaagcg ggaatgtagc catttatgca atcgaaaaag tgcaagagct aggcgggaaa
    22981 gttgttacat gttcagattc tagcgggtac atctatgatc cagaaggaat agatgtgaaa
    23041 ctattgaaag aagtcaaaga aatcaataga caacgtttga cggaatacgc aaaccaacgt
    23101 ccttctgcta tttatcatgc tggagaatct gtttggacat tgaaagaaaa agcggatatc
    23161 gctttgccat gtgctactca aaatgaaata gacgaaagct tggccgaaat cttagtagaa
    23221 aatgggatca aaatcgtagc agaaggcgct aatatgccat gtacagtaaa agctgtttct
    23281 gttcttcatg aagcaggcgt gtggtattgt ccaggaaaag ctgctaatgc tggaggagta
    23341 gcagtatctg ctttagaaat gagtcagaac gtccagcgat tgacttggga aagagagcaa
    23401 gtggatgagc agctggatca aatcatggaa acgatttacc atacgtgtcg ccaaacagca
    23461 gccgagtacg gcgacgaaaa aaatctgctt cttggagcaa atgttgctgg atttgaaaaa
    23521 gttgccaaag caatggcagc agaaggttta gtttgaaaaa tgaaactttc ttggaaatat
    23581 tgtcagcatt ttgtttattt gcttgaaaaa taataaaaaa aaacgtaaag tattacctgt
    23641 agagtaaacc tataaggagg agaacaaatg gcacacattc attttgacta ttcaaaagta
    23701 gcaccgtttg tcaacgaaca agagttaggg tacatgcaaa gccaagtaac agcagcccac
    23761 aacgaattac gggaaggtac tggcgcagga agcgacttcc gcggctggat cgatttacca
    23821 acgaactatg acaaagaaga atttgcacgt atcaaagaag cagctaaaaa aatccaatct
    23881 gattctgaag tattagttgt tatcggtatc ggtggttctt atttaggcgc tcgtgcagcg
    23941 atcgacttct tgaatcacac attcttcaat ttattagata gcaacacaag aaaagcacca
    24001 caaatcttct ttgccggaaa ttcaatcagc tcaacatata ttgctgactt gatcgaagta
    24061 atcggtgatc gcgacttttc agtaaacgtt atttcaaaat ctggaacaac tactgaacca
    24121 gcgattgcgt tccgtgtatt caaagagctt cttgttaaga aatatggaca agaagaagca
    24181 aacaaacgta tctacgctac aacagataaa gcaaaaggtg cagttaaagt agaagcagat
    24241 gcagaaggtt gggaaacttt cgtgattcct gatgacatcg gcggacgttt ctctgtatta
    24301 actgctgttg gtttgttacc aatcgctgtt agtggtgcag atatcgatgc attgatgcaa
    24361 ggtgccgctg atgcaagcaa agcatactca agcgacaaac tagaagaaaa cgaagcttac
    24421 caatacgcag caatgcgcaa cattctttac cgtaaaggca aagtaactga gttgttgatc
    24481 aactatgaac caggaatgca atacttctca gaatggtgga aacaattgta tggtgaatct
    24541 gaaggaaaag accaaaaagg catctatcct tcaagcgcaa acttctcaac tgatttgcac
    24601 tctttaggac aattcatcca agaaggacgt cgcaatattt ttgaaacagt tgtgaaggtt
    24661 gaaaaacctc gtaaatcaat cacaattcct gaacaagcag aagatttaga cggtctaggc
    24721 tacttgcaag gaaaagaagt ggacttcgta aatacaaaag catttgaagg aacattgctt
    24781 gctcatacag acggtgacgt gcctaacttg ctagtcaaaa ttccagaaat ggatgcttat
    24841 acattgggtt atacaatgta cttctttgaa attgctgtag gtatttcagg ttacttgaat
    24901 ggaatcaacc cattcgacca accaggtgta gaagcttaca agaaaaacat gttcgcactt
    24961 ctaggcaaac caggctttga agatcttgct aaagaattga acgaacgcct gtaagataag
    25021 ggtttttcgc tagatttaaa taatcagtga tagaaacatg ttcgctttaa aatgaaaact
    25081 ggtacccatt taggtactca aacatactgc atcgtttatt cagaataagg ggctgtgaca
    25141 agacgtttgt cacagtccct tgtttgtata gaaatcgatt gattgccttg ctctttagtt
    25201 tgttttagga ctttaagaac agtgaggctg acttcgaagg ttaattctgt ttttttgttt
    25261 tctagaatca gtaagcaaaa aagcactcac ttcgagtgct tttttgcgat atattcttag
    25321 cttaagatag gcctaatttt tcttttacag atgagggtac cttatcgctg gtaattactt
    25381 ttttctctat gatccgcttt tcgcttgctt tcaactgcaa gatcgttcct tcggggtaag
    25441 cagaaccatt ggcagagtca ccagaagtaa tgacatcgaa atctaactgt cttttttcac
    25501 cgttttcagc ataagcatct acgttgtatt taaaacctct tccaattact tccccattat
    25561 ctgctttgat atcagtttct gcaggcattg gagctttgac taccgcataa taatctttac
    25621 ctacataagt agactgatag tagtcccaac ctttccatcc tataaaagca atcaatatga
    25681 taacgatcaa ccctaaaatt tttttcatga aagatctcct tttttatttt tattataccc
    25741 gtggtgctag ttaattttaa tatcctaaag aatatctcaa agttccttca aaacgtcgtt
    25801 gtgctcgact taatagaaca ctgtaagcca gtaatatgct ttcaaatcta ctctctagcc
    25861 ctttaacaga acgtgagttg aaattttgac aacctaattt ttctaaggaa gaaaaaacag
    25921 tctcaactg
//
LOCUS       DARBET010000033        25808 bp    DNA     linear   BCT 07-NOV-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4027
            SAMEA114569841-rid20112183.denovo.033, whole genome shotgun
            sequence.
ACCESSION   DARBET010000033 DARBET010000000
VERSION     DARBET010000033.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA114569841
            Sequence Read Archive: ERR12203666
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 25808)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 25808)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001972618.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001972618.1
            Genome Coverage       :: 132x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/07/2023 13:18:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,041
            CDSs (total)                      :: 2,975
            Genes (coding)                    :: 2,908
            CDSs (with protein)               :: 2,908
            Genes (RNA)                       :: 66
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 67
            CDSs (without protein)            :: 67
            Pseudo Genes (ambiguous residues) :: 0 of 67
            Pseudo Genes (frameshifted)       :: 26 of 67
            Pseudo Genes (incomplete)         :: 28 of 67
            Pseudo Genes (internal stop)      :: 20 of 67
            Pseudo Genes (multiple problems)  :: 6 of 67
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..25808
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA114569841-rid20112183.denovo.033"
                     /isolate="VRE4027"
                     /db_xref="taxon:1352"
                     /country="Denmark"
     gene            168..2228
                     /locus_tag="SCJ86_001537"
     CDS             168..2228
                     /locus_tag="SCJ86_001537"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300532.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bacterial Ig-like domain-containing protein"
                     /protein_id="HEG1169078.1"
                     /translation="MCSKRRSKQLNKHACRLDKERSCSEKHYVKFMMRTMLISLSVGV
                     YMHVPVQATTQTIKVVRTSVSETTDSPITITHTFTDANGKQLLPALTSYNKVSYPPTI
                     NHYWLIPNKTLWTSETGSSVPLSIFSDMTDPTKGDQLKQITDILNHALTDIKDGGSAQ
                     VSYVYEKDYSQFERKKIDVLAGTKVSISDLVERLKNADGTQGDMSKVISNNGNIVDTT
                     NTGTSTISLSYFDPIALKTMTASAVIHVRADQTAIKGKDLHLRTGESYAPKDLVEYVT
                     KSDGTSGNKEEIKMNGTVDTTTPGEYPVVLTYIDSKTSNVAETTAVVYVEASAASSTV
                     ITHFIDSQTNKELQAETHMSSVNRIPDLRLPAEFSDRVPNIDLSTYEQNGKVTSISDW
                     MQANGLDRKSHWSEILEVLHSELMNNRKQSGGEITFTYVYIPDNRSLEGHDLIVHPNE
                     KINKEELIQSATNSLGKPVPLKDVSVTINEVPLEDGYLTKQTGDFTIMYSYHDPYSLQ
                     ELHAQSLLHVLPLNKQIDQKEQKRREESLSEKKQQMAHAVSPTGKVKQTRSLELARIS
                     RKISESNKTIRQEAHLAPQVSGLENEILEPKIKQQSEKEFVAKQSEDQKESDDQKETK
                     KKRLSTIDSTDEHLQPQVPLPSPGTPAPGGGIPSSALGDFMRGMAGTWVYANRNEFDA
                     DDWF"
     gene            2311..2880
                     /locus_tag="SCJ86_001538"
     CDS             2311..2880
                     /locus_tag="SCJ86_001538"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327717.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEG1169079.1"
                     /translation="MLDKTLPYAEIWMTRPLQQNVPITPLAEGFEFRFYQKEDESAWA
                     EIETSVGEFQTTDEALEYFQQNFAPYPEELERRMFFVETKKGEKVATCTAWRKKSREQ
                     VYPVFHWLAVKPDYQGKGLAKALTARVLQEFPTLHTDGPIYLHTQTWSHQAIALYKKM
                     GFTFIAENLDGTPNPDYEKVMQVLSKLDK"
     gene            complement(3055..3861)
                     /locus_tag="SCJ86_001539"
     CDS             complement(3055..3861)
                     /locus_tag="SCJ86_001539"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEG1169080.1"
                     /translation="MNHLKTFFTNSTKTAGRITNGIYILLRIIVVIMLVDQVWNQHWG
                     NTMLLLLTLILFEIPYFLEKILKIQIPNMLEIILLSFIFSSTVLGELSDFYGYFSMWD
                     TFLHAFSGFLAGGVGFSLVYLLNKNTKTMNLTPLLLAVVSFCFSMTVGVMWEFVEFSA
                     DKLLQFDMQKDTIIQQINSVELSKDGNTVYHVPDITRTIIESQNADGKTDKTIIQNGY
                     LDVGIMDTMKDLFVNFIGAVVFSVFGYLYSRNDYQKFTFIKNFIPKKIKR"
     gene            3999..5513
                     /locus_tag="SCJ86_001540"
     CDS             3999..5513
                     /locus_tag="SCJ86_001540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016632010.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flavocytochrome c"
                     /protein_id="HEG1169081.1"
                     /translation="MKKLVAGLLTAVALLAMSACGADQKNTAQTSSSTKESAEVSSGA
                     SEQVYTDPKEMKESYDVVIIGAGGAGMAAAIEAKDAGLNPVILEKMPVAGGNTSKSSS
                     GMNASETKFQKEEGIKDSNDAFYEETLKGGGGTNDKEMLRYFVDHSADAVDWLDQNGI
                     KLDNLTITGGMSEKRTHRPSDGSAIGGYLVEGLLNNVHEREIPIFVNADVTDIKKNGE
                     TVNEVTVHVQGEEPKEVTGKAIVVTTGGFGASKEFIEKYRPDLADYITTNQEGSTGDG
                     ITMIEKLGGQTVDMDKIQIHPTVQQDEGVLIGEVVRGEGAILVDQEGKRFVNEMDTRD
                     KVSAAITALPEKSAYLIFDQGVRDRATAIEFYDQKGYVTTGKTMEELAEKIDLPKETL
                     AKTVDGWNTDVAEKKDAQFNRATAMEHDLSSPNYYAIKIAPGIHYTMGGVKINTNTEV
                     LDKENQPIRGLYAAGEVTGGLHGNNRIGGNSVAEIIIFGRQAGIQAARFASENE"
     gene            complement(5916..6899)
                     /locus_tag="SCJ86_001541"
     CDS             complement(5916..6899)
                     /locus_tag="SCJ86_001541"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373408.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-quinone oxidoreductase"
                     /protein_id="HEG1169082.1"
                     /translation="MRAISIKHPGSVDVLEQIEVPVPKPKQGELLIKVHTAAVNRTDI
                     MKRESVSLKPPYPILGVEVAGEVVENKSDISTFTPGTKVYGLVNLGGYAEYAVMPADR
                     AILLPDTLDYVSAAGISEVFLTAYQTLYWLGKLQEGETVLIHAGASGVGTAAIQLARQ
                     LSHAKIIVTAGSEEKLAFCKELGADELINYKKQDFQEEVRKITEGRGVDVILDFIGAS
                     YWEKNLASIAVDGRWVLIGMLGGTIVPEIDLGVLIGKRVQLLGTLLTPRSDQYKAELA
                     QTFMQKAGPFLENGKIRPIIDRTFQFSAVKQAHEYMEANKNIGKIILKITE"
     gene            complement(6904..7551)
                     /locus_tag="SCJ86_001542"
     CDS             complement(6904..7551)
                     /locus_tag="SCJ86_001542"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296930.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HEG1169083.1"
                     /translation="MKIFVVGANGQIGRHLIKDLAPSSHEIFAGVRDVASQTLVKKEN
                     VSYVSFDLTWSVEKMTEAFKGIDVLIFAAGSQGKNLLQVDLDGAIKTVIAAENAHVSR
                     YLMVSAVYADEPAKWPESMTDYYITKHYADEWLKRTNLDFVILQPVTLTNDEEVTSIQ
                     LTKPNEKASKTITRSTVAAVLAALVEETDISRTTLVLSEGSKELNTAFQEWAKEE"
     gene            complement(7636..8268)
                     /locus_tag="SCJ86_001543"
     CDS             complement(7636..8268)
                     /locus_tag="SCJ86_001543"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008268018.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ECF transporter S component"
                     /protein_id="HEG1169084.1"
                     /translation="MKNTKNFTLTAMFLAILILLAVTPLGFIPIGPINATTMHIPVII
                     ASIVLGPRLGAFLGGTFGLISMIRSTVIQTPLSFVFSPFIPVIGTEHGSWKALLIAFI
                     PRILIGVVPYFVYKGLRKLMKQKADSVSLFLAGLSGSLTNTILVMNMIYFLFQQDYAK
                     VIGTNLNAVYSAILAVIFTSGIPEGIVAGLATAAVCNVLLRYFKHPSLTN"
     gene            complement(8284..8835)
                     /gene="coaC"
                     /locus_tag="SCJ86_001544"
     CDS             complement(8284..8835)
                     /gene="coaC"
                     /locus_tag="SCJ86_001544"
                     /EC_number="4.1.1.36"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300740.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphopantothenoylcysteine decarboxylase"
                     /protein_id="HEG1169085.1"
                     /translation="MKKILLGVTGSISAYKSADLTSQFVKLGAQVDVIMSKSSTHFIT
                     PLTLQSLSKRAVHTDVMQESNPQVINHIELAKQADLFLIAPATANIIGKLANGIADDL
                     LSTVAMALPVGTPKLIAPAMNTNMYQNPITQRNLETLRSVGYQEIEPRESLLACGDFG
                     KGALADIQTIIEQVMLTLNERNA"
     gene            complement(8832..9569)
                     /locus_tag="SCJ86_001545"
     CDS             complement(8832..9569)
                     /locus_tag="SCJ86_001545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014386989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphopantothenate--cysteine ligase"
                     /protein_id="HEG1169086.1"
                     /translation="MKILITAGGTTEKIDQVRAITNHSTGRLGQALADYLAANPDTTI
                     DYVTTKQALKPKRHSNITIYTIESALDLFLQLEELTKKEHYDAIIHSMAVSDFTPAFS
                     FSEEQLAKNLPTSSTQEELDNWFAENEQTDTTVSKISSNTEHLVLVLKKTPKIISYLR
                     EWQPKAKIIGFKLLVDVPKESLLAVAKNSLINNKTDFIFANDLTEIHGETHHGYLLSK
                     DGTVEEAQSKSEIAALITEKIRLEESK"
     gene            complement(9566..10555)
                     /locus_tag="SCJ86_001546"
     CDS             complement(9566..10555)
                     /locus_tag="SCJ86_001546"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306123.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oxidoreductase"
                     /protein_id="HEG1169087.1"
                     /translation="MNAFRSFQVIKEPVFHTAISDLPFTELAENEVLVKIAYSDVNYK
                     DALAMSESGQVIRTYPMTPGIDLSGTVVQSNNPRFSKEDLVLATGFGLGVTHPGGYSQ
                     YQKVPGDWLVPLPKNMTLRQAMILGTAGFTAMLCVNALIRQGMTSDKKVVVTGASGGV
                     GSTAIALLHKLGFQSIIAFSRKEESVTWLKSLGALQVVRPDEFLPETTKALGKQQIDY
                     VIDTVGGEQLSSLLPLISYNGAVALCGNAGGIKLNATVLPFILRNIQLIGIDSVNVPI
                     DQRLSLWQQMADLQIADELVVQEITLDQLPETASKLLAGTHQGRTLVNVGDHK"
     gene            10813..11355
                     /locus_tag="SCJ86_001547"
     CDS             10813..11355
                     /locus_tag="SCJ86_001547"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002308415.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HEG1169088.1"
                     /translation="MEIGEKLRNLRIQKNLTQEELGERTDLSKGYISQLERDLSSPSM
                     ETFFSILEVLGVTPEEFFHQEAANLQVVYSKEDHTVYFDEENGYELEWLVADSNEKEM
                     EPVLLTFEGAGEYKTFEPSLSETFIFVLEGELELSLGETVYVAKAGQSIYYQATKQHQ
                     LRNHLKKRTKVLIVATQSYL"
     gene            11372..12457
                     /locus_tag="SCJ86_001548"
     CDS             11372..12457
                     /locus_tag="SCJ86_001548"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002356508.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEG1169089.1"
                     /translation="MGKPIISFDQVVKRYDDETVLKKVSFEIEQGKFYTLLGPSGCGK
                     TTILRIIAGFTEATEGDIYFEGKRLNDLPANKRQVNTVFQDYALFPHMNVFDNVAFGL
                     KIKKMSKSDIEKKVKDALRMVQLPGYEKREISEMSGGQRQRVAIARAIVNEPKVLLLD
                     EPLSALDLKLRTDMQYELRDLQQRLGITFIFVTHDQEEALAMSDEIFVMNKGKIVQSG
                     TPVDIYDEPINHFVADFVGESNIVDGVMIEDNLVEFVGKQFECVDGGMRLNEPVEVVL
                     RPEDLTITTPDKGKLVVTVDTQLFRGVHYEIICYDEQQNEWMVHSTKKAKEGSKVGLA
                     FEPEDIHVMRFNESEEEFDARLDSYEE"
     gene            12469..13275
                     /locus_tag="SCJ86_001549"
     CDS             12469..13275
                     /locus_tag="SCJ86_001549"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738847.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEG1169090.1"
                     /translation="MKTMRRIYSVPYILWLGLFVIAPVLMIIYQSFFDMNGQFTLNNY
                     LSYFTSGTSLSMTINSVWYAFLITFFTLIISYPTAYFLTKLKHKQLWLMLIILPTWVN
                     LLLKAYAFIGIFSVHGSVNHFFDFIGIGPKQILFTDFSFLFVATYIEIPFMIMPIFNA
                     LEEMNPSLISASRDLGANSFETFRRVIFPLLLNGVKSGVQAVFIPSLSLFMLTRLIGG
                     NRVITLGTAIEQHFLVTQNWGMGSTIGVILIIAMFIVMLLTGEKKKGGRS"
     gene            13276..14103
                     /locus_tag="SCJ86_001550"
     CDS             13276..14103
                     /locus_tag="SCJ86_001550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623353.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEG1169091.1"
                     /translation="MKKFKWSYLYLGIVFLFLYAPIFYLIFYSFNEGGTMNSFTGFTW
                     ENYEAVFEDTRLIIIVLNTFMLAFLSALIATIIGTFGAMGIYYTKKRRARTALLSMNN
                     ILLVSPDVIIGASFLIFFTLLGFGLGFQSVLLSHIAFSIPIVVLMVLPKLQEMNDSMV
                     DAARDLGANNFQVVKNIILPYLSPGIIAGYFMAFTYSLDDFAVTFFVTGNGFTTLSVE
                     IYSRARQGISLEINALSALVFLFSMILVIGYYFISKENRPKRLKKNRRVREGVARLR"
     gene            14100..15173
                     /locus_tag="SCJ86_001551"
     CDS             14100..15173
                     /locus_tag="SCJ86_001551"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293464.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HEG1169092.1"
                     /translation="MKKLQSLFLGILIIIAVLLLGVRQLEKTSGMSGAKVLTIYNWGD
                     YIDPSLITKFEKEYGYKVNYETFDSNEAMFTKIQQGGTNYDIAIPSEYMIQKMIKEKL
                     VLPLDHSKIKGLNNIDPRFLDLDFDPDNQYSIPYFWGTLGIVYNDKFFDANQIKHWDD
                     LWKPELKDSLMLIDGAREVMGLSLNSLGYSLNSKNMAQLTEAANKLNHLTPNVKAIVA
                     DEIKMYMINEEASVAVTFSGEAADMMWENEHLHYVIPPEGSNLWFDNIVMPKTVKNKE
                     GAYDFINFMLEPENAAQNAEYIGYSTPNEEAKALLPKDISSDEQFYPSDDTISHLEVY
                     KDLGSKYLGIYNDLFLEFKMYRR"
     gene            15601..18297
                     /locus_tag="SCJ86_001552"
     CDS             15601..18297
                     /locus_tag="SCJ86_001552"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317930.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma 54-interacting transcriptional regulator"
                     /protein_id="HEG1169093.1"
                     /translation="MEYKYRIDILQPIVYYAFRHIKLEEKMKKSAKDRVFDELIRLTG
                     NMSSVTAEKISKNLGISRQNASHYLTRLVEDKKVEKLPGKPVLWKPLDEYAVVDNTEQ
                     INEAFHSVVGHDGSLREVIQKCIAAVKYPPNGLSVLINGATGVGKSFLATKIFEYAIH
                     EQIIEKDAPFAILNCADYADNPELLSATLFGYKKGAFTGAEKDTDGLLATANNGYLFL
                     DEVHRLSKENQEKLFLFIDTGNYRPVGENVNWHSAKVRFIFATTEKGENYLLDTFDRR
                     IQISVMLPTFEDRPIRERLELIQLFFQNEADVLQKDIIVSREALSLMLSHPFSGNIGK
                     LKNIIKISCADVYSRTQEEPLTIGKNEILIQLNMLESEVESLPIASLLIKKNQKIKTD
                     RIKPFDYDSILEKITQLINEGNFNENFSSIKNEVQHLVHHIDKDSFLSMSRKLMIQMF
                     RKVWVSVLGKTYGLTTSGPVAEIISRSYVISPSYKTDLTGINRTLAANLPRTTYLCNQ
                     FLEHLPPLSEKDENFLRLLIVVTLSDYVDESIELKGLLLAHGDSTASSIQAVVNQLCG
                     NYVFEALDMPVESNVSEIVESTRNFVKRQVNTDSLVLIVDMGSLNQIYTQIKNDLKGE
                     LLLLNNLTTSIALDIGLKMTMNAPFKQIAEDAADKYSINVQYFEGFSQSSNIIISCMS
                     GLGISDKLKDVFRHHLSDESIEVFTKDYRELRELIDQNDETYFDKTKLVITTSDLPRS
                     FSIPNINVYDILDGDGAANLEHILSPEMKPAEFNRLIQELVKFFSLEGIADRLNFLNP
                     TIVMSEVETVISKYENYYHFTLNGRVKLNLYMHTALMMERLFLARTEKSEEPNYDLKP
                     DEDEFYYVSRSIFQSMEMKYNFKVNAYELSLLYELLGPYIQK"
     gene            18374..18862
                     /locus_tag="SCJ86_001553"
     CDS             18374..18862
                     /locus_tag="SCJ86_001553"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002391018.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HEG1169094.1"
                     /translation="MFLKVGTGIAYIKVNRKKGINHMKKILIATHGHLASGFLSSIQL
                     LTGKVQEITVINAYVDECDFEQELNVFVNQVNEQDQIFVFTDLFGGSVNQKITKTFLE
                     KEIAVTILSGFNLPILLEIVLATKELTKEDIQELVNKCQQELKMTILDEVHVVNEEED
                     FF"
     gene            18874..19368
                     /locus_tag="SCJ86_001554"
     CDS             18874..19368
                     /locus_tag="SCJ86_001554"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287026.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HEG1169095.1"
                     /translation="MIKAVRIDERLIHGQVAMTWTKSLNLTGLVVASDEAATNDIQKM
                     TLKMAAPSNVKVIIKTVDDAIHLLKDPRAKDMRLMVLVPNVKDAVKVAKAFPTEIELM
                     NVGNAGKMTPPSEDKKTLSKEVMLTTSEIQDLKELVELYPDTVFQPTPAMEKRSAISI
                     LSDF"
     gene            19389..20198
                     /locus_tag="SCJ86_001555"
     CDS             19389..20198
                     /locus_tag="SCJ86_001555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295012.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HEG1169096.1"
                     /translation="MYDAFVVALAVFIGIAGHEMFGMSMLSRPIVVAPLAGALMGDLQ
                     TGLIVGASLEAIFMGVVNIGISSTAEPSLAAGLATAFAIKMGGNVDAIIPIVFPLAVL
                     GLQLMNMIFSFVCGPMAAKFFKFAKSGDQKGIINLHFFMWFVHYALYALIPFFAVLFG
                     ADVVKSVLDNIPEVIMNGLTVAGNLLPAVGMAMLLRMLWSKNIAVWFFFGAVVMAYLE
                     LPLIAIAAVGTIIAIVIAQRDLQLKNISVHAVQSDAAVNATLDQEEEDFFQ"
     gene            20195..21016
                     /locus_tag="SCJ86_001556"
     CDS             20195..21016
                     /locus_tag="SCJ86_001556"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010755513.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS system mannose/fructose/sorbose family
                     transporter subunit IID"
                     /protein_id="HEG1169097.1"
                     /translation="MKLADGISKDEKKLVRKMFWRSMTMYASVNPVTMGGGGFAYSMM
                     PFINHFYKSEEDRAAALERHTQYFSTTIPCASFVMGIAASMEKENSEKAGFDVESINA
                     IKTSLMGPLAGIGDSLFWGVWRVVTAGLAINLANAGNILAPIIFLVMFNIPNYLMRYY
                     GGVLGYSLGSKYIEKLYANGLIEILTKAASILGLIMVGAMTISMVKFNTVLSFSMKGE
                     ELMNVQEILDSIFKGIVPLLVTLSCFKLLDKKVGVIKIIFGMVVLGILLSLLGIA"
     gene            21067..23166
                     /locus_tag="SCJ86_001557"
     CDS             21067..23166
                     /locus_tag="SCJ86_001557"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317929.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase"
                     /protein_id="HEG1169098.1"
                     /translation="MTINEVIYSPKHPFFNAELNDGNVTTSNEYSYYRNIPDVKGLTF
                     DVSNQKTLANLDVCGTIQTLTFYRENLLTEEKPGVWVNKQLSQTNSLSLKVEVNGIVY
                     HLSESDHRIEVDVIQDCLPRYIHHYSTFKVIVVSFAPILANKRLSMLVQQVYIVNETH
                     ESLQVSVKDVPSYQEKYSDQQNVLISQKGNKDTIAQQEVASFAIAFVDPNAFEEVMTF
                     QESDIEKWLKETLNYFAQLFGQLSMPNDRVVHLYRRALYQSFSSFGMNRQGQVVGSNW
                     GSYPATNRIWNKDMYYASLPFTMFEPELCQKTVLWFDQYGVKFPGSKFTGGINHSLSN
                     SLSSALLASLYFEHTGDTKFFERCPQVLLNASRIIEDILAQRRSGEPMLFPSVWLSDA
                     FALGKYHTGSNLCLWKACIGLAEIFEALEQLSLSKRYKNIACKLKESICKQMTIDGPF
                     GEQFLEGIGDEEKTTYSVKHYQKPILEQGLIFLSDVIVDGKINLLMHDGEESDTTLMP
                     FYQFLDNKDHLYQNTMAFSASSFNPTYSEEIKGITWGLESGATFPGFITVLMSELHNP
                     QAFVTRLEELICLADLDGSWWWWPYRLEAQQGEVVRDFGCGKCGWASGLFVSLMISQY
                     FGLKFKKGVLQIDPVDNLTFSWKNLKFGQAFISIECTQSELSISNLSEKPLKISDTKK
                     ILHVEKGKTIHIQRRKR"
     gene            23163..24281
                     /locus_tag="SCJ86_001558"
     CDS             23163..24281
                     /locus_tag="SCJ86_001558"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295018.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2961 domain-containing protein"
                     /protein_id="HEG1169099.1"
                     /translation="MSILKNITQFKKIQSRAITPENPTGEKGKAAMLPSELGPSRKGR
                     GAIPLTSGEETVIADITGTGIIRHMWMTIRDRTEFGSFVLRDLILRIYWDGSDSPAVE
                     SPLGDFFCNGFGERCEVNSLPIVVNPTGGFNSYFEMPFQTSAKITIENLHPKDMSSFF
                     YTINYALVDELEENSLYFHANWHRERITKLQEDYKLIDRLKGQGYYVGTYLALTCLER
                     YWWGEGEFKFYIDGDSEYPTVTSTGSEDYFGGAWAFHEYDEFGRPHAKNYSTAFLGYP
                     YQSNRDATRDRFETGKLNAVHAFGDDAMPRHGLYRWHLQDPICFTEDLSVTLQQIGND
                     DVRLFERQDDVASVAYWYSTEASGNTKRHFTDRERLPR"
     gene            24320..25198
                     /locus_tag="SCJ86_001559"
     CDS             24320..25198
                     /locus_tag="SCJ86_001559"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304270.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family protein"
                     /protein_id="HEG1169100.1"
                     /translation="MIYGAIEAGGTKFVCAVGTEELTILERVSFPTTTPVETMKQVID
                     FFKPFKEQLAGIGVGCFGPIDIQCKSTSFGHITSTPKLAWQNFNFVGTLKQYFNIPIE
                     WTTDVNAACYGEYVAGSGKGLAGVAYFTVGTGIGGGALFNGTFVEGFSHPEMGHMLIK
                     RHPKDNFSGNCPFHQDCLEGMAAGPAIEKRLGVKGQNLLADDSFWQIEAFYLAQCAYN
                     TTLMFSPDRIIFGGGVMKQEHMKKKVQDKFVELINGYVEIPPIDSYIITPELGDNAGI
                     IGGLALARKAVRNKQP"
     gene            25389..25541
                     /locus_tag="SCJ86_001560"
     CDS             25389..25541
                     /locus_tag="SCJ86_001560"
                     /inference="COORDINATES: protein motif:HMM:NF016526.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="accessory gene regulator B family protein"
                     /protein_id="HEG1169101.1"
                     /translation="MRIFTGGVHLNSNILCYFMSVVIVIVAYQCMFIFRNVDFIILGL
                     MFEFFW"
BASE COUNT         8166 a         4479 c         5439 g         7724 t
ORIGIN      
        1 aattaaaaag tacataagtt attattccga ttttcttgaa acaataaaaa atcaaaaaaa
       61 atactctcct aatagtccat cttcttttga ttttatttat ataatgaaat catcttatga
      121 aaattactac ttcatgttta gacaagagta gggaggacta agaataaatg tgttcaaaaa
      181 gacgttcaaa acaactaaat aaacacgctt gtcgtttaga taaggaacgg tcgtgttctg
      241 aaaaacacta cgttaagttt atgatgcgca cgatgctgat cagtttatct gtaggtgtct
      301 atatgcatgt acccgttcag gcaacgacac aaaccatcaa ggtcgtacgt acctctgttt
      361 ctgagaccac tgattcccct atcactatta ctcatacttt cactgatgca aatggaaaac
      421 agctacttcc tgcattgact tcctataata aagtcagtta tcctccaacg ataaaccact
      481 actggttaat tcctaacaaa acattatgga catcggaaac cggaagcagt gtgccgcttt
      541 ctatcttttc cgatatgact gatcctacaa aaggtgatca gttaaaacag atcactgata
      601 ttttgaatca tgctttgaca gatataaagg acggtggaag cgcacaagtt tcttatgtat
      661 atgaaaaaga ttattcacag tttgaaagaa agaaaatcga tgttctggca gggacaaaag
      721 tttcgatatc tgatctggta gaaaggctga agaacgcaga tggaacacaa ggagatatgt
      781 ccaaagtaat ttcaaacaat gggaatatcg tggatacgac taatacagga acatctacta
      841 tttccttgtc ctattttgat ccaattgctt taaaaacaat gactgcatct gctgtgatcc
      901 atgttcgtgc tgatcaaaca gctatcaaag gaaaagatct tcacttaaga acaggagaga
      961 gttacgcccc taaagatctt gttgaatatg taacaaaaag cgatggaact tcaggaaata
     1021 aagaagaaat caaaatgaac gggacagtag atactacaac tccgggagaa tatcccgttg
     1081 tattaactta tatagactcg aaaacatcaa atgtggcaga aacaacagct gtagtttatg
     1141 tagaagcatc tgctgcttca tcgactgtca tcactcattt tatagatagt caaacaaata
     1201 aagaacttca agcagaaaca catatgagta gtgtaaatcg tattcctgat ttgcgcctac
     1261 ctgctgaatt ttctgatcga gtgccaaata tagatttatc gacttatgaa caaaatggga
     1321 aagtcacttc tatttctgat tggatgcaag caaacggatt agacagaaaa agtcactggt
     1381 cggagatcct tgaagtactt cactcagaac tgatgaataa taggaagcaa tcaggagggg
     1441 aaattacctt tacttatgtt tatataccag acaatcgttc attagaaggt catgatctta
     1501 ttgttcatcc aaacgaaaaa ataaacaaag aagagttgat ccaatcagca acaaattcct
     1561 taggtaaacc cgtaccgtta aaggatgtat cggttacaat caatgaagta ccattggagg
     1621 atggttattt gaccaaacaa acaggtgatt ttacgattat gtatagttat catgacccgt
     1681 attcactcca agaactccat gctcagtcac tattacatgt tttaccatta aacaaacaga
     1741 tagatcaaaa agaacaaaaa agacgagaag aaagcttgtc agaaaaaaaa caacaaatgg
     1801 ctcatgcagt ttctccaaca gggaaagtaa aacaaacaag aagcctagag ttagcaagaa
     1861 tatctagaaa aatctcagaa tctaataaaa cgataaggca agaagctcat ttggccccac
     1921 aagtttctgg attggaaaat gaaattttag agcctaaaat aaaacaacaa tcagaaaaag
     1981 aatttgttgc taaacagtcg gaagatcaga aggaatcaga tgaccagaag gaaacgaaga
     2041 aaaaacgctt gtcaactatt gattccaccg atgagcatct gcagccgcaa gtaccacttc
     2101 ctagtccggg aacacccgca cctggtggag gtattccttc ttctgcttta ggagatttca
     2161 tgcgaggtat ggcaggaaca tgggtatacg ctaatcgaaa tgaatttgac gcagatgact
     2221 ggttctgatt tatatctcta tctgttatag tcaaataagt agctgagtca tatgttattt
     2281 ggcataatgg agacgaggag ggatagaaaa atgttagata aaacgttacc ttacgcagaa
     2341 atctggatga caagaccact tcaacaaaat gttccaatca cgcctttagc ggaaggtttt
     2401 gaatttcgat tttatcaaaa agaagatgaa tctgcttggg cggagattga gacttccgtt
     2461 ggggaatttc aaacgacaga tgaagcactt gagtattttc aacaaaactt tgctccttat
     2521 cctgaagaac ttgaaagaag gatgtttttt gtagagacaa aaaaaggaga aaaagtagca
     2581 acctgtaccg cttggcgaaa aaaatcaaga gagcaagtgt atccggtttt tcattggtta
     2641 gcagtgaaac cagattacca aggaaaaggg ctcgcaaaag cattgactgc cagagtcttg
     2701 caagaatttc ctaccctcca tacggacgga ccaatctatc ttcacacaca gacatggagc
     2761 catcaggcga ttgctcttta taaaaaaatg ggttttactt ttattgctga aaatctagac
     2821 ggcacaccta atccagatta tgaaaaagtt atgcaggttt taagtaaatt agataaataa
     2881 cgaaatggtg aaataagtcc tcagtattgg gataaaaaac gaaatagtca ttcctcatat
     2941 cttacttttt tgagttaata ctatcggata actaatggca tgccatacat tcaaaaaaga
     3001 aggggcagtg acaaaagtat tgtcactgcc ccttctcttg ttttttagca tgttttatcg
     3061 tttgattttt ttaggaataa aattcttgat gaacgtaaat ttttggtaat cgtttcgact
     3121 atagaggtaa ccaaatacac tgaaaacaac tgctccaata aaattaacaa ataaatcctt
     3181 catagtatcc atgattccca cgtctaagta accattttgg atgattgttt tatctgtttt
     3241 accatctgca ttttgacttt caatgattgt ccgagtgata tctggaacat gatagacagt
     3301 gtttccgtct ttacttaact ccacactatt gatttgctgg atgatcgtat ctttttgcat
     3361 atcgaattgc agtaatttat ctgctgaaaa ttccacaaac tcccacataa caccaactgt
     3421 catcgaaaaa cagaaggaaa caactgctag taataaagga gtcaagttca tggtttttgt
     3481 atttttattg agcaaataaa caagggaaaa gccgacaccc ccagctaaaa atccgctgaa
     3541 tgcatgtaga aaagtatccc acattgaaaa atagccataa aaatcactta actctcctaa
     3601 aactgttgaa ctaaaaataa aagataacaa aatgatttct agcatatttg gaatctgtat
     3661 ctttaatatc ttttccagaa aataagggat ttcaaataaa attaatgtca gtaataaaag
     3721 cattgtattt ccccagtgtt ggttccagac ctgatccact agcataataa cgacgatgat
     3781 acgtaataaa atatagattc cgtttgtgat tctacctgca gtttttgtac tgttcgtaaa
     3841 aaaggttttc aaatgattca taaaataact ccttcatgta tatccttaat tatactaaga
     3901 agcgtgtgtt taccaaagct atatgactta aagaaaaaca gagttgatat tgtgaaaaaa
     3961 atcacttaat atagtataag gagaaaaggg ggatacaaat gaaaaaatta gttgctggtt
     4021 tattaacagc cgttgcactt cttgctatga gcgcatgcgg cgcagatcag aaaaatacag
     4081 cgcagacaag ttctagtacg aaggaatctg ccgaagtatc atcaggcgct tctgaacaag
     4141 tatatacaga tccaaaagaa atgaaagaat cttatgatgt cgtgattatt ggagctggtg
     4201 gtgcagggat ggcagcagct atcgaagcaa aagatgctgg gttgaatcca gtaattttgg
     4261 aaaagatgcc tgttgccgga ggaaacacat ctaaatcatc aagcggtatg aatgcttcag
     4321 aaacaaaatt tcaaaaagaa gaaggaatca aagactcgaa tgatgccttc tatgaagaaa
     4381 cgctaaaagg tggtggagga acgaatgata aagaaatgct tcgatacttt gtcgatcact
     4441 ctgctgatgc ggtcgattgg ctagatcaaa acggtatcaa actggacaac ttaacgatca
     4501 ctggagggat gagtgaaaag aggacacatc gtccgtctga tggctcggct attggtggat
     4561 acttagtgga ggggttattg aataacgtcc atgagcggga aatccctatt tttgtcaacg
     4621 cagatgtgac agacattaag aaaaatgggg aaacggtcaa tgaagtgact gtacatgttc
     4681 aaggagaaga accaaaagag gtcaccggaa aagcaatcgt agttaccaca ggcggttttg
     4741 gtgccagcaa agaatttatt gaaaaatatc gtcccgattt agctgattat attacgacaa
     4801 atcaagaagg atcaactgga gatggtatca ccatgattga aaaattaggt ggacaaaccg
     4861 ttgatatgga taaaatacaa attcatccaa ctgtgcagca ggatgagggg gtcttgattg
     4921 gtgaggttgt tcgcggagaa ggagcaatct tggtcgatca agaggggaaa agatttgtca
     4981 atgaaatgga tacaagggat aaagtatctg cagcaattac agcattgcct gaaaaatcag
     5041 cttatttgat ttttgaccaa ggcgtgcgtg atcgtgccac agctattgaa ttttatgacc
     5101 aaaaaggata tgtcacaact ggaaagacaa tggaagaatt agcagaaaaa atcgatttac
     5161 caaaagaaac actagcaaaa acagttgatg gatggaatac ggatgttgcg gagaaaaaag
     5221 atgctcaatt caaccgtgcc acagccatgg aacatgactt atcgtctccg aattattatg
     5281 cgatcaaaat cgcaccagga atccattaca cgatgggcgg tgtgaaaatc aatacgaaca
     5341 ctgaagtatt agataaagaa aaccagccaa ttagggggtt atatgctgct ggtgaagtca
     5401 caggtggctt acatggaaac aaccggatcg gtggaaattc agttgcggag atcattattt
     5461 ttgggcgaca agcgggcata caagcagctc gatttgcttc ggaaaatgaa tgaaatagaa
     5521 cgtaaataaa aagaggttgt gaaatcagcg ttttgagcta ataccgaggg tcagctgatt
     5581 gaacactgtt tagtgaagag gttgtgaaaa agctgccctg catcaagtaa taagaacagc
     5641 caagtaaaaa cagcttctta tgtttttaac ttggttggac ttattaccgc agatgcaagc
     5701 ttttgaacac cgtttattcg gaaaaagagc gtgggacaaa agtaaagaac acttttgtct
     5761 cacgctctaa acacgtataa acggcggaag cagaagaaat tccttcggaa ataagctgaa
     5821 attcacaaaa atttgaaaag caattttcgt gaatttcttc ttatttcttg gagttaaacg
     5881 cttctgtccc agcctctttt ttgatcagca tgattttatt cagttatctt caaaatgatt
     5941 tttccgatgt ttttgtttgc ttccatatat tcatgcgctt gtttgactgc gctgaattgg
     6001 aatgtacggt caatgattgg ccgaatcttg ccattttcta gaaaaggccc agctttttgc
     6061 ataaaagttt gtgcgagctc tgctttatac tgatcacttc taggtgtcaa taatgtacca
     6121 agcagttgga cacgtttccc aatcaataca ccaaggtcta tctcaggcac gatggtccca
     6181 cctagcatac caatcaagac ccaacgtcca tcaacggcta tagaagctaa attcttttcc
     6241 cagtaagaag cgccgatgaa atccaaaata acatcgactc cccgaccttc agtgattttt
     6301 cgtacctctt cttgaaagtc ttgtttttta tagttgatca attcatctgc accaagttct
     6361 ttacaaaagg cgagtttttc ttctgatcct gcagtaacga tgattttcgc gtgagaaagc
     6421 tgtctagcta attggatcgc tgctgttccc acaccacttg ctcctgcatg gatcaagaca
     6481 gtttcgcctt cttgtaattt tccaagccag taaagtgttt gataagcagt caaaaaaact
     6541 tctgagattc cagcagcaga aacatagtct agcgtatcag ggagaaggat tgcacgatcg
     6601 gcaggcatca cagcatattc ggcataacca cctagattga ctaatccgta tacttttgtc
     6661 ccaggagtaa aagtggagat atcactttta ttctcaacta cctctccagc tacttcgact
     6721 cccaaaatag ggtagggagg tttcaaagag actgattctc ttttcataat atctgttcga
     6781 ttgacagctg ctgtatggac tttgatcagt aattcgcctt gttttggttt aggaacaggc
     6841 acttcaattt gttctaatac atctacagaa ccaggatgct tgattgaaat ggcacgcatg
     6901 gatctattcc tcctttgccc attcttggaa tgctgtattc aactctttgc taccttcaga
     6961 aagtactaga gtcgtacgag aaatatccgt ttcttccact agagcggcaa gtacagcagc
     7021 aactgtagag cgagtgatcg tttttgatgc cttctcatta ggcttagtta attgaatgga
     7081 agtaacttct tcatcgttag tcaatgtgac tggttgaaga atgacaaaat ctaagttagt
     7141 tcgtttcagc cattcatctg cataatgttt agtaatataa taatctgtca tggactcagg
     7201 ccattttgct ggttcatctg cataaacagc actgaccatc agatagcgag agacatgcgc
     7261 attttcagct gcgatcactg ttttgattgc gccatccaag tctacttgca gaagattctt
     7321 tccttgagag ccggcagcaa aaatcaatac atcgatgcct ttgaatgctt cggtcatttt
     7381 ttccacggac caagttaaat caaaagagac atacgaaaca ttctcttttt ttactaatgt
     7441 ttgggaagca acatctctca cccctgcaaa aatttcatgg gaacttggag ctaaatcttt
     7501 tatcaggtgt cggccaattt gtccgtttgc acctacaaca aaaattttca tttggatcat
     7561 cctttcgacg tcaaatcttt ccattcaata gtaacaccaa aagagaaaaa aatcagagat
     7621 cggctacgat gactatcagt tagtaagaga agggtgcttg aagtatctta acaagacatt
     7681 gcatactgct gcagtcgcaa gacctgcgac gattccttca ggtataccac ttgtaaaaat
     7741 aaccgctaga atagctgaat aaacagcatt taaatttgtt ccgatgactt tggcataatc
     7801 ttgctggaat aaaaagtaga tcatattcat cactaaaatc gtattggtca atgaaccgga
     7861 aagtccagct aagaataaag aaacggaatc tgctttttgt ttcattagtt tgcgcagacc
     7921 tttataaacg aaataaggaa ccaccccaat caagatgcga ggaataaacg cgatcagcaa
     7981 ggctttccag cttccatgtt ctgttccgat gactgggata aacggagaaa agacaaaaga
     8041 caaaggtgtt tggataactg tgctacggat catgctgatc agtccaaatg taccgccaag
     8101 aaaagcacct agtcttggtc ctaaaacgat cgaagcaatg attacaggaa tatgcatggt
     8161 tgttgcattg attggaccga tagggataaa acctaaaggc gtaacagcta ataaaatcaa
     8221 aatagctaaa aacatcgcag ttaatgtaaa gttttttgta tttttcataa gtactccttt
     8281 tttctaagca tttctttcat taagagtcag catgacctgt tcgatgatcg tctgaatatc
     8341 agctaaggca ccttttccaa aatctccaca agctaaaaga gattctcttg gttcaatttc
     8401 ctgatagccg acagatctca atgtttccag attacgctga gtgatcgggt tttgatacat
     8461 attcgtgttc atggcagggg caatcaattt tggtgttccc actggaagcg ccattgcgac
     8521 tgttgaaaga agatcgtctg caatgccgtt tgctagcttg ccgataatgt ttgctgtagc
     8581 aggcgcgatg agaaacaagt cagcttgttt tgctagttcg atatgattga ttacctgcgg
     8641 atttgattct tgcatgacgt ctgtatggac tgcgcgcttc gacagagatt gcaaggttaa
     8701 tggcgtgata aaatgtgtgc tgcttttaga cataatcaca tctacctggg cacctaattt
     8761 gacgaattga cttgtaagat cagcgctttt gtaagcagaa atacttccag taacgcctag
     8821 taagatcttt ttcattttga ttcctccagt ctaattttct cagtaatcaa tgcagcaatc
     8881 tcagatttac tttgagcttc ttccactgtt ccatctttag ataagagata gccgtggtgt
     8941 gtctcgccat ggatttccgt caaatcattt gcgaaaataa aatcagtttt gttatttatc
     9001 agactatttt ttgcgactgc cagaagagat tcctttggta catctactaa tagtttgaaa
     9061 ccaatgattt ttgcttttgg ctgccattca cgtagataag aaatgatttt tggtgttttc
     9121 ttcaaaacga gcacaagatg ctcggtgtta gaagagatct ttgaaacagt ggtatctgtc
     9181 tgttcatttt ccgcaaacca attgtccagt tcttcttgcg ttgaagaagt gggtaggttt
     9241 tttgccagct gttcttctga aaaactaaaa gcaggagtaa aatcactgac tgccatactg
     9301 tggataatcg catcgtagtg ttcttttttc gttagttctt ctaattgtaa aaataagtcc
     9361 agtgcagatt cgatcgtata aatcgttata ttggagtgtc ttttcggttt cagtgcttgt
     9421 ttcgtagtca cgtaatcgat tgtggtatcc ggattggcag ctaaataatc agctaatgct
     9481 tgccctaatc gtcctgtcga atgatttgta atagcacgta cttgatcgat tttttcagtc
     9541 gttcctccag ctgtgattag aatcttcatt tatggtcacc tacatttact agtgttcttc
     9601 cttggtgtgt tccagcaagt agtttagatg ctgtttcagg caattgatcc agagtaatct
     9661 cttggacgac cagttcatct gcaatctgca aatccgccat ctgttgccat aaagaaagac
     9721 gttgatctat tggtacattg acagaatcaa ttccaatcaa ctggatgttt cgcagaataa
     9781 aaggcaagac agtcgcgttt agtttgattc ccccagcatt gccacataaa gctactgcac
     9841 cgttataaga gatcaatgga agaagactgc ttagctgctc gccacctact gtgtcgatga
     9901 catagtcgat ttgctgtttt cctaaagctt ttgttgtctc tggcagaaat tcatcaggtc
     9961 gtacgacttg caaagcacct agacttttta gccatgtgac agactcttct tttctggaaa
    10021 aggcaatgat tgactggaat cctagtttat gtaacagggc tatcgctgta ctgcctacac
    10081 cgccagaagc accagtaacg acaacttttt tatcggaggt cataccttga cggattaaag
    10141 cgttcacaca caacatagca gtaaaccctg ctgtaccaag gatcatcgct tgacgcagcg
    10201 tcatattttt tggtagagga actaaccagt ctcctggtac tttttgatat tggctataac
    10261 cgcctggatg ggtgacgcca agcccaaaac cagtagcaag tacaagatct tcttttgaaa
    10321 aacgtggatt gttactttgg acgacagttc cggataaatc aatccctgga gtcatcggat
    10381 atgttcgtat cacctgacca gactcgctca ttgccaatgc atctttataa ttgacatcag
    10441 aataggcaat ttttaccaag acttcatttt cagccaactc agtaaatggc agatcactga
    10501 ttgctgtatg gaaaacaggt tcctttatta cctgaaaaga tcgaaaagcg ttcattcgtg
    10561 agacactcct tttaaaattt tcttttatca taccataaag cctactcttt tattcttgtt
    10621 cttccgtaaa tttagacaaa aattaggtga aaaataaaga atcaaacaac tagaatttat
    10681 tccaaaagat aaagaaatat tcagcaaaaa aaattttaca aagggttgcc tttttcaata
    10741 aatttcgtat actaatacga gtggtagtaa acaggaagtt tactattaga aaacttcgat
    10801 tcggaggaaa ttatggaaat tggtgagaag ttaagaaatt tgcgcatcca aaagaatttg
    10861 acacaagaag aactaggcga acgcacagat ttgtcaaaag ggtatatttc ccagttggaa
    10921 cgagatttaa gttcaccatc aatggaaaca ttcttttcta ttcttgaagt cttaggtgtg
    10981 actccagaag aattttttca tcaagaagca gcgaatctgc aagtggttta ttcaaaagaa
    11041 gatcatacgg tttatttcga tgaagaaaac gggtatgaat tggaatggct cgtggctgat
    11101 tcgaatgaaa aagaaatgga gcctgtcttg ctgacttttg aaggtgcagg agaatataag
    11161 acgtttgaac cttcattatc tgaaacgttt atttttgtat tagaagggga attagagtta
    11221 agcttaggag agacggtcta tgtggcaaaa gcaggacagt cgatctatta tcaagcgaca
    11281 aaacaacatc aattaaggaa tcatctcaaa aaacgaacaa aagtattgat tgttgcaacg
    11341 caatcatatt tgtaatatag ggggtaaaaa cgtggggaaa ccaatcattt catttgatca
    11401 agtagtgaaa cgctatgatg atgaaacagt attaaaaaaa gtaagttttg agatcgaaca
    11461 aggaaaattt tatacactgc ttggtccttc cggctgtggt aaaacaacga tcctacgtat
    11521 tatagctgga ttcaccgaag ctacagaagg ggacatctat tttgaaggga aacggttgaa
    11581 tgatttgcca gctaataagc gacaagtaaa tacagtcttc caagattatg cactttttcc
    11641 tcatatgaat gtttttgata acgttgcttt tggattgaag atcaagaaaa tgtccaaatc
    11701 agatatcgaa aagaaggtaa aagatgcttt gcgtatggtc cagcttcctg gatacgaaaa
    11761 acgggagatt agtgaaatgt ctggtggtca aagacagcgt gtagcgattg cacgagcaat
    11821 tgtaaatgaa ccgaaagtat tgctattaga tgaaccgtta tcggcgcttg atttgaaact
    11881 acgtacagat atgcagtatg aactgcggga tctgcaacaa cgattaggaa tcacgtttat
    11941 ttttgttact catgaccaag aagaagcgtt ggcaatgagt gatgagattt ttgtcatgaa
    12001 taaagggaaa atcgtccaaa gtggtacacc cgtagatatt tatgatgagc caatcaatca
    12061 tttcgttgct gattttgttg gggagagcaa tatcgtggac ggtgtaatga tcgaagataa
    12121 tctagttgag tttgttggta agcaatttga atgtgtcgat ggcgggatgc gtctaaatga
    12181 accagttgaa gttgttttac gcccagagga tttgacgatc acgacaccag ataaaggaaa
    12241 actagtcgta acggtcgata ctcagctgtt tcgtggtgtc cattatgaaa tcatctgtta
    12301 tgatgaacag caaaatgaat ggatggtcca ttcaacgaaa aaagctaaag aaggaagcaa
    12361 agtaggattg gcctttgagc cagaagatat ccatgtcatg cgtttcaacg agtctgaaga
    12421 agaattcgat gcacgtcttg acagctacga agaatagggg ggacaagaat gaaaacaatg
    12481 cgcaggatct attccgtccc ttatattttg tggctcggtt tattcgtgat cgcgcctgta
    12541 ttaatgatca tttatcaatc tttttttgat atgaatgggc aattcacgct gaataattat
    12601 ctatcttatt ttacgtcggg gacatcgctt tctatgacga tcaattctgt atggtatgca
    12661 tttttgatca cgtttttcac gctgatcatc agttacccga ctgcttattt tttaacaaaa
    12721 ctgaagcaca agcaattatg gctgatgctg attattttgc ctacatgggt gaatctgctg
    12781 cttaaagctt acgcatttat tgggattttc agtgtgcacg gcagcgtgaa tcatttcttt
    12841 gattttatcg ggatcgggcc gaaacagata ttattcacag acttcagttt tctgttcgtg
    12901 gcaacgtata tcgagattcc atttatgatt atgccaattt tcaatgcatt ggaagagatg
    12961 aatccttctt tgatcagtgc tagtagagat ttaggcgcga atagctttga gaccttccga
    13021 cgagtgattt ttccgttatt attaaatggt gtgaaaagtg gtgtccaagc agtatttatt
    13081 ccttctcttt ctttattcat gctgactcgt ctgattggcg gaaatcgggt gatcacttta
    13141 ggaacggcta tcgaacagca tttcttggtt acacaaaact gggggatggg ttctacaatc
    13201 ggtgtgattt taatcattgc tatgttcatc gtgatgctcc tgacaggcga gaagaaaaaa
    13261 ggaggtcgct cataaatgaa aaaatttaaa tggtcgtatc tttatttagg gatcgtcttt
    13321 ttattcttat atgcaccgat cttctatctg attttttatt ccttcaatga aggaggaacg
    13381 atgaacagct ttacaggatt tacttgggaa aattatgaag ctgtttttga agatacacgt
    13441 ttgatcatta tcgtattgaa tacgttcatg ctggctttct tatctgcatt gatcgccacg
    13501 atcatcggga catttggggc tatgggaatc tattacacga aaaaacgacg tgcacggaca
    13561 gcgttgttga gcatgaataa catcttatta gtctctcctg atgttattat cggtgctagt
    13621 ttcttgattt tctttacact actaggattc ggattaggtt ttcagagtgt attgctttcc
    13681 catattgctt tcagcattcc aatcgtcgtc ttgatggtct tgccaaaatt gcaggaaatg
    13741 aacgattcaa tggtcgatgc ggctagagac cttggtgcta ataatttcca agtagtcaaa
    13801 aatatcattt tgccttatct ttctccagga atcatagctg gatattttat ggcatttacc
    13861 tattctcttg atgattttgc ggttacattt ttcgtcacag gaaatggctt tacgacgctc
    13921 tcagtagaga tctattctcg tgcaagacaa ggaatcagtt tagaaatcaa tgcgctaagt
    13981 gcgttagttt tcttgttctc catgatattg gtcattggat actactttat tagtaaagaa
    14041 aaccgtccaa aacggctgaa gaaaaacaga agagtacgcg agggggtggc acggctgcga
    14101 tgaaaaaatt acaatctctg tttttaggta ttctcatcat tattgcggtg ctgctgcttg
    14161 gtgttcgtca acttgaaaaa acaagtggca tgagtggagc aaaggtactt accatctata
    14221 actggggaga ttatatcgat cctagtttga tcacgaaatt tgaaaaagaa tatggataca
    14281 aagtgaatta tgaaacattt gattctaatg aagcgatgtt cacaaaaatc cagcaaggtg
    14341 ggacaaacta cgacatcgct ataccaagtg aatatatgat ccaaaaaatg atcaaagaaa
    14401 aactagtact tccattagat cattcgaaga tcaaaggatt aaataatatt gatccacgat
    14461 ttttagactt ggattttgat ccagataatc agtattcgat tccatatttt tggggaactt
    14521 tagggatcgt atataacgat aaattctttg atgcaaatca aattaagcat tgggatgatt
    14581 tgtggaagcc ggaattaaaa gacagtttga tgctgattga cggtgcacga gaagttatgg
    14641 ggctttcttt gaatagttta ggctattctt tgaatagtaa gaatatggca cagttgacag
    14701 aagcagcaaa taaattgaat catttaacac caaatgtcaa agcaatcgtg gcagatgaga
    14761 tcaaaatgta tatgatcaat gaagaagcct ccgtggcagt gacattttct ggcgaagctg
    14821 ccgatatgat gtgggaaaat gaacatttac attatgtgat cccaccagaa ggatccaatc
    14881 tttggtttga taatatcgtt atgccaaaaa ccgtgaaaaa taaagaaggt gcctatgatt
    14941 tcatcaactt tatgctagaa ccggaaaatg cggcacaaaa tgcagagtat attggttatt
    15001 ctacaccaaa tgaagaagca aaagcgttat tgccaaaaga catttcgtcg gacgaacaat
    15061 tctatccatc tgatgacacg atctcccatc ttgaagtcta caaagatcta ggatcgaaat
    15121 acctaggcat ctataatgac ctattcttag aattcaagat gtataggaga taatggcaaa
    15181 actcagtaag aatggggact tatcaacgtt ctaaagagct aaaaatcgtc ctttttctat
    15241 ctgagttttc caatcttcta ccaaaagcgg atgaaacgcc gctttttttg tgttatttta
    15301 ttttttctac cacgtttttc taccagagaa aagtggacaa aaccattgta aaatagggcc
    15361 cgagacgggc tagtaaatct aagattgaaa aagttacaga aggataaagt aattggaaac
    15421 tatgcaaata tacttaaccc ttctgtaatc tattaataaa actaatatat gaatccagtt
    15481 caatatttgt agctatgaaa aaacataaaa atcatattag ataaaaacta aattggtaga
    15541 taaattattt tgtcagatat ctgaggcaaa tattcgacta tttgtgtttc aaagtaaaaa
    15601 ttggaataca aatatagaat agacatattg cagccgatag tttattatgc ttttagacac
    15661 attaaactgg aggaaaaaat gaaaaagagc gctaaagatc gagtttttga tgagctgata
    15721 cgactaacag ggaatatgtc ttctgtcact gccgagaaaa tttcgaagaa tctaggtatc
    15781 tctcgtcaga atgccagcca ttatttgacg agattagtgg aagataaaaa ggtcgagaaa
    15841 ttaccgggaa agcctgtttt gtggaagcct ttagatgaat atgcggttgt agataatacg
    15901 gagcaaataa acgaagcctt tcattctgta gttggtcatg acggaagtct gagagaggtc
    15961 attcaaaaat gtatagccgc tgtcaaatat ccgcctaacg gactcagtgt attaataaat
    16021 ggagccacgg gtgttggaaa aagtttttta gccacaaaaa tttttgaata cgctatccat
    16081 gagcaaatta ttgaaaaaga tgcaccattt gctatcttaa actgtgccga ttatgcggac
    16141 aatcccgaac tgctttcggc aacactgttt ggatacaaga agggggcctt tacgggggct
    16201 gaaaaggata ccgatggttt attggcaacg gccaacaacg gctatctttt tctcgatgag
    16261 gtccatcgcc tatcgaaaga aaatcaagaa aagctctttc tatttattga tactggaaac
    16321 tatcgtccag tcggtgaaaa tgtaaattgg cattctgcta aggttcgttt tattttcgcc
    16381 acgacggaaa aaggtgaaaa ttatttatta gacacctttg atcggcgtat tcaaattagt
    16441 gttatgttgc caacttttga agatcgcccc attcgagaac gattagagct gattcaatta
    16501 ttttttcaaa atgaagcgga tgttttacaa aaagatatta ttgtttcaag agaagccttg
    16561 tctttaatgt tatctcatcc ctttagtggg aatattggta agctgaagaa tatcattaaa
    16621 atcagttgtg cggatgtgta tagtcgaaca caagaagaac cattaacgat tgggaaaaat
    16681 gagatcttga ttcagttaaa tatgctggaa tccgaagtag aaagcttgcc gattgctagt
    16741 ttattgataa agaaaaatca aaagataaaa acggatcgaa tcaaaccgtt tgattatgat
    16801 tcgattttgg aaaaaattac tcagctgata aatgaaggta atttcaatga aaatttttct
    16861 tcgataaaga atgaagtaca gcatttggtg catcacattg ataaggattc ttttttatca
    16921 atgagtagaa aattgatgat tcagatgttt agaaaggtct gggtatccgt tttaggaaaa
    16981 acgtacggat tgacgacgag tggacctgtt gcagaaatta ttagcagatc ctatgtcatt
    17041 tccccaagct ataagacaga cttaaccggg ataaatcgta cattagctgc aaatctacct
    17101 cggacgactt acttatgtaa tcagttcttg gagcatttac caccgctctc agaaaaggac
    17161 gagaactttc taagactgct tattgtcgtg actttatctg attatgtaga tgaatccatc
    17221 gagctgaagg gcctgttgct tgctcacgga gatagtacgg ctagcagcat tcaagcagta
    17281 gtgaatcaat tatgcggcaa ttatgttttt gaagcgttgg atatgccagt agagagtaat
    17341 gtgtcagaga ttgtcgaatc gacaaggaat ttcgtgaaac ggcaagtgaa tacagatagt
    17401 ctagtgctga tcgtagatat gggctcttta aatcaaattt acacacagat caagaacgat
    17461 ttaaaagggg agttacttct tctgaataat ttgacgactt ccattgcact tgatattggc
    17521 ttgaagatga cgatgaatgc gccatttaaa cagattgcgg aggatgcagc agacaagtat
    17581 tcaatcaatg ttcaatactt tgaaggcttt tctcaaagta gcaacattat catttcctgt
    17641 atgtcgggtt tggggatttc ggacaaattg aaagatgttt ttcgtcatca tttatcggat
    17701 gaatccatcg aagttttcac gaaggactac agagaactac gtgaattaat cgaccaaaat
    17761 gatgaaactt attttgacaa gacaaaattg gtcatcacga cttcggatct tccacgttcc
    17821 ttttcaattc ctaatattaa tgtttatgac atcttagacg gtgatggagc agccaatttg
    17881 gagcatattt tgagtcctga aatgaagccg gcagaattta accgcttgat tcaggagcta
    17941 gtaaaattct tttcgttgga aggaattgcg gaccgtttga actttctgaa cccaacgatc
    18001 gtgatgagtg aagtggaaac agtcatttct aaatatgaaa attactatca tttcacttta
    18061 aatggccgtg taaaactaaa tctctatatg catacggcat tgatgatgga acgtttattc
    18121 ttggctcgga cagaaaaatc tgaagaaccg aattatgatt tgaaaccaga tgaagatgaa
    18181 ttttattatg tttctagaag tatttttcaa tcaatggaga tgaaatataa ttttaaagtc
    18241 aatgcttatg agctttcgct gctctatgaa ttattaggac cgtatattca aaaataaagt
    18301 gtctaaaaaa gtgtctaatc tgtttttttt aggttagaca ctttttttaa tgtgaattaa
    18361 cgtgattaaa ccaatgtttt taaaagttgg cacaggaatt gcttatataa aagtgaacag
    18421 aaagaaagga attaaccata tgaagaaaat tctaatagcg actcacggtc acttagcttc
    18481 aggctttctt agcagtattc aattactgac ggggaaggtt caagagatta cggtgattaa
    18541 tgcttatgtg gatgagtgtg attttgaaca ggagttgaat gtatttgtaa accaggtgaa
    18601 cgagcaggat cagatttttg tttttaccga tttatttggc ggaagtgtca atcagaagat
    18661 taccaaaacc tttttggaga aagagattgc tgtaactatt ttgtctggct ttaatctgcc
    18721 aattctgctc gaaatagtat tggcaaccaa ggaattaaca aaggaggata ttcaggaact
    18781 ggtaaataaa tgccaacagg aattaaagat gaccattctt gatgaggtac atgtagtaaa
    18841 tgaagaggaa gatttttttt aggaggaaga aaaatgatta aagcagtgcg aattgatgaa
    18901 cgattaattc atggacaagt agcgatgact tggacaaaga gtctaaattt aaccgggtta
    18961 gtagtagcaa gcgatgaggc ggctaccaac gatattcaaa agatgacatt gaagatggct
    19021 gcaccatcga atgtcaaagt cattattaaa acagttgatg acgcgattca tttgttaaaa
    19081 gatccgcggg cgaaggacat gcgcttgatg gttttagtcc ctaatgtcaa agatgcggta
    19141 aaagttgcga aagctttccc aacggaaatc gaattaatga acgtggggaa tgctgggaaa
    19201 atgacaccgc catcggaaga taagaaaacc ttatcaaagg aagtgatgtt aacgacgtca
    19261 gaaattcaag acttaaaaga attagttgaa ctttatccag ataccgtttt tcagcctaca
    19321 ccggcgatgg aaaaacgttc agctatctct attttgagtg atttttagaa aaaggaagga
    19381 gaaaaattat gtatgatgca tttgtagtcg ctttggccgt ttttattgga attgctggac
    19441 atgaaatgtt cgggatgtcc atgctgagtc gtccgattgt tgttgctccg ttagcaggag
    19501 cgttgatggg ggatttgcag acggggttga ttgtcggggc gtcactagaa gccatcttta
    19561 tgggtgtcgt caatatcgga atctctagta cggcggagcc ttctttggct gctgggctgg
    19621 ctacagcgtt cgcaatcaaa atgggaggaa acgtggatgc gattattccc attgtctttc
    19681 cactagcagt attaggatta cagttaatga atatgatttt ttcttttgta tgtggaccga
    19741 tggctgctaa atttttcaaa tttgcaaaat ctggtgatca aaaaggaatc atcaatctgc
    19801 acttctttat gtggtttgtc cactatgcgc tttatgctct tattccattc tttgccgttt
    19861 tatttggagc agatgttgtc aaaagcgtct tggataatat tccagaggtc atcatgaacg
    19921 gattaacagt cgctggaaat ctgctgccgg cagttgggat ggcgatgctg ttacggatgc
    19981 tttggagtaa aaatattgct gtgtggtttt tctttggggc cgttgttatg gcgtatctcg
    20041 agttaccatt gattgcaatt gccgcagttg gaacgattat tgcaattgtt atagcccaaa
    20101 gagatctaca actaaaaaat atatctgttc atgcagttca aagcgatgct gctgtaaatg
    20161 caacgcttga ccaagaagag gaggatttct ttcaatgaaa ttagctgatg gaatttctaa
    20221 agatgagaaa aaattggttc ggaagatgtt ttggcgctcg atgactatgt atgcttcggt
    20281 gaatccggtg acgatgggcg gcggtggttt cgcctattct atgatgccct tcattaatca
    20341 tttctacaaa agtgaagaag atcgagctgc ggcgctggaa cgtcatacac agtatttttc
    20401 gacgaccatt ccttgcgcgt cctttgtcat ggggattgcg gcatcgatgg agaaggaaaa
    20461 ttctgaaaaa gctggttttg acgtggaaag tatcaacgcg atcaaaacga gtttgatggg
    20521 accattggct ggaattggcg attcattgtt ctggggagtt tggcgtgtcg ttaccgcggg
    20581 actagcgatc aacttagcca atgccggaaa tattttggca ccaattatct ttttagttat
    20641 gttcaatata cctaattact tgatgcgata ctatggcggc gttttaggct attcattagg
    20701 ctctaaatat attgaaaagc tgtatgcgaa cgggttaatt gaaattctga caaaagctgc
    20761 tagtatcttg ggtctgatca tggtaggtgc aatgacgatc agtatggtca aattcaatac
    20821 cgttttgagc ttttcaatga agggcgaaga actaatgaat gtccaagaaa tcctcgatag
    20881 tatttttaaa ggaattgtac cgttattggt aacattgagc tgtttcaaat tactagataa
    20941 aaaagtcggc gtcatcaaaa ttatttttgg aatggtcgtc ttgggaattt tattgtcatt
    21001 acttggaatc gcatagtagg aagctgcagc tactaggctg cagttttttt agcaaggagg
    21061 gaaaagatga ctattaatga agtaatttat tcacccaaac acccattttt taatgctgag
    21121 ttaaacgacg gaaatgtgac tacttcaaac gaatacagct actatcggaa tattcctgat
    21181 gtaaagggat tgaccttcga tgtaagtaat caaaagacat tagcaaacct tgatgtttgc
    21241 gggacgattc aaacgttgac cttttatcga gaaaatttac taactgagga aaaaccaggt
    21301 gtttgggtca ataaacagct ctctcaaacc aattcgttat ccttaaaggt tgaagtaaac
    21361 gggattgtct accatttgtc agaatcggat catcgtattg aagtcgatgt gattcaagac
    21421 tgtttgccta gatatattca tcattattcg acgtttaagg tcattgtagt atcctttgct
    21481 cccatactgg ctaataagcg tttaagtatg cttgttcaac aggtttacat tgtgaatgaa
    21541 acacacgaaa gccttcaagt ttcagtgaaa gatgtgcctt cgtatcaaga gaagtattcg
    21601 gatcagcaaa atgtgttgat ttctcaaaaa ggaaataagg acacgatcgc acaacaggaa
    21661 gtggcaagct tcgccattgc ttttgtggac ccaaatgctt ttgaagaagt aatgactttt
    21721 caggaatctg atatcgaaaa atggctgaag gaaacattga actattttgc tcagctattt
    21781 gggcagctgt caatgccaaa cgatagagtt gtccatctct atcgtcgtgc actttatcaa
    21841 agcttttcta gtttcggtat gaatcgacag ggacaggtcg tcggcagcaa ttggggcagc
    21901 tatcctgcta ctaatcgaat ttggaataag gatatgtatt acgcatcatt gccattcacc
    21961 atgtttgaac cggagttatg tcaaaaaacg gttctatggt ttgatcaata tggcgttaag
    22021 tttcctggtt caaaatttac aggaggtatc aatcattctc tgagtaattc gttatccagt
    22081 gcgttactgg ctagtcttta ttttgaacat actggggata caaaattctt tgagcggtgt
    22141 ccgcaggttc tactgaatgc ttcaagaatt attgaggaca tattagctca acgtcggtca
    22201 ggggaaccaa tgctttttcc atctgtttgg ttatcagacg cctttgcact tggaaagtat
    22261 cataccggca gcaatctttg tctttggaag gcctgtattg gacttgcaga aatttttgaa
    22321 gccctagaac aactgagtct tagcaagcga tacaaaaaca ttgcctgtaa actaaaggaa
    22381 tcaatttgta aacaaatgac tatcgatggt ccttttggtg agcaattttt ggagggaatc
    22441 ggtgatgagg agaaaacaac ctattcagtg aagcactatc aaaaaccgat tcttgagcaa
    22501 gggttaatct ttttaagtga tgtgattgtg gatggaaaaa ttaatttatt gatgcatgat
    22561 ggagaagaat cagacactac gttgatgcct ttttatcaat ttttagataa taaagatcat
    22621 ttatatcaga acaccatggc cttttctgct agttctttca atccaaccta tagcgaggaa
    22681 atcaaaggca tcacttgggg attagaatcc ggcgccactt ttcctggctt tatcactgtt
    22741 ttaatgagcg agctgcacaa ccctcaagct ttcgtaaccc gattagaaga actaatttgt
    22801 ttggcagatt tagacggttc gtggtggtgg tggccgtatc gcttagaggc tcaacaagga
    22861 gaagtcgtgc gagactttgg ctgcggcaaa tgcggctggg catcgggtct ttttgtcagc
    22921 ctaatgatca gtcaatattt cggattgaaa ttcaaaaaag gagtattgca aattgatcct
    22981 gttgataatt taacgttttc ttggaagaat ttgaagttcg gtcaagcctt catttcgatt
    23041 gaatgtacgc aatccgagtt gagtatttca aatttgtcag aaaagccatt gaaaataagt
    23101 gatacgaaaa aaattcttca cgtagaaaag ggaaaaacga ttcacattca aaggagaaaa
    23161 agatgagtat actaaaaaat attacccaat ttaaaaaaat ccagagtcga gcaatcactc
    23221 ccgaaaatcc aacaggcgag aaaggtaaag ctgcgatgtt gccaagcgag ctaggaccaa
    23281 gccgcaaagg tcgtggcgca attccgctga cttctggtga agaaactgtg attgcggata
    23341 ttactggaac gggaataatt cgtcatatgt ggatgacgat tcgtgatcga acagaatttg
    23401 gcagttttgt tttacgtgat ttaatcttaa gaatttattg ggatgggtcg gatagccctg
    23461 ctgttgaaag tcctttaggg gatttctttt gtaatgggtt tggagagcgt tgcgaggtga
    23521 actcgttgcc gatagtggtt aatcctacag ggggctttaa ttcctatttc gaaatgcctt
    23581 tccaaacatc cgctaaaatt acgatcgaga atttacatcc gaaggatatg tcctcatttt
    23641 tttatacgat aaattacgct ttggtggacg aattggaaga aaatagccta tattttcacg
    23701 caaattggca tcgggaacgc attaccaaat tacaggagga ttataagtta attgatcgat
    23761 taaagggtca aggctattac gtgggtactt accttgcgct tacctgccta gaacgttatt
    23821 ggtggggcga aggcgagttt aaattctaca ttgatgggga tagtgagtat ccgaccgtaa
    23881 cctcaacagg ttcagaagat tattttggcg gggcgtgggc ctttcatgag tacgatgagt
    23941 tcggacgtcc acatgcgaaa aactattcaa cagccttttt agggtatcct taccaatcaa
    24001 atagagatgc cacaagagat cgttttgaaa cgggaaaatt gaatgccgtt catgcctttg
    24061 gtgacgacgc aatgccacga catggtcttt atcgttggca tcttcaagac cccatctgtt
    24121 ttaccgaaga tttatcggtc actttacaac aaatcggcaa cgatgatgtt cgcttatttg
    24181 agcggcaaga cgatgttgct agtgtcgctt actggtattc aacagaagct tcaggaaata
    24241 ctaaacgtca tttcactgat cgagaacgct tgccgcgtta atgaagtgtt tttcacatat
    24301 tcgaagggag gaacctatga tgatatatgg ggcaattgaa gctggaggga ctaaatttgt
    24361 ctgtgcagtc ggtactgagg aattaacgat tttggaaaga gtcagttttc ccacaacaac
    24421 tccagttgaa acaatgaagc aggtcattga tttttttaaa ccttttaagg aacaattggc
    24481 aggcatcggg gtcggctgct ttggtccaat tgatattcaa tgcaaatcaa cgagtttcgg
    24541 tcatatcaca tcaacaccaa aacttgcttg gcaaaatttc aattttgtag gaacgctaaa
    24601 gcaatatttc aatattccga ttgaatggac aacagatgtc aatgcggctt gctatggtga
    24661 atatgttgct ggttctggca aagggttagc aggtgttgca tacttcactg tgggaacagg
    24721 tatcggaggt ggtgccttgt tcaatgggac tttcgtggaa gggttcagtc atccggaaat
    24781 ggggcatatg ttgatcaagc gacatccaaa ggacaatttt tcaggaaact gtcccttcca
    24841 ccaagattgt ttagagggga tggcagcagg accagcgatt gaaaaacggt tgggtgtaaa
    24901 aggccagaat ttattggctg acgattcgtt ttggcagatt gaagcctttt accttgccca
    24961 atgtgcatac aatacaacac tgatgttttc gccagataga attatttttg gcggtggtgt
    25021 aatgaaacaa gaacacatga agaaaaaagt tcaggataaa tttgttgagc ttataaatgg
    25081 atatgttgaa ataccaccaa ttgatagcta cattattacg cccgaattag gtgataatgc
    25141 cggaataatc ggcggattag cattggccag aaaagcggta agaaataagc aaccataatt
    25201 aatgaatgtt tcattgatac tcatgtcaaa agtatttaac cgagtaatca aatgatcaaa
    25261 tgaagttgga taaagcgaaa atgaattcaa ataaaaaggc tctaagtcaa atcgtgttgt
    25321 tttcattaaa tcactgaaag gaaatgcttc tgcgtttcct tttttctttt tgttgtgcgc
    25381 tgtatacctt gaggatattc actggtggcg tacacttaaa tagtaatata ttgtgttact
    25441 ttatgtctgt ggtaatagtt atagttgctt accaatgtat gttcattttt cgaaatgttg
    25501 acttcataat ccttggctta atgtttgaat ttttttggtg atagattttt tcagccgtat
    25561 aaaataaatc gtaattctat tatagagagt tgtgattata aaaagatagc aagaaaaaaa
    25621 aagaaagttt ctatacattt taaatatttt attcctattt ctattgctaa aaaagtagaa
    25681 attctatatt tacctttaac tctcgcttct cttgcaaatg tagttagcat tttaatcgat
    25741 aataaactca ggtagttata ttaccttgaa atgattgata attttgtcac ttctattttt
    25801 tttgcaat
//
LOCUS       DARBET010000034        24575 bp    DNA     linear   BCT 07-NOV-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4027
            SAMEA114569841-rid20112183.denovo.034, whole genome shotgun
            sequence.
ACCESSION   DARBET010000034 DARBET010000000
VERSION     DARBET010000034.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA114569841
            Sequence Read Archive: ERR12203666
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 24575)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 24575)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001972618.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001972618.1
            Genome Coverage       :: 132x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/07/2023 13:18:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,041
            CDSs (total)                      :: 2,975
            Genes (coding)                    :: 2,908
            CDSs (with protein)               :: 2,908
            Genes (RNA)                       :: 66
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 67
            CDSs (without protein)            :: 67
            Pseudo Genes (ambiguous residues) :: 0 of 67
            Pseudo Genes (frameshifted)       :: 26 of 67
            Pseudo Genes (incomplete)         :: 28 of 67
            Pseudo Genes (internal stop)      :: 20 of 67
            Pseudo Genes (multiple problems)  :: 6 of 67
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..24575
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA114569841-rid20112183.denovo.034"
                     /isolate="VRE4027"
                     /db_xref="taxon:1352"
                     /country="Denmark"
     gene            complement(152..550)
                     /locus_tag="SCJ86_001561"
     CDS             complement(152..550)
                     /locus_tag="SCJ86_001561"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295200.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="P-II family nitrogen regulator"
                     /protein_id="HEG1169102.1"
                     /translation="MKDLVALNLHMIVTIVDGGMGEDVIDFSKEAGAAGGTILHGRGS
                     GVHDSGKFLGLDISPEKDIVLTLVPDSLTNKVMDAIGQGINIDIPGNGICFSIDVDKV
                     IGITKIHQYREIIGMQELLDENNDREKSES"
     gene            complement(547..1320)
                     /locus_tag="SCJ86_001562"
     CDS             complement(547..1320)
                     /locus_tag="SCJ86_001562"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331418.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1538 domain-containing protein"
                     /protein_id="HEG1169103.1"
                     /translation="MSFWQQVIDGIEIVTMEVILAVAPIVILFVLMNIISFRMRKKRF
                     FDIMKGFLITTIGLILFLHGVNMAYVPVGQHLGSAIAGLPYNYILVPLGFLMGFLVGF
                     AEPAIHVMIKQVEELSEGRIRSKIMLMVISIGIGAAVGLSMWRLLVGFSLYYFLIPGY
                     GLAFILGRKVDKIFLAMAFDNGGVATGPMCSTFILSMSVAIAAQIEGRDPIIDGFGVV
                     AMIALTPILSTLVLGYIYKRKDEKDRIEKERLQKLGESK"
     gene            complement(1317..2000)
                     /locus_tag="SCJ86_001563"
     CDS             complement(1317..2000)
                     /locus_tag="SCJ86_001563"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005879910.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1538 domain-containing protein"
                     /protein_id="HEG1169104.1"
                     /translation="MLKNFKEVIAAILPMTVLIVILTFVFAPLEGEDLASFLVGAAIM
                     MIGMTLFLFGADYSMMEVGDLVGKYMIKKKSLTVLVSLGFAIGIVITIAEPSVQVLGQ
                     QVYDISDGEIGRVLLIGIVSVGTGVFLAFALLRVVFKLSYYQLMAIGYIGVLIASFFT
                     SSEFMPIAFDSGGVTTGPVTVPFILALAGGMTSMIKQKKNENDSFGMVGIASLGPILA
                     VMILGVIFQ"
     gene            complement(2167..3054)
                     /locus_tag="SCJ86_001564"
     CDS             complement(2167..3054)
                     /locus_tag="SCJ86_001564"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002351179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HEG1169105.1"
                     /translation="MKNMVEKETIERPNVKKQALELRSTIGSVLKYTALSILSFISIF
                     PFVWMVLGMTNTPVDITSGKIKIGSNLIINFQNLFSNELNFTRSLWNSAMIALVTTVF
                     ALLISSMAGYGFEIYQSKRKDLIFNILLLSMMVPFAALMIPLYRMFSQLNGTVLGINS
                     FLVVILPSVSTAFLIFFFRQNVKSFPKDLVEAARLDGLKELSIFFRVYMPTAKNTYAA
                     AAIITFMSSWNNYLWPLVALQSPDKRTVPLVLSAMGASYTPDYGMIMAGLVIATLPTA
                     IVFFVLQKQFVQGMIGSVK"
     gene            complement(3051..3920)
                     /locus_tag="SCJ86_001565"
     CDS             complement(3051..3920)
                     /locus_tag="SCJ86_001565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628995.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HEG1169106.1"
                     /translation="MKQKDLFKNGHAFLILPVILISIMVFIPMVLALITSFQSGPPVN
                     MTFNGLGNYQRMLTDATFKKAFFNTFLYLLVQVPIMLFLALFISNLLNDKKLKYKGLF
                     RTAIFLPCITSMVSYSLIMKSLFSQSGLVNNLLLGIGMIDTPIGWLTHPIWARVLIIL
                     SITWRWTGYNMIFFLSGMQNIDSEIYEAASIDGASKWQQFIKVTIPNLKPIILFTSIT
                     STIGTLQLFDEVQNITGGGPANATTTLSQYIYNLSYKFTPNFGYAAAVSFVIVSFIVI
                     LSIIQTKVGGESR"
     gene            complement(4057..5343)
                     /locus_tag="SCJ86_001566"
     CDS             complement(4057..5343)
                     /locus_tag="SCJ86_001566"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374522.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter substrate-binding
                     protein"
                     /protein_id="HEG1169107.1"
                     /translation="MNIRKLIAGVVCLAAASGFLTGCGSASSTADKEEKVTVWAWDET
                     FNIKAVNEAKKVYENEETEIEVVTMSQDDIVQKLNTALASGNTEGLPNIVLIEDYRIQ
                     GYLTSYPDAFANLSAIVKEDHFAPYKFAVNKVGDKIYGVPFDSGVTANFYRTDLMAEA
                     GYTEEDMENLKWDEYIQVARDVKEKTGKKIAEVNPSDLGRVRMIMQQAGEWYTSEDGE
                     TVTIQDNASLKYGLELFATLLKEDLVEQTSDWNAGVTAIQSGAVASSPTGAWYSSTIQ
                     GAENQSGKWKIAPIPALPENLQKAQASNLGGAGWYVIKGVAGEDSAKDFLEATFASNE
                     ELMGTLAKEIGLVSTMLSANEQEAYQEASEFYSGQKIFDDFSTWTSEIPEVNYGHETY
                     AIEAVVAEALHRIINGEETDKVLADTQKQVEAQLAN"
     gene            5616..6194
                     /locus_tag="SCJ86_001567"
     CDS             5616..6194
                     /locus_tag="SCJ86_001567"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289134.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HEG1169108.1"
                     /translation="MPIRVILIDDEQTILDGMKQLINWSEHGFQLIGTFSSPMKALDF
                     SLVHAIDIVVTDVMMPELNGIELSRLLKQQQPETQILILSSYDEFDMVKDSFKEGVAD
                     YLLKPRLSPDFFLNSLTAVSQKIKKKPNKSPITNRYEQICENLSQQIAGQTRPLLDPL
                     LFEHTLFFCYIPKNGHSVQNKALNKNSRSPIH"
     gene            6194..7123
                     /locus_tag="SCJ86_001568"
     CDS             6194..7123
                     /locus_tag="SCJ86_001568"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346348.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HEG1169109.1"
                     /translation="MDSDDSFTIYPVSTFSEESGYVINTDSKDTLRKFVQKFRQEVFP
                     GDFFVVSDSFAIQDINEIFQEIRNCTKGQRFYHKGKNLAYQHELLPLLTNHEQQASSY
                     LTKIVHKDYALAVKELENFFTTILEQPIDPAYLKHEAINRFYALLNALADEHVGNEEL
                     SLLKIALPSLLADAEYFEDFSILVKETLEKLTHFSANAKKDNFDILTLIYEFVQENYE
                     KEINLQLLSEKYHFSYSYLSAIFTEKFGINFSKYLKKVRISKAKLFLTETRLTLTEIC
                     EKVGYTELGYFSRVFKEETGMTPSQYRKGTFVK"
     gene            7120..8856
                     /locus_tag="SCJ86_001569"
     CDS             7120..8856
                     /locus_tag="SCJ86_001569"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324964.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine kinase"
                     /protein_id="HEG1169110.1"
                     /translation="MRKKIKEHELFAKLLFILFIGLFLQAIVISLFIYHRSRDAYIQL
                     FNQSNDVVLKKIQSEFESLNDTIENTLAAFDSNPAVKSYFSNDPAQHMEQLQQLRTIQ
                     KMNDSLSKIHPMIDYDVLIFGENGRTFVGNDMLTAVSADSFFQSAIAQRVNERAADTQ
                     MLFAHHGLTLRDKKAPSVFFVKKLKNTLNHVYGYAVLSISSKQLANLFQSVVNPEISK
                     ISFMNEEQTIIASNEENTIGSRSMIFKKLTVGETTISNGQQLTRLPLYRQDCTLLSQV
                     DLHSLSNQMGVIFPIILYNIVTLAFAGSLVFMYLDRHTKSIYRLIDSLKRLEEDGLKA
                     TVPVQGSHEVQLLGNTINQLLSNAQHHYEQTIQDEKKKRSLEIQTMQAQIQPHFIYNT
                     LTSLKFLIWQKKTDEAVQGIDSFITLLRSTIGKKEEVIPVKEEIKSVQSYIDLLSMRY
                     GERISAKIMIPDELQSLSMPNMILQPIIENAYLHAFHNKTQGFITVYGKTKNQLLLFE
                     VIDNGDGFDTENTKQKNDHFSGIGISHVDERIRLMYGDAYGLTIQSVIGTGTTVTICL
                     PLLPKNQSEYQK"
     gene            complement(8919..9755)
                     /gene="pheA"
                     /locus_tag="SCJ86_001570"
     CDS             complement(8919..9755)
                     /gene="pheA"
                     /locus_tag="SCJ86_001570"
                     /EC_number="4.2.1.51"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338342.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prephenate dehydratase"
                     /protein_id="HEG1169111.1"
                     /translation="MNVSYLGPESSFTYQAACQLFPTEQLTAYASIPVCLRALFRKQV
                     DLAVVPVENSLEGAVHHTIDLLSKHPEVEVKSEIVLPIKQQLLGNPATKITKILSHPQ
                     ALAQSQQFLETHYPNVPLVATESTTAAAMYVAEHPKEDAAAIASLETAQHVGLEILAE
                     NIQDNELNQTRFWIVGDRKMTSQQSAPVKMSVILTLPANRPGMLHKMLAAFGWREINL
                     SKIESRPLKTSLGEYFFVIDLLLDRPMTLVENALQEIKMLGGESQILGCYPVLTVEET
                     RR"
     gene            complement(9752..10261)
                     /locus_tag="SCJ86_001571"
     CDS             complement(9752..10261)
                     /locus_tag="SCJ86_001571"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="shikimate kinase"
                     /protein_id="HEG1169112.1"
                     /translation="MASILLIGFMGAGKTTIGKGLAQCLQKEYVDLDTKIEEHIQLSI
                     AEYFHYYGEKSFRKVESDILRKLSNEDKIIATGGGIVQSAENRRFLKTQPIVLYLEAE
                     ADCLVDRIQQDETSIRPLALGKTREEIKALLAQRLSWYEESATHRINTTNQTPEKIID
                     TIIERIKSI"
     gene            complement(10254..11549)
                     /gene="aroA"
                     /locus_tag="SCJ86_001572"
     CDS             complement(10254..11549)
                     /gene="aroA"
                     /locus_tag="SCJ86_001572"
                     /EC_number="2.5.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338785.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-phosphoshikimate 1-carboxyvinyltransferase"
                     /protein_id="HEG1169113.1"
                     /translation="MQLLQQIHGLRGTVRIPADKSISHRSIMFGAIAEGTTTIQNFLR
                     AEDCLSTLHAFQQLGVEIEEEEEVIKIHGRGSHSFVQPTESIDMGNSGTTIRLLMGIL
                     AGQPFTTTLFGDASLSKRPMGRVMEPLREMGADLQGEENDQYLPITVTGTRSLSPIRY
                     NMPVASAQVKSALLFAALQAEGTSVIVEKERSRNHTEEMIRQFGGRITVEDKTIIVTG
                     PQKLTGQQITVPGDISSAAFFLAAGLLVPESQLLLKNVGVNPTRTGILDVLEEMGAAI
                     TQTNHNEQNQSADLSVKTSHLKKATIGGEIIPRLIDELPILALVATQAEGITIIKDAE
                     ELKVKETNRIDAVAEELQKMGAKIKATADGLIIHGPTPLHGAKVSSRGDHRIGMMLQV
                     AALIADGPCELEGAEAVSISYPAFFDDLAELVSGGEAHG"
     gene            complement(11562..12653)
                     /locus_tag="SCJ86_001573"
     CDS             complement(11562..12653)
                     /locus_tag="SCJ86_001573"
                     /EC_number="1.3.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345155.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prephenate dehydrogenase"
                     /protein_id="HEG1169114.1"
                     /translation="MAQHVFIVGLGLIGASMALCIRQTNPELIVVGWDSQSKTREQAV
                     EQQIVDYVASDFETGAEQADVILLAVPVRTTLAYLDVLERINLSDHVIITDVSSTKQQ
                     VVTSAEQKNLRFVGGHPMAGSHKSGIQAADAKLFENAYYIFTPLEKTKKDVEKLQELF
                     CGTKAKYVTLTAREHDRITGMLSHFPHILAAGLVNQAEQFNQEYPRAKQLAAGGFRDI
                     TRIASSDPVMWTDILLSNKQILLERLADWQQEMTQIAEWIMTENQSEIFSFFNRAKES
                     RDQLPVHKQGAIPAFFDLFIDVPDEPGVIAEVTGLIGKAGVSLINLKIQETREDILGI
                     LQISFKNQQDLLQAKRIIMSNTSYHCWIK"
     gene            complement(12670..13836)
                     /gene="aroC"
                     /locus_tag="SCJ86_001574"
     CDS             complement(12670..13836)
                     /gene="aroC"
                     /locus_tag="SCJ86_001574"
                     /EC_number="4.2.3.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001269368.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chorismate synthase"
                     /protein_id="HEG1169115.1"
                     /translation="MRYMTAGESHGPELTAIIEGLPAGLPLSEEEINHELARRQAGYG
                     RGGRMKIEKDRVRITSGIRHGKTIGSPVTLVVENKDWKNWTTVMSVAEVPEKDQSLRR
                     VARPRPGHADLVGGMKYHHRDLRNVLERSSARETTMRVAIGAIAKKILEELDIIVAGH
                     VICLGGIQAEIPGNISIQEIKEISEASEVRVVDPAVEQRIKDLIDQTKKNGDTIGGIV
                     EVLVGGVPAGLGSYVQWDTKLDAKIAQAVVSINAFKGVEFGVGFTSGSLPGSEVMDEI
                     LWEEERGYFRRTNNLGGFEGGMTNGEPLVIRGVMKPIPTLYKPLQSVDIDTKEPYKAS
                     VERSDSTAVPAASVVAENVVATVVATELLSKFPSDSLVELKAAVKEYRDYLKHY"
     gene            complement(13838..14902)
                     /gene="aroB"
                     /locus_tag="SCJ86_001575"
     CDS             complement(13838..14902)
                     /gene="aroB"
                     /locus_tag="SCJ86_001575"
                     /EC_number="4.2.3.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345157.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-dehydroquinate synthase"
                     /protein_id="HEG1169116.1"
                     /translation="MLTVHVDNQKYDIHIEKGALEQAGSWLQTIWRPQKVAVITDTNV
                     APLYAEKIMFQLRQANFEPVLSIVPAGETSKSLEQAAELYDFLADNDFTRSDGILALG
                     GGMVGDLAAFVASTFMRGIHFVQIPTTLLAQVDSSIGGKTAVNTNKAKNLVGTFSQPA
                     GVLIDPEVLKTLPVRRLREGIAEIIKSAAIADATLWKQLNELKDEKDLLEHAVPIITA
                     TLEIKRKVVEEDVFDQGNRLILNFGHTIGHAIENTAGYGVVSHGEGVAIGMVAITSHA
                     EKIGLSPTGSTAALERILQKFHLPITYDLSHPDELFHAITHDKKARGSQLNIILLEKI
                     GQAKIVPVPTETFKDYLGGL"
     gene            complement(14935..15957)
                     /gene="aroF"
                     /locus_tag="SCJ86_001576"
     CDS             complement(14935..15957)
                     /gene="aroF"
                     /locus_tag="SCJ86_001576"
                     /EC_number="2.5.1.54"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338346.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-deoxy-7-phosphoheptulonate synthase"
                     /protein_id="HEG1169117.1"
                     /translation="MIVIMKKEATEEQIKKVVDHIEKEGFKVSIDQGTERVVIGLKGD
                     TRSLQEGAFTRYEGVENAVRILNTYKLTSREFHPGNTVVDVDGVKIGDGSFVTMAGPC
                     SIEGLDQIRECARMAKAGGAKILRGGAFKPRTSPYAFQGLEEEGLKYIRQAADEFGMK
                     VITEVMDEGHIDMVAEYSDILQIGARNMQNFKLLSAVGKTGKPVGLKRGISGTINEWL
                     NAAEYIAVEDKSPVIFIERGIRTYETATRNTFDLSAVPLMKKLTHFPVIVDPSHGTGI
                     WELVPPMARAGVASGADGMIVEIHPDPANAWSDGPQSLNEKTYSRMMKEVAIMKEAME
                     KIQTLD"
     gene            complement(16616..17590)
                     /locus_tag="SCJ86_001577"
     CDS             complement(16616..17590)
                     /locus_tag="SCJ86_001577"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329288.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-galactosidase small subunit"
                     /protein_id="HEG1169118.1"
                     /translation="MKNTVNKSHMDTEKVAIVFGDCTLGVKSGNTHYIFSYTRGGLES
                     LNKNGKEWLYRETLPVFWRATTDNDRGSGFGFKSGMWLGAGLYPKVKEIEVLVDNKKI
                     DLPIAPVNNQYSSNEYAETVEIIFTLEINTVPKTQVTISYQIAGEGKMLVNMHYYGQK
                     GLPELPALGLRFIMPTKAKFFEYEGLSGETYPDRMAGGVEGIYKVAGLPVSPYLVPQE
                     CGMHMETKWVELTRNQTLDNTDKDQEDFSLRFEQQDTPFAFSALPFKAEELEQATHIE
                     ELPPERRTVLTIFAKVRGVGGINSWGADVESPYRISSEEDHEIGFIIQ"
     gene            complement(17571..19454)
                     /locus_tag="SCJ86_001578"
     CDS             complement(17571..19454)
                     /locus_tag="SCJ86_001578"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343218.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 2"
                     /protein_id="HEG1169119.1"
                     /translation="MKANIKWLDDPQVFRVNQSNAHSDHVCYATREEVETTSSLKQSL
                     NGSWRFCYSPNPSVRPKTFYKKDFDACSFDEITVPSHIELANYDKIHYINTMYPWEGH
                     VFRRPPFTTSPEKEAEGIFSAAKDNPVGSYLTTFRLEEGLKGKQISIHFAGVEQAMFI
                     WLNGHFIGYAEDSFTPSEFDLTPYLEEENILAVEVYKRSTAAFLEDQDFFRFFGIFRD
                     VFLVARGQNHLEDLWIKPRLDDKCQNGKLEVDLSFEQVAKNSQLNVAILDPDGKEIYQ
                     QQYPLKEQMTIRIDGFENVALWGHFQPNLYKLELETIDEEKRVTEYIDYSFGFRKIEI
                     KDKIIYFNGKRMIINGVNRHEWHPKKGRAITIEDMEKDLAIIKNAKINGVRTSHYPNQ
                     ITWYYLCDQAGIHVMAETNLESHGSWQKMGAFEPSYNVPGAVPQWQAAVLDRAKTNFE
                     TLKNHPSILFWSLGNESYAEENIRQMNDYFKKVDPDRLVHYEGNFPNPAFEDSISDVK
                     SCMYAPPEEIIDYLENKAEKPFILCEYMHDMGNSLGGMDSYMTLLDRYEMYQGGYIWD
                     FKDQAIQVTDPITGREVMRYGGDFDDKPSDYEFSGNGIVFADGQEKPAMQEVRYYYEK
                     YSK"
     gene            complement(19472..21697)
                     /locus_tag="SCJ86_001579"
     CDS             complement(19472..21697)
                     /locus_tag="SCJ86_001579"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292032.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-galactosidase"
                     /protein_id="HEG1169120.1"
                     /translation="MDITHRKTDKNFILFDEKSRVFHLRNTFLSYLIKIEESNVLAHI
                     YFGKPVKQYKDNKNYPRRDRGFSGNVPLNPDRSLSKDTLPQEYSSHGSMDFRTPASII
                     QRKNGSDLLDLRYDSHYITDGKPEIEGLPQTYVMDKSEAQTLVISLKDRETAIYFDLF
                     YTIFTDRAVITRSVKIRNETGETIKLEKAASFQLDFAHTRRFDEVIALPGAHVNERQI
                     SRQSVLSGTKVFESRRGTSSHHMNNFIALVHHHTTENTGEAIGLQFVYSGNHSFELEK
                     DQINQLRVVGGINSHRFSWELNAGQSFQTPEMILSYSSQGLNKMSQIHHELLRERIAR
                     GRHQFAERPILVNNWEATYFDFNSEKIKAIIDEAKELGIEMFVLDDGWFGKRDADNSS
                     LGDWFEYEGKLTNGLREIADYAHSKGLKFGLWFEPEMISVDSELYRTHPDFLMQEPGR
                     MPSASRSQHVLDFTRLDVRQTIEKQMRKILDTIPLDYIKWDMNRSLSDVYSITLDPQR
                     QGEVAHRYMLGLYELLEHLTTDYPEILWEGCSGGGGRFDAGFIYYMPQSWTSDNTDAV
                     ERMKIQYGTSLAYPISSITAHVSAVPNHQTDRSTSLKTRGETAMSAVFGYELDLTKLS
                     PEEKKQVKDQIISYQTIRPVIQYGHYYRLASPFEENIAAWMFVSPKQDEAIVFLGRIL
                     ASAQPAFHEVYLMGLDDEALYQEQTSKRIFSGAELMTVGLYFPDFQGDFQTELLHFKK
                     L"
     gene            21812..22846
                     /locus_tag="SCJ86_001580"
     CDS             21812..22846
                     /locus_tag="SCJ86_001580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HEG1169121.1"
                     /translation="MVGIRDVARKANVSPATVSRVLNNDQTIHVAQTTRQRIVEAAAA
                     LDYDISTRKYIKKRMPSIGVISTIGKASEEQDIYYKHLRVGMEEEARRLHLGMNRIYN
                     LSDNPKQWEDLDQLGAVIVVGTVTKESITALLKQNMQIVVVDNPDIQQEVDMVYADLE
                     RMTKTVLELFLENGHKRIAYIGGYKVDMDEFGGKQISINEKRMRAYQHFMIDHHLEKY
                     MSYHLGGWTEKQGEVLATNLLENESPLPTAILVGSDPMAIGVYRALKQKNIQIGEEIV
                     VASFDNIETIANLSPGLTSVQINAKSIGQAAVRMALERIDGIRDEPIILTYPARLIVR
                     ESFIPRTKRY"
     gene            complement(22913..23926)
                     /locus_tag="SCJ86_001581"
     CDS             complement(22913..23926)
                     /locus_tag="SCJ86_001581"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341802.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HEG1169122.1"
                     /translation="MKKKILKFIGWFFGIILGIAFAIFLAFQLSPRPGAFLINHMFSN
                     TVQITDKKTFDQAEKNVAKKTDLTYESKYKENTYDIYYPKNSKGPVPVLFWVHGGGFV
                     GGDKSGVKEFATKLVSDANIAVVAMNYELAPDSEYPNQVLQVNELIKDLLAEEKDDKL
                     LDMEQLFFGGDSAGSQIALQYAAIQTNDAYAKQMKIKQLLPKDSLKGTISYWGPVDLK
                     LTAQQHSDDRFMKFFVKTVAWSLIGTKDWKTSEQLKEASVADHVSKEFPPTYITDGNA
                     YSFQEQGQALENRLTELKVPVQSLFYTDTKKEITHEYQFDYTLKESQNCYQQTLDFVN
                     KYK"
     gene            complement(24004..24192)
                     /locus_tag="SCJ86_001582"
     CDS             complement(24004..24192)
                     /locus_tag="SCJ86_001582"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methionine synthase"
                     /protein_id="HEG1169123.1"
                     /translation="MEEHQQVIDRIYEATRYLPLDRLCLSPQCGFASTEEENVLFEEQ
                     WKKFLLSKALQKKFGENH"
BASE COUNT         7061 a         5341 c         4397 g         7776 t
ORIGIN      
        1 gcatattttg aaaagaaagt ctgatgctta gagtagaatt tcataaaaat ttactatttc
       61 ataagtattc acgagaaaaa tgcaaacaaa aaagcggaaa taacgctcat ccgccttctt
      121 gtttgtgttt atttcctctt caaacgcttg tctatgattc agacttttct ctatcgttat
      181 tttcatctaa aagttcttgc attccgataa tttctcgata ttgatgaatc ttagtgatac
      241 caatgacttt gtctacatca atactaaaac aaataccatt acctggaata tcgatgttga
      301 tgccttgacc aatcgcatcc attactttat tcgtcaaact atcgggtact aatgtcaaca
      361 caatatcttt ttctggcgaa atgtccaaac ctaaaaattt ccctgaatca tgtacaccag
      421 aacctcttcc atgtaagatc gttccgcctg ctgcacctgc ttcttttgaa aagtcgataa
      481 catcttcgcc cattcctccg tcaacgatcg tcacaatcat gtgaagattc aatgctacaa
      541 gatctttcat ttgctttccc ccaatttttg caatctttct ttttcgattc tgtctttttc
      601 atcttttcgt ttataaatat atcctagtac taaagtggat aatattggtg tcaatgcaat
      661 cattgcaacc acaccaaacc cgtcaatgat cggatctctt ccttcaattt gtgcagcgat
      721 tgccacgctc attgataaaa taaatgtcga acacatcggg cccgttgcta ctccaccgtt
      781 atcaaaagcc attgctaaaa agatcttgtc cacttttcgt cctaaaataa atgctagccc
      841 gtatcctggg atcaagaaat aataaagaga gaatccgacg agcaaacgcc acatagacaa
      901 tcctactgct gcgccgatcc caattgaaat taccatcagc atgattttcg atcggattct
      961 cccctcgctc aattcttcaa cctgtttgat cattacatga atcgccggct cagcaaaccc
     1021 tactaagaac cccattaaaa agccaagggg aaccagtata taattgtaag gcaatcccgc
     1081 aatagcactg cctaaatgct gtcctaccgg tacataagcc atattgactc cgtgcaggaa
     1141 aaggatcaac ccaatagtcg ttatcaagaa acctttcata atgtcaaaga aacgcttttt
     1201 gcgcatacga aaactaatga tattcattaa aacaaatagg attacgatag gtgccactgc
     1261 caaaatgact tccatggtaa cgatttcgat tccatctatc acttgctgcc agaaactcat
     1321 tggaagatca cccccaaaat catcacggca agaatcggcc ctaacgaagc aataccgacc
     1381 attccaaaac tgtcattttc atttttcttt tgtttgatca tactagtcat ccctccggca
     1441 agggccaaga taaatggaac agttactgga ccagtagtca ctccccccga atcaaaagca
     1501 atcggcataa attcggaact ggtgaaaaat gaggcgatca aaacgcctat gtagcctata
     1561 gccatcaatt gataatagga tagcttgaat acgacccgaa gcagcgcaaa ggctagaaat
     1621 actccagtgc cgacgctgac gatcccaatc agcaacactc tgccaatctc tccgtcagat
     1681 atatcatata cttgttgtcc caaaacttgc acggaaggtt cagcaatcgt gatcacgatc
     1741 ccgatagcaa atcccaaact gaccaaaacg gtcaaacttt tctttttgat catatatttc
     1801 cctactagat ctccgacttc catcatagaa taatctgctc caaacaaaaa gagcgtcatt
     1861 ccgatcatca tgattgccgc accaaccaga aatgaagcga ggtcttctcc ttctaacggt
     1921 gcaaaaacaa acgttaaaat gacaatcagg acagtcatcg gcaatatagc agcaatcact
     1981 tctttaaaat tttttagcat gtatcctcct cctttactct ttaagtttac cttaaaatga
     2041 aagagttttc gagcacaaac ttaaaacgtt atcatttaat ttttctcaac ataaaataac
     2101 acacgtccca aaagtatctt tttacttgga aaaatgactt tgaaggaacg cgcttattgt
     2161 gtttttttat tttacagagc cgatcattcc ttgaacgaat tgcttttgca aaacaaaaaa
     2221 tacgatggca gttggtaaag tagcgatgac taatccagcc atgatcattc catagtctgg
     2281 tgtataggaa gcacccatag cagataaaac aagcggaacc gtccgtttat caggagattg
     2341 caaagctact aagggccata aataattgtt ccagctactc atgaatgtga tgattgcagc
     2401 cgcggcatag gtatttttag ccgtcggcat atacactcta aaaaaaatac ttagttcttt
     2461 tagaccgtct aatcgagcgg cttctactag atcttttgga aaagatttaa cattttgtcg
     2521 aaagaagaag atcaaaaaag cagtgctaac agatggcagg atgacaacca agaaactgtt
     2581 gattcccaaa accgttccgt tcaattgtga aaacattcga tataaaggga tcatcaatgc
     2641 cgcaaaagga accatcatcg atagcagtag aatattgaaa attaggtctt ttcgtttact
     2701 ttgataaatc tcaaatccat atcctgccat tgacgaaatc agcaatgcaa acacagtggt
     2761 caccaaagcg atcatcgctg aattccaaag ggaacgagta aaattcagct cattggaaaa
     2821 caaattttga aaattgatga tcaagttact gccaatcttt atttttcccg atgtgatatc
     2881 cacgggtgta ttcgtcattc ccaaaaccat ccagacaaaa gggaaaatgg aaataaatga
     2941 taggatgctt aatgctgtat actttaatac tgacccaatt gtcgaacgaa gttctaatgc
     3001 ttgttttttg acgttggggc gttcgattgt ttctttttct accatatttt tcatcgactt
     3061 tctcctccta ctttcgtttg gattatcgac aatatcacaa taaaggaaac aataacaaag
     3121 gaaactgctg ccgcatatcc aaaattaggc gtgaatttat aactcaaatt atagatgtat
     3181 tgagataggg tggtcgttgc atttgctggt cccccgccag taatattttg tacttcatca
     3241 aaaagctgca atgtcccgat cgtcgaagta atggaagtaa acaaaatgat tggttttaaa
     3301 tttgggatcg tcactttgat aaactgctgc catttagaag caccgtcaat agatgctgct
     3361 tcataaatct ccgaatcaat attttgcatg cctgaaagga aaaaaatcat attgtatcca
     3421 gtccaacgcc aagtgatcga aagaatgatc agaactctcg cccaaattgg atgagtcaac
     3481 cagccaatcg gtgtgtcaat cataccaatg cccaacaaca agttgtttac aaggccagat
     3541 tgagaaaata gacttttcat gatcagagaa taactgacca tcgaagtgat acatggaaga
     3601 aagatagcgg ttcgaaacaa ccctttatat ttcaactttt tatcatttaa aaggttggaa
     3661 ataaaaagtg ctagaaacaa catgatcggt acttgtacaa gtaaatacag gaatgtatta
     3721 aaaaaagctt ttttaaaagt tgcatctgtc aacatccgct gataattacc taacccgtta
     3781 aatgtcatat tgacaggggg accactttga aaagacgtga tcaaagccaa aaccatcgga
     3841 ataaaaacca tgattgatat caaaatgact ggcaagatta aaaaagcatg cccgtttttg
     3901 aataaatctt tctgcttcat tcttttccct ccaattctat cattgattat ctacatgaaa
     3961 agacaagaaa gacaatatct gtaaggcaga cgtgatcttt tttgtctttt cgtttgtctg
     4021 atgaaaaagc gaagaaattc acttttttac caccgatcaa ttagccaatt gtgcttctac
     4081 ttgtttttgt gtatctgcta gtaccttgtc tgtctcttct ccattgatga ttcgatgaag
     4141 agcttcagct acgactgctt caattgcata ggtctcatgt ccgtaattca cttctggtat
     4201 ttcagatgtc catgttgaaa aatcgtcaaa gatcttttga ccactgtaaa attcagaagc
     4261 ttcttgataa gcttcttgtt cattcgcgct aagcatcgta gatactagtc cgatttcttt
     4321 cgcaagagtg cccatcagtt cttcattcga agcaaacgtt gcttctagga aatctttcgc
     4381 tgaatcttct ccagctactc ctttgatcac ataccagcca gcgccgccaa ggttagaagc
     4441 ttgcgctttt tgcagatttt ctggaagtgc aggtattggt gcaatcttcc attttccaga
     4501 ttggttttct gctccttgga tcgttgaaga ataccaagcg ccagttgggc tagaagctac
     4561 tgctcctgat tgtatcgcag ttactccagc attccagtca gaggtttgtt cgactagatc
     4621 ttctttcaaa agagtggcaa acaattcaag accatacttt aaagatgcat tgtcttggat
     4681 cgtgactgtc tccccatctt cagatgtata ccattctcct gcctgttgca taatcatcct
     4741 tactcgacca aggtcagaag gattgacttc agcgattttc tttcccgtct tttctttgac
     4801 atctcgcgct acttgaatat actcgtccca cttcagattc tccatatcct cttctgtata
     4861 acctgcttct gccatcagat ccgtacgata gaagtttgct gttacgcccg aatcgaaagg
     4921 aacaccgtat attttatctc ctactttgtt taccgcaaat ttatacggtg caaaatgatc
     4981 ttcctttaca atagcagaca aattagcgaa tgcatctgga taagaagtca aatagccttg
     5041 tatcctataa tcttcaatca agacgatatt gggcaatcct tctgtattcc cgctcgctaa
     5101 agcggtgttc agtttctgaa cgatatcatc ctgagacatc gtaacaactt ctatctctgt
     5161 ttcttcgttc tcataaactt tttttgcttc attcactgct ttgatattga atgtctcatc
     5221 ccacgcccag acagtgactt tttcttcttt gtctgctgta ctactagcac ttccgcatcc
     5281 tgttaagaaa cctgatgctg ccgctaaaca aacgactcca gcaattaact ttctgatgtt
     5341 catttttatc cgctcctttt ccatccatta gttgtttacg tttgctacat tcatcatacc
     5401 aaaatttgaa aacggtttct ttattattca gatacttcaa aaatttctcc taaaataata
     5461 aaaaaataca aatcaactat gacaaaagga ataatcttag catctagacc aaagatcgaa
     5521 atggtgaggt ttctttaaaa taaaaatatc cttgcatttt ctcctagaaa taagtatttt
     5581 taaattacaa gactattctt aaggcgggta aaaaaatgcc aatacgcgta attcttatcg
     5641 atgatgaaca gacgatttta gatggaatga aacagttgat caattggtct gagcacgggt
     5701 ttcaattaat agggactttt tccagtccga tgaaagcgct tgatttttct ttggtacatg
     5761 caatcgatat cgtggtcaca gatgtaatga tgcctgaatt aaatggaatc gaattatcgc
     5821 ggctattaaa acagcaacaa cctgaaacac agatccttat cctctctagc tatgatgaat
     5881 ttgatatggt taaagattct tttaaagaag gcgtcgctga ttatctttta aagcctcgat
     5941 tgagtcctga tttttttcta aattccttaa ctgccgtttc acaaaaaatc aaaaaaaagc
     6001 ccaacaaaag cccaatcacc aatcgttatg agcagatctg cgagaatctt tcacagcaga
     6061 ttgctggaca aactcgtcca ttgcttgacc ctcttctttt tgaacacacc ttgtttttct
     6121 gttatatacc gaaaaacggc cattcagttc aaaacaaggc tctaaacaaa aacagtcgat
     6181 cacccattca ttgatggata gcgacgattc gtttactatt tatcctgttt ctaccttttc
     6241 ggaagaatcc ggctatgtaa tcaatactga ttccaaagat acacttagga agtttgttca
     6301 aaagtttcgt caagaagttt ttccaggtga tttttttgtt gtttctgatt cttttgcaat
     6361 ccaagatatc aatgaaatat tccaagagat cagaaattgc accaaaggac aacgtttcta
     6421 tcataaggga aagaaccttg cttatcagca tgaacttctt ccgttattaa ccaaccacga
     6481 gcagcaagca agtagttatt taacaaaaat cgttcataaa gactacgctt tagctgtcaa
     6541 agaacttgag aatttcttca cgacgatatt ggaacagcct attgatccag cttatttgaa
     6601 acatgaggca atcaatcgtt tctatgcatt actcaacgct ttggctgacg agcatgttgg
     6661 gaatgaagaa ctttctttgc tcaagatcgc ccttccttct ttattagccg atgctgagta
     6721 tttcgaagat ttttctattc ttgtcaaaga aacacttgag aaactcaccc acttttctgc
     6781 aaatgcaaaa aaagacaatt tcgatatctt gactttgatc tacgagttcg tccaggaaaa
     6841 ttatgagaaa gaaatcaatt tacaactttt atccgaaaaa tatcattttt catatagcta
     6901 tctctcagcc atctttactg aaaaatttgg tatcaacttt tcaaaatatc taaaaaaagt
     6961 ccgtatcagt aaagccaagc tctttttgac ggaaacacgg cttactttga cggaaatctg
     7021 tgagaaagtc ggctataccg aactgggtta tttctccaga gtttttaaag aagagacagg
     7081 aatgacacct tctcaatacc gtaaaggaac ttttgtaaaa tgagaaaaaa aatcaaagag
     7141 catgaactct ttgccaaact tctgttcatt ttgttcatcg gtctttttct gcaagccatc
     7201 gtcatttctt tgtttattta tcatcgatca agggatgcct atatccagct gttcaatcaa
     7261 tcgaatgatg tggtactaaa aaagattcaa tctgaatttg aaagtttgaa tgatacaatc
     7321 gagaatacat tagccgcttt tgattctaat ccagctgtca aaagctattt ttccaatgat
     7381 ccagcccaac atatggaaca gcttcagcaa ctaagaacaa tccaaaaaat gaatgactct
     7441 ctttcaaaaa tccatccaat gatcgattac gatgtattga tctttggaga aaatggaaga
     7501 acttttgtcg gaaacgacat gctcactgcc gtatctgccg attctttttt tcaatctgcg
     7561 attgctcaga gagtgaatga acgtgctgct gatacacaaa tgctttttgc acaccacgga
     7621 ttgactcttc gcgataagaa agcacccagc gtcttctttg tcaaaaaact gaaaaataca
     7681 ttgaatcacg tttacggata tgctgtttta tcgatctcat ccaagcaact agcgaatctt
     7741 tttcaatctg tagtaaatcc tgagatatcg aagatctcct tcatgaacga agaacagacg
     7801 atcatcgcat caaatgaaga aaatacaatt gggagccgat caatgatttt caaaaaacta
     7861 acagtaggtg aaacaacgat ttcaaatgga cagcagttga cccgtcttcc tctgtaccgc
     7921 caggattgta cattactcag tcaagtcgat cttcattctt tatctaacca aatgggcgtg
     7981 atttttccga ttattttgta taatatcgtg acacttgctt ttgctggctc attggtattt
     8041 atgtatttgg atcgacacac aaaatcgatc taccgcttga ttgattcgct aaaacggcta
     8101 gaggaagatg gcttaaaagc tactgttcct gtacaaggaa gccatgaagt ccaattgcta
     8161 gggaatacga tcaatcaatt gctaagtaat gctcagcatc attatgaaca gacgatccaa
     8221 gacgaaaaga agaaacgttc gcttgaaatc caaactatgc aagcacagat acagccccat
     8281 tttatttata atacgctgac tagcttgaaa tttttgattt ggcagaaaaa aacagatgaa
     8341 gcagttcaag gaatcgacag tttcattact ttactaagaa gtacgattgg gaaaaaagaa
     8401 gaagtgattc cagtcaagga agaaatcaaa agcgtacaaa gttacatcga tcttttgagt
     8461 atgcgatatg gcgaaagaat ctcggctaaa ataatgattc ctgatgaact gcagtcatta
     8521 tctatgccga atatgatcct tcaaccaatt atcgaaaacg cctatcttca tgcttttcac
     8581 aataaaacgc aaggattcat cacggtgtac ggcaaaacaa aaaatcagct tcttttattc
     8641 gaagtgattg ataacggcga tggcttcgat actgaaaata ccaaacaaaa aaatgatcat
     8701 ttttcaggaa tcggcatcag ccatgtagat gagcggatac gcctaatgta tggcgatgca
     8761 tatggcttaa cgatccaatc agtgattggg acaggaacga ctgtaacgat ttgtctaccg
     8821 cttcttccta aaaatcaatc agaatatcaa aaataaagaa tagccaagcg ctgatttttt
     8881 tattttaagt taaataaatc aatcagtatt cttgactttc atctacgcgt ctcttctact
     8941 gtcagcactg gataacagcc caatatttga ctttctcctc cgagcatctt gatttcttgg
     9001 agcgcattct ctactaatgt cattggacgg tccaacaaca aatcgatcac gaaaaaatat
     9061 tctcccaagc tagttttcaa tggtcgggat tcaatcttac ttaagttgat ttcccgccag
     9121 ccaaaagctg ccagcatttt atgaagcatc cctggccgat tagcaggtag tgtcaagatg
     9181 acactcattt ttactggagc tgactgctgg ctggtcatct ttcggtcacc gacgatccaa
     9241 aaacgtgtct ggttcaactc attatcttgg atattctcag ctaagatctc caatccaaca
     9301 tgctgtgctg tttctaatga agcaatcgct gctgcgtctt ctttcggatg ttctgctaca
     9361 tacattgctg cagcagttgt tgattctgtt gctactaaag gaacattagg ataatgcgtt
     9421 tctaaaaact gttgtgattg tgctagtgct tgtggatgtg aaaggatttt tgtaatcttt
     9481 gttgctggat tgcctaaaag ctgctgcttg ataggaagaa cgatttcact tttaacttcg
     9541 acttctggat gtttggaaag aagatcgatt gtgtgatgaa cagctccttc caatgaattc
     9601 tctacaggaa cgacagccag atcgacttgt ttgcgaaaca aagcgcgcaa acaaacaggg
     9661 atggaagcat acgcagtcaa ttgttctgta ggaaatagct gacaagccgc ctgataagtg
     9721 aatgaacttt ctggccctag atagctgacg ttcatatgct tttgatcctt tctataatgg
     9781 tatcgatgat cttttctggt gtttgattgg tcgtgttgat tcgatgtgtc gcactttctt
     9841 cataccaaga aagtcgttgt gccaataatg cttttatttc ttctcttgtt tttccaagag
     9901 ctaaaggtcg aatagacgtt tcatcttgtt ggatacgatc aactagacag tccgcttctg
     9961 cctctaagta aagaacaatc ggctgtgttt ttaaaaaacg ccgattttct gcactttgga
    10021 caatccctcc accagtcgcg ataattttgt cttcatttga cagctttctt aaaatatctg
    10081 actccacttt tcgaaagctt ttttcaccgt aatagtgaaa atattcggca atcgacaatt
    10141 ggatatgttc ctcaatcttt gtatctaaat ctacatactc tttttgtaaa cattgagcta
    10201 accctttacc gattgttgtt tttccagcgc ccatgaaacc aatcagcaaa atgctagcca
    10261 tgagcttctc ctcctgacac aagttctgct aaatcgtcaa aaaatgccgg ataagagatt
    10321 gaaacagctt cagctccttc tagttcgcaa ggtccatcag ctatcaaagc agctacttgc
    10381 agcatcatac cgattcgatg atcgcctctg ctggacactt ttgcgccatg cagaggtgtc
    10441 ggcccatgga tgatcagacc gtctgctgtc gcttttatct ttgctcccat tttctgtaat
    10501 tcttctgcca ccgcatcaat tctgttggtt tcttttactt ttaattcttc tgcgtctttg
    10561 atgatcgtga ttccttctgc ttgagtagcc actaaagcta gaatgggcaa ttcatcaatc
    10621 agacgcggaa tgatttctcc accaatcgtt gctttcttca aatgactcgt cttgacactt
    10681 aaatcagccg attggttctg ttcattgtga ttcgtctggg taatcgctgc gcccatctcc
    10741 tccaacacat ctaagatacc agttcttgtt ggattgaccc cgacattttt taacaacagt
    10801 tggctttccg gaacaagaag tcctgctgct agaaagaaag cagctgatga aatatcacct
    10861 ggaacagtta tctgttgacc agttaatttt tgcggtccgg tcactatgat cgttttatct
    10921 tccactgtga tccttccgcc aaattgacga atcatttctt ccgtatggtt acgggaacgt
    10981 tctttttcaa cgattacgga tgtgccttct gcttgtaatg ccgcaaacag caaagcagat
    11041 ttgacttgcg cactggctac aggcatattg tatcggatag gtgataaaga gcgggttcct
    11101 gtcacagtga ttggtagata ctggtcattt tcttctcctt gcaagtcagc gcccatctcg
    11161 cgtaaaggtt ccatcactcg ccccattgga cgtttagaca acgaagcatc cccgaacaga
    11221 gtagtcgtaa aaggctgtcc agccaaaata cccatcaata aacgtatggt cgtaccggag
    11281 tttcccatgt caattgattc ggttggttgg acaaatgagt ggctaccgcg accatgaatc
    11341 ttgatcacct cttcctcttc ttcgatctcg acacctaatt gttggaaggc atgtaaagta
    11401 ctcagacaat cttctgcgcg caaaaaattt tgtatagtcg tcgttccttc agcaattgct
    11461 ccaaacatga tgctgcgatg ggaaattgat ttatctgctg gtatcctaac agtccctctt
    11521 aatccatgta tttgttgtaa taattgcatt tatccagcct cctatttgat ccagcaatga
    11581 taactggtat tagacataat gattcttttt gcttgtaaca aatcctgttg gtttttaaaa
    11641 ctgatttgca gtattcctaa aatatcttcc ctggtctctt ggatcttcaa attaatcaaa
    11701 gaaactcccg cttttccgat cagtccagtt acttctgcaa taacgccagg ttcatctggc
    11761 acatcgataa ataaatcaaa aaaggcagga atcgcgcctt gcttatggac aggcaactgg
    11821 tctcgacttt cttttgcccg attgaaaaaa gaaaagattt ccgattggtt ctcagtcatg
    11881 atccattctg ctatttgtgt catttcttgt tgccaatctg ccaatcgttc caataatatc
    11941 tgcttattgc tcaacaaaat atctgtccac atgacaggat ctgaggaagc gatcctagtg
    12001 atatctcgaa agcctcctgc tgccagttgt ttggctcgcg gatactcttg attgaattgt
    12061 tctgcctgat tgactaagcc agctgccaga atatgtggaa aatgacttag cattcctgtt
    12121 atacggtcat gttctcttgc tgtaagagtt acatattttg ctttggttcc gcaaaatagt
    12181 tcttgcagtt tttcaacatc ttttttcgtc ttttccaaag gtgtaaagat gtaataggca
    12241 ttttcaaata gtttagcatc tgctgcctgt atacctgatt tatgagaacc agccattgga
    12301 tgtccaccga caaaccgaag atttttctgc tcagcggatg tcacgacttg ctgcttcgta
    12361 ctgctgacat ccgtaatgat gacgtgatca gacaagttga ttctttcaag gacatcaaga
    12421 taagctaagg ttgtacggac gggaacggct aataagataa cgtctgcttg ttctgcacct
    12481 gtttcaaaat cacttgcaac gtaatcaacg atctgctgtt ctactgcttg ctcacgggtt
    12541 tttgactgac tatcccaacc aactactatc agttctggat tggtctgacg aatacaaagt
    12601 gccatagaag caccaatcag tccaagcccc acgataaaaa catgttgtgc catcttattt
    12661 gccccctttt tagtaatgtt tcaagtagtc acggtactcc ttcactgcag ctttcaactc
    12721 aacaagcgaa tcacttggga attttgacaa tagctctgtc gctaccacag tggctacaac
    12781 attttccgca acgacgctag ctgcaggaac tgctgtacta tccgaacgtt ctacacttgc
    12841 tttgtaaggt tcttttgtat cgatatcaac actttgaagt ggcttgtaca atgtaggaat
    12901 gggtttcatg accccgcgaa tgacaagtgg ttcgccattg gtcattccgc cttcgaagcc
    12961 gcctagatta ttggtacgtc ggaaatagcc tctttcttct tcccatagga tctcatccat
    13021 cacttctgag cctggtaatg aaccagaagt gaatccgacc ccaaattcta cgcctttaaa
    13081 ggcattgata ctaaccacag cttgtgcgat tttggcatct agcttcgtgt cccattgtac
    13141 gtagcttcct aatccagctg gaacgcctcc aacgagcact tctacaattc ctccgatcgt
    13201 atctccattt ttctttgtct gatcgatcaa atcttttatt ctttgctcaa ctgctggatc
    13261 aacaactcgt acttctgacg cttcagaaat ttctttgatt tcttgaatcg agatatttcc
    13321 aggtatttct gcttggattc caccaagaca aatcacgtgt cctgctacga tgatgtcaag
    13381 ttcttccaga attttcttgg caattgcgcc gattgctaca cgcatggtcg tctctcttgc
    13441 agaggaccgt tcaagcacat tccgaagatc tcgatgatgg tatttcatac cgcctaccaa
    13501 atccgcgtgt cctggacgcg gacgtgcaac tcgccgcagt gattggtctt tttcaggcac
    13561 ttctgctaca gacatgacag ttgtccaatt tttccaatct ttattttcta ccactaaagt
    13621 gacaggtgaa ccaattgttt tgccgtgacg gatgccagac gtgatccgaa cacgatcttt
    13681 ttcgatcttc atccttcctc ctctgccgta accagcttgt ctgcgagcga gttcgtgatt
    13741 gatttcttct tctgacagag gtaatcctgc tggtagtcct tcaataatgg ctgttaattc
    13801 tggtccatgg gattctcccg cagtcatata acgcatctta cagtcctcct aagtaatctt
    13861 tgaatgtctc tgtgggaaca ggaacgattt ttgcttgccc aatcttttct agcaagatga
    13921 tattcaattg gcttcctctt gctttcttat catgggttat cgcgtggaat aattcatctg
    13981 gatgacttag atcgtacgta atcggcagat ggaatttttg aagtatcctt tctaaagcag
    14041 ctgtcgatcc agttggagac aagccgattt tttctgcatg agaagtgatg gctaccattc
    14101 caatagctac tccttctcca tgactgacta ccccatatcc tgctgtattt tcaattgcat
    14161 ggccaattgt atgaccaaaa tttaaaataa gtcgatttcc ttggtcaaag acatcttctt
    14221 ccactacttt tcgtttgatt tccaacgtag cagtaatgat tggaacagca tgttccagaa
    14281 gatctttttc gtctttcagc tcgttcagct gtttccaaag cgttgcatct gctatagctg
    14341 ctgatttgat gatttcagcg attccttctc gtaaacgacg taccggcaaa gtttttagaa
    14401 cctcaggatc aatcaataca ccagctggct ggctaaatgt tccgacaagg tttttcgctt
    14461 tgtttgtatt gactgctgtc ttcccgccaa tactagaatc tacttgtgca agtagcgtag
    14521 tcggaatttg aacgaaatgg atcccacgca tgaacgtcga ggccacgaat gctgctagat
    14581 cacccaccat tcctccacca agtgcaagta tcccatcgct gcgggtaaaa tcattatctg
    14641 ctaaaaaatc gtataactca gctgcttgtt ctaaagattt actggtctca cccgccggta
    14701 cgattgaaag gactggttca aaattagctt gtctcaattg gaacatgatt ttttctgcat
    14761 atagtggagc aacgtttgta tcagtgatca cagctacttt ttgaggtctc caaatcgttt
    14821 gaagccatga tccagcctgt tctagtgcgc ctttttcaat atgaatatcg tatttttggt
    14881 tatcaacatg tacagtaagc attcgtttca cttctttctt ttaaaaagct ggaattaatc
    14941 gagtgtctga atcttttcca ttgcttcttt catgatcgca acttctttca tcatgcgtga
    15001 gtaagtcttt tcattcaacg attggggacc gtctgaccaa gcatttgcag gatcaggatg
    15061 gatctctacg atcatgccat ctgctccgct tgccacaccc gcacgagcca ttggtggtac
    15121 aagttcccaa attcctgtac catgacttgg atcaacaatc acagggaaat gagttaattt
    15181 tttcatcaat gggacagcac ttaaatcaaa cgtattacgt gtcgctgttt cgtaagtccg
    15241 aatcccacgt tcgataaaaa tgacaggaga tttgtcttcg actgcaatgt attctgcagc
    15301 attaagccat tcattgatcg taccggaaat tccacgtttc aatccaacag gtttacctgt
    15361 tttaccgaca gcagatagca gtttaaaatt ttgcatatta cgtgcgccaa tctgtaggat
    15421 atcgctgtac tcagccacca tatcgatatg cccttcatcc atcacttcag taatcacttt
    15481 cattccaaat tcatcagccg cttgacggat gtatttcaat ccttcttctt ctaaaccttg
    15541 gaaagcatat ggagaagtac gtggtttgaa ggcaccacct cgcagaattt ttgcgccccc
    15601 ggctttggcc attcgcgcac attctctgat ttggtctaat ccttcgatag aacaaggacc
    15661 agccatcgtc acgaaactgc cgtcaccaat ttttactccg tctacatcca cgactgtatt
    15721 gccaggatgg aattctcgag aagtcaattt gtatgtgttt aaaatacgca cagcattttc
    15781 tactccttca tagcgtgtaa atgctccttc ttgtaaactt cgcgtatcac cttttagtcc
    15841 aatcactact cgttctgttc cttgatcgat tgagactttg aatccttctt tttcgatatg
    15901 atcgacgact ttttttattt gttcttccgt tgcttctttt ttcataatta ctatcattgt
    15961 ttttcccgct ttcttttgtt tacttatcta tcttggctga ttttctctac tttttcaagg
    16021 tggttttaca ttaattccta ctaaatgacc gtgtcctgtt gttctcattt tcgctccttc
    16081 tttcctactt atgacccaga taatcaacaa aaaaagatgt ccctgtagga aattgcctct
    16141 acagtgaaca tcttcaacga gccttaaaaa gcgctgcaaa aatataaaat agccactata
    16201 gatgaatttc tctacgtggc tgtaagtcgt gtttaaaaat ctttagccat catagataca
    16261 aacctggttt gtttgtaccc acgaagtcat gaatacaaac ctatctaaaa tagctaaagt
    16321 aatacgtatt cagttttgta agttttctag atttcataaa aaatcgctcc aaactgatgt
    16381 aagaattgta cctagtatag acataaaaaa atcgactgtc aatcattttt atgaaacttt
    16441 ttttaatttt ctgatagtta actttgtttt tcagaaaaat aacaattggt tttattgaag
    16501 aaaatagtca tgaacaaaag tatctgagaa cttttttact ctcttgttca tgacatatag
    16561 aataaagcag ctttgtcttg taaactccgt ttttcaaata ataatctaaa gattgctact
    16621 gaataataaa gccgatttca tggtcctctt cgctgctgat tcggtaaggt gactcaacat
    16681 ccgctcccca gctattgata cccccgactc ctcttacttt tgcaaaaatc gtaagaaccg
    16741 ttcgtctttc tggcggtagt tcttctatat gtgttgcctg ttccagttct tctgctttga
    16801 atggtaaagc ggaaaaagca aatggcgtat cctgctgttc aaatcgcaac gaaaaatctt
    16861 cttgatcttt atccgtatta tccaacgttt gatttcttgt tagttctacc catttcgttt
    16921 ccatatgcat gccgcattct tggggaacaa ggtatgggga aacggggagt cctgccactt
    16981 tataaatacc ttcgacacca ccagccatcc gatccggata tgtttcccct gataggcctt
    17041 catactcaaa aaattttgct ttcgttggca taatgaacct taaacccaat gcgggtaatt
    17101 ccggtaatcc tttttgtcca taataatgca tattgacaag cattttaccc tctccagcga
    17161 tttggtagga aattgtcact tgggtttttg gtactgtgtt gatttccagt gtaaaaatga
    17221 tttcaaccgt ttcagcgtat tcattacttg aatattgatt atttactgga gcaatcggca
    17281 aatcgatctt tttattgtcc accagtactt ctatttcttt gacttttgga tacaatccgg
    17341 ctcctaacca cataccggat ttaaatccaa atccgctacc tctgtcatta tcggtcgttg
    17401 ctcgccaaaa aactggaagt gtttcccggt atagccattc ttttccgttt ttattgagcg
    17461 attccagtcc gcctcttgta taagaaaaaa tataatgcgt attccccgat ttgacaccta
    17521 atgtacagtc gccgaagacg attgcaactt tttccgtatc catatgactt ttatttactg
    17581 tatttttcat aataatatct tacctcctgc atggcgggtt tttcttgtcc atctgcaaaa
    17641 acgatcccat tccctgagaa ttcatagtca cttggtttat cgtcaaagtc accgccatag
    17701 cgcatcactt ctctgcctgt gattggatca gtcacttgga tcgcctgatc tttaaaatcc
    17761 catatatagc ctccttggta catttcataa cgatcaagca acgtcatgta agaatccatt
    17821 ccacctaacg aattccccat gtcatgcata tattcacata agatgaacgg tttttccgct
    17881 ttattttcaa ggtagtcaat gatctcttct ggtggcgcat acatgcagct ttttacatct
    17941 gaaatcgaat cttcgaatgc tggatttgga aaattccctt cataatggac aagtcgatcg
    18001 gggtctactt ttttgaagta atcgttcatt tgtcgaatgt tttcttccgc ataagattca
    18061 tttccaagag accaaaaaag aatacttgga tggtttttta atgtttcgaa attcgttttt
    18121 gctcgatcaa gaacagccgc ctgccactga ggtactgcac ctggtacgtt gtaagaaggc
    18181 tcaaatgcgc ccattttttg ccatgagcca tgggattcga ggttcgtttc cgccatcaca
    18241 tggattcctg cttggtcgca taaataatac caagtgatct ggttggggta atgagatgtc
    18301 cgtacgccgt tgatctttgc attttttatg atagccaaat ctttttccat atcttctata
    18361 gtgatggcgc gtcctttttt cggatgccac tcatgtcgat ttaccccgtt gataatcatc
    18421 cgttttccat tgaagtagat gattttatct ttgatttcga ttttacgaaa accgaaggaa
    18481 tagtcaatat actccgttac tcttttttct tcgtctatcg tctccaattc aagcttatat
    18541 aaattgggct gaaaatgccc ccacagtgcc acattttcaa atccatcgat gcgaatcgtc
    18601 atttgctctt ttaacggata ttgctgttga tagatttcct tcccatcagg atctagtatc
    18661 gctacattca gctgactgtt tttggctact tgctcaaaag acaaatctac ttcaagtttg
    18721 ccattttgac atttgtcatc taagcgaggt ttgatccaaa gatcttctaa atggttttgt
    18781 cctcgagcaa ctaagaagac atctctgaag atcccaaaaa atcggaaaaa gtcctgatct
    18841 tccaagaatg cagctgtact tcttttgtaa acctccacag ctagaatatt ttcttcttcc
    18901 aaataaggtg ttaaatcaaa ttcagacgga gtgaacgaat cttcggcata cccgataaaa
    18961 tgaccattca accaaataaa cattgcttgt tctacaccag caaaatgaat cgagatttgt
    19021 tttcccttta gaccttcttc taatcgaaaa gtcgtcaaat acgaaccaac cggattgtct
    19081 ttagcagctg aaaagatccc ttccgcctct ttctcagggc ttgtcgtgaa agggggacgg
    19141 cgaaatacat gtccctccca cggatacatc gtattgatgt aatggatctt gtcataattt
    19201 gctaattcaa tatgactagg aacagtgatt tcatcaaaag aacaggcatc aaaatctttt
    19261 ttataaaatg tttttggtcg taccgaagga tttggcgaat aacaaaaacg ccatgatcca
    19321 tttaaagact gtttaaggga ggatgtggtt tctacttctt cccttgttgc ataacaaaca
    19381 tgatcgctat gtgcatttga ttggttcact ctaaaaactt gtggatcatc taaccatttt
    19441 atatttgctt tcatactttt ttccttctct ctcataactt tttgaaatga agcagttctg
    19501 tttggaaatc accttgaaaa tcggggaagt aaagtccaac tgtcatcaat tcggcccccg
    19561 aaaatatccg cttcgaggtc tgttcctgat aaagtgcctc atcatctaac cccatcagat
    19621 atacttcatg gaacgctggt tgagctgaag ctagtatcct ccctaaaaac acaatagctt
    19681 catcttgttt aggagacaca aacatccatg cggcaatatt ttcttcaaaa ggactcgcca
    19741 aacgataata atggccatac tggatcaccg gacgaatcgt ttgataggat ataatttggt
    19801 cttttacttg ctttttttct tcaggagaca gctttgtcaa atccagttca taaccaaaaa
    19861 cagcgctcat agctgtttct cctcttgttt tcaacgaagt agatctgtcg gtctgatgat
    19921 ttggtacagc tgatacatga gctgtgatgg acgaaatcgg ataagcaagg cttgttccat
    19981 attggatttt catacgctcc actgcatccg tgttatcact tgtccatgac tgcggcatat
    20041 aataaataaa cccggcatca aaccgtccac ctccaccaga acagccttcc cataagattt
    20101 ccggataatc agttgtcaaa tgttcaagta gttcataaag cccgagcata taacgatgcg
    20161 ctacttctcc ttgtctctgt gggtcaaggg taatcgaata gacatctgaa agcgaacggt
    20221 tcatatccca tttgatgtag tcaagaggaa tcgtatctaa aatctttctc atttgtttct
    20281 caatcgtctg ccgaacatct aaccttgtaa aatccagtac atgttgtgat ctggaagcag
    20341 aaggcattct acctggctcc tgcataagaa aatcaggatg tgttcgatac agttcagaat
    20401 caaccgagat catttcaggt tcaaaccata aaccaaattt caatcctttg ctatgagcat
    20461 aatctgctat ttcacgtaat ccatttgtca acttgccttc atactcgaac cagtctccta
    20521 atgaagaatt gtccgcatct ctttttccaa accagccatc atctaatacg aacatttcga
    20581 tgcctagctc ctttgcttca tcaatgatcg ccttgatttt ttcactattg aagtcaaagt
    20641 aagtggcttc ccagttattg accagaatcg gccgttcagc aaattgatga cgacctcttg
    20701 cgattctttc acgtagcaac tcatggtgga tctgactcat tttgttcaat ccctgacttg
    20761 agtaagaaag gatcatttca ggtgtttgaa aagactggcc tgcattcaat tcccacgaaa
    20821 aacgatggct gttgatccca ccgacgactc tcaattgatt gatctgatct ttttcaagtt
    20881 cgaaagaatg gttgccggaa taaacaaatt gaagtccgat cgcttctcca gtattttctg
    20941 tcgtatgatg atgaactaaa gcaataaagt tattcatatg gtgactgctc gttcctcgac
    21001 ggctttcaaa tacttttgtt ccagataaaa cagattgccg tgaaatctgt ctttcattga
    21061 catgtgctcc tggcaaagca atgacttcat caaatcgcct agtatgggca aagtctagct
    21121 ggaaactagc cgctttttct agtttgattg tttctccggt ttcatttctt atctttacag
    21181 atcgtgtgat cactgctcta tcagtaaata tggtatagaa caaatcgaaa taaatcgctg
    21241 tttctctatc cttaagtgaa atcaccaacg tttgtgcctc tgacttgtcc atcacataag
    21301 tttgaggcaa gccttcgatc tctggcttcc catctgtaat ataatggctg tcatatctca
    21361 gatccagtaa gtctgatcca ttcttgcgct gaatgatcga tgcgggcgtt cgaaagtcca
    21421 tgctgccatg agaagagtat tcttgcggta gtgtatcttt cgacagagac cggtcaggat
    21481 ttaagggaac atttcctgaa aatccacgat ctcttcgggg ataattttta ttgtctttgt
    21541 actgcttgac tggtttgcca aaataaatat gcgccaatac attgctttct tctatcttta
    21601 tcagataaga aagaaatgta tttcgtaaat ggaagactcg gctcttttca tcaaataaga
    21661 tgaaattttt atctgttttt ctatgcgtaa tatccatcat ttcactccca acaaaacgga
    21721 aaccgtttac ttttttttat aattcctgta tactgtttac taaacaattt tgtcaataaa
    21781 aattttttat ttttttgagg agtgtgtaaa aatggttgga attagagatg tcgcacgaaa
    21841 ggcaaatgtc tctcctgcta cagtttcccg tgtgttgaat aacgatcaga cgatccatgt
    21901 ggctcagact actcggcaac ggatagtaga ggcagctgct gcgttggatt atgacatcag
    21961 tactcgtaaa tatatcaaaa agagaatgcc ttcgattggt gtgatctcta cgatcggcaa
    22021 agcgagcgaa gaacaggata tctattacaa acatttgcga gtagggatgg aagaagaagc
    22081 tcgacgtctg catttaggga tgaatcgaat ctataatcta tctgataatc ctaaacaatg
    22141 ggaagatcta gatcagttag gtgcggtgat cgttgttggg actgttacga aagaatcgat
    22201 taccgccttg ttaaaacaaa acatgcaaat agtcgtcgtg gataaccctg acatccagca
    22261 agaggtcgat atggtgtatg cagatcttga acggatgact aaaaccgtac tggaattgtt
    22321 cttagaaaat ggtcataaaa gaatagccta tatcggggga tataaagtcg atatggatga
    22381 attcggtggg aagcagattt ctataaatga aaagaggatg cgtgcctatc agcattttat
    22441 gatcgatcat cacttagaaa aatatatgtc ttatcatcta ggtggctgga cagaaaaaca
    22501 aggtgaggta cttgcgacta atctattaga aaatgaatcc cctcttccta ctgccattct
    22561 tgtcgggagc gatccaatgg caattggtgt ctatcgggca ttgaaacaaa aaaatatcca
    22621 aattggagaa gagattgttg tagcaagctt tgataatatc gagacaatcg caaacctctc
    22681 ccctggcttg accagtgttc aaatcaatgc gaaatcaatt gggcaagcag ctgttcgaat
    22741 ggcattggag cgaattgacg gtatacggga cgaaccaatc attttgactt atcctgcacg
    22801 attgattgtt agggaaagtt ttatcccaag aacaaaaagg tattgaaaga gaggttgtga
    22861 aaagccgctt cgtttcttca tactcaaaac gaaacggctt tttcacaacc tcttacttat
    22921 acttattcac aaaatccaat gtctgctggt aacaattttg cgattctttt agcgtgtaat
    22981 caaattggta ttcatgggta atctcttttt ttgtatctgt ataaaacaag gattgcacgg
    23041 gtacctttaa ttctgtcaac cgattttcta atgcttgacc ttgctcctgg aatgagtatg
    23101 catttccgtc agttatatac gttggcggaa attccttaga aacatggtca gccacactag
    23161 cttctttcaa ttgctcactc gttttccaat cttttgttcc aatcaacgac cacgcaacgg
    23221 ttttgacgaa aaacttcata aatcgatcat ccgaatgctg ctgtgctgtt agcttcaaat
    23281 caacaggtcc ccaataagaa atggttcctt tcagactatc ttttggaagt agctgtttga
    23341 ttttcatttg tttagcatac gcatcattcg tttgtatagc tgcatattgc aatgcgattt
    23401 gtgaacctgc actatctcca ccaaaaaata attgttccat gtctagcaat ttgtcgtctt
    23461 tttcttcagc taataaatct ttgatcagtt cattcacttg aagaacttga ttagggtatt
    23521 ctgaatcagg cgctaattca taattcatcg caaccactgc gatgttggcg tctgaaacta
    23581 attttgtcgc aaattctttg acacccgatt tatctccgcc gacgaatcct ccaccatgta
    23641 cccaaaatag aacaggaaca ggccccttgc tgttttttgg atagtagatg tcataagtat
    23701 tctctttata cttggactca tacgtcaaat ctgttttttt cgccacgttt ttttctgctt
    23761 gatcgaacgt ttttttatca gtgatttgaa cagtatttga aaacatatgg ttgatcaaga
    23821 atgctccagg tctgggggaa agctggaagg caagaaagat tgcaaacgcg atcccaagaa
    23881 tgattccgaa gaaccatccg ataaatttaa gaattttctt tttcataagg ctccctcagt
    23941 agttagtatt aagcattctg attataccat agtgttattt cctatcaagc tctcctagat
    24001 gatttaatga ttttccccaa acttcttctg caatgctttt gacaaaagaa attttttcca
    24061 ttgttcttca aacaaaacat tttcttcttc tgttgaagca aatccacatt gaggacttaa
    24121 gcacaaacga tccaaaggca gatatcgtgt cgcttcgtag atccggtcaa tcacttgctg
    24181 gtgctcttcc atttcgccat ttttcgttac agtcaaaatc taagacaact aatttatctt
    24241 cagagacttt ctctaaaaga acaaaactcc cagcacgttc actatcgtat tctaaataat
    24301 aggcacggac attttcttga gtaaaataat gggtctgcta cgtcgtcatc ccccctgatg
    24361 ccgccaaaat cgaataataa tttccgcgtc aaacatgtat atttagtgtt gaatcatttg
    24421 agaatatgaa aataaaaatc tttttgatca atcagttaat caaattgaaa taacgaaaaa
    24481 tctgagacag aatgacgttc attctgaccc agatttgatt ttgcacaact caacagtcct
    24541 tattggcaaa tcgccaaaat aatgtagact aacta
//
LOCUS       DARBET010000035        23885 bp    DNA     linear   BCT 07-NOV-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4027
            SAMEA114569841-rid20112183.denovo.035, whole genome shotgun
            sequence.
ACCESSION   DARBET010000035 DARBET010000000
VERSION     DARBET010000035.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA114569841
            Sequence Read Archive: ERR12203666
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 23885)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 23885)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001972618.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001972618.1
            Genome Coverage       :: 132x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/07/2023 13:18:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,041
            CDSs (total)                      :: 2,975
            Genes (coding)                    :: 2,908
            CDSs (with protein)               :: 2,908
            Genes (RNA)                       :: 66
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 67
            CDSs (without protein)            :: 67
            Pseudo Genes (ambiguous residues) :: 0 of 67
            Pseudo Genes (frameshifted)       :: 26 of 67
            Pseudo Genes (incomplete)         :: 28 of 67
            Pseudo Genes (internal stop)      :: 20 of 67
            Pseudo Genes (multiple problems)  :: 6 of 67
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..23885
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA114569841-rid20112183.denovo.035"
                     /isolate="VRE4027"
                     /db_xref="taxon:1352"
                     /country="Denmark"
     gene            complement(55..789)
                     /locus_tag="SCJ86_001583"
     CDS             complement(55..789)
                     /locus_tag="SCJ86_001583"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723412.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aquaporin family protein"
                     /protein_id="HEG1169124.1"
                     /translation="MDYSLMTRLAAEMIGTALLIVLGNGAVANVDLKGTKGYGSDWML
                     IAVGYGFGVMMPAMIFGGISGNHINPAFTIALASNGLFPWAEVVPYIIAQLIGAAIGQ
                     LIVVACYKPYYDQTEDVNHVLGTFSTINSVGSKFNGFVNEFFGSFVLFFGALGITKAP
                     FFQDNLGTAHLALGFLVWTLVASLGGPTGPGLNPARDFAPRLVHALLPLKHKGSSQWG
                     YAWVPVVAPICAGVVAVFLYKLLFMA"
     gene            1116..1526
                     /locus_tag="SCJ86_001584"
     CDS             1116..1526
                     /locus_tag="SCJ86_001584"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309804.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CidA/LrgA family protein"
                     /protein_id="HEG1169125.1"
                     /translation="MKFFQEFFIIIFFSVLGEGIRIFAHLPIPGSILGILFLFLAFEM
                     HLVDPEKIGATGDFLLNNLAILFVPAGVGLLEYFDDIASIWPVLLGAVVVCSIVTMVA
                     AGKTAEGVEALLGYVRKKKAERVSVNEMEENQID"
     gene            1519..2220
                     /locus_tag="SCJ86_001585"
     CDS             1519..2220
                     /locus_tag="SCJ86_001585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005880671.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LrgB family protein"
                     /protein_id="HEG1169126.1"
                     /translation="MTEFFVNPLFGLSLTLGVYVLSDKFLRKYKLPVLNPLVLSIVVI
                     ILFLHFSGISYKDYNIGGNFLTMLITPATVALAIPLYKNFRLLKENFYPVMAAILAGI
                     VANCLVSVGFGYFFSLHKEMVISLLPKSVTTAISVDLSHTMGGVNAVTLAIVVSTGIF
                     GSLIATHIFRLFKIESPVARGVALGSTSHAIGTAKAIEIGEIEGIISGLAICVNGILT
                     VLLLPLFFQPFAGLF"
     gene            2220..3806
                     /locus_tag="SCJ86_001586"
     CDS             2220..3806
                     /locus_tag="SCJ86_001586"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002358389.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HEG1169127.1"
                     /translation="MRKPLFYATITKTFEHKKGGKDVYLSSEYLKQFDKDLYYKILLL
                     ESFEDQAWHTAAQLAQVVQLDARSVSKYLNELSKNYQQFSGKTHPLFTKNHRSGYNFY
                     DTLDSIEHERFLIYLVQSTLKFQLLHDIFFEEFHTMYQFAQKHYISESTAHRKINEWK
                     QQLQTYGIRLQRGTYIAQGEEEIIRLYLHMTFWQLFRGKIWPFETISQMDVKNMAEHI
                     MAFFNVRLNEIKKRRLEYMLGAFFLRKSQKHYVVLNEKKRRLISDNLLFQRFCQVMEP
                     VFPNYFQVEDELGALFLVLMTREEYYSDPKIRKKIFDFHQQAKTPPFTALSEAKAALS
                     LYQEEQGLPAENLTFEAENYLFSSHFFAYLFPNAKETIDGNSSDFINHLVIENKELKQ
                     WLVHFFESRHKHPNHLAFKNHAFLMGRYLTVFKTLGAFTPQLPKITILLMTDFPLFEE
                     QLLEEGLRTFFRNEYQLIFLPTDYRGREVDLLISTSKVHRKPWADLDYFIVTEELKLI
                     DYIQLSQKFEMIQKQKLLKQ"
     gene            3825..4046
                     /locus_tag="SCJ86_001587"
     CDS             3825..4046
                     /locus_tag="SCJ86_001587"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016186310.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2829 domain-containing protein"
                     /protein_id="HEG1169128.1"
                     /translation="MTFEEILPELKKGAKIIRKGWSGFELYVVLVSDDEFDGCPVTPY
                     LLIKTSDEGFSSFAPTVCDILADDWAIVE"
     gene            4043..4804
                     /locus_tag="SCJ86_001588"
     CDS             4043..4804
                     /locus_tag="SCJ86_001588"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002372559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-oxoacyl-ACP reductase"
                     /protein_id="HEG1169129.1"
                     /translation="MKNQYPDLAKQVVFITGAASGIGAAQARAFLEQDAFVFGLDQKF
                     GKMEEISLRFPETFAYALGDVRQMKELQQAVSKCQNHFGEVTILLNTAGILDAYKPLM
                     ETDESLWDLIYETNVKSMYQLTKIILPDMIRQKSGTIINMASIAGLIAGGGGIAYTSA
                     KHAIVGFTKQLALDYAGQGIRVKGIAPGAIQTPMNAADFEGKGEMAEWVANETPVKRW
                     AQPEEVAELTLFLASPQASYIQGTIIPIDGGWLLK"
     gene            5184..5693
                     /locus_tag="SCJ86_001589"
     CDS             5184..5693
                     /locus_tag="SCJ86_001589"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="QueT transporter family protein"
                     /protein_id="HEG1169130.1"
                     /translation="MEKNNTRSAKRWTTTDVTRMALVTGLYVAVTLVLSVFSFGVIQI
                     RLSEMFNYLSLYNKRYIVAVTAGVALANIASPLGLIDVAVGSVSTLLVLLINYKITSR
                     IKNMKIKMAVTACVFAFSMFTVAGQLTILYQLPFFLNWWVIALGELASMIVGGIIIYW
                     IGKKVDLSK"
     gene            complement(5768..6307)
                     /locus_tag="SCJ86_001590"
     CDS             complement(5768..6307)
                     /locus_tag="SCJ86_001590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294267.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biotin transporter BioY"
                     /protein_id="HEG1169131.1"
                     /translation="MNRTLKELILAAEFAAIISVLSQMTIPFGLVPLTGQTFAIGLTA
                     TFLGRKLGLISVGVYLLLGLIGLPVFSGMTGGIAVLFGPTGGYLIGFLLQTWITGWMI
                     ERTDAHYLYAIFANLMGSLAALICGTIWLKISGDLTFTTAFASGLLPFLLPEAIKATG
                     AAYIGILMKKRLNRFLHTN"
     gene            complement(6447..7058)
                     /gene="rpsD"
                     /locus_tag="SCJ86_001591"
     CDS             complement(6447..7058)
                     /gene="rpsD"
                     /locus_tag="SCJ86_001591"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734406.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S4"
                     /protein_id="HEG1169132.1"
                     /translation="MSRYTGPSWKISRRLGISLSGTGKELARRPYAPGQHGPNSRGKQ
                     SEYGMQLTEKQKLRHMYGMNERQFRNLFVKASKIKEGKHGVNFMILLEQRLDNVVYRL
                     GLATTRRQARQLVNHGHILVDGKRVDIPSYHVEVGQVISVREKSQNIVTVKEAVEATV
                     GRPAFVSFDAEKLEGSLTRLPERDELYPEIDEALVVEYYNQSL"
     gene            7320..8171
                     /locus_tag="SCJ86_001592"
     CDS             7320..8171
                     /locus_tag="SCJ86_001592"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321201.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate/nitrite transporter family protein"
                     /protein_id="HEG1169133.1"
                     /translation="MRGYVQMEKSEGSLRRKSMNPVSPLFEKIDASIEKKANLFENSF
                     SRYAVRAVLACLFLTLGTAVAFAIAMKGEDIAHGLGKILYAFMFSWGLVMILYMNAEL
                     GTSNMLYMTVGVFRQRLALSKAAKILFACIFFNMIGGILCGFLISMTTPFQDLTLDNF
                     MLESIAVKLAKPSATILVEGMFANIVVNTAVLISMRMKEDVGKVITVVFIIFIFAFLG
                     YEHVIANFPAFTLTYFASHGQMDGMTVSNVLHNLFFALAGNYIGGGLVMGLGYAWLNQ
                     SKSSYVD"
     gene            8199..8834
                     /locus_tag="SCJ86_001593"
     CDS             8199..8834
                     /locus_tag="SCJ86_001593"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294262.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA alkylation repair protein"
                     /protein_id="HEG1169134.1"
                     /translation="MEVLVFPKNEEKALQMEKYMKNLFSFAGVPKPERALLQQPLLDE
                     SKNLSISEVLAFVHYYYEQIEREYQYVAIDLVQKNIKRLTFEDLVVLLPLVQKKEWWD
                     SIDSWRKVYSTWCKAYPEQREEVFQLFEKKEDFWLRRIAITLQLGYKQQTDTGLLIRA
                     IDEDRQTKEFFIQKAIGWALRDYSKTDADWVKRQLTTDLSKLAVREASKYL"
     gene            8854..9627
                     /locus_tag="SCJ86_001594"
     CDS             8854..9627
                     /locus_tag="SCJ86_001594"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853152.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cof-type HAD-IIB family hydrolase"
                     /protein_id="HEG1169135.1"
                     /translation="MRRKLFAFDIDGTLLDSNKQALDSTREALEKLRKAGHLVTIATG
                     RSRFHAQEIIRDLDFTNYILCNGAAGFLDHEQVYKNLLDRDQLDAFVQEAHHEQIDTA
                     FVSLDNIKRSSSNDVTVMEEAMHSFGAVLPELDQFFAEEQEIYQALAFFDQTYDGKFS
                     SYDKIRFVRWHENSVDVVPKNGSKAATILHLAEQVGIPHEDIISFGDGQNDREMLRMS
                     GIGIAMGNAVPEVQAEAKMVTDTNDQDGIWKALKELSVI"
     gene            10035..10832
                     /locus_tag="SCJ86_001595"
     CDS             10035..10832
                     /locus_tag="SCJ86_001595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR00282 family metallophosphoesterase"
                     /protein_id="HEG1169136.1"
                     /translation="MRVLFIGDVVGSMGREMITEYLPRLKKKYRPQVTIVNGENAASG
                     RGITEKIYKKFLQDGVDVVTMGNHTWDNRGIFEFVNEAKKMVRPANFPEGTPGQGMVF
                     VKVNQVELAVINMQARSFMVDLDDPFRKMNELVEEARKRTPIIFVDFHGETTSEKQAM
                     GWFLDGKVSAVVGTHTHVQTNDARILPRGTAYLTDVGMTGPYDGILGMRREPVIEKFL
                     TALPKRFEVVEQGRSILSGCILELDDTTGHAKEIQLIQINDDRPFME"
     gene            10810..11223
                     /locus_tag="SCJ86_001596"
     CDS             10810..11223
                     /locus_tag="SCJ86_001596"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628157.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YlbF family regulator"
                     /protein_id="HEG1169137.1"
                     /translation="MIDRSWSRKRGSFLQQEPEIQKEVRKLTKLLRENETIIRYKELE
                     EKIQQNQYLAELREKIKQAQKDAVHFAHYDKPAAEKEAIKQADQFMQEFDQHPLVVAY
                     RKQLLEADDLLHHLTTMIQEEINGQIEEEKHASKN"
     gene            11207..13852
                     /gene="mutS"
                     /locus_tag="SCJ86_001597"
     CDS             11207..13852
                     /gene="mutS"
                     /locus_tag="SCJ86_001597"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628823.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA mismatch repair protein MutS"
                     /protein_id="HEG1169138.1"
                     /translation="MPQKTKNTPMMEQYLSIKAQYQDAFLFYRLGDFYELFYEDAIKA
                     SQLLELTLTSRNRNAEEPIPMCGVPHHAAQGYIDALIEKGYKVAICEQVEDPKTTKGM
                     VKREVIQLVTPGTVMDSKGLSAKDNNFLTAVVSQGNEFGFAYADLSTGELKTARLHDE
                     EAVLNEASALQTKELVLGSEITDTLREQLSGRLGLVFSQQNEMEENAEFSFLTSELVD
                     PLEKEATGKLLTYLSVTQKRSLAHIQKAVEYQPDHFLKMDYYSKFNLELSQSIRTGKK
                     HGTLLWLLDETKTAMGGRLLKQWLDRPLIQAKQIKARQEMVASLLDSYFERIDLQSAL
                     TKVYDLERLAGRVAFGNVNGRDLIQLKTSLEQVPMIRGLIEGIDQGQWQSLLSEMQPM
                     DHLVQLIDQAIQDDPPLQITEGNIIKDGFNEQLDMYRSAMRNGKKWLAELEAKERQET
                     GIKNLKIGFNRVFGYYIEITKANLGNAELEKYERKQTLANAERFITPELKELETQILE
                     AEEKSVDLEYQLFLAVREEVKKAIQPLQVLAKAISAVDVLQSFATISERYQYVRPELV
                     SDKHQLLIKDGRHPVVEKVLGHQEYIPNSVEMAEDEMILLITGPNMSGKSTYMRQLAL
                     TVLMAQMGCFVPAKQAVLPIFDRIFTRIGASDDLIAGQSTFMVEMMEANQALRYATPN
                     SLILFDELGRGTATYDGMALAQAIIEYIHRHVQAKTLFSTHYHELTVLEKELPQLKNV
                     HVGAVEKDGEVVFLHKMMDGPADKSYGIHVAKIAGLPSNLLERAADILHTLENGDNGH
                     HTVPAEVEEKTKTAKQEQIKPPAEKVNQDHIQDRVKEENQQLSLFSELSENETEVIES
                     LKQINLLEMTPMDALNTLYELQKQL"
     gene            13870..15978
                     /gene="mutL"
                     /locus_tag="SCJ86_001598"
     CDS             13870..15978
                     /gene="mutL"
                     /locus_tag="SCJ86_001598"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628159.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA mismatch repair endonuclease MutL"
                     /protein_id="HEG1169139.1"
                     /translation="MAKIQELSERLANQIAAGEVVERPASVVKELVENAIDAGSTQID
                     ILVEEAGLRTIQVIDNGEGILADDVENAFKRHATSKIHNRDDLFRIRTLGFRGEALPS
                     IASVSEMTLETANQEEKQGTYLSLKGGEVIEHRPAPLRQGTKITVSNLFFNTPARLKY
                     VKTLHTELANIGDIVNRLALSHPSIAFRLVHDGNKMLATAGNGDLKQTIAGIYGLSTA
                     KKMLKIEGKDLDFELFGYVSLPEVTRASRNYLSTIINGRFIKNFSLNKAIVAGYGSKL
                     MVGRFPIAVLEIKMDPLLVDVNVHPTKQEVRLSKEKELTELISNAIQKALSQENLIPS
                     AADNLRFKKKVPDQPKTEQIEIDLSYAFEEEKPKKPSSLNFDRETGTFFVEELKNTFV
                     DRIVDKSVENVENYSGFSEENTTYPEKTAKTVDKFGENVDNFVEKTVEKSEKKEFPIV
                     DNMEKTNEEQADKSVEEDEKIYEEELTLHPEFDFTQGNDKTLEQAIKKLETEKPVQRF
                     PQLEYFGQMHGTYLFAQSSDGLYIIDQHAAQERIKYEYFREKIGEVSDDLQELLVPFV
                     LDYPNSDALKLKEQKHLLEDAGIYLEEFGQNSFIVRAHPTWYPNGEEESIIREMIDML
                     LTTGSVSVKKFREATAIMMSCKRSIKANHYLNEAQARVLLKDLARCENPFNCPHGRPV
                     LIHFTNSDMERMFKRIQDPH"
     gene            16000..16560
                     /gene="maf"
                     /locus_tag="SCJ86_001599"
     CDS             16000..16560
                     /gene="maf"
                     /locus_tag="SCJ86_001599"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="septum formation inhibitor Maf"
                     /protein_id="HEG1169140.1"
                     /translation="MKIVLASQSPRRKELLAHIVSEFEIEPADIDETPFPDEQPVDYV
                     RRMAEEKARTVWEQDEKEDHLVIASDTTVVLNQEIMGKPEDLAEAEFMLKKLSGETHT
                     VYTAVVLKTKEREERILAEAHVTFYPLTDEEIKRYLETGDYADKAGAYGIQNAASVFV
                     KEISGDYYSIVGFPIGAVNQALKTFI"
     gene            complement(16645..18633)
                     /gene="treP"
                     /locus_tag="SCJ86_001600"
     CDS             complement(16645..18633)
                     /gene="treP"
                     /locus_tag="SCJ86_001600"
                     /EC_number="2.7.1.201"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324281.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS system trehalose-specific EIIBC component"
                     /protein_id="HEG1169141.1"
                     /translation="MAKYQADAEKLLHDIGGKENIAAVSHCATRMRFVLNDPGKADEK
                     AIEDIPSVKGMFTNAGQFQVIIGNDVSTFYNDFVAVSGVEGVSKEQGKAAAKQNLHPV
                     QRAIAVLAEIFTPLIPAIIVGGLILGFRNVLEGIQFESLGGTIVEHSKFWNGVNAFLW
                     LPGEAIFHFLPVGITWSIAKKMGTTQILGIVLGITLVSPQLLNAYSVASTAAADIPFW
                     DFGFAQVQMIGYQAQVIPAMLAGFMLAYLEIFFRKYIPQSISMIFVPLFSLLPTVLAA
                     HVILGPIGWTVGSWISNIVNTGLTSSISWLFSAVFGFLYAPLVITGLHHMTNAIDMQL
                     IADFGSTNLWPMIALSNIAQGSAVLAIIFLHRGNKKEEQISIPAMISCYLGVTEPAMF
                     GINLKYVYPFVAAMVGSGLAGMFANLMGVRANAIGVGGLPGILAIQAETWVPFIIAMI
                     IAVIIPFGLTIIFRRQGILNKIDPAVPENAADVQLQTANGATATPQSFEPVSATGTAV
                     ATKETLFAVAAGTIKEITEVNDPVFSQKMMGDGYAVEPSNGKVYAPVNGKVTSVFETK
                     HAIGILSNEGLEVLVHMGLDTVELKGVPFNVFVKEGYLVTPETLIAEMDLPEIEQAGK
                     KTDIIVALTNNEKVAGLSLDQSGLVRPGEAVGKAEVKS"
     gene            18856..19569
                     /gene="treR"
                     /locus_tag="SCJ86_001601"
     CDS             18856..19569
                     /gene="treR"
                     /locus_tag="SCJ86_001601"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002372578.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trehalose operon repressor"
                     /protein_id="HEG1169142.1"
                     /translation="MNKFNEIFLDLEQKILAKEYPPHSLLPSENQLIKIYGVSRETIR
                     KALNLLTTEGYIQKKQGKGSIVLDRNRFDFPISGLTSYKELQETQRIPSETIIHTLEE
                     TEVTKALNEITGWEIGAPVWHLIRERKINGEVVILDTDYLLKEIVPILTPAQAQGSIY
                     EYFENELSLTIAYAQKEITVEEVTEIMKTAMDLHDDTYAVVVRSLVHLEDTRCFEYSE
                     SVHRLDKFRFVEFARRRKV"
     gene            19646..20092
                     /gene="msrB"
                     /locus_tag="SCJ86_001602"
     CDS             19646..20092
                     /gene="msrB"
                     /locus_tag="SCJ86_001602"
                     /EC_number="1.8.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290527.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide-methionine (R)-S-oxide reductase MsrB"
                     /protein_id="HEG1169143.1"
                     /translation="MDKPIRPEKEELKEKLSPMAYAVTQENATERPFSGKYDDFYEKG
                     IYVDIVSGEPLFSSAEKYDAGCGWPSFSKPITKRQVREKADFSHGMHRIEVRSKEADS
                     HLGHVFTDGPVDQGGLRYCINSASLKFIPYEKMDELGYGEFKSLVE"
     gene            20262..20864
                     /gene="ruvA"
                     /locus_tag="SCJ86_001603"
     CDS             20262..20864
                     /gene="ruvA"
                     /locus_tag="SCJ86_001603"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294251.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction branch migration protein RuvA"
                     /protein_id="HEG1169144.1"
                     /translation="MYEYLTGVVTFINPYYIVVETNGIGYQIALGNPYRYSSKLNQKI
                     KIYVHQVIREDAHLLYGFDSLEEKQLFLRLVSVSGIGPKSALAIMASEDHSGLIQAVE
                     SSDVTYLTKFPGVGKKTAQQMILDLKGKFGELSIDTPFSLFDEANTKDATALSEAIEA
                     LSALGYSDREIKRVEKQLKEVDNQTTDEYLRQALKLMMKK"
     gene            20877..21878
                     /gene="ruvB"
                     /locus_tag="SCJ86_001604"
     CDS             20877..21878
                     /gene="ruvB"
                     /locus_tag="SCJ86_001604"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328650.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction branch migration DNA helicase
                     RuvB"
                     /protein_id="HEG1169145.1"
                     /translation="MTDEYLLSPESGENELSLEKSLRPQLLSQYIGQRKVKQELQIYI
                     EAARNREEPLDHVLLYGPPGLGKTTMAMVIANEMTVNIRTTSGPAIERPGDLVAILNE
                     LEAGDVLFIDEIHRLPRVVEEMLYSAMEDFYVDIMVGQGPTAHPVHFPLPPFTLIGAT
                     TRAGMLSAPLRDRFGIISHMEYYDEQDLQEIVLRSSDIFQTEIIEEGALEIARRSRGT
                     PRIANRLLKRVRDFAQVQGDGKIDRPIADQALTLLQVDQAGLDYVDQKLLRTMIELYG
                     GGPVGLSTLSVNIGEERETVEDMYEPYLIQKGFLKRTPRGRIATAYAYEHFGYDYHI"
     gene            21907..22485
                     /locus_tag="SCJ86_001605"
     CDS             21907..22485
                     /locus_tag="SCJ86_001605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HEG1169146.1"
                     /translation="MSDTTRTKQLIKNVLIDLCQTQSFRKISVQKIANEAKINRQTFY
                     YHFTDKYDLLRWVYCEDALHYLDPQCLSLDNWEEQALKMLKAIAEKKEFYANTVLSDP
                     EILQKEFSQLIQKSFIKIFEQMDEERQLSARDKLFYARFFSYGCSGVLVNWITNEYEE
                     TPMEIAMQLFRLAKDTELYSYRLYAKEELEEQ"
     gene            22537..23019
                     /locus_tag="SCJ86_001606"
     CDS             22537..23019
                     /locus_tag="SCJ86_001606"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328037.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="low molecular weight phosphotyrosine protein
                     phosphatase"
                     /protein_id="HEG1169147.1"
                     /translation="MTKILFVCLGNICRSPMAEGLLRKRIALEGREAEFFVDSAATST
                     YEVGKTPHPGTKKILNQEKVDMTNMVARQITPHDFETFDWIIGMDQENVEELKRRAPR
                     SAQGKIHLFLSSIPGKEKENVPDPYYTNNFDETYHLITEGLNHWWNIWIDENKITNNL
                     "
     gene            23136..23510
                     /locus_tag="SCJ86_001607"
     CDS             23136..23510
                     /locus_tag="SCJ86_001607"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628819.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEG1169148.1"
                     /translation="MNEETVSPMKKLTKLVTHTSYQLFDSFVNDTEEHAKNVRLQGKF
                     ILELQKAATEHSLVILQVRANRLSSKYETISGWVATKTVEKDRVMIRLQNEEQQIRMV
                     PVEQIEKITILSPAGEQERLSK"
BASE COUNT         7810 a         4289 c         5123 g         6663 t
ORIGIN      
        1 ataaaaaaac ttggtacgca atcgctgtac caagtttttt tatttagctt ttgattaagc
       61 cataaataat aatttatata agaatactgc tacgacacca gcacagatag gtgctacaac
      121 tggtacccat gcatatcccc attgagaaga acctttatgt ttcaatggaa gaagtgcgtg
      181 tacaagacgt ggagcaaagt cgcgggctgg gtttagtcca ggacctgtag gaccaccaag
      241 tgaagcaact aatgtccata caaggaaacc aagtgctaag tgagctgtac caagattatc
      301 ttggaagaat ggtgcttttg taatacctag ggcaccaaag aataaaacga atgaaccaaa
      361 gaattcattg acgaaaccat tgaatttaga accaacgctg ttgattgttg agaatgtacc
      421 taacacatgg tttacgtctt ctgtttggtc gtagtaaggt ttgtaacaag ctactacgat
      481 caattgtcca atcgctgcac caatcaattg tgcaatgata taaggaacaa cttcagccca
      541 agggaataga ccattagatg ctaaagcaat tgtaaatgct gggttgatgt ggttaccgct
      601 gattccacca aaaatcattg ctggcatcat aaccccgaat ccatacccga cagcaatcag
      661 catccagtca ctgccatatc cttttgtacc ttttaaatct acgtttgcta cagcaccgtt
      721 acctagaacg attagtaatg ctgtaccaat catttccgcc gcaagacggg tcattagtga
      781 gtaatccata aatacctcca aaagttataa aaatgcaaaa aaaaaagaaa catcaacacg
      841 aaaagcatcg gtgcagtgtt tcgtgtcgga aggatttctg cagtcttaac aaatgtgaat
      901 aaaaaaatga attccattac ttataagaaa ccagaccaac agtaaaactc caactctttt
      961 taaaagttct ccacaaatca ccaagcttaa ctataaatat accaaaaaaa aaacgttttt
     1021 tttctcaaaa attgtatctt agaaacagca tgttttgaaa ctttcagaaa aggtttgtta
     1081 gtaaaatgaa agctatacgt aaggagtgtt gtttgatgaa attttttcaa gagtttttca
     1141 ttattatttt cttttctgta ttaggagaag gcatacggat ttttgctcat ttacctattc
     1201 ctggaagtat cttaggtatt ctttttttat ttttagcatt tgaaatgcat cttgttgatc
     1261 ctgaaaaaat cggagcaact ggtgattttt tattgaataa tctagccatt ttatttgttc
     1321 ctgctggtgt aggtttattg gaatattttg atgatattgc ttcgatatgg cctgttttac
     1381 tgggagcagt cgtcgtgtgt tccatcgtca cgatggttgc agcgggaaaa acggcagaag
     1441 gtgtagaagc tttgctgggc tatgtccgta agaaaaaagc agaaagggta tctgtaaacg
     1501 aaatggagga gaatcaaatt gactgagttt tttgtgaatc cattatttgg cttaagtttg
     1561 actttaggag tgtacgtatt atctgacaaa tttttgcgga aatataaact gcctgttttg
     1621 aatcctctcg tattatcgat tgttgttatt atcttatttt tgcatttttc tgggatcagc
     1681 tataaagact ataatatcgg gggcaatttt ttgactatgc tgattactcc tgcgacagtg
     1741 gcacttgcca tccctcttta taaaaacttc cgtttactga aagagaattt ttatcctgta
     1801 atggctgcga ttttagcagg aatagtcgca aattgcttgg ttagtgtagg gttcggttac
     1861 ttcttctctc ttcataaaga gatggtgatc tcattactac ctaaatctgt aacgacggct
     1921 atttctgtcg atttatctca tacgatgggc ggggtgaatg ccgtgacact tgctattgtt
     1981 gtctctacag gtatttttgg ttcgctgatc gccactcata ttttccgcct attcaagatc
     2041 gaaagtccag ttgctcgcgg tgtggcgtta ggaagtactt ctcatgctat tggtacagca
     2101 aaagcaatcg agattggtga aatagaagga atcatttccg gacttgcgat atgcgtcaac
     2161 ggaatattga ccgttttatt gctaccgtta ttctttcagc cttttgctgg cttgttttga
     2221 tgcgaaaacc actattttat gctacaataa ccaaaacgtt cgagcataaa aaaggaggga
     2281 aagacgtgta tttgtcttcc gagtatctta aacaatttga taaagatcta tattataaga
     2341 ttctcctcct agaaagtttt gaggatcaag cttggcatac cgctgcacaa ttagcgcaag
     2401 ttgttcagct ggatgcccgc agcgtgtcga aatacctgaa cgagctgtct aaaaactatc
     2461 agcagtttag cggtaagaca caccctttgt ttacaaaaaa tcaccgttct ggttataact
     2521 tttatgatac gctcgattca attgaacatg agcgtttttt gatctatctc gtgcaaagta
     2581 cgctgaaatt ccagttgctg cacgacattt tttttgaaga attccataca atgtaccaat
     2641 ttgcgcaaaa gcattacatt agtgaatcta ctgctcatcg aaaaatcaat gaatggaaac
     2701 agcaactgca aacttacgga attcggctgc aacgaggtac atatatcgct caaggcgaag
     2761 aagaaatcat tcgactttac ttgcatatga cattctggca gttgtttcga gggaaaatct
     2821 ggccatttga aacgatttct caaatggacg taaagaatat ggcagaacat atcatggctt
     2881 tttttaatgt tcgtctgaac gagatcaaaa aaagacgatt ggaatatatg ctaggtgcct
     2941 tttttttaag aaaatcacag aagcattatg ttgtattgaa tgagaaaaaa agacggctga
     3001 tttctgacaa tctgttgttc cagcgatttt gccaggtcat ggagccggta tttcctaatt
     3061 attttcaagt agaagacgag ttaggcgcat tatttcttgt actgatgact cgtgaagaat
     3121 attatagtga tcctaagata cggaagaaaa tttttgattt tcatcaacaa gcaaaaacac
     3181 cgccctttac agccctttca gaagcaaaag ccgctctttc tctttatcag gaagagcagg
     3241 ggctgccagc agaaaatcta accttcgagg cagaaaacta tttattcagc agtcattttt
     3301 tcgcctatct ttttcctaat gcgaaagaaa cgattgatgg caatagtagt gattttatta
     3361 atcatttagt tattgaaaac aaggaattga aacaatggct cgtacatttt tttgaaagta
     3421 gacacaaaca tcccaatcat ctggcattta aaaatcatgc gtttttgatg gggcggtatc
     3481 tgactgtgtt taaaacatta ggagccttta ctccacagct tccaaagatc acgatccttt
     3541 taatgacaga tttcccatta tttgaagagc aactgttaga ggagggatta cggacatttt
     3601 tccgtaatga gtatcagctg atttttctgc caacagatta tcgggggaga gaagttgatc
     3661 ttctgatttc gacaagtaaa gtccaccgca aaccatgggc ggatcttgac tattttattg
     3721 tgactgaaga actaaaatta attgattaca tccaactatc tcaaaaattt gaaatgattc
     3781 aaaaacaaaa actattaaaa caataacaaa gaaggagaaa aaagatgaca tttgaagaaa
     3841 ttttaccaga attgaaaaaa ggtgcaaaga ttattcgtaa aggatggagt ggatttgaac
     3901 tttatgttgt tctcgtatcc gatgatgaat ttgatggctg tccggtgacg ccgtacttat
     3961 tgatcaaaac aagtgacgaa ggtttttcca gcttcgctcc tacagtttgt gatattttag
     4021 cagatgattg ggcgatcgta gaatgaaaaa ccagtatcca gatctagcta aacaggttgt
     4081 atttatcaca ggagctgctt ctggaattgg tgctgcgcaa gcccgagcgt ttcttgaaca
     4141 agatgctttt gtatttggat tagatcaaaa gttcggtaaa atggaagaaa tttctttgcg
     4201 atttcctgaa acttttgcct acgcactagg tgatgttcga caaatgaaag aactccaaca
     4261 agcggtatcg aaatgccaaa atcattttgg tgaagttacg attttactga atacagcggg
     4321 gatattggat gcctacaaac ccttgatgga aactgacgaa tcgctgtggg atctgattta
     4381 tgagacgaat gtgaaaagca tgtatcagct gacaaaaatc atccttcctg atatgatccg
     4441 ccaaaaaagc ggaacgatca tcaatatggc ttcgattgct ggcttgattg ctggtggagg
     4501 tgggatcgct tatacaagcg cgaaacatgc gattgtcgga tttaccaaac aactcgctct
     4561 ggattacgca ggacagggta tccgagtaaa aggaattgct ccaggagcca tccaaacacc
     4621 aatgaatgcg gctgattttg aagggaaagg tgagatggca gagtgggtgg caaatgaaac
     4681 accagtcaag cgatgggcac agccggaaga agtagctgag ctgacgttat ttcttgcctc
     4741 tcctcaagca agctacatac aaggaacgat catcccaatc gacggcggat ggttgttaaa
     4801 ataaccttgt gtttttttca agcttctgta tgataaaact aaagagagaa acaacggttg
     4861 atgataaaaa aagtcgatga ctgttcccga cattcgatgc cgaacagcca gttcaaaaaa
     4921 ttctttttaa taatcgttaa tattcgaaac taagcggctt ttcacgacct cttgatataa
     4981 acgatattca aaagcttgca actgcggtaa caagtccagc caaatgaaaa atataaggag
     5041 ctatttttat ttgaccattc ttattacttg ttgcagggca gctttttcac taccttctgc
     5101 gacacttcag ttggaatttt cttcccatcc tttggtcgcg gtcattacca agataaaatt
     5161 atcttgcaaa ggagaaatag aacatggaaa aaaataatac ccgttcagcc aaacgctgga
     5221 caacgacaga tgtgactcgg atggcgctag tcactggttt atatgtggca gtgacgctgg
     5281 ttttgtctgt ctttagcttt ggagtgattc agattcgtct ctcggagatg ttcaactatc
     5341 tgtccttgta taacaaaaga tacatcgtcg cggtaacagc gggagtagct ttggctaata
     5401 ttgcttctcc tcttggactg atagatgttg cagtaggaag cgtcagcacg ctgcttgtat
     5461 tgcttatcaa ctataaaatc actagtcgca tcaaaaacat gaaaatcaaa atggccgtga
     5521 cagcatgcgt atttgctttt tccatgttta cagtagctgg tcagctaacg attttatatc
     5581 aactaccttt tttcttgaat tggtgggtga ttgctttagg agaattggca tcaatgattg
     5641 tcggtgggat aattatttat tggatcggta aaaaagtgga tttgtcaaaa taaagatctg
     5701 tttaatttaa aatagggcag catggaagag actgaatgtt gagtcttctt ccatgctgct
     5761 tttttgatta atttgtatga agaaatcgat ttagccgttt tttcatcaag ataccgatat
     5821 aagctgctcc agttgccttg attgcttccg gcagtaaaaa cggcagtagt ccactggcaa
     5881 atgcagttgt aaaagtaaga tcaccactta ttttcagcca gatcgttccg cagatcaatg
     5941 cagcaagaga gcccattaga ttggcaaaga ttgcatataa gtaatgagca tccgttcttt
     6001 cgatcatcca accagttatc cacgtttgta agagaaatcc aatcaaatat ccgccagttg
     6061 gcccaaataa aacagctata cctccagtca tccctgaaaa tacaggaaga ccaatcaagc
     6121 caagtaaaag ataaactcca acagatatca atcctagttt tcttccgaga aaagttgctg
     6181 ttaatccaat agcaaatgtt tgccctgtca aaggaaccaa accaaaagga atggtcatct
     6241 gagaaaggac agaaataatc gcggcaaatt ccgcagcaag tatcaattct ttcaatgttc
     6301 ggttcataac gccctctttt cgttaactta atatttttat ttggttaacg aaattgtaaa
     6361 acaaagtgat tactttgtaa atagaaaata taaaaaaaga ccagctatcc aatagatagc
     6421 ggtctttttc aagaaagctt ttgaaattat aagctttggt tgtagtattc aacgacaaga
     6481 gcttcgtcga tttctggata tagttcgtca cgttctggta aacgagtcaa tgaaccttcc
     6541 aatttttcag cgtcaaagct tacgaatgct gggcgtccaa cagttgcttc tacagcttct
     6601 tttactgtta cgatgttttg tgatttttca cgaactgaga tcacttgacc cacttctacg
     6661 tggtatgaag ggatatcaac gcgtttgcca tcaactaaaa tgtgaccatg gttaactaat
     6721 tgacgtgctt gacgacgagt cgttgcaagt cctaaacggt aaacaacatt gtctaaacgt
     6781 tgttctagta agatcatgaa gttaacacca tgtttacctt ctttgatttt gctagctttt
     6841 acgaacaagt tacggaattg acgttcgttc attccgtaca tgtgacgaag tttttgtttt
     6901 tcagttaatt gcatgccgta ttctgattgt ttgccacggc tattaggtcc atgttgacct
     6961 ggtgcgtaag ggcgacgtgc taattctttt cctgtgcctg acaatgagat gcctaagcga
     7021 cgagaaattt tccatgatgg tcctgtataa cgagacatta aaatttcctc caatgaatat
     7081 aagtttttgg agtaaaataa tccgttgaaa aattcataat catatagttt gttcttcaat
     7141 cttcaccttt gcagccgcgg ttacacaatc gaacccgaga cggggcaaca aactagggat
     7201 gagttattat ttttctgctg cattatttta cacaaattac agtatatgat agatcatcca
     7261 cgaatgtcaa gataattagt acataaaaac gctttacaaa cctaggctgt ctttataaaa
     7321 tgagagggta tgtgcaaatg gaaaaaagcg aaggaagttt aaggagaaaa tcaatgaatc
     7381 cggtatcacc attatttgag aaaatcgatg cttcaattga aaagaaggcg aatctttttg
     7441 aaaacagctt ttcacgttac gctgttcgag cggttctcgc ctgtttgttt ttgactttag
     7501 gaacggctgt ggcatttgcg atcgcgatga agggggaaga tatcgcgcat ggtctaggaa
     7561 agattttata cgcatttatg ttcagctggg gtctggtcat gattctatac atgaacgccg
     7621 aattaggaac atcgaatatg ctttatatga ccgtgggtgt ttttcggcag agacttgctt
     7681 taagtaaggc agccaagatt ctatttgcct gtattttttt caatatgatt ggcggtatat
     7741 tatgtggatt tctaatttct atgactacgc catttcaaga tttaacgttg gacaacttta
     7801 tgttagaatc aatagcagta aaactagcaa aaccttcagc aacgattttg gtggaaggga
     7861 tgtttgcgaa tatcgttgtc aataccgctg tactgatcag catgcggatg aaagaggatg
     7921 tcggaaaagt gatcacagtc gtattcatca ttttcatttt tgctttttta ggatatgaac
     7981 acgtgatagc gaattttcct gcctttacgc ttacttactt tgcttctcac ggacagatgg
     8041 atggaatgac agtcagcaat gtgttacata atttgttttt tgctttggca ggaaattata
     8101 ttggtggcgg tttagtaatg ggactgggat atgcttggct gaatcagtca aaatcatcct
     8161 atgtggacta aaaagtcgtt cattaaaggg gaagtgccat ggaagtttta gtttttccaa
     8221 aaaatgagga aaaagcgtta caaatggaaa agtacatgaa aaatcttttt tcctttgctg
     8281 gagtacctaa gccggaacgc gctcttttac aacaaccgtt actggatgag agcaaaaatt
     8341 tatctatttc tgaagtttta gcttttgttc attattatta tgaacaaatc gaaagagagt
     8401 atcagtatgt agcgatcgat ttagtccaga aaaacatcaa acggcttaca tttgaagact
     8461 tagtcgtatt gttgccacta gttcagaaaa aagaatggtg ggacagcatc gactcttgga
     8521 gaaaagtata cagcacatgg tgtaaagcct acccagagca gcgtgaagaa gtctttcaat
     8581 tgtttgaaaa aaaggaagat ttttggctac gtcgaatagc aatcacgttg caattaggtt
     8641 ataaacagca aacagacaca ggactattga tccgagcaat agacgaagat cgtcaaacaa
     8701 aggaattctt tatccaaaaa gcaattggtt gggcattacg cgattatagt aagaccgatg
     8761 ccgattgggt aaaaagacag ctgacaacag atttgagtaa gttagctgtt cgagaggcaa
     8821 gtaagtattt ataagaaagc gagacgacaa gagatgagac gcaaattatt tgcttttgat
     8881 attgatggaa cgttgttaga ttcaaacaaa caagctttag atagcacaag agaagcattg
     8941 gaaaaattac gcaaagccgg tcatctggtc acgattgcta cagggcggag ccgatttcat
     9001 gcgcaagaga tcattcgcga tcttgatttt acgaactaca ttttatgtaa tggagcggcc
     9061 ggttttttag accatgaaca agtatataag aatttactag atcgcgacca gttggatgcg
     9121 ttcgttcaag aagcgcatca tgagcagatc gatacagctt ttgtcagctt agacaatatc
     9181 aagcgttctt cttccaatga tgtaacggtg atggaagaag cgatgcattc tttcggtgct
     9241 gtcttgcctg aattagatca gttttttgca gaggaacaag aaatatacca agcgttagca
     9301 tttttcgatc aaacatatga tgggaagttc tcttcatatg ataaaattcg atttgtccgc
     9361 tggcatgaga acagtgtaga tgtcgtacct aaaaatggtt ctaaagcagc gacaattctg
     9421 cacttagcag aacaagtagg catccctcat gaagacatca tcagctttgg cgatggacaa
     9481 aacgaccgtg aaatgttgcg catgtcagga attgggatag ctatggggaa tgctgtgcca
     9541 gaagtacaag cagaagctaa gatggtcact gatacaaacg atcaggatgg aatttggaag
     9601 gcattgaaag agttaagcgt gatttaaaag aggttgtgaa aaagctgttc tgcatcaagt
     9661 aataagccca gccaaacaaa aatagctcct cctatttttc gtttttctgg gcttattacc
     9721 gcagatgcaa gctttcgaac accgtttaag gaagaggtcg tgacagaagt ggtcagtttc
     9781 aagaaataag aagggattta cgaaaattgc ttctcaaatt tttgtaaaat tacggcttat
     9841 ttctgaagaa gtagatgaaa caacgtttat tcgggaaatg ggactgtgac aagatgtttg
     9901 tcatagtccc atttttttat tttatttttc aaaactaaga aataccagga aagagttcct
     9961 tttgtttgac cttttctttc ttattgaaaa caactataat atagagtgat gttaaaagag
    10021 aagaggaatt tagtgtgcgg gtattgttta ttggtgatgt agtaggttca atgggacgag
    10081 agatgattac tgaatatctt cctagattga agaagaagta ccgtccgcaa gtaacgattg
    10141 taaatggtga gaatgccgct tctggacgtg gaatcacaga aaaaatctat aaaaaatttt
    10201 tgcaagacgg cgtagatgtc gtgacgatgg ggaatcatac gtgggacaac cgcggaatct
    10261 ttgagtttgt aaatgaagct aaaaagatgg ttcgtccggc aaattttccg gaagggacgc
    10321 ctggacaagg gatggtcttt gtcaaagtga accaagtcga actggcagtg atcaacatgc
    10381 aggctcgttc ttttatggtg gacttggatg atccttttcg taaaatgaat gagctggtag
    10441 aagaagccag aaaacgtacg ccgatcattt ttgtagattt tcatggagaa acaacaagtg
    10501 aaaaacaagc aatgggctgg tttttagatg ggaaagtttc agcagtggtc ggtacacata
    10561 cacacgttca gacaaatgat gcgcgaattt tgccaagagg aacagcgtat ctgacagacg
    10621 ttgggatgac aggtccctat gatggtattt tgggtatgcg cagagaacct gtaatcgaaa
    10681 agtttctgac tgcattacca aaacgttttg aagttgtgga acaaggccga tcgattttaa
    10741 gcggctgtat cttggaattg gatgatacga caggtcatgc aaaagaaatc cagctgattc
    10801 agatcaacga tgatcgaccg ttcatggagt agaaagagag gcagtttttt gcagcaggag
    10861 ccggaaattc aaaaagaagt aaggaaactg acaaaacttt taagagagaa tgaaacgatc
    10921 attcgctaca aagaacttga agaaaagatc cagcaaaacc agtatttagc tgagttgaga
    10981 gaaaaaatca aacaagcgca aaaagatgct gttcattttg cccactatga caaaccagca
    11041 gcagaaaaag aagcaatcaa acaagcggat caattcatgc aagaatttga tcagcatccg
    11101 cttgttgtag cttatagaaa acagctgttg gaagcagatg atctgcttca tcatttaaca
    11161 actatgatac aagaagaaat caatggacaa atagaggagg aaaaacatgc ctcaaaaaac
    11221 taaaaatact cctatgatgg aacaatatct aagcatcaag gcccaatacc aagatgcttt
    11281 tttgttctat cgtttaggag acttttatga actattttac gaagatgcga tcaaagcgtc
    11341 acagcttttg gaattaacgt tgacgagcag aaatcgaaat gcagaagaac ctattcctat
    11401 gtgcggtgta cctcaccatg cagcacaagg atatatcgat gccttgattg aaaaaggcta
    11461 taaagtagcg atttgcgagc aggtagaaga tccaaagacg actaaaggga tggtcaagcg
    11521 cgaagtgatc cagctagtca ctcctggtac agtcatggac agcaaaggat tgtcggcaaa
    11581 agacaacaat tttttaacag ctgtagttag tcaaggaaat gaatttggct ttgcttatgc
    11641 cgatttaagt acgggagaat tgaagacggc tcggttgcat gatgaagaag ctgttttaaa
    11701 tgaagcctct gcattgcaga caaaagaact cgtattaggc agtgagatca ctgataccct
    11761 tcgtgaacag ctaagcgggc gtttagggct agtcttttct caacaaaatg agatggaaga
    11821 aaacgcggaa tttagctttt tgacaagcga actagtcgat ccgctggaaa aagaagcaac
    11881 cggcaaacta ttgacttatt tatcggtgac acaaaagcgg agtttagctc atatccaaaa
    11941 agcagtagaa taccaaccag atcatttttt gaaaatggat tattattcaa aattcaacct
    12001 agaattaagc caatctattc ggactgggaa aaagcacggc actcttctat ggttgttaga
    12061 tgaaacaaaa acagcaatgg gtggccgttt actaaaacaa tggctagacc gtccgcttat
    12121 ccaggcaaaa caaattaaag caagacaaga aatggtcgct tctttgctag acagctattt
    12181 tgaacgtatc gatttgcagt cagcattaac gaaagtctat gatctggaac gcctagcagg
    12241 tcgagtagct ttcggtaatg tcaatgggcg agacttgatc cagctgaaaa cttctttaga
    12301 acaagtgcca atgatcagag ggttgattga aggaatcgat caaggtcaat ggcaaagtct
    12361 gctttcagaa atgcagccga tggaccatct cgtccaattg atcgaccaag ctatccaaga
    12421 tgatccgccg ttacaaataa cggaaggaaa catcatcaaa gatggattca atgagcagct
    12481 cgatatgtac cgttctgcta tgagaaacgg aaagaaatgg ttggcagaac ttgaagcaaa
    12541 agagcgtcaa gaaacaggaa tcaagaactt aaaaatcgga tttaaccgcg ttttcggtta
    12601 ttatatagaa attacaaaag caaatcttgg aaatgcagaa ctggagaagt atgaacgaaa
    12661 acaaacttta gcaaatgcag agcgtttcat cacaccagaa ctaaaagaat tggagacaca
    12721 aatcctcgaa gcagaagaaa aatctgttga tttagaatat cagttgtttt tagctgtgag
    12781 agaagaagtc aaaaaggcta tccagccgct acaagtactt gcaaaagcaa tcagtgcagt
    12841 agatgtgcta caaagttttg caacgatcag cgaacgctat cagtatgttc ggccagaact
    12901 tgtcagtgac aagcatcagc ttctgatcaa agacggacga catcctgtag tagaaaaagt
    12961 attaggacat caggaatata tccctaacag cgtagaaatg gcagaagacg aaatgatctt
    13021 gctgattaca ggaccgaata tgtctggtaa aagtacgtat atgcgacagt tggcattaac
    13081 tgttctgatg gcgcaaatgg gctgtttcgt tccggcaaaa caagcagttt tacctatttt
    13141 tgatcggatt tttacccgaa ttggtgcaag tgacgactta attgctggac aaagtacttt
    13201 tatggtagag atgatggaag ccaatcaagc cctgcgttat gctacgccaa acagtttgat
    13261 tttgtttgac gaactagggc gaggaacagc aacctatgac ggaatggcgt tggcccaagc
    13321 aatcatcgaa tatatccatc gtcatgtaca agcaaaaaca ctcttttcta ctcattatca
    13381 tgaactgacg gtacttgaga aagaacttcc gcagcttaaa aatgtccatg ttggagccgt
    13441 tgaaaaagac ggagaagttg tcttcttgca taaaatgatg gatggacctg cggataaaag
    13501 ctacggcata catgtggcaa agatcgcagg attgccttcc aatctactag aacgagcagc
    13561 agatatcctg catacgctag aaaatggcga taacggacac catactgttc cagctgaagt
    13621 agaagagaaa actaaaacag ccaaacagga gcaaataaaa ccacctgcag aaaaagtaaa
    13681 tcaagaccat atacaagacc gtgtaaaaga agaaaaccaa cagctttctt tattcagtga
    13741 attatccgaa aatgaaacag aagtcattga atcactgaag caaataaatt tactagaaat
    13801 gacgccaatg gatgcactaa acacattata tgaattacaa aaacaactgt aggaagaagg
    13861 cggatatcga tggccaaaat ccaagaatta tctgaacgtt tagctaatca gatcgctgcg
    13921 ggagaagtag tggagcgacc agcttccgtt gtgaaagaat tagtggaaaa tgcaattgac
    13981 gccggcagta ctcagattga tatcttagtt gaagaagcgg gactgcggac gatccaagtg
    14041 attgataacg gagaaggcat tttggcagac gacgtagaga atgccttcaa gcggcatgcg
    14101 acaagtaaga tccataatcg cgacgatctt tttcgcatac gaacgctggg ctttcgaggg
    14161 gaagctttgc caagtattgc ctctgtttct gaaatgactc tggaaacggc gaatcaggaa
    14221 gaaaaacaag gcacctatct gtcgctgaaa ggcggagaag ttattgagca tcgaccagca
    14281 ccactgcgtc aaggaacgaa gattaccgtc agcaatctgt ttttcaatac gccagcccgc
    14341 ttgaaatacg tgaaaacact gcacacagaa ttagcgaaca tcggggacat cgtcaatcga
    14401 ctggcattga gtcatccatc gattgctttt cgtttagtac atgatggaaa caaaatgctg
    14461 gcaactgctg gaaatggcga tctgaaacaa acaattgcgg gaatctacgg attatctacc
    14521 gcaaaaaaaa tgctgaaaat cgaaggaaaa gatctagatt ttgagttatt tggctatgtt
    14581 tcattgcctg aggtgactcg tgccagtagg aattacttat cgacgatcat caatggacgt
    14641 tttatcaaga acttttcctt gaataaagca atcgttgcag gttatgggtc aaaattaatg
    14701 gtcggtcgtt tccctattgc tgtattggaa atcaaaatgg acccgctttt agtagatgta
    14761 aatgttcatc cgacgaagca ggaagtccgg ctgtcgaagg aaaaagaact aacagagcta
    14821 attagtaacg ccattcaaaa agcattgtct caagaaaatc tgattccaag tgcagcggat
    14881 aatctacggt ttaagaaaaa agtacctgat cagcctaaaa cagaacaaat cgaaatcgat
    14941 ttgtcctacg cttttgaaga agaaaaacct aaaaagccaa gtagtttgaa ttttgatcga
    15001 gagacaggta cctttttcgt agaagaacta aagaacacat ttgttgacag aattgttgat
    15061 aagtctgtgg aaaatgttga aaactattct ggtttttctg aagaaaacac cacttatcca
    15121 gaaaaaacag cgaaaacggt ggataagttt ggtgaaaatg ttgataactt tgtagaaaaa
    15181 acagtggaaa aatctgaaaa gaaagaattt cccattgtgg ataacatgga gaaaacaaat
    15241 gaagaacaag cagataaatc cgtagaagag gatgaaaaga tttacgagga agaattgaca
    15301 ctccatcctg aatttgattt cactcaagga aacgacaaaa cattagaaca agccatcaag
    15361 aaattagaaa cggaaaaacc agtacaacgt tttcctcagt tggaatattt tggacagatg
    15421 cacggaacgt atctttttgc ccaaagtagc gatgggttgt acatcattga tcagcatgct
    15481 gcccaagagc gcattaagta tgaatatttc cgggaaaaaa ttggggaagt atctgatgat
    15541 ctgcaagaat tgcttgttcc atttgtgtta gactatccaa acagtgatgc tttgaaatta
    15601 aaagagcaaa aacacttgct agaagatgca gggatttatt tagaagaatt tggtcaaaac
    15661 agctttattg tacgggccca tcctacttgg tatcccaatg gagaagaaga atcgattatt
    15721 cgggagatga tcgatatgct gctgacaact ggaagtgtga gtgtaaaaaa attccgtgaa
    15781 gcaacagcaa tcatgatgag ttgcaaacga tcgattaaag ccaaccatta tttgaatgaa
    15841 gcacaagcac gtgtgctgct gaaggatctg gcacgatgcg aaaatccttt caattgtccc
    15901 catggccgtc cagtattgat tcactttacc aattcggata tggaaaggat gttcaaacga
    15961 atccaagacc cgcattaagt caaagagaag agggaaaaca tgaagattgt attagcatcc
    16021 caatcaccaa gaagaaaaga gttattggct catatcgttt cagaattcga gattgaacct
    16081 gctgatatcg atgaaacgcc atttccagac gaacagcccg tcgattatgt tcgtagaatg
    16141 gctgaagaaa aagcacgaac tgtctgggaa caagatgaaa aagaagacca tttagtgatt
    16201 gccagtgaca cgactgttgt tctcaatcaa gagatcatgg gtaaaccaga agatctggct
    16261 gaggctgaat tcatgctgaa aaaactaagc ggggaaacac atactgtata tacagctgtt
    16321 gttttaaaaa caaaggaacg agaagaacgg atactagcag aagcacatgt cactttttat
    16381 cctttgacag acgaagagat caaacgctac ttggagacgg gggattatgc ggataaggca
    16441 ggggcatacg gcatccaaaa tgctgcgagt gtattcgtca aagaaatttc tggagattat
    16501 tacagtatcg taggatttcc gattggtgct gtcaatcagg cactgaaaac attcatttaa
    16561 acaaaaaagc ggactatctg attttcgatt gaaaatccat gatagcccgt tttttttatt
    16621 gaaaagatta cttatctact ctgactaaga ttttacttca gctttaccga ctgcttcgcc
    16681 cgggcgaaca agtcctgatt ggtcaagaga aagtcctgca actttttcat tattggtcaa
    16741 agcaacaata atgtcagttt tcttgccagc ttgttctatt tctggcaaat ccatttcagc
    16801 aatcagggtc tctggtgtga caaggtatcc ttctttcacg aatacattga aaggaacacc
    16861 ctttagttca acagtatcta atcccatgtg aacaagtact tccagcccct cattagaaag
    16921 aataccaatg gcatgttttg tttcaaagac actcgtcact ttaccgttta ctggtgcata
    16981 gactttgccg tttgacggtt cgacagcata accgtcaccc atcatttttt gtgagaaaac
    17041 tgggtcattt acttcagtta tttccttgat ggtccctgca gcgactgcga ataatgtttc
    17101 ttttgttgca actgccgtac cagttgctga aacaggttcg aaagactgtg gcgttgctgt
    17161 tgcgccattt gctgtttgaa gttgtacatc agcagcattt tcaggaacag caggatcaat
    17221 cttattcaaa attccctgac gtctaaagat gatcgtcaag ccaaatggaa tgatgacagc
    17281 aatgatcatt gcgatgatga aaggtaccca tgtttctgct tggatagcta gtattcctgg
    17341 caaaccgcct actccgatcg cgttagctcg aacacccatc aagttagcaa acatgccagc
    17401 taatccagag ccgaccattg cagcgacgaa aggatagacg tatttcaaat tgattccgaa
    17461 catagcaggt tcagttacgc ctaagtaaca agaaatcatt gctggaatcg agatttgttc
    17521 ttctttttta ttcccacgat gtaagaagat gatggctaag actgcagagc cttgggcgat
    17581 atttgataaa gcaatcatcg gccataaatt cgttgatccg aaatctgcaa tcagctgcat
    17641 atcgatggca ttggtcatat ggtggagacc agtaatcact aaaggagcat acaggaatcc
    17701 gaatacggca ctaaacaacc agctgataga agacgtcaat ccagtattta cgatatttga
    17761 gatccagctt ccgactgtcc agccgatagg acctaaaata acgtgtgcag ctaaaacagt
    17821 tggtaacaat gaaaacaatg gaacaaaaat catcgagatc gactgaggga tatatttgcg
    17881 gaagaagatt tctagatacg ctaacataaa ccctgccagc atggctggaa taacttgcgc
    17941 ttgataacca atcatctgga cttgagcaaa accaaaatcc caaaatggaa tatctgctgc
    18001 ggctgtggaa gctacactat aagcatttaa taattgcgga gaaactaacg taattcctaa
    18061 aacgattcct aatatttgtg tagtacccat cttttttgca atactccaag tgatacctac
    18121 tggaagaaaa tggaaaattg cttcacccgg taaccacaaa aaggcattga caccattcca
    18181 aaacttcgag tgttcaacga ttgttccacc aagactttca aactggatac cttcaagtac
    18241 atttctgaat ccaaggatca atccaccgac aatgattgca ggaatcagtg gtgtaaagat
    18301 ttctgctaaa acggcaatag cacgttgaac gggatgaagg ttttgttttg cagctgcttt
    18361 tccttgttct ttggaaaccc cttcaactcc agatactgca acgaagtcgt tataaaaagt
    18421 agaaacatcg tttcctatga tgacttggaa ctgtcctgca ttcgtgaaca tcccttttac
    18481 gctaggtata tcttcgatcg ctttttcgtc cgcttttcct ggatcattca ggacaaaacg
    18541 catacgagtc gcacaatgac taactgcagc aatattttct tttccgccga tatcatgtaa
    18601 cagcttctcg gcatctgctt gatattttgc catatcaata aacccctttc tcttaacttg
    18661 tctatgcaag ttgtttcttc atttactata ctttttttcg gagagaattg caagcgtttt
    18721 attaaaaagt acgagagaaa atgatatcgt tttaaaaagc ttaaaataag cttttcttaa
    18781 aaataggaac attgaaatac aaaaagagct gtagtatagt gaagaaatga tggaagaacg
    18841 agtgagggaa aaaacatgaa taagttcaat gaaatttttt tagatttgga acagaaaatt
    18901 ttagcgaaag aatatcctcc gcattcgctt ttacccagtg agaaccaatt gatcaaaatt
    18961 tatggtgttt cacgagaaac aattagaaaa gcgctgaatc ttttgactac ggaaggttat
    19021 attcaaaaaa aacaaggaaa aggttcgatc gtactggatc ggaatcgctt cgactttcct
    19081 atttctggat tgactagcta taaagagcta caggagaccc aacgtattcc aagtgaaacg
    19141 atcatccata cgttagaaga aacagaagtg acaaaagcat tgaacgagat cactggctgg
    19201 gagatcggtg cgcctgtttg gcatttgata cgcgaaagaa aaatcaatgg cgaagtagtt
    19261 atattagaca ccgattatct attaaaggaa atcgttccta ttttaactcc agcacaagca
    19321 caaggatcga tctatgaata ttttgaaaat gagctttctt tgacgattgc ttacgcgcaa
    19381 aaggagatca cagtagaaga agtaacggaa atcatgaaaa cagcgatgga tcttcacgat
    19441 gacacttatg cagtagtagt cagaagcctc gttcatctgg aagatacccg ctgtttcgag
    19501 tattctgagt cggttcaccg attagataaa ttccgtttcg ttgagtttgc ccgacgccga
    19561 aaagtgtaaa tagttcaaaa gaaagcaaat tgttggcatc tgtccacttt ggcgctatga
    19621 taggatcaca aagggggcat ttttaatgga taaaccaata agaccagaaa aagaagaact
    19681 gaaagaaaag ttatcaccca tggcttatgc agtcacacaa gaaaatgcaa cagaacgacc
    19741 atttagcggg aaatatgatg atttttacga aaaggggatc tatgtagata tcgtcagcgg
    19801 tgaaccgtta ttcagctctg cagaaaaata tgatgcaggt tgtggctggc catcattcag
    19861 caaaccaata acgaaaagac aagtgcgcga aaaagccgat ttttctcatg gcatgcatcg
    19921 aatagaagtt cgaagcaaag aagcagattc tcatctcgga catgtgttta ccgatggacc
    19981 agtagatcaa ggtggtctac gttactgcat caattctgcc tcactgaaat tcattccata
    20041 tgaaaagatg gatgaattag gttatgggga attcaaatca cttgtagaat aatggaaata
    20101 cacaaagagc ggtacaatac ggggatttac tcgatcgtcc gctctttttt tattcaagcg
    20161 attgctattt gaaagaaacg ctccctagac tccttttttc acaaggtgaa atgtgctaca
    20221 ataaagggaa cgtatgtgtg caaaagaaag aggactagaa tatgtatgaa tatctgacag
    20281 gtgtggttac ctttatcaac ccctattaca tagtagtaga aaccaatggt atcggctacc
    20341 agatcgcact aggaaatcct taccgctaca gtagcaaact aaatcaaaaa ataaaaattt
    20401 atgtccatca ggtaatacgt gaagatgcgc atcttctgta tggatttgat tcgctagaag
    20461 aaaagcaact gtttttgcga ttagtaagtg tttcaggaat cggtccgaaa agtgcccttg
    20521 caatcatggc aagcgaagac cactccggtt tgattcaagc agtagaatcc agtgatgtca
    20581 cttatttgac gaaatttcca ggagtaggga aaaagacagc acaacaaatg atcttggatc
    20641 taaaaggaaa attcggtgag ctgagtatcg atacaccttt tagtctattt gatgaagcaa
    20701 acacaaaaga cgctacagct ctatctgaag caatagaagc tctctcggct cttggttaca
    20761 gtgatcgaga aatcaaacgt gtagaaaaac aattaaaaga agtagataat cagacaacgg
    20821 atgagtatct acgccaagca ctcaagctaa tgatgaaaaa ataaggagga gaccaaatga
    20881 cagatgaata cttgttatca ccagaatctg gcgaaaatga actgagtttg gaaaaatctc
    20941 ttcgaccaca gctgctttct caatatatcg gacagagaaa agtcaaacaa gaacttcaga
    21001 tttacatcga agctgccaga aatcgggaag aacctttaga tcatgttctg ttgtacgggc
    21061 ctcctggatt aggaaaaacg acgatggcca tggtcattgc taatgagatg accgtaaata
    21121 ttcgtacgac gagtggccct gctatcgaac gtccgggtga cttagtagca atcttgaatg
    21181 aactggaagc aggggatgtc ctgtttatcg atgagatcca tcgattacct agagtggtag
    21241 aagagatgct ttattcagct atggaagatt tctatgtaga tatcatggta ggacaagggc
    21301 caaccgcaca tccggttcat ttccctcttc ctccctttac tttgattggc gcgacgacac
    21361 gggcaggtat gctttctgca cctttacgag atcgatttgg catcatttct catatggaat
    21421 attatgacga acaggatctg caggagatcg ttttacgttc ttcggatatt tttcaaacag
    21481 agatcattga agaaggggca ttggaaattg ctcgccgatc acgaggaacg cctagaatcg
    21541 ctaatcgctt gctcaaacgg gttcgtgatt ttgcgcaagt ccaaggagac gggaaaatcg
    21601 atcgtcctat agcggatcag gcacttactt tgctacaggt cgatcaggct ggattggatt
    21661 atgttgacca gaaattgttg cgaacaatga tcgagttata tggcggcgga ccagtagggt
    21721 taagtacgct ttcagtaaac ataggagaag agcgtgagac ggtagaagat atgtatgaac
    21781 cgtatctgat ccaaaaagga tttttaaagc gtacacctag agggcgaatt gctactgctt
    21841 atgcctatga acattttggt tacgactatc atatatgatt tttgctttta gattggagga
    21901 aaatgaatga gtgatacaac acgtacgaaa caattgatca aaaacgtatt gattgatttg
    21961 tgccaaacgc aatcttttcg aaaaatcagc gtccaaaaaa tcgcaaacga agcaaaaatc
    22021 aatcgacaaa cattttatta tcattttaca gataaatacg atttgcttcg ttgggtctat
    22081 tgtgaggatg cattgcatta tttagatcca caatgtcttt cacttgataa ttgggaagag
    22141 caagcattga aaatgctgaa agcaattgct gagaagaaag aattttatgc aaatactgtg
    22201 ctttctgacc cagaaatttt acaaaaagag ttttctcaac tgatccaaaa atcatttatc
    22261 aagatttttg aacaaatgga tgaagaaaga caactttctg caagagacaa actattttat
    22321 gctcgttttt tttcttacgg ttgcagtggt gtattagtca attggatcac gaatgaatat
    22381 gaagaaacac cgatggaaat cgctatgcag cttttccgtt tagccaagga tacggaatta
    22441 tattcttatc gtctttatgc aaaagaagag ttggaagaac agtaatagaa aaattttttg
    22501 ttggaaaagc aataatcgct aaaaggagat ggggaaatga caaaaatttt atttgtttgt
    22561 ttgggaaata tttgccgttc accgatggca gaaggcttgc tgagaaaacg aatagcatta
    22621 gaaggaaggg aagcagaatt ttttgtggat tctgctgcta caagcacgta tgaagttgga
    22681 aagacaccgc accctggaac gaaaaaaata ttaaaccaag aaaaagtaga tatgacgaat
    22741 atggttgctc ggcaaatcac accccacgat tttgaaactt ttgattggat cattggcatg
    22801 gatcaagaaa atgtagaaga actgaaacga agagcaccac gctcagcgca ggggaaaatc
    22861 catttgtttt taagtagcat tcctggaaaa gaaaaagaaa atgttcccga cccatactat
    22921 accaataact ttgatgagac atatcacttg atcacagaag gcctgaatca ttggtggaat
    22981 atttggattg atgaaaataa aataacaaat aatttgtaaa gttaacgctt tctttttaag
    23041 gcgatgttag agcatttcct tgtttttttg taaaatgtta ggtaaaataa aattaattaa
    23101 gtaacacttt cttagctaaa ggaggagtgc attcgatgaa cgaagagaca gtatcgccaa
    23161 tgaagaagct tacgaagtta gttactcata cttcgtatca actttttgat tcttttgtaa
    23221 atgacactga agaacatgct aagaatgtac gattgcaagg aaagtttatt cttgagctgc
    23281 aaaaagctgc tactgaacat agtctagtta tcttacaagt aagagcaaat cgtttgagta
    23341 gcaagtatga aacgatctca ggctgggtgg cgacaaaaac ggtcgaaaaa gaccgagtga
    23401 tgatccgcct acaaaacgaa gagcagcaga tccggatggt tcctgtagaa caaattgaaa
    23461 aaataacaat cctttcacca gcaggggaac aggaacggtt atcaaaataa tttataccag
    23521 atagaaaagc acgtatgagc ttgtaccatc aatcattact aatcaaaaat aaccgaatgc
    23581 tttaagctga tatcagtaaa acggaacgtt ttaggatgag tatcatagca tgacagcatt
    23641 cggttgatta gttgaatcga ttggatggta caagtttttt tgataaaaaa aggtcctgct
    23701 agccggggag ctagcaggat aaggagttaa aaatgaaaaa gtgttgttag ggttgtttgt
    23761 tggtatgctt atataatagt cattagttgt gaatattttg tgacgaaagt tttcgaaagt
    23821 tgttaagttt ttgagagtat aaaatatttt gtgtaaatga aaaaatccat acaaaaaagg
    23881 aagtc
//
LOCUS       DARBET010000036        23779 bp    DNA     linear   BCT 07-NOV-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4027
            SAMEA114569841-rid20112183.denovo.036, whole genome shotgun
            sequence.
ACCESSION   DARBET010000036 DARBET010000000
VERSION     DARBET010000036.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA114569841
            Sequence Read Archive: ERR12203666
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 23779)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 23779)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001972618.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001972618.1
            Genome Coverage       :: 132x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/07/2023 13:18:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,041
            CDSs (total)                      :: 2,975
            Genes (coding)                    :: 2,908
            CDSs (with protein)               :: 2,908
            Genes (RNA)                       :: 66
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 67
            CDSs (without protein)            :: 67
            Pseudo Genes (ambiguous residues) :: 0 of 67
            Pseudo Genes (frameshifted)       :: 26 of 67
            Pseudo Genes (incomplete)         :: 28 of 67
            Pseudo Genes (internal stop)      :: 20 of 67
            Pseudo Genes (multiple problems)  :: 6 of 67
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..23779
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA114569841-rid20112183.denovo.036"
                     /isolate="VRE4027"
                     /db_xref="taxon:1352"
                     /country="Denmark"
     gene            354..2015
                     /locus_tag="SCJ86_001608"
     CDS             354..2015
                     /locus_tag="SCJ86_001608"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide ABC transporter substrate-binding
                     protein"
                     /protein_id="HEG1169149.1"
                     /translation="MKKKFTFGLVAVCGLVLAGCYGGSSATSSSSAKSGSADGGGVFN
                     LVVPQEMPTADLSVATDTISFTALNNVYEGIYRLDEDSKPQPAGASELAEVSEDGLTY
                     KIKLREDAKWSNGDPVTAADYVYGWQRTVDPATASEYAYLYAPVENAEDITAGKKDKS
                     ELGIKAVGDYELEIKLTKQTPYFQYLLAFPSFFPQKQSVVEENGDAYASASDKAVYNG
                     PFTLEDFDGPGTDTEWTYKKNDHYWDKDTVKLSEIKVSVVKESSTALNLFKDGQADDV
                     ILSGELAQQNANDPAYTSVKEARTSYIEFNQREKNSPFNNVNLRKAISYSIDREALVK
                     QVLGDGSVVSTGLIPSDMVKSPTTNEDFAKEAGKLVSYDKDKAKEYWEKAKKELGVDS
                     LEFDLMASDDDSSKKVIEYVQNSIQENLDGVTVKPTPVPFSVRLDRSTSGDFDAVLGG
                     WAADYADPSSFTDLFVTGNSYNRGRWSNAEYDEAVKAASSTDAGDEQARWDDFQKAIK
                     IISDDMGVVPVYQKAEGHLVNEKIKGIVHHAAGASWDYKWTYVEE"
     gene            complement(2085..2432)
                     /locus_tag="SCJ86_001609"
     CDS             complement(2085..2432)
                     /locus_tag="SCJ86_001609"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3899 domain-containing protein"
                     /protein_id="HEG1169150.1"
                     /translation="MKKKSIPYAVAFLLILVILIKNLINHSFTLIQLSNDLFLWSLPF
                     LIIGGFLWVFSSGFFDHFQRSVHLARTRNRKKKPEFSSLSSASYGMYSFWLIIAGILI
                     ALSAIFMLFSLLG"
     gene            2516..3457
                     /locus_tag="SCJ86_001610"
     CDS             2516..3457
                     /locus_tag="SCJ86_001610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016184893.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEG1169151.1"
                     /translation="MNSYIKYVLKRVFFMIITLWLIATITFFLMQLLPGTPYTNQERL
                     SPETIEMLNKQVGLDKPVIVQYGIYLSNLLQGDFGISFQFKNQPVAHLLAGRIGPSLQ
                     LGLQAIIFGTVLGTILGTISAMKQNTWADTSSTLVAILGRSIPNFVFAVLLQYIFAIK
                     LQVLPIAKWDGFVYTILPTIALAMSPLADSARFIRTEMVEVLHSDYVELAKAKGLSRW
                     EIAFKHGLRNSLIPLMTLLGPLAVALMTGSLVVENIFAIPGIGEQFVKSITTNDYPTI
                     MAVTILYSFMLIFVILIVDLLYGLVDPRIRVSEGSRG"
     gene            3458..4501
                     /locus_tag="SCJ86_001611"
     CDS             3458..4501
                     /locus_tag="SCJ86_001611"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345521.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEG1169152.1"
                     /translation="MEMIPKRYETVADIPADEFLPLQKNTEEEREQITAPSLNFIQDS
                     WRRLKKNKAAVISMVLLLVIILISVITIFVSPHDPTAQNVAYINLPPRIPGINIDGLN
                     GKAMVGGELVDKYAQANVPSNVNFFLGTDGLGRDVLSRLFMGMRISLLIAFIAALLDV
                     TIGVAYGLVSGLLGGRVDNVMQRFLEILSGIPNLVVMILMLVVFEPGIFSIVAAMAIT
                     NWIPMARIVRAQTMKLKDQEYVLAGLTLGESRMKIAFKHILPNISSVIIIQMMFSIPS
                     AIFFEAFLSFIGLGLTPPSASLGTMLSEGYKTFLYLPYLLWIPAVTLSVIMIGFNLLA
                     DGLRDAFDPKMKE"
     gene            4507..5577
                     /locus_tag="SCJ86_001612"
     CDS             4507..5577
                     /locus_tag="SCJ86_001612"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336675.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEG1169153.1"
                     /translation="MTKILEVKDLEISFDTYAGKVRAIRGVNFHLNKGETLAIVGESG
                     SGKSVTTRSIMRLLSSNANIDAGQILFKGNDIVGKSEREMQKIRGKEIAMIFQDPMTS
                     LDPTMPIGKQVAESLRKHNKVSKKEGQKAALELLKLVGIPEAEKRINSYPHQFSGGQR
                     QRIVIAIALVCYPEILIADEPTTALDVTIQAQILELLKEIQTKIDTSIIFITHDLGVV
                     ANVADRVAVMYGGRIVEVGTSEEIFYNPQHPYTWGLLGSMPTLDSANDRLYAIPGSPP
                     DLLNPPKGDAFYPRNEFAMKIDAEQEPPFFELSKTHKAATWLLAPQAPKVTPPEEIQR
                     RWAIFKEKQKHTYGQGGIADAK"
     gene            5567..6520
                     /locus_tag="SCJ86_001613"
     CDS             5567..6520
                     /locus_tag="SCJ86_001613"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315753.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HEG1169154.1"
                     /translation="MPNKLLEVKGLKQYFNVGKKNEVHAVNDISFHIYEGETFGLVGE
                     SGSGKSTTGRTIIRLNEPTDGEILFDGQDITKIKDKKGLTKFRHDVQMIFQDPYASLN
                     PRMKVRDIIAEGIDVNGLAKSSEERAKKVDDLLRTVGLNPSHGTRYPHEFSGGQRQRI
                     GIARALAVKPRFIICDEPISALDVSIQAQVVNLLQDLQKEHQLTYLFIAHDLSMVKHI
                     SDRIGVMHNGLLLEMGTSDEIYNHGVHPYTESLLSAIPLPDPDHERKRRRIKYQPEPD
                     DGQVRQLREIAPEHFVYATEQEVPYYAKKLKRQKESLLVAN"
     gene            6532..7542
                     /locus_tag="SCJ86_001614"
     CDS             6532..7542
                     /locus_tag="SCJ86_001614"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289328.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HEG1169155.1"
                     /translation="MLKAFKFRMYPTEEQKQQLIRTFGCARFTYNHLLKKRQKSWQQT
                     GVANFSLTPATLKKEYPFLKEVDSLALANAQLNLDRAFRNYFKGRASFPKLKTKKSMW
                     QSYTTNNQTRTIYLENGYLKLPKQKELIKINQHRPVEGSIRSATISARYNEEFYVALL
                     CDVSPVKKESLAKWIGIAYHPKTLIQTSRPLEVTLPKFHQTEEKLQHAQRKLNVKVRS
                     AHHRKIRLDQASNYQKQKRKVMDLYLKQKNQREDYLEQLSGKLVKQYDYLFVESFPKE
                     EAHADFSIHDWHKLITKLRYKSQWYNKKFLLINTDGAEESNSVRKSQVVEKMGRHSVI
                     KG"
     gene            complement(7827..8648)
                     /locus_tag="SCJ86_001615"
     CDS             complement(7827..8648)
                     /locus_tag="SCJ86_001615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853214.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX hydrolase"
                     /protein_id="HEG1169156.1"
                     /translation="MNRFASKEEEKLYYEHKASEEEFLNWYQKQERPEYDKPSLTVDI
                     VLMCYNKEEDQLKVLLIQRKGHPYRNSWALPGGFVQKDESTGESVLRETKEETGVVIS
                     KENIEQLHTFSTPNRDPRGWVVTVSYLAFIGEEPLIAGDDAKEVRWFTLERHSNKIHL
                     SSGEVAISLDLVTGESSGKDTLAFDHSQIILKAFNRVVNKMEHEPQVLQVLGKDFTIT
                     EARKVFAKFLGIDYKTIDHSNFKKAMLQYFDEIGERPVGIGRPSKIYQLKPGHHE"
     gene            complement(8745..9023)
                     /locus_tag="SCJ86_001616"
     CDS             complement(8745..9023)
                     /locus_tag="SCJ86_001616"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEG1169157.1"
                     /translation="MQKKHLFFTLSIAFLSLAHLIFSYFYIRMYGYFNLHGHLNSFMT
                     AAWILRFIIDVYIVICGFFAIREERYKVLPFYLLFFLFNLILPFIFHI"
     gene            9291..11300
                     /gene="metG"
                     /locus_tag="SCJ86_001617"
     CDS             9291..11300
                     /gene="metG"
                     /locus_tag="SCJ86_001617"
                     /EC_number="6.1.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177703.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methionine--tRNA ligase"
                     /protein_id="HEG1169158.1"
                     /translation="MAEKETFYITTPIYYPSGKLHIGNSYTTIACDAIARYKRLMGFD
                     VFYLTGVDEHGQKIETKAEELGVKPQEYVDKMAADVKKLWKTLDISYDKFIRTTDDYH
                     KAAVQKIFDRLLEQGDIYLGEYEGWYSVSDEEFFTETQLAEVYKDDEGNVIGGKAPSG
                     HEVELVKEESYFFRMSKYADRLLEYYEEHPEFIQPESRKNEMINNFIKPGLEDLAVSR
                     TTFTWGVPVKSDPKHVVYVWIDALSNYITALGYGSEDESLFEKYWPADVQMVGKEIVR
                     FHTIYWPIMLMALDLPLPKKVFGHGWLLMKDGKMSKSKGNVVYPEMLVERYGLDALRY
                     YLLRAVPFGSDGVFTPEDFVSRVNYDLANDLGNLLNRTIAMINKYCDGLVPDYASLVT
                     PFDSELSTTAANVVGRYHDAMEKMEFNTALAEIWVLISRANKYIDETEPWVLAKDEEK
                     KAELNSVMIHLAESLRIAAILLQPIMTETPEKIFNQLGLDSETMNLEGLHFGEFPSGT
                     KVVAKGTPIFPRLDMEEEVAFIQEKMSEGTQTNEDTVKWDPEETELVSTKEKQIKFDV
                     FEKVELKVAEVINCQKVEGADKLLQFRLDAGDSQDRQILSGIAEFYPDPSELIGKKVV
                     IVANLKPRKMRGQISQGMILSAEAPDGSLQVIEAPKSMPNGSEIA"
     gene            complement(11779..12366)
                     /locus_tag="SCJ86_001618"
     CDS             complement(11779..12366)
                     /locus_tag="SCJ86_001618"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345520.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEG1169159.1"
                     /translation="MDFKRSYPKLADYLSNRVDFSRKEQQQSHWHWFTLILFGLMTVA
                     TLSQKLILLTLFIAIVALIKGPVMILWGMLYSFLVGLFPPIGILLSAIFFLLNIGTFT
                     KNWRMTLVGIYFYFYPFGVMALSEVMHWDNHWFIAGSLLLGLILLHVMLTKLYQHYGI
                     GRTIFWYVFSIPFALLTALLPSRLKTKIKGYHKIK"
     gene            12541..13863
                     /locus_tag="SCJ86_001619"
     CDS             12541..13863
                     /locus_tag="SCJ86_001619"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HEG1169160.1"
                     /translation="MKGKLKREINLFGALATVMGTVIGAGVFFKTAAVTASTQSTSLT
                     LLAWLLGGFLTICAGLTVAELATAIPETGGAVKYIEAAYGKLPSFLLGWAQSLIYFPA
                     NIAALSIIFATQLTNLLQLSTDYLLLIAVITAVSVTGLNLLGTKVGASVQSVTLIVKL
                     IPIAVIVIWGLLTPGQGTVQLFPIEAGKDVTFVEGLSSALLATLFAYDGWLGVGAMAG
                     EMKRPEKDLPKAIILGLSFVTVVYLLINFVFLKTLPIDHLAGNLNAASEASDVIFGGI
                     GGKLVTIGILISVYGALNGYTLTGIRVPYAMALEDELPFSKQLTKLSKKFTVPYVPAV
                     FQLAVACIMMSLGSFDFLTDMLIFVMWLFSLLIFIGVFILRKKAPELPRPYKVPLYPI
                     VPIIAILGAIFILGMTMMTQTKLALIGIGVTLIGIPVYYQKKKKRSEE"
     gene            14018..14794
                     /locus_tag="SCJ86_001620"
     CDS             14018..14794
                     /locus_tag="SCJ86_001620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315759.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TatD family hydrolase"
                     /protein_id="HEG1169161.1"
                     /translation="MIFDSHTHLNAEQFNEDIPETIQRAQEMGVTKMAVVGFDTPTIE
                     KSLQLSHDYPNIYSIIGWHPTEAGSYTKEIENKLQEQLTLPKVVALGEIGLDYHWMED
                     PKEVQDKVFRRQIAIAKEMNLPISIHTREALEDTYQILKEEDVRDIGGIMHSFSGDYE
                     WAKRFLDLGMHISFSGVVTFKKALDVQEAAANVPIERLLVETDAPYLAPVPFRGKRNE
                     PGYTRYTVEKIAELRNLPTEEVAAQTWTNAHRLFGLSEHG"
     gene            14787..15356
                     /gene="rnmV"
                     /locus_tag="SCJ86_001621"
     CDS             14787..15356
                     /gene="rnmV"
                     /locus_tag="SCJ86_001621"
                     /EC_number="3.1.26.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310144.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease M5"
                     /protein_id="HEG1169162.1"
                     /translation="MDKMKIQEIIVVEGKDDTRRLREVVDADTIETIGSAINEEILMQ
                     IEHAQETRGVIVFTDPDFSGEKIRKIIMEAVPQAKHAFLPRTQAKPKKKGSLGVEHAS
                     DAAILEALKKVVTPDISQTAVSEITRQDLLDYGLIAGAQAKEKREKLGDELRIGYTNG
                     KQLEKRLKMFRITPKELAQAMKIVEEQHE"
     gene            15349..16233
                     /gene="rsmA"
                     /locus_tag="SCJ86_001622"
     CDS             15349..16233
                     /gene="rsmA"
                     /locus_tag="SCJ86_001622"
                     /EC_number="2.1.1.182"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294004.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA
                     (adenine(1518)-N(6)/adenine(1519)-N(6))-
                     dimethyltransferase RsmA"
                     /protein_id="HEG1169163.1"
                     /translation="MSEYREIATPSRTKEILLKHGFSFKKSLGQNFLTEPNILRKIVE
                     TAQIDEHTNVIEVGPGIGALTEQLAKHAKQVVAFEIDDRLIPVLADTMQPYDNVTIVH
                     QDILKTDLSTAVRETFHEELPLKVVANLPYYITTPIMMHFLESDLVVDELVVMMQKEV
                     ADRISAEPGTKAYGSLSIAVQYYMEASLAFIVPKTVFVPQPNVDSAILKLTRRDIPAV
                     EVTDEKAFFRLTKAAFQQRRKTLWNNLQHSYGKDDQTKAWLAKSLETAGIDPKRRGET
                     LSLQEFAALSNAMSENMQ"
     gene            16684..17313
                     /locus_tag="SCJ86_001623"
     CDS             16684..17313
                     /locus_tag="SCJ86_001623"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289506.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEG1169164.1"
                     /translation="MRKNSVRKLTISALLIGMGVIIPMVMPKIMIPPASFTLASHVPL
                     FIAMFFTPGVAVAVALGTTFGFFLTTPVIIALRALSHLVFALIGAWYLQKHPSIVLKN
                     GQFTFANWRFQGFNFVIGVIHSLAEMLVVSIFYFIGNMPETYYSQGYFYTVFILMGIG
                     GLIHSLVDYNIAYFIAGTLSKYFDIPVFTAAKKENRKESIQGYAKKTTY"
     gene            complement(17437..18546)
                     /gene="ugpC"
                     /locus_tag="SCJ86_001624"
     CDS             complement(17437..18546)
                     /gene="ugpC"
                     /locus_tag="SCJ86_001624"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016625778.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sn-glycerol-3-phosphate ABC transporter
                     ATP-binding protein UgpC"
                     /protein_id="HEG1169165.1"
                     /translation="MVEMALKNVYKKYDNAENYSVTDFNLSIKDREFIVFVGPSGCGK
                     STTLRMIAGLEDISEGELNIGGKVMNDVAPKDRDIAMVFQNYALYPHMTVFDNMAFGL
                     KLRKYDKADIKKRVDNAAEILGLTEYLKRKPAALSGGQRQRVALGRAIVRDAKVFLMD
                     EPLSNLDAKLRVAMRAEIAKLHQRLETTTIYVTHDQTEAMTMADRIVIMKDGFIQQIG
                     TPKEVYDTPVNMFVAGFIGSPAMNFFNVTLKNGVITDGHGLKLRIPEGKNKVLVEKGY
                     EGKEVIFGIRPEDIHSEQVALEASPEAVVKSEVVVSELLGAETMLYTKTGSTEFISKV
                     DARDFHKPGEIVDLAFNISKGHFFDKETEQVIKLP"
     gene            18755..19177
                     /gene="mgsA"
                     /locus_tag="SCJ86_001625"
     CDS             18755..19177
                     /gene="mgsA"
                     /locus_tag="SCJ86_001625"
                     /EC_number="4.2.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330705.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methylglyoxal synthase"
                     /protein_id="HEG1169166.1"
                     /translation="MKIALIAHDRKKPMMVKLATAYKTILEKHELFATGTTGQRITEA
                     TGLPIHRFKSGPLGGDQQIGAMISEDQLDLVIFLRDPLAAQPHEPDVTALIRLSDVYE
                     IPLATNIGTAEVLLRGLEAGFLDFRRIVHEIDKHPLSF"
     gene            19500..20036
                     /locus_tag="SCJ86_001626"
     CDS             19500..20036
                     /locus_tag="SCJ86_001626"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330706.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="folate family ECF transporter S component"
                     /protein_id="HEG1169167.1"
                     /translation="MKKHRLDARMIAIMGLLIALMVTLSRLVAIETPFIKISVTFIPQ
                     VIMGILFGPFWSGIGAVLADLVGMALFSKSAFFIGFTLNAFIEGAIYGFFFYRKEITW
                     KNAILATLSVTLIINLFLTPLWLALMYHVPLFSWVVWAPRLLKTVIWLPIQSIAIYYV
                     GRSIPYKKILRSLAIHAK"
     gene            complement(20076..21839)
                     /locus_tag="SCJ86_001627"
     CDS             complement(20076..21839)
                     /locus_tag="SCJ86_001627"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289511.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEG1169168.1"
                     /translation="MKVKKLSLQTLRRFIPYLTAYAKDIWLAVILGIINGIATVVMTL
                     QIGQSIDQMIGEGQVRFNRLFHLLILFGGIVLINVVSQWLIQVLSNRLAYLSVAKLRK
                     DAFAHLNRLPLRYYDQNSHGNIVSRFTNDMDNISIAISAAFNQLFSGVAIIILSFIFM
                     MRLSPLLTLVVLCSTPIIFLVSWIVARASQTNFDRQQRIIGDISGFVTERIGNQKIVK
                     AFQQEEASQQQFELLNADLYEKGQRAQFSSSLTNPSSRFVDHLAYLSIGLVGGLLALK
                     SGSSITVGVISSFTIYSSQFSKPFIELSGITTQLQTAFSGLERAFDLIDQPEEKPDLP
                     DAYALEPKNIKGKVSFDHVSFSYDPKQRLIEDFNFTAYPGQTIAIVGKTGAGKSTLVN
                     LLMRFYDTTKGTITVDDHDIRSIQRDTLRKSFGMVLQDTWLFDSTLRENLRFGNPEAS
                     DAEINDALKASYMYEFVERLPEKLDTKIGGQGLKISDGQRQLLTIARTMISNPPMLIL
                     DEATSSVDTLTEKKIQQAFLAMMEGKTSFVIAHRLSTIKNADQILVMDQGAIVEMGTH
                     DELLQKDGYYRRLYQAQFTAQ"
     gene            complement(21842..23557)
                     /locus_tag="SCJ86_001628"
     CDS             complement(21842..23557)
                     /locus_tag="SCJ86_001628"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEG1169169.1"
                     /translation="MIQLLRYAKDYRKQIILGPFFKFLEAVFELVLPLMMASLIDNGL
                     KMNDRGKIIEMGLWMVAMSVIGLICAIICQYYASVASQGFGTELRNQLIKKINTFSHN
                     ELNHFGTDTLITRMTNDINQLQLALAMVIRLLIRAPFLSIGSVVMAFVIDWEVGLFFL
                     ALLPIFSIILFFIIKKTVPLYQKVQEKLDQLNEHVSQNLSGVRVIRAFAQTKKETEKF
                     DKSTNDLANNYLRVSNISALLSPATTLIMNVGIICLLTVGGIKVNIGSLQQGQVLALI
                     NYMNQMLLALIVVSNLVVIFTRAEASGNRVKEVLDTENSILEAEVSTTPDFSSEAIIA
                     FDHVDFRYTPDSGLSLQNITFQLKRNTVLGIIGPTGSGKTTLTQLIPRFYDVSSGTVI
                     MDGMDVRSWQLDHLRQQIALVPQTSVLFTGTIRENLQWGKKDATEKECWEALKIAQAE
                     EFVRQLPNGLDTKVMENGKNFSGGQKQRLTIARALIAKPALLILDDSLSALDYQTDLD
                     LRKALQTYLGSTVIIISQRVRSIQEAHHILVMDQGKIAAQGTHEELLAQSAEYQEIVA
                     SQEEE"
BASE COUNT         7667 a         4341 c         4818 g         6953 t
ORIGIN      
        1 atttttgcat aaatttggct taataccgag aaactggctt ttgaacaccg tttattcaga
       61 taagggactg tgacaagact tttgtcacag tttctttttg tttattcttt tttatctttg
      121 tatggcaata atagacaata taacgaaaat tatttaatgg cttttggaag accatggaaa
      181 accttgtaca aagctatttt ttctgaaaaa ttccttgaaa cctcacgaaa aaatcattat
      241 tttatgaaaa agacaaaaaa aagtgttgat tttttaataa ttattcatta aactcttaaa
      301 caacttcaga aaatattaat tattctgaaa attccaataa aggggtacgt aaaatgaaaa
      361 agaaatttac atttggtttg gttgcggtat gcggccttgt acttgctgga tgttatggcg
      421 gcagttcagc cacaagcagt agttcagcaa aaagcggttc agcagatgga ggaggcgtat
      481 ttaacttagt cgtgcctcaa gaaatgccta cagctgactt atcagttgca acagatacaa
      541 tcagttttac agcattgaat aacgtttatg aaggaattta ccgtcttgat gaagacagca
      601 aaccacagcc ggcaggagct agcgagttgg cagaagtcag tgaagacgga ttaacttaca
      661 aaatcaaatt acgagaagac gcaaaatggt caaatggtga tcctgttaca gcagcggatt
      721 acgtttatgg ctggcaacga acagtagacc cagctacagc ttctgaatat gcttacctgt
      781 atgcgccagt tgaaaatgca gaagacatta cagctggaaa gaaagataaa tcagaactag
      841 ggatcaaagc agttggtgac tatgaattag aaatcaaatt gacgaaacaa acaccatatt
      901 tccaatatct gcttgctttc ccatcatttt tcccacaaaa acaatccgta gtggaagaaa
      961 atggggatgc ctatgcatct gcaagtgata aagctgttta taatggacca ttcacattag
     1021 aagattttga tggaccagga acagatacag aatggactta taagaaaaat gatcattact
     1081 gggacaaaga tacagtaaaa ctttctgaaa tcaaagtcag tgtagtaaaa gaatcttcta
     1141 ctgcattgaa cttattcaaa gatggccaag cagatgacgt gatcttgtct ggtgagttag
     1201 cacagcaaaa cgcaaacgat ccagcttata cttctgttaa agaagcgcgg acaagctata
     1261 tcgaattcaa tcaacgtgaa aagaattcac cattcaacaa tgtgaaccta agaaaagcta
     1321 tttcttattc tatcgatcgt gaagctttag taaaacaagt actaggtgac ggatcagttg
     1381 tctctacagg attgatccca tcagatatgg taaaaagtcc aacaacgaac gaagactttg
     1441 caaaagaagc tggaaaatta gtttcttatg acaaagataa agcaaaagaa tattgggaaa
     1501 aagcgaaaaa agaattgggc gttgattctt tagaatttga tttgatggct tctgatgatg
     1561 attcatcgaa aaaagtaatc gaatatgttc aaaactcaat ccaagaaaac ttggatggcg
     1621 taacagtcaa accaacacct gttccattct ctgtacgtct tgaccgctca acttcgggtg
     1681 attttgacgc agtactaggc ggatgggctg ctgactatgc agacccaagc agctttactg
     1741 atttgtttgt cacaggtaat tcatacaacc gtggtcgctg gagcaatgca gaatatgacg
     1801 aagcagttaa agcagcttca tcaacagacg ctggcgatga acaagcccgt tgggatgatt
     1861 tccaaaaagc aatcaagatc atctctgatg atatgggtgt cgttccggtg taccaaaaag
     1921 cagaaggtca cctagtgaat gaaaaaatca aaggtatcgt tcaccatgcg gcaggcgctt
     1981 catgggatta caaatggaca tacgttgaag aataatcgtt aataccgtga gcatgagaaa
     2041 aaacgaggct gcggcaaatg tcgcaacctc gttctcatat tcacttatcc taacaaagaa
     2101 aaaagcatga aaatagcaga aagagcaatt agtattccgg caatgatcag ccaaaagctg
     2161 tacataccgt agctggcaga cgagagcgag gaaaattctg gttttttctt tcggttcctt
     2221 gttcgtgcaa gatggaccga tcgctgaaaa tgatcgaaga aacccgaaga aaagacccat
     2281 aaaaaaccgc cgataatcag aaacggcaat gaccataaga atagatcatt agataattga
     2341 atgagtgtaa aggaatggtt gatgagattt tttattaaaa taaccagaat cagtagaaaa
     2401 gctactgcgt aggggataga tttttttttc atttgattgc tcctttattt ttggttcatt
     2461 atctaataat gtagcgaaac atctgtcaga agtcaaaagc tagggggaaa cattagtgaa
     2521 tagttatatc aaatatgtat taaaacgtgt ctttttcatg atcatcacgc tttggttaat
     2581 tgcaacaatc acctttttct tgatgcaatt gctgccgggc acgccatata cgaatcagga
     2641 acgtttgagt cctgaaacaa tcgagatgct aaataaacaa gtcggtttgg ataaaccagt
     2701 tatcgtacaa tatgggattt atctttctaa tttgcttcaa ggagattttg gaatttcctt
     2761 ccaatttaaa aaccagccag tcgcacattt gttagctgga agaatcggac catccttgca
     2821 gttagggtta caggcaatta tctttggaac agtcctaggg acgattttag gaacgatctc
     2881 tgccatgaag caaaatacat gggcagatac atcttctaca ctcgtagcga tattaggtcg
     2941 ttccattcca aactttgtat ttgctgtttt attacaatat atctttgcaa tcaaattaca
     3001 agttttgcca atcgcaaaat gggatggctt tgtgtacaca atcttaccaa caattgcgtt
     3061 ggccatgtcg ccgcttgctg actctgctcg gtttatccgt acggaaatgg tggaagtact
     3121 gcatagtgat tatgtggaat tagcaaaagc aaaaggattg agccgttggg aaatcgcctt
     3181 taaacatggt cttcgaaata gtttgattcc attgatgact ttattaggac cgttagctgt
     3241 agcattgatg accggatcac tggtggtaga aaatatcttt gctattcctg gtatcgggga
     3301 acaattcgtt aagtcaatca caacaaatga ttatcctacg atcatggctg taacgatcct
     3361 ttactcattt atgctgattt ttgttatttt gatcgtggat ctattgtacg gtctagttga
     3421 tccaagaatt cgtgtttcag aagggagccg aggataaatg gaaatgatac caaagcgtta
     3481 tgaaacagtt gcagatatcc ctgcggatga atttcttcca ttacaaaaaa ataccgaaga
     3541 agaacgtgaa cagattactg caccttcttt gaattttatc caagattcat ggcgccgttt
     3601 aaagaaaaac aaagctgccg ttatttcaat ggttttacta ttggtcatca ttttgatctc
     3661 tgtgatcaca atctttgttt ctcctcatga tccaactgcc caaaacgtgg cttatatcaa
     3721 tttgcctcct cgtattccgg gaatcaatat cgatggattg aatggaaaag caatggttgg
     3781 tggtgaactt gttgataaat atgctcaagc aaacgtccct tcaaacgtaa atttcttttt
     3841 aggaacagac ggtttgggac gtgatgtatt aagccgctta tttatgggaa tgagaatctc
     3901 gttgctgatc gcatttattg ctgcattact agatgtaacg attggtgtag cttacgggtt
     3961 ggtttctggt ttgttaggcg gacgtgtcga taacgtgatg caacgttttt tggaaatctt
     4021 atcaggaatt ccaaacctcg ttgtcatgat tttgatgttg gtcgtttttg aaccaggaat
     4081 tttttcaatc gtggctgcta tggccatcac aaactggata ccaatggcac gtatcgttcg
     4141 tgcacaaaca atgaaattaa aagatcaaga atatgtttta gctggattga cattaggtga
     4201 atcaagaatg aaaattgcat tcaaacatat tttgccgaat atctctagtg tcattattat
     4261 tcaaatgatg tttagtattc catctgctat tttcttcgaa gcctttttaa gctttatcgg
     4321 tttaggtctt acgcctccgt cagcttcatt aggaacgatg ttgagcgaag ggtacaagac
     4381 attcctgtat ctgccttatc tattatggat tccggcagta actttatcgg ttatcatgat
     4441 tggctttaac ttacttgctg atggattgcg tgatgccttt gatcctaaaa tgaaagagtg
     4501 aagacgatga cgaaaatttt agaagtgaaa gacttagaaa tctcttttga tacgtatgct
     4561 ggaaaggtac gagcgattcg cggtgtcaat tttcatttga ataaaggaga aacattggcg
     4621 atcgttgggg aatctggaag tggaaaatca gtgacgacca gaagcatcat gcgcttgctg
     4681 tcaagcaacg ccaatataga tgctggtcaa atcctcttta aaggtaacga tatcgttgga
     4741 aaatcagaaa gagagatgca aaaaatccgt ggtaaagaaa tagcgatgat tttccaagat
     4801 ccaatgacct ctttagatcc gacgatgccc attgggaagc aagtcgctga gtcccttaga
     4861 aagcataata aagtttcaaa aaaagaggga caaaaagctg cattagaatt gcttaaatta
     4921 gttggaatac ccgaagctga gaaacgtatc aacagttatc cgcatcagtt ctcaggaggt
     4981 cagcgtcaac ggatcgtgat cgcgattgca ctggtatgtt atccggaaat cctgattgct
     5041 gatgaaccta caacagcgct tgatgtgact attcaagcac agattttgga gctgctgaaa
     5101 gaaattcaga caaaaatcga tacatcaatt atctttatca ctcatgacct tggtgtagta
     5161 gcgaatgtcg ctgatcgtgt cgctgtcatg tatggaggaa gaatcgttga agttggaaca
     5221 tcggaagaaa ttttctacaa cccgcaacat ccctatactt ggggactgct tggttcaatg
     5281 ccgacattag atagtgcgaa cgatcgattg tatgctattc ctggttctcc accggattta
     5341 ttgaacccgc caaaaggaga cgcattctat ccaagaaatg aattcgcgat gaaaatcgat
     5401 gcagaacaag aaccaccatt ttttgaacta tcaaaaacac acaaggcagc aacttggcta
     5461 cttgctccgc aggcacccaa agtgacaccg ccagaagaaa tccaacgtcg ctgggctatc
     5521 tttaaagaga aacaaaaaca tacttacggt caaggaggaa ttgctgatgc caaataaact
     5581 acttgaagtt aaaggattga aacagtattt caatgtagga aaaaagaatg aagtacatgc
     5641 tgtaaatgac atcagctttc acatttatga aggagaaaca ttcggtttag ttggagaatc
     5701 tggaagcgga aaatccacta ctggccgtac gatcattcgc ttgaatgaac ctactgatgg
     5761 tgagatatta ttcgacggtc aagatataac gaagatcaaa gataaaaaag gtttgacgaa
     5821 attccggcat gatgttcaga tgatctttca agatccatac gcttcgttga atcctcgtat
     5881 gaaagtcaga gatatcattg ccgaaggaat tgatgttaat ggactagcta aatcatctga
     5941 agagcgtgca aaaaaagtcg atgatctatt gagaactgtt ggattgaatc caagccacgg
     6001 tactcgttat cctcatgaat tttcaggtgg gcaacgccag cgtattggta ttgcacgagc
     6061 attggcagta aaaccgcgtt ttatcatttg tgatgaacca atttctgcac tagatgtgtc
     6121 gatccaagcc caagtggtca atttgttgca agatctccaa aaagaacatc aattgaccta
     6181 tctatttatt gcacatgatt tgtcgatggt taaacatatt agtgaccgaa tcggtgtaat
     6241 gcataacggc ttgttattag agatgggaac aagtgatgaa atctataatc acggtgttca
     6301 tccttataca gaaagtttgt tatctgctat tcctctacca gatccagacc atgagagaaa
     6361 acgcagaaga atcaaatatc aacctgaacc agatgacgga caagtacgtc aacttcgtga
     6421 aatcgcacca gaacattttg tctatgcaac tgagcaggaa gtcccatatt acgcaaaaaa
     6481 actaaaacga caaaaggaat ctcttctggt tgctaactag gagggacata gatgctaaaa
     6541 gcttttaaat tccgtatgta tccgacagaa gaacaaaaac agcaattgat tcgtactttt
     6601 ggatgtgctc gatttaccta taaccatttg ctgaaaaagc ggcagaaaag ttggcaacaa
     6661 acaggagtag ccaatttttc gctcacaccc gctacattaa aaaaagaata cccattctta
     6721 aaagaagtag acagtcttgc tttagcaaat gcacagctta atttggatcg ggcttttcgc
     6781 aattatttca agggacgtgc cagcttccca aagcttaaaa cgaaaaaaag tatgtggcaa
     6841 tcttatacga ccaacaacca aacccgtacg atctatctgg aaaatggcta cttaaagtta
     6901 ccaaaacaaa aagaactaat caagataaat cagcatcggc cagttgaagg atcgattcgc
     6961 tccgctacga tttctgctcg atataatgaa gaattttatg tcgcactact ttgtgatgtt
     7021 tcgccagtaa aaaaagaatc tttagctaaa tggatcggga tcgcctatca tccgaagaca
     7081 ttgatccaaa catctcgacc tctagaagta actttaccaa aatttcacca gacagaagaa
     7141 aagctgcaac atgcacagcg gaaattgaat gtgaaagtcc gctccgctca ccatagaaag
     7201 atcaggttag atcaggcaag caattatcag aaacaaaaga gaaaagtaat ggatctttat
     7261 ctgaaacaaa agaaccagcg agaagattat cttgaacaat tatctgggaa attggtcaaa
     7321 caatatgact acttatttgt agaatctttt ccaaaagaag aggcacatgc tgatttttct
     7381 attcacgact ggcataaatt aataacaaaa ttgcgttata agtcccaatg gtataataaa
     7441 aaatttcttt tgatcaatac cgacggagca gaagaaagca attccgtaag aaagagccaa
     7501 gtagtagaaa aaatgggcag acattcggtt atcaagggat aaaaaatgaa tgaaactgat
     7561 aggagcgtta gggttagcct tgttaataaa aagtcttaat gacttttgtt tctaggaatt
     7621 tttcttttga agaaaagctc actaattatg acaaaccaat aaaacaatag cagaaaaagt
     7681 tcgtacgagc tttttctgct attgttaatt ttttattcag aaagtttacg aatacatcaa
     7741 aattctgaaa caaaaaacgt aactcacaaa gagttgtttt tgcgtttttt cttgaatgat
     7801 taggtctgaa tcttttgctc acaaccttat tcatggtgcc ccggcttgag ctgatagatt
     7861 tttgaaggac ggccgattcc tacaggacgt tcaccgatct catcgaaata ttggagcata
     7921 gctttcttaa aattggaatg atcgatcgtt ttataatcga tccctaagaa tttagcaaat
     7981 acttttcgag cttccgtaat cgtgaaatct ttacctaaaa cttgtagaac ttgcggctcg
     8041 tgttccattt tattgactac acggttgaat gctttcaaga tgatttgact atggtcaaaa
     8101 gccaaagtat ctttgccaga agactctcca gtgactaaat caagcgagat agccacttct
     8161 ccgctagata aatggatctt gttactatga cgttctagtg tgaaccagcg tacttccttg
     8221 gcatcatctc cggcgattag cggttcttct ccaataaagg ctaagtagct gactgtgacg
     8281 acccagcctc gcggatctcg attaggcgta ctgaatgtat gcagttgctc aatattttct
     8341 tttgaaataa ccacaccagt ttcttccttt gtttctctta agacgctttc tccagttgac
     8401 tcatcctttt ggacaaagcc accaggcaat gcccatgaat ttcgataagg atgccctttt
     8461 ctttggatca acaaaacttt cagctgatct tcttctttgt tatagcacat cagtacgata
     8521 tcgacagtca aagagggctt gtcatattct ggacgttcct gtttttgata ccagttaaga
     8581 aattcttctt cacttgcttt atgttcataa tataattttt cttcttcttt agaagcaaaa
     8641 cggttcatct gaccacctcc attgacagta aaagtacttt ttaccttatt gtagcagact
     8701 ctgaaaaaaa accaaggatt tacttgaaaa atctaggtta tcatttaaat atgaaaaata
     8761 aatggaagta tgagattgaa taaaaagaaa agcagataaa acggcaatac tttataccgc
     8821 tcttcacgaa tggcaaagaa gccacaaata acgatatata cgtcaatgat aaaacgcaaa
     8881 atccaagctg ctgtcataaa actgttcaaa tgcccatgga ggttaaaata accatacata
     8941 cgaatataaa aatagctgaa tattaaatgt gctaatgaaa gaaaagcaat actaagtgta
     9001 aaaaaaagat gtttcttctg catgattgtt ccagtccctt actaagatag atagtcttta
     9061 ttataccacg tgtgagggga gaaataaaaa gccgtttttt agtagatatg aaacaaaaca
     9121 aaaacccgca tttattcaac ctattcatcc ttttaaagga tagattgaac gtagatagaa
     9181 gtcgtttatc cagtcttttt cattttgaaa aaaagctaac agcatgcttt ttttatggta
     9241 tactagcaag gaaacgaatt cgcatcatgc gataaaggag gaagagcccc gtggcagaaa
     9301 aggaaacttt ttatatcact acgccaatct attatccaag cggcaaactt catatcggca
     9361 actcgtatac tacgattgca tgtgacgcaa ttgctcgtta taaacggttg atgggctttg
     9421 atgtgtttta tctcactgga gtggatgaac atggtcaaaa aatcgaaaca aaagcagaag
     9481 aactaggtgt aaaaccgcaa gaatatgtag ataaaatggc ggctgatgtc aagaaattat
     9541 ggaaaacgct tgatatcagt tatgataaat tcattcgcac gacagatgat taccataaag
     9601 cagctgttca aaaaatattt gatcgtttat tagaacaggg agatatttac ttaggtgaat
     9661 atgaaggctg gtactctgtt tctgacgaag aattctttac agaaacacaa cttgcagaag
     9721 tgtacaaaga tgacgaaggt aacgtgatcg gtggtaaagc gccaagtgga cacgaagtag
     9781 aattggtaaa agaagaatca tacttcttcc gcatgagcaa atacgcagac cgtctattgg
     9841 aatactatga agaacatccc gaattcatcc agccagaatc tcgtaaaaac gaaatgatca
     9901 acaactttat caaaccagga ttagaggatc ttgcagtttc acgaacaact tttacttggg
     9961 gagtaccagt caagagtgat ccgaaacatg tggtgtatgt atggatcgat gcattatcca
    10021 attatattac tgcactagga tatggctccg aagatgaaag tctattcgaa aaatactggc
    10081 ctgcagatgt tcagatggtc ggtaaagaaa tcgttcgctt tcatacgatc tattggccaa
    10141 tcatgctgat ggcccttgat ttgcctctac ctaaaaaagt atttgggcat ggctggctat
    10201 tgatgaaaga cggaaaaatg tctaaatcaa aaggaaatgt cgtttatcct gaaatgcttg
    10261 tggaacgtta tggtttagat gcactccgtt attatttgct tcgtgctgtt ccgtttgggt
    10321 cagatggtgt ctttacgcca gaagattttg tttcccgtgt caattatgat ctggcaaatg
    10381 atctagggaa cttattgaat cgaacgattg ctatgatcaa caaatattgt gatggactcg
    10441 ttcctgacta tgcctcactt gtcactccat ttgatagtga actgtctacg acggctgcta
    10501 acgttgtcgg acgttaccat gatgcaatgg aaaaaatgga attcaataca gcattggctg
    10561 aaatctgggt gctgatttct cgtgcaaaca aatacattga tgaaacagaa ccatgggtat
    10621 tagcaaaaga cgaagaaaag aaagctgaat tgaatagtgt catgatccat ttagctgaat
    10681 ctttgcgtat cgcagcgata ttacttcagc caatcatgac agaaacgcca gagaagatct
    10741 tcaatcaact aggattggac tctgaaacaa tgaatcttga aggattacac tttggcgaat
    10801 ttccttcagg aacaaaagta gttgcaaaag gtacaccaat cttcccacgt ttagatatgg
    10861 aagaagaagt tgcttttatt caagaaaaaa tgtctgaagg tactcaaaca aacgaagata
    10921 cagtcaaatg ggatccagaa gaaacagaac ttgtttcaac gaaagaaaaa caaatcaaat
    10981 ttgatgtatt tgaaaaagta gaactaaaag tagcagaagt catcaactgt caaaaagtag
    11041 aaggggcaga taagttgctg caattccgac tagatgctgg agacagccaa gaccgtcaaa
    11101 tcttgtcagg aatcgctgaa ttttatcctg atccaagcga attgatcggg aaaaaagtcg
    11161 tcattgtagc aaatctgaaa ccacgaaaga tgcgtggaca aatcagtcaa ggaatgatcc
    11221 tttctgcaga agcaccagat ggctctttac aagtaatcga agcaccaaaa tccatgccaa
    11281 atggctcaga aatcgcatag aaattcaaag ggggtatgac actagttgtg tcataccccc
    11341 tttttccgaa atttgagaag caattttcgt aaattccttc ttattacttg aagccaatca
    11401 tataatacag aaacagagaa ctatccaaaa taaatcctag aaaaagaagt acgagcttcg
    11461 ctgatttttg ttaatttaga ataatactaa taggaatcgt aaaaacagaa aaaaagcata
    11521 atgataaagg gtcgggcaga ttttaattga ttcatctggt actctttttt attgtcctta
    11581 cattcataaa gtttgaatgc aatgacttta ttacatctat atttatatct aacagtagcg
    11641 ttgatgtgtt aattataagt tctatcactt tatgttcaat tagtttgtgc tcttatatgt
    11701 gtgattgaaa atatagataa aaaaacaatc atcggctttg acttcgatga ttgttttctt
    11761 tattttgtaa aaatcctatc acttgatttt atggtaacct tttatttttg tcttcaaacg
    11821 gcttggtaag agagcagtaa gcagtgcaaa tgggatcgaa aagacatacc agaaaatcgt
    11881 acgtccgata ccataatgtt gatagagctt tgtcaacatc acgtgtaaca agatcaaccc
    11941 taaaagcaag gagccagcta taaaccaatg attatcccaa tgcatgactt cactaagtgc
    12001 catcactcca aaaggataga aataaaaata gatcccgact aaggtcatcc gccagttctt
    12061 tgtaaatgtt ccgatattca ataaaaagaa aatcgctgaa agcaaaatac cgataggcgg
    12121 aaaaagccca accaaaaagg aatacaacat cccccacaag atcatcacag gacctttgat
    12181 caatgcaacg attgcgatga acaaagtcag caatattagt ttttgtgata acgttgcaac
    12241 agtcattaat ccaaataaaa ttaatgtgaa ccaatgccaa tgggactgct gttgttcttt
    12301 acgggaaaaa tcgacccggt tggataaata atctgctaat ttcggataag aacgcttaaa
    12361 atccataaac tgttcctcct tcgtggaaaa ggtaggttta gtatacgaaa aataaaaaag
    12421 aaatacatcc gtcttaagag ggagtttttc actgaaagtg gagaaaaaag aatggtaaat
    12481 taaaatattc tcagttatac tgaaaattat accaaatttc aaagaagaaa gaggatggga
    12541 atgaaaggca aactaaaacg agaaatcaat ctgtttggcg cattagctac cgttatggga
    12601 accgtgatcg gtgcaggcgt cttcttcaaa actgctgctg ttactgctag tactcagtct
    12661 acaagtttga cattgctagc ttggcttttg ggtggtttct taacgatctg tgctggattg
    12721 acagtggctg aattagctac agcaatcccg gaaactggtg gagctgtaaa atatattgaa
    12781 gctgcgtatg gaaaattgcc cagcttttta ttaggatggg cgcaaagctt gatttacttt
    12841 cctgctaata ttgctgcatt atcgattatc tttgcgacac aattgacgaa cttattacaa
    12901 ttatctaccg attatctttt gttgattgca gtgatcaccg ctgtttctgt gacagggttg
    12961 aatttgttag ggacaaaagt cggtgcttct gtacaatcag tgacgttgat tgttaaatta
    13021 ataccaattg ctgtgattgt catatgggga ttattgaccc caggacaagg aactgtccag
    13081 ttatttccaa tcgaagctgg aaaagatgtt acttttgtcg aaggattgag cagtgcgttg
    13141 ttagcaacgt tgtttgctta tgacggctgg ttaggtgtcg gtgcaatggc aggggaaatg
    13201 aaacggccgg aaaaagattt gcctaaagca attattcttg gtttgagttt tgtaacagtc
    13261 gtttatctat tgatcaattt tgtttttttg aaaactttgc cgattgatca tcttgccggt
    13321 aatttgaatg cagcttctga agcatcagac gtcatttttg gaggaatcgg tggaaagctg
    13381 gtcactattg ggatcttgat ctcggtatac ggtgcattga acggttatac actaactggg
    13441 atccgcgtgc cttatgcgat ggctttagaa gacgagctac ctttcagtaa acagctgaca
    13501 aagctttcaa agaagtttac ggttccttat gttcctgcgg ttttccagtt agcagtagct
    13561 tgtattatga tgagccttgg ttctttcgac tttttaacag acatgttgat ttttgtgatg
    13621 tggctattca gtttactgat ttttatcggt gtctttattt taagaaaaaa agcgccggaa
    13681 ttgccacgtc catataaagt accactttat ccaatcgttc cgattattgc gattctcggt
    13741 gctattttca ttttaggtat gacgatgatg acgcaaacga aattagcatt gatcggtata
    13801 ggtgttacct tgataggtat cccagtctat tatcaaaaaa agaaaaaacg ctcagaagag
    13861 taaaatattt caacaacaga cagaaaaagt acgaactagg tcctctagat cttcgtgctt
    13921 tttctatttc aataaaacaa gttcttctat tttgattttt gctacttctg caagtagtgc
    13981 ttatgtgcta aagtaagaat gaaaaggaga gataaacatg atatttgatt ctcataccca
    14041 tttaaatgct gaacagttca atgaagatat tccagaaacg attcagcgag cacaagaaat
    14101 gggggtaacg aaaatggcgg tcgtgggctt cgatacgcca acgattgaaa agtctttgca
    14161 attaagccat gactatccaa atatatacag tattatcggc tggcatccga cagaggctgg
    14221 cagttatacg aaagaaatcg aaaataaatt acaagaacaa ttgactttac caaaggttgt
    14281 agcattaggg gaaattggct tggattatca ttggatggaa gacccgaaag aagtccaaga
    14341 taaagtattt cgcagacaga ttgctatcgc aaaagaaatg aacctaccaa tcagtattca
    14401 tactagagaa gcacttgaag acacgtatca aatcctaaaa gaagaagatg ttcgagatat
    14461 cggtggaatc atgcatagtt tcagtggtga ttatgaatgg gcaaaacgat ttcttgactt
    14521 agggatgcat atttcattta gcggtgtcgt cactttcaaa aaagcattag acgtccaaga
    14581 ggctgcagca aatgtaccaa tcgaacgatt acttgtggaa acagatgccc cttatctggc
    14641 accagttcct tttagaggaa agcgaaatga accaggctat actcgttata cagtagaaaa
    14701 gattgctgaa cttcggaatc ttccgacaga agaagtggct gcccaaacat ggacaaatgc
    14761 gcaccgatta tttggtctaa gcgaacatgg ataagatgaa gatccaagag attatagtcg
    14821 ttgaaggaaa agacgataca agaaggctgc gggaagtagt tgatgcagat acgatagaaa
    14881 caatagggtc tgcaatcaat gaagagatcc tgatgcaaat tgaacatgcc caagagactc
    14941 gtggagtgat cgtttttacc gatcctgatt tttcaggaga aaaaataaga aaaatcatta
    15001 tggaagctgt tcctcaggca aaacatgctt ttttgccgcg cacacaagct aaaccaaaga
    15061 aaaagggaag ccttggtgtc gagcatgcaa gtgatgctgc tattttagaa gcattgaaaa
    15121 aggtcgtgac gccagacatt tcccaaacgg ctgtttctga aatcactaga caagaccttt
    15181 tagactatgg tttaatagct ggggcacaag caaaagaaaa acgagaaaaa ctaggtgacg
    15241 agttgcgcat cggttacacg aacggcaaac aattggaaaa acgtttgaag atgtttcgta
    15301 ttacgccgaa agaactcgcc caagcaatga aaatagtgga ggaacaacat gagtgaatat
    15361 cgtgaaattg cgacaccctc tagaacaaaa gagattttat tgaaacatgg tttttcattt
    15421 aaaaaaagtt taggacaaaa ttttctgact gaaccaaata ttttaaggaa aatcgtagaa
    15481 actgcgcaaa tagatgaaca tacgaacgtg atcgaagttg gaccaggaat cggtgcattg
    15541 acggaacaat tagccaagca tgcaaaacaa gtcgtagcat ttgagatcga tgatcgtttg
    15601 atccctgtat tggctgatac gatgcaaccc tatgacaatg tgactattgt acatcaagat
    15661 attttaaaga ccgatttgtc aacagctgtt agagaaacat ttcatgagga gcttccgtta
    15721 aaagtcgtag cgaatttacc ttactatatt acgacaccga tcatgatgca ttttcttgaa
    15781 tcagatttgg ttgtggatga actagtcgtc atgatgcaaa aagaagtagc tgatcgtatc
    15841 tctgctgaac caggaacgaa agcttatggt tcattatcga ttgctgttca atattatatg
    15901 gaagccagtt tagcattcat cgtaccaaaa acagtatttg tccctcagcc aaatgtagat
    15961 tcagcgattt tgaaactgac aagaagagat ataccggcag tcgaagtgac agacgaaaaa
    16021 gcatttttcc gattgacaaa agcggcattt cagcaaagaa gaaaaacgct ttggaataac
    16081 ttgcagcatt cgtatggcaa agatgaccag acaaaagcat ggttagcaaa aagcttggaa
    16141 acagctggaa tcgatccaaa acgtcgtggg gagactttgt cattacaaga attcgccgca
    16201 ttaagcaatg cgatgtcaga aaatatgcag taaaaaaaga ggttgtgaca gaagttgtga
    16261 aaaagctgtc ctgcatcaag taataagaac agccaaacaa aaatagttcc tcatgttttt
    16321 cgtttgtttg ggcttgttac cgcagatgca agcttttgaa caccgtttaa gaaagaggtt
    16381 gtgggcctgc cgtttaactc taagtcacaa ggaacaattt caaaaaacag cttctcatgt
    16441 ttttgagtta gttggtctta ttacttgatg caagcttttg accactatta tgccgaacaa
    16501 ggagatgtga cacaaatttt gtcacatctc ctatttttgt ttcttgatca aaaaattctt
    16561 atacttgata ctcacactca ttttgtatgg atttagaaaa gaatatgtgt cttgacagtt
    16621 attagtaagt gagttatact aagtcgacat ttatgtgtca agacatatta ggagagagaa
    16681 aaaatgagaa aaaattcagt tcgaaaacta acgatttctg ctttattgat tgggatgggg
    16741 gtcattatcc caatggtcat gcctaaaatc atgattccac cagcttcctt tactttggcg
    16801 tctcatgtgc cattatttat tgcgatgttt tttacaccag gagttgcagt ggctgttgcg
    16861 ctaggtacga catttggttt ctttttaaca acgccggtta tcatcgcatt gcgagcattg
    16921 tcacatcttg tgtttgcgtt gattggggct tggtacttgc aaaagcatcc gagtatcgtt
    16981 ttaaaaaatg gacagtttac ttttgctaat tggcgtttcc aagggttcaa ttttgtgatc
    17041 ggtgtgattc attctttagc agaaatgcta gtagtcagca tcttttactt tattggtaat
    17101 atgcctgaaa catattattc tcaaggatat ttttatacag tattcatctt gatggggatt
    17161 ggtgggctta tccacagttt agttgattat aatattgctt attttatagc aggaacactc
    17221 agcaaatatt ttgatatccc cgtatttacg gcagcaaaaa aagaaaatcg gaaagagtcg
    17281 attcaagggt acgcgaagaa aacgacatat tagacatgat ctctcaaaaa aaaaactctc
    17341 atctagtaat ggataataaa tcctctaaga tgagagtttt ttgtcctaaa aagagctgca
    17401 ccaacatctt ctggcacagc tctaacaggg ggcaatttat ggaagtttga tgacttgttc
    17461 tgtttcttta tcaaagaagt ggcctttgct gatgttgaag gcaaggtcga ctatttcgcc
    17521 tggtttatgg aaatctcttg catctacttt tgagatgaac tcagtagaac cagtttttgt
    17581 gtaaagcatc gtttcagctc caagtaattc ggaaacgaca acttctgatt tcactacggc
    17641 ttcaggtgaa gcttcaaggg ctacttgttc actgtggatg tcttctggac gaataccgaa
    17701 gataacttct tttccttcat aacctttttc aactaatact ttgttttttc cttcaggaat
    17761 acgaagctta agcccatgtc catctgtgat aacaccattt ttcaatgtca cattaaagaa
    17821 gttcatggca ggagatccaa taaagccggc aacgaacata ttgacgggtg tatcataaac
    17881 ttctttcggt gtaccgattt gttggataaa tccgtctttc atgataacga ttcggtcagc
    17941 catagtcatt gcttctgtct gatcgtgagt aacatagatg gtcgttgttt caagacgctg
    18001 gtgaagtttg gcaatctcag cacgcatggc tacacgtaat ttagcatcta aatttgataa
    18061 tggctcatcc attaagaaga cttttgcatc ccgaacgatt gcccgtccca atgccacacg
    18121 ttggcgttgt ccaccagata aagcagctgg ttttcgtttc aagtattctg tcagacctaa
    18181 gatctctgct gcattgtcca cacgtttttt gatgtctgct ttatcatatt tacgtaattt
    18241 caagccaaaa gccatattat caaaaacagt catatgagga tacaatgcgt agttttggaa
    18301 aaccatggca atatcacggt ctttaggtgc cacgtcattc atgacttttc cgccgatatt
    18361 tagttctcct tcagagatat cttccagtcc cgcgatcatt cgcaaagtag ttgactttcc
    18421 acaaccggaa ggaccaacaa aaacgataaa ttcacggtct ttgattgata gattaaaatc
    18481 agttacagaa taattttctg cattgtcata ttttttatat acgtttttca gagccatttc
    18541 taccataata cgttccacac tttctttttt agttttatta actatttaaa gtataaatga
    18601 aagcgttaac taacttcaat gtcatgttga acaagaattt tttctttttt ttgtcaatgt
    18661 gctagtttta agctttaata aatatccata agaaataaaa actgatacac tggagtaaat
    18721 aagtatgtta acaatatcaa aaggagtgtc tcttatgaaa atcgctttga tcgctcatga
    18781 ccgaaaaaaa cctatgatgg tgaaattagc aacagcgtat aaaacaatat tggaaaaaca
    18841 tgaacttttt gctaccggga cgactgggca gaggatcacg gaagcaaccg gcttgccgat
    18901 ccatcggttt aaatccggac cgttaggcgg ggatcagcaa attggagcca tgatttcgga
    18961 ggatcagtta gatttggtga tttttcttcg agacccgctg gctgcacagc cccatgaacc
    19021 agatgtaaca gcacttatcc gtttatctga tgtctatgag attccgttag ctacgaatat
    19081 cggaacagct gaggtgctac taagaggact ggaagccggc ttcctagatt ttcgaagaat
    19141 cgtccatgaa atagacaagc atccactttc attttgatgg ataagcgtat ggaaaaataa
    19201 cgtttaatgt tcgcctatat gtagctataa aagctaaaca aactgataat atgcgatata
    19261 atacgaacgt attggataat ataaacaata atattagttt ttcgtatttg cagtaaatgt
    19321 gaaaatatgc tatagtttct gtgggaaaag atcccaaaaa taatacagag tagaaaataa
    19381 agcgttaagt gctaagagga tgggatgttg cctcttggac gaaggacgta atgttcgcgg
    19441 ttttattttc gcattcgctg catgatacga tgtagcagct cttaaaggag atgctaaaaa
    19501 tgaagaaaca ccggttagac gcacgtatga ttgctattat gggcttattg attgcattga
    19561 tggtcacttt gtcccgactg gtagcaatcg agaccccatt tattaaaatc agtgtcacat
    19621 ttattccaca agtgatcatg ggcattcttt ttggtccttt ttggtcaggg ataggcgcag
    19681 ttttagctga tttagtagga atggccctct tctcgaaatc tgcattcttt attggattta
    19741 cattgaatgc atttattgaa ggagcaatct acggattctt tttctatcga aaagagatca
    19801 cttggaaaaa tgccattctg gctacgctta gtgtcacgct gatcatcaac ttatttttga
    19861 caccattatg gcttgcgttg atgtaccatg tgccattgtt cagctgggtc gtttgggcgc
    19921 cccgcctgtt aaaaacagta atatggttac cgattcaatc aattgccatt tattacgtag
    19981 gacgtagtat tccttacaaa aaaattttaa gaagtttagc gatccacgct aaataaaaaa
    20041 caagcagtct tcccatccca gaagactgct tgtttttatt gtgctgtgaa ttgtgcttga
    20101 tagagccgtc ggtaataacc atctttttga agcagctcat catgggtgcc catttcgaca
    20161 atagctccct gatccatcac taatatctgg tccgcatttt tgattgtgga caggcgatgg
    20221 gcaatcacaa aactggtttt tccttccatc attgctaaga aggcttgttg gatttttttc
    20281 tctgtcaatg tatcgactga gctggtagct tcatctaaga tcaacatagg gggattactg
    20341 atcatcgttc gagcaatcgt caacagttgg cgttgtccat ctgatatctt taacccttga
    20401 ccgccaatct tagtatctaa tttttccggc aaacgttcta caaattcata catataagag
    20461 gcttttaggg catcattgat ttctgcgtca cttgcttccg gattcccaaa acgaagattt
    20521 tctcgcaatg tactgtcaaa tagccaagta tcttgtaaca ccattccaaa acttttacgt
    20581 aatgtatcac gttggataga gcggatgtca tggtcatcga ctgtaattgt tccttttgtt
    20641 gtgtcataaa aacgcatcag caaattaaca agtgtggatt ttccggcccc tgtttttcct
    20701 actatagcaa tggtttgacc tggataagca gtaaaattga aatcttcgat caaacgctgc
    20761 ttggggtcat aagaaaaaga aacatgatca aaagagactt ttcctttgat atttttgggt
    20821 tccaaggcat aggcatctgg caaatcaggc ttttcttctg gctgatcgat caaatcaaat
    20881 gctcgttcta gaccagaaaa agcggtttgc agttgtgtcg tgattccaga aagttcgata
    20941 aatggtttag aaaactgact tgagtagatc gtaaagctgg aaatcacacc aacagtgatt
    21001 gacgaaccac tcttcaatgc caataggccg ccaactaaac caatcgataa ataagcaagg
    21061 tgatctacaa aacgagaaga cgggtttgtc aaggaagaag aaaattgtgc ccgttgtcct
    21121 ttttcatata gatcagcatt tagcagttca aattgctgtt gactagcttc ttcttgctga
    21181 aaagctttca cgattttctg gttaccgatt cgttcggtca caaagcccga gatatcccca
    21241 ataatccttt gctgtctatc gaaatttgtt tgtgaagcac gagcaacgat ccaactcaca
    21301 aggaaaataa ttggtgtaga acaaagaacg accaatgtca gaagtggact tagacgcatc
    21361 ataaaaataa acgaaagaat gatgattgcc actccagaaa ataattgatt gaaggccgca
    21421 gaaattgcaa tggaaatatt gtccatatca tttgtaaatc gactgacgat atttccgtgg
    21481 gagttttgat cataataacg aagtggcaat cgattcagat gagcaaatgc atcttttctt
    21541 aattttgcaa cggataagta agctaaccga ttgcttaaca cttggatgag ccattgactg
    21601 actacgttga ttaaaacgat accaccgaat agaataagta aatggaacag tcgattgaag
    21661 cggacctgtc cttctccaat catttgatcg atactttgac caatttgtaa cgtcatcaca
    21721 actgttgcaa tcccattgat gattcctagt atcacagcta gccaaatgtc ttttgcatag
    21781 gcagtaaggt aaggaatgaa tcggcggagc gtctgcaagg ataatttttt tactttcata
    21841 actattcttc ctcctgtgat gcgacgattt cctgatattc tgctgattga gcaagtaatt
    21901 cttcatgggt accctgagca gcaatcttac cttgatccat cactaagata tgatgtgctt
    21961 cttgaataga gcggacacgt tgggagataa taatcactgt actgccaaga taagtctgta
    22021 gcgccttacg caaatccaag tctgtttgat aatccaacgc acttaaagag tcatctaaaa
    22081 tcagtaatgc tggcttggca atcaaagctc ttgcaatcgt cagtcgctgt ttttgtccgc
    22141 cagagaaatt tttcccattt tccatgactt ttgtatctaa accatttgga agctgacgga
    22201 caaattcttc tgcctgagcg attttcagag cttcccaaca ttctttttct gtggcgtctt
    22261 tcttacccca ttgaaggttt tcacgaatag tcccggtaaa tagtacagaa gtttggggaa
    22321 caagagcaat ttgctgacgg agatgatcca gctgccaaga acgaacatcc attccgtcca
    22381 ttataactgt accggaactg acatcataga aacgcggaat caattgtgtc aaagtcgttt
    22441 tcccgcttcc ggttggacca atgattccaa ggactgtatt tcgcttcagc tgaaatgtaa
    22501 tattttggag agaaagacca gaatcaggag tataacggaa gtcaacatgg tcaaaagcga
    22561 taattgcttc agaagaaaaa tcgggagtag tactgacctc agcttcaaga atcgaatttt
    22621 ctgtgtctag cacctctttc acccgatttc ctgaagcttc ggcacgtgtg aagatcacga
    22681 cgaggtttga aacaacgatc agtgccaaaa gcatttgatt catataattg atcaaagcaa
    22741 gaacttgtcc ttgttgcaag ctaccaatat ttactttgat cccgccaact gtcagcaaac
    22801 agatgatccc gacattcatg atcaaggttg tagcaggtga taatagggca gagatattgg
    22861 aaacacgtaa ataattgttc gctagatcat ttgtgctttt atcgaacttc tctgtttctt
    22921 tttttgtctg cgcaaatgcg cggatgacac gaacaccgct aagattctga ctaacgtgtt
    22981 cattgagctg atctagtttt tcttgcactt tttggtagag aggaacggtt tttttgatga
    23041 taaaaaataa aatgattgag aaaataggca ataatgctaa gaaaaacaga cctacttccc
    23101 agtcgatcac aaaagccatg accactgacc cgatacttaa aaacggtgct ctgatcaata
    23161 aacgaatgac cattgctaaa gctagctgaa gctgattgat gtcgtttgtc attcgggtga
    23221 tcaaggtatc tgttccaaaa tgattcaact cgttgtgaga gaaagtattg atttttttga
    23281 ttagctgatt acgtaactct gtaccaaatc cttgtgaagc tactgaagca tagtattggc
    23341 aaatgatagc acaaatcaga ccaatcacag acatagcgac catccataag cccatttcaa
    23401 tgatttttcc tcggtcattc atttttagtc cattatcgat caaggaagcc atcattaaag
    23461 gcaggacaag ttcgaagaca gcttcaagaa atttgaagaa cgggcccaaa atgatttgct
    23521 tacgatagtc ttttgcataa cgtaataact gaatcatatg attcctccta ttatttattt
    23581 gtccacaatt gaaaaaacaa gtactaccac attgtagctt aggaaattta gaatgaaaag
    23641 agaacaagcc ttttcataaa tgaaagtttt attatgaaga agctttagaa attgaataga
    23701 ctattttttt taaaaaaaag aaaatgagaa aacatgccag tctcttcatt cataggaacg
    23761 tctattttac ttaattttt
//
LOCUS       DARBET010000037        23064 bp    DNA     linear   BCT 07-NOV-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4027
            SAMEA114569841-rid20112183.denovo.037, whole genome shotgun
            sequence.
ACCESSION   DARBET010000037 DARBET010000000
VERSION     DARBET010000037.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA114569841
            Sequence Read Archive: ERR12203666
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 23064)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 23064)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001972618.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001972618.1
            Genome Coverage       :: 132x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/07/2023 13:18:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,041
            CDSs (total)                      :: 2,975
            Genes (coding)                    :: 2,908
            CDSs (with protein)               :: 2,908
            Genes (RNA)                       :: 66
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 67
            CDSs (without protein)            :: 67
            Pseudo Genes (ambiguous residues) :: 0 of 67
            Pseudo Genes (frameshifted)       :: 26 of 67
            Pseudo Genes (incomplete)         :: 28 of 67
            Pseudo Genes (internal stop)      :: 20 of 67
            Pseudo Genes (multiple problems)  :: 6 of 67
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..23064
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA114569841-rid20112183.denovo.037"
                     /isolate="VRE4027"
                     /db_xref="taxon:1352"
                     /country="Denmark"
     gene            complement(110..565)
                     /locus_tag="SCJ86_001629"
     CDS             complement(110..565)
                     /locus_tag="SCJ86_001629"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290187.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEG1169170.1"
                     /translation="MSKEMTALKFYFRNGETWTINRRHIGDLWIKQITTSFGRINGSE
                     FVEIHPCAGFKIEIFHEGDAVATHDINLGGLEMGMFNRALKYEDIERMEILYRNGTPD
                     LVYFPYLDKGTEGLDNQYQSTKISEKTGNLYIVINPDQRVEDVYGEFFE"
     gene            complement(719..1927)
                     /locus_tag="SCJ86_001630"
     CDS             complement(719..1927)
                     /locus_tag="SCJ86_001630"
                     /EC_number="2.7.7.72"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344136.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CCA tRNA nucleotidyltransferase"
                     /protein_id="HEG1169171.1"
                     /translation="MKLKQLPLEYQKAIPVLKKLENAGYEAYFVGGSVRDILLNQPIH
                     DVDIATSAFPEEIKQLFPKTIDIGIEHGTVLVLENDEQYEITTFRTESTYQDYRRPDH
                     VEFVRSLAEDLKRRDFTINAFALKEDGEIIDLFDGLQDLEEQILRAVGNPHERFHEDA
                     LRMMRGLRFVSQLGFKLEEETFYAIKENHELLGKISVERINVEFIKMLLGNEGAAGIH
                     AFIETECYICCPGLKEEGEALLRFAELPSVPLKEESQAWALLISQLDLKENEIRSFLK
                     SWKCSNQMIKDVQALVQGLRQRETGALEPFELYFLGKKHALQVEELMIYFDETPQMDA
                     VGESYDRLPIKSLSELAVDGKILLEETGKKPGKWVSEALQLAEKAVVEQRIKNDKKQV
                     LDFLSKEKLI"
     gene            complement(2039..2371)
                     /locus_tag="SCJ86_001631"
     CDS             complement(2039..2371)
                     /locus_tag="SCJ86_001631"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290045.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotide pyrophosphohydrolase"
                     /protein_id="HEG1169172.1"
                     /translation="MDKKRSLYSMQQEVDEYIQQFKVGYFSPLAQMARLTEEVGELAR
                     EVNHTYGEKSKKASEPVNSVAEELGDVLFVTMIMANSLNIDLTEVFEKNMEKFNQRDH
                     NRFERKDE"
     gene            2519..3394
                     /locus_tag="SCJ86_001632"
     CDS             2519..3394
                     /locus_tag="SCJ86_001632"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YitT family protein"
                     /protein_id="HEG1169173.1"
                     /translation="MVKRKTVKEVLFIIIGTSIYAFGLVYLNIANHLAEGGVSGITLI
                     LRALFGIDPAYTTLLINIPLILLGGKILGKRSLAYTVLGTLSLSFFLWIWQRVPLEIN
                     LQHDLLIVSLLAGLVAGVGSGIVYRMGGTTGGSDIIARILEQNYGITMGRSLLAFDII
                     VLLASLTYIDLKRMMYTLIVSYVFSRVIDSILDGGYSAKGLLVVSNKSEEIAPLLMTG
                     LQRGVTFLSGEGAFSGNSKKIIYMVVSSSELTEAKRIVHEIDEKAFLSIINVHEVEGQ
                     GFTYLKPQTRLLKKL"
     gene            complement(3476..4057)
                     /locus_tag="SCJ86_001633"
     CDS             complement(3476..4057)
                     /locus_tag="SCJ86_001633"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289878.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YpiB family protein"
                     /protein_id="HEG1169174.1"
                     /translation="MLIDVREKKNFLTWMVNHVSFSRREVFWILNYLANHEAILSNVH
                     FVEGANFADRGLQISDLSVEGEPMKLFLQGKEFTDTDQIFHEIRLNWKKPLYVECIFE
                     NAWQTREYLSILEDNPFSSWDSSVSEEDTELIERYFQEKEEEAKLKMLYAQIDQALED
                     GDRDAFLKLSDEVNRKILLQTQKKSNRVSEHNN"
     gene            complement(4063..5322)
                     /locus_tag="SCJ86_001634"
     CDS             complement(4063..5322)
                     /locus_tag="SCJ86_001634"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289879.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HEG1169175.1"
                     /translation="MNTNSEKMLQALSDEDLSQAQIFLEKALKEDPADILAELGEELL
                     AIGFLIEAKQIFEQLINQYPENDGLNIPLAEIAIEDNEIDLAFEYLEKVDKQSDYYPQ
                     SLLAIADLYQVIGIPEVSEAKLKEAAGLLPDEPLIQFALAELYFSVDRFKEAAVVYET
                     LLASGINEISGISMQERLGQSLSMQGEFEAAIVPLEEALKEERTDDRLFQLAFTNLQL
                     KENQQAINYLEELREVNPHYQSLYLYLGQALQEEEMIEEAQTVLEEGIKENPYQVELY
                     HLASENAFRLHDRQRAEELLLKALEVGDKQDETLLTLSNLYLDDERYEDVIQVINQME
                     ETANPYAEWNLAHAYNELEDFTVAAVHYEQAYHELSHEPDFLKEYAFFLREEGQLKRA
                     QELLTYYLTLEPGDMEALSLLDDLTER"
     gene            complement(5324..6370)
                     /locus_tag="SCJ86_001635"
     CDS             complement(5324..6370)
                     /locus_tag="SCJ86_001635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEG1169176.1"
                     /translation="MELDYQVEDALTKVFYGETLVSQLRELSIDQPILFLTNQRYYDL
                     FADKINPLFANQANNDWYICANTQCNHLTELKNLLDFVKRYPENQSMFIIGFGNEGIM
                     DLAGFFQKHTTLDSSLWMIPVSIRSLSRALTPQRTILQLPNRVVLQTINLPERIVYDQ
                     TISERQVDGKQIDFLTFICCGMICDHRFLQNLYKNYPNKKQLTTRPFAGMIEEMIYFY
                     QLEAENLSNYGKLFEQAFYLTENGHLLSASMKKFLGMMFHFVWNLQAYPFDFQLKNFF
                     VWLDLLGYPIHWLEQISKMEYLEKVLFLAEKSKKILVLEKIGIIDGYQHPNEKELLRT
                     MTVFQQIVSEIRGN"
     gene            complement(6605..6880)
                     /locus_tag="SCJ86_001636"
     CDS             complement(6605..6880)
                     /locus_tag="SCJ86_001636"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007208657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HU family DNA-binding protein"
                     /protein_id="HEG1169177.1"
                     /translation="MANKAELIEKVASATDLTKKDATAAVDAVFSTIQDALANGEKVQ
                     LIGFGNFEVRERAARKGRNPQTGEEIEIPASKVPAFKPGKALKDAVK"
     gene            complement(7123..8433)
                     /gene="der"
                     /locus_tag="SCJ86_001637"
     CDS             complement(7123..8433)
                     /gene="der"
                     /locus_tag="SCJ86_001637"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005878566.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome biogenesis GTPase Der"
                     /protein_id="HEG1169178.1"
                     /translation="MANPTIAIVGRPNVGKSTIFNRIAGERISIVEDTPGVTRDRIYA
                     KGEWLGREFSVIDTGGIDLGDEPFMDQIKHQAEIAIEEADVIICVVSGREGVTDADEM
                     VAKILYRSNKPVILAVNKVDNPEMRNDIYEFYSLGLGDPYPVSGSHGLGIGDILDEAV
                     KYFSEETEEEDDDTIKFSLIGRPNVGKSSLINAILGEERVIVSDIEGTTRDAIDTHFV
                     SESGQKFLMIDTAGMRKRGKVYENTEKYSVMRAMRAIDRSDIVLMVLNAEEGIREQDK
                     RVAGYAHEAGRGIIIVVNKWDLVKKETNTMRDFEQEIRDEFRYLDYAPIVFVSAVTKQ
                     RLERLPEMIEQVSMNQNLRISSAVLNDIIMDAVAINPTPTDKGKRLKIFYATQVAVKP
                     PTFVVFVNEEELMHFSYERFLENQIRKAFTFEGTSIRIIPRRRK"
     gene            complement(8615..9844)
                     /gene="rpsA"
                     /locus_tag="SCJ86_001638"
     CDS             complement(8615..9844)
                     /gene="rpsA"
                     /locus_tag="SCJ86_001638"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008265592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S1"
                     /protein_id="HEG1169179.1"
                     /translation="MTEFDQNQENNQSMEDAMKSVQEVKVGDVVKGEVLVIEDKQVIV
                     GIEGAGVEGVVPAKELSTLPVEDISEVVKVGDVLDLVVISTIGKDKENGSYLLSKRRL
                     DAKKVWEDIERDFQEGKVIEAPVTNVVKGGLVVDVGVRGFVPASMVEDHFVADFSDYK
                     GKTLSFKIIEIEPSENRLILSHKAVVENEREEKKKEILSTIHEGDVLSGRVARLTDFG
                     AFVDLGGIDGLVHVSEISHSHVAKPSDVLAVNDDVNVKVLSINPEQERVSLSIKDTQP
                     GPWSEIDTKAPVGTVLDGTVKRLTSFGAFVEVFPGVEGLVHISQISHKHIATPHEVLH
                     EGDEVKVKVLEIHPEEHRIALSIKALEEKPASEEEPKNVESYELPEENTGFTMGDILG
                     DALKEEVEEKATDDSEK"
     gene            complement(9979..10659)
                     /locus_tag="SCJ86_001639"
     CDS             complement(9979..10659)
                     /locus_tag="SCJ86_001639"
                     /EC_number="2.7.4.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="(d)CMP kinase"
                     /protein_id="HEG1169180.1"
                     /translation="MTQISIAIDGPASSGKSTVAKILAKELNYIYTDTGAMYRAVTYL
                     AITNNVSFSDEEGLVSLIKKYPISFSQQEDGQHVFIASKDVTLEIRQPDVTKAVSEVS
                     AHGKVREELVAVQRKIGERGGVVMDGRDIGTAVLPNAEVKIFLVASVSERAERRFKEN
                     QEKGIPTDLETLTKEIQERDHYDSTRDVSPLKQAEDAVRIDTTGKSIPEVVAAIKAVV
                     LKKGYLLF"
     gene            complement(10708..11343)
                     /locus_tag="SCJ86_001640"
     CDS             complement(10708..11343)
                     /locus_tag="SCJ86_001640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315217.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysM peptidoglycan-binding domain-containing
                     protein"
                     /protein_id="HEG1169181.1"
                     /translation="MSRKDRHQSTENNETQEPWEQPIYDTDDEVSSRSSQRQQKKGNT
                     LFLSLFLILLVLCIAIPAGAYFWIRNGSSNNATAATSSETSTSVVESSTSTTKEESTE
                     TSTVESSTIAEESTVSQDTGVTSESSIAAETTPTSSSEATYQAGDSSSNAAETTPSSS
                     AAAGSTTTVQAGEGPKQVAERAGITTDQLFELNGLDPNNFMLYPGQELRIK"
     gene            complement(11409..12812)
                     /locus_tag="SCJ86_001641"
     CDS             complement(11409..12812)
                     /locus_tag="SCJ86_001641"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent DNA helicase RecQ"
                     /protein_id="HEG1169182.1"
                     /translation="MLINELKKYFGFDSFRPGQEEIIQALLDGEDTLAILPTGTGKSL
                     CYQLTGYLMEGLVIIVSPLLSLMEDQVTQLQKRGEKRVAAFNSLLSQSERRYVLRHLS
                     QYKFLFLSPEMLTNPSLLEHLKKQNIALYVVDEAHCVSQWGVDFRPEYQQLGKIRKLL
                     GNPVTLALTATATDLVARDIRHVLFDRPPKEIRQSVNRQNISLFVRKTQQKEQELEHF
                     MERAQGAAIIYCATKKEVERLYHLFRERFTVGYYHGGLDAAQRRQLQQQFVKNQLQFL
                     IATNAFGMGIDKSDIRYVVHYDLPDSLENYVQEIGRAGRDQEESAAILFYQSGDERIH
                     YFFNQLSREQRQSFELYLEYATKQAPFDELQKKWMELIQQSEKPENWVERLKRQEKEK
                     EFRLQQMLRYINEENCRRKFILAYFGEKLSEKPQNCCDIDGAKAELLPKRKLFSKTEQ
                     LHWETILLNLFKKNMEH"
     gene            complement(12799..13830)
                     /locus_tag="SCJ86_001642"
     CDS             complement(12799..13830)
                     /locus_tag="SCJ86_001642"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HEG1169183.1"
                     /translation="MIVFILSLFSSGHKLRISSLYQLLVGKRTTSVLIYGFTHELLFI
                     HNSFPELKQDKFYQILQKIAQQGWIEINENEAKLTPAGADMLSEHQIEHTGLRFDRYG
                     RTGETSWRLIKFAVQVISNLATGIQDYLPAETSPFYTFQLKKWLSESQLPREILIDTA
                     YESLVQLFSEIPEEAADFLANQLSGNERTGLLPYQLAKNNDESEVYLQQSRCIHLLLA
                     QIEKRPDSLWHALIDSLLQQNFNQSMMITKQMFMNGQTIDQIMAIRHLKRGTVTDHLI
                     EWALFFDDFPYEWILSAETIERLEPNKDSVREWRFSEWNVDGQLDYGEFRLYQIYLLR
                     KEAIQNVNK"
     gene            13861..14082
                     /locus_tag="SCJ86_001643"
     CDS             13861..14082
                     /locus_tag="SCJ86_001643"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294401.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferredoxin"
                     /protein_id="HEG1169184.1"
                     /translation="MSLLCKLVPERCIACGLCQIEAPDIFDYDEEGIVLFAQEPEAHQ
                     QFVMPEEETYVKKAYQKCPVRAILLENKI"
     gene            complement(14247..14984)
                     /locus_tag="SCJ86_001644"
     CDS             complement(14247..14984)
                     /locus_tag="SCJ86_001644"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301243.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioesterase"
                     /protein_id="HEG1169185.1"
                     /translation="MKIFRRILIGLVIILGLLGGAGYYYIQQNTYEPSSEAQVLVKEG
                     KNKEDYLFFEGEKKNHGKSPLLIFYPGALVEPESYSIWAQDLAKNGFSVAIVKMPLDL
                     AVMGGNKADKVKEDFPNSDYVIGGHSLGGVMASRYVNKNHSDKHLKGVFFMASYPDKK
                     GTLTESAVPVLSITATKDKVLNQEAYQEAKQYLPERTEYVSINGGNHGGFGSYGEQKG
                     DGEAEISNADQQTVIVNTLENWLKKLK"
     gene            complement(15137..16477)
                     /locus_tag="SCJ86_001645"
     CDS             complement(15137..16477)
                     /locus_tag="SCJ86_001645"
                     /EC_number="3.4.13.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306940.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Sapep family Mn(2+)-dependent dipeptidase"
                     /protein_id="HEG1169186.1"
                     /translation="MKKFVTEQHHEQALESLSELIRIPSVLDEADSGQGHPFGKKVIE
                     ALDKVLEISEKLGFRTFKDPEGYYGYSEIGSGDELFGILCHMDVVPAGDENNWETKPF
                     DPTIKDGWLVGRGSQDDKGPSIAAMYAVKALIDAGVEFKTRIRFIFGTDEENLWRCLE
                     KYNEKEEGITQGFAPDAEFPLIYAEKGLLQAYLTGPGTNEFSVKAGGALNVVPDAAPY
                     SGEKLSEVKEALKKHEFDFEEQGEGIVVLGKSIHAKDAAQGVNAISRLAIALSEVFDF
                     GPINFLGKLVQENATGEAVVGKTEDEQSGELTMNFASLEITPEQTKIGVDMRIPVTFK
                     KDDLVAKLTKTAEKYGLTYEEFDFLDSLYVPLDSELVKNLLGTYRDITGDMTEPFVSG
                     GATFARTMNQCVAFGAMFPDTPDFMHQANERWELSSMYKAMEIYAEAVYRLCAK"
     gene            complement(16714..18237)
                     /locus_tag="SCJ86_001646"
     CDS             complement(16714..18237)
                     /locus_tag="SCJ86_001646"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016627896.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfcC family protein"
                     /protein_id="HEG1169187.1"
                     /translation="MEQTKKKKFQMPSAYTVLFIIIAVIAVLTWFIPAGHYSVDDAGN
                     IVAGSYERVQQNPQGLWDIFMAPINGMLGVPAGELTKETPAAIPISFFILVVGGFLGI
                     INKTGALDAGIASVVKKFKGKEKMLIPVLMLLFALGGSTYGMAEETIPFYALLIPVMM
                     AVGFDTVVAVAIVLVGSQVGCLASTVNPFATGVASQAVGISMGEGIGLRFLMFVILLA
                     ISIVYVYRYASKIEKDPTKSLVYNQRKEDMKHFDIEAIGRQEVITKKQKHVNVLFFIT
                     FGIMIISLIPWTTLNANFTIFESLTNWLTNLPVLGTVLGKSMLPLGDWYFPEITMLFL
                     VMSIVVAITYGMKESEFVSEFLAGASDLIGVAIVVAVARGIQVVMNNGLITDTILHWG
                     EQGLSSLSSSVFIIITFIFYIPMSFLIPSTSGLAAATMGIMGPMGEFAGVGKDLVITA
                     YQSASGLVNLITPTSAIVMGAVAIARFDVSVWWKFTGKLIAILFVAICVILGVAAMF"
     gene            complement(18615..19226)
                     /locus_tag="SCJ86_001647"
     CDS             complement(18615..19226)
                     /locus_tag="SCJ86_001647"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294393.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ECF transporter S component"
                     /protein_id="HEG1169188.1"
                     /translation="MKNTRVQKMVAVAMFAAIALVLQYFAFPIMPAFSFLKIDFSDIS
                     VLISMFLFGPAAGVATAFLRSVLHLLTTGFSPDNLVGDTASFLSTTIFTFPIYYFFSV
                     KKQHKGRNKFLGVITGTIALTIFMSIANYFVITPLYLMFLGLNATQMLGMSLANYVLI
                     GIVPFNLIKGFIVSAVFLVLHAQLLPWLSRKQHQFEQRHTAVK"
     regulatory      complement(19349..19474)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00050"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="FMN riboswitch; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="flavin mononucleotide"
                     /db_xref="RFAM:RF00050"
     gene            complement(19556..20272)
                     /locus_tag="SCJ86_001648"
     CDS             complement(19556..20272)
                     /locus_tag="SCJ86_001648"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005878594.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rRNA pseudouridine synthase"
                     /protein_id="HEG1169189.1"
                     /translation="MERLQKVIAHAGITSRRKAEEFILAGRVKVNGQVVRELGIKVGK
                     HDTVEVDEVPIYQEEYGYYLLYKPKGVISAVADDKGRKVVTDLLPMVKERIYPVGRLD
                     YDTSGILLLTNDGDFAQRLTHPKHEVDKVYVAKVKGIATKSMLAPLTKGIKIEGRKTS
                     PAKFQILSVDPKTNHSIVELTIHEGRNHQVKNMLQAVGLPVQKLKREKYGDLTLQGLR
                     PGDYRELNKKEVSSLLNHSK"
     gene            complement(20278..20847)
                     /gene="scpB"
                     /locus_tag="SCJ86_001649"
     CDS             complement(20278..20847)
                     /gene="scpB"
                     /locus_tag="SCJ86_001649"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337146.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SMC-Scp complex subunit ScpB"
                     /protein_id="HEG1169190.1"
                     /translation="MTKLSELEALLFIVGEEGISNEELSGLLNLSEAEVTSLVKELKE
                     SYTQNSHSALHIFETESHYVLTTKKELAPLLKRYAQGTSNTLSQAAVETLAIIAYKQP
                     ITRAEIEQIRGVQVSGAVQRLAAHKLIEEKGRVEGPGRPILYGTTGYFLDYFGLKSLE
                     ELPDTQEMEISLEEDVSPDLFFDGMNKGE"
     gene            complement(20831..21628)
                     /locus_tag="SCJ86_001650"
     CDS             complement(20831..21628)
                     /locus_tag="SCJ86_001650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315222.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="segregation/condensation protein A"
                     /protein_id="HEG1169191.1"
                     /translation="MASKEKEERVNELTEINIKLDVFEGPLDLLLHLIQQMEIDIYDI
                     PIATITEQYMNYIHTMKTLELAVAGEYLVMAATLMAIKSKTLLPVQEFGTDDEELWEE
                     DPREELVTQLLEYRKYKYAAERLTEKAEERSLYYTKEPMNIDGLADAEKPLKRGQVKK
                     IDLIRAMQKILERKKFSQKVEKTITPDDTTIEERMVFIEERIKRNAHGCTFDSFFESP
                     SKSEVVTTFMALLELMKKGQIIAQQKENYESIMLYPAVGDTEHDEIK"
     gene            complement(21598..21969)
                     /locus_tag="SCJ86_001651"
     CDS             complement(21598..21969)
                     /locus_tag="SCJ86_001651"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343538.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetyltransferase"
                     /protein_id="HEG1169192.1"
                     /translation="MLTHYRKENRKIAMGLLSFHKKLTGKTSLLKEIDTYESEDDYEL
                     FFFTPEGSTNIQGIIGIYWATNDQLIIHDISLNPSYRGENMGFQMLDELQEKYPDVSI
                     RATEITEPYIGKWLAKKKKKE"
     gene            complement(21988..22875)
                     /gene="xerD"
                     /locus_tag="SCJ86_001652"
     CDS             complement(21988..22875)
                     /gene="xerD"
                     /locus_tag="SCJ86_001652"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309610.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific tyrosine recombinase XerD"
                     /protein_id="HEG1169193.1"
                     /translation="MKEQINDYLHYLNIERGLSANTRQSYERDLEQYLHFLQEQKIAS
                     WQDVDRFLVIRFLEKLHEEKKATATVTRMISSLRRFHQFLRQERITDHDPMQHIDTPK
                     KAQKLPSTLTLTEVEKLIEAPDTTKTLGIRDRAILEVMYATGMRVSELIGLKLGDLHL
                     SLGLVQTVGKGDKERIIPLGDYAIQWLERYLDEARPLLVKDASEMHVFVNNHGKGLSR
                     QGIWKNLKQLVREAGIYKEVTPHTLRHSFATHLLENGADLRTVQELLGHADISTTQIY
                     THITKKRMTDVYKQHFPRA"
BASE COUNT         6467 a         4926 c         3910 g         7761 t
ORIGIN      
        1 aaaatcttgt cacagtccct tattctgaat aaacggtgtg gaagagacag cctatcagta
       61 gtaataagac acatctcttt cacagcctca tttttgcgta aaaatcgatt tattcgaaaa
      121 attccccgta tacatcttct acacgttgat ctggattgat cacgatatac aagttacctg
      181 ttttttcgct gatttttgtt gattgatatt gattgtctaa tccttctgtt cctttatcta
      241 gatatgggaa gtacacaaga tcaggtgtgc catttcggta aaggatttcc attcgctcga
      301 tatcttcgta tttcaatgcg cgattaaaca tgcccatttc cagtccgcct aggttgatat
      361 catgtgtcgc aactgcgtcc ccttcatgga agatttcgat tttaaatcca gcacacggat
      421 ggatttcaac aaattcactg ccattgattc gtccaaaact tgttgtgatt tgtttgatcc
      481 aaagatcgcc gatatgacgg cggttgatcg tccaggtttc tccatttcta aaataaaatt
      541 ttagcgctgt catttcttta ctcattaaaa agactccttt atgatgtaat ttattcttgt
      601 actacctcag tttagcacaa atttatgtaa gtggtcacat cttatgtttt ttcttctaat
      661 gacgtatctg cccactttaa gtagccaaca aagctgagca gatacagtct agtgtttttt
      721 aaatcaattt ttctttcgat aaaaaatcca aaacctgttt tttatcattt tttattcttt
      781 gttctacgac ggctttttca gctaattgca aagcttcact aacccactta ccaggttttt
      841 ttccagtttc ttctagcaag attttaccat ctactgctaa ttcgcttaat gacttgatag
      901 gtaaacgatc ataggattct cctacggcat ccatctgagg tgtttcgtca aaatagatca
      961 tcaattcttc tacttgcagt gcatgttttt tgcctaagaa gtacaattcg aatggttcca
     1021 aagctccagt ttctcgttgt cgtagtcctt ggacaagtgc ttgaacgtct ttaatcattt
     1081 ggttagaaca cttccacgat ttcaaaaagg aacgaatctc attttctttt agatccagtt
     1141 ggctgatcaa taatgcccat gcttggctct cttctttcaa tgggacggat ggtaattcag
     1201 caaaacgtaa aagtgcttcg ccttcttctt tcaaaccggg acagcaaatg tagcactctg
     1261 tttcaataaa tgcatggatt cccgcggccc cttcatttcc caagagcatc ttgatgaatt
     1321 ccacatttat acgttctaca gatattttac ctaatagctc gtggttttct ttgatggcgt
     1381 agaaggtttc ttcctctaat ttgaacccta gttgactgac aaaacgcaac ccgcgcatca
     1441 ttcgtaaggc atcttcatgg aaccgctcat gaggattgcc tacagctcgc aagatctgtt
     1501 cttctagatc ttgcagacca tcgaacaaat cgatgatttc tccatcttct ttcaaagcaa
     1561 acgcattgat cgtaaaatct cttcttttca aatcttctgc caacgaacga acgaattcga
     1621 catgatctgg tctacggtag tcctgataag ttgattccgt ccgaaatgtg gtgatctcat
     1681 attgttcatc attttctaat accaacacag ttccatgttc gatcccaata tctatcgttt
     1741 ttggaaaaag ctgtttgatt tcttctggaa atgcgcttgt tgcgatatcc acatcatgga
     1801 ttggctggtt cagcaagata tcacgaacac ttccacctac aaaataagct tcatatcctg
     1861 cattctctaa ttttttcaga actggaattg ccttttggta ttccagaggt aattgtttta
     1921 atttcatttt tccaaccttt cctgcgtgtt tcttgtcctg agataaagac tcagttgcat
     1981 ggtgactgtt tgacaagtcc gtttcagagt cttcaaggcc aggaccactt catcttgttt
     2041 actcgtcctt acgctcaaag cgattatggt ctctttgatt gaatttttcc atgttttttt
     2101 caaaaacttc agttaagtcg atattcaatg aattagccat gatcatcgta acgaataaaa
     2161 catcaccaag ttcttctgct accgagttaa ctggttcaga agcctttttg cttttttctc
     2221 cataagtgtg gttgacttct cttgccaatt cacctacttc ttcagttaat cgagccatct
     2281 gagctaatgg agagaaatac cccaccttga attgctggat atattcatcc acttcctgct
     2341 gcatactgta taaagaacgc tttttatcca tctttttccc tccaaaacta tcttaccaaa
     2401 actttaacgc aaatcctaga taagagcaat tgagaaaatc gaatttccat gctacattta
     2461 ggaaggaatt tctaaatgga aattctgtga aaatacataa ttgaaagctg gaattcatat
     2521 ggtcaaacga aaaacagtaa aagaagttct ttttatcatt ataggaacaa gtatttatgc
     2581 atttggtctt gtttacttga acatcgctaa tcatttagca gaagggggag tctcagggat
     2641 caccttgatc ttaagagctt tatttggtat tgatccagct tatacgactt tattaatcaa
     2701 catcccattg attttactag gaggaaagat cctcggtaaa cgttccttgg cctatactgt
     2761 tctaggtaca ctttctttgt cattcttttt atggatctgg caaagggttc cactagaaat
     2821 caatttacag catgatctac tgatcgtttc tctacttgcg ggtttagttg caggtgtagg
     2881 aagcgggatc gtctaccgca tgggtggaac aacaggcggc agtgatatta tcgctcggat
     2941 tttagaacaa aattacggca tcactatggg gcgttcccta ttggcatttg atatcatcgt
     3001 cttacttgct tcattaacct atatcgactt gaaacgtatg atgtatacgt tgattgtttc
     3061 gtatgttttc agtcgtgtga ttgattcgat cttagatggc ggatattctg caaaaggtct
     3121 acttgtcgtc tccaacaaaa gcgaagagat tgcaccacta ctgatgaccg gtctgcaaag
     3181 aggtgtgact ttcctttctg gagaaggcgc attctctggt aattcaaaaa aaatcatcta
     3241 tatggtcgtt agctctagtg aattaactga agctaagagg atcgtccacg agattgacga
     3301 aaaagctttc ctttcaatca tcaatgtgca tgaagtggaa gggcaaggct ttacttattt
     3361 gaagccacag acaagattac tgaaaaaact ataagaaaaa agacaaagat aggctatgag
     3421 gaaagattcc acatccagct gacctttgtc ttttttagta ctatactatt tttaactaat
     3481 tgttatgttc acttacccgg ttacttttct tttgtgtctg caaaaggatc tttcggttaa
     3541 cttcatcaga taatttcaaa aaagcatccc tatctccatc ttctagtgct tgatcgattt
     3601 gagcataaag catttttagt tttgcctctt cttctttttc ttgaaaatat cgttcaatta
     3661 attcagtatc ttcttcactg actgaagaat cccaagaaga aaatggattg tcttctaaga
     3721 tagatagata ttctcttgtc tgccaagcat tttcgaatat acattccaca tataaaggct
     3781 ttttccaatt caaacggatt tcatgaaaaa tctggtctgt gtccgtaaat tcttttccct
     3841 gcaaaaacaa tttcatgggt tccccttcaa cagatagatc actgatctgc agtcctcgat
     3901 ccgcaaaatt cgccccttca acaaagtgga cattgcttaa aatcgcttca tgattggcaa
     3961 gataattcaa gatccaaaaa acttctcttc ggctaaaaga tacatgattg accatccacg
     4021 ttaaaaaatt cttcttttcc cgtacatcaa ttagcatttt tctcacctct ctgttaggtc
     4081 atctaataat gacagtgcct ccatatctcc tggttcaagt gttaaataat aagtcaataa
     4141 ttcttgcgct cttttcagct gcccttcttc tcttaagaaa aaggcatact ctttcaagaa
     4201 atctggttca tgtgatagtt catgataagc ctgttcatag tgtacagctg caacggtaaa
     4261 gtcttctaat tcattataag catgggctag gttccattct gcataaggat tagcagtttc
     4321 ttccatctga ttgatcactt gtatcacatc ttcatatctt tcatcatcca agtaaagatt
     4381 acttaaagta agtaatgttt catcttgttt gtctcctact tctaaagctt tcagtaaaag
     4441 ttcttctgct cgctgtctat catgcagacg gaaagcattt tccgaggcca ggtgataaag
     4501 ttcgacttgg taaggatttt ctttgattcc ttcttctaac actgtttgtg cttcttcaat
     4561 catttcttct tcttgaagag cttgtcctag atatagatat aacgattgat aatgcggatt
     4621 aacttctcga agttcttcca gatagttgat tgcctgctga ttttctttca attgaagatt
     4681 cgtaaaagct agctggaaca gtcgatcatc tgtcctttct tctttcagtg cttcttctaa
     4741 aggtacgata gctgcttcga attctccttg catacttaag gattgtccta aacgttcctg
     4801 catcgagatt cctgaaattt catttatccc gcttgccaac aatgtttcat aaaccactgc
     4861 tgcctctttg aaacgatcaa ctgaaaaata caattcagct agcgcaaact ggatcaatgg
     4921 ttcatcagga agcaacccag ctgcttcttt caattttgct tcactcactt cagggattcc
     4981 gataacttgg tacaaatcag ctatcgccaa taagctttgc ggatagtaat cactttgctt
     5041 atccactttt tccaggtatt caaaagctaa gtcgatctca ttatcttcga ttgcaatttc
     5101 tgctaacggg atgttcagtc cgtcattttc tgggtattgg ttgatcagtt gttcaaaaat
     5161 ctgttttgct tcgatcaaaa agccaatagc cagcaattct tcgcctagtt cagctaatat
     5221 atctgctggg tcttctttta atgctttttc taaaaagatt tgtgcttgtg acaagtcttc
     5281 atctgataaa gcttgcaaca ttttttcgct atttgtattc atttcaattc cctcttatct
     5341 cactgacgat ttgttgaaat accgtcattg ttctcaataa ttctttttcg tttggatgtt
     5401 ggtatccgtc tattatacct attttttcta aaacaagtat ttttttgctt ttttctgcca
     5461 aaaaaagaac tttttccaaa tactccatct ttgaaatctg ttccagccag tggattggat
     5521 agccaagcaa gtccagccac acaaaaaagt tttttagttg gaaatcaaaa ggatatgctt
     5581 ggagattcca cacgaaatga aacatcattc ctaaaaattt tttcatacta gcagaaagta
     5641 gatggccatt ttcggtcaga taaaaagctt gttcaaacag tttaccataa tttgacaggt
     5701 tttctgcctc taattgataa aaatagatca tttcttcgat catcccggca aacggccgag
     5761 tcgttaattg ctttttattt ggatagtttt tatacagatt ttgcaaaaaa cgatgatcac
     5821 aaatcattcc gcaacagata aaagtcaaaa agtcaatctg ctttccgtcc acctgccgct
     5881 cgctgattgt ttgatcataa acgatgcgtt ctggcagatt gatggtttgc agcactacac
     5941 gatttggtag ctgcaaaatc gtccgctgcg gtgttaatgc tcggcttaga gaacggatcg
     6001 atacagggat catccatagt gatgaatcaa gggttgtatg tttttgaaaa aaacctgcca
     6061 gatccatgat cccttcattt ccaaatccga tgataaacat ggattgattt tcgggatagc
     6121 gtttgacgaa atcaagcagg ttctttaatt ctgttaaatg attgcattga gtgttggcac
     6181 aaatatacca gtcattgttt gcttgatttg caaataacgg gttgatctta tctgcaaaca
     6241 aatcataata gcgctgattc gttaaaaata agattggctg atcaatggaa agttctctta
     6301 gctgactgac tagcgtttct ccgtaaaaca ctttcgtcaa cgcatcttct acttgataat
     6361 caagttccat acgcatccct cctcttctta tttttatttt accattagta agcaaattct
     6421 agaaaagata agagcaaagc tcacctcata acttggaaat aaataaaaat taaataaaat
     6481 acatgtgttt tctatgtatg acatacggat tcggaatctc tgtctcttat ttattccttt
     6541 tctgctagga aataaaaaga aaagcacttg tagccctaga gccacaagcg ctttttcaac
     6601 tgtcttattt aacagcgtct tttaatgctt tacctggttt gaatgcaggt actttgctag
     6661 ctggaatttc gatttcttca ccagtttgtg ggttacgacc tttgcgagct gcgcgttcgc
     6721 gaacttcaaa gttaccaaaa ccgattaatt gaactttttc gccgttagct aaagcatctt
     6781 gaatagttga aaatacagca tctactgctg cagttgcatc ttttttagtt aaatcagtag
     6841 ctgaagcaac tttttcgatt aattctgctt tgtttgccat ggatagattc acctcctcca
     6901 caagagatat gatagaaata tcatttagtt aaaactttct gagtggtcca gaagtttaaa
     6961 aagaaatatt gattaacttc aatattctgt tatcacgata tcatatgaac cgcttaatag
     7021 caaggttttt accaattttc acatgctttt tttagtcata gaaacgtaac aaacgaaact
     7081 gtctttttct tttgaaaaaa aaacgtcgat atggtaaaat gcctattttc tcctacgagg
     7141 gataattcgg atggacgtcc cttcaaaagt aaatgctttt ctgatttgat tttctaagaa
     7201 acgttcataa gaaaaatgca tcaattcctc ttcattgaca aaaacgacaa atgtaggtgg
     7261 tttgacagcg acttgtgtag cataaaaaat cttcaggcgt tttcctttat ctgttggtgt
     7321 tggattgatt gctacagcat ccataatgat atcattcaac acagccgaag agatacgaag
     7381 attctggttc atacttactt gttcgatcat ttcaggcaga cgttccaacc gttgtttagt
     7441 cactgcagaa acaaaaacga ttggagcgta atcaagatac cggaattcat cacgaatctc
     7501 ttgttcgaaa tcacgcattg tattggtttc tttctttaca agatcccatt tattcacgac
     7561 gataataatt ccacgaccag cttcatgggc gtaacccgct actcgcttgt cttgttcccg
     7621 aatcccttct tcagcattca gtaccatcaa aacgatatca gaacggtcaa tggcacgcat
     7681 tgcacgcatc acgctgtatt tttcagtatt ttcgtatact tttccgcgtt tacgcatacc
     7741 agctgtatct atcattaaga atttctgacc actttcagaa acgaagtggg tgtcgattgc
     7801 gtctcgggtc gttccttcaa tatccgaaac aattacacgt tcttctccta aaatcgcatt
     7861 gatcaaagag gatttaccaa catttgggcg accaatcaaa ctaaacttaa tcgtgtcgtc
     7921 gtcctcttct tcagtctctt cagagaaata cttcactgct tcatctaaaa tatcaccaat
     7981 ccctagtcca tggcttcctg aaacaggata aggatcgcct agccctaagg aataaaattc
     8041 atagatatcg tttctcattt ctggattatc tactttattg actgctaaaa tcactggttt
     8101 attacttctg tataaaattt tcgcaaccat ctcatctgcg tctgtcacgc cttcgcgtcc
     8161 gctgactaca caaatgatta catctgcttc ttcaatcgcg atttctgctt gatgcttgat
     8221 ctgatccata aaaggttcat cgccaagatc aattccgcct gtgtcgatca cgctgaattc
     8281 tcgacctaac cattcacctt ttgcataaat acgatcacga gttactccag gagtatcttc
     8341 gacaatcgag atacgctcac cagcgatgcg gttaaaaatc gtagacttac cgacgttcgg
     8401 gcggccaacg attgcaattg ttggatttgc catattattt cctcctcaca agtttccaat
     8461 ctcacttagt ttaacgaatg cattgccaac tttcaagcat agattgtcaa ttaaacgaag
     8521 tatttttatt ccttattgaa atactaatcg cttttcaaaa gaaaaactga gacaaaagca
     8581 gctgctttca tctcagtcac agatacaaaa tcaattattt ttcagaatca tcggttgctt
     8641 tttcttctac ttcttctttc aacgcatcac ctaaaatgtc tcccatagta aatccagtat
     8701 tttcttctgg taattcataa gactcaacat tttttggttc ttcttctgaa gctggttttt
     8761 cttctaaagc tttgatactc aatgcaatac gatgttcttc tggatggatc tctaatactt
     8821 tcactttcac ttcgtcacct tcatgaagca cttcatgtgg tgtcgcaata tgtttatgtg
     8881 aaatttgaga gatatggact agcccttcta ctccagggaa cacttcaaca aaggcaccga
     8941 agcttgtcaa acgtttaact gttccgtcta atactgtgcc aactggagct tttgtatcga
     9001 tttctgacca aggtccaggc tgtgtgtctt tgattgacaa ggaaacacgt tcttgttcag
     9061 gattgataga caacactttg acatttacat catcattgac agccaataca tcacttggtt
     9121 ttgctacatg actatgtgaa atctctgaga catggactag accgtcaatt ccgcctaggt
     9181 cgacaaatgc gccaaaatca gtaagacgtg caacacgtcc actgagaaca tctccctcat
     9241 ggatagttga aagaatttct tttttctttt cttctctttc gttttccact accgctttat
     9301 gcgataagat caaacgattt tctgatggtt cgatttcgat gattttaaag ctcaatgttt
     9361 tgcctttata gtctgaaaag tctgcaacaa aatgatcttc caccattgat gcagggacaa
     9421 aaccacgtac accgacgtca acgactaaac cgcctttgac aacatttgta acaggtgctt
     9481 cgatgacctt gccctcttgg aaatctcttt cgatatcttc ccaaactttt ttagcatcta
     9541 aacggcgttt tgaaagtagg tagctgccgt tttctttatc tttgccgatc gtactgataa
     9601 cgactaaatc taatacatca ccgactttta ctacttcgct gatatcttct accggcaaag
     9661 ttgataattc ttttgccgga acgactcctt caactccagc cccttcgatt ccgacaatca
     9721 cttgtttgtc ttcaatgact aaaacttcgc ccttaacaac atcgccaact tttacttctt
     9781 gtacgctttt cattgcgtct tccattgact ggttgttctc ttgattttga tcaaattctg
     9841 tcataacact tgtcctccta accaacattc tgccgtaagt acggtactgt ttttagttta
     9901 attgattatt caaaaaaaat aaagcgattt cgttcgtttt ccttgaaaaa acaattgttt
     9961 ttcatttttt ttcttaattt aaaaaagaag ataacctttt ttcaaaacaa cagcttttat
    10021 tgctgcaacg acttctggaa tcgatttgcc agtcgtatcg atacgaacag catcctctgc
    10081 ttgcttcagc ggagagacgt cccttgttga atcataatgg tctctttcct gtatctcttt
    10141 cgtcagtgtt tcaagatcag tcggaattcc tttttcttgg ttctctttga aacgtctttc
    10201 tgcacgctca cttacgcttg ctactaaaaa gattttcact tctgcattcg gtaatacagc
    10261 tgtcccgatg tctcttccgt ccatgacaac gccaccgcgt tcccctattt ttcgttggac
    10321 agcaactaat tcttctctga cttttccatg tgcagagact tcagaaacag cttttgtgac
    10381 atctggttga cggatttcta aagtcacatc ttttgatgct atgaatacat gctgtccatc
    10441 ttcttgttgc gaaaaagaga taggatactt tttgatcagc gaaactagcc cttcttcatc
    10501 tgaaaaagat acattgttcg taatagctaa ataagttact gcgcgataca tggcaccggt
    10561 atctgtataa atataattca attcttttgc taatatcttg gcaacggtac ttttcccaga
    10621 tgaagcaggc ccgtcgattg caatactgat ttgtgtcatg atataactcc tttttctcag
    10681 aaaaaaagcc gcaaagcgcg gctattatta tttaattctt aattcttgac cagggtaaag
    10741 cataaagttg tttggatcta agccattcaa ttcaaacaat tgatcagtgg tgatccctgc
    10801 acgctcagcc acttgttttg gaccttcgcc tgcttgaaca gtcgttgttg aaccggctgc
    10861 agctgatgaa gaaggtgttg tttctgctgc attgctgctt gaatcccctg cttgataagt
    10921 tgcctcactt gaggaagtcg gtgttgtctc tgctgcaata ctgctttctg acgttacacc
    10981 tgtatcttga gacacagtag attcttccgc gattgtacta gattctactg ttgaagtctc
    11041 agttgactcc tcttttgttg tacttgtaga actttctact actgatgtcg aagtttcaga
    11101 agaagttgct gctgttgcat tgttgcttga tccatttctg atccagaaat aagcacctgc
    11161 tggaatagcg atacataaga ccagtaaaat caaaaatagc gaaagaaata atgtatttcc
    11221 tttcttttgt tgtctctgag aacttcttga tgaaacttca tcatctgtat cgtagattgg
    11281 ttgttcccat ggttcttgtg tttcgttatt ttctgtagat tgatgtctat cttttctgct
    11341 cactgtttta cctcctaaac tctatcgtgt gtattatacc ataggaaaaa gtgtcttgtc
    11401 ctaaaaaatc aatgttccat gtttttttta aataaattta gtaaaatcgt ttcccaatga
    11461 agttgttcag tcttagaaaa aagcttcctc tttggcagaa gttctgcttt tgctccatca
    11521 atatcacaac aattctgcgg cttttcggag agtttttcgc caaaataagc taagatgaat
    11581 tttctgcggc agttttcttc attaatatag cgaagcatct gctgcaggcg gaattctttt
    11641 tctttttcct gacgtttgag ccgttccacc caattttctg gtttttcaga ctgctggatc
    11701 agctccatcc attttttttg cagctcatca aagggagctt gctttgtggc atattccaaa
    11761 taaagctcaa aactttgtcg ctgttctcta gaaagttgat taaagaaata atggatacgt
    11821 tcatccccac tttgataaaa aagaattgct gcactttctt cctgatctct gcctgctcga
    11881 ccgatttctt gtacatagtt ttctagactg tctggcaaat cataatggac cacataacgg
    11941 atatcagatt tatcgatccc cataccaaat gcattcgtag caatgagaaa ctgcagctga
    12001 ttttttacaa actgctgttg aagttgtctt ctttgagctg catcaagacc accgtgatag
    12061 tatccaactg taaaacgttc cctgaataaa tgatacaatc gttcgacttc tttttttgtt
    12121 gcacaataaa tgattgccgc tccttgtgct ctttccatga agtgttcaag ttcttgttct
    12181 ttttgctgcg ttttccgaac aaaaagcgag atattttgcc gattgactga ttgtctgatc
    12241 tcttttggtg gtcgatcaaa tagtacatga cgtatatctc ttgctactaa atctgttgct
    12301 gtcgccgtta gtgccagtgt gaccgggttt cctaaaagct ttcggatttt tcctaactgt
    12361 tgatattctg gacggaaatc aactccccat tgagacacgc aatgagcctc atctactaca
    12421 tataacgcaa tattttgttt tttcagatgc tctaaaagag aaggattagt caacatttcg
    12481 ggactcaaaa acaaaaattt atattgcgat agatgacgga ggacgtatct tctttcagat
    12541 tgcgaaagta aactattgaa agcagctact cgtttctccc ctcgtttttg taattgcgtc
    12601 acttgatctt ccattagaga aagcagcggt gacacgatga tgaccagacc ttccattaaa
    12661 taccccgtca attgatagca aaggcttttt cccgttcctg taggcaaaat agcgagagta
    12721 tcttctccat caagcaacgc ctgaataatt tcttcttggc cgggacgaaa tgaatcaaat
    12781 ccaaaatatt tttttaattc atttattaac attctgtatc gcttcctttc tcaatagata
    12841 gatttgatac aaacgaaatt ctccataatc taattgtcca tctacattcc attcgctaaa
    12901 gcgccactct cgcacggaat ccttatttgg ttccaaacgt tcgattgttt ctgcagagag
    12961 aatccactcg tatggaaaat catcaaaaaa caaggcccac tcaataagat gatctgttac
    13021 tgttcctctt tttaaatgac ggattgccat gatctggtca attgtctgac cattcataaa
    13081 catctgcttg gtgatcatca tgctttgatt gaaattttgc tgtaataatg aatcgatcaa
    13141 agcgtgccaa agcgaatctg gccgtttttc gatttgtgct aacaaaaggt ggatacatct
    13201 gctttgttgc agataaacct ctgactcatc attgtttttt gccagctgat aaggaagcag
    13261 ccctgttcgc tcatttccag ataattggtt agctaaaaaa tctgctgctt cttctggtat
    13321 ctccgaaaac agctgcacca aactctcata cgcagtatca atcaaaattt cacgtggcaa
    13381 ttggctttca ctaagccatt tttttagttg gaaagtataa aaaggacttg tctctgctgg
    13441 aagatagtct tgaatacccg ttgccagatt agaaattact tgaacagcaa atttaatgag
    13501 ccgccaacta gtttcccctg ttctgccata gcggtcaaaa cgcaaaccag tatgttctat
    13561 ttgatgttcg gacaacatat cagcccctgc aggcgttagc tttgcttcat tttcatttat
    13621 ttcgatccaa ccttgctgcg caattttttg caaaatttga taaaacttat cctgcttcaa
    13681 ttctggaaaa ctgttatgga taaacaaaag ttcgtgggta aatccataaa taagtactga
    13741 agtcgttcgt tttcccacaa ggagttggta aagagaacta atccgcagct tgtgtcctga
    13801 tgaaaatagc gataaaataa aaacaatcat attattctcc ttaagttagg atggttagaa
    13861 atgtcactac tatgtaaact tgtacctgaa cgctgtattg catgcggtct ttgtcaaatc
    13921 gaagcgccag atattttcga ttatgatgaa gaaggaatcg ttttatttgc ccaagaacca
    13981 gaggctcatc aacagttcgt gatgccggaa gaagaaacat atgtaaaaaa agcgtatcaa
    14041 aaatgtccag ttcgagcgat tttattagaa aataaaatat aaatataaac gaaactttaa
    14101 aaacgtacac taagttctct aaacttgaaa ataagccggg atctgtttct cttgttccgt
    14161 gaaatcccga cttattttga aagattcatt tgctatctat agttaattga ctagtttttt
    14221 cacggaccct ataggaccca tttatattat ttcaactttt tcagccagtt ttccaatgta
    14281 ttgacgatca cagtttgctg gtctgcgtta gaaatttctg cctctccatc tcctttttgc
    14341 tcaccatagc tgccaaaacc tccatgattt ccgccattga ttgatacata ctctgttctt
    14401 tccggtaaat actgcttagc ttcttgataa gcttcctgat tcaatacttt atcttttgtc
    14461 gccgtgatcg ataaaacagg aacagctgat tctgtcaatg ttcctttttt gtcgggataa
    14521 cttgccatga agaatacccc ttttaaatgt ttgtctgaat gattcttatt gacataacga
    14581 cttgccatta cgccacctaa tgaatggcca ccgatcacat aatcggaatt tggaaaatct
    14641 tctttgacct tatccgcttt gtttccgccc atcactgcta gatcaagtgg cattttgaca
    14701 atagcgactg aaaagccgtt ttttgctaaa tcttgcgccc aaatactata actttctggc
    14761 tctacgagag caccaggata aaaaataagt aatggagact ttccatgatt ctttttctct
    14821 ccttcaaaaa ataagtaatc ttctttgttt tttccttctt ttaccaacac ttgcgcttct
    14881 gacgatggtt cgtacgtatt ttgctgtata tagtaataac cggctccacc gagtaatcct
    14941 aaaatgatga ctaagccaat tagtattcgt ctaaatattt tcactttctc ccaccttttc
    15001 tatctgagtg accccttttt cttaatcaag tatgattata ccttaaaaaa ataagagaga
    15061 aatgcaaact ttccagcatc aaataaaaaa agacggaata tccatttgaa tatccgccct
    15121 ttttgtatgt ttatttttat ttagcacata aacgatacac agcttctgca taaatttcca
    15181 ttgctttata catactactt aattcccatc gttcattcgc ctggtgcata aagtcaggag
    15241 tatcagggaa catcgcaccg aatgccacac actggttcat cgtacgagca aaagtagcgc
    15301 cgcctgatac aaacggttca gtcatgtctc cagtaatatc gcgatatgtc cccaatagat
    15361 ttttcactaa ctcactgtct agtggcacgt ataatgaatc aaggaagtca aattcttcat
    15421 aagtcaaccc atatttttct gctgttttag ttaatttagc tactaaatca tctttcttga
    15481 atgtaacagg gattctcata tctacaccga tttttgtttg ttctggtgtg atttccaaac
    15541 ttgcaaagtt catcgttagc tcgccggatt gttcatcttc tgttttgccc acgacagctt
    15601 caccagttgc attttcttga actaattttc ctaagaaatt gattgggcca aaatcaaata
    15661 cttctgaaag agcaattgcc agacgagaaa tagcattcac tccttgtgct gcatctttcg
    15721 cgtggatact tttaccaaga acgacgatgc cttcaccttg ttcttcgaaa tcgaattcgt
    15781 gttttttcaa tgcttctttt acttcagaaa gcttttctcc agagtaagga gctgcatcag
    15841 gaacaacatt caacgctcct ccagctttta cagaaaactc gttcgttcct ggtcctgtca
    15901 agtaagcttg caataagcct ttttctgcat aaattaaagg aaattcagca tctggcgcaa
    15961 atccttgcgt aattccttct tctttttcgt tatatttttc taaacaacgc cataaatttt
    16021 cttcatctgt accaaagata aaacgaatac gtgtcttgaa ttccacgcca gcatcaatca
    16081 aagctttgac ggcatacatt gccgcaatag aaggaccttt gtcatcttgg gatccgcgac
    16141 caactaacca gccgtctttt atagttggat cgaatggttt tgtttcccaa ttattttcgt
    16201 cccctgctgg aacaacgtcc atatgacaca agataccgaa taattcatct ccagagccaa
    16261 tctctgaata accgtaatag ccttctggat ctttaaatgt tctaaatcct agcttttcac
    16321 tgatttccaa tactttatcc aaagcttcaa tcactttttt accgaaggga tgtccttgtc
    16381 ctgaatctgc ttcatctagt acggaaggaa ttcggatcaa ttcgctcaat gattctaatg
    16441 cttgttcatg atgttgttct gtcacaaatt ttttcatgat attatcctcc taatatgtta
    16501 aaaagggtca tgtcaaattc ctccgacaca acctcttctt ctcaaataat gacgttttaa
    16561 gcctgctcca tcagcaagca gaatgttctt aacaaatgat gaaagaaaaa acactcactg
    16621 aatggagcta ttgacttgtc cacgtgctta tcgatagccg tttatcggtt tcttcattct
    16681 tatgatagac cttgtacctg tatttgtcta ggtttagaac attgctgcta cacctaggat
    16741 cacacagatg gctacaaata atatcgcaat taatttaccg gtaaatttcc accacactga
    16801 aacatcgaaa cgagcaattg ctacagcacc cattacgata gcagatgttg gtgtaattaa
    16861 gtttactaaa ccagatgcag attgataagc agtgattact aaatccttac cgacgccggc
    16921 aaattcaccc ataggtccca tgattcccat tgttgcagca gctaagccgg aagttgaagg
    16981 aatcaagaat gacataggga tatagaaaat aaatgtaata atgatgaata cacttgaaga
    17041 taatgaactt aatccttgtt cgccccaatg taagatggtg tctgtgatta atccattgtt
    17101 cataaccact tggataccac gagcaacagc tactacgatt gctacaccga tcaaatcaga
    17161 agcaccagct aaaaattcag agacaaattc agactctttc atgccataag tgattgctac
    17221 tacgatggac attactaaga ataacatagt aatttctggg aagtaccagt cacctaatgg
    17281 aagcatgctt ttacctagaa ccgtacctag taccggaagg tttgttaacc agtttgtcaa
    17341 tgattcaaaa atcgtgaaat tcgcattcaa ggttgtccaa ggaatcaaac tgatgatcat
    17401 gataccaaaa gtaataaaga acaatacatt aacatgtttt tgttttttcg tgattacttc
    17461 ttgacgaccg attgcttcaa tatcgaaatg cttcatatct tctttacgtt ggttgtatac
    17521 taatgatttt gttggatctt tttcgatctt tgatgcataa cgatacacat atacaataga
    17581 aatagctaac aaaataacga acatcaagaa tcgaagacca ataccttcac ccatcgaaat
    17641 cccaacagct tgtgaagcta cgcctgttgc aaaaggattt accgttgaag ctagacagcc
    17701 tacttgagac cctactaata cgattgctac agctactact gtatcaaagc ctacagccat
    17761 catgactggg atcagtaaag catagaatgg aatcgtttct tctgccattc cgtaagtaga
    17821 gccgcctaat gcaaataaca gcatcaatac tggaataagc attttttctt ttcctttgaa
    17881 tttcttcaca actgaagcaa tcccggcatc tagtgcccct gttttgttga taatacctaa
    17941 aaatccacca acaaccaaaa tgaagaaaga gattgggatt gcagcaggtg tttctttagt
    18001 tagctccccc gctggtacac ctagcatccc attgattggc gccatgaaaa tatcccacag
    18061 cccttgagga ttttgttgta ctcgttcata tgatccagcg acgatgttgc ctgcatcgtc
    18121 tacactatag tgcccagctg gtataaacca agtaagtaca gcgattactg caataataat
    18181 aaataagacc gtatacgctg aaggcatttg aaattttttc ttttttgttt gttccatctt
    18241 cttcttcctt tcttttccgt tagagacaac aattttggct ctaatgttat tttatcaaaa
    18301 agagtatcag aacaaaaata ataacacgca ttttgtaaaa cttttgttca aaaagttcgt
    18361 ttttccctat tccctcctct tttttcagtt ttttaaagct ttcttctttt tcattgtagc
    18421 gtttcctttt ttttataaac aaacattttc gtcttttaaa aggagaagaa tttcacattt
    18481 gcaaagaatt gattgatttt tatgcataaa tcaaaaaaat actcaaaaca aacaaataaa
    18541 acgtttacat tattgcatgt cttgtatttt tttccaaaaa aaaagaccaa gaaatttctt
    18601 gatctttttg atatttattt gactgctgta tgacgttgtt cgaactgatg ttgttttcga
    18661 cttaaccatg gaagaagttg cgcatgcagc actaaaaaga cagcactcac gatgaatcct
    18721 ttgatcaagt tgaaaggaac gatcccaatc agtacatagt ttgctaaaga catgcccagc
    18781 atctgtgttg cgttcagccc taaaaacatt aagtaaagag gcgtaataac gaaataattt
    18841 gcgatactca taaaaatagt tagagcgatg gttcctgtaa tgacacctaa aaatttgttt
    18901 cttcctttat gctgtttctt tacactaaag aaataataaa tcgggaatgt aaagatcgtt
    18961 gttgataaga aactagcagt gtctcctacc aaattatccg gagaaaaacc tgtagtcagc
    19021 agatgtaata ctgaacgcaa gaacgctgtt gcaactccgg ccgctggacc gaataagaac
    19081 atgctgatca ataccgagat atcactaaaa tcgatcttca aaaaactaaa agccggcatg
    19141 atcggaaaag caaaatactg taaaactaat gcgatcgcag caaacatcgc tactgcaacc
    19201 attttctgta cacgggtgtt cttcatttga aatcctcctg ttaggactcc tcttcagcga
    19261 actttttgac gtttctctcc aaatgaaaaa actctatttt ccagggaaaa tagagttaga
    19321 gttatttgat caagcgtcgt caaataagct ctatcttctt tatccagact atactgtcgg
    19381 tatcggaatt tcaccgattc agctattttt ttgcaaaata ggtcgtggac tataaccacc
    19441 ggtcgggaat ttcaccctgc ccctgaagac gaaccttata taatttttgt ttacatattc
    19501 attatacata tctattcaaa aaacgcaaca tctaacaatt ataaaaagta agtgttcatt
    19561 ttgagtggtt cagcaaactg ctgacttctt ttttgttcag ttcacggtaa tccccaggac
    19621 gtaggccttg taaagtcaga tctccgtatt tttcacgttt tagtttttgc acaggaagac
    19681 caactgcttg aagcatattt ttcacttgat gattacgtcc ttcgtggatc gtcaattcca
    19741 cgatactgtg gtttgtctta ggatccactg ataaaatttg gaatttagct ggagatgttt
    19801 ttcgtccttc gatctttatt ccttttgtca aaggtgcaag catcgatttt gtagcgatcc
    19861 cttttacctt tgcaacgtaa actttatcca cttcatgttt tggatgtgtc aggcgctgag
    19921 caaaatctcc atcatttgtc agtagcaaga tccccgaagt atcgtaatcc agtcgaccta
    19981 caggatagat acgttctttt accatcggca aaagatccgt aacaactttt cttcctttgt
    20041 catctgcaac agcagaaata actcctttag gtttgtacaa taaatagtaa ccgtattctt
    20101 cttgataaat tgggacttca tccacttcca cagtgtcatg tttcccaact tttatcccta
    20161 attcccttac aacttgtccg tttactttta cacgtcctgc aaggatgaat tcttctgctt
    20221 ttcgacgaga agtgatgcca gcgtgggcaa ttactttctg caatctctcc attacaatta
    20281 ttctccttta ttcattccat caaagaatag atctggtgac acgtcttctt caagtgaaat
    20341 ttccatttct tgtgtatcag gcagttcttc taatgatttc aaaccaaaat aatctaagaa
    20401 gtagccagtt gttccatata atatcggccg acctggtcct tctactctac ctttttcttc
    20461 aatcagttta tgggcagcta gacgctgtac cgctcctgaa acctgaactc ctcgaatctg
    20521 ctcgatttca gctcttgtga ttggctgttt ataagcgatg attgctaagg tttcgacggc
    20581 ggcttgagat aatgtgtttg atgtcccttg cgcatatcgc ttaagtaagg gagccagctc
    20641 ttttttagtc gttaatacat aatggctttc cgtttcaaaa atatgcaaag ctgagtgcga
    20701 gttttgagtg taagattctt ttaattcttt cactaaactt gtgacttctg cttcgctgag
    20761 atttaacagt ccgcttaatt cttcattact gatcccttcc tcgccaacaa taaaaagaag
    20821 ggcttcaagt tcacttaatt tcgtcatgtt cggtatctcc tactgctgga taaagcataa
    20881 ttgattcata gttttctttt tgttgcgcaa tgatctgccc ttttttcatc aattctagca
    20941 aagccataaa agtagtgaca acttcacttt tagaaggtga ctcaaaaaaa gaatcgaaag
    21001 tacagccatg cgcatttctt tttatacgct cttcgataaa taccattcgt tcttcgatcg
    21061 ttgtgtcatc gggcgtgatc gttttttcca ctttttgaga aaatttctta cgttctaaga
    21121 ttttctgcat ggcacggatc aaatcgattt ttttgacttg tcctcgcttc aacggctttt
    21181 cagcatccgc taacccatcg atgttcatag gttcttttgt gtaataaaga cttcgttcct
    21241 ctgctttttc tgtcagtctt tcagctgcat atttatattt acggtattct aaaagctgag
    21301 tgaccaattc ttctcgagga tcttcttccc ataattcttc atcgtctgtg ccgaattctt
    21361 ggacaggaag caatgtcttg cttttgattg ccatcaatgt agcagccatc actaagtatt
    21421 ctcctgcaac agctagctcg agtgttttca ttgtatggat ataattcata tattgctccg
    21481 tgatcgtagc gatcggtata tcatatatat cgatttccat ttgctgaatc agatgaagca
    21541 gcagatcgag aggaccctcg aatacatcta atttaatatt gatctccgtc aactcattca
    21601 ctctttcttc tttttctttg ctagccattt tccaatgtaa ggctctgtga tttcagttgc
    21661 acggatggag acatccgggt acttttcctg taattcatct aacatttgaa agcccatatt
    21721 ctcaccgcga taagacggat tcaaagaaat gtcatggatg atcagctgat cgttcgttgc
    21781 ccagtaaata ccaataatcc cttgaatgtt ggttgaacct tctggcgtaa agaaaaaaag
    21841 ttcataatcg tcttcagatt cgtacgtatc aatctccttc aacaaagaag ttttacctgt
    21901 cagtttctta tggaaactca atagtcccat agcaatttta cggttttctt tccggtaatg
    21961 tgttaacatc ttctcacctt cctatgacta agcccttgga aaatgctgct tataaacatc
    22021 cgtcattctt ttcttcgtga tatgtgtata aatctgagtc gtagagatat ccgcgtgacc
    22081 taacaattcc tgtactgtcc gcaaatcagc tccattttct agcagatgtg tggcaaagct
    22141 atgccgcagc gtatgtggtg ttacttcttt gtaaatccca gcctctcgta ctaactgctt
    22201 cagatttttc cagattcctt gccgagaaag acctttccca tgattattga caaatacatg
    22261 catttcagat gcgtccttta caagtaatgg acgtgcttcg tctaagtagc gttccagcca
    22321 ttggatcgca taatcaccta aaggaatgat ccgctcttta tctccttttc caactgtctg
    22381 taccagtcca agagaaagat gaagatctcc caatttcaat ccaatcaatt cactgacgcg
    22441 catcccagta gcatacatga cttccaagat tgcacgatct cttatcccta acgtttttgt
    22501 cgtatcaggt gcttcaatca gcttttctac ttctgtcaga gtcaatgtac ttggtaattt
    22561 ctgtgctttc ttcggcgtat cgatatgttg catcggatca tgatcagtga tacgctcttg
    22621 ccgtaaaaac tgatggaatc ttctgagact tgagatcatc cgagtaacag ttgctgtcgc
    22681 ttttttttct tcatgcaact tctccagaaa acggatgact aaaaagcgat cgacatcctg
    22741 ccatgaagca attttctgct cctgtagaaa atgcaagtac tgttccaaat cacgttcata
    22801 gctttgtcgt gtattggcgg acaatccccg ttcaatattc aagtagtgga gataatcgtt
    22861 gatttgctct ttcataataa tccccctcat gcctctctat catatcagat accgcttcat
    22921 tctgatagtc tttttccata atctgagcaa agaaaaagcc gtctagacac tcagacggac
    22981 tggtttcttt tttcggctct ataatttatg ggactgatga atcagaattg attcttcagc
    23041 cccattttca ttttaggtat taaa
//
LOCUS       DARBET010000038        22031 bp    DNA     linear   BCT 07-NOV-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4027
            SAMEA114569841-rid20112183.denovo.038, whole genome shotgun
            sequence.
ACCESSION   DARBET010000038 DARBET010000000
VERSION     DARBET010000038.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA114569841
            Sequence Read Archive: ERR12203666
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 22031)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 22031)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001972618.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001972618.1
            Genome Coverage       :: 132x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/07/2023 13:18:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,041
            CDSs (total)                      :: 2,975
            Genes (coding)                    :: 2,908
            CDSs (with protein)               :: 2,908
            Genes (RNA)                       :: 66
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 67
            CDSs (without protein)            :: 67
            Pseudo Genes (ambiguous residues) :: 0 of 67
            Pseudo Genes (frameshifted)       :: 26 of 67
            Pseudo Genes (incomplete)         :: 28 of 67
            Pseudo Genes (internal stop)      :: 20 of 67
            Pseudo Genes (multiple problems)  :: 6 of 67
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..22031
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA114569841-rid20112183.denovo.038"
                     /isolate="VRE4027"
                     /db_xref="taxon:1352"
                     /country="Denmark"
     gene            367..555
                     /locus_tag="SCJ86_001653"
     CDS             367..555
                     /locus_tag="SCJ86_001653"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016627820.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEG1169194.1"
                     /translation="MELIVFTNNGQTYHFYEVENFKPTTTGFSFIYTGKATGVTRSAT
                     FNNTSTAGYALAEVQNES"
     gene            545..805
                     /locus_tag="SCJ86_001654"
     CDS             545..805
                     /locus_tag="SCJ86_001654"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016627821.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEG1169195.1"
                     /translation="MNHEKFIEKCKAIVRERIENEIADLSGAVPEFSIFIVWSCKTLQ
                     NSKALVSASLKGAPYFEITLNGDKGEIYVDTYLKKSNECIKV"
     gene            846..1073
                     /locus_tag="SCJ86_001655"
     CDS             846..1073
                     /locus_tag="SCJ86_001655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002370649.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEG1169196.1"
                     /translation="MRNYWYVSLTNEYPRTIGDCSVRVVRSVQIKGKYSIVEMLREAT
                     PNEVDKCKLIYCGHGYWKDEYIQYNIERYIK"
     gene            1138..1710
                     /locus_tag="SCJ86_001656"
     CDS             1138..1710
                     /locus_tag="SCJ86_001656"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002371779.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEG1169197.1"
                     /translation="MKLKDYIREGYNVVTAPNLAYKIQEDYPNALVFTDRALDAIPIG
                     KLLVDRFYSNNPAVLSAKPLQNAYAIERFTCEFTGYETVVPMTGETGRKVIQQIKKRI
                     AELTLDDGSNTNLLEIKLRDTDSVPEVYYKGQRMDEMPIGLVDITYHWDTEEYECQVP
                     KGANDITIEYYDSHNNKYLDRKIIGHKRDM"
     gene            1711..2022
                     /locus_tag="SCJ86_001657"
     CDS             1711..2022
                     /locus_tag="SCJ86_001657"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002358084.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HNH endonuclease"
                     /protein_id="HEG1169198.1"
                     /translation="MIDVSSKQARARFYGSTEWRKLRSQCLERDHYECQWCKAEGRLT
                     TQHDSILEVDHIKELEYHPDLALDINNLRTLCKDCHNKRHNRMKYRGQQKKRKWDDEW
                     W"
     gene            2013..2273
                     /locus_tag="SCJ86_001658"
     CDS             2013..2273
                     /locus_tag="SCJ86_001658"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002371775.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEG1169199.1"
                     /translation="MVVRKTANRDDVKRAEAIAMVDKAIDKLVLFKSQLIEGTTTVED
                     ISINNRLIGNANEVTGTFNINVDIDYTLSPFAHIVESNEQDG"
     gene            2434..2793
                     /locus_tag="SCJ86_001659"
     CDS             2434..2793
                     /locus_tag="SCJ86_001659"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338886.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEG1169200.1"
                     /translation="MADLQERNRLVSAEERRLNKLFKDIDKDKKKVVSGLITQAARLK
                     ILLDEMWIDISEKGDYELFSQSENQKPYERERPVAKQYNSRDQAYQRVIKQLTDYLPE
                     EKREPTKNAALDGSDLL"
     gene            2790..4433
                     /locus_tag="SCJ86_001660"
     CDS             2790..4433
                     /locus_tag="SCJ86_001660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338885.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="terminase large subunit"
                     /protein_id="HEG1169201.1"
                     /translation="MTTLKPFYFDEYVDLYEKGVIPFNKERIQLITYLKNEVLVRDDI
                     YFDEEMIQKFIKYTEKNFFPLAKYQKFITPFIFCYQKEDDEVFFDEILNSIARGGGKN
                     GFMSARDSFFISPLYGVRNYDVTITANSEKQGKVSFKEVYEMVQMRKLEKQFYLTKMA
                     IVNNVTNSIFSYRTNNPKTMDSARDGCLEFDEIHQFESSDLVDIQRSGLGKIKHPRTF
                     YNGTNGHVREGFYDKMLERAQKIFSGENKNDRLFPFICKLDNIDEMDNPKMWSKANPM
                     FEEDSAYSKRLFSTVMKEYKKLEDEPSGRREFVVKRMNFTEGDAESDITTHEKLMATK
                     QELPNLNGRSCVAGFDYASIRDFATVGLLFKIDDKFIWQQHSFARKKFLDTFKLKAPI
                     KDWEKKGLVTIVDEPSIDPRHLIDWLNEKRKIHQIEIVCADGFRMDLLKPLLEKESYN
                     FEFIRNIRGVQSKVAPIIEDGFANERFIFGDDPMMRWYTNNSFIKEDKLGNKTFLKKE
                     PVRRKTDGFHAFLAALYKREEITDMDYEEAFDMLDEIEF"
     gene            4468..5550
                     /locus_tag="SCJ86_001661"
     CDS             4468..5550
                     /locus_tag="SCJ86_001661"
                     /inference="COORDINATES: protein motif:HMM:NF028182.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEG1169202.1"
                     /translation="MKKRYFLFVTIFIIIAICVLTVFLPQITEYLNYRIFTTKVDDSV
                     TSLSRLEFIKICASVFGPILTVMVFVNTLNMQKKTEQKQNELEVKQAKAENERAKKEL
                     LTQIDREFYFLLNMFIEIQKDQNAKQAVIALQKLAIFNEKGFNQDYAFTSKDLSDSEI
                     GSTIEQSGPLKGTVEYIKFETFDVSKSMGNYQTKTKDVATSLHDQKVAILNEQYEKMQ
                     IYLGRYFKMFHRIVKTLNEYYDDYNDFDVKRYTKYIGTLRTQISPAEFQVILFNSLYI
                     KRGFGLGIQLIGSGFFGDDFDFETNQHFETSINEQWFLSLSTVDSDNSIKRQKLSEYI
                     KELGSVEYKKIANFESLYKLFNETKL"
     gene            5594..6766
                     /locus_tag="SCJ86_001662"
     CDS             5594..6766
                     /locus_tag="SCJ86_001662"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002371768.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage portal protein"
                     /protein_id="HEG1169203.1"
                     /translation="MSLFDIFKQSIRNEEPSEWIPDFVYGEEVSARAYLKIMAKNTVL
                     DFVARTMSTLEVKFKNKDGTSDWDYILNVRPNSDMSAATFWQKFFYRLLDDNEVLVIF
                     TDDNQLLIADDFSRTEYAVYDDVFTNVMVKNFVFERSWNMSDVIYMEYNNEELERFTR
                     GLFEDYAALFGRILEVAMRNNQIRGSVSIDSTGTANDEKGKDGKTRSQRLQEYIDRIW
                     NSFKTNSVAIVPKVKGFEYEEYTNKQGVSNQSLDELNKMKSSLIDDVANAIGVPTALI
                     YGEKAELDSNIKAFRKLCIAPLVKKLQDELVAKIISRQEYKNGERIEVKKVLQKDLLE
                     YATQIDKITSGGAFYIDEVRDEVDYDELPNNEGKIRIRTKNYEETVKGGENDNAES"
     gene            6753..7457
                     /locus_tag="SCJ86_001663"
     CDS             6753..7457
                     /locus_tag="SCJ86_001663"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010749273.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Clp protease ClpP"
                     /protein_id="HEG1169204.1"
                     /translation="MPKAKKVPFQFTNEIQNGKHILTLSGNVQKKYWRDDDVINAKDI
                     RDSLDTVTDDIVIKLNSPGGDVFEGIEIYNYLKDHPSNITVEVTGLAASAATFIVAGA
                     NDVIMNVGTSLMIHEASTFAWGNKQDIQKTLNALETIDDSILAIYSDKTGQSVDKLRE
                     WMNEEKWFTADEAVKYGFANSVKREEPQNEPQNIAAMIQDAVAVAIANLKEPVTNKIE
                     QESKQKSLIARLRKGE"
     gene            7462..8622
                     /locus_tag="SCJ86_001664"
     CDS             7462..8622
                     /locus_tag="SCJ86_001664"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002371765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage major capsid protein"
                     /protein_id="HEG1169205.1"
                     /translation="MLKITDKTADAKKAFNAISSKEDATPEQINAALEEYVTAIAEDA
                     GKQVRAEYEELKNVTDNRVLEARGIPTLTAEETKFYNEAVKSGGFDSDVVWPETILER
                     VFENLQNDHPILGLINFTPTVGRVKVIRARRKGVAVFGPLHKDLEGQLDAEFGATEFV
                     QLALTAFFLISNDTLDLGPRWIDRFINLSLSEAVRDVWAKKIITGSGKDEPIGLLKDL
                     DGAVTSGVYPDKASAGTLTFAKEKIVLELAGVMKKLSKYTYKVDGDDEGEVKYRSVAG
                     RVYLIVNPVDYWDIVARVTFANLNNVYGTTMPFIAVDHIIESVDVPESKCIAYVDKEY
                     EATQSRAEKVYVYKETFAMKRATLYAVDMLGNGYPTNNDAAQVYDLDFTAGE"
     gene            8698..8976
                     /locus_tag="SCJ86_001665"
     CDS             8698..8976
                     /locus_tag="SCJ86_001665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736767.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage gp6-like head-tail connector protein"
                     /protein_id="HEG1169206.1"
                     /translation="MNTEEFIKEYKARFRIFHTSEDEDIGKQLESGFADIKSLIGSFD
                     PSTYEKGKELVYERTRYLRNESLEYFYDNFQTMIMDASIDLAGAGYAN"
     gene            8957..9328
                     /locus_tag="SCJ86_001666"
     CDS             8957..9328
                     /locus_tag="SCJ86_001666"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002380685.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage head-tail adapter protein"
                     /protein_id="HEG1169207.1"
                     /translation="MLDMPIKSNYKKPKIVAGDLNTPVTFFEFQPNKGPEPGDEEKKE
                     LYFCTALVYNPSMKDRDILGTKEGVTIKIRDPHQSYIPTNKHKVRILDYRYGDKVWEI
                     IDVSPDLEENSIVKIVLELIT"
     gene            9344..9748
                     /locus_tag="SCJ86_001667"
     CDS             9344..9748
                     /locus_tag="SCJ86_001667"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337486.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEG1169208.1"
                     /translation="MSNVKFEGVQETIQALEKKYGEKKTKTLTKKAINVGAEKVEKQL
                     QTDMTVFKDKGYTIAEVVRKNATYRDYKAEAEIGWNGPHQRYRIIHLNEWGYTRKGKQ
                     IRPRGFGVITKSLKNSELLYFDAVGEEVKKSL"
     gene            9745..10155
                     /locus_tag="SCJ86_001668"
     CDS             9745..10155
                     /locus_tag="SCJ86_001668"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318063.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEG1169209.1"
                     /translation="MKDMLNIIYEALCLNEYIHSMTYNENTEQYRIKFYEQPETADKT
                     GPMITIRPVDTPNEAYHGSNKELSVEHMIQIDVESKYRATCKQIQYEIKKEMKKLGFG
                     QISGQGLDEYFTETNRFVDARRYEGNTKIYDTEY"
     gene            10216..10803
                     /locus_tag="SCJ86_001669"
     CDS             10216..10803
                     /locus_tag="SCJ86_001669"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002371756.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HEG1169210.1"
                     /translation="MTLVGFKKMTIGVFDSTGKIPAANLYVIEGEQNKGATVSAEISG
                     LSKEPSKVYGSNIAYYVSQKGTGDVSATFGLLDLPTEVNDKILGYKTDTNKISFLGED
                     TEPPYCAILMESEDLNGDTAMLAMFKGKFSRESINLNTTTNEAFEPEAEEYVFSAIAN
                     DVEGDAKGQTVAKYVGDEEASITGLKEMVFPAAGE"
     gene            10876..11343
                     /locus_tag="SCJ86_001670"
     CDS             10876..11343
                     /locus_tag="SCJ86_001670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631246.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Ig domain-containing protein"
                     /protein_id="HEG1169211.1"
                     /translation="MPDTFKIYKKNGTEFTKVAEGESPLSITGIAANTQVAKGDYQAT
                     RVVGGQESVKVDIPAFTTLPISVTGVTLDKTTAEVEQGGTLKLTPTVTPANATDKTGS
                     WSSSNTAVATVTGGNVTVKTDAEVGGTTEVSFTTTDGGKVAKCTITVIEKAAG"
     gene            11389..11697
                     /locus_tag="SCJ86_001671"
     CDS             11389..11697
                     /locus_tag="SCJ86_001671"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337251.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEG1169212.1"
                     /translation="MAKVRIELKEESGKKVYENIDTTGKDYRKALETIKKLNEDGVMI
                     WNRLDIYLDFAVGIFRDSKLTAEQILDGLPSEKVMSTLDDILGEVMGIESNPDPDAKK
                     "
     gene            11892..18626
                     /locus_tag="SCJ86_001672"
     CDS             11892..18626
                     /locus_tag="SCJ86_001672"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318068.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail tape measure protein"
                     /protein_id="HEG1169213.1"
                     /translation="MANGKPLGNMIIKLDLDSSAFSKGLAGAKNAVNHQMKAMKSQMQ
                     VMNSSGNSLGALQAKYNGLGGVLQANEKQVELLTKAYKDSFDSNGNATASTAKYANQL
                     NQATARSASYESQMKTTVGQIARMKVETEGFTGKLKANSDVLINSGKKIEAFGKGVSG
                     IGSALTVGVTAPIVAGATAVTSAAISWESAFAGVKKTNDEVVDSNGNVVYSYSDLEKG
                     LRNLATQLPGSHQEIANVAEAAGQLGIKTENVVSFTKTMIDMGESTNMSAETAATSLA
                     RFANITQMSQKDFDKLGSVIVDLGNNFATTESEITEMGLRLAGAGKQIGMSQGEILGF
                     ATALSSVGVEAEAGGSAFSKVMIQMQLAVEKGFGAFDQLKQMAEEQGVPWVNLVNAVR
                     DGGKSLKAVSEQMGFTSSDLKKMYKEADNSKSSLENFANVAGVTSEQFQKLFKSDPSE
                     AIIKFIQGLKDSEKHGTSAIKVLDDMDIKEVRLRDSLLRAANASDIFKGAIEKGNGAW
                     KQNTALTNEANKRYETTESKLKILKNEVIDTAIDLGGPFVDALRDGLNVSKPLIKNLG
                     NMAKAFSAMSDDQQRNIIKWVGLAAAAGPALKLLGTGTSVIGKVTSTTGRLTKSLVDL
                     AAKAAEKKAMDAFASSVTTAGAAAAKTAGAGGIGGLVPMMGKTASAASTAAGASGIGA
                     MTTSLGLLGPALLGIVGVGGALAVGYGAWKLFGEEAWNSSQRVKQWGTDVGEEIDSTL
                     DGVQKNIEGANGQFGLLKEGFTVDDANNMAANFATAGTSLETSLTGRIKALDDALKGL
                     PQSVQDAMKEVAESEEETLGQSLSKIKDNNEEIKKIRERAANQNRDLTVSELQQIQDL
                     MKGTSQAYVDTLDVSAKDRKKILSAMNGDVAQATEEEAKTWLQSLAKQKAATTENYRQ
                     MLEEKKAALKEKGVSKEFIDALEDEMNDYIKTTNQGFDSQIAAITNKYPQLINEVSLS
                     NGQLFSKTAAAMDNTGQYTKVFLESNEKILAQAKKTSDEIADNAEKNAEQLGMVADEA
                     THAGSTWNGLVLDEKTGKVKTNVQEVVNEATKDFTKWNELKPVVHDAKLTTNAKDVIS
                     IAAVQNGYWDDMEWKEKEALLEDECSKNVYKALENAGKWNELDIPSKEAILTSNTPEV
                     MGETLMNLGLWDTYKLQVKDLDLNAYKVYDAIDGSEEKLNSWAQLDNPTKELLLDNSD
                     FSTKLFNSEEMFKRWNQLSSNAKFLSANNTKFTEVLLSSDRMWNQWVNLPDNEKRLLA
                     DNEDLATTVLSSEEIYGRWTQLPDNQKYMLADNTDLANKIFASEESWNAWKAIPDTVK
                     NLLGNNIDLLAKVKDGTVSVEEYNKVMPLLKTLYGDNVNLKEAISGAKSSIENYNKNI
                     FPSDKTAVGHDEASQAAKDAFEAFNIFQTKIPDKITKTVSADFIGPMPKNAKGTNFHP
                     GGAAMVNDQKGPTYEELITLPSGETFIPKGRNVVLDLPRGSKVLNATKTKRLVPKYAD
                     GIGNITTVSSTLNLDALILAINELTTVLRTQLMAGTTDAKDTKLSDETKVADPIIPDS
                     LSEKSDQYLGIGAQWLTNLMNGWNSAVPQYMNSEMVFITNYLNALKLQNNPNYTQGAT
                     WNKNLLNGWNSLTGTFIATINSFCNQAMVTLRNYNTPMYNNGRTWQQNNLNGWNSLYG
                     SFIARVNQLGNDSINNLRSKSGGFYNAGTFLLQSLINGMNSLGNSLSTTMNNVANTMV
                     GGIGKGVNGVISGVNYVLKEVESSKNIGNWPIPQYAKGTDGHPGGLAMINDQKGLVHE
                     EYVQMPDGRSFIAKGRDLLVNLPKGAQVLNASLTKKLKERLNIPHYEKGIGNLDIVDL
                     LDDEKRMLEFLTSKVDFSGINEPWLDMTKSGTSLMSKAANTMLQSKLSEFFTHGNFDG
                     AVNANGVYQYLVDVAQKVMGKFPGLTVTSGYRAGDAYYHGKRQAIDLAYPGISGDPRY
                     TAAANYAFEKFPSKIAYVITNGRVRDRMGLSGTRSSGQWTNWPDGDHFDHIHLNGSMG
                     SGDIFNGGVGGSGVARWRSYVSKALKMNGLPATAAYINAWMSQIQTESGGNEKAIGGN
                     DGLAEGNATGLLQTKPGTFAANAFPGHGNIMNGFDNMLAAINYAKKRYGVAGMLQVIG
                     KGHGYANGGLITKDGLYRAGEGNKPEMVIPLTRKTRAIELMGQALAFLSGSNKKETKS
                     EINSVDNRHILQAIEKQSRMTNDLLSLLLSFFKSNNRSDKELALDIQKILIRRT"
     gene            18630..19502
                     /locus_tag="SCJ86_001673"
     CDS             18630..19502
                     /locus_tag="SCJ86_001673"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage baseplate protein"
                     /protein_id="HEG1169214.1"
                     /translation="MRTVILRNKLNEEIDLSTEDYFATEMSNLGFEVTKEHIGQWGSF
                     REIGESIELSEFQSSVMISVHGFREQQLYGSLVEFLANGPFELEFSFDSETMIRKCSL
                     KSLSKTEIDSQSSLLTDTLNLYFTSSWYSIKREKLIQNSTSLNTRGKVYSFVRPYVYT
                     KNVWEKKGVFKFSNNSVYLTNSTERMSPLKIRIIGECSNPYWEVIQNSKVVASDGYFI
                     NMKDTQILEVSSLLEDQTAILKDVGGVESSVYQQQDFTKSNFVQAPLGEFSVVFHVGH
                     ANVEIELYEERDLF"
     gene            19499..20548
                     /locus_tag="SCJ86_001674"
     CDS             19499..20548
                     /locus_tag="SCJ86_001674"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332431.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEG1169215.1"
                     /translation="MILAITLFHRDMNLYNERLISTEFEFGTDEIGEEVSNFITDKYV
                     HVKSGDFLLAKFIPSGKIAYFGVISSQEDKKINCKGLLSLADSEIPTVKISGANYEEH
                     IRRLIEYYLLNDPTKQLKNILEVKTESVTSHSYQAVDTNKRRLNAYIINGFKKYNVKW
                     YVKEIKGKKIYTGIRAVDDSIYIKDNSSEFNDWDVFVQSPGPGNENKLLIVDKAMKDI
                     ENPLVLSTWYLDDENNLTQDSTNKNIALPTVNLVNIYDQTQEDRPSYEEVAKSELKGN
                     AYSHEIKVNVVLNARNINVENIETGMLATIVYKEQTYKSVLTAWRISSSKKNIELTFG
                     NVRSRFMDYFEDNGG"
     gene            20552..21376
                     /locus_tag="SCJ86_001675"
     CDS             20552..21376
                     /locus_tag="SCJ86_001675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342061.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEG1169216.1"
                     /translation="MVSNVDGYQFENVKVSAENDARLYHVLYNRKNQVIDGYDQSMNL
                     SSSGLTVKVAAGAAIIQGRMVVVRQEESITVPANSSGYIALTVDLTQEVIPGSILPES
                     EEYEWTNNQVKLEFITKVIKGNLNNGDKVYNLPLCSVNSTGSTVSISKISDSYELTLS
                     KGEILWRGTALMHDTQTVQPSKKIWQTVSGFLLVWLPYENGQAIEDRYVTTPFYKERI
                     TFTNVLGEIVSGFDDYHKKWFSKRINYNSNTNIFTGAASNASGDNANMVLRYIVSF"
     gene            21394..>22031
                     /locus_tag="SCJ86_001676"
     CDS             21394..>22031
                     /locus_tag="SCJ86_001676"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290628.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BppU family phage baseplate upper protein"
                     /protein_id="HEG1169217.1"
                     /translation="MANLEIKLSANKRQPYLRHRVVGRVGDGGLTTINVQLLEEDEIT
                     PFVINLNGTLKFVGEVSNGNYTEGEPEIIDSTNGLISYTFTKSNFSTSHQFKQAYFEY
                     VDPNGKKVTFQNFIIDVLERVDINSEQAKYYISSLEKLQSEMQTTFNQFISDKQVQYD
                     QIYSKYNELVRLINESDKQVNDRIDKANNRIDQTNQQIGDLGKLKKMYSNSI"
BASE COUNT         7878 a         3297 c         4671 g         6185 t
ORIGIN      
        1 cttctaaatt gttcttatta tctatcatca gttgctgtct attgtcatta tgtcactgtg
       61 gtggaaaggt gtatcgctca tctaaaattt aggtgcaaac tgcaaggttc gattccttgc
      121 cagtgacttt ggtaaccgag gcatcggcgg tttaaaaata taggggtgcg caatttcgta
      181 cgcgttttgt gcatcttgta ggttgctatt acataattgg ttgggttaga ttgagttttg
      241 ggattcggta caaatgaatc gtcaaatgac tcaagcacag gatcggaaac gtccctgcct
      301 gtgcattaca tattagatca ctctttgagt ggtcttttta ttttaaaaaa gagaggattt
      361 tgaaaaatgg aattgattgt atttactaat aacggacaaa catatcattt ttatgaagtg
      421 gagaatttta agccaacaac aacaggattt agttttattt atactgggaa ggctacggga
      481 gttactcgtt cagctacgtt taacaataca tcaacagctg gttatgcgct tgcggaggta
      541 caaaatgaat catgagaagt ttatcgaaaa atgcaaggct attgtacgtg aaagaattga
      601 aaatgagatt gctgacctaa gtggagcagt gcctgagttt agtattttca tagtctggtc
      661 atgcaaaaca ctgcaaaata gcaaagcatt agtcagcgct agcttaaaag gagcaccgta
      721 ttttgaaatt acgttgaacg gggacaaagg tgaaatctat gtagatactt atctcaaaaa
      781 atcaaatgaa tgtatcaaag tctagcaggt gctaggcttt ttctttacat aaaggaggct
      841 gcataatgag aaactactgg tatgtatcac taactaatga atatcctcga accattggtg
      901 attgttcagt gcgtgttgtg cgttctgtac aaatcaaagg gaagtactct attgtcgaaa
      961 tgctaagaga agctacacca aacgaagtgg ataaatgcaa gctgatatat tgcggtcatg
     1021 gctattggaa agacgagtat atccaataca acattgagag gtacatcaaa tgaatgataa
     1081 ctatgattac atcaagttgt tagcaagtaa ttattagatg tttttaggag gaaaactatg
     1141 aaattaaaag attatatcag agaagggtat aacgttgtaa ctgcacccaa tctagcttac
     1201 aaaattcaag aagattatcc caatgcttta gtgtttactg atagagcttt agatgccatt
     1261 cctataggga agttattagt agatcgtttt tatagcaata atccagctgt tcttagtgct
     1321 aagccacttc aaaacgctta tgctattgaa cgctttactt gcgagtttac tggatatgag
     1381 acggtagtac caatgacagg tgagacagga agaaaagtaa ttcaacagat caaaaagagg
     1441 attgctgaat tgacgttaga tgatggaagt aatacgaacc ttctagaaat caaactaaga
     1501 gatactgact cagtaccaga agtttattat aaaggtcaac gaatggatga gatgccgata
     1561 ggtcttgtcg atattactta tcattgggat acggaagaat atgaatgcca agtaccaaaa
     1621 ggagctaacg acatcactat tgaatattat gattcacata ataacaagta cttagataga
     1681 aaaatcattg ggcacaagag agatatgtaa atgattgatg tttcaagtaa acaagcaaga
     1741 gctaggttct atggatcaac tgagtggagg aaacttagaa gtcaatgctt agagcgagat
     1801 cattatgagt gccaatggtg taaagcagag ggtagactaa caactcaaca tgattcaatt
     1861 cttgaagtgg atcatataaa ggagctggag tatcatccgg atttagctct agatataaac
     1921 aacctaagaa ccttatgcaa agattgccat aacaaaaggc acaaccgtat gaaatatcgt
     1981 ggccaacaaa agaaaagaaa gtgggatgat gaatggtggt aagaaaaaca gcaaacagag
     2041 atgatgtcaa acgagcagaa gcaatcgcta tggttgataa ggctattgat aagttggttc
     2101 tatttaagag tcagctcatt gaaggaacaa caactgtaga ggatatatct atcaacaata
     2161 gactaatagg taacgctaat gaagtaacag ggacatttaa tattaatgta gacattgatt
     2221 ataccttgag tccatttgca catattgtag aaagcaacga gcaagacggc tgataaatca
     2281 gtataaagcg aagatatata gagtgaaata cccccggtca aaagattttg agaagaaata
     2341 aaatttttgg gaaccggtgg atggggtcaa ctctgcaaat aaatgatgct attttcacac
     2401 gaccccctcc ccacgtagaa aggaagtgag ttgatggctg atctgcaaga gagaaatagg
     2461 cttgtttctg ctgaagaaag gcgtctaaat aaattgttta aggatattga taaggataaa
     2521 aagaaggtcg tttctggttt gattacgcaa gcggcacgct taaaaattct tcttgatgaa
     2581 atgtggattg atatttccga aaaaggtgat tatgagcttt tctctcagtc agaaaatcaa
     2641 aaaccttacg agcgggaacg accagttgct aaacagtata attcgagaga ccaagcatat
     2701 caacgtgtaa ttaagcaact tactgactat ttgccagaag aaaagcgcga acctacaaaa
     2761 aatgcagcgc tagatggtag tgatttgctg tgacaacatt aaagccattc tattttgatg
     2821 aatacgtaga tttatacgaa aaaggggtaa tcccttttaa taaagaaaga attcagttaa
     2881 ttacttattt aaaaaacgaa gtgctagttc gggatgatat ctattttgat gaagaaatga
     2941 ttcaaaagtt tatcaagtac actgaaaaaa atttttttcc tttagctaaa tatcaaaaat
     3001 ttatcacacc tttcattttc tgctatcaaa aagaggacga cgaggtgttt tttgatgaaa
     3061 ttctaaatag tattgcgcga ggcggcggga aaaatgggtt catgtcagca agagactcat
     3121 tttttattag ccctctctac ggtgtcagaa attatgatgt aacgattaca gcgaattctg
     3181 agaagcaggg aaaggtatct tttaaagaag tttatgaaat ggtacaaatg cgcaaactgg
     3241 aaaaacaatt ttatttgact aagatggcta tagtcaacaa tgtaactaat tcgattttta
     3301 gttataggac aaacaatcct aagactatgg atagtgcaag agatggctgc ttagaatttg
     3361 atgaaatcca tcagtttgaa tcttcagacc tagttgatat tcagcgtagt gggctaggga
     3421 aaataaaaca tcctcgaact ttttataatg gtacaaatgg tcatgttaga gaaggttttt
     3481 atgacaaaat gttggaacga gcacaaaaga tttttagtgg agagaacaag aatgatcgct
     3541 tgtttccctt tatttgtaaa ctggataaca ttgatgaaat ggataatcct aaaatgtggt
     3601 caaaggctaa tccaatgttc gaagaagatt cagcatattc aaaacgtctg ttttctacag
     3661 tgatgaaaga atataaaaaa ctagaagatg agccatcagg taggcgtgaa tttgttgtta
     3721 aacgaatgaa ttttacagaa ggtgatgcag aatcagatat tacgactcat gaaaaattaa
     3781 tggctactaa acaagaattg ccaaatttga atggtcgttc ttgtgtggct ggatttgatt
     3841 atgcaagtat tcgtgatttt gcaacagtgg gtttgctttt taaaattgat gataaattta
     3901 tctggcagca acatagtttt gctaggaaaa agtttctaga tacattcaaa ctgaaagcgc
     3961 ctattaagga ttgggagaaa aaaggtttag tgacaatcgt tgatgagcca tcaattgatc
     4021 ctcgtcattt aattgattgg ttaaatgaaa aaagaaaaat ccatcaaata gaaatcgttt
     4081 gtgccgacgg atttcgtatg gatttgctga aacctcttct agagaaagaa tcatataatt
     4141 ttgagtttat cagaaatata agaggtgtgc agtcaaaagt tgctcctatt attgaagatg
     4201 gttttgcaaa tgaacggttt atttttggtg atgatcctat gatgcgttgg tatacaaata
     4261 attcgtttat caaagaagac aaattaggga ataaaacatt tttgaaaaaa gagccagtaa
     4321 ggcgtaaaac agatgggttt catgcttttt tggcagcttt atataaacga gaagaaataa
     4381 ctgatatgga ttatgaggaa gcatttgata tgttggatga gattgaattt taaggtatac
     4441 tccttattga aaggggtagt tatagaagtg aaaaagagat attttctatt tgttacaata
     4501 tttataataa tagcaatttg tgttttgaca gtatttttac cacaaattac tgagtattta
     4561 aattatcgga tatttaccac aaaagtagat gatagcgtta ctagtttaag taggctagaa
     4621 tttataaaaa tatgtgcatc tgtgtttggt cctattttga ctgtaatggt atttgtgaat
     4681 actttaaata tgcagaaaaa aacagagcaa aagcaaaatg agcttgaagt aaagcaagct
     4741 aaggctgaga acgagagagc aaaaaaagag ctgttaacac agatagatcg tgagttctat
     4801 tttttattaa atatgtttat tgaaatacaa aaagatcaaa atgccaaaca agctgttatt
     4861 gctttacaga agttggcaat ttttaacgaa aagggtttta atcaagacta tgcatttact
     4921 tctaaagatt tatcagatag tgaaattggg agtacaattg aacaatcagg accgctgaag
     4981 ggcacagttg aatatataaa atttgaaaca tttgatgtaa gcaagtcgat ggggaattat
     5041 caaacaaaaa caaaagacgt tgctacaagt ttacacgatc agaaagtagc tatattgaat
     5101 gaacaatatg aaaagatgca aatatatctt ggacggtact tcaaaatgtt tcaccgaata
     5161 gttaaaacat taaatgagta ttatgacgat tataatgatt ttgatgtgaa gaggtataca
     5221 aaatatatag ggacattaag aacacaaatt tctccagctg aattccaagt gattttattc
     5281 aattctctat atataaaacg aggttttggc ttaggaattc aacttattgg aagtggtttt
     5341 tttggagatg attttgattt tgaaactaat caacattttg aaacaagtat caatgaacag
     5401 tggtttctat cactatcaac tgttgacagt gataactcta taaagcgaca aaaattatct
     5461 gaatatatta aagaattagg atccgtagaa tataaaaaaa tagctaattt tgaaagttta
     5521 tataaactct ttaatgaaac taagctctag aaatagggct tttttatttt tctttgaaag
     5581 gtggtgaaaa catgtgagct tatttgatat ctttaagcag tcaataagaa atgaagagcc
     5641 ttctgagtgg ataccggatt ttgtatatgg agaggaagta tctgctcggg catacctcaa
     5701 aattatggca aaaaatactg ttttagattt tgttgcaaga actatgtcaa cgttagaagt
     5761 taagtttaaa aataaagatg ggacatcaga ctgggattat attctaaatg ttcgacctaa
     5821 ttctgatatg tcagcagcta cattttggca aaaatttttt tatcggctct tagatgacaa
     5881 cgaagtgtta gtaatattta cagatgataa tcaattgctg attgctgatg attttagtcg
     5941 aacggagtat gccgtgtatg atgatgtttt tacaaatgtg atggtaaaaa attttgtctt
     6001 tgaacgttca tggaacatgt ctgacgtcat ttatatggaa tataataacg aagaattaga
     6061 aaggtttaca cgaggtttat ttgaagatta cgccgcgttg ttcggacgga ttctagaagt
     6121 tgcaatgcgt aacaaccaaa ttcgtgggtc tgtatccatt gattcaacgg gaacggcgaa
     6181 tgatgaaaaa ggaaaagatg gtaagactag atctcaaaga ttacaagaat atattgatag
     6241 gatttggaac tcttttaaaa ctaactcagt tgcaattgtt cccaaagtga aaggctttga
     6301 gtatgaggaa tacaccaata aacaaggcgt atcgaatcaa tcgctagatg aactgaataa
     6361 gatgaagtcg tctttaattg atgatgttgc caacgccata ggagtaccta cggcgcttat
     6421 ttatggtgaa aaagctgaac tggattcaaa tattaaagct tttcgtaaat tgtgtattgc
     6481 tccactagtt aaaaaacttc aagacgaatt ggtggcaaaa ataattagtc gtcaagagta
     6541 caaaaacggg gaacgtatag aagttaaaaa agttctccaa aaagatctat tagagtatgc
     6601 tactcaaatt gataaaataa catctggtgg tgcgttttat attgacgaag ttcgtgatga
     6661 agttgattat gatgagttgc caaataatga aggaaaaatt cgtattcgta cgaaaaacta
     6721 tgaagaaact gtgaagggag gtgagaacga caatgccgaa agctaaaaaa gtaccatttc
     6781 agtttactaa cgaaattcag aatggtaagc acattctcac cttgagtgga aatgtacaga
     6841 aaaaatattg gcgtgatgat gatgtcataa acgctaaaga tattcgtgac tctctagata
     6901 ctgtaaccga tgatattgta atcaagttga acagcccagg cggtgatgtg tttgaaggga
     6961 ttgagattta caattattta aaagatcacc catcaaatat tactgtagaa gttacagggc
     7021 ttgccgcaag cgcagcaacg tttattgtcg ctggtgccaa tgatgtgatt atgaatgttg
     7081 ggacttcttt gatgattcat gaagcttcta catttgcttg ggggaataaa caggacatcc
     7141 aaaaaacgtt aaacgcattg gaaacgatcg atgattctat cttagctatt tattcagata
     7201 agactggcca atctgttgat aagttgcgtg aatggatgaa tgaagaaaaa tggtttacag
     7261 cagatgaagc tgtcaaatat ggatttgcca attctgtgaa gcgtgaagaa ccacaaaatg
     7321 aacctcaaaa tattgcagcg atgattcaag atgctgttgc cgttgcaata gcgaatttaa
     7381 aggaacctgt tactaataaa atcgaacaag aatcaaaaca aaaatcatta atcgcacgat
     7441 tgcgaaaagg agaataaaga tatgttaaaa attacagata aaactgcaga tgcaaaaaag
     7501 gcatttaatg ctatttcttc gaaagaagat gcaacacccg aacaaattaa tgctgctcta
     7561 gaagaatatg taaccgccat tgcagaagat gccggcaaac aggtgcgtgc tgaatatgaa
     7621 gaattgaaaa atgtgacgga caaccgtgta ttagaggctc gtggaattcc tacactaaca
     7681 gcagaagaaa caaagtttta taatgaagcc gtaaaatctg gaggtttcga ttctgacgta
     7741 gtttggccag aaacgatttt ggaacgtgtt tttgaaaatc ttcaaaacga tcatcctatt
     7801 ttaggcctca ttaactttac accaactgta ggtcgtgtca aagtaattag agcacgtcgt
     7861 aaaggtgttg ctgtttttgg accattgcat aaagaccttg aaggccaact agatgctgaa
     7921 tttggtgcaa ctgaatttgt tcaacttgcg ttgaccgcat ttttcttaat ttctaatgat
     7981 accttagatt taggaccacg atggattgac cgttttatca atctttcttt aagtgaagca
     8041 gtacgtgatg tttgggctaa aaaaattatt acaggttctg gtaaagatga gccaattgga
     8101 ttgcttaaag atctagatgg tgcggtcaca agtggtgtat atccagataa agctagcgca
     8161 gggacattaa cttttgcgaa agagaagatt gttcttgaac ttgcaggggt gatgaaaaaa
     8221 ttatctaaat acacatacaa agtagatggt gacgatgaag gtgaagtgaa ataccgttct
     8281 gttgctggac gagtgtatct aattgttaat cctgttgatt actgggatat cgtagctaga
     8341 gttacatttg caaatttaaa caatgtgtat gggactacga tgccatttat cgctgttgat
     8401 catatcatcg aatctgtaga cgtaccagaa agtaaatgta ttgcatacgt tgataaagag
     8461 tatgaagcta ctcaatcaag agctgaaaaa gtctatgtat ataaagaaac ttttgcaatg
     8521 aaacgcgcaa ctttgtatgc tgtcgacatg cttggtaatg gctatccaac aaataatgat
     8581 gcagcacaag tatatgactt ggactttaca gcgggggagt aatgtcccct gcaattggtg
     8641 agataacacc gacgactgat ggggcagtca ttaatctttc gtaaatgggg ttagagtatg
     8701 aacactgaag aatttataaa agaatataag gcccgatttc gtattttcca tacttccgaa
     8761 gatgaggata tcgggaaaca gttagagagc gggtttgccg atataaaatc tctaatcggc
     8821 agttttgatc cttccactta tgagaaagga aaagaattag tttatgaacg tactcggtat
     8881 ctacggaacg aatcactaga gtacttttac gacaattttc aaacaatgat tatggatgca
     8941 tcaattgatc tggcaggtgc tggatatgcc aattaaatca aattataaga agcctaaaat
     9001 tgtagctggt gatctaaata cgccagttac tttttttgag tttcaaccta ataagggtcc
     9061 agaacctggt gatgaagaaa aaaaggaact gtatttttgt acagcactag tttacaaccc
     9121 atcaatgaaa gatcgtgaca ttcttggcac aaaagaaggc gttacgataa aaatacggga
     9181 tccccatcaa tcttatattc ctactaacaa acataaagta aggatattag attatcgata
     9241 tggagataaa gtttgggaaa taatcgatgt ctcaccggat ttggaagaaa attcaattgt
     9301 aaagatagta ttagaattaa ttacttaaat ttggtgataa atgatgagta acgtaaagtt
     9361 tgaaggcgtt caagaaacaa tccaagcttt agaaaaaaag tatggagaaa agaaaactaa
     9421 gacattaaca aaaaaagcaa tcaatgttgg cgctgaaaaa gtagaaaaac agttgcaaac
     9481 ggacatgacc gtttttaaag acaaaggtta tacgatcgct gaggttgttc gcaagaacgc
     9541 tacgtataga gattacaagg cagaagctga aatcggttgg aatggtccac atcaacgata
     9601 tagaatcatt catttgaatg aatggggata tacacgaaag ggcaaacaaa ttagaccgag
     9661 aggatttggt gtaataacaa aatctcttaa gaattccgag ctgttgtact ttgatgctgt
     9721 aggagaggag gttaaaaaga gtttatgaaa gatatgctta atatcattta cgaggctctt
     9781 tgtttgaatg agtatattca ttcaatgacc tacaacgaga ataccgagca atatcgtatt
     9841 aaattttatg agcaacctga aaccgccgat aaaacaggtc caatgatcac aattcgtcct
     9901 gtagatacac ctaacgaagc gtaccacgga agtaacaaag agctttctgt ggagcatatg
     9961 atacagattg acgttgaatc gaaatataga gcaacatgca agcagattca atatgagata
    10021 aaaaaagaga tgaaaaaact aggctttggc caaatttccg gtcaagggtt agatgaatac
    10081 tttactgaaa caaaccgctt tgtagacgcg cgtcgttacg aaggaaatac aaaaatttac
    10141 gataccgaat attagaaatt aagacacgaa aataacgtgt ctttttttat tacccaaaat
    10201 taggaggaaa attttatgac tttagtagga ttcaaaaaaa tgacaatcgg ggtctttgac
    10261 agcacaggga agattccagc agcaaattta tatgtaattg aaggtgaaca aaacaaaggg
    10321 gcgactgtat ctgctgaaat cagtgggcta tcaaaagaac catcaaaagt ttacggatcg
    10381 aacatcgctt actatgtctc tcaaaaagga acaggcgatg tttcagctac ttttggattg
    10441 ttagatttac caacagaagt aaatgacaaa attcttggtt acaaaaccga tacaaacaaa
    10501 atcagcttct tgggagaaga tactgagcca ccatattgtg caattttgat ggaatcggag
    10561 gatttgaacg gcgatacagc aatgcttgcg atgttcaaag gaaaattcag tcgtgaatct
    10621 attaatctta acactacgac aaatgaagct ttcgaaccag aagcagaaga atatgtattt
    10681 tcagcgattg ccaatgatgt tgaaggtgat gctaaaggcc aaacagtcgc aaaatatgtt
    10741 ggagacgaag aagcttctat tactggtttg aaagagatgg tatttcctgc agcgggggag
    10801 taactagccc cgttgttgga gcaatcactc ccactattaa tggggcaaca gttgaattaa
    10861 gttaggagga taactatgcc agatacattt aaaatctata aaaaaaatgg tactgaattt
    10921 acaaaagtcg cagagggtga aagtccactt tctattactg gaattgcagc aaatactcaa
    10981 gttgctaaag gagattatca agctacgcgt gtggtcggag gtcaagaatc agtaaaagta
    11041 gatattcctg cattcaccac tcttccaatt tcagttactg gtgtgacgtt ggataaaacg
    11101 acagctgaag ttgaacaagg cggaacctta aaattaactc ctactgtaac accagcaaat
    11161 gcaacagaca aaacaggaag ctggagcagt tcgaatacag ctgtagctac ggttactgga
    11221 ggcaacgtta cagtaaaaac agacgctgaa gttggcggaa ctacagaagt ttcatttact
    11281 actacggatg gcggtaaagt agcaaaatgt actattacag ttattgaaaa agcagcgggc
    11341 tagtcttcgg actagctttt ttatttggaa aataatggag gaatctaaat ggcaaaagta
    11401 agaattgaat tgaaagaaga atcaggaaag aaagtctatg aaaacataga cacaacaggg
    11461 aaagattatc gaaaagcttt agaaacaatc aaaaagctaa atgaagacgg agtaatgatt
    11521 tggaatcgtt tagacattta tttggatttt gctgtaggta tcttcaggga tagcaaattg
    11581 acagctgaac aaattcttga tggcttacca tctgagaagg taatgagcac attggacgac
    11641 atcctaggag aagtaatggg gattgaaagt aatccagacc cagacgcaaa aaagtaacac
    11701 ccgaagaagc agaagaaatg tacttaaacc tatgcagaga gttagtgaaa gcgggatggt
    11761 cactatctga catagaagat aattcctttg aaatgatgat gaaaattgcg tgcacttcgc
    11821 ctaagaaaga gaaacaaaaa gaagttgatc ttaaagactt cttgaaatcc atgtagagag
    11881 gaggtaaaca tatggcaaat ggaaaacctt taggcaatat gattatcaaa ctagacttag
    11941 acagttcagc tttttcaaaa ggtctagccg gagctaaaaa cgctgttaac catcaaatga
    12001 aagcaatgaa atcacagatg caagtaatga actcttcagg aaattctctc ggtgctttac
    12061 aagcaaaata caatggttta ggcggagttc tacaagcaaa tgaaaagcaa gtagaattgc
    12121 tcactaaggc ttataaggat agttttgact ctaacggaaa cgctactgct tcgactgcta
    12181 aatacgctaa tcaattgaat caagcaacag ctagatcagc aagttacgaa tctcagatga
    12241 aaacaactgt tggacagatt gctcggatga aagtggaaac ggaagggttt acggggaaac
    12301 taaaggctaa ttctgatgta ttgattaatt ctgggaaaaa aattgaagcc tttggtaaag
    12361 gagtttctgg gataggatct gccctaactg ttggagtaac agctccaata gtggcaggcg
    12421 caactgctgt tactagtgca gctatttcat gggaaagtgc ttttgcaggt gtaaaaaaga
    12481 caaatgatga agttgtagac tcgaatggaa acgtagttta ttcctatagc gacttagaga
    12541 aaggtctgcg taatcttgcc acccaattac ctggtagcca tcaagaaata gcaaatgtag
    12601 ccgaagcagc cgggcaatta ggtataaaga ctgaaaacgt agttagcttc actaaaacga
    12661 tgatagacat gggtgaatcg actaatatgt cggctgaaac tgcggcaact tctttagcac
    12721 gatttgctaa tattacccaa atgtctcaaa aagactttga taaattagga tcagtcattg
    12781 ttgatctagg taacaatttt gctacaaccg aatcagaaat cactgaaatg ggattgcggt
    12841 tagcgggagc tggtaaacag attggaatga gccaaggcga aattctagga tttgctactg
    12901 ctctatcttc tgttggtgtc gaagctgaag cgggtgggtc tgccttttcg aaggtcatga
    12961 tccagatgca actagctgta gaaaaaggat ttggcgcttt tgatcagctg aaacagatgg
    13021 cagaagaaca aggtgttccg tgggttaatt tagtaaatgc tgtgagagac ggtggaaaat
    13081 cgttaaaagc agtatcagag caaatgggat tcacttcatc tgatttaaag aaaatgtata
    13141 aagaagcgga caattcgaaa agttcgcttg aaaattttgc gaatgtggca ggtgttacaa
    13201 gtgaacaatt ccagaagttg ttcaagagtg atccttcaga agcaatcatc aagttcattc
    13261 aagggctgaa agattcagaa aaacatggta cttcagcaat caaagttctg gatgacatgg
    13321 atataaaaga agttcgacta cgcgacagtt tattgagagc tgcaaatgct agtgacatat
    13381 tcaaaggagc aattgaaaaa ggaaacggcg cgtggaaaca aaatacagct ttaacaaatg
    13441 aagctaataa aagatacgaa acaactgaat caaagctaaa gatattgaaa aacgaagtga
    13501 ttgatacagc aatcgatctt ggaggtccat ttgtagacgc gttgcgtgat ggtctaaatg
    13561 tatcgaaacc attgataaaa aacttaggga atatggcgaa agctttctca gctatgagtg
    13621 atgatcaaca aagaaacatt atcaaatggg ttggattagc agcagcagct ggtccagcgc
    13681 tgaagctttt aggaactggt acttcagtaa taggtaaagt tacttcaact actggaagat
    13741 tgacgaaaag ccttgttgat ttagcagcga aagcagcaga aaagaaggct atggatgcct
    13801 ttgcatcgtc ggtaacaaca gctggtgctg cagctgcaaa gacagcagga gctggaggta
    13861 ttggcgggtt agtccctatg atgggaaaaa cagctagtgc tgcaagtact gctgcgggcg
    13921 ccagcggaat aggcgcaatg acaacgtcac taggtttgtt aggtcctgct ctacttggta
    13981 ttgttggcgt tggtggcgca ctggcagttg gctatggagc atggaaatta tttggtgaag
    14041 aagcttggaa ttcctcgcaa agagtaaagc aatggggaac agatgtcggt gaggagatag
    14101 atagtactct tgacggagta cagaagaata ttgaaggtgc aaatggtcaa ttcggtcttc
    14161 tcaaagaggg ttttactgtt gatgatgcaa ataatatggc ggctaatttc gcaaccgctg
    14221 gaacatcttt ggaaacatct ttaacgggta gaataaaggc attagacgat gcactaaaag
    14281 ggctacctca atcagttcag gatgccatga aagaagtagc tgaatcagaa gaagaaacct
    14341 tagggcaatc tctttctaaa ataaaagaca acaatgaaga aataaaaaaa atcagagaaa
    14401 gagccgcaaa tcagaacaga gacttgactg tttcagaact acaacagata caggatctaa
    14461 tgaaaggaac ttctcaagcg tatgtggata cgctcgatgt ttctgctaaa gacagaaaaa
    14521 agatactatc agccatgaat ggtgatgttg cacaagctac agaagaagaa gcgaagacgt
    14581 ggttacaatc tttagcgaaa caaaaagctg caactaccga aaattataga caaatgcttg
    14641 aagaaaaaaa ggcggcttta aaggaaaagg gagtaagcaa agaattcatt gatgcattag
    14701 aagatgaaat gaatgattac atcaaaacta caaatcaagg attcgatagc caaatagctg
    14761 ctataacaaa taaatatcca caattgatta acgaagtgag tttatcaaat ggtcaattat
    14821 ttagcaaaac ggcagctgct atggacaata ctggtcagta tacaaaagtt tttctcgaaa
    14881 gtaatgaaaa gattttagcc caagcgaaga aaacatcgga tgaaatagca gataatgccg
    14941 aaaaaaatgc agaacaactt ggtatggtag ctgatgaggc tacgcatgct ggatcaactt
    15001 ggaatggctt agttttagac gaaaaaacag gaaaagtaaa aactaatgta caagaagtag
    15061 tgaatgaagc aacgaaggat ttcactaaat ggaatgaatt gaaacctgtt gttcatgatg
    15121 cgaaactaac aacgaatgca aaagatgtca tttccatagc ggcagtacaa aatggctatt
    15181 gggatgatat ggaatggaaa gaaaaagaag cattattaga agatgagtgt tctaaaaatg
    15241 tatacaaggc tttagaaaat gctggtaaat ggaatgagtt agatattcct tctaaagaag
    15301 caatcctaac ttcaaatact cctgaagtta tgggtgaaac tctcatgaat ttgggattat
    15361 gggatactta caaacttcaa gtgaaagatt tagatttgaa tgcgtataaa gtatacgatg
    15421 ctatcgatgg atcagaagaa aaattaaata gttgggctca attagataac cccacaaaag
    15481 aattgttatt agataactca gatttttcta caaaactttt taattccgaa gaaatgttta
    15541 aacgttggaa tcagttatca tctaatgcaa agtttctttc ggctaataat actaaattta
    15601 cagaggttct tttatcatct gacagaatgt ggaatcaatg ggtaaacctt cctgataatg
    15661 agaaaagatt acttgcagat aacgaagatt tagctaccac tgttttatct tcagaagaaa
    15721 tatacggaag atggactcaa ttaccagata atcaaaaata tatgttagct gacaatactg
    15781 acttagcaaa caaaatattc gcttcagaag aatcatggaa tgcatggaaa gcaattccag
    15841 acacagttaa aaacttgctt ggtaataata ttgatttact ggctaaagtg aaagacggga
    15901 ctgtaagtgt agaagaatat aacaaagtta tgccacttct taaaactcta tatggagata
    15961 acgttaattt aaaagaggca atcagtgggg ctaaaagctc tatagagaat tacaacaaaa
    16021 atatttttcc tagtgataaa actgcagtag gacacgatga ggcttcacaa gctgctaaag
    16081 atgcatttga agcgttcaac atcttccaaa caaaaatacc agataaaatt actaaaactg
    16141 tatcagctga ctttataggt cctatgccga aaaatgcgaa aggaacaaac ttccatcctg
    16201 gcggagcagc tatggtaaac gatcaaaaag ggcctactta tgaagagctt ataaccttgc
    16261 caagcggaga aacttttata cctaaaggac gtaatgtcgt tttagactta ccaagaggtt
    16321 cgaaagtact gaacgccact aaaactaaac gtctagtgcc taaatatgct gatggtatag
    16381 gaaacataac gacggtttca tctacgctaa acttagatgc tttaatttta gctattaacg
    16441 agctaacgac ggtattgaga actcaactga tggcaggaac gactgatgct aaagatacta
    16501 aactatcaga tgaaacaaaa gttgccgatc cgattattcc ggacagtctt tctgaaaaat
    16561 cagatcagta tctaggaatt ggagctcaat ggttaactaa tctaatgaat ggttggaact
    16621 cagcggttcc tcaatatatg aatagtgaaa tggtttttat tacaaactat ctcaatgcgc
    16681 taaaacttca aaacaatcct aattatacgc aaggagcaac atggaataag aacttactga
    16741 atggatggaa tagtttaact gggaccttta ttgctacaat caattcattt tgtaatcaag
    16801 caatggtcac gcttagaaac tacaacactc ctatgtacaa caatggccga acgtggcagc
    16861 aaaataatct taatggttgg aattcattgt atggatcatt catagcccgt gtaaatcagc
    16921 ttggtaacga ttcgattaac aaccttcgtt ctaaaagtgg tggtttttac aatgcaggga
    16981 cattcttgtt gcaatcttta ataaacggaa tgaattcgtt aggtaattct ctttctacta
    17041 caatgaacaa tgtcgctaat acaatggttg gtggtatagg caaaggagtt aatggcgtca
    17101 tatcgggagt taactatgtg cttaaggaag tcgaatcaag taagaatatc ggaaactggc
    17161 caattccaca atacgctaag ggaactgatg gacatcctgg tggacttgct atgattaatg
    17221 atcaaaaagg cctagttcac gaagaatatg ttcagatgcc agatggacgt agttttattg
    17281 ccaaaggtag agatcttctt gttaatttgc ctaaaggtgc tcaggtctta aatgcttctt
    17341 taacaaagaa attaaaggaa cgtttaaata ttccacacta cgaaaaaggt attggcaatt
    17401 tagatattgt tgatttgtta gatgatgaaa aaagaatgtt agaattctta actagcaaag
    17461 ttgatttttc aggcattaat gaaccatggc ttgatatgac taaatcagga acgtcattaa
    17521 tgtccaaagc tgcaaataca atgcttcaat caaaattaag tgaattcttc actcatggca
    17581 attttgatgg tgcagtaaat gccaatggtg tttatcaata tttagttgat gtggcacaga
    17641 aagtaatggg taagttcccc ggacttacag taacttctgg atatcgagca ggagatgcct
    17701 actatcacgg aaaacggcaa gctatcgact tggcttatcc aggtatttct ggagatccga
    17761 gatatacagc agcagcaaat tacgcttttg aaaaattccc ttcaaaaatt gcgtatgtca
    17821 ttacgaatgg acgtgtacgt gaccgcatgg gattatctgg aacaaggtca agcggacaat
    17881 ggacgaattg gccagatggc gatcacttcg atcatattca tttaaacggt tcgatggggt
    17941 ctggcgatat attcaatgga ggagttggag gaagtggtgt tgcacgctgg cgttcttatg
    18001 tgtctaaagc attgaaaatg aatggattac cagctacagc agcgtatata aatgcctgga
    18061 tgtcccaaat ccaaacggaa tctggtggta atgagaaagc aatcggcggc aatgatgggc
    18121 tcgccgaagg caatgcaaca ggattacttc aaacgaaacc cggaactttt gcagctaacg
    18181 cttttcctgg tcacggaaat attatgaacg gatttgataa catgttagca gcaatcaatt
    18241 atgctaagaa acgttacgga gttgcaggta tgttgcaagt tatcgggaaa ggacatggat
    18301 atgccaatgg cggcttgatt accaaagacg gactatatcg agcaggagaa ggaaataaac
    18361 cagaaatggt tattccattg acaagaaaaa caagagctat cgaacttatg ggtcaagcat
    18421 tagccttcct ttctggaagc aataaaaaag aaacgaaaag tgagattaat tctgtagata
    18481 atagacatat cctacaagcg atagaaaaac agtctcgtat gacaaatgat ctgctatcat
    18541 tattactctc gttttttaaa agtaacaatc gatcagataa agaactagct ttagatattc
    18601 agaaaatttt aatcaggagg acatagtatt tgaggacagt aatattaaga aataaattga
    18661 atgaagaaat tgatttgtcc acagaagatt attttgctac tgaaatgtcg aatctaggtt
    18721 ttgaagttac aaaagagcat attggacaat gggggagttt cagagaaata ggagagagta
    18781 ttgaattgtc tgaatttcaa tcatcagtta tgatttctgt tcacggattt agagaacaac
    18841 aattatatgg ttcattagtt gaatttctgg ctaatggacc ttttgaacta gaattttctt
    18901 ttgattcaga aacaatgatc aggaaatgta gtttgaaatc attatctaaa acagaaattg
    18961 attcccaaag ctcattgcta acagatactt tgaatctata ttttacttca agctggtatt
    19021 ctattaagcg tgaaaaatta atacagaatt caacttctct caatacacgt ggaaaagttt
    19081 actcatttgt tcgaccatat gtctacacca aaaatgtatg ggaaaagaaa ggtgtattta
    19141 agttcagtaa taattctgta tatttgacaa atagtacaga gcgaatgtct ccgttaaaaa
    19201 taaggattat tggagagtgc tcaaatccat attgggaagt tatccaaaac tctaaagtag
    19261 tagcttcaga tggctatttt attaatatga aagacacaca aatattagaa gtgtctagtt
    19321 tgctagaaga ccaaacggca attttaaaag atgtaggtgg agttgaatct tctgtttatc
    19381 aacaacaaga ttttactaaa tcaaacttcg tccaagcgcc tctaggagaa ttcagcgttg
    19441 tttttcatgt tggacatgca aatgtagaga ttgaacttta tgaggagcgt gatctatttt
    19501 gattttagcc attaccctct ttcaccgaga tatgaattta tataacgaac gtttaatttc
    19561 gactgagttc gaatttggaa cggatgaaat tggagaagaa gtaagtaatt ttattacaga
    19621 taaatatgtc catgtaaaat caggagattt tctactagca aaatttattc ctagcgggaa
    19681 gattgcttat tttggagtga ttagctcgca agaagacaaa aaaataaatt gcaaaggatt
    19741 actcagtttg gccgatagcg agataccgac tgtaaaaata tcaggtgcta attatgaaga
    19801 gcatattcga cgactgattg aatactactt attgaacgat ccaacaaaac aactaaaaaa
    19861 tattttggag gtaaaaacag aaagcgttac ttctcattct tatcaagctg ttgacacaaa
    19921 taagagacga ttgaatgcat atataataaa tgggttcaaa aaatataatg ttaaatggta
    19981 tgtcaaagag attaaaggta aaaaaatata tactggtatt cgagcagttg atgattcaat
    20041 ttatattaaa gataattcat ctgagtttaa tgactgggat gtttttgtgc aatctcctgg
    20101 accagggaac gagaacaaat tattgatcgt agataaagca atgaaagata tagaaaatcc
    20161 cttggtattg tctacgtggt atttagatga cgaaaataat cttacgcaag attcaacgaa
    20221 taagaacatt gcattgccta ccgttaattt agtaaatata tatgatcaaa ctcaagaaga
    20281 tagaccttct tatgaagagg tagctaaatc ggagcttaaa ggcaacgcct attcacatga
    20341 aattaaagtt aatgttgtgc taaatgctag aaatatcaat gtagaaaata tcgaaacagg
    20401 aatgttagcg actattgttt acaaagagca gacttacaaa tctgttctaa ctgcctggcg
    20461 aatatccagc agtaaaaaga atatagaatt gacatttggt aatgtccgaa gtcgttttat
    20521 ggattatttt gaagataatg ggggataaaa aatggttagc aatgtggatg gatatcaatt
    20581 tgaaaacgtg aaagtaagcg cagaaaatga tgctagactt tatcacgttt tatataatcg
    20641 gaaaaatcag gttattgatg gttacgatca gtctatgaat ttatcttcaa gcggattaac
    20701 agtaaaagtt gctgcaggag cagcgattat tcaaggtcgt atggtcgttg ttcgacaaga
    20761 agaaagtata acagttccag caaactcaag tggttatata gcattaacag tggatttgac
    20821 acaagaagtt atacctggat ctattcttcc agaatcggaa gaatatgaat ggactaataa
    20881 tcaagtcaag ctagaattta taacaaaagt tataaaaggt aatttaaaca atggtgataa
    20941 ggtctataat ttaccactat gctcagttaa ttctactgga tcaactgttt caatctcaaa
    21001 gatatcggat agttacgagc tgactctctc taaaggagaa attttgtgga gggggactgc
    21061 gttaatgcat gatactcaaa ctgtccaacc ttcaaaaaaa atttggcaga cagtttcagg
    21121 ttttttgtta gtatggctcc catacgaaaa cgggcaagca attgaggata gatatgtaac
    21181 tacgcctttt tataaggagc gtataacttt tactaatgtt ttaggagaaa ttgtttcagg
    21241 atttgatgac tatcacaaaa agtggtttag taaacgaata aactataatt cgaatactaa
    21301 tatatttaca ggtgcagcaa gcaatgcaag tggagataac gcaaatatgg tgcttaggta
    21361 tatagtttct ttttagttag gaggtatgga agagtggcaa atttagaaat taaattatct
    21421 gcaaataaga gacagcctta tctacgtcac cgtgttgttg gtagagttgg tgatgggggg
    21481 ctcacaacaa tcaatgtaca acttcttgag gaagatgaaa ttacaccttt tgtaattaat
    21541 ctaaacggta ctttgaaatt tgtgggcgaa gtttcaaacg gtaactatac cgagggagaa
    21601 ccagaaataa tcgattcgac taatgggtta attagttaca cgttcactaa gtcaaatttc
    21661 agcacgagtc atcaattcaa acaagcatat tttgaatatg tagatcctaa cggcaaaaaa
    21721 gtaacttttc agaacttcat catagacgtg ttagaacgag tagatattaa ttcggagcaa
    21781 gcgaaatact atatttcttc attggaaaaa ttacagagtg aaatgcaaac cactttcaat
    21841 cagttcatta gtgataaaca ggtccaatac gatcaaatct actcgaaata taacgaatta
    21901 gtaagattga taaatgaatc agataagcaa gtaaatgatc gtattgataa ggcaaataat
    21961 cgtattgatc aaaccaatca gcaaatcggc gatctcggca agctgaaaaa gatgtacagt
    22021 aacagcattg a
//
LOCUS       DARBET010000039        22030 bp    DNA     linear   BCT 07-NOV-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4027
            SAMEA114569841-rid20112183.denovo.039, whole genome shotgun
            sequence.
ACCESSION   DARBET010000039 DARBET010000000
VERSION     DARBET010000039.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA114569841
            Sequence Read Archive: ERR12203666
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 22030)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 22030)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001972618.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001972618.1
            Genome Coverage       :: 132x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/07/2023 13:18:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,041
            CDSs (total)                      :: 2,975
            Genes (coding)                    :: 2,908
            CDSs (with protein)               :: 2,908
            Genes (RNA)                       :: 66
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 67
            CDSs (without protein)            :: 67
            Pseudo Genes (ambiguous residues) :: 0 of 67
            Pseudo Genes (frameshifted)       :: 26 of 67
            Pseudo Genes (incomplete)         :: 28 of 67
            Pseudo Genes (internal stop)      :: 20 of 67
            Pseudo Genes (multiple problems)  :: 6 of 67
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..22030
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA114569841-rid20112183.denovo.039"
                     /isolate="VRE4027"
                     /db_xref="taxon:1352"
                     /country="Denmark"
     gene            complement(61..1371)
                     /locus_tag="SCJ86_001677"
     CDS             complement(61..1371)
                     /locus_tag="SCJ86_001677"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 28 protein"
                     /protein_id="HEG1169218.1"
                     /translation="MRTKKGIINALNFKTNDTQQDDTIILQKAIDQGAKEHLPVFIPK
                     GIYLVGALFLKDKSHLIFEEGAVLKGRTDIEAFPEIDTRVAGVEMKWPAAILNVLSAK
                     DILIEGKGIIDGQGDHWWELYWGKDQKSGTRAEYDQKGLRWIADYAIKRPRACLLYHA
                     EHVVVRDLIFQKSGFWNLQITYSNDVLVEKVIIRNNDGPSTDGIDIDSSTNVRVYECD
                     LACGDDCIAIKSGRDGNGARVNRKSSRIEVARCKIRSGYGVTIGSEVSAGVSDVYIHD
                     INFFQSDCGIRMKSSKERGGVIENIRVENLNMIDVQFPFSWIMDWHNEYNRKNSNDLA
                     QMPESWQAVAEEIPESKQMTKVKDIHIKNVQACLSPGYSLPSRAFDLVAFPEKPIEDV
                     CFSHCQITAKEFGRIEAVQNLCFKSCDLSIETGNTAANNTFDNR"
     gene            complement(1383..2801)
                     /locus_tag="SCJ86_001678"
     CDS             complement(1383..2801)
                     /locus_tag="SCJ86_001678"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEG1169219.1"
                     /translation="MLSQNAVTANTVKKEKKEFGISDKIGYMFGDLGNCFILGLVNSF
                     LMIYYTNVLGVSGAIVGTLFLISRILDAFADVMVGRLCDVSELTETGRFIPWIRKMKY
                     PFCLVTVILFLPFVQHLPMTVKIAYIFVSYIVYGIFLSTINIPYGAMAAAISSNPNDR
                     TSLSTFRSIGSAIGGSTTGFFIPILMYTHNKSGQQVVSGNHFFWISIGCAAIAFVAYM
                     LTCKLTTERVRVVKNEKVSASQLVKGLLANRALIILVIVDIFIVINQILAGTNMTYLF
                     NDYFHNKQAMSVALLFTYGTVVVLAPFATKLTKLFGKKEASVFALLASSCMYLLMYFL
                     HITNAWVYLILLFIATLGSGLFNLMVWAFITDVIDYHQYKTGLREDGTVYGVNSFARK
                     VGQACAGAVGGFMLALIGYQSSTTGGAIQSVLVQERIYTMANLLPAFCLFVSAIILLV
                     GYPLNRSKTVFIGEELKKINER"
     gene            complement(3041..4609)
                     /locus_tag="SCJ86_001679"
     CDS             complement(3041..4609)
                     /locus_tag="SCJ86_001679"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338742.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 43 protein"
                     /protein_id="HEG1169220.1"
                     /translation="MQTIKVKNPILRGFNPDPNILRVKERYYIIVSSFEWLPGIRVYE
                     SKNLVNWSHTTDILTTQVSLQGNPTNGSIWAPQISYADGLFYLVYTDVKSAGRPFKDV
                     HNYLITASEITGPWSEPIYLNSNGFDPSLFHDLETGKKWLVNELWDYRLDTPNKSSGI
                     VLQEYDAEKKKLDGPIYKIFAGTELAKTEAPHLYFRNGYYYLMTAEGGTGRGHSVTVC
                     RAKNITGPYELDPGFPMLTASDKPASTLQCTGHGSLVQTPSGRWYMTYLCTRPLNGAA
                     ILGRETAIQEVYWTSDDWLRLKDSQTTPMEEIMIETNEEVQQEKDHQFMDTFEGELKK
                     NWNARRILPNADWCDTKCRPGFLRLISGESLQSTFEHHLLAIRQTDFTFDAETAFEYM
                     PNNFNQMAGLVLYLNESNYIYCYVTWNEDKGKCLRMIESENGVAQIEDWTIPLTEKKT
                     FLKVTVKKETAQFHLKDVSTEEWKKVGPQKNMCILSGGFTGNFIGIAVHDLNKKRGSY
                     ADFEFFKYDGKDHL"
     gene            complement(4613..4894)
                     /locus_tag="SCJ86_001680"
     CDS             complement(4613..4894)
                     /locus_tag="SCJ86_001680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319614.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4064 domain-containing protein"
                     /protein_id="HEG1169221.1"
                     /translation="MKRKWELLLGMVGGSLSLIFFGGLAVTLSNMSASEFKKSYQSLA
                     VDHSTLSLENTFGLLQDMTGLFAVVLFISLAFLAAGAFSLYRVRMRKGA"
     gene            complement(4891..5814)
                     /locus_tag="SCJ86_001681"
     CDS             complement(4891..5814)
                     /locus_tag="SCJ86_001681"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307988.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pectin esterase"
                     /protein_id="HEG1169222.1"
                     /translation="MRMKDKLIIGKNAIDTPTDFETIQEGIDYLAGLPDSHPKVLIVK
                     EGRYDEYIESRLSNFKLVGAGTVVISGNRFAKQEYLDGSQLGTFRSATFFLEGSNVVL
                     ENLHIENTAGPGEKVGQAVALFNSGDRVSLKNCRLSGYQDTLCTGPLPDLQKDGTPFC
                     TPASANPEYCRQTYEYCWIEGTVDFIFGGADAVFHQCHIHSLASKNPGYITAASTPKN
                     QKTGYRFESCTLTAEPDTRNVYLGRPWRPYANVLFEACKIGGHVHPTGWDNWDDKKNE
                     QTVKFSERNNNYETSIKRPEWIHLEKQGVQK"
     gene            complement(5811..6689)
                     /locus_tag="SCJ86_001682"
     CDS             complement(5811..6689)
                     /locus_tag="SCJ86_001682"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016627941.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prolyl oligopeptidase family serine peptidase"
                     /protein_id="HEG1169223.1"
                     /translation="MQLNDFLGAPSSRHTPSGKRLYREKQNGFMLEYYQLYLNELETV
                     PALYAYPEKEGPFPTVLYLHSHGGDFSVGKSELIHGAPYLASPSFAEVLTGMGYAVWS
                     IDAWGFEERGGISESELFKQFLLTGKTLWGMRIYDVISLIDYLETREDTDTQRLATIG
                     LSMGGLLSWWVTALDSRVKVCIDLAAQVDIETLLLHRRLDHHGFYYYVPNLLTAYTTL
                     AIQEKIAPRPRLSLVGKNDTMCLDEGVLKLRKGLDKKYQSMGSPENFSSFSLTGGHME
                     TQEMRNIWKQFIREHL"
     gene            complement(6676..8013)
                     /locus_tag="SCJ86_001683"
     CDS             complement(6676..8013)
                     /locus_tag="SCJ86_001683"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324619.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 28 protein"
                     /protein_id="HEG1169224.1"
                     /translation="MYDILTFGASIDELNTEAIQQAIDAAASDGGGTVVVPAGEFLTG
                     ALFLKSNVELHLSAGAVLKFSDDPKDYPVVHSRWEGVHRKVYASCIYAQNVENISVTG
                     FGTLDGNGKKWWHTFRNEPDNLAYPRPKLMSFHNCHRITVKDIKLIQSPSWTINPILC
                     SNATFDNLTILNPADSPNTDGIDPESCKNVRISNCHIDVGDDCIAIKAGTEDTYERIA
                     CENITITNCTMVHGHGGVVLGSEMSGSIRNITISNCIFQETDRGIRLKSRRGRGGIVE
                     DIRVSNIVMDNVMCPFILNLYYFCGPRGKEPYVWEKKAYPIDERTSAFRRIHFSNITA
                     RNVHASAGFIYGLAEQFIQEITFDNIDVSMAQDAVPGVPAMMTGIEEMSRQGFYIGFA
                     GKVSFNQMSIENHEGPAFLLENTKEISIEKFQSNQSENMDELVVRKNFAYAVE"
     gene            complement(8016..9119)
                     /locus_tag="SCJ86_001684"
     CDS             complement(8016..9119)
                     /locus_tag="SCJ86_001684"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307984.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 88 protein"
                     /protein_id="HEG1169225.1"
                     /translation="MENWSKKLAKSIMERTPRLYEEKWYKGKWSYDYGVVLKGFQLLW
                     ERTQEKIYFDFIKDNIDYFVQEDGTIRGYSVEEYNIDHVNTGKLFFLLYKETGEEKYK
                     KAAELLSQQLANHPRTSEGAFWHKEIYPYQIWLDGLYMGSPFYAEFIQNFSEEKDYQD
                     VLRQFEISYKHLFDPKTNLLIHAWDEKKVQPWADPKTGLSHHFWSRSIGWYLMAAADT
                     YELLHEETDCSLLKEILEKTLAALLPYQEENSGTWYQVTTETKRKGNYLEASGSSMFV
                     YAMAKGIRLGLLNKEEWLPQVKKSHQGLLDEFVLETKEGWLNLNKNCQVAGLGGADQR
                     DGSYAYYISEPIICNDQKGVGAFLQALIEVEAL"
     gene            complement(9193..10701)
                     /locus_tag="SCJ86_001685"
     CDS             complement(9193..10701)
                     /locus_tag="SCJ86_001685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286015.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HEG1169226.1"
                     /translation="MKKATLVKLGLSFAGILALAACGSSSSNSSSETEDGKAKITWLN
                     ILHHASPPTDTIVNLIEEKTDTELTFNWIPDASKEERLTTALASDELGDIVTLQIAML
                     KNSSVRNALKSGVFWDVSEYLKDYKNLSQISEDRIEAASIDGALYGVPIQKDYARAGL
                     VIRKDWLDNLGLDVPTTMDELYEVAKQFTENDPDGNGVDDTVGFGDRAPSDIRYTSFK
                     LFTSYFGAPNGWAVDDNGKFTPEFDTEEYMKALNFSRDLFENGYLAQDFAVTQKTDQQ
                     EQFAQGRTGIYTGMVDIKNLKTMGQGIQDDMELVPVNKISDGEGGGYHVWSEGNGIGG
                     LLAFPKSQVKDEAQLKKLLQFVDDLLEEDVYKLMTGGIEGTHYELNDDGSIEFIDMDL
                     WQQEVQPLSSSRPSEVTFSFKDTDPEKEFSNQLIKENEEFAVMDPSVPLDSPTNNEVG
                     SEIEKIVIDATVQYIMGQLDEDGFKKAVEDWKAQGGDRITQEYEEAYKAAAQ"
     gene            complement(10667..11359)
                     /locus_tag="SCJ86_001686"
     CDS             complement(10667..11359)
                     /locus_tag="SCJ86_001686"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338144.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF624 domain-containing protein"
                     /protein_id="HEG1169227.1"
                     /translation="MLQSLDKINGRLNRILQLVYLNFLWGGLVIAGLGVFTFGPASYA
                     MVAVIRKWLRSKETFPLTKTYFTYFKENYRETLLMSWIYGAIGIVLLVDLSFIHNWYL
                     RIALYIAAFIYVLSALYIFPIMAHYDWKGYFFKMKMAFLFGFSHLHYSLVLILSMVGI
                     YLMIIQTMPGVLTFIGGSLFFSAVTWTANSIFCHLEWKNSYTEKTEENKNIIKTVKEK
                     IYEKSNARKIRS"
     gene            complement(11381..12355)
                     /locus_tag="SCJ86_001687"
     CDS             complement(11381..12355)
                     /locus_tag="SCJ86_001687"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HEG1169228.1"
                     /translation="MESVKKQEPFQLTLSKKEEKLRRRKKIIAGIKTNKFLYVMILPG
                     VLYFLIFRYLPMGGLVIAFQDYQPFLGIMGSPWVGFKHFIRLFTEPTFFTLLRNTLIL
                     FGLNIFVFFPMPIILSLLLNEVTNKRLKNGIQTIIYIPHFMSWVIIVSITYVFLNVDG
                     GVLNEFLATMGFEKISFLTSPEWTRTVYIGQVIWKELGWSTIIYLSAITAVDTQLYEA
                     AEMDGAGRLRKTWHVTLPAIRPVIITLLILKIGQTLDLGFEHMYLLLNSLNRSVAEIF
                     DTYIYTAGLKNGQLSFSTAMGLFKGVVGLFMVILANKIAKKLGEDGVY"
     gene            12655..13527
                     /locus_tag="SCJ86_001688"
     CDS             12655..13527
                     /locus_tag="SCJ86_001688"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343344.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HEG1169229.1"
                     /translation="MKKKVSIGERSFTLFNNTFLILLALICIVPFLNIIATSFASTQE
                     VVAKKFILFPTTFSLDAYRYILSTPTIFRALAVSIGVTGVGTIVSMCATSLMAYGLSR
                     KYLFGRGFVNFLVVFSMLFSGGMIPTFLVVRSLGLVNSYWAMILPVAVNAMNMIIMRN
                     FFQALPDSLEESAKMDGCTDFGVFFKIMLPLALPSIATISLFYAVTYWNTYMTAILYI
                     NDSSKWPIQILLRQIVIVSSGMQAESSAVDVIPPAQTVKMAVIVIATVPMLIVYPFVQ
                     KYFVKGALVGSVKG"
     gene            complement(14109..16307)
                     /locus_tag="SCJ86_001689"
     CDS             complement(14109..16307)
                     /locus_tag="SCJ86_001689"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325599.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HEG1169230.1"
                     /translation="MKKSTVFYKIFLPMFLLGAVLVIGFSLFIYQNTYESIESSYLTD
                     KKNLLRQVKTNVEWKIRTIEYSFSTYGSTKNFSDIFKHPLTYTDYSTYSEIRKELNFI
                     ETIVMDDNNYDLVSLAGKWGVINGSLSQLTEEEVEKYQQKYINNKNNLFWQKKNKGIE
                     MVISLPMFEKEKYALGIATIEKHTIEQVVGESGEDILSIENEVGNLFTTQTVKNKEQP
                     TVLTDVDLDYNQLKVIREKDKTYIVLKSDYNNWIYRLEVDSSAIGAMIRNLRIGLVAV
                     SLTLVGLIGLFSYLFSDRFARPISQIQERLNLKSKGFSGKELDLVAQSVSRIIGEKEA
                     LSAHLVTQKPQLETLFVLSLFRNRVERRELDQRLQQFGYSSQNDCYYTALVQIDLLED
                     SHGGERDLLLLAINNMITEIVPKENRMLPIVLNEEMQATIYRMDKDDAEASKKVMEYC
                     RLIQKRVKEYLNLTISIGISNRFETLNESKQSVDRAKEALYYRVNTGPSSIIFYQEIV
                     PAAHEKTLIRYPIEEQNRLFEAIRSGNQEVKNLVHELIDALFAQNKNPLSREVVSVRL
                     INELVQLGQLLGVDSKNFDNMKQIYVKALNNYHPKELEKLVLEQLVLPITECSQAITD
                     HEFKNLSENIMHIVYTEYDRDLSLDVIADRLHYNPNYLSNVFKKETGEKFGDFVQNHR
                     LEVAKKWLKETNLSVKEIAERLQYRNPQNFIRFFKKKESITPGEYRKKYL"
     gene            16539..17531
                     /locus_tag="SCJ86_001690"
     CDS             16539..17531
                     /locus_tag="SCJ86_001690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304853.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HEG1169231.1"
                     /translation="MVTIKDIAKAAGVSHTTVSRALNNSSLIKNETKQKIRQLAKEMN
                     YSPNYSAKGLVNQKKYLIGLFFSSLHKGTSSNFLADSIAGIHSVLDTNYSLSVESIDV
                     VDLTEINLQRYDGLIVMSQSDDDQPFIDYLKEKEFPFVVVNRHINDPAVINVTADDAA
                     GVMQAVDYGIGCGHTKIAYIGGKKNFRSTKEREKGVLKSLKKASIPVNLAYFLTGDYS
                     LESGLQNMEYLLSLSDLPTLVFCGNDDIAIGALRAVHLAGLRVPQNISLIGFDDSPVV
                     SYLNPPLTTVRKPLKEMCQEGTKLLLDLIEGRTIEQSKIELSTLLILRESINYL"
     gene            17591..19012
                     /locus_tag="SCJ86_001691"
     CDS             17591..19012
                     /locus_tag="SCJ86_001691"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286004.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tagaturonate reductase"
                     /protein_id="HEG1169232.1"
                     /translation="MKKLTKELVKKNEAPVKVLQFGEGNFMRGFIDWQIQQLNNQQLF
                     KGNVAIIQPLEQGLGNMMNQQDNLYTVILQGLSNGEIVDTSEIITVVEQMINPYDEWD
                     AYLSLAENDDLAYIVSNTTEAGIQFVETDNLTDTPPQSFPGKLTAFLYRRFKLNKAGF
                     TIIPCELIDRNGERLKEIILRYAEKWDLEPAFVEWLEKENVFCCSLVDRIVPGYPRED
                     AGVFNQRHNYEDQLMVKAEPFMLWVIEGPKELENQLPFKQAGLNVIITDDMTPYRERK
                     VHLLNGPHTAMVPLALLAELETVEEVMKDPLFSAYVDQLFNLELIPMLSLPKDELAIY
                     ADQIKERFLNPFAHHKLEAISLNSVSKFSTRLLPVFKKYIEEQNQVPPLITVSLAALL
                     LMYRGDQVKPHDDEKTISEFTDAWSDNGTAIPRLLQNAALWGEDLSQIPNVTDTVQEI
                     AAKIDQKGVRSVLQEMKGTMVHA"
     gene            19005..20504
                     /locus_tag="SCJ86_001692"
     CDS             19005..20504
                     /locus_tag="SCJ86_001692"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310848.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="altronate dehydratase"
                     /protein_id="HEG1169233.1"
                     /translation="MLETLQPTMKLNRRDSVVVALTPIPKQTQLTIDQQTITTLEDIP
                     QGHKIALGDLKAGDNVIKYGYPIGHVTTDVTAGQWLHTHNVKTNLSGELDYRYEKDVH
                     PSHYLFENRTFQGYLRKNGKVGIRNDLFLVPTVGCVNGIAELIVKQFKEKHPDLGQFD
                     HITILKHPYGCSQLGKDHQNTREILADAVNHPNAGGVLVFGLGCENNTVPEFKKILGE
                     YDEERVKFLVAQDVYDEIEQGVALLEELLTAAENDQRISVPLSKLNVGLKCGGSDGLS
                     GITANPLLGAFSDYLIAQGGSTILTEVPEMFGAEQVLMARAENEEVFDSIVDLINDFK
                     QYFLSYGEPVYENPSPGNKAGGITTLEDKSLGCTQKSGSSPVVDVLQYGEKIRKPGLS
                     LLQAPGNDLVAASALASSDCQLVLFTTGRGTPFGSYVPTVKVSTNTTLFERKGHWMDF
                     NAGVLLEQPMEVILESFVQKIIAVASGEETKNEQNDVREIAIFKNGVTL"
     gene            20543..21937
                     /gene="uxaC"
                     /locus_tag="SCJ86_001693"
     CDS             20543..21937
                     /gene="uxaC"
                     /locus_tag="SCJ86_001693"
                     /EC_number="5.3.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628919.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucuronate isomerase"
                     /protein_id="HEG1169234.1"
                     /translation="MFLSDDFLLKDDWAKKLYHSYAKNMPIIDYHCHLDPKEIYENNN
                     FSNLTEAWLGGDHYKWRLMRACGVPEEKITGNASDFEKFLAWCQTVPKIIGNPLYSWS
                     HLELKRFFGIDLLINEENAEKIWTQANEILAAPDFRRREIAKKSNVEVICTTDDPADD
                     LHYHQLLAHEEPDLKVLPSFRPDKALNIEKEGFNAWVTLLEEAADQTITTYQELIEVL
                     GQRIEYFHQHGCRVSDHALDILRYKEADETVLEEIFQKARANKLLSSDEIDAYLTETL
                     IHLTHFYHSHNWTMQLHIHAYRNCNTQMLEKLGPDTGYDGMNDLSLTIPLQKLLNRAE
                     ETDQLPKTILYSLNPNDYPAILALMGCFQKEQNGKLQLGSGWWYNDTRAGMRDQLTQF
                     ADGSALGNFVGMLTDSRSFLSYTRHEYFRRVLCEFLGEMVMRGEAPEDEQLLGSLVQA
                     ISYTNAKNYFGFFA"
BASE COUNT         6670 a         4494 c         3823 g         7043 t
ORIGIN      
        1 atcattaaaa aaaataaagg tcaagctaaa atattattta agcttgacct tattataatt
       61 tcaacgattg tcaaaagtat tgtttgcagc tgtgttgcca gtttctatag ataaatcaca
      121 gcttttgaaa cataagtttt gtactgcttc gattcgcccg aattcttttg cagttatttg
      181 gcaatgagaa aagcagacat cttctatagg cttttcagga aatgctacta aatcaaaagc
      241 tcgggaagga agtgagtaac ctggagaaag gcatgcctgt acgtttttga tgtgtatgtc
      301 tttgactttc gtcatttgct ttgattcagg tatttcttca gccactgctt gccaactttc
      361 tggcatttga gcaaggtcat tgctattttt tctattgtat tcattatgcc agtccattat
      421 ccaagaaaaa ggaaattgta catcaatcat attcagattt tctaccctga tattttcgat
      481 cactccccct ctttcttttg aagatttcat tcgaatacca cagtctgact gaaaaaaatt
      541 gatatcgtga atatatacgt cactaactcc agcactaact tcgctaccaa tagttacacc
      601 gtatcctgaa cggattttac atctagcaac ctcgattctt gatgattttc gattgactct
      661 tgcaccattg ccatctctgc cagacttaat agcaatacaa tcatcaccac aggccaaatc
      721 acattcatag acacgcacat ttgttgaaga atcaatatca atcccatctg tacttggtcc
      781 atcattgttg cggatgatta ctttttctac taatacatcg ttagaataag tgatttgcaa
      841 gttccaaaaa cctgatttct gaaaaatcaa atcacgaaca acaacatgtt ctgcatgata
      901 cagtaaacaa gcgcgtggac gtttaatagc ataatccgca atccaacgaa gtcccttttg
      961 atcgtattca gctcttgtcc cgcttttttg atcttttccc caatacagct cccaccaatg
     1021 gtctccttga ccgtcgatta ttccttttcc ttctataaga atatctttcg ccgagagaac
     1081 attcaaaatc gctgctggcc atttcatttc tacgccggct actcgtgtat cgatttctgg
     1141 aaatgcttcg atatctgttc tccccttgag gactgcacct tcttcaaaga taagatgact
     1201 cttatctttt aaaaaaaggg ctccgactaa ataaatgcct tttggaataa atacaggtaa
     1261 gtgttctttc gcaccttgat cgattgcttt ttgcagtatt attgtatcat cttgttgagt
     1321 gtcgtttgtt ttaaagttca aagcattgat aatccctttt tttgtccgca atacgtttct
     1381 ccctatcttt cattgatttt tttcaattct tcaccaataa atacagtttt tgaacgattc
     1441 agtgggtaac ctactagcaa aatgattgcg gatacaaata agcagaatgc tggtaacaag
     1501 tttgccatcg tataaatcct ttcttgtact aaaactgatt ggatggctcc acctgttgta
     1561 gacgattggt aaccaatgag tgccagcata aatccgccta ccgctcctgc acatgcttgt
     1621 cctacttttc tcgcaaaaga atttactcca tatactgttc catcttctct taagcctgtc
     1681 ttatattgat gataatcaat cacgtcagta atgaatgccc agaccatcaa attgaataaa
     1741 ccagatccta aagtagcgat aaataaaaga atcagatata cccatgcatt tgttatatga
     1801 aggaaataca tcagcaagta catacatgaa gaagcaagta acgcaaatac gcttgcttcc
     1861 tttttaccaa ataattttgt cagctttgta gcaaatggtg ctaaaactac tactgttccg
     1921 taagtaaata aaagagcaac agacattgct tgtttattat ggaagtaatc attgaataaa
     1981 taagtcatat tcgtcccagc taaaatctga ttgatcacaa tgaaaatgtc aacaatgact
     2041 aaaataatca gtgctcgatt agctaacaaa ccttttacca actgagaagc agatactttt
     2101 tcgtttttga caactcttac tctttcagta gtcaatttac aagttaacat atatgcgaca
     2161 aatgctattg cagcacatcc gattgaaatc caaaagaaat gattccctga aacgacttgt
     2221 tgtccactct tgttatgggt atacattaaa atcggaatga agaatcctgt agttgatccg
     2281 ccaatcgcag aaccaatgct tcggaacgtg gacaacgagg tgcggtcatt aggatttgag
     2341 ctaattgctg ctgccattgc tccatacggg atattgattg ttgagagaaa aatcccataa
     2401 acaatgtatg aaacaaaaat ataagcaatc ttcaccgtca ttggaagatg ttggacgaat
     2461 ggcaaaaaca aaatgacagt cactaagcaa aatggatact tcatttttct gatccaagga
     2521 ataaatcggc cagtttctgt taactctgag acatcacata atcttccaac catcacatca
     2581 gcaaacgcat ccaaaattct cgaaatcaag aataacgtac ctacgattgc ccctgaaacg
     2641 ccaagaacat tcgtataata aatcatcaag aaactattga ctaaacctaa aataaaacag
     2701 ttacctagat caccgaacat gtagcctatc ttatcggaaa tcccaaattc ctttttttct
     2761 ttttttactg tatttgctgt aacagcattt tgtgaaagca tttgaattcc tcctacctct
     2821 tctagtaaac gttttcaaaa ttataactct ttttttcttg tttttatatt cacaatgttg
     2881 gctttttatt gcattatctt gacagataaa aaacaaaaaa taatctaaaa acattgatgc
     2941 ataagattga taactttttt tacataacaa tatatacaaa caaaaaaatt cactaactag
     3001 tgaaaataag ttagtgaacc tcatttatta aagaattaaa ttacagatga tcttttccat
     3061 catacttgaa aaactcaaaa tcagcataac ttcctctttt tttatttaga tcatgcactg
     3121 ctatcccgat aaaatttcca gtaaaccctc ctgagagtat acacatgttt ttttgcggac
     3181 ctactttctt ccattcttca gttgatacgt ctttcaaatg aaactgtgct gtctctttct
     3241 ttacagtgac tttcaaaaaa gtcttctttt ctgtgagtgg aatcgtccaa tcttctattt
     3301 gtgctactcc gttttctgat tctatcatac gtaggcattt tcccttgtcc tcattccaag
     3361 taacataaca ataaatataa ttggattcat ttaagtacag taccagtcca gccatctgat
     3421 taaaattatt aggcatgtat tcaaatgctg tctccgcatc aaaagtaaaa tccgtttgcc
     3481 gaatagctaa caaatgatgt tcgaaggtag attgaagaga ttcaccggaa atcagacgta
     3541 aaaaacctgg tctacatttt gtatcacacc aatcagcatt tggcaaaatg cgtctggcat
     3601 tccagttctt ctttagctct ccttcaaatg tatccataaa ctgatgatct ttttcttgtt
     3661 gtacctcttc atttgtctca atcattattt cttccatcgg agttgtctgg ctatctttaa
     3721 gtcttaacca atcgtcggac gtccaataca cttcttgaat agcggtttct cttcccaaaa
     3781 tagccgcacc gtttaatggt ctggtacaaa gatatgtcat gtaccatctt cctgacgggg
     3841 tttgtactaa gctcccatgt cctgtacatt gcaatgtaga agcaggtttg tcactagctg
     3901 tcagcatagg aaaaccagga tcaagctcgt aaggaccagt aatattcttt gctcggcaaa
     3961 ctgtaactga atgtcctctt cctgtaccgc cttctgccgt catcaaataa taatacccgt
     4021 tacgaaaata caaatgtggt gcctccgttt tcgctaattc tgtacctgca aatattttat
     4081 agatgggacc atcaagcttc ttcttctctg catcatattc ttgtaatacg ataccgctgg
     4141 atttattcgg tgtgtccaga cgataatccc atagttcatt gacaagccat ttttttcccg
     4201 tttcaagatc atggaacaaa gaaggatcaa agccgttact atttaaataa attggttcgg
     4261 accaaggacc tgtgatctca gatgcagtga tcaaataatt gtggacatct ttaaacggac
     4321 ggcctgcact tttgacatct gtgtaaacaa gataaaacaa tccatcagca taactgatct
     4381 gtggcgccca tatacttcca tttgtaggat ttccttgcaa agatacttga gtggtcaaaa
     4441 tatccgttgt atgtgaccag ttgactaaat ttttcgactc atatactcgg attcctggaa
     4501 gccattcaaa agaggagaca ataatgtaat accgctcttt cactcttaat atattaggat
     4561 ctggattgaa tcctcttaaa ataggatttt tcactttgat agtctgcatg attcaggctc
     4621 cttttctcat tcttactcga tataaactga atgccccagc tgctaaaaaa gcaagtgaaa
     4681 taaacaacac aacggcaaac agaccagtca tgtcctgtaa taatccaaat gtattttcta
     4741 aagacaaagt tgaatgatct acagcaagcg actgataact ttttttaaac tctgacgcag
     4801 acatattact taaagtaact gctaacccgc caaagaaaat caaagataga ctgccaccga
     4861 ccattcctag caatagttcc cattttcttt tcatttctga accccctgct tttctaaatg
     4921 tatccattca ggtcttttga tcgatgtttc ataattgtta ttacgttcag agaatttgac
     4981 agtctgttca tttttcttgt catcccaatt atcccaacca gttgggtgaa catgcccacc
     5041 tatcttacac gcttcaaata agacatttgc atacggacgc cacggtcttc ctaaatagac
     5101 gtttctagta tcaggttctg cagtcaaggt acagctttca aaacgatacc ctgttttttg
     5161 atttttcggc gtagatgctg ctgtgatgta tccaggattt ttactggcta agctatggat
     5221 atgacactga tgaaaaacgg catctgcccc accaaatatg aaatccaccg ttccttcaat
     5281 ccagcaatat tcataggtct gacgacaata ctcaggattg gcagaagccg gtgtacaaaa
     5341 aggagtacca tctttttgca aatcaggcaa cggacccgtg cataaggtat cctgataacc
     5401 agatagtcga cagtttttta aagatacgcg atctcctgaa ttgaataaag caacagcctg
     5461 tcctaccttt tctcctggac ctgctgtatt ttcgatgtga agattttcta atacgacatt
     5521 cgatccttct aagaaaaaag tagcacttcg gaaagtccct aactggctac cgtcaagata
     5581 ctcctgtttt gcaaaacgat taccggagat aactactgtt cctgctccca caagtttaaa
     5641 attggataat ctgctttcta tatattcatc atatcttcct tcttttacta tcaagacctt
     5701 tggatggcta tccggcaggc ctgccaaata atcgattcct tcttggatag tttcaaaatc
     5761 cgtcggggta tctatagcat tctttccaat tatcagtttg tctttcatcc tcataaatgc
     5821 tcccttatga attgtttcca aatatttctc atttcttgtg tttccatatg accgcctgtt
     5881 aaagaaaaag aagaaaagtt ttctggacta cccattgatt gatacttttt gtccagccct
     5941 tttctcagct tcagtacgcc ttcgtccaag cacatcgtat cgttttttcc aactaagcta
     6001 agacgagggc gcggagcgat tttttcttgg attgctaacg ttgtataagc tgtcaataga
     6061 tttggcacat agtaataaaa cccatgatga tctaggcggc gatgtagcaa aagtgtttcg
     6121 atgtccacct gtgccgctag atcaatacaa actttgacac gagaatccaa tgctgtcacc
     6181 caccagctta ataagcctcc catagaaaga ccaatcgttg cgagccgttg agtatctgtg
     6241 tcttcccttg tctccaaata atcgatcaag ctgatcacat cataaatacg cattccccat
     6301 aacgtttttc ctgttaataa aaattgtttg aacaactcgc tttcagaaat acctccgcgt
     6361 tcttcaaatc cccaagcatc aatcgaccaa acagcatatc ccattcctgt cagtacttct
     6421 gcaaaagatg gagaagctaa gtaaggagca ccatggatca attcactttt cccgactgaa
     6481 aaatctccgc cgtgagaatg caggtaaagg accgtcggaa acggtccttc tttttctgga
     6541 taagcataga gggctggtac tgtttccaat tcatttaagt acaattgata gtattcaagc
     6601 ataaaaccgt tttgtttttc tcggtataat cgttttccac taggggtatg tctgctagat
     6661 ggagcaccta aaaaatcatt caactgcata agcaaagttt ttacgaacaa ccagttcatc
     6721 catattttct gattgatttg attggaattt ctcaatactg atttctttag tattttctaa
     6781 tagaaatgct ggtccctcgt gattttcaat cgacatctgg ttaaaggaaa ccttacccgc
     6841 aaagccaata taaaatcctt gtctgctcat ttcctctatt cctgtcatca tagcaggaac
     6901 accgggaact gcatcttggg ccatcgagac atcgatattg tcaaatgtga tttcttgtat
     6961 gaattgttcc gctaatccat aaataaaacc tgcagaagca tggacattcc ttgccgtgat
     7021 attcgaaaaa tggatccttc tgaatgctga tgtccgttca tctatcggat aagccttttt
     7081 ttcccataca tacggttctt tccctcgcgg accacagaaa taataaagat tcagtataaa
     7141 tggacacatc acattatcca tgactatatt ggatactcga atatcttcaa cgatcccgcc
     7201 tcgacctcgt ctagatttca atcgtattcc tcgatcagtt tcttggaaga tacaatttga
     7261 aatggtaata ttacgaatac taccactcat ttcacttcct aatacgacac cgccatgtcc
     7321 atggaccatc gtgcaatttg tgatcgtgat attttcgcaa gcgatccttt cgtacgtatc
     7381 ttcagtccct gccttgattg caatacagtc atctcccacg tcaatatggc aattactaat
     7441 acgcacattt ttgcatgatt ctggatcaat tccgtctgta tttggtgaat ccgctgggtt
     7501 caagatcgtc aagttatcaa acgttgcatt actgcaaaga atcgggttga tcgtccaact
     7561 tggtgactgg atcaatttaa tatctttcac agtaattcga tgacagttat ggaaactcat
     7621 caatttagga cgaggataag cgagattatc tggttcattt ctaaaagtat gccaccattt
     7681 ttttccattt ccatctaatg taccgaaacc agtgacagag atgttctcta cattttgagc
     7741 atagatacag gaagcataaa cttttcgatg gactccttcc caacgtgaat gaacaacagg
     7801 ataatctttt ggatcgtcag agaatttcag aaccgctcct gcgcttaaat gaagttcgac
     7861 attgcttttt aaaaataaag ctcctgttag aaattcacct gctggaacaa ctacggtccc
     7921 gcctccgtca cttgctgcag catcaatggc ttgttgtatt gcttcagtat tcaattcatc
     7981 aattgatgcg ccaaatgtca aaatatcata catatttata atgcctccac ttcaatcaat
     8041 gcttgcaaga atgcaccaac gcctttttga tcattacaaa taattggttc actgatgtag
     8101 taagcatatg aaccatctcg ctgatctgct ccgcctaacc ctgctacttg acagttttta
     8161 ttcaagttca gccagccttc ttttgtttcc agaacaaatt catcaagtaa tccttgatgt
     8221 gatttcttca cttgtggaag ccattcttct ttgttcagca gtcccaaacg tattccttta
     8281 gccattgcgt aaacaaacat actactgccg gaagcttcca gataatttcc tttccgtttt
     8341 gtttctgttg ttacttgata ccatgtcccg ctgttttctt cttgatatgg aagtaatgca
     8401 gccagtgtct tttctagaat ttctttcagc aaagaacaat ctgtttcttc atgaagtaat
     8461 tcataggtat ctgctgcagc catcaaatac cagccaatcg atcggctcca aaaatgatgg
     8521 gatagacctg tttttggatc agcccatggt tggaccttct tttcatccca tgcatgaatc
     8581 agcaaatttg tttttggatc gaatagatgt ttataactga tttcaaactg tctcaaaaca
     8641 tcttggtaat ctttttcttc actaaaattc tggataaatt cagcataaaa aggagaaccc
     8701 atatataagc catccaacca aatttgataa ggataaattt ctttatgcca aaaagctcct
     8761 tcggatgtgc gtggatggtt tgccaactgc tgggaaagga gttctgctgc ttttttatat
     8821 ttttcttcgc ctgtttcttt gtacaataaa aagaaaagct ttcctgtatt tacatggtcg
     8881 atattgtatt cttcaacgct gtagccacgg atcgttccat cttcttgcac gaaatagtcg
     8941 atattgtctt ttatgaaatc aaaataaatt ttttcttggg ttcgttccca aagtaattgg
     9001 aaccctttaa gtacgacgcc atagtcataa gaccattttc ctttatacca cttttcttca
     9061 tataagcgag gtgttctttc catgatactt tttgctaatt tctttgacca gttttccatt
     9121 atttgctctc ctttaaaaat taaaatagga tttgggacaa ataccaagaa gtatttgtcc
     9181 caatcgtcta tattactgtg ccgctgcttt gtatgcttcc tcgtactctt gtgtgattcg
     9241 atcgcctcct tgtgctttcc agtcttctac agcttttttg aatccatctt cgtcaagctg
     9301 tcccatgatg tattgcaccg tagcatcaat gacgattttt tcaatttctg atcctacttc
     9361 attgttcgtc ggtgaatcca atggcacaga aggatccatt acagcaaatt cttcattttc
     9421 tttgatcaat tgattagaga attctttttc agggtctgta tctttgaaag agaacgtcac
     9481 ttcactagga cgagagcttg ataacggttg cacctcttgt tgccaaagat ccatatcgat
     9541 aaattcgata gaaccatcat cattcaattc ataatgcgtt ccttcgatac cgccagtcat
     9601 caatttatac acatcttctt ctaaaagatc atcaacaaat tgaagtaatt tcttcaattg
     9661 tgcttcgtct tttacttgag atttgggaaa tgctagcaag ccgccaatac catttccttc
     9721 tgaccaaaca tgataaccgc cgccttcacc atctgagatc ttgtttacag gcactaattc
     9781 catatcatct tgaatacctt gacccatcgt cttcagattc ttgatatcca ccattccagt
     9841 gtagattcca gttcttcctt gtgcaaattg ttcttgctga tcagtctttt gggtaactgc
     9901 aaagtcttgt gccaaatagc cattctcaaa tagatctctt gaaaaattca aagctttcat
     9961 atactcttct gtatcaaatt ctggagtgaa tttgccatta tcgtctacag cccagccatt
    10021 tggtgctcca aaataagaag tgaaaagttt aaagctggta taacgaatat cactaggcgc
    10081 acgatcgcca aaaccaactg tgtcgtccac accatttcca tcaggatcgt tttctgtaaa
    10141 ttgtttagct acttcataaa gctcatccat ggtagttgga acatctagtc ctagattatc
    10201 caaccagtct tttcgaatga ctaagccagc acgagcgtag tctttttgta tcggtacgcc
    10261 gtacaaggca ccgtcgatac tcgcagcttc gattctatct tcagaaattt gacttaaatt
    10321 tttatagtct tttaaatatt ctgatacatc ccagaatacg cctgatttta acgcattgcg
    10381 gacagaagag tttttcaaca tcgcaatttg aagtgtaacg atatctccta gttcatcaga
    10441 agccaatgca gttgttaatc tttcttcttt tgaagcatca ggaatccagt tgaatgtcag
    10501 ttccgtatcg gttttttctt cgatcaagtt cacaatcgtg tctgtaggcg gtgaagcatg
    10561 atgcaaaata tttaaccaag tgattttagc ttttccatct tctgtctctg aagaactgtt
    10621 acttgaactg ctgccgcatg cggcaagtgc tagaattcct gcaaaactaa gacctaattt
    10681 tacgagcgtt gcttttttca taaattttct ccttcactgt tttaataata tttttgtttt
    10741 cttctgtttt ttctgtatat gagttcttcc attccaaatg gcaaaatata gaatttgctg
    10801 tccacgtgac tgcggaaaag aagagactgc caccgataaa ggttaaaaca cctggcattg
    10861 tttggatgat catcagataa atccctacca tacttaatat caacactaat gagtaatgaa
    10921 gatgtgagaa tccgaataaa aatgccattt tcattttgaa aaaatatcct ttccaatcat
    10981 aatgagccat gattgggaaa atatagagtg ctgagagcac atatatgaac gcagcaatat
    11041 acaaagcgat cctaagatac caattgtgta tgaaagaaag atccacaagt aaaacaatcc
    11101 cgatggcgcc atatatccag ctcatcagca aggtttcacg atagttttct ttaaaatatg
    11161 tgaagtacgt tttcgtcaaa ggaaacgttt cttttgagcg cagccatttt ctaatcactg
    11221 caaccatcgc atacgatgca ggaccgaaag taaaaactcc caaacctgcg atgactaaac
    11281 cgccccacag aaaattcaaa taaaccaact gtaaaatacg atttagcctt ccgtttattt
    11341 tatctaatga ctgcagcata tctttccctc tctttctatc ttagtaaacg ccgtcttctc
    11401 ctagcttttt ggcaatctta ttagctaaaa ttaccataaa caatcctacg acccctttaa
    11461 acaatcccat tgctgtactg aaacttaact gtccattctt caatccagct gtataaatat
    11521 aggtgtcgaa aatttctgca acgcttcgat tcaatgaatt caataacaag tacatatgct
    11581 cgaaccctaa gtctaatgtc tgaccgattt ttagaatcag caaggtaatg attactggac
    11641 gaattgccgg caatgtaaca tgccatgttt tgcgtaagcg tcctgctcca tccatttctg
    11701 cagcttcata caactgtgta tcgactgcag ttatcgctga aaggtagatg atcgtagacc
    11761 agcccaactc tttccagatc acttgtccga tatatactgt gcgtgtccat tcaggggatg
    11821 ttagaaaact gattttttca aatcccattg ttgcaaggaa ttcattcaat acccctccgt
    11881 caacattcaa aaatacataa gtaatcgaaa caatgattac ccaagacatg aagtgaggga
    11941 tgtaaatgat cgtttgaatc ccgtttttca atcgtttgtt cgttacttca tttaatagta
    12001 aagacaaaat gattggcatc ggaaaaaaca cgaagatatt taatccgaac aagatcaacg
    12061 tatttcttaa taatgtaaag aatgtcggtt ctgtaaacaa acgaatgaaa tgtttgaaac
    12121 cgacccaagg acttcccatt attcctaaaa atggttgata atcttgaaaa gcaataacga
    12181 gtccccccat aggaagatac ctaaaaatca agaaataaag gactcccggc agaatcatca
    12241 catataaaaa tttattcgtt ttgattcctg caattatttt ttttcgtctt ctgagctttt
    12301 cttctttttt cgatagcgtc agttgaaatg gttcttgttt ctttacactt tccataagcc
    12361 cctccttagt tttgtgaatg tttgctgctt atgacttcat gatagataac tatctgaatt
    12421 acgtatataa tcatttttat cgtaagtaaa aacgcttaca tagcaatgat ttttgtgcta
    12481 tacaaaacga taaagtgtat tttttcacaa ataaactacg catagcaaac cttctatttt
    12541 tttctatctg acacttagca taggaaatga ttatatacgt aatctttcac cgatgataaa
    12601 ttgaacatat agaaacagaa acatttaatt tattttgagg aggagcctat ctatatgaaa
    12661 aagaaagtct ctataggaga aagaagtttt acacttttca acaatacgtt tctaattttg
    12721 ctagctttga tctgtatcgt tcctttttta aatatcatcg ctacatcttt tgcttctaca
    12781 caagaagtcg tcgcgaaaaa gtttatcctt tttcctacta ccttttcttt agacgcgtat
    12841 cgctacattc tttctacgcc gaccattttt agagcacttg ctgtttcgat tggtgttaca
    12901 ggtgtcggaa cgatcgtcag tatgtgtgca acttcactga tggcctacgg tctttccaga
    12961 aagtatttgt ttggtcgagg tttcgtcaac tttcttgttg tattttctat gttgttcagc
    13021 ggggggatga ttccaacgtt cttagttgtt cgttctctag ggctggtcaa ttcttactgg
    13081 gccatgatcc tgcctgtggc tgttaatgca atgaatatga tcattatgag aaactttttc
    13141 caagccttgc cggatagttt ggaagaatca gcaaaaatgg atggttgcac agattttggt
    13201 gtatttttca aaattatgct gccattagct ttaccgtcta ttgctactat ctcgcttttt
    13261 tatgctgtta cttactggaa cacttatatg actgctattt tgtatatcaa cgattcatct
    13321 aaatggccga tacagattct tttacgccaa atcgtcattg tatcaagtgg gatgcaagca
    13381 gaaagtagtg ccgttgatgt gattccacca gcacaaacag ttaaaatggc tgttatcgtt
    13441 attgcaacag tgcctatgct gattgtttat ccttttgttc agaaatattt tgtcaaagga
    13501 gcactcgtag gttctgtcaa agggtgatta tgtactgaac gcaaaatggt agttgtgtca
    13561 gaagtggtca gcttcaagaa ataagaaggc atttacgaaa attgcttctt aaatttttgt
    13621 gaaatttcgg cttatttttc gaagaatcta ctcctggaac accgtgtatc agaggttgtg
    13681 aaagagacgt tcagccttga gcgatgataa aaactctcac aaacattgcg acgagtaacc
    13741 cacaggagca ataagaagcg attttgaaaa atagcttccc atatttcaca aaattatgcc
    13801 ttattgtcga tagcctgtct cttgaacgcc atttattcgt aaaaaagaac tgtgacaaga
    13861 cttttgtcac agttcttttt gcgtgcttag ccttgaaatt taactatcca taaatacaaa
    13921 tactagggtt attctaactt cttttattgc ttgaatcagt ccacaagaaa aacagcctct
    13981 tgattaaacg gcgtttatct tgttgattct gcggcaataa aatcagccag cagcaaaaca
    14041 tgaagtgctg ttttttgctg tcctctttta ttgcttgaat cagatcacaa gaaaaacagc
    14101 ctcttgattc acaaatattt ttttcgatat tctcccggcg taatcgattc cttcttttta
    14161 aagaaacgaa taaaattttg cggatttcga tattgcaatc tttctgcaat ctcttttacg
    14221 gagaggttag tctctttcaa ccattttttt gctacctcta aacgatgatt ttgaacaaaa
    14281 tcaccaaatt tttctcctgt ttccttttta aatacattac ttaaatagtt agggttataa
    14341 tgcaacctat cagcgatcac atctaaagac agatcacgat catattctgt gtagacgata
    14401 tgcataatat tctccgacaa atttttaaat tcatgatcag tgattgcttg agagcattct
    14461 gtgattggca gaactaattg ttctaaaaca agtttttcta gttctttggg atgataattg
    14521 ttcagtgctt ttacataaat ttgtttcatg ttatcaaagt tttttgaatc tactcccaat
    14581 aactgtccta attgaacgag ttcattgatc aagcggaccg aaactacttc ccgactcaaa
    14641 ggatttttat tttgtgcaaa caatgcatca atcaattcat gtactaaatt cttcacttcc
    14701 tgatttccag aacgaatggc ttcaaacaat cgattttgtt cttctatcgg atatctaata
    14761 agtgtttttt catgagcagc aggaacgatt tcctgataaa aaataattga acttggacca
    14821 gtatttacac gataatacag ggcttctttt gcccgatcaa ccgattgctt gctttcattc
    14881 agcgtttcga accggttact gatgcctata ctgatagtta aattcaagta ttcttttaca
    14941 cgtttttgaa tgagtcggca atactccatg acttttttgc ttgcttctgc atcatcttta
    15001 tccattcgat aaatagtggc ttgcatttct tcattcaaaa cgatcggcag cattcgattt
    15061 tcctttggca caatttccgt gatcatgttg ttgatcgcta aaagtagcaa atcacgctcc
    15121 ccaccgtgac tatcttctaa cagatcgatt tgaaccaaag ctgtataata gcagtcattt
    15181 tgagaactat aaccaaattg ctgcaaccgc tgatctagtt ctcgcctttc tactcgattt
    15241 ctaaataaac taagtacaaa tagcgtttcc agctgtggct tttgggtaac taagtgtgca
    15301 cttaacgctt ctttttctcc tatgatccga ctgactgatt gagcgaccaa atctagttct
    15361 ttcccactga atccttttga tttaagattc agacgttcct gtatttgaga aatcggacgg
    15421 gcaaatctgt ccgaaaataa gtaggaaaaa agaccaatga gtcctaccag tgttaaagac
    15481 actgcaacta gcccgattct cagattgcgg atcattgcgc caattgcact agagtcaact
    15541 tctaatctgt aaatccaatt attataatct gatttcagta ctatatacgt tttatctttt
    15601 tctcttatca ctttcagctg attgtaatct agatccacgt ccgttaatac agttggttgc
    15661 tctttatttt ttactgtctg agtagtaaaa aggttgccaa cttcgttttc aatagaaagt
    15721 atgtcttctc cactttcacc aacaacttgt tctattgtat gtttctctat cgtagcaatt
    15781 cccaatgcat atttttcttt ttcaaacatt ggcaaagaaa tcaccatttc gattcctttg
    15841 tttttctttt gccaaaataa attatttttg ttatttatgt acttctgttg atacttttca
    15901 acttcctctt cggtcagttg actcaacgat ccattgatta ctccccattt accagccaaa
    15961 ctgactaaat cataattatt atcatccata acaatcgttt ctataaaatt tagttctttc
    16021 ctgatttcag aataagtgga gtaatctgta tatgtcagcg gatgcttgaa tatgtccgag
    16081 aaatttttgg tcgaaccata cgtagaaaac gaatattcaa tcgttctaat tttccattca
    16141 acgttagttt taacttgcct gagcaaattc tttttgtctg ttaaataact cgattcgatc
    16201 gattcatagg tattctggta aatgaataaa ctaaatccta tgacaagtac ggctcctaat
    16261 aaaaacatcg gtaaaaatat tttataaaat actgtacttt tcttcatttt cttcctctcc
    16321 tccttttaaa ataaaaggct ttcatttttc tgtaaaattg atactcatct aattaaatga
    16381 gcattccccg ctttcaagaa tagcataatt aaaaataaaa atccaagtaa tttttaacgt
    16441 gtgcaatctt ttcatatcaa agatctattg acttatcgta gttatcgtaa tatgatgaat
    16501 gcgttaacaa aaagtgaaaa agtaaggaaa atgatggaat ggtaacgatc aaagatatcg
    16561 ctaaagcagc tggcgtttct catacgacag tttctcgtgc tttgaataac agcagtttaa
    16621 taaaaaatga aacaaaacaa aaaatacgac aactagctaa ggaaatgaat tattcgccaa
    16681 actatagtgc aaaaggttta gtgaatcaaa aaaaatatct aatcggacta tttttctcaa
    16741 gtttgcataa aggaacttca tcaaatttcc tagcagattc cattgcaggc atccattcag
    16801 tattagacac aaattacagt ttatctgttg aaagtatcga tgttgttgat ttaacagaaa
    16861 tcaacctgca acgctacgat ggactgatcg ttatgagtca aagtgatgat gatcagcctt
    16921 tcatcgacta tctcaaagaa aaagaatttc ctttcgttgt tgtcaatcgt cacatcaatg
    16981 atccagcggt tatcaacgtc actgccgatg atgcggccgg tgttatgcag gcagtagatt
    17041 acggtattgg atgcggacat acgaagatcg cttatattgg cggaaagaaa aattttcgtt
    17101 cgacaaaaga acgggaaaaa ggggtattga aaagtctaaa aaaagcgagc atccctgtca
    17161 atctagccta ttttttaaca ggagattaca gcttagaaag cggcctccaa aacatggaat
    17221 acctattatc actttctgac ctgcctactc tggttttttg cggcaacgac gacatagcga
    17281 ttggtgcatt gagagctgta catttagctg gcctccgtgt tccccagaac atttctctga
    17341 tcgggttcga tgattctcca gtcgtctcct atctcaatcc gccgcttacc accgtacgta
    17401 agcctttgaa agaaatgtgt caagaaggaa caaagctttt actagatttg atcgaaggca
    17461 ggacgatcga acaatctaaa attgagctct ccaccttgtt gatccttcgt gagtcgatca
    17521 attacttgta aactacaagt aattttatat aaaaatgtta acgtgtgcat aacacagaaa
    17581 ggaagtttat atgaaaaaac tgacaaaaga attagtgaaa aaaaacgaag cacctgtaaa
    17641 agttcttcag tttggtgaag gcaattttat gcgtggattt attgattggc agattcagca
    17701 gctgaataac caacaattgt ttaaaggtaa tgttgcaatc attcaacctt tagaacaagg
    17761 tcttggaaat atgatgaacc agcaagataa tttatatacg gtcattttac aaggactcag
    17821 caatggcgaa atcgttgata cttccgagat tatcaccgta gtagaacaaa tgatcaatcc
    17881 ttatgatgaa tgggatgctt atctatctct agcagaaaat gacgacttgg cctatatcgt
    17941 ctccaataca acagaggcag gaatccaatt cgtggagact gataatttaa cagatactcc
    18001 cccacagagc ttcccgggca aactgacagc ctttttatac cgacgtttca aattaaacaa
    18061 agctggcttt accatcattc cttgcgaact gattgacaga aacggcgaac gtctaaaaga
    18121 aatcatttta cgctacgcag aaaaatggga tttagaacct gcttttgttg agtggttaga
    18181 aaaagaaaat gttttctgtt gtagtttagt tgatcgcatc gtcccaggtt atcctcgcga
    18241 agatgcaggt gttttcaatc aacggcataa ctatgaagat caattgatgg tcaaagcaga
    18301 accattcatg ctttgggtga tcgaagggcc taaagaattg gagaatcaac taccattcaa
    18361 acaggctggc ttgaatgtca tcatcacaga tgatatgact ccttatcgtg aacgtaaggt
    18421 gcatttgctt aatggaccgc atacagcgat ggtccctctt gctttgttgg cggaattgga
    18481 aactgtagaa gaagtaatga aagatccttt attctccgca tatgttgatc agttattcaa
    18541 tctagaattg attcctatgc tttctttacc taaagatgaa ttagccattt atgctgacca
    18601 aatcaaagaa cgtttcttga atccttttgc ccatcacaaa ttagaagcga tttcattaaa
    18661 tagtgtttca aagttcagca cccgtctgct tccagtgttc aagaagtata tcgaagaaca
    18721 aaatcaggta ccaccgttaa tcactgtttc actagctgcg ctacttctta tgtatcgagg
    18781 cgatcaagta aagccacatg atgatgaaaa gacgataagc gaatttactg atgcttggtc
    18841 tgataatgga acagctattc ctcgattatt gcaaaacgct gcgctttggg gagaagattt
    18901 gtctcaaata ccgaatgtga cagatacagt tcaagaaata gctgcaaaga tcgatcaaaa
    18961 aggtgtccgg tcagtattac aagaaatgaa aggaacgatg gtccatgctt gaaaccctgc
    19021 aaccaacaat gaaattaaat cgacgagaca gtgtggtcgt cgcattaacc cctattccaa
    19081 aacaaacaca actgacgatt gatcagcaaa cgatcaccac tcttgaagat atcccgcaag
    19141 gtcataagat tgcattgggt gatttgaaag ctggagacaa cgtgatcaaa tatggctatc
    19201 caatcggtca tgtgacgaca gacgtaactg ccggtcaatg gctgcacact cataatgtaa
    19261 aaaccaatct ttctggtgaa ttggattatc gatatgaaaa agatgtccat ccaagtcatt
    19321 acctttttga gaaccgaaca tttcaaggtt acttgcgaaa aaatggaaaa gtagggattc
    19381 gtaatgattt gtttttagta cctaccgttg ggtgtgtgaa tggcattgct gaactgatcg
    19441 tcaaacagtt caaggaaaaa catccagatc tgggacagtt tgaccatatt acgattttaa
    19501 agcatcctta tggctgttcg cagcttggaa aagatcatca aaatactcga gaaattttag
    19561 cggacgcagt aaatcatccc aatgccggag gagttttagt atttggatta ggttgtgaga
    19621 acaatacagt tcctgaattc aaaaaaattt taggtgaata tgatgaagaa cgagtgaaat
    19681 ttctagtggc tcaagatgtt tacgatgaga tcgaacaagg tgtggctcta ttagaagagc
    19741 ttttaacagc ggcggaaaat gaccagcgca tatcagtccc gctctctaag ttgaatgtcg
    19801 gtttgaaatg cggcggttct gatggtctat cgggaataac tgctaatcct ttacttggtg
    19861 cattttctga ctacttgatt gctcaaggag gcagcacgat cttaacagaa gtgccagaaa
    19921 tgtttggcgc agaacaagta ttgatggcac gagcggaaaa tgaagaagtt tttgactcaa
    19981 tcgttgactt gatcaatgac ttcaaacagt atttcttgtc ttacggggaa ccagtttacg
    20041 aaaacccatc tcctggtaat aaagcaggtg ggatcactac attagaagat aagtctttag
    20101 gctgcacgca aaaatcagga agctctcctg tcgttgatgt gctacaatat ggcgaaaaaa
    20161 tcagaaaacc agggcttagt ttattgcaag ctcccgggaa tgatcttgta gctgcctctg
    20221 ctcttgcttc atccgattgc cagttggtct tgtttaccac aggaagaggg actccgtttg
    20281 gctcatatgt cccaacggtc aaggtatcta cgaatacaac tctttttgaa cgcaaaggtc
    20341 attggatgga ttttaatgca ggcgtcttat tagaacaacc aatggaagta atcttagaat
    20401 cgtttgttca aaaaatcatc gctgtagcta gtggagaaga aacgaaaaat gaacaaaatg
    20461 acgttcgaga aattgccatt tttaaaaacg gagtaacact ttgattttca taaaacagta
    20521 taaaaagaaa gaaggaagat ttatgttttt atctgatgat tttttattaa aagacgattg
    20581 ggcgaaaaag ctttaccatt cctacgcaaa aaacatgccc attattgatt atcactgtca
    20641 cttagatcca aaagaaattt acgaaaacaa caatttcagc aatctgacag aagcttggtt
    20701 aggaggcgat cattacaaat ggcgcttgat gcgagcttgc ggcgtgcctg aagaaaaaat
    20761 cacaggaaat gcttctgatt ttgaaaaatt tttagcttgg tgtcaaaccg taccaaaaat
    20821 tatcggaaat ccgctttaca gttggtcaca tcttgaattg aagcgtttct tcggtattga
    20881 cttgttgata aatgaagaaa atgcagagaa aatttggaca caagcaaatg aaattttggc
    20941 agctcctgat tttcgccgtc gggaaatcgc aaaaaaatcg aatgtagaag tgatttgtac
    21001 aacagatgat cccgcagacg acttgcacta tcatcagttg cttgctcatg aagaaccaga
    21061 tttgaaggtt ctaccttcat tccgtccaga taaagcactc aatattgaaa aagaaggatt
    21121 taatgcatgg gtcacactac tggaagaagc agctgaccaa acgatcacca cctatcaaga
    21181 actgatagaa gtcttagggc agcggataga atacttccat caacatggat gtcgtgtttc
    21241 cgaccacgct ttagacatct tacgctataa agaagcggat gaaacagtat tagaagagat
    21301 ttttcaaaaa gcacgagcta acaaactatt atcttcagat gaaatcgatg cttatcttac
    21361 agaaacactg atacatttga cccacttcta tcattctcat aattggacga tgcagctgca
    21421 catccatgct tatcgtaact gtaatacaca aatgttggaa aaattagggc ccgataccgg
    21481 ctatgatgga atgaatgatc tttctttgac tattcctctg caaaaattat tgaaccgtgc
    21541 agaagaaacg gatcagttgc caaagacgat cctttattca ttgaatccga atgactatcc
    21601 tgcgatttta gctttgatgg gctgtttcca aaaagaacag aatggaaaac tgcagttagg
    21661 ttctggatgg tggtacaacg atactcgagc tggtatgcgc gatcaattga cacaatttgc
    21721 tgatggtagt gctttgggga attttgtcgg catgctgaca gattcaagaa gtttcctgtc
    21781 ctacactaga catgaatatt tccgtcgtgt tctttgtgaa tttttaggcg aaatggtcat
    21841 gcgaggagaa gcacctgaag atgaacaact tttaggaagt ctagttcaag cgattagtta
    21901 tacaaatgct aaaaattact ttggcttttt tgcctaattg attaatagta acagtaacga
    21961 atcaaaaaca ggatagtgaa ataacggaat ccgtttatcc acgttttaga gaagaagacg
    22021 gaccaaattt
//
LOCUS       DARBET010000040        21988 bp    DNA     linear   BCT 07-NOV-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4027
            SAMEA114569841-rid20112183.denovo.040, whole genome shotgun
            sequence.
ACCESSION   DARBET010000040 DARBET010000000
VERSION     DARBET010000040.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA114569841
            Sequence Read Archive: ERR12203666
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 21988)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 21988)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001972618.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001972618.1
            Genome Coverage       :: 132x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/07/2023 13:18:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,041
            CDSs (total)                      :: 2,975
            Genes (coding)                    :: 2,908
            CDSs (with protein)               :: 2,908
            Genes (RNA)                       :: 66
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 67
            CDSs (without protein)            :: 67
            Pseudo Genes (ambiguous residues) :: 0 of 67
            Pseudo Genes (frameshifted)       :: 26 of 67
            Pseudo Genes (incomplete)         :: 28 of 67
            Pseudo Genes (internal stop)      :: 20 of 67
            Pseudo Genes (multiple problems)  :: 6 of 67
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..21988
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA114569841-rid20112183.denovo.040"
                     /isolate="VRE4027"
                     /db_xref="taxon:1352"
                     /country="Denmark"
     gene            <1..395
                     /locus_tag="SCJ86_001694"
     CDS             <1..395
                     /locus_tag="SCJ86_001694"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347917.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEG1169235.1"
                     /translation="ISLKDESNTTVSDANILANIILDFIFEHHIPFKEGYEILPANQE
                     YYFYKCITKRVCCICGKTGADIDHFDKALGRRKRKEVDHAEYTFAALCRIHHTEKHKI
                     GVINFKNKYQIKGIKLNQKTIKKLNIGG"
     gene            398..1147
                     /locus_tag="SCJ86_001695"
     CDS             398..1147
                     /locus_tag="SCJ86_001695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343497.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HEG1169236.1"
                     /translation="MTEHRSYYAIIPANVRYDKRLKPNTKLLYGEITALCNERGFCWA
                     GNEYFADLYGVNKETISRWVSDLIKFGYLNREIIYKEGTNQIINRYLRINQYPIDEKR
                     NTPIDEKVKDNNTSINNTFNNTKEYIRELPPSKKSKAKPVRHKYGEYKNVLLSDEQME
                     KLKTEFPNDYQERIERLSEYCESSGKTYKNYLATIRSWARKEKSEPKNASSGYKRTGR
                     REKLPEWAIDQEAYLKKKALERANRQSKAPF"
     gene            1159..1428
                     /locus_tag="SCJ86_001696"
     CDS             1159..1428
                     /locus_tag="SCJ86_001696"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002352161.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEG1169237.1"
                     /translation="MKIDYLELINEIANYKKGEELDVLRDVYDQLEEAGIEGIKNDRS
                     SWSKLRYYFALYIDTTQLRNLAYTKLLFVDCVKGLQKHLNELEQV"
     gene            1433..1597
                     /locus_tag="SCJ86_001697"
     CDS             1433..1597
                     /locus_tag="SCJ86_001697"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEG1169238.1"
                     /translation="MDLKTFTAQIELMHQEALRQSVSYEDKWLNTFHGGRESALDQVL
                     KLLKGECRDG"
     gene            1590..1892
                     /locus_tag="SCJ86_001698"
     CDS             1590..1892
                     /locus_tag="SCJ86_001698"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEG1169239.1"
                     /translation="MDKKAAMQRIIELTYSEDWQNDKEAASEVMRLGRAMWADKSNKP
                     RPRKIAIWHGDKLLVIGTAEQLASLTGLHEKIVRKRARCGYTDAKKRTFRYVEESS"
     gene            1889..2050
                     /locus_tag="SCJ86_001699"
     CDS             1889..2050
                     /locus_tag="SCJ86_001699"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010752308.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antitoxin"
                     /protein_id="HEG1169240.1"
                     /translation="MTTEEVIQMRIRSLQREIDDLERTKAVMVNETARKAIDLHIENL
                     RREIHRLEE"
     gene            2047..2352
                     /locus_tag="SCJ86_001700"
     CDS             2047..2352
                     /locus_tag="SCJ86_001700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628584.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEG1169241.1"
                     /translation="MSVDKKAAMKRIAELTKSESWQEDKEIVAEVQKLGKSMWTEKPK
                     RRTPRKIAIWHDDRILVTGTAEQLSEITGLSKNIIWDRARSLWIDSKGRQFRYVEER"
     gene            2352..2708
                     /locus_tag="SCJ86_001701"
     CDS             2352..2708
                     /locus_tag="SCJ86_001701"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322164.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1140 family protein"
                     /protein_id="HEG1169242.1"
                     /translation="MDLITQYSDIILKKIMMKIQKDKKSKERAELVKLEMAETGAGVR
                     SSRHWKAAANIEFYYNEIQKGFDQMRELDRQTNWSKKLHQDRFKFVEKYKEILEEYLR
                     RTANDKKTRSIQHGFI"
     gene            2695..2913
                     /locus_tag="SCJ86_001702"
     CDS             2695..2913
                     /locus_tag="SCJ86_001702"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322165.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEG1169243.1"
                     /translation="MDLYDIESGATLSVESDHLIINFGGKRQIILWVVDDVLFPEIVH
                     DFEESKAVEFEIVKKVMELIEKYEEDSE"
     gene            2910..3329
                     /locus_tag="SCJ86_001703"
     CDS             2910..3329
                     /locus_tag="SCJ86_001703"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002352294.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YopX family protein"
                     /protein_id="HEG1169244.1"
                     /translation="MIPKFRAWDKRKNVMRDVAVLHFTKNGKTNFIEYWINPTELKSY
                     HVRNIDLMQSTGMKDKNGVDIFEGDIVLVSVRNGFDYLDNKVCIVKNSIDYSGLVCAT
                     VDEDLEYQIFNTELFEEYTYEVTGNIYENSELLEEQR"
     gene            3326..3883
                     /locus_tag="SCJ86_001704"
     CDS             3326..3883
                     /locus_tag="SCJ86_001704"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286545.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1642 domain-containing protein"
                     /protein_id="HEG1169245.1"
                     /translation="MNKQEAIKKLESIKAIGNDAIAACYNESINSGITLMKKIDEPQK
                     PVIPQLVAGWLEKSTDPFTKAEKIAYLIKSKDGDSYYFCDWFVRDGIVTQEQGEELLA
                     WATRQSYETLLSLYNGYEVEKEPLWAIKNADGNYLTKCALWGKDGVNYSFECNPSHRL
                     LFTDKATADAAALLVNGTVEEVVER"
     gene            3880..4176
                     /locus_tag="SCJ86_001705"
     CDS             3880..4176
                     /locus_tag="SCJ86_001705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349661.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEG1169246.1"
                     /translation="MKLKDGFYASSHGIGGLMLDMPTKNPKTRKKSKFKVGDMVRCEA
                     EEFVYPFRGYVKKILSNSAIIRIENTMKCDRSTAKHKHYLAVARLVDMEVIKSK"
     gene            4253..4666
                     /locus_tag="SCJ86_001706"
     CDS             4253..4666
                     /locus_tag="SCJ86_001706"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337268.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="autolysin"
                     /protein_id="HEG1169247.1"
                     /translation="MQLLREVDFKQTRCNARDVLKNFRRLERMAGRSLIDIKSPIITD
                     MPKAPKHGNKAEDAIIQMMDIEAERDAILAALMALSLISRQILYYSFCVPDSFSNYRI
                     SREVGYSERSIQRMKSEALIEFAEAYKHGRIIAYK"
     gene            5012..5386
                     /locus_tag="SCJ86_001707"
     CDS             5012..5386
                     /locus_tag="SCJ86_001707"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738779.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEG1169248.1"
                     /translation="MEDKMYLRQKVFTKKDIENQINGFIQKLENANSYLINKELNKAY
                     DQWKEVYDELKLIQNETKLVRTEKKNENSFFFDGYAILMLETVAKQNIKAPKKELSDN
                     IDNALAELRYYVMQIKDVRITR"
     gene            5423..5590
                     /locus_tag="SCJ86_001708"
     CDS             5423..5590
                     /locus_tag="SCJ86_001708"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEG1169249.1"
                     /translation="MYRPQYLEQKYEVITVQNGNGEIVRKYRRPIKSDTYKRKESNEV
                     IPLYGKRIAKH"
     gene            5616..5960
                     /locus_tag="SCJ86_001709"
     CDS             5616..5960
                     /locus_tag="SCJ86_001709"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286540.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HNH endonuclease"
                     /protein_id="HEG1169250.1"
                     /translation="MNEEFYRWLLQLIREDRLVKFYQSPKWRRLREKAMKRDHYECQE
                     CRRLGKYHRVENVHHIKEVKDRPDLALDLDNLICLCVEHHNEVHGRYLTALDKQEKKI
                     ESFANFDASERW"
     gene            5965..6246
                     /locus_tag="SCJ86_001710"
     CDS             5965..6246
                     /locus_tag="SCJ86_001710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340808.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEG1169251.1"
                     /translation="MIINDNGREYDTEKIEEYSSYTQGLIKRLIYVRYVGIRDLLSDN
                     CCSKYKVNQVREALNKDNNVERIKNVFGYSIEEINYYIDFAEAFIPMVR"
     gene            6349..6663
                     /locus_tag="SCJ86_001711"
     CDS             6349..6663
                     /locus_tag="SCJ86_001711"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286538.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="terminase"
                     /protein_id="HEG1169252.1"
                     /translation="MFKNELSQNRYREKLRRSLISQLESQKTNIEPFLDNVDRYISLW
                     ETAISLEEDISENGIRLENGKKNESVALLVSVNKQMGLMLDKLAITPELVGEANESIP
                     EL"
     gene            6641..8335
                     /locus_tag="SCJ86_001712"
     CDS             6641..8335
                     /locus_tag="SCJ86_001712"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286533.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="terminase large subunit"
                     /protein_id="HEG1169253.1"
                     /translation="MNQFLSYKHIENWFKAIEEGTIKVCKEQLLLKNYLEERVFTRED
                     IYFDKQMVEDSINIPAQYFPFELIPWEKFLQCFIYGVRWKKDKTLVFNRYLSLMGRGN
                     GKTGFASWNNFFLLTAKHGIKNYDIDIYANNESQAKTSFDDVFKVIKDHPDLDKKVFK
                     ATKEVIQNIATNSKLRYNTANARTKDGKRPGANRFDEIHENEDYSMINVATSGGGKIR
                     DYREFYDTTNGHVRGGPLDDIIEESKMILSGELGIDKDGAEFSSLFPFICRLDNDNEV
                     DDPDMWEKACPTINYNANLKRKMFQEYSQMQRNAGLRLTFMTKRMNRPMEDTRFAVAS
                     YDDVLHTKEKEFPEKMDEVIGTVDFADRRDFASVGLLGKYDKDVYFTQHTFIHESALR
                     LQNIKREVIDISIDQGKSQIVHGKNIEADYIVGWFLEMSNKYYIKKIAMDMYRAKILK
                     PALEEAGFTVEIVRSGSVTHGMLKDLVDDLFINQRLFFGDDAIMRWYCMNVYEEHISN
                     GNIRYEKIEPETRKTDGFFSFLHGLNFLDDIYDSAPVTVTNSSVENTGTGFTPLVF"
     gene            8355..9533
                     /locus_tag="SCJ86_001713"
     CDS             8355..9533
                     /locus_tag="SCJ86_001713"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage portal protein"
                     /protein_id="HEG1169254.1"
                     /translation="MGIFQKAVGYFTKKATVPLEEYFCKLQVDFVYRKFAIETCIDLI
                     ANAMSKAEFKSYEDGKNKKNDLYYRLNVAPNKKNNATEFRKKLIRRLIFYNEVLIVSP
                     SNNSSEIFIADSWDVTEYALKDDVFSQVQINNIVLDREFLESDVIYIKYADQQIRQLV
                     DAYYQAYGKLISSAMNVYKRSNARRYVLKGNLFRPQDNTTQDQINKMMTSQFKAFMEA
                     DNAGAVFQLQNEYTLEDFSGNFQSNSRDIKNLIDDIFEMTAAAFHVPKNLLKGDMSGL
                     SDQVDAFLMFEIIPIAELIQDAFNASLYEVEEYLSGNFVRVDTTMIKITSFKDLVDAI
                     DVGIRNGVFTINEGRERVGNDRSDKAMADEIFITKNNQQVSKGGEANDDNENISSSKE
                     "
     gene            9496..10182
                     /locus_tag="SCJ86_001714"
     CDS             9496..10182
                     /locus_tag="SCJ86_001714"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336342.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Clp protease ClpP"
                     /protein_id="HEG1169255.1"
                     /translation="MTTMKTFLAVKNEGAVPQIFIQGFIGSSWFFEGNTDKGIKNILD
                     SLGDQEEIEVVINSNGGDVFQGIAIGNLLKSNKAKVNVVINGLAASAASIIAMAGDTI
                     KIYNNAQLMIHRASTYGEGNVDDFRTIADQLESIDKSVKASYKTRFNGTDEALQELLE
                     KESFMDAETALSYGLVDEIIDAENSSGTEAKKEQSVEEILNDVKEKRAEKIAAFTAAL
                     NKTFGQGDVK"
     gene            10182..11342
                     /locus_tag="SCJ86_001715"
     CDS             10182..11342
                     /locus_tag="SCJ86_001715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336343.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage major capsid protein"
                     /protein_id="HEG1169256.1"
                     /translation="MTVKNLKGVTAASDQLMKAFKDGNEESFSAAMVSLSKEIQDKIL
                     EEATAKNQDQLVLMNRGQRVLTTQETKFYNEVVKNEGFAGVEELVPATVFERVFEDLE
                     QSHPLLQKITFVNTTGVTEWIVSRGVNPAWWGKLCEAVKKVLDNGFDVINMKQFKLSG
                     YIPVCKAMLDLGPVWLDRYVRTVLVESLRIALEQAIVDGTGKDMPVGMMRDMSKQTSG
                     EYAEKKAEPITALDAVTMGRLMARLSKFNIEGVNDPIYRNVNPSDVVLIVNPTDYWSK
                     VFPAKTVLTANGEYVQVLPVPVSDLQSTAVPEGKAVIGVASDYFMGVGSTLKIEASDE
                     YHFVEDERIYLAKQYANGQPKRNDSFIVLDISALGTTTTTTKPTTTTTTTQA"
     gene            11352..12227
                     /locus_tag="SCJ86_001716"
     CDS             11352..12227
                     /locus_tag="SCJ86_001716"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336344.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEG1169257.1"
                     /translation="MKYILCQPAINRFKWELEVCLTNLKKLGIKDIVLLFSRHDDQIP
                     IFFEKEYGVEVHVYDDLRDDKEYIPSIKPYLWWKYLEEDHSREDDRYFYIDSDVIFNK
                     RINLRKLPSKDDVWYCSDCCSYLSLDYIRSCENGENILKDMANIVNVTVESLETINTN
                     SGGAQWVINRPKANYWKKVYLDSNRLYRYLRGQKTNIQIWTAEMWARLWNMMYFNIGP
                     KVHEELDFCFATDPIEKVKEVKILHNAGVTTNDEDLFFKGRYVTSTPFDEDLSFVNKK
                     KCSYAYVKAIKAVVR"
     gene            12224..12535
                     /locus_tag="SCJ86_001717"
     CDS             12224..12535
                     /locus_tag="SCJ86_001717"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286523.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEG1169258.1"
                     /translation="MTPEQVTEELLIAVKDNIYVTWNEEDESIKKMIAKNAVYLQSKV
                     STTLSFSPESLEYGLLIERCRYDWNRALDEFEQNFASELLGFIQHYALQEYIAGDVNG
                     E"
     gene            12525..12878
                     /locus_tag="SCJ86_001718"
     CDS             12525..12878
                     /locus_tag="SCJ86_001718"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342092.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage head closure protein"
                     /protein_id="HEG1169259.1"
                     /translation="MANNRRLEETFNDGWLKILTQTTKRNELGKKIGVEDTEITSLKF
                     RNLSMRDSDITAMDAMGSKLTKKVKTPFHPIAKKFNKDQYFIVIDSMRYNVIYADYDN
                     FYIYFYLESVGEYGD"
     gene            12868..13269
                     /locus_tag="SCJ86_001719"
     CDS             12868..13269
                     /locus_tag="SCJ86_001719"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296598.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEG1169260.1"
                     /translation="MVIDNSKEKERLNKQISAIKTSLEEHFGLKLFQDSVGEDELPDD
                     FNYFILETGEIEMITEPKYSVGQNLYLTFYSENREDLTGDSLDIISLIQNRSIRFQRM
                     DPNHLKLENQDRYIDQLVFTFRRLLKSDCHG"
     gene            13262..13666
                     /locus_tag="SCJ86_001720"
     CDS             13262..13666
                     /locus_tag="SCJ86_001720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286516.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEG1169261.1"
                     /translation="MAKNSWELKINGHDELLVRMERYSSESERLINEALKSKGSAIAV
                     DRITEKIPVSEADLRRGHQHAKNSRPLKTQYINLGFIIRPTRKFEYLKYPDLGIGTSK
                     RNQPDEFMRRGLGLALDPITELLIRQFDKLNK"
     gene            13678..14286
                     /locus_tag="SCJ86_001721"
     CDS             13678..14286
                     /locus_tag="SCJ86_001721"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286512.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HEG1169262.1"
                     /translation="MAKTKTVVTTFDNVSIKRIAFNFKNAGNAIATNCNGQLDGETEM
                     QTVVKKCGATEVKSKSKPINMTVTITAHVPMEVYRRFNGLKQDGRIKPGIYSYGPDSV
                     GEDFSLAAEIVDDFEENRKLVGMLACTSNTGLTFSIENGADEVAALELETKVMQDEFG
                     KFYHEAIVAELEEDLTDQWMTNLSADVIKKTSVVTTTATQSQ"
     gene            14306..14668
                     /locus_tag="SCJ86_001722"
     CDS             14306..14668
                     /locus_tag="SCJ86_001722"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010737859.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEG1169263.1"
                     /translation="MNEDYSKIELNDGTILNLEPKLNIKKLLMINRDFNTDEFAKMTV
                     GKGSMDISVIQGAKAVYIAYRQANMTDYISFDEFIDKWDFDMATASYIYQLMMFKQAR
                     DAYQKEFEKANKEKKLQK"
     gene            14671..14853
                     /locus_tag="SCJ86_001723"
     CDS             14671..14853
                     /locus_tag="SCJ86_001723"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305377.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEG1169264.1"
                     /translation="MPKLLVETWVDVYSMLTDVFSMPSDLVLSDICLDDILQMAYNKS
                     AYEGWKNYAINQSQKN"
     gene            14870..18289
                     /locus_tag="SCJ86_001724"
     CDS             14870..18289
                     /locus_tag="SCJ86_001724"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342094.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail tape measure protein"
                     /protein_id="HEG1169265.1"
                     /translation="MAKKRTEAEVTFIANDDGLKSTLKEISAELTKNRAELKLEQAQL
                     QQTGSESDKLGSKLSSLEKQYELQSQKVEVTSQRLANAKKYYGENSTEVQKLERELIN
                     QQTAQQRLSNEIDKTSNTLAQAKGEIQTYESTMQQLDSEQKNVQASASLIESEYKKWQ
                     VTAGQSASESEKLAKAQEYVSQQSENAEKTIDILRRQLEATQSEFGATSTEAMQMEAK
                     LNDAEREFEELGQAAKNVDTTNLDDIGSKIDMNNLMEASDVLSDIGDKLTELGKQAVD
                     SANSVGSSQSKIQANFGLTKQEAEELTNVARDIYYKGFGESLDQSTDALILVKRNLGD
                     LNNQDLQNITEQAMVLENTMGADMDETLRGVNGLMVNFGLSAQDAMDLMVSGTQNGLD
                     KTHELGDNMAEYSQLWSQMGYSADETFGMLQNGLDAGAYNLDKVNDLVKEMGISLTDG
                     RFEQNMDMFSESTRKAFEEWKNGGGTQKDVINSMIQDFSNMDGQYDQLNKASTIWSAL
                     GEDNAMKVVQSLTDVNHTFDDVSGSAQKMNEDSTTPLQELNGKIAELKDSLAPIGNTI
                     IDALEPVIDFLGKMADAFNNLPQPVQDYAVAIGGLTAAFTLLMPIIVGFMALGGPTTL
                     IIGAVITAIAGVIAIIKNWGAITDWFKGIWSKFTDWLGGTWESIKEGASSVWDGVKET
                     WSGFVDWVQEIWQGVSDWFGELWSGLVEGASNIWQGVQETWQAFVDWVSNIWNGVKEV
                     WSIIWADIVGIVQIPWTLITSLIQAGINIIVGIFDVAGQLLGAAWQAVWTPISDFLKN
                     TWDTMTQWISIAWNGIVTTFHTIFDPVVAWWNGIWTSISTTASNIWNSISATASSIWN
                     SIKNTITSLVQAAATVIQNIWSTVSSWLGGIWNSISSTASNIWSSVTSSISNAINAAK
                     SAIQSVWNSISSWISGIWNGIKNTALNLWNGITSTISSKVNDGKNAISSGWSNLTGIV
                     SDIFNNVKSTIANIWEGIKKTVSAPIDWIRDKISSVFDNLNISIPHIPLPHFKLSGEF
                     NPLKGKIPTLGVDWYAKGSVFNSPNIIGVGESGPEAVLPLKRSVLQEIGDRILSSTSV
                     SSRTQAVQPVNNYEFNFTIDGNADEVTMKQTTQQIIDSITKVQNDNASAWR"
     gene            18340..19077
                     /locus_tag="SCJ86_001725"
     CDS             18340..19077
                     /locus_tag="SCJ86_001725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002351794.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HEG1169266.1"
                     /translation="MTDCIHSIIDGFPDYLHKLALAERPTIPSPKRQRVETSVLGRLG
                     GLVQDYSFEDMSFTLHYNYLEDVEDHQAFKQSFYIMRHWLNYAKKLEFSDDPNVYYVI
                     QTIDIGDAENDIVEWGEFDVNITAKPFARVQEDVPITVDKPQSFNLLNNSLEESFPKI
                     IITPSATSCQFILNDYVFSFEGLVAGTDVVIDSDLMLCYEEQSDGDILDRSNKMKTMQ
                     YPTLQVDINHFNCTGLSKIQIYRNGLR"
     gene            19087..21378
                     /locus_tag="SCJ86_001726"
     CDS             19087..21378
                     /locus_tag="SCJ86_001726"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002351381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HEG1169267.1"
                     /translation="MIDNLITIYDKNDANNLAEHLYDTQGLGALSDWLTATVSNKLNG
                     AEIFQGTYPISGTNADLIVEGRIIQCYVDENRAKQRLRIYYAKTSVIGNTIEVKAEPI
                     FNDIRKSVLNKYDSGTEKITATQAWQNAKALAKPAIPSQFSFSSLVDTLANVKIEKAN
                     FLEFFGGKEGSILDRFHGEFLKDNNTLHHEKRLGTDHKIKAIYTKNLTGLDLEIDAQS
                     VLVGVYPFISSSSEGEDEITLPEEVIFTDYVDDYPAGYVSFVDFKDKATDVATLREAA
                     KDWLKTNIDKQKPQVSGSIELVPLRHQRGYEKFVDLEKVSMGDGVDVYHPQLKVNMSA
                     RIVEYTFNVLTNSYDKLVVGNVKTNFLENTENNVSNLINDAIDQLKNGGEISDLINDI
                     VDHQTDMITGQNGGYVLLDPKEAPSRILIMDTPDKNTARNVLQINNAGIGFSKTGING
                     TYETAWTLDGGFNASFITAGEIVGITIRGTTLISDGTDYRTSIANGKMTWYSKKVNKD
                     IMELEARDYVSADAGIVSYTMKTGGGFMIRNPQGNLVFSTWDNGNNRPFLSFGAPNFR
                     YSNASYVTSGDGSSLSINGSAGNSWEFKVAGRTMKFTSDGMLTLPGCFFGSWEDGKLA
                     RFEQSTVQVYKDFTVRGTKNSTVPTEHYGQRLLNAYETPEYYFADYGEAVTGDDGKVR
                     VDIDPMFAETVNLSRYMTHVTPTELVLCAVTHEDIDHFIIETSKPNVLVRWNLVAHRL
                     GYEDIRLKEDTAYDSTVLDQKRF"
     gene            21402..>21988
                     /locus_tag="SCJ86_001727"
     CDS             21402..>21988
                     /locus_tag="SCJ86_001727"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347035.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEG1169268.1"
                     /translation="MASSLYNLALDFSKELNYTKAIMARQGDKGITVTVKPFLNGLQM
                     DTSGGTFTLKGTTPSNRYVDSVATSVTSEEVTFSLDGTFMSEAGYYKHCYVEYRKDNQ
                     ILTTQDIIFFSLGVSDISQGQADEYVSQLEELIQKYNETFDAFMAEIEGRVDSLNKQI
                     TDLTGQAKTLQDKLDALKEEISKLGNLQVMYSNSI"
BASE COUNT         7933 a         3245 c         4705 g         6105 t
ORIGIN      
        1 aaataagcct caaagacgaa tcgaatacaa ccgtgagtga tgccaatatc ttagcgaata
       61 tcatcctaga tttcatcttt gaacatcata ttcctttcaa agaaggatat gagattttac
      121 cagcgaatca agagtattac ttctacaaat gcattacaaa aagagtctgc tgcatctgtg
      181 gcaaaacagg agctgacatc gatcactttg acaaagcgct aggaagacga aagcgcaaag
      241 aagttgatca tgcagagtac acatttgcag cactctgcag aatccatcac acagagaagc
      301 acaaaatagg tgtgattaat ttcaaaaata aatatcaaat caaagggatc aaattaaacc
      361 agaaaacaat caaaaagtta aatattggag ggtaaaaatg acagaacatc gaagttatta
      421 tgcgattata ccagccaacg taaggtacga caaaagactt aaaccaaata ctaagttgtt
      481 atacggagag ataacggcct tgtgtaatga aagaggcttt tgttgggcag gcaatgagta
      541 ctttgcagat ttatatggtg tgaataaaga gaccatatcg cgatgggtaa gtgatttgat
      601 taagtttgga tacttgaatc gggaaatcat ttacaaagag ggtaccaatc aaataatcaa
      661 taggtaccta cgaattaatc aataccctat tgacgaaaaa cgcaataccc ctattgacga
      721 aaaagtcaaa gataataata catctattaa taatacattt aataatacaa aagaatatat
      781 aagagagtta ccgccttcga aaaaatcgaa ggctaagccc gtccgtcata aatacggaga
      841 gtataaaaat gttcttttgt cagatgagca gatggagaaa ctcaaaacag aattccctaa
      901 tgattaccaa gagcgaatcg aacgactgtc agagtattgt gaatcatctg gtaagactta
      961 taaaaactat ttggcaacta ttcgaagttg ggcaaggaaa gaaaaaagtg aacctaagaa
     1021 cgcaagcagt ggatacaagc gcacaggaag acgagagaag cttcctgaat gggcaatcga
     1081 ccaagaagcc tatctcaaga aaaaagcgct agaacgagct aatagacaat caaaagcacc
     1141 attctaagag gtggaaaaat gaagatcgat tatctagaac taattaatga aatagcgaat
     1201 tataaaaagg gcgaggaatt agacgtcctg agagacgtat atgatcaact cgaagaagct
     1261 ggaattgaag gaattaagaa tgatcgttcg agttggagta agctcagata ctatttcgca
     1321 ctttatatcg atacaacaca attaagaaat ttagcatata ccaaattact atttgttgat
     1381 tgtgttaaag gcttgcaaaa acatcttaat gaacttgagc aggtgtaatc agatggacct
     1441 aaagacattt acagcacaga ttgaactaat gcatcaagaa gctttaagac aaagtgtatc
     1501 gtacgaagac aagtggctca acacgttcca tggcggacgt gagagcgcac ttgatcaagt
     1561 actcaaatta ctgaaaggag aatgtcggga tggataagaa agcggcaatg cagcgaatta
     1621 tcgaattgac ttattcagaa gattggcaaa atgacaaaga agctgcttca gaagtgatga
     1681 ggcttggaag agcgatgtgg gcagacaaga gcaacaagcc aagaccacga aaaatcgcaa
     1741 tttggcacgg tgacaaactt ctagtgatag ggacagctga acagttagca agtctcacag
     1801 gcttgcacga gaaaatcgtg aggaaaagag caaggtgtgg ctacacagac gctaagaaga
     1861 gaacgtttag atacgtggag gaatcatcat gacaacagaa gaagtgattc aaatgcgtat
     1921 tcgaagcctt cagcgtgaga ttgacgatct ggaacgaaca aaggcagtga tggtcaatga
     1981 aacggcgaga aaggcaatcg atttgcacat agagaattta agaagggaaa tccatcgatt
     2041 ggaggaatga gcgtggataa gaaagcagca atgaaaagaa ttgctgaatt aaccaagtca
     2101 gaatcttggc aagaagacaa agaaatagtt gcagaagtcc aaaagctcgg taaatcaatg
     2161 tggactgaaa agcccaaacg gagaacgccg agaaaaattg caatctggca tgatgaccga
     2221 attttagtaa caggtactgc tgaacagtta tctgaaatta ctggattaag caaaaacatt
     2281 atctgggata gagctaggag cttatggatt gattcaaaag gacgacagtt taggtatgtg
     2341 gaggagagat aatggatctc attacacaat acagtgacat catcctcaag aaaatcatga
     2401 tgaagattca gaaagataaa aaatcaaaag aacgagctga attagttaag ttagaaatgg
     2461 ctgaaacagg agcaggagtg cgaagtagca ggcattggaa agcagcagca aacattgaat
     2521 tttattacaa cgaaattcaa aaagggttcg atcagatgcg tgagctggat cggcaaacaa
     2581 attggagcaa gaaacttcat caagatcgtt tcaaatttgt agagaagtat aaagaaatat
     2641 tagaagagta tttgaggagg acagcaaatg ataaaaaaac tcgttcaatt cagcatggat
     2701 ttatatgata tcgaatcagg agcaacacta tctgtggaat cggaccatct aatcataaat
     2761 tttggtggaa agcgccagat tattttgtgg gtagttgatg atgtactgtt tccagaaatt
     2821 gttcatgatt tcgaagaatc aaaagcggtt gagtttgaaa tagtgaaaaa agtaatggaa
     2881 ttgattgaaa aatacgagga ggacagcgaa tgataccgaa gtttagagcg tgggataaac
     2941 gaaagaacgt aatgagagat gtagccgtct tgcattttac taaaaacggc aaaacaaact
     3001 ttattgaata ttggataaat cctaccgaat tgaaatcata tcacgtgcga aacatcgacc
     3061 tcatgcaatc cacaggaatg aaagataaga atggtgtgga tatatttgaa ggggatatag
     3121 tattagtcag cgtgcgaaat ggcttcgatt acttagataa taaagtctgt attgtcaaaa
     3181 attcaataga ttattccgga ttagtttgtg ccactgttga tgaagactta gagtatcaaa
     3241 tttttaacac agagctgttt gaagaataca cgtacgaagt caccggaaat atatacgaga
     3301 atagcgagtt attggaggaa cagcgatgaa taaacaggaa gcaatcaaaa agttagaaag
     3361 tattaaagcg ataggaaatg atgcaatagc tgcttgctat aacgagagta taaattcagg
     3421 tattacgtta atgaaaaaaa tagacgaacc gcagaaacca gttattccac aactcgtggc
     3481 cggttggctt gagaaatcta cggacccttt tacaaaagct gaaaaaatag cgtatttaat
     3541 caaatctaaa gatggtgatt catattattt ctgtgattgg tttgtacgag atggcatagt
     3601 gacgcaagag caaggagaag aattactcgc ttgggcaaca agacaatcat atgaaacact
     3661 attgagccta tacaacggct acgaggttga gaaagagccg ttatgggcaa taaagaatgc
     3721 cgatggaaac tatcttacta aatgtgcttt atggggaaaa gatggagtaa attatagttt
     3781 tgaatgcaat ccatctcatc gattgctttt cactgataaa gcaacagcgg atgctgcagc
     3841 attgttggtg aatggaacag tggaagaggt ggtagaaaga tgaaactaaa agacggattt
     3901 tacgctagca gtcacggtat cggcggttta atgctagata tgccgacaaa gaaccctaaa
     3961 acacgcaaga aatcaaaatt caaagtcggt gacatggtcc gctgtgaagc agaagagttc
     4021 gtttatccgt tcagaggata cgttaaaaag atactgtcaa actcagcaat cattcgtatt
     4081 gaaaatacaa tgaagtgtga tcgttcgact gcaaaacaca agcattattt agctgtagcg
     4141 agattagttg acatggaagt aatcaagagc aaataaaaaa agccggatcg ctccgactga
     4201 ttcaataaat ccaacacatt tattatatca cataaaggag cggtttgact tgatgcaatt
     4261 gttacgagag gtagatttca aacagacaag atgtaatgcg agagatgtgc tgaagaactt
     4321 tcggcgtttg gagcggatgg caggtcgctc tttgatagat attaagtcgc cgattattac
     4381 ggatatgccg aaggcaccaa agcacggcaa taaggcagaa gacgcgatca ttcagatgat
     4441 ggatatagaa gcggagagag atgcgattct agcggctttg atggctctta gtctaattag
     4501 tcgccagata ctctactaca gcttctgtgt gccagatagc ttctcaaact acaggattag
     4561 ccgtgaagtg ggttattcag aaagaagtat acaacggatg aagtcggaag ctctaataga
     4621 gtttgcagaa gcatataaac acggaagaat aattgcttat aaataatttg gcggtttttt
     4681 ggcggaatga tggcggtttt tagctattta ccagtgatat tatggtagtg tcgaaagatt
     4741 agtgataggt ctaagacaaa ataaaatgta agggaggaaa tctccctcat cgtttaatta
     4801 agcttcgata gacagcagca aataaactaa aggatgtggg ggtcagctcc tacagttagt
     4861 tcatgtgttg ctgtctatta attgcaactc tttcggtttt attgagtatt tactcaactt
     4921 aaaaaaactg ttgtttgtag atgtaagctg ttcctatttg tacatagtaa aaaatgatat
     4981 aataaaaata ttacgaaagg ggtgagagtg catggaagac aaaatgtatt taagacaaaa
     5041 agttttcaca aaaaaagata ttgaaaatca aattaatgga tttatacaga aattagaaaa
     5101 tgcaaattct tatttgataa ataaggaact aaataaagct tatgaccagt ggaaagaagt
     5161 atatgatgag ttgaaattga ttcaaaatga aacaaaatta gtacgaacgg agaaaaagaa
     5221 tgaaaatagt ttcttttttg atggatatgc tatattgatg ttggaaactg tagcaaaaca
     5281 aaatataaaa gctcccaaaa aagaactatc tgacaatatt gataacgcat tagccgagtt
     5341 aaggtattac gttatgcaaa taaaagatgt taggatcact cgttgagtgg tctttttatt
     5401 ttgcacaaag gaggtaacaa caatgtatag accacaatac ttagaacaga agtatgaagt
     5461 aatcactgtg caaaatggta acggagagat agtacgaaag tatagaagac caataaagag
     5521 cgatacatat aaacgaaagg aaagcaatga agttattcca ttgtatggca aaagaatagc
     5581 taagcattaa ataagattgt gaaaggagac gggacatgaa cgaggaattc tatagatggc
     5641 tattacagtt gataagagaa gatcgtttgg ttaagttcta tcagtctcct aaatggcgca
     5701 ggcttagaga gaaagcgatg aaacgagatc actatgaatg ccaagagtgt agaagactag
     5761 gtaagtatca tagagtagag aacgttcatc atataaagga agtcaaggat agacctgact
     5821 tagctttaga tttagataat cttatttgtt tatgtgttga acatcataat gaagttcatg
     5881 gcagatatct tacagcattg gataaacaag agaagaagat agaaagcttt gctaacttcg
     5941 atgcaagtga aaggtggtaa gtgcatgatc atcaatgata atggcagaga gtatgataca
     6001 gaaaagattg aagagtattc atcttatact cagggattaa ttaaacgttt gatatacgtt
     6061 cgctatgtag gtattaggga tctgttatca gataactgtt gtagtaaata caaagtgaat
     6121 caagtaagag aagcgttgaa taaagataat aacgtcgaaa gaataaaaaa tgtttttgga
     6181 tatagtattg aagagattaa ttattacatt gacttcgctg aagctttcat tccgatggtg
     6241 agataacccc cccttaaaat aaatcgcaaa ttttttgggg gtgatgaaac ggagggggct
     6301 gtcaggaaaa gagatttttt cgaactttat catgaaagga gggctaaaat gtttaaaaac
     6361 gaattgtctc aaaatcgcta cagagaaaaa ttacgccgct ctttaataag ccaattggaa
     6421 agtcagaaaa caaatattga gccattctta gataatgttg atcgttatat cagtttatgg
     6481 gaaacggcga tatcactgga agaagatata tccgagaacg gcattagact ggagaatggt
     6541 aaaaagaatg aatcagtagc gttgcttgtt tctgtcaaca aacaaatggg attgatgttg
     6601 gataaacttg ccattactcc tgaattggta ggtgaagcaa atgaatcaat tcctgagtta
     6661 taagcatatt gaaaattggt tcaaagctat agaagaaggc actatcaagg tatgcaaaga
     6721 gcaattattg ctaaaaaatt atctagaaga aagagtcttt actagagaag atatttactt
     6781 cgataagcag atggtagagg attcaatcaa tataccagca caatactttc cattcgaatt
     6841 aattccgtgg gaaaaatttc tacaatgttt tatttatggt gttcgatgga aaaaagataa
     6901 aacactagtg ttcaatagat atctttcatt aatgggacgt ggtaatggta aaactggttt
     6961 tgcttcttgg aacaacttct ttctactaac cgctaaacac ggtattaaaa attatgatat
     7021 tgatatctat gccaataatg aaagccaagc aaagactagt tttgatgatg tatttaaagt
     7081 aattaaagat catcctgatt tagataaaaa agtatttaaa gctacgaagg aagttattca
     7141 aaatatcgct acaaacagca aacttcgtta taacacggca aatgctagaa caaaagatgg
     7201 gaagcgacca ggtgcaaacc gctttgatga aattcacgaa aatgaagatt attcaatgat
     7261 aaatgtggct acttctggtg gtggtaaaat tcgagattat agagaatttt atgatacaac
     7321 taatggtcat gttcgtggtg gtccgcttga tgacattata gaagaatcaa aaatgattct
     7381 ttctggagaa cttggaattg acaaggatgg agcagaattt tctagtttgt ttccatttat
     7441 ttgtcgcttg gataacgata atgaagttga tgatcccgac atgtgggaaa aagcttgtcc
     7501 aactattaat tacaatgcaa atttaaaacg gaaaatgttt caagaatact ctcaaatgca
     7561 acgtaatgct ggtttaagac ttacgttcat gaccaaacga atgaacagac ctatggaaga
     7621 tacacgattt gctgttgctt catatgatga tgttctgcat acgaaagaaa aagaatttcc
     7681 tgaaaaaatg gatgaagtga taggaacagt cgattttgct gatagacgag attttgccag
     7741 cgttgggttg ctaggaaaat acgataaaga tgtttatttt acacaacata cttttatcca
     7801 cgaatcagcc cttcgattac aaaacatcaa acgagaggtt atagatattt ctatagatca
     7861 aggaaaatca cagatcgttc atggaaaaaa tatagaagct gattatattg taggttggtt
     7921 tcttgaaatg agtaataaat attatattaa aaaaatcgct atggatatgt accgtgcaaa
     7981 aatattgaag cccgctttag aagaagcagg ttttactgtg gaaattgttc gaagcggatc
     8041 tgttacacat ggtatgttaa aagatctggt tgatgacctt tttattaatc aacgtttatt
     8101 ttttggtgac gatgcgatta tgcgttggta ttgcatgaat gtatatgaag agcatatttc
     8161 taatggaaat atacgctatg aaaaaataga acctgaaact agaaaaacgg atggcttttt
     8221 ttcattcctt catggtttga attttttaga tgatatttat gattctgctc ctgtaacagt
     8281 cacaaatagc tcagtagaaa atacaggaac tggatttact cctctagtat tctaacttga
     8341 aaggaggtga gaaagtgggg atttttcaaa aggcggtagg atacttcaca aaaaaagcaa
     8401 cggttccttt agaagaatac ttttgtaaat tgcaagttga ttttgtgtat cgaaaatttg
     8461 cgattgaaac ttgtattgat ttgattgcaa atgcgatgag taaagcggaa ttcaagtcat
     8521 atgaagatgg aaaaaataaa aagaatgatc tttactatag gctgaatgta gctcctaata
     8581 agaaaaataa tgcaacagaa tttagaaaaa aactgatcag gagattaata ttctacaatg
     8641 aagtattgat cgtttctccg tctaataatt ctagcgaaat atttattgcg gatagttggg
     8701 atgtcacaga atatgcattg aaagatgatg tgttttctca agtgcaaatt aacaacatag
     8761 tccttgatag agaatttcta gaaagtgatg ttatctatat aaaatacgca gatcaacaaa
     8821 ttaggcaact agtcgatgcg tattatcaag cgtatgggaa actcatttct agtgccatga
     8881 atgtttacaa gcgctctaac gctcgtagat acgtactgaa agggaattta ttccgaccgc
     8941 aagacaatac aacacaagat caaatcaata aaatgatgac atcacaattt aaggctttta
     9001 tggaagctga taatgcaggt gcggtatttc aattacaaaa tgagtacaca ttagaagatt
     9061 tcagcggaaa cttccaaagc aattcaagag atataaaaaa cttaatagac gacatctttg
     9121 agatgacagc agcagcgttt cacgttccga aaaacctact aaagggagac atgagtgggt
     9181 tatcggatca agtggacgct tttttaatgt tcgaaatcat accgattgct gaacttattc
     9241 aggatgcgtt taacgctagt ctctatgaag tagaagaata cttgtcaggg aattttgtac
     9301 gtgtggatac aactatgatc aagattacta gcttcaaaga tttggttgac gctattgatg
     9361 taggcattag aaatggagta tttacaatca acgaaggaag agaacgcgtt ggaaatgatc
     9421 gctctgataa ggcgatggca gatgaaatat ttataactaa aaacaatcaa caagtatcga
     9481 aaggaggtga ggcgaatgac gacaatgaaa acatttctag cagtaaagaa tgaaggcgca
     9541 gtaccgcaaa tttttattca gggatttatt ggttctagtt ggttctttga agggaatact
     9601 gacaagggaa tcaaaaatat tttggatagt ctaggtgatc aagaagaaat tgaagtagta
     9661 attaattcaa acggtggaga cgtatttcaa gggattgcta ttgggaactt acttaagtca
     9721 aataaagcaa aagttaacgt tgtgattaac ggcttagccg ctagtgctgc ttcaattatc
     9781 gcaatggctg gcgatactat aaaaatctac aacaatgcac aattgatgat tcaccgcgct
     9841 tccacatacg gagaaggaaa tgtcgatgac ttccgtacga ttgctgacca actggaatca
     9901 attgataaat cggtaaaggc ttcatataaa acacgattca atggcacaga tgaagcattg
     9961 caagaacttc ttgaaaaaga atcgtttatg gatgcagaaa cagctttgag ttatggattg
    10021 gtcgatgaaa ttatcgatgc agaaaatagc tcaggtactg aagctaaaaa agaacaaagc
    10081 gttgaagaaa ttttgaatga cgttaaagaa aaaagagcag aaaaaattgc tgcatttaca
    10141 gcagcattaa ataaaacatt tggacaagga gatgtaaaat aatgacagtt aaaaatttaa
    10201 aaggtgtaac agctgcaagc gaccaattga tgaaagcttt taaagatggt aacgaagaat
    10261 cttttagcgc agctatggta agcttatcta aggaaattca ggataaaatt ttagaagaag
    10321 caacagcaaa aaatcaagat caattagtat taatgaaccg tggtcagcgt gtattaacta
    10381 cacaagaaac aaaattctat aacgaagtag tgaaaaacga aggttttgca ggggtcgaag
    10441 aattagtgcc agctactgta tttgaacgtg tatttgaaga tttagaacaa tctcatccac
    10501 tattgcaaaa aattactttt gttaacacaa ctggtgtaac agaatggatt gtgtcacgtg
    10561 gagtcaatcc agcatggtgg ggtaaacttt gcgaagctgt taaaaaagtt ttagataatg
    10621 gctttgatgt aattaacatg aagcagttca agctatcagg ttatattcct gtatgtaagg
    10681 caatgcttga tttaggtcca gtatggttag atcgttatgt ccgtactgtt ttagtagaat
    10741 cattgagaat tgcattagaa caagcaattg ttgatggtac tggtaaagat atgccagtcg
    10801 gaatgatgcg tgacatgagc aaacaaacta gcggagaata tgctgaaaaa aaagcagaac
    10861 ctattacagc tttagatgct gtaactatgg gccgtttgat ggcgcgacta tcaaaattca
    10921 atatcgaagg tgtgaatgat ccgatttatc gtaatgtgaa tccttctgat gtggtcctaa
    10981 ttgtgaatcc aactgattac tggtctaaag tattcccagc taagactgta ctaactgcta
    11041 atggagaata cgtacaagta ttgccagtac cagtttcaga tttgcagtca acggctgtgc
    11101 cagaaggaaa agcagttatt ggggtagcct cagattactt catgggtgta ggatctacac
    11161 taaaaattga agcttcagat gaataccatt ttgttgaaga cgaacgcatt tatctagcta
    11221 aacaatatgc aaacggtcaa cctaaacgta acgatagttt cattgtatta gatattagcg
    11281 ctttgggaac tactactaca actacaaaac caacaaccac aacaactaca acacaagcgt
    11341 aggtgatcag aatgaagtat attctttgtc agccggcaat caatcggttt aaatgggagc
    11401 ttgaagtttg tttaactaat ctgaagaaac taggaatcaa agatatcgta ttgcttttca
    11461 gcagacacga tgatcagatt cctatttttt ttgagaagga atatggtgtt gaagttcatg
    11521 tgtacgatga tctgcgggac gacaaagagt atattccttc gattaaacca tatttatggt
    11581 ggaaatattt agaagaagat cattcgcgtg aggacgaccg atatttctat atcgattcgg
    11641 atgtcatttt caataaaaga attaatttgc gcaaattgcc ttctaaagat gatgtttggt
    11701 attgtagcga ctgctgtagt tatctaagtc ttgattatat tagaagctgt gaaaacggag
    11761 aaaatattct aaaagatatg gcaaacattg taaatgttac agtagaatct ttggaaacta
    11821 taaacactaa ttcaggaggc gcacagtggg ttattaaccg tcctaaagcg aattattgga
    11881 aaaaggttta tctggattct aatcggctat atcgctacct tagagggcaa aaaacaaata
    11941 tacaaatttg gacagccgag atgtgggcac ggctttggaa catgatgtat ttcaatattg
    12001 gtcctaaagt tcacgaggaa ttagactttt gttttgctac tgatccaata gaaaaagtta
    12061 aagaagtaaa aatcttacac aatgctggag taacaacaaa cgatgaagat ttatttttca
    12121 aagggagata cgtgacttct acgccttttg atgaagattt atcatttgta aacaagaaaa
    12181 aatgctctta cgcatatgtt aaagcaatta aggcggtggt tagatgacgc ctgaacaagt
    12241 gactgaagaa ttgctaatag ctgtgaagga taatatttac gttacctgga acgaagaaga
    12301 tgagtcaatt aaaaagatga tagctaaaaa tgctgtttat cttcaaagta aagtgagtac
    12361 aacactttct ttttctcctg aaagcttaga atacggattg ctaatcgaaa gatgtagata
    12421 cgactggaat cgtgctttag atgagtttga acaaaatttc gctagtgagt tattaggttt
    12481 cattcaacat tatgcgctac aagaatatat tgcaggtgat gtgaatggcg aataatcgta
    12541 gactcgaaga aacattcaac gatggttggt taaagatttt gacgcaaact accaaaagaa
    12601 atgaactagg aaaaaagatt ggtgtagaag atacagaaat cacttcttta aaatttagaa
    12661 atctttccat gagagatagt gatataacag ctatggatgc gatgggatcg aaattaacta
    12721 agaaagtaaa gactccattt catccaatcg ccaagaaatt taataaagat caatatttta
    12781 tcgtaatcga tagtatgcgt tacaacgtta tctatgccga ttacgataat ttttatatct
    12841 atttttatct tgaaagtgtg ggtgaatatg gtgattgata attctaaaga aaaagaacgt
    12901 ttaaataagc aaatttctgc tatcaaaact tccttagaag agcattttgg cctcaaactc
    12961 tttcaagact ccgtaggcga ggatgagcta cctgatgatt ttaattactt cattctcgaa
    13021 acaggagaaa tagaaatgat cactgagcca aaatatagcg tgggtcaaaa tctatatcta
    13081 actttctatt cagaaaatag agaagattta acaggagact cactagatat tatttcattg
    13141 attcaaaatc gttcgattcg ttttcagaga atggatccca atcatttaaa actagagaac
    13201 caagatcgct atatcgatca attggtattt acgtttagac gattattgaa gagtgattgt
    13261 catggctaaa aatagttggg agctaaaaat aaatggacat gatgaacttc ttgtgcggat
    13321 ggaacgctat tcaagcgaga gcgaacgact gattaacgaa gcattgaaat caaaaggttc
    13381 ggctattgca gtggatagga ttacagaaaa aattcctgtt tctgaagcag atttaagaag
    13441 agggcaccaa cacgcaaaaa atagtcgtcc acttaagact caatacatta atttgggttt
    13501 catcattaga cctacaagaa aatttgagta tttaaaatat cctgatttgg ggataggtac
    13561 ttctaaaaga aatcagccgg acgaatttat gagaagagga ttaggtcttg cacttgatcc
    13621 aattacagaa cttctgattc gtcaattcga taaattaaat aaataggggg aacaacaatg
    13681 gctaaaacaa aaactgtagt aacaacgttc gataacgtaa gtatcaaacg aattgctttt
    13741 aattttaaga acgcaggaaa tgcaatcgca acgaattgta atggacaatt agatggcgaa
    13801 acagaaatgc aaacggtggt taaaaaatgt ggagcgacag aagtaaaatc aaaatctaaa
    13861 ccaatcaata tgacggtaac aattactgca catgtaccga tggaagttta tcgacgtttc
    13921 aatgggttga aacaagatgg acgtattaaa ccaggcattt actcttacgg tcctgattcc
    13981 gtaggcgaag atttctcact tgctgcagag atcgtggatg acttcgaaga aaatagaaag
    14041 ttagttggta tgttagcatg cacttcgaat acaggattaa cattctctat tgaaaatggt
    14101 gcggatgaag tagctgcgtt agaactagaa acaaaagtta tgcaagatga atttggtaaa
    14161 ttttatcatg aagcaattgt tgcagaactt gaagaagact taacagatca atggatgacg
    14221 aatctatctg ctgatgtgat taaaaaaact tcagttgtga caactacggc cactcaatca
    14281 cagtaaaaaa aacggaggta gcgaaatgaa cgaagattac tcaaaaattg aactaaacga
    14341 tggaacaatt ttgaatttag aacctaaact gaatatcaag aaattattga tgatcaatag
    14401 agattttaac acagacgagt ttgcaaaaat gactgtggga aaaggatcca tggatatttc
    14461 tgttattcaa ggtgcaaagg ctgtgtatat tgcttaccgc caagcgaaca tgactgatta
    14521 tatttcattc gatgaattta tcgataaatg ggattttgat atggctactg ccagctatat
    14581 ttatcaattg atgatgttca aacaagcacg cgatgcttat caaaaagaat ttgaaaaagc
    14641 aaataaggaa aaaaagcttc aaaagtaaaa atgccaaagc tcttagttga aacgtgggtc
    14701 gatgtctatt cgatgttgac cgacgttttt tctatgcctt cagatttggt tttaagcgat
    14761 atctgtttag atgacatttt gcaaatggct tacaacaaga gtgcttatga aggatggaaa
    14821 aactatgcaa taaaccaatc tcagaaaaac taaagaaagg aggtaaaaaa tggctaaaaa
    14881 gagaacagaa gcagaagtaa ctttcatagc taacgatgac ggattgaaat ctacgttaaa
    14941 agaaatcagt gctgaattaa ctaaaaatag agcagaatta aaactagaac aagctcaatt
    15001 acaacagact ggttctgaat cagacaagtt aggaagtaaa ttatcttctt tagaaaagca
    15061 gtatgaatta caaagccaaa aagttgaagt aactagccaa cgtttagcca atgccaaaaa
    15121 atattatgga gaaaattcca ccgaagttca gaaacttgag agagaactga ttaaccaaca
    15181 aacagcgcaa caacgtttgt caaacgaaat tgataaaacg agtaatacac tagctcaagc
    15241 aaaaggcgaa atacagacgt acgagtctac aatgcaacag ttggatagtg aacaaaaaaa
    15301 tgttcaagct agtgcttctc tgattgaatc cgaatacaaa aaatggcaag taactgctgg
    15361 tcaatcagct tctgaatccg agaaattagc gaaagcccaa gaatatgttt ctcaacaatc
    15421 tgaaaatgca gagaaaacga tagatatcct gagacgacag ttagaagcta cacagtctga
    15481 gtttggcgct acatccacag aagcaatgca gatggaggcg aagcttaatg atgctgaacg
    15541 tgaatttgaa gagttaggac aagctgctaa aaatgtagat acaactaact tggacgatat
    15601 cggaagcaaa atagatatga ataatttaat ggaagcttct gacgttttaa gcgacattgg
    15661 cgataagctt acagaattag ggaaacaagc agtggactct gctaacagtg taggtagttc
    15721 ccagagtaaa atacaagcta attttggttt gactaaacaa gaggctgaag aattaacgaa
    15781 tgtagccaga gacatttatt ataaaggttt tggagaatcg ttagatcagt ccacagatgc
    15841 attgattttg gtaaagcgta atttaggcga tttaaataat caagatttac aaaatattac
    15901 ggaacaagct atggtcctag aaaacaccat gggcgctgat atggatgaaa cgttacgtgg
    15961 tgtaaatggc ttaatggtca acttcggatt gagcgctcaa gatgcaatgg atttaatggt
    16021 ttcgggtact caaaacggtt tagataaaac gcacgaatta ggcgacaata tggcagaata
    16081 tagccaatta tggagtcaaa tgggatattc agctgatgaa acgttcggaa tgcttcaaaa
    16141 tggtttagat gcgggtgctt ataaccttga taaagtcaat gacttagtta aggaaatggg
    16201 aatatcgtta acagatggtc gatttgagca aaacatggat atgtttagtg aaagtactag
    16261 aaaagctttt gaagagtgga aaaatggtgg aggaacacaa aaagacgtta ttaattccat
    16321 gattcaagat tttagcaata tggatggtca atacgaccaa ttaaataaag cttctacaat
    16381 ttggtctgca cttggcgaag ataacgcgat gaaagttgtc caatctttga ctgatgttaa
    16441 ccatacattt gatgatgtta gtggatctgc acaaaaaatg aatgaagatt ctactactcc
    16501 gttacaagaa ttaaacggaa aaatagctga attaaaggat tcattagctc ctataggcaa
    16561 cacaatcata gatgcactcg aaccagtaat tgattttcta ggaaagatgg ctgatgcgtt
    16621 taataatctt ccacaaccag tacaggatta tgccgtagcg attggcggat tgactgctgc
    16681 atttacttta ttaatgccaa taatagttgg cttcatggct ctaggtggtc ctactacatt
    16741 aataatagga gcagttatta ctgctattgc tggagttata gcaattataa aaaactgggg
    16801 cgcaattact gactggttta agggaatatg gagtaaattc actgattggt tgggtggtac
    16861 ttgggaaagc ataaaagaag gtgcctcatc agtttgggat ggagtcaaag aaacctggtc
    16921 tggatttgta gattgggttc aagaaatttg gcaaggagtt tctgattggt ttggagagtt
    16981 atggagcgga ttagttgaag gagcttccaa catctggcaa ggagtccaag agacttggca
    17041 agcattcgtt gattgggttt caaatatttg gaacggagtc aaagaagtat ggtcgattat
    17101 ttgggcagac attgtaggaa ttgttcaaat accatggaca ttaataacgt cattgattca
    17161 agctggtatt aatattatcg tgggtatctt tgatgtagct ggacagttat taggcgcagc
    17221 ttggcaagct gtttggacac ctatttctga tttccttaaa aatacttggg atactatgac
    17281 acaatggata agcatcgctt ggaatggaat tgtaactaca ttccatacta tatttgatcc
    17341 agtagtggca tggtggaatg gtatatggac atctattagt actacggctt caaatatttg
    17401 gaactcaatt agtgcaacag cttctagtat ttggaatagt atcaagaata caatcactag
    17461 cttggtacaa gcagctgcta cagtaattca aaatatttgg tcaactgtat ctagttggtt
    17521 aggtggaatt tggaattcaa tcagctctac agcatcaaat atctggagta gcgtgactag
    17581 tagtataagc aatgctataa acgcagctaa aagtgccatt caaagtgttt ggaatagtat
    17641 atcttcgtgg atcagcggaa tttggaacgg tatcaaaaac actgctttga atctttggaa
    17701 tggaattaca agcactatta gctctaaagt aaacgatgga aaaaatgcaa tttcaagcgg
    17761 ttggtccaat ctaacaggta ttgtttccga catattcaat aatgttaaaa gcacgatagc
    17821 taatatatgg gaaggtatca aaaagactgt tagcgctccg attgattgga ttagagataa
    17881 aatcagtagt gtctttgata atttgaatat ttctatacca catattccgt taccacattt
    17941 taaattgagc ggagaattca atccattgaa ggggaaaatc ccaacattgg gtgttgattg
    18001 gtatgcgaaa ggtagtgtgt ttaattctcc aaatattatc ggtgtaggtg aatcaggacc
    18061 tgaagcagtt ttacctttga aaagatctgt gctgcaagaa attggtgatc gtatcttgag
    18121 tagcacctca gtttcatcta ggacacaagc ggttcaacct gtgaacaatt acgaattcaa
    18181 tttcacaatt gatggtaacg cagatgaagt cactatgaag caaacaactc aacaaatcat
    18241 tgatagcatt acaaaagttc aaaatgataa tgcttcggca tggcgttaaa caggagagta
    18301 tttctcctgt ttttttagta ttaaaaagga tgtgaaaaaa tgactgattg tatacattct
    18361 ataatcgatg gatttcctga ttatttgcat aaattggctt tagctgaaag accaaccata
    18421 ccttctccaa aaagacagag agttgaaact tctgttttag gaaggttagg tggcttagtg
    18481 caagattact cgtttgaaga catgtcgttt acattgcact ataactattt agaggatgtg
    18541 gaagaccatc aagcgttcaa gcaatcgttt tatatcatgc gtcattggtt aaactatgca
    18601 aagaaattag aattctctga cgatcccaac gtctattacg ttatccagac tatcgatatt
    18661 ggggatgcag aaaacgatat tgttgaatgg ggagagttcg atgtaaatat tactgcgaaa
    18721 ccattcgcaa gagttcaaga agatgtacct ataaccgtag ataaaccaca gtcatttaac
    18781 ttgctgaata atagtttaga agaaagtttt ccaaagatta tcatcactcc ttcagctact
    18841 tcatgccagt tcatcttaaa tgattatgtg tttagtttcg aaggcttagt agcaggaact
    18901 gatgtagtca ttgatagtga tttgatgctt tgctacgaag agcaatcgga cggagatatt
    18961 ttagatcggt ccaacaaaat gaagaccatg caatatccga cattgcaagt ggatatcaat
    19021 cattttaatt gcactggttt gagcaaaata caaatttatc gtaacgggtt aaggtaggtg
    19081 aaatagatga tcgataattt aataactatt tacgataaaa atgacgcgaa taatttagct
    19141 gaacatttat atgatacgca aggtttaggc gctttgtcag actggttaac agctactgtt
    19201 agcaataaac taaacggagc cgagatattt cagggtactt atccaataag cggaactaat
    19261 gcagatttga ttgtagaagg acgtattatt cagtgttatg tagatgaaaa tcgagcaaaa
    19321 cagcgtctac ggatttatta tgcaaaaact tctgtaatag gcaatacgat agaagtaaaa
    19381 gctgaaccta ttttcaatga tataagaaaa tcggtgttga ataaatatga cagcggaaca
    19441 gaaaaaatca ctgctactca ggcatggcaa aacgcaaaag ctttagcgaa accagctatc
    19501 ccttcgcagt tttctttctc gtcattagta gatacgcttg ctaatgtgaa gatagaaaaa
    19561 gcgaattttt tagaattctt tggtggaaaa gagggatcta ttctagatcg atttcatggg
    19621 gaatttctta aagataataa cacattacat catgaaaaaa ggctaggtac ggatcataaa
    19681 atcaaagcga tttatactaa aaatttaact ggtcttgact tagagataga tgctcaaagc
    19741 gttttagttg gagtttatcc attcattagc agttcttcag aaggagaaga cgagatcact
    19801 ctaccagaag aagtcatttt cacggattac gtggatgatt atcctgctgg atatgtttct
    19861 tttgttgatt ttaaagacaa agcgactgat gtagccacat taagggaagc tgctaaagac
    19921 tggttgaaaa caaacataga taaacaaaaa ccacaagtga gtggttcgat tgaattagta
    19981 ccattgaggc atcaaagagg ctatgaaaaa tttgttgatc tagaaaaagt ttcgatgggt
    20041 gacggagtag atgtgtatca tccacagtta aaagtgaata tgtcagcgag aattgtggaa
    20101 tatacgttta atgttctaac caattcatac gataaattag ttgtaggaaa cgtcaaaaca
    20161 aacttcttag aaaacacaga gaataatgta agcaatttga ttaatgatgc cattgatcaa
    20221 ttgaaaaatg gtggcgaaat cagtgattta atcaatgata ttgtagatca tcaaactgat
    20281 atgattactg gtcaaaatgg tggttatgtt ttattagatc ctaaagaagc gcctagtcgt
    20341 attttgatta tggacacacc agataagaat accgcaagga atgttttaca aatcaacaat
    20401 gctggtattg gtttttctaa aactggcatt aatggaacat atgaaaccgc atggacatta
    20461 gatggcggat tcaatgcctc gtttattacg gctggtgaaa tagtaggaat tactattaga
    20521 ggtactacat taattagtga tggcactgat tatagaacaa gtattgctaa tggcaaaatg
    20581 acttggtact caaaaaaagt taacaaagat attatggagc tagaagcacg tgattatgta
    20641 agtgctgatg ccggtattgt atcatacacc atgaaaactg gtggtggttt catgattaga
    20701 aatccacagg gtaacttggt ttttagtacg tgggataatg gtaataacag accgtttcta
    20761 tcttttggtg cgcccaattt caggtatagc aatgctagtt atgtaacttc tggcgacggt
    20821 agttctttaa gcattaatgg tagtgcgggt aactcatggg aatttaaggt agctggtagg
    20881 actatgaaat ttactagtga tggtatgcta acgttgccag gttgtttttt tggttcatgg
    20941 gaagatggga aacttgctag gtttgaacaa tcaacggtac aagtatataa agattttact
    21001 gttagaggta ctaaaaactc aaccgtacca acagaacatt atggacaacg actattaaac
    21061 gcttatgaaa ctccagaata ttatttcgct gattatgggg aagccgttac aggtgatgat
    21121 ggtaaagttc gtgttgatat tgaccccatg tttgctgaga cagtaaatct aagtcgatat
    21181 atgacacatg tgacacctac agaactagtt ttgtgtgctg ttactcatga agatattgac
    21241 catttcatca ttgaaactag taagccaaac gtattagtta gatggaattt agtggcacac
    21301 cgtctagggt atgaagatat tagattaaaa gaggatacag catatgatag cacagtgctt
    21361 gaccaaaaac gtttttaaaa cgaagacaag gaggtatata aatggctagc agtttatata
    21421 atttggctct agatttcagc aaagaattaa actacaccaa agctattatg gctcgtcaag
    21481 gtgataaagg gattacagtg actgttaaac catttttaaa tggcttgcag atggatacga
    21541 gtggcggaac atttacttta aaaggaacaa cgccatctaa ccgttacgta gatagtgtgg
    21601 caactagtgt aactagtgaa gaagtcacgt tttctcttga tggcacattt atgagtgaag
    21661 caggatatta taaacactgc tacgtagaat atagaaaaga caatcaaatt ttaacaacgc
    21721 aagatatcat ttttttctca ctaggagtgt ctgacatttc gcaaggccaa gccgatgaat
    21781 acgtttcgca attggaagag ttgattcaaa agtataacga aacttttgat gcttttatgg
    21841 ctgaaatcga aggtagagtg gacagcttaa ataaacagat tactgattta actggtcaag
    21901 ctaaaacact acaagacaag ttagacgctc tgaaagaaga aatttctaaa ttaggtaact
    21961 tgcaggtgat gtacagtaac agcatcga
//
LOCUS       DARBET010000041        21912 bp    DNA     linear   BCT 07-NOV-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4027
            SAMEA114569841-rid20112183.denovo.041, whole genome shotgun
            sequence.
ACCESSION   DARBET010000041 DARBET010000000
VERSION     DARBET010000041.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA114569841
            Sequence Read Archive: ERR12203666
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 21912)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 21912)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001972618.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001972618.1
            Genome Coverage       :: 132x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/07/2023 13:18:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,041
            CDSs (total)                      :: 2,975
            Genes (coding)                    :: 2,908
            CDSs (with protein)               :: 2,908
            Genes (RNA)                       :: 66
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 67
            CDSs (without protein)            :: 67
            Pseudo Genes (ambiguous residues) :: 0 of 67
            Pseudo Genes (frameshifted)       :: 26 of 67
            Pseudo Genes (incomplete)         :: 28 of 67
            Pseudo Genes (internal stop)      :: 20 of 67
            Pseudo Genes (multiple problems)  :: 6 of 67
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..21912
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA114569841-rid20112183.denovo.041"
                     /isolate="VRE4027"
                     /db_xref="taxon:1352"
                     /country="Denmark"
     gene            complement(459..1088)
                     /gene="udk"
                     /locus_tag="SCJ86_001728"
     CDS             complement(459..1088)
                     /gene="udk"
                     /locus_tag="SCJ86_001728"
                     /EC_number="2.7.1.48"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289751.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uridine kinase"
                     /protein_id="HEG1169269.1"
                     /translation="MTKSKPIIIGVTGGSGSGKTSVSRAIFNNFPDHSIMMLEQDSYY
                     KDQSHLSFEERLNTNYDHPFAFDNDLLIQHVGDLLNYKAIEKPVYDYVAHTRSQATII
                     QEPKEVIILEGILILEDERLRDLMDIKVYVDTDDDIRIIRRIKRDMEERGRTLDSVIE
                     QYLTVVKPMYHQFIEPTKRYADIIVPEGGENHVAIDLITTKVASFLNHK"
     gene            complement(1172..1867)
                     /locus_tag="SCJ86_001729"
     CDS             complement(1172..1867)
                     /locus_tag="SCJ86_001729"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317068.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YjjG family noncanonical pyrimidine
                     nucleotidase"
                     /protein_id="HEG1169270.1"
                     /translation="MKYKTLLFDVDDTLLDFQLTEANALSALFAEQGITLTPEIETSY
                     KALNHHLWQEFEKGRLSREEIIGSRFGLLFQQFGKTVDSDQMEKRYRHYLNQGHDLVP
                     GSLEILKKVHPHADLYIVTNGVSHTQHRRLTDAGMLDYFKDIFVSEAVGAQKPMKEFF
                     DHVFSHIPNFDKKQTVIIGDSLTSDIKGGNMAGIDTIWFNRNKLPEIEEIQPTYRIDS
                     LEELYSLLEIEKD"
     gene            complement(1880..2596)
                     /locus_tag="SCJ86_001730"
     CDS             complement(1880..2596)
                     /locus_tag="SCJ86_001730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pseudouridine synthase"
                     /protein_id="HEG1169271.1"
                     /translation="MRLDKLIEEKLETTRKEMKRLFALKQVMIDGTVEFRQNRNVDSL
                     LHQIEVAGEHLETNESYYLLNKPEKAVTAVSDPEKTTVIDLIASADRFQPLYPVGRLD
                     RDTTGLLLITSNGQLGYEMLLPEKKVSKTYQVVVNERVTPADVTAFENGIVFADGYQC
                     KPADLKILKADKHQSTVLLTIQEGKFHQVKKMFLAQGKKVTALKRLSMGPLELDEQLG
                     VGEYRSLTLAEMKKLSIYFK"
     gene            2926..3534
                     /locus_tag="SCJ86_001731"
     CDS             2926..3534
                     /locus_tag="SCJ86_001731"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331169.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L25/general stress protein
                     Ctc"
                     /protein_id="HEG1169272.1"
                     /translation="MSVSLEVSKREVRPRSLRNKLRHEGKVPAVVYGYKVESTPISFD
                     GLTLSKLLREHGANTVITMDIDGQKVNTLVHKIQTNTFTNHFEHAEFLSVNMSEETEV
                     ETEVVLVGESVGAKSGGVLAQNLYTVVVSATPDKLPERIEVDVTNLELGDSITVADLP
                     KNDDYKFVTDSEEQIAAVVAQSEEEESTGEGGEPEVIGSTEE"
     gene            3859..4182
                     /locus_tag="SCJ86_001732"
     CDS             3859..4182
                     /locus_tag="SCJ86_001732"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010720345.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF960 domain-containing protein"
                     /protein_id="HEG1169273.1"
                     /translation="MFESFDSSRNRYASVGVVSSLPDELIDSIWFIIDLDLKGVIPLE
                     NILTFDLIENRGKVTMHFSQAGSNVEMDIDLPFAYSNQFPAQVYAYDDGTRETILLPS
                     EIRQY"
     gene            4364..5734
                     /locus_tag="SCJ86_001733"
     CDS             4364..5734
                     /locus_tag="SCJ86_001733"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349866.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MATE family efflux transporter"
                     /protein_id="HEG1169274.1"
                     /translation="MRDLTKGSPAKLILMFTVPLLIGNVFQQFYNMIDMIIVGQTLGK
                     NALAAVGATGSLTFLIIGFAQGLTAGLAIITAQRYGAKDYRGLKKSFAASVVISLVVT
                     VILTVLSLLFIRPMLQLMQTPPEILDQAQTFISIILLGIFASVSFNLLSNVIRALGDS
                     RTPLFFLIIAVIINVVLDLIFIIYFGMGVEGAAIATVIAQVSSSVLCLVYIKKKMPLL
                     QLRKKDFKFEKEEFAVHLNAALPMAFQSSIIAVGAIVLQAALNSLGTDVVAAQAAAGR
                     IDQFANQPMMSFGIAMATFTAQNYGAKEYGRILQGVRQTLLMSVGFSLLAGAIVIFFG
                     HFFVQLFVSPSETRVFELAQTYFNINGSLYWILAVLFILRYTLQGLGQSKIPTIAGMM
                     ELLMRSFAAIVLTGMWGYPGAAAASPLAWIGSVAVLLYSYMRSMKQLKHMGEEEHFTL
                     EHAEAR"
     gene            complement(5864..7165)
                     /locus_tag="SCJ86_001734"
     CDS             complement(5864..7165)
                     /locus_tag="SCJ86_001734"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HlyC/CorC family transporter"
                     /protein_id="HEG1169275.1"
                     /translation="MVINFIILFLMIAITSFFVASEFALVKIRRSRLEQLEKENVKNA
                     KLALHVTHHLDNYLSASQLGITLTGLIIGWVGEGSVAALLEPVIGKLPFSSAISSTIS
                     VALGFILVTYIDVVIGELLPKSYSIVNTEKVVLFVVKPLHYFYKVMFPFIWVLNHSAA
                     GLGKLLGVRLVSEGEETLSQEELTLVALNSYEKGELTKEEYYYLENVFEFDDTLAKDI
                     QVDRTSMQVFEASETVKEAIKASIEQGHTRYPVILESKDNVLGYVTLPDLIKQSYKDD
                     QLTVEQLIEEPIVTTETIPIKKLLTIMRKKGKHIAILKDEYGGTSGLVTIEDILEEIV
                     GEIRDETDLDEALIAEQSDGSYIISGKLTLDDFQRYFHVEIPEFEETNFTTLAGFASS
                     RYKEIKAGTIIEIASFRFTVLEYQHAHIDYFKVESTERKTE"
     gene            complement(7519..9477)
                     /locus_tag="SCJ86_001735"
     CDS             complement(7519..9477)
                     /locus_tag="SCJ86_001735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628634.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="KUP/HAK/KT family potassium transporter"
                     /protein_id="HEG1169276.1"
                     /translation="MTGLLVAMGVVYGDIGTSPLYVMKSIVEDNGGLSGINPDFVIGS
                     VSLIFWTLTLLTTIKYVVIALNADNHGEGGIFSLYTLVRKGGKYLIIPAMIGGAALLA
                     DGVLTPAVTVTTAIEGLRGIPQFFDRFGNNQNVIVIITLVIIFVLFMVQRFGTDIVGK
                     AFGPIMFAWFTFLGVMGLMNFSQDWTVIRALNPYYAIQLLFSADNKLGLFILGNIFLA
                     TTGAEALYSDLGHVGKRNIRLSWPYVKICLVINYFGQAAWLLNVYQNPEAQQIKNLNP
                     FFQMMPQSWIVIGVAFATVAAVIASQALITGSFTLVSEAIKLKLLPRLKIMYPGNSIG
                     QMYIPAVNLILWIACSLVVVTFRTSHHMEAAYGLSITVTMLMTTALLYFYLIQNGHSR
                     WLAYLVTFFFGAIEVVFFISSVVKFFRGGFVAVLIGLIILVVMFIWEQGNIIRESSTE
                     DVSLRDYIPQLRELRDDTSLPIYQTNVVFLVPDMAEDKVGRQFIYSILDKRPKRARVY
                     WFVHVEVTDEPYTKEYQVDMMDTDFIVQVNLFLGFRVQQEINVYVRQVIHDLMKQGRL
                     PKQPQRYSLTPGREVGDFQFVLIDEVVSNVTTLGKWQRQIMQAKLAIKKIATSPETWF
                     GLEYSEVKHETVPLIIGNTRKTWLKERL"
     gene            complement(9654..9920)
                     /locus_tag="SCJ86_001736"
     CDS             complement(9654..9920)
                     /locus_tag="SCJ86_001736"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376985.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GlsB/YeaQ/YmgE family stress response membrane
                     protein"
                     /protein_id="HEG1169277.1"
                     /translation="MLNFLWSLFIGGILGLIAGAILGKEVPGGVSGNIIIGFLGSWLG
                     EFLLGPLGPELGGFYLIPALLGAIFCLALYSFVARRIRERPKKE"
     gene            complement(10430..12556)
                     /gene="pnp"
                     /locus_tag="SCJ86_001737"
     CDS             complement(10430..12556)
                     /gene="pnp"
                     /locus_tag="SCJ86_001737"
                     /EC_number="2.7.7.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317067.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polyribonucleotide nucleotidyltransferase"
                     /protein_id="HEG1169278.1"
                     /translation="MSEKQVFKTTWGGRPLQIEVGQLAKQANGAVLVRYGDTVVLSAA
                     VASKEAKDTDFFPLTINYEEKMYAAGKIPGGFIKREGRPSTEATLTARLIDRPIRPMF
                     AEGFRNEVQVTNIVMSVETDCSPAMAAMLGSSLALSISDIPFDGPIAGVEVGRVNGEY
                     VLNPTVEQAEQTDIELTVAGTKQAINMVESGAKEVSEEDMLGALLFGFDAIKELVAFQ
                     EEIVQAVGKEKMEVTLLQVDEVLKKEIFDASYATMKAAVMTEEKLAREDNIEQVKIDI
                     REAYAEKFAGHEDEDHLLKEVKQITEDLEKDVVRELITIDKIRPDGRKLDEIRPLSSE
                     VSLLPRVHGSGLFTRGQTQALSACTLAPLGEHQIIDGLGVEVSKRFIHHYNFPQFSVG
                     STGRAGSPGRREIGHGALGERALAQVIPSEEEFPYTIRLVAEVLESNGSSSQASICAG
                     TLALMDAGVPIKAPVAGIAMGLVSDGENYTILTDIQGLEDHLGDMDFKVAGTKDGITA
                     LQMDIKIQGITEQILTEALTQAKQARMEILEELTSTIAAPREELSQYAPKIEMIQIEP
                     AKIKDVIGKGGDTINGIIDETGVKIDIDQDGKVSIASADAEMIKKAIKIIEDLTKEVK
                     VGEVYLGKVVRIEKFGAFVNLIKGKDGLVHISQLANDRVNKVEDVVKLGDEILVKVTE
                     IDKQGRVNLSRKAMLNEDGSTKNEEK"
     gene            complement(12744..13013)
                     /gene="rpsO"
                     /locus_tag="SCJ86_001738"
     CDS             complement(12744..13013)
                     /gene="rpsO"
                     /locus_tag="SCJ86_001738"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002354983.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S15"
                     /protein_id="HEG1169279.1"
                     /translation="MAISKERKNEIINEYARHEGDTGSPEVQIAVLTEEINHLNEHAR
                     VHKKDHHSYRGLMKKVGHRRNLLAYLRKTDVQRYRELIKRLGLRR"
     gene            13264..13827
                     /locus_tag="SCJ86_001739"
     CDS             13264..13827
                     /locus_tag="SCJ86_001739"
                     /EC_number="3.5.1.88"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623744.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide deformylase"
                     /protein_id="HEG1169280.1"
                     /translation="MITMDDIIREGNPTLREVAKEVSLPLSEEDISLGKEMLEFLKNS
                     QDPIKAEELHLRGGVGLAAPQLDISKRIIAVHVPSPDPEADGPSLSTVMYNPKILSHS
                     VQDACLGEGEGCLSVDREVPGYVVRHAKITVSYYDMNGEKHKIRLKNYESIVVQHEID
                     HINGVMFYDHINDQNPFALKEGVLVIE"
     gene            complement(14080..14601)
                     /locus_tag="SCJ86_001740"
     CDS             complement(14080..14601)
                     /locus_tag="SCJ86_001740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341084.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription repressor NadR"
                     /protein_id="HEG1169281.1"
                     /translation="MIEGETRRKQIMQTLANTEKPVSASKFAKTFGVSRQIIVGDIAL
                     LRAAGKSIVATARGYILETEETNTGHISKIAVQHGKDQTEEELRLIVENGGEIIDVIV
                     EHPLYGELAGTLHIKTPQDIDSFMRRYQKSNATLLSELTGGIHLHTIRYPEKHMLKNI
                     KQKLAEAGILYEG"
     gene            complement(14604..15446)
                     /locus_tag="SCJ86_001741"
     CDS             complement(14604..15446)
                     /locus_tag="SCJ86_001741"
                     /EC_number="5.4.99.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chorismate mutase"
                     /protein_id="HEG1169282.1"
                     /translation="MTEFKTEKEKMIAGELYFANDPELVADRMFARSQSQIINQAESA
                     ELRSQLLKETFGRTGKKIYMEPVINFDYGYNIFVGENFYANFNCTFLDVSTIEIGDNC
                     MFAPNVQLYTATHPLHPVKRNSGLEYAKPIKIGNNVWLGGGVIVTPGVTLGDNVVVGA
                     GSVVTKSFPDNVVIAGNPARIIKTVEEELTEESLETLRHKIDMIDRELVALLEKRMDT
                     VTKIGQVKKEENQAVYDAKREQQVLDKVVSLLKNKGYKETITDTYVDLMKHSREYQNK
                     MKEE"
     gene            complement(15439..16164)
                     /locus_tag="SCJ86_001742"
     CDS             complement(15439..16164)
                     /locus_tag="SCJ86_001742"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327537.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent protein deacylase"
                     /protein_id="HEG1169283.1"
                     /translation="MKQLTVDEAAKEILAYQQITFLTGAGVSTPSGVPDYRSLSGIYH
                     GMEQPEYLLSHQAMVNEPDKFYQFVKKIYHPEAKPNIIHLEIAHLAKEKNVWVVSQNI
                     DGLHAKAKSPQLVNFHGSLYQCHCRKCQQTVDWKEYLHSDKHQACGGQIRPDIVLYGE
                     GFQDGVMEQAAYAVSQAELIVIVGTSFQVHPFCDLVQFRQPSAKILVINQTPVYLAEP
                     YTFIQEDGAVVFKKIQELGADYD"
     gene            16340..16636
                     /locus_tag="SCJ86_001743"
     CDS             16340..16636
                     /locus_tag="SCJ86_001743"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339483.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEG1169284.1"
                     /translation="MLPFFIYWGVILACIAWLALSVYFSVFYLVRKENGNLWAFALFN
                     VIAAIVLAITLAVYRTWGWGITQYSSLIYLILAIYGVVVLLQAILGREPKKAAA"
     gene            17204..18796
                     /locus_tag="SCJ86_001744"
     CDS             17204..18796
                     /locus_tag="SCJ86_001744"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327538.1"
                     /note="The N-terminal region of this protein, as described
                     by TIGR01726, is a three transmembrane segment that
                     identifies a subfamily of ABC transporter permease
                     subunits, which specificities that include histidine,
                     arginine, glutamine, glutamate, L-cystine (sic), the
                     opines (in Agrobacterium) octopine and nopaline, etc;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HEG1169285.1"
                     /translation="MKKNMVSVAVLFAFVFSLFSVGSLANADEKTPSDEFRVGMEAGY
                     APFNWSQQTDENNALPIQGQNSYAGGYDVQIAKKVADGLGKKLVIVQTKWDGLAPALQ
                     SGKIDAIIAGMSPTAERRKEIDFTDPYYESQLVVVVQKDSKYAKAKSLADLSGAKITA
                     QLNTFHYTVIDQIPDVQKQQAMDNFSAMRTALASGMIDGYVSERPEGVTATSVNKDLE
                     MLEFSGADGFQTNPEDVQISVGMRKGDPDIQKVNQILSGISNDERTKIMDQAVKDQPA
                     ATDSEEKPGIISDFTTIWKQYGNMFLRGAGLTLFIALIGTVVGTTLGLLIGVFRSVPE
                     SGNALTRFFQKIGNALLSIYVEVFRGTPMMVQAMVIFYGLALAFGISLNRTIAALFIV
                     SLNTGAYMTEIVRGGIFAVDEGQFEAAQAIGMTHGQTMRKVVIPQVLRNILPATGNEF
                     VINIKDTAVLSVIGVADLFFQGNSASGANFQFFQTFTIVGIIYLVMTFTITRILRAVE
                     KKIDGPSAYTKLEEIDNSNLQE"
     gene            18809..19549
                     /locus_tag="SCJ86_001745"
     CDS             18809..19549
                     /locus_tag="SCJ86_001745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter ATP-binding protein"
                     /protein_id="HEG1169286.1"
                     /translation="MNEKIIEIKHLRKSFGENEVLKDISVTVNKGEVVTIIGSSGSGK
                     STFLRCINLLEKPTGGEIIYNGENVLAPKYNLPKYRTNLGMVFQSFNLFNNMNVLENC
                     MSGQTTVLKRDKETAKKVAIENLEKVGMDRYIEAKPAQLSGGQKQRVAIARALSMNPD
                     VLLFDEPTSALDPEMVGEVLNTIKDLAHTGLTMLIVTHEMEFAKDVSDRVIFMDKGVI
                     AEEGTPEDIFVHPKEERTKEFLSRILNA"
     gene            19717..21066
                     /locus_tag="SCJ86_001746"
     CDS             19717..21066
                     /locus_tag="SCJ86_001746"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294242.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HEG1169287.1"
                     /translation="MNEEQELQRGLKNRHVQLISIGGAIGTGLFLGSGKSIQLAGPSI
                     LLAYLITGCICFFIMRALGELLLSNTNNHSFLDFVAEYLGKKAAFITGWTYWFCWVSI
                     AMADLTAIGMYVRYWAPGVPQWLPELIALGLLLCLNMVAVSLFGELEFWFALIKVVAI
                     IAFILVGAYMILTHYTTDIGTASITNLWSHGGFFPTGAKGFILAFQMVTFAFAGIELV
                     GLVAGETENPEKVLPEAINNIPIRIILFYLGSLFVIMAIYPWNSLNPDNSPFVEVFSE
                     IGITIAASLINLVVLSAAASACNSAIYSTGRMLRSLAQEGSAPKKFKKLTTHHVPGNA
                     LTFSTIVIFISVILNYVMPSEVFTLVSSIATTCFLFIWSMLVYTHMKYRKSILGKKAH
                     SFKMPLYPFSNYLVFLYMAFVCVVLFLGKDTRIALLLTPVWFLLLLLIYHMKYERKK"
     gene            complement(21194..21784)
                     /locus_tag="SCJ86_001747"
     CDS             complement(21194..21784)
                     /locus_tag="SCJ86_001747"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376975.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1211 domain-containing protein"
                     /protein_id="HEG1169288.1"
                     /translation="MTKSRLEAFTDGVVAIVLRVIVLEIKIPNTSSFHSLWVIRNTLL
                     AYTISFIFVAVIWVNHHRIFQMAERINYRVVWSNIFLLFWLTLCPSVTSWVGRNPEAF
                     WPEMAYVLVYTMWSFSFGILMRQIMKANKPDSHVVRVLSKDRRSLISMMINLCLIAGV
                     FFVPLIGLFGRFFVSGIWIFSYKKADAYYQRLFPGK"
BASE COUNT         6295 a         4462 c         4158 g         6997 t
ORIGIN      
        1 gggggtgcga caaaagagct tcttcaaaaa aataagtcga aatctcacac aaattcgaga
       61 aataattttc gtaagtttct tcttatttat tgcatctgac cacttctgtt acaacctctc
      121 gttaaacggt gttcaaaagc ttgcatctac gataataagc ccaacccaac gaaaaacgtg
      181 aggagctgtt ttatttggat gctcttatcg cttgatgcaa agacagcttt tttacaacct
      241 ctacttcacg gtgctcaatc agctgacctt cggtattagc tcaaaaaacg gtaaaatata
      301 aacagctatt ttccgtttct tctttttaat acttaggtca agatgctgtt ttcacaacct
      361 cttgttataa ataaaaaaac agttggctgg tgactgttca aaaagcagaa tccttgcttt
      421 tcaaacggtc gcagtccgtt caactgtttc gctgcgcact atttatggtt taagaaacta
      481 gctacttttg ttgtaatcaa atcaatagca acgtggtttt ctccgccttc tggaacaatg
      541 atatccgcat agcgcttcgt tggttcgata aattgatgat acatcggttt aacaactgtc
      601 aaatattgtt cgatcacgga gtccaatgtg cgtccccgtt cttccatatc ccgtttgata
      661 cgacgaatga tacgaatgtc atcatctgta tctacgtata ctttgatatc catcaagtca
      721 cgcagacgtt cgtcttctaa aatcaggatt ccttctagaa tgatcacttc ttttggttcc
      781 tgaatgatcg ttgcctgact tcttgtatgc gctacatagt catacacggg tttttcaatg
      841 gctttatagt tcagtaagtc gcctacatgc tgaatcagca gatcattatc aaaggcaaat
      901 gggtgatcgt aatttgtgtt caatcgttct tcaaaactca aatggctctg atccttgtaa
      961 taagaatctt gttcaagcat catgatggaa tggtccggga agttgttgaa aatcgcacgg
     1021 ctgacacttg tctttccgct tccggatcca cccgtcacac caatgataat tggcttgctt
     1081 tttgtcattg actaaaacct ctttcgtatc cgttttttca ttctaatcta ttatagcgaa
     1141 aaaacacgaa aatgctatat ttttctgaaa attaatcctt ttcaatttcc aataatgaat
     1201 aaagttcctc taatgaatcg attcgataag taggctggat ttcttcgatt tctggtaatt
     1261 tatttctatt gaaccaaatc gtatcgatcc ctgccatatt tcctcctttg atatcagaag
     1321 tcaaagaatc accaatgatc acagtttgct ttttatcaaa attaggaatg tgggaaaaga
     1381 catgatcaaa aaattctttc atcggctttt gcgctccgac agcttctgag acaaagatat
     1441 ccttgaagta atcaagcatt cctgcatctg ttaatcgccg atgttgcgtg tgcgacacac
     1501 cgttcgtcac aatataaaga tcagcatgcg gatggacctt tttcaggatt tctagacttc
     1561 ctggaacaag atcatgacct tggttcaagt agtggcggta gcgtttttcc atttgatcac
     1621 tatctactgt tttcccaaat tgctggaaaa gtaacccaaa acgactgcct attatttctt
     1681 ctcgtgatag tctgcctttt tcaaactcct gccataaatg atgattcaaa gccttatagc
     1741 tggtttcgat ttctggtgtc aatgtgattc cttgttctgc aaacaaagca cttaaagcat
     1801 tggcttcagt taattgaaaa tctaacaatg tatcatctac atcaaacaat aaagttttat
     1861 atttcatctt tttgacctcc tatttaaaat aaatacttaa ctttttcatc tctgctaatg
     1921 tcaacgatcg atactcgcct actcctagct gttcatctaa ttccaatggt cccatgctta
     1981 gccgtttcaa tgcggtcact ttctttcctt gggctaagaa catctttttg acttgatgga
     2041 attttccttc ttggatcgtc aataaaaccg tactttggtg cttgtctgct ttgagtattt
     2101 ttaaatcagc cggcttacat tgatacccat cagcgaatac gattccattt tcaaatgctg
     2161 ttacgtctgc cggtgtcacc ctttcgttca caactacttg ataagtcttt gaaacctttt
     2221 tttctggaag tagcatttca tatcccaatt gtccattcga agtgatcagc aatagtcctg
     2281 ttgtatctct atctagtctt cccactggat ataatggttg gaaacgatcg gcacttgcaa
     2341 tcagatcgat tactgtcgtc ttttctggat ctgatacagc tgtaacagct ttttcaggtt
     2401 tattgagaag ataataggac tcatttgttt ctaagtgttc acctgctact tcgatttgat
     2461 gcagtaaact atctacattt cgattttgtc gaaattcgac tgtgccatct atcatcactt
     2521 gcttcaaagc aaacagccgc ttcatttcct tgcgtgtcgt ttctaatttt tcttctatta
     2581 atttatctaa acgcatgctg tttcctttct tattactact aattaattat accttgaata
     2641 ttcttgcttg caaatacatc atcatagtta ttccttgact taattaacag ttcacgttac
     2701 ggttaaatag taaagtgaat tggatttctc atacacagcg taatgacctg gtggagaaaa
     2761 gttgaatcct gactttctaa ggggagatgc gcgcatctct ccttttgcgc ttttttaagc
     2821 gccctgcctt cgatgatgtg cacgagatta gcctttagaa ttcacttaaa taaaatctgt
     2881 ccgttaccaa agcagacagt cacattttaa ggaggaattt tggttatgtc agtatcatta
     2941 gaagtaagta aaagagaagt acgtccccgt tcattaagaa acaaacttcg tcacgaaggg
     3001 aaagttccag ctgttgttta tgggtataaa gtagaaagta cccctatctc attcgatggt
     3061 ttgacactaa gcaaattgtt gcgtgaacat ggagcgaaca cagtcatcac gatggacatc
     3121 gatggacaaa aagtcaatac attggttcac aagatccaaa cgaacacttt taccaatcat
     3181 ttcgaacatg cagaatttct atctgtaaac atgtcggaag aaacagaagt cgaaacagaa
     3241 gtcgttcttg ttggcgaatc agtgggtgct aaatctggtg gagtgttagc tcaaaacttg
     3301 tatacagtag tcgtatctgc tacaccagac aaattgcctg aacgaattga agttgacgtg
     3361 acaaacttgg aattaggtga ttctattaca gtagccgatc ttccaaaaaa cgacgattac
     3421 aaattcgtga ccgacagtga agagcaaatc gctgctgtag tcgctcaaag tgaagaagaa
     3481 gaaagcacag gtgaaggtgg cgaaccagaa gtgatcggtt caacagaaga ataatactga
     3541 aacaatcagg tggtgttaga agcggcgggt cagcagcttt taacacgcct tttttatttc
     3601 taatcgttta ttttcatatg gggcttccac ttttgtaaat ggctttacaa acgaaaaatc
     3661 ctaatttacc aagcttttcc tcgcatactt ttatcgtacc tttataaaaa tttatacttt
     3721 gaaaaaaagt attcaattat tgttgcattt ttcctgattc tttattagaa tgaaccctcg
     3781 ttgaagtaag tatttctagt ttatcctagg attaattata aaaataaaac attcgttgat
     3841 tcgaaggagg ttaaacagat gtttgaaagt tttgatagca gccgcaaccg ttacgcttca
     3901 gttggtgtcg tatcaagttt gccagatgaa ttgattgaca gtatatggtt tattattgac
     3961 ctagatctta agggagtcat tccattagaa aatatattaa cctttgattt gatcgaaaat
     4021 cgtggaaaag tgacgatgca tttttctcaa gcaggaagca atgtggaaat ggatattgat
     4081 ttgccgtttg cctattcgaa ccaatttcct gctcaagtat acgcctatga tgacggtacg
     4141 agagaaacga tccttttacc aagtgagatt agacaatatt aaggtgatag ataaacgtgg
     4201 atgtccctta ttttacagac caggccaagt tgttgattct ttgactcaat tcttggcttc
     4261 tttttatgga gtttcataaa ttatcattga tttctaattg gtattctcag gataaacgct
     4321 atatactact aactaaattt taaagatata aggagtttaa aagatgcgcg atttaacaaa
     4381 aggaagcccc gctaaactga ttttgatgtt cactgttcct ttactgatcg gaaatgtttt
     4441 tcagcagttc tacaatatga tcgatatgat catcgtgggt cagacgctag gtaaaaatgc
     4501 gctggcagcg gttggtgcaa ctggaagcct gacatttttg atcattggct ttgcccaagg
     4561 tttaacagct ggtttagcca ttattaccgc acaacgttac ggtgcaaaag attatcgggg
     4621 tctgaagaaa agttttgctg ctagtgttgt cattagtctt gtcgtcactg tgattttaac
     4681 tgtcctaagt ctcctgttta ttcgtccgat gcttcaattg atgcaaactc ctcctgaaat
     4741 acttgatcaa gcacaaacat ttatttcaat tattttatta gggatctttg catcagtcag
     4801 tttcaatttg ctttctaatg tcattcgcgc acttggtgac agccgcacac cactattctt
     4861 tctgatcatt gcggtcatca tcaacgtcgt acttgatttg atctttatta tctattttgg
     4921 catgggtgtg gaaggtgcag ctattgctac agtaatcgca caagtttctt ccagcgtact
     4981 gtgtctagtg tatatcaaga aaaaaatgcc tttgttacag ttacgcaaaa aagatttcaa
     5041 atttgaaaaa gaagagtttg ctgtacacct aaatgctgct ttgccaatgg cgtttcaatc
     5101 ttcgatcatc gctgttggtg ctatcgtgtt acaagcagca ctaaatagtc tagggacaga
     5161 tgtcgtagct gctcaagccg ctgctggtcg tatcgaccaa tttgcaaatc agccgatgat
     5221 gtcatttggt attgcaatgg caactttcac tgcacaaaac tatggtgcga aagaatatgg
     5281 ccgaatcttg caaggggtac gtcaaacttt actgatgagt gtcggattta gtctcttagc
     5341 aggtgctatc gtcattttct tcggtcattt ctttgttcaa ttgtttgtta gcccatcaga
     5401 aacacgtgta tttgaattag cacaaaccta cttcaatatc aatgggtctc tttactggat
     5461 cttagcagtc ctgttcattt tacgctacac acttcaaggg cttggacaaa gcaagatccc
     5521 tacgatcgcc ggtatgatgg aattgcttat gcgttctttt gcagctatcg ttcttacagg
     5581 aatgtgggga tacccaggag cagcagcagc ttctccttta gcttggatcg gttctgttgc
     5641 cgtgttgctt tactcttata tgcgttcgat gaaacaattg aaacacatgg gtgaagagga
     5701 acatttcaca ttagaacatg cagaagcaag atagccttga ttaaaagaaa tcgttgcggc
     5761 taaaaatgaa aacttttatt gaaaatatct tcttcgttta ccgaaaacag gttttttaga
     5821 taacaaaaag gctgtgacaa aaaatgtcac agcctttttt cttttattct gtttttcttt
     5881 ccgtggactc tactttgaaa taatcgatat gtgcatgctg gtattctaat accgtgaaac
     5941 ggaatgatgc aatctcaatg attgttcctg cttttatttc tttatatcgg ctagaagcaa
     6001 agcccgcaag tgtagtaaaa ttcgtttctt caaactctgg aatctctaca tgaaaataac
     6061 gctggaaatc atctaatgtt agtttgccgg agatgatata agagccatct gactgttccg
     6121 ctatcaatgc ttcgtccaaa tctgtctcat ctcggatttc cccgacgatt tcttctaaaa
     6181 tatcttcaat cgtcactaag ccgcttgtcc ctccgtattc atcttttaag atagcaatat
     6241 gcttaccctt ttttcgcatg atcgtcaata atttttttat agggatcgtt tcagttgtta
     6301 cgatcggctc ttcaatcaac tgttctactg tcagctggtc atctttataa gattgcttga
     6361 tcaaatctgg caaggtgaca taacctaaaa cattgtcttt tgattctaag ataaccggat
     6421 agcgagtatg tccttgttca atcgaagctt ttatcgcttc tttgacggtt tctgaagctt
     6481 cgaatacttg catggacgta cggtctactt gtatatcttt tgccaaggta tcatcaaatt
     6541 caaacacgtt ttccaaataa taatattctt ctttggtcaa ctctcctttt tcataagaat
     6601 tcaatgcgac taatgtcagc tcttcttgag acaatgtctc ttctccttca gatacgagac
     6661 gcacacctaa tagcttccct agccctgcag ctgaatgatt caatacccaa ataaaaggaa
     6721 acattacttt ataaaaataa tgaagtggtt taactacgaa caagacgacc ttctccgtat
     6781 tgacgatact atagctctta ggaagcagtt cgccaataac cacatcgata tacgtaacta
     6841 aaataaatcc cagcgccaca gagattgtac tgctaatagc cgaactaaaa ggtagtttac
     6901 cgatcactgg ttctagtaaa gccgcgacag atccttcacc gacccacccg atgatcaaac
     6961 cggttagcgt aattcctaat tgactagctg ataaatagtt atctaaatgg tgtgtaacat
     7021 gaagagctag cttggcgttc tttacatttt ccttttctag ttgttccaat cttgatcgtc
     7081 tgattttcac taaagcaaat tcacttgcta caaaaaatga agtaattgca atcatcaaaa
     7141 ataaaataat gaaattgata accatattct cccgcctttc tctttttcta ttgtaccctt
     7201 tttctacaaa aagattgaaa ataatgcgta ttcatattta ggaataaaaa aggagatgcg
     7261 gcatttttca gccacatctc cttatcactt caaaatcatt gcgttacttg ttgctaaaca
     7321 gctttttcat gacctctgat aaaaacggta ttcaaaagct tgcatctacg gcattaagcc
     7381 caaacagatg aaaaacatga gaagctgttt ttatctgctc gttcttactg cttgttgcag
     7441 tacagctttt tcatgacctc tgataaacgg tgttcaagta gctgacattc gacattagtt
     7501 caggtcaaag cgctactttc acaaccgctc tttcaaccat gttttccgag tatttccaat
     7561 gataagcgga acagtttcat gttttacttc gctgtactct agcccaaacc atgtttctgg
     7621 cgaggtagcg atttttttga ttgccagttt tgcctgcatg atttgacgtt gccattttcc
     7681 tagagttgtc acattggaaa ctacttcatc aatcaggaca aattggaaat cgccaacttc
     7741 tcgccctggt gtcagagagt atctttgtgg ctgttttggc aaacggcctt gtttcatcaa
     7801 atcatggatg acttggcgaa catatacgtt gatttcctgt tggaccctga aacctaagaa
     7861 cagattcact tggacaataa aatcagtgtc catcatatct acttgatatt ctttcgtata
     7921 tggctcatcc gtcacttcca catgaacaaa ccaatagact cgtgctcttt taggccgttt
     7981 atctaaaatc gaatagatga actgtcggcc aactttatct tcagccatat caggaaccaa
     8041 gaagaccaca tttgtctgat agattggtaa agaagtatca tcacgtaatt ctctaagctg
     8101 cgggatataa tctctcaaag acacatcctc ggttgacgat tcgcgaataa tgttcccttg
     8161 ttcccaaata aacatcacga ctaagatgat caaaccaatc agaacggcta caaaaccgcc
     8221 tcggaagaat ttgacaacac ttgagataaa gaaaacgact tctatcgcac cgaagaaaaa
     8281 ggtaactaaa tatgcaagcc aacgagaatg cccattttgg ataagataaa agtaaagcag
     8341 tgctgttgtc atcaacatcg tcacagtgat cgatagaccg taagcagctt ccatatgatg
     8401 agacgttcgg aatgtgacaa ccactagaga acaggcgatc cataaaatca gattgactgc
     8461 tgggatatac atttgcccga tactgttccc aggatacatg atctttaacc gaggcagcag
     8521 tttcaacttg attgcttctg aaactagcgt aaacgaacct gtaatcaatg cttgcgaagc
     8581 aatgactgca gcgactgttg caaaagcaac gccgatcacg atccaacttt gaggcatcat
     8641 ttggaagaat ggattcaggt tcttgatttg ctgtgcttca gggttttgat aaacattcaa
     8701 caaccacgca gcctgcccaa aataattgat aaccaaacaa attttgacgt aaggccagct
     8761 cagacgaata tttcgtttgc cgacatgtcc aagatcggaa taaagcgcct ctgcacctgt
     8821 cgtagccagg aaaatatttc caagaatgaa caagcctaat ttgttatctg cactaaaaag
     8881 cagctggata gcataataag gattcagtgc acggataact gtccagtctt gactgaagtt
     8941 cattaatccc attacgccta aaaaagtaaa ccaagcgaac atgattggtc cgaatgcttt
     9001 tccgacaata tctgtaccaa agcgttggac catgaataaa acaaaaataa taactaaagt
     9061 gataatgacg atcacgttct ggttattacc gaaccggtca aaaaactgcg gaattcctcg
     9121 caagccttca atcgcagtcg ttaccgtcac agcaggggtc aataccccat ctgctagtaa
     9181 ggcagctcct ccgatcattg caggaatgat caaatatttc ccaccttttc ggacaagtgt
     9241 gtagagtgag aaaattccac cttcaccatg gttatctgca ttcaatgcaa tcacgacata
     9301 tttgattgtt gtcagtagag tcaatgtcca aaaaatcaat gagactgatc cgataacaaa
     9361 atcaggattg attcctgaca atccgccatt atcttcaaca atcgatttca taacgtaaag
     9421 cggacttgtc ccgatgtctc cgtagacgac tcccatcgct acaagcaggc cagtcattga
     9481 tatctttttg gtattgtgcg ttgttgccac gtttttccct catttcttgt tatatggata
     9541 tcctttaggt aagcttgaat gatagatgat attcttatcc atgacacccg ctttcctaaa
     9601 ttgcttctaa ttatccaagt aacacactga agtgtcaact tatttgaata aaattattct
     9661 ttctttggac gttctctgat acgtcttgcc acaaatgaat aaagagcaag gcaaaaaatt
     9721 gcacccaata atgctggtat caaataaaaa ccgccgagtt ccggtcccag tggtcctagt
     9781 aaaaattcgc cgagccaact acctaaaaaa ccgataatga tatttccaga tacgcctccc
     9841 gggacttcct ttcctaaaat tgcgccagct atcaaaccta atataccgcc gataaataac
     9901 gaccataaaa aattcagcat ctcctttccc tcctttatcc atatttctca cttttatttt
     9961 acgctttatt cagaaggact ccaaaaatat gcgccaagaa actccagaaa aaaagaagtt
    10021 gtaacaaaag tgttcagctt atttccgtag aagctgcttc tggaacaccg tgaatcagag
    10081 gttgtgaaat cagcggtttg acctgagtat tagagaaaca aacggaaaaa tagttcctca
    10141 tattttgccg ttttttgagc taatatcgag ggacagctga ttgaacatcg tttattggag
    10201 aggttgtaaa aaagctgttc tgcatcaagt aataagaaca gccaaataaa aacagctcct
    10261 aatgttttta acttggctgg acttattacc gcagatgcaa gcttttgaac accgtttatt
    10321 cgaaaaaaaa ggactgtgac acggcttttg tcacagtccc cttttttcga ataattattg
    10381 tccccctgtc accttcacga tcacaagtac attattccta tgaattaaat tatttttctt
    10441 catttttcgt tgaaccatct tcgttcaaca ttgcttttct tgacagattt acgcgtcctt
    10501 gtttatcgat ttcagttact ttcacaagga tctcatcacc tagtttaaca acatcttcga
    10561 ctttattgac acgatcgttt gctaattggg aaatatggac aagtccatct tttcctttga
    10621 tcaagttgac gaatgcaccg aatttttcga tacggacaac tttccctaga taaacttcac
    10681 caactttgac ttctttggtc aaatcttcga tgatcttgat agcttttttg atcatttctg
    10741 catcagctga agcaatactt acttttccat cttgatcgat atcgattttc acaccagttt
    10801 catcgatgat tccgttgatc gtatcgccgc ctttaccgat aacatctttg attttagcag
    10861 gttcgatctg gatcatttcg atttttggtg catattggct taattcttca cgtggagcag
    10921 caatcgttga agtcaattct tctaagattt ccatacgtgc ttgttttgct tgagttaatg
    10981 cttcagttag gatttgttct gtgattcctt gaatcttgat atccatctgt aaagcagtga
    11041 taccatcttt tgtaccggca actttaaagt ccatatcgcc aagatgatct tccagccctt
    11101 ggatatcagt taggatcgta tagttttcac catcagaaac aagtcccatc gcaataccgg
    11161 cgactggcgc tttgattggt acacctgcat ccatcaatgc taatgtgcca gcacagatac
    11221 ttgcttggga agaagaacca tttgattcca atacttctgc tactaaacgg atcgtataag
    11281 gaaattcttc ttctgaaggg attacttgtg ctaacgcacg ttcacctaat gcgccatgtc
    11341 cgatttcccg acgccctgga gagcctgcac gtcctgtaga accaacagaa aattgaggga
    11401 aattgtagtg gtggatgaat cgtttgctga cttccacccc taaaccatca atgatttggt
    11461 gctctcctag aggtgcgagt gtacaagctg ataaagcttg tgtttgtcct cgtgtgaaca
    11521 aacctgatcc atgtacacgt ggcaataagc tgacctctga tgacaatgga cggatttcat
    11581 ccaatttacg cccgtcagga cggatcttat cgatggtgat caattcacgt acgacatctt
    11641 tttcaagatc ttctgtgatt tgtttaactt ctttcaacaa gtgatcttca tcttcatgac
    11701 ctgcaaattt ctcagcataa gcttcgcgga tatcgatctt cacctgctcg atattatctt
    11761 cgcgagcaag tttttcttca gtcattactg ctgctttcat tgtagcgtaa gaagcatcaa
    11821 agatttcttt tttcaatact tcatcaactt gcagtaaagt cacttccatt ttttctttcc
    11881 ctactgcttg aacgatttct tcttggaaag caactaactc tttgatagcg tcaaaaccaa
    11941 acagcaatgc accaagcatg tcttcttctg aaacttcttt cgcgccgctt tcaaccatat
    12001 tgatggcttg tttcgttccg gcaacagtca attcgatatc tgtttgttct gcttgttcta
    12061 ctgttggatt caagacgtac tcgccattta cacgaccaac ttctacgcct gcgattgggc
    12121 cgtcaaatgg gatatctgag attgatagtg ctaaagaaga acctagcatt gctgccatag
    12181 ctggcgagca gtctgtttct acactcatca cgatgttcgt tacttggact tcattacgga
    12241 agccttctgc aaacatcggg cgaattggac ggtcgatcaa acgggcagtc aatgttgctt
    12301 ccgtacttgg acggccttct cgtttgatga aacctccagg gattttccct gctgcataca
    12361 ttttttcttc ataattgatc gttaatggga aaaagtctgt atcttttgct tctttagagg
    12421 ccacagctgc acttaagact actgtatctc cataacggac taaaacggct ccgtttgctt
    12481 gttttgctag ctgaccgact tcgatttgta agggacggcc gccccatgtc gttttgaata
    12541 cttgtttttc tgacataact tctccttttc cttctcgtga aagcaagacg ctactaaaca
    12601 aatgaaaact gccgaaagcc gtcttttgag cagctttcat ttggttagta ggagtccatc
    12661 tagcttgatc acggaatttt acttaattga tatacgagta aaaaaagtga gattcatgtg
    12721 aatcccactc tttttgacta aaattaacga cgcaagccta aacgtttgat caattcgcgg
    12781 taacgttgaa cgtcagtttt acgtaagtaa gctaacaagt tacggcggtg accaactttt
    12841 ttcattaggc cgcggtatga atgatggtct tttttgtgca cgcgtgcatg ttcattcaag
    12901 tggttgattt cttcagttaa tacagcgatt tgtacttctg gagaaccagt atccccttca
    12961 tggcgagcgt attcgttgat gatttcattc ttgcgttctt ttgaaattgc catttctttc
    13021 acctctttcg attattcccc tgcgactgag taaaacgttg gtgattcgct taaccaagtg
    13081 gcaggtcatg tgcctagagc acacagctat actttacctg aaaccgggtt gaaaatcaag
    13141 tagttaatca ttaagaaaag gtagttttca aacaatctta ctactacttt tccatttttg
    13201 tggtaaacta taatgcgaaa atagacttga aacacagtct tttaagaaag gggtacatat
    13261 tccatgatta caatggatga tatcattcgt gaaggaaatc cgaccttacg cgaagttgca
    13321 aaagaagttt ctcttccatt aagcgaagaa gatatctctt taggaaaaga gatgcttgag
    13381 ttcttaaaaa acagccaaga tccaatcaaa gctgaagaac tacacttacg aggcggtgtt
    13441 ggcttagctg ctcctcaatt agatatttct aaaagaatca ttgctgttca cgtaccaagt
    13501 cctgacccag aggcagatgg accttctcta agtactgtta tgtataatcc taaaattttg
    13561 agccattctg ttcaagatgc ttgtttaggg gaaggtgaag gctgtttatc tgttgatcga
    13621 gaagtaccag gatatgttgt tcgccatgcc aaaatcactg tgtcttatta tgatatgaat
    13681 ggtgaaaaac acaagattcg tttgaagaat tatgaatcga tcgtcgttca acatgaaatc
    13741 gaccatatca acggtgtgat gttctatgac cacatcaacg atcaaaatcc atttgcacta
    13801 aaagaaggcg tattggtgat cgagtagaga attggcaggt tccttcgata ctatgctaca
    13861 agaaagcttt ataaaaaagg ggagtgctca aaagctgcac ttcccttttt tatgtttctc
    13921 cactaatcga aatttagata gacatctaga aatcgcaatt ccaaaaaatt ctgtgtattg
    13981 gatacgcatc ttttcttatt ttttcttatt tcttccatat gggtgtttat ctcagagatg
    14041 atagaatagt ttctaggata aaaagtagca gctacgatgt tatccttcgt agaggattcc
    14101 tgcttcagct agtttttgtt tgatgttttt cagcatatgc ttttctgggt agcggatcgt
    14161 atgcagatgg atgccgcctg ttaattcgga taacaaagta gcattacttt tttgataacg
    14221 tctcataaag gaatcgatat cttgtggcgt tttgatgtgc aaagttcctg ctaactcacc
    14281 gtaaagggga tgttccacaa ttacatcaat gatttcacca ccattttcaa cgattagtct
    14341 cagttcttct tctgtttgat cttttccatg ctggacagcg attttactga tgtgcccagt
    14401 attggtttct tctgtttcga ggatataacc tcttgctgtt gccacgatac ttttacctgc
    14461 agctcgtagt aaagcaatat cgcctacaat gatctgacgg ctgactccaa aagttttcgc
    14521 aaacttgcta gcactcactg gtttttctgt atttgccaag gtttgcatga tttgttttcg
    14581 ccgtgtctct ccttcgatca cacttattcc tccttcattt tgttttggta ttcgcgcgag
    14641 tgtttcatca aatcaacata cgtgtctgtt attgtttcct tatatccctt atttttcagc
    14701 agactaacaa ctttatccaa tacttgctgc tctctttttg catcataaac tgcttggttt
    14761 tcttcttttt tcacttgtcc tattttagtt accgtatcca ttcttttttc taataaagca
    14821 accaactctc gatcgatcat atcgatctta tgacgtaatg tttcaagaga ctcttctgtt
    14881 agttcttcct ctactgtctt gatgatacga gcaggatttc ctgcaatcac tacattgtcg
    14941 ggaaaagatt tggtcacaac cgaacctgcg ccgactacta cgttgtcccc tagagtgaca
    15001 cccggcgtga cgataactcc tccgcctaac caaacattat taccgatttt aatgggtttt
    15061 gcgtactcca gaccgctgtt acgtttcact ggatgtagtg gatgcgtcgc cgtgtaaagc
    15121 tggacattcg gagcgaacat gcagttatca ccgatttcaa tcgtgctgac atctaaaaat
    15181 gtacaattga aatttgcata aaaattctcc ccaacaaata tattatatcc gtaatcaaaa
    15241 ttgataactg gctccatata gatttttttc cctgtacggc caaaagtctc tttcaacaat
    15301 tgggatcgca gttccgctga ttctgcttgg ttgatgattt ggctttgact tcttgcaaac
    15361 atacggtcgg caaccagctc aggatcattg gcaaaataaa gctcgcctgc aatcattttt
    15421 tctttttcgg ttttgaattc agtcataatc tgctcctaat tcttgtattt ttttgaaaac
    15481 aaccgctccg tcttcttgaa taaacgtata aggctccgct aaataaacag gcgtttgatt
    15541 gattactaag attttggctg acggctggcg aaactgtacc agatcacaaa acggatgaac
    15601 ttgaaaactc gttccaacaa tgacgatcag ctctgcttgg ctgacggcat aagccgcctg
    15661 ctccatgact ccatcttgaa acccttcacc atataacacg atgtctgggc gtatttgtcc
    15721 tccgcatgct tgatgtttgt cgctgtgaag atattctttc cagtctaccg tttgttgaca
    15781 ttttcgacaa tgacattgat acagactccc atgaaaattg acgagttgcg ggcttttagc
    15841 ctttgcgtgc aaaccgtcga tgttttggga aacaacccaa acattttttt ctttcgctag
    15901 atgtgctatt tccaaatgaa tgatattggg ttttgcttca ggatgataaa tctttttgac
    15961 aaattgatag aatttatccg gttcattcac cattgcttga tgactcagca agtattccgg
    16021 ctgctccatc ccgtggtaga ttcctgacaa agatcgatag tctggcactc cagaaggtgt
    16081 ggatacccct gcaccggtca aaaatgtaat ttgctgataa gctaatatct cttttgctgc
    16141 ttcgtctact gttaattgtt tcatgttttg tcacctcact ttccattata gcccatactc
    16201 gaacaaattt attgttgaaa agtcagaaaa atgggaataa aaaagtgtta tgggcatttg
    16261 agcaatttat cacaatgttc actgacaaaa gaatgaaaac gtgttacaat actgctgtat
    16321 aaaagtgagg tgaatcgata tgttaccatt ctttatttat tggggcgtta ttctggcttg
    16381 tatcgcctgg ttagcgttaa gcgtttattt ctctgttttt tatcttgtac gcaaagaaaa
    16441 tggaaatctt tgggcttttg ctttattcaa cgtgattgcc gcaattgttt tagccattac
    16501 attagccgta tatcgaacat ggggctgggg aatcacccaa tactcaagtt tgatttatct
    16561 aatattagcc atttatggcg tcgttgttct tttgcaagcg atcctaggac gtgagccaaa
    16621 gaaagcggca gcttaaatga tcgagatgga actggacaca ggaaattttc tgttctagtt
    16681 ctttttttat tgtattacat acatttttgt gtataattat actatcgttt gcaatggata
    16741 gatgattcac attttgattg ttttttcggt aaaagtaaag atttctatat ttatcagtat
    16801 tttttcaaaa accagcagtt atagattgac aaagcctctc gagtatcgta aacttttttt
    16861 gtataaataa ctgaatacga gatgcctcaa tcaatgaggt agaggtcgcg agaatcatta
    16921 accctgtgga gtgaagcaaa catggagaag caggaatagg gaggatcgcc gaaatgtgca
    16981 cttttttgct tggagtgtat gttgggacgc aagttaagag cttgcggact gtcttagcag
    17041 tgatgctaag ttgcgctatg ttttgtgaga ggtttatcac cttatggaat tttagcggcc
    17101 tttttatatc tataaatcgg tcgcttttta ttttttcaga taaagcttta ccgcatttca
    17161 tctagaccgc tcgtgtatca tataatcaga aagggagaaa aaaatgaaaa aaaatatggt
    17221 ttcagttgcc gtactatttg cttttgtctt ctcactattc agtgtgggat ctctggcaaa
    17281 tgcagatgaa aaaacaccga gcgatgagtt tcgcgtaggt atggaggctg ggtatgcacc
    17341 cttcaactgg tctcaacaaa cagatgagaa taatgccttg ccaattcaag gccaaaatag
    17401 ttacgccggc ggttatgatg ttcaaatcgc taaaaaagta gcagatggtt tagggaaaaa
    17461 attagtcatc gtccaaacga aatgggatgg tctggctcct gcccttcaat caggcaagat
    17521 cgacgcgatc atcgccggaa tgagccctac tgctgaacga cgaaaagaaa ttgattttac
    17581 cgatccttat tacgaatcac aattagtagt agtagttcaa aaagatagca aatatgcaaa
    17641 agcaaaaagt ttagcagatc tttctggagc aaagatcact gctcagttaa atacattcca
    17701 ctatactgtg atcgatcaga taccagatgt gcagaaacaa caagcaatgg ataatttctc
    17761 agcaatgaga acagctttag cttctggaat gatcgatggt tatgtcagcg aacgtccgga
    17821 aggtgtaacg gcaactagtg tgaacaaaga cttggaaatg cttgaattca gcggtgcaga
    17881 tggcttccag acaaacccgg aagatgttca gatttctgtt ggtatgcgta aaggcgaccc
    17941 ggatatccaa aaagtaaacc agattctttc tggcatctct aacgatgaac gaaccaaaat
    18001 tatggaccaa gcagtcaaag atcagcctgc tgcaactgat agtgaagaaa aacctggcat
    18061 catttctgat ttcactacga tttggaaaca atacggcaat atgttcttac gaggtgctgg
    18121 acttaccttg ttcatcgcat tgatcgggac agttgtcggt actacacttg gattactgat
    18181 tggtgtcttc cgttcggtac ctgaatcagg aaatgcactg actcgcttct tccaaaaaat
    18241 cggaaatgca cttctttcta tctatgtcga agtattccgc ggaacaccga tgatggtaca
    18301 agcaatggtc atcttctacg gtctggcact cgcttttggc atttcattga accgaacgat
    18361 tgccgctttg tttattgtct ctctcaatac tggagcttac atgacagaga tcgttcgtgg
    18421 ggggattttt gcagtagatg aagggcagtt cgaagctgcg caagccattg gtatgaccca
    18481 tggccaaacg atgagaaaag ttgttattcc acaagtttta cggaatatct tgccagcaac
    18541 aggaaatgag tttgttatca atatcaaaga tacagcagtt ttaagtgtaa tcggcgtagc
    18601 tgatttgttc ttccaaggaa actctgcttc cggtgcgaat ttccaattct tccaaacatt
    18661 tacgatcgtc gggatcatct acttagtcat gacctttacg atcaccagaa tcctgcgggc
    18721 ggtagaaaag aaaatcgatg gtccttcagc ttataccaag cttgaagaaa tcgacaattc
    18781 aaatttgcag gaataaggag gaagaatcat gaatgaaaaa attattgaaa tcaaacatct
    18841 aagaaaaagc ttcggtgaaa atgaagtatt gaaagatatc agcgtaacag tcaataaagg
    18901 tgaagttgtg acgatcatcg gttcttccgg ttctggtaaa tctactttct tgcgctgtat
    18961 caatttactg gaaaaaccaa caggcggcga gatcatttat aatggagaaa atgtattagc
    19021 tcctaaatat aatttaccta aatatcgaac gaatctaggt atggttttcc aatccttcaa
    19081 cctgttcaac aatatgaatg tcttggaaaa ctgtatgtct ggtcaaacaa ctgtcctgaa
    19141 acgagataaa gaaactgcca aaaaagtagc aatcgaaaat ctggaaaaag ttggtatgga
    19201 tcgttatatc gaagccaaac cagcacaatt atctggtgga cagaaacagc gtgtcgcaat
    19261 tgctcgggca ttatcaatga atccagatgt attgctattc gacgaaccta cttcggcgtt
    19321 agaccctgaa atggttggcg aagtgcttaa tacgatcaaa gacttagcac atactggctt
    19381 gacaatgctt atcgtgacgc atgagatgga atttgcaaaa gatgtatctg accgtgtcat
    19441 ttttatggat aaaggtgtga ttgcagaaga aggtacacca gaagatatct ttgtccatcc
    19501 gaaagaagaa cgtacaaaag aattcctttc acgtatttta aatgcttagg atcaatagct
    19561 gattgggaca aagagctgcg gtgtttaccg cagttctttt ttatgaatta ctcataaaag
    19621 gtaaatttct gaaaattttg gaatcttagt tgtgtttttc agaaaactct cttaatatgg
    19681 aagtaattaa acttttttag ttaggatgat ttaaaaatga atgaagagca agaactgcag
    19741 cgtggactga aaaatagaca tgtccaactg atttctattg gcggagcgat tggaacgggg
    19801 ttgtttttag gttccggaaa gtctatccag ctagctggcc cttcgatatt gctcgcctat
    19861 cttattactg gatgcatctg ttttttcatt atgcgtgcgt taggcgaact tctgttatca
    19921 aacaccaata accattcttt tctggatttt gttgcagaat atcttggcaa aaaagcggct
    19981 ttcatcactg gctggacgta ttggttctgc tgggtatcga ttgcaatggc cgatttaaca
    20041 gccatcggaa tgtatgtacg ctactgggcg cctggtgttc ctcagtggct tcctgaactg
    20101 attgcattag gtcttcttct ttgcttgaat atggtggctg tctcattgtt tggtgagtta
    20161 gagttttggt ttgcattgat caaagttgta gcgatcattg cctttatcct cgtaggagct
    20221 tatatgatcc tcacccacta tacgacagat attggcacag ccagtatcac gaatctatgg
    20281 tctcatggcg gattctttcc aactggtgca aaaggattta tcttagcttt ccaaatggta
    20341 acctttgctt ttgctgggat agaattagtt ggattagtcg ctggagaaac agaaaatcct
    20401 gaaaaagtgt tgccggaagc tatcaataat attcctatca ggatcatctt attttactta
    20461 ggatctcttt ttgtcattat ggcaatctat ccatggaaca gcttgaatcc agataacagt
    20521 ccatttgtag aagtattttc ggaaattggg atcacgattg ctgcttcctt gatcaattta
    20581 gtcgtattaa gtgcagcagc ttctgcctgc aacagtgcaa tatatagtac aggacgtatg
    20641 cttcgttctt tagcgcaaga aggcagtgcc cctaaaaaat tcaaaaagtt aacaacacat
    20701 catgtgccag ggaatgcttt gacattttct actatcgtca ttttcatttc tgttatcttg
    20761 aattacgtga tgcctagtga agtattcaca ctagtaagca gtattgccac tacttgtttc
    20821 ttgtttatct ggagtatgct tgtctacacg cacatgaaat atcgtaaaag tatcctcggc
    20881 aaaaaagctc attcatttaa aatgccgttg tacccattta gtaattatct ggtatttctt
    20941 tacatggcct ttgtgtgtgt cgtcttgttc ctaggaaaag atacgagaat cgctttatta
    21001 ctaacacctg tgtggttctt gcttctatta ctaatctatc atatgaaata tgaacgtaag
    21061 aagtaagaaa gtagttgtga cagaagtggt cagcttcgag aaataagaag gaatttacga
    21121 aaattgcttc ttaaattttt gtgaaatttc ggcttatttc ttggagataa aagcttctgt
    21181 cccagcctct tttctatttc cccggaaaca accgctgata ataggcatct gcttttttat
    21241 aggaaaaaat ccaaatacca gatacaaaaa aacgtccgaa taacccgatc aacggtacaa
    21301 aaaagacacc ggcaatcaag cataagttaa tcatcatgga gataagactt cgacggtcct
    21361 ttgacaaaac tctcacgaca tgggaatctg gtttatttgc tttcatgatt tgcctcatca
    21421 agataccaaa agaaaaactc cacatcgtgt atactaagac ataagccatc tcaggccaaa
    21481 aagcttcagg gttacgcccg acccaactag taacagaagg acataaagtc agccaaaaga
    21541 gcaaaaagat attcgaccag accactcggt aattaattcg ttctgccatc tgaaatatac
    21601 gatgatggtt cacccagatc accgcaacaa atataaagct gatggtataa gctaataatg
    21661 tatttcggat gacccataaa gaatgaaagc tagatgtatt agggatcttg atctctaata
    21721 caataactct caaaacaatg gctaccactc catcagtaaa tgcttctaat ctcgactttg
    21781 tcacgatgat tcctcctcag atgattcagc gcttttattt tatcacttac gttattaaat
    21841 aaccatcgtt ttatataggg aattttctga aaattgttgc atatttttcg aaattttgtt
    21901 acttttaaaa aa
//
LOCUS       DARBET010000042        21871 bp    DNA     linear   BCT 07-NOV-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4027
            SAMEA114569841-rid20112183.denovo.042, whole genome shotgun
            sequence.
ACCESSION   DARBET010000042 DARBET010000000
VERSION     DARBET010000042.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA114569841
            Sequence Read Archive: ERR12203666
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 21871)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 21871)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001972618.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001972618.1
            Genome Coverage       :: 132x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/07/2023 13:18:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,041
            CDSs (total)                      :: 2,975
            Genes (coding)                    :: 2,908
            CDSs (with protein)               :: 2,908
            Genes (RNA)                       :: 66
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 67
            CDSs (without protein)            :: 67
            Pseudo Genes (ambiguous residues) :: 0 of 67
            Pseudo Genes (frameshifted)       :: 26 of 67
            Pseudo Genes (incomplete)         :: 28 of 67
            Pseudo Genes (internal stop)      :: 20 of 67
            Pseudo Genes (multiple problems)  :: 6 of 67
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..21871
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA114569841-rid20112183.denovo.042"
                     /isolate="VRE4027"
                     /db_xref="taxon:1352"
                     /country="Denmark"
     gene            complement(205..1104)
                     /locus_tag="SCJ86_001748"
     CDS             complement(205..1104)
                     /locus_tag="SCJ86_001748"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348747.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphohydrolase"
                     /protein_id="HEG1169289.1"
                     /translation="MGKLAIISDLHVDINKFGESELMQLWQVLQKHKITRLHLAGDTA
                     NKVDRCLAVVEFFVNKGLSTTFHFGNHELADVPNEQMMDHFPDPHFLNERYIALNEQT
                     VLLGMNGWYDYQFSELKDYEAIVRMKRLYWYDRFIQRGKTDLEVNAEMLAAAKNLLDQ
                     LEAKKLEVILSTHFVPKRAFIMYQSAPYERWNKLNAFLGSESFGELLDRYSNIKQVVF
                     GHTHRRFEDKSIGHTIYSCRPFGYYYEWQLTRDFVLKEQLLDSYDPMKLRVLLRAHHP
                     AFQAYQTQHLQEEFEQAMTIVPY"
     gene            complement(1114..1470)
                     /locus_tag="SCJ86_001749"
     CDS             complement(1114..1470)
                     /locus_tag="SCJ86_001749"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313945.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-sulfur cluster biosynthesis family protein"
                     /protein_id="HEG1169290.1"
                     /translation="MKLEVTKEAQEVLKNKLEADDQLLLDIENGDGPFAESQVSCQLD
                     TAFRLIIVKKDTQTDLSLYSVVADTPVGPIKIKDSAILYMDDPSTLALEPTYNSLQLK
                     GPSGLRKGNLQVVRKD"
     gene            1825..2121
                     /locus_tag="SCJ86_001750"
     CDS             1825..2121
                     /locus_tag="SCJ86_001750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315522.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phenylalanyl-tRNA synthetase subunit alpha"
                     /protein_id="HEG1169291.1"
                     /translation="MKEYIKNIYFIEETQNIEGSYIEVKTLFVNEDKTKALDIYKKLA
                     SKKTNSFGLILSEYKIKAEESYFYQLLKRWSKLPADFYRKMQIINYQPLAETHA"
     gene            complement(2765..3718)
                     /locus_tag="SCJ86_001751"
     CDS             complement(2765..3718)
                     /locus_tag="SCJ86_001751"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287908.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="siderophore ABC transporter substrate-binding
                     protein"
                     /protein_id="HEG1169292.1"
                     /translation="MKKLVVGILILASFGLAACGNSNNTSQADTKKSSTQTETTLTIT
                     DSNGDQIEVPNNPEKVVVFDNGSLDTMDALGVGDKVVGAATSSLPEYLSSYKKVESAG
                     GIKEPDLEKINQLQPDLIIISGRQRDFQKDLSQIAPTIFLSLDAKNPWESFQQNVTAL
                     GEIFGKQEEAKTQLEELSSAIDQTKKKAEATDKKALVTLVNEGQLSAYGSGSRFGFIH
                     DLFGFEQADDQIEASTHGQSVSYEYVLEKNPDILFVVDRTKAIGGDDSKDDISANELV
                     AQTNAGKNQQIISLEPDVWYLSGGGLESMKLMIEDVNQAFK"
     gene            complement(3741..4496)
                     /locus_tag="SCJ86_001752"
     CDS             complement(3741..4496)
                     /locus_tag="SCJ86_001752"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002372520.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HEG1169293.1"
                     /translation="MIDLQKVNKLYHGKSVVKDVQLTIPEKKLTAFIGPNGAGKSTVL
                     SMISRLIPKDTGEIYLDHNEVKAWKSNELAKKLSILRQSNEINVQITVKELVEFGRFP
                     YTKGKLTTKDEEIVQESLEHLGLEEIADQSIDTLSGGQLQRAYIAMVLAQDTDYILLD
                     EPLNNLDMNFAVQIMQILKNLVQEIGKTIVIVLHDINFAASYADEIVAMKDGQLFTQG
                     TTQEMIKKDVLDELYEMDIRICEIEGRRFCLYF"
     gene            complement(4493..5452)
                     /locus_tag="SCJ86_001753"
     CDS             complement(4493..5452)
                     /locus_tag="SCJ86_001753"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron chelate uptake ABC transporter family
                     permease subunit"
                     /protein_id="HEG1169294.1"
                     /translation="MLKKKPSIFIGMLVLVLFVTGMYLTIHTYGNWHFACALRSKKIF
                     AFILVSLAVSSATISFQTITHNQFLTPSVLGLESLYVLVQTVLFFFIGGVTMLQQTGT
                     VFFLLNVGLMVGLSLLLFFFLLKKMRANLFLLLMSGMILGTLFGSVSTFLQVIMDPNE
                     YDLLQGRLFASFGNIQSQYLLPASLIIIGCWYLLFRYRHVLDVFHLGNEQAVNLGIDT
                     DRFEFLILCCVSLLTGTSTALVGPVTFLGFIVANISYRVFSTYRHGQLFIGSFLVAML
                     FLTGGQLAVEQIFGWNTTVSVVVEFVGGIYFVTKLIRERKSIL"
     gene            complement(5445..6395)
                     /locus_tag="SCJ86_001754"
     CDS             complement(5445..6395)
                     /locus_tag="SCJ86_001754"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341944.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron chelate uptake ABC transporter family
                     permease subunit"
                     /protein_id="HEG1169295.1"
                     /translation="MRYGRLVALLVILSSISLFIGVSNVQLSELLQLDGQSLQILLVT
                     RLPRTICLILVGATSSICGLIMQHLTQNKFVSPTTAGTMDSARLGILVAMIFLPGASL
                     IVRSLTAFCFAFAGTLLFLSLTRLFLQKNQLMLPLIGVMLGNIIGSVATFFAYQFQLV
                     QNMTSWLQGNFATVIRGNYELLYAVVPLLLLVWLFAYRFTIIGLGESFAVNLGIDYQK
                     MQFLGIGLVSLASAVMLIMVGSIPFIGVIIPNLVSRIYGDQVHKTIGVTALAGSLFLV
                     FCDLVARVLIFPYEIPVSVIVGIIGGAIFLYLLIRGRRYA"
     gene            6545..6997
                     /locus_tag="SCJ86_001755"
     CDS             6545..6997
                     /locus_tag="SCJ86_001755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YueI family protein"
                     /protein_id="HEG1169296.1"
                     /translation="MAQDDVKSHLDKGLYGTPLVNPEEQHKYLGTFRERCYLSMTVAE
                     MIQPKNKENFLKELKKHPDASILLNGALPLSVQNQYIQLATKNNSRFTVVNDYVTANP
                     EEFGLLLTAKEAVNEPVIDIEKKYPEENSSPAEKEKPKKKKSFWDRLF"
     gene            complement(7086..9353)
                     /gene="gshAB"
                     /locus_tag="SCJ86_001756"
     CDS             complement(7086..9353)
                     /gene="gshAB"
                     /locus_tag="SCJ86_001756"
                     /EC_number="6.3.2.2"
                     /EC_number="6.3.2.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional glutamate--cysteine ligase
                     GshA/glutathione synthetase GshB"
                     /protein_id="HEG1169297.1"
                     /translation="MMNFKQLLLHVNARPFIDQARFGIEREGQRVDLAGNLAKTDHPA
                     IFGDRSYHPYIQTDFSETQTEMITPVTDSIPELFQYLAAVYDVTARSIPKEEMIWPLS
                     MPPALPEKDEEIIIAKLKNFEDVLYRRYLAKEYGKRKQMVSGIHFNFEFGDELLRTLF
                     SHQEEFQDFSEFKTELYLKTARNFMRYRWMITYLFGASPMSEKNYFLDESHPQEPVRS
                     IRNSALGYTNHPNVKVSYASMKQYLADIERMIEEGKLSEEKEFYTPLRFRGGKKVADL
                     ATTGVRYIELRNIDLNPYARLGINPEQVRFLQLFLMYMLWTEEKEDCDQWVAEGTTRN
                     NKVALEQPSDQTEFHQEGREILEGMKQMLVELDWLDSLYLVEEALTQMDHPEQTLAAK
                     LYQEAQLSSQQEVAVALGHQYYKESHERPYQLAGFREMELSTQIFMFDAIQKGVQVKV
                     LDESDQFLRLQFQDHVEYVKNANMTSKDSYIVPLIMENKTVTKKVLKEAGFRVPGGAE
                     FSSMEEAVKAYPRFAEQAFVIKPKSTNYGLGITIFKEGASLEDYQAGLAIAFREDSSV
                     LVEEFMPGTEYRFFVIDGEVQAIMLRVPANVIGDSIRTVKELVEEKNSDPLRGTNHRA
                     PLELIQLGELEQLMLKEQGLTIESVPQANQIVYLRENSNISTGGDSIDMTDEFSEAYK
                     KIAVSAVEALGAKISGIDLIIPDKEIDPTTDKKAYGIIEANFNPAMHMHVYPFAGKGR
                     RLTMNVLKLLYPEVF"
     gene            complement(9540..10538)
                     /gene="ftsY"
                     /locus_tag="SCJ86_001757"
     CDS             complement(9540..10538)
                     /gene="ftsY"
                     /locus_tag="SCJ86_001757"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348059.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="signal recognition particle-docking protein
                     FtsY"
                     /protein_id="HEG1169298.1"
                     /translation="MGFFDKIKRAFTGEEKTEEVEVQEKYDKGLEKTRKTFGERMNEL
                     FANFRTVDEDFFEELEETLIGADVGFETAIRITEALRQEVKLRNAKKSAEVQNAIIEK
                     LVDLYEAEGINEVNELNIQEDGLTVILFVGVNGVGKTTSIGKLAHQFKNEGKKVLMAA
                     ADTFRAGAIDQLVVWGERAGVEVVRGNAGGDPAAVVFDALERAKTEQADVLLVDTAGR
                     LQNKVNLMNELEKIKRVIQRELPEAPQETLLVLDATTGQNAMVQAKQFKETTDVTGLV
                     LTKLDGTAKGGIVLAIRNELHLPVKLVGLGEGIDDLEPFDPNDFVVGLFKGLLKEE"
     gene            complement(10549..11358)
                     /locus_tag="SCJ86_001758"
     CDS             complement(10549..11358)
                     /locus_tag="SCJ86_001758"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853156.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cof-type HAD-IIB family hydrolase"
                     /protein_id="HEG1169299.1"
                     /translation="MIKLVAIDLDGTLLNEQKLISDENKQALAQAKQQGVKIVLCTGR
                     PLAAMAHYLQELGLVDEGDYSITFNGGLVQKNDTGEIMEKKVMEVSDIQRLYKLAQQL
                     DLPLDVLSDNVVLQLPSAPRKQSLYNVLNKLLQFQPADISDITDEFVLNKAVIAYDQE
                     ELDPKIKEIPAEFHEMYEIIKTRSMLLEFMPKGVTKAYGISLLAKDLGLKQEEIMAIG
                     DEENDLPMIQYAGLGVAMENAVPFVKEAADFVTASNEENGVAKVIQKFVLE"
     gene            complement(11367..14948)
                     /gene="smc"
                     /locus_tag="SCJ86_001759"
     CDS             complement(11367..14948)
                     /gene="smc"
                     /locus_tag="SCJ86_001759"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287897.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chromosome segregation protein SMC"
                     /protein_id="HEG1169300.1"
                     /translation="MYLKRIEIAGFKSFADKTVIDFENSVTAVVGPNGSGKSNITEAI
                     RWVLGEQSAKSLRGGKMPDIIFAGSDTRKQLNIAEVTVILDNSDHYLPLEYNEISVTR
                     RYRRTGESEFFINKQSCRLKDIQELFLDSGLGKESFSIISQGKVEAIFSSKPEDRRGI
                     FEEAAGVLKYKQRKKKAEQKLFETEDNLSRVQDIIHELEEQLTPLAAQSEAAKEFLRL
                     KETLTQTDVSLMVAEIKTAKKDWDNKQAQLAKFNLELGKLSESIQEQESILAKQRKEN
                     AQADRLIEKNQQVLLDLSEKLKQTEGQKDVLQERTKHTQKSSQEYQTSLAEAQKKVKH
                     FEKLQESLMKAAAEKETEIQKAEANLIKTQQELEKYQKSTKELLAELRDQYVDLMQEQ
                     AAVGNELKYLERQYIQETAKSKQTLAKQSEVEASVDRLILQKEELTQKQAQLKSSLTE
                     TKEKLEMIQQNGKKFQEALAKEQPKMYQLMNQVQQLRARKKSLQEIQENYFGFYQGVR
                     LVLQHKQQLSGIVGAVAELIDVPADFTLAIETALGGAAQHVIVENEKDARQAITYLKQ
                     QRGGRATFLPLTTIKPRQLPAHILTQAAAVEGFIGIASEQVSYPDQIQTVVQNLLGTI
                     LLAKDLTSANAIAQTIRYQYRVVSLEGDVMNAGGSMTGGANKRGNQGSLFVQNQELKQ
                     LTSEFEEADKQLQAQEKKVQELQQETARLAEEQEVLRTRGEQLRFEEQEATNQLQNII
                     NELERFEKEKQISTFETRELQQFIEDYEKQTNELKDKQTDLESQRQKIDEEIKSLSQE
                     SDQMEARRAQVQSQKAQEQADLAVLKEQFNHLQIQLRGARVQKAEATERQEAIEKQLA
                     TLTADFSDHEVTEESLEKQINELSAQRETLKAELAKAKEQRDRRQKEIDKLEAVLAER
                     NREQKARLSEQSKLEVQKDRAEMILDNHLTYLQSEYQLTFEKASQDYQETTDIEDSRT
                     KVSSLKEQIEKLGPVNLNSIEQYEQVSERHTFLATQRDDLLAAKNQLFETMDEMDDEV
                     RTRFKEVFEAIRQEFKIVFPNMFGGGKAELVLTEPTDLLKTGIEIEVQPPGKKLQSLS
                     LLSGGERALTAIALLFSIIRVCPVPFCILDEVEAALDEANVKRFGRYLSDFQDDTQFI
                     VVTHRKGTMVAADVLYGVTMQESGVSKIVSVRMEDINEEAKINETKV"
     gene            complement(14968..15654)
                     /locus_tag="SCJ86_001760"
     CDS             complement(14968..15654)
                     /locus_tag="SCJ86_001760"
                     /EC_number="3.1.26.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177197.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease III"
                     /protein_id="HEG1169301.1"
                     /translation="MDNQLTKELKENYNIVFHDLNLLEQAFTHSSYVNEHRNLQLSDN
                     ERLEFLGDAVLELMVSQYLFRLYPHLPEGKLTKTRAAIVREDSLSKFAKECHFDQYVL
                     LGKGEENSGGRTRPALLCDLFEAFLGALYLDQGFDTTHAFIEKVIFPKVKAGAFSHEM
                     DHKTKLQEVLQKSGDVSIEYRLINEEGPAHERVFWIEVYVDDQLIGTGQGKSKKLAEQ
                     AAAENALAAL"
     gene            complement(16002..17783)
                     /locus_tag="SCJ86_001761"
     CDS             complement(16002..17783)
                     /locus_tag="SCJ86_001761"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008265999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oligopeptide ABC transporter substrate-binding
                     protein"
                     /protein_id="HEG1169302.1"
                     /translation="MKKTIFGFAALMSAVVLAACGGGGTDTSGNNSGGNGNNKEELTF
                     PIATKNNDDAIEDGELEAAVATDSQFKGLFQWEFYQDSYDAAFMAPSHEPLFANDDDF
                     RITDDGAATLDLDEDTKKATIKVKDNVKWSDGEDVTADDVIFPYEIIGNKDYTGIRYD
                     DTFRNIVGMEEYHDGSADTISGIKKIDDKTVEIEYKEVNPSMLQSGGGIWSYAAPKHT
                     LEGVAIKDMESSDQVRKNPVTFGPYYMSNIVAGESVEYLPNEHYWNGTPKLKKITMKS
                     VPMASATEALNSKLYDMIFQMPTDTYDTYKEIAGYTNLGREQTSYTYLGFKLGKWNAD
                     TKSVEYNPDSKMADKSLRQAMGYALDNNAVGERFYAGLRSNATSLIPPVFQSYHDDEM
                     KGFTQDLDKAKKLLDDAGYKDVDDDGLREDKDGNKLTINFASMSGGETAQPLADYYIQ
                     QWKEIGLDVQYTTGRLIEFNSFYDKLENDDPEIDVYQAAWNTGTDPNPSGLWGPNAAF
                     NYTRYESEENTKLINDISSSKAFDEEYQKEAYQKWQQYAFEEAFAIPTLFRNEVLPVN
                     DRVKDWDWSYDVPNPWANVSVTADSRS"
     gene            complement(17809..18723)
                     /locus_tag="SCJ86_001762"
     CDS             complement(17809..18723)
                     /locus_tag="SCJ86_001762"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287893.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEG1169303.1"
                     /translation="MSEKNNTAVQESIPPMGIRMIAREFKKDKIAIFSLGLLVVLLLV
                     IFIGAMLTDQDKVMYVSIFDKYAAPGTVSTQGQKFILGADEGGRDVLGQLIIGARNSV
                     TIGFAITILTSIIGVGLGIISGYYGGIIDNILMRIVDFIMILPIMLIIIVFVSIISRY
                     NVWSFIFIMSAFYWVAKARLFRSKTLSEARRDYVSASKTMGTNDFKIMFREIMPNLSS
                     LIITNLTMNFAANIGIETTLTFLGFGLPANVPSLGTLIGYASNGEVLVNRQWIWLPAS
                     ILILVLMLSINYVGQAFKRSADARQRLG"
     gene            complement(18750..19712)
                     /locus_tag="SCJ86_001763"
     CDS             complement(18750..19712)
                     /locus_tag="SCJ86_001763"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010750774.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEG1169304.1"
                     /translation="MWKTILRRVLLMIPQVIILSVLIFLLAQAMPGDPFTGLINPNQD
                     PAVIEQMRQAAGLNDPWYVQYFRWIGNALQGDFGQSFLYKRSVADLIGERIINTLYLS
                     ILTVVLTYLIAVPLGMLAGRYQNSILDKIVVIYNFFSFAVPLFIFGLIMLFVFGYRLG
                     WFPTSGSQTLGLQGGFITQWIDRLKYILLPSITQAFLATAVTIQYLRSEVIDSKSLDF
                     VRTARSKGVPTNKVFSRHIFRNAALPMASQMGYEITSLIAGSVVIEKIFGYPGVGKLF
                     IDSIGQRDYTVITSLVLILGIATLVGTLLSDIIMSIVDPRIRIQ"
     gene            complement(19712..20656)
                     /locus_tag="SCJ86_001764"
     CDS             complement(19712..20656)
                     /locus_tag="SCJ86_001764"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297777.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEG1169305.1"
                     /translation="MTELITIKDLKVHYPIRSGFFNRVTDHVYAVDGVDFIIEKGKTY
                     GLVGESGSGKSTTGKAVVGLEKVTAGQIMYEGKDVTKRSNRKKMGYNKDVQMIFQDSM
                     SSLNPKKRVLDIIAEPIRNFERLSDQEEKKKVKGLLDIVGMPEDALYKYPHEFSGGQR
                     QRLGVARAVATNPKLIVADEPVSALDLSVQAQVLNFMKRIQQEYGLSYLFISHDLGVV
                     KHMCDNIAIMYKGRFVEIGTREDIYNDPRHIYTKRLLSAIPQIDVENRESHKENRRRV
                     EQEYIQNQKEYYDATGRVYDLRTITTTHKVALKDGGAS"
     gene            complement(20657..21661)
                     /locus_tag="SCJ86_001765"
     CDS             complement(20657..21661)
                     /locus_tag="SCJ86_001765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736807.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEG1169306.1"
                     /translation="MSDNQLLEVQNLHTGFRLKDDYYDAVDDVSFTLDKNEILAIVGE
                     SGCGKSTLATTIMGLLDPNNTKITGEILYNDLNLIDLNETLYNKIRGNDIGMIFQDPL
                     SALNPLMRIEDQIKEGLSYHTKMNAEQRQARALELLEQVGIPNPARVGRQYPHELSGG
                     MRQRVIIAIAIACKPPIVIADEPTTALDVTIQAQILDLLKDLQEETKTGIILITHDLG
                     VVAEMADRVAVMYGGQFVEVAGVKELFDHPKHPYTRSLLNSIPQEGNHDAELHVIEGV
                     VPSLKNMPRKGCRFAPRIPWIPASDHEEEPILHEVAPNHLVRCSCYKHFHFRDEKGEV
                     "
BASE COUNT         6124 a         4656 c         3863 g         7228 t
ORIGIN      
        1 tatatttttt tttgtgatta tcgttcaaaa aaagaataca cgcttgcatc tagtactctg
       61 cgagttacaa atcatccgat caaataaaca ttcgctgatt attttccgca aaaaaagcag
      121 catatccacg ataaatggat ctactgctct tacttatcct tatgttttac aaggtatttt
      181 cttttacaca ttactaatgc tgtctcaata cggaacgatt gtcatcgctt gttcaaattc
      241 ctcttgcaaa tgttgtgttt gataggcctg aaaagctggg tgatgggcac gcaagaggac
      301 acgtaatttc ataggatcat agctgtctag caactgttct ttcaagacaa aatctcgggt
      361 cagctgccat tcataataat aaccaaaagg tctgcagctg taaatcgtgt gaccgatgga
      421 tttatcttca aagcgacgat gcgtatggcc aaaaactact tgtttgatat ttgaataacg
      481 gtcaagtaat tctccgaatg attctgatcc taaaaatgca tttaatttgt tccaacgctc
      541 ataaggagca gattgataca tgataaatgc ccgtttagga acgaaatgag tggataaaat
      601 cacctcaagt ttttttgctt ccagctggtc tagcaaattt tttgcagctg caagcatttc
      661 tgcattgacc tcgagatctg ttttcccacg ctggatgaac cgatcatacc aatacaaacg
      721 cttcattcgt acgattgcct cgtaatcttt caattcggaa aactgatagt cgtaccagcc
      781 attcatccct aaaagaacgg tctgctcatt taaagcaata taccgttcat ttaaaaaatg
      841 tgggtctgga aaatggtcca tcatctgttc attgggcaca tctgccaact cgtgattgcc
      901 gaaatgaaat gtcgtagata atcctttatt gacgaaaaat tcgacaacag ctaggcaacg
      961 gtctacttta ttagccgtat ctccggctag atgaagccga gtgatcttgt gcttttgcaa
     1021 aacttgccaa agctgcatca gctctgattc gccgaattta ttgatgtcca catgtaggtc
     1081 gctgatgatc gccaattttc ccattttatc cctctagtct tttcgaacaa cttgcaagtt
     1141 gccttttctc aagccgcttg gtccttttaa ttgtaaacta ttataagttg gttctaaagc
     1201 aagtgtggat ggatcatcca tgtaaagtat tgcactgtct ttgattttga ttggtccaac
     1261 tggtgtatca gccactacag aataaagact tagatctgtt tgtgtatctt ttttgacaat
     1321 gatcaatcga aatgccgtat ccaattggca actgacttgg ctttctgcaa atggaccgtc
     1381 accgttttcg atatctaata aaagttgatc gtctgcttct aatttattct tcaatacttc
     1441 ttgtgcttct tttgtcactt ctaatttcat ctgtatcacc tcatgtcata gaataatcac
     1501 tttattcatc agatgcaact aatttgtctg gttctgactg ttctcaattt attggaaagt
     1561 aagtcattcg tttattatga tttctcttat cacaaaagag acaactttcc cttttcttcg
     1621 ttcagtttct tgaacggaaa aagacccaaa aagcaacctg ttatttgttt aaccttattc
     1681 ctttcttaaa taacaaaaaa tactataaaa aataaaaaac ctttaacaac tattggaaaa
     1741 agaattactt tttgttcacg gattattaac aactcttcgg tagagtaaac ttatcaaata
     1801 aaagacttct ttggaggaat cactatgaag gaatatataa aaaacatcta ttttatcgaa
     1861 gaaacacaaa atatcgaggg ttcttatatt gaagtaaaaa cactctttgt caatgaagat
     1921 aaaaccaaag cattggatat ttataaaaaa ttagcttcaa aaaaaacgaa tagttttgga
     1981 ttgatattaa gcgagtataa aatcaaagca gaagagagtt atttctatca attattaaaa
     2041 cggtggagta agcttcccgc cgatttttat cgaaaaatgc agatcatcaa ctatcaaccg
     2101 cttgctgaga cacatgccta aatagaggtt gtgaaatcag tgctttgagc taataccgaa
     2161 ggtcagctga ttgaacattg tttagaagag attgtgacaa ctgcgatttg ctttgagtaa
     2221 taagaacgct ggacagaaaa tagcttctca tattttctag tctagtgggc ttattgccga
     2281 gaagcagcag atggaacatc gtttagaaga gattgtgaca actgcgattt gctttgagta
     2341 ataagaacgc tggacagaaa atagcttctc atattttcta gtctagtgga cttattgccg
     2401 agaagcagca gatggaacat cgtttaaaga agagatcggt acaaaataaa aatatctaaa
     2461 tttaagtttt atctaagttt cgctttgtac ctttgattca aatcaaagga ggcgatcttg
     2521 atgctgaaaa aatggatcac actatttttt gccactcccc attctagaat caacccgtat
     2581 gatgtagata atgaattcac gttgaaaaaa gatttctcaa ataaataaaa accatccgta
     2641 aaatcatcaa tacgatttta cggatggttt ttgataactt ctttgaggat aggctcttgc
     2701 tatttaagaa tgtaagaaac attattgtta catagtatga gagtttaatc ctctgttatt
     2761 tatgctattt aaatgcttga ttcacatctt cgatcatcag tttcattgat tctaaaccgc
     2821 ccccgcttaa ataccagaca tctggttcaa gggagatgat ctgctgattt ttaccagcat
     2881 tcgtttgtgc taccagttca ttggcagaaa tatcatcttt gctgtcgtct ccaccaatag
     2941 cttttgttct gtctactaca aataagatat ccggattttt ttccagcaca tattcataag
     3001 aaacactttg accgtgagtg gatgcttcga tttgatcatc tgcttgttca aagccaaata
     3061 aatcatggat gaaaccgaat ctagaaccag atccgtaggc tgataattgc ccttcattca
     3121 caagtgtgac tagtgctttt ttgtcagtag cttctgcttt ctttttcgtc tgatcgattg
     3181 cactagataa ttcctctaat tgagttttag cttcttcttg cttaccaaag atctccccta
     3241 aagcagtgac attttgctgg aaagattccc atggattttt cgcatctaaa gaaaggaaaa
     3301 tggtcggagc aatttgactt agatcttttt gaaaatctct ttgtcggcca gaaatgatga
     3361 ttagatctgg ctgaagctga ttaatttttt ctaagtctgg ttcttttatt ccacctgctg
     3421 attctacttt tttataggat gaaagatact caggaaggct gcttgtagct gctccaacta
     3481 ccttatctcc aacaccaaga gcatccatcg tatctaaaga accattgtcg aatacgacga
     3541 ctttttctgg attatttggc acctcgattt ggtctccatt actatctgtt atcgtcaatg
     3601 tagtttcagt ttgtgtgctg cttttttttg tgtctgcctg actggtgttg ttgctatttc
     3661 cacatgctgc taaaccaaat gatgctagaa tcaagatacc tactactaat tttttcatct
     3721 cttctctcct aatgttagtt ttagaaataa agacagaatc tgcggccttc aatctcacaa
     3781 atccgtatgt ccatttcgta taattcatcc aatacatctt tttttatcat ttcttgggtc
     3841 gttccttgcg taaataactg tccatctttc attgcaacaa tttcgtctgc ataacttgca
     3901 gcaaagttga tatcatgaag aacaataaca attgttttcc cgatttcttg tactaaattt
     3961 tttaaaatct gcatgatttg aaccgcaaaa ttcatatcta gattgttcaa aggttcatcc
     4021 agtaaaatat aatccgtgtc ttgcgctagc accattgcaa tatacgcacg ttggagttga
     4081 ccgcctgaaa gagtgtcaat cgattgatcc gctatttctt ctaacccaag gtgttccagt
     4141 gattcctgaa cgatttcctc gtcttttgtc gtcaattttc ctttcgtata aggaaatcgt
     4201 ccaaattcta ctaattcttt cacggtgatt tggacattta tttcattcga ttgtcgtagg
     4261 atcgataatt ttttggctaa ttcattggat ttccaagcct ttacttcgtt atggtctaaa
     4321 tagatttcac ctgtatcttt tggtatcaaa cgactgatca tcgacaacac cgttgacttt
     4381 ccagcaccat ttggaccgat aaaagcagtc aatttctttt caggaatcgt taactgtaca
     4441 tctttaacca ctgattttcc atggtacagt ttattgacct tttgtaaatc aatcatagga
     4501 tactcttcct ttcgcgaatc aattttgtga caaaataaat tcctccgaca aattcaacga
     4561 cgacactcac tgttgtattc cagccgaaaa tctgctctac tgcgagctgt cctcctgtca
     4621 gaaacagcat agctaccaga aagcttccga taaatagctg accatgacga taagtagaaa
     4681 agacacgata actgatattt gcgacaataa accctaaaaa agtaactgga cccacaagag
     4741 ccgttgatgt ccctgtcaaa agactgacac aacataaaat caaaaattca aaacgatctg
     4801 tgtctatccc gaggtttact gcttgttcat ttccgagatg gaaaacgtct agcacatgtc
     4861 gatatcgaaa taacaggtac cagcagccga taatgatcaa agaagcaggc aaaagatact
     4921 gactctggat attgccaaag cttgcaaata atctgccttg aagcaaatca tattcattcg
     4981 gatccatgat gacttgtaaa aaggtactga cactaccaaa aagcgtacct aaaatcatcc
     5041 ctgacatcag taataaaaaa agattcgctc tcattttttt caaaagaaag aaaaataaca
     5101 atagactcag cccgaccatc agcccaacgt tcaataagaa aaaaactgta cctgtctgct
     5161 gaagcatcgt cactcctccg ataaagaaga aaaggaccgt ttggacaagg acatataacg
     5221 actcaagtcc caatactgaa ggagtcaaaa actggttatg cgtaattgtc tgaaagctga
     5281 ttgtggcact gctgacagct agcgagacaa ggataaatgc aaaaattttc ttactcctca
     5341 atgcacatgc aaaatgccag tttccatatg tatggatcgt caagtacatt cctgtaacaa
     5401 ataaaacaag aacaagcata ccaataaaaa tagatggctt ttttttaagc atatcgtcgt
     5461 ccccttatca gaagataaag gaagatagcc ccaccgatga ttccaacgat cacactgact
     5521 ggtatttcat aaggaaaaat caacacgcga gcgacaagat cgcaaaatac taagaacaaa
     5581 ctacctgcta aagcagttac gccaattgtt ttgtgcactt gatctccata tattctagag
     5641 acaagattcg gaatgatcac tccgataaaa ggaatgcttc ctaccatgat caacatgact
     5701 gcactagcca atgatactaa gccaatgcct aaaaactgca tcttctggta atctattccc
     5761 agatttactg caaacgattc gcccagacca atgatcgtaa atcggtaagc aaatagccaa
     5821 accaagagca acaatggaac aacagcatac aataactcat aatttcctcg tatcacagtc
     5881 gcaaaattcc cttgaagcca tgaagtcata ttttgtacca actggaattg ataagcaaaa
     5941 aaagtagcaa ctgaaccaat gatattccct aacatgactc caatcaacgg aagcatcaat
     6001 tgatttttct ggaggaacaa tcgcgtaagg gataaaaaaa gcaatgttcc tgcaaaagca
     6061 aagcaaaatg ctgtcaagga tcggacaatc aaggatgcac caggtaggaa aatcatagcc
     6121 actaaaatac ctaaacgcgc actatccatc gtgcccgcag tcgttggtga aacaaattta
     6181 ttttgggtca aatgttgcat gatcaaccca caaatactac tggtcgctcc cacaaggatc
     6241 aaacaaatcg ttcgtggcaa acgagtcact agcaagattt gtaagctttg cccatctaac
     6301 tggagcagtt ctgacaattg tacattcgat actccgatga acaatgaaat actacttaaa
     6361 ataactaata atgctactag tcgtccgtag cgcatccaat ccacctttct aattgagaat
     6421 gattttcatt cttattttaa gtgaatcgtt gaacaaaagc aaccgctttt tcatatgttt
     6481 taggtattat tgggaatatg ctataatcgt aagaaagaca aagagaagaa ggagggtatt
     6541 ttttatggca caagatgatg taaaaagcca tttggataag ggactttatg gtacaccttt
     6601 agtgaaccct gaagaacagc ataaatattt agggacattc agggaacgct gctatcttag
     6661 tatgactgtt gctgaaatga tccaaccaaa aaacaaagaa aactttttaa aagaactcaa
     6721 aaaacatcca gatgcttcca tactcttgaa tggggctcta cccctttctg tccaaaacca
     6781 atatatccag ctagcaacga aaaacaatag ccgtttcact gtagtcaacg attacgtcac
     6841 tgcaaatcct gaggaattcg gattgctctt aacagcaaaa gaagcagtaa atgaacctgt
     6901 gattgatatc gaaaaaaaat acccagaaga aaactcttcg ccagctgaaa aagaaaaacc
     6961 aaaaaagaaa aaaagcttct gggatcgttt attttaaaac gaaaaaacac ataaattttt
     7021 acatgcaaaa aaagcttttg atcagattga catcaaatag gacaatctgt caaaagcttt
     7081 ttcttttaaa atacttcagg atataacaat ttcaatacat tcatcgtcaa tcttctgcct
     7141 tttcctgcaa aaggataaac atgcatatgc atggctggat tgaaatttgc ttcgatgatc
     7201 ccatatgctt ttttatcagt tgtaggatcg atttctttat ctggtataat caaatcgata
     7261 ccactaatct tagctcctag tgcttctacc gcagaaacgg cgattttttt gtaagcttca
     7321 gaaaattcat cagtcatatc gatcgaatca ccacctgtac tgatatttga gttctccctt
     7381 agataaacaa tttgattggc ttggggaact gattcgatcg tcaacccttg ttctttcaac
     7441 atcaattgtt ctaattcacc taattggatc aattctaaag gtgcacgatg attcgttcca
     7501 cgcaaagggt cactattttt ttcttcaaca agttctttca ctgttcggat actgtcacca
     7561 ataacattag cagggactcg caacatgatg gcttggactt ctccgtcgat cacaaagaaa
     7621 cgatattccg ttcccggcat aaattcttct actaaaaccg aactgtcttc acgaaaagca
     7681 atggctaaac ctgcttgata atcttccaga ctagctcctt ccttgaaaat cgtgatacct
     7741 aacccgtaat tggttgattt aggtttgatc acaaatgcct gctcggcaaa tctcggataa
     7801 gcttttaccg cttcttccat ggatgaaaat tctgcgcctc ctggtacccg gaacccagct
     7861 tcttttaaga cttttttcgt tactgttttg ttttccatga taagtgggac gatatagctg
     7921 tccttacttg tcatgtttgc atttttcaca tattctacat ggtcttggaa ttgcagacgc
     7981 aaaaattgat ccgactcatc taatactttg acttggacac ctttttggat ggcatcgaac
     8041 ataaatatct gagtagaaag ttccatttcg cgaaaaccgg ccaactgata gggacgttca
     8101 tggctttctt tataatattg atgtcccaat gccacagcaa cttcttgctg actcgatagc
     8161 tgtgcttctt gatagagttt cgctgctaac gtttgttcag gatgatccat ttgagtcaat
     8221 gcttcctcta ctaaataaag agaatccaac caatccaatt caaccagcat ttgcttcatt
     8281 ccttcaagaa tttctctacc ttcttgatga aattctgttt gatctgatgg ttgttctagt
     8341 gctactttgt tatttcgagt cgtaccttct gctacccatt ggtcacagtc ttctttttct
     8401 tctgtccata acatgtacat caggaataat tgtaagaacc gaacttgctc tggattgata
     8461 cccaatcttg cataaggatt caaatcgata ttccgcaatt caatataacg aacacctgtt
     8521 gttgctaaat ctgcgacttt ctttccgcct cggaaacgaa gcggtgtata aaattctttc
     8581 tcctccgaaa gtttcccttc ttcgatcatt cgctcgatat ctgctaaata ctgtttcata
     8641 gaagcatagg aaactttcac atttggatga ttcgtatagc ccagcgcact gttgcgaata
     8701 ctgcgaacag gttcttgcgg atgtgattca tctaaaaagt agttcttctc actcattggt
     8761 gaagcaccga ataaataagt gatcatccag cgatagcgca taaagtttct cgctgttttc
     8821 aaataaagtt ctgttttgaa ttcggaaaaa tcttgaaatt cttcctgatg gctgaacaat
     8881 gttcttagca actcatcgcc aaattcaaaa ttgaaatgaa taccgcttac catctgtttt
     8941 cgcttcccgt attcttttgc taaataacgt cgatacaaga catcttcgaa attttttaat
     9001 tttgcaataa tgatctcttc gtctttttcc ggtaaggcag gtggcatact taatggccag
     9061 atcatctctt ctttcggtat agaacgagca gtcacatcat aaacagcagc gagatactga
     9121 aataattcgg gaatagaatc ggtaacaggg gtgatcatct ctgtttgtgt ttcgctaaaa
     9181 tctgtttgga tataggggtg ataggatcga tcgccaaaga ttgctggatg atcggttttt
     9241 gctagatttc ctgcaagatc aacccgctgt ccttcccgtt ctataccaaa tcgagcttgg
     9301 tcgataaaag ggcgcgcatt gacatgcaat aataattgct taaaattcat cattaagtga
     9361 cctccaattc atatgaatct aattataatc aaaaagtcta agaaaaagaa cgaaattgtt
     9421 tgagaaccgc ttacaaataa gtataaaaat gaaggcatca aaaacacagc tatgttaaaa
     9481 acaatcattc aaacaaggag ctgcaacttt tgtcacagct cctgcttttt agacatgggt
     9541 tattcttctt ttagtaatcc tttgaacaat ccaacgacaa aatcatttgg atcaaacggc
     9601 tccaagtcat cgattccttc accaagaccg accaatttca ctggtaaatg caattcatta
     9661 cggatagcaa gtacgatacc gccctttgct gtaccatcta attttgtcaa aacaagaccg
     9721 gtcacatctg ttgtttcttt gaattgtttt gcttgaacca tcgcgttttg accagtggtt
     9781 gcatctaata caagaagtgt ttcttggggt gcttctggta attcccgctg gattactcgt
     9841 tttatttttt ctaattcgtt catcaagttc actttatttt gcagtcggcc tgcagtatca
     9901 actagtaaaa catctgcctg ctctgtcttt gctctttcca aagcatcgaa tacgacagct
     9961 gctggatcgc ctcctgcatt gccacgaaca acttcaacac cagcgcgttc gccccagaca
    10021 actaactggt cgattgctcc agcgcggaag gtatctgctg ctgccatcaa tactttttta
    10081 ccttcgtttt tgaactgatg ggcaagttta ccaatacttg ttgttttccc tacaccatta
    10141 acaccgacaa acaaaataac tgttagtccg tcttcttgga tatttagttc atttacttca
    10201 ttgattcctt ccgcttcata gagatccaca agtttctcga tgatcgcatt ttggacttct
    10261 gccgattttt tcgcatttct gagtttcact tcttgacgta atgcttcggt aattcgtatc
    10321 gctgtttcaa aacctacatc tgcaccgata agtgtttctt ctaattcttc aaaaaagtct
    10381 tcatcgactg ttcgaaaatt ggcaaacaac tcattcatcc gttcaccaaa tgttttacgc
    10441 gtcttttcta aacccttgtc gtacttctcc tgtacttcaa cttcttctgt tttctcttcg
    10501 ccagtaaaag cacgcttgat tttatcaaaa aatcccattt gactccccct actctaatac
    10561 aaatttttgg atcactttgg ctacgccatt ttcttcattg ctggctgtca cgaaatctgc
    10621 tgcttctttg acgaaaggca cagcattctc catcgctacg cctagtccag catattggat
    10681 catcggtaaa tcattttcct catcaccgat cgccatgatt tcttcttgtt tcaaaccaag
    10741 atcttttgcc aataaagaaa taccataagc ttttgtaaca ccttttggca taaattccaa
    10801 aagcatactt cttgttttga tgatctcata catttcatgg aattctgctg gtatttcttt
    10861 gattttagga tctaattctt cttggtcata agcgattact gccttattaa gcacaaattc
    10921 atctgtaata tcgcttatat ccgctggttg gaattgcagc agcttattca acacattgta
    10981 caaggattgc ttccttgggg cactaggcag ctgtaaaacc acattgtcag acaaaacatc
    11041 tagtggcaga tccaattgct gagccagttt gtataagcgt tggatatctg atacttccat
    11101 cactttcttt tccatgattt caccagtatc atttttttgt accaaaccgc cgttgaaagt
    11161 gatgctgtag tcaccttcat ctaccagtcc taattcttgc agataatgcg ccatcgcagc
    11221 taaggggcgt cctgtacata gaacgatctt gaccccttgc tgttttgctt gtgctaatgc
    11281 ttgtttattc tcatctgaaa tcagcttttg ttcatttaat aatgtaccgt ccaaatcgat
    11341 tgctactaat ttgatcattt tcgttcctac actttcgttt cgttgatttt cgcctcttca
    11401 ttgatatctt ccatccgtac agaaacgatt tttgatacac cggattcctg catcgtcact
    11461 ccgtacagta catctgctgc gaccatcgtt cctttgcggt gtgtgactac gataaattgt
    11521 gtgtcatctt ggaaatcgct caagtaacga ccaaaacgtt tcacgttggc ttcatctaac
    11581 gctgcttcta cctcatctaa gatacaaaac ggaacaggac agacacggat aatggaaaag
    11641 agcaacgcga tggctgtaag tgctcgttct cctccagaaa gcaaacttag actttgcagt
    11701 tttttgccag gaggttgtac ttcaatctca atacctgttt tcagcaaatc agttggttcc
    11761 gtcagtacta attcagcttt accgccgcca aacatatttg gaaagacaat tttgaattct
    11821 tgacggatag cttcaaatac ttctttgaat cttgtccgca cttcatcatc catctcatcc
    11881 atcgtttcaa acaattggtt tttcgctgct aacagatcat ctcgctgtgt tgctaagaat
    11941 gtatgtcttt cactgacttg ttcgtattgt tcgattgaat ttaagtttac tggtcccagt
    12001 ttttcaatct gttcttttaa ggatgaaacc tttgttctac tgtcttcgat atcggttgtt
    12061 tcttgatagt cttgggaagc tttctcaaat gtcagctgat attctgactg cagataggtc
    12121 aaatgattat ctaaaatcat ttcagctcgg tctttttgga cttctaattt ggattgttcc
    12181 gacagtcgtg ctttttgctc acgattacgc tctgccaaga ctgcttcaag cttatcgatc
    12241 tccttttgcc ttctatctcg ctgctctttg gcttttgcca actcggcttt taatgtttct
    12301 cgctgagcag ataattcgtt aatttgtttt tccaaacttt cttctgtgac ttcatgatca
    12361 gaaaagtctg ctgtcaatgt cgccaactgc ttttctatcg cttcttgacg ttcggttgcc
    12421 tctgcttttt ggactcgggc accgcggagc tggatctgca gatgattaaa ctgctctttc
    12481 aagacagcaa gatctgcttg ttcttgtgct ttttgacttt gtacttgtgc tcttctggct
    12541 tccatctggt cgctttcttg acttaacgat ttgatttctt cgtcgatttt ttgtcgttgc
    12601 gattcaagat ctgtttgttt gtctttcaat tcgttcgttt gcttttcgta atcttcgata
    12661 aactgctgca gttcacgtgt ctcaaaagtt gagatttgct tttctttttc aaaacgttca
    12721 agttcattta tgatgttttg tagctgattt gtcgcttctt gttcttcaaa acgtaattgt
    12781 tcgcctcgtg tacgcaaaac ttcttgttct tcggctaaac gagctgtttc ttgttgcagc
    12841 tcctgaactt ttttttcttg tgcttggagt tgtttatctg cttcttcaaa ttcactagtc
    12901 aactgtttca attcttgatt ttgtacaaac aaactgcctt ggtttcctcg tttattcgct
    12961 ccacctgtca tggatcctcc ggcattcatc acatcacctt ctaaggaaac cactcgatat
    13021 tgatagcgga tcgtttgcgc gattgcattg gcactagtta agtctttggc taaaaggatc
    13081 gttcctagca ggttttgaac cacagtttgg atttgatcgg gataactgac ttgttcactg
    13141 gcaatcccta taaacccttc tacagctgct gcttgtgtca atatatgagc cggtaattgt
    13201 cttggtttga ttgtcgtcag tggaaggaat gttgctcttc ctcctctttg ctgtttcaaa
    13261 tacgtgatgg cttgccgagc atctttttca ttttcaacga tgacatgctg ggctgcccca
    13321 cctaaagctg tttcgatagc taacgtgaaa tctgcaggaa catcgatcag ttcagccact
    13381 gctccgacaa tacccgaaag ctgctgttta tgctgcaaga ccaatcgaac cccttggtaa
    13441 aaaccaaaat agttttcttg gatttcttga aggcttttct tacgagcacg tagctgctgg
    13501 acttgattca ttaattgata cattttaggc tgttcttttg ctaatgcttc ttggaacttt
    13561 ttcccgtttt gttggatcat ttctaacttc tcttttgttt cagttaaaga tgattttagc
    13621 tgcgcctgct tttgtgtcaa ttcttctttt tgtaagatca atcggtctac tgatgcttct
    13681 acttccgatt gcttggctaa cgtctgcttg ctttttgctg tttcttgtat atattgacgt
    13741 tctagatact tcagttcgtt tcctacagct gcttgttctt gcatcaaatc aacatattga
    13801 tctcttaatt ctgccaataa ttctttcgtt gatttttggt atttttctaa ttcttgctgt
    13861 gtttttatga gatttgcttc agctttttgg atctctgttt ctttttctgc tgctgctttc
    13921 atcaagcttt cttgaagctt ttcaaaatgt ttaacctttt tttgtgcttc tgctaatgaa
    13981 gtttgatatt cttgcgagct tttttgtgta tgctttgtcc gctcctgcaa aacatctttt
    14041 tgtccttctg tctgcttcag tttttccgaa agatctaata atacttgctg atttttttcg
    14101 atcagacgat cggcttgcgc attttcttta cgctgcttcg ccaaaatgga ttcttgttct
    14161 tggatcgact cagatagttt gcctaattct agattgaatt tcgcaagctg tgcttgtttg
    14221 ttgtcccaat ctttttttgc tgtcttgatt tcagcaacca tcaaagagac atcggtttga
    14281 gtcagtgttt cttttagccg taaaaactcc tttgctgcct cactctgggc ggccaaagga
    14341 gttaactgtt cttccaattc gtggatgatg tcttggacgc ggctgaggtt gtcttctgtt
    14401 tcaaaaagct tctgttctgc tttctttttc cgctgcttgt atttcaagac accagctgct
    14461 tcttcaaaga tcccacgacg gtcttccggt ttactactga aaatcgcttc tacttttcct
    14521 tgagaaatga ttgagaacga ttcttttcct aatccagaat ctaagaacaa ttcttggata
    14581 tcttttaatc tgcagctttg tttattgatg aaaaactcgc tttccccagt ccgtcgataa
    14641 cggcgggtta cactgatctc attgtactcc aatggcagat aatgatcact attgtctaaa
    14701 atcactgtga cttctgcaat attcagctgc ttacgggtat cggaaccggc aaagatgata
    14761 tccggcattt ttccgcctcg cagactttta gcagattgtt ctcctaatac ccagcggata
    14821 gcctctgtaa tattgctttt tccgctgcca tttggtccaa ccactgctgt gacgctattt
    14881 tcaaaatcaa tgactgtctt gtctgcaaat gatttaaaac cggctatttc aattcgtttc
    14941 aaatacaaag ctttccctca cttactttta cagtgctgcc aatgcatttt ccgccgcagc
    15001 ttgttctgct aattttttcg atttcccttg tccagtgcca atcaattgat catctacata
    15061 aacttcgatc caaaatactc gttcatgagc tggaccttct tcattgatca atcggtattc
    15121 gattgataca tcacctgatt tttgcaagac ttcttgtagt tttgttttat gatccatctc
    15181 atgtgaaaaa gcacctgctt tgactttagg aaaaatcact ttttcgatga atgcatgggt
    15241 cgtgtcgaat ccctggtcaa gatataacgc gcctaaaaaa gcttcgaata ggtcgcaaag
    15301 caatgctggt cgcgtacgtc ctcctgaatt ctcttctccc ttgcccaaca aaacatattg
    15361 atcaaagtga cattctttgg caaatttcga caagctgtct tcgcgaacga tcgcagctcg
    15421 tgtttttgtc aattttcctt ctggcaaatg aggatataaa cggaataaat actgagaaac
    15481 catcagttct aaaacggcat ctcctaaaaa ttcaagtcgc tcattatccg ataattgcag
    15541 attgcgatgc tcattcacat aggatgaatg ggtaaaagct tgttcaagca aatttagatc
    15601 gtgaaaaacg atgttatagt tttcttttag ttcttttgtc agctggttgt ccattttcca
    15661 cattcctttt atggatattt tttcgatact tccttattat actggttttt tgtcaaattt
    15721 gaaatggttc ggtcaaaagg taagtaaagc tttaataatc ctagtgattg acgagaagtt
    15781 gtgaaaaatt cgtttagttt cgagtatgaa gaaaaaattt tgtgaaaatg gctttccata
    15841 tttttgcata aatttgactt aataccgaga aactggactt tgaacaccgt ttattggaga
    15901 agtcatgaaa tcagcgcctt gacctgaata ttagagaaat aaacagaaaa atatcaaaaa
    15961 accgaaaaag gacatcgccc tttttcggtc atttttagtg attatgaacg tgaatctgct
    16021 gtaacagaaa catttgccca tgggtttggt acatcataag accaatccca gtctttgaca
    16081 cggtcgttga ctggcaatac ttcattacgg aacaacgttg gaatcgcaaa tgcttcttca
    16141 aatgcatatt gttgccattt ttgatatgct tctttttggt actcttcatc aaatgctttg
    16201 cttgaactga tatcgttgat caattttgta ttttcttctg attcataacg agtgtagttg
    16261 aacgcagcat ttggtcccca tagtccactt ggatttggat cagttcctgt attccaagca
    16321 gcttggtaaa catcaatttc aggatcatcg ttttctaatt tatcatagaa agaattgaat
    16381 tcgatcagac gacctgttgt gtattggaca tccaaaccaa tttctttcca ttgttggata
    16441 tagtaatctg ccaatggttg cgctgtttcc ccaccggaca tcgaagcaaa gttgattgtt
    16501 agtttatttc catctttgtc ttctcttaaa ccatcgtcat ccacatcttt gtagccagca
    16561 tcatctaata gttttttcgc tttgtctaga tcttgtgtga atcctttcat ctcatcatca
    16621 tggtatgact ggaatactgg aggaatcaat gaagtcgcat tgcttctcaa acctgcatag
    16681 aaacgttcac ctactgcatt attatctaat gcatagccca ttgcttgacg taatgattta
    16741 tcagccattt ttgaatctgg attgtattcc acactctttg tatctgcatt ccatttacct
    16801 aacttgaatc ctagataagt ataagaagtt tgttcacggc ctaagtttgt atagccagca
    16861 atttctttat aggtatcgta agtatctgtt ggcatttgga aaatcatatc atataattta
    16921 gagttcaatg cttcagttgc acttgccatc ggtacagatt tcatcgtgat ctttttcaat
    16981 ttaggcgtac cattccagta gtgctcgttt ggcaagtatt ctactgattc tcctgcaacg
    17041 atattactca tgtaataagg gccaaaagtc actggatttt tacgtacttg atcgcttgat
    17101 tccatatctt tgattgctac accttctaac gtatgttttg gtgctgcata tgaccagata
    17161 ccaccacccg attgaagcat gcttgggttt acttctttat attcgatttc aactgttttg
    17221 tcgtctattt ttttgattcc ggagattgtg tcggcacttc catcatgata ttcttccata
    17281 cctacgatgt tacggaaagt gtcgtcataa cggataccag tgtaatcttt attcccaatg
    17341 atttcgtaag ggaaaatcac atcgtccgcc gttacatctt caccatctga ccatttgacg
    17401 ttgtctttta ctttgattgt cgcttttttc gtatcttcat ctagatcaag cgttgcagct
    17461 ccgtcgtctg tgattctgaa atcgtcatcg tttgcaaata gcggttcatg agaaggagcc
    17521 ataaaagctg catcatatga atcttggtaa aactcccact ggaacaatcc tttaaactga
    17581 ctgtccgttg cgaccgcagc ttctaattcg ccgtcttcga ttgcatcatc attatttttt
    17641 gttgcaattg gaaaagttaa ttcctctttg ttattgccgt tgcctccaga gttgtttcca
    17701 gaggtatctg tacctccgcc cccgcatgct gcaagaacga cagcagacat cagcgctgca
    17761 aatccaaaaa tcgttttctt cattcctatt tcccccttcg cttctatact agcctaaccg
    17821 ttggcgcgca tctgcagaac gcttgaatgc ttgtccaaca taattgatac tcaacatcaa
    17881 aactaaaata aggattgatg cgggtaacca gatccattgc cgattcacta agacttctcc
    17941 gttacttgca taaccgatca atgtacctaa actaggtaca tttgccggca agccgaaacc
    18001 taagaaagtc aaagttgtct cgataccgat gtttgccgca aagttcatcg tcaaattagt
    18061 aatgattaat gaacttagat tcggcatgat ttcgcggaac atgattttaa aatcattggt
    18121 tcccattgtt tttgaggcac tgacataatc tcttcttgct tcagataatg tcttgcttcg
    18181 gaataatcgg gctttagcta cccaataaaa tgcactcatg atgaatataa aggaccagac
    18241 attgtatctg gaaataatcg atacgaagac gataatgatc agcatgatcg gtagaatcat
    18301 gataaaatca acgatccgca tcaagatgtt gtcaatgatc cctccgtagt agccagagat
    18361 gatccctaat ccaaccccga tgatcgaagt aaggatcgtg atcgcaaatc cgatcgtaac
    18421 agagtttcta gcacctatga tcaattgtcc taatacatca cgtccgcctt catctgctcc
    18481 cagaatgaac ttttgtcctt gtgtcgaaac tgttcctggt gcagcgtatt tgtcgaagat
    18541 actaacatac atcactttgt cttgatcggt cagcatcgca ccgataaaga tcactaataa
    18601 taaaaccact agcaaaccta atgaaaaaat agcaatttta tcttttttaa attctcgagc
    18661 aatcattcgt atccccatag gaggaatact ttcttgaact gctgtattgt ttttttcact
    18721 catcgatcca ttctcctttc tgattggttt tattgaatcc gaatacgcgg gtcaacgata
    18781 ctcatgatga tatctgaaag taatgtcccc actagcgtcg cgattcctaa aatcaatact
    18841 aaggatgtga tcacggtata gtcccgttgg ccgatggaat caataaatag ttttccgact
    18901 cccggataac caaaaatctt ttcgataact acagaaccag caatcaacga agtgatttca
    18961 taccccattt gagaagccat cggcaacgct gcatttcgga aaatatgtcg actgaatact
    19021 ttattcgtcg gtaccccttt ggaacgggct gttctgacaa aatcgagcga tttcgaatcg
    19081 attacttcac ttcgcagata ttggattgtg acggcagtcg ccaaaaatgc ttgggtaatt
    19141 gatggtaaca gaatatattt caaacggtct atccattgag tgataaatcc accttgcaaa
    19201 cctagtgttt gtgatccact tgttgggaac cagcctaaac gataaccaaa aacaaatagc
    19261 atgatcaagc cgaagataaa taaaggaaca gcaaaactaa agaagttgta gattacgaca
    19321 atcttatcca agattgaatt ttgataccgg ccagcaagca tccccagagg cacagcaatc
    19381 aaataagtaa ggacaactgt caagatcgat agatataaag tgttgatgat ccgttcgccg
    19441 atcaagtcag caacagagcg tttgtataag aaactttgac caaaatcgcc ttgcaaagca
    19501 tttccgatcc aacggaagta ctggacatac catgggtcat tcaagccagc tgcttgtcgc
    19561 atctgttcga tcactgccgg atcctgattt ggattgatca aaccggtaaa tggatccccc
    19621 ggcatcgctt gggctaataa gaagatcaga acacttaaaa taataacttg cgggatcatc
    19681 aataaaacac gtcgcagaat cgttttccac attagcttgc acctccatct ttcaacgcca
    19741 ctttgtgtgt agttgtgatc gtacgcaaat cgtagactcg accagtcgca tcatagtatt
    19801 ctttttggtt ttggatatac tcttgttcta ctcttcgtcg attttctttg tggctttccc
    19861 tattttctac atcgatttgc ggaatagctg aaagcagacg tttcgtatag atatgtcgcg
    19921 ggtcattata aatgtcttct cttgtaccga tttctacaaa tcgacctttg tacataattg
    19981 cgatattgtc gcacatgtgt tttactacac ctaaatcatg tgaaatgaat aaatagctga
    20041 ggccgtattc ttgctggatt cgtttcatga aattcaatac ttgcgcttga acagatagat
    20101 ccagagcaga tacaggttca tcggctacga tcagttttgg attggtagca acagcacggg
    20161 ctactcctag acgttgacgt tgtccgcctg aaaattcatg aggatattta tatagcgcat
    20221 cttccggcat cccaacgata tctagcagtc cttttacctt tttcttttct tcttgatcgc
    20281 ttaaccgttc aaaatttcgg atgggttcag caatgatatc caacactcgc tttttaggat
    20341 tcaaactgga cattgaatct tggaagatca tctgcacatc cttgttataa cccatttttt
    20401 tgcggttgct tcgcttcgtt acatcttttc cttcatacat gatttgtccg gctgttacct
    20461 tttctaatcc cacaacggct tttcccgtag tagattttcc agagccagac tctcccacta
    20521 aaccatatgt ctttcctttt tcgataataa aatccacacc atctacagca tatacatgat
    20581 ccgttactcg attgaagaat ccactgcgaa tagggtaatg aactttcagg tctttgattg
    20641 tgattaattc tgtcatctac acctctcctt tttcgtctct aaagtggaaa tgcttatagc
    20701 aggagcaacg taccaagtgg ttcggtgcga cttcatgaag gataggctct tcttcatgat
    20761 cgcttgctgg tatccatgga atacgtggtg caaaccgaca acctttacga ggcatgtttt
    20821 tcagagaagg aacgacacct tcgatcacat ggagttctgc atcatgattt ccttcttgtg
    20881 gtatagagtt cagtaaagag cgtgtataag gatgcttagg atgatcaaac agttctttta
    20941 cacctgctac ttcaacgaac tgtccgccat acatcactgc cactcgatca gccatttctg
    21001 ctacgactcc taagtcatga gtgatcaaga tgatccctgt ttttgtttct tcctgaaggt
    21061 ctttcagtaa atcaagaatc tgtgcttgga tggttacatc tagggctgtc gtcggttcat
    21121 ctgcaatgac gattggaggt ttgcaggcga ttgcgattgc gatgatgaca cgttgtctca
    21181 taccgcctga tagttcatgg ggatattggc gtccaactct cgcaggatta ggaatcccta
    21241 cttgttctag caattctagt gcacgagctt gacgctgttc tgcattcatc tttgtgtgat
    21301 aggaaagtcc ttctttgatc tggtcttcga ttctcatgag aggatttaat gcagacaacg
    21361 gatcttggaa aatcattccg atatcatttc cgcggatttt attatagagc gtttcgttca
    21421 ggtcgattaa attcagatcg ttatataaaa tctcacctgt aatttttgtg ttattaggat
    21481 caagtaatcc cataatcgta gtagccaagg tacttttccc gcacccagat tccccaacga
    21541 ttgctaaaat ctcgtttttg tctaaagtaa atgaaacgtc atctactgca tcgtaatagt
    21601 catctttgag tcggaatcct gtatgtaggt tctgaacttc taataattgg ttatcggaca
    21661 atcgaatacc cctctctgct tcctaaatgg aagtttttct taataaaaaa ggacattaca
    21721 cagaataaat cgttttataa caaaggttat tcttatcttt gtgtaatgaa ttattaataa
    21781 ttatatagaa aaccgcttta ctttgcaaca taatttctgt ttatcgccag aattatcaga
    21841 aaaatgactt ttacgtgtca aaaagattaa a
//
LOCUS       DARBET010000043        21505 bp    DNA     linear   BCT 07-NOV-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4027
            SAMEA114569841-rid20112183.denovo.043, whole genome shotgun
            sequence.
ACCESSION   DARBET010000043 DARBET010000000
VERSION     DARBET010000043.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA114569841
            Sequence Read Archive: ERR12203666
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 21505)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 21505)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001972618.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001972618.1
            Genome Coverage       :: 132x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/07/2023 13:18:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,041
            CDSs (total)                      :: 2,975
            Genes (coding)                    :: 2,908
            CDSs (with protein)               :: 2,908
            Genes (RNA)                       :: 66
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 67
            CDSs (without protein)            :: 67
            Pseudo Genes (ambiguous residues) :: 0 of 67
            Pseudo Genes (frameshifted)       :: 26 of 67
            Pseudo Genes (incomplete)         :: 28 of 67
            Pseudo Genes (internal stop)      :: 20 of 67
            Pseudo Genes (multiple problems)  :: 6 of 67
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..21505
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA114569841-rid20112183.denovo.043"
                     /isolate="VRE4027"
                     /db_xref="taxon:1352"
                     /country="Denmark"
     gene            186..887
                     /locus_tag="SCJ86_001766"
     CDS             186..887
                     /locus_tag="SCJ86_001766"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400355.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Crp/Fnr family transcriptional regulator"
                     /protein_id="HEG1169307.1"
                     /translation="MRVNKIDFHFSKLRYQPDFEHFSDEEFQQIKEHTVLRSYKKGQI
                     LFDDGDPRQRLYILTKGLVRLERYDESATYYYYDYVNTNNLFPMSGMFGEETYTYTAQ
                     AMTDIETFYLPVSYYEKLARKNPEQLIYMIQKISKVVEIHELRIQIGLTSSAFDRVKQ
                     NLFILKEELGVPTKDGHITIPYPITLKELAVNSGTTRETAGQVIKQLRDSGLLDYDKK
                     RFTFCKTATTPAMRE"
     gene            complement(1496..2512)
                     /gene="tsaD"
                     /locus_tag="SCJ86_001767"
     CDS             complement(1496..2512)
                     /gene="tsaD"
                     /locus_tag="SCJ86_001767"
                     /EC_number="2.3.1.234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317795.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     transferase subunit TsaD"
                     /protein_id="HEG1169308.1"
                     /translation="MNEELILAIESSCDETSVAVVRNGTEILSNIVASQINSHKRFGG
                     VVPEVASRHHVEQITLCLEDALVEAGVSAEDLSAVAVTYGPGLVGSLLIGISAAKAFA
                     WAHQLPLIPVNHMAGHIYAARLVKPFQFPLMALLVSGGHTELVYMQEDGSYEIIGETR
                     DDAAGEAYDKVGRVLGLSYPSGKEIDQLAHQGKDNYHFPRAMIHEDNYDFSFSGLKSA
                     FINLVHNAQQRGEDLDKNDLAASFQASVIDVLITKTLRACQNYPVKQLIIAGGVAANQ
                     GLREGLQDALAAKLPEVELVIPPLRLCGDNAAMIGAAAHVEMQKRNFASYQLNADPSL
                     VLSE"
     gene            complement(2509..2973)
                     /gene="rimI"
                     /locus_tag="SCJ86_001768"
     CDS             complement(2509..2973)
                     /gene="rimI"
                     /locus_tag="SCJ86_001768"
                     /EC_number="2.3.1.266"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335773.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosomal protein S18-alanine
                     N-acetyltransferase"
                     /protein_id="HEG1169309.1"
                     /translation="MICRKKDFDEAYLAECVWMISENAYPHGSPWTKMQFLADIQQPH
                     SDYLLLAEDNQICGFIGYSKVIDEVEITNIAVAVSEQGKGHARHLLQLLITEQIKDSL
                     HVFLEVRLSNEAARKLYESEKFRILGKRKSYYRNPTEDAIIMSRKLKTETMK"
     gene            complement(2980..3522)
                     /gene="rimI"
                     /locus_tag="SCJ86_001769"
     CDS             complement(2980..3522)
                     /gene="rimI"
                     /locus_tag="SCJ86_001769"
                     /EC_number="2.3.1.266"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348676.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosomal protein S18-alanine
                     N-acetyltransferase"
                     /protein_id="HEG1169310.1"
                     /translation="MLKKFRAYARSFFQDKMIYTEKTVEQIDRRYVLRELTTEDIKEL
                     LDVEREVYNGELPWTKSAFISELKSPLPHLYLGIFDNGRMIGFIGSRAFGLDCHITNI
                     AVRPAYQRKRIGSLLIDEIENFAIMNRCETMSLEVRMSNQDAQRLYRKLGFQATTIKK
                     RYYTETKEDALDMIKLLENE"
     gene            complement(3506..4228)
                     /gene="tsaB"
                     /locus_tag="SCJ86_001770"
     CDS             complement(3506..4228)
                     /gene="tsaB"
                     /locus_tag="SCJ86_001770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326913.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     dimerization subunit type 1 TsaB"
                     /protein_id="HEG1169311.1"
                     /translation="MITLGIDTANQTLAIGVVEDEQILGQIQTNIKRNHSVTLMPAID
                     QLFADLQISPKDIDRIAVSDGPGSYTGLRIGVTTAKTIAYTLDKELVGVSSLKTIAAN
                     CIGVDNIIVPMFDARRKNVYAGAYRWKENRLETVLEDVHISASDLFTQLQKLNEAIYF
                     VGSDCHKFFSEIKEILPSAMINTVPAWDIPSGVTVAQLGALVEPVAEKQAFLPRYLKR
                     VEAEEKWLETHTPGDESYVEKI"
     gene            complement(4419..5399)
                     /locus_tag="SCJ86_001771"
     CDS             complement(4419..5399)
                     /locus_tag="SCJ86_001771"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301320.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HEG1169312.1"
                     /translation="MATIKDIAQLAGVSPATVSRVLNYDPDLSVGLATKQKIFEAAER
                     LNYTKHRKNAANTKQTLRLIQWYDDTEELEDLYYLSIRLGIEKKAEEANVTLLKETWD
                     SISDLPADGTIALGKFDEAQLLEMKGSQNALLLVDSDGTSQGIDSLVVDFKSSVQKVI
                     EHFVSHKAQHIAMLAGTEYTKKNHQRLKDPRIMAFQQVLAEKGYDEGPIIEADFSVES
                     GYQAVKQYLKTNPHVPDALFAANDALAIGALKAIQEKGLVVPEDISVMGFNDVSVAKY
                     VTPSLSTVKVYTEWMGELAVETILQLSNSKAPVSRKIIVETELIIRESTK"
     gene            complement(5423..6907)
                     /gene="galT"
                     /locus_tag="SCJ86_001772"
     CDS             complement(5423..6907)
                     /gene="galT"
                     /locus_tag="SCJ86_001772"
                     /EC_number="2.7.7.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342154.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-glucose--hexose-1-phosphate
                     uridylyltransferase"
                     /protein_id="HEG1169313.1"
                     /translation="MSISQTICDFATLAIEAGGWMEMDRLYLQNRLLGMVGEDALESI
                     EVRPVKEESVDLLDQLVAKARENGVLSELPAEQEIFEAQLMDFLTPPPSVVNAFFAQH
                     YSKSPEEATEYFFNLSKKNDYIKTRAIAKNIKFPAESEYGELEITINLSKPEKDPKQI
                     AAEREAVKVDYPTCMLCMENEGYRGRLNYPARTNHRIIRMNLDGESWGFQYSPYAYYN
                     EHSIILSEQHRPMKITKETFARLLRITEVLPHYFVGSNADLPIVGGSILSHDHYQAGR
                     HEFPMAKAPIEKLIKLEDYPQVIAGVVKWPMSVIRLQSTEKNLLIEAANSVLEKWKAY
                     SDETVSIRAFSADGTPHHTITPIARRRDNYFELDLVLRDNNVSEEHPDGIFHPHKDVQ
                     HIKKENIGLIEVMGLAVLPPRLKPELVEVEHFLLGEENQMAEYHRPWAEQLKKEHPDL
                     TKDTVTDVVQKSVGQVFARVLEDAGVFKRDEKGQAAFERFTAIL"
     gene            complement(6919..7908)
                     /gene="galE"
                     /locus_tag="SCJ86_001773"
     CDS             complement(6919..7908)
                     /gene="galE"
                     /locus_tag="SCJ86_001773"
                     /EC_number="5.1.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288452.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-glucose 4-epimerase GalE"
                     /protein_id="HEG1169314.1"
                     /translation="MTILVLGGAGYIGSHAVDQLVQKGYQVAVVDNLLTGHKQAVHPD
                     AHFYEGDIRDKEFLRSVFEKEPIEGVIHFAASSLVGESVEKPLMYFNNNVYGMQILLE
                     VMHEFNVNKIVFSSTAATYGEPKESPITEDTPANPKNPYGESKLMMEKMMKWCDQAYG
                     MRYVALRYFNVAGAKADASIGEDHTPETHLVPIILQVALGQRKALAVYGDDYDTPDGT
                     CIRDYVQVEDLIAAHILALEYLKEGNESNFFNLGSSKGYSVKEMLEAAREVTGKEIPA
                     EIAPRRAGDPSRLVASSEKAREILGWKPEYTDIKAIIKTAWDWHVSHPNGYEE"
     gene            8156..8323
                     /locus_tag="SCJ86_001774"
     CDS             8156..8323
                     /locus_tag="SCJ86_001774"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735150.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3042 family protein"
                     /protein_id="HEG1169315.1"
                     /translation="MKKFVSGILVGSLATAAAVAGLVASVKKTVIDPIDEKEAMIEEN
                     RKKAMRKRVSR"
     gene            complement(8385..10196)
                     /locus_tag="SCJ86_001775"
     CDS             complement(8385..10196)
                     /locus_tag="SCJ86_001775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338390.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD/NAD(P)-binding protein"
                     /protein_id="HEG1169316.1"
                     /translation="MKIGIIGAGPRGLSVAERILHNNQDRHSLELFLFDPNGPGGRVW
                     RLNQPPELLMNSVSQQVTLFTDETLSSGGLVTQGPNLFQWSKGPATDYIEQTVTKNKQ
                     LIIEEINRLEKDQQSTRCLYGLYQRWFFSKLQEEFPASIQFVYSLVKKVSKRKDGYLL
                     QTDQQSYSIDQLVLASGHWENDETEEERVWVDYAKKNVLLYQLPSNPADVDLSKIPPK
                     ENVFLRGLGLSFFDYVGLFTVSRGGRFENEKGKLVYHPSGKEPIVYSGSRRGLPYYPR
                     GRNQKQGGAMAWPRLLTKENLEQWHQSGFLTGETFFDYLKKDVELFYYKKIIEEYHLP
                     IPKKNFEHDFLSLSSEECLGKHPALIPYRWDWSYLETPLAYQNEPFSEASRIFIEGQI
                     KEAEKGNCTGAFASTFDALKDWRDPIRQAIEWHVFSAKEYKEILWGWFTHLNSFLTIG
                     PPIIRTKELAALIDAGIFHLIEPPFDIQQKHNRYVISLQDKEISSRYLIEARLPQTSF
                     SRSSNPVIKNLKENKLVRPFSFSDNTGSYHSEAIDVERGTNRVIDASGEIQSDFYCYG
                     IPVEGVDWLTAAVARPYTDPWNLRQADKIAQLILTDK"
     gene            complement(10193..10558)
                     /locus_tag="SCJ86_001776"
     CDS             complement(10193..10558)
                     /locus_tag="SCJ86_001776"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288459.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF488 family protein"
                     /protein_id="HEG1169317.1"
                     /translation="MIKLKRVYLDAEKEDGYRILVDRFWPRGISKEKAQLDLWLKEIA
                     PSNEARKTFDHVPERFASFAKKYQQELIFEEEKKQAIFKLSQLMKEHPVITFVYGAKN
                     EHQNHAVLLKKFIEMEACK"
     gene            complement(10721..11116)
                     /locus_tag="SCJ86_001777"
     CDS             complement(10721..11116)
                     /locus_tag="SCJ86_001777"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288461.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhodanese-like domain-containing protein"
                     /protein_id="HEG1169318.1"
                     /translation="MVLWVINIILLLIVLAIILWEVYLRVMAKRSATTLTEEEFREGM
                     RKAQVIDVREKDVFDAGHILGARNIPYTVLKDSLGSIRKDQPVYIYDQKKSLSIRTAN
                     KLRKAGYQEIYLLKGGYDGWSGKIKSKKV"
     gene            complement(11134..12096)
                     /locus_tag="SCJ86_001778"
     CDS             complement(11134..12096)
                     /locus_tag="SCJ86_001778"
                     /EC_number="2.7.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005879491.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family glucokinase"
                     /protein_id="HEG1169319.1"
                     /translation="MDKKIIGIDLGGTTAKFAILTPEGEIQQKWSIDTNILDDGKHIV
                     PEIIESINHRLNLYNMKAEDFIGIGMGTPGSVDSEAGTVIGAYNLNWTEVQFVKKLIE
                     AGTGIKFAIDNDANVAALGERWKGAGENDPDVVFVTLGTGVGGGIVAGGNLIHGVSGA
                     GGEIGHITVDPEGFECTCGKRGCLETVSSATGVVRLGRFLAEEYAGDSKLKAMLDNGE
                     EVTSKDIFEMAQEDDPFALMVVDRVCFYLGLACGNLGNTLNPSSIVLGGGVSAAGEFL
                     RSRVEKYFKEFTFPQVRESTKIKLAELGNEAGVIGAASLALKFA"
     gene            complement(12096..12308)
                     /locus_tag="SCJ86_001779"
     CDS             complement(12096..12308)
                     /locus_tag="SCJ86_001779"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YqgQ family protein"
                     /protein_id="HEG1169320.1"
                     /translation="MESLYDVQQLFKKFGIFIYVGARIYDIELMTIELRNLYDSRLID
                     HDTYMTAWRILKREHRIEESRKKGNL"
     gene            complement(12329..13027)
                     /locus_tag="SCJ86_001780"
     CDS             complement(12329..13027)
                     /locus_tag="SCJ86_001780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhomboid family intramembrane serine protease"
                     /protein_id="HEG1169321.1"
                     /translation="MNYQQQIKLRAWLRRPLWTYAFLGIQTAVFLMMELFPRLEIPYY
                     AGMYGPYLVHFNEWWRLVTPIFIHFGLMHFVMNSLILYFMGQQIEAIYGHWRFFLIYM
                     FSGIMGNTASFAFNEANVLSGGASTSIFGLFGALFILGFHFKHNPAIQQLVRHFLLFI
                     VLTFVFGLLDTSVDVWGHVGGLIGGLVLGNVLGLPKQQTSYSIHQRILSTLVFVFLFV
                     ICILLGLKKYGLLV"
     gene            complement(13047..13589)
                     /locus_tag="SCJ86_001781"
     CDS             complement(13047..13589)
                     /locus_tag="SCJ86_001781"
                     /EC_number="6.3.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302613.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-formyltetrahydrofolate cyclo-ligase"
                     /protein_id="HEG1169322.1"
                     /translation="MDQLKVRLRRKGIEKLKNLAKSGKKKEKEQRIYKQLFASEYWKS
                     AKVIGTTLSNEFELDTQPVIQRAEKEQKKIVVPKTLPKRQMAFYEMDEQSVLVRSSFG
                     VLEPQSEKLYLPEMIDLLIVPGIIYNRAGYRIGFGGGYYDRYLNNFHKKTCSLVFREQ
                     LNEQWQPESFDQKIQQLFID"
     gene            complement(13721..14725)
                     /locus_tag="SCJ86_001782"
     CDS             complement(13721..14725)
                     /locus_tag="SCJ86_001782"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343565.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron ABC transporter permease"
                     /protein_id="HEG1169323.1"
                     /translation="MKKIYWTFYFLLFVLVILSIGSLMIGTPFIYLDQLFATFIGKES
                     SSLALIVREFRVPRLLISLLAGACLGVSGYILQGVTRNELADSSILGINAGAGFLVML
                     YLGFFSHYSLPFLLLVVAFLGGVLAACCVYLAAYDRNGFLGMNRILLSGIAVNAGLSA
                     LTLLCTIQLSKENYGFVNAWLAGSIWGASWPYVWALLPWAVILIPLGGLYAQRIVLLS
                     FGQERAQGLGLNVAKSQKILLLLAVALASGSVAVTGSLSFVGLLAPHAAKLVVRKENN
                     WTFVLSGLFGSIFLLSADMVARVILPSGEVPTGILIALFGAPYFLYALFIRQKHALNS
                     "
     gene            complement(14722..15711)
                     /locus_tag="SCJ86_001783"
     CDS             complement(14722..15711)
                     /locus_tag="SCJ86_001783"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron ABC transporter permease"
                     /protein_id="HEG1169324.1"
                     /translation="MRTMKKNRLLLLVCFVLLIIFFVALSNGAATVEKADFWQALFDF
                     DRSKQSHQIIRNIRLPRVAGAFLVGSCFALSGALMQGVTRNPLADSGLLGINAGASLA
                     MALSFAFWPKASDFSVFLMSLAGSFAVTLLVFGFLQFSKMGMNPLQLILAGVAISSFF
                     TAISQALTQFFNLQQDLAFWFVGGAANITWKQLAVLFPLYILAVLVSFSLGKAISLIH
                     LSETHALALGSHPKRTRLIVFCLVAVLAAIAVSLVGPVSFIGLLVPPVVRGMVGTDYR
                     YVLPVSFLVGGGLVMLADFTARMINPPFETPFGLVIALIGVPFLLIQVRREQR"
     gene            complement(15708..16514)
                     /locus_tag="SCJ86_001784"
     CDS             complement(15708..16514)
                     /locus_tag="SCJ86_001784"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345987.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEG1169325.1"
                     /translation="MTRLRAADIAAGYENKKIIENMSVTIPEGKITSLIGPNGSGKST
                     LLKSFTRILPVKSGKIIVNNSDISTYSSKELAKKIALLAQTSEHPTGMTVEEVVSYGR
                     YPYQKLFSGMDTQDHEAIEWALDATGLAGFRMRDIATLSGGQTQRVWIAMALAQETDI
                     LILDEPTTFLDPAHQLEILQLLTEINQMKQTTILMSIHDINHASRFSDYLLAMKNGKL
                     ITEGTPEEVITKECMKKIFEIDATIVTLPRTNKPVFLTYDLLPKEQEDQL"
     gene            16680..17636
                     /locus_tag="SCJ86_001785"
     CDS             16680..17636
                     /locus_tag="SCJ86_001785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005874146.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HEG1169326.1"
                     /translation="MKLKTKVFTLSSILLLAGVLSACSPSSDKETATTANSTTSSNST
                     HTVTDTLGHKVEIPNAPKRIIGSYLEDYLIALGEKPIAQWTVGSGSIQDYLQDDLKDV
                     PTISYDLPYEKVLSFEPDLLLISSSATVEGGKYEQYSKIAPTYVVKNGTDVTWEEQLK
                     DVGKALAKEKEAEKIISDYQKSIKKTREELADKIENKTAAVLWVTNNSAFMVSKNRSS
                     GRIVYDDLEFGVPDLVEEVSKKATSDWSAVSSEKLAELDADYIILVNSDKDAAMFNEP
                     SWKNLKAVTDNHVLQFGPESSWLYNGPIASQEMVNDIKEKLN"
     gene            complement(17713..18231)
                     /locus_tag="SCJ86_001786"
     CDS             complement(17713..18231)
                     /locus_tag="SCJ86_001786"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306410.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrofolate reductase"
                     /protein_id="HEG1169327.1"
                     /translation="MFISMWAQDKNGLIGKNGLLPWRLPNDMRFFREHTMDRILVMGR
                     KTYEGMGDLSLPYRHIIVLTTQPNFRTKENAEVMHSIDELLSYSQTISEDIYVSGGSR
                     IFQELLPYTGRIWRTLIDSTFEGDTYIGNIDFSDFALIEEYEGITDQENLYAHRFQKW
                     ERVKNRVASKEE"
     gene            complement(18319..18468)
                     /gene="rpmG"
                     /locus_tag="SCJ86_001787"
     CDS             complement(18319..18468)
                     /gene="rpmG"
                     /locus_tag="SCJ86_001787"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016172256.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L33"
                     /protein_id="HEG1169328.1"
                     /translation="MRVNITLECTSCKERNYLTSKNKRNNPDRLEKQKYCPRERKVTL
                     HRETK"
     gene            18696..19142
                     /locus_tag="SCJ86_001788"
     CDS             18696..19142
                     /locus_tag="SCJ86_001788"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002371264.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2188 domain-containing protein"
                     /protein_id="HEG1169329.1"
                     /translation="MPWNMKDFPASMKNLDKLTRKKAIDIANALLDEGYPDNRAIPIA
                     IDQAKKWYEDASESEKRTFEKEKNPSKTDEHDTNPRAGKLLDSDVIVEYEEEQWIVKS
                     KGAKKASNHFDTKKEAIEKGKQVAQNKESTLFIYKKDGTKEKEIDY"
     gene            complement(19337..21232)
                     /gene="asnB"
                     /locus_tag="SCJ86_001789"
     CDS             complement(19337..21232)
                     /gene="asnB"
                     /locus_tag="SCJ86_001789"
                     /EC_number="6.3.5.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016176439.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="asparagine synthase (glutamine-hydrolyzing)"
                     /protein_id="HEG1169330.1"
                     /translation="MCGIVGFVNDKDNKKTIINTMMDRIVHRGPNSSGEYIDKHVALG
                     FRRLSIIDLEGGTQPIYNEDRTKIIIFNGEIYNYQPLREELIAAGHVFQTHADTEVLL
                     HGYEEWGTELLQKIRGMFAFAIWDNEKNELFGARDHFGIKPYYYAEMNGTFMFGSEIK
                     SFLPHPDFNKELNKEALKPYMTFQYSPLNGETFFKDVYRLPEGHYYTYKDGKIDIQQY
                     WDADFETKETHSRQEWIEKIDETVQASIEAHTVSDVEVGSFLSSGVDSSYVTSVLKPD
                     HSFSIGFDDKTYNEAIEARKLTELLDLDNTAAVIDGDMSFKAFPLIQYHLDEPDSNPS
                     CVPLYFLANLASQSVRVVQSGEGADELFAGYQTYGFHTNSKFIRVIAQGLKKLPKGTR
                     YNLGRKIGKMKNFHGRIHLYESLAPAKEYFIGHARVFEESEASEVLTPKYQTAPSVDE
                     IMTVHYEKTEGIKDEVNKMQYVDLHQWMPKDILLKADKLSMASSLEVRVPLLDIEVMK
                     LAQQIPSKFLLNQNNTKDIFRQAANKHLPEEWSNRVKLGFPVPIKAWLKEEHGYEQVK
                     ALFEADFAKEFFDQEKIMKLLDDHHEGRKEEQRKIWTIFSFLTWYKVYFVDETIPQAE
                     AIDYVTV"
BASE COUNT         6475 a         4561 c         3701 g         6768 t
ORIGIN      
        1 ttttttatac acaaatcatt cgcttcaaaa caaattattc aataccattg tatatttttt
       61 acataataac aaattatatt tgtaaagtat taaaacttgt ttctaatgtt ggtatgttaa
      121 aactttacaa attctttaaa aaaagtgaca ataaatatag tgaacaataa ggagaggact
      181 ttttaatgcg tgttaataaa atcgattttc acttttcaaa actcagatat caacctgatt
      241 ttgaacattt ctcggatgaa gagttccaac agatcaagga acatactgtt cttcgttctt
      301 ataaaaaagg acagatcctt ttcgacgatg gtgatcccag acaacggttg tatatcttga
      361 ccaaaggact cgttcgttta gaacgttatg atgaaagtgc aacttattac tactatgact
      421 atgtgaatac aaataacctt tttcctatgt caggaatgtt tggagaagag acctacactt
      481 atacagctca agcgatgact gatattgaaa cattctacct tcctgtttcc tactatgaaa
      541 aattagcacg aaaaaatccc gaacagttga tctatatgat acaaaaaatt tctaaagtag
      601 ttgaaataca tgaattgcgt atccaaatcg gattgacctc tagcgccttt gaccgggtaa
      661 aacaaaattt attcattttg aaagaagaat tgggcgtacc aacaaaagat ggtcatatca
      721 ccattcctta tccgatcacc ttaaaagaat tagcagtgaa tagcggcacg acacgtgaaa
      781 ctgctggaca agtaatcaaa caactacgtg attccgggct gttagattat gacaaaaagc
      841 gttttacttt ctgtaagaca gctactactc cagctatgag agaataacgc gaacaaaaat
      901 aagcagtgac cctgataaat atcagggtca ctgcttattt tcctccctaa aacttctgaa
      961 agatccggca gttttagatc actggacaag actcaaagat tttctatgtg tgtttcagtt
     1021 atgtaatatt cactgtttgc aaaaaatact gttagattaa ctagatgaag cgtcttgtcc
     1081 tcatccgatc tagattattc gatattcctc gacaataaac gattactcag tgagggcaat
     1141 cagaggaacg gataatgata aataagctgt aaaaaatagc aaaataacaa taatgattaa
     1201 aagtatgtaa aaaatagggc tttgacaaaa acgtgtcaca gccctatttc gattaaacgg
     1261 tgtgtacctg tcaactcttc ggtcacaagt cataatgttc ataaacatga ggagctgttt
     1321 tttgaaattg ttccttgtga atcagagtta aacgacaggc ttacgaactc ttgattaaac
     1381 ggcgttcaaa agcttgcata cgcggtaata agaacagcca ggtcaaaaac atgagaagcc
     1441 gtttttcctg ggctgttctt attacttgat gcagggcagc tttttcacaa cctctttatt
     1501 cagataaaac caagcttgga tcagcattca actgatagct agcaaaattt cttttttgca
     1561 tttccacatg tgctgctgct ccaatcattg cagcattatc tccacaaagt cgaagagggg
     1621 gaatcactag ttctacctca gggagtttag ctgccaaagc atcctgcaat ccttcacgca
     1681 acccttgatt ggccgctaca cctccagcta taatcagttg cttgactgga taattttgac
     1741 aagctcgaag tgttttagta atcaatacat cgatcacact tgcttgaaag ctagctgcta
     1801 aatcattttt gtctagatct tctccacgct gttgtgcatt atgcaccaaa ttgataaatg
     1861 cactttttaa tccgctaaaa ctgaaatcat aattatcttc atggatcatc gctcgcggaa
     1921 aatggtaatt atcttttccc tgatgagcta gctgatcgat ttcttttccg cttgggtaag
     1981 aaagacctaa tactcttcca actttgtcat aagcttctcc tgctgcgtca tctcttgtct
     2041 ccccaatgat ttcatatgat ccatcttctt gcatatagac taattctgta tgtcctccgc
     2101 taacaagaag tgccatcaat ggaaactgga atggtttaac cagacgggct gcatagatat
     2161 gccctgccat atggtttaca ggaataagcg gcaattggtg tgcccaagca aaagccttag
     2221 ctgcagaaat accaatcagc aacgagccga caagtcctgg tccataagtg acagcgactg
     2281 ccgataaatc ttctgccgac acaccagctt ctaccagtgc atcttccaag cataaggtga
     2341 tttgttctac gtgatggcgg ctagctactt caggtactac gccaccaaaa cgtttatgac
     2401 tgtttatttg agaagcaacg atgttagata agatctctgt tccatttcta acgaccgcta
     2461 cactcgtttc atcacaactt gattcaatcg ccagaatcaa ttcttcattc atttcatcgt
     2521 ctccgttttt aactttctgc tcataatgat cgcatcttcc gtcggattgc gataatatga
     2581 tttccgttta cctaatatac gaaacttttc tgattcataa agttttcttg ctgcttcatt
     2641 agatagacgt acttctaaaa aaacatgcag cgaatctttt atttgttccg taatcagtaa
     2701 ttgtaacaaa tgccgtgcat gcccttttcc ttgttctgaa actgcaacag caatatttgt
     2761 gatctcgact tcatctatta ctttcgagta tccaataaag ccacagatct ggttatcttc
     2821 agctaaaagc agataatcag agtgaggctg ttggatgtct gccaaaaact gcattttcgt
     2881 ccaaggtgaa ccatgtggat aagcattttc gctaatcatc catacgcatt ctgccaagta
     2941 cgcttcatca aaatcttttt ttctgcaaat caactaatct cactcatttt ctaaaagttt
     3001 tatcatatcc aacgcatctt cttttgtttc agtgtaatat ctttttttga tcgttgttgc
     3061 ttgaaacccc aatttccgat aaagtctttg tgcatcttga ttactcatac gcacttctaa
     3121 tgacatcgtc tcacagcgat tcattatagc aaagttctcg atttcgtcaa taagcagcga
     3181 accgattcgt ttacgctgat atgccggtcg aacggcaata ttcgtgatat ggcaatctaa
     3241 accgaacgct cttgaaccga taaaaccaat cattcttcca ttatcaaaga tacctagata
     3301 tagatgagga agcggtgact tcaattctga tataaaggca ctttttgtcc atggcaattc
     3361 gccattatag acttctcgtt cgacatccag cagctctttg atatcttctg tagtcaattc
     3421 tcttagtacg taacgacgat cgatttgttc gactgttttt tctgtataaa tcattttatc
     3481 ttgaaaaaag cttctggcat acgctctaaa ttttttcaac ataactttcg tctccaggtg
     3541 tgtgcgtctc caaccatttt tcttctgctt ctactcgttt taaatagcga ggaagaaaag
     3601 cttgtttctc tgcaactggt tcaactaaag cacctaattg agccacagtt acaccacttg
     3661 gaatatccca tgctggaact gtattaatca tcgcactagg caatatttcc tttatttcag
     3721 agaagaattt gtgacaatca ctcccgacga aataaatagc ttcattcagc ttttgtagtt
     3781 gtgtgaacaa atcagaagca gaaatatgta catcttcaag tacagtttct aatctatttt
     3841 ctttccagcg gtatgcccca gcgtatacat tttttcttcg agcatcaaac atgggcacaa
     3901 taatattatc cacaccgatg caattagccg cgatggtttt cagactagat actcctacta
     3961 gctctttatc taatgtataa gcaatcgttt ttgctgtcgt tacacctata cgaagccctg
     4021 tataagaacc gggaccatca gaaaccgcga ttcggtcaat gtcttttgga cttatttgaa
     4081 gatctgcaaa caattggtcg attgcaggca tcagtgttac actgtggttg cgtttgatat
     4141 ttgtttgtat ttgtccaaga atttgttcgt cttctaccac accgatcgcc aaagtttggt
     4201 ttgcagtatc tattcctaat gtaatcatat atttgatcct ttctgctatg acattctcgc
     4261 cttccattgt agcatacttt tttattctga ggatgaaaat accaaaggca aaactagtac
     4321 aataatacat gattatctgc tggcgggcaa attttgcccc ttcctgaaac acaaaaaagc
     4381 tgcggtatca ctccattttc ccacagcttt tccatctttt atttcgttga ttctcgaata
     4441 atcaactctg tttccacaat aatctttctt gaaaccggtg ctttggaatt acttaattga
     4501 agaatcgtct ccactgctag ctctcccatc cattcggtat aaacttttac agtagaaagc
     4561 gagggcgtta catatttcgc tacactgaca tcattgaagc ccatcacaga aatatcttct
     4621 ggtaccacta aacctttttc ttgaatcgct ttcagtgcgc caatcgctaa tgcatcatta
     4681 gctgcaaaaa gcgcatcagg aacatgcgga ttcgttttta aatattgctt cactgcttga
     4741 tatcctgact cgacagaaaa atctgcttcg ataattggac cttcgtcgta gcctttttct
     4801 gccaaaactt gttgaaatgc cataattctg ggatctttta gccgctgatg gtttttcttc
     4861 gtatattctg ttccagcaag catagcgata tgttgcgctt tatgggagac aaagtgttca
     4921 atgacttttt gaacacttga cttaaagtcg acaactagag agtcgattcc ttgagaagtg
     4981 ccgtcagaat ctaccaaaag caaggcattt tgggatcctt tcatttctaa taactgagct
     5041 tcgtcgaatt ttcctagtgc gatcgttcca tctgccggaa gatcactaat ggaatcccac
     5101 gtttctttca ataaagtaac gtttgcttct tctgcctttt tttcgattcc taggcgaata
     5161 gataaataat aaagatcttc aagctcttcc gtatcgtcgt accactggat caggcgaagc
     5221 gtttgcttag tatttgctgc atttttacga tgtttcgtat aatttaagcg ttctgctgct
     5281 tcaaaaattt tttgctttgt ggctaagcca acggaaagat caggatcata gttgagaact
     5341 cttgaaacag tagcaggaga aactcctgcc aactgcgcaa tgtctttaat tgttgccatt
     5401 tgtctctcct ccttttttac gtttataaaa tcgcagtaaa tcgttcaaac gctgcctgtc
     5461 ctttttcatc tcgcttgaat actccagcgt cttccagtac acgtgcaaaa acctgaccta
     5521 cagatttttg tacgacatcg gtgaccgtat ctttcgtcaa atcaggatgt tcttttttta
     5581 attgttctgc ccacggacga tgatattcag ccatctggtt ttcttctcct aataagaaat
     5641 gctctacttc tactagttct ggtttcaaac gaggtggtaa aacagcaagc cccattactt
     5701 cgatcaaccc aatgttctcc tttttgatat gttggacatc tttatgtgga tgaaaaatcc
     5761 catcaggatg ttcttctgaa acattgttgt ctcgaaggac aaggtccaac tcaaagtaat
     5821 tgtcccttct tcgtgcaatt ggcgtaatcg tatggtgtgg tgtaccatct gctgaaaaag
     5881 ctctgataga tactgtttca tccgaatatg ctttccattt ttccaatact gagttagctg
     5941 cttcaataag caagtttttc tctgttgatt gcaaacggat cacagacatc ggccatttca
     6001 cgactcctgc gatgacttgc ggataatctt ctagtttgat aagtttttct atcggtgctt
     6061 ttgccatagg aaattcatga cgtcctgcct gataatgatc atgagaaaga atcgatcctc
     6121 caacaattgg taagtcagca ttcgaaccaa caaagtaatg aggcaagact tcagtaatcc
     6181 gaagcagtct agcaaatgtt tcctttgtga ttttcatcgg gcggtgttgt tctgataaaa
     6241 tgatcgaatg ttcattgtaa taagcataag gggaatattg gaatccccag ctttcaccgt
     6301 ctagattcat tcgaataatc cggtgattgg tgcgggctgg atagttcaag cgacctctat
     6361 atccttcgtt ttccatgcaa agcatgcagg taggatagtc tacttttacc gcctcgcgtt
     6421 ctgctgcgat ttgttttgga tctttttctg gcttagataa attgattgtg atttccagtt
     6481 caccatactc cgactctgcc ggaaatttga tgttttttgc gatggctctt gttttgatgt
     6541 agtcattttt cttacttaaa ttaaagaaat actcagttgc ttcctctggg cttttcgaat
     6601 agtgctgtgc aaagaatgca ttgacgactg aaggtggcgg tgtgagaaaa tccattaatt
     6661 gcgcttcaaa aatctcttgt tctgctggca attctgaaag aacaccattt tctcttgcct
     6721 tagcgacaag ctgatccaat aagtctacag attcttcctt aactggtctt acttcaatgc
     6781 tttctagagc atcttctcct accatcccta aaagacgatt ctgtaaatat aagcggtcca
     6841 tttccatcca tccgccagct tcgatcgcaa gtgtggcaaa atcacaaatc gtttgactga
     6901 ttgacatatc tcattcccct attcttcata accatttgga tggctgacat gccaatccca
     6961 tgctgttttg ataatcgctt tgatatctgt gtattcaggt ttccagccca gaatctctct
     7021 cgctttttca cttgaagcga ctaatcggct aggatctcca gctcttcttg gtgctatttc
     7081 tgctggaatt tcttttcctg tcacttctct agctgcttct agcatttcct tgactgagta
     7141 tcctttgctg ctgcctaaat tgaagaagtt gctttcattt ccttctttca agtattccaa
     7201 agctaaaata tgtgcagcaa tcaaatcttc tacttgaaca taatctctga tacatgttcc
     7261 atctggtgta tcataatcgt ctccataaac agccaaggct tttctttgac caagagctac
     7321 ttgtaaaatg attgggacaa gatgcgtttc tggtgtatga tcttctccga tagatgcatc
     7381 tgcttttgct ccggctacat tgaagtagcg caaagcgaca tagcgcatgc catatgcttg
     7441 gtcacaccac ttcatcattt tttccatcat caatttactt tcaccatacg gatttttagg
     7501 attagctggt gtatcttctg tgattggaga ctctttcggc tctccataag ttgctgcagt
     7561 agaagagaat acgattttat tcacgttgaa ctcgtgcatg acttcaagta agatttgcat
     7621 accgtatacg ttgttgttga aatacatcaa tggtttttcg acagattctc ctactaaaga
     7681 gcttgcagca aaatggatta ctccttcaat cggttccttt tcaaatacgg aacgtagaaa
     7741 ttctttatcc cggatgtctc cttcataaaa atgagcatct ggatgtactg cttgtttgtg
     7801 acctgtgagc aaattatcta ccacagctac ttgatacccc ttttgaacta gctggtctac
     7861 agcgtgcgaa ccgatataac cggctccgcc tagtactaaa attgtcataa aaaacactcc
     7921 tttgattagt aaaattgtgt ttgttgattc tatataattt aaaaaacgaa gtttactttt
     7981 atggtgaaaa caaacaaagt aaaatttcgt aaattcatat ggtaaattta taataaaaca
     8041 tttagtaaac attttcaagg gttttttgca aaggtttaca aacaaacgta aaactagcct
     8101 ttttttctct tttgttccat aatagatata acaaataata aatgaggtgg attcaatgaa
     8161 aaaatttgta tctggaatat tagtaggaag tctggcaaca gccgctgccg ttgctggttt
     8221 ggttgcttct gtgaaaaaaa cagtgatcga tccaattgac gaaaaagaag caatgatcga
     8281 agaaaaccgt aaaaaagcaa tgcgtaaacg cgtttctcgt taataattat ttaaaaatac
     8341 gcatgaagac tttctaaaaa acagataagt catcatgcgt atttttattt gtcagtcaat
     8401 ataagttgtg ctattttatc tgcctgtctc agattccatg gatctgtata aggtctagct
     8461 actgctgcag ttagccaatc cacaccttca acaggtattc catagcaata aaagtcagac
     8521 tggatttccc ctgaagcatc aatcacgcga ttcgtacctc gctcaacatc tatagcttct
     8581 gaatgataac ttcctgtatt atctgaaaat gaaaaagggc gaacaagttt gttttccttc
     8641 aaatttttta tgacaggatt acttgaacga gaaaagcttg tttgtggcaa tcgagcttca
     8701 atcaaatagc gactgctgat ttctttatct tgtagagaaa taacatagcg gttgtgcttt
     8761 tgctgaatat caaatggcgg ttcgatcaaa tgaaaaatac ccgcatctat cagtgctgct
     8821 aattctttcg tccgaatgat tggcggaccg atcgttaaaa agctatttag atgagtaaac
     8881 catccccata gtatttcttt gtattcctta gcagaaaaaa cgtgccattc aatagcctgc
     8941 cggattggat cacgccagtc ttttaatgca tcaaatgttg aagcaaatgc tcctgtgcaa
     9001 ttgccttttt ccgcttcttt tatctgccct tcaataaata ttcggctagc ttctgaaaag
     9061 ggctcattct gataggcaag aggagtttct aaatacgacc aatcccatct ataagggatt
     9121 agtgctggat gctttccgag acactcctct gaagagaggg agagaaaatc atgctcaaaa
     9181 tttttctttg gaatcggcaa gtgatattct tcgatgattt tcttgtaata aaacaactca
     9241 acgtcttttt tcagatagtc aaaaaatgtt tcgccagtca aaaatcccga ctgatgccat
     9301 tgctccaggt tttcttttgt tagtaaccgt ggccatgcca ttgctccgcc ttgcttttga
     9361 tttcttcctc gagggtaata agggagcccc cttcttgatc cactgtatac aatcggttcc
     9421 ttgcctgatg gatgatagac cagttttccc ttttcgttct cgaagcgccc tcctcttgat
     9481 actgtaaaca atccgacata atcaaaaaaa cttaagccta gacctcttaa aaagacattt
     9541 tctttaggcg gtatcttcga aagatctaca tctgcaggat tagaaggaag ctgatacagt
     9601 aaaacatttt tctttgcgta gtctacccac actctttctt cttctgtctc atcattttcc
     9661 cagtgaccgc ttgctaaaac aagctgatcg atagaatagc tttgctgatc tgtctgtaaa
     9721 agataaccat cttttctttt gcttactttt tttactaaag aataaacaaa ctgaatagaa
     9781 gctggaaatt cttcttgcaa ctttgagaaa aaccagcgtt gatagagtcc gtataaacat
     9841 cgagtagatt gctgatcttt ttccagacga ttgatttctt ctataattag ttgcttattt
     9901 ttggttaccg tctgttcgat ataatctgta gcaggtcctt tactccattg aaacaagttg
     9961 ggtccctgtg ttaccaatcc tccactggac aatgtttcat ctgtaaaaag cgtcacttgt
    10021 tgagaaacag aattcattaa taactccggt ggctgattca accgccaaac tcgacctcca
    10081 ggaccattgg gatcgaaaag aaaaagttca agagaatgtc tgtcctgatt attatggaga
    10141 atcctttctg ctacacttaa tcctctaggt ccggcaccaa tgatccctat tttcatttgc
    10201 atgcctccat ttcgataaat tttttcaata aaacagcatg gttctgatgt tcattttttg
    10261 caccataaac aaaagtaatg actggatgtt ctttcataag ttgactgagt ttaaaaatag
    10321 cttgcttctt ttcttcttcg aaaatcaatt cttgctggta tttctttgca aatgaagcaa
    10381 atcgttctgg tacatggtca aaagttttgc gtgcttcatt actaggtgca atttctttca
    10441 accacagatc taactgcgct ttttcttttg aaattcctcg tggccagaat ctatccacga
    10501 gtattctata gccatcctct ttttcagcat caaggtaaac acgttttaat ttgatcatag
    10561 ttgtttcctt ctttctacct tttcgcttag cttatatatg tagataatag cacaaaataa
    10621 aaaaaatgaa cggcacaact atccattgcg ccgttcatgt gatcttggtc aaaatctaat
    10681 gattgattcg gtttttaaag ctgtcataca atcatctgtt ttatactttt ttacttttga
    10741 tttttccaga ccatccgtca tatccaccct taagtaaata aatctcttga taacctgctt
    10801 tacgtaattt atttgctgta cggatgctca aacttttctt ttgatcgtaa atatagacag
    10861 gctggtcttt tcgaatagat cctaatgaat ctttcaaaac agtgtaaggg atgtttcttg
    10921 cacctaaaat atgcccagca tcaaacacat ctttttcgcg aacatcgatt acttgtgctt
    10981 ttctcattcc ttctcgaaat tcttcttctg tcaaagtcgt tgctgaacgt ttggccatta
    11041 ctcgtagata gacttcccaa aggatgattg caagcacaat caatagcaaa atgatattaa
    11101 ttacccataa aaccatgtaa tgcctccgtt tttttaagca aatttcaagg ctaatgaggc
    11161 agcaccaatg acaccagctt cattaccaag ctctgctaat ttgatttttg ttgattctct
    11221 tacttgcggg aaagtaaatt ctttaaaata tttctctaca cggctacgta aaaattctcc
    11281 tgctgccgat actcctccac ctaacacgat acttgacgga tttaatgtat tccccaagtt
    11341 tccgcaagct aaacctaagt agaagcatac gcgatcaaca accatcaatg caaacggatc
    11401 gtcttcttga gccatttcaa agatatcttt acttgttact tcttctccgt tgtctagcat
    11461 tgctttcagt tttgaatccc ctgcgtattc ttctgccaag aaacgaccta ggcgtaccac
    11521 acctgtagcg ctagatacag tttctaaaca tccgcgttta ccgcaagtac attcgaaccc
    11581 ttcaggatcc acagtgatat gtccgatttc tcctccagct ccgctcacgc cgtgaatcaa
    11641 atttccgcca gcaacgatac ctccgccaac acctgttcct agagtaacga atacaacgtc
    11701 tggatcgttt tctccagcac ctttccatcg ttcacctaaa gcagcaacat tcgcgtcatt
    11761 atcgatagcg aatttgattc ctgttcctgc ctcgatcagt tttttcacaa attgaacttc
    11821 tgtccagttt aagttatacg caccaataac cgttccagct tcgctgtcca cgcttcctgg
    11881 ggtacccatg ccgattccga taaagtcttc ggctttcata ttatataaat tcaaacgatg
    11941 attgattgat tcaatgattt caggaacgat atgcttacca tcatccaaaa tattggtatc
    12001 gatgctccat ttttgctgaa tctctccttc tggtgttaaa attgcaaatt ttgctgttgt
    12061 tccacctaaa tcaatcccaa taatcttctt gtccattaca aatttccttt ctttcggctt
    12121 tcttcaattc ggtgctctct ttttaatatg cgccacgctg tcatgtatgt atcatgatca
    12181 atcaaacggc tatcatacag attacgcaat tcgatagtca ttaattcaat gtcatagatg
    12241 cgtgcaccca catagataaa gatgccaaat tttttgaaca gctgctgtac atcatataag
    12301 ctctccatct ttaagcactt ccttcatatt atacaagtaa accatatttt tttaaaccca
    12361 gtaaaataca tattacaaaa agaaatacaa aaactagtgt agaaagaatc ctttggtgta
    12421 tcgaatagct tgtctgttgt ttcggtagac ccaaaacatt ccctaacact aatccgccaa
    12481 ttagcccacc gacatgtccc caaacatcta cagaggtatc cagaagacca aaaacaaatg
    12541 tcaacacaat aaataaaaga aaatgtctga ctagctgctg gatcgctggg ttatgtttaa
    12601 aatgaaaccc taagataaac aacgcaccga acaaaccgaa tatcgaagta cttgcaccac
    12661 cagacagaac attcgcttcg ttaaaagcaa agcttgctgt atttcccatt atcccgctga
    12721 acatataaat caagaaaaaa cgccaatggc catagattgc ctcgatttgc tgccccataa
    12781 aatacagtat cagtgagttc attacgaaat gcatcagtcc aaaatggatg aagattggtg
    12841 tcactaaacg ccaccattca ttgaagtgaa caagataagg accatacatt ccggcataat
    12901 aagggatctc aagtcgcgga aataattcca tcatgaggaa gaccgctgtc tgtatcccta
    12961 aaaaagcata ggtccataac ggtcgtctta accatgcacg caattttatt tgctgttgat
    13021 aattcatgaa aacctcccat ttcattttag tcaataaaca attgttggat cttctgatca
    13081 aagctctccg gttgccattg ttcattcaat tgttcacgaa aaacaaggct acatgttttt
    13141 ttatgaaaat tatttaaata ccggtcgtaa taaccaccgc caaaaccgat tcgataaccg
    13201 gctctattgt agataatacc tggtacaatg aggagatcga tcatctccgg caaatagagc
    13261 ttctctgatt gtggctcaag aacaccaaaa ctcgaacgta caagaacgga ctgttcatcc
    13321 atttcataaa acgccatctg acgtttcggc aaagttttag gtacaactat tttcttttgt
    13381 tctttttctg ctcgttggat aaccggctgt gtatccagtt caaattcatt cgataaagtc
    13441 gtccctatca cttttgctga cttccagtac tcgctggcga atagctgttt atagattcgc
    13501 tgttcttttt cctttttctt gccacttttg gccagatttt tcagcttttc gattcctttc
    13561 cggcgtaatc ttactttcaa ttggtccaaa aactcaccct tccttctctc ataaaatggt
    13621 ataggaaaaa aagaaaaata gcaatcgaac tttcaaataa atgcataaat agaataaaaa
    13681 gaagacctag aaatgattgg ccttcctttt ttaatgaatt tcaactgttc agcgcgtgtt
    13741 tttgtctgat aaaaagtgca tacagaaaat atggcgcacc aaaaagggca atcaaaattc
    13801 ctgtaggtac ttccccactt ggtagaatca ctctagcaac catatctgca ctaagcagaa
    13861 aaatggaacc gaacaaaccg ctcagaacaa aggtccagtt attttcttta cgtacgacta
    13921 gcttcgcagc atgaggagct agtagtccta caaaagataa actacctgtc accgctacac
    13981 taccgcttgc caaagcaaca gctaacaaca aaaggatttt ttgactttta gccacgttaa
    14041 ggcctaatcc ttgcgcgcgt tcttgaccaa atgatagcaa tacaatcctc tgtgcataaa
    14101 gtcctcctag cggaattaag ataaccgccc atggaagcaa tgcccaaaca taaggccagc
    14161 tcgcacccca gattgatccc gctaaccatg cattcacaaa tccataattt tccttagata
    14221 attggatcgt gcatagcaat gttaatgcag aaaggccggc attaacggca ataccggaca
    14281 ataagattcg attcataccc aaaaagccat ttcgatcata ggccgcaaga tagacacagc
    14341 aagcagctaa aacaccacct agaaaggcta caactagcaa tagaaacggc agcgaatagt
    14401 gcgaaaagaa acctaaataa agcatcacta aaaatcctgc tccggcatta atgcccaata
    14461 tactagaatc tgccaattca tttcttgtta ctccttgcaa gatataacca gaaacgccaa
    14521 gacaagctcc tgctaataaa ctgatcagta atcgaggaac tcgaaattct ctaacaatca
    14581 gcgccaaaga agatgattct ttccctatga acgtggcaaa aagctgatcc aggtaaataa
    14641 aaggcgtacc aatcattaaa ctacctatag acaggatgac aagtacaaaa agcaagaaat
    14701 agaacgtcca atatattttt ttcaccgttg ttccctcctt acttgtatca gtaaaaacgg
    14761 aacgccaatc aatgcaatga ctaatccaaa aggtgtttca aaagggggat tgatcatcct
    14821 cgctgtaaaa tccgccaaca taaccaatcc accgccaact aaaaaagaga caggtagaac
    14881 ataacgatag tccgttccaa ccattccgcg aacaactggc ggaaccaaca gcccaataaa
    14941 tgaaacaggt ccgactaacg aaacggcaat ggctgctaac actgcaacta gacagaaaac
    15001 aatcaaccgc gttcgtttag gatgacttcc aagagctaat gcatgtgttt cactcaaatg
    15061 aatcaaagat attgcttttc ctaatgaaaa agaaaccaaa acagcaagta tatataaagg
    15121 aaacaatact gctaattgtt tccatgtgat attggcagct ccgcctacaa accaaaaagc
    15181 aagatcctgc tgcagattaa agaattgggt aagtgcttgg ctgatagcag taaaaaaaga
    15241 actaatagct acgccagcta aaatcagctg taaaggattc atgcccattt tcgaaaactg
    15301 caaaaaacca aatacgagca aagttacagc aaaagaacct gccaatgaca ttagaaacac
    15361 agaaaaatca gaagctttag gccaaaaagc aaaactaagg gccattgcca acgaagcacc
    15421 cgcgttgatt cctagtaagc ctgaatctgc taaaggattt cttgtcaccc cttgcatcaa
    15481 tgcaccgctc aatgcaaaac aactccctac aagaaatgct cccgccacac gaggcaaacg
    15541 aatatttcgt atgatttggt gactttgttt tgatctatca aaatcaaaca acgcctgcca
    15601 aaaatctgct ttttccacag tcgctgcgcc atttgaaaga gcaacaaaaa aaataatcaa
    15661 aagaacaaaa cagaccaaaa gcaggagtct atttttcttc atagtcctca taattgatcc
    15721 tcctgctctt ttggcaacaa atcataggtt agaaaaactg gtttatttgt tcttggaagc
    15781 gtgacaatcg ttgcatcaat ctcaaatatt tttttcatac actcttttgt gatcacttct
    15841 tcaggtgtac cttctgtgat caacttaccg tttttcattg ctaataaata atctgaaaaa
    15901 cgtgaagcat gattaatgtc atgaatcgac atcaagatcg ttgtttgttt catttgattg
    15961 atttcagtca ataactgtag gatctctaat tggtgtgccg gatctaaaaa tgttgtcggt
    16021 tcatccaaga tcaagatatc tgtttcctga gcaagcgcca tagcgatcca gacacgctgg
    16081 gtttgtccac cagacagcgt tgcgatatcc cgcatacgaa atccagctag acctgtagca
    16141 tccaacgccc actcaattgc ttcatgatct tgcgtgtcca tgccactgaa cagtttttga
    16201 taagggtatc gtccgtaaga tactacttct tctactgtca tacctgtagg gtgttcacta
    16261 gtttgtgcta ataatgcaat tttttttgca agttcttttg aactgtatgt agagatatca
    16321 gagttattga caatgatctt tcctgatttc acaggtagta tacgggtaaa acttttcaat
    16381 aaagtagatt ttccacttcc atttggccca atcaaactag tgattttccc ttcaggaatc
    16441 gtaacagaca tgttttcaat gatttttttg ttttcgtagc cggctgcaat gtcagctgct
    16501 cttaatctcg tcatttcacc catcctttcg ctgaaaatca ttctcaatta tgacgctgtt
    16561 tcaacatttc gtcaagcgct actttcatat ctatagccaa atgaagcggt tgacagcgtt
    16621 cgaaatatca ggaataataa ccaatgagaa agattctcaa ttatgttagg aggaaagaaa
    16681 tgaaattaaa aacaaaagta ttcacattat ctagcatatt gctgctggct ggagtactga
    16741 gcgcttgctc cccctcttca gataaagaaa cagctacaac tgccaattcg acgacttcat
    16801 ccaatagtac tcatacagtc acagatacac ttggacataa agtagagatt ccaaacgcgc
    16861 ccaaacggat catcggcagt tacttagaag actatctgat tgcactaggg gaaaaaccta
    16921 tcgcccagtg gactgttggc agtggttcca ttcaagatta tctgcaagat gatttaaagg
    16981 atgtcccaac gatttcttat gaccttcctt atgagaaagt gttaagcttt gagcctgatt
    17041 tgctgttgat ttcttcttcc gctactgtcg aaggcgggaa atacgaacaa tatagcaaaa
    17101 tcgcgccaac ttatgttgta aaaaacggaa cagatgtcac atgggaagaa caactaaaag
    17161 atgtcggcaa agctttggca aaagaaaaag aggcagaaaa aatcatttcc gattatcaaa
    17221 aatccatcaa gaaaactcga gaagaactag cagataaaat cgagaacaag acagctgcag
    17281 tgttgtgggt gaccaacaac tcagccttta tggtcagcaa aaatcggtca agtggaagaa
    17341 tcgtatacga cgatctggaa tttggtgttc ctgatctagt ggaagaggtc tccaaaaaag
    17401 ccacttctga ctggtctgct gtttcctctg aaaagctagc tgaattagat gctgactaca
    17461 tcatccttgt gaacagtgac aaagatgctg ccatgttcaa tgaaccaagc tggaagaatc
    17521 taaaagcagt gacagacaat catgtactgc aattcggacc tgaatctagt tggctatata
    17581 acggacctat tgccagtcag gaaatggtca atgatatcaa agaaaaacta aattgatttt
    17641 aactaaaaaa agcatcttca cgattagtcg tgagatgctt tttttcttat taacaaacta
    17701 gacgcataga ttttattctt cttttgaagc aaccctattt tttactcttt cccatttttg
    17761 aaaacgatgg gcatatagat tttcttgatc cgttatcccc tcatactcct ctataagggc
    17821 gaagtctgaa aaatcaatat tcccaatata cgtatcgcct tcaaacgtgc tatcgatcaa
    17881 tgttcgccag atccgaccag tgtaaggaag aagttcttga aaaatccgac taccacccga
    17941 aacatagata tcttctgaga tcgtttgact atacgacaaa agttcatcga tcgaatgcat
    18001 tacttccgca ttttctttcg tgcgaaaatt tggttgcgtt gtcaacacga tgatatggcg
    18061 ataaggaagt gaaagatccc ccattccttc ataggtcttt cttcccatca ctaaaattcg
    18121 atccattgta tgttcgcgaa agaaacgcat atcatttggc aatcgccatg gtaacaaacc
    18181 atttttgccg atcagaccat ttttatcttg tgcccacatg gaaataaaca tattatccgc
    18241 tccttatgat tttagatgtt ttgagataaa aaaagtctca attctttgac cagcaaggaa
    18301 ttgagaactt taaaaacatt attttgtttc acggtgcaaa gtaactttgc gttcgcgtgg
    18361 gcaatatttt tgtttttcca aacgatcagg attgttacgt ttgtttttgc ttgttagata
    18421 gttacgttct ttacaagaag tacactctaa agtgatgttt acgcgcatgt ttttccctcc
    18481 catttttaca agaatcctct aagatgactc tcagacttga ttatcttacc atgatttttt
    18541 ctagattgct agtgtttttc acgattttgt taagtaaaac tacttgacga taaatacaac
    18601 ggttttactt ctcaaacaaa atctttttct ttttgtataa atacccttac acttgtttat
    18661 atagattatg ttttttaata aagggggata ctaatatgcc atggaacatg aaggactttc
    18721 ctgcatcaat gaaaaactta gataaattga caagaaaaaa agcaatcgac atcgcaaatg
    18781 cattattaga tgaagggtac cctgataacc gtgcgatccc aattgccatt gatcaagcaa
    18841 aaaaatggta tgaagatgct agtgaatcag aaaaacgaac tttcgaaaaa gaaaaaaatc
    18901 caagcaagac ggatgaacac gatacgaatc ctcgtgctgg aaaactgctt gattcagatg
    18961 tgatcgtaga atatgaggaa gaacaatgga tcgtgaaatc taaaggggca aaaaaggcaa
    19021 gcaaccattt tgatacaaaa aaagaagcaa tcgaaaaagg aaagcaagta gcacaaaaca
    19081 aagaatcaac tttgttcatt tataaaaaag atgggacaaa agaaaaagaa atcgattatt
    19141 gaacatatgt aaaggggttg tgacgaaaat cgtgtcacaa cccccttatg ttcgaaacaa
    19201 tggtgttcct ctgccaactc cgaaccataa gtcataatat ctataaaacg ggaagagctg
    19261 ttttttgaaa ttgtttctta tcactcagtg ttaaacggta ggagaaaacc tcttttttat
    19321 tgatatttga ttttcatcaa acagtcacat agtcgattgc ttctgcttgc ggaatcgttt
    19381 catctacaaa gtagacttta taccatgtca agaaactgaa gattgtccag atttttcttt
    19441 gctcttcttt acgtccttcg tgatggtcat ctagcaactt catgattttt tcttgatcaa
    19501 aaaattcttt tgcaaaatct gcttcgaata atgctttgac ttgttcgtag ccgtgttctt
    19561 ctttcaacca agctttgatt ggcacaggga atcctaattt cacacgattc gaccattctt
    19621 ccggaagatg tttgttcgca gcttgtctga aaatatcttt tgtgttgttt tgattcaata
    19681 aaaacttact tggtatttgt tgagccaatt tcatgacttc gatatccaat aaaggtactc
    19741 ggacttccaa agaacttgcc atagaaagtt tatctgcctt taacaagata tcttttggca
    19801 tccattgatg aagatcgaca tattgcatct tattgacttc atctttgatt ccttctgttt
    19861 tctcgtaatg aacagtcatg atttcatcca cagatggtgc tgtttgatat tttggtgtta
    19921 acacttctga tgcttctgat tcttcgaaca ctcgagcatg accgataaaa tattcttttg
    19981 caggagcaag tgattcatat aaatggatcc gtccatggaa gtttttcatt tttccgattt
    20041 tacgtcctaa attatagcgt gttccttttg gtaatttttt caatccttga gcaatcacac
    20101 ggatgaattt tgaattcgta tggaatccat acgtttggta tcccgcaaac agttcatccg
    20161 ccccttctcc agattggact acccgcacac tttgtgaagc aagatttgct aaaaagtaca
    20221 aaggtacgca agaaggattt gaatcaggtt catctaagtg atactggatc aaagggaatg
    20281 ctttgaacga catgtctccg tcgatcactg ctgctgtatt atctaagtcc aatagttcgg
    20341 ttagttttct ggcttcaatc gcttcattat aggttttatc atcaaacccg attgagaaag
    20401 aatgatctgg tttcaataca gaggttacat aactggaatc aaccccgctg gataagaaag
    20461 aaccaacttc tacatcactg accgtatgtg cttcaataga agcttgaacg gtttcgtcga
    20521 ttttttcgat ccattcttgt cgggaatgcg tttcttttgt ttcaaaatct gcatcccaat
    20581 attgctgaat gtctattttt ccatctttat acgtataata atggccttca ggcaaacgat
    20641 agacatcttt gaagaatgtt tcgccgttta atggagaata ttggaaagtc atataaggtt
    20701 tcaacgcttc tttgttcaat tctttgttaa aatcaggatg aggcagaaaa cttttgattt
    20761 ctgaaccaaa cataaatgtt ccgttcattt ctgcataata atatggtttg atcccaaagt
    20821 ggtctcttgc accgaataac tcattctttt cattatccca aatagcaaaa gcaaacattc
    20881 cgcggatctt ttgcaataat tctgttcccc attcttcgta accatgcaaa aggacttctg
    20941 tatctgcatg tgtttgaaaa acgtggccag ctgcaatcaa ttcttctctc aaaggctggt
    21001 aattatagat ttcgccatta aaaataatga tctttgtccg atcttcattg taaataggct
    21061 gagtaccgcc ctctaagtca ataatactta atcttctgaa tcctaaagca acatgtttat
    21121 cgatatattc tcctgaacta ttaggtccac ggtgaacgat tcgatccatc attgtattaa
    21181 taattgtttt tttgttgtcc ttgtcgttaa caaaacctac aataccgcac atttaacttt
    21241 cacatcctaa tataaaatta ctgtttattt cattcatatg catcttatca tagaataaat
    21301 agcggacgaa tattttctct cttacaattt cgtcacgtga aaacaaaaga tgaaagtttc
    21361 tgtttttaca gaaaataaag tcccaatatc taatttacca tgttttcttt tttgaggcta
    21421 ccttattctc ttcgtgttaa aaaaatatta gtggatgcta tttccaaaaa aaccagcaaa
    21481 aaaagacacg acctaaagat cgtgt
//
LOCUS       DARBET010000044        20905 bp    DNA     linear   BCT 07-NOV-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4027
            SAMEA114569841-rid20112183.denovo.044, whole genome shotgun
            sequence.
ACCESSION   DARBET010000044 DARBET010000000
VERSION     DARBET010000044.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA114569841
            Sequence Read Archive: ERR12203666
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 20905)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 20905)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001972618.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001972618.1
            Genome Coverage       :: 132x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/07/2023 13:18:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,041
            CDSs (total)                      :: 2,975
            Genes (coding)                    :: 2,908
            CDSs (with protein)               :: 2,908
            Genes (RNA)                       :: 66
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 67
            CDSs (without protein)            :: 67
            Pseudo Genes (ambiguous residues) :: 0 of 67
            Pseudo Genes (frameshifted)       :: 26 of 67
            Pseudo Genes (incomplete)         :: 28 of 67
            Pseudo Genes (internal stop)      :: 20 of 67
            Pseudo Genes (multiple problems)  :: 6 of 67
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20905
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA114569841-rid20112183.denovo.044"
                     /isolate="VRE4027"
                     /db_xref="taxon:1352"
                     /country="Denmark"
     gene            131..505
                     /locus_tag="SCJ86_001790"
     CDS             131..505
                     /locus_tag="SCJ86_001790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287966.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-sulfur cluster biosynthesis family protein"
                     /protein_id="HEG1169331.1"
                     /translation="MYLKITEQAQERLQKYIDEGATVILDLDDGVGEYSKMGVCSLDT
                     SFRLLLLDKEQRKNDYKLQLDSDIGDVYIKDYSKMYMDEHMTLSLDPRLGVFTLESPS
                     GSLDSHVQLVDLRPEKFSGIKQ"
     gene            complement(619..1248)
                     /locus_tag="SCJ86_001791"
     CDS             complement(619..1248)
                     /locus_tag="SCJ86_001791"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287965.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HEG1169332.1"
                     /translation="MKIVFLHGLGQDEQSWKDVISYLPNSYSCKSLSLFNHLNSTDTA
                     TLDDLTKYIEVQLNQIKEPFILCGLSLGAVIALNYLATPSPYLKKVVLVAPQFESPNR
                     LLLTFQNIYFYCVPSKIFEKLGLSKKQTLTLLRSLETLDCKKQLRTSRLPKLILCGTK
                     DHANKPAAKRLYKLSTNGRLLFIKNGKHELNREKPEIMAQAMRSFIENS"
     gene            complement(1382..1454)
                     /locus_tag="SCJ86_001792"
     tRNA            complement(1382..1454)
                     /locus_tag="SCJ86_001792"
                     /product="tRNA-Thr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1419..1421),aa:Thr,seq:ggt)
     gene            1668..1741
                     /locus_tag="SCJ86_001793"
     tRNA            1668..1741
                     /locus_tag="SCJ86_001793"
                     /product="tRNA-Asn"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1702..1704,aa:Asn,seq:gtt)
     gene            complement(1879..2043)
                     /locus_tag="SCJ86_001794"
     CDS             complement(1879..2043)
                     /locus_tag="SCJ86_001794"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320198.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEG1169333.1"
                     /translation="MKNLIAGGFLFVGGGILMNTDNFGNVIPFLGAACALIGVGMLIY
                     FVFSDDGKYG"
     gene            2338..3666
                     /locus_tag="SCJ86_001795"
     CDS             2338..3666
                     /locus_tag="SCJ86_001795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287964.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="C1 family peptidase"
                     /protein_id="HEG1169334.1"
                     /translation="MKAITPDMTTKFNKQFQANIKQQALQRAVVKNGITASAENVSAH
                     VENTPVFSIDLATGKVANQMQSGRCWMFAALNTFRHKMLNQFHLKEFELSQNYTFFWD
                     KYEKANYFYENIIATGKEAIDSRKVAFLLATPQQDGGQWDMIVSLFQKYGVVPKSVMP
                     ESANSSNSRDLNNYLNKLLRKDAVVLRKMVAQGETLDAIEEKKEEMLESIYNFLAISL
                     GTPPKEFDFEYRDEEKNYHLDRGLTPQIFYDKYIGVKLDDYVSVINAPTKDKPFNRSY
                     TVEMLGNVVGGKEVKYLNVDMETFKKLAIAQLEEGESVWFGCDVGQSSTRTSGIMALD
                     AYSMDDLFDTDFTMTKAERLDFGESLMTHAMVLTGVDLVDGQSTKWKVENSWGEKVGK
                     NGFFVMSDAWMDEYTYQIVVRKDLLTKEQLNAFNEEPIVLSPWDPMGALA"
     gene            3868..4701
                     /locus_tag="SCJ86_001796"
     CDS             3868..4701
                     /locus_tag="SCJ86_001796"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008266492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fructosamine kinase family protein"
                     /protein_id="HEG1169335.1"
                     /translation="MDIQTVLSDLKLNGKVIPVVGGDVNQTYRIKTEHRAYFLKIHPN
                     VKKGFFEAEVDGLKELSAFVRVPDTYMLGETSEGAYLLMEWIEPGKGDQRDLAAALAN
                     LHQQTAPQFGFRKDNYLGTLVQKNSFEEDWWTFFFKDRLESQISLAEETNRWNVQRQE
                     KYLRFKERVLKSVEPKKITPRLLHGDLWSGNVFFDQQGHPVFVDPAVSYGNREQDIAM
                     SQLFGGFRPEFLDAYQTIFPLEKGWKDRLPIYQLYYLLAHLNMFGESYGSQVDQLLEN
                     F"
     gene            4717..5454
                     /locus_tag="SCJ86_001797"
     CDS             4717..5454
                     /locus_tag="SCJ86_001797"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287962.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-[acyl-carrier-protein] thioesterase"
                     /protein_id="HEG1169336.1"
                     /translation="MAKKYTKKHEVSYYECDINQTMTFPSMLGVVIKTSEDQSDQLGR
                     GSEFVNSFGLTWVITNYSMEINRLPRVGEVISVTTQAMEYNKFFCYRNFWIHDEDGKE
                     LVKIESVFVLMDFVNRKMSSVNEEIIAPFESEKIKKIKRQEKIEKIDSGFMLPYRVRF
                     YDIDSNQHVNNAMYFNWIIDVLGYDFLTTHVPEKVIIRFDKEVEYGNEIESHFEQIPI
                     ENGVKTKHEIRMQEQLYCEANIIWKNK"
     gene            complement(5609..6577)
                     /locus_tag="SCJ86_001798"
     CDS             complement(5609..6577)
                     /locus_tag="SCJ86_001798"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347874.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="asparaginase"
                     /protein_id="HEG1169337.1"
                     /translation="MKKILVLHTGGTIAMKENMLTGGVSPDVANPLLDAEITIPKHVE
                     LLVEDIFNLPSPHIMPSHMLVLKERILSAYASGISGVVITHGTDTLEETAFFLDTTIG
                     NKLPIVLTGAMRSSNELGSDGLYNFESAIRVASCEEALDKGVLVVMNDEIHSARYVTK
                     THTTNVATFRTPTLGPIGLVTKNRILFLQELLETKRLDISAVDGTIPIVKAYAGMQGD
                     LLEAIAHTKVDGLVIEALGAGNLPPQALAALEKLLVQKIPVVLVSRCFNGIAEPVYDY
                     EGGGKELEKMGIIFCNSINSQKARLKLLIAVNYGLSGEELISFIQN"
     gene            6809..7642
                     /locus_tag="SCJ86_001799"
     CDS             6809..7642
                     /locus_tag="SCJ86_001799"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002372832.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF72 domain-containing protein"
                     /protein_id="HEG1169338.1"
                     /translation="MIRIGLTSFKEHDKLTGKKNNTLYEYAGFLPLVELDTAYYGIPR
                     KSSVENWISEVPESFRFVVKVYQGISGQGDWKPYYQTEEEMIAHFLDVMSPMVDAGKM
                     FCFLVQFPSSFKCTKENVQYLRKLRKWFEGYPVSIELRDYSWYSDEFLERTRNFMSRA
                     AFSLAIIDEPQLLNTTIPFDPFVTNPNFALFRFHGRNAQGWQANDKEWRKKRTLYRYS
                     EKELQELAHEVKVVDSKTKEVGVIFNNNSGGDAAENALALQKILNIKPDGLNPTQMDL
                     F"
     gene            7676..8515
                     /locus_tag="SCJ86_001800"
     CDS             7676..8515
                     /locus_tag="SCJ86_001800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298857.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cof-type HAD-IIB family hydrolase"
                     /protein_id="HEG1169339.1"
                     /translation="MIKAIFFDIDGTLVTNRSKALESTKQAIEYARKNGILCGVATGR
                     SPVKIKEIIDELELDMYVVYNGQLVFTADRTIIDHPFEQKVLEHIVEFADENHRQIVF
                     GARNRLDGSTTMLLGQSIFIKRLVSFLPRKFPVRLMKKILQVFSPHRQKDRYETLEIL
                     KEPIYQCILLSPESEQKKLEHRFPECTFQRSNTYTVDIIPKGGSKLLGIQAFANAVEI
                     EMEEIMAFGDHYNDIEMLKGVGIGVAMGNAQIEVKHAADFVTQSNEEDGIYEGLKHFG
                     LIY"
     gene            8541..9101
                     /locus_tag="SCJ86_001801"
     CDS             8541..9101
                     /locus_tag="SCJ86_001801"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377553.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HEG1169340.1"
                     /translation="MEAETPFQKVETFHKTFDPRRPEIPTAFTENEAHTRAAFKIEEL
                     VEFVAATSPNKEQLHEAVMLLHEALDKAEEKILGKTQWGSDPLVEQVDALTDILYFTY
                     GSFSLLGIVPDRIFEIVHQANMGKLFPDGKPHYDPVTNKVLKPENWQMEYAPEPKIAA
                     EIQRQIQEAIAKKKSADIGMCTNEKE"
     gene            9300..11021
                     /locus_tag="SCJ86_001802"
     CDS             9300..11021
                     /locus_tag="SCJ86_001802"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736084.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="septation ring formation regulator EzrA"
                     /protein_id="HEG1169341.1"
                     /translation="MGNNVIIGIVIAIIIVAIVVFLVSYLMKKKNQDRLDALEKRKEE
                     LFDLPVIEEVDDVKKMHLVGQSQNTFREWSQKWNDISTSSFAELESQIFEVENLNESF
                     RFLKGKKAVVEAEATMDQMEKQVTEIREGLKELRETEERNSLAVQQALDVYEELKEKV
                     REDEHEYGPALFELQKQIKVIEKEFTQFVTLNTSGDPVEAREVLDKAEKHTYEVEALM
                     KKIPPLYEELHTTFPEQLEEISDTYEKLMEEQYVFPEEDLAEDIAKVSRRIENSLANL
                     EKTEVETVEFENRETADLIDSLYDILEREMEAQRYVKTNQSTIAEYIKHTTKNNRQLL
                     IELDHTSQSYTLNHNEIGRVRGFQTEVEEMERQNEQMIPQIRQHEIPYSEVRTFYKTV
                     FKVLEDIEKQQVEIDDSLHELRKGEKEAQEKIDTFEFKLRSLKRFVEKQRLPGLPNDY
                     LEFFFVATDRIEELSVVLNKIRVNMEEVNRLVALCEEDLELLDKKTHDLVDAAALTEQ
                     MLQYANRYRHTHEEVREAIDKSYYLFNKEYHYQEALDEIGTALERVEPGAFKRIEDFY
                     FNHPDLV"
     gene            11186..12328
                     /locus_tag="SCJ86_001803"
     CDS             11186..12328
                     /locus_tag="SCJ86_001803"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294077.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine desulfurase"
                     /protein_id="HEG1169342.1"
                     /translation="MIYFDNSATTPIFPQSLDAYVKTSQRIIGNPSSLHDLGNQANRL
                     LQQARKQIADLIHVEPEEIYFTSGGTEGDNWVLKGTMIEKREYGNHMIISGVEHPAVS
                     ETAEQLKELGIEVSIAPVDKRGFVRVDELKELIRKETVLVSVMAVNNEVGSIQPIQEI
                     SDLLDDFPKIHFHVDAVQAIGKVPFSQWLTERVDFATFSAHKFHGPRGTGFIYWKKGR
                     RLAPLLTGGGQERNQRSGTENVPGIVAMVKALRLSLEIKEQKPEQTQRLKVALINALK
                     TYEKVTIFSEGTDYAPHILCFALKGIRGEVLVHALEEKQIFVSTTSACSSRKHMASST
                     LHAMHVPNDLATSAVRISLDENNSLAEVEQFMIIFKQLYKKFSAIN"
     gene            12344..13555
                     /gene="thiI"
                     /locus_tag="SCJ86_001804"
     CDS             12344..13555
                     /gene="thiI"
                     /locus_tag="SCJ86_001804"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002391639.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA 4-thiouridine(8) synthase ThiI"
                     /protein_id="HEG1169343.1"
                     /translation="MKYSEIMVRYGELSTKGKNRKSFIMQLAQNVRQALKDFPEIKIH
                     ADRDRMHLLLNGADGQLVIPKLAKIFGIQNFSPSIRVEKDVEVLKQAVQDIMKEIYTG
                     KETFKIIAKRSDHQFELTSNELNQTLGNAVFDIFPHIQVQMRQPDIPLRVEIRRDGAY
                     LSYETIKGAGGLPVGTSGKGMLMLSGGIDSPVAGYFAMKRGVEIEAVHFASPPYTSEQ
                     ALQKAKDLTAKLAPYVGTIHFIEVPFTEIQEEIKKSVPQGYWMTITRRMMLRLTDEIR
                     RIRHGLVILNGESLGQVASQTLQSMVAINEVTNTPIIRPVATMDKLEIIEVAEQIDTF
                     NLSIQPFEDCCTIFAPPQPKTRPQLEKVHQYEERLAIDEMIQRALAGLKMEEIQAESS
                     NEKTEEFSELL"
     gene            14128..14775
                     /locus_tag="SCJ86_001805"
     CDS             14128..14775
                     /locus_tag="SCJ86_001805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341217.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="redox-sensing transcriptional repressor Rex"
                     /protein_id="HEG1169344.1"
                     /translation="MREKTDTLPKATAKRLPLYLRYLKMLDDSGISRIKSNEFSEITQ
                     IPSATIRRDFSQLGELGRSGYGYDVPFLIDVFNNILNTKEEKRIALVGYGNLGKALKH
                     NNFRRNENLNIVCVFDNDPALINRVIDGEMIYPIDRFAEIAKAKNVTVAISTVPSKYS
                     QSAIDEIVKGNVTAILNFAPDRVTVPAYVNVQYIDLTTELQTLIYFDENYSEVFS"
     misc_binding    14904..15120
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00230"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="T-box leader; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="tRNA"
                     /db_xref="RFAM:RF00230"
     gene            15168..17813
                     /locus_tag="SCJ86_001806"
     CDS             15168..17813
                     /locus_tag="SCJ86_001806"
                     /EC_number="6.1.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310214.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="valine--tRNA ligase"
                     /protein_id="HEG1169345.1"
                     /translation="MTEENNLSTKYNPQEVEAGRYQHWLEQDLFKPSGDKKAKPYSIV
                     IPPPNVTGKLHLGHAWDTTLQDMLIRQKRMQGYDTLWLPGMDHAGIATQAKVEAKLRE
                     QGISRYDLGREKFIDQVWEWKEEYASHIREQWAKMGLSLDYSRERFTLDDGLSEAVRK
                     VFVTLYEKGLIYRGEYIINWDPQARTALSDIEVIHKDIEGAFYHMSYELADGSGVVEI
                     ATTRPETMLGDTAIAVHPEDERYQALIGKKVILPLVNKQIPIIADEYVDMEFGTGVVK
                     ITPAHDPNDFEVGNRHDLPRVNVMDDDGTMNELAGKYAGMERFAARKAIVQDLKEIGR
                     LIKIDTMIHSVGHSERTGVVVEPRLSTQWFVKMAPLAKKAIDNQETDQAVEFFPPRFN
                     QTFLRWMENVHDWVISRQLWWGHQIPAWYHKETGEMYVGMEAPADSENWVQDPDVLDT
                     WFSSALWPFSTMGWPDTESEDYKRYFPTSTLVTGYDIIFFWVSRMIFQSLEFTGERPF
                     ENVLIHGLIRDEQGRKMSKSLGNGIDPMEVIEKYGADALRWFLSNGSAPGQDVRFSYE
                     KMDASWNFINKIWNASRFVIMNIEGMTVEEIDLSGEKSVADRWILTRLNETIERVTEL
                     FDRFEFGEAGRQLYNFIWDDFCDWYIEMSKEVLYGDDAVSKQTTRSILVHTLDQILRL
                     LHPIMPFVTEEIWEHIPHQGNSLVVAEYPVVRPEFDDETASKGMEVLKELIRAVRNIR
                     SEVNTPLSKPITLLIKTNDAKIDQFLVENTSYIERFCNPEELTISSEITAPDLAMSAV
                     LTGAEIFLPLAGLINIEEEIKRLEKELEKWTSEVKRVQGKLSNERFVSNAPEEVVEAE
                     RAKEKDYLEKQTAVKERIESLRTIQ"
     gene            18123..19436
                     /locus_tag="SCJ86_001807"
     CDS             18123..19436
                     /locus_tag="SCJ86_001807"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339588.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional folylpolyglutamate
                     synthase/dihydrofolate synthase"
                     /protein_id="HEG1169346.1"
                     /translation="MTIEEAIEWIHSRLPFGSRPGLDRVEALLEVVGHPERKVKTIHI
                     AGTNGKGSTVAYLRSLLEEAGLTVGTFTSPYIETFNERIAINGKSIPDNDLIQLVKKY
                     QPLIKQLDQFEAVAGITEFETLTAMAFDYFLMKKVDVAIIEVGLGGLLDSTNVAVPML
                     TGITTIGLDHTEILGDTIEEIAAQKAGIIKQGVPVVTGNIAPEAFTVIQQTAKAKNAP
                     LYVYGENYKINYLHPDEMWGEVFEFFNEIGKIPQLTTPLLGQHQAENAGVAIELYFVY
                     CQLEKLPFREKDVLKGLKKSRWPGRMERLSNEPLVVLDGAHNTHAMNRLVENLKKEFK
                     GYQINILFSALRTKNVREMLERLQQVPNVHIYLTTFEHPKAIELSEVSDLASERVSIV
                     SLWQFGLADILEKMTNEDVLVVTGSLYFISEVRHLLIQLGGDHEV"
     gene            19426..20079
                     /locus_tag="SCJ86_001808"
     CDS             19426..20079
                     /locus_tag="SCJ86_001808"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339589.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family phosphatase"
                     /protein_id="HEG1169347.1"
                     /translation="MKYNAVIFDMDGLLFDTELVYYEATQVVADKMGFPYDKDLYLKF
                     LGVSDEEVWANYHMIFSEFGKTSVQRFIDDSYQETLERFSQGAVQMKPGVLELLSFLE
                     EHQIPKVVASSNQRAVIELLLKKNNLFERFETIVSAENVQRAKPDPEIFLLANQKLGT
                     AKKETLILEDSQNGILAASAAEIPVVMVPDLLPPSQELIKKTAGVVSSLHEIPALLK"
     gene            20134..20685
                     /gene="radC"
                     /locus_tag="SCJ86_001809"
                     /pseudo
     CDS             20134..20685
                     /gene="radC"
                     /locus_tag="SCJ86_001809"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326507.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RadC"
BASE COUNT         7067 a         3474 c         4354 g         6010 t
ORIGIN      
        1 ctacactctg agatgtaaga aatatcataa gatatttctt acatcttttt gttttgagca
       61 ggactttaga taattttatt aatatatgct agcatgcttt tgtaagaaaa agaaagaggg
      121 cgtattagtt atgtatctta aaattacgga acaagcacaa gaaagattac aaaaatatat
      181 tgatgaaggc gcaacagtta tattggattt agatgatggc gttggggaat attctaaaat
      241 gggtgtttgc tctttagata ctagttttcg tttacttctt cttgataaag aacagagaaa
      301 aaatgattat aaactccaat tagatagtga tattggggat gtttatataa aagattattc
      361 aaaaatgtac atggatgaac atatgaccct ttctttagat ccacgtctag gtgtatttac
      421 tcttgaaagc ccaagtggtt ctttagacag tcatgttcaa ttagtagatc tacgaccaga
      481 aaaattctct ggaataaaac aataactttc tctctattgt ccataaaact agtctcaatc
      541 aagctgtatt gaaaaaacaa gacaacattc ttaccatggt aaataaaaag acgacacatt
      601 ttgtcgtctt tttttaaatt aactattttc gataaaagat cgcatagctt gtgccattat
      661 ttctggcttt tctctattca attcatgttt tccgttttta ataaagagta gccggccatt
      721 cgttgaaagt ttatataaac gttttgctgc tggtttattc gcatgatctt tggtaccgca
      781 taaaatcaat ttaggaagtc gtgatgtacg aagctgtttt ttgcagtcta atgtttcaag
      841 tgaacgaagt agggtaaggg tttgtttttt agatagacct aatttttcaa atatcttaga
      901 aggtacgcag taaaaataaa tattctggaa agtaagcaat aagcgattag gagactcaaa
      961 ttgtggagcg actaaaacaa ccttttttaa atatggagaa ggagttgcaa gatagtttaa
     1021 agcaataaca gcgcctaaag ataatccaca taaaataaaa ggttccttta tctgattcaa
     1081 ttgtacttcg atgtatttgg ttaagtcgtc taaagtagca gtgtctgtgc tgtttaaatg
     1141 attgaacaat gaaagacttt tgcaagaata actattgggt agataagaga ttacatcttt
     1201 ccatgattgt tcgtcttggc ctagaccatg caaaaaaaca attttcatat tgctctcctt
     1261 tttatagtta tcatatagac tttctttgta ctattaaaat ataaacattc atttcttacg
     1321 tcataaaata aaaaaacagc gcagacacat tgttaaacca acatgttgca ctgtttaagg
     1381 aagccacttg ccgggcttga accggcgacc tcttccttac catggaagcg ctctaccagc
     1441 tgagctaaag cggcattatc attaatacaa ataataggat aacgaatatt aatgtatttg
     1501 tcaacagaat tttttcgaaa aaatgtgttt ctctaatagg aaaacagatt ttagaaaaaa
     1561 taaaaataac aaaaaatgcg aacggtttct ttgataatat aataaaaaaa ttcaaaaaaa
     1621 gtattgcgtt atttagaaga aattgatatt atattcatgt acttgattcc ggcatagctc
     1681 agttggtagt agcgcatgac tgttaatcat gatgtcgtag gttcgagtcc tactgccgga
     1741 gtaaataaag cttatgcaaa gaaaaagaaa tccaatcact tgggtttctt tttcttttat
     1801 tattatgatg aatcaagctt tctgtttttg tcacgtatgt tgtaatcttt acgaaagagt
     1861 agagttggct actcataatc aaccatactt cccatcatcg ctaaatacga aataaatcag
     1921 cattcctaca ccaatcaaag cacaagctgc accaagaaaa gggatgacat tcccaaagtt
     1981 atctgtattc ataagtatgc caccacccac aaacaaaaag ccgccagcaa tcaggttttt
     2041 cattttattc atctcctttc ttacgttata gtatttcctt ctttcaatga gtattttgaa
     2101 agctatctta ttgtatgatg ttcttctagg cctcttcttt attatacagg ccattttcgt
     2161 tatctcatag aaatattcct ttttatctga aaactattgt ataccgtatg acatttttct
     2221 tcatgaagtt gtaagtatat tctcattcaa atgaaaattt catcatagaa gagattgtat
     2281 ttctaaattc ttaagagtaa aatacgaagt ataacttgga tttggaagga tgattggatg
     2341 aaagctatta cgcctgatat gacaacaaaa tttaacaaac agtttcaagc aaatatcaaa
     2401 cagcaggcat tgcagcgggc agttgtgaag aacggaatta ctgcttctgc tgaaaatgtt
     2461 tcagcacatg tggagaatac accggtattt tctatagatt tggctacggg gaaagtggct
     2521 aatcaaatgc aatcggggcg ttgctggatg tttgctgcac tgaacacatt ccgtcataag
     2581 atgctgaatc aattccattt aaaagagttt gaattatcac aaaattacac ttttttctgg
     2641 gataaatatg aaaaggcaaa ttatttctat gaaaacatca ttgcaacagg aaaagaggca
     2701 atcgatagcc ggaaagtagc tttcttactc gctactcctc aacaagatgg cggccaatgg
     2761 gatatgatcg tttcattatt ccaaaaatat ggcgttgtgc ctaaatcggt gatgccggaa
     2821 agtgcgaata gttcaaattc tcgagatttg aataactact tgaacaaatt actgcgtaaa
     2881 gatgcagtag tgttacggaa aatggttgct caaggagaaa cattagacgc tattgaagaa
     2941 aagaaagaag aaatgctaga aagcatttat aacttcttag ctatctcatt aggaacacct
     3001 ccaaaagaat tcgattttga gtaccgcgat gaagaaaaga actatcatct tgaccgagga
     3061 ctgacaccac aaatttttta tgataaatac atcggtgtaa aattagatga ttatgttagc
     3121 gtgatcaatg caccaacaaa agacaaacca ttcaatcgtt cttatacagt agagatgcta
     3181 ggaaatgtcg ttggcgggaa agaagtcaaa tatctgaatg ttgatatgga aactttcaaa
     3241 aaattagcga ttgctcagct tgaagaagga gaatctgttt ggtttggttg tgatgtaggt
     3301 caatcttcta ctagaactag tggcatcatg gccttagatg cttattcaat ggatgatcta
     3361 tttgatacgg attttacaat gactaaagct gaacgtttag attttggaga aagtctgatg
     3421 acccatgcga tggtattgac cggtgttgac ttagtagacg ggcaatcaac aaaatggaaa
     3481 gtcgaaaata gctggggcga aaaagttgga aagaatggct tctttgttat gagtgatgcc
     3541 tggatggacg aatatacgta ccaaatcgtt gtaagaaaag atttattgac gaaagagcaa
     3601 ttaaatgcgt tcaatgaaga accaattgtt ctatctccgt gggatccaat gggtgcttta
     3661 gcatagacga gtatttcaat gaagagtatg tagtaaagat aaaaagagct ggcttaagcc
     3721 agctcttttt atctttaaag tccattccct gttttttatg gtccctgtgc tataattcta
     3781 aataaaggat aaggattgga aataatcctt ttatcattaa aaatatgatg acgaagtaga
     3841 ataaaattgg gagtaggggg gaaagtcatg gatatccaaa ctgttttatc tgatctgaaa
     3901 ttaaatggta aagtgattcc agttgttggt ggcgatgtta atcagaccta tcgtataaaa
     3961 accgaacatc gtgcttattt tttaaaaatc catccgaatg taaaaaaagg attttttgaa
     4021 gcagaagtag acggattaaa agaactgtca gcattcgtac gtgtaccgga tacgtatatg
     4081 ctaggtgaaa caagtgaagg cgcttatttg ttaatggaat ggatcgagcc gggaaaaggg
     4141 gatcaacgag atcttgctgc agctttagct aatctccatc agcaaacagc gccacaattt
     4201 ggttttcgta aagataacta tttgggtaca cttgttcaga aaaattcttt tgaagaagat
     4261 tggtggactt ttttctttaa agatcggctt gaatcccaga tttctttggc agaagaaacg
     4321 aatcgatgga atgtacagcg tcaagaaaag tatctgcgat ttaaagaacg tgtattgaaa
     4381 tcggttgaac caaaaaagat cactccacgc ctcttgcacg gagacctgtg gagtggtaat
     4441 gtgttctttg accagcaagg gcatccagtt tttgtcgacc cagctgtttc ttatgggaat
     4501 cgggaacaag atattgcaat gagccagtta ttcggtggtt tccgaccgga atttttagat
     4561 gcttatcaaa ctatatttcc attagaaaag ggatggaaag accgactgcc gatttaccag
     4621 ttatattatc ttttggcaca tttgaatatg tttggagaaa gttacggttc ccaagtagat
     4681 caattactag aaaattttta agaggagggc gtgtttttgg caaaaaaata taccaaaaag
     4741 catgaagttt catattatga gtgtgacatc aaccaaacga tgacatttcc ttcgatgtta
     4801 ggagtagtta tcaaaacgtc tgaagatcaa agcgaccagt tgggaagagg cagtgaattc
     4861 gtcaatagct ttggacttac ttgggtcatc acgaattatt ccatggagat taatcgtctc
     4921 cctagagtcg gtgaagtaat ctcagtaacg actcaagcca tggagtacaa taagtttttc
     4981 tgttatcgga acttctggat ccatgatgaa gatgggaaag aattagtcaa aatagaatct
     5041 gttttcgtat tgatggattt tgtgaatcga aaaatgagca gtgttaatga agagatcatt
     5101 gctccttttg agagtgagaa gatcaagaaa atcaagcgtc aggaaaaaat tgaaaagatt
     5161 gattcgggct tcatgttgcc atatcgtgtt cgtttttatg atatcgatag taatcagcat
     5221 gttaataatg cgatgtattt taattggatc attgacgtat taggatatga ctttttgact
     5281 acgcatgttc cagaaaaagt aattattcga tttgataaag aagtagagta cgggaatgag
     5341 atcgaaagcc attttgaaca aatccctatc gaaaatggtg tcaaaacaaa acatgaaatc
     5401 cgaatgcaag aacagctgta ttgtgaagca aatattatat ggaaaaataa ataaaaaaga
     5461 ggttgtgaca gaagggggca acttcaagaa ataagaagaa atttacgaaa attgtttctc
     5521 aaatttttgt aaattttcga cttagttttc gaagaagcta tttttgtcac agcctctctt
     5581 tttaagttta gctattagat aatgatgttt agttttggat aaacgaaatc aattcttcac
     5641 ccgacaaacc atagtttaca gcaatcaata gttttaaacg tgctttttga ctattaatac
     5701 tgttacagaa aataatcccc attttttcta gttcttttcc cccgccttca taatcataaa
     5761 caggctctgc gattccattg aaacaacgag aaacaagtac tacagggatt ttttggacaa
     5821 gaagcttttc caaagcagct aaggcttgtg gagggagatt ccccgcacct aaagcttcga
     5881 ttaccaaacc gtctactttc gtatgggcga tggcttccaa taagtcccct tgcatcccag
     5941 catatgcttt aacgatcggg atagtcccat ctacagcgga aatgtccagt cgctttgttt
     6001 ctaaaagttc ctgcaagaat aaaattcgat tcttagttac taagccgatg ggacccaatg
     6061 taggtgttcg gaaagtagca acatttgtgg tatgggtctt tgtcacgtag cgtgcagaat
     6121 ggatctcatc gttcatgacc accaaaaccc ccttatctag cgcttcttca caacttgcga
     6181 cacgaatcgc actttcaaaa ttataaaggc cgtcacttcc aagttcatta cttgaacgca
     6241 ttgccccagt caaaacaatt ggtaatttgt tgccaatcgt tgtatcaaga aaaaaagcag
     6301 tttcttctaa tgtatctgta ccgtgtgtga tcaccactcc ggatatacct gatgcataag
     6361 cggacaagat acgttctttt aataccaaca tgtgagaagg catgatgtgt ggtgagggaa
     6421 gattgaagat atcttcaact aataattcta catgcttggg gattgtgatt tctgcatcta
     6481 ataatgggtt tgccacatca ggactaacac cgcccgtgag catgttttct ttcatggcga
     6541 tcgttccgcc ggtgtgtagt acaagtattt ttttcatttt acagacttcc ttacgaataa
     6601 ttttgcatag ccgttaaatc ttattgtata ctatataaaa gagttttgct atcagtgaag
     6661 aataacgaat agcctaactt atcataaaaa agctgacctt attaaaatgg aaaagactca
     6721 accttgacct cctaatacaa tggagtatct ggttgagttt cttttacaag aaagcagcca
     6781 aggaaatcaa tcacgaaggt ggccggaaat gatcagaatc ggattaacat cgtttaaaga
     6841 acatgacaaa ttaactggta aaaagaataa cactctttat gaatatgcag ggtttctacc
     6901 cttagtggaa ttagatactg cctattatgg aattccccga aagtcctcag ttgagaattg
     6961 gataagtgaa gtacctgaat catttcgctt tgtagtcaaa gtctatcaag gaatcagcgg
     7021 tcaaggagac tggaagcctt attatcagac cgaagaagag atgattgcgc attttttaga
     7081 tgttatgtcg ccaatggtag atgctggaaa gatgttttgc tttttagtcc aattcccctc
     7141 atcattcaaa tgtacgaaag aaaatgtcca gtacttgcgc aaacttagaa agtggtttga
     7201 gggttatcct gtttctatag aattgcggga ttattcttgg tacagcgatg aatttcttga
     7261 acgtacaaga aactttatgt ctagagccgc tttttcttta gccatcatcg atgagccaca
     7321 gttgctgaat acaacgattc cttttgatcc atttgtcacc aatccgaact ttgctctttt
     7381 tcgattccat ggtaggaatg cgcaaggatg gcaagcaaat gataaagagt ggcgtaaaaa
     7441 aagaacgtta tatcgttatt cagaaaaaga actgcaagaa ctagcacatg aagtcaaagt
     7501 agttgattct aagacaaaag aggtaggtgt gatttttaat aataattctg gtggtgatgc
     7561 ggctgaaaat gcattagcac tgcaaaaaat attaaatatc aagccagatg gactcaatcc
     7621 aacccaaatg gatctgtttt aagggcttat tttattcaag aaaggggcga agtctatgat
     7681 caaagctatt ttttttgata ttgacggaac attggtcacc aatcgttcaa aagcgttgga
     7741 atcaacaaaa caagcaattg aatatgctcg gaaaaacggc attttatgcg gtgtagcaac
     7801 ggggcgcagc ccagtaaaga tcaaagaaat cattgatgaa ttggaattag atatgtatgt
     7861 cgtgtataat ggtcaactgg tttttactgc agatcgcacg attatcgacc acccttttga
     7921 acaaaaagta ttggaacata ttgttgaatt tgcagatgag aaccatcgac aaatcgtttt
     7981 tggtgcgaga aatcggttag atggatcaac gacgatgcta ttaggacaat cgatttttat
     8041 caagcgacta gtcagctttt taccgcgtaa attcccggta cgtctgatga aaaaaatcct
     8101 ccaggttttt agccctcacc gacaaaagga tcgttatgaa acgttagaga tattaaaaga
     8161 accaatttat caatgtatat tgctcagtcc ggagtcagaa cagaaaaagt tagaacatcg
     8221 ctttcccgag tgtacgtttc agcgttcaaa tacgtatact gtagatatca tcccaaaagg
     8281 tggttcaaaa ttactaggca tccaagcttt cgcaaatgct gtcgaaattg aaatggaaga
     8341 aattatggca ttcggcgatc attacaatga tatcgagatg ctgaaaggtg taggaattgg
     8401 agtggccatg gggaatgctc agatcgaagt aaaacatgct gcagactttg tgactcaatc
     8461 aaatgaagaa gatgggatat acgaaggact gaaacacttc ggtctgattt attagtcaat
     8521 gaaagaagga aaaaatcagt atggaagcag agacaccttt tcaaaaagta gaaacattcc
     8581 acaaaacttt tgatccaaga agaccagaga tacccacggc ttttacagaa aatgaagctc
     8641 atacacgtgc tgcgttcaaa atagaagaac ttgttgaatt tgttgcagct acttcaccta
     8701 ataaagaaca actgcatgaa gctgttatgc tgctgcacga agcattagat aaagcagaag
     8761 aaaaaatact tggtaaaaca cagtggggaa gtgatccgct ggttgaacaa gtagatgcac
     8821 tgacagatat cctttacttt acttatggtt ctttttcatt actaggaata gtgcctgatc
     8881 gaatattcga aatcgtccat caggcaaata tggggaaatt atttccagat ggaaaacctc
     8941 attatgaccc tgtaacaaat aaagtgttga aacctgaaaa ctggcagatg gaatatgctc
     9001 cagaacctaa aatcgcggca gaaatccaac gtcagattca agaagcgatt gctaagaaga
     9061 aatccgctga tataggcatg tgcacaaatg aaaaagagtg atcagagttt cactattttt
     9121 gttatcttta tacgatggaa aaacaaaaga ttttcgttta agttagtgaa tgcatgaaaa
     9181 tagtatgcta taatctagaa tagatgtctt ttgcacgaga gacgtattat ttggatttat
     9241 gccaagcaga acgccatctg ttgcataaaa gatgcatact gaaaacggag gaaccataaa
     9301 tgggaaataa tgttatcatc ggaatcgtca ttgcaatcat tatagtcgcc attgtcgtct
     9361 ttttggttag ttacttgatg aagaaaaaaa accaagatcg attggatgcc ttagaaaaac
     9421 gcaaggaaga attatttgac ttgccagtga ttgaagaagt cgatgatgtt aaaaaaatgc
     9481 atctggttgg gcaaagccaa aatacctttc gtgaatggag tcaaaaatgg aatgacatat
     9541 ctacttcctc gttcgctgaa ctagaaagtc aaattttcga agtggaaaac ctcaatgaaa
     9601 gttttcggtt cttaaaagga aaaaaagcag ttgttgaagc agaagctaca atggaccaaa
     9661 tggagaaaca agtgacggaa atccgtgaag gtctaaagga attgcgggaa acggaagaac
     9721 gcaactcact agctgttcag caggcacttg acgtctatga agaactgaaa gaaaaagtca
     9781 gagaagacga gcatgaatac ggaccagctc tttttgaatt gcaaaaacag atcaaagtga
     9841 tcgaaaaaga atttacgcag tttgtgactt taaacacttc tggagaccct gttgaagccc
     9901 gcgaggtttt ggataaagca gaaaaacata cgtatgaagt ggaagctctg atgaaaaaaa
     9961 ttccaccgct gtatgaggag ctccatacca ctttccctga acaacttgaa gaaatcagtg
    10021 atacttacga gaaattgatg gaagaacaat atgtattccc agaagaagat cttgctgaag
    10081 atatcgcaaa agtaagtcga agaattgaaa attcattagc caatcttgaa aaaacagaag
    10141 ttgaaacggt cgaatttgaa aaccgtgaaa cagctgattt gattgattcg ttatatgata
    10201 tcttagaacg ggaaatggaa gctcagagat atgtaaaaac taaccaatca acgattgcag
    10261 agtatatcaa acatacaaca aagaataacc gtcaattatt gattgagctg gatcacacat
    10321 ctcaaagcta tacattgaac cacaatgaaa ttggccgtgt acgaggtttc caaaccgaag
    10381 tggaagaaat ggaacgtcaa aatgaacaga tgattccaca gatccgtcaa cacgaaattc
    10441 cttattcaga agtgcgcacg ttctataaaa cagtcttcaa agtcttagaa gatatcgaaa
    10501 agcaacaagt agagatcgac gactcattac atgaattgag aaaaggcgaa aaagaagcgc
    10561 aagaaaagat cgatacattc gaattcaaat tgcgcagttt gaaacgtttt gtagaaaaac
    10621 aacgtttgcc tggcttgccg aatgattacc tagaattctt ttttgttgct actgaccgta
    10681 ttgaagaact aagtgttgtc ttgaataaaa ttcgagtaaa catggaagag gtcaatcgtt
    10741 tagttgctct atgtgaagaa gatctagaat tattagataa gaagacacat gatttagtag
    10801 atgcagcagc attaacggag caaatgctcc aatatgcaaa tcgttatcgt catacacacg
    10861 aagaagtgag agaagcaatt gataaaagct actatttatt caataaagaa tatcactatc
    10921 aagaagcatt agacgaaatc ggtacagcat tagagcgagt agaacctggt gctttcaaac
    10981 gcatcgaaga tttttatttt aatcatccag acttagtata aagcaaaaaa gttagtaaac
    11041 cagcaagagc aagacggatt attgtcttgt tcttgtcttt ttttgtatat ttgaaaattg
    11101 aaacaagtga aaatttcacc tgctcttgaa agcaccttgg acaaccgtta taatagaaaa
    11161 gaatttcaaa aaagaggggt ttcttatgat atattttgat aacagtgcaa caaccccaat
    11221 ctttccgcaa agcttagatg catatgtaaa aaccagtcag cggattattg ggaatccatc
    11281 aagcttgcat gatttaggca atcaagctaa ccggttgttg cagcaagcaa gaaagcagat
    11341 cgctgatttg atccatgtag aaccagaaga aatctatttt acttcaggcg gaacagaagg
    11401 agataactgg gtattaaaag gaacgatgat cgaaaaaaga gaatatggga atcatatgat
    11461 catttcagga gtcgaacatc cggccgtcag cgagacagca gaacaattaa aagaattagg
    11521 aatcgaagta agtatcgccc cagtggataa acgcggattt gtacgtgtcg acgaattgaa
    11581 agaattaata agaaaagaga cagtattagt ctctgttatg gctgtgaata atgaagtagg
    11641 atcgattcag cccatccagg aaatcagtga tcttttggat gattttccta aaattcattt
    11701 ccatgtagat gctgttcagg cgattggaaa agtaccattc tcgcaatggt tgacagaaag
    11761 agttgatttt gcaacatttt cagctcataa attccatggc ccacggggaa ctggtttcat
    11821 ttattggaaa aagggtcgtc gtttagcacc attactgaca ggtggagggc aagaaagaaa
    11881 tcagcgaagt gggacagaaa atgttccagg tattgtggct atggttaaag ctttacgttt
    11941 atcattagag ataaaggaac aaaaacctga gcagacacaa cgcttaaaag tagctctaat
    12001 caatgcgttg aagacgtatg agaaagtgac gattttttca gaaggaactg actatgcacc
    12061 gcatatttta tgttttgctc taaaaggaat tcgcggcgaa gttctcgtcc atgccttaga
    12121 agaaaaacaa atatttgttt ctactactag tgcctgctcc tcacgtaaac atatggcaag
    12181 cagtacattg cacgcaatgc atgtaccaaa tgatctggct acatctgctg ttcgaatcag
    12241 cttagatgaa aacaattcgc ttgcagaagt cgagcaattt atgattattt tcaagcagct
    12301 ttataagaaa ttttctgcaa tcaattaaaa gaggagtgtt cttgtgaaat acagtgaaat
    12361 tatggtccgt tatggggaac tttcgacaaa aggaaagaat cgaaaaagct ttatcatgca
    12421 actagcacaa aatgtcagac aagctttgaa agactttcca gaaatcaaaa tccatgctga
    12481 tcgggaccgg atgcatcttt tattgaatgg cgcagacggg cagctagtta ttccaaaact
    12541 agcaaaaata tttgggatcc aaaatttctc tccaagtatt cgtgtagaaa aagatgtcga
    12601 agttttgaaa caagctgttc aagacatcat gaaagaaatt tatactggga aagaaacatt
    12661 taaaattatc gctaaaagaa gcgatcacca atttgaattg acaagtaatg aattgaatca
    12721 aacattaggt aatgctgttt ttgatatttt cccgcatatc caagttcaaa tgcgtcaacc
    12781 agatattcca ttacgtgtag agattcggcg tgatggcgct tatctatcgt atgaaacgat
    12841 caagggagct ggaggattgc ccgtaggaac aagcggaaaa ggaatgctta tgctttctgg
    12901 ggggattgat tctcctgtag cagggtactt tgccatgaag cggggagtgg aaattgaagc
    12961 tgttcatttt gctagtccgc cgtatacaag tgaacaagca ctgcaaaaag caaaagatct
    13021 aacagccaaa ctagcacctt atgtgggaac gatccatttt atcgaagtgc catttaccga
    13081 gattcaagaa gaaatcaaaa agtcagtgcc acaaggatat tggatgacaa ttactcgtcg
    13141 aatgatgttg cgtttgaccg atgaaattcg ccgtattcgt catggtttag tcattttgaa
    13201 tggtgaatca ttaggtcaag tggcttcaca aactttgcaa agtatggtgg ctatcaatga
    13261 agtaacgaat acgccaatca ttcggcctgt agcgacaatg gataaacttg aaatcattga
    13321 agtagcagaa cagatcgata catttaatct ttctatccag ccttttgaag attgctgtac
    13381 gatttttgct cctccacagc caaaaacacg ccctcaactg gaaaaggtcc atcaatatga
    13441 agagcgttta gcaatagatg aaatgatcca acgagcacta gctggcttga agatggaaga
    13501 gatacaagca gaatcgtcta atgaaaaaac tgaagaattt tcagaattgc tataagaggt
    13561 tatgaaaata gtgttttgac cagagtatta gagaaacaaa acgaaaaaat agctccatgt
    13621 attttaccgt ttttgaacta atgtcgaaga ttagctgatt gaataccatt tatcaagaag
    13681 ttatggaaaa gccggttctt ttttactaac tgaaaaataa aattttattt agagatcaag
    13741 ctacagttga taaatgatat aaaaaagaga atttcccatg aaatagcgag gttgtgacag
    13801 aagtgggcag cttcaagaaa taagaaggaa tttacgaaaa ttgcttttca aatttttgtg
    13861 aaatttcggc ttatttttcg aagaagttac ttctggaaca ccgtgtatca gaggttgcga
    13921 aaaagctgcc ctgcatcaag taataagaac agccaaacaa aaatagttcc tcatatttta
    13981 cgtttggttg agcttattac cgcagatgca agcttttgaa caccgtttta tttgtctcac
    14041 acccttttcc ttcctacttg taaccttgct cccgtgttag aaacatgtta cactaaatat
    14101 gtgaaattat taacaaggag cgtcgttatg cgagaaaaaa cagatacact gccaaaagca
    14161 actgcgaaaa gattaccttt atatttacgc tacttgaaga tgctggatga ctcaggtata
    14221 agtagaatca aatcaaatga gttcagcgaa atcacccaaa ttccttctgc aacgatccgc
    14281 agagacttct cgcaattagg cgagctaggc agaagcggat atggctatga tgtccctttt
    14341 cttatcgatg tattcaataa tatccttaat actaaagaag aaaaacgaat cgcattagta
    14401 ggatatggaa atcttggcaa agcattgaaa cacaacaatt ttagaagaaa tgaaaatcta
    14461 aatatcgtgt gcgtctttga taatgatcca gcactgatca atcgagtaat tgatggagaa
    14521 atgatctatc cgattgatcg tttcgcggag atagccaaag caaaaaatgt gacagtagca
    14581 atctctacgg taccaagtaa atattcacaa tctgctattg atgaaattgt caaaggaaat
    14641 gtaaccgcta tacttaattt tgcgcctgac agagtaactg tcccagctta tgtcaatgtt
    14701 caatacattg atttgacaac cgagttgcag acgcttattt actttgatga gaattactca
    14761 gaagtttttt cttaggaaaa tttgagtagg cttattgatc ttgcgagaac tcgtcgtaat
    14821 tctttcaaaa attttttagt actaaatgaa gtggtttttt cacgaccact tgttgactaa
    14881 aaaaaaaaac cattgtagaa tgaagcataa cgctgaacaa gaggagtaac tggcacattc
    14941 attcttagag agagaactat ctgctgaaag gttctcaatg acaaccagtg aaggtagctt
    15001 gggagtttct gtcttgaatg gagtagagat agacgagtcg tgatcgttaa ctcacttgag
    15061 aggcaataat gattgattat tgcaatttag gtggtaccgc gtaaatcata cgtcctagaa
    15121 tacgaaagta ttttaggacg ttttttagtt taaaggagga aaacagcatg acggaagaaa
    15181 ataacctatc aacaaaatat aatccgcaag aagttgaagc tggacgttat cagcattggt
    15241 tagaacaaga tttgtttaaa ccgagcggag ataaaaaagc aaaaccttat tcaatcgtaa
    15301 tcccgccccc aaatgttact ggaaaacttc atttagggca tgcttgggat acgaccttgc
    15361 aagatatgct gattcgtcaa aagagaatgc aaggatatga tactttatgg ctgcctggta
    15421 tggaccatgc aggaatcgcg acacaagcaa aagtagaagc gaagcttaga gagcaaggga
    15481 tttctcgtta tgatctaggt cgtgaaaagt tcatcgatca agtgtgggaa tggaaagaag
    15541 agtatgcgag ccacatccgc gaacagtggg ctaaaatggg cttatcttta gattacagta
    15601 gggaacgttt tacattagat gatggattat ctgaagcagt acgtaaagtt tttgttacct
    15661 tatacgaaaa aggtttgatt tatcgtggtg aatatatcat caactgggat cctcaagcac
    15721 gaacagctct atctgatatc gaagttattc ataaagatat cgaaggcgcc ttttatcata
    15781 tgagctatga actagcagat ggatcaggcg tagtagaaat tgcgacaacc cgtccagaaa
    15841 cgatgttggg agatacggcg attgctgttc atccagaaga cgagcgttat caagcgctca
    15901 tcggcaaaaa agtgattcta cctttagtca ataaacaaat cccaatcatt gcagatgaat
    15961 atgtagatat ggaattcggg acaggtgtag taaaaatcac tccagcacat gatccaaacg
    16021 actttgaggt aggtaatcgt catgatcttc cacgcgtaaa tgttatggat gatgatggta
    16081 cgatgaatga actagcagga aaatatgctg gaatggaacg ttttgctgct cgtaaagcaa
    16141 tcgtgcaaga tctgaaagaa atcggtcgat tgattaaaat cgatacgatg atccacagtg
    16201 tgggtcattc cgagcggaca ggtgtcgttg ttgaacctcg gctgtctact caatggtttg
    16261 taaaaatggc gccgttagcg aaaaaagcca ttgataacca agaaacagat caagcagtgg
    16321 aattcttccc tccaagattc aatcaaacgt ttttacgttg gatggaaaat gtccatgatt
    16381 gggtaatctc acgacaatta tggtggggac atcaaattcc tgcatggtat cacaaagaaa
    16441 caggtgaaat gtatgtaggg atggaagcac cagcggatag tgaaaactgg gtacaagatc
    16501 ctgatgtttt agacacttgg tttagttcag ctttgtggcc tttctcaaca atgggctggc
    16561 cagatacgga aagcgaagat tataagcgat acttccctac tagcacttta gtaaccggtt
    16621 atgacatcat tttcttctgg gtaagcagaa tgattttcca aagcttggaa tttactggcg
    16681 aacgtccatt tgagaatgtc ttgattcatg gattgattcg tgacgaacaa gggcggaaga
    16741 tgagtaaatc attaggtaat ggaatcgatc cgatggaagt aatcgaaaag tatggagcag
    16801 atgctctccg ctggttctta tcgaatggtt ctgcaccagg acaagatgtc cgcttcagct
    16861 atgaaaaaat ggatgcttct tggaacttta tcaacaaaat ctggaatgct tcacgtttcg
    16921 ttatcatgaa tatcgaagga atgaccgtag aagagatcga tttatctgga gaaaaatcag
    16981 tggctgaccg gtggatcctt actcgtttga atgaaacgat cgaacgagta actgagctat
    17041 ttgatcgttt tgaatttggt gaagcaggtc gacaactgta taactttatc tgggatgatt
    17101 tttgtgactg gtacatcgaa atgagtaaag aagtacttta tggagatgat gctgtttcaa
    17161 aacaaaccac ccgtagcatc ttagtccata cacttgatca gatcctacgt ttattgcatc
    17221 caatcatgcc attcgttaca gaagaaatat gggaacatat tcctcatcag ggaaattctc
    17281 tagtagtggc agaatatcct gttgtacgtc cagagtttga tgatgaaaca gcttctaaag
    17341 ggatggaagt attgaaagaa ttgattcgag ccgttcgaaa tattcgttca gaagtgaata
    17401 caccattgtc taaaccaatt acgttgctaa ttaaaacaaa cgatgctaaa atcgatcaat
    17461 ttttagttga aaatacaagc tatatcgaac gcttctgtaa tcccgaagag ttgacgatat
    17521 caagtgagat cactgcgcca gatctagcga tgtcagctgt tctgacaggt gcagaaatct
    17581 tcttgccgtt agcaggattg atcaatattg aagaagaaat caaacgatta gaaaaagaac
    17641 tggaaaagtg gacaagcgaa gtaaaacgtg tccaaggaaa actttctaat gaacgttttg
    17701 tttctaacgc gccggaagaa gtagtagagg cagagcgtgc aaaagaaaaa gattatttag
    17761 agaagcaaac ggctgtcaaa gaacgaatcg aaagtttgcg tactattcaa taagaataga
    17821 aaaagagagt gtgaaatcag ctagctatta ctaaaagcta gctgattgaa catcgttaca
    17881 ggaaagagtt tgagcctcac tcttagcttc gattcataag gagatgtgac ataaattttg
    17941 tcgcatctct tttttagtct actcttatta tgtaggagaa ggagggaggt ggatcatgta
    18001 tcaagagtca ataaagtagt tgataaaatc gttcctctct ctgtttcttt tctctttgtc
    18061 atacttttat ttttcttatg tgttctattt gttataataa aagagaaatg ggggaattgt
    18121 tgatgacgat tgaagaagca atagaatgga tccatagtcg attaccattt ggttcacgtc
    18181 ctggccttga ccgagtcgaa gctttattag aagtggtagg gcatccagag agaaaagtaa
    18241 aaacgatcca tatcgctgga acaaacggaa aaggttctac tgtagcttat ttgagaagcc
    18301 tattagaaga ggctgggctg acagtaggta catttacttc accttatatc gaaacgttca
    18361 atgaacggat cgcaatcaat ggcaaaagca ttccagacaa tgatttgatc cagcttgtaa
    18421 aaaaatatca gccactgatc aaacaacttg atcaatttga agcagtagct ggtattactg
    18481 aattcgagac attaacagca atggcatttg attatttttt gatgaagaaa gtggacgtag
    18541 cgattatcga agttggctta ggcggcttat tggattctac caatgtagct gtaccaatgc
    18601 tgacgggaat aacaacgatt ggacttgatc ataccgaaat acttggagat acaattgaag
    18661 aaatcgctgc acaaaaagct ggaatcatca aacaaggcgt tccagtagta acaggaaata
    18721 ttgccccaga agcttttact gtcatccaac aaacagctaa agcaaaaaat gcaccgcttt
    18781 atgtatacgg ggaaaattat aaaataaact atctgcatcc tgatgagatg tggggagaag
    18841 tatttgaatt ttttaatgaa ataggaaaga tccctcaatt aaccactccg cttttagggc
    18901 aacatcaagc tgaaaatgca ggagtagcca tcgaattgta ttttgtttac tgtcaattgg
    18961 aaaagctgcc tttccgagaa aaagatgttt tgaagggatt gaagaaatct cgatggccag
    19021 gaagaatgga gcgtttgagt aacgagccat tagttgtttt agatggtgcc cataatacgc
    19081 atgcaatgaa ccggcttgtt gagaatttga aaaaagaatt caaaggatat caaatcaaca
    19141 ttttgttttc cgccttacga actaaaaatg ttagagaaat gctagaacga ttgcagcaag
    19201 taccaaatgt ccatatttat ttgacgacct ttgaacatcc aaaagccatc gaattaagtg
    19261 aagtatcaga tttagcaagt gagagagtat caatcgtttc attgtggcaa ttcggattag
    19321 cagatatatt ggaaaagatg acaaatgaag acgtattagt tgtgaccggt tcactttatt
    19381 tcatttcaga agttcgccat ttattaatac aattaggagg agatcatgaa gtataatgca
    19441 gtgatttttg acatggatgg tttgctgttc gacacagagt tagtgtatta cgaagccaca
    19501 caagtagtag cagacaagat gggttttcct tatgataaag acctgtattt aaaattctta
    19561 ggtgtttcag atgaagaagt atgggcgaat tatcatatga ttttctcgga atttggcaaa
    19621 acaagtgtac aaagattcat agatgattca taccaggaaa cgcttgaacg tttttcgcaa
    19681 ggagcggtac aaatgaagcc cggtgtgttg gaactcctct cttttttaga agaacatcag
    19741 attcccaaag ttgtcgcttc cagtaaccaa cgagcagtca ttgaactttt attgaagaag
    19801 aacaatctgt ttgaacgatt tgagacgatt gtttctgcag aaaatgttca acgagcaaaa
    19861 ccagacccag aaattttctt gttagctaat cagaagctag gtacagcgaa gaaagaaaca
    19921 ttgattttag aagattcaca aaatggtatt ttagcagctt ctgcggctga gatcccggta
    19981 gtcatggtac cagatctgct tccaccatcc caagaattga tcaaaaaaac agctggggtt
    20041 gtttcttctt tacatgaaat ccctgcattg ttaaaataaa aatccatctc ttttcgtatc
    20101 ttattttaga taagatagaa aggagatgtt tttttgggaa aacgattgat caaagaagta
    20161 cctactagct ctttaccaag ggaacgtatg gaaatttatg gcgcggaagc tttatcggat
    20221 caggagcttt tggcaatctt actgcgtacc ggtcaacacc cttatagtgt gatgtcgatt
    20281 gctggaaatc tgttaaaaac gtttggagga ctggcctctt tacgacaggc tactttgcat
    20341 gagttagaag agattcgagg gataggacgc gtgaaagcaa tcgaaatcaa agccttgatc
    20401 gaattaggtc gacgaattca gacggatcat gaaagagcgg ctccaccggt tcgtaccagc
    20461 tacgaattgg cccagcaatt gattctggaa atgaaagatc acaaacaaga acatttggtt
    20521 tgtatttatt tggacacgaa gaatcaagtg atcttaaaaa aaacagtgtt catcggttcg
    20581 ctgaatcagt cagtcgctca tcctagagaa attttccatt atgctgtacg ttattgtgca
    20641 gcaaggatcg ttttagcgca taatcatcct tctggtaatg ttttaaccat agtataataa
    20701 ataatagtag ttaaagtagt ttaagaggag atattaaagg gatattttac tttaaataaa
    20761 aattaacaag gtttataatt tgtagtagtt aaagtattta aagaggtggg aacatgtgaa
    20821 ggttcaaagg atagaagtgg agaataagcc gtatccattg tatttattac tagataaaga
    20881 ataccagcta atagaaccag taatg
//
LOCUS       DARBET010000045        20485 bp    DNA     linear   BCT 07-NOV-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4027
            SAMEA114569841-rid20112183.denovo.045, whole genome shotgun
            sequence.
ACCESSION   DARBET010000045 DARBET010000000
VERSION     DARBET010000045.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA114569841
            Sequence Read Archive: ERR12203666
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 20485)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 20485)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001972618.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001972618.1
            Genome Coverage       :: 132x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/07/2023 13:18:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,041
            CDSs (total)                      :: 2,975
            Genes (coding)                    :: 2,908
            CDSs (with protein)               :: 2,908
            Genes (RNA)                       :: 66
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 67
            CDSs (without protein)            :: 67
            Pseudo Genes (ambiguous residues) :: 0 of 67
            Pseudo Genes (frameshifted)       :: 26 of 67
            Pseudo Genes (incomplete)         :: 28 of 67
            Pseudo Genes (internal stop)      :: 20 of 67
            Pseudo Genes (multiple problems)  :: 6 of 67
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20485
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA114569841-rid20112183.denovo.045"
                     /isolate="VRE4027"
                     /db_xref="taxon:1352"
                     /country="Denmark"
     gene            4..552
                     /gene="hslV"
                     /locus_tag="SCJ86_001810"
     CDS             4..552
                     /gene="hslV"
                     /locus_tag="SCJ86_001810"
                     /EC_number="3.4.25.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002357489.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HslU--HslV peptidase proteolytic subunit"
                     /protein_id="HEG1169348.1"
                     /translation="MVESPFHSTTICAVEKDGKFAMAGDGQVTMGESVVMKGTAKKVR
                     RIYNGEVVVGFAGSVADAFTLEEKFEGKLNEYNGNLQRAAVELAQEWRTQQSMQKLEA
                     MLIVMNDKEMLLVSGTGEVITPDDGILAIGSGGNFALAAARAMKNYAQNDMSAKEIAE
                     NALNIAADICIFTNHNIIVEEL"
     gene            566..1966
                     /gene="hslU"
                     /locus_tag="SCJ86_001811"
     CDS             566..1966
                     /gene="hslU"
                     /locus_tag="SCJ86_001811"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002409948.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent protease ATPase subunit HslU"
                     /protein_id="HEG1169349.1"
                     /translation="MYELKQTPRQIVSELDEYIVGQHAAKKSVAVALRNRYRRLQLDE
                     KMQQDITPKNMLMIGPTGVGKTEIARRLAKIVNAPFVKVEATKFTEVGYVGRDVESMV
                     RDLVENAIQIVEKEQYSRVYTSAKKNAEKRLVKILVPGIKKEQKQSTNQFEQMMNLFN
                     ASQQNTAEPQEEVTEDIRINRRTVLEQLQKGLLDSREVTIEVEDQKKPAPMMNNGLEQ
                     MGIDLNETLGALTPKKKVERTVTVKEALELLIKEESAKLVKDADIHSEAIKLAEESGI
                     IFIDEIDKITSKSQQSGEVSREGVQRDILPIVEGSQVNTKYGIIQTDHVLFIASGAFH
                     LSKPSDLIPELQGRFPIRVELDDLTAEDFVKILTEPNNALVKQYVALIGTENVSVTFT
                     KEAIERIAQIAFDVNRDTDNIGARRLHTILEKLLEDLLFEAPDMQMGEITITEAYVDD
                     KLGSIAANEDLSRYIL"
     gene            1984..2775
                     /gene="codY"
                     /locus_tag="SCJ86_001812"
     CDS             1984..2775
                     /gene="codY"
                     /locus_tag="SCJ86_001812"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016176812.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTP-sensing pleiotropic transcriptional
                     regulator CodY"
                     /protein_id="HEG1169350.1"
                     /translation="MTDLLTKTRKINTLLQQKNTFDMQADLPYDKMAVTLGGILDSNT
                     YIIGSDGVLLGYNERHDVNNERIKTMFVEKQFPESYTDTVAQLMKTEANIPITSDITA
                     FPVESREKYPFGLTTVVPIFGAGERLGTIILSRMEQSFADDDLVLAEYGATVVGMQIL
                     YQKSRSIEADVRSATAVQMAINTLSYSELKAVQAIFEALDGDEGRLTASNIADSIGIT
                     RSVIVNALRKLESAGIIESRSLGMKGTYLKVLNSRFKDELAKHSY"
     gene            2857..3732
                     /locus_tag="SCJ86_001813"
     CDS             2857..3732
                     /locus_tag="SCJ86_001813"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296994.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldose 1-epimerase family protein"
                     /protein_id="HEG1169351.1"
                     /translation="MTVIIQNDQLIAEIAEHGAELISLKSKEHGLEYIWQGDPAYWGR
                     HAPVLFPFVGRLKDDQYVYQGKTYQMGQHGFARDMDFEVIEHGQEMASFILRSTPETL
                     EKYPFDFELVISYELGGDGITVHYQVANTGEKEMFFSIGGHPAFNVPLEEGLSFEDFY
                     LAFSPLKSRLRLPLKGPFIDMDQKTLGQTNTNLALNRELFEQDALVFETRGLNAYAIR
                     SEKSSHSVTLSYKDMPYVGIWSPYPKEAPFVCIEPWCGIADTVNSSGNLMEKVGINQL
                     GAHEIFKTKYSITVK"
     gene            complement(3864..4502)
                     /gene="plsY"
                     /locus_tag="SCJ86_001814"
     CDS             complement(3864..4502)
                     /gene="plsY"
                     /locus_tag="SCJ86_001814"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002390767.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol-3-phosphate 1-O-acyltransferase PlsY"
                     /protein_id="HEG1169352.1"
                     /translation="MKLILLLILAYLLGSIPSGVWIGKLFFKKDIRQHGSGNTGTTNT
                     FRVLGKTAGIVVLLMDILKGTLATCLPMIFHLSGINPLWFGVCAILGHTFPIFAGFKG
                     GKAVATSAGMLLGFNPVFFVYSCIIFIVSLFCTSMVSLSSMISAVLITLSTIVLPYVA
                     PVILAKPNWLLTIIAFLVSSFIFYRHKDNIKRIRNHTESRIPFGLNSSKKKS"
     gene            4629..5063
                     /locus_tag="SCJ86_001815"
     CDS             4629..5063
                     /locus_tag="SCJ86_001815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330223.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CoA-binding protein"
                     /protein_id="HEG1169353.1"
                     /translation="MSVKNPTQETIFNYLEEAKNIAVVGLSNKPERTSYMVAQVLQDN
                     GYKIIPVNPLLAGEEILGEKVYANLTEIDDPIDIVDVFRRSEFLPEIAGDFLKTDAKV
                     FWAQLGIENKEAAEMLRKAGRTDIVMDRCIKIELGKLKENQK"
     gene            5229..7274
                     /gene="parE"
                     /locus_tag="SCJ86_001816"
     CDS             5229..7274
                     /gene="parE"
                     /locus_tag="SCJ86_001816"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331043.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase IV subunit B"
                     /protein_id="HEG1169354.1"
                     /translation="MAKKVNNEYNDSSIQVLEGLEAVRKRPGMYIGSTDSRGLHHLVY
                     EIVDNAVDEALSGYGSEIDVTIHEDNSITVADSGRGMPVGMHASGIPTVEVIFTVLHA
                     GGKFGQGGYKTSGGLHGVGASVVNALSKWLIVTIVRDGVEYQQKFKNGGKPDGTLKKI
                     GKTKKANGTTVHFLPDDTIFSTTKFSYEILAERLRESAFLLKGVKISLSDLRGEEPVK
                     EIFHYEEGIKEFVDYLNEEKDTLTPVVYFSGEKEGIEVEVAYQYNDGYSENVLSFVNN
                     VRTKDGGTHEAGMKAAMTKSYNEYARKVGLLKERDKNLEGSDFREGLAAVLSIRVPEN
                     LLQFEGQTKEKLGTPVARTVVDNVISEQMGFYLQENSEMSQMLVRKAIKAREAREAAR
                     KAREESRNGKKRKKGESLLSGKLTPAQSRNPKKNELYLVEGDSAGGSAKQGRDRKFQA
                     ILPLRGKVINTEKAKMQDILKNEEINTMIYTIGAGVGPEFSIEDCNYDKVIIMTDADT
                     DGAHIQVLLLTFFYRYMKPLIEAGKVYIALPPLYKVSKGQGKKQVIEYAWTDDELAAM
                     IKKVGKGYMLQRYKGLGEMNAEQLWETTMDPTSRTLIRVRIDDAAQAERRVTTLMGDK
                     VEPRRKWIENHVQFTLEEDGSILDKKEDTEISPSVSNDLLDEERADKNENNQLFEVE"
     gene            7286..9736
                     /gene="parC"
                     /locus_tag="SCJ86_001817"
     CDS             7286..9736
                     /gene="parC"
                     /locus_tag="SCJ86_001817"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298103.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase IV subunit A"
                     /protein_id="HEG1169355.1"
                     /translation="MENRQEVQELTLEEVMGDRFGRYSKYIIQERALPDIRDGLKPVQ
                     RRILFAMNKDGNTYDKGFRKSAKSVGNIMGNYHPHGDISIYEAMVRMSQDWKLREVLI
                     EMHGNNGSMDGDPPAAMRYTEARLSKLSGEMLADIEKETVDLVWNFDDTEKEPTVLPA
                     RYPNLLVNGSTGISAGYATEIPTHNLAEVIDGTIYMIDHPQASLEKLMEYIPGPDFPT
                     GGILQGKDEIKKAYETGRGKVILRSKTKIEAMKGNKQQIVITEIPYEVNKATLVKKMD
                     EIRLNKKIDGIAEVRDESDRTGLQIVVELKKDANAQGILNYLFKNTELQINYNFNMVA
                     IDHMTPHQVGLKDILRSYIEHRKQVITKRSQFDLAKAQKRQHIVEGLMKALSILDEVI
                     ATIRESKDKKDAKKNLVDVFQFTEEQAEAIVTLQLYRLTNTDITELQKESESLIAQIT
                     ELNKILSNDKELFSVMKKELREVKKNYSSARLTTIEDEIEEIKIDTQVLVAQEDVIVS
                     VTREGYVKRTSLRSYSASKPEEIGMREGDYLLYSGELSTLDHVLLITNKANVIYRPVH
                     ELPDLKWKDAGEHISQTISNLSVDESILAVFPYQKIQAEKTFVFISKNGLIKQTRMTE
                     FEPWRTYKSRPLSCMKLKAEEDELIAVYLKEDQPGLDVFLVTHQGMGLRYPLTDVPVV
                     GAKAAGVKSMNLKDGDRIINGLLVLAEGDTPIVIVTQRGAVKRMLAQEISQTSRAKRG
                     VTVMRELKKNPHRIIYMSEGRSKEITLMNQKGQQLTIDPTDFPIGDRTSNGSFAMDEK
                     KGGEVIEVIDAPEIFIES"
     gene            10013..12244
                     /gene="pflB"
                     /locus_tag="SCJ86_001818"
     CDS             10013..12244
                     /gene="pflB"
                     /locus_tag="SCJ86_001818"
                     /EC_number="2.3.1.54"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005878172.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate C-acetyltransferase"
                     /protein_id="HEG1169356.1"
                     /translation="MEQWNGFKGTKWKEGVDTRDFIQNNYTEYRGDDSFLEGPTEATE
                     KLWDELQKLNDIQFERNGVYDMDTEIVSTITSHDAGYLDKDLEKIVGLQTDKPLKQAF
                     MPFGGINMANNALKSNGYTPNDELTHIFTDWRKTHNQGVFDAYTAEMRAARKNKIITG
                     LPDAYGRGRIIGDYRRVALYGIDYLMEQKKKDHDMTGYHTMSEEVIRLREEITEQYRA
                     LAQMKEMAAKYGYDISKPAANAKEAVQWLYFGYLAAIKSQNGAAMSIGRISAFLDIYI
                     QRDLDNGVITEKEAQELIDHLVMKLRMVKFARTPEYNQLFSGFPIWATLSIAGMGLDG
                     RSLVTKNDFRLLHTLTNMGPSPEPNLTVLYSEHLPEGFRTYASRIAIESSSIQFENDN
                     QLREQWGSDDCAIACCVSGTVVGKDMQFFGARANLAKTLLYAINGGVDEVTGAQVGPE
                     YRPITSEILDFEEVKHNFMNMMDWLAELYVNTLNIIHYMHDKYSYEAAQLALMDSDLQ
                     RTFATGIAGISHAADSLSAIKYAKVKAIRNEKGIAVDFEVDGDFPKYGNDDDRADELV
                     SWILEYFMGQIKRHKTYRNSKPTTSLLTITSNVVYGKNTGNTPDGREAGKPLAPGANP
                     SYNAEQSGLLASLNSTAKMNYGCALDGISNTQTINPSGLGKEQDTRIENLRNVLDGYF
                     SKGAYHLNVNVFSNDLLRDAMENPMKYPNLTIRVSGYAVKFRDLTREQQLDVLMRTSH
                     DRM"
     gene            12488..13249
                     /gene="pflA"
                     /locus_tag="SCJ86_001819"
     CDS             12488..13249
                     /gene="pflA"
                     /locus_tag="SCJ86_001819"
                     /EC_number="1.97.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319416.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate formate lyase-activating protein"
                     /protein_id="HEG1169357.1"
                     /translation="MEEKTIGYVHSIETFGSVDGPGLRFVVFMQGCRMRCQFCHNPDT
                     WNIGGGKEYTADELLDKAERFRPYWGDKGGITVSGGEPLLQIDFLIELFKKAKERKMH
                     TTLDTCGKPFTYEEPFFSRFQELMKYTDLLLFDIKHIDNEEHKKLTHWDNENILEMAQ
                     YLSKINKPVWIRHVLVPERSDYDEYLIRLDNFIQTLSNVDRVEILPYHTMGKYKWETL
                     GLKYPLEGIEPPTKERVENAKRLLHTEEYTKYLTR"
     gene            13334..14260
                     /locus_tag="SCJ86_001820"
     CDS             13334..14260
                     /locus_tag="SCJ86_001820"
                     /EC_number="3.6.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="manganese-dependent inorganic pyrophosphatase"
                     /protein_id="HEG1169358.1"
                     /translation="MSKILVFGHQNPDTDAIGAAIAFSHLQNELGKETEAVALGTPNE
                     ETQYALDHFQLSAPRVVESAKAENADVMLVDHNEFQQSISDISEVNILAVVDHHRIAN
                     FETANPLYYRAEPVGCTSTIILKLYKENQVTIPKEIAGIMLSAIISDTLLFKSPTCTP
                     EDEAAAKELAAIAEVDLNTYGLEMLKAGTNLSDKSAAVLLDLDAKSFPMGSSNVRIAQ
                     VNTVDLNEVMDRQAELEAAMEDEMKANGYDLFVLVVTNILDSDSELLVAGKGKEKVEE
                     AFQTTLTNNRALLKGVVSRKKQVVPQLTAVFS"
     gene            14442..15335
                     /locus_tag="SCJ86_001821"
     CDS             14442..15335
                     /locus_tag="SCJ86_001821"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008264701.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1803 domain-containing protein"
                     /protein_id="HEG1169359.1"
                     /translation="MNYYSKNKENFYQKLTDDPLFSSLTDYLYEHREQETILRELKKE
                     FSQNKFSHFLDLLIDAGLIKREERRYHLNFPIFDPKDYLQQATSAAETIAEQLKRLSV
                     DEQKLAMGEVIWAYCFEDERKEAYFYGVRNSRETELLRTTAGNEKYRFITLSSIEHFP
                     LTLANYFFVQKNQLPVTKAFKELAELIGDVNESYFFDQIEVIVDRIRKNKYKNRRPSI
                     FHQSLLVTNTIKEEESFTLELPIVEKNNFEIELPTLDPSLTMEEMAFLKRQIFSELSK
                     KFIPHAFSYIKEYGTVLVSKT"
     gene            15469..15594
                     /locus_tag="SCJ86_001822"
     CDS             15469..15594
                     /locus_tag="SCJ86_001822"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEG1169360.1"
                     /translation="MQKNGEKCGMTKEVVIRKVRFLNNQYYDSVKYGILWEELAD"
     gene            complement(15728..16360)
                     /locus_tag="SCJ86_001823"
     CDS             complement(15728..16360)
                     /locus_tag="SCJ86_001823"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345061.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEG1169361.1"
                     /translation="MEIQRRTTNFLHLGSFFATLTILFFLCYSYLKTSHAPSSALGWL
                     LLDHSDITEAEENPFFLILSSLCGLLFSVYFGAYFFHRHPGTLNEASSVLIAFKTFSF
                     LAILFYSFNHNTLFFFIMNGLVVILSIFTYFIFSLIFSQLKCPLFFRLVPVTCFIYSL
                     FSFFVLALMPLTNPSTIQVAGNLLDMLFIVSLGVFMWQRKNKQNKKHFEA"
     gene            complement(16508..17050)
                     /locus_tag="SCJ86_001824"
     CDS             complement(16508..17050)
                     /locus_tag="SCJ86_001824"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300416.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2179 domain-containing protein"
                     /protein_id="HEG1169362.1"
                     /translation="MIDPKMLGMIFLINFAYITLNTLRFMLTMKGYRLIAPLVSMVEI
                     TIYIVGLSLVLDRLDNPLNLFVYALGYAVGISVGILIEDKLALGYIMVTTILPANSAE
                     DKSLPGILREHGYGVTQTSAMGLEGERLVLEILSPRKSERELYRLIKEIEERAFIISY
                     EPKYISGGFWTKKVRKRQGK"
     gene            17185..17574
                     /locus_tag="SCJ86_001825"
     CDS             17185..17574
                     /locus_tag="SCJ86_001825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342228.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEG1169363.1"
                     /translation="MKTVNRVFCASHVLSLLLSIYLGVYSIQQPIEVENIVFQMSLTD
                     GLILSVFFFLILFIGNIFGSVASFLNFSIYPLLSLALGVVGLCSLCLFPEPFSPAFIL
                     FGTLNLFQTTVGAWLLWRSGNLMKIGE"
     gene            17578..18342
                     /locus_tag="SCJ86_001826"
     CDS             17578..18342
                     /locus_tag="SCJ86_001826"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008264666.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HEG1169364.1"
                     /translation="MNPIILKRSVHHIPVLEVVLKELREERIPVVIYYHGWQTSKELV
                     LTQGRKIAQQGIRVILPDAMNHGERKQPVSSIPSYTFWDSIYGNLFEFDTLIKHLEKR
                     GLLHDQLAVGGVSMGGMTTCALLAKHPHIVAGVCLMGSPAPLEYGKEIVRRASEYNYV
                     VPKDYFDLISWVKSYDLSLQPEKLAGRPLFFWHGQADEKIPYIQTERFVEKNRHKSYG
                     KEIVFRSSKTQRHMVQIPLMEEASTFLAKNLIKKYK"
     gene            18471..19418
                     /locus_tag="SCJ86_001827"
     CDS             18471..19418
                     /locus_tag="SCJ86_001827"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HEG1169365.1"
                     /translation="MPTLADVAKRANVSKMTVSRVINHPEQVTDELKELVFSAMAELD
                     YRPNIAAKALVTNRSQIIKLFILEEIDTTEPYYMNLLMGIAKRIGKAHYSLQLVTNDA
                     FDVGSCDGYIITGVRKKDFDWIDRLEKPVVLFGENSEGYDYVDSDNEYGTAKATQYAL
                     ERDYQTIIYIGIEVDEPFEISRETGYLSVMKEVDLPSKILRFPNHSTEVERYMTENWA
                     DFTDKTCFVCSSDRLALGAVRGIINAGGHLPEEFGVIGFDGVFLDQISSPKLTTIKQD
                     IIRMGEVCGEMLLKKIEEKGASQGYRRFFPELVIRETTN"
     gene            19548..20288
                     /locus_tag="SCJ86_001828"
     CDS             19548..20288
                     /locus_tag="SCJ86_001828"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338209.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEG1169366.1"
                     /translation="MKEIRRKHFRFPAYDDELGVKLTNDKKRVLFDGQEDLLTEQNQS
                     PFEQMKEFHFTNTNTEKIRRKRRATNGLKSAPRKENLEKHKGKLPDYTGHYQSEITST
                     GRKKLFGDSSRSVRQSETRTESKHTTRNASVDTKVRERSYFVPKYVPASIIPDPRESK
                     ISEDHLIESMKKTDDSYLLFDTEPTPYQEKRNGEPSVRTFNRTKTLEETKQSEEKKSK
                     KSHGILERSLQGLIDDQGNSLGENSYFK"
BASE COUNT         7193 a         3481 c         4316 g         5495 t
ORIGIN      
        1 aacatggtcg aatcaccatt tcattcaacc actatctgtg ccgtcgaaaa agacgggaaa
       61 tttgctatgg ctggagatgg ccaagtaaca atgggtgaat ctgttgtcat gaaaggcact
      121 gcaaagaaag tacgacgtat ctacaatgga gaagttgttg taggtttcgc cggaagtgtt
      181 gcagatgcgt ttactttaga agaaaaattt gaaggcaaac taaacgaata taacgggaat
      241 ctacaacgcg cagctgtcga attagctcaa gaatggcgga cacagcaatc tatgcaaaaa
      301 cttgaagcga tgctgatcgt aatgaatgat aaagaaatgc ttcttgtttc aggtacagga
      361 gaagttatca caccagatga tggtatctta gctatcggat caggagggaa ttttgcgttg
      421 gctgcagccc gagcaatgaa aaactatgct caaaacgaca tgagtgcgaa agaaattgca
      481 gaaaatgcgt taaatattgc cgcagatatc tgtatcttta ctaatcataa tattatcgta
      541 gaagaactgt aagaggtgag tagatatgta tgaattaaaa caaacaccaa gacaaatcgt
      601 aagtgaatta gatgaatata tcgtaggaca gcacgcagca aaaaaatctg ttgccgttgc
      661 tttgcgtaac cgctatcgcc gtttgcagtt agatgaaaaa atgcaacaag atattacacc
      721 aaaaaatatg ctgatgatcg gacctacagg ggtcggtaaa acagaaatcg cacgacgttt
      781 ggcaaaaatc gtcaatgcac catttgtcaa agtcgaagca acaaaattta ctgaagtcgg
      841 ttatgtcgga agagatgtag aatcaatggt tcgggattta gtagaaaatg cgatccaaat
      901 cgtagaaaaa gagcagtaca gccgtgtata cacaagtgct aaaaaaaatg cagaaaaacg
      961 tttggtaaaa atcttggtac ctggtatcaa aaaagaacag aaacaatcca ctaatcaatt
     1021 tgaacaaatg atgaatcttt ttaatgctag tcagcaaaat accgctgaac cgcaagaaga
     1081 agtgacagaa gatattcgaa tcaatcgccg tacagtcttg gaacaactac aaaagggttt
     1141 actggatagt cgtgaagtaa ccattgaagt tgaagaccaa aagaaacctg caccaatgat
     1201 gaacaatgga ttggagcaaa tggggatcga cctaaatgaa actctaggtg ctttgacacc
     1261 gaagaaaaaa gtggaacgga cggtaacagt caaagaagct ttagagttat tgatcaagga
     1321 agaatcagca aaattagtca aggatgctga tatccatagt gaggcaatca aattagcgga
     1381 agaaagtgga attattttta tcgatgaaat cgataagatc acttctaaat cacagcaatc
     1441 tggagaagta tccagagaag gcgtccaaag agacatattg ccaattgtcg aaggttcaca
     1501 agtaaacaca aaatacggga tcatccaaac agatcacgtc ctattcattg cttcaggagc
     1561 tttccatttg tctaaaccaa gtgatctaat tccagaactt caaggacgct tcccgatacg
     1621 ggtagaatta gatgatttga cagctgaaga ttttgtgaag attctgaccg aaccaaataa
     1681 tgctttagta aaacaatacg tcgcgttgat cggtacagaa aatgtgtcag ttacgtttac
     1741 aaaagaagca attgaacgga tcgctcaaat cgctttcgat gtcaaccgtg acacagataa
     1801 tatcggcgca cgcagactgc ataccatttt ggaaaaacta ctagaagatc tattatttga
     1861 agcaccagat atgcaaatgg gagaaataac gatcacagaa gcatatgtgg atgacaaact
     1921 tggctcaatt gcggcaaatg aagatttaag tcgctatatt ttataaaaaa tcggaggacg
     1981 acaatgacag acttgctaac gaaaacccgc aagataaaca cacttttgca gcaaaagaat
     2041 acttttgaca tgcaagcaga tttgccttac gataaaatgg ctgtcacact aggtggtatt
     2101 cttgacagca acacctatat cataggaagt gacggagtat tactaggtta taatgaacgt
     2161 cacgatgtga acaatgaacg aatcaaaaca atgtttgttg agaaacaatt tcctgaatct
     2221 tatactgata cagtggcaca gttgatgaaa actgaagcta atatcccaat tactagtgat
     2281 attactgcat ttccggtaga gtcaagagag aaatatccat ttggtctgac gactgtcgtt
     2341 ccgatctttg gagctggcga acgtttgggt acgattatct tatctcgtat ggaacaatcg
     2401 tttgcagatg acgatctagt cttagcagaa tatggagcta cggttgtcgg tatgcagatc
     2461 ctttatcaga agtctcgaag catcgaagca gatgtacgga gtgcaacggc tgtccaaatg
     2521 gcaatcaaca cattatctta tagcgaattg aaggctgtac aagctatctt tgaagcattg
     2581 gatggagatg aaggtcgtct gactgcttct aatattgccg atagtatcgg catcactcgt
     2641 tctgtgatcg tcaatgcttt acgaaaattg gaatcagctg gaatcattga atctcgttcg
     2701 ttaggcatga agggaactta tttaaaagta ttgaattccc gttttaaaga cgagttagca
     2761 aaacacagtt attagaagta aggggactgg ggcagataga aatgtttgtt ccagccttcc
     2821 cattttaaaa agtaagtcga agttggagga atggcaatga ctgttattat tcaaaatgac
     2881 cagctgatcg ccgaaatcgc tgaacacggt gcggaattga tcagtctgaa atcaaaagaa
     2941 catggtttag agtatatctg gcaaggtgat ccagcgtatt ggggcagaca tgcaccggtt
     3001 ttattccctt ttgttggacg tttaaaagat gatcagtatg tttatcaagg gaaaacatat
     3061 caaatggggc aacacggatt tgctcgggat atggattttg aagtcattga acatggacaa
     3121 gaaatggcta gtttcatctt aaggagtacg ccggagacat tagaaaagta tccatttgat
     3181 tttgaattag tgatcagcta tgaattagga ggagacggca tcacggttca ttatcaagtg
     3241 gcaaataccg gtgaaaaaga aatgttcttt tctatcggtg gacatcctgc tttcaatgta
     3301 cctcttgaag aaggattgtc atttgaagat ttctatctgg cattttctcc attgaaatca
     3361 agattacgcc taccattgaa aggcccattc atcgatatgg atcaaaagac gttaggtcag
     3421 acaaatacga atctggcttt gaatcgtgaa ctttttgagc aagatgcctt ggtttttgaa
     3481 acaaggggtc taaatgcgta cgccattcga agtgaaaaat cttcccatag cgtaacttta
     3541 agctataaag atatgcctta tgtagggatc tggtctcctt atccaaaaga agcgcctttc
     3601 gtttgtatcg aaccgtggtg cggaatcgct gacacggtga acagtagtgg aaacttgatg
     3661 gaaaaagtag ggatcaatca actaggtgcc catgaaatct tcaagacaaa atattccatc
     3721 acagtgaagt aagaggttgt gaccgagaag ttagcaggga acaccgttta ccaaggtgtg
     3781 taaaaaagct atcctgtaag taataaggga gtgtgacatc gatttgtcac actcccgttt
     3841 tatctctttg gaatgaaaaa gtatcaggat tttttcttgc tgctattcaa accaaacggg
     3901 attcgacttt cagtatggtt tctgatacgc ttgatattgt ctttatgccg ataaaagata
     3961 aaggaagaaa ctaaaaaggc aatgatcgtc agtaaccaat tgggcttagc caaaatcaca
     4021 ggagctacat aaggaaggac gatcgtagat aaagtgatca atactgcact gatcatactg
     4081 ctcaaactca ccatacttgt acaaaataaa ctaacgataa agatgataca agaataaaca
     4141 aaaaatacag gattaaatcc taaaagcatt cctgcacttg tcgcaacagc ttttcctcct
     4201 ttgaacccag caaaaatcgg aaatgtatgg ccaagaatgg cgcatacccc aaaccaaaga
     4261 gggttaatcc cagaaaggtg gaagatcatc ggcaaacaag tagccaatgt accttttaaa
     4321 atatccataa gcaggacaac aatcccggct gttttgccta atacacgaaa tgtattggtc
     4381 gttccagtat taccgctccc gtgttgtcgg atatcttttt tgaaaaacaa tttgccaatc
     4441 cagacaccgg aaggaataga acctaaaaga tatgctaaaa taagtaataa gatcaatttc
     4501 attttttacc ctctttcaag ttgaaaacta tcctttattt tagcatgaat aggtaacagg
     4561 aacaattgaa aaaatgaaag atacaagtat aatagatgga ataaaaagaa aagcaagggg
     4621 tggcagaaat gtctgtaaaa aatccaacac aagaaacgat tttcaactat ttggaagaag
     4681 caaaaaatat tgccgtcgta ggcttgagca ataaaccaga acgaacaagt tacatggttg
     4741 cacaggtatt gcaagacaat ggatataaaa tcataccagt aaatccatta ttggctggtg
     4801 aagaaattct aggcgagaaa gtctatgcga atttgacaga aatcgatgat ccaatcgata
     4861 tcgtagatgt atttagacgg agtgaatttt tacctgaaat agcaggtgat tttttgaaaa
     4921 ctgatgctaa agtgttctgg gcacaattag ggattgaaaa caaagaagca gctgagatgc
     4981 taagaaaagc aggacgtaca gatatcgtaa tggatcgttg tattaagatt gaacttggta
     5041 agttgaaaga aaaccaaaaa taaaaatgac cacaaagaag agatgatcga tagaggggaa
     5101 tgtcgaaaaa gcggttagga gataattaaa attgatttct cgtctaccct cttttttttc
     5161 acgacctttt ctagtatgat aatcgtggtg cggttttgcc gcaaagtatt attgaaggag
     5221 cgtttgcctt ggcaaaaaaa gtaaacaacg aatacaatga ctcgtccatc caagtattgg
     5281 aaggattaga ggcagttaga aaaagaccag ggatgtacat tggatccact gacagccgag
     5341 gcttgcatca tttagtctat gaaatcgtcg ataatgcagt tgacgaagca ttatcaggtt
     5401 atggcagtga gattgatgtg accatccatg aagacaatag tataactgtt gcagatagcg
     5461 gacgtgggat gcctgtagga atgcatgctt ccggaatacc aacagttgaa gtgattttta
     5521 ctgtgctgca tgctggcgga aagtttggtc aaggagggta caaaacttct ggtggactgc
     5581 acggtgtagg agccagtgtg gtaaatgcct tatcgaaatg gctaattgta acgatcgtta
     5641 gagatggcgt agagtatcag cagaaattta aaaacggcgg taaacctgac ggtactttga
     5701 aaaagatagg aaaaacaaaa aaagcaaacg ggacgactgt ccatttttta ccagatgata
     5761 caatattcag tacaacgaaa ttttcatatg aaattttagc cgaacggtta agagaatctg
     5821 cctttcttct aaaaggcgtt aaaatctctc tttctgatct tcgtggagaa gaaccagtca
     5881 aggaaatttt tcattacgaa gaaggaatca aagaatttgt tgattatctg aatgaagaaa
     5941 aagatacatt gacaccagtt gtttattttt caggagaaaa agaaggtatc gaagtagagg
     6001 tcgcttatca atacaatgac ggttattcag aaaatgtttt atcttttgtc aataacgttc
     6061 ggacaaaaga tgggggaacc catgaagctg gtatgaaagc ggcgatgacg aaatcttata
     6121 atgaatatgc gagaaaagtt ggtttgctga aagaacggga caagaatctg gaaggtagtg
     6181 atttcagaga agggttagct gctgtacttt ctatccgtgt acctgaaaat cttctgcaat
     6241 ttgaagggca aacaaaagaa aaacttggta cgccagttgc tcgtaccgta gtagataacg
     6301 tcatcagtga acaaatgggc ttttatttgc aggaaaatag cgaaatgagc cagatgttgg
     6361 tccgtaaagc aatcaaagcc agagaagcga gagaagcagc acgtaaagcc agagaagaaa
     6421 gccgaaatgg gaagaagcgt aaaaaaggcg aatctcttct ttcaggaaaa ttgacacccg
     6481 cacaatcccg aaaccctaag aaaaatgaat tgtatttagt cgaaggggat tctgctggcg
     6541 gatctgctaa acaaggaaga gacagaaagt tccaagcaat tctaccttta cgaggaaaag
     6601 tcatcaatac cgaaaaagct aaaatgcaag atatcttgaa aaatgaagaa atcaatacga
     6661 tgatctatac gattggtgca ggagtcggtc ctgagttttc tatcgaagac tgtaattacg
     6721 acaaagtaat catcatgacc gatgcggata cagacggcgc ccatattcaa gtattactgc
     6781 ttactttctt ttatcgttat atgaaaccgc tgattgaagc tgggaaagta tatatcgcgt
     6841 taccgccttt atataaagta tccaaaggac aaggaaagaa gcaagtcatt gaatatgcgt
     6901 ggacagatga cgaattggca gcaatgatta aaaaagtcgg caaaggatat atgctgcagc
     6961 gatataaagg acttggagag atgaatgcgg aacaattgtg ggaaacaacg atggatccga
     7021 cttcccgtac gttgatccgc gtccgtatcg atgatgctgc gcaagctgaa cgtcgagtaa
     7081 ccacactgat gggtgacaaa gtagaacctc gaagaaaatg gatcgaaaat cacgtgcaat
     7141 ttacgctcga agaagatggc agtatcttag ataaaaaaga ggatactgaa atctctcctt
     7201 cagtttctaa tgatttatta gatgaagaac gagcagataa aaatgaaaac aatcagttat
     7261 ttgaagtaga ataggaggtc acctgatgga aaatcgacaa gaagtacaag aattaacttt
     7321 agaagaagtg atgggtgacc gcttcggacg ttattccaaa tatattatcc aagaacgtgc
     7381 actgcctgat atccgtgatg gattgaagcc agtgcaacgc cggatattat ttgccatgaa
     7441 caaagatggc aatacgtatg acaaaggatt ccgtaaatca gcaaaatctg tcggaaatat
     7501 catggggaat tatcatcctc atggtgacat cagtatttat gaagcgatgg tccgcatgag
     7561 ccaagactgg aagttgcggg aagtactgat cgaaatgcac gggaataacg ggagtatgga
     7621 cggtgatcca cctgctgcga tgcgttatac tgaagccaga ttatccaagt taagtggaga
     7681 gatgctagca gatatcgaga aagaaacagt cgatctcgtg tggaattttg atgatacaga
     7741 aaaagaacca acagtccttc cagctcgata cccaaacctt ctcgtcaatg gatcgacagg
     7801 tatatctgct ggatatgcaa cagaaatccc gactcataac ttagctgaag tgatcgatgg
     7861 cacaatctat atgatcgatc atccacaagc gagccttgaa aaattgatgg aatatattcc
     7921 tggacctgac tttccaacag gagggatctt gcaaggaaaa gatgaaatca aaaaagctta
     7981 tgaaacagga cgaggaaaag ttattcttcg ttcgaaaaca aagatagagg caatgaaggg
     8041 taataaacag caaatcgtga tcacagaaat cccatatgaa gtaaataaag cgaccttggt
     8101 gaaaaaaatg gatgagatcc gtttgaataa gaaaatcgat ggaatagctg aagtacgaga
     8161 tgaaagtgat agaacaggtc tgcaaattgt cgtcgaattg aaaaaagacg ccaatgctca
     8221 aggaattttg aattatttgt tcaaaaacac tgagctacag atcaattaca atttcaatat
     8281 ggtcgctatc gaccatatga ctccgcatca agttggactg aaagacattt taagaagtta
     8341 tatcgagcat cgaaagcaag tcataacaaa acgtagtcaa ttcgatttag ccaaagctca
     8401 aaaaagacag catatcgtag aaggattgat gaaagcttta tcgattctgg atgaagtaat
     8461 cgcaacgatt cgtgaaagta aagacaaaaa agacgccaaa aagaatttag tcgacgtctt
     8521 tcaatttaca gaagaacaag cagaagcaat tgtcacattg caattatacc gtttgacgaa
     8581 cacggatatc acagagcttc aaaaggaatc cgaaagcttg attgcgcaaa tcacagagtt
     8641 gaataaaatc ctttcaaatg acaaagagct tttctctgtt atgaaaaaag aattgcgaga
     8701 agtgaaaaaa aattattctt ccgctcgtct aacgacaatc gaagacgaaa tcgaagaaat
     8761 caaaattgat acgcaagtct tagtggcaca agaagacgta attgtcagtg tgacgcgaga
     8821 aggctatgtg aagcgaacaa gccttcgttc ttatagtgct tccaaaccag aagaaatcgg
     8881 gatgcgagaa ggcgactact tgctatactc aggtgaattg agtacattgg accatgttct
     8941 tctgatcaca aataaagcaa atgtgatcta ccgccctgtc catgaactac ccgatttgaa
     9001 atggaaagat gctggtgagc acatttctca aacaatctcc aatctgtctg tagacgaatc
     9061 gattttagct gttttcccat atcaaaaaat tcaagcagag aaaacatttg tattcatcag
     9121 caagaatgga ttgatcaagc aaacacgaat gacagagttc gaaccatggc gcacctataa
     9181 gagccgtccg ctcagctgta tgaagctaaa ggcggaagaa gatgaactga ttgctgtgta
     9241 tctgaaagaa gatcagccag gcttagatgt tttcttagta acgcatcaag gaatgggttt
     9301 gcgctatcct ttaacagatg ttcccgttgt tggagcaaag gcagctggag tgaagtctat
     9361 gaatttgaaa gatggagata ggattatcaa cgggctgctt gtgttagcag aaggtgatac
     9421 gccgatcgta atcgttactc agcgaggagc tgttaaacgc atgcttgctc aagaaatcag
     9481 ccaaacttca cgtgcgaagc ggggagtgac ggtcatgcgt gagctgaaaa aaaatccgca
     9541 ccgtattatc tatatgtcgg aaggaagatc aaaagaaata accttgatga atcaaaaagg
     9601 gcagcagtta acgattgacc caactgattt ccctataggt gatcgaacat cgaatggatc
     9661 atttgcgatg gatgagaaaa aaggtggaga agtcatcgaa gttatcgatg ctcctgagat
     9721 tttcatcgag tcgtaactgt tgtagtattt taatgaagag acgccatctg tattaataca
     9781 ggtggcgtct ctttttcatt catttttaat aaatccttat acgtttgcaa aaaaagatga
     9841 aagtgttcac aatgcttaaa tgttggtatg tcaacgttga tttaaatata tgtaaataaa
     9901 acataaaaat tgttaaataa aaaacaagaa aacccttgca aagatagggt gctgtgttat
     9961 actaatcacg taaaaactgt tacataaaac agaaatttag gaggtccata caatggagca
    10021 atggaatgga tttaaaggta ccaaatggaa agaaggcgta gacactagag actttattca
    10081 aaataactac actgaatatc gcggtgatga cagctttttg gaaggcccaa cagaagcaac
    10141 agaaaaatta tgggatgaat tgcaaaagtt aaatgatatc caattcgagc gtaacggtgt
    10201 ctatgatatg gacacagaaa tcgtatcaac tattacttca cacgatgcag gctatttgga
    10261 taaagatttg gaaaaaatcg tcggtctaca gacagacaaa cctttgaaac aagcattcat
    10321 gccgttcggc ggaatcaata tggctaacaa tgcgctaaaa agcaatggct atacaccaaa
    10381 cgatgaattg acacatattt tcactgactg gcgcaaaaca cataatcaag gtgtatttga
    10441 cgcttatact gctgaaatgc gtgctgctcg taaaaacaaa atcattacag ggcttccaga
    10501 cgcttacggt cgcggacgta tcatcggtga ctatcgtcgt gtggcattgt atggaatcga
    10561 ctatttgatg gaacaaaaga aaaaagatca tgatatgact ggttatcata caatgagtga
    10621 agaagtgatt cgtttacgtg aagaaatcac tgaacaatat cgtgcattag cgcaaatgaa
    10681 agaaatggct gcgaaatatg gctatgatat ttctaaacca gctgcaaatg caaaagaagc
    10741 tgtccaatgg ctatacttcg gatatttagc agcaatcaaa tcacaaaatg gtgctgcgat
    10801 gtcaatcgga cgtatctcag cattccttga tatctatatc caacgtgatt tggataatgg
    10861 agtaatcaca gaaaaagaag cacaagaatt gatcgaccac ttagtaatga aattacgtat
    10921 ggtcaaattc gcgcgtacac ctgaatataa ccaattattc tcaggattcc ctatctgggc
    10981 tactttatcc attgcaggta tgggattaga tggtcgttca ttagttacaa agaatgattt
    11041 ccgtctgctt catacattaa caaatatggg accttcacca gaaccaaact tgactgtttt
    11101 gtattctgaa caccttccag aaggattcag aacatatgct agcagaatcg ctatcgaaag
    11161 ttcttcgatt caattcgaaa atgataatca gcttcgtgaa caatggggtt ctgatgattg
    11221 tgcgattgct tgctgtgtat caggaacagt tgtcggaaaa gacatgcaat tctttggcgc
    11281 tcgtgctaac ttggctaaaa cactattata tgccatcaat ggcggagtcg atgaagtgac
    11341 tggcgcccaa gttggaccag aatatcgtcc aatcacatca gaaatactag actttgaaga
    11401 agtgaaacat aacttcatga atatgatgga ttggttagca gaactatatg tcaatacgtt
    11461 gaacatcatt cattatatgc atgataaata ttcttatgaa gcagctcagc tggctttgat
    11521 ggattctgac ttgcaacgca catttgctac aggtattgct gggatctctc atgcggcaga
    11581 cagcttgtca gcgatcaaat atgcaaaagt aaaagcaatc cgtaatgaaa aaggaatagc
    11641 agttgatttt gaagtggatg gcgacttccc taaatacgga aatgatgatg accgtgcaga
    11701 tgaattagtt tcatggatct tggaatactt catgggacaa atcaaacgtc ataaaactta
    11761 ccgcaactca aaaccaacaa cttcattatt gacgattact tcaaatgttg tttacggtaa
    11821 aaatacagga aatactcctg atggccgtga agctggaaaa ccacttgcgc caggagcaaa
    11881 tccaagttac aatgcggaac aaagtgggtt attagctagc ctgaactcaa cagctaaaat
    11941 gaattacggc tgtgcattgg atggaatttc aaatacccaa acaatcaatc cttcaggtct
    12001 tggaaaagaa caagatacaa gaatcgaaaa cttgcgtaat gtcttagatg gatacttctc
    12061 aaaaggagct tatcatttga atgtcaatgt attcagtaat gatttattgc gtgatgcaat
    12121 ggaaaatcca atgaaatatc cgaacttgac gatccgggta tctggttatg cagttaaatt
    12181 ccgtgacttg acaagagaac aacaattaga tgtcttgatg agaacttcac acgacagaat
    12241 gtaaaaagtt cgtgaaaaaa gtttttcgac ttagcgtcga aaggacgttg aatagccatc
    12301 gcatcaaaat cgaataatca gtatgcttat ttgaaagaaa cgatttacag gggcaggact
    12361 tgtatgttcc gtccctgtaa attttacaca taacgaagcg gcaattgcga aaaattttgt
    12421 tcagaaagct tcgcttcgac agctaacttg tacttaaaat gaaatcaaag aaaagatgtg
    12481 aacttaaatg gaagaaaaaa caatcggcta tgttcattct atcgaaactt tcggttcagt
    12541 agacggacct ggactgcgct ttgtcgtgtt tatgcaagga tgccgcatgc gttgccaatt
    12601 ttgtcacaat ccagatactt ggaatattgg cggagggaaa gaatacacag cagatgaact
    12661 tttagataaa gcagaacgct tcagacctta ctggggagat aaaggtggta ttactgttag
    12721 tggtggagaa cctctattgc agatcgattt tttgatcgaa ctatttaaaa aagcaaaaga
    12781 gcggaagatg catactacat tggatacatg cggaaaacct tttacgtatg aagaaccttt
    12841 cttttcacgt tttcaagaat tgatgaagta tacagattta ctgttatttg acatcaaaca
    12901 catcgataat gaagagcata aaaaactaac gcattgggac aatgaaaata ttttagaaat
    12961 ggctcaatat ttgtcaaaaa tcaacaaacc ggtttggatt cgccatgtac tagttcctga
    13021 gcgaagtgat tatgatgaat atttgatccg actagacaac tttattcaaa ccttgtcaaa
    13081 tgtagacaga gtagagattc ttccttatca tacgatggga aaatacaagt gggaaacact
    13141 tggtttgaaa tatccattag aagggatcga accacctaca aaagaacgtg tcgaaaatgc
    13201 taaacgattg cttcatacag aagagtatac aaaatattta acgcgatagg tcgcatcgga
    13261 aagaagataa gtattgcttc tttccgtttt tttgctatta ttaaatagga gaaatcacaa
    13321 aggagcgaat gacatgtcaa aaattttagt ttttggtcac caaaatccag atacagatgc
    13381 aatcggggca gccattgcgt tttcacactt gcaaaatgaa ttaggtaaag aaacagaagc
    13441 agttgctcta ggtacaccta atgaagaaac acaatacgca ttagaccact ttcagctatc
    13501 tgcaccacga gtagtagagt cagctaaagc agaaaatgca gatgtcatgc ttgtggatca
    13561 taatgaattc caacaaagta tttcagatat cagcgaagta aatattttag ctgtagtaga
    13621 ccaccatcgt attgcaaact ttgaaacagc taatccattg tactatcgtg cagaaccagt
    13681 tggatgtaca agtacgatta ttttaaaact atataaagaa aaccaagtaa caattcctaa
    13741 agaaatcgca ggaatcatgc tctcagctat catttcagat acattgttgt tcaaatcacc
    13801 aacatgtacg cctgaagatg aagcagctgc aaaagaactt gcagcaatcg ctgaagtcga
    13861 tctaaacact tatggacttg aaatgttgaa agcaggaaca aatctaagtg ataaatcagc
    13921 tgcggttctt cttgatttgg atgcaaaaag tttcccaatg ggcagttcca acgttcgtat
    13981 cgctcaagtc aatacagtgg accttaatga agtgatggat cgacaagcag agctagaagc
    14041 agcaatggaa gatgaaatga aagcaaatgg ctacgatctg ttcgtattag ttgtcacaaa
    14101 catcttagac agtgattcag aattacttgt tgctggtaaa ggaaaagaaa aagtagaaga
    14161 agctttccaa acaactttga ccaacaaccg tgctttgctt aaaggtgtcg tttcacgtaa
    14221 aaaacaagtc gtacctcaat tgacggctgt atttagctaa tcaaatggta gtatgaagca
    14281 ttcaaaatga atggataagc gattgatata aaaagaaaca tagaacatac taaaggccga
    14341 agaaatggag gagaataaca gtcaattatg actcttgttc ttcctccttt ttacgtaaaa
    14401 gccttttaag taaaataaaa cggatagaaa agagaacata tatgaactat tattcaaaaa
    14461 ataaggaaaa cttttatcaa aaactgaccg atgatccgct tttttcttca ttgaccgatt
    14521 atctatatga acatcgagaa caagaaacga ttctaagaga attgaaaaaa gaattttcgc
    14581 aaaataaatt ttcccatttt cttgatttat tgatcgatgc aggattaatc aagagggaag
    14641 aacgaagata tcatttgaac tttccaatat ttgatcctaa agattacttg caacaagcca
    14701 cttcggctgc tgaaaccata gctgaacaat tgaagagact ctctgttgat gaacaaaaat
    14761 tagcgatggg agaagttatt tgggcgtatt gtttcgaaga tgaacggaaa gaagcgtatt
    14821 tttatggagt aagaaacagc cgagagacag aattgctgag gacaactgca ggaaatgaga
    14881 agtaccgttt tatcacactt tcctctatag aacacttccc gcttactttg gctaattact
    14941 tttttgtaca gaagaatcag cttcctgtaa cgaaggcatt caaagaatta gccgaactta
    15001 ttggagacgt aaatgaatcg tacttttttg atcaaataga agttatcgtt gacaggatac
    15061 gtaagaataa atataaaaat cgccggccta gtattttcca tcagtctcta cttgtgacga
    15121 atactataaa ggaagaagaa tcattcacat tagaattacc tattgtcgaa aaaaacaatt
    15181 ttgagattga attaccaaca ttggatcctt cattgacaat ggaggaaatg gcctttctta
    15241 aacgacaaat cttttctgag ctgagcaaga aatttatacc gcatgcattt tcttatatca
    15301 aagaatatgg aacagtttta gtgagtaaaa cctaagagag cagtaagcaa aaattatact
    15361 gactcttgca ggaggagcct ttctcttcct ttatggttaa aagaattatt tgagtatttt
    15421 gcctatctac cacctattag attcactaca tggtctggaa acaaaggaat gcaaaaaaat
    15481 ggtgagaaat gtggaatgac aaaggaagta gtgataagaa aagttagatt tttaaataat
    15541 caatattatg attcagtcaa gtatggcatt ttatgggaag aactagctga ttagattagg
    15601 aagtccattt gataatccag aatcactaag aaacgaatgg cgagtcatta ataatttatc
    15661 caatcttggg ttattctttt agattgaagt gtttaaaact aaaaaagcat ttatgaagtc
    15721 gtaatagtca ggcttcaaaa tgctttttat tttgtttgtt ttttctttgc cacataaata
    15781 cacctagact gacgatgaag agcatatcca acagatttcc tgcgacttgg atcgtcgaag
    15841 ggttggtcag tggcattaaa gcaaggacaa aaaaggaaaa caagctgtaa ataaaacagg
    15901 tgacaggaac taatcgaaag aagagcggac atttcaattg gctaaaaatg agtgaaaaaa
    15961 taaaataagt aaaaatggat aaaatcacga ctaaaccatt catgataaag aagaaaagcg
    16021 tattgtgatt aaacgaataa aaaagaattg ctaaaaaaga aaacgtttta aatgcaatca
    16081 atacgcttga agcttcgttt aacgtaccag gatgtcgatg aaaaaaatag gcaccgaaat
    16141 aaacagaaaa cagtaagccg caaagagaag acaaaatcaa aaaaaacgga ttttcttcgg
    16201 cttctgtgat atcagaatga tccaaaagca accagcctaa tgcactagaa ggcgcatgtg
    16261 aggtcttaag gtatgaataa catagaaaaa ataagatcgt caaagttgca aaaaaagagc
    16321 ctaaatgtaa aaaattagtg gttctacgtt gaatttccat aatatctcca aattaaattg
    16381 ataagtatat aaagtataat aacaaattga tgagtatatg tcgatgtttt ttaatcaaaa
    16441 taataaataa aaactgtcta acatttaaaa tttaatttag atattagaca gtttttaagt
    16501 ttgtgagtta ttttccttgt cgttttctca cttttttagt ccagaaacca cctgaaatat
    16561 attttggctc ataagaaatg ataaaagcac gctcttcgat ttctttgatc aggcgataca
    16621 gttcacgttc gctttttcta ggagaaagta tttcgagaac tagccgctca ccttccagcc
    16681 ccattgcaga agtctgtgtc acaccataac cgtgttctct cagtataccc ggcaaacttt
    16741 tatcttctgc gctatttgct ggcaaaatcg ttgtgaccat gatgtatcct aatgccaatt
    16801 tgtcttcaat caaaattccg acactgatac ctactgcgta acctaacgca tagacaaata
    16861 aattcagtgg attatctaaa cgatctaaaa caagggataa gccgacgata taaatcgtga
    16921 tctcgaccat actgacaagt ggagcaatca gtcgatagcc tttcattgtc aacatgaaac
    16981 gtaacgtgtt cagagtgata taagcaaaat taatgaggaa gatcatcccc aacatttttg
    17041 gatcaatcat ttgtaaaact cctttcattt atataacaac aatatcaaaa aaaatcgtaa
    17101 gtgaagaaaa attgaaactt tatacataca aaaaaatgat aaaatgatta taatagaatt
    17161 ataaagatag gttaggtgat taaaatgaaa acggtcaatc gtgtgttttg tgccagccac
    17221 gtgctaagtc tccttctcag tatatactta ggtgtctatt cgattcaaca gccaattgag
    17281 gtagagaaca ttgtttttca gatgtcgctg acagatggtt taattttaag cgtttttttc
    17341 tttttgatac tgtttattgg taatattttc ggtagcgttg ccagtttttt gaatttttct
    17401 atttatccac tcctttcgct agcattagga gtggtgggac tttgttctct gtgcttattt
    17461 cccgagccat tcagtcctgc atttatatta tttggtacac tgaacctgtt ccagacaact
    17521 gttggagcat ggttgctatg gcgatcagga aatttaatga aaataggtga gtaacaattg
    17581 aatccaatta tattgaaacg ttctgtccat catattccag tgttagaagt tgttctgaaa
    17641 gaattaagag aggaacgtat tcccgttgtc atctattatc acggatggca gacaagtaag
    17701 gagttagttt tgacgcaagg aagaaaaatt gcgcagcagg gcattcgtgt gatcctgcca
    17761 gatgcgatga atcatgggga gcgcaaacag ccggtttctt ctattccatc ctatacattt
    17821 tgggatagta tctacggaaa tctgtttgaa tttgacacac ttatcaagca tttagaaaaa
    17881 agaggtcttc tacatgatca gcttgcagtt ggcggggttt cgatgggggg aatgacaaca
    17941 tgcgccttgt tggctaaaca tcctcatata gtagcgggcg tgtgtttgat gggttcccct
    18001 gcacctttgg aatatggaaa agaaatcgta aggcgcgcat cggaatataa ttatgtcgta
    18061 ccaaaagact attttgattt gatttcatgg gttaagagct atgatttgtc attgcagccg
    18121 gaaaagttag ctggacgtcc tctgttcttt tggcatggac aagctgacga aaaaatccct
    18181 tatatacaaa cggaacgatt tgtagaaaaa aatcggcata agtcttacgg aaaagaaatt
    18241 gtttttcgtt cctcaaaaac acagcgacat atggtacaaa taccactaat ggaagaagct
    18301 tccacttttt tagcaaaaaa tttgataaaa aagtataaat aattgaaacg gaaaatcact
    18361 catgacgata gaggattttc cgttttttat tttgggctat aataaactct gttaggaaaa
    18421 tttggtataa ttgatagtaa gaatgacaac atgttagaaa ggaagagagc atgcccactt
    18481 tagcagatgt tgcaaaacgt gccaatgttt caaaaatgac ggtctctcga gtcatcaatc
    18541 atccggaaca ggtgactgac gaattgaaag agttagtttt ctcagcaatg gctgaactgg
    18601 attaccgtcc aaatattgct gcaaaagcac ttgtcacgaa tcggtcgcag atcatcaaat
    18661 tatttatact ggaagaaatc gacacaactg agccctatta catgaatttg ctgatgggaa
    18721 tcgctaaacg gatcggtaaa gcgcattatt ctctccaatt agttacaaat gatgccttcg
    18781 acgtcggttc ttgtgatgga tacatcatca caggtgtcag aaaaaaagac ttcgattgga
    18841 tcgaccgttt ggaaaaacca gttgtcttgt ttggggaaaa ttctgaagga tatgattatg
    18901 tagacagcga taatgaatat ggtacagcaa aagcaacgca atatgcctta gaaagagatt
    18961 atcaaacgat tatttatatc ggtatcgaag tggacgagcc ttttgaaatt tcaagggaaa
    19021 ctggttattt atctgtgatg aaggaagttg atcttcctag taaaatcttg cggtttccaa
    19081 atcattcgac agaagtagaa agatatatga cagaaaactg ggcagatttt acagataaaa
    19141 cttgttttgt atgtagttca gatcggctag cactaggagc agtaagaggg attataaatg
    19201 ctggaggaca cttgcccgaa gaatttggtg tgattggatt tgatggggta tttttagacc
    19261 aaatcagttc acctaaattg acaacgatca agcaagatat tatacgtatg ggtgaagtat
    19321 gtggagaaat gttgttaaaa aaaatagaag aaaaaggggc gagtcaaggt tatcgtcgtt
    19381 tttttcctga actagttatc agagagacaa cgaattaagt cttattacta tatatatatt
    19441 tatgaaattc atcgctattt tcttaaaata atcagcgcat tgctactgaa aacaatgaat
    19501 ctgtgctatt attgagaaaa gttccatttt tgagaaggat ggtttgaatg aaagaaatca
    19561 gacgaaaaca tttccgtttt cctgcgtacg atgatgagtt aggcgtaaaa ttaaccaacg
    19621 acaagaagcg agtattattt gacggacaag aagatctttt gactgaacag aatcagtccc
    19681 cgtttgaaca gatgaaagaa tttcatttca cgaacacgaa caccgagaaa atccgtcgca
    19741 agcgtcgtgc aacaaatgga ttgaagtcag ctccgcgtaa ggaaaatttg gaaaagcata
    19801 aaggaaaatt accggattat accggccatt accaatcaga aatcacatcg acaggacgaa
    19861 aaaaactttt tggtgattcc tctcgttctg tcagacaaag tgaaacaagg acagagtcca
    19921 agcatactac acgaaacgca tctgttgaca cgaaggtgcg ggagcgttct tattttgttc
    19981 ctaaatatgt acccgcatcg attatcccag accctagaga aagcaaaatt tctgaggatc
    20041 atttaatcga atcgatgaaa aaaacggatg actcttatct gttgttcgat acagaaccga
    20101 cgccgtatca agaaaaaaga aatggtgagc cgtctgtacg aacattcaat cgaacaaaaa
    20161 cgttagagga aacaaagcag tcagaagaaa aaaaatcgaa aaaaagccat ggtatcttag
    20221 aacgctcatt acaaggattg atcgatgatc aaggaaactc tttgggagaa aatagctatt
    20281 ttaaataagt gatatacaaa tttatattcg gctacagttg gaaaggaaac gctgcggcgt
    20341 ttccttattt tatactctat ccgaaagttt atatcgtatc ctcaaatttt tttgagttta
    20401 agcgaatttt ttctcccaat aagtaagaaa ataatggcaa aataaaaaaa caaactattt
    20461 tcgcagatcg tttgcaaaaa tagtt
//
LOCUS       DARBET010000046        19993 bp    DNA     linear   BCT 07-NOV-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4027
            SAMEA114569841-rid20112183.denovo.046, whole genome shotgun
            sequence.
ACCESSION   DARBET010000046 DARBET010000000
VERSION     DARBET010000046.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA114569841
            Sequence Read Archive: ERR12203666
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 19993)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 19993)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001972618.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001972618.1
            Genome Coverage       :: 132x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/07/2023 13:18:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,041
            CDSs (total)                      :: 2,975
            Genes (coding)                    :: 2,908
            CDSs (with protein)               :: 2,908
            Genes (RNA)                       :: 66
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 67
            CDSs (without protein)            :: 67
            Pseudo Genes (ambiguous residues) :: 0 of 67
            Pseudo Genes (frameshifted)       :: 26 of 67
            Pseudo Genes (incomplete)         :: 28 of 67
            Pseudo Genes (internal stop)      :: 20 of 67
            Pseudo Genes (multiple problems)  :: 6 of 67
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19993
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA114569841-rid20112183.denovo.046"
                     /isolate="VRE4027"
                     /db_xref="taxon:1352"
                     /country="Denmark"
     gene            complement(57..590)
                     /locus_tag="SCJ86_001829"
     CDS             complement(57..590)
                     /locus_tag="SCJ86_001829"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336938.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF402 domain-containing protein"
                     /protein_id="HEG1169367.1"
                     /translation="MGVPKEGEFVTIQSYKHDGKLHRTWRDTMVLKTSEHSMIGVNDH
                     TLVTESDGRRWVTREPAIVYFHKKYWFNVIAMIREKGVSYYCNLASPFLLDDEALKYI
                     DYDLDIKVFPDGEKRLLDVDEYEMHSKMMNYPNDIDFILKENVKILVDWINNGDGPFS
                     EGYIDIWYNRYKQLSRK"
     gene            complement(684..1862)
                     /gene="mutY"
                     /locus_tag="SCJ86_001830"
     CDS             complement(684..1862)
                     /gene="mutY"
                     /locus_tag="SCJ86_001830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="A/G-specific adenine glycosylase"
                     /protein_id="HEG1169368.1"
                     /translation="MNKEWEKWTDKETKEFQDQFIQWYEQEKRNLPWRYNRDPYRIWI
                     SEIMLQQTRVDTVIDYFYRFMEWFPTIEELANAPEEKLLKAWEGLGYYSRARNIQAAA
                     KQIMSEFDGEMPQTPEEISSLKGIGPYTTGAIASIAFGLPEPAVDGNVMRVVSRLFCI
                     EADIAKASSRKIFDEAMRKIIDEKHPGEFNQAMMDLGSAICTPTSPKCETCPIQAFCL
                     ANKRGIQTSFPVKTKKAKPKDVYYISAALQNHSGAYYFEERDSQKLLANMWTFPMMEV
                     TQEEYERLKKEWETKPEIDLFDDLVAEEEQNLPFEKQELFVWQTRHLGEVTHIFSHLK
                     WHVLLFYGRATEGAEQEFTENKTSKWLKPDAFDSVVFPKVQMKLVDQLEKNRNNRNPF
                     "
     gene            complement(1855..2652)
                     /gene="recX"
                     /locus_tag="SCJ86_001831"
     CDS             complement(1855..2652)
                     /gene="recX"
                     /locus_tag="SCJ86_001831"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombination regulator RecX"
                     /protein_id="HEG1169369.1"
                     /translation="MLTVTRISKGKGPFYRVDLSEGEALQVSEDVLVRYRLLKGRELD
                     EETIQEIKKSSGADFGFHLAMNYLSYQLRSEKEIRTYLKEKEILQEDRNKIVARLKEL
                     DLVDDLVYGESYVRTNMRLSDKGPKKLAQQMQQKGLKPEIIEQALSQYTFEDQVQNAH
                     QTAEKAFGKNHGKSQKELLRKIQQTLMTKGFSQEVIQQAIADLPQEADQEIEYDYLVK
                     QGDKLWRRNLRFEPKKRNLKVKQSLYQKGFDLDMIQRFITEKEEESE"
     gene            2759..4141
                     /gene="rlmD"
                     /locus_tag="SCJ86_001832"
     CDS             2759..4141
                     /gene="rlmD"
                     /locus_tag="SCJ86_001832"
                     /EC_number="2.1.1.190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (uracil(1939)-C(5))-methyltransferase
                     RlmD"
                     /protein_id="HEG1169370.1"
                     /translation="MAETIVKTGQTVPLKIKRLGINGEGIGYFKRLIIFVPYALPKEE
                     VLVKITKATPRYAEGELIKVKKTSKDRVVAPCPVYYECGGCQLQHLAYQAQLDFKKDL
                     LLQALEKFKPAGFRSYQLLPTIGMDEPWHYRNKAQFQLRKNKQTQKIEAGLYEANSHQ
                     LVPITDCLVQEPRTQKVMNTVVQLLNKFDAPIYDERTNSGIFRTIMVRIGVKTGELQV
                     VFITRSKKFPQKNAMIREINERLPEVVSIMQNVQAQKTSLVMGDETIHLWGKEAIEEQ
                     INEVTFDLSPRAFFQLNPQQTELLYGEGIKALDVQPNETIVDAYCGVGTIGLSVAKQA
                     KEVRGMDTVPQAIADAKKNAERLGYHNTRYEVGTAEQVLPKWLNEGFQPDGIIVDPPR
                     TGLDNALIQSLLKYPPKKLVYISCNVSTLARDLVNLSKKFKIEYLQSVDMFPQTARCE
                     VVVKMTRLAD"
     gene            complement(4315..4893)
                     /locus_tag="SCJ86_001833"
     CDS             complement(4315..4893)
                     /locus_tag="SCJ86_001833"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310335.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lytic polysaccharide monooxygenase"
                     /protein_id="HEG1169371.1"
                     /translation="MKKISTLLFIGLVSLGLFGFSIPGFAHGYITSPGSRAYLGTSAA
                     GNLNQNVGRAQWEPQSIEATKNTFIDGKIASAGVSGFEPLDEQTSNRWHKNMVNPGTL
                     NITWNLTAQHRTSTWDYYITKPTWNPNQPLKFSDFELITKIDDKATVPPKTVNQTITL
                     PQDRKGYNVILAVWNISDTTNAFYQVIDVNIQ"
     gene            5215..5685
                     /locus_tag="SCJ86_001834"
     CDS             5215..5685
                     /locus_tag="SCJ86_001834"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629457.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan-rich sensory protein"
                     /protein_id="HEG1169372.1"
                     /translation="MKMIKDIRFWVCVIGIVILGFLSGLLSGNPGKYYYSLQLPPFAP
                     PSWIFGPMWTLLYILMGISLYLLLNHNNKKQRNNLVGLFVIQFIFNFIWSALFFNLRN
                     IFIAAIDITLLVIFLSVLMYQLWLHHRLTMWLMIPYYLWVLFATLLNYSIYFLN"
     gene            complement(5793..7019)
                     /locus_tag="SCJ86_001835"
     CDS             complement(5793..7019)
                     /locus_tag="SCJ86_001835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322158.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LCP family protein"
                     /protein_id="HEG1169373.1"
                     /translation="MKRWQKVLLTLLGVFVLLIAGLSAYGIRFMGEASQTVNKISKNS
                     NRISSKRSERVSIDDKEPFSVLLLGLDTGGLGRTEQGRSDTMMVVTVNPKQKKSTIIS
                     LDRDIYTNIVGYGTVDKLNHAYAFGGVEMAMDSIEQLLDIPIDHYVTINLDGMEDLIN
                     AVGGIKVNNKIDFTLDGVHVPVGEQTLNGEKGLAYSRMRHEDPEGDIGRQRRQREVVT
                     KIVNKVLSLDGVSNYRKILKAVENNVTTDLDWDDMLDVATNYTPAFETIKQDQLQGEG
                     QMINDIYYQILGKNDLLSIQNELKKQLNIKTSDTLPNLKNENASMMFFDDSEDGDSTN
                     GDGSNSEGYTDYSSYSENTYDQYQDPNTYYQQTEDTSQYYTPSQDTQSYDPYAGQEQN
                     GYGYDQNNEYGTTSGY"
     gene            complement(7773..7973)
                     /locus_tag="SCJ86_001836"
     CDS             complement(7773..7973)
                     /locus_tag="SCJ86_001836"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003230776.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cold-shock protein"
                     /protein_id="HEG1169374.1"
                     /translation="MEHGTVKWFNNEKGFGFITVDGGDDVFVHFSAIQGDGFKSLEEG
                     QEVEFTIVEGARGPQAAEVTKL"
     gene            complement(8107..8715)
                     /locus_tag="SCJ86_001837"
     CDS             complement(8107..8715)
                     /locus_tag="SCJ86_001837"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298541.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="superoxide dismutase"
                     /protein_id="HEG1169375.1"
                     /translation="MTYTLPDLPYAYDALEPYIDEETMHLHHDKHHNTYVTNLNSAIE
                     KYPELGEKTIEELLSDMDAIPTDIKTAVRNNGGGHANHSFFWEIMAPNAGGEPTGEIK
                     EAINEAFGDFSSFKEEFKKAAAGRFGSGWAWLVMENGKLAITSTANQDSPLMEGKTPI
                     LGLDVWEHAYYLKYKNVRPDYIAAFWNVINWDEVNKRFEAAK"
     gene            complement(8800..9270)
                     /locus_tag="SCJ86_001838"
     CDS             complement(8800..9270)
                     /locus_tag="SCJ86_001838"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GtrA family protein"
                     /protein_id="HEG1169376.1"
                     /translation="MKQYRQLKGYLEAKGYWEILVYLFFGGLATIVNFVSFALARQYF
                     DLSMALSNSISWFCSVLFAFVTNKLWVFHSKSPNFTHALIECGKFFFYRILSYGLDMG
                     AMVLLINVMNSNEYVAKIITQIIVILANYIFSKLFIFKETEVFEEEVGQSEKDD"
     gene            complement(9318..10232)
                     /locus_tag="SCJ86_001839"
     CDS             complement(9318..10232)
                     /locus_tag="SCJ86_001839"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629458.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation transporter"
                     /protein_id="HEG1169377.1"
                     /translation="MEEGIVPTQPQRSSKKLFGVVVLNAVITLSEFIGGIMSGSLSLV
                     SDAFHNLSDTLAIIFSYGAQKMARKEANKKRTYGYQRLEILSAFINSFILIILSLFLT
                     VEAFKRFTSPEKINSHLMLTVAVIGLLANLFSVLLLRQEADESLNIKSSYLHLLSDTL
                     SSVSVIIGAILIRFFDIYWIDPVITLIISIYILIEAIMVIKKAAAILIQSAPAIDYEK
                     MEQEIEAIDGVKDVHHVHIWQYSEKIIIFDGHIDFEDQLLSEIEKIYPRIISLLKLKY
                     GITHVTIQAETKAKDQKKLIFLNKDESY"
     gene            10378..11181
                     /locus_tag="SCJ86_001840"
     CDS             10378..11181
                     /locus_tag="SCJ86_001840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323479.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1189 domain-containing protein"
                     /protein_id="HEG1169378.1"
                     /translation="MTTKQLIISSFIRFKDLKEARNVPFWKAIVYLLVLSIIMALPIS
                     FQVFQVLNNIQLDGQKIATRIPDFTIQNGQLQTEEKEGFIYQTNSIIFTFDPEGKRTE
                     QDISTDLMGNFVSVGMLKNKLIIALPNTGTTSALLNNNQLELPYTNESLKNLTGKQLR
                     SFLSEASIPLWIKVLTFIFSIYPSFLNLLITLLFTNIAAFLYARFRLTKATFLDCLKT
                     LIYSISLPTIIATILMVFLPSFDTSAFIAFAGIFIFAQAVKGWAKISIN"
     gene            11359..12306
                     /locus_tag="SCJ86_001841"
     CDS             11359..12306
                     /locus_tag="SCJ86_001841"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002368530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglycerate dehydrogenase"
                     /protein_id="HEG1169379.1"
                     /translation="MSERIIYADRPFRDEFIEEIHTIAPGYIFKTELQPEDLQNVEIS
                     LGWNSDYQEKLLASSTLKWVQSISAGVDQLPLETFSKKHILLSNGSGIHSESITEHVL
                     GIILGYSRGLFQAQKAQSDMKWLGSSVHYQSIQGKKILIIGTGHIGKMIAKKCKALDM
                     NCYGINTSGHPAEGMEKTFSLNQLKEVLPEVDFVVNILPLTEETTGLFDQHMFEHFDP
                     KSVFINVGRGASVKTQDLVQALNNQQLSFAALDVFEEEPLPQDHPLWKMDNVLITSHI
                     AGLTPDFQKKLMAIFLTNLKSYFATHELQTNQVKLSAGY"
     gene            complement(12374..12667)
                     /locus_tag="SCJ86_001842"
     CDS             complement(12374..12667)
                     /locus_tag="SCJ86_001842"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303172.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-sulfur cluster biosynthesis protein"
                     /protein_id="HEG1169380.1"
                     /translation="MKLTVTPKAQKWFDSEIDLPETYGIRFFGKVYGKTEVHDGFSIG
                     MSVEQPEHPVKKVEMDGTLFFIEEADEWFFKGYDLLVDYDPNLEEPTYHFKKQ"
     gene            complement(12695..13360)
                     /locus_tag="SCJ86_001843"
     CDS             complement(12695..13360)
                     /locus_tag="SCJ86_001843"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016624078.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TrkA family potassium uptake protein"
                     /protein_id="HEG1169381.1"
                     /translation="MKQNFAIVGLGRFGGSICRTLIEAGQEVLAIDSNEDRVNEYMNI
                     ATHAVVANAQDEMTLRSLGIRNFDHVIVAIGEDIQASILVTLMVKEMGVPNILAKAQN
                     EYHARVLEKIGADRVVHPERDMGIRIAHNLVSKNILDYLELSDKYSLAEIRVSNPKFF
                     NKTLAELNFRQRFDLTVVAIRRVDQTVIASPAADEYVRENDNLLVIGETGDVDILDDK
                     MNQ"
     gene            complement(13500..14132)
                     /locus_tag="SCJ86_001844"
     CDS             complement(13500..14132)
                     /locus_tag="SCJ86_001844"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016624081.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HEG1169382.1"
                     /translation="MIKVLLVDDHEMVRLGVSSYLSIQEDIEVIGEAENGRQGYEKAM
                     TLRPDVILMDLVMEEMDGIESTKAILKDWPEAKIIIVTSFIDDEKVYPAIEAGAAGYL
                     LKTSTAHEIADAIRATQRGERVLEPEVTTKMMEKMSRRNEPVLHEELTNRENEILMLI
                     SEGKSNQEIADELFITLKTVKTHVSNILAKLEVEDRTQAAIYAFKHGLVK"
     gene            complement(14139..15206)
                     /locus_tag="SCJ86_001845"
     CDS             complement(14139..15206)
                     /locus_tag="SCJ86_001845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289154.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HEG1169383.1"
                     /translation="MMGKISRAMLAVYSGIAAFLIILFSLFTYFYASNQSHWWGELLR
                     ARLLYVPLIFHLLAISLGVGLIVFLLLSLIQKTKYGKIEEKLRALSSGNYESKLLLLP
                     IPSASDDLYIKDIDKEITKIKEKMIEVSSELQIVTSRPQYVDGQTKEEILELERHRLA
                     RELHDSVSQQLFAAMMMMSALTEQAEKSETPEMFRKQLKMVAEIINASQSEMRALLLH
                     LRPVNLEEKSLKQGIEQLLKELQNKIQISLKWDVEDVKLSSSIEDHLFRIVQELLSNT
                     LRHAKANELEVYLHKIDNNLLLRIIDDGTGFNMNETKTGSYGLNNIKERVAGIGGTVK
                     IISFKGQGTSVEIKVPLMKEA"
     gene            complement(15203..15934)
                     /locus_tag="SCJ86_001846"
     CDS             complement(15203..15934)
                     /locus_tag="SCJ86_001846"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell wall-active antibiotics response protein"
                     /protein_id="HEG1169384.1"
                     /translation="MNSSWRFFVVVEALLLLFAVWQIVNNTGLLLLVIFGIFNIYLAM
                     RKHPRTKFQNFQLILGSLVIFFSLVNSPALWMMAVFAVLFIGLKGVEISGIDLTKNAF
                     WRKKQIMMIQTEQLKTHNNERKRQQLFGNQRIGNDVYEWDDINIAVISGDTIIDLGNT
                     LLPKDDNIVIVRKGIGRTRILVPLGVAIRLEHATLVGNVLFEEEQFSLKNEQIKIYSS
                     DYDENPRRLKIITNTLIGDVEVIRI"
     gene            complement(16103..16582)
                     /gene="greA"
                     /locus_tag="SCJ86_001847"
     CDS             complement(16103..16582)
                     /gene="greA"
                     /locus_tag="SCJ86_001847"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016172390.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription elongation factor GreA"
                     /protein_id="HEG1169385.1"
                     /translation="MVEKVYPMTLEGKEKLEKELEELKTVKRGEIIERIKIARGFGDL
                     SENSEYESAKDEQAFVEGRITTIENMIRFAQIIDNDGVDADEVSIGKTVTFIELPDGE
                     EEEYTIVGSAEADPFSGKISNDSPIAQALIGKRKNDEVTIATPGGDMLVKIVKVEQA"
     gene            complement(16718..17893)
                     /gene="mltG"
                     /locus_tag="SCJ86_001848"
     CDS             complement(16718..17893)
                     /gene="mltG"
                     /locus_tag="SCJ86_001848"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329049.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endolytic transglycosylase MltG"
                     /protein_id="HEG1169386.1"
                     /translation="MSGNKQHPSKDKKEASKAATADIIDKRRNLRKKEDKIVRKIILV
                     IALTLLIIGGFLGFTVYRYVDSGLKPLDKSDDQLVQVEIPSGSSNKQIGEILEKDNII
                     KSGIVFNYYTKFKNLTGFQAGYYQLAPNMTLDEIGKQLQEGGTSEPTKVADGKIAIPE
                     GYDIDQIAERVAKVTGKDKKEFLDLVNDETFFNELHQKFPELLESASKAENTKYRLEG
                     YLFPATYDYYTSTSLKDLVIEMVNKTNTVMQNYYSAIKQKNLTVQEVLTLASLVEKEG
                     VKENDRKNIAQVFFNRIKANMPLQSDISVLYALGEHKELVTYEDTAVDSPYNLYTNTG
                     YGPGPFDNPSEEAIKAVLEPAENDYYYFVADTSTGNVYFAKTYEEHMELVEKYVNNS"
     gene            18097..19266
                     /locus_tag="SCJ86_001849"
     CDS             18097..19266
                     /locus_tag="SCJ86_001849"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289161.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-lactamase family protein"
                     /protein_id="HEG1169387.1"
                     /translation="MKKGKHAKKRKYSFLMIVVVLFSLSAFSTGTILGWQFYNNEHSQ
                     IVAHAQSAQKKLILTTNEQQLVEKIDQLLRKNDYIGSIYVKKNNRIIMEKGYGYANKL
                     TQQPNDPSLYYQIGSVQKALTTLLILKQVERGHLSLDTKLAQFYPQIAGSQQITIRDL
                     LYMRSGLHRTASPTIPMTDEEVVQFAIQHLKFTDYQTSHYDSLNFTILTGILIKLTNQ
                     PYEKLLTDDIIEPLGLQHTKFYDQVKNSSNHALSYQMSKEDDYFKPLTESETEIHNEL
                     GTGNISMSVYDLNTLFTQVLSGKLVSKDLLFSLWRENANGHPYSGGVYSGDNYILSQG
                     NIDSFDTSAIFSKDSQDAIIMESNIKADKTIKLPTADLRNQIYDLMEGTTKLANS"
     gene            complement(19533..>19993)
                     /locus_tag="SCJ86_001850"
     CDS             complement(19533..>19993)
                     /locus_tag="SCJ86_001850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349576.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEG1169388.1"
                     /translation="VKEMNFQGTYSNEQMENKLEEFENFDYVPIGYKKDQLLLKNHQV
                     TEDGQVIYSSFSPNKDEITFTFKSSKGKTIVLPVFRYKGLTVTVNSKNATVLKQGGPF
                     VTVKSESGINKVTIRYSYTFFSKISLFISIITLIVLLFYCIKLKIRTSHE"
BASE COUNT         6036 a         3995 c         3179 g         6783 t
ORIGIN      
        1 aaaaacagca agcttttgag aaaaatctct cactggctta ctgttctttt ttgtgtttac
       61 ttacgcgaca attgcttgta acggttatac caaatatcaa tatagccttc cgagaacggg
      121 ccatctccat tattgatcca gtcaaccaga atcttcacat tttcttttag gatgaagtca
      181 atatcgttcg ggtaattcat cattttgctg tgcatctcat attcatcgac atccaatagt
      241 cgtttctcgc cgtcaggaaa taccttgata tccaagtcat agtcgatata tttcaaggcc
      301 tcatcgtcca gcaaaaaagg tgaagctaga ttacaataat aggaaacccc cttttctctg
      361 atcattgcta ttacattgaa ccagtatttt ttatgaaaat aaacaatcgc tggttcgcgc
      421 gttacccatc ggcgtccatc agattcagtg actaatgtgt gatcattcac gccgatcata
      481 gagtgttcgc ttgtttttaa taccatggta tctcgccacg ttcgatgcaa tttgccgtcg
      541 tgcttgtaac tttggatcgt cacaaactct ccttcttttg gaactcccat agcaaccctt
      601 ccttaagcag cattttgtgc tttgtgtttg ggtgttcatt acaccgacat cataattatt
      661 atatcaataa aaatgagctt tgcctagaaa ggattcctat tgtttcgatt tttctctaac
      721 tgatctacca gtttcatttg gaccttagga aaaacgaccg aatcaaatgc atcaggcttc
      781 aaccacttcg aagttttgtt ctcggtaaat tcttgttctg ctccctcggt tgcccgtccg
      841 taaaaaagga gaacatgcca tttcagatga ctaaaaatat gagtaacttc tcctaaatgt
      901 cgtgtctgcc aaacaaagag ttcctgcttt tcaaatggga gattttgctc ttcttctgct
      961 actaggtcat caaacagatc gatttctggc tttgtttccc attctttttt caatcgctca
     1021 tactcctctt gtgtcacttc catcattgga aatgtccaca tattcgccag caatttttgg
     1081 ctatctcttt cttcaaaata atacgctcca gaatgatttt gaagagctgc actgatataa
     1141 taaacgtctt ttggctttgc tttttttgtt tttacaggaa aactcgtttg gattcctcgt
     1201 ttgttagcta aacaaaaggc ttggatcgga caagtctcac actttggaga agttggtgta
     1261 cagatagctg aacctagatc catcattgct tggttgaatt cacctggatg cttttcatca
     1321 atgattttcc gcatcgcttc atcaaagatt ttcctgctag aagcttttgc gatgtctgct
     1381 tcaatgcaaa acaatctgct gactactcgc atcacgttgc cgtcaactgc aggttctgga
     1441 agtccaaatg cgatacttgc aatcgcgcct gtcgtatatg gtcctattcc cttcaatgag
     1501 ctaatttctt caggcgtttg cggcatttcc ccatcaaatt ctgacatgat ctgtttagca
     1561 gcagcttgga tgttccgggc tcttgagtaa taaccaaggc cctcccatgc ttttaaaagc
     1621 ttttcttctg gagcatttgc tagctcttca attgtaggaa accattccat gaaacgataa
     1681 aaataatcga tcactgtgtc tactctggtt tgctgaagca tgatctcaga gatccagata
     1741 cgataaggat cacgattata gcgccaaggc aggtttcttt tttcttgttc ataccattga
     1801 atgaactgat cttgaaattc ttttgtttct ttatctgtcc atttttccca ttctttattc
     1861 actttcctct tccttttccg taatgaatcg ctggatcata tccaaatcaa atcctttttg
     1921 atataagctt tgctttactt ttagatttct ttttttaggt tcaaacctta aatttcttcg
     1981 ccatagttta tctccttgtt tgactaaata atcatattct atttcttgat ctgcttcttg
     2041 cggaagatca gctatcgctt gttggatcac ttcttgagaa aagcctttcg tcatcaatgt
     2101 ttgttggatt tttctcaata attctttttg gctttttccg tgattttttc caaacgcttt
     2161 ttcggctgtc tgatgtgcat tttgaacttg gtcttcaaat gtatattggc tgagcgcttg
     2221 ttcaatgatt tcaggtttca aacctttttg ctgcatctgc tgcgctaatt tctttggtcc
     2281 tttgtcactt aagcgcatat tcgtgcgcac ataactttcc ccatagacca aatcatcgac
     2341 aaggtccaat tcttttaagc gggctacgat tttatttcga tcttcttgaa ggatttcctt
     2401 ttcttttaag tatgttcgga tttctttttc cgatcgcaac tgataactta agtaattcat
     2461 tgctaggtga aaaccgaaat cagctccgct actcttcttg atctcttgga tagtttcttc
     2521 gtctaattcc cgtcctttta ataatcgata gcgtactaat acatcttcag atacttgtaa
     2581 agcttctcct tcagataaat ctacgcgata gaaagggcct ttgcctttac tgattctcgt
     2641 aacggttaac ataaacatcc tcctcgaaca tatattctct atcataccac agaaaatccc
     2701 cgtaatcagg tattatatgt tagaataaac cacagaaaaa agaaaaaggg agatacttat
     2761 ggcagaaaca attgtcaaaa ctggacaaac ggttcctttg aaaatcaagc gtttaggcat
     2821 aaatggtgaa ggaatcggtt atttcaaacg gttgatcata tttgttccat atgcactacc
     2881 aaaagaagaa gtactagtga aaatcacgaa agcaactcct cgctatgcag aaggagaatt
     2941 aataaaagtc aaaaaaacaa gcaaagatcg cgtagtggca ccatgtcctg tttactatga
     3001 atgcggtggc tgtcaattac aacacttagc ttatcaagct caattggatt ttaaaaagga
     3061 tctgcttttg caagcattgg aaaaattcaa accagcaggc tttcgatctt atcaactttt
     3121 gccgacgatc gggatggatg agccttggca ttatagaaac aaagcacaat ttcagttacg
     3181 aaaaaataag cagactcaaa aaattgaagc tggtttatac gaagcaaact ctcatcagtt
     3241 agtccctata acagactgct tggttcaaga acctcgaaca caaaaagtaa tgaatactgt
     3301 tgttcaacta ttgaataaat ttgatgcacc catctatgat gaacggacaa acagtggaat
     3361 cttccgcaca atcatggtta gaatcggtgt taaaactggg gagttacaag ttgtttttat
     3421 tactcgcagt aaaaaattcc cgcaaaaaaa cgcaatgatc cgtgagatca atgaacgact
     3481 gcccgaagtt gtttcaatca tgcaaaatgt ccaggcacaa aaaacgtctc tcgtgatggg
     3541 ggatgaaacg atccacttat ggggaaaaga agccattgaa gaacaaatca atgaagtgac
     3601 tttcgatctt tctcctcgtg ccttctttca actcaatcct cagcagaccg aattgcttta
     3661 tggcgaagga atcaaagcat tagatgtcca gcctaatgaa acgatcgtgg atgcttattg
     3721 cggagttggg acgatcggct taagtgtagc aaaacaagcc aaagaagtac ggggaatgga
     3781 taccgttcct caagcaatcg ctgatgcaaa aaaaaatgcg gaaagattag gatatcacaa
     3841 cactcgatat gaagtcggga cagcagagca agttctgcct aaatggttga atgaaggttt
     3901 tcagcctgat ggtatcatcg tagatccacc acgtacgggt ttggataacg cattgatcca
     3961 atcattattg aaataccctc caaaaaaact tgtttatatc tcctgtaatg tttctacact
     4021 tgccagagat ctggttaatt taagtaaaaa gttcaagatt gaatatttac agtccgtcga
     4081 catgtttcct cagactgcac gttgtgaagt tgtcgtgaag atgacgagac tcgctgacta
     4141 aatgaaagag gttgtgaaaa agtctgccct gtatcaagta ataagaacag ccaaacaaaa
     4201 atagctcttc atgttttatg aatactgtac ttgtgaccga agagttaaca ggaggacact
     4261 atttatttaa aaaaagggtt gtgacatagt ttttgtcaca gccctttttg attattattg
     4321 gatattcacg tctatcactt gataaaatgc atttgtcgta tcgctgatat tccatactgc
     4381 taaaatcaca ttatatcctt ttcgatcctg cggaagtgtg atcgtttggt tgactgtttt
     4441 tggtggaaca gttgctttgt cgtctatttt tgtgatcaat tcaaaatcac taaatttcaa
     4501 aggttgatta ggattccaag ttggtttggt aatgtagtaa tcccaagtac ttgtccgatg
     4561 ttgtgctgtt aaattccaag taatatttaa tgttcctgga ttaaccatgt ttttatgcca
     4621 tctgttagat gtctgttcat ctaaaggctc aaagccgctg actcctgcac tagcaatttt
     4681 tccatcaatg aatgtatttt tggtagcttc gatactttgc ggttcccatt gtgctcttcc
     4741 gacattttga tttaagtttc ctgctgctga tgttcctagg tatgcacggc ttcctggaga
     4801 agtaatataa ccatgcgcaa aacctggaat gctaaaaccg aacaacccta aactaactaa
     4861 cccaataaac aataacgtac taatcttttt catgtagaaa cctccttcat ttgataacca
     4921 cattgtaaac gctttattgt attaacacaa acattatgct tttataattt tatgttgaag
     4981 taaaaaaggt gaaaaaagta agatgcagat tataacagaa gtagacgaga aaagtacaaa
     5041 tagaaaacat gagaatcaaa tcatatgatc tttttcaaaa acatcattca tataaacaaa
     5101 tgttcaactt tttaataaca acaattatca atttttttct tacttcacaa actaaataca
     5161 ggtcttgctg tgagccttca tggtaaacta atattataga aaggagttag taaaatgaag
     5221 atgataaaag atattcgttt ttgggtttgt gttataggaa ttgtcatatt aggcttttta
     5281 tcagggttgc ttagcggaaa tcccggaaaa tattactatt ctttgcaact tccaccattt
     5341 gcaccaccta gctggatatt tgggccgatg tggaccttac tgtatatatt gatgggtatt
     5401 tctctttatt tgttacttaa tcacaacaac aaaaaacagc gaaacaatct tgtcggtctt
     5461 tttgtgattc agttcatttt taattttata tggtctgctc tattttttaa tttaagaaat
     5521 attttcattg cagcaatcga tattacactt ttagttattt tcttgtcagt gcttatgtat
     5581 cagttgtggc tgcatcatcg cctgacaatg tggctcatga ttccttatta tttatgggta
     5641 ctattcgcaa ctctattgaa ttattcaatt tattttttaa actaagcgct aaaaagagac
     5701 gtcctgtaat ttaacacata gaaaaagtgc cacctaagtt agattttttg tctaatttag
     5761 gtggcactct ttattaacca aactattttt ttctagtacc cacttgttgt tccatactca
     5821 ttattctggt cataaccata accattttgt tcctgtccag cgtaaggatc ataactttga
     5881 gtatcttgac ttggtgtata gtactgactt gtatcttctg tttgctgata ataggtattc
     5941 ggatcttggt actgatcata tgtgttttct gaataggaag aataatcggt gtacccttca
     6001 ctattcgatc catcaccgtt tgtcgaatcg ccatcttccg aatcatcaaa gaacatcatt
     6061 gaagcattct catttttcaa attaggtaat gtatcgcttg tttttatatt taattgtttc
     6121 ttcagttcat tttggatcga aagaagatca ttttttccta aaatttgata gtagatatca
     6181 ttgatcattt gtccttcgcc ttgcaactgg tcttgtttga ttgtttcaaa cgccggggtg
     6241 tagttcgtcg ctacatcaag catgtcatcc caatctaagt ccgtcgtcac attgttctca
     6301 actgctttta aaatttttct ataattgctt acgccatcta aacttaaaac tttattgacg
     6361 attttcgtca ctacttcccg ctgacgacgt tgtcgaccga tatcgccttc tggatcttcg
     6421 tgtcgcattc gagaataggc taaacctttt tctccattca atgtttgttc acctactggt
     6481 acatggacac catccaaagt aaagtcaatt ttattattca ctttgatgcc accgaccgca
     6541 ttgatcaaat cttccatgcc atccaaattg atcgttacat aatgatcaat aggaatatcg
     6601 agcagttgct caattgaatc catggccatt tcgacaccac caaatgcgta ggcatgattt
     6661 aatttatcta ccgttccata acctacgata ttcgtataaa tatctcggtc caaactgatg
     6721 atcgttgatt ttttttgttt cggattaact gttactacca tcatcgtatc agaacgtcct
     6781 tgttccgttc ttcctaatcc accggtatct aaacctaata gcaaaaccga aaaaggttct
     6841 ttgtcatcaa tgcttactct ctcagaacgt ttggaggata ttcgattaga gtttttagaa
     6901 attttgttta ctgtttggct cgcttctccc ataaatctga ttccgtatgc tgataatccg
     6961 gcaatcagca aaacgaatac ccctaacaag gttaatagaa ctttttgcca tcgcttcata
     7021 atacacctct gtttcacatt ttctaaaata ttgtctcata taactgttca atcagcaaat
     7081 tcacttttta atcataagaa aactttaaaa aacatttttg tgtaacgatt attatttttt
     7141 aaaatttctc aaaattctta actatcatac cataccgtga aaaagatcga aagaccgaat
     7201 acacgcagtt cgcaatcgtt ttatcttttt ttatgtattt tttccagaaa aagcgaacaa
     7261 caaggtattt tccaaataat cgtgcagtaa aatgatcgca catacctttc ccatcttttt
     7321 aacagtcttt gcctaccgtt cagattatca ttcgatttca cgacaatttc ttcctatgac
     7381 tggtcctttt gttaaaccca tcttctattc gttttatttc cagctcactg catggctctt
     7441 aaaatgactc gaactagtga ttgcattacc atcatctttt aatggataga tatgctcctc
     7501 ttcaaagtaa aacgattttc ttttaaagta atagaattga catggtaatg agtatgtata
     7561 ctagtggttt cacaaatggt cttttattca acatccaaaa actgaaattt ctttaaaatc
     7621 cattactaac gaagaaaatg ttccttgctt gataattagt taggacatgt caagtgaaca
     7681 aatgcattta cttagccaaa aaatctaaaa acttcataaa tagacaaaaa gacacgactc
     7741 tctaagaagt cgtgtcttta ttcaacaaat tattataatt tagtcacttc agcagcttga
     7801 ggtccgcgtg caccttcaac aatcgtgaat tctacttctt gaccttcttc taggctcttg
     7861 aagccgtctc cttgaattgc tgagaaatga acaaatacgt catctccgcc gtctactgta
     7921 ataaacccaa atcctttttc attgttgaac catttaaccg ttccgtgttc cataaatgta
     7981 tatcctccaa aaaagttctt gcaagtaacc ttgcaagaac attataccca atttagaaaa
     8041 cgctagcaag ctttcgctct tttattttcc aaattatttt taagactgat atgacggagg
     8101 aaatggttat tttgctgctt cgaaacgttt gtttacttca tcccaattaa taacattcca
     8161 gaatgctgca atataatctg gtcggacatt tttatatttc aaataataag catgttccca
     8221 aacgtccaaa cctagaattg gtgtctttcc ttccatcaat ggagaatctt gatttgcagt
     8281 agaggtaata gctaattttc cattttccat tacaagccaa gcccatccag aaccaaatcg
     8341 tccagcggct gcttttttga attcttcttt aaaagaagaa aaatcaccaa aagcttcatt
     8401 aatcgcttct tttatttctc ctgtaggttc gccaccagca tttggtgcca tgatttccca
     8461 gaaaaatgaa tggttagcat gtccgccacc attgttacgt acagctgtct tgatatctgt
     8521 tggaatagcg tccatatcag ataataattc ttctattgtt ttttcgccta attctgggta
     8581 tttctcaatt gctgaattta aattcgtcac ataagtattg tgatgcttat catgatgcag
     8641 atgcatcgtt tcttcgtcaa tataaggttc caatgcgtca tatgcataag gtagatctgg
     8701 taatgtgtaa gtcataaaaa aattcctcct caatgttttc ttaataatgt ctacattaca
     8761 aagagtaaca aagagaagga aattcttcaa ttgttatgcc tagtcgtctt tttcactttg
     8821 acctacttct tcttcaaaaa cctcagtttc tttaaaaata aacaacttgc tgaaaatata
     8881 gttcgcaagg ataacaataa tttgagtgat gattttcgcc acatactcat tgctgttcat
     8941 aacattgatc aacaatacca ttgcccccat gtccaatccg taagaaagga tacggtagaa
     9001 gaagaactta ccgcattcaa taagtgcatg tgtaaaattc ggtgattttg aatggaatac
     9061 ccaaagtttg ttcgttacaa aagcaaacaa tacagaacaa aaccacgaaa tagaattgga
     9121 taaggccata cttaaatcaa aatattggcg tgcgagagca aaactaacaa aatttacaat
     9181 cgtagccaaa ccaccaaaaa agagatagac tagtatctcc caatatcctt ttgcttctaa
     9241 atatcctttt aactgtcgat actgcttcat gagccaacct accttattaa aaattcttat
     9301 ttatcatact ctatatttca ataactttca tctttattta aaaaaattaa ttttttttga
     9361 tcctttgcct ttgtctctgc ttggatcgtc acatgtgtaa taccatattt taattttaat
     9421 aaagaaatta tacgaggata tatcttttca atctcgctta gtagctgatc ttcaaaatca
     9481 atatgtccgt cgaaaataat tatcttttcg ctatactgcc aaatatggac atgatgaaca
     9541 tcctttaccc catcgattgc ttcaatttct tgttccattt tttcatagtc gatagcaggt
     9601 gcactttgta tcaaaatagc ggcagctttt ttgatcacca ttatcgcttc tatcaatata
     9661 tatatcgaaa tgatcagtgt aataactgga tcgatccagt agatatcgaa aaaacggatc
     9721 aaaattgctc cgataatcac acttaccgaa gacagtgtat cacttaacaa atgcaaataa
     9781 ctagatttta tattcaaact ttcatctgct tcttgtcgta ataataatac tgagaacaaa
     9841 ttcgcgagca atccaataac tgctactgtt agcatcaaat gactatttat cttttctggt
     9901 gaagtgaacc gcttgaatgc ttcaacggtt aaaaacaagg ataatatgat caatatgaat
     9961 gagttgatga aagcactcaa tatttctaaa cgctggtagc catacgtacg ttttttattt
    10021 gcttcttttc gggccatttt ttgtgccccg taagaaaaaa taattgccaa tgtatcacta
    10081 aggttatgga aagcatctga aacaagagac aaactccctg acataatccc tccaataaat
    10141 tctgataaag tgatcaccgc attcaaaacc actactccaa atagtttttt tgatgatctc
    10201 tgtggctgtg taggcactat tccttcctcc attttattta tattggatag tctttagaca
    10261 agaataacat ttttttgata tttcaccaat cattcagctg tttccttaaa gcacttgtat
    10321 ttcacttgat acttcgctag aattgcaagt gatgacacta ctagatgagg agtacttatg
    10381 acaacaaaac aattaattat cagttccttt atacgattca aagacctaaa ggaggcgaga
    10441 aatgtcccat tttggaaagc cattgtctat cttttagttt tgagtataat tatggctttg
    10501 ccaatctctt ttcaagtttt tcaagtctta aacaacattc aattggatgg gcaaaagatt
    10561 gctactcgca ttcctgattt tacaatccaa aacgggcagc ttcaaaccga agaaaaagaa
    10621 ggattcattt accaaacaaa ctccattatc ttcacatttg atccagaggg aaaacgaaca
    10681 gaacaggaca tctctactga tttgatgggg aattttgtta gtgtcgggat gttaaaaaac
    10741 aaacttatca tagctcttcc taatacaggc acgacttctg ctttgttgaa caataaccaa
    10801 ttagaacttc cctacaccaa tgaatcttta aaaaacttaa caggtaagca gctccgttct
    10861 tttttaagtg aagcatctat accactttgg ataaaagtcc tgacatttat attttctatt
    10921 tatcctagtt ttctgaatct gctgatcact ttgctattta ccaacattgc tgcttttctc
    10981 tatgcacgct ttcgactaac aaaagctact tttcttgatt gtttaaaaac attgatctat
    11041 tcaatttcat tgcctactat aattgcaaca atcttgatgg tctttcttcc ttcttttgac
    11101 actagcgcct tcattgcttt tgcaggaatc tttatttttg ctcaagctgt caaaggatgg
    11161 gcaaaaatat cgatcaatta agaaataaag acaactcgtt atttctactt caatagaccc
    11221 ttttagaggt tgtgacattt ttttgtcaca gccccttttt tatttgaaaa atatttcttt
    11281 ctcaaaaaac aagcaaggcg cttcaaagtt cttcttgtat gcgctacact tagagtaaag
    11341 aaaggcggga aattaactat gtcagaaaga atcatttatg cagatcgtcc atttcgtgat
    11401 gaatttatcg aagaaatcca tactatagcg cctggttata tctttaaaac agaattacaa
    11461 ccagaggatc tacaaaatgt agagatcagt ttgggctgga actcagacta ccaagaaaaa
    11521 ctattagcct cttctacctt gaaatgggtc caatctatct cagctggtgt tgatcagcta
    11581 ccccttgaga ctttttcaaa gaaacatatt ttactttcaa atggtagtgg catccacagt
    11641 gaatcgatta cggagcatgt cttaggaatc atccttgggt attctcgtgg tctttttcaa
    11701 gcacaaaagg cacagagtga catgaaatgg ttaggttcat cggtccatta ccaatctatc
    11761 caagggaaaa aaatactaat cattggaact ggccatattg gaaaaatgat tgcaaaaaaa
    11821 tgcaaagcat tagacatgaa ctgctatgga atcaatacat ccggtcatcc agcagaagga
    11881 atggaaaaga ctttttcttt aaatcaacta aaggaagttc ttcctgaagt agatttcgta
    11941 gtcaatatct tacctctaac ggaggaaaca actgggctct ttgatcagca tatgtttgaa
    12001 cactttgatc caaaatctgt attcatcaat gttggccgtg gtgcttctgt taagacacaa
    12061 gatctggtgc aggctttaaa taaccagcaa ctttcctttg cagcattaga cgtttttgag
    12121 gaagagcctc ttccacaaga tcatcctctt tggaaaatgg acaatgtact gattacttcc
    12181 cacattgcag gtttgacacc tgattttcaa aagaaattaa tggctatttt tttaaccaat
    12241 ctgaaaagct actttgccac ccatgagctt caaaccaatc aagtaaaatt gtctgcagga
    12301 tattaatagc tgaaaacaat attttcaaaa gaaaaaagag aaggtgacaa aaattgtgtc
    12361 accctctctt tttttattgt tttttaaagt gataggttgg ctcctccagg ttgggatcat
    12421 aatcaacaag taaatcatat cctttaaaga accattcatc tgcttcctcg ataaaaaaaa
    12481 gtgtcccatc catttccact ttctttacag gatgttctgg ctgttcaact gacattccaa
    12541 tcgaaaaacc atcatgaacc tctgttttgc cgtagacttt tccaaaaaaa cgtattccgt
    12601 aagtttctgg cagatcaatt tctgagtcaa accacttttg tgcttttggc gttaccgtta
    12661 atttcatccc aacaacctac tttctctttt ctttctattg attcatctta tcgtctagaa
    12721 tatccacatc gcctgtctct ccaataacca gtagattatc attttcacga acatattcat
    12781 ctgctgcagg agaagcgata accgtttgat ctactcttcg tatggcgact actgttaagt
    12841 caaaacgttg cctgaaattt aattcagcta atgttttgtt gaaaaattta ggattggaga
    12901 ctcggatttc tgctaaagaa tatttatctg acaactctag gtaatctaaa atattctttg
    12961 acactaaatt atgcgcaatc cttattccca tgtctcgctc gggatggacg acgcgatcag
    13021 ctccgatctt ttcaagtact ctggcatgat actcattttg ggcttttgct aaaatatttg
    13081 gtacgcccat ttcctttacc atcagagtaa ctaagatact ggcttgaata tcttctccaa
    13141 tagcgacaat tacatgatcg aaatttctta tgcctaatga tcgaagtgtc atttcatcct
    13201 gtgcatttgc cacaacagca tgagtggcta tattcatata ttcattgacc cgatcttcat
    13261 tactatcaat agccagtact tcttgaccag cttcgatcag tgttcggcag atacttccgc
    13321 caaatcgtcc aagacctaca attgcaaaat tttgtttcac cgttttcctc ctttagtatc
    13381 ttcttttctc agtataacaa agcctttaca aaaacaaaag caagcatcat tattgtaaaa
    13441 aagccaagaa aaataatagt tattttctca gcttttgatt atcctcttta tcatcgtttt
    13501 tatttcacca aaccatgttt gaatgcatag atagcggctt gtgtacggtc ttccacttct
    13561 agcttagcca atatattgga aacatgtgtt tttactgttt tcagtgtaat aaacaattcg
    13621 tccgctattt cctgattact ttttccttca gaaatcagca ttaaaatttc attttcccga
    13681 ttcgttagtt cttcatgtaa tacaggctca tttcgccgac tcattttttc catcattttt
    13741 gttgtcactt ctggttctaa tactcgctcg ccacgttgcg ttgcccttat agcatcagca
    13801 atttcatgtg cggtagaggt cttcaacaaa taccctgctg cacctgcttc aatggcagga
    13861 taaacttttt cgtcatcaat aaaactggtt acgataatga ttttagcttc tggccaatct
    13921 tttaagattg cttttgttga ttcgatacca tccatttctt ccatgactaa gtccattaaa
    13981 atgacgtccg gtcgaagtgt catcgccttc tcatagcctt gtcgtccatt ttcagcttct
    14041 ccaattactt caatgtcttc ttgaatagat aaataagaag agacgcctaa gcgcaccatt
    14101 tcatggtcat ctactaataa aacttttatc atccttatct atgcctcctt catcaaaggg
    14161 accttgattt ctacacttgt accttgacct ttaaaactaa tgatttttac tgttccacca
    14221 ataccagcta ctcgttcttt gatattgttc aatccataac ttcccgtttt cgtttcattc
    14281 atattaaacc ctgttccgtc gtcaataata cgtaacaaaa gattattgtc tattttgtgc
    14341 aaatatacct ctaattcatt ggctttagca tgtcttaatg tatttgataa caattcttga
    14401 acgattcgga acaagtgatc ctcaatggaa ctagatagtt ttacatcttc tacatcccat
    14461 ttcagcgaaa tctggatttt attttgcaat tccttcaata actgctcgat accttgcttt
    14521 aaacttttct cttctaaatt gactggacgt agatgaagaa gcagcgcacg catctcagac
    14581 tgggaagcgt tgatgatctc tgctaccatt ttcaactgct tgcgaaacat ttctggtgtc
    14641 tcgctttttt ctgcttgctc cgtcaatgcc gacatcatca tcattgctgc aaacaattgt
    14701 tgcgaaactg aatcatgcag ctcacgggct aatcgatgtc tttctaattc taaaatttct
    14761 tctttggttt gtccatctac atattgggga cggctcgtta caatttgtaa ttcactagat
    14821 acttcaatca ttttttcttt tatcttagtg atttccttat caatatcttt gatgtataaa
    14881 tcgtccgatg cacttgggat cggaagaagc aacaatttgg attcataatt gccagaagaa
    14941 agtgcacgca gcttttcttc gattttcccg tattttgtct tttgaataag tgataataat
    15001 agaaagacaa ttaatcctac acctaaggaa atggctaaca aatggaagat aagcggaaca
    15061 tataataaac gtgcacgcag taattctccc caccaatgac tttgattgct ggcataaaaa
    15121 taagtgaaaa gtgaaaatag aataataagg aaagccgcaa tccccgaata aacagctagc
    15181 atcgctctgg atatttttcc catcatattc gtatcacctc gacatctcca atcaaggtat
    15241 tagtaataat cttcaatcta cgagggtttt catcataatc actgctgtag attttgattt
    15301 gttcattttt caaagaaaat tgctcttctt caaacaagac atttcctact aatgtcgcat
    15361 gctctaatcg gatggctacg cccaaaggta ctaagatccg tgttctgcct atcccttttc
    15421 ttacaatcac aatattatca tcttttggca aaagcgtatt gcccaaatct atgatagtat
    15481 caccagaaat gacggcgatg ttgatatcgt cccattcata gacgtcgttt ccaatacgtt
    15541 ggttaccgaa taattgctgt cttttccgct cattattatg tgtttttaat tgctctgttt
    15601 ggatcatcat gatctgtttc tttcgccaaa aagcattttt ggttaaatct atccctgaaa
    15661 tctctacacc tttcagacca ataaataaaa cggcaaatac agccatcatc cataaagcag
    15721 gactgttgac taagctaaaa aaaatgacca aacttcctaa tatcaattga aaattttgaa
    15781 atttagtacg aggatgtttg cgcatagcta gatatatatt gaaaataccg aatatcacta
    15841 aaagcaaaag tccagtattg tttacgattt gccaaacagc aaataacagc agtagtgctt
    15901 caactaccac aaaaaaacgc caagaactat tcatcgctct ttctccctcc tgacacattc
    15961 tcatttcatc atacctattt ttttaactgt tggctatagg actttagatg taagatcgtt
    16021 tgttgaaatc taaaatacac aaagagaaaa agatctagag tgtatcatca gtaagatgaa
    16081 ctctctagat ctttcagtca gactacgctt gttctacttt gacgatttta actaacatat
    16141 cgccacctgg agtagcaata gttacttcat catttttacg tttgccaatc aatgcttgcg
    16201 caataggaga atcattggaa attttgccag aaaacggatc tgcttcagcg cttccgacaa
    16261 tcgtgtactc ttcttcttct ccatctggta gttcgatgaa tgtaactgtt ttaccaattg
    16321 atacctcatc tgcatctaca ccgtcgttat cgatgatttg agcaaaacga atcatatttt
    16381 caattgttgt gattcttcct tcaacaaatg cttgttcatc tttcgctgat tcatattcag
    16441 agttttcgga aagatctccg aaaccgcggg caattttgat tcgttcaatg atttctccgc
    16501 gtttgactgt ttttagttct tctaattctt tttctaattt ttctttacct tctagagtca
    16561 ttggataaac tttctctacc atgtgtaacg tctccttttt tcgataaaat accgcaaaag
    16621 gtaatctttt gaggaatata gtccaaaaaa gattaccacg cttttcttca aatgtaaatg
    16681 tttttctaaa aatgatcaat gtctagagcc tgtttttcta agaattattt acatattttt
    16741 ccactaattc catatgttct tcataagttt tagcgaaata aacatttcca gtgctcgtat
    16801 ctgctacgaa ataataatag tcattttctg ctggttctaa tactgccttg attgcctctt
    16861 cgcttggatt gtcaaaaggt ccaggtccat agccggtatt ggtatacaaa ttatatggtg
    16921 aatctacagc tgtatcttca tacgtcacta attctttgtg ttcacctaat gcgtataaaa
    16981 cagagatatc tgactgaagt ggcatatttg ctttgattcg gttaaagaag acttgtgcaa
    17041 tgtttttacg atcattttct tttacgcctt ctttttcaac tagagaagct agagttaaaa
    17101 cttcttgtac agtcaaattt ttctgcttga tagctgaata atagttttgc atgaccgtat
    17161 tcgtcttgtt caccatttca atcactagat ccttaagcga agtactcgtg taataatcat
    17221 atgtggctgg gaataagtaa ccttccaaac ggtattttgt gttctcagct ttagatgcac
    17281 tttccaacag ttctgggaat ttttggtgca actcgttaaa gaaagtctca tcattcacta
    17341 aatctaaaaa ttctttcttg tctttaccag ttaccttcgc tacccgttca gcaatctgat
    17401 cgatatcgta cccttctgga atggcaattt tcccatctgc aacttttgtt ggctcagaag
    17461 taccgccttc ttgcaactgt ttgccaatct cgtcaagagt catattaggc gctaactgat
    17521 aatagccagc ttgaaagcca gttaaatttt taaattttgt ataataattg aaaacaatcc
    17581 cgcttttaat aatattgtcc ttctccaaaa tctcaccaat ctgcttgtta gaagagccac
    17641 tcgggatctc gacttggact aattggtcat ctgatttatc taaaggcttc aacccgctat
    17701 ctacataacg atacacggta aatccaagaa atccgccaat aatcaataat gtaagagcaa
    17761 tcactaaaat gatcttcctc acaatcttat cttctttttt acgcagattt ctccgcttgt
    17821 cgattatatc tgctgtagct gccttactgg cttctttttt atctttactt gggtgttgtt
    17881 tgtttcctga caaaacaact cctccttact atcaagttcg taactttatt cattatacat
    17941 gatatttgaa ttggcgtgaa tgattttatt taaatattct atcaaaaatg taagtagtaa
    18001 atccattact ataagattaa aataaatgtt atcaagatac aagctatttt agctttattc
    18061 tttctgtaaa aaagaaagga gactaaataa actattatga aaaaaggaaa acatgctaaa
    18121 aaaagaaaat attcattttt aatgatcgtt gtcgtgttgt ttagccttag tgcgttctct
    18181 acaggaacta ttcttggatg gcaattttac aataatgaac actctcagat agttgcccat
    18241 gctcaatcag ctcaaaaaaa gttgatactg actaccaatg aacaacagct cgttgaaaaa
    18301 atcgatcaac tattaagaaa aaatgattat attggctcaa tttacgtaaa gaaaaataat
    18361 cgcatcatca tggaaaaagg ctatggctat gcgaataaac tcacgcaaca accaaatgat
    18421 ccttctcttt attatcagat tggttcggtt caaaaagcat tgactactct tttgatttta
    18481 aaacaagtcg agcgtgggca cctttcttta gatactaagc tggctcaatt ctatccacaa
    18541 attgctggca gccagcaaat tactatccgt gacttgctgt atatgcgctc aggcttgcac
    18601 cgaactgctt ctccaaccat accgatgact gatgaagaag tcgttcaatt tgcgattcag
    18661 cacttaaaat ttacagatta tcagacctct cattatgatt cattgaactt taccatattg
    18721 actggaattt tgatcaaatt aacaaatcaa ccgtatgaaa aattattgac cgatgatatc
    18781 atcgaacctt taggattaca gcatacaaaa ttttatgacc aagtcaaaaa ttcatccaat
    18841 catgcactat cctatcaaat gtctaaagag gatgattact ttaaaccact gacagaatct
    18901 gaaacagaga tccataacga attagggact ggtaatatca gtatgtccgt ctatgattta
    18961 aatacgctgt ttacacaagt tttatctgga aaactagttt ccaaagactt actcttctca
    19021 ttatggcgtg aaaatgcgaa tggacatcca tatagtggtg gggtatatag cggagataat
    19081 tatatcctgt cgcaaggaaa tatcgactct tttgatacat ctgctatatt tagcaaagat
    19141 tcacaggatg ctatcatcat ggaaagcaat ataaaagctg acaaaactat caaactgcct
    19201 actgctgatc taagaaacca aatctatgac ctgatggaag gaactactaa gttggcaaat
    19261 agctgatatg atgaaaagat agcaaatact acttctttga atagcatttg cttaaaaaaa
    19321 gtactcgact aaacgtagga aagagtgtgg gacaaatcta aaaatcaatt tttgctacag
    19381 actttaattc caaataaaca gaacagaaag aggtgttctt ctttctgttc tgtccaaatt
    19441 attctaagat ttctctttac caacattatt tgacaaaggg cctattaaat ctaaaatagt
    19501 atctttagat tgctaaaata tgtgctagca aatcattcat ggcttgttct tatttttaat
    19561 tttatgcaat aaaaaagaag aacaattagc gtgattatag aaataaataa tgagatttta
    19621 gaaaaaaatg tgtatgaata cctgattgtt actttattta ttccactttc actttttact
    19681 gtaacaaaag gacctccttg ttttagtact gtagcatttt ttgaattaac agtaaccgtt
    19741 aatcctttat accggaatac aggtaaaact attgttttgc cttttgaaga tttaaacgta
    19801 aatgtaattt catctttatt tggagaaaag ctactataaa taacttgacc atcttctgta
    19861 acttgatggt ttttcaaaag caattgatct tttttatagc ctataggtac ataatcaaaa
    19921 ttctcaaatt cttcaagttt attttccatt tgttcattag aataagttcc ttgaaaattc
    19981 atttctttca cca
//
LOCUS       DARBET010000047        19821 bp    DNA     linear   BCT 07-NOV-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4027
            SAMEA114569841-rid20112183.denovo.047, whole genome shotgun
            sequence.
ACCESSION   DARBET010000047 DARBET010000000
VERSION     DARBET010000047.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA114569841
            Sequence Read Archive: ERR12203666
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 19821)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 19821)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001972618.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001972618.1
            Genome Coverage       :: 132x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/07/2023 13:18:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,041
            CDSs (total)                      :: 2,975
            Genes (coding)                    :: 2,908
            CDSs (with protein)               :: 2,908
            Genes (RNA)                       :: 66
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 67
            CDSs (without protein)            :: 67
            Pseudo Genes (ambiguous residues) :: 0 of 67
            Pseudo Genes (frameshifted)       :: 26 of 67
            Pseudo Genes (incomplete)         :: 28 of 67
            Pseudo Genes (internal stop)      :: 20 of 67
            Pseudo Genes (multiple problems)  :: 6 of 67
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19821
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA114569841-rid20112183.denovo.047"
                     /isolate="VRE4027"
                     /db_xref="taxon:1352"
                     /country="Denmark"
     gene            6..305
                     /locus_tag="SCJ86_001851"
     CDS             6..305
                     /locus_tag="SCJ86_001851"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288285.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HEG1169389.1"
                     /translation="MKNRLIVACGSGVATSQTIASKIQSMLEDDGIAFPVEAVDYKSI
                     QNELLTAGIYVYVAQPDEEVLEQAKDLGIEVFPGIPFLTGMGVEPIYDSIKELVQ"
     gene            378..1739
                     /locus_tag="SCJ86_001852"
     CDS             378..1739
                     /locus_tag="SCJ86_001852"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629627.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS galactitol transporter subunit IIC"
                     /protein_id="HEG1169390.1"
                     /translation="MEVFQTIINYVLNLGSAIFVPLIILILGLLAGMKLKKAFMSALT
                     LGIAFSGMSMVIGYMSNAVSPASEALAKNTGISLPALDLGWTGAASITWSWSYAFIFF
                     AVTIGVNFVLLLFNWTKTLNVDMWNVWGKALTAYLVYYVSGSLAAGFLTAMVQVILEL
                     KLGDMFQKHIQDLTGIPLVTVTHFMTSAAVLLLPFNMIMDKIPALNKRADTNALKKKI
                     GIFSENSVMGFIIGLGLGFAAAYGVSGSLNLAIQTATALSLFPMISKMFMQSLSPLAD
                     AMSEFMKKRFKDREVYIGLDWPILAGRSEIWVTAILLVPVFIGYAIILPGNQVLPLAG
                     IINYSIAVGGLLLTGGNLARMLILGIITMPIYLYGATYLAPILSDLAEKSGAVEGIKK
                     GQLITWSSIEGPEFRVLFAEAFNRNILAIVGCVIFLGLFVLLYKYMIKAKVPSERYAE
                     LQK"
     gene            1761..2021
                     /locus_tag="SCJ86_001853"
     CDS             1761..2021
                     /locus_tag="SCJ86_001853"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377613.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEG1169391.1"
                     /translation="MKREETDKIKWTVALCGTLLLFLYGLFTQNIIINLLVIFFALVI
                     YKYGNHVLFREYDEKRKRKIEESMKIKEATKEILREKSFIKR"
     gene            2035..2787
                     /locus_tag="SCJ86_001854"
     CDS             2035..2787
                     /locus_tag="SCJ86_001854"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288278.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class II aldolase/adducin family protein"
                     /protein_id="HEG1169392.1"
                     /translation="MSDGRMLFERERQDMARIVRVIFERKNTNVAGGNFSFKTTDDQG
                     KDYIIMTPTMMSEAYLGEISPAQVLVVEPRTRRIVDGVGHLTREINMHEAMYETNPAI
                     KAVLHAHAPNAMFWATTGLPMPNLTEATAKVKEIPVMDFEPNCSEELAELTSDYVKKQ
                     HLELPHMLLLNSHGVLINTGGESGIEAIHKALQILDTVEWNAEIAYKQTIFQALGITD
                     GFYSKGEKIGGLEELKHKTAIYNKSVIAAGGD"
     gene            complement(2881..3486)
                     /locus_tag="SCJ86_001855"
     CDS             complement(2881..3486)
                     /locus_tag="SCJ86_001855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HEG1169393.1"
                     /translation="MTYIFEKGTSGKHLILLHGTGGDEHSLLDIAHFLAPNSTLLSFR
                     GTVQEDGMNRFFKRNGLNQFDLDSLEKETDKLHEKIKEISEKELIPLSDWLLVGYSNG
                     ANIAAHLLLERETELNKGLFFHPMSLGVHQSRSTLEDKTVWISYGDNDPIVSPASFKE
                     LADQFETRQANVTIQLTVTGHQLTMDEIKQAREWTDHLLTK"
     gene            complement(3539..4513)
                     /locus_tag="SCJ86_001856"
     CDS             complement(3539..4513)
                     /locus_tag="SCJ86_001856"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288275.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ring-cleaving dioxygenase"
                     /protein_id="HEG1169394.1"
                     /translation="MDTQIRGIHHVTAMTSSATKIYRFFTDILGMRLIKKTVNQDDIE
                     TYHLYFTDDTGAPGTDMTFFDFPNQPKGKKGTDTISRASFRVPSDAALDFWLERFEKY
                     DVTHSAINERFGKKYIEFEDFDNQYYQLISDENNQGVAAGIPWKNSNVPTEFAITGLG
                     PVFVRVSQLEHIRLILEKILGFKETDHSGDFYLFEIGEGGNGASIIIEHRTDLPKAIE
                     GFGNIHHLALRVADKEALEYWIQVINKIELPNSGFVDRFYFQSEYFLAAHHVLFELAT
                     DGPGFLEDEPAESAGEKLSLPPFLEERREAIEAYVRPFDTSDANKKRE"
     gene            complement(4891..5610)
                     /locus_tag="SCJ86_001857"
     CDS             complement(4891..5610)
                     /locus_tag="SCJ86_001857"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342701.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HEG1169395.1"
                     /translation="MPKYEEIANILRERIRSKEYPLDSFLPNQVDLVKEFGASRMTIK
                     KAINILAMEGLVYSQRGSGTKILNHPFMQKDTSPANEYEGLSLQMKRRHRSLKSQIIH
                     FDVEFPNQYIQERLMIDAEQPVYNIHRLRILDGEPYVLEHTFMPVHLVPGLKKEHLLA
                     SIYDHLHQELNVHFAGAYRAISADKSDEYDQQYLNCAENDPVLEVQQVVYLKDGQPIE
                     YSSSRNRFDVRNYSVLDVRST"
     gene            complement(5737..7008)
                     /gene="celB"
                     /locus_tag="SCJ86_001858"
     CDS             complement(5737..7008)
                     /gene="celB"
                     /locus_tag="SCJ86_001858"
                     /EC_number="2.7.1.205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002372981.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS cellobiose transporter subunit IIC"
                     /protein_id="HEG1169396.1"
                     /translation="MNGFMDKLSEKIMPLANLLGQNRYLTVLRDAFMLSFPLTMFGSI
                     VVVINNLPFFSDATKGTLSNLFGNGQNATMSIMSVFVTFGIGYYLSKSYDVEGIFGGA
                     VSFASFLILTPFAMKTAGGEDVTGVLSLDRLGAKGMFIGMIAAFLAGEIYCRITKRGW
                     QIKMPDGVPPAVTKSFAALIPAVVTLTIFLVINAVMTGIFNANLHDVVYEVIQKPLTG
                     LGSSLPATLIALFFVQFLWFFGLHGQIIVNSVMDPIWNTLMLDNLEAYQHGKELPHII
                     TKPFMETFTVGIGGSGMTLAVVLLMAFVLKKKQYRDVGRLALAPGIFNVNEPAIFGLP
                     IVLNATILIPWIVAPLIVTTFNYLVMAAGLVPAPTGVSVPWTVPIIASGVLATNSWLG
                     GMLQVIDFVIVAFIWYPFLKVLDKQPDLDIA"
     gene            7161..8558
                     /locus_tag="SCJ86_001859"
     CDS             7161..8558
                     /locus_tag="SCJ86_001859"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 1 protein"
                     /protein_id="HEG1169397.1"
                     /translation="MKTYTFPENFWWGSAASGPQTEGRVSDDGKGENIWDHWYELEPE
                     KFFDQVGPEKTSQVYTKYKEDVQLMKETGHNTFRTSIQWSHLIPEGTGEINQKAVSFY
                     RDYFTELLDNGIEPFVNLYHFDMPLPLQEKGGWLSRETVDAYVSYAAACFELFGDLVK
                     KWFTHNEPIVPVEGGYLYQFHYPNHINMKEAVQVGYHENLASAKVIKAYHEGGHDGEI
                     GIILNLTPSYPRDETNPEDVKAAKIADAFFNRSFLDPAVKGEFPQELIEIVKELAIMP
                     EIHEGDLELIRENTIDLLGINYYQPRRIKAKETPINHEHSPMPDDYFDNYDMPNKKMN
                     PYRGWEIYEKGIYDILTNTRENYGNIKCYISENGMGVEGEERFVNSEGIIEDDYRIEF
                     VTDHLKYVHQAIQEGTNCVGYHMWTCMDNWSWTNAYKNRYGFIAVDLAQDGKRTIKKS
                     GRWFKTVSEKNGFDA"
     gene            8639..9079
                     /locus_tag="SCJ86_001860"
     CDS             8639..9079
                     /locus_tag="SCJ86_001860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340577.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein"
                     /protein_id="HEG1169398.1"
                     /translation="MVDTYRNILVAIDGSEKAEKAFLEAITIAKRNQATLHILYVNEV
                     TGNYFGDFAFVTTSLQEELDEVAENQMKEHRNLAIEKGLTDIETYVLYGYPKTLIANF
                     HESKEPIDLIVMGATGLNAVERALVGSTTSYVVNHASCNVMVVK"
     gene            9227..9553
                     /locus_tag="SCJ86_001861"
     CDS             9227..9553
                     /locus_tag="SCJ86_001861"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289468.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PadR family transcriptional regulator"
                     /protein_id="HEG1169399.1"
                     /translation="MISSDGIRGYNDAIILSILNKNDSYGYEISKTIRERTNDSYVIK
                     ETTLYSVFARLEKNQLIVSYPGEVTHGKKRTYYKITEIGRNLLEEKKQEWLTTKDVVD
                     QFLEGN"
     gene            9554..10303
                     /locus_tag="SCJ86_001862"
     CDS             9554..10303
                     /locus_tag="SCJ86_001862"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016922394.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEG1169400.1"
                     /translation="MDTIRNYLDSLFIGVPQSTEIDKLKTDLLANMEDHYHELMGEGK
                     NEQEAIGTVISTFGSIDELLEELDVEKKHQADETETNTASIYLSEAENYWKEYRAASL
                     QVASGVLFISLSFASFLFFCSAGYVFMGISCLIFGIALAVGFFIASGMKITRLNHFLH
                     HRKIPEKVLAEAKEKEEEYQRSFGFSLIAGIGLCIFSLFPLLASLMWYMDGSIGASIF
                     FVTVGTGVFLIIYGSLVRHSYRQFTQSAYYW"
     gene            complement(10383..10838)
                     /locus_tag="SCJ86_001863"
     CDS             complement(10383..10838)
                     /locus_tag="SCJ86_001863"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289471.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Spx/MgsR family RNA polymerase-binding
                     regulatory protein"
                     /protein_id="HEG1169401.1"
                     /translation="MLTIYSSRTHSYHLMANWLKDHQMPYRERIFTPKQPLTRKEIFY
                     LLSLTENGFDDLLAKRSIDYKNLKDQIDQCTTSELVDLIVSHFTLLQRPIITDGKKLL
                     VGFNEDSMRLFIPVQWRRSQLKSVYNDFTWVSNLENSTDLDDEFLNYYI"
     gene            complement(11265..11540)
                     /locus_tag="SCJ86_001864"
     CDS             complement(11265..11540)
                     /locus_tag="SCJ86_001864"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348748.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEG1169402.1"
                     /translation="MKLEDKKVILKKIKRLPDQIDGYTICSFKKDRSIKAYTQNTEKG
                     IIYFLHEEGYDTQTFMFTEKKEFHKQMKKLIEKEFPRSHKLYVNQQF"
     gene            11834..12649
                     /gene="thiD"
                     /locus_tag="SCJ86_001865"
     CDS             11834..12649
                     /gene="thiD"
                     /locus_tag="SCJ86_001865"
                     /EC_number="2.7.1.49"
                     /EC_number="2.7.4.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330226.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional hydroxymethylpyrimidine
                     kinase/phosphomethylpyrimidine kinase"
                     /protein_id="HEG1169403.1"
                     /translation="MEDQYVQAMTIAGSDSDGSAGMQADMHTFFTRNVYGVSVMTACV
                     AGNSYGIGASVTLPTDFIDKEFELIAKDFQIRAAKTGMLADSKLIETVVKNYQKYDFG
                     PLVVDPVIVTKHGNLLLEESALQSLKEKLVPLAEVLTPNFYEAEKLTGRTLAAEEDFV
                     QAAKELQDMGAKNVMIKGHHAKNAQQETVNDYVLFESGKSFWLNAPFYPTDRINGTGD
                     SLSACIAAELAKGKTVEQAIRLAKDYVNRAIREEINVGHKYGPINHWAAGELE"
     gene            complement(12978..14429)
                     /gene="cls"
                     /locus_tag="SCJ86_001866"
     CDS             complement(12978..14429)
                     /gene="cls"
                     /locus_tag="SCJ86_001866"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cardiolipin synthase"
                     /protein_id="HEG1169404.1"
                     /translation="MVSSIITALYLLNALIALVAILIKPRDVAAIWAWLLVLFALPGV
                     GFVLYLFFGRGLTDKKKFYLRQSDLKELENFQSFRGDTIEHYDPDMGDKDKQQFVDFF
                     SSLNRMPLTRMNSVTLLTDGQEKLDSLLQDLKKAKHSIHIEYYAFVTDNIGQQVLHVL
                     EEKAAEGVEVRILYDAFGSHGTKAKDFNRLIKNGGHVHTFVTSQRALLRFRLNYHDHR
                     KIVVIDGKISYTGGFNIANQYVNTTKKFGYWRDTHIRIFGAASSLLQLRFLTDWNVSV
                     PEEKKVGYHLNYFFKKADRDESKLADTSIQLVSSGPNNEREQIKLSFIKLITSAKKRV
                     WIQTPYLVPDESVIAALKIATASGVDVKIMIPNKPDHPFIYRATQYYARQLIKENVQI
                     LVYENGFLHAKTLIMDDEICMVGSANQDIRSYRLNFETSAVIYDPEFLEELATQFKED
                     ETHCSSMTTETVKEMSNWLLFKQQISRLFSPIL"
     gene            14770..17436
                     /locus_tag="SCJ86_001867"
     CDS             14770..17436
                     /locus_tag="SCJ86_001867"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331875.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation-translocating P-type ATPase"
                     /protein_id="HEG1169405.1"
                     /translation="MENKEQAQQAVYKETIDSLMQQTQSSTDGLSTTQAKEKLERDGL
                     NQLKEKPKKPTWKLFLETFKDAMVIVLLIVALIQMVMGDYIEFLVIFAVLMLNSIISV
                     VQTKKAEGSLEALKNLSAPDARVIRDGKEKTIPANELVVGDIVLLDAGDYVPADGRLL
                     DAGSLKVDEGMLTGESTSAEKEVTDITSTVPIGDRTNMVFSGTIVTYGRGKFLVTSTG
                     NNTEIGQVAGLLESTTEQVTPLQRGLDKFSKKLSLAILALSLLILGIQLFRIYLGEGT
                     GDMTADIVSAVMFAVAVAVAAIPEALQSIVTIVLSLGTNKMAKRHAIIRKLPAVETLG
                     STSIICTDKTGTLTQNKMTVVDYFLGNGDTGDFTNDPSKWTADERRLIEISVLANDAS
                     ISEDGTKLGDPTEVAFIDFSEKLNQPYQEIRNNYPRQAELPFDSDRKLMSTGHTIDNK
                     NYLFVKGGPDIVFQRSKKVLLNGEVVPMTDELMKKFQEQNEAFSTRALRVLAFAYRPL
                     DNFDLSFEDEDDLILVGLLAMIDPPRAEVTQAVTEARSAGIKTIMITGDHKTTAVAIA
                     KQIGIFEEGNLALTGTELDELTEDQLNEVLEKVTVYARVSPENKIRIVRAWQNKGHIS
                     AMTGDGVNDAPALKQADIGIAMGTGTDVAKDAAAMVLTDDNFASIVSAVEVGRNVFDN
                     IKKAISYLFAGNLGAIIAIIFALVADWSNPFTALQLLFINLVNDSLPAIALGTEKAEP
                     ATMNRPPRDPNSGIFTDKTLISVTYRGVLIGIAVIFAQWLGMQHSPEFGVAMSFSTLI
                     WCRTLQTFPARSNTQTAIQAGFFSNRSVLLAVVVCSALYCLTMIPGLKEVFSIPSAFG
                     LTEVFTCMGIALVTIVLMEITKLIIVRVQGNK"
     gene            17800..18552
                     /locus_tag="SCJ86_001868"
     CDS             17800..18552
                     /locus_tag="SCJ86_001868"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629622.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HEG1169406.1"
                     /translation="MNEKEWDEFAEEYYEIQQESQVTIAEDVKNYLKTEKVLPAETAA
                     DVAGGSGRFLSLAKEINRYDLIDFSSGMLENAKKEAEKNELPNVSFIKQSLEEFIQSN
                     ETYEMVFTAANPALVHLIQLEKLMKKSSKWCVIIRVVESIDDVFYPIEKQLGMIEEDP
                     NTSPVVMDQFETYLIDEGFKVKKKDFTYFTQESITEELMKLYYDAYAKDPVVTRYIRK
                     LFSEKREILSTTKLTYRLLLWSADKKRTFHDS"
     gene            complement(18574..19728)
                     /locus_tag="SCJ86_001869"
     CDS             complement(18574..19728)
                     /locus_tag="SCJ86_001869"
                     /EC_number="2.3.3.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335590.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxymethylglutaryl-CoA synthase"
                     /protein_id="HEG1169407.1"
                     /translation="MKIGIDRLSFFIPNLYLDMTELAESRGDDPAKYHIGIGQDQMAV
                     NRANEDIITLGANAASKIVTEKDRELIDMVIVGTESGIDHSKASAVIIHHLLKIQSFA
                     RSFEVKEACYGGTAALHMAKEYVKNHPERKVLVIASDIARYGLASGGEVTQGVGAVAM
                     MITQNPRILSIEDDSVFLTEDIYDFWRPDYSEFPVVDGPLSNSTYIESFQKVWNRHKE
                     LSGRGLEDYQAIAFHIPYTKMGKKALQSVLDQTDEENQERLMARYEESIRYSRRIGNL
                     YTGSLYLGLTSLLENSKSLQPGDRIGLFSYGSGAVSEFFTGYLEENYQEYLFAQSHQE
                     MLDSRTRITVDEYETIFSETLPEHGECAEYTSDVPFSITKIENDIRYYKI"
BASE COUNT         6329 a         3472 c         4060 g         5960 t
ORIGIN      
        1 aaaaaatgaa aaatcggtta attgtcgcat gtggaagtgg agtagcaacg agtcagacaa
       61 tcgctagtaa gatccaaagt atgttagaag atgacggcat cgcatttcct gtagaagcag
      121 ttgattacaa atcgattcaa aatgagcttc taactgctgg aatctatgtg tatgtcgcac
      181 aacctgatga ggaagtactt gaacaagcaa aagatttggg tattgaagta tttccaggaa
      241 ttccattttt aactggaatg ggtgtagaac ctatttatga cagtatcaaa gagttagtac
      301 agtaaaggag gattgttgga taaaaaagtt ggttcatagt tttatttact atatttagaa
      361 agaaagaagg tttatttatg gaagttttcc aaaccattat caattatgtt ttgaacttag
      421 gatcagccat ttttgttccc ctgattattt tgatacttgg attgttggca gggatgaaat
      481 taaaaaaagc attcatgtct gctttgactt tagggattgc ttttagcgga atgtctatgg
      541 tcattggcta tatgtctaac gcagtaagtc cagcatcaga agcattggct aaaaatacgg
      601 gaatcagttt gcctgcactt gatcttgggt ggacaggtgc agcaagcatc acatggtcgt
      661 ggagttatgc ttttatattt tttgcagtaa cgattggggt caattttgta ctgttacttt
      721 ttaattggac gaagacgtta aatgttgata tgtggaatgt ttgggggaaa gctttaactg
      781 cttatttagt ttattatgtt tccggtagtt tggctgcagg ctttcttact gcaatggtcc
      841 aagttatatt ggaattaaag cttggtgata tgtttcaaaa acatatacaa gatctcacag
      901 ggattccatt agtaacagtg actcatttta tgacatctgc cgctgtcttg ttgctgcctt
      961 tcaatatgat catggataaa attcctgctt taaataaacg agcggatacg aatgcattga
     1021 agaagaaaat tgggatcttc tctgaaaact ctgtaatggg attcattatt ggattggggc
     1081 ttggctttgc tgctgcgtat ggcgtttcag gttctcttaa tctagcgatc caaacagcta
     1141 cagcattaag tttgtttcct atgatctcta aaatgttcat gcaatctttg tctcctctag
     1201 cagacgctat gtcagaattt atgaaaaagc ggtttaagga tcgtgaagta tatatcggat
     1261 tggactggcc gatattagct ggtcgttcag aaatttgggt tactgctatt ttacttgttc
     1321 ctgtgtttat tggttatgcc attattttgc caggaaacca agtattgcca ttagcaggga
     1381 ttatcaacta ttcgattgcg gtcggcggat tacttttaac aggaggaaac ttggctcgta
     1441 tgctgatctt gggaatcatc actatgccta tttatttgta cggggccact taccttgcgc
     1501 caatactcag tgatttagca gaaaaaagcg gagcagtcga gggaatcaag aaaggacagt
     1561 tgatcacatg gtcatccata gaagggccgg agttcagggt tttatttgcg gaggcattta
     1621 ataggaatat tctcgctatt gtaggatgcg tgatttttct aggactgttt gttctacttt
     1681 ataaatatat gataaaagcg aaagtaccga gtgaacgtta tgctgaacta caaaaatagc
     1741 attgaaatgg tgggaaagta atgaaaagag aagagacaga taaaataaaa tggacagtcg
     1801 ctctttgtgg tacacttctg ctatttctat atggactctt tactcagaat atcatcatca
     1861 atctattggt catttttttc gcactagtga tctataaata tggaaatcac gttttattca
     1921 gagagtacga tgaaaaaaga aaacggaaaa tagaagaatc aatgaaaata aaagaagcta
     1981 ccaaagaaat attgagagaa aaaagtttta taaaaagata atggaggggt cattatgtca
     2041 gatggacgta tgttatttga gagagaacga caggacatgg cacgtatcgt aagagtgatt
     2101 ttcgaaagga aaaacacaaa tgtcgcagga gggaattttt cctttaaaac cactgatgat
     2161 caagggaaag actacatcat catgacgccg accatgatgt cagaagccta tctaggagag
     2221 atttctcctg cacaggttct agtagtagaa ccaagaacgc gtagaatcgt tgacggagtc
     2281 ggtcatttaa ctcgtgaaat caatatgcat gaagcgatgt atgagactaa tccagctatc
     2341 aaagctgtcc tacatgctca tgcgcctaat gcaatgttct gggcgactac tgggttgcct
     2401 atgcctaatt tgacagaagc cacagctaaa gtaaaagaga ttcctgtcat ggactttgag
     2461 cctaattgct ctgaagaact tgctgaattg acaagcgact atgtaaaaaa acagcatctc
     2521 gaattaccgc atatgttgct gctcaactct catggcgtat tgatcaacac aggtggagaa
     2581 tcaggaatcg aagctatcca taaagcactg caaatccttg atacagttga atggaatgca
     2641 gaaattgcct acaagcagac gatttttcaa gcattaggga ttactgacgg tttttattca
     2701 aaaggtgaga aaatcggggg attggaagag ttgaaacata aaacggctat ttataataaa
     2761 tctgttattg cagctggtgg agactaacct atctgacaca aaaaactacg actttcacaa
     2821 aatctgatcc agatcgatat ctgcttcagg ttgtattatg taagtcgtag ttcttttctt
     2881 ttatttagtt aagaggtggt ctgtccattc cctcgcttgc ttgatttcgt ccatcgtcaa
     2941 ctgatgacct gttacagtta gctgaatagt cacatttgct tggcgtgttt cgaattgatc
     3001 ggccaattct ttaaaggaag caggggaaac gattggatcg ttatcaccat aagaaatcca
     3061 gacggtttta tcctctaatg tggaacggct ttgatgaaca cctagagaca tcggatggaa
     3121 aaacaaccct ttatttagct cggtctcacg ctccaataat aaatgagcag cgatgtttgc
     3181 gccatttgaa tagccgacaa gcagccagtc acttaacggt atcagttctt tttcgctgat
     3241 ttctttgatt ttttcatgga gtttatctgt ttctttttcc aaactgtcca ggtcgaattg
     3301 attcaatcca ttacgcttga aaaaacgatt catcccatct tcttggaccg tgccacgaaa
     3361 agaaagcaat gtactgtttg gtgcaagaaa gtgagcgata tccagtaaag aatgttcatc
     3421 gccacctgtg ccatgaagta agatcaaatg cttgccactt gttccttttt caaaaatgta
     3481 ggtcattttg ttccctcttt tctgtttcta tagatgcaaa tgaagtagca tgaagagatt
     3541 attcacgttt tttatttgca tcacttgtat cgaaaggacg gacataagct tcaatggctt
     3601 cacgacgttc ttctaagaat ggaggaagag atagtttttc tcctgctgat tctgctggtt
     3661 catcttcaag gaatccagga ccatcagttg ctaattcaaa cagtacgtga tgagcggcta
     3721 ggaagtactc agattggaaa tagaaacgat cgacgaaacc agaatttggc aattcaatct
     3781 tattgattac ttgaatccag tattccaatg cttctttgtc tgctacacgc aatgctaagt
     3841 gatggatatt tccaaatcct tcgattgctt ttggcaagtc tgtccgatgt tcaataatga
     3901 tgcttgcacc atttccccct tcaccgattt caaataagta gaaatctcct gaatggtctg
     3961 tttctttgaa tcctaagatt ttttcaagaa tcaagcggat atgctctaat tgactgacac
     4021 ggacaaagac aggtccaagt cctgtgatcg caaactctgt cggtacattg ctgtttttcc
     4081 aaggaatgcc agctgctaca ccttgattgt tttcgtcaga gatcaattga taatactgat
     4141 tatcgaaatc ttcaaattcg atatattttt tgccgaaacg ttcattgatt gctgaatgag
     4201 taacgtcata tttttcaaaa cgttccaacc aaaagtctaa tgctgcgtca cttggcacac
     4261 ggaaagaagc acgagagatc gtatctgttc ctttttttcc ttttggctga tttggaaaat
     4321 caaagaaagt catatctgtt cctggcgcac cagtatcgtc tgtaaagtaa agatggtaag
     4381 tttcgatgtc atcttggtta actgtttttt tgattaagcg cattcctaaa atatctgtaa
     4441 aaaagcggta gatttttgta gcgctagatg tcattgctgt gacgtggtga attcctctga
     4501 tttgtgtatc catgataaat ttcctcctgt taacttacaa aaagtaagta gttgatttag
     4561 acatagtata gagggtgcat ctctattttg caattaattt gtcttctaaa gtatcaggaa
     4621 ggaatcaaga atggaaaaga ggagggacgg atcaataaaa taaaaagact tacgactagc
     4681 tgtatcgatt tcaaaaaaac tagctttaaa aagctaactt tagaatcatt acgggtggtc
     4741 ataagtcttt ttatgctgag ttattttttc atttttgagc taatgcttag gatagaatag
     4801 aatgttgatt tcacgatctc ttcttgttac ttgaaactgc ttaccactgt cacgatctct
     4861 tggtaaacgg ctttttcaca acctctttgg ttaagttgaa cgcacatcca gcacagaata
     4921 gttccgaaca tcaaatcggt ttcggctgga agaatattca atcggctgtc catctttgag
     4981 atagacgact tgctgaactt ccagtacagg atcgttttct gcacagttca aatattgctg
     5041 atcatattca tcacttttat ctgcagaaat agcacgataa gcccctgcaa aatggacatt
     5101 caattcttga tgaagatgat catagataga ggctaacaaa tgttcttttt ttaaacccgg
     5161 cacaagatgg actggcataa aggtatgctc taaaacatat ggctcgccat ctaagatcct
     5221 taaccggtga atattgtaga caggttgctc ggcatcgatc atcaatcgtt cttggatata
     5281 ttgattaggg aattcaacat caaaatgaat gatttgactc ttcaaagaac gatgtctgcg
     5341 tttcatttgg agactcaaac cttcatactc gttagcgggc gatgtgtctt tttgcataaa
     5401 tggatgattc aagattttcg ttcctgatcc tcgctgcgaa tagacaagtc cttccattgc
     5461 taagatattg atggcttttt tgattgtcat ccggctcgca ccaaattctt ttaccaagtc
     5521 gacttgatta ggcaaaaaac tatcgagagg atactctttt gaacggatac gttctcttaa
     5581 aatatttgcg atttcttcgt attttggcac gttattcacc tcttctagag tataacgaaa
     5641 gattatgctt ccgacaactc gaagtagcat ctggaacatt gtttattcga aaaaagaggt
     5701 catgacaagt ttttgtcaca accccttaat gatctattaa gcaatatcta aatctggttg
     5761 tttatctagt actttcagga atgggtacca gataaaggca acgatgacaa agtcgatgac
     5821 ttgcagcata ccaccaagcc atgaatttgt tgctaagaca ccgcttgcaa tgattggcac
     5881 agtccatgga acagagacgc cagttggtgc agggacaagt ccagcagcca ttaccaaata
     5941 attgaacgtc gttacgatta gaggtgccac aatccaagga ataaggattg ttgcattcaa
     6001 tacgatcggt agaccgaaga tcgctggttc atttacattg aaaataccag gtgctaaagc
     6061 taatcgtcca acatcacgat actgtttctt tttaagaaca aaggccatca ataaaacgac
     6121 agctaaagtc attccagagc caccaatacc aacagtgaag gtttccatga atggttttgt
     6181 gatgatgtgt ggtaattctt ttccatgttg ataagcttct aagttatcaa gcatcagtgt
     6241 attccaaatc gggtccatta cagagttgac gatgatttgt ccgtgaagtc cgaagaacca
     6301 taagaattgg acaaagaaca aagcaatcaa tgttgcaggt aaactacttc ccaaaccagt
     6361 taatggtttt tggatcactt cataaacgac gtcatgtaag ttagcattga aaataccagt
     6421 catcacagca ttgattacta agaaaatagt tagtgtcact acagcaggga tcaaagcggc
     6481 aaatgatttt gttactgcag gcggaactcc atcaggcatt ttgatctgcc agccgcgttt
     6541 cgtgattctg cagtagattt ctccagccaa gaaagcagcg atcattccaa tgaacattcc
     6601 ttttgcgcca agacgatcaa gtgaaagcac accagtcaca tcttcaccgc cagcggtttt
     6661 cattgcaaaa ggcgtaagaa tcaagaagct ggcaaaagac acggcaccac cgaaaatacc
     6721 ttcaacgtca taagattttg ataaataata gccgatacca aaagtaacaa aaaccgacat
     6781 gatgctcatt gttgcatttt gcccattacc gaataaattc gataaagttc cctttgttgc
     6841 gtcactgaaa aaaggcaagt tattgataac gacaacgatc gaaccgaaca tcgttaaagg
     6901 gaaggaaagc atgaaagcat cccgcagcac tgtaaggtaa cgattttgcc ctaataaatt
     6961 agctaagggc atgattttct cagaaagctt gtccataaaa ccgttcattt ttattcacct
     7021 caaatttatt tgcaatcgat tacatacaaa gtataacgcc aaaaatttta aatgtctagt
     7081 cattttcata aaaagtattg atttattttt tagggttcgt tatgattacg gtatcaagaa
     7141 aaaagagagg aagtaaaaaa atgaaaacct acacattccc agaaaatttt tggtggggtt
     7201 ctgctgctag cggaccgcaa acagaaggaa gagtaagtga tgatggtaaa ggagaaaata
     7261 tttgggatca ttggtatgaa ttagagcctg aaaaattctt cgatcaagta ggacctgaaa
     7321 aaacttcgca agtttacaca aaatacaaag aagatgttca gttaatgaaa gaaactggcc
     7381 ataacacatt tcgtacatct atccaatgga gtcatttgat tccagaaggg acaggtgaaa
     7441 tcaatcaaaa agctgtttct ttttatcgag attattttac tgaattgctg gataatggta
     7501 ttgaaccttt tgtgaacctt taccatttcg atatgccact accactgcaa gaaaaaggcg
     7561 gatggttatc aagagaaacg gtcgacgctt atgtttctta tgcagcagcg tgctttgaac
     7621 tgtttggaga tctggtcaaa aaatggttta ctcataatga accaatcgta cctgtagaag
     7681 gaggctactt gtatcaattc cactatccaa atcatatcaa tatgaaagaa gcggtccaag
     7741 taggctatca tgagaatcta gcaagcgcaa aggtaatcaa agcctaccat gaaggcggac
     7801 atgatggtga gatcggaatc atcttgaatc tgacaccaag ctatccgaga gatgaaacga
     7861 acccagaaga tgtaaaagcg gcgaagatcg ctgacgcatt cttcaaccga tcattccttg
     7921 accctgcagt gaaaggagag tttccacaag agttgatcga aattgtcaaa gaattggcta
     7981 taatgccaga gatccatgaa ggagacctag aactcatccg tgaaaacacg attgacctgc
     8041 taggaatcaa ttactatcag cctcgtcgaa tcaaagcaaa agaaactcca atcaatcacg
     8101 aacatagccc catgccagat gattattttg ataattacga catgcccaat aaaaaaatga
     8161 atccatatcg tggatgggaa atttacgaaa aaggaatcta tgatatttta acgaatacaa
     8221 gagaaaacta tggtaatatc aaatgctata tttctgaaaa tgggatgggg gtcgaaggag
     8281 aagaacgatt tgttaacagc gaaggaatta ttgaagatga ttaccggatc gagtttgtta
     8341 cggatcattt gaaatacgtc catcaagcaa tccaagaagg aaccaattgt gtgggttacc
     8401 acatgtggac atgtatggac aactggtctt ggacaaatgc ttataagaat cgctatggct
     8461 ttatcgctgt tgatttggca caagacggta aacggacgat caaaaaatca ggtcgctggt
     8521 ttaaaacggt ctctgaaaaa aacggatttg atgcctgata ttttttgata aaacgttcac
     8581 aaaatgtttg ttctaacact gtaatcgtgc tacactaaca gtgaaggagg cgtttacaat
     8641 ggtagatact tatcgtaata ttttagtagc aattgatggt tctgaaaagg cagaaaaagc
     8701 atttcttgag gcaatcacga ttgccaaacg aaatcaggca acgttgcaca tcctgtatgt
     8761 caatgaagta acaggcaatt actttggtga ttttgcattt gttacaacaa gccttcagga
     8821 agaattggat gaggttgcgg aaaaccagat gaaagaacat cgaaacttgg caattgaaaa
     8881 aggcctgaca gatatcgaga catatgttct ttacggttat ccaaagacac tgattgctaa
     8941 tttccatgaa tcgaaagaac caatcgatct gattgtcatg ggggcgacag gtctgaatgc
     9001 ggtagaacgt gcactagtag gttccactac ttcctatgta gtcaaccacg catcatgtaa
     9061 cgtcatggtt gtaaaataaa taaattaagg gaacagaaag caaaaaagca taacgcttct
     9121 tttgctttct gttcctttta tttttttgta aaaaattact ttacctgatg aagtaatcat
     9181 gctattctaa ttacaacgac agatggagta atatggagga aagaaaatga tttcaagtga
     9241 tggaatacgc ggatacaatg atgcgattat tttatcgatc cttaacaaaa acgattctta
     9301 tggatatgaa atttctaaaa cgattcgtga gaggacaaat gattcctatg tgatcaaaga
     9361 gacaacactt tactccgttt ttgcccgttt agaaaagaat caactgattg tttcgtatcc
     9421 tggagaagtg actcatggaa aaaagcggac atattataaa atcactgaaa tagggcgtaa
     9481 tcttttagaa gaaaaaaagc aagagtggct gacgacgaaa gacgtagtcg atcaattttt
     9541 ggagggaaat taaatggata caatccgtaa ttatttagac agtcttttta tcggagtacc
     9601 tcaaagtacc gagatagata agctgaaaac tgatttgttg gcaaatatgg aagaccatta
     9661 tcatgaattg atgggagaag gaaaaaatga acaggaggcc atcggtacag taatttcgac
     9721 atttggttca atcgatgaat tattagaaga actagatgtc gaaaaaaaac atcaagctga
     9781 cgagacggaa acaaacacag cttctattta tttaagcgaa gcagaaaact actggaaaga
     9841 atacagagcg gcaagtctcc aagtggcatc aggtgttcta ttcattagct tatcttttgc
     9901 aagttttctt ttcttttgtt cagcaggata tgtgttcatg ggtatttctt gtctgatttt
     9961 cggtatagca ttagctgtcg gtttttttat tgcttctggt atgaaaatca cacgtttaaa
    10021 tcactttttg caccatcgga aaatacctga aaaagtatta gcagaagcaa aagaaaaaga
    10081 agaagagtat cagcgaagtt ttggcttctc actgattgca ggaattggat tatgtatttt
    10141 ttcacttttc ccacttttgg cttctttaat gtggtatatg gatggtagta tcggtgcctc
    10201 tatatttttt gttacggtag gaacgggtgt cttcttgatc atttatggca gtctagtccg
    10261 tcattcttat cgtcaattca ctcaaagcgc ttattactgg tgaatgtatt cagatttgta
    10321 aaaatattct atttatccgg cgtagaaaaa aagactgtga cacgcttttg tcacagtctt
    10381 ttttatatat aataatttaa aaattcatca tctaaatccg tagaattttc tagattgctt
    10441 acccatgtaa aatcgttata gacagatttc agttgacttc tgcgccattg tacagggata
    10501 aaaagacgca tgctatcttc attgaatccg actaataatt tctttccgtc agtaatgatg
    10561 ggacgttgta acaaagtaaa atgcgagacg atcagatcga caagctctga ggtcgtacat
    10621 tggtcgattt ggtctttcaa atttttataa tcaatcgagc gtttagctaa tagatcgtca
    10681 aatccgtttt ctgttaatga caataaataa aatatctctt tccttgttaa aggctgttta
    10741 ggtgtgaaaa ttcgttctct gtaaggcatt tgatggtctt tcagccagtt tgccatcaga
    10801 tggtaactat gtgtgcgaga agaatagatc gtaagcataa aaaaactcct taaatagtcc
    10861 attaatttgt tagatctgtt gagataaata ataggtaagg agctgtgcat aagcattgat
    10921 caaaacaacc caaacatcat tagaaataac tttctaaagt cttaaacttg cgacgagaaa
    10981 caactagact aatagaaaaa atgtcttagc catttggctt cctgtttgtt tttttgctta
    11041 tgctgcagct cctcgggaaa gggttctaac aatttgaaga aggttgatga ttgatctagt
    11101 ttaaattgtg tgaaatttga aatctggtgt ttttactaga tgaatcacct aacaactatg
    11161 attataactt atagaacttc tgttttgcat tcggttttgt tatagttgtt ataaagtttt
    11221 attttattac agaacatttt taattaataa agaacagtta gaagttaaaa ttgctgattc
    11281 acatagagtt tatgacttct tggaaattct ttttcaatca gttttttcat ctgtttatga
    11341 aattcttttt tttcagtaaa cataaaggtt tgggtatcgt aaccttcttc atgtaggaaa
    11401 tagattattc ctttttctgt gttttgcgta taagctttta tactcctgtc ttttttgaaa
    11461 gaacagatcg tatagccatc tatttggtct ggtagtcgtt tgattttctt caaaatgact
    11521 tttttatcct caagtttcat ttttttcacc tgactttatt tttttcatat ggaaatcata
    11581 gcgcctttgg ttatatgaaa acaagaagga tactgaaata tatataaatc aaatagtggt
    11641 tatttattat tagaatagcg aatacaaata aacgaccact attttctgat ttattgcaaa
    11701 tgttttttca aataaatttg attaccaaga aaaggtagtg gtatatttac tgagggatac
    11761 tttgaaaaac atatgataga aacagtaaca gtattatgaa aaatcttttt ataaaaatgg
    11821 aggaatcgat ttgatggaag atcagtatgt acaagcaatg acgatcgcag gatcagatag
    11881 cgatggaagc gcagggatgc aagcagatat gcatacattc tttacaagaa atgtttacgg
    11941 tgtttctgtc atgaccgcat gtgttgcagg taattcttac ggaatcggag caagtgtgac
    12001 attgccaaca gattttatcg acaaagaatt tgaactgatt gcgaaggact ttcaaatacg
    12061 tgcagctaaa acaggtatgc tagcagattc aaagttgatt gagacagttg taaaaaatta
    12121 ccaaaaatat gattttggac cattagtcgt ggatcccgta atcgtaacga aacatggaaa
    12181 tcttttacta gaagaatctg ctttgcaaag cttgaaagaa aagcttgtcc ctttggccga
    12241 agtactgaca cctaattttt atgaagcaga aaagctgact ggacggacat tagcagctga
    12301 agaagatttt gtgcaagcag caaaagaact gcaagatatg ggtgcaaaaa acgttatgat
    12361 caaaggacat catgctaaaa atgcgcagca agaaacagtc aatgactacg tattgttcga
    12421 atcaggaaaa tcattttggt taaatgcacc attttatcct accgaccgga tcaacggaac
    12481 tggcgattcg ctatctgcat gtattgcagc tgagttagcg aaaggaaaaa cagttgaaca
    12541 agcgatccga ttggcaaaag actatgtaaa ccgtgcaatt cgtgaagaaa taaatgtagg
    12601 gcacaaatat ggccccatca atcattgggc agctggtgaa ttagagtaaa agaaagaagt
    12661 tgtgaaaaag ttgccctgca tcaagtaata agaacagcca agtaaaaaca gcttcttatg
    12721 tttttaactt ggttggactt attaccgcag atgcaagctt ttgaacaccg tttaagaaag
    12781 aggtcgtgag cctgccgtcc agctccgagt cacaaggaac aagttcaaaa aacagcttct
    12841 catgtttgtt gaatattgtg acttgtgacc gaggagttgg caggagaaca ccgtttgttc
    12901 ggaagaaggg actgtgacaa gatttttgtc acagtccctt taatcttagt atatttttct
    12961 ctacttgaat aaaaaaatta taggattgga gaaaataatc gagaaatttg ttgcttgaat
    13021 aatagccagt tagacatttc cttgactgtt tcagttgtca tggatgaaca atgtgtctca
    13081 tcttctttga actgagtagc aagttcttct aaaaactcag gatcgtaaat gacagcactt
    13141 gtttcaaaat tcaatcggta gctacgaata tcttgatttg ctgaacctac catgcagatt
    13201 tcatcatcca ttatcaatgt ttttgcatgg aggaagccgt tctcatagac aaggatttgt
    13261 acattttcct tgatcagctg ccgagcataa tattgtgttg ctcgataaat aaaaggatga
    13321 tccggtttgt tgggaatcat gattttcaca tctacaccag aggcagttgc gatttttaaa
    13381 gcagcaatga cactttcatc aggaacaagg taaggtgtct gtatccaaac acgtttttta
    13441 gcagaagtaa tcaatttgat aaatgaaagc ttgatttgtt ccctttcgtt attcggtccg
    13501 cttgaaacaa gctggatgga tgtatcagca agcttagatt catctcgatc tgctttttta
    13561 aagaaataat tcaaatgata gccgaccttt ttttcttcag gtaccgagac gttccagtct
    13621 gttaagaagc gaagctggag caaagaagaa gcggcaccga aaatccgtat atgcgtatcg
    13681 cgccaatagc cgaatttttt tgttgtattt acatattgat tggcaatatt gaaaccaccg
    13741 gtataactaa tctttccatc gatcacaacg atttttcggt gatcatggta attcaatcgg
    13801 aaacgaagta atgccctttg tgaggtaaca aatgtatgga catgtccacc atttttgatt
    13861 agacgattga aatcttttgc ttttgtgcca tgagagccaa atgcatcata taatattcga
    13921 acttccacgc cttctgcggc tttttcttct aaaacatgta agacttgctg gccgatatta
    13981 tctgtcacaa atgcgtaata ttcgatatgg atcgaatgtt tggctttttt tagatcttga
    14041 agcagtgaat ccaatttctc ttgtccgtct gtgagaagag tgacagaatt cattcttgtc
    14101 agcggcatac gatttaatga agagaagaag tcaacaaatt gctgtttgtc tttatcgccc
    14161 atgtcaggat cgtaatgttc aatggtatct cctctaaaag actgaaagtt ttctaattct
    14221 ttcaagtcac tttgtcggag atagaatttt ttcttatccg ttaatccacg tccgaaaaat
    14281 aaatataata caaagcccac cccaggaagg gcaaacaata ctaagagcca tgcccaaatg
    14341 gctgctacat ctcggggttt gatcaaaata gccaccaaag caataagtgc atttaataga
    14401 taaagggcgg ttataatact agataccaca aaaatgttcc tccttacata caataacatt
    14461 agtatagcat gatagaagaa agaagcctac caaggtgaat atacttttca ctcctttcat
    14521 gaaaatgtag gtaaatcggg ctaaagtagt tgctttttgt aggttaataa ggtaggatat
    14581 tagtacagtt gaatatctcc ttaggggagt aaccggcaac gtttgttgcg ataatatcaa
    14641 cagtaagtta accaaatggt tttctggtat tgtatgaaat ggtgagacct aagacaaatg
    14701 cgtatttgtc ttaggtctct tttttttcag acaggactag ggccaagatc aaaaaatggg
    14761 ggatgaaatt tggaaaacaa ggaacaagca caacaagcgg tctataaaga aacgatcgac
    14821 tcgttgatgc agcaaacgca gagctcaacc gatggattga gcaccacgca agcgaaggaa
    14881 aaattagaaa gagatggctt gaaccagcta aaagaaaagc cgaagaagcc aacttggaaa
    14941 ttgttcttag aaactttcaa agatgcaatg gttatcgtat tgctgattgt cgcactgatt
    15001 cagatggtca tgggtgacta catcgaattc ttagttattt ttgcagtatt gatgttgaat
    15061 tctataatca gtgttgtcca aacaaaaaaa gcagaaggtt ctttagaagc gttgaaaaac
    15121 ctttcagcgc ccgatgctcg agtgattcgt gatggtaagg aaaaaacgat tccggcaaac
    15181 gaacttgtgg taggagatat cgtgttgtta gacgcaggcg attatgtacc tgctgatgga
    15241 cgtttactag atgcgggatc attaaaagta gatgaaggta tgctgacagg agagtcgact
    15301 tctgcagaaa aagaagtaac ggatatcaca agtacggtac caatcggaga tcgtacaaat
    15361 atggtattca gtggaacgat cgtaacttac ggacgcggta aattcttagt gacttcaact
    15421 gggaataata cggagatcgg acaagttgct ggccttttgg aatcaacaac tgagcaagtc
    15481 actccgttac aacgaggtct ggataaattc agtaaaaaac taagtttggc tattttggca
    15541 ttgtctttat tgattttagg tatccaactg tttcgtattt atcttggaga aggtacagga
    15601 gatatgactg cagatatcgt cagcgcagtg atgtttgccg tagccgtagc cgtagctgct
    15661 attccagaag cattacagtc tatcgtaaca atcgtgttat ctttgggaac gaataaaatg
    15721 gcaaaacgtc atgccattat tcggaagctt ccagcagttg aaacacttgg ttcgacaagt
    15781 atcatctgta cagataagac tggtacattg acacaaaaca aaatgacagt tgttgactac
    15841 tttttaggta atggcgatac tggggatttc acaaatgacc cttcaaaatg gacagctgac
    15901 gaacgtcgct tgatcgaaat cagtgtttta gcgaatgatg catcgatcag tgaagacggt
    15961 actaaattgg gcgaccctac ggaagtagcg tttatcgact ttagtgaaaa attaaaccag
    16021 ccttatcagg aaatccgaaa caactaccca agacaagcag aacttccttt tgactcagat
    16081 cgaaaattaa tgtctactgg acacacaatt gataacaaaa attacttgtt tgttaaaggc
    16141 ggaccagata tcgtcttcca acgcagtaaa aaagtattgc taaatggtga agtcgttccg
    16201 atgacggatg aattgatgaa aaaattccaa gagcaaaatg aagctttttc aacacgcgca
    16261 ttacgtgtac tagcttttgc ttaccgtcca cttgataatt ttgatttatc atttgaagat
    16321 gaagacgatt tgatccttgt tggtttgctt gctatgatcg atcctccacg tgctgaagtg
    16381 actcaggcgg taacagaagc aagaagtgca ggaatcaaaa caatcatgat tactggtgac
    16441 cacaaaacaa cagctgttgc tatcgcaaaa caaatcggga tttttgaaga aggaaacctg
    16501 gctttgaccg ggacggaact agatgaattg acagaagatc aactaaacga agttttagaa
    16561 aaagtcactg tttatgcacg tgtttcacct gagaataaaa ttcgtatcgt tcgtgcttgg
    16621 caaaataaag gtcatatctc agctatgaca ggtgatggtg taaatgacgc accagcattg
    16681 aaacaagcag acatcggaat tgctatggga acgggtacag atgtggcaaa agatgcagca
    16741 gcgatggtat tgacggatga taactttgct tctattgtca gcgcagtaga agtaggacgt
    16801 aatgtgtttg ataatatcaa gaaagctatt tcttatctat ttgcaggaaa tttaggtgcg
    16861 atcattgcga tcatctttgc cttagttgct gactggtcca atccatttac agcattacaa
    16921 ctgttgttca tcaatttagt caatgattca ctgccagcaa ttgcattagg tacagaaaaa
    16981 gctgaacctg ctacgatgaa ccgcccgcca agagatccaa attcaggaat ctttacggac
    17041 aaaacattga tttctgtaac gtatcgtggt gtgctgattg ggattgctgt tatctttgct
    17101 caatggcttg gcatgcagca ttcacctgaa ttcggtgtag ccatgtcatt ttcaacattg
    17161 atttggtgtc ggacattgca aacattccca gcacgttcaa acacacaaac agctatccaa
    17221 gcagggttct tctctaacag aagtgtctta ctagctgtag ttgtatgtag cgcgctttac
    17281 tgcttaacaa tgatcccagg actgaaagaa gtcttctcta tcccatcagc atttggattg
    17341 acagaagtct ttacatgtat gggaatcgca cttgtgacaa ttgttttgat ggaaatcact
    17401 aaattgatca ttgtacgagt acaaggaaat aaataaaacg aatcaacgag gttgtgagcc
    17461 tgccgtttaa ttctgagtca caaggtgcaa tttcaacaaa cagcttctca tgtttgttga
    17521 atattgtgac ttgtgaccga agagttggca ggagaacacc gtttaaggaa gaagttgtaa
    17581 aatcagcatt ttgagctaat actgagggtc atctgattgg acactgttta ttcggaaaga
    17641 gggactttga cacgactttg gtcacagtcc ctttttttat ttatgataga cggaaagcca
    17701 atcattttat tattcatcaa aagagataga atagataaaa gaggagaatg atttattccg
    17761 aaaagtgaaa acatataaca aaaaattaga ggaaatcaaa tgaatgaaaa agaatgggat
    17821 gaatttgctg aagaatatta tgaaatccag caagaatcgc aagtaactat tgctgaagat
    17881 gtaaaaaatt atctgaaaac ggagaaagtc ctgcctgctg aaacagcagc agacgttgct
    17941 ggaggatctg gcagatttct ttccttagca aaagaaatta atcgctatga tttgatcgat
    18001 ttttcttcag gaatgctaga aaatgcaaaa aaagaagcag aaaaaaatga gttgccaaat
    18061 gtttccttta tcaaacagtc gttagaagaa tttatccaat caaatgagac atatgaaatg
    18121 gtttttacag cagcaaatcc agcattggta catcttattc aattagaaaa attgatgaaa
    18181 aagtcaagca aatggtgcgt gataatcaga gtagtggaat caatcgatga tgttttttat
    18241 cctatcgaaa agcaactagg aatgatagaa gaagatccta atacatcgcc agttgtaatg
    18301 gatcaatttg aaacatactt aattgatgaa ggattcaaag tgaagaaaaa agattttact
    18361 tattttacac aggaatcaat tactgaagaa ttgatgaagc tatactatga cgcgtacgca
    18421 aaagatcctg ttgtgaccag gtacattcgc aaactattca gtgaaaaacg agaaatcctt
    18481 agtacgacta agctgaccta tagactgctc ctttggtccg cagataaaaa aagaaccttt
    18541 cacgactcgt gaagggttct ttttatgatt ttattatatt ttgtagtaac gaatatcatt
    18601 ttcgatcttt gtaatcgaaa atggtacatc tgaagtatat tctgcacatt ctccgtgttc
    18661 aggaagcgtc tcactgaaaa tcgtttcata ttcgtcaaca gtgatccgtg ttcgagaatc
    18721 tagcatctct tgatgagatt gtgcgaagag atattcttga tagttttctt ccagataacc
    18781 tgtaaaaaat tcacttactg caccagaacc gtagctgaat agaccgatgc gatctcctgg
    18841 ctgtagtgat ttcgaatttt ccagtagaga agttagcccc aggtataatg aaccagtgta
    18901 aagattaccg attcgtcggc tgtaacggat gctttcttca tagcgagcca taagacgttc
    18961 ctgattttct tcgtctgttt ggtctaatac gctttgcaat gccttttttc ccatcttagt
    19021 atacggaata tggaaagcaa tcgcctgata atcttcgagt cctcgacccg acaattcttt
    19081 atgtcgattc caaacttttt ggaatgattc gatatacgta gaattagata aaggaccatc
    19141 aacaacagga aattcgctat aatctggacg ccagaaatca tagatatctt ctgtcagaaa
    19201 tacgctgtcg tcttcaatcg ataaaatacg cgggttttga gtgatcatca tcgcaacagc
    19261 accgacacct tgcgtcactt caccaccgct tgccaagccg taacgagcaa tatcacttgc
    19321 tatgactagt acttttcgtt ctggatgatt tttgacatat tctttcgcca tgtgcaaagc
    19381 tgcggtgcca ccgtagcaag cctcttttac ttcaaaagaa cgagcaaaag attggatttt
    19441 cagtaaatgg tggataatta ccgcactcgc ttttgaatga tcaatcccgg attctgttcc
    19501 gacgatgacc atgtcgatta gctcacggtc tttttctgtt acgattttgc tggcagcatt
    19561 tgctcctagt gtaatgatgt cttcatttgc acgattgact gccatttggt cttgaccaat
    19621 ccctatatgg tattttgcag gatcatcccc acggctttct gccaactctg tcatatctaa
    19681 atataaatta ggaataaaaa aggaaagacg atcaatccct attttcattt caacacctct
    19741 ttacttatga taaaaaactt actttactaa ggtatcataa aaagattaaa agaaatataa
    19801 aaaagagaat atttaaggat t
//
LOCUS       DARBET010000048        19475 bp    DNA     linear   BCT 07-NOV-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4027
            SAMEA114569841-rid20112183.denovo.049, whole genome shotgun
            sequence.
ACCESSION   DARBET010000048 DARBET010000000
VERSION     DARBET010000048.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA114569841
            Sequence Read Archive: ERR12203666
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 19475)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 19475)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001972618.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001972618.1
            Genome Coverage       :: 132x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/07/2023 13:18:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,041
            CDSs (total)                      :: 2,975
            Genes (coding)                    :: 2,908
            CDSs (with protein)               :: 2,908
            Genes (RNA)                       :: 66
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 67
            CDSs (without protein)            :: 67
            Pseudo Genes (ambiguous residues) :: 0 of 67
            Pseudo Genes (frameshifted)       :: 26 of 67
            Pseudo Genes (incomplete)         :: 28 of 67
            Pseudo Genes (internal stop)      :: 20 of 67
            Pseudo Genes (multiple problems)  :: 6 of 67
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19475
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA114569841-rid20112183.denovo.049"
                     /isolate="VRE4027"
                     /db_xref="taxon:1352"
                     /country="Denmark"
     gene            complement(21..995)
                     /locus_tag="SCJ86_001870"
     CDS             complement(21..995)
                     /locus_tag="SCJ86_001870"
                     /EC_number="2.7.6.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375717.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribose-phosphate diphosphokinase"
                     /protein_id="HEG1169408.1"
                     /translation="MSNKYQDDTLKIFSLNGNRPLAEKIAKVFGTELGKSVVKQFSDG
                     EISINIEESIRGDHVYIVQSTNAPVNDYYMELLIMIDAMKRASAKTINVVLPYYGYAR
                     QDRTAKPHEPITAKLIANLIEEAGATRVLTLDLHTVQVQGFFDIPVDNLFTMPLFAHY
                     YRQLGLTGDDIVVVSPKNSGVQRARSLSEYLNSTLAIVDHADDASTEGGYVIGDVQGK
                     TCIMVDDILNTGATLARAANVLKENGAKEVYACASHGLLSDHAKSILDNAPIKDICIT
                     DSVFTEENRHPENLTIVTCSELMGEAVKRIHENTPMSPLFRLEEKEFE"
     gene            complement(1075..1950)
                     /locus_tag="SCJ86_001871"
     CDS             complement(1075..1950)
                     /locus_tag="SCJ86_001871"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327421.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrolipoamide dehydrogenase"
                     /protein_id="HEG1169409.1"
                     /translation="MKKRRRSQLNQVVDKPLYFKVDKKIKKLASTQQLQSRKSKLLFL
                     ALVFEDQSYVIIDQSGHPVEYSPAKYTYQEGLSCKKWKLINEEPIELSRWINRKEDIP
                     VLIEEKRSGKELANCWVGLPEERFLRYKKWATPSGYLCGTYAAAVLLAYYQDYRKGWM
                     LPNEIRKKNTADSLVLTKALRSQIQPLGLPTIPFQVSTGISSFLKKNGNHERARATLL
                     GSWQRATKRIREGKPVMIGILKVLGSTYGNHWVTAYAYFETETGERYYKVHDNWGDYH
                     KVIPASWSNGTVSLP"
     gene            2080..3987
                     /locus_tag="SCJ86_001872"
     CDS             2080..3987
                     /locus_tag="SCJ86_001872"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291213.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M13 family peptidase"
                     /protein_id="HEG1169410.1"
                     /translation="MNKERLKQDFYEAVNEEWQKTAKIPADKPATGGFQDLVDGIDQL
                     LMGETDDLLAHPEKVSSEKMKHFLAYYQLANDYDRRNELHAQPLQPIIEKVESLQSFD
                     ELNKQFPEWLLDTLPLPFNLDVDADMKNAQTNAFFAYPPSLFLPDKTYYASDHPNGAQ
                     LIGVFFDMAVQLLELAGKEKSQAEAITEQAVQFDKLIAPHVKSAEENADYSKMYNPQS
                     FDTFIKYTDKLDLKKLVTGLIGTTPDKVIVTDPVYFDHLNDILTEAHFPLFKSWMIIK
                     TVRSLSGYLSEEFRQVSGIYSRVLSGTDEAMPQKKAAYYLASGQFDQVVGDYYGKKYF
                     GEAAKKDVEQMVKKMIAVYKKRLEENDWLGDATKEKAVIKLNNLGIQVGYPDKIPALF
                     DQFKTVPADQGGTLLSNTLVFSKLTLKDRFSKWNKPVDRSEWEMSADTVNAYYHPFRN
                     VIVFPAAILQAPFYSLEQSSSANYGGIGAVIAHEISHAFDNNGALFDEYGNLNNWWTE
                     EDLTQFQNKAQAMIEEFDGLPFADGKVNGKLTVSENIADAGGLSCALEAAKTESDLSI
                     EEFFVNWATIWRTKAKKEYQQLLLQIDVHAPAKLRANVQPKNLDEFYETFGITEQDAM
                     YLAPEKRVRIW"
     gene            complement(4528..7806)
                     /locus_tag="SCJ86_001873"
     CDS             complement(4528..7806)
                     /locus_tag="SCJ86_001873"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008265555.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1565 domain-containing protein"
                     /protein_id="HEG1169411.1"
                     /translation="MLKKRIKYSALMMNLLMLSTPVLISVVEVAANEQQSVNEENAHV
                     NIEGMSTMEEVPRTSEDMDTEEGNDPMMKESENEKTKKQSEEANEEWKGVVFGESTSA
                     SRYAIEPLEDGVRLSSTNNGGKFQSSGSDGMTYYYQAIPKDINFRMRATIEIESWTYT
                     NAQEGFALMVRDAVPKNHLFGQAFYSNSFAILGSRIEYVWDSDRQEVVNTSGSKYTMR
                     LGLGTRAITGISSEDPQAAPAPGSVSVETTPLETSAGFLEKETGSYNIFGNGHGNLFP
                     ATNSITKLDVEMKKTNTGLESYYYDPETGEEMASDIMYDWEKLYASDESVIYAGFAAA
                     RNMTIKVEGIEVAYSDPATDPPGQERPTRLIDPIYTVTSSNHSGNQDHTMSFRANADG
                     LLTIKNKATGEVLKNAKNLAITANREFDYQTKLMEGTNEFTFSFTPDKNYPPEEYVEM
                     TSYETKEILHTVDYRKPKYSTVYVTPEGSDAGNGSRQNPLSLTQALRYAYAGQTIVMA
                     PGTYSFERGLTIPKGVNGTRENPIQLIAEKNPENKRPVLDFKGTGNGMTLFSHYWGLR
                     GFDITNSAAMQKGLQISGNHNLIEEIHAYRNGNTGIQISGSSNDPFEAWPAHNLVKNC
                     TSFENADPGFEDADGFAAKLTIGEGNVFDGCIAYHNADDGWDLFAKNETGSIGKVIIK
                     NSVAYRNGWVPGIEGEGNGNGFKMGGSSLTGPHELINSLSFENLAKGIDSNSGTDIIV
                     NSSTSFNNGSYNVALYTSSAGNTDYHASGILSFRTENLEMREQIRPLNQNEDQIYHSL
                     NYYWNEREKQSENTLGHTISKDWIESLNTCSKEGQQPVTRYENGSIHVHGLMQIKPGN
                     RQTEKERVDGLPLSVGAIFDGETSPSSEYPEYAIVQEPNQTKPNEQNNPIIIQEANGV
                     AENAEGNIIELKPFTVFVSTFTPNQGTPVFEDMRYFPEKIQIVETQSSAQFSNHTAIF
                     SAPSKGEYTLEVTYRSEIFDGEKWSAHTESVTQQKAITVRETADVPPGNDKPERENPG
                     NESPNGKPNGKPSHPDRTAKESEKNGTQGLPKTGEFNNPLLALAGGILLIGVVVYVMK
                     QRKK"
     gene            complement(8077..8622)
                     /locus_tag="SCJ86_001874"
     CDS             complement(8077..8622)
                     /locus_tag="SCJ86_001874"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318487.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine hydrolase"
                     /protein_id="HEG1169412.1"
                     /translation="MNALISIDYTYDFVAEDGKLTTGTAGQVIEKKLTAHTKKFIDQK
                     DFVVFAIDAHDPKDSFHPENRLFPPHNVIGTGGRELYGSLKSLYQDYEQEENVYWIDK
                     RHYSAFSGTDLDIRLRERQITDIYLTGVCTDICVLHTAVDAYNLGYKLHIFKDAVASF
                     DPVGHEWALRHFESTLGAEIL"
     gene            complement(8634..9326)
                     /locus_tag="SCJ86_001875"
     CDS             complement(8634..9326)
                     /locus_tag="SCJ86_001875"
                     /EC_number="3.2.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736197.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5'-methylthioadenosine/adenosylhomocysteine
                     nucleosidase"
                     /protein_id="HEG1169413.1"
                     /translation="MKIGIIGAMEEEVKILRENLSEPLSWERAGALFISGSLGNHEVI
                     VVRSGIGKVLASITTSLLIQQYGVNMVINTGSAGGIGEGLQVGDIVISDKVAYFDADA
                     TGFGYKPGQLPGMPLYYEASTYLRSEMVKAAKATNLNAKEGLIVTGDTFVDSPDKIKE
                     ILTNFPEALACEMEGAAVGQTARQFNIPFLIVRAMSDTADHSATQSFDEFIEDAGKRS
                     AEMVIEFVKHLV"
     gene            complement(9345..9629)
                     /locus_tag="SCJ86_001876"
     CDS             complement(9345..9629)
                     /locus_tag="SCJ86_001876"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291219.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEG1169414.1"
                     /translation="MAKGPLITRSELRKRQQAQASESLKKQRKAETAYQQEEKKIASF
                     YRKESKKNKPITKTRISEREKTTKWNSFLMKSLIIVILMLCVVFLAIAFI"
     gene            complement(9642..10205)
                     /locus_tag="SCJ86_001877"
     CDS             complement(9642..10205)
                     /locus_tag="SCJ86_001877"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330027.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX hydrolase"
                     /protein_id="HEG1169415.1"
                     /translation="MLNNEQFEEKTISRKEIYSGKIIDVAVDEVRLPDGGTSKRELVF
                     HPGGVGIIAFDEQDRLLLVKQFRKPLEKVILEIPAGKIDPGEGQNPEMTAARELEEET
                     GYRAKSLSHLTSMYLSPGFANEVLHIYHAQGVEKVENPLAQDEDEVLELYHLTLEEAQ
                     QAMKDQLICDAKTIYAIQYWELLTKGK"
     gene            10433..11095
                     /locus_tag="SCJ86_001878"
     CDS             10433..11095
                     /locus_tag="SCJ86_001878"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318486.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-bromo-4-chloroindolyl phosphate hydrolysis
                     family protein"
                     /protein_id="HEG1169416.1"
                     /translation="MIKRNWPWLLALVLLFLFVRNNSFSFVLGLLLIGVVLVLWGLFG
                     SRRKKGKQEEIPALTDELETHYEKSGMTANEISFFRQTMNQTKNEINQLQMNMQQTAK
                     LKAIDLRHDPVKAAKALFKELVKEPNRLHEASQFLYTHLPNLVDLTNKYNEINDHEIK
                     NKQTYEKLEESAQIIDQLSALIAQDYQKFVAEDLDDIDVEISVAKQSLKRDNKYEPEQ
                     KD"
     gene            11219..12415
                     /locus_tag="SCJ86_001879"
     CDS             11219..12415
                     /locus_tag="SCJ86_001879"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339113.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="toxic anion resistance protein"
                     /protein_id="HEG1169417.1"
                     /translation="MDHKPTETKDVTPVNDTLEDLLNNPFSTPTDTLTTSQQAEIDQL
                     QEQQTAARLIDKLPAERQEQAKQLAAKIDANDAQSVISYGSAAQAKLSEFSQSMLNHV
                     QAQDIGPVGDSLTELMYRLQEANPDELRAGEGNIFQRVFGKVKQSIYEVTAKYQKIGA
                     QIDKISVKLDKEKDGLLKDNLMLEQLYQKNKDYFDALNIYIAAGELKMEELQTTIIPE
                     AMKRAEETGDQMDVQIANDYTQFLDRLDKRTHDLRLARQITIQQAPQIRLIQNTNQAL
                     AEKIQASIATAIPLWKNQVVIALTLLRQKDAVTAQRQVSETTNDLLKKNSEMLKISAI
                     ETAKENERGIVDIETLQTTQNDLIETIQETLRIQKEGKEKRRHAEVELGHMEEDLKQK
                     LLDLTQ"
     gene            complement(12516..13892)
                     /locus_tag="SCJ86_001880"
     CDS             complement(12516..13892)
                     /locus_tag="SCJ86_001880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631307.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HEG1169418.1"
                     /translation="MDLFRKKEINLNHTSEMKKELKTSDLIMLGIGAIIGTGIFVVTG
                     VAANQNAGPALSLSFVLAAIVVILSGLSFAEFASRVPVIGGPYAYLYVVFGEFAAWLT
                     GWLLIGEFLLAVSSVASGWSGYMQGFLKSLGAELPQALTGGYNPENGTYIDLIAVLVV
                     IFVTYIVSLEAKKALRLNNAMVYVKFGIIALFIIVGIFFVKPDNWQPFMPFGFSGVLD
                     GAALVFFAFLGFDAVAMAAEEVKNPQKDVPRGIIGSILIATVLYIIVTLILTGIVPYT
                     ELGVNDPVAFAMRYVGHGTVGAVIAVGAILTLLTVTISMMYSLARLLFAVSKDGLLPK
                     FMTEIDQKHRTPKKATYAAGVAAVFFAGFFPLNVLAELTNIMALAYLMLVSLGLLKLR
                     KMFGKPKAGEFKVPFVPLLPIVSILTCIVLMLRLQTVTWIVFGIVMVIGLIIYFGYGY
                     GHSKMNEK"
     gene            complement(13929..14795)
                     /locus_tag="SCJ86_001881"
     CDS             complement(13929..14795)
                     /locus_tag="SCJ86_001881"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291228.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucose transporter GlcU"
                     /protein_id="HEG1169419.1"
                     /translation="MEILIALVPMLAWGSIGLVSGKIGGDANQQTLGMTIGAFLFSLV
                     VFFVVSPVITPWIFLIGFLSGLAWSVGQNGQFHGMKYMGVSVGLPLSTGFQLILNTIA
                     GAVFFHEWTQTKDYVYGIIALALLVSGAYLTARQDDEGKIDTDNKMLDFGKGFRALIF
                     STIGYGVYTIIVNWANLDAMSIILPQSIGMILGASFFAFRKVKVDQFVWKNMICGLLW
                     GLGNICMLLTVKSLGLAVGFSLSQMGIIISTLGGIFILGERKTKKELIYVIVGCLLVI
                     LGGILLGYMKTV"
     gene            complement(14884..15909)
                     /locus_tag="SCJ86_001882"
     CDS             complement(14884..15909)
                     /locus_tag="SCJ86_001882"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374966.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HEG1169420.1"
                     /translation="MNFFKYLKDQWTLILGWLFFIALTIFVMWLAPNIKVDLSTIAYL
                     ALIEGVFLIFYLLGGYLSKRRWWGKLADIQKTSPLQNYLSGGRTEEEKLVEEYINQLI
                     REHQEVMQSAISNQQDQKDYIDSWVHEIKVPLSASKLLVRSIEFDIDDQKYTLLENEL
                     SKIDEYVEQVLYYARLDSFSRDYLIQEYGLKGIIQPVIRSQANYFIQKNIRYEIVGED
                     HTVLTDAKWIAFIFNQLLSNAIKYTPDHGNIIVSIEKEAQGVSLSVKDSGIGIPAEDL
                     KRIFDKGFTGRNGRLSKTHSTGLGLYLAKNLAEKLGIHLTAESTEGKGTTMTLFFPIL
                     NFYNEKR"
     gene            complement(15927..16601)
                     /locus_tag="SCJ86_001883"
     CDS             complement(15927..16601)
                     /locus_tag="SCJ86_001883"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003128479.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HEG1169421.1"
                     /translation="MAKIMVVEDEEIIRQLIMEELEKWQFETFGTTDFNQVFSDFERE
                     EPQLVLLDINLPVLDGYYWCQKIREVSKVPIIFISSRNTNMDMIMAMNMGADDFVTKH
                     FQIDVLIAKINALLRRSYNYTELSSEMMSHNGITLNVDNGSMEINGEIIDLSKNEYRL
                     LFILMKHNGKILSREKLLRALWDDERFVDDNTLTVNINRLRRKIEQAGIQGYIETKVG
                     QGYIVP"
     gene            complement(16785..17678)
                     /locus_tag="SCJ86_001884"
     CDS             complement(16785..17678)
                     /locus_tag="SCJ86_001884"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291235.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class C sortase"
                     /protein_id="HEG1169422.1"
                     /translation="MKKKIGNRPQTQISLLLQLFMAVMFLAGAAVFTYPFLADALSNY
                     LDQRRIENYQKQLERDKEEKHEQQLAVHEKKNQTLAHTSVIPGMGQVKDPFEQAVRNV
                     QNPGKEYYEQHMIGAIYIPKINVSLPLFDETNDLLLDRGATVLQGTSFPTGGESTHSV
                     ITAHSGIAEKKLFTDLEEMEQDDRFYLEVYGRMLAYEVVEKIVVLPTKTNTLAIREKQ
                     DLVTLITCTPYTVNTHRLLVTGRRVPFTEEVSSKIEQTKKYHLYRLLILLLGILLVLT
                     LFGYWCYRKFKRQKQRKKNNR"
     gene            complement(17752..19449)
                     /locus_tag="SCJ86_001885"
     CDS             complement(17752..19449)
                     /locus_tag="SCJ86_001885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350040.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SpaH/EbpB family LPXTG-anchored major pilin"
                     /protein_id="HEG1169423.1"
                     /translation="MNKKMIVTGMSVLLLVTAGIGIFGNGKLVKAEEVAQKPSEVTIT
                     LHKKGFSSVPEERPNSGLVSTDFGEENIPGVDFDLFDVTEVYYDLIRDNPLTPEREDG
                     LNSAEAIEWIQKRHTESWFLKYRLTSIDKQTTNEAGEAVFSTVQVTEEAPSSRDKVYL
                     FLETYSPAHISRIASPMVVMMPVMMPDMVDGVWDGSTWKDTYNTDVHLYPKNEIREAD
                     KQMNVEESDLRQVTIINEAGEQETISYIDLERGKTASYTITAPIPYFIDSVLENGSAV
                     IKNYKITDTPTVGLTYYDQEIEVRAGETILTKGQDYIVEVVSNGFVVTILTEENGVAK
                     VDTLGRLADARGGDLTITYNLKVSTELEADDFHNNTAVIEIGRNDEFDYEEGVEPPEK
                     VTTGGRKFEKYDASSSELLKDARFELWNEDRSEYAIFYKGESPLAVYESGADRIEWAT
                     SGQATEFVADGNGYFEVQGLDYGTYQMKETMAPEGYVLPTGEAAFTEFIISYGSYNEE
                     IQIVGVENPGPERVPNMKRGSLPATGGNGLLAFLLIGISLMIGAYSWYRKSKMKSEV"
BASE COUNT         5684 a         4189 c         3445 g         6157 t
ORIGIN      
        1 tctttccctc ttttttttgt ttattcgaat tctttttctt ccagacggaa taacggactc
       61 atcggtgtat tttcatggat gcgtttgacc gcttcaccca ttaattctga acatgtaacg
      121 attgttaagt tttctggatg acgattttct tctgtgaaaa cagaatcagt gatacaaata
      181 tctttgattg gtgcattgtc taaaatactt ttcgcatgat cggacaataa tccatgtgac
      241 gcacaggcat atacttcttt tgcgccgttt tcttttaata catttgctgc tcgtgcaagc
      301 gttgctcctg tgttcaagat atcatctacc atgatacatg tctttccttg aacatcaccg
      361 atcacataac caccctcagt tgaagcatca tctgcatgat ctacgatggc taatgtgctg
      421 ttcaaatatt ctgaaagact acgtgcacgc tgaacaccgc tgttttttgg cgaaacaact
      481 acgatgtcat ctcccgtcag acctaattga cggtaataat gagcaaacaa cggcatcgta
      541 aataaattat ctacaggaat atcaaagaat ccttgtactt gaaccgtgtg cagatctaaa
      601 gtcagtactc gagttgcccc tgcttcttcg atcaagttag cgatcaattt cgcagtgatc
      661 ggttcatgag gtttcgccgt gcggtcttgc ctagcatacc catagtaagg caatacgaca
      721 ttgatcgttt tcgcacttgc tcgcttcatt gcatcgatca tgatcagtag ttccatatag
      781 tagtcattga caggtgcatt cgttgattga acgatataca catgatcccc tcgaatactt
      841 tcttcgatat tgatggaaat ctcgccatca ctaaattgct tcacgacgct tttacctaat
      901 tctgttccga acactttcgc gattttttca gctaatggac ggttgccatt caaactaaaa
      961 atcttcaacg tgtcatcctg atacttatta ctcatgtagt cctccccaaa acttctctat
     1021 tcttcgatag tttaacatat ttttccaaga ttttctgtta atttcagcaa attcttaagg
     1081 aagtgaaact gttccgttac tccaactggc aggaatgact ttatgataat cgccccagtt
     1141 gtcgtgtact ttgtaataac gttctcctgt ttcagtttca aaataagcgt acgctgtcac
     1201 ccaatgattg ccatatgtac tgccaagtac tttcaaaata ccaatcatca caggtttgcc
     1261 ttcacgtata cgttttgtag ctcgctgcca tgaacctaga agagttgctc gtgcacgctc
     1321 atgattgcca ttcttcttta aaaaactaga aatccctgta ctaacctgaa aaggaatcgt
     1381 aggaagtcct agtggttgga tctggctacg caatgccttc gtcaaaacca acgaatcagc
     1441 cgtatttttc ttgcgaatct catttggaag catccacccc ttacgatagt cttgataata
     1501 ggcaaggaga actgctgccg cgtaagtacc acaaagatag cctgaggggg tagcccattt
     1561 cttatacctc aaaaaacgtt cttctggcaa tcccacccag caatttgcga gttcctttcc
     1621 tgaccttttt tcttcgatca aaacgggaat atcttctttt ctgttgatcc atctagataa
     1681 ctcgattggc tcttcattga tcagcttcca ttttttacaa gaaagaccct cttgataagt
     1741 atattttgct ggagaatatt ctaccggatg tcctgattga tcgataatca cataactttg
     1801 atcttcaaaa accagagcta aaaaaagcaa tttacttttt cttgactgca gctgctgagt
     1861 gctggctaat tttttaattt ttttatctac tttgaaatat aaaggtttat ccactacttg
     1921 attcagttgg gatcgcctcc tttttttcat tataagtgaa ggattgaaaa aaacaaatca
     1981 gtttgattgt tcgaattttc aattttttat gttctatttg ttaattcctt cattttgtaa
     2041 gataatgggc acacgaaaca aaaaaggagc gatcgatgaa tgaacaaaga acgattgaaa
     2101 caagatttct atgaagcagt caatgaagaa tggcaaaaaa cagcaaaaat ccctgcagat
     2161 aaaccagcta caggcggttt ccaagattta gttgacggga tcgaccaact tttgatggga
     2221 gaaacagacg atctgcttgc ccatccagaa aaagtgtctt cagaaaaaat gaaacatttc
     2281 cttgcatatt atcaactggc aaacgattat gaccgaagaa atgagttgca tgctcaaccg
     2341 cttcaaccta taatcgaaaa agttgaatca ttgcagtctt tcgacgaatt gaataagcaa
     2401 tttccagaat ggctgttgga tacattacca ttgccattta atctggatgt agatgcagat
     2461 atgaagaatg cgcaaacgaa cgccttcttt gcttatccgc cttctttatt ccttccagat
     2521 aagacgtatt atgcatcaga ccatcccaac ggtgcacaac taataggcgt tttctttgat
     2581 atggctgttc agttattaga gcttgccggc aaagaaaaaa gtcaggcaga agcaatcacg
     2641 gaacaagcag tgcaatttga taaattgatt gctcctcatg taaaaagtgc agaagaaaac
     2701 gcagattaca gcaaaatgta taatccgcaa tcttttgata catttattaa atacacagat
     2761 aaactggact tgaaaaaact tgtcacgggt ttgattggga caactccaga taaagtgatc
     2821 gtaactgatc cagtgtactt cgatcatttg aatgacatcc tgacagaagc tcattttccg
     2881 ttattcaaaa gctggatgat cattaaaaca gtacgttctc ttagtggtta tctatcagaa
     2941 gaattccgcc aagtcagcgg aatctattct cgcgtattgt ctggaacaga cgaagcaatg
     3001 cctcagaaaa aagcagcgta ttaccttgcc tctggacaat tcgatcaagt agttggcgat
     3061 tactacggaa aaaaatattt tggcgaagct gctaaaaaag acgtagaaca aatggtgaaa
     3121 aaaatgatcg ccgtttataa aaaacgtcta gaagaaaatg actggcttgg ggatgcaaca
     3181 aaagaaaaag ccgtcataaa attaaataac ttaggtatcc aagtaggcta ccccgataag
     3241 atcccagcat tattcgacca gttcaaaaca gtacctgcag atcagggtgg tactttgctt
     3301 tcaaatactc ttgttttcag caaactcaca ttaaaagatc gtttcagcaa atggaacaaa
     3361 ccagtggatc gttcagaatg ggaaatgagt gctgacacgg tcaatgctta ttatcatcca
     3421 tttcggaatg tgatcgtctt tccggcagcg atccttcaag caccatttta cagtctagaa
     3481 caatcaagct ctgctaatta tggtggaatc ggagctgtta tcgctcatga gatttcccat
     3541 gcatttgata ataacggcgc tttatttgac gaatatggca acttgaacaa ctggtggaca
     3601 gaagaagatc ttacacaatt tcaaaacaaa gcacaagcaa tgatcgaaga attcgatggt
     3661 ctgccttttg ctgacggaaa agtaaacgga aaattaaccg tttcggaaaa tattgctgat
     3721 gcaggcggtc taagctgtgc attggaagca gcaaaaacgg aatcagatct ttctatcgag
     3781 gaattctttg tcaattgggc aacgatttgg cgtacaaaag caaaaaaaga ataccaacaa
     3841 cttttactgc aaatcgatgt ccatgcccct gctaaactcc gagcaaacgt gcaaccaaaa
     3901 aaccttgacg aattctatga aacatttggt atcacggaac aagacgctat gtatctagcg
     3961 ccagaaaaaa gagtgcgaat ctggtaagag gttgtgccag aagtggtcag cttcaagaaa
     4021 taagaaggaa tttgggaaaa tcgcttctca aatttttgtg acatttcggc ttatttttcg
     4081 aagaagttac ttctggaata ccatgtatta gaggttgtga aaaagctgct tagtttcaag
     4141 tagtaagaaa aattttgtga aaatggcttt ccacattttt gcataaattt gacttactac
     4201 cgagaaactg gcttttgaac accgtttatc gagaggttgt gaaatcagcg tcctgacctg
     4261 agtattagag aaaaaaacgg aaaaatagtt cctcatattt tgccgtttgt tgagctaata
     4321 ccgagggtca gctgattgaa caccgtttag tggagagatt gtgaaaaagc tgtcctgcat
     4381 caagtaataa gaacagccaa tcaaaagcag caaggagaac ctcatctata aaaagataga
     4441 aggaagtgag acatctattg gtctcatttc cttctatttt tactttcctc agtcaattgt
     4501 tttacatcat aaaaggagaa attccactta tttttttcgc tgtttcatga cataaacaac
     4561 tacaccgatt aacaaaattc ctccagctaa tgctagcaac gggttattaa attctcccgt
     4621 ttttgggagt ccttgagtgc catttttttc actttctttt gctgtcctat ccggatgaga
     4681 aggttttcca tttggttttc catttggtga ttcatttcct ggattttctc tttctggttt
     4741 gtcatttccc gggggcacat cggcagtttc tcgcacagtg atcgcttttt gctgggtaac
     4801 agattctgta tgagcagacc atttttcccc atcaaatatt tccgaacgat aagtgacttc
     4861 tagcgtatat tctccttttg aaggtgctga aaaaatagcg gtatgattag aaaattgagc
     4921 acttgactgt gtttcaacga tttggatctt ttctgggaaa taacgcatat cttcaaagac
     4981 aggcgttcct tgatttggtg tgaaagttga cacaaaaact gtaaaaggct tcagttcaat
     5041 gatatttcct tctgcattct ccgccacacc atttgcttct tgtataataa tcggattatt
     5101 ttgttcattt ggttttgtct gatttggttc ttgtacgatt gcatactctg gatattccga
     5161 agacggtgag gtttctccgt caaaaatagc acctacagat aatggaagac catccactct
     5221 ttccttttct gtttgacgat tccccggttt aatctgcatc agtccatgaa catggatact
     5281 tccattttca tagcgcgtaa caggctgctg accttctttc gaacatgtat ttagagactc
     5341 gatccaatct tttgatatgg tatgtccaag agtattttct gattgtttct ctcgttcatt
     5401 ccaatagtag ttcaacgagt ggtagatttg atcttcgttt tgattcaatg ggcggatctg
     5461 ctctctcatc tcaagattct ctgttcggaa agataagata ccagaagcat ggtagtctgt
     5521 gtttcctgct gaagaggtat aaagagccac attatatgaa ccgttattaa aacttgtact
     5581 gctattgacg ataatatctg ttccgctatt ggaatcgatt cctttcgcaa gattttcgaa
     5641 agacaaacta ttgatcaatt catgagggcc cgttaaagaa gaaccgccca ttttgaatcc
     5701 attaccgtta ccttctccct caattcctgg tacccagccg tttcgataag caactgagtt
     5761 tttgatgatt acttttccga tcgaacctgt ttcattttta gcaaaaaggt cccaaccgtc
     5821 atctgcatta tgatacgcga tacaaccgtc aaatacattt ccttctccta ttgtcagttt
     5881 ggccgcaaat ccatcggcat cttcaaatcc tggatcggca ttttcaaaag atgtacagtt
     5941 cttgactaag ttatgcgcgg gccatgcttc gaaaggatca tttgagctac cgctgatttg
     6001 gattcctgta tttccattcc gataagcatg aatctcttcg atcaaattat gatttccaga
     6061 aatctgtaat cctttttgca tagctgcact atttgtaatg tcaaaccctc gtaaacccca
     6121 gtagtgactg aacagtgtca tcccatttcc agttccttta aaatcaagta caggacgctt
     6181 gttttctgga tttttctctg caatcaactg aatcgggttt tctcttgtcc cgttaacgcc
     6241 ttttggaatc gtcaatcctc tttcgaagga ataagttcca ggcgccatga cgattgtctg
     6301 cccagcatat gcgtatctca atgcttgtgt cagagataat ggattttgtc tgcttccgtt
     6361 tcctgcatct gacccttctg gtgttacata aactgtcgaa tatttaggtt tacggtagtc
     6421 cactgtatga agaatctctt ttgtttcata actagtcatc tctacgtatt cttctggcgg
     6481 atagttttta tctggcgtaa aactgaaagt aaattcgttc gttccttcca tcagttttgt
     6541 ctgataatca aattcccgat tcgcagtgat agccaagttt tttgcgttct taagcacctc
     6601 tcctgttgct ttatttttaa ttgtcaataa tccgtcagca tttgctctaa aggacatagt
     6661 atgatcttgg tttcctgaat gattactaga ggtcacagtg taaatcggat caatcaagcg
     6721 agtaggtctt tcttgcccag gaggatctgt cgcaggatct gagtaagcaa cttcgattcc
     6781 ttctaccttg atcgtcatat tccttgctgc agcaaatcct gcataaataa ccgattcatc
     6841 tgaagcgtat aatttttccc agtcatacat gatgtcagaa gccatttctt ctcctgtttc
     6901 aggatcgtaa taatatgact ctaaaccagt attcgttttt ttcatctcta cgtccagttt
     6961 agtaatcgag ttcgtggccg gaaacaaatt cccatgaccg tttccgaata tattataaga
     7021 acctgtttct ttttccaaga accctgctga agtctctaaa ggtgttgttt ccactgagac
     7081 actcccagga gctggcgcag cttgcggatc ttcagatgat attcctgtga ttgcccgtgt
     7141 ccccaatcca agtcgcatcg tatatttgct gccgctcgtg ttgaccactt cttgtctatc
     7201 cgagtcccag acatattcga tccgactgcc tagtatggca aatgaatttg aataaaatgc
     7261 ctgcccaaac aaatgatttt ttggtactgc gtctcttacc attaaagcaa atccttcttg
     7321 ggcattcgta taggtccaag attctatttc gatcgtcgcc cgcatcctaa agttgatgtc
     7381 tttggggatt gcttgataat aataggtcat tccatctgat ccgcttgact ggaattttcc
     7441 gccgttattc gtggaagaga gccgcacgcc gtcttctaat ggttcaatgg catacctact
     7501 agcacttgtg ctttctccga aaacaacccc tttccactct tcatttgctt cttcagattg
     7561 cttttttgtc ttttcatttt ctgattcttt catcatagga tcatttcctt cttccgtatc
     7621 catatcttca ctagttcttg gtacttcctc cattgttgac atcccttcaa tgttcacgtg
     7681 tgcattttct tcgttcacag actgttgttc gttagcagca acttcaacga cagaaattaa
     7741 aaccggggta ctgagcataa ggagattcat cattagcgca ctgtatttta tacgtttttt
     7801 taacaagtgt attcctcccg taacaaatta gaaagcgttt acttaaagta ttacctaatt
     7861 tatttttact gtcaataaaa aacaaaatta taataagtgt attttataaa aagaaaaaat
     7921 ataacttaag ttgttgataa taaagagatt tattaacgta aaaagaataa gtttttttaa
     7981 aataacgtat aaaaaagagc ataaaacaaa gtcgtttgtt ttatgctctt cttttattat
     8041 tttgtgcttt ttattttatc aaatcacttt gtttttttat aaaatttctg cgcctagtgt
     8101 ggactcaaaa tgtctcaatg cccattcatg tccaacggga tcgaaacttg ccacggcatc
     8161 tttgaaaata tggagtttgt atcctagatt atacgcatcg actgctgtat gcaacacaca
     8221 gatatctgta catacgccgg tcaaataaat atctgtgatc tggcgttctc gcaaacggat
     8281 gtctagatct gttccactaa aagcggaata atgccgtttg tctatccaat aaacattttc
     8341 ctcttgctca tagtcttggt ataaactttt taaagagccg tacaattccc gtccacccgt
     8401 tcctatcaca ttatgcggag gaaataagcg gttctccgga tgaaaggaat cttttggatc
     8461 atgcgcatca atggcaaaaa cgacaaaatc cttttgatca ataaacttct ttgtatgagc
     8521 agtcagcttt ttttcaatta cttgtcctgc tgtcccagta gtcagctttc catcctctgc
     8581 aacaaaatca tatgtgtagt caatcgagat caatgcgttc atcttagtcc tccttatacg
     8641 agatgtttga caaattcgat caccatttct gctgatcgtt tgcctgcgtc ttcgataaat
     8701 tcgtcaaagc tttgtgtcgc cgagtgatct gctgtgtcgc tcattgcacg tacgatcaaa
     8761 aatgggatat tgaactgacg agctgtttgt ccaacagcgg ctccttccat ttcgcaagct
     8821 aatgcttcag ggaaattcgt taagatttct ttgattttat ctggcgagtc gacaaatgta
     8881 tcacctgtaa cgatcaatcc ttcttttgcg ttcaaattcg ttgcttttgc tgctttaacc
     8941 atttcactgc gcaaatatgt gcttgcttca taatatagag gcattcctgg taattgtcct
     9001 ggtttgtagc caaatcctgt tgcatctgca tcaaaataag ctactttgtc tgatataacg
     9061 atgtcaccta cttgtaagcc ttcaccgatt ccgccagctg aacctgtatt gatcaccatg
     9121 ttcacgccgt attgttggat caacagactt gtggtaatag atgctaagac ttttcctata
     9181 ccggaacgta cgacgatcac ttcgtggtta cctaatgaac ctgaaataaa taaagcgcct
     9241 gcgcgttccc atgataatgg ttcgcttagg ttctctctca aaatttttac ttcttcttcc
     9301 attgcgccga taataccgat tttcatcttt ttaattcctt ctttctatat aaatgcgatt
     9361 gctaaaaaaa ccacacataa cattaggata acaataataa gagatttcat caaaaaagag
     9421 ttccatttgg tcgtcttttc ccgttcgctg atccttgttt tcgtgattgg tttattcttt
     9481 ttgctttctt tacgataaaa gctggcaatc tttttttctt cttgctgata agctgtttct
     9541 gcttttcgct gtttttttaa tgactcgctt gcttgtgctt gttgtcgctt gcgaagttcg
     9601 cttcgcgtaa taagcggtcc tttggccata agccgatccc tctactttcc tttggtcaat
     9661 agttcccagt attgaatcgc ataaatcgtc tttgcatcac aaatcaactg atctttcatc
     9721 gcttgttgcg cctcttcaag agttagatga tacaactcca gtacttcgtc ttcatcttgt
     9781 gctaaaggat tctcgacctt ctctacccct tgtgcatgat aaatatgaag gacttcattt
     9841 gcaaaacctg gtgaaagata catcgaagta agatggctca gcgacttcgc acgatagcca
     9901 gtttcttctt ctaattctct agctgctgtc atttcgggat tttgtccttc tcctggatcg
     9961 atttttccag ctggaatctc caaaatcact ttttctaaag gttttcgaaa ttgtttgaca
    10021 agaagcagac gatcttgttc gtcaaaagca atgatcccga caccaccagg atgaaatacc
    10081 agttctctct tcgaagttcc tccatctggc aaacgaactt cgtctacagc aacatcaata
    10141 atttttccgc tatagatttc tttgcgactg attgtttttt cttcaaattg ctcattgttc
    10201 agcatatctt ctcctgcttt ctttgtaatc ataactgctt ttttttcatt tttcaatttt
    10261 tctcaaaaac tccttattag tttaccatag ataaacctta gattttctct aaatatagaa
    10321 aatccttctt tagtccgata ctctttctag tagtccgtat gctaaaataa gtatgtaatc
    10381 gaataacgaa aagaattcaa tactcaacga taaatgacat ggagggttta ttatgataaa
    10441 acgaaattgg ccgtggcttc tagcattggt cttgctattt ctctttgtcc gaaacaatag
    10501 tttttctttt gttctaggac ttttattgat aggtgtagtt ttagttttgt ggggactctt
    10561 tggttcgcgc aggaaaaagg gaaaacaaga agaaattcct gcactgactg acgaacttga
    10621 aactcactat gaaaaaagcg gtatgacagc gaatgaaatt tcattttttc gacaaacaat
    10681 gaatcaaaca aaaaatgaga tcaatcaatt gcaaatgaat atgcagcaga cagccaaact
    10741 taaagcaatc gatctccgcc atgatccagt caaagcagcg aaagctttat tcaaagaatt
    10801 ggtgaaagaa ccaaaccgac tgcatgaagc aagtcagttt ctttatacgc atctgccgaa
    10861 tctagttgat ttgacaaata agtataatga gatcaatgac catgagatca aaaacaaaca
    10921 gacctatgaa aaactagaag aaagcgcgca gatcatcgac cagctgtcag ctttaatcgc
    10981 acaagattat caaaaattcg ttgctgagga tctagatgat atcgacgtag agatatctgt
    11041 tgcgaaacag agcttaaagc gtgataataa atatgaacca gaacaaaagg attaactctt
    11101 ttgttcataa aagaagactc ggaacattca gatgatgtct ggctgtttcg agtacttttt
    11161 ctaaagtaga gacattcatg taagaatcga gaacagattc aacaaggagg aattttttat
    11221 ggatcataaa ccgactgaaa caaaagatgt tacaccagtt aacgatacac ttgaagattt
    11281 attgaacaac ccgttttcaa ctccaacaga tacgctgacc acttcacaac aagcggagat
    11341 tgatcagctc caagagcaac agacagctgc tcgtttaatt gacaaactac cggcggaacg
    11401 ccaagaacaa gctaaacagc tagcagcaaa aatcgatgct aatgatgcac agtctgtcat
    11461 cagctacgga tcggctgccc aagcaaaatt aagtgaattc tcacaatcga tgttgaatca
    11521 tgtgcaagca caagatattg gtcctgtagg agattcctta actgaactga tgtatcgtct
    11581 ccaagaagcc aatcctgacg aactgcgtgc cggagaaggg aacatcttcc agcgtgtctt
    11641 cgggaaggtc aaacaatcga tctatgaagt gacagcaaaa tatcaaaaga ttggtgcaca
    11701 aattgacaag atttcagtga aattagataa agaaaaagac ggtctgctaa aagataatct
    11761 gatgttagaa cagctttacc agaaaaacaa agactacttt gatgcactga atatttatat
    11821 cgctgctgga gaattgaaaa tggaagaact tcaaacaacg atcattccag aagcgatgaa
    11881 aagagcagaa gaaactggcg atcagatgga tgttcagatt gccaatgatt acacgcaatt
    11941 cctagatcgt ctggataaac gtacgcatga tcttcgctta gctagacaga tcacgatcca
    12001 gcaagcacca caaatccgtt tgatccaaaa cacaaatcag gccttggctg aaaagatcca
    12061 agcatctatc gctacagcca ttccgctgtg gaaaaaccaa gtcgtgatcg cattgacctt
    12121 gcttcgtcaa aaagatgcag taactgctca acgccaagtc tctgaaacaa caaacgactt
    12181 attgaagaaa aattcagaaa tgctgaaaat ctcagccatc gaaacagcga aagaaaatga
    12241 acgtggaatc gtggatatcg agacccttca aaccactcaa aatgatttga tcgaaacaat
    12301 ccaagagaca ttgcgtatcc aaaaagaagg aaaagaaaaa cgtcgtcatg cagaagtaga
    12361 gcttggacac atggaagaag atctgaaaca aaaactgttg gatttaaccc aatagatcac
    12421 ctttcgcttg ttactaaaac aaacgcccaa caacagttta ttacaactgt tgatgggcgt
    12481 ttttcttatc tttcttctaa cttctccatt ggagtctatt tctcattcat cttgctgtgt
    12541 ccatagccat agccaaaata aatgatcagt ccaataacca ttacgatacc gaacacgatc
    12601 catgtcacag tttgcagacg aagcatcaat acaatacaag tcaagatgga gacgattggc
    12661 aatagcggaa cgaatggcac tttgaattct ccagcttttg gtttgccaaa catttttctc
    12721 aacttcagta agcctagact aacaagcatc aaataggcga gcgccatgat attcgttagt
    12781 tccgctaata cattcaatgg gaaaaatccg gcaaagaata cagctgccac accagcggca
    12841 taagtcgctt ttttcggtgt acgatgcttt tgatcgattt ctgtcataaa cttaggaagt
    12901 aagccatcct tgctgacagc aaacagtaaa cgagcaagtg aatacatcat cgagatcgtt
    12961 accgtcagta gcgttaaaat tgcccctaca gcgatcaccg cgcccactgt tccatgtcct
    13021 acatacctca ttgcaaaagc cactgggtca ttgactccta gttcagtata cggaacgatt
    13081 cctgtcaaga tcagcgtcac gatgatgtaa aggacagtag caatcagtat cgaaccaatg
    13141 attcctcttg gcacatcttt ttgcgggttt ttgacttcct cggctgccat tgcaacggca
    13201 tcaaatccca agaaagcaaa aaacacaaga gctgcaccat ctagcacacc ggaaaaacca
    13261 aaaggcatga atggctgcca gttatcaggt ttaacgaaaa atattcctac aatgataaac
    13321 aacgcgatga taccaaattt gacatatacc atggcattat tcaaacgtaa tgctttcttg
    13381 gcttcaagcg acacgatgta agtgacgaat atcactacga gcaccgcaat caagtcaatg
    13441 tatgtaccat tttccggatt gtacccgcca gttaatgctt gtggaagttc ggcccctaaa
    13501 cttttcaaaa aaccttgcat atagccagac catccggaag caacagagga aaccgctagt
    13561 aaaaattccc cgatcaacaa ccaccctgtc agccaggcag caaattcacc aaatacaaca
    13621 tacagatagg catatggccc accaatcact ggcactcgcg aagcaaattc tgcgaaactt
    13681 agtccagaca aaatgacaac gattgcagct aagacaaatg aaagtgacag tgctggacca
    13741 gcattttgat tggccgcaac ccctgtcacc acaaatattc ccgtaccgat gatcgcacca
    13801 attccaagca tgatcaaatc agacgttttt agttcttttt tcatctcact cgtatgattg
    13861 agattgatct ctttcttacg aaacaaatcc attgaagttc ctccccattt taggttggaa
    13921 aacagcgatt aaactgtttt catataccct aaaaggatac cacctaaaat gactaaaagg
    13981 caaccaacaa taacataaat gagctctttt ttcgtttttc tttcgccaag gatgaaaatc
    14041 ccacctaaag tagagataat gatccccatt tgtgaaagtg agaagccaac agctaaacca
    14101 agtgatttga ctgttagaag catgcagatg tttcctaagc cccataataa tccacagatc
    14161 atgtttttcc acacaaactg atcgacttta actttacgga aagcaaagaa gcttgcgcct
    14221 aagatcattc cgatactttg aggtaagata attgacatcg catccaaatt cgcccagttg
    14281 acgatgatgg tatatacacc ataaccaatc gtagagaaga tcaatgctct aaaaccctta
    14341 ccaaaatcaa gcattttatt atctgtatct atttttcctt cgtcatcttg tcgggcagtc
    14401 aaatacgcac cgcttacaag aagagctaaa gcaataatac cgtatacata atctttcgtt
    14461 tgtgtccatt catggaagaa aacggctccc gcaatcgtat tcaaaattaa ttgaaaacca
    14521 gtagacaatg gtagccctac agaaacaccc atgtacttca ttccatggaa ctgtccattt
    14581 tgtccaacac tccaagctaa gccggataaa aatccgatca agaaaatcca aggggtgatt
    14641 acaggactaa caacgaaaaa gacaactaaa gaaaacagaa atgcaccgat cgtcattcct
    14701 agtgtttgtt gattggcatc gccaccaatt ttaccactta ctaaaccaat actaccccat
    14761 gccaacatag ggactaatgc tattaaaatt tccataacca atctcctttt atttcacatg
    14821 ataagaaact tatcagcgaa aacgttaaca acacaagcac tttttttcgt ttctaacgat
    14881 tttctaacgt ttttcattat aaaaatttaa gattgggaaa aataaagtca ttgttgtccc
    14941 ttttccttca gttgattcag ccgtcaaatg gatacctaat ttttcggcca gattttttgc
    15001 taaataaagt cctaatcctg ttgaatgtgt tttgctaagt cgaccatttc tcccagtaaa
    15061 tcccttatca aagatccgtt tcaagtcttc cgctgggata ccgatccctg aatctttaac
    15121 tgaaaggctg actccttgcg cttccttttc tatgctcaca atgatgtttc cgtgatccgg
    15181 cgtatatttg atggcattac taagcaattg gttgaagata aaagcgatcc atttagcatc
    15241 ggtcaaaaca gtatgatcct caccaacgat ctcgtagcga atattttttt ggataaaata
    15301 atttgcctga ctgcgtatca caggctgaat gattcctttt agtccatatt cttggatgag
    15361 gtagtcccgc gagaaactgt cgagtcttgc ataatacaaa acttgttcta cgtattcatc
    15421 gatttttgat aattcatttt ctagtaatgt atacttttga tcgtcaatat cgaactcgat
    15481 cgaacgaact agcaatttag aagcggaaag tggcactttg atttcatgga cccatgagtc
    15541 aatataatct ttttggtctt gttgattact gattgctgat tgcattactt cttggtgttc
    15601 acggatcagc tgattgatat attcttctac cagtttttct tcttctgtcc tcccaccact
    15661 caaataattt tgcaaagggg aagttttttg tatgtcggct aattttcccc accatcttct
    15721 cttgctcaag taaccaccca gcagataaaa aatcagaaag acgccctcaa tcaaagctaa
    15781 ataagcaatt gtgctcaaat caactttgat gtttggcgcc agccacatca caaagatcgt
    15841 caatgcaata aaaaacagcc atcccaagat caatgtccac tgatctttca aatatttaaa
    15901 aaaattcatc ttaaccgctc atcctttcag ggaacgatat atccctgtcc tacttttgtt
    15961 tcgatatatc cttggatacc tgcctgttcg atttttcggc gtaaccgatt gatgttgact
    16021 gtcaatgtat tgtcatctac aaatcgttcg tcatcccaca gtgctcgcag caatttttcc
    16081 cgacttaaga tctttccatt gtgcttcatc aaaataaata atagacgata ttcatttttg
    16141 ctcaaatcaa tgatctctcc gttgatttcc atacttccgt tgtcaacatt caacgtaatg
    16201 ccattgtggc tcatcatttc actagataat tctgtgtagt tgtaagaacg gcgaagtaat
    16261 gcattgattt ttgcaatcag tacatcgatt tggaaatgtt ttgtcacaaa atcatctgcc
    16321 cccatgttca tagccatgat catatccata ttcgtattcc tgctggagat aaaaatgatc
    16381 ggtactttcg atacttcacg tattttttga caccaataat agccatccaa aacgggaaga
    16441 ttaatatcta gtaatacaag ctgtggttct tctctttcaa agtctgagaa cacttgattg
    16501 aaatcggtcg ttccaaaggt ttcaaattgc catttctcaa gttcctccat gatcaattgt
    16561 cgaatgatct cttcgtcttc gactaccatg atttttgcca taaaaaatcc ctccatcttt
    16621 cttcttgcta ctacaatatc aaagaagccc ttgtgaaaca agttggctta cctggaaaat
    16681 gtcattttag caaacgttaa aaaaaactgg aaatatgcga tttaatcgcg tttgtctctt
    16741 ccaagcagct taaatttatt atgttattct taataattgg tctattatct gttatttttc
    16801 tttcgttgtt tttgtctttt aaattttcga taacaccaat aaccgaaaag cgttagcact
    16861 agcaatatac ctagaaggag tataagtaat cgataaagat ggtatttttt tgtctgttct
    16921 atctttgaag agacttcttc tgtaaaggga actcgtcttc ctgtcactag taatcggtga
    16981 gtgttcacgg tgtaaggtgt acaagtgata agtgtgacta gatcctgctt ttctctaatt
    17041 gctaatgtat ttgtttttgt tggaaggaca acaattttct caacgacttc ataagcgagc
    17101 atccgaccat acacttcaag atagaatcgg tcatcttgct ccatttcttc aagatccgta
    17161 aagagttttt tctcagcaat accgctatgg gcggtaatca cggaatgtgt actctctcct
    17221 ccagttggaa aagaggtgcc ttgcaggact gtcgctcctc tatctaacaa aagatcattc
    17281 gtttcatcaa ataaaggcaa gctaacattg attttcggaa tatagatggc tccgatcata
    17341 tgttgttcgt aatattcttt tcctggattt tggacatttc taaccgcttg ctcaaaagga
    17401 tctttgactt gtcccattcc tgggattact gatgtatgag caagcgtctg atttttcttt
    17461 tcatgcactg ctaattgttg ctcatgtttt tcttctttat cgcgttcaag ctgcttttga
    17521 taattctcga tccttctttg atccaaataa ttgctgagag catctgctaa aaatggatat
    17581 gtaaaaactg cagctccggc aagaaacatc actgccataa ataattgtaa taaaagactg
    17641 atttgggttt gaggtctatt acctattttc tttttcattt tcttcacctt cttatttaaa
    17701 aaaactgcag caacctttat aaaaccgatt gctgcagctt atttttgttg cttatacttc
    17761 agacttcatt ttagatttac ggtaccagct gtacgctcca atcatcaaac ttattccgat
    17821 tagtaagaat gccaagagcc cgttcccacc agtagcagga agtgacccgc gtttcatatt
    17881 cggtactctt tctggccctg gattttctac tcctacgatc tgaatttctt cattatagct
    17941 tccataagag atgataaatt cagtaaaggc tgcctcacct gttggaagta cataaccttc
    18001 tggcgccatg gtctctttca tttgataggt tccataatct aacccttgaa cctcaaagta
    18061 tccatttcca tctgcgacaa attctgttgc ttgtccgctt gttgcccact caattctatc
    18121 tgctcctgat tcataaacag ctaaaggcga ctccccttta taaaatattg cgtattcaga
    18181 acggtcttca ttccacaatt cgaatcttgc atctttaagc aattcactgc ttgatgcatc
    18241 atatttttca aactttcttc cacctgttgt gactttctct ggcggctcta ctccttcttc
    18301 ataatcaaat tcatcatttc gtccgatttc aataaccgct gtgttgttat ggaaatcatc
    18361 cgcttctagt tctgtggaaa ccttcaaatt atacgtgatc gttaaatctc ctccacgtgc
    18421 atctgctaat ctacctaaag tatctacttt ggctactcca ttttcttctg tcaaaatcgt
    18481 cacgacaaac ccgttgctta ctacttctac tatataatct tgtccttttg ttaaaatcgt
    18541 ctcgccggct cgcacttcta tttcctgatc ataataagtt agaccaactg taggcgtatc
    18601 tgtgattttg taatttttga taacagcgct tccgttttca aggacagaat cgatgaaata
    18661 aggaataggt gctgtgatcg tgtaagaagc cgtttttcct ctttctagat caatataaga
    18721 aatcgtttct tgttctccag cttcattaat aatcgttact tgtctaaggt cgctttcctc
    18781 tacattcatc tgtttatctg cttctctgat ttcgtttttc ggatacaaat gcacatctgt
    18841 attgtatgtg tctttccatg tagaaccatc ccacacgcca tccaccatat caggcatcat
    18901 cactggcatc ataacaacca ttggagacgc gattctggaa atatgagctg gcgaataagt
    18961 ttctaagaaa agatagactt tatctctaga agaaggtgct tcttccgtca cttggacggt
    19021 agagaatacc gcttctccag cttcattcgt tgtttgtttg tcaatacttg tcagtcgata
    19081 ttttaaaaac caactttctg tgtgccgttt ttgaatccat tcaatcgctt cggcagaatt
    19141 aagaccgtct tctcgttccg gtgttaaagg attatctcga atcaaatcgt aatatacttc
    19201 cgttacgtca aacaaatcaa aatcgactcc tggaatattt tcttcaccaa aatccgtaga
    19261 aaccaatccg ctattcggtc gttcttccgg aacagatgag aaaccttttt tgtgtaatgt
    19321 aatcgtcact tcacttggtt tttgtgcgac ttcttcggct ttgactagtt ttccgttccc
    19381 gaaaatccct attcccgcag taacgagtaa cagtacactc attcccgtga caatcatttt
    19441 tttattcatt tttttctccc cgttcttttt ttatt
//
LOCUS       DARBET010000049        19374 bp    DNA     linear   BCT 07-NOV-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4027
            SAMEA114569841-rid20112183.denovo.050, whole genome shotgun
            sequence.
ACCESSION   DARBET010000049 DARBET010000000
VERSION     DARBET010000049.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA114569841
            Sequence Read Archive: ERR12203666
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 19374)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 19374)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001972618.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001972618.1
            Genome Coverage       :: 132x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/07/2023 13:18:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,041
            CDSs (total)                      :: 2,975
            Genes (coding)                    :: 2,908
            CDSs (with protein)               :: 2,908
            Genes (RNA)                       :: 66
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 67
            CDSs (without protein)            :: 67
            Pseudo Genes (ambiguous residues) :: 0 of 67
            Pseudo Genes (frameshifted)       :: 26 of 67
            Pseudo Genes (incomplete)         :: 28 of 67
            Pseudo Genes (internal stop)      :: 20 of 67
            Pseudo Genes (multiple problems)  :: 6 of 67
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19374
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA114569841-rid20112183.denovo.050"
                     /isolate="VRE4027"
                     /db_xref="taxon:1352"
                     /country="Denmark"
     gene            complement(645..1148)
                     /locus_tag="SCJ86_001886"
     CDS             complement(645..1148)
                     /locus_tag="SCJ86_001886"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332027.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VanZ family protein"
                     /protein_id="HEG1169424.1"
                     /translation="MRYVSKFLLGIYLFFLLWLILFKLSFNLPQFLTYSYSNVNLVPF
                     STFSFENTTVLRETTYNLIVFFPFGVLLNVNFKRLSFSKKLGIIFLVSFLAELIQFLF
                     GIGVADITDLITNTTGGLIGLWAYQLLNKHLSTNKLDRLAIILGYILFLFLIVAFLFL
                     TRTVRLQ"
     gene            1507..2532
                     /locus_tag="SCJ86_001887"
     CDS             1507..2532
                     /locus_tag="SCJ86_001887"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288649.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FUSC family protein"
                     /protein_id="HEG1169425.1"
                     /translation="MFKTYFRFNKIQDNPFRIMNLMLLIFIVLWLGYINHDFLISSFS
                     SLGIFTFFYYQNIPMKYLLKRMFLIGCGLLVAFVLGILSTYVLWLEPFAVALVAFSSR
                     FVLRLFHISKPGGLFFAMLSAMGTSMQLPIAQLPIVSLYFFMGVVFALIAAVITKLLD
                     SRPEQTIEKATLKERFHEEPLVIIDSVFYSAALFLSVYVSHGLNLHNPYWLTLSCASI
                     LLAENLDAMKHRQVQYLIGSMGGLCVSAFLSFVPFTQLQTIFLITFLYGIAQFLVARN
                     YAVANIFLNPMALMLSTLIRGAYLISLIEYRFLGIVIGSFIGLGVAWVMTVGLQHYLT
                     VVRQKLE"
     gene            2855..3733
                     /locus_tag="SCJ86_001888"
     CDS             2855..3733
                     /locus_tag="SCJ86_001888"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016627881.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5596 domain-containing protein"
                     /protein_id="HEG1169426.1"
                     /translation="MRFKDMKRGNLMKKISNEIQILAETLTLPKVVIEQMIVQAKNIL
                     DEVFEQYSHELMDPNKAKATAKKIDKVYEEEPFAALAIYLYAASQSWKKIYQPLKIPR
                     TIYLATMNAFTRFIHEHFQVTGSYRFDRGFWIWHYTSGLIFRIGELEYEMTYFPKGYT
                     DLRLEGKQCLSLHIPSDADLSQNKIRDSYESAIRFFELYFPDYHYQVMYTDTWLLSPN
                     LTKWLKKESKICLFAADYRLLSVDEQDDSGVPWIFGRVDAQIHDYPESTSLQRQAKEQ
                     LLAGEHIGSGLGILTS"
     gene            4209..4325
                     /locus_tag="SCJ86_001889"
     CDS             4209..4325
                     /locus_tag="SCJ86_001889"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002383181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative metal homeostasis protein"
                     /protein_id="HEG1169427.1"
                     /translation="MEKTDISSAYRRLKSPNIKTRKRALKIIKDVKRNSGKR"
     gene            5253..5591
                     /locus_tag="SCJ86_001890"
     CDS             5253..5591
                     /locus_tag="SCJ86_001890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294334.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEG1169428.1"
                     /translation="MTQPVSSEEHDNLFRATEEFKKYQENIVPETPLASEPITDIDGN
                     QIGWYIQYEVMVVNNDSIVKNTVGLELTPIEKVSSLLTYIYTDESDETEIILTDYSKI
                     ATDKKYILWM"
     gene            5685..5915
                     /locus_tag="SCJ86_001891"
     CDS             5685..5915
                     /locus_tag="SCJ86_001891"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008267257.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEG1169429.1"
                     /translation="MLVEKGKENIYYVNVAKVREDENEWKEFKSRYSINSTPTFTVYR
                     EGSIEKTVFWTKESGMSLAEVEEFLDYVSMQQ"
     gene            6315..6686
                     /locus_tag="SCJ86_001892"
     CDS             6315..6686
                     /locus_tag="SCJ86_001892"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cro/Cl family transcriptional regulator"
                     /protein_id="HEG1169430.1"
                     /translation="MRDYVTDKTQEVINQSQSLNELSDKAKEEAVAIISSGISDYIDM
                     SGEINYGDKDKIVRMIKAFIKTFDVKADLKFNDTYLVGKLINILGTEKDTETFISSLS
                     MLFTDKHFSTQLGGEELVEIF"
     gene            complement(6960..7652)
                     /locus_tag="SCJ86_001893"
     CDS             complement(6960..7652)
                     /locus_tag="SCJ86_001893"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288638.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family phosphatase"
                     /protein_id="HEG1169431.1"
                     /translation="MKGIIFDFNGTMFLDSHLHEAAWLHMIHDHTKDGLSDEDILKNI
                     HGRTNTEILKHFISEHLTASEIAVLSEEKESYYRMLCLQNENELQLTKGLEATLDQLT
                     DQQIPLTIATATVKENVAFYFDIFDLARWFDWDKIVYDDGSFPGKPQPDIFLKAADKL
                     SLAPADCLVIEDAYSGLLAAKRAGIGTIIAIDPFGKNKTVFEQAQLNKDGTIKDFTGF
                     WTEHLSATSVNS"
     gene            complement(7655..8440)
                     /locus_tag="SCJ86_001894"
     CDS             complement(7655..8440)
                     /locus_tag="SCJ86_001894"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738601.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR/GlpR transcriptional regulator"
                     /protein_id="HEG1169432.1"
                     /translation="MLKKERLLTIVEMVNKKGILTVNEIINQLDVSDMTVRRDLDELE
                     KSGKLLRVHGGAQSLSYTLDQELSHTEKATLQIQEKKEIAATAASLIHEGETIFLGPG
                     TTIELLATHLLNKKIRIITNNYPVFDILAQADGPEILLIGGDFRKNTGAFVGPITNDN
                     LRRLKFTKAFISSNGVHNEAISTYSTEEGEAQQIALNNSRKKYLLVDNKKFNRDDFYV
                     FYNLHDFDLLITDRQISREVQEHYAQYVDLQIADSTENTTIGA"
     gene            8677..10077
                     /gene="lacG"
                     /locus_tag="SCJ86_001895"
     CDS             8677..10077
                     /gene="lacG"
                     /locus_tag="SCJ86_001895"
                     /EC_number="3.2.1.85"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723883.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-phospho-beta-galactosidase"
                     /protein_id="HEG1169433.1"
                     /translation="MLKLPEDFIFGGATAAYQVEGATKEGGKGAVAWDDFLEEQGRFS
                     PDPASDFYHQYAKDIELCERFGVNGLRLSIAWSRIFPDGAGKPNPEGIAFYHRVFEEC
                     KKRNVTPFVTLHHFDTPKRLFDHGDFLNRETIEAFVSYAIFCFHEFKEVKVWSTFNEI
                     YPVATNQYLLGVFPPGIKYDFTKIVACLHNMMVAHARVVNYFKENELPGEIGVVHSLE
                     TKYAATDAPEDKHAAFLDDALSIRFLLDATYLGYYSTETLTALDEICEANQASYHFPE
                     EDFVELKKASTRNDYLGINHYQCHFVKAYDGENAIHHNGTGEKGTSVYKVKGIGERIY
                     KEGIPRTDWDWLIYPEGLYDLLLRIKSDYPHYNKIYITENGMGYKDQFEDGIIMDQPR
                     IDYLRVYLESLSKAITAGVNVKGYFLWSLMDLFSWTNGYNKRYGLFYVDFETQKRYPK
                     ESAYWYKLVSETKTII"
     gene            10102..10419
                     /locus_tag="SCJ86_001896"
     CDS             10102..10419
                     /locus_tag="SCJ86_001896"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010749967.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS lactose/cellobiose transporter subunit IIA"
                     /protein_id="HEG1169434.1"
                     /translation="MNKEELQMLGFEIVAYSGDARSTLLKLLKEVRQGNFEHVDSALK
                     EADENLTLAHNSQTKILAEEASGKEMEMGFIFIHGQDHLMTTLLLRDLIQDFIVLYRQ
                     NQG"
     gene            10432..12105
                     /locus_tag="SCJ86_001897"
     CDS             10432..12105
                     /locus_tag="SCJ86_001897"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS lactose transporter subunit IIBC"
                     /protein_id="HEG1169435.1"
                     /translation="MDGIIKQIEKGKPFFEKISRNSYLGAVRDGFLTAMPAILFSSIF
                     ILIAAVPEIFGYQWPESVSTWLWKVYNYSMGVVGLLVSATTARCLADSMNRRLPKNKV
                     INTTSVMLASVVSFLLLSVSPIEGGFSTEYMGTKGLLASFVAAFLTVNLYKFCVLKDI
                     TIKMPKEVPGTISQTFRDIFPFSFSVFAAVLIDLGIRQIFQISFAEAIITVLQPLFTA
                     ADGYLGIAIIWGAMALFWFVGVHGPSIVEPAIAAIIYANVETNLQLFNSGEQASKVLT
                     VGLGNFVGTMGGTGATLVVPFIFLLFAKSKQLKAVGKASFIPVSFAVNEPLLFAAPMI
                     LNPYFFIPFLLTPVVNVCLFKFFVDVLGMNSFMYVLPWATPAPIGLVLGTGMGILAFV
                     LAAVLIVVDFLIYLPFCKAYDAVLVEKEAQKAEVIATEEGASADVREEVPVAASQVIS
                     DANKEINVLVLCAGAGTSAMLANALKKGASESKAPISAMAGAYGSHYEMLQDFDMVVL
                     APQVNSYYDDIKEDTDRLGIKLVATKGAEYIGLTRDPEKAVQFILNQFQ"
     gene            12119..13750
                     /locus_tag="SCJ86_001898"
     CDS             12119..13750
                     /locus_tag="SCJ86_001898"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyl hydrolase 53 family protein"
                     /protein_id="HEG1169436.1"
                     /translation="MKMGIWLRKAVLFAAVLGALGAVSKTVEASASEFIRGADISILS
                     DMEKSGAKYYEDDISKDALEILKNNGVNYVRLRLWQDPYNSNGESYGAGTNDLETTID
                     LAKRAKNLGLKVLLDFHYSDFWVDPGKQNLPKAWQGLTFEEMNTALYDYTKNVLSEMK
                     QLDVYPDMVQIGNELNSGMLWPYGKSWGEGGGEFDRLAAFLKSGIQAVRDTQPKTTPV
                     MLHLADGGDTGAFTWWFDEITSRGVSFDLIGVSYYPYWHGSMNELQTNMNAISQRYGK
                     EVIVVETAYGHTTANADTMPNAFGEAEAAAGGYEPTPTGQAEYLLDLADRIQAVPNNR
                     GAGFFYWEPLWYNGNVSWATQAGMNYLSVQSTMGNEWDNQAMFDFAGNALPSLRAFKQ
                     AGAQINLVKNASFESDGPTTSPSNWQTWFQQGTAKGTIKTEAGTFFGDYKLTFWQDQA
                     YEASAYQNVSGISKGTYTLSAYVMSGGEFDTNQLYIKNYGGAELNTAVVKTSEWTKIT
                     IKDIPITTGVCEIGVYSKASGNAWTNVDFVSLVKQ"
     gene            14622..15812
                     /locus_tag="SCJ86_001899"
     CDS             14622..15812
                     /locus_tag="SCJ86_001899"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000997693.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS256-like element ISLgar5 family transposase"
                     /protein_id="HEG1169437.1"
                     /translation="MNDFTTEILKTLANKGDLNELFRVHLEKAVNTLLKTELTAFLDY
                     EKYDRIGFNTGNSRNGSYDRTVKTEYGELHLQIPRDRNGEFKQQTVPAYRRTNDTLEE
                     TVIHLFRKGITMSEIADLIEKMYGHYYTPQTMSNITKSFTEEVTAFNGRELHDRYAAI
                     YMDATYIPLKRKTVAKEAIHIAVGIRPDGSKEVLSYAIAPTESITIWEEILLDLQERG
                     LKNVLLFITDGLKGMVGAISRFYPKARFQHCCVHVSRNISHKVRVDDRKEVCDDFKMV
                     YQASSKEVALEARGTFAEKWKTSYPKVVESILSNDHLLTFYDFPLAIRKSIYSTNLIE
                     SFNKQIKKYSHRKEQFQNEESMERFLVSSFDTYNQKFLGRSHKGFQQAEGELEQMLSQ
                     LIEN"
     gene            15946..18135
                     /locus_tag="SCJ86_001900"
     CDS             15946..18135
                     /locus_tag="SCJ86_001900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000470930.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HEG1169438.1"
                     /translation="MFISTLQIRNFRNYESEIFRFDNETNTIIGENDSGKTNALTALR
                     ILLDDSYYYSSKTLKESDFFHGIQNGWQGHWIIISATFEGISEEEFDNEICASISLNS
                     ESQTILEELISNADKGVGSISLFIRPNKAIRKQLFDISTELDSHQFNEFRNSIRLSDY
                     EFYYTSKSNLNFCINENYDKLVGRLNDNIASNPEEDDEALLGSRIDMSDIFKHISVVY
                     VDALRDVLREMKNNRNPVKRIMETIESKISSDNVESLKTIIQQLNETITSVPEIRKIG
                     ENINRQLNSIIGSVYSPNLLLSSTMSDDMGSLAKFLSMKPEQNIDLDLLGLGHLNMIY
                     LALKIVEFEACRSRELLNIMLIEEPEAHIHHHIQKTLFEGLNLQKNYTQILMTTHSVH
                     LAEVSEISRMNVLKSFNGKSIVMNPANNLNSFAKEKLKKNNLNLINAVERYLDVKRNG
                     LLFSKGVILVEGDAEEILIPQIVKKLFGINLDELGIGLINVGSTSFEYIASLFDDDRI
                     QRKCAIVTDLDEQAIPSEHRLYKSTAQEKGQARKEKLSSLFNENNWVNCFYANTTFEI
                     EFLDVNKENILTYVSPIIDDAFKDKKTITAYKEEIQSEQYLERNLAMLKLANHVGKGW
                     FAIELGEKIDFLIKIPKYILNAIAFACQEIVDLSIYKKILLHTIDLYEETTELSALSA
                     SLKTSDTADLNIAIISLLEYFGKDEDVVYLINQIENYTKILYAEVSK"
     gene            18132..>19374
                     /locus_tag="SCJ86_001901"
     CDS             18132..>19374
                     /locus_tag="SCJ86_001901"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000181735.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent helicase"
                     /protein_id="HEG1169439.1"
                     /translation="MTNIFEKIVNDLNLEQKDVVESEDNLYVNACPGSGKTRVLTRKI
                     AYQAIKHDGSLKRIIAITYTNRAAEEIKERLSLLSIDDDINIWVGTIHQFCLEFIIYP
                     FKMNLSRVSKGFTIIDDYTQRAYVSQINNMLNLNLKTYELNKIDLSLSHDFKINEVQY
                     TQVSEMYHTMLEKNKEIDFDMILLVAYKILTVNSNVARNIASNIRAIYVDEFQDTREL
                     QYNVIAKLLQNNPQIQSMFVGDIDQAIYGSLDGIAKSVEELEMLTSHTFQSKTLHGCY
                     RSNQRLVDFYSNFQSCPYEIESRGNNKNDRGFISYDCKVTKEDLPNIIKNIIKEKIES
                     GIHEKDICVLAPQHYPLFSLGEYLKKELPYCNFDAINISPIKVNNHSLFFKLAILYFT
                     ESGEKVRRRKLIANDILIILIV"
BASE COUNT         6383 a         3086 c         3825 g         6080 t
ORIGIN      
        1 ataaaaaatt agtcaaagaa gggcgtatag atttcttttg ataggagata tacaacagaa
       61 atgaataaat attaacgtgt tttcaaacag tataaaacgg tgtggttttt ggaataaaaa
      121 aataacatat ctcattttac gcttttgtta ttttatactt aaaaaataac gacacagaaa
      181 ataccgtgtc tcagatttct ctactacttc taaaaactac tgtttttttc ctttgaaatg
      241 ccttcaaaaa gtaggatcag gcagaatata ctggcagaat caaggagaag ggagcctaaa
      301 taaggagaaa aaccatagaa aatataaagt aattacttat aaaacaagct ttttatagaa
      361 aaataatata cctgagtagg gtataaatta agacggaatt cctttctaga ttgagatatt
      421 gactaggaag aatgttaaga ttttaagcct ttaagctaaa aaaaaaccct ttttgttaaa
      481 taaggggaat atagcctgtc agtttcaaac tgcaagtcgt cctgctgcaa taatatagta
      541 gattagagat gaaaatagat ttttttaaac caatgactga ataaaaagac cgtcatagat
      601 acacattgga atgtgtctat tacggtcttt ttttacgaac gattttattg aagacggaca
      661 gttcgggtca aaaataaaaa agctacgatc agaaataaga ataaaatgta tcccaaaata
      721 atggctagac ggtctaactt atttgtcgat agatgtttgt ttagaagttg ataggcccat
      781 aaacctatca gtcctcctgt tgtattagtt atcaaatcag tgatgtccgc tacgccaata
      841 ccgaatagga attgaatcaa ttctgccaag aagcttacaa ggaaaataat ccctaatttt
      901 ttagaaaaag aaagtctctt gaaattgaca ttcaaaagca ccccaaaagg aaaaaagaca
      961 atcaagttat aagtggtctc acgtaatacc gtcgtatttt caaaagaaaa agtagaaaaa
     1021 gggacgagat tgacattgct gtaagaataa gtgagaaatt gtggaagatt aaatgatagc
     1081 ttgaataaaa tcaaccataa tagaaagaat aaataaatcc ctaataaaaa ttttgataca
     1141 taccgcatag acatctcctc catttaaaaa gtataccatc gaaaaccgct gaaaaatcat
     1201 aaaaaagtat gaagtttaag gaccaacgta gttatcgtac gaaaattaga agcttttttt
     1261 tgttgggaat ccggtttctt taacaaagct aagatagcga gttgcttgga aaagttgcta
     1321 tcttttttct gtcaaaacga ctcggaatgt tcgttttttg ttctaatttc tccaagttgt
     1381 ttactttttg atactaagtt tatgaaaaat caagaaatag ttgttatact aggtaatgct
     1441 gacaaacggg attggcattt catcttggtt cagctttttt tccaattact aggaaggagg
     1501 gatagattgt tcaagactta ttttcgtttc aataaaatac aagataatcc ttttcgtatc
     1561 atgaatctga tgctacttat ctttattgta ttatggttag gttacataaa tcatgatttt
     1621 ttgatttcaa gtttttcatc attagggatc tttacttttt tctattatca aaacataccg
     1681 atgaaatatt tgctgaaacg tatgtttctg atcggttgcg gactgttggt tgctttcgtt
     1741 ttggggatct tatcgactta tgttttatgg cttgaaccat ttgccgtagc tttggttgct
     1801 ttttccagca gattcgtgtt gcgattattc catatttcta aaccaggtgg cttgtttttt
     1861 gcgatgcttt ctgccatggg aacgagcatg cagctgccaa tcgctcaatt accgatcgtc
     1921 tcactttatt tttttatggg tgtcgttttt gccctgattg cggcagtgat tacaaaatta
     1981 ctggattcac ggcctgagca gacaattgaa aaagcaactt taaaagaaag atttcacgaa
     2041 gagcctttag tgatcattga ttctgttttc tacagcgctg ccttatttct ttccgtttat
     2101 gtcagtcacg gcttgaatct gcataatcct tattggttga ccttgtcatg tgcatcgatt
     2161 ttgctagcag aaaacttgga tgcgatgaag catcgacaag tccaatatct gattggatcg
     2221 atgggtgggt tatgtgtgtc ggcattcttg tcatttgtgc cgtttacgca gttgcagacc
     2281 atttttctga tcacttttct atatggaatc gcacaatttc tagttgctag aaattatgca
     2341 gttgctaata tttttcttaa tccgatggcg ttgatgctat ctacacttat tcgtggagca
     2401 tacttgattt ctttgatcga atatcgtttt ttagggatcg tcataggaag tttcatcggt
     2461 ttaggtgttg cgtgggtaat gacggttggt ttgcaacatt atttaacggt tgttcgtcaa
     2521 aaattagaat agaaggacaa atatatgtgc caaaagaggt tatgacaaga tttttgtcac
     2581 aacctctaat tcaaggtgtt caaaagccag attctcagca ttaagtcaaa ttcacacaaa
     2641 aatgtggaaa ggaactttca aaaaaatttt atatttattc tatcaaaaaa ttttgtttat
     2701 gatggtaact gaataaactt tcctgagatg gtcaatgctt tccttgaaag tcttacagat
     2761 attctttctt catagaaagt ttaagtttta tagttagtgt acaataaaag tatgtttata
     2821 ccatatggtg tccggataat cagtcattta aacaatgagg ttcaaagata tgaaaagggg
     2881 aaatttgatg aagaaaataa gcaatgaaat ccaaattctg gcagaaactt taactcttcc
     2941 caaagtcgtt atagagcaaa tgattgtaca agcaaaaaac atccttgatg aggtgtttga
     3001 gcaatatagt catgaactaa tggatcctaa taaagcaaag gcgactgcaa agaaaataga
     3061 caaagtctat gaagaggaac cttttgcagc attagctatt tatctatatg cagcttctca
     3121 aagttggaaa aaaatttatc aaccattgaa gattccaaga actatctatt tggcaacaat
     3181 gaatgctttt actagattta ttcatgaaca tttccaagta acaggaagct atcgctttga
     3241 ccgtggtttt tggatttggc attacactag tggcttgatc tttcggattg gtgaattgga
     3301 gtacgaaatg acatattttc cgaaagggta cacggatcta agattagaag gaaagcagtg
     3361 cctttctctt catattcctt ccgatgcaga tttatctcag aataagataa gagatagtta
     3421 cgaaagtgcc attcgttttt ttgaacttta ttttcctgat taccattacc aagtgatgta
     3481 cacagatact tggttactat cacccaatct tactaaatgg ttgaaaaaag agtcaaaaat
     3541 ctgtcttttt gcagccgatt atcgattatt atcagttgat gaacaggacg atagtggagt
     3601 cccttggatc tttggacgag tagatgccca aatacatgat tatccagaaa gtacgagttt
     3661 acagcgacaa gcaaaagagc aattgcttgc aggcgagcac attggctctg gcttaggtat
     3721 tcttacttca taagtttagt acttcgaaat agtttaaaag agcgtgagac aaaagtaaag
     3781 aacacttttg tctcacgctc taaacacgta taaacggcgg aagcagaagc aactccttcg
     3841 gaaataagct gaaattcaca aaaatttgaa aagcaatttt cgtgaatttc ttcttatttc
     3901 ttggagttaa acgcttctgt cccagcctct tttttagtgt tctatcgaac acacagtacg
     3961 ttcaataggt aaactagcta taacaataga tattaaagtt aacggacaag agggcctttt
     4021 ttaggtagct aaaacgtaat aatgaaaatt tatttccaga gataggagtc aatcagctag
     4081 tctgatcgac tgttttaaca agttgacttt cattttaact tttgatacca tttaaaacac
     4141 aaatcgtaat tgtttcgatt tgtgtttttt agcatgacta caatcaaaga agaaagtggg
     4201 tcgtccatat ggaaaaaaca gatatctcaa gtgcataccg ccgcttgaaa agcccaaata
     4261 tcaaaacacg aaaacgtgcg ctgaagatca tcaaagatgt gaaacggaat agtgggaaaa
     4321 ggtaagaaga aggaaactta attaaaaaaa tagccgaaaa acgtttttcg gctattttca
     4381 ataatcggtt tttgtgtaag gtttccgaaa gaactgctgt tgattttcca tttttaacat
     4441 atgataaatt ggtagaatct ttcttttcaa attgatacca ttccctaatt ccagttatat
     4501 agttaactac agaaccacaa agcacgtacg tttataaaag tattatctgt agttattttt
     4561 caattccctt tcaacctctt attttgacca gtagtattga tcactttacc ttttaacacc
     4621 tttgttcgtt tttgaaacat ttttagtcaa gaatggaaac gtaactacat aatcaaaggt
     4681 tttgcgaaat atttcatgaa atatttcatg tagaagcgag caatacggtt gcaaataacc
     4741 attatcgttt aataagcagt tacgaaagct cccaatatct ctaacgttca gctcactcca
     4801 tttcttttct tcaaaaaaat cagtgattcg tatctctttc tatttgtcga aaaagttaac
     4861 tttaaaaagt aacgccgaaa aaatatgcaa aaaaataaaa aagcagttac tattgctaga
     4921 ataacatcaa ggaataataa gttttggttt tcagataata gtcgataagt tgtgtattgg
     4981 aattttagat acagtaataa agtatttttc atgaaatttt gtgagaatca ctattacaag
     5041 tatctttaat aaaattaatg gttaaggagg aacttaaagt agaatgtaaa tgaatagttg
     5101 tagtcagtcg attgtgatac tagactcaaa gttgcaggaa taagtttttc aatttgtgat
     5161 tggaagataa aaaatgaaaa agaagggctt ttttttatac tttgttgatt gtagtgttta
     5221 ttttccctct gtttagccaa gtaacatttt ctatgactca accagtttca agtgaggaac
     5281 atgataacct ttttcgtgct acagaagagt ttaagaaata tcaggaaaat attgttccgg
     5341 agactccatt agctagtgag ccaattactg atattgatgg aaaccaaata ggatggtata
     5401 tacagtacga agttatggtg gtaaataatg actcaatagt aaaaaataca gtaggcttag
     5461 agctaacacc gattgaaaag gtgagttcat tgttaacgta tatttatact gatgaaagtg
     5521 acgagactga gatcatttta acggattact caaaaattgc tactgataaa aagtatatat
     5581 tgtggatgta gacactagtg aaatacttta tgatatagaa attgatgaaa atttactaga
     5641 tgtagcacaa gaaatcgatc atcaaaagca ggaagctatt cgatatgctg gtagaaaagg
     5701 gaaaagaaaa tatttattac gtgaatgtag caaaagttag agaagatgaa aatgagtgga
     5761 aagaattcaa gagtcgctac tcaattaact cgactccgac atttacagtt tacagagaag
     5821 gatcgataga aaaaactgtt ttttggacta aagaaagtgg aatgtcttta gctgaagtag
     5881 aagagttttt ggattatgtt agtatgcaac agtaagggag ttcttattat gaaaaatttt
     5941 gacacatatt atgttattgt tttttcgata tctttgctgt taatattact aactttaatg
     6001 cagtataaag agtatccaga gtattttatt ttaacttttg tatatttagt aagtagctac
     6061 acacttctat ataaattcag aagttcttct agagtttcta aagtttgttt cttaattagc
     6121 ttattgattc tataatattt ttgatttata taggaaatta aaaatattat agaatcaaca
     6181 ctcagagaac gggttggttt ttaatagatt taataagtac tgataatgaa atttctcctg
     6241 gttgggaaaa cgaagttggt taccctgtag aagaattttt tcaagattac tttgcggagt
     6301 atgccgatag gataatgaga gattacgtaa cagataaaac tcaagaagtc atcaatcaat
     6361 cccagtcatt aaatgaatta tctgataagg cgaaagaaga agcagttgcc attatttctt
     6421 cagggattag tgactatatt gatatgagtg gtgaaatcaa ttatggcgat aaagacaaaa
     6481 ttgtacgcat gataaaagct tttatcaaaa cttttgatgt gaaggcagat cttaagttta
     6541 atgatactta cctagtagga aaattaatta atatccttgg gacagagaaa gatacggaaa
     6601 cgtttatttc ctctttgtct atgttgttta cagataagca tttttcaact cagcttggtg
     6661 gagaggaact agtggaaatc ttttaaccat tacgcccagc attaataaaa gtatatcaaa
     6721 aaagtactcc tgatgatttt taagattggt ttgagaaata atgtgaaaac actattttta
     6781 atgttaatta tagatagaga tgaacgcgtc atatggttaa aagaatgaaa aagtcaaaga
     6841 tacactgcta tatttcacta aggatatagc agtgtatctt tcatgtcgtt tcactggcag
     6901 ttttttataa aaggaggtgt gcatttcagt tgcttttctt tggtagttag ttgaatcgtt
     6961 taagaattaa cggatgtggc agacaggtgc tctgtccaaa aaccagtaaa atctttgatg
     7021 gttccatctt tatttagctg tgcctgttca aatactgttt tgtttttacc gaatggatca
     7081 attgcaataa ttgttccgat ccccgcacgt ttggcagcaa gtaatcctga gtaagcatct
     7141 tcgataacaa gacaatcagc cggagctaag ctcagtttgt ctgccgcttt aagaaaaata
     7201 tccggttgcg gtttcccagg gaaagagccg tcgtcataga caatcttgtc ccagtcaaac
     7261 cagcgggcta aatcgaaaat atcaaaataa aaagcaacat tctctttgac agtagccgtg
     7321 gcaatcgtta atggaatctg ttggtcagtc agctggtcaa gagtcgcttc aagccctttt
     7381 gtcaactgaa gctcgttttc attttgcaag caaagcatac gataatagct ttctttttct
     7441 tctgacaata cggcgatctc agaggcggtc aaatgctctg aaatgaaatg tttcaaaatc
     7501 tccgtattcg tccgaccatg aatatttttt aaaatatcct cgtctgataa accatctttt
     7561 gtatgatcat gtatcatatg cagccaagca gcttcatgca gatgagagtc caaaaacatt
     7621 gttccgttga aatcaaatat aatccctttc atagttacgc tcctatagtg gtattttcgg
     7681 ttgaatcagc aatttgcaga tcgacatatt gtgcatagtg ctcttgaacc tcccgcgaga
     7741 tctggcgatc cgtaatgagc agatcgaaat catgcaaatt ataaaacaca taaaaatcat
     7801 ctcgattgaa ttttttatta tcaacgagta gatatttctt acgggaatta tttagagcga
     7861 tctgctgtgc ttctccttct tcggtactgt aagtagagat cgcttcgttg tgaacgccat
     7921 ttgaactgat aaatgctttt gtaaacttta gtcttcttaa attgtcgttg gtgatcggtc
     7981 cgacaaatgc gccggtattt ttccggaaat caccgccaat cagtagaatt tctggtccat
     8041 ctgcttgagc aagaatatca aaaactggat aattatttgt aatgatccga attttcttgt
     8101 tcagtaaatg cgtcgccaat agttcgattg ttgtacctgg tcctaagaaa atcgtttctc
     8161 cctcgtgaat caaggaggct gctgtggcag caatttcttt tttttcttga atctgcagag
     8221 tggctttttc agtgtgagaa agttcttgat ctaaagtgta ggaaagactt tgagcccctc
     8281 catggacacg cagcagcttg cccgattttt ctaattcatc taaatctctg cgaacggtca
     8341 tatcagaaac atctaattga ttgatgattt cattaacggt aaggattcct tttttattga
     8401 ccatttctac gatagtaagc aatctttctt ttttcaacat tgtcttcacc tctaatttaa
     8461 ggataatcta tcatataaac ggacaacagt caatctaatt cgaacaaaag taaacattta
     8521 gggtggtttt tatgtttgtt acaatagatt ttattgctgt tttctggttt ttatgattat
     8581 ttgtgtttgt ttttgttgac aaatgttttg gatgcgttta caatgcaatc agttagaaag
     8641 agcgaaaact gattatagat ttgaggagag atgtacatgc ttaaattacc agaagatttt
     8701 atttttggcg gagccacagc agcctatcag gttgaaggag ccactaagga aggtggcaaa
     8761 ggcgctgttg cttgggatga ctttttagag gaacaagggc gattcagtcc cgatccggca
     8821 agtgattttt atcatcaata tgcaaaggac attgaattgt gtgaacgttt tggtgtaaat
     8881 ggtctaagat tatccattgc ttggagccgc atttttcctg atggagctgg gaaaccaaat
     8941 ccagaaggaa tcgcttttta tcatcgtgtg ttcgaagaat gtaagaaaag aaacgtaacg
     9001 ccgtttgtga cgttgcatca ttttgatacc cctaaacgtt tgtttgacca tggagatttt
     9061 ttgaatcgag aaacaataga agcatttgtt tcctatgcca ttttttgttt tcatgaattc
     9121 aaagaagtca aggtgtggag tacctttaat gaaatctatc cagttgcaac caatcaatat
     9181 ttgttaggtg ttttcccacc aggaatcaaa tatgatttta caaagatcgt cgcctgtctt
     9241 cataatatga tggttgctca tgcccgtgta gtgaactatt tcaaagaaaa cgaacttccg
     9301 ggtgaaattg gtgtagttca ctcattagag acgaaatatg cggcaacaga cgcgccggaa
     9361 gataagcatg ctgccttttt agacgatgca ttgtcgatcc gtttcttatt agatgctacc
     9421 tatttaggct attattcaac tgaaaccttg actgctcttg atgagatatg tgaagcaaat
     9481 caggccagct atcattttcc tgaagaagat ttcgttgaat tgaaaaaagc atccacgcgt
     9541 aatgactact tagggatcaa tcactaccaa tgccattttg tcaaagctta cgatggagaa
     9601 aatgcaatcc atcataacgg tacgggtgaa aaagggacct ctgtttataa agtgaaggga
     9661 atcggagaac gtatttataa agaaggaatt cctcgaactg attgggactg gctgatttat
     9721 ccagaaggtc tatatgactt gttgctgcga attaaaagcg attacccaca ttacaacaaa
     9781 atctatatca ctgagaacgg gatggggtat aaagatcagt ttgaagatgg cattattatg
     9841 gatcagccga gaatcgacta tttgagagtc tatttggaat ccttaagcaa ggcgataaca
     9901 gccggggtaa atgtgaaggg gtattttttg tggtcgctta tggatctttt ctcatggaca
     9961 aatgggtata acaaaaggta tggcttgttt tatgttgatt ttgaaaccca gaagcgttac
    10021 ccaaaagaat cagcttactg gtacaagttg gtcagtgaaa ccaaaacgat tatttaataa
    10081 gtgataatgg aggaagaaaa gatgaataaa gaggaattac aaatgcttgg ttttgagatc
    10141 gttgcatatt caggcgacgc tcgcagtaca ttattgaaat tattgaaaga agtaagacaa
    10201 ggaaactttg aacatgttga ttcggcgttg aaagaagccg acgaaaattt aacccttgca
    10261 cataattcgc aaacaaaaat tttagcggaa gaagcatctg ggaaagagat ggagatggga
    10321 ttcattttta tccatgggca ggatcattta atgaccactt tattgctgag agatctgatt
    10381 caagacttta ttgtactgta cagacagaat caaggatagg agaagaacgc tatggatgga
    10441 atcattaaac aaatcgaaaa agggaaacca ttttttgaaa aaatctcacg taatagttat
    10501 ttaggagccg ttcgtgacgg atttttaaca gcgatgccag ctattttatt ttcaagcatt
    10561 ttcattttga ttgctgcggt tcctgagatt tttggttacc aatggcctga atctgtctcc
    10621 acatggctat ggaaagtcta taattattcg atgggtgttg tagggttact cgtttcagca
    10681 accactgctc gctgcttggc tgattcgatg aatcgtcgct taccgaaaaa caaagtaatc
    10741 aacaccacct ctgtgatgct ggcctctgtg gtaagtttcc tgttgttaag cgtctctccg
    10801 attgaaggtg gattttcaac ggaatatatg gggacaaagg gactactggc ttcctttgtc
    10861 gctgcatttt taacagttaa tttatataag ttttgtgtgc tgaaagatat taccattaaa
    10921 atgcctaaag aagttcctgg aacgatttcg caaaccttcc gagatatttt tcctttctct
    10981 ttttcagtat ttgcggccgt tttaattgat ttaggcattc gacaaatctt ccaaatctca
    11041 tttgcagaag caatcattac ggtgctgcag ccgctattta cagcagctga cgggtactta
    11101 ggaatcgcta ttatctgggg agcgatggcg ctgttttggt ttgtcggtgt tcatggaccg
    11161 tcgatcgtgg agccggcaat tgcggcaatt atctatgcca atgtggaaac caatctgcaa
    11221 ttgttcaatt ccggtgaaca agcttctaaa gtactgacgg tgggtttagg gaattttgta
    11281 ggaactatgg gaggaactgg ggcaacattg gttgttcctt ttatcttcct actctttgct
    11341 aagtctaagc agttgaaggc agttgggaaa gcttccttta ttccagtaag tttcgctgtc
    11401 aacgaaccat tgctatttgc ggcacccatg attttgaacc cttatttttt tattccattt
    11461 ttgttaacac ctgttgtaaa cgtttgcctg ttcaagtttt ttgtcgatgt attgggcatg
    11521 aacagcttta tgtacgtttt gccttgggca actccagcac cgatcggttt ggttctagga
    11581 acgggcatgg gtatcttagc ttttgttcta gcagcggttc taatcgtcgt tgacttcctg
    11641 atctatctac cattttgcaa agcatacgat gctgtcttag tagagaaaga agcacaaaaa
    11701 gcagaggtga tcgcaaccga ggaaggtgct tctgccgatg tacgtgagga agtacctgtg
    11761 gcagcgagtc aagttatcag cgatgcaaat aaagaaatca atgtgttggt actttgtgct
    11821 ggcgcaggga caagtgcgat gttagctaat gctttaaaaa aaggtgcatc tgaatcgaaa
    11881 gcaccgatca gtgcaatggc gggtgcttat ggttctcatt acgaaatgct gcaggatttt
    11941 gatatggtgg tcttagcacc gcaagtcaat tcttactatg atgatatcaa agaagacacc
    12001 gatcgcttag gaatcaagct ggttgcgaca aaaggagcag aatatatcgg attaacgaga
    12061 gatcctgaaa aagctgttca gtttattctg aaccaatttc aataaaggag gagccataat
    12121 gaaaatggga atttggttgc gtaaagctgt tttatttgca gctgtgttag gggctttggg
    12181 ggctgtttca aaaactgttg aagcatctgc cagcgaattt attcgcggag cagatatttc
    12241 gatcctgtca gatatggaaa aatctggtgc aaaatattat gaagacgata tttcaaaaga
    12301 tgccttagag attttaaaaa ataatggtgt caactatgtt cgactgcgtt tgtggcagga
    12361 tccttataat agtaatggag aaagttatgg agcagggaca aatgatctgg aaacgacgat
    12421 tgaccttgca aaacgagcaa aaaatctggg acttaaggtg ctgctggatt ttcactacag
    12481 tgatttttgg gttgatcctg ggaaacagaa tcttcctaaa gcatggcaag gtctgacctt
    12541 tgaagaaatg aatacggcat tgtacgacta tacaaagaac gttctgtcgg aaatgaagca
    12601 gctggacgtt tatcctgata tggttcagat cggcaatgaa ctgaatagcg gaatgctctg
    12661 gccttatggc aagtcttggg gagaaggcgg gggagaattt gatcgattgg ctgctttttt
    12721 gaagtctggt atccaagctg ttcgagacac tcaacctaaa acaactccag taatgctcca
    12781 cttagctgat ggtggcgata caggagcatt tacttggtgg tttgatgaaa tcacgagtcg
    12841 aggtgtgtca tttgatttaa ttggagtgtc gtattatccg tattggcacg gttcaatgaa
    12901 tgaacttcaa accaatatga atgcgatcag ccaacggtat ggtaaagaag tgatcgtagt
    12961 tgaaacagct tatgggcata caacagcgaa tgccgatact atgccaaatg cttttggtga
    13021 agccgaagca gctgcggggg gatatgaacc aacaccgaca ggacaagcag agtatttgct
    13081 ggatcttgcc gatcggattc aggcagtacc gaataatcgc ggggcgggct tcttctattg
    13141 ggaacctctt tggtacaacg gaaatgtgag ttgggcaaca caagcaggta tgaattatct
    13201 cagtgttcaa tccactatgg gaaacgaatg ggacaaccaa gcgatgtttg attttgctgg
    13261 aaacgccttg ccaagcttgc gtgcgtttaa acaagcaggg gcgcaaataa atctagttaa
    13321 aaatgcaagt ttcgaaagcg atggaccaac gaccagtcct tccaattggc aaacctggtt
    13381 tcaacaagga accgctaagg gtacgatcaa aacggaagcg ggcacttttt ttggggacta
    13441 caaactaact ttttggcagg atcaagcata cgaggcatca gcttatcaaa atgttagtgg
    13501 cataagcaaa ggaacttaca cattgagtgc ctatgtaatg tctgggggag agtttgatac
    13561 taatcagtta tatatcaaaa attatggtgg agcggaacta aatactgcgg ttgttaaaac
    13621 aagtgaatgg acaaaaatta caatcaaaga cattccaatt acaacaggtg tttgtgaaat
    13681 tggggtttat agcaaggctt caggaaatgc ttggacgaac gttgattttg tttcattggt
    13741 gaaacaatag actgcggatc gtttcgattc aagatcggag gttcccttca gccacatcgt
    13801 tagcgtagaa ggcaggaatc gaacctgcac ctcgcctttg ccaaaaataa ccagaaacga
    13861 tttccagtca tttttaccaa aacgagtaaa gcaccagact tcatctacac tttgtattat
    13921 gctaaagaga gttcaacgat ttcgtaagaa gcaatgaaaa aattgcaaaa agttgtgcag
    13981 ataacataaa gtttgtttaa gaaagggtaa ccttagaatt ccgtcgtgaa tatgatttga
    14041 gcacttagat ggatagaaaa aagttctcgg aaaacagttc gtttttcgag aacttctttt
    14101 tatgataaga atgaaacaaa ttaacttata ttatagtctc ttttggtagg gaaccatctt
    14161 atttttaatg aaaatcaagc attttagcta ttgaagaatg ttgatttata gggattcccc
    14221 ctccatatta tgttggctct ttattcccac tctgttttca acgtaaagtg tataaaacag
    14281 aatgcgatac tatcaaggga gttaaaacaa tatacagaac agtaaaacac tataaataaa
    14341 aataaaagtg caataaaagt gcaatttttg cacctaatca tactctgttt atatatgaaa
    14401 actagattct gaatccaata tttggttaac gagaggcgtt cgaatttgaa cgtcttttat
    14461 atattacttt caaataaata tgaggtagaa tactatctat tttcttttta acccaattgt
    14521 tataatggag agtgtaaaat attttgtgta aatagaaaaa aggaagtccc ttctgtagaa
    14581 tagagttacc acaacacatt cacagaagag aggacttccc tatgaacgat tttactacag
    14641 aaattctaaa gactctagcg aacaaaggcg atttgaatga attattccgt gtccatttgg
    14701 agaaagcagt caatacgctt ctcaaaacgg agttaacggc tttcctagat tacgaaaagt
    14761 atgatcgcat tggttttaac acgggtaatt ctcgtaacgg ctcctatgac cgtacggtca
    14821 agaccgagta tggggaactt catctccaga ttccgcgcga ccgcaatggt gagttcaagc
    14881 aacagactgt tcctgcttat agacggacga atgacacgtt agaggagacc gtcattcacc
    14941 tcttccgaaa aggtattacc atgtcggaaa tcgcagactt gattgagaaa atgtatgggc
    15001 attactacac gccccaaacc atgtccaata taacaaaatc atttacagaa gaggtaacgg
    15061 cgtttaatgg gcgggagctt catgaccgtt atgctgctat ttatatggac gcaacgtata
    15121 ttccgttaaa gcggaaaacc gtcgccaagg aagctattca tatcgcagtt ggcattcgcc
    15181 cggacggatc aaaggaagta ttgagctatg cgattgcacc gactgaatcc atcacgattt
    15241 gggaggaaat tttattggac cttcaagagc gcggtttgaa aaatgtcctc ctgttcatca
    15301 cggatggctt aaaggggatg gtaggagcga ttagtcggtt ctatcccaaa gctcgttttc
    15361 aacattgttg tgtacacgtt tcccgtaata tcagtcataa agtgcgtgtc gatgatcgta
    15421 aggaagtctg tgatgatttt aaaatggtgt atcaagcctc atctaaagag gtggcgttgg
    15481 aagcacgtgg tacttttgcg gaaaaatgga aaaccagcta tccaaaagtg gttgaatcga
    15541 ttctttcgaa cgatcacttg ctcactttct atgatttccc cttggccata cgcaagagta
    15601 tttattctac gaacttgatt gaatccttta ataaacaaat caagaaatac agccaccgca
    15661 aggaacagtt ccaaaatgaa gagtcgatgg aacgtttctt agtctcgtct tttgatactt
    15721 acaaccaaaa attcctaggt cgcagtcata aaggtttcca acaggccgaa ggcgagctcg
    15781 aacaaatgct aagccaactg attgagaatt agaaacaatc tgcagtaggg aagaatcatt
    15841 tacacaaaat tattgacgct cccttataat gagaactttt actaacataa tttatgataa
    15901 acttactata taatcatatt aattaaatag ttaggagatc tgcttatgtt tatatctact
    15961 cttcaaattc gaaattttag aaattatgaa tccgaaatct ttcgattcga taatgagaca
    16021 aatactatta taggtgagaa tgattcaggg aaaaccaatg ctcttactgc attgagaatc
    16081 ttattagatg atagttatta ctatagtagt aaaacactaa aagaatcaga tttttttcat
    16141 ggaattcaaa atgggtggca agggcattgg attattatct ctgcgacatt tgaaggaatc
    16201 agcgaagaag aatttgacaa tgagatttgt gcttccattt ctttaaattc tgaaagtcaa
    16261 acaatcttag aagaattgat ttcaaatgca gataaaggtg taggtagtat ttctctattt
    16321 attagaccta acaaagctat tagaaagcaa ttatttgata tttccactga attagactct
    16381 catcaattta acgaatttag aaattctatt cgtctatccg actatgaatt ttactacaca
    16441 tctaaatcaa atctaaattt ctgtatcaat gaaaattatg ataaattggt aggaagactc
    16501 aatgacaata tagctagtaa tcctgaagaa gacgatgaag ctctgttggg ctcaagaatt
    16561 gacatgtcag atattttcaa gcatatttcg gttgtttatg ttgacgctct tcgagatgtt
    16621 cttagagaaa tgaaaaacaa tcgaaaccca gtcaaaagaa tcatggaaac aattgaatca
    16681 aaaatatcat cagataatgt tgagtcatta aaaacaatta tacagcaatt aaatgagact
    16741 attaccagtg ttcctgaaat taggaaaatt ggcgaaaata ttaatcggca actaaatagc
    16801 ataattggtt cagtgtattc tcctaatctt cttctttcct caactatgtc agatgatatg
    16861 ggttctttag caaaatttct ttcaatgaag ccagaacaaa atatcgactt agacttacta
    16921 gggttaggac atctaaatat gatttattta gctttgaaaa tagtcgaatt tgaagcgtgt
    16981 cgttcaagag aactacttaa tattatgttg attgaagaac cagaagctca tattcatcat
    17041 catattcaaa aaactctgtt cgaaggatta aatcttcaaa aaaactatac tcagattctc
    17101 atgaccacac attctgtaca tttagctgaa gtgtctgaaa tatctcggat gaatgtatta
    17161 aaatcgttta atggcaaatc tatcgttatg aatccagcaa ataatctgaa ctcatttgct
    17221 aaagaaaagt taaaaaagaa caatctaaat ctaataaacg cagtagagag atatttagat
    17281 gttaaaagaa atggattgct tttttctaaa ggtgttatct tagttgaagg agatgctgaa
    17341 gaaatactta ttcctcaaat agtaaaaaaa ttatttggta taaacttaga cgaacttgga
    17401 ataggtttga ttaatgttgg tagtacttcg tttgaatata tcgctagctt gtttgacgat
    17461 gatagaatac agagaaagtg tgctattgta actgatttag atgaacaggc aatcccatca
    17521 gaacatcgat tgtataaaag cactgcacaa gagaaaggtc aagcaagaaa agaaaaatta
    17581 tcctcccttt ttaatgaaaa caattgggtt aattgttttt atgctaatac aacatttgaa
    17641 attgagtttt tagatgtgaa taaagaaaat attctaactt atgtcagtcc aattatagat
    17701 gacgcattca aagataaaaa aacaataact gcttataaag aagaaataca aagtgaacaa
    17761 tatttagaaa gaaatttagc gatgttaaaa ctagccaatc atgttggtaa ggggtggttt
    17821 gcaattgagt taggtgaaaa aattgacttt ttaatcaaaa ttcctaagta tattcttaat
    17881 gctattgctt ttgcatgtca ggaaatagta gatctctcaa tttataaaaa aattctactc
    17941 catacaatcg acttgtacga ggaaactaca gaattgtcag ctttatcagc tagtttaaaa
    18001 acaagtgata cagctgattt gaatatagca ataatttctt tattagagta ctttggtaaa
    18061 gatgaggatg ttgtgtatct aatcaatcag atagaaaatt acacaaaaat actttatgct
    18121 gaggtttcaa aatgacaaat atcttcgaaa aaatcgtaaa tgatttaaat ttagaacaaa
    18181 aagatgttgt ggaaagtgaa gataaccttt atgtaaatgc atgtcctggg agtgggaaaa
    18241 caagggtact tacaaggaaa attgcttacc aagcaattaa acacgatgga tcactaaaaa
    18301 gaataattgc tatcacatac actaatagag ctgctgaaga aattaaggaa cgcctaagtt
    18361 tacttagtat agatgatgat attaatattt gggtggggac aatacatcag ttttgtcttg
    18421 aatttattat ttatcctttt aaaatgaatc tctcaagagt atcaaaagga tttacaatta
    18481 ttgatgatta tactcaaaga gcttacgtca gccaaattaa taatatgctg aatttaaatc
    18541 tcaaaacgta tgaactgaac aaaattgatt tgtcactttc tcatgatttt aaaattaatg
    18601 aagttcagta tactcaagtt tctgaaatgt atcacactat gttggaaaaa aataaagaaa
    18661 ttgattttga tatgatttta ctagtagcgt ataaaatttt aactgtaaac agtaacgttg
    18721 cacgtaatat tgctagtaat ataagggcga tttatgtgga tgaatttcaa gatactagag
    18781 aattacaata caatgtaata gctaaattac ttcaaaacaa ccctcaaatt cagtcaatgt
    18841 ttgttggaga tattgaccaa gcaatttatg gatcattaga tggtatagct aaatcagttg
    18901 aagaactaga aatgctaact agtcacactt ttcaatcaaa gacgctacat ggctgttaca
    18961 ggtctaatca gagattagta gacttctact ctaattttca gagttgtcct tacgagattg
    19021 aaagcagagg gaataataaa aacgaccgtg gttttataag ttatgattgc aaagttacta
    19081 aggaggattt acccaacata ataaaaaata taataaagga aaagattgaa agcggcattc
    19141 atgaaaaaga tatttgtgta cttgctcctc aacactatcc tctgttttca ctgggtgagt
    19201 atttaaagaa agaattaccg tattgcaatt ttgatgctat aaatatatca ccaattaagg
    19261 taaataatca tagtttattt ttcaagttag caattttgta ctttactgaa tcaggtgaaa
    19321 aggttcgaag aagaaagcta attgcaaacg atattttaat tatcttaatt gtca
//
LOCUS       DARBET010000050        19126 bp    DNA     linear   BCT 07-NOV-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4027
            SAMEA114569841-rid20112183.denovo.051, whole genome shotgun
            sequence.
ACCESSION   DARBET010000050 DARBET010000000
VERSION     DARBET010000050.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA114569841
            Sequence Read Archive: ERR12203666
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 19126)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 19126)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001972618.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001972618.1
            Genome Coverage       :: 132x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/07/2023 13:18:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,041
            CDSs (total)                      :: 2,975
            Genes (coding)                    :: 2,908
            CDSs (with protein)               :: 2,908
            Genes (RNA)                       :: 66
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 67
            CDSs (without protein)            :: 67
            Pseudo Genes (ambiguous residues) :: 0 of 67
            Pseudo Genes (frameshifted)       :: 26 of 67
            Pseudo Genes (incomplete)         :: 28 of 67
            Pseudo Genes (internal stop)      :: 20 of 67
            Pseudo Genes (multiple problems)  :: 6 of 67
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19126
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA114569841-rid20112183.denovo.051"
                     /isolate="VRE4027"
                     /db_xref="taxon:1352"
                     /country="Denmark"
     gene            complement(<1..703)
                     /locus_tag="SCJ86_001902"
     CDS             complement(<1..703)
                     /locus_tag="SCJ86_001902"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294551.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEG1169440.1"
                     /translation="MFWHFYKYRTKILLRNKSMLFWTLAFPILLGLMFMAAFGEIDQG
                     GNLETINTGIVIKADQTALSENFTTVLEQVEVNDKPLFEVQELSEVEANEKLNADDLA
                     GFYLIDGSSVSLHVGQAGMSQTLMKNVMDQFLQRIGIISSKVAEVENVDLAPIMTVFE
                     QEVSFQEVHAGRNMSFKSFYFFTLIGMAILYGTMWGVRNVQDQQANQSSNGIRLSLIP
                     RKQSLVSIANLAASFT"
     gene            complement(717..1641)
                     /locus_tag="SCJ86_001903"
                     /pseudo
     CDS             complement(717..1641)
                     /locus_tag="SCJ86_001903"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015509966.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
     gene            1758..2837
                     /locus_tag="SCJ86_001904"
     CDS             1758..2837
                     /locus_tag="SCJ86_001904"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327283.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GHKL domain-containing protein"
                     /protein_id="HEG1169441.1"
                     /translation="MHKSRLLNETSFLLVISLLLLVSQGFVAMQVIYLLIAVLFTGVS
                     YLLPTKPNWIFQVILFLLCAFLPDLVLFLPATMRVIAPEESHLDYLFLLGFLLISTVG
                     NHPPIKVAFVVILLFFSFYLRKKDQRTTDLEKKYIVSKDTHWEKQQELSEQNKALALS
                     QETQLALQIVEERNRIARDIHDNVGHLLSSAILQIGALEMINQEPKLKEPLENLSATV
                     HTGMDRIRQSVHDLHETSISFQHSLEFLIADFPSSVTIEGNLFEGLNETQQNCFLMVI
                     KEALTNSIKHSHAKTVVLHFRTLPAFYRLQITNDGTSPQKNSAPGIGLKTMRQRVQEQ
                     KGQVHIFQENQQFQLTIILPREGHQ"
     gene            2869..3462
                     /locus_tag="SCJ86_001905"
     CDS             2869..3462
                     /locus_tag="SCJ86_001905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303134.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HEG1169442.1"
                     /translation="MTKSLQTILESTKEIRVLGIGHCAKDALVLYETHRPDVLLTDIR
                     MPKQTGIDAAKEILAKFPKAILLLLTTFKDEEYIREAFSIGVKGYLIKQNLQAIIPSV
                     KSAYNGQAVFGNEIIETFTQMMKNKPTIDSHAYASFSERELAIIKEVAAGKNNKEIAD
                     ALYLSDGTVRNYISQLLEKLDLRDRTQLAIYFYQHLQ"
     gene            complement(3572..3799)
                     /locus_tag="SCJ86_001906"
     CDS             complement(3572..3799)
                     /locus_tag="SCJ86_001906"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332985.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cold-shock protein"
                     /protein_id="HEG1169443.1"
                     /translation="MAKETEDKLAKLAKESGNDFKKLDEMEKEFKLNESPDVERDDRE
                     ESKLEKTLDKAPIFPPNTPNNPTNPGSPANP"
     gene            complement(3902..6490)
                     /locus_tag="SCJ86_001907"
     CDS             complement(3902..6490)
                     /locus_tag="SCJ86_001907"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent RecD-like DNA helicase"
                     /protein_id="HEG1169444.1"
                     /translation="MQPEEMPYFVGTVAAIFFQNPSNFYKVLLVHISETTADYREKEI
                     VVTGSFGDIQENETYRFYGELVDHPKYGRQLKLERYEQEKPTTANGIVAYLSSEKFPG
                     IGKKTAEKIVELLGEDAIEKIIADPSVLKQISGLTKPKREMLAETIRLNHGMDQVIMG
                     LNRYGFGSQLAFSIYQAYKNESLDIIEENPYQLVEDIEGIGFKRADNLAEQIGIAADS
                     PRRIRAAILHQIFQHAVQTGDTYVPAETLLQQTIRILETSRPIEIQPDQVATVIIQLV
                     EEGKIQQEETNLYENSLYFSEWGIGNSIQRLLSRKKEIHYPEKEVQKNIRRIEKIFGI
                     QYGPSQEQAIAEAIRSPLFILTGGPGTGKTTVINGIVNLFAELNGLELDPSKYTQEIF
                     PILLAAPTGRAAKRMNETTGLPASTIHRLLGLNGREKTPSVSTKELEGGLLIVDEMSM
                     VDTWLANTLFKAIPTNMQVILVGDKDQLPSVGPGQVLHDLLEIEQIPKMELNQIYRQG
                     DGSSIIPLAHEIKEGRLPKDFTKNQKDRSYFRSDAYQIEPLISKIVEKAKNKGFRAQD
                     IQVLAPMYRGPAGIDALNKMMQEIFNPGKPGKKEVHWNDTVYRIGDKVLQLVNTPELN
                     VFNGDMGEITGIILAKDSEDKVDELVIQFDANEVTYKRNEWNKITLSYCCSIHKSQGS
                     EFKMVILPMVHQYQRMLQRNLLYTAVTRSKELLILLGEEQAYVTCVANESASRMTTLK
                     SRIMDNDKMNITLRTKLAAYEDSLVGNDPFEDGNYTKEPRVVKEKLPVEDDSGTNETK
                     NQVMETVEPDLFSSFVGEEKKEEVEPAVSKDFVLTAEVIDNQLVDPMIGMENSSPYDF
                     IPAKNA"
     gene            complement(6510..7169)
                     /locus_tag="SCJ86_001908"
     CDS             complement(6510..7169)
                     /locus_tag="SCJ86_001908"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine phosphatase family protein"
                     /protein_id="HEG1169445.1"
                     /translation="MELYFTRHGKTQWNQERRFQGSNGDSPLLPQSYEEIKAFGKKVK
                     YVPFKAIYSSTAKRARDTAEGINKELARPVEIFYTDKLRELGLGTLEGQSIDEMYQKY
                     PDNLPNLRNHLDLYDPSPFHGETIESAITRIETVVADAVAQHKDGPLLFVGHGASLTA
                     AIQWMTGKELSELREMGGLFNSSLTILETEEPCRLLPYELKVWNQTDFLGTETAPEPL
                     L"
     gene            complement(7218..7721)
                     /gene="trmL"
                     /locus_tag="SCJ86_001909"
     CDS             complement(7218..7721)
                     /gene="trmL"
                     /locus_tag="SCJ86_001909"
                     /EC_number="2.1.1.207"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (uridine(34)/cytosine(34)/5-
                     carboxymethylaminomethyluridine(34)-2'-O)-
                     methyltransferase TrmL"
                     /protein_id="HEG1169446.1"
                     /translation="MNHIVLFEPLIPANTGNIARTCAATNTPLHLIEPLGFSTDDKHL
                     KRAGLDYWNDVNITYHSNLEAFLTFLGNRKLHLISKFAHKVYSDENFADGEEHFFLFG
                     KETTGLPETFMRENEEKCLRIPMNDEHVRSLNLSNTAAMIVYEALRQQGFPNLELTHH
                     YENDKLD"
     gene            complement(7942..8808)
                     /locus_tag="SCJ86_001910"
     CDS             complement(7942..8808)
                     /locus_tag="SCJ86_001910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HEG1169447.1"
                     /translation="MLKKIDRGRQFLAENHQLFRCPVCQEEVKKSDNGLICKNNHRFD
                     LSKKGTLYFLSHAVKTEYDQGMFVPRRRMIQSGMYQPVIETIAQHLPEEVSLLDVGCG
                     EGSFLNELSKLKKIQNAVGFDISKEGVYAATEQSAEAFWCVADLTNLPFADQTFNAVL
                     NIFSPSHYQEFQRVLSEDGEVLKVVPQENYLKELRQAFYPNQPEKQSYSNERVVAKFA
                     EAMKLTERQRVTYEFEIPEENQKDLLAMSPLEWQVEERIKADLYKNPLKKITIDIELL
                     KGTKRKRFSSSF"
     gene            8917..9558
                     /locus_tag="SCJ86_001911"
     CDS             8917..9558
                     /locus_tag="SCJ86_001911"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375871.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="copper homeostasis protein CutC"
                     /protein_id="HEG1169448.1"
                     /translation="MIKEFCAENYTSIPLAIARGADRIELCDNLAVGGTTPSTGVIEE
                     TLSYAGEKDIPVMTIIRPRGGDFVYNDIELKIMHTDLIEAKKLGTDGVVFGCLTPSGW
                     LDEEALELLIETAEGLQITFHMAFDSIPEDRQFEAIDWLVEHGVDRILTHGGPADKTI
                     VENFPRLKELIAYTDGRLIILPGGGVTAENAAHVAKELNVSEVHGTKIVDLQL"
     gene            complement(9620..10981)
                     /gene="mgtE"
                     /locus_tag="SCJ86_001912"
     CDS             complement(9620..10981)
                     /gene="mgtE"
                     /locus_tag="SCJ86_001912"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294561.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium transporter"
                     /protein_id="HEG1169449.1"
                     /translation="MDENQELEERFAQLTQDLRENNIQAFRNSFLEMHIYEQGQFYQS
                     LTEENRQLIYSYLSPKELADMFDVIEEDNEHMKEYLDEMRPSYAADMLSEMYTDNAVD
                     LLNTLDKKQIAKYLSLMSADDASEIKELLHYEDETAGAIMTTEFVSIVANQTVRSAMY
                     VLKNEADVAETIYYIYVVNQEGQLVGVISLRDLIVNDDDAMISDLMSERVLSVHVGDD
                     QEDVAQTFRDYDFLALPVTDYDDHLLGIVTVDDIIDVIDDEAASDYSGLAGVNVEEIN
                     ENPVKAASRRLPWLITLLFLGMSTASLISHYEDLVSEASILAVFISLITGTAGNAGTQ
                     SLAVAVRRLAISDEKDHGFVRLVLAEVLTGVVTGAVTGITIFLIVGFWKHNFILGFVI
                     GMAMLFAITVANLAGSLIPMLMDRLGFDPAVASGPFITTLSDLTSVLIYFNIASLFMG
                     YFM"
     gene            complement(10999..11898)
                     /locus_tag="SCJ86_001913"
     CDS             complement(10999..11898)
                     /locus_tag="SCJ86_001913"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294562.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RluA family pseudouridine synthase"
                     /protein_id="HEG1169450.1"
                     /translation="MQFTWDYEKDHSQQVKYFLKEKGISKGLLAKIKFQGGQIKVNDQ
                     VENVLFSLAKDDKVTIVIPAEGEHETVLLDETPIDIVYEDEHVLVVNKPAGVSSIPAQ
                     YHPNGTMANRVKAYYKKQGYENQVIHVVTRLDRDTSGLMLFAKHGFAHAKLDMQLREK
                     KFVKKYQALVSGRLSDLSAHGHIDLPIARDYSSLLKRMVSDEGRSAQTEYWIEKRLTE
                     IALVDIQLHTGRTHQIRVHFSAIGCALLGDDMYGGRMDLGIKRQALHCYDLRFYHPFT
                     EELLEFKQPLAADMEAVVRRFEE"
     gene            complement(11900..12697)
                     /locus_tag="SCJ86_001914"
     CDS             complement(11900..12697)
                     /locus_tag="SCJ86_001914"
                     /EC_number="2.7.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323518.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD kinase"
                     /protein_id="HEG1169451.1"
                     /translation="MKVAIVHNKEAKTIEVTERLNALLEQAGIQRDDHEPELVISVGG
                     DGTLLSAFHHYSHCLNDVRFLGVHTGHLGFYTDWRDYELEELVDSLRTNREQSVSYPL
                     LDVRISYLDETPDQHFLALNESTIKRANRTMVADVYIKNELFESFRGDGLTISTPTGS
                     TAYNKSVGGAVIHPSINAFQLAEIASLNNRVFRTLGSPIVIAHDEWLEIKLENTEDYL
                     VTVDQLNVAKANIRSIYYRIAEERIHFASYRHMHFWHRVKDAFISED"
     gene            complement(12675..13379)
                     /locus_tag="SCJ86_001915"
     CDS             complement(12675..13379)
                     /locus_tag="SCJ86_001915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289848.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTP pyrophosphokinase family protein"
                     /protein_id="HEG1169452.1"
                     /translation="MERDWEAFLAPYEQTVSELKVKLRGIRKQFREQNRHVPIEFVTG
                     RVKPVDSIVTKSQLRHIPMGRLEEEMSDIAGLRIMCQFVEDIHQVVEIIRKRKDMKVL
                     QERDYITNKKASGYRSYHLVIEYPVQLISGEKKILAEIQIRTLAMNFWATIEHSLNYK
                     YQGVFPEEMSERLQRAAEAAYQLDEEMSSIREEIQEAQRLFSHGRGKSDDVYYRTLLK
                     KESEREEAGDESSDRS"
     gene            13510..14085
                     /locus_tag="SCJ86_001916"
     CDS             13510..14085
                     /locus_tag="SCJ86_001916"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CYTH domain-containing protein"
                     /protein_id="HEG1169453.1"
                     /translation="MTQMLEIEYKSMLTKQEYEIFIAHYQLTDKDFRVQTNIYFDTVD
                     EQLKKQNCGLRIRFVGHQAEYTLKTPAEKGRLETTDTFITEEAEAFIFEKRLPRSGAV
                     FQKLSDLNIDPASLIQTGKLTTKRAEFPIEEGLLAIDESWGDNLHDFELELEVGDASV
                     GKIAFINFLKRFSLPYRPAKNKIQRMLEAKN"
     gene            14203..14880
                     /locus_tag="SCJ86_001917"
     CDS             14203..14880
                     /locus_tag="SCJ86_001917"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DsbA family protein"
                     /protein_id="HEG1169454.1"
                     /translation="MIEIYLFVNPLGEYCFEMEQQLLQFIEEEYGTPSKEKMQFRFLP
                     LVNLQTIGDVMQQKGISQNNLEERNHLFSMTYSAALDCKAAQFQGKKKGRRFLIKLQE
                     AVGCNDIPYSQELAESIFEEIGGDLNMFKEDRQSDFVKEVFFSDQKIAREMGITKHPS
                     AVVYNYACDRDFGVLVEVKGTSSLWRITELCKTTDNSYQVFNGEHLHTNDSQFHSRTS
                     HLHLLSN"
     gene            complement(14959..16767)
                     /gene="pepF"
                     /locus_tag="SCJ86_001918"
     CDS             complement(14959..16767)
                     /gene="pepF"
                     /locus_tag="SCJ86_001918"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336686.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oligoendopeptidase F"
                     /protein_id="HEG1169455.1"
                     /translation="MEVKQLPKREELPENLTWDLTKIFSSDQEFDEKYLELSEELKQS
                     EKHKGTLDQGASQFLNAIEFVLRVYRQTEVIYVYAHLKNDQDTGNTDYQALYARASSL
                     FSKVSEAVSWFEPEILQLSDDQIWQYFKEEPKLEVYRHYIQQIVDNRAHVLSAEQESL
                     LAGAGEIFDASSDTFAVLNNADLVFPTIEGENGEIVQLSHGVYGQLLESTDRRVREAA
                     FKGLYSVYEQFRNTFASTLGTHIKGHNFKAKVRNYSSAREASLSNNHIPESVYDTLVD
                     VVNKHLPLLHRYMELRKRLLEVEKLHMYDLYTPVLGEAPITFTYEEAKEKALEALKPM
                     GEEYMAIVEKAFSERWIDVVENKGKRSGAYSSGSYDTNPYILLNWHDTLDQLFTLVHE
                     MGHSVHSYFTRSNQPYVYGDYSIFLAEIASTTNENILTEYLLETEKDPRVRAYVLNHY
                     LDGFKGTVFRQTQFAEFEHFMHTEDEKGVPLTSEYLSDSYGKLNAKYYGPAVEEDPEI
                     KFEWSRIPHFYYNYYVFQYSTGFSAASALAKKILNQEPEALENYLAYLKAGNSDYPVE
                     VMKKAGVDMTQAAYIEDAMSMFEQRLNELEELIDRL"
     gene            complement(16943..17941)
                     /locus_tag="SCJ86_001919"
     CDS             complement(16943..17941)
                     /locus_tag="SCJ86_001919"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318540.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="competence protein CoiA"
                     /protein_id="HEG1169456.1"
                     /translation="MLLAMNEYNKCCFAKHAEKGKKYYCRGCRKRVILKKGKKKCAHF
                     AHQKSDNCSVFSERESKEHLQLKECFMDWLGQSVEPVFLEAYLPRLRQRPDILLANLA
                     IEIQCSRLSHQRFIERTQNYLNNSYQVWWILGNSFLGQSQFSLIEKSCCYYNRKRGVH
                     CWKADLKKQKLYLYHHITETVSGHISFCSSCWTFSSRDLKEIFTSNEIKMNQMKKIEK
                     VSEDGKKWLTRQLIHKQKNTVSIQEQCYLRHKHLLHLSSWIYQKSRFFFYLQEQVFLY
                     RMLYEEALENQKVPDFNSWLCQIKEHKREWLFPMIDEEMVYQQFFNECIHLSSLKK"
     gene            complement(18167..18820)
                     /locus_tag="SCJ86_001920"
     CDS             complement(18167..18820)
                     /locus_tag="SCJ86_001920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335220.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adaptor protein MecA"
                     /protein_id="HEG1169457.1"
                     /translation="MEMEHINENTIRVLIGHEDLEERGVSFLDLLGNHKEIENFFYSI
                     LEEVDIEEEFRGSEAVTFQVLPKGDGLELFISKNLPDEEISQSEDSTVDSEDVSDFLR
                     QQIIGSDPEEELSGSALEEQERQATFAFEDFEQVIQLATDVELESAWTDLYLFEERYY
                     LVVHFWMENLNQADVENQIARILEFAEKSDRTPETLNEYGQCLMARNAIERTKFYFV"
BASE COUNT         5525 a         4136 c         3324 g         6141 t
ORIGIN      
        1 acgtaaagct tgctgccaaa ttagcgattg aaactaacga ttgctttcta ggaatcaagg
       61 acaagcgaat accatttgac gattgatttg cttgctgatc ctgtacattg cggacccccc
      121 acatcgtccc gtagagaatc gccattccaa tcagagtgaa gaaataaaaa cttttgaaac
      181 tcatattacg acctgcatga acttcttgaa aagatacttc ttgttcaaaa acagtcatga
      241 tgggcgcgag atcaacgttc tccacttctg ccactttact cgaaattata ccaatacgct
      301 gtaaaaattg atccatgacg tttttcatca acgtctgact cattcctgct tgacccacat
      361 ggagcgaaac agatgaacca tcaatgagat aaaaaccagc caaatcatct gcatttagct
      421 tttcattcgc ttctacttcc gatagttcct gtacttcaaa taatggttta tcattgactt
      481 cgacttgttc taagactgtg gtaaaatttt ccgaaagcgc tgtttgatcc gctttgatta
      541 caattccagt attaatcgtt tcgagattgc caccttgatc aatctcgcca aaggccgcca
      601 taaacatcaa tcctaaaaga attggaaagg ccaatgtcca aaacaacatg gatttatttc
      661 gcagcagtat ttttgtgcga tatttataaa aatgccaaaa cattatttcc cctcctctag
      721 tctctcaaat ccttgccagt aatctctaaa aagacatcat tcaaggacgc ttctttggtt
      781 gctatttttc cgtaactgat cttttcttct tgtaagattg taagaatcgg caacaatact
      841 gtatttagtt cagtagttgt tacttggagc tgaccttttt tataagcgat tccatcagaa
      901 gctattgttc gctgacaaca cgttttctgg gagtaatggg atgtcgatcg tcaaacgatc
      961 cgccgtccgt accatatctt ttagagattc tttggttcct ttagcaatca cctgtccttg
     1021 atccatgatc acgatctggt cgcaaagaat ttctacttct tccatgtaat gtgttgtata
     1081 gacaatggtt gctccttgtc gattcagctc cttgatactt tctaaaattt tattccgact
     1141 ttgggggtct acagctactg tcggctcatc taaaaaaatt aaccgcggct tgtgggcaat
     1201 cccacatgcg atgttcaacc gacgtttcaa gccaccacta agttctcctg gacgaaacga
     1261 aacatatttt tctaatccca ctaattgaat cacctcttca atcaaggctt tccttttctc
     1321 tttttcggaa acatataaac cgcaaaaata atcgatattt tcttgcacac ttagttcgtc
     1381 aaagaccgca acgtcttgag gaacgacccc aatcattgcc ttgacagcat aattttcagg
     1441 agtcattgtt gtcccgaaca atttaatggt tcctttgtca taatttaaca aagaaagcat
     1501 acagttgatg gcagtcgatt tcccactgcc gtttggtcct aacagaccta agacttctcc
     1561 ttcttgtact tgcagatcaa aatgattcaa tgctacaaaa tctccatagc gtttgactaa
     1621 tttatcaatt tcgataatca tcatgaaaaa ctccttccaa atttcttgtt gatctgacta
     1681 taataaagtg caagaaaaga aactagtgac gattggctaa accacacatg acatttgtca
     1741 caatgaagga gtctgctatg cataaatctc ggctactcaa cgaaactagc tttctactgg
     1801 ttatctcatt gcttttgctg gtaagtcaag gattcgttgc tatgcaagtt atctatttat
     1861 tgatcgccgt tttattcact ggtgtcagtt atttactgcc tacaaagcca aattggattt
     1921 tccaagttat tctatttctt ttatgtgcct ttttacctga tcttgtcctg tttctgccag
     1981 cgacgatgcg agtgatcgcc cctgaagaaa gccaccttga ttacttgttt ttgttaggtt
     2041 ttttgttgat ttcgacggtt ggtaatcatc cccccatcaa ggtcgccttt gtcgtaattt
     2101 tgctgttttt ttccttttac ttacgaaaaa aagatcaacg aacgaccgat cttgagaaaa
     2161 agtacattgt ctccaaagat actcattggg aaaaacaaca agagttatcg gaacaaaata
     2221 aagcattggc cctgtcacaa gaaacacaat tagccttgca aattgtggaa gaacggaacc
     2281 gaattgcccg agacatccat gacaatgtcg gtcatctgct gtcaagtgcg attttacaaa
     2341 ttggtgcgct tgaaatgatc aatcaagaac caaaactaaa agaaccatta gaaaatttaa
     2401 gtgccaccgt tcacactggg atggatcgga ttcgtcaaag tgtccatgac ctccatgaga
     2461 catcgatctc ttttcaacac tccttagagt ttctgatcgc tgattttcct tcttctgtta
     2521 cgattgaagg aaacttgttt gaaggattga atgaaacgca gcaaaactgc ttcttaatgg
     2581 tcatcaaaga agccttaacc aacagtatca aacatagtca tgccaaaacc gttgttttac
     2641 attttcgaac gttgccggcc ttttatcgct tgcaaatcac gaatgatggc accagcccac
     2701 aaaaaaattc tgctcccggt attggtttga aaacgatgcg tcaacgtgtt caagaacaaa
     2761 agggacaagt ccatattttc caagaaaacc agcaatttca gttaacgatc attttaccaa
     2821 gggagggaca ccaatgataa cagtagtcat tattgagatg atccatttgt gacgaaatcg
     2881 ttacaaacca ttttagaaag cacgaaagag atccgtgtgt taggcattgg tcattgtgcc
     2941 aaggatgcgc tagtcttata cgaaacccat cgtcccgatg ttttgctgac ggacatccgc
     3001 atgcctaaac aaacgggcat cgatgctgcc aaagaaattt tggctaaatt tcccaaagcc
     3061 atccttttgc ttttgacgac attcaaagat gaagaataca tccgcgaagc attttccatc
     3121 ggtgtcaaag ggtatttaat caaacagaac ttgcaagcga ttattccttc tgtcaaatcc
     3181 gcttataatg gacaggccgt atttggtaat gaaatcatcg agactttcac tcaaatgatg
     3241 aagaataaac cgacgattga tagtcatgct tatgcttctt ttagtgaacg tgagcttgca
     3301 atcatcaaag aagtagctgc cgggaaaaac aataaagaaa ttgcagatgc cttgtacttg
     3361 agtgacggca ctgttcgcaa ttacatcagc caattactag aaaagctaga tttacgggat
     3421 cgcacgcaat tggctatcta tttttatcag catttgcaat aagtcttatc taacacaaaa
     3481 aaacctgaat gatgctcctt cgcatcattc aggttttttt cacatcttct attcaattgt
     3541 cccgcaaacc atttcctcac gattgaaaga atcaaggatt cgcagggctg ccaggattcg
     3601 ttggattgtt cggcgtgtta ggcggaaaaa taggcgcctt gtctaacgtt ttttctagtt
     3661 ttgattcttc acgatcgtct cgttctacat caggactttc attcaatttg aattcttttt
     3721 ccatctcatc taacttttta aagtcattgc cagattcttt tgctaatttc gctaatttat
     3781 cttcggtttc tttagccata aagtaacgcc ccccttttga tttcagctta gcatatcaca
     3841 ctgaaaaaac aaaagaaaac gctaaccaac gatcattttt agctatttct catctttaaa
     3901 atcaagcatt ttttgccggt ataaaatcgt agggagaact gttttccata ccaatcatcg
     3961 gatctactaa ctggttatcg atcacttctg ctgtaagaac gaaatcttta gaaacagcag
     4021 gttctacctc ttctttcttt tcttctccaa caaatgaaga aaacagatct ggttccactg
     4081 tttccattac ttggtttttc gtttcattcg tcccactgtc atcttctaca ggtagctttt
     4141 cttttacaac ccgtggctct tttgtatagt tcccatcttc aaatggatca tttccgacca
     4201 aagaatcttc ataagctgct agtttggtcc ggagagtgat attcatttta tcattatcca
     4261 tgatccttga tttcaacgta gtcatccggc ttgctgattc attcgctaca caagtgacat
     4321 aggcttgctc ttcccctaac aaaatcaaca gttccttact gcgagtcact gccgtgtaga
     4381 gcaaattacg ctgaagcatc cgctgatatt gatggaccat gggcaaaata accatcttga
     4441 attcactgcc ttgtgattta tggattgagc aacaataaga aagagtgatt ttattccatt
     4501 cgtttctttt ataagtgacc tcatttgcat caaactggat aactaattca tccactttat
     4561 cttcagaatc ttttgccaag atgatacccg tgatttctcc catatctcca ttgaatacat
     4621 tcaattcggg agtattgact aattggagga ctttgtctcc tatccgataa acggtgtcgt
     4681 tccaatgtac ttcctttttt ccaggtttcc ctggattgaa tatctcctgc atcattttat
     4741 ttaacgcatc gatccctgca ggtccccgat acattggtgc taatacttgg atatcttgtg
     4801 cacggaatcc cttatttttc gctttttcca caattttaga gatcaagggt tcaatttgat
     4861 aagcatcact tcggaaatag gaacgatctt tttgattctt tgtgaaatct tttggcagac
     4921 gtccttcttt gatctcatgg gcaagaggaa taatactaga accatcacct tgccgataaa
     4981 tctgattgag ttccattttg ggaatttgtt cgatttccaa aagatcatgg agtacttgac
     5041 caggaccgac agagggaagc tgatctttgt ctcctactaa gataacttgc atattcgtcg
     5101 gaatcgcttt gaataatgtg ttagccagcc atgtatcgac catagacatc tcatcaacaa
     5161 tcaaaagtcc gccttctaat tctttcgtac tgactgaagg tgttttttcc cgtccattca
     5221 gccccagaag acgatggatc gtactggcag gcagacctgt cgtttcgttc attcgtttag
     5281 ctgctcggcc agttggtgct gcaagtagaa taggaaaaat ttcttgggta tattttgaag
     5341 gatccaattc cagtccgttc aactctgcaa aaagattgac gattccgttg atcacagtag
     5401 ttttccccgt acctggtcct cctgttaaaa taaatagagg ggaacggatt gcttcagcga
     5461 tagcttgttc ttgcgaaggt ccatactgta tgccgaatat tttttcgata cgccgaatat
     5521 tcttctgcac ttctttttca ggataatgga tttctttttt acgactgaga aggcgctgga
     5581 tcgaattacc gatcccccat tcagaaaagt acaaactgtt ttcgtaaaga ttagtctctt
     5641 cttgttgtat ctttccttct tcaacaagtt ggataattac agtagcaacc tgatcaggct
     5701 ggatttcaat tgggcgactt gtttctaaaa tacggatcgt ttgctgaagc aatgtctccg
     5761 ccggtacgta tgtatctcct gtttgaactg catgctgaaa aatctgatgc aagatagctg
     5821 ctcgaattct tctcggtgaa tctgctgcaa taccgatttg ttcggctaga ttatctgcac
     5881 gtttgaaccc gattccttct atgtcttcga ctagctggta cggattttct tcgatgatat
     5941 ccaaagactc gttcttatag gcctggtaga tactgaaagc taactgacta ccgaacccgt
     6001 aacgattcag tcccatgatc acttgatcca tcccgtggtt tagccgaatc gtctcagcaa
     6061 gcatttctct ttttggcttc gtcaaaccgg agatttgttt taataccgaa ggatcagcaa
     6121 tgattttttc gatagcatct tctcccagca attctacaat tttttctgct gttttcttcc
     6181 cgattcctgg gaatttttcg ctggaaagat acgctacgat cccatttgca gttgttggct
     6241 tttcttgctc atatctttcg agtttaagct gccttccgta tttaggatga tccactaatt
     6301 ctccataaaa acgataagtc tcattttctt gaatatcgcc gaaacttccg gtgactacaa
     6361 tctctttttc acggtaatca gctgtggttt cactgatgtg taccaaaagc actttgtaaa
     6421 aattactagg attttgaaaa aaaatcgcgg caactgtacc aacgaaataa ggcatttctt
     6481 ctggctgcat cggaagccgc ctccttcttc tatagtaaag gttctggtgc tgtttcagtt
     6541 cctaaaaaat ctgtttggtt ccatactttc agttcatatg gcaataatct gcaaggctct
     6601 tcagtctcta aaatcgtcaa actactattg aacaatccac ccatttcacg caactcactt
     6661 agctcttttc ctgtcatcca ttgaatggct gcagttaaag aagctccatg tcctacgaat
     6721 aaaagtgggc catctttatg ttgggcaacc gcatctgcta caacagtttc gatacgtgtg
     6781 attgccgatt caatcgtttc tccgtgaaaa ggagaaggat catacagatc taaatgatta
     6841 cgcaaattcg gcagattatc cggatatttc tggtacattt catcgatcga ctgaccttct
     6901 aaagtaccca gccctagttc tcttagctta tctgtataaa aaatctctac cggacgagca
     6961 agctctttgt tgataccttc tgctgtatca cgtgctcgct ttgctgtact ggaataaatt
     7021 gctttaaaag gaacatattt tacctttttc ccaaatgctt tgatttcttc gtaactttgt
     7081 ggcaaaagcg gagaatcgcc attgctccct tgaaaacgtc tttcctgatt ccattgtgtc
     7141 ttcccatgtc gggtaaaata tagttccata cgcagcaaac tcctgtcttt cttttatttt
     7201 actctataat aaactgtcta atccagcttg tcattttcat agtgatgcgt caattctaaa
     7261 ttagggaatc cttgctggcg aagtgcttca tacactatca ttgcagctgt attcgataaa
     7321 ttcaatgagc ggacatgctc atcattcata gggatgcgta aacacttttc ttcgttttca
     7381 cgcataaatg tttctggcaa accggttgtt tcttttccaa ataaaaagaa gtgctcttct
     7441 ccatcagcaa aattttcgtc actatatact ttatgggcaa attttgagat caaatgtagt
     7501 ttacgattac ctaaaaatgt taaaaaagct tctaaattac tgtggtaagt aatatttaca
     7561 tcattccaat aatccaaccc tgcccgtttt agatgtttgt catcagttga aaaacctagt
     7621 ggctcgatta aatgaagcgg agtattcgtg gctgcacacg tacgggcaat atttccagtg
     7681 tttgcaggga ttaatggttc aaataaaacg atatgattca tcaggatcat tcctctcata
     7741 tttatgttcg tttctatttt ctgacaattt gatttttgca taaaaaaaaa cgtatcaact
     7801 cggtgtcagt cactgcgagt cgttacgtta gtgaagctct tctcactaac ttttatcaga
     7861 acaggaacct gataaaaacc aatgaaaaaa caacttgttg ctaatataac accctcaact
     7921 tttttttgca acctctactc attaaaaact gctagaaaaa cgttttctct tagttccttt
     7981 taataattct atgtcaattg taatcttctt caaaggattc ttatataagt ctgctttgat
     8041 tcgctcttct acctgccatt ctaaaggtga catggccaaa agatcttttt gattttcctc
     8101 tggtatctca aattcatagg ttacacgctg acgttcagtt aatttcattg cttcagcaaa
     8161 cttggctact acacgttcat tggaataact ctgtttttca ggttgattcg gataaaaagc
     8221 ctgacgtagt tcctttaaat aattttcttg tggcaccacc ttcaacactt ctccatcttc
     8281 tgagagtaca cgttggaatt cttggtaatg agaaggcgaa aagatattta aaaccgcgtt
     8341 gaatgtttga tcagcaaacg gcaaattggt caaatcagct acacaccaaa atgcttcggc
     8401 agattgttct gtagctgcgt agactccttc ttttgaaata tcaaatccaa cagcattttg
     8461 aatctttttc aattttgaaa gttcattcaa gaagctacct tctccgcagc caacatctaa
     8521 tagcgagact tcttcaggta aatgctgcgc aatagtctca ataacgggct gatacatgcc
     8581 agattggatc attctacgcc ttggaacgaa cattccttga tcgtattctg ttttaaccgc
     8641 atgggataaa aaataaagtg ttcctttttt ggaaaggtca aaacggtgat tatttttgca
     8701 tatcaagcca ttatcagact tttttacttc ttcttggcaa accggacagc ggaacagctg
     8761 atgattttct gccaaaaact ggcgtcctcg gtcaattttt tttaacatag ttccccctca
     8821 tttctgtttt atcttaccgt tagatgctca ctttggcaac tgctaaaaat atgatacaat
     8881 gcctagaaga aagtgaaaga taaaggagaa gattagatga tcaaagaatt ttgcgctgaa
     8941 aactacacat ctattcctct agcaattgct agaggagccg atcggatcga attgtgtgac
     9001 aacttagcgg tcggtggcac aaccccgagt actggtgtga tcgaagaaac gttaagttat
     9061 gctggggaaa aagatatccc cgtaatgacg attattcgtc ctagaggggg cgacttcgtc
     9121 tataacgata tcgaattgaa aatcatgcat actgatttga ttgaagcaaa gaaattaggg
     9181 acggatggcg tagtatttgg ttgtcttact ccttcaggct ggctagacga agaggcctta
     9241 gaactattga tcgaaacagc agaagggttg cagatcacct tccatatggc ttttgacagt
     9301 attcctgaag atcggcaatt cgaagcaatc gattggctgg tagaacatgg tgttgaccgt
     9361 atcttgactc atggcggtcc tgctgacaaa actattgtag aaaatttccc acggcttaaa
     9421 gaattgattg cctatacaga tggccgctta atcattttgc ctggtggtgg tgtcactgca
     9481 gagaatgcag cacatgtagc taaagaattg aacgtaagcg aagtacacgg aaccaaaatc
     9541 gtggatcttc agttataatc ttataaacaa aaaaacattt gcaacaggca taatgcttat
     9601 tgcaaatgtt tttgtttttt tacataaaat agcccataaa taaagaagca atattgaaat
     9661 aaatcagtac actcgtcaaa tcgctcaaag tcgtgataaa gggaccgctt gctactgctg
     9721 gatcaaatcc tagacgatcc attagcatcg ggattaaact acctgcaaga ttcgccactg
     9781 tgattgcaaa tagcatagcc atccctatga caaaccccaa gataaaatta tgtttccaaa
     9841 aacctacgat tagaaaaatc gtgatccccg taacagctcc tgtgacaacg ccggtcaaga
     9901 cttcagccaa aacaagtcga acaaatccat gatctttctc atcagaaatc gcaagtcgac
     9961 gaacagccac agctagcgac tgggttccag cattaccggc agttcctgta atcagcgaaa
    10021 tgaagactgc taaaatactc gcttcactta ccaagtcttc atagtgactg attaaagaag
    10081 cggtcgacat acccaaaaac aacagagtga tcagccaagg caaacgtcga gacgctgcct
    10141 ttactggatt ttcattaatt tcttccacat tgaccccggc aagtcctgaa taatcgctag
    10201 cagcttcatc atcaataacg tcgatgatat catcgactgt gacgattcct aacaaatgat
    10261 catcataatc ggtaacagga agagctaaga agtcataatc tctaaatgtt tgtgccacat
    10321 cttcttggtc gtcgcctaca tgaactgaaa gcactcgttc gctcatcaga tcagaaatca
    10381 ttgcgtcgtc atcattcacg attaggtcgc gcagagaaat aacccctact agctgacctt
    10441 cttgattaac gacatagatg taatagattg tttcagctac atctgcttcg ttcttcagca
    10501 cgtacatcgc agagcgaaca gtttgattgg ctacgatcga aacaaattca gttgtcatga
    10561 tcgcgccagc cgtttcatcc tcgtaatgga gaagttcctt gatttcactg gcatcgtctg
    10621 ccgacatcaa acttaaatat ttagcaattt gctttttatc cagcgtatta agcaagtcaa
    10681 cagcattatc tgtatacatc tcagaaagca tatcagctgc atagctagga cgcatttcat
    10741 ctaggtattc tttcatatgt tcgttgtctt cttcaatcac atcgaacata tccgctaatt
    10801 ctttaggcga cagataggaa tagatcagct gcctgttctc ttctgtaaga gattggtaga
    10861 attgcccctg ctcatagata tgcatctcta aaaaactgtt tctgaatgcc tgaatattat
    10921 tttcccttaa atcttgagtc aactgggcaa atctttcctc taattcttga ttttcatcca
    10981 aaccaatctc acctcgcctt attcttcaaa ccgtctcaca actgcctcca tgtctgcggc
    11041 taaaggctgc ttgaactcca gcaactcttc tgtaaatgga tggtaaaaac gcagatcata
    11101 acaatgaagg gcttgtcttt ttattcccag atccattcgc ccgccataca tatcatcacc
    11161 gagcagggca cagccaattg cggaaaaatg aactcttatt tgatgtgtcc ttcctgtatg
    11221 cagctggata tcaactaacg cgatttctgt tagtcgcttc tctatccagt actcagtctg
    11281 tgccgaacgt ccttcatcag agaccattcg cttcaatagc gaagaataat ctcgggcgat
    11341 cggcaaatcg atatgtccat gtgccgataa atctgaaagc cttccactga ctaaggcctg
    11401 atattttttc acaaacttct tttcacgaag ttgcatatct aattttgcgt gcgcaaatcc
    11461 atgctttgca aaaagcatca aaccagatgt atcccgatct agtcgcgtca ccacatggat
    11521 tacttggttt tcgtaacctt gttttttata ataagccttt acacgattag ccattgtccc
    11581 atttggatgg tattgggcag gaatagacga aacgcctgct ggtttattta ctaccagaac
    11641 gtgttcatct tcatacacga tatcgatcgg tgtttcatcc aaaagtacgg tttcatgttc
    11701 tccttcagca ggaatcacaa tggttacctt atcatcctta gctagagaaa acaagacatt
    11761 ttctacttga tcattaactt tgatttgacc gccttgaaat tttattttcg ctaacagacc
    11821 ctttgagatc cctttttctt ttaaaaaata ttttacttgc tgagaatgat ctttctcata
    11881 atcccatgta aactgcatat caatcctcac taataaaggc atctttcaca cgatgccaaa
    11941 aatgcatatg tcgataagaa gcaaaatgga tccgttcttc tgctattcga taataaattg
    12001 agcggatatt tgcttttgcg acattcaatt gatcaaccgt tacaagataa tcttctgtat
    12061 tttccaattt tatttctagc cattcatcat gggcaatcac gatcggagaa cccaaagtac
    12121 gaaacactcg attattcagt gaagcaatct ctgccaactg aaaggcattg atactaggat
    12181 ggatcacagc accgccgaca cttttattat atgctgtcga accagtaggc gtagaaatcg
    12241 tcaatccatc tcctctaaaa ctttcaaaca gctcgttttt gatgtataca tcggctacca
    12301 tcgtacgatt ggcccgttta atcgttgatt cattcagtgc taaaaaatgc tggtccggtg
    12361 tttcatccaa ataactgatc cgaacatcca gcaagggata actaacactt tgttcccggt
    12421 tcgttcgtaa gctgtctact agttcttcca gctcgtaatc ccgccaatcc gtgtaaaacc
    12481 ctaaatgacc tgtatgcacg cccaagaaac gcacatcatt cagacaatga ctgtagtgat
    12541 ggaacgccga caataatgtg ccgtctccgc cgactgagat gaccaattct ggctcatgat
    12601 catctcgttg aatccctgct tgttctaaaa gtgcgttcaa acgttctgtc acttctatag
    12661 tctttgcttc tttattatga acgatcgcta ctttcatccc ctgcctcctc tctttcgctt
    12721 tctttcttca aaagcgttcg atagtagaca tcatctgatt ttcctcttcc atgggagaat
    12781 aaccgctgtg cttcttggat ttcttcacgg atactggaca tctcttcatc caactgatag
    12841 gcagcttctg ccgcacgctg aagtcgttca ctcatttctt ctggaaaaac gccttgatat
    12901 ttataattca gcgaatgctc aatcgttgcc cagaaattca tcgcaagagt ccgaatctgg
    12961 atttccgcca aaattttctt ctcaccgctg atcagctgta caggatactc gatcaccaga
    13021 tgataagaac ggtagccact tgctttttta tttgtgatgt aatctcgttc ttggagaact
    13081 ttcatgtctt ttcttttacg gatgatctca acaacttgat ggatatcttc tacaaactga
    13141 cacatgatcc ttaacccggc aatatcagac atttcttctt ctaaacgtcc cattggaata
    13201 tgccgtaatt gtgattttgt tacaatacta tcgactggct tgactctccc agtgacaaat
    13261 tcaatcggga catgtcgatt ttgttcacgg aactgtttgc ggatcccccg cagtttcact
    13321 ttcaattctg atacagtttg ttcatatggt gctaaaaatg cttcccaatc tcgttccata
    13381 gctgatacct ctacttcatt aacattcatt gtttattcta ccatatatgc aaagtttttt
    13441 cgtactcttg atttatgcat tgaccaaaaa aagaaaatta ggcaaaatag acaaaaagga
    13501 gtcgatcaaa tgacacaaat gcttgaaatc gaatataaat ctatgctgac aaagcaagag
    13561 tacgagatat tcatcgctca ctatcaatta acagataaag atttccgtgt acagactaat
    13621 atttatttcg ataccgttga tgaacagctg aagaaacaaa attgtggcct acggatccgt
    13681 ttcgttggtc atcaagctga atatacatta aaaacacctg ctgaaaaagg gcgtctcgaa
    13741 acaacggata cctttataac agaagaagca gaagcattta tcttcgaaaa acgtctgcct
    13801 cgttcaggcg cagtttttca aaaactttct gatttgaata tcgatcctgc ttcattaatc
    13861 caaaccggaa agcttacaac aaaacgggct gaattcccta ttgaagaagg acttttagct
    13921 attgatgaaa gctggggaga caatctacac gattttgaac tggaactaga ggtaggggat
    13981 gcatctgtgg gaaaaattgc ttttataaat tttttaaaac gtttttcttt accttatcgt
    14041 cctgctaaga acaagatcca acgtatgctt gaagctaaaa attagaaacc tttcattttc
    14101 acaaggcact tgagttcatt tttttacttt tttctgttaa gttataggat gtgaaagaat
    14161 ctagtcacta gttgtataga atctattttg ggggagattc atatgatcga aatctattta
    14221 tttgttaatc ctttagggga atactgtttc gaaatggaac aacagttatt acaatttatc
    14281 gaagaagagt acgggacacc atcaaaagaa aagatgcaat ttcgctttct tcctttggtc
    14341 aatttgcaga caattgggga tgtcatgcaa caaaaaggaa tttctcagaa caatcttgag
    14401 gaacgtaatc acttattctc aatgacttat tctgcagcat tggactgcaa agcggcgcag
    14461 tttcaaggaa agaaaaaagg ccgccgtttt ttgatcaaat tgcaagaagc agtcggctgt
    14521 aatgacatac catattctca agaacttgct gaatccatat ttgaagaaat cggcggcgat
    14581 ttgaatatgt ttaaagaaga tcgtcagtct gacttcgtta aagaagtatt cttttctgat
    14641 caaaaaattg ctcgggaaat gggcatcaca aaacaccctt ctgcagtcgt ttacaattat
    14701 gcctgcgacc gtgattttgg tgttttagta gaagtaaaag gtacaagtag cctttggaga
    14761 atcacagagc tgtgtaaaac aacagataac tcatatcaag tttttaatgg tgaacattta
    14821 catacaaatg attctcagtt ccattcaaga actagccatt tacacttgct ttctaattaa
    14881 aaaaatacgt cgactccctt acaagagagc cgacgtattt ttttcgtttc tattcgagaa
    14941 aatcaaaaat agaaagcctt ataaacgatc gatcaattct tctaattcat ttagacgttg
    15001 ttcaaacatc gacattgcat cttcaatata tgcagcctgt gtcatatcaa ctcctgcttt
    15061 tttcattact tccacaggat aatcgctgtt acctgctttt aggtaagcca agtagttctc
    15121 tagtgcctct ggttcctggt ttagtatttt cttcgccagt gctgaagcag cagaaaagcc
    15181 agtcgagtat tggaaaacat agtaattata ataaaaatgc ggtatccgtg accattcgaa
    15241 tttaatttct gggtcttctt cgactgctgg accataatat tttgcattca atttaccata
    15301 actatcactt aggtattcac tagtcaatgg cacacctttt tcatcttccg tatgcatgaa
    15361 atgctcgaat tcagcgaatt gtgtctggcg gaaaactgtt cctttaaacc catccaagta
    15421 atggttgagt acataagccc gtacacgagg gtctttttct gtttccaata aatactccgt
    15481 taggatattt tcattcgttg tcgaagcaat ttctgctaaa aagatggagt agtcgccgta
    15541 cacataaggc tggttcgaac gagtgaaata actatgaaca ctatgtccca tttcgtggac
    15601 aagcgtaaat agctgatcca gcgtatcatg ccaattcaat aaaatatatg gatttgtgtc
    15661 atagcttccc gaagaataag caccgcttcg tttcccttta ttttcgacaa catcgatcca
    15721 acgttcagag aatgcttttt ctacgatggc catgtattct tcacccattg gtttcagtgc
    15781 ttctaaagct ttttcttttg cttcttcgta cgtaaaggta attggagctt cccctaagac
    15841 cggtgtataa agatcataca tgtgcagttt ttccacttct aataaacgtt tccgtaattc
    15901 catgtatcga tgtaacaaag gcaaatgctt gtttaccacg tctaccaaag tatcgtatac
    15961 actttcagga atatgattat tgctcaaaga cgcttctctg gcagagctgt aattacggac
    16021 tttcgcttta aaattatgtc cttttatatg tgtgcctaaa gtagaagcaa atgtatttct
    16081 aaattgttcg taaacactgt acaatccctt aaatgctgct tcgcgcaccc ttcgatccgt
    16141 gctttctagc aactgaccat acacgccatg agataattgg actatttcac cattttctcc
    16201 ttcaatcgtt ggaaaaacta gatctgcatt attcaaaaca gcaaatgtat cacttgaagc
    16261 atcaaagatt tcacctgctc cagcaagaag agattcctgc tcagcagata agacatgggc
    16321 tcgattatct actatttgct ggatataatg gcgatagact tccaattttg gttcttcttt
    16381 gaaatattgc caaatctggt catctgacaa ttgcaatatt tctggttcaa accaggaaac
    16441 ggcttcacta actttcgaaa acagactgct tgctcttgca taaagcgctt ggtaatctgt
    16501 atttccagtg tcttgatcgt ttttaaggtg cgcatataca taaatgactt cagtttggcg
    16561 ataaaccctc aatacgaatt caatcgcatt taaaaattga gaagcgcctt gatcaagtgt
    16621 tcctttgtgt ttttcagatt gttttaactc ttctgataat tccaaatatt tctcatcaaa
    16681 ctcttggtcg cttgaaaaga tcttggtcaa gtcccaagtt aaattttcag gcaattcttc
    16741 tcgttttggc aactgcttta cttccatgaa atccactcct tttcttccct tagctctatc
    16801 tatcaaaata gatcatagtc taacatccac atgtcacagc atctaacttt tctactcatt
    16861 catgtgtctg cttccatcaa ttttagcata actaaatctt gttttacata gaaaaccgat
    16921 tcctagccta tttttatact tactattttt tcaaagaaga caagtgtatg cattcgttaa
    16981 aaaactgctg atagaccatc tcttcatcaa tcataggaaa aagccattcc cttttatgtt
    17041 cttttatttg gcaaagccat gagttgaaat caggaacttt ctggttttcc aaggcttctt
    17101 cgtacaacat tcgataaaga aaaacctgtt cttgtaaata aaagaaaaac cgactttttt
    17161 gatagatcca cgatgataga tgcaaaagat gtttatgacg caagtagcat tgttcttgta
    17221 tggatactgt attcttttgc ttgtgaataa gttggcgtgt cagccatttc ttcccatctt
    17281 cactcacttt ttctattttt ttcatttggt tcattttaat ttcgttacta gtgaatattt
    17341 ctttaaggtc cctactagaa aaagtccagc agctactgca aaaggaaata tgccctgata
    17401 cagtctctgt aatatgatga tacaaataaa gcttttgttt cttgagatca gctttccagc
    17461 aatgcactcc tctttttcta ttatagtagc agcaactttt ttctatcaat gagaattgcg
    17521 attgtccaag aaaagaatta ccaagtatcc accatacttg gtaactgtta ttcaaataat
    17581 tttgggttct ttcaataaat ctctggtgag acagtctaga acattggatt tctattgcta
    17641 gattcgccaa caaaatatcc ggcctttgcc gcaacctcgg caaataggct tccaaaaata
    17701 ctggttctac tgactgtccc agccaatcca tgaagcattc ctttaactgt aagtgctctt
    17761 ttgattctct ttcactaaag accgaacagt tatctgattt ttgatgggca aaatgagcac
    17821 attttttctt tccctttttc aaaatcactc tttttcgaca gccacgacaa taatatttct
    17881 ttcctttttc tgcatgttta gcaaaacagc acttattata ttcattcatt gcaagcagca
    17941 tttttctccc tctgaactta tttacgtcta agaatttatt tacacgaaaa aagagggcgt
    18001 gaaaaagcta ttctgcatca agtaataaga acagctaaac aaaaatagtt cccaatattt
    18061 ttcgtttggt tgggcttgtt accgcagatg caagcttttg aacacattta tacgaaaaaa
    18121 agactgcgac acaatttttg tcacagtctc ttactataat gctgatttat acaaaataaa
    18181 atttcgttcg ctcgatagca ttacgcgcca tcagacattg tccatattca ttcaatgttt
    18241 ctggtgtacg atcgctcttt tcagcaaact ccaaaattcg agcaatctga ttctctacat
    18301 ccgcttggtt gagattttcc atccagaagt gaacgactag gtaatagcgc tcttcgaata
    18361 aatacaaatc agtccaagca gactctagtt cgacatctgt tgcaagttgg atcacttgtt
    18421 caaaatcttc aaaagcaaaa gttgcttgtc tttcctgttc ttctagggcg cttccagata
    18481 attcttcctc cggatcgctg ccaataatct gctgacgaag aaagtcactc acatcttctg
    18541 aatcaacagt agaatcttct gattgagaaa tttcttcatc cggaagattt ttactgataa
    18601 acagttctaa tccgtctcct tttggcaaaa cttggaaagt gacagcttcg cttcctcgga
    18661 actcctcttc gatatcaact tcttccaaaa tactataaaa gaaattttcg atttctttgt
    18721 gattgccaag taaatccaaa aatgaaacgc cccgttcctc taaatcttca tgaccaatta
    18781 gtacacggat cgtattttca ttaatatgtt ccatttccat tttgctacac ctcactttgc
    18841 cacaatgttt aatatagatt cattgtagcg aaatcagctt aaatgtaaag aaaaaaaatc
    18901 tcgctgttaa gcgaatttaa taaaaccagc ggatttttca acctttcaag ctatcttatt
    18961 taactataac atttttggca gacgaatact atatagccga cttttcagct aattttccct
    19021 ctctattttt cgtgcaatca tcatcttcta ctaccttttt atacatctaa tcaagaatac
    19081 tttttgtaaa aacaaaaaaa cttggaaaaa ctatctttgt aatagt
//
LOCUS       DARBET010000051        18740 bp    DNA     linear   BCT 07-NOV-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4027
            SAMEA114569841-rid20112183.denovo.052, whole genome shotgun
            sequence.
ACCESSION   DARBET010000051 DARBET010000000
VERSION     DARBET010000051.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA114569841
            Sequence Read Archive: ERR12203666
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 18740)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 18740)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001972618.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001972618.1
            Genome Coverage       :: 132x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/07/2023 13:18:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,041
            CDSs (total)                      :: 2,975
            Genes (coding)                    :: 2,908
            CDSs (with protein)               :: 2,908
            Genes (RNA)                       :: 66
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 67
            CDSs (without protein)            :: 67
            Pseudo Genes (ambiguous residues) :: 0 of 67
            Pseudo Genes (frameshifted)       :: 26 of 67
            Pseudo Genes (incomplete)         :: 28 of 67
            Pseudo Genes (internal stop)      :: 20 of 67
            Pseudo Genes (multiple problems)  :: 6 of 67
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18740
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA114569841-rid20112183.denovo.052"
                     /isolate="VRE4027"
                     /db_xref="taxon:1352"
                     /country="Denmark"
     gene            256..531
                     /locus_tag="SCJ86_001921"
     CDS             256..531
                     /locus_tag="SCJ86_001921"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337751.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEG1169458.1"
                     /translation="MRESFEQQKKLLHDRYGALSMDDRRQILCKLRKRNILMYRQLER
                     LKHDLLRLESKRVQCELEGNQTQVEVVETKILKKKEQFLKMLTQNKK"
     gene            544..2616
                     /locus_tag="SCJ86_001922"
     CDS             544..2616
                     /locus_tag="SCJ86_001922"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324658.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sodium:proton antiporter"
                     /protein_id="HEG1169459.1"
                     /translation="MEFAELVIVFAVTITLSNIASRILPMIPAPLIQIFLGVVLGLTE
                     WGQSIDFEPELFLVMIIAPLLFREGEKADISSILKNFGTILFLAFGGVILTLVGVGAT
                     LSFLLPSVPLAACFAFGAALGPTDAVAVSSLSGRVNIPKKAMHILEGEGLLNDASGVT
                     AFQFALGALITGSFSAVNAGMSLVVSSIGGALIGFLLVWFKQKIIHLIEKASAQDVTG
                     YLLIELLLPFLAYVLAEAFDVSGIIAAVAAGILQASGFRKISVFDAELSSLSHSTWTT
                     IAFTLNALVFIFLGIELTQVFSPVWGDGLYPNGLLLAIIVLISVMLFVIRFISISLFY
                     VFKDGSKKFKKQLNEILILTFGGVKGTVSLATIFILPFSINNMMFYQRSLLLFLTAGV
                     ILVTLVIGIIVLPMLTETEEAKSTDLNALMILEEVVEVLRKEIKEIDRNTKEFLATEA
                     VIENYQERIRDLYLEDLTDDEKQEVQEIQALILSIERDGLDESYRSGKLSSNGYRFYS
                     RFLSRFEHSITSQILSFIGFWFIVVRRLMRIVLHPKMFWQRRYADRQTFISDEDIQEI
                     RETYQKNTQLIIESLDNLTDIYDETMVQFFIQQRKLEGIKMNSGNLISSWMIQQDSLF
                     TKKMLRGYYLERKIIDEYEVAEKITTFSANSYRRNINLLESYTMNKPSDNFSFRFAFR
                     AKSKKRVQ"
     gene            2781..4667
                     /locus_tag="SCJ86_001923"
     CDS             2781..4667
                     /locus_tag="SCJ86_001923"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324144.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F family ATP-binding cassette
                     domain-containing protein"
                     /protein_id="HEG1169460.1"
                     /translation="MLKELKVTDLKKTYGEKDLFDQISFLVHEKDRIGLIGTNGTGKT
                     SLLNIIAGIDSSDGDRQTVFYPTDYRIGYLSQAAEFSDELTVLQAVFQGNSPLIQTVR
                     AYEEALIELGENGEDPDVQKRYAKAEEQMNKEDAWTTDTNAKIILQKLGISELDKKIS
                     TLSGGQKKRVSLAQVLIDEPDLLLLDEPTNHLDYEAIEWLENYLKQYRGALLMVTHDR
                     YFLDRVANRIFELSFGKLYEYKGNYEAYVLEKAERDRVEVEQEEKRKRLYKQELAWMR
                     AGVQARGTKQQARINRFEDLKENLYQVNQEDDLELNLATQRLGKKVLEIKDGSYRIND
                     QTLLEHLDLLIQSRERLGITGKNGAGKSTLLNILAGRIPLDSGTMSIGETVHLAYYTQ
                     ENEEMAPDKRMIAYLQEAAEEAKTADGSQIGVAELLERFLFPRFMHGTLIGKLSGGEK
                     RRLFLLKLLIQQPNVLLLDEPTNDLDIATLTILEDYFRSFPGAVITVSHDRYFLDKVA
                     DKLLVFQGNGKQELYYGNMSSYLLKQKETQQPAEKAKPKIQSKEPAEKKKLSYMEQKE
                     WETIEDEIAELEEKISLLQEEMNHQGDNFTRLQELQNDVSETEAQLEEKMARWEYLSE
                     WVED"
     gene            4679..5626
                     /locus_tag="SCJ86_001924"
     CDS             4679..5626
                     /locus_tag="SCJ86_001924"
                     /EC_number="2.1.1.45"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294416.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thymidylate synthase"
                     /protein_id="HEG1169461.1"
                     /translation="MEQAYLDLGRKLLEEGHRKEDRTGTGTLSLFGYQMRFDLQKGFP
                     LLTTKRVPFGLIKSELLWFLKGDTNIRYLLQNKNHIWDEWAFERYIKSPDYNGPDMTD
                     FGHRCLTDPEFNEEYKKQSKLFCEKILTDDSFAETYGDLGHIYGYQWRHWETKDGGFI
                     DQIKQVIEAIKKTPDSRRLIVSAWNPEDVPSMALPPCHTMFQFYVQEGRLSCQLYQRS
                     GDVFLGVPFNIASYALLTHLIAHETGLAVGEFVHTLGDAHLYINHIEQMKEQLSREIR
                     AFPTLVLNQEKQSVFDFEMEDIQLEGYDPHPAIKAPIAV"
     gene            5646..6173
                     /locus_tag="SCJ86_001925"
     CDS             5646..6173
                     /locus_tag="SCJ86_001925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306927.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrofolate reductase"
                     /protein_id="HEG1169462.1"
                     /translation="MLAAIWAQDENGLIGKEDRLPWRLPNDLKFFKQMTEANTLVMGR
                     KTFEGMGSRPLPNRQTIVLTRDSSYKAEGVHVMHSVEDVLALEKETDGIFFIAGGSAV
                     YQEFLPFCTILYRTVIHHVFDGDAYFPPVDWSDWSLINTSQGEMDEKNRYPHQFETYQ
                     RNENPVKSGTGELTI"
     gene            complement(6236..6889)
                     /locus_tag="SCJ86_001926"
     CDS             complement(6236..6889)
                     /locus_tag="SCJ86_001926"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347222.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hemolysin III family protein"
                     /protein_id="HEG1169463.1"
                     /translation="MEKTKFNRTYEILNEVFNAVTHGIGTGLSIAGLVVLLVKGAHLH
                     SSLHIVSYAIYGSMMILLFLTSTLFHSLIFTKAKKVFQVFDHSSIFLLIAGSYTPFCL
                     LSIRGWLGWTLFVLIWLFAILGIIYKALTLHKRERVSKVSTIIYIFMGWLCVVAAVPL
                     YHSLGMTGVILMVAGGLSYTIGAFFYSLNSIRYMHVVWHLFVMLGAGFMYFSVLFFT"
     gene            7021..7863
                     /locus_tag="SCJ86_001927"
     CDS             7021..7863
                     /locus_tag="SCJ86_001927"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DegV family protein"
                     /protein_id="HEG1169464.1"
                     /translation="MTNVKIVTDSSCTMEKNVRDALGIRVMPLSIMIDGTVYPDDDQL
                     DGERFMEMMAKAKNLPKTSQPPIGEFISMYDELGEDGSEVISIHMTKGLSGTVEAARQ
                     ASNLTKTKVTVIDSDTTDQGLSFQVIRAAELAKEGKTVEKILEEVKKVQDNTKLYIGI
                     STLDNLVKGGRISRTTGLLSSVLNIRVVMNFAHSELIPVTKGRGKKTFDKWFNNLKNE
                     LKELPNVRQIGISHADAKELAQEFKEGLQELFPDMHIPVLHTNPVIATHTGKDAFAIM
                     YYVD"
     gene            8021..8917
                     /locus_tag="SCJ86_001928"
     CDS             8021..8917
                     /locus_tag="SCJ86_001928"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294417.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SGNH/GDSL hydrolase family protein"
                     /protein_id="HEG1169465.1"
                     /translation="MKLTKRHLILVLFPLLIAIIIYAGLAASIPKAGPLMKPVTTVRE
                     DPNKKEKLHYIAIGDSLTEGVGDQTKQGGFVPLVANDLKDRYNLTAVETENYGVNGER
                     SDQILKRVKTKEEIRNNIKTADFITLTVGGNDLMKVIQNNLFGLTVNSFKKPLKNYQE
                     NVTELLEEFRSLNKTAPIYILGIYNPFYLNFPEITDMQTIVNNWNKGTETVTKKMPNC
                     YFIPINDLLYQGLNQEVGVTEEGTDQTTSTTENLNGIKNNVLYEEDHFHPNNLGYQLM
                     ANAVRDQLIETQNQWLEKEDDQ"
     gene            8920..9633
                     /locus_tag="SCJ86_001929"
     CDS             8920..9633
                     /locus_tag="SCJ86_001929"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288396.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YpmS family protein"
                     /protein_id="HEG1169466.1"
                     /translation="MKTRSDVSSNKTKKTNYWKFAFLILLGVVLGSTVFLGSRIFANR
                     EPELPEIPALTERQGDPVLTINSNKEKVNQIISFFLSEYQKDSDIEYKFYLENEALLN
                     GTFEVLGFPIDFYLYFDPYVMENGNVQLKARSLSIGTLSLPIKDVMNMIKRNYKLPDW
                     IEVNTDDLTIMLRLDQFRMQNGMYIKADKIDLVNDDIRFSLYLPKDTSDASNNSNTNK
                     TSTSSTESKTKENKKSSDE"
     gene            9653..10171
                     /gene="msrA"
                     /locus_tag="SCJ86_001930"
     CDS             9653..10171
                     /gene="msrA"
                     /locus_tag="SCJ86_001930"
                     /EC_number="1.8.4.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309572.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide-methionine (S)-S-oxide reductase MsrA"
                     /protein_id="HEG1169467.1"
                     /translation="MERAIFAGGCFWCMIQPFDTLPGIHTVMSGYTGGHVPDPTYEQV
                     KSQTTGHTEAVEIIYDPTLISYEQLVELYWQQTDPTDAFGQFEDRGDNYRPVIFYLTE
                     EQRIIAEKSKKQLAESGRFEQPIVTKIEPAQPFYLAEEDHQEYYKKHPENFKRNHARR
                     AAFIEENWRNEG"
     gene            10168..10395
                     /locus_tag="SCJ86_001931"
     CDS             10168..10395
                     /locus_tag="SCJ86_001931"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300423.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YozE family protein"
                     /protein_id="HEG1169468.1"
                     /translation="MNRSFFHYLMTLRGGGTHDALSVFATEAGKDIQFPKHSSSYEEI
                     SNYLELNVDYLPSMDIFDEAWEKYLENNHDI"
     gene            10547..11098
                     /gene="lepB"
                     /locus_tag="SCJ86_001932"
     CDS             10547..11098
                     /gene="lepB"
                     /locus_tag="SCJ86_001932"
                     /EC_number="3.4.21.89"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptidase I"
                     /protein_id="HEG1169469.1"
                     /translation="MTKKQRYINRFWLVFKYLLVSVFLAFMLRGFLFIPVPVEGNSME
                     NVLKQGDMVVMEKFSEIRRFDIVVFQLADGTIYIKRVIGLPGENVSYQNDQLKINGKV
                     VKEPYLTKNLKSDHANASYTTDFTLQELTGQSKLPEDNYFVLGDNRRVSKDSRSFGTI
                     NKTDILGKARFVYYPLDEIKWIQ"
     gene            complement(11181..11681)
                     /locus_tag="SCJ86_001933"
     CDS             complement(11181..11681)
                     /locus_tag="SCJ86_001933"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320949.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="folate family ECF transporter S component"
                     /protein_id="HEG1169470.1"
                     /translation="MNARLLSTSALLLALDVILYKIAIGPAYFQITFGFLSMALMGYL
                     YGPIWAGLLEMLSNTLNFTIFGSGTFQAAFLITAFLGGFINGLFLYHKKINLPSVIAA
                     QLLIMIPVSLFINSWLMSVLFGADFKVIFLGRFIRNMIQIPIQIMVVYSFIKTLEARK
                     VFKRAV"
     gene            complement(11960..12358)
                     /locus_tag="SCJ86_001934"
     CDS             complement(11960..12358)
                     /locus_tag="SCJ86_001934"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330224.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HEG1169471.1"
                     /translation="MNSMVFVNFPVADVNRSVAFYEKLGFKKNEEFSTEEAGSMVWDD
                     TFWIMLLSHDFYKKFIKDKEIADNKKTSGALISFSVENADEVKKFAQIAKENGGTFHE
                     VDMGMPEEEMFSLEVQDLDGNTLEPVWMKM"
     gene            12618..13478
                     /gene="ylqF"
                     /locus_tag="SCJ86_001935"
     CDS             12618..13478
                     /gene="ylqF"
                     /locus_tag="SCJ86_001935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324661.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome biogenesis GTPase YlqF"
                     /protein_id="HEG1169472.1"
                     /translation="MTIQWFPGHMAKARREVSEKMKYVDIVFELVDARLPLSSRNPML
                     DQIIQQKPRLVLLNKADLADPQQTKLWQEYFRKQGHLALAITAQESKGIKGLVPAAKE
                     ALKEKRARDAARGIKPRAIRAMVLGIPNVGKSTLMNRLVGKKIAQTGNKPGVTKGQQW
                     LRLGNELELLDTPGILWPKFEDEEIGKKLALTGAIKDQLVHLDDLAIYGLDFFARYYS
                     GRISDRYGLSIEQEEKLAPELLMDITAKRGYKEDYDRGSELVVHDIRQGKLGRYTLDR
                     CEEMEQKSDD"
     gene            13471..14238
                     /locus_tag="SCJ86_001936"
     CDS             13471..14238
                     /locus_tag="SCJ86_001936"
                     /EC_number="3.1.26.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008264725.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease HII"
                     /protein_id="HEG1169473.1"
                     /translation="MTESIQSIKERLKSIQRIDDPTLESLRNDPRKGVQQALASFERR
                     LKKEALIKEKYEEMFCFEKQGYAQGHQWIAGIDEVGRGPLAGPVVAAAVILPKNEIIL
                     GLNDSKQLSEKKRELLYQQVQEKALAIGIGIVDQQTIDDVNIYEASKLAMIQAVEKLS
                     IVPSYLLIDAMTLDLPIQQEKIIKGDARSVSIAAASIIAKVYRDQLMKEYDVLYPGYG
                     FSHNAGYGTKEHLEGLERQGITPIHRKSFAPVKKYLQ"
     gene            14294..15151
                     /gene="dprA"
                     /locus_tag="SCJ86_001937"
     CDS             14294..15151
                     /gene="dprA"
                     /locus_tag="SCJ86_001937"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309564.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-protecting protein DprA"
                     /protein_id="HEG1169474.1"
                     /translation="MYQIEENLLKLAYTKGISSQGKWALANWMMQYPYKEIGFEDIIK
                     IGKITKHRELFRKSWQEIHANWGKIQSKQSFITCFDPKYPPQLLHLTYPPIVLFYNGD
                     LSLLSCNMLSVVGGRHTSALAKKTVYYLLKPVIEAGYVIVSGCAKGIDTYGHQAAISH
                     GGRTIAVIGTGIEQVYPAENRDLQEVIANDHLLLSEYPPQSGPKKHCFPMRNRIIAAL
                     SKGTCVFEAKKKSGSLITAQQALDLGRTVFSVPGNILTEHSTGCHHLIQDGAVCTVSG
                     EDILAEFKE"
     gene            15273..17351
                     /gene="topA"
                     /locus_tag="SCJ86_001938"
     CDS             15273..17351
                     /gene="topA"
                     /locus_tag="SCJ86_001938"
                     /EC_number="5.6.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002380330.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I DNA topoisomerase"
                     /protein_id="HEG1169475.1"
                     /translation="MAYKYLVIVESPAKAKTIEKYLGRNYKVMASVGHIRDLPKSKMG
                     IDFENNYEPHYISIRGKGDVIKSLKAAAKKAQKVYLASDPDREGEAIAWHLAYLLGLD
                     LKEKNRVVFNEITKDAVKAAFKEPRTIDVDLVDAQQARRTLDRIVGYSISPILWRKVK
                     KGLSAGRVQSVALKIIIDREKEIREFVPEEYWSIDGNFKKARKKFKANFWGIDGKKKK
                     LPDAQSVKEVTARIDGTDFEVKKVEKKERKRNPANPFTTSSLQQEAARKLNFRTRKTM
                     MVAQQLYEGITLGKQGTVGLITYMRTDSTRIADTAKAEAAAFIEDTYGKEFSRHDHRK
                     IKNAQGAQDAHEAVRPTSVLRTPDELKQYLDKDQLKLYTLIWSRFVASQMTPAILDTM
                     KVTLEQNGVIFIANGSKIKFKGFMQVYVEGRDDGKEEKENILPELEEGDVVQSVDIEP
                     KQHFTQPPARFSEATLIRALEENGVGRPSTYAPTLDTIQRRYYVKLTQKRFEPTELGE
                     IVNSLICEFFPQIVDIHFTAEMEGDLDKIEEGTEAWVKVVDRFYKPFEKELTNAEEKI
                     EKIQIKDEPAGFDCDVCGHPMVIKLGKYGKFYACSNFPDCRNTKPIVKEIGVTCPVCH
                     EGQVIERKSKKNRLFYGCSRFPECEFTSWDKPVGRNCPKCDGYLVEKKVKGGKQVVCV
                     NGDYEEDIQK"
     gene            17320..18696
                     /gene="trmFO"
                     /locus_tag="SCJ86_001939"
     CDS             17320..18696
                     /gene="trmFO"
                     /locus_tag="SCJ86_001939"
                     /EC_number="2.1.1.74"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320953.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FADH(2)-oxidizing
                     methylenetetrahydrofolate--tRNA-(uracil(54)-C(5))-
                     methyltransferase TrmFO"
                     /protein_id="HEG1169476.1"
                     /translation="MEITKKTSKNNRKEVLSLKMVNVVGAGLAGSEAAWQIAQAGVPV
                     RLYEMRPVKSTEAHHTSNFAELVCSNSLRGNSLANAVGLLKEEMRRLNSVVITSADET
                     AVPAGGALAVDRDSFSETITRKVKEHPLVTVINEEITEIPEGITVVATGPLTSHPLAE
                     SIKAFNGSDGFYFYDAAAPIVDKATIDMDKVYLKSRYDKGEAAYLNCPMTEEEFKAFH
                     EALINAEVAELKSFEKEKYFEGCMPIEVMAQRGFKTMLFGPMKPVGLEDPKTGKRPYA
                     VIQLRQDNAAASLYNIVGFQTHLKWGEQKRVFRMIPGLENAEFVRYGVMHRNSFMNSP
                     ELLKPTYQSTKREDLFFAGQMTGVEGYVESAASGLLAGINAARLAKEMEPVEFPQETA
                     IGSMAYYITHAEGKHFQPMNANYGLFPELPERIRDKKARYEAIAQRALKANEAIKTEL
                     EKTAIPNE"
BASE COUNT         6518 a         3231 c         3899 g         5092 t
ORIGIN      
        1 ttttgtcgca gccccatttt tttgttgtga taaaagaaca agaaaggcag gaatgggaaa
       61 gtaaacatcc ttttgtcaga aacttttatc tttcttaagt tttacttgat atctgatact
      121 atttggaata gaatgaggcg ttgaaaaatt tatggaaatt tctaactagc aataaacgat
      181 gaaacaatga aatatttttc atccaaaaag aactttgcta taatgatgga agtttgtgta
      241 taggagatct ggaaaatgag agagagtttt gaacaacaaa aaaaactgtt gcatgatcgc
      301 tatggcgccc tttcgatgga tgaccgccgt caaatacttt gtaagttgcg taaaagaaat
      361 atattgatgt atcgtcagtt ggaacgtctg aaacatgatc tgcttcgttt ggagtcaaaa
      421 cgtgtacagt gtgaattaga ggggaaccaa acacaggttg aagtagtcga aaccaaaatt
      481 ttaaagaaaa aagaacaatt tttaaaaatg cttacacaaa ataaaaaata aaggggatgg
      541 acgatggaat ttgctgagtt agttattgta tttgcagtaa cgatcacctt atcgaacata
      601 gctagccgca ttttgccaat gattccagct ccactgatcc aaattttcct tggagtggta
      661 ttaggtctga cagagtgggg acaatcaatc gattttgaac ctgaattgtt cttagttatg
      721 attattgcac cattgttatt tagagaagga gaaaaagcag atatttcttc gattctcaaa
      781 aatttcggga caattttgtt cttggcattt ggaggagtca ttttgaccct tgtcggggta
      841 ggtgcgacgt tatcattcct gttgccaagt gtcccacttg ctgcctgctt tgcttttgga
      901 gcagcgttag gtccgacaga tgcggtagca gtcagttctc tttctggacg agtgaatatc
      961 cctaaaaagg cgatgcatat tttggaagga gaaggtcttt tgaatgatgc atctggtgtt
     1021 actgccttcc agtttgctct tggtgcgctg atcaccggat ctttttcagc agtaaacgcg
     1081 gggatgagtc tagtagtttc tagtattggg ggagctttga tcggcttttt gcttgtctgg
     1141 ttcaaacaaa aaataattca tttgatcgaa aaagcatcag cacaagatgt cacagggtat
     1201 ctgctgatcg aacttttgct tccttttcta gcctatgtat tggcagaggc atttgatgta
     1261 tctggaatca ttgctgcagt ggcagcaggt attctccaag cttctggttt taggaaaatc
     1321 tctgtgtttg atgcagaatt gtcgagcttg tcccacagta catggacaac tattgctttt
     1381 acactgaatg cattagtatt tatattttta ggaatcgaat tgacgcaagt gttctcgcca
     1441 gtatggggag acggtttata tccaaacggt cttttattag cgattatcgt attaatctct
     1501 gtcatgcttt ttgtgatcag attcatttct atcagtcttt tttatgtatt caaagatggc
     1561 agtaaaaagt tcaaaaaaca gttgaacgag atcttgatct taacatttgg cggtgtaaag
     1621 ggaacagtca gtttggctac gatttttatt ttgccatttt caatcaataa tatgatgttt
     1681 taccagcggt ctttgctttt gtttctgacg gcgggtgtca tcttagtgac tttggtcata
     1741 gggatcattg tcttgcctat gctgacggaa acagaggaag cgaaaagcac ggatttgaat
     1801 gctttgatga ttttagaaga ggtcgttgaa gttttgcgaa aagaaataaa ggaaattgat
     1861 cgtaatacaa aagaattttt agccactgaa gcagtgatag aaaattatca agaacggata
     1921 cgtgatctgt acttggaaga tttaaccgat gatgaaaagc aagaagtaca agaaatacaa
     1981 gcactgattt tgtcgatcga acgggatgga ttggatgaaa gttatcgttc gggcaagctt
     2041 tcttctaatg gttatcgttt ttactcccgt tttctttctc gattcgaaca ttctatcaca
     2101 agccagattt tgtcttttat cggcttttgg ttcattgttg tccgtaggtt aatgcgaatc
     2161 gttctccatc cgaaaatgtt ttggcaaaga agatatgctg atcgtcagac atttattagt
     2221 gatgaagata tccaagaaat cagagagaca tatcaaaaaa atactcagtt gattattgaa
     2281 agtttagata acttgactga tatttatgat gaaacgatgg ttcaattttt tatccagcaa
     2341 cgcaaacttg aaggaataaa aatgaactcg gggaatttga tttcttcgtg gatgatccag
     2401 caagatagtc tgtttacgaa aaaaatgcta agaggttatt atcttgaaag aaaaatcatt
     2461 gatgaatacg aagttgctga aaaaatcacg acgttttctg cgaacagcta tcgcaggaat
     2521 atcaatttgt tagaatctta tacgatgaac aaaccatctg ataacttttc tttccgattt
     2581 gcgtttcgag caaagtcaaa aaaacgagtc caataaaata aaaaggtaaa aattttcaag
     2641 ttttataaaa aacaaaacat atcagaacag gatcgaatcg gtcctgttct ttttttgaga
     2701 acacaaaaag aacaaatacc aaaaaaattt tcctgaagtt cactattttt atgatacaat
     2761 atccaagata aggagggaac atgttgaaag aattaaaagt aactgacctc aaaaaaactt
     2821 atggtgaaaa agatttgttt gaccagattt cttttttggt ccacgaaaaa gaccgtatcg
     2881 ggttgattgg gacgaacggg acgggtaaaa caagtctctt aaatattatt gccggtatcg
     2941 atagcagcga cggtgatcgg caaacagtgt tttatccaac tgattaccgt attggttatc
     3001 tttctcaagc agctgaattt tccgatgaat taactgttct ccaagcagtc tttcaaggaa
     3061 acagcccctt gatccaaact gttcgagcat atgaggaagc attgatcgaa ctaggggaaa
     3121 acggagaaga ccctgatgtg caaaaacgtt atgctaaagc agaagaacaa atgaataaag
     3181 aagatgcttg gactacagat accaacgcaa agattatttt gcaaaaatta ggaatcagtg
     3241 aattagataa aaaaatcagt actttatcag gtggtcagaa aaaacgagtt agtctggcgc
     3301 aagtgttgat tgatgaaccc gatttactat tactagacga accaacaaac catttagatt
     3361 atgaagcgat cgagtggttg gaaaactatt tgaaacaata ccgtggcgca ctattgatgg
     3421 ttacccacga tcgttatttt ctagatagag tagcaaaccg aatcttcgaa ctttcatttg
     3481 gaaaattata cgagtataaa ggaaattatg aagcttacgt cttagaaaaa gcagaacgtg
     3541 atcgtgtaga agtagaacaa gaagaaaagc gcaagcggct ttataaacag gaattagctt
     3601 ggatgagggc tggcgtacag gcacgtggca ccaagcagca agcacggatc aaccgtttcg
     3661 aagatctaaa ggagaacttg taccaagtaa accaagaaga tgatttagag ctgaacctag
     3721 cgacacaacg tttagggaaa aaagtactgg aaatcaaaga tggcagttac cgtataaatg
     3781 accaaacact gctagaacat ttagatttat tgattcaatc tcgggaaaga ctaggtatta
     3841 ctggtaaaaa tggtgcggga aaatcgacat tgcttaatat tctagctggc agaattcccc
     3901 tggatagcgg tacaatgtct atcggtgaaa cggttcatct tgcgtattat acacaagaaa
     3961 atgaagaaat ggcaccagat aaacggatga ttgcttactt acaagaagct gctgaagaag
     4021 caaaaacagc agatggcagt caaatcggag tagcagaatt acttgaacga tttttatttc
     4081 caagatttat gcatgggaca ctgataggca aactttcagg cggagaaaaa cgccggttat
     4141 ttttgttgaa actgctgatt cagcaaccta atgttttatt attggatgaa ccgaccaatg
     4201 atttggacat agcaacattg acgattttgg aagattattt tagaagtttt ccaggtgctg
     4261 tgatcactgt ttcccatgac cgttatttct tggacaaggt agcagataag ttattagtat
     4321 tccaaggaaa cggaaaacaa gaactttatt acggaaacat gtccagttat ttactgaagc
     4381 aaaaagagac ccagcagcct gctgaaaaag cgaaaccaaa aatacagagc aaggaacctg
     4441 ctgaaaagaa gaaattatct tacatggaac aaaaagaatg ggaaacgatt gaagatgaga
     4501 ttgcagaact tgaagaaaaa atctcattgc tccaagaaga gatgaatcat caaggagata
     4561 atttcactcg attacaagaa ttgcaaaacg acgtatctga aacagaagca caacttgaag
     4621 aaaaaatggc acggtgggaa tacttgagtg aatgggtaga agattaggag gaaaaaggat
     4681 ggaacaagcg tatcttgact taggaagaaa attgttggaa gaagggcatc gtaaagaaga
     4741 cagaacaggc acaggcacat taagcttatt cggataccaa atgcggtttg atctgcaaaa
     4801 agggtttcct ttgttaacaa cgaaacgagt accttttgga ttgatcaaaa gcgaattgct
     4861 ttggttttta aaaggagata cgaatatccg ctatctatta caaaataaaa accatatttg
     4921 ggatgaatgg gcatttgaac gctacatcaa aagtccagac tataatggac cagatatgac
     4981 tgattttggc catagatgtt tgacagatcc tgaattcaat gaagaatata aaaagcagag
     5041 caagcttttt tgcgagaaga tcctaaccga tgattccttt gctgaaacat acggcgatct
     5101 tggacacatc tacggctatc aatggcgtca ctgggaaaca aaagacggtg gctttatcga
     5161 tcaaatcaaa caagtgatcg aagcaatcaa aaagactcct gattctagaa gattgatcgt
     5221 ttctgcatgg aatccggaag atgttcctag catggctttg cctccttgtc atacgatgtt
     5281 tcaattttat gttcaagagg gacggctaag ctgccaattg tatcaacgca gtggggatgt
     5341 gtttctaggc gttccgttca acattgcaag ttatgcgttg ctgactcacc taattgcaca
     5401 tgaaacaggt ttagcggttg gcgaatttgt tcacacatta ggagatgctc atctttatat
     5461 caatcatatc gaacaaatga aagaacaatt gagccgagaa atacgcgcat ttccgacttt
     5521 agttttgaat caggaaaagc agtcagtatt tgattttgag atggaagata tccaattaga
     5581 aggatatgac ccacatccgg cgatcaaagc tcctatcgct gtttagaatc agaaaggatg
     5641 agaaaatgtt agctgcaatt tgggcacagg atgaaaacgg attgattgga aaagaagacc
     5701 gtcttccttg gcgtttgcca aatgatctga aattcttcaa acaaatgaca gaagcaaata
     5761 cactagtgat gggacgaaaa acattcgaag ggatgggtag ccgtccgttg ccgaatcgtc
     5821 agacgattgt tttgacgaga gattcttcct ataaagcaga aggcgtgcat gtcatgcata
     5881 gtgtggaaga cgtgcttgct ctggaaaaag aaacagacgg tatctttttc atcgctggag
     5941 ggtcagctgt ttatcaagaa tttctgccat tttgtacgat attataccgt acagtcatcc
     6001 atcatgtatt cgatggagat gcttattttc cacctgttga ctggtctgat tggtcactga
     6061 tcaatactag tcaaggagaa atggatgaaa aaaatcgtta tccgcatcaa tttgaaacgt
     6121 atcagcgtaa tgagaatcct gtaaaaagcg ggacaggcga attgacgata taaaaaagct
     6181 atggatagac tagctgttat ttagagctgt cccatccata gctttatttt ttttattaag
     6241 taaagaataa aacagagaag tacataaaac ctgcacctaa cataacaaac aaatgccaaa
     6301 caacatgcat atagcggata gaattcaagc tataaaagaa tgcgcctatc gtgtaagaaa
     6361 gcccgcctgc taccatcaaa ataacaccag tcattccaag agagtgataa agaggaacag
     6421 cagccacgac acagagccac cccataaaaa tatagatgat cgtagatact tttgatactc
     6481 tctcccgttt atgcaacgtc aacgctttat atataatgcc aagaatcgca aacaaccaga
     6541 ttaatacaaa taaagtccaa cctagccatc ctcggatact cagcaaacaa aaaggagtat
     6601 aacttcctgc aatcaaaaga aaaatcgaac tatgatcgaa tacttggaac accttttttg
     6661 cttttgtaaa aatcaatgaa tgaaacaaag tagacgttaa aaataaaaga atcatcatcg
     6721 atccatagat agcataagag acaatatgga gggaggaatg caagtgggca ccttttacta
     6781 aaagaaccac tagtcccgcg atactcaatc cagtcccaat tccatgggtg actgcattaa
     6841 acacttcatt taatatttca tacgttcgat taaatttcgt tttttccatg aaaacggctc
     6901 ctttactagg tgtaattaat gaaaaatcat tatttgtctg ttatactaaa aatagaaatt
     6961 gtcttacatg tagtttaaca tgaataaggc atcttgttaa agaaagagag agagaaaata
     7021 atgacaaatg taaaaatcgt aacagattct tcatgtacga tggaaaaaaa tgtacgcgat
     7081 gctttaggca ttcgtgtcat gccgctatcc atcatgatcg acggaacagt atatcccgat
     7141 gatgaccaac tagacggcga aagatttatg gaaatgatgg cgaaagcaaa aaatctgccc
     7201 aaaacaagcc aaccaccaat tggagaattc atctcgatgt atgatgagct aggagaagac
     7261 ggcagtgaag tgatttcgat ccatatgaca aaaggcttga gtggaacagt agaagcagca
     7321 agacaagcca gtaatctaac aaaaacgaaa gtaacggtta tcgacagtga taccactgac
     7381 caaggattat cttttcaggt aattcgagca gctgaattag caaaagaagg taaaacagta
     7441 gagaaaatcc ttgaagaagt caaaaaagta caagacaata caaaattata catcggaata
     7501 tccacactag ataacttggt aaaaggtgga agaatcagcc gaacaactgg tttgctgtct
     7561 agtgttttga acatacgcgt ggtgatgaat ttcgcccatt ctgaattaat ccctgtaact
     7621 aaggggcgag gaaagaaaac gttcgataaa tggtttaata atttgaaaaa tgaactgaaa
     7681 gaattgccaa atgtgcgaca aatcggtatt tcacatgcgg atgcaaaaga attggcgcaa
     7741 gaattcaaag agggtttgca agaattattc ccggacatgc atatccctgt actgcatacg
     7801 aatccggtta tcgctacgca tactggtaaa gacgcgtttg caatcatgta ttatgtagat
     7861 tagtcgaaaa gaggataagc cctcagcctg aaaggcttgc agcttattct ctttttcttt
     7921 ataaaattag gaaaataatt gttttctttt ttcataaaga ctaccttttt gataaaattt
     7981 ttgataacat tagaaccaat cgaatacaag aggtgaaaaa atgaaattaa cgaaacgaca
     8041 tttgatcctc gttttgttcc cattattaat tgcgatcatc atctatgcag gtttagctgc
     8101 ttctataccg aaagctggcc cactaatgaa accagtaact actgtgagag aagatccaaa
     8161 taaaaaagaa aaacttcact acatagctat cggagattcg ctgactgaag gagtaggaga
     8221 tcaaaccaag caaggaggat tcgttcccct tgtcgcaaat gatctaaaag accgttataa
     8281 tttgaccgcg gttgaaacag aaaactatgg tgtaaatggt gaacgaagtg accagatctt
     8341 aaagcgagta aaaacaaaag aagaaattcg aaacaatatc aaaacagctg attttatcac
     8401 actgactgtt ggcggtaatg atttgatgaa agttatccaa aataatttat tcgggttaac
     8461 ggtcaattcc tttaaaaagc cactgaaaaa ctatcaggaa aatgtcacag aactattgga
     8521 agaatttcga tccctcaata aaaccgcacc tatttacatt ttaggcatct acaatccatt
     8581 ttatttgaac tttcctgaga tcactgacat gcagacaatt gttaacaatt ggaacaaagg
     8641 aacagaaacg gtgacaaaaa aaatgccgaa ctgctacttt atcccaatca atgatttgct
     8701 ttatcaagga ttgaatcagg aagtaggtgt cacagaagaa ggaacagatc aaacaactag
     8761 cacgacagaa aatctaaatg gcatcaaaaa taatgtgctc tatgaagaag atcatttcca
     8821 tccaaataat ttaggttatc aattgatggc aaatgcagtg agagatcagc tgatcgagac
     8881 acaaaaccaa tggttagaga aggaggatga tcaataaaca tgaaaacaag atctgacgta
     8941 tcttcaaaca aaacaaagaa aacaaattac tggaaattcg ccttccttat ccttcttggg
     9001 gtagtacttg gttcgaccgt atttttaggc agccgtattt ttgctaatcg ggaaccagaa
     9061 ctcccagaaa taccagctct tacggaaaga caaggagatc ctgtcttaac gatcaattca
     9121 aataaagaaa aagtcaatca aatcatcagc tttttcctta gtgagtatca aaaagatagt
     9181 gatattgaat ataaatttta tttggaaaat gaagcactac tcaatggtac tttcgaagta
     9241 ctaggttttc ctatcgattt ctatttatat tttgatccgt atgtgatgga aaacggaaat
     9301 gtccaactaa aagctagaag tctttctatc ggaacattga gtttgccaat caaagatgtg
     9361 atgaatatga tcaagagaaa ctataaatta ccagattgga tcgaagtcaa tacagatgat
     9421 ctgactatca tgcttaggtt ggatcaattc cgaatgcaaa acgggatgta tattaaagca
     9481 gataaaatcg acttagtaaa tgatgacatt cggtttagtc tatatttgcc aaaagatacc
     9541 tctgatgctt caaacaactc aaataccaac aaaactagca ccagcagtac tgaaagcaag
     9601 acaaaagaaa ataaaaaatc atcggatgag tagtattaga gggggaatca taatggaacg
     9661 agcaatattt gcaggaggct gcttctggtg tatgatccag cctttcgaca cgcttcctgg
     9721 tatccataca gtgatgtcag gatatactgg agggcatgta ccagatccga cttatgaaca
     9781 agtaaaaagc caaacgactg gacatacaga agcagtggag attatctatg atccaacatt
     9841 gatttcatat gaacaattag tagaacttta ctggcagcaa actgatccaa ctgacgcttt
     9901 cggacaattc gaggatcgcg gtgacaatta tcgcccggtg atcttttatt tgacagaaga
     9961 acagcggatc attgctgaaa aaagtaagaa acagttagct gaaagtggac gtttcgaaca
    10021 gccaatcgtt acaaaaatcg aaccagccca accattttat ctagcagaag aagaccatca
    10081 agagtattat aaaaagcatc ctgaaaattt taaaagaaac catgcacgcc gggctgcctt
    10141 tatcgaagaa aattggagga acgaagggtg aatcgaagct tttttcatta tttaatgaca
    10201 ctgcgaggcg gtggcacaca tgatgcttta tctgttttcg ctacagaagc cggtaaagac
    10261 atccaatttc ctaagcacag ctctagctac gaagaaattt ctaattatct ggaactgaat
    10321 gtcgactatt tgccgtccat ggatatcttt gacgaagcat gggaaaaata tttagaaaat
    10381 aatcatgaca tataagagat agttttgtac tttatgatca aaaaagtttt ttgtccaata
    10441 gcctaattga aagaattatt tttatttcag ctatctagtc gcattttttt gatatttttc
    10501 ttataataaa gaaagaatga aaaaaagagg gatggattga tagagattga caaaaaaaca
    10561 acgctatatt aatcgcttct ggcttgtatt caaatactta cttgtttctg tattccttgc
    10621 gttcatgttg cggggatttt tattcatccc ggtaccagtg gaaggcaatt caatggaaaa
    10681 cgtgttgaag caaggtgata tggttgtgat ggaaaaattt tcggagatca gacgatttga
    10741 tattgttgtt tttcaattag cagatggcac gatttatatc aaacgagtaa tcggtcttcc
    10801 aggagaaaat gtcagctacc aaaatgatca attgaagatc aatgggaaag tagttaagga
    10861 accttatcta actaaaaacc tgaaatcaga tcatgcaaat gcatcctata cgacggactt
    10921 tacactacaa gagctaacgg gacaatccaa gcttccggaa gataattatt ttgtacttgg
    10981 agataatcga agagtttcaa aagatagtcg ttcgtttggc acgatcaata aaactgatat
    11041 tctaggtaaa gcaagatttg tctattatcc acttgatgaa ataaaatgga tccagtgaga
    11101 aacaataaag cgtgcagtct gttgagactg cacgctttat tgtttaatcc aaaatcagtt
    11161 tactacctta gtacgtaaaa tcaaacagct cgtttaaaca cttttctagc ctctaaagtt
    11221 ttgataaatg aataaacaac cattatttga atagggattt ggatcatatt cctgatgaat
    11281 ctacctagaa aaattacttt gaaatctgca ccaaacaaaa cagacatcag ccaagaattg
    11341 ataaaaaggc tgactgggat cataatcaat aattgggcag cgatgacgga aggcaggttt
    11401 atctttttat gatataaaaa taagccatta ataaatccac caaggaaagc cgtgataaga
    11461 aaagcagctt gaaacgttcc tgaaccaaat atcgtgaaat ttaaagtgtt cgacaacatt
    11521 tcaagcaaac cagcccagat cggtccatat agataaccca taagcgccat acttaaaaat
    11581 ccgaatgtga tttgaaaata agcaggacca atcgctatct tgtataagat gacatcaagg
    11641 gctaataaaa gagcagaggt cgacaacaat cttgcattca tttcgggcaa tctaaaaacg
    11701 tcttttgcaa tcaaagtgtt caaaatattc tctcctttgt cagttaataa taaaaggata
    11761 ccataaaatc accaatgtgt catgacacat tggtgattta catacactgg catctaacaa
    11821 ttaaaaaaag ggtgagaaga ctgcttttat aaactgtaaa aaagagattg tacgaataaa
    11881 aaaaatagag gcggagaata cttatcgtat catccgcctt tgtttttttg ttataaaaaa
    11941 cgtcagatta gttatctgct tacatcttca tccatacggg ttctaatgta tttccgtcta
    12001 gatcttggac ttctaatgaa aacatttctt cttcaggcat tcccatatct acttcgtgga
    12061 aagtaccacc attttctttt gcaatttgag caaatttttt tacttcatcc gcgttttcaa
    12121 cactaaacga aatcaaagca ccacttgttt ttttattatc agctatttct ttatctttta
    12181 taaatttttt ataaaaatca tgagagagaa gcattatcca aaaagtgtca tcccatacca
    12241 ttgaaccagc ttcttcagta gaaaattctt catttttttt aaaacccagc ttttcataaa
    12301 aagcaacgga acgattgaca tcagcaacag gaaaattaac aaaaaccata ctattcattt
    12361 gtttcgtctc ctttctgttt tgttcgtcta tcatctctta tttaggaaac gctgtcaaaa
    12421 atttagattc atatatagaa accaaaataa aaatttgcat catcaaaaaa gtgaagatgt
    12481 gatagaatga ggactggttc actttcactt aactaataga tggaatttct ttataataaa
    12541 aaagtgaatt tatattgaaa aagaaaaaaa caagtaaagc cggggaatat tccggtatga
    12601 aaggaatgga gagaacaatg accatccaat ggtttccggg acatatggca aaagcccgac
    12661 gagaagtttc tgaaaaaatg aaatatgtgg acatcgtatt tgaattggta gacgcacgat
    12721 tacctttatc ttctcgtaat ccaatgctag atcagatcat tcagcaaaaa cctcgccttg
    12781 ttttattgaa taaagcggat ctggcagatc cacaacagac aaagctgtgg caagaatatt
    12841 ttcgtaaaca aggccatctt gcattagcta tcacggcaca agaaagtaaa ggaattaaag
    12901 gtctagtacc tgcggctaaa gaagcattga aagaaaaacg ggcaagagat gctgctcggg
    12961 ggatcaagcc tcgcgctatt cgggcaatgg tcttaggtat tccaaatgtt gggaaatcta
    13021 cgttgatgaa tcgactagta ggcaagaaaa ttgcacagac aggaaataag cctggcgtaa
    13081 caaaaggtca gcagtggctt cgtttaggta atgagttgga attgttagac acaccaggga
    13141 ttttatggcc caaatttgaa gatgaagaaa tcggcaaaaa actagcgctg acaggcgcga
    13201 tcaaagacca gcttgtccat ttagatgatt tagcgattta cggattagac ttttttgccc
    13261 gatattattc aggaagaatc agcgatcgtt atggtttatc aatcgagcaa gaagaaaaat
    13321 tggcaccgga attactgatg gatattacag caaaacgtgg gtataaagaa gactatgatc
    13381 gtggcagcga attagtcgtg catgatattc gtcaaggaaa acttggacga tacacactgg
    13441 atcgttgcga agaaatggag caaaaaagtg atgactgaat ccatccaaag tatcaaagaa
    13501 cgattgaaaa gcatacagag aatagatgat cctacactag aatcattaag aaatgatcca
    13561 cgcaaaggcg tgcagcaagc actggcttct tttgaacgac gattgaaaaa ggaagcatta
    13621 attaaggaaa aatatgaaga aatgttttgc tttgaaaagc aagggtacgc acaaggtcac
    13681 caatggattg ccggcattga tgaagtagga agaggaccat tagctggacc tgtcgtggca
    13741 gctgcagtta tcttgccgaa aaatgagatc atattagggc tgaatgactc caaacaatta
    13801 tcagagaaaa agcgagaact tctttatcaa caagttcaag aaaaagccct ggcaatcggg
    13861 atcgggatcg ttgatcagca gacaatcgat gatgtgaata tttatgaagc aagtaaatta
    13921 gcgatgatcc aagcagtaga aaaattgtcc attgtaccaa gctatttgtt gatcgatgcg
    13981 atgaccttgg atttgcctat tcagcaggaa aaaataatca aaggggatgc acgatctgtg
    14041 tcgattgctg ctgcaagtat cattgctaaa gtatatcgag accaattgat gaaagaatat
    14101 gacgtgcttt atcctggata tggtttctcg cataatgcag gttatggaac aaaggaacac
    14161 ttagaaggac ttgaacgaca aggaattacg ccaatccatc gtaaaagttt tgcacctgta
    14221 aaaaaatatt tacaataaaa aacagccgct tttacgtatc ttattagaaa gagagacgaa
    14281 aggcggtttt atgatgtatc aaatagaaga aaatttattg aaacttgctt atacaaaagg
    14341 aatcagtagt caaggaaaat gggcacttgc taactggatg atgcagtatc cttataaaga
    14401 aatcggattt gaagacatca ttaagatagg taaaataacc aaacatcggg agctgtttcg
    14461 caaaagttgg caggaaattc acgctaactg gggcaaaatc caatcgaaac agtcgtttat
    14521 cacttgcttt gatcccaaat atccccctca gctgcttcac ttgacctatc cgccaattgt
    14581 actgttttat aatggtgatc tatctttact ttcttgtaac atgttaagcg ttgttggtgg
    14641 aagacacact tctgctttag ccaaaaaaac agtttattat ttactaaaac cagtgataga
    14701 agctggatat gtgatcgtca gtggctgcgc gaaagggata gatacatacg gtcaccaagc
    14761 agcaatctct catggtggac ggacaatcgc agtcattgga acagggatag agcaagtcta
    14821 tcctgctgaa aatcgtgact tgcaagaggt gattgcaaat gatcatcttc ttttgagtga
    14881 atatcctcct caatccggcc cgaaaaagca ttgttttcca atgagaaatc ggattatcgc
    14941 agctttgtct aaaggaacat gtgtctttga agccaagaaa aaaagcggct ctctgataac
    15001 agcacagcaa gcattggatc ttggaagaac agttttttct gttccaggaa atattttaac
    15061 agaacattct accggatgtc atcatttgat ccaagacggt gcagtttgca cggtttcggg
    15121 tgaagatatc ttggcagaat ttaaagaata acaccatact tttcatttgc ttttcttgac
    15181 aaacgttttc tgaatggata tgattagcta cgatttgtat gaatcctctt ttaatagtag
    15241 gaacacgaca atggaaagga gtccattcag taatggcgta taaatattta gtgattgtag
    15301 aatcacctgc aaaagccaag acaattgaga aatatcttgg acgaaactat aaagtaatgg
    15361 ccagtgttgg ccatataaga gatttaccga aaagtaaaat gggtatcgat tttgaaaaca
    15421 attatgaacc ccattatatt tctatacgcg gaaaaggcga tgtcatcaaa agcctgaaag
    15481 ccgcagcaaa aaaagctcaa aaagtttact tggcaagtga cccggataga gaaggagaag
    15541 cgattgcttg gcatttagcg tacctacttg ggttggatct gaaagaaaaa aatcgggtgg
    15601 tcttcaatga aatcacaaaa gacgcagtca aagcagcttt taaggaacca agaacgatcg
    15661 atgtagattt agtagatgca cagcaagctc gtcgtacctt agacagaatc gttggttatt
    15721 cgatcagtcc tattctctgg cgtaaggtca agaaagggtt aagtgcagga cgtgtccaat
    15781 ctgtcgcttt aaaaattatt attgaccgtg aaaaagagat ccgagaattt gttccagaag
    15841 aatattggag catcgacggt aattttaaaa aagctcgcaa gaaattcaaa gcaaatttct
    15901 ggggaatcga cggtaagaaa aagaaattac cagatgcaca aagtgtaaaa gaagtcactg
    15961 ctagaattga tggaacagac ttcgaagtta aaaaagtaga aaagaaagag cgcaaaagaa
    16021 accctgcgaa tcctttcact acaagtagcc tgcaacaaga agctgcccgt aagttaaact
    16081 ttcgaacaag aaaaaccatg atggtagcac agcaattata tgaaggaatc actttgggta
    16141 aacaaggaac agtcggcttg attacataca tgcgtactga ttctacccgg attgccgata
    16201 cagcaaaagc agaggccgca gcatttattg aagatacgta tggaaaagaa ttttctagac
    16261 atgatcaccg aaagatcaaa aatgctcaag gcgcacaaga tgcccatgaa gctgtgcgtc
    16321 caacgagtgt gcttcgtaca ccagatgaac tcaaacaata tttagataaa gatcaattga
    16381 aactttatac attgatctgg tcacgttttg tggctagtca gatgacacca gctattttag
    16441 atacaatgaa agtgacatta gaacaaaatg gtgtcatctt cattgcgaac ggttcaaaaa
    16501 tcaaattcaa aggattcatg caagtgtatg tggaaggtag agatgacggc aaagaagaga
    16561 aagaaaatat tcttccagaa cttgaagaag gcgatgtagt ccaatctgtt gatattgaac
    16621 caaaacaaca ttttactcaa ccaccagcac gttttagtga ggctacgttg attcgtgcac
    16681 tagaagaaaa cggggtaggg cgaccttcca cttatgcacc aacacttgat acgatccaaa
    16741 gacgctacta tgtaaaattg acacaaaaaa gatttgagcc gactgaacta ggagaaattg
    16801 tcaattcatt aatctgtgaa ttcttccctc aaatcgtcga tatccatttc acagcagaaa
    16861 tggaaggaga tttggataaa attgaagaag gcacggaagc gtgggtcaaa gtggttgatc
    16921 gtttctataa accgtttgaa aaagaactga cgaatgcgga agaaaaaatc gaaaaaattc
    16981 aaatcaaaga tgaaccagca ggattcgatt gtgatgtctg tggacatccg atggtgataa
    17041 aattagggaa atatgggaag ttttatgctt gcagtaattt tcctgattgt cggaatacga
    17101 aaccaatcgt aaaagaaatc ggtgtgactt gcccagtatg tcatgaagga caagtcattg
    17161 aacgaaaatc taagaaaaac agactattct atggttgttc ccgtttccca gaatgtgaat
    17221 tcacttcttg ggataaacca gtgggcagaa attgtccaaa atgtgacgga tatctggtcg
    17281 agaagaaagt caaaggcggt aaacaagtag tctgtgtaaa tggagattac gaagaagaca
    17341 tccaaaaata atagaaaaga ggttctttca ttgaaaatgg taaatgttgt tggagcaggg
    17401 cttgccggaa gtgaagctgc ttggcagatc gcacaagcag gagtccctgt ccgtttatat
    17461 gaaatgcgtc cagtcaaatc gactgaggcg catcacacaa gtaattttgc tgaactagta
    17521 tgttccaatt cattaagagg aaacagtttg gctaatgcag tagggttatt gaaagaagaa
    17581 atgcgccgat tgaattcggt agtaatcact tcggcagatg aaacagcagt gccagctggt
    17641 ggtgccttag ctgttgaccg cgattctttt tctgaaacaa ttacccgaaa agtcaaagag
    17701 catccattag taacagtgat aaacgaagaa attactgaaa taccggaagg aatcaccgta
    17761 gtagcaacag gacctttgac ttctcatcca ttagcagaaa gcatcaaagc attcaatggt
    17821 tcagatggtt tttatttcta tgatgcagct gcaccaatcg tagacaaagc tacgatcgat
    17881 atggataaag tatatttgaa gtcccgctat gacaaaggag aagcagctta cttgaactgt
    17941 ccgatgacag aagaggaatt caaagctttc catgaggcac taatcaatgc cgaagtggca
    18001 gaacttaaat cttttgagaa agaaaaatat tttgaaggat gtatgccaat tgaagtaatg
    18061 gcacagcgag gattcaaaac gatgcttttc ggacctatga aaccagttgg attggaagat
    18121 cctaaaactg gcaaacgtcc ttatgctgtt atccagctgc gtcaagacaa tgctgcagct
    18181 tctttataca acatcgtggg ctttcagact cacttgaaat ggggcgagca aaaacgtgtt
    18241 ttccggatga ttcctgggtt agaaaatgct gaattcgttc gctatggtgt gatgcaccga
    18301 aacagcttta tgaattcgcc agaattgctg aaaccgactt accaatcaac gaaacgcgaa
    18361 gatctgttct ttgcaggtca gatgactggt gtagaaggct atgttgaaag tgcagcgagc
    18421 ggactacttg ctggaatcaa cgccgcacgc ttagcaaaag aaatggaacc agtcgagttt
    18481 ccacaagaaa cagctattgg aagtatggct tactacatta ctcatgcgga aggaaaacat
    18541 ttccaaccga tgaatgccaa ctatggtctg tttcctgaac tgccagagcg cattcgagac
    18601 aaaaaagcaa gatatgaagc aattgcacaa cgagctttaa aagctaatga ggcaataaaa
    18661 acagaattag aaaaaactgc aatcccaaat gagtaaggaa actccatgaa gagggtgaga
    18721 cggatagtct cgctcttttt
//
LOCUS       DARBET010000052        18279 bp    DNA     linear   BCT 07-NOV-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4027
            SAMEA114569841-rid20112183.denovo.053, whole genome shotgun
            sequence.
ACCESSION   DARBET010000052 DARBET010000000
VERSION     DARBET010000052.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA114569841
            Sequence Read Archive: ERR12203666
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 18279)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 18279)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001972618.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001972618.1
            Genome Coverage       :: 132x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/07/2023 13:18:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,041
            CDSs (total)                      :: 2,975
            Genes (coding)                    :: 2,908
            CDSs (with protein)               :: 2,908
            Genes (RNA)                       :: 66
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 67
            CDSs (without protein)            :: 67
            Pseudo Genes (ambiguous residues) :: 0 of 67
            Pseudo Genes (frameshifted)       :: 26 of 67
            Pseudo Genes (incomplete)         :: 28 of 67
            Pseudo Genes (internal stop)      :: 20 of 67
            Pseudo Genes (multiple problems)  :: 6 of 67
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18279
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA114569841-rid20112183.denovo.053"
                     /isolate="VRE4027"
                     /db_xref="taxon:1352"
                     /country="Denmark"
     gene            complement(17..1246)
                     /gene="hflX"
                     /locus_tag="SCJ86_001940"
     CDS             complement(17..1246)
                     /gene="hflX"
                     /locus_tag="SCJ86_001940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324580.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase HflX"
                     /protein_id="HEG1169477.1"
                     /translation="MEEKVIIVGVETEANQRYFSESMEELVQLTNTASGEVVFTITQK
                     RPQVDRQTIIGKGKLQELVQQADAHEADLIIFNHELTPRQSQLITDAVGLPVIDRVQL
                     ILDIFAMRARSKEGKLQVELAQLEYLLPRLVGQGKTLSRLGGGIGTRGPGETKLETDR
                     RHIRNKIFAVKKELKEVEAHRERNRQKRKNSEIFQIGLIGYTNAGKSTILNLLTQADT
                     YSKDQLFATLDPLTKRWRFAEGFEITVTDTVGFIQDLPTQLIDAFHSTLEESQNMDLL
                     LHVVDASSPDRILQEQTVLKLMDELNMKEMPILTVYNKADQIDPAMFTPTLFPNVLIS
                     TQTKEGKMALIEAIKRQLMELMTPYTLSVPSNEGQKLSELRRQTMVLDEAYMEETNEY
                     EVKGFAKTNSKWLRKNQ"
     gene            complement(1248..2165)
                     /gene="miaA"
                     /locus_tag="SCJ86_001941"
     CDS             complement(1248..2165)
                     /gene="miaA"
                     /locus_tag="SCJ86_001941"
                     /EC_number="2.5.1.75"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002391642.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (adenosine(37)-N6)-dimethylallyltransferase
                     MiaA"
                     /protein_id="HEG1169478.1"
                     /translation="MEKVLVIVGPTAVGKTALSVELAQLFGGEIISGDSLQIYKNLDI
                     GTAKIKEEEMNNVPHHLIDVIEPTQRYSAADFQHSGRQLITEIANRGHLPIIAGGTGL
                     YIQSLLYDYQLGATEEANPEIRKKYETLAETIGSQALWNQLKEKDPAAAEKIHWNNQR
                     KVIRALEVLETTGHSITAPKEEPKQLYDYLMIGLDTKRSILYERINQRVDLMLAEGLV
                     AEARKVYELGEVQASQGIGYKEFFPYFEGKETLEEATEQVKLHSRRYAKRQLTWFRNR
                     LDAQWFDLISEPNQKSQIEQTIKKWLEAE"
     gene            complement(2169..2906)
                     /locus_tag="SCJ86_001942"
     CDS             complement(2169..2906)
                     /locus_tag="SCJ86_001942"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628886.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerophosphodiester phosphodiesterase"
                     /protein_id="HEG1169479.1"
                     /translation="MLQIYAHRGSSGTHPENTLPAFAEAVRVGADGIELDVHLSKDGY
                     LIVMHDEEVDRTTNGKGLIREKTLEELKKLNAGGKFSKEFPAAKVPELREVLGLLLQK
                     NYRGMLMIEIKTDQYEYPGIEEKLAETLKSRDWPFQHAYCSFHLDSLEKIALLEPEIQ
                     LDLLMSTSDKKIQLAAETPYIEGVHPKNTWLFEHPVQAADFPKAIRAWTINDEEEMAK
                     CQELKIDGIITDFPEKALRWKQSTKSR"
     gene            complement(2997..3524)
                     /locus_tag="SCJ86_001943"
     CDS             complement(2997..3524)
                     /locus_tag="SCJ86_001943"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010752644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEG1169480.1"
                     /translation="MIVGITGLIGVLTGAVVVYIGMILQMRHLENKEQRRRELELKVK
                     EIETLNQLNKKINEILQKRTTLMEEYVSFDAFDDCYITIDDFAYLQSFSAQNSFYLPN
                     YILDELFKNISHRKVILSPDETMKIGGYAYKGGRVILENFTDSLTEMINEKKAQLKTM
                     TRQPIGFFSKDDVWS"
     gene            3704..4297
                     /gene="clpP"
                     /locus_tag="SCJ86_001944"
     CDS             3704..4297
                     /gene="clpP"
                     /locus_tag="SCJ86_001944"
                     /EC_number="3.4.21.92"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp endopeptidase proteolytic
                     subunit ClpP"
                     /protein_id="HEG1169481.1"
                     /translation="MNLIPTVIEQSSRGERAYDIYSRLLKDRIIMLSGQVTDDLANSI
                     IAQLLFLDAQDSEKDIYLYINSPGGSVTAGMAIYDTMNFVKADVQTIVMGMAASMGSF
                     LLTAGTKGKRFALPNAEIMIHQPLGGAQGQATEIEIAARHILQTRERLNKILAERTGQ
                     PLEVIEKDTDRDNYMTAEQAKAYGLIDEVMENSSSLN"
     gene            complement(4401..4886)
                     /locus_tag="SCJ86_001945"
     CDS             complement(4401..4886)
                     /locus_tag="SCJ86_001945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008267279.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gamma carbonic anhydrase family protein"
                     /protein_id="HEG1169482.1"
                     /translation="MERFIASNATVIGDVTLSEDVTIWYQAVLRGDSNWIKIGQRTNI
                     QDGTIIHVDHDAPVDIAENVTVGHQCMLHGCTIEKGALIGMGTTILNHAVIGENSLIG
                     AGSLVTEGKVIPPNVLAFGRPAKVIRPLTKEEIQKNKENIQHYVEIGRMHAQGKFKEQ
                     R"
     gene            complement(5039..5236)
                     /locus_tag="SCJ86_001946"
     CDS             complement(5039..5236)
                     /locus_tag="SCJ86_001946"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296291.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEG1169483.1"
                     /translation="MEQFLNEKITEAKREVDRLTHVCQTKPFDSADFFENEFQIERLA
                     AQIEVYELILKKVIEENIDVQ"
     gene            complement(5331..7091)
                     /gene="efrB"
                     /locus_tag="SCJ86_001947"
     CDS             complement(5331..7091)
                     /gene="efrB"
                     /locus_tag="SCJ86_001947"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_029485765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux ABC transporter subunit EfrB"
                     /protein_id="HEG1169484.1"
                     /translation="MKKTFSSLKRLARYIRPYRETFLLVILFTVLTVAFNVALPYVTG
                     LPTTEISRNIANGESINFSYIFKCLIWITVVGIGYCISQLLSGVLMTNVVQSAMHDLR
                     RDIEEKINRLPVSYFDKNQQGNILSRVTNDVDAVSGALQQAFIGIVNAILGITLAAAM
                     MFYIQPMMALISMIMIPTSIWISKRVINASQKYFQSMQNSLGELNGYVQENMTGFSVL
                     KVYGREKETLEGFKKVNHSLKYFGFRAAFISGLMMPLVQLTAYATYIGMAVLGSFYVI
                     TGAIVVGQLQAFIQYIWQISQPMGNVTQLSSVLQSASAATKRVFEILDEPEEKENKVD
                     ASLPETILGDVTFDHVDFAYDPKKPLIQDLNFEVKAGQTVAVVGPTGAGKTTLINLLM
                     RFYDVDKGAIRIDGIDTKSMSRSDVRSLFGMVLQDAWLYQGTIADNIRFGKLDATDYE
                     VVDAAKTANVDHFIRTMPDGYDMAINSEGDNVSLGQKQLLTIARAVVSDPKILILDEA
                     TSSVDTRLEALIQKAMDRVMEGRTSFVIAHRLSTIRDADLILVMDQGQIIEKGTHESL
                     LAQGGFYEKLYNSQFAEEAE"
     gene            complement(7088..8818)
                     /gene="efrA"
                     /locus_tag="SCJ86_001948"
     CDS             complement(7088..8818)
                     /gene="efrA"
                     /locus_tag="SCJ86_001948"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux ABC transporter subunit EfrA"
                     /protein_id="HEG1169485.1"
                     /translation="MKLMWHYTMRYKKFLFFNFICVFGFILIELGLPTILARMIDVGI
                     RNDDFDYVKQQGIFMIVITVIGIILNILLGYFGSRITTNIVADIRDDLFEKIQSYSHQ
                     EYENLGVSSLITRTTNDAYQIMLFLQNILRIGFMAPMMFIVSLYMVMRTSVTLSLYVV
                     GALPLLLLAVVAIAKVSEPLSKKQQKNLDKINSILRENLSGLRVIRAFVNEKFEEKRF
                     AVVNENYASSSKSLFRLMAVAQPGFYFLFNIVMVLIIWSGAVQIDAGELRVGDLIAFI
                     EYIFHALFSFMLFASVFMMYPRAAVSAGRIQEALDAKPSIVEDPNGISDTKTKGYIEF
                     QNVTFAYPGHSQEPVIRNVSFTASPGETVAFIGSTGSGKSTLIQLIPRFYDVSEGKIL
                     IDGVDVRDYRLSKLRDKIGFIPQKALLFTGTIADNLRYGKEDATQEEMERAAEIAQAS
                     EFISRKPDGFNEHLSEGGSNFSGGQKQRLAIARAIIRRPEIYIFDDSFSALDYQTDAK
                     LRARLKKETTESTVLIVAQRVGTIMHADKIIVLNEGEVVGMGTHRELLEKCPIYYDIA
                     ASQLSKEELA"
     gene            9211..9423
                     /locus_tag="SCJ86_001949"
     CDS             9211..9423
                     /locus_tag="SCJ86_001949"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-dependent RNA polymerase auxiliary subunit
                     epsilon family protein"
                     /protein_id="HEG1169486.1"
                     /translation="MIYKVYYQETKVRNPKREETKSLYIEAKSDVDARQRVEENTPYN
                     IEFVQLLEGSHLEYEKENADFSLVEF"
     gene            9425..11104
                     /locus_tag="SCJ86_001950"
     CDS             9425..11104
                     /locus_tag="SCJ86_001950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease J"
                     /protein_id="HEG1169487.1"
                     /translation="MKLSIKNNETGVFAIGGLGEIGKNTYGVQFQDEIILIDAGIKFP
                     EDDLLGIDYVIPDYSYIIQNLHKVKALVITHGHEDHIGGIPYLLKQANIPIYAGPLAL
                     ALITNKLDEHGLLRDAELHEINEDTIIRFRKTSVSFFRTTHSIPDALGIVVKTPSGNI
                     VATGDFKFDFTPVGEPANLHKMARIGEEGVLCLLSDSTNAEIPTFTKSEKTIGHSILK
                     IFSKIEGRIIFASFASNIFRLQQAADAAVATGRKIAVFGRSMENAIVNGQRLGYIKVP
                     DGTFVDAHEINRLPANETMILCTGSQGEAMAALSRIANGTHRQIQIQPDDTVIFSSSP
                     IPGNTTSVNHLINLLSEAGAEVIHGKINNIHTSGHGGQEEQKLMLRLMKPKYFIPVHG
                     EFRMLKVHASLAQDVGVPAENCFIMENGDVLALTSDSARPAGHFVAGDVYIDGSGIGD
                     IGNVVLRDRHLLSEEGLVLAVATVDIKKKEILAGPDILSRGFVYMRESGEMINEAQRI
                     LFHALRECLNGPDCSEYKLKEAMTGALQPFLFERTERHPMILPMVMTPDEE"
     gene            complement(11358..11558)
                     /locus_tag="SCJ86_001951"
     CDS             complement(11358..11558)
                     /locus_tag="SCJ86_001951"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003234247.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cold-shock protein"
                     /protein_id="HEG1169488.1"
                     /translation="MENGTVKWFNAEKGFGFISREDGSDVFVHFSAIQGDGFKTLEEG
                     QAVTFDVEESDRGPQAANVVKA"
     gene            complement(11708..11851)
                     /locus_tag="SCJ86_001952"
                     /pseudo
     CDS             complement(11708..11851)
                     /locus_tag="SCJ86_001952"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326507.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein"
     gene            complement(12086..12748)
                     /locus_tag="SCJ86_001953"
     CDS             complement(12086..12748)
                     /locus_tag="SCJ86_001953"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016922249.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HEG1169489.1"
                     /translation="MNTIESQFDKVAEDYDFVNELLNDYSFFVSNMSPKKGRALDIGC
                     GSGLLVEKLASYYDEVVGIDISNQMLDLAKSKRQLTNTVYLNMNAEQLNFNEKFDFIV
                     SRTTFHHLDDIASVIQQMKELLNEEGRIVILDNVSEVETPPTYVYKLGAIQEFLPHCF
                     KFGIKNAIRIYNHNTSKSWLEHLASDKYLSEQNYYDLYEKLLPGCQFHKMGWAMGVVW
                     TK"
     gene            13140..13199
                     /locus_tag="SCJ86_001954"
     CDS             13140..13199
                     /locus_tag="SCJ86_001954"
                     /inference="COORDINATES: protein motif:HMM:NF033690.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="erythromycin resistance leader peptide"
                     /protein_id="HEG1169490.1"
                     /translation="MGTFSIFVINKVRYQPNQN"
     gene            13257..13988
                     /gene="erm(A)"
                     /locus_tag="SCJ86_001955"
     CDS             13257..13988
                     /gene="erm(A)"
                     /locus_tag="SCJ86_001955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001072197.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (adenine(2058)-N(6))-methyltransferase
                     Erm(A)"
                     /protein_id="HEG1169491.1"
                     /translation="MNQKNPKDTQNFITSKKHVKEILNHTNISKQDNVIEIGSGKGHF
                     TKELVKMSRSVTAIEIDGGLCQVTKEAVNPSENIKVIQTDILKFSFPKHINYKIYGNI
                     PYNISTDIVKRITFESQAKYSYLIVEKGFAKRLQNLQRALGLLLMVEMDIKMLKKVPP
                     LYFHPKPSVDSVLIVLERHQPLISKKDYKKYRSFVYKWVNREYRVLFTKNQFRQALKH
                     ANVTNINKLSKEQFLSIFNSYKLFH"
     gene            complement(14114..14896)
                     /locus_tag="SCJ86_001956"
     CDS             complement(14114..14896)
                     /locus_tag="SCJ86_001956"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000067268.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminoglycoside nucleotidyltransferase ANT(9)-Ia"
                     /protein_id="HEG1169492.1"
                     /translation="MSNLINGKIPNQAIQTLKIVKDLFGSSIVGVYLFGSAVNGGLRI
                     NSDVDVLVVVNHSLPQLTRKKLTERLMTISGKIGNTDSVRPLEVTVINRSEVVPWQYP
                     PKREFIYGEWLRGEFENGQIQEPSYDPDLAIVLAQARKNSISLFGPDSSSILVSVPLT
                     DIRRAIKDSLPELIEGIKGDERNVILTLARMWQTVTTGEITSKDVAAEWAIPLLPKEH
                     VTLLDIARKGYRGECDDKWEGLYSKVKALVKYMKNSIETSLN"
     gene            complement(15050..15427)
                     /locus_tag="SCJ86_001957"
     CDS             complement(15050..15427)
                     /locus_tag="SCJ86_001957"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000361059.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HEG1169493.1"
                     /translation="MDKQVRNTTEIVRLAKQKSKKTREKVDKAISKFSIEGKVINFNS
                     IAKEANVSKSWLYKEHDIRQRIESLRERQITANVVSKPKKSSRSEEILIKTLKRRVME
                     LEKENKKLQNQIQKLYGDLYNKE"
     gene            complement(15434..17326)
                     /locus_tag="SCJ86_001958"
     CDS             complement(15434..17326)
                     /locus_tag="SCJ86_001958"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001833801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HEG1169494.1"
                     /translation="MNASSKRKIISQSEISKKIAVMNEEMQGFWANNSWDIRKCPHPS
                     AIELSKNPALRNRWVRFERVKNLWLRTELKYFYFYHLNNGIWNAKTVWIRKGTVINKM
                     LDFLDLKYPSITSITEVPIEKAMTEYRTYLTKRGVRITTTNYKITANQEKTPVKANSY
                     YVTNLKQFMEFYENFYFDGEEWDKDVWDRRNLPLPDDKVNPTQYEYTINFKGFRNTYF
                     KQLVKRYCKLRLNMDSFSYVSDIAQRLKEFFNFLDMKFKQVQRVHQLTRVEIEAYLSE
                     LNMMGIKPSTITGRISILEGLFSTLLRLEWDDVPSKILIYSEDYPKIPRAKPRFIDEF
                     VLEQLNSHLDKLPEYIATMTMIVQECGMRISELCTLKKGCLLEDKDGDFFLKYYQWKM
                     KKEHIVPISKEVALLIKVREDKVSEEFPDSEYLFPRKDGSPLKQETFRGELNKLAYEQ
                     NIVDKSGEIYRFHAHAFRHTVGTRMINNGMPQHIVQKFLGHESPEMTSRYAHIFDETL
                     KNEFTKFQEKLVTNNGDVLDLDEDNEVDDVELQWFKKNINAQVLPNGYCRLPVVAGGC
                     PHANACLDCTHFCTSKQFLPQHEEQLERTEELLAIAKDKQWQRQVETNSRVKERLEQI
                     IGSLTG"
     gene            complement(17323..17883)
                     /locus_tag="SCJ86_001959"
     CDS             complement(17323..17883)
                     /locus_tag="SCJ86_001959"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002360833.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HEG1169495.1"
                     /translation="MIDACHTKRDKLILMLMYEGGLRIGEVLSLRLEDIVTWDNQIHL
                     TPRDVNVNEAYIKLRKERTIHVSKELMSLYTDYLIYEYSEELEHDYVFISLKEGYFGK
                     PLKYQSVLDLVRRIVKRTGIEFTSHMLRHTHATQLIREGWDVAFVQKRLGHAHVQTTL
                     NTYVHLSDQDMKNEFNKYLERKEHKK"
BASE COUNT         5446 a         3791 c         2946 g         6096 t
ORIGIN      
        1 ttaaaaaaat aaaattttat tggttttttc ttaaccattt tgaattggtt tttgcaaacc
       61 ctttaacttc atattcgttt gtttcttcca tgtacgcttc gtccaaaacc attgtttgtc
      121 ttctcaactc gcttaatttc tgtccttcgt tgcttggcac agaaagtgta taaggggtca
      181 ttagttccat cagctgacgt ttgattgctt cgatcaacgc catcttacct tctttcgtct
      241 gagtagaaat caatacattt gggaatagcg taggcgtgaa catcgctggg tcgatctggt
      301 ctgccttatt gtaaacagtc agtatcggca tttctttcat attcagttca tccatcaatt
      361 ttaagacagt ctgctcctgt aaaatacgat ctggagaact agcatcgact acatgtagca
      421 aaagatccat attttgactt tcttctaatg tagaatggaa agcatcgatc agttgcgtag
      481 gcaggtcttg gatgaacccg acggtatccg ttacagtaat ctcaaatcct tcagcaaaac
      541 gccatctctt agtcaatgga tctaacgttg caaacaattg atctttcgaa taagtatccg
      601 cttgtgtcaa tagattcaag atagttgact tcccggcatt cgtgtagcca ataagtccaa
      661 tctgaaaaat ctcgctattt tttctttttt ggcgattgcg ctctcgatga gcttctactt
      721 ctttcaattc tttcttaaca gcaaaaattt tattccgtat atgtcggcgg tctgtttcta
      781 actttgtttc acctggccct cttgttccga tcccaccgcc aagacgagat aatgttttcc
      841 cttgccctac taaacgaggc agcaggtatt ctaactgagc aagttcaacc tgcagcttcc
      901 cttctttcga acgagcacgc atcgcaaaaa tatctaagat aagctgcacg cgatcaatta
      961 ccggaagacc aacagcatcg gtaatcagtt ggctctgtct cggagttagc tcatgattaa
     1021 agatgatcaa atccgcttca tgcgcatccg cttgttgaac taattcttgt aatttccctt
     1081 taccgataat cgtttgccga tccacttgtg gtctcttttg tgtaattgtg aatacgactt
     1141 ctcctgatgc ggtattcgtc agctgaacta gctcttccat tgactctgaa aagtaacgct
     1201 ggtttgcttc ggtttctacc cctacgatga tcactttttc ttccatttta ttccgcctcc
     1261 agccattttt tgattgtttg ctcaatctga cttttttgat ttggctcact tataagatca
     1321 aaccactgtg catccaaacg gttacgaaac cacgtcaact gacgtttcgc gtatcttctc
     1381 gaatgtagtt tgacttgttc tgttgcttct tccaatgttt ctttcccttc aaaataaggg
     1441 aaaaattctt tatatccaat accttgactg gcctggactt ctccaagctc atacacctta
     1501 cgcgcttctg ctactaaacc ttctgctagc atcaaatcga ctctttgatt gattcgttca
     1561 taaagaatac tacgcttcgt atctaatcca atcatgaggt aatcatacaa ttgctttggc
     1621 tcttctttag gtgccgtgat actatgtcct gtcgtctcta aaacttccag agctcttatc
     1681 actttgcgtt gattgttcca gtggattttc tcagcagctg ccgggtcttt ttctttcaac
     1741 tgattccata atgcttggga accaattgtt tcagctaacg tttcatactt ttttctaatc
     1801 tcaggattcg cttcttctgt cgctcctaac tgataatcat atagaaggga ttggatatag
     1861 agacccgtac caccagctat gataggtagg tgccctcggt tcgcaatctc ggtaatcaat
     1921 tgtcgacctg aatgctgaaa atcagcggct gagtaacgtt gagtaggttc gatcacatca
     1981 atcaaatgat gcggaacatt attcatctct tcttctttga tttttgctgt accgatatcc
     2041 agatttttat agatttgcaa tgaatcacca ctgatgatct ctcctccaaa tagttgtgcg
     2101 agttccacac ttagcgctgt tttaccgact gctgtcggac cgacgatcac taatactttt
     2161 tccatatttt acctactttt cgttgattgt ttccaccgaa gagctttctc cggaaaatca
     2221 gttattattc catctatttt gagttcttga catttggcca tttcttcttc atcgttaatc
     2281 gtccatgcac gaattgcttt tggaaaatca gctgcctgga ctggatgttc gaacaaccaa
     2341 gtattttttg gatgtactcc ttctatataa ggggtttctg ctgctagttg gatcttttta
     2401 tcagaagtac tcatcaataa gtccagctgt atctccggtt ccagtaatgc gattttttcc
     2461 aaagaatcca aatgaaagct gcaatatgca tgttgaaaag gccaatctct actttttaat
     2521 gtttcggcta atttttcttc gataccggga tattcatact gatcagtttt aatttcaatc
     2581 attagcatcc ctcggtaatt tttttggagg agcagtccta acacttctct caattcagga
     2641 actttagctg ctggaaattc tttgctgaat ttgccccctg cattcaattt tttgagttct
     2701 tccaatgttt tttctcgaat cagcccttta ccgttagtcg tccggtccac ttcttcgtcg
     2761 tgcataacga tcaaataacc atctttgctt agatggacat ctagttcgat accatctgca
     2821 cctactcgta cagcttctgc aaatgcagga agcgtatttt ctgggtgggt gccgctgctt
     2881 ccgcgatggg catagatttg taacatcttt cttcactccg ttcagttcta ttgtatcatt
     2941 aagaaaaaaa gaaagcatta ccacgctctc atgtgcaacg gcaatgcttc aaaatatcag
     3001 ctccaaacat catctttcga gaagaaacca atcggctggc gcgtcatagt cttcaactga
     3061 gctttttttt cattgatcat ttcagtcaga ctatccgtaa aattctctaa aatcacgcgt
     3121 ccgcctttat aagcatatcc accaatcttc attgtttcat ctggagataa aatcactttt
     3181 cgatgggaga tattcttaaa tagctcgtct aaaatataat ttggcagata aaaactattt
     3241 tgcgctgaaa atgattggag atacgcaaaa tcgtcaatcg tgatataaca atcatcaaat
     3301 gcatcaaagc tgacatattc ttccatcagc gttgtccgtt tttgcaagat ctcgttgatc
     3361 tttttattta attgattcag cgtctcgatt tctttgactt tcaattccaa ttcacgtcta
     3421 cgctgttctt tattttccaa gtgacgcatt tgcagtatca tgccgatata gacaactaca
     3481 gcacctgtca gcactccgat aaggccagta ataccaacta tcaatctgtt gttcctcctt
     3541 gttttgtttc tctgcttatc ataccataaa gcgggtttat gcgactaatt tgcctaagtt
     3601 ttttatttga ccttttttga ccaaaaatgt atactacatt ttaggtatta gaaactaaat
     3661 tatggtacac tatttctaaa cgaaatcaag gaggaactct attatgaatt taattcctac
     3721 agtcattgaa caatcttcac gaggtgaaag agcttacgat atctactcac gtttattgaa
     3781 agaccgtatc atcatgttaa gcggccaagt aacagatgat cttgctaatt cgatcatcgc
     3841 ccaattgctt ttcttagacg ctcaagattc tgaaaaagat atctatcttt atatcaattc
     3901 tcctggtggt tcagttacag caggtatggc tatttacgac acaatgaact ttgtcaaagc
     3961 agatgtccaa acaattgtta tgggtatggc tgcttcaatg ggaagcttct tgctgacagc
     4021 tggtacaaaa ggtaaacgat ttgctttacc aaatgctgag atcatgatcc atcaacctct
     4081 tggcggcgct caagggcaag caactgaaat cgaaattgcg gcacgccata ttttgcaaac
     4141 acgcgaacgc ttaaacaaaa ttttagctga acgcactgga caaccgcttg aagtgattga
     4201 aaaagataca gatcgtgaca actatatgac agctgaacaa gcaaaagcat atggattgat
     4261 cgatgaagtc atggaaaaca gttcttcatt aaattaatct gtttcaaagg aagctttttt
     4321 aagctctctt atctaaacca aataaaaaag gatggtcaac tcaaactgac catccttttt
     4381 tattttatgg atgctactgc ctatctttgc tccttgaatt ttccttgcgc atgcatccga
     4441 cctatctcga cataatgctg gatgttttct ttgttttttt gtatttcttc ttttgtcaaa
     4501 ggacggatca ctttagctgg gcgaccaaaa gccaagacat taggaggaat aacttttcct
     4561 tctgttacta atgatccagc accaatcaaa ctattctcac caataaccgc atgattcaat
     4621 atcgtggtcc ccatcccgat cagcgcccct ttttcgattg tacagccatg tagcatgcac
     4681 tgatgtccta ccgtcacatt ctccgcaata tctacaggtg catcatgatc gacatggatg
     4741 attgtaccat cttggatatt cgttctttgt cctattttta tccaattact gtccccgcgt
     4801 aaaacagctt gataccagat cgtcacatct tctgacaatg tcacatcacc aatcactgtg
     4861 gcatttgatg ctataaaccg ttccatattt atttttctcc tttcattttt tgtagaaact
     4921 aatcctattg aataaaggta tcatagcata tatttatttc ataagaaaaa aaacaggtaa
     4981 aacctgctca ggccagatgt ctatttacag tatctggtaa ctgacgtttt acctgttttt
     5041 attgtacatc tatattttct tctattactt tttttaaaat taattcgtac acttctattt
     5101 gtgctgccaa gcgttcgatt tgaaactcat tttcaaaaaa atcagctgaa tcaaaaggct
     5161 tcgtctgaca gacgtgcgtt aaacgatcaa cttctctttt ggcttcagta attttttcat
     5221 ttaaaaactg ttccatctcc gtgtcctcac ttttcttgat aactttactt taccattatc
     5281 aagaatagag aacaaaaaat aagttttaaa ctgttttatt cttatatgac ttattctgct
     5341 tcctcagcaa attggctgtt atacagtttt tcataaaatc caccttgtgc caataaactc
     5401 tcatgcgttc ctttttcaat gatctgacct tgatccatca ctagaatcag atctgcatca
     5461 cggattgtcg ataaacggtg agcgatgacg aaacttgtcc gtccctccat cacgcgatcc
     5521 attgcttttt gaatcaacgc ttctaaacgg gtgtctactg aacttgttgc ttcatctaaa
     5581 atcaagattt taggatcgga gaccactgct cgggcaatcg tcagcaactg tttttgtcct
     5641 aaagacacat tgtctccctc agaattgatg gccatatcat atccatctgg catcgttcgg
     5701 ataaaatgat ctacgtttgc tgttttcgca gcatcaacta cttcataatc tgtcgcatca
     5761 agctttccga agcggatatt atctgcaatc gttccttgat aaagccatgc atcttgcaag
     5821 accataccaa atagcgaacg cacatcactg cgactcattg atttggtgtc gattccatca
     5881 atccttattg ctcctttgtc tacgtcataa aaacgcatca atagattgat caatgtcgtc
     5941 ttgccggcac cggtcggacc gaccacagct acggtttgac cagctttcac ttcaaaattc
     6001 aaatcttgga ttaatggttt cttcggatca tatgcaaaat cgacatgatc gaatgtcaca
     6061 tctcctaaaa tcgtttctgg cagtgaagca tctactttgt tttctttttc ttctggctca
     6121 tctaaaatct cgaatacacg tttcgttgcc gctgatgcac tttgcaaaac tgaagacaat
     6181 tgtgtcacgt tccccattgg ctgactgatt tgccaaatat attggatgaa tgcttgtagt
     6241 tgaccgacaa cgatcgcccc agtaataaca taaaagcttc ctagaacagc cataccaata
     6301 taagtagcat aggcagttaa ttgaactaga ggcatcatca agccagaaat gaatgctgca
     6361 cgaaacccaa agtatttcaa gctgtgatta acttttttga atccttccaa tgtttctttt
     6421 tcgcgtccat atactttcaa tacactaaaa cctgtcatat tttcttggac gtagccgttt
     6481 agctcaccaa gagaattctg catgctttga aaatatttct gcgaagcatt gatcactcgt
     6541 tttgagatcc agatcgatgt tggaatcatg atcatagaga tcagcgccat catcggctgg
     6601 atataaaaca tcattgctgc agccaacgta attcccaata tcgcatttac gataccaata
     6661 aaagcttgtt gtaaagcacc gctgactgca tctacatcat tcgtcacacg agacaaaata
     6721 tttccttgct ggtttttatc gaaatatgaa accggaagac gattgatttt ttcttctatg
     6781 tctcttctca aatcatgcat ggcactttga acgacatttg tcatcaaaac accagaaagt
     6841 aattgcgaaa tacaataccc tattccaaca actgtgatcc aaatgagaca tttgaatata
     6901 taagaaaaat tgattgattc gccattggca atattacggc tgatttctgt agtaggaagt
     6961 cctgtaacat atggcaaagc cacgttgaaa gccactgtca gaactgtaaa taggatcact
     7021 aaaagaaaag tctcacgata cggtctgata tatcgagcta aacgttttaa ggaagaaaag
     7081 gtttttttca tgctaattcc tcctttgata attgagaagc ggcaatatca taataaatcg
     7141 ggcatttttc caacaactcc cgatgcgtcc ccataccgac gacttctcct tcatttaata
     7201 cgatgatctt atctgcatgc atgatcgttc ccacacgttg tgctacgatc aatacagtag
     7261 attctgtcgt ttcttttttc agacgagcac gtaacttcgc atccgtttga taatctaatg
     7321 cagaaaagct gtcatcaaaa atatagattt ccggacggcg tatgatggct ctggctattg
     7381 ccaatcgttg tttttgtcca cctgaaaaat tggaaccacc ttctgataaa tgttcattaa
     7441 atccatctgg ttttcgagaa atgaattctg aagcttgagc aatctcagct gcacgttcca
     7501 tttcttcttg cgtagcatct tctttcccgt atcgcaaatt atctgcaatc gttccagtaa
     7561 atagcagcgc tttttgcgga ataaacccaa tcttatctcg caacttgctt agccgataat
     7621 ctctcacgtc tactccatcg atcaagatct ttccttcact cacatcataa aagcgaggga
     7681 tcaattggat caacgtagat tttccgctac cggtacttcc aataaaagca acggtctctc
     7741 ctggagaggc tgtaaaactg acatttcgta tcactggctc ttgcgaatgg cctggataag
     7801 caaatgttac attttgaaat tcgatatacc ctttagtttt tgtatcagat ataccatttg
     7861 gatcttctac aatcgaaggt ttggcatcta gggcttcttg gatacgtcca gctgaaacag
     7921 ctgctcttgg atacatcatg aacacactag caaataacat gaatgagaac aaggcatgga
     7981 atatatactc gataaacgca atcaaatctc ctacacgaag ctcgcctgca tcgatttgca
     8041 ctgccccgct ccagatgatc aataccatta cgatattaaa taagaaataa aaacccggtt
     8101 gtgcaacagc catcaaacgg aataagcttt ttgaactaga agcataattc tcattgacta
     8161 cagcaaaacg tttttcttca aacttttcat tgacgaatgc acgaatcaca cgcaatcctg
     8221 aaagattttc ccgcaagata ctgttgattt tatctaagtt tttttgttgc tttttagata
     8281 acggttcaga caccttagca atcgctacga ctgccaataa tagtaaaggt aatgcaccta
     8341 ctacatacaa actgagtgtc acacttgtac gcatgaccat gtacaaactg acaatgaaca
     8401 tcattggtgc cataaaaccg atacgtaaaa tattttgcag gaacaacatg atctgataag
     8461 catcgtttgt tgtacgagtg atcaatgaag atacgcctag attttcgtat tcctgatgcg
     8521 aataactttg gatcttttcg aacaaatcat ctcgaatatc tgctacgata ttagtcgtaa
     8581 tcctcgaacc aaaataacct aacaagatat tcaaaataat accgattaca gtaataacga
     8641 tcataaatat cccttgttgc ttcacataat caaaatcatc attgcggatt cctacatcga
     8701 tcatacgtgc taaaatcgta ggaagtccta attcaatcaa aataaaacca aatacgcata
     8761 tgaaattgaa aaacaagaat ttcttataac gcatggtata atgccacatt agtttcactt
     8821 tctcatctcc ttttaatctt ttgttacatt ttcattacta aaaatgatag cacaacgagc
     8881 ctattaatag aacacttctc tcataaaaaa gtcattatga ttatagcggg atgataaaca
     8941 ttgatttttt ctttcaatct agttgatttc tatacttatt tgctgtttta tgctagaata
     9001 acaattaatt gttgtatatt ttttatacag aaggtacggt tgaaaaaata tccgaacaga
     9061 taagcaaaaa cgaggcttgt ctctttgact cgaatccagt attctagtca aagcatatga
     9121 aaaaccaggt ggcttgtccc ctcgttttgt gttgtctaaa aaaatcaact agctaaatca
     9181 ctgctttacg cagataaagg agacaaaaca atgatctata aagtttatta tcaagaaaca
     9241 aaagtacgca accctaaacg ggaagaaaca aaatctcttt acattgaggc aaaaagcgat
     9301 gtagacgctc gccaacgtgt ggaagaaaat accccataca atattgaatt cgttcaactt
     9361 ctagaaggaa gtcatctaga gtacgaaaaa gaaaatgctg atttctcatt agtggagttt
     9421 taatatgaaa ctttccatca aaaataacga gacaggcgtc tttgctattg gtggattagg
     9481 tgaaatcggg aaaaatacgt atggggtaca attccaagat gaaatcattt tgattgatgc
     9541 cgggatcaaa ttcccggaag acgatttact tggaattgac tatgtcattc ccgactacag
     9601 ctacatcata caaaacttac acaaagtaaa agccttggtg attactcacg ggcacgaaga
     9661 ccacatcggc ggaataccct acttgctgaa acaagcaaat attcctatct atgctggtcc
     9721 tctcgcatta gcgttgatca caaacaaatt agatgagcat ggcttgcttc gagatgcaga
     9781 gctccatgag atcaatgaag atacgatcat ccgttttaga aagacttctg tcagcttttt
     9841 cagaacgacc catagtatcc cagatgcatt agggatcgtg gtgaaaacac cttctggaaa
     9901 tatcgtagcg actggagact tcaaattcga tttcactcct gttggcgaac cagcaaactt
     9961 gcataaaatg gctcgtatcg gggaagaagg agtcttgtgc cttttatctg atagtacaaa
    10021 tgctgagatt ccaacattta ctaaatcaga aaaaacgatt gggcattcta ttttgaagat
    10081 cttttcaaaa atcgaaggac gtatcatttt tgccagcttt gcttctaaca tcttccgttt
    10141 gcagcaagca gcagatgccg ctgtggcaac tggtcgtaaa atcgctgttt tcggacgaag
    10201 catggaaaat gccatcgtca acggtcaacg tttaggatat atcaaagttc cagacggcac
    10261 atttgtagat gcacacgaaa tcaatcgctt gcctgccaat gaaacgatga tcctatgtac
    10321 aggttctcaa ggagaagcga tggccgcgtt gagccgcatc gcgaatggta ctcaccgtca
    10381 aatccaaatc cagccagacg atacagtgat cttttcaagt tcacctattc ctggtaatac
    10441 aacaagtgtc aatcatttga tcaatttgct gtctgaagct ggtgcagaag tgattcacgg
    10501 aaaaatcaat aacatccata cttccggtca cggtggacaa gaagaacaaa aattaatgct
    10561 tcgtttgatg aagccaaaat attttattcc tgtccacgga gaattccgca tgttgaaagt
    10621 acatgcttct ctagcacaag atgtaggtgt tcctgcagaa aactgcttca tcatggaaaa
    10681 tggcgatgtt ttagcgttaa ctagcgattc tgcgcgtcct gctgggcatt ttgtggctgg
    10741 agatgtgtat atcgatggta gcgggatcgg tgatattggt aatgtcgttt tacgtgaccg
    10801 tcacctgctt tcagaagaag gtcttgtatt agctgtcgcg acagtggata tcaagaaaaa
    10861 agaaatcttg gctggtccag atattctctc tcgtggattt gtctacatgc gagaatcagg
    10921 agaaatgatc aatgaagctc agcgaatctt attccacgct ttaagagaat gtttgaatgg
    10981 acctgactgt tcagaatata aattgaaaga agcgatgact ggcgcgcttc agcctttctt
    11041 attcgaacgt acagaaagac atccaatgat tttgccaatg gtaatgacac cagatgaaga
    11101 ataaataaga ggttatgaaa tcagcacctt gtcctgaata taagagaaaa caaatgaaaa
    11161 acatgagaag ccatttttgt ttgttcttat tccttgatac aaggtggctc tttcatagcc
    11221 tcttaaattt atttactttt tcatacaaaa aaatacctgc cacaagagaa attgtctctt
    11281 gtggcaggta tttttgttac tcgctttgtt ctctcgtaaa aattgatgtc taccatcaat
    11341 ttgatttaca gataaaatta agcttttaca acgtttgcag cttgtgggcc gcggtctgat
    11401 tcttcaacgt cgaaagtcac tgcttgacct tcttctaatg ttttgaatcc gtcaccttgg
    11461 atagctgaga agtgaacgaa tacgtcgctt ccatcttcac gtgagataaa tccaaatcct
    11521 ttttcagcgt tgaaccattt tactgttccg ttttccataa taatatcctc ctcgtgccaa
    11581 aagcactaat ttaaattgta acattgctcg tgtacgatag aggtgcaaca gatgtttcaa
    11641 cttctattcc caaacaaaat tacaatataa ttgtacactt gcttcatttt ttttgcaaga
    11701 aagcaactta cttctcttcc atcattcctt cttctctcaa actaaaatat cgtttgcgtc
    11761 cgatgatcaa gtggtccagt acagtgatcc ccatcatttc tccacacttt tgtatccttt
    11821 tggtaaaatt catatcctgc tgcgatggaa tttcatatta tcaagagtaa attacaaaag
    11881 aatcatttaa atcaatactt tcactttgaa tacatgtatt tgaagtggaa ggtacttatt
    11941 tcaaaaatag taaaacctgt atcttaaatt acttaatagt aacataagat acaggctgat
    12001 tttttattca ttgttattta tactaaagca cccgatagct ctgaaaacaa tcacaaattc
    12061 aacttttcaa agccacagct ttaagttatt ttgtccagac aacccccatt gcccaaccca
    12121 ttttatggaa ttggcatcca ggcaacaact tttcatataa atcgtaataa ttttgttcag
    12181 ataggtactt atctgaagct aaatgctcaa gccatgattt agatgtgttg tgattataaa
    12241 ttctaatcgc attttttatt ccaaatttga aacaatgcgg taaaaactct tggattgccc
    12301 ctaatttata aacataggta ggcggtgtct cgacttcaga tacattatca agaataacta
    12361 ttcttccttc ctcattcaac agttccttca tttgctgtat tacgctggct atatcatcca
    12421 aatgatgaaa ggttgttcgg cttacaataa aatcaaactt ctcattaaaa ttaagttgtt
    12481 ctgcattcat attcagatag accgtatttg ttagttgacg tttagatttg gcaagatcga
    12541 gcatttgatt agaaatatca atccctacca cttcatcata ataacttgct aatttctcca
    12601 ctaacaaacc cgagccacat ccgatatcta atgctctgcc tttctttgga gacatattag
    12661 acacaaagaa tgaataatca ttcaaaagct cattcacgaa atcgtaatct tctgcaacct
    12721 tatcaaactg tgattctatt gtattcaaaa agatccccca ttcctacttt atcgacattc
    12781 tttcattact taccacttta gatgtttttt cgttggggat aaaacttccc tttagacaat
    12841 tttatccaaa gacaatacaa cagtgcaact ttattaaagt cactgtcctt tatcgcagcc
    12901 tttacttttt agtaaagaca gtggcttctc ttatcaagtt tcaaaacata ttattttgaa
    12961 gaaaacgtcc atctgaagtg tcaagtgcaa aattacatat aaaggtttat tctaaaatga
    13021 aaagatgata caatcatatt cagttacata aggaggtttc aattatgtgc accagtatcg
    13081 cagtagtaga aattacttta tctcattcat aatgaaaaaa atggaaagga gataaaagta
    13141 tgggtacttt ttctatattt gttattaata aagttcgtta tcaaccaaat caaaattaat
    13201 tggttataat gaacgcttaa tgtcagttca ttataaccag taaggagaag gttataatga
    13261 accagaaaaa ccctaaagac acgcaaaatt ttattacttc taaaaagcat gtaaaagaaa
    13321 tattgaatca cacgaatatc agtaaacaag acaacgtaat agaaatcgga tcaggaaaag
    13381 gacattttac caaagagcta gtcaaaatga gtcgatcagt tactgctata gaaattgatg
    13441 gaggcttatg tcaagtgact aaagaagcgg taaacccctc tgagaatata aaagtgattc
    13501 aaacggatat tctaaaattt tccttcccaa aacatataaa ctataagata tatggtaata
    13561 ttccttataa catcagtacg gatattgtca aaagaattac ctttgaaagt caggctaaat
    13621 atagctatct tatcgttgag aagggatttg cgaaaagatt gcaaaatctg caacgagctt
    13681 tgggtttact attaatggtg gagatggata taaaaatgct caaaaaagta ccaccactat
    13741 attttcatcc taagccaagt gtagactctg tattgattgt tcttgaacga catcaaccat
    13801 tgatttcaaa gaaggactac aaaaagtatc gatcttttgt ttataagtgg gtaaaccgtg
    13861 aatatcgtgt tcttttcact aaaaaccaat tccgacaggc tttgaagcat gcaaatgtca
    13921 ctaatattaa taaactatcg aaggaacaat ttctttctat tttcaatagt tacaaattgt
    13981 ttcactaaat taaagtaata aagcgttctc taatttcaca agaggacgct ttattcttcc
    14041 caaaaattgt tcaatattta tcaataaatc agtagtttta aaagtaagca cctgttattg
    14101 caataaaatt agcctaattg agagaagttt ctatagaatt tttcatatac ttaacgagtg
    14161 ctttcacctt tgaatatagt ccttcccact tatcatcaca ctctccccga tagccttttc
    14221 tagctatatc cagtaaagtt acatgctctt taggtaaaag aggtatagcc cattctgcag
    14281 cgacatcttt cgaggtaatt tcaccagtag tcactgtttg ccacattcga gctagggtta
    14341 aaattacatt acgctcatca ccttttatcc cctcaattag ttctggcaaa gaatccttaa
    14401 ttgctcttcg aatatctgtc aaaggtacgg agacaagtat acttgaagaa tcaggaccaa
    14461 atagagaaat actattcttt cttgcttgtg ctaaaacaat agccaaatca ggatcatagc
    14521 ttggttcctg aatttgtcca ttctcaaatt cacccctgag ccactcaccg tatataaatt
    14581 ctctttttgg aggatattgc caagggacaa cttcactcct atttataacc gtaacttcaa
    14641 gtggtctaac agaatccgta tttccaatct ttcctgatat agtcattagt ctttctgtta
    14701 gtttttttcg agttaattga ggtaaactat gattcacgac gactagaaca tctacatcgc
    14761 tgttaatgcg taaaccacca tttactgctg aaccaaatag atatactcca actattgaac
    14821 ttccaaataa atcttttacg atttttaatg tttgaatcgc ttgatttggt atttttccgt
    14881 taatcaaatt gctcatgatt tcacctcgta actgattatg ttcatataaa gtttatattg
    14941 atactcaatt tacttaccct agattggaca tatacctaaa tgactgttca ataaagctga
    15001 ccgttagcgt ttaagtacat cctttcacaa tttgtctaca gattaataat tattctttat
    15061 tatacagatc tccatataat ttttgaattt ggttctgtaa ttttttattt tctttttcta
    15121 attccattac tcttcttttt aaggttttaa taaggatttc ctccgaacga gaacttttct
    15181 tgggttttga gactacattt gctgttattt gacgctcacg aagggattcg attctttgcc
    15241 taatatcgtg ttccttataa agccatgatt tagaaacatt agcttccttt gctattgaat
    15301 taaaattaat aactttacct tcaatcgaaa atttagaaat cgctttgtct actttttccc
    15361 ttgtcttttt tgatttctgc ttcgccaaac gtacaatttc tgttgtattt ctaacttgtt
    15421 tatccattga taattacccc gtcaaacttc caatgatttg ttctaaacgc tctttaacac
    15481 ggctattagt ctctacttgt ctttgccatt gtttatcctt agctatggct aataactctt
    15541 ctgtacgctc taactgttct tcgtgctgtg gtaagaattg cttactggta cagaagtgag
    15601 tgcaatctaa gcatgcattc gcatgtggac aaccacctgc tactactggc aatctacaat
    15661 aaccatttgg aagcacttgt gcatttatat ttttcttgaa ccattgaagc tctacatcat
    15721 cgacttcatt atcttcatct agatcaagca catctccatt attggtaacc agtttttcct
    15781 gaaatttagt aaattcattt tttagagttt catcaaagat atgagcgtat ctgcttgtca
    15841 tttctgggct ttcatgcccc aaaaatttct gcacaatatg ctggggcatc ccgttgttaa
    15901 tcattcttgt tcctactgta tggcgaaagg catgggcatg gaatctataa atctcacctg
    15961 atttatccac tatattttgc tcataagcta atttatttaa ctcacctcta aatgtttctt
    16021 gttttaatgg cgatccatct tttcttggaa agaggtattc actatctgga aattcctctg
    16081 aaactttatc ttcccgaact ttaataagta aagctacctc tttagatatt ggaactatat
    16141 gctccttttt cattttccat tgataatact ttaaaaagaa atctccatct ttgtcctcta
    16201 atagacagcc ttttttcaag gtgcacaatt cacttatcct cattccacat tcttgaacaa
    16261 tcatagtcat cgtagctata tattcgggta atttatcaag atgactgttc aattgctcta
    16321 ggacgaattc atctataaag cgtggttttg ctcttggtat tttcggatag tcctcagaat
    16381 aaattaatat tttggaagga acatcatccc attctagcct aagaagggta ctaaatagtc
    16441 cttccaatat agagatcctc ccagttattg tactaggttt tattcccatc atgtttagtt
    16501 cacttaaata tgcttcaatt tccactctcg ttaattggtg tactctctga acttgtttaa
    16561 atttcatgtc cagaaaatta aagaactctt taagtctttg ggcaatatca cttacatagg
    16621 aaaagctatc catgttcaat ctcaacttac aatatctttt tacaagttgt ttaaaatatg
    16681 tattccgaaa ccctttaaag ttaattgtat attcatattg tgttgggtta accttatcat
    16741 ctggcaaagg taagttacgt ctatcccaaa cgtctttatc ccactcctct ccatcaaaat
    16801 aaaagttctc ataaaactcc ataaattgtt ttagattagt aacatagtag gaattagctt
    16861 ttacaggtgt tttttcttga ttagcagtaa tcttataatt agtagtggta attctaacac
    16921 cccgttttgt caaataagtt ctatactccg tcattgcttt ttcaatagga acttcagtaa
    16981 ttgaagtaat gctaggatac tttaaatcta agaaatctaa cattttatta attactgttc
    17041 cttttctaat ccagacagtt tttgcattcc atattccatt gtttaaatgg taaaaataaa
    17101 aatatttcaa ttctgttctt aaccacagat ttttaacacg ttcaaaacga acccaacgat
    17161 tccttaaagc aggattctta cttaattcta tggcagaagg atgtggacat tttcttatat
    17221 cccaactatt attagcccaa aacccctgca tttcttcatt cattacagct atttttttgc
    17281 taatctcact ctgactaata attttccttt tactagaagc attcatttct tatgctcctt
    17341 tctctcgagg tatttattaa actcattttt catatcctga tctgaaagat gaacataggt
    17401 atttaacgtt gtctgaacat gtgcgtgacc taatctcttt tgaacgaacg caacatccca
    17461 tccttcccta attagctgcg ttgcgtgagt gtggcgaagc atatgtgatg taaattctat
    17521 tccagtcctt ttaactattc ttctaactag atcaagaaca ctttggtact ttagtggttt
    17581 cccaaaatag ccttctttta aggaaataaa aacataatca tgctccaatt cctcactata
    17641 ctcatatatc aagtaatctg tataaagtga cataagttct ttactcacat gtattgttct
    17701 ttccttcctt aatttaatat aagcttcatt aacattaaca tctctaggtg ttaaatggat
    17761 ttgattgtcc caagtgacaa tatcttcaag cctaagcgat aacacttcac cgattcttaa
    17821 accaccctca tacataagca ttaaaattaa tttatctctt ttcgtatgac aagcatcaat
    17881 aatttgctta acttcctctg atctcaatgt tcttatctgt ttctttttaa cccttaactt
    17941 taagacattc ttttggtatc tacccttatt aacatgatgt aaaaatcctt tgaaatttct
    18001 tccctttgct tgtttaaata catcaattga tttaaattct cctaatctac ttaaataatc
    18061 aagaaaactc ataactacat ttaaaattgt attcactgtc gtttcttctc ttatggcttt
    18121 ttttgactga agatcaatta catttgatgc tgaaggatat ctcaaccaac ctacgaagtc
    18181 tgctaacaac tcaaagttaa tatcattaag aataacacct ctctgttcca tgaactcgta
    18241 cagcaacttt aaatgatagc agtatgcttt aatggtatt
//
LOCUS       DARBET010000053        18010 bp    DNA     linear   BCT 07-NOV-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4027
            SAMEA114569841-rid20112183.denovo.054, whole genome shotgun
            sequence.
ACCESSION   DARBET010000053 DARBET010000000
VERSION     DARBET010000053.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA114569841
            Sequence Read Archive: ERR12203666
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 18010)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 18010)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001972618.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001972618.1
            Genome Coverage       :: 132x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/07/2023 13:18:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,041
            CDSs (total)                      :: 2,975
            Genes (coding)                    :: 2,908
            CDSs (with protein)               :: 2,908
            Genes (RNA)                       :: 66
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 67
            CDSs (without protein)            :: 67
            Pseudo Genes (ambiguous residues) :: 0 of 67
            Pseudo Genes (frameshifted)       :: 26 of 67
            Pseudo Genes (incomplete)         :: 28 of 67
            Pseudo Genes (internal stop)      :: 20 of 67
            Pseudo Genes (multiple problems)  :: 6 of 67
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18010
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA114569841-rid20112183.denovo.054"
                     /isolate="VRE4027"
                     /db_xref="taxon:1352"
                     /country="Denmark"
     gene            complement(25..723)
                     /locus_tag="SCJ86_001960"
     CDS             complement(25..723)
                     /locus_tag="SCJ86_001960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287245.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 glutamine amidotransferase"
                     /protein_id="HEG1169496.1"
                     /translation="MANYELNIAHLYGNLMNTYGDNGNLLMLKYIAEKMGVSCHTEIV
                     SIHEPFDADKYDLVFFGGGQDFEQLIISKDIQKKKDSLINYIENDGVMLAICGGYQLL
                     GHYYMGANGEKIQGISALDHYTLSQENNRFIGDIVIHNEEFNETYYGFENHNGRTFLG
                     EGERPLGKVVQGQGNNGEDQSEGVIYRNVFGSYFHGPILARNENLAARLIRLALEQRY
                     GKELDLPYLEGQTA"
     gene            complement(716..2068)
                     /locus_tag="SCJ86_001961"
     CDS             complement(716..2068)
                     /locus_tag="SCJ86_001961"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343391.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Mur ligase family protein"
                     /protein_id="HEG1169497.1"
                     /translation="MGIRSHFAIAVGKTSQWALKTFFKGGSSLPGKLALKVDPHILDS
                     LAKDYQVVVVTGTNGKTLTTALTVNILRQQFDEVLTNPTGANMVQGIVSTFLQAKPKK
                     GQKKFAVLEIDEASLSKVTEYMKPELFLFTNIFRDQMDRYGEIYTTYKMIVDGAAKSP
                     NATIISNGDSPIFNSIETVNPRKYYGFDHEEDREQMAHYNTDGVLCPNCHHILHYKMI
                     TYANLGKYYCPNCDFKRPELDYRLTDMVRMDNVSADFVIDGHEYGIEVGGMYNVYNAL
                     AATAVAEYFNVDPDKIKQGLGYDEKVFGRQEVININGKKCTLVLVKNPVGINQVIDMI
                     GLAPYPFSLVALLNANYADGIDVSWIWDGNFENFADMDIPAVISGGDRHTDMTLRLKV
                     AGIPENKLTETKELPEVIEKIKELPTEHVYILATYTAVLQLRKELAAQGFIKGGMNRG
                     "
     gene            complement(2291..3691)
                     /locus_tag="SCJ86_001962"
     CDS             complement(2291..3691)
                     /locus_tag="SCJ86_001962"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629268.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TrkH family potassium uptake protein"
                     /protein_id="HEG1169498.1"
                     /translation="MHVDLFFRRLQRRGQKFAANHFSSIQIIVFYYILMTVLSLVLFY
                     MPIFREPDSHVSFVDMLFMAISTVSVTGLSTFDINSVFNDNGIILLEILFQVGGLGIM
                     MISTAFVIFSKRRITLKQRQLIMTDMNQPRLSGIVRLIRITFAILIWFQLLFGTFFSI
                     YFYYRGYFDRWRDAVFYGFYQAISAVTNSGFDVTGDSIKPFAHDYFFLILIMFLIFVG
                     GIGFPVLMECREWLLYKRSNAKLPFRFSLFTKLAVLAFVILFVSGTVLIYLLEKDHLF
                     QDSNMSVKWINSMFYSITTRNAGLQIHDLGDFQITTLIIFSLLMFIGCSPSSVGGGIR
                     TTTVAIIGLYLYSFLKSEDNINIFGRRIDQDDVRKSVVVFMLSLGMCFFCIVFLSATE
                     EQTLISIIIEVTSAFGTTGLSLGITGDLSVVGKITIATLMFIGRIGMLYTLMLFVPKE
                     TRDLGYEYPSEKIIIG"
     gene            3852..4589
                     /locus_tag="SCJ86_001963"
     CDS             3852..4589
                     /locus_tag="SCJ86_001963"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325405.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="epoxyqueuosine reductase QueH"
                     /protein_id="HEG1169499.1"
                     /translation="MINAQQIVEKMKNQKINYDTVLKKMIMQWEKEEVKPKILIHTCC
                     APCSTHTLEFMSQYAEITLFFSNSNIHPKSEYLRRLHEQKRFVDQFNEKTDHHVALIV
                     DEYRPNEFTKMVLTNHLENEKEGGARCSACFNMRLDLVAQKAQELGYDYFGSALTISP
                     KKNAALINQIGMDIQKIYGTQYLPSDFKKNKGYERSLQMCKEYDIYRQCYCGCVFAAK
                     QQGCDLKEINREAKAYLKTSTVVFEEN"
     gene            4714..5193
                     /locus_tag="SCJ86_001964"
     CDS             4714..5193
                     /locus_tag="SCJ86_001964"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="A-agglutinin attachment subunit precursor"
                     /protein_id="HEG1169500.1"
                     /translation="MRKVAPILKPIAVLTISTIVATTTGIVNSSSYRAPSSSSSTVEE
                     TTPTTSVSTSEPTTTESLNETTTTSTWDDTTTSSSYDETYTTDSSSETSNTVPDGTPS
                     DSTNPSTSPDTSASSETIPSSTTVDDNTQNDDAARTAQQNQDSGNTQTYNQQAGDSQ"
     gene            5190..5957
                     /locus_tag="SCJ86_001965"
     CDS             5190..5957
                     /locus_tag="SCJ86_001965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325406.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEG1169501.1"
                     /translation="MTFLLDLINGVHKFLGYLDISPKYLNRGYTILSVIPTLYLLRIV
                     YGLWQNQNYLQFFLYGLAFLVLVYFTVLNVFYYFLDKNTKADVTQLFVKYLPDEAFNI
                     QTEESIDNGNSIDKVNTQEVMVSYDEDYQLKLAENMRYLIGNGEIKTNELGRMDGFLV
                     DRNTLYPYYYVKKTGEKEYVLNIGRSYSDMEKVGTIRLDSTEDTLEPVGLFIVGGDFV
                     KDGLRYHEPYRLKLIAKKAQSQEEPVVYSRSQRRRHS"
     gene            6187..6756
                     /gene="lepB"
                     /locus_tag="SCJ86_001966"
     CDS             6187..6756
                     /gene="lepB"
                     /locus_tag="SCJ86_001966"
                     /EC_number="3.4.21.89"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325407.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptidase I"
                     /protein_id="HEG1169502.1"
                     /translation="MKEFLKNWGILILVLAAILLARVYVFTPVTVNGHSMDPTLSDGQ
                     RLISSKISNYERMDIITTKEPGDEERMIVKRIIGMPGDTVKMENDQLTINGKKYDEPY
                     LDEFKKEFSEDKLQGEYAYSSGFQAQAESSSTFTSDFEYTVPKGKYLVLGDNRLISKD
                     SRMFGLVDKDMIQGKVVFRYWPLSEIKIM"
     gene            complement(6860..7462)
                     /locus_tag="SCJ86_001967"
     CDS             complement(6860..7462)
                     /locus_tag="SCJ86_001967"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310111.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HEG1169503.1"
                     /translation="MTKNIFDSIANHYDSPDRQALAKIIREELTTYLPRDSHQKVLLD
                     YGGGTGLVSLPLAERFKELIIADASETMLKMAEEKIQAADLKNVRTIHADASVEFPAV
                     QANLILLSLVLLHIPDTENILTKLYEILAPGGQLIIVDFDKNEQISHPKVHNGFSQEE
                     LNNRLEKTGFVSATSHTFHRGEKLFMNKNASLFLSISQKA"
     gene            7568..8037
                     /locus_tag="SCJ86_001968"
                     /pseudo
     CDS             7568..8037
                     /locus_tag="SCJ86_001968"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325408.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
     gene            8143..8727
                     /locus_tag="SCJ86_001969"
     CDS             8143..8727
                     /locus_tag="SCJ86_001969"
                     /EC_number="2.7.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289675.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thymidine kinase"
                     /protein_id="HEG1169504.1"
                     /translation="MAQLFFKYGAMNSGKTIEILKVAHNYEEQNKPVVLMTSGIDTRD
                     GLGVVSSRIGLKRNAMPIFEETDVFDVIQKMESKPYCVLIDEAQFLKKKHVIAFTRIV
                     DELDIPVMAFGLKNDFRNELFEGSKYLLLYADKIEEMKTICWFCHKKAIMNLHYIDGN
                     PVYEGDQVQIGGNEAYYPVCRKHYFHPSMITEGE"
     gene            8730..9803
                     /gene="prfA"
                     /locus_tag="SCJ86_001970"
     CDS             8730..9803
                     /gene="prfA"
                     /locus_tag="SCJ86_001970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003128515.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor 1"
                     /protein_id="HEG1169505.1"
                     /translation="MYDQLQAIEDRYEELGELLSDPEVISDTKRFMQLSKEEANTRET
                     VEVYRRYKEVVQGITDTEELLGEKLDDEMAELAKEELSELKKEKEVLEEKIKILLLPK
                     DPNDDKNIIMEIRGAAGGDEAALFAGDLFNMYQKYAEAQGWKTEVMEANVTGIGGYKE
                     VIMMITGDNVYSKLKYESGAHRVQRVPSTESQGRIHTSTATVVVMPEAEEVEIDIEDK
                     DIRTDIYHASGAGGQHVNKTASAVRLTHLPTGIVVAMQDERSQIKNREKAMKILRARV
                     YDKIQQEAQSEYDANRKSAVGTGDRSERIRTYNFPQNRVTDHRIGLTIQKLDQILAGK
                     LDEIVDALIMYDQTSKLEAMQNG"
     gene            9796..10635
                     /gene="prmC"
                     /locus_tag="SCJ86_001971"
     CDS             9796..10635
                     /gene="prmC"
                     /locus_tag="SCJ86_001971"
                     /EC_number="2.1.1.297"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor N(5)-glutamine
                     methyltransferase"
                     /protein_id="HEG1169506.1"
                     /translation="MGKMTYREVLSRASSFLEDHGKEAYSIQFLFLERKQWKKLDWLL
                     HMNEEISEEEQRLIETDLRLLLADHPPQYLLGYADFYDHRLKVTEATLIPRPETEELV
                     EWCLDETPGVPLEVIDIGTGTGAIAISLKAARKNWHISAVDLSEEALEVAKENAQKEG
                     TKISFYHGDTLGPVMDQQFDVIISNPPYISRNEWELMDESVRSFEPKMALFAENDGLA
                     IYEKIAVEAPSVLKSNGKIFLEIGFRQGEAVKKIFQQAFPDKKVAIKKDLFGNERMIQ
                     VYG"
     gene            10628..10807
                     /locus_tag="SCJ86_001972"
     CDS             10628..10807
                     /locus_tag="SCJ86_001972"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300130.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEG1169507.1"
                     /translation="MVEVVDEKFFICFFIIKKHLFDKDVNDFLVYQQVYPLLTNRLST
                     NSGKLEDKLRQNKNV"
     gene            10829..11854
                     /locus_tag="SCJ86_001973"
     CDS             10829..11854
                     /locus_tag="SCJ86_001973"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320384.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonylcarbamoyl-AMP synthase"
                     /protein_id="HEG1169508.1"
                     /translation="METKRYDETELQEAAEALKEGELVAFPTETVYGLGANALLPNAV
                     KKVFSVKGRPQDNPLIVHVASFEQVKEYVDNFHPAAEKIVKNFWPGPLTLIFKIKKDT
                     LPSVVTGGLSTAAFRIPDNKKTLEVIELSGVPLVGPSANTSGKPSPTTADHVYHDLQG
                     KITGIIDDGATRIGVESTVLDLSDPTAMPMILRPGAVTKEQIEAVIESPVAIDQHLVK
                     ENETPKAPGMKYKHYSPDTRVLMVRDGDWSTAVQWAKNKKIRAGVIASPEIADQVRTD
                     TAAVYMYNDNSVEAAAKGLFAGLRGLDEPTLGLDLIFVQVYPETGLGNAYMNRLKKAA
                     GQNYFEK"
     gene            12029..13273
                     /locus_tag="SCJ86_001974"
     CDS             12029..13273
                     /locus_tag="SCJ86_001974"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374734.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydroxymethyltransferase"
                     /protein_id="HEG1169509.1"
                     /translation="MVDYKTFDPDLWAAIAKEEERQEHNLELIASENFVSEAVMAAQG
                     SILTNKYAEGYPGHRYYGGCEFVDIVENLAIDRAKELFGAKFANVQPHSGSQANTAAY
                     LALVEPGDTILGMDLSAGGHLTHGSPVNFSGKTYHFVAYGVDPTTEVIDYNVVRILAR
                     KHQPKLIVAGASAYGRTIDFAKFREIADEVGAKLMVDMAHIAGLVAAGLHPNPVPYAD
                     ITTTTTHKTLRGPRGGMILTNDEALAKKINSAVFPGIQGGPLEHVIAGKAVAFKEALD
                     PAFKEYSEQIIANAKAMVKVFNQAIGTRVISGATDNHLMLIDVRELGINGKEAESILD
                     SVNITVNKNSIPFETLSPFKTSGIRIGTPAITTRGFKEEDAVKVAELVVKALQAKDDN
                     AQLDEVKTGVRELTEKFPLHKK"
     gene            13426..14373
                     /locus_tag="SCJ86_001975"
     CDS             13426..14373
                     /locus_tag="SCJ86_001975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294097.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HEG1169510.1"
                     /translation="MTDRISLEKEAVAFSDANAPHPRIYELAPEEGRVLLEEVQESPV
                     NKYSVDIEDLMVDTGEWGKLNIRLVRPEGNMDKLPVIFYIHGAGWVFGSAQTHDKLIR
                     ELAVRTNSVVVFPEYSRSPEAKYPTAIEQNYAVLQQLNKLAKSKNLDLENLIVAGDSV
                     GGNMATVMTILTKERKGLPISKQLLYYPVTNADFDTDSYHEFGENYFLTREGMMWFWD
                     QYTTNEEERAEITASPLRANINELNDLPSAMILTGEADVLRDEGEAYARKLRDAGVPV
                     TQVRFQGIIHDFVMVNAMDQTKATRAAMALSTSWIKKKE"
     gene            14507..15136
                     /gene="upp"
                     /locus_tag="SCJ86_001976"
     CDS             14507..15136
                     /gene="upp"
                     /locus_tag="SCJ86_001976"
                     /EC_number="2.4.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013774406.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uracil phosphoribosyltransferase"
                     /protein_id="HEG1169511.1"
                     /translation="MGKFQVIDHPLIQHKLTIIRDKNCGTKVFREVVDEIAMLMAYEV
                     SRDMPLEDIVIETPITETTQKTLSGKKVAIIPILRAGIGMVDGILELIPAAKVGHIGL
                     YRDEETLEPHEYFVKLPEDIDARQLFVVDPMLATGGSAIMAIDALKERGASNIKFVCL
                     VAAPEGVKALQEAHPDIDIYTASLDEKLNEHGYIVPGLGDAGDRLFGTK"
     gene            complement(15275..15517)
                     /locus_tag="SCJ86_001977"
     CDS             complement(15275..15517)
                     /locus_tag="SCJ86_001977"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010719992.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEG1169512.1"
                     /translation="MKMATFGKFDTTIVPSEIGEKFKINTHVMFTIHGKSQTGTISKQ
                     LKNSAVVEIDETAENANLMTQSKGVVIVNYKELKKI"
     gene            15638..15710
                     /locus_tag="SCJ86_001978"
     tRNA            15638..15710
                     /locus_tag="SCJ86_001978"
                     /product="tRNA-Thr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:15671..15673,aa:Thr,seq:cgt)
     gene            complement(15829..16467)
                     /locus_tag="SCJ86_001979"
     CDS             complement(15829..16467)
                     /locus_tag="SCJ86_001979"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303626.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CPBP family intramembrane metalloprotease"
                     /protein_id="HEG1169513.1"
                     /translation="MNNILGKKIELKKGILGTIVVVLGLLGLLFAPSIISIFSLTITG
                     FIAFTLVFGKKDTKKMFSKPVAPVKNIAKYFFINVVISAAVSVFLQQILKWGLTGNPI
                     NEAFSPLLFIILPIMILGEELFSVYFLAIFSSKFSIPVASFLSAIIFGFIHYSTYDNG
                     NILHTVAHILLIQGVARLLFNQAAIKSNSIITSWAVHVIFDFTAILLVVLFS"
     gene            complement(16590..17222)
                     /locus_tag="SCJ86_001980"
     CDS             complement(16590..17222)
                     /locus_tag="SCJ86_001980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289236.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pentapeptide repeat-containing protein"
                     /protein_id="HEG1169514.1"
                     /translation="MIFPKVNEASLNKGSFFHIYENEDFELYNILSQKEVIENQIICH
                     PIIYKSIFKYAFFASNDFQRMEITDCIFENCDFSGCILTKSMLHRVKFVNCKLTGTKL
                     MESYIGNTLFENCKMDYVNLSGSNIKESTFEHSVLSSADFVDCSLTKIMFSTNDLENA
                     SFFDTDLKNIDLSSNSFEMIEVRSEHLKNCTLNQLQALGFARKFLQIKVV"
     gene            17573..17872
                     /locus_tag="SCJ86_001981"
     CDS             17573..17872
                     /locus_tag="SCJ86_001981"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bacteriocin immunity protein"
                     /protein_id="HEG1169515.1"
                     /translation="MSKSEEIENMVLKLYDLVLNPEIKEKERILLIDAKTGLEKGQYY
                     PKVINNLERSLRPLAIRGELSKPVSPFYMEISTIGKFEKELGRGMASAPITFGHL"
BASE COUNT         6166 a         3154 c         3558 g         5132 t
ORIGIN      
        1 gtcacattcc cctttttcga ataactaggc tgtttgtcct tcaagatagg gcagatccaa
       61 ttctttaccg taacgttgtt ctaaagctag gcggatcaaa cgagctgcca ggttctcatt
      121 tcttgcaagg ataggtccat ggaaataaga accgaagaca tttcggtaaa tgacaccttc
      181 agattggtct tcaccattat ttccttgacc ttgtaccact ttccccaaag gtcgttcacc
      241 ttcacctaag aaagtccgac cattgtggtt ttcaaatcca taataagttt cattgaattc
      301 ttcgttatgg ataacgatat caccgataaa ccggttattt tcctgactta acgtgtagtg
      361 atccagtgca ctgattcctt ggatcttttc tccattagct cccatgtagt agtgacctag
      421 caattggtag ccaccacaaa tggctaacat gacgccatca ttttcgatat agttgatcaa
      481 agaatctttc tttttctgga tgtcttttga aatgattaat tgctcaaagt cttgaccacc
      541 gccaaagaaa accaagtcat atttatcagc atcaaaaggt tcatgtatac taacgatctc
      601 cgtatggcaa gaaaccccca ttttttctgc tatatatttc aacatcaaaa ggtttccgtt
      661 atcaccatat gtgttcatca aatttccata aaggtgggcg atattgagtt cataattagc
      721 cacggttcat tcctcctttg ataaatcctt gagcagctag ttctttacga agctgcaata
      781 cagcagtgta agtagctaaa atatagacat gctcagttgg caattctttg attttttcga
      841 ttacttctgg taattctttt gtttctgtca atttattttc tggaatcccg gcaactttta
      901 gacgtaaagt catatctgta tgacggtctc ctccagaaat aacagctggg atatccatat
      961 cggcaaagtt ttcaaaatta ccatcccaaa tccagctgac atcgattccg tctgcgtaat
     1021 tggcatttaa taaagccact aaagaaaatg gataaggagc tagaccaatc atatcgatta
     1081 cttgattgat acctacagga tttttgacaa gtactaatgt acattttttc ccattgatat
     1141 tgatgacttc ttgtcggccg aatactttct cgtcatatcc taaaccttgt ttgattttat
     1201 caggatcgac attgaagtac tctgccactg cagtagctgc taaagcattg tagacattat
     1261 acataccgcc aacttcgatt ccgtattcat gaccatcgat cacgaaatct gctgacacat
     1321 tgtccatccg taccatatca gttaatcgat aatccaattc aggacgtttg aagtcacaat
     1381 ttggacagta atatttccct aagtttgcat aagtgatcat tttataatgc aagatgtgat
     1441 gacaatttgg acataagaca ccgtctgtat tgtaatgagc catttgttca cgatcttctt
     1501 catggtcaaa gccataatat tttctaggat tgactgtttc gatagaattg aagattggtg
     1561 aatcaccgtt actgataatc gttgcatttg gtgactttgc ggcaccgtct acaatcattt
     1621 tataggtcgt atagatttct ccataccgat ccatttgatc acggaaaata tttgtaaata
     1681 agaacagttc tggtttcata tattctgtga ctttgctcaa acttgcttca tctatttcta
     1741 ggacagcaaa ctttttctgt cctttttttg gttttgcctg caagaaagtc gatacaattc
     1801 cctgtaccat attcgcacca gtagggttgg ttagaacttc atcgaattgc tggcgcaaaa
     1861 tattgactgt taaagcggta gtcaatgttt taccgtttgt tccggtgaca acgacgactt
     1921 ggtagtcttt tgccaaacta tctagtatat gtggatcgac tttcagcgct agtttacctg
     1981 gtaaacttga tccgccttta aaaaaagttt ttaatgccca ttgcgacgtt tttccaactg
     2041 caatcgcaaa atgactgcgt attcccatta tgtaacctcc aaaaacgatt tgctgacgaa
     2101 taaattcaac taatcatacc atatgttgaa aaaaaggtta aattgctttt cttattctta
     2161 agaaaaggtt acggctgaaa gtgcatgaaa tcggacaaaa tcgcatgatt taacaaaaaa
     2221 acagcttgaa acaaaatgat tgtttcaagc tgctgtgatt ttattattcc ctcttaccta
     2281 tattcccatt ttaaccgata ataattttct cgcttggata ttcatagcct agatctcgtg
     2341 tttcttttgg cacgaataac atcaatgtat atagcattcc gatccgccca ataaacatca
     2401 gtgtagcaat cgtgattttg cccacaacag acaagtctcc ggtaatccct aaagataaac
     2461 cagttgttcc aaacgcagaa gttacttcga tgatgatcga gatcagcgtt tgttcttctg
     2521 tagcagataa aaaaacgata caaaagaaac acataccaag agaaagcata aagacaacga
     2581 ctgattttcg cacatcatct tgatcgattc tccgtccaaa aatattgatg ttatcttcac
     2641 ttttgaggaa ggaatataga tacaaaccga tgattgctac agtagttgtt cgaatcccgc
     2701 cgcctacaga actaggactg catccgataa acatcagtaa agaaaaaatg atcagtgttg
     2761 tgatttgaaa atcacccaaa tcgtgaatct gcagaccagc attacgagtc gtaatcgaat
     2821 aaaacatcga attgatccat ttgacactca tattggaatc ttggaataaa tggtcctttt
     2881 ccaatagata aatcaagaca gttcccgaaa caaacaagat aacaaaagct aacacagcta
     2941 atttggtaaa caaggaaaaa cgaaacggaa gcttcgcatt ggaacgtttg tataatagcc
     3001 attcccggca ctccatcaat acaggaaaac caattcctcc gacaaaaatc aaaaacatga
     3061 tcaggatcaa gaaaaaatag tcatgtgcaa agggcttgat ggaatcccca gtgacgtcaa
     3121 acccagagtt cgtgacagcc gatatcgcct gataaaaacc gtaaaaaacg gcatcacgcc
     3181 aacgatcaaa atagcctctg taataaaaat aaatagaaaa gaatgtccca aaaagtaatt
     3241 gaaaccagat taaaatagca aaagtgatcc gaatcagtcg gacgatacca gacaaacgcg
     3301 gttgattcat atccgtcatg atcagctgtc gctgtttcag tgtgatccgt cgtttagaaa
     3361 aaatgacaaa agcagtagaa atcatcatga tccccaaacc accaacttga aataatattt
     3421 ccagcagtat aataccgtta tcattgaaca cagaattgat atcaaaagtc gatagtccag
     3481 tcacactcac tgtactgatt gccatgaaca gcatgtccac aaaagaaaca tggctatctg
     3541 gttcgcgaaa aatcggcata taaaatagaa cgagtgataa gaccgtcatc aatatataat
     3601 aaaaaacgat gatttggatc gatgagaaat gattggcagc aaacttctgt cctcgccgct
     3661 gcagccttcg aaagaataaa tcaacatgca cagtactatc ctcctgacaa tagtcgtttc
     3721 taccagtata caaggggaat taggaatact caaagaaatt atcaaacaga taacaataca
     3781 gctttcgatt aaaatagaaa tggcttataa taaggaaaat cctagaaaaa agaaggagga
     3841 ggaataacaa attgattaat gctcaacaaa tcgtagaaaa aatgaaaaat caaaaaataa
     3901 attatgatac agttctgaaa aaaatgatca tgcaatggga gaaagaagaa gtaaagccta
     3961 agatattaat acatacatgc tgtgctcctt gcagtacgca cacattggaa ttcatgtctc
     4021 aatatgcaga gatcacgtta tttttttcaa attcgaatat tcatcctaaa agtgagtatt
     4081 tgcgaagatt acatgaacaa aaacgatttg tagaccaatt taatgagaaa actgaccatc
     4141 atgttgcgct aatcgttgat gaataccgtc caaatgaatt caccaaaatg gttttaacaa
     4201 accatcttga aaacgaaaaa gaaggtggtg ctcgctgcag tgcatgcttc aatatgcgat
     4261 tagatcttgt tgcgcaaaaa gcgcaagaac taggatacga ttactttgga agtgccctga
     4321 ccatctcgcc aaagaaaaat gcagctttga tcaatcagat cggaatggat atccaaaaga
     4381 tttacgggac acaatacttg ccaagtgatt tcaagaaaaa caaaggctat gaacgctctt
     4441 tgcaaatgtg caaagagtat gatatttatc gccaatgtta ttgtggttgc gtgttcgctg
     4501 ccaagcagca aggatgcgat ctgaaagaaa tcaatcggga agcaaaagct tacttaaaaa
     4561 catcaaccgt agtttttgag gaaaattaaa gaaatctttc ggaaaaacgt aagaaaaaaa
     4621 taaagaattt atggtagaaa taaggtagac tgtataattt gtccgtagta aagtaaggaa
     4681 acaagaataa atttcaatga aggaagagat aagatgcgaa aagtagcacc tattttgaag
     4741 ccaatcgctg tattaacaat ctcgacgatt gtcgcgacta ctacaggtat tgtgaatagt
     4801 tccagctacc gtgcaccaag ttcaagttct agtactgtag aagaaacaac accaactact
     4861 tcagtctcga catctgaacc aacaacaacg gaaagtttga atgaaaccac tacgacaagt
     4921 acatgggacg atacaacaac tagttcgtcg tatgatgaga catatacaac tgacagctca
     4981 agcgaaacaa gtaatacggt accagatggc actccatcgg attcaaccaa tccaagtaca
     5041 agtccggata caagtgctag ctccgaaacg ataccaagca gtacgactgt agatgataac
     5101 acgcaaaatg atgatgcagc acgtacagca cagcaaaacc aagattcagg aaatacccaa
     5161 acgtataacc agcaagcagg tgattctcaa tgacattttt actagacctt atcaacgggg
     5221 tgcataaatt ccttggctat ttagacatca gtcctaaata tcttaaccgt ggatatacga
     5281 ttttaagtgt gattcccacg ctttatttat tgagaattgt ttatggactt tggcaaaacc
     5341 aaaactatct tcaatttttc ttatatggac ttgcatttct agtgcttgtt tattttactg
     5401 ttttaaatgt attttattac tttttagata aaaacacgaa agccgatgtc actcagcttt
     5461 ttgtgaagta tttacctgat gaagcgttca acatccagac agaagaatcg atagataatg
     5521 gaaattcgat cgataaagtg aatacacaag aggtcatggt ttcttatgac gaagattatc
     5581 aattgaaatt agccgagaat atgcggtatt tgattggaaa tggtgagatc aaaacaaatg
     5641 aactcggtcg catggatgga tttctggtcg atagaaatac attgtatcct tattactatg
     5701 tcaagaaaac aggagagaaa gagtatgttc taaatattgg acgaagctac tcggacatgg
     5761 aaaaagtagg gaccattcga cttgactcaa cagaagacac attggaacct gtcggattgt
     5821 tcatcgtcgg cggtgatttt gtcaaagacg ggttacgcta tcatgagcct tatcgtctga
     5881 aattgattgc aaaaaaggca cagtcgcagg aagaaccagt ggtttatagt cgtagccaaa
     5941 gaagaagaca ttcataatgt gatgaaacgc cgtgtatcag tggttgcgat aaaaacgttt
     6001 attcggaata agggggatgt gacacgtctt ttgtcacgtt ccttttttat ataacgagaa
     6061 agataagacg aacacatcac ttattctttc ctttcattta taaacattgt agaataaaga
     6121 aagtacaacg aagtattgtg aaaaaacgta ataaataacg aggaaaatac aagtaggagg
     6181 taagctgtga aagaattttt gaaaaattgg gggattttga tccttgtatt agcggctatt
     6241 ttattagcta gagtgtacgt gtttacacca gttactgtaa acgggcattc gatggaccct
     6301 acattaagtg atggacaacg attgatttca tctaagatct caaactatga acgaatggat
     6361 attattacta caaaggaacc gggggatgaa gaacggatga ttgttaaacg gatcatcggt
     6421 atgcctggag atacagtcaa gatggaaaat gaccagttga ccattaatgg caaaaagtat
     6481 gatgaacctt atctggatga gttcaaaaaa gaattttcag aagataagtt acaaggagaa
     6541 tatgcttata gctcaggatt ccaagcacaa gcagaaagtt catccacctt tacgagtgac
     6601 tttgaatata ctgtgccaaa aggaaagtat cttgttctag gcgataaccg cttgatttca
     6661 aaagacagcc gaatgtttgg gttagtcgac aaagatatga tccaaggaaa agttgttttc
     6721 cgttattggc ctctttcaga aattaaaatc atgtgatgaa aaaataaaaa gaagctgtga
     6781 catgtttttt gtcacagctt ctttttccga aaattccttc ttatttcttg gagttgctca
     6841 ctttttttgc aatctcattt taagcttttt ggctgatgga taagaaaagc gaagcgtttt
     6901 tgttcataaa tagcttttcc ccacgatgga aggtatgact agtagcagag acaaagcccg
     6961 ttttttccaa tcgattattc aattcttctt ggctaaagcc attatggact ttcggatgac
     7021 taatttgttc attcttgtca aagtctacga tgatcaattg accgccagga gctaaaattt
     7081 cataaagttt tgtaagaata ttctccgtat caggaatgtg taaaagtaca agtgatagaa
     7141 ggatgagatt agcttggaca gcaggaaatt cgacagaagc atcagcgtgg atcgttcgga
     7201 catttttcag atctgctgct tggatctttt cttctgccat tttcagcata gtttctgaag
     7261 catcggcaat gatcagttct ttgaatcttt ccgcaagcgg taaactaaca agcccagttc
     7321 cgcctccgta atccagcagg actttttggt ggctgtcgcg cggtagataa gtagttagtt
     7381 cttcgcgaat aatcttggct aatgcttgac ggtcagggga atcataatgg ttagcgatgg
     7441 aatcaaaaat attttttgtc atcaaatagc tccttacgta aaactttgaa attttaactt
     7501 attatagcac gtgaaaagaa agaaagcatg ctatactcaa ttcaaaaagg gagaatctag
     7561 aaagaatatg ccgatatcgt ttgtaaaaga tagagaggaa aaaggaaagt gtgtccgaga
     7621 aatattacta gacttgcctg agtggtttgg gcttcctgaa tcaacggaaa aatatatcga
     7681 ggaatcaagt aaactacctt tatggtgtga aaaaagaaaa gaagaatatt taggctttat
     7741 cactctttca cagacaagtg aagatactgc cgaaatctat tctattgtat gggaataaaa
     7801 aaagactatc atcaccaagg attaggaaca aaacttttta aagaagcaga aggttacgca
     7861 gcaaaacatt acaaatattt gcaagtgaag actgtagacg aggggcacta ctctatatat
     7921 gatcaaacta tttgtttcta cgaatcatta ggattttcaa ggttggaagt ttttccaaat
     7981 ttatgggatg aatggaaccc atgtttagtt cttgtcaaaa aattggaaca aaagtgagca
     8041 atagatttaa tactaatctg tgcttgatga aaattctcac tgtaaaaaat gctgaaaatc
     8101 aagtataata ccataggaac aaactggaag gtgaaaaaga gcatggcaca actatttttt
     8161 aagtatggcg caatgaatag cggcaagacg atcgagatcc taaaagtcgc acataattat
     8221 gaagaacaaa ataagccagt tgtcttaatg acaagcggta ttgatacaag agatggttta
     8281 ggcgttgttt caagccgaat cggactgaaa cgtaatgcga tgccgatatt tgaagaaaca
     8341 gacgtatttg acgtaattca gaaaatggaa agcaaaccct attgtgtgct gatcgatgaa
     8401 gcgcaattct taaaaaagaa acatgtcatt gcttttacga gaattgtgga cgaattggat
     8461 attccggtta tggcatttgg tttgaaaaat gattttcgaa acgagttatt tgaaggatca
     8521 aaatatttat tgctttatgc agacaaaatc gaagaaatga aaacaatctg ttggttctgc
     8581 cacaaaaaag caatcatgaa tttacattat attgatggaa accctgttta tgaaggcgat
     8641 caagtgcaaa ttggcggaaa tgaagcttat taccccgttt gtcgaaaaca ttactttcac
     8701 ccatcaatga ttacagaagg agaataagca tgtacgatca attacaagca attgaagacc
     8761 gttatgaaga gcttggagaa cttttaagtg acccagaagt gatttctgat acaaagcgtt
     8821 ttatgcagct ttcaaaagaa gaagcaaaca cgcgcgaaac agtcgaagtt tatcgtcgtt
     8881 ataaagaagt agtacaagga atcaccgata cagaagaact gcttggtgaa aaactggatg
     8941 atgaaatggc tgaactagca aaagaagaat tatctgaatt aaaaaaagaa aaagaagtct
     9001 tagaagaaaa aatcaagatc ttgcttttac caaaagatcc aaatgacgat aaaaatatca
     9061 tcatggaaat ccgtggagca gcaggcggag acgaagcagc attattcgct ggcgatcttt
     9121 tcaatatgta ccaaaagtat gctgaagcac aaggctggaa aacagaagtg atggaagcta
     9181 acgttaccgg aatcggtgga tataaagaag tgatcatgat gatcaccgga gacaacgtat
     9241 attcgaaatt aaaatatgaa agtggtgcgc accgcgtcca gcgtgttcct tcaactgaat
     9301 cgcaagggcg tatccatact tccaccgcaa cagttgtcgt gatgccagaa gctgaagaag
     9361 tagagatcga tattgaagac aaagatatcc gtacagatat ctatcatgct tcaggtgctg
     9421 gcggacagca cgtcaataaa acagcatctg ctgtacgttt gactcactta ccaacaggga
     9481 tcgttgttgc tatgcaagat gaacgttctc aaatcaagaa ccgtgaaaaa gcaatgaaaa
     9541 tcttgcgtgc tcgtgtgtac gataagatcc agcaagaagc acaaagcgag tatgatgcca
     9601 atcgaaaatc agcagtcgga actggggatc gttcagaacg gatccggacg tataatttcc
     9661 cgcaaaatcg tgtgactgat catcgtatcg gattaactat ccaaaaatta gatcagattt
     9721 tagcagggaa attagatgag atcgttgatg cattgatcat gtatgatcaa acatcaaaac
     9781 tggaagctat gcaaaatggg taaaatgacc tatcgagaag tcctgtcacg ggcttcttct
     9841 tttttagagg atcatgggaa agaagcatat agtatccaat ttttattttt ggaacgaaaa
     9901 caatggaaaa aacttgattg gctccttcat atgaacgaag agatttcaga agaagaacag
     9961 cggttgatcg aaaccgatct gcgacttctg cttgcagatc atccacctca gtatttgcta
    10021 ggatatgcag atttttatga tcatcggtta aaggtaactg aagcaacact gatccctcga
    10081 ccagaaacag aggagttagt cgaatggtgt ttagacgaga cgcctggtgt accgttagaa
    10141 gtgatagata ttgggacagg gactggagca attgctatta gtttgaaagc agcaagaaaa
    10201 aattggcata tatctgctgt ggatctttca gaagaagcat tagaagtagc gaaagaaaat
    10261 gctcagaaag aaggaacgaa aatcagtttt tatcatggag atacattagg accagtaatg
    10321 gatcagcaat ttgatgtgat tatttctaat ccgccgtaca tcagtcggaa tgaatgggaa
    10381 ctgatggatg aaagtgtccg gtcattcgaa cctaaaatgg ccctttttgc tgagaacgat
    10441 ggattagcta tttatgaaaa aatagcagtt gaagcaccaa gcgtactaaa atcaaatgga
    10501 aagatttttt tagaaatcgg atttcgccaa ggtgaagcag taaaaaaaat atttcagcaa
    10561 gcatttccag ataaaaaagt ggcgataaaa aaggatcttt ttggcaatga acgaatgatc
    10621 caagtttatg gttgaagttg tggatgaaaa attttttatt tgttttttta ttataaaaaa
    10681 acacttattt gacaaggatg taaacgactt tttggtttat caacaagttt atccactatt
    10741 gacaaataga ttatccacaa acagtggaaa acttgaggat aaactcagac aaaataagaa
    10801 tgtatgaaaa aggggttgtg gataaactgt ggaaacgaaa cgatatgatg aaacagaatt
    10861 gcaagaggca gccgaagcac tgaaagaagg agaacttgtt gcttttccta ccgaaacagt
    10921 atacggattg ggcgccaacg ctcttttacc aaacgctgtc aagaaagtgt tttctgtaaa
    10981 agggagaccg caagataatc cattaatcgt ccatgtcgcc tcttttgaac aagtaaaaga
    11041 gtatgtggac aactttcatc cggcagcaga gaagattgtg aagaacttct ggcctggacc
    11101 attaactttg atatttaaga tcaaaaaaga cactttgccc tcagttgtga cgggaggatt
    11161 atccacagct gcatttcgta taccggataa caaaaaaaca ctagaagtaa tcgaactttc
    11221 cggtgttccg cttgtcggtc caagtgcaaa tacgtcggga aaaccaagtc caacgactgc
    11281 agatcatgta tatcatgatc tacaaggaaa aattaccggg attattgatg atggagctac
    11341 gcgaattggt gtagaatcca ctgttcttga tttaagtgat ccaacagcca tgcctatgat
    11401 tttacgacca ggtgcagtaa cgaaagaaca aatcgaagca gtcatcgaat ctcctgttgc
    11461 tattgatcag catcttgtaa aagaaaatga aacaccaaaa gcccctggga tgaaatacaa
    11521 acactactca cccgatacac gtgttctgat ggtcagagat ggcgattggt caactgcagt
    11581 tcaatgggca aaaaataaga agatccgtgc aggtgtcata gcaagccccg aaattgcgga
    11641 tcaagtgcgg acagatactg cagctgttta tatgtataac gacaactcag tagaagcggc
    11701 ggctaaagga ttgtttgcag gtctaagagg attagatgaa ccaacacttg gattagattt
    11761 gatttttgta caagtgtatc cagaaactgg attggggaat gcttacatga atcgcttgaa
    11821 aaaagcagca ggacaaaatt attttgaaaa atgatcaatt ggttattgtc gcattctttt
    11881 agtatgatac aatgctatca agtaaatctt ctatcagtga acgaaaaact aagcgtaact
    11941 aatcgtaata gaagagatac gaatatttgg caatttgttt ttgatgggaa aaataaaatt
    12001 gtaggaaaat aaaaaggagc tgttgtttgt ggtagattac aaaacgtttg accctgatct
    12061 ttgggctgcg atcgccaaag aagaagaaag acaagaacat aatttggagc tgattgcctc
    12121 tgaaaacttt gtctccgaag cagtgatggc tgcacaagga agtatcttga caaataaata
    12181 cgcagaaggt tatccagggc atcgctatta tggtgggtgt gaatttgtcg atatcgttga
    12241 aaatttagcg attgaccgtg caaaagaatt attcggagcg aaatttgcga acgtccagcc
    12301 tcactccggc tctcaagcca acacggctgc ttatctcgct ttggtagaac caggggacac
    12361 gatcttagga atggatcttt ccgcaggcgg acatctaaca catggatcac cagtcaactt
    12421 cagtggcaaa acctatcact ttgttgctta cggtgttgat ccgacaacag aagttattga
    12481 ttataatgtg gtacgtattt tagctagaaa acatcaacca aaattgatcg tagcaggtgc
    12541 cagtgcttac ggacgaacga tcgactttgc gaaattcaga gaaatcgcag atgaagtagg
    12601 cgcaaaactg atggtagata tggcacatat cgcaggatta gtagcagcag gacttcatcc
    12661 taatccagtt ccttatgcag atatcacaac gactaccact cataaaacat tgcgtggccc
    12721 tcgaggcggc atgattttga caaacgatga agcattagca aaaaaaataa atagtgcggt
    12781 atttcctggt attcaaggcg gccctctaga gcatgtgatt gcgggtaaag cagtagcatt
    12841 caaagaagca ttagatcctg catttaaaga atatagtgaa caaatcatcg ccaatgcaaa
    12901 agcaatggtc aaagttttca atcaagcaat tggaaccaga gtcatttcag gggcgacaga
    12961 taatcatctg atgttgatcg atgttcgcga gttaggtata aatggaaaag aagcagagag
    13021 cattttggac agtgtgaata tcactgtaaa taaaaactcg attccttttg aaacactaag
    13081 tccattcaaa acaagcggta ttcgaatcgg cacgccagca atcactactc gcgggttcaa
    13141 agaagaagat gctgtaaaag tggctgaact agttgtcaaa gcattacaag caaaagatga
    13201 taacgctcaa ctagacgaag tcaaaacagg tgtacgcgaa ttaactgaaa aatttccgtt
    13261 gcataaaaaa taaaaattta aattccatta gtaaacagaa aagttcaatg ttgggctttt
    13321 ctgtttattt tttgggaaaa cggattcttt gacaatatcg gtgatgctct ctagtatatg
    13381 aggtgtaatt ttagtcttca cactaaatct cagggaggaa aagtcatgac tgatcgtatc
    13441 tcgttagaaa aggaagcagt agcattcagt gatgcaaatg cgcctcatcc gcgtatctat
    13501 gaactagcac cggaagaagg aagagtttta ctagaagaag tacaagaatc acctgtgaac
    13561 aaatattccg tcgatattga agacttgatg gtggatactg gcgaatgggg aaaactcaat
    13621 attcgtctcg tacgaccaga agggaatatg gataaattac cagttatttt ttatattcat
    13681 ggagcaggct gggtttttgg cagtgctcaa acccatgata aattgatcag agaacttgcg
    13741 gttcgtacga attccgtcgt tgtctttcca gaatatagtc gatcaccaga agcaaaatat
    13801 ccaacagcaa ttgaacaaaa ttatgctgtg ttgcaacagc tgaataaatt agccaagtca
    13861 aaaaatctgg atctcgagaa tctaatcgtt gcaggagatt cagtaggtgg taacatggct
    13921 acagtcatga cgatcttaac aaaagaacga aagggcctcc caatatccaa gcaattgctg
    13981 tattatccag tcacaaacgc tgattttgat acagattcct atcatgaatt cggagaaaac
    14041 tattttttga caagagaagg aatgatgtgg ttttgggacc aatatacgac gaatgaagaa
    14101 gagcgtgctg aaatcacagc ttcgcctttg agagcaaata taaatgaact aaatgatctt
    14161 ccttcagcaa tgattttaac gggagaagcg gatgtattaa gagatgaagg tgaggcttat
    14221 gcgcgcaaac ttcgtgatgc aggggttcct gttacacaag tacgcttcca aggaatcatc
    14281 catgattttg tcatggtaaa tgctatggat cagacaaagg caacgcgtgc agcaatggca
    14341 ttatctactt cttggatcaa gaaaaaagaa taagctttgc ttgtccgcag gaaaacttct
    14401 tttcaaaaac ggattttctt aggaaaaatg gcgagtttga ctagtcaatc acttgatttt
    14461 gttttacaat taaaaagaaa cgacagaaca aaggagagct taaaaaatgg gcaaatttca
    14521 agtgatcgat catccattga ttcaacacaa actaactatt attcgggaca aaaactgcgg
    14581 aacaaaggtt tttcgtgaag tagtcgatga aatcgctatg ttgatggcat atgaagtatc
    14641 acgggatatg ccattggaag acatcgtgat cgaaacaccg atcactgaaa caacacaaaa
    14701 aacgttgtct ggtaaaaaag tagcgattat tccaatcttg cgcgcaggta ttggaatggt
    14761 tgacggtatc ttggaattga ttccagctgc taaagtggga catatcggtc tttatcgaga
    14821 tgaagaaaca ttggaaccac atgaatattt cgtcaaactt ccagaagaca ttgatgcacg
    14881 tcagttgttt gtagtagatc caatgttggc aacaggcgga tcagcaatca tggcaatcga
    14941 tgcattgaaa gaacgtggcg cgtcgaacat taaatttgtt tgtttggtag cagcacctga
    15001 gggagtaaaa gctttgcaag aagcgcatcc tgatattgac atctatacag cttctctaga
    15061 tgaaaaattg aatgaacatg gttatatcgt tcctggacta ggggatgcgg gtgaccgttt
    15121 gtttggtaca aaataagtaa atacgaaaaa gggtgtgaca caacttttgt cacatccttt
    15181 tttggtcatt tttaacgttt gatgcagaac agctttttca cagctttcgt ttgcatataa
    15241 aaaaaccatc ccaaaaatgc agagatggtc aaattcaaat cttttttagt tctttgtagt
    15301 taacgattac tacaccctta ctttgcgtca tcaagttcgc attttctgca gtttcatcaa
    15361 tctcaacaac agcggaattt ttcaattgct ttgaaatcgt tccagtttgc gacttgccgt
    15421 gaattgtgaa cattacgtga gtattgatct tgaatttttc accaatttcc gatggtacga
    15481 tagttgtatc aaattttcca aaagttgcca ttttcataaa acctgccttt cacatcaatt
    15541 ataccacgtt ttatgaaaaa tatcatgaaa ttaaactaat tttccatcgg actctttaca
    15601 atcagccaaa tagagtatat aatttctttt ggataaggcc agcttagctc agttggtaga
    15661 gcaacgcact cgtaacgcgt aggtcgtagg ttcgatccct atagctggca ttaaaagaga
    15721 agacagatat atcaaggatt tttaatccat tgatacatct gtctttttgt ttaaaggaaa
    15781 taaagtatgc tttcttagca atagagcatg tattatttct ataaaaattt aggagaataa
    15841 aacgactagc aatatagctg tgaaatcaaa tatcacatga actgcccaac ttgtaatgat
    15901 cgagttgctt ttaatggcag cttggttgaa taacaatctt gccacccctt gtatcaaaag
    15961 gatgtgtgct acagtgtgta aaatatttcc attatcataa gtactgtaat ggataaatcc
    16021 aaagatgatt gctgacaaga aactagcgac aggaatgctg aacttactag aaaaaatagc
    16081 gagaaaatac actgagaaca attcttctcc aagtatcatg ataggcaaaa taataaataa
    16141 taacgggctg aaagcctcat ttattggatt gcctgttaaa ccccatttca aaatttgttg
    16201 caaaaatacc gagactgcgg cactgattac tacattgata aaaaaatatt tggctatatt
    16261 tttgacagga gcgacaggtt tcgagaacat ttttttcgta tcttttttac caaaaacaag
    16321 tgtaaatgct ataaatccag tgatagttaa agaaaaaatg ctaataatgg atggcgcaaa
    16381 tagcaaccct aacaaaccga gtacaacgac aatagtgccc aatatccctt ttttaagttc
    16441 tatttttttt cctaaaatgt tattcatgat aataactcct actattcagt ttttgaagat
    16501 agagtcagta tacatgaaga gttaaaatag gcaatgtttt tttatggata tttgtattgc
    16561 taaaaattta tcttatatat tcattttgat tatactactt ttatttgtaa aaattttcta
    16621 gcaaaaccaa gtgcttgcaa ctgattaagc gtacaatttt ttagatgttc cgagcgtact
    16681 tcaatcattt caaaggagtt gctacttaaa tcgatatttt ttaaatcagt atcaaaaaag
    16741 ctagcatttt ctaaatcatt agtagaaaac attattttag tgagggaaca gtctacaaaa
    16801 tctgcgcttg ataagacaga gtgctcaaag gtactttctt ttatgtttga accagataaa
    16861 tttacatagt ccattttaca attctcgaat aacgtgttcc caatataact ttccatcaat
    16921 ttagtacctg ttaatttaca attgacaaat ttcacgcgat gcaacatgct ttttgtcaat
    16981 atacagcctg aaaaatcaca gttttcgaat atacagtcgg taatctccat tctttgaaaa
    17041 tcattggaag caaaaaatgc atacttgaaa atagatttgt aaataattgg atggcagatt
    17101 atttggtttt cgatgacctc tttttgtgaa aggatattat acagttcaaa atcttcgttt
    17161 tcatagatat gaaaaaagga tcctttattc aagcttgctt cattaacttt aggaaaaatc
    17221 atatttaatt acctccattt tcataaagtc atattaacaa aagctaaacc aacttacatt
    17281 agcagaaaat gagataggtt ttcgcctttt tcgataacga tagtgtcgct atctcttgaa
    17341 acgttcttag aattgccctg agagacacaa attaggcgta attctaataa aaatcttaga
    17401 ataaaaattt ttactgattt aatttagaat gtttttaaaa taaaaatcgt tgccataaca
    17461 gtgtattgaa aggttttaac aatccagtaa tgatttaaaa aaatctagat gtagcgtata
    17521 ctttgtttat agaatgtagt gggaagaaat atatttttgg gaggaaatcg tgatgtctaa
    17581 aagtgaagaa atagaaaaca tggtactaaa attatatgat ctcgttttaa atccagagat
    17641 caaagaaaag gaacgtatac ttttgataga cgcaaagaca ggcttagaaa aaggtcagta
    17701 ttatcctaaa gtcataaata atttggaacg tagtttacgt ccgttagcaa tcaggggaga
    17761 attgtctaaa ccagtatcac cgttttatat ggagatatca acaataggaa aatttgaaaa
    17821 agaattaggt cgaggaatgg cttcggcacc gattactttt gggcatttat aaaaacgagt
    17881 tattcatctc aatgaattaa aaagtatagt caatctcata atgcaggcta ggttcattta
    17941 tttgaacaga gtctgtattt ttttattcta aataaagaaa cagatgtttc tttatattgt
    18001 attttatcct
//
LOCUS       DARBET010000054        17139 bp    DNA     linear   BCT 07-NOV-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4027
            SAMEA114569841-rid20112183.denovo.055, whole genome shotgun
            sequence.
ACCESSION   DARBET010000054 DARBET010000000
VERSION     DARBET010000054.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA114569841
            Sequence Read Archive: ERR12203666
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 17139)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 17139)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001972618.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001972618.1
            Genome Coverage       :: 132x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/07/2023 13:18:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,041
            CDSs (total)                      :: 2,975
            Genes (coding)                    :: 2,908
            CDSs (with protein)               :: 2,908
            Genes (RNA)                       :: 66
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 67
            CDSs (without protein)            :: 67
            Pseudo Genes (ambiguous residues) :: 0 of 67
            Pseudo Genes (frameshifted)       :: 26 of 67
            Pseudo Genes (incomplete)         :: 28 of 67
            Pseudo Genes (internal stop)      :: 20 of 67
            Pseudo Genes (multiple problems)  :: 6 of 67
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17139
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA114569841-rid20112183.denovo.055"
                     /isolate="VRE4027"
                     /db_xref="taxon:1352"
                     /country="Denmark"
     gene            complement(<1..626)
                     /locus_tag="SCJ86_001982"
     CDS             complement(<1..626)
                     /locus_tag="SCJ86_001982"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HEG1169516.1"
                     /translation="MDNHTRKLLGLTDKHLFFDEEWLIEKEYKGVQAQFIKGKLDDSS
                     SYCEHCRSVRIIRNGSYTTRTQMLKVKEKLTILELKRTRFLCHDCGRTFSAKTDLVDE
                     HHQLTKELKQVILMELYENQSRKLIAKKYFVSDGTVTRILREATKHYQPRMNFLPTVL
                     CMDEFKSMNSVSGSMSFICVDGTTNQLFTILEDRRLYKLTQYFLRFPR"
     gene            899..3370
                     /locus_tag="SCJ86_001983"
     CDS             899..3370
                     /locus_tag="SCJ86_001983"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation-translocating P-type ATPase"
                     /protein_id="HEG1169517.1"
                     /translation="MKNKWINRSKKKKVTQRSSDKVIRLFPSYKEGLTADQVKERIEK
                     GAANNSVDPTFKTNQQIVLENIFTYFNLIFLILAILLCLVESYKNLTFLPVIIANTGI
                     AIFQEIRSKKILDELNVLNTAKVNVIRDGIEQTIDIEDLVIDDIVILETGYQIPADAE
                     VVEGKLQVNEALLTGEADEITKEIGDPLMSGSFIVSGKAYARLDKVGADSYISQLTVK
                     AKTMGEGEQSEMIASLNQLIKWIGIIIIPIGILLFSQSYFFNGNTIKESVVAMEAALI
                     GMIPEGLYLLTTIALALSATKLAKQRVLLHNMKSIETLARVNVLCVDKTGTITENKMS
                     VQKMIVSKKQEIISQSDQLEACIADYAKAMAGDNATMEAVKAYFTKTTDQSYHHVIPF
                     SSVQKFSSVALADKVYVLGAPEMVLRDQLPEYAEEFVSFAEKGYRVLVFGVYDGILEE
                     PVLTEAVTPLGYILIANPIRKEARATFDYFKKQQVAIKVISGDNPLTVSNVAVQAGIS
                     HADQYVDASKLSEEEYEAAVEKYTVFGRVKPEQKKIFVQLIKRNNTVAMTGDGVNDIL
                     AMKEADCSIAMASGNEAAMQASQVVLLDSDFSRMPEVVAEGRRVVNNIERSASLFLVK
                     NIFSFLLSLFSVIFALTYPLEPSQITLISLFTIGLPSFLLALEENKKRIRGKFIMNVM
                     EKAVPGGLTDMIVVGALVICGVTLNLNKTDVSTASTMLLIAVGFLVLYKICSPLNKFR
                     SQIILFCASGNFFSVIFLHKLFSITGISAVSLLLLSILFFSADSIFRHLTTLVEYLFE
                     RKNEKEQKPFSRVAKKFLSIFKREN"
     gene            complement(3473..5209)
                     /locus_tag="SCJ86_001984"
     CDS             complement(3473..5209)
                     /locus_tag="SCJ86_001984"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629312.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component sensor histidine kinase"
                     /protein_id="HEG1169518.1"
                     /translation="MKWKKIYERLFFLILLVSLFFGGWQLISHYFQQQIVEQQESYLT
                     KKAQLLIRQLDVENLQAAQNKTALEEFVHQSNERITLMDATGKILFDTNNETLNEQRN
                     SRPEIKAVLNGGNLGSALRKSTTLNEDLLYVALPVKKSGQLEAILRIAEPTSGFLPRT
                     ESFRRWVFFFFLSFFLVLAGMIYYLIYQKNQPLKTVLPVLKKMVQNPNQAEVIMQTPD
                     QWEELYQTINALSEQMSKMYRACTTTEEQLYTLLNELMIGVFLIDDADSRLLLLNPKM
                     QLHLNVVSYQPEQRYTEVIQEPKLIQLIHQVTPEHPLIHQEITLTEGRQTLDLSLRYF
                     TNSEGTGQILGVAYDLTKVRRLEKLQKDFVGNVSHELKTPVTSLIGFTETLLDGAKDD
                     PQTLTSFLEIMQKDAIRLDKLIREIIQLSKDGENSYEIQTIYTEPYFQQIVQNYQPII
                     EKKRLTIRLIGKNEPFTTKTDILYPIIKNLIENAVQYSKSDSEIIIRYQATDDLSFSV
                     QDFGIGIDIEDQERIFERFYRVDKARSRHSGGTGLGLAIVKDYVQLLNGTITVDSHLG
                     TGSTFTVTIPKM"
     gene            complement(5206..5910)
                     /locus_tag="SCJ86_001985"
     CDS             complement(5206..5910)
                     /locus_tag="SCJ86_001985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HEG1169519.1"
                     /translation="MKKILVVDDEPSIVTLLTFNLEKEGYKVTSATDGGEGLELALEQ
                     SFDFIILDVMLPTMDGMEITQRLRQEKNETPILMLTAKDDQVDRIIGLEIGADDYLTK
                     PFSPREVLARMKAIFRRIEPRKQSKDEAEPEYLSIGQITADLTNYQVTVDDHLIELTP
                     KEFELLVYFMKRKDRVIDRDTLLDRIWNFDFAGQSRIVDVHVSHLREKIERDPKHPKY
                     LVTIRGFGYKFQEPKR"
     gene            6041..6544
                     /locus_tag="SCJ86_001986"
     CDS             6041..6544
                     /locus_tag="SCJ86_001986"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327984.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF523 domain-containing protein"
                     /protein_id="HEG1169520.1"
                     /translation="MIGISACLGGLACRYDGQKKEVPELMELVETGEAVMVCPEVIGG
                     LPIPRDPAEIIGGNGFDVWKDQAKVWTTSGEDVTEAYKNGAIKAYQKLQEKHIDLVIM
                     KEKSPSCGTHSIYDGSFSGIKKNGAGVATAYFIQQKMTVLSDEEWLKTRGEQNGNRKD
                     QSNERAL"
     gene            6504..6842
                     /locus_tag="SCJ86_001987"
     CDS             6504..6842
                     /locus_tag="SCJ86_001987"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332522.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative DNA-binding protein"
                     /protein_id="HEG1169521.1"
                     /translation="MEIEKTNRMNALFEFYSTLLTEKQMNYMELYYADDFSLGEIAEE
                     YEVSRQAVYDNIKRTSKILEDYEKKLHLFSNYIVREQVLENMTAYITDKYPEDKKLLE
                     YVQQIQAIEE"
     gene            6863..8281
                     /gene="ffh"
                     /locus_tag="SCJ86_001988"
     CDS             6863..8281
                     /gene="ffh"
                     /locus_tag="SCJ86_001988"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="signal recognition particle protein"
                     /protein_id="HEG1169522.1"
                     /translation="MAFESLTERLQQAMGKIRKKGKVSEADVKEMMREIRLALLEADV
                     NLQVVKDFTKRVRERAVGAEVLESLSPAQQIVKIVDEELTITLGSETAELNKSPKIPT
                     VIMMAGLQGAGKTTFTGKLANYLKKNENARPLLIAGDVYRPAAIDQLKVLGQQLDVPV
                     FDMGTEVSPVEIVRQGMELAKEKKNDYVLIDTAGRLHIDETLMDELKQIKELTQPNEI
                     LLVVDAMTGQDAVNVADSFNQQLGITGVVITKLDGDTRGGAALSIRSVTGAPIKFIGS
                     GEKLTDLEVFHPDRMASRILGMGDMLTLIEKAQQDYDEKKAEELAQKMRENSFDFNDF
                     IEQLDQVMGMGPLEDLIKMIPGMNQVPGIENVKVDPKDVERKKAMVYSMTPAERANPD
                     LLNPSRRRRIAAGSGNSVVEVNRMIKQFKESRKMMQQMSKGDMNIPGMDQMFGSGVKG
                     KLGKMAMNRMIKKNKKKKKKRK"
     gene            8393..8971
                     /locus_tag="SCJ86_001989"
     CDS             8393..8971
                     /locus_tag="SCJ86_001989"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306269.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-3-methyladenine glycosylase I"
                     /protein_id="HEG1169523.1"
                     /translation="MRCPWSEQTESMRQYHDTVWGVPEYDDQKLFRKLMLDINQAGLS
                     WQTILNKSEAFDEAYDRFDIKKVANYGETKISELMENKGIIRNRRKIEAAVQNAKAVL
                     KIQEEIGSFSEYLWSFSNGKIVDSSYHSQEEVPTTSPLSDELAKDLKKRGFKFIGSTT
                     IYAFLEAVGIINDHLDSCFRKEEVVRIGRKGE"
     gene            8975..9496
                     /locus_tag="SCJ86_001990"
     CDS             8975..9496
                     /locus_tag="SCJ86_001990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329440.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrofolate reductase"
                     /protein_id="HEG1169524.1"
                     /translation="MRKIIFYGAISLDGYLATKTDDLQWLFDTPTGKQTTYEAFYETI
                     DTTIMGRKTYQEAKKYLETEAIYPEKKNFVFSTHAQLQLPDAEVVNEDPVAFLKNLQM
                     TEGKDIWVVGGGRLIKPLLEHQMIDEWYIQITPVLLGDGIPLFQKGTYEARFEMLDTT
                     RFGEFIELHYVRK"
     gene            complement(9575..10129)
                     /locus_tag="SCJ86_001991"
     CDS             complement(9575..10129)
                     /locus_tag="SCJ86_001991"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325028.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent oxidoreductase"
                     /protein_id="HEG1169525.1"
                     /translation="MTKKIGFFIGSLRKDSYNKKVAEAFAKLLPEGYESVFVKIDDLP
                     FYNEDLETPEQAPAEWTRFREEVRGLDGVVFVTPEYNRSVPAVLKNALDVGSRPYGHS
                     VWDHKPGLVVTASPGGIGGFGANHHLRQSLVFLNVPTLQQPEAYIGNVANLIDENGNI
                     VEGTLSFFQTILDAYLDFSNKLTE"
     gene            10382..10657
                     /gene="rpsP"
                     /locus_tag="SCJ86_001992"
     CDS             10382..10657
                     /gene="rpsP"
                     /locus_tag="SCJ86_001992"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016175618.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S16"
                     /protein_id="HEG1169526.1"
                     /translation="MAVKIRLKRMGSKKSPFYRIVVADSRSPRDGRFIETVGTYNPLK
                     DPAEVVLKEDLVLDWLSKGAQPSDTVRNILSKEGVMQKHHEAKFSKK"
     gene            10669..10920
                     /locus_tag="SCJ86_001993"
     CDS             10669..10920
                     /locus_tag="SCJ86_001993"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337805.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="KH domain-containing protein"
                     /protein_id="HEG1169527.1"
                     /translation="MKDLSDLVLTIVRPLVTYPDQVKLDVVESKDFYEYNLTVAPEDI
                     GRIIGKQGRVAKAIRTIVYGVRINAPKKVRLNIIDDKEK"
     gene            11045..11836
                     /locus_tag="SCJ86_001994"
     CDS             11045..11836
                     /locus_tag="SCJ86_001994"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337804.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEG1169528.1"
                     /translation="MRKKMLFIPFALLLSACSSVTQESDNTHLSSSISETVTSPEKSS
                     TKTASTTEQTMATSNNEKKTALDQLKEQQPNVPMPLDVPVSSGYLNIAATHTKQGYSI
                     LYYRTDRPLGLNADELNQETPIATYLYQYGFASSQETIQVLQPFEIDTNGQQVDLGSR
                     ITGYQQGAAGSSFLEWQEGNWRIRIRGNNIEGQDPLLLAKEIVAYLEENSLPAPEQFG
                     KITVDMGDTTNRAVEVSWQEPKNAYTITHQDPMSALKMAVSMKRL"
     gene            12006..12527
                     /gene="rimM"
                     /locus_tag="SCJ86_001995"
     CDS             12006..12527
                     /gene="rimM"
                     /locus_tag="SCJ86_001995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319275.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome maturation factor RimM"
                     /protein_id="HEG1169529.1"
                     /translation="MTEYLNVGKIVNTQGIKGEVRVISTTDFPEERYKKGTVLTLFQD
                     GKAPVELTVKSHRKHKNFDLLSFEGHPSINDVEKYRDGILKVSKDNSVDLAENEFYYH
                     EIIGLHVLEDGTELGKVKEILSPGANDVWVVQRPKKADILLPYIASVVKEVDLEAGVV
                     RVEIPEGLIDDED"
     gene            12517..13278
                     /gene="trmD"
                     /locus_tag="SCJ86_001996"
     CDS             12517..13278
                     /gene="trmD"
                     /locus_tag="SCJ86_001996"
                     /EC_number="2.1.1.228"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738820.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (guanosine(37)-N1)-methyltransferase TrmD"
                     /protein_id="HEG1169530.1"
                     /translation="MKIDVLTLFPRMFEGPMGESIIGKAVDKGLLDINISNFRDYSDN
                     KHQTVDDYPYGGGAGMLLKVQPIYDNIQAIEKAAPDVKKRVILLDPAGKRFDQKMAEE
                     FSQEEHLVFICGHYEGYDERIRTLVTDEVSLGDYVLTGGELGAMVMIDATVRLLPDVL
                     GNQTSAQTDSYSTGLLEHPQYTRPAEYKGMKVPEVLTNGNHKLIEEWQLKESLRRTYQ
                     RRPDLLETLEWTPQMTKFLEEIKKEEQEKTADEAK"
     gene            13414..13761
                     /gene="rplS"
                     /locus_tag="SCJ86_001997"
     CDS             13414..13761
                     /gene="rplS"
                     /locus_tag="SCJ86_001997"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002357176.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L19"
                     /protein_id="HEG1169531.1"
                     /translation="MNPLIEELTKEQLRSDIPAFRPGDTVRVHAKVVEGTRERIQLFE
                     GVVIKRRGAGISETYTVRKVSNGVGVERTFPLHTPRVAKIEVVRYGKVRRAKLYYLRA
                     LHGKAARIKEIRR"
     gene            complement(13893..15029)
                     /locus_tag="SCJ86_001998"
     CDS             complement(13893..15029)
                     /locus_tag="SCJ86_001998"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317761.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HEG1169532.1"
                     /translation="MWIETKTDKNGKKVYKYNERYIDPKTRKRKKVSITYKNKSRETQ
                     KVALLELNKKIDIKLNEKTLQKPDLTFHELVEEWLVIYKRQVKESTYYPTNNILNTIK
                     KKIPETYIVSGINTVDLNNIFEDMIYKDDLSNKYVSVIKSKLNLLFSYAMKKGYVEKN
                     PINEVVIDYKRESKTIKIKDKFLEDDEYKRLVNFTTSHNKRYSLLFQWLYLTGMRPGE
                     AIALSKDDVHITNNNASVVINGTMMYRERSIADMKKSDSTKTAAGMREIDLPKKAIAI
                     YNELLELNPNGQFLFQTTKGTPFQLTAINTYLRNHKADMKIDKKLSSHIFRHTHISKL
                     AELGTPLYAIQDRVGHENSDITEKIYLHVTKGVKEKLKEDIEKL"
     gene            complement(15145..16059)
                     /locus_tag="SCJ86_001999"
     CDS             complement(15145..16059)
                     /locus_tag="SCJ86_001999"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319786.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4352 domain-containing protein"
                     /protein_id="HEG1169533.1"
                     /translation="MKKIVFGLLFLGLVLSGCSTSPNSASTKENTVSSTSEYETKKLT
                     SSDVHKIKVGDDLTQVYQTLGSPLRKWTNDFVYKELDNAITRDELIVSISKDAQGDYY
                     KEHSELIEHGNSAKDIKKLEMLQYTYSTDSDDETMLIWVSPKTEQVVYYNQRTFLDKD
                     GKSKDSSSTTTTTTTSSSEPQTASVAHSIGEAVPFTSENGNSINVTVQSVSKDYGDDF
                     YKPEGLFYAKVDFLVENTGTTPFDASSSYLEFYDSSDIKSDLASRDYFSETIQPGKSA
                     QGTAYFEVKNDGTTFEVFFADTSWKGSY"
     gene            complement(16150..16578)
                     /locus_tag="SCJ86_002000"
     CDS             complement(16150..16578)
                     /locus_tag="SCJ86_002000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337218.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ImmA/IrrE family metallo-endopeptidase"
                     /protein_id="HEG1169534.1"
                     /translation="MEMDTINLVEELKRKYQSANPFYICEKMGIKIQYVPFIENPKGQ
                     FQEIRGRAIIFLNDELRDSEERFYICAHELGHAIFHRGLSSYYVSTRTSRSKSESEAN
                     CFAANLIVSLYKEDNDQYPKRIEELKNLYGLPESAYRFLI"
     gene            complement(16583..16957)
                     /locus_tag="SCJ86_002001"
     CDS             complement(16583..16957)
                     /locus_tag="SCJ86_002001"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HEG1169535.1"
                     /translation="MNLLDRIKELAHKRGISITQLEEELNIPKNTIYQWKNRTPSTKR
                     LQLVADYFNVTTDYLLGRNQVPDWATKDEVVELDKLLDSNVNMSYGGETLTPEQVQRV
                     KDILIGTFWDIVKEDKEKGKKM"
BASE COUNT         5510 a         3100 c         3363 g         5166 t
ORIGIN      
        1 tttctaggaa agcgcaggaa gtattgagtt agtttgtaaa gtctgcgatc ttcaaggatc
       61 gtaaatagct gattggttgt cccatcaaca caaataaaac tcatggaacc agaaacagaa
      121 ttcattgatt tgaactcatc catacacaac accgtcggaa ggaagttcat acgtggctgg
      181 taatgcttcg ttgcttcacg caagatccgt gtcaccgtcc catccgagac gaagtacttc
      241 ttagcgatta gtttacgtga ttgattttcg tacagttcca tgagaatcac ctgcttcagt
      301 tcctttgtta gttgatgatg ttcatcaact aagtccgttt tcgcagaaaa ggtccttcca
      361 caatcatggc agagaaaacg ggtacgtttt aattctaaaa tagttaactt ttctttgact
      421 ttcaacatct gagtgcgtgt cgtataggaa ccgttgcgga taatacgaac cgatcgacag
      481 tgttcacaat aggaagaaga gtcatccaat tttcctttaa taaactgcgc ttgaacgcct
      541 ttatattctt tttcaatcag ccattcttca tcaaaaaata aatgtttatc tgttaatccg
      601 agtaattttc tagtatgatt atccattgag agactccttt ggtaggatga cgttttggtc
      661 gactaaattt taccaaaagt gagtctcttt ttgagttgaa acgaagaaaa agtgttagta
      721 gaaaatcgcc ttcgattttc caccaacaca aaatattata gaaccctttt tttttgctat
      781 atgacaggaa tgaaaggtgt ttgcaagctt aatgtgagga acactttcca tatctgttct
      841 atactggata ggagaagttt ggaaatatcc agaaaacgaa atcaatcgag gtagccacat
      901 gaagaataaa tggataaatc gaagtaaaaa aaagaaagta acacaaagat catcagataa
      961 agtcatccgt ctttttccat cgtataaaga aggattaacg gcagatcaag tcaaggaacg
     1021 aatagaaaaa ggtgcagcaa ataattcagt cgatcccaca ttcaaaacaa atcagcaaat
     1081 cgtattggaa aacatattta cgtatttcaa tttgatcttt cttatcttag ccattttgct
     1141 ttgtttagta gaatcctata agaacttgac ttttttacca gttatcatag ccaatacagg
     1201 aatcgccatt tttcaggaaa ttcgatccaa aaagatcttg gacgaactaa atgttttaaa
     1261 tacagcaaaa gtcaatgtca tacgagacgg gatagaacaa acaatagata tagaagacct
     1321 tgtaatagat gacattgtta ttttagagac tggatatcaa attccagcag atgcagaggt
     1381 cgtggaaggg aaactgcaag ttaatgaggc tttattgaca ggcgaagcag atgaaataac
     1441 caaagaaata ggtgatccat taatgtccgg cagtttcatt gtttccggaa aagcttacgc
     1501 ccgattagac aaagttggag cagattcgta tatttcccag ctgacagtca aagccaagac
     1561 aatgggagaa ggagaacagt cggagatgat tgcttctctt aatcaattga tcaaatggat
     1621 tgggatcatc attattccta tcggtatcct gcttttttct caaagttact tttttaatgg
     1681 aaacacgatc aaagaaagtg tcgttgcaat ggaagcagca ttgatcggta tgatacctga
     1741 aggactgtac ttgctgacga cgattgcatt agcattaagt gcgactaaac tagcaaagca
     1801 aagagttctt ttacacaata tgaaaagcat tgagacattg gcaagggtaa atgtcctttg
     1861 tgtagataaa acaggaacga tcacagaaaa taaaatgtct gttcaaaaaa tgatcgtctc
     1921 taaaaaacaa gaaataatca gtcagtcaga tcaattggaa gcatgcattg ctgactatgc
     1981 aaaagcgatg gcaggagaca atgcaacaat ggaagctgtt aaagcatatt ttacaaaaac
     2041 cactgatcaa tcctatcacc atgttattcc tttttcttct gtccaaaaat tcagcagtgt
     2101 agcgttggca gacaaggtgt atgttttagg agcgcccgaa atggttttgc gcgatcaatt
     2161 gccggaatat gcggaagagt ttgtttcttt cgctgaaaaa ggctatcgcg tgttggtctt
     2221 tggtgtgtac gatgggattc ttgaagaacc agtattgaca gaagcagtca caccattagg
     2281 ctatattctg attgctaatc ctatcagaaa agaagcaaga gccacatttg attacttcaa
     2341 aaaacagcaa gtagccatca aagtgatttc tggagataat ccgctcaccg tatccaatgt
     2401 agctgtccaa gcagggattt cacatgctga tcagtacgtt gatgcatcta aattatcaga
     2461 agaagaatat gaagcagctg tcgaaaaata tacagttttt ggacgcgtca agccagaaca
     2521 aaagaaaatc ttcgttcaat tgataaagcg taacaataca gtagctatga caggtgatgg
     2581 cgtgaatgat attctagcca tgaaagaagc agactgcagt atagcgatgg cttccggaaa
     2641 tgaagccgcc atgcaggcct cacaagtggt cttacttgat tctgactttt cacgcatgcc
     2701 agaagttgtt gcagaaggac ggcgcgttgt gaacaatatt gagcgatcag ccagcttgtt
     2761 tttagtgaag aatattttct cctttttgct ctcactgttt tcagtgatct ttgctctgac
     2821 ctatccttta gaaccatcac aaatcacatt gattagtttg tttactatcg gacttccatc
     2881 tttcttgctt gctctagaag aaaacaaaaa gcgaatcaga gggaaattca tcatgaatgt
     2941 gatggaaaaa gctgtgccag gtggactaac agatatgatc gtagtcggtg cgttggtgat
     3001 ctgcggggtg actttgaatc taaataaaac agatgtttct acagcatcta ccatgctttt
     3061 gattgcagta gggttcctcg ttctctataa aatctgttca ccgctgaata aattcagaag
     3121 tcagatcatt cttttctgtg ctagcggtaa ctttttttcg gtcatctttt tgcataagct
     3181 gttttcaatc accggtatct cagctgtatc acttttgctg ttgagcattc tatttttctc
     3241 ggcagattcg atttttaggc atttgaccac attggtagaa tatttgtttg aacgtaaaaa
     3301 tgaaaaagag caaaagccgt ttagtcgcgt agctaaaaaa tttctatcga tatttaaacg
     3361 agaaaattga cgaaaagtat ctcaaaaaag agtaaaaaag aacaaccact cgtgtatctt
     3421 tcgaagcagg atgagtcatc caactttaaa acgcacgagc agttgctctt ttttacattt
     3481 tcggaatggt aacggtgaaa gtcgaacctg ttccaagatg actgtctaca gtaatcgtac
     3541 cattaaggag ttgcacgtag tcttttacaa tcgctaaccc taatccggtc ccgcctgaat
     3601 gtcggcttcg cgccttgtct acgcggtaaa aacgctcaaa gatccgttcc tgatcctcaa
     3661 tgtcgatacc tataccaaag tcttggacag aaaacgatag atcatctgtt gcctgatagc
     3721 ggataataat ctcgctgtcg ctttttgaat attggacagc attttcaatt agatttttga
     3781 taattggata aaggatatct gttttagtag taaatggttc gttctttcca atcaatcgaa
     3841 tcgtcaaccg tttcttttcg atgattggct gataattttg aacgatttgc tggaaataag
     3901 gttctgtata aattgtctgg atctcgtaag aattctcgcc atcttttgac agctggatga
     3961 tttcccgaat cagtttatct aaccggatcg catctttttg catgatctca agaaaagaag
     4021 tcagagtttg cgggtcatct tttgcaccat ccagtaaagt ctcggtaaaa ccaatcagtg
     4081 aagtcacagg agtttttaat tcgtgagaga cgttgccaac gaaatccttt tgtagctttt
     4141 ctaaacgtcg cactttcgtt aagtcatacg ccacccctag aatctgtccg gtaccttctg
     4201 aattagtaaa gtagcgcaaa ctcaagtcta atgtctgccg accttctgta agggtgatct
     4261 cctgatggat cagcggatgc tcaggagtca cttgatgaat cagctgaatg agttttggct
     4321 cttgaatgac ttctgtgtac cgctgttcag gttgatagct taccacgttt aaatgtagct
     4381 gcatctttgg attaagcagt agtaaacgag aatcagcatc atcaatcagg aatacgccaa
     4441 tcatcagttc gttcagtaac gtatataatt gctcttctgt ggtagtacac gcccgataca
     4501 tcttactcat ttgttcgctc aacgcattaa tcgtctgata caattcttcc cattgatcgg
     4561 gtgtttgcat gattacttcc gcctgattag gattttgcac catttttttt agtacgggca
     4621 gcacggtttt caatggctgg tttttttgat agatcagata atagatcatt cctgcaagaa
     4681 caaggaagaa agacagaaag aagaaaaaga cccaacggcg aaaactctca gttctcggta
     4741 aaaaaccgct tgtcggttct gcgatacgta aaatggcttc taactggcca ctttttttga
     4801 ctggtaatgc gacatataaa agatcctcat tcaaagtagt acttttgcgc aaagcgctac
     4861 ctaggttccc accattcaat acggctttga tttcgggacg ggagtttcgc tgttcattca
     4921 atgtttcatt gtttgtgtca aataagatct ttccagtagc atccattaaa gtaatccgtt
     4981 cgttcgactg atggacgaat tcttccaaag cagttttgtt ttgcgctgct tggaggtttt
     5041 cgacatctaa ttgccggatc agcagttgtg ctttttttgt caggtagctt tcttgctgtt
     5101 ccacgatctg ctgttgaaaa taatgggaaa tcaattgcca gccaccaaaa aacaaactaa
     5161 ctaaaagaat aagaaagaag agacgttcat aaattttttt ccatttcatc gtttgggttc
     5221 ctgaaactta tagccaaatc ctctaatagt caccaagtac tttgggtgtt ttggatctcg
     5281 ctcgattttt tctcgcaaat gactaacgtg tacatctacg attcggcttt gtcctgcgaa
     5341 gtcaaaattc caaatgcggt ctagcaatgt gtctcggtcg atcacacggt cttttcgttt
     5401 catgaaataa acaagtaatt caaattcttt cggtgttagt tcaatcagat ggtcgtctac
     5461 ggtcacttga taattggtca aatcagcagt gatttgtcca atcgatagat attcaggctc
     5521 agcttcatct tttgattgct tacgaggttc aattcgtctg aatattgctt tcatacgggc
     5581 taatacttcg cgaggactaa atggtttggt caaatagtcg tcagcaccta tttctaggcc
     5641 aatgatccga tccacttggt catctttagc cgtcagcatc aaaatcggtg tttcattttt
     5701 ttcttgacgc agccgctgag tgatctccat gccatccatc gttggaagca tcacatctaa
     5761 aatgatgaaa tcaaatgatt gttctaatgc taattctaat ccttcgcctc catctgtggc
     5821 actggttacc ttgtagcctt ctttttccaa attgaacgtc aagagcgtta caatagaagg
     5881 ttcatcatca acaactagta tttttttcat tcaaatctcc tttaaatagg tttcattctg
     5941 tttatatttt agcatagaat cagtacaaag ggctatttta caaaagaaat aaatggtata
     6001 ataggaaggc actttgctga atagaaagga agagcagtca atgattggaa tcagtgcatg
     6061 tttaggcggt ctggcctgtc gctatgatgg acagaaaaaa gaagtaccag aattgatgga
     6121 actagttgaa actggagaag ctgtcatggt ctgtccagaa gtgatcggcg gattaccaat
     6181 tcctagagat cccgctgaaa tcattggtgg aaatggattc gatgtgtgga aagatcaagc
     6241 aaaagtatgg acgacaagcg gcgaagatgt aacggaagcg tacaaaaatg gcgcgatcaa
     6301 agcctatcaa aaattgcagg aaaaacacat tgatctagtc atcatgaagg aaaaaagccc
     6361 ttcatgcgga acccattcga tttatgacgg aagtttttcc ggaataaaaa aaaatggtgc
     6421 cggagtggct actgcttatt ttattcagca aaaaatgact gtgctctctg atgaagaatg
     6481 gctgaaaact agaggtgagc agaatggaaa tcgaaaagac caatcgaatg aacgcgctct
     6541 ttgaatttta ttcgacctta ttgacagaaa aacaaatgaa ctacatggaa ttatattatg
     6601 cagatgattt ttcattaggt gagatcgcgg aagaatacga agtaagccga caagcagtat
     6661 atgacaatat caagcggaca agcaagatct tggaggatta tgaaaaaaaa ctccatcttt
     6721 tttctaatta tattgttcga gaacaggtat tggaaaatat gactgcatat attacagata
     6781 agtacccgga agataaaaaa ctacttgaat atgtacagca aattcaagca atcgaagaat
     6841 aaataaaagg aatgaagaaa atatggcatt tgaaagctta actgaacgtc tccagcaggc
     6901 gatgggaaaa ataagaaaaa aaggaaaagt atcagaagct gacgtaaaag aaatgatgcg
     6961 ggaaatccgc cttgccttac tagaagccga tgttaacttg caagtagtca aagattttac
     7021 taagcgcgta cgcgaacgtg cggtaggggc agaagtgctg gaaagtttat cacctgcaca
     7081 acaaatcgta aaaatcgttg atgaagaatt gacgatcaca cttggttctg aaacagcaga
     7141 gttaaataaa tcaccaaaaa taccaactgt tatcatgatg gctggtcttc aaggggcagg
     7201 taaaacgacc tttactggta aattagccaa ttatttaaag aaaaatgaaa atgctcgtcc
     7261 gctcctaatc gcaggggacg tatatcgacc agctgcgatc gaccaattga aagtattagg
     7321 tcaacagcta gacgttcctg tatttgatat gggaactgaa gtcagcccgg tagagatcgt
     7381 ccgtcaaggg atggagttag caaaagaaaa gaaaaacgac tatgtattga tcgatacggc
     7441 aggacgtctg catatcgatg aaacattgat ggatgagttg aaacaaatca aagaattgac
     7501 gcaaccaaat gagattttgc ttgttgtcga tgccatgact ggtcaagatg ccgtcaacgt
     7561 agctgatagc ttcaaccagc aattaggtat tacaggggtc gtcatcacta aacttgatgg
     7621 ggatactcga ggcggggcag cactatcgat ccgctcagtg acaggcgcac caatcaaatt
     7681 catcggttct ggtgaaaaat tgactgactt agaagtattc cacccagatc gtatggctag
     7741 ccgtatcctt ggtatggggg atatgctgac attgattgaa aaagcgcagc aagactacga
     7801 cgagaaaaaa gcggaagaac ttgcacagaa aatgcgggaa aattcattcg atttcaatga
     7861 ctttattgaa cagcttgatc aagtgatggg tatgggccca ctggaagatc taatcaaaat
     7921 gatcccagga atgaatcaag tccctggaat cgaaaacgtc aaggtagatc caaaagatgt
     7981 tgaacgcaaa aaagcgatgg tttattctat gacgccagca gaaagagcaa atcctgatct
     8041 gttgaaccca agccgtcgtc gccgaattgc tgccggttct ggaaacagtg ttgttgaagt
     8101 gaatcgaatg atcaaacaat tcaaggaatc aagaaaaatg atgcagcaaa tgtccaaagg
     8161 ggatatgaac atcccaggta tggaccaaat gtttggttca ggcgtcaaag gaaaactagg
     8221 caaaatggcg atgaatcgaa tgatcaagaa aaataagaag aaaaagaaaa aacggaaata
     8281 agatgaaaaa aacagcttcg gtacagactg aaaatgtcta ctgccggagc ttttctatca
     8341 gcaattttta ttctttacta atacaattac tctaaacgaa gaggtggcac agatgagatg
     8401 tccgtggagt gaacaaacag aaagtatgcg gcaatatcat gatactgtct ggggtgttcc
     8461 tgaatatgat gaccaaaagc tgttccgaaa attgatgctt gatattaatc aagccggatt
     8521 gagctggcag acgattttaa ataaaagtga agcattcgat gaagcatatg atcgattcga
     8581 tatcaaaaaa gtcgcaaatt atggagaaac aaaaatcagt gaactaatgg aaaacaaagg
     8641 aattattcgt aaccgaagaa aaatcgaagc agccgtccaa aacgccaaag cagtcttgaa
     8701 aattcaagaa gaaatcggca gtttttcaga gtatctttgg tcgttttcta atggaaaaat
     8761 cgttgattct tcttatcatt cacaagaaga agtcccaaca acaagtcctc tttcagatga
     8821 attagcaaaa gatctaaaga aaagaggatt taaatttatc ggaagcacaa caatctatgc
     8881 ttttttagaa gctgtcggga tcatcaatga tcaccttgac agttgtttcc gcaaagaaga
     8941 ggtcgtacgt atcggcagaa agggcgaata aagcatgcga aaaatcatat tttatggtgc
     9001 tataagttta gatggctatt tggcaacgaa aacggatgat cttcaatggc tttttgacac
     9061 tccaacaggt aagcaaacta cttatgaagc tttttatgaa acaatcgata cgaccatcat
     9121 gggcagaaaa acttatcagg aagcaaaaaa atatttggag accgaagcaa tttatccaga
     9181 gaaaaagaat ttcgtttttt ccacccacgc acaattacaa ttgccagatg ctgaggtcgt
     9241 aaacgaagat cctgttgctt ttctaaaaaa tctacaaatg acagaaggaa aagatatctg
     9301 ggttgtgggt ggcggtcgat tgatcaaacc gctattggaa catcaaatga ttgatgaatg
     9361 gtatatccag attacccctg tattattagg ggacgggatt cctttattcc aaaaaggaac
     9421 atatgaagcg cgttttgaaa tgctagacac gacacgattc ggtgaattta tcgaattgca
     9481 ttatgtacga aagtagaagt aaataaaata aaaaaggcaa ctatatgcag aaatgtcctg
     9541 tacatagttg ccttttttat taccgttatg ctttttactc tgttaatttg ttagaaaaat
     9601 ctagatacgc atctaaaatc gtttggaaga aacttaatgt tccttcaacg atattaccat
     9661 tttcatcaat tagattcgca acgtttccga tatatgcttc cggttgctgc aaggttggca
     9721 cattcaaaaa gacaagtgat tggcgtagat ggtggtttgc gccaaagccg ccgatcccac
     9781 ctggtgatgc agtaacgact aaacctggtt tatgatccca aacactgtgt ccataaggtc
     9841 ttgagcctac atctaaagca tttttcaata ctgctggcac agaacggttg tattcaggag
     9901 taacgaaaac gacaccgtct agtcccctca cttcctcacg aaaacgagtc cattcagcag
     9961 gagcctgttc tggagtttcg agatcctcat tgtaaaatgg cagatcatcg atttttacaa
    10021 atacactttc gtacccttca ggaagcaatt tcgcaaaagc ttctgctact tttttgttgt
    10081 aagaatcttt tcttaaacta ccaataaaaa atccaatttt ttttgtcata cgatttttcc
    10141 tccttcaaac tatctataat aatcttacaa aagataagta actggagcaa ataaaaacac
    10201 ttactgacaa aagctacata gaagatagat tacttatttt taacaaaaaa gaagtctaaa
    10261 atcttctatt ttctaaaaaa aatcgaattt aatcaagctg taaagaaaaa aagctttaca
    10321 gggtaaaaaa aaactgttac actacatctt gtgaataaaa caatggaggt ggaaataaat
    10381 aatggcagtt aaaatccgtt taaaacgtat gggttctaaa aagagtccgt tttaccgtat
    10441 cgtcgtagct gattcacgtt ctcctcgtga tggacgtttc atcgaaactg taggtactta
    10501 caatcctttg aaagaccctg cagaagtagt tttaaaagaa gacttagttc ttgactggtt
    10561 atcaaaaggt gcacaacctt ctgacactgt acgtaacatc ctttcaaaag aaggcgttat
    10621 gcaaaaacac cacgaagcta aattctcaaa gaaataaggt gaccggatat gaaagattta
    10681 agcgacttag tcttaacaat cgttcgtccg ctagtcactt atcctgatca agtcaagtta
    10741 gacgtcgtag agtcgaaaga cttttacgag tacaatctaa cagtggctcc tgaagatatt
    10801 ggccgtatca ttggaaaaca agggcgtgtt gctaaagcaa tccgcacaat cgtttacggt
    10861 gttcggatca atgcaccgaa aaaagtgcgt ttgaatatca tcgatgataa ggaaaagtaa
    10921 ataaacgtgc tgtactcgaa ttttcgagtg cagctttttt gtttttttta ttaattgaag
    10981 aaatgattat ttggcaaaac aagcaagaaa gcgtatcctt ataggaggag ggagtgaaca
    11041 gccaatgaga aagaaaatgt tatttattcc atttgctcta ttgttaagcg cttgtagttc
    11101 ggttactcaa gaatcagaca atacacatct atcaagcagt atatctgaaa ctgttaccag
    11161 tccagaaaaa agtagtacca aaacggcttc caccactgaa caaacaatgg ctacctcaaa
    11221 taatgagaaa aagactgctt tagaccagtt gaaagaacaa cagccaaatg ttcccatgcc
    11281 tctagatgta ccagtctctt caggctatct caatatcgca gcgacacata caaagcaagg
    11341 ttattctatt ttatattaca gaacagatcg tcccctcggg ttgaatgctg acgaattgaa
    11401 tcaagaaacg cctattgcaa cctatctata tcaatatggg tttgcttcta gtcaagaaac
    11461 aatccaagtt ttgcagccat ttgagatcga cacaaatggt cagcaagtgg acttaggttc
    11521 tcgaatcaca gggtatcagc aaggtgcagc aggttcaagt tttcttgaat ggcaagaagg
    11581 aaactggcgt attcgaatcc gtgggaataa tatagaaggc caagatccct tactattagc
    11641 taaagaaatc gtagcttatt tggaagaaaa cagtctacca gcacctgaac aatttggtaa
    11701 aattactgtt gatatgggtg atacgacgaa tcgagccgta gaagtaagct ggcaagaacc
    11761 gaaaaatgcc tatacgatca ctcatcaaga tcctatgtct gcgttgaaga tggcagtttc
    11821 tatgaagagg ttgtgaaatc agcgcgctga tctgagccca tcatttaact ccaagtcaca
    11881 ggaagtaatc aaaaatagca cgccgcattt ttgcataatt ttggcttaat accttaaaag
    11941 aaaactagaa ttgcctcttc acacatgcta aaataaaaca gacaaaaaag aaatggggat
    12001 ataagttgac tgaatacttg aatgtaggaa aaatcgtaaa cacacaaggt atcaaaggcg
    12061 aagtccgtgt tatctcaaca acggatttcc cagaagaacg ttacaaaaaa ggaacggtgc
    12121 taacactatt ccaagacgga aaagcacctg tggaattaac agtcaaaagt catcgcaaac
    12181 ataaaaattt tgacttattg agttttgaag gacatccttc tatcaatgat gtggaaaaat
    12241 atcgggatgg catattaaaa gtgtcaaaag acaactctgt agatctagct gaaaatgaat
    12301 tctattacca tgagatcatc ggtctgcatg tattagaaga tggaactgag ttaggtaaag
    12361 taaaagaaat cttgtcacca ggtgccaatg atgtttgggt cgttcaacgt ccgaaaaaag
    12421 ctgatatatt gcttccatat atcgcttctg tcgtcaaaga agtcgattta gaagctggag
    12481 tggtccgtgt agaaattcca gaaggactga ttgacgatga agattgatgt attgacttta
    12541 tttccaagaa tgtttgaagg tcctatgggg gaatccatca tcggtaaggc tgtagataaa
    12601 ggattattag atatcaatat ctctaacttc cgcgattatt cagataacaa acatcagaca
    12661 gtagatgatt atccatacgg cggtggagca ggtatgctgc tcaaagtcca gcccatctat
    12721 gataacatcc aagccattga aaaagctgca ccggatgtca aaaaaagagt aattcttctt
    12781 gacccagctg gtaaacgatt cgatcagaaa atggcagaag aattttcaca agaagaacac
    12841 ttagtattca tttgtggtca ttatgaggga tatgatgaac gtatccgcac attagtgaca
    12901 gatgaagttt ccttaggaga ttatgtcctt actgggggag aattaggtgc gatggtcatg
    12961 atcgatgcaa cagttcggct tttgccagat gtgttaggga atcagacttc agctcaaacg
    13021 gattcttatt caaccggttt attggaacat ccccaatata ctcgcccggc agaatataaa
    13081 gggatgaaag taccagaagt attgacaaat ggaaatcata aattgattga agaatggcaa
    13141 ttaaaagaat cactaagaag gacctatcaa cgtcggcctg atcttttgga aacacttgaa
    13201 tggacacctc aaatgacgaa atttttagaa gaaatcaaga aagaagaaca agaaaaaaca
    13261 gcagatgaag ccaagtgact ttttctaaaa tcttctttac agtaggttga taatatgtta
    13321 gtattttatg gtgagtgcaa aagcacttaa ctattacgat attccgctgt gagaaaagct
    13381 cataagaata tttggaataa ggagaagaaa accatgaatc cattaatcga agaattaaca
    13441 aaagaacaat tacgttctga catcccagcg ttccgccctg gtgacactgt gcgtgttcac
    13501 gcaaaagttg ttgaaggtac tcgtgaacgt atccagttat ttgaaggtgt tgttatcaaa
    13561 cgccgcggtg ctggaatcag cgaaacttat acagtacgta aagtttctaa cggagtgggc
    13621 gttgaacgta cattcccatt gcatacacca cgtgttgcaa aaatcgaagt agttcgctac
    13681 ggtaaagtac gtcgtgcaaa attgtactac ctacgtgcat tacacggaaa agctgctcgt
    13741 atcaaagaaa tccgtcgtta atcatcgtta tcagactgag aaagaaccct tggtattcaa
    13801 gggttctttt tttgttctct gtttttttat attaacgatt tctatttggg acatttttga
    13861 ttaataaaaa ggggcaaaaa aggggcaaca agttacagtt tttctatatc ttctttcaat
    13921 ttttctttta ctccctttgt cacatgcaaa taaatctttt cagtgatatc actattttcg
    13981 tgcccaacgc gatcctgaat agcatacaga ggagttccta gttccgctaa ttttgaaata
    14041 tgggtatgtc taaaaatatg tgaactaagt tttttatcaa tcttcatgtc agctttatga
    14101 tttctcaaat aggtgttaat tgctgttagt tggaaaggag ttcctttcgt tgtttgaaat
    14161 agaaattgac cattcggatt taattctaga agctcattgt aaatagctat cgcttttttt
    14221 ggtaaatcaa tttctcgcat tccagcagca gtttttgtag aatcagattt tttcatatca
    14281 gctattgaac gttctctata catcatcgtc ccatttataa ctactgatgc attattattg
    14341 gtaatgtgta catcgtcttt acttaatgcg attgcttctc caggcctcat tccagtcaaa
    14401 tatagccact ggaaaaggag agaatatctt ttattgtgtg aagttgtgaa atttactagt
    14461 cttttatatt catcatcttc taaaaactta tctttaattt ttatcgtttt tgactctcgt
    14521 ttataatcaa taactacttc gttgatagga tttttttcta catagccttt tttcatagcg
    14581 tatgaaaaaa gaagattcaa tttggattta attacactga cgtacttatt tgacaagtca
    14641 tctttatata tcatatcttc aaaaatatta tttaaatcta ctgtattaat accagaaacg
    14701 atgtaagtct caggtatctt tttctttata gtgtttaaga tattattcgt aggataatat
    14761 gtagactcct taacttgtct tttgtaaata actaaccatt cttcgacaag ctcatgaaat
    14821 gttagatcag gcttctgaag tgttttttcg tttaatttta tatcaatttt tttatttaac
    14881 tctagcaatg ccactttctg agtttctcga gatttatttt tataagtaat gctcactttt
    14941 tttctttttc tagtttttgg atcaatatat cgctcattat atttatatac tttttttccg
    15001 tttttatcag ttttggtttc aatccacatt gttttcatct cctatttgtt gctataatag
    15061 gaatggataa gtaagccaat tatagcaggt ttatttttca tcacgtccac aaactttggc
    15121 gagggagggg gcgtgttttt ttgtttagta gctacctttc catgaagtat ctgcgaagaa
    15181 cacttcaaat gttgttccat catttttcac ttcaaaatat gctgttcctt gtgcggattt
    15241 acccggttgg atggtttccg aaaagtaatc tctactagct aaatcagatt taatatctga
    15301 tgaatcataa aattctaaat aacttgaact tgcatcaaaa ggtgtagtcc ctgtgttttc
    15361 tacaagaaaa tctacctttg cgtaaaatag gccttctggt ttatagaaat catcaccgta
    15421 atcttttgat acagactgga cagttacgtt aatagagtta ccattttcag aagtaaaagg
    15481 aactgcttct cctatactat gtgcaactga tgccgtctgg ggttctgaag acgaagttgt
    15541 agttgtagtt gtagtagagc ttgaatcttt ggattttcca tctttatcta agaaagttcg
    15601 ttgattataa tatacaacct gttcagtttt tggactaacc caaattaaca ttgtttcatc
    15661 atcagaatca gttgaataag tatattggag catctctaat ttttttatat cttttgcact
    15721 atttccatgt tcaattaatt cagagtgctc tttataataa tctccttgtg catcctttga
    15781 aatagataca attaattcat cccgtgtgat agcattgtct aattctttgt aaacaaaatc
    15841 atttgtccat ttacgtaacg gtgaaccaag tgtctgataa acttgagtta aatcatcacc
    15901 aacttttatt ttatgaacgt ctgatgaagt tagttttttt gtttcatatt cagatgttga
    15961 tgaaacagta ttttctttag tgctagcaga attaggacta gtggagcatc cgcttaatac
    16021 caagcctaaa aaaagcaacc caaaaacaat ttttttcatt tttaattcct cacttctatg
    16081 atatgatttt tttgtaggat cttagaaata aggtttttag tccgtgttgc agcacggact
    16141 ttttttacat tatataagaa agcgataagc gctttctgga agtccgtaaa gattctttaa
    16201 ttcctcaatt cgtttaggat attgatcatt gtcttctttg tatagagaaa caatgagatt
    16261 agcagcaaag caattagctt cgctttcaga tttgcttcta gatgttcttg ttgatacata
    16321 gtaactggat aagccacgat gaaaaatagc gtgacctaat tcgtgagcgc aaatgtagaa
    16381 tctttcctca gagtctctca gttcatcatt taagaagatt attgcacgac ctctaatttc
    16441 ttgaaactgt cctttgggat tctcgataaa aggaacgtat tgaattttaa tgcccatctt
    16501 ttcacaaata taaaaaggat tagcggactg gtatttccgc ttcaactcct cgactaaatt
    16561 aatcgtatcc atctccataa gctcacatct ttttgccttt ttctttgtct tctttcacaa
    16621 tatcccagaa ggttccaata aggatatctt ttacgcgttg gacctgttca ggcgtcagtg
    16681 tttctccgcc ataagacata ttgacatttg aatctagtaa tttatcaagt tcaactactt
    16741 catcttttgt tgcccagtca ggaacttggt ttctaccgag taaataatca gtagttacat
    16801 taaaataatc tgctacaagt tgtagtcttt tagtactagg agttctattt ttccattgat
    16861 aaatcgtgtt tttaggtata tttaattctt cttccaattg agttatactt atgcctcttt
    16921 tatgagctaa ttcctttatt ctatctagta aattcatttt cgcacctcac aagctacgaa
    16981 aacactaata aaaaaattag ttaaaggtgt tgacaactaa taaaagtgtt agtatactgt
    17041 tttcgtaagc taaaatatta gctaaaaaga cttgataact aataaacact tcacggtcgg
    17101 caaactctga aatgtaaatt actaggcatt tctgtgtct
//
LOCUS       DARBET010000055        16668 bp    DNA     linear   BCT 07-NOV-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4027
            SAMEA114569841-rid20112183.denovo.057, whole genome shotgun
            sequence.
ACCESSION   DARBET010000055 DARBET010000000
VERSION     DARBET010000055.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA114569841
            Sequence Read Archive: ERR12203666
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 16668)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16668)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001972618.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001972618.1
            Genome Coverage       :: 132x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/07/2023 13:18:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,041
            CDSs (total)                      :: 2,975
            Genes (coding)                    :: 2,908
            CDSs (with protein)               :: 2,908
            Genes (RNA)                       :: 66
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 67
            CDSs (without protein)            :: 67
            Pseudo Genes (ambiguous residues) :: 0 of 67
            Pseudo Genes (frameshifted)       :: 26 of 67
            Pseudo Genes (incomplete)         :: 28 of 67
            Pseudo Genes (internal stop)      :: 20 of 67
            Pseudo Genes (multiple problems)  :: 6 of 67
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16668
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA114569841-rid20112183.denovo.057"
                     /isolate="VRE4027"
                     /db_xref="taxon:1352"
                     /country="Denmark"
     gene            complement(157..864)
                     /locus_tag="SCJ86_002002"
     CDS             complement(157..864)
                     /locus_tag="SCJ86_002002"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853426.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc metallopeptidase"
                     /protein_id="HEG1169536.1"
                     /translation="MYGYGMYGFLDPTFLLVILGLVISGIASAYVNSTFRKYDQVRSK
                     NNVTGTQAAQYILQSQQINNVGVQQIAGDLTDNYNSGNKMLSLSQATAQSTSVAAIGV
                     AAHECGHAVQDASGYVPLKIRAALVPAANFGSMISFPLIMVGVLLSWNSTLINIGIFA
                     FSLALLFQLVTLPVEFNASRRAIQILSEGGLLTEEEVPMARHVLFAAALTYVAAALST
                     FLQLLRLILLFGGNRRD"
     gene            complement(943..1281)
                     /locus_tag="SCJ86_002003"
     CDS             complement(943..1281)
                     /locus_tag="SCJ86_002003"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315786.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alkylphosphonate utilization protein"
                     /protein_id="HEG1169537.1"
                     /translation="MTLPNCPECGSEYTYEDRGLLICPECGNEWNKEEQETVIEDGLV
                     VLDANGNRLLEGDSVTVIKDLKVKGASGAIKQGTKVKNIRLVEGDHNIDCKVEGFGPM
                     KLKSEFVKKN"
     gene            complement(1392..2354)
                     /locus_tag="SCJ86_002004"
     CDS             complement(1392..2354)
                     /locus_tag="SCJ86_002004"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional oligoribonuclease/PAP phosphatase
                     NrnA"
                     /protein_id="HEG1169538.1"
                     /translation="MTVQQEIVDTIKKYDKIIIHRHLRPDPDALGSQVGLAELLRESF
                     PEKEIRQVGETIEGLRFLAEMQEVEDVFYQGALVIVTDTANSPRISDQRYQLAEKLIK
                     IDHHPNDDPYGDLVWVNTKASSCSEMIAEFALMFPESLKMNVKAARLLYAGIVGDTGR
                     FLYPSTTSRTLEIASILRNYPFDASALNREIEQMPMKVAKLSGYLYQNIQVDENGAGR
                     IILPQEILDKYGIDDSETASIVSLPGVIDEVLAWGIFVQQPSGYYRVRLRSKGPIINE
                     LAKRHHGGGHPLASGANAKDLAEVEEIYKEIQDICQSYKSRKTE"
     gene            complement(2369..3700)
                     /locus_tag="SCJ86_002005"
     CDS             complement(2369..3700)
                     /locus_tag="SCJ86_002005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341209.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CBS domain-containing protein"
                     /protein_id="HEG1169539.1"
                     /translation="MATKHDLILEYIESLPVGNRISVRSIAKELNVSEGTAYRAIKDA
                     ENVGLVSTIQRVGTIRIERKLKKHIERLTFGEVVRIIEGDVLGGAAGLDKVLNKFVIG
                     AMTKHAMERYITPGSLMIVGNRAEVQKLALEDGAAVLITGGFNTTAEIAKLADDLEMP
                     ILRTSYDTFTVATMINRALSDQLIKKDIMLVSDIYMPAEKTHYLQQTDTISDYQRLSE
                     ETQHSRFPVVNRHHRLMGIVTAKDVLGKSPNQIIDRVMTKEPISVKKTMSIASVSHQM
                     IWDGLEVMPVVSDDLTLEGLITRQDVMKAMQLVQRQPQIADTISDQISGNIHTIDTDR
                     EGNRLENPKFKFVVAPQMVNGVGTISFGVLSEIISDVAQKTMVMNQKRNILIEQVNLH
                     YLRLIQLESELDIRPRVLEIGRRSAKLDIEVFIENTIVSKAIVVCQVMERS"
     gene            complement(3708..4388)
                     /locus_tag="SCJ86_002006"
     CDS             complement(3708..4388)
                     /locus_tag="SCJ86_002006"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal-dependent hydrolase"
                     /protein_id="HEG1169540.1"
                     /translation="MKITYHGHSCLTIEMENGQRVMIDPFITGNPLTDLTAEEVKTDW
                     LLITHGHSDHIGDMIPIAKENQATIISIVEIADYANSRGVDSFGMNIGGKHAFPFGVV
                     KFVHAQHSSSYEVDGIVQYMGEPSGIIIQAEGKTIYHAGDTAYFSDLGLLAEEFDIDV
                     AFLPIGDNYTMGPEDAAKAAQTIRAKKVVPIHYNTFPVIEQDPESFVELLEDSEGKIM
                     SVSETLEV"
     gene            complement(4607..5086)
                     /locus_tag="SCJ86_002007"
     CDS             complement(4607..5086)
                     /locus_tag="SCJ86_002007"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminoacyl-tRNA deacylase"
                     /protein_id="HEG1169541.1"
                     /translation="MMSQTKVERYLEEQKISYQPLDFSHVVEESFTEELQKRGIDPAL
                     ICKTLVVKGNKTGVIIALIPLNDHLDYKKTRQVTKDRKVGFPGMDFVLMHTGYPHGAN
                     TPIGIKLAHPDYCFLADSSLKEKEEVIVSSGEIGRSLRIKVADLEKIVHPIYADLIS"
     gene            complement(5213..6892)
                     /locus_tag="SCJ86_002008"
     CDS             complement(5213..6892)
                     /locus_tag="SCJ86_002008"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298952.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease J"
                     /protein_id="HEG1169542.1"
                     /translation="MSTIKIIPLGGVRENGKNMYVAEVGEDIFILDCGLKYPENELLG
                     IDVVIPDFTYLEENIDRVAGVFLTHGHADAIGALPYLLSKISVPVFGTELTIELAKLN
                     VSRNEAAKGFKDFHVVDEHTEIDFGETVVSFFRTTHTIPDSVGINLKTKEGSIVYTGD
                     FKFDQTAIPMYQTDYARLAEIGKEGVLALLSESANAENPQPVASEAQIADEVFDTIKY
                     WEGRIIVACVASNLQRVQQVLDAAEKAQRKVVLTGQDFERIIRTAMRLEKLQLPSEDL
                     LVTLKDMKNYDPEELIILETGRMGEPIKSLQKMANGTHRNVKIEEGDLVYITTTPSIA
                     METIVAKTEDIIYRAGGTVKLISENMRVSGHANPNDLQLMINLMKPTYFVPVQGEYRE
                     LAAHADLAHAVGMPYKNIYITGRGDVLEYKNKRMSVAGTTSAENVMIDGLGIGDIGNI
                     VLRDRKILSEDGIFVAVVTISRREKKIISKPQITSRGFVYVKASRDLMKESSNMIETI
                     VEKHLESNDFEWSKLKQDIRDQLGRYLFEQTKRRPVILPVIMEATQRRNKK"
     gene            7440..8942
                     /locus_tag="SCJ86_002009"
     CDS             7440..8942
                     /locus_tag="SCJ86_002009"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338688.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEG1169543.1"
                     /translation="MNFMKRAWKSTKVKWGRSLLLFAVFTAILIFVLAGLTIRSAAEE
                     AANQAQKEVGATVTLSANREASFKKQEQTSSSGSTTEERPDPGSFSLTPVSLTDAEKI
                     AELDNVKSYSFESSASAMAKDGITAITSSDSSEDSSETTDAGGEPAGGMGGNPQMTQA
                     DFQISGVSATAQNSNFSDGTAKITDGEGIDDSDKGTNNVVIDSTLAETNDLAVGDTFT
                     ITSTEDEDTTYEMTIKGIYESSETSGSMGMKFNFMNPSNTLYTSYTFANELNGTSDDN
                     TIDSAIYTLSDPDKMDEFLKEAEKLIDTDTFSLQSNDSMYQSMLEPLNNVASFSKNVV
                     LLVAVAGIIILTLIIMITIRERRHELGVLLSLGESRSKIILQLFTEVAICMILALGVA
                     SLSGNVVANAVGQQLLDQQTETTTQNQQAPGGGQMPGNRQEQGGQRDGQQGTTNNPFA
                     VSDQVSELEINVKPAQLGLLAGVAFGISLFSVFLASIGILRLNPRKILLN"
     gene            8955..9617
                     /locus_tag="SCJ86_002010"
     CDS             8955..9617
                     /locus_tag="SCJ86_002010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298949.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEG1169544.1"
                     /translation="MLRTEQLGFWYQDPQDALYKDVELNFEAGKMYAILGASGSGKTT
                     FLSLISGLDAPKEGKVYYQDRSLNKIGLRSYRKNDVSIIFQAYNLLPYLSALDNVIAA
                     MEITKTKQENYRQVALDRLARVGIDEILAKKQVTKLSGGQQQRVAIVRAICCEHQLIV
                     ADEPTGNLDETTSQDIVKLFQEIAHEQNRCIIIVTHEIEVAKSCDEVFELKNKKFQKI
                     EV"
     gene            complement(9718..9921)
                     /locus_tag="SCJ86_002011"
     CDS             complement(9718..9921)
                     /locus_tag="SCJ86_002011"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CsbD family protein"
                     /protein_id="HEG1169545.1"
                     /translation="MADLKGRSKDMKDKVVGEGKEAYGKATDDKSKETEGKAQSLGAD
                     IKEKARNLGDEVKDKFSRKDEKK"
     gene            complement(10032..11216)
                     /gene="iadA"
                     /locus_tag="SCJ86_002012"
     CDS             complement(10032..11216)
                     /gene="iadA"
                     /locus_tag="SCJ86_002012"
                     /EC_number="3.4.19.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014387121.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-aspartyl-peptidase"
                     /protein_id="HEG1169546.1"
                     /translation="MKIIRQIEVFAPDYLGKMDVLIAGGKILAVEEQLEGGYEGVEVE
                     ELSGEGKVLTPGFIDCHFHILGGGGEGGYQNRTPEVTLSQLTTAGVTTVVGCLGTDGE
                     GRDMTALISKAKGLEAEGISTYVYEGSYRLPVKTVTDSIIKDFLTIDKIIGIGEIAVS
                     DHRSSQPSFEEFARAVADARVGGMLSGKAGIINVHLGGGKRKLELLTRIVEETEIPIT
                     QFLPTHANRTPELFEACVAFAKRGGTIDFTASEDPDFWEKTDGEVRFSKALKRLIEED
                     VSLDNFTMTSDGQGSLPYFDENNHFLGLGVGSAKALLVGIKEAVQKESIPLEIALRAI
                     TSNPARILKLDKKGKIEIGADADLCILDKETLDIDTVIAKGEIMVQEKEVKVWGTFEK
                     SF"
     gene            complement(11373..13112)
                     /gene="yfcC"
                     /locus_tag="SCJ86_002013"
     CDS             complement(11373..13112)
                     /gene="yfcC"
                     /locus_tag="SCJ86_002013"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301881.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative basic amino acid antiporter YfcC"
                     /protein_id="HEG1169547.1"
                     /translation="MKKTNSSGKPKRDFTKLSTPHTYVIIFGVVIFAWILTFVVPAGK
                     FSTQDIEYKDANGETSTRTVLRQDSFRYAYELDKSYVFDQLEELQDHPAEREKLDVPE
                     KGLEKVIADGEKNLTQEKLDEISLTDDVLYDQYGENIYDTSKKLHKTAKIWGTDDFGG
                     FGFLNFVFEGLVSGDKYGSAVGIAALILVVGGAFGIIMRTGAIDAGIYAFISKTKGLE
                     RLALPLLFFAFSFGGATFGMAEEVIPFSMVMVPFVIALGYDSIVAVTVTYVASQVGNA
                     TSWMSPFSVAVAQGIAGIPVLSGATFRLIMWVVVTALAAGYMMIYAEKIRKKPESSLT
                     YKSDDYFRSHIKKTSDENKKFMLGHKLILLEMLVVLVWIVWGVTQKGYYIPEIASQFF
                     VMGLVAGIIAVLFKLDGMKVNDIASSFQSGAADLAGTAIVVGMAKGILLVLGGSDASV
                     PSALNTILHGIGTALTGVPAVIGAWAMYIFQSLFNLVVTSNSGQAALTMPIMAPLADL
                     VGVSRQVAVLAYQLGSGFMDAFTPVSASLIGVLGVARIEWAKWARFQIKMQGFLFVLG
                     TIFIMIAIAIGLQ"
     gene            complement(13885..14499)
                     /locus_tag="SCJ86_002014"
     CDS             complement(13885..14499)
                     /locus_tag="SCJ86_002014"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339759.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SIS domain-containing protein"
                     /protein_id="HEG1169548.1"
                     /translation="MSLCYFDYYHQLEEKLRIEEPLMLQAGTWIAKQINQGGRLQIYA
                     SRTLSGVAFEFWDQTPDLVPSILIENPAGGVYETLEGAGQAIIDQLSVKSEDIFLLLS
                     NEGRNPAIIELAQWIKENGHPLIIVTGFDLSRSIKSQHSSGLRLFEFADLVLDNHANL
                     HDAVLTLPNLDPAICGTASLATIFLLQQVLYFTAKQLVQDTSGK"
     gene            complement(15105..15482)
                     /locus_tag="SCJ86_002015"
     CDS             complement(15105..15482)
                     /locus_tag="SCJ86_002015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEG1169549.1"
                     /translation="MGKLVAVAQSLLDILKWLMEGSNWIFVLPLLLISLFIMFRMVRK
                     KETFLIFIWYAASVGVATLMVELALGSWEACVFALFFAVLYLIIYIPYMKRKSRIGRK
                     GRHTTTFSSTDSLEQSRRTRRRR"
     gene            complement(15782..16663)
                     /gene="galU"
                     /locus_tag="SCJ86_002016"
     CDS             complement(15782..16663)
                     /gene="galU"
                     /locus_tag="SCJ86_002016"
                     /EC_number="2.7.7.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UTP--glucose-1-phosphate uridylyltransferase
                     GalU"
                     /protein_id="HEG1169550.1"
                     /translation="MKVKKAVIPAAGLGTRFLPATKAMAKEMLPIVDKPTIQFIVEEA
                     LDSGIEDILIVTGKEKRPIEDHFDANIELENNLREKEKTELLALVEETTQVNLHFIRQ
                     SHPRGLGDAVLQAKAFIGNEPFVVMLGDDLMKDDIPLTQQLMMDYEETQASAVAVMRV
                     PENETSKYGIIDPKTDSEKGRCRVKGFVEKPEMGKAPSNLAIIGRYLLTPKIFEILET
                     QEPGAGNEIQLTDALQTLNQTEAVYAREFKGKRYDVGDKLGYMKTNIEYGLQHSEIGT
                     SLSAYIIQLSKKLRTES"
BASE COUNT         4829 a         3593 c         2847 g         5399 t
ORIGIN      
        1 ctgagccaga atgaggttca ttctgaccca gatttgattt tgtacaaccc atcagtcctt
       61 attgacaaag agcccttatt ccaaataaat ggtattcaat cagctaactc tcggtattag
      121 ctcagatcaa agcgctgatt tcacaacttc tttattttaa tctcttcggt tgccgccgaa
      181 caataagatc aagcgcaaca gctgcaggaa tgtcgaaagc gctgctgcaa cataagtcag
      241 tgctgctgca aataatacgt gtcttgccat cggtacttct tcttccgtca gtagtccgcc
      301 ttcactcaaa atttggattg ctcgccgaga cgcgttaaat tcgactggta atgtcaccaa
      361 ttggaaaagt aatgctaaag aaaaagcgaa aatcccgata ttgattaacg tagagttcca
      421 gctaagcaat acacctacca taatcaaggg gaacgaaatc atcgaaccaa agttagctgc
      481 tggaacaagt gctgcacgaa tcttcaatgg gacataaccc gaagcatctt gtaccgcatg
      541 tccgcattca tgagctgcta cacctatcgc agcaactgaa gtagactgtg cagttgcttg
      601 agacaaactc aacatcttat tacctgaatt gtagttatct gtgagatctc cggcaatttg
      661 ttgaacaccg acattattta tctgttgact ttgtaatata tattgtgctg cttgtgtacc
      721 agtcacattg tttttgcttc tcacttgatc gtattttcgg aaagtactat tgacgtacgc
      781 tgacgcaatt ccggaaataa ctaatccaag aatgaccaaa agaaatgtcg gatctaaaaa
      841 accatacatt ccataaccat acattttcgt ttcactcctt ttcgtttact ttattttatc
      901 attagtagta ggaaaagaat gtgaaaagac cttgaatatt ctttaattct ttttcacaaa
      961 ttccgatttc agtttcatcg gtccaaatcc ttctaccttg caatcgatat tatgatcgcc
     1021 ttctactaaa cggatgttct tgacttttgt tccttgttta atggcacctg acgcaccttt
     1081 tactttcaag tctttaatca ctgtcacgct atcgccctct aacagacggt ttccatttgc
     1141 atctaatacc actaatccgt cttctatgac agtttcttgt tcttctttgt tccattcgtt
     1201 cccgcattct gggcaaatca acaagcctcg atcttcatat gtgtactctg agccacattc
     1261 tgggcaattt ggtaatgtca tcttctttct cctttaaaat ctttagtgag cactattgta
     1321 acggaaaaag aagatcttga aaagatacat ttgtgatttt tactacaaaa ggcacgtttt
     1381 cgaacgaagt atcattctgt ttttctgctc ttatacgatt ggcatatatc ttggatctct
     1441 ttatagattt cctctacttc tgctaaatct tttgcattcg caccacttgc taatggatgg
     1501 cctcctccat gatggcgttt agccaattca ttgatgatcg gccccttaga acgtaaacgt
     1561 acgcggtaat accctgaagg ctgctgaaca aaaatccccc aagccagcac ttcgtcaatc
     1621 actccaggaa gtgaaacgat cgaagctgtc tctgaatcat cgattccata tttatcaaga
     1681 atttcttgcg gaaggattat tcttcctgca ccattttcat ccacctggat attttgataa
     1741 agataaccgg aaagtttagc gactttcatc ggcatttgtt caatctctct gttcaaagca
     1801 cttgcatcaa atggataatt tctcaatatc gaagcaatct ctaatgtacg ggacgtcgtg
     1861 gagggataca aaaagcgccc tgtatctccg acgattcctg cataaagtaa ccgtgcagcc
     1921 ttaacgttca tctttaaaga ttcaggaaac atcaaagcaa attctgcaat catctcacta
     1981 cagctacttg cttttgtatt tacccacaca agatctccat aagggtcatc attgggatga
     2041 tggtcgattt tgatcagttt ttctgccaac tgatatcttt gatcactgat tctgggtgaa
     2101 ttagctgtat cagtcacgat gactagcgct ccttggtaga agacatcttc aacttcttgc
     2161 atttctgcta aaaagcgaag cccttcaatc gtttcaccta cttgacgtat ttctttttct
     2221 gggaaacttt cccgtaatag ttcggctaat cctacttgtg atcctaatgc atcaggatca
     2281 gggcgcaaat gcctgtggat aatgatttta tcgtattttt ttattgtatc tactatttct
     2341 tgctgtactg tcatttcact cctgctttct aagaacgttc cattacttga caaacaacga
     2401 ttgctttgga aacgatggtg ttttcaataa acacttcgat atccagttta gcggaacgtc
     2461 tgccgatttc caatacacgt ggacgaatgt ctaattcgct ttccaattgg atcagccgca
     2521 aataatgtag gttcacttgt tcaatcaaga tatttcgttt ttggttcatc accattgttt
     2581 tttgtgcgac atcagaaatg atttcgctaa gtacgccaaa agaaatggtt ccaacgccat
     2641 tgaccatttg tggtgctact acgaatttga acttagggtt ttccaaacga tttccttcac
     2701 ggtcagtatc aatcgtatgg atatttccag atatctgatc cgaaatcgta tctgcaatct
     2761 gcggttgtcg ctggacaagc tgcatcgctt tcattacatc ttgccgggta atcaagcctt
     2821 ccaatgtcaa gtcatcggaa acgaccggca tgacttctag cccatcccag atcatttgat
     2881 ggctaacaga ggcaatactc attgttttct ttacactgat tggttctttc gtcatcaccc
     2941 gatcgatgat ctggtttggc gatttcccta aaacatcttt tgccgtaaca atccccatca
     3001 aacgatgatg gcggttgacg actggaaaac gcgaatgctg cgtttcttct gacaaacgct
     3061 gataatcaga aatcgtatct gtctgctgca aataatgagt cttttcagct ggcatataga
     3121 tatcactgac aagcatgata tcttttttga tcaattggtc gcttaacgct cggttgatca
     3181 tggttgcgac tgtaaatgta tcataagagg tgcgtaagat cggcatttca agatcatcag
     3241 ctagctttgc aatctcagca gtcgtattga atcctcctgt gatcaatact gcggcgccat
     3301 cttccaatgc taatttttgg acttccgccc ggttgccgac gatcattagt gatcctggcg
     3361 taatatagcg ttccatggca tgtttagtca tcgcgccgat gacgaattta ttcaagacct
     3421 tgtctaatcc tgccgccccg cctaaaacgt ccccttcaat gatcctgacg acctcgccaa
     3481 atgtcaaacg ttcaatatgt ttttttaatt tgcgttcgat acggatggtc cctactcgtt
     3541 gaattgttga aactagtccg acattttctg catctttgat tgcacggtac gctgttcctt
     3601 cactgacatt caattctttt gcgatgcttc ttacagaaat tcgattgcca acaggtaaac
     3661 tttcaatata ttctaaaatc aaatcatgtt ttgtcgccat tttctgccta cacttccagc
     3721 gtttccgaga cgctcattat ttttccttca ctatcttcca gtaactctac aaaagattcg
     3781 ggatcttgtt caatgacagg gaaagtattg taatggattg gaactacttt cttggcacga
     3841 attgtttgcg cagcctttgc tgcatcttct ggccccatcg tatagttatc gccaatcggc
     3901 aagaaagcga catcaatatc gaattcttct gcaagcaatc ctagatcact gaaataagca
     3961 gtatcccctg catgataaat cgtttttcct tctgcttgaa taataattcc gctaggctcg
     4021 cccatatact gaacgatacc gtctacttca tagcttgaac tatgctgtgc atgtacgaat
     4081 tttaccacgc cgaatggaaa cgcatgtttt ccaccgatat tcataccgaa tgaatctact
     4141 cctctgctgt tcgcataatc agcaatctca acaatactga taattgtggc ttgattttct
     4201 tttgcaatag gaatcatatc tcctatgtgg tcactatgac catgagtaat cagcaaccaa
     4261 tctgtcttta cttcttctgc agttaagtct gtcaatggat ttcctgtaat aaacgggtca
     4321 atcatcacgc gttggccatt ttccatttca attgttaaac aggaatgtcc atgatacgta
     4381 attttcatat tttacacctc gcgttttctc tgtatctttt ttattaaaaa cgatttcatc
     4441 acttgcacat caatgaaaaa atatagctgc taaatatatt ttcattggat tgatagtaca
     4501 gtatgtacaa agcaattgta acagataata caggatgcac caagtaccaa gcagatataa
     4561 tctcaagttt gaggtctctg ctctgcttag cttctcttct cgtcccttaa ctaattaaat
     4621 cagcgtaaat aggatggacg atcttttcta aatcggctac ttttatccga aggcttctcc
     4681 cgatttcacc gcttgacaca attacttctt ctttttcttt taaagaactg tctgctaaaa
     4741 aacaataatc aggatgtgcg agttttattc caataggggt gtttgctccg tggggatatc
     4801 ctgtatgcat taatacaaaa tccattccag gaaaaccaac tttacgatct ttcgtcactt
     4861 gtctcgtttt tttgtaatct aagtgatcgt tcaaaggaat caatgcgatg atcaccccag
     4921 ttttgtttcc tttcactacg agagttttac atatcaaagc tggatcaatc cctctttttt
     4981 gtagttcttc tgtgaaagat tcttctacca catgactaaa atcaagtggt tgatagctga
     5041 tcttttgttc ttctaaataa cgttcgactt ttgtttgact catcattgat tcactccctt
     5101 tattctatcg tttattataa agcgagggat gctgtattca aaaattattt gtttttcgca
     5161 aaaaaagagc aagacgagca ctctatagag aggttcatct tactcttttt gtttattttt
     5221 tatttcgacg ttgagtagct tccataatga ctggcaagat cactgggcgg cgttttgttt
     5281 gttcaaataa gtaacggcct aattgatctc ggatgtcttg ctttaatttg ctccactcaa
     5341 aatcattgct ttctaaatgt ttctcaacga ttgtttcaat catattgctg ctttctttca
     5401 tcagatcacg gctagctttt acgtaaacga atccccgtga agtaatttga ggttttgaaa
     5461 tgattttctt ttctcgccga ctgatagtga caaccgctac gaaaatcccg tcttctgaca
     5521 agatttttcg atcacgtaaa acaatgtttc cgatatcacc tatgcctaat ccatcaatca
     5581 ttacattttc tgcacttgta gttcctgcaa cactcatacg cttgttctta tattccagca
     5641 cgtctccgcg tccagtaata tagatatttt tgtatggcat ccctactgca tgagccaaat
     5701 ctgcatgagc agctaattca cgatactctc cttgaactgg cacaaaatat gttggtttca
     5761 tcaaattgat cattaattgc aagtcattag gattggcatg gccagaaacg cgcatattct
     5821 cagagatcaa cttcactgtt ccaccagctc gataaatgat atcttccgtt ttcgccacga
     5881 tggtttccat cgcgatgctt ggcgtagtag tgatataaac caaatctcct tcttcaatct
     5941 tgacattacg gtgtgtaccg tttgccattt tttgcagaga cttgatgggt tcacccattc
     6001 ggccagtttc taaaatgatc agttcttctg gatcatagtt tttcatgtct ttcaacgtaa
     6061 caagcagatc ttcacttggt aattgaagtt tttctaaacg catcgctgtg cgaataattc
     6121 gttcgaaatc ttgaccagtc aagacaactt ttcgttgtgc tttttctgct gcatccaata
     6181 cttgttgcac tcgttgcaaa ttacttgcta cacaagcgac gatgatacgc ccttcccaat
     6241 atttgatcgt atcaaatact tcatctgcta tttgggcttc agaagctaca ggctgaggat
     6301 tttctgcatt tgccgattca cttagtaagg ctaagactcc ttctttgccg atttccgcca
     6361 atcgtgcata atctgtttga tacattggga tagccgtttg gtcaaattta aaatctcccg
     6421 tataaacgat actaccttct tttgttttca gattgatccc cactgaatct ggtatcgtat
     6481 gagtggtacg gaaaaagctc actactgttt caccaaagtc tatttctgta tgctcatcta
     6541 ccacatggaa atccttaaat cctttggctg cttcattacg actgacattc aattttgcta
     6601 actcgatcgt caactctgtc ccgaatactg gtacagaaat ttttgacaac aagtaaggaa
     6661 gtgccccgat cgcatctgca tgtccatgtg ttaaaaagac accagctaca cgatcaatgt
     6721 tttcttctaa atacgtaaaa tcagggatta cgacgtcgat ccccaacaac tcattttcag
     6781 gatattttag cccacaatct aaaataaaaa tatcctcgcc cacttctgcg acatacatgt
     6841 tttttccatt ttcacgaacg ccgcctaatg ggataatttt aatggtactc acaaacttca
     6901 cctcttctta tgacatccaa gtttaaagat gtcgattttc ttctttatct aaatatatga
     6961 gccattccaa tgaagcatat gcaaaatcac tatttctcaa cttatggaga atcagcagga
     7021 aaattaccag cacatccgcg ttctgacagt tggatgggca ttttattttg gatatacttc
     7081 attaacaaaa tggatctttt gcgaaacgct atagccagaa tacttatata agtattcagc
     7141 tgttccccat tattatacaa caaataaact aaatcgctag tatcagcaat tgttagaaca
     7201 actatgcacg taaaaatccc tctgtagcat ctaggatttc ttttgctatt cctctttcat
     7261 ttcttcttcg tcggtttgaa acatactttt ttcattttac cacatttttt gcaataacta
     7321 tagtgacttt ttatatatac agaacctcat gttgatggtg tgcaaacagc ataagattta
     7381 agctagcttt aagataggtt gtctattatc aaatgcatct actaggaggg atgcaaaata
     7441 tgaattttat gaaacgtgca tggaaaagta caaaagtaaa atggggacgt tcattgttat
     7501 tgtttgccgt gttcacagcc attcttattt ttgtgctagc tggcctcacg attcgaagcg
     7561 ctgcagaaga agcagcgaat caagcacaaa aagaagttgg agcaacagtg actttaagtg
     7621 ccaatcgaga agcttcattt aaaaaacagg aacaaacttc ttcaagcggt tctaccactg
     7681 aagaacgtcc agatccaggc agtttttctc ttacaccagt ttccttaacg gacgcggaaa
     7741 aaatagctga attagataat gtaaagagtt attcctttga aagttccgcc tcagctatgg
     7801 caaaagatgg aataactgcc atcactagtt ctgattcaag tgaagacagt tcagaaacta
     7861 ctgatgcagg cggtgaacct gctggaggaa tgggcgggaa tccgcaaatg actcaagcag
     7921 actttcaaat cagtggtgtg tcagcaactg cacaaaatag taatttttct gatggaacag
     7981 caaaaatcac ggatggtgaa ggaatagatg acagcgacaa aggcacaaac aatgtagtca
     8041 ttgattctac tctagcagaa acaaatgacc tggcagtcgg agatactttc acaatcacga
     8101 gtactgaaga tgaagatacc acttatgaaa tgacgatcaa aggaatttat gaatccagcg
     8161 aaacaagcgg cagtatgggt atgaaattca acttcatgaa tccgtcgaat acactttaca
     8221 cttcttacac ttttgcaaat gaactaaatg ggacatctga cgataacacg atagattctg
     8281 ctatttacac tctatctgat ccagataaaa tggatgagtt tctcaaagaa gctgagaagc
     8341 tgatcgacac agatacattt agcttacaat caaatgattc catgtatcag tcaatgttgg
     8401 aacctttaaa caacgtggca agtttttcta aaaatgttgt tttactagtg gctgtagccg
     8461 gtattatcat acttactctg atcattatga ttaccattcg ggagcgtcgg catgagttag
     8521 gcgtcttgct atctttagga gaatccagaa gtaaaatcat tctccaattg tttaccgaag
     8581 tggctatttg tatgattctt gcattaggag ttgcttcgct tagcggaaac gtcgtcgcga
     8641 atgccgtggg acagcagcta cttgaccaac agacagaaac cacgacacaa aaccagcaag
     8701 ctcctggcgg tggacaaatg ccaggtaaca gacaagaaca aggcggtcaa cgcgatggtc
     8761 aacaaggaac cacgaataat ccatttgctg tttctgatca agtcagcgaa ttggaaatca
     8821 atgtcaaacc tgcacaattg ggactattag ccggagtagc atttggaatc agtctgtttt
     8881 ctgtcttttt agcttctata gggatattaa gattaaaccc aagaaaaatc ctattaaact
     8941 aaaggaggaa tttgatgtta cggacagaac aattgggatt ttggtatcaa gatccgcaag
     9001 atgcgcttta taaagatgta gaactgaatt tcgaagctgg aaaaatgtac gctattttag
     9061 gagcaagtgg ttctggaaaa acgacttttc tttcgcttat ttctggacta gatgcaccaa
     9121 aagaaggaaa ggtatactat caagatcgtt cactgaacaa aatcgggtta agaagttatc
     9181 gtaaaaatga tgtctcgatt attttccaag cttacaactt gcttccttat ttatcggctc
     9241 tagacaacgt catagcagca atggaaatca ctaagaccaa gcaagagaat tatcgacaag
     9301 ttgctttaga tcgactggct cgtgtaggaa ttgatgaaat attagcaaaa aaacaggtga
     9361 ccaaactttc gggaggtcag cagcagcgag tcgctatcgt tcgtgctatc tgctgtgaac
     9421 atcaactaat cgtagcggat gagcctactg gaaatctaga tgaaacaact tcacaagata
     9481 tcgtcaaact tttccaagaa atcgcccatg aacaaaatcg ctgtattatc atcgtgactc
     9541 atgagattga agtagcaaaa agctgtgatg aagtctttga actaaaaaat aaaaaatttc
     9601 aaaaaatcga agtttaaaaa acaggataat gatcaaataa aaaatccgaa ccatatctgt
     9661 ctcgcagata tggttcggat tttttatttg cttgattcaa ttagtaataa gtggttttta
     9721 ttttttttcg tcttttcgag aaaacttgtc tttgacttcg tcgcctaaat ttcgagcttt
     9781 ttctttgatg tcagcaccta gtgattgtgc tttcccttct gtttctttgc tcttgtcgtc
     9841 tgttgctttg ccgtatgctt cttttccttc accgactact ttatctttca tatctttaga
     9901 acgtcctttt aaatctgcca ttgtgcttcc tcctcttagt ttgtttattg atctaccttc
     9961 agggtaacat caacaacaga tacagacaaa cagaacgctc gttttctaaa aaagatagtt
    10021 agagctgttg attaaaaact tttttcgaaa gtcccccata ctttgacttc tttttcttgg
    10081 accattattt ctcctttagc gataactgta tcaatatcaa gcgtttcttt atccaagata
    10141 cacagatctg catcagcacc aatttcgatt tttccttttt tatccaattt caatatacgt
    10201 gctggattac tggttatagc tcgtaaggcg atttccaaag ggatactttc tttttgcaca
    10261 gcttctttga tccctactag caatgccttg gcacttccta ctcctaaccc taggaaatga
    10321 ttgttttcat caaaataagg caagcttcct tgtccatctg aggtcatcgt gaaattatct
    10381 aaagaaacat cttcctcgat caatcgtttc agtgctttgc tgaagcgaac ttcaccatct
    10441 gtcttctccc aaaaatcagg atcttcgcta gcagtgaagt caatcgtgcc accacgtttg
    10501 gcaaatgcca cgcaagcttc aaataattct ggtgtgcgat ttgcatgagt aggcaaaaat
    10561 tgtgtgatcg gaatttctgt ttcttctacg attcgtgtca atagctctag ctttcttttt
    10621 cctccgccta agtggacatt gatgatccca gcttttccgg ataacatgcc tcctacacga
    10681 gcatctgcta ctgcacgagc gaactcttca aaacttggct gagaagaacg gtgatcggat
    10741 actgcgattt ctccgattcc gataatttta tcaattgtca aaaagtcttt gatgatagaa
    10801 tctgttactg tttttaccgg caatcggtac gaaccttcgt atacataagt ggagattcct
    10861 tctgcttcca gacctttcgc tttgctgatc aacgcagtca tatctctacc ttctccgtct
    10921 gttcccaaac aaccgacaac tgtcgttaca ccagctgtag tcaattggct caatgtgact
    10981 tctggcgttc gattttgata accgccttct cctcctccac ctaaaatatg gaaatgacag
    11041 tcaatgaaac ccggggttag tacttttccc tcacctgaaa gttcttctac ttccactcct
    11101 tcgtagccgc cttctagctg ttcttctaca gccaaaatct tcccaccggc aatcagtaca
    11161 tccatttttc ctaagtaatc tggtgcaaac acttcaattt gtcgaatgat tttcatacat
    11221 ttttcctctt tctttaagta taactgatga taggatctta aaaaataatc caaacgatag
    11281 gatctttact cctttgattt taccgtaaaa tgacggttct tggagtcctt atctactgtt
    11341 tggattactt ttttatttac aggtctccag tactactgca gacctattgc aatggcaatc
    11401 atgatgaaaa tagttcctaa aacaaacagg aatccttgca tcttgatttg gaatcttgcc
    11461 cattttgccc attcgatccg tgcaacacct agcacaccaa tcaagctggc tgatacgggt
    11521 gtaaaagcgt ccataaaacc tgaaccaagc tgataagcaa gaactgctac ttgtctggat
    11581 acacctacta aatctgccag tggagccatg attggcatag tcaaagctgc ttgtcctgaa
    11641 tttgatgtaa caaccaagtt gaacaaactt tggaagatgt acatcgccca agcaccaatg
    11701 acagctggaa caccggtcaa cgccgtcccg atcccatgca aaatcgtatt caaagctgaa
    11761 ggaacagatg catcagatcc tcccaagaca agaagaatcc ctttggccat acctactacg
    11821 attgctgtac cagctaaatc agctgcacca gattggaaag aagaagcgat gtcattgact
    11881 ttcattccat ctaatttgaa aagaacagca atgattccag caactaatcc catcacaaag
    11941 aattgagaag cgatttctgg aatgtaatag cctttttgag tcacgcccca aacgatccag
    12001 acaaggacaa ctaacatctc taataagatc aatttatgcc caagcatgaa ttttttattt
    12061 tcatcagatg tctttttgat atgagaacgg aaataatcat ctgatttgta agtcaacgaa
    12121 ctttctggtt ttttacggat tttttctgca tatatcatca tataaccagc ggctaatgca
    12181 gtaacaacta cccacataat caatcggaat gtcgcaccag acaacacggg gattccagca
    12241 atcccttgag ccaccgctac gctaaacggg ctcatccaag aggtcgcatt tccgacttga
    12301 gatgcgacat aagtcacggt tactgctacg atcgagtcat atcctaacgc aatgacaaat
    12361 ggaaccatca ccattgagaa tggaattacc tcttctgcca tcccgaatgt agcgccaccg
    12421 aatgaaaaag caaagaataa tagcggtaaa gctaaacgct ccagtccctt agttttactg
    12481 ataaacgcat agatacctgc gtcgatcgct ccggtacgca taataatacc aaaagcacct
    12541 ccaacaacaa ggatcaatgc agcgatccct acagcagatc catacttatc tccagatact
    12601 agtccttcaa aaacaaaatt taagaaacca aagccgccaa aatcatctgt tccccatatt
    12661 ttggctgttt tatgcaattt tttagaagta tcatagatat tttcgccgta ctgatcgtaa
    12721 agcacatcat ctgtcaagga gatttcatct agtttttcct gcgtaagatt tttttctccg
    12781 tcagcaatga ctttttccaa tcctttttcc ggaacatcca gtttttcacg ttctgctgga
    12841 tgatcttgca gttcttctaa ttgatcaaac acatacgact tatccagctc gtaagcataa
    12901 cggaaggaat cttgacgtaa aacagtacga gtacttgttt cgccatttgc atctttgtat
    12961 tcaatatctt gcgtgctgaa ctttcctgct ggaacgacaa aagtcagtat ccatgcaaag
    13021 atgactacac caaaaataat gacatacgta tggggtgtcg ataatttagt gaagtcgcgt
    13081 ttcggttttc ctgaagaatt tgttttcttc aaatttattt cttcctttct tttttcagta
    13141 attttgatga ataaaaaatg gataatcaaa tgaattatac agtaaaaatg acatttccac
    13201 atgaataatt tattagaaaa tcactttttt ctattttgtt ttttttctcc tatgtatgat
    13261 tatttttttc tctttccatt tatattaagt attttatttc tctgtttttc cttaaaattc
    13321 tttttttatt tttagattag agaaaatctc gtcctgtttt tttcatttta caatcattga
    13381 aaaattgaat ttcattgcaa aaaaaataat aaacacttat aaatatccat tatctatcct
    13441 tcaatttttg tgtattttat ttctattaga aaaaggtatc ctgttggaca attttgttat
    13501 atcaggtaaa cttcattgca ataatgataa caaatgtact ataattactt tgtaaagagt
    13561 tttggggaag actctttcat caacgtgtat taagagacaa gaaagaatcc atatggattc
    13621 tttcttgtct ctttttccac tactttttat ttttctgaca ctacatattc ttataactca
    13681 tttctcataa ctgtgaaaca acttataaaa ataacaaact ttaaacaaaa caagcttaaa
    13741 atcaaagatg atccatctta aaataactaa aacaaaaaaa gtagtcgttg ttaatcctca
    13801 ttaataatcg ttttcttttt ttcaaacgct ccatttttat gtggaacatt atttttttaa
    13861 ggaactttgt caacatgtgg ataactattt tccacttgtg tcctggacta gctgttttgc
    13921 tgtaaaatac aaaacttgct gcagcaaaaa gatcgttgca agagaggctg ttccgcagat
    13981 cgctggatct agatttggca aagtcaagac tgcatcatgt aaattggcgt ggttgtccaa
    14041 aaccaaatca gcaaattcaa ataatcgcaa accactgcta tgttgcgatt tgatacttcg
    14101 tgacaaatca aatccagtaa caatgattaa tggatgacca ttttctttga tccactgagc
    14161 tagttcgatg attgccgggt ttcttccttc attcgaaaga agaagaaaaa tgtcttctga
    14221 tttgacagaa agctgatcaa taattgcctg tcctgcacct tctagcgttt catagacacc
    14281 tccggctgga ttttcaatca atatacttgg tacaagatcc ggggtttgat cccaaaattc
    14341 aaatgctaca ccacttagtg tgcggctagc atatatctgc aatctaccac cttgattaat
    14401 ttgtttcgcg atccaagtgc cagcttgaag catcagcggc tcttctatcc ttaatttctc
    14461 ttccaattgg tgataatagt caaaataaca taaactcatt tatctctccc gcctagctat
    14521 acttgacatg aatagaaaca gctgcctaat ctaaggaagt acataaaaaa acagtagtta
    14581 gacagtaaca catgtggtct ttgctgtttc aaacacaaaa aaggcattga acgaatacgc
    14641 ccaatacctt gacatagcaa ataaaacaac gtgcagacca tggaaaatat ctacataggg
    14701 gtagaatttt caccctagat taggtacaat aaattaggtc gatgcactgc attgactatg
    14761 gcaaaatgat cggtcagggt gtatgtgtgt gactggtcat tccctgattc ttagcgccaa
    14821 ctaagggtca gggagccttt ttttattcag ctgtttctat ctgaatttat tgtaagtaat
    14881 ctcttatcca agtgcaagca agaaaataca aaaaaacatt ggtatgattt atatatatat
    14941 aaataacata aaaaaaggaa tggcttatcc cgatacaaca agcattcctt taaaaaagat
    15001 tcttttatat aagtgaaaaa agcttttttc caccaattaa ataaatctct catctcaata
    15061 gtttatccat tattttatct ttttttgcgt atacctacta aatattaccg tcttcttctc
    15121 gttcttctac tttgttcgag agaatctgta ctagaaaaag tagttgtatg tctacctttt
    15181 ctgcctatcc gagactttct tttcatatat gggatataaa tgattagata cagtacagca
    15241 aaaaataaag caaatacgca agcttcccaa ctacctaacg ccaattcaac catcaaggta
    15301 gcaaccccaa cggatgctgc ataccagata aaaatcagaa acgtttcttt ttttctgacc
    15361 atgcgaaaca tgataaatag actaataagc aatagtggca acacaaatat ccaattgctt
    15421 ccttccatca gccacttgag tatatcaagc agcgactgtg caacagcgac taattttccc
    15481 atagtgaaca cacacctttt tttataatta aaaaattcgc ctttttattc tactatgaat
    15541 cttctggctt ggctttcatt tctcttatgt aatcattttc ttttatacaa cttaaaaaaa
    15601 aattaaacca ttagcataat attttctgaa tctattcacg aaagtctttt tctatctttc
    15661 gttttccacg actcaactga tctagagtca aaaaactaaa acgacagttg cgcaaatgac
    15721 gcacaagctg ccgctttaat aacaaatagc ctttcattct cagcaggcca cttatttcca
    15781 gttaactttc tgttcttagc tttttactca actggataat atatgcactt aatgaagtcc
    15841 caatttccga atgttgaagt ccatattcaa tatttgtctt catatatcct aacttatcgc
    15901 ctacgtcgta tctttttcct ttaaactctc tcgcatagac cgcctctgtc tggttaagag
    15961 tctgaagtgc atccgttagc tgtatctcgt ttcctgctcc tggttcttga gtttctaata
    16021 tctcaaatat tttaggagtc agtagataac gtccgataat ggctaaatta cttggtgctt
    16081 ttcccatttc aggtttttct acaaaccctt ttactctgca acgacccttt tcgctatctg
    16141 ttttaggatc aataattccg tatttagatg tttcattttc tggtacacgc atgacggcaa
    16201 cagcggaagc ttgtgtttct tcataatcca tcatcaattg ttgagtcaat gggatatcat
    16261 ctttcatcaa atcatccccc agcatgacga caaacggttc atttcctata aatgcttttg
    16321 cttgaagcac ggcatccccc agtcctcttg gatgagattg acggatgaag tgaagattga
    16381 cttgagtcgt ttcttcaact aaagcgagta attctgtttt ttccttttcc cgcaaattgt
    16441 tttccaattc gatatttgca tcaaagtgat cttcaatggg tcttttttct tttcccgtta
    16501 cgatcaagat gtcttcgatt cccgaatcca atgcttcttc cacaataaac tggatcgttg
    16561 gtttatccac gattggaagc atttcttttg ccatggcttt tgttgccggc aaaaaacgtg
    16621 ttcctagccc agcggcagga atgactgctt ttttgacctt cacttgct
//
LOCUS       DARBET010000056        16139 bp    DNA     linear   BCT 07-NOV-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4027
            SAMEA114569841-rid20112183.denovo.058, whole genome shotgun
            sequence.
ACCESSION   DARBET010000056 DARBET010000000
VERSION     DARBET010000056.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA114569841
            Sequence Read Archive: ERR12203666
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 16139)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16139)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001972618.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001972618.1
            Genome Coverage       :: 132x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/07/2023 13:18:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,041
            CDSs (total)                      :: 2,975
            Genes (coding)                    :: 2,908
            CDSs (with protein)               :: 2,908
            Genes (RNA)                       :: 66
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 67
            CDSs (without protein)            :: 67
            Pseudo Genes (ambiguous residues) :: 0 of 67
            Pseudo Genes (frameshifted)       :: 26 of 67
            Pseudo Genes (incomplete)         :: 28 of 67
            Pseudo Genes (internal stop)      :: 20 of 67
            Pseudo Genes (multiple problems)  :: 6 of 67
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16139
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA114569841-rid20112183.denovo.058"
                     /isolate="VRE4027"
                     /db_xref="taxon:1352"
                     /country="Denmark"
     gene            complement(<1..258)
                     /locus_tag="SCJ86_002017"
     CDS             complement(<1..258)
                     /locus_tag="SCJ86_002017"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEG1169551.1"
                     /translation="MERQESIREVQEVPTEQAKETQEGRSFSYGKNFSFLRPSIQISV
                     FVLWRIHNNRYSVGSQLFYQEICHALSKKYSQNAYKEALAFL"
     gene            complement(274..924)
                     /locus_tag="SCJ86_002018"
     CDS             complement(274..924)
                     /locus_tag="SCJ86_002018"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347557.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEG1169552.1"
                     /translation="MKKRFIYVCVGGLFLFGVGSTFFLIHEKLQLTAKEEKLTVEINK
                     FQEEKKGQEDLVTSLKEQLQQVESEVTTLKKANEELKNNDSTEKGNIQTFQKTVTEAF
                     EVLFNYEPDSFQERKNNCAPYLSDKLMKQYFKEGVSYGDSNGVSSELEKCSVYLKSTQ
                     KATIQGLVVVSYRNKIGDSSWNNAMNIFDVTFDPASKKITFMQNLGSSFNQQLLEE"
     gene            complement(935..2107)
                     /locus_tag="SCJ86_002019"
     CDS             complement(935..2107)
                     /locus_tag="SCJ86_002019"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347556.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CHAP domain-containing protein"
                     /protein_id="HEG1169553.1"
                     /translation="MKKKLLLWCGFGCLPTLLLCMFGFLFMILLISSGSEEEEIDCSP
                     TEIVASTISLTNQGMETNGLQIAKEVQKHIPNTTLEGLAGMLGNFQQESSMSPTAIER
                     PNDPLSGHGIAQWTAGRTTALKNFAKERNKEWSDLGVQVEFLIHELNTTEKASQVALQ
                     AKSVEEATEQWQVKFERAGIPMMGNRITFANQWYAKLGKNDSDPVASITLENGANAGS
                     KDLTDYCLETTGTSSSSDSNVLNVAKSLIGYFHYSQPQRAQFGSVENPDKNGYADCSS
                     FVWLVLTKAGYKTPANVGWFTGSMTSDARGSKQYLQEIPESEAKAGDIIIVNLGSGVG
                     NDGHTAVLAEDWHGLSTKVIEQGGMNTNGVGEGQVNLSFGYLLNGGDICFARPIKK"
     gene            complement(2126..2998)
                     /locus_tag="SCJ86_002020"
     CDS             complement(2126..2998)
                     /locus_tag="SCJ86_002020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347555.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LPXTG cell wall anchor domain-containing
                     protein"
                     /protein_id="HEG1169554.1"
                     /translation="MKKMILGSLFSATVLLGMVGTSAYADTTSPGDPSVPPTEIIPPT
                     EETPEVPEEPVLPPTDGGEETPEIPEIPETPETPEIPPTEENPDTGGGTETPEVPENP
                     DTGGGEGTPEVPPVEENPGNGGNTETPEKPSNPGGNEGNTTPPTTPPTNNGGTNTTPP
                     VQDVVVKPDGSIGSQQTGGQNVQIETNNASELTHIPTPSTPIETASGEKIVSVVDGVA
                     YKESGNGLTPISDKVEQLPSGNIAVKGSDGKMKVLPKTGEEVSLVATALGGLLASGSG
                     FAFWRKRKATQKMN"
     gene            complement(3020..3940)
                     /locus_tag="SCJ86_002021"
     CDS             complement(3020..3940)
                     /locus_tag="SCJ86_002021"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347554.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEG1169555.1"
                     /translation="MMMIRFTEVDNMSLLTDKKIALDKAFIQLKRENEELLARAEIGW
                     CTLEIYTENHEIPVFEGSYDFPVEEFHLISLIEQSLTENGQLSEEAQTFIQTLNSELP
                     KFYQVKKDQKNERRPTKIKKEKKTPLSVETAFTQSNGSLQKKKMSFQWLLFGFIVVVS
                     CGLFIMQLSTQREIEQLTKKEHQLQERIAFAEELATKQPQIDTFSRYFITNYYSGEMA
                     EERYRKKLQSFLTEKVAKQISPQKEKVKSILSWEMKKEGKHWQIAYVITLETEKKTQE
                     TKKVSFQLEEQEKNYLVSTLPELDDFEINQ"
     gene            complement(3940..4209)
                     /locus_tag="SCJ86_002022"
     CDS             complement(3940..4209)
                     /locus_tag="SCJ86_002022"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347553.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEG1169556.1"
                     /translation="MIIVTGIVLLGIGVLFGYHFAYFPKIGEWQQRQAIQKYFQVKGN
                     EYTYYAEDEDSYIVSLNNQEYRVKFSLNNPIQVVYAVEVVEEVFD"
     gene            complement(4206..4586)
                     /locus_tag="SCJ86_002023"
     CDS             complement(4206..4586)
                     /locus_tag="SCJ86_002023"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016632446.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF805 domain-containing protein"
                     /protein_id="HEG1169557.1"
                     /translation="MITNYKKFWQNSFNYKGKATRKEYWLVALMNALIGFGLGLAIGL
                     AGALNASETIIGCLIGVLIVYCFVVLVPSLSLSVRRLRDAGFHWAFIFFYFVPTIGSL
                     VLLVLYCLPTKESIEDKQVQEETR"
     gene            complement(5053..6516)
                     /locus_tag="SCJ86_002024"
     CDS             complement(5053..6516)
                     /locus_tag="SCJ86_002024"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347550.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan DD-metalloendopeptidase family
                     protein"
                     /protein_id="HEG1169558.1"
                     /translation="MKKTSKCLSALLLVGQVFGYTPAVLANEQGNNQLTNSQSATITE
                     ETTTNSTGEENQPETPTEDTIDTPDNPEMNAPPTGSEEQPETPNQPDKPEDNQQPEEE
                     PSEEPNEQPSDESSQTPQKPSTETEAEPPTKTERPQKAPEQSVTVPMNDVPQAQSIGS
                     EETKNYHQAPSAPIQELVPSSTQDNEGTIHFEKDASVETFIRKIGESARKIGQENDLY
                     ASVMIAQAILESASGQSQLAQAPNYNLFGIKGTHNGKGVSFATQEDLGNGTLYTTQAT
                     FRQYESYEESLKDYAKLLKEGLVGNTSFYDGVWKSKAKTYQEATKFLTGRYATDTSYD
                     KKLNGLIETYDLIKYDKEVAGPQLSKEGYMVPLKNYSISSSFGWRGEEFHRGLDMAAN
                     QGEPIYASKAGTVIEASYHTLSGNYVTIEHEDGSVALYAHQQEYCVNVGQTVEQGQII
                     GYVGSTGNSTGPHLHLELALDGSLTKDKLINPETVLF"
     gene            complement(6580..9597)
                     /locus_tag="SCJ86_002025"
     CDS             complement(6580..9597)
                     /locus_tag="SCJ86_002025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347549.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LPXTG cell wall anchor domain-containing
                     protein"
                     /protein_id="HEG1169559.1"
                     /translation="MFKRMATWLAIFVTFLNPMLGSVTAFAETINESNSTEEVMKTEE
                     SKSSEEAISTETTSETTEETTTQDTTEDVSPPNVMTQEVPEGYEKYFITIEDIQKATS
                     FSEQERLAQESEEWQAILEKNAQTRATNLTLTENGRKDVNMTRITPEGTMYTHDYMVR
                     WQIDGEDVFCIQEGAITSAGIQYDVTPLENLITTSAIRKKISLIGYFGYYSSGSMDNY
                     VLAQTYIWEVLGGSFVNYGTIGQSNYEAFKQTVDRKINNLLEDPSFDSQTVTVKVGEE
                     VEVKDTKGVFSQWDGSVVENTANVQVRKSGNSLFIKANQNSNDGRISVSSLDPNSRGV
                     GSSFAYVDPVAQNLGKLYVPDPNRAGLTIKVLKNGDFKLTKLDAVTGKKVPNVTYEIK
                     RHDTGETKQYTTDAKGEIALKEIPAFYEKDGKYVQNKLDVKEVKAPAGYILDKQTHTI
                     TIEPNKEVTLTAKNDLQQFIPEGKKVKEVYNAEESWNQGKPVYDTVPAENIEFDIIAK
                     TDITAPDKETVLVKKGEVLDSVKTDKDGHWKANKKFYNGDQNKYQLIEKNVPDNYRQP
                     SEEQTTFSVAYGDNTTPVLTFELDVIDNLLKTGEVDFNKVDSINHLQLPGAKFLIEGM
                     TAHNKNVSFVYETKEEPEKLKLPDGTYKFTEIVFPEHYGQASGQTETILVTVKDGEVQ
                     PITVKNEEIVVPEEDQPEISTLFVTTTGGKDFNPKKDQELVDKVSQVFPKSAVGTEKY
                     WVTQFHKITADGKVTVLDTVKETRKVTDEKETFDVTFNYKADMVSDGDTIVATHIVYN
                     DKEEEEEFARHFDLTNKDQTVTAKEPKIETRFITVNGDQTFDPDKDQELIDHVSQEFP
                     ESTVGETKYWVTQFHKIDKNGKDTVVGTVETARKVSKASEEFKVSFDYKAGTLKDGEK
                     LVATHIVYKDKDHKEEEARHFDLKNEKQTLTAKGIKKETPTTPVKQTPTSTPKQSLPK
                     TGETVNDVLIALGLFLFVGVGAVLVRRKMKQSN"
     gene            complement(9748..10587)
                     /locus_tag="SCJ86_002026"
     CDS             complement(9748..10587)
                     /locus_tag="SCJ86_002026"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334213.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysM peptidoglycan-binding domain-containing
                     protein"
                     /protein_id="HEG1169560.1"
                     /translation="MKMLKVLALGIVVGGVSLAVGTNAEAAENKVWTPRSAEQIKAEI
                     KGNEYTIVWGDTLSAISQATNITVQKLADMNKITNVDLIYAGNKLVFNGNVASVQNSA
                     GETVAQTVIQEKDKVDPNQPVGQGTTSNTQTTPNTTTPTTPAGDQTGTTGGTTTPSTP
                     ENNGGTTQTPTQPGNGGNTGGETTPSNPSTGGGTTTPEEPSNPGGGDQGGNGGTTTPE
                     EPTIVVGEIGNSGKVFNTVDEAYDWANAEVSDPNSQWYNKGFMVDVIIYSDGSQKHTV
                     DFY"
     gene            10732..10977
                     /locus_tag="SCJ86_002027"
     CDS             10732..10977
                     /locus_tag="SCJ86_002027"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014862476.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEG1169561.1"
                     /translation="MQLNNLNEIHDWAQNNLLNRKQAAKITGQNYDAFSSSLRRNNIK
                     PFLEIGDEPGPSIIRLYLREDIEKYAKQLEEKRQKRQ"
     gene            11040..11462
                     /locus_tag="SCJ86_002028"
     CDS             11040..11462
                     /locus_tag="SCJ86_002028"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014862359.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEG1169562.1"
                     /translation="MNKKYIVSVGSIVLIVLAIIAFSVSNKTETQPQNTVQSTTTSQS
                     SDHSVYPSLNSLKETHGQDLIDLGSSQITTVLFEKKNIAFITPKEGTDLEFSIKVTNT
                     KENLFSIPDIQYTPNKLKIGDTFGLAKVNEQYFAYSLK"
     gene            complement(11556..11873)
                     /locus_tag="SCJ86_002029"
     CDS             complement(11556..11873)
                     /locus_tag="SCJ86_002029"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEG1169563.1"
                     /translation="MPVKGTSNFDFEIFYVDDEICGVFNKQKYTKEQAIEAWKNELFD
                     PEEEISFVVDESFVGWYHGNAEEEEVHYAGWWLEEGEPREQSVPVWVISENPLEEIEK
                     LEK"
     gene            complement(11900..12106)
                     /locus_tag="SCJ86_002030"
     CDS             complement(11900..12106)
                     /locus_tag="SCJ86_002030"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEG1169564.1"
                     /translation="MNWLKNEAELYVFHEEMQEKHLVPNVRNYLFWLEEKNDAQVFGK
                     SAQSPKIKLQLTSKQWNEHEWYIY"
     gene            complement(12087..12269)
                     /locus_tag="SCJ86_002031"
     CDS             complement(12087..12269)
                     /locus_tag="SCJ86_002031"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEG1169565.1"
                     /translation="MTKCERVSVTISFIALLLSVISLLNTAIFIGQVEKQKEISLSEW
                     RYEEVNEGMNNELVEK"
     gene            complement(12283..12633)
                     /locus_tag="SCJ86_002032"
     CDS             complement(12283..12633)
                     /locus_tag="SCJ86_002032"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEG1169566.1"
                     /translation="MKKSYKVFENNEIKEQEAIILSAQTPEEALEQALPFLVESQNIV
                     VEELPEFDSKIVFSFNCKKESEISVSPEFYCRSDISVEGLVMCASAFIEIFANHLQIS
                     VKELLDLLQEITNE"
     gene            complement(12659..12961)
                     /locus_tag="SCJ86_002033"
     CDS             complement(12659..12961)
                     /locus_tag="SCJ86_002033"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347547.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEG1169567.1"
                     /translation="MLSKQEKKYIRQKMYAMIGNRAITELSSEELLQVQQLAQIIGSN
                     SIFDSNPLPEMTLETLTAKRYKELRAIGYRMIDIRRALRISDTKFREWRTSNGLAN"
     gene            complement(12964..13197)
                     /locus_tag="SCJ86_002034"
     CDS             complement(12964..13197)
                     /locus_tag="SCJ86_002034"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEG1169568.1"
                     /translation="MTISVGNKVKYIGTAIPRYTNKLVVVKNILVNGLILEYPKEDRR
                     KVVVEGNGTWNMNSFICGFTEVEVTNDNENRSG"
     gene            complement(14183..14505)
                     /locus_tag="SCJ86_002035"
                     /pseudo
     CDS             complement(14183..14505)
                     /locus_tag="SCJ86_002035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347542.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(14628..15743)
                     /locus_tag="SCJ86_002036"
     CDS             complement(14628..15743)
                     /locus_tag="SCJ86_002036"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347541.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiator protein A"
                     /protein_id="HEG1169569.1"
                     /translation="MATKRFTATRKYNELYYQFPKVFITSEKYRSLSDKAKIAYMVFR
                     SRLDLAIQRNQVDSQGFVYFEFTEQELANALNCCRRSVNTIKKELKEYELLEIEDMGF
                     DQQTGKRNKARLYLGELDTTENDVYSLGPVDSTSVDSAQGKEEEESVDNLAIFDDFDE
                     KNPCENSAQLFNNSSNNLDTENLDTKIDTTSPVEMADDWQKEFIAHYSESTFVPKDVL
                     TWIVRSSAEWREAKEQLNMIYDSKRIIEKRQRKYNQTHGLSDGSEYRILGETFAEDLS
                     KTYQRCIQNEKIARHEGKAIQNRKGFYFRALLNFWECCVRELNLDPMATKLSDQAEYS
                     LLQAKRSCSYEDLETQIYGLSQLPKISMHNWLEELDT"
     gene            complement(15749..>16139)
                     /locus_tag="SCJ86_002037"
     CDS             complement(15749..>16139)
                     /locus_tag="SCJ86_002037"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347540.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="replication initiator protein A"
                     /protein_id="HEG1169570.1"
                     /translation="NKFRATDVLTQATFHMPKLFFIKSSKYYAMTAQAKYAYSLLNEY
                     LEESLIEKRIDEKKHVYISYTNQELAILLNVSEEEIEEIKNELEDYDLLTIEFGRIYL
                     KELIATMEDVRVYQHQNSFLIYGQDEV"
BASE COUNT         4253 a         3387 c         2538 g         5961 t
ORIGIN      
        1 caaaaaggca agtgcttctt tgtaggcatt ttgactgtat ttttttgata aagcatgaca
       61 aatttcttga taaaatagtt gactgcctac cgaataccga ttattgtgta tccgccacaa
      121 aacgaacaca ctgatttgaa tcgacgggcg taaaaaagaa aaattctttc cataagaaaa
      181 agaacgtcct tcttgggttt cttttgcttg ttctgttggt acttcttgta cctctcttat
      241 tgattcttgt ctctccataa ccttctcctt ttcttattct tctaaaagct gttgattaaa
      301 gctacttccc aaattttgca taaacgtgat tttctttgac gctggatcaa aggttacgtc
      361 aaaaatattc atggcattat tccaggaact atcaccaatt ttattgcgat acgacaccac
      421 aacgagccct tggatagtcg ctttttgtgt gctttttaaa taaacagaac atttctctaa
      481 ttcagaagac accccgtttg aatcaccata ggaaacgcct tctttgaaat attgcttcat
      541 cagtttgtca gaaagatatg gtgcacaatt gttttttcgt tcttgaaaag aatcaggctc
      601 ataattaaac agaacctcga acgcttctgt cactgttttt tgaaaggttt gaatgtttcc
      661 tttttctgtt gaatcattgt ttttcaattc ttcatttgct ttttttaacg tcgtgacctc
      721 gctttctact tgttgaagtt gttctttgag tgacgttaca aggtcttcct gtcctttctt
      781 ctcttcttgg aacttgttta tctccaccgt cagtttctct tcctttgctg ttagctgtag
      841 cttttcatga ataagaaaaa atgtactgcc tacaccaaat aagaacaatc cgcctacaca
      901 aacataaata aatcgtttct tcatttgaca cctcttattt ctttatcggt cgagcaaaac
      961 aaatatcacc accattaagc aaatacccaa aggataaatt gacttgccct tcacctacac
     1021 cgttagtatt cattccccct tgttcgatta ctttcgtaga aagtccatgc caatcttccg
     1081 ctaaaacagc tgtgtgtcca tcatttccca caccagaacc taagttcaca atgataatat
     1141 cgccagcttt tgcctcgctc tcaggtattt cttgtaagta ttgcttactt cctcttgcgt
     1201 cgctcgtcat tgagccggta aaccacccaa cgttcgcagg cgttttatac cctgctttcg
     1261 ttaaaacaag ccacacaaag ctcgaacaat cggcatagcc atttttatct ggattttcga
     1321 cactaccaaa ttgtgcccgt tgcggttgtg aataatgaaa ataaccaatc agactttttg
     1381 ctacgtttaa tacattacta tcggaagaac tgcttgttcc tgttgtttct agacaataat
     1441 cggtcaagtc tttacttcct gcgtttgccc cattttctaa tgtaattgaa gcgacgggat
     1501 cactgtcatt ctttcctagt tttgcgtacc attgattcgc aaaagtaata cgattcccca
     1561 tcattggtat ccctgcacgc tcaaatttca cttgccactg ttctgttgct tcctctacgc
     1621 tttttgcttg taatgcaact tgtgaagctt tttctgttgt gtttagttca tgaatcaaaa
     1681 actcaacttg tacacctaaa tctgaccact ctttgtttcg ctctttggca aaatttttca
     1741 aagcagtcgt tcgccctgcc gtccattgtg caatcccatg cccagataat ggatcgtttg
     1801 gtcgttcaat cgctgtggga ctcatactcg actcttgttg aaaattgcct aacatacccg
     1861 ctagaccctc taatgtcgta tttggaatat gtttttgtac ttcctttgca atttgaagcc
     1921 cgtttgtctc catgccttgg ttcgttaatg agatagtact tgcgacaatt tcggttggac
     1981 tacagtcgat ttcttcctct tcagaaccac tactaatcaa aagtatcata aacagaaaac
     2041 caaacataca tagtaatagt gtgggtagac aaccgaaccc acaccataaa agaagttttt
     2101 tcttcatgtt tgatccttca ttcgcttagt tcattttttg tgttgctttt cttttgcgcc
     2161 aaaaagcaaa accgcttcct gaagccaata atccaccaag tgctgtcgca acgagagaca
     2221 cttcttcgcc agtcttaggc aatactttca tttttccatc agaccctttc accgcaatat
     2281 ttccgcttgg aagttgttcg accttgtctg aaatgggcgt caagccattt cctgattctt
     2341 tataagcgac accatcaacg acggagacaa ttttttctcc tgaagctgtt tcaattggtg
     2401 tacttggtgt tggaatatgg gttaattcag acgcattatt ggtttcaatt tgaacatttt
     2461 gtccacctgt ttgttggctg ccaatacttc catctggttt tactacgacg tcctgtactg
     2521 gaggcgtagt gttcgtcccc ccattattgg taggtggtgt cgttggagga gtcgtatttc
     2581 cttcattgcc tcctggattc gacggctttt ctggtgtctc tgtattacca ccatttcctg
     2641 ggttttcctc aacgggtgga acttcaggtg ttccttcccc tccgccagta tctggatttt
     2701 ctggtacttc aggcgtttct gtgcctccac ctgtatctgg attttcttcc gttggtggaa
     2761 tctctggggt ttcaggagtt tctggtattt cgggaatttc tggggtttct tctcctccat
     2821 ctgttggtgg taaaacaggt tcttcaggaa cttcaggtgt ttcttccgtt ggtggaataa
     2881 tttctgtcgg tggtacactt ggatcacctg gtgacgtagt atctgcatac gcactggttc
     2941 ctaccatacc tagcaataca gttgcactaa ataaacttcc taaaatcatt tttttcatgt
     3001 gtttttcctc ttctcttatt tattgattta tttcaaaatc atctagttcg ggaagcgtgg
     3061 aaactagata atttttttcc tgttcttcca attgaaaaga cacttttttt gtttcttgtg
     3121 tctttttctc ggtttccaat gtaatgacat acgcaatttg ccaatgcttg ccttctttct
     3181 tcatttccca ggataaaata cttttgactt tttccttttg aggactgatt tgtttcgcaa
     3241 ctttttccgt tagaaacgat tgaagctttt tcctataccg ttcttctgcc atttcacccg
     3301 aataataatt cgtaataaaa taacgactaa atgtatcaat ttgtggttgt tttgtcgcaa
     3361 gttcttccgc aaatgcaatg cgttcttgta actggtgctc ctttttcgtt agttgttcaa
     3421 tctctctttg cgttgaaagt tgcataataa aaagaccaca agatacaacg acgataaatc
     3481 caaacaaaag ccattgaaaa gacatttttt tcttctgaag agagccgttg ctttgtgtaa
     3541 acgctgtttc tacagaaagt ggtgttttct tttccttttt gatttttgtt ggtcgacgtt
     3601 cgttcttttg gtctttcttt acttgataaa acttcggtaa ttcggaattt aaggtttgaa
     3661 taaaggtttg tgcttcttca cttaattgcc cattttctgt taaagattgt tcaatcaatg
     3721 aaatcaaatg aaactcttct actggaaaat catacgagcc ttcaaatact ggaatttcat
     3781 ggttttctgt gtagatttcc aaggtacacc agccaatttc cgctcgtgcc aatagctctt
     3841 cattttctct tttcaattga ataaatgcct tatctaatgc aattttttta tctgttagta
     3901 aagacatgtt atcaacttct gtaaatcgaa tcatcatcat tagtcaaaca cttcttccac
     3961 cacttctacc gcatacacca cttgaatagg gttatttaaa gaaaatttca cccgatattc
     4021 ttgattgttc aaactgacga tatagctatc ctcatcttca gcataatagg tatattcgtt
     4081 gcctttgact tgaaaatatt tttggatcgc ttgtctttgt tgccattcgc caatctttgg
     4141 aaaataggcg aaatgatagc caaataaaac gcctatccct aataaaacaa ttcctgttac
     4201 tataatcatc tggtttcctc ctgtacctgt ttatcttcta ttgactcttt tgtcggtaag
     4261 caataaagaa ccagtaacac aagagagcca atcgttggga caaaatagaa aaaaataaat
     4321 gcccaatgaa aacctgcgtc tcgtaaacgt cgaacgctga gagaaagaga cgggacaaga
     4381 actacgaaac aatacacaat caatacgcct atcaaacaac caattattgt ttcagaagca
     4441 ttcaacgctc ctgctagtcc aatcgctaag cctaggccaa acccaatgag ggcattcatt
     4501 aacgcaacga gccaatactc ttttcttgtg gctttccctt tgtaattaaa gctattctgc
     4561 caaaattttt tataatttgt gatcatatct ttgtcctttc taatgtttat ttttgattct
     4621 agtttcttct gttttactaa caatgaaact tgccataaaa aaggtggcca atacaccacc
     4681 agctagaaat ccacttacaa ataacaaaat tttttcctcc tttctaaaaa atttcgccat
     4741 aaaaaggcaa agtatgaaca cgataaatac catgtacata ctttgccttt ttatgcaata
     4801 tatgttaact ctccaagcaa ttactgattt ttaaattctt tataaataaa ataaaaatcg
     4861 tttgagtaaa gaaataagtc aatgaagacc caaacgataa aaaatattcc taatactata
     4921 tttaataaac tacgttctag atagatacga gatttcaaat taaaaaacga aaatgagaga
     4981 tacaaatata tcaaatattt aaaaagtaac cacattttct ataccactcc ttatcagaaa
     5041 cagccttatt ctttagaata agactgtttc tgggttgatt aatttgtctt ttgttaaact
     5101 accatctaag gcaagttcta aatgtaaatg tggtcctgta ctatttcctg ttgaacccac
     5161 ataaccaata atttgtcctt gttcaactgt ttgtcctacg ttcacgcaat attcctgttg
     5221 gtgcgcataa agagccacac ttccgtcttc atgctcaatt gtgacataat ttcctgacaa
     5281 agtatgatac gaagcttcta tgaccgttcc tgccttgctc gcatagattg gttctccttg
     5341 attggctgcc atgtctaatc ctctatgaaa ttcttctcct cgccaaccga aggaactaga
     5401 aattgaataa ttttttagag gtaccatata gccttctttg cttagttgtg gaccagctac
     5461 ttctttatca tatttgatta agtcataggt ttcgattaac ccatttaatt ttttatcgta
     5521 gctagtgtct gttgcgtatc ttcctgttaa aaatttagtc gcttcctgat aggtttttgc
     5581 cttacttttc caaacaccat cataaaaaga ggtatttcca actaagcctt cttttaacag
     5641 tttggcatag tcttttaagc tttcttcata gctctcatat tgccgaaaag ttgcttgggt
     5701 cgtataaagt gtcccattgc ctaaatcttc ttgtgtcgca aaagagacgc cctttccgtt
     5761 atgtgtgcct ttgattccaa atagattgta gtttggtgcc tgagccaatt gactttgtcc
     5821 gcttgcggat tctaaaatgg cttgcgcgat cataacagaa gcgtataaat cattttcttg
     5881 tccaatttta cgagcagatt caccgatttt acggataaac gtttctacag acgcgtcttt
     5941 ttcaaaatga atagtccctt cgttatcttg tgtgctggaa gggactaatt cttgtatcgg
     6001 tgcgcttggt gcttggtggt agttttttgt ctcttctgaa ccgattgatt gtgcctgcgg
     6061 tacgtcattc ataggtacag tcacactctg ttcaggtgcc ttttgtgggc gttccgtttt
     6121 tgttggcggt tccgcttctg tttcggtact tggtttttga ggagtctgtg agctttcatc
     6181 agaaggttgc tcatttggtt cttctgacgg ttcctcctca ggttgttgat tgtcttctgg
     6241 tttgtctggt tgattcggtg tttctggttg ttcttcagaa cctgttggtg gagcgttcat
     6301 ttctggatta tctggtgtat ctattgtatc ttcagtcggt gtttctggtt gattttcttc
     6361 tccagtggaa tttgttgtcg tttcttcagt gatcgttgca gattgcgaat ttgtcagttg
     6421 attgttgcct tgttcattag ccaaaaccgc tggcgtatac ccaaatactt gacctactaa
     6481 taacaaagca gataaacatt tactcgtttt tttcacaaga ttgcctccat tcttcaatca
     6541 atcatctaga gacagctgat tgctgtctct agtcatactt taattgcttt gtttcatttt
     6601 acgacgaacc agaacagccc ccacaccaac aaaaagaaac agtcccaatg caattaacac
     6661 atcgttcact gtttctcctg tttttggtaa gctttgtttt ggtgtgctcg tcggcgtttg
     6721 tttgacaggg gttgtcggcg tttctttttt aatgcctttt gctgtcaatg tttgtttttc
     6781 attcttcaaa tcaaagtgac gtgcttcttc ctctttatgg tctttatcct tatagacaat
     6841 atgagttgcg accaatttct ccccgtcttt taacgtgcct gctttgtagt caaaggatac
     6901 tttaaactct tcagacgctt tagaaacttt acgtgcagtt tctaccgtac caacgactgt
     6961 atcttttcca tttttatcaa tcttatggaa ttgagtcacc cagtatttgg tttcccctac
     7021 cgtagattct ggaaattctt gcgatacatg atcgatcaac tcttggtctt tgtctggatc
     7081 aaatgtttgg tcgccattca ctgtaataaa gcgtgtttcg atttttggtt ctttcgcagt
     7141 cactgtttga tctttattgg ttaaatcaaa atgacgagcg aattcttctt cctcttcttt
     7201 gtcgttatag acaatgtgcg ttgcgacaat cgtgtcaccg tctgaaacca tgtctgcttt
     7261 gtagttgaac gtcacatcaa atgtttcttt ttcatcggtc acttttcgtg tttcttttac
     7321 cgtatctaat acagtgactt ttccatcggc agtaatttta tggaattgcg tcacccaata
     7381 tttctctgtc ccaaccgcag atttagggaa tacttgggac actttatcca ctaattcttg
     7441 gtcttttttc gggttgaaat cttttccacc tgtcgttgtc acaaacaaag tagaaatttc
     7501 tggttggtct tcttctggaa ccacgatttc ttcgtttttc acagtaattg gttgtacttc
     7561 tccgtctttg accgttacta aaatcgtttc tgtttgtcct gacgcttgcc cataatgctc
     7621 agggaacaca atttccgtaa atttatacgt accgtctggt aattttagtt tttctggttc
     7681 ttcttttgtt tcatacacaa aggacacatt tttattatgt gcagtcatac cttcaatcag
     7741 gaatttcgct cctggtaatt gtagatgatt gattgaatcg actttattga agtctacttc
     7801 acccgttttt aacaagttgt caatcacatc taattcaaac gtaagcacag gcgtcgtgtt
     7861 atcgccatag gccacagaga acgtggtttg ttcttcagaa ggttgacggt agttgtctgg
     7921 cacatttttt tcaatcaatt ggtatttgtt ttgatcgcca ttgtagaatt ttttgtttgc
     7981 cttccagtgt ccgtctttgt cagtttttac tgaatccaag acttctcctt ttttcacaag
     8041 gactgtttct ttatctggcg cagtaatatc tgttttggcg ataatatcaa attcgatatt
     8101 ttctgcagga acggtatcat aaacaggttt gccttgattc caggattctt ccgcattata
     8161 gacttctttg actttctttc cttctggaat gaactgttgt aagtcatttt ttgcagttaa
     8221 cgtcacttct ttgtttggtt caatcgtaat cgtatgagtt tgtttgtcta gaatataccc
     8281 agctggtgct ttgacttctt tgacatccaa tttattttga acatattttc cgtctttttc
     8341 atagaatgca gggatttctt ttaaggcgat ttcccctttg gcatccgttg tatattgttt
     8401 tgtttctccc gtatcatgac gtttgatttc atacgtcaca tttggaactt ttttccctgt
     8461 gacagcgtct agtttggtta gtttgaagtc gccatttttt agaactttaa tagttagtcc
     8521 agctctatta ggatctggca cataaagctt tcctagattt tgagcaacag gatctacata
     8581 ggcaaaactt gaacctaccc ctctagaatt tggatccagt gaagaaacag aaattcgtcc
     8641 gtcatttgag ttttgatttg cttttataaa caaactattt cctgattttc ttacttgaac
     8701 gttagctgtg ttttcaacaa ccgacccatc ccattgagaa aacacacctt ttgtatcctt
     8761 aacttctact tcttccccta ctttaaccgt cactgtttgg ctatcaaaag aaggatcttc
     8821 aagtaaattg tttatttttc tatctacagt ctgtttgaaa gcttcataat tagattgtcc
     8881 aatagttcca taattaacaa atgaaccacc taaaacttcc caaatatatg tttgtgctaa
     8941 tacatagtta tccattgaac cactagaata gtagccaaaa taaccaatta aagaaatctt
     9001 ttttctaata gcacttgtcg taattaaatt ttcgagtggt gtcacatcat attgaatacc
     9061 cgcactcgtg attgcgcctt cttgaatgca aaaaacatct tctccgtcaa tttgccagcg
     9121 aaccatgtaa tcatgagtat acattgttcc ttcaggagta attcgagtca tattcacatc
     9181 tttacgacca ttttcagtca atgttagatt ggtagcacgt gtctgtgcat tcttttccaa
     9241 gattgcttgc cattcttcag attcttgcgc caatcgttct tgttcagaaa aagaagtcgc
     9301 tttttgaata tcttcaatgg ttataaagta tttctcataa ccttcaggga cctcttgagt
     9361 cattacattg ggcggagaaa catcttctgt cgtgtcctgc gtagttgtct cttctgttgt
     9421 ttcacttgtg gtttcagtac tgatcgcttc ttcactactt tttgattctt cagtcttcat
     9481 tacttcttca gtagaatttg attcatttat cgtttctgca aatgccgtta cagatcctaa
     9541 catgggattg agaaatgtaa cgaaaattgc caaccaagtt gccattcttt taaacatatt
     9601 tttttcgttc ctttcatttt atttttcagt cttctaaagt aatttcttat ggacttcgac
     9661 accgaaacat ttttatcctc ctcaaaacaa aaaaagacta gaacttataa ttctagtctt
     9721 catcaaccac ttacttttct tttattttta gtagaaatct acagtgtgtt tttgtgaccc
     9781 gtcactgtaa ataataacat ccaccataaa acctttgttg taccactgcg aatttggatc
     9841 gctaacctct gcattagccc aatcataggc ttcatctaca gtgttaaata cctttccact
     9901 attaccaatc tctccaacta caattgttgg ctcttcaggt gtcgttgtgc caccatttcc
     9961 gccttgatcg cctccaccag gattgcttgg ttcttcaggc gttgttgttc ctccacctgt
    10021 gcttgggtta gaaggagttg tttctcctcc agtatttcca ccattacctg gttgtgtagg
    10081 tgtttgagta gtgccaccat tgttttctgg tgtacttggc gttgttgtac caccagttgt
    10141 tcctgtttga tctccagctg gtgttgttgg tgtcgttgta tttggtgtcg tttgtgtatt
    10201 tgaagtcgtt ccttgtccta ctggttgatt cggatcaact ttgtctttct cttgaatcac
    10261 tgtttgagcc acagtttcac ctgcgctgtt ttgtacactt gctacattgc cattaaatac
    10321 taatttattt cctgcataga ttaaatccac atttgtaatc ttattcatgt ccgctaattt
    10381 ttggacagta atatttgttg cttgactgat tgcgcttagg gtatctcccc agacaattgt
    10441 gtattcattc cctttaattt ctgctttgat ttgttccgcc gaacgaggtg tccaaacttt
    10501 gttttctgct gcttcagcat tcgttcccac agcaagacta actcctccga ctacgattcc
    10561 tagtgccaat actttcaaca ttttcattgt ttttcgcctc cgatttgaat aaatttattt
    10621 acaaaaacat agtaccaaac ttatgtctat cagtcgatat attttttgta gattttttta
    10681 ttttttgata tactaacttt ggtactaaaa taactaaaag gaatgtcaca catgcaactt
    10741 aataatttaa atgaaataca tgattgggct caaaataatt tacttaatcg taaacaagct
    10801 gccaaaatca ctggacaaaa ctatgatgct ttttctagct ctttacgtag aaataatatc
    10861 aaaccatttc tagaaatagg cgacgaacct ggtccttcca tcattcgctt atatctaaga
    10921 gaagatattg aaaaatacgc aaaacagctc gaagaaaaaa gacagaaacg tcaatgaggc
    10981 gttttctgtc ttggatttac aagtaaaaca ataataaaca cttttacaac ggaggttcta
    11041 tgaataagaa atacattgtt tcagttggtt caatcgtact gattgtactc gctattattg
    11101 ctttttctgt atccaacaaa acagaaacac agccacaaaa tacggttcaa tcgactacta
    11161 caagtcaatc tagcgatcat tccgtctatc cttcactaaa tagtttaaaa gaaacgcatg
    11221 gacaagattt aattgatctt ggttcttctc aaattactac tgtcttattt gaaaagaaaa
    11281 atattgcgtt tattactcct aaagaaggaa ctgaccttga attttctatc aaagtaacaa
    11341 ataccaaaga aaatttattc tccattcctg acattcaata tacccctaac aagttaaaga
    11401 ttggggatac gtttggtttg gctaaagtaa atgaacaata ctttgcttac tcactgaaat
    11461 aagtttctaa tagcaagagg aaaaaccctc ttgttatttt tatcacataa tcattttata
    11521 aaaacgcttc gaaaatatca acatttttga agaaactatt tttctaattt ctcaatttct
    11581 tccaagggat tttcgctaat gacccataca ggcacagatt gctctcgagg ctccccttct
    11641 tctaaccacc aacctgcata atgaacttct tcctcctcag cattaccatg ataccaacca
    11701 acaaaggatt catctacaac gaatgaaatt tcttcttctg gatcaaataa ttcatttttc
    11761 catgcttcaa ttgcttgttc tttggtgtac ttttgtttgt taaatacacc acaaatttcg
    11821 tcatctacat aaaaaatttc aaaatcaaaa ttactcgttc cttttactgg catcttaact
    11881 tttcctcctt tgactcactt caatagatat accactcatg ttcattccat tgtttactag
    11941 ttaattggag ttttatctta gggctttgtg cactctttcc aaaaacttga gcgtcgtttt
    12001 tttcttctaa ccaaaaaagg taatttctta cattcggtac taaatgtttt tcctgcattt
    12061 cttcatgaaa aacatataat tctgcttcat ttttcaacca attcattatt cattccctca
    12121 ttcacttctt cataccgcca ttcagatagt gagatttctt tttgtttctc cacttgtcct
    12181 ataaaaatcg ctgtattcaa caagctgatc acacttaata ataaagcaat gaaactaatg
    12241 gttacactta ctctttcaca ttttgtcatt ttgtttcctt ctttactcgt ttgttatttc
    12301 ttgtaatagg tctaataact ccttcaccga aatttgcaaa tggttcgcaa aaatttcaat
    12361 aaaagcactc gcacacatta ccagaccttc aactgatata tcactgcgac aataaaattc
    12421 tggagaaaca ctgatttctg attctttctt acaattgaac gaaaaaacta tttttgaatc
    12481 aaattccggt aattcttcca caactatgtt ttgggattct accaagaaag ggagtgcctg
    12541 ttctaatgct tcttctggtg tttgagcaga tagaataatt gcttcttgtt cttttatttc
    12601 attgttctca aatactttat aacttttttt cattgttagt tcctctttca ttcctctctc
    12661 aattagctaa gccgttactg gtacgccatt ctctaaattt agtatcgcta atccttaatg
    12721 ctcgtcgaat atcaatcatt cgataaccta ttgcacgaag ttctttatat cttttagcag
    12781 ttagcgtctc taacgtcatt tcaggcaatg ggttcgaatc aaaaatagaa ttcgaaccga
    12841 ttatttgggc caattgttgt acttgtaaca gctcttctga acttaattca gtgattgctc
    12901 gattgcctat cattgcatac attttctgtc gaatatactt cttttcttgt ttagacaaca
    12961 tttctatcca ctcctatttt cattatcatt tgttacctcc acttcagtaa atccacaaat
    13021 aaatgaattc atattccaag taccattccc ttcaactaca acttttctcc tgtcctcttt
    13081 cggatattca agaatcagac cgtttaccaa tatgtttttc actactacta atttgtttgt
    13141 ataccgtgga attgctgttc cgatatactt cactttattt cctacgctaa ttgtcatttc
    13201 ctcactccta ttctttttta tccaaaactt tttcctgtcc tattcctgcc aacgaatata
    13261 cttcactgca tgcttaaaca aaacaaaatg ttgccctgtt ttatttccgt cttttacttg
    13321 ttccacatac agttcatatt tggtcacacc ttttaaaacg ccagatactt tttttcatcc
    13381 attgtcacaa ttagtttttt catcaatcga ttgatactgc actgctcatt tttcttgttc
    13441 atatagtatt cctttctttt tcaaaactag cctcttttgt ttactagttt tcatcaatca
    13501 tcaacgattt aacagatttt ttctttcgca gggtttctag cgtcagcaac cccaaactct
    13561 aaaaaagtgc ctttgctccc ttgaaaaacg agcggacgat tgtactcgtc atcatatgtt
    13621 tttcatacta cctgatccac gcaaataacc tttttttgtt ctgtgccaca cccatccata
    13681 cgtatcgctt gagattcacc atactttttt aggtggttta tccttcctaa gtccctgtta
    13741 gacacgctga ttaacttaga aagtcctgtt tgtatccaat tttcaaagat ctcccaaaag
    13801 aaataagtag gtttacgtgt atcgcttctg ttcaaagtac ctgtttgtac ttgagtattt
    13861 ctactcccct cgttcaaggg attccttcca ccagctagct tttcctttca tcatcaaatg
    13921 ttcctgaaaa gcatttacgt ttcgtgtccg tgtgcttact tattcttttt tcgatagcga
    13981 ttatccgctc gctactcgct attttatact ttgtttgtta gctcaaataa aaactaacaa
    14041 acaaaaaagg ctagtttctt tccgttttat ttttttaact gtcgataaat agttgttagt
    14101 ttaattccaa gttctaaaac caatacgccc aacaaaacca caggaaaaac aattggggca
    14161 aacaagtaaa atcggatagt tttcatgtct tccctctttc tttttggtgt cgctgtggtc
    14221 gttcttgccg ttcaatactt cgatacattt tttattcatt atttgctcgt tttgttttcc
    14281 tctggactgc ttttcttgct ttttgaatac tttatacgct tttcgtgcct gaattaatag
    14341 gtcttgtcgg cgtttcattc gtgagaaaac aatatttgaa accatcaaat cctggttcga
    14401 acaaccacga ttcgcaataa ttttcccatc aaaaaaaata gttttcactt gagaaaaaaa
    14461 cagttggaaa tcttgttttg ttgctaattt ttttgtcaaa atcactgtgc ttttccctgt
    14521 tgaatctaca atgattcttc gaacaatccg attcgataac ttccaacgtt gccgattaaa
    14581 caatgttact tcacaacaaa tatgacgtaa gtgattaggg gtcattttca tgtgtccaat
    14641 tcctccaacc aattatgcat cgaaattttg ggcaattgtg acagcccata aatctgcgtt
    14701 tccaggtctt cataactaca agaacgcttg gcttgaagta atgaatattc tgcttgatct
    14761 gatagttttg ttgccattgg atctagattc aattctctta cacaacattc ccaaaaattc
    14821 aatagcgctc taaaataaaa tccttttcga ttctgaattg cttttccttc atgacgagca
    14881 attttttcat tttgaataca ccgttgatac gttttgctta aatcttctgc aaatgtttcc
    14941 cctaaaattc gatactcgct tccatcagat aaaccatgtg tttgattata ctttcgttgc
    15001 cgcttttcta taattcgttt actgtcgtat atcatgttta gctgctcttt cgcttcacgc
    15061 cattcagctg aactgcgaac aatccatgtc aaaacatctt tcggaacaaa cgtactttcg
    15121 ctgtaatgag caataaactc tttttgccaa tcatcagcca tttcaacagg actagtcgta
    15181 tcgatttttg tatctagatt ttctgtatct agattgttag aagaattatt aaataattgt
    15241 gcagaatttt cacatggatt tttttcgtca aaatcatcga aaatcgctaa attatcaaca
    15301 gattcctcct cttcttttcc ttgtgcagaa tctacactgg ttgagtcaac aggacctaat
    15361 gaatacacat cattttctgt cgtatctaat tctcctagat acaaccgagc tttgtttctt
    15421 tttcctgttt gttgatcaaa tcccatatcc tctatttcta gtagctcata ttctttcaat
    15481 tcttttttga ttgtattaac agaacgtctg caacaattca atgcattggc taattcttgt
    15541 tccgtaaatt caaaataaac aaaaccttga ctatctactt gatttcgttg aatggctaaa
    15601 tctaagcgtg accgaaaaac catatacgcg atttttgctt tatcagacaa tgagcgatat
    15661 ttttcacttg taataaatac ctttggaaat tgatagtata attcattgta tttacgagtt
    15721 gcggtaaatc tttttgttgc catcgttatt acacctcatc ttgtccatat attaagaaag
    15781 aattttggtg ttggtagaca cgaacatctt ccattgtagc aatcaattct tttagataaa
    15841 ttcgaccgaa ttcaattgtt agtaagtcgt aatcttctaa ttcatttttg atttcttcaa
    15901 tttcttcttc agacacattg agtaaaatgg ctaattcttg attggtataa gaaatataga
    15961 catgtttctt ttcatcaatc cgtttttcaa ttaaggattc ttctaagtac tcattcagta
    16021 agctataggc gtattttgct tgtgcagtca tcgcataata ttttgacgat tttataaaga
    16081 atagttttgg catatgaaaa gttgcttgtg tcagcacatc tgttgcgcga aatttgttt
//
LOCUS       DARBET010000057        16095 bp    DNA     linear   BCT 07-NOV-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4027
            SAMEA114569841-rid20112183.denovo.059, whole genome shotgun
            sequence.
ACCESSION   DARBET010000057 DARBET010000000
VERSION     DARBET010000057.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA114569841
            Sequence Read Archive: ERR12203666
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 16095)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16095)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001972618.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001972618.1
            Genome Coverage       :: 132x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/07/2023 13:18:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,041
            CDSs (total)                      :: 2,975
            Genes (coding)                    :: 2,908
            CDSs (with protein)               :: 2,908
            Genes (RNA)                       :: 66
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 67
            CDSs (without protein)            :: 67
            Pseudo Genes (ambiguous residues) :: 0 of 67
            Pseudo Genes (frameshifted)       :: 26 of 67
            Pseudo Genes (incomplete)         :: 28 of 67
            Pseudo Genes (internal stop)      :: 20 of 67
            Pseudo Genes (multiple problems)  :: 6 of 67
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16095
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA114569841-rid20112183.denovo.059"
                     /isolate="VRE4027"
                     /db_xref="taxon:1352"
                     /country="Denmark"
     gene            226..894
                     /locus_tag="SCJ86_002038"
     CDS             226..894
                     /locus_tag="SCJ86_002038"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286865.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF421 domain-containing protein"
                     /protein_id="HEG1169571.1"
                     /translation="MIFSITMKLVFGLIGLLLVVRLLGKKSMSEITPFDLVYTLVLGG
                     ILEESTYDDNVHVGHVLFAIALWAVMIYGIERIVQKNEKVNRWVKGEPSVLIKDGVIN
                     MTELTNNHIEMEQLRAILRQQECFSLENAKHVILENAGQMSVLKKSDEDKALSILLVD
                     EGQIQHKVLQSNQLTEAWLMENLKKEGYADVKQLIYVEWSEEKGLYILNKEDVMHESY
                     RIDG"
     gene            complement(1094..1480)
                     /locus_tag="SCJ86_002039"
     CDS             complement(1094..1480)
                     /locus_tag="SCJ86_002039"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342909.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEG1169572.1"
                     /translation="MRRTAKKFIDYNEYRDRPFGLKWGTAFAMEELVNGIKKNEEEAR
                     KQPLPKQQMTLQEIDSILTEAFLSRKAVIIQLNLKDEFGRLLDEVEGHFIGEAYEDYF
                     MIDHQPILWEDVRYIELKETKKWFVT"
     gene            complement(1452..2834)
                     /locus_tag="SCJ86_002040"
     CDS             complement(1452..2834)
                     /locus_tag="SCJ86_002040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286860.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Y-family DNA polymerase"
                     /protein_id="HEG1169573.1"
                     /translation="MWANQNPVFVYEKEPSRDILCIDCKSFYASVECVEKDLDPLTTK
                     LVVMSYPSDSTETRGSGLILASSPAAKKAYGITNISRARDLPFPYPSDLYIVAPRMAY
                     YMEKNREINSIYKKYADEANHHVYSVDESFLDVTNSMALFGKTNAYDMAKLIHKDVFE
                     QTGIYTTIGIGDNPLLAKLALDNGSKDGKQMFAEWRYEDVPEKLWKISDITDFWGIGK
                     RTAKRLKQMGIHSVYDLAHADYYHLKSKLGVIGTQLYAHAWGIDRSFLGQEYTPKSKS
                     IGNSQILDRDYTRREEIEIVIKEMADQVGTRLRRSAAKTQVVSLWVGYSLGYLDPNGK
                     TGFHQQMKIEATNASRDLADALLTIFHQHYHYQDIRRVGVNCSDLVYATGLQLNLFED
                     PEKQINEAKVDFVVDTIRKKYGFQSIIHAHSLLQGGRAIARSSLVGGHAGGMSGLEGG
                     KDETDSKKIH"
     gene            complement(2990..4177)
                     /locus_tag="SCJ86_002041"
     CDS             complement(2990..4177)
                     /locus_tag="SCJ86_002041"
                     /EC_number="2.7.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314506.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactokinase"
                     /protein_id="HEG1169574.1"
                     /translation="MEIKRSLNTKFAETFGPKKTTQYFSPGRINLIGEHTDYNGGHVF
                     PASITYGTQGVAAPRDDNKILVYSTNFDDIGVISFTLDELEYDKKANWANYVKGMVLK
                     LKEAGHSIDHGFELLVEGTIPNGAGLSSSASLELLVGVVLEDLFDLKTDRLELVQTGK
                     KVENEFIGVNSGIMDQFAIGFGELDHAILLDTNTLKYEMVPVKLDGYAIVIMNTNKRR
                     ELADSKYNERRSECEEALARLQKTLPISSLGDLDEEEFESTIDQIGDDTLIRRARHAV
                     SENQRTLKAKAELEAGNLEAFGQLLNDSHHSLRYDYEVTGIELDTLVDAAQKQEGVLG
                     ARMTGAGFGGCAIALVKEENIPAFKNNVYDEYMKVVGYAPEFYVAHIGNGTTKIDPEA
                     VHV"
     gene            complement(4184..5215)
                     /locus_tag="SCJ86_002042"
     CDS             complement(4184..5215)
                     /locus_tag="SCJ86_002042"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346498.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactose mutarotase"
                     /protein_id="HEG1169575.1"
                     /translation="MKITEKDFGQGYHLITLENKNKLALSISDLGARIVSLKSNDREL
                     VLGFDTAEEYIEKDPYIGASIGRTAGRIENGRFSLNGKTYQLATDPKTGHNLHGGAPG
                     FELKKWSYVILNGENEASVIFTTTSPDGEHGFPGTMDVEIRYTLTKDNIWRVTSRGTS
                     DQDTLFNPTNHVYFNLTGDASQSIDQHELWLNSEAYAPLRTDSIPIGVKENAAGSAFD
                     FQIPKKLASVFASDLDQKNLVDGIDHPFFLKETGLGKEAARLTSPDKRIQVGIATDAS
                     SVVIFTANFGTETPEMRNRKLAHHGGITFETQTAPGAERFSAFGSIHLKAGSVFETVT
                     EFKIKTRKE"
     gene            complement(5231..6685)
                     /locus_tag="SCJ86_002043"
     CDS             complement(5231..6685)
                     /locus_tag="SCJ86_002043"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336053.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEG1169576.1"
                     /translation="MKSKMIQRVSYALGAFGHDTFYVTLSTYFMVFVTSQMFAGTDKA
                     TNAKMIATVTSLVVGIRLVEIIFDPLIGGIIDNTRTKYGKFKPWLVIGGVVSSVLLVA
                     IFTNFFGLATHNTTLFIILFAIVFILLDSFYSFKDIAFWSMIPALSTETKEREKFATF
                     ARFGSSLGANGTTLVVVPIVTFFTYLVTHKHIEGASGWFWFGVIAAVISGGTAIITAL
                     GTKEVDTAIRQQNEKAGIKDVFKAVAQNDQLMWLALAYIAYAIANVATTAVLFYFFKY
                     VLGQPTEFWIVGLVAALLGLVAVPLFPMLTRVISRRYVYLGGIAMMISSYFFFTIAGS
                     SLPIVIIGLVLFYFPQQLIFLSALMTITDSVEYGQWKNGIRNEAVTLSIRPLLDKIAG
                     ALSNGIVGFVAIASGMTGNATAADITAHGVTTFKAYAFYAPAALMIFSAIIFAWKITL
                     TEKKHAQIVEELENKLAPADTMEDELHDAVVPSH"
     gene            6860..7765
                     /locus_tag="SCJ86_002044"
     CDS             6860..7765
                     /locus_tag="SCJ86_002044"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348792.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
                     /protein_id="HEG1169577.1"
                     /translation="MEQSIFLKKEHQQVEKELYFDFCGFSKTMPFHSFGPAIRNDYIL
                     HVVMEGKGSYHVKNQQFQLKKGDLFLIRPGDSTFYLSDGEDPWMYCWLSFGGSAAEEI
                     IRQSLFREDQYTMVSSDIQKYIDIILECMKDTEETLENELQLTALTYRFLSVLLKDGG
                     KVSGGVQKVHSSLAVETVKYIEAHYAEKLSVEEIAQHLAVNRSHLSRVFKNHMGTSIK
                     EYLIGVRINRGAFLLSLTDESVESIAYQVGFNSLVVFSRMFKKSTGETATNYRKRMKN
                     ENFKNIALDTLKKQLEKQTAVSWTT"
     gene            7856..8533
                     /locus_tag="SCJ86_002045"
     CDS             7856..8533
                     /locus_tag="SCJ86_002045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314509.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent oxidoreductase"
                     /protein_id="HEG1169578.1"
                     /translation="MRTLIIISHPTIEESLNQQFFKEASAGLPATWHHLESSYPDGKI
                     DIETELQLLAQHDRIIFQFPFYWYSSPAHLKIWQDNVLERAAHVLKGKELGIVLTTGV
                     AEKEYQAGGKEEYTISEFLRPYQRIANKFHMTYLPPFVLAQFMYLSQEKRWEKLIAYQ
                     QYLSLEGKPSLTQRIDWFIQRVQENQKMQEEESEKQAYIVEALTEAKEQIEDLTFTLQ
                     EMKGTSL"
     gene            8530..8700
                     /locus_tag="SCJ86_002046"
     CDS             8530..8700
                     /locus_tag="SCJ86_002046"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291423.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEG1169579.1"
                     /translation="MTNDWLIQQLDELSEKSEYQDRALLYEIKKLMAEIDERTMQLQG
                     EIDGQSWNHLNW"
     gene            8814..9710
                     /locus_tag="SCJ86_002047"
     CDS             8814..9710
                     /locus_tag="SCJ86_002047"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314511.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysM peptidoglycan-binding domain-containing
                     protein"
                     /protein_id="HEG1169580.1"
                     /translation="MYDENEWGENVRMTRRRQQKQAARKRMIMIGAAVAVFLIIAGGV
                     AGLYFHGKNSSEAKSTTITETTTKKTETKQTSESSTKTKETQTSEEKTANGLPEQLQA
                     IVNDYSKKIEEKTPALIEEYQTEIQGNQEGIAGLSAVANQKARELQAISDEGIRKLRA
                     AYQAAENKDGVDLDTLINQLSANYTNHVAKISDIYLQTSASLQAESTSTQDTTSSDSE
                     TAESVEASEMQARTTQDTTDSSETNTNSATSQAATTVVLEGEGPNQIAERTGVPVETI
                     LSLNGMSMENYFLTPGETLKLN"
     gene            complement(9765..11135)
                     /locus_tag="SCJ86_002048"
     CDS             complement(9765..11135)
                     /locus_tag="SCJ86_002048"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297903.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HEG1169581.1"
                     /translation="MEEEKLTRSLSARHIQMIALGGTIGVGLFMGASSTIRWTGPSVM
                     GAYAIAGFFLYLIMRALGEMLYVDPSTGSFANYASEYIHPVAGYLTAWSNIFQYIVVG
                     ISEVIAVGSYMNYWWPDLPAIIPGIIVVLFLMLANLISVKAFGELEFWFSIIKVITII
                     LMIIAGLGVILFGFGNHGQAVGISNLWKNGGFFTGGVKGFFFALSIVVASYQGIELIG
                     MTAGEAENPKKTIIEAVQSTIGRILIFYIGAIFIIVSIYPWNQLSEIGSPFVQTFSKV
                     GITFAAGLINFVVITAALSGCNSGIFSASRMVYTLAINGKMSKKFLKLSRHGAPFYPV
                     VAISGGILIGLILNYLLPMFINQSENIFVFVYSSSILPGMVPWVVILISEIRFKKKHA
                     DKMHDHPFKMPLSPYTNYVTLAFLAIVLIFMFINPETRISLIIGLVFLLYMTIHYFVR
                     EKRTGK"
     gene            11373..12119
                     /locus_tag="SCJ86_002049"
     CDS             11373..12119
                     /locus_tag="SCJ86_002049"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter ATP-binding protein"
                     /protein_id="HEG1169582.1"
                     /translation="MERTAIVIQDLSAAYQGKTVLKTINLTINPQKITGIIGPNGAGK
                     STFMKSLLELIPATTGKVTFSGQPVNSVRKKIAYVEQRSELDLSFPIDVLGVVLLGTY
                     PSLRIGQRPGKKEKERARQALKKVGMEEYAKRQISELSGGQLQRVFIARALAQGAEWI
                     FLDEPFVGIDALSERKIFDILQELKNSGKTILIVHHDLHKVEEYFDEVILLNKQLIAS
                     GPVQEVFTSKNLQLAYGEIIRHLVKGGEKK"
     gene            12116..12955
                     /locus_tag="SCJ86_002050"
     CDS             12116..12955
                     /locus_tag="SCJ86_002050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter permease"
                     /protein_id="HEG1169583.1"
                     /translation="MIQSFIDGLMNYQFLQYALVTSMLVGLASGVIGSFIILRGMSLM
                     GDAISHAVLPGVAISYMFGFSYIFGATAFGMLTAAVIGFVTQRSRLKNDTAIGIVFSS
                     FFALGIILISYAQSATDLYHILFGNVLAVRESDLLLTALVSGIVLIFVFFFYKELKIT
                     SFDPTMAKAYGLNTSLIHYLLMFFLTLVAVVSLQTVGTILVIAMLITPAATAYLLTNH
                     LLKMIITAAGIGMLSAVVGVFFSYSYNWPSGATIVLACTAFFILAFLFSPTKGILFQK
                     GSR"
     gene            12956..13906
                     /locus_tag="SCJ86_002051"
     CDS             12956..13906
                     /locus_tag="SCJ86_002051"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286843.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter substrate-binding protein"
                     /protein_id="HEG1169584.1"
                     /translation="MKTKKSLFLILAVSFLVLAGCGKQASDQADEGSKEKLSVVATNS
                     ILADMAKEVGTDQIDIHSIVPVGTDPHEYEVLPEDIKKASDADVILYNGLNLETGNSW
                     FDNLMETAKKEEGKDYFAVSKNVEPLYLTSGEEHTKADPHAWLDLSNGIKYVEEIARI
                     FSEKDAENATLYKKNAEAYVEKLKELDTQAKESFASIEENKKLLVTSEGAFKYFSRAY
                     DLPAAYIWEINTESQGTPDQMKAIIDQIRAKEVPVLFVETSVDSRSMERVAKETGLKI
                     YDKLFTDSIAKEGEQGDSYYQMMKWNIETIHEGLSQTKES"
     gene            14047..14799
                     /locus_tag="SCJ86_002052"
     CDS             14047..14799
                     /locus_tag="SCJ86_002052"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286840.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEG1169585.1"
                     /translation="MNFTRKDKYTIKEEVFRLISQQENTDKDQDLQFLEAELAKLFIA
                     NQNETTEPDEELYIGKWLFDEFQPLDENTGIGYFVFLSPVGFTPGVLVKQNDEITVKA
                     WPALFSVIKAEGVIKKIQSAYYKSQFIANSKLMNMFYNRLVEIARPGKIPQTFTYTQQ
                     KNLYEEVLYSRDLSEFSPWEEVRLHELLSTKPVSLNFFNALDNKRKLISTNYWEVFAA
                     RKKWMEEKKIADQQIQFVQLKKEKLYYGLYSR"
     gene            14818..15969
                     /gene="queG"
                     /locus_tag="SCJ86_002053"
     CDS             14818..15969
                     /gene="queG"
                     /locus_tag="SCJ86_002053"
                     /EC_number="1.17.99.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA epoxyqueuosine(34) reductase QueG"
                     /protein_id="HEG1169586.1"
                     /translation="MTLKEEIIQESKRLGIDKIGFTTAEPFDSLKESLEEQKAAGHTS
                     GFEHPNIDERLYPEMTFDQPKSIISIALAYPTRIHEEVPRDEKRGQFARASWGIDYHD
                     ILRDRLNRLIEFIQTRAEKWQKEEEWRLAPQVDTGELIDVAVAQRAGLGFIGRNGLLI
                     TEEFGSFVYLGEIVTNIVFEPDKPGEFGCGDCMRCVTACPTKALLGDGRMNAQRCLSY
                     QTQTKGMMPEEYRKKMRNVIYGCDICQLVCPYNQGKDFHFHEEMEPKVDEVYPKLKPM
                     LSMSNKDFKQQFGHLAGSWRGKKPLQRNALIALANLGDRTALPEISECLTDVRPVIRG
                     TAAWAIGRLGTKEPEKWLDVLDEILAKETEEVVINELEHAQKLLRRKLK"
BASE COUNT         5220 a         3009 c         3232 g         4634 t
ORIGIN      
        1 cgtccaattt cgtaaaacaa ccgtaacggt tgtgtaaaaa tatcatttta gagcataaaa
       61 aaaaggagtg gatttctcca ctccaaaaaa gtctaacttt tgggggtcac tacagtgtca
      121 cagtcctttt tttagtaaaa ggatacattt cttccttgta tattccatgc gtgttcgtga
      181 gaaagcatat ataatttaat taacagatag ttagggggag aaaaaatgat tttttcaatt
      241 acaatgaaac tggtgttcgg gttgatcggt ttattattag tggtacgttt gcttgggaag
      301 aaatccatgt cagaaatcac accgtttgat ttagtctata cgcttgtttt aggtgggatc
      361 ttagaagaat caacctatga tgacaatgtc catgtagggc atgtcctatt tgccattgcg
      421 ctttgggcag taatgattta tggtatcgaa cgtatcgtcc aaaaaaatga aaaggtcaat
      481 cgttgggtca aaggcgagcc atctgttcta atcaaagacg gcgtcatcaa catgacagag
      541 ttgacaaata accatatcga gatggagcag ttgcgagcaa tcttaagaca gcaagaatgc
      601 ttttctcttg aaaatgccaa acatgttatc ctagagaatg ctggtcaaat gagtgtattg
      661 aaaaaatcag atgaagacaa agctttatcg atcctgcttg tagatgaagg gcaaattcag
      721 cataaagtgc ttcagtccaa tcaattgact gaagcatggc ttatggagaa cttgaagaaa
      781 gaaggctatg cagatgtaaa acagctgatt tatgttgaat ggtcagaaga aaaagggctc
      841 tacatactaa ataaagaaga cgtcatgcat gaatcttacc gaatagatgg atagatagac
      901 agaggttgtg aaatcagcgc cttggcttgt tactcaaaac taagcgactt tttcacaacc
      961 tctgatatct aatatacgtt gttccagaag ttgcttcttc gaaaaataag ccaaaattcc
     1021 acaaaaattt aagaagcaat tttcgaaaaa tcctttctta ttttttgaag ttgatcactt
     1081 ctgtcacaac ctctcatgtc acaaaccatt tttttgtttc ctttaactca atataccgta
     1141 cgtcttccca caatatcggc tgatggtcaa tcataaagta atcttcatag gcttcaccaa
     1201 tgaagtgccc ttcgacctca tccaagagtc ttccaaattc atcttttaaa ttgagctgaa
     1261 tgattactgc tttacgagat aaaaaggctt ctgtcagtat actatcgatt tcttgcaatg
     1321 tcatttgttg tttaggaaga ggctgtttgc gtgcttcttc ttcatttttt ttgataccgt
     1381 tgactagttc ttccatcgca aaagcagtcc cccattttaa tccaaatggc cgatctcgat
     1441 actcgttata gtcaatgaat ttttttgctg tccgtctcat cttttccacc ttctaatcca
     1501 ctcataccac ctgcatgccc gccaacgaga gaactacgtg cgatggcgcg tcctccttgc
     1561 aagagactat gcgcatggat gattgattga aagccgtatt tttttctaat cgtatcgaca
     1621 acaaaatcaa ctttcgcttc atttatctgc ttttcaggat cttcaaaaag gttaagctgt
     1681 aaacctgtcg cgtatacgag gtcggaacag ttcacgccta cacgccgaat atcctggtag
     1741 tggtaatgct ggtggaaaat ggtcaataaa gcatctgcta gatctctgct agcatttgtc
     1801 gcttcgattt tcatttgctg gtgaaacccg gtcttgccat ttggatcaag atagcctaaa
     1861 gagtatccta cccataagct gactacttgt gtttttgctg cagatcgtct caagcgagtg
     1921 ccgacttggt cggccatttc tttgataacg atctcgattt cttctcgtct tgtataatct
     1981 ctatctagga tttgactatt tccgatactt tttgattttg gtgtatattc ttgtcccaaa
     2041 aaacttcgat cgatgcccca agcatgggca taaagctgag taccgatgac ccctagcttg
     2101 cttttcaagt gataataatc cgcgtgagct aaatcataaa cagaatgtat acccatttgt
     2161 ttcagacgtt ttgctgttcg tttgccgatt ccccaaaagt cagtgatatc tgagattttc
     2221 cataactttt ctggtacatc ttcataacgc cattcagcaa acatttgctt tccgtctttt
     2281 gagccattgt ctaaggctag tttagcaagt agcgggttat cgccaattcc tatagtcgtg
     2341 tagattccgg tctgctcaaa cacatcttta tggatcagtt tagccatgtc gtacgcattt
     2401 gttttaccaa agagtgccat gctatttgtt acatcaagga agctttcatc gacggaatag
     2461 acatgatggt tcgcttcgtc tgcatatttt ttatagatag aattgatttc tctgttcttt
     2521 tccatataat aggccatcct tggggcaaca atatataaat cacttggata ggggaaggga
     2581 agatctctgg cacggctgat attggtgatg ccgtaagctt tttttgctgc aggactggaa
     2641 gccaagatta atccgctacc ccgtgtttct gtcgaatcgg acggatagga catgactact
     2701 aatttagttg taaggggatc aagatccttt tcaacgcact ctacggaggc gtaaaaagat
     2761 ttgcagtcaa tacataaaat atctcttgaa ggttcttttt cataaacaaa taccggattt
     2821 tgattcgccc acataacaat cacctcttca acacaattag aacacacgtt ctctttccgt
     2881 gtcaataaaa aacagcaatt ttcaaatacg agcaaaaggc tcacatttgg aaattgctga
     2941 gaggttattt ttatctattt gtttttagct gagatcgttg atttttttat caaacatgaa
     3001 cagcttctgg atcgatcttt gttgtcccat ttccaatgtg tgcaacataa aattccggag
     3061 catagccgac aactttcatg tattcatcat agacattgtt tttgaaggca ggtatatttt
     3121 cttctttgac aagagcaatc gcacagccac caaatccagc tcctgtcatt cgtgcaccta
     3181 agacaccttc ttgtttttga gcagcatcta ctagtgtatc taattcgatc ccagttactt
     3241 cataatcata gcgaagagag tggtgagaat catttaacag ttgaccaaat gcttctaagt
     3301 tcccagcttc taattcagct tttgccttca aagtccgctg attttcagaa acggcatggc
     3361 gagctctgcg tatcaatgta tcatcgccga tttggtcaat agttgattca aattcttctt
     3421 catctaagtc tcctaaagaa gagataggca aagttttttg caaacgtgcc aaggcttctt
     3481 cgcattcgct acggcgttca ttgtattttg aatctgccaa ttcccgccgc ttattcgtat
     3541 tcatgatgac gattgcataa ccatctagtt tcactggaac catttcgtat ttcagcgtat
     3601 ttgtatctaa taagattgca tgatctagct caccgaagcc gatggcaaac tgatccatga
     3661 tacctgaatt gacaccgatg aattcatttt ctactttttt acctgtttgg actagttcca
     3721 agcgatctgt ttttaaatca aacagatctt ctaatacaac acctactaat aattctaatg
     3781 aagcgctact ggaaagtcct gcaccattag ggattgttcc ttccactaac aactcaaacc
     3841 catgatcgat tgagtgtcca gcttctttta atttaaggac cattcctttt acgtaatttg
     3901 cccagtttgc ttttttatca tattctagct catctaaagt aaaactaatg acaccgatat
     3961 cgtcaaagtt tgtggaataa actaatattt tattatcgtc tcgaggtgct gctacacctt
     4021 gcgttccata agtgatggaa gcggggaaga catggcctcc attgtaatct gtgtgttcac
     4081 caatcaaatt gatgcgtcct ggtgagaagt attgtgtagt ttttttaggt ccgaatgttt
     4141 cggcaaattt tgtgtttaag cttcttttga tttccatgat gagttattcc tttcttgttt
     4201 tgattttgaa ttctgtcact gtttcaaata ctgatccagc ttttaaatga atcgaaccaa
     4261 atgctgagaa gcgctctgca cctggagctg tttgagtctc aaatgtgatg ccaccgtgat
     4321 gtgccagttt tcggttgcgc atttctggtg tttctgtgcc gaaatttgca gtgaaaataa
     4381 ccacactaga agcatctgta gcaataccta cttggattct tttatcgggg ctagtcaagc
     4441 gagcggcttc tttacccaag ccggtttctt ttaagaaaaa tggatgatcg attccgtcaa
     4501 caagattttt ttgatccaaa tcactagcaa aaacggacgc taatttttta ggtatttgaa
     4561 aatcgaaagc acttcctgca gcattttctt ttacccctat aggaatacta tctgttcgca
     4621 aaggagcata agcttcgcta ttgagccaaa gctcatgttg atcgattgac tgagaagcat
     4681 ctcccgtcaa gttgaagtaa acatgatttg tcggattgaa taaagtatct tggtcactgg
     4741 tgccgcgaga ggtcactcgc cagatattat cttttgtcaa agtatagcgg atttccacat
     4801 ccattgtacc tggaaaacca tgttctccgt cgggacttgt agtagtaaaa atgacagaag
     4861 cttcattttc gccattcaaa atgacataag accatttttt taattcaaat ccaggagcac
     4921 caccatgcag attatgtcca gtttttggat ctgtggctaa ttggtaagtt tttccattga
     4981 gagaaaagcg gccattttcg attctccctg cggtgcgacc gatagaagca ccgatatacg
     5041 gatctttttc gatatattct tcagcggtgt caaatcccaa aactaattct cgatcatttg
     5101 attttaaact gacgattcta gcacctaaat cagagataga aagtgctagc ttgtttttgt
     5161 tctccaaggt tatcaagtga taaccttggc caaaatcttt ttctgtaatc ttcattttca
     5221 agatcctttc ttaatgggaa ggaactactg catcgtgaag ttcatcttcc attgtatcag
     5281 ctggagccaa tttgttttcc aattcctcaa cgatttgtgc atgttttttc tctgtcaatg
     5341 taatcttcca agcaaaaata atagcggaga aaatcatcaa agcagcagga gcatagaatg
     5401 cataagcttt gaaagtcgtt acgccatgag cagtgatatc agcagcagta gcattaccag
     5461 tcatcccaga agcgatagcg acaaacccta cgatcccgtt agacaaagca ccagcgattt
     5521 tatctagtaa agggcgaatt gatagagtaa cagcttcgtt acggatacca tttttccatt
     5581 gaccatattc aacagagtct gtgatcgtca tcaaagcgga taagaaaatc aattgttgag
     5641 ggaagtagaa caatactagc ccgatgataa cgattggcaa acttgaacct gcgatcgtaa
     5701 agaaaaagta agaagaaatc atcattgcga tccctccaag gtaaacgtag cgacgagaga
     5761 tgacgcgtgt aagcattggg aatagtggaa cagctactaa accaagtaag gcagcaacta
     5821 gaccaacgat ccaaaattca gttggttggc caagtacata tttgaagaag tagaataata
     5881 cggcagttgt tgctacattg gcaatcgcat aagcgatata agcaagggca agccacatca
     5941 actgatcatt ttgagcaact gctttgaaca catctttgat cccagctttt tcattttgct
     6001 gacggatcgc agtatcgact tcttttgtgc ccaaggcagt gataatcgca gtaccgcctg
     6061 aaataacagc agcaatcaca ccgaaccaga accagcctga agcgccttca atatgtttat
     6121 gagtaacaag ataggtaaag aaagtaacga tagggacaac gactagagta gtaccatttg
     6181 cacctagaga agagccgaaa cgagcaaacg tagcaaattt ttcacgttcc tttgtttccg
     6241 tactcaaagc tggaatcatt gaccaaaaag cgatatcctt aaatgaataa aaactatcca
     6301 ataagatgaa aacgattgca aataatatga taaataaagt cgtattatga gtagctaagc
     6361 caaagaaatt ggtaaagatt gctaccaaga gtacggaact aacaacgccg ccgataacta
     6421 accatggttt gaatttaccg tattttgtac gagtattatc gatgatgcca ccaatcaaag
     6481 gatcaaaaat aatttcaact aagcggatac ctacaactaa gcttgtaact gtagcaatca
     6541 tttttgcatt cgttgcctta tctgttcctg cgaacatctg gcttgtcacg aataccataa
     6601 aataagtgct taatgtaaca tagaatgtgt cgtgtccaaa agcgcctaaa gcgtaggaca
     6661 cacgttggat cattttgctt ttcataatct gtattcctct cctgttcaat atataatact
     6721 aagtaaaccg gttacaacga ataacaaaga tcttttaatg acttaaactt aacaagaaat
     6781 cgcttccttt ttaatatatt tctgtgactt ttatttaact ttttgttgca ttatactaaa
     6841 ggagtaagga gggatagacg tggagcaatc gattttttta aaaaaggaac atcaacaagt
     6901 agaaaaagaa ttgtattttg atttttgtgg tttctctaaa acgatgccgt ttcattcttt
     6961 tggtcctgct attcgcaatg attacatcct gcatgtcgtg atggaaggca aaggatctta
     7021 tcatgtgaaa aatcaacaat tccaattgaa aaaaggtgat ctgttcttga ttcgtccagg
     7081 agactcgacg ttttacttat cagatggaga agatccgtgg atgtattgct ggctttcatt
     7141 tggtgggagt gctgcagaag aaatcattcg gcagtcgctt ttcagagaag accaatacac
     7201 gatggtctct tctgacatac aaaaatatat cgacattatt ttagaatgca tgaaagatac
     7261 ggaagaaacc ttggaaaatg aattacagtt aacagcatta acctaccgtt ttttatcagt
     7321 ccttttaaaa gatggcggaa aggtcagcgg tggtgtacaa aaggttcatt cctctttagc
     7381 tgttgaaaca gtcaaataca tcgaggcaca ttatgctgaa aaattgtctg ttgaggaaat
     7441 cgcccagcat ttagcggtca atcggagcca tttgtcgcga gtattcaaaa atcatatggg
     7501 taccagtatc aaggaatatt tgatcggggt acggatcaat cgaggggcgt ttcttctttc
     7561 tctgacggat gaatccgtgg aaagtattgc ctatcaagta gggttcaata gtctagtcgt
     7621 atttagccga atgttcaaaa agagtaccgg agaaacggct acgaactatc gtaaacgtat
     7681 gaaaaacgaa aattttaaga atattgcttt ggataccttg aaaaaacagt tggaaaaaca
     7741 aaccgcagtt tcttggacaa cctgattagt ctggttaaaa tgacaagcat cggcaaaaga
     7801 aacttgtggc ggttatgcta cactgtaaag cgaagactaa aaaggaggaa aagggatgcg
     7861 tacacttatc atcattagcc atccaacgat cgaagaatct ttaaatcagc aatttttcaa
     7921 agaagcttct gctggtttac cagctacttg gcatcatttg gaatcaagtt atccagacgg
     7981 aaaaatagat atagagactg aactacagct cttggcccag catgatcgga tcattttcca
     8041 gtttccgttt tactggtata gttctcctgc tcatttaaaa atttggcaag ataatgtctt
     8101 ggaacgagca gcacatgtat taaaagggaa agaactagga atcgtattga caacaggtgt
     8161 ggcggaaaaa gaataccaag ctggaggaaa agaagaatat acgatttccg aatttttgag
     8221 gccgtatcag cgaatcgcaa ataaattcca tatgacctat cttccgccat tcgttttagc
     8281 acaatttatg tatttatccc aggaaaaacg ttgggagaag ctaattgctt atcagcagta
     8341 tctctcattg gaaggcaaac catcgctgac tcagcggatt gactggttta ttcaaagagt
     8401 acaggaaaat cagaaaatgc aggaagaaga atctgaaaaa caagcatata tcgtagaagc
     8461 actgactgag gcaaaagagc agatcgaaga cttgaccttt acactgcaag aaatgaaagg
     8521 aacttcatta tgacaaatga ttggctgatc caacaattag atgaattaag cgaaaaatca
     8581 gaatatcaag accgtgcgct cttatatgaa ataaagaaac tgatggctga gatagacgaa
     8641 cgaacaatgc agctgcaagg agaaatcgac ggacaatctt ggaatcatct caactggtga
     8701 aattttctta taaataacaa agataaaaat gtgcacagcg acaattattt gctacaataa
     8761 gaatatgaag cgtttgataa cgctattata cataaaaatt ggtggtgaaa aaaatgtatg
     8821 atgaaaatga gtggggagaa aatgttcgga tgacccgaag aagacagcaa aaacaggcag
     8881 ctcgtaagcg gatgatcatg attggggcag cagttgccgt gtttttgatc atagcaggag
     8941 gagttgcggg tctatatttt catggaaaaa attcgtcaga agcgaaaagt acgaccataa
     9001 cggaaacaac gacaaagaaa acagagacga aacaaacaag tgagtcatca actaaaacaa
     9061 aagagacaca aacttctgaa gaaaaaacag caaatggttt gccagagcaa cttcaagcaa
     9121 tcgtaaatga ttattcgaaa aagatcgaag aaaagacgcc tgcattgatt gaagaatacc
     9181 agacagaaat tcagggaaat caagagggaa tagctggttt atcagcagtt gctaaccaaa
     9241 aagcacgtga gcttcaagca atctccgacg aagggatcag aaaattacgt gcagcgtatc
     9301 aggcagcaga aaataaagac ggtgtagact tagatacttt gatcaatcaa ttatcggcta
     9361 attatacgaa tcatgtcgct aagatttccg atatttattt gcagactagc gcctctcttc
     9421 aagcagagtc aacaagcaca caagacacaa catcttctga ttctgagaca gcagagtcgg
     9481 tagaagcttc tgaaatgcaa gctcgaacaa cacaagacac aacagattcc tctgaaacta
     9541 ataccaacag cgcgacttca caggctgcga caaccgtagt tttggaagga gaaggtccaa
     9601 atcaaatagc cgaacgaact ggcgtaccag tagaaacgat cttgtcgctg aatgggatga
     9661 gcatggagaa ttatttcctg actcccggtg agacgctaaa attgaattga taaaagcaat
     9721 aaaaaagaac gaagcttcca ctaaggtgaa gcttcgttct ttttttattt tccggttctt
     9781 ttttcgcgta caaagtaatg gatcgtcatg taaagcaaga aaacgagtcc aataatcaat
     9841 gagattctcg tttcaggatt gataaacatg aaaatcaata cgatagctaa aaaagcaagt
     9901 gtcacataat tcgtataagg agatagtggc attttaaaag gatgatcatg cattttatct
     9961 gcgtgcttct ttttgaagcg aatctcgctg atcaaaatca ctacccaagg aaccatacca
    10021 ggcaaaatac ttgagctgta tacgaaaacg aaaatatttt ctgactggtt gatgaacatt
    10081 ggtagaagat aatttaagat cagaccaatt aagattccac ctgagattgc tacgacagga
    10141 taaaaaggcg caccgtgacg agatagtttc agaaattttt tagacatctt tccatttatg
    10201 gcaagtgtat agaccatacg acttgcgctg aaaatccctg aattgcaacc tgataatgcc
    10261 gctgtaatca cgacaaaatt aatcagtcct gcagcaaatg taatgccgac tttagaaaaa
    10321 gtttgtacaa aaggtgaacc aatctcactc aattgattcc acggatagat actcacaatg
    10381 ataaaaatag caccaatata aaaaatcaag attcgaccga tagtggactg gacagcttca
    10441 atgatcgtct ttttaggatt ctctgcttca ccagcagtca ttccgatcag ttcgattcct
    10501 tgataggaag caacaacgat cgataatgca aagaaaaatc ctttgacgcc tccagtgaag
    10561 aatccaccat ttttccaaag atttgaaatc cctactgcct gaccatgatt accaaatcca
    10621 aataagatta ccccaagacc tgcaatgatc atcaaaatga tcgttatgac tttgatgatc
    10681 gaaaaccaaa attctaattc gccaaacgcc ttgacagaaa taagatttgc cagcataaga
    10741 aacaagacga caatgattcc gggaataatt gccggcaaat caggccacca ataattcata
    10801 taagatccta cagcaatcac ttcacttatt cctacgacaa tatattgaaa aatattactc
    10861 caagcggtca agtagccagc cacaggatgg atgtactcgc tcgcgtaatt tgcaaaagaa
    10921 ccagtcgaag gatcaacgta aagcatttcg cctaaagcac gcataatcaa atacaaaaag
    10981 aatcctgcaa tcgcataggc tcccatcact gagggccctg tccaacgtat ggttgaggag
    11041 gcacccataa acagaccgac accgatcgtt ccacctagtg cgatcatttg gatatgacgg
    11101 gcactaagac ttcgagttaa cttttcttct tccatacaaa aactcctttc gttgttgagt
    11161 caacaagaaa aaataatcaa acgtttcaac acatgttttt cattattagg ttagccttat
    11221 tgaactcttg atgtgattct acagaaaatt ttcataaaat gcgaggaaaa ttcgaaaaaa
    11281 actttttcat cattctactt gaaaatgctt tccattttag ttaaactata tttaaataaa
    11341 aagttaggta aacctaaaaa ggagtgtaag atttggaacg tacggccatt gtaattcaag
    11401 acctttcagc cgcttatcaa gggaaaactg ttttgaaaac tatcaacttg acgataaatc
    11461 cacaaaaaat taccggtatt atcggaccaa acggtgcagg aaaatcaaca tttatgaaat
    11521 ctttgctaga gttgatacca gctactactg gtaaagtcac cttttctgga cagccggtaa
    11581 actcagtccg taaaaaaata gcctacgtgg aacagcgaag tgaattggat ctttcctttc
    11641 caattgatgt attaggcgtt gtacttttag gaacatatcc atctttacga attggacaaa
    11701 gacctgggaa aaaagaaaaa gaacgtgcaa gacaagcttt gaaaaaagta gggatggaag
    11761 aatatgccaa aagacagatc agcgaactat cgggtggaca gcttcagaga gtttttattg
    11821 caagagctct agcccaagga gcagaatgga tctttttaga tgaaccattc gtagggattg
    11881 atgcgttaag tgaacgaaag atctttgaca tcttgcagga attgaagaat tcaggaaaaa
    11941 cgattttgat cgtccatcat gatcttcata aagtagaaga atatttcgat gaggttattc
    12001 ttttaaataa acagctgatc gcttccggtc cagtacaaga agtctttaca tcgaagaacc
    12061 ttcaattggc ctatggtgag atcatcagac atttagtaaa aggaggagag aaaaaatgat
    12121 ccaatccttt attgatggat taatgaatta tcaattcctg caatacgctt tggtcacttc
    12181 catgcttgta ggacttgctt ctggagtcat cggttctttt attattttac gagggatgtc
    12241 tttgatggga gacgctatct ctcacgcagt tcttccaggt gtagccattt cttatatgtt
    12301 tggttttagt tacatctttg gagccactgc ttttgggatg ctaacagcag cagtgattgg
    12361 tttcgtgact cagcggagcc gattgaaaaa tgatacagcg attggaattg tctttagctc
    12421 cttttttgca ttagggatta tcttgatttc atatgcccag agtgcgacag atttgtatca
    12481 tattttattt gggaatgtct tagctgttag ggaaagcgat ttgttgctga cagcactcgt
    12541 tagtggaatc gtcttgatat ttgtcttttt cttttataaa gaattgaaga tcacatcatt
    12601 cgatccgaca atggcaaaag cttatgggct gaacacatcg ctgatccatt atcttttgat
    12661 gttctttttg acattagtgg ctgtagtcag tttacagaca gtaggaacaa tcttggtgat
    12721 tgccatgttg atcacaccag ccgccacggc ttacttgcta acgaaccatt tactgaaaat
    12781 gatcattaca gctgcaggaa tcggtatgct aagtgcagtt gtcggtgtgt ttttcagtta
    12841 tagttacaat tggccatcag gagctacgat cgtgttagca tgtaccgcat tttttatcct
    12901 tgctttttta ttttctccaa caaaaggaat tttatttcag aaaggaagca gatagatgaa
    12961 aacaaaaaaa tcactctttt taatcttggc agttagtttc ttggtattag ccggttgcgg
    13021 aaagcaggct tctgaccaag cggatgaagg aagtaaagaa aagttgtcag tcgtggctac
    13081 caattcgatc ttggcggaca tggcaaaaga ggtcggaaca gatcaaatag atatccacag
    13141 tatcgtacca gtcggaacag atccgcatga atatgaagta ctaccagaag acatcaaaaa
    13201 ggcaagcgat gcagatgtta ttttatacaa cggtttgaat cttgaaacag gtaacagctg
    13261 gttcgataac ttgatggaaa cggctaaaaa agaagaagga aaagattatt ttgcagttag
    13321 caaaaatgta gaacctcttt atttaactag cggtgaagaa catacaaaag cagatcccca
    13381 tgcttggcta gacctatcta acggaataaa atatgtggag gaaatcgcac gtatattctc
    13441 tgaaaaagat gcagaaaatg cgacactcta taaaaaaaat gcagaagcat atgtggaaaa
    13501 actaaaagaa ttagatacac aagcgaagga aagttttgct tctatcgaag agaacaaaaa
    13561 attattagta acaagtgaag gggcattcaa gtatttttca cgagcatatg atttgccagc
    13621 agcttatata tgggagatca atacagaaag tcaaggtacg cctgatcaaa tgaaagcgat
    13681 cattgatcag ataagagcaa aagaagtacc ggttttattc gtggaaacca gtgtagactc
    13741 aagaagcatg gaacgggtag caaaagaaac gggtttgaaa atctatgata aactgttcac
    13801 tgattccata gcaaaagaag gagaacaagg agattcttac tatcagatga tgaaatggaa
    13861 tattgaaacg atccatgaag gtttgtcaca aacaaaagaa agctgaaaag tgcaaggtct
    13921 agcttgagga gcagaccttg cattttttat aagagatatg aaaaaaaatt atgctgtttt
    13981 tcgtcgtaca gatacctttt ttgaagaaat tccgttatag ttagagtaag gaaggaaggt
    14041 gtggctatga attttacacg taaagataaa tatacgatta aagaagaagt ctttcgattg
    14101 atcagccagc aggaaaatac cgacaaggat caagaccttc aatttcttga agcagaatta
    14161 gctaaattat tcatagcaaa tcaaaatgag acaacagaac cagatgaaga gctgtacata
    14221 ggcaaatggt tgtttgatga atttcagcct ttagatgaaa ataccggtat cggttatttt
    14281 gtgttccttt ctccagtcgg cttcacacct ggcgtattag ttaaacaaaa cgacgaaata
    14341 accgtcaaag catggcctgc attattttca gtcatcaaag cagaaggagt aatcaaaaag
    14401 atccagtcgg cctattataa aagtcaattt attgccaaca gcaaattaat gaatatgttt
    14461 tataatcgtc tagtagaaat tgctaggcct gggaaaatcc cgcaaacatt cacgtacaca
    14521 cagcaaaaaa atctctatga agaagtattg tattcccgcg atctgtctga gttttcgcca
    14581 tgggaagagg taaggctcca tgaactgtta agtacaaaac cagtttcatt gaattttttt
    14641 aatgcattag ataacaaaag aaaattaatt tcaacaaatt attgggaagt atttgctgca
    14701 agaaaaaaat ggatggaaga aaagaagatt gccgatcagc agatccagtt tgtccagctg
    14761 aaaaaagaaa aactatatta tgggttatac agccgttaac agcaagcgag gaagaccatg
    14821 acattaaaag aagaaatcat acaagaaagc aaacgattag gaattgacaa aatcgggttc
    14881 accacggcgg aaccttttga ttcattaaaa gaatcgttgg aagaacaaaa agcagctggc
    14941 catacttctg gctttgaaca tccaaatatc gatgagcgtc tttatccaga aatgacattt
    15001 gatcagccga aatcgatcat ttccattgca cttgcgtatc ctactaggat ccatgaagaa
    15061 gtgcctaggg atgaaaaacg cgggcagttt gctcgtgctt cttgggggat cgattaccat
    15121 gatatactca gagaccgttt gaaccgattg atcgaattca tccaaacaag agcagaaaaa
    15181 tggcaaaaag aagaggaatg gcgtctagct ccgcaagtag atactggaga actgatcgat
    15241 gtcgctgtag cacagcgagc tggtttagga tttattggac gaaacggatt gttgatcact
    15301 gaagagttcg gttcatttgt ctatcttggt gagatcgtta ctaatattgt atttgagcct
    15361 gataaaccag gagaatttgg ttgcggtgac tgtatgcgct gcgtgactgc ttgtccgaca
    15421 aaagcattgc ttggtgacgg aagaatgaat gcccagcgat gtttgtctta tcaaactcag
    15481 acaaaaggga tgatgccgga agaatatcgt aaaaaaatgc gaaatgtcat ttatggatgt
    15541 gatatctgcc agttagtctg cccttacaat caaggaaaag attttcattt tcatgaagag
    15601 atggaaccaa aagtagacga agtatatcca aaattaaagc caatgctttc gatgtctaat
    15661 aaagacttca aacagcaatt tgggcattta gctggttcat ggcgtgggaa gaaacctttg
    15721 cagagaaacg cattgattgc tttagctaat ctaggtgatc gaacggcatt gcctgagatt
    15781 tcagaatgtt taacagatgt ccgccctgtc atcagaggaa cagcagcttg ggctattgga
    15841 cgtctaggaa caaaagaacc ggaaaaatgg cttgatgtat tagatgaaat attagctaaa
    15901 gaaacagaag aagtcgttat aaacgaattg gaacatgccc agaaattatt gcggagaaag
    15961 ttaaaataaa aaaatagtta acattattca aagtttttgt ccagtaattg ttgtaacaat
    16021 tactggtttt tttgtatttt ttacattttt ttaagagaat ttgataaaaa tcaaaaattt
    16081 ttttgctttt tttta
//
LOCUS       DARBET010000058        15953 bp    DNA     linear   BCT 07-NOV-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4027
            SAMEA114569841-rid20112183.denovo.060, whole genome shotgun
            sequence.
ACCESSION   DARBET010000058 DARBET010000000
VERSION     DARBET010000058.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA114569841
            Sequence Read Archive: ERR12203666
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 15953)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15953)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001972618.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001972618.1
            Genome Coverage       :: 132x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/07/2023 13:18:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,041
            CDSs (total)                      :: 2,975
            Genes (coding)                    :: 2,908
            CDSs (with protein)               :: 2,908
            Genes (RNA)                       :: 66
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 67
            CDSs (without protein)            :: 67
            Pseudo Genes (ambiguous residues) :: 0 of 67
            Pseudo Genes (frameshifted)       :: 26 of 67
            Pseudo Genes (incomplete)         :: 28 of 67
            Pseudo Genes (internal stop)      :: 20 of 67
            Pseudo Genes (multiple problems)  :: 6 of 67
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15953
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA114569841-rid20112183.denovo.060"
                     /isolate="VRE4027"
                     /db_xref="taxon:1352"
                     /country="Denmark"
     gene            complement(390..812)
                     /locus_tag="SCJ86_002054"
     CDS             complement(390..812)
                     /locus_tag="SCJ86_002054"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit epsilon"
                     /protein_id="HEG1169587.1"
                     /translation="MDQYLTVNVVTPAGLVYDHHAAIVVARTTAGELGILPKHAPIIV
                     PLTIDEVRVKRTDSDTHVDWIAVNGGIMEVRDNVVSIVADSAERERDIDVSRAERAKQ
                     RAERQIEEAKAKQDTNELKRATVALHRAINRIKVSKHS"
     gene            complement(826..2232)
                     /gene="atpD"
                     /locus_tag="SCJ86_002055"
     CDS             complement(826..2232)
                     /gene="atpD"
                     /locus_tag="SCJ86_002055"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016251817.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit beta"
                     /protein_id="HEG1169588.1"
                     /translation="MSSGKIVQVIGPVVDVEFSLDQSLPDINNALVVYKNDENKSKVV
                     LEAALELGDGVIRTIAMESTDGLQRGMEVIDTGKAISVPVGKETLGRVFNVLGDTIDL
                     ETPFPEDAERSEIHKKAPAFDELSTSTEILETGIKVIDLLAPYLKGGKVGLFGGAGVG
                     KTVLIQELIHNIAQEHGGISVFTGVGERTREGNDLYYEMKDSGVIEKTAMVFGQMNEP
                     PGARMRVALTGLTIAEYFRDVEGQDVLLFIDNIFRFTQAGSEVSALLGRMPSAVGYQP
                     TLATEMGQLQERITSTKKGSITSIQAIYVPADDYTDPAPATAFAHLDATTNLERKLTE
                     QGIYPAVDPLASSSSALAPEIVGEEHYKVATEVQHVLQRYRELQDIIAILGMDELSDQ
                     EKVLVSRARRVQFFLSQNFNVAEQFTGLPGSYVPVEETVKGFREILEGKYDDLPEEAF
                     RSVGRIEDVVEKAKTLGY"
     gene            complement(2255..3157)
                     /locus_tag="SCJ86_002056"
     CDS             complement(2255..3157)
                     /locus_tag="SCJ86_002056"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623451.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit gamma"
                     /protein_id="HEG1169589.1"
                     /translation="MGASLNEIKTRIASTKKTSQITRAMQMVSASKLTKSEASSQKFQ
                     VYATKVREIVTHLTATQLNDIASENPRGDINYNSMLLSRPVKKTGYIVITADGGLVGG
                     YNSSILKQTMSILEEDHQSPDDYVMIAIGGTGADFFKARGINLAYELRNLSDQPSFDE
                     VRKIVNMATTMYQNEVFDELYVCYNHHINSLTSQFRVEKMLPISDLDPEEATTFEQEY
                     IFEPSKEEILSQLLPQYAESLIYGAIVDAKTAEHAAGMTAMKTATDNAANIIDELTVS
                     YNRARQGAITQEITEIVAGASALE"
     gene            complement(3171..4727)
                     /locus_tag="SCJ86_002057"
     CDS             complement(3171..4727)
                     /locus_tag="SCJ86_002057"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005878464.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit alpha"
                     /protein_id="HEG1169590.1"
                     /translation="MAIKAEEISALIKEQIENYQNVLSVEEIGTVTYVGDGIARAHGL
                     ENAMSGELLEFSNGSYGMAQNLESNDVGIIILGDFESIREGDKVKRTGKIMEVPVGEA
                     LIGRVVNPLGQPIDGLGEIVTDKARPVEAMAPGVMQRKSVNEPMQTGLKAIDALVPIG
                     RGQRELVIGDRKTGKTSIAIDTIINQKGQDMICIYVAIGQKDSTVRTQVETLKKYGAM
                     DYTIVVNAGASQPAPLLYIAPYAGTAMGEEFMYNGKHVLIIFDDLSKQAVAYRELSLL
                     LRRPPGREAYPGDVFYLHSRLLERAAKLSDDLGGGSMTALPFVETQAGDISAYIPTNV
                     ISITDGQIFLESDLFYAGTRPAVDAGLSVSRVGGSAQIKAMKKVAGTLRLDLASYREL
                     EAFTQFGSDLDAATQAKLNRGRRTVEILKQKLHAPLAVEKQVVILYALTHGFLDSIPV
                     DSILDFEHELFEYLDTNHSDIFETIRTTKDLPEEEALNSAIQEYKDMFLATKGNNSST
                     EDKLKSIQNA"
     gene            complement(4750..5292)
                     /locus_tag="SCJ86_002058"
     CDS             complement(4750..5292)
                     /locus_tag="SCJ86_002058"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010752517.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit delta"
                     /protein_id="HEG1169591.1"
                     /translation="MKLDKYTVGRRYGKALFELAIDSNKAEEIYQDLVSLRQIYEQVP
                     GLGNMLSDVRLEPHEKREIMDKLVSGYDGIVKNFLEVVYAYNRMDDLLFMIEEYERRY
                     DEHQGLLLGSVTTAVPLSDEQRIQLEKNVAKTMNYQSVELKQIVDSSIIGGAIVEANH
                     RVIDGSIRTQLENMRNQLNR"
     gene            complement(5279..5803)
                     /gene="atpF"
                     /locus_tag="SCJ86_002059"
     CDS             complement(5279..5803)
                     /gene="atpF"
                     /locus_tag="SCJ86_002059"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734887.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit B"
                     /protein_id="HEG1169592.1"
                     /translation="MLNQLAIAEVGNAMLGNVIVVSGSFLILLALLKHFAWGPISDIL
                     KKREDKIANDLDSAEQSRINSAKMEQEREQQLLASRSDAADIIKNAKESGELSRQNIL
                     KDAQEEAARLKSKAQADITVERDSALNSVKDDVAELSLQIAEKILNKELSPEMHESLI
                     NQYIEGLGSSNETR"
     gene            complement(5894..6109)
                     /gene="atpE"
                     /locus_tag="SCJ86_002060"
     CDS             complement(5894..6109)
                     /gene="atpE"
                     /locus_tag="SCJ86_002060"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010752519.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP synthase F0 subunit C"
                     /protein_id="HEG1169593.1"
                     /translation="MNYIAAAIAIMGAAIGAGYGNGQVISKTIESMARQPEMSGQFRT
                     TMFIGVALVEAVPILGVVVALILVFGV"
     gene            complement(6162..6881)
                     /gene="atpB"
                     /locus_tag="SCJ86_002061"
     CDS             complement(6162..6881)
                     /gene="atpB"
                     /locus_tag="SCJ86_002061"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734885.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit A"
                     /protein_id="HEG1169594.1"
                     /translation="MDERSLTFHIGPIWFDGTVCIMVLLTCLIVFFLAYFFTRNLKLK
                     PTGKQNAIEWVIDFSRGIVTDNLPRKELSNFHLLAFTLFLFVFVANNIGLVTKIVLPN
                     ETTLWKSPTADPFVTLTLAFIMILLTHLFGVKKLGFKGYFVNSFLKPYSFMFPMKLIE
                     EFTNLLTLALRLYGNIYAGEVLLTLIAKMVISLGWFSLPLAIPLEMVWIAFSLFIGSI
                     QAFVFVTLSMVYMSHKIEVEE"
     gene            complement(7183..10026)
                     /gene="rnr"
                     /locus_tag="SCJ86_002062"
                     /pseudo
     CDS             complement(7183..10026)
                     /gene="rnr"
                     /locus_tag="SCJ86_002062"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002418328.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease R"
     gene            complement(10029..10784)
                     /locus_tag="SCJ86_002063"
     CDS             complement(10029..10784)
                     /locus_tag="SCJ86_002063"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HEG1169595.1"
                     /translation="MRRVRQLPKPLYAKHGKRAVLLLHAYSGSPNDVRMLARFLEKAD
                     YTVYAPLFKGHGTMEPYDILQEKAESWWADTKKAIHFLQSEQFSDIAVLGLSMGGIFA
                     VRALEEESVIGGGFFCSPLSPVKTNVPENFEKYVRQVLKTAGKSEKEINEKAVAYRSL
                     AEQQLMDIQDQAAIVESRLSDIQQPIFLAQAGKDEMIDPNGVFETARKLSRQRVTLRW
                     YPESGHVITVGTARRELEKDVLEFLEKLPWNEE"
     gene            complement(10887..11123)
                     /gene="secG"
                     /locus_tag="SCJ86_002064"
     CDS             complement(10887..11123)
                     /gene="secG"
                     /locus_tag="SCJ86_002064"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010718604.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecG"
                     /protein_id="HEG1169596.1"
                     /translation="MYNLILGIVIVLSIVMVIAIMMQPSKQNSAASAFTGGADQLFGK
                     QKARGFEAVMQRSTAVMGAIWMILLFVLAYLSSK"
     gene            complement(11241..11519)
                     /locus_tag="SCJ86_002065"
     CDS             complement(11241..11519)
                     /locus_tag="SCJ86_002065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377463.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetyltransferase"
                     /protein_id="HEG1169597.1"
                     /translation="MEIKEEKNRLALFNDEQQEIGEMTWSDAGPDIMIIDHTFVDPAY
                     RGQKLAEKLVYTGVELARRDGKKIIPLCPYAKKEFEKKPEYQDVLRQS"
     gene            complement(11632..12600)
                     /locus_tag="SCJ86_002066"
     CDS             complement(11632..12600)
                     /locus_tag="SCJ86_002066"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008268520.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HEG1169598.1"
                     /translation="MVIFYFILILIIIGISYHFLSYFYAKKLVRAGVQRGYDWYEDTG
                     HRLMDPAAVTSVEKKRQKLEEECESFWEQGTPKTIKSYDGLKLAGQMFIHPNKQNKWV
                     ICVHDYRSTGKRDMSHIGKRYAEKGFNVLVPDLRAHGESEGEIIGMGWLDRLDLIAWI
                     QLILDEQPNASIILHGGSMGASTIMMASGEKLPSAVKGFILDSGYVSVYAEFRYMLSK
                     ITVFPKKMVMRYANHYAQKYAGYSLKQASATRQLGSNHLPLLVIHGERDHFVPTEAAY
                     TIQNATAGDKALLLVPEAEHLEASMKDPKTYWTVIFSFIEQRITMN"
     regulatory      12815..12859
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00522"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="PreQ1 riboswitch class I; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="preQ1"
                     /db_xref="RFAM:RF00522"
     gene            12883..13857
                     /locus_tag="SCJ86_002067"
     CDS             12883..13857
                     /locus_tag="SCJ86_002067"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside hydrolase"
                     /protein_id="HEG1169599.1"
                     /translation="MRKVIIDCDPGIDDTLALSLAVKSPDIEVVAITVVCGNVPADIG
                     TQNVLKCLERCDRLDIPVYQGMEKPLKQPFISAQDTHGMDGLGETNFPMILRKQAEPL
                     HAVDFLADYFKEKTNTSVIALGPLTNIAFALKVNPNIGKHMDRFVSMGGTYKSHGNCS
                     PVAEYNYWCDPEAASYVFENLKQTIEMVGLDVTREIVLTPTILEYCCQMNPEEGEYLK
                     AITRFYFDFHWKQERILGCVINDPLAVAYFIEGAICEGFKSFTAVETQGISRGQTLVD
                     RYEFWQKPANSKIMTKVDTRLFFRKFLTVLLNAQEETIMKDLERLKMG"
     gene            13862..14437
                     /locus_tag="SCJ86_002068"
     CDS             13862..14437
                     /locus_tag="SCJ86_002068"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ECF transporter S component"
                     /protein_id="HEG1169600.1"
                     /translation="MNKNKMATRVLTVMALCIGINYIGGTIALWLRLPIYLDSIGTIF
                     AGALLGPVPGMLTGLSSGILSGVTTDIFSLYYSPIQILTGLLAGLILPRYLGKDKNSL
                     KLLLLALILSVPGTIVSSIITVQLFGGITSSGSSMIVQLLHGLGLSQTTSVILVQAGT
                     DYLDRLLSIVVVSLVITMLPQRLIYSKRKIG"
     gene            complement(14504..15643)
                     /locus_tag="SCJ86_002069"
     CDS             complement(14504..15643)
                     /locus_tag="SCJ86_002069"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314669.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ArgE/DapE family deacylase"
                     /protein_id="HEG1169601.1"
                     /translation="MKKEEKIAILQEIIRIKSVNGNEGEVAAYLNKLLAKHDITGEIV
                     SYRDGRDNLIARYQKGQSGKVLGLSGHMDVVAAGDESSWTYAPFAAEIHGNRLYGRGA
                     TDMKSGLAAMVIAMIELKESGKPFNGTVKLLATVGEEVGELGGEQLTKAGYVDDLDAL
                     IIGEPTNYSLMYTHMGSINYTVTSHGKEAHSSMPDQGYNAINHLNEFITKANAEMNHL
                     AETIENPVLGKTIHNVTLISGGNQVNSIPSHAQLQGNIRSIPEYPNDKIIALLQSIVN
                     ELNQETDYHLELMIDYNKIPVKADPDSPLIHCIQQQFSQPLPLVGAAATTDAAEFTKA
                     NHSFDFVVFGPGVVTLPHQVDEYVEIDNYLDMIEKYQGIILSYLA"
BASE COUNT         4359 a         3443 c         2856 g         5295 t
ORIGIN      
        1 aatctgagcc agaatgtggt tcattctgac tcagatttga ttttgcacaa ctcatcagtc
       61 cttattgaca aagagcccaa aagtcttgtc gcagtccctt attccaaata aacggtgttc
      121 aatcatctga ccctcggtat tagctcaaaa aacggcaaaa tatgaggaac tattttccat
      181 ttgtttctct aatactcagg acaaaacgct gatttcacaa cctccgatat acggtgttcc
      241 agaagtagct tcttcgaaaa ataagctgag atttcacaaa aatttgagaa gcaattttcg
      301 taaattcctt cttatgtctt gaagctgacc acttctgtca taacctcttt ttactgttct
      361 taaacacgga taaacatccg catctcattt tatgagtgct tagatacttt gatacgattg
      421 attgctcggt gcaacgcaac agttgcccgt ttcaattcgt tcgtatcttg ttttgctttt
      481 gcttcttcga tttggcgttc tgcgcgttgt tttgcacgtt ccgcacgaga tacatcgata
      541 tcacgttcac gttccgcact atcagcaacg attgaaacaa cattatcacg aacttccatg
      601 attccgccat taacagcgat ccaatcgacg tgggtatctg aatctgttcg ttttacacga
      661 acttcatcaa tcgtcaatgg aacgatgatt ggtgcatgtt taggcaaaat cccaagctca
      721 ccagctgtcg tacgtgcaac gacgatagct gcatggtgat catagaccaa accggcagga
      781 gttaccacat ttactgttaa atattgatcc atggggcacc cctttctagt agcctagtgt
      841 tttcgctttt tctacgacgt cttcgattcg gccaacactt cggaaagctt cttcaggaag
      901 atcatcgtat ttgccttcca agatttcgcg gaatcctttc actgtttctt ctacagggac
      961 atatgaacct ggaagaccag taaattgttc agccacgtta aagttttgtg acaagaagaa
     1021 ttggactctg cgggcacgag aaactaacac tttttcttga tcggataatt catccatacc
     1081 taagatagca atgatatctt gtaattcacg atatcgttgc aatacgtgtt ggacttctgt
     1141 tgctacttta taatgttctt ctcccacgat ttcaggtgct aaagcacttg aagaagaagc
     1201 caatggatct accgctggat aaatcccttg ttctgttaat ttacgttcca agttggttgt
     1261 cgcatccaaa tgggcgaatg ctgttgccgg cgccgggtca gtgtagtcat cggctggcac
     1321 atagattgct tggatcgatg tgatcgaacc ttttttcgta gatgtgatac gttcttgcaa
     1381 ttgacccatt tctgttgcca atgttggttg ataaccaacc gcagaaggca tccgtcctaa
     1441 caaggctgat acttcagatc cagcttgagt gaaacggaaa atgttgtcga taaacaacag
     1501 tacatcttga ccttctacat cacggaaata ttccgcaatc gtcaaaccag tcaaagcaac
     1561 acgcatacgt gcacctggtg gttcgttcat ttgtccgaac accatggctg ttttttcgat
     1621 aactcctgaa tctttcattt cataatacaa gtcattacct tcacgagtac gttcacctac
     1681 accggtaaat acagaaatac caccatgttc ttgggcgata ttatggatca gttcttggat
     1741 cagtacggtt ttaccaacac cggcaccacc gaatagtccg actttcccac cttttaaata
     1801 tggggcaagc aaatcgataa ctttgatccc tgtttccaaa atttctgtac ttgtacttaa
     1861 ttcgtcaaag gctggtgctt ttttatgaat ttcgcttctt tccgcatctt ctgggaaagg
     1921 tgtttctaaa tcgatcgtat ctcctagtac gttgaatacg cgacctaatg tttctttacc
     1981 tacaggaaca gagattgctt tgcctgtatc aatgacttcc attcctcttt gtaaaccatc
     2041 tgttgattcc atcgcaatgg tccggatcac tccgtcccct aattctaacg ctgcttcaag
     2101 aacaactttt gatttatttt cgtcattttt ataaacaact aatgcgttgt tgatatctgg
     2161 taaggattgg tctaaagaaa attccacgtc gacaacggga ccgattactt gaacaatctt
     2221 gcctgaactc atcttttttc ctccaatctc aatactattc tagtgccgaa gctccggcaa
     2281 caatttcggt aatttcttgg gtaatagccc cttgtcttgc acggttgtaa gaaaccgtca
     2341 gctcatcaat gatattagca gcattatcag ttgctgtttt cattgctgtc ataccagcag
     2401 catgttctgc tgttttggcg tcaacgatcg caccgtagat caaactttca gcatactgag
     2461 gcaataactg agacaagatt tcttcttttg aaggttcaaa aatatattct tgttcgaaag
     2521 tagttgcttc ttctggatct aagtctgaga ttggcagcat tttttctaca cgaaactgac
     2581 tagttagcga attgatatga tggttataac atacgtataa ttcatcgaat acttcgtttt
     2641 ggtacatagt tgttgccata tttacgattt tgcggacttc atcaaagctt ggctgatcag
     2701 atagattgcg cagctcatat gctagattga taccgcgtgc tttaaagaag tctgcaccag
     2761 ttccgccaat tgcgatcatc acgtaatcat cgggtgattg gtggtcttct tctaaaatag
     2821 acatcgtttg tttcaatatc gagctgttat atccaccgac taaaccgcca tctgcagtga
     2881 tgacgatata acccgttttc ttaacaggac ggctgagcaa catgctgttg taattgatat
     2941 cccctctagg attttctgag gcaatatcat ttaactgagt agcagtcaaa tgcgtaacga
     3001 tttctcttac ttttgttgca taaacctgaa acttctgtga agaagcttca gattttgtca
     3061 acttagaagc agacaccatc tgcattgccc gcgtgatttg gctcgttttt ttcgttgaag
     3121 cgatacgtgt cttgatttca tttaatgaag cacccattca tctcacctca ctatgcattt
     3181 tgaatggatt tcaatttatc ttctgtagag gaattatttc cttttgtagc taagaacatg
     3241 tctttatatt cctgaatcgc actgttcaac gcttcttctt ctggaagatc ttttgttgta
     3301 cggattgttt caaagatatc tgaatgattg gtatccagat attcgaataa ttcatgttcg
     3361 aagtctaaaa tactgtctac agggatacta tctaagaatc catgtgtcaa agcatacaaa
     3421 atcactactt gtttttcaac agctagcggt gcatgcaatt tttgtttcaa gatttctact
     3481 gtacggcgtc cacgatttag ttttgcttgt gttgctgcat ccaagtcaga accgaattgt
     3541 gtaaatgctt ctaattcacg gtaacttgct aagtctagac gtaaagtccc agcaactttt
     3601 ttcattgctt tgatttgcgc agaaccccct acacgagata cagatagtcc ggcatcaacc
     3661 gctggacgag tacctgcgta gaacaagtcg ctttctaaga agatttgtcc atcagtgatc
     3721 gaaataacgt ttgttgggat ataagcagag atatctccag cttgtgtttc aacaaatggt
     3781 aaggcagtca ttgaaccgcc acctaaatca tcacttaatt ttgctgcacg ttccaataag
     3841 cgtgagtgta agtagaaaac atcccctgga taagcttcac gacctggtgg acggcgaagc
     3901 agcaaggaaa gttcacgata agcaacagct tgttttgaaa gatcatcaaa aatgatcaag
     3961 acatgtttcc cgttgtacat gaactcttca cccattgcag taccagcata tggtgcgata
     4021 taaagcaacg gcgctggttg agacgcaccg gcattcacaa cgattgtgta atccattgcg
     4081 ccatattttt tcaatgtttc aacttgtgta cgaactgtag aatctttttg tccgattgct
     4141 acatagatac agatcatatc ttgacctttt tggttgatga tcgtatcgat cgcaatggaa
     4201 gttttccctg ttttacggtc accgataact aattcacgtt ggccgcgtcc gattggtaca
     4261 agagcgtcga ttgcttttag acctgtttgc attggttcgt taacagattt acgttgcata
     4321 acgcctggtg ccattgcttc tactggacga gctttgtcag taacgatttc acctagtccg
     4381 tcaattggct gacctagcgg attaactacc cgtccaatca aggcctctcc aactggaact
     4441 tccatgatct ttcctgttcg ttttacttta tctccttcac gaatggactc gaaatcgcca
     4501 aggataatga tacctacatc atttgattct aagttttgcg ccattccgta tgagccattt
     4561 gaaaattcaa gcaactcacc actcatcgcg ttttctaaac catgtgcacg agcgatcccg
     4621 tcacctacat aagttacggt accgatttct tcgactgaaa gcacgttttg ataattttca
     4681 atttgttctt taatcaaggc actaatttct tctgctttga tggccattcg attcacctac
     4741 ctctttgtac tatctattta attgattacg catattttct aattgcgtgc gaatgcttcc
     4801 gtcgattact cgatggttag cttcaacaat cgcgccacca ataatagaag aatcaacgat
     4861 ttgttttaat tcgactgatt gatagttcat tgtttttgca acattctttt ctaattgtat
     4921 tcgttgttcg tctgaaagtg gaactgctgt tgtgacactt cccaacagca aaccttgatg
     4981 ttcatcatat cgacgttcat attcttcgat catgaacaaa agatcatcca ttcggttata
     5041 cgcataaaca acttctaaaa agtttttaac gattccatca tagccactaa caagcttgtc
     5101 catgatttct cgtttttcat gaggttctag acgaacatca cttaacatat tacctaatcc
     5161 aggtacttgt tcatagattt gacgtaagct tacgagatct tgataaattt cttctgcttt
     5221 atttgaatcg atcgccagtt caaataaagc tttcccgtaa cgtcttccta ctgtatattt
     5281 atctagtttc atttgaagaa cctagacctt cgatatattg attgattaat gattcgtgca
     5341 tttctggaga taattctttg ttcaagattt tttccgcgat ttgaagagat agttctgcaa
     5401 catcgtcttt tacagagttc agcgctgaat cacgttctac agtgatatca gcttgggctt
     5461 tactttttag acgagctgct tcttcttgag catccttcaa aatattttgg cggcttaatt
     5521 ctccactttc tttcgcattt ttgatgatat cagctgcatc agaacgagag gctaacaatt
     5581 gttgttcgcg ttcttgttcc attttcgctg agttgatgcg agattgttct gcagaatcca
     5641 aatcattggc gatcttgtct tcacgttttt tcaaaatatc gctgattggt ccccaagcaa
     5701 aatgcttcaa gagagccagt agtatcaaaa atgagccgct gacaacgatg acatttccta
     5761 acatcgcatt gccaacttct gcaattgcca attgattcag cataggcgag ccttcctttc
     5821 tttgaagtac ttctagtttt catgtaaaaa agcagtaaca aaccaaattg tttctgcctt
     5881 aatactgatt caattaaaca ccaaaaacta gaatcaaggc gacaactacc cctaagatag
     5941 gaacagcttc gactagagct accccgataa acattgttgt acggaattga ccagacattt
     6001 caggttggcg agccattgat tcgattgttt tagagataac ttgaccgtta ccataaccgg
     6061 ctccgattgc tgctcccatg attgcgattg ctgctgcgat ataattcata attgttttcc
     6121 tcctaaatta gataaatagt agtttgtttt taaaacgaat attattcttc cacttcaatc
     6181 ttatggctca tataaaccat agataaagta acgaagacaa atgcttggat gcttccgata
     6241 aacaaggaga atgcgatcca gaccatctct aatggaattg ctaacggcaa tgagaaccag
     6301 cctaagctta ttaccatttt tgcaatcaag gttagcaaaa cttcccccgc ataaatattc
     6361 ccataaagac gtaaagcaag tgttaataag tttgtaaatt cttcaatcag tttcatcgga
     6421 aacataaaac tatatggttt caagaacgaa tttacgaaat atcctttgaa gcctaacttt
     6481 ttcaccccaa ataagtgagt aagtaagatc attataaatg ccaaagttaa agtaacaaac
     6541 gggtctgctg tcgggctttt ccaaagtgtt gtttcgttag gtaaaacgat ttttgttact
     6601 aaaccgatgt tgtttgcaac aaaaacaaat aaaaacaatg taaatgccaa caaatggaaa
     6661 ttgctcaatt cttttctagg cagattatct gttacgattc ctcgactaaa atcaatgacc
     6721 cattctatcg cattttgttt gcctgttggt ttcaacttaa gatttcttgt aaaaaagtaa
     6781 gctaagaaga aaacaatcaa acaggtcagt aacaccatta tacaaactgt accatcaaac
     6841 caaattggtc caatgtggaa cgttagcgag cgttcatcca atccatttca cctcttttcc
     6901 ccgaaattca cataaaatca attcatgtgc gtatctggtt ttttccaatc ggtttctaac
     6961 cacacggtat cataccacca tgaaacagaa aattcaaaac attttcatga aaattagaca
     7021 cttttttatt tcagtcaaaa acgaagcgaa tttttcttta tacacggtgc tcctgatcta
     7081 agaaaaaacc tatgataatc atgttttatc agattaaatg ctaacaaatt ttatcattaa
     7141 gtaaaagcaa aagcaccgaa tatgcgaaaa aggataatga aattatctag agaaattttt
     7201 taatgttcga tcaatttgtc gatcgatatc ttttcgtttc aaatcttccc gtttatcata
     7261 agattttttc cctttcgcca gaccaatcaa taccttggca tagccgttac gaatatagac
     7321 ttttaaagga acaacagtga ttccagtatt tttcaattca ttttcaagcc ggatgatttg
     7381 ttttttgtgt aataataact ttctcgtgcg caacggatca tgattaaaaa tatttccttg
     7441 ttcataagga ctgatatgaa cattatgcaa gaaagcttct ccgttgcgga cgcgaataaa
     7501 tccatctttt agattgatcc ggctgtttcg tatcgacttg atctcggttc cttgcaagac
     7561 catccctgct tccatcgtgt cgatgatcga ataatcatgg cgagctttct tgttttgtgc
     7621 aattaatttt ccctcgcctt ttggcatagc tgtttcctcc aactcatttt attttttctt
     7681 tttcttcttt ttaacacctt tgtaaaatgg cttcttccct tttttcttac cgaacgtttg
     7741 cttactatcg ctatatcgtt tgttcttctt gccatttctt tctgggcgtt ttcctgcttg
     7801 ttttccttcg cgttttcctg attttccatt tcgtgttttt ggccgttcca acgctgtaac
     7861 ctcttctgcg gaaacaagtt caaaatctac tgctcgttca tctggatcag ctttgactac
     7921 ttttactcgc actttctgtc cgattttaaa agtcagacca gttctttcac ctacaagagc
     7981 taaatgattt tcaacaaaat ggaagtagtc ctgtttcaat tcattgatat ggatcaatcc
     8041 ctctactgta ttcggcagtt cgataaacag gccgaatttc acaacagagc tgatgatccc
     8101 atcgaattct tcccccactt tgtccatcat gaattctgct ttcttcatgg aatctacttc
     8161 acgttctgtt tctactgcac gacgttccat cttagaacta tgatctgcaa tttcaggaag
     8221 ttccgtagcc cacgaacttt gattgctttc acttttatct tcactgtaag aacggatcag
     8281 acgatggacg attagatcgg gatagcgtcg aataggagat gtaaagtgtg tgtagtaatc
     8341 tgccgctaag ccataatgcc caaaattgtc ttctgaatag cgtgcttgtt gcatgcttct
     8401 tagcaacata gtattgataa ctgcagcttc tggtttgtct tctactcctt cgatcacatg
     8461 ctgtaaatct tttggtgtga tcgttccttt cgttcctcgg accagaatgc ccaaagctga
     8521 agcaaaatca aagaagcgct gcattttttc ttcttttggc tgttcatgga ttcggtaaat
     8581 aaacggcaga tttaatttat tataatgttc agctaccgtt tcattggccg caagcatgaa
     8641 agattcgatc aagcgttcac caattcctct gcttcttagc tggatatcat ttgggtgtcc
     8701 ttcagcatca acaaggattt ttgcttcgcg atcctcaaaa gaaatcgcgc cacgtttgat
     8761 tcgcatcgtt tcaaggattc gatgcaattc acccatttct tggaacatag gtacgagctg
     8821 agcatagcgt tgcatcgttt cttcgtcttg atcttctaag atttgattga cagctgtgta
     8881 ggtcatccgt tctgttgttt ggatcacact tgggaaaata tcatgagaga ctacttgtcc
     8941 atttggatca atctccattt cgcaactcat ggtcagcctt ggaacatggg gattcagtga
     9001 acagatacca ttcgataaac gttgcgggat catcggaatc actcgatcag tcaaatatac
     9061 acttgtccct ctttcatatg cttctctgtc caattgactg ccttgtgtca catagtaaga
     9121 aacatctgca atgtgtactc caagaaaata attaccgtta tctaattttt taaccgtaac
     9181 tgcatcatct agatcttttg catcttctcc atcaatcgta acaatgagct gatttcttaa
     9241 atctctacgt ccagttatat ccttttcgct gatttgatct ggaacttcat ctgcttcaga
     9301 caatacatca tcaggaaatt tcgtagggat accattagcc acgacaattg agaggatatc
     9361 catgccaggg tcattcttat gaccaatcac ttgcttgatt atcccttcta aactagtcgc
     9421 ataccctttt tcagggtaat gggtcagttc gatcagcaca atacttccgt caactggacg
     9481 aactccctct gctgctgcat atactttaaa ttggttgagc tttctatctt tcgggataac
     9541 aacgccgtaa agatccgttt cttgtatttc ttcttctgaa taagcaacga attcccctgc
     9601 taactgagta gtcgcccggt ctcgaatctc gactactttc ccctctgctc cgcgatccat
     9661 atctgtattt gctcgcttca tgatatcgat tgctacggtg tctccatcca ttgcaaaacc
     9721 agtcgcttct tttgggatat agatgtcatc ttcttcggga tcaatcgtta caaatccaaa
     9781 tcctcgttca tttgctcgaa atgttccttc gatcaagatt ggttttaaag gaagtttcac
     9841 ttttcctttt ttattgaaaa ctatactctg ttctctttcc atttgagcaa tcgtttgtac
     9901 tagtaattta aaatcatcgc ttttttggaa ccctaaacct tcagcgattt cttccataga
     9961 aaaactgcgt ttgcgactgc tttccatgaa aaagagaatt ttctctttta tcgtttcttt
    10021 tgtcatattt attcctcatt ccaaggtaat ttttctaaaa attccaagac gtctttttct
    10081 aattcgcgtc ttgctgtacc aactgtaatc acatgaccac tttcaggata ccatcgaagt
    10141 gtcactcgct gacggctcaa cttgcgtgca gtttcaaata cgccattagg gtcgatcatt
    10201 tcatctttcc cagcttgagc taaaaatatc ggctgttgga tatctgacaa cctactttct
    10261 acgattgctg cctgatcttg tatatccatt aattgttgtt cagctaacga gcggtaagca
    10321 actgcttttt cattgatttc tttctctgat ttacctgctg tcttcagcac ttgacgtacg
    10381 tatttttcaa agttttcagg cacgtttgtt tttacaggag acaaaggcga acagaaaaaa
    10441 ccgccgccaa tcactgattc ttcttctagt gctcgtacag caaagatgcc gcccattgat
    10501 aatcctaata cggcaatatc agaaaactgc tcagactgta aaaaatggat ggctttttta
    10561 gtgtctgccc accagctttc tgctttttct tgcaaaatat catatggctc cattgttccg
    10621 tgtcctttaa aaagcggcgc ataaactgta taatctgctt tttccaaaaa tcgtgcgagc
    10681 attcgaacat cgtttggact tcctgagtaa gcgtgaagca gcaaaaccgc tcgctttcca
    10741 tgttttgcat aaaggggttt tggtaattgt ctcactcgtc tcacctcttt cgcctcattt
    10801 taccatagtt tctttcataa cttcttctta agcgttctct ttgcaataaa aaaagccgcc
    10861 cgccgttttg cgagaggctt attcaactac ttcgaagata aatatgctag gacaaataat
    10921 aagatcatcc agatagctcc cataactgca gttgatcgct gcatgacagc ttcaaagccg
    10981 cgagcttttt gtttgccgaa tagttgatcc gcaccgccgg taaatgcact cgctgcacta
    11041 ttttgcttgc ttggctgcat cataatcgca atgaccataa caatcgataa aacgatcacg
    11101 attcccaaaa ttaaattata catatcgcag cctccttccc aaaagatatc gtctctttaa
    11161 ctttatcata gataaaagtc cgtgacaact ctatataaat ggaataacag ctgaaagttc
    11221 aaaactttca gctgttattc ttatgactga cgtaagacgt cttgatattc tggtttcttt
    11281 tcaaactctt tttttgcata agggcataaa ggaatgatct ttttcccgtc tcgtcttgct
    11341 aattctactc cagtatacac tagcttttca gctaactttt gaccacgata tgctggatct
    11401 acaaatgtat ggtcaatgat catgatgtct gggcccgcat ctgaccatgt catttctccg
    11461 atctcttgtt gttcatcatt gaataaagca agacgatttt tttcttcttt gatttccata
    11521 tgccgcactc ctcaaataac tgatattttt ttattcctac cttattttag cagtctcctg
    11581 atctataaac aattatttat cctcgaatta cttacaaaaa agaagagaaa attaattcat
    11641 cgttattctt tgttcaataa atgaaaaaat cactgtccaa taggttttcg gatctttcat
    11701 cgatgcttcc aagtgttctg cttctggaac aagcagcagt gctttatccc ctgccgtagc
    11761 attttgaatc gtgtacgctg cttctgtagg gacaaaatga tctctttcac catgaatgac
    11821 caataaaggc agatggttgc tgccaagctg tctcgtagct gatgcttgct ttaaggagta
    11881 accggcgtat ttctgggcat aatgattcgc atatctcatg accatttttt taggaaagac
    11941 agtgatttta cttaacatat atcggaattc tgcataaaca gaaacatatc cgctgtctaa
    12001 aataaatcct ttgaccgctg atggaagttt ttcaccactt gccatcatga tcgtagaagc
    12061 tcccattgat ccgccgtgta aaataatgga cgcattcggc tgttcatcta agatgagttg
    12121 gatccaggca attaaatcta gccgatctag ccatcccatt ccgatgattt ctccttcact
    12181 ttctccgtgc gcacgtaaat caggtacaag aacgttgaat cctttctctg catatctttt
    12241 acctatatga gacatgtctc ttttccctgt gcttcggtaa tcatgtacgc aaatgaccca
    12301 tttattctgc ttatttggat ggatgaacat ctgaccagct agtttcaacc cgtcataact
    12361 tttgattgtt ttgggtgttc cctgctccca aaagctttca cactcttctt ctaatttttg
    12421 tcgttttttt tctactgacg tgactgcagc agggtccatc aaacgatgac cagtgtcttc
    12481 ataccagtca tatcctctct gtactcccgc tcttactaat ttctttgcat aaaaataaga
    12541 aagaaaatgg taactgatcc ctataattat cagaataaga atgaaataaa aaataaccat
    12601 tgtacacccg ccgctttctg ccaaactata atctaactat tatccaatgt accgatccat
    12661 aggattcttt attttcggca cacgtaaatt ccatttcttt ttcattgtat catttactta
    12721 aagttctcct caatagattt acttgtaaga gagcatttac attcgcaata aaagccagta
    12781 tactaatttc gtgcattata atcaagacgc tcgtcttgga ctggttcgga aacttcccag
    12841 aataaaaaac taagtatctc taaagaaagg agattggaaa taatgcgaaa agtaattatt
    12901 gattgcgatc cagggatcga cgacacttta gctttatcat tagctgtcaa atcccctgat
    12961 atcgaagtag ttgctattac cgtagtttgt gggaatgtcc ctgcagatat aggtacgcag
    13021 aatgtgctga aatgcctgga acgctgtgat cgtttagaca ttcctgttta tcaaggaatg
    13081 gaaaaacctt tgaagcaacc tttcatcagt gcacaagaca ctcatggtat ggatggttta
    13141 ggggaaacaa atttcccgat gatcttgaga aaacaggcag aacctctcca tgccgttgat
    13201 tttttggctg attatttcaa agaaaagaca aatacatcag tcattgcttt gggtcctttg
    13261 accaatatcg cttttgcttt gaaagtcaac ccaaatattg ggaaacatat ggatcgtttt
    13321 gtctcgatgg gtggaactta caaaagccat ggtaactgtt cgccagttgc tgaatacaat
    13381 tactggtgtg atcctgaagc agcttcttat gtattcgaga atctcaaaca aacgatagaa
    13441 atggttgggt tagatgtgac aagagaaatc gtcttgacac cgactatcct cgaatactgc
    13501 tgtcaaatga atcctgaaga gggagagtat ctaaaagcga tcactcgttt ttattttgac
    13561 ttccactgga aacaagaaag aattttagga tgtgtcatca atgatccgtt agctgttgct
    13621 tattttatcg agggggccat ctgtgaaggt ttcaaaagct tcactgctgt ggaaactcaa
    13681 ggaataagtc gtggacaaac attggttgat cgttatgaat tttggcagaa acctgctaac
    13741 agtaaaatta tgacaaaagt agatactcgt ctttttttcc gaaagttttt aaccgttctt
    13801 ttaaatgcac aagaagaaac gattatgaaa gatttggaaa ggttaaagat gggctaacaa
    13861 aatgaataaa aacaaaatgg caactcgcgt acttacagtg atggctttgt gtattgggat
    13921 caattatatt ggaggaacga ttgctttatg gcttcgtttg ccaatctatc tcgattctat
    13981 cggaacgatt tttgctggtg cattgcttgg gcctgtacct ggaatgctga caggattgtc
    14041 cagcggtatt ttaagtggag tgactacaga tattttttct ctgtattatt cgccaatcca
    14101 aattctcaca ggcttgcttg ctggattgat tttgccaaga tatctcggca aagataaaaa
    14161 cagcttgaaa ctgttattac tcgctttgat tttgtcggta cctggaacga tcgtttcttc
    14221 gatcattacc gttcagttat ttggcgggat cacctcctca ggatccagta tgatcgttca
    14281 gctattacat gggttaggtt taagtcagac aacaagtgtg atcttggtac aggcaggtac
    14341 agactacctt gatcgtttgt tgtctatcgt cgttgtttca ttagtcatca ctatgttgcc
    14401 acaacgcttg atatattcaa aaagaaaaat aggctgaata aacagagctg gattagcaaa
    14461 ataatgctaa tctggctctg ttttttcatt tattaaaaaa atcttacgct aagtaggata
    14521 aaataattcc ttgatatttt tcaatcatat ccaaataatt atcgatttca acatactcat
    14581 ctacttgatg aggcaaagta acgacaccag gcccaaagac gacaaagtcg aaggaatggt
    14641 tagctttcgt aaattcggct gcatccgttg ttgctgccgc tccaactaaa ggtaatggct
    14701 ggctgaattg ttgctgaatg caatgaatca aaggagaatc tggatcagct ttgacaggta
    14761 ttttgttgta atcgatcatc aattccaaat gatagtctgt ctcttgattc aattcattga
    14821 cgattgattg cagcaatgca atgattttat catttgggta ttctggaatc gatcgaatgt
    14881 tcccttggag ctgcgcatgg cttgggatgc tgttgacttg attcccgcca ctgatcaatg
    14941 taacattatg gattgttttt cctaggacag gattctcgat tgtttcagcg aggtgattca
    15001 tttcagcatt tgctttcgta atgaattcat ttaggtgatt gattgcatta taaccttggt
    15061 ctggcataga gctatgcgct tcttttccgt gcgaggtgac tgtatagttg attgatccca
    15121 tgtgcgtata catcaaactg taattagttg gctctccgat gatcaaggca tctaagtcat
    15181 ctacatagcc tgctttcgtg agctgttccc cacctagttc acccacttct tctccgacag
    15241 tggctaataa tttgactgtt ccgttaaatg gttttcctga ctctttcagt tcgatcatgg
    15301 caatcaccat tgctgccaag ccactcttca tatctgtagc accacgtccg tacaaacggt
    15361 tcccatggat ttctgctgca aagggtgcat aggtccaaga cgattcatca ccagccgcca
    15421 cgacatccat atgcccagat aggcctaaca cttttcctga ttggcccttt tggtaacgag
    15481 caatcaaatt atctctacca tctctgtaag aaacaatctc tcctgttatg tcatgctttg
    15541 ccaaaagctt attcaaatag gctgctactt caccttcgtt tccattcact gatttaattc
    15601 ggatgatctc ttgtaaaatc gcgatttttt cttctttttt catctaatcc tctcccttgc
    15661 catttctttt tattgtattt gagtgtgctc gaattttcaa ataggagcaa ccaaagtaaa
    15721 aatgaagaaa gagagccact ttatgaaaaa tatgctgcta cttccctcct attttattta
    15781 tacgagagga cttgctcata gattcctatt ctactgttct atccttaaaa gctcttatat
    15841 gatgctagtt attgttagct ttcctttttc aaggatgatc ttttcttgta ccattctttg
    15901 taaacatcat atacagaagc tagttgcaaa aaaaactctc tacctgctga tat
//
LOCUS       DARBET010000059        15899 bp    DNA     linear   BCT 07-NOV-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4027
            SAMEA114569841-rid20112183.denovo.061, whole genome shotgun
            sequence.
ACCESSION   DARBET010000059 DARBET010000000
VERSION     DARBET010000059.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA114569841
            Sequence Read Archive: ERR12203666
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 15899)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15899)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001972618.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001972618.1
            Genome Coverage       :: 132x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/07/2023 13:18:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,041
            CDSs (total)                      :: 2,975
            Genes (coding)                    :: 2,908
            CDSs (with protein)               :: 2,908
            Genes (RNA)                       :: 66
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 67
            CDSs (without protein)            :: 67
            Pseudo Genes (ambiguous residues) :: 0 of 67
            Pseudo Genes (frameshifted)       :: 26 of 67
            Pseudo Genes (incomplete)         :: 28 of 67
            Pseudo Genes (internal stop)      :: 20 of 67
            Pseudo Genes (multiple problems)  :: 6 of 67
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15899
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA114569841-rid20112183.denovo.061"
                     /isolate="VRE4027"
                     /db_xref="taxon:1352"
                     /country="Denmark"
     gene            138..1475
                     /locus_tag="SCJ86_002070"
     CDS             138..1475
                     /locus_tag="SCJ86_002070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320632.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="citrate transporter"
                     /protein_id="HEG1169602.1"
                     /translation="MSDIIIGILLVISFFFMVWYCVKGYNLMVGFAIMATVWMGLALV
                     GNTFSPNPAMEGQGVIDVLTHIYTTGPAEYAKSILVNVFFGAFFGRVLVDSGIAATLI
                     RKVVELGGDKPRITMSLLCIVTAVIFMSMTGIGPVISIAVIVLPILMSLGISVPVALF
                     SFMGSIMAGIFANIVNFKQYQTIYAGFNPAAESYTYNDYFQIGMIGMIVSLVVVLTVA
                     NISMNKKKRYAMAANVPAEGGDAPMISWLAVLLPVLGVVLLDLPIILGFILAGIWALL
                     FTGKLRGGYKEICRQFAKLFTDGAVDVAPMVGFLMTLAMFNNSAAYASPYFSAIFGDW
                     IPQSPLVLAIVFAILTPLGFFRGPMNLVGSGSAILAVVLAVNPTMSPAFLFPLFAITT
                     IAPQHLDITQSWVAWGLGYTKVTSREYMKKSIPTGWIIGAILCLITFLLYGNA"
     gene            1523..3652
                     /locus_tag="SCJ86_002071"
     CDS             1523..3652
                     /locus_tag="SCJ86_002071"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydantoinase/oxoprolinase family protein"
                     /protein_id="HEG1169603.1"
                     /translation="MKRFVRMGIDVGGTHTKAVAIDNATHEIIGKSSVKTTHDDVRGV
                     AAGVVQSFQNCLLENNISPEDVVFVAHSTTQATNALIEGDVAKVGVIGMAKGGLEGFL
                     AKRQTRLNDIDLGNKKKIEIVNAFLPVKHLNVDRVSETISSLERERAEVLVSSMAFGV
                     DNGEPERVVYEAASVKSIPTTMASDITKLYGLTRRTRTAAINASILPKMLDTATSTED
                     SVREAGVNVSLMIMRGDGGVMEINEMKKRPVLTMLSGPAASVMGSLMYLRASNGVYFE
                     VGGTTTNIGVIKNGRPAIDYSIVGGHPTYISSLDVRVLGVAGGSMVRANQSGIIDVGP
                     RSAHIAGLDYAVFTETEKIKGPKVEFFSPKEGDPADYVKVVMEDGEEVTITNTCAANV
                     LGLVQEEHFSYGNVPSARKAIQALADYCHTTVEDIAEQIMEKSYAKIEPVILELADKY
                     HLEKDQISLVGVGGGAASLITYFSNKMGVKYSIPENAEVISSIGVALAMVRDVVERII
                     PSPSKEDIRSLKNEAMNKAIESGATPESIEVHVEIDPQTSKVTAIATGSTEVKATDLT
                     KEITTEEALELAAEDMRLNKNEVCLLENTPFFYVCGEQNRSKNAGSLRIIDQKGFIKV
                     QRGHASCMKTTAANYMTAVEQLWEDMAVYQTELIARPEFYLCLGARVSDFTATDLEQL
                     QLLMDLEVSTMEPEEEVIVVAGNIKQT"
     gene            3627..4316
                     /locus_tag="SCJ86_002072"
     CDS             3627..4316
                     /locus_tag="SCJ86_002072"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEG1169604.1"
                     /translation="MPEISNKHKLEELLNELQQIPEEIWGFYQFRRDLFWKKIPLSKQ
                     KILIQQSIDCGIETACSIKKKYPFADVGEICEQMAVPIVSCESEQINERITFATYAED
                     EGIRLMTEPLEKLKCSGLASISKETAQALIIGHELFHHIEASVKGIYTQSEKIVLWRL
                     PFYTHQSNIRALSEIAAMSFSKEMNQSDFSPYVLEAVLLWPYNEIRSQGILEEIKKIE
                     KRCTEYDLDYK"
     gene            complement(4724..5410)
                     /locus_tag="SCJ86_002073"
                     /pseudo
     CDS             complement(4724..5410)
                     /locus_tag="SCJ86_002073"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374310.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
     gene            complement(5546..5740)
                     /locus_tag="SCJ86_002074"
     CDS             complement(5546..5740)
                     /locus_tag="SCJ86_002074"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEG1169605.1"
                     /translation="MKRKKQVIPMYVVKVMHGYIDKTGCRTREKNLDNLLIFKDKKES
                     EAFAKRIGGRVKPIQEVRPD"
     gene            complement(5790..6230)
                     /locus_tag="SCJ86_002075"
     CDS             complement(5790..6230)
                     /locus_tag="SCJ86_002075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331188.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA thioesterase"
                     /protein_id="HEG1169606.1"
                     /translation="MEKFTGYVREPFYYETDQMGIIHHSNYIRWFEEARVALLVHLGF
                     PYKKIEESGIIIPVLEVNCSYKEMIHYGEKVSIHPTIQKYTGTRLDFSYEIVGTEDKK
                     LKTTGSSKHCFLLSDSQRLVKLSKVNPELDQLFQAYASLDDVNS"
     gene            complement(6234..7079)
                     /locus_tag="SCJ86_002076"
     CDS             complement(6234..7079)
                     /locus_tag="SCJ86_002076"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008264125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-hydrate dehydratase"
                     /protein_id="HEG1169607.1"
                     /translation="MLINEQLVKRVITPRPKISHKGTFGRIVLIGGNEQYGGAIIMSA
                     LAAVHSGAGLTTVVTDEKNRSALHSHLPEAMIVVWTDTDQFLSVIKQADVLLIGPGLG
                     TSSKSLERFKQVLENQSKEQWLVIDGSALSLLAANHLLLPYPEQTVLTPHQMEWQRLS
                     SLPIQEQRDKENKKKQQEIGAVLVLKSHETKIYGHQHFYTNPLGSAAMATGGMGDTLA
                     GMITGFLAQFPQNDQTIAAAIYLHSFIGDQLAKEQYIVLPTQISEALPKWMNYFCKKS
                     MENEA"
     gene            7228..8646
                     /gene="pepV"
                     /locus_tag="SCJ86_002077"
     CDS             7228..8646
                     /gene="pepV"
                     /locus_tag="SCJ86_002077"
                     /EC_number="3.4.13.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723407.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dipeptidase PepV"
                     /protein_id="HEG1169608.1"
                     /translation="MTIDWQKEVEARKEDLLEDLKNLLRVNSERDDSKVTPDAPFGPG
                     PRDALKHMLAYGERDGFKVKNVDNYAGHIDLGEGDETLGIFGHMDVVPAGDGWDTDPY
                     EPVIKDGKIFARGSSDDKGPSMAAYYAMKIIKELDLKLSKKVRFVVGSDEESGWGDMA
                     YYFEHEEEPDFGFSPDAEFPIINGEKGNVSLALRFKGDNAGDYVLKSFVSGLRENMVP
                     GTATAALQVPSADAAIAMEEAFYQFIEANPVSGTIEADNTYVKIELVGKGAHGASPQS
                     GINAGSFLALFLDNYEFLGSAKQFIHVAAAYVHEDFYGEKLGVAYEDEKMGKLTMNAG
                     LFAFEENGTEEANFINMNFRFPKGVTVDGLQSDIEQTVGQEGATVTRGARVMEPHYVP
                     MDDPLVATLLQVYEDHTGEKGYEQIIGGGTYGRLLKRGVAYGAMFPGYTDTMHQANEF
                     MSLDDLFRATAIYADAIYRLAK"
     gene            complement(8703..9650)
                     /locus_tag="SCJ86_002078"
     CDS             complement(8703..9650)
                     /locus_tag="SCJ86_002078"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HEG1169609.1"
                     /translation="MEQQEKIAVLIPCYNEEATISTVIADFKRELPEADIYVYDNNST
                     DRTYELAVQGGAIVKKEPRQGKGNVIRQMFFDIDADYYLMVDGDDTYPAEAVHGLLEK
                     LRSGEADMVIGDRLSNGTYFDENKRPFHDFGNNLVRNTITRMYKTKILDVMTGYRGFN
                     RIFVKSFPIMSSGFQIETELTIHALDKKFKFVEIPIDYRDRPEGSESKLNTFSDGFKV
                     IMMIIKMFKDYKPLLFFGIWTIFFFLFGLVTGIPVIREYMLTSFITRIPSAILSTGLM
                     TLALLSLVTGLILDTVVTNAKKEYELNLYHVYHEYHSRK"
     gene            complement(9873..10223)
                     /locus_tag="SCJ86_002079"
     CDS             complement(9873..10223)
                     /locus_tag="SCJ86_002079"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PepSY domain-containing protein"
                     /protein_id="HEG1169610.1"
                     /translation="MYEESELSYFKGGLAVGIGLGLLSGVASTIWYHRKKVLSADQVL
                     ENIKSSFLKEGAIEGSWISFEKEPIRKFAVRSSAYRGGLSRIEDGETVYYEFLADAHT
                     GTVLEISRKKGATD"
     gene            10423..11502
                     /gene="pepA"
                     /locus_tag="SCJ86_002080"
     CDS             10423..11502
                     /gene="pepA"
                     /locus_tag="SCJ86_002080"
                     /EC_number="3.4.11.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016632088.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamyl aminopeptidase"
                     /protein_id="HEG1169611.1"
                     /translation="MEEKTFQRIKELTELQGTSGFEQDVRAYMESHMEPLVDELQLDG
                     LGGIFGLRHHEEADAPRVMVAAHMDEVGFMLTQIQENGLFKVVPLGGWNPYVVSAQRF
                     TLKTSTGKNYPCISSSVPPHLLRGTSGQKSVEVTDILFDAGFESREEAMSYGVLPGDT
                     IVPYAETIKTANGKNIISKSWDNRYGCTMVLEALEALQNETLGHTLIAGANVQEEVGL
                     RGSKASVNKFKPDLFFAVDCSAADDTVTKNGTFGHLGEGTLMRIQDPGLIMLPRLREY
                     LLDIAETNNIPYQYFVSKGGTDAGAAHTQNEGIPSTVIGVVGRYIHTHQTMFSIRDFE
                     AAREMLIQTLKGLDKSTVNTIVYGK"
     gene            11646..11966
                     /locus_tag="SCJ86_002081"
     CDS             11646..11966
                     /locus_tag="SCJ86_002081"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374322.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin family protein"
                     /protein_id="HEG1169612.1"
                     /translation="MIIPNSLEELAGYVETGKSVFFFTAGWCGDCRFIKPQMPEIEND
                     FSDWQFIEVDRDKYIDVAAEWNIFGIPSFVVIQDGKELGRLVNKDRKTKQEIESFLES
                     VTKG"
     gene            11988..12452
                     /locus_tag="SCJ86_002082"
     CDS             11988..12452
                     /locus_tag="SCJ86_002082"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein"
                     /protein_id="HEG1169613.1"
                     /translation="MNQEYKNILVGIDGSEQALAAFKKAVEVARRNNGKVYVANVIDQ
                     QFYNFMGYAPIDQNLVDQQTETAKEMIEECKEYGKSVDYTNIEGIIAYGSVRESMAVK
                     LPKKYEIDLIMVGQSGLNAVERFMTGSVASYVIRHSPCDVLIVSDEEEQPSN"
     gene            12657..13262
                     /locus_tag="SCJ86_002083"
     CDS             12657..13262
                     /locus_tag="SCJ86_002083"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4479 domain-containing protein"
                     /protein_id="HEG1169614.1"
                     /translation="MIFAYNKAHVGDTLMVIIADDKGTENTVERKWNVARIKDEQGQV
                     VAWNFFHISDHLTIEGNGQVSLTDEELAKLNRLIKEAGFEEQLEADHEPKIVVGYVKT
                     CVPHPDSDHLSITETEVDNGTVLQIVCGAPNIEAGQKVVVAKPGAMMPDGMMIWPGTL
                     RGVDSYGMICSARELHLPNAPAKKGILVLPEDAVVGEAFPK"
     gene            complement(13301..14590)
                     /locus_tag="SCJ86_002084"
     CDS             complement(13301..14590)
                     /locus_tag="SCJ86_002084"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628497.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trypsin-like peptidase domain-containing
                     protein"
                     /protein_id="HEG1169615.1"
                     /translation="MDRKNVTPKMKKSKNNSLWRKLGLGLVGGIVGGLLTAGIFYAAM
                     GTGNNSSTATSGNQNSAGETVVENVKVNVDSDITKAVDKVQDAVVSVINLQSQNQSSG
                     FGGLFGQQEESSSSDDSNLEAYSEGSGVIYKKDGNTAYVVTNNHVVDGQQGLEVLMKD
                     GTKVKAELVGTDAYSDLAVLKINSDKVETVASFGDSSALKVGEPAIAIGSPLGSEYAN
                     SVTSGIISSLNRQVTSTNESNETVNINAIQTDAAINPGNSGGPLVNIEGQVIGINSSK
                     IASTSESTSNVSVEGMGFAIPSNDVVNIINQLEKDGKVTRPALGITMVDLSAVSTQQQ
                     EQILKIPSSVTNGVIVTNVQTATPAEKAGLKQYDVITKIDDKEVSSGVELQSALYKKK
                     VGDSVKITYYEGSKEKTTTVELTIDQSALKQNQNSEN"
     gene            15018..15497
                     /gene="rlmH"
                     /locus_tag="SCJ86_002085"
     CDS             15018..15497
                     /gene="rlmH"
                     /locus_tag="SCJ86_002085"
                     /EC_number="2.1.1.177"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019722872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA
                     (pseudouridine(1915)-N(3))-methyltransferase RlmH"
                     /protein_id="HEG1169616.1"
                     /translation="MNIKIISVGKLKEKYLIQGINEYVKRLNAYAKIELIEVPDEKAP
                     ENLSEAQMRQVKEKEGERILAKIKEQEYVYALAIEGKNPTSESFAKQIDQLGIQGKSH
                     LVFVIGGSLGLSEAVMKRSNAQISFGKMTYPHQLMRLILVEQIYRAFRINAGAPYHK"
     gene            15700..15899
                     /locus_tag="SCJ86_002086"
                     /pseudo
     CDS             15700..15899
                     /locus_tag="SCJ86_002086"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336126.1"
                     /note="frameshifted; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="PD-(D/E)XK nuclease family protein"
BASE COUNT         5009 a         2933 c         3245 g         4712 t
ORIGIN      
        1 cataattaag gttttttaat tgaaaactga acatttatgg tcacccttta atgaaagcgc
       61 taactacata agttagaatg agtttgactg atatattagt ttgcttatca gaaaaacaaa
      121 atggacggag gaataacatg agtgatatta ttattggtat tttgttagtt atctcattct
      181 ttttcatggt ctggtactgt gtaaaaggct acaacttgat ggtcggcttt gccatcatgg
      241 ctactgtatg gatgggatta gcgctagtcg gaaacacgtt ttcaccaaac ccggcgatgg
      301 aagggcaagg agtaattgat gtgttgacgc atatctatac gacggggcca gctgagtacg
      361 ccaaatcgat cttggtaaat gtcttctttg gtgccttttt cgggcgtgtt ctagtagata
      421 gcggaatcgc agctaccctg attcgaaagg tagttgaact tggaggggac aagccaagaa
      481 tcacgatgag cttactatgt attgtaaccg ctgttatatt catgtcgatg acaggaatcg
      541 gaccagtgat ttctattgca gttattgtgt tgccaatctt gatgtcatta ggtatttcag
      601 taccggttgc actgttctca tttatgggct cgattatggc tggaatcttt gctaatatcg
      661 tcaatttcaa acaatatcaa acgatttatg ctggtttcaa tccagcggct gaaagctata
      721 cttataatga ctatttccaa attggtatga ttgggatgat tgtaagctta gttgtggtac
      781 taacggtcgc taatatatca atgaacaaaa agaaacgcta tgcaatggca gcaaacgtac
      841 cagctgaagg tggcgacgca ccaatgatct catggttagc cgtattatta ccagttttag
      901 gggtcgttct tttagatctt ccaatcatct taggttttat tctagcaggt atctgggcat
      961 tgctgtttac tggaaaattg cgcggaggtt ataaagaaat ctgtcgtcag tttgcgaaat
     1021 tatttacaga tggcgcagta gatgttgcgc caatggttgg tttcttgatg accttagcga
     1081 tgttcaataa ttcagctgcg tatgcttctc cttatttttc agctattttt ggagattgga
     1141 taccgcaatc accattagtc ttggcaatcg tatttgctat attgacacca ctaggatttt
     1201 tccgcggtcc aatgaatcta gtcggttcag gctcagctat tttagctgtc gtattagctg
     1261 ttaatccaac gatgtcgcca gcctttttgt tcccgttatt tgcaatcaca acgattgctc
     1321 ctcagcattt agatattaca caatcttggg ttgcttgggg attaggttat acaaaagtaa
     1381 cttctcgaga atatatgaag aaatccatcc caactggttg gatcatcgga gcgatcttat
     1441 gcctgatcac gttcttattg tacgggaacg cctagtaaac aaaacgtcga taaaaacgat
     1501 tagatgaatg gaggaaaaga aaatgaaacg ttttgtgcga atgggaattg atgttggcgg
     1561 tacacatacg aaagcagtcg caatcgataa tgcgacgcac gaaatcatcg gaaaatcttc
     1621 agtgaaaaca acccacgatg atgtccgtgg agtagccgca ggtgtcgttc agtcttttca
     1681 aaattgtttg ctggaaaata atatcagtcc agaagatgtt gtgtttgttg cacattctac
     1741 cacacaagcg actaatgcac tgattgaagg agacgtagca aaagtcggcg taatcgggat
     1801 ggcaaaaggt ggattagaag gttttttagc aaaacgacaa actagattga acgatatcga
     1861 tttagggaat aagaaaaaaa tagagattgt caatgcattc ttacctgtca aacacttaaa
     1921 tgtagaccgt gtttcagaaa cgatttcttc tttagaaaga gaaagagcgg aggtcttggt
     1981 ttcttctatg gcatttggag tagataatgg ggaaccagaa cgagttgttt atgaagcagc
     2041 atcggtaaaa tcgatcccta caacgatggc ttctgacatc acaaaattat atggattgac
     2101 aagaagaact cggacagcag caatcaatgc atctatttta ccaaaaatgc tagatacggc
     2161 aacctctaca gaagattccg tacgagaagc aggggtcaat gtttctttga tgattatgcg
     2221 cggagacggc ggagtaatgg aaatcaatga aatgaaaaaa cgacctgttt taaccatgct
     2281 ttccggtcca gcagcatcag tcatgggctc tttgatgtac ttgagagcgt ctaatggtgt
     2341 ttattttgaa gtaggcggaa caacaaccaa tatcggggtc attaaaaatg gacgtccagc
     2401 aatcgattat tcaattgtag gtggccaccc aacatatatc tcttcattag atgttcgggt
     2461 tctaggtgtg gcaggcggtt cgatggtacg ggctaatcag tcaggaatca tcgatgtcgg
     2521 tccacgttct gcacatattg ccggtctaga ttatgcagta tttacggaga cagaaaaaat
     2581 caaaggtcct aaggttgaat ttttctctcc taaagaagga gaccctgctg attacgtcaa
     2641 ggtagtgatg gaagacggag aagaagtgac tattacaaat acatgtgcag ccaatgtcct
     2701 tggattagtg caagaagaac atttttctta tggaaatgtc ccatctgcac gaaaagccat
     2761 ccaagcatta gctgattatt gtcatacgac ggttgaagac atcgcagaac agatcatgga
     2821 aaaatcctat gctaagattg aaccggtgat tttagaattg gcagacaagt atcacttgga
     2881 gaaagatcag atttctcttg taggtgtagg gggcggcgct gcctcattga ttacttactt
     2941 tagtaataaa atgggtgtga aatattcgat tcctgaaaac gccgaagtga tttcttctat
     3001 tggtgtagca cttgcaatgg tccgagatgt tgtagaacgt atcatccctt cgccaagtaa
     3061 agaagatata cgatcgttga aaaatgaagc gatgaataaa gcgattgaat caggcgctac
     3121 accagaatcg atcgaagtcc acgtagaaat cgatccacag acttcaaaag taacggctat
     3181 cgctactggt tctacggaag tgaaagcaac cgatctaacc aaggagatca caacagaaga
     3241 agcactagag cttgctgcag aggatatgcg attaaataaa aatgaagttt gcttattgga
     3301 aaatacacct ttcttttatg tatgcggcga acagaatcgt tcaaaaaatg ctggaagtct
     3361 tcggatcatc gatcaaaaag gatttatcaa ggtccaacgt ggacatgctt catgtatgaa
     3421 aacaactgca gccaattata tgacagctgt cgaacaactt tgggaagata tggcagttta
     3481 tcaaactgaa ttgattgccc gaccagaatt ttatctctgc ttaggagcaa gagtctctga
     3541 ttttacagcg acggatctgg aacaactaca gcttttgatg gatctggaag tatctactat
     3601 ggaaccggag gaagaagtga tcgtcgttgc cggaaatatc aaacaaacat aagttagaag
     3661 agttactaaa cgaattacaa cagatccccg aagaaatttg gggattctac cagtttcggc
     3721 gagatttatt ttggaaaaag attcctttgt ccaagcagaa aattcttata caacaatcaa
     3781 tcgattgtgg tatcgaaacg gcatgtagca tcaaaaaaaa gtaccctttt gccgatgtag
     3841 gagaaatttg tgaacaaatg gctgttccaa tcgtttcctg tgaatcagag caaataaacg
     3901 aacgaatcac ttttgcaact tatgcagaag atgaagggat tcgattgatg acggagccgt
     3961 tagaaaaatt aaaatgttca ggactagctt cgatctctaa agaaacagcg caagctttga
     4021 ttattggaca tgaactattt caccacatcg aagcttctgt taagggaatt tatacgcaaa
     4081 gcgaaaaaat tgtgctgtgg cggttgcctt tttatacaca tcaatcaaac attcgtgcat
     4141 tgagcgagat agcagcgatg agtttttcaa aagagatgaa tcaaagcgat ttttcgcctt
     4201 atgtattaga ggcagtctta ctttggccat ataatgaaat tcgtagccaa gggatcttag
     4261 aagaaataaa aaaaatagaa aaaagatgta cggagtatga tttagattac aaataaagag
     4321 actgtgacaa aagtcgtgtc acagtccttt ttttccgaat aaacggtgtt ctcctgtcaa
     4381 ctcctcggtc acaagtcaaa atattcaaca aacatgagaa gctgtttttt gaaattgttc
     4441 cttgtgactc ggagatgaac ggcaggctca cgacctcttg gaaaacggtg ttcaaaagct
     4501 tgcatctgcg gtaataagtc cagccaagtt aaaaacataa gaagctattt ttacttggct
     4561 gttcttatta cttgatgcag ggcagctttt tcacaacctc tcaataaacg gtgtgcaatc
     4621 agctgaccct cggtattagc tcaaaaaaaa cggcaaaata tgaggaacta ttttgccgtt
     4681 tctttctcta atactcaggt caaaacgctg attccaaaac ctcttatttg atgggtctgt
     4741 cctgaatatc ttcaaaataa tcggggtaat tatgaatcac gtattcttgc tcctcgataa
     4801 tcatatgtaa atgcatcgtg caaagaaatc cgacttcatc aaagttatga tcaatcattg
     4861 attgaagcat tgtctgatgt tcatctaata ctcgacccca gtctaattct gaaatcgtca
     4921 aacgtaacca tctgaaacgg ttcaagtgcg tactataact ttctagccaa tcccaaattt
     4981 ctcgttttcc agcaatttca taacaagttc ggtgaaaatc agtatccaaa tcaaaaaaag
     5041 cttttttatt ttgagcgatt actgcctgat cggtttgttc tagtattttt tctagtttag
     5101 cttgatctta cttggtcatt ttcgcactgc attcttgcag gatcggtttt tccaactttt
     5161 cccgcgtata acgggctaat gaagcagatc ggatatcgat ttttgaaaca taagtgccgg
     5221 actgtggaat ggtttttaca agctcttgat tgcgcagttg gatcagtgct tctcgaatcg
     5281 gtgtacgccc gatagctagt tgttcttcta acactttatc ggagatttta cttccaggtg
     5341 ctaactcgga ataaatgatt tgtttacgta tagattcata agcttgctgt tgcaaattat
     5401 tgataaccat ttaaccttcc tttctatgca gatataatct attataacat aaatcgctta
     5461 caaatcgtgt gggcataaaa acacagtctg caaaaaaagc cctgataggc atgcctatta
     5521 gagcctcgaa aagaaaaata ttgaatcaat ctggtcgtac ctcttggatc ggtttgacac
     5581 gaccgccgat tcttttagca aaagcttcgg attctttttt gtctttgaaa atgagcagat
     5641 tgtctaagtt tttttcccga gtgcgacagc cagttttatc gatgtaaccg tgcattactt
     5701 taacgacata catggggatc acctgctttt ttcttttcat tatacgaact actcgacaca
     5761 atacaagaaa aatgcttaga cggtctttat taagaattta catcatccaa agaagcgtac
     5821 gcttggaata actgatctaa ctccggattg actttgctca gcttcactaa tcgttgcgaa
     5881 tcagaaagta aaaaacagtg tttactactg ccagtagttt ttaatttttt gtcttcagtt
     5941 cccacaattt cgtagctaaa atccaaccgt gtccccgtat acttttggat agttggatgg
     6001 atgctcactt tttctccgta atggatcatt tctttatagc tgcaatttac ttccagaacg
     6061 gggatgatga tcccagattc ttcgattttt ttatagggaa accctagatg taccaataag
     6121 gctacccgag cctcttcaaa ccagcggata taattgctgt gatgaataat ccccatttga
     6181 tctgtttcat aataaaaagg ttctcgcaca taacctgtaa atttctccat gactcatgcc
     6241 tcgttttcca ttgatttttt acaaaaataa ttcatccatt tcggaagtgc ttcgctgatt
     6301 tgtgtcggca aaacaatata ttgttctttt gccagctggt ctccaatgaa actatgaaga
     6361 taaatagctg ctgcaatcgt ttggtcattt tgaggaaact gggctaaaaa tcctgtgatc
     6421 atcccagcta aagtgtcacc catgccgccc gttgccatag cagcagaacc taaaggattg
     6481 gtgtagaagt gctggtgacc ataaattttt gtctcatgac ttttcaaaac gagcacagca
     6541 ccgatttcct gttgcttctt tttgttttct ttatcccgtt gttcttgtat cggtagagaa
     6601 ctcaaccgtt gccactccat ttgatgaggt gtaagtacag tttgttcggg atagggtagc
     6661 aaaagatgat tagcagcaag taaacttaaa gcagagccat caatcaccag ccattgttct
     6721 tttgattggt tttctagtac ttgtttgaaa cgttccaaac tcttagaaga agtacccaat
     6781 ccgggaccaa tcaagagtac atctgcttgc tttattacgg aaagaaactg gtcagtatcc
     6841 gtccaaacta caatcattgc ttctggcaga tgactgtgaa gagcactccg atttttttca
     6901 tcagttacta cagtcgtgag acccgctccg ctgtgtacgg cagctaaagc actcataatg
     6961 attgctccgc catattgttc attgccgcca atcaaaacga tacgtccaaa tgttccttta
     7021 tgtgaaattt ttggacgagg tgtgatgact cttttcacta gttgttcgtt tatcagcatc
     7081 taaaaacctc cttttactca ttatagcgta tatttttttt agagaaaaac agataagaaa
     7141 aggaattgtc agattttttg aactattttt ttctgtaatt tgtggtatca tgaacacgaa
     7201 gacttaaata agtggaggat taactttatg acaattgatt ggcaaaaaga agtagaagca
     7261 cgtaaagaag acttgcttga agacttgaaa aatcttttac gtgtcaacag cgagcgcgat
     7321 gactcaaaag taacaccaga tgcaccattt ggacctggac ctcgcgacgc attgaaacat
     7381 atgttagctt atggcgaacg tgatggattt aaagtaaaaa atgtcgataa ctatgcaggg
     7441 catattgatc ttggcgaagg agacgaaacg ctagggatat ttggacacat ggatgtcgtt
     7501 cctgcaggag atggctggga cacagatcct tatgaaccag taatcaagga tggaaagatc
     7561 tttgcacgcg gatcaagcga tgacaaaggt ccaagtatgg cagcttacta tgcgatgaag
     7621 atcattaaag aacttgactt gaaactatca aaaaaagttc gcttcgtagt cggcagcgat
     7681 gaagaaagcg gctggggcga tatggcttat tatttcgaac atgaagaaga accagatttt
     7741 ggtttctcac cagatgctga attcccaatc atcaatggtg aaaaaggaaa tgtttcttta
     7801 gctttacgct tcaaaggcga caatgcagga gattatgtct tgaaatcttt tgtttccgga
     7861 cttcgcgaaa atatggtgcc aggaacagca actgcagcac ttcaagtacc aagtgcagat
     7921 gcagctatcg cgatggaaga agcattttac caattcatcg aagcaaatcc agtaagcgga
     7981 acgattgaag cggacaatac gtatgtaaaa atcgaattag ttggtaaagg cgcacatggt
     8041 gcaagcccac aatctggtat caatgcaggt tctttcttgg cattgttctt agataactat
     8101 gaattcctag gatctgctaa acaatttatc catgtagcag ctgcttatgt ccatgaagat
     8161 ttttatggag aaaaactagg agtcgcttat gaagatgaaa aaatgggcaa attgacaatg
     8221 aatgctggac tatttgcttt tgaagaaaat ggtacagagg aagcgaactt catcaatatg
     8281 aatttccgtt ttccaaaagg tgtaacagta gacggattgc aaagtgacat cgaacaaaca
     8341 gtggggcaag aaggcgcaac agttactcga ggtgctcgcg taatggaacc acattacgta
     8401 ccaatggatg atccattggt ggctacatta ttgcaagtct atgaagacca cactggagaa
     8461 aaaggctacg aacaaatcat cggtggcgga acttacggac gtttgttgaa acgtggcgtt
     8521 gcttatggag cgatgttccc aggatatact gatacaatgc atcaagcaaa cgaatttatg
     8581 agtcttgatg acttgttcag agcaacagca atctatgcag atgccatcta tcgtttggcg
     8641 aaataattaa ttaaaaagag gatcaagaat tgcaaattat ttttgcagtc ttgatccttt
     8701 ttttactttc tactgtggta ttcatgataa acatgatata gattcaattc atattctttc
     8761 ttagcgttcg tgacgactgt atccaaaatc aaccctgtta ctaatgaaag aagtgctaaa
     8821 gtcatcaatc cagtacttaa aatcgcagaa gggatgcgtg taataaagct tgtcagcata
     8881 tattcgcgaa tcacaggaat ccccgtgacc aatccaaata aaaagaagaa gatggtccaa
     8941 atcccaaaga aaagcaaagg cttataatct ttaaacattt taataatcat catgatcact
     9001 ttgaatccgt cagagaatgt attcaatttt gattcgctgc cttcaggacg atcacgataa
     9061 tcgattggga tttcaacgaa cttgaatttt ttatctaaag catggatcgt caattccgtt
     9121 tcgatttgaa aaccagaact cataatagga aaacttttga caaagatacg attgaatcct
     9181 cgatagccgg tcatgacatc aagaattttt gttttataca ttctagtaat tgtatttcta
     9241 acaaggttat ttccaaaatc gtggaaaggt ctcttattct catcaaagta ggttccattt
     9301 gataggcgat caccaatgac catatctgct tcaccagaac gtaatttctc gagcaaacca
     9361 tgtacagctt ctgcaggata agtatcatcg ccatctacca ttaaataata gtcggcatcg
     9421 atatcaaaga acatttggcg gatcacattt ccttttcctt gacgaggttc tttttttacg
     9481 attgctccac cttgaacggc caattcgtat gttcgatcgg tcgaattatt atcatagaca
     9541 taaatatctg cttctggtaa ttctcgttta aagtctgcaa tgactgttga aattgttgct
     9601 tcctcattgt aacaaggaat cagaacagca attttttctt gttgttccat ttttcactct
     9661 ccttaactat agaaaacaaa agctaaatta aaaatacctc ataatcaaag gaaatgctag
     9721 tttttcccgc taaatatcat ttatttaaaa agaaaatact ggaatatatg tttggaaagc
     9781 tctatctaaa aaaccaccca ctacaaaaga aggacatgat acctctcttt tgaatagtgg
     9841 gtgcgtattc gttctctcac ttttttgacg tattaatctg ttgcaccttt ttttcttgaa
     9901 atttctaata cggttcctgt atgagcatct gctaaaaatt cataataaac agtttcgcca
     9961 tcttctattc gagaaagacc tccccgataa gcactcgacc gaacggcgaa tttcctaata
    10021 ggttcctttt caaaagagat ccaagaacct tcgattgcac cttcttttag aaaagatgac
    10081 ttgatatttt caagtacttg gtctgctgac aatacttttt tgcgatgata ccaaatcgtt
    10141 gaagctaccc cgctcaataa tcctagaccg attccaactg ctaacccgcc tttaaaatag
    10201 cttagttcgc tttcttcata catgtggaca tacctccttc ttctcacttg ttactccttg
    10261 tgcttatttt accacactct catattttct ttgaaataaa taaccgctac cagaaaaaat
    10321 aagctataat aaatacgatc ggtctgcaga aaaaatcata ttttatccgc agatcaatat
    10381 aagtctgaga ggagtttagt ctgaaattta gacgaatagc atatggaaga aaaaacattt
    10441 caacgaatca aagagttaac agaattacaa ggaacaagcg gttttgaaca agatgttcgc
    10501 gcttatatgg aaagtcatat ggaaccgctg gtagatgagt tacagttaga tggtttgggc
    10561 ggcatttttg gtctgcgcca tcatgaagaa gcggatgcac cccgtgtaat ggtcgcagca
    10621 catatggatg aagtcggatt catgctgaca caaatccaag aaaacgggct ttttaaagtc
    10681 gtacctctag gcggctggaa tccttacgtt gtttctgctc agcgttttac acttaaaacc
    10741 tctactggta agaattatcc atgtatttct tcttccgttc ccccacattt attgcgagga
    10801 acaagcggac aaaaatcagt agaagtaacc gatattttgt tcgatgctgg gtttgaatca
    10861 agagaagagg caatgagtta cggtgtcctt ccaggagaca caatcgttcc ttacgctgaa
    10921 acgatcaaaa cagccaacgg aaagaatatt atcagtaaat catgggacaa ccgctatggc
    10981 tgtacaatgg tattagaagc tttagaagcg ttgcaaaatg aaacattagg tcatacattg
    11041 attgcaggcg cgaatgtcca ggaagaagta ggcttgcgtg gttctaaagc ttctgtcaat
    11101 aaattcaagc cagatctgtt ctttgcagta gactgctctg cagcagatga tacagtgacg
    11161 aaaaatggca cattcggaca tttaggagaa ggtacattga tgcgtatcca agatccaggt
    11221 ttgatcatgc ttcctcgttt gcgcgagtac ttgctggata tcgctgaaac aaacaatatc
    11281 ccttatcaat actttgtttc aaaaggcggt acagatgcag gtgctgctca tacacaaaac
    11341 gaaggaatcc caagtacagt gattggcgta gtgggacgtt atatccatac gcatcaaacg
    11401 atgttcagta ttcgcgattt tgaagctgca cgagaaatgt tgattcaaac actgaaaggt
    11461 ttggataaat caacagtaaa tacgatcgta tacggaaaat aatgcgatag tgaaactata
    11521 aaatctggca aaagattgct ttttgccaga ttttttttat aaaaagagga tcaatgtttg
    11581 ttagttcgaa agaaaggtgt ataatttaat tgaaaaatac ataaacagaa agtagaggac
    11641 aaagaatgat tatcccaaat agtttagaag aattagcagg ctacgtagaa actggaaaga
    11701 gtgtattttt ctttactgcc ggctggtgcg gagactgccg ttttatcaaa ccgcaaatgc
    11761 ctgaaattga aaatgacttt tctgattggc aatttattga agtagaccgt gacaagtaca
    11821 tagatgtagc cgctgaatgg aacatttttg gtatcccaag ttttgtcgtg atccaagacg
    11881 gaaaagaatt aggtcgtcta gtgaataaag atcgtaaaac aaaacaggaa atcgaatcgt
    11941 tcctagaaag tgtaacaaaa ggctgacatc tgttaggagg ggtcaagatg aatcaagaat
    12001 ataagaatat tttagtaggt atcgacggta gtgaacaagc acttgctgct ttcaaaaaag
    12061 cggtagaagt ggctcgccgt aataatggga aagtatatgt agcaaacgta atcgatcagc
    12121 aattttataa ctttatgggc tatgcaccta ttgatcaaaa tctagtcgat cagcaaactg
    12181 aaacagccaa ggaaatgatc gaagaatgta aagaatatgg gaaatctgtt gattacacaa
    12241 atatagaagg aattattgca tatggttctg ttcgtgaatc catggcagtc aaattgccaa
    12301 aaaaatatga gatcgatcta atcatggttg ggcagtcagg tctaaatgca gtcgaacgct
    12361 ttatgacagg aagtgtagcc agctacgtga tacgtcattc accttgtgat gtcttgatcg
    12421 tttctgacga agaagaacag ccaagtaatt aagcaagtgg aacttgtacg acctttttaa
    12481 tcaattaact aaaacagtaa agggactgtg acaaaagtct tgtcacagtc ccttgttacg
    12541 aataaatggt gttccagaag aggcttcttc ggaaataaag catgaaaaaa atttcatgca
    12601 gtgaatgcaa ttttttgtta agataaatct cagcaaagga taatggagga aagaacatga
    12661 tttttgctta taacaaagca cacgtcggag acaccttgat ggttattata gccgacgata
    12721 aaggaacaga aaacactgtt gaacgcaaat ggaatgttgc tcggatcaaa gatgaacaag
    12781 gtcaagttgt tgcttggaac tttttccata tctccgatca tctaacaatc gaagggaacg
    12841 gccaagtaag cttaacagat gaggaattag cgaaattgaa tcgattgatc aaagaagcag
    12901 gattcgaaga acagcttgaa gctgatcatg aaccgaagat cgttgtcggg tacgtaaaaa
    12961 catgcgtccc acatcctgat tctgaccatt tatcgattac tgagacagaa gtagacaatg
    13021 gtacagtatt gcaaatcgtt tgcggtgcac ccaatatcga agctggacaa aaagttgtcg
    13081 ttgcaaaacc aggagctatg atgccagatg gaatgatgat ctggccagga actttgcgag
    13141 gggtagatag ctatggaatg atctgttccg ctcgtgaatt gcatttgcca aatgctccag
    13201 caaaaaaagg aatcttagta ttgcctgagg atgccgtagt cggagaagca tttccaaaat
    13261 aaagaggttg tgacaagtct ttgtcacaac ctctttttta ttaattttca ctattttgat
    13321 tttgttttaa tgccgattga tcgatcgtca attcaacagt agttgttttt tctttcgagc
    13381 cttcatagta agtgattttg acagaatctc ccactttttt cttatacaaa gcagattgaa
    13441 gttctacacc agaagaaact tctttgtcat cgatcttagt aatgacgtcg tattgtttca
    13501 atccagcttt ttcagccggt gtagctgttt ggacatttgt cacgatgaca ccgttagtta
    13561 cagatgaagg aatcttcaag atttgttctt gttgctgagt agaaacggca gataagtcga
    13621 ccattgtgat acctagagct ggacgtgtca cttttccgtc tttttccagt tgattgatga
    13681 tattcactac atcgttactt ggaatagcaa atcccattcc ttctacgctt acattagaag
    13741 tagattcaga agtacttgcg atcttacttg agttgatacc gataacttgt ccttcgatat
    13801 taactaaagg accaccagaa tttccagggt tgattgctgc atctgtttgg atcgcattga
    13861 tattgactgt ttcgttcgat tcattcgtac ttgtcacttg tcggttcaag gaagaaatga
    13921 ttcctgaagt gacagaattt gcatattcag atcctagtgg ggaaccaatc gcaatcgcag
    13981 gctcaccaac cttcagagca cttgagtcac caaaagaggc aactgtttcg actttgtctg
    14041 aattgatctt caacaccgct aaatctgaat aagcatctgt tccaactaat tcagctttta
    14101 ccttcgtacc gtctttcatc aagacttcaa gaccttgctg gccatctact acgtgattat
    14161 tcgtgactac ataagcagta ttcccgtctt ttttgtagat cacgccgctt ccttcactat
    14221 aagcttccaa attactatca tctgagctgc tactttcttc ttgctgcccg aatagtccgc
    14281 caaaaccgct tgattggttt tgactttgta agttgataac agaaacgaca gcgtcttgaa
    14341 ctttatctac cgctttggtg atatcagaat ctacattgac tttgacattt tccacaactg
    14401 tttcaccggc agaattctga ttgccgcttg tagcagtaga actattgttg ccagtgccca
    14461 tcgctgcata aaaaatgcca gctgtcagta gtcctccaac gatccctcca acaagcccca
    14521 gacctaactt acgccagagg ctattatttt tacttttttt catctttggt gtcacatttt
    14581 ttctatccat gtgttcctcc tccaattaca ttcatttatc tactgttagt atagtcttat
    14641 ttgttgactt tagtctactt tgagactgtg aactttttat gaaaaacttt aggaaaaatc
    14701 attgttttca gatgaaaata tttgattttc tttttccaca gatactgtgt aaaactacgc
    14761 ggataggatt ggaaaaataa gggataaaac atatccacag cctgtgtata aagtggataa
    14821 attcttataa aacgtatgtt tgcctaatga atgtgatgaa ataaggtaaa ataacactgt
    14881 tttctgtgga taaacctgtg gatgttgtgc ataactgttg gggaaatctt tttaacaaaa
    14941 atagccaaaa taaacatata gacgaaaaaa ttgattataa taaacaaagt gagcatgaag
    15001 accaaaggag agaaaacatg aatatcaaaa ttatctcagt aggaaaatta aaagaaaaat
    15061 acctgatcca aggtatcaat gagtatgtca aacggctgaa tgcctatgca aaaatcgaat
    15121 tgatcgaagt tcctgacgaa aaagccccgg agaatttaag cgaagcgcaa atgagacaag
    15181 taaaagaaaa agaaggggaa cggatcttag cgaaaatcaa agaacaagaa tacgtctatg
    15241 ccttagcaat tgaagggaaa aatcctacaa gtgaatcttt tgctaaacaa atcgaccaat
    15301 taggtatcca aggaaaaagc caccttgttt ttgttatcgg cggctcttta ggactcagcg
    15361 aagccgtcat gaaaagaagc aatgctcaga tctcttttgg aaaaatgacg tatccacatc
    15421 aattgatgcg tctgatcctt gtcgagcaaa tctaccgtgc cttccggatc aatgcggggg
    15481 ctccatacca caaataatga tattttttaa gagaaaaaat tatccctatt ttaaataata
    15541 gcaataggta gtggtaggga gaaatttata aataactttt ttatttgctt tggtagccaa
    15601 gtaagttgca aaagaaaaat agatactgtt aaaaagataa tgtagacata gaaacaaata
    15661 cgtaaaattt tagttagaat aatggcaagg agaaaaaaca tgatagatga aaaaaagtgt
    15721 ttaaaaaatc tattagagga tatggatgat attgaagaaa actatctaaa tggactggga
    15781 aaacaaatat atttcagata cttaagctat ctaaaaatga aataagacat agtaattttt
    15841 tagctttttt gtttgctcca aatgaaaccc acaatttatc tgatgagttt tttaaaatg
//
LOCUS       DARBET010000060        15188 bp    DNA     linear   BCT 07-NOV-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4027
            SAMEA114569841-rid20112183.denovo.062, whole genome shotgun
            sequence.
ACCESSION   DARBET010000060 DARBET010000000
VERSION     DARBET010000060.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA114569841
            Sequence Read Archive: ERR12203666
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 15188)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15188)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001972618.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001972618.1
            Genome Coverage       :: 132x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/07/2023 13:18:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,041
            CDSs (total)                      :: 2,975
            Genes (coding)                    :: 2,908
            CDSs (with protein)               :: 2,908
            Genes (RNA)                       :: 66
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 67
            CDSs (without protein)            :: 67
            Pseudo Genes (ambiguous residues) :: 0 of 67
            Pseudo Genes (frameshifted)       :: 26 of 67
            Pseudo Genes (incomplete)         :: 28 of 67
            Pseudo Genes (internal stop)      :: 20 of 67
            Pseudo Genes (multiple problems)  :: 6 of 67
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15188
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA114569841-rid20112183.denovo.062"
                     /isolate="VRE4027"
                     /db_xref="taxon:1352"
                     /country="Denmark"
     gene            278..787
                     /locus_tag="SCJ86_002087"
     CDS             278..787
                     /locus_tag="SCJ86_002087"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305710.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HEG1169617.1"
                     /translation="MVQGIERGVYVVDKIQFNQVIEEFIKVNNLMTHIQKRPIRITND
                     LKLSTSMIHLIDVIGNYPETSITELADRLGVTKGAVSQQIPTLEKMNLISISQKKGNK
                     KTKLVSLTKYGRKVYESHNYLHEELYSSINEALESFSPDQISTIKQILEKVSYTINEY
                     QVSLTRKED"
     gene            880..2042
                     /locus_tag="SCJ86_002088"
     CDS             join(880..1156,1156..2042)
                     /locus_tag="SCJ86_002088"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611768.1"
                     /ribosomal_slippage
                     /note="programmed frameshift; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
                     /protein_id="HEG1169618.1"
                     /translation="MSRRQRRTYSKEFKQQVVDLYLAGKPRAEIIRDYELTTSAFDKW
                     VKQAQTTGSFQEKDNLIPEQVELIALRKKNKQLEMENDILKQAALILGPKRQVIDTNK
                     HKYSISAMCKVLNISCQTYYYQAKPVEDESELEELVQEEFIRNRKAYGTRKLKQCLAN
                     LGLQVSQRRIGRMMKQRGLRSTYTLAHFKFHSSTCNEAKTANILDGTFLQEKSLEAIV
                     TDLTYVRVGKKWQYICLILDLFNREIIGYSCGEKKDATLVKEAFAKIPYSLNNVQLFH
                     TDRGKEFDNQTIDEILWAFNITRSLSKKGCPYDNAVAESTYKSVKVEFVHQYRFETLA
                     NLRLELFDYVHWWNTIRLHGTLGYETPISFRQQRLAQRILDNELGCDTGGEAP"
     gene            2322..3302
                     /locus_tag="SCJ86_002089"
     CDS             2322..3302
                     /locus_tag="SCJ86_002089"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016179831.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HEG1169619.1"
                     /translation="MKKIGIREVAKEANVSPTTVSRVLNDRGYLSEETKRKVFDAMKK
                     LEYYPNELARALFKNETYTVGLIFPTITNPFHAELIQDIEFELSSRGYKVLLCNSLNN
                     PTKEKEYLTMLRKNQVDGIIVGTHNKDIADYDIPGLPIVAIDRSLGENTVTVSCDNYR
                     GGELATQLLVNHGCQNILCIRGDSKIKLPANSRTLAYKDVLNKFMLEPLVLEVPFVKE
                     INEKNEIIKTYLETHPNIDGVFAGDDLLASLAINNLGSSGKRVPKEVKVIGFDGAQQT
                     LIYNPELSTIQQPIDQISKVAVKKLLNMIKGEKETDELSLPVKLIKKNTA"
     gene            3424..4842
                     /locus_tag="SCJ86_002090"
     CDS             3424..4842
                     /locus_tag="SCJ86_002090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="family 43 glycosylhydrolase"
                     /protein_id="HEG1169620.1"
                     /translation="MEKLLIKSNNHQLYYQPKDVWVGDVMPYGKEGKFFIYHQRDTRN
                     PVPFGEPFGWALATTKDFVHFEDFGESLLRGDDNERDQFIYAGSVFEANGKVHAFYTG
                     YNREFLAQGKTSQVLLHAESDDFVHWEKSETALELEPQEGYDIRNWRDPFVVWDDEKE
                     EYLLILGARKGQDKHLQTGRLVEFTSKDLTNWEFKGDFWAPGLYTMFEMPELFKIGDW
                     WYLVYSEYSDGNKILYRMSQSLEGPWIKPKDEAFDGRAYYAGRTAYDGKRRVLFGWVP
                     TKDQENDKNNYLWGGTLVPHEVYQKEDFSLGVKPIDEVWNAFINLHDIADVEIETADT
                     REEVTLVKNSGTLFNLETTISFDENLSDFSLRLYKNLETDESYEYRFNLDEGRLTFDK
                     NPCYPWFQYMDKGLIRPISLKPNQSYKLKVIVDDNIMTLYIDGIALNARVHGCFGQAI
                     TITATNGSMTAINTKFSNEIKK"
     gene            4913..6382
                     /locus_tag="SCJ86_002091"
     CDS             4913..6382
                     /locus_tag="SCJ86_002091"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016179833.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HEG1169621.1"
                     /translation="MDYKKIAEDVLENVGGKENISSATHCITRLRLILKDKSKANMEM
                     LEEIEGAKGVLFNSGQLQVIFGTGIIEKVYKEFVELTGAKEVSVSEMKDEGVNNSGNR
                     LQRAFKVFSDIFIPIIPAFIGAAMVLGIRSLITTPGMFGMDGSFADHNVFLNDLSKFL
                     GIIATTFDYLPVLVMYSATKRFGGNPILGLLVGFVMIHPGLANRNDFVMGTIKPDYWH
                     LFGLSIPAVAFQGGVFPAILTSWFLAKVEKITNKYSPQVLAYILVPTVTIVLANFALF
                     LVFGPLGNFIGNVLGGIIDFLYMKMGAFGAFVFAGALQPLVVTGTQHAIQGIEANLVA
                     TTGFNYIQPIWSVSIIAQGGGCIGMYLLFKKKSKDRDIAISSFIPTLVGITEPAIFAV
                     NLKYSIVPFVCSFIGAGFGGAFMKIFDVKAIGQGLTVLPGLTIANPVLPYIFGNLIAF
                     ILPIIFILAYDKYKVFIPEAKHRKFAEKTDSKKLPVQVK"
     gene            6392..6859
                     /locus_tag="SCJ86_002092"
     CDS             6392..6859
                     /locus_tag="SCJ86_002092"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289220.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucose transporter subunit IIA"
                     /protein_id="HEG1169622.1"
                     /translation="MLNFFRKNEISVVAPATGVFEKLEKLSDPVFSKGMMGQGFAIDA
                     KNETIVSPIDGFVSSIFPTKHALGIKTKSGIEILVHVGIDTVELNGEGFDIKVQEGQK
                     VKAGDILMTINFVVFEKNHKTKDVIVVFPDYQDEIFIPKLEEVKQGQELFVLG"
     gene            6895..7767
                     /locus_tag="SCJ86_002093"
     CDS             6895..7767
                     /locus_tag="SCJ86_002093"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289219.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family protein"
                     /protein_id="HEG1169623.1"
                     /translation="MVFGAIEAGGTKFVCAIGDKDLNIFEKVSFPTTTPKETMNLVLD
                     FFNHHKDDLEGIGVGSFGPIDIHCESKTYGFITSTPKLAWQDFDFIGSLKKQLNIPTA
                     WTTDVNASCYGEYVSGNGRGLSSVVYYTVGTGIGGGAIQNGNFIEGFSHPEMGHMLVK
                     RHNKDNFSGTCPFHGDCLEGLAAGPAIERRSGKKGQEVTENDVTWEIEAYYIAQCAYN
                     TTLMLSPDVIIFGGGVMKQKHLKKKVQDKFIELINGYVAIPDVDNYIMTPKLLDNSGI
                     VGCLALARDVKEKS"
     gene            7848..10382
                     /locus_tag="SCJ86_002094"
     CDS             7848..10382
                     /locus_tag="SCJ86_002094"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353084.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma 54-interacting transcriptional regulator"
                     /protein_id="HEG1169624.1"
                     /translation="MKKKMKDEVLEYIKNNLKYYDFSAQDIAMKFCIKRNVASHYLNQ
                     LFSDGKLLKNDSVRPVMFKYNQQKPKDCFSKFIGADISLKSTIDKCKATVMYPPNGLP
                     LIIKGNSGVGKSFLASLIYQYALDRKVIHNDAKFVVVNCADYANNPELLSAVLFGYKK
                     GAFTGAEKDTAGMLSSANGGYLFLDEVHNLSAENQEKLFLLMDSQKYRKLGESVQWEY
                     ANVRLILATTEDKNTSLLATFRRRIPAEITLPDYNNRSTSEKIQLLFEFLKDEAITID
                     SKIFCSIELFTDLLSKTFEGNVGELRNEIKLLCAEGYLGNKRKSSIYLGDKLDSGFWI
                     DPEISIDLKKMFLKSLSEIDLIELFQNHISIEESMSQLRDRILKECAGSSEYNYLESD
                     EFIALRNYVVNKISPIIDSTGLCLLDEFLADISLFIMFIDVIEETSRNFLLSKFRKFR
                     MKNDKQKLLANEIWSSLEVEESKQELLLKWILFLVKKFYVKIPETHCLIVMHGKITAS
                     AIARETNKLLNTYVYEAFDMPIEGETSDLINLINNFCKSIDTTNGLILLVDMGSLEQM
                     YEKIESNVIGDLVILNNVSTALAIECGIQVCQKKPISHFYQMDFDSFQVKVQYYKGLS
                     QKKNVIVSCLSGEGISEKVKDILKRYLDNQVEILIFDFNALKKIAKEKDTMIFKNTIC
                     VLSTTEFYIQGIDCLNLENLINGNQTLEKLNKYMDSDSCEFCLNELVKLFTIEGASTK
                     LRFLDSKKVFNEIEKVLYLYEKYYDVKIPSFLRINLFLHLSGMIERIMIGDGITNEHL
                     KEGAQLFDTFLNVSKDFFSEIKDIYCIEIPKGEYELIYTIFEQTIF"
     gene            10538..11311
                     /locus_tag="SCJ86_002095"
     CDS             10538..11311
                     /locus_tag="SCJ86_002095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014387092.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HEG1169625.1"
                     /translation="MAFWQTFLVVLYGFFINWDKEGPKIGTSQPVVAGFLTGLIMGDI
                     QTGLYIGGTLQLMTLGITSFGGASVPDYQTAALIGTYLAISTNQDASIGITLAIPVAM
                     LIVQLDVLKWSTNIFFQQKAEKFAQKEDYQKLEWMQYLASINTSFVSGIPVLLTVILG
                     PTLVGSAINYIPEWVTGGLKVAGGLLPAVGIGMLLKYLPTKDYFAYLLVGFVLAVYFQ
                     IPILGISLLGAAIALIVYKNRSNNTQVAPAATGGFDEDE"
     gene            11304..12095
                     /locus_tag="SCJ86_002096"
     CDS             11304..12095
                     /locus_tag="SCJ86_002096"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289216.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS system mannose/fructose/sorbose family
                     transporter subunit IID"
                     /protein_id="HEG1169626.1"
                     /translation="MNKKLTKIDKQVIFRWILNGATSLNYEKMEGLGYAYSMLPFIKD
                     TYKDDPEGMKQSVLAHLQFFNTTPYTAPYIIGMNVGIEKEEKNKSLEAVSALKTGLMG
                     PLAGIGDSVFVVIPWTIFGAIAANMALEGSFVGIILWILASILLRITAYPLYLAGKES
                     GASLLTQIETKLKSITDAVSILGLMVVGALIATVVKVNVGFEFKQGDLTMTGNEIFDQ
                     VMPGLLPACVVAIVYYLLGRKVKPVYVILLILVVSIALRALNILK"
     gene            12105..12596
                     /locus_tag="SCJ86_002097"
     CDS             12105..12596
                     /locus_tag="SCJ86_002097"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289215.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HEG1169627.1"
                     /translation="MDEKMKGIIHVRIDDRMIHGQVATQWTNQLSANRIMVINNEVAN
                     DDVKKAVVRLAAPPNVRTSIITREVAVKNILSGKYEGQKVLIVAVSPIDVKFLIDNGL
                     PITSVNVGNLSRRNGTERIRPTINITEEEREAFKELISDGVEVTVIQTPRDSKEFLKD
                     FIY"
     gene            12614..13024
                     /locus_tag="SCJ86_002098"
     CDS             12614..13024
                     /locus_tag="SCJ86_002098"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289214.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HEG1169628.1"
                     /translation="MEFIITSHGNIAIGFVDTLKMFFGDNLNCHVVTLGDAGIEEFRE
                     NIEKIVSQVKNQEVMVFADLIGGTPFNEFAKRSEVFQNGFQLLGGMNLGILIEAVNLR
                     LQGKKSDDIIENLRNMNTIACLADMQRHTNEEDE"
     gene            13028..14428
                     /locus_tag="SCJ86_002099"
     CDS             13028..14428
                     /locus_tag="SCJ86_002099"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296814.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-fructofuranosidase"
                     /protein_id="HEG1169629.1"
                     /translation="MQKIFYKPKDFWFGDCMPFYKDGTFYLFHQRDTRNPKPLTDPFG
                     WSLVTTTDFVKFEEYGEVIHRGGNDDIDQYIYAGSIVQGINEYLALYTGHNRKAKLDG
                     KTSEVLMIASSKDLIHWKKDGEAAKLVPQEGYDKNDWRDPFAIFDQDHGKYVLILGAR
                     KPASKKKPTGRLVYFESEDLKDWNFKGDYWANNEFNMIEMPDIFEIDGTWYLLFSEYC
                     EDKKTKYRVGAGLFSDWKSPVDEAFDGKAYYAARTVGTNETGRFLTGWVATKEGENDQ
                     NNWDWGGALVPHQIIQKTDKTLGVKLPEKIIENFKEITPFSSFKLEKEFGKKERVIVP
                     KIEDYFLFETTLTITSLTKEFALKLFVDSDTQEGYEFRISLINNQLTFGKTPNYPWPQ
                     MFDKGLQRPLFLEIGKEYELKLIVDDTIAVLYIDNVALSTRMYDKVGTALSMTVTEGA
                     IKVDKLDYSINYECGF"
     gene            14451..14825
                     /locus_tag="SCJ86_002100"
     CDS             14451..14825
                     /locus_tag="SCJ86_002100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016179839.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HEG1169630.1"
                     /translation="MKLNGLQHIGIPTKDFGASKIFYESLGFSLINEEMNVGSKVGFF
                     ELNGTIIEIWEDKTTECRGAIDHLALDTDDIDEVFNSIQDLGYELLDKNIMELPFWEN
                     GIRYFNFYGPNHEIIEISQMKK"
BASE COUNT         5272 a         2129 c         3041 g         4746 t
ORIGIN      
        1 ttttttattt gtctcgctta attatacaaa cggcgtgata aaaaacgaga aatgaattat
       61 aggtttagac ctttatcatt tctcgtttta ttttattgta tcaaggctct atcataagtg
      121 gtaaaatcta cttttaaatc acatgtaatc attgataagt ccagtgtttt agcggataat
      181 cagcttttta tttatttttt cttataaatt tgataaatag aaattagtat agccctaaac
      241 aaattatttg tgttttattg tttagggcta tactatattg gtacaaggaa tagaaagagg
      301 tgtttatgtg gttgataaaa ttcagtttaa tcaagttata gaagaattca tcaaagtaaa
      361 taatttaatg acccatattc aaaaacgacc aatcagaata actaatgatc taaaactcag
      421 tacgagtatg attcatttga ttgatgtgat tggaaattat cctgaaacta gtattacgga
      481 attagctgat cgattaggcg ttaccaaagg agctgtatcc caacagattc ccactttaga
      541 gaaaatgaat cttataagca tttctcaaaa aaaaggaaat aagaaaacta aacttgtatc
      601 gttaacaaaa tatggtcgaa aggtgtacga atcacataat tatttacatg aagagcttta
      661 tagctcaata aatgaagcgt tagaatcatt ctcaccagac caaatttcaa ccattaaaca
      721 gattctagaa aaagtatctt atacaatcaa tgaatatcaa gtaagtctga caagaaagga
      781 agattgacat gaaaaaaatg atttttgaat cggctacact agactagaca gaaaaaataa
      841 ggtgtgtaaa ataaatgaaa acacactgga ggaattttta tgtcaagacg tcaacgtaga
      901 acctattcaa aagaattcaa acaacaagtc gtcgatcttt atctcgcagg aaagccccga
      961 gctgaaatca ttcgagatta tgagctgact acctcggcct ttgataagtg ggtcaagcaa
     1021 gcgcaaacta ctggctcgtt tcaggaaaaa gataacttga tccctgaaca agtagaacta
     1081 attgcattac gaaagaaaaa taaacagctt gaaatggaga atgacatttt aaagcaagcg
     1141 gcgctgatat tgggacgaaa agacaagtaa ttgataccaa taagcacaaa tactcaatat
     1201 cagcgatgtg taaagtgtta aacatctctt gccaaaccta ttattatcaa gccaaaccag
     1261 ttgaagatga atcagaactg gaagaactcg ttcaagaaga atttattcgc aatcgaaaag
     1321 cttatggtac caggaagctg aagcagtgct tggccaatct aggtcttcaa gtgagtcaac
     1381 gcagaatcgg ccgtatgatg aaacagcgtg gcttgcgttc aacatacaca ttggctcatt
     1441 tcaaattcca ttcttctact tgtaacgaag ccaagaccgc caatattcta gacggaacct
     1501 tcctacagga aaagtcatta gaagcgattg taaccgacct tacctatgtt cgtgttggga
     1561 agaaatggca gtatatctgc ctgattcttg atttgtttaa tcgagaaatc atcggctatt
     1621 catgcgggga gaagaaagat gcgacgttag tcaaggaagc ttttgcaaag attccgtact
     1681 cgttgaataa tgtacaactt tttcacacgg atcgtggaaa agaattcgat aaccagacga
     1741 ttgacgaaat tttatgggcc tttaatatca cgcgttcctt aagcaaaaaa ggctgcccct
     1801 atgataacgc cgttgcagaa tccacctata aatctgtcaa agtagaattc gttcatcaat
     1861 accgctttga gacattggct aatttgcgat tagagctatt tgattacgtc cattggtgga
     1921 ataccatccg gctgcacggc acgttaggct atgagacacc gatcagtttt cgacagcaga
     1981 gattggcgca gcgaatcctt gataatgagc tcggatgtga taccggtggc gaggcacctt
     2041 aattatttga atgagcttct gccttagaga atcagattcg aggaggctcg ttgtcaaggg
     2101 cagtcggaac accagcagca agcttacgca ataccttata atttttgtca aaaaaagtgt
     2161 tgccattcca catatatctt taaacagcta ttgccagata aaaataaatg atattatata
     2221 tataaatact tatccgaaaa ggggaatgag agagtcaagg ttcttggaat gtcgaatagt
     2281 cgagtaggta gattttctat ctacaacctt gttgtttaaa aatgaaaaaa attggaatta
     2341 gagaagttgc gaaagaggca aatgtttctc ctacgactgt gtcgagggta ttgaatgatc
     2401 gtggatattt gagtgaggaa acaaaaagaa aagtttttga tgccatgaaa aaattagaat
     2461 actatcctaa tgaactggcg agagcacttt ttaaaaacga aacatatacc gtaggtttga
     2521 tttttcctac aattactaat ccatttcacg ctgagttaat tcaagatatc gagttcgagc
     2581 tttccagcag gggatataaa gtgttgctgt gcaatagctt aaacaatcca acaaaagaaa
     2641 aagagtactt gacgatgttg agaaagaatc aggttgatgg aattatagta ggaacgcaca
     2701 ataaagatat tgcggattat gatattccag ggctacctat tgtagcgatt gatagaagtc
     2761 ttggtgaaaa tacagttaca gtatcttgtg ataattacag gggtggggaa ttagctactc
     2821 aactacttgt aaatcatggc tgtcagaata tactgtgtat tcggggagat agtaagataa
     2881 agctacccgc gaatagtagg actttagcat ataaagatgt tttgaataaa ttcatgctgg
     2941 aaccgttagt cctagaagta ccatttgtaa aagaaatcaa tgaaaaaaat gaaataatta
     3001 agacatatct ggaaactcat ccaaatattg acggggtttt tgcaggagat gatttattgg
     3061 cttctttggc gattaacaac ttaggaagtt cgggcaaaag agttccaaaa gaggttaaag
     3121 tgattggatt tgatggagct caacaaacat tgatttataa tccagaatta tcaactattc
     3181 aacagcctat agatcaaata tctaaagtag cagtaaaaaa attattgaat atgattaagg
     3241 gcgaaaagga gacggatgaa ctttcacttc cagtaaagct cataaaaaaa aatacagcat
     3301 aagttaaatc ggcgaaaaat tccttcgtcg atttaaaaat atttttatga aacgggttga
     3361 catatgaaac gggttgacat atattagttg tgaagataaa aaccttttca ctggaggaag
     3421 aaaatggaga aattattaat taaaagcaat aatcatcaac tttactacca acctaaggat
     3481 gtttgggtcg gagatgttat gccttacggg aaagaaggaa aattctttat ttatcatcaa
     3541 cgtgatacta gaaatccagt tccttttgga gaaccgtttg gctgggcttt agcaactacg
     3601 aaagattttg ttcattttga agactttggg gaatctcttt taagagggga tgacaatgag
     3661 agagaccagt ttatctacgc tggttctgta tttgaggcaa atggaaaggt tcacgcattt
     3721 tatacagggt ataatcgtga attcttagcg cagggaaaga catctcaagt gttgcttcat
     3781 gctgagagtg atgattttgt tcattgggaa aaatcagaaa cagcattgga gttagaacca
     3841 caagaagggt atgatattcg aaactggaga gatccatttg tcgtttggga tgatgaaaaa
     3901 gaagaatatt tgctaatttt aggggcgcga aagggtcagg ataaacatct acaaaccgga
     3961 cgtttagtgg aatttacatc aaaagattta actaattggg aattcaaagg tgatttttgg
     4021 gctccgggat tgtataccat gtttgaaatg ccagaacttt tcaagattgg tgactggtgg
     4081 tatcttgtct attcagaata ctcagatgga aataaaatac tctatcgaat gagtcaaagt
     4141 ttggaaggtc cttggattaa gcctaaagat gaagcttttg atggacgggc ttattatgca
     4201 ggtagaacag cttatgatgg aaaacgtcga gtcctatttg gatgggtacc aacaaaagat
     4261 caagaaaatg ataaaaacaa ttatttatgg ggaggtactt tggttcctca tgaagtctat
     4321 caaaaagagg atttttcact tggggtaaaa cccatagacg aagtgtggaa tgcattcata
     4381 aatttacatg atattgccga tgtagagata gaaacagcag acacaagaga agaagtaacg
     4441 cttgtcaaaa atagtggaac tttgtttaat ttagaaacaa caatttcatt tgatgagaat
     4501 cttagtgact tttcactgag attatataaa aaccttgaga cagatgaatc ttacgaatat
     4561 cgcttcaatt tagatgaggg gcgtcttaca tttgataaaa acccttgtta cccttggttt
     4621 caatatatgg ataaaggact tattagacct atatctttaa agcctaacca atcttataaa
     4681 ctaaaagtga ttgttgatga caatataatg acactttata ttgatggaat cgctcttaac
     4741 gctcgtgtac atggttgttt tggtcaagca attactatta cagcaacaaa tggatcaatg
     4801 actgcaataa ataccaagtt ttcaaatgaa ataaagaaat aataaacgtc aatcatgtgg
     4861 caggtctgaa aatcaaacat gacctgtcac atttaaaagg gaggataaat gaatggacta
     4921 caaaaaaatt gccgaagatg tattggaaaa cgttggagga aaagagaata tctcaagcgc
     4981 tacacactgc attactcgtt tacgtttaat tcttaaagac aaaagcaaag cgaatatgga
     5041 aatgcttgag gaaatagaag gtgcaaaagg tgttcttttc aatagtggac agttacaagt
     5101 aattttcgga acaggtataa ttgaaaaggt ttacaaagag ttcgttgaat tgacaggagc
     5161 taaggaagta tccgtttctg aaatgaaaga cgaaggagtc aataattcag gaaatagact
     5221 tcaacgagct tttaaagttt tctcggatat ctttattccg atcattccag cttttattgg
     5281 ggcagcaatg gttttaggaa ttcgttcgtt gattaccact cctggaatgt ttggaatgga
     5341 tggctcattc gctgatcaca atgtattcct aaatgattta tccaaatttc tgggtattat
     5401 tgcaaccaca tttgattatt taccagtttt agttatgtat tctgcaacaa aacgttttgg
     5461 cggaaaccct attttaggat tacttgtagg atttgttatg attcatccag gactagccaa
     5521 tcggaatgat ttcgtcatgg gaactataaa gcctgattat tggcatttat ttggattaag
     5581 cattccagca gttgcgtttc aagggggtgt ttttccagca attttgactt cttggttttt
     5641 agcaaaagtt gagaaaatta caaataaata ttctcctcaa gtacttgctt acattttagt
     5701 cccaactgtg acaattgttc ttgccaactt tgcactattt ttagtgtttg gtccattggg
     5761 gaattttatc ggtaatgttt taggtggaat cattgatttc ctatatatga agatgggtgc
     5821 ctttggtgct tttgtttttg caggcgcgtt acaaccatta gttgtaacag gaacacaaca
     5881 tgctattcaa ggtattgagg caaacttagt cgcaacaact ggattcaatt atatccaacc
     5941 tatttggtca gtttcaatta ttgcccaagg tggcggttgt attggtatgt atttactctt
     6001 taagaaaaaa tctaaagata gagatattgc gatttcaagc tttattccaa cactggtagg
     6061 tattactgaa ccggctatct ttgctgttaa tttgaaatat tcaatcgttc cttttgtttg
     6121 ctcgtttatt ggtgctggtt ttggtggagc atttatgaaa atttttgatg taaaagccat
     6181 tggacaagga ttgactgtat tgccaggtct gactattgct aatccagttc ttccttatat
     6241 atttggtaat ctcatagcat tcattttacc aatcattttt attctcgctt atgataaata
     6301 taaagtgttt atccctgaag ctaaacaccg taaatttgca gaaaaaactg actcaaaaaa
     6361 attgccagtt caagtaaagt gagatgagag catgttaaat ttttttagaa aaaatgaaat
     6421 aagcgttgta gcacctgcaa ccggtgtttt tgaaaaactg gagaaattaa gtgatccagt
     6481 cttttcaaaa ggaatgatgg gacaaggatt tgcaattgat gcaaaaaacg aaacgattgt
     6541 atctccaatc gatggttttg tttcaagtat ttttcctaca aaacatgcct taggaataaa
     6601 aacaaaatca ggaattgaga tacttgttca tgtaggaata gatacagttg agctaaatgg
     6661 tgagggattt gatattaaag ttcaagaagg acaaaaagtt aaggctgggg atattcttat
     6721 gaccattaat tttgtagttt ttgaaaagaa tcacaagact aaagatgtca ttgttgtttt
     6781 tccagactac caagacgaaa ttttcattcc gaaacttgaa gaagtaaaac aaggacaaga
     6841 attgtttgtt cttggctaat gataagtaat taataataga atcgaggaaa atgtatggtt
     6901 tttggggcta ttgaagctgg tggaacaaaa tttgtttgtg ccattggaga taaagatttg
     6961 aacatatttg aaaaagtcag ctttcccacg actacaccca aagaaacaat gaatttagtt
     7021 ttagattttt ttaatcatca taaagatgat ttagaaggta tcggagtggg atcgttcggt
     7081 cctattgata ttcattgtga atctaaaacg tatggcttca ttacatcaac gccaaaactt
     7141 gcttggcagg attttgattt tattggaagt ttaaaaaagc aacttaatat tccaactgct
     7201 tggacgactg atgtaaatgc ctcttgctat ggtgaatatg tttctggaaa tggcagaggc
     7261 ctttcaagtg ttgtttacta taccgtagga acgggcatag gaggtggcgc tatccaaaac
     7321 ggaaacttca ttgaaggttt tagtcatcca gaaatgggac atatgcttgt aaaacgacac
     7381 aataaagaca acttttctgg gacatgtcca ttccacggag actgtttaga gggcttagct
     7441 gctggtccgg caatcgaaag aagatcaggg aaaaagggac aagaagtgac tgaaaatgac
     7501 gttacttggg aaatagaagc ttattacatt gctcagtgtg catataatac aacattgatg
     7561 ttatcacctg atgtcattat atttggtggc ggagtaatga aacaaaaaca cttaaaaaag
     7621 aaagtacaag ataagtttat tgagttgatc aacggatatg tcgctattcc cgatgttgac
     7681 aattatatta tgacaccaaa attgttagat aattctggta ttgtaggttg tttagcttta
     7741 gcaagagacg ttaaagaaaa atcttaattt ggaaaaaaga tcgtctcgac tatttgtttg
     7801 gttgatgcga tctttttttc aattagtaat tgaagggacg cctaaaagtg aaaaaaaaaa
     7861 tgaaagatga agtattagag tatatcaaaa ataatttaaa gtattacgat ttttctgctc
     7921 aagatattgc aatgaaattt tgtattaaaa gaaatgtcgc ttcccactat ttgaatcagt
     7981 tattttcaga tggaaaatta ctaaagaatg attctgttag accagtaatg ttcaagtata
     8041 atcaacaaaa gccaaaagac tgcttttcta agtttattgg agcagacata tctttgaaat
     8101 ccactataga taaatgcaaa gctactgtaa tgtatccccc taatggcctc ccattaataa
     8161 taaaaggaaa cagtggcgtt ggaaaaagtt ttttagcaag tttgatctat cagtatgctc
     8221 ttgatagaaa agtaattcat aatgacgcaa aatttgttgt tgtgaattgt gcagattatg
     8281 caaataatcc agagctattg tcagcagttt tatttggata taaaaaagga gcctttactg
     8341 gggctgaaaa agatactgca gggatgctat cttctgctaa tggaggatac ctttttttag
     8401 atgaggttca caatctaagt gcagaaaacc aagagaaatt gtttcttctt atggattctc
     8461 aaaagtatcg taaacttggt gaaagtgtgc aatgggagta tgcaaatgtt cgccttatct
     8521 tagccacaac agaagataaa aatacctctt tgttagcaac ttttagaaga cgcatcccag
     8581 ctgaaatcac tctgccagat tacaataata gaagtacatc tgaaaaaatt caattattat
     8641 ttgaatttct taaagatgaa gcaattacga ttgattcaaa aatattttgt tcgattgaac
     8701 tttttactga tcttttatcg aaaacatttg aaggaaatgt tggagaattg aggaatgaaa
     8761 tcaagcttct ttgtgcagaa ggttatttag gaaataagag aaagtcttca atttatttag
     8821 gggacaaact agacagcggt ttttggatag atccagaaat tagtatagat ttaaaaaaaa
     8881 tgtttcttaa atcactttct gaaattgatt tgattgaact ttttcaaaat cacatttcaa
     8941 ttgaagaaag catgagtcaa ttaagagata gaatacttaa agagtgtgca ggttcttctg
     9001 agtataatta tttagaaagt gacgaattta tagcattaag aaactatgtt gttaataaaa
     9061 tatcaccaat tattgattcg acaggtctat gtttattaga tgaattcttg gcggatatca
     9121 gtctatttat aatgtttatt gatgtaattg aagaaacatc aagaaatttt ttgctgtcta
     9181 agttcagaaa atttcgtatg aaaaatgata aacaaaaatt attggcaaat gagatttgga
     9241 gtagtttaga agtcgaagaa agtaaacaag aacttttgtt gaaatggata ctttttcttg
     9301 taaagaagtt ctatgtgaaa ataccagaaa cccattgtct gatagttatg cacggtaaaa
     9361 taacagcttc tgcgatcgca agggaaacaa ataagttgct gaatacatat gtttatgagg
     9421 ctttcgatat gccaattgaa ggtgagacta gtgacttaat taacctaatc aacaattttt
     9481 gtaaaagtat agatacgacg aatggactca ttctgctagt tgatatggga tcattggaac
     9541 aaatgtatga aaaaattgaa tccaatgtaa taggtgattt ggtaatttta aacaatgttt
     9601 ctacagcttt agcgattgaa tgtgggattc aagtttgcca aaaaaagcca attagtcatt
     9661 tttaccagat ggattttgat tcgtttcaag ttaaagttca gtattataaa ggattatcac
     9721 aaaagaaaaa tgtaatagta tcttgtctct ctggtgaggg gatttctgaa aaagttaagg
     9781 atattttaaa acggtatctc gataatcaag tggagatttt gatttttgat tttaacgcat
     9841 taaagaaaat agcaaaggaa aaagatacta tgatttttaa gaatacaatt tgtgttcttt
     9901 caaccactga attttacatt caaggaatcg attgtttaaa tttagaaaat ctaattaatg
     9961 ggaatcaaac cttagaaaaa ttaaataagt atatggactc ggatagctgt gagttctgct
    10021 taaatgaatt agttaaactc tttactattg aaggcgctag tactaagctt cgctttttag
    10081 attccaaaaa agtattcaat gagatagaaa aagtactata tttgtatgaa aaatattacg
    10141 atgtcaaaat cccaagtttt ctgagaatta atctatttct acatctttct ggaatgattg
    10201 aacgaattat gattggtgat ggaataacaa atgaacattt gaaagaggga gcgcaactat
    10261 ttgatacttt tttaaatgta tcaaaagatt ttttttcaga aatcaaagat atttactgta
    10321 ttgaaatacc caaaggagag tatgagttaa tctatacaat ttttgagcag actattttct
    10381 aaatagcata ctagcgtcta aaaaaatgaa agggtgtcca aagtatattt agacgctcat
    10441 ttaattaata tttactgtat tttcccgctt tattggataa tgttagttgg cacagtaatt
    10501 gcttatataa aagtataaga agaaaggaga gaacaaaatg gcgttttggc aaacattttt
    10561 agttgtgttg tatggatttt tcattaactg ggataaggaa gggcccaaaa tcggaacctc
    10621 ccaacctgtc gttgcaggtt tcttgaccgg acttattatg ggagatattc aaactggact
    10681 ttatatcggt gggactttgc aattaatgac actaggtatt actagttttg gtggtgcatc
    10741 agtaccggat tatcaaacag cagcattgat cggtacttat ttagcaatct caactaacca
    10801 ggatgcttct ataggaataa ctttagcaat accggtagca atgttaattg tacaactaga
    10861 tgttctaaag tggtcaacaa acattttttt tcaacaaaag gcagaaaaat tcgcacaaaa
    10921 agaagattat caaaaattag agtggatgca atatttggca tcaattaata caagctttgt
    10981 atctggaatt cctgttttgt taactgtgat attaggccca acgttagtgg gaagtgcaat
    11041 caattatatt cctgaatggg ttaccggtgg tttaaaggta gcagggggac tattacctgc
    11101 tgttgggata ggaatgctac ttaaatattt acctacaaaa gattactttg cctatttgtt
    11161 agttggtttc gttttggcag tttatttcca aataccaatt ttagggattt cgttacttgg
    11221 cgctgcaatc gcactaatcg tatataaaaa tagatcaaat aatactcaag tagcaccagc
    11281 tgcaacagga ggttttgacg aagatgaata agaaactgac aaaaatcgac aaacaagtaa
    11341 ttttccggtg gattctcaat ggtgcaactt cattaaatta tgaaaaaatg gaagggttgg
    11401 gttatgcgta ttctatgctt ccttttatca aggacacata taaagatgat cctgaaggaa
    11461 tgaaacaatc cgttttagct cacttacaat tttttaacac tacgccgtat acagcgcctt
    11521 acattattgg tatgaatgtt ggaattgaaa aagaagaaaa aaataagtct ttggaagcag
    11581 taagtgctct gaaaacaggt ctaatgggtc ctttagcggg aattggagat agtgtatttg
    11641 tagttattcc atggactatt tttggagcaa ttgcagctaa tatggcattg gaaggtagtt
    11701 ttgttggaat tattttgtgg atactggcat caattttatt aaggattact gcttatcctt
    11761 tataccttgc tgggaaagaa tcgggtgcga gcctactaac tcaaattgaa acaaaattaa
    11821 agtctattac tgatgcagta tcaattttag ggttaatggt ggtcggtgca ctcattgcca
    11881 cagttgtaaa agtgaatgtt ggctttgaat ttaaacaagg cgacttaaca atgaccggaa
    11941 atgaaatctt tgatcaagtt atgcctggtt tattacctgc atgtgtagta gcaattgtgt
    12001 attatttgtt aggacgaaag gttaaaccag tttatgtaat tctattaatc ttagtagttt
    12061 ctattgcgtt aagagcgtta aatattttga agtaggtgtt gaatatggat gaaaaaatga
    12121 aaggtattat acatgttcgt attgatgatc gaatgattca tggacaagta gcaacacaat
    12181 ggacaaatca attgagtgct aacagaatta tggttattaa taatgaagta gccaatgatg
    12241 acgtaaaaaa agctgtagtt cgcctcgctg caccacctaa tgttagaact tcaataatta
    12301 caagggaagt agcagttaaa aatattcttt ctggtaaata tgaaggacaa aaagttctta
    12361 ttgttgcagt ttctccaatc gatgttaaat ttttgattga taacggttta ccaataacaa
    12421 gtgtaaacgt aggaaattta tcaagaagga atggaacaga acgcatccgt ccgacgataa
    12481 atattactga agaagagaga gaggctttta aagaactaat tagtgatgga gttgaagtga
    12541 cagtgataca aactcctaga gattcaaaag aatttttaaa ggattttatt tattaagaac
    12601 agaggatgag tggatggagt ttataattac atctcatgga aatattgcca ttggatttgt
    12661 agatacatta aaaatgtttt ttggcgataa tttaaattgt catgtggtaa cacttggaga
    12721 tgcaggcatt gaagaattcc gtgaaaacat tgaaaaaata gtttctcaag taaaaaatca
    12781 agaagttatg gtatttgctg acttgattgg tggaacacct ttcaacgaat ttgctaaaag
    12841 aagtgaagta tttcaaaatg ggtttcaact tcttggtggc atgaatttag gaattttgat
    12901 tgaggctgtc aatttgagat tacaaggaaa aaaaagcgat gatatcatcg aaaatctaag
    12961 aaatatgaat acaattgcgt gtcttgcaga tatgcaaaga catactaacg aagaggatga
    13021 gtgataaatg caaaaaattt tttacaagcc taaagatttt tggtttgggg attgcatgcc
    13081 tttttataaa gatggaactt tttatctatt tcatcaacga gatacgagaa atcctaaacc
    13141 tttaactgat ccttttgggt ggtcactagt tacaaccact gattttgtga aatttgaaga
    13201 atacggggaa gtaattcatc gtggtggcaa cgatgacatt gatcaataca tttacgctgg
    13261 aagtattgtc caaggaatca atgagtattt agctttgtat actggacata atcgcaaagc
    13321 taagttggat ggaaaaactt cagaagtgtt aatgattgca agttctaaag atttaattca
    13381 ttggaaaaag gatggcgaag cagcaaaact tgttcctcaa gaaggatatg ataagaatga
    13441 ttggcgcgat ccttttgcaa tttttgatca agatcatgga aaatacgttt tgatactagg
    13501 agctagaaaa ccagcaagta agaaaaagcc gacggggaga ttggtctatt ttgaatccga
    13561 agatttaaaa gattggaatt tcaaaggaga ctactgggct aataatgaat ttaacatgat
    13621 tgaaatgcct gatatatttg aaattgatgg aacgtggtat ttactttttt cagagtattg
    13681 tgaagataaa aagactaaat atcgagtcgg agcaggatta ttttcagatt ggaaatcacc
    13741 agtagacgaa gctttcgacg gaaaagccta ttatgcagca agaacagtcg gaactaatga
    13801 aactggccgt tttctaactg gctgggtggc aacaaaagaa ggggagaacg atcaaaataa
    13861 ctgggattgg ggcggtgcac ttgttccaca tcaaattatt caaaagacag acaaaactct
    13921 tggtgttaaa ctgcctgaaa aaataattga gaattttaaa gaaatcactc cattttcaag
    13981 ttttaagtta gagaaagaat ttggaaaaaa agaaagagta attgttccta aaatcgaaga
    14041 ttattttcta tttgaaacaa ctttgactat tacatcgtta acaaaggagt ttgctttaaa
    14101 attatttgta gatagtgata ctcaagaagg atacgaattt cgtatttcat tgattaataa
    14161 tcagcttact ttcggaaaaa ctccgaacta tccttggcct caaatgtttg ataaggggct
    14221 gcaacgacca ctatttttgg aaataggcaa agaatatgaa ctaaagttaa ttgttgatga
    14281 tacgatagct gttttatata ttgataatgt tgctttaagt actagaatgt atgataaagt
    14341 cggaacagca ttaagtatga ctgttacgga gggagcaatc aaggtagata aactagacta
    14401 ttcaataaat tatgaatgtg gattttaaaa caattgaagg gatgatgtaa gtgaaattaa
    14461 atggtttaca acatattgga attccaacaa aagattttgg agcttcaaaa atcttttatg
    14521 aatccttagg gtttagtctg attaatgaag aaatgaatgt gggaagtaaa gtcggctttt
    14581 tcgagttgaa tggaacgata atagaaatat gggaagataa aactactgag tgtagaggag
    14641 ccattgatca tctagcatta gatacggatg acatagatga ggtattcaat tcaattcaag
    14701 atttaggata tgaattatta gataaaaata ttatggaatt accgttctgg gaaaatggaa
    14761 tccgttactt taatttctac ggaccaaatc atgagattat agagataagc caaatgaaga
    14821 aataaataga taacgaagtg ctctaagttg gtatgaacga aaagtacttc attttttggt
    14881 atcagcattt caatagttta atgagaattt actatttcaa attaattcaa agtgacagta
    14941 ttttttactt atttggtttc aaattttgta atccttccta tttattggaa tacactccac
    15001 attttgtata caagtcatat catgttttag caaagctctt accaagcaaa gttataatga
    15061 tgttttccaa agtttgatct ttcgatggag gctaacacat attttttgca ggacagccaa
    15121 aattagatgt atagaataga gtctgtgagg cgtatagaca gtttattgtc tgtatgcctt
    15181 tttttatt
//
LOCUS       DARBET010000061        15072 bp    DNA     linear   BCT 07-NOV-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4027
            SAMEA114569841-rid20112183.denovo.063, whole genome shotgun
            sequence.
ACCESSION   DARBET010000061 DARBET010000000
VERSION     DARBET010000061.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA114569841
            Sequence Read Archive: ERR12203666
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 15072)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15072)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001972618.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001972618.1
            Genome Coverage       :: 132x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/07/2023 13:18:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,041
            CDSs (total)                      :: 2,975
            Genes (coding)                    :: 2,908
            CDSs (with protein)               :: 2,908
            Genes (RNA)                       :: 66
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 67
            CDSs (without protein)            :: 67
            Pseudo Genes (ambiguous residues) :: 0 of 67
            Pseudo Genes (frameshifted)       :: 26 of 67
            Pseudo Genes (incomplete)         :: 28 of 67
            Pseudo Genes (internal stop)      :: 20 of 67
            Pseudo Genes (multiple problems)  :: 6 of 67
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15072
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA114569841-rid20112183.denovo.063"
                     /isolate="VRE4027"
                     /db_xref="taxon:1352"
                     /country="Denmark"
     gene            <1..170
                     /locus_tag="SCJ86_002101"
     CDS             <1..170
                     /locus_tag="SCJ86_002101"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HEG1169631.1"
                     /translation="YQSGISNAFKVSHSNGVTEGLNNKIKLIKRIAFGYRNFYNFRAR
                     IYLQQGLIFEN"
     gene            complement(553..1281)
                     /locus_tag="SCJ86_002102"
     CDS             complement(553..1281)
                     /locus_tag="SCJ86_002102"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding response regulator"
                     /protein_id="HEG1169632.1"
                     /translation="MLRIAIVDDDCTIVEGIRHYLLKKVPDIIIDHCYTDGDEAFKNL
                     QNQRFDLLITDITMGKISGLELIESLQQTSNRYFTSIVISAHQNFQYAKKGIDLSIKS
                     YITKPIDFPYLVKLVQEEKMRKSAANQNSLTDDSFNESIQQVIDYVQNNYSEKLTLKD
                     VATQFHYHPAYLGRAFKKETGYSFHDYLLDFRIKRASALLKDTFLSIDEISQKVGIDH
                     VSYFSVLFKKTTGHSPSNYRKKLL"
     gene            complement(1259..2833)
                     /locus_tag="SCJ86_002103"
     CDS             complement(1259..2833)
                     /locus_tag="SCJ86_002103"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289659.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine kinase"
                     /protein_id="HEG1169633.1"
                     /translation="MKNDLSKVIMRFTYILIFFITLLACFTLFFSYINVIEKEKNQNL
                     QNIQQITHYMETYNRILSGIVTEIYQNYDFQENLNDYYLSSPAEYMEDILSKDRSPTY
                     LVDNLKKLVQDDEVKNISISLDYEKYLSAYAPTKNEKISFIRSLSFISEDMPDATLIL
                     DVSINALKKSLFPTHYNYSLSNGIDSVNLSEDISPSKWKKTQQIAIGNHTLQVYENPN
                     YSSQTLLGLAIMIFLLMSLFILAIHQLLNRSFRNYIQQYQEIITSLNHSSTGDYKSIN
                     IENKVGDLKEIAMHINQFYLDNATYQKTLYENNWLKQEAKYAALQYQIEPHFLFNNLE
                     FIRMTAILNDDEEISDSIYLLSKLYRNNTYQPDIIDLKEELINIDLSLQLHQRRYRHN
                     FDYQLIDHIDQLMLPKLSLQPFVENYIKHGFDSSKKENRLTVYATETTNHYVLKIVEN
                     GKGMDQDKLEHIQQKLNDDIPYSENIGISNSFRRIKHLYPGDTRFVIESQLHVGTTIT
                     IKIQKGGNMHVENCNC"
     gene            3062..4000
                     /locus_tag="SCJ86_002104"
     CDS             3062..4000
                     /locus_tag="SCJ86_002104"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005877947.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HEG1169634.1"
                     /translation="MKLKKGLLGNLKKNRALFIMLLPGALWLFIFCYIPMFGVVIAFK
                     DFRISSRGFFTSLFNSPWVGWENFKYLFASQDAWIITRNTVVYNVVGILLSTFCTVSL
                     AIILSQMANKRLLKKMQTFMIFPNFLSWVVISYFVYAFLSTDKGLINILLSNFGVEPI
                     NWYSEPKYWPFIIVFTSLWKGVGYGSIIYYASIMGIDTSYYEAALVDGASKWQQIRNV
                     VLPHLRSIIVIMLILSVGSIFRADFGLFYQLPRNSGALYDVTNVLDTYVYRGLMNSGD
                     IGMSTAAGLYQSSVGFVLILITNKIVNKLDPSSALF"
     gene            4012..4923
                     /locus_tag="SCJ86_002105"
     CDS             4012..4923
                     /locus_tag="SCJ86_002105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289655.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HEG1169635.1"
                     /translation="MISKLKRQMYFSLPVERFFTILLILFTLSCIFPFIFVIVISLTD
                     EKALQLNGYELLPAQWSTDAYQYLIQDGGQLLRSLGVTIMITVIGTLITVFMTGTYAY
                     VLSRASFPYRKFFTFYLFFTMLFAGGMVPSYLVMTKMLGLKNTIWALILPLAFSPYNV
                     IILRTFFKKSIPESIIESAKMDGCSEFRVFFQIVLPLAIPGVATIGLFSSLGYWNDWF
                     NALLYIDTNKLIPLQYLLMKIQNSMEFLANNNDITLAQQQAIQNSLPQESTRMAMVVV
                     ATLPIAIVYPFFQKYFVQGLTIGGVKE"
     gene            4947..6419
                     /locus_tag="SCJ86_002106"
     CDS             4947..6419
                     /locus_tag="SCJ86_002106"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304876.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HEG1169636.1"
                     /translation="MKKNMQKYLIGGASVLTLGLLGACGSSNGEANNETGTTKVTWYQ
                     VGEEPKDFDEVMTKVNEKLKEEINVELDMRLIPDGDYQQKLGVMINSGEEYDICFVNG
                     TDYVNYGNKGAFISLNDHLDKELKDYAAELNEGFIEGGAINGETYGLPVNNDIATTEY
                     WTFNKDLVDQYQLDVENVEGVETLAPVLEKFAEKNTDTNITPIGMTRTSVCIPYDNII
                     GGGVPLAVAFEGDTQTVVNTYETPEMMTYLKAMHEFYKKGYILKDAATVTTDPFRIDS
                     KNWFLGRNSSGYQPLAQKKLEALAGHGLILKPAGKAFKTTGFARVAMHAVSASSKHPE
                     AALKLLNYVNTNQEVADLMVYGIEGKHYDRNEDGSITLTNSDDYYQGAWALVNTYLVS
                     KTSPITEESKKLEEESKKFQNEAPMSPILGFSFDASDFRTEIASVQNIVSQYEVLLSS
                     GTVDPEQYVPELLEKLEKAGLDKIQASAQEQYDEWRKSNK"
     gene            6470..7081
                     /locus_tag="SCJ86_002107"
     CDS             6470..7081
                     /locus_tag="SCJ86_002107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002352962.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF624 domain-containing protein"
                     /protein_id="HEG1169637.1"
                     /translation="MNENKWTKGFNWVFQLLLLNFTYFILICLGFGILGFFPATIALI
                     AGVVNRKKYGLQESVKFMSKIYKKNFVRYNLYCWSATLLLAGNITTIWLCWQQSAPLF
                     YLLSTLLSVITLFLVFAMLNWVVIKSLGLWMMGDRLIDYLLFTATMLPTVVMQGLVLG
                     IYFRILMILPVIFLFGGTAVTVILISKLTERALAKLPCQIQSH"
     gene            7111..9024
                     /locus_tag="SCJ86_002108"
     CDS             7111..9024
                     /locus_tag="SCJ86_002108"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304880.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="family 20 glycosylhydrolase"
                     /protein_id="HEG1169638.1"
                     /translation="MKLTDKQVANIAFIQQLEIPLTQKDEEQIDLIFTDTKANVRQIK
                     ITKNEQQRICLSYSHEDLLYRGLFICLPRFLAQGGEMPYIEETSIIETSYSLDASRNG
                     VMNKETLKKFISYLALMGYNTLYIYTEDTYEVEDLPYFGYLRGAYTKEEVNEIVEFAS
                     GFGIEVVPSIQTLAHLTQFLKWYAQQELMDDQNTLLIGEEKVYAAIEKMIIACKEMYQ
                     TERIHLGMDEAYQAGLGRYLSLYGYCDRTTLMIQHMDRVLSIVDKYKMKPMIWSDFIY
                     KLLDETGKDRYYNPDFKLKALAAKGLPAGLTYVHWDYGSDEVSSYEKVIDNHLKFCDI
                     DHYVLASGAHIWGRLAPNHGKTEAIVSAAVKASKNKGVRSVMLTTWGDEGQETEHWHS
                     LIGALKLADYVYSEEEWNDFEEIYDRLLGTGSYQLMSELRYFDEVDTITNNNLQMASV
                     SKLLLWQDPLVGIYDFHVAAHNKIATRTIGTYYHELAQKLDMNKNWNPKQPILTTIRQ
                     RYILLAEILAQKAELGIYLQHCCLVEDLAGLMELANRCEALANRVNELASIHEIIWYQ
                     TYKTYGWEVLEQRYAALAHRLKTVARRIKNHLQGQDALEELLEERLPFCQYQVPLEIS
                     GFNYRQSSVSGYN"
     gene            9152..12133
                     /locus_tag="SCJ86_002109"
     CDS             9152..12133
                     /locus_tag="SCJ86_002109"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 42"
                     /protein_id="HEG1169639.1"
                     /translation="MLSDYYQNLSVISVNTEPLRNYYIPYASKEEAKQELEREKTSQF
                     IDLNGTWDFQYYPSVQQLPENFFQEAFTESYDRISVPGVWQTEGFDLPQYINVSYPIP
                     FDPPYVPNDTPCGVYRRQFQIEQTDARFYLNLEGADSNCFVWINRNFVGYDQVSHSTS
                     EFDVTKYLQVGVNEIQILVMKWCDGTYFEDQDKWRMSGLFRPVYLLQRPTMHLTSYQL
                     RTLTDPKKGSGTLMVSFQRFTPIEVSLPISYELFHPDGRLIAKGECYSEQQINLDVED
                     CLFWTAETPKLYTLLLTCNGEYFKEKVGFRALTIKNRKLLLNGTPIKLYGVNYHDVNQ
                     QGTPVMTREQLRADLLLMKKLNVNTVRTAHYPKSPDFYELADEIGLYVINEADLECHG
                     VVNLYDTEANYQLIANDPTFEKPIMDRVIRSILPYQNRSSIIMWSMGNESGYGVNFEK
                     AQALARKLDDSRLIHNERSIEPVIDQVNDFSNLDVISQMYPPLEDVHRHCQRSDTEKP
                     FFMCEYSHAMGNGPGDLKTYFDLIQQYDSFLGGCVWEWTDHAINRGTLETPSYLYGGD
                     FGEKYHDGNFCVDGLLTPDRQLTVGALEYRNIHSPIQLIAVDEELQEVTLSNITAFTA
                     GSAFNLSLEWYIDGKKTVYEVMNPFILEPGKSCSLPITVPTTIHGAHTVAVSLSVYKN
                     DELYGQQQLFLKKEKMRLPRTAHHNDAPQISVKGTELIIKGESFIYIYQLAKAAFTSV
                     KINQKELLERPTQFSIWRAPTDNDRNLVNKWKAAGYAEMSLEIRKKDWKFVDDHLLIE
                     TSYRGVIPGLQPIFTMHIQLKINGAGVINQKVSVEKNPIFPDFPRFGMDWPLNDTFSK
                     VSYCGYGPYESYPDKHYAAMFGIYEMEIEEFDQPYIKPQEYGNRWQCDWVSIKNHQGS
                     TMTVYGNSNFSALPYSQAQLTEKSHHYELIKENRTWFNLSYKQNGIGSHSCGPYLEDR
                     FRFMEDTFDWNIVITFKGENR"
     gene            12130..13791
                     /locus_tag="SCJ86_002110"
     CDS             12130..13791
                     /locus_tag="SCJ86_002110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347193.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hyaluronate lyase"
                     /protein_id="HEG1169640.1"
                     /translation="MKETKINQVKKKLSRYQEVVYRNHCLKWSKNKLNVRFNGLFYRF
                     EQLEEVWKPVTNFQSCPVQLINDPKETYDLCITHLYNQHLKNDGFYLKVTNDNQLTIE
                     SANLRGFQYAMEALLESFFSVGDQLLLPELILKHEPSVKIRGIIEGFYGIPWTHEMRL
                     SLFSYMKDNQLNTFMYAPKDDELLRKKWRELYDAQELDKFKELLSAAEASNIDFWYLI
                     SPGNDIDITCEEDIQVLLKKLEQLIELGVFQFGLLMDDIDYQLKGAAKRRFREPAFAH
                     AYLVNRVEEYLSTRLSNHELVICPTEYDNRHGSRYLATLSQEIPEQLPFFWTGPSTLA
                     ASISTEELQEMASVYQRPMLIWDNIPVNDYLEDKELLFMSPYENRTPNLSKERYQVTG
                     VVSNPMAQLEASKFTINSMANYLWNCERFDPLETWTSVVEKVVGSKLQPAYLTLTNAF
                     PNHYTSSLLSDEELLLAKDPEWVTKEMAALVQAVKWIQQEQHTSRLRTELEPWLQAIT
                     ESWTFWQEWQLKKDPKEAEEWLAKKKTHRIGRDLVTAHLEQIIKS"
     gene            13831..14763
                     /locus_tag="SCJ86_002111"
     CDS             13831..14763
                     /locus_tag="SCJ86_002111"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005229489.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="haloacid dehalogenase-like hydrolase"
                     /protein_id="HEG1169641.1"
                     /translation="MVKRLISANYSDVKKMTSKELKQSIKASEGRVIVCETVANTLPQ
                     SGEITNAEVASAFGADLILLNGFDCYRPAVFGLPNVTIEKVFDLQQTIENPIPKLKEL
                     VGRPVGINLEPIAPKTNMMSEKVEISQGRASSVETIQKAEELGVDFICFTGNPGTGVT
                     NLEITKSVKMAKKYFSGLIIAGKMHSAGSEEPVVNKEAIISYAEAGADILLVPSVGSI
                     PGFLEQDLIEAVRLAKKYDLLTMSAIGTSQESARPETLRQLAIMNKSCGVDLQHIGDA
                     GYGGLAPAENIFETSVAIRGMRHTINCVSRSINR"
BASE COUNT         4894 a         2389 c         3030 g         4759 t
ORIGIN      
        1 gctaccaatc gggtatttca aatgccttta aggtgtccca ctcaaacggc gtcactgagg
       61 ggctgaataa caagatcaag ctgattaaac gaattgcgtt tggttatcgc aacttttata
      121 actttcgggc acggatctat ttacaacaag gtctgatctt tgaaaactaa aaaaatatgc
      181 ggcgcacaga acgcgccgca tatcggacta agttttattc aaatttgtct tcaccaacac
      241 gatttgacga agagcctatt taaataacta catccattta ttctatcttc taaaatagcc
      301 tttttcattt cagttaaata gttcagcgcc atttttgcac tcgacttgca tagtagtttt
      361 ttccatattc ctcaacctca gcgggggttg aagacataag gaaaatccta tgcgttatgc
      421 tcaactggat ctgattaaat attctaatgt atttttctct accatcatta aatggttaat
      481 agccaaaaat caccactttt caatggctcc ttgttgtata agtataacta gaagatgaac
      541 acgtctgaga aattatagaa gctttttacg gtaattactg gggctgtgtc cagtagtttt
      601 tttgaagaga acagaaaaat agctcacgtg atctattcct actttttgac taatttcatc
      661 aatagataag aatgtgtctt tcagtagagc acttgctctt ttgattcgaa agtctagcaa
      721 gtaatcatga aaggaataac ctgtttcttt tttgaaggcc cgtcctaaat aagcgggatg
      781 gtaatggaat tgtgtagcta catctttgag cgtaagcttt tcactataat tgttttgaac
      841 gtaatcaatc acttgttgaa ttgactcatt aaaagaatca tcagttagag agttttgatt
      901 agctgctgat ttacgcattt tttcttcttg tactaactta actaaataag gaaaatcaat
      961 gggtttcgtt atgtaacttt taatagataa atcaatcccc ttttttgcat actgaaaatt
     1021 ttggtgggca ctaatgacaa tactagtaaa gtagcgattg gatgtttgtt gcaaagattc
     1081 gattaattcg agtccagaaa ttttccccat cgtaatatct gtgataagta aatcaaagcg
     1141 ctggttttgc aggtttttaa atgcttcgtc tccgtctgtg tagcagtgat cgataatgat
     1201 gtcggggact ttcttcaaaa gataatgcct tatgccttct acaattgtac aatcatcgtc
     1261 aacaattgca attctcaaca tgcatattcc cccctttttg tattttgata gtaatcgttg
     1321 ttccgacgtg tagttggctt tctataacaa aacgtgtatc tccaggatag agatgcttta
     1381 tacgtcgaaa agaatttgag atgccgatat tctcactata aggaatatca tcattaagtt
     1441 tttgttgaat atgctccagc ttgtcttggt ccattccttt cccattttca acgattttta
     1501 acacataatg gttggttgtt tcagttgcat agacagtcaa acgattttcc ttttttgatg
     1561 aatcgaagcc gtgtttaata taattttcaa caaatggctg taaagaaagt tttggtaaca
     1621 ttaattgatc aatatgatca atcagctgat agtcaaagtt atgtcgataa cgacgttgat
     1681 gtagttgcaa ggagaggtcg atattaatta attcctcttt caaatcaatt atgtcgggct
     1741 gatatgtatt attgcgatac aacttgctca ataaatatat actgtcgctg atttcttcgt
     1801 catcattcaa tatggcagtc attcgaataa attccaaatt attaaataga aaatgaggct
     1861 cgatttgata ttgaagtgct gcatactttg cttcttgctt caaccaatta ttttcataaa
     1921 gtgttttttg atatgtagca ttatctaaat aaaactgatt gatatgcata gcgatttctt
     1981 tcaaatcacc gactttgttt tcgatattga ttgacttgta gtctcctgta gatgaatgat
     2041 ttaaggaagt aatgatttcc tgatactgct ggatatagtt acgaaaactt ctatttaata
     2101 attgatgaat agcaagaata aacaaactca ttaataagaa aatcatgatt gccagaccta
     2161 ataaggtttg tgacgaataa tttggatttt catatacttg taatgtgtgg ttaccgatag
     2221 ctatctgttg cgtttttttc catttagaag gcgagatgtc ttcagataaa ttgacggaat
     2281 caataccatt agacaaagaa taattgtaat gtgttggaaa gagactcttt tttagcgcgt
     2341 tgatcgaaac gtctagaatc aatgtagcat cgggcatgtc ctcactaatg aaacttaatg
     2401 aacgaataaa agaaattttt tcgttttttg taggtgcgta ggcacttaag tatttttcgt
     2461 aatctaatga gatagatata tttttaactt catcatcttg cacaagtttt tttaagttat
     2521 ctactaaata ggtaggggag cgatcctttg acaaaatatc ttccatgtat tcagcaggtg
     2581 atgagaggta gtagtcatta aggttttctt ggaaatcata attttgatag atctctgtca
     2641 cgataccgga gagaatacga ttatatgttt ccatatagtg ggtgatttgc tgaatgtttt
     2701 gcaggttttg atttttttct ttttcaatca cattgatata ggaaaagaac aatgtgaaac
     2761 aggcaagtaa agtaataaaa aaaatcaata tgtatgtaaa acgcataata acttttgata
     2821 aatcgttttt cattcaaagt cctcattttg ataatctcta attttatgaa tcattatagc
     2881 acatattaca agtgatgtaa acgctttgca aacagggcta cttatgattg tgaatacagt
     2941 ttccgtttaa aaactattct attgtagagg tttaataaac taaaaataat gtataaaaaa
     3001 ttaatgtaag cgattaacca agtgggtata atcaaggaaa cgctgagaga ggagagacaa
     3061 aatgaagcta aagaaagggt tattaggaaa tctaaaaaaa aaccgagcat tgtttattat
     3121 gcttttacca ggtgcgttat ggttatttat attttgctat ataccaatgt ttggagttgt
     3181 aatcgctttt aaagatttca gaatttcatc aaggggattc tttacaagtt tgttcaacag
     3241 tccttgggtt ggctgggaaa atttcaaata tctctttgct tcgcaagatg cttggatcat
     3301 tactcgcaat acggttgttt ataacgttgt aggtattttg ctaagtactt tttgtacagt
     3361 gtcattagca atcattctga gccaaatggc gaacaaacgg ttattgaaaa agatgcagac
     3421 atttatgatt tttcctaatt ttttatcatg ggtagtcatc agttactttg tttacgcatt
     3481 tttgagtacg gataaaggat taattaatat tttactaagc aattttggtg tggaacccat
     3541 aaattggtat agtgaaccga agtactggcc ttttattatt gtttttacga gtttatggaa
     3601 aggcgtaggc tatggatcca tcatttatta cgcgtcaatt atggggatcg atactagtta
     3661 ttatgaagct gctttagttg atggtgccag taaatggcaa cagataagga atgtagtctt
     3721 gccacattta agatcgatta ttgtcattat gttgattttg tctgttggta gtatttttcg
     3781 agctgatttt ggattgtttt atcaattgcc aagaaattcg ggggctcttt atgatgtgac
     3841 gaatgtatta gatacttatg tctaccgtgg attaatgaat agcggagata tcggaatgtc
     3901 tacggcagcg ggattatatc agtcatcagt tggctttgtc ttgattttga ttactaataa
     3961 gatagtcaac aagttagatc ctagttcagc actattttag gaggaatcaa aatgatatca
     4021 aaattaaaac ggcagatgta cttttcgctg ccagtcgaac ggtttttcac aatattacta
     4081 atcttattta ctcttagctg tatttttccc tttattttcg taatcgtcat ttcattaaca
     4141 gatgaaaaag cattacaact caacggttac gaattattgc cggctcaatg gagcactgat
     4201 gcatatcaat acttaataca agatggcgga cagttacttc ggtcattagg cgttacaatt
     4261 atgattacag tgattggaac gttgattact gtttttatga cgggaaccta tgcttatgtt
     4321 ttatcaagag cctctttccc ttaccggaag ttctttactt tttatctttt ttttaccatg
     4381 ttatttgcgg gagggatggt tccttcttac ttagtgatga cgaagatgct tggcttaaaa
     4441 aatacaattt gggctttgat tctacctctt gcttttagcc catacaatgt cattatttta
     4501 aggacattct ttaagaagtc gattccagaa tcgattatcg aatccgctaa aatggatggc
     4561 tgtagtgagt ttcgagtttt tttccaaatc gttttgcctt tagcgatccc aggggttgca
     4621 acaataggtt tattttcaag cttaggttat tggaatgact ggttcaacgc attgctgtat
     4681 attgacacca acaaattgat tccattgcaa tatttgttaa tgaagattca aaattcaatg
     4741 gaattcttag caaataataa tgacattaca ttagcgcagc aacaagcaat tcaaaatagc
     4801 cttcctcaag aatcaacacg aatggcaatg gtcgtagtag ctactttacc gattgcaatt
     4861 gtttatccgt tcttccaaaa atactttgtt caaggattga cgatcggtgg agtgaaagaa
     4921 taaaaaagaa taggagatgt taaaagatga aaaagaacat gcagaaatat ttgattggtg
     4981 gtgcttctgt actaaccctt ggcctattag gtgcttgtgg tagcagtaat ggcgaagcaa
     5041 ataatgaaac aggaaccaca aaagttactt ggtatcaggt tggagaagaa cccaaagatt
     5101 ttgatgaggt catgacaaaa gtcaacgaaa aattaaagga agaaatcaat gtagagttgg
     5161 atatgcggct gattccggac ggggattatc agcaaaagct tggtgtgatg atcaattcag
     5221 gagaagaata tgatatttgt ttcgtcaatg gcacagatta tgtgaattat gggaacaaag
     5281 gtgctttcat tagtctcaac gatcatttgg ataaagagtt gaaagattat gcagctgaat
     5341 tgaatgaagg atttattgaa ggtggggcaa ttaatggcga aacttatgga ttgccagtca
     5401 ataacgatat tgccacaacg gaatattgga cctttaataa agatttggta gatcaatacc
     5461 aattggatgt agagaatgtt gaaggtgtag aaacattggc tccagtatta gaaaagtttg
     5521 cagaaaaaaa tactgatacg aatattacgc caatcggtat gacccgtacc agtgtctgta
     5581 ttccttatga caatattatc ggtggtggtg taccgctagc ggttgcattt gaaggcgata
     5641 cacaaacagt tgttaatact tatgagactc cagaaatgat gacttatttg aaggctatgc
     5701 atgagtttta taaaaaagga tatattttga aagatgcagc cactgttacg acagatccat
     5761 ttcgaattga tagtaaaaac tggtttttag gtcgtaactc aagtggttat caaccattag
     5821 cacaaaaaaa attagaagct ctagcgggac atggattaat tttaaaacca gctggaaaag
     5881 cttttaaaac gactggattt gcaagagtgg caatgcatgc ggtatctgct tcaagtaaac
     5941 atccggaagc agccttgaaa ttgttgaatt atgtgaatac caatcaagaa gtagcggatt
     6001 taatggttta tggaattgaa ggaaaacact atgatcgcaa tgaagatggt tcgattactt
     6061 taacaaactc tgatgattat tatcaaggcg cttgggcatt ggtaaatact tatctagttt
     6121 caaaaacttc accgattaca gaagaaagta aaaaactaga agaagaaagt aaaaagtttc
     6181 aaaatgaagc accgatgtct cctattttag gtttctcttt tgacgcttct gattttcgaa
     6241 cagaaattgc aagtgtacaa aatatcgtgt cacaatatga agtactttta agctcgggaa
     6301 cagttgaccc tgaacaatat gtaccagagt tgttagaaaa actagaaaaa gctggattgg
     6361 ataaaattca ggcatctgct caagaacaat acgatgaatg gcgaaaatct aacaaataat
     6421 tttttactaa atgaaaagaa tagactaagc agttagtggg gggattcaaa tgaacgaaaa
     6481 caaatggacc aaaggattta attgggtttt tcaattgttg ttactaaact ttacttattt
     6541 tatactaatt tgtttaggat ttggaatttt aggttttttc cctgctacga tagctttgat
     6601 tgctggcgtt gtgaatcgaa aaaaatatgg cttgcaagag tctgtgaagt tcatgagtaa
     6661 aatctataag aaaaattttg ttagatataa tctatattgc tggtcagcaa ctttgttgtt
     6721 ggccggcaat attactacga tctggctttg ttggcagcaa agcgcaccat tgttttattt
     6781 attaagtaca ttattatcgg taattacact gttcctagtc tttgcaatgt tgaattgggt
     6841 cgtgatcaaa agtcttgggt tgtggatgat gggagaccga ctgattgact atttgctttt
     6901 cacagcaacg atgttgccaa cggtagtcat gcaaggcttg gtgcttggaa tttatttccg
     6961 aatattgatg attttgcctg tgatttttct gtttggcgga acagctgtaa cggtaatatt
     7021 gattagtaaa ttaactgaaa gagcgttagc aaagttgcct tgtcagatac aatcgcacta
     7081 attttttcac aaaaaggagt atttctttct atgaagttaa ctgataaaca agtagctaat
     7141 atagccttta tccagcaact tgagattcca ttgacacaaa aagatgaaga acaaatagac
     7201 ttgattttta ctgacacaaa ggctaatgtg cgacaaatca agattactaa aaatgagcag
     7261 caacgaattt gcttgtctta ttcacatgaa gacttgctgt atcgaggatt gtttatctgc
     7321 cttccacgtt ttttagctca aggaggagag atgccataca ttgaagaaac ctcgatcatt
     7381 gagaccagtt attcattaga tgcttctcgt aatggcgtga tgaataaaga gactctaaaa
     7441 aaattcattt cttacttagc gttgatgggg tacaacacat tgtatattta cacagaagat
     7501 acctatgaag tagaagacct tccatatttt ggttaccttc gcggagcgta tacaaaagaa
     7561 gaagtgaatg aaattgttga atttgcttct ggttttggca tagaagtagt tccatcgatc
     7621 cagacccttg ctcacttgac ccaatttttg aagtggtatg ctcaacaaga actgatggat
     7681 gatcaaaata ctctactgat cggtgaagaa aaggtttatg cggcaatcga aaagatgatt
     7741 attgcctgca aagaaatgta tcagacagag cgtattcatt tgggaatgga tgaagcctat
     7801 caagcaggtc tgggacgata tttaagcctg tatgggtatt gtgatcggac aactttaatg
     7861 attcaacata tggaccgtgt gttatcaatt gttgataaat ataagatgaa accaatgatt
     7921 tggagtgatt tcatttataa gctattagat gaaaccggta aagataggta ttacaatcct
     7981 gattttaaac tcaaagcatt agcagctaaa ggattaccag caggattaac ctatgttcat
     8041 tgggactatg gttcagatga agtttcctcc tatgagaaag taattgataa ccatttgaaa
     8101 ttttgtgaca ttgatcatta cgttttagct agtggtgccc atatctgggg gagactagcc
     8161 ccgaatcatg ggaaaacaga agcaattgtt tctgcagctg taaaagcttc taaaaataaa
     8221 ggtgtacgtt ctgtgatgtt aactacttgg ggagatgaag ggcaggaaac agagcattgg
     8281 cattccttaa ttggggcatt aaaattagca gattatgtct attctgaaga agagtggaat
     8341 gattttgagg aaatctacga tcgtttatta ggaacaggta gttatcaatt aatgtccgaa
     8401 ttacgatatt tcgatgaagt ggataccatt acaaataaca acttacaaat ggcttccgtg
     8461 agtaaactgc tactttggca agatccgtta gttggtatat atgactttca tgtagccgct
     8521 cataacaaaa ttgccactag aacaattggt acttattatc atgagttagc tcagaaatta
     8581 gatatgaata aaaattggaa tccaaaacaa ccaattttaa caactattcg tcaacgatat
     8641 atacttttag ctgaaatttt agcacaaaag gcagaactag gcatctattt acaacactgc
     8701 tgtctggtag aagaccttgc aggattaatg gaattagcga atcggtgtga agcactagcc
     8761 aatcgagtaa atgaattggc atctattcat gaaataattt ggtatcaaac atacaaaacc
     8821 tatggttggg aagtattaga acaacgatat gctgcattag ctcatcgtct gaaaaccgtt
     8881 gcgcgtcgta ttaaaaatca tcttcagggg caagatgccc tagaggagct tttggaagaa
     8941 cgattaccgt tttgtcaata tcaagtgccg cttgaaattt ctggctttaa ttatcggcaa
     9001 agctcggtgt caggctacaa ttaacgaagg aatcttcccg ttgaacggac cacttgctca
     9061 tctaatggga aagtggttcg ttttctattc ataagtagct taccactaac tcttttatta
     9121 agaagagaaa aaaagtagga agtgagaaaa tatgttatcg gactattatc aaaatctgtc
     9181 agttataagt gtcaatacag aaccattacg taattattat attccttatg caagtaaaga
     9241 agaagcaaaa caagagttag aaagggaaaa aacgagccag tttatcgatc tcaatggtac
     9301 atgggatttt cagtattatc cttctgtcca acagttaccg gaaaattttt ttcaagaggc
     9361 ctttactgaa agctatgata gaatttctgt tcctggagtt tggcaaacag agggatttga
     9421 cttacctcag tatatcaatg tatcttatcc aattcctttt gatccgcctt atgtacctaa
     9481 tgatacccct tgtggtgtat atcggcgtca gtttcaaatt gagcaaacag atgcacgctt
     9541 ttacttaaat ctagaaggag cagatagtaa ttgttttgtt tggataaata gaaactttgt
     9601 aggctatgac caagtgagtc atagtacatc tgaatttgat gtgaccaagt atttacaggt
     9661 tggggttaat gaaattcaaa ttctagtgat gaagtggtgt gatggtacct actttgaaga
     9721 tcaggataag tggcgaatga gcggtctatt tcgtccagtg tatcttcttc aacgaccaac
     9781 tatgcatctg acaagttatc aactacggac gttgactgat cctaaaaaag gaagcggaac
     9841 attaatggtt tcttttcaac gatttactcc tattgaagta tcgttaccta tttcttacga
     9901 attattccat cctgatggac gtctcatagc gaaaggggag tgttactcgg aacaacaaat
     9961 aaatttggat gttgaagatt gccttttttg gacagcagaa acgcctaaat tatatacatt
    10021 acttttaaca tgtaatggtg aatattttaa agaaaaagtt ggctttcgag cactgacgat
    10081 caagaatcgt aagttattgc tgaacggaac acctattaaa ttatatggag tcaactatca
    10141 tgacgtgaat cagcaaggca cgccagtgat gacccgagag cagttacgtg cagatttgtt
    10201 attaatgaaa aaattaaatg tgaatactgt acgaacggca cattacccta aatcaccaga
    10261 tttttatgaa ttggcggatg agattggtct gtatgtaatc aatgaagcag atttggagtg
    10321 tcatggtgtg gttaatctat atgatactga agcgaattat caactaattg ctaatgatcc
    10381 gacattcgaa aagccgatta tggaccgagt gatccgttct attcttcctt atcaaaatcg
    10441 aagctctatc attatgtggt ctatggggaa tgaatctggt tatggagtaa attttgaaaa
    10501 agctcaagct ttagcacgga aattggatga ttcacggttg attcataatg aacgctcgat
    10561 tgaaccagtt attgaccaag tgaatgattt tagtaacttg gatgttatta gtcaaatgta
    10621 tcccccactg gaagatgttc atcgtcattg tcaaagaagt gatactgaaa aacctttttt
    10681 tatgtgtgaa tatagccacg ctatgggcaa cggtcctgga gatcttaaaa cgtacttcga
    10741 tttgattcaa cagtatgatt cctttcttgg cggttgtgtc tgggagtgga ctgatcatgc
    10801 aatcaataga ggaactttag aaacgccatc ttatttatac ggtggtgatt ttggtgaaaa
    10861 atatcacgac ggcaattttt gcgtagatgg tttattgacc ccagatcggc aattaacagt
    10921 aggtgcattg gagtaccgta atattcattc gcctattcaa ctgattgctg tagatgagga
    10981 gctgcaagaa gtaaccttga gcaatataac tgcttttaca gccggttcag catttaacct
    11041 ttctcttgaa tggtatatag atggaaaaaa aacagtttat gaagtaatga atcctttcat
    11101 acttgagcct ggcaaaagtt gttcattgcc catcacagta cctacaacga ttcatggtgc
    11161 tcatacggtt gctgtttcgc tatctgttta taaaaatgat gagctttatg gacagcaaca
    11221 gctctttctt aaaaaagaaa aaatgagatt gccaagaact gctcatcata atgatgcacc
    11281 acaaatttca gtaaaaggca cagagctgat aatcaaagga gaatccttta tttatatata
    11341 tcaattagca aaagctgcgt tcacttctgt aaagatcaat caaaaggaac ttttggaacg
    11401 tcctactcaa ttttcaattt ggcgtgcacc aacggataat gatcgtaact tagtaaacaa
    11461 atggaaagca gctggatatg ctgaaatgtc tttagaaatc agaaaaaaag actggaaatt
    11521 cgtagatgat catctcctga tcgaaacgtc ttatcgagga gttattccag gattacagcc
    11581 aatttttaca atgcacatac agttgaaaat aaatggtgca ggcgtaatca accaaaaagt
    11641 atcagtggag aaaaatccga tttttccaga tttccctcgc tttgggatgg actggccgtt
    11701 gaatgatact ttttcgaagg tttcttattg tggctatggg ccgtatgaaa gttatcctga
    11761 taagcactat gctgcgatgt ttggtattta tgagatggag attgaggaat ttgatcagcc
    11821 atatatcaaa ccacaagaat atggtaaccg ctggcaatgt gattgggtat caatcaaaaa
    11881 tcatcaagga tctaccatga cggtttatgg aaactctaat ttttctgctc tgccttatag
    11941 tcaagctcaa ttaaccgaaa agtcacacca ttatgaatta ataaaagaaa atagaacttg
    12001 gttcaattta agttataaac aaaatggtat cggctcacac agttgcggtc cttatttaga
    12061 agatcgtttc cgttttatgg aagatacttt tgactggaat atagtgatta ctttcaaagg
    12121 agaaaataga tgaaagaaac aaagatcaat caagtcaaaa aaaaattatc tcgttatcaa
    12181 gaagttgttt atcgaaacca ttgcttaaaa tggtcaaaaa ataagctgaa tgttcgtttt
    12241 aatggattat tttatcgttt cgaacagtta gaagaagtct ggaaaccggt aacgaatttc
    12301 caatcatgtc ctgtacagct cataaatgat ccaaaagaaa catacgattt atgtataaca
    12361 cacctttata accagcattt gaaaaatgac ggattttatt taaaggttac taatgacaat
    12421 caacttacga ttgaatcagc aaaccttcga gggtttcaat atgctatgga agccttatta
    12481 gagagttttt tttcggtagg agatcaatta ttattaccgg aattgattct gaaacatgaa
    12541 ccatcagtga aaattcgagg aatcattgaa gggttttatg gtatcccctg gacacatgaa
    12601 atgcgcctct ctcttttttc atatatgaaa gataaccaat tgaatacttt tatgtatgcc
    12661 cctaaagatg atgaattatt acgtaaaaaa tggcgtgaac tctacgatgc gcaagaatta
    12721 gacaaattta aggagctttt atctgctgct gaggctagca atatcgattt ttggtactta
    12781 attagtcctg gaaatgatat tgatattacg tgtgaagaag acattcaagt tcttctgaaa
    12841 aagttagaac aactgattga gcttggcgta tttcaatttg gtctattaat ggatgatatt
    12901 gactatcaat taaaaggtgc tgctaaacgc cgttttcgag agccagcatt cgctcatgcc
    12961 tatttagtga atcgagtaga ggaatatctt agcactcggt tgtccaatca tgaattagtc
    13021 atttgtccta cagaatatga taatcgtcat ggttctcgct atttagcaac gttaagtcaa
    13081 gaaataccag aacaattacc gttcttttgg actggcccaa gcacgttagc ggcgtcaatt
    13141 tctacagaag agctgcagga aatggcttct gtctatcaac ggccgatgct gatttgggat
    13201 aatattccgg taaatgatta tttagaggat aaagaactat tgttcatgag tccatatgag
    13261 aatcgaacac cgaatctttc taaggagaga taccaggtta caggagtagt ttcgaatcca
    13321 atggctcaat tagaagcttc aaagtttacg atcaatagta tggctaatta cttatggaac
    13381 tgtgagcgtt ttgatccttt ggaaacttgg acgtcagtcg ttgaaaaagt agttggaagt
    13441 aaactacaac cagcttatct taccttaacc aatgcttttc caaatcatta cacaagcagt
    13501 ctgctttcag atgaagaatt actgctagcc aaagacccag aatgggtcac aaaagaaatg
    13561 gctgctttag tccaagcagt aaaatggatc cagcaagaac aacatacatc tagattgaga
    13621 acagaattgg agccttggtt acaagcaatc acagaaagtt ggactttctg gcaagaatgg
    13681 caattaaaaa aagatccaaa agaagcagaa gaatggttgg ctaagaaaaa aacacaccgc
    13741 ataggacgtg atttagtaac tgcccattta gagcaaatta tcaaaagtta gatatcttaa
    13801 aaaataaaaa gcaagaaaaa ggagaacaaa atggtcaaga gattaattag tgcaaactac
    13861 agtgatgtca agaaaatgac gtcaaaagag ttgaagcaat ccatcaaagc gagtgaagga
    13921 cgggtgattg tttgtgaaac ggttgctaat actcttccac aatctggaga aattacgaat
    13981 gcagaagttg cgtcagcatt tggtgcagat ttgattcttt taaatggttt cgattgttat
    14041 cgtcctgcag tttttggact accaaatgtg acgattgaga aggtttttga tctacagcaa
    14101 acgattgaaa acccgattcc taaattaaaa gaactagtcg gtcgtcctgt gggaattaat
    14161 ttagaaccca ttgcccctaa aacaaatatg atgagtgaga aagtagaaat cagccaagga
    14221 agagcaagct ctgttgaaac gatacaaaaa gcagaagaac taggagtcga ttttatttgc
    14281 tttaccggta atccaggcac tggagtaacc aatttagaaa ttactaaatc cgtaaaaatg
    14341 gcaaaaaagt atttttcggg attgattatt gctgggaaaa tgcatagcgc cggctcagaa
    14401 gagccagttg tgaataaaga ggcgattatt tcttatgcgg aagcaggcgc agatatttta
    14461 ctagttcctt cagtagggtc tatcccaggc tttttagagc aagacctaat tgaagcggtt
    14521 cggttggcaa aaaaatatga tttgttaacg atgtctgcaa tcggtactag tcaggaaagt
    14581 gcccgaccag aaacattgcg gcaattagcc attatgaata aaagttgtgg tgttgattta
    14641 caacatattg gagatgctgg atacggggga ttggcaccag cagaaaatat tttcgaaacc
    14701 tcggttgcaa ttagaggaat gcgacatacc atcaactgcg ttagccgctc gatcaatcgc
    14761 taacaaaatt aaataaaaaa tggttaaatt gattatttaa gtgggaacat acttgagata
    14821 tcagctaact tctgtcactt taaaaaaggg actgtgacag aagtgaaaat agacaaaaaa
    14881 tatccgaaca agagactagg atagcagaaa aactatcgtt ttgcaacgag gaccaactgg
    14941 accgtaggag caattctctt attgttcgga tatttcgctt gttcaaggac ttatgtctgt
    15001 agtgcccttt tttggtagga aaaatggtag ggaaccatat catttttaat gaaaatcaag
    15061 catttcaaaa gt
//
LOCUS       DARBET010000062        14344 bp    DNA     linear   BCT 07-NOV-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4027
            SAMEA114569841-rid20112183.denovo.064, whole genome shotgun
            sequence.
ACCESSION   DARBET010000062 DARBET010000000
VERSION     DARBET010000062.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA114569841
            Sequence Read Archive: ERR12203666
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 14344)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14344)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001972618.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001972618.1
            Genome Coverage       :: 132x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/07/2023 13:18:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,041
            CDSs (total)                      :: 2,975
            Genes (coding)                    :: 2,908
            CDSs (with protein)               :: 2,908
            Genes (RNA)                       :: 66
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 67
            CDSs (without protein)            :: 67
            Pseudo Genes (ambiguous residues) :: 0 of 67
            Pseudo Genes (frameshifted)       :: 26 of 67
            Pseudo Genes (incomplete)         :: 28 of 67
            Pseudo Genes (internal stop)      :: 20 of 67
            Pseudo Genes (multiple problems)  :: 6 of 67
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14344
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA114569841-rid20112183.denovo.064"
                     /isolate="VRE4027"
                     /db_xref="taxon:1352"
                     /country="Denmark"
     gene            110..491
                     /locus_tag="SCJ86_002112"
                     /pseudo
     CDS             110..491
                     /locus_tag="SCJ86_002112"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303242.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            622..1572
                     /locus_tag="SCJ86_002113"
     CDS             622..1572
                     /locus_tag="SCJ86_002113"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002821555.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium transporter CorA family protein"
                     /protein_id="HEG1169642.1"
                     /translation="MLKTYSLTKDTLLVSSESNIKNSSVVYVYGDEREYIESSEHKYD
                     FKIGNILTFDDRVAYDTMIDKNDEKSLLVSFLFPEVHEGGHLDTLTTSVVFLVFKNKL
                     IIFTKQKLKFIPELLSNMVLRDVNHFVEEVLLKIMQKDFYMMLNDLRGVKEKIDDLDS
                     EISTSGSLKPTFGDLLTLQKYMIALSTTYKANHKALDFIKKNFTTIDDIDFSTKKIID
                     DLYDTSDTMDRIILSYSQYLDKLENMINNMISYQLNMIMKTLTEISIVLTIPAIIFGF
                     WGINVHVPFEKSSYGVLAVLIISAILSLICWLLMRRKTYL"
     gene            2090..2545
                     /locus_tag="SCJ86_002114"
     CDS             2090..2545
                     /locus_tag="SCJ86_002114"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303240.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HEG1169643.1"
                     /translation="MVQQIVLPIKDSSVLKMVQDTLLDSFRAGRRNYTVFQVGKATLL
                     RVSDVMTLKKSDVYNPDGSVKNATFIHDKKTGKANTLYLKPVQQDLLQYHDWLAQENI
                     NSEWLFPSTAHYDLHITEKQFHKVMAHVGDLLGINLEDKEKKLRVRKPF"
     gene            2886..3488
                     /locus_tag="SCJ86_002115"
     CDS             2886..3488
                     /locus_tag="SCJ86_002115"
                     /inference="COORDINATES: protein motif:HMM:NF013878.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEG1169644.1"
                     /translation="MLDPLFTDETFITFGFGHLVELAEPGHYDEKWQNWKLESLPIFP
                     DRYDFEVAKDKGKQFKIVAELLKKANTIIVATDSDREGENIAWSIIHNANAFSKDKTF
                     KRLWINSLEKDVIRSGFQNLQPGMNYYPFYQEAQTRQIADWLIGMNASPLYTLNLQQK
                     GVQGTFSLGRVQTPTLYLIFQRQEAIENFKKEPFFEVEAK"
     gene            3582..3914
                     /locus_tag="SCJ86_002116"
     CDS             3582..3914
                     /locus_tag="SCJ86_002116"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002308085.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="topoisomerase I"
                     /protein_id="HEG1169645.1"
                     /translation="MGNQEGIIADVQTKEKKTNSPSSFSLSSLQSKVNQLYKATASQT
                     LKAMQGLYEAKLLSYPRTDTPFITENEFAYLKANFGKYSGFLGLDLEMLLSLIMIRSN
                     NSTQILTN"
     gene            complement(4065..5044)
                     /locus_tag="SCJ86_002117"
                     /pseudo
     CDS             complement(4065..5044)
                     /locus_tag="SCJ86_002117"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332307.1"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
     gene            complement(5049..5816)
                     /locus_tag="SCJ86_002118"
     CDS             complement(5049..5816)
                     /locus_tag="SCJ86_002118"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007209218.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR/GlpR transcriptional regulator"
                     /protein_id="HEG1169646.1"
                     /translation="MNKKRRLEKILDMLKIDGTITIKEIIDELDISDMTARRDLDALE
                     ADGLLTRTHGGAQLLSSKKPLEKTHIEKKSLNTKEKIDIAKKACSLIKDGDTIFIGPG
                     TTLEQLALELKGRKGYKIRVITNSLPVFLILNDSETIDLLLLGGEYREITGAFVGSMA
                     STNLKAIRFAKAFVSANAVTHNSIATYSDKEGVIQQLALNNAVEKFLLVDSTKFDRYD
                     FFNFYNLDQLDTIITDNQISPQHLEEFSQYTTILKAD"
     gene            6306..6731
                     /gene="lacA"
                     /locus_tag="SCJ86_002119"
     CDS             6306..6731
                     /gene="lacA"
                     /locus_tag="SCJ86_002119"
                     /EC_number="5.3.1.26"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005237726.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactose-6-phosphate isomerase subunit LacA"
                     /protein_id="HEG1169647.1"
                     /translation="MAIVVGADLKGTRLKDVVKNFLVEEGFEVIDVTKDGQDFVDVTL
                     AVASEVNKDEQNLGIVIDAYGAGPFMVATKIKGMVAAEVSDERSAYMTRGHNNSRMIT
                     VGAEIVGDELAKNIAKGFVNGKYDGGRHQIRVDMLNKMC"
     gene            6748..7263
                     /gene="lacB"
                     /locus_tag="SCJ86_002120"
     CDS             6748..7263
                     /gene="lacB"
                     /locus_tag="SCJ86_002120"
                     /EC_number="5.3.1.26"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012027025.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactose-6-phosphate isomerase subunit LacB"
                     /protein_id="HEG1169648.1"
                     /translation="MRIAIGCDHIVTDEKMAVSEFLKSKGHEVLDFGTYDHARTHYPI
                     YGKKVGEAVVSGQADLGVCICGTGVGINNAVNKVPGVRSALVRDMTSAIYAKEELNAN
                     VIGFGGKITGGLLMNDIIEAFIEAEYKPTEENKKLIAKIEHVETHNAHQADEDFFTEF
                     LERWDRGDYHD"
     gene            7274..8206
                     /gene="lacC"
                     /locus_tag="SCJ86_002121"
     CDS             7274..8206
                     /gene="lacC"
                     /locus_tag="SCJ86_002121"
                     /EC_number="2.7.1.144"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290553.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tagatose-6-phosphate kinase"
                     /protein_id="HEG1169649.1"
                     /translation="MILTVTLNPSVDISYPLETLKIDTVNRVKDVSKTAGGKGLNVTR
                     VLYESGDKVTATGFLGGKIGEFIESELEQSPVSPAFYKISGNTRNCIAILHEGNQTEI
                     LEQGPTISHEEAEGFLDHYSNLIKQSEVVTISGSLPSGLPNDYYEKLIQLASDEGVAV
                     VLDCSGAPLETVLKSSAKPTAIKPNNEELSQLLGKEVTKDIEELKDVLKESLFSGIEW
                     IVVSLGRNGAFAKHGDVFYKVDIPDIPVVNPVGSGDSTVAGIASALNSKKSDADLLKH
                     AMTLGMLNAQETMTGHVNMTNYETLNSQIGVKEV"
     gene            8209..9189
                     /gene="lacD"
                     /locus_tag="SCJ86_002122"
     CDS             8209..9189
                     /gene="lacD"
                     /locus_tag="SCJ86_002122"
                     /EC_number="4.1.2.40"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290551.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tagatose-bisphosphate aldolase"
                     /protein_id="HEG1169650.1"
                     /translation="MVLTEQKRKSLEKISDKNGVISALAFDQRGALKRLMAQYQDTEP
                     TVAQMEELKVLVADELTKYASSMLLDPEYGLPATKALDKEAGLLLAYEKTGYDTSSTK
                     RLPDCLDVWSAKRIKEQGADAVKFLLYYDVDSSDELNQQKQAYIERVGSECVAEDIPF
                     FLEILAYDEEISDAGSVEYAKVKPRKVIEAMKVFSDPRFNIDVLKVEVPVNVKYVEGF
                     ADGEVVYSKAEAADFFKAQEEATNLPYIYLSAGVSAKLFQETLQFAHDSGAKFNGVLC
                     GRATWAGSVEPYIKEGEKAAREWLRTTGFENIDELNKVLVKTASPWTDKV"
     gene            9217..9534
                     /locus_tag="SCJ86_002123"
     CDS             9217..9534
                     /locus_tag="SCJ86_002123"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011669100.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS lactose/cellobiose transporter subunit IIA"
                     /protein_id="HEG1169651.1"
                     /translation="MNREEMTLLGFEIVAYAGDARSKLLEALKAAENGDFAKADSLVV
                     EAGSCIAEAHNSQTAMLAREASGEELPYSVTMMHGQDHLMTTVLLKDVIHHLIELYKR
                     GAK"
     gene            9534..11240
                     /locus_tag="SCJ86_002124"
     CDS             9534..11240
                     /locus_tag="SCJ86_002124"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290549.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HEG1169652.1"
                     /translation="MHKLIELIEKGKPFFEKISRNIYLRAIRDGFIAGMPVILFSSIF
                     ILIAYVPNAWGFHWSKDIETFLMTPYSYSMGILAFFVGGTTAKALTDSMNRDLPATNQ
                     INFLSTMLASMVGFLLMAAEPAKEGGFLTAFTGTKGLLTAFIAAFVTVNVYKVCVKNN
                     VTIRMPEEVPPNISQVFKDLIPFTVAVVLLYGFELIVKGTLGVTVAESIGTLLAPLFS
                     AADGYLGITLIFGAYAFFWFVGIHGPSIVEPAIAAITYANIDANLHLIQAGQHADKVI
                     TSGTQMFIVTMGGTGATLIVPFLFMWICKSERNRAIGRASVVPTFFGVNEPILFGAPI
                     VLNPIFFVPFIFAPIVNVWIFKFFVDTLNMNSFSANLPWVTPGPLGIVLGTNFQVLSF
                     ILAGLLVVVDTIIYYPFVKVYDEQILEEERSGKTNDALKEKVAANFNTAKADAVLGKA
                     GVAKEDVAANNNITKETNVLVLCAGGGTSGLLANALNKAAAEYNVPVKAAAGSYGAHR
                     EMLPEFDLVILAPQVASNFDDMKAETDKLGIKLAKTEGAQYIKLTRDGQGALAFVQQQ
                     FD"
     gene            11382..12788
                     /gene="lacG"
                     /locus_tag="SCJ86_002125"
     CDS             11382..12788
                     /gene="lacG"
                     /locus_tag="SCJ86_002125"
                     /EC_number="3.2.1.85"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290548.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-phospho-beta-galactosidase"
                     /protein_id="HEG1169653.1"
                     /translation="MTKTLPKDFIFGGATAAYQAEGATHTDGKGPVAWDKYLEDNYWY
                     TAEPASDFYHKYPVDLELAEKYGVNGIRISIAWSRIFPTGYGEVNEKGVEFYHNLFAE
                     CHKRHVEPFVTLHHFDTPEVLHSDGDFLNRENIEHFVDYAAFCFEEFPEVNYWTTFNE
                     IGPIGDGQYLVGKFPPGIQYDLAKVFQSHHNMMVSHARAVKLYKDKGYKGEIGVVHAL
                     PTKYPYDPENPADVRAAELEDIIHNKFILDATYLGHYSDKTMEGVNHILAENGGELDL
                     RDEDFQALEAAKDLNDFLGINYYMSDWMQAFDGETEIIHNGKGEKGSSKYQIKGVGRR
                     IAPDYVPRTDWDWIIYPEGLYDQIMRVKNDYPNYKKIYITENGLGYKDEFVDNTVYDD
                     GRIDYVKKHLEVLSDAIADGANVKGYFIWSLMDVFSWSNGYEKRYGLFYVDFDTQERY
                     PKKSAHWYKKVAETQVIE"
     gene            12811..13083
                     /locus_tag="SCJ86_002126"
     CDS             12811..13083
                     /locus_tag="SCJ86_002126"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007209209.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3884 family protein"
                     /protein_id="HEG1169654.1"
                     /translation="MLSDTLEKVQHLFRPGFEKVYIVSFEDCPMIPELEATPLLKCGK
                     WYVSTGKEWICHSDLELSAFEWEFLQSLDVEIRETIHFEVNYLPFQ"
     gene            13104..14003
                     /locus_tag="SCJ86_002127"
     CDS             13104..14003
                     /locus_tag="SCJ86_002127"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002889672.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldose 1-epimerase family protein"
                     /protein_id="HEG1169655.1"
                     /translation="MTIELKNEYLTVQFKTLGGQLTSIKDKDGIEYLWQADPNYWNGQ
                     APILFPICGSLRNDWAIYRPQERPFFTGLIRRHGFVRKEEFILEEVNDNSVTFSIKPN
                     AEMLDNYLYQFELRVVYTLNGKSIRTEFQVTNLEMEKTMPYFIGAHPAFNSPLVEGEN
                     YEDYYIEFSEVESCSIPKSFPETGLLDLQDRTPFLENQKILDLDYSLFSHDAITLDQL
                     KSRSVTLRSRKSGKGLRVDFDDFPNLILWSTTNKSPFIALEPWSGLSTSLEEGNFLED
                     KRQVTKISPQETSRKIYDITILN"
BASE COUNT         4686 a         2363 c         2765 g         4530 t
ORIGIN      
        1 atgacactat cgttcttcat caacgtaagt tagatttgtt gaaagagcag aaaaaaggat
       61 ttctacaaaa aatgtttgtt tgatatttta aatagaaaag agagataaag tgaagttttt
      121 aaattttttg aactataaat atttaaactg ggtattatat ggtattacat tattttttat
      181 tatttattac ttttttaccg atgatgatat ttttttaatt ttagcaatac tttttcttgt
      241 ttcatctaat tttagtgata acttaattga agttcttaca gaaccagaaa ttcggtctat
      301 gacggatgaa gaacaacaga ttattaaagc tatgatttcg gaaaataaga aagacgtagt
      361 agttatcaaa aagattagag agtatacaaa tttaggtgtt ttacaagcca aaaaagtata
      421 tgaaaaaaaa aaaaagggtc tataattaca aattagataa tacattagaa gctggagaca
      481 agattgtcta gataatgtgt gagataaaat ttttggcagg ttatcataca taagacaaaa
      541 tgtttttaaa actgataacg tatttattcc acaacaatta cgcatttatt tctgagaaaa
      601 atataaagca ggaggattac aatgctgaaa acttattcct taacaaaaga tacattatta
      661 gtttcgtcag aatcaaatat caaaaattct tctgttgttt atgtttatgg tgacgaacga
      721 gaatatatcg aatcttctga acataaatat gactttaaaa taggcaatat actaacgttt
      781 gatgatcgag ttgcatatga cacgatgata gataagaacg atgaaaaaag tttgctcgta
      841 agttttttgt ttcccgaggt acatgaaggt gggcatctag ataccctgac gacatctgtt
      901 gttttcttgg tatttaaaaa taagttgata atttttacaa aacaaaaatt aaaattcatt
      961 cccgaactct tatccaatat ggttttacgt gatgtcaatc atttcgtgga ggaagtactt
     1021 ttaaagatca tgcaaaaaga tttctatatg atgcttaacg accttcgtgg agtcaaggaa
     1081 aaaattgatg atttagactc agaaatatca acgtccggtt cactcaaacc aacgtttggt
     1141 gatttattga ctcttcaaaa atacatgata gctttgtcaa caacttataa agctaaccat
     1201 aaagcgcttg atttcattaa aaaaaatttc acaactattg acgatattga ttttagtact
     1261 aaaaaaataa ttgatgattt atacgataca agtgatacaa tggatagaat cattttaagt
     1321 tacagccaat atttggacaa attggaaaat atgataaaca atatgatttc gtatcagcta
     1381 aacatgatta tgaaaaccct aaccgagata tcaattgttt tgactattcc agcaattatt
     1441 tttggctttt ggggaatcaa tgtccatgtc ccgtttgaaa agtcttctta tggtgtttta
     1501 gccgtattga ttatctctgc tatactaagt ctaatttgct ggcttttgat gcgacgcaaa
     1561 acatatttgt aaagtatttg tgatacccta atatgattta aagcagaatt tctttgttga
     1621 ctttttaaaa ggctgattcc tttcaataat ttaatagctt cagcggctgc tcagaagtca
     1681 ataaattgga ctatttttat tcagttactt aggcaacatt tgagatacga ttgcgattat
     1741 aaaaattgat ataccaacca atggctgctt ggaagactga catattagcc tcgatatcat
     1801 cgaataacta aatacagaaa aatacaataa atattatcaa actagggact atactagaaa
     1861 acatcagtac attccttaac tcagggtcta taattagaaa atcgcccctt tgaaaactga
     1921 ttaaaacagc ccccattatc tagtgactag ataatggggg ctgttttttt gttaagtgat
     1981 ataataaacc ataaaagaag tttagaaata cggtcctggt catcaataaa taaacaattt
     2041 aattaattat ttatttaatt atttaatttc agaaaggtat acatgaatca tggtgcaaca
     2101 aattgtccta cccatcaaag actccagcgt cttaaagatg gtacaagata cacttctcga
     2161 tagtttccgt gctggtcgcc gaaattacac cgtttttcaa gttggtaagg ccacgctact
     2221 acgtgtgagt gatgtcatga cattaaaaaa atcagatgtc tataatccag acggctctgt
     2281 caaaaatgca acctttattc atgataaaaa gaccggtaaa gcaaacacgt tatatttaaa
     2341 gcctgttcag caagacttgt tgcaatatca tgattggctg gcccaagaaa atatcaattc
     2401 tgagtggtta tttccctcga cagcccatta tgatcttcat atcactgaga agcagtttca
     2461 taaggtgatg gcgcatgttg gtgatctctt aggaatcaac ttggaagaca aagagaaaaa
     2521 attgcgagtc agaaaaccgt tttgagatat gaacagcatt tgccttcacg ccttagcgat
     2581 tttaaataat agccttgaaa gtgattatgg aaaagccctc tacgctcaat gcaaaattga
     2641 cgtagaaact atttttgatc gattgaaagg tgtatttggt atgtgaagaa cccatgtcag
     2701 aggcaagaac gttgcaccta ctgacattgg aatgatgctt atgagcatga atctaaccaa
     2761 attatccctt gaggctagaa gaaaagcgga agcttttcaa cacaaagcag ccaaaaataa
     2821 aaaacgcgac gaagcaatca catttatgat tattcatcgc gttttttata tttagagcta
     2881 gttatttgct agacccacta tttacagatg aaacgtttat cacctttggt tttgggcatt
     2941 tagtggaatt agcagaacca ggtcattatg acgaaaagtg gcaaaattgg aaacttgaat
     3001 ctttgccgat ttttcctgat cgatacgatt ttgaagttgc aaaagataag ggaaagcagt
     3061 ttaaaattgt tgcagaactt ctcaaaaagg caaatacaat tattgttgca acagatagcg
     3121 acagagaagg tgaaaatatc gcctggtcga ttatccataa tgcaaatgcc ttttcaaaag
     3181 ataaaacatt taaaagacta tggatcaata gcttagaaaa agatgtgatc cgaagcggtt
     3241 ttcaaaattt gcaaccagga atgaattact atccctttta tcaagaagcg caaacacgcc
     3301 aaattgccga ttggttgatc ggcatgaacg caagcccttt gtatacgtta aatttacaac
     3361 agaagggcgt acaaggtaca ttttcactag gacgtgttca aacgcccacc ttatacctta
     3421 tttttcagcg ccaggaagcc atagagaatt ttaaaaaaga accttttttc gaggtggaag
     3481 ctaagtaaaa caagggtcat ttaagggcgt tctaagcccc acacagcgtt ttaaaaccca
     3541 agaggagctt tttagctttt gtttcttcta aacaagctaa aataggcaat caagagggga
     3601 taattgctga tgttcaaacc aaagagaaga aaacgaatag tccgagttcg ttttctttaa
     3661 gtagtttgca atcaaaagtc aatcagcttt ataaagcgac agcgagccaa actttaaaag
     3721 ctatgcaagg actgtatgaa gcaaaattat tgagttatcc aagaacagat acaccattta
     3781 ttacagagaa cgaatttgct tatttaaaag cgaattttgg caaatatagc ggttttttag
     3841 gacttgatct tgaaatgttg ttatcattga taatgatacg ttcaaacaac agcacccaaa
     3901 ttttgacgaa ttagtgaaac tctatgaaaa agacgtagta aaacatgcaa catcctattc
     3961 taatcagctg gtaaagctta attaggttat ataagcttga aaataaaggg tttgtccaat
     4021 atggacaagc ctttttctta tgttttgcca cagatttgcc acacttattg tgtaagagta
     4081 tcaaggatag gtataatttg atactttgtt tttttagtga tgtgcaaata aatttctgtt
     4141 acacgactac ctctagaatg ccctacacgg tcttgaattg cttctagggg tattccttac
     4201 tctgctaaga aactaatatg cgtatgacgg aatatatggg tcgtaatatg tttattaaaa
     4261 ctaagtttat tatcaatatc aattcctgct cgttttggat taccttttcg atttaatttt
     4321 ccattttcag gtgagttgag gaaattgtta aggacactat aatgaagagg tgttccatta
     4381 tagtcgggtt tatgacgact tttggaagta aaaatccatc cattatcttt ataatcagga
     4441 ttgaatctca tatgttggtg atttaactca atttcctctt taagaatttc tatagccctt
     4501 ttagataatt ggacatttcg gtaactttgt aagtttttta cattaccttc atagctattt
     4561 tctgcagctc ctccgtgagt ggactgacta atgatgatat tttgcttttc aaaatattga
     4621 taggcaccaa tattttggag tttcgactct gtaagtcaac agggccatta gttagaatac
     4681 ctattgggat gcggttttta tgtagagcca gcagtagttg atgtatctca ggaatcatcg
     4741 taatctgatc gagttcatat tcatagtcag cttggaagtc tagagcttct gtcctagaaa
     4801 tactatcgat gccaaattct tccatagtct tctgacagcg aaaaattcta agctgctcaa
     4861 tactgatgag ttttttcgtg tatttaggaa aagcaacatc agaccaatat ctgaaacgtt
     4921 tgtaaatgtt atctatttgt tggatatcaa tcgttggaaa acatttctca acgctacgtt
     4981 taaaggggag ttgatggtca taaagagtgt catcaaggtc aaaaacaata tttttataag
     5041 tcataattct agtccgcttt taaaatagta gtgtactggc taaattcctc taagtgttga
     5101 gggctaatct ggttatctgt aatgatggta tcgagttgat ctagattata gaagttaaag
     5161 aaatcgtatc gatcgaattt agtactgtct actaataaga atttttctac agcattgttt
     5221 agggcaagtt gttgaatcac accttccttg tcactatatg tagcaataga attatgggta
     5281 acagcatttg cactaacaaa agctttggca aatctaattg cttttaaatt tgtcgaagcc
     5341 attgaaccta caaaagctcc agttatttct ctatattcac cgccaagaag caataaatca
     5401 atggtttcgc tatcatttag aatcaagaac acagggagac tatttgtaat gacacgaatt
     5461 ttataacctt tacgaccttt caattctaat gccagttgtt caagtgtagt tccaggtcca
     5521 ataaaaatag tatcgccatc tttgattaaa gagcaggctt ttttagcaat gtcaattttt
     5581 tcttttgtat ttagactttt cttctcgata tgtgtctttt caagtggctt tttagaggaa
     5641 agcaattgtg caccaccatg agtacgtgtt aaaagtccat cagcttctag agcatcaagg
     5701 tctctacggg ctgtcatatc ggaaatatct agttcatcta ttatttcttt tatggttatg
     5761 gtcccatcaa tctttaacat atctaaaatt ttttctaatc gtcgtttttt gttcatatga
     5821 agacccttct ttcataaagt aatttttttc caaagataat tctcttttaa ttgtatcata
     5881 aaagacaata ttttcaaggt aaaacaaaca atttcaaaca aaaacaaatg ttagatgatg
     5941 aaataagaac agaggattga cgtatattag cttaggtcag attttgtata agacgaaaat
     6001 aaagtaggac ctcttaatca gtaagttata gaaagtaaaa gacttttgta atacttgaat
     6061 agatatttca cgtccatttt gtgatggatt aaatgaacaa aaatgaacaa taatttaacg
     6121 gtgttatcta ttttttaaaa aaacaaataa aaaaaacaaa aaataaacaa aaaaagtgtt
     6181 gctttttgtt tgaatgcttg atattatata aacaaagaaa tgatgaaaac gttttttcga
     6241 acattttgca aaatgttttc tacttctacg tagcatttct ttctaaaatt taggaggtgg
     6301 tcaaaatggc tattgttgtt ggtgcagatc tcaaaggtac gagattaaaa gatgttgtga
     6361 aaaattttct tgtagaagaa ggttttgagg taattgatgt gactaaggac ggacaagatt
     6421 ttgttgatgt taccttggca gttgcctctg aagtaaataa agatgagcaa aacttaggta
     6481 ttgtaattga tgcttatgga gctggtccat tcatggtagc aactaaaatt aaaggtatgg
     6541 ttgcagcaga agtttcagat gaacgctcag cttatatgac acgtggacat aacaattcac
     6601 gaatgattac ggttggcgct gaaatcgtcg gtgatgagct tgctaaaaac atcgctaaag
     6661 gtttcgtcaa tggtaaatat gatggcggac gtcatcaaat ccgagtagat atgcttaaca
     6721 aaatgtgtta agaaaaggag ttaaggaatg agaatcgcaa ttggatgtga ccacattgtc
     6781 acagatgaaa aaatggcagt atcagaattc ttgaaatcaa aaggacatga ggtccttgat
     6841 ttcgggacat atgaccacgc acgtactcac taccctatct atggtaaaaa agtcggtgaa
     6901 gcagtagtaa gtggtcaagc agacttggga gtatgtatct gtggtacagg tgtcggcatt
     6961 aacaatgcag tcaataaagt tccaggtgtt cgctcagcgt tggttcgtga tatgacatca
     7021 gctatatatg caaaagaaga gttaaatgcg aatgttatcg gtttcggtgg aaagattact
     7081 ggtggtcttc ttatgaatga catcattgaa gctttcattg aagctgagta caaaccaaca
     7141 gaagaaaata aaaaattgat tgcaaaaatc gaacacgttg aaacacataa tgcacatcaa
     7201 gcagatgaag atttctttac agaattcctt gaaagatggg accgtggtga ctaccacgat
     7261 taaggtataa caaatgattc tgacagtcac actaaatcct tcagtagaca tctcttatcc
     7321 tctagaaaca ttgaaaattg atacagtgaa tagggtgaaa gatgttagta agacagctgg
     7381 tggaaaaggc ttaaatgtta ctagagtctt gtatgaatct ggtgataaag ttactgctac
     7441 aggtttctta ggtggtaaaa ttggtgaatt tattgaaagc gaattagaac aatctcctgt
     7501 tagtccagct ttttataaaa tctcaggaaa cactagaaac tgtattgcta tcttacacga
     7561 gggtaaccaa acagaaattt tggaacaagg accaacaatt tctcatgaag aagcagaagg
     7621 ctttcttgac cactatagca atcttattaa acaatcagaa gttgtgacaa tttcaggaag
     7681 tttaccttca ggacttccca acgattatta tgaaaaactt atccaacttg cttcggatga
     7741 aggagtagca gtcgttttgg attgttcagg tgcacctctt gaaacagttc taaaatcgtc
     7801 ggctaaacct actgccatca aacctaataa tgaggaactt tcacagttgc ttggaaaaga
     7861 agtaacaaaa gatattgaag aactcaaaga cgttcttaaa gagtcacttt ttagtggtat
     7921 cgaatggata gtggtctcat taggtcgaaa cggtgccttt gcaaaacatg gtgatgtttt
     7981 ctataaggta gatattcctg atatcccagt tgttaatcca gtcggatcag gggactcaac
     8041 ggtagctggt attgcatcag ctttaaatag taaaaaaagt gacgctgact tattaaaaca
     8101 tgcgatgaca ttgggtatgt taaatgctca agaaacaatg acagggcatg tgaatatgac
     8161 taattacgaa acactaaact cacaaattgg agtaaaagag gtataaaaat ggtacttaca
     8221 gaacagaaac gcaaatcttt ggaaaaaatt tcagataaaa acggtgttat ctcagctttg
     8281 gcatttgacc aacgtggtgc tttgaaacgt ttgatggcac agtatcaaga tacagaacca
     8341 actgtagctc aaatggaaga actaaaggtt ttggttgctg atgaacttac aaaatacgct
     8401 tcttcaatgt tgcttgaccc tgaatatgga cttccagcaa caaaagcatt ggataaagaa
     8461 gcaggtcttc ttcttgccta tgaaaaaaca ggttatgata catcaagtac aaaacgtttg
     8521 cctgactgct tggatgtttg gtctgcaaaa cgtatcaaag agcaaggcgc tgatgccgtt
     8581 aagttcttgc tttactatga tgtagacagc tcagatgaac ttaaccaaca aaaacaagct
     8641 tatattgaac gtgttggttc tgaatgtgtc gctgaagata ttcctttctt cttggaaatc
     8701 cttgcttatg atgaagaaat ctcagatgca ggttctgttg aatatgcaaa agtgaaacct
     8761 cgcaaagtca ttgaagcgat gaaagttttc tctgatccac gctttaacat tgatgttctt
     8821 aaagtagaag taccagttaa cgttaaatat gtcgaaggtt ttgctgatgg tgaagtagtt
     8881 tacagtaaag ctgaagctgc tgacttcttt aaagcacaag aagaagcgac taaccttcca
     8941 tacatctacc taagtgcagg tgtatctgct aaattgttcc aagaaacatt gcaatttgct
     9001 cacgactcag gtgccaagtt taatggtgtg ctttgtggac gtgctacttg ggcaggatct
     9061 gtagaacctt acatcaaaga aggtgaaaaa gcagcacgtg aatggttgcg tactactgga
     9121 ttcgaaaata ttgatgaact taacaaagtt cttgttaaaa cagctagtcc atggactgat
     9181 aaagtatagt aaaaacataa aacggaggat attgttatga acagagaaga gatgactctc
     9241 ttagggtttg aaattgttgc ttatgctgga gatgctcgct ctaaactttt agaagcgctt
     9301 aaagcggctg aaaatggtga tttcgctaag gcagatagtc ttgtagtaga agcaggaagc
     9361 tgtattgcag aggctcacaa ttctcagaca gctatgttgg ctcgagaagc ttctggggag
     9421 gaacttccat acagcgttac catgatgcat ggtcaggacc acttaatgac tactgtctta
     9481 ttaaaagatg tgattcatca cctcatcgaa ctttataaaa gaggagcaaa ataatgcata
     9541 aactcattga acttattgag aaagggaaac cgttctttga gaaaatctct cgaaacatct
     9601 atcttcgtgc tattcgtgat ggatttatcg ctggtatgcc agtcatcctt ttctcatcta
     9661 tctttatcct tattgcctat gtcccaaatg cttggggatt ccactggtca aaagacattg
     9721 agaccttctt gatgactcct tatagctatt cgatgggtat cctagcattc tttgttggtg
     9781 gtactaccgc taaagctttg acggactcaa tgaaccgtga cctacctgcg accaatcaga
     9841 ttaacttctt atctacgatg ctagcgtcta tggtagggtt ccttttgatg gcagctgagc
     9901 ctgcaaaaga aggaggcttc ttgacagcct tcacgggaac aaaaggtctt ttgacagcat
     9961 ttattgcagc ctttgtgaca gttaatgttt acaaggtctg tgtgaaaaat aatgttacta
    10021 ttcgtatgcc tgaagaggtt ccaccaaata tctctcaagt atttaaagac ttgattccat
    10081 tcactgtagc ggttgttctc ctttatggat ttgaactcat tgttaaggga actcttggtg
    10141 taaccgttgc agaatcaatc ggtacccttc ttgctcctct cttctcagct gcagatggtt
    10201 accttggaat tacacttatc tttggtgctt atgccttctt ctggtttgtt ggtatccacg
    10261 gtccttcaat tgtcgaacca gcaatcgctg ctatcactta tgccaatatc gatgccaact
    10321 tgcatcttat ccaagctgga caacatgcag acaaagttat cacttctggt actcaaatgt
    10381 ttattgttac catgggtgga acaggagcta cattgattgt tccattcttg ttcatgtgga
    10441 tttgtaaatc agaacgtaac cgtgccatcg gacgtgcctc agttgttcca acattctttg
    10501 gggttaatga gccaatctta tttggtgcac caatcgtatt gaatccgatt ttctttgtac
    10561 cgttcatttt cgctccgatt gtcaacgttt ggatctttaa attctttgtt gacacattga
    10621 acatgaactc attctctgcc aaccttccat gggttactcc tggtccgttg ggaattgtac
    10681 tcggtactaa cttccaagtt ctatcattta tcttagccgg actcttggta gttgttgata
    10741 ctatcattta ttacccattt gttaaggtat acgatgaaca aattcttgaa gaagaacgtt
    10801 ctggtaaaac aaacgatgct cttaaagaaa aagtagctgc aaacttcaat actgctaaag
    10861 cagatgccgt tcttggaaaa gcaggcgttg ctaaagaaga tgtagcagca aacaataata
    10921 tcacaaaaga aactaatgtc cttgttcttt gtgcaggtgg aggtacaagt ggcttgcttg
    10981 ccaatgcctt gaacaaagca gcagcagaat acaatgttcc agtcaaagcg gcagcgggta
    11041 gttacggtgc tcaccgtgaa atgttgccag agtttgactt ggttatcttg gctccacagg
    11101 tcgcttcaaa cttcgatgat atgaaggctg aaacggataa attgggcatt aaacttgcaa
    11161 aaactgaagg agctcaatat atcaaattga cacgtgatgg acaaggtgct cttgcatttg
    11221 ttcaacaaca gtttgattaa taaatttgta aaagttattt gagacgagat tattctttca
    11281 attccttata atattgttaa ccatatcatt gcatttcatt tccttggttt ccggaactca
    11341 gaatatcgtc tcaaatgctt tttttgaaag gatttatagt tatgactaaa acacttccta
    11401 aagattttat ttttggtggt gctacagctg cttatcaagc agaaggtgcg actcacacgg
    11461 atggtaaagg cccagttgca tgggataagt atcttgaaga caattattgg tacacagcag
    11521 agcctgcaag tgatttttac cataaatatc ctgttgattt agaattagct gaaaaatatg
    11581 gcgttaatgg tattcgtatt tcaattgcct ggtctcgtat tttcccaact ggctatggcg
    11641 aagtaaatga aaaaggtgtt gaattctacc ataatctttt tgccgagtgc cacaaacgtc
    11701 atgttgagcc attcgtgact ttgcaccact ttgacacacc agaagttctt cactcagatg
    11761 gagatttctt aaaccgcgaa aatatagaac actttgtaga ttatgccgct ttctgttttg
    11821 aagagttccc agaagtaaac tactggacaa ctttcaatga aattggccca attggagatg
    11881 gtcaatactt ggttggtaaa ttccctccag gcattcaata tgatcttgcc aaagtcttcc
    11941 aatcacacca caatatgatg gtttctcatg cacgtgctgt gaaattgtat aaagataaag
    12001 gttataaagg cgaaattggt gtagtacatg cgttgccaac caaataccct tatgatccag
    12061 aaaatccagc agatgttcgt gccgctgagt tagaagatat catccataac aaatttatct
    12121 tggatgcaac ctaccttgga cactattcag ataaaacgat ggaaggggtc aaccacatcc
    12181 tagctgaaaa tggtggagaa cttgatcttc gtgatgaaga cttccaagca cttgaagcag
    12241 ctaaagattt gaacgatttc cttggtatca actactacat gagtgattgg atgcaagcct
    12301 ttgatggtga gactgaaatc attcacaatg gtaagggtga aaaaggaagt tctaagtacc
    12361 agattaaagg tgttggtcgc cgaatcgcac cagattatgt gcctagaacg gattgggact
    12421 ggattattta tcctgaaggc ttgtatgacc aaatcatgcg agtgaaaaat gattatccaa
    12481 actacaagaa gatttacatc actgaaaacg gtcttggata caaagatgag tttgtagata
    12541 ataccgttta tgatgatggt cgtattgatt acgtgaagaa acacttggaa gttttatcag
    12601 acgcaattgc ggatggtgca aatgttaaag gttacttcat ctggtcactt atggatgtct
    12661 tctcatggtc aaacggatac gaaaaacgtt atggactatt ctatgtagac tttgacacgc
    12721 aagaacgcta tcctaagaaa tcagctcatt ggtataagaa agtagcagaa actcaagtga
    12781 tagagtaata ttacttgaaa atgtaaaaaa ttgttatccg atactttaga aaaagtacaa
    12841 cacttattta gaccaggctt tgaaaaggta tatattgtta gtttcgaaga ttgtcctatg
    12901 attcctgagc ttgaagctac cccgttgcta aaatgtggta aatggtatgt ttcaactgga
    12961 aaagagtgga tttgccattc tgatttagag ttatcagcat ttgaatggga atttttacaa
    13021 tcactcgatg tagaaatacg tgagactatt cacttcgagg taaattattt gcctttccaa
    13081 taataaaaag aaaggaatta gttgtgacta tcgaattaaa aaacgagtat cttactgttc
    13141 agtttaaaac acttggtggt caactgactt cgattaaaga caaggatggg atagagtatc
    13201 tctggcaagc tgatccaaat tattggaatg gacaggcgcc tattctattt cctatctgtg
    13261 gtagtttacg aaatgattgg gctatttata gacctcaaga aagacccttt tttacaggtc
    13321 ttattcgtag acatggtttt gttcgaaaag aagaatttat tctggaagaa gtcaatgata
    13381 atagcgtgac ctttagtatt aaaccaaatg ctgagatgct tgataattac ctatatcagt
    13441 ttgaactgag agttgtttat accttgaatg gtaaatcaat tagaaccgaa tttcaggtaa
    13501 cgaacttgga aatggaaaaa acaatgcctt actttattgg agcccatcca gcattcaata
    13561 gtcctttagt agaaggtgaa aactatgagg attattatat tgaatttagt gaggttgagt
    13621 cttgctcgat accaaagagt tttcctgaga caggattatt agatttgcaa gatcggacac
    13681 cttttttgga aaatcaaaaa attctcgatt tggactactc acttttttcc catgatgcca
    13741 ttacacttga ccagctcaag tctcgtagcg taacgcttcg gtcaaggaag tctggtaaag
    13801 gtcttcgagt agattttgat gattttccga acttgattct gtggtctaca actaataaaa
    13861 gtccattcat tgctttggaa ccttggagtg gtctctccac ttcacttgaa gaaggtaatt
    13921 tcttagaaga taaaagacag gtaacgaaaa tttcacctca agaaacttct agaaaaatct
    13981 atgatattac tatccttaat taaaatttgt tattatttga gtcttcaata agatttgaat
    14041 actcttacct tgtgatgata gattggtctt ctccgtgcca gtcttacatc gcaaggtttt
    14101 taagtttttt gtgggtctat gtcaactgta gtgggtgacg aaaagctaat atctaaagac
    14161 agtctggtag taaaaaataa gattatttac tacttcttga tttaaaataa tagtggagcc
    14221 attaatttgt ggcaaattta atttaatatt ttgcctcaaa ttagccacac tttttataga
    14281 actaagagga aaaatgaaat tttcttccta ttaatatagt tgtttgaaat cttaaatata
    14341 gcgt
//
LOCUS       DARBET010000063        13983 bp    DNA     linear   BCT 07-NOV-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4027
            SAMEA114569841-rid20112183.denovo.065, whole genome shotgun
            sequence.
ACCESSION   DARBET010000063 DARBET010000000
VERSION     DARBET010000063.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA114569841
            Sequence Read Archive: ERR12203666
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 13983)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13983)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001972618.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001972618.1
            Genome Coverage       :: 132x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/07/2023 13:18:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,041
            CDSs (total)                      :: 2,975
            Genes (coding)                    :: 2,908
            CDSs (with protein)               :: 2,908
            Genes (RNA)                       :: 66
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 67
            CDSs (without protein)            :: 67
            Pseudo Genes (ambiguous residues) :: 0 of 67
            Pseudo Genes (frameshifted)       :: 26 of 67
            Pseudo Genes (incomplete)         :: 28 of 67
            Pseudo Genes (internal stop)      :: 20 of 67
            Pseudo Genes (multiple problems)  :: 6 of 67
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13983
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA114569841-rid20112183.denovo.065"
                     /isolate="VRE4027"
                     /db_xref="taxon:1352"
                     /country="Denmark"
     gene            complement(224..604)
                     /locus_tag="SCJ86_002128"
     CDS             complement(224..604)
                     /locus_tag="SCJ86_002128"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335490.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S1 RNA-binding domain-containing protein"
                     /protein_id="HEG1169656.1"
                     /translation="MTYKIGQIIEGKVTGIQPYGAFVSLDNETQGLIHVSEVQSGYTK
                     NIHSLLKVGQPVTVQIIDIDEYSKKISLSLRTLEKTNPAVPYRRKRYFTNKNRKIGFT
                     TIAEQLPIWIEEALSELERKENKK"
     gene            668..1861
                     /locus_tag="SCJ86_002129"
     CDS             668..1861
                     /locus_tag="SCJ86_002129"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629534.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal phosphate-dependent aminotransferase"
                     /protein_id="HEG1169657.1"
                     /translation="MTGGLKMIEFDKQISRKDTESVKWDAIAETYQMDDLLPLWVADM
                     DFLSPGGVAKAFSEYIKHGIFGYATLSDKLYQAIIHWERQHHAVELTKENIVFTSGVL
                     TSLAAAVQTFTKPGDSILIHDPVYPPFSSIIETNQRHIVRSSLLEKNNHFIMDLSDME
                     KKIEENNVKAMILCNPHNPGGRVWSKEELKQLSELCLKHQVILFSDEIHQDLTLFDHT
                     FTSMLTIDPNLDDLLVAFTSATKTFNLAAIKNSMVFIKNPEMKADFEKHLVMNQQHEI
                     NTFGLIGTQAAYETGGEWLSQLIPYLEKNVQTVNQFFEQYLPKVRIMNPEGTYLMWLD
                     FTAYISDDQELENTLVRKGKVVLNPGITFGPSGHGHMRLNIACPEETLLEGLNRIKYA
                     LETID"
     gene            complement(1938..2522)
                     /locus_tag="SCJ86_002130"
     CDS             complement(1938..2522)
                     /locus_tag="SCJ86_002130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005876718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HEG1169658.1"
                     /translation="MAFPQLDLKSEKGPKAVIKTNRGDITVQLFPELAPKTVQNFIEL
                     SKKGYYDGVIFHRVIPDFMIQGGDPTGTGMGGESIYGESFEDEFSRELFNLRGALSMA
                     NSGPNTNGSQFFIVNNTNVPANMLGQLEGAGFPSEIIEAYKGGGTPWLDFRHTVFGHV
                     LEGMDTVDEIANVQRGPQDRPVHDVVIETIEISE"
     gene            2723..3721
                     /locus_tag="SCJ86_002131"
     CDS             2723..3721
                     /locus_tag="SCJ86_002131"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296073.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HEG1169659.1"
                     /translation="MEIYDITIIGAGPVGMFAAFYAGMRQAKTKIIDSLPQLGGQLAT
                     LYPEKYIYDIPGYPAIKASELIDQLEKQLTTFNHTFHLKEEVLSLTREDEVIEITTNK
                     GIHYSKAVILALGNGSFQPRKLNLDNAESFENHGLDYFVNDLMKYAGKKVAIAGGGDS
                     AIDWTLMLEPIASEVYLIHRRPEFRAHEHSVSRLKSSSVNLLTPYLIDGLSGNNGELT
                     DIRLKKVKSDETIDLMIDSLIVNYGFSSNLEHLSSWGLDSTRNAITVKSDMSTSIPGV
                     YAAGDICTYEGKVKLIATGLGEAPTAVNNALHYINPKERTQPGHSTSLYDKNLRPS"
     gene            3833..4342
                     /locus_tag="SCJ86_002132"
     CDS             3833..4342
                     /locus_tag="SCJ86_002132"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287163.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidylglycerophosphatase A"
                     /protein_id="HEG1169660.1"
                     /translation="MVVKTETLEEKARELLKERGVTVEDIAQLVMFLQKDYISDLTLA
                     DCTESVNSVLTKREVHNAIITGIQLDILAEKKKLMSPLQHIIEDDEGLYGIDEILALS
                     IVNVYGTIGFTNYGYIDKVKPGILKELNSHDGVHVHTFLDDIVGAIAAAAASRLAHKE
                     PEKRSRLTQ"
     gene            complement(4372..5031)
                     /locus_tag="SCJ86_002133"
     CDS             complement(4372..5031)
                     /locus_tag="SCJ86_002133"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339768.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR01906 family membrane protein"
                     /protein_id="HEG1169661.1"
                     /translation="MKNSRWQWMEYAGLFSLFLTLISLAVGVTINFRPLYVFDIGHLQ
                     ILDYTSLDQETLLKNFDHLMNYLNNPFKTILSLPDFPVSASGAHHFYEVKILFLVDYA
                     VFFITLIPSILFIKYLQKNDRLWRLIRPFQIGMLLPVVFGFFMMIGFDRFFILFHETF
                     FNNDDWLFDPVTDPIINVLPEEFFMHSFILFFVLLELFFAVFLFLGKNSLKQTKKKEL
                     V"
     gene            complement(5033..5797)
                     /locus_tag="SCJ86_002134"
     CDS             complement(5033..5797)
                     /locus_tag="SCJ86_002134"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR01457 family HAD-type hydrolase"
                     /protein_id="HEG1169662.1"
                     /translation="MDYKGYLIDLDGTIYRGTQPIPAGKRFVEELQKRKLPFLFVTNN
                     TTKSPETVANRLADEFDIHVAPETVYTATLATIDFMKADGKGKKVYVIGEAGLIDLIL
                     AAGFTWEEEAPDYVVVGLDNYLTYEKVVKATLAIQKGATFIGTNPDKNIPTERGLLPG
                     AGSVISFVETATQTPPIYIGKPEAIIMDKAVEVLGLQKEEVIMVGDNYETDIQAGIRN
                     NIDTLLVLSGFTKEEDVPGLPIPATYVKQSLDEWSF"
     gene            complement(5805..6482)
                     /locus_tag="SCJ86_002135"
     CDS             complement(5805..6482)
                     /locus_tag="SCJ86_002135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287156.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YutD family protein"
                     /protein_id="HEG1169663.1"
                     /translation="MSAKQNEQQNVEHKTEIAKKKSSAEQETTVTEELTAALEEIVEE
                     PKAEKVKGELVTLLDDSNFLIGERHYRLVSDYREGFNAEKLGERYSDVLARYDYIVGD
                     WGYEQLRLKGFFQADNRRAHPDQRIDSLEDYLYEYCNFGCAYFVIERIGGKKEKTTQR
                     RKKKKNHNRAYIDEKKGPVASNRKKPVIKNRKTESAKTSEKVKKQEQPKQVEKTKTAF
                     TIRKREE"
     gene            complement(6519..7904)
                     /locus_tag="SCJ86_002136"
     CDS             complement(6519..7904)
                     /locus_tag="SCJ86_002136"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase"
                     /protein_id="HEG1169664.1"
                     /translation="MEKIVILHTNDLHSHLENWPRIRRFLDQRKRENEKKEKTTTIAV
                     DLGDFVDRWHPLSEATNGQENILLMNEVGYDAATIGNNEGVGNSKEELNHLYDHAEFD
                     IILDNLFDKNTLQPPAWTKPYKIITTKHQTKIGLIAFTAPFPLTYNPNGWDIRNPYDI
                     LPELVEDLRSQVDVLVLMSHLGIQDDFQIAKEIPAIDVILGSHTHHLFREGHVINDVQ
                     IAAAGKYGQYVGEVHLTIDEEKKILKHSAKAIPTETMTAFTEDEQEINSYLERGHELL
                     KEKEVADLPFSLSLDIFNEHSFIQVAFEAVKERGKTEAAILNSGLFLTEIPKGRVNQD
                     QLHTALPHPMHLLNVTLGGNDLIRLVLEIEKNRNFLRNYPMKGMGFRGKIFGQMVYNG
                     ITYDSINHQVFWKNKPIDLNKNYTFTTVDHLMFVPFFPTIEIAGKNEFLFPEFIRSVV
                     GDYLKAHYPIK"
     gene            complement(8114..8198)
                     /gene="ffs"
                     /locus_tag="SCJ86_002137"
     ncRNA           complement(8114..8198)
                     /ncRNA_class="SRP_RNA"
                     /gene="ffs"
                     /locus_tag="SCJ86_002137"
                     /product="signal recognition particle sRNA small type"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00169"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00169"
     gene            complement(8221..8736)
                     /gene="tadA"
                     /locus_tag="SCJ86_002138"
     CDS             complement(8221..8736)
                     /gene="tadA"
                     /locus_tag="SCJ86_002138"
                     /EC_number="3.5.4.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287154.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA adenosine(34) deaminase TadA"
                     /protein_id="HEG1169665.1"
                     /translation="MVNTNLDAVEKEKWMRLALAEAKKAEMLHEVPIGAVVVLDGKVI
                     GRGYNLRETTQDATTHAEMLAIKEACEKVGSWRLEDAALFVTLEPCPMCSGAMILSRV
                     AEVYFGAYDPKGGTAGTLMNLLEDERFNHQAYVEGGILEDECGMILTDFFRKLRQRKK
                     KLKKDWSTNEK"
     gene            8843..9700
                     /locus_tag="SCJ86_002139"
     CDS             8843..9700
                     /locus_tag="SCJ86_002139"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353248.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HEG1169666.1"
                     /translation="MKMDKLLALYPQAQKKNSPASEADILSFAIDDAFIWIKQDSLSQ
                     QETSLLKALFPVINDQKKHPWYRYLFKDASCVQEKSFRLIQLHVESKGEFLKKEWQDT
                     ILELFRYSADFFFYTDTDALLVEQINRDYLDATEINGIFLSLDADFDTTTSVFVGSFH
                     SPGTDLVPLFAEERKIFLTEQNNLYTHSRTFSMQDVALHYYTKDTVKDSKLIKSYIEL
                     IKQNEMEVIIQELWNELGNVSSAAKTLFLHRNTLKYRIEKFQEQSGFNLKKANDLLFC
                     YLLLLHEHN"
     gene            complement(9781..10116)
                     /locus_tag="SCJ86_002140"
     CDS             complement(9781..10116)
                     /locus_tag="SCJ86_002140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEG1169667.1"
                     /translation="MKTSTKVTIGLSVAAAASVATAVVVSGKVMEKIHHMTTRTKVKK
                     FVYDKFDGNEKLLEIVDGLSDSDLDSLMGMLAKIKSGKKKISVYGDSIKDSTEDVKDR
                     LMHFVEKMM"
     gene            complement(10183..11553)
                     /locus_tag="SCJ86_002141"
     CDS             complement(10183..11553)
                     /locus_tag="SCJ86_002141"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334069.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RsmB/NOP family class I SAM-dependent RNA
                     methyltransferase"
                     /protein_id="HEG1169668.1"
                     /translation="MKEEATTLPQQFIKKYRLLLGEEASDFFSALEQGSVKKGFRWNP
                     LKPAGLDMVQTYHSEELQPAPYSNEGFLGTVNGKSFLHQAGYEYSQEPSAMIVGTAAA
                     AKPGEKVLDLCAAPGGKSTQLAAQMKGKGLLVTNEIFPKRAKILSENIERWGVSNAIV
                     TNHAPAELVPHFSGFFDRIVVDAPCSGEGMFRKDPNAIKEWTEESPLYCQKRQQEILS
                     SAIKMLKNKGQLIYSTCTFAPEENEEIISWLVENYPVTIEEIPLTQSVSSGRSEWGSV
                     AGLEKTIRIWPHKDQGEGHFVAKLTFHGQNQMHKEKKTRKKSKVQMTKEQEKLWTEFS
                     NDFHYEATGRLLVFNDHLWEVPELAPSLDGLKVVRTGLHLGDFKKNRFEPSYALALAT
                     KKIENIPCLPITQKEWQSYTAGETFQRDGNQGWVLLVLDKIPVGFGKQVKGTVKNFFP
                     KGLRFH"
     gene            complement(11631..12167)
                     /locus_tag="SCJ86_002142"
     CDS             complement(11631..12167)
                     /locus_tag="SCJ86_002142"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300636.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HEG1169669.1"
                     /translation="MMGRVTNYSKEYLLFKSMVYVEEYGMKSITARELAEFCGCSTYP
                     IYTHFKSISGLKEKILEEITVYFENYLAECSSDDVLSIVYLLKDFFSIHESIREIIAK
                     SDLDMDSLFKRPFFSYVKGHTKIKEEKSLELVWLNALGSLRSDDSSQDFLEMMNAVWD
                     KNEVTDEYLENILKEQSP"
     gene            12476..13957
                     /locus_tag="SCJ86_002143"
     CDS             12476..13957
                     /locus_tag="SCJ86_002143"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298955.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HEG1169670.1"
                     /translation="MLIEARKKELLVLQMVIQKNNCSLQELSNDLHIPKRTIKELIRK
                     INVSLKQFLLIDEFIYSNHKGEIQTKETDQTKKLAVFSKLKLCYLKESNRFNYLMLLI
                     NYPQTAVPKKYLLEQLYISPSYLEKLTKQLNHSLKKFHLRILSVKNCCTLEGNELSIR
                     LYLYFFLSDTFGGNDWPFSSLELNALKMNSTDEQIEKISKQEKNIYLLLTLFILRNKQ
                     QAYFPKTSSEITELMDLLQHTHDYSEYFTSFSVSRPCEETETERAYFNYFFYCFFPNQ
                     ISFRQKQEIGFLFSTSKNLICRQVGQLMTYLTQQFPLNHLENKIPIYFYFLVLAVVFF
                     ELLGESSSSFSTLCFPEATSNHSSSFIQEKKLREKIDTIFSKTGERNQISQYLSQLIQ
                     HLLQTETKEQLLIHLQITKQFTGNYHLQSHLQQLFRKDAVKITAYSEHADLIITDQHE
                     KISDETETFYLDTTHNTVLFGKLIKAVHELYMKKGYENKTKRM"
BASE COUNT         4296 a         2796 c         2298 g         4593 t
ORIGIN      
        1 gaaattaagt atattcgcta aaccagtctt tccttcagaa agttccgcta acgcaaattc
       61 caacttgtca tcaattgaat ggaccaaaaa ttttttagta ccatcctcca aatgcaacat
      121 aattgtacca gcaactcttt tgcttgccaa aatttccacc tcgatcaact tgttctatgg
      181 cttacataaa aattctagca cattttcaga aaaaatacac tttttatttc ttattttctt
      241 tccgttctag ttcagataat gcttcttcta tccaaatagg taactgctcc gcaatagtgg
      301 taaatccgat cttacgattt ttattcgtga aatacctttt tctgcgatat ggaacagctg
      361 gatttgtttt ttctaacgtt cttaaagata aactgatttt tttggaatat tcatcaatgt
      421 cgatgatctg aactgttact ggctgtccga cttttaacaa tgaatggata tttttcgtat
      481 aaccggattg tacttctgaa acatgaataa gtccttgcgt ctcgttatca agagaaacga
      541 atgcgccata aggctgtatc cctgtcactt ttccttcaat tatttgtcca attttataag
      601 tcatctactt ccctctcttc gcatttatca ttatactgta aatagctcaa aaaaagaagc
      661 gacgaaaatg actggaggtc ttaagatgat cgaatttgat aaacagattt caagaaaaga
      721 tacagaaagt gtaaaatggg acgcaattgc tgagacatat caaatggatg acttacttcc
      781 tttgtgggta gcagatatgg acttcttgtc tcccggtggg gtagctaaag cgtttagtga
      841 atacatcaaa catggaatct ttggttatgc cactctgtct gataaactat atcaggctat
      901 cattcattgg gaacgccaac accatgctgt ggagttgacg aaagaaaata tcgtttttac
      961 aagtggtgtg ttaaccagtt tagctgcagc agtacaaact tttacaaaac caggcgattc
     1021 tattttgatt catgacccag tttatcctcc attttcctct attatagaaa caaatcaacg
     1081 tcacatcgtt cgaagctctt tgctagaaaa aaataatcat ttcatcatgg acttgtctga
     1141 catggagaaa aaaatcgaag aaaacaatgt aaaagcaatg attttatgca atcctcacaa
     1201 tcctggaggg cgtgtttgga gcaaagaaga attgaaacag ttaagcgaat tgtgtttaaa
     1261 acatcaagtg attctgttta gtgacgagat ccatcaagat ttaactttat tcgatcatac
     1321 atttacttcc atgctaacga ttgatcctaa tttagatgat ctactagttg cttttacttc
     1381 cgcaaccaag actttcaatc tagctgcaat caaaaattca atggtattta tcaaaaatcc
     1441 agagatgaaa gctgattttg aaaaacattt agtgatgaac caacagcacg aaatcaacac
     1501 ttttggactg attggaaccc aagcggctta tgagacgggt ggagaatggt taagccaact
     1561 gattccttat ctagaaaaaa atgttcaaac agttaatcag ttttttgaac agtatcttcc
     1621 gaaagtgaga ataatgaacc ctgaaggaac ttacttaatg tggttagatt ttaccgccta
     1681 tatttcagac gaccaagaat tggagaacac tcttgttcgg aaaggaaaag tcgtattaaa
     1741 tcctggcatc acgtttggtc cttccggtca tgggcatatg cgcttgaata ttgcttgtcc
     1801 agaagaaact ttactggaag gacttaatcg aatcaagtat gctttagaga caatcgatta
     1861 aatcatttat tcaaaataaa aggacgagac cttactaggt ttcgtccttt ttcataatta
     1921 ctccgtacca taccaaacta ttctgaaatt tcaattgttt caatcactac atcatgtaca
     1981 ggtcggtctt gtggaccacg ttgtacattt gcgatttcat ctactgtgtc catcccttct
     2041 agtacatgtc caaagactgt atgtcggaaa tctagccaag gagttccgcc acctttatac
     2101 gcttcaatga tttctgatgg gaatccagca ccttctagtt gtccaagcat attagctggc
     2161 acatttgtgt tatttacaat aaagaattgg cttccgtttg tgtttggacc tgaatttgcc
     2221 atagataaag ctccgcgaag gttgaacagt tcacgactga attcatcttc aaaactttcg
     2281 ccataaatac tttcgccacc cattcctgtt ccagttggat cgccgccttg gatcatgaaa
     2341 tcaggaataa cgcggtgaaa aatcacgcca tcataataac cttttttgga aagttcaatg
     2401 aaattttgaa ccgtcttcgg tgctagttca gggaataatt gaacagtaat gtcaccacgg
     2461 tttgttttga taacagcttt tggtcctttt tcacttttta aatctagttg tgggaatgcc
     2521 atattgtttc ctcctaatat ttgcatactt cataataata ataacataat acctaacctc
     2581 tcgtaaaaat actagatttg ttttctttta tttttcatca ctagatacat tctataactt
     2641 accgtttcga tttcgaattc aaacattttt gagaagcatt ctcaattaaa ttctgctata
     2701 gtgtgaaaag ggaggaattt ttatggaaat ctatgacatt acaatcatcg gtgcaggtcc
     2761 tgtagggatg tttgccgctt tttacgcagg aatgcgccaa gctaaaacaa aaatcattga
     2821 cagtctacct caacttggtg gtcaattagc aactttgtat cccgaaaaat atatttacga
     2881 tattcctggg tatccagcaa taaaagcaag tgaattaatc gatcaattag aaaaacaatt
     2941 aactactttt aaccatacat tccatttaaa agaagaagtt ctctcgctta ctcgtgaaga
     3001 cgaagttatc gagattacta caaataaagg catccattat tccaaagcag tcattttagc
     3061 tttaggaaat ggttcattcc agccaagaaa attgaattta gataatgctg aatcatttga
     3121 aaaccacgga ttggattatt tcgtgaatga tttgatgaag tatgctggaa aaaaagtcgc
     3181 tattgctgga ggtggtgatt ctgctatcga ttggacgttg atgttagagc ctattgcaag
     3241 cgaagtttac ttgattcatc gtcgcccgga atttcgtgcg cacgaacata gtgtatcacg
     3301 cttgaaatct tcatctgtca acttgcttac tccctatctc atcgatggat tatcaggcaa
     3361 taatggggaa ctgacagata tccggttaaa aaaagtgaaa agtgacgaga cgattgattt
     3421 gatgattgat tctctgatcg tcaattatgg attctcttct aatttagaac atctttcttc
     3481 ttggggactt gatagtacca gaaatgccat tactgtaaaa tcagatatgt ctacttctat
     3541 tccaggcgtt tatgcagctg gtgacatctg tacctatgaa ggaaaagtga aactgattgc
     3601 tactgggctc ggtgaagcac ctactgcagt gaacaacgct ttacactata ttaatccaaa
     3661 ggaacgaact cagcctggtc acagcacaag cttatacgat aaaaatcttc gtcctagttg
     3721 attttttata tctctttgca tttcaagtta aacccgaaag cagtttcttg catttttttc
     3781 atgttccgtt aacatgaaat agagaaagaa aaaataaagg gggagcaata atatggtcgt
     3841 caaaactgag actttagaag aaaaggcaag agaactattg aaggaacgtg gcgtaactgt
     3901 agaagatatc gcacaacttg tcatgttttt acaaaaagac tatatttctg atttgacttt
     3961 ggcggattgt actgaaagtg tcaacagcgt gctaacaaag cgtgaagtac ataacgccat
     4021 tatcactgga atccaattag atatattagc agaaaagaaa aaattgatga gtccgttgca
     4081 acatattatc gaagatgacg aaggccttta cggtatcgat gagatcctag ccttgtcaat
     4141 cgtgaatgtt tacggtacga tcggctttac gaattatggt tatatcgaca aggtgaaacc
     4201 tggtatttta aaagaactaa acagccacga tggcgttcat gtccatacct tcttagatga
     4261 tatcgtcgga gcgatcgccg ctgctgctgc cagtcgatta gcacacaaag aaccagaaaa
     4321 aagaagtcgc cttactcaat aattcaacaa aaaaagaaag aaaacgtcta tttagacgag
     4381 ttctttcttt tttgtttgtt tcaagctatt ttttcctaag aaaagaaaaa cagcaaaaaa
     4441 taattctaat aatacaaaaa ataagataaa gctatgcatg aaaaattctt ctggaagtac
     4501 attgatgatt ggatcagtaa cgggatcgaa taaccagtca tcgttgttga aaaacgtttc
     4561 atgaaaaagg ataaaaaagc gatcaaatcc tatcatcatg aaaaaaccga aaacaactgg
     4621 cagtaacatg ccgatttgaa acggacgaat caaccgccaa agccgatcat tcttttgcaa
     4681 atatttgata aacaaaatac tcgggatcaa agtgatgaaa aacacagcat aatccactaa
     4741 aaacaaaatt ttcacttcat aaaagtgatg ggctccgcta gcagaaactg ggaaatctgg
     4801 caagctgagg atggttttga agggattatt gagataattc attaggtgat caaaattttt
     4861 cagtaacgtc tcttgatcta aagaagtata atccaaaatt tgcaaatgtc cgatatcaaa
     4921 tacgtatagt ggtcgaaagt tgatcgtcac accaactgcc agactgatca gtgtcagaaa
     4981 taggctgaac aatccagcat attccatcca ctgccatctc gaatttttca tattagaagc
     5041 tccattcatc cagtgattgt ttcacatagg tagctgggat aggcaaccca ggtacatctt
     5101 cttcttttgt aaaacctgat aaaacgagca aagtatctat attattgcgt atcccagctt
     5161 ggatatctgt ttcatagtta tctccaacca tgattacttc ttctttttgc agccctaata
     5221 cttcaacggc tttatccata atgatggctt ctggttttcc gatgtagatc ggcggcgttt
     5281 gggttgctgt ctctacaaaa gagatgactg accctgctcc aggaagaagg ccgcgttcag
     5341 taggtatatt cttatctggg ttcgttccaa tgaaagtagc acctttttga atagcaagtg
     5401 ttgctttaac tactttttca taggtcagat aattatctaa gcctaccact acatagtcag
     5461 gagcctcttc ttcccatgta aatccggcag ctaaaatcaa gtcaatcaat cctgcttctc
     5521 cgataacata gacttttttc ccttttccat ctgctttcat aaaatcgatt gttgccaaag
     5581 tagctgtgta gactgtctct ggagctacat gaatgtcaaa ctcatctgct agacgattag
     5641 ctactgtttc tggtgactta gtcgtgttat tggtaacaaa caagaaaggt aattttcttt
     5701 tttgcaattc ttccacaaat cgtttaccgg ccggtatggg ttgtgtccca cgataaatcg
     5761 ttccgtctaa atcaattaaa tatcctttat agtccattca tccactactc ttcccttttt
     5821 ctgatcgtaa atgctgtttt tgttttttct acttgtttag gttgttcttg tttttttact
     5881 ttttcagagg tttttgctga ctctgttttt cgatttttaa taaccggttt tttgcgatta
     5941 gatgcgactg gtcctttttt ctcatcgata tacgcacgat tatggttttt tttctttttt
     6001 cggcgctgag ttgtcttttc ttttttaccg ccgattctct cgatgacaaa atacgcacaa
     6061 ccaaaattgc aatattcgta aagatagtct tccaaggaat cgattcgctg atctggatgc
     6121 gcccgtcgat tatccgcttg gaaaaatcct tttaggcgta gttgttcata accccaatct
     6181 cctacgatat aatcatatcg ggctagtaca tcactgtaac gttctccaag cttttctgca
     6241 ttaaatcctt cacgataatc ggacacaagc cgataatgtc tttcacctat caaaaaatta
     6301 ctgtcatcta acagtgtcac tagctcgcct ttgacttttt ctgctttagg ctcctcaacg
     6361 atttcttcca aagcagctgt cagttcttcg gtgactgttg tttcctgttc agctgatgat
     6421 tttttcttag caatttctgt tttatgctca acgttctgct gttcattttg ttttgcagac
     6481 atgcgttcac cacctatctt ttttattata ccttgttttt atttgattgg gtagtgagct
     6541 ttcaagtaat ctcctaccac acttcggata aattcaggga acaaaaattc attttttcca
     6601 gcaatctcaa tcgttggaaa aaatggaaca aacatcaaat gatctacggt agtaaaagta
     6661 taatttttat tcagatcgat tggcttgttc ttccaaaaga cttgatggtt aatagaatca
     6721 tatgtgattc cattatagac catctgccca aaaatcttac cgcgaaatcc cattcccttc
     6781 ataggatagt tacgtaaaaa gttacgattt ttttctattt ctaaaactaa gcggatcaga
     6841 tcatttccac ccaatgtgac atttaacaag tgcatcggat gcggcaacgc agtgtgaagc
     6901 tgatcctgat tgacccgccc ttttggaatt tcagttaaaa acagaccact gtttaatatt
     6961 gctgcttccg tctttcctcg ttctttcacc gcctcaaagg ctacttggat aaacgaatgc
     7021 tcattgaaga tatctagact cagagaaaat ggcaaatctg ctacttcttt ttcttttaat
     7081 aattcatgcc cacgttctag ataactattt atttcttgtt catcttctgt aaaagctgtc
     7141 attgtttcgg tagggatggc tttggctgaa tgcttaagga tttttttctc ttcatcgatg
     7201 gttaaatgga cttcaccgac atactgcccg tactttccag cggctgctat ttggacatca
     7261 ttgattacgt gaccttctct gaataaatga tgggtgtgcg atcctaaaat aacatcaatt
     7321 gctggtattt cttttgcaat ttggaaatca tcctgaatcc cgagatgact catcaaaact
     7381 aaaacatcca cttgtgaacg taaatcttcg actaattctg gcaaaatatc ataaggattc
     7441 cgtatgtccc agccattcgg attataagta agcggaaatg gagcagtaaa tgcaattagt
     7501 ccgattttcg tctggtgttt cgttgtaatg attttatatg gtttcgtcca tgccggtggc
     7561 tggagcgtat ttttatcgaa taagttatcc aggatgatat caaactcagc atgatcgtat
     7621 aaatgattca actcttcttt ggaatttcct acgccttcat tgtttcctat cgtcgctgca
     7681 tcataaccaa cttcattcat cagcaagata ttttcctgtc cattcgttgc ttctgacaat
     7741 ggatgccaac gatccacaaa atcacctaga tctactgcaa tcgttgtcgt tttttctttc
     7801 ttttcgtttt ctcgtttacg ttgatccaaa aaccttctga tccttggcca attttctaaa
     7861 tgagaatgca gatcattcgt atgtaaaatc actatttttt ccatgtaccc tctcgcctct
     7921 ctttatcttt atcataccat aggcagcttt ttcttccatt gaaacaagct ctatttcaga
     7981 gtttttcctg ccttgaagca aattgtataa gtacaatatt cgataaatca gtctggctaa
     8041 aaaatcagga acattcattt ttacagtact ataaaaataa aaaaaacaga cccgaaagtc
     8101 tgttgtcgtt tcattataat cagacacatg gcggaacctg cttagtgctg cttccttcca
     8161 gacctgacac gcttcacaag cccaccattg tcctagcctt tcggcaaaga atagtatacc
     8221 ttatttttca ttcgttgacc agtctttttt taatttcttt tttcgctggc ggagctttcg
     8281 gaaaaagtca gtcaaaatca tcccacattc gtcttctaaa atgccacctt ctacatatgc
     8341 ttggtggtta aaccgctcgt cctccaaaag attcatcaag gtaccggctg ttccgccttt
     8401 tggatcatac gcgccgaaat aaacttctgc tacacgagac aaaatcatcg cgccgctgca
     8461 cataggacaa ggctctaagg taacaaaaag tgcagcatct tccaagcgcc agcttcccac
     8521 tttttcacag gcttccttga ttgcaagcat ttctgcatgt gtagtagcat cttgcgtggt
     8581 ttctctaagg ttatatcctc ttccaatcac ttttccatcc aatacaacca ctgctccaat
     8641 cggaacttca tgaagcattt ctgctttctt tgcttctgcc aatgcaaggc gcatccattt
     8701 ttctttttct actgcatcaa gatttgtatt taccaattac ccttccctgc ctttgttttt
     8761 tcttgtttta ctttagcata tgataaaaag gctgtaacta actttttttt ctatggtaaa
     8821 ataaatacaa gaggtgaaca agatgaaaat ggataaatta cttgctcttt acccacaagc
     8881 acagaaaaaa aactctccag catcagaagc ggatatcctt tcttttgcaa ttgatgatgc
     8941 ttttatctgg atcaaacaag attcattatc tcaacaagaa accagccttt tgaaagcgtt
     9001 attccctgta atcaacgatc aaaaaaaaca cccatggtat cgctatcttt ttaaagatgc
     9061 ttcttgtgtt caagaaaaaa gctttcgttt gatccagctt cacgtcgaat caaaaggaga
     9121 attcttaaaa aaagaatggc aagatactat tttggaatta tttcgctatt ctgctgattt
     9181 ctttttttat acagacacag atgcgctctt agtcgaacaa ataaaccgtg attatcttga
     9241 tgctactgaa atcaacggta tttttctatc tttagatgct gattttgata cgacaacgtc
     9301 tgtattcgtt ggttctttcc attcacctgg caccgacctc gtccctttgt ttgctgaaga
     9361 aagaaaaatt tttttaacag agcaaaacaa tctttatact cacagccgta ctttttctat
     9421 gcaagatgtg gctttacatt actatacaaa agatactgta aaagatagta agttgatcaa
     9481 aagttacata gaactgatca aacaaaatga aatggaagtg atcattcaag aattatggaa
     9541 tgaattaggc aatgtcagct ctgctgcaaa aacactgttt cttcatcgta atactctaaa
     9601 atatcgaatc gaaaaatttc aagaacagtc tggtttcaat ctaaaaaaag ccaacgattt
     9661 actgttttgc tatcttttat tgcttcatga acataattaa agatagatta aaggggttgt
     9721 gacaataatt ttgtcacaac cccttatttc gaatgaatag tgttcaaaag ctagtttctc
     9781 ttacatcatt ttttcaacaa aatgcattag ccggtctttt acatcttcgg ttgaatcttt
     9841 gattgaatca ccataaacag agattttctt ttttcctgat ttgatttttg ctaacattcc
     9901 catcaaagaa tccaagtcac tatctgaaag accatctacg atttctagta atttttcatt
     9961 tccatcaaat ttgtcataga cgaatttttt tactttcgtc cgagtagtca tatgatggat
    10021 cttttccatt acttttcccg atacaacgac tgctgtcgct acactggcag cggctgctac
    10081 gcttaatccg attgttactt ttgttgatgt tttcataagt atcagctcct ttttatctat
    10141 ctccatcata gcatagacaa aaaaataaac gaactaatcc actcagtgaa atctcaatcc
    10201 tttagggaaa aagttcttta ctgttccttt tacttgtttc ccaaatccga caggtatctt
    10261 atctaatact agcaaaaccc atccttgatt cccatcacgt tgaaatgttt ctcctgctgt
    10321 gtaagattgc cattcctttt gagtgatcgg caagcaaggt atattctcta tcttttttgt
    10381 agcaagcgct aatgcatagc taggttcaaa tcgattcttt ttgaaatctc ctaaatgcaa
    10441 acctgtacgt acgactttta aaccatcgag agaaggggct aactcaggaa cttcccaaag
    10501 atggtcgttg aacactaaaa gtcttccagt agcttcataa tgaaaatcat ttgaaaactc
    10561 cgtccagagt ttttcctgct cttttgtcat ttgcacttta cttttttttc gtgtcttctt
    10621 ttctttatgc atctgatttt gtccatgaaa agtcagcttt gcaacaaaat gtccttcacc
    10681 ctgatcttta tgaggccata tacgtattgt tttttccaaa ccagctacgc ttccccattc
    10741 agaacgtcct gaactaacgg attgagtcaa aggaatctcc tcaatcgtta ctggataatt
    10801 ttctacaagc cagctaatga tctcttcatt ttcttctggt gcaaacgtac aagtcgaata
    10861 aatcagttgt cctttatttt ttagcatttt tattgctgaa gacaaaatct cttgctgacg
    10921 tttttgacag tatagcggag attcttctgt ccactctttt attgcatttg gatcttttcg
    10981 gaacattcct tctcctgaac aaggagcatc aaccacgatc cgatcaaaaa agcccgaaaa
    11041 atgagggaca agttctgcgg gtgcatgatt tgtcactatt gcgttcgaaa ctccccatcg
    11101 ttctatattt tctgataaaa tcttcgcacg tttcggaaag atctcattcg tcactaataa
    11161 ccctttcccc ttcatttgag cagctaattg ggttgattta ccaccgggag ccgcacacaa
    11221 atccaaaact ttttcaccgg gttttgctgc agcagctgtt ccaacgatca ttgcacttgg
    11281 ttcttggcta tattcatacc cagcttgatg gaggaaagac tttccattga cagtgccaag
    11341 aaatccctca ttgctgtagg gagcgggttg taattcttcg ctatggtaag tttggaccat
    11401 gtccaatcct gccggtttta gtggattcca ccgaaatcct tttttcacgc ttccttgttc
    11461 aagcgcagaa aaaaaatcgg atgcttcttc gcctaaaaga agacgatatt ttttgataaa
    11521 ttgctgtggt aacgttgtcg cttcctcctt cattccaatg tttcgttaga cattttacaa
    11581 tcttttttac gaaaaataaa ggtggctgat ctactttttc atgttgctgc ctaaggtgac
    11641 tgttccttta agatattttc caagtattca tccgtcacct cgtttttatc ccaaacagcg
    11701 ttcatcattt caagaaaatc ttgagaagaa tcatctgagc gcaaacttcc caatgcatta
    11761 agccatacca attcaagcga cttttcttct ttgatcttcg tgtgaccttt gacatatgag
    11821 aaaaatggcc gcttaaatag cgaatccatg tctaaatcag attttgcaat gatttcacga
    11881 atactttcat ggatagaaaa aaaatctttc aataagtaga caatgcttaa aacatcatcg
    11941 gaactgcatt cagctaagta gttttcaaaa taaacagtga tttcttctaa tatcttctct
    12001 tttaacccgc tgattgattt gaaatgtgta taaatcgggt atgtagaaca accgcagaac
    12061 tctgctaact ctcttgcagt aattgacttc attccatatt cttccacgta taccatactt
    12121 ttgaacagca aatattcttt actataattt gttactctcc ccatcattct tacctccgtc
    12181 tcttttcatc tacccataaa taaataacat aaataattct aaaaatatta ttgataatta
    12241 tcaaaacata tttagttata tccattaaaa atcaattttt ccgccttaaa aacaagaata
    12301 tcttgacaaa ataccctagg aaataaacaa tagaataaac aaggtaacaa aaaaattaaa
    12361 taacgttata taaaaaagaa ggcgacactc tgtgagaata acacagactg aaatgacttt
    12421 ccactcacag tttttgtcta actatataca aaaacagaat aaaaaggagg tatttatgct
    12481 aattgaagca agaaaaaaag agttacttgt tctacaaatg gtcattcaaa aaaataattg
    12541 ttctttacaa gaattatcca atgacttaca tattccaaaa cggacaatca aagaactgat
    12601 ccgaaaaata aatgtatcac tcaagcaatt tttattgatt gatgaattta tttattctaa
    12661 tcataaagga gagatccaaa ccaaagaaac cgatcaaact aaaaagttag ctgttttttc
    12721 taagttaaaa ttatgctacc taaaagaatc caatcgtttt aactatttga tgttactaat
    12781 caattatcca caaactgctg taccaaaaaa atatttactt gaacaacttt atatatcgcc
    12841 ctcctatctc gaaaaactga caaaacagct caatcattcc ttaaaaaaat tccatttacg
    12901 gatcctatct gtaaaaaatt gctgtactct tgaaggaaat gagttatcta tccgtctcta
    12961 tctttacttt tttctttcag atacatttgg aggtaacgat tggccatttt cctcacttga
    13021 attaaatgca ctaaaaatga acagtacaga tgagcaaata gaaaaaattt caaaacaaga
    13081 aaaaaatatc tacttgctgt taactttatt catattacga aacaaacaac aggcatattt
    13141 tccaaaaact tcttctgaaa tcactgaatt gatggactta ttgcagcata cacatgatta
    13201 ttcagaatac tttacttctt tttcagtgag tcgcccttgt gaagagacag aaacagagag
    13261 agcctacttt aattattttt tctattgttt ttttccaaat caaatctctt ttcgacaaaa
    13321 acaggagatt ggttttttat tttccacaag caaaaacctt atttgtcgac aagtcggtca
    13381 attgatgact tacttaactc aacagtttcc tctcaaccat ttagaaaata aaataccaat
    13441 ttatttttat tttcttgtgt tggcagttgt ttttttcgaa ctgttgggtg aatccagcag
    13501 tagtttttct acactttgct ttccagaagc cacgtccaat cattctagtt cttttataca
    13561 ggaaaagaaa ttaagagaaa aaatcgatac aattttttca aaaactgggg aaagaaacca
    13621 aatcagtcaa taccttagtc aattgatcca acacttacta caaacagaaa cgaaagagca
    13681 gttactcatc catttgcaga taaccaaaca gtttacagga aattatcatc ttcaaagcca
    13741 tttgcagcag ttatttcgca aagatgctgt taaaattacc gcatattcag aacatgctga
    13801 tttgatcatt acagatcagc atgagaaaat ttcagatgaa acggagactt tctatctgga
    13861 tacaacgcac aatacagtgt tatttggcaa gttaataaaa gcggttcatg aactgtatat
    13921 gaaaaaaggt tatgagaaca aaacaaagag gatgtgacaa aattcttgtc acatcctctt
    13981 att
//
LOCUS       DARBET010000064        13908 bp    DNA     linear   BCT 07-NOV-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4027
            SAMEA114569841-rid20112183.denovo.066, whole genome shotgun
            sequence.
ACCESSION   DARBET010000064 DARBET010000000
VERSION     DARBET010000064.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA114569841
            Sequence Read Archive: ERR12203666
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 13908)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13908)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001972618.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001972618.1
            Genome Coverage       :: 132x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/07/2023 13:18:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,041
            CDSs (total)                      :: 2,975
            Genes (coding)                    :: 2,908
            CDSs (with protein)               :: 2,908
            Genes (RNA)                       :: 66
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 67
            CDSs (without protein)            :: 67
            Pseudo Genes (ambiguous residues) :: 0 of 67
            Pseudo Genes (frameshifted)       :: 26 of 67
            Pseudo Genes (incomplete)         :: 28 of 67
            Pseudo Genes (internal stop)      :: 20 of 67
            Pseudo Genes (multiple problems)  :: 6 of 67
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13908
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA114569841-rid20112183.denovo.066"
                     /isolate="VRE4027"
                     /db_xref="taxon:1352"
                     /country="Denmark"
     gene            206..544
                     /locus_tag="SCJ86_002144"
     CDS             206..544
                     /locus_tag="SCJ86_002144"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEG1169671.1"
                     /translation="MPDYKQKAGYSFCSDKFQLGKFTKLAGEEVSTVRIKECPLQIET
                     LVQRISTRQDFAIVEREIVAIFVEEGILHDPTHINVDKWQPLIYKFREYTTANQSLTL
                     NFRFQEFKES"
     gene            complement(749..1324)
                     /locus_tag="SCJ86_002145"
     CDS             complement(749..1324)
                     /locus_tag="SCJ86_002145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349929.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SOS response-associated peptidase family
                     protein"
                     /protein_id="HEG1169672.1"
                     /translation="MCGRYFFDLESELLQNYYREAQTKQPKQAAELAAGEVFPSNLVV
                     TLRKEEENILTPEIMKWGFTGFKKGQLMINARAETVEEKKTFHQPFLQNRCVFPMSGF
                     YEWNEEKDKFMFSNKEKIVYIGGFYRSHQDEKMAESIIMTTEPNRTVAPIHDRMPLVL
                     TEEQIEPWVTDISFARKIITQQMPELVMEKV"
     gene            complement(2056..2637)
                     /locus_tag="SCJ86_002146"
     CDS             complement(2056..2637)
                     /locus_tag="SCJ86_002146"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374932.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HEG1169673.1"
                     /translation="MKTKEKILEATKDLIYKKGYHETSIRDILAASDTGKGQFYYYFS
                     SKKEVCLAVIHAHMDVWQTELFEDILNAGKQPEEALEEMLDWIFADHETQITYHGCPV
                     GNLIIELSTVDEEYRLLLLELVNQWVKYTKQNILALSEEKLSDEEADRQAKALIAEIQ
                     GSILLLKVTQDLSLLRQNFQLIKERLLTSQKVL"
     gene            complement(2820..3446)
                     /locus_tag="SCJ86_002147"
     CDS             complement(2820..3446)
                     /locus_tag="SCJ86_002147"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334962.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine hydrolase"
                     /protein_id="HEG1169674.1"
                     /translation="MFNNKNTAVVLTDPQVEFLKPAGGGFGLTKDILEKYNTIENMIE
                     MVKQAKEKGYKLFISPHYFYPHDHKWQFGGAGEHMMLEQQMFARKSQYESVEEGSGAD
                     FIEELKPYLDENTIISSPHKIFGPESNDLALQLRKNGIDTVILGGMNGNLCSDSHMRE
                     LVESGFNVVVATDAIGAPGQEAMDAALTNFGFIADQVMTTEEVLEQLS"
     gene            complement(3468..4799)
                     /locus_tag="SCJ86_002148"
     CDS             complement(3468..4799)
                     /locus_tag="SCJ86_002148"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631699.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-containing oxidoreductase"
                     /protein_id="HEG1169675.1"
                     /translation="MMEKYQNIVIGFGKGGKTLAKALASKGESVLVVEKSTRMYGGTC
                     INIGCIPSKSLIFNGERGIDFTEAVARKEKLTGMLRAKNYHMISDEATGTVMDGTARF
                     LSNHQIEVTNNGEKVIVEGERIFINTGSEPIILPIKGLNTSRYLIDSTQAMDQEQLPE
                     KLVIIGAGYIGLEFASMFNEYGSKVVVLDAHSEFLPREDEDIAQMILEDMTNAGIEFH
                     LGVSVDQVADQESTAAVTFTEDGQEVTIQASKVLAATGRKPSTASLGLENTDVKLTDR
                     GAIAVDEYLRTDADNIWAIGDVKGGLQFTYISLDDYRIIMDQLKGENKRTTNNRKAVP
                     YSVFITPTLSNVGLTEKQAKAQGVNYKLFKYMTSGVPKAQVLEDPKGVFKVLVDPETD
                     LILGASIYAEDSHEVINLISLAMNGKLPYTLLRDQIYSHPTMSEALNDVLK"
     gene            complement(4954..5355)
                     /locus_tag="SCJ86_002149"
     CDS             complement(4954..5355)
                     /locus_tag="SCJ86_002149"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343655.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="organic hydroperoxide resistance protein"
                     /protein_id="HEG1169676.1"
                     /translation="MKKAYETAMINHGGRNGEVEAPNGSMHMKITPPGIHAEGTNPEQ
                     LFAAGYASCFNGAVQHMIKEAGMTAESTVKARVSLYNLDDGGYQIGVVLEVHIDGISE
                     EEAQKIAEDAHEFCPYSKATRGNIDVEVTIV"
     gene            complement(5441..5647)
                     /locus_tag="SCJ86_002150"
     CDS             complement(5441..5647)
                     /locus_tag="SCJ86_002150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002308156.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEG1169677.1"
                     /translation="MVTNMVIFLTVLVYGVNFLVYMYLKINHIKDYVEKIAIYFGTNM
                     TLLFTSSIFLFFGKIVEDGILVFE"
     gene            complement(5742..7244)
                     /locus_tag="SCJ86_002151"
     CDS             complement(5742..7244)
                     /locus_tag="SCJ86_002151"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293678.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphomannomutase/phosphoglucomutase"
                     /protein_id="HEG1169678.1"
                     /translation="MKDMKALQNGSDIRGIALPVEGYEVNLTTDMVKKIGWGLANWIK
                     KKKKSTDPNKKWTIGIGHDSRISGPALKEALIQSLKEQGIHVIDFELATTPAMFMSTQ
                     FEQFSCDAAVMLTASHLPYYYNGIKFFTSEGGAEKEDIAFILSQQQKIKSEQTGSVEK
                     ADLLTPYAQDLVTKIKKGIGTLESKPLNGWKIIVDAGNGAGGFFAEKVLNSLGADSTG
                     SQFLEPDGRFPNHIPNPDNKEAMESIKQAVLANEADLGIIFDTDVDRSAVVSASGTVI
                     NRNNLIAVLSTIVLKEHPGTRIVTNSPTSSHLQRFIEEKGGIQVRYISGYRNVINKAL
                     ELNRLGKDTQLAIETSGHAAFKENYFLDDGAYVIAKILMLLPELKRKGITLDEQIKEL
                     KQPVEVQEIRLKLFSDEPREQGNKILEEMKRLLEEEKGMNFDPENEEGIRYDLEEPYG
                     NGWFLIRLSLHEPLLVLQIENDEFGKNKLALVKLAKLFGHFSDLESLTAE"
     gene            complement(7262..7717)
                     /gene="argR"
                     /locus_tag="SCJ86_002152"
     CDS             complement(7262..7717)
                     /gene="argR"
                     /locus_tag="SCJ86_002152"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853090.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginine repressor"
                     /protein_id="HEG1169679.1"
                     /translation="MKKKQRQALIRQIITEQPIGTQEELLARLHEAGADVTQATISRD
                     IREMKLVKSQNENKIVRYTLFNQPSVSLNEERLRTAIRREVLRIQSVQFMVIVLTERN
                     GADVVTNWLDEVAYPEVVGTMAGVDTFIIICRSEEEAQRFAEKLEKMRE"
     gene            complement(7815..8432)
                     /locus_tag="SCJ86_002153"
     CDS             complement(7815..8432)
                     /locus_tag="SCJ86_002153"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GyrI-like domain-containing protein"
                     /protein_id="HEG1169680.1"
                     /translation="MEKIEWRKDKSYSMKKSPQILEVPKKPFFSIRGIGDPNQEDFKR
                     RVACLYAVSYAIRMSPKKNWLIPDYQMYTVYPLEGQWGLQEKYLHEPVMKKEHFSYQL
                     MIRQPDFVTEAIAQEAIQRSSKLPEDLKEQLVFKKMEEGLCAQILHVGPYAEEPATFE
                     KLEAFIAKEGYERASKEHKEIYISDPRKSAPEKMKTILRVKIEKG"
     gene            8653..10026
                     /locus_tag="SCJ86_002154"
     CDS             8653..10026
                     /locus_tag="SCJ86_002154"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293681.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEG1169681.1"
                     /translation="MTKQKQLLLVPAVIATGMMSFAGVLIETAMNVTFPTLIRQFDLT
                     TAQVQWVTTIYLLMISIIVPLSTYLNRNFSLKKLFLASNLLFLAGIMIDFFSPTFMVL
                     LFGRLLQGAATGIALPLMFHIILTFAPLNRRGAMMGVGTLTTAIAPAIGPTYGGIMTS
                     HFTWNHIFLFLVPVLILSLIIGWFAIPEMAVQKSGYLDWISVLGICLLFSGSLTFLST
                     LGTVSSWIALIIGGIGWFLFYYRSKKVEHPLIRLSILSNKTFRLFLIGFLVYQFLLLG
                     VSFVLPNFLQIVQGSSPFVAGLAMLPGAAVGALLSPFSGKMLDQYGPKKPIFAGLILS
                     LIGWLALFLLIGHASVGVLIACHVFYMIGIGLSYSNMMTTGMNALNEELQGDGNAMFN
                     TLQQFSGAVATSLVAVIINYVQHHTSHNYEVSTTLGSKAALGVLLLLLLVSFARFAYY
                     LFFAKKA"
     gene            10101..11213
                     /locus_tag="SCJ86_002155"
     CDS             10101..11213
                     /locus_tag="SCJ86_002155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374911.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-binding oxidoreductase"
                     /protein_id="HEG1169682.1"
                     /translation="MEKIAIIGGGIIGMTLASYLDTSKFNVALFDEGTGQATKASAGI
                     ISPWLSKRRNKQWYRLAKDGAAFFPKLIKDFQLDQTIYAQSGTLLLRPEKDLAELAVL
                     AEKRKEEAPEIGNIEWLSPEQTSQALPLLKPAVSLRISGGGRLDGKAYLEHLGQRLAQ
                     KQMEQIQAKVTLHKKSDSWLIKGPFGEYTADRVILTPGPALKPLLQELGYAADIRPQK
                     GQLLAFQTSYKMSQDWPVAMLDGEADFIPFMDGKILLGATHENDQGWDLAPTSEAYKG
                     LTKSTEQFLTDPDQIKNWPYEFRVGTRAYTSDFSPFFGVLPDDPSLLAASGLGSSGLT
                     TGPYIGYLLANFLNTGHWSTEDYQKDISTYITKNPS"
     gene            complement(11285..12922)
                     /locus_tag="SCJ86_002156"
     CDS             complement(11285..12922)
                     /locus_tag="SCJ86_002156"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286835.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate carboxykinase (ATP)"
                     /protein_id="HEG1169683.1"
                     /translation="MSTKESLELNEITSGNPLLSSIRSIVETTFYGNNVHEVFDRKTA
                     YQLAKGSPGTIITDLTISHAEELDLPADVRTLVFNDGSIVGRTASARRIFEDLDKEQS
                     KYEKILREAVYQSRKRQFYHTKVIVGLSEEFSVQSHLLIPEGFENNLYSYLLNFQPAN
                     SKILETYQKSVSFDEGDLYIFCDPEWTHETFPNGLILFDALHNTAAVLGLRYFGELKK
                     ATLTLAWALGNRNGFIACHGGMKQYASNDQTYTMAVFGLSGSGKSTITLAKHTEGHSV
                     SILHDDAFVISRKNGFATALEPSYFDKTQDYPSDDPAISSFLTCQNVGVTCTSKGEKV
                     LVTGDIRNGNGRTVKSRYATPDRVDHLSEKIDAVFWIMKDDSLPPIIKINDPVLAASF
                     GATLATKRSDAENITTKRQLDELVIEPFANPFRVYPLREDYQAFRELFLKQQTACYVL
                     NTGYFDDKKIKPETTLSIVERIVQQTAEFKSFGPFEDIHYLPIPDFPVSFEDTSYKNL
                     VKQRLDIRLRFIEAQIGKDLLPDEAKESLENLIQKLN"
     gene            complement(13090..13698)
                     /locus_tag="SCJ86_002157"
     CDS             complement(13090..13698)
                     /locus_tag="SCJ86_002157"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002308148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysM peptidoglycan-binding domain-containing
                     protein"
                     /protein_id="HEG1169684.1"
                     /translation="MNSFKKIVLGTTFAAGATAMFVGTTNAHADEVYTVKSGDSLSKI
                     SQKFAGDNSMIDAIAEKNSIANINRIYVGEQLTIPTSNDSSATTENKTTENTASTTET
                     ATQEHTYVAPVETVEVAPAAPAAATAPTSSSAKEWIAQKESGGSYTATNGRYIGRYQL
                     DASYLNGDYSAANQERVAEQYVTSRYGSWDAAKTFWLANGWY"
BASE COUNT         3773 a         2907 c         2480 g         4748 t
ORIGIN      
        1 cctttggaag tatttttgag ttacgtaagt attgatgatc tgtctaactt aatttgacaa
       61 ttaagaaact aaaaaaataa tccaagaaac attgaaccta tggatccaaa agctacccaa
      121 atactatagc taaccccccg ctaccttcaa ttttgcagat aaaacgcttt gggagaaaat
      181 ttcaaagaca acaggccatc ctatcatgcc cgattataaa caaaaagctg gctattcctt
      241 ttgctctgac aaatttcaat tgggaaagtt cacgaaacta gcaggtgaag aagtttcaac
      301 tgttcggatc aaagaatgcc cgcttcaaat agaaacattg gttcaacgaa tttcaactag
      361 acaagacttt gctattgtag aacgtgagat cgtagctatt tttgtagaag aagggatttt
      421 acatgatcca actcatatca atgtggataa atggcaacct cttatctata aattcagaga
      481 atatacaact gccaaccagt cgttaacttt gaattttaga ttccaagaat tcaaagagtc
      541 ataatgataa acatcgttag gtataaataa aaaagactcg cagcacgagt aaaaaacaaa
      601 tagaagctat acattagaaa ggtcgactgt gaccctatag atgcagtata tgcagcttat
      661 gagcatttct tctgttctct ttgtttttgg gatctgtgct gaagtctttt tcgttttttg
      721 ccgctctttc tttgaaaaat cgattatgtt ataccttctc cattactaat tcaggcatct
      781 gctgagtaat gatttttcgc gcaaaagaaa tatctgttac ccaaggttcg atttgttctt
      841 cagtcaagac aagcggcatt cggtcatgga ttggtgcgac tgtgcggtta ggttcagtag
      901 tcattatgat tgattctgcc attttttcat cctgatggct acgataaaaa ccgccaatat
      961 acactatttt ttccttattt gaaaacataa atttgtcttt ttcttcattc cactcataaa
     1021 agccactcat tgggaaaaca caacgatttt gcaaaaatgg ttgatgaaag gtcttttttt
     1081 cttctactgt ctctgcacgg gcattaatca tcaattgtcc ttttttaaat ccggtaaatc
     1141 cccacttcat gatttccgga gtgaggatgt tttcttcctc ttttcttaac gtcacaacaa
     1201 gatttgacgg aaaaacttct ccagctgcta attctgctgc ctgcttcggt tgtttcgtct
     1261 gggcttctcg ataataattc tgaaggagct ctgactctag atcaaaaaaa taacgaccac
     1321 acatcctaat ccctcccaat ctttttttct atcatacact gcaacaagaa gttcgcacca
     1381 acaaaacgct ttcaattttt gtagaactaa aaaagttgtg acaagtgaca aactctccag
     1441 tgaatggtat tctcctgcca actcctcggt cacaagtcac aatattcaac aaacatgaga
     1501 agctgctttt tgaaattgtt ccttgtgact cggagtgaaa cggcaggctt acgacctctg
     1561 ggtaaacggt gttcaaaagc cagtttctcg gtattaagcc aaatttatgc aaaaatgtag
     1621 aaagccattt tcacaaaatt tttcttaata ctcgaaacta agcgactttt tcacaacctc
     1681 ttccttaaac ggtgttcaaa agcttgcatc tgcggtaata agcccaacca aacgaaaaat
     1741 atgagcagct atttttattt ggctgttctt attacttgat gcagggcagc tttttcacaa
     1801 cctctccact aaacggtgtt caatcagcta accttcggta ttagctcaaa aaacggcaaa
     1861 atatgaggaa ctattttgcc gtttgtttct ctaatactca ggtcaaagcg ctgatttcac
     1921 aacctctaat acacggtgtt ccagaagtag cttcttcgaa aaataagccg aaatttcaca
     1981 aaaatttgag aagcaatttt cgtaaattcc ttcttatttc ttgaagctga ccacttctgt
     2041 cacaacctct aattattaaa gaaccttttg cgaagttaac aatctttctt tgatcaattg
     2101 aaaattctgc ctaagtagtg atagatcctg tgtgactttc aacaataaga tacttccctg
     2161 gatttcagca atcaacgctt tggcttgacg gtctgcttct tcatcggaca gtttttcttc
     2221 agaaagtgcc aggatatttt gtttcgtata cttgacccat tgattgacta attctagtag
     2281 caacagacgg tattcttcat ccactgtact caactcaata atcaaattac ctacaggaca
     2341 gccatgataa gtgatctgtg tctcatgatc agcgaaaatc cagtccaaca tttcttctaa
     2401 tgcttcttct ggctgttttc cagcatttaa gatgtcttcg aacaattctg tctgccatac
     2461 gtccatatgc gcatggatca ccgctagaca aacttctttt tttgaagaaa agtaataata
     2521 aaattgacct tttcctgtat cagaagctgc tagaatatct cggattgacg tttcatgata
     2581 gcctttttta taaataagat ctttggtcgc ttccaagatc ttttcctttg ttttcaatga
     2641 tattctcctt tttcatttct catagtccta tattatacag taaattcatc attttttctt
     2701 cttaatcgtt taaaaaagac tgtgccaaat tatctgacac agccctcact ataaaactgt
     2761 tctcatatag ctagatgttc attgattcaa gaactgcgag gacaaaagca attcctatct
     2821 tatgatagtt gctctaatac ttcctctgtt gtcatcactt gatctgcaat aaagccaaag
     2881 ttcgtcaacg ctgcatccat tgcttcttgg ccaggagcac caattgcatc tgtcgctacc
     2941 acaacattga atccactctc taccaattca cgcatatggg aatcgctaca caagtttccg
     3001 ttcattccac caagaattac tgtgtcgatt ccattttttc tcaattggag cgccaaatca
     3061 ttgctttctg gtccaaagat tttatgagga gagctgatga tcgtattttc atccaaataa
     3121 ggtttcaatt cttcgataaa atcggctcct gaaccttctt ccactgattc atattgactt
     3181 tttctagcaa acatctgctg ctcaagcatc atatgttctc ctgcaccgcc aaattgccat
     3241 ttatggtcat gtgggtaaaa ataatgagga gaaataaaga gtttgtatcc tttttctttt
     3301 gcttgtttta ccatttcaat catattttcg atcgtattgt atttttctaa gatatctttt
     3361 gtcaaaccaa agccaccgcc tgctggtttc aagaactcga cttgcgggtc agtgagaact
     3421 actgctgtat ttttattatt gaacatggtg gattctccat tcgttattta ttttaataca
     3481 tcatttagag cttcactcat tgtcggatga gaatagattt gatctctcaa taacgtataa
     3541 ggaagttttc cattcatagc tagcgaaatc aagttgatga cttcatgaga atcttccgca
     3601 tagatacttg cccctagaat caagtcagtt tcagggtcga ctaaaacttt gaatacacct
     3661 ttaggatctt ctaatacttg tgctttaggg acaccgcttg tcatatactt gaataatttg
     3721 taattgacgc cttgtgcttt ggcttgtttt tctgttaaac ctacattgga taaagtcgga
     3781 gtgataaata cactgtaagg cacagctttc cggttattcg ttgttcgttt attctcgccc
     3841 ttcaactgat ccataatgat tcgataatca tctagagaga tataagtgaa ctgcaatccg
     3901 cctttgacgt ctccaatcgc ccaaatgttg tctgcatctg tacgaaggta ttcatccaca
     3961 gcgattgctc cgcgatctgt tagtttaaca tctgtatttt ctaatccaag tgatgctgta
     4021 cttggttttc ttccagttgc agccaaaact tttgacgctt ggattgttac ttcttggcca
     4081 tcttctgtaa aggtgacggc agctgttgat tcttggtctg ctacttgatc tacagataca
     4141 cctagatgga attcgattcc cgcatttgtc atgtcttcta aaatcatttg cgcgatatct
     4201 tcatcttcac gtggaagaaa ttcagaatgg gcatcaagaa cgacaacttt agatccgtat
     4261 tcattgaaca ttgatgcaaa ttccaagcca atatatccag ccccgatgat cactaatttt
     4321 tccggtaatt gttcctgatc catcgcttgc gtagaatcaa tcaagtagcg acttgtattc
     4381 aatcctttga ttggtaaaat gattggttca gaaccagtat tgatgaaaat ccgttcgcct
     4441 tcaacaatca ctttttcacc attattcgtc acttcgattt gatggttaga aagaaaacgt
     4501 gctgtaccgt ccatcactgt cccagttgcc tcatcgctga tcatatgata atttttggcg
     4561 cgaagcatgc ccgtcaattt ttctttccga gcaacagcct ctgtaaagtc gattcctctt
     4621 tctccattga aaatcaatga tttagaagga atacagccga tgttgataca tgttccgcca
     4681 tacatacgtg ttgacttttc aacgactaaa accgattcac cttttgaagc taacgccttc
     4741 gctaaagtct taccgccctt tccaaaaccg atcacaatat tttgatattt ttccatcatt
     4801 tttccctcgc ttctttttta cctaccgatt ggtacaaaaa tatgataacc gataattgtc
     4861 tattctccaa atcatctgct ttatttgtca aaaaaaacca gacaaaaagc ggtcctttca
     4921 cgtttttagt ctggttcgat ttcctttatt cttctaaaca atcgttactt ctacatcgat
     4981 atttcctcta gttgctttgg agtaagggca gaattcatgc gcatcttctg caattttttg
     5041 ggcttcttct tcgctgatgc catcgatatg cacttctaat actacaccga tctgatagcc
     5101 gccatcatct aaattataaa gagagacacg tgcttttact gtggactctg ctgtcattcc
     5161 tgcttctttg atcatatgtt gtaccgcccc attgaaacaa gaagcataac ctgctgcaaa
     5221 taattgttct ggatttgttc cttctgcatg gattcctgga ggagtgattt tcatatgcat
     5281 cgagccattt ggtgcctcga cttctccatt gcgcccgcca tggttgatca ttgctgtttc
     5341 gtatgctttt ttcattgtac tgctcctttc gattgaccat ttgctacttg gctataatta
     5401 tagtgtatga caagtaaggc gctttctaca aaaattctgc ttattcgaat accagaatgc
     5461 cgtcttctac tatttttcca aaaaacaaaa aaatactact cgtgaacaac agcgtcatat
     5521 ttgttccaaa ataaatagca atcttttcta cataatcttt tatatgattg attttaagat
     5581 acatatatac cagaaagttg accccataaa ccaatactgt taaaaaaata accatattcg
     5641 ttaccatttt ttcctccttt tgctggtctc cctaccagca ctcgatttaa ccataccact
     5701 ttttttgccg tccaagcaac tagcctttca aaaagactgg tttattctgc tgttaatgat
     5761 tcaagatcag aaaaatgtcc gaataatttt gctaatttta ctaacgccaa cttgttcttt
     5821 ccgaattcat cattttctat ctgtaatact agtaacggct catgtaagct taaacggatc
     5881 aaaaaccagc cgtttccgta tggctcttct agatcatatc gaataccttc ttcattttcc
     5941 gggtcgaagt tcatcccttt ttcttcttcc aacagtcgct tcatttcttc taatatcttg
     6001 tttccttgtt cgcgtggttc atcgctgaac aattttaatc ggatttcttg tacttctacg
     6061 ggctgtttca actctttgat ttgttcatct aatgtgatac cttttctttt taattctggc
     6121 aaaagcatca ggatctttgc gatgacatat gcaccatcat ccagaaaata gttttccttg
     6181 aatgcagcgt gtccgcttgt ttcgatggct agctgtgtat ctttcccaag acggttaagt
     6241 tctaatgctt tattgattac atttcgatag ccggaaatat aacgaacttg gatgcctcct
     6301 ttttcttcaa tgaatctttg tagatgagaa gaagtagggg aattcgtcac gattcttgtt
     6361 cctggatgct cttttaatac gattgtactt aatactgcga tcaaattatt tcgattgatc
     6421 actgttccag aagcactgac aactgcagaa cgatccacat ctgtatcgaa aatgatccca
     6481 agatctgctt catttgctaa aacagcctgc ttgatacttt ccatcgcttc tttattatca
     6541 ggatttggga tatgattagg aaatcggcca tctggttcca aaaattggga acctgttgaa
     6601 tccgcaccta agctattcaa tactttttca gcaaagaatc caccagcacc atttccggca
     6661 tcaacaatga ttttccaacc atttagtggt ttgctttcta gtgttccaat ccccttttta
     6721 atttttgtga ctaaatcttg cgcataaggt gtcaaaaggt ctgctttttc tacgcttcca
     6781 gtttgttcgc tttttatttt ttgctgctgt gataggataa aagcaatatc ttctttttct
     6841 gctcctcctt ctgaggtaaa gaattttatt ccattataat aatagggaag atggctagca
     6901 gtcagcataa ccgcagcatc gcacgagaac tgttcaaatt gagtggacat aaacatagca
     6961 ggcgtagtag ctaattcgaa atcgatgaca tgtatccctt gttctttcag actttggatc
     7021 aacgcctctt tcagtgctgg tccgcttatc ctactgtcat gaccaatccc aatcgtccat
     7081 tttttatttg gatcggttga tttcttcttt ttctttatcc agtttgctag tccccagccg
     7141 atctttttta ccatgtctgt cgtcaagttt acttcatatc cttcaactgg taatgcgatt
     7201 cctcgaatat ccgatccatt ttgcagcgct ttcatatctt tcatataaaa gtcctcctca
     7261 tttattccct cattttttcc agcttttctg caaatcgctg tgcttcttct tctgagcggc
     7321 aaataataat gaatgtatct acaccggcca tcgttccgac aacttcaggg taagccactt
     7381 catctagcca attcgttacc acatcggcac catttcgttc agtcaatacg ataaccatga
     7441 actgaacaga ctggatacgc aaaacctcgc gtctaattgc tgtacgcaaa cgttcttcat
     7501 ttaaagaaac agatggctgg ttaaataatg tatagcgtac tattttgttt tcattttgac
     7561 tttttactaa cttcatttct cgaatgtctc gagaaatcgt tgcttgagtc acatcggcgc
     7621 ctgcttcatg cagccgcgca agcagctctt cctgtgtccc aataggttgt tctgtaatga
     7681 tctggcggat cagtgcttgt cgctgtttct ttttcatatt agacacctct caatgttgat
     7741 tatagcaaaa gataagtaat atttcttcat attttggtat tttttacttt tattttttgt
     7801 tttcattcag gttgctaacc tttttctatt ttaacacgca aaatcgtctt cattttttca
     7861 ggtgctgatt ttcttggatc gctgatgtaa atctctttgt gttctttaga agctctttca
     7921 tagccttctt ttgcgatgaa tgcttctaat ttctcaaacg ttgctggttc ttcggcatag
     7981 ggcccgacgt gtaagatttg ggcacaaaga ccttcttcca tctttttgaa tactagttgt
     8041 tctttcaagt cttccggaag tttgctagaa cgctgaatgg cttcttgtgc tatcgcttct
     8101 gtaacaaagt caggctgtcg gatcatcaat tgataagaaa aatgttcttt tttcatcact
     8161 ggttcatgca aatacttttc ttgcagtccc cattgtcctt ccagtggata gacagtatac
     8221 atctgataat caggaatcag ccaatttttt ttaggcgaca tgcgtattgc atagctgact
     8281 gcatatagac aggctactct tcgtttaaag tcttcctgat tcgggtcgcc gatccctctg
     8341 atgctaaaga atggtttttt aggaacctct agtatttgcg ggcttttttt catactatag
     8401 ctcttgtctt tacgccattc aattttttcc atttttcttc ctccgcgaaa ttccatttag
     8461 ttatttatta tcatacctct ttttgagtga cttggctaat cgtgttgaca ctatttttca
     8521 gtttgtgcta gaataagtga agtaaataaa tcgtacagat aacacgtttc ggcgtgtcga
     8581 gagagaggcg gtttctattt ttataataga aatcactctc tttttttacg aaatttttta
     8641 gaggtgatgt aaatgacaaa acaaaaacag ctgcttttag tacctgcagt aattgcaaca
     8701 gggatgatgt cttttgcggg cgtattgatc gagacggcca tgaatgtaac tttcccaact
     8761 cttatcagac agtttgattt aacgactgca caagtccaat gggtaacaac gatttatctt
     8821 ttgatgattt cgatcattgt tccgctgtct acttatttga atcgcaattt tagtctcaaa
     8881 aaactctttt tagcttcaaa tctgcttttc ttggcaggta taatgatcga tttcttctct
     8941 cctaccttca tggtcctatt gtttggtcga ttgctccaag gtgcagcgac aggtatcgct
     9001 cttccgttga tgttccatat tatcttgaca tttgcccccc tcaatcgacg aggcgcgatg
     9061 atgggtgtcg gtactttgac aacggccatt gctccagcaa ttggccccac ttatggaggc
     9121 attatgactt cacattttac atggaatcat atcttcctgt ttcttgtgcc cgttttgatt
     9181 ctttctttga ttatcggctg gtttgctatt cccgagatgg cagtacaaaa aagtggttat
     9241 ttggattgga taagcgtttt aggaatttgt cttttgttta gtggaagtct aacatttttg
     9301 agtacgttgg gaacagtttc cagctggatt gctttaatca tcggcggtat tggctggttc
     9361 cttttttact atcgaagtaa aaaagtggag catccactga tccgactgtc tattctttca
     9421 aataaaacat tccgcttgtt tttgattggc ttcttagttt atcagttttt attacttggt
     9481 gtatcttttg tcttgccgaa tttccttcag atcgttcaag gaagtagtcc ttttgtcgct
     9541 ggattagcga tgcttcccgg cgcagcggtt ggtgctttgc tttctccttt ttccgggaaa
     9601 atgctggatc aatatggtcc gaagaagcca atttttgctg ggctgattct ttctttgatt
     9661 ggttggctag cattattcct actcatagga cacgcttcag tgggtgtatt gattgcttgt
     9721 catgtgtttt atatgatcgg tatcggatta tcttacagca atatgatgac caccggaatg
     9781 aatgcactga atgaagaact acaaggagac gggaacgcaa tgttcaatac cttgcagcag
     9841 ttttctggcg ctgtggctac ttcattagta gcggtcatta tcaattacgt ccagcatcat
     9901 acttcccata actatgaagt gtctactaca ttaggatcaa aagcagcatt aggcgtcttg
     9961 ttgctgttgc ttctcgtaag ctttgctcga ttcgcctatt atctgttctt tgctaaaaaa
    10021 gcgtagattc catatacggc aggtgttgca tctgccgttt ttttgtgtga aaatggttgt
    10081 ttgtaaaagg aggctttttt atggaaaaaa ttgccattat cggtggcggg atcattggta
    10141 tgactctagc cagttactta gatacatcaa aattcaatgt tgctttattt gatgaaggga
    10201 caggacaggc aactaaagct agcgctggga ttatctcccc ttggttgtcg aaacgcagaa
    10261 acaaacaatg gtatcgtctg gctaaagacg gcgctgcttt tttccctaaa cttataaaag
    10321 atttccaact tgatcaaacg atttacgcac aatcagggac acttttgctt cgacctgaaa
    10381 aagatttagc tgaattggct gttcttgccg agaaacgaaa agaagaagcg ccagaaattg
    10441 gaaacatcga atggctttca cctgaacaga catctcaagc acttccgtta ttgaaaccag
    10501 cagtttcact gcgtatcagt ggtggtggtc gtctggatgg aaaagcatat ttagaacatc
    10561 ttggtcagcg tttagcacaa aaacaaatgg agcagatcca ggcaaaagtc actttgcata
    10621 agaagagcga ttcttggctc atcaagggac cgtttggaga atacacagct gatcgtgtaa
    10681 ttttgacacc tggcccggca cttaagcctt tattacaaga attaggctat gctgcagata
    10741 tacgtccgca aaaagggcag ctcttagcat ttcagacatc ttataagatg agtcaagact
    10801 ggcctgttgc tatgcttgac ggagaagctg atttcattcc tttcatggat ggaaagatcc
    10861 ttttaggtgc tacccatgaa aacgatcaag gctgggattt ggctcctact agcgaagctt
    10921 ataagggatt gactaagagt acagaacagt tcttgactga tccagatcag ataaaaaact
    10981 ggccatacga gttccgtgta ggtacccgtg cttatacgtc agatttttcc ccatttttcg
    11041 gcgtactgcc agatgatcca agcttactgg ctgctagcgg tctaggctct tctggcttga
    11101 ccacaggtcc ttatatcggc tatctattag ccaacttctt aaatactggt cattggtcta
    11161 cggaagatta tcaaaaggac atctccacct atattacgaa aaatccatca tagaaaaaca
    11221 gctttttcaa gtaaggatcc cgagcgatat tgcgcatgga ttacttcttg aaaaagctgt
    11281 tattttagtt caatttctga atcaaattct ccaaactttc ttttgcttca tctggcaaga
    11341 gatcttttcc tatctgagct tcaatgaatc gtaaacgaat atcaagtcgt tgtttcacca
    11401 gatttttata ggacgtatcc tcaaaagaaa caggaaaatc tggtataggt aaatagtgga
    11461 tgtcttcaaa tggaccaaac gacttgaatt ctgccgtttg ttgcacgatt ctttcaacga
    11521 ttgacaatgt cgtttcaggt ttgatttttt tatcatcgaa atatccagta ttcaggacat
    11581 agcaggctgt ttgctgtttc aaaaacaact ctcgaaaagc ttgatagtct tccctaagag
    11641 gatagacacg gaaaggatta gcaaatggtt caatgactag ctcatctagc tgtctttttg
    11701 tagtgatatt ttccgcgtct gatcgttttg ttgctaatgt tgcaccaaaa gacgctgcta
    11761 aaactgggtc attgatcttg ataattggcg gaagcgaatc atctttcatg atccaaaaaa
    11821 cagcatcgat cttttcgcta agatgatcga ctcggtcggg tgtcgcataa cgtgatttga
    11881 ctgtccgccc atttccgttt cgtatatctc ctgttactag gactttttct cctttacttg
    11941 tacatgtcac gcccacattc tgacaagtaa ggaaagaaga aatggccgga tcatcagacg
    12001 gatagtcttg tgttttatca aagtaagaag gttccaaggc agtagcaaaa ccattttttc
    12061 gagaaataac gaaagcatcg tcatgcaaga tcgaaacaga gtgaccttcc gtatgttttg
    12121 ctaaggtaat cgttgatttt ccagaaccgg acaacccaaa tacagccatc gtataggtct
    12181 ggtcattact agcatattgt ttcatcccac cgtggcaggc aataaagcca ttgcgatttc
    12241 ccaatgccca agcaagcgtc aaagtcgctt tttttaattc tccaaagtaa cgtaatccta
    12301 gcacagccgc tgtattatgg agagcatcaa acaaaatgag tccatttgga aatgtttcat
    12361 gtgtccattc gggatcacaa aagatataaa gatctccctc atcaaaggaa acagactttt
    12421 gataagtctc taagatcttc gaattcgctg gctgaaaatt cagcaaataa gaatacaaat
    12481 tgttctcaaa tccttctgga atcagcaaat gactttgcac agaaaattct tcagataggc
    12541 caacgattac ttttgtatgg taaaactggc gcttgcgact ttgatacaca gcttctctta
    12601 aaattttctc gtatttgctt tgttccttgt ctaagtcctc aaagatccgc cgagcacttg
    12661 ctgttcttcc cacaatgctt ccatcattga atactagtgt cctaacatca gctggtaaat
    12721 ctagttcttc agcatgtgag attgttaaat ctgtgatgat cgttcctgga ctcccttttg
    12781 ctagttgata agctgtcttc ctgtcaaaaa cttcatgaac attattaccg taaaatgtcg
    12841 tttctacaat cgatcgaata ctggataaca gtggatttcc acttgtgatt tcgtttaatt
    12901 cgagactctc ttttgtactc atggctcctc ctcattccct aaacaaaaaa acgttttctt
    12961 tattatacat tattcgagtt tttatttgta ctgctgcgct gtgggaagca aacgagttat
    13021 ttttcacaca aaaaaaccga gaacttgaat aaacaagttt ctcggtttca tcattttcct
    13081 tttatcggct tagtaccagc cgtttgctaa ccagaatgtt ttagcagcat cccatgaacc
    13141 ataacgagat gttacatatt gttctgctac acgttcttga ttggcagctg aatagtcgcc
    13201 gtttaagtat gaagcatcta attgataacg gccgatgtaa cgtccgttag tagctgtgta
    13261 tgaaccgcct gattcttttt gtgcgatcca ttcttttgca gagcttgaag ttggcgctgt
    13321 tgcagcagct ggggcagcag gagctacttc aactgtttca acaggtgcaa cgtatgtatg
    13381 ttcttgcgtt gctgtttcag ttgtagatgc tgtattttct gttgttttgt tttctgttgt
    13441 tgctgaagaa tcatttgaag ttgggattgt taattgttca ccaacataaa tgcggttgat
    13501 gtttgcgatc gaattttttt cagcaattgc atcgatcatt gagttgtctc ctgcaaattt
    13561 ttgtgagatc ttagataatg aatcacctga ttttactgta taaacttcat cagcgtgagc
    13621 atttgtagtt cctacaaaca ttgcagtagc accagcagca aaagttgtac ctaaaacaat
    13681 tttcttaaat gaattcatat taaactcctt tttttctccg ttttttattc tttcgacgct
    13741 attaacttta tcatttagaa atatttcata ggtaaaagaa acatgacatt taatgacaaa
    13801 ataacgaatt atttaatatt tgtaacattt aaacaaatat ttttttgata caaaaccata
    13861 aaagccttgt tgtataagca aagtaatcgt ttttctatgt ttttttat
//
LOCUS       DARBET010000065        13810 bp    DNA     linear   BCT 07-NOV-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4027
            SAMEA114569841-rid20112183.denovo.067, whole genome shotgun
            sequence.
ACCESSION   DARBET010000065 DARBET010000000
VERSION     DARBET010000065.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA114569841
            Sequence Read Archive: ERR12203666
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 13810)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13810)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001972618.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001972618.1
            Genome Coverage       :: 132x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/07/2023 13:18:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,041
            CDSs (total)                      :: 2,975
            Genes (coding)                    :: 2,908
            CDSs (with protein)               :: 2,908
            Genes (RNA)                       :: 66
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 67
            CDSs (without protein)            :: 67
            Pseudo Genes (ambiguous residues) :: 0 of 67
            Pseudo Genes (frameshifted)       :: 26 of 67
            Pseudo Genes (incomplete)         :: 28 of 67
            Pseudo Genes (internal stop)      :: 20 of 67
            Pseudo Genes (multiple problems)  :: 6 of 67
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13810
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA114569841-rid20112183.denovo.067"
                     /isolate="VRE4027"
                     /db_xref="taxon:1352"
                     /country="Denmark"
     gene            complement(221..1423)
                     /locus_tag="SCJ86_002158"
     CDS             complement(221..1423)
                     /locus_tag="SCJ86_002158"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010729379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HEG1169685.1"
                     /translation="MKTKYPCVYQDKNGRFSYLVELGTDQVTGKRIQKKGTKDSLGKR
                     FSSAREAHKEVMRIKNEYMVVNGFANYDLTYKEFMEQSYVPHYKASVQKSTWSSRECG
                     LIQITEFFGDKKLREINVRDCENYRIWLLNDSGYSQAYSSLMYGMFRKSLDYAVTLQF
                     LNENISKRTKAIPKGKSIVPYWTKEEFEEVLAKIYKDDFYEHMCFLMIWLYYMTGVRV
                     SEGLALNWNDVDFKRKKLRVHHTLEMTNQKDFIRKPYTKTESGLRIISLDDDTIEVLK
                     KWKVIQKNHGVEQFILSYTDLPLYRSTVQRIIQRYAKLAKVPLIQGKGLRHSHVSYLI
                     NEFNADILVVSQRLGHSSPEITLKHYAHLWSRNDEGIAEQMTGNIKFTCATKSKVANF
                     NGNQAVKI"
     gene            complement(1426..1620)
                     /locus_tag="SCJ86_002159"
     CDS             complement(1426..1620)
                     /locus_tag="SCJ86_002159"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002351139.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3173 domain-containing protein"
                     /protein_id="HEG1169686.1"
                     /translation="MKNTICRKDLEALGFKTHQATTIIRQAKTRMVNQGYPYYNNKRV
                     GVVPRQVIEEIIGVPLSQEE"
     gene            complement(1667..2515)
                     /locus_tag="SCJ86_002160"
     CDS             complement(1667..2515)
                     /locus_tag="SCJ86_002160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295979.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rep protein"
                     /protein_id="HEG1169687.1"
                     /translation="MQAYNQKIIETPTYIEIYDYEAIITPKRKTIENEVNLERKQKLA
                     WIYENDITLEKRKNYDELSAQGQYDSLKRKQKHYQSMRFEIARLVDTNFDNQTKFLTL
                     TFKENIQDIEYANNEFKKFIKRLNYDLYKTKKANLKYLATWEKQQRGAIHYHIILFDF
                     PFISFNRLTTIWGQGFVKINKIDVDSIENRGRYISKYFDKELELKEHKKKAFFKSRNL
                     KLPTVTKKMVKKPYDSTNQDILYTSEYIVRRPFFYNALALNELGSPEQVMDFQESTVH
                     YTKIKK"
     gene            complement(2720..3070)
                     /locus_tag="SCJ86_002161"
     CDS             complement(2720..3070)
                     /locus_tag="SCJ86_002161"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002401157.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEG1169688.1"
                     /translation="MPIKITDLSLPKETLEKEYQVVNVTRWQKEGEILGWSYECILPK
                     LRYEKISVKVKSEHPVITPEELTQQGLAIITFTNLVIVPWGRSNGQFVSYGLSATADT
                     ATLIPRKQGTTTQN"
     gene            3665..4111
                     /locus_tag="SCJ86_002162"
     CDS             3665..4111
                     /locus_tag="SCJ86_002162"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325167.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cro/Cl family transcriptional regulator"
                     /protein_id="HEG1169689.1"
                     /translation="MKDYVVQITKEVLEESKTLNKLSSKAKEEAVTILSSGIIDYIEM
                     SGEIKYGEKDRIAKMVKESIKTFDVKQDLTFNDSYLIGKLINILGTDEDTELFLSSLS
                     MIFTDKHFSTRFGGEELVEIFQSMRPALIKLYREKTPDEFYDWFEK"
     gene            4735..5727
                     /locus_tag="SCJ86_002163"
     CDS             4735..5727
                     /locus_tag="SCJ86_002163"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010748893.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HEG1169690.1"
                     /translation="MYWEEIELEKTLGPGGEIRAVQRDKKNISKWEKLKKQLRRDKWM
                     YLLLLPGIIMTFIFKYVPMYGAVIAFKDYNPMLGILESPWVGLDHFREFISSPNFTLL
                     LSNTLKISIYGLLLGFFPPIILALSLNQIINKKIKQKIQLILYAPNFISVVIIVGMLF
                     LFLSANGPINGLVEKVTGQPIMFMSNPAYFRTIYILSGIWQGIGWSSILYTATLAGVS
                     TDLYEAANIDGANILQKIWHIDLPALRPVMVISFILAAGGIMNVGYEKAYLMQTSMNI
                     PASEIISTYVYKVGLQSGDYAYSAAVGLFNMIINVVLLLIVNNITKRLNDGQGL"
     gene            5743..6627
                     /locus_tag="SCJ86_002164"
     CDS             5743..6627
                     /locus_tag="SCJ86_002164"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016608569.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HEG1169691.1"
                     /translation="MNSALSKIDRQILWLNRVIVTFLVVITIVPLIYVLVASFMDPAT
                     LINKGISFNPKDWTIQGYQRVFADDSILRGFVNSLFYSFSFSLLTVFITMITAYPLSR
                     PNLAGKKIIMTFFVITMFVGGGLIPTYLLVKNLGMLDSVWAIIVPGAINVWNIILART
                     YFSALPKELSEAATIDGANDLQILFKVMLPLAKPIMFVLFLYAFVGQWNSYFDAMIYI
                     KDPDLQPLQLVLRNILIQNQPSQDMVGANTAMAEMKQIAELIKYATIVISSLPLIIMY
                     PFFQKYFDKGVLVGSVKG"
     gene            6676..8283
                     /locus_tag="SCJ86_002165"
     CDS             6676..8283
                     /locus_tag="SCJ86_002165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323465.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HEG1169692.1"
                     /translation="MKAKWMLPIVFTSVLILGACGGSKEKSKSSPDYELENVSFPLKE
                     KVSLKMMSQSAPLAPNDPNDKLIFQRLEEETNLHIDWTNYSSDFAEKRNLDIASGDLP
                     DAIFNAAAGDYDLLNWAESGVIVPIEDLIEDYMPNLKKIFDENPEYRQMSTAPDGHIY
                     SMPWIEELGEGKESIHTVNGMAWINKEWLDNLGLEMPQTTDQLMTVLEAFKTQDPNGN
                     GEADEIPFSFINNGGNEDLKFLMGAFGLGDNDDHLVVDNDGQVQFTANKEGYKEAIKY
                     FNEMNNKGLIDKEAFEQDWNTYVAKGKEHKYGVYFTWDKANITGADDSYDVLPVLAGP
                     DGTKHVTRTNGMGFSRDRFVITSANKNLELTARWIDKMYEPLQSVQNNWGTYGDEAQA
                     NIFELKDGMLKHLPLEGTAPGELRQKTEAGGPLAVLDSYYGTVTTMPDDAKWRLDLMH
                     DTYLPYITEDNIYPRVFLEKEETDRLAKIETDMWDYIYRKRAEWITGGKIDEEWEEYL
                     AELERVGLEEWIQIKQTGYDNFLKGAQ"
     gene            8284..9768
                     /locus_tag="SCJ86_002166"
     CDS             8284..9768
                     /locus_tag="SCJ86_002166"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016621147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 32 protein"
                     /protein_id="HEG1169693.1"
                     /translation="MLVEETAILDKANRFIEMMRGKVNPQYRQNYHFSAPIGWINDPN
                     GFIYYKGHYHLFYQYYPYDSRWGPMHWGHARSSDLVHWEDLPVALAPDQPYDSGGCFS
                     GSAIERDGKLYLFYTGCQEIDGISHQHQCLAVSEDGIHFSKYSGNPVVAEKQLGENGL
                     IQEFRDPKVIVHNDGYYMVVATKSTQSTGRIVMFQSSDLYTWHFYSVLLEGTSEQGIM
                     WECPDLFHLDGRDVLIMSPIQIKKNGLEYHNISSTMACIGEVNWETGQMIVDNFHEMD
                     FGMDFYAPQTTRDPNGGRVMIAWMQMWDRTLPTHELGHLWAGSMSLPRELRVKKNRLV
                     QKPISTVYSHLVYQQGSENIAVTDTSVVFRNALSDSSYLHLVADLEKAEVFTIELLRS
                     KTDCLKLAYDTATQVFELDRQKLGRELTGAEEDPLNVRKVEVPLIDNQLVLEIFRDTS
                     SIEVFINGLEVMTATFYELEKARDILFHSIGESRIQAFETASVK"
     gene            9945..10937
                     /locus_tag="SCJ86_002167"
     CDS             9945..10937
                     /locus_tag="SCJ86_002167"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005230454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HEG1169694.1"
                     /translation="MKATMKDVAKLAGVGVGTVSRVLNNGSVKASTREKVEAAMTELN
                     YQPDFYARGLKMKHTNTVALILPTVWHPFFGEFAYHVETALERQNYKLYLCNSSGNSE
                     KEQEYIQMVSQNKVDGIIGITYSDIDEYISSNLPFVSIDRYFTEEVIYVTADNAAGGR
                     LAANELYRRGCQNVAYIGGYQETPNETKSRRKYFEIECRQLGISYKALDMLEPLDHLS
                     ERVTNFLLENPDIDGIFTINDPMALAVIDMLEKIGKNVPEDVQVIGFDGQKMTEGDPY
                     LVSTIAQPTKQMAEVAVAKLIAVINGETVEKRTVLPIEFGEGDTTKPLVRNDFA"
     gene            10989..11858
                     /locus_tag="SCJ86_002168"
     CDS             10989..11858
                     /locus_tag="SCJ86_002168"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010748897.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family protein"
                     /protein_id="HEG1169695.1"
                     /translation="MLYGGIEAGGTKFVCGIGNEQLQVIERKSFPTTTPEETMEQVFD
                     FFDKYKGNIASIGVGAFGPIDIQIESRTYGYITNTPKLAWQNYHFVGALENALSVPIY
                     WTTDVNAACYGEYMAGYGKGKNSVLYYTVGTGVGGGAVVDGKIVAGFSHPEMGHMLLQ
                     RHNEDQFEGICPFHHDCLEGLASGPAIEKRFGRKAQTISFDHPYWTIEADYLAQCVYN
                     TTLLYAPEVIILGGGVMKQPQLLELIRQSFKEKMGSYVDTPTLEEYLLTPRLADNAGL
                     IGCLALAIKKLNH"
     gene            complement(12278..13771)
                     /gene="guaA"
                     /locus_tag="SCJ86_002169"
     CDS             complement(12278..13771)
                     /gene="guaA"
                     /locus_tag="SCJ86_002169"
                     /EC_number="6.3.5.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine-hydrolyzing GMP synthase"
                     /protein_id="HEG1169696.1"
                     /translation="MITRRIREFGVFSELMSHRITAEEVKAMNPKGIILSGGPNSVYD
                     EKAFGIDEKIFDLGIPVLGICYGMQLITYKLGGKVKPAKNREYGKAELEITSAEAALF
                     QNTPEKQTVWMSHGDLVTQAPTDFDVVATSKDCPIASIQNAERKMYGIQFHAEVRHSE
                     YGNDLLRHFTLDVCQCTGDWTMDNFIDMEIAKIREQVGDKKVLLGLSGGVDSSVVGVL
                     LQKAIGDQLTCIFVDHVLLRKGEAEQVMESLGGKFGLNIIKVDAKERFLSKLAGVSDP
                     EEKRKIIGNEFVYVFDDEATKLAGEEGVSFLAQGTLYTDVIESGTETAQTIKSHHNVG
                     GLPEDMQFKLIEPLNTLFKDEVRELGTQLGMPDSIVWRQPFPGPGLGIRVLGELTEEK
                     LEIVRESDAILREEIANAGLDRDIWQYFTVLPGIRSVGVMGDGRTYDYTVGIRAVTSI
                     DGMTADFARIPWEVLQKISVRIVNEVAHVNRIVYDITSKPPATVEWE"
BASE COUNT         4231 a         2337 c         2851 g         4391 t
ORIGIN      
        1 acctccagac ttcttgatag ctcaagtata tcttgaggtg catttgttag taaggtactg
       61 ttctatgggg cgcttacgtt gaaaaacgtt gatttatcgg cattccccat ccatattata
      121 tttacgtctt attcccactc aatatgagta gcctcgtaaa gaagctatta tatcagtgct
      181 ttttcagcct attcctcatt ttggtaggga aatggtaggg ttagattttt actgcttgat
      241 ttccattaaa gtttgcaact ttagattttg ttgcacaggt aaatttaatg tttcctgtca
      301 tttgttctgc aattccttca tcattacggc tccatagatg agcgtagtgc tttaaagtga
      361 tttcagggct agaatggcct aaccgttgcg acacgaccag aatgtctgcg ttaaactcat
      421 tgattaggta gctaacatga gaatgacgta atccttttcc ttgtattagg ggtaccttag
      481 ctagtttggc ataacgctga ataattcgtt gaacagttga acgatagagt gggaggtcag
      541 tgtagctaag aataaattgt tctacaccat gatttttttg aataactttc cactttttta
      601 gcacttcaat tgtatcatcg tccagagaaa tgatccgcaa gccgctttca gtttttgtgt
      661 aaggtttccg aataaaatct ttttgatttg tcatttccaa ggtgtgatga accctcaact
      721 ttttacgttt gaagtccacg tcattccaat taagtgctaa tccttcgctt acacgaacac
      781 cagtcatgta atatagccag atcattaaaa aacacatatg ttcgtagaaa tcatctttat
      841 aaatttttgc caaaacttct tcaaattctt cttttgtcca gtaaggaacg attgattttc
      901 cttttggaat tgcttttgtt cttttggaaa tattctcatt cagaaattgc agggtgaccg
      961 catagtctaa tgatttacga aacataccgt acataagtga actgtatgct tgtgagtatc
     1021 cgctatcgtt caataaccaa attcgataat tttcacaatc tcgcacattg atttcacgaa
     1081 gcttcttatc accgaaaaat tcagtaatct gaatcaatcc acactcacga gaactccaag
     1141 tacttttttg tacgctagct ttgtagtgtg gaacgtaaga ctgctccata aattctttgt
     1201 aagttaaatc ataattcgca aatccattta caaccatgta ttcatttttt atacgcatga
     1261 cttctttgtg tgcttcacga gctgatgaga atcgttttcc tagagaatct tttgttcctt
     1321 tcttctggat cctctttcca gtcacttgat ctgttcctaa ctcaactaga taagaaaagc
     1381 gaccattttt atcttgatac acacaagggt attttgtttt catagttatt cctcctgtga
     1441 aagaggaaca cctataattt cttcaattac ttgtcggggt actaccccaa ctcttttatt
     1501 attataatat ggatatccct gatttaccat tcttgttttt gcttgtcgga ttattgtggt
     1561 agcttgatgt gttttaaatc ctagagcctc taaatctttt cgacaaattg tatttttcat
     1621 tgtataaact cactttcaaa gaaaacacta tcattgggta atagtgttat ttctttattt
     1681 ttgtataatg aacggtactt tcttgaaaat ccatcacttg ttcaggactt cccaactcat
     1741 ttaaagctaa agcattgtaa aaaaatggac gtctaactat atattcgctt gtatatagta
     1801 tatcttgatt cgtggaatca tagggtttct ttaccatctt tttggtgaca gttggtagtt
     1861 tgagattccg ggacttgaaa aaagcttttt tcttatgctc tttcaattcc agctctttat
     1921 cgaaatattt gctgatatag cgcccgcgat tttcgatact gtctacatcg atcttgttga
     1981 tcttaacgaa accctgaccc caaatcgtag ttaaccggtt aaatgagata aaggggaaat
     2041 caaacaagat aatatggtag tggattgcac cgcgttgctg cttttcccac gtggcgagat
     2101 actttaaatt tgctttttta gttttataga ggtcataatt cagtcgtttg ataaattttt
     2161 taaattcatt atttgcatac tcaatgtctt gaatgttttc tttgaaagtg agagttaaaa
     2221 atttcgtttg attgtcaaaa ttcgtgtcta caaggcgggc aatctcaaat cgcatacttt
     2281 gataatgttt ctgtttgcgt tttaaactgt catattgtcc ttgagcggat aattcatcat
     2341 agttttttct tttttcaaga gtaatatcat tttcataaat ccaagctaat ttttgttttc
     2401 gttccagatt tacttcgttt tcgatcgttt tacgtttggg agtaatgatt gcttcataat
     2461 cataaatttc aatatacgtg ggagtttcaa tgattttctg attataagct tgcaaaaatc
     2521 atcactcctt gttgcgtatt cgattgtcaa agaaccagtc attttctcct gatgttgatc
     2581 tataaatcaa gttaaggggg aaggcctgcg tagcagccct tctagcctat ggggtgttgg
     2641 gcggtcttaa cggacagaaa cggctctgtg ggcgctgccc cgccgtttcg tcctaaccac
     2701 ccaacactcc atagtatagt tagttttgtg tagtggtacc ttgttttctg ggaatcaaag
     2761 ttgccgtatc agcggttgcc gataacccat aactgacaaa ttgtccattc gagcgtcccc
     2821 aaggtacaat caccaaattg gtaaatgtaa ttatggctaa accttgttgg gtaagttctt
     2881 ccggcgtgat cacaggatgt tcagatttga ctttgacact gattttttcg tagcgaagtt
     2941 ttggtaggat acattcgtag ctccaaccta gaatttcgcc ctccttttgc cagcgagtga
     3001 cattgacgac ttggtattct ttttcgagtg tttcttttgg taaactgaga tcggtaattt
     3061 ttattggcat cacaattctc cttttttata cgaaagttta tacgatttga ttaaaaaata
     3121 tagatggcca tctacgactt gtattatata cgagaactta tacgtagtca agagattttc
     3181 taaaaaaata tatacgattt tttatacgag tgttataatg aaaaaaatga taaggaggaa
     3241 tttgaatatg tttggtttag tacaacctaa taaagtgaag gtgggccaac gaatcaaaga
     3301 aataaaagac ggaatgtctc tctcttttac agaattaggg aatcgattag gtttaaaaaa
     3361 gccgacgatc agttcctacg ttcaaggcta tgcgttggct cctaagagtg tgatcaacca
     3421 attatctagt atcagcggaa aacctgtcgg ctggttttat tttgggcatg tggaagaata
     3481 tatccgagag tatttgtttc tcattggtca gaaaaaaata gtggacgatt atcctgaaat
     3541 cattgagaaa ataaagcaag aattttatac gggcgagttc aaaaatcctg cttgggagaa
     3601 tgagctaggc tatcctttgg aggaatttat ccaagaatgt tttgtagaat attcgagtga
     3661 aatcatgaag gactatgtcg tacagattac gaaagaggta cttgaagaat ccaaaacgct
     3721 aaacaaacta tcatcaaaag caaaagaaga agctgtcacg attctttcat ctgggatcat
     3781 tgactatatc gagatgagtg gtgagatcaa gtatggagag aaagatcgca ttgcgaaaat
     3841 ggtgaaagaa tcaataaaaa cctttgatgt gaaacaagac ctgacattta atgattccta
     3901 tttgattgga aagttgatca atattcttgg tacagatgaa gatacggagc tgtttctttc
     3961 ttctttatca atgatattta ctgataagca tttttccact cgatttggtg gggaagaatt
     4021 agtcgaaata tttcaatcga tgcgaccagc gttaatcaaa ctctatcggg agaaaacacc
     4081 agatgaattt tatgattggt ttgagaagta aaataagaaa agtggcttag attacaagtt
     4141 ggtgaaaaaa tttttttcag cataacatca gtaatctata aaggggttgt tgattatact
     4201 gtagaagtta agtactcaag agattttttg tacgtaaaca aagcaaaaaa gggtgcagtt
     4261 tcactagaaa atgtactctt ttttgtgatt tttcttagtt atagagtttt tttgaaactt
     4321 taaaaatgag aaaacatatt gattttaaag agtgtagaca tagcttcttt tgctaaatag
     4381 attaaactta ctggtgagtg gtcttgaaca tacttttaaa tcacatttta ctgagtttta
     4441 ttggatttta taattcattg gaaacctcgt acttaatctg tttttatcta tcgactagaa
     4501 tctgtatata ttccagctat caatgaggcc gcagaaaagg gtgcttaaaa ataatctgtt
     4561 gtttaaaata ttattatcag aatacttaga tacaaaattc tggcacaaag taaaaaaaat
     4621 attgacttaa aaaaatggat atggtaatct ataaacgaaa atagtggtaa cgtttccaaa
     4681 atgcatcttc gttaaatttt tattttttta aacgaaaatg gaaacgtttc taaaatgtat
     4741 tgggaggaga ttgaattgga aaagacattg ggaccaggtg gtgagataag ggcggtacaa
     4801 cgggataaaa aaaatatttc gaagtgggaa aaattaaaga aacagttgcg gcgtgataag
     4861 tggatgtact tgttgctctt accaggtatc atcatgacgt ttatctttaa gtatgtgcca
     4921 atgtacggtg ctgtgattgc atttaaagac tataatccta tgctaggaat tttggaaagt
     4981 ccttgggtag gactagatca ctttagggaa tttatcagtt caccaaattt tacgcttttg
     5041 ctgagcaaca cattaaaaat tagtatttat ggtttattgt taggtttttt tccaccaatt
     5101 attttagcac tgagcttgaa tcaaattatc aataaaaaaa taaaacagaa aatccagttg
     5161 attctatatg cgccgaattt tatctcagta gttattattg tggggatgct gtttttgttc
     5221 ctatccgcaa atggacctat taatgggtta gtagaaaagg ttacggggca accaattatg
     5281 ttcatgtcta atccagctta tttcagaacg atttatattt tatcgggaat ttggcaagga
     5341 atcggctggt catccattct ttacaccgca acgctggcgg gagtcagtac cgatttatat
     5401 gaggcagcaa atatcgatgg ggcaaatatt ttacaaaaga tttggcatat cgatttgcct
     5461 gcattgaggc ctgtgatggt gatcagcttc atcttggccg ccggcggtat tatgaatgta
     5521 ggatatgaaa aagcctactt gatgcaaact tcaatgaaca tccctgcctc agaaatcatt
     5581 tccacttatg tatacaaagt cggcttgcaa tcaggtgatt atgcctactc tgctgctgtt
     5641 ggtctgttca atatgattat taatgtcgtt ttattactga ttgtcaacaa tataaccaag
     5701 cggctaaacg atggtcaagg cttgtaatga aaggagaaaa ttatgaatag tgctttatca
     5761 aaaatcgacc gtcaaatctt atggttgaat cgagtgattg taactttttt agtcgtcatc
     5821 accattgttc cgctgattta tgtactggta gcttctttta tggacccagc tactttgatc
     5881 aataaaggta taagcttcaa tccgaaagat tggacaatac agggatatca acgggtattt
     5941 gcagatgatt ccattttacg cggttttgta aattcgttgt tttactcttt tagcttcagc
     6001 ttattgactg tatttattac gatgatcact gcgtatcctt tgtctcgacc taacttggcg
     6061 gggaaaaaga ttattatgac tttttttgtc attacaatgt tcgtgggtgg tggcttgata
     6121 cctacctatt tattggtaaa gaacttgggc atgttagata gtgtatgggc gattatcgta
     6181 ccaggtgcta taaatgtgtg gaatattatc ttagcccgta cctattttag tgctttgcca
     6241 aaagaacttt ctgaagctgc tactatcgat ggcgccaatg acttgcaaat actgttcaaa
     6301 gtcatgcttc cattagcgaa accaattatg ttcgtattgt ttttatatgc ctttgttggg
     6361 caatggaact catactttga tgccatgatt tacatcaaag atcccgactt acagccgttg
     6421 caactcgtat taagaaatat tttgatccaa aaccaacctt cacaagacat ggtgggagcc
     6481 aatactgcca tggcagaaat gaaacaaatt gctgagttga tcaaatacgc aacgatcgtc
     6541 atttctagtc tgccattaat cataatgtat cctttcttcc aaaaatattt tgataaaggt
     6601 gttttagttg gttctgttaa agggtaagta ttgttcacta tttgtatgaa taaaaattat
     6661 aaaggagatt ttaagatgaa agcaaaatgg atgctgccaa tcgtttttac ttccgtacta
     6721 attttggggg cttgtggagg ctcaaaagaa aagagtaaat caagccctga ttatgaacta
     6781 gaaaatgtct cttttccatt gaaagaaaaa gtatctttaa aaatgatgtc tcaaagtgcc
     6841 cctttggctc ccaatgatcc taatgacaaa ttgattttcc aacgattaga agaagagacc
     6901 aatctccata ttgattggac aaactacagt agtgattttg ccgaaaaaag aaatttggat
     6961 atcgctagtg gtgatttacc agatgccatc ttcaacgccg ctgcaggtga ctatgattta
     7021 ttaaactggg cggaaagtgg cgttattgta ccgattgaag atttgatcga agattatatg
     7081 ccaaatctga agaaaatttt tgatgaaaac cccgaatacc gacaaatgtc tactgcacct
     7141 gatggtcaca tttactctat gccttggatt gaagaattag gcgaaggaaa agagtcgatt
     7201 catacagtaa atgggatggc ttggatcaac aaggagtggt tggataattt gggtcttgaa
     7261 atgccgcaaa ccactgatca gttgatgact gtgttggagg ctttcaagac gcaagatcca
     7321 aatggtaatg gtgaagcaga tgaaattcct tttagcttta ttaacaatgg aggaaacgaa
     7381 gacttaaaat ttttaatggg ggcattcggt ctaggtgata atgatgacca cttagtcgtt
     7441 gataacgatg gtcaggtaca atttactgct aacaaagaag ggtataaaga agccatcaaa
     7501 tatttcaatg aaatgaataa taaaggtctt attgataaag aggctttcga acaagattgg
     7561 aatacgtatg ttgccaaagg gaaagaacat aagtatggcg tgtactttac ttgggataaa
     7621 gcaaatatca cgggtgcaga tgatagttat gacgtcttgc cagtcttagc gggaccagat
     7681 ggaacgaaac atgttactcg tacgaatggc atgggtttct caagagatcg tttcgtaatt
     7741 actagtgcga ataaaaactt ggaacttaca gctaggtgga tagataaaat gtatgaaccg
     7801 ttacagtctg tccaaaataa ctggggaacc tacggtgatg aagcacaggc gaatattttt
     7861 gaattaaagg atggtatgtt aaaacatttg cctttagagg ggacagcacc gggggaacta
     7921 agacaaaaaa ccgaagctgg aggaccatta gcagttcttg attcttatta cggcacagtc
     7981 actactatgc cagatgatgc gaagtggcgt ttagatttga tgcatgacac gtatttgcca
     8041 tatattacag aagacaatat ctatccacgt gtctttttag aaaaagaaga aacagatcga
     8101 ttagcaaaaa tcgaaacgga tatgtgggat tacatctatc gcaaaagagc agagtggatc
     8161 accggcggta aaatcgatga agaatgggaa gaataccttg ctgaattgga acgtgtaggt
     8221 ttggaagaat ggattcaaat aaaacaaact ggctatgata attttttgaa gggagcacaa
     8281 taagtgttag tagaagaaac ggcgattttg gataaagcaa atcgatttat tgaaatgatg
     8341 cgaggaaaag ttaaccctca gtatcgacaa aactatcatt tttccgcacc catcgggtgg
     8401 ataaatgacc ccaatgggtt tatatattac aaagggcatt accatctctt ctatcaatac
     8461 tatccttacg atagtcgatg ggggcccatg cattgggggc atgcgcgcag tagtgatttg
     8521 gtccattggg aagatctacc agtagcgtta gccccagatc aaccttatga cagcggcggg
     8581 tgtttctcag gaagtgcaat tgaaagagac ggaaaactat atttatttta tacaggctgt
     8641 caagaaattg atggtattag ccatcagcat caatgtttgg cggtctctga agatggtatt
     8701 cattttagca aatacagtgg taatcctgtg gtagcagaaa aacagctagg tgaaaatggg
     8761 ctaatacaag aatttcgtga tcctaaagta attgttcaca acgatggtta ctatatggta
     8821 gtagctacta aatctacaca gtctactggt agaatagtga tgtttcaatc gtcagatttg
     8881 tatacttggc acttttactc tgtgctgcta gaagggactt cagagcaagg aataatgtgg
     8941 gaatgtcctg atttattcca tttagacgga cgagatgtct taatcatgtc tccgatacaa
     9001 atcaagaaga acggactgga atatcataat atcagttcta ccatggcttg tattggtgag
     9061 gtaaactggg aaacaggtca gatgatagtt gataatttcc atgaaatgga ttttggaatg
     9121 gatttttatg caccacaaac aacaagagat cctaatggtg gacgggtgat gattgcttgg
     9181 atgcagatgt gggatcgtac attgcctaca catgagttag gccatctttg ggcaggctct
     9241 atgagtttgc cccgagagtt aagagtgaaa aaaaatcgat tggtacaaaa acccatctca
     9301 actgtatata gtcatttagt ttatcaacaa ggcagtgaaa atattgcagt gactgacaca
     9361 tcagtcgtat ttcgaaacgc gctgtctgat tcttcatatt tacatctagt cgcagatttg
     9421 gagaaagctg aagtttttac aattgaatta ctgcggtcta agacggattg tctcaaacta
     9481 gcctatgaca ctgctacaca ggtttttgaa ttggatcgtc agaaactagg aagagagctt
     9541 acaggggcgg aagaagaccc tctgaatgta cgaaaagttg aagtaccttt gattgataat
     9601 cagctcgttc ttgagatttt tagagatacc tcatcaattg aagtctttat taatgggtta
     9661 gaagttatga cggctacttt ctatgagctg gaaaaagctc gagatatctt gtttcattcg
     9721 atcggagaaa gtcgtattca agcttttgaa acagcgagtg tgaaataaat gtttgattgc
     9781 ttaggcgatg aatcaattat ttaataggtt ttgaaggata atgtttttag aaaagaacag
     9841 atagctagat ttcccatgga tttgctatta gtattttgaa aaaattagta atttatgaca
     9901 aaattcatta tactaaactt gtgcagaaat gtgagggatc aactatgaaa gcaacaatga
     9961 aagatgtcgc aaaattagca ggtgttggag taggaactgt ttcccgggta ttgaataacg
    10021 gttctgttaa agcttctact agagaaaaag ttgaagcagc aatgacagag ttaaactatc
    10081 aaccagattt ttatgcccgg gggttaaaga tgaagcatac gaacacggtt gctttgattc
    10141 tacccacggt atggcatcca ttttttggcg aatttgccta ccatgtagaa acagcattag
    10201 aaagacaaaa ctacaagctt tatttgtgta actccagtgg taatagtgaa aaagaacaag
    10261 aatatatcca gatggtcagc caaaataaag tagacggcat cattggaatt acttattcag
    10321 atatcgatga atacatttcg agtaatctgc cttttgttag tattgaccgt tatttcactg
    10381 aagaggtgat ttatgtgacc gctgacaatg cggccggtgg acgtttggct gcaaatgaac
    10441 tttatcgccg aggatgtcag aatgtagcat atatcggtgg gtaccaagaa acaccgaatg
    10501 aaacgaagag tcgtcgaaaa tattttgaaa tcgagtgccg tcaactgggg atttcctata
    10561 aagcgttaga tatgttagaa cctttagacc atctctcaga acgtgtaaca aattttttgt
    10621 tagagaatcc cgatatcgat gggattttta cgatcaacga tccaatggca ttagctgtca
    10681 ttgatatgct tgaaaaaata gggaaaaacg taccagagga tgtccaagtt atcggatttg
    10741 atggtcaaaa gatgacagaa ggagatcctt atctagtctc aacgattgcc caacctacaa
    10801 agcaaatggc ggaagtggca gtggcaaaat taattgctgt cattaatgga gagacagtag
    10861 aaaaacgtac cgtattgccg attgaatttg gcgaaggtga cacaacgaaa cctttagtga
    10921 gaaacgattt tgcttagtgc aaatacgttt ctttttttga aaatgagtga atgaaaggtg
    10981 tagataaaat gttatatggt ggaatagaag ccggtggaac aaagtttgtc tgtgggattg
    11041 gtaatgaaca attacaagtg atagaacgga aaagttttcc taccacaaca ccagaagaaa
    11101 cgatggagca agtgtttgat ttctttgata agtacaaagg aaatatcgca tcaattggtg
    11161 taggagcatt tgggcctatt gatattcaaa tcgaatcaag aacttacgga tatatcacca
    11221 acacaccgaa gttagcttgg caaaattatc attttgtagg agctttggaa aacgccttgt
    11281 cggttcccat ctattggact accgatgtaa atgctgcttg ttatggagaa tatatggcag
    11341 ggtatggaaa aggtaaaaat agtgttctgt attacaccgt tggtacaggt gttggtggtg
    11401 gtgctgtagt ggatgggaaa atagtcgcag gttttagtca tcctgaaatg ggacatatgc
    11461 tcttacaacg tcataatgaa gatcaatttg aaggaatctg tccttttcat catgattgtt
    11521 tggaagggtt agcatcagga cctgcgattg aaaaacgctt tggaagaaaa gcccagacta
    11581 tctcatttga tcatccttat tggacgattg aggcagatta tcttgctcag tgtgtgtata
    11641 acacaacgtt actatatgca ccggaagtaa taattttagg cgggggtgta atgaaacagc
    11701 cgcaactact cgagttgatt cgccaatcgt ttaaagaaaa aatgggttcc tacgtggata
    11761 cgcctactct cgaagaatat ctactaactc caaggcttgc tgacaatgcg ggcttgattg
    11821 gctgcctagc tttagcaata aagaaactca atcattagaa tagtatttat tagaactatt
    11881 cataacatac cggaagttta aaagggtatt ctgaaactta aatagtctca attcattctt
    11941 ctttcttgag aatctgagta gagactttta ttctgtttca aattaatata attacgaaat
    12001 gatattgagt acaagaaagt tctttttacc agatctttta aatcgaaaaa tctgatatat
    12061 atatttatag tactaaaaat ctgattagat aaaaagctaa ttcaatagga aaataaaggt
    12121 agtgaataaa agcaaagccc ttgttagtag ttaatctaac aagggctttg gtagggaaaa
    12181 tggtaggaaa ccatcttatt ttttacgaaa atcaggaact ttagtaattg aataatgttg
    12241 atttataggt attcctcatc catattatgt tggctcttta ttcccactcc actgttgcag
    12301 gtggtttgct ggtaatatca tacacgatac ggttaacgtg tgcgacttca tttacgatac
    12361 ggacactgat tttttgcaag acttcccaag ggatacgggc aaagtcagcg gtcatgccgt
    12421 caatagaagt gacagcacgg atacctacag tgtagtcata cgtacggcca tctcccataa
    12481 ccccaacaga gcgaatgcct ggtaggacgg tgaagtattg ccagatgtcg cgatctaaac
    12541 cggcattggc gatttcttcg cgcaagatag cgtctgattc acggacgatt tctaattttt
    12601 cttctgtcaa ttcaccaagg acacgaatac ctaagcctgg tccagggaat ggttggcgcc
    12661 aaacgatgct gtcaggcatg cctaattggg tacctaattc acgtacttcg tctttgaaca
    12721 atgtgttcaa tggctcgatc agcttgaatt gcatgtcttc aggtaatcca cctacattgt
    12781 gatgtgattt gatggtttgg gcagtttcag ttcctgattc gatcacatcg gtataaaggg
    12841 ttccttgtgc aaggaaagaa acgccttctt caccggcaag tttggttgct tcatcatcga
    12901 agacatatac aaattcattt ccaatgattt tacgtttttc ttctggatca gacacgccag
    12961 caagttttga taagaaacgt tctttggcat cgacttttat aatgttcaag ccaaattttc
    13021 cgccaagact ttccataact tgttctgctt cacctttacg caacaatacg tgatcaacga
    13081 agatacatgt taattgatcg ccgatcgctt tttgtaaaag aacaccaaca acagaggaat
    13141 ctactccgcc tgaaaggcct aataaaactt ttttgtcacc gacttgttca cgaatttttg
    13201 cgatctccat gtcgatgaag ttatccattg tccagtcacc agtacattgg cagacatcta
    13261 gtgtaaaatg gcgcaacaag tcatttccat attcggaatg gcgaacttct gcatggaatt
    13321 ggattccgta cattttgcgt tctgcatttt ggatcgatgc gatggggcaa tctttgctgg
    13381 tagcaacgac atcgaaatca gtcggtgctt gggtaactag gtcaccatga ctcatccaaa
    13441 cggtttgttt ttcaggagtg ttttggaaaa gggccgcttc tgcagaagtg atttccaatt
    13501 ctgcttttcc gtattcacgg tttttggctg gtttaacttt tccgcctaat ttataagtaa
    13561 tcaactgcat accgtaacag attcctaaaa ctgggatccc caaatcaaaa atcttttcat
    13621 caataccaaa ggccttctca tcataaacac tgtttggtcc gccagaaagg atgattcctt
    13681 tagggttcat tgcctttact tcttctgcgg taatgcggtg gctcatcaac tctgagaaaa
    13741 caccgaactc acgaatacgg cgagtgatca gttggttata ctgactaccg taatcaagta
    13801 cgataatttt
//
LOCUS       DARBET010000066        13467 bp    DNA     linear   BCT 07-NOV-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4027
            SAMEA114569841-rid20112183.denovo.068, whole genome shotgun
            sequence.
ACCESSION   DARBET010000066 DARBET010000000
VERSION     DARBET010000066.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA114569841
            Sequence Read Archive: ERR12203666
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 13467)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13467)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001972618.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001972618.1
            Genome Coverage       :: 132x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/07/2023 13:18:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,041
            CDSs (total)                      :: 2,975
            Genes (coding)                    :: 2,908
            CDSs (with protein)               :: 2,908
            Genes (RNA)                       :: 66
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 67
            CDSs (without protein)            :: 67
            Pseudo Genes (ambiguous residues) :: 0 of 67
            Pseudo Genes (frameshifted)       :: 26 of 67
            Pseudo Genes (incomplete)         :: 28 of 67
            Pseudo Genes (internal stop)      :: 20 of 67
            Pseudo Genes (multiple problems)  :: 6 of 67
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13467
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA114569841-rid20112183.denovo.068"
                     /isolate="VRE4027"
                     /db_xref="taxon:1352"
                     /country="Denmark"
     gene            139..438
                     /locus_tag="SCJ86_002170"
     CDS             139..438
                     /locus_tag="SCJ86_002170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323300.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEG1169697.1"
                     /translation="MDYTVEFDSELLKQFPQEEHFMYKEAISHKVTEYLEHNFFRIKP
                     VRTRSAKGIYEMKVKINKKNYRIAFALKKNHVFIFYISSNLQKIVFDKEVSKKIK"
     gene            complement(535..969)
                     /locus_tag="SCJ86_002171"
     CDS             complement(535..969)
                     /locus_tag="SCJ86_002171"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295325.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEG1169698.1"
                     /translation="MNIDSLMIDEISELYKSVGWTNYTKDTARLARAFEQSESLIKRN
                     GEGKIIGIVRWITDFATIAFIQDILIHPRYQRQGIGKVLLNEALEKITSYGPVQIELL
                     TDDTEKTKKFYESVGFVQVKEMGGISYVIDTRRKKVLFGNDK"
     gene            complement(1201..1911)
                     /locus_tag="SCJ86_002172"
     CDS             complement(1201..1911)
                     /locus_tag="SCJ86_002172"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629987.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-ribulose-5-phosphate 4-epimerase"
                     /protein_id="HEG1169699.1"
                     /translation="MSCEEKIAAMKQRVLEANLALPRYGLVKLTWGNVSEVDRELGVI
                     VIKPSGVRYECMQADQMVVTDLSGNIIEEDSLKPSSDLPTHVVLYQTFEDITAITHTH
                     STHSVMWAQAGRDLPAYGTTHADAFYGKVPCTRQLTKEEVREAYEVHTGNVIVETFKE
                     RKLDPNEVPGVLVYGHGPFTWGDSPMKAVENSLILDEICLMAKENELINPNICEIPQY
                     LLDKHYFRKHGKYAYYGQ"
     gene            complement(1901..2761)
                     /locus_tag="SCJ86_002173"
     CDS             complement(1901..2761)
                     /locus_tag="SCJ86_002173"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342714.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-ribulose-5-phosphate 3-epimerase"
                     /protein_id="HEG1169700.1"
                     /translation="MAQIGIYEKALPKDISWKERFSLVKEMDFDFIEMSIDETDERLA
                     RLDWSEEKIAELREEMFSSGVRIHSICLSAHRRFPFGSADPEKKKAAKQLMKKAIHLA
                     HNLSVKVIQIAGYDVYYEEKSMNSREDFIEGIKESVKEASKYGIILSVEIMDDPFMNS
                     ISKFLEIKEQIHSPFLQVYPDLGNLSAWPENNPARELEKGIDYITAIHLKDTYPVTKE
                     YSGQFRDVPFGSGCVDFLGLLRHLKRLDYDGTFLIEMWSEKSQDFRREIQQAKDYLSS
                     KLKEAGYDVL"
     gene            complement(2766..3404)
                     /locus_tag="SCJ86_002174"
     CDS             complement(2766..3404)
                     /locus_tag="SCJ86_002174"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345878.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-keto-L-gulonate-6-phosphate decarboxylase
                     UlaD"
                     /protein_id="HEG1169701.1"
                     /translation="MSRPNLQIALDHNDLEHALGDVMKVGDVVDIIEVGTILCLQEGQ
                     KAIRCIRSMFPDKKLVADTKCADAGGTVASNVAKAGADWMTVICCATIPTMEAAQKEI
                     GELQVELYGNWTFEQAMDWHNIGIRQVIYHQSRDALLSGETWGEKDLSKIKKLIELGF
                     NVSVTGGLNPHTLHLFEGIDVYTFITGRGITAANDPMKAAQNFKDEIIRIWG"
     gene            complement(3419..3718)
                     /locus_tag="SCJ86_002175"
     CDS             complement(3419..3718)
                     /locus_tag="SCJ86_002175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623948.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HEG1169702.1"
                     /translation="MRILVSCANGSGTSLMMMRSVEKAMKELNVPITKIHHCAISEGK
                     SSAGQYDVVFTPVNFLNMFTQAEKKGVTVIGIKNVMSVKEISEKFAETKWYQKNK"
     gene            complement(3761..5245)
                     /locus_tag="SCJ86_002176"
     CDS             complement(3761..5245)
                     /locus_tag="SCJ86_002176"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS ascorbate transporter subunit IIC"
                     /protein_id="HEG1169703.1"
                     /translation="MGEFLLSIWTYFATNILTQPAYLIGFIVLLGYILLKRPLHECIA
                     GFLKATVGYFILTVGSGGLVNNFRPILVGLKERFNLDAMVTDPYFGQNAVDAGLMETF
                     GRTFGDVMILLLIAFVLNILLVRFQKYTKLRSVFTTGNVQVQQAATAFWILLFCFPDL
                     GRIQALIFMGLILGCYWTVASNLTVGITQELTEGAGFAVAHQQMFGIFIFAKLAEWMK
                     KRDEKKNRSIKQDKKIEDIKLPGFLSIFNENMVATSLLMLFFFGIILIVLGKDYLIQA
                     QFMQEGQSFLFYIMTTSLNFAVYLAILQLGVRTFVDELTQSFQGISNTILPGAVPGID
                     VAATFGFGSPNAVTIGFLFGALGQFLMIGLLILLKSPTIVIAGFIPLFFDNAVIAVYA
                     NNRGGFKAACLFPFLSGIIQVGGSALFATWIGMSTYGGYIGMFDWATAWPLFTVIMKV
                     LGYAGIAVVVIGLLAIPQLQYRKNPDGYFLIVEDYDTYAEKQRV"
     gene            complement(5265..5726)
                     /locus_tag="SCJ86_002177"
     CDS             complement(5265..5726)
                     /locus_tag="SCJ86_002177"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002372910.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HEG1169704.1"
                     /translation="MLKYFFDHQLIHYPDKEITDWREAIAESCLVLIDKEIIDETYVE
                     EIIHCVEKYGPYIVIAPQVAMPHSSEKSDGVFGTAISFTKLKQPIRFESEGENEEKTA
                     SLFFTLAAKDPEEHLENIQKLSELLMTDGLVERLISTNSIDDYQVVMAEYN"
     gene            complement(5744..6811)
                     /gene="ulaG"
                     /locus_tag="SCJ86_002178"
     CDS             complement(5744..6811)
                     /gene="ulaG"
                     /locus_tag="SCJ86_002178"
                     /EC_number="3.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323295.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-ascorbate 6-phosphate lactonase"
                     /protein_id="HEG1169705.1"
                     /translation="MSKTVNEVTKEKWLVETFPEWGTYLNEEIETEVVDPGTFAMWWL
                     GCTGIWLKSHEGTNILCDLWCGTGKRSHGNGMMKKGHQMMRMSGVQNMQPNLRTQPFV
                     IDPFAIKNVDALIVTHIHSDHLDIHTAAAVHQNCPEATFIGPKEVVRTWQKWGIPEEK
                     TITVKPGDAVKIKDINVVALEAFDRTALVTCEDPEEVLKGKMPQEMDQIAVNYLFETS
                     GGNLYHAGDSHYSNLFAKHGNEHKIDVCLGAYGENPRGITDKVTSVDMLRMAESLNAK
                     VVIPVHYDIWSNFMADPKEITEIWKFKKDRLKYGFKPYIWQVGGKFIYPTNKDDLEFN
                     FYRGFEDVFSIENDTPFPSFL"
     gene            7029..7799
                     /locus_tag="SCJ86_002179"
     CDS             7029..7799
                     /locus_tag="SCJ86_002179"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR/GlpR transcriptional regulator"
                     /protein_id="HEG1169706.1"
                     /translation="MKNSLVNIEKRQKEILELLQQTEQLSTFELAESLDVSISTIRRD
                     LNLLEEKNEIIRNYGYCSYNYKNQTDFDQSGPERIKQAIAREASKYINDYDTLFINSS
                     STALKTLDFLQSNHLTVITNNVKINYSPHKDNCSYILTGGEMRFPKEALVGDIALNTI
                     SSVNADICIIGCSGVDVVHGVTTKILNESTINEMMVNKTIKCKILVADHRKIGLTSKF
                     KIADISVFDYLITDKFCSEKVLEEIRNTGIKTIQVDSF"
     gene            complement(8072..8899)
                     /locus_tag="SCJ86_002180"
     CDS             complement(8072..8899)
                     /locus_tag="SCJ86_002180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323294.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HEG1169707.1"
                     /translation="MIKVDGALLKEYRKKKGLTQVQLAEGICEQATVSNIENKNMSPG
                     INILSALCKKLNVEISDILVETEEDKIRKKINEIQSLNSVSKYQESYKILQTIDQEKV
                     SNSDLLNQILFYQGLLDHLYAKKPDAALFYFNQVLEQTTETDIHHLQAAANVAMIYLS
                     KGELDFAKVYVERTLKILSETDFDNLMVCIVYYDIATYYRKIEDYDKAIQLCEKGIEY
                     NKKHKSTYALEYLLYEIASCHKQLGEDDYLERYMDAKKIARFNGNDYAVKVIENDLK"
     gene            9467..10702
                     /locus_tag="SCJ86_002181"
     CDS             9467..10702
                     /locus_tag="SCJ86_002181"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336948.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyl transferase"
                     /protein_id="HEG1169708.1"
                     /translation="MKTILFAPAVFNLAETTRMIEVAKAMKDRYYCVFFGFSKTFAPL
                     ITESGFEYHLLAPILTKKQEEQIIKFDQLRTLKNPFTKEMVASRITSEQSLIAKFEPR
                     LIVTGSNVTIFLSARNKKIPLVYVKPYALSEPFFQSEHALMPHELRFFGPLSNPLWHW
                     TKKRIVNTRWIPNAFKHAAQKEMLPLPKTSLQMMDADLNLITTPKLFLEEEQLPENYR
                     IVGPIFAKLSTSLPEEVLTFIQRKQQERKRIIYFAMGSSGNPRMLRKILFFLQKQPDL
                     AVIAPIDYLLDDIQSDSEDLLITEYLSSHLLHEFIDFSFIHGGEGTVQTACESGKPFI
                     GVGMQFEQSCNIDYCVKYGNAIALTKPVTERKLQIALREVSSPKIRQQARTLQAVFPI
                     NGPQQAIQEIETFLSNHPI"
     gene            complement(11701..11961)
                     /locus_tag="SCJ86_002182"
     CDS             complement(11701..11961)
                     /locus_tag="SCJ86_002182"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327982.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diacylglycerol kinase"
                     /protein_id="HEG1169709.1"
                     /translation="MYWLILFFVFIFLLTASHLILNMLATYHIQINRWIWALASFLIV
                     ILPKIIVPHMNVLFSWGTYVLCGIFAINFMIEQHRWFVTSKL"
     gene            complement(11988..13289)
                     /locus_tag="SCJ86_002183"
     CDS             complement(11988..13289)
                     /locus_tag="SCJ86_002183"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HEG1169710.1"
                     /translation="MEKLMKWVEEKLVPPMAKIGTQRHLLAIRNGVVSTLSLILIGTF
                     FMVFINLPFPGWNEFIAPYSATIVLPFRMTMGLMAIYATFVMGSDLAKSYGLDSVTGG
                     ILSLGTFFMLQVPVNVLTPEEAPLGWVLPMSSLGASGMFAGILSMIFAVEVYRFFKNR
                     NITIKMPEQVPPAVARSFEALIPGAVILTITWLIRSVAGFDVNAALLSLFEPLTNILG
                     NNLLGVLLPMFLIHLLWSFGIHGMSIVGAVVRPMWLIMLDENAKALADGTAATQLPYI
                     APEQFYQWTVTIGGAGATIVVSVLFIFFCKSKFLKEVGRFSIIPGIFNINEPIIFGAP
                     MMLNPYMFIPFNLVPLVLTIISYFAVKLEMVNRFTVLPTWTLPAPIGGYLSAGNDWRV
                     VILIMINTLIAFIIYYPFVKAYDKKMLSDEQSRGENEVQTV"
BASE COUNT         4294 a         2784 c         2109 g         4280 t
ORIGIN      
        1 tcaatatgta tcctggaatt ggagatctcg tagagctata tcaagatgaa tcaaatatac
       61 ttgtcattca tcatgaaata tagagactta taaaattgtt tatttttata atgtatcagt
      121 atagaaagga aataaaacat ggattatact gttgaatttg acagtgaact acttaagcaa
      181 tttccacaag aagaacattt catgtataaa gaagcaattt cccacaaagt gacagagtat
      241 ttggagcata atttttttcg tataaagcca gtgcgtactc gttcggcaaa aggaatatac
      301 gagatgaaag tcaaaattaa taaaaaaaat tatcgcatag catttgcttt aaaaaaaaat
      361 catgtgttca ttttttatat tagttcgaat ttgcaaaaaa ttgtatttga taaagaggtg
      421 agtaaaaaaa taaaatagct gagcattgga ggatagattg agtgatacta agtctatcct
      481 cttgtttttc taaagaacta agctagaatt ttttcttttg tacgttatta tatgttattt
      541 gtcattacca aaaagcacct tttttcttct agtatctatg acataagaaa taccacccat
      601 ttcctttact tgaacaaatc ctactgattc ataaaatttt tttgttttct cagtatcatc
      661 tgtcaataat tcgatttgga ctggtccata actagtgatc ttctccaaag cttcattcaa
      721 aagcactttg cctattcctt ggcgctgata cctcggatga atgaggatat cttgaataaa
      781 tgcgatcgtc gcaaaatcag tgatccaacg aacgattccg ataatcttcc cttccccatt
      841 acgctttatc agagattcag attgttcaaa tgctctagct aaccttgcag tgtcttttgt
      901 gtaattcgtc caaccaacgc tcttatataa ttcagaaatc tcatcgatca taagggaatc
      961 aatattcatt tttttcttcc tttcttatac aattctccct ctaataatac agttctttga
     1021 ctatttaatc tatcacatat aactctcctg taaagattct ctcgattcct taaaattcct
     1081 ctttcctttt agaatgcata ctttttgata aataaaaagg ttgtgataaa ttttcttcac
     1141 aacctctgtt tctctctatc aagtgttatc ttttaatttt ctacccttac cctagcatct
     1201 ctattgcccg tagtatgcat actttccatg tttgcgaaaa taatgtttat ctagtaaata
     1261 ttgcgggatt tcacatatat tggggttgat cagctcgttt tctttggcca tcagacaaat
     1321 ttcatctaag atcaaactgt tttctactgc tttcataggt gaatcccccc atgtgaacgg
     1381 tccatgtcca tagacaagta ctccagggac ttcgtttgga tctaattttc tttccttaaa
     1441 cgtctcgaca atcacattcc ctgtatgaac ttcgtaagct tctcttactt cttctttcgt
     1501 taattggcgt gtacaaggaa ccttaccata aaaagcatca gcatgagtcg tgccataagc
     1561 tggcaaatcg cgtcctgctt gcgcccacat gacggagtgc gtggaatgag tatgagtaat
     1621 agcagttata tcttcaaaag tttgatacaa aacaacatgt gtggggagat ctgaagatgg
     1681 ttttaagcta tcttcttcta ttatatttcc cgacaaatct gttaccacca tttgatctgc
     1741 ttgcatgcat tcatatctga caccgcttgg cttaatgacg atgactccta gctcacggtc
     1801 cacttcactc acattgcccc aagtcaattt gactaatccg tatcgcggca atgccagatt
     1861 tgcttcaagg acacgttgct tcattgctgc aattttttct tcacaagaca tcatagccag
     1921 cctccttcaa tttagaactt aagtagtctt ttgcctgctg gatttctctt cgaaagtctt
     1981 ggcttttttc actccacatt tcaatcagaa atgttccatc ataatctaga cgtttcaaat
     2041 gtcttaataa tccaagaaaa tcaacacaac cgctaccaaa aggaacatca cgaaactggc
     2101 ctgaatattc ttttgttaca gggtaagtat cttttaagtg gatagcagtg atataatcga
     2161 tccctttctc aagttctcga gccggattat tttccggcca tgcggacaaa ttgcctaggt
     2221 ctggatatac ttgtagaaaa ggtgaatgaa tctgttcttt gatttctaaa aattttgaaa
     2281 tggagttcat aaatggatca tccatgattt ccacggaaag aatgatgcca tatttagaag
     2341 cttcctttac gctttctttt attccttcga taaagtcttc tctggaattc attgatttct
     2401 cttcataata aacatcgtat ccagctattt ggattacttt gacgcttaga ttatgcgcca
     2461 aatggattgc ttttttcatt agttgtttcg ctgctttttt cttttctgga tcagcagaac
     2521 caaacggaaa tcgccgatgc gcacttaaac aaatcgaatg gatacgtaca ccggagctga
     2581 acatctcttc tctcagctca gctatctttt cttcagacca atctaatcgt gctaaccgct
     2641 catccgtctc atcaatcgac atctcgataa aatcgaagtc catttctttt acaagggaaa
     2701 aacgttcttt ccatgaaata tctttgggta acgctttttc ataaatccca atttgagcca
     2761 tttccttacc cccagatacg aatgatctca tctttgaagt tttgtgcggc cttcatcgga
     2821 tcatttgctg ctgtaatacc acgaccagtg ataaaggtat acacatcgat cccttcaaat
     2881 aagtgtaatg tgtgtggatt taatccacca gttacagaaa cattaaagcc tagttcaatc
     2941 agttttttga tcttcgataa atctttttca ccccaagttt caccagataa cagcgcatca
     3001 cggctttgat ggtagattac ctgccggata ccgatgttat gccaatccat ggcttgttca
     3061 aaagtccaat tgccgtagag ttctacttgc aattcaccta tttctttttg tgctgcctcc
     3121 atagttggaa tcgttgcaca acaaatgact gtcatccagt ctgctcctgc ttttgcaaca
     3181 ttactcgcca ctgttcctcc agcgtctgca catttcgtat cagcgactag ttttttatct
     3241 gggaacatac tgcgaataca gcgaatcgct ttttgtcctt cctgtaagca aagaattgtg
     3301 cccacttcaa taatatctac tacatcacca actttcataa catctcctaa tgcatgttct
     3361 aagtcattgt gatctaacgc aatttgcaaa ttcggcctgc tcatcttagt tccttccttt
     3421 acttgttttt ttggtaccac tttgtttcag caaatttttc actgatttct ttgactgaca
     3481 tcacattttt gataccgata actgtaactc cttttttctc tgcctgagta aacatattca
     3541 aaaaattgac tggcgtaaaa actacatcgt attggccagc tgaacttttt ccctctgaaa
     3601 tggcacaatg atggattttg gtaattggta cgtttagttc tttcattgct ttttccacac
     3661 ttctcatcat catcaagctt gttcctgatc catttgcaca cgatactaaa attctcatct
     3721 taatccctcc attatgagtg ttgtctaatc agattttctt ttatactctc tgcttttctg
     3781 catatgtgtc atagtcttct acaatcaaaa aataaccatc aggatttttc cgatattgta
     3841 actgcggaat agccaataaa ccaatgacaa ctacggcaat tccagcataa cctaggactt
     3901 tcatgattac tgtaaacaat ggccaagctg ttgcccagtc gaacatcccg atataaccac
     3961 cgtatgttga catcccaatc catgtagcga ataatgctga accgcctact tgtatgattc
     4021 ctgaaagaaa cgggaacagg caagcggctt tgaatccacc tcggttattg gcataaactg
     4081 ctataacggc attatcaaag aacaacggaa tgaaaccggc tatgacgatc gttggtgatt
     4141 tcaataaaat cagtaaacca atcatcaaaa actgtcccaa agcaccaaac aagaacccaa
     4201 tcgttaccgc atttggcgaa ccaaagccaa aagtagcagc tacgtcaatt cctggaacag
     4261 ctcctggtaa aatcgtgttt gagattcctt ggaaagattg agttagttca tcgacgaatg
     4321 tccgaacacc taattgcaag atcgccagat ataccgcaaa atttaaagaa gtcgtcataa
     4381 tgtagaataa gaaactctgc ccttcttgca tgaattgcgc ttgaatcaaa taatctttgc
     4441 ctagtacaat cagaatgatc ccaaagaaaa atagcatgag taacgaagta gcaaccatat
     4501 tctcattgaa aatcgacaag aatcctggta atttgatatc ttcaattttt ttgtcttgct
     4561 tgattgatct atttttcttt tcatcccgct ttttcatcca ctcagctaat tttgcaaata
     4621 taaagattcc gaacatttgc tgatgtgcca ctgcaaatcc ggcaccttct gtcaattcct
     4681 gcgtgattcc tactgttaag ttagaagcta ctgtccaata acaacctaaa atcagtccca
     4741 taaaaatcaa tgcttgtatc ctacctagat caggaaagca aaacagtaaa atccaaaaag
     4801 cggtagctgc ttgttgaacc tgaacgtttc cggtagtgaa aacggaacgc agtttcgtat
     4861 atttctgaaa acggactaat aagatattta aaacaaatgc aatcagtaat aaaatcatca
     4921 catctccaaa ggtacggcca aatgtctcca ttaatccagc atccacagca ttttgtccaa
     4981 aataaggatc tgtaaccatg gcatccaaat tgaaccgttc ctttagtcca accaagattg
     5041 gccgaaaatt attgaccaaa ccaccagagc caacagtcaa aataaagtaa ccgaccgttg
     5101 cctttaagaa tccagcgatg cattcatgaa gcgggcgttt cagcaaaata tagcctaata
     5161 acacgataaa accaatcagg tatgctggct gcgtcaagat atttgttgca aaatacgtcc
     5221 aaatgcttaa caaaaattcc cccatctttt ttctcctcta tcaattaatt atattctgcc
     5281 atcacaactt ggtaatcatc gatggagttc gtagagatga gacgctcaac caatccatct
     5341 gtcattaata attcggataa tttttggata ttttctagat gttcttctgg atcttttgct
     5401 gcgagtgtaa aaaacaaact agccgttttc tcctcattct ccccttcact ttcaaaacgg
     5461 attggctgtt tcaacttggt aaatgaaata gctgttccaa aaacaccatc acttttttct
     5521 gaagaatgcg gcattgctac ttgaggtgcg atcacaatat aaggaccgta tttttctaca
     5581 caatggatga tctcctccac atacgtttca tcaataattt ccttatcgat caatacaaga
     5641 cagctttcgg cgatagcttc acgccaatcc gtgatttctt tatctgggta atgaatcaat
     5701 tgatggtcaa aaaagtattt cagcaagaaa ctccccttct ttcttataaa aacgacggaa
     5761 acggtgtatc attttcaatc gagaagacat cttcaaatcc tcgataaaaa ttaaactcaa
     5821 gatcatcttt gtttgtcgga tagatgaatt tcccgcctac ttgccagata taaggtttaa
     5881 atccatactt caggcgatcc tttttgaatt tccaaatttc agtgatctct tttggatcag
     5941 ccataaaatt agaccaaata tcgtagtgga caggaatcac tacttttgca ttcaatgact
     6001 ccgccatgcg cagcatatca acagaagtaa ctttgtctgt tatccctcgt gggttttcac
     6061 cataggcccc taaacagaca tcgattttgt gttcattacc atgtttggca aataaattgg
     6121 agtaatgtga atctcctgca tgataaagat tccctcctga tgtttcgaat aaatagttta
     6181 ctgcaatttg atccatctcc tgaggcattt ttccttttaa tacctcttct ggatcttcac
     6241 aagtgaccaa cgctgtccga tcaaacgctt ctaaagctac tacattgatg tctttgattt
     6301 tgactgcatc acctggcttt actgtaatcg ttttttcttc aggaattccc catttttgcc
     6361 acgtgcgaac cacttctttt ggcccaatga atgttgcttc aggacaattt tgatgaactg
     6421 ctgcggctgt atggatatct agatgatcgg aatggatatg tgtaacgatt aaagcgtcta
     6481 cattttttat cgcaaaagga tcgattacga aaggctgagt acgtaaatta ggctgcatat
     6541 tttgaacgcc actcatacgc atcatctgat ggcctttttt catcatgcca ttcccatgac
     6601 tacgttttcc cgtcccacac catagatcac ataaaatatt cgttccttca tgacttttca
     6661 gccaaattcc cgtacagccc agccaccaca tagcaaaagt tccaggatcc actacttctg
     6721 tttcgatttc ttcattcaag tatgtgcccc actcaggaaa tgtttctact aaccactttt
     6781 cttttgttac ttcattgaca gttttactca tttgcttgct ccccccatat cgttgttcca
     6841 acttacaagt aaatattaat gtgtatcagc atccatgaaa acccttttat atgaccgttt
     6901 gttttttata tgattttctt tcagtaatca atgattgttt cagtataata aagacagata
     6961 tgaatgttta aatgaacaac tagtcaaata aatccatgga tcaagatgtc cctcaggagg
     7021 caaaagtcat gaaaaactca cttgtcaata ttgaaaaacg tcaaaaagaa attttggaac
     7081 tgcttcagca gactgaacaa ctatcaactt tcgaattagc tgaatccctt gatgtatcta
     7141 ttagtacgat acgaagagat ttaaatttat tagaagaaaa aaacgagatc atacgcaatt
     7201 acggttattg ttcttataat tacaaaaacc agactgattt tgatcaatct ggtcctgaaa
     7261 ggattaaaca agcgattgcc agagaagcta gtaaatacat taacgattat gatacgctgt
     7321 ttatcaattc tagctcgact gcgttgaaaa cactggattt tcttcaatct aaccatttga
     7381 cagtcattac aaacaacgtg aaaatcaatt actctccgca caaagataat tgtagttata
     7441 ttttaactgg tggagagatg cgctttccaa aagaagcact agtgggcgac atcgcattaa
     7501 atacgatttc ttcagtcaat gcagacattt gtatcattgg ctgtagcgga gtcgatgtag
     7561 tacatggggt cacaacaaag atattaaatg aatcaacgat caacgaaatg atggtcaaca
     7621 agacaatcaa atgtaagatt ttagtcgctg atcatcgaaa aatcggactg acatcaaagt
     7681 tcaagattgc tgatatttca gtctttgatt atttgatcac tgataaattt tgttctgaaa
     7741 aagtattaga ggagatcaga aatacgggga taaaaaccat tcaagtagat tcattttaag
     7801 aaccaggacg taaatatgta aataggatta tcaaaaaaat gaataacaaa tttatcgata
     7861 aatgaaaaac acgctacgct caaattgata aaggaatccc aagatgtatc catccaatcg
     7921 ataccatcat aatctattgc ttcttttata accgttatat gttgcctaaa taggaatcaa
     7981 acaatatcga aaaaaggagg tatgacaaaa gtcacacctc cttttttcga ataaacggta
     8041 ttcaaatcag aacactgatt tcacaaccac tttatttcag gtcgttttca atcaccttta
     8101 ccgcgtaatc atttccgttg aaacgcgcta ttttttttgc gtccatatat ctttctaaat
     8161 aatcatcttc tcctaattgc ttatggcaag aagcaatttc ataaagcaag tattccaatg
     8221 cataagtcga tttatgtttc ttattatact caataccctt ctcacataat tggattgctt
     8281 tatcataatc ctcgatttta cggtaatagg tggcgatatc ataatataca atgcagacca
     8341 tcagattatc aaaatcagtc tcactcaata ttttaagtgt acgctccaca taaactttcg
     8401 caaaatctaa ttctcctttt gacaagtaga tcattgccac gtttgcagct gcttgtaagt
     8461 gatggatatc tgtttcggtc gtttgttcca acacttgatt aaaataaaat aatgctgcat
     8521 ctggtttttt cgcataaaga tgatcaagca gtccttgata aaataaaatt tgatttaaaa
     8581 gatcagaatt acttactttt tcctgatcga tcgtttgtaa aattttataa gattcttggt
     8641 attttgatac agaattcaat gattgaatct catttatttt tttcctgatt ttgtcttctt
     8701 ctgtttctac taatatatcg gagatctcga cattcagctt tttgcaaagc gcacttaaga
     8761 tattgatgcc tggcgacata ttcttgtttt cgatattgct gacagttgct tgttcacaaa
     8821 tgccttctgc taattgtact tgtgttaacc cctttttctt tcggtattct tttagtaatg
     8881 ctccatctac tttaatcaaa acaatttcct tccctttaaa aaattatttc tcctttatat
     8941 catacacaaa aaaaagttct aaacaaccaa aaatgtttag aacagaataa aaattttaat
     9001 tttaaaataa tattcaaatt aaacaactgg cgtatctgct ccttgatttc tttttgtgtt
     9061 atctgaataa ttatcgttta cagttataac acatagtgaa aattgtcatg gcgagaaagt
     9121 ctaagaatat ttttaaacat aactatttct ttcttacgtg tgtataatga cgacagcaca
     9181 cctatcaatt aatataatta ttttattttt ttatgttctc atgtttattt ttaatgacac
     9241 aacttctcgt atttatctgg cacaacatat aaatcgatca aaaaattgtt gcgtgttttt
     9301 ttgaaaattg gagtttcaac ttgattacaa aatttaaatt tagaagttgt gaaaacagcg
     9361 agagacagtt taatattcaa aatatacata ggctactcga acgctgtgtt ttttagtata
     9421 ctagtaagaa acgaagggat agactcattc caataaggag gatttcatga agacaattct
     9481 ttttgctcct gcagtattca atttagctga gacaacacga atgattgagg ttgccaaagc
     9541 tatgaaagac cgttattact gtgtattctt tggcttttct aaaacttttg ctcctttgat
     9601 cacagaaagt ggctttgagt atcatttact agcgccgatc cttacgaaaa aacaagaaga
     9661 acaaatcata aaatttgacc agttgcgtac acttaaaaac ccttttacca aagaaatggt
     9721 tgcctctcgt atcactagtg aacagagtct gattgctaag tttgagcctc gactgattgt
     9781 gacaggttcg aatgtgacaa tttttttatc ggcccgcaat aaaaagattc ctttagtata
     9841 tgttaaaccc tatgctttga gtgaaccttt ttttcaatca gagcatgcgc tgatgcccca
     9901 cgagttacgt ttcttcggcc ctttaagcaa tcccctttgg cactggacaa aaaagcgtat
     9961 agtaaatacc cgatggatcc ccaatgcctt caaacacgct gcacaaaaag aaatgcttcc
    10021 tttaccaaaa acaagtttac aaatgatgga tgctgatcta aatctcatca caacgcctaa
    10081 actatttttg gaagaagaac agctgccgga aaattatcga atcgttggtc caatttttgc
    10141 taagctcagt acttccttgc ctgaagaagt gctaactttt atacagcgaa agcaacaaga
    10201 aagaaaacgt attatctatt tcgcaatggg tagttcaggc aatccgcgta tgttacgcaa
    10261 aattttgttc ttcttacaaa aacaaccgga tctagcggtt attgcaccta ttgactatct
    10321 gcttgatgac attcaatctg actcagaaga tcttttaata acagaatatc tgtctagtca
    10381 tcttttgcat gaattcattg atttttcttt tatccacggc ggggaaggga ccgtccaaac
    10441 tgcctgtgaa tccggcaaac cgtttatcgg tgtggggatg caatttgagc aaagctgtaa
    10501 tattgactat tgtgtaaaat acggaaacgc aatcgccctc acaaaaccag tgactgaaag
    10561 aaaactgcag attgctctgc gcgaagtttc ttcacccaag attcgtcagc aggcacgcac
    10621 gctgcaagcc gtttttccaa taaatggtcc acaacaagca attcaagaaa tcgaaacctt
    10681 cctgagcaac cacccaatat agtttcaagt agaaaaagct tttttaatag agactcatca
    10741 gtaatttcgc tattcgagac ccagacagtg aaaatacaaa aaaagtaaat agtggattga
    10801 aaagttagag ttacttaaat tacggtgtct aaaaaacaca aaatcatcct taaaataaaa
    10861 gatctgacgc tatccaaatg agcataggta gcgtctgcca tttattctta gtcaaattct
    10921 ttgacacctt tctctggttg gatcatctca acgtttcttc aaaagaaaga ccataataat
    10981 taggcaaata cattttctta aaatgtgtca tatagatcta gaagcatcgc tttgtagaga
    11041 tttgtgccgc tagtcccctc aacaattact aaatcaaatg taggatttac cactttatct
    11101 ttgataatca acctccctct tctgttgaga caatgatctc gcaatccgat gcatactcct
    11161 atataaactg ccagcaatcc ccacatattg agtagatcat gtcagaatgt tctatgtcgt
    11221 cagcaacgat acactaaaag tcacgttcac catgactgat tgctattccc agcacaatta
    11281 tttctataca cgtttcatga tttgaataaa cactcacatc atacatatta ccattcatac
    11341 accatactgc agcccctact atatggttgt aagctcccaa atctatattt gacttgatca
    11401 ctttggtagt agatctacta attctaaatc cattgctgat aaaatttcca ttttttcctc
    11461 cttctttctg ccaaaacagt taaaacaata ataatcttta tgtaaaaaag ggctgtgaca
    11521 aaagccttgt cacagtccca tattccgaat aaacggtggt caaaagcttg catctgcggt
    11581 aataagttca accaaacgaa aaataagcca acatttcaca aaaatttaag aagcaatttt
    11641 cgtaaatttc ttcttatttc ttgaagctaa ccacttctgt tacaaccact tttagaacat
    11701 ttaaagcttc gatgtgacaa accatctatg ctgctcgatc ataaaattga tagcaaaaat
    11761 tccacaaagt acatatgtcc cccatgaaaa caaaacattc atatgcggta caatgatctt
    11821 tggaagaatc acgatcaaaa atgatgccag tgcccaaatc catcgattga tttgaatatg
    11881 atatgtagct agcatattca atataaggtg actagccgtt aataaaaaga taaaaacaaa
    11941 aaataaaatc agccaataca ttgtttccca ccttctaaaa accaaactta gacagtctgg
    12001 acttcgtttt ctccacgact ttgttcatct gacagcatct ttttatcata agcttttaca
    12061 aatggataat agataataaa tgctatcaat gtgttgatca tgatcaagat aacaacacgc
    12121 cagtcatttc ctgctgaaag ataaccgccg attggagctg gtaatgtcca tgttggcaaa
    12181 acagtaaatc tattgaccat ttctaatttg actgcaaaat aactgataat agtcaaaact
    12241 aaaggaacta agttgaacgg gatgaacatg taaggattca acatcatagg agctccaaaa
    12301 attatgggct cattgatatt gaagatacca ggaataatag aaaaacgacc aacctctttc
    12361 aagaattttg atttacagaa gaatataaac aaaacagata ccacgatcgt tgctcctgca
    12421 ccaccaattg ttaccgtcca ttgataaaac tgttcaggag cgatataggg taattgggtg
    12481 gcagctgtac catctgccaa tgcctttgca ttctcatcca acatgatcag ccacattgga
    12541 cgaacaacag cccctacaat actcatgcca tggataccaa atgaccacaa caaatggatc
    12601 aaaaacattg gtagtaaaac accaagcaaa ttattcccta aaatattggt taaaggctcg
    12661 aataacgata ataaggcagc gtttacatca aagcccgcga cgcttcgaat cagccaagta
    12721 attgttaaaa tgactgcacc tggaatcaat gcctcaaaac tacgggcaac agcaggtggc
    12781 acttgttctg gcattttgat agtaatattt cgatttttaa agaaacgata tacttccact
    12841 gcaaaaatca tagataagat ccccgcaaac atcccagatg ctcccaaaga actcataggt
    12901 aagacccagc caagaggtgc ctcttcagga gttaaaacgt ttacaggaac ttgtaacata
    12961 aagaacgttc ctaaagataa tataccacct gtcacactat ctaatccata tgatttagct
    13021 agatccgaac ccataacaaa agttgcataa atagccatca atcccatcgt catcctaaag
    13081 ggtaaaacaa tcgttgccga ataaggggca ataaattcat tccatccagg aaacggtaaa
    13141 ttgataaaga ccataaaaaa tgtcccaatc aaaatcaatg acaatgttga aacaacccca
    13201 ttacgaattg ctaataaatg acgctgtgta ccaatttttg ccattggtgg gacaagtttc
    13261 tcttcgaccc atttcatcag cttttccact aaaaccactc cttttcaata aaatatgaac
    13321 cattcattgc atgacaacgc aaagaatgtt cataactaat tatataatta agaaatttta
    13381 tttcaatata ttctcttaaa taatcaaatt tatttttaag aatcctgaaa ataagttttc
    13441 taaatcatag gagcaacaaa aaaattc
//
LOCUS       DARBET010000067        13429 bp    DNA     linear   BCT 07-NOV-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4027
            SAMEA114569841-rid20112183.denovo.069, whole genome shotgun
            sequence.
ACCESSION   DARBET010000067 DARBET010000000
VERSION     DARBET010000067.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA114569841
            Sequence Read Archive: ERR12203666
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 13429)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13429)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001972618.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001972618.1
            Genome Coverage       :: 132x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/07/2023 13:18:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,041
            CDSs (total)                      :: 2,975
            Genes (coding)                    :: 2,908
            CDSs (with protein)               :: 2,908
            Genes (RNA)                       :: 66
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 67
            CDSs (without protein)            :: 67
            Pseudo Genes (ambiguous residues) :: 0 of 67
            Pseudo Genes (frameshifted)       :: 26 of 67
            Pseudo Genes (incomplete)         :: 28 of 67
            Pseudo Genes (internal stop)      :: 20 of 67
            Pseudo Genes (multiple problems)  :: 6 of 67
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13429
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA114569841-rid20112183.denovo.069"
                     /isolate="VRE4027"
                     /db_xref="taxon:1352"
                     /country="Denmark"
     gene            complement(<1..914)
                     /locus_tag="SCJ86_002184"
     CDS             complement(<1..914)
                     /locus_tag="SCJ86_002184"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_042964654.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine synthase family protein"
                     /protein_id="HEG1169711.1"
                     /translation="MIISTITEAIGSTPLYRFDNQHYSMPKGSAIYAKLEYLNPGGSI
                     KDRLGSYLIKEGFAQGKINEKTTIIEPTAGNTGIGIALAALTYKLRTVFVVPEKFSLE
                     KQQLMKALGAKIIHSPSEQGIIGAISKSKKLAEEISNSYLPLQFENKDNPAAYYHTLG
                     PEIFHEIKENIHSFVAGIGSGGTFAGTSTFLKEKYPDIRIIGVEPEGSVLNGGDPAPH
                     EIEGIGVEFIPPFLSPLSINQIETISDVEGFNYTRQLAREQGLLVGSSSGAAFAAALR
                     EIRRLPPGHRVVTIFPDAADRYLSKNIY"
     gene            1342..1905
                     /gene="efp"
                     /locus_tag="SCJ86_002185"
     CDS             1342..1905
                     /gene="efp"
                     /locus_tag="SCJ86_002185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010751911.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor P"
                     /protein_id="HEG1169712.1"
                     /translation="MISASDLRAGMTFVQDGKLIKVLDASHHKPGKGNTVMRMKLKDV
                     RSGATYDTTFRPEEKFEKAHIDTKPVQYLYTMDDTAFFMDLETYEQYEIPVETVEEEM
                     KYILENMEVKIQFFGSEVIGVQLPTTVVLRVVETQPSIKGATVTGSGKPATMETGLVV
                     NVPDFIETDELLEINTAEGSYVKRASK"
     gene            2006..2176
                     /locus_tag="SCJ86_002186"
     CDS             2006..2176
                     /locus_tag="SCJ86_002186"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301233.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEG1169713.1"
                     /translation="MKNKKVFDFIYILKDEWQKLEKEETALIYEEIEMIHKIENIRTI
                     PNYIEAKIFFHH"
     gene            2391..3650
                     /gene="lysA"
                     /locus_tag="SCJ86_002187"
     CDS             2391..3650
                     /gene="lysA"
                     /locus_tag="SCJ86_002187"
                     /EC_number="4.1.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301232.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diaminopimelate decarboxylase"
                     /protein_id="HEG1169714.1"
                     /translation="MTELIIGGVTASTLAEKYKTPLYVYDEEKMRQTMLSFKRGFVSK
                     RFDTKVLYASKAFQTIEMLHLVREYGFGLDVVSGGEIYTALQADFPRDTIYFHGNNKT
                     PEELIYALENDLLHIVADNLMEVELLAELSQRYLKKICIMLRLNVGVEAHTHEYIVTA
                     HIDSKFGMSYESKECQQALQLIKESSYLELEGFHAHIGSQIFDVTAWLAEIDKLTNYL
                     TDFDQPLSLNLGGGFGIRYTQNDQPIPVEEALKKLIEYTEEALEKRKLTIRQLLIEPG
                     RSIVGEAGTTLYTIGFIKKTPHKKYYFVDGGMTDNIRPALYQAEYDCDLAAKLSEEKT
                     EKVTIAGKMCESGDVVIKEAYLPKAESGDLLAVYSTGAYGYSMSSNYNRATRPAVVFV
                     NNGQSRLIVRRQAFSDLLRGEVSYDPS"
     gene            3637..4437
                     /gene="dapF"
                     /locus_tag="SCJ86_002188"
     CDS             3637..4437
                     /gene="dapF"
                     /locus_tag="SCJ86_002188"
                     /EC_number="5.1.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291395.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diaminopimelate epimerase"
                     /protein_id="HEG1169715.1"
                     /translation="MILHKYNGCGNDFILLDYAEDTDYSQLAASLCTKEKYDTDGLIA
                     VKIDPLEMIYYNKDGSRAPMCGNGIRCFARYVNDQNMMNEQEFNVETLAGTRKITVLD
                     PSPFYCSVDMGVPDDSPEKIKANQTYPIKNQEIEINGEKVTITSLFLGTIHTVVFVEN
                     AREEVWRKRGELICHHPLFKEKTNVNFVEVVNEQELIVRTYERGVGWTLACGTGCCAS
                     YVVAKETGKISADQVVVHLEQGDLMISGEDAIQMAGPAVHEWTRILEE"
     gene            4440..5321
                     /locus_tag="SCJ86_002189"
     CDS             4440..5321
                     /locus_tag="SCJ86_002189"
                     /EC_number="4.3.3.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002308109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-tetrahydrodipicolinate synthase"
                     /protein_id="HEG1169716.1"
                     /translation="MIKGSIVALITPMNEEGSVDYAGLEKLIQFHLDEQTDGLLVLGT
                     TGESSTLTQSEEEQILQLTVKKVAGRVPVIAGAGTNNTKETIEKAKHFASLGADALLV
                     ITPYYNKTSDAGLAAHFTAIAEASPIPLILYNVPSRTGMSIPIHVLVNLAEHPNIIGL
                     KEASGDMAYVMDAARLIGEEFFLYSGNDDLILPVMSVGGSGVISVWANIQPKIVHELV
                     KDTQDGRWQQAKEKQLNALELIHALFSETNPIPVKAAMSLLDLPSGPLRLPLVSLSKE
                     KKKQLAQLLLKERTVEK"
     gene            5318..6034
                     /locus_tag="SCJ86_002190"
     CDS             5318..6034
                     /locus_tag="SCJ86_002190"
                     /EC_number="1.17.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345386.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-tetrahydrodipicolinate reductase"
                     /protein_id="HEG1169717.1"
                     /translation="MKIGLIGSGQMGNRVEEVAQERGDTVLLLRTAPKEATTLAFTEL
                     PELLIDFSHPDNLEKILSYSLSYQLPLVIGTTGYTQSQFQEIKEHAKHVPVMYSANFS
                     FGIMVMNQLIKQAAAMLQGWQIELIEKHHSRKKDAPSGTANMLLQTIQEVQKLQPVYN
                     WQGKKREENQIGVHSIRAGSLPGEHDVLFAATDEIFTIKHESFSNRIFAAGAVEAADW
                     LKDQSPGYYKLEDLLMDKGV"
     gene            6040..6735
                     /gene="dapD"
                     /locus_tag="SCJ86_002191"
     CDS             6040..6735
                     /gene="dapD"
                     /locus_tag="SCJ86_002191"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338548.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2,3,4,5-tetrahydropyridine-2,6-dicarboxylate
                     N-acetyltransferase"
                     /protein_id="HEG1169718.1"
                     /translation="MEAQEIIRYIQTASKKTPVKVYLNVTEPIEFKESKVFGEGSSFV
                     VFGDYETIAPVLEAESEKIIEIEIETAARYSAVPLLDIKKINARIEPGAIIRDQVSIG
                     NNAVIMMGAIINIGAVIGENTMIDMGAVLGGRATVGKNCHIGAGAVLAGVIEPASAKP
                     VIVEDGVLVGANAVIVEGVHIGKDAVVAAGAVVLEDVAAETVVGGIPARVLKISDDQT
                     KENTALIAALREL"
     gene            6751..7752
                     /locus_tag="SCJ86_002192"
     CDS             6751..7752
                     /locus_tag="SCJ86_002192"
                     /EC_number="1.2.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349303.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate-semialdehyde dehydrogenase"
                     /protein_id="HEG1169719.1"
                     /translation="MREIDTCIVGATGLVGQTMLKVLEEYDFPVGRLKLLASKRSAGK
                     VCVFKKKKHTVQELTETSFEGYDLALFSAGASVSKEFAPIARSQGLFVIDNSSAWRED
                     SDIPLIVPEVNLEDYTLNRLIANPNCSTIQAVLPLKALQEAFGIQRVNYSTYQAVSGS
                     GQKGIEDLHRTQNDEEPVLYPYNISQTVIPEIDQPLENGYTKEEQKMINETRKILHQP
                     ALPVSATCVRVPIENGHGVSVAVQLQKEFTTEEIRECLSQFPGIQLEDDLAAHKYPTS
                     ILARGTDMVYVGRIRKDTSLENGLLFYTTADNIRKGAAANAVQIAAALMQKIAEVAR"
     gene            7749..9110
                     /locus_tag="SCJ86_002193"
     CDS             7749..9110
                     /locus_tag="SCJ86_002193"
                     /EC_number="2.7.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate kinase"
                     /protein_id="HEG1169720.1"
                     /translation="MSNTLKVAKFGGTSMSHEEQFKKVRTIVLSDPNRQVIVVSALGK
                     RTSDDDKVTDLLYLIYAYLQHHVDWTPLWKRICDRFIQVRDVLALQLPIEDELARIQK
                     QLEDEKITEDYLVSRGEYLTAQLMAEYLGYQFIDAKDLIFFDYDGKIDQEKTAQEITK
                     AFKRYPKMVVPGFYGANPAGKEKLLGRGGSDITGAILAKMLQAESYENWTDVSGIMMA
                     DPRIIDHPKIIEEISFRELREMAYMGANVLHEAAVFPVQEADIPIQIKNTNDPFAQGT
                     KISNHEIEKENGLTGIAGRKDFLSITLFKRHMSDEIGFIWKAMSIFAKHEISIEHIPS
                     GIDNIGVIVSAEAIADKLFLITKELKETLGVEEIVVIEDLALISVVGGPHKELIGLSG
                     KVLSILNKLEIRTSILSQGAQELNLIIGVPNNQYETVVKGIYEGMVNTDAERISNGTP
                     SNS"
     gene            9070..10221
                     /locus_tag="SCJ86_002194"
     CDS             9070..10221
                     /locus_tag="SCJ86_002194"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002308115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase"
                     /protein_id="HEG1169721.1"
                     /translation="MLKEYRMALHQIPEIGFHEFKTKKYLYEQVKNSGAVIHEIGETG
                     LLLYFDNQQEETIAFRTDIDALPVKEETNLSFASKHEGYMHACGHDGHMAMLLGLTDF
                     ITKHIEDLIYNVVLIYQPSEEISGGAQSVIQSGLLDKYEVQAIFGFHLWPGLAKGEVF
                     SRPGPLMAQSSETDIIIKGQSAHIASSEKGIDSLEAAVRFLKNVYDFDRQLPAEWNHL
                     MKFGQIQGGTIRNVLAGEVIVSGSIRSYSSDMQNYLKRGLDHLAEQFHSTATISFRYN
                     DGYPAVINHRGLYAALLDSNKLHELDAPVLQAEDFGIYTQQYPCVFFFLGVGDTPALH
                     DPTFDFDMDVLENGVEWYKTILFTENLLVKIDSSSKEERVNNDEFIQSI"
     gene            10196..11365
                     /locus_tag="SCJ86_002195"
     CDS             10196..11365
                     /locus_tag="SCJ86_002195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337895.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal phosphate-dependent aminotransferase"
                     /protein_id="HEG1169722.1"
                     /translation="MMNLSNRFSPRLSRIEVSKIRQFDQQISSIPDVIKLTLGEPDFP
                     TPEHVKQAGIAAIEEDFSHYTGMRGLEELREAACIFQQQRYGLTYDPQTEVLTTVGAT
                     EAIASALLSVLEEGDKVLIPAPAYSGYQPLVELAGAELIPIDTSDTGFVCQPEQFERA
                     FEQYGSAVKAVILNYPNNPTGTTLSAKQLKAIAEVLKKYPVFVISDEVYAELTYSGTH
                     MSIATYLPEQTIVISGLSKSHAMTGWRVGFIFAQKALIDELIKVHQYLVTAATTMSQK
                     AAAEALINGVKDSENMKEQYQTRRDYLVQQLAPLGFEVTQPNGAFYLFCKLPAAIQTD
                     SWQFCVELAEQAKVACIPGIAFGPEGEGYIRISYASSMEKLHEACKRIKDFLTEQ"
     gene            11540..12919
                     /locus_tag="SCJ86_002196"
     CDS             11540..12919
                     /locus_tag="SCJ86_002196"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336057.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HEG1169723.1"
                     /translation="MEKIKVVIVGASHGGHQSILELLTRYGENVDITLFEAGDYISFM
                     SCGMELYLEDKVTDVNDVRNFRPENFPQSNVHILNNHEVKTIDADKKTVTVTSASDNE
                     TKEYPYDKLILSSGVKPNNLPVPGADLENVYLMRGYNWATKIKEKLNDPAIKKIAVVG
                     AGYIGIEAAEASRKAGKEVVLLDVIDRPLGTYLDAEMTDILEQHLNENGIEVLTNAKI
                     EAFTGNGKVEAIKTSEKEIPADLVIQAAGVKANTEWLKGIVDLDERGWIQTNEYLQTN
                     LPDVYAVGDATLAYSIPARKKMPIALATVARREARYVVKHLFEEVPNKPFGGVVGSSA
                     LSVFDYHFAASGLNSFTAAKAGVDVRVSYYEDTLRPKYVPEEQGNPKVSIQLFSDPMT
                     HQLLGGAVLSTYDVTGQGNVLALAIQNKLTLEELAEADFFFQPGFDRQWSMLNLAAQQ
                     ALGEEPFVE"
     gene            13200..13429
                     /locus_tag="SCJ86_002197"
                     /pseudo
     CDS             13200..13429
                     /locus_tag="SCJ86_002197"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611166.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
BASE COUNT         4482 a         2340 c         2926 g         3681 t
ORIGIN      
        1 aagtaaatgt tttttgataa atagcggtct gctgcgtctg gaaaaatcgt aacgacacga
       61 tgaccaggtg gtaaacgacg aatttctctt aatgctgctg caaaagccgc accactcgaa
      121 cttccaacta atagtccttg ttctcgtgct aactgcctag tatagttaaa cccttctaca
      181 tcagagattg tctcgatctg attgattgaa aggggagaaa gaaacggcgg aatgaattct
      241 acaccgatac cctcaatttc gtgaggggct ggatcaccac catttaacac agagccctct
      301 ggttctacac cgattattcg aatgtcggga tatttttctt tcaaaaaggt agaagttcca
      361 gcaaaggttc caccactccc aattcctgct acaaaactat ggatattttc tttgatttca
      421 tgaaagattt ctggccctaa cgtatgatag taagcagcag gattatcttt attttcaaat
      481 tgaagaggca gatagctgtt tgatatctcc tctgcaagtt ttttactttt gctgatggca
      541 ccgatgattc cttgctcact aggagaatgg atgatctttg ctcccaatgc tttcatcaat
      601 tgttgttttt ctaagctaaa cttctctgga acaacaaaaa ccgttcttaa tttgtatgtg
      661 agtgcggcca gagcgatccc aattcctgta tttccggcag ttggttcgat gatcgtcgtt
      721 ttttcattga tttttccttg ggcaaatcct tctttgatca aataactgcc caatctatct
      781 ttgatacttc ctccaggatt taaatattct agttttgcat aaatagcaga acctttaggc
      841 atagaatagt gctgattgtc aaagcgataa agcggtgtgg aaccaatagc ttctgtaatt
      901 gtcgagataa tcatgtgggc ctcctaaaaa aagataataa aaaaaggagc tgactattaa
      961 atcagctcct tgaaagacat agagaaaaat tgagtagata aaaagataag ttacctggta
     1021 ctcattctat gaagggcaat taatagacat gacaaatagt atgctgcttt tatgcaccat
     1081 acaacagcaa cgattcaatt gtttttggtt gctgactgtc atgtgtatcc ctccaagtaa
     1141 aatttttatt aacgatagca tgaaaaaaaa taaaagtcaa aggcttgaaa gaaaaaaata
     1201 tttcgatagc atcgtttacc cgtgtttatt tttctgacaa ataggcatga gaccttaaaa
     1261 gatagagaat actctagttt tctggccggt tgtctgatac aataaagttg attatgataa
     1321 agaaaagaag gagcaaaaat aatgatctca gcaagtgatt taagagccgg tatgactttc
     1381 gtacaagacg gcaaactgat taaagttctg gatgcaagcc accataaacc tggtaaagga
     1441 aatacagtta tgcgtatgaa actgaaagat gtccgttcag gtgcaacata cgacacaacg
     1501 ttcagaccag aagaaaaatt tgaaaaagca catatcgaca caaaacctgt acaatatttg
     1561 tacacgatgg atgatacagc tttcttcatg gatttggaaa catatgagca atatgaaatt
     1621 cctgtagaaa cagtagaaga agaaatgaaa tatatcctag aaaacatgga agtaaaaatc
     1681 caatttttcg gtagcgaagt catcggcgtt caattaccaa caactgtcgt attacgtgtt
     1741 gtggaaacac aaccttccat caaaggtgca acagtcactg gttcaggtaa gccagcgaca
     1801 atggaaactg gcttagtagt caatgttcct gacttcatcg aaacagatga attgttggaa
     1861 atcaatacag cagaaggttc ttatgtaaaa cgtgcgtcaa aataattcgc taacggattg
     1921 ataagaattt gaaaacagag cttgacctct tgaaaggcag atcatcggat ctgcctttca
     1981 agaggttttt tgtggaatca gcaaattgaa aaataaaaaa gtatttgatt ttatttatat
     2041 tttgaaagat gaatggcaaa agctagagaa agaagaaact gcacttattt atgaagaaat
     2101 tgaaatgatt cataaaattg aaaatatccg aactattccg aactatattg aagcgaaaat
     2161 attttttcat cactaaatag tgttgaattt atgtttttat gtataaatag cttctaatag
     2221 ttcgggtatt ttgtttttat aaccattttt attcttattt ttaaaaaaat atttcgaaat
     2281 atactagaca attcagaaaa ttttgtttaa tattattcca ataaaattca aatctttatc
     2341 aaatgaaatt ccaataaaaa cggaaaaaaa gtgaaaagag gaatgaaaaa atgacagaat
     2401 tgataattgg cggagtcaca gcaagcacat tggcagaaaa gtataagaca cctctgtatg
     2461 tgtatgatga agaaaagatg agacaaacga tgctgtcatt caagcgaggc tttgtatcga
     2521 aacgttttga tacaaaagtg ctttacgcct ctaaagcatt ccaaacgatt gagatgctgc
     2581 atttagttcg tgaatatgga ttcgggctag atgttgtcag tggtggggaa atctataccg
     2641 ctttgcaagc tgattttcct agagatacta tctacttcca tggtaacaac aagacgccag
     2701 aggaactgat ctatgcactt gaaaatgatt tgttgcatat tgttgccgat aatttgatgg
     2761 aagttgaatt attagcagaa ttaagccaac gctatttaaa aaaaatctgt atcatgcttc
     2821 ggttgaatgt cggagtagaa gcacatactc atgaatatat cgtcactgcg catattgatt
     2881 caaaattcgg catgagctat gaaagtaaag agtgccaaca agcccttcag ttgataaaag
     2941 aaagcagcta tctagagtta gaaggatttc atgctcatat cgggtcacaa atttttgatg
     3001 ttactgcttg gttagctgaa atcgacaaat tgacgaatta cttaaccgat tttgaccaac
     3061 ctctttcctt gaatcttggc ggagggtttg ggattcgtta tacacaaaat gatcagccga
     3121 tcccagtcga agaggcacta aaaaaactga ttgaatatac ggaagaagct ttggaaaaac
     3181 gaaaactgac cattcgtcaa ttactgatcg aaccgggtag aagtattgtt ggagaagctg
     3241 gtacaacact atacacgatt ggtttcatca aaaagacacc acataaaaaa tattactttg
     3301 tagatggcgg gatgacagat aatatccgtc cagctctgta tcaggcagaa tacgattgtg
     3361 atttagcagc aaaactgtcg gaagaaaaaa cagagaaagt gacgattgca ggaaaaatgt
     3421 gtgaatcagg cgatgtggta attaaagaag cttatttgcc gaaagcagaa tcgggtgatc
     3481 ttttagctgt ttattccacg ggagcttatg gatactcgat gagcagcaac tacaatcgag
     3541 cgacacgacc agcagttgtt tttgtcaata atggccaatc aagactgatt gttcgcagac
     3601 aagcattttc tgatctacta aggggggaag tttcctatga tccttcataa atataatggc
     3661 tgcggaaatg attttatctt attagattac gcagaagaca cagattatag tcaattagcc
     3721 gcttctttat gcacaaaaga aaaatatgat acagacggat tgatcgccgt aaaaatcgat
     3781 ccgttggaaa tgatctatta caacaaagat ggcagccgcg cgccgatgtg cggaaatggc
     3841 attcgctgtt ttgctcgata cgtaaatgat cagaacatga tgaatgaaca ggaattcaac
     3901 gtagagacat tagcaggcac aagaaaaatt acggtgttgg atccctcacc tttttattgc
     3961 agcgtagata tgggagtgcc agatgattct ccggaaaaaa tcaaagctaa tcaaacttat
     4021 ccaataaaaa accaggaaat agagatcaat ggtgaaaaag taacgatcac tagtttattt
     4081 ttaggaacga ttcatacagt tgtttttgtt gaaaatgcgc gtgaggaagt ctggagaaaa
     4141 agaggtgaac tgatctgtca tcatcctttg ttcaaagaaa aaaccaatgt caactttgta
     4201 gaagtagtca atgaacaaga attgatcgtc cgtacctatg aaagaggagt aggttggaca
     4261 cttgcttgcg gtacaggttg ctgtgcctct tatgtggttg ctaaggaaac tgggaaaatt
     4321 tcagcagatc aggtagttgt tcacttggaa caaggagact tgatgatctc tggtgaggat
     4381 gcgatacaaa tggctggccc agcagttcat gaatggacaa gaatattgga ggaatgaaga
     4441 tgataaaagg ttcgatcgtg gcgctgatca cgccgatgaa tgaagaggga tcagtagatt
     4501 atgctggttt ggaaaaattg atccaattcc atttagatga acaaacagat ggattattag
     4561 ttttaggtac aacaggagaa tcttctacgc tcacacaatc tgaggaggag cagatccttc
     4621 aactgacagt aaagaaagtg gctggccgtg tgccggtgat tgcaggagct ggaacgaata
     4681 atacgaaaga aacgattgaa aaagcgaagc attttgcttc tttgggggca gacgcacttt
     4741 tggtcatcac cccttactac aacaaaacaa gtgatgcagg actagctgct cattttacag
     4801 caattgcaga agcctcaccg attccgctta ttttgtataa tgttccttct agaacaggca
     4861 tgtcgattcc aatccatgta ttagtaaatt tagcagaaca tcctaatatc attggtttga
     4921 aagaagcctc aggagatatg gcttatgtga tggatgccgc tcggttgatt ggagaagaat
     4981 tcttccttta cagtggaaat gatgatttga tcctaccagt aatgagtgta ggaggaagcg
     5041 gtgtaataag tgtatgggcg aatatccaac ctaaaatagt ccatgaatta gtaaaagata
     5101 cgcaggacgg aagatggcag caagcaaaag aaaagcaatt aaatgcactt gaactgatcc
     5161 atgccttgtt cagtgagacc aatcctattc ctgtcaaagc agccatgtca ttattagact
     5221 taccttccgg acctctacgc ctgcctttag tttctctttc aaaagagaaa aagaaacaat
     5281 tagctcagct tttgttaaaa gaaaggacgg ttgaaaaatg aaaatcggac tgattggcag
     5341 tggacagatg ggaaatagag tcgaagaagt tgcacaagaa agaggagata cggtccttct
     5401 tttacggacg gcacctaaag aagcaactac gcttgctttt acggaactgc cagaactgct
     5461 tattgatttt tctcatccag ataatttaga gaaaatcctt tcgtatagct tatcatatca
     5521 acttccgtta gtcattggca cgacaggtta tacacaatca cagttccaag aaataaaaga
     5581 acatgccaaa catgtacctg tgatgtatag tgctaatttt tcgttcggca tcatggtgat
     5641 gaatcaacta atcaagcaag cagctgcaat gttgcaaggc tggcagatcg aactgattga
     5701 aaaacatcat agtcgtaaaa aagatgcacc atctggtact gcaaatatgc tgcttcaaac
     5761 aatccaagaa gtgcaaaagc tgcagcccgt atacaactgg caagggaaaa aaagagaaga
     5821 aaaccaaatt ggtgtacaca gtattcgtgc aggttcttta cctggtgaac atgatgtatt
     5881 gtttgctgct actgatgaaa tatttacgat caaacatgaa tctttttcta atcgaatctt
     5941 tgctgcaggc gcagtagaag cagcggactg gttgaaagat cagtcaccag gttattacaa
     6001 attagaagat ttattaatgg acaaaggggt gtaaaaaacg tggaagctca agaaatcatc
     6061 cgctacattc aaacggcatc taaaaagacg cctgtgaaag tctatctgaa tgtaaccgag
     6121 ccaatcgaat ttaaagaaag taaagttttt ggtgaaggaa gcagcttcgt tgtttttggt
     6181 gactatgaga cgattgctcc ggttttagaa gctgaatcag aaaaaatcat tgaaatcgaa
     6241 atcgaaactg ctgcaagata cagcgcagtt cctttgctag atattaaaaa aattaatgct
     6301 cgtatcgaac caggtgctat tatcagagat caagtgtcaa tcggcaacaa tgccgtgatc
     6361 atgatgggag cgattatcaa tattggtgca gtaatcggtg aaaacacgat gattgatatg
     6421 ggtgctgtct taggtggacg agcgacagtg ggcaaaaact gccatattgg tgcaggagct
     6481 gtcttagcag gtgtgatcga accagcatcg gccaaaccgg ttatcgtaga agacggcgta
     6541 ctggtaggtg cgaatgcagt gattgttgaa ggtgtccata tcggaaaaga tgcagtcgtg
     6601 gcagcaggag cagtggtttt agaagatgtg gcagcagaaa ctgttgtcgg tggaatacct
     6661 gcacgtgtat taaaaataag tgatgatcag acaaaagaaa atacagcatt gattgctgcg
     6721 ttacgcgaat tatagaggag atggagaaaa atgcgcgaaa tagatacctg tattgtgggg
     6781 gcaaccggat tagtcggcca aacgatgctc aaagtgttag aagaatatga ttttccagta
     6841 ggacgattga aattattagc ttcaaaacgt tcagccggta aagtttgtgt attcaagaaa
     6901 aagaaacaca ctgtacagga attgacagaa acttcatttg aaggatatga tttagcgcta
     6961 ttttctgcag gtgcttctgt atccaaagag tttgcgccaa tcgcacgaag tcagggacta
     7021 tttgtcatag ataattcctc agcatggcgg gaagattcgg atattccatt aatcgttcca
     7081 gaggtcaatc tggaagacta tacattgaat cggctgattg caaatccaaa ttgttcaacc
     7141 attcaagcgg tacttccgct taaagcttta caagaagcct ttggtatcca gcgagtcaat
     7201 tattcgacct atcaagcagt ttctggttcc gggcaaaaag gcattgaaga tcttcatcgt
     7261 acgcaaaatg acgaggaacc agttttgtat ccttataata ttagccaaac ggtgattccg
     7321 gaaatcgatc agcctttgga aaatgggtat acaaaagaag aacagaaaat gataaatgag
     7381 acccgtaaga ttctgcatca gcctgctttg ccagtatcag caacctgcgt acgtgtgcca
     7441 atcgaaaatg gacatggagt ctctgtggca gttcaactgc aaaaagagtt tacgacggaa
     7501 gaaattagag agtgtctcag ccagtttcca gggattcaat tagaagatga tctagcagca
     7561 cataaatatc cgacgagcat tcttgctaga ggaacagata tggtttatgt tgggaggatc
     7621 agaaaagaca cttctttaga gaacggattg ttgttctata cgactgccga taacatacgc
     7681 aaaggagccg ctgcgaatgc cgtacagatt gctgcagctt taatgcagaa aatagcggag
     7741 gtggcaagat gagtaatacg ctgaaagtag cgaaattcgg cggaacatcg atgtctcatg
     7801 aagagcaatt caaaaaagtc cgtacaattg ttctttcaga tcccaatcgc caagtgatcg
     7861 ttgtttctgc tttaggcaaa cgtacgtcag atgatgacaa agtaacagac ttactttatt
     7921 tgatttatgc ttaccttcag catcatgttg attggacacc cctttggaaa aggatttgtg
     7981 atcgtttcat tcaagtaaga gatgttcttg ccctgcagtt acctattgaa gatgaactag
     8041 cacgcataca gaaacaactg gaagacgaaa agataactga agattattta gtaagtagag
     8101 gtgaatatct gactgctcaa ctgatggctg agtatcttgg ttaccagttt attgacgcaa
     8161 aagacctgat tttctttgac tatgatggga aaatcgatca ggaaaaaaca gcacaggaaa
     8221 tcacaaaagc atttaaaaga tatccgaaaa tggttgttcc agggttttac ggggcgaacc
     8281 ctgcaggaaa agaaaaatta ttgggccgtg ggggaagcga cattaccggc gcgatactgg
     8341 cgaagatgtt gcaagcggaa agttatgaga attggacgga tgtttcagga atcatgatgg
     8401 ctgatccgcg aatcatcgat catcctaaaa tcatcgagga aatctctttt agagaactga
     8461 gagagatggc ttatatgggg gctaacgttc ttcatgaagc agctgttttc ccagttcaag
     8521 aggctgatat ccccatccaa ataaaaaata ccaatgatcc tttcgctcaa gggacaaaaa
     8581 tatcgaatca cgaaatagaa aaggaaaatg gactgacagg aatagctgga cgcaaggatt
     8641 ttctttccat cactttattc aaacgtcata tgtccgatga aatcgggttt atctggaaag
     8701 caatgagcat ttttgctaaa catgagatat caatcgaaca tattccttca gggatcgaca
     8761 atatcggagt gatcgtctca gcagaagcga ttgccgataa gttatttctc atcacgaaag
     8821 aattaaaaga aacacttggc gtggaagaaa tcgtcgtgat cgaagactta gctttgatct
     8881 ctgtcgttgg gggtccgcat aaagaattga ttggattatc tgggaaagtg ttatcgatat
     8941 tgaacaaatt agaaattcgc acatcgatct tatcacaagg agcacaggag ttgaatctga
     9001 tcatcggtgt accgaataat caatatgaaa cagtagtgaa gggtatctat gaagggatgg
     9061 tgaatacgga tgctgaaaga atatcgaatg gcactccatc aaattcctga aatcgggttt
     9121 catgaattca agacaaaaaa atatttatat gaacaagtaa aaaacagcgg ggccgttatc
     9181 catgaaatcg gcgaaacagg actactcctt tattttgata atcagcaaga agaaacgatt
     9241 gcttttcgaa cagatatcga tgcactgcca gtcaaagaag aaacgaatct ctcttttgct
     9301 tctaagcatg aaggttatat gcatgcatgt ggtcatgacg gacatatggc gatgctttta
     9361 gggttgactg actttataac taaacacatt gaggatctta tttataatgt agtactgatc
     9421 tatcagccat cagaagaaat ttccggtggc gcacaaagcg ttattcagtc aggattgttg
     9481 gataaatatg aagtacaagc aatatttggc tttcatctat ggcctggact agctaaggga
     9541 gaagttttct ctcgaccagg acctttgatg gcacaaagca gtgaaacaga cattatcatt
     9601 aaaggccagt ctgcacatat tgcttctagt gaaaaaggaa tcgatagtct ggaggcagct
     9661 gtacgatttt tgaagaatgt ttatgatttt gaccggcaat taccggcaga gtggaatcat
     9721 ctaatgaaat ttggccaaat ccaaggtgga acgatccgca atgttttagc tggagaagtt
     9781 attgtatccg gaagtatccg ttcttacagt tcagatatgc aaaattattt gaagagggga
     9841 ttagaccatt tagcagaaca gtttcactct acagctacta tttcttttcg ctataatgac
     9901 ggctatccag ctgtaatcaa ccatagaggc ttatatgcag cactgttaga ttcaaacaaa
     9961 ctacatgaat tagatgctcc agttcttcaa gccgaagatt ttggcatata tacgcaacaa
    10021 tatccatgtg tatttttctt tttaggtgtc ggtgataccc ctgctttaca tgatccgacg
    10081 ttcgattttg atatggatgt tttagaaaat ggtgtagaat ggtacaaaac gattcttttt
    10141 acagaaaatc ttcttgtaaa aatcgatagc agttcaaaag aggagcgagt aaataatgat
    10201 gaatttatcc aatcgattta gtcctaggct ttctagaata gaagtatcaa aaatacgaca
    10261 attcgatcag caaatatcgt cgataccaga tgtgatcaaa ctcaccctgg gagaaccgga
    10321 ttttccaact cctgaacatg tcaagcaagc aggaatagca gcgattgaag aagacttttc
    10381 tcactatacc ggaatgagag gattagagga actgcgggaa gcggcttgta tatttcagca
    10441 acagcgttat ggattaactt atgatccgca aacggaagtt ttgacgacag ttggtgcaac
    10501 ggaagcgatt gcctctgcgt tgttatctgt tttggaagaa ggagacaagg ttcttattcc
    10561 agcgcctgct tattcaggat atcagccatt agtggaacta gctggtgctg aattgatccc
    10621 gatagatact tctgacacag ggtttgtctg tcagccggaa caatttgaac gagcatttga
    10681 acaatatgga tcagctgtca aagcagtcat tttaaactat cctaacaatc ctactgggac
    10741 gacattgtct gcaaaacaat tgaaagcaat cgctgaggtt ttaaaaaaat atccggtatt
    10801 tgttataagt gatgaagtat atgctgaatt gacttatagt ggaacgcata tgtccatagc
    10861 tacttatctt ccagaacaaa caatcgtgat aagcgggtta tccaagtcac atgcgatgac
    10921 tggttggcga gtgggcttta tttttgctca aaaagccctt attgacgaac taattaaagt
    10981 ccatcaatat ctggtgactg ccgctacaac gatgtcgcaa aaagctgcgg ctgaagcgct
    11041 aataaatggg gtgaaagata gcgagaatat gaaagagcaa tatcaaacac gaagagacta
    11101 tctggtccag caattggcgc ctttaggatt tgaagtgacc cagccaaacg gtgcatttta
    11161 cttgttttgc aaactcccag ctgccattca aactgactct tggcagtttt gtgtagaatt
    11221 agcggaacag gcaaaagttg cgtgtatccc aggaattgca tttggcccag aaggagaagg
    11281 ttatatccgg atttcttatg ctagcagcat ggaaaaatta catgaggcct gcaaacggat
    11341 caaagacttt ttaacagaac agtgattcat agattttatc aaaaaaacaa tcgttaattt
    11401 tgcggtatat tctgcaagga gacgattgtt ttttaaattt agttcaaatg aacgcgtttc
    11461 ttttgattca taactaatgt aatgttaagt ttaaattgtt tagaatgatt ttaaaagaat
    11521 attcaaggag gtttcatcaa tggaaaaaat caaagttgtc attgtaggtg catctcatgg
    11581 agggcatcaa tcgatcttgg aactattgac acgatacggt gaaaatgtag atatcacgct
    11641 atttgaagca ggagactata tttccttcat gtcatgtggt atggagctgt atttagaaga
    11701 caaggtaaca gatgtaaatg atgtacgtaa tttcagacca gaaaactttc cgcaatccaa
    11761 tgttcatatt ttaaataatc acgaagtaaa aacgatcgat gccgataaaa aaacagtaac
    11821 ggttactagc gcatcagata acgaaacaaa agaatatcct tatgacaaat tgattttgag
    11881 ttctggtgtc aaaccaaaca atctgccagt accaggagca gatctagaaa atgtctactt
    11941 gatgcgtgga tacaattggg caaccaaaat caaagaaaaa ttaaatgatc cggctattaa
    12001 aaaaatcgct gtcgtaggtg cgggttacat tgggatcgaa gcagcagaag caagccgaaa
    12061 agctgggaaa gaagtcgtat tgcttgatgt gatcgataga cctttaggca cttatttaga
    12121 tgctgagatg acagatattt tagaacaaca cctaaatgaa aacggtattg aagttttgac
    12181 aaatgcaaaa atcgaagctt ttactggtaa cggaaaagtt gaggcaatca aaacaagtga
    12241 aaaagaaatc ccagcagatc tagtcattca agctgccggt gtgaaggcaa atacagaatg
    12301 gttaaaaggg atcgtcgact tagatgaacg tggctggatc caaacaaatg aatacttgca
    12361 aacgaatctt ccagatgtgt atgcagtagg agatgctaca cttgcttatt ctatcccagc
    12421 acgcaaaaaa atgccgatcg ctttagcgac tgttgcacgt agagaagcac gttatgtagt
    12481 aaaacatctt tttgaagaag taccaaacaa accgttcggc ggagtcgtag gttcctctgc
    12541 attaagtgtc tttgactatc attttgcagc aagcggattg aatagcttta cagcagcaaa
    12601 agcaggagta gatgtacgag tgtcttatta tgaagatacg ttacgaccaa aatatgtacc
    12661 agaagaacaa ggaaatccaa aagtgtccat ccaattattc tcggatccaa tgacccatca
    12721 attgctagga ggagcagtat tatctaccta tgatgttaca ggtcaaggaa atgtattggc
    12781 actagcgatc caaaacaaac tgactttaga agaactagca gaagctgact tcttcttcca
    12841 gccaggcttt gaccgacaat ggagcatgtt gaatctagcc gctcaacaag cgttaggaga
    12901 agaaccattt gtagaataag aaaacgaaat atttgaaaag aggttgtgaa aaagctgcct
    12961 tgcatcaagt aataagaaca gccaagtaaa aacagcttct tatgttttta acttggctgg
    13021 acttattacc gcagatgcaa gcttttgaac accgtttatt ttcaaaaaag ggactgtgac
    13081 acgatttgtg taccgacccc caaaagttag accaaaaaat ctaacttttg gaggtcggta
    13141 ttttttatgg ttaaatatac ttttgaactt aaattaaaga tcgttcatga ttatttggat
    13201 ggcaaaggcg attggaccgt aaaaatccag atcaagagat tgaagcggaa atgcttaaaa
    13261 ttcgggagaa gcacaaggat tatggctgtt tacggatgac caatgaacta agaaaccgcg
    13321 gtttctctat caataaaaag aaagttcagc gcttgatcaa gaagctagga ataaaagtaa
    13381 cggcatacac gcgtaaatca cgtcgctaca attcttatcg aggaaaagt
//
LOCUS       DARBET010000068        13404 bp    DNA     linear   BCT 07-NOV-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4027
            SAMEA114569841-rid20112183.denovo.070, whole genome shotgun
            sequence.
ACCESSION   DARBET010000068 DARBET010000000
VERSION     DARBET010000068.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA114569841
            Sequence Read Archive: ERR12203666
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 13404)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13404)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001972618.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001972618.1
            Genome Coverage       :: 132x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/07/2023 13:18:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,041
            CDSs (total)                      :: 2,975
            Genes (coding)                    :: 2,908
            CDSs (with protein)               :: 2,908
            Genes (RNA)                       :: 66
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 67
            CDSs (without protein)            :: 67
            Pseudo Genes (ambiguous residues) :: 0 of 67
            Pseudo Genes (frameshifted)       :: 26 of 67
            Pseudo Genes (incomplete)         :: 28 of 67
            Pseudo Genes (internal stop)      :: 20 of 67
            Pseudo Genes (multiple problems)  :: 6 of 67
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13404
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA114569841-rid20112183.denovo.070"
                     /isolate="VRE4027"
                     /db_xref="taxon:1352"
                     /country="Denmark"
     gene            79..1125
                     /locus_tag="SCJ86_002198"
     CDS             79..1125
                     /locus_tag="SCJ86_002198"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349488.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan DD-metalloendopeptidase family
                     protein"
                     /protein_id="HEG1169724.1"
                     /translation="MTKPNHIESSKKEAPKTEGQTKTFSTPQQIESTESFQSQPILSG
                     NEEIHFDKNESVENFIMKIGESARKIGQKYDLYASVMIAQAILESASGQSQLAQAPNY
                     NLFGIKGTYNGNFVIMVTNEDLGNGTLYTTQSKFRVYENYEESFEDYAKLLTKGISGN
                     KDFYAGALKANSKTYREATKFLTGRYATDTQYYLKLNELIKTYDLTNYDKEVKGPQMS
                     KTGYQVPIHNYSITSLFGNRGNEFHRGLDMAATQGEPIHAVKAGKVVKAEFHYSWGNY
                     VVIQHEDGSTALYAHQQQYLVKTGDNVLQGQIIGYVGSTGNSTGPHLHLEICKDSSLS
                     QSMLVDPKTLLFGD"
     gene            1474..2106
                     /locus_tag="SCJ86_002199"
     CDS             1474..2106
                     /locus_tag="SCJ86_002199"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297340.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AP2 domain-containing protein"
                     /protein_id="HEG1169725.1"
                     /translation="MKERYGHLTVVEEITHNGYKKYRCLCDCGNETIVYRSNLLSGRT
                     KSCGCGNYKNRHKYKNLTNKTFGKLVAIKPTKLRSEGTVVWECQCLCGRKKLVSAKRL
                     LKGDVKSCGCLKSKKHHIENKKFGLLTAKYPLDNNCSWKTKWVCHCECGSMCEISYSN
                     LFFGHTQSCGCLKRQETRTCVEGTVIECLTSKLPKNNTSGVKGVYRSKEK"
     gene            2694..4133
                     /locus_tag="SCJ86_002200"
     CDS             2694..4133
                     /locus_tag="SCJ86_002200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297342.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEG1169726.1"
                     /translation="MYSYQEDAKVQKNRWWILIAISMFTFMSTLDGSIVNIALPTISD
                     DMGIPMNQAEWVVSVYLMIVCSCLLLFGKIGDSRGKIKVFRIGTLIFVLGSLLCGFNH
                     SLGILLFARVVQAIGASMTMATNAGIITEVFPIHERGRALGAIGAFVSLGSIAGPGLG
                     GLILSQFSWAYIFWINVPVGIVTMLIAEKFLPKDITMSGHTIDGGGFAAIAATIISFF
                     GAIFLGQESGFFHPVPLVLFVISVIAFVSFLKVEKKAASPLISFGIFKNKIFTMSLIT
                     ALLIFSSNFFVNVVIPFYLQNARGLSPSYAGLLMMVFPLMMVVGAPLSGYLTDRVGPE
                     ILVLVGLGLLSVTQVMYMFLSVDSPLWLYIVATAIMGAGNALFQSPNNTMVMSSVTIE
                     NLGLAGSMNSFARNLGMVSGISLSTTVLYQAMSAKMGQQVTNYIANRPDVFLYGMKIT
                     FLGSFVLCFVAFFLTIWRIKKQGGIQKNV"
     gene            4428..4646
                     /locus_tag="SCJ86_002201"
                     /pseudo
     CDS             4428..4646
                     /locus_tag="SCJ86_002201"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016251366.1"
                     /note="internal stop; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            4895..5110
                     /locus_tag="SCJ86_002202"
     CDS             4895..5110
                     /locus_tag="SCJ86_002202"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297343.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEG1169727.1"
                     /translation="MNAGIADMNLIKKTLNDFTSNSISKGTGINLSTIKKLKSGERSV
                     EKLNLLDAIKITEFAMKNGKAEIEIWR"
     gene            complement(5327..5583)
                     /locus_tag="SCJ86_002203"
                     /pseudo
     CDS             complement(5327..5583)
                     /locus_tag="SCJ86_002203"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287522.1"
                     /note="frameshifted; internal stop; incomplete; partial in
                     the middle of a contig; missing C-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            complement(5770..6681)
                     /locus_tag="SCJ86_002204"
     CDS             complement(5770..6681)
                     /locus_tag="SCJ86_002204"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297345.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein"
                     /protein_id="HEG1169728.1"
                     /translation="MKIKIERNQKEKSPKKKKERVVSVGKHRKMVLALWLLLSCSLAF
                     GVYKNFTAIDQHTTHEKVVIKEKVVNTSGVESFTEDFAKEYFSWKNNKEAIEKRMSNL
                     EQYLTENGLALIQDMVRADIPTSSEVQSVKILDVEKHSEEFVVSFLVEQHITEGKKTQ
                     SISSAYRVTIFEDENRNHLVTSLPTMIGKPDKAKYEAKQVENDSEIDAKTTEEITDFL
                     ETFFKLYPSASEKELEYYVENGAMRPINIDLKFSELENPVYLQNGKNFLVSTKIKYLD
                     NVSKTTSYFQYKLVLQKNGNWKIVDVK"
     gene            complement(6700..7704)
                     /locus_tag="SCJ86_002205"
     CDS             complement(6700..7704)
                     /locus_tag="SCJ86_002205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346909.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional lysozyme/C40 family peptidase"
                     /protein_id="HEG1169729.1"
                     /translation="MNLKKVGVISLTFLTISFMGILMCVGLLFGEDSEGNGSSGTSPG
                     GTSVSEEVLKHRAMVEKYAKESDITEYIPILLAIIQVESGGTMEDVMQSSESAGLPPN
                     SLSTEASIKQGCLYFASLVKRSKELGCDQDSIIQAYNYGGGYLDHVGKNGKKHSFALA
                     ESFSKEKSGGQKVDYPNPIAIKENGGWRYNYGNMFYCLLVKQYLTTTQFDDKIVQEIF
                     DEAFKYEGTAYVFGGSSPETGFDCSGLTQWCYAKVDLKLPRVAQDQYDAMTHIDLKDA
                     KPGDLVFFHSTYDAGTYVTHVGIYAGENRMFHAGDPVGWTNLTENYWRQHLIGAGRYK
                     "
     gene            complement(7701..9824)
                     /locus_tag="SCJ86_002206"
     CDS             complement(7701..9824)
                     /locus_tag="SCJ86_002206"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002407825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FUSC family protein"
                     /protein_id="HEG1169730.1"
                     /translation="MKKKLFRIVGIVFISILTLIVLLSLVGTVAEATGLVDDTVEAGN
                     LYSKYSLNNYQLDFFVDSSWDWLPWNWGDGLGKSVMYGLYAITNFIWTVSLYLSNATG
                     YVVQEAYKLDFISDTAESIGKNIQTLAGITENGLQTSGFYFGFLLLMILALGVYVAYT
                     GLLKRETTKAVRAVINFVMIFLLSGSFIAYAPTYITKINDFSSDVSEAALTLGTEIVV
                     PNSESQGKDSVDLIRDSLFSIQVQQPWLLLQFDDSNIEEIGEDRVNKILSVSPDENKG
                     KDREEAVKAEIEDNDNANLSITKTMNRLGTVVFLVLFNIGISFFVFLLTGIMLFSQIL
                     FIIFAMFLPISFLLSMLPTYESLGKKAIIRLFNTIMMRAGVTLVITTAFSISTMFFNI
                     SATYPFFMVAFLQIVTFAGIYFKLGDIMSMFNLQSNDSQSMGRRVMRKPQMLMNRKLR
                     QLNRNVGRALAFGGGAAVGNKLAKEQSKSKFKPSGSSLRKNSRLPNDHEILSDLTKEN
                     PVSNNKKQSRMNLAGRKIGKVLDTQALVKDKTKQVKDQVRNTPTNLKYNLHKGIEKSK
                     KAPEEFKRGLFQEKADRVDLREKQRQRRNAKMDEKRKILSEATNRQGKRRANIPIDKA
                     AQPQKDMMLKKEAPKRIVRANLNPEIKRKLTSQEIISKGKSQSASKKNSFQQVKQRST
                     LPKQTNQKVQKRMNHRPKSRSGEKK"
     gene            complement(9829..12276)
                     /locus_tag="SCJ86_002207"
     CDS             complement(9829..12276)
                     /locus_tag="SCJ86_002207"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335929.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HEG1169731.1"
                     /translation="MYPIKYIENNLVFNQEGECFAYYELIPYNYSFLSPEQKYQVHDN
                     FRQLIAQNREGKIHALQIATESSIRATQERSKKEITGRLTEVAKQRIDLQTEALVSMI
                     GDSQIDYRFFIGFKLIATDEEVNLKNLKKSFFSGFQEFVYGVNHHLMGDFVSLSNEEI
                     RRYTKLEKLMESKLARRFKVRRVTPSDLMYLIEHIYGEKGTPFEEYEFQLPKKKLKSE
                     TLVKRYDLLRPSRCLIEEKPRYLCMEHENHESYVAYLTINTIVGEMEFPSSELFYYQQ
                     QQFTFPIDTSMNVEIVTNKKALATVRNKKKELKDLDNHAYQSDNETNSNVLDALDSVD
                     ELETTLDQSKESMYKLSYVVRVSAESVDELKRRCDEVLDFYDDTNVKLVRPFGDMMGL
                     HEEFLPSSKRYMNDYIQYVTSDFLAGLGFGATQMLGELEGIYFGYNVDTGRNVYLKPA
                     LASQGVKDSVTNALAAAFLGSLGGGKSFSNNLLVYYAVLFGGQAVIVDPKGERGGWKE
                     TLPEIADEINILNLTSKPKNQGLLDPYVIMEKKKDSESLAIDILTFLTGISSRDGEKF
                     PVLRKAIRTVTQSNQRGLLQVINELRKEGSPVAENIADHIESFTDYDFAHLLFSDGSI
                     TQSISLDRQLNIIQVADLVLPDAETTFEEYTTMELLSVSMLIVISTFALDFIHSNREI
                     FKIVDLDEAWSFLQVAQGKTLSNKLVRAGRAMNAGVYFVTQNSDDLLDERLKNNIGLK
                     FAFRSTDIHEIKKTLEFFGLDREDESNQKRLRDLENGQCLMQDLYGRVGVVQVHPVFE
                     ELFHAFDTRPPVQERME"
     gene            complement(12263..12652)
                     /locus_tag="SCJ86_002208"
     CDS             complement(12263..12652)
                     /locus_tag="SCJ86_002208"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335930.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein"
                     /protein_id="HEG1169732.1"
                     /translation="MKKIKSYTSIWAVEKVIYAINDFQLPFPVTFNQMAWFVLSLLFV
                     IVFAHVPPLSMIEGAFLKYLGIPVAVTWFMSQKTFDGKKPLGFLRSFISYHLRFKVTF
                     AGKKVKEQKKRWDEPITLVRSVNYVPD"
     gene            complement(12712..13215)
                     /locus_tag="SCJ86_002209"
     CDS             complement(12712..13215)
                     /locus_tag="SCJ86_002209"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010718210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antirestriction protein ArdA"
                     /protein_id="HEG1169733.1"
                     /translation="MEQMRVYIANLGKYNEGELVGAWFTPPVDFDEVKERIGLNDEYE
                     EYAIHDYELPFEIDEYTPIEEINRLCGLAEELEGTPVGEVASEIQHAFFNSFEEMVEH
                     VDDIIYYPDCDDMSDVAYFLIVEAGDFGEVPEHLKTYIDFDACGRDLEIGGNYLVTSR
                     GVFEYPY"
     gene            complement(13228..13389)
                     /locus_tag="SCJ86_002210"
     CDS             complement(13228..13389)
                     /locus_tag="SCJ86_002210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311671.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEG1169734.1"
                     /translation="MAIVVIGLFLGFKREFSKLIGFLVIALIAVGLVFNASGVKDVLL
                     SLFNRIVGA"
BASE COUNT         4099 a         2642 c         2454 g         4209 t
ORIGIN      
        1 tcaagtgaaa gtagtacgat ttcaagtacc gaatcttcaa caacttcaag tagtacaagt
       61 gaaataactt cttcgaaagt gacaaaacca aaccatatag aatcttcaaa aaaggaagcg
      121 ccaaaaacgg aaggacaaac aaaaactttt tcgacaccac agcaaataga atcaacagaa
      181 tctttccaat cacaaccaat actatctggt aatgaagaaa ttcattttga caaaaatgaa
      241 tctgtagaaa attttattat gaaaattggt gagtctgcga gaaaaattgg tcagaaatat
      301 gatttatatg cttctgttat gattgcacaa gcgatcttag aatctgccag tggtcaaagc
      361 cagttagctc aggcaccgaa ttacaattta tttgggataa aaggaactta taatggaaac
      421 tttgtcatta tggtaacaaa cgaagatttg ggtaatggaa cgctttatac gacccaatca
      481 aaatttagag tgtatgaaaa ctatgaggaa agctttgaag attatgccaa attattaact
      541 aagggaatta gtggaaataa agatttttat gcaggagcat taaaagctaa tagtaaaaca
      601 tatcgagaag caactaaatt ccttacagga cggtatgcaa cagatacaca atactatttg
      661 aagctaaatg aattaattaa aacctacgat ttaaccaact atgataaaga agttaaaggt
      721 ccacaaatga gtaagactgg ataccaagtg cctattcata attattcaat aaccagtttg
      781 tttggaaatc gtggaaatga atttcaccga ggacttgata tggcagctac acagggtgag
      841 ccgattcatg cagttaaagc tggtaaggtt gttaaagcag agtttcacta ctcttgggga
      901 aattacgtgg tgatacaaca cgaagatgga tcaaccgcgt tatatgctca ccagcaacaa
      961 tacctagtaa aaactggaga taatgttttg caaggacaaa taattggcta tgtaggaagt
     1021 actgggaata gtactggccc acaccttcat ttggaaattt gcaaagatag tagtctttct
     1081 cagagcatgt tagttgatcc gaaaacctta ttattcggag actaaaaaag gtaatcaatc
     1141 tgacaaagta acaagaaaat ggttggattc ttagaccttt gatcaggata ttgagactgg
     1201 aattgatatc tcgattacga tgggtttgac aaataaaaca agtccattat cgaatatcga
     1261 gtgtattatt gccgtcttta tgctcatatt ttgagcaggt ttcactagat gaaaacaatt
     1321 tatctatctt gaggatttcg tgtacgtatc gttcagtctt gtattgtaaa ttagtcataa
     1381 aatcagacta agaaaaattg gaaatatttt tttctgattt attaaaagtt aattgaaatt
     1441 atatggtact tctttcttgg gagggtaggt gcagtgaagg aaagatatgg tcaccttact
     1501 gttgttgagg agattactca taatggatat aaaaaatatc gttgcctatg tgactgtggg
     1561 aatgaaacga ttgtatatcg atcaaactta ctatcaggta gaaccaaaag ttgtggatgt
     1621 ggaaattata aaaataggca taaatataaa aatttgacaa ataaaacttt tggtaagtta
     1681 gttgcaatca agccaactaa actgcgaagt gaaggaactg ttgtttggga atgtcaatgt
     1741 ctgtgtggga gaaaaaagtt agtatcggca aagcgacttc taaaaggtga tgtaaaaagc
     1801 tgtggatgtt tgaaatcaaa aaagcatcac attgaaaata aaaaattcgg attacttact
     1861 gcaaagtatc ctttggacaa taactgttca tggaaaacaa aatgggtttg tcattgtgag
     1921 tgtggcagta tgtgtgagat aagttatagt aacttatttt ttggacatac tcaaagttgt
     1981 ggatgtttaa aaaggcaaga aacaaggaca tgtgttgaag ggacagtaat agagtgtcta
     2041 acgtcaaaat taccgaaaaa taatacaagt ggtgttaagg gagtttatcg ttctaaagag
     2101 aaatagattg cgtatataac tttaaaaaaa aagaggtact atttaggatc gtatgattct
     2161 attaagagtg cagctcaagc tagaaaagat gctgaaaata aattgtttca acctttgatt
     2221 gacaaatatc gaggtaatca ttaattattg tggataataa ttaacagtga tatataaaca
     2281 tctataatat attattcata ctattgatat ttagtataat cctatggaat tgaatatcat
     2341 agcaatgtat ttgaaaagca aattggaaaa caaataggtg caacaccata aatatgaaaa
     2401 aattgggaaa aagtagaatg gaaatttgct tacgtctcac gagatagctc acactattaa
     2461 tctgaagaac aatgaagtcg acttttagaa attgctagaa gaaagtaaag cgaattatca
     2521 ctagttacta tagagtaagt tcaaataaag catctccaac ttacatggtt acatgaggat
     2581 atcaaatcga aattactcaa gcttcctatg gctagagtcg cgagaagttg ttgggcagta
     2641 gacataggtt atattgttct tagctacacg atcattagaa aggaaatcct attatgtata
     2701 gttatcaaga agatgcaaaa gtccaaaaaa atcgctggtg gattttgatt gctatatcta
     2761 tgtttacctt catgtccaca ttggatggca gtattgtcaa tattgcacta cctaccatct
     2821 cagatgatat gggcatacca atgaatcaag cggaatgggt agtgtccgtt tatctgatga
     2881 ttgtctgttc ttgtttgttg ctgtttggta aaattggcga tagccgtggg aaaatcaaag
     2941 tatttcggat tggtacgctg atctttgtct tgggatcgct gttgtgtggg ttcaatcatt
     3001 cgctaggtat tttattgttc gcacgggtgg tacaagccat tggtgctagt atgactatgg
     3061 ctacaaatgc cggaatcatc acggaagtct ttcccataca tgaacgtggt cgggcattgg
     3121 gagcaatagg tgcgtttgtt tcattaggct cgattgctgg accaggtctt ggcggcttga
     3181 ttttgtccca attttcttgg gcgtacattt tttggatcaa tgtaccggtg ggaatcgtaa
     3241 cgatgttgat tgctgaaaaa ttcttaccaa aagatatcac catgagcggc catacaatcg
     3301 atggaggggg ctttgctgcc attgcggcta cgattatcag cttttttgga gcaatctttt
     3361 tggggcaaga aagtggcttt ttccatccag tcccccttgt actgttcgta atttcagtga
     3421 ttgcctttgt cagcttttta aaagttgaaa agaaagcagc ctctccattg atttctttcg
     3481 gtatttttaa gaacaagatt tttaccatga gcttgattac agccttgttg attttttctt
     3541 ccaacttctt tgtcaatgta gtgattcctt tttatctgca aaatgctcgc gggttgagtc
     3601 ctagctatgc tgggctgttg atgatggtct ttcctttgat gatggtcgtc ggggcgcctt
     3661 taagcggcta cctgaccgac cgggttggcc cggagatttt ggtattagta ggcttgggct
     3721 tattgtcggt aacccaggtc atgtatatgt ttttgtcagt ggactctcct ttgtggctat
     3781 acattgtcgc tactgccatc atgggtgccg gcaatgcctt gttccagtcc cccaataata
     3841 cgatggttat gagctcggtc actatcgaaa acttgggact ggcaggctcg atgaattcct
     3901 ttgcccgaaa tttggggatg gtcagcggga tttcgctttc tacgacggtc ttatatcaag
     3961 cgatgtctgc taagatgggc cagcaagtaa ctaactatat cgcaaatcgg ccggatgtct
     4021 ttttatatgg aatgaagatt acctttttag gcagctttgt tttgtgtttt gttgcctttt
     4081 tcctgactat ctggcgtatc aaaaaacaag gaggtattca gaaaaatgtc tgaaaaaacc
     4141 actgttgaag aatttttgga ggcctgcgta tgggtgagtg ggatttccat ggatgatgga
     4201 agatgaagtg aacttctaaa agccgaaaaa ctcaagtatt atcttcaaaa tactagtgct
     4261 caatgagatg cttgatttga tcacaagaag actgcgaaag ccgctggcaa ccgcaagcta
     4321 ggtatcccac cgatggacta cgttttagag gctactggtt atgaatacgg agccaacgct
     4381 ctcatctgca taagaataca tcacccagat tatgtaaaga ttttcgatac ttctgtcaaa
     4441 aaatcattaa gatagataaa tgatttccgt ctagtcaaat tggttcagaa tgtagacaca
     4501 cagatggtaa gaagtcactc acaattcgag aatggacttg tcctatttgc cgtatacatc
     4561 ataatcgaaa cgtcaacgat agtatcaata ttttgaccaa gggctatgaa tacaagcatt
     4621 gccttagaaa acatcaagaa ccttaggaac aacggggata gcttggttaa taagagatac
     4681 cgctgtttag gcttgcaagt atactctttt atcaagaatt ccgtggcttt agtcatgaga
     4741 ggcttaatac tatgaaaatt ttctttagca tttttgattt aggttccttt tctaattata
     4801 ataagatgac cactacagta acctactgaa atatataaac agccactgta gtaccatttt
     4861 caaagtagtc tatgagagga gaatcttcat tcttatgaac gctggaattg cggatatgaa
     4921 tttaataaaa aaaaccctta atgattttac ttccaattca atatcaaaag gtactggtat
     4981 caatttaagc acaataaaaa agctgaaaag tggtgaaaga tcagtggaaa agctgaatct
     5041 tttagatgct ataaaaataa cggaatttgc tatgaaaaat ggaaaagctg agattgaaat
     5101 atggagataa ttatcgtcta gtgaattact tgccacttag ctgaatttaa cgatccttgg
     5161 tgcttgaagg gggagattct tgggaataga gcatacttgc aagcctatgt ctttactaac
     5221 aacgatatct cttattaacc aagctatccc cgtagttcta aggcttttac ttttatctaa
     5281 gcaataatcg tattcaaagg aaatttttga cccttagaaa gtctcatttt atcagacgtg
     5341 agcttaggca ttgctgaatt ttgttaattc ggttttaggt tgtgctttga gcataatatg
     5401 aaagtattct ttatcacgat ttcattcatc taaggtaatg tgatgtgatt ctgaaattct
     5461 ttcaaaggca gtttttgcat aagctgacat tacatcatca atgacatgtt tacaatgttt
     5521 cactactaaa ataagggggt aaccaagaag aaaaactgaa tgattatttg tatctaattc
     5581 catggtatat caaccatttc ctatatagac tgattatgac atggattaaa ataaaaaggc
     5641 actatgtctt aattgtcggt tttcgagtta ccgaatggca ttcatcaccc acctatagag
     5701 gatgggcgac ttctgcctga aataagttac actacttata acttctaata aaattagatt
     5761 gagtcattat tatttaacat caacgatttt ccagtttcca tttttttgta atactaattt
     5821 gtactgaaag tagctagtgg tttttgatac attatcaagg tattttatct ttgtactcac
     5881 tagaaaattt tttccgtttt gtagataaac tggattctct aactccgaaa actttaagtc
     5941 aatattgatt ggtcgcatcg caccattttc aacataatac tccaattctt tttcagaagc
     6001 tgatggataa agtttaaaaa atgtttctaa aaaatcggtg atttcttccg ttgttttggc
     6061 atcaatttct gaatcgtttt ctacttgttt tgcttcatat tttgccttgt ctggctttcc
     6121 aatcattgtt ggcaaactag ttactaaatg attcctgttt tcatcttcaa agattgtcac
     6181 tcgatatgca gaagaaattg attgcgtctt ttttccctct gtaatatgtt gctccactaa
     6241 aaatgaaacg acaaattctt ccgagtgttt ttcaacatct agaattttta cagattgaac
     6301 ttcggaactc gtgggaatat ctgctcgaac catatcttgg attaaagcga gaccgttttc
     6361 tgtaaggtac tgttcgagat tactcattct cttttcaatt gcttctttgt tatttttcca
     6421 tgaaaaatat tctttcgcaa aatcctctgt aaaactttca acgccagaag tattcactac
     6481 cttctctttt ataaccactt tttcatgagt tgtatgttga tcaatggcgg tgaaattttt
     6541 ataaacgcca aaagcaagac tacaagacaa taaaagccaa agggcaagca ccatttttcg
     6601 atgcttaccc acggatacaa ctcgttcttt tttctttttt gggctctttt ccttttgatt
     6661 ccgttcaatt tttatcttca ttctttttcc tcttttctct tatttgtatc ggccagctcc
     6721 gataagatgc tgccgccaat agttttccgt tagattcgtc caccccactg gatctcctgc
     6781 atgaaacatg cgattctccc ctgcgtaaat gcccacatgg gtcacataag taccggcatc
     6841 ataggttgaa tgaaagaaaa ctaaatcacc tggtttggca tcttttagat caatatgagt
     6901 catggcatca tattgatcct gagccacccg aggaagtttc aggtcaactt tggcataaca
     6961 ccattgggtc aagccactac aatcaaaccc cgtttctgga cttgagccac caaacacata
     7021 ggccgttcct tcatacttaa atgcctcatc aaaaatttct tggactattt tgtcatcaaa
     7081 ctgagttgta gttaggtatt gcttcactaa taaacagtaa aacatattgc cataattata
     7141 ccgccaccca ccattttcct taatcgcgat tgggttgggg taatcgactt tttgaccacc
     7201 tgatttttcc tttgaaaaag attcagctaa agcaaaacta tgcttcttcc cgtttttccc
     7261 gacatgatct agatacccgc caccatagtt ataggcctga ataatactat cttgatcaca
     7321 ccctagttct ttggaacgct taaccaaaga cgcaaaatag agacagcctt gtttgatgga
     7381 tgcctcggta cttaatgaat taggtggtaa tccggctgac tcagaacttt gcataacgtc
     7441 ttccattgtg ccaccagatt ccacttgaat aatcgccaaa agaattggaa tatactctgt
     7501 tatatccgat tctttggcat acttttccac cattgcacgg tgtttcaata cttcttctga
     7561 gacactagtt ccgccaggag aagtgccaga actaccattt ccctcactgt cttcaccaaa
     7621 caacaaaccc acacacatca aaatacccat aaaagagatg gtcagaaaag ttaatgaaat
     7681 aacacccact ttcttcaagt tcattttttc tcacctgacc ttgattttgg acgatggttc
     7741 attctttttt gtactttttg attggtctgc tttggcagag tggacctctg tttcacttgt
     7801 tggaaagaat ttttcttact tgctgattgg cttttccctt ttgaaatgat ttcttgacta
     7861 gttagttttc gtttgatttc gggattcaga tttgctctca caatccgctt tggtgcttct
     7921 tttttcagca tcatatcttt ctgaggttga gctgctttat ctattggaat attcgccctt
     7981 ctttttccct gacgatttgt tgcttcactc agtatcttgc gtttttcgtc cattttcgca
     8041 tttcttcgtt gtcgttgttt ttctcgcaaa tccactcgat ccgctttttc ttggaacagt
     8101 ccgcgcttga attcttctgg tgcttttttc gacttttcga ttcctttatg gagattgtat
     8161 ttcaaattcg ttggcgtatt ccttacctga tcctttactt gtttagtctt atctttcacc
     8221 aaagcttggg tatccaaaac tttgccgatt tttctcccag ccaaattcat tcgggattgt
     8281 ttcttattat ttgaaactgg attctcctta gttaagtcag atagtatttc gtgatcgttc
     8341 ggtaatcgtg aattctttcg tagagaagaa ccggatggct taaatttgga ttttgattgt
     8401 tcctttgcca acttatttcc gactgctgca ccaccaccaa aagccaaagc tcgtcccaca
     8461 tttcgattga gttgccgcaa ttttcgattc attaacattt gcggttttcg catgacgcgt
     8521 cttcccattg actggctatc attactctgg aggttgaaca tactcataat gtcgcccaat
     8581 ttgaaataga taccggcaaa ggtcacaatt tgcagaaaag caaccatgaa aaatggatag
     8641 gttgccgaaa tattgaaaaa catcgtggag atgctaaatg cagtcgtaat caccaaagta
     8701 acacccgctc gcatcataat cgtattgaat aaacgaataa tcgccttctt tcctaaactt
     8761 tcataagtcg gcaacatgga caataagaaa ctaattggca gaaacatcgc aaagataatg
     8821 aagagaattt gcgagaacaa cataatccct gttagaagaa aaacaaagaa ggaaatgcca
     8881 atattgaaca ataccaaaaa gaccacggtc cctaaacggt tcatggtctt ggtaatactg
     8941 agattcgcat tgtcattgtc ttcaatctct gccttgaccg cttcttcccg atctttgcct
     9001 ttgttttcat ctggactcac agatagaatc ttgttgaccc ggtcttcacc aatttcttct
     9061 atgttggaat catcaaattg cagtagtaac caaggttgtt ggacttgaat cgaaaacaaa
     9121 ctatcccgaa tcaaatccac actatctttt ccttggcttt ctgaattggg tacaacgatc
     9181 tcggttccaa gggtaagggc cgcttcacta acatctgagc taaagtcatt gatttttgtg
     9241 atataggtag gcgcataagc aatgaacgaa cccgatagta gaaaaatcat cacaaaatta
     9301 atcacggctc gaactgcttt cgtggtttct cgttttaata atccggtata cgccacatac
     9361 acccccagag ctaaaatcat caataataga aacccaaaat aaaatccgga ggtctggagt
     9421 ccattttcag taataccagc caatgtttgg atattctttc caatactttc agccgtatcc
     9481 gaaatgaaat ctaatttgta agcctcttga accacataac ccgtggcgtt cgacaaatac
     9541 aaactcaccg tccaaatgaa attagtaatg gcataaagac catacatcac gcttttccct
     9601 aagccatcac cccaattcca aggcaaccaa tcccaagagc tatccacaaa gaaatctagt
     9661 tgatagttgt tcagtgaata tttggaatat agatttcctg cttcaactgt atcatcaact
     9721 agccctgtcg cctcggctac cgtaccaact aaactcaata gaactatcag agtgagtatg
     9781 ctaataaaaa cgattcccac aatccgaaaa agtttctttt tcatcccctc actccattct
     9841 ctcttgaaca ggtggacggg tatcaaaggc atggaagagt tcttcaaaga ctggatgtac
     9901 ctgaaccacg cctacccgac catacaaatc ctgcatcaag cattggccat tttccaaatc
     9961 tcgtaggcgt ttttgattgc tttcatcttc tcgatccaag ccaaagaatt ccaaggtttt
    10021 cttgatttca tgaatatcgg tactgcgaaa cgcaaacttc aacccgatat tattcttcaa
    10081 gcgttcatcc agtaaatcat cactattttg cgtgacaaag taaaccccgg cattcatagc
    10141 ccgcccagca cgaacgagct tattcgataa tgttttccct tgcgctactt gaagaaaact
    10201 ccacgcctca tctaagtcta caattttgaa gatttctcga ttactatgga taaagtccaa
    10261 ggcaaaagtg gaaatgacga ttagcatgga aacactaagc aattccatgg tggtatattc
    10321 ttcaaaagtg gtttctgcat ctggtaaaac caaatccgct acttgaatga tatttaactg
    10381 cctatctaaa ctaatcgact gggtaatcga accatctgag aaaagcaaat gggcaaaatc
    10441 ataatcggta aaggactcga tgtgatccgc gatattttcc gcaactggtg agccttcttt
    10501 tcgaagctca ttaattactt gtaataatcc ccgttgattg ctttgggtaa ccgtccgaat
    10561 ggccttacgc aacactggaa acttctcccc atctcgacta gaaattcctg ttaaaaaggt
    10621 cagaatatca attgctagac tttcagaatc cttctttttc tccataatca cataaggatc
    10681 aagtaatcct tgattttttg gcttactagt taagttgaga atattgattt catccgcaat
    10741 ttctggcaag gtttctttcc agccaccccg ttcccctttt ggatcgacaa tcacagcttg
    10801 tccaccaaaa aggacggcgt aataaactaa gagattgtta ctaaaggact ttccgccacc
    10861 gagtgaaccg aggaatgccg ctgctagcgc attggtaact gaatccttga ccccttgtga
    10921 ggccaatgcc ggtttcaggt aaacattgcg ccccgtatct acgttataac caaagtaaat
    10981 gccttccaac tcccctaaca tctgagtggc gccaaaacca agtccggcga gaaaatcaga
    11041 cgtaacatac tggatataat cattcatgta tcgcttacta gaaggtaaga attcttcatg
    11101 tagccccatc atatcgccaa aaggccggac tagttttaca ttggtatcat cataaaaatc
    11161 tagcacttca tcacaccggc gctttagttc atctactgat tcggcactta ctcgaaccac
    11221 atagctcagt ttgtacatgg attctttgga ctgatccaag gtagtttcca attcatctac
    11281 ggaatccaat gcatctaaga cattggaatt tgtttcattg tcagattgat aggcatggtt
    11341 gtccaagtct tttaattcct ttttcttatt gcgaacggta gcaagtgcct ttttgttcgt
    11401 cacaatctcc acattcatac tagtgtcaat tgggaatgta aattgttgtt gctggtaata
    11461 gaaaagttct gaggagggaa actccatttc ccccacaatt gtattaattg ttaagtacgc
    11521 tacatatgat tcatgattct catgttccat gcataagtac cggggctttt cttcaatcaa
    11581 acaacgactg ggacgtaata agtcgtaacg tttcaccaaa gtttccgact tcagcttttt
    11641 ctttggtagc tgaaattcat attcttcaaa tggtgtaccc ttttcgccat agatatgttc
    11701 aatcaaatac atcagatcac tcggtgtgac tcgtctgact ttaaatcttc gagccaattt
    11761 actttccatt agtttttcaa gcttggtata tcggcgaatt tcttcattgg ataaggaaac
    11821 aaaatcgccc atcaaatgat gattcactcc atagacaaat tcttgaaagc cggagaagaa
    11881 agatttcttg agattcttta gattgacctc ttcatctgtc gcaatcagtt tgaagccaat
    11941 aaaaaagcga taatcaatct gactatcgcc aatcatggat actaatgcct ctgtctgtaa
    12001 atcaattcgt tgtttcgcta cttctgttaa tcgcccggta atttcctttt ttgatcgttc
    12061 ttgcgtggca cgaatactac tttctgtggc aatttgtaag gcatgaatct tgccttcacg
    12121 attttgcgca atcaattgac gaaagttatc gtggacttga tatttctgtt caggtgacaa
    12181 aaaggagtaa ttgtaaggta ttaactcata gtaggcaaaa cattctccct cttgattaaa
    12241 gacaagatta ttttcaatat acttaatcgg gtacatagtt cacactcctt accagagtaa
    12301 tgggttcgtc ccatcgtttc ttctgttctt tgactttctt tcctgcgaaa gtgaccttaa
    12361 atcgtaagtg atagctaata aaagatcgta aaaatcccaa tggtttctta ccgtcaaacg
    12421 ttttttgcga catgaaccaa gtaactgcca ccggtatacc taagtatttt aaaaaggccc
    12481 cctctatcat agatagtgga ggcacatggg caaatacgat gacaaacagc aaggataaaa
    12541 caaaccaagc catctggtta aaagtcacag gaaaaggcag ttgaaagtca ttaattgcat
    12601 aaatgacctt ttcaactgcc caaatactgg tgtagctttt tattttcttc atcaaatcat
    12661 tcctttaaaa agaaggatag tttagaaatc aactaccctt caataatttt gttaatatgg
    12721 atactcaaaa acacctcgac tcgttaccaa ataattgcct ccaatctcca agtctctacc
    12781 acacgcatca aaatcaatat acgtcttcaa gtgttctggt acttctccaa aatcaccagc
    12841 ttctacaatc aagaaatatg ctacatcact catatcatcg caatctggat aatagataat
    12901 atcatccaca tgctctacca tctcctcaaa agaattgaag aaagcatgtt gaatctcaga
    12961 agcaacttca ccaactggcg ttccttcaag ttcctccgct aagccacaca accgattaat
    13021 ttcctcaatt ggcgtatact catcaatctc aaagggaagt tcataatcat gaattgcata
    13081 ttcctcgtat tcgtcattta acccaattcg ttctttcact tcatcaaaat cgacaggtgg
    13141 ggtaaaccac gcccccacca attcaccttc gttatatttc ccgaggttcg caatataaac
    13201 acgcatctgt tccatcgtgc ttccccctta cgcaccgaca attcggttaa acaaactgag
    13261 taagacatcc ttcaccccag aagcattaaa gaccaatccg accgcaatca aagcaatcac
    13321 caaaaagcca atcagtttgg aaaactcccg cttaaatcct aaaaacaaac caatgaccac
    13381 aattgccagt aagactaagg attg
//
LOCUS       DARBET010000069        13399 bp    DNA     linear   BCT 07-NOV-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4027
            SAMEA114569841-rid20112183.denovo.071, whole genome shotgun
            sequence.
ACCESSION   DARBET010000069 DARBET010000000
VERSION     DARBET010000069.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA114569841
            Sequence Read Archive: ERR12203666
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 13399)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13399)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001972618.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001972618.1
            Genome Coverage       :: 132x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/07/2023 13:18:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,041
            CDSs (total)                      :: 2,975
            Genes (coding)                    :: 2,908
            CDSs (with protein)               :: 2,908
            Genes (RNA)                       :: 66
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 67
            CDSs (without protein)            :: 67
            Pseudo Genes (ambiguous residues) :: 0 of 67
            Pseudo Genes (frameshifted)       :: 26 of 67
            Pseudo Genes (incomplete)         :: 28 of 67
            Pseudo Genes (internal stop)      :: 20 of 67
            Pseudo Genes (multiple problems)  :: 6 of 67
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13399
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA114569841-rid20112183.denovo.071"
                     /isolate="VRE4027"
                     /db_xref="taxon:1352"
                     /country="Denmark"
     gene            complement(15..707)
                     /locus_tag="SCJ86_002211"
     CDS             complement(15..707)
                     /locus_tag="SCJ86_002211"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005874227.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase"
                     /protein_id="HEG1169735.1"
                     /translation="MQIELQHTHDKETFEKNYKVYFELARDSAMKYGIPLTLDTPYNQ
                     PGIKSHLWVTQNIWGDHTDPYGYLSEMGVSKEKLAYDLAHGFTDDNPTTSEDKPVIDP
                     TRAGAANSTLTDGTNYAHIDQFGEIENANLHVAGWHIANYKYEYIFIMDYNTGKELAR
                     VRADGIYRPDVNQAYNTLGNVGYHVSFNMRDFPNKKVYVMMRATNDPEGNTKGGAQDF
                     HDKRWYLNIPQR"
     gene            complement(1144..1713)
                     /locus_tag="SCJ86_002212"
     CDS             complement(1144..1713)
                     /locus_tag="SCJ86_002212"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294835.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system Phd/YefM family
                     antitoxin"
                     /protein_id="HEG1169736.1"
                     /translation="MELKKLEVPTSSITEVKRSPMDVFAQARDAGTGVYIFNREKVAG
                     VMLTQEQYETLLQELDVLRQTVKVERKEPKEINEEPINKTASVVSNAEGMEELAQTLQ
                     HSLITGVTITAKNLDERMVALGFITKKTGFGGVVDMLSELMETGKINYQLRRKPQNRT
                     IIAEIIGEQNSQSQLMDKIIIKKIFLHEL"
     gene            complement(1787..2110)
                     /locus_tag="SCJ86_002213"
     CDS             complement(1787..2110)
                     /locus_tag="SCJ86_002213"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002356126.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEG1169737.1"
                     /translation="MLLLKTLQERGYLDVHPPEVKIVFFFRYEHQEARRLAGVLNKTL
                     RHRKRISIHVCTRNKPPRYADLVIIDHFFPLDHNEDQKTYLYHPSFGIERLLTDINYW
                     LGKNR"
     gene            complement(2264..3076)
                     /locus_tag="SCJ86_002214"
     CDS             complement(2264..3076)
                     /locus_tag="SCJ86_002214"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008274430.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEG1169738.1"
                     /translation="MRWQKLLSKNAQNLFSLINLMKDLIWRKADLAEELKIDVKTVDR
                     HLKRLAESDCPIQLILHKKTVQLVYDQGYSEPYLLFVFLIQSPPFCLLKNLLLNEPYE
                     QKYDRSTQERLRKWFGEYHLSFSYKKAQIFGSERKVRYLLFCFLKDFPHFFADDGEQS
                     FSEMFIQLLKQRKDPAEIEILGDSRTLFFELFPELLFREEQILPIKEKNYLAILRLSC
                     NQITEKRWKRIEETQDYRKLTIVTEKLDSLFWLNSEFESESKSKLTKVLYQE"
     gene            3430..4278
                     /locus_tag="SCJ86_002215"
     CDS             3430..4278
                     /locus_tag="SCJ86_002215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008274428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MoxR family ATPase"
                     /protein_id="HEG1169739.1"
                     /translation="MIVALLAGGHVLFEDIPGVGKTLLVKALAKAVQGTFSRVQCTPD
                     LLPSDILGFSVYNSQSKEFEFRPGPIFTTILLADEINRTTPRTQSALLEAMAENHATI
                     DNNTYPLDNHFFVLATQNPIEYEGTYPLPEAQLDRFLFRLQIGYPSFDDELLLLLDKF
                     EKVLDNLNEVLNSYEIEEIKTNVANVFVSPEVASYALQLVSATRSHAAIQLGISPRGS
                     LSFIQAAKAYALIHGRNYVTPKDLQDLVPYVFSHRLIFYDRSLKEDEKKQIIHTLVAQ
                     IPIPVR"
     gene            4423..5364
                     /locus_tag="SCJ86_002216"
     CDS             4423..5364
                     /locus_tag="SCJ86_002216"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304699.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF58 domain-containing protein"
                     /protein_id="HEG1169740.1"
                     /translation="MELDFIKQLSKETFGYLYIAATRLSQKKYAGYFFHSKKENFFFT
                     SPSFTNRLPSNLCRKKQEILILTNKPQEVQLLWQPKERGCHETLTVIFTAYDSLGLFR
                     KSAKREISFPVTVLPAFCKIQAEKLQRVLEKKQQLNAYQRGLDFREHRAYRPGDSFNR
                     IDWKLSAHTQEWLYREYDYQEEECSPLFCFWGLPSPKFEPLLDLYFSLWHLRKEKQPE
                     LLILGNRAFHGKDPGHTYFASLEAGDEKAFFAHLKKYAKKQIVLFVPENSPEVSEAIE
                     TLSKDRTVYLVYFVEKQLMVQEKDKICSLPGGEWIDD"
     gene            5354..7525
                     /locus_tag="SCJ86_002217"
                     /pseudo
     CDS             5354..7525
                     /locus_tag="SCJ86_002217"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343611.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transglutaminase domain-containing protein"
     gene            complement(7637..9808)
                     /gene="copB"
                     /locus_tag="SCJ86_002218"
     CDS             complement(7637..9808)
                     /gene="copB"
                     /locus_tag="SCJ86_002218"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376511.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="copper/silver-translocating P-type ATPase CopB"
                     /protein_id="HEG1169741.1"
                     /translation="MNGNDMEKKHEHKKEVVNKESTESHEKQMEMKHDHSQMDHSMHM
                     GHNHGDIANSKQMDHSMHMDHEHGGMDHSMHMGNFKQKFWLSLILAIPIILFSPMMGM
                     EFPFQVTFPGSDWLVLILATILFIYGGQPFLSGAKMELKQKSPAMMTLIAMGITVAYI
                     YSVYSFIANLLNPHTHVMDFFWELATLIVIMLLGHWIEMNAVSNASNALQKLAELLPE
                     SVKRLTKDGKEETVSLKEVNEGDRLIVRSGDKMPTDGVILKGETIVDESAVTGESRGI
                     KKQANDKVIGGSINGDGTIEIEVTGTGENGYLAKVMEMVRKAQGEKSKLESLSDKVAK
                     WLFYVALIVGILAFIAWLFLTDLPNALERMVTVFIIACPHALGLAIPLVVARSTSIAA
                     KNGLLLKNRNALEQANDLDVIMLDKTGTLTEGKFTVTGVEVLDDAFNKNEILQYLGAL
                     EANANHPLAVGIMNYLKEHEIKPYQAENLKNLSGVGLEATVKKQQVKIVNEKEVERLG
                     LNVEQALLKPYQEQGNTISFLILENHLAAIVALGDVVKTEAKEFIRTLKERKITPVML
                     TGDNKNAAQAVADYLGIEEYYGGLLPDDKEAVVQKYLDQGKKVIMVGDGINDAPSLAR
                     ASIGMAIGAGTDIAIDSADVVLTNSDPKDILHFLDLAKQTRKKMIQNLWWGAGYNILA
                     IPLAAGILAPIGIILSPAVGAVLMSLSTVVVALNALTLKIR"
     gene            complement(9828..12014)
                     /locus_tag="SCJ86_002219"
     CDS             complement(9828..12014)
                     /locus_tag="SCJ86_002219"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010737925.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="copper-translocating P-type ATPase"
                     /protein_id="HEG1169742.1"
                     /translation="MATAETKMDTFVITGMTCANCSARIEKELNGQPGVVNATVNLAT
                     EKASVKYEGTTTEKLIQSVENIGYGAILYDEAHKQKIAEEKQAYLKKMLFDLILSTVL
                     TLPLMLSMIAMMLGSHAAIVHFFHFPIVQLVLSAPVQFYVGARFYKGAYHAIKTKAPN
                     MDVLVAIGTSAAFALSIYNGFFRGHPQDLYFESSSMIITLILLGKYLEHTAKTKTGNA
                     IKQLMSLQTKTAQVIRNGKEETLAIEEVVVGDQLVIRPGEQIPADGRIISGSSAIDES
                     MLTGENLPVEKNPDDTLFGGTINTNGLLHMEVTQVGKQTVLAQIIQMVEDAQGSKAPI
                     QKIADRISGIFVPIVLVIAFITLIATGLITGDWQLALIHSVSVLVIACPCALGLATPT
                     AIMVGTGVGARNGILIKGGEALEAAAHLDSIVLDKTGTITEGKPKVTDLVGSKEVLSI
                     FYTLEQASEHPLGKAIVEYGKLQEAATYDMIDFTAHPGAGISGTINGVRYFAGTRKRL
                     IELNLSFDEYQEHALELEQQGKTVMFLADEKQVIGLIAVADQIKLEVKQAIKQLQNKG
                     LDVFMLTGDNKLAAETIGKQVGIDPKHIFAEVLPEDKAAYVEKLQKDGKKVGMAGDGI
                     NDAPALALADVGMAMGSGTDIAMETADVTLMNSSLASIAQTIELSRLTLRKIKQNLFW
                     AFVYNTIGIPFAALGFLNPIIAGGAMAFSSVSVLLNSLCLNRHMKK"
     gene            complement(12014..12223)
                     /gene="copZ"
                     /locus_tag="SCJ86_002220"
     CDS             complement(12014..12223)
                     /gene="copZ"
                     /locus_tag="SCJ86_002220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376514.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="copper chaperone CopZ"
                     /protein_id="HEG1169743.1"
                     /translation="MKQQFSIEGMSCNHCAARVEETVSALDGVQKVKVNLKKANGTVK
                     FDETKVQSEKICQAINGLGYKAEVI"
     gene            complement(12236..12676)
                     /locus_tag="SCJ86_002221"
     CDS             complement(12236..12676)
                     /locus_tag="SCJ86_002221"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333694.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CopY/TcrY family copper transport repressor"
                     /protein_id="HEG1169744.1"
                     /translation="MEKVKTPVKISDSEWEIMRVIWTLGQTTAQEITQILADSMDWKP
                     ATIKTLLGRLVKKEVIWTEQEGKKFIYYPAVSEMETVRSATENLFSHICAKRIGETIA
                     DLVEEATLTQEDINEILKQLEQKQPVDTIECNCIPGQCECKQHQ"
     gene            complement(12750..13289)
                     /locus_tag="SCJ86_002222"
     CDS             complement(12750..13289)
                     /locus_tag="SCJ86_002222"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853035.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HEG1169745.1"
                     /translation="MKIIVIGQSGSGKSTLARKIKEITDFPLLPLDLLWHTTDYSIQA
                     KKWFLQEQQLFMVSNPSWIVEGNYTSTLEERIKEADKIIWLKVPRYLAIYRVVWRSLK
                     RKINKKSRPDMPESFSEKLNREYLEFLSFIWHFEENNEPKIQQLIQVANARNKLIILK
                     TRREKQEFLTKLKIESKNN"
BASE COUNT         3765 a         2807 c         2381 g         4446 t
ORIGIN      
        1 gaggggggga ttttttatcg ttgtggaata tttaaatacc aacgcttgtc atgaaaatct
       61 tgtgctccac ccttagtatt tccttctgga tcgtttgtcg cacgcatcat gacgtatact
      121 ttcttattag ggaaatcacg catattgaaa gatacatgat agccaacatt tcctaaagta
      181 ttataagctt gatttacgtc tggtctataa atcccatcag ctcttactct agctagttct
      241 tttccagtat tgtagtccat aatgaaaata tactcgtatt tataattagc aatgtgccat
      301 ccagctacat gcaagtttgc gttttcgatt tctccgaact gatcaatgtg ggcgtaattt
      361 gttccatctg tcagcgtaga atttgcagca cctgctcgag ttggatcaat tactggttta
      421 tcctccgaag tagttggatt atcatccgta aaaccatgag ccaaatcata ggctagtttt
      481 tctttactta cacccatttc agaaagataa ccgtaaggat ctgtatgatc gccccaaata
      541 ttttgtgtta cccataaatg cgatttgatt cctggttggt tataaggagt gtctaatgtt
      601 aatggaatac catatttcat tgctgaatct cttgccaatt caaagtatac tttatagttc
      661 ttttcaaaag tttctttatc atgtgtatgt tgtaactcga tttgcacagg acttttggct
      721 cacgaggttg tcctactcta catcttgctg atagatatgc tgaaaggaaa acaccaacat
      781 tatgttttgc tttttcagct agaacgagac aagcaggaat acctttagaa gatatcaaag
      841 gttttttagg tcataaagat gtttccacta cacaagtcta tgcccatatt tctcccgaag
      901 tgaaaaaaag atcaatgaat caactagaaa actatataga agagcaaata aaaaagcact
      961 caaattgagt gcttttttaa gctcactatc actaaaacta tcactaattt tatttataag
     1021 cgaacatgtt tctatcactg attttttaaa tctagcgaaa aacccttatc ttaaaggcgt
     1081 tcgttcaatt ctttggcaag gtcttcaaag cctggcttgc ctagaagtgc gaacatggtt
     1141 ttcttatagc tcatgtagaa aaattttctt gatgatgatt ttatccatca attgagattg
     1201 actgttttgc tctcctatga tttcagctat gattgtccga ttttgcggtt ttcttcttaa
     1261 ctggtagttg attttcccag tttccatcaa ctcgctaagc atatccacga ctccgccaaa
     1321 tccggtcttt ttcgtaataa aacctagtgc taccattcgt tcatctagat tttttgctgt
     1381 aatcgtcaca ccagtaatga gcgagtgctg taatgtttgg gccagctctt ccatcccttc
     1441 agcattggat acgacggaag ctgttttgtt aataggttct tcgtttattt cttttggttc
     1501 tttcctctcc actttcactg tttgacgaag gacatctagt tcttgcaaga gcgtttcgta
     1561 ttgttcttgg gtcagcatca cgccagctac tttttcacga ttaaatatat atacgccagt
     1621 tcccgcgtct cgcgcttgag caaatacatc catcggcgat cgtttcactt ctgtgatcga
     1681 agatgtggga acttctaatt tctttaattc caatggattt cctcctttac taatcgcttt
     1741 ttattatagc acagactgac ttgttttgat cttttttcca aaggttttat cggtttttgc
     1801 ctaaccagta attgatatcg gtaagaagtc tttcgatacc aaatgaagga tggtacagat
     1861 acgttttttg atcttcgtta tggtctagtg gaaaaaaatg atcgatgatc actagatctg
     1921 cataccgagg cggcttattt cgagtacata catgtataga gatccgtttt cgatgtctta
     1981 gcgttttgtt cagtacccca gcaagtcttc ttgcttcttg gtgttcatag cgaaaaaaaa
     2041 agacgatttt cacttcagga gggtggacat ctaaataccc tcgttcctgg agtgttttca
     2101 gtaaaagcat cccccactct actgcggggt gtgcaagtgc ttgttggatc gctagccgaa
     2161 attgatatag tttatctttg gaattgggaa atttttctaa tgtttcctga taataacgaa
     2221 cttcttctat cagccggtca tggataccaa ttacgtaacg aagtcattct tggtaaagta
     2281 cttttgtcag ctttgatttg ctttcgctct caaattcgct attcagccaa aacagcgaat
     2341 caagcttttc agtcacaatc gtcaatttcc gatagtcttg tgtctcttct atccgtttcc
     2401 accgcttttc agtaatctgg ttacagctca agcgcaaaat tgctagataa tttttttctt
     2461 ttattggcaa aatctgctcc tctctaaata gcaattcagg aaacaattca aagaatagtg
     2521 ttcgactgtc tcctaaaatc tcgatttctg ccggatcctt tctttgcttc aataattgga
     2581 tgaacatctc agaaaaggac tgctctccat catctgcaaa aaaatgcgga aaatctttca
     2641 aaaagcaaaa cagcagatat cttacttttc tttctgaacc aaagatctgt gcttttttat
     2701 aagaaaaaga aaggtggtac tcgccaaacc actttctcaa tcgctcttgc gtagaacgat
     2761 cgtacttctg ctcgtaaggt tcattcaata gcagattttt cagtaaacaa aacgggggtg
     2821 attggatcag aaaaacaaac aagagataag gttcgctata tccttgatca taaactaact
     2881 gaacggtttt cttatgaaga atcagctgaa tgggacagtc gctttccgcc aaacgtttca
     2941 gatgccgatc cactgtttta acatcgattt tcagttcttc agctaaatca gcttttcgcc
     3001 agatcaaatc tttcattaga ttgattagtg aaaataaatt ctgtgcattt tttgataata
     3061 gtttttgcca tctcatcgat catttctcac ctgatttcta aacatattgt ttgatcaaaa
     3121 tgggttcact cctaaaaaga taaaaaactc cctttctatt tatttaaata cgcaaatctt
     3181 cctcattatt ttttatttta ctcaatttaa tcgtattctc atttcaaaac gaatgtactt
     3241 taaaaaaaca taataagatc actttgcttg tatgatgtat tcccttccat tacaatgaaa
     3301 taaatagcga ttttatgaga aggtgatgtg aaaatggaag aaaaaataga agaatccaaa
     3361 caaaaaatcg atcagttgat ataagaatta gaaaaagcgg tcatcagcaa acgttcaact
     3421 atccaattga tgatcgtggc cctattagca ggtggtcatg tcttattcga agatattccc
     3481 ggtgtaggta aaacgttact ggtcaaagca ctagcaaaag ctgtacaggg aaccttcagc
     3541 agagtccaat gtactcctga tctgctgcct agtgatattc ttggtttttc cgtatacaat
     3601 tcacaatcaa aagagtttga atttcgtccg ggaccgatct ttaccacgat cttgttagca
     3661 gatgagatca atcgtacgac tcctcgtact cagtcagcac ttttagaagc aatggccgag
     3721 aatcatgcaa cgatcgacaa caatacatac cctctagaca atcatttttt tgtcttagct
     3781 acgcaaaatc caatcgagta cgaaggaaca tatcctttgc cggaagcaca gctcgatcgt
     3841 tttttattcc gactacaaat cggttatcct tcttttgatg acgaactttt gctacttttg
     3901 gataaattcg aaaaagtgtt ggataactta aatgaagtat tgaacagtta tgaaatagaa
     3961 gaaataaaaa caaatgtggc gaatgtcttt gtcagtcccg aagtggcatc ttatgccttg
     4021 cagttagtgt cagcaacaag aagtcatgct gccatccagc ttggcatcag tccacgagga
     4081 agcctttcct ttattcaggc agcaaaagct tatgctttaa tccatgggag aaattacgtc
     4141 acgcccaaag atttgcagga tttagttcct tacgtgtttt cccatcgcct gattttttac
     4201 gatcgttcat tgaaggaaga cgaaaagaaa caaatcattc atactcttgt tgcacaaatt
     4261 cctattcctg tgaggtagat tgaggtagac aatggaaaaa atcaaagatt atttcattaa
     4321 ttttgtgtta tttctggtat atttcatcat tgtgcttttt gctcttgtat tcaacaattc
     4381 tttaggctgg ttcttgttgg ccttttttac tctttatgta tcatggagct tgatttcatt
     4441 aagcagcttt ctaaagaaac tttcggttac ttgtacattg cagccactcg tttatcgcaa
     4501 aaaaagtatg caggttattt tttccatagc aaaaaagaga actttttctt tacctctccc
     4561 tcgtttacaa atcggcttcc cagcaatctt tgcagaaaaa aacaagagat tttgattctt
     4621 acaaataaac cgcaagaagt ccaattgctt tggcagccaa aagaaagagg atgccatgaa
     4681 acattaactg tcatttttac agcctatgat agtctaggac ttttcagaaa aagtgcaaaa
     4741 agagagatct cttttcctgt gactgttctg cctgcttttt gtaagattca agcagaaaaa
     4801 ttacagagag ttctggaaaa aaaacagcag ctgaacgcgt accaaagagg tcttgatttt
     4861 cgggaacatc gcgcttatcg tccaggtgat tctttcaacc gaatcgattg gaaattgtct
     4921 gctcatactc aagagtggtt ataccgtgaa tacgattatc aagaagaaga atgttctcct
     4981 cttttttgct tttggggttt gcccagtcca aaatttgaac ctctgctaga cttgtatttt
     5041 tctttatggc atttgagaaa agaaaagcag ccggaacttc tgatattagg caaccgcgcc
     5101 tttcatggca aagaccccgg tcatacctat tttgcttctt tggaagctgg cgatgagaaa
     5161 gcttttttcg cacacttgaa aaaatatgca aaaaaacaaa tcgtcctctt tgttcctgag
     5221 aattctcctg aagtctcaga agctattgaa acattgtcaa aagatcgaac ggtttatctt
     5281 gtttatttcg tagaaaaaca actcatggtc caagaaaaag acaagatatg ttcattgcca
     5341 ggaggtgaat ggattgacga ttaaacggga aaaatggctg cgtggcgttt actcctttgt
     5401 gatactaagt gtaggtgctc cgatatttgt agtagctaat caattgcaaa acagctacct
     5461 tcttatccta actattggtg tcctatgcct tattgtctgc tgtattccta aaaaatgggt
     5521 acgtttgctg ctttctttgc cagtaatgat aggttgtttg tttttatatt ttccttcgaa
     5581 gacattttcc atattatgga tcttacagtt tttttcgtca gtcacagagg aattcttcca
     5641 tctatcccaa ggtaaacttt attatttgcc agaaaagaca gctttttttc tcataatgtt
     5701 gctgatctat ttatttatta cattgaccgt ggattacgac tattggtacg caccctttct
     5761 ttctttgatg gcttacttca tgatgcttac cgtttttcgg aaaaaagatt tactgtacga
     5821 aatgattgcg gtagtaactg ttttgttcgt gtatctggca gcaaggcaat ttttctccat
     5881 ccggttgttg tcgggaaaca ctcgattcca gtcattgact acagttctgc ttatttgttt
     5941 cttaactttt agcgctatct tgcctttata tcttccgaaa gtacaccaat ctctcgagga
     6001 aacgagtgaa ccgatccgag aatatcttaa tgatgaaggg ctttatgaca cactccatga
     6061 gtatcaatta accggaagtg caagaacagg cttcagtgag aatgatgaac agcttggtgg
     6121 acctttatat gataatggcg aagttgtttt tacagcgaac caaaaaggaa atggctattg
     6181 gaagatcgaa aataaaaatc attacaccgg ttctggatgg atagaagatc aggacgcacc
     6241 catccaacca atccaagaat tccctgttga catcacgagc taccaaaccg ttatagaaga
     6301 caaagaaaac attacattga ccctaaaaaa acaagttttc ttaccttatc cttatggaaa
     6361 tactaccttg actgattttt cattagctag tggacaaacg ctttactatt tgcctgaaag
     6421 ccagcgattc attcctgtcc cgaatgaatc gacacgttct gcaactttac aaacagaaag
     6481 gttagttttt acgagagaag aattaaaaag cagtcagtta aaagctgatg atttagaaaa
     6541 tcagcagaca aatcttcaat tgccaaaaag tttgcctaaa cgtattgagg acttggcggt
     6601 cgaattgacc aaaaatgaag caactttaat tcgtaaagtc gaagctgtct aacagtattt
     6661 aaaaacaagc ggaaatttcc gctattccaa aacggatacc gggcatactc caaaagaaca
     6721 cgattatgtg gattatttcc tctttgattc aaaagtcggc tattgtgaca acttttcaac
     6781 ttctatggtc gtccttcttc gaacacttgg tatcccagct cgctggacaa aaggttttac
     6841 aaatgggctc agaacaggca cagataatca agggaatgag gaatacagca ttctcaatag
     6901 tcatgctcat tcttggccag aggtatactt tcctggattt ggctggcttc catttgaacc
     6961 aaccccttca tttgccaatc caatgggttc acaaccagtt gctgaaacaa ccgaaaccgt
     7021 ccagcctgct atcgaacaac caaatgaaag cgaaaatatg gcagaaacta tttccagtga
     7081 gagatctgag gtcattccat cagaagaaac aacactcaca ccagcttata aaatcgtctt
     7141 attcagcctt ggagtgtgtt tatccgtctt gttgttcttt ctattgttat tcagaaaatc
     7201 tctgcgctgg acaagttatc ttatctatct tcattttttt gcttctacta atacgattgc
     7261 atatcaaaaa cttcttaacc ctttggaaac attggtaccg cgcaaacccg cagaatcttt
     7321 gcaggaatat agcctgcgaa tagaacaaga attcaagcta gatctgcgtt tcattcgatt
     7381 aactactcaa tatgaagcag aaatatatgg acgacaagca gatccggtaa aattcgataa
     7441 aaaaacctat ttagaaataa cgcatataat aagaaaaatg gtatacaaaa aaattctgac
     7501 aaaagggagc cgcttcaaaa aataagataa aatttctgga gaatctactt ctggaatgcc
     7561 gagaaccagc tgattgagta tcgattactc gaaaaaaagg ttgtgacacg ttttttgtca
     7621 caaccttttt tcattattat ctgattttca atgtcaaagc atttagagct acaactaccg
     7681 tacttaacga catcaagaca gcaccaactg ctggacttaa tatgatacca attggcgcaa
     7741 gaattccggc tgctagcgga attgcaagaa tattgtaacc tgccccccac cagaggttct
     7801 ggatcatttt ctttcgcgtt tgcttggcta gatcaagaaa atgcaaaata tcttttggat
     7861 cgctgtttgt caatacgaca tccgcagaat cgattgcgat atctgtccct gcaccgattg
     7921 ccatcccgat tgaagcacgg gctaaacttg gcgcatcatt gatcccatca cccaccatga
     7981 ttactttttt cccttggtca aggtatttct ggacaacagc ttctttatca tctggtaata
     8041 aaccgccata atattcttcg atccctaaat aatctgctac tgcttgggca gcgtttttat
     8101 tgtcaccagt aagcatgaca ggagtgattt ttctttcttt caatgttcgg ataaattctt
     8161 ttgcttctgt ttttactacg tcacctaaag caacgattgc agctagatga ttttctaaaa
     8221 tcaggaagct gatggtattt ccttgttctt ggtaaggttt caataatgct tgttctacgt
     8281 ttaatccgag acgctcaact tctttttcat taactatttt cacttgttgt tttttgactg
     8341 ttgcctctag cccgactcct gaaagatttt ttagattttc agcttgataa ggcttgattt
     8401 catgttcttt caaataattc ataatgccaa cagccaatgg atgattggca ttcgcttcca
     8461 aggctcctag atattgaagg atttcatttt tattgaacgc atcatcaagt acttcgacac
     8521 ccgttacagt aaattttcct tctgttagtg ttcctgtttt gtccagcatg atcacatcta
     8581 gatcattggc ttgctccagt gcattgcggt ttttcaaaag taatccgttt ttagctgcta
     8641 ttgatgtgga acgagcaaca actaatggga tagctaaacc tagagcatga ggacaggcga
     8701 tgataaagac tgttaccata cgttctaatg cattaggaag atctgtcaag aacaaccagg
     8761 cgatgaatgc caggataccc acaattaatg cgacataaaa cagccatttt gccactttgt
     8821 cagataatga ctctagcttg gatttttctc cttgtgcttt tcgcaccatt tccataacct
     8881 tcgctagata accattttca ccagttccag ttacttcgat ttcgatcgtt ccatccccat
     8941 tgatagaacc gccaatcact ttgtcattgg cttgtttttt gattcctctt gattcgccag
     9001 tcacagctga ttcgtcaaca attgtttcac ctttcaagat tacaccatct gttggcattt
     9061 tatcaccaga ccggacgatc aaacgatcac cttcgtttac ttctttcaat gaaactgtct
     9121 cttcttttcc atctttcgtc aagcgtttca ctgattctgg cagtaattct gctagttttt
     9181 gtaaagcatt gctggcattc gacacagcat tcatttcaat ccagtgacct agtagcataa
     9241 taacgatcaa agtagccaat tcccagaaga aatccatgac gtgtgtatgt ggattgagca
     9301 gatttgcgat aaacgaatag acactataaa tatatgcaac tgtaatcccc atcgcgatca
     9361 gtgtcatcat agcaggactt ttttgtttca attccatctt agctccactt aaaaatggct
     9421 gaccgccata gataaatagt atagttgcta agatcaatac gagccaatca gaaccaggaa
     9481 acgttacttg aaatggaaat tccattccca tcattggtga aaaaagaatg attgggatag
     9541 ctaggataag cgatagccaa aatttttgtt tgaaattccc catgtgcatg gaatgatcca
     9601 tgccgccgtg ttcgtgatcc atgtgcatcg aatggtccat ttgtttgctg tttgctatat
     9661 ctccatggtt atgtcccatg tgcatggaat gatccatctg actatggtca tgtttcatct
     9721 ccatctgttt ttcatgagat tctgtacttt ctttgtttac tacttctttt ttatgctcat
     9781 gttttttctc catatcgttc ccattcattt ttactccatc ccttctctta ctttttcatg
     9841 tgtcgattta agcacaggga gttcaacaat acactgacgg aactaaacgc catcgctcct
     9901 cctgcaatga ttggattcaa aaagcctagt gcggcaaaag gaataccaat cgtattatag
     9961 acaaaagccc agaaaagatt ttgtttgatt tttctcaatg tcaacctaga caactcgatt
    10021 gtttgtgcaa tggatgccaa actgctattc atcagcgtca catcggctgt ttccatcgca
    10081 atgtccgttc cacttcccat tgccataccg acatcagcta atgctaaggc cggtgcatca
    10141 ttgattccat cgcctgccat accgactttt ttgccatctt tctgaagttt ttctacgtag
    10201 gctgctttat cctctggaag tacttcagca aagatatgct ttggatcaat accaacttgt
    10261 ttaccaatcg tttcggctgc cagtttgttg tctcctgtca gcataaaaac atctaatcct
    10321 ttattctgga gctgcttgat tgcttgtttg acttctagtt tgatttgatc agccacggca
    10381 atcaacccga tcacttgctt ttcatcagct aaaaacatga ctgtttttcc ctgctgttct
    10441 aattctaacg cgtgttcttg gtattcatca aatgataggt tcaattcaat taatcgttta
    10501 cgtgtacccg caaaatagcg tactccgtta atcgtaccgc tgattccagc acctgggtgg
    10561 gctgtaaaat caatcatatc gtatgttgct gcttcttgca gttttccgta ttccacgatt
    10621 gctttaccta gtggatgttc agaagcttgt tctaaagtat aaaaaataga taggacttct
    10681 ttagaaccga ctagatctgt aactttgggc tttccttctg taatcgttcc agttttatcc
    10741 aaaacaatgc tgtctaagtg agcagctgct tctaatgctt ctccgccttt aattaaaatt
    10801 ccatttcgtg caccaacacc cgttcctacc ataatagctg ttggagtcgc taaacctaat
    10861 gcacatggac acgcaatgac taatacagag acactatgga tcaatgcgag ttgccaatca
    10921 ccagtgatca aacctgtagc tatgagcgtg ataaacgcga tgactaatac gatcggaaca
    10981 aaaatccctg agatccgatc tgcgattttt tggattgggg ctttgctccc ttgagcatct
    11041 tccaccattt gaatgatttg cgctaatact gtctgcttgc cgacttgtgt aacttccatg
    11101 tgaagtagac cattcgtatt gattgtcccg ccaaagagcg tgtcatccgg attcttttct
    11161 acaggaagat tttcaccagt aagcatactt tcatctatcg cactgcttcc tgaaataatt
    11221 cgaccatctg ctggaatctg ctcaccagga cggatgacta gttgatcacc aacgactact
    11281 tcttcaatcg ctaacgtttc ttctttcccg tttcttatca cttgggcggt ttttgtttga
    11341 agagacatca attgtttgat tgcattacct gttttcgttt ttgctgtatg ttccaaatat
    11401 ttgccgagca aaatcagcgt gatgatcata ctgctgcttt caaaataaag atcttggggg
    11461 tgtccacgga aaaatccgtt gtaaatgctc aatgcaaagg cagccgatgt tccaattgct
    11521 accaatacat ccatatttgg tgctttcgtc ttgattgcat ggtacgcacc tttataaaaa
    11581 cgagcaccga cataaaattg gacaggagca gacagcacta actggacgat tggaaaatga
    11641 aaaaagtgaa caatcgccgc atggcttcct aacatcatag caatcattga caacatcaac
    11701 ggcaaagtca aaaccgtact caatataaga tcgaatagca tttttttcaa atacgcttgc
    11761 ttttcttctg caattttttg tttgtgggct tcatcgtaca aaattgcacc atagccgatg
    11821 ttttctacgc tttggattaa tttttcagtg gtcgtccctt catatttgac acttgctttt
    11881 tcagtggcca agttgactgt ggcattgacc acaccaggtt gtccgttcag ctctttttcg
    11941 atcctagcag aacaatttgc acaagtcata cctgtaatga cgaacgtatc cattttggtt
    12001 tctgctgttg ccatcaaatc acctctgctt tataacccaa tccattgatt gcttgacaaa
    12061 tcttttcgga ttgaactttt gtttcatcaa acttgaccgt accgtttgct tttttcaaat
    12121 ttactttcac tttttgcacg ccgtcaagtg cgcttactgt ttcttctact cttgctgcac
    12181 aatgattgca gctcatacct tcgattgaaa attgttgttt cattttttct tcctcctatt
    12241 gatgttgttt acattcacat tgacctggga tacagttaca ttcgatcgta tctactggtt
    12301 gcttttgttc cagttgtttc aaaatctcat tgatatcttc ttgagtcaaa gtggcttctt
    12361 ctactagatc cgcaatcgtc tctccaatcc tttttgcaca gatatgggaa aaaagatttt
    12421 ccgtcgcact gcgaactgtt tccatctccg agacagctgg ataataaatg aatttttttc
    12481 cttcttgttc tgtccagatc acttcttttt ttaccaaacg acccagcaaa gttttgatcg
    12541 ttgctggttt ccaatccatc gaatcagcta agatctgcgt gatttcttgg gctgtcgtct
    12601 gtccaagtgt ccaaataaca cgcatgatct cccactctga atcactgatc tttactggcg
    12661 ttttgacttt ttccatttat ttcacacctt tcgtttacaa atgtaatcat tatcttatga
    12721 atacagatta cacttgtaaa caaaaactgt caattatttt tgctctctat tttcaattta
    12781 gttaaaaatt cttgtttctc tcgtcttgtt ttaaggatga tcagtttatt tcttgcatta
    12841 gctacttgga tcagctgctg tattttcggt tcattgtttt cttcaaaatg ccagataaaa
    12901 gataaaaatt ctaagtattc cctgtttagt ttctccgaaa atgattcagg catatctggc
    12961 cgagattttt tgtttatctt tctttttaga gaacgccaaa ctactcggta gatggcgaga
    13021 taacgcggca ctttaagcca aatgatctta tctgcttctt taatcctttc ctctaatgta
    13081 gaagtataat ttccttcaac gatccatgac ggattagata ccataaacaa ttgctgttct
    13141 tgcaaaaacc acttttttgc ctgaatactg tagtctgttg tatgccataa aaggtctagc
    13201 ggcagtagcg gaaagtcggt gatttctttg atcttccttg cgagggtcga ttttcccgac
    13261 ccactttgtc caatcacgat gatcttcatt cttctccctc acttctcttt ggaatttggt
    13321 tcgatcatac cacttataac gccgcaaaaa aacaagggac tgtgacaaaa gtcgtgtcaa
    13381 agtcccttgt ttcgaataa
//
LOCUS       DARBET010000070        13281 bp    DNA     linear   BCT 07-NOV-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4027
            SAMEA114569841-rid20112183.denovo.072, whole genome shotgun
            sequence.
ACCESSION   DARBET010000070 DARBET010000000
VERSION     DARBET010000070.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA114569841
            Sequence Read Archive: ERR12203666
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 13281)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13281)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001972618.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001972618.1
            Genome Coverage       :: 132x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/07/2023 13:18:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,041
            CDSs (total)                      :: 2,975
            Genes (coding)                    :: 2,908
            CDSs (with protein)               :: 2,908
            Genes (RNA)                       :: 66
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 67
            CDSs (without protein)            :: 67
            Pseudo Genes (ambiguous residues) :: 0 of 67
            Pseudo Genes (frameshifted)       :: 26 of 67
            Pseudo Genes (incomplete)         :: 28 of 67
            Pseudo Genes (internal stop)      :: 20 of 67
            Pseudo Genes (multiple problems)  :: 6 of 67
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13281
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA114569841-rid20112183.denovo.072"
                     /isolate="VRE4027"
                     /db_xref="taxon:1352"
                     /country="Denmark"
     gene            413..619
                     /locus_tag="SCJ86_002223"
     CDS             413..619
                     /locus_tag="SCJ86_002223"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306600.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3955 domain-containing protein"
                     /protein_id="HEG1169746.1"
                     /translation="MKAIHISLLFFASALILFAIHLCSQPYINNNGILVEPAFFCLPL
                     GFLSLLASAGFGFVAFFKRSKQQI"
     gene            complement(821..1822)
                     /locus_tag="SCJ86_002224"
     CDS             complement(821..1822)
                     /locus_tag="SCJ86_002224"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HEG1169747.1"
                     /translation="MKWFKRIVIAVPVIIVVILVTVFLINQITPKPISLLVRKQFDTS
                     EKEQVYARPDDFQQKIEQIDIKKEISYPSKYKNNNMDIYTPKVEKKKLPILFWMHGGA
                     YVGGDKNDCRDYLEYLCSDTQQIIVNIDYERSPEAKHPTPVIQLNEAIQAAKKEIKDQ
                     ADWSNISIGGNSAGAQISGEYLLALQNEEIKKADHLDPTLENKQITKFISLSGLLDPS
                     ALTQVSDKISSFLYEKCGWAYFDNKDFEQSEKVKSLALVRHADRWTQNTFLTDGNTNT
                     FTKQMEEVTSVFEKAGVKVTKVDYAEKNAVLNHEYQFDFSNKQAQETYQKLVMFFKE"
     gene            complement(1827..3740)
                     /locus_tag="SCJ86_002225"
     CDS             complement(1827..3740)
                     /locus_tag="SCJ86_002225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MucBP domain-containing protein"
                     /protein_id="HEG1169748.1"
                     /translation="MNSLRTKQVLKGALVILFAVSTSVPQWTVYGESISSESESREVE
                     TTETTDAGNETKSSTSQTETNTETTVETSVTSTDQETTATTDNQQTEEATATKMSNDE
                     SSSAKVSDKENGTENDDIGKGENFNGKEYLNYFGKQMTEILNGPTEDIKSKLIELLVT
                     ELAVNDYGVDKEIIKHLIATYLLYDIAFAKNSVLMSMLGGTDDGTTPTENTGFYPNTK
                     QLHQTVLNKQDGKMMDVYAYYVDQGSDKTVMIHGGFRGNWNNGIVTEEYNDFYKAGYN
                     LLFVDSRATGNSGGDYVTYGQYESDDVLYWINQEVRERPSQKILLYGGSMGAATMMSV
                     LAKDIPVNVKGIIENCGFASIDEQLRFTYSQTVAPALPDAIKNQLDIIGDQEHEDLFM
                     GLLKQYYFDQEMHLDTKAALPTIGMSDSLPKLIIHGTADDVVPVSNAQKLYELSGGYK
                     DLLLVEGAGHGKAQEVDHAAYTKHVTDFLKVVFHDQINVKYVDENNKSLLKDQDEIQL
                     YGAYGENYMTEQKTFEGYELANVEGPTEGIFNETIPTIIFYYKKIPVAPPKKQDPTEN
                     ADNKGKNEPREDQSSDDTQPIQMTVKVQKKTDAKKRMLPKTGEKKHSLLIYSGGFLSV
                     STIITWIWRRKRG"
     gene            complement(3908..4414)
                     /locus_tag="SCJ86_002226"
     CDS             complement(3908..4414)
                     /locus_tag="SCJ86_002226"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335735.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine hydrolase"
                     /protein_id="HEG1169749.1"
                     /translation="MSNTADAIVVIDLQKGVCFDSSPIHELETVTALVNKRIKTYETI
                     GKPVIFVQHEDAQLVPQTKPWELLSELSIPEHSYFVRKTHANSFYKTNLSVLLTELDC
                     HSIELCGAQAEYCIDTTVKFAHGLGYQLMMCRGATTTYDNPFMKAKETIAFYEKIWDK
                     RFLTFLEQ"
     gene            4574..5014
                     /locus_tag="SCJ86_002227"
     CDS             4574..5014
                     /locus_tag="SCJ86_002227"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331724.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Spx/MgsR family RNA polymerase-binding
                     regulatory protein"
                     /protein_id="HEG1169750.1"
                     /translation="MMIKLYTGSGCSSSRRAKRWLIVHDIPFIEQNLIRQPLTKNEFI
                     HLLKLTNNGVTEMLSTHSISYKNLVHRIDELSLSELYIEIRTSPSLLKKPIIYDQSAK
                     LLTGFSEDKIRMFLSKTKRKQELNELLLSGHLVDFKENNLFAMS"
     gene            5173..6513
                     /locus_tag="SCJ86_002228"
     CDS             5173..6513
                     /locus_tag="SCJ86_002228"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ISL3 family transposase"
                     /protein_id="HEG1169751.1"
                     /translation="MSYSEFTKELLDILDLNLTFHEDAFRKERINDETCFVFDGTLTY
                     QPEECFHCHYQNKQTIIKWGWKKVSILLNDVSNYKTIVRINKQRFKCKHCGKTFLAED
                     SVSDRRCSIARRVKQAILELLSEPISMSLIARMKHISPTTVIRILRSLRPKTVSLNQP
                     LPEVVCFDEFKSVKNVSGAMSFVMMDGKTHQLIDIVENRQLNPLRDYFLRYPRKVREQ
                     VRLVVSDFYTPYRTLVKECFPNARIVADRFHISQHIGRAFTNHRIQVMKTFKKGDRRS
                     KHLKKYWRLLQKNAWELNGQHRYWRPSFRDHLTEAEIVDRLLYYDDSLKRGYEVYQVF
                     LSAIRRQDVPEFVALLKEDYKELPEHYQPVFTTFKKYRTEIKRALRVPYSNGPIECLN
                     NHIKVLKRIAYGFRNFQNYRERIFLYRGKYFKKTKNTTQLTKARTTTRLDKIAV"
     gene            complement(6582..7739)
                     /locus_tag="SCJ86_002229"
     CDS             complement(6582..7739)
                     /locus_tag="SCJ86_002229"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296632.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HEG1169752.1"
                     /translation="MRTIQRKLRELNTILKNYHLSCSLKKGNPIEGEEYRIRYFFHLM
                     YWQSFDENHSDILSSIKNKRQLLEKFAKHAPYTRSIDRKKWISLLRISLQRMKQGHSI
                     TYVPEEMNKFVHPLITKEEFTHYILEPFFEANFWSMNHITPTEQAYLYFMFGVMNTYL
                     EEDLLDTPDKVQESWGWIESSIVENFEKNFKICFTTSEKNYLYVNLAMIHLYSRVFGT
                     KKKVDAFGKSAEDRDFQHIFPVMYPIMQQFFSGVARRIPELNTYYEKNRRLIFQYCML
                     VRDIFIKHEQPVIFYIQSKYGKTQELWAKKRILSMTERPIKYSASADQLPDIVISDYP
                     IDKKAYDQTNTQIFYWNGQPTKNDWARLKAEIIKIRNEKNRSILEKLDFVN"
     gene            complement(7742..8098)
                     /locus_tag="SCJ86_002230"
     CDS             complement(7742..8098)
                     /locus_tag="SCJ86_002230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296631.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEG1169753.1"
                     /translation="MFEEFIDINERQVYQFLNYCYERDEKLYVVKDIALDLNYTLAKM
                     NSVIQQAESFCERYPEYKLSFLSENKMIKVEFSSQFLLSKVYSILLEGTIGYILLDSL
                     YKGTYQSLENLSQKII"
     gene            complement(8396..9370)
                     /gene="sgrA"
                     /locus_tag="SCJ86_002231"
     CDS             complement(8396..9370)
                     /gene="sgrA"
                     /locus_tag="SCJ86_002231"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LPXTG-anchored fibrinogen/nidogen-binding
                     adhesin SgrA"
                     /protein_id="HEG1169754.1"
                     /translation="MKKTATVLFVILGLSIIAAMIGISTSANERANENLSFTVKTDRI
                     VYDMTQQVITIPVKPNKSVNASDVHAVLTYGWDGNGSSEKVIGEVYLKDVQWTAGIEY
                     TIMISAELSIDEIKSKDKVDLIVFYDGQMTITENLKPSSWTVVGPPSSTDSTSSESST
                     ENTSGESSTESTSSESSTENTSSESSTESTSSESSTGSTSSESSTESTSSESSTGSMS
                     SESSTGSTSSESSTGKVNNELDLVPIIPTKEQKRNAGSNLKSVLKQPDFVSRGIDHQE
                     KAKETDNRKSDLPETGSRTLNKLITWMGVLLILIVGASYFRSLFHRVK"
     gene            complement(9566..10405)
                     /locus_tag="SCJ86_002232"
     CDS             complement(9566..10405)
                     /locus_tag="SCJ86_002232"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002378019.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CPBP family intramembrane metalloprotease"
                     /protein_id="HEG1169755.1"
                     /translation="MIRIIRLIGSVLIVLVGFVLGILVNNALADMFIGNSEWIGAIAG
                     TVGHLAVFLLALFLASKVEGKRIEDYGVSVRGKDWGYLAGGLVVGIGVFLLITSPLYL
                     TGAYRLDSGNANIVPLITSFILFIAVGASEELLFRGFFQHQMLRFGPLIAMIGSAALF
                     ALLHGLNPNMTFLAVFNIFLAGCFFSALIYSTGSLFTAIGAHITWNWTQGAILGIPVS
                     GTQEQGFFSTLIQSQNTIITGGNFGAEASISTTLFLLVLIVGLLLYAYKTKRMKKYTA
                     VYQ"
     gene            10590..11477
                     /locus_tag="SCJ86_002233"
     CDS             10590..11477
                     /locus_tag="SCJ86_002233"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidyl-prolyl cis-trans isomerase"
                     /protein_id="HEG1169756.1"
                     /translation="MGNFLKKQLLIMCLSACAILLFSGCSKSNETIATFKGGTLTVQD
                     FYDNPKVKSNNQTLLKKMIVFKAFDDVYGKEISSKEVTKEYNEQIKKLGPNYKEQLKA
                     VGQTEETYKKLFKQMLAFQYGLKANVKLTDKDLDTAWKEFYPEVSTQIILFSTKEEAD
                     KAKKEANEGENFSKLVQAYGKNKTLKETDGKMNFDSTNPEIPTEVKKAAFKLKNGEVS
                     DIIPVTDPTTYQQSYYLVKMVKKQDKGSNKDKYKSELEKIATEAKTLDTEFMDKTIRK
                     VMKKDNVTIKDPYVKNIFK"
     gene            complement(12061..12756)
                     /locus_tag="SCJ86_002234"
     CDS             complement(12061..12756)
                     /locus_tag="SCJ86_002234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628000.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fructose-6-phosphate aldolase"
                     /protein_id="HEG1169757.1"
                     /translation="MEIMLDTANTKSIYSCQQFLNLTGVTTNPTILKKEGASNFFSHM
                     KEIKKIIGQIPIHIQTIGRTEEEMIEDARVIIEELGKDTFIKVPVNQAGLQAIKKLKK
                     EGYLITGTAIYTELQGYLAINNGADYIAPYYNRMQNQGIDAMKVIDSLRTEIERTQRP
                     TKILAASFKNCEQVTTAIRSGAHAITLGKELLDQVLEMPSIDKAVRGFQEDWVQTFQA
                     ETIGELKKVADYF"
     gene            complement(12740..13138)
                     /locus_tag="SCJ86_002235"
     CDS             complement(12740..13138)
                     /locus_tag="SCJ86_002235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628001.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucitol/sorbitol transporter subunit IIA"
                     /protein_id="HEG1169758.1"
                     /translation="MTIFETVVTDVGIEAEVFRKEKIMILFGDEAPETLTDYCYKIEL
                     NQVRKEITCDMKLCFDKQMYVITAVGDVVQTNLNELGHITIKFDGSHEPELPGTMYVE
                     DKEMPHLRKGTIIKIDQEDQNGGKLYGNHA"
BASE COUNT         3979 a         2665 c         2055 g         4582 t
ORIGIN      
        1 tatttttgtt tcttattttc taaaaaatta aaaaaaattt tggtgaatta agtatttcct
       61 tgataacact ggcttttggc ctcattttag gttatcattt gtaattttat ttcacagttg
      121 atatgattgt tatgtaaaat aaaaaagttt aggatacatc gaatttttgg ggaaattctt
      181 ttgggggtat cctaaatctt ttctattata ttcccatatt ttctattatt ggtctagacg
      241 tatgatggta aactttgtac ctatctgatt gctgaatcat taattgctta tcattagcca
      301 tctttgtttc aagttcgcta gatgatcatt ttattcacat cctaacaaac ttgtcacatt
      361 tatagattct taatctattt atcatacaat tataaaaaag gaggtgctgc ttatgaaagc
      421 tatccatatt tctttgcttt ttttcgctag tgcattgatt ttatttgcta ttcatctttg
      481 ttcccagcct tatatcaata acaacggaat attagtggaa ccagcattct tttgcttacc
      541 actcggcttt ttgtcccttc ttgcctccgc aggcttcgga tttgttgctt tttttaaacg
      601 atcaaaacaa caaatctgac ttataaaatt tacgtaaaag agatcgtgac aataatcgtg
      661 tgatattccc aaatttcgaa taaaaggtgc cccttgccat ctctaactcc tcagtcacaa
      721 gttacaatat tcataaacca tgaaaagctt gtttattgaa attgctcctt gtgacttgga
      781 gctaagtgac agattacaac ctcttttgca tcataagact ttactccttg aaaaacatta
      841 ctaacttttg atatgtttct tgcgcttgtt tattgctgaa atcaaactga tattcatggt
      901 tcaaaacagc atttttctcc gcataatcta cttttgtgac tttcaccccc gctttctcaa
      961 atacagaagt tacttcttcc atttgtttcg taaacgtatt tgtatttcca tccgttaaga
     1021 atgtgttttg cgtccaacga tccgcatgtc tgaccaaggc taaacttttg accttctctg
     1081 attgttcaaa gtccttatta tcaaagtaag cccatccaca tttttcataa agaaagctag
     1141 agattttatc acttacttga gtaagtgcag aaggatccaa cagaccagac aaagaaataa
     1201 acttcgtaat ctgcttgttc tccaaagtcg gatcaagatg atcagctttt ttaatttctt
     1261 cattttgaag tgctaacaaa tattctccac taatttgtgc acctgccgaa tttccaccaa
     1321 tcgaaatatt cgaccaatct gcttgatctt ttatctcttt tttagctgct tgaatggctt
     1381 cgttcaattg gataactggt gtaggatgtt ttgcttccgg tgatcgttca taatctatat
     1441 ttacgatgat ttgctgcgtg tctgaacata gatattccaa ataatcacga caatcatttt
     1501 tatcccctcc tacatatgcg cctccatgca tccagaaaag aatcggtaat tttttctttt
     1561 ccactttcgg tgtgtagata tccatattat tatttttgta ctttgaagga taggatattt
     1621 ctttttttat atcgatttgt tcgatttttt gctggaaatc atctggtcgg gcatatactt
     1681 gttctttttc acttgtgtca aattgttttc taacaagcaa gctaatcggc tttggtgtga
     1741 tttgattaat caaaaataca gtgactaaaa taactacgat gataactggt actgcaatca
     1801 ctattcgctt gaaccatttc attcatttac cctctctttc ttcgccaaat ccatgtgata
     1861 atcgtgctaa cagataaaaa tccacctgaa taaatgagaa gcgaatgttt cttttctcca
     1921 gttttcggca acattctttt ttttgcatct gtcttttttt gtactttcac tgtcatctga
     1981 atcggttgcg tatcatcact tgattgatcc tctcgaggtt cattctttcc cttattgtct
     2041 gcattctctg tcggatcttg cttttttggt ggagctactg ggattttttt gtaatagaaa
     2101 ataatagttg gtatcgtctc attaaaaatc ccttcagtcg gtccttcaac atttgctaat
     2161 tcgtatcctt cgaatgtctt ttgctctgtc atataatttt ctccataagc tccgtaaagc
     2221 tggatttcgt cctggtcttt gagtagactt ttattgtttt catccacata ctttacgttt
     2281 atctgatcat ggaacactac tttcaaaaag tccgttacat gtttcgtgta ggcagcatgg
     2341 tcaacttcct gtgcctttcc atgccctgct ccttctacta ataaaagatc tttataccct
     2401 cctgataact catataattt ttgcgcgtta gacacaggta ctacatcatc tgctgttcca
     2461 tggataatca atttaggcaa tgaatctgac attccaatcg taggtagagc agcttttgta
     2521 tccaaatgca tctcctgatc aaaatagtat tgtttcaaaa ggcccataaa taaatcttca
     2581 tgctcttgat ccccaatgat gtctaactga ttttttatgg catccggtag tgcgggggct
     2641 actgtctggc tgtacgtaaa acgtagttgt tcatcaattg atgcaaatcc gcaattttca
     2701 ataattcctt tgacattgac aggaatatct ttcgctaata cagacatcat cgtagctgct
     2761 cccatcgaac caccataaag taaaatcttt tgagatggcc gctctcgaac ttcttgattt
     2821 atccagtaaa ggacgtcatc tgactcatat tgtccatagg tcacataatc gcctccgcta
     2881 ttaccagtcg ctctggaatc aacaaataat aaattatacc cggccttata aaaatcattg
     2941 tattcttctg taacgatccc gttattccaa ttccctcgaa atccgccatg gatcatgact
     3001 gttttatctg aaccttgatc gacataatat gcatatacat ccatcatttt gccatcttgt
     3061 ttgtttagta cagtttgatg aagttgtttt gtatttggat agaagcctgt gttttctgta
     3121 ggcgttgtcc catcatctgt acctccaagc atcgacatca acacactgtt ttttgcaaaa
     3181 gcgatatcgt acaatagata agtagcaatc agatgtttga taatctcttt atctacacca
     3241 taatcattaa cagcaagctc agtgactaat aactctatta attttgactt gatgtcttct
     3301 gttggtccat taagtatttc tgtcatctgt ttaccgaagt aattcaaata ttctttacca
     3361 ttgaaatttt cacctttacc gatatcatca ttttctgtcc cattctcttt gtcacttact
     3421 ttagcagaag aggattcgtc gtttgacatt tttgtagctg ttgcttcttc tgtttgttgg
     3481 ttatctgtgg tagcggtcgt ttcttgatct gtgctagtaa cagatgtctc aaccgttgtt
     3541 tcagtatttg tttctgtttg tgaagtactc gatttcgttt catttccagc atcagtagtt
     3601 tctgtggttt ctacctcccg agattccgat tcggaagaaa tgctctctcc atatactgtc
     3661 cattgcggga cgcttgtact tacagcaaat aagatgacca acgctccctt taatacttgt
     3721 tttgttctca agctattcac tcctttttat gtttctagga gaattatatg taaacgcaaa
     3781 cattttttat acaaaaaata aatggtttat cccataaaaa tagaacagga ttattttctc
     3841 attttacgta ataaagcaga atggataaac cagtatccat tctgcttttt ttaaacgaag
     3901 aaacaactta ttgctccaaa aatgtcagaa atcgtttatc ccaaattttc tcgtaaaaag
     3961 cgattgtttc ctttgctttc ataaaaggat tatcataagt tgtcgttgct cctctacaca
     4021 tcataagctg gtatcctaat ccatgagcaa acttcacagt cgtatctata caatactctg
     4081 cttgtgcccc acataattcg atcgaatgac agtccaattc agttaataaa acactcaaat
     4141 tagtcttata aaatgaattc gcatgggttt ttctcacaaa ataactgtgc tcaggaatag
     4201 ataattccga caatagctcc catggttttg tttgtggcac taattgagca tcttcgtgtt
     4261 gaacgaaaat aactggtttc ccaatcgttt cgtatgtttt gattctttta ttcactaatg
     4321 ctgtcactgt ttctaattcg tgtatcggcg aagaatcaaa acatacccct ttttgtaaat
     4381 cgatcactac tattgcgtcg gctgtgtttg acatacttgt gccttctttc ttttttcttg
     4441 atttaatagt atttcttatc tagctagttc cattatacgt gacaacattt tcttatttta
     4501 cacctctaaa tggcactttt cgactatttc ttcatgttat tatttcataa atacttagct
     4561 attggaggaa tgtatgatga ttaaattgta tacaggttct ggctgctcgt cttcaagacg
     4621 cgcaaaacgt tggcttatcg tccatgatat accgtttatc gaacagaact taattcgtca
     4681 accactaacc aaaaatgaat ttatccactt actaaaattg acaaacaacg gcgtgaccga
     4741 aatgctctct actcattcta ttagttataa aaatcttgtt cacagaatag atgaactcag
     4801 tttgagtgaa ctatatattg agattcgtac atccccttct ttattgaaaa aaccgattat
     4861 ctacgaccaa tctgcaaaac ttttgactgg atttagcgaa gataaaatca ggatgttcct
     4921 ttctaaaact aaaagaaagc aagagctcaa cgaactcttg ctttctggtc atcttgtcga
     4981 ttttaaagaa aataatcttt ttgcaatgtc ttaattttgg cataaacaat ctgcttttct
     5041 ggatctataa tatctagtgt tggtgacgcc ttctgttgcc aatacttttt cttttttcta
     5101 ataaacgcaa acagacacct tgaaatcctt tagaataaag tcgacgaaaa ccattcaaag
     5161 gagtgtttca agatgtccta ttcagagttt accaaagagt tactagatat tctagaccta
     5221 aatcttacct ttcatgaaga tgcctttcgc aaagagcgca ttaatgacga aacatgtttt
     5281 gtttttgatg gcacattgac ctatcaacca gaggaatgct tccattgcca ctatcaaaat
     5341 aagcaaacga tcattaaatg gggctggaaa aaagtatcca tcttattgaa tgatgtcagt
     5401 aactacaaga ccattgtgcg gattaacaaa cagcgcttca aatgtaaaca ctgtggaaag
     5461 acttttttag cagaagattc agttagcgat cgtcgctgct ccattgcccg cagagtcaaa
     5521 caagccattc ttgagctatt aagtgagcct atttctatgt ctttgattgc acgtatgaag
     5581 cacatttctc cgaccactgt cattcgaata cttcgcagcc ttcggcctaa aaccgtttct
     5641 ttgaaccagc cgttaccaga agtggtgtgt ttcgatgaat tcaaatcggt gaaaaacgtc
     5701 tcgggagcca tgagctttgt catgatggac ggcaagaccc atcaactgat tgatattgtg
     5761 gaaaatcggc aactcaatcc tttacgcgac tatttcttgc gctaccctag aaaagtccgt
     5821 gaacaagtac gtctagtggt ttcagatttc tatacaccct atcgcacatt ggtgaaagag
     5881 tgttttccga atgcccgaat cgtcgccgat cgctttcata ttagccaaca catcggtcgg
     5941 gcctttacca accaccgaat tcaagtaatg aagaccttta aaaaagggga tcgtcggtcg
     6001 aagcacttaa agaaatactg gagactactt caaaagaatg cgtgggaatt gaatggtcag
     6061 catcgctact ggcgtccgtc atttcgagat catctgacag aagcggagat agtcgatcgg
     6121 ctattatatt atgatgactc cttaaagcga ggctacgaag tctaccaggt ttttctttcc
     6181 gctatcagaa gacaagatgt tcctgaattc gtcgcgctcc taaaagaaga ctacaaggag
     6241 ttgccagagc actatcaacc agtgttcacg accttcaaga aatatcgaac agaaatcaaa
     6301 cgagcgttgc gagttcccta ttccaacggc cctatagaat gtttgaacaa ccatatcaaa
     6361 gtactaaaac gaattgccta tggctttaga aactttcaaa actaccgaga acgaatcttt
     6421 ttatatcgag gaaaatattt caagaaaact aagaacacta cccaactgac taaagccaga
     6481 acaacaacac ggctagacaa gatagctgtc tagccgtgtg agtatctcga ttattctatt
     6541 gaatttcttc accaacactc attgacgaag agccgctttt cttaattaac gaaatccaat
     6601 ttttccaata tggatctatt tttctcattc ctgattttta tgatttcagc tttcagacgc
     6661 gcccaatcat tttttgtagg ttgtccattc cagtaaaata tctgtgtatt cgtttgatca
     6721 tatgcctttt tgtcgatagg ataatcgcta atgacaatat caggtaactg atcagcggat
     6781 gcactatatt ttattgggcg ttcagtcata gataatatcc ttttctttgc ccataattcc
     6841 tgtgttttac catactttga ttgaatatag aatatgacag gttgttcgtg ttttataaaa
     6901 atatccctga ctaacataca gtattgaaaa atcaaccgtc tgttcttttc ataataagta
     6961 ttaagctctg gaattctgcg agctacccca ctaaaaaact gttgcatgat tggatacatc
     7021 actggaaata tatgctgaaa atctctgtct tctgctgatt ttccaaaagc atccaccttc
     7081 ttttttgtcc caaaaaccct tgaatacaaa tggatcattg ccaaattgac atatagataa
     7141 tttttttctg aagttgtaaa acaaatcttg aagttttttt caaaattttc tacaatagac
     7201 gattcaatcc agccccaact ttcttgtact ttatctggag tatccaacaa atcttcttcc
     7261 aaataggtgt tcattacacc aaacataaaa taaaggtaag cttgctctgt tggagtgatg
     7321 tgattcatcg accagaagtt tgcttcaaaa aaaggctcta gtatgtaatg cgtaaactct
     7381 tcttttgtta tcaatggatg cacaaatttg ttcatctctt ctgggacata cgtaatcgag
     7441 tggccttgtt tcattcgttg caacgaaatt cttagtaggc tgatccattt ctttctatct
     7501 atactacgcg tatatggcgc atgtttcgca aatttttcta atagttgacg tttatttttt
     7561 atacttgata atatatcaga atgattttcg tcgaatgact gccaatacat caagtgaaaa
     7621 aagtatcgga tacggtattc ttctccttct atcggattac cttttttcaa gctgcaagat
     7681 aaatgatagt ttttcaaaat agtattaagt tctcttaatt ttctttgaat cgtacgcaaa
     7741 cttatataat tttttgacta agattttcta aggattgata agtacctttg taaagtgaat
     7801 ccagtaaaat ataaccaatc gttccctcta aaagtataga ataaacttta ctcagtaaaa
     7861 actgagaaga aaactcgact tttatcatct tgttttctga aagaaaagat aatttatatt
     7921 ctggatacct ttcgcaaaac gattctgctt gttgaatcac cgaattcatc ttagccaggg
     7981 tgtagtttag atctaaagcg atatccttca ccacatataa tttctcgtct ctttcataac
     8041 aatagttcaa aaattgatat acttgccttt cattgatatc aataaattct tcaaacaaag
     8101 tacatactcc tttttatcta ttatttagac ttattccgac agctacatga ataacgtaca
     8161 aatagattcg ttactgattt ttcagagata ctaccatgcg cagtgtgcat tgtcaattat
     8221 tcttgctctt attttccatt tgtgttcctg ttatttcctg tttctcaaat atgttagtaa
     8281 acaaaaatag atctggcaaa gctacaactt cgccaggcaa tacagcactc tcgaatgaaa
     8341 tattcactca aaaatctatc gttttattca attttagatc tgttgctttt attctttatt
     8401 tcactcgatg gaagagagaa cgaaaatagc ttgctcctac aatcaaaatg agtaacacac
     8461 ccatccatgt aattagcttg ttcaaggttc tactaccagt ttctggtaaa tcgctttttc
     8521 tattatctgt ttctttcgct ttttcttgat gatcaattcc acgagaaaca aaatcaggct
     8581 gttttagcac actttttagg ttgctccctg catttctctt ttgttcctta gttggtatga
     8641 taggtaccaa atcaagttca ttatttactt ttcctgtgct gctttcgctg ctcgtgcttc
     8701 ctgtgctact ctcactactc atgcttcctg tgctactttc actactcgta ctctccgtac
     8761 tgctttcgct gctcgtgctt cctgtgctac tttcactact cgtactctcc gtactgcttt
     8821 cgctgctcgt gttttctgtg ctactttcac tgctcgtact ctctgtactg ctttcaccgc
     8881 tcgtgttttc tgtactgctt tcactacttg tgctatccgt actgcttggc ggtccaacaa
     8941 ctgtccaact gcttggtttc aaattctccg tgatcgtcat ctgtccatca taaaaaacaa
     9001 tcaaatcaac tttatcttta ctcttgatct catcaatcga tagttctgca gaaatcatta
     9061 ttgtatactc aataccagcg gtccattgta cgtcttttaa ataaacttcc ccaataactt
     9121 tttctgaact tccattacca tcccaaccat atgttagaac agcatgtaca tcactagcat
     9181 tcacgctttt gtttggtttg actggaatag taatgacttg ttgtgtcatg tcataaacaa
     9241 tccgatcagt tttcacagta aatgaaagat tctcatttgc ccgttcattt gcagaagtac
     9301 tgattccaat cattgctgca attatactca gcccaagaat aacaaataat acggtcgctg
     9361 ttttcttcat ttctatcact ctccatataa ttaccttcta tttgataaag gaattatata
     9421 aaatgaaaat gatgagaaca cgacagtcag ttgtgaaaat ctaacaaaag atgtcgtgta
     9481 ttcgcttgaa tttgtttact ttttcgttta atcaatgaca gacttaaaaa agcaagaccg
     9541 atatcagcct tgctttcttc atcttttatt gataaacagc agtgtatttc ttcatccgct
     9601 ttgttttata tgcatagagt aacaaaccaa caatcaagac taacaagaat aatgtagttg
     9661 atatagatgc ttcagcccca aagtttcccc cagtaattat tgtattttgc gattggatca
     9721 gagtggagaa aaaaccttgt tcttgcgttc cgctgactgg tatacccaaa atagctcctt
     9781 gtgtccaatt ccaagtaata tgagcaccaa tagccgtaaa taaacttcct gtactgtaga
     9841 taagtgcgct gaaaaagcaa cctgctaaaa agatattgaa aactgccaga aatgtcatgt
     9901 taggattcaa tccgtgcaaa agagcaaata atgcagcaga tccaatcatt gctattaacg
     9961 gaccaaatct aagcatctga tgctgaaaaa agccacggaa taaaagctct tctgatgccc
    10021 ccactgcaat aaataaaata aaactcgtta tcagcggcac aatgtttgcg tttcctgaat
    10081 ctaaccgata cgcccccgtc aaatataatg gactagtaat aagcagaaac actccaattc
    10141 caacaacgag acctcccgct aaataccccc agtcttttcc tcgaacagaa acaccataat
    10201 cttctattct ttttccttcg accttactag caagaaataa agctagtaaa aaaacagcta
    10261 aatgtccgac cgtaccggca attgctccaa tccattcact atttccaata aacatatccg
    10321 caagtgcatt attgacaaga attcctaaaa caaaaccaac taagactatt aaaacagatc
    10381 caatcaaccg aataatcctg atcatctttc atccctcctt tatattaagt ttaacaaccc
    10441 ttttctttct tgtctcgtga aaactatgta aatgttctcc atcatagaga gaatagattt
    10501 ttacgaacta agaatatcta cagcaacctt acgaaaatga aagactgtct ccctaattct
    10561 tctgttagac tgtttttaat aaggaaagga tgggaaattt tttgaaaaaa caactactaa
    10621 ttatgtgcct ttcagcatgt gcaattttac tattctcagg ttgtagcaag tccaatgaaa
    10681 cgattgcaac gtttaaaggt ggaacactta cagttcagga tttttatgat aacccaaaag
    10741 ttaaatcaaa taatcaaaca ctattaaaaa aaatgattgt cttcaaagca tttgatgatg
    10801 tatacggaaa agagatttct tcaaaggaag taacaaaaga atataatgaa caaatcaaaa
    10861 aactcggtcc aaactacaag gaacaattaa aagcagttgg ccaaacagaa gagacatata
    10921 agaaactctt taaacagatg ctggctttcc aatacggttt gaaagccaat gtaaaattaa
    10981 cagataaaga tctagacact gcttggaaag aattctatcc agaagttagt acacagatca
    11041 ttctattttc gactaaagaa gaagcagata aagcaaaaaa agaagcaaat gaaggggaaa
    11101 atttctccaa gcttgttcaa gcttatggaa aaaataaaac attaaaggaa actgatggaa
    11161 aaatgaattt tgattcaacc aatccagaga ttcctactga agtaaaaaaa gcagcattta
    11221 aactaaaaaa cggggaagtt tctgatatca ttcctgtaac agatccaacg acttatcagc
    11281 aaagctatta tctagtaaaa atggtaaaaa aacaggataa aggttctaat aaagacaaat
    11341 acaaatctga gctagaaaaa attgctaccg aagcaaaaac tcttgatacg gaattcatgg
    11401 ataaaacaat ccgtaaagtg atgaaaaaag acaatgtaac tattaaagat ccttatgtaa
    11461 aaaatatatt caaataaaca aatttgaccc aattggggat gtggcaaaat ttgagaagtg
    11521 atttttggaa attccttctt atttttgagg caaaccactt ttgccacaac ttcatcgggg
    11581 ccttttttta tttgtaccaa aacaaatcac tggatttgtt acaatcatga ggatataatt
    11641 taaaaaagtt ttttatttct atcacatgaa tcaatgcgcc attaagtaga ttctttcatg
    11701 cttccctatt ttttcacttg ccgtatcgct tcaaaaacaa ctatcataaa ctcaaactcg
    11761 actgaaaagt tattttaaaa aagactaaaa atatcccttc tggacttgtt cctcttattc
    11821 tgttatacta gagaaacacg aaagatattt ttatgcgaca cttttcattt gtttttatga
    11881 aatttattca aaactggcgt aacttcagag ttacaaggat gtattagagg ttagggaata
    11941 catcgtttaa atgagaaata agtaatagtc acccaaactt taaattaatg aatattgaat
    12001 cgtcagatag cctcctcgag ttttcgagaa ggctatcgtt tattttatgg agaaattgat
    12061 tcagaagtaa tcagcgacct tttttagttc accaatcgtt tctgcttgga aagtttgcac
    12121 ccaatcttct tggaagcccc tcactgcttt atcaatcgag ggcatctcta atacttgatc
    12181 tagtagctct ttacctaacg ttatcgcatg agctccagat cgaatggctg tagtgacttg
    12241 ttcacaattt ttgaaactag ctgctaatat ttttgttggt ctttgcgtac gttcgatctc
    12301 agtccgcaac gaatcaatca ctttcatagc atcaatgcct tgattttgca tcctgttata
    12361 ataaggtgca atataatctg ctccattatt aatagctaaa tacccttgta attctgtgta
    12421 aatagctgta ccggtaatca aataaccttc ttttttcaac tttttgatcg cttgaaggcc
    12481 tgcttgattc accggcacct tgataaacgt atcttttccc agttcttcta tgataactcg
    12541 agcatcctct atcatttctt cttccgttct tcctattgtc tgaatatgga taggaatctg
    12601 tccgattatt tttttgattt ctttcatatg cgagaagaag tttgacgctc cttctttttt
    12661 cagaattgta ggatttgttg ttacgcctgt taaattcaaa aactgttgac aagaataaat
    12721 actttttgta tttgctgtat caagcatgat ttccatatag ctttccccca ttctgatcct
    12781 cttgatcgat tttaatgatt gtcccctttc ttaaatgcgg catttcctta tcttctacat
    12841 acattgttcc tggaagttcc ggctcatgag atccatcaaa ttttatcgtg atatgtccta
    12901 attcattcaa attagtttgg acaacatctc ctactgctgt gattacatac atttgtttat
    12961 caaaacagag cttcatatca caagttattt cttttcttac ctgattcaat tcaatcttat
    13021 aacaataatc tgttaatgtt tccggtgctt catctccaaa taaaatcatt attttttctt
    13081 tcctgaatac ttctgcttct ataccaacat ctgttacaac cgtttcaaag atcgtcatct
    13141 ctgttcctcc tcgtaaaata tcatttgtct aatagttttt attcaaaatg tttctagatt
    13201 ataaaattta ttgaataaca cattcctaac ggaatttgtt tcgatattgt gtgatttctc
    13261 agagtacaat ggatgtatgg a
//
LOCUS       DARBET010000071        13260 bp    DNA     linear   BCT 07-NOV-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4027
            SAMEA114569841-rid20112183.denovo.073, whole genome shotgun
            sequence.
ACCESSION   DARBET010000071 DARBET010000000
VERSION     DARBET010000071.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA114569841
            Sequence Read Archive: ERR12203666
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 13260)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13260)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001972618.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001972618.1
            Genome Coverage       :: 132x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/07/2023 13:18:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,041
            CDSs (total)                      :: 2,975
            Genes (coding)                    :: 2,908
            CDSs (with protein)               :: 2,908
            Genes (RNA)                       :: 66
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 67
            CDSs (without protein)            :: 67
            Pseudo Genes (ambiguous residues) :: 0 of 67
            Pseudo Genes (frameshifted)       :: 26 of 67
            Pseudo Genes (incomplete)         :: 28 of 67
            Pseudo Genes (internal stop)      :: 20 of 67
            Pseudo Genes (multiple problems)  :: 6 of 67
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13260
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA114569841-rid20112183.denovo.073"
                     /isolate="VRE4027"
                     /db_xref="taxon:1352"
                     /country="Denmark"
     gene            166..945
                     /locus_tag="SCJ86_002236"
     CDS             166..945
                     /locus_tag="SCJ86_002236"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEG1169759.1"
                     /translation="MEKPFVKSLTAVAVGAGAIAICLFGYHINNQRQHQQRINYAESA
                     ITNQKDTLTSLSKEVDKLYSTKEKIFLNPEITEETITSLSNKLSSVKLSADDYDIKES
                     ELPKEAVAIQDEKKAIVTQLDSAESKLKIQTAVNDLFTKNVSNWQQAVDDVIIKEKVT
                     SADVARVRENMSFFKDSPWKTVVMQYLGFADIQIAQVTQLDQLFDTMLKDGQVTATAT
                     YDQYLTVLSQIEQIRNEKISAAYATKAQTVAEQMGYSNISY"
     gene            991..1935
                     /locus_tag="SCJ86_002237"
     CDS             991..1935
                     /locus_tag="SCJ86_002237"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LCP family protein"
                     /protein_id="HEG1169760.1"
                     /translation="MARRRRKSRGKKIFLIILAVLIVLVLAIVGVGAKLYLDLSHSIE
                     KTYESVERTQGERTKPVDLDKQESFSVLLMGIDTGDLGRVDQGRSDTMMVATVSPEDK
                     QTTIVSIPRDTYVDIVGRDTKDKINHAYAFGGAAMAMDTVQKYVDIPIDHYVSINMKG
                     LKELVDAVGGIEVNNDLTFSQDGYDFPIGKITLNGDQALSYSRMRYEDPNGDYGRQGR
                     QRKIVEGVAKKVLSLNGVSNYQSVLNALESNMKTDMNFDEMKKIALDYREAFGTIKQD
                     QLQGEGFMQDNISYQKVSDQELDRVKKELKDQMNLENK"
     gene            1951..2730
                     /locus_tag="SCJ86_002238"
     CDS             1951..2730
                     /locus_tag="SCJ86_002238"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine protein kinase"
                     /protein_id="HEG1169761.1"
                     /translation="MEETISLKEIFETLRKHLVIIIISMFAGLAISGIVTFFILTPKY
                     SSQAQLIVTLPQSDTANVNDVNTNLQMINTYKDMIVSDLVLNEVKDRLETEDNVKMTA
                     EQIKDAISVNQAENSQMFSIQAISTNAVAAQQIANTTSQVFQENAKDVMNVDKISIIS
                     GAVANTTPVSPNNKLNLMTGLMLGMMVGVGLAFLLELLDRTVKDEKYVTDTLGFPILG
                     TVPEMSAKELNAGIRKKGPAQTQPKATNDKTAPSRRSRSRV"
     gene            2742..3440
                     /locus_tag="SCJ86_002239"
     CDS             2742..3440
                     /locus_tag="SCJ86_002239"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346049.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CpsD/CapB family tyrosine-protein kinase"
                     /protein_id="HEG1169762.1"
                     /translation="MARTQKQKAAQTHAVSLVTLTNPSSPIAEQYRTIRTNIQFASAA
                     GQQIKTIVVTSSGPGEGKSTTAANIAVVFAKSGQRVLLVDADLRKPVIYKTFKLNNAS
                     GLSTVLSSSGSVADEIQRTPVDNLSILPSGPKPPNPSELLSSPRMDQILAEARQLFDV
                     VIFDMPPVVAVTDAQIMSSKTDGTLLVVRENTSRKESLTKAKELLDMVQARVLGVVYN
                     GAEHSKDAGYYYGN"
     gene            3468..4232
                     /locus_tag="SCJ86_002240"
     CDS             3468..4232
                     /locus_tag="SCJ86_002240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738108.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine protein phosphatase"
                     /protein_id="HEG1169763.1"
                     /translation="MIDLHCHILPGIDDGAENLEASIAMAEKAIQQGITHILCTPHHN
                     NGKYSNEKSQVISLVASLQAELEKRQLPLTLLEGQEVRITGTLIEDIHRDEILFTDLD
                     DTYLLIEFPTLEVPLYAERLFFELLELGKIPVIVHPERNAHFREDPNHLLPFLDMGCL
                     AQLTAPSYVGVFGKDIQKTAKTMVKHNLVQMAASDAHGVSKRTFYLKECYEQIAKEFG
                     KEKVVQMKQVAKDLINGDEINYPIYEEVKKKKFGLF"
     gene            4276..5727
                     /locus_tag="SCJ86_002241"
     CDS             4276..5727
                     /locus_tag="SCJ86_002241"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343627.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar transferase"
                     /protein_id="HEG1169764.1"
                     /translation="MYRKNSSGWSKHIDFILLDLFCVQLAFYISYVMRQGDWNPYVIP
                     IYRHMAIFIELENLTIIFLFEAYKNVLKRGYYKEAVASIKQSFMLLLVCSLYLITMQD
                     GNDYSRVSLFIMGVVYGLLTYIIRILWKGLLHHRMKNGEDVSLVIVTSQKIAKNVVRN
                     LKEKNYGMYKLSGVIVVDRDMYGTTIGGVPVVANADNAAEYLLQNWVDEVFINVDESV
                     PYPKELIARCSEMGLAVHMNLTKVSDSTRGKQFIGRVGDYTVITSSINVMTMRQAFIK
                     RTIDILAGIIGCIATGVIFIFVAPVIYIKSPGPIFFAQERIGQNGKTFKMYKFRSMYM
                     DAEERKAELMKENKMSNNLMFKMDFDPRIIGNKILPDGTKKTGIGQFIRSTSLDEFPQ
                     FYNVLMGKMSLVGFRPCLKSEYEKYDFHHRARIAMKPGITGMWQVSGRSDITDFEEVV
                     KLDAEYIRNWSMGLDFRILFRTVKTVLHRNGSM"
     gene            5759..6895
                     /locus_tag="SCJ86_002242"
     CDS             5759..6895
                     /locus_tag="SCJ86_002242"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297171.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HEG1169765.1"
                     /translation="MARKKLKICMLGHKRIPSREGGIEIVVEELATRMVALGHEVTCY
                     NRSGHHVSGKEFDQHKNKEYKGVKLKTIFTLNIKGIAAMSSSVFGGIRAAFGKYDVVH
                     FHAEGPCAMLWLPKLFGKRCVATIHGLDHQREKWNKLASTYIMLGEKCAVKFADEIIV
                     LSESVQNYFEDIYGRKTRFIPNGVKKIEIKSAGLITEKYGLTKDSYILFLGRLVPEKG
                     IRYLIEAFKDVQTDKKLIIAGGSSDTDEFANELKELAKGDERIIFTGFVQGQELEELY
                     SNAYIYTLPSDLEGMPLSLLEAMSYGNCCIVSNISECTEVVEDKAMIFKKSDVSDLKI
                     RLEEACNQSEMVKVLKNQATEFICSKYNWDKIVQETLNLYRGNI"
     gene            6895..8001
                     /locus_tag="SCJ86_002243"
     CDS             6895..8001
                     /locus_tag="SCJ86_002243"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343626.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 1 protein"
                     /protein_id="HEG1169766.1"
                     /translation="MIRILQCVNDMHRAGLETMLMNYYRNIDRTKIQFDFLTHRPYKS
                     DYDEEIISLGGKVYYAPRLYPQNYPKYFKWMEQFFKDHPEYEIVHSHIDAMSYLPLKA
                     AKKAGIPIRIAHSHNTSIDRDFKYLLKQYYRCKLTSVLTHEFSCGKVAGDFLFRNDKF
                     TLIPNAVDAKKFYYDESIRNDVRMKLGISSQTFVMGHIGRISYSKNHRFLIEIFKEFH
                     AIKNNSVLLIIGTGDMEEEIKNYAKKSGIDDDIKFLGNRNDIEKFYQAFDVLMLPSLF
                     EGVPLVGIEAQFADLPCFFSEKVPTEVAFSDKTNFISLNQSAKEWAREIANVDISHRD
                     SERSILIKADYNIQTAYKILESKYYELFELIQRG"
     gene            8007..9029
                     /locus_tag="SCJ86_002244"
     CDS             8007..9029
                     /locus_tag="SCJ86_002244"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343625.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="capsular polysaccharide synthesis protein"
                     /protein_id="HEG1169767.1"
                     /translation="MTQKHSSIKRIIHRISEELNATRRIAKITSWKEAFATLQAKIDI
                     QVMNRNGYNESKKQKEHLLKKHEVMLKYYEKTFSDFLKTYDYSQNQIINQSKSPYSNC
                     IWVCWWQGLDQSPEIVKACVKSIKRNAGKHTVIILTEDNYRQYVDIPKWVEEKKNKGI
                     ITRTNYSDLLRLSLLAKHGGLWLDATFYCTKPIPDEYFDIDIWSIRRPDYAHASVASG
                     YFAGYSLACNQESRWMFAIIRDFFLNYWKNNDMMVDYLMIDYMIVLAQRINHEIAQTF
                     DKIPSNNPLCDELIKNLNNIYDEKVWNLLKKDTLLFKLTWKQSFKKQKDDKVTFYGML
                     VENKLL"
     gene            9051..10106
                     /locus_tag="SCJ86_002245"
     CDS             9051..10106
                     /locus_tag="SCJ86_002245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HEG1169768.1"
                     /translation="MKHKVSVIVPIYNVEKYLRKCVDSLLSQTLKEIEIVLVDDGSPD
                     ASGEIADEYQKKYSNVKTIHRENGGLGPARNTGIENATGEYVAFLDSDDWVESDMYEK
                     LYLVASKENADIVVSGHCDFANDKAMVKKQHPLSGNVYDTPNSIKNVRKNLFGHSPKD
                     QEVEAFPMSVCMSLYNKNLLDKYRLRFEKILSEDTIFNLNAYDVANKIAFTEYTDYCY
                     RKEEQESITKSLSPNKQTEFKEFLITLKAIASKEDLECQIRAKRMAIDYCRLYVGLVD
                     DANLGIKEKRKYIKKFIEDEGLIQCWKGYPTNTLPFQQKFFHKMLECKCYTILLLLSR
                     IRKIIKNMTRKKLFIIL"
     gene            10724..11959
                     /locus_tag="SCJ86_002246"
     CDS             10724..11959
                     /locus_tag="SCJ86_002246"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321991.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEG1169769.1"
                     /translation="MSVKKMLFMVSTIIVLTSRFWGINTSLSMRYFIMAIWDLYFMLS
                     YFSYREKTYKKYVIKNNFWLTIMPFVIFSIYTLIIWGIGSNAEFENFIKLCSTLLYLI
                     LAYGYACTAFYLFKAEGIDFIFWSGVISYMSGSVFYLIVSYGLNGLISYIKSLITGET
                     NTANFAMEVHDLTFAMGVFFLYYVFFENKNTKKHKIKIIVSLILIFMGLKRIELLALS
                     GAILVYYVLLKWGKKIQIRALVCTICATVISLVFIYIIDTGILALLMSNLGITDDGAR
                     LSFYLYSSKFYELGLGYVGTGWTWFSKYFFNLYLSHFRLNGGRIAASLHSDILTIYIE
                     VGCIVFIIWMFWLFFVKPIRLTKKFGLKTGECVLLLTIYMFILYLTDNTLTYPDTQMI
                     FLLVPMIVSWVDNQQRISE"
     gene            12043..13131
                     /locus_tag="SCJ86_002247"
     CDS             12043..13131
                     /locus_tag="SCJ86_002247"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343622.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEG1169770.1"
                     /translation="MKKILVLANTYYQIIVAIQMKNTIFSDEYVEIIISDHSKNTKEI
                     AKKIEEKKVFDGCHYLESKSFDDNIGYFQKIKDCLQISCGKSNRYAYYLKNCKSEYFD
                     EFICFSMGEVWLEGIYACLYRNNKNVQVSILEEGILSYGVDIIPTARTKAIAVLNYLK
                     KRTPLYNKSYNFYCFFPNIYTGRENTIQIPLVTAGSRTALQLSDIFNPMIDIYKEKKY
                     IFFTSVYDFEGGEPVGEYELVCKIADLVGKDNLIVKTHPRDVRTIYKDNGFIEDKNSS
                     IPWEAIQLSGDFNDKIFMTINSGSVISGSTMSAKPVRTYYMYKLCNIEGNPSCKKNAY
                     DIERLLKQKTMKNILKNVKIAESLDDIL"
BASE COUNT         4854 a         1753 c         2625 g         4028 t
ORIGIN      
        1 tagtcaagaa aacagtttgt ggtatgatta tatagggaaa acttgtttac aaatatttct
       61 gtgaatatat tagtttaatt atgttttttt aaataaaaag ctgtcatttt tatgtaagtg
      121 aattatatct taatttatct atcatcaata cttaggagtg aatctatgga aaagccattc
      181 gtcaagtcat tgactgctgt ggcggtaggt gcaggtgcta ttgcgatctg tttgtttgga
      241 taccatatca ataatcagcg gcaacatcag caacggatca attatgcaga atctgccatc
      301 accaatcaaa aagacacctt gacttcttta tcaaaagaag tagacaagct gtatagtaca
      361 aaagaaaaaa tttttctaaa ccctgaaata acagaagaaa caatcacaag tttgtcaaat
      421 aagctatcct ctgtcaaact ttcagcagat gattacgaca tcaaagaatc agaattgcct
      481 aaagaagctg ttgcaatcca agatgagaag aaagcaatcg tgacacagtt agatagtgca
      541 gaatcaaaac tgaagatcca gacagctgtc aacgatttat ttactaaaaa tgtatcaaac
      601 tggcagcaag ctgtagacga tgtcatcatc aaagaaaagg tgacaagtgc agatgtcgct
      661 cgtgtacgtg aaaatatgag cttttttaaa gatagtcctt ggaaaacggt tgttatgcaa
      721 tatctaggat tcgctgatat tcagatcgcg caagtcacgc aactagatca gttattcgat
      781 acgatgttaa aggatggtca agtgactgcc accgctactt acgatcaata tttgacggtg
      841 ctcagtcaaa tagagcagat ccggaatgaa aaaataagcg cagcatacgc aacaaaagca
      901 caaactgttg ctgagcaaat gggctatagc aatataagct attgacagtt aaaacgttga
      961 ttcaagcaag gttagcaaac ggaggaaact atggcaagac gaagaagaaa atcaagaggg
     1021 aaaaaaatat ttctgattat tttagcagtt cttatcgtat tagtattagc aattgtagga
     1081 gtaggagcaa aattatatct ggatctctca cattctattg agaaaactta cgaatcagtg
     1141 gaacgaaccc aaggagaacg aacgaaacca gttgatctgg acaaacaaga atctttctct
     1201 gttctattga tggggattga tactggagat ttaggtcggg tcgatcaagg aagatcagat
     1261 acgatgatgg ttgccacagt cagtcctgaa gacaaacaga caacgatcgt cagcattcct
     1321 cgtgatacgt atgtcgacat cgtaggtcgt gacacaaaag acaagatcaa ccacgcttac
     1381 gcattcggcg gagctgccat ggcgatggat accgtacaaa aatatgtaga catcccaatc
     1441 gatcactatg tctctatcaa tatgaaaggg ctaaaggaac tcgttgatgc agttggtggt
     1501 atcgaagtca ataatgacct cactttctca caagatggct atgactttcc aatcggtaaa
     1561 attacattga acggggatca ggcattatct tattctcgta tgcgttatga agacccaaat
     1621 ggtgattacg gtagacaagg gcgccaaaga aagatcgtag aaggcgtagc gaaaaaagta
     1681 ttgagtctaa acggtgtcag caactatcaa tccgttttga atgctctaga gtcgaatatg
     1741 aaaacagaca tgaactttga tgaaatgaaa aaaattgctt tagattatcg ggaggcattt
     1801 ggtacgatta agcaagatca gctgcaagga gaaggcttca tgcaagataa tatctcctat
     1861 caaaaagtca gtgatcaaga acttgatcgt gtgaaaaaag aattaaaaga tcaaatgaat
     1921 ctagagaata aataggaaag tggagaaaaa atggaagaga caatcagctt gaaagaaatt
     1981 tttgaaacat tacgcaaaca cttggtaata atcatcatct caatgtttgc tggcttggcc
     2041 atctcaggaa tcgtaacatt tttcatcttg acaccaaaat acagttcaca agcacaattg
     2101 atcgtgacac tgccacaatc agacactgca aatgtcaatg atgtcaatac gaacctgcaa
     2161 atgatcaata cgtataaaga tatgatcgtc agtgatttag tgctcaatga agtcaaagat
     2221 cgtttagaaa cagaagacaa tgtgaaaatg actgctgaac aaatcaaaga tgccatctca
     2281 gttaatcagg cagaaaattc acaaatgttc tcgattcagg caataagtac gaatgcagtg
     2341 gctgctcaac agatcgctaa cacaacttca caagttttcc aagagaacgc aaaagatgtg
     2401 atgaatgttg ataaaatctc gatcatttca ggtgcagttg ccaatacgac acctgtttcg
     2461 ccaaataata aattgaattt aatgactggc ttaatgttag gaatgatggt aggtgtaggt
     2521 ctggcattct tattggaatt actcgatcgg acagtgaaag atgaaaaata tgtgacagat
     2581 acattaggat tcccaatctt aggaacagtt ccagaaatgt cagcgaaaga attgaatgct
     2641 ggtatccgta aaaaaggacc agctcagaca caaccaaaag ctacaaacga taaaacagcg
     2701 ccatcacgca gaagccgctc aagagtctag gaggaaagaa aatggcacga acacagaaac
     2761 aaaaagcagc ccaaactcat gcagtcagtt tggtcacact caccaatccc tcttcaccaa
     2821 tcgctgaaca atatcgtacg attcggacga atatccagtt tgcctccgca gctggtcagc
     2881 aaatcaaaac gattgttgtg acttcatcag gacctggaga agggaaatcg accactgcag
     2941 caaacattgc cgttgttttt gccaaatctg gtcaacgggt gctgctagtc gatgcagact
     3001 tacgtaagcc cgtcatctat aaaacgttca agttgaacaa cgcaagcgga ttaagtactg
     3061 ttcttagttc atcaggaagt gtagcagatg agatccaacg aacaccagtc gacaatttgt
     3121 cgattctgcc aagtggacct aaaccaccaa atccatcaga actactaagt tcgccaagaa
     3181 tggatcagat tttagcagaa gcaagacaac tatttgatgt agtgatcttc gacatgccgc
     3241 cagtcgttgc cgtaacagat gcacagatca tgtcttcaaa aacggatggc accttattgg
     3301 tcgtacggga aaatacgtca cgaaaagaat cattgactaa agcaaaagaa ctgttagata
     3361 tggtacaagc acgagtctta ggtgtcgtat acaacggagc agaacacagc aaagatgctg
     3421 gttattacta cggaaattaa ggaataagaa gagaggagac aagagaaatg atcgatctac
     3481 attgccatat cctaccagga atcgatgacg gggcagaaaa cttagaagca agtattgcaa
     3541 tggctgaaaa agcgattcag caaggcatta cgcatatttt atgcacacca caccacaata
     3601 atggaaaata cagcaatgaa aaatcacaag tcatttctct agtcgcttcg ttacaagccg
     3661 aattagagaa aagacagttg cctttgacgt tattggaagg acaagaagta cgtattaccg
     3721 gaacgctcat cgaagacatc caccgagatg aaattttatt tacagattta gatgatacat
     3781 atcttctgat cgaatttccg acactggaag tgccgttata tgcagaacgg cttttttttg
     3841 agttgttaga actaggaaag ataccagtga tcgtccatcc agaacgcaac gcgcattttc
     3901 gagaagaccc aaatcatttg cttccgttcc tagatatggg atgtttggcc caacttactg
     3961 cgccaagtta tgttggtgta tttggcaaag atatccagaa aacagccaaa acaatggtca
     4021 aacataatct cgtgcagatg gctgcttctg atgcacacgg ggtctctaag cgcacctttt
     4081 acttaaaaga gtgctatgag cagattgcca aggagtttgg taaggaaaaa gtagtacaga
     4141 tgaaacaagt cgcaaaggat ctgatcaacg gggatgagat caattatcca atctatgaag
     4201 aggtaaaaaa gaagaagttt ggtttatttt agttctttat tcgtagataa aaaggaaatg
     4261 aggagagagt gagagatgta tagaaaaaac tcaagtggtt ggtctaaaca tattgatttt
     4321 attttattag acttattttg tgtccagctt gctttttata tatcatatgt aatgcgacag
     4381 ggtgactgga atccgtatgt gattcctatt tatcgccaca tggcaatttt tatagaactt
     4441 gagaatctta cgattatatt tttatttgag gcatataaaa atgttttaaa aagaggatat
     4501 tataaagagg ctgtagcttc tataaagcaa tcttttatgc tgttacttgt gtgcagttta
     4561 taccttatta caatgcagga tggaaatgat tattccagag tttctttatt tataatgggg
     4621 gtggtgtatg gattattaac atatataatt cgtatacttt ggaaagggtt gttgcatcac
     4681 cgcatgaaaa acggtgaaga tgtatcgtta gttattgtaa catctcaaaa gattgcaaaa
     4741 aatgtagttc gtaatcttaa agagaagaat tatggcatgt ataaacttag tggtgtgatt
     4801 gtagtagaca gagatatgta tggaactacc atcggaggtg tcccagtagt tgcaaatgct
     4861 gataatgcag cggaatatct tttacagaat tgggtagatg aagtattcat caatgttgac
     4921 gaaagtgttc catatcccaa agaattaatc gccagatgtt cggaaatggg acttgctgta
     4981 catatgaatc ttacaaaagt gtctgatagt acacgtggaa aacaatttat tggccgagtt
     5041 ggtgattata ctgttattac atctagtatt aatgttatga cgatgaggca agcatttata
     5101 aagagaacga ttgacattct tgctggaatt attggatgta ttgcaacagg tgttattttt
     5161 atcttcgtag caccagttat ttacatcaaa tcaccagggc cgattttctt tgcacaagaa
     5221 cgtattggtc aaaatggtaa gacatttaag atgtataagt tccgtagtat gtatatggat
     5281 gcagaagaac gcaaagcaga gttgatgaaa gaaaataaga tgagtaataa tctcatgttt
     5341 aagatggatt ttgacccaag aatcattggc aataaaatac ttcctgatgg aacaaagaag
     5401 acaggaattg gacaatttat tcgatctaca tcattggatg agtttccaca attttataat
     5461 gtcctaatgg gaaaaatgag cctggtgggg tttagacctt gcttgaagtc agaatatgag
     5521 aagtatgatt ttcatcatcg tgcgagaatt gcgatgaaac ctgggattac tggaatgtgg
     5581 caggtgagtg gtagaagtga tatcacagac ttcgaagagg ttgtaaaact tgatgcagaa
     5641 tatatcagga attggagtat gggattagat tttagaattt tgtttaggac agtcaaaact
     5701 gtgcttcata gaaacggttc catgtaagat aaagatacta tagaaggagt aaaacgtgat
     5761 ggcaagaaaa aaattaaaaa tctgtatgct aggtcataaa agaattccat caagagaagg
     5821 tggaattgag atagtggtag aagaattggc aacgcgaatg gttgcacttg ggcatgaagt
     5881 gacttgctat aatcgaagtg gtcatcatgt tagcggaaag gaatttgacc agcataaaaa
     5941 taaagaatat aaaggtgtaa aattaaagac tatttttaca ttaaatataa aaggaattgc
     6001 agcaatgagt tcttcagtat ttggtggaat tagagcagca ttcggaaaat atgatgttgt
     6061 tcattttcat gcagaaggac catgtgctat gttgtggttg ccaaaacttt ttgggaaaag
     6121 atgtgttgca acaattcatg gtctagatca tcaaagagaa aaatggaaca agcttgctag
     6181 tacatacatc atgttagggg aaaagtgtgc tgtaaaattt gcagatgaaa taattgtact
     6241 tagtgaaagc gtccaaaatt attttgaaga tatatatgga agaaaaacaa ggtttatacc
     6301 aaatggcgtt aaaaaaattg aaataaaatc agcaggattg attacagaaa agtatggatt
     6361 aacaaaagat tcttatattt tgtttttggg gcgattggtt cccgaaaaag gaattcggta
     6421 tctaattgag gcatttaaag atgttcagac agataaaaaa cttataatag ccggaggaag
     6481 ctctgataca gatgaatttg caaatgaact aaaggaatta gcaaaaggtg atgagcgaat
     6541 tatatttact ggatttgtac aaggacagga attagaagaa ttatatagta atgcgtatat
     6601 ctatacactt ccaagtgatt tagaaggaat gccattaagt ttgttagaag caatgagtta
     6661 tgggaactgt tgtatagttt caaatatctc agagtgtacg gaagtagtag aagacaaggc
     6721 tatgattttt aagaaatcgg atgtaagtga tttaaagata agattagaag aggcatgcaa
     6781 tcaatcggaa atggttaaag ttttgaagaa tcaagctact gagtttattt gtagtaaata
     6841 taattgggat aaaatcgtac aagaaacatt aaatttgtat aggggaaata tataatgatt
     6901 cgaatattac agtgtgtgaa tgatatgcat cgtgcagggt tggagacgat gctaatgaac
     6961 tattatagga atatagatcg aactaaaatt cagtttgatt ttttgacaca tcgtccatat
     7021 aaaagtgatt atgatgagga aataataagt cttggaggaa aggtctatta tgcaccgaga
     7081 ctttatccac aaaattatcc taaatatttt aaatggatgg aacaattttt taaggatcat
     7141 ccagaatatg agattgttca ttctcatatt gatgcgatga gttatttacc tttaaaagct
     7201 gcaaaaaaag ctgggattcc gatacggata gcacatagcc ataatacatc gatagataga
     7261 gattttaaat atcttttaaa gcaatattat cgatgtaagt taacgtctgt tttgacacat
     7321 gaattttcat gtggaaaagt agcaggtgat tttttgtttc gaaatgataa attcactttg
     7381 attccaaatg cagtggatgc taaaaaattt tattatgatg aaagtattag aaatgatgta
     7441 cgaatgaaat taggaatttc aagtcagacg tttgtaatgg gacatattgg aagaatttca
     7501 tattctaaaa atcatcgttt tttgattgaa atttttaaag aatttcacgc tattaagaat
     7561 aattccgtat tattaattat tggaactggt gatatggaag aagaaataaa aaattatgca
     7621 aagaaaagtg gcatagatga tgatattaaa tttttaggaa atagaaatga tatagaaaaa
     7681 ttttatcaag catttgacgt attaatgttg ccatcattat ttgaaggggt tccgttagtg
     7741 ggtattgaag ctcaatttgc agatttaccg tgtttctttt cggagaaagt tcctacagaa
     7801 gttgctttca gtgataagac caatttcata agtttaaatc aatcggcaaa agaatgggcg
     7861 agagagattg caaatgtaga tatctctcat cgagattcag aacgttcgat tttaataaag
     7921 gcagattata atattcaaac agcatacaaa atcttagaat caaaatatta tgagttattt
     7981 gaattaatac aaagaggtta aagaatatga cacagaaaca ttcaagtata aaaagaatta
     8041 tacatcgcat atccgaagaa ttgaatgcaa caagacgaat tgcaaagata acatcttgga
     8101 aggaagcatt tgccacgtta caagctaaaa ttgatattca agtcatgaat cgtaatggat
     8161 ataatgaaag taaaaaacag aaggaacatt tattaaaaaa acatgaggta atgctgaagt
     8221 attatgaaaa gactttttca gattttttga aaacatatga ttatagtcaa aatcaaataa
     8281 ttaatcaatc aaaatcacca tattctaact gcatttgggt ttgttggtgg cagggattgg
     8341 atcaatcgcc ggaaattgta aaagcttgtg taaaatcaat aaaacgaaat gcagggaaac
     8401 atactgttat aattttaaca gaagataatt atagacaata tgttgatata ccaaaatggg
     8461 tggaagaaaa gaaaaacaaa ggaatcatta cgagaacaaa ttattctgat ttattaagat
     8521 tatctttgct agcaaaacat ggaggtttgt ggttagacgc cacattttat tgtacaaaac
     8581 caataccaga tgagtatttt gatatcgata tttggtctat taggaggcct gattatgctc
     8641 acgcatccgt agcatcagga tattttgcgg gttattcatt agcatgtaat caagagtcta
     8701 gatggatgtt tgctataatt agagactttt ttttaaatta ttggaaaaat aatgatatga
     8761 tggtggatta tttgatgata gattatatga ttgttttagc acagagaata aatcatgaaa
     8821 tagcacagac atttgataaa atacctagta acaatccatt atgtgacgaa ttgattaaaa
     8881 atttaaataa tatatatgat gaaaaagtat ggaacttatt aaagaaagat actttactgt
     8941 ttaagttgac atggaaacaa tcttttaaaa agcaaaaaga tgataaagtt actttttatg
     9001 ggatgttggt agaaaacaaa ttattataaa tattagaggt ataaaaagtt atgaaacaca
     9061 aggtaagtgt gatagtacca atttacaatg ttgaaaaata tttaagaaaa tgtgtggata
     9121 gtttattgag tcagacatta aaagagatag aaatcgtatt agttgatgat ggttcgccag
     9181 atgcttcggg ggaaatagca gatgagtatc aaaaaaaata ttctaatgta aaaacaatac
     9241 atagagaaaa tggggggtta ggaccggcaa gaaatacagg aattgagaat gcgacaggag
     9301 aatatgtggc ttttcttgat tcagatgatt gggtagaaag tgatatgtat gaaaaattat
     9361 acttagttgc gtccaaagaa aatgctgata tagtagttag tggacattgt gattttgcga
     9421 atgacaaagc tatggtgaaa aagcaacatc ctttgagtgg gaatgtatat gatacgccta
     9481 atagtattaa aaatgtacga aaaaatttat ttggacatag tccaaaagat caagaagtag
     9541 aggccttccc gatgtcagta tgtatgtctt tgtataataa aaatctgcta gataaatata
     9601 gactaagatt tgaaaaaatt ttatctgaag atacaatttt taacttgaat gcatatgatg
     9661 tcgcaaataa aattgctttt acagaatata cagattattg ttatagaaaa gaagaacaag
     9721 agtctattac aaaaagttta tctccaaata agcagacgga atttaaagaa tttttaataa
     9781 ctctaaaagc tatagcatcg aaagaagatt tagaatgtca aatcagagca aaacgtatgg
     9841 caattgatta ttgtaggctt tatgtgggct tagttgatga tgcaaatttg ggaattaaag
     9901 agaaaagaaa gtatataaaa aaatttattg aagatgaggg attaattcaa tgttggaagg
     9961 gatatcctac aaatacgcta ccatttcaac aaaaattttt tcataaaatg cttgaatgta
    10021 agtgttatac aatattgttg ttgttaagta gaattagaaa gataattaaa aatatgacca
    10081 gaaaaaaatt gtttataata ttataaacta aaagtatatc aaataataga aattaacaag
    10141 ttacaaaaaa atattataaa gaataatatt ttctaaaaag taaactatag taatgtggat
    10201 tatcaccttc caacagtata taaaatgagc gtaaaaacgg ctatttttgc cttaacaacg
    10261 atttttttgt atacaaatac gatatgctca ttaattattg gaatttgtag tgctgtttaa
    10321 attaatgaag gtgtaattat atgagtgatc attttgaaaa agtttgttga gaagatgtgc
    10381 ttctagtcat gtgatgggag aaaataattt aactgttaga gtgaatagtt aaattattct
    10441 ggttatatga aaaaagtagt ttctaaaata atcttgcagg gattaactta aagaaattga
    10501 gagaatagtt tctttgaaat ttctatatac aatgagaagc tgaaatttac ttttatttga
    10561 tgactggaaa tagaaattaa atatattgta ttgttacatt aaagaaaact tgtatggaaa
    10621 aatcagtaca ataccattat ttaatgttct tcacttaaag cgcaaaaaat atcagtttat
    10681 taaactcgta tatagaacaa atatatgtag acggggcgaa acgatgagtg taaaaaaaat
    10741 gctatttatg gtaagtacaa taattgtatt aacatcccga ttttggggaa ttaatactag
    10801 tttatctatg cgatatttta ttatggcaat atgggattta tattttatgt taagctattt
    10861 ttcttatcgt gagaaaacat ataaaaaata cgttatcaag aataactttt ggttgactat
    10921 aatgccgttt gtgatatttt ctatctatac tctaataata tggggaattg gttcaaatgc
    10981 agaatttgaa aattttataa agttatgttc cacgttgctt tatttaattc ttgcatatgg
    11041 ttacgcatgt acagcatttt acttatttaa ggcagaagga attgatttta ttttttggtc
    11101 aggtgttatt tcatatatga gtggttcagt tttttattta attgtatctt atggtttaaa
    11161 tggtttaatt tcttatataa aaagtcttat aacaggtgaa acaaatactg caaattttgc
    11221 tatggaggta catgatttaa cttttgcgat gggggttttt ttcctttatt acgtattttt
    11281 tgaaaataaa aatacaaaaa aacataagat aaaaattatc gtatctttaa ttttaatttt
    11341 catgggattg aagagaattg aactgttagc tttaagtggt gcaattctag tgtattatgt
    11401 tttattaaag tggggaaaaa agattcaaat ccgtgcatta gtgtgtacta tttgtgcaac
    11461 tgttatttcg ttagtgttta tttacattat agatacagga attttggcat tgcttatgtc
    11521 aaatttgggc attacagatg acggtgcgag gctttcattt tatctatatt catctaaatt
    11581 ttatgaactt ggtttaggat atgtaggaac aggatggaca tggttttcaa aatacttttt
    11641 taatctttat ttatcgcact ttaggttaaa tggtggaaga attgcagcaa gcctacacag
    11701 tgatatatta actatttata tagaagttgg atgtatagtt tttattatat ggatgttttg
    11761 gttatttttt gtgaaaccga taagactaac taaaaaattt ggattgaaaa caggggagtg
    11821 tgtattatta ttaactatat atatgtttat attatatttg acggataata cgctcactta
    11881 tccagacact caaatgattt ttctcttagt acctatgata gtttcgtggg ttgataatca
    11941 acaacgaata tcggaataga tagctaaagt atattttaat tatgaattta taattagttg
    12001 taaaagttaa tatttaacgg acaggaagta gggtgaaaaa atttgaaaaa aattttagtg
    12061 ttagcaaata catattatca aataatagta gcaatacaaa tgaaaaatac aattttttcc
    12121 gatgagtatg ttgaaattat tatttctgat cattctaaaa acactaaaga aattgcgaaa
    12181 aaaatagaag aaaaaaaagt gtttgatggc tgtcattatt tagaaagcaa atcttttgat
    12241 gataatattg ggtattttca aaagattaag gactgtcttc agatttcttg tggtaaatca
    12301 aatcgatatg cctattattt gaaaaactgt aaaagtgaat attttgatga atttatttgt
    12361 ttttcaatgg gagaggtttg gttggaagga atatatgctt gcttatatcg aaataataaa
    12421 aatgtacagg tatcaatttt agaagaaggt attttaagct atggagttga cattattcca
    12481 acagcaagaa caaaagctat cgcagtactc aattacttaa aaaaaaggac tccactatat
    12541 aacaaaagct ataattttta ttgttttttt cctaatatat atacaggaag agagaataca
    12601 atacaaattc cattagttac agctgggtca agaaccgcat tgcaactttc ggatattttt
    12661 aaccctatga tagatattta taaagaaaaa aaatatatat tttttacgtc tgtttatgat
    12721 tttgagggcg gagaacctgt cggagaatat gaattagtat gcaaaatagc agacttagta
    12781 ggaaaagata atctaattgt taagacgcat cctagagatg taagaacaat ttataaagat
    12841 aatggattta tcgaggataa aaattcatca ataccttggg aggcgattca attgtcaggc
    12901 gattttaacg ataaaatatt tatgacaatt aattcaggaa gtgttatttc aggtagtaca
    12961 atgtctgcaa aacctgtacg cacgtattat atgtataaat tgtgtaacat agaaggaaat
    13021 ccgtcttgca aaaagaatgc gtatgatatt gagcgattgc ttaagcaaaa aacgatgaaa
    13081 aacattttaa agaatgtgaa aatagcagaa agcttagatg atatactatg agcaaaacta
    13141 aatatttaat taaaaatatg ggaatattaa caataagtaa tttttcttca aaaattttag
    13201 tttttttgtt agtcccttta tatacaagtg tattatccac aacagaatat ggcacatatg
//
LOCUS       DARBET010000072        13060 bp    DNA     linear   BCT 07-NOV-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4027
            SAMEA114569841-rid20112183.denovo.074, whole genome shotgun
            sequence.
ACCESSION   DARBET010000072 DARBET010000000
VERSION     DARBET010000072.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA114569841
            Sequence Read Archive: ERR12203666
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 13060)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13060)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001972618.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001972618.1
            Genome Coverage       :: 132x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/07/2023 13:18:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,041
            CDSs (total)                      :: 2,975
            Genes (coding)                    :: 2,908
            CDSs (with protein)               :: 2,908
            Genes (RNA)                       :: 66
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 67
            CDSs (without protein)            :: 67
            Pseudo Genes (ambiguous residues) :: 0 of 67
            Pseudo Genes (frameshifted)       :: 26 of 67
            Pseudo Genes (incomplete)         :: 28 of 67
            Pseudo Genes (internal stop)      :: 20 of 67
            Pseudo Genes (multiple problems)  :: 6 of 67
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13060
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA114569841-rid20112183.denovo.074"
                     /isolate="VRE4027"
                     /db_xref="taxon:1352"
                     /country="Denmark"
     gene            66..539
                     /locus_tag="SCJ86_002248"
     CDS             66..539
                     /locus_tag="SCJ86_002248"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339235.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2975 domain-containing protein"
                     /protein_id="HEG1169771.1"
                     /translation="MKFKTLLLKFVVLGISAVFILFGVLFYTQIATSEKGFNFDWQTT
                     LFVSVIYFALLLGIVIAYFLLKLLKKIEDNQAFSTQSLNILKKIERTILFIGFAFFGL
                     LPKFMKVADLDDAPGLMLVGIAVVFIPFAVYTLTSVLEKLLEHAILLKTDHDLTV"
     gene            548..775
                     /locus_tag="SCJ86_002249"
     CDS             548..775
                     /locus_tag="SCJ86_002249"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008266675.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HEG1169772.1"
                     /translation="MAIIVNLDVVLAQKKMSVTELSEKVGITMANISILKNGKAKAIR
                     FSTLEKICQVLECQPGDLLTYKNDSNTIPEE"
     gene            1411..1782
                     /locus_tag="SCJ86_002250"
     CDS             1411..1782
                     /locus_tag="SCJ86_002250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339233.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEG1169773.1"
                     /translation="MPFIVYFFLSLLVIYIPLPTFFLLVNRLAEQHDTIAALTKLCLL
                     LFVCLDYFTSYYWLKQTRVKKRYYFFLVFFNLFEFLIHCFLLHQYHTIHLWIIWVFKV
                     VVLFCMFTFPISRKFREALFY"
     gene            complement(2038..2280)
                     /locus_tag="SCJ86_002251"
     CDS             complement(2038..2280)
                     /locus_tag="SCJ86_002251"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GlsB/YeaQ/YmgE family stress response membrane
                     protein"
                     /protein_id="HEG1169774.1"
                     /translation="MGFIWSLIVGGIIGAIAGAITNKGSSMGIIANVVAGLVGSALGQ
                     ALLGSWGPSIAGMAIVPSLIGAIILVLIVSFFARKL"
     gene            complement(2312..3214)
                     /locus_tag="SCJ86_002252"
     CDS             complement(2312..3214)
                     /locus_tag="SCJ86_002252"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296679.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Asp23/Gls24 family envelope stress response
                     protein"
                     /protein_id="HEG1169775.1"
                     /translation="MNNNNKNNNNKMNNQKPVSAHPDPTTPNQSNGVNDEKNKTAPVS
                     AHPDPVVPNVQQGVEEKKHDAEKHAPHHPGTPESKVPEQHDAIDPSKPVEEQKHPSDH
                     AKAQEKLSSDHTNGQGKLGSDHTNNQGKHDSEQEDSMRGELNYEDKVVQKIIGIAIEQ
                     VDGLLSANGGFFSNVAGKLVNTDNVTAGIETEVGKKQVAVDMDVIVEYGKDIEKIFEE
                     MQEVIGKEVKNMTHLEVIEVNANVVDIKTKEEFEKDQETVQDKVTDAAKKTGEFASNQ
                     TDKVKSAAGSGAQKVKENTEPRVQ"
     gene            complement(3227..3415)
                     /locus_tag="SCJ86_002253"
     CDS             complement(3227..3415)
                     /locus_tag="SCJ86_002253"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875246.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2273 domain-containing protein"
                     /protein_id="HEG1169776.1"
                     /translation="MKELFSTYKLPIIFGVLGLILAVLWITVGFFKTLLILIMAAIGV
                     AIGFYLKQTKILDHYFKS"
     gene            complement(3429..3992)
                     /gene="amaP"
                     /locus_tag="SCJ86_002254"
     CDS             complement(3429..3992)
                     /gene="amaP"
                     /locus_tag="SCJ86_002254"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alkaline shock response membrane anchor protein
                     AmaP"
                     /protein_id="HEG1169777.1"
                     /translation="MNKGVKTIGVIGTLLLLSVLLFAILLSATYPLPFQLEKFRFFSI
                     TNSYVQQYVFWLAVAFAILTIITLLVFLFYPKTIGNFLLKHGDGELTLDKKAIEGMVR
                     SHLHEDEFIHSPKVNVKATKNKIAVNVKGELKRTSALIGKTGTLMKEIENEVQHILGT
                     KEPVKVSVKYTGFEEPAKRSNDRKRVE"
     gene            complement(4030..4923)
                     /locus_tag="SCJ86_002255"
     CDS             complement(4030..4923)
                     /locus_tag="SCJ86_002255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629387.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HEG1169778.1"
                     /translation="MTEPKLIDPRNLYHTEKFPKQDQDTPALQQNMIPVPDCGEESYE
                     GNNQLENRRVLITGGDSGIGRAAAIAFAREGADIALQFFPGEEKDAEEVAKYVKDAGR
                     KIVLLPFDLRDEQAPKEIIDKAVSELGGLDTMVLNAGQQISCQSIAELPLEQVKDTFM
                     INIIAMFALVKEAVPHIPAGGAIVTTTSVQAFNPSEHLLDYAATKASIANFTIGLAKQ
                     LAPKGIRVNGVAPGPIWTPLQLDEGQPSDSIPEFGQQSLLERAGQPGELAPVYVFLAS
                     NKASYVTAQIYGVTGGEAINL"
     gene            complement(5001..5924)
                     /locus_tag="SCJ86_002256"
     CDS             complement(5001..5924)
                     /locus_tag="SCJ86_002256"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303839.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium transporter CorA family protein"
                     /protein_id="HEG1169779.1"
                     /translation="MDEEKNVIETDEKKYNWLVLDSAKPEEIEQAIEEFELPDDIFVG
                     TEYPEEVSRMERLYETKLEHPFSLVVINLDQSDNRIEKKLTPISFVLSDNLLITCIDR
                     KTEFVDRLLTKHGNHLNSFEKIITYTLLDIYTHYVKELREMKKRIDALDKAARKTTEN
                     EELYKQADLERDIVYIDHTLRDQRETMDQLWNSETFIKRLDDEQLLYDVHLRQRQTEK
                     MIEIYRDLLESIGDLFNGMMDNNLNHIMKYLDSAALIISVPALFAGIWGMNTGGLPGK
                     SSALGFILVVFGSLIAAVVLGYHLYRKDYTN"
     gene            complement(5973..6323)
                     /locus_tag="SCJ86_002257"
     CDS             complement(5973..6323)
                     /locus_tag="SCJ86_002257"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296672.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-sulfur cluster biosynthesis family protein"
                     /protein_id="HEG1169780.1"
                     /translation="MEITMSKNAVETLIEKVGENTKIALALINDSDPFLRDKGAFAKG
                     SFFQIIPFVSEFGEYATKIEHPLLDIYTSKLEQNYFGKRLNMDFNKQLDSFSLENEIA
                     VLDYNIKLKNCFFS"
     gene            complement(6356..7258)
                     /locus_tag="SCJ86_002258"
     CDS             complement(6356..7258)
                     /locus_tag="SCJ86_002258"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327003.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 8 protein"
                     /protein_id="HEG1169781.1"
                     /translation="MNKKEIAVVASCNTKFVPHLAALFVSVLDNCNPSKFVRFYVIDD
                     DIDFESKQLLRFSVKNARMNSDVEFLKINKEFFTNVVISDRIPETAYYRIAIPELFRG
                     TEVERILYMDCDMIALQDISKLWRLDFGDSIVAAVEDAGFHQRLEKMKISAKSMRYFN
                     SGLMLINVKKWLDENITQKVLDFIEHNPEKLRFHDQDALNAILHDRWLPLHPRWNAQG
                     YIMAKAKKHPTAAGEREYEETRNNPYIIHFSGHVKPWSKDFEGPTKKYYEKYAGMTAF
                     RCVAKFPKYPKYAKVQPKTDVVVK"
     gene            complement(7251..8108)
                     /locus_tag="SCJ86_002259"
     CDS             complement(7251..8108)
                     /locus_tag="SCJ86_002259"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329245.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 8 protein"
                     /protein_id="HEG1169782.1"
                     /translation="MEKRYGVVPVVTASDENYAPYLSVMIATALENCNKTRRIKFYVI
                     DDGLSEYSKEGLEETVNKYSSNASIQFLTVEKDIYEDFLVSDHITTTAYLRISLPNLL
                     AKEDYKKVLYLDSDVLVLDDIVKLYDEPLNGKTIGAIIDPGQVKALERLGIDSDDLYF
                     NSGVMVIDIDQWNKKEITEKTIHYLSENGDRIIYHDQDALNAVLYEDWEQLHPKWNMQ
                     TSLIFERHPAPNEKYERQYKEGNEKPSIVHFTGHDKPWNTLKDHPYTNLYLKKLAHST
                     LTKVGEINE"
     gene            8442..9251
                     /locus_tag="SCJ86_002260"
     CDS             8442..9251
                     /locus_tag="SCJ86_002260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629385.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cof-type HAD-IIB family hydrolase"
                     /protein_id="HEG1169783.1"
                     /translation="MKLAAIDLDGTLLDRNGKIPEENIQALRKFDENGGIVTVATGRN
                     SISAKDIFSELGIGGYLISSNGSLIAEMKDGKMAKVLRRSKIDSDTLRKAFFYAQEAK
                     ISIIASRETQDDQLTFDESNLVKDDPYYEHFNLQNHSFDQILNMLEDPSLSYLKLALT
                     DKSEEKLQKLQRDLQADGIQSVFSDPHFLEITPTGITKAHSLVFLADYLGIESSDIMA
                     FGDQENDIAMLNYSGLSIAMGNAQRQVKEIANQVTLTNEEAGVAYILKNFY"
     gene            9291..9788
                     /locus_tag="SCJ86_002261"
     CDS             9291..9788
                     /locus_tag="SCJ86_002261"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327002.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine hydrolase"
                     /protein_id="HEG1169784.1"
                     /translation="MLVIIDMQNHILDPNSEFYIPESDQLAERIAQRLAKARQNNEYI
                     LFTRDIPVELKDKEEEREDLQLIPLLSPQENERVIKKYYFTIPPETLAEIQETLFESK
                     EEQKEIEVVGIETNLCVLSNLIGLQSAFPEADFFVDPMLVSSRKHGESALELLKDFNV
                     SIVES"
     gene            complement(10173..10246)
                     /locus_tag="SCJ86_002262"
     tRNA            complement(10173..10246)
                     /locus_tag="SCJ86_002262"
                     /product="tRNA-Ile"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(10210..10212),aa:Ile,seq:cat)
     gene            10433..10756
                     /locus_tag="SCJ86_002263"
     CDS             10433..10756
                     /locus_tag="SCJ86_002263"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314960.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEG1169785.1"
                     /translation="MLGVIILPFIAILFDLVAAAAYFLQLNYQTSGILFIGMIFQGVI
                     TLLLLVMMLSYKGKRYARIQTKVFVKYVSIRYGIIILSFLVNAIVLFLYVLNYLDINP
                     LIFSR"
     gene            10920..11174
                     /locus_tag="SCJ86_002264"
     CDS             10920..11174
                     /locus_tag="SCJ86_002264"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335733.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEG1169786.1"
                     /translation="MITLVRVLFWVPAVALVASIVYLMNWNKERFYLAILTLPAIYFM
                     WKVFNYNYFEPDSVFIEELSGLVLSLLIVILYLIRLNKKH"
     gene            11244..11489
                     /locus_tag="SCJ86_002265"
     CDS             11244..11489
                     /locus_tag="SCJ86_002265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374660.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HEG1169787.1"
                     /translation="MSIEEAILLDTLRCRLNFLRTGRSLSQVAVSSALSIPLFKYRKY
                     EEGEEIPSIIELIYIADFYDISVDYLIGRSETASKLK"
     gene            11565..11930
                     /locus_tag="SCJ86_002266"
     CDS             11565..11930
                     /locus_tag="SCJ86_002266"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEG1169788.1"
                     /translation="MRLWHEKLISKLPRQQLLGQHRECCALRGNGWGKKHATVDYVFH
                     YSPYRLFQYHQLVMDEMEKRGYSPAPEWKNPMYRGKSCEAFFDLPTVERNVPIYPEHD
                     EVYLAECLANLEQKGIYLT"
     gene            complement(11991..12554)
                     /locus_tag="SCJ86_002267"
     CDS             complement(11991..12554)
                     /locus_tag="SCJ86_002267"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350857.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEG1169789.1"
                     /translation="MLYIKKGIIVISFFKEYLEIINFRLKTKKESVIILVYFQGGNLM
                     IKKLFLLSILTLAMAGCSDSGLATELITKSTEATTEELKCGNDHSDYRGLNRRCMGIL
                     GRSNCKHRCSNRRRCRRSNRKIKRKIKRSNLCCASYTDVEDAVLLNHVRRCSFMSREV
                     GDEPKLEYAFSFAREDEDNPKTYDILA"
BASE COUNT         3793 a         2433 c         2197 g         4637 t
ORIGIN      
        1 aaatagttgt tttacaataa ttattctgct atattttagc tatcaaagat aaaagaggga
       61 taaaaatgaa atttaaaaca ttgctattaa aatttgtcgt tttaggtata agtgccgtat
      121 ttattttgtt cggcgtactt ttttatactc agatcgctac aagcgaaaaa ggattcaatt
      181 ttgattggca gaccacatta tttgtttctg tcatttactt tgcccttctc ctagggatcg
      241 ttattgctta tttcttattg aaactattga aaaaaatcga agataaccaa gctttttcca
      301 ctcaaagctt aaacatacta aaaaaaatcg aacgaaccat tttgtttatt ggtttcgcct
      361 tttttggctt acttcctaaa tttatgaaag tagcggattt agacgatgct ccaggtttga
      421 tgttggtagg aatcgctgtc gtattcattc cttttgcagt atacacatta acaagcgtgc
      481 tggaaaaact gcttgaacat gcgattttac ttaagacaga tcatgatttg acagtgtgag
      541 gtgcgacatg gcaattatcg ttaatttaga tgtagtatta gctcaaaaaa aaatgagtgt
      601 gactgaattg agtgagaaag tgggcattac gatggcaaac atctctattc taaaaaatgg
      661 aaaagcaaaa gccatccgtt tttccactct cgaaaaaatc tgccaagttt tggagtgtca
      721 accaggtgat ttgttgacct ataaaaatga ctcgaatact ataccagaag aataaaataa
      781 agtagaacaa acagtaaata aaaaataatc tgaatgatag atgtgtgaga cttttagaag
      841 ttatttacgt ctattattca gattttttat ttcgattgaa taccgtttta tctataaata
      901 agatatgtac tgactcatac aaagggggcc ttttcttctc catgtactat cacttttcct
      961 gatacaactg gtttcctgtt acgttgtagt tggatctgct tttagtataa tactttattc
     1021 attccaccag actgatagca gtttgagcag tctaaataat catgtagtat tacaggccat
     1081 tctgtttcat accttgaagt gcttaaaact tcagctaatg gcccattgtg ttaactctta
     1141 ctccattctt ttttagtaag ctcccatata tactgtaaag ccttctatat attatgtcat
     1201 gttgcttttt aacatttact atgtagtttt gataaaatga tttgaataac agtgatggac
     1261 aattcttttt tatttttaac attattttga taataatgat tatttattta aaaatacatt
     1321 agataacatt tgttacgaca cgaaaaagct tcacaaacaa atatcgttat tttaaaatga
     1381 gggcgaactc acacaattag gaggaatagt atgccattta ttgtttactt ttttctttca
     1441 ttactcgtga tctatattcc attaccaaca tttttcctgc tcgtcaatcg tctagcagag
     1501 caacatgata cgattgctgc tcttacgaaa ctttgtttac ttctatttgt ttgtctagat
     1561 tactttacga gctattattg gctaaaacag acaagagtaa aaaagagata ttatttcttc
     1621 cttgtctttt tcaatctctt tgagtttctg atccattgct ttttacttca tcagtatcat
     1681 acgatccatt tgtggatcat ctgggttttc aaagtggtcg tattattttg tatgttcacc
     1741 tttcctatct caagaaaatt tagggaggca ttgttttatt aaagaagctt ctctgtatca
     1801 ataaagacac atttaaggaa aaacttttaa aaatctaatt gggaatttgc ccgcataaat
     1861 aagaggctgt gacaaaagtc ttgtcacagc ctcttattcc gtataaacag tgttcaaaag
     1921 cttgcatctt cgataatatg cccaaccaaa tgaaaaatat gagaagctat ttttgtttgg
     1981 ctgttcttat tacttgatga agaacagctt tttcacgacc tctttttaaa agtcttttta
     2041 cagttttcga gcaaagaatg atacgattag tactaaaatg atggcaccaa tcaatgatgg
     2101 cacgattgcc attcctgcaa tacttggtcc ccaagaacct aataatgctt gaccaagtgc
     2161 tgatccaact aaacctgcta cgacatttgc aataatcccc atcgaagaac ctttattcgt
     2221 gattgctcct gcaattgctc cgataattcc tccgacgata agtgaccaaa taaatcccat
     2281 gtttcgttcc tcctaatgtt ttacttcctt attactgaac ccgtggttct gtattttctt
     2341 ttactttttg tgcgccgctt ccagctgctg attttacttt atctgtttga ttcgaagcaa
     2401 attcaccggt ttttttcgct gcgtcagtta ctttgtcttg caccgtttct tgatcttttt
     2461 caaattcttc ttttgttttg atgtccacta cgttagcgtt gacttcgatc acttctaaat
     2521 gtgtcatatt ttttacttct ttgccgatta cttcttgcat ttcttcaaag attttttcga
     2581 tatctttgcc atattccaca atgacatcca tatcaactgc tacttgtttt ttaccgactt
     2641 ctgtttcgat cccagccgtt acattatctg tattcacaag ttttcctgcc acattagaga
     2701 agaaaccacc gtttgcagat aataaaccgt ctacttgttc aattgcgatt ccgatgattt
     2761 tttgcacaac tttatcttca taattcagct caccgcgcat tgaatcttcc tgttcagaat
     2821 catgctttcc ttggttattt gtatgatcag aaccaagttt tccttgtccg tttgtgtggt
     2881 cagaactaag tttttcttgt gctttcgcat ggtcagaagg atgtttttgc tcttccacag
     2941 gtttagaagg atcgattgca tcatgttgtt ctggaacttt agattctgga gttcctggat
     3001 gatgaggtgc atgcttttct gcatcatgtt tcttctcttc tactccttgt tgaacgttag
     3061 ggactactgg atctggatgt gcagatactg gtgctgtttt gttcttttca tcgttcacac
     3121 catttgattg gtttggtgtc gttgggtctg gatgtgcaga cactggtttt tggttgttca
     3181 ttttattgtt attatttttg ttattgttat tcatggatgt ttcctcctaa gatttaaaat
     3241 agtgatctaa gattttcgtt tgtttcagat aaaaaccgat agcgacgccg atcgctgcca
     3301 tgatcaagat caataaggtc ttgaaaaagc ccacggtgat ccataaaacg gctaatatca
     3361 atccaagtac cccaaagatg atagggagct tgtatgtcga aaatagttct ttcatttttg
     3421 gtccccccct attctactcg tttgcggtca ttgcttcgtt tagctggttc ttcaaagcct
     3481 gtgtatttca ccgatacttt cactggctct tttgttccca atatgtgttg tacttcattt
     3541 tcgatttctt tcatcaatgt tcctgtttta ccaatgagtg ctgaagttcg tttcaactcc
     3601 ccttttacat tgacggcgat cttgtttttc gttgctttga catttacttt tggtgaatgg
     3661 atgaattcat cttcatgcaa gtgagaacga accattcctt caattgcttt tttatccaac
     3721 gtcaactccc catcaccatg tttcagtaga aagtttccga ttgtttttgg ataaaacagg
     3781 aaaacaagta acgtgataat tgtcaaaata gcaaatgcaa cggcaagcca aaatacatat
     3841 tgttgcacgt atgaattagt gatggaaaag aatcgaaact tctctagctg aaaaggcaat
     3901 ggataggttg cacttaatag tatcgcaaac aaaagcacag atagcaaaag taatgttccg
     3961 atcactccga tcgtttttac acctttattc attttcgcac ctcctcatag gggtgaaacg
     4021 atcaacttct tataagttga tcgcttctcc accagttact ccgtaaattt gggctgtaac
     4081 ataacttgct ttattcgatg ctaagaaaac ataaactggt gctaattctc ctggctgacc
     4141 tgcacgttct agtaaacttt gctgaccaaa ttcagggatc gaatcagaag gttgtccttc
     4201 gtctagttgt aatggtgtcc agattggacc aggtgctaca ccatttacac ggattccttt
     4261 aggtgcaagc tgttttgcta aaccgatcgt aaagttagcg attgaagctt ttgttgctgc
     4321 gtaatctaat aaatgttcgc ttggattgaa tgcttgaaca gaagttgttg ttacgattgc
     4381 tcctcctgct gggatatgcg gaacagcttc tttcactaaa gcaaacatag cgatgatgtt
     4441 tatcataaat gtgtctttga cttgctcaag tggtaactca gcaatagact gacaagaaat
     4501 ttgctgtcct gcgtttagta ccatcgtatc tagcccgcca agttcactca cggctttatc
     4561 tatgatttct ttaggtgctt gttcgtcacg aagatcaaaa ggtagtaaga caatcttacg
     4621 tcctgcatct ttcacgtatt ttgctacttc ctctgcatct ttttcttctc ctgggaagaa
     4681 ttgaagagcg atatctgccc cttctctagc aaatgcaatc gctgctgcac gaccgatccc
     4741 tgaatctcca ccagtgatta gtacacggcg gttttctagt tgattattgc cttcatagga
     4801 ttcttctcca caatcaggaa caggaatcat attctgctgt aaagctggtg tatcctgatc
     4861 ttgtttagga aatttctccg tatggtacaa gtttcgcggg tcgattaatt ttggttctgt
     4921 cattgctgta ttcctcctag tttttaaatt gtattgccaa aacaaatttc tttgtttgat
     4981 acctgtttta aatagattct ttaatttgta taatctttcc ggtataaatg gtaacctaaa
     5041 actactgctg ctatcaaaga gccaaagact acgagtataa agcctagagc agaactttta
     5101 cccggtaatc ctcctgtatt cattccccaa atccctgcga acaacgccgg aacggaaatg
     5161 attaatgcag ctgaatctag atatttcatg atatgattta gattattgtc catcatcccg
     5221 ttgaatagat caccaatact ctctaataaa tctcgataga tctcgatcat tttttctgtt
     5281 tgtcgctgtc tcaaatggac atcatagagt agctgttcat cgtctaaacg tttgatgaaa
     5341 gtttcagaat tccataattg atccattgtt tcccgttgat ctcttaaggt atgatcgata
     5401 tatacgatgt ctctttcaag atctgcctgt ttatacagtt cttcattttc cgttgtttta
     5461 cgagctgctt tatccaaagc gtcaattcgc tttttcatct ctcttagttc tttcacgtaa
     5521 tgggtgtaaa tatctaataa tgtatacgtg atgatctttt caaacgagtt taaatgattt
     5581 ccatgctttg tcagcagccg atccacaaat tcggtttttc tatcgataca agtaatcaag
     5641 agattatcgg aaagtacaaa agatattggc gttaattttt tttcgatacg attatctgac
     5701 tgatctagat taatcactac caaagaaaat ggatgctcta gtttcgtctc ataaaggcgc
     5761 tccatccgag aaacttcttc cggatattcg gttcctacga aaatatcatc aggaagctcg
     5821 aattcttcga ttgcttgctc aatctcttcg ggctttgcac tatcaagtac gagccaatta
     5881 tatttttttt catctgtttc tattacattc ttttcttcat ccacaaagta gtatctcaat
     5941 gtatacctcc tttttggact ttctcgaaac tattagctga agaagcagtt ctttaatttg
     6001 atattataat cgagtactgc gatttcattt tctaatgaaa agctgtcaag ctgtttgttg
     6061 aagtccatgt tcaatcgttt accgaaataa ttttgctcta atttagatgt atagatgtcc
     6121 aataatggat gctcgatttt agtcgcatat tctccaaatt ccgaaacaaa aggaatgatt
     6181 tgaaaaaagc tgccttttgc aaaagcacct ttgtccctta gaaatggatc ggaatcattg
     6241 ataagcgcga gtgcgatttt tgtattttcc ccaacctttt caattaacgt ctctactgca
     6301 tttttagaca ttgtaatttc cattcctact ccccctcttc agttgttgtc ttctattatt
     6361 taacaactac atctgttttt ggttgaactt ttgcatattt tggatattta gggaatttag
     6421 ctacacaacg gaaagccgtc atccctgcat atttttcgta atatttcttc gttggccctt
     6481 caaaatcctt gctccacggt ttcacatgtc ctgaaaaatg gataatgtaa ggattgttgc
     6541 gagtttcttc gtattcacgt tcacctgctg ctgttggatg tttttttgct tttgccatga
     6601 tatagccttg tgcattccaa cgcggatgaa gtggtaacca acggtcatgt aagatagcat
     6661 ttaatgcatc ttggtcatgg aatcgtaatt tttctggatt atgttcgatg aaatctaata
     6721 ctttttgcgt aatattttca tctaaccatt ttttcacatt gatcagcatc aaacctgagt
     6781 tgaaataacg cattgattta gctgaaattt tcattttttc cagtcgttga tggaacccag
     6841 cgtcttcaac agctgctacg attgaatcac cgaagtcaag acgccataat ttagagatat
     6901 cttgtaaagc gatcatgtcg caatccatat ataagatacg ttcaacttct gtgccgcgga
     6961 acaattccgg aatcgcaata cgataataag ctgtttctgg aatacgatcg ctgattacca
     7021 catttgtaaa gaattcttta ttgattttca agaattccac atctgagttc attcgtgcgt
     7081 ttttcactga gaagcgtaaa agctgtttac tttcaaaatc gatatcatcg tcaatcacat
     7141 agaaccgaac aaatttagac gggttgcaat tatctaatac tgagacaaat aatgcagcta
     7201 aatgtggtac gaattttgtg ttacaacttg cgactactgc tatttctttt ttattcattg
     7261 atctcgccta ctttcgttag agtactgtgt gctaattttt ttaagtataa attggtatac
     7321 ggatgatctt tcaacgtatt ccaaggttta tcatgtcctg taaagtgaac gatcgaaggt
     7381 ttttcatttc cttctttata ctgacgttca tatttttcat ttggtgctgg atgacgttca
     7441 aaaatcagcg aagtctgcat gttccatttt ggatgaagtt gttcccaatc ttcataaagt
     7501 acagcattca aagcatcttg gtcgtgatag atgattcgat cgccattttc actgaggtaa
     7561 tggatagttt tttctgtaat ttctttttta ttccattgat caatgtcaat caccatcact
     7621 cctgaattga agtacaagtc atcggaatcg attcctaatc tttccaaagc tttcacttgg
     7681 cctggatcga taatagcgcc gattgttttt ccatttaacg gctcatcata tagttttacg
     7741 atatcgtcta gtactaacac atctgaatct aagtaaagga ctttcttata atcctcttta
     7801 gctaataagt ttggtaaaga aattcttaag taagcggtag ttgtgatatg gtcactgact
     7861 aggaagtctt cgtagatatc tttttccact gtcaggaatt ggatacttgc attacttgaa
     7921 tatttattta ccgtttcttc caatccttct ttactatatt ctgatagacc atcgtcaatc
     7981 acataaaatt ttatgcgtct tgttttattg caattttcta atgcagttgc gatcatcacg
     8041 ctcaagtaag gagcataatt ttcgtctgaa gcagttacca caggaactac gccgtatctc
     8101 ttctccatca aaatcactcc tttttctaat ctttttctaa cctgttctac atctagaata
     8161 ttcctcattt ggttttaaag caaaatatat gctttgtttt cattgaatat tttaaaattt
     8221 ttttatcaca aaacgcatcg gtaagcgcta actattcgtt tttcatggct ttttattcaa
     8281 aggtattgac caaaaactac tatatatagc cacctttttc ttaagaatgt tttttgaaaa
     8341 aattcataat gacaaagcaa gttagtttca tttttttaaa ttctcattta atcttaaggt
     8401 aacaggaaaa tagatatgta aaaagaaagt aggaagataa aatgaaacta gcagcgattg
     8461 atttagacgg taccttgctt gatcgaaatg gaaagatacc agaagaaaat atccaagcac
     8521 ttcggaaatt tgatgaaaat ggcggaatcg taaccgtcgc taccggaagg aacagcattt
     8581 ctgctaaaga tattttttcc gaattaggaa tcggtggcta tttgatttct tccaatggtt
     8641 cactgattgc tgaaatgaag gatggaaaaa tggcgaaagt tctcagacgt tcaaaaatag
     8701 atagcgatac actgagaaaa gccttcttct atgctcaaga agcgaaaata tccatcattg
     8761 ctagccgaga aacccaagat gatcagctga cattcgatga atcaaatcta gtcaaagatg
     8821 acccttatta tgaacatttc aatttacaga accattcttt tgatcaaatt ttaaatatgt
     8881 tggaagatcc ttctcttagc tatcttaaat tagcattaac agataagagt gaagaaaaac
     8941 tacagaagct acaaagagat ctgcaagcag atggtatcca atcagtattc tctgatccgc
     9001 atttcttaga aatcacgcct acaggtatca ctaaagctca ttcacttgtt tttctggcgg
     9061 attaccttgg aatcgaatcg agtgacatca tggcttttgg cgatcaagaa aacgacattg
     9121 ctatgttgaa ttacagcggg ttgagtattg cgatggggaa tgcacagcga caagtaaaag
     9181 aaattgcaaa ccaagtaaca ctgacgaatg aagaagctgg tgttgcttat attttaaaaa
     9241 acttctatta aaaagaataa aatgacgata tgaatgtagg aggaaagaaa atgttagtta
     9301 ttattgatat gcaaaatcat attcttgatc caaacagcga attttatatt ccagagtcag
     9361 atcaattggc agaacgtatt gctcaaagat tagctaaagc acgccaaaat aatgaatata
     9421 ttctcttcac acgggatata ccagttgaac tgaaggacaa agaagaagaa cgagaagatt
     9481 tgcaattgat ccctcttctt tctccacaag aaaatgaacg agtgattaaa aagtattact
     9541 ttacgattcc acctgaaact ttagctgaaa tccaggaaac tttatttgaa agcaaagaag
     9601 aacaaaaaga gatcgaagta gtcgggattg aaacaaactt gtgtgtactc tctaatctga
     9661 taggcctgca gagtgctttt cctgaagcag acttttttgt tgatccaatg ttagtctcca
     9721 gtagaaaaca cggagaatcc gctttagagc tgttgaaaga tttcaacgtc tctatcgtcg
     9781 aatcataaaa atatctatct tcatttacga tatttaacac acaataaaaa aagcacgcag
     9841 aactgtttta gtcctacgtg ctttttctat caaagtagat tggctttttt atgacacaga
     9901 gtgaaacggc aggctcacga cctctttctt aaatggtgtt caatcagctg accctcggta
     9961 ttagcttaac aaacggcaaa atatgaggaa ctatttttcc gtttgttaag ctaatactca
    10021 ggtcaaaacg ctgatttcac aacctcttcc ttaaacggta ttcaaaagct tgcatctgca
    10081 gtaataagaa cagccaagta aaaacataag aagctgtttt tacttggctg ttcttattac
    10141 ttgatgcagg gcagcttttt cacaacctct tatgtacctt gtaggactcg aacctacgac
    10201 cggacggtta tgagccgtct gctctaacca actgagctaa aggtacatca taaataaaat
    10261 ttatttttgt tacttttcaa ctatagcacg aatgacaaac tctcacaagt ttcaaaatat
    10321 aagtaaaata atattgttat tcttatttct tttacttgtg tcataactat tttgttactt
    10381 taatttttat ttgcttattt tagaaaaaat gttattcttt tatttattct caatgttagg
    10441 agtgattatt ttgccattta ttgcgatttt attcgattta gttgcggcag cagcttattt
    10501 tctccaatta aattatcaaa cctcaggaat tctgtttatt ggaatgattt ttcaaggtgt
    10561 gattaccctt ttgcttcttg tcatgatgct cagctataaa ggaaaaagat atgcccgtat
    10621 tcaaacaaaa gtatttgtta aatatgtttc cattcgctat gggatcatca ttctttcctt
    10681 tttagtgaat gcgatcgtcc tctttttata tgtattgaat tacttagata tcaatccatt
    10741 gattttttct cgatagataa gacacattca gcggaacagt tcctgcttat cagcagaaaa
    10801 ctgttctgct ttttcatttt tcattgatct attcttcttt tattttccta gcatttcatt
    10861 tgtgatgtat gcaataacat gttatctttt attatatgaa tcgttgagga gtccgttaca
    10921 tgattacgtt ggtgagagtt ttgttttggg tgcctgcggt cgcactggtc gcaagtattg
    10981 tttatcttat gaattggaac aaagaacggt tttatttagc tattttgact cttccggcta
    11041 tctactttat gtggaaagtt tttaattaca actactttga acccgatagt gtttttatag
    11101 aagaattatc aggactggtt ttgtctttat tgatcgtcat tctttatttg atacgtttaa
    11161 ataaaaaaca ttgagtacta ttgattgcat gtgtagcttt ctatagtcat tataactatt
    11221 ctactataat gaaaggtgat taaatgagta tcgaagaagc tattttactg gatactttac
    11281 gttgtcgtct gaatttctta agaactggcc gatctttatc tcaagtagct gtttctagcg
    11341 cgttaagtat tcctttattc aaatatcgta agtacgaaga aggagaagaa attccaagca
    11401 tcattgaatt aatctatatc gctgactttt acgatatttc cgttgattac ctaattggtc
    11461 gctcagaaac agcaagcaaa ctgaagtgat tttttccagc cttggtgctg gttttttcat
    11521 ttaacgtcta aggttaacaa tctatatata aaggagacat ttctatgcga ttatggcatg
    11581 aaaaattgat ttcaaagctt cctcgtcaac agcttttagg acaacatcgg gaatgctgtg
    11641 cattaagagg aaatggctgg ggaaagaaac atgctactgt ggattatgtt tttcactatt
    11701 ctccttatag attattccaa tatcatcagc ttgtcatgga tgaaatggaa aaacgtggtt
    11761 acagcccagc accagagtgg aaaaatccta tgtatcgtgg caaatcatgc gaagcatttt
    11821 ttgatttacc tactgtagag cgcaacgtgc cgatttatcc ggaacacgat gaagtttatc
    11881 tagcagaatg tttagcaaat ttggagcaaa aaggaattta tcttacctaa tataactaga
    11941 aaagccttct atctcctctt acttcattaa aaagctttaa ccatcttaga tcaagctaaa
    12001 atgtcgtaag ttttagggtt atcttcatcc tcacgtgcaa aactaaaagc gtactctagt
    12061 tttggttcat cgccaacttc cctagacata aatgaacatc tcctgacgtg gtttaaaagt
    12121 accgcatctt cgacgtctgt atacgatgca cagcacagat tgcttctttt gatctttctt
    12181 ttgatttttc tgttgcttct tctacacctt cttcgattac tacatcgatg cttacaattg
    12241 cttctgccta atattcccat gcatcgtcga tttaagcctc tgtagtcact gtgatcgttt
    12301 ccacatttta actcctctgt cgtggcttca gttgattttg tgatcagttc agttgctaag
    12361 ccagagtcag aacaacctgc cattgctagc gttaatattg atagtaaaaa tagttttttt
    12421 atcataaggt tccccccctg aaaatagact aaaataataa cgctttcttt ttttgttttc
    12481 agacgaaaat taataatttc taaatattct ttaaaaaagc ttataactat tatgcctttt
    12541 ttaatatata acacagttat ttttaaaaat aagaatgcta aatcactcta caataacggc
    12601 gaaagcacaa aaaataaaaa aattattacg ttattaaaaa aacagatgaa agatcaggtt
    12661 ttattagatc tcattctttc atcacttgaa gggatttaat gaactacttt ttcctctttg
    12721 tcgtttttat atattcgtat tccttcaaac agtaggatag cctctttcaa tgcttgatat
    12781 ttgaactgtc cgttacttaa aaatttagat gtgccgccct cttcctagaa catattcttt
    12841 tttacttttt cattaattga cagaccaatc gaacgaaact gtaaatttcg ataggataat
    12901 gattggtcaa actcattcat tttcaagaca tctcgattca tattcaagat aggaatcttt
    12961 ttatcaacat cactttattc gtattttcct ctaccataaa agcttcacgc tccacaaaat
    13021 tgttttatac actactattc agcccattca acgaccaatg
//
LOCUS       DARBET010000073        12957 bp    DNA     linear   BCT 07-NOV-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4027
            SAMEA114569841-rid20112183.denovo.075, whole genome shotgun
            sequence.
ACCESSION   DARBET010000073 DARBET010000000
VERSION     DARBET010000073.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA114569841
            Sequence Read Archive: ERR12203666
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 12957)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12957)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001972618.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001972618.1
            Genome Coverage       :: 132x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/07/2023 13:18:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,041
            CDSs (total)                      :: 2,975
            Genes (coding)                    :: 2,908
            CDSs (with protein)               :: 2,908
            Genes (RNA)                       :: 66
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 67
            CDSs (without protein)            :: 67
            Pseudo Genes (ambiguous residues) :: 0 of 67
            Pseudo Genes (frameshifted)       :: 26 of 67
            Pseudo Genes (incomplete)         :: 28 of 67
            Pseudo Genes (internal stop)      :: 20 of 67
            Pseudo Genes (multiple problems)  :: 6 of 67
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12957
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA114569841-rid20112183.denovo.075"
                     /isolate="VRE4027"
                     /db_xref="taxon:1352"
                     /country="Denmark"
     gene            complement(62..1171)
                     /gene="rpoD"
                     /locus_tag="SCJ86_002268"
     CDS             complement(62..1171)
                     /gene="rpoD"
                     /locus_tag="SCJ86_002268"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016176834.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor RpoD"
                     /protein_id="HEG1169790.1"
                     /translation="MANGTDKKTYEAAVAAFIKENKPKRQVVYDDLSNQLATPFTLNA
                     DEMDKLIQKIEDAGISVVDENGDPSIHSLKSAEKKAEQAKTEDLSAPTGVKINDPVRM
                     YLKEIGRVSLLTAEEEVALALKIEEGDQEAKQRLAEANLRLVVSIAKRYVGRGMQFLD
                     LIQEGNMGLMKAVEKFDYRKGFKFSTYATWWIRQAITRAIADQARTIRIPVHMVETIN
                     KLIRIQRQLLQDLGREPTPEEIGAEMDLPTEKVREILKIAQEPVSLETPIGEEDDSHL
                     GDFIEDQEATSPAEHAAYELLKEQLEDVLDTLTDREENVLRLRFGLDDGRTRTLEEVG
                     KVFGVTRERIRQIEAKALRKLRHPSRSKQLKDFLE"
     gene            complement(1195..3036)
                     /locus_tag="SCJ86_002269"
     CDS             complement(1195..3036)
                     /locus_tag="SCJ86_002269"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA primase"
                     /protein_id="HEG1169791.1"
                     /translation="MAQRIPQEVIETVRSQTNIVEVIGQYVQLKKSGKNYLGLCPFHE
                     ERTPSFSVAEDKQIFHCFGCGKGGNVFTFLQELEGLSFPEAVVKVADFEQIPLEDQWR
                     QRTIAVQNPESATGKLIAAHEKAAEIYHHMLLHTKAGQPALEYLQSRGLSLELIEEFN
                     IGFAPNERSFLQQVFKNESISNELLERSGLFVTHDDGRMSDRFYQRVMFPLRNSQGQT
                     IGFSGRWLAPEKGQEKDQPKYLNSPETELFNKRQVLFNLDKARSDIRKNGEILLFEGF
                     MDVIAAWQAGIKTGLASMGTSLTAQQIQQMEKLTKELVFCYDGDNAGFEATNRGIELL
                     RQNSRLALSVVVVPEKLDPDEYLRKYGEASFQELVAHGRETVFTFKSRYLKQGKNLDN
                     EKDKIEYLEELLVELSRVVSPIEQDMYLSQLSTEFQIGRETIQKQLRDLRRTNQQNRQ
                     EPQESQHTQVTRQETARKKRPLTQVEKAEQILLYRAFKEVSVRNILKEREFQFIHDAY
                     AEVYFLFDAYVSQHYEFNLAEFLDFLQDENLRRLVVEIEYLPVAEESSPREIQDLLDV
                     ISKSGLADEITLKKQMQQEARRTGNKQLELELTIEIINLTKQLKQAK"
     gene            complement(3218..5533)
                     /locus_tag="SCJ86_002270"
     CDS             complement(3218..5533)
                     /locus_tag="SCJ86_002270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342697.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation-translocating P-type ATPase"
                     /protein_id="HEG1169792.1"
                     /translation="MNGLTQQEVVQKMASGQQNDYQEDASKSTKQIFSDNILTLFNFL
                     NFGIGICLLFVGAYSNMAYLAIIVVNIVIGIYQEIHARNLVRKLSIVSQSEVLVIRDG
                     KEQSISTKELVLGDLVKIGAGEQIPADLQVISGRAEANEALLTGESDLIVKETGAELL
                     SGSFLTSGSVMAEVIRVGADNYAVRLTSEAKTHKAIRSELVDSIRKVSKFTSFVIIPL
                     GIILFLEALFIRDASIKISVIASAAALLGMLPKGLVLLISIALTTGVTKLAKKRILVQ
                     DMYSIETLAHVDTLCLDKTGTITEGKMSIQRVEALHPLYEKAIPEIMGTYLAESSDNN
                     ITMRAMRDYFELSNRYDVTNKVAFSSDRKWGAMEFPELGVVYVGAPERLVEEDRLPDS
                     LKTAQANGYRVLMLGIAPNQQLNEEKPAFVEPVALFEIDDPIRKNAQETLAYLHEEGV
                     DLKVISGDNPFTVSNIARRAGLPNYDAYVDLSQITEEMEVREAAHRYTVFGRVSPQQK
                     KLLVNELKESGRTVAMTGDGVNDVLALREADCSIAMAEGDGATRQIANLVLLDSDFTT
                     LPEVLFEGRRVVNNVTKVSGIFFIKTIYSFILSIICAVTAIGFPFIPIQITLIDLAIE
                     GYPSFFLSFEQDKRKITYRYLPTALINALPNALLVVVNIIAVYLLGKTFAWGNAETTT
                     LMYYLLIGISCMAVVKACYPFNPLRIFLAVTTIVGIYVAAMLFHGILEVNLGLGESVG
                     YFFVFMLVNIVLRVLYWKADIQNQLLKRVAN"
     gene            complement(5718..6578)
                     /locus_tag="SCJ86_002271"
     CDS             complement(5718..6578)
                     /locus_tag="SCJ86_002271"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629620.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF368 domain-containing protein"
                     /protein_id="HEG1169793.1"
                     /translation="MEKRQKMENQEVGYTTKDWMLRFVKGMFIGSGFILPGVSGGALA
                     AVFGMYERLISFLAHLTKNFKENVLFFLPVGLGGVTGIFLLSFLVSFLLGAYETTILW
                     FFVGCIAGTVPALWKEAGKKGRDTSDVIIMVVTFVLAYLFLLYGAQLFDGQVDQNFGT
                     WLIAGGLIGLGMIVPGLSPSNFLVYMGLYKAMADGFKEVRLEVIVPIGIGGILCVLGL
                     SKIMDYIFSKAYSKLFHFILGVVFASTIMIIPTDYAGLGVAGVLACPVLFIAGTALGW
                     WMSRLEEQYK"
     gene            complement(6637..7158)
                     /locus_tag="SCJ86_002272"
     CDS             complement(6637..7158)
                     /locus_tag="SCJ86_002272"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303352.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysozyme family protein"
                     /protein_id="HEG1169794.1"
                     /translation="MESYQIEITTAAKNYHMENYENLIKAIILTESKGKGTDLMQSSE
                     SLHGEAEIIDNPADSIDQGVKYLAEMIVEAKKQGCDLWTAVQAYNFGKDYIAYVKNHG
                     GENTVELAEAYSKDVLSPLLGNEEGKQYRYWRIQSLLYNGGYLYHNGGNMFYPDIVKM
                     NEKIIETYEKVFK"
     gene            7436..8197
                     /locus_tag="SCJ86_002273"
     CDS             7436..8197
                     /locus_tag="SCJ86_002273"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289461.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HEG1169795.1"
                     /translation="MTDIIVILYTVGDYVKKEKKYAKMPDGSEIYYEKSGQGFPLFLL
                     HGNDGSGRFFSEQVPVLERYYTVYLVDSRGHGRSTNEASMLNFQLMAEDLNTIMLLEK
                     IDQADFLGFSDGANLALVFASSFPKKVHRLILNSGNTLVKGVRFSARVISNIHYAWVW
                     LLSLFRPSLRKNLLVIKLLLHDIGLTENDLKKINSPTLIIVGKKDVIKLKHSLYIAKT
                     IPKASFVLVKEQGHELARKDPERFNREVLQFLSET"
     gene            8223..10814
                     /gene="mprF"
                     /locus_tag="SCJ86_002274"
     CDS             8223..10814
                     /gene="mprF"
                     /locus_tag="SCJ86_002274"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290133.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional lysylphosphatidylglycerol
                     flippase/synthetase MprF"
                     /protein_id="HEG1169796.1"
                     /translation="MLKNTIQWLKNHLGLFKTIFLISVIVIIVGELMSIGKTLSIQQL
                     GETFATIPVWKSGLMLLIGLISVLPMVGYDIILNRMLEQKQNPRYLFETSWLINTINN
                     IAGFGGFVSVGLRSELYGQKKESRNVIQALSKVFLFLMAGLSIYSLLSFLLVVFTPVA
                     PFLKQYWIWLIGGGLYFPVVFLFTTFKKKGFLGGTSLKTQGQLLLVSLLEWTGVLFSF
                     LSVGYLMEIRIDLWQTIPLFIAASVIGIVSMIPGEIGSFDVMMIIGLSAIGVPREIVV
                     VWILLYRLFYYIIPFLIGIVFFFKNIGSTFDQRYSGIPKQLATEIAHKIVVVLLYFSG
                     IMLVLSATIPQAFTEFRWLHSLNPLKFHFIIQFPSILLGFLLIIMGRGIAARVKRAYL
                     PTIFLIVLALLYVLLSDFSFTPVVFLSILLLIILASKNELFREQLIYSWEWRTIDGII
                     IGALSLLYIVIGVYNLPDFPHRRHHFISFFLFPSEKIWFSGLLAIIAVSFMIVLFVHF
                     LQGEKKQIGEAFNEEKALKILTTYGGNSDSQLIFLKDKRMFAYEKDGEPTVLLQFACF
                     NNKCIVMGDPSGKKEDFPEAIEAFIEETDRLCYLPVFYETSEEIVMILHEFGYDFIKM
                     GEEAYVDLNSFTTSGKKMKGTRAVLNRIEREGFTFDVLQPPFSTEQMSTFKNISDNWL
                     GSRKEKGFSLGFFSEDYLQRAPIAVVKNTQEEIVAFATIMPTYTNNKVGTIDLMRYDS
                     KKAPSGSMDFLFLHLFAYMKEEHIQWFNLGMAPLSNVGTSRKSFLQERIAYLVYEFGS
                     HFYSFHGLREYKSKYATNWVSRYTLYSRDSWIAYVMIALLIIDNAPVEQTQGRISGLK
                     KWIRRKY"
     gene            complement(11002..12189)
                     /gene="tuf"
                     /locus_tag="SCJ86_002275"
     CDS             complement(11002..12189)
                     /gene="tuf"
                     /locus_tag="SCJ86_002275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005878630.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor Tu"
                     /protein_id="HEG1169797.1"
                     /translation="MAKEHYDRSKPHVNIGTIGHVDHGKTTLTAAITTVLGKKGLANP
                     QDYASIDAAPEERERGITINTAHVEYETEKRHYAHIDAPGHADYVKNMITGAAQMDGA
                     ILVVSATDGPMPQTREHILLSRQVGVKYLIVFLNKVDLVDDEELIDLVEMEVRELLSE
                     YGFPGDDTPVIKGSALKALQGDPDAEAAIMELMDTVDEYIPTPERDTDKPLLLPVEDV
                     FSITGRGTVASGRIDRGAVRVGDEVEIVGIKPETQKAVVTGVEMFRKTLDYGEAGDNV
                     GVLLRGIQRDDIERGQVLAKPGSITPHTKFKAEVYVLTKEEGGRHTPFFNNYRPQFYF
                     RTTDVTGTITLPEDTEMVMPGDNVTIDVDLIHPIAVENGTTFSIREGGRTVGSGIVTE
                     IEA"
     gene            12557..12913
                     /locus_tag="SCJ86_002276"
     CDS             12557..12913
                     /locus_tag="SCJ86_002276"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289464.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YxeA family protein"
                     /protein_id="HEG1169798.1"
                     /translation="MKKLIGVLVVLGIFLGGSFAAYHYFYGGEAYYTKITTNGEMSAT
                     QADNGEKFTTYTYKQAAYNKDGEKKQVTLREEREHPLKMNAYLKLKVNPRKGVLSWEE
                     IKASEVPKNAAEKIDN"
BASE COUNT         3717 a         2710 c         2307 g         4223 t
ORIGIN      
        1 tttttctttt aaaataaacc aaacagtttt actgatttct cttctggtca atagtccacg
       61 cttattctaa gaaatctttt aattgttttg aacgagaagg atggcgcaat tttcttaatg
      121 ctttagcctc gatctgacga atacgttccc gagtaacacc aaatactttc cctacttctt
      181 ccaatgttct cgtccggcca tcatctaaac caaaacgtaa gcgaaggaca ttctcttccc
      241 gatcagtcaa tgtatctagt acatcttcca attgttcttt tagtaactca taagctgcgt
      301 gttctgctgg acttgttgct tcctggtctt ctatgaaatc acctaagtga gagtcatctt
      361 cttcaccgat tggcgtttca agggaaacag gttcttgcgc gattttcaag atttctcgga
      421 ctttttctgt tggaagatcc atttccgcac cgatttcttc tggcgtaggt tctcttccaa
      481 ggtcttgcag caattgacgt tggatacgta tcaatttatt aattgtttca accatatgaa
      541 ctggaatacg gatcgtacgt gcttggtcag cgattgctcg agtgatggct tgacgaatcc
      601 accatgtagc ataggttgaa aacttaaatc ctttgcggta gtcaaatttt tcaactgcct
      661 tcatcagtcc catgtttcct tcttggatca agtcaaggaa ctgcatgcct cgtcctacat
      721 aacgtttagc gatacttact actaaacgaa ggtttgcttc tgccagacgc tgtttcgctt
      781 cttggtcgcc ttcttcgatt ttcaatgcta acgctacttc ttcttccgca gttaaaaggg
      841 agacacggcc aatttctttc agatacatac gaaccgggtc attgattttt acaccggttg
      901 gcgcagaaag atcttctgtt tttgcttgtt cagctttttt ctcagcactc ttcaagctgt
      961 gaattgatgg atcaccattt tcatccacca cactgatacc tgcgtcttca atcttttgga
     1021 tcaatttgtc catttcatct gcattcaaag tgaatggtgt agcaagctga ttggataaat
     1081 catcatatac aacttgtcgt ttgggtttgt tttctttgat gaaagctgct actgctgctt
     1141 catacgtttt tttatctgtt ccatttgcca taaaagaagg ccccccttga gtagctattt
     1201 ggcttgtttt aactgtttcg ttaaattgat gatttcaatc gtcaattcaa gctcaagctg
     1261 tttgtttcca gttcgtctgg cttcttgctg catttgtttt tttaatgtta tttcatcagc
     1321 taagccagat ttgctgatca catctagcaa gtcttggatt tctcgtggcg aactttcttc
     1381 tgccaccggt aaatattcga tttccaccac caaccgcctt aaattttcat cttgcaaaaa
     1441 gtctaaaaac tctgcaaggt tgaattcata atgttgtgag acatatgcat cgaataaaaa
     1501 ataaacttct gcatatgcat catgaataaa ctgaaattct cgttctttta aaatattacg
     1561 gacagaaact tctttaaatg cacgatataa tagaatctgc tctgcctttt ctacttgcgt
     1621 caaaggtctc tttttccgtg cagtttcttg tctcgtaacc tgagtgtgtt gactttcctg
     1681 aggttcttga cgattttgct gatttgtcct tcgaagatcg cgcagttgtt tttgtatcgt
     1741 ctctctgccg atctgaaact ctgttgacag ttgagataga tacatatctt gttcgattgg
     1801 tgaaacaact cggctaagtt caactagtaa ttcttcgagg tattcgatct tgtctttttc
     1861 attatctaaa tttttccctt gttttaaata acgtgattta aaagtaaaga ctgtttcgcg
     1921 cccatgagcc actaattctt ggaaagatgc ttctccatac ttacggagat attcatcagg
     1981 atcaagcttt tctggaacga ctacgacaga aagtgctaat cgactgtttt gccgaagcaa
     2041 ttcaatccct cgattggttg cttcaaaacc tgcgttgtct ccgtcatagc aaaagaccaa
     2101 ttctttcgtc agcttttcca tttgttggat ctgttgagct gttagacttg tccccataga
     2161 agctaatcca gttttgatac cagcctgcca tgcagcaatc acatccataa acccttcgaa
     2221 caacaaaatt tctccgtttt tccggatatc gcttcttgct ttatcaagat taaataaaac
     2281 ttgtctttta ttgaacagtt ctgtttctgg actattcaag tatttgggct gatctttttc
     2341 ctgccctttt tcaggtgcaa gccagcgtcc tgaaaaaccg atcgtctggc cttgagaatt
     2401 acgaagtgga aacatcactc tttgataaaa acgatcagac atacggccat catcatgcgt
     2461 gacaaacaat ccagagcgtt ctaacaattc atttgaaatt gattcattct tgaatacttg
     2521 ctgaagaaaa gaacgctcat taggtgcaaa acctatgttg aattcttcaa ttaattctaa
     2581 agacaaccca cgactttgca aatattctaa agctggctgt ccagcttttg tatgcaatag
     2641 catgtgatga tagatttccg ctgctttttc atgagcagca atcagtttcc ccgtagcaga
     2701 ttcaggattt tggacagcta tagtacgctg acgccattga tcttctaacg gtatctgttc
     2761 gaagtctgcc acttttacca ctgcttctgg aaaacttaga ccttctagtt cttgaagaaa
     2821 agtaaacacg tttccgcctt taccacaacc aaagcaatga aaaatctgct tgtcttctgc
     2881 aacagaaaaa gacggtgtac gttcttcatg aaaaggacac agccccaagt aatttttacc
     2941 agactttttc aactggacat attgtccaat cacctcaacg atattcgttt gactgcgtac
     3001 tgtttcgata acctcttgag gaatccgttg agccattttt tcaccccctg ctctattgat
     3061 atcttttggg tttaacagaa gatatttcag gagaataacc ggtaaaaaat cgcactaact
     3121 gaaatcagtg cgattttaaa ggacacaatt atacatgttt gattgtatca tatttcattg
     3181 ctttttcaag tataacacat ttctatctca ttaagcatca atttgctact cttttcagta
     3241 actgattttg aatatcagcc ttccagtaca atactcgaag aacaatattt acgagcatga
     3301 atacaaagaa gtatcctacg ctctctccca accctaagtt tacttccaaa attccatgga
     3361 acagcattgc agctacatat atccctacga tcgtcgttac tgctaggaag atgcgcaatg
     3421 ggttgaaggg ataacaagct tttacaactg ccatgcaact gatcccaatc agcaagtaat
     3481 acatcaaagt agtagtttca gcatttcccc atgcgaatgt ttttccaagt agatacaccg
     3541 caatgatatt cactactaca agcaaggcat ttggtaaagc attaataagt gctgtaggca
     3601 aataacggta agtgattttt cttttatctt gttcaaaaga taaaaagaag gaaggatacc
     3661 cttcaatcgc taaatcaatc agcgtgattt ggatagggat aaacggaaat ccaattgctg
     3721 taacggcaca tataatcgat aaaataaatg aataaatcgt tttgataaaa aagatgcctg
     3781 agactttcgt cacattgttg accactcggc gtccttcaaa caggacttct ggcaatgtag
     3841 taaaatcaga gtccaacaat accagattcg caatctgacg tgttgctcca tcaccttctg
     3901 ccatagcaat gctgcaatct gcttctctta acgccaaaac atcattcaca ccgtcgcctg
     3961 tcattgcaac cgttctgccg ctttctttca attcatttac cagcaatttc ttttgctgcg
     4021 gactcactcg tccaaaaacc gtatagcgat gagcagcttc tcgcacttcc atttcttcag
     4081 taatttgaga aagatcgaca tacgcatcat aattcggtaa tcctgcacgt ctagcgatat
     4141 tggagacagt aaaaggattg tctccagaaa tcactttcag atccacacct tcctcatgaa
     4201 gataagctag tgtttcttga gcgttttttc ggattggatc atcgatttca aacagagcaa
     4261 ccggctcaac aaatgctggt ttttcttcgt tcaattgctg atttggagcg atccccaaca
     4321 taagtacgcg atagccattc gcttgggctg tttttagact gtccggaagc cgatcttctt
     4381 ccaccaaacg ttcaggcgct cctacgtaca cgactcccag ttctggaaat tccatcgcgc
     4441 cccatttgcg atcagaagaa aatgcgactt tgtttgtcac atcataccga ttagaaagct
     4501 caaaataatc tctcatagcc cgcatagtga tgttgttatc actgctttct gccagatacg
     4561 tccccataat ttctgggatg gctttctcgt acagcggatg tagtgcttct acccgctgga
     4621 tactcatttt cccttcagta atggtccctg ttttatccaa gcataatgta tcgacatggg
     4681 ccagcgtttc aattgaatac atgtcttgaa caaggattcg tttttttgcc agtttggtta
     4741 cccctgtcgt taatgcaata cttattagca ataccaaacc ctttggaagc atgcctaaca
     4801 aagctgctgc agatgcaata acagaaattt tgatagaagc atcgcggatg aacagtgctt
     4861 ccaaaaatag tatgatcccc aaaggaataa tcacgaaact agtgaatttg gatactttgc
     4921 gaatcgaatc tacaagttca gaacgaatag ctttgtgagt ctttgcctcg ctagtcaagc
     4981 ggacagcata attatctgca cctactcgga taacttcagc catgacagaa ccgcttgtca
     5041 aaaagcttcc agaaagcaat tcagctccag tttctttgac aatcaaatca gattcgcctg
     5101 tcaaaagagc ttcatttgct tctgcccgtc cagatataac ctgtaaatcg gctggtatct
     5161 gttctcctgc tccgattttt accaaatctc ctaataccag ctctttagta gaaatcgatt
     5221 gttctttacc gtcccggatc actagtactt ctgattggga aacaatcgat aatttccgta
     5281 caagatttct tgcatgtatt tcttgataaa ttccaataac gatattgacg acaataattg
     5341 ctaaatacgc catgttggaa tacgcaccta caaataacag acaaatccca ataccaaagt
     5401 tcagaaaatt gaataacgtc aatatattat cgctgaatat ttgttttgta ctttttgatg
     5461 catcttcctg gtagtcattt tgctggccag aagccatttt ctgaactacc tcttgctgtg
     5521 taagtccatt cattttcttt ttctccattt tccgccaact ttcaaaaata atcacgtgtt
     5581 taagtctagc gtgttccaag attaaaaaca acttgttttt ataaaattta tagaaaaaca
     5641 cacgaatgat tttacaaaaa agatgccaac ctttttgttt gaatcacccg tgtgctgttt
     5701 ttattatcaa tctatcttta cttatattgt tcctccaaac ggctcatcca ccaacctagt
     5761 gctgtaccgg ctataaaaag aacaggacaa gctagtaccc cagcaactcc caatcccgcg
     5821 taatcggtag gaatgatcat aatcgttgaa gcaaaaacta cacctaaaat gaaatggaat
     5881 agcttgctat atgcctttga gaaaatgtaa tccatgattt ttgacaatcc aagaacacaa
     5941 agaatcccac caattccgat cggcacaatc acttcaagtc gtacttcttt aaatccgtca
     6001 gccatcgctt tatacaatcc catatacacc aaaaaattag aagggctcaa tcctgggacg
     6061 atcataccca aaccaatcaa tccgccggca atcagccatg ttccaaaatt ctgatcgact
     6121 tgcccgtcaa ataattgagc gccgtataat aaaaacaaat aagccagaac aaaagtaaca
     6181 accatgatga tcacatcaga agtatctctt ccttttttcc ctgcttcttt ccataacgca
     6241 ggaacagttc cagcgataca tccgacaaaa aaccataata tcgttgtttc atacgcacct
     6301 aataaaaaac tgactaaaaa agaaagtaga aagatgcctg tcactccgcc tagaccaaca
     6361 ggcagaaaaa acaaaacatt ttctttgaaa ttttttgtaa ggtgggctaa aaaagaaatc
     6421 agccgctcat acatgccaaa gacagctgct aatgcaccac cgctgactcc tggcaagata
     6481 aatcctgaac cgataaacat tcccttcaca aaccgtaaca tccagtcttt ggttgtgtac
     6541 cccacttctt gattttccat tttttgcctt ttttccacta tttgcgcact ccttctttca
     6601 tttaatttta ttcatgattg aacatttcct gctattttac ttaaagactt tttcataggt
     6661 ctctattatt ttttcattca ttttgacaat atcaggataa aacatgttac ctccattatg
     6721 atagagatag ccgccattgt aaagcaagga ctggatccgc caatatcggt attgtttccc
     6781 ttcctcattt cctaaaagtg ggctcagcac atctttagaa tacgcttctg ctaattctac
     6841 tgtgttttct ccgccatgat tttttacata ggcaatataa tctttcccaa aattatatgc
     6901 ttggacagct gtccataaat cgcatccttg tttctttgct tctactatca tttccgccaa
     6961 atatttgaca ccttgatcaa tactgtcagc tggattatca atgatctcag cttctccatg
     7021 cagactttcc gagctctgca tgagatctgt cccttttcct tttgattcgg tcaatattat
     7081 cgccttgatc aaattctcgt agttttccat atgataattt tttgcagctg ttgttatttc
     7141 tatttgatag ctttccactc gttttaccga acgataagtt cttcctcata aaaacaacac
     7201 cgcaccaata accgtcagaa tgatcatgta aaaaagaata cggataatag ataatcgttt
     7261 tcgcttcatt tcatcaccta gttattttga catctttttt tatcttacta tttctttatc
     7321 ttcttggcta tttcttcctt gatttctgtg tacgccttaa gaaaaaatga attttcctgt
     7381 attttttgac atcttacgaa aatgatcgtt gtttttcagt aaaaacaatt gcccaatgac
     7441 tgatataatc gttatacttt acacagtagg tgattatgtg aaaaaagaaa agaaatatgc
     7501 aaaaatgcca gatgggagcg aaatttacta tgaaaagagc ggtcaaggct tccctctctt
     7561 tttactgcat ggcaatgacg gcagcggccg ctttttttca gaacaggtcc ctgtactcga
     7621 acgctactat accgtgtatc tagtagatag ccgaggacat ggtcgttcga caaacgaagc
     7681 atctatgttg aattttcagt taatggccga ggatctgaat acgatcatgt tgctggaaaa
     7741 aatcgatcag gcagattttc ttggatttag tgatggagca aatctagcac tggtatttgc
     7801 cagcagcttc cctaaaaaag ttcatcgatt gattttaaat tctggaaaca cgcttgttaa
     7861 aggcgttcgt ttttctgcca gagtcatcag taatattcat tatgcatggg tatggttact
     7921 ctctttattt cgcccaagtc ttcgcaaaaa tcttttagtt atcaaacttt tgctgcatga
     7981 catcgggctt acagaaaatg atttaaaaaa aatcaactcc cccactttga tcatcgtggg
     8041 aaaaaaagat gtaatcaagc taaaacattc tttgtatata gctaaaacta taccaaaagc
     8101 ttcctttgtg cttgtgaaag aacaaggaca tgaattagct agaaaagatc ctgaacgctt
     8161 caatcgagaa gttcttcaat ttctttctga aacataacta ttaggaatag aaggtgattt
     8221 atttgttaaa aaataccata caatggctga aaaaccacct aggactgttc aaaacaatct
     8281 ttttgatttc tgtcattgtc attattgtcg gtgaattgat gtccatcggt aaaactctat
     8341 ctatccagca attaggagaa acgtttgcca ctatacctgt atggaaaagc ggactaatgc
     8401 tattgattgg tcttatctct gtgttaccta tggttggtta tgatattatt ttgaatcgga
     8461 tgcttgaaca aaagcagaat cctcgttacc tgtttgagac aagctggctg atcaatacga
     8521 tcaacaatat tgccggtttc ggcgggtttg ttagtgtggg gcttcgctct gaactatatg
     8581 gacaaaagaa agagagcaga aacgtgatcc aagcattatc gaaagttttc ttgtttttga
     8641 tggctggact ttctatttac agtctgcttt cttttttact tgtagtcttc acgccagttg
     8701 ctcctttttt gaagcaatat tggatatggt tgatcggcgg cggtctatat tttcctgtcg
     8761 tctttttatt tactactttc aaaaagaaag gattccttgg tggtacctca cttaaaactc
     8821 aaggacagct attgctagtt tcattgttgg aatggaccgg tgtcctgttt agtttccttt
     8881 ctgttggtta tttaatggaa atccggattg atctgtggca aacaattcca ttattcattg
     8941 ctgcttcagt gataggaatc gtgtctatga ttccaggaga aattgggagt ttcgatgtaa
     9001 tgatgattat cgggttgtcc gctattggtg tgccaagaga aatagtcgtt gtgtggatct
     9061 tgctctatcg cctgttctac tacatcattc cgtttttgat tggaatcgtc tttttcttca
     9121 aaaatatcgg ttccactttt gatcagcgtt attctggtat ccctaaacaa ttagcaacag
     9181 aaattgcgca taaaatcgtg gttgtcttat tatacttttc tggaatcatg ctagtacttt
     9241 ctgccactat cccgcaagct tttacagagt ttcgctggct tcatagtttg aacccattga
     9301 aatttcattt tattatccaa tttcctagta tactattggg atttctttta atcattatgg
     9361 gcagagggat cgcagcacgt gtgaaacgag catatttgcc tacaatcttc ttgattgttc
     9421 tagctttact ttatgtttta ttaagtgatt ttagtttcac accggtggtt ttcttgtcta
     9481 ttctgcttct gatcattctt gcttctaaaa acgaattatt tcgggaacaa ttgatctatt
     9541 catgggagtg gcgtacgatc gatggaatca ttattggtgc actgtccttg ctttacatcg
     9601 ttattggggt atacaatctg ccggatttcc ctcatcggcg tcaccatttt atttcctttt
     9661 tcttatttcc ttctgaaaag atttggtttt cgggattgtt ggccatcatc gctgttagtt
     9721 tcatgatcgt tctatttgtt catttccttc aaggagaaaa gaaacagatt ggcgaagcct
     9781 ttaatgaaga aaaggccctg aagatcctta caacttatgg aggaaactcc gatagtcaat
     9841 tgatcttctt aaaagataag cggatgtttg cctatgaaaa agacggtgaa ccaactgttc
     9901 ttctccaatt cgcttgcttc aataataaat gtattgtaat gggtgatcct tctggtaaga
     9961 aagaagattt tccagaagca atcgaagctt ttatcgaaga aacagatcga ctatgctatt
    10021 taccagtttt ctacgaaaca agcgaagaga ttgttatgat tttgcatgaa ttcggctacg
    10081 atttcattaa gatgggggaa gaagcttatg tagacctgaa tagttttaca acttcaggga
    10141 aaaaaatgaa gggcactcgt gctgtcttga atcggatcga acgagaagga tttacatttg
    10201 atgtcttaca accaccattt tctactgagc aaatgagtac tttcaaaaat atctctgata
    10261 attggttggg ttcacgaaag gaaaaagggt tttctttagg ctttttctct gaagattatc
    10321 tacaaagagc gcctattgca gttgtcaaaa atacccaaga agaaattgtc gcttttgcaa
    10381 cgatcatgcc tacttacacg aacaataaag taggaacgat tgatctgatg cgatatgatt
    10441 ctaaaaaagc accttctggc agtatggatt tcttgttctt gcatttgttt gcctatatga
    10501 aagaagaaca tatccaatgg ttcaatttag ggatggcccc tctttcaaat gtcggaactt
    10561 ccagaaagag ttttttacag gaaaggattg catacttggt ttacgagttt ggttctcatt
    10621 tttattcttt ccatggatta agagaatata aaagcaagta cgctacaaac tgggtctcac
    10681 gttatactct atattctaga gatagttgga ttgcatatgt tatgattgct ttgctgatta
    10741 ttgataatgc gccagttgag cagacacaag gtagaataag tggtttgaaa aaatggatac
    10801 gaagaaagta ttaaaaactg cataccgata gatgaacact ctcatgatga ataaaagaga
    10861 ctgaagtaaa acgaaagttc atgcttctac aatctcttct tatacaattg aagaacaaag
    10921 agcggtccaa acagaaatcc tgtttggacc gctcttttat tttaaacatt tttcattacc
    10981 aaaatattcg agtcgcgttt cttatgcttc gatctctgta acgatacctg aacctactgt
    11041 ccgtccgcct tcacgaattg agaatgtcgt tccgttttca acagcgattg gatggatcaa
    11101 gtcaacatca atcgtcacgt tgtcaccagg catgaccatt tctgtatctt ctggcaatgt
    11161 gattgttcct gtaacatcag ttgtgcggaa gtagaactgt ggacgatagt tgttgaagaa
    11221 tggagtatga cgtccacctt cttcttttgt caacacgtac acttctgctt tgaattttgt
    11281 atgtggagta atggaacctg gtttagcaag tacttgtcca cgttcgatat cgtcacgttg
    11341 gatcccccgt aacaacacgc ctacgttatc cccagcttcc ccgtaatcta acgttttacg
    11401 gaacatttct acacctgtta caactgcttt ttgtgtttca ggtttgatcc ctacgatctc
    11461 tacctcatca ccgacacgaa cagcaccacg atcaatacga cctgaggcaa cagttcctcg
    11521 acctgtaatt gagaagacgt cttccactgg caatagtaat ggtttatctg tatcacgttc
    11581 tggtgttggg atatattcat ctactgtatc catcaattcc ataatagcag cttcagcatc
    11641 tggatcgcct tgcaatgctt ttaatgcgga acctttgatc acaggagtgt catcgcctgg
    11701 gaaaccatat tcgctcaata actcgcgaac ttccatttct accaaatcga tcaattcttc
    11761 atcatcgact aaatcaactt tgttcaagaa aacaatcagg tattttacac caacttggcg
    11821 tgacaataaa atgtgttcac gtgtttgagg catcggaccg tcagttgcag aaacaactaa
    11881 gattgcacca tccatttgtg ctgcccctgt aatcatgttt ttcacatagt ccgcgtgtcc
    11941 tggtgcatca atatgcgcgt agtggcgttt ttctgtttca tattctacgt gtgcggtatt
    12001 gatcgtaatc ccacgttcac gttcttctgg agctgcatca atactagcat agtcttgagg
    12061 atttgctagg ccttttttgc ctaatactgt agtgatcgca gcagttaaag tagttttccc
    12121 atggtcaacg tgtccaatcg taccaatgtt aacgtgtggt ttacttctgt cataatgttc
    12181 ttttgccata atttacaaac ctcctaaata atatagatca ctgaaaaatt tcagctctca
    12241 cctttttatt atagtctttt tttgactaaa tcaaaaagaa tacgtccgac ttactttttg
    12301 taagattcga actaagctta ttatacgtca atattggtca aagtcaacta agagcactcg
    12361 tctgtcttat acgcgaaaaa agtacctttt ataaataaaa aaatgtgttt tttgagaaaa
    12421 acttcaagat agtccttaac tttcttttga agcaataaga acaaaatgag gaaaagttta
    12481 aaattcagct gttgccaaca actttcaata gacgaaatct tctatttcgt taataataag
    12541 gaaagaggtg aagtacatga aaaaattaat tggcgtactt gtcgttttag gcatatttct
    12601 cggcggaagt tttgcagctt atcattattt ttacggcggc gaagcttatt atacaaaaat
    12661 cacgacgaat ggcgaaatgt cagctaccca agcagataac ggagagaaat tcacgactta
    12721 tacctacaag caagcggctt ataataaaga tggtgaaaaa aaacaggtga cgcttcgcga
    12781 agaaagagaa catccattaa agatgaatgc gtatcttaaa ctcaaagtca atcctcgtaa
    12841 aggtgtatta agctgggaag aaataaaggc ttctgaagtg cctaaaaatg cggcagaaaa
    12901 aatcgataat tagtaaatgg tcttaaaatt ttattagata aaaagaggct gggacag
//
LOCUS       DARBET010000074        12734 bp    DNA     linear   BCT 07-NOV-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4027
            SAMEA114569841-rid20112183.denovo.076, whole genome shotgun
            sequence.
ACCESSION   DARBET010000074 DARBET010000000
VERSION     DARBET010000074.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA114569841
            Sequence Read Archive: ERR12203666
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 12734)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12734)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001972618.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001972618.1
            Genome Coverage       :: 132x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/07/2023 13:18:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,041
            CDSs (total)                      :: 2,975
            Genes (coding)                    :: 2,908
            CDSs (with protein)               :: 2,908
            Genes (RNA)                       :: 66
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 67
            CDSs (without protein)            :: 67
            Pseudo Genes (ambiguous residues) :: 0 of 67
            Pseudo Genes (frameshifted)       :: 26 of 67
            Pseudo Genes (incomplete)         :: 28 of 67
            Pseudo Genes (internal stop)      :: 20 of 67
            Pseudo Genes (multiple problems)  :: 6 of 67
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12734
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA114569841-rid20112183.denovo.076"
                     /isolate="VRE4027"
                     /db_xref="taxon:1352"
                     /country="Denmark"
     gene            complement(85..264)
                     /gene="rpmF"
                     /locus_tag="SCJ86_002277"
     CDS             complement(85..264)
                     /gene="rpmF"
                     /locus_tag="SCJ86_002277"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010719637.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L32"
                     /protein_id="HEG1169799.1"
                     /translation="MAVLARRTSKAKKAKRRTHYKLTIKGLNECPNCGEMKKSHHVCA
                     NCGYYDGKDVMSKEA"
     gene            complement(322..891)
                     /locus_tag="SCJ86_002278"
     CDS             complement(322..891)
                     /locus_tag="SCJ86_002278"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF177 domain-containing protein"
                     /protein_id="HEG1169800.1"
                     /translation="MKWSLLELRKYQETPLTFHETLDLKAALMKRDNLILDVAPVEVE
                     GLVSVDKKGYIVHYTVQTTLTVPSTRSLEPVELPMDFSVDEVFMTEEQFQSRDEQMSA
                     DEILLIEGPLLNLAESIEDNILLAIPMRVLTKEEEAATELPKGNDWEVVSEEEYQRRK
                     MEEAENKIDPRLAKLSELFDSKSKDDDNK"
     gene            1077..1979
                     /locus_tag="SCJ86_002279"
     CDS             1077..1979
                     /locus_tag="SCJ86_002279"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344100.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HEG1169801.1"
                     /translation="MNTKDIEYYVQIVKQKNFTKVANYFRVSQPTITYALQRLEKEFD
                     TVLITRDRSHHELIVTPSGKQLFLHAQTILQEISTARAEITQLQKKKLRFGMPPIIGN
                     EYFPKLSSYFIKHELMNQIEITDGGSRDLYGMIRQGRIDLAVLGSTQPIVDQYLETEL
                     LLDKRFMIVVPPTHPLSKQDTVSFKDIADEQFVLLNEHYVHPAAFTKLSKQTHTHPKV
                     VYQSNDLTILKSMVRENVGIGFLAEIAVHPEDHLVCLPLSDEVQPRFLISLAKRTQQI
                     VTPLQEQVVQVIHEFVTAQKNSSS"
     gene            complement(2014..3798)
                     /gene="pyk"
                     /locus_tag="SCJ86_002280"
     CDS             complement(2014..3798)
                     /gene="pyk"
                     /locus_tag="SCJ86_002280"
                     /EC_number="2.7.1.40"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003127858.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate kinase"
                     /protein_id="HEG1169802.1"
                     /translation="MKKTKIVSTLGPASNSVEIISQLIESGANVFRFNFSHGDHEEQL
                     SRMTMVREAVKKTGKDVGILLDTKGAEIRTTVQGTTEADFGRAGYIKFEVGDQTRISM
                     DPEHVGSKEKIAVTYPGLFDDVHVGGHILFDDGLIDMQIIEKDEANRELVVEVKNAGM
                     LGSRKGVNAPGVSISLPGITPKDADDIRFGLDNDIDFIAASFVRKAQDVLDIREILEE
                     KDMTHVQIFPKIESQEGIDNIDEIIKVSDGVMIARGDMGVEIPAELVPMVQKRIIKKC
                     NAAGIPVITATQMLESMQENPRPTRAEASDVANAVFDGTDATMLSGESANGDYPVEAV
                     STMARIDQEAENALSELGTFQLNEFDKTDVTETIGLSVARAAKNLGVKTIVAATESGY
                     TAKMISKYRPDADILAVTFDERTKRGLMLNWGVYPTVAEKPSTTDEMFELAAKKAVEL
                     GFASEGDLILITAGVPVGERGTTNVMKIQMIGSKLLEAQGVGQKSVVANAVIASSAEE
                     AVKKAKDGMVLVVPSTDKEYMPAIEKAAAVIVEEGGLTSHAAVVGIAQDIPVIVGAKG
                     ATTTITEGELITVDSRRGIVYRGETTAI"
     gene            complement(3872..4834)
                     /gene="pfkA"
                     /locus_tag="SCJ86_002281"
     CDS             complement(3872..4834)
                     /gene="pfkA"
                     /locus_tag="SCJ86_002281"
                     /EC_number="2.7.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010746303.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-phosphofructokinase"
                     /protein_id="HEG1169803.1"
                     /translation="MKRIGILTSGGDAPGMNAAVRAVVRKGIYEGLEVYGINYGFAGL
                     VAGDIRRLDVADVGDKIQRGGTFLYSARYPEFATEEGQLKGIEQLKKFGIEGLVVIGG
                     DGSYHGAMALTKRGFPAVGIPGTIDNDIPGTDFTIGFDTAINTVLESIDRIRDTATSH
                     VRTFVIEVMGRNAGDIALWSGVAGGADEIIIPEHDFDMASVAKKIQEGRDRGKKHCLI
                     ILAEGVMGGNEFAEKLSEFGDYHTRVSILGHVVRGGAPSARDRVLASKFGAYAVELLQ
                     EGKGGLCVGIHDNEIVASDIIDTLENNKHKPDLSLYDLNNSLSF"
     gene            complement(5002..8316)
                     /gene="dnaE"
                     /locus_tag="SCJ86_002282"
     CDS             complement(5002..8316)
                     /gene="dnaE"
                     /locus_tag="SCJ86_002282"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319208.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit alpha"
                     /protein_id="HEG1169804.1"
                     /translation="MFFPQLYTITSYSLLQSTIRIREYVQEAKKQGYTSLAITDKDVL
                     YGAPEFYRICVEEGVQPIIGLHLSYTYEEKNYGLLAYAKDEIGYQQLMKLSSRKMIQE
                     KLELAEMTETDHLAFVVPANHSVSEAFNKEDNFFGHWQFLLENVFTRNFYIGLTENTP
                     DQLPEWYQYVIKENYSLCALHPIASLQQEELFALEVMKHIKEGSQIPLENLSASLPLT
                     ANVYLLSEQEMIKRFEKAGLSQAVIGAQRLAESISFEFQFHQKLLPHYPVPNGKQAGE
                     YLRELCYSQVERRITDYTKEYQERLDYELSVIHQMGFDDYFLIVWDVMAFAHRNKIVT
                     GAGRGSAAGSLVAYVLEITDVDPLEYDLLFERFLNPERFTMPDIDLDIPDNRREEVLN
                     YVREKYGQYHMAQIATFGTMAAKMVLRDAARVFGLSQSEANRWSKAIPNQLKITLTQA
                     YKTSHQLVELVQASEKNQLLFKTAVILEGLPRHVSTHAAGVVISDKNLLELVPLQRGS
                     DEAFLTQYTMNEVEAIGLLKMDFLGLRNLSIIDFTLKAVKRVEGYEIRLKEIPLNDAK
                     TLLLFQRGETSGVFQFESAGIRNVLRRLDPENIEDIAAVNALYRPGPMQNIDTFIARK
                     KGKEAIRYPDDSLIPILKNTYGVIVYQEQIMQIASTMAGFTLGQSDILRRAVSKKKKD
                     VLDEEKSHFVSGALQQGYSEKTAATVYEYIEKFANYGFNRSHAFAYSFVGFQMAYLKA
                     HHPGAFFTSLMNSVRHNTSKLKEYIAEARKNKLKLSSPSINQSNSSFELVNKEEIRFG
                     LTAIKGIRRDFVEDILNERKQNGPFQSVDQFLIRIDKRWLKLELLQSLTAVGVFDELS
                     PNRKQLMLDLEGKIQNIVYSGGSLDLLGIMALKEEEVADYTLEERLKLEEEYLGIYLS
                     GHPAEGFDRLRLAKQVQLITDIVPNQPVNLLVMIKEVREIRTKKGELMAFVEGTDSSG
                     EISVTVFPTLYRKYRNLFAVDRVIFVQGRSEISKYNQELQLIGESFADPNQLEAQYPS
                     QTCYLRITEEREDPEIMKQLQVIFQKHPGYIPVVMYHEKTQRKVVLSDAYWVDDSKSI
                     KGQLIYLLGEDNVVFK"
     gene            8547..8732
                     /locus_tag="SCJ86_002283"
     CDS             8547..8732
                     /locus_tag="SCJ86_002283"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290959.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YjzD family protein"
                     /protein_id="HEG1169805.1"
                     /translation="MRYIVTLFWAVVLGQVVGYIGAALTSGTYDFTLTTIISFIAGVI
                     ILLIGAVAPRKETSAHS"
     gene            complement(8794..9990)
                     /locus_tag="SCJ86_002284"
     CDS             complement(8794..9990)
                     /locus_tag="SCJ86_002284"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MFS transporter"
                     /protein_id="HEG1169806.1"
                     /translation="MEINWRKNLVIAWIGCFFTGASISLVMPFIPVYVEQLGTPKDQI
                     ELFSGLAISVTAFASAVVAPIWGNLADRKGRKLMMIRAAAGMTLTMGSLAFVPNAYWL
                     LIMRFWNGILSGYIPNATAMIASQAPREKSGWALGTLSTGAIAGNLIGPSMGGALAQW
                     FGMENVFLITGALLMITTVLTIFLVKEDFHPIEKKDLISTKEIFSKMDHLSILIGLFI
                     TTLILQIGITTISPILTLYIRELSGSTENILFVSGLIVSIAGVSAVFSSPKLGKLGDK
                     IGNQKVLIGGLILSFCCYLPMAFVTTPLQLGILRFILGFSTGALMPSVNTLISKITPQ
                     EGVSRIYSYNQMFSNFGQVLGPMLGSTVARAYNYSAVFIVTSLFVLSNILLSLFNFRK
                     VLRSRL"
     gene            complement(10160..11596)
                     /locus_tag="SCJ86_002285"
     CDS             complement(10160..11596)
                     /locus_tag="SCJ86_002285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290956.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NCS2 family permease"
                     /protein_id="HEG1169807.1"
                     /translation="MEKFFGLKKNNTNVSTEIMAGVTTFFAMSYILFVNPTILSASGM
                     PFQAVFLATIIASIIGTLVMGLFANVPYAQAPGMGLNAFFTFTVVFGLGYSWQQALAM
                     VFICGLINIFITVTNIRKMIIRAIPESLQHAIGGGIGIFVAYVGIKNAGFLSFSADQS
                     AISSSVVEGGKATNVTINGGIVPALANFDNAPILLAVIGLVLTAILVVKNVRGAILIG
                     IVATTVLGIFMGVVDLSSIDWQTNSLGNSFKELGTTFGAAFGSEGMQSLFSDSSKIPQ
                     VLMTIIAFSLSDTFDTIGTFIGTGRRTGIFSKEDEIALEDGRGFKTKMDKALFADAIA
                     TSVGAIFGTSNTTTYVESAAGIGAGGRTGLTSVVVAVLFALSSLFSPLIAIVPNQATA
                     PALILVGVMMMASFADIKWLDMEEALPAFFASIFMGLCYSISYGIAAGFIFYTIVKVI
                     KGKYNQVSVALWIVDILFIINFIILATI"
     gene            11889..12698
                     /locus_tag="SCJ86_002286"
     CDS             11889..12698
                     /locus_tag="SCJ86_002286"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295364.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cof-type HAD-IIB family hydrolase"
                     /protein_id="HEG1169808.1"
                     /translation="MNKKLIAIDLDGTTLNQDSMITSKTKETLKKAINAGHHVSIATG
                     RPFRMSHQFYQQLELTTPMVNFNGALVHIPNQHWDGERETLINREIAFEILSQKKQLN
                     LDFIAAENRDTFFIDSFDFFDEKIFASSRPGEKNLLSPKNLTTNPTSLLVRTDKRFAG
                     AVSAELTRQFGSYVDVRTWGGPTAILEIVSKGIQKAKGVQEIANYLSIDQKDVIAFGD
                     EHNDLELLDYAGWGVAMANGTDQLKGIANDVTPLSNQEDGLAVYLEKLLKL"
BASE COUNT         3803 a         2674 c         2249 g         4008 t
ORIGIN      
        1 attttttatt taccgactac aaaaaaataa acctcaaggc tccatggtta gccttgaggt
       61 tttacatttt ctaattaaaa tgtattaagc ttcttttgac attacgtcct taccatcata
      121 gtaaccacaa tttgcacata cgtgatggct ttttttcatt tcaccacagt ttggacattc
      181 gtttaaccct ttgattgtca atttgtagtg tgtacgacgt tttgcttttt tagcttttga
      241 tgttcttcta gctagtactg ccattttgct acacctcctt gtaaagtctt gtatctaaat
      301 tagacacaat atattccaat cttacttgtt atcgtcgtct tttgatttag aatcaaaaag
      361 ttcagatagt tttgcaagac gaggatcaat tttattctcg gcttcttcca tttttctgcg
      421 ctggtattct tcttcggaaa caacttccca atcattgcct tttggaagtt ctgttgctgc
      481 ttcctcttct tttgtcaata cccgcattgg aatcgccaat aagatgttgt cttcaatcga
      541 ctctgctaaa ttcaataatg gaccttcaat tagcaaaatc tcatctgcag acatttgttc
      601 atctctactt tggaattgtt cttctgtcat gaatacttcg tctacagaaa aatccatggg
      661 cagttcaaca ggttctagtg aacgtgttga aggaactgtc aaagtcgtct gcacagtgta
      721 atgaacgata tagcctttct tatcaactga gaccaaacct tcgacttcca ctggcgcaac
      781 atcaagtatt aaattgtctc gcttcatgag agcggctttc aagtcgagcg tctcatgaaa
      841 agttaacggt gtctcctggt attttctcaa ttccaataaa gaccatttca tttttcatca
      901 ctcctaagca acaaaagcaa ttatacaggg caaaaagcat gttgtcaatg aaattttctt
      961 gacaccttgc agttcataaa aaaacagaaa aatctttatt agaaagcctc tgtttgctat
     1021 catcctcaga atcatttaca ataatcatta ttccattaaa ggagcggatg atcttcatga
     1081 acacgaaaga tatcgaatac tatgtccaaa tcgtcaaaca aaaaaatttt acgaaagtgg
     1141 ctaattattt ccgtgtgagc caacctacta ttacatatgc attacaacgt cttgaaaaag
     1201 agtttgacac cgtacttatt acaagagatc gatcgcatca cgaattaatc gtcacgcctt
     1261 ctggaaagca gctgtttctc catgcacaaa ccattctcca agagatttct actgcaagag
     1321 ctgaaatcac tcagttgcaa aagaaaaagc ttcgttttgg catgcctcct attatcggca
     1381 atgaatactt tcctaaactg tcaagttact ttatcaagca tgaattgatg aatcaaattg
     1441 agattaccga tggaggctct cgggacttat acgggatgat ccgacaaggg aggatcgatt
     1501 tagctgtatt aggctcaaca cagccaatcg tggatcaata tttagaaaca gagttacttt
     1561 tggacaaacg tttcatgatc gtcgtgccac ctacacatcc tctttccaag caggatactg
     1621 tctctttcaa agatatagca gatgaacagt ttgtactatt gaacgaacat tatgtccacc
     1681 ccgcagcatt cactaagctt tctaaacaaa cacatactca tccaaaagtt gtttatcaaa
     1741 gcaatgactt gacgatttta aagagtatgg tccgagaaaa tgtgggtatc ggttttttgg
     1801 cagagattgc tgtccatcct gaagatcatt tagtttgcct ccctctttca gatgaagttc
     1861 agcctcgctt cttgatttca ttggcaaaac gaacacagca gatcgtcacg cctcttcaag
     1921 aacaagtagt ccaagttatc catgaattcg ttacagcaca aaaaaacagc tcttcgtaaa
     1981 agaagagctg tttttatttt ttattaagct aaattagata gctgttgttt cgccacggta
     2041 aacgatgccg cgacgtgaat caacggtaat caattcacct tctgtaattg ttgttgttgc
     2101 gcctttagca ccaacgatta caggaatatc ttgtgcaatt cctacaactg cagcgtgaga
     2161 agtcaatccg ccttcttcaa cgataacagc agctgctttt tcgattgcag gcatatattc
     2221 tttgtcagtt gatggtacaa ctagaaccat tccgtctttt gcttttttta cagcttcttc
     2281 agcagatgaa gcaataactg catttgcaac gacagatttt tgtccaacgc cttgagcttc
     2341 taataattta gaaccaatca tttgaatttt catcacgtta gttgttccgc gttcaccaac
     2401 tggtacacca gcagtgatca agattaaatc gccttctgat gcgaaaccta attcaacagc
     2461 ttttttagct gctagttcaa acatttcatc agttgttgat ggtttttcag caactgttgg
     2521 gtaaacgccc cagtttaaca tcaaaccacg ttttgtgcgt tcgtcaaaag taacagccaa
     2581 gatatctgca tctggacgat attttgaaat catttttgca gtgtatcctg attcagttgc
     2641 agcaacgatt gttttcacgc ctagattttt tgcagcacga gctacagata aaccgattgt
     2701 ttcagttaca tctgttttat cgaattcgtt caattggaat gtacctaatt ctgataatgc
     2761 attttcagct tcttggtcga tacgtgccat tgttgaaaca gcttcaactg ggtagtcccc
     2821 gtttgcagat tcaccagata gcattgttgc atctgtgcca tcaaatacag cgttagcaac
     2881 gtcagaagct tccgcacgtg ttggacgtgg gttttcttgc attgattcta acatttgtgt
     2941 agcagtgata actgggatac cagcagcatt acattttttg ataatgcgtt tttgaaccat
     3001 tggtactagt tcagctggga tttcaacacc catatctccg cgggcaatca taacaccgtc
     3061 agaaactttg atgatttcgt caatgttgtc gataccttct tgagattcga ttttagggaa
     3121 gatttgaaca tgagtcatgt ctttttcttc taagatttcg cggatatcta gaacgtcttg
     3181 tgctttacgt acaaaacttg cagcgataaa gtcgatatcg ttgtctaaac caaaacgaat
     3241 atcgtcagca tcttttggag taatacctgg caaactaata gaaacgcctg gtgcatttac
     3301 ccctttacgt gatcctagca tacctgcatt tttaacttct acgactaatt cacggtttgc
     3361 ttcgtctttt tcaatgattt gcatatcgat caaaccatca tcaaataaaa tgtgtccgcc
     3421 aacgtgaaca tcgtcaaata atcctgggta agtaaccgcg attttttctt ttgaacctac
     3481 atgttcagga tccatagaga tgcgtgtttg atctcctact tcaaatttga tgtatcctgc
     3541 acgaccaaaa tcagcttctg ttgtgccttg aacagttgta cggatttcag ctccttttgt
     3601 atctaaaaga ataccgacat cttttcctgt ctttttaact gcttcacgga ccatagtcat
     3661 acgtgataat tgttcttcat ggtcaccatg agaaaaatta aagcggaaga catttgcccc
     3721 agattcaatc aattgagaaa taatttctac tgagttactt gctggtccta atgtactaac
     3781 gattttggtt tttttcattt aaaaaactct cccattcaat tttgtttatt taaagctgta
     3841 cacagcttaa atagcttggt ttctttcact cttaaaatga aagtgaatta tttagatcat
     3901 atagtgataa gtctggttta tgtttgttgt tttcaagtgt atcaatgatg tcagacgcaa
     3961 cgatttcgtt gtcatggata ccgacacata atccaccttt tccttcttga agcaattcga
     4021 cagcgtaagc accgaattta cttgccaaca cgcggtcgcg tgcgcttggt gctccaccac
     4081 ggacaacgtg tcccaagata gatacacgtg tatgatagtc accaaattct gaaagtttct
     4141 cagcgaattc atttccgccc attacacctt cagccaagat gatcaagcaa tgtttcttcc
     4201 cgcgatcgcg gccttcttgg atttttttag caactgaagc catatcaaaa tcatgttctg
     4261 ggatgatgat ttcatccgct ccgccagcca cacctgacca taaagcgatg tctcctgcat
     4321 tgcgtcccat tacttcgata acgaatgtac gaacatgaga agtcgctgtg tcacgaatac
     4381 ggtcaattga ttctagtact gtattgattg ctgtatcaaa accgattgtg aaatcagtcc
     4441 caggaatatc attatcgatt gttccaggaa tacctactgc tgggaaaccg cgttttgtca
     4501 aagccattgc gccatgataa gagccgtctc ctccgataac cactagacct tcgattccga
     4561 attttttcaa ttgttcgatt ccttttaatt gaccttcttc agttgcaaat tctggataac
     4621 gtgcagaata caagaatgta ccaccacgtt ggatcttgtc acctacatct gccacatcaa
     4681 gacgacgaat atctccagca actaaaccgg caaaaccata attgatcccg tatacttcca
     4741 gtccttcgta aataccttta cgaacaacgg cacgaacagc tgcattcatt ccaggtgcgt
     4801 ctccgccact ggtcaaaatt ccgatgcgtt tcataataaa cattcacctc attaaaatac
     4861 tttatcctaa gtatgactta ggtttacaac catttttgcg ttaaccacga ctaacattct
     4921 aacattattt ttctcacttg tcttgattta cggataaaaa aatgaaaaaa aatgaaagca
     4981 agtagtattt tttaccgtaa gttatttgaa aaccacatta tcttcgccaa gcagataaat
     5041 aagttgcccc tttatactct tgctgtcatc cacccaataa gcatctgaca gaactacttt
     5101 tcgttgcgtt ttttcgtgat acataactac tggtatatat cctgggtgtt tctgaaaaat
     5161 cacttgtaat tgtttcatta tctcgggatc ttcccgttct tctgtaatac gcaaatagca
     5221 agtttgtgaa ggatactggg cttctaactg gttaggatca gcaaagcttt ctccaattag
     5281 ttgcagttcc tgattatatt tgctgatttc gctccgccct tgaacaaaga tcactcgatc
     5341 aacggcaaac aaattacgat atttacgata aagagttgga aaaactgtga cagaaatttc
     5401 tccactactg tcggttcctt caacaaaggc catcagttct cctttttttg tccgaatttc
     5461 tctaacttcc ttgatcatca ctaataaatt gactggttga ttagggacga tatctgtaat
     5521 cagctgaact tgtttagcta aacgaagacg atcaaaccct tctgcaggat gacctgacaa
     5581 ataaatacct aagtactcct cttccagctt caagcgttct tctagtgtat agtcagcgac
     5641 ttcttcttct tttaatgcca tgattcccag caagtcaaga cttccaccac tgtaaacgat
     5701 attttggatt tttccttcga gatcaagcat caattgtttc ctgttaggag ataattcatc
     5761 aaaaactcct actgctgtca gtgattgcag taactctaat ttcagccaac gcttatcgat
     5821 ccgtatgagg aattgatcca ctgactggaa cgggccattt tgttttcttt cgtttaatat
     5881 atcttctaca aaatcacgcc gaataccttt tatcgctgtc agtccgaatc tgatttcttc
     5941 cttattgacc aactcaaaac tggaattact ttgattaatg gacggcgagg aaagtttgag
     6001 tttattttta cgtgcttctg caatatattc tttcaatttt gatgtgttat gccgaacaga
     6061 attcatcagg ctggtaaaga aagcacctgg atgatgggct tttagatacg ccatttggaa
     6121 tcccacgaat gaataagcaa atgcatgtga ccgattgaat ccatagtttg caaatttttc
     6181 gatatattca tatacggtcg ctgctgtttt ttccgaatat ccctgttgta atgctcctga
     6241 tacaaaatgc gacttttctt catctaatac gtcttttttc tttttactca ctgcccgtcg
     6301 cagaatatct gactgcccta atgtaaatcc agccatagtc gaggcgatct gcatgatttg
     6361 ttcctgatag acgatcactc catatgtgtt tttcaaaatc gggatcagtg aatcatctgg
     6421 gtagcgaatg gcttcttttc ctttcttcct ggcaatgaag gtatctatat tttgcatagg
     6481 gcctggtcga tacaatgcat tgactgcggc aatatcttcg atgttttctg gatctaatct
     6541 tcgtaaaacg ttccggatac cagcagattc aaattgaaat actcctgagg tttcgcctcg
     6601 ttgaaacaag agcagcgttt ttgcatcatt tagcgggatc tcttttaatc ggatctcata
     6661 cccttctact cgcttaacag ctttcaaagt aaaatcaata atcgataagt tgcgtagtcc
     6721 taaaaagtcc attttcaaaa gaccgattgc ttctacctca ttcatcgtat actgggtcag
     6781 aaaagcttca tcagaaccgc gttggagcgg aactagttca agtagattct tatcgctgat
     6841 aactacacct gctgcgtgag tcgaaacatg tcttggcaat ccttctaaaa tcactgcagt
     6901 tttgaataaa agttggtttt tttcgcttgc ctgaactaat tctactaatt gatgcgaagt
     6961 tttataagct tgggtcaatg tgattttcaa ttgattgggt attgcttttg accagcggtt
     7021 tgcttcactt tgcgataaac caaacactct agctgcatct ctaagaacca tttttgctgc
     7081 cattgtgcca aatgttgcga tttgtgccat atgatattgt ccatactttt ctcgaacata
     7141 attcaatact tcttcacgtc gattgtcagg aatatccaaa tcgatatctg gcatcgtaaa
     7201 gcgttcgggg tttaaaaagc gttcgaacag caaatcatat tctaatggat ccacatctgt
     7261 gatttctaac acataggcta ctaatgaacc cgcagcagaa ccacgacctg caccggtaac
     7321 gattttgttt ctatgagcaa aagccattac atcccatacg atcaagaaat aatcatcgaa
     7381 tcccatttga tggatgacag ataactcgta gtccaaacgt tcttggtatt cctttgtata
     7441 gtcagtgatt cggcgttcca cttgcgaata gcataactct cttaaatatt ctccagcttg
     7501 ttttccatta ggaactggat aatgcggaag taatttctga tggaactgga attcaaatga
     7561 aatcgattca gctaaacgct gggcgcctat tacagcttgg gaaagacccg ctttttcgaa
     7621 tcgtttgatc atttcttgtt ctgaaagaag atagacattt gctgttagtg gtaaagatgc
     7681 agataaattt tctaaaggta tttggcttcc ctctttgata tgtttcatca cttcaagtgc
     7741 aaaaagttct tcttgttgta aactagcgat gggatgtaat gcacaaagcg aataattttc
     7801 ttttatcaca tactgatacc attcaggcaa ttgatcaggc gtgttttctg tcaagccgat
     7861 ataaaaattt ctagtaaaaa cattttcaag taaaaactgc caatgaccga aaaagttatc
     7921 ctctttattg aaagcctcag aaacagaatg atttgccgga acaacaaacg ctagatggtc
     7981 ggtttctgtc atttctgcaa gttccaattt ttcttggatc atttttcggc tggaaagttt
     8041 catcagctgc tgataaccta tttcatcttt tgcgtacgca agcaagccat aatttttttc
     8101 ttcataagta taactaagat gtaatccaat gattggctgt actccttctt ctacacaaat
     8161 acgatagaat tcaggtgcac cgtataagac atctttatcc gttatggcta atgaagtgta
     8221 gccttgtttt tttgcttctt gtacatattc tcgaatacgt atcgtacttt gtaaaagaga
     8281 ataagaagta attgtataaa gttgtgggaa aaacatcttg cattccgcct tttttttcgt
     8341 taaaattcac tagcgttaaa aggtttgatc cttttacttt cattaagata gcaggcgctg
     8401 aagttgatta ttccacttca tttgcttgct tgtaatctgt aggaatgagt tctttcgcaa
     8461 gaactcaaat tttattgaga tttggtctcg tttctttaca ttcaaagaaa atctggtaaa
     8521 attaagatag aaaagaggtg ttctttatgc gatatatcgt tacattattc tgggctgtcg
     8581 ttctaggtca agtagtcggt tatattggtg ctgcattaac aagcggaaca tatgatttta
     8641 ctttgacaac aatcatttca ttcattgcgg gtgtcattat ccttttgatt ggtgcagttg
     8701 cgccaagaaa agaaacctct gcccattcgt aaaaaaacag ccaagagctt gagatttctc
     8761 aagctctttt ttattttcta ctcctcacca ccattataat ctgctgcgaa gaactttacg
     8821 aaagttgaat aaagaaagaa gaatatttga cagaacaaac aaacttgtca caataaaaac
     8881 agcactgtaa ttataagcac gcgcgactgt cgatcctagc atgggaccta aaacttgtcc
     8941 aaagttgcta aacatctgat tgtagctata gattcggctg actccttctt gaggcgtaat
     9001 cttactgatc agtgtattga cagaaggcat caaagcgcca gttgaaaagc ctaaaataaa
     9061 gcgaagtata cctaattgga gcggtgtagt gacgaatgcc ataggtaaat aacagcaaaa
     9121 tgataaaatg agtcctccaa ttagaacttt ttgattccca atcttatccc ctagttttcc
     9181 tagtttcggt gaagaaaaga cagcggaaac accagctatc gaaacaatca agccgctgac
     9241 aaaaaggata ttttctgtac ttccgctcaa ttcacgaata tataaggtca aaatagggct
     9301 gatcgtagtg attcctatct gtaaaatcaa ggtcgtaatg aagagaccga tcaaaataga
     9361 gagatgatcc atttttgaaa aaatttcttt ggtgcttatc agatcttttt tctcgattgg
     9421 atggaaatct tctttgacta agaaaatggt caataccgtt gtgatcatca acaaagcccc
     9481 tgtgatcaag aatacatttt ccataccaaa ccactgagcc agtgcaccgc ccattgaagg
     9541 tccgatcaga tttccagcaa tcgctccggt agacaatgtt cctaacgccc atccgctctt
     9601 ttctctaggt gcctgtgaag caatcatcgc tgtggcattt ggaatatatc cagacaaaat
     9661 cccattccaa aaccgcatga tcaaaagcca atacgcgttt ggtacaaaag ccaaagatcc
     9721 cattgttaat gtcatccctg ctgctgcacg gatcatcatg agttttcggc cttttcgatc
     9781 agctagattt ccccagattg gtgcaaccac ggcagatgca aaagcagtca cagagattgc
     9841 tagtcccgaa aacaattcga tttgatcttt tggtgttcct agttgctcaa cataaactgg
     9901 tatgaatggc ataactaaac taatacttgc accagtgaaa aaacagccaa tccacgcaat
     9961 cactaaattt tttctccaat taatctccat ctatcatccc tctttcgtct cttttatcat
    10021 acctattttc gaataaacaa gcaacgatct cgccagtaaa aagagaaaaa tcccgcaaag
    10081 gtcattttat atgtaacgtt ctttttatgt aaaacaaaaa gagatcagaa caaatactgt
    10141 tccaatccct tctctttagt taaattgttg ctagaatgat gaagttgata atgaataaaa
    10201 tatcaacgat ccacaaagca acagaaactt gattgtattt tcctttgatt actttcacaa
    10261 ttgtatagaa aataaatcct gctgcaatcc cataggaaat gctatagcac aatcccataa
    10321 agatcgatgc aaagaaggcc ggtaaagctt cttccatatc tagccatttg atgtctgcaa
    10381 aagaagccat catcattact ccaactaaaa tcaatgctgg cgcagttgct tgatttggta
    10441 caatggcgat cagtggagag aataaactac ttaatgcgaa tagcactgcc actactactg
    10501 aagtcaaacc agtacgtcca ccggccccga tcccagctgc agattcgaca taagttgttg
    10561 tattcgatgt accaaagata gcccccacag aagtagcgat cgcatcggca aatagtgctt
    10621 tatccatttt tgttttgaac ccacgaccat cttccaacgc gatctcgtct tcttttgaga
    10681 aaatacctgt gcggcgtcct gtaccgataa atgttccgat cgtatcgaaa gtatcagata
    10741 aactgaatgc gatgatcgtc atcaatactt gaggaatctt ggaagaatca ctgaataaag
    10801 attgcatgcc ttcagaacca aatgctgcac cgaaagttgt tcctaattct ttaaatgaat
    10861 tacctaacga attcgtttgc caatcaatgg aacttaaatc aacaacaccc ataaaaatac
    10921 caaggactgt ggtcgctacg ataccgataa gaatagcccc acggacattt tttacaacta
    10981 aaatggctgt cagtaccaaa ccaatcacag ccaataagat aggagcgttg tcaaagttgg
    11041 ctaacgcagg gacgattcca ccatttatcg taacatttgt cgcttttccg ccttcaacga
    11101 cagagctcga aatagctgac tggtctgctg aaaaagataa gaatcctgca tttttgattc
    11161 cgacatacgc aacgaaaata ccgatcccgc caccaatcgc atgttgaaga ctttcgggaa
    11221 tcgcccgaat aatcattttt cgaatatttg tgacagtaat aaaaatattg attaatccgc
    11281 aaataaacac cattgccaag gcttgttgcc aagaataacc aagcccaaaa acgaccgtaa
    11341 aagtgaaaaa tgcgttcaat cccataccag gtgcctgagc atatggtaca tttgcgaaca
    11401 atcccattac cagcgtacca ataatcgatg caatgattgt cgctagaaat actgcttgga
    11461 agggcatacc tgatgctgac aaaatcgtcg gatttacgaa taagatataa ctcatggcaa
    11521 agaatgtcgt tactcccgcc attatttcag ttgatacatt tgtattgttt ttctttaaac
    11581 cgaaaaactt ttccataaaa agttccactc cctgaatttt tttctgatgg aaaactataa
    11641 taataaaaaa taagtaaaat agggcaaatc catcgaacaa tttcaaatta tatagacgtt
    11701 tcattcgctt ttcaacgttt ttttaacaaa aaaagcgaaa ataatactta acgttcgtgt
    11761 ttagcttatg tttagctatt tatatagcca taactgtagt ttttgttagg aatagctgat
    11821 tgctcttgtt aattacctaa aatatgctag actaaaacaa atgctagttg aggaaggaag
    11881 gacccttatt gaataaaaaa ttaattgcca tcgatctgga cggaactacc ctcaatcaag
    11941 attcgatgat tacatctaaa acaaaagaga ctctaaaaaa agcaatcaat gccggtcatc
    12001 atgtcagtat tgctacagga cgcccattcc gaatgagcca tcagttttac caacaattag
    12061 aattaacaac accgatggtt aattttaacg gcgcattagt acacatccct aatcagcact
    12121 gggatggtga aagagaaacg ttgatcaatc gtgaaatcgc ttttgaaatc ttatcgcaaa
    12181 aaaagcagct aaatttagat tttatcgctg ctgaaaatcg tgatactttt tttattgaca
    12241 gttttgattt ctttgatgaa aaaatttttg cttcttctcg tccaggagag aagaatttgc
    12301 tttctcctaa aaatttgacg acaaatccta catctttgct tgtccgaaca gacaaacgtt
    12361 ttgcaggagc tgtatctgct gaattaacac gtcaatttgg cagttatgtc gatgttcgga
    12421 catggggtgg acctactgcc atcttagaga ttgtttcaaa aggaatccaa aaagcaaaag
    12481 gcgtgcaaga gattgctaac tacctgtcta tcgatcaaaa agacgtcatt gcctttggtg
    12541 acgagcataa tgatttggaa ttgttggatt atgcaggttg gggagtagct atggctaacg
    12601 gtactgacca actcaaaggc attgcaaatg atgtcactcc tttgagcaat caggaagacg
    12661 gtttagctgt ttatctggaa aaactactta aactataata gagaattaga ttccacaaaa
    12721 aagaggttgt gaca
//
LOCUS       DARBET010000075        12116 bp    DNA     linear   BCT 07-NOV-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4027
            SAMEA114569841-rid20112183.denovo.077, whole genome shotgun
            sequence.
ACCESSION   DARBET010000075 DARBET010000000
VERSION     DARBET010000075.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA114569841
            Sequence Read Archive: ERR12203666
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 12116)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12116)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001972618.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001972618.1
            Genome Coverage       :: 132x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/07/2023 13:18:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,041
            CDSs (total)                      :: 2,975
            Genes (coding)                    :: 2,908
            CDSs (with protein)               :: 2,908
            Genes (RNA)                       :: 66
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 67
            CDSs (without protein)            :: 67
            Pseudo Genes (ambiguous residues) :: 0 of 67
            Pseudo Genes (frameshifted)       :: 26 of 67
            Pseudo Genes (incomplete)         :: 28 of 67
            Pseudo Genes (internal stop)      :: 20 of 67
            Pseudo Genes (multiple problems)  :: 6 of 67
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12116
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA114569841-rid20112183.denovo.077"
                     /isolate="VRE4027"
                     /db_xref="taxon:1352"
                     /country="Denmark"
     gene            complement(<1..555)
                     /gene="frr"
                     /locus_tag="SCJ86_002287"
     CDS             complement(<1..555)
                     /gene="frr"
                     /locus_tag="SCJ86_002287"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010752136.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome recycling factor"
                     /protein_id="HEG1169809.1"
                     /translation="MADAIMTEAKEKMKKAAQNLQRELGQIRAGRANASLLDRITVNY
                     YGAPTPLNQMASIQIPEARVLMITPFDKSILQDVEKAIMASDIGISPTNDGNVIRLVI
                     PQLTEERRKELAKDVKKEAENAKIAIRNIRRDAIDEYKKQQKNGDITEDDLRGLEKDV
                     QTLTDNSIKEVDKIAADKEKELLEV"
     gene            complement(558..1280)
                     /locus_tag="SCJ86_002288"
     CDS             complement(558..1280)
                     /locus_tag="SCJ86_002288"
                     /EC_number="2.7.4.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UMP kinase"
                     /protein_id="HEG1169810.1"
                     /translation="MVTPKYQRVVLKLSGEALAGDAGFGIKPPVIKEIIQEIKEVHEL
                     GVEMAIVVGGGNIWRGQIGAQMGMERAQADYMGMLATVMNALALQDTLENVGVPTRVQ
                     TSIEMRQIAEPYIRRKAERHLEKGRIVIFAGGTGNPYFSTDTTAALRAAEIGADVILM
                     AKNNVDGVYSADPKVDANAVKFEELTHLEVIAKGLQVMDSTASSLSMDNDIPLLVFNL
                     NEHGNIRRAILGENIGTTVRGK"
     gene            complement(1416..2297)
                     /locus_tag="SCJ86_002289"
     CDS             complement(1416..2297)
                     /locus_tag="SCJ86_002289"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019722834.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor Ts"
                     /protein_id="HEG1169811.1"
                     /translation="MADVTAKMVKELREMTGVGMMDAKKALVEVEGDMEKAVDLLREK
                     GMAKAAKKNDRIAAEGLASVAVKGNTAAIVEVNSETDFVSKNEMFQDLVKEIAELVAE
                     NKPADMEAAMKIKTDKGTIESDLIEATQVIGEKISFRRFEVVEKDDNAAFGGYLHMGG
                     RIAVLTVLDGTTDETVARDVAMHVAAINPRYVNESQIPEAELEHEKTVLTEQALNEGK
                     PANIVEKMVEGRLKKFKAEIALVDQPFVKDPDMTVEKYVASKGATVKTFVRFEVGEGI
                     EKREDNFVEEVMSQVKK"
     gene            complement(2396..3178)
                     /gene="rpsB"
                     /locus_tag="SCJ86_002290"
     CDS             complement(2396..3178)
                     /gene="rpsB"
                     /locus_tag="SCJ86_002290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875940.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S2"
                     /protein_id="HEG1169812.1"
                     /translation="MAVISMKQLLEAGVHFGHQTRRWNPKMKKYIFTERNGIYIIDLQ
                     KTVKLVDAAYDYMKSVAEEGGVALFVGTKKQAQEAIKEEATRAGQYFVNHRWLGGTLT
                     NWDTIQKRISRLKQINAMEEDGTFEVLPKKEVAGLNKERERLEKFLGGIADMPRIPDV
                     MYIVDPRKERIAVQEAHKLNIPIVAMVDTNCDPDEIDVVIPSNDDAIRAVKLITSKMA
                     DAFIEGNQGEDQVVEEDFTEENTATSIEEIVDVVEGDNSSAE"
     gene            3537..4016
                     /locus_tag="SCJ86_002291"
     CDS             3537..4016
                     /locus_tag="SCJ86_002291"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ArgR family transcriptional regulator"
                     /protein_id="HEG1169813.1"
                     /translation="MRKAQRHLLIKQVIEEFPIRTQEELLNKLGHYGVTATQATISRD
                     IRDLKIVKAPDENGVSRFVLFREKGTPETRTEEEDRLVQMIEDIVLKVERVHFLTIVN
                     TLPDNAHLFASVLDEIKPPSIVSTIAGFDTIMVISENEEDAKRVEEFLHHPTEVSLF"
     gene            4233..5324
                     /gene="phnW"
                     /locus_tag="SCJ86_002292"
     CDS             4233..5324
                     /gene="phnW"
                     /locus_tag="SCJ86_002292"
                     /EC_number="2.6.1.37"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347278.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-aminoethylphosphonate--pyruvate transaminase"
                     /protein_id="HEG1169814.1"
                     /translation="MYKLLTSGPLTTSETVKNAMLIDHCTWDQEYKEITQWIRKKLLD
                     LAQVSEGIYTTVLMQGSSSFGVESVLSSVIRPEDTVLICSNGAYRQRMINMCECLKLN
                     YTVYEVSEHQILQAHVITQLIEENPSISALAMVHSETTSGILNNLEEIAAVTQERDLL
                     FIVDAMSSFGGIPICVAELGIDFLISSSNKCIQGVPGFSFVLANKAELEKRKNVSRSL
                     SLDLYDQYRVMENENGKWRFTSPTHTVLAFQQALKELEAEGGIETRYSRYLANNHVLR
                     QKMTEMGFQPFIQEYQGPFITSFLYPKDWRFDFNHFYQYLKQNGFVIYPGKISDHECF
                     RIGNIGEIYPEDILELTAIIKDYKENVND"
     gene            5317..5445
                     /locus_tag="SCJ86_002293"
     CDS             5317..5445
                     /locus_tag="SCJ86_002293"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326699.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphonoacetaldehyde hydrolase"
                     /protein_id="HEG1169815.1"
                     /translation="MIKTVIFDWAGTTVDFGCMAPVHAFRNAFLEKGIQLTDKEIR"
     gene            5449..5994
                     /locus_tag="SCJ86_002294"
     CDS             5449..5994
                     /locus_tag="SCJ86_002294"
                     /EC_number="3.11.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288430.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphonoacetaldehyde hydrolase"
                     /protein_id="HEG1169816.1"
                     /translation="MGKLKWDHIQQLLSLPSVKKQWIAIYGQLPQEKEVEELYQQFEH
                     YLFKDLVKHSTLKPDTLETIERLRNAGINIGSTNGFNAPMMTIVAETAKKERYSPDFM
                     ATFEDVEGYGRPFPYMIHKNMQYFKNKSVDEVIKVGDTVSDIQEGKNAGVLTVGVIEG
                     SSLMGISYDEYQKLEMGKKIV"
     gene            complement(6448..8139)
                     /locus_tag="SCJ86_002295"
     CDS             complement(6448..8139)
                     /locus_tag="SCJ86_002295"
                     /EC_number="6.1.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019722840.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginine--tRNA ligase"
                     /protein_id="HEG1169817.1"
                     /translation="MNNKDLVAKAVYDAVKDDLTLEQVEQLLENPKSAEHGDVAFPAF
                     SLAKVYRKAPQQIAADLAEKIDSANFEKIEVVGPYLNFFMNKELISKKVLQTVVKEKE
                     HYGDSNIGNQGTVPIDMSSPNIAKPISMGHLRSTVIGNSIGFIMEKIGYQPIRINHLG
                     DWGTQFGKLIVAYKKWGTEEAVKAEPINELLRLYVQFHEVAETEPELNEEARAWFKRL
                     EEGDKEAIQLWQWFRDESMKEFNKIYDLLEVRFDSLNGEAFYNDKMDEIVKLLEEKHL
                     LNEDKGAEIVDLSAYDLNPALIKKSDGATLYITRDLAAALYRKRTYDFKQSLYVVGNE
                     QSYHFKQLKAVLKEMGFDWSDDMHHIPFGLITQGGKKLSTRKGKIVLLEEVLNEAIQS
                     AKEQISEKNPDLENKDAVAKQVGVGAVIFHDLKNDRLNTFDFNLEEVVRFEGETGPYV
                     QYTHARAVSLLEKAGFVPSETADYALNDDTSWEVVKLVQKYPETVLSAGEKYEPSVIA
                     KHAIKLAQAFNKYYAHTKILADDEQKEARLALVYAVTVLLKEDLRLLGLHAPDKM"
     gene            complement(8559..9506)
                     /gene="arcC"
                     /locus_tag="SCJ86_002296"
     CDS             complement(8559..9506)
                     /gene="arcC"
                     /locus_tag="SCJ86_002296"
                     /EC_number="2.7.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313642.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbamate kinase"
                     /protein_id="HEG1169818.1"
                     /translation="MAKRKVVVALGGNAILSTDASAKAQQEALMETAKYLVKFIEQGD
                     ELIISHGNGPQVGNLLIQQQAADSEKTPAMPLDTCVAMTEGSIGYWLQNAMGEVLKEK
                     GIDKDVVSLVTQVIVDENDPSFKNPSKPVGPFYTEEEAKEQMNADSTVTFKEDAGRGW
                     RKVVASPKPISIKEARVIETLVDQGVITVSVGGGGIPVVETATGLEGREAVIDKDFAS
                     EKLAEIIDADLLIVLTGVDNVYVNYQKPDQKKLETVTVSEMKQYIDEKQFAPGSMLPK
                     VEAAIAFVEAKPNAKAIITSLENIENLLASEEGTIIVAD"
     gene            complement(9621..10640)
                     /gene="argF"
                     /locus_tag="SCJ86_002297"
     CDS             complement(9621..10640)
                     /gene="argF"
                     /locus_tag="SCJ86_002297"
                     /EC_number="2.1.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003073325.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ornithine carbamoyltransferase"
                     /protein_id="HEG1169819.1"
                     /translation="MKESVFQGRSLLAEKDFTKEELQYLIDFSEHLKDLKKRGIPHHY
                     LEGKNIALLFEKTSTRTRSAFTTAAIDLGAHPEYLGANDIQLGKKESTEDTAKVLGRM
                     FDGIEFRGFSQKMVEELAEFSGVPVWNGLTDEWHPTQMIADFLTIQENFGTVEGITVA
                     YCGDGRNNMANSLLVTGAILGANMRIVAPKELQPEEEIVKMAEGFAEKSGAQLMITDD
                     VDKGVDGADVLYSDVWVSMGEEDKFEERIKLLKPYQINMEMVEKTHNTDRLIFLHCLP
                     AFHDTNTVYGEQMKERFGITEMEVTDEVFRSKYARQFDQAENRMHSIKAIMAATLGNL
                     FIPRV"
     gene            complement(10731..11960)
                     /gene="arcA"
                     /locus_tag="SCJ86_002298"
     CDS             complement(10731..11960)
                     /gene="arcA"
                     /locus_tag="SCJ86_002298"
                     /EC_number="3.5.3.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338827.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginine deiminase"
                     /protein_id="HEG1169820.1"
                     /translation="MDKPIHVFSEIGKLKTVLLKRPGQEVENLTPDIMDRLLFDDIPY
                     LPIAQEEHDNFAKTLQNEGVETLYLEKLAAEAIDAGNVKEQFLNKMLDESHIASNAVR
                     EGLHEFLLSMETQEMVDKIMAGVRTKDIEVRSSSLYDLSADDDYPFYMDPMPNLYFTR
                     DPSASMGNGMTVNKMTFEARRRESMFTEYILKHHPRFANKGIEVWLDRENPDHIEGGD
                     ELILSDKVVAVGISQRTNAKALEKLARHLFAKNSGFEKVLAIKIPNNRAMMHLDTVFT
                     MVDHDKFTIHPAIQSKDGKMDVFTIEPDGDDIKITHSDNLHETMKAALGLDDLVLIPT
                     GNGDEIVAPREQWNDGSNTLAIAPGVVVTYNRNYVSNELLRSYGIKVLEINSSELSRG
                     RGGPRCMSQPLVREDLK"
BASE COUNT         3309 a         2519 c         2192 g         4096 t
ORIGIN      
        1 aacttccaat aattcttttt ctttatcagc agcgatctta tctacttctt tgatgctgtt
       61 atctgtcaat gtttgtacgt ctttttctaa tccgcgcaaa tcatcttcgg taatatcacc
      121 atttttttgt tgttttttat attcatcgat cgcatcacga cggatgttac gaatcgcgat
      181 ttttgcattt tctgcttctt ttttaacatc tttagctaat tcttttctgc gttcttctgt
      241 cagttgcggg ataactaagc gaatgacatt tccatcattg gttggactga ttccgatatc
      301 acttgccata atagcttttt caacatcttg aagaatgctc ttgtcaaaag gtgtgatcat
      361 taaaacacgt gcttctggaa tttggattga tgccatttgg ttcaatggtg ttggcgcacc
      421 gtagtagttc acagtaatac ggtccaaaag gctagcgttc gcacggcctg cgcggatttg
      481 cccaagttca cgttgaagat tttgtgctgc ttttttcatt ttttcttttg cttctgtcat
      541 aattgcatct gccattttta tttccccctt acagttgttc cgatattttc gcctaagatt
      601 gcacggcgaa tatttccgtg ttcatttaaa ttgaagacaa gcagtggaat atcattatcc
      661 atgcttagtg aacttgcagt tgaatccatg acttgtaatc ctttggcaat aacttccaga
      721 tgagtcagtt cttcaaattt tactgcattt gcatctactt tcggatcagc agaataaaca
      781 ccgtctacat tgtttttagc catcaggata acatctgcgc cgatttctgc cgcgcgtaat
      841 gctgctgttg tgtctgtcga gaagtacggg ttgccggtac cgcctgcaaa gataacgatc
      901 cgtccttttt ctaaatgacg ttctgcttta cggcgaatat atggctctgc gatttgacgc
      961 atttcaatcg aagtttgcac gcgtgtcggt acacccacgt tttccaacgt atcctgtaaa
     1021 gctaaggcat tcataactgt agctaacata cccatgtaat cagcttgagc acgttccatg
     1081 cccatttgtg ctccgatttg tccgcgccag atatttccgc cgcccacaac gattgccatt
     1141 tcaacgccca gctcgtgaac ttctttgatt tcttggatga tttctttgat tactggtggt
     1201 ttgattccaa atcctgcgtc cccagctaaa gcttctccac tcaattttaa gactacacgc
     1261 tgatatttcg gtgtgaccat gttcattcct ccaatatttt ctattgccat tttaccatac
     1321 ttagatgatt tcgacactct aagactggtt tttcttgttt tgcttaaaaa aagggagcgc
     1381 acaaaccgtg cgttcccgtc tgttattcaa cagaattatt ttttcacttg gctcataact
     1441 tcttctacaa agttatcttc gcgtttttcg ataccttcgc caacttcgaa acgtacgaat
     1501 gttttaacag ttgcgccttt tgatgcaacg tatttttcaa cagtcatatc aggatcttta
     1561 acgaatggtt ggtccaccaa agcgatttct gctttgaatt tcttcaagcg tccttctacc
     1621 attttttcaa cgatattcgc tggtttgcct tcgttcaatg cttgttctgt aagaacagtt
     1681 ttttcatgtt ctaattcagc ttctggaatt tgtgattcgt ttacataacg aggattgata
     1741 gctgctacgt gcatagctac gtctcttgca actgtttcgt cagttgttcc atctagaaca
     1801 gtcaatacgg caatacgtcc gcccatatgc aagtatccac caaatgcagc attgtcgtct
     1861 ttttctacaa cttcaaagcg acggaagctg attttttctc cgataacttg agttgcttcg
     1921 atcaagtcag attcaattgt acctttatct gttttgattt tcattgcagc ttccatgtct
     1981 gctggtttgt tttcagcaac aagttctgca atttctttta ctaaatcttg gaacatttcg
     2041 tttttagaaa caaagtctgt ttctgagttt acttcaacga ttgctgcagt gttacctttc
     2101 acagcaactg aagccaatcc ttcagctgca atgcggtcat ttttcttcgc agcctttgcc
     2161 attccttttt cacgtaaaag atcaactgct ttttccatgt cgccttctac ttcaactaat
     2221 gcttttttcg catccatcat tcctacgcca gtcatttcgc gtagttcttt taccatttta
     2281 gctgtaacgt ctgccatgtt taactcctcc tgtgtgtttt tctttttaaa aaaagctgct
     2341 tcaaaggaga gtgaggcttg tcgcccagcc tttgaaacag ctccatcatt caaaattatt
     2401 ctgctgaaga attgtcgcct tcaacaacat ctacgatttc ttcgattgat gttgctgtgt
     2461 tttcttctgt gaaatcttct tcaactactt gatcttcacc ttggtttcct tcgatgaaag
     2521 catcagccat ttttgaagta atcaatttaa cggcacgaat tgcatcgtcg tttgaaggga
     2581 ttactacgtc gatttcatct ggatcgcagt ttgtatcaac catagctacg attgggatat
     2641 ttaatttgtg tgcttcttga acagcaatac gttctttacg tgggtcaacg atgtacatta
     2701 catctgggat tcttggcata tcagcgatac cgcctaagaa tttttcaaga cgttcacgtt
     2761 ctttgttcaa accagcaact tcttttttag gtagaacttc aaaagttcca tcttcttcca
     2821 ttgcattgat ttgtttcaaa cggctgatac gtttttggat agtatcccag tttgtcaatg
     2881 ttccgcctaa ccaacgatgg ttaacgaagt attgtcctgc acgagttgct tcttctttga
     2941 ttgcttcttg tgcttgtttt tttgtaccta cgaataatgc tacgccgcct tcttcagcaa
     3001 cgctcttcat gtaatcataa gctgcatcta ctaatttaac tgttttttgc aagtcaatga
     3061 tgtagattcc gtttctttct gtgaagatat atttcttcat ttttggattc cagcgacgag
     3121 tttggtgacc aaagtgtacg ccggcttcta gtaattgttt catagaaatt actgccatgt
     3181 ttgtttcctc cgatttggtt ttatttttcc tcttcttatc ttctaccttg ccggcaaact
     3241 ttttcaagca cctaccgaca atccaataag aatgtggatt tgctgatgca taaagcaccc
     3301 tcagctatta tacagacaaa agatccgtat cgcaagagca aatatacttt tctgctcaac
     3361 attcaatagt ttacctgaaa gataaaaaga attcaagaaa aacagtagat ttctctttgt
     3421 ttctttatta tcctttttcc ataaaataat tttaggtata aaagacgaat attattgggc
     3481 aatccttctt ttttttgtta tgatacatgt gaaatctttc gtaaaggaga aagaaaatgc
     3541 gcaaagctca aagacatttg ctgattaaac aagtcattga agaattcccg atccgtacgc
     3601 aagaagaact attaaacaaa cttgggcatt atggcgttac cgccacgcag gcaacgattt
     3661 ctcgagatat ccgtgatcta aaaatcgtga aggcacctga tgaaaatggc gtttcccgct
     3721 tcgtattgtt tcgcgaaaaa ggaactcctg agactaggac tgaggaagag gatcggctgg
     3781 ttcaaatgat tgaagacatt gtattgaaag tcgaacgagt ccactttttg acgatcgtca
     3841 ataccttgcc agacaatgct catttatttg catctgtttt agatgagatc aaacctccct
     3901 ctattgtcag tacaattgct ggattcgata cgatcatggt catctcggaa aatgaggagg
     3961 acgcaaaacg agtagaagaa tttcttcacc atccaactga agtatcactt ttttaatcag
     4021 agcacgtatt cctaatatgg aatgcgtgtt tttttatttt caatattttc cttattgaat
     4081 aaacctttac actctttaca aaaatacatc attttttatt caaatttttc aacaccttga
     4141 ttgaatcaca ataatcatca tttatattgg atgttaacaa gagcagcttc ttgctctttg
     4201 tgtatttcaa aaaagaaatg aaaggaaatc atatgtataa attattaact tctggaccat
     4261 tgacgacttc tgaaacagtg aaaaatgcta tgctgatcga tcattgtact tgggatcaag
     4321 aatacaaaga aattacacaa tggataagaa aaaaattatt ggatcttgct caagtatcgg
     4381 aaggaatata cacaacggtc ttaatgcaag gtagcagctc cttcggggta gaatcggtat
     4441 taagttctgt gatcaggcca gaagatactg tactgatttg ttcaaacggc gcatatagac
     4501 aacgaatgat caacatgtgc gaatgtctga aactgaacta taccgtttat gaggtatcgg
     4561 aacatcaaat cctccaagct catgtgatta cacagctgat tgaagagaat ccgtctatct
     4621 ccgcactagc aatggtccat agcgagacta cttccggtat tttgaataat ttggaagaga
     4681 ttgctgctgt cacacaagaa agagatcttt tgtttatagt ggacgcgatg tcaagctttg
     4741 gtgggatacc gatttgtgta gccgaactag ggattgattt tttgatttcc agctcaaaca
     4801 aatgtattca aggtgttcca ggcttttctt ttgtacttgc taataaagcg gaactggaaa
     4861 aacgaaaaaa tgttagccga agtttgtcac ttgatctata tgaccagtac cgcgtgatgg
     4921 aaaacgagaa tggcaaatgg cgtttcactt cccctaccca tacggttttg gcttttcaac
     4981 aagcgctgaa ggaactagag gcagaaggag gaatcgaaac tcgatattca cgttatctcg
     5041 ccaataatca tgtgctacga caaaaaatga cagagatggg gttccagcct ttcattcaag
     5101 aatatcaggg gccattcatc acctcttttc tttaccctaa ggactggcga tttgatttta
     5161 atcattttta tcagtattta aagcaaaatg gatttgtgat ctatcccggg aaaatcagcg
     5221 atcatgaatg ttttcgtatc ggaaatattg gcgagatcta tccggaagat attttggaac
     5281 tgacagcaat tatcaaagac tataaggaga acgttaatga ttaaaactgt aatttttgat
     5341 tgggcaggaa caactgtgga ctttggctgt atggctcctg tccacgcttt tagaaatgct
     5401 tttctagaaa aaggaatcca actgactgac aaagaaatcc gctaaccgat gggaaaatta
     5461 aaatgggatc atatccaaca gcttctttcc ctcccttctg tcaaaaaaca atggatagct
     5521 atttatggac aactacctca ggaaaaagag gtggaggaat tgtatcaaca atttgaacat
     5581 tatctgttta aagatttggt caagcattct accttgaaac ccgacacatt ggaaacgatc
     5641 gaacggttga gaaacgctgg gatcaatatt ggttccacga atggatttaa tgctcctatg
     5701 atgacaatcg tagcagaaac cgcaaaaaaa gaaaggtatt caccagactt catggcaact
     5761 tttgaagacg tcgaagggta tggtcgaccg tttccttata tgatccataa aaacatgcag
     5821 tacttcaaaa acaagtcggt tgatgaagta atcaaagtcg gagatacggt ctcagatatc
     5881 caggaaggga aaaatgccgg agtgttgaca gtaggtgtaa ttgaaggtag ttcgctaatg
     5941 gggatttctt atgatgagta ccaaaaatta gagatgggga aaaagatcgt ttaaaacagt
     6001 ctgttgctct tagattccaa caagccggcg ctgatatttg tgtaaacaat ttaaaagagt
     6061 taacagaata cattttgtac gctgacatga atccagaatg acttttatac aaaataagag
     6121 gttgtgaaaa agctgtcctg tatcaagtaa taaaaacagc caagcaaaaa tagctcctca
     6181 tatttttcgt ttggttaggc ttattaccgc agatgcaagc ttttgaacac cgtttattcg
     6241 aaaaaaaaaa gggagctgtg acacgacttt tgtcacagtc cccttatttg caaacaaaca
     6301 gtgtttaaga gacaacctct ccactaaacg gtgttcattc agctgactct cggtatttgc
     6361 tcaaaaaacg ataaaatttg aaaagctatt ttctcttttg tttctctaat actcggatca
     6421 gaacgctgat ttcacattct ctttttctta catcttatct ggtgcatgaa gtcctagcaa
     6481 acgaaggtct tctttcaata atacagtcac tgcataaact agagctaagc gtgcttcttt
     6541 ttgttcatca tcagctaaaa tcttcgtatg agcatagtat ttgttgaatg cttgtgctaa
     6601 tttgattgca tgtttcgcaa tcactgacgg ttcgtatttt tccccagcag acaagaccgt
     6661 ttcagggtat ttttgaacta atttaaccac ttcccagctg gtatcgtcat tcaatgcata
     6721 gtctgctgtt tctgaaggga caaatcctgc tttttccaac aggctgactg cacgagcatg
     6781 tgtatattgc acataaggtc cagtttctcc ttcaaatcga acaacttctt ccaggttaaa
     6841 gtcaaatgta ttcagtcgat cgtttttcaa atcgtggaaa atgactgccc caaccccaac
     6901 ttgtttagct acagcatctt tgttttccaa atcagggttt ttctcgctga tttgttcttt
     6961 agcagattgg attgcttcat ttaatacttc ttctaataag acgatctttc ctttacgagt
     7021 tgataatttt ttaccacctt gcgtgatcaa accaaatgga atatggtgca tgtcgtcaga
     7081 ccagtcaaag cccatttcct tcaagacagc tttcaactgt ttaaaatgat agctttgttc
     7141 attccctact acatatagag attgtttgaa gtcgtaagta cgtttacggt acaatgcagc
     7201 agctaagtca cgtgtaatgt atagtgtcgc accgtctgac ttcttaatca acgctggatt
     7261 caggtcataa gcagataggt caacgatctc tgcaccttta tcctcgttca gcaaatgttt
     7321 ttcttctaaa agtttgacga tttcatccat cttgtcattg tagaaagctt caccgttcaa
     7381 cgaatcgaaa cgtacttcca gcagatcata gattttattg aattctttca tcgattcatc
     7441 acggaaccat tgccataatt gaatagcctc tttatcgcct tcttccagac gtttgaacca
     7501 agcacgagct tcttcattta attctggttc tgtttcagct acttcatgga attggacgta
     7561 taaacgaagc aattcattga ttggttctgc tttaacagct tcttctgtac cccatttttt
     7621 gtaagcgacg atcagttttc caaactgtgt tccccagtca cctaaatggt tgatgcggat
     7681 tggctgataa ccgatttttt ccataatgaa accgatcgaa ttaccgataa ctgtcgaacg
     7741 caaatgtccc attgagattg gtttggcaat attaggagaa gacatgtcga ttggtactgt
     7801 tccttgattc ccgatgttgc tatccccata atgttctttt tctttcacta ctgtttgcaa
     7861 aacttttttg ctgataagtt ctttattcat gaaaaagttc aagtaaggtc cgacaacttc
     7921 gattttttca aagttggcag aatcgatttt ttctgctaaa tctgcagcga tttgctgtgg
     7981 cgcttttcgg taaacttttg ctaatgaaaa ggctggaaat gcaacatctc cgtgttcagc
     8041 tgattttgga ttttctagta attgctcgac ttgttccagt gttaaatcat ctttgacagc
     8101 gtcatagaca gcttttgcga caagatcttt attattcatc tttattcctc ctaaagtgtt
     8161 cccttgattg ttaaacaaaa aaaatctcta gcgacggcat acttagtatg ccatcactag
     8221 agacgagatt tcccgcggta ccactccaat tgtcctaaaa aaggaccagc tccatctgat
     8281 atcggtagac gaccgtctgt ttaaaacaga attctccaag tgcgcttcgt tgtatcttct
     8341 tcattcggct tccaccactt ccgaattcgc tagccaagaa tgatacaagt actctcttgt
     8401 tcaacaaatc ttagtcttat taaactttct tgaattatat cagaaaaaaa aacgaaaggc
     8461 tagtcgtttc ataaaaaaac aatccagatg gcctaaagga gaagaaatac cacctggata
     8521 gctataaatc atttcgtaaa tacgaaagac ttaacaaatt aatcagctac gataatcgtt
     8581 ccttcttcag aagctaaaag attctcgata ttttctaaag aagtgatgat tgccttagcg
     8641 tttggttttg cttcaacaaa tgcaatagct gcttctactt tcggaagcat gcttcctgga
     8701 gcgaattgct tttcatcaat gtattgtttc atttctgaaa cagtaacagt ttctaatttc
     8761 ttttgatctg gtttttggta attgacatac acattgtcta ctcctgtcaa tacgatcagc
     8821 aaatccgcat cgatgatttc ggctaatttt tctgaagcaa aatctttatc gattacggct
     8881 tctcgtcctt ctaatccagt agctgtttct acaacaggaa tcccaccgcc accaacagag
     8941 actgtgataa ctccttgatc gaccagcgtt tcgatgacac gtgcttcttt aatcgagatg
     9001 ggtttagggg aagcgactac ttttctccat ccacgtccag catcttcttt gaatgtcaca
     9061 gtggagtcag cattcatttg ttctttggct tcttcttccg tgtagaatgg accgactggt
     9121 ttacttggat ttttgaatga aggatcattt tcatccacaa taacttgagt gactaaagaa
     9181 accacatctt tatcgatccc tttttccttc agtacttcgc ccatggcgtt ttgtaaccag
     9241 taaccgatag atccttctgt cattgcaaca catgtatcta aaggcattgc cggtgttttc
     9301 tcagaatcag cagcttgctg ctgaatcaat aggttaccga cttgcggacc atttccgtgg
     9361 gagatgatca attcatctcc ttgttcgata aatttcacta aatactttgc tgtttccatt
     9421 aacgcttctt gttgggcttt tgcacttgca tccgtagaca agatggcatt gccccctaaa
     9481 gcgacaacta cttttcgttt ggccatttca ttcgtccttt cttcagttct accgaatttt
     9541 ctagataaca aaacgagcca gcgttttgtg attttccctc aatgctgact cgttttttcg
     9601 tgagatgaat gtggttatgc ttaaacgcga gggataaaca agttacctaa tgttgctgcc
     9661 atgatcgctt tgattgagtg catacgattt tctgcttggt caaattgacg agcatatttg
     9721 ctgcggaaga cttcatctgt tacttccatt tcagtgatgc caaagcgttc tttcatttgt
     9781 tcaccgtaaa cagtgtttgt atcatggaaa gctggcaagc agtgcaagaa gatcaaacga
     9841 tctgtgttgt gtgttttttc taccatttcc atattgattt ggtaaggttt caacaatttg
     9901 atacgttctt cgaatttatc ttcttcaccc attgataccc atacatcaga ataaaggacg
     9961 tctgctccgt ctacaccttt atcaacatcg tcggtaatca tcaattgtgc accggatttt
    10021 tcagcaaagc cttctgccat tttgacgatt tcttcttcag gttgtaattc ttttggtgca
    10081 acgatacgca tattcgctcc taaaattgca cctgtgacta ataaagagtt agccatattg
    10141 tttcgaccat ctccgcagta agcaacagtg ataccttcta ctgttccaaa gttttcttgg
    10201 atagttaaaa agtcagcgat catttgtgtt ggatgccatt catcagtcaa gccattccaa
    10261 actggaacac cagagaattc tgctagttcc tctaccattt tttgactgaa tccacggaat
    10321 tcgattccgt caaacatacg tcctagtact ttagctgtat cttctgtgga ttctttttta
    10381 cctaattgga tatcatttgc acctagatat tcaggatgtg cacctagatc gatcgctgca
    10441 gtagtaaatg cagaacgagt acgtgtagat gttttttcaa ataaaagcgc aatatttttt
    10501 ccttctagat agtgatgtgg gataccacgt tttttcaagt ctttcaagtg ttctgaaaaa
    10561 tcgatcaaat attgtaattc ttcttttgta aagtcttttt ctgctaataa gcttcttcct
    10621 tggaatactg attctttcat attaaacatt ctcctttaga ttgtttttat ttaagattaa
    10681 agaggaaatc ccgacggcag ctgacagtaa gactgccgtc aggaaacaaa ttacttcaag
    10741 tcttcacgga caagtggttg gctcatgcag cgtggaccac cacgacctcg tgacaattca
    10801 cttgaattga tttctaacac tttgatgcca tagctacgaa gcaattcgtt cgatacataa
    10861 tttcggttat aagtgactac aacacctgga gcgattgcca atgtgtttga tccatcattc
    10921 cattgttctc gaggtgcaac gatctcatct ccattaccag ttgggatcag tacaagatca
    10981 tctaatccaa gagcagcttt catcgtttcg tgtaagttat ctgaatgagt aatcttgata
    11041 tcatcaccat ctggttcgat tgtaaagaca tccatcttac cgtctttact ttgaattgct
    11101 ggatggatag tgaatttatc gtgatctacc attgtaaata cggtatccaa atgcatcatt
    11161 gcacggttgt tcggaatttt gattgccaac actttttcaa atcctgaatt ttttgcgaat
    11221 aggtggcgtg ctaatttttc taaagctttc gcattagttc gttgagaaat cccaacagct
    11281 actactttat cgcttaagat caattcgtct ccgccttcga tatgatctgg attttcacga
    11341 tccaaccata cttcgattcc tttatttgca aatcttggat gatgtttcaa aatatattct
    11401 gtaaacatcg attcacgacg acgtgcttca aaagtcatct tattgactgt catcccattt
    11461 cccattgatg cggaagggtc gcgtgtaaag tacaagtttg gcattgggtc catgtaaaat
    11521 ggatagtcat cgtctgctga cagatcatat agactgctag aacgtacttc gatatctttt
    11581 gtacgaaccc cagccatgat tttatctacc atttcttgcg tttccattga tagaaggaac
    11641 tcatgaagac cctctcttac agcattcgat gcaatatgag attcatccaa cattttattc
    11701 aagaattgtt ctttgacatt gcctgcgtca atcgcttctg ccgctaattt ttccaagtaa
    11761 agagtctcga cgccttcatt ttgcaatgtc ttcgcaaagt tgtcatgttc ttcttgagca
    11821 atcggaagat atggaatatc atcgaacaac agacgatcca taatatctgg tgtcaaattt
    11881 tccacttctt gtccaggccg tttcagtaaa actgtcttta atttccctat ttcagagaaa
    11941 acgtgaatag gtttatccat gttgtgttgc ctccttcttt ttgattacat gtataggata
    12001 tcctcctttt gtaatcactt tcataatttt gtctcattca aaatgagaca ttcctcacat
    12061 taatattcga taattaaata attaaacgtt tttctatttt taagcgtaat ttattc
//
LOCUS       DARBET010000076        12075 bp    DNA     linear   BCT 07-NOV-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4027
            SAMEA114569841-rid20112183.denovo.078, whole genome shotgun
            sequence.
ACCESSION   DARBET010000076 DARBET010000000
VERSION     DARBET010000076.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA114569841
            Sequence Read Archive: ERR12203666
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 12075)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12075)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001972618.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001972618.1
            Genome Coverage       :: 132x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/07/2023 13:18:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,041
            CDSs (total)                      :: 2,975
            Genes (coding)                    :: 2,908
            CDSs (with protein)               :: 2,908
            Genes (RNA)                       :: 66
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 67
            CDSs (without protein)            :: 67
            Pseudo Genes (ambiguous residues) :: 0 of 67
            Pseudo Genes (frameshifted)       :: 26 of 67
            Pseudo Genes (incomplete)         :: 28 of 67
            Pseudo Genes (internal stop)      :: 20 of 67
            Pseudo Genes (multiple problems)  :: 6 of 67
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12075
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA114569841-rid20112183.denovo.078"
                     /isolate="VRE4027"
                     /db_xref="taxon:1352"
                     /country="Denmark"
     gene            complement(163..1242)
                     /locus_tag="SCJ86_002299"
     CDS             complement(163..1242)
                     /locus_tag="SCJ86_002299"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006908283.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS30 family transposase"
                     /protein_id="HEG1169821.1"
                     /translation="MTQNHDNTVSRKGKHLTYSERCQIAILKKENYSNRQVADVLGCV
                     PQTINNEIKRGTVTQLNRQNQNGKVYDYYHDVYDADAGQAAYEKNRLNCGRRPLWADT
                     DAFIEWADDMMLLEKWSPDVVVGFARKHELFDPAIIPGTTTLYQWIDRGIMKTKNMDL
                     LEKLSRKPKSSRHGNRPNKRVLGKSIEERPEAVGTRKTFGHWEVDTVVGNKTKSDAVL
                     LTLVERQTRFEVILKLAGKDAESVNQAVRQLRERAGAYFGQLFKTVTSDNGSEFAGLH
                     DALQDVANIYFSHPYASWERGTSENQHKLIRRFIPKGSPIGNVSENQILRIQRWMNDY
                     PRKILGYATPHDTFVQAFRQERLTA"
     gene            complement(1592..2440)
                     /locus_tag="SCJ86_002300"
     CDS             complement(1592..2440)
                     /locus_tag="SCJ86_002300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338311.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MurR/RpiR family transcriptional regulator"
                     /protein_id="HEG1169822.1"
                     /translation="MSVSIRLQIKTMYNDFSPKEQAIADYILENPSKVSHSPISNLAS
                     ELGIADSTFFQFTKKLGYNGFKDFKMAMLIQENDLSAISIHENIQKTDTELTMAQKVF
                     ESSISTLNDTKKLLQQKDLKKAATIINESKRVYFFGVGGSEIVATDAYHKFLRSPIAT
                     SHSTDYHIQLMEASLLTEKDCAVLISHTGQSKETIHIAETVRKTGAKTIVITSQANSS
                     LAKLGDVVFISISEETEFRSEALASRISQLSILDSLYVILMFYNEKESRRSIAKVRHT
                     ISEIKE"
     gene            2439..3506
                     /gene="gnd"
                     /locus_tag="SCJ86_002301"
     CDS             2439..3506
                     /gene="gnd"
                     /locus_tag="SCJ86_002301"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321675.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="decarboxylating 6-phosphogluconate
                     dehydrogenase"
                     /protein_id="HEG1169823.1"
                     /translation="MRIPHFHNKIIIFSVLINVKDTRRVGKNQTKNIFVKSIYKKIFL
                     YNEGIRNGGAIMEIGVIGLGKMGLNLALNLKEHHHKVQGLDISSTAVSQARENDIPAY
                     QEISEFLATFEQKKIIWLMLPAGEITENMLRKLFPKLGPGDIIIEGGNSYYKDSIRRA
                     EEFQKNEIGYLDCGTSGGIEGARHQACLMIGGDKETFLSVEQLFKDVAIERGYLYAGA
                     SGSGHFLKMIHNGIEYGMMQAIGEGFQLVQKSEYAFDLEQVARVWNHGSVIRGWLMEI
                     MEDQLNEHPNLSNYRGIVSASGEAKWTVETALEMAVPVPVIATSLFMRNLSQEEDSFS
                     AKVVSALRNGFGGHEIVEKGE"
     gene            3508..4509
                     /locus_tag="SCJ86_002302"
     CDS             3508..4509
                     /locus_tag="SCJ86_002302"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296641.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxyacid dehydrogenase"
                     /protein_id="HEG1169824.1"
                     /translation="MPDYHIAIVNSSSFGRVFPHHLERLKSVGDVHFFTVDQEIPGKE
                     LAELLKGFNIIIASVTPFFRRDFFENKDELLLISRHGIGYNNIDLKAAKEHQTIVSII
                     PALVERNAVAENNITNLLSMLRRTTQAQARVKDDRWEDRAEFVGRTLFNKTVGVIGAG
                     NTGSGVIEILRNGFRCDVLAYDPYKSDLYIESFGAKKVELQELLEQSDIICLCANLTE
                     ENYHMISDKEVDQMKENVYLSNSARGALVDEEAMINGLKSGKIAGYATDVLEEEPGRQ
                     DHPYLAFEQVVITPHISAYTRECLEAMGEKCVRDVEQISQHLLPERSVQTKSNYIKG"
     gene            4506..4790
                     /locus_tag="SCJ86_002303"
     CDS             4506..4790
                     /locus_tag="SCJ86_002303"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321677.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alcohol dehydrogenase"
                     /protein_id="HEG1169825.1"
                     /translation="MMKKMVNGLKVKTGPQFYLYEEGGISKVSDLLKSYGAKRVLVTH
                     GTVSWEKALPKLVFLNDETIQFFYHRYSGECSYAEARRIATIIKKMKSIS"
     gene            4823..5620
                     /locus_tag="SCJ86_002304"
     CDS             4823..5620
                     /locus_tag="SCJ86_002304"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-containing alcohol dehydrogenase family
                     protein"
                     /protein_id="HEG1169826.1"
                     /translation="MGYSANLARIDFGVIPTLASNCAPWTPLAVMYKENGQAEGKTEH
                     FYRQAAFFITDPQLVIDSPVPYFIAGLADTLAKWYESELILEQDFLQDESFLKLAQDT
                     AKICKEEILNHSAKAIQDMAQRKLTSEFKQLSEIIFAVAGLVGGLGDKYARNAAAHAM
                     HDAISKYIPESHRFLHGEKVAYGIFYQLALEGRWEKIDELYPLYRELELPVSLEQMGL
                     FPKDKQIIDKMAEFIDSKEKVHLIPIEVTQKSLIQSMMELEGYIRKI"
     gene            5634..6467
                     /locus_tag="SCJ86_002305"
     CDS             5634..6467
                     /locus_tag="SCJ86_002305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HEG1169827.1"
                     /translation="METLSVVQSLLIALWVAAVMSRWLGGGATLTLRFSPLMTGLVVG
                     LIMGEVAQAMIVTAALQMIYMGGFSPGGSMPAEPSIAAAIAVPVALLGDLSPEAAIAV
                     AVPVGLLGSYLYQFRFFINTFLGKYTDKAVEDLNTKAMARSIILYPTVASFLLFVPLV
                     FIALYLGAPLIADVIKALEGTVVIHILEVVGGGLAAIGIATTVYVIGRKDYLVFFFLA
                     YFMSVVLKSLDITMVTYAIFGIIIALIFVQSQKGKQVQPATSNNNSASFDDEDDYDDG
                     F"
     gene            6485..7342
                     /locus_tag="SCJ86_002306"
     CDS             6485..7342
                     /locus_tag="SCJ86_002306"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296647.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS system mannose/fructose/sorbose family
                     transporter subunit IID"
                     /protein_id="HEG1169828.1"
                     /translation="MAEIMDNQTGAPEEITKKDVTKAYLRWHFANEIPHSFERYLAPS
                     LLYGMMPILKKLYKDEDSLKAAYKRQLLFFNTQLSWGGGVITGLMSSMEQQRAEEEYE
                     EKEILMQDDLMYNTKAGLMGALAGIGDAIDSGTVQYIFIAIAVPWAQQGSPLGALFPF
                     ICFGLYQVLLGVFFARQAFTMGRNATGLMQNTGVQTAIEMLSVLGLFMMGVLAGNYVK
                     VESSLKFAISGREFVIQEILDQIVPGILPLAVVMGVYLFYTKKGLKVTQALLWLTGIL
                     IVLAGIGIL"
     gene            7364..7873
                     /locus_tag="SCJ86_002307"
     CDS             7364..7873
                     /locus_tag="SCJ86_002307"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296648.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HEG1169829.1"
                     /translation="MGVVNLARVDERLIHGQVMVTLSQKSGVNSIFVVDEVVAKDKFM
                     RDLYKNAGSRTGQKTIVITPEKAKFYWDEYQFKEYNCILIAKTVQTIYDLVKHGIPME
                     ELNIGGIAQKNPDTDLLVTKSVYLNKSDAEKLKEMNEQYGVKNIYFQATPSAPKTGLK
                     DVLNKFDLS"
     gene            7910..8311
                     /locus_tag="SCJ86_002308"
     CDS             7910..8311
                     /locus_tag="SCJ86_002308"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346685.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEG1169830.1"
                     /translation="MKKNELFRDWEFRYRYIYRKRRTKKSKQRFLSALVSDIYSMRTD
                     VTVIAYDTLAYRSKNIYVGDIEKAEKVICTYYDTPVHALGSYFMFDWKDQRKKTIYSI
                     LLSFILLFSLGWWGMMIYNKNPHHVFDLLSV"
     gene            8423..8776
                     /locus_tag="SCJ86_002309"
     CDS             8423..8776
                     /locus_tag="SCJ86_002309"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321678.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEG1169831.1"
                     /translation="MLEMIRTIDDPSVAYAFVDEGCYGEKGLDSVRSGMKKEAILFYL
                     DSVGADTPLQFSGNYFSNKEQWLKQVDKLKEKNVNYIFSARKKQAQFFYLTKTDLRGK
                     TFNWQNANQIIALFR"
     gene            8810..9253
                     /locus_tag="SCJ86_002310"
     CDS             8810..9253
                     /locus_tag="SCJ86_002310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339224.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS fructose transporter subunit IIA"
                     /protein_id="HEG1169832.1"
                     /translation="MLEIMIVTHGKLSEGLKDAAEVIVGTSSHIETMCLESGEDIQTF
                     GEKVKEKLTMINTAEPMLVFVDLISASPYNQALLAVNELTEEKKAATYVIGGVNLPML
                     LEAINHQMLHTPIEEAVEQIVKQGKDNLTVWHASAEEKKLDEDDF"
     gene            9270..10355
                     /gene="uxuA"
                     /locus_tag="SCJ86_002311"
     CDS             9270..10355
                     /gene="uxuA"
                     /locus_tag="SCJ86_002311"
                     /EC_number="4.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannonate dehydratase"
                     /protein_id="HEG1169833.1"
                     /translation="MKWGFRWYGEKGDSIPLKYVRQIPGINGIIGTLLTKMPGDVWEI
                     SEIEELKASVESQGLELLGIESVAIHDAIKAGTDERDYYIDNYIRTIKNLSRCGVRLI
                     CYSFKPIFGWAKTDLFNENNDGSYSLLYDQKIVDQMEPGEMYQLIHSQSKGFKLPGWE
                     EERLKKFAQLEKLYEGMTEEELFENLTYFLEKVIPVCEKADVKMAIHPDDPPWELFGF
                     PRVTKNLGDLKKILNAVDSPYNGLTLCTGSLGANPENDLVEIIHEVGSRIHFVHFRNV
                     EFLGERKFKETAHPSTEGSLDMYHIMKALVQEGFDGPIRPDHGRTVWGEVAMPGYGLY
                     DRAMGLTYMQGLYEAISKEKPLGEHYE"
     gene            10348..11061
                     /locus_tag="SCJ86_002312"
     CDS             10348..11061
                     /locus_tag="SCJ86_002312"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374653.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family phosphatase"
                     /protein_id="HEG1169834.1"
                     /translation="MNKIKLFIFDMDGLLLETGRLAYRAYVKSAQKYDYEMRKEVYYL
                     LTGQTEMAIREQMGILYGEDVPYIKWREAINQYKEKIVKEDKRVYTKKGAEEILSFAK
                     ERGIHTIVASSNTRVKVEMYLKMENLYALFDQIISGDDVKKGKPEPEIFLKACSKMNI
                     PPSEALVFEDSIAGIEAARRAGILSFLIKDHLIGLPDHNGKHKLKVDVSLYESNKADY
                     EFYDLHQAKRFLESKGLYL"
     gene            complement(11268..11417)
                     /locus_tag="SCJ86_002313"
     CDS             complement(11268..11417)
                     /locus_tag="SCJ86_002313"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEG1169835.1"
                     /translation="MTFYNKDIQYRVTLDTDLNLFVVFDKENTNHVATGVTIEQAVQE
                     LKKSA"
     gene            11813..12013
                     /locus_tag="SCJ86_002314"
     CDS             11813..12013
                     /locus_tag="SCJ86_002314"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003234247.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cold-shock protein"
                     /protein_id="HEG1169836.1"
                     /translation="MNNGTVKWFNADKGFGFITGEDGNDVFAHFSAIQGDGFKTLEEG
                     QAVSYDVEDGQRGPQATNIVKA"
BASE COUNT         3846 a         2065 c         2678 g         3486 t
ORIGIN      
        1 catggtgacc cggttgctcg ttaccaccaa ccgtctaatt tttctaatcg agtgggccct
       61 tgacaatgag ctgaggcatg gtagtctccc gactggctgg ccccggccag ctttccagac
      121 acaagccatg ccgagggagg ctcgttgtca agggcccacg tgtcaagccg tcaaccgttc
      181 ctgcctaaat gcctggacga acgtgtcatg cggcgttgca tagcccaaaa tctttcgcgg
      241 gtaatcgttc atccaacgct gaatccgcaa gatttggttt tcgctcacgt tgccgattgg
      301 gctgcccttg gggatgaaac ggcgaatcag cttgtgttgg ttttcgctcg tcccgcgctc
      361 ccaggaagca taggggtggc tgaagtagat gtttgccaca tcctgcaagg catcgtgcag
      421 tcccgcgaac tccgagccgt tgtccgaggt aaccgtcttg aacagctggc cgaagtacgc
      481 accagcccgt tctctcagct gtcggaccgc ttggttgacc gattcggcat ccttcccggc
      541 cagcttcaga atgacctcga agcgtgtctg ccgttccacc aacgtcagca gcacggcatc
      601 cgacttggtc ttgtttccta cgaccgtgtc gacttcccaa tggccgaagg ttttccgggt
      661 accaaccgcc tccgggcgct cttcaatgga tttccctagc acccgtttat tcgggcggtt
      721 gccatgacgg gacgatttcg gtttgcggga cagtttttct agcaggtcca tattcttcgt
      781 tttcatgatg ccacggtcga tccactgata gagggtggtg gtcccaggga taatggccgg
      841 gtcgaacaat tcgtgttttc tggcaaagcc caccaccacg tccggggacc atttttccaa
      901 cagcatcatg tcgtccgccc attcaatgaa ggcgtccgtg tcggcccata agggccgtct
      961 gccgcagttc aagcggttct tctcgtaggc cgcctgcccg gcatcggcgt cgtagacgtc
     1021 atgataatag tcgtagacct tcccgttctg attctggcgg ttaagctgag taacggtccc
     1081 gcgtttgatt tcgttgttta tggtctgcgg cacgcagccc aggacatccg caacctgacg
     1141 gttggaatag ttttcctttt tcaggatggc gatttggcaa cgttctgagt aggttaggtg
     1201 ttttcccttg cgtgaaactg tgttatcatg gttttgcgtc atgtgaattc atccttgtct
     1261 attgagagtt gtggtaactt caatataaca tggaattcac gtggcgtttt tttatttcca
     1321 aatggctaag ttaattataa aatccgccaa caaaaataat aaaaaaaaga aaagataaat
     1381 tttttactac tgtatactaa ctttgttatc gcatctaaca agtaactcga ataataccaa
     1441 tacaaccttc tccaaagatg cgatgacata cgttctaacc caccgactat tgatgttctg
     1501 taccaccctt gcagaaccaa aaaagccact accaaatatt tggtagtggc tttttttgtt
     1561 ttatggattt ttcgcttttt ctgttatggt gctattcttt gatttcagaa atagtatgtc
     1621 gaactttagc gatagaacga cgtgattctt tttcgttgta aaacatcaaa atcacatata
     1681 gagagtccaa aatgcttagt tgagaaatac gggaagctaa agcttcagat cggaattctg
     1741 tttcttcaga aatagaaata aaaacgacat cgccaagttt agcaagggaa gaattagctt
     1801 ggctagtaat aacgattgtt tttgctccag tttttcttac ggtttcagca atatggattg
     1861 tttcttttga ttgccctgtg tgagaaatca aaacagcaca gtctttttcc gtcaataaag
     1921 aagcttccat caattgaata tggtaatccg tgctgtgaga agttgcgata ggggaacgca
     1981 aaaatttatg ataagcgtca gttgctacaa tctctgaacc gccaacaccg aaaaaataga
     2041 cacgtttaga ttcattgata atagtagccg ctttttttag atccttttgt tgtaacaact
     2101 tttttgtgtc atttaaagtt gaaatactag actcgaacac cttttgcgcc atagtgagct
     2161 cagtatctgt tttctgaata ttttcgtgga tagaaatagc agatagatca ttttcttgga
     2221 tcaacatggc cattttaaaa tctttaaatc cattataacc aagttttttt gtaaattgaa
     2281 aaaaagtaga gtcagcaatg cccagttcac ttgctaaatt gctaatagga ctatgactta
     2341 ctttgcttgg attttctaaa atataatcgg cgatagcctg ttcttttggg ctgaagtcgt
     2401 tgtacattgt tttgatttgt agtcttatgg agacgctcat gagaattcct cattttcata
     2461 ataagattat cattttttcc gttctcatta atgttaaaga tacaaggaga gtaggaaaaa
     2521 atcaaacaaa aaatattttt gtaaaatcta tttacaaaaa aatttttttg tataatgagg
     2581 gtataagaaa tggaggcgct atcatggaaa ttggtgtaat tggtttggga aaaatgggat
     2641 tgaatttagc tttaaactta aaagagcacc atcacaaagt acaaggactc gacatttcaa
     2701 gtacagctgt tagtcaggca agagaaaatg acattcctgc ttatcaagag atctctgaat
     2761 ttctagctac ttttgagcag aaaaaaatca tctggttaat gcttcctgct ggagagataa
     2821 ccgaaaacat gttaagaaaa ctttttccaa aactcggacc aggagacatt attattgaag
     2881 gcggaaactc ttattataaa gattcaatcc gtcgagcaga agagtttcag aaaaatgaaa
     2941 tcggttactt ggattgtggg acttctggag gaatcgaagg agcacgacat caagcctgct
     3001 taatgattgg aggagataaa gaaacttttc tatccgtgga acaattattc aaagatgtag
     3061 caattgaaag aggatatctg tatgctggtg catccggaag cggacacttc ttgaaaatga
     3121 tccataatgg aatcgaatat ggaatgatgc aagcaatcgg tgaaggattc caacttgtac
     3181 aaaaaagtga gtatgctttt gacttggaac aagtcgcacg tgtttggaat catggttcgg
     3241 tgattcgcgg ctggctgatg gaaatcatgg aagatcagtt aaacgaacat cccaatttaa
     3301 gtaattatcg gggaatcgtt tctgcatcag gtgaagcaaa atggacggtt gaaacagcgc
     3361 tagaaatggc tgttccagtt ccagtaattg caactagttt gttcatgagg aatctttcac
     3421 aggaagaaga tagtttttca gcaaaagtag tttcagcact aagaaatggg tttggcggac
     3481 atgaaatagt tgaaaagggt gagtaaaatg ccagattatc atatagcaat cgttaattcc
     3541 agcagtttcg gaagagtctt tccacaccac ttggaaagat taaagtcagt tggagacgtt
     3601 catttcttta ctgttgatca agagattcca ggaaaagaac tagcggagtt acttaaagga
     3661 ttcaatatca ttattgcaag tgttacgcca ttttttcgac gagatttttt tgaaaacaaa
     3721 gatgaactgc tattgatttc ccgacatggt attggttaca acaatattga tttaaaagca
     3781 gcaaaagaac accagacaat cgtatctatc atacctgctt tagttgaacg aaatgctgta
     3841 gcagaaaata atataactaa tttgttgtcc atgcttcgac gcacgacaca agcacaagca
     3901 agagtcaagg acgatcgatg ggaagatcgg gcggagttcg tcggtaggac gctgtttaac
     3961 aaaacagttg gtgtgattgg tgcaggaaat accggaagcg gagtaatcga aattttgcgt
     4021 aacggttttc gctgtgatgt tctagcttat gatccttaca aatcagattt atacatcgaa
     4081 agctttggtg caaaaaaagt agaacttcaa gaattactgg aacaatcaga tatcatttgt
     4141 ttatgtgcga atttgacaga agagaactat cacatgattt ctgataaaga agtcgatcag
     4201 atgaaagaaa atgtttatct atctaatagt gcaagaggcg ctctagttga tgaagaagcg
     4261 atgattaatg gtctgaaaag cggaaagatc gcaggatatg ccacagatgt tttagaagaa
     4321 gaaccgggac gtcaagatca tccgtattta gcgtttgaac aagtagtgat tacgccacat
     4381 atatcggcct acacaaggga atgtttagaa gcaatgggtg aaaaatgtgt cagagatgtc
     4441 gaacagatca gtcagcacct tcttcctgaa cgttctgtcc agacaaagag caactatata
     4501 aaaggatgat gaagaaaatg gtgaacggtt taaaagtgaa aacaggccct caattttatt
     4561 tatatgaaga aggtggtatt tcaaaagttt ctgatctctt gaaatcatac ggcgcgaaaa
     4621 gagtactggt cacccacgga acggtatcat gggaaaaggc gctacctaaa ttagtatttt
     4681 tgaatgacga aacgattcaa tttttttatc atcgttattc tggagaatgt agttatgctg
     4741 aagccagacg aatagctaca atcatcaaaa aaatgaaatc gatttcgtag ttggcgttgg
     4801 cggaggaaaa ctgaccgatc tagtgggtta tagtgccaat cttgcaagaa tcgatttcgg
     4861 agtcatccct actttagcta gtaattgtgc tccatggaca cctcttgctg tcatgtataa
     4921 ggagaatgga caagcagaag ggaaaacaga acacttttac agacaagctg ctttttttat
     4981 aacagatccc caattagtga ttgattcccc tgttccatat ttcattgccg ggttagcaga
     5041 tacactagca aaatggtatg aatctgagct catcttagaa caagattttt tgcaagatga
     5101 atcatttttg aagctagcac aagatactgc gaagatctgc aaagaagaga ttttgaatca
     5161 ttctgctaaa gcaatccaag atatggcaca aagaaaactg acatcggagt tcaaacagct
     5221 ttcagagatt atctttgctg ttgctggttt agttggagga ttaggagata aatacgcaag
     5281 aaatgcggca gcacatgcta tgcatgacgc tatcagtaaa tatattccgg aaagccatcg
     5341 ttttctacat ggagaaaaag tagcgtatgg gattttctat caactggcac tagaaggtcg
     5401 ctgggaaaaa atagacgagt tatatccttt gtatcgggaa ttagaactgc cggtttctct
     5461 agaacaaatg ggattatttc cgaaagataa acagataata gataaaatgg ctgaatttat
     5521 tgattcgaaa gagaaagtgc atttgattcc tatagaggta acacagaaat cactgataca
     5581 gtcaatgatg gaactggaag gttatatcag aaagatctga aggaggagga cacatggaaa
     5641 cattaagtgt agtacaaagt ttgttgattg ctttatgggt agccgcggtt atgtcccgct
     5701 ggttaggtgg aggagccacg ttgaccttac gtttttctcc tttgatgact ggattagtcg
     5761 tcgggttgat catgggggaa gttgcacaag cgatgattgt tacagccgct ttgcaaatga
     5821 tttatatggg gggtttttct ccaggaggat caatgccagc tgaaccatct attgcagcag
     5881 ctatcgcagt accagtcgca ttattagggg atctgtcgcc agaagcagcg attgccgttg
     5941 cggtgccagt cggattgctc ggaagttact tgtatcagtt tcgttttttc atcaatactt
     6001 ttttaggtaa atatacagac aaagctgtag aagatttgaa tacaaaagct atggcccgat
     6061 caattattct ttatccaacg gttgcctcat ttttattatt cgttccgctt gttttcattg
     6121 ctttatattt aggggcgcca ttgatcgctg atgtaatcaa agctttagag ggaacagtcg
     6181 ttatccacat tcttgaagta gtaggtggag ggttagcggc aatcggtatc gcgacaactg
     6241 tatacgttat cggtcgaaaa gattatctgg tctttttctt ccttgcttat tttatgagtg
     6301 ttgttttgaa atctttagat attacgatgg ttacttatgc catttttggg attattatcg
     6361 ctttgatctt tgtccagtcg caaaaaggga aacaagtaca gcctgccaca agcaataaca
     6421 attcagcatc ttttgatgac gaagatgact atgatgacgg attttaattt aggaggaatg
     6481 ggaaatggct gaaataatgg ataatcaaac aggtgctcct gaagaaatca cgaaaaaaga
     6541 tgtaacaaaa gcctatctga gatggcattt tgcaaacgaa attcctcact cttttgaacg
     6601 ttacttggcg ccatcactac tatacggaat gatgccaatc ttgaaaaaac tttataaaga
     6661 tgaagactct ttaaaagcag catataaacg gcagttactg ttcttcaaca ctcaattaag
     6721 ctggggtggc ggtgtgatta ctggcctaat gtcttctatg gaacagcaaa gagcagaaga
     6781 agagtatgaa gaaaaagaga tcttgatgca ggatgacttg atgtataaca cgaaagcggg
     6841 attgatgggg gcactagcag gaatcggaga tgcaatcgat tcaggaaccg tccaatatat
     6901 ttttatcgca atcgcggtac cttgggcaca acagggaagt cctttaggcg cactttttcc
     6961 ttttatctgc tttgggttgt atcaggtttt actaggggta tttttcgcaa gacaagcttt
     7021 cactatgggc cgtaatgcta caggcctaat gcaaaacact ggtgttcaga cagcgattga
     7081 aatgctatca gttttagggt tgtttatgat gggggtgctt gcgggaaact atgtaaaagt
     7141 agaatcttca ttgaaatttg cgatatccgg acgtgagttt gttatccagg aaattcttga
     7201 tcaaatcgtg ccagggatat tacctttagc tgtcgtaatg ggtgtgtact tgttctatac
     7261 gaaaaaagga ttgaaagtca cacaagcatt attatggctg actggaatat taattgtgct
     7321 agcaggaatc ggtattttat aaaaagaagg gaacgtgaat aagatgggtg tagtcaattt
     7381 agcgcgtgtg gatgagcgtt tgatccatgg gcaagtaatg gtaacgttat ctcaaaaaag
     7441 tggagtgaat tccatttttg ttgtagacga agttgtagca aaagataaat ttatgagaga
     7501 tctttacaaa aatgcaggaa gccgtacagg acaaaaaaca attgtcatta caccagagaa
     7561 agctaaattc tattgggatg aatatcaatt taaagaatac aattgtattt tgatcgcaaa
     7621 aacagtccaa acgatttacg acttagtaaa acacggaatc cccatggaag aattgaatat
     7681 tggagggatt gctcaaaaaa atcctgatac agatctttta gtgacgaaat ctgtttattt
     7741 aaataaatca gatgctgaaa aattgaaaga aatgaatgaa caatatggcg taaagaatat
     7801 ttattttcaa gccactccat ctgcacctaa aacaggatta aaagatgtat tgaacaagtt
     7861 tgatttgtca taaacaaatg cttagaaaaa agataaagag ggcgaaaaaa tgaagaagaa
     7921 cgagctgttt cgtgattggg aatttcgtta tcgctatatt taccgtaagc gccgtacaaa
     7981 aaagagtaaa cagcgttttc tttcagcatt agtatcagat atttacagta tgagaacaga
     8041 tgtgacagtc attgcttacg atacgctagc ctaccgatca aaaaatattt atgttggaga
     8101 tatcgaaaaa gcagagaaag ttatctgtac ttattacgat acacccgttc atgcattagg
     8161 aagttatttc atgtttgatt ggaaagacca aaggaaaaaa acaatctact ctatattatt
     8221 gtccttcatt ttgttgttca gtttaggttg gtggggaatg atgatatata acaaaaatcc
     8281 tcatcacgta ttcgatctgc tatccgtata aacaggtatc actgtactag catttgggag
     8341 ctactttttc ttattgggaa aagcagctcg cggatggcct agcagacaaa cttttattcg
     8401 aaatacttca tccatattaa cgatgctaga aatgatccgt acgattgatg atccgagtgt
     8461 agcgtatgcg tttgttgatg aaggctgtta tggagaaaag ggcttagaca gcgtgagatc
     8521 gggtatgaaa aaagaggcta ttctttttta tctagactct gttggggcag atacaccgct
     8581 acagttttcg ggaaattatt tttcaaataa agaacagtgg ctgaaacaag ttgataagtt
     8641 gaaagaaaaa aacgtcaatt atatttttag tgcaagaaaa aagcaagcgc agttcttcta
     8701 tttgaccaaa acagatttac gagggaaaac atttaattgg caaaatgcca atcagattat
     8761 tgctcttttt cgataagtaa aaagtgcaaa acaaggagtg agaagactaa tgttggaaat
     8821 catgatcgtt acacatggta agttgagcga aggactgaaa gatgcggcgg aagtaattgt
     8881 cgggacatcg agccatattg aaacaatgtg cttagagtct ggggaggaca tccaaacatt
     8941 cggagagaag gtcaaagaaa aattaactat gatcaacaca gcagaaccca tgctagtatt
     9001 tgttgatcta attagcgcaa gtccgtacaa tcaagcgctc ttggcagtca atgaactgac
     9061 tgaagaaaag aaagcagcca cttatgtcat agggggcgtg aatctgccaa tgttgttaga
     9121 agcaatcaat catcagatgc ttcacacacc gatagaagaa gcggtggaac aaattgtcaa
     9181 acaaggaaaa gataatttaa ctgtttggca tgcttcagcg gaagaaaaaa aattagatga
     9241 ggacgatttt tagaaggaag gctgaaaaga tgaagtgggg atttagatgg tatggtgaaa
     9301 aaggggattc gatcccatta aaatatgttc gccaaatacc gggaataaat gggataattg
     9361 gaactttgct gactaaaatg cctggagatg tttgggagat ttcagaaatt gaggagttga
     9421 aagcatctgt tgagagccaa ggactggaac tgcttggaat tgaaagtgta gcgatccacg
     9481 atgcaatcaa ggcagggaca gatgaacgag attactatat tgataactat atacgaacaa
     9541 tcaaaaactt gtctcgatgt ggggttcgat tgatttgtta tagcttcaaa ccaatttttg
     9601 gatgggcgaa aactgatttg tttaatgaaa acaacgatgg aagctattct cttttatacg
     9661 atcagaaaat cgtcgatcag atggagccag gagaaatgta tcaattgata cacagccagt
     9721 caaagggatt caaattgccg ggttgggaag aagaacggtt gaaaaaattc gctcagctgg
     9781 aaaaactgta tgaaggaatg accgaagaag aattatttga aaatctgact tattttttag
     9841 aaaaagtgat tccagtgtgc gaaaaagcag atgtaaaaat ggctatccat ccagatgatc
     9901 cgccatggga actttttggt tttcctagag tgacgaaaaa tcttggcgac ttgaagaaaa
     9961 ttctaaatgc agtagattct ccatataatg gactaacgct gtgcacagga tctttagggg
    10021 caaatccgga aaacgattta gttgaaatca tccatgaggt aggttcgcgc atccattttg
    10081 tccacttcag aaacgtggag tttttaggag aaagaaagtt caaagaaacg gcacatccct
    10141 ctacagaagg ctcccttgat atgtatcata ttatgaaagc gttggtgcaa gaagggttcg
    10201 acggtcctat acgccctgat catggtagaa ccgtgtgggg tgaagtggct atgcctggat
    10261 atggcctgta tgatcgtgca atgggactca cttacatgca aggtctatat gaggcgataa
    10321 gtaaggaaaa accactggga gagcattatg aataaaatca agctcttcat ctttgatatg
    10381 gatggccttt tattagaaac tggacgttta gcttatcgtg cttatgtaaa atcagcacaa
    10441 aagtatgatt atgaaatgcg taaggaagtc tattatttac taactggcca aacagaaatg
    10501 gcgataagag aacaaatggg cattttgtac ggagaagatg ttccatatat caaatggcga
    10561 gaagcaatca atcaatataa agaaaaaatc gtgaaagaag ataagagagt atatacgaaa
    10621 aaaggagcag aagaaatttt atcttttgca aaagaaagag gaatccatac aatcgtggct
    10681 tcgtctaata cacgggtaaa agtagaaatg tatttaaaaa tggaaaatct atatgccttg
    10741 tttgatcaaa tcatttcagg ggatgatgta aaaaaaggaa agccggaacc agagatcttt
    10801 ttgaaagctt gttcaaaaat gaatattcct ccaagtgaag cacttgtatt tgaagactcg
    10861 attgcaggga tcgaagcagc aaggcgagcg ggcattttgt cttttctcat caaagatcat
    10921 ttgatagggt tgcccgatca taatgggaag cataagttga aagtagatgt aagtttatat
    10981 gaatccaata aagcagacta tgaattttat gacctgcatc aagcaaaacg atttttagaa
    11041 agtaaagggt tgtatctcta ataaagagga ctattttcta gtcctcttta tttattgtcc
    11101 tgcacgactg agggaacaga tgaaaaagaa gtaatttttt attttgaagt atctacctta
    11161 tttgtagtga acaatttaga acaaacacaa aacgcatcta ctgttatcta ccagtagatg
    11221 cgtttaaaga gaagaacgag aatcgtccat atgagggttt ccactcctta tgcagatttt
    11281 tttaattctt gcactgcttg ttcgatcgta acacctgtcg ctacatgatt ggtattttct
    11341 ttgtcaaaaa cgacaaatag attcaaatct gtatccaagg ttactcgata ttgtatatct
    11401 ttgttataga atgtcatagc cattctcctt tctttaaata cttgctgaaa ggcaacgaac
    11461 gcgttgataa cgaaattcca tttataagta taccaccaat tgaaaggttt tcatagttaa
    11521 gaaattttaa agtcaaaagt tgcagataaa agcaaataaa caagaaaaca aaaagtgaat
    11581 caatcgcggt tattcaactt tttcttcgat tcgaagacaa tagaagacaa tgaattttat
    11641 atttcattgc attatccttg tgttattaga aaaagcatga tatattaaat gagtaatcta
    11701 ttttgaaacg taatttgagc gatatatcta catagataaa actccttttg cctatcaaat
    11761 atttattgca acgaaaaaac gtacattgat acgtatcagg aggaatttat ttatgaataa
    11821 cggtacagta aaatggttca acgcagacaa aggttttggc tttatcactg gtgaagatgg
    11881 aaatgatgta tttgcacatt tttcagctat ccaaggtgac ggcttcaaaa ctttagaaga
    11941 aggccaagca gtgtcttacg atgttgaaga tggtcaacgt ggccctcaag caacaaacat
    12001 tgttaaagct taataaaaat tgacttttaa ccgtcttttc gaaagaaaag acggtttttt
    12061 tgtacgaaaa aattc
//
LOCUS       DARBET010000077        11678 bp    DNA     linear   BCT 07-NOV-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4027
            SAMEA114569841-rid20112183.denovo.079, whole genome shotgun
            sequence.
ACCESSION   DARBET010000077 DARBET010000000
VERSION     DARBET010000077.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA114569841
            Sequence Read Archive: ERR12203666
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 11678)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11678)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001972618.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001972618.1
            Genome Coverage       :: 132x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/07/2023 13:18:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,041
            CDSs (total)                      :: 2,975
            Genes (coding)                    :: 2,908
            CDSs (with protein)               :: 2,908
            Genes (RNA)                       :: 66
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 67
            CDSs (without protein)            :: 67
            Pseudo Genes (ambiguous residues) :: 0 of 67
            Pseudo Genes (frameshifted)       :: 26 of 67
            Pseudo Genes (incomplete)         :: 28 of 67
            Pseudo Genes (internal stop)      :: 20 of 67
            Pseudo Genes (multiple problems)  :: 6 of 67
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11678
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA114569841-rid20112183.denovo.079"
                     /isolate="VRE4027"
                     /db_xref="taxon:1352"
                     /country="Denmark"
     gene            complement(105..629)
                     /locus_tag="SCJ86_002315"
     CDS             complement(105..629)
                     /locus_tag="SCJ86_002315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008268881.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEG1169837.1"
                     /translation="MSEEVGIILREAVPGDAKDILLMMGQVNKETEFLVLDEAELLLP
                     PETLEEELDYIYESNNNLLLLAIYEGTIIGTASVKADSQFRLSHVGEVGISILQEYWG
                     MGLGTLMLEEIISWAKEMGVLFRLELDVQVRNERAVHLYRKMGFQIEAVMPRGARTDL
                     GEFLDVYKMSYLIE"
     gene            complement(622..1095)
                     /gene="tsaE"
                     /locus_tag="SCJ86_002316"
     CDS             complement(622..1095)
                     /gene="tsaE"
                     /locus_tag="SCJ86_002316"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323522.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     ATPase subunit type 1 TsaE"
                     /protein_id="HEG1169838.1"
                     /translation="MIELSGLTMTEQFAEAIGHSAMPGDNLILTGDLGAGKTTLTKGI
                     AQGLGITQMIKSPTYTIIREYSQGRIPLYHMDIYRVAASGADLGLDEYFEGDGLSVVE
                     WGNLLEEALPEDYLELILEKSDTDLEKRYVKLISYGEQSETFKQRILEKWAENHE"
     gene            complement(1218..2198)
                     /gene="pta"
                     /locus_tag="SCJ86_002317"
     CDS             complement(1218..2198)
                     /gene="pta"
                     /locus_tag="SCJ86_002317"
                     /EC_number="2.3.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate acetyltransferase"
                     /protein_id="HEG1169839.1"
                     /translation="MELFDSLRFKIVRRGIKIVFPEATDARILGAAARLKAEELVEPI
                     LVGNEEEIRNAAHARGIKTSSFTIISPDNYDKWDEMVDTFVERRNGKASKEQAEKILK
                     DVNYFGTMLTYMGIADGMVSGAIHSTGDTVRPALQIIKTKPGISRTSGAMIMVRGRDQ
                     EKYVFSDCAINVNPTAQELAEIAVDSAKTAELFDIEPKVAMMSFSTKGSAKSPEVDKV
                     VEATKIAKKLAPQYMIDGELQFDASYVPSVAQLKAPDSEVAGQATVFVFPELQSGNIG
                     YKIAQRFGNFEAIGPILQGLNKPVSDLSRGANEEDVYKLSIITAAQTLID"
     gene            complement(2351..3031)
                     /locus_tag="SCJ86_002318"
     CDS             complement(2351..3031)
                     /locus_tag="SCJ86_002318"
                     /EC_number="3.2.2.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uracil-DNA glycosylase"
                     /protein_id="HEG1169840.1"
                     /translation="MKEIIHNSWQEVLSSEFSKDYYLHLREFLKKEYASQKIHPDMYH
                     IYEALELTPYEEVKVVILGQDPYHGENQAHGLSFSVQPGVKIPPSLRNIYKELYDDLG
                     IAPVQHGNLVSWAKQGVLLLNTVLTVREGQAYSHRGKGWERLTDTIIEKLNEREKPIV
                     FILWGKPAQEKIKMIDKSRHIIITSPHPSPLSASRGFFGSKPFSKTNDALLALGEEPI
                     DWQLPETV"
     gene            3157..4026
                     /locus_tag="SCJ86_002319"
     CDS             3157..4026
                     /locus_tag="SCJ86_002319"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002308385.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cof-type HAD-IIB family hydrolase"
                     /protein_id="HEG1169841.1"
                     /translation="MIKLIASDMDGTLLDAHMSVSPENAEAIRFANDAGVEFMVATGR
                     NYQEARAALDEVGIDCAMITLNGAQVFDKEGNSLFTVSIPNNQAISVLDILDANGIYY
                     EVATNDGLYSENQAKRIESFASMVATHLPHLTYKMAIAMASAKLELLHITYVDSIRAI
                     LEEKNLEVLKIICFHTEGPAVLGPVGKQISLLGDLAVTSSGQNNIEVNHKNAQKGIAV
                     AHVAHERGISLEEVMTIGDNFNDVSMLQVAGVSFAMGNAEIEVKDYAKYTTDTNLESG
                     VGKAILRAIAEDL"
     gene            4052..4567
                     /locus_tag="SCJ86_002320"
     CDS             4052..4567
                     /locus_tag="SCJ86_002320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEG1169842.1"
                     /translation="MSEFFIQEKQLGRATRTIVKNEKGHSLFLMVGRWGTKGDALSLY
                     AMNGDLVAHIKQISLTFGTRFELYKEFEKVGTMQKIFNWPGDFYYIRRLHWTVQGDIY
                     NHQYQIHHFNQQIMKMDKATLFSGDYYVLDVSNDEDAPVCICIAAVLDYWLYRKDKSR
                     DKPVSYRLNLS"
     gene            complement(4614..5207)
                     /locus_tag="SCJ86_002321"
     CDS             complement(4614..5207)
                     /locus_tag="SCJ86_002321"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306107.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEG1169843.1"
                     /translation="MLEWRKGEETMIRFARKEDAPEIAPLILVILKDMELPFLKQTGE
                     QKTLEILEEAITDPNYRYSYSRGIVEEIDGKVAGVAYGYTDEEESIIDQPLVPILEKY
                     QLDASTRLFIDKETFPNEWYLDSISVSEDFRGQGIGSRLLEALPKLAKKANRSVIGLS
                     VDEKNPKAKKLYERHDFKVVGQRMISGHLYDHMQKNI"
     gene            complement(5232..5411)
                     /locus_tag="SCJ86_002322"
     CDS             complement(5232..5411)
                     /locus_tag="SCJ86_002322"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEG1169844.1"
                     /translation="MEKMKCPNCGKKFAYEEVNNVVEHNDKEMPVVCPYCRTEAARIV
                     THGYFITEKIEDFLK"
     gene            complement(5488..5997)
                     /locus_tag="SCJ86_002323"
     CDS             complement(5488..5997)
                     /locus_tag="SCJ86_002323"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326315.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEG1169845.1"
                     /translation="MKKMMFGVAALIVIGITGMTGLLNAAFHMDDISDQEYRISLKKA
                     AAIVKNETKEHNISAIHFAVEKDLENPTNATYEYQFFCEGQVIAVNPSTGKTTVRNDT
                     EKAAEHLFDIEKTEEFKQPQAAMKEAVSYYGKKAKVKEWTLLVKDERPYYKVRLIDGE
                     QDRQVWISA"
     gene            complement(6093..6473)
                     /locus_tag="SCJ86_002324"
     CDS             complement(6093..6473)
                     /locus_tag="SCJ86_002324"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334905.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HEG1169846.1"
                     /translation="MNLTTIHHIAIIVSDYQKSRDFYVNKLGFEVIRENYRKERDDYK
                     LDLKLGSAELEIFGIANAPQRPNYPEACGLRHLAFKVDDIEETIAELQSLGIESEPIR
                     EDTFTGKKMTFFFDPDGLPLELHE"
     gene            6803..7552
                     /locus_tag="SCJ86_002325"
     CDS             6803..7552
                     /locus_tag="SCJ86_002325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinamide mononucleotide transporter"
                     /protein_id="HEG1169847.1"
                     /translation="MKSYFQFLAHQLKGWPQQNYYLFFFSLGCQVMTLVNSPITWLSV
                     ITFIGTTLGVLCILSINAAKSVNGILGILSAICFIIVGFSAKNYLSIGEQIAYMITLD
                     IPVLLSKEWNHNMASKIRKFTGKSWFIAIISTFLVYLVSGFLIGRYTDDPRPWVDAIA
                     FSISLTAGIISFLRYNNQYYWWLASGLAQMVLWYISFTHGSASLAMFVNSSVYLLNDV
                     LAFTVSPWYNQKERERMQKMEEEYYNAQQHA"
     gene            complement(7736..9013)
                     /locus_tag="SCJ86_002326"
     CDS             complement(7736..9013)
                     /locus_tag="SCJ86_002326"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336015.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEG1169848.1"
                     /translation="MEEWQSVFEEWFPKEISKSYPIKISKQYTSSQRWEIYAKLTKKQ
                     RELVDKHRRYLISSRFMEEHYLAATDWVFSDFKINPFFRTKRSQQKLYCECGRELKVQ
                     YIVKSPKTGKILKLGINHFADHLHVSPTVAASIHQGMTKVDLALDELLWLKQKNIDFP
                     EGLWQKYCFVLYQNRRMKQPYLPDIKLAQRLAEFRQVEMPIYIADYQALENEIKKISE
                     HINGQSKKRQIKKELFDDFAEELVKDVEEFLINYRAFLRKDWQSIVYEEVPVHPNAYF
                     ETFISVLRKTKRQRTPEVTAQMEYFAKNQRFIQPKIYLFIWKQYCRYGFTEGFFDSIP
                     RIVRNGFLKVLRKEREAIQSADKKDRTVSKEKWQLVVKDIQSGNVQETIDKWKGKHYR
                     FTEAQKQALEYYQKLEESLRFNDEARKYLKELL"
     gene            complement(9092..9736)
                     /gene="pcp"
                     /locus_tag="SCJ86_002327"
     CDS             complement(9092..9736)
                     /gene="pcp"
                     /locus_tag="SCJ86_002327"
                     /EC_number="3.4.19.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734749.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyroglutamyl-peptidase I"
                     /protein_id="HEG1169849.1"
                     /translation="MKVLVTGFDPFGGDKVNPAYEAVKKMPDEISGAEIIKVEVPTVF
                     EKSSQVVKEAIQQHQPDVVICVGQAGGRSAVSFERVAINLAEARIPDNEGNQPFDTAL
                     EENGPAAYFTSLPIKAMTKNVQDHGLPAYISYTAGTFVCNDIMYRLLHLIETQFPAIR
                     GGFIHVPFSPDQVIDRPVGTPSMSLEDIASSLTYAVEAAVENKEDISGNAGTTH"
     gene            complement(9935..10879)
                     /locus_tag="SCJ86_002328"
     CDS             complement(9935..10879)
                     /locus_tag="SCJ86_002328"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF979 domain-containing protein"
                     /protein_id="HEG1169850.1"
                     /translation="MDFFTNPDILLGDKLLEILYIVMGLVLIYTGVRNLTDKTNPHRY
                     GSAFFWTVLGVVIAGGRWLPAIVNGGLIFAMTIPAIAKKVSKGESRLPSKEYMEKMSD
                     KLGMKIFIPALSIGVFAILFALFTNLGALVGVGVGVFAAMLILMFFSRDNRPSTFLDD
                     AADMLGTVGPLSMLPMLLASLGAVFTSAGVGTVISNAVGTIVPEGNVNIGIIIYALGM
                     VIFTVIMGNAFAAITVMTVGIGAPFVFSHGANPALIGMVALTCGFCGTLLTPMAANFN
                     IVPVAMLEMKDKYGVIKNQLFIALFMLVFQIAYMILFK"
     gene            complement(10881..11567)
                     /locus_tag="SCJ86_002329"
     CDS             complement(10881..11567)
                     /locus_tag="SCJ86_002329"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010719881.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF969 domain-containing protein"
                     /protein_id="HEG1169851.1"
                     /translation="MENYLSLLGVLIVILGFAFKLDSILIVMVALVVTALTGGLGIQG
                     MLELLGTSFVNNRGMAIFIIIMLATGTLERNGLKESAASLIKRFKKVSAGMIVDIYGI
                     FRMIFAAFNVSFGGVAGFVRPIILPMALGTIESKGLEVHPKHEEELKGMASAMENVCW
                     FFGQVLFIGGAGALLVQSTLKDLGYDVTLGQLALVEVPVAITALVVASVYFYLKEKKL
                     AKKYYPGKGQ"
BASE COUNT         3334 a         2470 c         2050 g         3824 t
ORIGIN      
        1 tattcaaaaa aaatggactg cgacacgact tttgtcacag tcccttcgtg tgtttttctg
       61 ccttctcaaa aggatgacag tttctcactc ttttcctatt tcttctattc aatcaaataa
      121 ctcattttat atacatctaa aaattcacct agatctgttc ttgcccctct aggcatgacc
      181 gcttcgattt ggaagcccat ttttcggtaa aggtgtacgg ctctttcatt acgaacttga
      241 acgtcaagct ccaatcgaaa aagtacaccc atttccttcg cccaactgat gatttcttct
      301 agcataagag tgcctagtcc cattccccaa tattcctgca gaatactgat accgacttct
      361 cccacgtgag acaaacgaaa ttgactgtct gccttgacag atgctgttcc aataatcgtc
      421 ccttcataga ttgcaagcag caataaatta ttattgcttt cgtatatata atccaactct
      481 tcttctaagg tttctggtgg taacaggagt tctgcttcat ccaaaactaa aaattctgtc
      541 tccttattaa cttgtcccat catcaaaaga atgtcttttg catctcctgg cactgcttcc
      601 cgcaagatga tcccaacttc ttcactcatg attttcagcc catttttcta gaattcgctg
      661 tttgaaggtt tcactttgct ccccgtatga gattagtttc acataccgct tttctaaatc
      721 tgtatcactt ttttctaaaa tcagttccaa ataatcctcc ggcagtgctt cttctaatag
      781 atttccccat tcaacaacgg aaagcccatc accttcaaaa tattcatcta accctaaatc
      841 tgctccgcta gctgctaccc gataaatatc catatgatac aacggaatcc gaccttgact
      901 atattctcta ataatcgtgt atgtcggact tttgatcatt tgcgttatcc ctaatccttg
      961 ggcaattcct ttcgtcaacg ttgttttacc cgcacctaga tcaccagtca atatcagatt
     1021 gtcacctggc attgcggaat gaccaattgc ctctgcgaat tgttctgtca ttgttaatcc
     1081 tgatagttcg atcatttttc atcttctccc gctcttcttt ctttttgcca gtatatcgcg
     1141 taatgaagtt tttttaaaga aaaaatgaac tagtcaagaa cagcagctgc tgtttttggc
     1201 tagttcgcgt gtatttgtta atcaatcaat gtttgtgctg ctgtgataat cgataattta
     1261 tatacatctt cttcatttgc tccacgagac aaatcagaaa ccggtttgtt caagccttgt
     1321 agaattggtc cgattgcttc aaagttaccg aaacgctgag caattttata gccgatattt
     1381 cctgactgca attctggaaa tacaaagact gttgcttgac cagctacttc tgaatctggc
     1441 gctttcagtt gtgcaactga aggaacataa gaagcatcaa attgcaattc gccgtcgatc
     1501 atgtattgtg gcgctagttt tttagcgatt ttagttgctt caactacttt gtctacttct
     1561 ggcgatttcg ctgagccttt tgtcgagaag ctcatcattg ctacttttgg ttcgatatcg
     1621 aacaattctg ctgtttttgc agaatctacg gcgatttcag ccaattcttg cgcagttgga
     1681 ttcacgttga tcgcacaatc cgaaaagaca tatttttctt gatctcttcc acgtaccatg
     1741 atcatcgctc cgcttgtccg actgatgcca ggctttgttt tgatgatctg aagtgctggg
     1801 cgaacagtgt cgccagtcga atggatcgct ccagagacca ttccgtctgc aatccccata
     1861 tacgtaagca tcgtaccaaa gtaattcaca tcttttaaaa tcttttctgc ttgttctttg
     1921 gatgcctttc catttctacg ttcaacaaaa gtatccacca tttcatccca cttatcgtaa
     1981 ttatcagggc tgatgatcgt aaagctagat gttttgattc ctctggcatg cgctgcattt
     2041 cttatttctt cttcgttccc tactaaaatg ggttccacaa gttcttctgc tttcaaccgg
     2101 gcagctgcgc ctaaaatacg agcatcagta gcttccggga aaacgatttt gatcccacgg
     2161 cgaacaattt taaatcttaa actatcaaac aattccacaa gatgacctcc agacgttttt
     2221 atatgtatta tcatacacta ttccagaaaa aaagaacagt ttattttatc tgtatcagtc
     2281 aaaaatgctg gaaaaattaa gtttttcaca tacaaagcga actgcactat aacaggaaag
     2341 cgctgtttta ttacactgtt tcaggcagtt gccaatcaat cggttcttct cctaacgcaa
     2401 ggagcgcatc attcgttttc gagaagggtt tggaaccaaa aaagccacga cttgcagata
     2461 acggactggg atgtggagat gttatgatga tatggcgact cttatcgatc attttgattt
     2521 tttcttgtgc cggttttccc cacaagataa acacgattgg tttttcccgt tcatttaatt
     2581 tttcaatgat cgtgtctgtt aatctttccc aacctttccc gcgatgagaa tacgcttgcc
     2641 cttcccgaac ggtaagaacg gtgttgagta aaaggactcc ctgtttagcc catgacacta
     2701 gatttccatg ttgaacaggt gcgattccaa gatcatcata aagctcttta tagatatttc
     2761 ttaacgatgg cgggatcttt acgccaggtt gcacagaaaa agataagcca tgagcttgat
     2821 tttctccatg ataaggatct tgtcctaaaa tcaccacttt aacttcttca taaggagtca
     2881 attccaatgc ttcatagata tgatacatat ctggatggat cttttgggac gcatattctt
     2941 tttttaaaaa ttctcttaga tgcagataat aatcttttga aaattctgag gacaaaactt
     3001 cttgccaact gttatgaatg atttctttca aaaactccac ctcctaataa aaagaataac
     3061 tcacattttc ttcaatcaca agggacaatc ttcaccctca gcacttgatt aatatggtaa
     3121 actaggaaca agaagcagaa aggaacgata atcattatga tcaaattaat tgcatcagat
     3181 atggacggca cattattaga tgcccatatg agtgtttctc ctgaaaatgc cgaggcgata
     3241 cgttttgcaa atgatgccgg tgttgagttc atggtcgcta ctggtcgaaa ctatcaagaa
     3301 gctcgcgcag ctttagatga ggtaggaatc gattgtgcga tgatcacttt gaacggggca
     3361 caggtatttg ataaagaagg aaattcattg tttaccgtgt cgattccaaa taatcaagca
     3421 atctctgttt tagatattct agatgctaac ggtatttact atgaagtagc aaccaacgat
     3481 ggtctttatt ctgaaaatca agcgaaacga attgaaagtt ttgcttccat ggtggctacc
     3541 catttacctc accttacgta taaaatggcg attgccatgg cctcagctaa gctggagtta
     3601 ttgcacatca catacgtgga cagtattcgt gctattttgg aagaaaagaa tctggaagtt
     3661 ttaaaaatta tttgttttca tacagaaggg ccagctgtat taggtccagt tggaaagcag
     3721 atttctcttt taggcgatct agccgttact tcctctggtc aaaacaatat cgaagtgaac
     3781 cataaaaacg ctcaaaaagg aattgctgtt gcccatgtcg ctcacgagcg tgggatttct
     3841 ttagaagaag tcatgacgat tggcgataac ttcaatgatg tcagcatgct gcaagtagca
     3901 ggagtcagtt tcgcaatggg aaatgcagaa atcgaagtaa aagattatgc caaatataca
     3961 acggacacaa accttgaatc tggcgtagga aaagccatat tgcgcgcgat tgcagaagac
     4021 ttgtagtttt atctagaaaa gagggatata gatgtcagaa tttttcattc aggaaaagca
     4081 gcttggacgt gcaactcgga caattgtcaa aaacgaaaaa ggacattccc tgtttttgat
     4141 ggttggtcgc tggggaacaa agggcgatgc attgtctctt tatgccatga atggcgatct
     4201 cgttgctcac atcaagcaga taagcctgac attcggtacg cgttttgagc tgtataaaga
     4261 gttcgaaaag gtgggaacca tgcagaaaat attcaattgg cccggtgact tttattatat
     4321 tcgtagactt cactggacag tccaaggtga tatctataat catcaatatc aaatccatca
     4381 tttcaatcag caaatcatga aaatggataa agcaacactt ttttcaggag attactatgt
     4441 attggatgtc tcaaatgatg aagatgctcc agtttgtatt tgtattgctg cggttttaga
     4501 ttactggctc taccgtaaag acaaaagtcg cgataagcct gtttcttacc gacttaactt
     4561 aagttaaaaa gatgtacctg aaatgcggaa atacgcattt caggtacatc tttttagata
     4621 ttcttttgca tatgatcata aagatgacca ctgatcatcc gctgaccgac aactttgaag
     4681 tcatggcgtt cgtataattt tttagccttt ggattttttt catccacact tagaccgatg
     4741 acggagcgat tagctttctt cgccaattta ggtaatgctt ctaataatcg cgaaccgatg
     4801 ccttgtcctc taaaatcttc tgatacactg atagagtcca aataccattc gttgggaaac
     4861 gtttctttat caatgaacaa acgtgtagac gcatctagct gatatttctc taagataggt
     4921 accagtggct gatcgatgat tgattcttct tcatctgtat aaccgtaagc aactcctgca
     4981 actttaccgt ctatttcttc tacgatacct cgtgaataac tgtaacgata atttggatca
     5041 gtgattgctt cttctaaaat ctctaaagtc ttttgttccc ctgtctgctt caaaaatggc
     5101 agttccatat cttttagaat cactaagatc aatggggcga tttctggagc atcttctttt
     5161 ctcgcaaaac gaatcatagt ttcctctcct tttctccatt ctaacatgca gaaaaagaaa
     5221 tcgcaaaatc gttattttaa aaaatcttca atcttttcag taatgaaata accatgagta
     5281 acgattcttg ccgcttcagt tcggcaataa gggcagacga ctggcatttc tttatcatta
     5341 tgttcgacta cattattgac ttcttcgtat gcaaattttt ttccgcaatt agggcatttc
     5401 attttttcca tgttatctca acttcctttc atctttactc tcttagctta ctcttatatt
     5461 gtaaaaaaga gaagcaatat ccttcttcta tgcagatatc catacctgac ggtcctgttc
     5521 tccatctatt aatcggactt tataataagg acgttcatct ttgactagaa gtgtccactc
     5581 tttcactttt gcttttttcc cgtagtagct gactgcttcc ttcatcgctg cttgcggttg
     5641 tttgaactct tccgtttttt ctatatcaaa aagatgttct gcggcctttt ccgtgtcatt
     5701 tcgtacggtt gttttcccag tggatggatt gactgctatg acttgtcctt cacaaaaaaa
     5761 ctggtattca tatgttgcat tcgtaggatt ctccaaatct ttttcaactg caaaatgaat
     5821 agcagatatg ttatgttctt ttgtttcgtt ttttacgatt gctgctgctt ttttcaatga
     5881 gatccgatat tcctggtcac taatatcatc catgtgaaaa gcggcattga gcagtcccgt
     5941 cataccggta atccctatta cgatgagggc agcaacacca aacatcattt ttttcattct
     6001 tcattctcct gtttcttttt tttaaagaat atcaagaaat gaacgaatga aaaatcgaat
     6061 gaaacgaaaa aaaagtgaca attttgttat ctttattcgt gcagttctaa tggcaatccg
     6121 tctggatcaa agaaaaacgt catttttttt cctgtaaagg tatcttctct aatcggttct
     6181 gattcgatcc ctaaggactg caattctgca attgtttctt caatatcgtc tactttgaaa
     6241 gcaagatgac gcaagccaca agcttctgga tagtttggtc gttgtggcgc attagcaatt
     6301 ccaaaaatct ccaattcagc acttcctagt ttcagatcta atttatagtc atctcgttct
     6361 ttgcggtaat tttctctgat tacttcaaat cctaatttat taacataaaa atcacgtgat
     6421 ttctgataat ctgaaacaat gatggctata tgatggatcg ttgtaaggtt catatgagca
     6481 ctcctattct tcgtttaagc tcggatctat attaatctcc agttccattg taaatgtgtc
     6541 agattgaaaa aaatagctgt ttttcctttt tataagaaaa aaggcaaaaa aaacagaaag
     6601 taggtcctcc aagacactct tcctgttccc aatataataa atgaaagtaa gaaattatat
     6661 tttcaagaaa acaaacagaa aattatcata acacaaatta tttataattg aaagactagt
     6721 tttttttcaa gaaaagcata caatagattg tgttagtaaa tacagaacac actacattta
     6781 gtgtcttaag gagtcctcat acatgaagtc atattttcag tttttagctc atcagctaaa
     6841 aggctggccg caacaaaatt attatctttt ctttttcagt cttggctgcc aggtcatgac
     6901 tcttgtcaat agtcccatca cttggctgtc cgttattaca tttatcggta ccacgcttgg
     6961 tgtattgtgt attttatcca tcaatgcggc aaaatcagtc aacggtattt taggtatttt
     7021 gtctgcgatt tgttttatta tcgttgggtt ttcagcaaag aactatttaa gtatcggtga
     7081 acagattgct tatatgatca cattggatat ccccgtattg ttaagtaaag aatggaatca
     7141 taatatggct tcaaaaatcc gaaaattcac tggaaaaagc tggtttatcg caatcatcag
     7201 tactttcctt gtctatcttg tttcaggatt cctgattggt cgttatacag atgatcctcg
     7261 gccgtgggta gatgccattg ctttttctat tagcttgact gctggtatca tcagtttctt
     7321 gcgttacaac aaccaatatt actggtggtt agcttcaggg cttgctcaaa tggtcctttg
     7381 gtatatttca tttacgcatg gatctgcaag tttggcgatg ttcgtgaaca gctctgttta
     7441 cttgctgaat gatgtccttg catttaccgt ttcaccttgg tataaccaaa aagaacgtga
     7501 acgtatgcaa aaaatggaag aagaatacta caacgcacaa caacacgcat aaaaaaactg
     7561 agcgtgggac aaaagtaaag aacacttttg tctcacgctc taaacacgta taaacggcgg
     7621 aatcagaagc aactccttcg gaaataagct gaaattcaca aaaatttgaa aagcaatttt
     7681 cgtgaatttc ttcttatttc ttggagttaa acgcttctgt cccagcctct attttttata
     7741 gtaattcctt cagatacttt cttgcctcat cattgaaacg cagtgattct tctaacttct
     7801 gataatattc caacgcttgt ttctgagctt cagtaaaacg ataatgtttt cctttccatt
     7861 tgtcgattgt ctcttgcaca tttccagatt ggatatcttt cactactagt tgccattttt
     7921 cttttgagac tgtacggtct ttcttatcag cagattgtat agcttctcgt tctttacgca
     7981 atacttttaa aaaaccattt ctgactatcc gaggaatgct gtcgaagaac ccttctgtaa
     8041 aaccatagcg acaatattgc ttccaaataa atagatagat ttttggttgg atgaatcgtt
     8101 ggtttttcgc aaaatattcc atctgagcag tgacttctgg tgttcgttga cgttttgtct
     8161 ttctcaacac ggaaataaat gtttcaaaat aagcattagg atgtactggc acttcttcat
     8221 atacgatgga ttgccaatct ttacgtaaaa atgcgcgata gttgatcaag aattcttcta
     8281 catcctttac taattcttct gcaaagtcgt caaatagttc tttttttatc tgtcgctttt
     8341 ttgactgccc attgatgtgt tcacttattt tcttgatttc attttccaat gcttgataat
     8401 ccgctatgta aatcggcatt tctacttggc gaaattctgc gagccgctga gctaatttta
     8461 tatccggtaa atatggctgt ttcatccgac gattttggta caagacaaaa cagtattttt
     8521 gccatagtcc ttcaggaaaa tcgatattct tctgcttaag ccataataat tcgtctaagg
     8581 ccagatcaac ttttgtcatt ccctgatgga tcgaagctgc cactgtcgga gagacatgca
     8641 ggtgatcggc aaagtggttg atgcctagtt ttaggatctt tccggttttt ggcgatttaa
     8701 cgatgtattg cacttttaat tctctaccac actcacaata caatttttgc tggcttcgtt
     8761 ttgttcgaaa aaacggattg atcttgaagt cagaaaacac ccaatctgtt gcagctaaat
     8821 aatgttcttc cataaaacga gagctgatta gatatcggcg gtgcttatcg actagttccc
     8881 gctgtttttt cgttaatttt gcatagatct cccaacgttg actgcttgtg tattgtttag
     8941 aaattttgat gggatagctt ttgctgattt cttttggaaa ccattcttca aagacggatt
     9001 gccattcttc cacggctgtt tctttccttt ctaattacaa aaagctggct actaacataa
     9061 cgttagcaac cagccagtgt ttcttgctaa attagtgagt tgttccggca tttccggaaa
     9121 tatcttcttt attttctact gctgcttcaa ctgcataagt caaagaagaa gcaatgtctt
     9181 ctaaagacat agaaggcgta cccactggac gatcgatcac ttgatcgggt gagaatggca
     9241 cgtggatgaa cccacctcga attgccggga attgtgtttc gatcaaatga agcaaacgat
     9301 acatgatatc attgcagaca aatgttcctg ctgtataaga gatgtacgct ggtagaccat
     9361 gatcttgtac attttttgtc attgctttga ttggcaatga ggtaaagtaa gcggctggac
     9421 cgttttcttc taaagccgta tcgaatggct gatttccttc attatcaggg attcttgctt
     9481 ctgctagatt gattgcgaca cgttcgaacg agactgctga ccgcccacct gcttgtccga
     9541 cacagatcac gacatctggt tggtgctgtt ggatggcttc tttgactact tgactgcttt
     9601 tttcgaatac ggtcggtact tccactttga tgatttcagc accgctgatt tcatctggca
     9661 ttttttttac tgcttcatag gctggattca ctttgtcccc accaaatgga tcaaaacctg
     9721 ttactagtac tttcatctgt tgttcctccg tccgattgat ttaacatatg gttatctttt
     9781 atcataccat tttccttgaa cagcgtaaat aaaggaaaca atagtttaaa taacataaca
     9841 gatcaaactt aatatattct tataaaaaaa tagtgttcca ctacaatgtt agaagaggtt
     9901 gcgacaatta ttctgtcaca acccctctct tctattattt aaatagaatc atataagcaa
     9961 tttggaaaac tagcataaat aacgcaataa acaactggtt tttgatcact ccgtatttgt
    10021 ctttcatctc cagcatcgct acaggcacga tattgaagtt agcagccatc ggtgtcagca
    10081 gggtcccaca aaaaccgcaa gtcaatgcaa ccattcctat taacgctgga ttcgctccat
    10141 gactaaatac gaatggtgca ccgatcccaa ccgtcatcac ggtgatggcc gcaaatgcat
    10201 tccccatgat taccgtaaat atcaccatgc caagagcgta aatgatgatg ccgatgttca
    10261 catttccttc tggaacgatc gtacctactg cattggaaat cactgtccca actccagcgc
    10321 ttgtaaatac cgcacctaga gaagctaaaa gcattggcaa catgcttaac ggccctactg
    10381 tgcccagcat atctgctgca tcatctaaaa atgtacttgg tcgattatct cgagaaaaga
    10441 acatcaagat tagcatggct gcaaaaacac cgacgcccac gccgactaaa gcacctaaat
    10501 tggtaaatag agcaaacaag atcgcaaaga caccgatact caaagctgga ataaaaattt
    10561 tcattcctag tttgtctgac attttttcca tatattcttt tgatggaagc cggctttctc
    10621 ctttgctgac tttcttagca attgctggta tggtcatcgc aaagatcaag ccaccgttca
    10681 cgattgccgg aagccatctt cctcctgcaa taactacccc taagaccgtc cagaaaaagg
    10741 ctgacccata acgatgagga ttggttttat ctgttaaatt tcttactcca gtgtaaatca
    10801 atacgagtcc catcacgata taaaggatct ccagtaattt atcgcctaat aaaatatcag
    10861 gatttgtaaa aaagtccatc ttattgcccc tttcctggat aatatttctt cgctaatttc
    10921 ttttctttaa gatagaagta gacacttgcc acaactaaag ccgtgatagc cactggaact
    10981 tccactaaag caagctgtcc caatgttacg tcatatccta gatctttcag tgttgattgg
    11041 actagcaacg cccctgctcc tccgatgaat agtacttggc caaagaacca gcagacattt
    11101 tccatagctg aagccatacc ttttagttct tcttcatgct taggatggac ttctaaccct
    11161 tttgattcga tggtccccaa tgccatcggt aaaatgattg gacgaacaaa tccagcaact
    11221 ccaccaaaac tcacgttaaa agccgcaaag atcattcgaa agatcccata aatatctaca
    11281 atcataccag cagatacttt cttgaatcgc ttgatcaagg aagccgctga ttctttcaga
    11341 ccatttcttt caagcgttcc tgttgccaac ataatgatga taaaaatcgc catcccgcga
    11401 ttgtttacaa aacttgtacc taaaagttct aacattcctt gaatgccaag accgccagtc
    11461 aaagctgtaa caactaaagc aaccattacg atcaatatcg agtctaattt aaatgcaaac
    11521 ccgagaataa caatcaagac gcctaaaagg ctaagataat tttccatata ttttcctcca
    11581 taaaatgaca atattttttt aattataaag caaaacacat aaaaaatata gttgttttct
    11641 cacgaaatag acactatttt taaatatttt tcgttatt
//
LOCUS       DARBET010000078        11667 bp    DNA     linear   BCT 07-NOV-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4027
            SAMEA114569841-rid20112183.denovo.080, whole genome shotgun
            sequence.
ACCESSION   DARBET010000078 DARBET010000000
VERSION     DARBET010000078.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA114569841
            Sequence Read Archive: ERR12203666
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 11667)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11667)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001972618.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001972618.1
            Genome Coverage       :: 132x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/07/2023 13:18:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,041
            CDSs (total)                      :: 2,975
            Genes (coding)                    :: 2,908
            CDSs (with protein)               :: 2,908
            Genes (RNA)                       :: 66
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 67
            CDSs (without protein)            :: 67
            Pseudo Genes (ambiguous residues) :: 0 of 67
            Pseudo Genes (frameshifted)       :: 26 of 67
            Pseudo Genes (incomplete)         :: 28 of 67
            Pseudo Genes (internal stop)      :: 20 of 67
            Pseudo Genes (multiple problems)  :: 6 of 67
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11667
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA114569841-rid20112183.denovo.080"
                     /isolate="VRE4027"
                     /db_xref="taxon:1352"
                     /country="Denmark"
     gene            complement(37..216)
                     /locus_tag="SCJ86_002330"
     CDS             complement(37..216)
                     /locus_tag="SCJ86_002330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295613.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEG1169852.1"
                     /translation="MPIAIGNKRLPVTLDEKRQKELQQLKQKYGKSESRIMCIALDLL
                     IAQEKAGFEVPALKK"
     gene            complement(597..1304)
                     /locus_tag="SCJ86_002331"
     CDS             complement(597..1304)
                     /locus_tag="SCJ86_002331"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEG1169853.1"
                     /translation="MNKVVYQLTTEDVSQFLTNKEHLNEVIRRIQKDFSCMEEMKYFI
                     QRVEEQLYMESETSQTKVTVSHNRLDVYKRRPDTLKKGAELLRLETEKYIMNVYLERS
                     ELVENFVSYTICRKHYYSESEYYSIFESMELMEEEMEELNSKDATVDDYYLVFTRFLT
                     CGTESIVLMGMDYELTVPEAFLREYVEEVDNPGDDWYTTLTDFLDTYTYDDTEEIQNE
                     LDRKRMPYQRFERKKGQ"
     gene            complement(1297..1734)
                     /locus_tag="SCJ86_002332"
     CDS             complement(1297..1734)
                     /locus_tag="SCJ86_002332"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016922517.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEG1169854.1"
                     /translation="MAQNYYDEFVKLPLDKMAQKMEDMTFLYHETRVPKKHYKEKLSV
                     AVEEMIESGVEMNLIATYYRTLEELKKQNGKWFFQALLCLEVGVKPSTIKPSEYQALE
                     LTYAKFAQIKKAKAMASDWLDTFENIEKNGAFYTIESEEKDNE"
     gene            complement(1749..2000)
                     /locus_tag="SCJ86_002333"
     CDS             complement(1749..2000)
                     /locus_tag="SCJ86_002333"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314430.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEG1169855.1"
                     /translation="MKTQHKLLTVKEVVQILVDKEVISEKVAEALLIPRYWTIETPLV
                     EDAPTMLFEDLVKIAGYELHYQENMETYEIEYWLTKLEN"
     gene            complement(2000..2206)
                     /locus_tag="SCJ86_002334"
     CDS             complement(2000..2206)
                     /locus_tag="SCJ86_002334"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853414.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA helicase UvrA"
                     /protein_id="HEG1169856.1"
                     /translation="MRKKFDYWGVPFSELFPNYHAPHTVECDCGERAKCIKSYRLYQC
                     PTCGKKYTLSYGDYVLIDEKVKKR"
     gene            complement(2643..2903)
                     /locus_tag="SCJ86_002335"
     CDS             complement(2643..2903)
                     /locus_tag="SCJ86_002335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339774.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEG1169857.1"
                     /translation="MNKYVGLLDKIRVIKTQPLLVRFTLQTIHESINCVVADIEIIDK
                     LLIMDDGKYNIAVTGHFNKRNQLVIESMQIRNPDHFTRSMGI"
     gene            2978..3181
                     /locus_tag="SCJ86_002336"
     CDS             2978..3181
                     /locus_tag="SCJ86_002336"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002316074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative holin-like toxin"
                     /protein_id="HEG1169858.1"
                     /translation="MIKTVKFVTVAIRLKVRWCVCAYFQNLTERRSLLPVETALGLMI
                     SFGSLIATIIFGILAVVNNNQKK"
     gene            complement(3322..3807)
                     /gene="ssb"
                     /locus_tag="SCJ86_002337"
     CDS             complement(3322..3807)
                     /gene="ssb"
                     /locus_tag="SCJ86_002337"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000609607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded DNA-binding protein"
                     /protein_id="HEG1169859.1"
                     /translation="MINNVTLVGRLTKDPDLRYTASGTAVATFTLAVNRNFTNQNGNR
                     EADFINCVIWRKPAETMATLAKKGSLIGVVGRIQTRTYDNQQGQRVYVTEVVADNFQL
                     LESKAATESRAHADQSSSSPSPTTFEQRDVATPNNNGLNASQNPFGGQSIDISDDDLP
                     F"
     gene            complement(3810..4709)
                     /locus_tag="SCJ86_002338"
     CDS             complement(3810..4709)
                     /locus_tag="SCJ86_002338"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303312.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyl transferase AbiEii/AbiGii toxin
                     family protein"
                     /protein_id="HEG1169860.1"
                     /translation="MTPTQFAAKTRNLAKSTGLNAQLVQRHFMMDRLIEQISESKYKN
                     DFILKGGFLLGSKYGIDRRTTIDIDTTFRNSKLTEEKLKSIFNDLTRKPTKEGIVFSI
                     QGMKETREADFYPGFQIKLVANLEKVRVPFKMDVTTGDSIYPEVDTHYHQLMFEDKKV
                     EIPAYPTEQIIAEKLSATFSFGTDNSRAKDYYDLFTIPKLENIDTKELYKSVRNTFTK
                     RGDTKPLSFYYEREMDKIRGNDYLEKQWEKYRSVNTFANSISFTDTVNEIDHLMKSVI
                     KIENQERKRQFKRSQQMNQEMER"
     gene            complement(4722..5315)
                     /locus_tag="SCJ86_002339"
     CDS             complement(4722..5315)
                     /locus_tag="SCJ86_002339"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010737159.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IV toxin-antitoxin system AbiEi family
                     antitoxin domain-containing protein"
                     /protein_id="HEG1169861.1"
                     /translation="MQDKLKELLAIRDGNLSMKEARKFGIAATTVQRLVNQGKLIKVD
                     RGYYVEDGHGIDDLFWLQQRFSRGIFSHETTLDIYQLSTNMPKFIHLTFPHGYNVDRS
                     ITKEQQLQFHFVKKEVYELGKVEGTSFQGNPITMYDKERTLCDIWNPRYEFEYEMKLE
                     ALKEYMEDPLRDPSKLRDYMTKLHVEKEMKYHMQSLY"
     gene            complement(5391..5540)
                     /locus_tag="SCJ86_002340"
     CDS             complement(5391..5540)
                     /locus_tag="SCJ86_002340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344867.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEG1169862.1"
                     /translation="MRNTTHKQQEIENQKAKIRLEQEKLRANKRRLQQLKNQQRIQKE
                     IELDR"
     gene            complement(5556..7760)
                     /locus_tag="SCJ86_002341"
     CDS             complement(5556..7760)
                     /locus_tag="SCJ86_002341"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002369673.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase 3"
                     /protein_id="HEG1169863.1"
                     /translation="MKTVILAEKPSQAKAYADSFSKATRKDGYFEIQDRLFSGETVIT
                     YGFGHLVELDSPDMYDENWKQWSLEHLPIFPTHYHYHVPKDKKKQFKVVKQQLQSADT
                     IIIATDSDREGELIAWTIIQQAGADHGKIFKRLWINSLEKEAIYQGFQQLRDAEETYP
                     KFEEAQARQIADWLIGMNGSPLYSLLLQQKGIPGSFSLGRVQTPTLYMIYQLQEKIRN
                     FQKEPYFEGKAQVIAQNGAFDAKLDPNETQATQEAFEDYLKEKGVQLGKQPGTIHQVE
                     TEKKSAASPRLFSLSSLQSKMNQLMKASAKDTLEAMQGLYEGKYLSYPRTDTPYITEG
                     EYAYLLDHLDEYKHFLKAEAIPTPIHTPNSRYVNNKKVQEHYAIIPTKTVMTAAAFEQ
                     LSPLQQAIYEQVLKTTVAMFAEKYTYEETTILTQVQQLQLKTVGKVPVDLGWKKLFGK
                     ESDRKEKEEEPLLPKVTKGEAVTVDLQVLEKETKPPQPYTEGTLITAMKTAGKTVDSE
                     EAQSILKEVEGIGTEATRANIIETLKQKEYIKVEKNKLVVTNKGILLCQAVEKEPLLT
                     SAEMTAKWESYLLKIGEQKGTQATFLANIQKFVSHLLEVVPGQIQSTDFGSTLQEVKA
                     ASEKQEATRHLGVCPKCREQEVLLYQNVAACTSEACDFKLWTTIAKKKLTATQLKEII
                     QNGRTSQSVRGLKGQKGSFEATIVLKEDFTTGFEFSEKKKTNYKKRTRRTTK"
     gene            complement(7771..9231)
                     /locus_tag="SCJ86_002342"
     CDS             complement(7771..9231)
                     /locus_tag="SCJ86_002342"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014401506.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="toprim domain-containing protein"
                     /protein_id="HEG1169864.1"
                     /translation="MTEKKMSLIDRCKQIDIVDFARNNGMAVVNKGRDYRLEDHDSFV
                     FDRRKQRFYWNSQNISGDIIELAKLFFIDKEIQDPKQQFKAATDFILKNEDKTERVEN
                     LHFETEKYKDHPVDYQPLTEKGRNYLKEERKLPEWLIDYAEKEGLIAELKPKHERQNF
                     LVRDDRLDHAVAFLWKDPQTNETVGASYQGTFIDYERFGERGTYKHIDKNSTANHGFN
                     LKIGDPKQLKFFESSIDLLSYAALNRDQLNDTWLVSMEGLKHHVISHYFGEAVSELRK
                     KQAFPQSIEICVDNDRAGHIFYEKEQLMGAVDPFTNQKVRCERGIANDWQVPKEYKVI
                     YEEVAKEEKITPEAIMAIHKTENNLQLTNQLVSAHKVNASFGQQLSVNDSIEAINLKD
                     ICREVAKELKACERVDGTYDFDRFYQEKGDINAQILFSYKAEQYYKGYKNHEHEFVPE
                     VKKDWNDQLKHEIYQQEIRKQKRAMLFQQGRQQERE"
     gene            complement(9292..11664)
                     /locus_tag="SCJ86_002343"
     CDS             complement(9292..11664)
                     /locus_tag="SCJ86_002343"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347694.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEG1169865.1"
                     /translation="MAEEKEPLDNSRLGKSKRKLVRLQNELNEQIEKMFEHQRKTNGQ
                     PMNDKRNGHSWFRQQERLENKVHSLREEIKQQEKQVEKLERQEELKEMGYNKYGGLDM
                     TIENIPRIKEEIERFEKGESTFSAATIRKYQRKLETLEQLKERSEKGKENLLPEVQAI
                     IDSGRVTQWKKNPTIYFLKDYRKVALELNENGGFEESKKYQATSEEEKAAVRELLEEA
                     APTDSLKEKENDHFYRVEFNENDPEKGLKSYGGEIVTLELIDQLKTLDNQYAAEAGYY
                     KFYFEEVKNGEVVDQIRMDLGDGVNSNQPIYLELAKLCEQVPEKEQKQTVKNEPVSKE
                     QMQSKETKTKQKGYQKRTAEEIKQEVKSLSQQALDAVKKHTHSPEDVKELLNFMSRFP
                     ERSFRNQLLIEEQFPGATACLGRAALKKEGIYIKKGEQGNKIFVRKTVKGFYEKGRGF
                     VRETEATPEDKKRIESGQIEVIKKPYYTIEKVFDITQTQLKPEDYPKIFPNRVFDFQL
                     DKKGQSELQAGIQAVANNIGIQIRDMTESEVYQRELGQARGAYVRNEFTGEEEIVMNT
                     RNTPTQHLATSIHELAHARMHKFSELDTATKELQAEMTSYIVCKHFGMDTSEKAIPYI
                     AAWTKNGQTLDDKEAAERGKIMNDVSRVANEFIQTISNEINQYREREPEIQPAEVKQE
                     HKNTVETAEQKNIVPVGSLWIDKKDGKVMEEDTGRTLHLKDAAFIEDPQHNQVILKGK
                     SYGKEIQTALPSEEFALGNSFRFSLETVSEVAKRKNEIYYEQQLSQKERV"
BASE COUNT         2892 a         2432 c         1957 g         4386 t
ORIGIN      
        1 tgctatcact tttagttttt aggataaaaa gtgacgtcac tttttgagtg ctggtacctc
       61 aaatcctgct ttttcttggg caatcaataa atctaacgca atacacataa tcctggattc
      121 acttttgccg tacttctgct ttagttgctg caattctttt tgtctctttt catccaacgt
      181 cacgggtaac cgtttatttc ctattgcaat tggcattagt actctctcct ttcatcgaaa
      241 taaccctatt aaaaatatgg ctgcaatcaa cttgaatatt agactcacaa gggttaacac
      301 aaagcgccaa aagatcaaac aaaataccag tattcctaag aatactacga agtttaaatg
      361 tagagctgca ccatgacgca taagtatctc gccaacaaac agtacgaaca atagaggaat
      421 gattttattc atatggatct ccttctttct cttaggttat cgggctttgc ccgcttattt
      481 cgcaccacct aagcggcggg ggcggttgga tcgattggtg cgaaataaga accattccct
      541 taatttcaag gttttttgtt cttatttcac acatcctcac ttttcggtat aaatggttat
      601 tgaccttttt tcctttcaaa tcgttgatat ggcattcgtt tacggtcaag ttcattttgg
      661 atttcttctg tatcgtcgta agtgtaggtg tctaaaaaat cagttaaagt cgtgtaccag
      721 tcgtcgcctg ggttatctac ttcttctaca tattccctca aaaatgcttc gggtacagtg
      781 agttcataat ccattcccat taacacgata gattccgtcc cacacgtcaa aaatctagta
      841 aatacaaggt aataatcgtc taccgtcgca tctttgctgt ttagctcttc catttcttct
      901 tccattaatt ccatgctctc aaaaattgaa taatattctg attctgagta atagtgtttc
      961 ctgcaaatcg tgtagctgac aaagttttcg accagctcac tcctttctag gtaaacattc
     1021 atgatatatt tttctgtttc tagtcgtaga agctccgctc ctttttttaa tgtgtcagga
     1081 cgacgtttat acacgtctaa acgattatgg ctgactgtaa ccttcgtttg actcgtttca
     1141 ctttccatat acaactgttc ttccactctt tgaataaagt acttcatttc ttccatacaa
     1201 ctaaagtctt tttgaatccg tctgattact tcattcaagt gttctttgtt tgttaaaaat
     1261 tgcgaaacat cttctgttgt tagctgataa accaccttat tcattatctt tctcctccga
     1321 ttcaatcgta taaaatgcac catttttttc gatattttca aaagtatcta gccagtcgct
     1381 agccattgct ttagctttct taatctgtgc gaattttgca taagtcagtt ctaaggcttg
     1441 atattcggaa ggtttgatag tgcttggttt gacacctact tccaagcata gtaaggcttg
     1501 gaaaaaccat tttccatttt gttttttcag ttcttccagt gttcgatagt acgtcgcaat
     1561 gagattcatt tcgacacctg attcaatcat ttcttccact gcgactgaca atttttcctt
     1621 gtaatgtttt ttgggtacac gtgtttcatg gtataggaac gtcatatcct ccattttttg
     1681 tgccatttta tccaaaggta atttgacaaa ttcgtcatag taattttgag ccatattttt
     1741 tctccttttt agttctctaa tttggttaac caatattcga tttcataagt ttccatgttt
     1801 tcttgataat gcagttcata tcctgcaatc ttcactaggt cttcaaatag catagtcggt
     1861 gcatcctcta ctaaaggggt ttctatcgtc cagtaccgcg gaataagtaa agcttctgct
     1921 actttttcag aaatgacttc tttgtccacc aaaatttgaa cgacttcttt tacagtcaat
     1981 aatttgtgtt gtgttttcat caccgtttct ttactttctc atcaatcaaa acataatcgc
     2041 cataagacaa ggtatatttt ttcccgcatg ttggacattg atacaaacga tagcttttga
     2101 tacatttcgc tctttctcca caatcgcatt cgacagtatg tggtgcatga tagtttggga
     2161 ataattcgct gaagggaaca ccccaataat caaatttctt tcgcatgatt gattctgcct
     2221 cctcttagga aaggttttat ttcctttcgt tagttctttc tttattgatt tgttggccag
     2281 aatgaatcaa cgaaaagcga caaggacaag ggcgcgcagc gtgccattta cccttgtcct
     2341 tgtcgctttt tggtgataaa aggcaacttg ttgccggtct ggcaaatcaa taaagagata
     2401 gcgaacagga acgaaccttt tggtgagagg aagttttttc tattttgtct tgctttgcaa
     2461 gacaccatat cttagtgaac gtgcaacgtt cacttccttt cttctcgaac ggcggtggcg
     2521 gttggagtga gagaaaggtt tttcaattga gaaggcactt gtgtcttcct aaaaaggtct
     2581 tttcctgttt ctatgtaaaa caaaaagagc tgcaatcctc atttaggact gcagctcttc
     2641 ttttatatgc ccatcgatcg cgtaaaatga tcggggtttc taatttgcat cgattcaatg
     2701 actaattgat tccgtttatt gaaatgacct gtgacggcaa tattgtattt tccatcatcc
     2761 ataattaaca atttatcgat aatttcaata tctgcaacta cacaattaat cgattcatga
     2821 attgtttgta aagtaaagcg aaccaataat ggttgtgttt ttattactcg gattttatca
     2881 agcaaaccaa catatttgtt catcacgatc acctcgctaa aattatagca aaaacagcta
     2941 aaattgagaa tttttattcg attttagata tgctataatg atcaagacag taaagtttgt
     3001 gacggtggct atccgattga aagtgaggtg gtgcgtatgt gcgtacttcc aaaatcttac
     3061 agaaaggaga agccttttgc ctgttgaaac ggcgctagga ttaatgatta gtttcggttc
     3121 tttgatcgca acaatcattt ttggaatctt agcagttgta aacaacaatc aaaaaaaata
     3181 accgtcagct ttagccggtc gacgattatt tttcaaaaat aatattaagc tggtcaccgt
     3241 cttaaacggt gctgttaggg gagttgttag cgcaactcct cttttcgtat ccattataac
     3301 aactttattg atcttttaca atcagaatgg taaatcatca tcactaatat cgattgattg
     3361 tccgccaaat ggattttgtg aagcgttcaa tccattgttg ttaggcgtag ctacatcccg
     3421 ttgttcaaag gtggtagggc ttggactgct ggacgattga tcagcgtgcg cacggctttc
     3481 tgtcgctgct ttactttcta atagttggaa attatcggcc acaacttctg tcacatacac
     3541 acgttgtcct tgttggttat cgtaggttcg ggtttgaatc cgtccaacaa cgccgatcaa
     3601 actgcccttt ttagctaatg tcgccatcgt ttctgctggt ttgcgccaaa tcacacagtt
     3661 aataaaatca gcttctcgat tgccgttttg attcgtgaaa tttcggttga cagctagggt
     3721 aaaagtcgct actgcagtac cactcgcggt ataacgtaaa tctggatctt tggttaagcg
     3781 tcctactaaa gttacattat tgatcatctt tatctctcca tttcttgatt catttgttgt
     3841 gatcttttaa actgccgttt tctttcttga ttttcaattt tgatcacact tttcattagg
     3901 tgatcaattt cgttgactgt atctgtaaat gaaatggaat tcgcaaacgt gttaactgag
     3961 cgatattttt cccactgctt ttccaaatag tcatttcctc tgatcttatc catttctctt
     4021 tcataataaa aggacaacgg ttttgtatcc ccacgttttg taaatgtatt tcgaacactt
     4081 ttgtataact ctttcgtatc gatattttct agtttcggaa tggtaaataa gtcgtagtaa
     4141 tcttttgctc tcgaattatc tgtgccaaag ctgaacgtag cacttagctt ttctgcaata
     4201 atttgttctg ttggataagc tggaatctcc acttttttat cttcaaacat taattggtga
     4261 tagtgtgtat ctacttcagg ataaatagaa tctcctgttg tcacgtccat tttaaacggt
     4321 acacgtactt tctctaaatt agccaccaat ttaatttgaa atccaggata aaaatcggct
     4381 tctcttgttt ccttcattcc ttgaatagag aaaacaatgc cttcttttgt gggttttcta
     4441 gttaaatcat taaaaatgct cttcaatttt tcttcagtta atttgctatt tctaaatgtc
     4501 gtatcgatat caattgttgt ccttctatcg attccatatt ttgatcctaa taaaaagccg
     4561 cctttcaaaa tgaagtcatt tttgtacttt gattcagaaa tttgttcaat tagacgatcc
     4621 atcataaaat gccgttgcac taactgtgca tttaatcctg tactctttgc taaatttctt
     4681 gtttttgctg caaattgtgt cggtgtcatc tttttcctcc atcaatataa gctttgcatg
     4741 tggtatttca tttctttttc tacatgtagt tttgtcatgt aatctcttaa tttacttgga
     4801 tctctcaatg ggtcttccat gtactctttt aatgcttcta atttcatttc atattcaaat
     4861 tcatatctcg gattccaaat atcacaaagt gtacgctctt tatcatacat agtaatagga
     4921 tttccttgaa aggaagttcc ctctactttt cctagttcat aaacttcttt cttcacgaaa
     4981 tgaaattgta actgctgctc ttttgtaatt gaacgatcaa cgttatagcc atggggaaaa
     5041 gttaagtgaa tgaatttcgg catattcgta gaaagctggt aaatatctaa agtcgtttca
     5101 tgagagaaga ttcccctgga aaatcgttgc tgaagccaaa ataaatcatc tattccatga
     5161 ccatcctcta cataataacc acgatcaact ttgatcaatt ttccttgatt caccaatctt
     5221 tgtacagtgg ttgctgcgat tccaaatttc ctggcttcct tcatagataa atttccatcg
     5281 cgaatagcca acaattcttt cagtttatct tgcatttact tcccctccct tcgttacaaa
     5341 tacactacat caaatatatc atagtagttt atttgtaaca agattaacgt ctatcgatct
     5401 aattcgattt ctttttgaat gcgctgctgg ttctttaatt gttgcagtct tcttttattt
     5461 gcacgtaatt tctcttgttc taaacgaatt tttgcttttt gattttcgat ttcttgttgt
     5521 ttgtgtgttg tatttcgcat aggataaccc cttcattatt ttgtagtgcg gcgtgttctt
     5581 ttcttatagt tcgttttttt cttttcagaa aattcaaagc ctgttgtaaa gtcttctttt
     5641 aaaacgatcg ttgcttcaaa cgaacctttc tgacctttta gtcctctgac tgattggctt
     5701 gttcggccgt tttgaatgat ctctttcagt tgtgtggctg tgagtttctt tttggcaatc
     5761 gttgtccata atttaaaatc acaggcttca gacgtacatg ccgccacatt ttgataaagc
     5821 aagacttctt gttcccgaca ttttggacaa acacctaaat gacgcgttgc ttcctgtttt
     5881 tcactggctg ctttcacctc ttgtaacgtt gatccaaaat ctgttgactg gatttgtccc
     5941 ggtacgactt ccaacaagtg agagacaaat ttttggatat tagctaagaa agtagcttgt
     6001 gttcctttct gctcaccaat ctttaataag taactttccc atttggcggt catttcggca
     6061 cttgttagca gcggctcttt ttccacggct tggcaaagta aaatcccttt atttgtcacc
     6121 acaagcttat ttttttctac cttgatatat tccttttgtt tcaaagtttc aataatattt
     6181 gctcgtgttg cttctgtacc gattccttct acttctttca atatggattg tgcttcttcg
     6241 ctatccacgg tttttccagc tgttttcatc gctgtgatca acgtaccttc ggtataaggt
     6301 tgtggtggct tcgtttcttt ttcaaggact tgtagatcta ccgtcaccgc ttctcctttt
     6361 gtcactttag gaagtagcgg ttcttcttcc ttttcttttc tgtcgctctc tttgccaaac
     6421 agctttttcc aacctagatc tactggtact tttccgactg tctttagctg aagctgctga
     6481 acttgcgtca agatcgttgt ttcttcatag gtatactttt ccgcaaacat ggccaccgtt
     6541 gtttttaaca cttgttcata aatagcctgc tgcagtggac ttaattgttc aaaagccgct
     6601 gccgtcatga ccgtctttgt aggaatgatc gcgtaatgtt cctggacttt cttattattc
     6661 acatatcgac tattcggtgt atgaatagga gtaggaatgg cttcggcctt taaaaagtgt
     6721 ttgtattcgt ctaagtgatc caacagatac gcatactccc cttctgtaat ataaggggta
     6781 tctgttcgtg ggtagcttag atatttccct tcatacaacc cttgcatggc ttccaaagta
     6841 tctttggcac tagctttcat gagctgattc attttagatt gaaggctgga tagagaaaaa
     6901 agacgtggac ttgccgcact ttttttctcg gtttcaactt ggtggatcgt acctggctgt
     6961 tttcccaatt gaaccccctt ttcttttaaa taatcttcaa atgcttcttg cgtcgcttgg
     7021 gtttcattcg gatcaagctt cgcatcaaat gcgccatttt gagcgattac ttgggctttt
     7081 ccttcaaaat atggttcttt ttggaagttt ctaatctttt cctgtaattg ataaatcatg
     7141 taaagcgttg gggtttggac tctgcctaag gaaaaacttc ctggtatgcc tttttgctgc
     7201 aagagtaaac tatacagcgg gctaccgttc atgccgatca accaatcggc gatctggcgt
     7261 gcttgtgctt cttcaaattt tgggtaggtc tcttctgcat ctcgtaactg ctggaatccc
     7321 tgataaatgg cttctttttc taaggagttg atccaaagtc ttttaaatat ctttccatga
     7381 tcggcgcctg cttgttgaat gatcgtccaa gctatcagtt ccccttcacg atcgctatct
     7441 gtcgcaataa taatggtgtc ggccgattga agttgctgtt tcaccacctt gaattgcttt
     7501 ttcttatctt taggaacatg atagtgataa tgtgtaggga aaataggcaa atgctctaat
     7561 gaccattgtt tccaattttc atcgtacata tcgggcgaat ctaactcgac taaatgacca
     7621 aaaccatacg tgatcacagt ttctcctgaa aacaatcgat cttggatctc aaaataccca
     7681 tcttttcggg tagctttaga aaagctatcg gcataagcct tcgcttgtga cggtttttct
     7741 gctaaaataa cggttttcat catgacgatt tcactccctt tcttgctgtc taccttgttg
     7801 aaacaacatg gcacgttttt gctttctgat ctcttgttgg tagatttcat gcttcaattg
     7861 atcattccaa tcctttttta cttcaggtac aaattcatgt tcatgatttt tatagccttt
     7921 gtagtattgt tctgctttat aagagaaaag aatctgagca ttaatatccc ctttttcttg
     7981 ataaaaacga tcaaaatcgt acgtaccatc cacgcgttca caagctttta attcttttgc
     8041 gacttctcta caaatatctt tcaaattaat agcttctatt gaatcgttaa cagaaagctg
     8101 ctgaccaaaa gacgcattta ctttgtgagc ggaaaccaat tggttcgtta attgcagatt
     8161 gttttctgtc ttgtgtatgg ccataatagc ttctggtgtg atcttttctt cttttgcgac
     8221 ttcttcataa atgactttgt attcttttgg cacttgccaa tcattagcga ttcctcgttc
     8281 acaacgtact ttctgattcg taaatggatc aactgcgccc attaattgtt ctttttcgta
     8341 gaaaatatgt cctgcacggt cattatccac acaaatctcg atggattgag gaaaggcttg
     8401 tttctttctc aattcagata ctgcctcacc aaaataatga ctgatcacat ggtgttttaa
     8461 cccttccatc gaaactaacc acgtatcgtt taactgatca cgatttagtg cagcatagct
     8521 aagtaaatca atactactct caaaaaattt taactgctta gggtcaccga tctttaggtt
     8581 aaagccatga ttcgctgtcg aatttttatc tatatgctta tatgtgcctc tctcaccaaa
     8641 acgttcataa tcaatgaacg ttccttgata actcgcccct actgtctcat ttgtctgtgg
     8701 atctttccat aaaaaggcga ccgcatgatc taaacgatcg tctcgaacta agaaattttg
     8761 ccgttcatgt tttggcttta gttcggcaat caacccttct ttttctgcat aatcaatcag
     8821 ccactctggt agttttcgtt cttccttcag ataattcctt cctttttctg tgagtggttg
     8881 ataatcaacg gggtgatctt tgtacttttc tgtctcaaaa tgaagatttt ccactcgttc
     8941 tgttttgtct tcattcttta gaataaaatc agtggctgct ttaaattgtt gcttagggtc
     9001 ttggatctct ttgtctatga aaaaaagttt agctaattca ataatgtcgc cactaatatt
     9061 ttgactgttc caataaaaac gttgttttct tcgatcaaaa acaaatgaat catggtcttc
     9121 taagcgatag tctcgtcctt tattgactac agccattcca ttgtttcgag caaaatccac
     9181 tatatcgatc tgtttacatc gatcaataag actcattttt ttctcggtca ttttaaaacc
     9241 tcccaaaaga aaaaagacta tccgtttttc agatagtctt ttttacttgt tttatacacg
     9301 ttctttttgt gacaattgtt gttcataata gatttcattt tttcgttttg ccacttctga
     9361 cactgtctca agtgaaaatc ggaatgaatt tcctagcgca aattcttctg aaggtaacgc
     9421 cgtttggatc tcttttccat aactttttcc ttttaaaatt acttgattat gctgtggatc
     9481 ttcaataaac gccgcatcct tcaaatgtaa cgttcgaccc gtatcttctt ccattacttt
     9541 cccgtctttc ttgtcgatcc aaagtgaacc aacaggaaca atattctttt gttctgccgt
     9601 ttctacagta tttttatgct cctgcttcac ttctgctggt tggatctctg gttctctttc
     9661 tctgtattga ttgatttcat tagaaatggt ttgaatgaat tcatttgcga cacggctgac
     9721 atcattcatg attttcccac gttccgcagc ttccttatca tccaacgtct gaccattctt
     9781 cgtccatgct gcaatataag gaatagcttt ctccgaagtg tccattccaa aatgtttgca
     9841 cacaatataa gaagtcattt ctgcctgcaa ttctttagtt gcggtatcta attctgaaaa
     9901 tttgtgcatt cttgcatggg ctaattcatg aatacttgta gctaaatgct gcgtaggagt
     9961 atttcgagta ttcatgacga tttcttcttc gccagtaaat tcgttacgaa catatgcccc
    10021 ccgtgcttgt cctaattctc tttgatagac ttcactttct gtcatatctc gaatttgtat
    10081 tccaatattg ttggctaccg cttgaatccc agcttgaagt tctgactgtc cttttttatc
    10141 caattgaaaa tcaaagacac ggttcggaaa gattttcgga tagtcttcag gttttaactg
    10201 tgtttgtgtg atatcaaaca ctttttcaat cgtgtagtaa ggttttttga tcacttcgat
    10261 ttgaccactt tcgattcgtt ttttatcttc tggtgtagct tctgtttctc gaacaaagcc
    10321 acgtcctttc tcataaaatc ctttgaccgt ttttctgaca aagatcttgt tcccttgttc
    10381 cccttttttg atatagattc cttctttttt caacgcagca cgacctaaac aagccgttgc
    10441 acctggaaat tgttcctcaa tcaataattg atttctaaag gatcgttctg gaaaacgaga
    10501 cataaaattc aaaagttctt tcacgtcttc aggagaatgg gtatgctttt tcaccgcatc
    10561 cagtgcctgt tgcgaaaggg atttgacttc ttgcttaatc tcttctgccg ttcgtttttg
    10621 atagcccttt tgttttgttt ttgtttcttt cgactgcatt tgttctttcg aaacaggttc
    10681 atttttgacc gtttgttttt gttccttttc aggtacttgt tcgcacaatt ttgctaactc
    10741 taaatagatg ggttgattac tgttgacgcc atcccctaga tccattcgga tctgatcgac
    10801 tacttctccg ttcttcactt cttcaaaata gaatttataa taacctgctt ctgcggcgta
    10861 ttggttatcc aacgttttga gttgatcgat caattctaat gttacgatct ccccaccata
    10921 acttttcaat cctttttctg gatcattttc gttgaattct actcgataga aatgatcgtt
    10981 ttctttctcc ttcaaagaat cagttggcgc agcctcttca agtaattcac gaacagctgc
    11041 tttttcctct tccgacgtgg cttggtattt ttttgattcc tcaaatcccc cgttttcatt
    11101 cagttctaaa gcaacttttc gatagtcttt caaaaaataa atggttggat tttttttcca
    11161 ttgcgtcact cgtccgctgt caataattgc ttgtacttca ggaagaagat tttcttttcc
    11221 tttttctgat ctttctttca gctgttctaa tgtttccaat ttccgttggt attttcggat
    11281 cgtagcggct gaaaaagtcg attcaccttt ttcaaagcgt tcgatttctt ctttgattct
    11341 tggaatattt tcgattgtca tatccaggcc gccgtacttg ttatagccca tttcctttag
    11401 ctcttcttgt ctttcaagtt tttctacctg tttttcttgt tgcttgatct cttctctcag
    11461 actatgcact ttgttttcta aacgttcttg ttgcctaaac caagaatgcc catttctctt
    11521 atcgttcatt ggctgaccgt tggtctttct ctggtgttca aacatcttct caatttgttc
    11581 gtttaattca ttttgcaatc gtactaattt tcttttggat tttcccaagc gactattgtc
    11641 tagtggttct ttttcttctg ccatgtc
//
LOCUS       DARBET010000079        11574 bp    DNA     linear   BCT 07-NOV-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4027
            SAMEA114569841-rid20112183.denovo.081, whole genome shotgun
            sequence.
ACCESSION   DARBET010000079 DARBET010000000
VERSION     DARBET010000079.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA114569841
            Sequence Read Archive: ERR12203666
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 11574)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11574)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001972618.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001972618.1
            Genome Coverage       :: 132x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/07/2023 13:18:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,041
            CDSs (total)                      :: 2,975
            Genes (coding)                    :: 2,908
            CDSs (with protein)               :: 2,908
            Genes (RNA)                       :: 66
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 67
            CDSs (without protein)            :: 67
            Pseudo Genes (ambiguous residues) :: 0 of 67
            Pseudo Genes (frameshifted)       :: 26 of 67
            Pseudo Genes (incomplete)         :: 28 of 67
            Pseudo Genes (internal stop)      :: 20 of 67
            Pseudo Genes (multiple problems)  :: 6 of 67
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11574
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA114569841-rid20112183.denovo.081"
                     /isolate="VRE4027"
                     /db_xref="taxon:1352"
                     /country="Denmark"
     gene            382..876
                     /locus_tag="SCJ86_002344"
     CDS             382..876
                     /locus_tag="SCJ86_002344"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348741.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEG1169866.1"
                     /translation="MTEAESALSQAEANPTKENYATASAAIQALPENKQELTSRLAAI
                     DSTIKAKEAEEERKKQEEIARQQAEQAQQEAIAQQQAEQARQEAIAQQQAEQARQQAA
                     AEQASQQAQAAAPEQNNEQTVYVTPTGSKYHTHKCGNGTYTPATLSEAQARGLTPCSK
                     CYGN"
     gene            complement(1038..2210)
                     /locus_tag="SCJ86_002345"
     CDS             complement(1038..2210)
                     /locus_tag="SCJ86_002345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333676.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class C sortase"
                     /protein_id="HEG1169867.1"
                     /translation="MNKSRIRILDIFMAIILVVGIGIFSYPFVEDSLNDFLAQQMIIH
                     YQKQASNKNSEEIKKQQEKMTKKNQQLAEQNVSPGIGSFNQAVDAKALKDLPSNAFFM
                     EHMLGVIEIPKINVSLPIFDQTTEIFLQKGTSLLEGSSYPTGGKNTHAVLSGHRGLPE
                     AKLFTDLPKLKKGDQFFIQINGKTLAYQVEKIQVVLPDEVDSLGIQKGRDLVTLLTCT
                     PYMVNTHRLLVTGHRIPYQAKEAKKAIQGIDQWKKWKFFIWFIGILLGSIGLVWLLIA
                     YLDSLAIAKRNYPLSFYVKNTNGRPIEGMVFSVKTLNGKHYITREKVPFVKASDEYGL
                     VRFSDLKGRNYRLQHEELLLKIHVKHKHSKQFSMKLKKGRYKLRKEKEVYYLIEKE"
     gene            complement(2416..3231)
                     /locus_tag="SCJ86_002346"
     CDS             complement(2416..3231)
                     /locus_tag="SCJ86_002346"
                     /inference="COORDINATES: protein motif:HMM:NF033902.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SpaH/EbpB family LPXTG-anchored major pilin"
                     /protein_id="HEG1169868.1"
                     /translation="MTGFDENTGVALYDPAESALTFTTDTEGSISVNGLIDGNYTLVE
                     TKAPDGYQLTNPQKETTFIVKNGKLQQTTGIIHGADEANLGNPNGVVNRENTTTDNTI
                     TVENVQNYGDFHFIKQDVNTQEKLADAEFSILRAKDPTVKLMKKIANLASGEYPYAWS
                     DEVTGSDWEEVKLISDSEGKFSIEGLKQGAYCLQETKAPEGYALPKNEAAYTEFTVDD
                     DPTTSDETTIDNQPKGILPSTGGMGIIVFILVGTALVGGAVIYFKKRHQETEA"
     gene            complement(3381..3827)
                     /locus_tag="SCJ86_002347"
     CDS             complement(3381..3827)
                     /locus_tag="SCJ86_002347"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell wall anchor protein"
                     /protein_id="HEG1169869.1"
                     /translation="MKNIKTKIWTFLGTAIMLLPFVLGLGTAEVSAAVSPTPENVTVN
                     LHKLKFTSAPENQINNGTELTFPNSEPLNGVEFNVYDITATYYPSKDTAVPADATPFA
                     SVTTSGEGLANLTLPGKSDGKDAVYVFVETPKPGVETSPNIVLSLP"
     gene            complement(3824..4828)
                     /locus_tag="SCJ86_002348"
     CDS             complement(3824..4828)
                     /locus_tag="SCJ86_002348"
                     /inference="COORDINATES: protein
                     motif:HMM:NF037639.1,HMM:TIGR04226.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="isopeptide-forming domain-containing fimbrial
                     protein"
                     /protein_id="HEG1169870.1"
                     /translation="MTDSSNHKLTTIHLYPKSEQKIPAALTLTKTVENKQTDFADGDK
                     VPYLITVTIPENINEIGTYTIKDTADPQLWLEPETIDFLIGGDKIHTFHTQKIKHGFV
                     LSDSGKDLSSFAGKKLTIRYQMTLKENSEKTTFDNDVRLTTDNQTVETHLAIQTGGKQ
                     FVKVDRQTKQKLADAQFLVKKNDQYLAQENGINHWVAKDDSRATIFTSSKDGTFSVHG
                     LSFGSYELVEIKPPKGYILSQSTILFEVEKGSYDPLHPIPLEIINEPKANPTNNGKTP
                     VQMQTNGPSNEKNGGSFPKTSEEMLGGFSILGLLLVLSTGTAWFYKKQQKGNNRREIK
                     "
     gene            complement(4868..5197)
                     /locus_tag="SCJ86_002349"
     CDS             complement(4868..5197)
                     /locus_tag="SCJ86_002349"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002352916.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell wall anchor protein"
                     /protein_id="HEG1169871.1"
                     /translation="MFESDKMPSISQNDGINIPDNFQGVKGLNGVTFAIYDVSDEFYK
                     LRSEGSSVEDAQRKLAQKSDSEKILAENVTKTVDEEEGIASFSASDKDEQGRDAVYRF
                     TEIKTSD"
     gene            complement(5323..7650)
                     /locus_tag="SCJ86_002350"
     CDS             complement(5323..7650)
                     /locus_tag="SCJ86_002350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303963.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LPXTG cell wall anchor domain-containing
                     protein"
                     /protein_id="HEG1169872.1"
                     /translation="MKMIKKTQNKIQRICQKHFVSRLKAVSKRKYLHPIMISIILIFT
                     FLGSLSSIYVNVYTETSNFANQTTEETNSPPYSEIQSIESTNQSSNNETTSTTSSTQL
                     IDPTEKEITAAKAAGEKAYSETGRSQTFSTVAATANFNGHTIPDGYTVIYAKDGVIVA
                     VNGSMSADKTALQGVTKAIAFIDNRPDIHNLFIGSTVNGTTITFPKSIYMGVATAGNT
                     IDSVTPNADKSYSFTPSTWGGLPAGGGMSTFSIGIQLANDIPFTGYSIKNGQIVDGKG
                     NVSNFPTYQAATVQVNANRTGTFSYTVNGGAAKIGTYGTTLSGIHVGDKVVITPTAVT
                     GYSFAQTNGSFTALESNVDTVTYTGKQTPITIKYQDYFGQMIAPSKIMNLTYGSAAQD
                     LTTNTPTINGYTFTAVSAIETKNQSAMSVLASLDTNGNVIVKDANGKQISEVILYYKT
                     NATVSINANGSKYYDGLSVVPIVKYAFNDKTESTSLLNNDLSIDQITWNPTDFKAMNE
                     KGVEVTAPTEIGTYTSWQLTKSGLAKLAARTNYLFTIVQTSDVYTIKQISGAVELSGS
                     KTYDGKAGVPSIHVKLAEGVTSSLTPVQVALSSMDYTADAQSAKNMVNAGSYTIKLTN
                     SGIDKVKAANPKLSFTDIANTSGTYTIYKANAVITVDDAAFNYDAQSHSISVGNLHVT
                     GVVLGESLDYTLTVNSRTDIGSQIVTISLGEGSVNNNYTIIATDTAKLTINPTLLLPS
                     TGGIGLLPFVFLGLIFIFSGFFYFIHRKKKAGEQR"
     gene            complement(8493..8786)
                     /locus_tag="SCJ86_002351"
     CDS             complement(8493..8786)
                     /locus_tag="SCJ86_002351"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016922289.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2087 domain-containing protein"
                     /protein_id="HEG1169873.1"
                     /translation="MVYTYTEQEKMKVLDTFMNDGKVKQVPSSTKKKYILLKEVLKRF
                     DHGVTYTETEVNSILLNVFSSGDYVEQRRYLITFGFFKRSSDGRAYQMMGIEN"
     gene            complement(9189..10367)
                     /locus_tag="SCJ86_002352"
     CDS             complement(9189..10367)
                     /locus_tag="SCJ86_002352"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002354626.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HEG1169874.1"
                     /translation="MEKTDRFKKQISSTGRITWYFQAYRTTRRGFNSKREAQLAYLEM
                     EKQQRLKKKIVASNDKFSVVGEQWFQYYRSLNEQKEITYDKREEQLKIIVRWIGDKKL
                     SDLTPECLQELLFTLKECGVNGTNVGYAKNSLQSLIQVLNMIFKYCQRKNLISENPMK
                     SVRMPKYQLTVTDLKEAINNIEDKFLTVDELRTFLNYGIVHEELPMSVLFHVLFYTGC
                     RVSEALALQPGDIDFKRNEILFYKQTAVKGKSKDFRIETTKTVSSARRVLVTPLVMEK
                     LQQLIKALGEMRQHYRFIIEETYLFVYLDSSKRGVPYRREYVNDHVKRCVNRSGINKP
                     FHTHLARHTMASLVAEHCSWEVLKDRLGHTDQTTSKIYRHLTSNEKVKPLIAFSSLES
                     "
     gene            complement(10471..10785)
                     /locus_tag="SCJ86_002353"
     CDS             complement(10471..10785)
                     /locus_tag="SCJ86_002353"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300928.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HEG1169875.1"
                     /translation="MELEVKTNVNSLEIEFYSILQKKLEDYTEKLLAVSHLPLVLTNT
                     DLKRLFQISDSTLNRLVKLGEFPPCWYGIRGHYFRDDVLEWMRKSDSNDYREKLRLLR
                     SL"
     gene            10892..11110
                     /locus_tag="SCJ86_002354"
     CDS             10892..11110
                     /locus_tag="SCJ86_002354"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016613890.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEG1169876.1"
                     /translation="MKQGIADIKIIKEILEKSTANAIAFGTGINLSTVKKLKSGERAE
                     EKLNLADAIKITEFGMKNMPTKIEIWKS"
     gene            complement(11105..11500)
                     /locus_tag="SCJ86_002355"
                     /pseudo
     CDS             complement(11105..11500)
                     /locus_tag="SCJ86_002355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317574.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
BASE COUNT         3352 a         2264 c         2019 g         3939 t
ORIGIN      
        1 attgcatact taataacaga actatagcaa aaaatcccgt gtatcaacac gggattagat
       61 taagtttcct caatcactat tcataacaca attattataa caagtaagta aacaattgaa
      121 aaggacattt attgaaaacg gttgtttttt acttaaggag gaaatttagt gaaaaaatta
      181 gttgcgttgt ttttggtcgc aggaagtttg ttgagtgggt gtggtgctac tacgtctact
      241 actcaaagca caagttcagc tcaggcttcc agcagcagta ccacgatctc cgaaagtacg
      301 gcagattcat cgaaagaaca aattgaaaag aaaaaagaag aagagaaaca aaaagcggaa
      361 aaagctgcac tagaaaaaaa agtgacagaa gcggaatctg ctttgtcaca agctgaagca
      421 aatcctacaa aagaaaacta tgcaactgct tcagcagcta tccaagcgct acctgaaaac
      481 aaacaagagt tgacaagccg tcttgctgca attgattcca caatcaaagc aaaagaagca
      541 gaagaagaac ggaaaaagca agaagaaatt gcacgacaac aagcagaaca agcgcaacaa
      601 gaagcaatcg cacagcaaca agcagaacag gcgaggcaag aagcaatcgc acagcaacaa
      661 gcagaacagg cgcgacagca agcagcagct gaacaggctt ctcagcaggc acaagccgca
      721 gctcctgagc aaaataatga acaaactgtc tatgtcacac cgaccggttc aaaataccat
      781 acacataaat gtggaaatgg tacgtatacc cctgccactt taagcgaagc tcaagctcgt
      841 ggtttaaccc cttgctccaa atgttatgga aactaattca aaagttatac tataataata
      901 aaattcttct gaaacataaa aaaatagaag ctggcgttcc agatagtaaa ccaaaaatag
      961 cagtttacac gtccatttcg tcagcttctc tcttttttca tttagtctgt tccctctgct
     1021 tttttggaca tcttcattca ttctttctca atcaaatagt acacctcttt ttcttttcgc
     1081 agtttatatc ttcctttttt caatttcatt gaaaactgtt tcgaatgttt atgttttaca
     1141 tggattttaa gcaggagctc ctcatgttgc aagcgatagt tcctgccttt taaatcagaa
     1201 aatctcacta gtccgtattc atcagaagct ttcacaaatg gaactttttc tctagtaata
     1261 tagtgtttgc cgttgagcgt ttttacagaa aagaccattc cttcgattgg tcgtccgttt
     1321 gtgttcttta cataaaaaga taaaggatag tttcgcttag cgatggccaa agaatctaaa
     1381 taagcgatga gtagccaaac taagccgatt gatccaagta gtataccgat aaaccaaata
     1441 aaaaatttcc attttttcca ttgatcaatt ccttggatcg ctttttttgc ctcttttgct
     1501 tgataaggaa tacgatgccc cgtcacaagc agtcgatgtg tattgaccat ataaggagtg
     1561 caggtaagca aagtcacgag atctctgcct ttttggatac ccaaagaatc tacttcgtca
     1621 ggtaagacca cttggatttt ttctacttga tacgccaatg tctttccatt gatctggatg
     1681 aaaaactggt ctcctttttt caacttaggc aaatctgtaa ataattttgc ttctgggagt
     1741 ccacgatgcc cagaaagaac ggcatgggtg tttttcccgc cagtcggata agaacttcct
     1801 tccaataatg acgttccttt ttgcaaaaat atttccgttg tctgatcgaa tataggtaag
     1861 ctgacattga tttttgggat ttcgatgact ccaagcatat gttccataaa aaaggcatta
     1921 ctaggtaaat cttttaatgc ttttgcatca actgcttggt tgaaactgcc tatccctggt
     1981 gatacgtttt gctctgccaa ctgttggttt ttctttgtca ttttttcctg ctgttttttt
     2041 atttcctctg aattcttatt cgatgcttgc ttttgataat ggatgatcat ttgctgggca
     2101 agaaaatcat tcagtgaatc ttctacaaaa ggataagaaa agatcccgat cccaacgact
     2161 aaaatgattg ccatgaagat gtccagtatt cttattctgc ttttattcat catgtccacc
     2221 tattttctaa tatgtatatg aagaggttgt gaaatcagcg ccttgacctg agtattagcg
     2281 aagcaaacgg caaaatagtt cctcctattt tgccgtttgt tgagcgaata ccaagagacc
     2341 gaccacttgt cacaaccttt ctccccattt gaatcactat cgtttattta tcgtatgatt
     2401 tttgtttcca atagtttaag cttctgtttc ttgatggcgt tttttgaaat agatcactgc
     2461 tcctccaact aaagcagtac ctaccaagat gaatacgatg atccccatac caccagttga
     2521 aggcaagatt cctttaggtt ggttatcgat cgttgtttca tcagaagttg ttggatcatc
     2581 atctactgta aattcagtgt acgcagcttc attcttaggt aatgcgtagc cttctggtgc
     2641 ttttgtttct tgaaggcagt atgccccttg tttcagccct tcgattgaaa atttcccttc
     2701 tgaatcagaa atcaatttca cttcttccca gtcagaacct gtgacttcat ctgaccaagc
     2761 atatgggtat tctcctgagg caaggtttgc tatttttttc attaatttaa cagttggatc
     2821 ttttgctctt agaatagaaa attctgcatc ggctaatttt tcttgcgtgt tcacatcttg
     2881 tttgatgaag tggaaatcac cgtagttttg gacattttct acagtaatcg tgttatccgt
     2941 agttgtattt tcccgattga ctacaccgtt tggattccca agattggctt catctgcgcc
     3001 atggatgata ccggttgttt gttgcaattt tccgtttttt actataaaag tagtttcttt
     3061 ttgtggattg gttaattgat aaccatctgg tgcttttgtt tcaactaaag tataattgcc
     3121 gtctattaat ccattgacac tgatggatcc ttcagtatca gttgtgaatg tcaacgctga
     3181 ctcagcagga tcatagagag cgacaccagt gttttcatca aaaccggtca cgaattgatt
     3241 ctctgaattt ttgatctgga attgcgcccc tgctaactta tctacccctg aaaagttccc
     3301 agctttctgg attttgatat tgctttcttt gatgacattt ttagcatata ggtgaatcgt
     3361 gtctaaaggt ttgtcagggt ctagggtaag cttaacacga tatttggcga cgtttcgaca
     3421 ccgggctttg gtgtttctac aaagacgtat acagcatctt tcccatcaga tttacctgga
     3481 agagtcaggt tagctaatcc ttcaccagaa gtcgttacac ttgcgaaggg tgtagcgtct
     3541 gctggaactg ctgtgtcttt acttggatag tacgtagcag tgatgtcata tacgttgaat
     3601 tcaacgccgt tcaacggttc gctattcgga aatgtcaatt ccgttccgtt gtttatttga
     3661 ttttctggtg ctgaagtgaa tttcaattta tgcaaattaa ccgtaacatt ttctggtgta
     3721 ggtgatacgg ctgcagaaac ctctgcagtt cctaggccga gaacgaaagg aagcagcatg
     3781 atagcagttc ctaaaaacgt ccatattttt gttttgatgt ttttcatttt atttccctcc
     3841 tattgtttcc tttttgttgt tttttataaa accaagctgt ccctgttgaa aggactagca
     3901 ataaccctaa aattgagaaa cctccaagca tctcttcgct tgtttttgga aagcttcctc
     3961 catttttttc atttgatgga ccatttgtct gcatctgaac tggcgttttt ccattattcg
     4021 tcggatttgc ttttggttcg ttgatgattt ccaatggtat aggatgaagc ggatcataac
     4081 tgcctttttc aacttcaaag agaatggttg attgactcaa tatatatcct ttaggcggtt
     4141 taatctccac tagttcgtaa cttccaaaag acaatccatg gacagaaaat gttccatctt
     4201 tactggaagt aaaaatcgtt gctcttgaat catctttggc tacccaatga tttatcccgt
     4261 tttcttgcgc taagtattgg tcattttttt tgactagaaa ttgagcatct gctagttttt
     4321 gtttcgtttg ccgatccact ttgacaaatt gtttgcctcc tgtttggatg gcaagatgtg
     4381 tttctactgt ttgattgtca gtcgtcaaac gtacatcgtt atcaaatgtc gttttttcac
     4441 tgttttcttt caatgtcatt tggtaacgga tcgttaattt ttttccagca aacgaactaa
     4501 gatcttttcc agaatctgat aagacaaaac catgttttat tttttgtgtg tgaaatgtat
     4561 gaatcttgtc tccaccaatc aaaaaatcga tcgtttctgg ttctagccat aactgaggat
     4621 cagctgtatc tttgatcgta taggttccta tctcgttgat gttctcagga attgttacgg
     4681 tgatgagata agggacttta tccccatctg caaaatctgt ctgcttattc tctactgttt
     4741 ttgttaatgt gagtgcagca ggtatttttt gttcgctctt tggatacaag tggatggtcg
     4801 tcaatttatg attagatgaa tctgtcacag gaagcacaac gacaaatggt gctgattttt
     4861 cttttactta atctgaagtc tttatttccg taaatcggta aacagcgtca cgaccttgct
     4921 catctttgtc cgatgcacta aaagaagcaa tgccctcttc ctcgtctact gtttttgtca
     4981 cattttctgc cagtattttt tcactatcac ttttttgtgc tagttttctt tgtgcgtctt
     5041 ccacactact tccttctgac cgcagcttgt aaaattcatc cgagacatca tatatagcga
     5101 aagtaacgcc gttcaaccct tttactcctt gaaagttatc cgggatattt attccgtcgt
     5161 tttgactgat acttggcatc ttatcgcttt cgaacattcg attatgcaaa gtaaatgtaa
     5221 taggtgattc tctagctgac acagtcggag aacctataaa aaagaccagt aggataaaaa
     5281 agagaacagc aaatatatcc acacgtacat tttttgtttt tttcatcgct gttcacctgc
     5341 ttttttctta cgatggataa aataaaagaa gccagaaaaa ataaagatca gtcctaaaaa
     5401 gacaaagggc agtagaccta tcccacctgt cgaaggaagc aagagagtcg gattgatcgt
     5461 caacttagcg gtatctgttg cgataatcgt ataattattg tttacacttc cttcgcctaa
     5521 actaatcgtt actatttgtg atccaatatc tgtccggctg ttaactgtta gggtataatc
     5581 caatgattcg cctaagacaa caccggtaac gtggagattt ccaaccgaaa tcgagtgact
     5641 ttgggcatca taattaaagg cagcgtcatc gacagtaatc actgcgttag ctttatagat
     5701 ggtataagtt cctgatgtgt tggctatatc agtaaatgac aacttgggat ttgctgcttt
     5761 gactttgtct attccgctat tcgttagttt aattgtatag ctgccagcat taaccatatt
     5821 ttttgctgat tgtgcatctg ctgtgtaatc catactgcta agtgccactt gtactggagt
     5881 aagcgaacta gtgacaccct ctgctagctt gacatggatt gacgggacac ctgcttttcc
     5941 atcatatgtc ttactgccac tgagctctac cgcaccgctg atttgcttaa tcgtatagac
     6001 atcagatgtc tgcacgatag taaataaata gtttgttctg gcagctagtt tagctagtcc
     6061 gctttttgtc aattgccaag acgtataggt tccaatttca gtaggcgcag ttacttcaac
     6121 tcccttttca ttcatcgctt tgaaatcagt tggattccat gtgatttgat caatactgag
     6181 atcattgttt aaaagggatg ttgattctgt tttatcatta aaggcatatt taacgatcgg
     6241 tacaacagaa agaccatcat agtacttact gccatttgca ttgattgata ctgttgcatt
     6301 tgtcttataa tataggatga cttcagaaat ctgttttcca tttgcgtcct ttacgattac
     6361 atttccattt gtatctaaac tagctaaaac actcattgct gattggttct ttgtttctat
     6421 cgctgaaaca gctgtaaagg tatatccgtt gatagtaggc gtattagtcg tcaggtcttg
     6481 tgcagcactt ccgtaagtaa gattcattat tttagaagga gcaatcatct gtccaaaata
     6541 gtcttgatac ttaatagtga ttggtgtttg tttgcctgta taggtcaccg tatcgacgtt
     6601 actttctagg gctgtgaaag aaccgtttgt ttgtgcaaaa gaatagcccg ttactgctgt
     6661 tggagtgata accactttgt cacccacatg gattccagat aatgtagtcc cgtacgttcc
     6721 gatttttgct gctccaccat tgactgtata agaaaaagtt cctgtccgat tcgcattgac
     6781 ctgaaccgtt gctgcttgat aggttggaaa atttgaaaca tttcccttac catcaacgat
     6841 ctgcccattt ttgatcgagt accccgtgaa tggaatatca tttgctaact gtataccgat
     6901 tgaaaaagta ctcatacctc ctccggcagg aagccctccc catgttgagg gtgtaaaaga
     6961 ataactttta tccgcattgg gtgtaactga atcgatcgta tttccagcgg tagccacccc
     7021 catatagata ctttttggga atgtgatagt agtaccattt actgtggagc caataaacag
     7081 attatggata tctggacggt tatcaataaa agcgattgct ttggttacgc cttgtaacgc
     7141 agttttatcc gcactcatcg atccgttaac cgcaacaatg actccatctt tggcatatat
     7201 cactgtatat ccatctggta tggtatgtcc gttgaaattc gcagtggccg caacagtact
     7261 aaatgtctgg cttctacctg tttctgaata agctttctcc cctgctgctt ttgctgctgt
     7321 gatttctttt tctgtcggat caatcagttg agtcgaggat gtggttgagg ttgtctcgtt
     7381 gttgctagac tgattggtgc tttctatgga ctgtatttca gaatagggtg gactatttgt
     7441 ctcctctgtc gtttggttag caaaatttga cgtttctgta taaacgttta cataaatact
     7501 tgataagctc cctaagaaag taaaaatcaa gatgatcgaa atcataatgg gatgtaagta
     7561 ttttctttta gatacagctt tgagtctaga aacaaaatgt ttttgacata tacgttggat
     7621 tttattttga gttttcttaa tcattttcat ccctcctctc aataaaaaat catcaaataa
     7681 tcctttacac gttattctaa atttaatttt tattatttta ttgatgtata gaaagtttgg
     7741 ggcttttttt ctctttttat tctaaggtta ttttagtctt gtcctttaat acaaagattt
     7801 tatttttaaa taaaaaatac tcttttttta cgattattga aaaaaaaaga taaaacttga
     7861 ttaattgaag tattgcttaa atttggaagc tatgagggaa gaataaaaga gttaggacgg
     7921 gattatctac tatttttctt tgttttaaat atttgttatc tttgaacata aaagagaaat
     7981 gtgtcgactg cagtgttgtg tccctcatca cgaataaacg gtgttcaatt agctgatctt
     8041 gtattcactt aaaaaatggt aaagtacgag ttgctattcc ccattttcaa ttttctaacg
     8101 ctcaggacaa aatactgttt tcacaacctc attggaataa aagcatatat attgtttcaa
     8161 gaacgaaatc ttgttatctt tttaaagggc atctgtccaa aatctaattt taggaggtac
     8221 gttttggtat ttattcgtac aaataaaaga gaattatctt tattcatgta acgcatccaa
     8281 ttgatttttt catgtacgct cctggaacga ttttactgac gattgcttat ttaactgcga
     8341 atcgctcatt agcttttatc atagctatcc atattttgaa taacgttctt ggctttgtat
     8401 tatagataca gatgacaaaa aaagttcaag tatgcggtgt agaaccaata tcgcatactt
     8461 gaactttttt caatgagtat aaattaaaac ttctaatttt caattcccat catctgatat
     8521 gcccgaccgt ctgaacttct tttgaaaaag ccaaatgtga ttagataacg tctttgttcg
     8581 acataatcgc cactagaaaa tacgttcagc agtatcgaat taacctctgt ttctgtataa
     8641 gtcactccat ggtcaaagcg tttcaagact tctttcaata ggatgtattt cttttttgtt
     8701 gaacttggta cttgttttac ttttccatca ttcatgaatg tgtctaaaac tttcatcttt
     8761 tcttgctccg tataagtata aaccatcttc tttctccttt tgattctaag aatactccat
     8821 ttatgttaga atattctgga ttttttatta aagcggttac tcttaagaaa gatattatca
     8881 tagtgctctt ttttttacta ctacttatag gtagatttat tctaatccat tattctcccc
     8941 taccaataat taaagaaaaa actggatcag ggtgttcaaa ttacatgcta agaaaaaaga
     9001 acccttgaat accaagggtt ctttctcagt ctgataacaa tgattaacga cggatttctt
     9061 tgatacttgt gaaaagtcgt taaagtattg atataaaagg atttttatta cctctgacta
     9121 catattgact aatttgttaa aaaatcgttt ttcaagaatc ctgtaatacc agtgctttca
     9181 ccgttttttt aactctctaa agaactaaaa gcaatcagag gttttacctt ttcatttgac
     9241 gttaagtggc gataaatttt cgaagttgtt tggtcagtat ggcctaagcg atctttcaag
     9301 acttcccagc tacagtgttc cgctactaag cttgccattg tatgacgagc taaatgagta
     9361 tgaaatggct tattaattcc tgagcgattc acgcaccgtt tgacgtgatc atttacatac
     9421 tctcgacgat aaggaacacc acgtttacta gaatcaagat acacaaacaa atatgtttct
     9481 tctataataa acctgtaatg ttgcctcatc tcacccaaag ccttaatcaa ctgttgtagc
     9541 ttttccataa ctagaggagt tactagaact cgtctagcag aactaactgt cttagtagtt
     9601 tcaattcgaa aatccttact cttaccttta actgccgttt gcttatagaa cagaatttca
     9661 tttcttttga aatctatatc tccaggctgt agagcaagcg cctcgctgac tcggcagcca
     9721 gtgtaaaata gaacatggaa aagtacagac atcggtaatt cctcatgaac aatgccataa
     9781 ttcaaaaaag ttctcaattc atcgacagta agaaacttgt cctcaatatt atttatggct
     9841 tcttttaaat cagttaccgt taactgatat tttggcattc tcacagactt cattggattt
     9901 tcacttatca aattctttct ttgacaatat ttaaaaatca tatttagaac ttgaataagt
     9961 gattgtaaac tatttttggc atagccaaca tttgttccat taacgccaca ttcctttaag
    10021 gtaaaaagca attcttgaag gcactctggt gtcaaatcag acaacttttt atcaccaatc
    10081 catcgaacaa taatttttaa ttgttcctct cgcttatcat aggttatttc cttttgttca
    10141 tttaacgaac ggtaatattg gaaccattgc tcacctacaa cactaaactt gtcattgctt
    10201 gcaacaattt ttttcttaag cctttgttgt ttctccattt ccagataagc gagttgcgcc
    10261 tcacgttttg aattaaagcc acgtcttgtt gttcgatatg cttgaaagta ccatgtgatc
    10321 ctaccagtag aactgatttg cttcttgaat cgatctgttt tttccattta ttgattcctc
    10381 ctattttgaa ggaacccaac aagcagaaac cctttggaaa tctttctatc tatgtcatca
    10441 ttgtaccgca tcttagaaag aaaatgtaac ttataaagat cgaagtagtc gcaatttttc
    10501 tcgatagtca ttcgaatcac tctttctcat ccattctaaa acatcatcac gaaaatagtg
    10561 tcctcgaatc ccgtaccaac agggaggaaa ctcaccaagt tttactaaac ggttcagtgt
    10621 actatcactt atctgaaata gtcgctttaa atcagtattt gttaatacca gaggtaaatg
    10681 actgactgca agcaattttt ccgtgtaatc ttccagtttt ttttgtaaaa ttgaatagaa
    10741 ctctatttcc agagaattaa catttgtttt aacttccaac tccatcaccc ttctctctca
    10801 aaatcaaaac gaccaccacg gtacccctta aaattgtaaa aaaacactcc agtactccat
    10861 caaaacggac tagaaaagag gaatttttaa tatgaaacaa ggaattgcag acataaaaat
    10921 aattaaagaa attcttgaaa aatctacggc taatgcaata gcattcggaa caggaatcaa
    10981 cttgagtaca gtaaaaaaac ttaaaagtgg agagagagct gaagaaaaat taaatcttgc
    11041 cgatgctatt aaaatcacag aatttggcat gaaaaatatg ccaaccaaaa tcgaaatatg
    11101 gaaatcctag ctcttccaaa gccatttgtc gttggtacca aacactgccg agacttttcc
    11161 caataatatc gccaatttca cgatcattaa gaccgatgaa gaaggacata aaaatgattt
    11221 cttgtctttg actaggtaat gctgcaatcg catttgccaa ctcctcattg gaaataaaga
    11281 ttttcatacc aaggacaata aaagtaccac tactattcaa atttggttcc aaataataga
    11341 actctgtacc taaccctttt tgttccaata tttccattga tatttgccgt tggcgaaatc
    11401 tagcgtactt cttttgaata tttaacgcct cattgcagac tactttctta caaaaactgt
    11461 caaacgtatg ttgaatgtgg gtttcctctg agtcgcccat aatattctcc ccctcaaaaa
    11521 attcattcaa ctcgttttac ttctatccct ttccattatt actacaatct agga
//
LOCUS       DARBET010000080        11513 bp    DNA     linear   BCT 07-NOV-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4027
            SAMEA114569841-rid20112183.denovo.082, whole genome shotgun
            sequence.
ACCESSION   DARBET010000080 DARBET010000000
VERSION     DARBET010000080.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA114569841
            Sequence Read Archive: ERR12203666
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 11513)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11513)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001972618.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001972618.1
            Genome Coverage       :: 132x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/07/2023 13:18:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,041
            CDSs (total)                      :: 2,975
            Genes (coding)                    :: 2,908
            CDSs (with protein)               :: 2,908
            Genes (RNA)                       :: 66
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 67
            CDSs (without protein)            :: 67
            Pseudo Genes (ambiguous residues) :: 0 of 67
            Pseudo Genes (frameshifted)       :: 26 of 67
            Pseudo Genes (incomplete)         :: 28 of 67
            Pseudo Genes (internal stop)      :: 20 of 67
            Pseudo Genes (multiple problems)  :: 6 of 67
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11513
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA114569841-rid20112183.denovo.082"
                     /isolate="VRE4027"
                     /db_xref="taxon:1352"
                     /country="Denmark"
     gene            complement(208..693)
                     /locus_tag="SCJ86_002356"
     CDS             complement(208..693)
                     /locus_tag="SCJ86_002356"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289239.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prolyl-tRNA synthetase associated
                     domain-containing protein"
                     /protein_id="HEG1169877.1"
                     /translation="MSRATEQEAYELLKKLGISFQKVDHPAITSVKNVPFSLPGPQVK
                     NLLLKSRKGKQIYLVILPDEKHADLKQLAEQLKEKRLSFVSEEQLPDLLGVPAGTVTP
                     LALMHDKEKKIQVVIDAAIDRQDTVGFHPNINTTTLIMDFSDFEKILVYLEHLPVYEN
                     L"
     misc_binding    783..1128
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00230"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="T-box leader; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="tRNA"
                     /db_xref="RFAM:RF00230"
     gene            1173..1814
                     /locus_tag="SCJ86_002357"
     CDS             1173..1814
                     /locus_tag="SCJ86_002357"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293344.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter permease"
                     /protein_id="HEG1169878.1"
                     /translation="MEYLIEVMPQLLVGALTTLKLFLFTLIGSLPLGIVLALGLSLHF
                     KPLRYLLQTYVWVMRGTPLLLQLIFIFYGLPTIGIVFDRFEAALIAFILNYAAYFAEI
                     FRGGIQSIPQGQYEAAQVLQLSPFQTVTKIVIPQVTKIVLPSVGNEVINLVKDSSLIY
                     ILGLGDVMRAGKIAMERDATLLPLLGVAIIYLTLTGILTIIMKQVEGKLNYYR"
     gene            1833..2468
                     /locus_tag="SCJ86_002358"
     CDS             1833..2468
                     /locus_tag="SCJ86_002358"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter ATP-binding protein"
                     /protein_id="HEG1169879.1"
                     /translation="MIELKKVNKAFGEKKIIKDLNLIIPDGKTLAIVGPSGGGKTTLL
                     RILAGLESSDSGEFYLDGVPFNPFELKMKEQVVGVVFQDFQLFPHLNIFENITLAPKM
                     VLKQTKEEYTKKAEELAASLGISDLLNIYPYQLSGGQKQRVAIARALAMNPRVLAYDE
                     PTSALDPELRQQVERLILSLKEDGVTQIIVTHDLVFAENVGDQLLKVSPIH"
     gene            2486..3301
                     /locus_tag="SCJ86_002359"
     CDS             2486..3301
                     /locus_tag="SCJ86_002359"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289242.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter substrate-binding
                     protein"
                     /protein_id="HEG1169880.1"
                     /translation="MKKTSFLFALIAGLLLFAGCSNKKTSADNWATINEEKKIVIGLD
                     DTFVPMGFRDKDGKLTGFDIDLAKAVFEQYGITVDFQPIDWSMKEFELNNGTIDLIWN
                     GYSKTPAREKKVQFTKPYMENDQVLITPKESGITNFGQMNNKRLGAQNGSSGYDVFTK
                     QPEVLKEIVKNQDAVLYDSFNEALIDLKSRRVDGLLMDKVYADYYLKQRNELEDFNIT
                     KGSYESEDFAVGARKSDSTLVKKINEALDQLEKTGEFQKISDKWFGEDVTPRN"
     gene            3620..5056
                     /locus_tag="SCJ86_002360"
     CDS             3620..5056
                     /locus_tag="SCJ86_002360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289243.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 1 protein"
                     /protein_id="HEG1169881.1"
                     /translation="MEHKELTSFPKDFLWGSASAAYQVEGAWQEDGKGESVWDRFVRI
                     PGKTFKGTNGDLAVDHYHRYKEDIALMKEQGLKAYRFSVAWTRIFPNGRGEVNQAGLA
                     FYERLIDELIENDIEPMLTLYHWDLPQALQDEYNGWESRQIIEDFTHYAETLFEAFRG
                     KVHYWISLNEQNIFTSLGYLLAAHPPGVTDPKRMYEVNHIANLANASVINKFHELEMP
                     GKIGPSFAYTPNYPIDSNPENVLAAENAEDLMANYWMDVYMWGKYPIAAMRFLEEKGW
                     APTIEAGDAELLESAKPDFLGINYYQTATNAFNPLNGVGAGKMNTTGKKGSSEETGVP
                     GMYKKVENPFVERTNWDWEIDPEGLRIGLRRITSRYRMPVLITENGLGEYDKLTEDKQ
                     IHDDYRINYLQSHVKAIKEAISDGAEVLGYCTWSYTDLLSWLNGYQKRYGFVYVDQDE
                     TQEGSLERYKKDSYYWYQKLIEENGENL"
     gene            5245..7692
                     /locus_tag="SCJ86_002361"
     CDS             5245..7692
                     /locus_tag="SCJ86_002361"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344000.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA translocase FtsK"
                     /protein_id="HEG1169882.1"
                     /translation="MAQKKRPTKKRKKTKKTKKQKQQQEQMLTIGVGFALILFAVFGF
                     LKLGFLGILIANGFRIIAGNTYQILCLLLAVLGFWIVIKNTEFSIGKNRRWFGGILFY
                     FGILLLLHAHLFGKLHTGEPNIMGTTWDLLASDIKQSQVDNNVGGGMIGAILYHFTYF
                     LIAQPGSYLAAVLLLAGGAFLFSNLEGYQLLNGIQSIGERVQELLEGDPAKQARKQAA
                     KEERMKQRAEAKEARRLAAKEAAEKEAAEYEKKKAVSQQRENTADEWQEPENTEPEQL
                     SFVPIDSFQENIHPANLEKPVPDTPKQTNTAEGFSDELPEDDGTSLEFEIEAEQENQD
                     YELPTVDLLDSIPTVDQSDEYKKIEKNIGVLEQTFQSFGVDAKVVKASLGPSVTKFEV
                     QPAVGVKVSKIVNLTDDIALALAAKDVRMEAPIPGKSLIGIEVPNGKISMVSFREIIE
                     AQPNHPDKLLEVPLGRDVSGRVQTADLSKMPHLLVAGSTGSGKSVAINGIITSILMRA
                     KPHEVKLMMIDPKMVELNMYNGIPHLLTPVVTNPRKAAQALQKVVQEMEERYEKFAAT
                     GVRNISGYNEFVQQKNLENGTKHPTLPFIVVIVDELADLMMVASNEVEDAIIRLAQMA
                     RAAGIHMILATQRPSVDVITGIIKANVPSRMAFAVSSGTDSRTIIDSNGAEKLLGRGD
                     MLFLPMGENKPIRVQGAFISDHEVERVVQFVTDQQEAHYEEKMMPTDEVETTGAPEQP
                     QDELFEEAKALVVEMQTASISLLQRRFRIGYNRAARLVDELEAHGVVGPSEGSKPRKV
                     FIEQEETSETLPDDNDM"
     gene            7922..8989
                     /gene="rlmN"
                     /locus_tag="SCJ86_002362"
     CDS             7922..8989
                     /gene="rlmN"
                     /locus_tag="SCJ86_002362"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629547.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (adenine(2503)-C(2))-methyltransferase
                     RlmN"
                     /protein_id="HEG1169883.1"
                     /translation="MEKQSIYGLTNEELINWFIENGEKKFRAAQVWEWLYQKRVSNFT
                     EMTNLSKQLIEKLSEHFIINPLKQMVVQEASDGTVKYLFELPDKNMIETVLMRQEYGL
                     SVCVTTQVGCNIGCTFCASGLLKKNRDLTAGEIVAQIMMVQHYFDERNLGERVSHVVV
                     MGIGEPFDNYDNVMDFLHIINDAKGLAIGARHITVSTSGLAHKIKEFANNGLQVNLAI
                     SLHAPNNEVRTSIMRINRSFPIEKLMEAVDEYLEKTNRRITFEYIMLNQVNDRPEHAQ
                     QLADLLKDKKKLAYVNLIPYNPVSEHDQYSRSPKADVLRFYDVLKKNGVNCVIRKEHG
                     TDIDAACGQLRSKQMKKTVAK"
     gene            complement(9182..10285)
                     /locus_tag="SCJ86_002363"
     CDS             complement(9182..10285)
                     /locus_tag="SCJ86_002363"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373950.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-containing alcohol dehydrogenase family
                     protein"
                     /protein_id="HEG1169884.1"
                     /translation="MSQSVFLPNYTIGTDAYEKANGIVKEYGTKVVLIGGKTALSKAE
                     NKIKVALTQEIEVVASLWYGGNSTYGNIEQLIQHESVQKADVLFAVGGGRACDTVKVI
                     GEKLAKPVFTFPTIGSNCSPVTAVCVIYNEEGVMDGLFFPSKPPVHTFIDTAIIAEAP
                     DKYLWAGIGDALSKEVESTFSARGDDLGYTDRLGIQIGKMCIDRLLTYGTQALKDSAD
                     DRPSYAIEQVVSDIIVTTGLVSVLVNNNYNSAMAHSFYYGTTVLEKMEGYLHGASVSY
                     GVLTLLMMDQQFDLFDKIYVFMKKNQLPVSLTDMNLNVTTDLEAILDKAMATNDIKHV
                     PYEITREKFKQAILDVETYHATQESQQGYKKIS"
     gene            10838..11386
                     /gene="aac(6')"
                     /locus_tag="SCJ86_002364"
     CDS             10838..11386
                     /gene="aac(6')"
                     /locus_tag="SCJ86_002364"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008265821.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminoglycoside N-acetyltransferase AAC(6')-Ii"
                     /protein_id="HEG1169885.1"
                     /translation="MIISEFDRNNPVLKDQLSDLLRLTWPEEYGDSSAEEVEEMMNPE
                     RIAVAAVDQDELVGFIGAIPQYGITGWELHPLVVESSRRKNQIGTRLVNYLEKEVASR
                     GGITIYLGTDDLDHGTTLSQTDLYEHTFDKVASIQNLREHPYEFYEKLGYKIVGVLPN
                     ANGWDKPDIWMAKTIIPRPNSQ"
BASE COUNT         3822 a         1947 c         2483 g         3261 t
ORIGIN      
        1 cgaaaaaaga agcattttta tatatgcttc ttttttcgta gaatcaataa atatggattt
       61 ggctaactat ggaagaatac tcgtattagt tttcagcgtt catactagtt gttacattgt
      121 ttgattggcc aaaagaattt ctgatatatt ttgtgctaaa taaagaaaaa gacgcttgaa
      181 ttgggaaata agaaaactac tctatcatta caaattttca taaactggca gatgttctaa
      241 gtaaacaagt atcttttcaa agtcggaaaa atccataatc aacgtagtgg tattgatatt
      301 tggatgaaag cctacggtat cttgacgatc aatagcagca tcgatcacga cttggatttt
      361 tttctctttg tcgtgcatca atgctaatgg agtcactgta cctgctggaa cgcctaggag
      421 gtcaggaagc tgttcttcag aaacaaaaga taagcgcttt tcttttaatt gttcagctag
      481 ttgttttaaa tcggcgtgct tttcatcagg caagatgaca agatagattt gttttccttt
      541 tcttgatttc aataatagat tttttacttg cggaccaggt agggaaaaag ggacattttt
      601 gactgacgta atcgcaggat gatcgacttt ttgaaaagag atacccagtt tttttagtag
      661 ttcataagct tcttgttcgg ttgcacgact catcgcgcat caccctcctt tttttattag
      721 tgtatcataa aaaagagttg acaaatgaaa aataatcatg taaattacac gtaaagttaa
      781 aacaaatgac atcgaaaaga agagtagccg ttaggtgttt ttagagagtc tctggttggt
      841 ggaaagaggc agcagttaac cgtgaaacac atcttggagt cgattttctg aagcgtatag
      901 taggaaaatc cggggagccc gttacagctg cggtcgttgc tagatgacca gataaggctg
      961 gcttttccgt gaggagtcgg taaaaaaagg tggaaccacg tgattaacgt cctttgatct
     1021 tcggatcaaa ggactttttg ttttcatctg aacggcaacg actgtgtgag gttttttctg
     1081 caaggaaaaa ataaacagag gtggaaccac gtataatacg tcctcttggc atttgccaag
     1141 aggatttttt tattttaaag gaggaaaaga agatggagta tttgattgaa gttatgccgc
     1201 aattattagt aggagcactc acaacattga aattattttt atttaccttg attggctcat
     1261 taccattagg gattgtttta gcattaggat tgtctttgca ttttaagccg ctacgctact
     1321 tgcttcaaac atatgtatgg gttatgcggg gaacaccgct gttgttgcaa ctgattttta
     1381 ttttttatgg gttgccgacg attgggatcg tttttgatcg tttcgaagca gcactcattg
     1441 cctttatctt aaactatgca gcttattttg cagagatctt tcgtggaggg atacaatcga
     1501 ttcctcaagg tcaatatgaa gcagcgcaag ttttgcaatt aagtcccttt caaacggtga
     1561 cgaaaatcgt catcccacaa gtgacaaaga ttgttttacc ttctgtaggg aatgaagtta
     1621 tcaacttggt caaagattct tccttgatct atatattagg tttaggtgac gtaatgcgtg
     1681 ctgggaaaat cgcgatggaa cgcgacgcga ctttactgcc tttgctaggc gtggcaatca
     1741 tctatcttac attgactggt attctaacaa tcataatgaa acaagttgaa ggaaaattaa
     1801 attattaccg ctaactgata ggaggaagaa atatgattga attgaagaaa gtgaacaaag
     1861 catttggtga aaaaaaaata atcaaagatt tgaatctaat cattccagat gggaaaacat
     1921 tagctatcgt aggtccttcc ggtggcggta aaaccacgct tttacgtatc ttggctggct
     1981 tagaaagttc tgacagcgga gaattttatt tagacggtgt accgttcaac ccattcgagc
     2041 ttaaaatgaa agaacaagtt gtaggagtcg tattccaaga cttccagcta tttccacatc
     2101 tgaatatttt tgaaaacatt accttagcac caaaaatggt attgaaacag acaaaagaag
     2161 aatacaccaa aaaagcagaa gaattagcgg cttcattagg gattagtgat ttattgaata
     2221 tctatcctta tcagctttct ggcggacaaa aacagcgggt agcgattgcc agagcattgg
     2281 ctatgaatcc acgggtattg gcttatgatg aaccaacaag tgctttagat cctgaattac
     2341 gccagcaagt cgagcgtttg atcctatcat tgaaagaaga tggggtaacc caaattattg
     2401 tcacccatga tttagtattt gcagaaaatg tcggtgatca attactaaaa gtttccccaa
     2461 ttcattaaga aaggagaaga tagagatgaa aaaaaccagt tttctattcg ctttgatcgc
     2521 aggtcttctt ctttttgcag gctgttcgaa caaaaaaact tcagcagata attgggcgac
     2581 tatcaacgaa gagaaaaaaa tcgttatagg tttagacgat acatttgttc ctatgggatt
     2641 tagagataaa gacgggaagc tcacaggatt tgatattgat ttagccaaag cagtctttga
     2701 acaatatggg attacggtag actttcagcc cattgactgg tcaatgaagg aatttgaact
     2761 gaacaatgga acgatcgatt tgatatggaa cggctattcc aaaacgccgg caagagaaaa
     2821 gaaagtgcaa tttacaaaac cttatatgga aaacgatcaa gtgctgatca cacctaaaga
     2881 atcaggaatc acaaactttg gacaaatgaa taataagaga ttaggtgcgc aaaatgggtc
     2941 atctggttat gatgtattta cgaaacaacc agaagtatta aaagagatag tcaaaaacca
     3001 agatgctgtt ttgtatgata gttttaatga agcattgatc gatctgaaaa gcagacgggt
     3061 cgatggtctg ctgatggata aagtatacgc agactattat ttgaaacaaa gaaacgaact
     3121 agaagacttc aacataacaa aaggttctta tgagagtgag gattttgcag ttggtgcacg
     3181 caagtcagat agtacattag tgaaaaaaat caatgaagcc ctagatcaac tagagaaaac
     3241 aggtgaattc caaaaaatat ccgataaatg gtttggcgaa gatgttacgc cgagaaatta
     3301 acaaataggg gactgtgaca aaagacgtgt cacagtccct ttttccgaat aaacagtgtt
     3361 ctcctgccaa ctccttgtga ctcggagctg aatggcaggc tcacaacctc ttggtaaacg
     3421 gtgttcaaaa gccagttttt cggtattaag ccaaatttat gcaaaaatgt agaaagccat
     3481 tttcacaaaa tttttcttac tactcgaaac taagcgactt tttcacaacc tcctttacat
     3541 aattttattt tgttgtggaa ggggtttcgt gttattctaa aagaggaacg attgaaggtt
     3601 ataggaaagg aagagatgaa tggaacacaa agaacttacc tcatttccaa aagattttct
     3661 gtggggctct gcttctgcag cgtatcaagt agaaggagct tggcaagaag atgggaaagg
     3721 agaatcagtc tgggatcgat ttgtacggat acctggaaag acatttaaag gaacgaacgg
     3781 tgatttggca gtagaccact atcatcgtta taaagaagat attgctttaa tgaaagaaca
     3841 aggtctgaaa gcttaccgct tctctgtagc ttggacacgg atttttccta atggacgagg
     3901 agaagtcaat caagctgggc tcgcttttta tgagagattg atcgacgaat tgattgaaaa
     3961 cgatattgaa cctatgttga ctttatatca ttgggatctt ccacaagcat tgcaagatga
     4021 atataatggt tgggaatcac gtcagatcat tgaagatttc actcattatg cagaaacatt
     4081 atttgaggca ttccgcggga aagtccatta ctggattagt ttgaatgaac aaaatatctt
     4141 cacttcatta ggttatttgt tagcagcaca tccgccaggt gtgacagacc cgaaacgaat
     4201 gtatgaagtt aaccatattg ctaatttagc aaatgcatct gtcatcaata aatttcacga
     4261 gttagaaatg cctggaaaaa tcggaccaag ctttgcgtat acgccaaatt atccaattga
     4321 cagtaatcct gaaaatgttc tggctgctga aaatgcagaa gacctgatgg ccaattattg
     4381 gatggatgtc tatatgtggg gaaaatatcc gattgctgcc atgaggttct tagaagaaaa
     4441 aggatgggca ccaacgattg aagctggcga tgcagaattg ctagaatctg ctaaaccaga
     4501 ttttcttggg atcaattact atcaaacggc gacgaatgca ttcaatccat taaacggagt
     4561 tggtgctgga aaaatgaata cgacaggaaa aaaaggttca tcagaagaaa ctggagttcc
     4621 tggaatgtat aaaaaagttg agaatccatt tgtcgaacga acaaattggg actgggagat
     4681 tgacccagaa ggattaagga tcggactgcg tcgtatcaca agccgttacc gcatgccggt
     4741 actgattacg gaaaatggct taggagagta tgacaagttg acagaagata aacaaatcca
     4801 tgacgactat cgaatcaatt acttacaaag ccacgtcaaa gccatcaaag aagcaatttc
     4861 ggatggagca gaagtcctag gctattgcac ctggtcttat acagatttgc ttagctggct
     4921 gaacggttat caaaagcgat atggatttgt atatgttgat caagacgaaa cacaagaagg
     4981 atctttggaa cgatataaga aagacagtta ttactggtac caaaaactga ttgaagaaaa
     5041 tggagagaac ctttagttca catacttaaa acaagaaggt acggctggat atggccgtgc
     5101 cttttcttga taaaatcatt tagaaattaa gaaaaactgt aaaataagct gaaaaatgaa
     5161 agaaagcgtc agttctttca aacaaaactt tagttccctt actttttttt gtataatgaa
     5221 acttgatgaa atgagggata ataaatggca caaaagaaac ggcctactaa aaaaaggaaa
     5281 aagacgaaaa aaacgaaaaa gcaaaaacag cagcaggaac aaatgctgac gattggtgtt
     5341 gggtttgcct tgattttatt tgctgtcttc ggctttttaa aattaggttt tttaggaata
     5401 ttgattgcaa acggctttcg gatcattgca ggaaatactt accaaatcct ctgtctttta
     5461 ttagccgtat taggattttg gattgtcatt aagaatacag agttttcaat tggtaaaaac
     5521 cgacgctggt ttggcggcat actcttttat ttcggtatct tattattgtt acatgcccat
     5581 ttatttggca aactccatac tggggaacca aatatcatgg ggacgacatg ggatttgtta
     5641 gccagtgata taaaacaaag tcaagtagac aataacgttg gtggtgggat gattggagca
     5701 atcctttatc attttactta cttcttgatt gcacagcctg gaagttattt agcagcagtg
     5761 ctattactgg caggaggagc attcctgttc agtaatctgg aaggctatca gctgttaaat
     5821 ggtatccaat caattggaga acgagtacag gaattattgg aaggtgatcc tgccaagcaa
     5881 gctcgaaagc aagcggcaaa ggaagagaga atgaaacagc gcgcagaagc gaaagaagca
     5941 aggcgcttag ctgcaaaaga agctgcagaa aaagaagctg cagaatatga gaagaaaaaa
     6001 gcggtgagcc agcaaaggga aaatactgct gatgaatggc aggaaccaga aaatacagag
     6061 ccggagcagc taagttttgt tccaatcgac agttttcaag aaaatatcca tcctgcgaat
     6121 ctagagaaac cagtaccaga tacaccaaaa cagaccaata cagcagaagg tttttcagat
     6181 gagttgccag aggatgacgg tacctcatta gaatttgaga ttgaagcaga acaggaaaat
     6241 caagactatg aattaccgac agttgatttg cttgattcta taccaactgt ggatcaaagt
     6301 gatgagtata aaaaaattga aaaaaatatc ggcgtattag aacaaacttt ccaaagtttc
     6361 ggtgtcgatg ccaaggtcgt caaagccagc ttaggaccat ccgtcacaaa attcgaagtc
     6421 cagccggctg taggggtaaa agtaagtaaa attgtgaact taacagatga tatcgcgctt
     6481 gctttagcgg cgaaagatgt ccggatggaa gcaccgattc caggtaaatc actgattggt
     6541 attgaagtac caaatggaaa gatcagtatg gtttccttta gagaaatcat cgaagcacaa
     6601 ccaaaccacc cagataaatt actagaagta cctttaggac gtgatgtttc tggacgggta
     6661 caaacggctg atctttcgaa gatgcctcac ttactcgtgg ctggttcgac tggtagtggg
     6721 aagtcagtag caattaatgg gattatcaca agtatattaa tgcgtgctaa gccccatgaa
     6781 gtaaaactga tgatgatcga tccgaaaatg gttgaattga atatgtacaa cggcatacct
     6841 catctgttga cgccggtcgt gacgaaccct agaaaagcag cgcaagcctt acaaaaagta
     6901 gtacaagaaa tggaagaacg atatgaaaaa ttcgcggcaa ccggagtaag aaatatttct
     6961 ggttacaatg aatttgttca acagaaaaac ttggaaaacg gaacgaaaca tccaacctta
     7021 ccattcatcg tagtgatcgt agatgaatta gctgatttga tgatggtagc cagcaatgaa
     7081 gttgaagatg cgattattcg tttagcgcaa atggctcggg cagcggggat tcatatgatc
     7141 ctagctactc aacgtccaag tgtggatgtt atcacaggaa tcatcaaagc aaatgtacct
     7201 tccagaatgg catttgcggt atcaagcggt acagattctc ggacgatcat tgattctaat
     7261 ggggcagaaa agctcttagg gcgaggagat atgctattct tgccaatggg tgagaataaa
     7321 ccgatccgtg tgcaaggagc attcatttct gaccacgaag tagaacgggt cgttcaattt
     7381 gttacagatc agcaagaagc tcattatgaa gagaaaatga tgccgacaga tgaagttgaa
     7441 acgacaggtg ctccagaaca gccacaagat gagctgtttg aagaagcaaa agcacttgtc
     7501 gttgaaatgc aaacggcaag tatttccctt ctccaaagaa gatttcggat cggttacaac
     7561 cgagcagcac gtttagtaga tgaactggaa gctcatggag ttgttggccc gtcagaagga
     7621 agcaagcctc ggaaagtatt cattgagcag gaagaaacat cagaaacgtt gccggacgat
     7681 aatgacatgt aaaacttgtg tgatgaaggg ttgaaaaacg aataaccaat aaaaagacaa
     7741 ggcatgatga taccaaaaga aaaaatggtt tttttccatc ttttctaata cggtatgatg
     7801 tcttgtcttt ttagtttttg tgagattaaa gttctattcg atttatttgt gaagtattgc
     7861 attaaggaaa tgaatcatgt atagtatgta gcgaaacttt gagaaaaaga gggaaaatac
     7921 tttggaaaaa cagtccattt atggcttgac gaatgaagaa ttaatcaatt ggtttataga
     7981 aaacggcgag aaaaaattcc gagctgccca agtttgggaa tggttgtatc aaaaacgtgt
     8041 aagtaatttc acagaaatga cgaatctatc gaaacaattg atcgaaaaat tatcggagca
     8101 ttttatcatc aatcctctaa aacagatggt cgtccaagaa gcttcggatg gtacagtaaa
     8161 atatttgttt gaactccctg ataaaaacat gattgagact gtactgatga gacaagaata
     8221 tggcttgtca gtttgtgtga cgacacaagt aggatgtaat atcggctgta cgttttgtgc
     8281 aagcgggctg ctcaaaaaga accgtgatct gactgctgga gaaattgttg cacagatcat
     8341 gatggtccaa cattattttg atgagcgcaa tcttggcgaa cgcgtttcac atgtcgttgt
     8401 aatggggatc ggtgagccat ttgataatta cgacaatgta atggactttt tgcatatcat
     8461 caatgatgcc aaaggattgg caattggtgc tcggcacatt acagtttcca ctagtggatt
     8521 agcacacaaa atcaaggaat tcgctaataa cggcttgcaa gtcaatctgg ctatttcctt
     8581 gcacgcgccg aataatgagg tgcgaacatc gatcatgcgg atcaaccgaa gtttcccaat
     8641 tgagaaattg atggaagctg tggatgagta tttagagaaa acaaaccgtc gaatcacgtt
     8701 tgaatatatc atgttgaatc aagtaaatga tagaccagag catgcgcaac aattagcaga
     8761 tctattaaaa gataagaaaa aattagcata tgtcaacttg attccatata atccagtcag
     8821 tgaacatgat caatattctc gcagtccaaa agctgatgta ttgagatttt atgatgtatt
     8881 gaagaaaaac ggcgtaaact gtgtgatacg taaagaacat ggtacagata ttgatgcagc
     8941 ttgcggacaa ttgcgaagca agcaaatgaa aaaaacagtt gccaaataat cgtatcctac
     9001 tcgttttttc aattaccata aaaggagcag ttttcttaat taaggaactg ttcaacttaa
     9061 ataagagaaa agattctata gcgaaagcta taaagctttt tttgacaaga aaaaccctca
     9121 tcaagcaaag aaatgtcctc aatttgatga cagcctgctt gatgggggtt ttagcaatta
     9181 tttaactgat tttcttatat ccttgctggc tttcttgtgt agcatgataa gtttctacgt
     9241 caagaatggc ctgtttaaat ttttctcggg tgatttcata aggcacatgt ttaatatcat
     9301 ttgttgccat ggctttatct aagattgctt ctaaatcggt cgtgacattg agattcatgt
     9361 cggttaacga tactggaagt tggttctttt tcataaacac atagatttta tcaaacaaat
     9421 cgaactgctg atccatcatc aataaggtca gtacaccata actgacagat gctccgtgaa
     9481 ggtatccctc cattttttct agaacagtcg taccgtaata gaatgagtgt gccatagcag
     9541 aattatagtt attgttaact aaaacagaaa ccaatccggt agtaacaatg atatccgaaa
     9601 cgacttgttc aattgcatat gagggacgat cgtcggcact atctttcaat gcttgcgtac
     9661 cataagtcaa gagacgatca atgcacattt ttccgatttg gattcctaat ctatccgtgt
     9721 agcctagatc atcgccacga gcagaaaaag tagattcgac ttctttggat aaagcatcgc
     9781 caatacctgc ccataagtat ttatcaggtg cttcggcaat aattgctgta tcaatgaagg
     9841 tgtgtacagg tggttttgaa ggaaagaaga gtccgtccat tacgccttct tcgttataga
     9901 tgacacatac cgcagtgacg ggcgaacagt tcgaaccaat agtgggaaag gtgaagactg
     9961 gtttagctag tttttcacca atgactttta cagtatcgca agcacggcca ccaccgactg
    10021 caaacaggac gtctgctttt tggacacttt catgctggat caactgttca atatttccat
    10081 aagttgaatt acccccgtac cagagactgg cgacaacctc tatttcctgt gtcaaagcta
    10141 cttttatttt gttttctgcc tttgaaagtg cagtcttccc tccaatcagc actacttttg
    10201 tcccatattc tttgacgatt ccatttgctt tttcatacgc atctgttcca atggtataat
    10261 ttggtaagaa aacactttga ctcatattca tttccttctt tcttcgttga aacgggttaa
    10321 aattttaaag agtgatcgat ctgactgcgg ctgtagatgg agtagaggtg aacctaagac
    10381 aggataagtg atgaataagg tggcatgaac accttgaaat catcaataag tatcctaaaa
    10441 acgaacaagt acaaaaacat tgaaaacgac aataatgaat ttttgttcct cttagtattt
    10501 ctaccgattc cctttgcttt tttgtaatta tctaaaaatt ctaaaaatta agattattat
    10561 atgaaaacag gttttatttg tcaattcata attggttttt tttaaagcaa aagataatct
    10621 atatcatgat gaacaaacag agttttttcc tccaattgat ggctttcctg atctttccta
    10681 ttttgaataa agtgtttctg tataattttt ttattttata agtaagataa ataatgaaag
    10741 aatagagata ctgattggta gctaaagatt tttgagcgag aacatttcgt tttttcaaaa
    10801 ggactgttag aataagcaag aacaaggggg aagacgtatg ataatcagtg aatttgaccg
    10861 taataatcca gtattgaaag atcagctttc tgatttactg agactgactt ggccggaaga
    10921 atatggagac agctcggcag aagaagtaga agaaatgatg aatccagaac gaatcgcggt
    10981 agcagcggta gaccaagatg agttagtagg atttattggt gcaatccctc aatacggtat
    11041 cacaggttgg gaattgcatc cattagttgt agaaagctcc cgacgaaaga accaaatagg
    11101 tactcgatta gtcaattact tagaaaaaga agtagcttcc agaggaggaa tcacgattta
    11161 tttaggtacg gatgatttag accatggaac aacgttaagt caaacggacc tgtatgagca
    11221 tacatttgat aaagtggctt ccatccagaa ccttcgtgaa catccgtatg aattctatga
    11281 aaaattaggt tataaaatcg taggtgtctt accaaatgca aatggctggg acaaaccgga
    11341 tatttggatg gcaaaaacga ttattcctcg accaaattct caataataaa aagaggttgt
    11401 gacagaagta atcagcttca agaaataaga aggaatttac gaaaattact tcttgaattt
    11461 ttgtgaaatt ccaacttatt tccgaagaag ctacttttgg aacaccgtgt acc
//
LOCUS       DARBET010000081        10924 bp    DNA     linear   BCT 07-NOV-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4027
            SAMEA114569841-rid20112183.denovo.083, whole genome shotgun
            sequence.
ACCESSION   DARBET010000081 DARBET010000000
VERSION     DARBET010000081.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA114569841
            Sequence Read Archive: ERR12203666
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 10924)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10924)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001972618.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001972618.1
            Genome Coverage       :: 132x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/07/2023 13:18:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,041
            CDSs (total)                      :: 2,975
            Genes (coding)                    :: 2,908
            CDSs (with protein)               :: 2,908
            Genes (RNA)                       :: 66
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 67
            CDSs (without protein)            :: 67
            Pseudo Genes (ambiguous residues) :: 0 of 67
            Pseudo Genes (frameshifted)       :: 26 of 67
            Pseudo Genes (incomplete)         :: 28 of 67
            Pseudo Genes (internal stop)      :: 20 of 67
            Pseudo Genes (multiple problems)  :: 6 of 67
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10924
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA114569841-rid20112183.denovo.083"
                     /isolate="VRE4027"
                     /db_xref="taxon:1352"
                     /country="Denmark"
     gene            complement(<1..281)
                     /locus_tag="SCJ86_002365"
     CDS             complement(<1..281)
                     /locus_tag="SCJ86_002365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310346.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEG1169886.1"
                     /translation="MKVVQTKDTMSNIYLDAVRIRHQVFVVEQGVPLSREIDKDEAHC
                     IHFVLYSDKKEPQGTVRLLPLENGKMKLQRMAILSEYRHQGLGKILIEEA"
     gene            423..1541
                     /locus_tag="SCJ86_002366"
     CDS             423..1541
                     /locus_tag="SCJ86_002366"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341268.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter"
                     /protein_id="HEG1169887.1"
                     /translation="MFEKLRQSKLMFWSLELLIIAALIMISSEINFIFEPIGTFFSTL
                     FAPVLIAGFLYYLLNPIVKLLMKAKIKRIWAVAIVLLLLVAAIIWILLSVIPSLVQQI
                     SSLASNMPDFIKQVESWLKEVAELPLFKEVDINKYFEQLDISYGTIIQQFLSGVSNSL
                     GSIVGTIASATVVIITAPFILFYMLKDGEKLVPNIQQFFPEKRRNQIVELLGQLNKTL
                     ANYISGQAIECLFVGTFTFLGYMVIGVDYAFLFGVIAGLTNLIPYLGPYLGLAPAFLV
                     TVFNDPVKALLCCVVVLIVQQLDGNIIYPNVIGKSLKIHPLTIIIVLLVAGNIAGLLG
                     IFLGVPFYAICKTIISYIIKIVKEDKQNENKKKIATTS"
     gene            1669..3045
                     /locus_tag="SCJ86_002367"
     CDS             1669..3045
                     /locus_tag="SCJ86_002367"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016610209.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HlyC/CorC family transporter"
                     /protein_id="HEG1169888.1"
                     /translation="MNADPESQSLLAQILLLIVLTLINAFLAASEIAVVSINKNRIEQ
                     KADEGDVKSQKLLKILQNPNNFLSTIQVGITLVNILSGASLANTLSERLAPVLGGGTA
                     AKNIANIIVLAILTYVSIVFGELYPKRIALNKSEEVAKFTSGLIRLIGVVAKPFVWLL
                     SASTSLLARITPMTFDDEDSKMTRDEMRYMLENEGVLNNEELEMLQGVFSLDTKVARE
                     VMVPRTDAFMIDINDTVEENVNEVLSENYSRIPVYNEDKDKVVGILHTKNLLKAAHKF
                     GFDNLDIKKIMQEPLFVPETVFIDDLLYEMKKTQNQMAILLDEYGGVVGLATLEDLLE
                     EIVGEIDDESDEVENLYEKIDEHEYIIQGRMLIDEFNEAFDSDLHMSDVDTMAGYLIT
                     ALGMIPDEGEKLSFDVDNITLVSEEMEGSRILKIRVIFHDPEETEAEPEEERRYFRKE
                     FEDDEPRR"
     gene            3446..5026
                     /locus_tag="SCJ86_002368"
     CDS             3446..5026
                     /locus_tag="SCJ86_002368"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290823.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor 3"
                     /protein_id="HEG1169889.1"
                     /translation="MNNPKLKQQVDNRRTFAIISHPDAGKTTITEQLLLFGGAIRQAG
                     TVKGKKTGNFAKSDWMEIEKQRGISVTSSVMQFDYQGKRVNILDTPGHEDFSEDTYRT
                     LMAVDSAVMVIDSAKGIEAQTKKLFQVVKKRGIPIFTFINKLDRDGREPLELLEELEE
                     LLDIESYPMNWPIGMGKGLEGLYDIYHNRVEFYRPENYQDERLVELDEDGLLPENHPL
                     TENSLYEQVLEEVELIKEAGDAFNQEKIARGDQTPVFFGSALTNFGVQTFLETFVELA
                     PAPYGHKTQDDQMVSPYEEEFSGFVFKIQANMNPAHRDRIAFVRICSGTFERGMDVWL
                     ERTNKKLKLSNVTQFMADSRENVEKAVAGDIIGVYDTGNYQIGDTLFEGKLKVAYEEL
                     PSFTPELFMKVTAKNVMKQKSFHKGIYQLVQEGAIQLYKTYLTEEYIIGAVGQLQFEV
                     FQYRMLNEYNAEVIMSPMGHKIARWIDPEDLDEKMSSSRNILARDRFDQPLFLFENQF
                     AERWFADKYPNVKLKSLM"
     gene            5224..5447
                     /locus_tag="SCJ86_002369"
                     /pseudo
     CDS             5224..5447
                     /locus_tag="SCJ86_002369"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287661.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar motor switch protein"
     gene            complement(5652..5972)
                     /locus_tag="SCJ86_002370"
     CDS             complement(5652..5972)
                     /locus_tag="SCJ86_002370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326480.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1827 family protein"
                     /protein_id="HEG1169890.1"
                     /translation="MKLVNVTNSHSRLVLNQLENTDAHLVKVYTAGNTTIVYTEAPEH
                     NEILLINDKRKIQPKEIEDAKNYFLDKIKDAVYHEDEIKVIELAGLVEISIPKHELGA
                     TLAV"
     gene            complement(6120..8354)
                     /locus_tag="SCJ86_002371"
     CDS             complement(6120..8354)
                     /locus_tag="SCJ86_002371"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287598.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HEG1169891.1"
                     /translation="MLCQNCGKNEATIHLYATVNGQRTQLDYCQSCYQKIKNQQNGGL
                     TNMAQNDPFGFGSLDDLFRSMSRQMQQQGNYEQTPPTQFGGNNNFNGGQPPQGNTEGL
                     LGEYGINITEQARQGDIDPVIGRDDEIKRVIEILNRRTKNNPVLIGEPGVGKTAVVEG
                     LAQKIVDGDVPQKLMDKEVIRLDVVSLVQGTGIRGQFEERMQKLIEEIRQAENVILFI
                     DEVHEIVGAGSAGDGNMDAGNILKPALARGELQMVGATTLNEYRIIEKDAALERRMQP
                     VRVDEPTVEETISILKGLQKRYEDYHHVKYTDEAIEAAATLSNRYVQDRFLPDKAIDL
                     LDETGSKKNLTIQIVDPKTIEKKLQEAEEQKVLASREEDFEKAAYYRDQINKLQKMKE
                     RQLTEEETPVITEKDMEKIVEQRTGIPVGELKEKEQTQLKNLADDLKAHVIGQDNAVD
                     RVAKAIRRNRVGLNKQNRPIGSFLFVGPTGVGKTELAKQLAYELFGSQDSMIRFDMSE
                     YMEKHSVSKLIGSPPGYVGYEEAGQLTEKVRRNPYSLVLLDEVEKAHPDVLHMFLQIL
                     DDGRLTDAQGRTVSFKDTIIIMTSNAGTGKVEANVGFGAAREGVTRSVLNQLNNYFTP
                     EFLNRFDGIIEFSALSKENLMTIVTLMLDDVNQMLAAQQLHIEVPTNVKEKLVDLGYD
                     PSMGARPLRRTIQEQIEDGIAEFYLDHPSIHELKAKLDKDGKIIVTSKPERLAKESAE
                     ETAE"
     gene            8833..9099
                     /locus_tag="SCJ86_002372"
     CDS             8833..9099
                     /locus_tag="SCJ86_002372"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010773230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphocarrier protein HPr"
                     /protein_id="HEG1169892.1"
                     /translation="MEKKEFHVVAETGIHARPATLLVQTASKFNSDVNLEYKGKSVNL
                     KSIMGVMSLGVGQGSDVTITAEGADEADAMAAIVETMKKEGLSE"
     gene            9099..10826
                     /gene="ptsP"
                     /locus_tag="SCJ86_002373"
     CDS             9099..10826
                     /gene="ptsP"
                     /locus_tag="SCJ86_002373"
                     /EC_number="2.7.3.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005876303.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate--protein phosphotransferase"
                     /protein_id="HEG1169893.1"
                     /translation="MVEMLKGIAASDGVAVAKAYLLVQPDLTFSKATVEDTAAEEARL
                     DAALAKSTEELQQIREKAAQSLGEAEAQVFDAHLMVLSDPEMVGQIKQNIKDNSVNAE
                     SALKEVTDMYIGMFEAMEDNAYMQERAADIRDVAKRILAHLLGVTLPNPSMINEEVVV
                     VAHDLTPSDTAQLDRNFVKAFVTDIGGRTSHSAIMARSLEIPAIVGTKEITAKVKEGD
                     ILAVNGIEGDVIIDPTDEQKAEFEKAGADYAAQKAEWEKLKNAETVTADGKHFELAAN
                     IGTPKDLVGVHNNGGEAVGLYRTEFLYMDSPDFPTEDDQYEAYKAVLEGMEGKPVVVR
                     TMDIGGDKELPYLQLPHEMNPFLGYRALRISLSEQGDEMFRTQMRALLRASVHGNLRI
                     MFPMVATLKEFRAAKAIFEEEKQKLISEGKEVSDTIQVGIMIEIPAAAVLADKFAKEV
                     DFFSVGTNDLIQYTMAADRMNERVSYLYQPYNPSILRLIKNVIDAAHAEGKWAGMCGE
                     MAGDQTAVPLLVGMGLDEFSMSATSILKTRSLMKRLDTAKMAELADRALKECDMMEEV
                     VELVHEYVK"
BASE COUNT         3480 a         1911 c         2215 g         3318 t
ORIGIN      
        1 gcttcttcaa tcaatatttt tcctaaccct tggtgtctgt attcagaaag aatcgccatc
       61 cgctgtaatt tcatttttcc gttttctaaa ggcaaaagac gtacagttcc ttgcggttct
      121 tttttatctg agtacaggac gaagtggata caatgggctt catctttgtc tatttctcga
      181 gacaaaggca caccctgttc gactacaaaa acttgatgcc tgatccgtac agcatctaag
      241 taaatattgc tcattgtatc ttttgtttgt acaactttca tggtctagcc accttccttt
      301 cgtatagtat actggaaaat aaaacaaata aaaagtatgg caagttttca aaaataaaaa
      361 aagaataaaa gaatttttaa cgaatataat atatactagt aagaaagaaa ggaggaaatc
      421 tattgtttga aaaacttaga cagtctaaac taatgttttg gtcgcttgaa ttattgatca
      481 tagctgcatt gatcatgatt tcatcagaaa taaactttat tttcgaaccg atcgggacat
      541 ttttttccac gttatttgca cctgtactga ttgcaggctt tctttactat ttattgaatc
      601 ctattgtcaa gctgttgatg aaagcaaaaa tcaaaagaat atgggctgtc gcaattgtgc
      661 tgctcctttt agttgcagca attatttgga ttttacttag tgtcatccca agtctagtac
      721 agcaaatttc ctcattggca tctaatatgc ctgatttcat caaacaggta gaaagctggt
      781 taaaagaggt agccgaattg cctttgttca aggaagtaga tatcaataaa tattttgagc
      841 aattagacat ttcttacggg acaatcattc agcaattcct tagtggagta tccaatagtt
      901 taggttcgat cgttggaacg atcgcttcgg caaccgtagt aatcattaca gcaccattca
      961 tcttgttcta tatgttgaaa gacggagaaa aactagtgcc aaacatccaa cagtttttcc
     1021 cagaaaaaag aagaaaccaa attgtggagt tattggggca actgaataaa acattggcta
     1081 attacattag cggacaagca atcgaatgtt tattcgttgg taccttcaca tttttaggat
     1141 atatggtaat tggtgtggat tatgctttct tatttggggt cattgcggga ttgacaaacc
     1201 taattccata tttaggacct tatctaggac tagcacctgc atttctagta acagtcttca
     1261 atgatccggt aaaagcatta ctttgttgtg tggttgtgct aatcgtccaa cagttggatg
     1321 ggaatatcat ttatcctaat gtaattggga aatcactgaa aatccatcca ttaacgatca
     1381 ttatcgttct tctagtagct ggaaatattg ccggattgct tggtatcttc ttaggtgttc
     1441 ctttttacgc aatctgcaag acgatcattt cctatataat caaaatcgtc aaagaagaca
     1501 agcaaaatga aaacaagaag aaaattgcta cgacctcgta ggtagtttta gtacacttgt
     1561 ttttattgca aagaaaattg ctttgtgata ccattttaac gtggctatag tgccacgttt
     1621 ttttctatcg taaactaaaa attaatttta taaggagaga atgcgattat gaatgctgac
     1681 cctgagagtc agtcgctgtt agcgcagatt ttattattga tcgttttgac attgatcaat
     1741 gcttttttag cagcttccga aattgctgtt gtttcgatca ataagaatcg aatcgagcaa
     1801 aaagctgatg aaggagatgt gaaatctcaa aagcttttaa aaatattgca gaatccaaat
     1861 aatttcctat cgacaatcca agtaggaatc actttggtca acattttgtc tggtgcttca
     1921 ctagctaata ctttatcgga acggttagct ccggtattag gtggcggcac agcggcaaaa
     1981 aatattgcaa atatcattgt gctggctatt ttgacctatg tatcgattgt ttttggagaa
     2041 ttgtacccta aacgtattgc tttaaataaa tcagaagaag tcgcaaagtt cacttcaggg
     2101 ctgatacgct tgattggtgt agtagctaag cccttcgtct ggctgctttc tgcttccaca
     2161 agtttattgg cgcgtatcac gccgatgact tttgatgatg aagattcaaa aatgaccaga
     2221 gatgaaatgc gttatatgct tgaaaacgaa ggcgtattga ataatgaaga attagaaatg
     2281 ctccaagggg ttttttctct tgatacgaaa gttgcccgag aagtcatggt cccacgaacg
     2341 gatgcattta tgattgatat caacgacact gtggaagaaa atgtgaatga agttttatcc
     2401 gaaaactatt caagaatccc tgtttataac gaagacaaag ataaagtagt cggaatcctt
     2461 catacaaaga atttactaaa agctgcccat aaattcggct ttgataattt agatatcaag
     2521 aaaatcatgc aggagccttt attcgttcct gaaacggtct ttattgatga tctgttatat
     2581 gaaatgaaaa agacgcagaa tcagatggct attttattag atgaatatgg cggcgtcgtt
     2641 gggttagcaa cactagaaga ccttttagaa gaaatcgtcg gagaaatcga tgatgaatca
     2701 gatgaagttg agaatcttta tgaaaaaatc gatgaacacg aatatatcat ccaaggacgt
     2761 atgctgattg atgagttcaa tgaagctttt gacagtgatc tgcatatgag tgatgtagat
     2821 acgatggctg gttatttgat tacagcatta ggcatgattc cggatgaagg cgaaaagtta
     2881 tcgtttgatg tcgataatat tacgctggtt tctgaagaaa tggaaggttc tcgaatcctt
     2941 aaaatccgtg tcatttttca tgatccggaa gaaacagaag cggaacctga agaagaacga
     3001 agatacttca gaaaagaatt cgaagatgac gagccaagaa gataattgga aacttggtta
     3061 tgaaagacgt gttgcttgaa taaacaagca acacgtcttt tcttttagtc agctgacaaa
     3121 aaaatatttg actcttatgg tttggtggct ttttcattcg ctgaatccat gctatactga
     3181 ttcagttgta gtagttgaca aaggttgtga aaaagctatc ctgcattaag taataagagc
     3241 agccaagcaa aaacagcttt tcgtattttt ggcttgggtg ggcttattgc tgataggctg
     3301 tctctagaac gccgtttact cggaaaaaga gactgctaca cggcttttgt cacagcccta
     3361 tatgaaattg tctttcttta agagatcgga caatctgtgc ctagagaaaa tataaataat
     3421 tgaatggaag aaatggagtt aggttatgaa taatccaaaa ttaaagcaac aagtggacaa
     3481 tcgcagaact tttgcgatca tttcccatcc ggatgctggg aaaacaacga ttacagaaca
     3541 attactattg tttggtgggg ctatccgtca agccgggacc gtaaaaggca aaaaaacagg
     3601 taattttgcc aaatcagact ggatggaaat cgagaaacaa cgtgggattt ctgttactag
     3661 ttctgttatg cagtttgatt atcaagggaa acgagtaaat atcttggata caccaggaca
     3721 tgaagatttt tctgaagata cttatcgtac tttgatggcc gtggatagtg cggtcatggt
     3781 aatcgatagt gcaaaaggga tcgaagcaca gacaaagaaa ttattccaag ttgtaaaaaa
     3841 acgtggcatc ccgatattta cttttatcaa caaactagat cgtgatggtc gtgaaccatt
     3901 ggaattgtta gaagaattag aagagctatt agatatcgaa tcttatccga tgaattggcc
     3961 aatcggtatg ggtaaagggc tagaaggtct ttatgatatc taccataatc gtgtagagtt
     4021 ttatcgtccg gaaaattatc aggatgaacg attagtagaa ttggatgaag atggactgct
     4081 tcccgaaaat catcctctaa ctgaaaattc cttatatgaa caagtcttag aagaagtaga
     4141 actaatcaaa gaggctggag atgcatttaa tcaagaaaaa attgctcgtg gcgatcagac
     4201 accagtattt tttggttctg cattgactaa cttcggtgta cagacttttt tagagacttt
     4261 cgttgaattg gcaccagctc cttatggaca caaaacacaa gatgatcaga tggtaagtcc
     4321 ttatgaggaa gaattttcag gtttcgtatt caagatccaa gcaaatatga atccagccca
     4381 tcgtgaccga atcgcttttg tccgaatctg ttctggaaca ttcgaacgag gaatggatgt
     4441 ctggttggaa cgtacaaata aaaaactgaa attaagcaat gtcacacaat ttatggcaga
     4501 ttcgagagaa aacgtcgaaa aagcagtagc aggcgatatc atcggtgtat atgatacagg
     4561 caattatcag attggggata cattgttcga aggaaaacta aaagttgctt atgaagaact
     4621 cccttcattt actccagagc tgtttatgaa agtgactgca aaaaatgtca tgaaacaaaa
     4681 atccttccac aaaggaatct atcaattagt tcaagaagga gcgattcagc tttataaaac
     4741 atatctaacg gaagaatata tcattggagc tgttggtcag ctacaattcg aagtattcca
     4801 atatcgtatg ttaaatgagt ataacgccga agtaatcatg tctccaatgg gacacaaaat
     4861 cgctcgctgg atcgatccag aagatttgga tgagaagatg agttcaagtc gcaatatctt
     4921 ggctcgtgac cgtttcgacc aaccgttgtt cttattcgag aaccaatttg cagaacgctg
     4981 gtttgcggat aaatatccca atgtaaaatt aaaaagtttg atgtaaagaa gtcttgacac
     5041 aagctgccct gcatcaagta ataagaatag ccaaacaaaa acagcctctc atattttgtt
     5101 tagagccgtg acggattttc cgtgacggct ctttttacta tattaaaatc atagaaatgt
     5161 ctaatcaaaa aaatataaaa attattttgc gtacatatga ttaaagaaag gagagtgtga
     5221 cacatggaaa cagaatggca aagacgtgaa agaaaaaaac agaaaaagaa gagatgttac
     5281 gattatgtcg attattagat gtaaaagact gggacaaaaa cgagcaaatt gcacaaaaag
     5341 tacgatcaat catccataaa gaggaaaaag ttaaaaataa agacacgcgt agaactgaaa
     5401 atgaaaagag aaaagtacaa aagaaaagag aaaaatcaaa aagctagaaa gaaaaagagg
     5461 ctgagacaga agcgtttaac tccaagaaat aagctggaat tcacaaaaat ttgaaaagca
     5521 attttcgtga atttcagctt atttccgaag gagttgcttc tgctttcgcc gtttatacgt
     5581 gtttagagcg tgagacaaaa gtgttcttta cttttgtccc acgctcttta attattatgt
     5641 tcaaaatttg tttacacggc taatgtagcg cctaattcat gttttggaat cgagatttca
     5701 acaaggcctg ccaattcgat tactttgatt tcatcctcgt ggtaaacggc gtctttgatt
     5761 ttatctaaga aataattttt agcatcttcg atttctttcg gctggatttt acgtttgtca
     5821 ttgatcagca gaatttcatt atgttcaggg gcttcagtgt agacaatagt ggtatttccg
     5881 gctgtgtaga ccttgactag atgggcatca gtgttttcaa gctggtttaa tacaagacgg
     5941 gaatggctgt ttgttacatt taccaatttc atggcgctca cctcttttat gtatttttaa
     6001 ttgcttgttt ctcttattat aatttctttt ttataaaatg aacagtattt tgacttttct
     6061 gaaaaaagaa tgagacggca tttctcaaag aaataccgtc tcacgctata aaactggatt
     6121 tattcagccg tttcttcggc tgattcttta gccaaacgtt ctggtttaga agtaacgatg
     6181 atttttccat ctttatccag ttttgctttc aactcatgga tagaagggtg atcaagataa
     6241 aattcagcga taccatcttc gatttgttct tggatcgtgc gtcgaagagg acgagcaccc
     6301 atagatggat catagcctaa gtcaactaat ttttctttga cattcgtagg aacttcgatg
     6361 tgaagttgct gtgctgccag catttggttg acatcgtcta gcattagtgt gacgattgtc
     6421 atcaagtttt cttttgataa tgcactgaat tcgatgatcc cgtcaaaacg gttcagaaat
     6481 tctggtgtaa agtagttgtt taattggttc aatacagaac gagtaacacc ttcgcgtgcc
     6541 gcaccaaaac caacgtttgc ttctacttta ccagttccgg cattacttgt cataatgatg
     6601 atcgtatctt taaagctgac cgttcttcct tgtgcatctg tcaatcgacc atcatctaag
     6661 atttgcaaga acatatgcaa aacatctgga tgagcttttt ctacttcatc taacaagaca
     6721 agactgtaag gatttctccg tactttttct gttaattgac cagcttcttc gtatccgaca
     6781 taacctggag gagaaccaat caatttagag acactatgtt tttccatata ttcagacata
     6841 tcgaaacgaa tcattgaatc ttgtgaaccg aatagttcgt aggcaagttg tttcgcaagc
     6901 tctgttttac ctacaccagt tggtccaaca aataagaagg agccgattgg acgattctgt
     6961 ttgtttaatc caacacggtt tcggcgaatc gctttcgcta ctcgatctac agcgttatct
     7021 tgtccgatga catgagcttt caagtcatct gctaagtttt tcagttgtgt ctgttctttt
     7081 tcttttaatt cacccactgg aatacctgtc cgttgttcaa cgattttttc catatctttt
     7141 tcggtaatca caggtgtttc ctcttcagtg agctgacgtt ctttcatttt ttgaagttta
     7201 ttgatctgat cgcgataata agctgctttt tcgaaatctt cttctcgaga agctaaaact
     7261 ttttgttctt ctgcttcttg cagttttttc tcgattgttt taggatcaac gatttggatc
     7321 gtcaagtttt tcttagaacc tgtttcatct agcaaatcga tggctttatc cggcaagaag
     7381 cgatcttgga cgtaacgatt ggataaagtt gcggctgctt cgatggcctc atcagtatat
     7441 tttacatgat gatagtcttc ataacgtttt tgcaaacctt tcaaaatact gatcgtttct
     7501 tcgacagttg gttcatctac ccgtacaggt tgcatacgac gttctaaagc agcgtctttt
     7561 tcgatgatgc ggtactcatt taaggtagta gcaccaacca tttgcaattc tcctcgagct
     7621 aaagctggtt ttaaaatatt accagcatcc atatttccat caccagcaga accagcaccg
     7681 acgatttcgt gaacttcatc tatgaatagg atcacatttt ctgcttgacg gatctcttca
     7741 atcaattttt gcatacgttc ttcaaattgt cctcgtatac cagtaccttg gactaaagat
     7801 acgacatcta aacgaatgac ttctttgtcc attaattttt gcggtacatc accatctacg
     7861 attttttgtg ctaaaccttc aacaactgct gttttaccaa cacctggttc tccaatcaag
     7921 acaggattgt tcttggttcg gcgatttaaa atttcaatga ctcgtttgat ttcgtcatcg
     7981 cgtccgatca caggatcgat atcaccttgt cgtgcttgtt ctgtgatatt gattccgtat
     8041 tctcccaata aaccttctgt attgccttga ggtggctgac caccattgaa gttgttgttt
     8101 ccgccaaatt gtgttggtgg tgtttgctca taatttcctt gctgttgcat ttggcgagac
     8161 atggaacgga aaagatcgtc caaactgcca aatccaaatg gatcattttg ggccatatta
     8221 gtcagacctc cattttgttg atttttgatt ttttgataac agctctgaca gtaatcgagt
     8281 tgtgtccgct gaccattgac agtggcatac aagtgaatcg ttgcctcatt cttgccgcag
     8341 ttttgacaaa gcatgaatca ttcacgtcct ttcacaaaaa tgggtacatc catattttac
     8401 atctcgtttt tcatgaggta aaagaataac ccctagtttt ttaagagatt tatgaggtag
     8461 aaaaggtgaa atctctattg accattcctg acctttgact cttattatac taaaaataaa
     8521 aagggaagca agtaattcgt ttcatatgaa aacaaagagg gtcaaaggaa agaatgataa
     8581 acgtcaggag aagagaaagc gacaagaaaa aggaaataaa cacgtttgtt ataaaatgtg
     8641 aagaaaagga tgaaaaagaa tccggaataa tttgaatact tttatctgaa attgaatgaa
     8701 aactgttgta tctaacgaaa aaaaatgtta atctttacag atgaaagggt ggcatttgtt
     8761 aggattctct gaattactaa aaaagaaaac cctcacataa aaacaacact cacttaaagg
     8821 agaccgatta atatggaaaa gaaagaattt cacgtagtag cagaaactgg gatccacgct
     8881 cgtccagcta cactattagt acaaacagca agcaaattta actctgatgt aaacttagag
     8941 tataaaggta aatcagtaaa cttgaaatca atcatgggcg ttatgtcttt aggtgtaggt
     9001 caaggttctg atgttactat tactgcagaa ggtgctgacg aagctgatgc aatggcagca
     9061 atcgttgaaa caatgaagaa agaaggctta tctgaataat ggttgaaatg ctaaaaggga
     9121 ttgccgctag tgacggtgta gccgttgcaa aagcttacct gctagttcaa ccggatttaa
     9181 cattcagtaa ggcaacagta gaagatactg cggctgaaga agctcgtttg gatgctgcat
     9241 tagctaaatc tactgaagaa ttgcagcaaa ttcgtgaaaa agcagcgcaa agcttaggtg
     9301 aagcagaagc gcaagtattt gatgcccatt tgatggttct ttcagatcca gaaatggtag
     9361 gtcaaatcaa gcaaaatatt aaagacaaca gcgtaaatgc tgaatcagcg ctaaaagaag
     9421 taacggatat gtatatcggt atgttcgaag caatggaaga taatgcttat atgcaagagc
     9481 gtgctgcaga tattcgtgac gttgctaaac gtattttagc gcacttatta ggcgttactt
     9541 taccaaatcc atcaatgatc aacgaagaag tggtcgttgt tgcacatgac ttgactccaa
     9601 gtgatacagc acaattagat cgcaattttg taaaagcgtt tgtaacagac attggtggac
     9661 gtacttcaca ttctgctatc atggcacgtt ctttagaaat tccagcaatc gttgggacaa
     9721 aagaaattac tgctaaagtc aaagaaggcg atattttggc tgtcaacggg atcgaaggcg
     9781 atgtaatcat cgatcctaca gatgaacaaa aagctgaatt tgaaaaagca ggtgcagatt
     9841 acgcagcaca aaaagcagaa tgggaaaaat taaaaaatgc agaaactgtc acagctgacg
     9901 gcaagcattt tgaattagct gcaaacatcg gaacaccaaa agatctagtt ggtgtccata
     9961 ataatggtgg agaagcagtt ggtctgtacc gtactgaatt cctgtatatg gattctccag
    10021 acttcccaac agaagatgac caatatgaag cttacaaagc tgtattagaa ggtatggaag
    10081 gaaaacctgt agttgttcgt acaatggaca tcggtggaga taaagaacta ccatatctgc
    10141 aattacctca tgaaatgaat ccattcttag gatatcgtgc attgcgtatc agtttatctg
    10201 aacaaggcga cgaaatgttc cgtacacaaa tgcgtgcatt gcttcgtgca tctgttcatg
    10261 gtaatttacg tatcatgttc cctatggtag ctacactcaa agaattccgc gcagcaaaag
    10321 cgatctttga agaagaaaaa caaaaattga taagtgaagg caaggaagta tctgatacga
    10381 tccaagtcgg catcatgatc gagattccag cagcagcagt cttagcagac aagtttgcaa
    10441 aagaagtaga tttcttctct gtaggtacga acgatttgat tcaatacaca atggcagcag
    10501 accgtatgaa cgaacgcgtt tcttacttgt accaaccata taacccatca attttacgtt
    10561 tgatcaaaaa tgtaattgat gcagcacacg ctgaaggaaa atgggcaggt atgtgtggag
    10621 aaatggctgg agatcaaaca gctgttccat tgctagtagg tatgggtcta gatgagttct
    10681 caatgagtgc gacatctatt cttaaaacgc gtagtttaat gaaacgcttg gacacagcga
    10741 aaatggctga acttgctgat cgtgcattga aagaatgcga tatgatggaa gaagtcgttg
    10801 aacttgttca tgaatatgta aaataaacaa cgtatcaaaa gctggtgtgg cttgtaaaag
    10861 agctacgcca gtttttttgt tcttttttaa gaaaacgttt ttgtcagtaa gaatagacaa
    10921 aaat
//
LOCUS       DARBET010000082        10566 bp    DNA     linear   BCT 07-NOV-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4027
            SAMEA114569841-rid20112183.denovo.084, whole genome shotgun
            sequence.
ACCESSION   DARBET010000082 DARBET010000000
VERSION     DARBET010000082.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA114569841
            Sequence Read Archive: ERR12203666
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 10566)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10566)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001972618.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001972618.1
            Genome Coverage       :: 132x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/07/2023 13:18:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,041
            CDSs (total)                      :: 2,975
            Genes (coding)                    :: 2,908
            CDSs (with protein)               :: 2,908
            Genes (RNA)                       :: 66
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 67
            CDSs (without protein)            :: 67
            Pseudo Genes (ambiguous residues) :: 0 of 67
            Pseudo Genes (frameshifted)       :: 26 of 67
            Pseudo Genes (incomplete)         :: 28 of 67
            Pseudo Genes (internal stop)      :: 20 of 67
            Pseudo Genes (multiple problems)  :: 6 of 67
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10566
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA114569841-rid20112183.denovo.084"
                     /isolate="VRE4027"
                     /db_xref="taxon:1352"
                     /country="Denmark"
     gene            complement(<1..562)
                     /locus_tag="SCJ86_002374"
     CDS             complement(<1..562)
                     /locus_tag="SCJ86_002374"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336903.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEG1169894.1"
                     /translation="MNDEYKNDEDKMLFEEIENRCRLNFELRGKMSLIQQKKYLANKS
                     EFTLGHVEKLISDWISSRSEFTKIKQPIKFDMKKLLLNKSEIGNRDQYIRAKGQEIID
                     SLGEMRSYNYLYVTHRADGMVITVGKSSSNDIFLDGDLFYQLNINHLSGTENIILRTE
                     YGNEIFAKYDEILKNYLDWAWIIPVES"
     gene            complement(662..1171)
                     /locus_tag="SCJ86_002375"
     CDS             complement(662..1171)
                     /locus_tag="SCJ86_002375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343721.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1456 family protein"
                     /protein_id="HEG1169895.1"
                     /translation="MNNNDRLLRLRYAIDLKDTDLIKAFELGGVPLTKEEAQAVLTKV
                     QDKNKDNAENNVYEKTINNQVFDAFLNGLILLARGPQKDKPIVDTPQKSKEIKYINNV
                     LLKKLKIALSMTTDDILDVFAEAEIYPSKGEIGAFLRKEGQRNFKPCGDKYMRNFLKG
                     LGIYNRRKV"
     gene            complement(1372..2241)
                     /locus_tag="SCJ86_002376"
     CDS             complement(1372..2241)
                     /locus_tag="SCJ86_002376"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312639.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family protein"
                     /protein_id="HEG1169896.1"
                     /translation="MYGSIEAGGTKFVCAIGNDDLKVLERVSFPTTTPNETMSLVIDF
                     FNHYKEQLESIGVGSFGPIDIHRESKTYGHITSTPKTAWKNFDFVGTLNKHFEIPIAW
                     TTDVNAACYGEYVSGHGKGLSSVVYYTVGTGIGGGAIQDGIFVEGFSHPEMGHTLVKR
                     HSGDTFSGTCPFHHDCLEGLASGPAIEMRTGTKGQDLSIEDPFWEIEADYIAQCACNT
                     TLMLSPDIIIFGGGVMQQEHLKKKVQRRFLELINGYVDTPNIEEYIVTPKLADNAGTI
                     GCLTLAKDVRINS"
     gene            complement(2261..3382)
                     /locus_tag="SCJ86_002377"
     CDS             complement(2261..3382)
                     /locus_tag="SCJ86_002377"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317128.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2961 domain-containing protein"
                     /protein_id="HEG1169897.1"
                     /translation="MNCLNNIWLHKGEESRTISPENKNGEKGKAAMTASHLGIGRKGS
                     PSVPINAGETITLAEMEGPGILRHFWMTVTDKTIAGSFVLRDLILRIYWDDESTPSVE
                     SPLGDFFCNGFGTRCEVNSLPIVVNPTSGMNCYFEMPFRKKAKITLTNEHPEFIKHIF
                     YTFNYALTEVPDDAMYFHAKWNREPITTKGKDYTLIDGIKGTGYYVGTYFALCALERY
                     WWGEGEFKFYIDGDEDFPTITSTGAEDYFGGAWAFHKKNFGERAYAQNFNTLFMGYPF
                     QSKRDHTRDYFSAGKPNPNPVHGFGDDALPMHGLYRWHLPDPISFKENLRVTFQTLGN
                     DDIKLYERQDDISSVAYWYQKEPHEFFEKLLDREERIPR"
     gene            complement(3384..3779)
                     /locus_tag="SCJ86_002378"
     CDS             complement(3384..3779)
                     /locus_tag="SCJ86_002378"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312636.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS fructose transporter subunit IIA"
                     /protein_id="HEG1169898.1"
                     /translation="MRRLIIASHSILAEGFKETLEFLTGMHNQISSLCAYVEEIDDID
                     EQIKELVDTTDEIIVLTDMAGGSVNQKFFTYISDRVHVIAGMNLPLAMSIALQLGNDN
                     LDIGAIIAEAQQQIIYMNNQKSEERIEDE"
     gene            complement(3798..4604)
                     /locus_tag="SCJ86_002379"
     CDS             complement(3798..4604)
                     /locus_tag="SCJ86_002379"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350294.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS system mannose/fructose/sorbose family
                     transporter subunit IID"
                     /protein_id="HEG1169899.1"
                     /translation="MNKMSEKYQVTKKDLRKANYRWLMSVCTFNYQTQQGASVTYALS
                     PILRKLYKDDDEYIEALNNHFQYYNTQPWLAAIILGACVAMEEKQGLEAKDAINDFKV
                     GTMGPIAGIGDSLLMTMIPTIMGSIAAYMALENNPVGIFLWFILIMVIFFFRMRGFEF
                     GYKQGMKIVTEYGEKINYLTEAASVLGLTVVGSLIASVISVSTPLKFTFGEVSMKIQP
                     MLDKILPTLIPVIITAAAYYLLSKKKISMTILILLVIVFAMVMAAFGILA"
     gene            complement(4597..5385)
                     /locus_tag="SCJ86_002380"
     CDS             complement(4597..5385)
                     /locus_tag="SCJ86_002380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HEG1169900.1"
                     /translation="MHFTIFQIIMLTILAFIKHVDYYGIPMIFVNYAVFWGLFTGILM
                     GDIKTGLIVGGTVQLMSLGVAGFGGSSVPDYGTCAIIATAFGSQLGVEAGLAIGLPVG
                     MLGVQLDVIVKILNGFVVRKSQEYANKREFKKMNSVLWLGPIFFGLSAAIPVFIAVTL
                     GQSAVNYVLDVLPNWFMDGLTLAGKMLPAVGIAMLLRFMPTGRYISYLLVGFFLAAFL
                     KVPIIGAAIIGFALAFNVYHRAEKDAQITASAGASDVGFIGEDE"
     gene            complement(5399..5875)
                     /locus_tag="SCJ86_002381"
     CDS             complement(5399..5875)
                     /locus_tag="SCJ86_002381"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312631.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HEG1169901.1"
                     /translation="MSIVMSRVDNRLLHGIIVTQWAPMSKANRVMVIDDSVANNDIQK
                     SSMRLARPAGMAVSIITEETAIANFKKGKYDQEKVFVIVKEPETILHLVDNGIRIDRL
                     IVGGTVVKDNALQLSSRAYASDENISTYKKLLDSGVEIVTQFVPADKAQEIQSKLG"
     gene            complement(5947..8604)
                     /locus_tag="SCJ86_002382"
     CDS             complement(5947..8604)
                     /locus_tag="SCJ86_002382"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323457.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma 54-interacting transcriptional regulator"
                     /protein_id="HEG1169902.1"
                     /translation="MEELKERIVNYVIENSEIKNKWGNNTAQKISEKLNVSRNIVSQY
                     LNEFVKDGEFGKINTRPVIFFSLPEKVPNQQIFSSSEEAEKWYDSSFRVGIERVIGSQ
                     GSLRNVIAQIKAAIYYPPNGLPILLHGETGTGKSYLANYVYKDLLANNYIEEKASFIS
                     VNCSEYANNPELFLSNFFGHVKGAYTGAEDGRKGLAELANNGVLFLDEIHALSNECQE
                     KLFQFMDTRKFHRMGDNENWYTSSCWIIFATSEPPEDKLLSTLLRRIPVKLTLPTLDS
                     RSLYERKQLIKIFFKSEEERLAKKILISQQLLDFLLNYRFSENVGNLKNIIQLTCANA
                     FYFLDDLEELYIQMYHLPQWVNFNGMKVMKRQNELKYIQMDDLEKVDEVFNINELIEY
                     LYQTWKKDTDKQQLFEKYDRYFRQKNVDIQNNSALTYPTYQEIRFRQYVKEIERDFQI
                     EFDYYYLQITSIFLNSWIYQIQLTSDDKLYSFINDINSWCSREVQLVNRFIQIGDIYL
                     TKIQKNVLVILFKLMCSKEFQQKRLALILAHGDLTATCIASTANRIVERYIYDAIDMP
                     LMTSSSEIADKINRYLQECTGYEEIILLVDMGSLEKIYLNLELDAQKKFGLLNNVSTR
                     MAIDVANQILLDLPLKQILEQVKENQNVDYIYESSNIKKDMIVCSCASGLGTSFKLKK
                     ILEDSFPRDTDLVIETCDYYSLVNENYLSELRKENNILFIVGTLNPHLEDVEFFSIED
                     MILGMNISKFSNQLNRHFNDNELKVLNNNILKNFSLTNLMEQLTILNPTKLLERVSDA
                     IYILQNDLGISFDNNTCFGLYVHISCLIERLVKQNTLEDEIYFNETSEEFQKFQTHFK
                     QSFSVVEHYYSVDIPIHEVKYVYDYVKRA"
     gene            complement(8623..9027)
                     /locus_tag="SCJ86_002383"
     CDS             complement(8623..9027)
                     /locus_tag="SCJ86_002383"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349740.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I restriction-modification system subunit M
                     N-terminal domain-containing protein"
                     /protein_id="HEG1169903.1"
                     /translation="MGAELNQKLFSAADNLRSKMDASEYKNYLLGLIFYKYLSDRLLE
                     QVVLLADESLEEYDTVSKQTMLYRELLSDEESKEDLIATIVDILGYAISPEYLFNVLA
                     DQAKQATFQLNDLNKAFLASHIYGKILSAKFE"
     gene            complement(9259..9516)
                     /locus_tag="SCJ86_002384"
     CDS             complement(9259..9516)
                     /locus_tag="SCJ86_002384"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTP pyrophosphokinase"
                     /protein_id="HEG1169904.1"
                     /translation="MEDSPELFPMLKDRLNELTEKRRQLQIRENEILGIFQQQGEPIQ
                     VKDVQQILTSLDCFLIQSEKKQVKALYHTFIEKITFDPNQK"
     gene            complement(9641..9973)
                     /locus_tag="SCJ86_002385"
     CDS             complement(9641..9973)
                     /locus_tag="SCJ86_002385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324517.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEG1169905.1"
                     /translation="MPLDLSTGRKIRFSEIQYEGIGFTLIDLKNSTFEIKEGLDLKNL
                     LCADDKIKKIQLYKVPILTPTVLTMLEMENNINYIDSGSDLLIRWQVITNFLMELKKY
                     SNTQTKFS"
BASE COUNT         3291 a         2141 c         1472 g         3662 t
ORIGIN      
        1 ctgactcaac cggaattatc caagcccagt ctaaataatt ttttaaaatc tcatcatatt
       61 ttgcaaatat ttcattgcca tattccgtcc ttaaaattat attttcagtg ccagacaagt
      121 gatttatatt gagttgataa aaaagatctc catctaaaaa aatatcatta gaagaagatt
      181 tccccacagt aatgaccatt ccatcagctc tatgtgttac atacaaataa ttgtatgacc
      241 tcatttcacc taatgaatca attatttctt gccctttagc tctaatatat tgatctctat
      301 tacctatctc actcttattt aacaaaagtt ttttcatgtc aaatttaatc ggctgtttta
      361 tttttgtaaa ttcgcttcta ctactaatcc aatccgagat taatttttca acatgtccca
      421 gagtaaactc tgatttattc gctaaatact ttttttgttg tattaaagac atctttcccc
      481 gaagctcaaa attgagccta catcgattct ctatctcttc aaaaagcatc ttatcttcat
      541 cattcttata ttcatcattc ataaaaccaa tccagctcct cgattaagat aatattttaa
      601 tctcatacaa aaactagctc tcatcaaaac aaccattgtt ttgatttctc atgaaatttc
      661 tttatacttt tctccgatta taaattccta accccttgag aaaattacgc atatatttat
      721 ctccgcaagg cttaaaattt cgttggcctt cttttctcaa aaatgccccg atttcgcctt
      781 ttgatggata gatttctgcc tcagcaaaaa cgtctaaaat atcatcagtg gtcatagaca
      841 aggcgatttt taattttttt agcaacacat tgttgatata tttgatttcc ttgctttttt
      901 gcggcgtgtc tactatcggc ttatcttttt gcggaccacg cgccaagaga atcaggccat
      961 ttaggaatgc gtcaaaaact tggttgttga tggttttttc atacacatta ttttctgcat
     1021 tatctttgtt cttatcctgt actttggtca gtaccgcctg agcttcttct ttagtcagtg
     1081 gtacaccgcc tagctcaaaa gctttaatca aatccgtatc tttcaaatca atcgcataac
     1141 gcaatcgcaa taacctatca ttgttattca tttgttcctc cgaagtattt agtctttact
     1201 ttcttacgta ctttttgtct tgctaagatt tttaggatca atgataaaaa ccgcatcact
     1261 tatgataagg cgctgggtat catgagtgaa tgatattgct gaatacaaat agtccgacca
     1321 cattaagttg atcggacttc ataagcattt tattgactaa tacttttttg cttaagaatt
     1381 gattctaaca tcttttgcta aagttaagca tcctatagtt cctgcattat ctgctaactt
     1441 aggtgtgaca atgtattctt cgatatttgg cgtatcgaca tagccgttaa ttagttctaa
     1501 aaaacgtctt tgtactttct ttttcagatg ttcttgttgc atgacaccac caccaaaaat
     1561 tataatatct ggtgaaagca tcagggtcgt attacaggca cattgtgcaa tataatccgc
     1621 ctcgatttcc cagaacggat cttctataga aagatcttgt cccttggttc ccgtacgcat
     1681 ctcgatagcg ggtccagatg ccaacccttc gagacaatca tgatgaaaag gacaagtacc
     1741 agagaaagta tctcctgaat gtctcttaac taacgtatgc cccatttctg gatggctaaa
     1801 cccttcaaca aaaatcccat cttgtatagc tcctccacca attccagttc caacagtgta
     1861 ataaacaaca ctggataaac cttttccatg acctgaaaca tactccccgt aacaagcagc
     1921 attcacatcg gttgtccagg caattggaat ttcgaaatgc ttattcaacg ttccaacaaa
     1981 atcaaaattt ttccaggcag tttttggagt agaggtgata tgtccatagg tctttgattc
     2041 acgatggata tcaatcggtc caaatgaccc tactccaatg ctttctaatt gttctttata
     2101 gtgattaaag aaatcaatta ccaaactcat tgtttcattc ggtgtagttg taggaaaact
     2161 cactctttct aaaaccttta aatcatcatt gccaatggca caaacaaatt tagtcccacc
     2221 tgcttctatt gagccataca tttcttactc caccgtcctt ttatctagga attcgttctt
     2281 ccctgtctaa tagtttttcg aaaaactcat gtggttcctt ttggtaccaa taggcaacag
     2341 aagaaatatc gtcttgacgc tcataaagct tgatgtcatc atttcctagg gtttggaaag
     2401 taacacgtaa attttctttg aaactaatgg ggtcgggtaa atgccagcgg tacaacccat
     2461 gcatcggtaa agcatcatcg ccaaaaccat gaactggatt tggattaggt tttccagcac
     2521 taaaataatc ccgggtgtga tctcttttag attgaaaagg atagcccata aatagcgtat
     2581 taaaattttg agcatatgcc ctttctccaa agttcttttt atggaaagcc caagcgccgc
     2641 caaaataatc ttctgctcct gtagatgtta ttgtagggaa atcttcatct ccatctatat
     2701 aaaacttaaa ttctccttcc ccccaccagt atctttccaa tgcgcacaaa gcaaagtagg
     2761 ttcctacata atacccagtg cctttgattc catcaataag ggtgtaatct tttccttttg
     2821 tcgtgatggg ttcacggttc catttagcat gaaaatacat cgcatcatcc ggtacttcag
     2881 tcaatgcata attaaatgta taaaaaatgt gcttaataaa ctcaggatgt tcattcgtta
     2941 aagtaatctt agcttttttt cgaaatggca tctcaaaata gcaattcatc cctgatgttg
     3001 gattcactac gattggtaag gaattcactt cgcatcttgt cccaaaccca ttacaaaaaa
     3061 aatctcctaa tggactttct acagaaggag tggattcatc atcccaataa atacgcaaaa
     3121 ttaaatcacg taagacgaaa ctcccagcga ttgttttgtc tgtcactgtc atccaaaaat
     3181 gacgtaaaat tccagggcct tccatttctg ctaaagtaat tgtctctcca gcatttatcg
     3241 gaacagaagg tgaccctttt ctcccaattc ctagatgact cgctgtcatt gccgctttcc
     3301 ctttttcacc atttttattt tctggtgaaa tagttctact ttcctctccc ttgtggagcc
     3361 atatattatt taaacaattc attttattcg tcctctatcc tttcttcact tttttgatta
     3421 ttcatataga tgatttgttg ttgtgcctcc gcaataattg ctccaatatc taaattatca
     3481 tttcctaact gcaaagcgat tgacattgcc aagggaagat tcattcctgc aattacgtgc
     3541 actctgtcag aaatataggt aaaaaacttt tgatttacac ttcctccagc catgtcagtt
     3601 aaaacaatta tttcgtctgt agtatccact aactctttaa tttgttcatc tatatcatca
     3661 atttcttcga catatgcaca taagctacta atttgattat gcatacctgt taaaaactct
     3721 agtgtctctt taaaaccctc agctagtatt gaatggctag caatgatcaa tcttctcata
     3781 attcaaatcc ttttccttta cgctaatatt ccaaatgcag ccataaccat cgcaaaaaca
     3841 ataacgagta gaatcaaaat ggtcatactg atttttttct tactgagtag ataatatgct
     3901 gcagcagtaa taattactgg aattaatgtt ggtaaaattt tgtccaacat tggttggatt
     3961 ttcatactaa cttcaccaaa agtgaattta agtggcgttg aaacactgat aactgaagca
     4021 attaatgatc caactactgt tagccccaat actgaagcag cttcagttaa ataattaatc
     4081 ttttcaccgt attcagtaac aatcttcatt ccctgcttat aaccaaattc aaagcctctc
     4141 attcggaaaa agaagattac catgattaaa atgaaccata aaaagattcc tacaggatta
     4201 ttttctaaag ccatataagc cgcaattgat cccatgatgg ttgggatcat cgtcatcaaa
     4261 agagagtccc caataccagc aattggaccc atcgtaccaa ctttaaagtc attaatcgca
     4321 tcttttgctt ctaatccttg cttttcctcc attgcaacac aagcacctaa aataatggct
     4381 gccagccaag gctgtgtatt gtaatactga aagtgattat ttaaagcttc gatgtattca
     4441 tcatcatctt tgtataattt tcttaaaata ggtgacaatg cgtaagtcac agatgcacct
     4501 tgctgtgttt gataattaaa ggtacaaact gacatcaacc aacgatagtt cgctttccta
     4561 agatcctttt tcgttacttg atatttttcg ctcattttat tcatcctccc caataaaccc
     4621 aacatcagaa gctcccgcag aagcagtgat ttgtgcatct ttttcagctc tatgatacac
     4681 attaaatgct aaagcgaatc ctatgatcgc cgctccaata ataggtacct tcaaaaaagc
     4741 ggctaagaaa aatccaacaa gtaaataact aatgtatcga cctgtaggca taaagcgtaa
     4801 caacatcgca ataccaacag caggcaacat ttttccggct aatgttagcc catccataaa
     4861 ccaattaggt aaaacatcca aaacgtaatt aacagcagac tgtcctaaag taacggcaat
     4921 aaatactgga attgctgcag ataaaccaaa gaaaattggc cccaaccaca aaacactatt
     4981 cattttctta aattctcgtt tattcgcata ttcctgtgat ttgcgaacaa caaaaccatt
     5041 taagattttg acaattacat ctagttgcac ccctaacata cctacaggta gtcctattgc
     5101 taatccggct tctactccaa gctggctacc aaatgccgtt gcaataattg cgcacgttcc
     5161 atagtccggt acagatgagc caccaaaacc ggcaactcct aatgacatca attgaactgt
     5221 accgccaact atcaaacctg tttttatatc acccatcaaa attcctgtga ataatcccca
     5281 aaaaactgcg taatttacaa agatcatcgg aatcccataa taatcaacat gtttaataaa
     5341 tgctaatata gtcagcataa taatttgaaa aatagtaaaa tgcattatcc ttactccttt
     5401 atcctaattt tgattgaatt tcctgtgcct tatccgccgg aacaaattgt gtaacaattt
     5461 ctacaccaga atccaacaat tttttatacg tgctgatatt ttcatctgat gcataggctc
     5521 tagaagatag ctgtaatgca ttatctttaa caacagtacc gccaacaatt agtcggtcaa
     5581 ttctaatacc gttatctact aaatgaagga ttgtttccgg ctctttcacg atgacaaata
     5641 ctttttcctg atcgtatttc ccctttttaa aattcgcaat cgctgtttct tcagtgataa
     5701 ttgatacagc cattccagca ggtctagcta aacgcatact tgatttttgg atgtcgttat
     5761 ttgcgacaga atcatcgatc accatcactc tgtttgcttt agacattggt gcccattgcg
     5821 tgacaataat cccatgcaat aaacggttat caactctcga cataacaata ctcataaata
     5881 cttcctcctt tatctttcat tttcttacaa gcaattacca tgccaaaaca aaaagaactt
     5941 gataaatcaa gctcttttta cataatcata aacgtattta acctcatgaa tcgggatgtc
     6001 gacactataa taatgttcaa cgacactgaa agattgctta aaatgtgtct gaaatttttg
     6061 aaattcctca ctcgtttcat tgaaataaat ttcatcttcc aaagtatttt gttttactaa
     6121 tcgttcaatt aagcaactaa tgtgaacata taatccgaaa cacgtattat tatcaaatga
     6181 aattcctaga tcattttgta agatgtatat agcatctgaa acccgttcta ataatttagt
     6241 cggatttaat atggtcaatt gctccattaa attcgttaga ctaaaattct tcagaatatt
     6301 attgttcaaa actttcagtt cattatcatt aaagtgtcta ttcaattgat tggagaattt
     6361 acttatattc attcctaaaa tcatatcttc aattgaaaag aattctacat cttctagatg
     6421 tggatttagt gttccaacaa taaaaagaat attgttctct ttacgaagct ccgataaata
     6481 gttttcattc actaaagagt aatagtcaca cgtttctatg acaagatcag tatcccttgg
     6541 aaagctatct tcaagtatct tttttaattt aaacgaagtt cctaatccgc ttgcacaaga
     6601 acagacaatc atatccttct taatatttga tgattcataa atataatcca cattttgatt
     6661 ttcttttact tgttcaagaa tctgtttaag cggtaaatct aataatatct gatttgcaac
     6721 atcaatagcc attcttgttg aaacattgtt tagtagtccg aatttttttt gagcgtctag
     6781 ttccaaattt agataaattt tctccagtga acccatatca acaagtaaaa taatttcttc
     6841 atatccggta cactcttgta agtatctatt aattttgtct gcaatctcac tacttgaagt
     6901 cattagtggc atatcaatgg cgtcgtatat atatctctca acgatcctat tagcagtact
     6961 tgctatacag gttgccgtta gatctccatg agccaaaatc agtgctaaac gtttttgctg
     7021 aaattcttta ctacacatta acttaaataa aataaccaaa acattttttt gaatttttgt
     7081 taaataaata tcccctattt gaataaaacg attgactaat tgaacttctc gactacacca
     7141 actattaata tcattgatga aggaatataa tttatcgtcc gatgttaatt ggatttgata
     7201 aatccaagaa ttcaaaaaga tagacgttat ttgaagataa taatagtcaa attctatttg
     7261 aaaatctctc tctatttcct taacatactg tctaaatcga atctcttgat aggtgggata
     7321 tgttaaagct gaattatttt ggatatcaac attcttttgt ctaaaatatc tatcatattt
     7381 ctcaaaaagc tgctgcttat cagtgtcttt cttccacgtt tgataaagat actctataag
     7441 ttcgttgata ttaaaaactt cgtctacttt ttccaaatca tccatttgaa tatattttaa
     7501 ttcattctgt cttttcataa ccttcatgcc gttgaaattc acccattgtg gtaaatgata
     7561 catctgaata taaagttctt ccaaatcatc taagaaataa aaggcattgg cacaggtcaa
     7621 ttgaatgata tttttcaaat tgccaacatt ttcactaaag cgatagttta ataaaaaatc
     7681 taataattgt tgagatatca gtattttctt tgctagtctt tcttcttctg atttaaaaaa
     7741 tatcttgatt aactgttttc tttcataaag agaacgactg tctaatgttg gaagtgttag
     7801 ctttacaggt attcttctca acaaagttga taatagttta tcctcaggtg gttctgatgt
     7861 tgcaaatata atccagcaac ttgatgtgta ccagttttca ttatcaccca tccgatgaaa
     7921 tttcctcgta tccataaact gaaaaagttt ctcctgacat tcattgctca atgcatgaat
     7981 ttcatctaaa aataagactc cattattagc taattctgct aaaccctttc ttccatcttc
     8041 ggctcctgta tatgcacctt ttacatgccc aaaaaaattg gacaagaaaa gttctggatt
     8101 attggcatat tcactacaat taactgaaat gaaagatgcc ttctcttcta tataattatt
     8161 ggccaacaaa tccttataca cataatttgc taaatagctt tttccagttc cagtctcgcc
     8221 atgaagtaaa atgggtaagc catttggcgg atagtagatt gctgctttaa tttgagcaat
     8281 tacatttcgc agacttcctt gacttccgat aaccctttct attccaaccc taaaagagct
     8341 atcataccat ttttccgctt cctctgaaga gctaaaaatt tgttgatttg gaactttctc
     8401 cggtaaagaa aagaagatta ctggacgagt attgattttt ccaaattctc catcttttac
     8461 gaattcattc aaatattgag agacaatatt acgactaaca tttaattttt cagatatttt
     8521 ctgtgctgta ttgttccccc atttattttt aatctcagag ttctctatga cataattgac
     8581 aatgcgttct tttaattctt ccatcacagc acctcctcga cactattcga attttgccga
     8641 cagtattttt ccataaatat gacttgctaa aaaggctttg tttagatcat tcaattggaa
     8701 cgttgcttgt tttgcttgat ccgctaagac gttgaaaaga tactctggcg aaatcgcata
     8761 ccccaaaata tcaacgattg ttgcaatcag atcttctttt gattcttcat ctgaaagtaa
     8821 ttcacgatac aacatcgttt gtttgcttac tgtgtcatat tcctctaagg attcatctgc
     8881 taaaagaacc acttgttcca acaaacggtc ggataaatat ttatagaaaa tcagccccaa
     8941 taaataattt ttatattctg acgcatccat tttactacgg agattatctg ccgcactaaa
     9001 taacttctga tttaattctg cacccaaaaa gatacactcc tatctcttat ttaaagttgc
     9061 tatataccct cttattttag catactcagt tttagaaaaa gcaaaaaaat aaagaaactt
     9121 attcttgata acaagctacg tgacgtatat ccctaacaca aaaatagcca tatcctttgg
     9181 tttggatacg gctatttgat attactcatt caattatcaa atcacagtag gtgtgaatct
     9241 catcgggatc ggcaatatct acttctgatt aggatcaaag gtgattttct caataaaggt
     9301 gtgatacaaa gctttaactt gcttcttctc actctggata agaaagcaat ccaaactagt
     9361 caatatctgt tggacatctt tgacttgaat cggttcccct tgttgttgaa agatccctaa
     9421 gatttcattc tctcgaattt gtaactgtct tcgtttttca gtcaattcat ttaaacgatc
     9481 ttttaacatc ggaaacaatt cgggactatc ttccaaagca gcttcctgtt tttgctctaa
     9541 tagttagatt ttgcgtacca ttttcttctt ttattttcct tcgattgctg attttaatat
     9601 agtctaaagg aaatttatat ttattgtatt ctttataact tcaagagaac ttcgtttgag
     9661 tattactgta tttttttaat tccataagaa aattagttat tacctgccac ctaatcagca
     9721 aatctgaacc tgagtcaata taattaatat tattttccat ctccaacatt gtaagtactg
     9781 ttggtgttaa tatagggact ttgtacaatt gtattttttt gattttatca tctgcacaaa
     9841 ggagattttt taaatctaat ccttctttta tttcaaaagt tgaatttttt aaatctataa
     9901 gagtaaaacc aattccttca tactgaattt ctgaaaatcg aatttttctc ccagttgata
     9961 gatcaagggg cagaaaacct tttattacat tgtgagtgtc accatcattt acaatgtcat
    10021 taggaatttt gattttagaa ttctgtggag cacctattgg aagataataa ttcattataa
    10081 ttttgtagtc ttcatgagga aataaagtac ttttaggatt gctaagatat tctcgtaccg
    10141 aatctaagaa tggcttatcc attgaatctt catccccatt taaactaaaa aacattccta
    10201 caattgcttt tgcaactcta agtgaattca ttttctttat tgaatgtgtt aaatcagact
    10261 taccattctt taaacttgta ttgttataaa ggaaattttt attgtcttct gcaaatctaa
    10321 tatattcctc aacatactca ctaccaaaaa aactattaca tttactacat aacgtattgg
    10381 aatggcaaca gtttttttga caaattttat aaggtgcaga acttctttcc gtatgctatt
    10441 ccgattgtcc ttgacaatga gcctcctcgg atgtgacgat cttcggcagg agctaacagt
    10501 taaagttaga ctgcctcgct agcgttagca catccgagct cattatcaag gattcgctgc
    10561 gccaat
//
LOCUS       DARBET010000083        10193 bp    DNA     linear   BCT 07-NOV-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4027
            SAMEA114569841-rid20112183.denovo.085, whole genome shotgun
            sequence.
ACCESSION   DARBET010000083 DARBET010000000
VERSION     DARBET010000083.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA114569841
            Sequence Read Archive: ERR12203666
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 10193)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10193)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001972618.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001972618.1
            Genome Coverage       :: 132x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/07/2023 13:18:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,041
            CDSs (total)                      :: 2,975
            Genes (coding)                    :: 2,908
            CDSs (with protein)               :: 2,908
            Genes (RNA)                       :: 66
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 67
            CDSs (without protein)            :: 67
            Pseudo Genes (ambiguous residues) :: 0 of 67
            Pseudo Genes (frameshifted)       :: 26 of 67
            Pseudo Genes (incomplete)         :: 28 of 67
            Pseudo Genes (internal stop)      :: 20 of 67
            Pseudo Genes (multiple problems)  :: 6 of 67
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10193
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA114569841-rid20112183.denovo.085"
                     /isolate="VRE4027"
                     /db_xref="taxon:1352"
                     /country="Denmark"
     gene            120..626
                     /locus_tag="SCJ86_002386"
     CDS             120..626
                     /locus_tag="SCJ86_002386"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor"
                     /protein_id="HEG1169906.1"
                     /translation="MIFTGYKKKRQIEKYRHLLNGLIETDYQKMFRITMNYVHDKEEA
                     LDVMQDSFHKALNYFEKEKEIEHFSAWFYRILIRTALDSWRRQKRTPVDLFEIQDIPQ
                     QLPLETSVAELHMILDKIDSPEKEILILYFFEGFRLKEIATILDMNENTVKTKMYRSL
                     NALRQILE"
     gene            643..1542
                     /locus_tag="SCJ86_002387"
     CDS             643..1542
                     /locus_tag="SCJ86_002387"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296447.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3298 domain-containing protein"
                     /protein_id="HEG1169907.1"
                     /translation="MYSQKENLKKLKKTYKEQAIPKGLKEEIRQRFIIEEQSFIKAKR
                     RKRRLQGFGVSLAIVMITTSSLLFNSQVRSFAEDLPILGSVIELILGERFTDRSEKID
                     IQVPKINTQNEKENKTIHGLNQKYFREGQADFEKAEKEYGNFETDHYQVLGDYQKIVD
                     DNRFLVIERQITQTAADSHVEKRYDTIDKKNSVQLSLPLLFKDDTYLSVLTKEVKRQM
                     AEQVKEDPSKYYWTEQDIQEGTIEKPTLVTPTRSFYLNKEHQLVLTFSQYEIAPGYMG
                     TPEFVIPKSVTKTILASEDYLDH"
     gene            1629..2513
                     /locus_tag="SCJ86_002388"
     CDS             1629..2513
                     /locus_tag="SCJ86_002388"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295129.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide deacetylase family protein"
                     /protein_id="HEG1169908.1"
                     /translation="MKRNYLVISIIALSVLLFFEITSVASLITEVKGQTHSSEHSSQS
                     FKQSEQSTIKSQSTETANVKEAIQESAEKVQSQSSQSTQDSTKTSRDPAVKSVAISFD
                     DGPGATTTPQLLRILKEKNVHATFFVLGENTAQHPEIVKQTAEAGHEIGNHTYDHQNL
                     ATLSAQSMTEEVTKADTEIKKVTGKTPTFVRPPYGSVTNVGATIIQRPIIEWSVDSED
                     WKTRNPDLILQKIQATVYDGAIILFHDIYPETIRAVPQVIDYLKEQGYRITTVGDLLG
                     HPTAVENYYGRNDHRPVQ"
     gene            complement(2705..2776)
                     /locus_tag="SCJ86_002389"
     tRNA            complement(2705..2776)
                     /locus_tag="SCJ86_002389"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(2741..2743),aa:Arg,seq:ccg)
     gene            2836..4170
                     /gene="rpoN"
                     /locus_tag="SCJ86_002390"
     CDS             2836..4170
                     /gene="rpoN"
                     /locus_tag="SCJ86_002390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase factor sigma-54"
                     /protein_id="HEG1169909.1"
                     /translation="MKFEQNFSQKQQQTQKLAMTQQLQQSIQVLQYNSEELLAFVENQ
                     AMENPLVEVVEPEWQPDYIKASSSSYEGEETNYLNQIPDTKGSLFDSLIEQVHLNYRD
                     TFLRKIVLYLVEYIDLNGFLTISLEEAGKEIGATPIQMLDALTLIQQLEPAGVGARSL
                     QECLMLQTERDDYAPELAYIVLEECFEELVERKWKEIAQRFSVDLHAVQQIFDYLQTL
                     SPSPGRIFDRSSELYIIPDVRVLVDDDKNVQVISNRKNQPNIRFQESYFKQMSQQADK
                     ETENYLKERKQEFEWLKKTILQRGDTILRVAQVIVSRQKAFFIDKERPIKPLTLKEVA
                     TEIDVHESTVSRAVNGKYLETDFGVFELKKFFTTRIPTNSTEQTEDLSADTAKKKLQE
                     LVDQEDKNKPLSDQKLVELLKKDEIAISRRTVAKYRDLLGIPSSSKRKRYDN"
     gene            4319..5410
                     /locus_tag="SCJ86_002391"
     CDS             4319..5410
                     /locus_tag="SCJ86_002391"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002316430.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="central glycolytic genes regulator"
                     /protein_id="HEG1169910.1"
                     /translation="MGRFKSTLDVKCPTKEIAMLDEIKMIESVAPDIIEVLQERYKIL
                     RNIYWMQPIGRRSLSESMGITERVLRTETDLLKNLNLIDTSKSGMTLTTKGEEVYQSL
                     ENFMDQLLGTHQTEQQLAEYFGNQRCIVVSGNSEEQTKVADAFGEALSEALDRLLPEG
                     ENIIAVMGGTTMAVVAEQLSNLENKKRHNLFVPARGGIGEAITVQANSVSARMAAKAN
                     GNHRALYVPEQLSLATYNSLLNEPSIQEVLNLISEANCVIHSIGRALHMAARRKMTEK
                     ELVMLKQANAVAESFGYFFNEKGEVVYKVPRIGIELQDLEKVPIIMAIAGGKSKAKAI
                     RAYMKNAPKQTWLITDEAAANEILKGVTL"
     gene            5451..6452
                     /gene="gap"
                     /locus_tag="SCJ86_002392"
     CDS             5451..6452
                     /gene="gap"
                     /locus_tag="SCJ86_002392"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010755973.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I glyceraldehyde-3-phosphate dehydrogenase"
                     /protein_id="HEG1169911.1"
                     /translation="MTVKVGINGFGRIGRLAFRRIQDVDGIEVVAINDLTDAKMLAHL
                     LKYDTTQGRFNGTVEVHEGSFNVNGKEVKVLANRNPEELPWGELGVDIVLECTGFFTS
                     KSAAEKHLTAGAKRVVISAPGGNDVPTIVYNTNHETLTGKETVISGASCTTNCLAPMA
                     KTLNDKFGVVEGLMTTIHAYTGDQMTLDGPHPKGDFRRARAAAANIVPNSTGAAKAIG
                     LVIPELNGKLDGAAQRVPVPTGSLTELVTVLEKEVTVDEINAAMKEASNESYGYNTDE
                     IVSSDIVGMTYGSLFDATQTKVMTVGDKQLVKTVAWYDNEMSYTAQLVRTLEYFANL"
     gene            6562..7752
                     /locus_tag="SCJ86_002393"
     CDS             6562..7752
                     /locus_tag="SCJ86_002393"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005228679.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglycerate kinase"
                     /protein_id="HEG1169912.1"
                     /translation="MAKKTVKDIDLKDKKVLVRVDFNVPLKDGVITDDTRIKAALPTI
                     NYVLEQGGKAILFSHLGRVKTEEDKEGKSLAPVAKRLGELLGKEVTFVPETRGEQLEE
                     AIRNMNDGDVVVFENTRFEDIDGKKESKNDTELGKYWASLGDVFVNDAFGTAHRAHAS
                     NVGIASTGIPTVAGFLMEKEIKFIGEAVEEPKRPMIAILGGAKVSDKIGVIENLIPKA
                     DKILIGGGMTYTFYEAKGIKIGNSLVEADKVELAKELIEKAGDKLVLPIDNVCAPEFS
                     NDVETQVIEGDIPDGLMALDIGPASVKLFADTLQGAKTVVWNGPMGVFEMSNFAKGTI
                     GVCEAIANLEGATTIIGGGDSAAAAEQLGFADKFTHISTGGGASLELLEGKELPGLAA
                     INDK"
     gene            7856..8611
                     /locus_tag="SCJ86_002394"
     CDS             7856..8611
                     /locus_tag="SCJ86_002394"
                     /EC_number="5.3.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005878431.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="triose-phosphate isomerase"
                     /protein_id="HEG1169913.1"
                     /translation="MRKPIIAGNWKMNKTASEAKEFAEAVKNKIPSNDKVDSVIGSPA
                     LFLQELMTSAEGTELKISAQNCYWENSGAFTGETSPAALADLGVHYVIIGHSERREYF
                     HETDEDINKKAKAIFANGMTPILCCGESLETYEAGKTAEWIEGQITAGLADLSAEQVS
                     NMVIAYEPIWAIGTGKSADAQIADEICGVVRQTVEKLYGKEVSEAVRIQYGGSVKPEN
                     IAEYMAKENVDGALVGGASLQADSFLSLLDAVK"
     gene            8727..10025
                     /gene="eno"
                     /locus_tag="SCJ86_002395"
     CDS             8727..10025
                     /gene="eno"
                     /locus_tag="SCJ86_002395"
                     /EC_number="4.2.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013773781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphopyruvate hydratase"
                     /protein_id="HEG1169914.1"
                     /translation="MSIITDVYAREILDSRGNPTIEVEVYTESGAFGRGMVPSGASTG
                     EYEAVELRDGDKARYGGKGVTKAVDNVNNIIAEAIIGYDVRDQMAIDKAMIALDGTPN
                     KGKLGANAILGVSIAVARAAADYLEVPLYHYLGGFNTKVLPTPMMNIINGGSHADNSI
                     DFQEFMIMPVGAPTFKEALRMGAEVFHALASILKARGLATSVGDEGGFAPNLGSNEEG
                     FEVIIEAIEKAGYVPGKDVVLAMDAASSEFYDKEKGVYVLADSGEGEKTTDEMIKFYE
                     ELVSKYPIISIEDGLDENDWDGFKKLTDVLGDKVQLVGDDLFVTNTQKLSEGIEKGIA
                     NSILIKVNQIGTLTETFEAIEMAKEAGYTAVVSHRSGETEDSTISDIAVATNAGQIKT
                     GSLSRTDRIAKYNQLLRIEDQLGEVAEYKGLKSFYNLKNK"
BASE COUNT         3565 a         1886 c         2112 g         2630 t
ORIGIN      
        1 ttttgtcaca ccttcttttt tgtatataaa aatcccaagt aaatacataa aaaataaaaa
       61 aattgaaact tttagcgcac cttgtacgtc atattaagtg aggcagaaag gagtggagaa
      121 tgatttttac tggatataag aaaaaacggc agatcgaaaa ataccgtcac ctgctgaatg
      181 gactcatcga aactgattat caaaaaatgt tccgtatcac gatgaattac gtacatgata
      241 aagaagaggc cttagatgtg atgcaagata gttttcataa agccctgaac tattttgaaa
      301 aagaaaaaga gatcgagcat tttagtgcat ggttttatcg gatattgatc cgcacggcat
      361 tagatagttg gcgcagacaa aaaaggacgc ctgtggactt atttgaaatt caagatatcc
      421 cccaacagtt acctttagaa acttccgttg cagagctcca tatgatttta gataaaatag
      481 acagtccgga aaaagagatt ctgatcctct acttttttga aggatttcgt ttgaaagaaa
      541 ttgcgactat cttggatatg aatgaaaaca cagttaaaac gaaaatgtac cgctcattga
      601 atgctttacg tcaaatccta gaatgaagga gagaagagtg ccatgtattc ccaaaaagaa
      661 aatttaaaaa aactaaagaa aacttataaa gaacaagcaa ttcccaaagg gcttaaagag
      721 gagatccgcc agcgttttat tatcgaagag caatcattta taaaagcaaa gcggagaaag
      781 agacgtctcc aagggttcgg agtatctttg gctatcgtaa tgattacaac aagtagcctt
      841 ctttttaata gtcaagttcg aagctttgca gaagatttac ccatcttagg atcagtcatt
      901 gaattgatct taggcgaacg ctttactgat cgatctgaaa aaatcgatat tcaggtaccg
      961 aagatcaata cacaaaatga gaaagaaaat aaaacaattc atggattaaa tcaaaaatac
     1021 tttcgcgaag gacaagcaga ttttgaaaaa gctgaaaaag aatatggaaa ttttgaaacg
     1081 gatcattacc aagttttggg agattatcag aaaatcgtag acgataaccg gtttctcgtg
     1141 attgaaaggc agatcaccca aacagcagca gatagccatg ttgaaaaaag atacgacaca
     1201 atcgacaaga aaaacagtgt ccagctctca cttccacttt tattcaaaga tgacacctat
     1261 ctatctgttt tgacaaaaga agtcaaacgg cagatggccg aacaagtcaa ggaagatcca
     1321 tctaagtact attggactga gcaagatatt caagaaggga cgatagaaaa accaacactt
     1381 gttacgccta cacgaagttt ttatttgaac aaagagcatc aattagtttt gactttttct
     1441 caatacgaaa ttgctcctgg ttatatgggt acaccagaat ttgtcattcc taagtccgtg
     1501 acaaaaacga ttttggcatc ggaagattat ttggatcatt agacgagaag tagttgcgac
     1561 tagcagtttc aattttcaag taagtacaag taaataataa gaagagaaaa agagaaagaa
     1621 gagatatagt gaaaagaaat tatttagtta tcagtattat tgctttatcc gtgcttttgt
     1681 ttttcgaaat tacttcggtg gcctccttga tcaccgaagt aaaaggacaa acacactctt
     1741 cggagcattc gtcacaatct ttcaaacaat cagaacaatc aaccattaaa agccaatcaa
     1801 ctgaaacagc taacgtcaaa gaagccattc aagaatcagc ggaaaaggta caaagtcagt
     1861 catcacaatc aacacaagac agtacaaaaa cgagccgaga tccggctgtc aaaagcgtcg
     1921 caatcagctt tgatgatgga ccaggagcga ccacaacacc gcagcttctg cgaatcttaa
     1981 aagaaaaaaa cgtgcatgcc acttttttcg tcttaggaga aaatacggcg cagcatcctg
     2041 aaatcgtgaa gcagacagct gaagcagggc acgagattgg caatcacaca tacgatcatc
     2101 agaatctggc cactctttct gcccagtcga tgacggagga agtgacgaaa gcagacactg
     2161 agatcaaaaa agtcactggc aaaaccccca cttttgttcg tccgccctat ggttctgtca
     2221 cgaatgtagg agcaacgatc atccagcgac cgatcatcga atggtctgtt gattcagaag
     2281 attggaagac aagaaatcct gatttgattt tgcagaagat ccaagcaaca gtatacgatg
     2341 gggcaattat tttattccat gatatctatc ctgaaacgat tcgtgccgtt cctcaagtga
     2401 tcgattatct gaaagagcaa gggtatcgca tcacgacagt tggggattta ttagggcatc
     2461 caacggctgt cgagaactat tatggcagaa acgatcatcg gccagttcag taagtgagac
     2521 aagtatcttt aataaataac acattttaat aagtagttga acacataact accttacttg
     2581 caaaggctta acaatatcta tacaggtaca acaaaaaact gtccaccaat tttctttggc
     2641 ggacagtttt cacttaaaaa taaagaagca actattcgta aattatgcta aaatagcagt
     2701 agaacgcgtc ctgagggaat cgaacccccg tctcaagaac cggaatctta cgtgatatcc
     2761 actacactaa ggacgcaagc actgggataa ttgtaactaa acagataaaa aattacaagg
     2821 ggattggaca aaaaaatgaa atttgagcaa aatttttcac aaaaacagca gcagacacag
     2881 aaactagcca tgacgcaaca gttgcaacag tccatccaag ttttacaata taacagtgag
     2941 gagctgttgg cattcgttga gaatcaagca atggaaaatc cattggtgga agttgttgag
     3001 ccggaatggc agccagatta cataaaagct tcttcatctt cttatgaagg agaagagaca
     3061 aattacctaa atcagattcc tgatacaaaa gggtctttat ttgattcatt gatcgaacaa
     3121 gtccatttga attaccgaga tacttttttg aggaaaatcg ttctttatct tgtagaatac
     3181 atcgatttga acggattttt gacgattagt ttagaagaag ccggaaaaga gattggagct
     3241 acgcctatcc aaatgctcga tgcgttgact ctgattcagc aattagaacc agctggagta
     3301 ggtgcacgta gtttgcaaga atgtctcatg cttcaaacag aacgagatga ttatgcgccg
     3361 gaattagctt atatcgtgtt ggaagaatgt tttgaggaac tggtggagcg aaaatggaaa
     3421 gaaatcgcac aacgtttttc tgttgatcta catgctgttc agcagatatt cgactattta
     3481 caaacgttga gtccaagccc tggccggatt ttcgaccgta gcagcgaatt gtacattatt
     3541 cctgatgtcc gggtattagt ggacgacgat aaaaatgtac aagttatctc aaaccgaaaa
     3601 aatcagccaa atatacgctt tcaagagagt tacttcaaac aaatgtcaca acaagcagat
     3661 aaagaaactg aaaattattt aaaagaacgg aaacaggaat tcgaatggtt gaagaaaacg
     3721 atcctacaac gtggggatac aattttgcgg gttgctcaag tcattgtttc ccggcaaaaa
     3781 gctttcttta tcgacaagga acgtccaatc aagcctctta cgttaaagga agttgcaact
     3841 gagatagatg tgcatgaatc cacggtcagt cgtgcagtca acgggaaata tttggaaaca
     3901 gacttcggtg tatttgaatt aaaaaaattt ttcactacac gaattcctac gaatagtaca
     3961 gaacagacag aagacctttc agcagataca gcgaagaaaa agctgcagga attagtcgat
     4021 caagaagaca aaaacaagcc gttgtctgac cagaaattgg ttgaattgct gaaaaaagat
     4081 gagatagcga tctcaagacg aacagtagca aaatatcgtg atttattagg aatcccgagt
     4141 tcatcaaaaa gaaaacgtta tgataactaa tcagtcaaaa gactagaaaa agtatttcac
     4201 tttttctagt ctttttagtt gaaaaacgag agcaaaactg ttagacttac agtcgtgagg
     4261 ttgagatttc cctttattga tgtgaaattc gaatagttaa tttctttttt tatcgtatat
     4321 gggtcgtttt aagtctacat tggacgtaaa gtgtccaact aaggagatcg ctatgctgga
     4381 tgaaataaaa atgattgaat ctgttgcacc agatattatc gaagtgttgc aagaacgcta
     4441 taaaatttta cgaaatatct attggatgca gccgattgga cgtcgaagtt tatcggaaag
     4501 tatggggatc acagaacgag tattgcgaac agaaacagat cttttgaaaa atctgaattt
     4561 gatcgacacc tcaaaaagcg gtatgacgct gacaactaag ggcgaagaag tttatcaaag
     4621 ccttgaaaac ttcatggatc agcttttagg tacgcatcaa accgaacaac agttggcaga
     4681 atattttggt aaccaacgct gtatcgttgt ttcaggaaac agcgaagagc agaccaaagt
     4741 ggctgatgca ttcggcgaag cgctgagcga agccctagac cgactacttc cagaaggcga
     4801 gaatatcatc gctgtgatgg gaggaactac tatggctgtg gtagccgaac aattgtctaa
     4861 tctggaaaat aagaaacggc ataacctgtt tgtccctgca agaggcggga ttggtgaagc
     4921 gataactgta caggcaaatt ctgtcagtgc aagaatggct gctaaagcaa acggtaacca
     4981 ccgtgcactt tatgttccgg aacaattaag tctggcaact tacaattctt tactcaatga
     5041 gccatctatc caagaagtct taaacttgat tagtgaagcg aattgtgtta ttcatagtat
     5101 tgggcgtgca ttacacatgg cagcacgtcg taagatgact gaaaaagaat tagtcatgct
     5161 gaaacaagca aatgctgttg cggaatcttt tggctacttt ttcaatgaaa aaggtgaagt
     5221 cgtttataag gtccctagaa tcggcataga attgcaagat ttggaaaaag tcccgatcat
     5281 catggcaatc gcgggcggaa aatcaaaagc caaagcaatt cgagcttata tgaaaaatgc
     5341 accaaaacaa acgtggctca tcactgatga agctgctgca aatgagattt taaaaggggt
     5401 aaccctttaa aataaatatt tttttgattt tcataaggag gaaatcttaa atgacagtta
     5461 aagtaggtat taatggtttt ggacgtattg gacgtctagc attccgtcgt atccaagatg
     5521 tagatggaat cgaagtagta gcaatcaacg acttgacaga tgctaaaatg ttggcacact
     5581 tgttaaaata cgacacaact caaggacgtt tcaacggaac agttgaagtt catgaaggtt
     5641 cattcaacgt aaacggaaaa gaagttaaag tattagctaa ccgcaaccca gaagaattac
     5701 catggggcga actaggcgta gatatcgttc tagaatgtac tggtttcttc acttctaaat
     5761 cagcagctga aaaacattta acagctggtg ctaaacgcgt tgttatctct gctcctggcg
     5821 gaaacgatgt tccaacaatc gtttataaca caaaccacga aacattaact ggtaaagaaa
     5881 cagttatctc aggtgcttca tgtactacta actgtttagc tcctatggct aaaacattga
     5941 acgacaaatt tggtgttgtt gaaggattaa tgacaactat ccacgcttac acaggtgacc
     6001 aaatgactct agacggacct catcctaaag gtgacttccg ccgtgcacgc gctgctgctg
     6061 caaacatcgt tcctaactca acaggtgctg ctaaagctat cggtttagta attcctgaat
     6121 tgaacggtaa attagacgga gctgctcaac gtgttcctgt accaacaggt tcattaacag
     6181 aattagtaac agttcttgaa aaagaagtaa ctgttgacga aatcaatgca gcaatgaaag
     6241 aagcttcaaa cgaatcttac ggatacaaca cagacgaaat cgtttcttca gatatcgttg
     6301 gtatgactta cggttcatta tttgatgcta cacaaactaa agtaatgaca gttggcgaca
     6361 aacaattagt taaaactgtt gcttggtacg acaacgaaat gtcatacact gcacaattag
     6421 tacgtacttt agaatacttc gctaacttat aagatagaca aacgtacaag tgtaaattat
     6481 agacatgtac atttaaaagc ggggaagcaa tcgcgcttct tccgcttttt ttgataataa
     6541 gtttattcag gaggaaataa aatggctaag aaaacagtga aagatatcga tttaaaagac
     6601 aaaaaagttc ttgtccgtgt tgacttcaac gttcctttga aagacggcgt gatcactgac
     6661 gacactcgta tcaaagcagc attgccaaca atcaactacg ttctagaaca aggcggaaaa
     6721 gcaatccttt tctctcacct tggacgtgta aaaacagaag aagataaaga aggaaaatct
     6781 ttagctccag ttgcaaaacg tctaggcgaa ttattaggta aagaagtaac attcgtacct
     6841 gaaactcgcg gagaacaatt agaagaagca atccgcaaca tgaatgacgg cgacgttgtc
     6901 gtattcgaaa atacacgatt tgaagatatt gacggtaaaa aagaaagcaa aaatgataca
     6961 gaactaggta aatactgggc ttcattaggc gatgtatttg ttaacgatgc tttcggtaca
     7021 gctcaccgtg cacatgcttc taacgtaggt atcgcttcaa ctggtatccc aacagttgct
     7081 ggattcttga tggaaaaaga aatcaagttc atcggtgaag cagtagaaga accaaaacga
     7141 ccaatgattg caatccttgg tggcgcaaaa gtttctgata aaatcggcgt aatcgaaaac
     7201 ttgattccta aagcagataa aatcttgatc ggtggcggaa tgacttacac attctacgaa
     7261 gcaaaaggaa tcaagatcgg taactctcta gtagaagctg ataaagtgga actagcaaaa
     7321 gaattaatcg aaaaagcagg cgacaaactt gtcttgccaa tcgacaatgt ctgtgcacca
     7381 gaattttcaa atgatgttga aacacaagta atcgaaggag acattcctga tggtttgatg
     7441 gctttagaca tcggcccagc atcagtaaaa ttatttgctg atacattaca aggtgcaaaa
     7501 acagttgttt ggaacggacc tatgggtgta tttgaaatga gtaactttgc taaaggtaca
     7561 atcggtgttt gtgaagcaat cgctaactta gaaggcgcaa caacaatcat cggtggcgga
     7621 gactcagccg cagcagcaga acaattagga tttgctgaca aattcacaca catctcaaca
     7681 ggtggcggcg caagcttgga attgcttgaa ggtaaagaat taccaggact agcagcaatc
     7741 aacgataaat aaacgaccaa gtttgctcaa ttctgacgag taatcgatca aaaggtgaag
     7801 ggagttttca tgactcccta gccatttttc aataaattaa ggagtgtgtt cctcaatgcg
     7861 taaacctatt atcgcaggta actggaaaat gaacaaaact gcatcagaag caaaagaatt
     7921 tgctgaagca gtaaaaaaca aaatcccttc aaatgacaaa gttgattcag tcatcggatc
     7981 tcctgcatta ttcttacaag aattaatgac atcagctgaa ggtacagaac taaaaatctc
     8041 agcacaaaac tgctactggg aaaactcagg tgcgttcact ggtgaaactt caccagcagc
     8101 tcttgctgat ctaggtgttc attatgtgat catcggacat tcagaacgtc gtgaatattt
     8161 ccatgaaaca gacgaagaca tcaataaaaa agcgaaagct atctttgcaa atggtatgac
     8221 accaatccta tgctgtggcg aatctttaga aacctatgaa gcaggcaaaa ctgctgaatg
     8281 gatcgaagga caaattacag ctggtttagc tgatctttca gctgaacaag tgtcaaacat
     8341 ggttattgct tatgaaccaa tctgggcaat cggtactggt aaatcagctg acgcacaaat
     8401 cgctgacgaa atttgtggag ttgtacgtca aacagttgaa aaactttatg gaaaagaagt
     8461 atcagaagca gttcgtatcc aatacggcgg ttctgtaaaa ccagaaaata ttgctgaata
     8521 catggcaaaa gaaaatgttg acggtgcttt agtcggtggc gcaagcttgc aagcagattc
     8581 attcttgtca ttgttagacg ctgtaaaata attgttgaaa atttgcgaaa aatgcaagaa
     8641 atgtttgcac taatgagcaa aattaactaa aatcgtatat gagggaaatc ccttaggaaa
     8701 aaacaaactc aaaggagaga caaaacatgt caattattac tgatgtttac gctcgcgaga
     8761 tcttagactc acgtggtaac ccaacaatcg aagtagaagt atacacagaa agcggagctt
     8821 ttggccgcgg aatggttcct tcaggtgctt caactggtga atacgaagca gttgaattac
     8881 gtgacggaga caaagctcgt tatggcggta aaggtgtaac taaagctgtc gacaacgtaa
     8941 acaacatcat tgctgaagca atcattggct acgatgttcg tgaccaaatg gctatcgaca
     9001 aagctatgat cgctttagac ggaactccta acaaaggtaa attaggtgcg aacgctattt
     9061 taggtgtttc tatcgctgtt gcacgtgcag ctgctgacta cctagaagta cctttatacc
     9121 actacctagg cggattcaac acaaaagtat tgccaacacc aatgatgaac atcatcaacg
     9181 gcggatcaca tgctgataac tcaatcgact tccaagaatt catgatcatg cctgttggcg
     9241 ctccaacatt caaagaagct ctacgtatgg gtgctgaagt attccacgca ttagcttcaa
     9301 tcttgaaagc tcgcggatta gcaacttctg ttggtgacga aggtggattt gctccaaacc
     9361 ttggttcaaa cgaagaaggt tttgaagtaa tcatcgaagc aatcgaaaaa gctggctatg
     9421 tacctggtaa agacgttgtt cttgctatgg atgctgcttc ttcagaattc tacgacaaag
     9481 aaaaaggtgt ttacgtacta gctgattcag gcgaaggcga aaaaacaact gacgaaatga
     9541 tcaaattcta cgaagaatta gtttctaaat atccaatcat ctcaatcgaa gacggattag
     9601 acgaaaacga ctgggacgga ttcaagaaat taactgacgt attaggcgac aaagttcaat
     9661 tagttggtga cgacttgttc gtaacaaaca ctcaaaaatt atctgaaggt atcgaaaaag
     9721 gaattgctaa ctcaatcctt atcaaagtga accaaatcgg tacattaaca gaaactttcg
     9781 aagctatcga aatggctaaa gaagctggct acacagcagt tgtatctcac cgttctggtg
     9841 aaacagaaga ttcaacaatc tctgatatcg ctgttgcaac aaacgctggc caaatcaaaa
     9901 ctggttctct atcacgtact gaccgtattg ctaaatacaa ccaattacta cgtatcgaag
     9961 accaacttgg tgaagttgca gaatacaaag gtttgaaatc tttctacaac ttaaaaaaca
    10021 aataattctt ttctaatgaa gatacatgat cgactattga aagatagtcg agtatgatca
    10081 cttagttaag taatcgaact ctctacttgc tgaaatatcg gctctttgtc aataaggact
    10141 gatgagttgt gcaaaatcaa atctgagtca gaatgaacca cattctggct cag
//
LOCUS       DARBET010000084        10086 bp    DNA     linear   BCT 07-NOV-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4027
            SAMEA114569841-rid20112183.denovo.086, whole genome shotgun
            sequence.
ACCESSION   DARBET010000084 DARBET010000000
VERSION     DARBET010000084.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA114569841
            Sequence Read Archive: ERR12203666
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 10086)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10086)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001972618.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001972618.1
            Genome Coverage       :: 132x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/07/2023 13:18:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,041
            CDSs (total)                      :: 2,975
            Genes (coding)                    :: 2,908
            CDSs (with protein)               :: 2,908
            Genes (RNA)                       :: 66
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 67
            CDSs (without protein)            :: 67
            Pseudo Genes (ambiguous residues) :: 0 of 67
            Pseudo Genes (frameshifted)       :: 26 of 67
            Pseudo Genes (incomplete)         :: 28 of 67
            Pseudo Genes (internal stop)      :: 20 of 67
            Pseudo Genes (multiple problems)  :: 6 of 67
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10086
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA114569841-rid20112183.denovo.086"
                     /isolate="VRE4027"
                     /db_xref="taxon:1352"
                     /country="Denmark"
     gene            complement(247..759)
                     /locus_tag="SCJ86_002396"
     CDS             complement(247..759)
                     /locus_tag="SCJ86_002396"
                     /EC_number="2.4.2.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295813.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenine phosphoribosyltransferase"
                     /protein_id="HEG1169915.1"
                     /translation="MDLKDYIASIPDYPSKGIVFRDISPLMADGDAYREATKQIVNYA
                     KEKRIDMVVGPEARGFIVGCPVAYELGVGFAPVRKKGKLPRETIEVTYGLEYGTDTLT
                     LHKDAIKPGQRVLICDDLLATGGTIKATIELIEQLGGVVVGCAFLIELMDLHGRDKIK
                     GYDIITLMEY"
     gene            complement(773..3070)
                     /gene="recJ"
                     /locus_tag="SCJ86_002397"
     CDS             complement(773..3070)
                     /gene="recJ"
                     /locus_tag="SCJ86_002397"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002316233.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded-DNA-specific exonuclease RecJ"
                     /protein_id="HEG1169916.1"
                     /translation="MRQANFDWKLSETEPSESFLQMIRELKLSPFVGKLLWQRGYHEE
                     KDINRFLHPKEQELHDPYLIHDMDKAISRIQEAVISGEKILVYGDYDADGITSATVMK
                     ETLELLGAEVEVFLPNRFEHGYGPNQSVYQEKIEEGIQLIITVDNGVAGNDAVAYAQQ
                     AGVDVIITDHHELPENLPDAYAIVHPRHPAGHYPFPDLAGVGVAFKVASALLEEPPAE
                     FLDLVAIGTIADLVSLTDENRILVSLGIDAIHHSERIGLQALFEESGVKMRDADETTI
                     GFSIAPRLNAIGRMGDPRPAVSLLATFDEEEASVQAKKLNEINEERKAIVEQITQEAL
                     AMVNKENHIHLLVNRGWHEGVLGIVAGKIMNETGKPTLVLTLKEDGSAKGSGRSIEAL
                     NLFEMLDQMRDLFTYFGGHHAAVGLTMPSENVTILQEKMNQYIVDHQIDLMRGPELRI
                     DEVLLPNEVTVERIDELKLLAPFGTDNPLPQFLFRQVQAENIKRIGANQQHLKFVLSD
                     ASSQLDAVAFGFGTQEEELLNNQVDVVGKLSINEWNGRKKPQLMVSDFSVGGLQVFDW
                     RAKRFREQSRTSEGSLYLAFDSSTLKFVPEIIQEAAIVFEGLDSVKAIIEKRKPNSLV
                     VMDCPDDLADLKEVFRSYSFNRVYLMGISPDEAYLNGVGTREQYAKLFKLIHSQERID
                     IRHKIKAIAQYLRIPEKLLIFMIQVFFELKFVTIENGVLQKVASPESHPLTESRLYQR
                     RLNKIKVEEFLLLSDIPTIKKWLTT"
     gene            complement(3212..3655)
                     /locus_tag="SCJ86_002398"
     CDS             complement(3212..3655)
                     /locus_tag="SCJ86_002398"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303793.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1049 domain-containing protein"
                     /protein_id="HEG1169917.1"
                     /translation="MKELKKQSNVILGFVLVLIIVLFAVLNNKNVPVSFGFTSFSAPL
                     ILVIIGSALIGALIVFLTASATLWQQKKQIKQLTQEMSEYQTELQKKIEEAKEEQQRE
                     FRNERAELEAAYQAELQAKTAQISQLKAPEEQKTQSPKQSFNYFD"
     gene            complement(3656..4444)
                     /locus_tag="SCJ86_002399"
     CDS             complement(3656..4444)
                     /locus_tag="SCJ86_002399"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313805.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HEG1169918.1"
                     /translation="MNLEDKVVLVTGSSGGLGAQICYEAAKQGAIVISCARRMAFVEG
                     VRDECRRLSGKEAYAFKVDVSNPESVDELYEKVMEEVGRVDILVNNAGFGIFEDFLTF
                     DLGKAYDMFEVNILGMMVLTQKFAIDMAERRQGHIINIASMAGKMATAKSTVYSATKF
                     AVLGFSNALRLELKPLGVAVTTVNPGPIETNFFDKADPSGSYLEKVGQIVLEPAKLAK
                     MIVRNMRHPKREINQPFILEVASKFYTLFPTIGDYLASGIFNKK"
     gene            complement(4441..5382)
                     /locus_tag="SCJ86_002400"
     CDS             complement(4441..5382)
                     /locus_tag="SCJ86_002400"
                     /EC_number="3.1.26.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376914.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease Z"
                     /protein_id="HEG1169919.1"
                     /translation="MELQFLGTGAGVPAKHRNVTSIALKLLDERNEVWLFDCGEGTQM
                     QILRTTIRPRKIGKIFITHLHGDHIFGLPGLISSRSFQGGDTPLEIYGPKGIEEYIKV
                     SLGISQTRLSYPLKFIELNETAPIFTDQQFSVYTKKLNHGIDSFGYRVVEHDHKGELQ
                     VDRLKELGIPAGPLYGKLKQGETIQLEDGRTINGKDFVGPDKKGRIVTILGDTRKTHN
                     SVVLAENSDILVHESTFNKDEARMAHNYFHSTTHQAAEVAKEAQAKRLLLTHISARYL
                     GKAALELEKEAQEVFENTTIMKDFDSIEIPFREEEEA"
     gene            complement(5559..6146)
                     /locus_tag="SCJ86_002401"
     CDS             complement(5559..6146)
                     /locus_tag="SCJ86_002401"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336978.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1269 domain-containing protein"
                     /protein_id="HEG1169920.1"
                     /translation="MSKRVIILNFQVESQAYQAFSEIKKLHAVRSIKGEQMAVVSHDP
                     NGTHQFKIEDFIDFTGNNKSSTGGLIGMLVGVLGGPLGILLGWFAGGMIGATQDAKEI
                     KDAQTVFDFVGNKIGEGATGLILIADEEDNRPLNQLVMMELGGEITRLDYEDVEKDVH
                     DAKEAENHAKEAAQKTWKDKHPQTSNDNESSGSSN"
     gene            complement(6367..7674)
                     /gene="obgE"
                     /locus_tag="SCJ86_002402"
     CDS             complement(6367..7674)
                     /gene="obgE"
                     /locus_tag="SCJ86_002402"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306800.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase ObgE"
                     /protein_id="HEG1169921.1"
                     /translation="MFLDQVTIDVKAGKGGDGMVAFRREKYVPDGGPAGGDGGRGGDV
                     ILIVDEGLRTLMDFRFNRHFKAQPGENGMSKGMHGRGSEDTYVKVPQGTTVRDAETGA
                     LLGDLIENGQTLVVAKGGRGGRGNIRFASPRNPAPEIAENGEPGQERKIELELKVLAD
                     VGLVGFPSVGKSTLLSVISSARPKIGAYHFTTLVPNLGMVTTSDGRSFAAADLPGLIE
                     GASQGVGLGTQFLRHIERTRVILHVIDMSGMEGRDPYEDYLAINKELSTYNLRLLERP
                     QIIVANKMDMPDAPENLVKFKEQLNKEKEDEFADDIPVFPISGVTRQGLDALLNATAD
                     LLEVTPEFPLYEEELEEETVHYGFNPEGPEFQIDRDSDATWILSGEKIEKLFQMTNFD
                     HDETVMRFARQLRGMGVDEALRARGAKDGDLVRIGEFEFEFVE"
     gene            7968..8978
                     /gene="gap"
                     /locus_tag="SCJ86_002403"
     CDS             7968..8978
                     /gene="gap"
                     /locus_tag="SCJ86_002403"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005877559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I glyceraldehyde-3-phosphate dehydrogenase"
                     /protein_id="HEG1169922.1"
                     /translation="MTVKVGINGFGRIGRLAFRRIKEVSDDIEVVAINDLTSPTMLAQ
                     LLQFDSTHGTYPGTVTATEDSIVVDGEATRVYAEPDASKIPWAKENGVDIVLECTGFY
                     TSEEKAKAHLDAGVKRVVISAPAGAMKTIVYNVNDDTLDENDKIISAGSCTTNCLAPM
                     AYFLNNEFGIEVGTMTTVHAYTSTQMLLDGPVKGGNLRAARSAADNTIPHSTGAAKAI
                     GLVIPELQGKLQGHAQRVPVVDGSLTELVSVLKTKVTADQVNEAIKKHTVDNPSFGYD
                     DRQIVSSDVIGTTQGSIFDPTQTEVTTAGDFQLVKTVAWYDNEYGFTCQMIRLLEKFA
                     NL"
     gene            complement(9063..9998)
                     /locus_tag="SCJ86_002404"
     CDS             complement(9063..9998)
                     /locus_tag="SCJ86_002404"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002372379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HEG1169923.1"
                     /translation="MKLWMKILLGVALALIVALVFAGNYFYNYAVVPSEKDFLDEDTP
                     GTTQTSKETDAHKWFSSKENRSDWTITSKDGLKLSAIYLPAETKSEKTALVAHGYMGN
                     AETMTNYAKMFHDMGYNVLVPDARGHGKSEGDYIGFGWPERKDYVQWINKVLENNGKS
                     QEIVLYGVSMGAATVMMTSGEKLPDNVKAIIEDCGYSSVHDELAYQLDDMFSLPAFPL
                     MQVTSLVTKVRAGYFFGEANAVEQLKKNQRPMLFIHGDEDTFVPFEMLDKVYRATKGP
                     KEKYVVKGAEHAEAYKTDPEKYQQVVQQFLSQYVH"
BASE COUNT         2778 a         2298 c         1746 g         3264 t
ORIGIN      
        1 ttttacgggg gtgtgacaaa aatcggtcac atcctttttt tcgaataaac ggtgttctaa
       61 agccagtttt tcggtattaa gtcaaattgc ggaaaaagtg attttttaaa cttctactgg
      121 tctttgcttg tttccattta taaaaaaata gcgaccctca aaagtcagat agagaatctg
      181 acttttgagg gtcgctttaa aatcgcttct ttaagaataa catcaagaat agcttctttt
      241 ttctgcttaa tattccatta atgtaatgat gtcatatcct ttgattttgt cgcgtccatg
      301 gagatccatc aattcgatca aaaatgcgca gcctacgact acaccgccta attgttcgat
      361 cagttcgatc gtcgctttga ttgttcctcc agtagctagc aaatcgtcac aaatcagcac
      421 acgttgacct ggtttaatgg catccttatg caatgtcaat gtatctgttc catattctaa
      481 tccataagtc acttcgatgg tttctcgagg taacttccct tttttgcgga ctggggcaaa
      541 accgactcct aactcataag caacaggaca tcctacgatg aatccacggg cttctggccc
      601 aactaccata tcaattcgtt tttcttttgc ataatttacg atttgtttag tagcttcccg
      661 ataagcgtca ccatctgcca tcagtgggga aatatctcga aacacaattc cttttgacgg
      721 atagtcggga atactggcaa tataatcttt taaatccact ctttgtttcc tcctaggttg
      781 ttaaccattt ctttatggta ggaatatcgc tcaaaagcaa aaattcctca actttgattt
      841 tattgagccg tcgctggtat aatctgctct cagtcaaagg atgactttct gggctagcga
      901 ctttttgtaa aacaccgttc tctattgtaa caaatttcaa ttcaaaaaac acctgtatca
      961 taaaaattaa taatttttca gggatacgca aatattgagc tattgctttt attttatgac
     1021 gtatatcaat tcgttcttga gaatggatca atttaaacaa ttttgcgtac tgttctcttg
     1081 tgcctacccc attcaaatag gcctcatcag gtgaaatccc catcagataa acacgattga
     1141 atgaataaga ccgaaaaact tctttcagat ccgctaaatc atctggacaa tccataacaa
     1201 ctagagagtt tggttttctt ttttcaatta ttgcctttac agaatctagt ccttcgaaaa
     1261 caatcgcagc ttcctggata atctcaggca cgaatttcaa cgtcgaagaa tcaaatgcta
     1321 aataaaggct accttccgac gttcggctct gttcacggaa acgttttgcc cgccaatcaa
     1381 atacttgcag accacccaca gaaaaatcag ataccatcag ctgcggtttc ttacgcccgt
     1441 tccattcatt gatcgaaagc tttcctacca catctacctg attattcagc agttcttctt
     1501 cttgagtacc aaaaccaaaa gcgaccgcat caagctgact cgatgcatcg gaaaggacaa
     1561 atttcagatg ctgttggttt gctccgattc ttttgatatt ttctgcttgg acttgtcgaa
     1621 ataaaaactg aggcaaagga ttatccgtac caaatggcgc caatagtttc agctcatcaa
     1681 ttcgctctac tgttacttca tttggcagca atacttcatc aattctcaac tcaggccctc
     1741 tcatcaagtc aatttgatga tcgactatat attggttcat cttctcctgt agtatagtga
     1801 cattttcact tggcatcgtt aaaccgactg ctgcatgatg tcctccgaag taagtaaata
     1861 aatctcgcat ctggtcaagc atttcaaaaa gattcaaggc ttcaatacta cgtccagatc
     1921 ctttagctga tccatcttct tttaaagtaa ggacaagcgt tggtttaccc gtttcattca
     1981 ttattttccc agcaacaatg cccaaaacgc cttcatgcca tcctcggttt accaatagat
     2041 ggatatgatt ctccttattg accatcgcta atgcttcttg tgttatctgc tctacgattg
     2101 cttttctttc ttcattgatc tcattcaatt tttttgcttg aacggatgct tcctcttcat
     2161 caaacgtcgc aagtaacgag acggcaggac gtggatcgcc catccgaccg attgcattta
     2221 gtctgggagc aatcgaaaaa ccgatcgttg tttcgtctgc gtccctcatt tttacgccgc
     2281 tttcttcaaa tagcgcctga agtcctatcc gttctgaatg atgaatggca tcgattccta
     2341 atgaaacaag gattcgattc tcatcagtca atgaaactaa atcggcgatc gttcctatcg
     2401 ctactaaatc caaaaattct gcaggcggtt cttccaataa tgcagaagct actttgaatg
     2461 ctactccgac tcctgccaaa tctggaaatg gatagtgccc cgctggatga cgagggtgaa
     2521 caatcgcata agcgtctggt aaattctcag gtagttcgtg atgatctgta atgatcacgt
     2581 ctacaccagc ttgctgagcg tatgctaccg catcatttcc tgctaccccg ttatcaacag
     2641 tgatgatcaa ctggattcct tcttcgatct tttcttggta taccgattgg tttggtccat
     2701 acccatgctc aaatcgattt ggcaggaata cttctacttc tgcaccaagt aattccagtg
     2761 tttctttcat taccgttgca ctggtgatcc catctgcgtc ataatcaccg taaacgagga
     2821 ttttttctcc actgattaca gcttcttgga ttcggctgat tgctttatcc atatcgtgta
     2881 tcagatacgg atcatgtaat tcttgttcct ttggatgtaa aaatcggttg atatcctttt
     2941 cctcatggta acctctttgc cacagtaact taccgacaaa tggagataat ttcagttcac
     3001 ggatcatttg gagaaaggat tcagaaggct ctgtctcgct taacttccaa tcaaaatttg
     3061 cttgtctcac tttctcactc ctaacttagt tattatagca aaggaaatca gcaaaaaaaa
     3121 gcagaaagaa agaataaaaa cagatcagat ccgctagaca gatgttcagc aggacctgat
     3181 cttttgtacg accatttatg aaatatccct attagtcaaa ataattgaag ctttgtttgg
     3241 gcgattgagt tttttgttct tccggcgctt tcaattgact gatttgtgcg gttttcgctt
     3301 gtaactccgc ctgataagca gcttccaact ccgcacgttc gtttctaaac tcgcgttgct
     3361 gttcttcttt tgcttcttca attttctttt gaagttctgt ttgatattct gacatttcct
     3421 gagtcagctg tttgatttgc tttttttgct gccatagtgt tgccgaagca gtcaaaaata
     3481 caatcagtgc accaatcagc gcagaaccga tgatcactaa gatcaatggc gcagaaaagg
     3541 aagtgaagcc aaaacttaca ggaacatttt tattgttcaa tacagcaaaa agaacaataa
     3601 tcaaaactaa aacaaatcca agaatcacat tgctttgctt tttcaattcc ttcacctact
     3661 ttttgttaaa aattccgctg gctaaataat caccaatcgt tggaaacagc gtataaaatt
     3721 ttgatgcaac ttctaaaata aacggttgat tgatttcccg ttttggatgt ctcatattgc
     3781 ggacgatcat ttttgccaac ttggcaggtt ctaaaacgat ttgtccgact ttttccaaat
     3841 agcttccgct tggatcagcc ttatcgaaga aatttgtctc gattggacct ggattcactg
     3901 tcgtaacagc tacgccaagt ggtttcaatt ctaatcgcaa agcgttggag aagccaagga
     3961 ctgcaaattt cgtggcagaa taaaccgttg attttgcagt tgccattttt ccagccatag
     4021 aggcgatatt aatgatatga ccttgtcttc tttctgccat atcgatcgca aatttttgtg
     4081 tcaaaaccat cattcctaag atgttgactt caaacatatc gtatgctttt ccaaggtcga
     4141 atgtaaggaa gtcttcgaaa atcccaaaac ccgcattgtt taccagaata tcgacgcgcc
     4201 cgacttcttc catcaccttt tcatataatt cgtccacgct ttcaggattg ctgacgtcta
     4261 ccttgaaagc ataagcctct ttgccgctca agcgtctgca ctcgtctctg acaccttcta
     4321 caaatgccat tcttcgtgcg caacttataa cgattgctcc ttgctttgct gcttcataac
     4381 agatttgtgc gcccagaccg cctgaactac ctgttactaa aacaacttta tcttctaaat
     4441 tcatgcctct tcctcctcgc gaaatggaat ctcaatgcta tcaaaatctt tcataatcgt
     4501 cgtgttttca aacacttctt gtgcttcttt ttcaagctct aaagctgctt ttcctaaata
     4561 tctcgcactg atatgagtaa gtagcagccg ttttgcctgc gcttcttttg caacttcagc
     4621 agcttgatga gtcgtggaat ggaaatagtt gtgcgccatt cttgcttcat ctttattaaa
     4681 tgtgctttca tgtaccagaa tatcgctatt ttccgcaagc acaacggaat tatgtgtttt
     4741 tctcgtatcg cctaaaatag tcacgattcg tcctttttta tctggaccta cgaaatcttt
     4801 tccattgatc gtccgcccat cttctagttg gatcgtttca ccttgcttca gtttgccgta
     4861 cagtggtcca gctggaatac ctaattcttt tagacgatcc acctgcaatt ctcctttatg
     4921 atcatgttca accacacgat aaccaaaact gtcgatccca tgatttagtt tttttgtata
     4981 aacagaaaat tgctgatcag taaaaatcgg ggctgtttca tttagctcaa tgaatttcag
     5041 cggataactt aaacgcgttt gcgaaatacc taaagagact tttatgtatt cctctatccc
     5101 ttttggcccg taaatctcta atggcgtatc cccaccttga aatgaccggc tgctgatgag
     5161 cccgggaagt ccaaaaatat gatcgccgtg taaatgtgta atgaaaattt taccgatttt
     5221 tctcggacga atagtcgttc gcaaaatctg catttgcgtg ccttcgccac agtcgaacaa
     5281 ccaaacttca ttgcgctcgt ctaaaagttt caatgctata cttgtcacat ttctatgttt
     5341 tgcaggaaca cctgcacctg ttcctaaaaa ttgtaattcc ataatctacc tgactttctt
     5401 taagttcctc tttaatttta gggtaaaaag aagggataat caaccgtaag tcgagaatgg
     5461 gttcaaatta aaaataacgt tctaaagcga tagcgttttc tttgaaaatt actataaaaa
     5521 cagcagcgaa cccgtttgag atcctctgct gttttttatt aatttgaaga tccagaactt
     5581 tcattgtcgt tggaagtttg cggatgtttg tccttccaag tcttttgtgc agcttctttc
     5641 gcatgatttt cagcttcttt cgcatcatgc acatcttttt cgacatcttc ataatccaag
     5701 cgagtaattt ctccgcctaa ttccatcatt accaattgat tcagtgggcg attgtcctct
     5761 tcatcagcaa tcaagatcag tcctgtagct ccttcaccga ttttatttcc tacaaagtcg
     5821 aacacggtct gggcatcttt tatttctttg gcatcttgtg tcgcaccgat cattcctccg
     5881 gcaaaccatc ctaataaaat ccctagcgga ccacctaata caccaaccaa cattccgatt
     5941 aatccacccg tagaagattt gttattaccg gtaaagtcga taaaatcttc tattttgaat
     6001 tgatgtgtcc cattaggatc atggctgaca actgccatct gttctccttt aatcgaacga
     6061 acagcatgta actttttgat ttctgaaaat gcctgatatg cctggctttc tacttgaaaa
     6121 tttaaaatga ttactcgttt gctcattaaa aacgcccctt tcataccttc ttgttattta
     6181 ttttaccttt aattgaaaaa taagaaaaga aaagcactct ttttttccaa taaaataggg
     6241 ctgtgacaaa agtcgtatca cagctccttt ttccgaataa acggcgttct tgaagctgat
     6301 cacttctgtc acaacctccc ttaaatctga atctatattt tttggtattt gtttttattt
     6361 gcttgcctat tcgacaaatt caaattcaaa ctcaccgata cgtacaaggt cgccatcttt
     6421 cgcaccgcgt gcccgaagag cttcatccac acccatacca cgtaattgac gagcaaaacg
     6481 catcactgtt tcatcatgat cgaaattcgt catttggaat aatttttcga tcttctcacc
     6541 agataagatc cacgttgcat ctgaatcacg atcgatttgg aactcaggac cttctggatt
     6601 gaatccatag tgtacagttt cttcttccag ttcttcttcg tacaatggga attctggcgt
     6661 tacttctaac aaatctgctg tagcattcaa taacgcgtcc aatccttgtc gagtcactcc
     6721 agaaattgga aatactggga tgtcatcagc aaattcatct tctttttcct tgttcagctg
     6781 ttctttgaat ttcactagat tttccggtgc atccggcata tccattttgt ttgccacgat
     6841 gatttgcgga cgctctagca agcgtaagtt ataagtagat aattctttgt tgatggcaag
     6901 atagtcttca taaggatctc gtccttccat cccactcata tcgatcacat gtaaaattac
     6961 tcgtgttctt tcgatatgac gaaggaattg tgtacccagc ccaacacctt gcgaagctcc
     7021 ttcaatcaag cctggcaagt ctgctgcggc aaaacttctg ccatcacttg tagtaaccat
     7081 tcctagatta ggtacgagcg tcgtgaaatg ataagcgccg atttttggac gagcagatga
     7141 aataacggat agtaaagtcg atttcccgac agatggaaaa cctacaagtc caacgtctgc
     7201 caatactttc aattctagtt cgatttttcg ctcttgtcct ggttcaccgt tttcagcaat
     7261 ttccggtgca ggattccgag gagaggcaaa acggatattc cctcgtccac cgcgtccacc
     7321 ttttgccaca actagtgttt gaccattttc aattaaatct cctaaaagag caccagtctc
     7381 cgcatctcgg acagtggttc cttgtggtac tttaacatag gtatcttctg aaccacgacc
     7441 gtgcatccct ttactcatcc cattttcacc tggctgcgct ttgaaatggc gattgaaccg
     7501 aaaatccatc aatgtacgta acccttcatc gacgatcaag atgacatcgc cgccacgacc
     7561 accgtctccg ccagccggtc cgccgtcagg aacgtatttt tcgcgacgaa atgcaaccat
     7621 tccgtctccg cccttgccag ctttcacatc gatcgttact tgatctaaaa acatggacat
     7681 aattaagtcc tcctattatt catttattta tttgactgcc tgaatttcca caataaacaa
     7741 aagtcttact tattttatcg gtgaaatctg attttgtcta tcatttcagc aaaaagattg
     7801 tttaccttct tcttttttta tcatttggta aggtttccaa ccttttttct gctaccaagt
     7861 ataatttttt gacaaaatat tctttgcgta gcagaataac agtgtattaa ttgtattaat
     7921 tgaggatgcc gtttcacagc acccctaata aaggaggaac ttttgatatg acagtaaaag
     7981 taggtattaa cggatttggg cgtattggcc gtctggcttt ccgccgcatc aaagaagtct
     8041 cagatgatat tgaagtagta gcaatcaacg acttaacaag tccaactatg ttagcacaat
     8101 tattgcaatt tgactctact catggtactt atcctggcac agttactgca acagaagact
     8161 caatcgtagt ggatggcgaa gcaactcgtg tttatgcaga acccgacgca agcaaaatcc
     8221 cttgggcaaa agaaaacggt gtagatatcg tactagaatg tactggtttt tatacatctg
     8281 aagaaaaagc aaaagcgcat ttagatgctg gtgtcaaacg agtagttatt tctgccccag
     8341 ctggtgctat gaaaaccatc gtctataacg tcaatgatga tacattagat gaaaacgaca
     8401 aaatcatctc agcaggttca tgtacaacaa actgtttagc acctatggct tatttcttaa
     8461 ataatgaatt tgggatcgaa gttggtacaa tgacaacagt tcatgcctac acttccacac
     8521 aaatgctctt agatggacct gtaaaaggcg gtaatttgcg tgctgctcgt tcagctgctg
     8581 acaatactat cccacattct actggtgctg cgaaagcaat cggcttagtt atccctgagc
     8641 tacaaggaaa attacaagga cacgcacaac gtgttccagt cgttgatggt tcattaactg
     8701 aattggtttc agtattgaaa acaaaagtaa ctgctgatca agtaaacgaa gcaatcaaaa
     8761 aacacacagt tgacaatcct tcattcgggt atgatgaccg tcaaatcgtt tctagcgatg
     8821 tcattggaac aactcaaggt tcgatctttg acccaacgca aacagaagta acaactgctg
     8881 gtgatttcca acttgttaaa actgttgcat ggtatgacaa tgagtatgga tttacttgcc
     8941 aaatgattcg cttactagaa aaattcgcta acttgtaatt tgatagatca atcgataaaa
     9001 gaagcctttg actggatcca gtcaaaggct tctttctttt catcccctac ttttaaactc
     9061 gttcaatgaa catattggct taaaaattgt tggactactt gctgatattt ttcaggatct
     9121 gtcttgtatg cttccgcatg ctcggctcct tttacaacat acttttcttt tggtcccttc
     9181 gttgcacgat agaccttatc taacatttca aatggtacaa atgtatcttc gtctccatgt
     9241 ataaagagca ttgggcgctg gtttttcttc aattgttcca ctgcatttgc ttcaccgaaa
     9301 aagtagccag cacggacttt tgtcactaga ctcgttactt gcataagcgg aaatgctgga
     9361 agactaaaca tatcgtctag ctgataagca agctcatcat gaacggaaga ataaccgcaa
     9421 tcttcaatga ttgctttcac attatctggt agtttttctc cgctagtcat cataactgtt
     9481 gctgctccca tgctgacacc gtatagaacg atttcttgcg attttccatt gttttctagc
     9541 actttgttta tccactgtac atagtcttta cgctctggcc atccaaatcc aatgtaatcc
     9601 ccttcgcttt ttccatgtcc tcttgcatca ggaacaagga cattgtaacc catatcatga
     9661 aacatctttg cgtaattggt catcgtttca gcgtttccca tatagccatg agccacaaga
     9721 gctgtttttt cacttttcgt ttcagcagga agataaatcg ctgataattt cagaccgtct
     9781 tttgaagtga tggtccaatc gcttcggttt tcttttgaac taaaccattt atgtgcatcc
     9841 gtttctttgg atgtctgtgt tgtaccaggt gtatcttcgt ctaaaaagtc tttctcagaa
     9901 ggcacaacag cataattata aaaataattc cctgcaaaca ctaacgcaac aatcagagct
     9961 aaagcgacgc ctaaaaggat tttcatccaa agtttcataa atcatccatc tcatttctct
    10021 tttttcatat aaccttgaat tagtataatc aaagaatctc tactatgcaa tgttcatttt
    10081 tataac
//
LOCUS       DARBET010000085        10084 bp    DNA     linear   BCT 07-NOV-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4027
            SAMEA114569841-rid20112183.denovo.087, whole genome shotgun
            sequence.
ACCESSION   DARBET010000085 DARBET010000000
VERSION     DARBET010000085.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA114569841
            Sequence Read Archive: ERR12203666
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 10084)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10084)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001972618.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001972618.1
            Genome Coverage       :: 132x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/07/2023 13:18:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,041
            CDSs (total)                      :: 2,975
            Genes (coding)                    :: 2,908
            CDSs (with protein)               :: 2,908
            Genes (RNA)                       :: 66
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 67
            CDSs (without protein)            :: 67
            Pseudo Genes (ambiguous residues) :: 0 of 67
            Pseudo Genes (frameshifted)       :: 26 of 67
            Pseudo Genes (incomplete)         :: 28 of 67
            Pseudo Genes (internal stop)      :: 20 of 67
            Pseudo Genes (multiple problems)  :: 6 of 67
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10084
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA114569841-rid20112183.denovo.087"
                     /isolate="VRE4027"
                     /db_xref="taxon:1352"
                     /country="Denmark"
     gene            162..392
                     /locus_tag="SCJ86_002405"
     CDS             162..392
                     /locus_tag="SCJ86_002405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331563.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1146 domain-containing protein"
                     /protein_id="HEG1169924.1"
                     /translation="MQVYGIDAVLRIVSHLAFIYLAFWSLRSLRIETFFRPMHNTQIR
                     MAIVLFSIFLGYTASNFFLEVIALCRNLFVSL"
     gene            489..1790
                     /gene="murA"
                     /locus_tag="SCJ86_002406"
     CDS             489..1790
                     /gene="murA"
                     /locus_tag="SCJ86_002406"
                     /EC_number="2.5.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734894.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylglucosamine
                     1-carboxyvinyltransferase"
                     /protein_id="HEG1169925.1"
                     /translation="MEEIIVRGGNQLNGTVRIEGAKNAVLPILAASLLAEEGITTLDN
                     VPILSDVFTMNQVIRHLNVDVDFDEQKNQVTIDASRQLEIEAPYEYVSQMRASIVVMG
                     PLLARNGHAKVAMPGGCAIGKRPIDLHLKGFQALGAKIIQKNGYIEAIADELIGNTIY
                     LDFPSVGATQNIMMAAVKAKGTTIIENVAREPEIVDLANILNKMGAQVYGAGTETMRI
                     EGVDHLHAVNHSIVQDRIEAGTFMVAAAMTQGNVLIADAISEHNRPLISKLIEMGAEI
                     IEEEGGVRVIGPKHILPTDVKTMPHPGFPTDMQAQMTAIQLVAEGTSVVTETVFENRF
                     QHLEEMRRMNAHVKIDGNVAIMDGNHELQGAEVYATDLRAAAALVLAGLKANGITRVR
                     NLNYLDRGYYNFHIKLQQLGADVERVDMDQTSAEKTAQTIA"
     gene            1803..1979
                     /locus_tag="SCJ86_002407"
     CDS             1803..1979
                     /locus_tag="SCJ86_002407"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit beta"
                     /protein_id="HEG1169926.1"
                     /translation="MSNQRYILLTLIKILVVILLLILLFVAGTMIGYGVIGGGNPFKV
                     FQPSLWIHIRDFFH"
     gene            2114..2410
                     /gene="yidD"
                     /locus_tag="SCJ86_002408"
     CDS             2114..2410
                     /gene="yidD"
                     /locus_tag="SCJ86_002408"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338990.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane protein insertion efficiency factor
                     YidD"
                     /protein_id="HEG1169927.1"
                     /translation="MKKILIYLVRGYQRFISPAFPPSCRYYPTCSNYMIKAIQTHGAL
                     KGTVMGTARILRCHPFVKGGIDYVPLTFQLTRNKKDKKEPDYKKVVKNTSSTER"
     gene            2474..2941
                     /locus_tag="SCJ86_002409"
     CDS             2474..2941
                     /locus_tag="SCJ86_002409"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEG1169928.1"
                     /translation="MIEELNKKDLPWSLLLDADPDKEKVQVYVSRGSGWIWKEKEKTI
                     GVLIYVAREKEYEIVNVAVAPTYQGKGIGGKLLETAFQKLSQLASSQTQIIIRTGSTS
                     SAALHLYQKMGYVEIGRVKDYFIHHYSEPIFENGDQLRDQVILAIHLPKNSFN"
     gene            3026..3910
                     /locus_tag="SCJ86_002410"
     CDS             3026..3910
                     /locus_tag="SCJ86_002410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325063.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family protein"
                     /protein_id="HEG1169929.1"
                     /translation="MGILVFDFGGSAVKYGCWDGKEIKGKGQFATPESWEEMKAQLFQ
                     VYKKILVSFEVEGIGISSPGVVNNEKQVIEGISAIPYIHGFNIFRDLETLFQLPVTIE
                     NDANCAGMAEFYEGAARDYQDVAFVVAGTGIGGALFTKGQINKGAHLYGGEFGLMFLN
                     GDKTFSKLGTAVQMAWRYCERKGLDKNSYTGKDVFELAETGDAIAKEEVESFYTYLTK
                     GLFSIQFSFDPEVIVLGGGVSAKEGLIDEIKSRMKKLTEQFDLHDFEPQILLCEYRND
                     ANLVGAAANYLAVRQGEN"
     gene            4024..4260
                     /locus_tag="SCJ86_002411"
     CDS             4024..4260
                     /locus_tag="SCJ86_002411"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002316425.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2969 domain-containing protein"
                     /protein_id="HEG1169930.1"
                     /translation="MMSKKNKDVEVRIEEVKKDIKGKNYEVTQLFIGKKMIGEILAYG
                     PKEYEIFFGEEDFGKEKSLENAIETVIRHWNLHE"
     gene            4348..4428
                     /locus_tag="SCJ86_002412"
     tRNA            4348..4428
                     /locus_tag="SCJ86_002412"
                     /product="tRNA-Tyr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:4382..4384,aa:Tyr,seq:gta)
     gene            4435..4506
                     /locus_tag="SCJ86_002413"
     tRNA            4435..4506
                     /locus_tag="SCJ86_002413"
                     /product="tRNA-Gln"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:4467..4469,aa:Gln,seq:ttg)
     gene            4631..4801
                     /locus_tag="SCJ86_002414"
     CDS             4631..4801
                     /locus_tag="SCJ86_002414"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292784.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEG1169931.1"
                     /translation="MKWKEKVDEAAEKLYDLVKSEKYNIEVNIPKKGGKAVQVKSKRP
                     TNHTKKWMAKNR"
     gene            5021..5728
                     /locus_tag="SCJ86_002415"
     CDS             5021..5728
                     /locus_tag="SCJ86_002415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010737976.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HEG1169932.1"
                     /translation="MSNQLPVYIQIHDQLKNEIEQGIWKVGDRLPSERELAVKFNVSR
                     MTLRQAIQNLADEGILERKIGSGTYVAREKVQEKMSGATSFTEIMESQNRVPSSRTIS
                     YFLTSPSSSEMEKLNLSKDDTILRMERIRFADDVPICFEVASVPQKLIDGYSKAEITE
                     SLYRTLEEKGGKKIGPANQTITAVLASEKIADYLEVKRGDAVLRLRQISYFEDGTPFE
                     YVRTQYVGNRFEFYLEK"
     gene            complement(5788..6432)
                     /locus_tag="SCJ86_002416"
     CDS             complement(5788..6432)
                     /locus_tag="SCJ86_002416"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400550.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YigZ family protein"
                     /protein_id="HEG1169933.1"
                     /translation="MIERYRTIKENGQSEIEIKKSRFICSLRRIESEDQAKSFIQEIK
                     KEHWKANHNCSAFIIGEKNEIQRSSDDGEPSGTAGVPMLEVLKKNQLINVAAVVTRYF
                     GGTKLGTGGLIRAYTHAVSQALSDIGIVEGTLQQEISVMITYPQLGKLQNYLELQHFF
                     IKEIQYTDQVSVICAVDEKSADHFKESIIELLNGQVQFEEGPVSYHEQLVTDHI"
     gene            6559..6846
                     /locus_tag="SCJ86_002417"
     CDS             6559..6846
                     /locus_tag="SCJ86_002417"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294988.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEG1169934.1"
                     /translation="MQERMKKYDAITHYLKNNGGSQVTLTFTQFDELLFPSNGLPKTA
                     RESTDWWANDYKHPEKGAYGWINAGYEVVVINLDKEYVVFNKLVKSSWLFD"
     gene            6954..7274
                     /locus_tag="SCJ86_002418"
     CDS             6954..7274
                     /locus_tag="SCJ86_002418"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292778.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HEG1169935.1"
                     /translation="MAKKTIMLVCSAGMSTSLLVTKMQKAAEAKGIDSDIFAVSASDA
                     DNNLANKDVDVLLLGPQVRFMKADFEKRLEPKGIPLDVINMADYGMMNGEKVLDQAIT
                     LIGE"
     gene            7355..8668
                     /locus_tag="SCJ86_002419"
     CDS             7355..8668
                     /locus_tag="SCJ86_002419"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306206.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase family protein"
                     /protein_id="HEG1169936.1"
                     /translation="MNHFWGRRLLTRDIKAIDCEEVNLPNYEKIAAVERLGNWILCHR
                     CGMRTSVIDGQLADYGYFCTHCLYLGRCDTQQDLYLFDQPEAKPREVVFFWTGQLTEK
                     QTEIAKRILCHSEKRHHLIWAVTGAGKTEMLYPILVETLKAGGRVAICTPRVDVCNEL
                     FLRYRYVFPEEKITLLHGNTATAYTFTSFVICTIHQLYRFYRSFDLIVIDEIDAFPYN
                     GDAGLAHAVQTAIKPDGRFIYLSATPDKKLLKEIKQTFDIHKLALRFHRRLLPEPVLV
                     FMNNWRQKCKNKKKIKSLVRIIHQLIKENHILLFCPSITMMHRLKATLQEALPRYEIT
                     DVSSRDNQRAEKVQKMREAKYDILLTTMILERGVTFERISVIVLGADHPVFTKSSLVQ
                     IAGRADRKGPYTNSQVYFFYEEKTAAIRKACQEIKEMNEEGKKML"
     gene            8665..9384
                     /locus_tag="SCJ86_002420"
     CDS             8665..9384
                     /locus_tag="SCJ86_002420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ComF family protein"
                     /protein_id="HEG1169937.1"
                     /translation="MRCSCCSKDIVQNLTFSEIIGVSKYRLPQTCSVCRAQFHLLETR
                     GCSGCQKPIHFNLESTKKKVKSIPVYCSDCQEWKKKYPTYDFQHRAFFVYDENMKAWL
                     NQYKFMGDIRLAGTFAYKWTQIKKEYPTYIYCPIPLSEKRRTERGFNQVSEMLKAARI
                     PFQMLLKREKHLLAQAKNTRKERLEMPQPFELAVTFSKIQNKKILIIDDVYTTGQTLF
                     HAADCLQVAQPKIIRTISLAR"
     gene            9512..10063
                     /gene="raiA"
                     /locus_tag="SCJ86_002421"
     CDS             9512..10063
                     /gene="raiA"
                     /locus_tag="SCJ86_002421"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292771.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome-associated translation inhibitor RaiA"
                     /protein_id="HEG1169938.1"
                     /translation="MFRYNVRGENIEVTEAIRDYVEKKVGKLERYFSDAPDATAHVNL
                     KVYTEKTAKVEVTIPLPYLVLRAEETSPDLYASIDLVVDKLERQIRKFKTKINRKSRE
                     TGMNTADATALFNEEDENENDSELDIVRTKRLSLKPMDSEEAVLQMNMLGHNFFIFED
                     AETNGTSIVYRRKDGKYGLIETD"
BASE COUNT         3359 a         1668 c         2151 g         2906 t
ORIGIN      
        1 ttttgtctca gtcccttttt ttgcatattt ttagcattta tttcctaaaa aatgtaaggg
       61 gattcctata tttttaagta tacttgtttt tccttttata cattttatgg tattatcatt
      121 ttattagtta ttataaaaca atcgttagaa aggacgtcat tatgcaagta tacggaattg
      181 atgcggtgct tcgaatcgtg agtcatttgg cgtttatcta cttagccttc tggtctttac
      241 gttcgttacg cattgaaact tttttcagac caatgcataa tacgcaaatt agaatggcaa
      301 tcgtactttt ttcaattttt ttagggtaca ctgcaagtaa ttttttctta gaagttatcg
      361 ctttatgccg aaatcttttc gttagcctgt aaattttaaa aaaaggtact attttaaaag
      421 caaggatgtt ataatagaaa agattttgag taagtgtatg ctcattggaa ttttttggag
      481 ggaacaacat ggaagagatc atcgtaagag gtggcaatca attaaatgga acagtaagaa
      541 ttgaaggagc taaaaatgct gttttgccta ttttagctgc tagtttgtta gcagaagaag
      601 gtattacgac attagacaac gttccgattc tttctgatgt cttcactatg aatcaagtga
      661 ttcgtcactt aaacgtagat gtagattttg atgaacaaaa gaatcaagta acaatcgatg
      721 cttcaagaca attagaaatc gaagcgcctt atgagtatgt tagtcaaatg cgtgcttcaa
      781 tcgttgtgat gggtccgcta cttgccagaa atggacatgc aaaagtagct atgcctggtg
      841 gatgtgcgat tggtaaacga ccaatcgatt tgcatttaaa aggatttcaa gctttaggag
      901 caaaaattat tcaaaaaaac ggctatatcg aagcaattgc ggatgaattg atcggtaaca
      961 cgatttattt agatttccct agtgtaggtg ctactcaaaa tatcatgatg gctgccgtaa
     1021 aagcaaaagg aacaaccatc attgaaaacg tagctcgcga accagaaatc gttgatttag
     1081 caaacatttt gaataaaatg ggcgctcaag tctatggagc tggtactgaa acaatgcgta
     1141 ttgaaggcgt ggatcattta catgcagtca accactcaat cgtacaagat cgtatcgaag
     1201 caggtacctt tatggttgct gctgcaatga cacaaggcaa tgtgctgatt gcagatgcta
     1261 tctctgaaca taaccgccca ttgatttcta aattgatcga aatgggtgca gaaatcattg
     1321 aagaagaagg cggagtccgt gtcatcggac caaaacatat cttacctaca gatgtaaaaa
     1381 cgatgcctca tcctggtttc cctacagata tgcaagctca aatgacagct atccaattag
     1441 ttgcagaagg tacaagtgta gtgactgaaa cagtgtttga aaatcgtttc caacatttag
     1501 aagagatgcg ccgtatgaac gctcatgtca aaatcgacgg aaatgtagca atcatggatg
     1561 gaaaccatga attgcaaggt gcagaagtat atgcaacaga cttgcgggca gcagcagcat
     1621 tagttttagc tggcttgaaa gcaaatggaa tcacacgtgt gcgtaacttg aattatctag
     1681 accgtggata ttataatttc catatcaaac tacagcaatt aggtgcagac gttgaacgtg
     1741 tagacatgga tcaaacttct gctgaaaaaa cagcacaaac gattgcttaa ggaagtggct
     1801 gaatgagtaa ccaacgatat atcttattaa ctttgataaa gattttagtg gtgatcttat
     1861 tgcttatcct tctttttgtg gccggaacga tgatcggata tggtgtcatc ggtggcggca
     1921 atccatttaa agtattccag ccaagtttgt ggattcatat tcgagacttt tttcattgat
     1981 aaataagaat cacagtggtt ctagtcgata tgaagtgagc catgaagcag aacatgacaa
     2041 aagaaaacag aagggaacgt tgacgttagt cgcgatccct ttttttagta aaagaaaaga
     2101 aggaatctaa ttgatgaaga agatattgat ttatttagtt agaggatatc agcgctttat
     2161 ttcgcctgca tttccaccta gctgccgtta ttacccaacc tgttccaatt atatgataaa
     2221 agcaattcaa acccatggtg cactaaaagg gacggtaatg gggactgcac ggatcttacg
     2281 ctgtcatcct tttgtcaaag gcggtatcga ttatgtacca ttgacattcc aattaacgcg
     2341 aaataagaaa gataaaaaag aaccagatta taaaaaggtt gtgaaaaata cgagtagcac
     2401 tgagcgataa agaaaaagag gaaaattatt gtcaaagtaa caactccgca actacttata
     2461 tgggggacaa aaaatgatcg aagaactaaa caagaaagat ttaccatggt ccttgttatt
     2521 agatgcagat cctgataaag aaaaagttca agtatatgtt tccagaggga gtggctggat
     2581 atggaaagaa aaagaaaaaa caataggtgt actgatctat gtggcaaggg aaaaagaata
     2641 cgaaatcgtc aatgtagcag tagccccaac gtatcaagga aaggggattg gcggaaaatt
     2701 acttgagaca gcttttcaga agctttcaca actcgcttcc agtcaaactc agatcatcat
     2761 acgaaccggc agtactagca gtgcggcgtt acatctctat caaaaaatgg gctatgtgga
     2821 gattggtcga gtcaaagact attttatcca tcattactct gagcccattt ttgaaaatgg
     2881 agatcaatta agggatcaag taattttagc aattcactta cctaaaaata gctttaacta
     2941 ataaactttc aattcgagaa aatgctttca aatgctaaat agatacatta caatagcttt
     3001 agcaaacaaa aaggagaata tttttatggg aatattagtt tttgattttg gtggatcggc
     3061 tgtcaaatat ggttgttggg atggcaaaga gattaaaggt aagggacaat ttgcaacgcc
     3121 tgaatcttgg gaagaaatga aagcgcaatt atttcaagtt tataaaaaaa tactcgtttc
     3181 ttttgaagtc gaaggaattg gaatcagctc tcctggagta gtgaataatg agaaacaggt
     3241 gattgaggga atcagtgcga ttccttatat tcatggattt aatattttcc gagatttgga
     3301 aactctgttt caactcccag taacgattga aaatgacgcc aactgtgccg gaatggctga
     3361 attttatgaa ggtgctgccc gtgattatca agatgttgct tttgttgtgg caggaactgg
     3421 gattggcggg gcgttgttca cgaaaggaca aatcaataaa ggggcacatc tttatggtgg
     3481 agaatttggg ttgatgttct taaatggcga taagacattc agtaaacttg gtacagcggt
     3541 acagatggct tggcgttatt gtgaacgcaa aggtctcgat aaaaattctt ataccggaaa
     3601 agatgtcttt gaattagcag aaactggaga tgcaatagca aaagaagaag ttgagagttt
     3661 ttatacgtat cttacaaagg gattattcag tattcaattt tcgtttgatc cagaagtgat
     3721 cgtattaggc ggcggtgttt ctgcaaaaga agggctcatt gatgagatca aaagccgaat
     3781 gaagaagctg acagaacaat tcgatctgca tgatttcgaa ccgcagattt tattatgtga
     3841 atacagaaac gatgctaatc ttgtaggtgc agcagcaaac tatctcgctg tacgtcaagg
     3901 ggagaattaa gagctttaga gaaggtgcag cttgcataaa agaagtgaat gaaatgaata
     3961 ggagattttt acctaaggaa tgttttattt gctgcagtat gataaaatat ctgagaggtg
     4021 aaaatgatgt caaaaaaaaa taaagatgtt gaagtgcgta tagaagaagt gaaaaaagat
     4081 atcaaaggta agaattatga agtcactcag ttatttatag ggaaaaaaat gattggtgag
     4141 atcttagcct atgggccaaa agaatatgaa attttctttg gtgaagaaga ttttggaaaa
     4201 gaaaaaagtc ttgaaaatgc gatagaaaca gtgattcgac actggaatct gcacgaataa
     4261 aaaaatatca aaaaaacttt caataaaggg ttgcaaaaat ctggaaaatt aggtattata
     4321 actaagtcag gttgatacaa acctttcgga ggagtagcga agtggctaaa cgcgacggac
     4381 tgtaaatccg ttccttaggg ttcagtggtt cgaatccact ctcctccatt tcattggggt
     4441 atagccaagc ggtaaggcaa gggactttga ctccctcatg cgttggttcg aatccagcta
     4501 ccccagtata ccacaatcgt aagctctcgt tagttcgaga gctctttttt ttgctttgct
     4561 accttgttcc ttgaagattc tgctgattta agatagagtt aagttaacta caagaattgg
     4621 agggatccac atgaaatgga aagaaaaagt agacgaagca gctgaaaagt tatatgattt
     4681 ggtcaaatca gaaaaataca atattgaagt gaatattccg aaaaaaggcg gaaaagcggt
     4741 ccaggtaaaa tcaaaacgcc caacgaatca tacaaaaaaa tggatggcta aaaaccgcta
     4801 ataataacaa aagatccata agtcggcatt ttcatcgatt ttagggtctt ttgttatttt
     4861 ctaggttgaa catatctaaa aaattgtttc tgacaatagg agtaggaaga acgatgtgtg
     4921 tctaaatcct ctgttgatgg atagaaacct ttatcttttt cagttgtgaa tcaggtatat
     4981 accagttata atagaagaaa cgaaaggacg ggtgtacgtt atgtcgaatc aattacctgt
     5041 atatatacag attcatgacc aattaaaaaa tgaaatcgaa caaggtatat ggaaagtcgg
     5101 agaccgtctt ccttcagaaa gagaattagc agtgaaattc aatgtcagcc gtatgacgtt
     5161 gagacaagca attcaaaact tggcagatga aggaatctta gaaagaaaga tcggttcggg
     5221 tacatatgtt gcgcgagaaa aagtacaaga aaaaatgtct ggtgcaacaa gttttacaga
     5281 aattatggaa tcgcaaaatc gagtaccgtc tagtcggaca atctcttatt tcttgacttc
     5341 gccaagctca agtgaaatgg aaaaactaaa cttgagtaaa gatgacacga tcttgcgaat
     5401 ggaacgtatt cgtttcgcag atgatgtccc tatttgtttt gaagttgcca gtgtgccaca
     5461 aaagctgatc gatggctaca gcaaagcaga gattactgaa tcgttgtatc gcacgctaga
     5521 agaaaaaggc gggaaaaaga tcggtccagc gaatcaaacg attactgctg tgttggcttc
     5581 tgagaaaatt gctgactatt tagaggtaaa acgaggagat gcagttcttc gtttgcgcca
     5641 gatttcttat tttgaagacg gtacgccgtt tgagtatgtg cgtacgcaat acgtaggaaa
     5701 tcgttttgag ttttatttgg aaaaataaaa aacaggtgat ccctgattcc ttacttagaa
     5761 ataaaagaga atcagaatca cctgttttta tatatgatca gttaccagtt gctcgtgata
     5821 agaaacagga ccctcttcaa attggacttg tccgttcagc agctcaatga tggattcttt
     5881 aaagtggtca gcactttttt catctacagc acagatgacg ctaacttgat cagtatattg
     5941 gatttctttt ataaaaaaat gttggagttc aagataattc tgtaattttc ctagttgcgg
     6001 atacgtgatc atcacagaga tctcctgttg caaggtacct tctacgattc caatgtcaga
     6061 caatgcttga gagacagcat gagtgtatgc tcgtattaat ccgcctgtgc cgagtttcgt
     6121 tcctccaaaa tatcgagtga ctactgcagc tacattgatc agttgatttt ttttgaggac
     6181 ttccaacatc ggtacgccag ccgttccact tggttctcca tcatcgctgc ttcgctggat
     6241 ttcatttttt tctccaatga tgaaggcgct gcagttatgg ttcgctttcc aatgttcttt
     6301 ttttatttct tgaatgaaac tttttgcttg gtcctctgat tctattcgtc taagagaaca
     6361 gataaagcgt gattttttga tctcaatctc actttgtcca ttttctttta ttgtacgata
     6421 gcgctcgatc atcttgtcac ctcttttttt cattttcttt attcagtata aataaagttc
     6481 taggttgttt caacactgta acggttgcgc aatcgctgta gtatgcgata ataaagacaa
     6541 aggtaggtgg atctggggat gcaggaacga atgaaaaagt acgatgcaat tacgcattat
     6601 ttgaagaaca atggcggctc tcaagtaacg ttgaccttta cccaatttga cgaactgctg
     6661 tttcccagta acggacttcc caaaacagca agggaaagta cagattggtg ggcaaatgat
     6721 tataaacacc ctgaaaaagg tgcctatggc tggataaatg caggctatga agtagttgtc
     6781 atcaatttgg acaaagaata tgtcgtgttc aataaattag tgaaatctag ttggctattt
     6841 gattaaataa accgcgttta gaaaacgttg acaaatttct ttctttttat ataatagagt
     6901 aacagacgcg aagaaaacgt ttgcgtagtt tgttttaaaa aggagagaaa agtatggcta
     6961 aaaaaacaat catgcttgta tgttcggctg gtatgagcac aagtttacta gtaacaaaaa
     7021 tgcaaaaagc tgctgaagca aaaggaatcg actcggatat tttcgctgtg tccgcttctg
     7081 acgcagataa caatcttgca aataaagatg tagatgtact gttattagga cctcaagttc
     7141 gctttatgaa agctgatttt gaaaaacgtc tggaaccaaa aggcattcct ttagacgtca
     7201 tcaatatggc tgattatggt atgatgaatg gcgaaaaagt gttagatcaa gcaatcacgc
     7261 tcataggtga ataaatagaa cagtataact atcgaacaag cgatccaaaa aatagatcgc
     7321 ttgttttttc gtatattttt ttagggcgaa taatatgaat catttttggg gtagaaggct
     7381 tctgacgaga gatattaaag caatagactg tgaggaggta aatttaccaa attatgagaa
     7441 gatcgcagca gtggaacgtt tgggcaactg gattctttgt catcgctgtg gaatgaggac
     7501 gtccgtcatt gatggacagc ttgcagatta cggttatttt tgtacacatt gtctatattt
     7561 agggcgatgc gacactcaac aagatttata tttgtttgat cagcctgaag caaaacctcg
     7621 agaagtcgtc tttttttgga caggacagct tactgaaaaa caaacggaaa tcgcaaagag
     7681 aatactttgc cattcagaaa aaagacatca tcttatttgg gctgtaacag gtgcagggaa
     7741 aacagagatg ctttatccta ttttagtgga aactttaaaa gctggaggaa gagtagcgat
     7801 ttgtacaccg cgagtcgatg tttgtaatga attgttttta cgctatcgtt atgtttttcc
     7861 agaagaaaaa atcacgttgc tccatgggaa tacagcaacg gcttatacct ttacttcttt
     7921 tgttatctgt acgattcatc agctataccg tttttatcgg tcctttgatt taatagtgat
     7981 cgatgagatc gatgcgtttc cttataacgg ggatgcagga ttagctcatg ctgtgcaaac
     8041 agcgattaaa ccagacggac gattcatcta cttaagtgcc acacctgata aaaagttact
     8101 aaaggaaatc aagcagacgt ttgacatcca caaacttgct ttgcgctttc atcggcgttt
     8161 acttcctgaa cccgtcttgg ttttcatgaa taattggcga caaaaatgca agaacaagaa
     8221 gaagataaaa tctttggtgc ggatcattca tcaattaatt aaagaaaacc atatcctttt
     8281 attttgtcca agtatcacga tgatgcatcg tttgaaagcc acacttcaag aggctcttcc
     8341 gagatatgag atcacagatg tttcttcaag agacaatcag cgggcggaaa aagtccagaa
     8401 aatgcgagaa gcaaaatatg acatactgtt aaccacgatg atccttgaac gtggagtcac
     8461 atttgaaaga atatccgtta tcgttttagg agcggatcat ccggttttta ctaaatcttc
     8521 tttggtacaa atcgcaggaa gagccgatcg taaagggcct tatacgaaca gtcaagtata
     8581 ctttttctat gaagaaaaaa ctgcagctat tcgaaaagct tgtcaggaaa tcaaagaaat
     8641 gaatgaagaa gggaagaaga tgctgtgagg tgcagttgct gcagtaaaga tatcgtccaa
     8701 aatttaacat tcagcgagat tattggtgtt tcgaaataca gattacctca gacttgttca
     8761 gtttgtcgcg cacaatttca tttattggaa acaagaggct gttctggctg tcaaaaacca
     8821 atccatttca atctagagag tacgaaaaag aaagtaaagt ctattcccgt ttactgcagt
     8881 gactgtcaag aatggaagaa gaaatatccg acctatgatt ttcagcaccg tgcctttttt
     8941 gtgtacgatg aaaatatgaa ggcctggctc aatcaataca agtttatggg agatatccga
     9001 ctagcaggca cctttgcata taaatggacg cagataaaaa aagaataccc gacatacatc
     9061 tattgtccaa tacctttatc tgaaaaaagg cgaacagaaa gaggattcaa tcaagtaagc
     9121 gagatgttga aagcagcaag aattcctttt cagatgctac tcaaaagaga gaagcatctg
     9181 ctggctcaag caaaaaacac gcgaaaggaa agattggaga tgcctcaacc ttttgaactt
     9241 gcagtcacct tttctaagat acaaaataaa aaaatattga ttattgatga tgtctataca
     9301 acaggacaga ctcttttcca cgcagcagat tgtttgcagg tcgcacagcc gaaaatcatc
     9361 cgaaccattt ctttagcaag atgaattaga tgtggaaatt tgatagcgat ttaatttgca
     9421 aataacctag agttcgatat aatagaatta agaagaggga aagagtggtc cttctccttc
     9481 ttgattgtgg tagacgaaag gggtaatttt tatgtttaga tataatgtac gcggcgaaaa
     9541 tatcgaagta actgaagcga ttcgcgacta cgttgagaaa aaagtaggca aactagaacg
     9601 ctatttcagt gacgcacccg atgcaacggc gcatgtgaac ttaaaagttt atacagaaaa
     9661 aacagcaaaa gtggaagtaa cgatcccatt accttatctt gtattacgtg ccgaagaaac
     9721 ttctccagat ttatacgcaa gtatcgattt ggttgttgac aaattggaac gccaaatccg
     9781 caaattcaaa acaaaaatta atcgtaaatc ccgtgaaacg ggcatgaata ctgcagatgc
     9841 tactgcttta ttcaatgaag aggacgaaaa cgaaaatgat tctgaattag atatcgtacg
     9901 tacgaaacgc ctttcattga aaccaatgga tagtgaagaa gcagtcttac aaatgaacat
     9961 gctgggacat aacttcttta tttttgaaga tgcagaaaca aatggtacaa gtatcgttta
    10021 ccgccgtaaa gatggaaaat acggactgat tgaaacagac tgatcagcct tacttaaata
    10081 acaa
//
LOCUS       DARBET010000086        10049 bp    DNA     linear   BCT 07-NOV-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4027
            SAMEA114569841-rid20112183.denovo.088, whole genome shotgun
            sequence.
ACCESSION   DARBET010000086 DARBET010000000
VERSION     DARBET010000086.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA114569841
            Sequence Read Archive: ERR12203666
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 10049)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10049)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001972618.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001972618.1
            Genome Coverage       :: 132x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/07/2023 13:18:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,041
            CDSs (total)                      :: 2,975
            Genes (coding)                    :: 2,908
            CDSs (with protein)               :: 2,908
            Genes (RNA)                       :: 66
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 67
            CDSs (without protein)            :: 67
            Pseudo Genes (ambiguous residues) :: 0 of 67
            Pseudo Genes (frameshifted)       :: 26 of 67
            Pseudo Genes (incomplete)         :: 28 of 67
            Pseudo Genes (internal stop)      :: 20 of 67
            Pseudo Genes (multiple problems)  :: 6 of 67
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10049
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA114569841-rid20112183.denovo.088"
                     /isolate="VRE4027"
                     /db_xref="taxon:1352"
                     /country="Denmark"
     gene            complement(513..737)
                     /locus_tag="SCJ86_002422"
     CDS             complement(513..737)
                     /locus_tag="SCJ86_002422"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002367150.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEG1169939.1"
                     /translation="MKSDKYKNRKHTSWKTFREIIGSYLAVVVFLPLLYMAMTEQTRF
                     IGVDTQYIAETGFLFIFILIFAFYKSFHTK"
     gene            928..3705
                     /locus_tag="SCJ86_002423"
     CDS             928..3705
                     /locus_tag="SCJ86_002423"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287111.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma 54-interacting transcriptional regulator"
                     /protein_id="HEG1169940.1"
                     /translation="MNKLLKKEILCYLENQTAFFDLEHMNEVFTAQRLADHFGVKRNT
                     VSHYLNQLNDEQLLVKINSRPVIYFHKEAFEKQFFKLRTNFYFSIDDLKEEQPFFAQP
                     KDLFSFMIGYDASLKESIEQIKTALYYPDGGLPLLITGESGTGKSYLVNLVYQYCLLH
                     DLLEDSAPFITFNCAQYADNPELLTSNLFGHVKGAYTGAEENKKGAFEAADGGILFLD
                     EVHRLTPEGQEKLFTYLDQGVIYRMGEPNLSRRIKTRLFFATTEEITSHFLTTFIRRI
                     PIRIELPALNQRSRNERLELIYSFFLEEQRKMGSPLSVSGPALSLLTSQKLSGNIGEL
                     KNIVKVSAAKAYAEQREQPEIHVTIHHLQKELLAQPITKNTAQQGIYITQDQTLEQLM
                     EKQQPEQQRIVKSFEQILIDFKKNNCLLYSCEGKLKQEVIQLFDFLLFETSRQEKHEL
                     LVYWTQSIRETFRQMETAYQIKFDGNSVYALSYYLYQRSTIKWLPEEHDIIQLIKELE
                     QQVSQSYPSSYHYVQRILELSKIKLDIEDTSMDRILLTIYLKKAKWSKEKRLPKAMIA
                     AHGYATASSMANVVNRLLQEDLFESFDMPLDVTPQQIASEILDYCENSDVSNGLIILV
                     DMGSLKEIHQFFKKQLSVPLLILNNVTTPLAITVGECLQKNVSLEEIAEETVRQIQPE
                     WRLLYPEENKPKALITTCFTGIGTAVHLSELLEKSLPTACQLKIIPYEYQQLKDKKNS
                     DPLFSIYEVVGMIGTTDPDITNIDYLSLEDLISGEKMSVLAEWLDTTMNASEKEIFNQ
                     RIIRNFSLEKVLDSVTILDTEKVMGEIDHFMRELEVQLNRSISNPKKLALYVHVSCLI
                     ERLIRQMPIETYQGLEKWKQCQKEGLSAIKSAFSVIEEHYSVMIPNSEIAYIYDILSE
                     DTEFLSIEEEF"
     gene            3786..4205
                     /locus_tag="SCJ86_002424"
     CDS             3786..4205
                     /locus_tag="SCJ86_002424"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319018.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS mannose transporter subunit IIA"
                     /protein_id="HEG1169941.1"
                     /translation="MDREIILASHGKFASGILDSLELIYGKNHSITVFDCYTDENFDL
                     AETVRQLFMRYKDKEIIVLTDLFGGSVNNEFLQYINRDHVYLVAGLNLPLLIELVSRL
                     DAEVETTVLIQQVLTDSKKMIQFCNERLNQKIEEDEF"
     gene            4217..4687
                     /locus_tag="SCJ86_002425"
     CDS             4217..4687
                     /locus_tag="SCJ86_002425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629538.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HEG1169942.1"
                     /translation="MIVLTRVDHRLLHGQVAFSWTQTIGADCILIANDDVPTNEIRKT
                     TIKLAKPQGVKLVIKSIDDSIAALKSGVTDKYKLFIVVESIEDAYKLAEAYPEIKTIN
                     LGGIKAKPGTRSIGKAVNILPEEEERLKRLIDKQIEVEIRQVPADKKVDVREVL"
     gene            4704..5483
                     /locus_tag="SCJ86_002426"
     CDS             4704..5483
                     /locus_tag="SCJ86_002426"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HEG1169943.1"
                     /translation="MIVQAVLLGIVAFVAQSEYALGTSLLSRPIVTGLFTGIVLGDVK
                     TGIIMGATLELAFIGSFSVGASIPPDVVTGGILGTAFAITAGAGTETALLLGLPIATL
                     TLILKNIYLGLLIPIMNHKADNYAEEGNYKGVEHMHLLAGIGLSLMLGVVVMVSYMVG
                     SNTIGNLLNMIPDFVQKGLSVATGLIPALGFAMLARLLINKQVAPYFFLGFAIASYLE
                     IPVTGVAIFGAILAVVVVNIMNVRQLPLQTTAGEVEEDEEF"
     gene            5470..6291
                     /locus_tag="SCJ86_002427"
     CDS             5470..6291
                     /locus_tag="SCJ86_002427"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375593.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS system mannose/fructose/sorbose family
                     transporter subunit IID"
                     /protein_id="HEG1169944.1"
                     /translation="MKNSEIKNSKITKKELNHVFWRSFQMEFSWNYERQMNMAYVYAM
                     IPILKKLYQSKEEMAAALKRHLEFFNTTPHIVTLILGITAAMEEENKDDPEFDVTAID
                     SIKTSLMGPLAGLGDSFFWGTLRLIATGVGTSLAMQGNILGPILFILIFNIPHILFRY
                     LATGWGYRLGTGFLKKIQANGMMESLTLGASIIGLMVVGAMTATMITINIPLKIGSGE
                     NAVTVQSIFDDIVPNILCLGAFGIVFYLLKKEVKPLTILIGLAFFGIFGSWIGIF"
     gene            6308..7357
                     /locus_tag="SCJ86_002428"
     CDS             6308..7357
                     /locus_tag="SCJ86_002428"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287118.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SIS domain-containing protein"
                     /protein_id="HEG1169945.1"
                     /translation="MFTMQDYIYEEKEVLSTILKKNDFSTREHMKKTVNLLILATGSS
                     YNACLAAKPALESYGDLTVDIQEPFYFFNYGKLSPSIDTVIAVSQSGKSASTVEAVKM
                     IQKQGLPVVAITNDVQSPLALEAAQIIDLGAGVESVGFVTKGYSATVLQLLLLGIGIG
                     ISKNKISKKIEQDYMQQLRKIINHLPAIIQKTEEFFDGYQSLFRLAQRFVTIGYGPNW
                     GTAKEAETKLTETIRVPSQGFELEAYMHGPYLEADASHLLFFIEGESVNKERSQKLQR
                     YMSRYVGETLTITTKKARNEKTLGLAIECDEYLSVLALVVPFQLFAYKTAVAKGIDLN
                     KKIFEDFDTVLKSKL"
     gene            7370..8374
                     /locus_tag="SCJ86_002429"
     CDS             7370..8374
                     /locus_tag="SCJ86_002429"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344006.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SIS domain-containing protein"
                     /protein_id="HEG1169946.1"
                     /translation="MLKFNEEEQIKAIKGALALRPQVEEIIDKLYAEKFDAVYYLGIG
                     GTYASSMQAVTYMNGKSNLPVFVQHAAEYYTTGNKRLTKDSIVILSSVTGTTQEVVKA
                     VEQIKKVGATLIGFIDKANSKLSQLCDFVVTYPAPGTEQIKFFMAADRLMYLNGEFEA
                     YSEYYEQLERYLPTGLVEAEKKADAFGLSFAEKHRRDSMHYFIGAGNQWGAVYSYAMC
                     YWEEQSWLPSKSIHAAEFLHGTLEIVEETTPVTLFLGEDEQRVLSERVAKLLPKICSN
                     YTLIDTKDYPVEGISEKYRGRVLSFLLMHVVTQRIDAHVEQLNCHPLEIRRYYRQFDY
                     "
     gene            9007..10026
                     /locus_tag="SCJ86_002430"
     CDS             9007..10026
                     /locus_tag="SCJ86_002430"
                     /EC_number="1.1.1.94"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291040.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent glycerol-3-phosphate
                     dehydrogenase"
                     /protein_id="HEG1169947.1"
                     /translation="MKQKIAVLGPGSWGTALAQTLAENGHDVRIWGNVPEQIDEINKY
                     HTNQHFLPDLTIPESIIGYKELAEAVDNADAILFVVPTKAIRSVAQELIGKMNTKPII
                     IHASKGLEQDTHKRISEVLAEEIPEEKRQAIVVLSGPSHAEEVAVHDITTITAASIDQ
                     KAAAYVQKLFMNDYFRIYTNNDVIGVETGAALKNIIAIGAGAIHGLGFGDDAKAAIMT
                     RGLAEISRLGVAMGANPLTFIGLSGVGDLIVTCTSVHSRNWRAGNLLGQGHKLEEVLE
                     NMGMVVEGVATTKAAVELAQQLGVEMPITQTIYNVLYNGEDIKKAAKEIMLRDGKMEN
                     EFSLR"
BASE COUNT         3369 a         1655 c         2096 g         2929 t
ORIGIN      
        1 tagatgaaac tctatttact tcttttttaa tttgttcttg caatcttttt tgctacgtaa
       61 tttttgtaaa aaagtgggtt gagagaaatc taattgattt tcaaagaagg agtatgaaaa
      121 tgagaaaaat atttattcgt ttattgtggg tactatctta gaggaataaa taatagtgga
      181 gaaaactatg gtttggcgca gattctatca ggtttgatgg aacgaagaca acctcccgac
      241 atcaagtcaa aagctacttg agattttgat ctgaaaataa aaaaagaaaa caaggtagga
      301 acatcaatgt ttctaccttg ttttcgtaat caatgggaaa aaactaagag tggtgtacgt
      361 aatcaataaa aaggatggtg acaacagtac ctctttactc tctgaaagca tcataactcg
      421 tttttcctca ttatgccaat aaattgttgt tataccacat ataaggattt gtaatagcta
      481 aaaacagaca ggaaagttga aatctgtgaa tcttattttg tatggaagga tttatagaac
      541 gcaaaaatca agataaagat gaataaaaat cctgtttcgg ctatatattg cgtatctacg
      601 ccaataaaac gagtttgttc tgtcatagcc atataaagca aaggcaaaaa tacaactaca
      661 gcgagataag atccgatgat ttctcgaaaa gttttccagg aagtatgttt cctgttttta
      721 tatttatccg atttcattga atagtcatag cctccttatt ctttcatatg ttgaaattcc
      781 agtataaaaa ctggaaggaa ctgtacgttt tttagaataa aaattataag ggataagtag
      841 gctgctgtct ataataagat atatttttca attctttatt aaagatttta ttgacaaaat
      901 gaatccgttt tctatgatgg aggaggggtg aataagttgt tgaagaagga aattttatgt
      961 tacttagaga accagacagc ctttttcgat ttagaacata tgaacgaggt ctttacagca
     1021 caaaggttag ctgatcattt tggggtaaaa agaaacacag tcagtcatta tttgaaccag
     1081 ttaaatgatg agcaactact agtcaaaatc aattctcgac cagtgattta tttccacaaa
     1141 gaagcttttg aaaaacaatt ttttaagtta cgcacgaatt tttatttttc tatcgatgat
     1201 ctcaaagagg aacagccttt tttcgcgcaa ccaaaagact tattctcttt tatgatcgga
     1261 tacgacgcca gtttgaaaga aagtatcgaa caaatcaaaa cagctcttta ctatccagat
     1321 ggcggattac cacttttgat tacaggcgaa agtggcacag gtaaaagtta tttagttaat
     1381 ttggtctatc agtattgtct gcttcatgat ctgctagaag attctgcacc attcatcact
     1441 tttaattgtg cacaatatgc agataatcct gaacttttga ctagcaattt gtttggacat
     1501 gttaagggag cgtacactgg tgcagaagaa aataaaaaag gggcttttga agcagcagat
     1561 ggtgggatct tgtttttaga cgaggtacat cgcttaacac cagaaggtca ggaaaaactg
     1621 ttcacctatc ttgatcaagg tgtgatttat cgtatgggag aacctaattt gtcacgccgg
     1681 atcaaaacac gtctcttttt tgctacgact gaagagatta ctagtcattt cttaacaact
     1741 ttcattcgaa gaatacctat ccgaatcgaa ctacctgcac ttaatcagcg tagtcgtaat
     1801 gaacggctag aactgattta ctcttttttt ctcgaagaac aaagaaaaat ggggagccct
     1861 ttgtcagtca gtggaccagc tttgtcactt ttaacaagtc agaaattatc cggaaacata
     1921 ggagagctga aaaatatcgt caaagtttca gcagcaaaag catatgctga acagcgtgaa
     1981 cagcctgaaa tccatgtcac gatccatcat ttgcaaaaag aactacttgc gcagcctatt
     2041 actaaaaata cagcacagca aggaatctat attacgcaag accagacctt agagcaatta
     2101 atggagaagc aacagcccga acagcagcga atcgttaaaa gttttgagca aatcctaatt
     2161 gatttcaaga aaaataactg tctgctatat tcttgcgaag gaaaattaaa gcaagaagtg
     2221 attcaattat ttgatttttt gctgtttgag accagtagac aagaaaaaca cgagctgctg
     2281 gtttattgga cacaatctat ccgagagacc tttcgacaaa tggaaactgc ctatcaaatc
     2341 aaattcgatg gaaacagtgt ctatgccttg agttactatc tctatcagcg aagcactatc
     2401 aaatggttac cggaagaaca tgatattatc caactaatca aagaattaga acaacaagtt
     2461 tctcagtctt atccatctag ttatcactat gtccaacgaa ttctggaatt aagtaagatc
     2521 aaattagata ttgaagacac aagtatggat cggattttac tgacgatcta tctaaaaaaa
     2581 gcgaaatggt caaaagaaaa acgactgcca aaagcgatga ttgctgctca cggttatgca
     2641 actgctagca gtatggcaaa tgtagtaaat cgtttattac aagaggatct atttgaatca
     2701 tttgatatgc cactagatgt cacaccacag cagattgctt cagaaatcct tgattattgc
     2761 gaaaacagcg atgtttcaaa tggattgatc atattagtag acatgggatc tctgaaagaa
     2821 atccatcagt tttttaaaaa acagttatcc gtaccgcttt tgattttgaa taatgtgacg
     2881 acacctttag caatcacagt aggggaatgc ctgcaaaaaa atgtatcatt agaagagata
     2941 gcagaagaga ctgttcgaca gatccagcca gaatggcgac tgctctatcc agaggaaaac
     3001 aaacccaaag cactcattac tacttgcttc actggaatcg gtacggcggt ccacttaagt
     3061 gaactgctag aaaaaagctt acctacggct tgtcagttga aaatcatccc atatgaatat
     3121 cagcaattaa aagataagaa aaacagtgac ccattatttt ccatatacga agtcgtagga
     3181 atgattggca ccacagatcc tgatatcacc aatatcgact atctttcatt agaagacctc
     3241 atctcggggg aaaagatgtc tgtactagct gaatggttgg acactactat gaatgcttca
     3301 gagaaggaaa tattcaatca gcgcatcatt cgtaattttt cattggaaaa agtactggat
     3361 tccgtgacta ttctagatac agagaaggtg atgggagaaa tcgatcattt tatgagagaa
     3421 ttagaggtgc agttgaatcg aagcatcagc aatcccaaaa aattagcgtt gtatgtccat
     3481 gtgagctgtc tcattgaacg attgatccga caaatgccaa tcgaaaccta tcaaggatta
     3541 gagaaatgga agcagtgtca aaaagaaggg ttatctgcta ttaaatctgc gtttagtgtc
     3601 attgaagagc attatagtgt catgatcccc aattctgaga ttgcctatat ctatgatatt
     3661 ttgagtgaag atacagagtt tctctcgata gaagaagaat tttgaacata agcaagttgt
     3721 tgccactaat tttggcatgc aacttgcttt tttagtatgt gtaagtgaaa agaggagatg
     3781 aaaaagtgga tagagaaatt atcttagctt cccatggaaa atttgcttca gggatattag
     3841 attcattaga gctgatctat gggaaaaatc attctattac tgtattcgat tgctatactg
     3901 atgagaattt tgatttagcg gaaacagtaa ggcagctttt tatgaggtac aaagataaag
     3961 aaatcattgt tctgacggat ctttttggcg gaagtgtcaa caacgaattc ttacaatata
     4021 tcaatcggga tcatgtctat ctcgttgccg gattgaattt gccactattg attgagctag
     4081 tatctcggtt ggacgctgaa gtagagacaa ctgttttgat ccagcaggtg ctaactgatt
     4141 caaaaaaaat gatccagttt tgtaacgagc ggctcaatca gaaaatagaa gaagacgaat
     4201 tttaggagga agaaaaatga ttgtattgac aagagtcgac catcggttat tacacggaca
     4261 agtagctttt tcatggacac aaacaatagg cgcagactgt attttgattg ctaatgatga
     4321 tgtacctacg aatgagatac gaaagacaac gatcaaatta gccaagccac aaggagtcaa
     4381 actagtgatc aaatcaattg atgactcgat tgccgcgttg aaaagcgggg tgacagataa
     4441 atataagtta ttcatcgtag tggaatcgat cgaggatgca tataagctgg cagaagcgta
     4501 tccagaaatc aaaaccatca acttaggagg gatcaaggca aaacctggta ctcgcagcat
     4561 cggaaaagcg gtaaatattt taccagaaga ggaggaacgc ttgaaacgat taattgacaa
     4621 acaaatagaa gtagaaatcc gtcaagtacc agcagataaa aaagtggatg taagagaagt
     4681 tttgtaagaa aggaagaacc tcaatgattg tacaagcagt cttattagga attgttgcat
     4741 ttgtcgcaca atcagaatat gcactaggga catcgttgct ttctcgtccg attgtgacgg
     4801 ggctgtttac aggaatcgtg ttaggtgatg taaaaactgg gatcattatg ggcgcaactt
     4861 tagagttagc atttatcgga tcgttctcag taggtgcttc gattccacct gatgttgtga
     4921 ccggtgggat tttagggacg gcttttgcta ttacagctgg agccggtaca gaaaccgcgc
     4981 ttttactagg tttgccgatt gctacgttga cattgatatt gaaaaatatt tacttagggc
     5041 ttttgattcc aattatgaac cataaagcag acaattatgc agaagaagga aattataaag
     5101 gagtcgagca tatgcacttg cttgccggta tcggtctttc cttgatgctt ggtgttgtgg
     5161 taatggtttc ttatatggtg ggaagcaata cgatcggaaa tctattaaat atgattcctg
     5221 acttcgttca aaaggggttg tcagtagcta cagggttgat tccagctctt ggttttgcga
     5281 tgcttgctcg tttgctgatc aacaaacaag ttgcaccata ttttttctta ggttttgcta
     5341 tagcttctta cttagagatt cctgtgaccg gagtagctat ttttggtgcg attttagcag
     5401 ttgtggtagt caatattatg aatgtccgtc aactaccact tcaaacaact gcaggggagg
     5461 tagaagaaga tgaagaattc tgagatcaaa aacagcaaaa taacgaaaaa agagctaaat
     5521 cacgtatttt ggcgttcttt tcaaatggag ttttcttgga attatgaacg tcagatgaat
     5581 atggcttatg tttatgcaat gattccgatt ttgaaaaagt tatatcagtc gaaagaagaa
     5641 atggcagcag ctttgaaacg gcatctagag ttttttaaca cgaccccaca tatcgttaca
     5701 ctgattttag gaatcacggc tgcaatggag gaagaaaata aggatgatcc tgaatttgat
     5761 gtgactgcga tcgatagtat caaaacatct ttgatgggac ctcttgcagg gttaggagat
     5821 tctttctttt ggggaacttt gcgattgatt gccacaggtg tgggtacctc tttagccatg
     5881 caaggaaata ttttaggccc tatcttgttc attttgattt tcaatattcc gcacattctg
     5941 tttcgctatc tagcaacagg ttggggatat cgcttaggaa cgggtttttt gaaaaagatc
     6001 caggcaaacg gtatgatgga aagtttgaca ttaggcgcgt ctattattgg actgatggtc
     6061 gttggagcaa tgactgccac gatgatcacc atcaatattc ctttaaaaat cggttcagga
     6121 gaaaatgctg tgactgttca aagtattttt gatgatatcg ttccaaatat cttatgtctt
     6181 ggagcatttg gcatagtgtt ttatctattg aagaaagaag tgaaaccatt aacgatcttg
     6241 atcggactag ctttctttgg aatttttggt tcgtggatag ggatttttta aaaaaggagc
     6301 agagaaaatg tttacgatgc aagattatat ctatgaagaa aaggaagtac taagtacgat
     6361 cttgaaaaag aatgattttt cgacaagaga acatatgaaa aaaacagtga atttactgat
     6421 cttagcaacg ggttcatctt ataacgcttg tttagcagcg aagccagcat tggaatctta
     6481 tggtgatctt actgtcgata ttcaagaacc tttttatttt ttcaattatg gaaaactttc
     6541 tccttcaatc gatactgtga ttgctgtgtc acaaagcggg aaaagtgctt ctactgttga
     6601 agcagtaaaa atgatacaaa aacaagggtt acctgttgtt gctatcacaa atgacgtgca
     6661 aagtccactt gctttagaag ctgcacaaat tattgattta ggtgccgggg tagaatcagt
     6721 aggcttcgtt acaaaaggct attctgcgac tgttttacag ttactactct tagggatagg
     6781 aattggaata agtaaaaata aaatcagcaa aaaaatagaa caagactaca tgcaacagtt
     6841 gcgcaaaata atcaaccatt taccagcgat tattcaaaaa acggaagaat tttttgatgg
     6901 atatcaaagt ttatttcgtt tagcacagcg tttcgtcacc attggatatg gtccaaactg
     6961 gggaacagct aaagaagcag agacgaaatt gactgaaaca atccgcgttc cgtcacaagg
     7021 atttgagtta gaagcttata tgcacggccc atacctggag gcagatgctt cgcatttgct
     7081 attctttatt gaaggggaaa gtgtaaataa ggaacgttcg caaaagttgc aaagatatat
     7141 gagtcggtac gttggagaga ctctcaccat taccacaaaa aaagcaagaa acgagaaaac
     7201 acttggatta gcaatagaat gtgatgaata tctgtctgta ttagcgcttg tcgttccgtt
     7261 tcaactattc gcttataaaa ccgcagtagc taaaggtatt gacttgaaca aaaagatttt
     7321 tgaagacttt gatacagtct tgaaaagtaa attataagag gtggaaatca tgctgaaatt
     7381 caatgaagaa gaacaaatca aagcaataaa aggcgcacta gctttacgtc cgcaagtaga
     7441 agaaatcatc gataaacttt atgcggaaaa atttgacgct gtctattatc taggaatcgg
     7501 tggaacgtat gcttcctcga tgcaagctgt cacttatatg aatgggaaaa gcaacttgcc
     7561 tgtctttgta cagcatgctg cagaatatta cactactgga aacaaaagat taacaaaaga
     7621 ttctatcgtt attttgtcct ctgttaccgg tacaactcaa gaagtagtga aagcagtaga
     7681 acaaatcaag aaagtaggag cgacactgat tggatttatc gataaagcga acagtaagtt
     7741 gtctcagcta tgcgattttg tggtcactta tccagctcct ggaacagaac aaatcaaatt
     7801 ttttatggca gcagatcgat tgatgtattt gaatggagaa ttcgaagctt attcagaata
     7861 ctatgaacag ctcgaacgtt atttaccaac agggttagtg gaagcagaga aaaaagcaga
     7921 tgcatttggc ttatcatttg cagaaaaaca tcgacgtgat agtatgcatt attttattgg
     7981 agcagggaat caatggggag ctgtttattc atatgccatg tgttactggg aagaacaaag
     8041 ctggcttcca tcgaaatcta tccatgctgc cgagtttctt cacgggacgt tagagatcgt
     8101 agaagaaaca accccagtca cattattttt aggcgaagat gaacagagag tgttatctga
     8161 acgtgtggct aaacttttgc caaaaatttg cagcaattat acattgatag acacaaaaga
     8221 ctatccagta gaaggtatca gtgaaaaata tcgcggacgt gttttgtctt tcttattgat
     8281 gcatgtagtg acacaacgca tcgatgctca tgtggagcag ttgaactgcc atccattaga
     8341 gatacgccgc tactatcgtc agtttgatta ttaggataga ctggataaaa agaatataaa
     8401 aaataaagag agcagcgata actgctccct ttattttttt aaaatattgg catcaattac
     8461 gttataaaac acattttttg cattatctac agttcaactt ataatctgtt taatgggaca
     8521 tgtgggtgcc aaccattttt tgttatgcaa tagtactatt tagttgttgc atggatccgt
     8581 agtaaaagtc caagtacaac gagttgcgcc agtttacata gcgttttgta tccagaatgt
     8641 tgactaaaac aaaagttcct atgtcaaccg tcaacagaga caatctgtcc gttgcaggac
     8701 ctgacagatt tagaaatgtt caatctgtct cttatgtatt ttaaatcaat actcactagg
     8761 agaagtttcc taacctccta atagtaatgc tggtagtaac attgtcaaaa ctgcttcctc
     8821 ttaggaacaa gcaaagcata acgagtgtaa aaataaaatg tagactattg taacatatca
     8881 ttgattattt caagtaaaaa cgataaaaga agtaaacaaa cacttaaaaa caatggaaat
     8941 tagtcccaac tttaagaaat gtatactata atggaaatta tcaattattt atttggagga
     9001 atatccatga aacaaaaaat cgcagttctt ggtcctggat cttggggcac tgctttagca
     9061 caaacattgg cagaaaacgg gcatgatgtc cgtatttggg gaaatgtacc tgaacagatt
     9121 gatgaaatca ataagtatca tacaaatcag cattttttac ctgatttgac gatcccagaa
     9181 tcgattatcg gatataaaga attggcagaa gcagtggata acgcagatgc tattttattt
     9241 gttgttccaa caaaagcgat tcgttcagtc gcacaggaac ttattggtaa aatgaatact
     9301 aagccaatca ttatacatgc aagtaaagga ctagaacaag atacgcataa acgaatctca
     9361 gaagttttgg ctgaagaaat tccagaagaa aaaagacagg caattgttgt attatctggt
     9421 cctagccatg ctgaggaagt ggcagtacat gatatcacaa caattacagc tgcaagtatt
     9481 gatcaaaaag cagcagccta tgtacaaaaa ctgttcatga atgattattt caggatttat
     9541 acaaataatg atgtgattgg cgtagaaact ggtgcagcac tgaaaaatat cattgcaatt
     9601 ggtgccggcg ctatccatgg attaggattc ggagatgatg caaaagcagc aatcatgact
     9661 cgaggattgg cagaaatcag ccgtcttggt gtcgcgatgg gtgccaatcc tctgacattt
     9721 atcggtttga gtggtgtggg ggacttgatc gttacttgta caagtgttca ttctcgcaat
     9781 tggcgtgccg gtaacttgtt aggacaggga cataagctgg aagaagttct agaaaacatg
     9841 ggaatggtcg ttgagggtgt ggcaacgaca aaagcagcag tagagctagc tcaacagcta
     9901 ggtgttgaga tgccaattac tcaaacaatc tataatgttt tgtataatgg agaagatatc
     9961 aaaaaagcag caaaagaaat catgttacgt gacggaaaaa tggagaatga attttctctt
    10021 cgttaaatag atgatcaata gttaatcgt
//
LOCUS       DARBET010000087         9820 bp    DNA     linear   BCT 07-NOV-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4027
            SAMEA114569841-rid20112183.denovo.089, whole genome shotgun
            sequence.
ACCESSION   DARBET010000087 DARBET010000000
VERSION     DARBET010000087.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA114569841
            Sequence Read Archive: ERR12203666
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 9820)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9820)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001972618.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001972618.1
            Genome Coverage       :: 132x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/07/2023 13:18:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,041
            CDSs (total)                      :: 2,975
            Genes (coding)                    :: 2,908
            CDSs (with protein)               :: 2,908
            Genes (RNA)                       :: 66
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 67
            CDSs (without protein)            :: 67
            Pseudo Genes (ambiguous residues) :: 0 of 67
            Pseudo Genes (frameshifted)       :: 26 of 67
            Pseudo Genes (incomplete)         :: 28 of 67
            Pseudo Genes (internal stop)      :: 20 of 67
            Pseudo Genes (multiple problems)  :: 6 of 67
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9820
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA114569841-rid20112183.denovo.089"
                     /isolate="VRE4027"
                     /db_xref="taxon:1352"
                     /country="Denmark"
     gene            complement(<1..195)
                     /locus_tag="SCJ86_002431"
     CDS             complement(<1..195)
                     /locus_tag="SCJ86_002431"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327009.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEG1169948.1"
                     /translation="MLKISYKPSTDSKEMKKEYETVNDFLQGQYLEVPPLQDHFVVTT
                     VTLDGKEIEMPDQTISGLFNY"
     gene            complement(211..825)
                     /locus_tag="SCJ86_002432"
     CDS             complement(211..825)
                     /locus_tag="SCJ86_002432"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297470.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flavin reductase family protein"
                     /protein_id="HEG1169949.1"
                     /translation="MFERSPSELTERENYKLLIGSVIPRPVAVVTTKSAKDIVNIAPF
                     SYFNIVSSNRPILSLAIQRKQDAVKDTAKNILETREAVIHILDEDNVEEVNKTAANLP
                     NEESELYLTDLTLTDSVIISVPALTEAKVRFETSLYEHVEIRNKNKVTADLFLLEVQK
                     YHLNETVYDDKTGRIDPRKLNAVSRLAGNDYAAIGEIFTIARPE"
     gene            complement(972..1655)
                     /locus_tag="SCJ86_002433"
     CDS             complement(972..1655)
                     /locus_tag="SCJ86_002433"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmannosamine-6-phosphate 2-epimerase"
                     /protein_id="HEG1169950.1"
                     /translation="MDTLEQIKGGVVVSCQALENEPLHSSFIMGRMALAAKEGGAVGI
                     RANTTEDINEIKRIVDLPVIGIIKRDYDGSEVFITATEKEVEELLETQAEIIALDATS
                     RKRPTEKTTAELVQMIHEHGRLAMADISTFEEALQAQEDGFDLLSTTLAGYTEYSRKT
                     QTPDFDLLNQIIEQVDIPVIMEGHTDSPEQVEKAYELGAFSVVVGSIITRPQLITKRY
                     VEAASKANN"
     gene            complement(1764..2222)
                     /locus_tag="SCJ86_002434"
     CDS             complement(1764..2222)
                     /locus_tag="SCJ86_002434"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ASCH domain-containing protein"
                     /protein_id="HEG1169951.1"
                     /translation="MYQQVEEYVKKFQEKYPSYQSEKLGFFSFGDTEKMADELSELVV
                     KKIKTGTSSGYDLYKQENEPLPKVGQLDVVLNGKNEPVCIIKNINVTILPFSEVTKEH
                     AWKEGEGDRTLAYWRKVHAEFFKHAYKEALNIEFTEEKLVVYEEFEVIFF"
     gene            complement(2306..4069)
                     /locus_tag="SCJ86_002435"
     CDS             complement(2306..4069)
                     /locus_tag="SCJ86_002435"
                     /EC_number="2.2.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1-deoxy-D-xylulose-5-phosphate synthase"
                     /protein_id="HEG1169952.1"
                     /translation="MSTVLHVLDAINSPADVKKLSVEEMKQLAEEMRQKILQKDSDVG
                     GHVGPNLGVVELTIAFHYVFDSPKDKIVWDVSHQSYGHKLLTGRKNGFEKGHYREVSG
                     YSNQHESKHDYFTVGHTSTSISLATGLAKARDLKKESGNVVAFIGDGSLSGGLAFEGL
                     NNGGVLQSNLIVLVNDNEMSIDENHGSLYKNLAELRQTNGKSSNNLFKAMGFDYRYVD
                     NGNDLETVIRLFEEVKDIDHPIVLHVHTEKGRGYQPAIDNKMKYHWHVPFDLSTGEAK
                     QVTSGETFNMIIMDYLDKKMQTSTAPIAAVNAAIPGAFGLKTFQEKHPDHYFDVGIAE
                     QHSITFASALASQGVRPVVFQSSTFLQRAYDQLSHDLAINENPVIILVGGGTISASDR
                     THQGTFDIPYLSSIPNIKYLAPATKEDMISMLDWAFEQTAQPVAIRIPTHGVEHGTAI
                     ANDYSIPAYQTVNNGGKVAILALGGFLELGKETVKAFRKKGIEATLINPGTITENDVE
                     TLDKLKETHELVITLEDGSLSGGFGENISRYYGDSSMNVLNFGAEKEFTDSVPVGQLY
                     ERYHLTPKQIVEDSLRVLNIQ"
     gene            complement(4290..5750)
                     /locus_tag="SCJ86_002436"
     CDS             complement(4290..5750)
                     /locus_tag="SCJ86_002436"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002316287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEG1169953.1"
                     /translation="MLENYIERNIFRKVYLCEQLFEFQEIDIEQTAISLRVTTPTILH
                     DLESLAECLEYCIKEQVREKHKYKLVFKHGIALSELTQFLYGQSYFLKFLSYYFNGIF
                     TSTELADLEFISLSKVYTIKKIVLDFFKENDYLKNKEIIIPEFDSRNILLALVRYVNW
                     EGYENKNPYVEEACNELIDYVETHFFKRRYSEDEKSFIIRGIEIAIGRKAFPLGLKEE
                     DKKSAETKPLFQLVCQGLEEQKGILDLQEEDVYYIFSLFNSRNYTNENMELLRKDFEV
                     VYKNFVLNDLSLNELILQIISIAEIDDAENFIFKQSFLQFVRTLWADGQVFLPERIYL
                     LSEKEQLLYGKIVEILEEWKKKNQIQLRWNENFIRKFVKSLSLINSEQAGRQEIEIFV
                     VSESSVKQFFYRTQFHLYAEEGIAEINPILFRSLEELPDECLCAKKRVVLCDAASYQN
                     GLETEKTKIYPISLKGANIHLYHIAQELHLLKKHIS"
     gene            complement(5958..6884)
                     /gene="glsA"
                     /locus_tag="SCJ86_002437"
     CDS             complement(5958..6884)
                     /gene="glsA"
                     /locus_tag="SCJ86_002437"
                     /EC_number="3.5.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337014.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutaminase A"
                     /protein_id="HEG1169954.1"
                     /translation="MEVKELEQLIGKNFHYYQTGQVADYIPALAEVDPKQLGMAIYDL
                     DTKQLIEGGDADVRFAIESMSKVPVLLLAIKDNGIEKVFQTINTEPTGFAFNSIMNME
                     INHRKHPMNPFVNAGAIATTSLIAGKDADEKFERILSFMKEICDDPNITLNEEIYHSE
                     SRTGDINRSLAYYMKGNQMIEGDVPEILDAYFRQCSLNVTATGIAKLAAVLANKGIAP
                     WNGKQIITEESATIVKSIMTTAGLYDESGEFSVHVGVPAKSGVGGGLMAAVPNRYGIG
                     VFSPALDPFGNSAAGIQLLKDVVKELDADIFE"
     gene            complement(6907..8334)
                     /locus_tag="SCJ86_002438"
     CDS             complement(6907..8334)
                     /locus_tag="SCJ86_002438"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340024.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HEG1169955.1"
                     /translation="MQQSKTKIPAKKLTFFGFLAMTISMVVSLYEYPTFATSGFSLVF
                     FLLLGGLLWFIPVALCAAEMATVKGWEKGGVYTWVSRTLGKRFGFAAIFFQWFEITVG
                     YLTMLYFLTGALSYATGIGAIQNNKYLKLAILLIIFWAILLSQLKGTKYTSLIARIGF
                     VAGILLPALVLFGLGIHYVASGAPLQTILSMKTLIPDFSKLPTLVVFVSFILAYMGVE
                     TSASHANEMENPKKDYPLAMFVLVILAIILDTFGGLTVATTVPQHGLSLNTGVIQSLE
                     FLLRHIDPNLAWIAKILAILVCLGVIGEIASWVTSPSKALHVAAEEGLLPEYFAKENT
                     HGVPAHLMIANGIVATIWAAVFTLSGGGNNLSFLAAMSLTVVIYLMLYFMFFAGYLKL
                     VFKDDSLERAYQVPGGKVVKVIVACLGLLTSLFAFCISFVPPASIAAGQHGIYETILI
                     VSYLIVLVIPFTIYACRHKFQHAEK"
     gene            complement(8365..9579)
                     /locus_tag="SCJ86_002439"
     CDS             complement(8365..9579)
                     /locus_tag="SCJ86_002439"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629388.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ammonium transporter"
                     /protein_id="HEG1169956.1"
                     /translation="MNTLPINTGDTAFMILCTAMVCLMTPGLAFFYGGLARKNNILTI
                     MSQSFLSVGITTIMWVFGGFGLAFGRDIGGVIGNPADFFLMRHVTSFPNMFHGATIPF
                     LMFFLYQLMFIVITVPLMTGAFAGRLNLKGYIILVIFWNLLIYFPVCHWIWGGGFLDQ
                     MGFRDFAGGAVIHTTAGFGSLACILTLGQRKIPLGERSQHSSLMAAAIGTGLLWFGWF
                     GFNSGGALRADEQAVNAFGSTFIALAFAMITWLIIAKVKGNGFDFVDVLTGSVAGLAA
                     ITPCAGYVEAKSAMLIGIIAGIVCHAAVDFRKKKQWDDALDVWGVHGMGGFTGTILIG
                     IFASNSHLLTNPSSWYFLGIQVIGVIITAVYAYVLTTVILKSAMHFTTITTTKEEQEE
                     GLDFFFFGNKLN"
BASE COUNT         2913 a         2041 c         1627 g         3239 t
ORIGIN      
        1 gtaattaaac aatccgctaa tcgtttgatc tggcatttct atttccttac catctaaagt
       61 aacagtagta acgacaaaat ggtcttgcaa tggcggaact tctaagtact gcccttgcag
      121 aaaatcattc actgtttcat attctttttt catttctttt gaatctgtcg atggtttata
      181 gcttattttt aacataacta gtgctccttt ttattcggga cgtgcaatcg tgaaaatttc
      241 tcctattgct gcataatcat ttcctgccaa acggcttaca gcattcaatt ttcttggatc
      301 gatccttcct gtcttatcat catagactgt ttcatttaaa tgatatttct gtacttctag
      361 caaaaataaa tcagctgtta ctttattttt attccgtatc tcgacatgtt catataagga
      421 tgtttcaaaa cgaacttttg cttctgtcaa tgctggtact gaaatgatta ctgaatcagt
      481 taaagtcaag tcagtcaggt acagttcgct ttcctcatta ggcagattgg ctgctgtttt
      541 atttacttct tctacgttgt cctcatcaag gatatggata acagcttctc ttgtctcaag
      601 aatattcttt gctgtgtctt ttactgcatc ttgttttctc tggatcgcca aggataagat
      661 gggtcgattc gaactgacga tattgaagta actgaagggt gcgatattca ctatatcttt
      721 tgctgattta gtcgttacta ctgctactgg tctagggatg actgaaccaa tcagcaactt
      781 gtaattttca cgttctgtca attctgaagg actccgttca aacatggttt cgccttcttt
      841 ctataaaatt cgttaaaaag cagatcttga agacaaaatg aaaagtaatg cttttttcat
      901 cttcttagaa tctgctttaa actatctatc aatttttctt ttttgtcaag aatctgtgcc
      961 gtgcgtgttt tttagttatt tgctttactt gcggcttcga catatctttt agtgatcaat
     1021 tgcggacggg tgataataga tccaactacg actgaaaaag cacctaattc atatgctttc
     1081 tctacttgtt caggagagtc agtatgtcct tccataatta ctgggatatc gacttgttct
     1141 atgatttgat tcaataaatc aaaatcaggt gtctgcgtct ttcgtgaata ttctgtatag
     1201 cctgctaaag tcgtagacaa aagatcaaaa ccatcttctt gcgcttgaag cgcttcctca
     1261 aaagtcgaaa tatctgccat agctaaccga ccgtgttcgt gaatcatttg gacaagttca
     1321 gcagttgttt tttctgtcgg acgtttacga gaagttgcat ccagtgcaat gatttctgct
     1381 tgcgtctcca aaagttcttc cacttctttt tctgtcgcag taataaacac ttctgaccca
     1441 tcgtaatctc ttttgataat ccctattacc ggtaaatcaa cgattcgctt gatttcattg
     1501 atatcttctg tcgtatttgc tcgaatacca actgctccgc cttcttttgc agccaaagcc
     1561 attctcccca taataaaaga actgtgtagc ggctcgtttt ccaaagcttg acaagaaacg
     1621 acgactccgc ctttgatttg ttccagtgta tccacaatat ctcctccagt aatgttatat
     1681 aaaaatacag cttcatataa aaatgtaacc cttttctatt caggagacaa taatgagcta
     1741 tttctttcag cagacctttc ttactaaaag aaaattactt caaattcttc gtatacgacc
     1801 agcttctctt ctgtaaattc tatgttcaac gcttctttat aagcatgttt aaaaaactca
     1861 gcatggactt tccgccaata agctaatgtc cgatcccctt ctccttcttt ccaagcatgc
     1921 tctttcgtga cttcactaaa aggcaagatg gtcacattta tatttttgat gatacaaact
     1981 ggttcatttt ttccattcag gaccacgtct aattgaccaa cttttggtaa aggctcattc
     2041 tcttgtttat acagatcgta accacttgat gtaccagtct ttatcttttt tacaactagt
     2101 tcgctcaact cgtctgccat tttttctgta tccccaaatg aaaagaaacc taatttttct
     2161 gattgataac taggatattt ttcttggaac tttttgacat attcttctac ttgctgatac
     2221 ataacatcca cccctttgct ttttataatg aaaaaaccat ccagatacta gtataaacaa
     2281 ttatctggat ggtgagcata atgaattatt gaatattcaa gacacgcaga ctatcttcaa
     2341 cgatttgttt tggtgttaaa tgataacgtt cataaagctg ccctacagga acgctatccg
     2401 tgaattcttt ctctgcacca aaatttaata cgttcattga agaatcacca taataacgac
     2461 tgatattttc gccaaatcct cctgacaaac taccatcttc taatgtgatg actaattcgt
     2521 gtgtttcttt taatttatct aatgtttcta cgtcattttc tgtgatcgtt cctggattga
     2581 tcaacgttgc ttcgattcct ttttttctaa aagcttttac tgtttctttt cctaattcaa
     2641 gaaaaccgcc taaagctaaa attgctactt ttcctccatt atttactgtt tgataggctg
     2701 gaatactata atcatttgcg atagctgtac catgttctac accatgtgta ggaattcgga
     2761 ttgctacagg ctgcgctgtt tgttcgaacg cccaatctag catagagatc atatcctctt
     2821 ttgttgctgg agcaagatat ttgatgttcg ggatacttga taagtaagga atgtcaaatg
     2881 ttccttggtg tgttcggtca ctagctgaga tcgttccgcc accgactaaa ataatcactg
     2941 gattctcgtt gatcgctaaa tcatgtgata attgatcata tgcccgttgc aagaaagtac
     3001 ttgattggaa gacgactgga cgtactccct gagaagccaa agcagaagcg aaagtgatag
     3061 agtgctgttc agcgatcccc acgtcaaaat aatgatctgg atgtttttct tggaatgttt
     3121 tcagtccaaa ggcgcctggt atcgctgcat tgactgcggc aataggagct gtacttgttt
     3181 gcattttctt atccaaataa tccatgatga tcatattaaa tgtttctcca gaagttactt
     3241 gctttgcctc cccggtggac agatcaaatg gtacatgcca atgatatttc attttattat
     3301 cgattgccgg ctgataaccg cgaccttttt ccgtatggac gtgaagtaca atcggatgat
     3361 caatatcttt tacttcttca aataaacgga tcaccgtttc cagatcgttt ccattatcca
     3421 cgtaacgata atcaaatccc attgctttaa ataggttatt gctgctttta ccgttggttt
     3481 gacgaagctc agctaagttt ttatataaac tgccatgatt ctcatcaata gacatctcat
     3541 tatcgtttac taatacgatt agattggatt gtaaaacacc accattgttc aatccttcaa
     3601 aagctaagcc accacttagg gagccgtcac cgataaatgc taccacattt cctgattctt
     3661 tcttcagatc tctcgcttta gctaaacctg ttgctaagct gatagatgta gatgtgtgcc
     3721 ccactgtaaa atagtcatgt ttactttcat gttggttgga atatccgctc acttcacggt
     3781 aatgtccttt ttcaaaacca ttttttcgac cagttaaaag cttatgacca taagactgat
     3841 gggaaacatc ccaaacgatt ttatcctttg gtgaatcaaa tacataatga aacgcaatcg
     3901 ttaattctac tacacctaga ttcggaccaa cgtgcccacc tacatcgctg tccttttgca
     3961 agattttttg gcgcatctct tctgccaact gtttcatttc ctcaacagat aattttttga
     4021 catctgcggg gctattgatt gcatccaata catgtaatac tgtactcata atacattctc
     4081 tcctccgtac tatcttcttt taatgaaaat agatttgtta cactttatct attctactac
     4141 ttagagttga ctctaagtca atagcatttt aaaaaaattt cagagaaagc ttgttatata
     4201 agtagatctt cgcacgaatc aggactcaaa taaaaacctt ctacagacaa ttaaaaagct
     4261 ttgtagaagg tctcctaatt aaggtctcat caagaaatat gtttttttaa taggtgaagt
     4321 tcctgagcaa tatgatacaa atggatgttt gcacctttta atgagattgg atagatcttt
     4381 gtcttttcag tttctaatcc attttgatag ctagcagcat cgcacaaaac cactcttttt
     4441 ttcgcacata gacattcatc aggcaattct tctaatgaac gaaataaaat aggatttatt
     4501 tcagcgatgc cttcttccgc atacaaatga aattgggttc gataaaaaaa ttgtttgact
     4561 gaactttctg aaacaacaaa aatttctatt tcctgtctcc ccgcttgttc actgttgatc
     4621 aaagaaagac ttttgacgaa ttttcgaatg aaattttcat tccatcttag ttggatttga
     4681 tttttctttt tccactcttc caatatttct acaatttttc cgtacagaag ttgttctttt
     4741 tctgatagaa gatagatcct ttccggtaaa aatacttgtc catctgccca caacgtacgg
     4801 acaaattgta aaaaggattg cttgaaaata aaattttctg catcgtcgat ttcagcgata
     4861 ctaataatct gtaaaatcaa ttcattcagt gataggtcat tgagaacaaa atttttataa
     4921 acaacttcga aatcttttct taacagctcc atgttctcat tggtataatt tctactgttg
     4981 aacaaagaaa aaatataata gacatcttct tcttgtaagt ctagaattcc tttttgctct
     5041 tccaatcctt gacagactaa ttgaaataac ggcttcgttt ctgcgctttt cttatcttct
     5101 tctttcagtc caaggggaaa tgcctttctt ccgattgcga tctctattcc tcgtatgata
     5161 aaggactttt catcttccga atatcttctt ttaaaaaaat gagtctctac ataatcaatc
     5221 aactcgttac aagcttcttc tacatacgga tttttatttt catagccttc ccaattgaca
     5281 taacgtacta aagctagcaa gatgttccgc gagtcaaatt ctgggataat gatttcttta
     5341 tttttcaaat aatcattttc tttaaaaaaa tccaatacga tttttttgat tgtatatact
     5401 ttgctcaaag aaataaattc aagatcagcc agttctgtac tcgtgaatat accattaaaa
     5461 taataactta aaaactttaa aaaataagat tgaccgtaca gaaactgtgt gagctcagaa
     5521 agagcaatac catgtttgaa tacgagttta tacttatgct tctctcttac ttgttctttt
     5581 atacaatatt ctagacactc ggctagactt tctagatcgt gaaggatcgt tggtgtagtc
     5641 acccttagag aaatagctgt ttgttcgatg tctatttctt ggaattcaaa taattgttca
     5701 cacagataca cttttctaaa tatatttcgc tcgatgtaat tttccagcat tcctgataca
     5761 cgcctccagt ttttcttatg attttattta atcatatctg taaatcgtat ttttgtttcc
     5821 tttttattta gaaaagccct ttcaaaaacc aataaaaaaa gaaactggga caaaagtcaa
     5881 acaagctcac aatttcagcc tcattgtagg tagctttgag ccgtttggac ttttacccca
     5941 gcctctttca agaatggtta ttcaaagatg tcagcgtcta gttctttgac tacatctttc
     6001 aacaattgga taccagctgc gctgttacca aatggatcta gtgccgggct gaagacacct
     6061 atgccgtaac gatttggcac agctgccatc aacccgccgc ctacaccgct ttttgcaggt
     6121 acaccaacat ggacggaaaa ctctcctgat tcatcataca aaccagcagt tgtcataatt
     6181 gatttgacga ttgtagcact ttcttcagta atgatttgtt tgccattcca aggagcaatt
     6241 cctttatttg ccagcacagc agctaatttt gctatgcctg tagcggtgac attgagggag
     6301 cattgtctga aataagcatc gagtatctcc ggtacgtctc cttcgatcat ttgattacct
     6361 ttcatatagt aagcaagaga gcgattgata tcacctgttc ttgattcaga atgatagatt
     6421 tcttcattca atgtgatatt aggatcatca cagatctctt tcataaatga caagattcgc
     6481 tcaaattttt catctgcatc tttaccggca atcaacgagg ttgttgcaat cgctcctgca
     6541 ttaacaaaag gattcattgg atgtttgcga tgattgattt ccatattcat gatagaattg
     6601 aaggcaaaac cggttggttc cgtgttgatt gtttgaaaga ctttttctat tccgttgtct
     6661 ttgattgcta ataacaatac gggaactttt gacatgcttt cgatggcaaa gcgcacatcg
     6721 gcgtctcctc cctcaatcag ttgttttgta tccaaatcat aaatcgccat acctaattgt
     6781 ttgggatcca cttcagctaa tgctggaatg taatccgcca cttgtcctgt ttgataatag
     6841 tggaaatttt ttccaatcaa ttgttctaat tcttttactt ccatttgaat aaccagcttt
     6901 cattttttat ttttctgcat gctggaattt atgacggcat gcgtagatcg taaatggaat
     6961 aactaagacg ataagataag aaacaatcag aatcgtttca taaatcccat gctgtccagc
     7021 tgcgatacta gcaggtggaa caaatgagat acaaaaagca aaaagtgaag tcaaaagacc
     7081 taaacaagca acgattactt ttacaacttt tccgcctgga acctgatagg ctctttcaag
     7141 actgtcatct ttaaaaacca gcttcaaata gcccgcgaaa aacataaagt acaacatcag
     7201 gtagattaca acagtcaatg acatcgctgc aagaaatgaa agattattcc ccccgccgct
     7261 taatgtaaag acggctgccc agattgttgc aacgatccca tttgcgatca tcaaatgagc
     7321 aggaacaccg tgtgtgtttt cttttgcaaa atattcagga agtaatcctt cttctgctgc
     7381 tacgtgaagt gcttttgaag gagaggttac ccaagaagca atttctccga tgacacctaa
     7441 acaaacaaga atcgcaagaa ttttcgcaat ccatgcaaga tttggatcaa tgtgtcttaa
     7501 taggaattct aacgattgaa tcacccctgt attaagagac aaaccatgtt gaggtactgt
     7561 agtagcgacg gtcaatccgc caaaagtatc taaaatgatt gccagaataa ccaatacaaa
     7621 cattgctaat ggataatcct ttttagggtt ttccatttca tttgcatgac tggcagatgt
     7681 ttctacgccc atatatgcta aaataaagga aacaaaaaca accagcgtag gcaatttcga
     7741 aaaatcagga atcaatgttt tcatagacag tatcgtttgt agtggagcgc ctgaagcgac
     7801 ataatggatc cctaagccaa acaagactaa tgctggcaac aagataccag ctacaaaacc
     7861 gatccgagca atcaatgaag tgtatttggt tccttttaac tgtgagagta aaatagccca
     7921 gaaaataatc aataggattg ccaatttcag atatttattg ttttgtatag caccaatacc
     7981 cgttgcatag gataatgcac cagttaagaa atacagcatg gtcaagtagc caactgttat
     8041 ctcaaaccat tggaaaaaga ttgcggcaaa accgaatcgt tttcccaatg tgcgactgac
     8101 ccaagtatag acaccgcctt tttcccatcc tttcaccgtt gccatctctg ctgcgcacaa
     8161 tgccacaggg ataaaccata gcaaaccgcc taataacaag aaaaatacta agctgaagcc
     8221 tgaggtcgcg aatgtgggat attcatataa tgaaacgacc attgagatgg tcatagccaa
     8281 aaagccaaag aacgttaatt tttttgcagg aattttcgtt ttgctttgtt gcatgaatct
     8341 tcctcttttc tatttgaatt tgtttcaatt gagtttgttg ccaaagaaga aaaagtccaa
     8401 tccttcttct tgttcttctt ttgtagtagt gatcgttgta aagtgcatcg ccgattttaa
     8461 aatcactgtt gtcagcacat aggcatacac ggcagtaatg atgacaccaa tcacttgaat
     8521 ccctaagaag taccagctgc ttggattcgt tagtaaatgg ctgttagatg caaagatccc
     8581 tatcagaatc gtacctgtga atccgcccat gccatgaaca ccccacacat caagtgcatc
     8641 atcccattgt ttctttttac gaaaatcaac agcagcatga cagacgatac ccgcaatgat
     8701 cccaatcagc atggcacttt ttgcttctac ataaccggca caaggcgtaa tggctgccaa
     8761 tccagcaacg gatcctgtta gtacgtcgac aaaatcaaag ccatttcctt ttacttttgc
     8821 aatgatcagc caagtaatca tcgcaaatgc aagagcgatg aatgttgaac caaatgcatt
     8881 gactgcttgt tcatctgctc gaagtgcacc accggaatta aaaccgaacc agccaaacca
     8941 aagcagtcct gtaccgatag cagctgccat aagactgcta tgctgacttc tttctcctaa
     9001 aggaatcttt ctttgaccaa gtgtgaggat acatgctaaa gagccgaatc cagctgtcgt
     9061 gtggatgact gctccaccag cgaaatctct aaatcccatc tgatccaaaa atccgccacc
     9121 ccatatccag tggcaaacag ggaaataaat cagcagattc caaaaaataa caagtataat
     9181 atagcctttt aaattcaatc tccctgcaaa agctcctgtc atcaaaggaa cggtaatcac
     9241 gataaacatc agttggtaaa gaaagaacat taaaaatgga atcgttgcac catgaaacat
     9301 gttggggaag cttgtaacat gtctcatcaa aaagaaatct gccgggtttc caataacacc
     9361 gccgatatcc cgtccaaagg ccaatccgaa tccaccaaat acccacataa tcgttgttat
     9421 cccaacggag agaaaagatt gagacatgat cgttagtata ttattcttcc gtgctaatcc
     9481 gccgtaaaaa aaagctagac caggagtcat caggcaaacc attgctgtac ataaaatcat
     9541 gaatgctgta tctcctgtat tgataggtaa tgtattcatt tttttcgcct cctttctacg
     9601 attacgagta taaaagaagg tgtagttgtt tttttcttct gtttttttga attaatactt
     9661 gcatttataa aagacagaca attaataaaa gcttgatgtg ttgtgctctt tttcgttttt
     9721 cacacaaata aagacaaaaa ataaacggtc ttctttttat agaaaaccgt ttattttcgt
     9781 ctttttttat ttttaaagat attttttcat aaggaataaa
//
LOCUS       DARBET010000088         9704 bp    DNA     linear   BCT 07-NOV-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4027
            SAMEA114569841-rid20112183.denovo.090, whole genome shotgun
            sequence.
ACCESSION   DARBET010000088 DARBET010000000
VERSION     DARBET010000088.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA114569841
            Sequence Read Archive: ERR12203666
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 9704)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9704)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001972618.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001972618.1
            Genome Coverage       :: 132x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/07/2023 13:18:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,041
            CDSs (total)                      :: 2,975
            Genes (coding)                    :: 2,908
            CDSs (with protein)               :: 2,908
            Genes (RNA)                       :: 66
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 67
            CDSs (without protein)            :: 67
            Pseudo Genes (ambiguous residues) :: 0 of 67
            Pseudo Genes (frameshifted)       :: 26 of 67
            Pseudo Genes (incomplete)         :: 28 of 67
            Pseudo Genes (internal stop)      :: 20 of 67
            Pseudo Genes (multiple problems)  :: 6 of 67
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9704
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA114569841-rid20112183.denovo.090"
                     /isolate="VRE4027"
                     /db_xref="taxon:1352"
                     /country="Denmark"
     gene            826..1737
                     /locus_tag="SCJ86_002440"
     CDS             826..1737
                     /locus_tag="SCJ86_002440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347566.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication-relaxation family protein"
                     /protein_id="HEG1169957.1"
                     /translation="MKYLLHGYFIDYSTRNSSTLAILCLLHELRVVTRKQLYKLLNFD
                     RSISKQGVTNALVRLYEKQLIDRKKYGTEMYYYLTKEGHQSIGGYYTLPKVPEYNLNH
                     HLQINNYLIKGMALAKDHPHLKRIISERRTVYETKDLNPKTKGRKFFVPDFIFRFRDK
                     ENHEINWSFEIELTLKTRRRYREAIFPKYIRELRTRPESHLIYVTPSGAIQEELHAFK
                     RIFFKRETEKDASQQELFERLHIFSALEFEEGLKALLENDPAVNWRTVEREKIYPNGA
                     VQSRLNLFEESTENEEKPKVKFTPEVL"
     gene            2224..2490
                     /locus_tag="SCJ86_002441"
     CDS             2224..2490
                     /locus_tag="SCJ86_002441"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001245899.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEG1169958.1"
                     /translation="MTTKNEQLKEQARKELQERGLIIEGSFEGDFETYIGCYARPINK
                     PTALDPMNEQEVKEQEKHAVNGFPQDFTEWYEWEINNGKLENFL"
     gene            2562..2903
                     /locus_tag="SCJ86_002442"
     CDS             2562..2903
                     /locus_tag="SCJ86_002442"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347568.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEG1169959.1"
                     /translation="MAKNTPYQVGDIFYSSWGYEQTNVTFWQIVKLTEKTAWFQPIQK
                     KTVEHYSSMSGKVVPMKDKFIEYPLAKTKDSIVKKRIDPETYPDMLCGYRSWDTFHRY
                     KNQAVYESSYY"
     gene            2958..3377
                     /gene="ssb"
                     /locus_tag="SCJ86_002443"
     CDS             2958..3377
                     /gene="ssb"
                     /locus_tag="SCJ86_002443"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded DNA-binding protein"
                     /protein_id="HEG1169960.1"
                     /translation="MMNSVNLVGRLTKEVDLKYTPKGNATGTFILAVNRNYTNANGER
                     EADYIRCVIWRKAAETLAKYTGKGSLIGINGRLQTRSYQNQQGQTMYVTEVLVTDFYL
                     LESKEINEQRSKSMANNQNNSSIPGNEIQISDSYLPF"
     gene            3454..3750
                     /locus_tag="SCJ86_002444"
     CDS             3454..3750
                     /locus_tag="SCJ86_002444"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631630.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEG1169961.1"
                     /translation="MKIIDSYDQRTMQYLGSFDYTDENIINYVASLPPYQSVRLVDFA
                     SDELVLTTMGNFLDYVGDKKWLEKILSKLIAKQTGEEPINAIQLFNRYEQENND"
     gene            3765..4196
                     /locus_tag="SCJ86_002445"
     CDS             3765..4196
                     /locus_tag="SCJ86_002445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347572.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA/RNA non-specific endonuclease"
                     /protein_id="HEG1169962.1"
                     /translation="MKRLKRSIFIVLALIDFVGTGCEAIDVQKAPLSEIKQAITKQDE
                     EQPLNTQVEIVNQNKPSFSEEDFSLENGTRQFFSDLDRLNRVGVASALLHKSRMPTEE
                     RDRLYIKPTGWKQKKMKNGDYLYNRCHLIGYQRATSFPISM"
     gene            4378..4683
                     /locus_tag="SCJ86_002446"
     CDS             4378..4683
                     /locus_tag="SCJ86_002446"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347573.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEG1169963.1"
                     /translation="MKLDMPGRYKLLRVRLFLIKLTNLRMYGSITDLYSKDIRLRMQR
                     TLSKSKNVGHEIRDGRLAVYLSRVGKTCLIDSDESGNATKRFTEEIEQLAELNIRYF"
     gene            5087..5575
                     /locus_tag="SCJ86_002447"
     CDS             5087..5575
                     /locus_tag="SCJ86_002447"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347574.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin family protein"
                     /protein_id="HEG1169964.1"
                     /translation="MKKTIFVLAIFICFGLLLVGYLLFNSNEMRTISTNDLLKKSELK
                     DKKKESLAFIMFTQEECEYCIAINPIIDSVNKETLNKIKKYDISVDFQSKQKCKIKFV
                     PTIVAYSKNKEVGRIDNELDNLYRKKQDGSIVIDKKKTKTLLIDWMKKMDKQGEYLYG
                     ED"
     gene            6909..7211
                     /locus_tag="SCJ86_002448"
     CDS             6909..7211
                     /locus_tag="SCJ86_002448"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347577.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HEG1169965.1"
                     /translation="MTKLGITLDDNIYDEKILLSGGEKQKISLARALIKSASVIILDE
                     PFRNLDNETRDNLRDYILNTKKEVCYIIITHDHILDNFLDSNDEILYLNKEYDNNE"
     gene            7204..8232
                     /locus_tag="SCJ86_002449"
     CDS             7204..8232
                     /locus_tag="SCJ86_002449"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347578.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HEG1169966.1"
                     /translation="MNSKKLQMGEIVRGPSQISIDVTYKCNFRCKHCYNASGGDNCFM
                     DNELTDDELLELTKDICNLKPYNICFCGGETLLRKKIVLSCATLLKEAGIPNIAIVTN
                     GSLVTQDVAKELFKAGINRFQVSLDGKNASSHEALRLYPNSFEKAINAIKLFRELSEE
                     VDVAFCPTSFNYNQLREVYQLVNTLKVKSLRIQPFMLSGRGMQNREIVPTVSQYRKLV
                     SDINYIKRNEDYTKIDWGDPIDHIIRHRTLLKDFSNFVSIRSDGGLMASPYLPIQVGN
                     IKCHSLSKYWRAGLYKVFSLDLVQDIATQVSSIQDMSKELKKGHIKWLNEDIEIDILD
                     EYKHDILI"
     gene            8288..8506
                     /locus_tag="SCJ86_002450"
     CDS             8288..8506
                     /locus_tag="SCJ86_002450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347579.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEG1169967.1"
                     /translation="MISEPQMNNMYESPTIMGAGEAAPRACSAIMVCVLLASAGVGIQ
                     AGAVYTYAVVATVAVAAAAVWKTVGLVG"
     gene            8577..9392
                     /locus_tag="SCJ86_002451"
     CDS             8577..9392
                     /locus_tag="SCJ86_002451"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347580.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEG1169968.1"
                     /translation="MKYNNLKPKLLNENIEYIREENYGYLTIVPKNGTELKDNVFNPT
                     VRYILANCTGKNSVKKIANGLKKKFPSLEYEKFYQDVVGTIKFFKHYNIIDIGDYSME
                     EKKIYKIEQNLELELLNESHYEEIYNFITDHPESMYFHEDIYHQGNSKELEVVLRYNL
                     FSFTSEFYFLRHKERIVGMVSIIVNPFQNLSYSIVNYLICEPAYTTTILTTVLENINE
                     VSILEVKKLVIHLLNSTEDESLIPYLKDSHFFKEAVLSKEYREHDVLRYAYIY"
     gene            9456..9641
                     /locus_tag="SCJ86_002452"
                     /pseudo
     CDS             9456..9641
                     /locus_tag="SCJ86_002452"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347581.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="SdpI family protein"
BASE COUNT         3652 a         1255 c         1819 g         2978 t
ORIGIN      
        1 ttttttcgat cgtatttgtt tttcttttga aacaaattca ctcatttttt gagcaaaaat
       61 gtttcaaaat ttgaacaaaa accaattgaa aattgaagca aatttcactc gaattgaatc
      121 gattttgagc taaaatcaat agaaaaaata aacgtatttt gtttgaaatc cgtttatttt
      181 ttttacgttt cagtattagt aaggattatt tgagggttat ttagagatta tttaaaggtt
      241 atatgtaata agtgggggga gggctgtcgg cgcttggtat gaaaggcatt ctccgacttc
      301 caggtttttg accattttct ctcaacgtgg aaaaaccgcc acttatgaga gaaaatggtc
      361 aaaaacctaa gcgggaagtc tccgaataac ataccaagcg ccgacagcca acagataaag
      421 aatacactac taaaaatagt agtagcataa aatttaaaag ggaaaaaatc aacccctacc
      481 ccagtcttgg ggtctcaaaa ctaactcccc cctaaccccc tctttcagcc tcttttgaaa
      541 gagggggaga atcaacccca cggccaacgc cccagtgttc ttggggctgt ccagaacaga
      601 aacttgcgag agagtggggg taaaacaagt gtagaggaaa cttgaagact tgttcgaaca
      661 gaaacttgcg agagagttgg agtaaaacaa gtgtagagga aacttgaaga cttgttcgaa
      721 cacaaactta caagagaggg gagtaaagca gttgcagagg aatcaggaag atttgttcga
      781 acagaaactt gcaagaaagc attattaaat tgaaggtgat gaaatatgaa gtatttattg
      841 catggctatt ttattgatta ttctactcgc aatagtagta ctttagcgat tctatgtttg
      901 cttcatgaat tgagagtggt tactagaaaa cagctataca aactattgaa ttttgacaga
      961 tcaattagta aacaaggagt aacgaatgct ttagtgagat tatatgaaaa acagttaatc
     1021 gatcgaaaaa aatatggaac agaaatgtat tattatttga cgaaagaggg acatcaaagc
     1081 ataggtgggt attatacgtt gccgaaagtt cctgaatata atttgaatca ccatttacaa
     1141 attaacaatt atttgattaa aggaatggca ttggcaaaag accacccaca tttaaaacgg
     1201 attatttcag aaagacgtac cgtctatgaa acgaaggatt taaatcctaa gacaaaagga
     1261 agaaaatttt ttgtgccaga ttttattttt cgttttcgag acaaagaaaa tcatgagatc
     1321 aattggtcct ttgaaattga gctaacgttg aaaacacgtc gtcgctaccg tgaagcgatt
     1381 tttccgaaat atattcgtga gttgcgaaca cgtcctgaat cacatttaat ttatgtgaca
     1441 cctagtggcg caattcaaga agaacttcat gcgtttaagc gtattttttt caagcgcgaa
     1501 actgaaaaag atgcgagtca acaagaattg tttgagcggt tgcatatttt ttcagcttta
     1561 gagtttgaag agggattaaa agcattatta gagaatgatc cagcagtcaa ttggcggaca
     1621 gtagaacgag aaaaaattta tccgaatggc gctgttcaaa gtcgtttgaa tttatttgaa
     1681 gaatcaactg aaaatgagga aaaaccaaaa gtgaagttta cgcctgaagt attgtaaaag
     1741 agggtgttcg cttcgctctt tttttgtgcc agtttgctgg caccgttcag acgcaagcgt
     1801 ctgaaccggc tcgtttttag gaaggactaa cttgtttgat ttatccgttg tcaaagaagt
     1861 ggacgttcga gagtccctaa tcgtatagca gtcattcggc gagtataacc taaaacataa
     1921 cctgccaatt gccaagggta aaatgcacgc tgcgcgccct tggcaatcgt cagaacatgt
     1981 tttcgagccg gttatgccga acgaccgcta tacgaaaagg gcctctaaac agaagtccac
     2041 tttgatcctt gacaactgaa acccaaaatc aaacaagcag gccttcgtct aaaaacgagc
     2101 cgaatgaaga ccttgggggt tgcaccccca aacccccgtg cccccgacac actaccgaaa
     2161 aggtgggggt gggtggcgaa aaaatcaaat aaaacattct attcaagaaa ggaagaaaca
     2221 accatgacaa ctaaaaacga gcaactgaaa gaacaggcaa gaaaagagtt gcaagaaaga
     2281 ggtttgatta ttgaggggag ctttgaagga gattttgaaa cctatattgg ttgttatgca
     2341 agaccgataa acaaacctac agccttagac ccaatgaacg aacaagaagt aaaagaacaa
     2401 gaaaaacatg cagtgaatgg ttttccgcaa gatttcactg aatggtatga gtgggaaatt
     2461 aacaacggga agcttgaaaa cttcttataa aagtcagatg acgtgcaaag agaatgattc
     2521 tctttgtacg gtaaaaatat aaagaaagaa ggaaaaagaa aatggcaaaa aacacacctt
     2581 atcaagtagg agacattttt tatagtagtt ggggctacga acaaacgaac gtgacttttt
     2641 ggcaaattgt gaagttaaca gaaaaaacag catggtttca gcctattcaa aagaaaacgg
     2701 tcgagcatta ttcgtccatg agtggaaaag ttgttccaat gaaagacaag tttatagagt
     2761 atcctttagc aaaaactaaa gacagtattg taaaaaaacg aattgatcca gaaacctatc
     2821 ctgatatgtt atgtggttat cgtagttggg acacgtttca tagatataaa aatcaagctg
     2881 tttatgaaag tagttattat taaggggata gtttcccctt tcaaaaataa taaatagaag
     2941 aatggagaga aaaaatcatg atgaattcag tgaatttagt aggacggtta acaaaagaag
     3001 tggatttaaa atacacacca aaaggcaatg cgacaggcac atttatttta gctgttaatc
     3061 gaaattatac gaatgcaaac ggcgagcgtg aagcagatta tatccgctgt gtcatttggc
     3121 ggaaagctgc ggaaacttta gcaaaatata ctggaaaagg aagtttgatt ggcattaatg
     3181 gacgtcttca aacgaggagc tatcagaatc agcaaggaca gaccatgtat gttacggaag
     3241 ttctagtgac agatttctat ttgttagaga gtaaagaaat caatgagcaa cggtcaaaat
     3301 caatggcgaa taatcaaaat aattcttcaa ttccaggaaa tgagattcaa atttcagata
     3361 gttatttgcc cttttaagtg aaaaataatt ttgttctata cctgatttct tcaggtatag
     3421 aacaaaaaat aaatagagat gaggcgattt tttatgaaaa tcattgacag ttacgatcaa
     3481 cgaaccatgc aatatttggg ctcgtttgat tatacagatg aaaatattat taattatgtt
     3541 gcaagtttac ctccttatca aagcgttcgc ttggtagatt ttgcaagtga tgaattggta
     3601 ttaacaacga tgggcaactt tttagattat gtcggagaca aaaaatggtt ggaaaaaatt
     3661 ttgtctaaat tgattgcgaa acaaacagga gaagaaccca taaatgcgat tcaattgttc
     3721 aatcgatatg aacaagaaaa caatgattaa agaggagaga aaaaatgaaa agattaaaac
     3781 gaagtatttt tattgtgcta gcattgattg attttgttgg gacaggttgt gaagcaattg
     3841 acgttcaaaa agctccctta tcggaaataa aacaagcaat aacaaaacaa gatgaagaac
     3901 aaccgctaaa tacacaagta gaaatagtga atcaaaataa accgagtttt tctgaagaag
     3961 atttttcgtt agaaaatgga accaggcaat ttttttcaga tttggatcgt ttaaatcggg
     4021 taggtgtagc aagtgcactt ttgcataaaa gcaggatgcc aacagaagaa cgagaccgat
     4081 tatacattaa gccaactggc tggaaacaaa agaaaatgaa gaatggcgat tatctttata
     4141 atcgttgtca tttgattggc tatcaacgtg cgacaagttt ccccataagt atgtaatacg
     4201 aaacgggagg attaatctta agattaatca caactaacct atttgacagt tggaatgaaa
     4261 gctatcatgt ctgctgaatc aactggtctg atactcctac atgcaataaa gtctactagg
     4321 aagttattgt atgaagctag gtaaaggcgg cagaacaatt tctaagtgtg gaatcatatg
     4381 aagttggata tgccgggacg ctataaatta ctcagggtga gactgttttt aattaaactg
     4441 acgaacttgc gaatgtacgg gtctataacc gacttatatt cgaaagatat aagattgcga
     4501 atgcaaagaa cattatcgaa aagtaagaat gttggtcatg aaattcgaga tggaagacta
     4561 gcggtgtatc tgtcaagagt tggtaaaaca tgtttgattg attcagatga gagtgggaat
     4621 gcgacaaaac gttttacaga agaaatagag caactagcag aactgaatat acgatatttt
     4681 taaatacttt agcggagaat aaaaaaatcc tctacagaaa actacggagg gcaacatgtg
     4741 ctattgtttc aacaatacat ttttaatttt taccataaca aaaaagtact tgaaaaaaaa
     4801 cagaaatatg ttaaattaaa aatgaaaaaa tggaagcggt tacttattct tttgaatcta
     4861 agataaaaat tcaaaagaat aacataaccc aaatatgatt atacttgaga ttctaaatat
     4921 ttatcgaaaa ggaatttgtt ttaaggaaaa atgattctat ggacggttat tgatttatat
     4981 ttaccaaatg gctaataaaa tagtatttct tatattgtat agtttttgtt tttatgtgaa
     5041 aacatgagca atagaattgt actagtagga taaggatgga tggaaaatga aaaaaactat
     5101 ttttgtttta gcgattttta tatgttttgg gctacttttg gtaggctact tgttgtttaa
     5161 ttctaatgaa atgagaacta tttctacgaa tgacttgtta aaaaaaagtg aattaaagga
     5221 taaaaaaaaa gaatctcttg cctttataat gtttactcaa gaagagtgtg agtattgtat
     5281 agcaataaat ccaatcatag atagtgtaaa taaggaaact ttgaataaga taaagaaata
     5341 tgatatatct gtagattttc aatctaaaca aaagtgtaaa attaagtttg ttccgactat
     5401 tgttgcatat agtaagaata aagaagtggg gagaattgac aatgaattag ataatttgta
     5461 tcgaaaaaag caagatggtt caattgtaat agacaagaaa aaaacaaaga cattgcttat
     5521 agattggatg aaaaaaatgg ataaacaagg agaatatttg tatggagaag attaaacaga
     5581 catttccgtt aattaataaa tattggaata agaaaaaaat ttttatattt tttattacgt
     5641 ttctgctgtc atctttattt tctctaattt tcccgtatct atttagtaat gttattgatt
     5701 tatatttaga gaatgagtta tcacagaata ttgtgcgaat agtaacgatt cttatatttc
     5761 agttattttt agtcttaatt aaagaagtgt atggatatat aaaaactatt tatgcatttg
     5821 aagttgattt atcaatgaaa attgattttc ttaaggtttt acaaaaaaaa aatctttttt
     5881 tttcgcaaac gaagcattca ggagaaattc aatttaggat gttttcggat atttctacta
     5941 tcgtaaatac tatctttatt ttttctgttg aattacctgt gataggcgtt ttagttcttg
     6001 tagcggtatt tattataagt aaagtgaatc ttatttttct gattttttca ctaataatga
     6061 tgggagctat ggtgttacag tataatttat ttcaattacc gcttaagaaa acaatcgaga
     6121 atacaaaaag tgttaatcaa agtattacag gagaattgaa tgcacatttt gatcaattag
     6181 aaattataag cgcatataat tttgagaata tctctatttt aaaattcgat tctagattta
     6241 aagaattatt taatgttttc aaaaaacaaa ataaattact aacactggga agctgtttgg
     6301 cacaggttat tttccaatta tggattggag gaatatttct tattgatgtt attttattag
     6361 ctaaaggaac aataagtatt actaattttg taatagtatt tatgtacgct aacatggtcc
     6421 ctactttatg gactacttta attagtatta tttggaaatg gcaagactat tcagtgtcat
     6481 taaatagata ttacgaatat tattcaaata acaacaaagt ggctggcaca aaaaaaataa
     6541 ataaaataaa ttctattact ggtcaatcat taaatttttc atataacaaa agaaaggtat
     6601 taagcaatat aagtttttca actgaaatga ataaactatt catgatatca ggagaaaatg
     6661 gaacagggaa aacaacactg atgaaattat ttactaaaca gttgccggtt agtaataatc
     6721 aattattcgt caacgggata gatattaata tgattgataa cgaatcatta agaaggttaa
     6781 tttcaatcgc aacacaaata ccatatataa ttaatagctc cgtgaaggag aatattttaa
     6841 tggggactaa aaactatgat aaaaaaaaat tcagtaaatt aatagaacta ttgggaataa
     6901 gtaaatttat gacgaaatta ggtattactt tggacgacaa tatttatgat gaaaaaatat
     6961 tattatctgg aggagaaaaa caaaaaataa gtttagctag ggcgcttatt aaatcagctt
     7021 cagttattat attagatgaa ccatttagaa atttagataa tgaaactcgc gataatttga
     7081 gggattatat actaaataca aaaaaagaag tttgttatat aattataact catgatcaca
     7141 ttctagataa ttttttggat tcaaatgatg aaatattata tttaaataag gagtatgata
     7201 ataatgaata gcaaaaaact tcagatgggt gaaattgtta gaggtcctag tcaaatatct
     7261 attgatgtta catataaatg taattttaga tgtaaacatt gttacaatgc gagtggtgga
     7321 gacaattgtt ttatggataa tgagttgact gatgatgaac tgttagaact tacaaaagat
     7381 atatgtaatt taaagccata taatatttgt ttctgtggag gagaaacttt actgagaaaa
     7441 aaaatagttt tatcttgtgc cactttatta aaagaggcag gtattcctaa tattgctatt
     7501 gttactaatg gatcattagt aactcaggat gttgcaaaag aattattcaa agcaggtatt
     7561 aatcgatttc aagtaagttt agatggaaaa aatgctagta gccatgaagc acttagatta
     7621 tatccaaaca gttttgaaaa ggcaattaat gctatcaaat tatttagaga attgtccgaa
     7681 gaagtggatg ttgctttttg tcctactagt tttaattaca atcaattacg agaagtatat
     7741 caactagtaa atactcttaa agtgaagtct ttaagaattc aaccatttat gcttagtggt
     7801 aggggaatgc aaaataggga aatagtgcca actgtttctc aatatagaaa actagtttct
     7861 gatattaact atattaaaag aaatgaagat tataccaaga tagattgggg agatccaatt
     7921 gatcatatca tccgacacag aactttattg aaagattttt cgaattttgt ttctattagg
     7981 tcagacggag gattaatggc atcaccttac ttgccaattc aagttggaaa tattaaatgt
     8041 cacagtcttt ctaaatattg gcgagcagga ctttataaag ttttttcttt agatttagtt
     8101 caagatatag ctactcaagt ttcttcaata caagatatga gtaaagaatt aaaaaaagga
     8161 catattaaat ggcttaatga agatatagaa attgatattc tcgatgaata caaacatgat
     8221 attttaattt aggtttatca ttggaaagtt tagataaaca aagtactttt agaaaggagg
     8281 gaaaaaaatg atttctgaac ctcaaatgaa taatatgtat gaaagcccaa ctataatggg
     8341 agctggtgaa gctgcgccaa gagcttgttc agcaattatg gtgtgtgtgt tattagcatc
     8401 tgcaggagta ggtatccaag caggagcggt gtatacttat gctgtagttg ctacagtagc
     8461 tgtagctgca gccgcagttt ggaaaacagt aggattagtt ggttaggaat taataggaga
     8521 ctaactgtct cctattgatt ccaatgatta agctaaggag gaattttaag aaaataatga
     8581 aatacaataa tttaaaaccg aaattactta atgagaacat agaatatata agagaagaaa
     8641 attatggata tttaaccata gttcccaaaa atggaactga attgaaagat aacgttttca
     8701 atccaacagt aagatatata ttggctaact gtactggaaa aaacagtgta aaaaaaatag
     8761 cgaatggttt aaaaaagaag tttcctagtt tagaatatga aaaattctat caagatgttg
     8821 taggaactat taaatttttc aaacactata atattattga tataggagat tatagtatgg
     8881 aagaaaagaa aatatataaa attgaacaaa atttagaatt agaattacta aatgaatcac
     8941 attatgaaga aatttataat tttatcacag atcatcctga aagtatgtat tttcatgaag
     9001 atatttatca tcaaggaaat agtaaagaat tagaagttgt ccttagatat aatttatttt
     9061 cgtttacgag tgaattttac tttttaagac ataaagagag aattgtagga atggtttcta
     9121 taattgttaa tccatttcaa aacttatcct acagtatagt aaattacctc atatgtgagc
     9181 ctgcatatac tacgactatt ttgacaacag tattagaaaa tattaacgaa gtttcaattt
     9241 tagaagtaaa gaagctagtg attcatttgc taaattcaac agaagatgaa tcactaatcc
     9301 cctatttaaa agattcacat ttttttaaag aagcggtact atctaaagaa tatagagagc
     9361 atgatgtttt gagatacgct tacatttatt aaataaaatt tctgatagta ttttaataat
     9421 tcggatcata tcataagata taggagggaa aagaagtgat tttattgatt ttgtctctcg
     9481 tattactagt tgtatctaat ctaatgaaaa ataatcctcc tacaaaaatt aattcttttt
     9541 atggctttag aactaaaaag tcaatgaaaa atgaaaaaag ttggcattta gcacaaaaat
     9601 tgttggggga tttttcaaaa aaaaaagtta tcccagtatt tttgatgggt attgtttttt
     9661 tcagtataga gatttatagt ttagctattt taaaaaatga aaat
//
LOCUS       DARBET010000089         9562 bp    DNA     linear   BCT 07-NOV-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4027
            SAMEA114569841-rid20112183.denovo.091, whole genome shotgun
            sequence.
ACCESSION   DARBET010000089 DARBET010000000
VERSION     DARBET010000089.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA114569841
            Sequence Read Archive: ERR12203666
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 9562)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9562)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001972618.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001972618.1
            Genome Coverage       :: 132x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/07/2023 13:18:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,041
            CDSs (total)                      :: 2,975
            Genes (coding)                    :: 2,908
            CDSs (with protein)               :: 2,908
            Genes (RNA)                       :: 66
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 67
            CDSs (without protein)            :: 67
            Pseudo Genes (ambiguous residues) :: 0 of 67
            Pseudo Genes (frameshifted)       :: 26 of 67
            Pseudo Genes (incomplete)         :: 28 of 67
            Pseudo Genes (internal stop)      :: 20 of 67
            Pseudo Genes (multiple problems)  :: 6 of 67
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9562
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA114569841-rid20112183.denovo.091"
                     /isolate="VRE4027"
                     /db_xref="taxon:1352"
                     /country="Denmark"
     gene            complement(354..608)
                     /locus_tag="SCJ86_002453"
     CDS             complement(354..608)
                     /locus_tag="SCJ86_002453"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286933.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEG1169969.1"
                     /translation="MIGKIQSDPELEEELYRLNFDIRKNGVKVPDDPYVIDEETDARM
                     ELNQVISELERIADFAKEPDIRQYLFEIKAELEIEGITAE"
     gene            complement(633..1775)
                     /locus_tag="SCJ86_002454"
     CDS             complement(633..1775)
                     /locus_tag="SCJ86_002454"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286932.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA cytosine methyltransferase"
                     /protein_id="HEG1169970.1"
                     /translation="MTFLDLFAGIGGFCLGMEQAGHQCIGFCEIDDFARQSYKAIHDT
                     SKEVEMHDITSVSDEFIQSLGPVDILCGGFPCQAFSIAGKRQGFSDTRGTLFFEIARF
                     AAILQPKFLFLENVRGLLNHEGGATFETILRTLDGLGYDVEWQVLNSKAYVPQNRERI
                     FLIGHSRDACTEQVFPIIGSSPTSDQNIRNLLNINPSNRGMGGQVYGSDDVAPTLTGD
                     EGIKIALPVDDGISVAGMLLGNFEQGNRVYEITGTAPTLSTKQGGTKIMLRKNETEYQ
                     EVKPGNSVNLAFPNSTSRRGRLGKQSVHTLLTGDQQAVVTDQYQIRKLTPRECWRLQG
                     FPDWAFDRASQVNSDSQLYKQAGNSVTVPVIFDIARRLKEVKTDDL"
     gene            complement(1835..3187)
                     /locus_tag="SCJ86_002455"
     CDS             complement(1835..3187)
                     /locus_tag="SCJ86_002455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002367544.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF87 domain-containing protein"
                     /protein_id="HEG1169971.1"
                     /translation="MYKGHRIRAGDQHLVYHFVLGWLLALFIGWMSVFYFQELRQFDI
                     SKLSLSTIEIVRSIKDLIYLLGSLVLSGSTMLLYIHFFQDHWRSLWHRQKLARMILEN
                     HWYEVKQTQSEGFFKDLNSSRTKETISYFPKIYYRMKDGLLSIRVQISLGKYQDQLLK
                     LEKKLESGLYCELVEKELKDSYVEYTLLYDIIANRIGIDEVVAESGALRLMKNQVWAY
                     DSLPHMLIAGGTGGGKTYFLLTIIEALLKSDAELFVLDPKNADLADLGTVMPHVYSQK
                     EEISACVEDFYERMMTRSKAMKEMPNYKTGENYAFLGLPPNFLIFDEYVVYMEMLTTK
                     ESAVILNKLKQIVMLGRQSGFFLILACQRPDAKYLGDGIRDQFNFRVALGRMSELGYS
                     MMFGEVDKKFFMKRIKGRGYVDTGGSVISEFYTPLVPKGYDFLREISDIGGLNVNTEG
                     "
     gene            complement(3258..3602)
                     /locus_tag="SCJ86_002456"
     CDS             complement(3258..3602)
                     /locus_tag="SCJ86_002456"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330683.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelB/DinJ family
                     antitoxin"
                     /protein_id="HEG1169972.1"
                     /translation="MEINVEHILDCLDQYGKGELTEEQLTKALTYDEKMFLIMHQGLL
                     EVGNDAEEDFDVLNWLGEEESFFMVIEVNESLCREAETVLEEIGVEMPDAIEVFLKQL
                     VETKQLPIAVND"
     gene            complement(3612..3986)
                     /locus_tag="SCJ86_002457"
     CDS             complement(3612..3986)
                     /locus_tag="SCJ86_002457"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdcP family protein"
                     /protein_id="HEG1169973.1"
                     /translation="MRLTEGIVVDSGLTFGKLRFSALRREVRKQNEDGTVSNEVKERT
                     YNLKSSAQGRMIQVSIPANVPLREFAYDAEVELVNPIMDTVANYVFREGTTVNWFIKA
                     DDLVLKRQPDQGNSTNQNEGKK"
     gene            complement(3999..4313)
                     /locus_tag="SCJ86_002458"
     CDS             complement(3999..4313)
                     /locus_tag="SCJ86_002458"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016171806.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdcP family protein"
                     /protein_id="HEG1169974.1"
                     /translation="MELKFVVPDMAETFGKIRYAGEGEVLTEGYGRNTTVIGRSYHLY
                     SSKQRADDIEVVVAAEAGEKDFDQDQPLKAVNPHLVAKGYEIENRGFTDYVLYVDDLV
                     KA"
     gene            complement(4427..7652)
                     /gene="fss3"
                     /locus_tag="SCJ86_002459"
                     /pseudo
     CDS             complement(4427..7652)
                     /gene="fss3"
                     /locus_tag="SCJ86_002459"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286924.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="fibrinogen-binding MSCRAMM adhesin Fss3"
     gene            complement(7731..8393)
                     /locus_tag="SCJ86_002460"
     CDS             complement(7731..8393)
                     /locus_tag="SCJ86_002460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010740290.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEG1169975.1"
                     /translation="MKQNDGRIIWKNQSELKLILTINEFIEKHGITSSRQYQKKLSEN
                     PNSAPSMWFINKKYGSWENLLISIGRENTGYGKWARMSEQELLEIVEAFIKCEKITSQ
                     RMYEQKSVGKNIPSLSTIKKMLGDIRPLFKEKNDGSRFTDFELLLELKNEIIRLKLQD
                     DLSMTKFRKLVQSPKLPSVDTIMKRTNKNWEELMAEIGFDYRRIKIYKQRNNLSKTKK
                     TK"
     gene            9010..9240
                     /locus_tag="SCJ86_002461"
     CDS             9010..9240
                     /locus_tag="SCJ86_002461"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002378238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEG1169976.1"
                     /translation="MMEDFIRKNISDEYADFYEQSSKKDKFQMDVSILAILAFSENKQ
                     PVTAKKETVFSEGKIKTRYILEVETNFKNRSE"
     gene            9246..9545
                     /locus_tag="SCJ86_002462"
     CDS             9246..9545
                     /locus_tag="SCJ86_002462"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEG1169977.1"
                     /translation="MLFNETQLWFKFDPTNRFIKDFYKVWNSEVFFLAIEDSLLINLY
                     YSNKNYFKIPAAKTRMKKGVYFLFDIVTDVPDARSDHRRYDYIKYTFVDPERYKD"
BASE COUNT         2648 a         2090 c         1627 g         3197 t
ORIGIN      
        1 cgactaagtc cacaagtcgt agctaattta ttttgtgaaa cgccatattc attacgccgg
       61 tcttttaata gccgataatc taaattcctt tgtgccaagt tctcacctcc aaatttaagg
      121 tgttaacttt ttcaaatcag ttaacatcta cacaaattgc ttattatcct tgataggaca
      181 gccattcctt atcgttgctt gcttattttt tgtgtttttg tacccccctg ttagatacgg
      241 ggggtgagac atcatggccg gctaacgccg tccataaaca ggcacagcac cacggaggat
      301 ttctgcttcg caaatccccc gcggtcagtg cctatcagtg gtttatttgg ccgttactcc
      361 gcggtaatac cctcaatttc caactctgct tttatctcaa acaagtattg gcggatatca
      421 ggttctttag caaagtccgc aattcgttcc aattcactga tgacttgatt cagctccatt
      481 cgagcatccg tttcctcatc aattacatac ggatcatcag ggactttcac cccgtttttg
      541 cgaatatcaa aattgagccg gtacagttct tcttccaatt ccggatctga ctgaatcttg
      601 ccaatcaccc agtagattcc ccaatcaact catcataaat catctgtttt tacctccttt
      661 aatcttctag caatatcaaa aatgaccgga actgttacag aatttccagc ctgcttatac
      721 aactgactat ctgaattgac ttgagaagct cgatcaaaag cccagtccgg aaagccctga
      781 agccgccagc actccctagg tgttaacttt cgaatttgat actgatccgt gacgactgct
      841 tgttggtctc ctgttaataa ggtatgcaca ctttgttttc ccagacggcc tcgacgactt
      901 gtggaattag gaaaagccag gttcacactg ttccctggct tcacctcttg atattctgtt
      961 tcgtttttcc gaagcatgat cttcgttccg ccttgtttcg ttgacaaagt aggcgctgtt
     1021 ccagttatct cataaacccg attgccttgt tcaaaatttc ccagtaacat acccgctaca
     1081 gaaattccat catctaccgg caatgcgatt ttgattcctt catcaccggt caaggtaggc
     1141 gctacgtcat ctgacccata gacttgtccc cccattcctc gattggaagg gttgatattt
     1201 aataagtttc tgatgttttg atcagatgtt ggagacgatc caatgatagg aaatacttgt
     1261 tcggtacatg cgtctcgaga atgtccgata aggaagatcc gctcacggtt ttggggaacg
     1321 taggcctttg agttaagcac ttgccattcc acatcatacc ccaatccatc cagcgttcgg
     1381 aggatcgtct cgaacgtagc ccctccttcg tgattgagta atccccggac gttctcaagg
     1441 aataaaaact taggttggag aatagcggcg aaccgagcga tttcaaagaa taaagtacct
     1501 cgagtatcgg aaaatccttg ccgctttccc gcaattgaaa aagcttggca cggaaatcca
     1561 ccgcaaagga tatccactgg tccgagagat tgaataaact cgtctgatac acttgtgatg
     1621 tcatgcattt ccacctcctt acttgtatca tggattgcct tataactctg gcgagcaaaa
     1681 tcatcaattt cacaaaagcc gatacactgg tgacccgctt gttccatacc taagcaaaag
     1741 ccaccaatcc cagcgaataa atctaaaaat gtcataaatc acctcgtaaa aaaagcccag
     1801 gattcttgac actcctaggc tacatactat tattttatcc ttccgtatta acgtttaatc
     1861 caccaatgtc gctaatttcc cttaagaaat catatccttt tggtacaagt ggcgtataaa
     1921 actcactaat cacactccct cccgtatcta catagcctcg acctttgata cgcttcataa
     1981 agaatttttt atcaacctcg ccaaacatca tggaatatcc cagttcactc atacgaccca
     2041 aagccacccg gaaattaaat tgatcgcgaa tcccgtctcc taaatatttc gcatccggtc
     2101 tttgacaagc tagaatcaga aagaaaccag attgacgacc taacatcacg atttgtttga
     2161 gcttattcaa aatcaccgca ctttccttcg tcgttaacat ttccatataa accacgtatt
     2221 catcaaagat taaaaagttt ggtggaagtc caagaaacgc ataattctct cctgttttgt
     2281 agttgggcat ttctttcatt gccttgctac gagtcatcat gcgctcatag aaatcttcca
     2341 cacaagcaga aatttcctcc ttttgggaat agacatgagg cattaccgta cctaaatcgg
     2401 ctaagtccgc atttttggga tcaagaacaa aaagctcggc atcagatttt aataaagcct
     2461 cgatgatagt gagtaaaaag taagtcttcc caccacctgt gccgccagcg attaacatat
     2521 gaggtaaaga atcataagcc catacttgat ttttcatcaa tcgcaaagcc ccactttctg
     2581 ccactacttc gtctatccca attcgattgg caattatatc atacagtaaa gtgtactcca
     2641 cataggagtc tttgagttcc ttttctacta gttcacaata tagaccactt tctaacttct
     2701 tttccagctt taaaagctga tcttgatatt ttcccagtga aatttgaacg cgaatagaaa
     2761 gtaaaccatc cttcatacgg taatagattt tggggaagta actgattgtc tctttggttc
     2821 gactactatt gaggtctttg aaaaatcctt cactctgagt ttgttttact tcgtaccaat
     2881 gattttctag aatcatccgg gctagctttt gtcgatgcca caaactgcgc caatgatcct
     2941 gaaaaaagtg tatataaagc aacatcgtgc ttcctgaaag aacgagactt cctagcaaat
     3001 agattaaatc ttttatagaa cgaacgattt ctatagtaga aagtgatagc ttagaaatat
     3061 caaattgtct aagctcttga aaataaaaga cgctcatcca accgatgaac agagcgagta
     3121 gccagccaag aacaaagtga taaaccaagt gctgatctcc tgcccgtata cgatgccctt
     3181 tgtacatttt tatggtttcc acctccaatc gaaaaaagct tcataggatt tcattgatcc
     3241 aatttcaagc agttatgcta gtcatttact gctataggga gttgtttcgt ttccactagt
     3301 tgctttaaaa aaacttcaat ggcatccggc atttccacac caatttcttc caatacggtt
     3361 tcggcttctc gacatagact ttcatttact tcgataacca taaagaagga ctcttcttcc
     3421 cctaaccaat tcagtacatc aaaatcttct tcggcatcgt tacctacttc cagtaaccct
     3481 tgatgcataa tcagaaacat tttttcatca taggttaatg ctttagtgag ttgttcctcc
     3541 gttagctcac cttttccata ttgatcgaga caatctaaaa tatgttcaac attaatttcc
     3601 atacgcttca cctatttctt tccttcattt tggtttgtag aatttccctg atctggttgt
     3661 cgtttcaaga ctaaatcatc ggccttaatg aaccaattga cagtagttcc ttctcggaaa
     3721 acataattgg cgaccgtatc catgattgga ttgaccaact ctacctctgc gtcataggca
     3781 aactcgcgta agggtacatt tgctgggata ctaacttgga tcattcgacc ttgcgcagag
     3841 gatttcaaat tataagtccg ttcttttacc tcgttactaa ccgttccatc ctcattttgc
     3901 ttccgtactt ctcggcgtaa tgcggaaaat cgtaatttcc caaacgttag tcctgaatct
     3961 acaacaatac cttctgttaa tctcatcttc ttttcctcct atgctttcac taaatcatcg
     4021 acatacaaca cgtagtcggt aaacccacga ttctcaatct catagccttt ggcaaccaaa
     4081 tggggattca cggcttttag tggttgatct tgatcaaaat ccttttcgcc ggcttccgca
     4141 gctaccacca cttcaatatc atcggctcgt tgcttactgg aatacaaatg ataactacga
     4201 ccaattactg tagtattccg cccatatcct tcagttaaaa cttcgccttc gccggcatac
     4261 cttatctttc caaaggtttc ggccatatct ggcacaacaa atttcaattc catcttcttt
     4321 tcctcttttc atttttagtt ttggatacaa aaatagcgcg accacttttt ttaggaggcg
     4381 gtcgcgcacg ttggtttttc ctatttagtt gtttcatcac atcaccttag tttcggcgat
     4441 tccagaaata atacccagca gtcgcaaaga tcaaggtaaa cccaataaac agcagaacat
     4501 tagaattttt ctcccctgtt tgtgggaatg tctttactgt aggactagat tcttgagagt
     4561 cacttggaac agttggtatt tccggttgtt ctggtgcact tggtgttgtc ggaacttttt
     4621 taggtgttaa agtttgagtt ttttctttta gatcctcatt gtgtttccca ttgatgtttc
     4681 cgtctttgcc atagttaatt tctttgaagg taaaacttgt accttctgga tatttacttg
     4741 tgtccacttt ttccgcaagt acggttttag tgaattcttt gtcattcact tcataatcaa
     4801 ttttgccaga tttccaaatt tctttggtcg tattatccgg aagtaatgca tacagaattg
     4861 tttcaaaagc ttcttttgag ccatccagta catcatgggt aatcgacaca tcatcgaaca
     4921 tatccaccac atccccatga gtaaaggttt gcgaaccatc ttctaggtgg gcttttgttt
     4981 ggatggaaac atggcgttcc accgtacaat tgacagtttg ggcttgattg ttcaagctag
     5041 catctttcgc tactggatca tcacctgctt catagggttt cttgttttca tacacatagt
     5101 tgaataagac gatggtttta tccaaaactt gttctgcggt taatttatgc aaaattttcc
     5161 aagttcctgt tttctcttta ttcgccaacg ttgcggttgt ttcggcaatt accactggtt
     5221 tggctttttc atctttttct tgggcagctt ttgtcgcttc cacatcaatg gcttgcgcta
     5281 ccacatacca gtcttctttg atttcatgca agttatagct caatttatca actagctcgg
     5341 tagaatcaag gtctgtcaat tctttgcctc cgtctttcca agtcccaaga gaagttaagc
     5401 tatcttcttc ttcaggtaaa tcatagagca tcaaacgatt caagctaaca gaaaatgctt
     5461 tattcgtcag tttctcgtaa ggaacggtca ccgttttaat tggttgtttt tgtccttttt
     5521 cggtaatcgt aaaaatgtat tcactcttag tgtaatccgc tttattttct ttaaaagtag
     5581 aacggatttc taatggcgta attttttgaa atccttctgg agcctctact tcttcaagta
     5641 aataatcccc ataaggaaga ttttcaaact tgccatagcc atcaaaacct agttgctcgt
     5701 tataagctgt aattgcttca tcttcggcac ctgtaatctc actggttcct tccaatggcg
     5761 atactttaaa ggtcagatca ttgaagccag tttcggcagt cccagcagct gatccagcaa
     5821 atttaaagaa atcaaagcca aagcgaatga cttgttcttt tgccgtaaca tctcgggtaa
     5881 tcacggcatt tttttcgtta tcatccacct ttttgatgga aacagggtac tttgtttcat
     5941 ccaaggtata tccttcaggt gctttggttt cttgccagta atactcatta atcgctaagt
     6001 gtttaacagc aacttgattc ttttcatcta aagtcaaagt caccgtttca tcagaagctt
     6061 tcgttccctt cactaactct ggtttaaaag cctcactcca tttgaccgct tgaccatctt
     6121 ttgcagtaaa gagagtatat tcagctcctt taaactcagc tttaccttga gtttcattac
     6181 cggtatcttt gtcttctttt gtcaaagtgg tttcaccagt gatttcttgg ttttgccctt
     6241 ttacgttact ggtaacaaga gccacggttt gattgacata gttcaattcg acttttactg
     6301 gtttaaaggt attcacaaag ccattactag ctttggtttc ggtcacataa taggttccca
     6361 attccaacgc attggcaatc tcttttggtg tttccgcatg acctttttca tccgtggtca
     6421 tttcttggac aatttcaccg gttggactgt ctttgcgaat ggcaaacgta tttccggcta
     6481 atgagtattt gtcgttccaa agatcagttc ctgtttccac ccccatttta ttcaaaatga
     6541 tttgaccttt ttcgcgagta ttttttgaag tcacagaaat agtctcacca gatttaatgg
     6601 ttgcggtgat tggtgtagtg tcaatcgtat aaggagctgg cactgatttt tcagtaatgg
     6661 ttactttcgc gccatgagga atctcatcca atgtcgcaat gccttcttta tcggtggtca
     6721 cgtcttttgc aggtaatgtt tttccaaagt ctaaatggaa aacggttccc ggtacaacat
     6781 ttcctgtttc tttatcgact tttttaatct tcaaagaacc ctcagtttcc acatcaattt
     6841 tgattgtgta ggtattgggc ttatcaattg cccccgccat caaagtttgt tgaccagcca
     6901 ttttataagc taccggtgtt cctgtattag ctgacttctt tagggttaat tcaccactct
     6961 cttttgaatt ggcatttgga gtaatcacta gttgattgcc gttcacacga taatcaatat
     7021 ttgcgatatt ctcgacaact tcatcaaact ccgataaatt caagccgttc gtatctgtca
     7081 cagtagtcga ttgacctaac acggttttca ctgtggagtt atggaagctt ggtttctttt
     7141 gataatccgc aatggcttga ttgatttttg actcaatggc tgcgatattt actgcactac
     7201 catcagaacg tttaattgag tgaagcgtag ccattaactt cttgccaaat catcttctgg
     7261 gccacaatat gtgtatccac atccgtacca gcttccttcc ataaaacgga aactaatttc
     7321 gctttctccg acatatcagg caatggattt ttctcataac caggagtgaa ctcattgtaa
     7381 atcggcaccc ctggctcaat acagaaaata tcttgcttca caccattgta aactgcatag
     7441 tgcggaacgg taacttctga catgggacga ccatcgctaa atgtaccttc gacagcatac
     7501 tggtgagcca aatccaattc cacagttacc gtttgtggat gagtaatttc atcagcaaag
     7561 gcaatcgttg ggccaagcga tccaccaatt gtttggcata atagactcag tgttgcgagc
     7621 aaacgccaat tcttaaatct tggctttttc ataaaataaa tctcctttcg tttttgggta
     7681 ataaaaaaga cgtccaaatt ttaattttga acgtctgatt tagagtgata ctatttagtt
     7741 ttctttgtct tggataggtt gtttctctgc ttataaattt tgattcttcg ataatcaaat
     7801 ccaatctctg ccatcaattc ctcccaattt ttgttcgttc ttttcataat tgtatcaact
     7861 gatggtagtt ttggggattg aactagtttt cgaaactttg tcattgataa atcatcctgt
     7921 agttttaatc ggattatttc attttttagt tctaacagca gttcgaaatc agtaaatcga
     7981 gatccatcat ttttttcttt aaaaagtgga cgtatatctc ctaacatctt tttaatggta
     8041 ctaagtgaag gaatattctt ccctactgat ttttgttcgt acattcgttg agaggttatc
     8101 ttctcgcact taataaaggc ttctactatt tccaataatt cttgttctga cattctagcc
     8161 cattttccat acccagtatt ttctcgtcct atactaatca ataaattctc ccatgatcca
     8221 tattttttgt taatgaacca catacttggt gcagaatttg gattttctga tagctttttc
     8281 tggtattgac ggctagatgt aattccgtgt ttttcgatga attcattaat tgttaaaatc
     8341 agttttaact cactttgatt tttccaaatg atacgtccat cattttgctt cacgataaaa
     8401 actcctaata gttatttaga tagaagccta tcacatcaac tttttagtag aaaagtacat
     8461 gttttgtctt ttttgctaat agctgaccct ttgagaaact tgggttcaaa agttgctaaa
     8521 ttcggtaaat gttttggtat ttttcgtatt atatttgtcg tcaagctgac tactaaattt
     8581 tgccctattt tgacgacact gatagtcaat gtcggacaat ctaggacaat ttttaaggga
     8641 atatgattga aaaatggtcc aaaacagcca aaaatggctt aaaactgtga tagagaaaaa
     8701 agaaaaagcc cttgatatac aaggactttt cacaaatttc agatttccaa ggaagttttc
     8761 cgtgtttatc ttctgatttc tttgatacga gcagcttttc cgtgtaatgc acgtaaacaa
     8821 aaccactata ttctattatg tatctaatac cctcaaaaca gctataaatc aacaataccg
     8881 acatataata aacactatgt tcaatctgag atatatcaaa gataaacgta tttttgcccc
     8941 ctttttgccc ctcaaatatc gctttacaac gagaacgtaa gttcgtataa tcgttttgag
     9001 gtgattttta tgatggaaga ctttattaga aaaaatatca gtgatgaata tgcagacttt
     9061 tatgagcaaa gcagcaaaaa agataaattc cagatggatg tttcaatttt agctatatta
     9121 gctttttccg aaaataaaca acctgtaact gcaaaaaaag aaacagtatt ctctgaaggc
     9181 aaaataaaaa ctcgatatat attagaggta gaaactaatt ttaaaaacag atcggagtaa
     9241 tggttatgct ttttaatgag acacaattat ggtttaaatt cgatcctacg aatagattta
     9301 tcaaagattt ttataaagta tggaattcag aagttttctt tttagcaatc gaagatagct
     9361 tattaatcaa tctctactat tctaataaga actatttcaa aattcctgct gcgaaaacta
     9421 gaatgaagaa gggtgtatac tttttgtttg atatcgtgac tgacgtgccg gacgcacgta
     9481 gcgatcatcg gcgttatgac tatataaagt atactttcgt tgatccagaa agatacaaag
     9541 attaaataaa aaaataacaa ac
//
LOCUS       DARBET010000090         9339 bp    DNA     linear   BCT 07-NOV-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4027
            SAMEA114569841-rid20112183.denovo.092, whole genome shotgun
            sequence.
ACCESSION   DARBET010000090 DARBET010000000
VERSION     DARBET010000090.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA114569841
            Sequence Read Archive: ERR12203666
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 9339)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9339)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001972618.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001972618.1
            Genome Coverage       :: 132x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/07/2023 13:18:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,041
            CDSs (total)                      :: 2,975
            Genes (coding)                    :: 2,908
            CDSs (with protein)               :: 2,908
            Genes (RNA)                       :: 66
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 67
            CDSs (without protein)            :: 67
            Pseudo Genes (ambiguous residues) :: 0 of 67
            Pseudo Genes (frameshifted)       :: 26 of 67
            Pseudo Genes (incomplete)         :: 28 of 67
            Pseudo Genes (internal stop)      :: 20 of 67
            Pseudo Genes (multiple problems)  :: 6 of 67
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9339
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA114569841-rid20112183.denovo.092"
                     /isolate="VRE4027"
                     /db_xref="taxon:1352"
                     /country="Denmark"
     gene            complement(70..738)
                     /locus_tag="SCJ86_002463"
     CDS             complement(70..738)
                     /locus_tag="SCJ86_002463"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328585.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YitT family protein"
                     /protein_id="HEG1169978.1"
                     /translation="MKKLNLFTELCGIILGAGIYGLAVTGINIPSKLADGGVTGIALL
                     LHNLFGFAPSVTSLLFNLPLLLISLFLFGKHAFLRTIVGTFALVFFLHLWESLHIHFA
                     VDSLLINSLMTGILSGIGCGLVFRFGGSTGGTDIIYQAIEKYFHIKIGKSLFLITFGI
                     LVVSLLYLDLTHFLYTLLSCSILSYTLNKVKYLEIGSVLPQLEAKQTTETFEPLDPLE
                     QLEH"
     gene            872..1159
                     /locus_tag="SCJ86_002464"
     CDS             872..1159
                     /locus_tag="SCJ86_002464"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289767.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEG1169979.1"
                     /translation="MRAADLLTILTKSPKKTEYQGVFLAQEQKLVPVTMVQINKANQF
                     IFLFEHGKAPLAMKDIFLFLMKNREKAVYYQSKEELIPLYGVKEIENKVVI"
     gene            1575..3113
                     /locus_tag="SCJ86_002465"
     CDS             1575..3113
                     /locus_tag="SCJ86_002465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease/substrate-binding
                     protein"
                     /protein_id="HEG1169980.1"
                     /translation="MNNFIQTLSERKGELLEAAFQHLSISLISLLIAALIAIPLAIWA
                     ANHKKMAEVLLQITGVLQTIPSLALLGLLIPFVGIGTVPALIALVIYALLPIFQNTYI
                     GLAEIDPSIEEAAVAFGMSRMRRLLKVELPIALPVIISGIRTALVLIIGTATLAALIG
                     AGGLGTFILLGIDRNTPVLTLIGAISSALLAIIFSGLIRWLQHKKPRYSIITLAAVVC
                     LFGGVSLYQSALFKNETITIAGKLGAEPEILIQMYKELIEDNTKTEVELKPNFGKTSF
                     LFNALDQGQIDIYPEFTGTVLESLVKVPEAMKSADLTKEKTYDEAEELLNKQFDMTLL
                     KPMAYQNTYALAVKRSFANEHQLKKISDLAAISDQIKAGFTLEFIDRSDGYKGIQEAY
                     GLNFSSVQSMEPSLRYQAINNGDVNIVDAYSTDSQLKEYDLVTLEDDRGLFPAYQGAP
                     LMKKDFAKEHPEIVKALNKLSGKITEDQMIDMNYQVNVEKKKPTEVARIFLKNEGLVK
                     EEDQ"
     gene            3110..4075
                     /locus_tag="SCJ86_002466"
     CDS             3110..4075
                     /locus_tag="SCJ86_002466"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315187.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEG1169981.1"
                     /translation="MTKLIEFKHVQKKYDGKYVIDDLNLAIKKGEIFVLVGPSGSGKT
                     TTLKMINGLSKPSAGDIYFKGKSLNEYNLQKMRWNMGYVLQQIALFPTMTVKQNIEVI
                     PEMLGWEKQKRADRVDELLQKVGLSPDIYRDRMPRELSGGEQQRIGIIRAIAASPDVI
                     LMDEPFSALDPISRNSLQELVLSLHEELGTTIVFVTHNMEEAIKLGDRIAFMKDGEII
                     QCDTPEQLLMNPKNDYVRHFFDEPKQTKEWRVEDLVVNGYFLNEIPENARQQVCFDTP
                     MKEVYSLLSVYPSIVIVEKQRSIGSLTSKEIFAFLSREEEGNENI"
     gene            4062..5384
                     /locus_tag="SCJ86_002467"
     CDS             4062..5384
                     /locus_tag="SCJ86_002467"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002372346.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HEG1169982.1"
                     /translation="MKTYDAIIIGSGVSGLSAAYGLKEAGKTVLVVEEDLWGGTCPNR
                     GCDPKKVLLSAVEARNRVKQLSGKGFNEIPTANWEELQKFKRTFTDPVPESRKKQLAE
                     AEIDHLSGTARFLDDSSIEVNEEVFHADYLVLATGQRPTILPVEGKEYLKTSADFLSL
                     PVLPKEIIFIGGGYIAFELATIANAAGSKVTIVHHNQRPLKEFEASLVEEAVHQMEAS
                     GIQFAFGVETQKIISEGTRYRLVGKETELVADMIFCATGRQPNTESLALEQANIVFDK
                     HGIAVNDYLQTSNPKIFACGDIVSRKTPKLTPVATFEGNYVAKRITDATSEPIKYPII
                     PTIVYASPKLAEVGVTKSHASSSDQVVEMDLTSWFTYHRVNEPVAKAELTFDQQNYLI
                     GAAVISEQADELIDDLTLVINQKLTKKELDSYIMGYPTLASDLSYLLK"
     regulatory      5447..5551
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00059"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="TPP riboswitch; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="thiamine pyrophosphate"
                     /db_xref="RFAM:RF00059"
     gene            5625..6368
                     /locus_tag="SCJ86_002468"
     CDS             5625..6368
                     /locus_tag="SCJ86_002468"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342227.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEG1169983.1"
                     /translation="MKKNLYPAVSMAFILIIWQIVVIVWKIPAFILPGPAAVFSALAA
                     DAQVLLSHSVTTLNEAVLGLVIASVLSFITALAMDYWKFLEMSFYPLLVISQMLPIMV
                     LGPLLTLWFGFGMLPKVILVVLISYFPIVVSFSDTLRKVSKEQIIFLKTMGADTGKIY
                     RIYKIPAGLSGFFSGLKVAATYCVSGAVVGEWLSAQAGLGYYMIRVKNSYQIDKVFAA
                     IICVILLSLLLNGACSLLKKGYYRMLYIF"
     gene            6383..7375
                     /locus_tag="SCJ86_002469"
     CDS             6383..7375
                     /locus_tag="SCJ86_002469"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346623.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HEG1169984.1"
                     /translation="MKKLWLLLPLLLLLSACGTAKETSSKQEIKDLKKVTLVLDYVPN
                     TNHTGIYLAKEKGYYKEAGLNVQIIEPGDNSTSIGLVGADKAQFGVSYQEDVTYAHAD
                     GQNIPVKAIATVIKHNTSGFATLSDSNIHSPKDFEGKTYAGWQSPSEEAVLKAVMEKD
                     GGDFSKLTMVGSNGEGPESLGKSSDIQWYFEGWDMIKAKEAGIELNYIPLKELDERLD
                     YYTPVIITNDQLIKSDPELVQSFMDATKKGYQEAIKDPNDSAKLLQKYAKENDRTFLE
                     ESQAFLSKNYTDDPENWGLMEEKVWRNYTAFMQENGLIKQDVPSQELFTNQFIK"
     gene            7387..8124
                     /locus_tag="SCJ86_002470"
     CDS             7387..8124
                     /locus_tag="SCJ86_002470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002390764.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEG1169985.1"
                     /translation="MSIQISHLNVVIENLKILEDIHFTIPKDTFTSIVAPSGAGKSTL
                     LKTLTGVQPMTSGTIKVDDQKVTGLHTAFSYMPQEDMLLPWLSVYQNVTLYQKINHLT
                     INEEQVRDYLDIFGLNGYEHFLPEQLSGGMKQRTALLRTIMNPSSYLLLDEPFGALDA
                     MTRGQMQDWMLKLPKKAKRTTLLVTHDIEEAIYLSDRILVLSARPAHVIAEIQVPEKR
                     RSREWLLQQSELKQTIYQLLAGELDVK"
     gene            8114..8836
                     /locus_tag="SCJ86_002471"
     CDS             8114..8836
                     /locus_tag="SCJ86_002471"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002372354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TatD family hydrolase"
                     /protein_id="HEG1169986.1"
                     /translation="MLSDAHCHLDGSQSLALLQQEHTILTIINCDSPEEWKENRQLAA
                     SKTQALSYGIHPWKADSYTFEQVEPFLKKARIIGEIGLDNIWTNVPMTTQKKVFERQL
                     AFAAINEKPVVLHTKGCEKEILNYIQAYPNHYLIHWYSSLDYQQDYIDLGCYFSIGLD
                     LKKNPAVWQLAKAVPIDHLLIETDGLHAAEWALGGIFSEKDYVSLLEEQLKTIAEIKE
                     LSVEEVKKNTYQNLERFIRLGK"
     gene            complement(8920..>9339)
                     /locus_tag="SCJ86_002472"
     CDS             complement(8920..>9339)
                     /locus_tag="SCJ86_002472"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377182.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase M23"
                     /protein_id="HEG1169987.1"
                     /translation="YSGQQLTIPTEGSAQAAAEPQAAVQEAPVQAEQPVVQETVQTEK
                     QAAPVAETQPAPAVTETAATPASTSSAKEWIAQKESSGSYTATNGRYIGRYQLDSSYL
                     NGDYSAANQERVAEQYVTSRYGSWEAAKAFWEANGWY"
BASE COUNT         3060 a         1607 c         1970 g         2702 t
ORIGIN      
        1 ctgaataata tcattttagt ttacataatg atatttctgt aaattaaata aagtagtgaa
       61 aaataagaat cagtgctcta actgttcaag aggatcaagt ggttcaaatg tttctgtagt
      121 ctgttttgct tccaattggg gcaaaacaga tccaatttcc agatacttca ctttattcaa
      181 tgtataagat agaatagaac agctaagtaa tgtatacaga aaatgtgtga gatccaaata
      241 aagcaaggag acaactaaaa taccaaaagt aatcaagaaa agacttttgc caatcttaat
      301 atgaaaatat ttttcaatgg cttgataaat gatatctgtt ccgcctgtgc ttccgccgaa
      361 tcgaaagacg agaccgcaac caatgccaga aagaatacct gtcatcaaac tattgatcag
      421 tagactatct acagcaaaat gtatatgaag gctctcccat aaatgtaaaa agaaaactag
      481 cgcaaatgtt ccgacgatcg tccggagaaa agcgtgtttt ccaaaaagaa acaatgagat
      541 aagcaataaa ggtaaattaa aaagcagtga agtgacagaa ggagcgaatc caaaaagatt
      601 atgcaaaagc aaagcaatac cagtaacccc accatctgcc agcttagatg gaatattgat
      661 tcctgtcaca gctaatccgt agattcctgc gcctaaaatg attccgcaca attctgtaaa
      721 taaatttaat tttttcattt ctagcctctt ttcttttgaa aattttcagc aacttcttta
      781 ttatactgac ttatactctt acttcaagag gaaaatgaaa aaaaattatt tttggctgtt
      841 tctttttatt tatcaaataa ggagtggaaa aatgcgagca gcagacttat tgacaattct
      901 tacaaaatca cccaaaaaaa cagagtatca aggagttttt ttggctcaag aacaaaaact
      961 ggtgcctgtg acaatggtcc agataaataa agcaaatcaa tttatctttt tatttgaaca
     1021 cggcaaagca ccacttgcta tgaaagacat ctttcttttt ttgatgaaga accgtgagaa
     1081 agctgtttat tatcagtcaa aagaagaact cattcctctt tatggagtga aagagataga
     1141 aaataaagtg gtgatataag taaaaataag aaaacaagac gtttatgatg tcaaaacaac
     1201 cattttttac atttttggca aaaaagtgtc atgatacagt ataatattcg ttgaacaggc
     1261 caataaaggt gttctccccc acgagatttc tactttattg gctgtttctt tttattaaaa
     1321 taaaagttgt gtgtgtaaaa aagatatagc tgattccaag tgacaatttc gtatagattt
     1381 ttatggtatt cgtagtgaaa aaacgagaac caaaaagttg atccgacttt gttgcttgga
     1441 ttcttttttt gtcagcatat ttagaggtat ttttttcttt tttttgttcg tgctgtaagg
     1501 ttaaggcaag acaagttagt tgggaaaaag gaaagaaatg tttatactta aagaataaca
     1561 gaaaggaagg tcgcatgaat aattttatac aaacattaag tgaaagaaaa ggcgagttgc
     1621 tggaagctgc atttcaacat ctgtccattt ctctcatttc tttgctgatt gcagcactga
     1681 ttgctattcc tttagcgatt tgggcagcca atcataaaaa aatggcagaa gtcttattgc
     1741 agatcacggg tgtgctccaa acgattcctt cacttgcctt attaggttta cttatcccat
     1801 ttgtagggat cggaactgtt ccagcattga ttgcacttgt tatttacgct ttgcttccga
     1861 ttttccaaaa cacgtatatt ggtctagctg aaattgaccc gtctattgaa gaagcagcgg
     1921 tagcatttgg tatgtccaga atgagacgct tgctgaaagt agagcttccc atcgcattac
     1981 ctgtcattat ctcaggtatc cgaactgcct tagttttaat catcggtacg gctactttgg
     2041 cagcattgat cggagcaggt ggattaggga catttatttt gttaggtatt gaccgaaata
     2101 cacctgtttt gacattgatt ggtgcgatca gttctgcctt gcttgcaatt atattcagcg
     2161 gactgattcg ctggttgcag cataagaaac ctcgatattc catcattact ttagcagcag
     2221 tggtgtgttt atttggcggc gtttctttat atcagtctgc cttgtttaaa aatgaaacca
     2281 tcacgattgc tggaaaatta ggagcggaac ctgaaatcct gatccaaatg tataaagaat
     2341 tgattgaaga caacacaaaa acagaagttg aattaaaacc gaattttggc aagacgagtt
     2401 ttttgttcaa tgcactggat caaggtcaaa tcgatatcta tcctgagttc acaggtactg
     2461 tcctagaaag cttggtgaag gtacctgaag caatgaaaag tgctgatttg acaaaagaaa
     2521 aaacatatga cgaagcagaa gaattattga ataagcaatt cgatatgaca ttattaaaac
     2581 caatggctta ccaaaatacg tatgccttgg cagtcaagag atcttttgcg aacgaacatc
     2641 aacttaaaaa gatttccgat ttggcagcta tctctgatca gatcaaagcg ggattcacac
     2701 tggaatttat tgatcgttca gatggatata aaggaatcca agaagcatat ggcttgaatt
     2761 tttcaagtgt acaaagcatg gagccaagtt tgcgttacca agcaatcaat aatggagatg
     2821 tcaatattgt ggatgcctat tccacagata gccagctcaa ggagtacgat ctagtgactc
     2881 tagaagatga tcgagggtta ttcccagctt atcaaggcgc accgctgatg aaaaaagatt
     2941 ttgctaaaga acatcctgaa atcgttaaag cactgaataa attaagcgga aaaataacgg
     3001 aggatcaaat gattgatatg aattaccaag tcaatgtcga aaagaaaaag ccgacagaag
     3061 tagcacgaat cttcctgaaa aatgaaggat tagtaaagga ggaagatcag tgactaaatt
     3121 aatcgagttc aaacatgtgc aaaaaaagta tgatggaaag tatgtcatcg atgacctaaa
     3181 tctggcaatc aaaaaaggag aaatctttgt tttggttggt ccttccggta gtggaaaaac
     3241 aactactttg aaaatgatca atggattatc taaaccatca gcaggagaca tctacttcaa
     3301 aggaaaatcg ttaaatgaat ataatctgca gaaaatgcgg tggaatatgg gatatgtgct
     3361 gcagcaaatc gctttgttcc cgaccatgac agtcaagcaa aatattgaag tcatccctga
     3421 aatgttaggt tgggaaaagc agaaaagagc agaccgtgta gatgaacttc ttcaaaaagt
     3481 tggcttatct cctgatattt atcgagatcg tatgcctaga gagttgtctg gaggagaaca
     3541 gcaacggatc gggatcatcc gagctattgc tgcaagccca gatgtgattt tgatggacga
     3601 gccatttagc gcattagatc cgatttcaag aaactcatta caagaattgg ttttgtcctt
     3661 acacgaagaa ttgggcacga ccatcgtttt cgttacgcac aatatggaag aagcaatcaa
     3721 gctaggtgat cgtattgcat ttatgaagga tggagaaatt atccaatgtg acacaccaga
     3781 gcagttattg atgaatccta aaaacgacta cgttcggcat ttctttgatg aaccgaaaca
     3841 gacgaaagag tggcgagtag aagatttagt cgtcaatgga tatttcttaa atgaaatacc
     3901 agaaaatgct cgtcaacaag tttgctttga tacgcctatg aaagaagtat attcgcttct
     3961 ttcggtgtat ccaagcattg tgatagtaga aaagcaaaga agtattgggt cgctgacaag
     4021 taaagaaatc tttgcttttt taagtcgaga ggaggaagga aatgaaaaca tatgatgcaa
     4081 tcatcatcgg ttcaggtgtg agcggcttaa gtgctgctta tggattgaaa gaagcaggta
     4141 aaacagtact agtggtcgaa gaagatctat ggggcgggac ttgtcctaac cgtggatgtg
     4201 atcctaaaaa agtcttgtta agtgcagtag aggcacgtaa tcgggtaaaa caattgagtg
     4261 gaaaaggttt taacgagatc ccaactgcca actgggaaga acttcaaaaa ttcaaacgaa
     4321 catttacgga tccagtccca gaaagccgaa aaaaacaatt agcagaagct gaaatcgatc
     4381 atctttcagg aaccgctcgt tttttggatg attcatctat tgaagtcaac gaagaagtct
     4441 tccatgcgga ttacttagtt ttggcaaccg ggcaaagacc tacgatctta ccagtggagg
     4501 gaaaggaata cttgaaaact agtgcagact ttttatcatt gcctgtactg cctaaagaaa
     4561 tcatttttat cggtggtggt tatattgctt ttgagctagc tacgattgcc aatgctgcag
     4621 ggagcaaagt aaccattgtc catcataatc aacggccatt aaaagaattt gaggcgtctt
     4681 tggtagagga agccgtccat caaatggaag catctggcat tcagtttgct tttggtgttg
     4741 aaacccaaaa gatcatttca gaaggtactc gctatcgttt agtcggaaaa gagacggaat
     4801 tagtagcaga tatgattttt tgtgcgactg ggagacagcc gaatacagaa tctttagcac
     4861 ttgagcaagc gaatatcgtc tttgataaac acggaattgc agtcaatgat tatctgcaaa
     4921 cgagcaatcc aaaaattttt gcgtgtggag atattgtttc aaggaaaacc ccaaaattga
     4981 caccagtcgc cacttttgaa ggaaattatg tagctaagcg gataacggat gcaacaagcg
     5041 aacctatcaa atatccgatt atacctacga tcgtctacgc cagtcctaaa cttgctgaag
     5101 taggagttac aaaaagtcac gcctcttcgt cagatcaagt ggtagagatg gatcttacca
     5161 gttggttcac ctatcatcgt gtcaatgaac cagttgctaa ggctgaattg acctttgacc
     5221 agcaaaatta tttgattggt gctgcagtga ttagcgagca agcagacgaa ttgatcgatg
     5281 atttgacact cgtcatcaat caaaaattga ctaaaaaaga gttggatagc tatatcatgg
     5341 ggtatccgac attggcaagt gatctctcct atttattgaa ataatcagca tgataactaa
     5401 agcgcttgtt gctttttcct tcttgtgcta cactttaaaa aatgaatacc tgtttctggg
     5461 gatgtcgtag acgacatgag agtaagttgt gaacttagac ccattgaacc tgtcagttaa
     5521 tactggtgaa gggagaaaca tagcagacgc ttgaagcggg ttactatgtc gtgcccattt
     5581 caagcgtttt ttgttattca tattagaaga aaggaggtaa caaaatgaag aaaaatcttt
     5641 atccagcagt cagtatggca ttcattttaa ttatttggca gattgttgtc atagtttgga
     5701 aaatacctgc ctttatttta cccggacctg ctgctgtatt ttctgcatta gcggcagatg
     5761 cacaagtatt actttcccat tctgtcacga cattaaatga agcagtgctt ggtttggtaa
     5821 tagctagcgt tctttctttt attactgcct tggcaatgga ttattggaag tttttagaaa
     5881 tgtcttttta tccgttacta gtgatttcac aaatgttgcc aatcatggtg ttagggccat
     5941 tacttaccct atggtttggt ttcggtatgc ttccaaaagt gatccttgtt gtcttgataa
     6001 gctattttcc gatcgtagtt tctttttcgg atacattgag gaaagtatca aaagagcaaa
     6061 tcatcttttt gaaaacgatg ggggcagata cagggaagat ttatcgaata tataaaatac
     6121 cagcgggttt atccggcttc ttttccggtt taaaagtggc tgctacttat tgtgtcagcg
     6181 gagcagtcgt tggagaatgg ctaagcgctc aagcaggctt agggtattac atgatacgcg
     6241 taaaaaacag ctaccagatc gataaagttt ttgctgcgat catttgtgtc attctattga
     6301 gtttattgct gaatggcgct tgttcattat tgaaaaaagg gtattatcgt atgctttata
     6361 ttttttaaga gaaggggatc aaatgaaaaa attatggctt ttattaccgc tactgttgct
     6421 tttatccgct tgtggcactg caaaagaaac aagtagcaaa caagaaataa aggatttgaa
     6481 aaaagtgaca cttgttttgg attatgtacc gaatactaat catactggca tctacctagc
     6541 aaaagaaaaa ggctattata aagaggctgg tttaaatgtc caaatcatcg aaccaggaga
     6601 taattcgaca agtattggtc tagtaggtgc ggacaaagca caatttggtg taagttatca
     6661 agaagatgtc acttatgctc acgcagatgg acagaatata ccagtcaaag caattgctac
     6721 agtgatcaaa cataatactt ctggattcgc caccctatca gacagtaaca tccattcacc
     6781 aaaagatttt gaaggaaaaa catatgcagg ttggcaaagt ccaagtgaag aagctgtctt
     6841 aaaagctgtg atggaaaaag acggcgggga cttttcaaaa ctgacaatgg taggaagcaa
     6901 tggagaaggg ccagaaagct taggaaaatc ctcagatatc caatggtact ttgaaggttg
     6961 ggatatgatc aaagcaaaag aagcgggtat tgaactaaac tatatcccat tgaaagaatt
     7021 agatgaacgg ttagattact atactccggt aattattacg aatgatcaac tgatcaaaag
     7081 tgatccagaa ttggtacagt cttttatgga tgccacgaaa aaaggctatc aggaagctat
     7141 taaggatcca aatgacagtg ctaaactgtt gcaaaaatat gcaaaagaaa atgatcggac
     7201 gtttttagaa gaatcacaag cattcttgtc aaaaaattat acggatgatc ctgagaattg
     7261 gggattgatg gaagaaaaag tttggcgcaa ttatacagct tttatgcagg aaaacggatt
     7321 gatcaagcaa gatgtacctt cgcaagaact attcactaat cagtttataa aataggagtg
     7381 atagatatgt caatccaaat cagccacttg aatgtagtca ttgaaaacct gaaaatatta
     7441 gaagacatcc atttcactat ccctaaagat acctttacat cgattgtcgc gccaagcgga
     7501 gccggtaagt caacattgct gaaaacttta acaggagtcc agcctatgac ttctggaacg
     7561 atcaaagtag acgatcaaaa agtaactggt ctccatacag catttagtta tatgccgcaa
     7621 gaagatatgt tacttccttg gctgagcgtc tatcaaaacg ttacgttata tcaaaaaatc
     7681 aatcatctaa caatcaatga agaacaagta agggattatc tggatatctt tgggttaaac
     7741 ggctatgaac attttttgcc ggaacaatta tctggcggga tgaagcagcg aacggccttg
     7801 ttacgaacaa tcatgaatcc ttcttcttac ttgcttcttg atgaaccatt tggagcgtta
     7861 gatgctatga ccagagggca gatgcaagac tggatgttga agctgcctaa aaaagcaaag
     7921 cggactactt tacttgtgac gcatgatatc gaagaagcaa tttatttatc agacaggatt
     7981 cttgtgcttt ccgcccgtcc ggcacatgtg attgctgaga ttcaagtgcc tgaaaaacga
     8041 cggtcacgtg aatggctact gcagcaatca gagttgaagc aaacgattta tcagttattg
     8101 gcaggtgaat tagatgttaa gtgatgctca ttgtcattta gatgggagtc agagtttagc
     8161 actccttcag caggaacaca cgattttgac gatcatcaat tgtgattcgc cagaagaatg
     8221 gaaggaaaac agacagcttg cagcaagtaa gactcaagcc ttaagttacg gtatccatcc
     8281 ttggaaagca gatagctata cgtttgaaca agttgagcct tttttgaaaa aagcaaggat
     8341 catcggtgaa atagggttag ataatatttg gacgaatgta ccgatgacta cacaaaaaaa
     8401 agtatttgag cgacagcttg cttttgcagc cataaatgag aaacctgttg tactgcacac
     8461 aaaaggatgc gaaaaagaga tcttaaatta tatccaagct taccctaatc actatttgat
     8521 ccattggtat tcttctttgg actatcaaca agactatatt gatttaggct gttatttcag
     8581 cattggtctt gatttgaaaa agaatccagc agtctggcaa ctagctaaag cagtcccaat
     8641 cgaccactta cttattgaaa cggatggact acatgcagca gagtgggctt taggggggat
     8701 tttttcagaa aaagattatg tctcactgct tgaagaacag ttaaagacaa tcgctgagat
     8761 caaagagcta tcagttgaag aagttaaaaa aaatacttat caaaacttgg aacgtttcat
     8821 tagattaggt aaataaaaaa gagatgtgac acaactagtg tcgcacctct ttttggcttt
     8881 ctttgtataa aagtaaactg tttgattaat aaatgaatct tagtaccaac cgtttgcttc
     8941 ccagaatgct ttcgccgctt cccaagaacc ataacgtgaa gtaacatatt gttctgctac
     9001 tctttcttgg tttgcagcag agtagtcacc atttaagtaa gaagaatcta attggtaacg
     9061 tccgatgtaa cgtccgttcg ttgctgtgta agaaccgctt gattcttttt gtgcgatcca
     9121 ttcttttgca gagcttgtgc ttgctggagt agcagcagtt tctgttacag caggagcagg
     9181 ttgtgtttca gcaacaggcg ctgcttgttt ttccgtttga accgtttctt gtacaacagg
     9241 ttgttcagct tggactggtg cttcttgaac ggctgcttga ggttcagcag ctgcttgtgc
     9301 agagccttct gttgggattg ttaattgttg tcctgagta
//
LOCUS       DARBET010000091         9327 bp    DNA     linear   BCT 07-NOV-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4027
            SAMEA114569841-rid20112183.denovo.093, whole genome shotgun
            sequence.
ACCESSION   DARBET010000091 DARBET010000000
VERSION     DARBET010000091.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA114569841
            Sequence Read Archive: ERR12203666
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 9327)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9327)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001972618.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001972618.1
            Genome Coverage       :: 132x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/07/2023 13:18:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,041
            CDSs (total)                      :: 2,975
            Genes (coding)                    :: 2,908
            CDSs (with protein)               :: 2,908
            Genes (RNA)                       :: 66
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 67
            CDSs (without protein)            :: 67
            Pseudo Genes (ambiguous residues) :: 0 of 67
            Pseudo Genes (frameshifted)       :: 26 of 67
            Pseudo Genes (incomplete)         :: 28 of 67
            Pseudo Genes (internal stop)      :: 20 of 67
            Pseudo Genes (multiple problems)  :: 6 of 67
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9327
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA114569841-rid20112183.denovo.093"
                     /isolate="VRE4027"
                     /db_xref="taxon:1352"
                     /country="Denmark"
     gene            complement(<1..363)
                     /locus_tag="SCJ86_002473"
     CDS             complement(<1..363)
                     /locus_tag="SCJ86_002473"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347565.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin family protein"
                     /protein_id="HEG1169988.1"
                     /translation="MRNKKVIVRILIVSFLLFGCFKLIKTNEESNGKLINSNLEEVLE
                     LQKTNQSFFVYLARNDCKKCQEVESFLERYDKKFSRNVYRIETRKEPRQEELQTFLQR
                     YHVKSVPSFLVIHDRNVKK"
     gene            complement(353..2527)
                     /locus_tag="SCJ86_002474"
     CDS             complement(353..2527)
                     /locus_tag="SCJ86_002474"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347564.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF853 family protein"
                     /protein_id="HEG1169989.1"
                     /translation="MMNAPKRPTHFEQMFSVDRKRKQEKETPKSRFQQLYFIVTLLGV
                     CLYPFFIVGSIPLAIVFMIDKKETRAHIYDFEYESFLKRRSSLFFIFALGFTLLNLFC
                     FIFVLPRGYLSCYLLFPLNLFSHALQFNGQTIFALLLGGTGAGSCLIAYASFIEKRKV
                     VSKKERQTALLNSKEYKERRKNKFQESQKYTAEYQQAYQVAIVIKDVALRNKQLASLA
                     KVILLGTDEYGLPYVMNFSELNQHVIIPATTGSGKTTVIQLLAEHACKFRIPVILVDG
                     KGAGETLQALKEVAEAYNRPIEVFVDGGDLRYNPVKNGNDIAIRDKLTNLAETESVYY
                     STAAKALLQVTIQLLDEFQKSHKIERNLVWLQHYLLPRNVLHLFADRIQLKNPDLFKE
                     KVVVEKKKTTKKKTKKNEAMDKTERDFADVEQLSEERIVEHDRKTVDLETYYLLLKEN
                     ISLMTEIEQTLFKRLFVRYEHKKNPFYLYATSENLQTNINMLLDSELGELFDTSGGKR
                     ELDIQEVVKEGKIVYISLNGLIYSEFIRTLAQMLVGDINFFASEVYKQAGETANRSKV
                     LVIFDEPASYLNDNLIDLVNKGRGAGVHAAYAPQTLADIDRIDPVLRKQLIGNVNTFF
                     IGKTNDPTEIDYWADLMGTEEDIELTEMTTQEAGYSDMGKVDWTGERGTKRDVDRFKF
                     NPNRIRALRTGEFVVYRTAEDVQEPPRVVYIRKPNLEKQYAK"
     gene            complement(2543..4885)
                     /locus_tag="SCJ86_002475"
     CDS             complement(2543..4885)
                     /locus_tag="SCJ86_002475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347563.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEG1169990.1"
                     /translation="MRKKSLKWWTLLLLGGLFFCLQGATVSADQMNDGAGVTETDRSE
                     GENANDKVEVKEVTTDVDQLYKNYKKNSFELMTNDYESMNLKKKLSYNVAGVMKNFVW
                     SGVKMLGEFNASMVEFLFELDIISAIREPIQNLTQKIADNMLSIAGTIGISFSAIMMA
                     IKYAGEARMKQALRVFLMTILIFTGLSVLKDSGTNKNLFDVLFSLDSTVETAFVKVNP
                     VLTPEEETNYETKETEEVTADKKVETAGKLMAARVFYSNVYEPYLLLNYGTTNVDTIR
                     EKKVKYAGKEYDRINLLLDNDLGTKIGEKIHDKVTEYEADTLKNKNIQYYNSWKNTAF
                     ALFYFVVNLIQTVVYFVLSMFRLIIGTMQLLLVPMLPFLLFLGLFLVTMNVFGNYAKV
                     FGILTFLKGMTGFACILFSSFLSYGFQLSSTQDDPWEKILLIIVYLFAPLGIYLFRGF
                     LGSLFTGRMGVRGALAFAGNPFGAEAKLRREAKQRLKERKEARKAQKQKEKENARKRQ
                     ERQRKRGGGSDVRMPQTAKQATRSAFRRESAQQPRHKAPNRMERMAQKARDLHDQAKQ
                     AEEQSQERMQRTAMEKQARKTADLLQRELQGNPSLRERSRKGASRSPYRNQGSAMVVS
                     LANPKQKHKSYGQKHTPRTELNDRKRRQGQTSRRRKEALTPVTKTSSMKTRTVAGQKH
                     AVQKPLRKGNVPRATGSTTPKSVRRPEGVREHMSAVNRVTAQTKAPSTVSKAYSTMKQ
                     TKSPKQAPKTRSEFRATQAKMKRKREKQPIYRREMKQRKR"
     gene            complement(4907..7450)
                     /locus_tag="SCJ86_002476"
     CDS             complement(4907..7450)
                     /locus_tag="SCJ86_002476"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347683.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HEG1169991.1"
                     /translation="MAFLFLLVVFIIGIWQFKKKKTKLPRLEFPIDAIIRNFVLTKND
                     DVWVGYQLSSQVFPLNDIDFFKNYLQDGEGILGHDEYDYHFINIPRQFNLDTHIEETI
                     RDLVKGECADLGKVYFEQAGKILKDEVQLNEYRTYLFVRFTTEMKVSDPIEYFQLLKH
                     VFKKFIYRITGQRVPISSVLPTYETLEKELYTELSNYKPIERLSAQEIGQIYYYFFHR
                     SEEKLPERQLTPMEICEGVISNEAGYIAIEQLNQTHYSTFLSQTDAPLAMYGSSFIQN
                     LQDSLSFPIDTHIRLRFEHSKSDERKINKMAGRIYEQEKEYETVDGILDSDDVLNFGT
                     ERLMDVKDKLKRKEKRLCYSTVTLVVSASSKEELEERVKDIEFAIESTDFKFYRSVAD
                     QLTLFNQCLIGSTQRFRSYEQVATTGFVADYGLDLKKEVGNKKGFPLGRIVIAKKFKS
                     AKEALALSSKLVWYYPNLTKKSIEGAEHTNGNTLIIGPPGKGKSVLVKYIFLWLMFLG
                     QKILYIDPKNETQKFFLKALEKFGHIPEFVAMYERINFISLSSDESCRGMLDPLLFLP
                     KEEAIQTAKNVLGMLGEVDTNRETASHKKTVIQDAIDVVYTMPGKHSLSQVIEEIRKS
                     DKELAKLILGHSVGLGKILIGNEHSTPIRFDSQINVLGTQGIRIPTQKEIDSGRLNSE
                     QLAGMAIMEVIMKFTYIFSTNKEEDAAIIYDEAKGYEDTAQGSYLIDDSLRKGRANNT
                     DIYLVVQAFKDYDQEDKKELISYKFAFRPKQKEAREKLLSFFDMEVNSANLEMMEQLQ
                     TGTCLFQDHLGRNQPIAIDVLFDSWLLAVSSTENQDEKIQAMLENEKQR"
     gene            complement(7460..7858)
                     /locus_tag="SCJ86_002477"
     CDS             complement(7460..7858)
                     /locus_tag="SCJ86_002477"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347561.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein"
                     /protein_id="HEG1169992.1"
                     /translation="MEYNYTKEFKHPHKIYGIKGQKIPWISNGIRMEQLVIGGVFLFI
                     LLILFLIAYVNQISFLQTLFRKSWLIMIFGIGVLVWTLFSMKWDNKHFFEYLIGRTSY
                     YQKRKQRFEHEMYVPFFHEKVTYSLHKWKE"
     gene            complement(7939..8223)
                     /locus_tag="SCJ86_002478"
     CDS             complement(7939..8223)
                     /locus_tag="SCJ86_002478"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347560.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEG1169993.1"
                     /translation="MKLFDLKSLFDDVSGQVIWLLAIVAIVLMVGAYATQGFARAAFA
                     IIGVFILIMLVLLLSNAVDIGEWLKNIIFIKDSEENVGVTQMIVPWIQLS"
     gene            complement(8247..9245)
                     /locus_tag="SCJ86_002479"
     CDS             complement(8247..9245)
                     /locus_tag="SCJ86_002479"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein"
                     /protein_id="HEG1169994.1"
                     /translation="MSNAKFNPQAFQTKPPKQQKEKKQKKRFLFQKKKTEPSDRMIQF
                     SQKSAMSVFSGAFFCLFLLMGVVLLLNFGRVDTLTRLAVKKQVNPETLLTEVNQTLSS
                     TEQLKYEGQHFIERLFTYSPEKRNEWEASVNHYLAQGLEASDLGFVTTATNRVAKDVR
                     FIKLEEIDLTQAMYRLFYEVTFTEGEHWKSSQLSFPVSYQNNVLKVLDGVQFLNLSES
                     KNTNSVNYQENRFYSSGQAVTESEEIKLEAFVKRFFELYVKNDEKLALISNEKGLEGG
                     ELINVQLDHAVKNKEGNYFLQGKYRFYFEKGNEFQSNFSMEVKTSGDSYFVTTLNE"
BASE COUNT         2651 a         1999 c         1461 g         3216 t
ORIGIN      
        1 ttttttgacg tttctatcat gaatcacgag aaaacttggg acacttttaa catggtaacg
       61 ttgtaaaaaa gtttgaagtt cttcttgtct tggttctttt cttgtttcaa ttcgatatac
      121 attcctagag aattttttgt cgtatctttc caagaaagat tcaacttctt ggcatttttt
      181 acaatcattt cttgctagat acacaaaaaa tgattgattc gttttttgaa gttctagtac
      241 ttcttctaaa ttactattga ttagttttcc atttgattct tcgttagttt ttatcagttt
      301 aaaacaacca aataagagga aactaacaat aagtatccta acaataactt tcttatttcg
      361 catattgttt ctccaaattt ggtttccgaa tatagacgac tcgcggtggt tcctggacgt
      421 cttcagctgt tcgatacacc acaaattcgc ctgttctcaa tgctcgaatg cgattcggat
      481 tgaatttaaa gcggtctacg tctcgtttcg tgcctctttc tcctgtccaa tcaacctttc
      541 ccatatcaga ataacctgct tcttgtgtcg tcatttccgt taattcaatg tcttcttctg
      601 tccccattaa atctgcccaa taatcaattt ctgtcgggtc atttgtcttc ccaataaaga
      661 acgtattcac attcccaatc aactgttttc gaaggacagg atcaattcta tcaatgtccg
      721 caagcgtttg aggggcatac gccgcatgca ccccagcgcc tcgtccctta ttgactaagt
      781 ctataaggtt atcatttaga taagaagctg gttcgtcaaa tattactagg actttcgagc
      841 gattggctgt ttctcctgct tgtttataca cttctgacgc aaagaaatta atatctccca
      901 caagcatttg agccaatgtc cgaataaact cactataaat caatccgttt aacgagatat
      961 aaacaatctt tccttcttta acaacttctt gaatatctaa ttctcttttt cctccagaag
     1021 tatcaaagag ttcaccaagc tcactatcca ataacatatt gatattggtc tgtaaattct
     1081 cagaagtcgc atacaaataa aacgggttct tcttatgttc gtaacgaaca aacaaccgtt
     1141 taaacaacgt ttgctcgatt tctgtcatga gagaaatgtt ttcttttaat aatagataat
     1201 acgtttctaa gtcaacggtt ttacgatcat gttcaacgat tcgctcttca cttaactgtt
     1261 ccacatcggc aaaatcacgt tctgttttgt ccatcgcttc attctttttc gtttttttct
     1321 tcgtggtttt cttcttttct acgaccactt tttctttaaa taaatctgga tttttcaact
     1381 gaatacgatc cgcaaacaaa tgtaagacgt ttcgtggcaa taaataatgt tgtaaccaaa
     1441 ctagatttct ttcaatttta tggctctttt ggaactcgtc taaaagctga atcgttacct
     1501 gtaataacgc tttggctgct gtcgaataat acacgctttc tgtttctgct agattggtta
     1561 atttatctcg aatggcaata tcattgccat tttttacagg attataacgc aaatcgccac
     1621 cgtccacaaa gacttcaatc gggcgattgt atgcctctgc cacttctttt aacgcttgta
     1681 aagtctctcc tgctcctttc ccatcgacta agataacggg gatacgaaac ttacacgcat
     1741 gttctgctag taattggatc accgtcgttt ttcctgaacc tgtagtcgct ggaataatca
     1801 catgttggtt caattcacta aagttcatca cataagggag cccatactca tctgttccta
     1861 atagaatcac tttggctaat gaagccagtt gtttattccg caaagcaacg tcttttatga
     1921 caattgctac ctggtacgct tgctgatact ctgctgtata tttttgggac tcctgaaatt
     1981 tattcttccg acgttccttg tattctttac tatttagcaa agctgtttgc cgttctttct
     2041 ttgaaacaac ttttcttttt tcaataaaag acgcatacgc aatcaaacaa cttcctgcac
     2101 ctgtcccacc taaaaggagg gcaaaaatcg tttgtccatt aaactgcaac gcatgagaaa
     2161 acaagtttaa tggaaataat aagtaacacg acaaataccc tctaggaaga acaaaaataa
     2221 aacaaaatag atttaatagc gtaaaaccta acgcaaaaat aaaaaaaaga gagcttcttc
     2281 gttttaagaa gctctcatac tcaaagtcat aaatatgtgc acgtgtctct tttttatcta
     2341 tcataaagac aatcgctaaa ggaatactgc ctacaataaa aaatggatat agacaaacac
     2401 ctaataaagt gacgataaaa taaagttgtt gaaaccgact ttttggcgtt tctttttctt
     2461 gttttctttt gcgatcgaca gaaaacattt gctcaaaatg tgtcggtcgt tttggtgcat
     2521 tcatcatact atcgatttcc tcctatcgtt ttcgttgttt catttctctt cgataaatcg
     2581 gttgtttttc tcgttttcgt ttcatttttg cttgagttgc tcgaaactca ctccgtgtct
     2641 tcggtgcttg ttttggtgat tttgtttgtt tcatggttga gtatgcttta gatacggttg
     2701 aaggcgcttt ggtttgtgct gttactcgat tgactgctga catatgttcc cgaaccccct
     2761 ctggacgtcg aacagatttc ggtgtggtag aacctgttgc tcgtggtaca ttcccttttc
     2821 ttagtggttt ttgtaccgca tgtttttgtc ctgcaactgt ccgggttttc atcgaacttg
     2881 tttttgttac gggagtaagt gcctccttcc gacgacgact tgtttgtccc tgacgacgtt
     2941 ttcgatcgtt caattctgtt ctaggcgtat gcttctggcc ataagacttg tgtttttgct
     3001 ttggattcgc taatgacacc accattgcag aaccctgatt gcgatacgga ctacgagacg
     3061 caccttttct acttctttct cttagactag gattgccttg caattctcgt tgtaataaat
     3121 cggctgtttt tcttgcctgt ttctccattg ctgttcgttg catacgttct tgactttgct
     3181 cttctgcttg tttcgcttga tcatgcaagt ctcttgcttt ttgtgccata cgttccatac
     3241 gatttggtgc tttatgacga ggttgttgtg cggattcacg ccgaaaagca cttcgtgtgg
     3301 cttgtttcgc cgtttgtggc atacgaacgt ctgagccacc cccacgtttt cgttgacgtt
     3361 cttgtctctt ccgtgcgttt tctttttctt tttgtttctg tgcttttctc gcttctttgc
     3421 gttctttcaa ccgttgtttg gcttcccttc tcaattttgc ttctgcccca aatggatttc
     3481 ccgcaaaagc taacgctcct cgaacaccca ttcttccagt aaatagactg cctaaaaagc
     3541 ctcggaacaa ataaattccc aacggcgcaa acaaataaac aatgatcaag agaatttttt
     3601 cccatggatc gtcttgtgta ctacttaatt ggaacccgta actaagaaaa gaagaaaaaa
     3661 ggatacatgc aaaacctgtc attcctttta aaaacgtcaa aataccaaaa actttggcat
     3721 agttaccaaa cacattcatt gtgacaagaa acaatcctaa aaaaagtaag aacggcaaca
     3781 tcggtactaa caatagttgc atagttccaa taatcaatcg aaacatactt aagacaaaat
     3841 aaacaacggt ttgaatcaga ttcacgacaa aataaaacaa ggcaaatgct gtatttttcc
     3901 aactattgta atattgtatg ttcttgtttt tcagtgtatc tgcttcatac tctgtgactt
     3961 tgtcatgaat tttctcccct atttttgtac ctaaatcatt gtccaataaa agattgattc
     4021 gatcatattc ttttccagca tatttgactt tcttctcacg aattgtgtct acattggttg
     4081 tgccataatt taacaacaga tatggttcat aaacattact ataaaagacc cgagccgcca
     4141 tcaatttgcc tgccgtttcc acttttttat ccgctgttac ctcttctgtt tcttttgttt
     4201 cataatttgt ttcttcttca ggtgttaaca ctggattgac cttcacgaac gccgtttcca
     4261 ctgtagaatc taaagaaaaa agaacatcaa aaagattctt atttgtccca gaatctttta
     4321 atacgcttaa cccagtaaaa ataaggatcg tcataagaaa gacacggagt gcttgtttca
     4381 ttcgagcctc tccagcatat ttgattgcca tcataattgc cgaaaaactg attccaatag
     4441 ttcctgcaat acttaacata ttgtccgcaa ttttttgtgt taagttttga atgggttctc
     4501 gaatggcgga aataatatct aattcaaaga gaaattcaac cattgaagca ttgaattctc
     4561 ctaacatttt tacgccactc caaacaaaat ttttcatcac tcctgcaaca ttgtacgata
     4621 gctttttttt aaggttcatt gactcatagt cattggtcat caattcaaac gaattctttt
     4681 tgtaattttt atacaattga tccacatcag tcgttacttc ttttacttct actttatcgt
     4741 tggcattctc tccttcagaa cgatcggttt cagtgacacc cgcaccatca ttcatttgat
     4801 cggcagaaac agtagcccct tgtagacaga aaaaaagacc ccctaataaa aggagcgtcc
     4861 accatttcag agattttttt cgcaaaaaaa ccacctcttt tttgttttat cgttgttttt
     4921 cattttctaa cattgcttga attttttcat cttgattttc agtagaagaa acagccaata
     4981 accaactatc aaacaacaca tcaatggcaa tcggttgatt tcttcccaaa tgatcctgga
     5041 ataaacaggt tcctgtttgt aattgttcca tcatttctaa attggctgaa ttcacttcca
     5101 tatcaaagaa ggacaatagt ttctctcgtg cttctttttg tttcggacga aacgcaaatt
     5161 tgtaagaaat taactctttt ttatcttctt gatcgtaatc tttaaacgct tgcacaacaa
     5221 ggtaaatatc cgtattattc gcacgtcctt ttcgcaagct atcatcaatc aaatagcttc
     5281 cttgtgcggt atcttcatac ccttttgcct cgtcataaat aatcgcagcg tcttcttctt
     5341 tgtttgtaga aaagatatac gtaaacttca taatgacttc cataattgcc attcctgcaa
     5401 gttgttcgct atttaatcga cccgaatcaa tctctttttg tgtcggaatg cgtattcctt
     5461 gtgtccctaa cacgttgatt tgtgaatcaa aacgtatcgg cgtactgtgc tcattcccaa
     5521 tgagaatctt tcctaaacca acgctatgcc ctaaaatcaa tttagctagt tctttgtcac
     5581 tttttcgaat ttcttcgata acttgactta acgaatgctt acctggcatg gtgtaaacta
     5641 catcgatggc gtcttggatc accgttttct tgtggctcgc tgtttctcga ttggtatcta
     5701 cttctcctaa cattcctaac acatttttag cggtttgaat tgcctcttct tttggtaaaa
     5761 acaatagagg atcaagcatc cctcgacagc tttcatctga agataacgaa ataaaattaa
     5821 ttcgttcata catagccaca aactctggta tgtgtccgaa tttttcaagt gcctttaaaa
     5881 agaacttttg ggtttcattt ttcggatcaa tataaagaat tttttgacct aaaaacatca
     5941 gccataaaaa aatatatttt acaaggaccg attttccttt acctggtgga ccaataatca
     6001 acgtatttcc attggtatgt tccgcgcctt caatactttt ctttgttaaa ttgggataat
     6061 accatactag tttactagat agcgctaacg cttctttcgc agatttaaac ttttttgcaa
     6121 taacaatccg tcctaaagga aatcctttct tatttcctac ttctttcttt aagtccaagc
     6181 cataatctgc cacaaaacct gtcgtagcga cctgctcgta actacgaaaa cgttgcgtac
     6241 ttccaattaa gcattgatta aacaacgtta gctgatccgc taccgatcga taaaatttga
     6301 aatctgtaga ctcaatcgca aattcaatat ctttgactcg ttcttccaat tcttcttttg
     6361 aagaagcaga tacaaccagc gtgacggtag aataacacaa tcgtttttct ttgcgcttca
     6421 gcttatcttt tacatccatt aaccgttctg taccaaaatt taatacatcg tctgaatcta
     6481 aaatgccgtc aacggtctca tattcttttt cttgttcata aatccgtcca gccattttat
     6541 taatttttcg ttcatcagat tttgaatgct caaatcgaag tcgaatatgc gtatcaattg
     6601 gaaaagataa gctatcctga agattttgaa taaagctact cccatacatg gctaaaggag
     6661 cgtccgtttg acttaaaaac gtcgaataat gcgtttgatt caactgttca atcgcaatgt
     6721 atcctgcttc attcgaaatg acgccctcgc aaatttccat tggcgtaagc tgtctttcag
     6781 gcaatttttc ttctgatcga tggaaaaaat aataataaat ttgtccaatc tcttgggcag
     6841 ataagcgctc tatcggctta taattggaca attccgtata gagctccttt tctaatgttt
     6901 catacgttgg taacacacta cttataggaa ctcgttgtcc cgtaatacga tagataaact
     6961 ttttaaaaac atgcttaagc aattgaaaat attcgatcgg atcagacact ttcatttctg
     7021 tagtgaaacg cacaaacaga tacgttcgat actcgtttaa ttgcacttcg tcttttaaaa
     7081 tctttcctgc ctgttcaaag tacactttcc caagatctgc gcattctccc tttaccaaat
     7141 ctcgaatggt ttcttcaatg tgcgtatcta ggttaaattg acgtggaata ttgataaaat
     7201 gatagtcata ttcatcatgt ccaagtatgc cttcgccatc ttgaagatag ttcttgaaaa
     7261 aatcaatgtc atttaaagga aatacttggg aagaaagttg atagcctacc cacacatcat
     7321 catttttggt caaaacaaaa tttcggataa tggcgtctat cggaaattct aatcgaggaa
     7381 gcttcgtttt tttctttttg aactgccaaa tcccgataat aaagacgacc aaaagaaaca
     7441 aaaacgccat ttactcgact cactccttcc atttgtgtag ggaatacgtc actttttcat
     7501 ggaaaaacgg cacatacatt tcatgttcaa agcgttgttt ccgtttctga taataggacg
     7561 ttcgcccaat taaatactcg aaaaagtgtt tattgtccca tttcattgaa aacaatgtcc
     7621 aaacgagaac accaatccca aaaatcataa tcaaccagga ctttcgaaac agtgtctgca
     7681 agaaactaat ttgattcaca tacgcaatca aaaataggat caaaagaatg aagagaaata
     7741 ccccacctat gactaattgt tccatgcgaa tgccattaga aatccaggga attttttgtc
     7801 ctttaatccc gtaaattttg tgagggtgct taaactcctt tgtatagttg tattccatgt
     7861 tcactctacc tccttttaca aaaaaagaag cgaccagaag ttctaatctt ctgatcgctt
     7921 ttgcagtttg tctttttctt atgataattg tatccagggc acaatcattt gcgttacccc
     7981 cacgttctct tcactatcct taataaagat aatgttcttt agccattcac caatatcaac
     8041 ggcattcgat aacaataaga cgagcattat caaaataaag acgccaataa tggcaaacgc
     8101 tgcccgcgca aagccttggg tcgcataagc accaaccatt agaacaatcg caacgattgc
     8161 caatagccaa atgacttgtc cagatacatc atcaaataac gatttaagat caaaaagttt
     8221 catttccatt ctcctctaag ttcattttat tcatttaacg tagtaacaaa ataactatcg
     8281 ccacttgttt ttacttccat tgaaaagttt gattggaact cgtttccttt ctcaaaatag
     8341 aaacgatact tcccttgcaa aaagtagttt ccttctttat tttttactgc atgatccagt
     8401 tgtacattga tcaattcgcc gccttctaac cctttttcgt tagaaatcaa tgccaatttt
     8461 tcatcatttt tgacatataa ctcaaaaaac cgtttcacaa atgcttccag cttgatttct
     8521 tcgctttcag tgaccgcttg cccagaagaa taaaatcgat tttcctgata gttcacagaa
     8581 ttggtattct tactctcaga aagattcaaa aattgaacgc catcaagtac ttttaaaaca
     8641 ttgttttgat aactgactgg aaaagaaagt tgactgcttt tccaatgttc tccttctgta
     8701 aaagtaacct cataaaatag gcgatacatt gcttgtgtca aatcgatttc ttctagttta
     8761 ataaagcgaa catctttcgc tactcgattg gtagccgtcg ttacaaagcc tagatcagac
     8821 gcttccaatc cttgtgctaa ataatgattg acggaagcct cccattcgtt tcgtttttct
     8881 ggcgaataag taaataacct ttcaataaaa tgttgtccct cgtacttcaa ttgttccgta
     8941 gaagacaagg tttggttgac ttcagttaac aatgtttctg gattgacttg ttttttcact
     9001 gctaaacgcg ttaatgtatc cactcgtcca aagtttagga ggagcacgac acccattaac
     9061 agaaacaaac aaaaaaaagc gcctgaaaat acactcatgg cacttttttg cgaaaattgg
     9121 atcattcgat ctgacggttc tgttttcttt ttttggaaca aaaaacgttt cttttgtttc
     9181 ttttccttct gttgtttcgg tggcttagtt tggaacgctt gtggattaaa ttttgcatta
     9241 ctcatgagaa atgccatacc gttctaacaa taatttcggt attttgtcca caatcactac
     9301 ttcgtggaca accacacttg ccccctc
//
LOCUS       DARBET010000092         9281 bp    DNA     linear   BCT 07-NOV-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4027
            SAMEA114569841-rid20112183.denovo.094, whole genome shotgun
            sequence.
ACCESSION   DARBET010000092 DARBET010000000
VERSION     DARBET010000092.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA114569841
            Sequence Read Archive: ERR12203666
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 9281)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9281)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001972618.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001972618.1
            Genome Coverage       :: 132x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/07/2023 13:18:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,041
            CDSs (total)                      :: 2,975
            Genes (coding)                    :: 2,908
            CDSs (with protein)               :: 2,908
            Genes (RNA)                       :: 66
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 67
            CDSs (without protein)            :: 67
            Pseudo Genes (ambiguous residues) :: 0 of 67
            Pseudo Genes (frameshifted)       :: 26 of 67
            Pseudo Genes (incomplete)         :: 28 of 67
            Pseudo Genes (internal stop)      :: 20 of 67
            Pseudo Genes (multiple problems)  :: 6 of 67
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9281
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA114569841-rid20112183.denovo.094"
                     /isolate="VRE4027"
                     /db_xref="taxon:1352"
                     /country="Denmark"
     gene            <1..435
                     /locus_tag="SCJ86_002480"
     CDS             <1..435
                     /locus_tag="SCJ86_002480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377182.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase M23"
                     /protein_id="HEG1169995.1"
                     /translation="YSGQQLTIPTEGSAQAAAEPQAAVQEAPVQAEPVQAEQPVVQET
                     VQTETQAAPVAETQPAPAVTETAATPASTSSAKEWIAQKESSGSYTATNGRYIGRYQL
                     DSSYLNGDYSAANQERVAEQYVTSRYGSWEAAKAFWEANGWY"
     gene            complement(759..842)
                     /locus_tag="SCJ86_002481"
     tRNA            complement(759..842)
                     /locus_tag="SCJ86_002481"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(806..808),aa:Leu,seq:cag)
     gene            1370..1870
                     /locus_tag="SCJ86_002482"
     CDS             1370..1870
                     /locus_tag="SCJ86_002482"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335673.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEG1169996.1"
                     /translation="MKLIKLWEADLKKAYQLQTSFGPNESGFINNAYGYTFAEFLDYV
                     KKCEKTSLGIDLPESYVPATVFILESDEHEYVGIFNLRHKLNETLRNGAGHIGYGISP
                     NYRKKGYATQGLALVLQEAKKIGIDEALLSVNKKNLGSLKAQQKNGAAIYREDEKEYY
                     TKIKLM"
     gene            complement(2060..2404)
                     /locus_tag="SCJ86_002483"
     CDS             complement(2060..2404)
                     /locus_tag="SCJ86_002483"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875052.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal-sulfur cluster assembly factor"
                     /protein_id="HEG1169997.1"
                     /translation="MSEANQERSGQEIELIKERILAALEMVIDPELGIDIVNLGLIYD
                     IEFNPENGETVIKMTLTTMGCPLADILTESIHDALKEVPEVTKAEVKLVWYPAWTTDK
                     MSRYARIALGIR"
     gene            complement(2558..3478)
                     /locus_tag="SCJ86_002484"
     CDS             complement(2558..3478)
                     /locus_tag="SCJ86_002484"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350761.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YitT family protein"
                     /protein_id="HEG1169998.1"
                     /translation="MTAYHKNERLKKIVVILITGLTAAVALNYFLIPANVFSAGMNGI
                     AQIISSLLYEWFHIKIDTGTFIFLLNIPVFVLGFLKVGKRATILSFVNVVCVSVMTML
                     LPVGQITDNILMNALVGGVLLGLGVGLSLKMGFTTGGMDIISLVLSQTTGKTVGNYML
                     ILNGIIVVIAGFFFNWESALYTIISIYAMTQVIDAIHTSHQKVTAMIVTVKPEEVTEE
                     LAKRMVRGMTLLPSVGGYSKKQGNMIMMVITRYEMYDLDQIVHEIDDDAFINILPTHS
                     VFGRFANENEQKMYRTTGVLPEIKNRSQKK"
     gene            3665..4156
                     /locus_tag="SCJ86_002485"
     CDS             3665..4156
                     /locus_tag="SCJ86_002485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347660.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YajQ family cyclic di-GMP-binding protein"
                     /protein_id="HEG1169999.1"
                     /translation="MAKDASFDIVSEVNTEEVKNAIQQTTKELSNRFDFKDSTVEIKL
                     ENSNLVVLSDDDFKIEQIKDVLFGKLIKRNVPIKNIHFSESEHALGGKARQTADLVNG
                     IDRENAKKITTAIKNEKLKVKAQIQEDQIRVTGKNRDDLQAVIALLRKLDLPIELQFT
                     NYR"
     gene            complement(4180..6555)
                     /locus_tag="SCJ86_002486"
     CDS             complement(4180..6555)
                     /locus_tag="SCJ86_002486"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327943.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent DNA helicase"
                     /protein_id="HEG1170000.1"
                     /translation="MIKQSKISVRHLVEFILRRGSIDNRKKSNHTALEGARIHRKLQK
                     DAGENYEKEVFLKTTVQLDEYYLIVEGRADGIFKRDGIYYIDEIKTSEPRFEDLEPEQ
                     VELFFHQARVYAYIYSQEHDLQEINLQLTYFQTAEEVITRKVEHQTREQLETFFKKLT
                     EDYKEWLVFQENWRTVRNASLMALKFPHDEYRKGQRELAVAAYKTIRTKQKLFVEAPT
                     GTGKTISTLFPALKAVGEEEGEKIFYLTAKTITRQVAEDAMTALKDTGAEVKSVTLTA
                     KDKICFLDETTCNPDQCPYANGYYNRINEGLWDLLNHENQITREVIETYARKHTLCPF
                     ELSLDVSIWCDVIIGDYNYLFDPTVYLRRFFEDEKNEDYLFLIDEAHNLVNRSREMYS
                     AELSYEKTKRSKEAIPKEFKKLHRRFNKLLKEFDSIREIAKEDHWDYHHQKAPAESLV
                     KAGYQLSEKIKEWLAEFPEHPSQEQLLPYYFDLLHFLKVSEFYDDHYETTVEKTYRDL
                     IVKEFCIDPSLFLEQSLDKGKSSILFSASFSPLSYYQETLGGQKSLAYKLPSPFPEGN
                     QQVFIANYLETTYRKREHSLAKLVETIHAFADGKVGNYFVFFPSYQYLDLAVEAFRQQ
                     YPDSNVLIQETDMNEEERERFLTKFQTNPAETLIGFCVLGGIFSEGIDLRGDRLIGSM
                     IVGVGLPQMNHEQELIKSYYDEKEHLGFAYAYQLPGMNKVLQAGGRVIRGMADQGIVV
                     LADQRFSSFSYRRLFPQHWQTAREVRSVGELEEGIQRFWLSKEAVNQKNEE"
     gene            6881..7507
                     /locus_tag="SCJ86_002487"
     CDS             6881..7507
                     /locus_tag="SCJ86_002487"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340072.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HEG1170001.1"
                     /translation="MHIERLKTGMIEENCYLVYNEEALLIIDPGEDAERIKQEIKKTN
                     QQPVAILLTHTHYDHIGAVEPLRAYYQIPVYVSPLEQKWLGDPILNLSGLGRHDDIAD
                     VIVSPAEYEFEMKTYTLGNMSFRVVPTPGHSIGSVSFIFDDFVVSGDALFRGSIGRTD
                     LYTGNLEQLLHSIRTQLFVLPDEFAVYPGHGDATTIEQEKRTNPFFNR"
     gene            7530..8234
                     /locus_tag="SCJ86_002488"
     CDS             7530..8234
                     /locus_tag="SCJ86_002488"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348910.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine phosphatase family protein"
                     /protein_id="HEG1170002.1"
                     /translation="MTETTLYIVRHGKTMFNTIERVQGWCDTPLTKQGQEGIHYLGRG
                     LKNVDFSFAYSSDSGRAIETARIILGEHSKGKEIPYFIEKRIREWCFGSLEGGYDMEM
                     WGVIPRVLNFKTYDEMFTTEVSFEQIANAVYNLDTAGWAETYETLRDRVWTGFEDIAH
                     HCEKNGGGNVLVVSHGLTIAFLLNLIDPKQPVRAGLLNGSVTKVTYANGRFSIHGIND
                     TSYIDNGKTHSRDSFL"
     gene            8426..9259
                     /locus_tag="SCJ86_002489"
     CDS             8426..9259
                     /locus_tag="SCJ86_002489"
                     /EC_number="3.6.1.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="undecaprenyl-diphosphate phosphatase"
                     /protein_id="HEG1170003.1"
                     /translation="MFFANIIKAVILGIIEGITEWLPISSTGHLILADEFIKLDASSQ
                     FMSMFNVVIQLGAILAVVVLYFHKLNPFSPSKSTVEKKDTWTLWFKVIVACIPAIIIG
                     LPLDDWLEANFHKFLPVAIVLIVYGIAFIIVEKRNKNKTPRWTSLNDFTYQAALLVGL
                     FQVLALVPGTSRSGATILGSILIGASRFVATEFSFFLGIPVMFGASGLKIVKYLAEGN
                     SFKMEETVVLLVGTFVSFVVSVLAIKFLLSYLKKNDFTAFGWYRIILGIILIGYWFIA
                     M"
BASE COUNT         2828 a         1791 c         1651 g         3011 t
ORIGIN      
        1 tactcaggac aacaattaac aatcccaaca gaaggctctg cacaagcagc tgctgaacct
       61 caagcagccg ttcaagaagc accagtccaa gctgaacctg ttcaagctga acaacctgtt
      121 gtacaagaaa cggttcaaac ggaaacacaa gcagcgcctg ttgctgaaac acaacctgct
      181 cctgctgtaa cagaaactgc tgctactcca gcaagtacaa gctctgcaaa agaatggatc
      241 gcacaaaaag aatcaagcgg ttcttacaca gcaacgaacg gacgttacat cggccgttac
      301 caattagatt cttcttactt aaatggtgac tactctgctg caaaccaaga aagagtagca
      361 gaacaatatg ttacttcacg ttatggttct tgggaagcgg cgaaagcatt ctgggaagca
      421 aacggttggt actaagattc attatttgaa cgtttaaaca cattcaaaat atagtaagag
      481 gtcatgcgat ttattctttc gcatgacctc tttttgagtt taagtttagt ctttattcct
      541 taactatcgt ttgccagtat ttcttgtatc agaatctata aatctactat ttgaatcaat
      601 ctattaggtt ttaaactgtc ccaccttcac acaaattttt tttgatttat ttgaatctgg
      661 ttccgttgtt taaaattttt cctatattct tttagataat atgttatttt ctttctcatc
      721 gctccaggtg taccttctct ttataaaaaa acaaattatg cggctaagaa gactcgaact
      781 tccacgaggt caccctcact agcgcctgaa gctagcgcgt ctgccaattc cgccatagcc
      841 gcatcacatc ttttagttta ggattacatg tgttttttgt caagaacata tttttgttct
      901 ttttcatttc tgtacgccct cccttatgcc atctacacaa ataattcatc cgagcaactc
      961 catcatctat tgagggttgg tgaaaaaatc tgttttatcc atatgatcga tttcaatgtt
     1021 aaacgaacga ctctgggatt agtaactatt ttattttttt cttcatttct tttttcctct
     1081 ttttctcgat tcagctatct tctttcattt ggatattgat tatttcttct gttgattact
     1141 atccttctgg aattctagac gctgaagtaa ttcacgtttt ctttcttcta gagatcataa
     1201 gtaacgaatg aacgaacttc aaataatttt tttattgtaa ttcatatttg tattgttcga
     1261 tgattcttaa tacgatatga cacaataaat aaaaataggc atggctattt acaacaatag
     1321 tgctatagta gaaaacaaat gaataaggtt acttaaagag gtgaatacga tgaaactgat
     1381 caaactatgg gaagcagatt taaagaaagc ttatcagcta caaactagct ttggaccaaa
     1441 tgagagtgga tttatcaaca atgcttatgg ctatacattt gctgaatttt tagattatgt
     1501 aaaaaaatgc gaaaaaacgt ctttaggaat cgatcttcct gagagttacg ttcccgcaac
     1561 cgtctttatt ttagaaagtg atgaacatga gtatgtagga atttttaatt tgcgccataa
     1621 actgaacgaa acattaagaa acggcgctgg ccatattggt tacggcatct cccctaatta
     1681 tcgtaaaaaa ggatatgcca cacaaggatt agcattagta ctacaagagg caaaaaaaat
     1741 cggcattgat gaggcacttt tgtctgtaaa taaaaaaaat cttggcagtc ttaaagcaca
     1801 acaaaaaaat ggggctgcga tttatcgtga agatgagaaa gaatactata cgaaaatcaa
     1861 actgatgtaa aaaagaagga ttccaaaagg aaagggattt gaatgtcctt tatcaataga
     1921 acacatttat ttaattaact tataatatag cagacaaaat tttataaaca aagaaacctt
     1981 ttatctcttt tcttctagaa aggataaaag gcttttttgt ttttcctatt tgcttctcat
     2041 agctagtcag ccaagatttc tatcgtatcc ccaacgcaat ccgtgcataa cggctcatct
     2101 tatctgtcgt ccatgctgga taccagacta gtttgacttc tgctttcgtt acttctggca
     2161 cttcttttaa tgcgtcatgg atagattctg ttaaaatatc tgctaatgga cagcccatag
     2221 ttgttagagt catcttaatg actgtttctc cattttcagg attgaattct atatcataaa
     2281 tcaagcctaa attgacgatg tcgattccta attcaggatc aatcaccatt tccaaggcag
     2341 caagaatacg ttctttgatt agttctattt cttgtccaga ccgttcttgg tttgcttcgc
     2401 tcatcgtacg acactcctta acacgatttt tatttatcat actctttttt tagctgcttg
     2461 taaatgagaa tttagaagta agattctcgt ttaaatttat aaaaatgttc ttattagatg
     2521 taatagatta aatttattct aaaataaacc tgattcctca ttttttttgt gaacgatttt
     2581 ttatttctgg caatacgcca gttgttcgat acattttttg ttcgttttca tttgcaaacc
     2641 ggccaaatac tgaatgagtt ggtaaaatat tgataaatgc atcgtcgtcg atttcatgaa
     2701 cgatctgatc aagatcatac atttcgtagc gggtgatgac catcatgatc atgttccctt
     2761 gtttttttga atagccaccg acagacggca gtaatgtcat tccacgtacc atccgttttg
     2821 ctagttcttc tgttacctct tctggcttga cggtaacgat cattgctgtt actttttgat
     2881 gactcgtgtg gatggcatca attacttgag tcattgcata aatggaaata atcgtataca
     2941 aggcactttc ccagttaaag aagaagcctg cgatgactac aatgatacca tttagaatca
     3001 gcatgtagtt tccgactgtt tttccagttg tttgtgaaag tacaagagag atgatatcca
     3061 tcccaccagt tgtaaagccc atttttaaag ataatcctac gccaagacct aataaaacac
     3121 cgcctactag ggcattcatc aaaatattat cagtgatctg tccgactgga agcagcatcg
     3181 tcatcaccga tacacataca acattcacaa aactaaggat cgttgctcgt tttcctactt
     3241 tgagaaaccc taatacaaac acagggatat tcaaaagaaa aataaatgtt cctgtgtcta
     3301 tcttgatatg aaaccattca tataaaagcg aggaaatgat ttgagcgatc ccattcatcc
     3361 ctgcagaaaa gacattggct ggaatcaaaa aataatttaa agcgaccgca gcggtcaatc
     3421 cggttatcaa aataaccact attttcttta atctttcatt tttatgatac gcagtcataa
     3481 ttttactcac aagcacctct cccctacttt ggtattccaa atgtaagaat aacagtttat
     3541 ctattaaaat aaaatacttc gaactcaaat gagaagaatt ttagcaaaca ggtaagaatc
     3601 tgccctaatc ctatgataag atagatgtat ggtaaatatt tatatgtata tcaggagggt
     3661 tcctatggca aaagatgcta gcttcgacat tgtatcagaa gtcaatacag aagaagtaaa
     3721 aaatgcgatt cagcaaacaa caaaagaatt gtccaatcgc tttgacttca aggattctac
     3781 agtagaaatc aaattagaga acagcaatct agttgtattg agcgatgatg atttcaaaat
     3841 cgaacagatc aaagatgtat tatttggtaa gctgatcaaa agaaatgtgc caatcaaaaa
     3901 tatccatttt tccgaatcag aacacgctct aggtggcaaa gctcgacaaa ctgccgattt
     3961 ggtcaacgga atcgatcgtg aaaacgcgaa gaaaatcact acagctatca aaaatgaaaa
     4021 actgaaagta aaagcacaaa tccaagaaga tcagattaga gtgacaggta agaaccgtga
     4081 tgatctacaa gctgtgattg ctttattacg taaacttgac ttaccaatcg agttgcaatt
     4141 cactaactat cgttaatcaa aaaacgaggc tgacgtctgt cattcctcgt ttttttgatt
     4201 tactgcttct tttgataacc aaaaacgctg tattccttct tccaattccc caacacttcg
     4261 tacttctctt gctgtttgcc aatgttgtgg gaataaacgt cgataagaaa aactagaaaa
     4321 tcgttgatct gccaatacaa cgattccttg atccgccatg ccacgaatca ctcgaccacc
     4381 agcttgcaag actttgttca ttccaggaag ctgataagcg taagcaaatc caaggtgttc
     4441 cttctcatca taatatgatt tgatcagttc ctgctcatga ttcatctgcg gcaaacctac
     4501 acctacgatc atactgccga tcaatcggtc ccctctcaaa tcgattcctt ctgaaaaaat
     4561 cccgccaagt acgcaaaatc caatcaatgt ttcagcggga tttgtttgaa attttgttaa
     4621 aaatcgttcg cgttcttcct cattcatgtc cgtttcttgg attagcacat tgctgtcggg
     4681 atattgctgt ctgaatgctt ccaccgcaag atctaaatat tgataagaag gaaagaaaac
     4741 gaaataattc ccgacctttc catcagcaaa agcatgaatc gtctctacta attttgccag
     4801 actgtgttcc ctttttcgat aagtcgtctc taggtaattt gcgatgaaaa cttgctggtt
     4861 tccttctggg aaaggactcg gcagtttgta agccagactt ttttgtcccc ccagtgtttc
     4921 ctgataataa gagagtggtg aaaaactagc agaaaaaaga atactgcttt ttcctttatc
     4981 caacgactgc tcaagaaaca agcttggatc aatacaaaac tcttttacta tcagatcgcg
     5041 atacgttttt tcgactgttg tttcgtagtg atcatcataa aattcactga ccttcaaaaa
     5101 atgaagcaaa tcaaaataat aaggaagcaa ttgttcttgt gatgggtgct caggaaattc
     5161 tgccagccat tctttaattt tttcgcttaa ttgataacct gcctttacta aagattcagc
     5221 cggtgctttt tgatgatgat aatcccaatg atcttctttt gctatttctc ggatcgaatc
     5281 aaattctttt aatagtttgt tgaaacggcg atgaagtttt ttgaattcct ttggtatagc
     5341 ttccttactt cgtttcgttt tttcataaga tagttctgcc gaatacatct ctcgggaacg
     5401 attcaccaaa ttatgtgctt cgtcaatcaa aaacagatag tcctcgtttt tttcatcttc
     5461 aaaaaaacgt cttaaataaa cagttggatc aaataaataa ttgtaatccc caatgattac
     5521 gtcacaccag atactgacat ctaatgatag ttcgaaagga caaagtgtat gctttcttgc
     5581 ataagtttcg atgacttcac gagtgatctg attctcatga ttcaaaagat cccataaacc
     5641 ttcattaatc cgattgtaat aaccattggc atatgggcac tgatctggat tacaggtcgt
     5701 ctcatctaaa aagcagatct tgtctttcgc cgtcaaagtg acacttttta cttcagctcc
     5761 tgtatctttt aatgctgtca tagcatcttc agctacttgt ctagttattg ttttagccgt
     5821 tagataaaag atcttttctc cctcttcttc ccctacagct ttcaaagcgg ggaataacgt
     5881 agagattgtt tttcctgttc cggtaggtgc ttctacaaac aatttctgtt tcgtacggat
     5941 cgttttataa gcagctaccg ctagttcacg ctgacctttc ctatactcat catgggggaa
     6001 tttcaaagcc atcaaagagg cattgcgtac agtgcgccag ttttcttgaa aaacaagcca
     6061 ttctttataa tcttcagtca gctttttaaa aaaagtttct aactgctctc ttgtctgatg
     6121 ttcaaccttt ctcgtgatca cttcttctgc cgtctggaag taagtcaatt gcagatttat
     6181 ttcttgtaaa tcatgttctt gactatagat ataggcatac acccttgctt gatgaaagaa
     6241 aagttccact tgttctggtt ctaaatcttc aaatcgtggt tcggaggttt tgatttcatc
     6301 tatataataa ataccgtctc ttttgaaaat accgtctgca cggccttcaa cgatcaggta
     6361 atattcatct aattgaactg ttgtttttaa aaagacttct ttttcatagt tttctcccgc
     6421 atctttttgg agcttgcggt ggattctcgc tccttctaat gcagtatggt tacttttttt
     6481 acgattgtcg atacttccac gtcgcaaaat aaattctact aagtgccgca cagatatttt
     6541 tgactgtttg atcatttcca ccacctcttt ctctctaaga ttacctgaaa aaacctagtt
     6601 tagcaaaaag agctctcttt ccaaacataa aaaaacaccc tgctgattag gcagggatat
     6661 aggatataga atttggattt ttggatgaat ctaatgtaac aaggacatat taaaatgcta
     6721 tgaaaactta ctttccttct ctttagaata tccaaatact tttcatatag tctgataatt
     6781 cagataagta aagagaaata agtacctgtt tttgtttcct agcaatgaat gcgttatact
     6841 aatggatgaa agaagttttg gctatcaagg aggaatgcac atgcatatcg aaagattaaa
     6901 aacaggaatg attgaagaaa actgttattt ggtctacaac gaagaagcac ttttgatcat
     6961 tgaccctgga gaagatgcgg aacgaatcaa acaagaaatc aaaaaaacaa accaacagcc
     7021 ggtagcgatt ttgcttaccc atactcacta cgatcatatc ggtgcagtgg aacctttacg
     7081 cgcgtattat caaatcccag tttatgtcag cccactagag caaaaatggc taggtgatcc
     7141 gattttgaat ctttctggac tcggtagaca tgatgatatt gctgatgtta ttgtctcgcc
     7201 tgcagaatat gaatttgaaa tgaaaactta tactttagga aacatgtctt ttcgcgtcgt
     7261 ccctacccca gggcattcta taggaagcgt cagctttatt tttgatgatt ttgtcgtttc
     7321 tggcgacgcc ctgtttagag gaagtatcgg tagaaccgat ctgtatacag ggaatttaga
     7381 acaattactt catagtattc ggacccagct gtttgtatta cctgatgaat ttgcagtcta
     7441 tccaggacat ggcgatgcaa cgacgattga acaggaaaaa aggacaaatc cttttttcaa
     7501 tcgttaaaac gacttaggag gagaataaaa tgacagaaac tacactttat atcgtacgac
     7561 acggaaaaac gatgttcaat acaattgaac gagtacaagg ctggtgtgat acacccctca
     7621 caaagcaagg acaagaaggc attcattatt taggtcgcgg gttaaaaaat gtcgactttt
     7681 catttgctta ttctagtgac agtggtcgtg caatcgaaac tgcccgtatt attctaggag
     7741 aacattctaa gggaaaagaa attccttatt ttattgaaaa aaggattaga gaatggtgtt
     7801 tcgggtctct tgaaggtgga tatgacatgg aaatgtgggg tgtgatccct cgtgtattga
     7861 attttaaaac atatgatgaa atgtttacca cagaagtttc ttttgaacaa attgctaatg
     7921 ctgtatacaa cttagatacc gctgggtggg cggaaactta tgaaacattg cgagaccgtg
     7981 tatggacagg atttgaagat atcgcccatc actgcgaaaa aaatggtggt ggaaatgttt
     8041 tagtcgtatc tcatggtttg acgatcgcct ttttgcttaa tctgatcgat cctaaacagc
     8101 ctgttcgtgc tgggctttta aatggcagcg taacaaaggt cacttatgca aacggtcgat
     8161 tttctatcca cggcatcaat gatacgtctt atatcgataa cgggaaaaca catagtcgcg
     8221 actcgtttct ataatgggga tattttaatt tttaccagag aatcataaaa ggcaaagtct
     8281 gaacgagggc tttgtcttct tttttctctt cgtgtactta tctgctttat ttctcaagtt
     8341 tttcttaata ttaaaaaaca gattgggttt ctcaaaaaat aacggtatac tataagatgt
     8401 atcttaattg ggaaggaagg aatttatgtt ttttgcaaat attatcaaag cagtgattct
     8461 cggtattatt gaaggaataa cggaatggct gcccatcagt agtactggtc atttgatcct
     8521 agcagatgaa ttcatcaaac tggatgccag ttcacaattt atgtcgatgt tcaatgtcgt
     8581 tatccaatta ggggcgatct tggctgtcgt cgttctttat tttcacaagt taaacccatt
     8641 ttctccaagt aaatcaacgg tagaaaaaaa agatacgtgg acactttggt ttaaagtgat
     8701 cgttgcttgc ataccagcca tcatcattgg tttgcctttg gatgattggt tagaagcaaa
     8761 tttccacaag ttcttaccag ttgccattgt tttgatcgtt tacggtattg cctttattat
     8821 tgtcgaaaaa cgcaacaaaa acaaaacacc tcgttggact tctctgaatg actttacgta
     8881 ccaagcagct cttcttgttg ggttattcca agtcttggca cttgttcccg gaacatcccg
     8941 ttctggcgca acgatcctag gatctatctt gattggtgct tcgcgttttg tcgctacaga
     9001 gttttcattt ttcttaggga ttcctgtcat gtttggcgca agcggcttga agatcgtcaa
     9061 atatttagct gaagggaatt cattcaaaat ggaagaaact gttgttcttc ttgtaggaac
     9121 attcgtatct tttgtggttt ccgtcttagc tatcaaattc ttactaagct atttgaagaa
     9181 aaacgacttt accgcttttg gctggtatcg tattatcctt ggtatcattt tgattggtta
     9241 ttggtttatt gcaatgtaga aaaaaccaga acacttctct t
//
LOCUS       DARBET010000093         8904 bp    DNA     linear   BCT 07-NOV-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4027
            SAMEA114569841-rid20112183.denovo.095, whole genome shotgun
            sequence.
ACCESSION   DARBET010000093 DARBET010000000
VERSION     DARBET010000093.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA114569841
            Sequence Read Archive: ERR12203666
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 8904)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8904)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001972618.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001972618.1
            Genome Coverage       :: 132x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/07/2023 13:18:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,041
            CDSs (total)                      :: 2,975
            Genes (coding)                    :: 2,908
            CDSs (with protein)               :: 2,908
            Genes (RNA)                       :: 66
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 67
            CDSs (without protein)            :: 67
            Pseudo Genes (ambiguous residues) :: 0 of 67
            Pseudo Genes (frameshifted)       :: 26 of 67
            Pseudo Genes (incomplete)         :: 28 of 67
            Pseudo Genes (internal stop)      :: 20 of 67
            Pseudo Genes (multiple problems)  :: 6 of 67
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8904
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA114569841-rid20112183.denovo.095"
                     /isolate="VRE4027"
                     /db_xref="taxon:1352"
                     /country="Denmark"
     gene            complement(305..661)
                     /locus_tag="SCJ86_002490"
     CDS             complement(305..661)
                     /locus_tag="SCJ86_002490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344588.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arsenate reductase"
                     /protein_id="HEG1170004.1"
                     /translation="MEVKVYGSAHCVTSLKAKQWLEHHQLPYQFVDLEERDLSTEEAE
                     ELCSFEEVQAEQLFATWSEAFHNIAIDLSCVGKKQLIFLCTQHTNLLRRPLIIIDEVL
                     FIGYNEQLMEQLILNK"
     gene            complement(675..1085)
                     /gene="spx"
                     /locus_tag="SCJ86_002491"
     CDS             complement(675..1085)
                     /gene="spx"
                     /locus_tag="SCJ86_002491"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator Spx"
                     /protein_id="HEG1170005.1"
                     /translation="MIKVYTSPSCTSCRKARAWLQANQLEFEEKNIFAEPLTESEIKK
                     ILAATEGGVQDIISTRSKIYEKLGIDFNELTLKQMIVLFKEYPSLLRRPILIDEKRIL
                     IGFNEDEIRSFVPREIRCVARNLIYLQNNNILNS"
     gene            complement(1432..3618)
                     /locus_tag="SCJ86_002492"
     CDS             complement(1432..3618)
                     /locus_tag="SCJ86_002492"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent helicase"
                     /protein_id="HEG1170006.1"
                     /translation="MNEKEILRLQKELKRWENKYKKLQQVFRLQEEIIATYEAAYGEA
                     PGLIEEEVQVKESEGNSSFFKMMEKRSYNRFNQEQKAAITYDMKKHLRIIAGAGSGKT
                     QTICAKAVYLMTQKQVDEERILMITFTRNAANELKKRVDNFSQRKTAVHIGTFHSIFF
                     RIYNEICRKFPEVAMQGIQGDFSKDTAQKVNAVLQQLIRKYNLYIFDQYGEKTIASRL
                     DYWQNMNLSVEEMVQLVKEKYDSIDKNARQPISERLYGLLTELQEQKRSQQLLEFNDV
                     LQNLKSALENEEIQHYIGQKYDYLFIDEFQDTNPLQWEIVKRMTKENRIKLIVVGDDD
                     QSIYGFRGSEPAYIKNFEKEYPTKTLFLLTNYRSRAAIVQGANRLISYNKNDRIPKAM
                     IPAQKEAGVMEAYLFSDTKAESQWLIKQIQTFIQKNGKYKESIILYRSASQTQQLVQS
                     LLKTNIPFVLEADSPYEGIFGIKSFQHFYQKIVHWQTALNLQKKNQAYQQLLRQLMAD
                     CYLKKSECDQYFSPKYQNIAVADYILSISPNLKSKSAEIREFEEALRKTDQHQEIYSL
                     VQSYLHLPRIAKEIDQSDQEWIREEVKQHATFRRLQALSQETQATAKELKTRLIAYRQ
                     GKLDALCIQSIHKSKGLSYRNVFLIGCNEGSLPYNGATEKKVIDFNEIKAEPVTTIEE
                     ERRLFYVAMTRARNRLYLCVPQMKNSRKLKVSRFIKETGSRVKKGK"
     gene            3836..4519
                     /locus_tag="SCJ86_002493"
     CDS             3836..4519
                     /locus_tag="SCJ86_002493"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286265.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1275 domain-containing protein"
                     /protein_id="HEG1170007.1"
                     /translation="MQTSFHEGRTIGLLLTFIGGAMDSYTYIQYGAFASAQTGNIILS
                     IIQAFDGEWLSVGKKVLSTLFFFLGILLTKYLIDYFRKKEKHFWRLFVLYYEAVIFFF
                     VSLTPVHEHPALVTILIAFTASIQWVAFDKINGRAYTNLFTTGNLKGVATNLYDYCIS
                     KEKADFDRFFHYLSVVLAFIAGAIVLVFCHHLFGAHSILIVSILFLIMALTQTFHLWQ
                     FYHTNRFTI"
     gene            complement(4600..5319)
                     /locus_tag="SCJ86_002494"
     CDS             complement(4600..5319)
                     /locus_tag="SCJ86_002494"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853096.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="deoxynucleoside kinase"
                     /protein_id="HEG1170008.1"
                     /translation="MPIIVIIVAGTIGAGKSTLTEMLAQDLETKPFYENVEDNEVLPL
                     FYSNPEKYTFLLQIFFLNKRFLAIKDAFSHDDNVLDRSIYEDSMLFHLNADLGRVSEV
                     EVKQYEGLLETMLKELEEISPQKKPDLLVYIRVSFETMLARIKKRGREYEQLEQDPEL
                     YSYYKELNRRYEEWYEQFDICPKIVIDGDKYDFVADPACGEQIVQEIKMRAKKMTEEA
                     DAQSIRYTTNNPKAKGIFTNK"
     gene            complement(5631..5837)
                     /locus_tag="SCJ86_002495"
     CDS             complement(5631..5837)
                     /locus_tag="SCJ86_002495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337349.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEG1170009.1"
                     /translation="MENEQLFEVGETYAFKSDLFESPIKGKIEKIYENSVMIRVMKCA
                     VEDNDTAHNLHWQLVVRKSSTIEE"
     gene            6082..6684
                     /locus_tag="SCJ86_002496"
     CDS             6082..6684
                     /locus_tag="SCJ86_002496"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296063.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="accessory gene regulator B family protein"
                     /protein_id="HEG1170010.1"
                     /translation="MKDKVVNKLLEALFSGDKSEEDMVYVQVKFALEVLLNNLGKLAV
                     VLVFSLVTGSWAETGITFLSYICIRRYAYGLHSDSEFVCLLWTLLYLWGVPLVMKHLQ
                     LTISFPMMVFLLISCFLLLLRYGSRGTAINPIEPEKRPPLLKKAISMFLIFSLITLFF
                     SASYFSTYLLLGIVLEIATLLPITNYLMNFGGIFHEKNNH"
     gene            6825..8105
                     /locus_tag="SCJ86_002497"
     CDS             6825..8105
                     /locus_tag="SCJ86_002497"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002316678.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HEG1170011.1"
                     /translation="MFLWSIALQYLIYTICILLIDHHIRYKYLFAGVILISCILIGWV
                     YSILGIFTALIAIGMLMLTFFFFSTSRQQLVYALPFGLLTSLLGDHLTSIDDFFVLKS
                     SVIEPGDSLQYFHIVLSAFLSALLAFCLKKSLETWFHTVDRRMIGTIGTLILFTYYII
                     IFYTRFSGETPDVLALNTSFFILYLCVGLIILVYYWKISNKKLADQLLEQQVRMQQDY
                     IKDLEKNYQDLREFKHDYQNLLFSLHSYISEGDLEGLRSYYDQTILPTRQMMNLFPAN
                     LSMLDNMKVPEIRSLLSLKLMMAQEKGLIVQLHFPEPIELDTKHTVNLVRMLGIILDN
                     AIEGASAAKKKKIELTILKKNHSVMIRVINTTTNTLPLNQLKQKGFSTKTNPKNEGLG
                     LFILDELTKTNPYLFLETSIENQRFSQTIYIQIK"
     gene            8118..8846
                     /locus_tag="SCJ86_002498"
     CDS             8118..8846
                     /locus_tag="SCJ86_002498"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286278.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HEG1170012.1"
                     /translation="MIPIYICEDDPKQLEVLSTVIKNRIMIENLDSYVHISTSDPEEL
                     LNAAKIRTSSFGIYFLDIELEDSEMDGVAIGKLIRELDPLGKIIYVTSHTEMSMKILK
                     SNIEPTDYIVKEDLYDLKENVEHILSKIFEDFQMSQIQKDIFKIEFNDEIKFLPIKDI
                     QYFSTSLGAPHKLEVHLTHAQLQFYEKIKEIEKMHRNFIRCHKSYVINTQNVRSINKK
                     TREVTLANGETIPASIRGLKKLIS"
BASE COUNT         2587 a         1667 c         1463 g         3187 t
ORIGIN      
        1 actcagttat cttatcatgt catttctaac atgtcaacaa ctattttata tgttttttaa
       61 aatactattt tattgctttg ctacttaaaa caacagaaat gaatatatca tgtaaattta
      121 cacatgtcaa tatattttta actctatatc cccacctaga tttgaatttt gaccacataa
      181 attgagcatg tctctatagt agaaaaataa agtcaaaaaa agaacacata acacgcttga
      241 tgctaagtgt tcgactgtta caactgtatg aaatgctcag tgacttgatt aaaacagtta
      301 aagtctattt atttaagata agctgttcca tcaactgctc attgtatcca ataaatagaa
      361 cttcatcgat aataatcaaa ggacgtctga ggagattggt atgttgtgtg cataaaaaaa
      421 tcagttgttt ttttcctaca caggaaaggt caatcgcaat gttgtgaaaa gcctcagacc
      481 aagtcgcaaa tagctgttct gcttgcactt cttcaaaact gcataattct tctgcttctt
      541 ctgtacttag atcgcgttct tctaagtcaa caaattgata aggcaattga tggtgttcta
      601 accattgttt cgccttcaga cttgttacac agtgcgctga tccatagact ttcacttcca
      661 cgaaggctcc ctccctatga atttaagatg ttattgtttt gtaaatagat cagatttctg
      721 gcaacacatc gaatttcacg agggacaaat gaccggatct catcttcatt aaatccgatt
      781 agtattcttt tttcatcaat caaaataggt cgtctcaata atgaaggata ttctttgaat
      841 aagacgatca tttgctttaa agttaattcg ttaaaatcta tgcccagctt ttcgtaaatt
      901 ttcgaccgag tggaaataat atcttgaact cctccttcag tcgctgccaa gatctttttg
      961 atctcacttt ctgttaacgg ttcggcaaaa atattcttct cttcaaattc aagttgattc
     1021 gcttgcaacc atgctcttgc ttttctacag gaagtacagc taggcgaagt atatactttt
     1081 atcattttct cactcatttt taattataat ccaattctac agtaatttct tttaaaatac
     1141 caattattag ctaataatat ataaaaaaaa atcaaaataa ctgattattt acgtttgaat
     1201 aatgagaatt tttctatata ttctctattt tgataatagt attctcattt tcaaaataaa
     1261 taagggaggt gtgacaaaat tcttgtcaca cctccctttt ccgaataaac tgtgttccag
     1321 aaatagcttc ttcgaaaata agctgaaatt tcacaaaaat ttgagaagca attttcgtac
     1381 attccttctt ctttcttgaa actgaccact tctgtcacac cctctcttct atcactttcc
     1441 ttttttcaca cgactcccgg tttctttgat aaatctcgat actttcaatt ttcttgaatt
     1501 tttcatttga ggtacacata gatacagacg atttcttgct ctagtcatcg caacataaaa
     1561 tagtcttcgt tcttcttcta tcgtcgtcac tggttctgct tttatttcat taaagtcgat
     1621 tactttcttt tctgtcgctc cgttataagg caaacttcct tcgttacaac caatcaaaaa
     1681 gacatttcgg taactaagac ctttactttt gtgaatgctc tggatacata gtgcatcaag
     1741 ttttccttgt ctgtaagcaa tcaagcgggt ttttaattct ttagctgtcg cttgggtttc
     1801 ttgcgataag gcttggagtc ttctgaaagt agcatgctgc tttacttctt ctcgtatcca
     1861 ttcttgatca ctctggtcga tttccttagc tatacgtggc agatgaagat agctttgaac
     1921 aagcgaatag atttcttgat gctggtctgt tttacgcaac gcttcctcaa actctcggat
     1981 ttctgcagat ttcgatttta aattggggct tatcgacaaa atataatctg caacggctat
     2041 attttgatat ttaggagaaa agtattgatc gcattcactt tttttcaggt agcagtctgc
     2101 catcagctgc cggagcaatt gctggtaggc ctgatttttc ttttgcagat tcagtgctgt
     2161 ttgccaatga acaatttttt gatagaaatg ttggaaagac ttgataccaa agataccttc
     2221 atatggacta tctgcttcca gtacaaaagg aatattcgtt ttcagcagag attggacaag
     2281 ctgctgcgtt tgtgaagctg atcggtataa tatgattgat tccttatatt tcccattttt
     2341 ttgaataaat gtctgaattt gtttgattag ccactgactt tctgctttag tatcagaaaa
     2401 caaataggct tccataacac ctgcttcttt ttgtgctgga atcatcgcct ttggtatccg
     2461 atcattttta ttatagctga tcaatctatt tgctccttgg acaatagctg cccgagaacg
     2521 ataattagtc aataagaaaa gagttttggt aggatattct ttttcaaagt tcttgatgta
     2581 tgctggctct gatcctctga atccatagat actctgatca tcatctccaa caacaatcaa
     2641 cttgatcctg ttttcctttg tcatccgttt cacgatttcc cattgaagtg gattagtatc
     2701 ttgaaattca tcaatgaata gataatcata tttttggccg atataatgct ggatctcctc
     2761 attctccaaa gcgcttttca gattttggag tacatcattg aactctagca attgctggct
     2821 tcttttttgt tcttggagtt ccgtgagcaa tccatacagt cgttctgata tcggctgtcg
     2881 tgcattctta tcaatggaat catatttttc tttaactaac tgaaccattt cttctactga
     2941 aaggttcata ttctgccaat agtctaaccg agaagcaatc gttttttctc catattgatc
     3001 aaatatataa agattatatt tacgtatcag ctgttgcaaa acagcattca ctttttgtgc
     3061 agtatccttt gaaaagtctc cttgtattcc ttgcattgct acttcaggaa attttcgaca
     3121 aatttcattg tatatcctaa aaaagatact atggaatgtt ccgatatgga cagcggtttt
     3181 tctctggctg aaattatcta ctcgtttttt tagttcattt gcagcgttcc ttgtaaaagt
     3241 gatcattagt atgcgttctt cgtctacttg tttttgcgtc atcagataaa cagcttttgc
     3301 acagattgtt tgcgttttgc cgctcccagc tcctgctatg attcgaagat gtttcttcat
     3361 atcatatgtg atagctgctt tttgttcttg attgaagcga ttataggaac gtttctccat
     3421 cattttaaag aaagagctgt ttccttcact ttcttttact tgtacttctt cctcaataag
     3481 tccgggtgct tcgccataag ccgcttcata ggtagcaatg atttcttctt gtagccgaaa
     3541 gacttgttga agctttttat acttgttctc ccagcgtttc aattcttttt gtaatcggag
     3601 aatttctttt tcattcaact gattgatttc cttcccatct ttcttctttg aatttgttta
     3661 tttcaagttg gttatttaaa gtttcgttgt ctattttttc tttatactaa cagacctttc
     3721 taaaaatgaa aatgggaagc gatatgattt tagttttctg aaaatttttc ctttttttca
     3781 atcgtttatg ctaagataag gaagacaaaa atattcataa aaggtgatga aaaaaatgca
     3841 gacttccttt cacgaggggc gtacaatagg acttttgctg acatttatcg gaggagccat
     3901 ggacagttat acttatatcc aatatggtgc ttttgcctct gctcaaactg gcaatatcat
     3961 tctatcgatc atccaagcat ttgacggaga atggctaagt gttggtaaaa aagtattgtc
     4021 tacccttttc tttttcttag ggattcttct aacaaagtat ctaattgatt attttagaaa
     4081 aaaagaaaaa catttctggc gcttgtttgt tctatattat gaagcagtga tttttttctt
     4141 tgttagtttg acacccgtcc atgagcatcc cgcattagta acgattttga ttgcttttac
     4201 tgcatcgatc cagtgggtag cttttgataa aatcaacggg cgtgcctaca ctaatttatt
     4261 tacaactgga aatttaaaag gggtagcaac gaacttatat gattattgta tttctaaaga
     4321 aaaagcagat tttgatcgat ttttccacta tctaagtgtc gttcttgcct ttatcgcagg
     4381 agctattgtc cttgttttct gtcatcatct gtttggcgcc cattcgattt tgatcgtttc
     4441 cattttattt ttgatcatgg ccctcactca gacatttcat ttatggcaat tttatcatac
     4501 gaatcggttc actatttgat ctcaatgaat caaaaaaact aagcaagatg ctgctcacag
     4561 agaaatatct gcgagtgagc aatttgctta gtttttatat tatttatttg taaagattcc
     4621 ctttgctttc gggttatttg tagtgtagcg gatcgactgg gcatctgcct cttctgtcat
     4681 tttctttgcc cgcatcttga tttcttgtac gatttgttct ccacatgccg gatcggctac
     4741 gaaatcatat ttgtccccat cgatcactat tttgggacat atatcaaact gttcatacca
     4801 ctcttcgtat cgtcgattga gttctttata gtaggaatag agttcaggat cttgttctag
     4861 ctgttcatat tcccggcctc tttttttgat tctcgctaac atagtttcaa aggatacacg
     4921 aatataaacc agtagatctg gtttcttttg tggcgaaatt tcttctaact ccttcagcat
     4981 cgtttctaaa agaccttcat actgctttac ttctacttca gaaacacgcc ctaaatctgc
     5041 attcaaatga aacagcatac tatcttcgta gatggagcgg tctagtacgt tgtcatcatg
     5101 gctgaaagca tctttgattg ccaaaaagcg tttgttcaaa aagaaaatct gtaataaaaa
     5161 agtatacttt tctggattag aataaaatag cggcaatact tcattatctt ccacgttctc
     5221 ataaaaaggt tttgtttcta aatcttgggc aagcatttca gttagtgtgc ttttccctgc
     5281 accgatcgtt cctgcaacaa tgatcacaat tatcggcatc cttcctgtaa taaaatctgt
     5341 tcttcatttt actacttcat ttatagaaat aaaagccttg actttatctg ctccttaaaa
     5401 gctacttctc taaaagtagc caaaagtaac tcctattcat gaaaataaag gctgtgacaa
     5461 aagtcttgtc gcagcccttt tttcaaataa acagtattct cctgccaaca cctcggtcac
     5521 aaggcaatat tcataaaaca tgaaaagctg ttttttaaca ttattccttg taactcagag
     5581 ctaaacggga gactcacaac ctctttatgc tctcaacatt ttatctgttt ttattcttct
     5641 attgtacttg atttgcgtac aactaactgc cagtgcagat tatgagcggt atcattgtct
     5701 tctactgcgc atttcatgac acgtatcatc acactatttt catagatttt ttctattttt
     5761 ccttttattg gtgattcaaa taaatcagat ttaaatgcat atgtttcgcc gacttcaaac
     5821 aattgttcat tttccataag aatccctcca tcacctttta taaggtcagt aattttttaa
     5881 cataaatcac tcgaaaaagc acaaacttgt cataaaaagt acttttcgta tcaaaactgt
     5941 cacttttttt ggtaaggttc ttggaaaacg aagatttttt taggagatca tgacggtttt
     6001 gatgtcaaaa ccacttttta tgaaacaaaa gacgtttttt atttttttta gcataaaatt
     6061 tgtggtggag gtacgtatga gatgaaagat aaagttgtga acaaattatt agaagcctta
     6121 ttcagcggag acaaatcgga agaagatatg gtgtatgtcc aagtaaaatt tgccttagaa
     6181 gttttattga ataatcttgg aaaactagcg gtcgttctag tcttttctct ggtgacaggc
     6241 tcatgggcgg aaacaggaat cacctttctt tcttatatct gcatccgcag atatgcttat
     6301 ggattgcatt ccgactctga attcgtttgt ctgctgtgga cactacttta tttatggggc
     6361 gttcccttgg tgatgaaaca tctgcaattg actatctcct tcccaatgat ggtctttttg
     6421 ttaatcagct gctttttgtt actgcttcgc tatggatcaa gaggtacagc aatcaatccc
     6481 attgaacccg agaaacgtcc cccattatta aagaaggcga tcagcatgtt tctaattttt
     6541 tctctcatta ctttattttt ctcagcttct tacttttcta cctatttgct acttgggatt
     6601 gttttagaaa tagctacgtt gcttcctatt acaaactact tgatgaattt tggaggaatc
     6661 tttcatgaaa aaaacaatca ttaaacaatt aactaactta tctgactttt ttgtatctgg
     6721 ctgctgcttt ggtatgagtt ctgaattcaa aatccctgaa aaattaaaaa aataatacgc
     6781 taattgtgct ataatttggt aaaaatcaat gggagctgta ctttatgttt ttatggtcca
     6841 tagcgttgca atatttgatt tacacaattt gtatcttatt gattgaccat cacatccgat
     6901 acaaatatct gtttgcggga gtcattctga tttcttgtat tttgattgga tgggtctatt
     6961 cgattttagg gatttttact gctctaattg ctataggaat gctgatgctt acttttttct
     7021 tcttttcgac ttcgcgtcaa caattggttt acgcgcttcc ttttgggtta ttgacgtcgc
     7081 ttttaggaga tcacttaaca agcatcgacg atttttttgt tttgaaatct tcggttattg
     7141 aaccaggaga ttcattgcag tattttcaca ttgtcttatc cgctttttta tctgctttat
     7201 tagctttttg tttaaaaaaa tcgttagaga cttggtttca cactgtggac cgccgtatga
     7261 tcggtacgat cgggacattg atattattta catactatat cattattttt tatacccgtt
     7321 tttctggtga aacacctgat gtcttagctt tgaatacgag ttttttcatt ctatatctgt
     7381 gtgttggcct gatcatcttg gtttattatt ggaaaatctc taataagaaa ttagctgatc
     7441 agttattaga gcaacaagtc aggatgcaac aggattatat caaagatcta gaaaaaaact
     7501 atcaagactt gagagaattc aaacatgatt accaaaatct gcttttttct cttcacagct
     7561 atatttcaga aggcgactta gaaggattac ggtcttatta tgatcaaacg atattaccta
     7621 cacgccagat gatgaacctt tttccggcca atctaagtat gttggataat atgaaagttc
     7681 ctgagatccg tagtttactg tcattaaaac tcatgatggc acaggaaaaa gggttgatcg
     7741 tccagcttca ttttcctgag ccaatcgaac tggatacgaa acatacggta aaccttgtac
     7801 gtatgcttgg tattatcttg gacaatgcga ttgaaggagc tagtgctgca aagaagaaaa
     7861 aaatcgaatt gaccatactc aagaagaatc attccgtgat gattcgtgtg atcaatacaa
     7921 caacaaatac attaccgtta aaccagttga aacaaaaagg gttctcaaca aaaacaaatc
     7981 caaaaaacga aggccttggc ttgtttatct tggatgaatt gacaaagacc aatccttatc
     8041 ttttccttga aacatcaatt gaaaaccagc gtttttctca aacgatttat atacaaataa
     8101 aataaaggag taatgatatg attcccattt atatttgtga agacgatcca aaacaattag
     8161 aagttttatc aacggtgatc aaaaatagaa tcatgattga aaacttagac agttatgtcc
     8221 atatttctac ctctgatccg gaagaacttc tcaatgcagc caagatcaga acttcttctt
     8281 ttggtattta ctttttagat atcgaactag aagacagtga aatggatggt gtagcgatcg
     8341 gtaaattgat ccgtgaatta gatcctttag gaaaaataat ctatgtgact tcccatacgg
     8401 aaatgtcgat gaaaatctta aaaagcaaca tcgaaccgac tgattatatc gtcaaagaag
     8461 atctttatga tttaaaagaa aatgtcgagc atatcctgag caaaattttc gaagattttc
     8521 aaatgtccca aatccaaaaa gatatcttca aaatagagtt taatgatgag atcaaatttt
     8581 taccgatcaa agacatccaa tatttttcta cttcgctagg agcgccacat aaactggaag
     8641 tccatttgac tcatgcccaa ttacaattct atgagaagat caaagaaatc gagaaaatgc
     8701 atcggaattt tatccgttgt cataaatcct atgttatcaa tacacaaaat gtgcggtcga
     8761 ttaataaaaa aaccagagaa gtcacgttgg caaacggcga gaccatccca gcctctatcc
     8821 gtggattaaa gaaattgatt tcataaaaag aaccgaatag tttggaccga ctaaatgacc
     8881 agccgatcca aacccttcgg ttct
//
LOCUS       DARBET010000094         8800 bp    DNA     linear   BCT 07-NOV-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4027
            SAMEA114569841-rid20112183.denovo.096, whole genome shotgun
            sequence.
ACCESSION   DARBET010000094 DARBET010000000
VERSION     DARBET010000094.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA114569841
            Sequence Read Archive: ERR12203666
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 8800)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8800)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001972618.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001972618.1
            Genome Coverage       :: 132x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/07/2023 13:18:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,041
            CDSs (total)                      :: 2,975
            Genes (coding)                    :: 2,908
            CDSs (with protein)               :: 2,908
            Genes (RNA)                       :: 66
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 67
            CDSs (without protein)            :: 67
            Pseudo Genes (ambiguous residues) :: 0 of 67
            Pseudo Genes (frameshifted)       :: 26 of 67
            Pseudo Genes (incomplete)         :: 28 of 67
            Pseudo Genes (internal stop)      :: 20 of 67
            Pseudo Genes (multiple problems)  :: 6 of 67
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8800
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA114569841-rid20112183.denovo.096"
                     /isolate="VRE4027"
                     /db_xref="taxon:1352"
                     /country="Denmark"
     gene            <1..778
                     /gene="menA"
                     /locus_tag="SCJ86_002499"
     CDS             <1..778
                     /gene="menA"
                     /locus_tag="SCJ86_002499"
                     /EC_number="2.5.1.74"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="1,4-dihydroxy-2-naphthoate
                     polyprenyltransferase"
                     /protein_id="HEG1170013.1"
                     /translation="NYMDYEKAHSEVYKYEENVIGRRNLSPVLIRNMILAMIAFVFVI
                     GVILTINTGWLLLLMGFICCFIGVFYTFGPIPLSRMPLGEIFSGVTMGLGIFAMVVYV
                     NTFGSDVYDLTVNFSSGQFDLVGNLWAIAAIVLASLPLVFTIANIMLANNLRDLDRDI
                     ENHRYTLVFYIGRPAGTVLFQLLMYACYFVILMGLLSGIYEWPILVTFLTLPIVKKHL
                     DQFKQTLPQPISFSYSIKNMVLFNASYVLGLLLTIIMQAI"
     gene            complement(1592..2938)
                     /gene="gor"
                     /locus_tag="SCJ86_002500"
     CDS             complement(1592..2938)
                     /gene="gor"
                     /locus_tag="SCJ86_002500"
                     /EC_number="1.8.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376012.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione-disulfide reductase"
                     /protein_id="HEG1170014.1"
                     /translation="MQIYDYIVIGGGSGGIASANRAGMHGAKVLLIEANELGGTCVNV
                     GCVPKKVMWQASTILETIERDASSYGIQADLKNVDFAGLVERREKYIDFLHGAYQRGL
                     DSNHVEAIKGYATFIDSQTIEVNGETYRAPKILIATGGRASKMTIPGGEYAIDSNGFF
                     ELTELPKRAIVLGAGYIAAELSGVFRGLGSEVMWAYRKERPLRTFDKMLSDNLIQMYQ
                     EAGIKTYAHHIAKEITKNNDEYTVLFENGETLTGDCVLFAGGRVPNTEKLGLENTNVE
                     LDKKGFIKVDKYQNTTDEHIFAVGDVIGKLDLTPVAIAAGRRLSERLFNGKKDSYLDY
                     KLVPTVVFTHPPIATIGLTEEEALEKYGENELKVYRSRFTPMYFALNDYRQKCEMKLI
                     CVGKEERIVGLHAIGVGVDEMLQGFAVAIKMGATKEDFDNTVAIHPTGAEEFVTMR"
     gene            complement(3072..4325)
                     /locus_tag="SCJ86_002501"
     CDS             complement(3072..4325)
                     /locus_tag="SCJ86_002501"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304804.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="U32 family peptidase"
                     /protein_id="HEG1170015.1"
                     /translation="MMKEMTVKRKLKRPEILAPAGTLEKLKTAIHYGADAVYIGGNAY
                     GLRSRAGNFTYEEMAEGVAFAKEHDAKVYVAANMVTHEGNEDGAGEFFRSLRDVGISA
                     VIVSDPALIEICATEAPGLPIHLSTQASATNFETLEFWKEEGLERVVLAREVSMEEVA
                     EIRKNTDTEIEAFIHGAMCISYSGRCTLSNHMSMRDANRGGCSQSCRWKYGLFDMPFG
                     QERNSLTDTGEVEEEFSMSAVDMSMIEHIPELIENGVDSFKIEGRMKSIHYVSTVSNV
                     YKAAVDSYMEDPENYVCKQEWIDELWKVAQRELATGFYYNTPTENEQLFGERRKIPVY
                     KFVGEVLSYDEATQIATIRQRNLFSVGDEIEFYGPGFSHFSQTVEVMHNEEGESIDRA
                     PNPMMILTMPVAQPVKPGDMIRKRK"
     gene            complement(4331..5254)
                     /locus_tag="SCJ86_002502"
     CDS             complement(4331..5254)
                     /locus_tag="SCJ86_002502"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016922287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="U32 family peptidase"
                     /protein_id="HEG1170016.1"
                     /translation="MIELITTVETIEQAEQLLAAGADTLYFGEEQFGLRLPASFTREE
                     QRQLVELAHKYGKKANIAVNGIIHPEKMKNVPEYLAFLKEINVDVITVGDTGIIYTMK
                     KNPELRIPYIYDAETLVTSARQINFWAKKGAVGAVLAREVPFEEMKAMEEKLDIPVET
                     LVYGATCIHQSKRPLLQNYYNYTKQDEQKDRERGLFISEPKKEETHYSIYEDSHGTHI
                     FASNDLNLSNELNELYQHHYCTWKLDGLFTPGEKFVNITRLFAEAKKQILEGSWDQEK
                     AEKMTSEISKLHPENRELDTGFFYIDPSAIK"
     gene            complement(5475..7967)
                     /locus_tag="SCJ86_002503"
     CDS             complement(5475..7967)
                     /locus_tag="SCJ86_002503"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp protease ATP-binding subunit"
                     /protein_id="HEG1170017.1"
                     /translation="MEELFTERAQAVLEIAQEEAKRLKHQSVSSEHVLLALVVEQNGI
                     AGKVLREMDLNETEIYEEIEHLIGYGGIRSYPKGVFLPYSPRMKQVFALASSEVKKTG
                     SQKVGTEHILMSLLKDESIMATRIMINLGISLSKARQVLKQKMGLAGDSAGKGMNRRR
                     NVNVQDKRQTPQGTPTLDSLARDLTKLAKENKLDPVVGRSREVKRLIQILSRRTKNNP
                     VLVGEPGVGKTAIAEGLAQKIILGEVPEDMQEKRLMMLDMGSLVAGTKYRGEFEDRMK
                     KVIDEIYNDGQVILFIDELHTLIGAGGAEGAIDASNILKPALARGELQTIGATTLDEY
                     QKYIEKDSALERRFARIQVDEPTPEEAEVILQGLRTRYEEHHGVEITDEAVRAAVNLS
                     VRYITSRQLPDKAIDLIDESAAKVRLDQTDHLTKSTVIKLEIDELVQEKEAAIQKQDF
                     ENAAQLRRQEKALRKKLQKVSAIEAKQEQGYSDRVTEEDVATVVSEWTGVPLQQLEKK
                     ESERLLELEGLLHERVVGQDEAVKAVSRAIRRARSGLKDPDRPIGSFMFLGPTGVGKT
                     ELAKALSEVMFGSEDALIRVDMSEFMEKYSTSRLIGSPPGYVGYDEGGQLTEKIRQKP
                     YSVILLDEVEKAHPDVFNLLLQVLDDGHLTDSKGRKVDFRNTIMIMTSNIGATQIREE
                     KNVGFNVQDVTKDHKAMQKRILEELKKAFRPEFLNRIDETVVFHSLSKDEIHTIVQIM
                     SKAIIKRLKEQDIQVKITPSAIDVIGKAGFDPEYGARPIRRALQKEIEDRLSEALLGG
                     EIRLGDHVTVGASKGKITLNVRGPKKETVGSL"
     gene            complement(7974..8441)
                     /locus_tag="SCJ86_002504"
     CDS             complement(7974..8441)
                     /locus_tag="SCJ86_002504"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312882.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CtsR family transcriptional regulator"
                     /protein_id="HEG1170018.1"
                     /translation="MAHQNTSDLIESYIKKILENSEMIEIRRIEMADLFNCVPSQINY
                     VINTRFTIQRGYLVESKRGGGGYIRIAKVRISDKKQLLEQINQLFDDTISEKNAFAII
                     QKLYEDQIITKKEGNLMLSAIAKNTLNFNEYEDHTRARILRAFLERLSYEDGK"
     gene            complement(8577..8650)
                     /locus_tag="SCJ86_002505"
     tRNA            complement(8577..8650)
                     /locus_tag="SCJ86_002505"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(8614..8616),aa:Arg,seq:acg)
     gene            complement(8708..8781)
                     /locus_tag="SCJ86_002506"
     tRNA            complement(8708..8781)
                     /locus_tag="SCJ86_002506"
                     /product="tRNA-Pro"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(8745..8747),aa:Pro,seq:tgg)
BASE COUNT         2441 a         1867 c         1461 g         3031 t
ORIGIN      
        1 taattacatg gattatgaaa aggctcattc tgaagtatat aagtatgaag aaaatgtcat
       61 tggccgcaga aatctatctc ctgtattgat tcgaaacatg attttagcta tgattgcatt
      121 tgtttttgtt atcggcgtta ttctaacgat caatacgggt tggcttttac ttttgatggg
      181 atttatctgt tgttttattg gagtattcta tacattcgga ccgattcctt tatctcgaat
      241 gcctttaggg gaaattttca gtggggtgac tatgggcctt ggaatttttg ccatggtcgt
      301 ttatgtcaat acattcggtt cagatgtcta tgatctgaca gtcaactttt catctggaca
      361 atttgatttg gtaggaaatc tttgggctat agcagcaatc gtattagctt ccttaccctt
      421 ggtgtttacg attgccaata tcatgttggc taataatttg cgggatcttg accgtgatat
      481 tgaaaatcat cgctatacac tagtttttta tatcggacgt cctgcgggaa cagtcttatt
      541 ccagctattg atgtatgctt gctatttcgt gattttaatg gggttgttga gcggtatcta
      601 tgaatggccg atcttggtca ccttcttgac cttgccaatt gtgaagaagc atttggatca
      661 attcaagcaa acgttacccc agcctatcag tttcagctat tccatcaaaa atatggtctt
      721 attcaatgcc agttatgtat tggggctatt actaacaatc attatgcaag ccatttaaca
      781 gcatgaagag gttgtgaaaa aactgccctg catcaagtaa taagaacagc caaataaaaa
      841 tagctcctta tatttttcgt ttggttgggc ttattaccgc agatgcaagc ttttgaacac
      901 cgtttatcag gaggttgtga aaaagtcgct tagtttcgag tattaagaaa aattttgtga
      961 aaatggcttt ccacattttt gcataaattt ggcttaatac cgagaaactg gcttttgaac
     1021 accgtttatc agaggttgta aaatcagtgt tttgtcctga gtattagaga aacaaatgga
     1081 aaaatagttc ctcatatttt gccgtttttt gagctaatac cgaaggacag ctgattgaac
     1141 actatttgtt cggaaaaagt gactaactat gacacaattt ttttcacagt cccttttttc
     1201 acttgatttg attcgtacca gcgaacttga cgagaacttt cttggtttgg taactatatg
     1261 gtattctttt aacatcctcg tttgcttctt ctaatttatt tatacaaagt cacacttttt
     1321 taaacatctg ttcttcgata catttctatt tcttttaaac atcgatacca atattttgaa
     1381 cttaagagtg cataccaaat ggattcattt actcttcgtc agttacgaag ataatcaaaa
     1441 tctccaactt ccctacttat ttcgttttga gaattatcga agcaatctta cataaataac
     1501 taaaaaacaa aaaaccagaa aatcagtata ctgattttct ggttttacat ggtttgttca
     1561 ataaaaagct gttggaactg ttaatattaa attatcgcat cgttacgaat tcttccgctc
     1621 ctgttggatg gatggctact gtattgtcaa agtcttcttt agttgcaccc atcttgatag
     1681 ccacggcaaa accttgcagc atctcatcga caccaacacc aatagcatgt aagccgacga
     1741 ttctttcttc ttttccgaca cagatcaatt tcatttcgca tttttggcga taatcattta
     1801 atgcaaagta cattggggta aagcgtgaac gataaacttt caattcattt tcgccatatt
     1861 tttccaatgc ttcttcttct gtcaaaccaa ttgttgcaat tggtggatgc gtaaagacga
     1921 ctgtcggaac aagtttatag tcaagataag aatctttctt tccattaaat aaacgttctg
     1981 ataaacgtct accagctgcg attgcaacag gagtcagatc taatttaccg atcacatcac
     2041 caacagcaaa aatatgttca tccgttgtat tttggtattt atctactttg ataaaccctt
     2101 ttttgtccaa ttccacattt gtattttcca gacctaattt ttcggtattc ggtacacgtc
     2161 caccggcaaa aagtacacaa tcaccagtta aggtttcacc attttcaaat agcaccgtat
     2221 attcgtcatt gtttttagta atttctttag caatatgatg tgcgtaagtt tttattccag
     2281 cttcttggta catttggatc aaattgtccg aaagcatttt atcaaacgtt ctaagtggtc
     2341 gttcttttcg atatgcccac atgacttcac tacctaaacc acgaaataca cctgaaagtt
     2401 cagccgcaat gtatccagca cctaaaacga ttgctctctt aggtaactct gttaattcaa
     2461 aaaatccatt tgaatcaatt gcgtattctc caccaggaat ggtcatttta cttgcacggc
     2521 ctccagtagc gatcaagatt ttaggagcac gataggtttc accgtttact tcgatcgttt
     2581 gactatcgat aaaagtagcg taacctttta tagcctcgac atgattgcta tctaatcctc
     2641 tttgataagc accgtgtaga aaatcaatat atttttctcg tctttctact agaccggcga
     2701 aatcaacatt ttttaaatct gcttggatac cgtaacttga ggcatctcgt tcaattgttt
     2761 ccaaaatcgt acttgcttgc cacataactt ttttaggtac acaaccgacg ttgacacatg
     2821 ttccgcccaa ttcattcgct tcaatcagca aaacttttgc gccatgcatc cctgctctgt
     2881 ttgctgaagc aataccgcca gagccgccac caattacgat gtaatcatat atttgcattt
     2941 tgttcctcct tattgaagag atcaatcaaa acctttattt aacgatcctt tttctacaga
     3001 aaacaagtgt aacacttttc ttgaattttg tatgcgaaga tgcttaaaac agggatatat
     3061 cagctttttc cttatttacg ttttcggatc atatctccag gtttcaccgg ctgtgcgact
     3121 ggcatagtta aaatcatcat tggatttggt gctcggtcaa tggattcgcc ttcttcatta
     3181 tgcataactt caacggtttg actgaaatga ctgaatcctg gaccataaaa ttcgatttca
     3241 tctcctacag aaaacaagtt tctctgacgt atcgtagcaa tctgtgttgc ctcatcataa
     3301 gataatactt caccgacaaa tttatatact gggattttac gacgttcacc gaataattgt
     3361 tcgttttcag ttggtgtatt gtagtaaaaa cctgtagcta attctcgttg tgctactttc
     3421 cataactcgt cgatccattc ttgcttgcag acatagtttt caggatcttc catataactg
     3481 tctacagccg ctttatagac atttgacacc gtagatacat aatggatcga tttcattctt
     3541 ccttcgatct tgaagctgtc tacgccgttt tcaatcaatt ctggaatatg ttcgatcatt
     3601 gacatatcca ctgcactcat cgagaattct tcttccactt cgcctgtatc agttaaactg
     3661 tttcgttctt gaccaaaagg catatcgaac aatccatact tccagcgaca agattgagaa
     3721 catccgccac ggtttgcatc ccgcatcgac atatggttgg acaatgtgca tcgaccagaa
     3781 taagagatac acatggcacc atggataaat gcttcgattt cggtatccgt atttttacga
     3841 atctctgcca cttcctccat tgaaacttca cgagctaaaa cgacacgttc caacccttct
     3901 tctttccaga attccaatgt ctcaaaatta gttgctgaag cttgagtgga taaatggatc
     3961 ggcagacctg gggcttctgt cgcacaaatt tcgatcaaag ctggatcaga aacaatcact
     4021 gcagaaatac cgacatcacg caaactgcgg aaaaattccc ctgctccatc ttcatttcct
     4081 tcatgggtga ccatattagc agcaacgtat accttcgcat catgttcttt tgcaaaagca
     4141 actccttcag ccatttcttc ataagtaaaa ttacctgcac ggcttcttaa accatatgca
     4201 ttcccgccaa tatatactgc atctgctcca taatggatcg cggtttttaa cttttccagt
     4261 gttcctgctg gcgctaggat ttctggtcgt ttcaattttc tttttactgt catttctttc
     4321 atcatactcc ttatttgata gcagaaggat caatataaaa gaatcctgta tctaattcac
     4381 ggttttcagg atgcaattta ctgatttcgc tagtcatttt ttccgctttt tcttgatccc
     4441 agctaccttc taaaatttgt ttttttgctt cggcgaatag tcgagtgatg tttacgaatt
     4501 tttcgcctgg agtaaataat ccgtccaatt tccatgtaca gtaatgatgc tgataaagtt
     4561 cattcaattc attcgagaga ttcagatcat tgctggcaaa aatatgtgtg ccatgactat
     4621 cttcataaat agaataatgt gtctcttctt ttttaggttc cgagataaaa agacctcttt
     4681 cacgatcttt ttgttcgtct tgtttcgtat agttatagta attctgtagc agtggacgtt
     4741 tggattgatg gatgcatgta gcaccataca caagggtttc tacaggaata tcgagttttt
     4801 cttccatcgc cttcatctct tcaaaaggaa cttcacgagc aagtactgct ccaactgcgc
     4861 cttttttcgc ccagaaattg atttgacgtg cgctcgttac taatgtttca gcatcataaa
     4921 tatacggtat acgcaattca ggattttttt tcatcgtata aataatacct gtatcaccaa
     4981 ctgtgatcac atctacgttt atttctttta aaaaagccaa atattcaggt acatttttca
     5041 ttttttcagg atgaatgatc ccatttacag caatattcgc ttttttacca tacttgtgtg
     5101 ctaattctac caattgtcgc tgttcttctc gtgtaaatga agcaggcaga cgcaatccaa
     5161 attgctcttc accgaaatat agtgtatctg cacctgcagc aagcaactgt tctgcttgtt
     5221 caatagtttc tactgtggtt atgagttcga tcataatttt ctcctcctac aacttcataa
     5281 tagtagcata gctctaaaaa ctttccaata cttcttctat ttaaagtcgg atttttgaaa
     5341 attatttcac aaaaaatcaa aataaagttc gatatattct gtccaaatat aaaaagagat
     5401 tgtgacaaaa atcttgtcac aacctcttaa tcaagtctta ctattataaa aattcagcta
     5461 cacaggccaa gtaactatag ggaacctact gtttcctttt taggtccgcg aacattcaat
     5521 gtgatcttac cttttgaagc acctactgtt acgtggtcac ctaaacgaat ttctccgcca
     5581 agcaaagcct cgctcaatcg atcttcaatt tctttttgaa gtgcgcgacg gattggacgt
     5641 gcaccatatt caggatcaaa tccagctttc ccgatcacat cgattgcaga tggtgtgatt
     5701 ttcacttgaa tatcttgttc tttcaatcgt ttgataatcg ctttgctcat gatttggacg
     5761 attgtatgga tttcatcttt actcaatgaa tggaagacga cagtctcatc gattcgattc
     5821 aagaattcag gtcggaaagc tttctttaac tcttctaaga tacgtttttg catggctttg
     5881 tgatctttag taacatcttg gacattgaat ccgacatttt tttcttctcg aatttgtgtc
     5941 gctccgatat ttgaagtcat aatcatgatt gtatttctaa agtccacttt tctaccttta
     6001 gaatctgtta aatgaccatc atccaatact tgcagaagta aattgaaaac gtctggatgt
     6061 gctttctcca cttcatctaa gagaatcact gaatatggtt tttgacgaat tttttctgtc
     6121 aactgtccac cttcatcata ccctacgtat cctggaggag acccgattaa tcggcttgta
     6181 ctgtattttt ccataaattc tgacatatca acacggatca gggcatcttc acttccaaac
     6241 atgacttctg aaagtgcttt ggccaattct gtttttccga caccagttgg tccaaggaac
     6301 atgaatgaac caattggacg atcaggatct ttcaatccgc ttcgtgcacg gcggatcgca
     6361 cgtgaaactg ctttgacagc ctcatcttga ccaacgacac gttcatgcag aagtccttcc
     6421 agttctaaca agcgttcgct ttcttttttc tccaactgct gtagagggac accagtccac
     6481 tccgatacaa ctgtagcaac atcttcttct gttacacgat cagaataacc ttgctcctgt
     6541 tttgcttcaa ttgcagaaac tttttgcagc tttttacgta gtgctttttc ttgtctgcgt
     6601 aattgagcag cattttcaaa gtcttgtttt tggatcgctg cttccttttc ttgtactagt
     6661 tcatcgattt ctagtttgat cacagttgat tttgttaagt gatcggtttg atccaaacgg
     6721 actttcgctg ctgattcatc gatcaaatca attgctttat cgggtagttg acgagaagtg
     6781 atataacgaa cagaaagatt gactgctgct cgtacagctt catctgtgat ctccactcca
     6841 tggtgctctt catatcttgt acgtaatcct tgaagaatca cttctgcttc ttcaggagtt
     6901 ggctcatcta cttgaatgcg agcaaaacgt cgctctaatg cagaatcttt ttcgatatat
     6961 ttttgatatt catctagggt cgttgctccg atcgtttgta attctcctcg agctaacgcc
     7021 ggtttcaaga tattcgaagc atcaattgct ccttctgctc cacctgcacc aattaaggta
     7081 tgcaattcat caatgaataa gattacttga ccatcattat agatttcgtc gatgaccttt
     7141 ttcattcgat cttcaaattc accgcgatat ttcgtaccag caaccaaaga tcccatatca
     7201 agcatcatca ggcgcttttc ttgcatgtct tctggcactt cacctaaaat gattttttga
     7261 gctaaccctt cagcaatggc tgttttgcct acaccaggtt cacccaccag tacagggtta
     7321 ttctttgtcc gtcggctaag aatctggata agccgtttca cttctctcga acgaccaaca
     7381 actgggtcca gcttgttttc ttttgctaat ttcgtcaaat cacgtgctaa tgaatctagc
     7441 gtaggtgttc cttgcggtgt ttgccgttta tcttgtacat ttacatttct acgacgattc
     7501 attccttttc cagcagaatc accagctagc cccatttttt gtttcagaac ttgacgggct
     7561 tttgaaaggc tgatacctaa gttgatcatg attcgagtag ccatgatact ttcatctttt
     7621 aataagctca tcaaaatatg ttctgttcca actttttgag aaccagtttt cttcacttcg
     7681 ctgctagcta aagcaaaaac ttgtttcatt cttggtgaat atggaagaaa gactccttta
     7741 gggtaagaac ggataccacc ataaccaatc agatgttcaa tttcttcata aatttccgtt
     7801 tcattcaaat ccatttcacg taatactttt ccagcaattc cattttgctc gaccactaag
     7861 gctagtaaaa catgttctga actaactgat tgatgtttca accgttttgc ttcttcttgt
     7921 gcaatttcta atactgcttg ggctctttca gtaaaaagtt cttccatcta atctcacttc
     7981 ccatcttcat aacttaaacg ttccagaaat gctcggagaa tccgagcacg tgtatgatct
     8041 tcatattcat tgaaattcaa tgtgtttttt gcaattgcac tcaacatcaa gtttccttct
     8101 ttttttgtaa ttatttggtc ttcataaagt ttttgaataa tagcaaaagc atttttctca
     8161 ctgattgtat catcaaataa ctgatttatc tgctcaagta attgtttttt atctgaaatc
     8221 cttacttttg caattcgtat ataaccacca ccaccacgtt tgctttctac tagataacca
     8281 cgttggatgg taaaacgggt attaatcaca tagttgattt gtgaaggaac acagttaaag
     8341 aggtctgcca tttcaatcct tcgaatctcg atcatttcac tattttccag aattttcttt
     8401 atatatgatt caattaaatc cgaggtattc tgatgtgcca tcttattcat tccctttgac
     8461 taatattgac cttaattata acgaatttga taatagaaag aaagaagaac gctttttgtt
     8521 aaataaatcc tttagaatca acaaaaaaaa atacggaata tactattccg tatcgacgcg
     8581 cccaagagga gtcgaacccc taaccttttg atccgtagtc aaacactcta tccagttgag
     8641 ctatgggcgc atattcaatt taaaaaaatt ataaatcatt tgaaaacaaa aagcaagata
     8701 aaaaaaatcg ggaagacagg attcgaacct gcgacccctt ggtcccaaac caagtgctct
     8761 accaagctga gctacttccc gtttaaaaaa taaaaaagac
//
LOCUS       DARBET010000095         8675 bp    DNA     linear   BCT 07-NOV-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4027
            SAMEA114569841-rid20112183.denovo.097, whole genome shotgun
            sequence.
ACCESSION   DARBET010000095 DARBET010000000
VERSION     DARBET010000095.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA114569841
            Sequence Read Archive: ERR12203666
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 8675)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8675)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001972618.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001972618.1
            Genome Coverage       :: 132x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/07/2023 13:18:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,041
            CDSs (total)                      :: 2,975
            Genes (coding)                    :: 2,908
            CDSs (with protein)               :: 2,908
            Genes (RNA)                       :: 66
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 67
            CDSs (without protein)            :: 67
            Pseudo Genes (ambiguous residues) :: 0 of 67
            Pseudo Genes (frameshifted)       :: 26 of 67
            Pseudo Genes (incomplete)         :: 28 of 67
            Pseudo Genes (internal stop)      :: 20 of 67
            Pseudo Genes (multiple problems)  :: 6 of 67
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8675
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA114569841-rid20112183.denovo.097"
                     /isolate="VRE4027"
                     /db_xref="taxon:1352"
                     /country="Denmark"
     gene            <1..1101
                     /locus_tag="SCJ86_002507"
     CDS             <1..1101
                     /locus_tag="SCJ86_002507"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ISL3 family transposase"
                     /protein_id="HEG1170019.1"
                     /translation="KKETIVRFEQYNHMPLVTRLKKQRYTCKNCRTHWTAQSYFVRLS
                     HSIADHVRYKIASLLTEKVSLSFIAKSCQVSLTTVTRTLKEFKSYLPKPSKKILPRVL
                     MVDEFRSHASIEDKMSFICADGETGNLIAVLPTRKLPRLTSYFLGCTNPEEVEFLVTD
                     MNAAYFQLTKRVLPNAKVVIDRFHIVKHMNQALNELRIREMNELRKAGQKSQAEKLKK
                     NWRFLLKNRASINHYEYKTWKSFRAPKYPFLTEAMMIDRLLEFSAPLKEAYPFFHELV
                     EAFRDKDPDLFFSLLAELPETLDDGFREKLQNLLTYEEGITSAMIYPYSNGKIEAKNT
                     HIKTMKRVSYGFKSFENMRIRIFLINQLINVR"
     gene            complement(1280..1609)
                     /locus_tag="SCJ86_002508"
     CDS             complement(1280..1609)
                     /locus_tag="SCJ86_002508"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326660.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system PemK/MazF family
                     toxin"
                     /protein_id="HEG1170020.1"
                     /translation="MNTNDIATVYVAFTAAEGGKRRPVYILKNGLDTISFLSITSKYH
                     TKSDKIKLKYVEIRDWKQAGLKKESWIDVGTLNELPKNNKKVKWKKIGELSPSDLQRL
                     EERMEEL"
     gene            complement(1599..1871)
                     /locus_tag="SCJ86_002509"
     CDS             complement(1599..1871)
                     /locus_tag="SCJ86_002509"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299921.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelB/DinJ family
                     antitoxin"
                     /protein_id="HEG1170021.1"
                     /translation="MEKMAKLQISMDEKLKNEAESIIDELGMSPKTAVTVFYKQIVEQ
                     GKIPFSIELSEKSKRIHNIQRLAEKLPVQKLDTDEKIEEWFNEDEY"
     gene            complement(2322..3125)
                     /locus_tag="SCJ86_002510"
     CDS             complement(2322..3125)
                     /locus_tag="SCJ86_002510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299923.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase"
                     /protein_id="HEG1170022.1"
                     /translation="MRFLHLSDIHFRENYQTNLFTAELFPTIKNPQLALEKLLQELSL
                     TVYDFILLTGDLVHEGGISDYQVLRKMLDQYFPTTPYYFCRGNHDRRSVFFEGMSVES
                     NETGEYIHCTNQNGLRIISLDSAQDTQHEGKISIGQMIQLQEWLGTPAEKGTILLVHH
                     PLSWEDPSIVTEVPEGFAELIAESDILGIFVGHIHQGSSAEFAGKKQFMAESLSFGVD
                     EYPDYSLFTNRTGFTSGSLIDGKIFTYQHYLTPHQIEIGRMKKPITGNH"
     gene            complement(3103..4227)
                     /locus_tag="SCJ86_002511"
     CDS             complement(3103..4227)
                     /locus_tag="SCJ86_002511"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299925.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEG1170023.1"
                     /translation="MGETLELRNIGKKYGEHTILKEIDLAVAAGERIVLLGPSGSGKS
                     TLLRMIAGLEKITSGELFLGTQLANKLPCGEREIAMVFQNYALYPHMTVKENIIFALR
                     ANKIKTADIQHRLEEALKMLGLTPYQDRLPKDLSGGQRQRVALARAVVKRSNYFLLDE
                     PLSNLDVRLRLDARKELVKIHQRYKQTFVYVTHDQIEAMTLADRIVLLNEGRIQMIDT
                     PKNVYTKPKNTFTANFIGSPGMNIMPVTYDQGTLKVASQFLTLNPAWQKQLLQVSGSL
                     YLGIRPEHVTFSKETSDLHGQVKYRELLGQSYAYTVKVDGIEMIVLNEKPTWQIGDAV
                     NLRIEPERMHFFDYQTQENLGYPDYFIEERGNSHALFASL"
     gene            complement(4243..5058)
                     /locus_tag="SCJ86_002512"
     CDS             complement(4243..5058)
                     /locus_tag="SCJ86_002512"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299926.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HEG1170024.1"
                     /translation="MKIKRWLPHLLFMFIIIIIIYPLLFIMANSFKPLKDAYQTITSL
                     IPQPFTLENFCYLFENLPIIKITRNTFFIATIITVVKLGVAFFAAYSFTYCRIKGKKI
                     WYFIIIGTMFIPFTVAMIPNYLLIAKLNLIDTTWGVIIPQFADAMGIFLLTQTMREIP
                     VSLLDVAALDNIKQTTVMSKIVFPLTRHAITSTGIWFFITSWNEYVWPVLILRTVDNY
                     TLPLAMQTYISSEGGTNFTVAMAISLITMFVPLVLYAIFQKYIIGTFVSAGIK"
     gene            complement(5048..5920)
                     /locus_tag="SCJ86_002513"
     CDS             complement(5048..5920)
                     /locus_tag="SCJ86_002513"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006739050.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HEG1170025.1"
                     /translation="MKRYVGYLYLAPAIILLGVFVFYPLIYTIYLSFFDWNMVAPTKE
                     FVGFANYVKVLTDPVFHKVLKNTLSYTVLFVLLSCVFPYITSFLLGIVIEKGQNLYKP
                     LFFIPAVISLVVAGMVFTWILNPVSGPIAVLLGNFGLRLPMWMNLQGWVIAVIAGITA
                     WKVFGYNFIVIFAAIVGIDQEMIEAAKLDRVPIWRIFFTIVLPMSSSTGLYLLVMTIV
                     QGLQYVFTPIKIITRGGPNNGSANVIYYAYQKAFEMYKVGESSAISVLTLLIFAVFLG
                     VIFKFVEKGVYYEN"
     gene            complement(6005..7294)
                     /locus_tag="SCJ86_002514"
     CDS             complement(6005..7294)
                     /locus_tag="SCJ86_002514"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299928.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HEG1170026.1"
                     /translation="MLKKNLVIGVVSVLSVISLAACGSGNTKTTKKSEGRVTIEYWHV
                     NADTLGGKTVNELVDKYNQSQDKVKVVAKYNPDMYKGLMQNLQASVASKEIPDVVQVG
                     WAFKDYFAENFKYTNPQSLVEEVDPDNADYFKDHFLDNIMALSEKDGKQVGIPYSISN
                     PVLYLNQDILKAAGLDENGPKTWEEVQTFSKIIKEKTGKYGVYIQEPADNWAQQAILE
                     SNGASVIKDGKATFASKDGIAAYELYQRMVKDQTALHTTWEQGVQSFIDGNVAMVYTT
                     IAQRDNIQKNSTFTAKAVESPSWEGKKVKLPAGGAMLAVTSQTDKKKEATWDFLKYLY
                     SVESLAKWTEGTGYLPPRKDVADAQNGLKAFLAENQMMIPAIDQMDKMVSWSSFPGDA
                     GLEAEQKLLDMRDKILSGNSVNETMKATQKEINELLK"
     gene            complement(7522..8166)
                     /locus_tag="SCJ86_002515"
                     /pseudo
     CDS             complement(7522..8166)
                     /locus_tag="SCJ86_002515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303552.1"
                     /note="internal stop; incomplete; partial in the middle of
                     a contig; missing C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="glycerophosphodiester phosphodiesterase"
BASE COUNT         2691 a         1850 c         1371 g         2763 t
ORIGIN      
        1 aagaaggaaa cgattgtccg ttttgaacaa tacaatcata tgcctttggt tacgcgccta
       61 aaaaagcaac gctatacctg taaaaactgt cgtacccatt ggacggctca aagttatttt
      121 gtccgactca gccattcaat cgcagatcat gttcgatata aaattgcttc tttactgact
      181 gaaaaagtat ctttgtcttt tattgcgaaa agttgtcagg tatctttgac taccgttact
      241 cgtacattga aagagtttaa aagctattta ccaaagcctt ctaagaagat tctccccaga
      301 gtattgatgg ttgatgaatt tcgttcgcac gcttccatag aagataaaat gagctttatt
      361 tgcgcagatg gtgaaacagg aaacttaatt gctgtcttgc ctacgcgtaa attacctcga
      421 ttaacaagct atttcttagg ctgcaccaat ccagaagaag tagaattctt agtcacagac
      481 atgaacgccg cttacttcca gcttaccaaa cgtgttctgc caaatgcgaa agtagtgatt
      541 gatcggtttc atattgtcaa acacatgaat caggcactca atgagttgcg tatccgtgaa
      601 atgaatgagc ttcgtaaagc aggacagaaa agtcaggcag aaaaactgaa aaagaactgg
      661 cgctttctgc taaaaaatcg tgcaagcatc aaccattatg agtacaaaac atggaaaagt
      721 ttccgagcac caaaataccc atttctcact gaagcaatga tgattgatcg attgcttgag
      781 ttttctgcgc ctctaaagga ggcgtatccc ttttttcatg aattagtcga agcctttcga
      841 gacaaagacc ctgacttatt tttctcctta ttggcagaac ttcccgaaac gttagatgac
      901 ggctttcggg agaagcttca aaaccttctg acctatgaag aaggtatcac cagcgcaatg
      961 atctatcctt attccaatgg aaaaattgaa gcgaagaata cccacataaa gacaatgaaa
     1021 cgagtatcct acggatttaa atcattcgag aacatgagaa ttagaatctt tttgatcaat
     1081 caattaatca atgtaagata acaaaaaatc tgaaccagaa tgtggttcat tctgacccgg
     1141 atttgatttt gcacaactca tcagtcctta ttgacaaaga gccaaaaatc tgagccagaa
     1201 tgaggttcat tctgacccag atttgatttt gtacaaccca tcagtcctta ttgacaaaga
     1261 gcccttcttt ttagtattat tataattctt ccatgcgttc ttccagtcgc tgtaaatcgc
     1321 ttggacttaa ttccccaatt tttttccatt tgaccttttt gttgtttttg ggtaattcgt
     1381 ttagagttcc tacgtcaatc caagattctt tttttagtcc tgcctgtttc caatccctta
     1441 tttcaacgta tttcagcttt attttatcgg atttagtatg atattttgaa gtaattgaca
     1501 gaaacgaaat tgtgtccagt ccatttttta gaatatagac aggccggcgt ttaccgccct
     1561 ctgcagctgt gaaagcaacg tagacggttg ctatatcatt agtattcatc ttcattgaac
     1621 cattcttcaa ttttttcgtc agtgtctagt ttttggacag gcaatttttc cgctaatctt
     1681 tgaatattat ggattcgttt acttttttcc gacaattcaa tagaaaatgg gatttttcct
     1741 tgctcaacaa tttgcttata aaaaacggtg acggctgttt tggggctcat gcctagttca
     1801 tcaataatgc tttctgcttc atttttcaat ttttcgtcca tagaaatttg taacttagcc
     1861 attttctcca cctctttctg acaaacacat tatatcacgg aaagagttca aatggaatta
     1921 atacaactac ttttgaactc cttgaaatgc agtgtattta tctaaatata aggaaaggat
     1981 atagtgctaa atggccttga tagatagaaa agtttcaaaa tccgccacag cgataattga
     2041 agcgaacact caacgacaca cagaacagga aaagactcct aaaagaacca aattaactag
     2101 gacgtgtctg aaaactccac aaagatgtgg tatgctctgg taaaaaacga atggcacaac
     2161 cacgaagacc gatatggttc ttggaaaact ttctccaccc tttttgtaat tggagggata
     2221 gtggtttatt gatagcgatt ttccaagttc ttcaagtgga accagacatt gagcaccacg
     2281 gctaaaaagt gaatgaagtc tttattttta atcaataatt tttaatgatt accagtaatt
     2341 ggttttttca tgcgccctat ttctatttga tgtggagtta aataatgctg ataagtaaaa
     2401 atttttccgt caattaaact gccactagtg aatcctgttc tattggtaaa aagagaatag
     2461 tctgggtatt catctacacc aaatgataaa gattcagcca taaactgttt tttaccagca
     2521 aattcagcac tgctaccttg atggatatgt cctacaaaaa tacctaaaat atcactttcg
     2581 gcaatcaatt cagcaaaacc ttccggaact tcagttacaa tagatggatc ttcccacgac
     2641 aatggatgat gaaccaataa aatcgttccc ttttcagcag gtgtacccaa ccactcttgt
     2701 aattgaatca tctgtcctat cgatattttt ccttcgtgct gggtatcttg agcagaatcc
     2761 aaggaaataa ttcgtaaccc attttgattc gtacaatgga tatactctcc cgtttcattg
     2821 ctttcaacac tcattccttc aaagaaaaca ctgcgacgat catgattccc acgacaaaaa
     2881 tagtaaggtg tggttgggaa atattgatcc aacattttcc gtaagacttg ataatcagaa
     2941 atcccccctt catgtactaa atcacctgtt aatagaataa aatcgtacac tgttaacgag
     3001 agttcttgta acaatttttc caatgccaac tgtggatttt taattgttgg aaatagttca
     3061 gcagtaaaca agttagtttg atagttttct ctaaaatgga tatcagagag atgcaaaaag
     3121 cgcatggcta ttccctcttt cttcaataaa ataatcagga tatcctaaat tttcttgcgt
     3181 ctgataatca aaaaaatgca ttctttcagg ttctatgcgg agattgactg catcaccaat
     3241 ctgccaagta ggtttttcat taaggacaat catttctata ccatcgactt taacggtgta
     3301 cgcataactt tgacctaata gctcacgata cttcacttga ccgtgtaaat cgcttgtttc
     3361 ttttgaaaag gttacgtgtt ctggtcttat gcctaaatac aatgagccac taacctgaag
     3421 aagttgtttt tgccaagctg gattcaatgt taaaaattga ctggctacct ttaatgttcc
     3481 ttgatcataa gtaactggca taatattcat gcctggcgaa ccaataaaat ttgcagtaaa
     3541 agtattttta ggtttcgtat aaacattttt cggcgtatca atcatctgaa ttctaccttc
     3601 attcaataaa acaatccggt cagctaacgt catcgcttca atttgatcat gagtgacgta
     3661 gacaaaagtt tgtttataac gttgatgaat ttttaccaat tcttttcgtg catccaatcg
     3721 taaacgcaca tccaaatttg ataacggttc atctaataaa aaataattac tccgtttaac
     3781 caccgcacga gccaatgcta cacgctgccg ttgccctcct gacaaatctt taggaagtcg
     3841 atcttggtat ggcgttaaac ctaacatctt caaagcttct tccaaacggt gctgaatgtc
     3901 agctgtctta attttattcg cccttaatgc aaaaataata ttctccttta cagtcatgtg
     3961 aggatataaa gcataatttt gaaaaaccat tgcgatctct cgttctccac aaggtagctt
     4021 atttgctagt tgtgtaccta aaaaaagctc tccactcgta attttttcca aaccagcaat
     4081 cattctcagc aaagtagatt ttcctgaacc agatggtccc agtaacacaa ttcgttctcc
     4141 agcggccaca gctaaatcaa tttcttttaa aatggtatgt tctccatatt tttttccgat
     4201 atttcttaat tcaagcgttt ctcccatgat ttgtcctcca atttatttaa tacctgcaga
     4261 aacaaaagtc ccgataatat atttttgaaa aattgcatac aagactaacg gtacaaacat
     4321 tgtaattaaa ctaatcgcca ttgctacggt aaaatttgtc ccaccttcag aactaatata
     4381 ggtctgcatc gccaacggca aagtataatt atctactgtc cgtaaaatta ataccggcca
     4441 aacatactca ttccaagagg taataaaaaa ccaaataccg gtagaagtaa tcgcatgtct
     4501 agtaagagga aaaacaattt tactcatgac ggttgtttgt ttgatgttat ccaatgccgc
     4561 cacatctaga aggctcactg gaatttcccg cattgtttgc gtcaataaaa aaatgcccat
     4621 cgcatcagca aattgtggta ttatcacacc ccaagtagta tcaattaaat taagttttgc
     4681 aatcaacaaa taattaggaa tcatcgcaac agtaaaagga atgaacatcg taccaatgat
     4741 gataaagtac cagattttct tccctttgat tcggcaataa gtaaaactat atgccgcaaa
     4801 aaaagctacg cctaacttaa caaccgttat aattgtagca ataaaaaaag tatttctcgt
     4861 aattttgata attggaagat tttcgaacaa atagcaaaaa ttttccaagg taaacggctg
     4921 tggtatcaaa ctagtaatcg tctgataggc atctttcaat ggcttaaaag aattggccat
     4981 aataaacaat aatggataga taataatgat tatgatgaac ataaataaga gatgtggcaa
     5041 ccagcgttta attttcatag taaaccccct tttccacaaa tttaaatatt acccctaaga
     5101 aaacagcaaa tattaataaa gttaaaactg aaattgctga agattctccg actttataca
     5161 tttcaaaagc cttttgataa gcataataga tcacgtttgc actaccatta ttcggtccac
     5221 cacgagtgat gatcttaatc ggagtaaaca catactgcag accttgaaca atggtcatca
     5281 ccaataaata tagtcctgtc gaactactca taggcaacac aattgtaaaa aaaatgcgcc
     5341 agatgggtac acgatccaac ttagctgctt cgatcatctc ttgatcaatt cccacaattg
     5401 ctgcaaaaat gacaatgaaa ttataaccaa aaactttcca agcagtaata cctgcaatca
     5461 cagcaattac ccaaccttgt aaattcatcc acataggcag tcttaaacca aaatttccca
     5521 acaagacagc aattggtccc gataccggat ttaatatcca tgtaaaaacc atccccgcca
     5581 ctaccaaaga aatcacagct ggaataaaaa ataatggttt atacaaattc tgcccttttt
     5641 ctataactat tcctaataaa aaggatgtaa tatatgggaa cacacagctc agcaagacaa
     5701 atagaacggt ataggacaaa gtattcttca acactttatg aaagactgga tcagtcagca
     5761 cctttacata gttggcaaag ccaacaaatt ctttagtggg ggccaccata ttccagtcaa
     5821 aaaaactcaa atagatcgta taaatcaatg gataaaaaac aaacacgccc aacaaaataa
     5881 ttgctggcgc cagatacaaa taacctacat agcgcttcat ttccgacagc ctcctcatcc
     5941 ttatgtcaaa agcagacgac cttgtttttc ctttcttaca ggcgctgtcg tctgcttgct
     6001 ttgcttattt gagtaactca ttaatttcct tttgagtagc tttcatcgtt tcattgacgg
     6061 aattacctga taaaatttta tcccgcatat ccaacaattt ttgttcagct tctaatccag
     6121 catctccagg aaatgaactc caactgacca tcttatccat ctgatcaatt gctggtatca
     6181 tcatctgatt ttcagctaag aaagctttta atccattttg ggcgtctgca acatctttac
     6241 gtggtggtaa ataaccagta ccttctgtcc atttagctaa agattcaaca ctataaagat
     6301 attttaagaa atcccaagtt gcttcctttt tcttatccgt ttgtgatgta acagctaaca
     6361 ttgcgccacc agcaggtagt ttaacttttt tcccttccca acttggtgac tcaactgctt
     6421 tagcagtaaa agtactattt ttttgaatgt tgtccctttg agcaatagtg gtatagacca
     6481 tcgcgacgtt accatcaata aagctttgca caccttgttc ccacgtagtg tgtaatgccg
     6541 tctggtcttt taccatcctt tgatataatt cataagcagc gataccatct ttagaagcaa
     6601 aggtagcttt gccatctttt attacacttg ccccattact ttctaaaatt gcttgttgtg
     6661 cccaattatc agcaggttct tgaatataca cgccatactt tccagttttt tcttttatta
     6721 tcttagaaaa agtttgaact tcttcccaag tttttggacc attttcatcc aagcccgctg
     6781 cttttaatat atcttgattt aaatataaaa ccggattact aatggagtaa ggaataccca
     6841 cttgtttacc atctttttca gacagtgcca tgatattgtc taaaaaatga tctttaaaat
     6901 aatcagcatt atccggatcc acttcttcta ctaaagattg aggattagta tatttgaaat
     6961 tttctgcaaa ataatcttta aaagcccatc ccacttgtac tacgtccggt atttctttgg
     7021 aggcaacaga agcttgtaaa ttttgcatca agcctttata catatcagga ttatattttg
     7081 ctacaacctt cactttgtct tgagattgat tgtatttatc tactaattca ttcactgttt
     7141 ttccacctag ggtatccgca ttaacatgcc aatactctat cgttacacga ccctccgatt
     7201 tttttgttgt cttggtattc ccactaccac aagctgccaa agagatgaca cttaaaacag
     7261 acacaacccc tataacaaga tttttcttta acataacatt aacttcctcc taatttcttt
     7321 atggccattt ttctaatcaa gaaatattat gaactaccaa tgtaaatttc tagttaattt
     7381 tctgtaaata attcgaaatt tgtgcatttc ggggtacagg gtcactggac atatccatca
     7441 agctaagaat aaagccccca tatggtcatg tatcccatct ccaaatctct taactcctct
     7501 gttttgatta taaaatagtt tattctgtgc tcctttcttc cataaatagg cccatgcaca
     7561 acgtgaggtt atattataaa caccacagcg cgcaaggtac tcaatcgttt ctagttcatt
     7621 gactgtccat acagaaaatg tcaatcctag ctgctgcccc acttcacggg ccaacttagt
     7681 taaccatcct tcatgaatat tgacgatttg aacgctttga taggctaatt tctgtaaggt
     7741 atcgatcaaa tctgcttcgg taatatcccc aagttttttc ccttcgaact gattttcaat
     7801 attataaaat atatgctttc cccaaatcgc agcccaagat aattgtacat agccactaag
     7861 aacaatttgt ggccagattt gataggtttg tgccagcgaa aaaacggcca tttccaatcc
     7921 ctcttctttt aaatcacagt taatggtcat ggttgccttc tcattggcca ctgcttgaaa
     7981 cacctgctca agggttaccg aatctgggac aacttcttac ctgtcatggg ctagtattag
     8041 cgtaccatct gtttgctgac gcacatctac ttcaaaacaa agctggcgat ccaaacaaaa
     8101 gcgaagaaat gcccagctat tctcctccgt attgtcacta ccagagtgtg cagtgatcat
     8161 ggtcattttg acccctcctt taattccgtg attttctccc acacgtccac tataccgatg
     8221 aagcgttaat ttcttgttaa tttcttgtaa attccctgcg ttttttcagt aaactagttg
     8281 taaatcttgc atgctccttc ctacgaactc atgaatcgtc gattttgccc ttctagctag
     8341 tttcaccaat taagtagttc tacattcccc gcttttaacc tactacacct ttttttgttt
     8401 tcagctattc cttcttcttg ctgaaagtcc ttgaggttct ttcttacagg ataccatcaa
     8461 ggtgttcaat ggcttattcc cattgaatga ccgagactta ctatcagatt tctgctcgtt
     8521 gaaccaagtc tttgatacag gatttcttcc gtcatcttct cacaaatacc accttgcgtt
     8581 tctctagcga ttggtcgatg tgcaccctcc cagtggactg tcactacccc tttttgctat
     8641 gcctagccca caaaaaaagc tgtcctcaca gacag
//
LOCUS       DARBET010000096         8586 bp    DNA     linear   BCT 07-NOV-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4027
            SAMEA114569841-rid20112183.denovo.098, whole genome shotgun
            sequence.
ACCESSION   DARBET010000096 DARBET010000000
VERSION     DARBET010000096.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA114569841
            Sequence Read Archive: ERR12203666
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 8586)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8586)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001972618.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001972618.1
            Genome Coverage       :: 132x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/07/2023 13:18:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,041
            CDSs (total)                      :: 2,975
            Genes (coding)                    :: 2,908
            CDSs (with protein)               :: 2,908
            Genes (RNA)                       :: 66
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 67
            CDSs (without protein)            :: 67
            Pseudo Genes (ambiguous residues) :: 0 of 67
            Pseudo Genes (frameshifted)       :: 26 of 67
            Pseudo Genes (incomplete)         :: 28 of 67
            Pseudo Genes (internal stop)      :: 20 of 67
            Pseudo Genes (multiple problems)  :: 6 of 67
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8586
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA114569841-rid20112183.denovo.098"
                     /isolate="VRE4027"
                     /db_xref="taxon:1352"
                     /country="Denmark"
     gene            complement(45..1511)
                     /locus_tag="SCJ86_002516"
     CDS             complement(45..1511)
                     /locus_tag="SCJ86_002516"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288052.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide MFS transporter"
                     /protein_id="HEG1170027.1"
                     /translation="MEKQQDKGFFGQPKGLSTLFFTEMWERFSYYGMRALLIYYMYDT
                     VANGGLGLPRATALAIMSIYGSLIYMSSIVGGWVSDRVLGAKRTVFFGGVFIMLGHIV
                     LALPFGVSTLFVSMALIIIGTAFLKPNVSGMVGHLYSKTDLRRDAGFSIFYMGVNLGA
                     LLAPLIVGTIGQEYNYHLGFSLAAIGMFFGLLQYIIQGRKTLDGVGTTPPNPLTTEER
                     KGFIRNLIIALVVIAAVFGGAQLTGHLTIDFFVNTISVLGIILPLYYFIKMLTSKDVT
                     AEEKPRVWAYIPLFLAAIIFWSLQEQGSSILALFASERTQSTLFGVTIQASWYQSLNP
                     IFIFILTPVFVTMWTKLGERQPSTVVKFSLGLLFAGLSYLLLMLPGMLYGTSGKVSPL
                     WLLGSFFIVVIAELCLSPVGLSVTTKLAPRAFESQTIAIWFLADASSQAINAQIARFY
                     TPGTESAYFGIIGAVAIVGGILLFTVKEPIKKLMGSIH"
     gene            1898..2494
                     /locus_tag="SCJ86_002517"
     CDS             1898..2494
                     /locus_tag="SCJ86_002517"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288054.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitroreductase family protein"
                     /protein_id="HEG1170028.1"
                     /translation="MTTFTDTLKNRRSIYHLGRNVSLSNEELTTLIKEAIKESPTAFN
                     AQSTRAVILFGDAHEKLWEITEEALRPLTPAEAFPNTQNKLAGFKNGYGTVLFFKDTD
                     VVKGLQEQFELYADNFPDWSEQSNGIATANTWVALVDKGLGANLQHYNPVIDEAVAKE
                     WNIPSNWKLRSQLVFGSPETPAGEKEYMNDADRFRVFG"
     gene            complement(2552..3034)
                     /locus_tag="SCJ86_002518"
     CDS             complement(2552..3034)
                     /locus_tag="SCJ86_002518"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292941.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEG1170029.1"
                     /translation="MVTFFTVLLFLFVVVMFLFLWLVRKEIIYRTVRNRWVYLVIPFL
                     VSLVIWYTLISHPTADELAKGILSAMIFISFLLDSRGITEEGLVLNSFDKKGVPFSEI
                     NKIVLYQPKGSKIVKMNFFRNGWRGPMLKFSASLEELVPFLSQRLNDEAEIDIMIDPE
                     "
     gene            complement(3140..3337)
                     /locus_tag="SCJ86_002519"
     CDS             complement(3140..3337)
                     /locus_tag="SCJ86_002519"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325694.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEG1170030.1"
                     /translation="MTEEMINLGEQYACKPIGFTKTVIGEVVSKMTNCAVVKVAQCAA
                     EDQELLDEKASMVVAKYDTFE"
     gene            3632..4390
                     /locus_tag="SCJ86_002520"
     CDS             3632..4390
                     /locus_tag="SCJ86_002520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="C39 family peptidase"
                     /protein_id="HEG1170031.1"
                     /translation="MKKKTFKKLLFLGGTAALLLSGTSYYLHIHSPEHTMNTAQKSST
                     QKTKKTKEKAEVLLDVPLESQFDSPSLENGCEVTSLSMLLSFYGYETTKNQLAEQLDY
                     VPVFNADGTHGDPNEGFVGDISGGDWAMVVYVPPVASLAQQIVQTDYHTFPKTDAQLD
                     DIKKALSKGNPVWTSVTINFEVPDETDFMTWTTNNGEVRVTPLVHACVVTGYDKENIY
                     VNDPYGVKNRAVPINDFSAIFTAMGGQMLTLEKS"
     gene            4450..4878
                     /locus_tag="SCJ86_002521"
     CDS             4450..4878
                     /locus_tag="SCJ86_002521"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295776.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEG1170032.1"
                     /translation="MEKFTHKKMDPNEIPIIFVRDCKGNVQGKVSINEWNERRRPATL
                     NELEIKLYRQSLVYYADQEYEKATDLLKFLIARTEYTRFEYIERLANIYHIMNEPVKE
                     YQLLDTVLSVAELIALPAGLEKKLVRRLLRVKQQLSDQEK"
     gene            4924..4996
                     /locus_tag="SCJ86_002522"
     tRNA            4924..4996
                     /locus_tag="SCJ86_002522"
                     /product="tRNA-Gln"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:4957..4959,aa:Gln,seq:ctg)
     gene            complement(5146..5517)
                     /locus_tag="SCJ86_002523"
     CDS             complement(5146..5517)
                     /locus_tag="SCJ86_002523"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329142.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YxeA family protein"
                     /protein_id="HEG1170033.1"
                     /translation="MKVIKYLIGLAAISGILLFGLKYYTEGSYGEIPGILDQLNPLVE
                     KGEVYVKTQKPEEVNEYGTARYVQKAADANGKERTVEFNGLSVLKENHYLKISNKGAH
                     IETYEEVPREKVPEKALAIIG"
     gene            5732..5917
                     /locus_tag="SCJ86_002524"
     CDS             5732..5917
                     /locus_tag="SCJ86_002524"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEG1170034.1"
                     /translation="MNQEELFQKVKEMIKNGNFDGAKRFIEEHKEQLGPYKEKAQNLL
                     KDVNIDSVKNKFKNLFK"
     gene            complement(6000..6188)
                     /locus_tag="SCJ86_002525"
     CDS             complement(6000..6188)
                     /locus_tag="SCJ86_002525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344185.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEG1170035.1"
                     /translation="MNPYEQLIQDLIDGKIEKIDVKREELMAFREAWLKLENRKYVVG
                     KASLNGNVTYHYDPTRLF"
     gene            complement(6212..6475)
                     /locus_tag="SCJ86_002526"
     CDS             complement(6212..6475)
                     /locus_tag="SCJ86_002526"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292935.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEG1170036.1"
                     /translation="MDHVVLEIDLHLINNIRVIYYVVSNSVEQRVLTNKINGILAKKD
                     VHRFNNGEGSYYSIPVEKIIYTTVKVREDLDTKKAYEPIFTTY"
     gene            6633..6890
                     /locus_tag="SCJ86_002527"
     CDS             6633..6890
                     /locus_tag="SCJ86_002527"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321369.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucose uptake protein"
                     /protein_id="HEG1170037.1"
                     /translation="MIAIGQFVFYIPFFIMISILFYYIKWTKKKFSVLLASLPAVYFT
                     YQIFSFRHWETTSVLITHIIELTLSVIFLIIWIYFLYKNQN"
     gene            complement(6961..7152)
                     /locus_tag="SCJ86_002528"
     CDS             complement(6961..7152)
                     /locus_tag="SCJ86_002528"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEG1170038.1"
                     /translation="MEAGKVLQEVKALCADKKYEEAKSLIEKNKENLEEKYPIAQEFI
                     ELKQGTILHRFKHFFGITE"
     gene            complement(7293..7613)
                     /locus_tag="SCJ86_002529"
     CDS             complement(7293..7613)
                     /locus_tag="SCJ86_002529"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331812.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEG1170039.1"
                     /translation="MVRPQEVRAPKEKIEILAIIEDGTQTKKGYSIALVKWKGKKGVA
                     LRWDGDNQQDKGFPVTANGYHPAWFVLPDKFMELYSYDYARAAASIKALDKLTEEQNE
                     MEEK"
     gene            complement(7636..8070)
                     /locus_tag="SCJ86_002530"
     CDS             complement(7636..8070)
                     /locus_tag="SCJ86_002530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317690.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein"
                     /protein_id="HEG1170040.1"
                     /translation="MSKRYQNIMVAVDGSRQSIKAFAEALDLAKDNEAHLFIVSIINK
                     VELTHSAYAFSKIYADEKQRTEVAMLKKINDAKEYGIAEIHAIVETGDPRNLIANVIP
                     GQENIDLIVMGATGKGAIQQALVGSTASYVVSHAPCSVLVVK"
     gene            8405..>8586
                     /locus_tag="SCJ86_002531"
     CDS             8405..>8586
                     /locus_tag="SCJ86_002531"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377182.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysM peptidoglycan-binding domain-containing
                     protein"
                     /protein_id="HEG1170041.1"
                     /translation="MTSLKTLLFGTTLAAGAAFFMGTTAHADEAYTVQSGDTLSTISQ
                     KYVGDNSLINAIAESNS"
BASE COUNT         2718 a         1694 c         1362 g         2812 t
ORIGIN      
        1 aggaactgct gcaaataagc aattcctgtt tttatgttca ttgattaatg aatagagccc
       61 attagttttt tgattggttc tttcacagta aatagcaaga taccaccgac gattgctact
      121 gcaccgataa taccgaagta agcagattct gttcccggtg tgtagaagcg tgcaatttgt
      181 gcattgattg cttgtgaaga agcatcagct aagaaccaaa tagcgattgt ttgtgattca
      241 aatgctcgtg gtgctaattt tgttgtcaca gacaaaccaa ctggtgaaag acacaattca
      301 gcaataacca cgatgaagaa acttcccaac aaccacaatg gacttacttt tccacttgta
      361 ccataaagca tacccggcaa catcaatagc aagtacgata atcctgcaaa taaaagtcca
      421 agagagaatt tcactactgt agatggttga cgttcaccaa gtttcgtcca catcgttacg
      481 aatactggtg tcagaataaa gatgaaaatt ggattcaatg attgatacca gcttgcttgg
      541 atcgttacac caaatagcgt agattgtgta cgttcactag caaataaagc taagatagat
      601 gaaccttgtt cttgtaatga ccagaaaatg attgctgcaa ggaataatgg gatataagcc
      661 catactcttg gtttttcttc agcagttaca tcttttgaag tcagcatttt gataaagtaa
      721 tacaacggta agataatccc caatacactg attgtattga caaaaaagtc aattgtcaga
      781 tgaccagtta gttgtgcccc gccgaatact gcagcgataa caacaagtgc gatgatcaag
      841 ttacggatga atcctttacg ttcttcagtt gtcaaaggat tcggcggtgt tgtacccact
      901 ccgtctaatg tcttgcgtcc ttggatgatg tattgtaata atccaaagaa catcccaata
      961 gcagctaaag agaaccctaa atgatagtta tattcttgac cgattgtacc aacgattagt
     1021 ggtgctaata atgcccctaa gttgacaccc atgtaaaaaa tcgaaaatcc tgcatctctt
     1081 cgcaagtcag tctttgaata aagatgtccg accatgccag aaacatttgg tttcaaaaag
     1141 gcggttccga taataatcaa tgccatcgaa acaaacaatg tacttacacc aaatggtaaa
     1201 gccaagacga tgtggccaag cataatgaaa actccaccga agaatacagt gcgttttgct
     1261 cctaacactc ggtcagatac ccatccgccg acgatactag acatgtagat caaagatccg
     1321 tagatcgaca tgatagctaa tgctgttgct cgtggtaatc caagtccccc gttggcaact
     1381 gtatcgtaca tataatagat aagtaatgca cgcataccgt aataactaaa tcgttcccac
     1441 atctcagtga agaacaaagt tgacaatccc ttcggctgcc cgaagaagcc cttgtcttgc
     1501 tgtttttcca taatctatac cccaattctt taaattttca gaaaatatta tacatcttta
     1561 ccgaaaaatt taactaaaaa acttatttaa gaaatataaa ctaattttct ataaaagcaa
     1621 gtaattttta atggcaatat cataaaaaaa agaagataga gatttaataa ttatatatca
     1681 gctgtttttt tcaaataagc aaaaattcct aaaacacaac aatgaaagcg ataactaaga
     1741 aaatccttac ataacgattt ttcttattaa aattgttttt tgaacttcgc ttattttttg
     1801 tgagcatatc tgtttactta ctttttgtaa ggtgctatac ttcatttcgt aatcaattaa
     1861 aaaaatgcaa aacgcaaatc aaaggagaaa ttttattatg acaactttta ctgacacttt
     1921 gaaaaaccgc cgttcaatct atcatctagg acgtaatgtt tcactttcta atgaagaatt
     1981 aacaactttg atcaaagaag caattaaaga aagcccaact gctttcaatg ctcaatcaac
     2041 acgcgcagtt atccttttcg gagatgcaca tgaaaaactt tgggaaatca ctgaagaagc
     2101 acttcgccca ttgactccag cagaagcttt cccaaataca caaaacaaat tagctggttt
     2161 caaaaatggt tatggtacag tattattctt caaagatact gacgtggtaa aaggtctaca
     2221 agaacaattt gaattatatg cagacaactt cccagattgg tcagaacaat caaacggaat
     2281 cgcaacagct aatacttggg tagcattagt tgataaagga ttgggcgcaa acttgcaaca
     2341 ttacaaccca gttattgacg aagcagttgc aaaagaatgg aatatccctt caaactggaa
     2401 actacgttcg caattagtat tcggttcacc agaaacaccg gctggggaaa aagaatacat
     2461 gaacgatgcc gatcgcttcc gcgtatttgg ataattttat ttgaaataaa aaacagaggt
     2521 tgtgacaaac atcttgtcgc aacctctgtt tttattctgg atcaatcata atatcaatct
     2581 ctgcctcatc gtttaagcgt tgggacaaaa atggaactaa ttcttctaat gatgcagaaa
     2641 atttcagcat cggtcctcgc catccgtttc ggaaaaaatt catttttacg attttagaac
     2701 cttttggctg atataataca attttattga tctcagaaaa aggaacacct tttttgtcaa
     2761 agctattcaa aacaagtcct tcttctgtga ttcctctact gtccaacaaa aaactaataa
     2821 aaatcattgc tgacaagatc ccttttgcca attcatctgc tgtaggatga ctgatgagtg
     2881 tataccaaat gactagtgac actaagaagg gtatcaccaa gtagacccat cgatttcgca
     2941 cagtccgata aataatttct tttcgaacca gccaaagaaa taaaaacatc acaacaacga
     3001 ataaaaacaa tagaaccgta aagaatgtta ccatgacaac tcgcctcgct ttttcttctc
     3061 cagaaaatag gaaaagaata ctgatttacc ttctcaaaga aattggactt gcagcacgcc
     3121 gcacgtccaa ttgttttctt tattcaaatg tatcatattt tgcaacaacc atgcttgctt
     3181 tttcatctaa taattcttgg tcctctgcag cacattgggc aactttgacc acggcgcagt
     3241 ttgtcatttt tgatacaact tcaccaatga ccgtttttgt aaaaccaatt ggtttgcatg
     3301 catattgttc tcctaaatta atcatttctt ccgtcatcaa aatcctccta aactgctgga
     3361 acttttctcc acattaccac tgctatttct ttggagattt aactcattga tttttattta
     3421 cagttaaaaa tcaaccttac aggcattata gcgtactgtt ttcgttttca caattaaaac
     3481 agctgttgta tttagaagaa tttttttcaa aaaattaaaa catattcctt aaaaactaat
     3541 tatttcctac atattaaaat ataatacttc catttccaat aaaaatacat ttataatagg
     3601 aaagataaac agctgaaaag ggagatttca aatgaagaaa aaaacgttca aaaaactatt
     3661 gtttctaggg ggcacagctg ctcttttatt aagcggtaca agttattacc tacatatcca
     3721 ttctccagaa catacaatga acactgcaca aaaaagttca acacaaaaaa cgaaaaagac
     3781 aaaagagaaa gcagaagttt tactagatgt tccattagag agccagtttg attctccttc
     3841 tttagaaaat ggctgtgaag tcacttcgct ttctatgctt ttatcttttt atggttatga
     3901 aacaactaaa aaccagttag ctgagcaatt ggattacgtc cctgttttca atgcagatgg
     3961 gactcatggc gatcctaatg aaggatttgt tggtgatatc agtggcggag actgggctat
     4021 ggtcgtctat gttcccccag ttgcctccct tgctcagcaa atcgtccaaa cggattatca
     4081 cacttttcca aaaaccgatg ctcaactcga tgatatcaaa aaagcactat ctaaaggaaa
     4141 tcctgtctgg acttctgtga ctatcaattt tgaagtccca gatgaaactg attttatgac
     4201 ttggaccaca aataatggag aagtaagagt cactcctctt gttcatgctt gtgtagtcac
     4261 tggatatgac aaagaaaata tttatgtcaa cgatccctat ggtgtaaaaa atcgagcagt
     4321 accgatcaac gatttttcgg cgattttcac tgcaatgggt ggacagatgc taactttgga
     4381 gaaaagctag taattcaaac aaaaatccct tatactcaag ataccaaacc tttgaaggga
     4441 gcgtagataa tggaaaaatt cacacataaa aaaatggatc caaatgaaat tccgattatt
     4501 tttgtccgtg actgcaaggg aaatgttcaa ggtaaagtct ctattaatga gtggaacgag
     4561 cgtcgcagac ctgctaccct caatgaactg gaaatcaaat tgtatcgtca atctcttgtt
     4621 tattacgcag atcaagagta cgagaaagcc accgatttat tgaaatttct aattgctcga
     4681 acagagtata ctcgctttga atatattgaa cgtttagcaa acatctatca tattatgaat
     4741 gaacctgtca aagaatatca gttattggac accgtcttgt cagtagcaga actgattgcc
     4801 cttcctgctg gattagaaaa gaaactagta cgccgccttt tacgagtaaa gcaacaacta
     4861 tccgatcaag aaaaataact tgcgtttttt taggaaagtg ctatgattat tttgttgaat
     4921 aaatgggatg tagccaaact ggtaaggcac ttgactctga atcaagcaat tgtaggttcg
     4981 aaccctgcca tcccaatttg atgtgcttgc tgaaaaaatg aaccccctgt aaaagaacaa
     5041 tctttttgca gggggattat tgtattattt ttactacttg tatttagttt acataattaa
     5101 attatgaata ataaaatcgg gcgtattctt aatacaaaaa ctgatttacc caataattgc
     5161 taatgctttc tccggaactt tttctctagg aacttcctca tacgtttcta tgtgtgctcc
     5221 tttattgcta attttcaaat aatgattttc ttttaacaca gaaagaccat taaattcaac
     5281 cgttcgttct ttcccattgg catcagcagc tttctgcaca taccttgctg taccatattc
     5341 atttacttcc tctggttttt gtgtctttac atagacctct cctttttcaa ctaatggatt
     5401 tagctgatca agtataccag ggatttctcc ataacttcct tcagtataat atttcagtcc
     5461 aaatagcaaa ataccactaa tcgccgccag tccaatcaaa tatttaatca ctttcatctt
     5521 tctacctcct tgttatatat tcagtatatg ggtcagttaa agaaattcca tccgtcccaa
     5581 gttggagatt tttctcctac tttagaagcg agaatcgagt gtttttttag taaaaatgat
     5641 cgttatttga tattattgac gtgtggataa atcagttttt ctcgttttgt ccatgtaaaa
     5701 aatcatgttg atagcaaagg atgtgttcaa aatgaatcaa gaagaattat ttcaaaaagt
     5761 caaggaaatg attaagaacg gcaatttcga tggcgcaaaa cgatttatcg aagaacataa
     5821 agaacaatta ggaccttata aagagaaagc ccaaaatctt ttaaaagatg taaatatcga
     5881 cagtgtcaaa aacaaattta aaaatctatt caaataagta aaactttcac ttttgtaaaa
     5941 tcaaaggagg acaatgctgc tgcatcgtcc tcctttggtt cttttctgaa tacttcatac
     6001 taaaataaac gtgtcggatc gtaatgataa gtcacattgc cgttcagtga ggcttttccc
     6061 accacatatt tacgattctc caacttcagc cacgcttctc gaaaagccat taattcttct
     6121 ctttttacat caatcttttc gatttttcca tcgatcaaat cttgaataag ttgttcatat
     6181 ggattcattt ttttcctcca tctgctgtca atcaataagt tgtaaaaata ggttcatatg
     6241 cttttttggt atctagatct tctctgactt tgactgtcgt ataaataatt ttttctactg
     6301 ggatactata ataacttcct tcaccattat tgaatcggtg aacgtctttc tttgctaaaa
     6361 taccatttat tttattcgtc agtacacgtt gttcgacact gttacttaca acatagtaaa
     6421 tcacccgaat attattgatc aaatgcaaat cgatttctaa cactacatga tccatgaaaa
     6481 tcccactcct tctttcactt tgattatagc aagtttctcc tctaatttca aaatcgatac
     6541 agattttacc ggataaatcc acaaaactgc ttgcgccatt tcgcacaaag tgttattttg
     6601 tatcatggac tttatttatc ggaggcaata gtatgatcgc gatcggtcaa tttgtttttt
     6661 atattccttt ttttattatg ataagcattc ttttctatta cataaaatgg acaaaaaaga
     6721 agttttcagt tttactggcc tctttacctg ctgtatattt tacttatcaa attttttctt
     6781 ttaggcattg ggaaacaacg tctgtattaa taacacacat catcgaattg acgctttcag
     6841 taatttttct tattatttgg atctattttt tatataagaa tcagaattga atccctcgca
     6901 tgtttaaaaa gaaaacgatt aaagatgcag caaatttctg ccgatcttta atcgtttttt
     6961 ctattctgtt ataccaaaaa aatgtttgaa tcggtgaagg atcgttcctt gttttaattc
     7021 tataaattct tgtgcgatcg ggtacttttc ttccaaattt tctttgttct tttcgatcag
     7081 gcttttcgct tcttcatatt ttttatctgc acatagtgct tttacttcct gtaatacttt
     7141 tcctgcttcc atttgcctca ccctcttgcc attttattgc tttaagtttt atttaagtta
     7201 tacgcattat tctagtttca tcccaaataa ccttttttca tttttactcc tcgaactaat
     7261 cagttttcta aaggctgatt ggttcgtctt ttttattttt cctccatttc attctgctct
     7321 tctgttaatt tatctaatgc tttgattgat gctgcggctc ttgcataatc atagctgtat
     7381 aattccataa atttatcagg cagaacaaac cacgctggat gataaccgtt agctgtgaca
     7441 gggaatcctt tatcttgttg gttatctcca tcccatctca gagcaacacc tttttttcct
     7501 ttccacttta ctaaagcgat cgagtatccc ttctttgttt gtgtgccgtc ttcaataatt
     7561 gctaaaattt ctattttttc ttttggcgca cggacttctt gaggacgaac cattttctat
     7621 tcctccctta tctcattact tcacgaccaa cacactgcaa ggggcatgtg atacgacata
     7681 agaagcggtc gatccaacta atgcttgttg tatagcacct tttcctgtcg ctcccataac
     7741 gatcaaatca atattttctt gtccagggat gacattggca atcaaatttc ttggatctcc
     7801 agtttcaact attgcgtgga tctcagcaat gccgtactct ttggcatcat tgattttttt
     7861 cagcatggct acttctgttc tttgtttttc atctgcatag atttttgaaa aggcataggc
     7921 actatgggtc agctcaactt tgttgatgat tgacacgata aataagtggg cttcattgtc
     7981 ctttgctaag tcaagggctt cagcgaacgc tttgatcgat tgtcttgatc cgtcaactgc
     8041 taccatgatg ttctggtatc ttttactcat tggaaccgcc tcctttttct tttattgtaa
     8101 catctcaagc aaaaatacaa aaaatatacg caatatgtaa ataaaatgat gaacaaaagc
     8161 aatttctttc aagttattta tacatttctt ttctcatcag aacacctgtt ctaatcccat
     8221 tatttctgca ttatgtgaaa ttcctcatca tttttcacat cgagcgaaca tcattctgtt
     8281 acaaaaaacg tggatttgtc ataaattgtc atgcttcttt tacctatgaa atattttcac
     8341 gtgttaaagt attcctcgtt gaacacaaca aataaaactt tttaacgaat aaaggagatt
     8401 tttcatgaca tcacttaaaa ctttactttt tggaacaact ttggctgccg gcgctgcatt
     8461 cttcatggga acgactgctc atgcggacga agcttatacc gttcaatcag gtgatactct
     8521 atcaacgatt tctcaaaaat acgttggtga taactccttg atcaatgcca ttgcagaatc
     8581 aaattc
//
LOCUS       DARBET010000097         8494 bp    DNA     linear   BCT 07-NOV-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4027
            SAMEA114569841-rid20112183.denovo.099, whole genome shotgun
            sequence.
ACCESSION   DARBET010000097 DARBET010000000
VERSION     DARBET010000097.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA114569841
            Sequence Read Archive: ERR12203666
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 8494)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8494)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001972618.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001972618.1
            Genome Coverage       :: 132x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/07/2023 13:18:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,041
            CDSs (total)                      :: 2,975
            Genes (coding)                    :: 2,908
            CDSs (with protein)               :: 2,908
            Genes (RNA)                       :: 66
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 67
            CDSs (without protein)            :: 67
            Pseudo Genes (ambiguous residues) :: 0 of 67
            Pseudo Genes (frameshifted)       :: 26 of 67
            Pseudo Genes (incomplete)         :: 28 of 67
            Pseudo Genes (internal stop)      :: 20 of 67
            Pseudo Genes (multiple problems)  :: 6 of 67
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8494
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA114569841-rid20112183.denovo.099"
                     /isolate="VRE4027"
                     /db_xref="taxon:1352"
                     /country="Denmark"
     gene            complement(<1..173)
                     /locus_tag="SCJ86_002532"
     CDS             complement(<1..173)
                     /locus_tag="SCJ86_002532"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006735101.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEG1170042.1"
                     /translation="MATKIADELENHVLETGMQKITTHASITAKPFFEKRGYKVINEQ
                     TVELRGQLFTNFL"
     gene            complement(593..1714)
                     /locus_tag="SCJ86_002533"
     CDS             complement(593..1714)
                     /locus_tag="SCJ86_002533"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010730282.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS4 family transposase"
                     /protein_id="HEG1170043.1"
                     /translation="MDKYNVKTSFQKWFSAINFSGLSENSQSLISDFDFYSKKLDFQT
                     TLKVLLHAVYEELPSYREIDRAFLDQRLCKELGIDSLCYSSLSRRAPEIKQEVLMEIF
                     TQLVARISAQQPSSKTTSLQLIDSTTIPLNKAWFPWAKFRKTKSGIKLHLNLCYLDKT
                     NQYPESFTMTNASEHDRNHLEVLVDKTQATYVVDRGYFDYKLLDKLNRDGYFFVTRTK
                     SNTKITILDQIEVADTTTRDGTIISDQQVILVGGVNHVTERFRLVTVLTKGQKILRMV
                     TNLFDVSPNEVADMYQARWQIELLFKHLKQNLTIKRLYSHSEQGAINQVILTLIATLL
                     TYVIKIELNTTATLFQLKRTFHYLMFEPAEEWFKRYRPG"
     gene            2042..3451
                     /gene="istA"
                     /locus_tag="SCJ86_002534"
     CDS             2042..3451
                     /gene="istA"
                     /locus_tag="SCJ86_002534"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS21 family transposase"
                     /protein_id="HEG1170044.1"
                     /translation="MDDIKTIKHLRNNRDQSIDQIRKAMNINWRTAKKYADSDQLFIT
                     DPTRKNKSMMDDGWGEIIGSWLEEDLRLRRKLRRTAKAIHEQLKPLGFPGSYRTVCVY
                     ISQWKKQRYDETNYQAVDRLDHPIGEAQVDFGTMEVMHEDHMKDIQLLIMSFPYSNAT
                     SASAMPAQNQECFLEALKQLFNQCGGVPKTIRIDNLTPAVKKTKSKFEEAQLTDEFLR
                     FTIHYGCDVQVCNPRSGHEKGHVENKVGYVRYNFFSTSPIMDSFESLNKQLEEQLKAD
                     RKRTHYSKHQLIEDLWKKENKALHSLPQEDYPIFKEVEAKLNRYHEMKLDQTVIHIPK
                     AYGYSQLTVVLTWNKFKVLTPHGEILMNEYRPYMNKKRLIPWSDIMKQWSNKLNVVSY
                     SRYWKYLPARLQNYLAIDNNHLKYRRIKQLLSLLATYDIQQINEQFYELVVQEDAPSD
                     FDVNWSNYDALTKTGGVQK"
     gene            3448..4176
                     /gene="istB"
                     /locus_tag="SCJ86_002535"
     CDS             3448..4176
                     /gene="istB"
                     /locus_tag="SCJ86_002535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289053.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS21-like element helper ATPase IstB"
                     /protein_id="HEG1170045.1"
                     /translation="MIGQTLEDYCKKLRLSHIITNIPRASFEAPEQFITDLFRLELET
                     RQQIKLERMEKLAKFPVPKSLKNYEWHEDIFLPSHLDKETLVSLDFLKRQENIVCVGS
                     PGTGKTHLAIALGRKACSEGVSTKFFRVNNLVEQLENALKKQQLASFKKRFETTELII
                     LDEMGYLPLTKEGAELLFQLISDWYEQKSIIITSNLEFSQWNRVFMDSRLTAALVDRL
                     IHHAHIISFKGESFRLKNSLSKSY"
     gene            complement(4304..5215)
                     /locus_tag="SCJ86_002536"
     CDS             complement(4304..5215)
                     /locus_tag="SCJ86_002536"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297345.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein"
                     /protein_id="HEG1170046.1"
                     /translation="MKIKIERSNKEKIPKKKKERVVSVGKHRKMVLALWLLLSCSLAF
                     GIYKNFTAIDQHTTHEKVVVKEKIVNTSGVENFTVDFVKEYFSWKNNKEVIEKRMTNL
                     EQYLTEEGLALSQDMVRADIPTSSEVVSVKILDVEKHSEEFVVSFVVDQKITEGKKTQ
                     SISSAYRLMIFEDEKGNHIVTSLPTMIGKPEKAKHKVRQVKSDSKIDSKTTEEITEFL
                     ETFFRLYPAASEKELEHYVEKDAMQPINTNLKFVELVNPIYKQKGKAIQVIVGVEYLD
                     ELSKTTSVFQYRLKLYKNENWTISNCE"
     gene            complement(5232..6239)
                     /locus_tag="SCJ86_002537"
     CDS             complement(5232..6239)
                     /locus_tag="SCJ86_002537"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289057.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein"
                     /protein_id="HEG1170047.1"
                     /translation="MNLKKLSIISLTFLTISFVGILMCVGLLFGEDGEGSGSSGTSPG
                     GSSVSEVVLKHRSMVEKYAKESDITEYVSILLAIIQVESGGTMEDVMQSSESAGLSPN
                     SLSTEASIKQVCLYFSSLVKCSKELGCDQDSIIQAYNYGDGYLDYVAKNGKKHSFALA
                     ESFSKEKSGGQKVDYPNPIAIKENSGWRYNYGNMFYCLLVKQYLTTTQFDDKTVQGIF
                     DEAFKYEGTAYVFGGSSPGTGFDCSGLTQWCYAKVGLKLPRVAQDQYDAMTHIDLKDA
                     KPGDLIFFHSTYDAGTYVTHVGIYAGENRMFHAGDPVGWTNLTDSYWQQHIIGAGRYK
                     Q"
     gene            complement(6236..8365)
                     /locus_tag="SCJ86_002538"
     CDS             complement(6236..8365)
                     /locus_tag="SCJ86_002538"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341749.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FUSC family protein"
                     /protein_id="HEG1170048.1"
                     /translation="MKKKLFRIVGIVFISIVTLTVLLSLVGTVAEATGLVDDTVEAGN
                     LYSKYSLNNYQLDFFVDSSWDWLPWNWGDGLGKSVMYGLYAITNFIWTVSLYLSNATG
                     YVVQEAYKLDFISDTAKSIGKNIQTLAGITENGLQTSGFYFGFLLLMILALGVYVAYT
                     GLLKRETTKAVRTVINFVMIFLLSGSFIAYAPTYITKINDFSSDVSEAALSLGTKIVV
                     PNSESQGKDSVDLIRDSLFSIQVQQPWLLLQFDDSNIEEIGEGRVNKILSVSPDENKG
                     KDREEAVKAEIEDNDNANLSITKTMNRLGTVVFLVLFNIGISFFVFLLTGIMLFSQIL
                     FIIFAMFLPISFLLSMLPTYESLGKRAIIRLFNTIMMRAGVTLVITTAFSISTMFFNI
                     SATYPFFMVAFLQIVTFAGIYFKLGDIMSMFNLQSNDSQSMGRRVMRKPQMLMNRKLR
                     QLNRNVGRTFAFGGGAAVGGKLATSSTKGKGSSKINRVSNRKNHSARSINELNSDLKK
                     QEQPVGTKKESRINLVGRKVGKVLDTKEIVKDKAKQAKNQVTDAPTNLKYSLHKGMEK
                     TKKVPEDFKRGPFEEKANRGELREKQRQRRDAKMDEKRKVLNEAWNRHEKGRENVPIK
                     KEIKPQKNELPENRSLKPRVQVHSNPEIKRKLSSQEVIPNGSTQPLSKNNSFQQVKQR
                     FTFPKRTNQQVQRKKNTRPKPKPGEKR"
     gene            complement(8370..>8494)
                     /locus_tag="SCJ86_002539"
     CDS             complement(8370..>8494)
                     /locus_tag="SCJ86_002539"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301706.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HEG1170049.1"
                     /translation="NGQCLMQDLYGRVGVVQVHPVFEELFHAFDTRPPVQERME"
BASE COUNT         2599 a         1728 c         1486 g         2681 t
ORIGIN      
        1 attaaaaagt tagtaaataa ttgccctcta agttccacgg tctgttcatt aataacttta
       61 tatcctcgtt tttcaaaaaa tggctttgcg gtaatggaag cgtgggtagt gattttttgc
      121 ataccagttt ctaaaacatg gttttccaat tcatctgcaa ttttagtcgc aatacctttt
      181 ccttgatggc tatgtttttg tcacaacttc agtattcctg taacaatacg gatttcagta
      241 tacaacaaca gcttttgaaa agatacttgc aaaaggatta agatatctat gacctgctat
      301 gctcactgat gttgaaaaga atgttttatt aggattgagg taagcaagat agtgaatatg
      361 tccggaaagc ctgtaaaata aggcttttcg gacatatgtg aagttataaa aagatagcga
      421 aaaatctatt tagtatcact aatgttgcga acatatcgtc agaatagtct gacatttatt
      481 tcttgctaaa aatttcaaaa aagtcaagtg ctgacgcagc ggatgactat tttccagaaa
      541 attttagtat gtattcaaaa atcaaacgtg acaacagcta ttaactacat cctcatccag
      601 gtctgtaacg tttgaaccac tcttctgcgg gctcaaacat taagtaatgg aaggtacgtt
      661 tcaattgaaa cagcgtcgct gtcgtattca actcaatttt tatcacataa gtgagaagcg
      721 ttgcgattaa ggtgaggatg acttgattta tcgccccttg ttcactatgt gaatatagtc
      781 gtttaatggt cagattttgt tttaagtgtt tgaacaacaa ttcaatctgc caacgggctt
      841 gatacatgtc ggccacttcg tttggcgata catcaaagag attggtcacc attcgcagga
      901 ttttttgtcc cttggtaaga accgttacca acctaaaacg ctctgtcaca tggttgacac
      961 cgccgacaag aatcacttgt tggtcactga ttatcgtccc atcacgggtt gttgtatctg
     1021 ctacttcaat ttgatccaag atggtaattt ttgtattaga ttttgtccgt gtaacgaaga
     1081 aatagccgtc acgatttagt ttgtctaaca acttgtaatc aaagtagccg cgatccacca
     1141 catacgttgc ttgcgtttta tcaacaagaa cttcaaggtg attacggtca tgctcactcg
     1201 catttgtcat cgtaaaactt tcaggatact gattggtttt atcaagataa cagagattca
     1261 aatgtagctt gatgccagac tttgttttcc gaaacttggc ccatggaaac catgctttgt
     1321 tcaacggaat agtcgttgaa tcaatcaatt gtagactggt tgtttttgag cttggctgct
     1381 gagcagaaat tctggcgact aattgcgtaa aaatttccat caatacttct tgcttgattt
     1441 ctggcgctct acgagataac gagctgtaac acagggaatc aatgccaagt tccttgcaga
     1501 ggcgctgatc taaaaaggcg cgatcaatct cgcgatagct tggcaattct tcataaaccg
     1561 catgaagtaa cactttaagc gtcgtttgga agtctagttt tttactgtag aaatcaaagt
     1621 cgctaattaa gctttgagag ttttctgata aaccagaaaa atttattgca gaaaaccatt
     1681 tttgaaatga ggtttttacg ttatacttat ccatgcgtga gtccttttta ttggtcttgg
     1741 ataagtcatc cgagaatagt ataaaggact tttttgcgtt tggatagctt tgtattaggc
     1801 ttttgatggg ttaaaaagtc tttgcaacac tagtaattta gtatttatgt ataaattgaa
     1861 agatatttta ccaccactta gtcttcttaa aagtgatcca acattttgtt tgtggcagaa
     1921 tttaactaca cactaatgca gccgaaagta cacaactatg cagcagtttg actgcgaact
     1981 ggtttccttc catcataagt ttgaatgact tagtgatgga ggtacagaaa ggttgttaac
     2041 tttggatgat attaaaacaa taaaacattt gagaaataat cgtgatcagt caattgatca
     2101 gataagaaaa gcaatgaata tcaattggcg aacagcgaaa aaatacgccg atagtgatca
     2161 actattcatt acagatccca ctcgaaaaaa caaatccatg atggatgatg gttggggaga
     2221 aatcatcggt agttggctag aagaagacct acgtttacgc agaaaacttc gtagaacggc
     2281 caaagctatc catgaacaat tgaaaccttt gggtttccct ggctcctatc gaacagtttg
     2341 tgtatacatt tctcaatgga aaaagcagcg ttacgacgaa acgaattacc aagctgtcga
     2401 tcgtcttgat catccgatcg gagaagcaca ggtcgatttt ggaacaatgg aagttatgca
     2461 tgaagatcac atgaaagata tacaacttct tatcatgtca tttccctata gtaatgcgac
     2521 gtccgcaagt gccatgccag ctcaaaacca agagtgtttc ctggaagcat taaaacaact
     2581 atttaatcaa tgcggcggcg ttccaaaaac gatcagaata gataacttaa ctccagcggt
     2641 gaaaaaaaca aaatcaaaat ttgaagaagc acaactaacc gatgaattct tacgtttcac
     2701 cattcattat ggttgtgatg ttcaagtctg taacccacgt agtgggcatg aaaaaggaca
     2761 tgtcgaaaat aaagtgggtt atgtccgata caatttcttc tcgacttcgc caatcatgga
     2821 ctcttttgag tccctaaata agcaacttga agaacaatta aaagcagatc gaaaaaggac
     2881 gcattattca aaacatcaat taatcgaaga tttgtggaag aaagaaaata aagcgttaca
     2941 ttctcttcca caagaggatt acccaatttt taaagaagtg gaagctaaac taaatcgtta
     3001 tcacgaaatg aaattggatc aaacggttat tcatattcct aaagcatatg gctattctca
     3061 attgactgtt gttttgactt ggaataaatt caaagttctt acgcctcatg gagaaatatt
     3121 aatgaacgaa tatcgcccct atatgaacaa aaaaagattg atcccatgga gtgacatcat
     3181 gaaacaatgg agtaataaat tgaatgtcgt tagttattcg aggtattgga agtacttgcc
     3241 tgcacgcctt caaaattatc tagcgattga taataatcac ctgaaataca gacggataaa
     3301 acaattacta agtttattag cgacctacga tatccaacaa attaatgaac aattttacga
     3361 attagtcgtt caagaagacg ccccctctga ctttgatgtg aattggtcaa actatgatgc
     3421 attaacaaaa acaggaggtg tgcaaaaatg attggacaga cgttagaaga ttattgtaaa
     3481 aagcttcgat taagccatat catcacgaac ataccaagag cttctttcga agctccagaa
     3541 caatttatta ccgatttatt ccgtttagaa ctggagacca gacagcagat caaattagaa
     3601 cggatggaaa aattagccaa atttcctgta ccaaaatcgc taaagaacta tgaatggcac
     3661 gaagatattt ttctaccatc tcatctagat aaggagaccc ttgtctctct tgattttcta
     3721 aaaaggcaag aaaacattgt ttgtgttggc tctcctggta cagggaaaac acatctagcg
     3781 atcgcattag ggagaaaagc ctgtagtgaa ggtgtttcaa ccaaattttt ccgagttaac
     3841 aatctcgtag agcaattaga gaatgcatta aaaaaacaac agctagccag tttcaagaag
     3901 cgattcgaaa caacagaatt aattattctt gatgaaatgg ggtacttacc attgacaaaa
     3961 gaaggagctg aacttctctt ccaactgatt tcagattggt atgagcaaaa gagcattatt
     4021 attacctcta atttagaatt cagtcaatgg aatcgagtgt ttatggattc tcgattaaca
     4081 gctgctctcg ttgatcggct aatccatcac gctcacatta tcagcttcaa aggcgaaagt
     4141 ttcagattga aaaattcatt atctaaatca tattgaagga aaaaactagt tgcagcaaaa
     4201 ctctgcactt tcggctgcat ttttttgtaa aaagctattg cacaacacaa acatttgaac
     4261 gacgtaattc caaatgttgg attgctttta gataatcttt tatttattca caattgctaa
     4321 tagtccaatt ttcatttttg tatagtttta atctatactg aaaaacactt gttgttttgg
     4381 acaactcatc aagatactca acaccaacaa tcacttgaat cgctttaccc ttttgtttgt
     4441 aaataggatt cactaattcc acaaatttca aattagtatt aattggttgc atcgcgtctt
     4501 tttcaacata atgctctagt tctttttctg aggctgctgg ataaagcctg aaaaacgtct
     4561 ctaaaaactc ggtgatttct tccgttgtct ttgaatcaat ttttgaatca ctttttactt
     4621 gtcttacttt atgttttgcc ttttctggct taccaatcat agtgggtaaa ctcgttacaa
     4681 tatgattgcc tttttcatct tcaaagatca tcagacgata tgcagaagaa attgattgtg
     4741 tctttttccc ttctgtaatc ttttgatcta ccacaaatga aacgacaaat tcttccgagt
     4801 gtttttcaac atctagaatt tttactgata caacctcgga actagtggga atatccgctc
     4861 gaaccatatc ttggctcaaa gcgagccctt cttctgtaag gtactgctca agattagtca
     4921 ttctcttttc aattacctcc ttgttattct tccatgaaaa atattctttc acaaaatcta
     4981 cagtaaaatt ttctactcca gaggtattca ctattttctc tttcacgacc actttctcat
     5041 gagttgtatg ctgatcaatg gccgtgaagt ttttgtagat tccaaaagca aggctacaag
     5101 acaataaaag ccaaagggca agcaccattt ttcgatgctt gcccacggat actactcgtt
     5161 ctttcttctt ttttggaatc ttttccttgt tacttcgctc aatttttatc ttcattttcc
     5221 attcctcttt tctattgttt ataccgacca gcaccaataa tgtgctgttg ccaatagcta
     5281 tcggtaagat tggtccaacc caccggatct cctgcatgaa acattctgtt ctctcctgca
     5341 taaatgccca catgagtcac ataagtaccg gcatcataag ttgaatgaaa gaaaatcaaa
     5401 tcgcctggtt tggcatcttt taagtcaata tgagtcatag catcatattg atcctgagcc
     5461 acccgaggga gtttcaaacc aactttggca taacaccatt gagttaaacc actacaatca
     5521 aacccagttc cgggacttga cccaccaaat acataagccg ttccttcata cttgaacgcc
     5581 tcgtcaaaaa ttccttggac tgttttatca tcaaactgtg ttgtagttag gtactgcttc
     5641 actaataagc aataaaacat attgccatag ttataccgcc acccactatt ttccttaatc
     5701 gcgatgggat tgggataatc gactttttga ccacccgatt tttcttttga aaaagattcg
     5761 gctaaagcaa aactatgctt ctttccgttt ttcgcgacat aatccaaata cccgtcacca
     5821 tagttataag cctgaatgat actatcttga tcgcatccta gttctttgga acacttaacc
     5881 aaagacgaaa aatagagaca cacttgtttg atggatgcct cggtacttaa tgaattcggt
     5941 gataaacctg ctgattcaga actttgcatg acatcttcca ttgtacctcc agattcgact
     6001 tgaataatcg ccaaaagaat cgaaacatac tctgttatat ctgattcttt ggcatacttt
     6061 tccaccattg aacggtgttt caaaactact tctgagacac tactacctcc aggagaagtt
     6121 ccagaactac cacttccctc accgtcctca ccaaacaaca aacccacaca catcaagatg
     6181 cccacaaaag agatggtcag gaaggttaac gaaataatac tcaatttttt taagttcatc
     6241 ttttctcacc tggctttggt tttgggcgag tgttcttttt tctttgtact tgttgattgg
     6301 ttcgctttgg aaaagtgaat ctctgtttca cttgttgaaa ggaattgttc ttgcttagtg
     6361 gctgagtgct tccgtttgga atgacttctt gactagaaag tttccgtttg atttcaggat
     6421 tcgaatgtac ttgtaccctt ggttttaaag acctattttc tggtagttca tttttctgtg
     6481 gttttatctc cttttttatc ggaacatttt cccttccttt ctcatgacga ttccatgctt
     6541 cgttcaatac tttgcgtttt tcatccattt ttgcgtctct tcgttgtcgt tgtttttctc
     6601 gcaactcccc tcgatttgct ttttcttcga aaggccctcg cttaaagtcc tctggtactt
     6661 ttttcgtctt ttccatgcct ttatgaaggc tgtatttcaa gttagttggt gcatcggtta
     6721 cttgattttt tgcttgtttc gctttgtcct tcacgatttc ttttgtatcg agtacttttc
     6781 caacttttct accaactaaa tttattcgtg attctttctt cgtgcccact ggttgttctt
     6841 gttttttcag atcagaattt agctcgttaa tgcttctcgc ggaatggttc tttctattgg
     6901 acactctatt gatttttgaa ctacctttcc cttttgtaga actggtagct agtttcccac
     6961 caactgctgc accgccacca aaagcaaaag ttcgacccac atttcgatta agctgtcgca
     7021 attttcgatt catcaacatt tgcggtttcc gcatgactcg ccttcccatt gactgactat
     7081 cattactttg aaggttaaac atgctcataa tgtcacctaa tttgaaatag ataccggcaa
     7141 aggtcacaat ttgtagaaaa gctaccataa agaagggata ggtggccgaa atattgaaaa
     7201 acatcgtgga gatactgaat gcagtcgtaa tgactaaggt aactcccgct cgcatcataa
     7261 tcgtattgaa caaacgaata atcgcccttt tgcccaaact ctcatacgtc ggcaacatcg
     7321 acaataagaa actaatgggc agaaacatcg caaagataat gaaaaggatt tgtgagaaca
     7381 acataatccc tgtcagaaga aacacaaaaa aggaaatccc aatattaaac aacactaaaa
     7441 agaccacggt tcctaaacgg ttcatggtct tggtaatact gagattcgca ttgtcattat
     7501 cttcaatctc tgctttgact gcttcttccc gatccttacc cttattttca tccggactca
     7561 cggataaaat cttattaacc cgaccctcgc caatttcttc tatattggaa tcatcgaatt
     7621 gtaatagtaa ccagggttgt tgaacttgaa tcgaaaataa actgtctcga atcaaatcca
     7681 cactatcttt tccctggctt tctgaattag gtacaacaat cttggttcca aggctaagag
     7741 ccgcttcact aacgtctgag ctaaagtcat tgatttttgt aatataggtg ggcgcatacg
     7801 caatgaaaga acccgagagt agaaaaatca tcacaaaatt aatgacggtt cggactgctt
     7861 tggtagtttc tcgttttaat aatccggtat acgccacata aactcctaga gctaaaatca
     7921 tcaataatag gaacccaaag tagaatcccg aagtctgtaa tccattttca gtgataccgg
     7981 ctaaagtttg gatattcttt ccaatacttt tagccgtatc cgaaatgaaa tccaacttgt
     8041 aagcttcttg aaccacatag cccgtggcgt ttgataaata cagacttact gtccaaataa
     8101 aattggtaat ggcataaaga ccatacatca cgcttttccc taagccatcc ccccaattcc
     8161 aaggtaacca atcccaagaa ctatccacaa agaaatccag ttgataattg ttcagagaat
     8221 atttggaata aaggtttccg gcttcaactg tatcatcaac cagacctgtc gcctcagcta
     8281 ccgtaccgac taaactcaat agaactgtca gagtgacgat gctaataaaa acgattccca
     8341 caatccgaaa aagtttcttt ttcatcccct cactccattc tctcttgaac cggcggacga
     8401 gtgtcaaaag catggaagag ttcttcaaag actggatgaa cttgaaccac acctactcgc
     8461 ccatacaagt cttgcatcaa gcattgaccg tttt
//
LOCUS       DARBET010000098         8209 bp    DNA     linear   BCT 07-NOV-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4027
            SAMEA114569841-rid20112183.denovo.100, whole genome shotgun
            sequence.
ACCESSION   DARBET010000098 DARBET010000000
VERSION     DARBET010000098.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA114569841
            Sequence Read Archive: ERR12203666
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 8209)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8209)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001972618.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001972618.1
            Genome Coverage       :: 132x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/07/2023 13:18:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,041
            CDSs (total)                      :: 2,975
            Genes (coding)                    :: 2,908
            CDSs (with protein)               :: 2,908
            Genes (RNA)                       :: 66
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 67
            CDSs (without protein)            :: 67
            Pseudo Genes (ambiguous residues) :: 0 of 67
            Pseudo Genes (frameshifted)       :: 26 of 67
            Pseudo Genes (incomplete)         :: 28 of 67
            Pseudo Genes (internal stop)      :: 20 of 67
            Pseudo Genes (multiple problems)  :: 6 of 67
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8209
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA114569841-rid20112183.denovo.100"
                     /isolate="VRE4027"
                     /db_xref="taxon:1352"
                     /country="Denmark"
     gene            476..1339
                     /locus_tag="SCJ86_002540"
     CDS             476..1339
                     /locus_tag="SCJ86_002540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DegV family protein"
                     /protein_id="HEG1170050.1"
                     /translation="MYQIVTDSCCDLPYHVLAKEKVDFLSMNLQLNDQELVDDLGKTF
                     NYDRYMNQLKNGAMPTTSQINVGRYLEFFRSYAEKQIPVLYVAFSSGLSGSYSSALQA
                     VELLKEEYEDPEIYVFDTKAASIGEGLLVLEAVRLKKEGKSIEETLEWLESNYLKVHS
                     WVTVDDLKYLERGGRISKASATLGSLLSVKPIITVDKEGRLTNVDKVRGRNKSIQTIV
                     NETVKTIVAPLDQIVYVAYAGDLESAEKAKRLLEEQIKMKDVKLYPLGPTIASHTGYG
                     CIAIFSMGISR"
     gene            complement(1468..3090)
                     /locus_tag="SCJ86_002541"
     CDS             complement(1468..3090)
                     /locus_tag="SCJ86_002541"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629689.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HEG1170051.1"
                     /translation="MITVNDVSLHFSDRKLFDDVNIKFTPGNCYGLIGANGAGKSTFL
                     KVLSGDLQPSTGSVTMGPDERMAVLKQNHYDYEDYTVLETVIMGHKRLYEVLKEKDAI
                     YMKEDFTDEDGIRAAELEGEFAELNGWEAEPEAAVLLQGLNIPEELHHQQMSELTGGQ
                     KVKVLLAQTLFGKPDVLLLDEPTNGLDIQSINWLEEFLINFENTVIVVSHDRHFLNKV
                     CTHMADLDFGKIKLYVGNYDFWLESSQLAAKLQANANAKKEEQIKELQEFIARFSANA
                     SKSKQATSRKKMLEKITLDDIQPSSRRYPFVGFKPEREIGNDLLQVENVSVTIDGKKI
                     LDDISFNLSKEDKVAFVAENDITTTTLFKVIMGEITPDTGTVRWGVTTSNSYLPKDTT
                     KEFDTDLTILDWLRQYASKEEDDNTFLRSFLGRMLFSGEEVLKPVNVLSGGEKVRCML
                     SKMMLSKANVLVLDDPTNHLDLESITALNDGLMAFTGSILFASHDHQFIQTLANRIIA
                     VSDKGVIDRADTTYDEFLENADIQKQLNELWA"
     gene            3339..3860
                     /locus_tag="SCJ86_002542"
     CDS             3339..3860
                     /locus_tag="SCJ86_002542"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342318.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF308 domain-containing protein"
                     /protein_id="HEG1170052.1"
                     /translation="MDEKRTIDWGSLILGILFVLVSLLSFQDPVGNLVAIVVVFAIFA
                     FIKGIFELFVRNRLKELTGYKGKMPLIIGIIDILVGVFFLFNIGAGVAALPFVFAVWF
                     IADSVLALFTADLARGVSEGYYWFTIIVNILGILLGIFLLFNPISSALTLSFLVGFYF
                     MLFGITHIVYAFR"
     gene            complement(4046..5776)
                     /gene="spxB"
                     /locus_tag="SCJ86_002543"
     CDS             complement(4046..5776)
                     /gene="spxB"
                     /locus_tag="SCJ86_002543"
                     /EC_number="1.2.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343304.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate oxidase"
                     /protein_id="HEG1170053.1"
                     /translation="MSKINAGIAMVKVLESWGIDHIYGIPGGSFNSIMDALYQEKTEI
                     NYIQVRHEEVGALAAAADAKLTGKVGAVFGSAGPGATHLINGLYDAQMDHVPLVAFLG
                     QVASTSMNYNSFQELNENPIFADVSVYNRTVMTPQSLPHVVDEAIKAAYEHKGVAIVT
                     IPVDLGFEEIDEQPFSTAHTHKTSVILPEEKDLLAALPYLEKAKKPVLYVGQGTRNGF
                     PQIKEFSEHFSVPVVASVLAKGIIPDDYENFLGFAGRVATKPGNEALAEADLILFVGS
                     DFPFGRAFFNPTAEFIQIDIDASKFGRRHETSLSILGDANTALQRLVELGDARPADSW
                     YLANQENKEKWVNWLKSFEDREEEPIRPEAVYKEINRIAEDDAIFVTDVGNTTIHSIR
                     LLNMNGKQKHTTSGWFATMGNGVPGGIAAQLSYPEKQVFTLSGDGGFAMVMQDIITQV
                     KYQLPIINVVFSNDSFGFIEAEQEDTEQKKFGVFLEDADFGKVGEALGANSFTVTEYS
                     QLRFAFDAAAKSDRPVVIDVKINNKRPLPVEDLHLDPRKYSEEEIQAFKEKYEVHDLP
                     VLNELYEKRL"
     gene            5941..6759
                     /locus_tag="SCJ86_002544"
     CDS             5941..6759
                     /locus_tag="SCJ86_002544"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002354454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ZIP family metal transporter"
                     /protein_id="HEG1170054.1"
                     /translation="MVEWLLRLEPWQQALAGTAFTYFMTALGAGLVFFFKEIKKEVLN
                     LMLGFASGVMIAASFWSLLDPAITKAEENGDIAWLVVSIGFGLGGVFLYMADKTLPHM
                     HFGPQHEAEGLPTHLKRTILLVFSITLHNIPEGLAVGVAFGAAATADNPTAAILAAVS
                     VALGIGIQNFPEGAAVSIPLRQEGLSRKKAFVYGQASGIVEPIAGVIGALLVTKVELL
                     LPYALAFAAGAMIYVVVEELIPEAQQTLSSKRHFAVFGVMSGFIIMMILDVALG"
     gene            complement(6862..7059)
                     /locus_tag="SCJ86_002545"
     CDS             complement(6862..7059)
                     /locus_tag="SCJ86_002545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328014.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEG1170055.1"
                     /translation="MRQKNMFLNIFMVLLVLIAASFVISLFVSVLSGLLWFAVKVLIP
                     IALIIWLVRWIKGNTHRKNYY"
     gene            7228..7806
                     /locus_tag="SCJ86_002546"
     CDS             7228..7806
                     /locus_tag="SCJ86_002546"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292646.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1211 domain-containing protein"
                     /protein_id="HEG1170056.1"
                     /translation="MPKNRLEAFTDAVIAIIMTILVLELHEPNGDTWEALAALGYRFF
                     IYIVSFIILAIYWNNHHHLFQVVHKINGRVLWANSFFVFALSLFPFATSWISEYINSL
                     APEMTYGFVTLTANIAYYILAKELVKANKGHEKALKAMLNNYKKSYLSIGLNVLGIVL
                     GYLVAPVAVLIANIVILAAWIIPSRTIESQYR"
     gene            7901..8038
                     /locus_tag="SCJ86_002547"
     CDS             7901..8038
                     /locus_tag="SCJ86_002547"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEG1170057.1"
                     /translation="MDQKRIHVGLITSFVTMLIYLDQGHLTWTVVGVVGFLTLFTAIK
                     K"
BASE COUNT         2460 a         1480 c         1619 g         2650 t
ORIGIN      
        1 ccactaaacg gtgttctcct gccaactcct cggtcacaag tcacaatatt caacaaacat
       61 gagaagctgt tttttgaaat tgttccttgt gactcggagc taaacggcag gctcacaacc
      121 tcttggtaaa cggtgttcaa tcagctgacc ctcggtatta gctcaaaaaa cggcaaaata
      181 tgaggaacta ttttttccgt ttttttctct aatactcagg tcaaaacgct gatttcacaa
      241 cctctgattc acggtgttcc agaagtagct tctacggaaa taagccgaaa tttcacaaaa
      301 atttgagaag caattttcgt aaattccttc ttatttcttg aagctgacca cttctgtcac
      361 aacctcttat tttgattaga acttgcgaaa ataaaccggt taaagtgtgt ctagcgcaag
      421 agatttgcta taatagacga taagggtaaa atgtaacgag aaggggttga aaaatgtgta
      481 tcaaattgta acagattctt gttgtgatct gccataccat gtattagcaa aagaaaaagt
      541 tgatttttta tcaatgaatc tccaattgaa tgatcaagag ttagtagatg acctcggaaa
      601 gacattcaat tatgaccgct acatgaatca gctgaaaaat ggtgcgatgc ctacgacatc
      661 acaaatcaat gttggccgat atttagaatt tttccgttct tatgcggaaa aacagattcc
      721 tgttttatat gtggcttttt ccagtggatt aagcggctct tacagtagtg cgcttcaagc
      781 ggttgaattg ctgaaggaag aatatgaaga ccctgagatc tatgtttttg atacaaaagc
      841 tgcaagtatt ggggaagggc ttttagtatt ggaagctgta agattgaaaa aagaaggtaa
      901 aagcatcgaa gaaaccctag agtggctgga aagtaattat ttgaaagtcc attcatgggt
      961 aacagtagac gatctgaaat atctagaacg aggcggtagg atttccaaag caagtgctac
     1021 tcttggcagt ctattgagtg taaagcctat tattacggtg gataaagaag gaaggctgac
     1081 gaatgttgac aaagttcgag gaagaaacaa gtcgatccag acaatcgtca atgagacggt
     1141 aaaaacaatc gttgcaccac ttgatcaaat cgtttatgtg gcttacgctg gggatctaga
     1201 gagcgcggag aaggcaaaac ggcttttgga agagcagata aagatgaaag atgtgaagct
     1261 ttatccctta ggaccaacga tcgccagtca cacaggctat ggctgtatcg cgattttttc
     1321 gatgggaatc agtcgataaa agcagtaact cattctgtaa aaaagcccag aaattgattg
     1381 taacaaaaac agcgactctt aaaagataga tgagcatcct atctcttaga ggtcgctgtt
     1441 tttttatttt ttaagctaaa taggccatta tgcccataac tcgttcaatt gtttttgaat
     1501 atctgcattt tccaaaaatt catcatatgt cgtatcagct cgatcgatca cacctttatc
     1561 cgaaacggca atgatgcggt ttgccaatgt ttggataaat tgatggtcat gtgacgcaaa
     1621 taagattgag ccagtaaatg ccatcaagcc gtcattcaat gctgtgattg attctaagtc
     1681 taaatggttc gttggatcat ctaatacaag tacgttagct ttcgaaagca tcatttttga
     1741 aagcatgcaa cggactttct ctccaccaga taaaacattc actggtttca atacttcttc
     1801 accagaaaac aacattcttc ctaagaagct tcttaagaag gtgttatcat cttcttcttt
     1861 acttgcatat tgacgcaacc agtctaaaat cgttagatca gtatcgaatt ctttcgttgt
     1921 atcctttggt aaataagaat tacttgttgt cacaccccaa cgtactgttc cagtatctgg
     1981 cgtaatttca cccatgataa ctttgaatag cgttgttgtt gttatatcat tttctgcgac
     2041 gaaagcgacc ttgtcttctt tgctcaaatt aaaagatata tcatctaaaa ttttctttcc
     2101 atcaatcgtt acacttacat tttctacctg caataagtcg tttccaatct cacgttcagg
     2161 tttgaatcca acgaatggat aacgtctaga agaaggttgg atatcgtcta aagtaatttt
     2221 ttcaagcatt tttttacgtg aagttgcttg ttttgatttt gatgcattcg cactaaagcg
     2281 ggcaataaat tcttggagtt ctttgatttg ttcttctttt ttggcatttg cgtttgcttg
     2341 aagtttagcc gctagttgac tagattccaa ccaaaaatca taatttccta cataaagttt
     2401 gattttacca aaatctagat ccgccatatg tgtacatact ttgttcaaaa agtgacggtc
     2461 atgggataca acgatcacag tattttcaaa attgatcaaa aattcttcca gccaattgat
     2521 cgattggata tccagaccgt ttgttggttc gtctagcaaa agaacatcag gtttaccaaa
     2581 tagtgtttgt gctaataaca cttttacctt ttgtccacca gttaattcac tcatttgctg
     2641 atggtgcaat tcttcaggga tgttcagacc ttgcagtaaa acagctgctt ctggttcagc
     2701 ttcccatcca ttaagctcag caaattctcc ttctaattca gctgctcgaa taccatcttc
     2761 gtcagtgaaa tcctctttca tgtaaatcgc atctttttct ttcagcactt catacagacg
     2821 cttgtgtccc atgatgactg tttcaagtac agtatagtct tcatagtcgt aatggttctg
     2881 tttcaatacg gccatccgtt catctggtcc catagtaacg gaacctgtgg aaggctgcag
     2941 atcccctgat aacactttaa ggaacgttga tttacctgca ccatttgcac caatcaagcc
     3001 atagcagttt ccgggggtaa atttaatatt cacatcatca aatagtttgc ggtctgaaaa
     3061 gtgcaaactc acatcattta cagtaatcaa tttttttcct cacttttatc attattagtt
     3121 ttgacaagtc ttatagaatt atatagggga aaggtaaaag attcaaggca atcgaacatt
     3181 ctttgtgttt cacgtgaaac agaatgaaaa aaataaaaat aaaagcggag cgaaaatagc
     3241 tactatagga tttattagtt tgcaacagca taatttttga cgttatttat aagagggagt
     3301 actataaaac tgtaaaagaa atgaaagaag gattgaaaat ggatgaaaaa agaaccatcg
     3361 actggggctc attgattttg ggaattttat ttgtacttgt ctcattgtta tcatttcaag
     3421 accctgtcgg aaatttagtt gcaattgtag tagtctttgc gatttttgcc tttattaaag
     3481 ggatatttga actgtttgta cggaacagat taaaagagct aacaggatat aaaggaaaga
     3541 tgcccttaat aatcgggatc atcgatattc tagttggcgt ctttttctta ttcaatattg
     3601 gtgcaggagt cgcagcattg ccttttgttt ttgcagtatg gtttattgct gattcagttt
     3661 tagcgttatt tactgctgac ttggcaagag gagtaagtga aggatattat tggtttacga
     3721 tcatcgtcaa tattttagga attttattag gaattttctt attgtttaat ccaatttctt
     3781 ctgcgttgac attaagcttt ttagttggat tctattttat gttatttggt atcacacata
     3841 tcgtctatgc atttagataa gtaaaatcaa acaaataaaa aagccggctt ccgattacag
     3901 ttttactgtt gtaattggaa gccggctttt ttatcatgga caaatatgca cgaggtgatt
     3961 tttaggtgtc gagacaaatt tatgcaaaaa tgtgagaagc tatatacaca aattttatct
     4021 taatactcga agcatagcgc agttgtcaca acctcttttc atataattca ttcaatactg
     4081 gcaaatcatg gacttcatat ttttctttga atgcttggat ttcttcttca gaatattttc
     4141 ttggatctaa atgcagatct tctactggca atgggcgctt gttattgatc ttcacatcga
     4201 ttactacagg acgatctgat tttgcagctg catcaaaggc aaatcgaagt tgcgagtatt
     4261 ctgtgactgt aaagctattt gccccgagcg cttctccaac tttaccgaaa tctgcatctt
     4321 ctaaaaatac accaaatttc ttttgttccg tatcttcttg ttctgcttcg atgaaaccaa
     4381 aagaatcatt cgaaaagaca acattaataa ttggaagctg atatttgact tgtgtaatga
     4441 tatcttgcat gaccatagca aacccgccat caccacttaa ggtaaatact tgtttttctg
     4501 ggtagcttag ttgtgcggca attcctcctg gcacaccatt acccatagtt gcgaaccatc
     4561 cagatgtcgt atgtttttgt ttaccgttca tattcaataa acgaatagaa tggatagtcg
     4621 tgttaccgac atctgtgacg aaaatcgcat cgtcttcagc aatacgattg atttctttat
     4681 agacagcctc tggacgaatc ggttcttcct ctcgatcttc aaaagatttc aaccaattta
     4741 cccatttttc tttattttct tgatttgcta ggtaccagct atcagcaggg cgagcatccc
     4801 ctaactcgac aagtctttgc aaagctgtgt ttgcatcacc gaggatcgac aaagatgttt
     4861 catggcgtcg tccaaatttt gacgcatcga tatcgatttg gataaattca gcagttggat
     4921 taaaaaatgc acgtccaaat gggaagtcgc taccgacaaa taagatcaga tcggcttctg
     4981 ccaatgcttc atttccgggt ttagtagcga cacgtccagc aaaaccaaga aagttttcat
     5041 aatcatcagg aataattcct tttgctagaa cggaagcgac aacaggaact gaaaaatgtt
     5101 ctgagaattc tttgatttgc ggaaatccgt ttcgcgttcc ttgaccaaca taaagaactg
     5161 gtttttttgc tttttcaaga taaggcaaag cagctagcaa atctttttct tctggtaata
     5221 tcacacttgt tttgtgagtg tgagcggtgg agaaaggttg ttcgtcaatt tcttcaaaac
     5281 caagatcaac agggatagtc acgatagcca cgcctttatg ctcataggcg gctttaatgg
     5341 cttcatctac tacatgcgga agactttgtg gtgtcatgac tgtacgattg tatacgctaa
     5401 catctgcgaa aataggattt tcatttagtt cctggaaaga gttatagttc atggatgtag
     5461 aagcaacttg tcctaagaac gctactagag gcacatggtc catctgtgca tcatacaaac
     5521 cattgatcaa atgtgtcgca ccaggtcctg cagagccgaa gacagcgccg actttccccg
     5581 ttaattttgc atctgcagcg gctgcgagtg cgccgacttc ttcatgacga acttgaatgt
     5641 agttgatttc ggttttttct tgatatagcg catccataat tgaattgaaa gatcctccgg
     5701 gaatgccgta aatatggtcg ataccccagc tttcaagaac ttttaccatt gcgattcctg
     5761 cattgatttt tgacatggtg tttcactcct ttagcaatga tgatacataa attttttgta
     5821 tctagagtat agaacttttt atcgaaaatc ccaactaatt cgtcttttca caaaaatgta
     5881 gtcatgtttt tgggaatatg ctagagtagt acatagatag attcaagaga ggggaatggc
     5941 atggttgaat ggctattgag acttgaacct tggcagcaag cacttgctgg gacagctttt
     6001 acttatttta tgacagctct tggtgccggg ttggtttttt tctttaaaga aatcaaaaaa
     6061 gaagtgctaa acttgatgtt aggatttgct tctggtgtca tgattgctgc aagtttttgg
     6121 tctttgttag atccggctat tacaaaagca gaagaaaatg gagatattgc ttggctagtt
     6181 gtaagtattg ggtttggttt aggcggtgtg tttttatata tggcagataa aacactgccc
     6241 catatgcatt ttggaccaca acatgaagca gaaggattgc ctacccattt aaaacggacg
     6301 atcttgcttg ttttttctat tacattgcat aacattcctg aaggtttagc cgtgggggta
     6361 gcattcggtg cagcagcaac tgcggataat ccgacagccg ctatattagc cgctgtttcg
     6421 gtggcgctag gtattggtat ccaaaacttc ccagaaggag cagctgtatc aatcccgttg
     6481 cgacaagaag gtctgagtcg taaaaaggcc tttgtttacg gacaagcttc tggaatcgtt
     6541 gaaccaattg ctggagtgat tggagcgcta ctagtaacga aagtggagct gctgcttcct
     6601 tatgcacttg cttttgccgc aggagcaatg atttatgtcg tggtggaaga attgatccct
     6661 gaagcacagc agacattgtc cagcaaacgt cattttgcag tttttggcgt catgagtgga
     6721 tttatcatta tgatgatttt ggatgtggca ttaggataaa taaaaaaaat atatatgata
     6781 tatcaaatag ggccgcgaac ttatatcgcg accctatttt tttagtaagt tatatcataa
     6841 tgaagtgaat ggtatcatac attaataata gttttttcta tgtgtatttc ctttgatcca
     6901 gcgaacaagc cagatgataa gtgcgattgg gattagaact ttgaccgcaa accataaaag
     6961 cccgcttagg acactgacaa aaagactgat gacgaagcta gctgcaatta gcactaaaag
     7021 aaccatgaag atatttaaga acatattttt ttgacgcata caaaaccctc ctcttgttca
     7081 ttctttacta tagaaaaagt ataacaggga ttgtttaaaa acacctccaa ctgaagactg
     7141 atttttactt catctactta aatgatcaaa caattgcttt ttctctggtt tatcatctaa
     7201 aatagagcgg aaaaggagga gaatatcatg cctaaaaatc gtcttgaagc atttacagat
     7261 gctgtaatcg cgattatcat gactatttta gttttagaac ttcatgaacc taacggagac
     7321 acgtgggagg cgctcgctgc attaggttac cgatttttta tctatattgt cagttttatc
     7381 attttagcga tttattggaa caatcatcat catttatttc aagttgtaca taagataaat
     7441 ggacgggtct tatgggcgaa cagtttcttt gtttttgcgc tttcgctttt tccgtttgca
     7501 acttcatgga tcagtgagta catcaatagc ctggctccag aaatgactta tggttttgtt
     7561 accttgaccg ctaatattgc gtattatatc ttggcaaaag aacttgtcaa agcaaataaa
     7621 ggccacgaga aagcattgaa ggcgatgttg aataattaca agaaatcgta tctatcgatt
     7681 ggtttgaatg tgttaggaat tgtcttaggg tatttagtag cgccagtagc cgtgctgatc
     7741 gcaaacatcg tgattctagc tgcttggatc atcccaagtc gtaccatcga atcacaatat
     7801 cggtaaaatg atccgtgttt aaaatttaag agaaaaatat agaaaaaata aataaacgat
     7861 caaatatccg agtgatacta gtattcaaag agggatgcag atggatcaaa aaagaattca
     7921 tgtaggactg attactagtt ttgtcacgat gcttatttat ttggatcaag gtcatttgac
     7981 ttggacagta gttggtgtcg taggattttt gacattattc acagcaataa aaaaataaac
     8041 atctttatgt aatctgatgt tctatctatt taagaaaagc atgtttacag cagtatgatt
     8101 ctgttgtaaa cgtgttttta tttgtggaaa atttaatttt tagaaaagtg tatcgaaaaa
     8161 tagatagtta gtttttccac agtggataac ttcgtaagag ataaaaata
//
LOCUS       DARBET010000099         7926 bp    DNA     linear   BCT 07-NOV-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4027
            SAMEA114569841-rid20112183.denovo.102, whole genome shotgun
            sequence.
ACCESSION   DARBET010000099 DARBET010000000
VERSION     DARBET010000099.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA114569841
            Sequence Read Archive: ERR12203666
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 7926)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7926)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001972618.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001972618.1
            Genome Coverage       :: 132x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/07/2023 13:18:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,041
            CDSs (total)                      :: 2,975
            Genes (coding)                    :: 2,908
            CDSs (with protein)               :: 2,908
            Genes (RNA)                       :: 66
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 67
            CDSs (without protein)            :: 67
            Pseudo Genes (ambiguous residues) :: 0 of 67
            Pseudo Genes (frameshifted)       :: 26 of 67
            Pseudo Genes (incomplete)         :: 28 of 67
            Pseudo Genes (internal stop)      :: 20 of 67
            Pseudo Genes (multiple problems)  :: 6 of 67
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7926
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA114569841-rid20112183.denovo.102"
                     /isolate="VRE4027"
                     /db_xref="taxon:1352"
                     /country="Denmark"
     gene            complement(<1..199)
                     /locus_tag="SCJ86_002548"
     CDS             complement(<1..199)
                     /locus_tag="SCJ86_002548"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002395661.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEG1170058.1"
                     /translation="MKKMGKCILCEKYTELTKEHVPPKKSGNTGGKKTRTGNLDDFLK
                     SDFTKGDFPKGIKRKPQGNVYY"
     gene            complement(362..1996)
                     /locus_tag="SCJ86_002549"
     CDS             complement(362..1996)
                     /locus_tag="SCJ86_002549"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328113.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HEG1170059.1"
                     /translation="MEMIQTKRVALYIRVSTQEQALEGHSIQAQEQNGRQFAERMGYE
                     VVEVYADEGISGKSTKNRFAYQRMMNDARNHRFDLLVIWKLTRLGRNMLDVLQTVEEL
                     LRYDVGLHSISEQFDVTTSTGKLMLQLLGSFGEFERNQIAENVQMSMQSLVKDKKRYA
                     GGRRLGYVSGIDTNGQKQLVIEPEEAKIVRLIYAKYLGGAGYRAIANFLNRQGYQTVK
                     KNAFSTTAVKDILRNKIYAGYLEYARYINWETKRRKGKNPRPILVKGTHEPIISEEDY
                     QKVQERLALEGKQPKWNHTGENVLTGLLRCPECGAPMAASNVTNTLKDGTKKRIRYYS
                     CSVFRNKGASVCHANSIRAEQAETFVADRLKEIVQLPEVLPRLVAALNEEIVRQSQPL
                     EQELVVLLERKEELKTKIEKWEAALEDSPELFPMLKDRLDELTEKRRQLHIRENEILG
                     IFQQGEPIQVKDVQRILTNLDRFLAQSEKKQIKALYRTFIEKITFDPNHKEMLEITMK
                     FTHAVVQQLNEQYQIAVSKTKDTAIFVLKTPFTLTI"
     gene            complement(2000..3373)
                     /locus_tag="SCJ86_002550"
     CDS             complement(2000..3373)
                     /locus_tag="SCJ86_002550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HEG1170060.1"
                     /translation="MRVACYDRTSVYEEAKISNDAQYDAVKEYVLEKYEQIPQRFSDK
                     GESGEKAERPAFRQLRAWVCASEEEGVIYVWRYDRLARQAKIALEFIELCQQHQVQVY
                     SISEPLPASFSHASTNKLFVQLLFTFAEQQRNVIIENVRAGLSYKKQQRQYLSSAVPY
                     GYRLVNGKIQQEVHEARIVRYIFQLYLTKKYGYKKLCQRLTQQKLFFRERPFQPYHIY
                     SILKNPLYYGEIKGGSLGKYLGTFEPILSKATFLQAQEIRQSRCTAKKDTYLYLLRQK
                     IRCPFCGRHLSSKYQWNTKKTKTLHYYHCTDRSCQGIFLRAERVESEVLEKIQAFFQY
                     EGVYQALIAQLTQQLAEKRKDEKRKVIQQKKQEAQWIKAFEEGNCSLEELKQQLQALQ
                     KPMSTSSFLSLQQCEAKVKEWLQLKDLSSRQLLCDYVCRIEVTKDKTVCGIYFRQLTQ
                     NILERNE"
     gene            complement(3389..3628)
                     /locus_tag="SCJ86_002551"
     CDS             complement(3389..3628)
                     /locus_tag="SCJ86_002551"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312623.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEG1170061.1"
                     /translation="MTKNKMTLKAEVLLYIQEHFSNQAFFTKPIYLDFEIRGVSAGSI
                     GGTLQALKNEGYLENHFVQRSFNRRVVKKWYLVHS"
     gene            complement(3810..6485)
                     /locus_tag="SCJ86_002552"
     CDS             complement(3810..6485)
                     /locus_tag="SCJ86_002552"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324323.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF927 domain-containing protein"
                     /protein_id="HEG1170062.1"
                     /translation="MKMNHTSIQKHLQLLNTLGISYVNLRTFSTLNKKTVSHIFSTAD
                     SQAIINYLEKQKLSEGTVNITYNPIKQEVITQKKHSVRDNDIEKIRFVFIDIDPKRKE
                     GSATDSEKKKAENVMEQVERYLKEKRIESFVKVDSGNGYHIFLPINEQPNNHETILTI
                     QNFLQLLHRRFGVEDEVDIDTSVYNPSRLCKLVGTPAVKGKSTKERPHRLSSFLSIPD
                     NIDRSNCFKILEQIVEENSMMSTSRVKSHPSFREVLPFIQADYKQWLAHYPELTYTIK
                     EGDHKGVTLLIFDECPLRKHTNNSNGSCLSVTADNKIRFHCLHASDKNKDIQDFVKKY
                     PLPKPVNPSVESLESNQEYYFDQFKLNQEGVFLVKEGQEEKVACPMFIKQIRKNIEQK
                     VITLVLAYYVDHCWDEIEIEGGCLQTNNFKQLAKHGLIIKARREAEVVDFLQIQRENA
                     PIFFEHESLGWKKENEAMIFQLQDSFGREIPSVLTKNSCYQLTTNGTEAEFDALVHKY
                     VLGTNLELAWSIGIASIVLGYLNVTKANTFLSLLINLQGKSTTGKTTTLHFIASLFGN
                     PTTVLRNMNATNKAIIKLASNNQGGVPLILDELGAATQNNFSSLIYQLASGEERLRLD
                     SNCQLMPQETFSVLTLTSSEERLENYFNEKLTGLKVRYIEFSDIKFTKDANHAEKVKS
                     LSSNTFGWGIKLFLLKLFEQGITKIEATFVEAQKKLWAVLPEHSLKQRIGNNLALIYS
                     GALLIQDILGYPLRLEHIQTVLINSYEQLIVDTSLGSEQTVFDRVKTLLVMNSHKFLT
                     NSDNKHTPSTIWGKVSIQKNGDIKVNIFPIPFEDLLRREFQVKDLTYLFRQLLDSGNM
                     CAEKGRRTKRIHMNRQVLPTYEILLPSSLKSYFRL"
     gene            complement(6668..7591)
                     /locus_tag="SCJ86_002553"
     CDS             complement(6668..7591)
                     /locus_tag="SCJ86_002553"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333866.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEG1170063.1"
                     /translation="MATNYAKKINFDVDKLYKSLIVVPKNKSEDGNYELEVLQFKIEQ
                     KSSERFMSNQLVKIKKWLPMIDQLVSNNKEAKNLCIFVLLLHGRVINLVNRKSTTKFA
                     NSFWFLKNINKTVEKNGVYKGDFHFQNAETIEREHHYFDLLVNTVVELAKWAKSNETY
                     SQVLKRIMSQETSFIYELSKSNLKYTYNEDELEILDNPENSQWIKELKREDFIETLRI
                     LKSLFDLKLAKATIMGIERDSYYWEEIGLYAPQIEGEDKEKRIDNIINYINSLSEPLK
                     CRGLDFIGTLGWTETYYEKIYKEIIEQYFPK"
BASE COUNT         2328 a         1560 c         1198 g         2840 t
ORIGIN      
        1 tataataaac atttccttga ggtttccttt taatcccctt cggaaaatca ccttttgtaa
       61 aatcgctctt aaggaaatca tctaaattac cagttctggt ctttttacct ccagtattcc
      121 ctgatttttt aggtggtaca tgttctttag taagttcagt gtatttttca cataaaatac
      181 acttacccat ctttttcata tctatcccca ctctttttaa ttcttatata caattatatt
      241 tcaaatattc attaataaat atccctattt atgataagga cctccctaac tacttctcta
      301 acgatttcaa atcgctagag aagcgttagc gcccctctaa cgttgtttga tataacctag
      361 actagatcgt taacgtaaag ggtgttttta acacaaaaat agccgtatcc tttgttttgg
      421 atacggctat ttgatattgc tcattcaatt gttgtaccac ggcatgcgtg aatttcatcg
      481 taatttccaa catctcctta tggttaggat caaaggttat tttctctata aaggtacgat
      541 acaaggcttt gatttgcttc ttctcactct gggcaagaaa acgatccaaa ttagtcaata
      601 tccgttggac atctttgact tgaatcggct ctccttgttg aaagatccct aagatttcat
      661 tttctcgaat gtgcaactgt cgtcgttttt cagtcaattc gtctaagcgg tcttttaaca
      721 tcggaaacaa ttcaggacta tcttccaaag cagcttccca tttttcaatt ttagtcttca
      781 attcttcctt tcgttccagt aacaccacga gttcttgttc caatggttgg ctttgtcgca
      841 ctatttcttc atttaacgct gccaccaatc ggggaagaac ttctggtagc tgaacgatct
      901 cctttaatcg gtcggcgaca aatgtttccg cttgttcggc acgaatcgaa ttggcatgac
      961 acacacttgc tcctttattc cgaaagactg aacaagaata atatcgaatc cgtttctttg
     1021 tgccatcctt tagggtattt gtgacattac tcgctgccat cggtgcgcca cattctggac
     1081 aacgtaacaa gcctgtcaat acattttcac ctgtatggtt ccatttgggt tgttttcctt
     1141 ctaaagcaag gcgttcttgt actttttgat aatcttcttc cgaaataatc ggttcatggg
     1201 ttcctttcac taaaatcggt ctcggattct tccccttccg tcgtttggtt tcccaattga
     1261 tgtagcgcgc atattctaaa taccctgcat aaattttatt gcgtaagata tctttgactg
     1321 ccgtggtaga aaaagcattc tttttgactg tttgatatcc ttgacgattc aaaaaattgg
     1381 caatcgctcg ataccctgct ccgcctaaat attttgcata gatcagacga acaattttcg
     1441 cctcttctgg ttcaatgacc aattgttttt gaccattcgt gtcaatacca ctgacatagc
     1501 ctaaacgtcg gcctcctgca taacgctttt tatctttcac taagctttgc attgacattt
     1561 gaacattctc cgcaatttgg ttgcgttcaa attcaccgaa actcccaagg agttgtaaca
     1621 tcaatttacc tgtcgaagtg gtcacgtcaa attgttccga gatcgaatgt aatcctacgt
     1681 catatcgcaa taattcctcg actgtttgta agacatctaa catattgcgt ccgagacggg
     1741 tcaatttcca aatcactagt agatcaaaac gatggttccg agcatcgttc atcattcgtt
     1801 gataggcgaa ccgatttttt gtggatttcc cactgattcc ttcatcggca taaacttcta
     1861 ccacttcata gcccatccgt tcggcaaact gtcgtccatt ttgttcttgt gcttgaatac
     1921 tatgtccttc taaagcttgt tcttgtgtac tcacgcgaat atacaatgcg actcgtttgg
     1981 tttgtatcat ttccatagat cattcgttcc tttctaaaat attttgtgtc agttgtcgga
     2041 agtaaatccc acaaactgtt ttatcctttg ttacctcaat ccgacaaaca tagtcacata
     2101 ataattgtcg actagagagg tcttttagtt gaagccattc tttcactttt gcttcacact
     2161 gttggagtga aagaaacgaa gaagtagaca tcggcttttg aagagcttgc agttgttgct
     2221 tgagttcttc cagtgaacaa ttgccctctt caaatgcctt aatccattgt gcttcttgtt
     2281 tcttttgttg gattactttg cgtttttcat cttttcgctt ttcggctaat tgttgcgtta
     2341 attgggcaat caaggcttga tagacgcctt cgtattgaaa aaaagcctgt atcttttcca
     2401 atacttctga ctcaaccctt tctgcccgaa gaaaaattcc ttggcagctt cggtcggtac
     2461 aatgataata atgaagcgtc ttcgtcttct ttgtattcca ttgatacttc gaactcagat
     2521 gacgaccaca gaagggacac cgaatttttt gacgtaataa gtacagatac gtatcttttt
     2581 tagctgtaca ccttgattgg cgaatctctt gggcttgtaa aaaggttgct ttcgagagaa
     2641 tcggttcaaa ggttcctaga tacttcccca aagatcctcc tttgatttcg ccatagtaca
     2701 atggattttt taggatacta taaatatgat agggctgaaa aggacgttcg cggaagaaaa
     2761 gcttttgttg cgtcaatcgt tgacaaagct ttttgtagcc atattttttg gtcaaataaa
     2821 gttgaaaaat atagcgaaca atccttgctt cgtgtacttc ttgttggatt ttcccattca
     2881 ctaaacgata cccatatggt accgcagaag acaaatattg ccgttgttgc tttttatagg
     2941 aaagtccggc acgaacattc tcaataatga cgtttcgttg ttgttctgca aaagtgaaca
     3001 ataattgcac aaatagttta ttcgttgacg cgtgtgagaa agaagcagga agaggttcag
     3061 aaatcgaata cacttgaacc tgatgttgtt ggcacaactc aataaattct aaggctattt
     3121 tggcttgacg tgccaatcga tcataacgcc aaacatagat caccccctct tcttctgacg
     3181 cacaaaccca agctctaagc tgtcgaaaag ctggacgctc cgctttttcc cctgattctc
     3241 ctttgtctga aaatcgttgg ggaatttgtt catatttctc taaaacatac tctttcactg
     3301 catcatattg agcgtcatta ctaattttcg cttcttcata aacagacgtt cgatcataac
     3361 aagccacacg catgcttgat tcccctctct atgaatgaac taaataccat tttttcacga
     3421 ctcttctatt gaacgaacgt tgcacaaagt gattttctaa atatccttcg ttcttcaacg
     3481 cttgaagagt acccccgata gatccagcag atacccctct tatttcgaaa tccaagtaaa
     3541 ttggttttgt aaaaaaggct tgatttgaaa aatgttcttg aatatacaga agaacctctg
     3601 cttttagtgt cattttattt ttagtcatta gaattttcct ttctttcatg tttgattgaa
     3661 tgaagtaaat gaagcataga tcaagcttca tttacttccg tttgggtttg ctcgctatca
     3721 tacagataag taatcagttg ttttgctaaa tcaataagaa atatttccat aacatggtgc
     3781 ctcactttca tactttggtt tattctaaat tacaatcgaa aatacgattt gagtgaagaa
     3841 ggtaatagaa tctcgtatgt tggaagaact tgacgattca tgtgaatacg tttcgttctt
     3901 ctcccttttt ctgcacacat attacccgaa tcaagcaatt gcctaaaaag ataggttaga
     3961 tcctttactt gaaattctcg ccttaacaag tcctcaaacg ggataggaaa aatatttacc
     4021 ttaatatccc catttttttg aattgatact tttccccaaa tcgtactggg cgtatgtttg
     4081 ttgtcactgt ttgttaaaaa tttatggcta ttcatcacca acaatgtttt cactcggtca
     4141 aataccgttt gctcacttcc taaactagta tctacaatta actgttcgta cgaattaata
     4201 agcaccgtct gtatatgctc taatcgtaaa ggatagccca gtatatcttg aattaggagg
     4261 gctccactat aaatcaaagc tagattatta ccaatgcgtt gcttcaaaga atgttcaggt
     4321 aaaacagccc atagtttttt ttgagcttcg acaaaagttg cttcaatttt tgtaatccct
     4381 tgttcaaaga gtttcaaaag aaataacttt ataccccaac caaaagtatt agaagaaaga
     4441 cttttaactt tctctgcgtg attcgcatcc tttgtaaact tgatatcaga aaattcgata
     4501 tatcgaactt tcaaaccagt taatttctca ttgaaataat tttccaaacg ctcttcagaa
     4561 gaggtaaggg tcaaaacaga aaaagtctct tgtggcataa gctgacaatt agaatctaat
     4621 cttagccttt cttctcctga agctaattga tagatcaacg agctaaagtt attttgagtt
     4681 gctgctccta actcatctag tattagtgga acacctccct gattgtttga tgctagcttt
     4741 ataatggctt tattggtagc attcatattt ctaagaaccg tagttggatt tccaaataaa
     4801 gaggcaataa agtgtaacgt agtggtttta cccgttgtgc tcttgccctg taggttaatt
     4861 agtaaactca aaaaagtatt agccttcgta acgtttaaat agcctaacac aatagaagca
     4921 ataccaatcg accaagcaag ttctaagttc gttcctaaaa catacttatg aaccaatgcg
     4981 tcaaattccg cttctgtacc attagttgtt aattggtagc atgagttttt agttaataca
     5041 gaaggaattt cacgtccaaa agaatcttgt aactgaaaaa tcattgcttc attttctttt
     5101 ttccagccaa gagattcatg ttcaaaaaag attggagcat tctcccgttg aatctgtaaa
     5161 aagtcaacga cttcagcttc tcttctagct ttaatgatta agccatgttt tgctaattgt
     5221 ttaaaattat tagtttgtaa acatccacct tctatttcta tttcatccca acaatgatcg
     5281 acataatagg caagaacaag tgtaatgact ttttgttcga tatttttgcg tatttgctta
     5341 ataaacatcg gacaagcaac tttttcttct tgaccttctt tcaccaaaaa tactccttct
     5401 tgattcagtt taaactggtc aaaataatat tcttgattgg attctaatga ttctacagaa
     5461 ggatttacag gttttggcaa cggatatttt tttacaaagt cttgaatatc tttattttta
     5521 tccgaagcat gcagacaatg aaatcgaatc ttattatcag ccgtaacaga caaacaagaa
     5581 ccattagaat tattcgtgtg ttttcttaat ggacattcgt caaatataag taaggtaact
     5641 cctttatgat cgccctcttt tatggtataa gttaattcag gatagtgagc taaccattgt
     5701 ttatagtctg cttgaataaa aggtaacact tctctaaaag atggatggct ttttactcta
     5761 gaggttgaca tcatggaatt ttcttcaact atctgttcaa gaattttaaa acaattactt
     5821 ctatctatgt tatcgggaat agaaaggaaa ctagataaac gatgcggacg ctcttttgtg
     5881 gactttcctt tgaccgctgg tgtaccaact aatttgcata atcttgaagg attataaaca
     5941 gaagtatcaa tatcaacttc atcttctact ccaaatcttc ggtgaagtaa ttgaagaaaa
     6001 ttctgaatag taagaatcgt ctcgtgatta tttggttgtt catttatggg aagaaaaata
     6061 tggtagccat tcccactatc aacttttaca aatgattcta ttctcttttc tttcaaataa
     6121 cgttctactt gttccataac attttctgcc tttttctttt cgctatctgt tgcacttccc
     6181 tctttccttt tagggtcaat atcaataaat acaaaccgaa ttttttcaat atcattgtct
     6241 ctcacagaat gctttttttg tgtaatcacc tcttgtttaa taggattata agttatgttt
     6301 actgtacctt cagacaattt ctgtttttct aaatagttaa taattgcttg agaatctgca
     6361 gtagaaaaaa tatgagaaac ggttttcttg tttaatgtag aaaaagttct caaattaaca
     6421 taagaaatcc ccagtgtatt taacagttgt aaatgcttct gaattgaagt gtgattcatt
     6481 ttcattaatt accactcact ttctaaaaaa tttttatttg tatgactaca ttacctccaa
     6541 cctctcataa tttcatgatc ttcagttcta caaaaatata tcccactcaa aatcttgaca
     6601 aaagaaaaaa gttgtgattt tggaaatcac aacttttttc ttttacgata aatcaatcct
     6661 tattaaatta tttaggaaaa tattgttcaa ttatttcttt atatattttc tcatagtatg
     6721 tttctgtcca tcctaatgtt cctataaaat caagtcctct acattttaaa ggttcactta
     6781 aactattaat atagttaata atgttgtcta tccttttctc tttatcttca ccttcaatct
     6841 ggggagcata tagtccaatt tcctcccaat aatatgaatc tctttcaatt cccatgatgg
     6901 tagctttggc taatttcaaa tcaaataacg attttagaat tcttagtgtc tcaataaagt
     6961 cttctctctt caactcttta atccattgtg aattttctgg attatctaaa atttcaagtt
     7021 catcttcatt ataagtatat ttcaaatttg acttgcttaa ctcatagata aatgaagttt
     7081 cttgactcat aattctttta agtacttgac tataagtttc gtttgacttt gcccatttcg
     7141 ctaattctac cactgtattc actaataaat cgaaataatg atgttctcgt tcaatagtct
     7201 cagcattttg aaaatgaaaa tcacctttat aaactccatt cttttccact gttttattaa
     7261 tattttttaa aaaccagaat gagtttgcaa atttagtcgt actttttcga ttaactaagt
     7321 taataactct tccatgtaat aataagacaa aaatacataa atttttagct tccttattat
     7381 tagatactaa ttgatcaatc atagggagcc attttttgat tttcactaat tgattgctca
     7441 taaatctttc actggacttt tgttcaatct taaattgcaa aacttctaac tcataattcc
     7501 catcttctga tttatttttg ggaacaacta tcaaactttt ataaagttta tccacatcaa
     7561 aatttatctt ttttgcataa tttgtagcca ttaatacatc tccttattca taataaattt
     7621 catatgacgc tcctctcaaa atttaccggt agactagaaa aaagaataaa agatacgtca
     7681 attcacactt tattcttaaa aaaaaactgg tctctctacc ggtaatatcg attgagtccg
     7741 cctttatcga ctttatcctt tttttatatt aaattttttc accggtagac agggagagaa
     7801 agaataaact aaaaagccct taattcttaa tgttttcacc tccttaccgt tcctaccgct
     7861 aaaatttaat ataactaaat tctataacat tagaagaaat tttaaatacc tttgataaaa
     7921 aatatt
//
LOCUS       DARBET010000100         7601 bp    DNA     linear   BCT 07-NOV-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4027
            SAMEA114569841-rid20112183.denovo.103, whole genome shotgun
            sequence.
ACCESSION   DARBET010000100 DARBET010000000
VERSION     DARBET010000100.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA114569841
            Sequence Read Archive: ERR12203666
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 7601)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7601)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001972618.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001972618.1
            Genome Coverage       :: 132x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/07/2023 13:18:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,041
            CDSs (total)                      :: 2,975
            Genes (coding)                    :: 2,908
            CDSs (with protein)               :: 2,908
            Genes (RNA)                       :: 66
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 67
            CDSs (without protein)            :: 67
            Pseudo Genes (ambiguous residues) :: 0 of 67
            Pseudo Genes (frameshifted)       :: 26 of 67
            Pseudo Genes (incomplete)         :: 28 of 67
            Pseudo Genes (internal stop)      :: 20 of 67
            Pseudo Genes (multiple problems)  :: 6 of 67
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7601
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA114569841-rid20112183.denovo.103"
                     /isolate="VRE4027"
                     /db_xref="taxon:1352"
                     /country="Denmark"
     gene            complement(943..1356)
                     /locus_tag="SCJ86_002554"
     CDS             complement(943..1356)
                     /locus_tag="SCJ86_002554"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291922.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Hsp20/alpha crystallin family protein"
                     /protein_id="HEG1170064.1"
                     /translation="MANVPSIRDMFPDFNDVFSPAFNDFLGVSSYPKVDLVENEKEYK
                     LTADMPGCDKEDTTVEYSDNTLTISANHESHTEDKEDGNYVRKERHSVSYKRSFYLPN
                     VDEEKITGTFKNGVLKLVLPKTAYQPKETKKIELN"
     gene            complement(1812..2297)
                     /locus_tag="SCJ86_002555"
     CDS             complement(1812..2297)
                     /locus_tag="SCJ86_002555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303864.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEG1170065.1"
                     /translation="MHKGIYAGLLGTVILFSACGYKTKNSVKESSAAISTTEVGSTKS
                     TSTTSITNTSTGNVDSQHSQSSIQSFSISSTSEMQESTVPSLPLENSDKALLFLIAHR
                     SELQSDDIVISFYQKIDRDYLFTASSKQIRSQGGSGSVGFYRVSPEGSITMTDANGTP
                     F"
     gene            complement(2436..2651)
                     /locus_tag="SCJ86_002556"
     CDS             complement(2436..2651)
                     /locus_tag="SCJ86_002556"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345951.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF378 domain-containing protein"
                     /protein_id="HEG1170066.1"
                     /translation="MKTLDAIALTLLIVGGLNWLLVGLFELDLVAMIAGGSTTIFAKI
                     IYIVVGICAIYCLKFFPMITRKVDERY"
     gene            2875..3675
                     /locus_tag="SCJ86_002557"
     CDS             2875..3675
                     /locus_tag="SCJ86_002557"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631871.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HEG1170067.1"
                     /translation="MSDWLDIAGKVVIVTGGSSGIGRSIVENLLKQNAQVANFDVTEC
                     RIQHENLLSLKVDVSSKTDIKEGIYKVMKHFKTIDGLVNNAGINIPSLLIDRNHPKSK
                     YELSEQVFDKMIAVNQKSVYLMSQAVGRILVQKGSGVIVNLSSESGLEGSEGQSCYAA
                     TKAAMNSFTRSWAKELGKRNVRVVGVAPGILEETGLRTQEYEEALSYTRGISVEQLRN
                     GYSRTSTIPLGRSGKLQEVADLVCYYLSERSSYITGVTTNISGGKTRG"
     gene            3694..5562
                     /locus_tag="SCJ86_002558"
     CDS             3694..5562
                     /locus_tag="SCJ86_002558"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH domain-containing protein"
                     /protein_id="HEG1170068.1"
                     /translation="MTLVNRWYQLLKLLVTHKKVSIQEVQKEIKTSNQTIKKDIGELN
                     EEIAGIAKIIQKNKHFELEIYDFDFFDEIMQGKLKTASDFNSASKRIAYLLNRLIETE
                     DFLRMDDLSEEVGVSRGTVVKDLKTLKEMINDFDVKITGTPNKGLRIIGDELELRLLL
                     FYFVSPYFSETKTEQFLEEKQAIKHFQKKTNASKQEITLLTKVIGISLNRISSNYLLK
                     KPINRYSGCFEYIQEFNELINQLEEIYELTLSQYEKDFISFPLNIINTSITMIRKANS
                     DLRLYFDPMMMRIRRLDLIDLDESFLYQEMKQHLSLLINRVIFRYRLTDLFYGEIEKK
                     YPFSYQIASECIQALEAILQHKISKVEISYLTLYLELAFRYQKEHFSSKKVAIVHTTG
                     KGTALMIQRQIKRILGRDIQITHVSEEQYEKVDLNKYFAVFTTIPLKNVNEETPVIHL
                     TNLFDDSWLRAQWQKAKKKRLAGFHSTVFFYQIWKEKKTYQEYLNKLVAPLIRGYYVD
                     EEFEKRILKREYQQSTFVNKEFAFPHTTNQLSQHIVLSVGLITDQGLAETGGNGVIFL
                     FAIPEEMTAEVENELFELYDILFELIKMGIDEFIELAPTQESFLKLIKSKGERLHE"
     gene            5555..6046
                     /locus_tag="SCJ86_002559"
     CDS             5555..6046
                     /locus_tag="SCJ86_002559"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002390786.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR family transcriptional regulator"
                     /protein_id="HEG1170069.1"
                     /translation="MNDMYLFIVFAASAYVLQAVISWKQLKHFNQTYSELRRKGKVAI
                     GKRPGKIRSGTIVMLAIDDHDQVLEVRKMQGVTSLAKFHSLPVYAGEDIHYIDRYHPS
                     VRAEDRLTQAALENAREVYLRIEAGNEKSEQPSLPFIAMLNNFRIWKSTIKEKRSDKR
                     WIS"
     gene            6034..6588
                     /gene="srlA"
                     /locus_tag="SCJ86_002560"
     CDS             6034..6588
                     /gene="srlA"
                     /locus_tag="SCJ86_002560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327877.1"
                     /note="catalyzes the phosphorylation of incoming sugar
                     substrates along with their translocation across the cell
                     membrane; the IIC domain forms the PTS system
                     translocation channel and contains the specific
                     substrate-binding site; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucitol/sorbitol transporter subunit IIC"
                     /protein_id="HEG1170070.1"
                     /translation="MDIITKFAEGFMRLFQTGAETFISWMTGIVPVVLMLMVAMNTLI
                     AFLGEDRVTKVAKLSAKNPLTRYLVLPFLSAFMLGNPMSFTMARFLPEYYKPSYYAAQ
                     AQFCHTSNGVFPHINPGELFVWMGIAQGIETLGLNSMELAIRYLLVGLLMNFIGGWIT
                     DFTTGFVCRQQGIVLSKKVELSVD"
     gene            6606..7601
                     /locus_tag="SCJ86_002561"
     CDS             6606..7601
                     /locus_tag="SCJ86_002561"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299265.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucitol/sorbitol transporter subunit IIB"
                     /protein_id="HEG1170071.1"
                     /translation="MYGDIKIVRGNGGYGGPLIIRPTKDKHKFIYVTGGGERPMIVDK
                     IEELTGMESINGFKTSIPDSEIALAIVDCGGTLRCGIYPKKGIPTINIVPTGKSGPLA
                     QYITEDIYVSAVGLEQIQPLMETDDNKETVNTASPDKKYKYDTSKKITEQKAEQQNWI
                     AKIGMGAGKVVATFNQSAREAVDTMIRTVIPFMAFVSLLIGVIQGSGIGNVFANIMAP
                     LAGNAVGLTIIGFICSLPFLSPLLGPGAVISQVIGTLIGVEIGKGNIPPQLALPALFA
                     INTQNACDFIPVGLGLEEAKAETVEVGVPSVLYSRFLNGIPRVIIAWVASFGLYS"
BASE COUNT         2709 a         1213 c         1442 g         2237 t
ORIGIN      
        1 tttgttacta tgaataacat aataagaaag taaagtggat tgatttgaga tctttttata
       61 cttcaaaaaa gataacaccc aactttcaaa aatatgtttt ggaagttggg acaaacgagt
      121 taatacattt taatacatta ttttttattc aaaaaaaata gcacaatggc aaagatcgta
      181 agagagatca taagccccaa tgaatgacta aaaagaaaag cagaatcaca tgccatatca
      241 tcccatattt cttcaagaaa ataaataagc gggaaaaagg gaaggaatag tagaacatcg
      301 atccagcacc atttttttct tcttttgaaa taacataaaa caataaacat gaacgtgcag
      361 ggaagtaaaa acaaagcttt actcataact aacaaaatac cagctccttt tatttttttc
      421 tcccccacat tcaaagagta acaatggtat gtacttaata atagaaaaat ggatcaactt
      481 gtatgaataa cagctgaaaa gatctgcacc aaataaaaaa cttccagcca aaactgactg
      541 gaagtttatt tttttctgaa atattctaac acaaatatga aaaagatggc cgaagctaat
      601 aagccgattt cgtagcaaaa caatgagata ccagagtcgt aataataaaa tcctctaatc
      661 ttatggataa aatagatcat cggacaagca gataagagta agtatttgtt aggccttttg
      721 aataagtaaa agaaagcact ggtcattatg ataaatggta tccaaaacaa aagtttactt
      781 aagacaatca aatgatcaac tcccatattt tttaccaaga gagatcataa catctttatc
      841 gtaaaaacaa aatatttttt tgctcatttt gaaaatttaa acatactcgt tcttatccaa
      901 atatataaaa atcgcccctc attcatcgag aggcgataag ttctaattta actcaatttt
      961 ttttgtttct tttggctgat aagctgtttt aggtaatacc aatttcagca caccgttttt
     1021 aaaggttcca gtgatttttt cttcatcaac attaggaaga tagaatgaac gtttgtaaga
     1081 gaccgaatgt ctttctttcc gtacatagtt gccatcttct ttatcttctg tatgggactc
     1141 atgattggcg gagatcgtca aagtattatc tgaatattca actgtcgtgt cttctttgtc
     1201 acagccaggc atatcagcag ttagtttgta ttctttttca ttttcgacta aatctacttt
     1261 tggataactt gaaacaccta aaaaatcatt gaatgctgga ctaaacacgt cattgaaatc
     1321 agggaacatg tcgcgaatgc taggtacatt tgccattttt gatcatcctt tcaataagta
     1381 ttttttattt atccctattt acgtaataac tctattttga aaaaaatgca agtataatgg
     1441 ttagctttgt gaatatttca tctaatacga tacaaaggct atctttctaa tttgaaaaag
     1501 ggatcattaa aataataaga agaaaatatg tcaaaaggta aaaaagtaaa ttcttctatc
     1561 caaatataga aaaattgaat ctagaaaacc gcccgacaat aaatttcatt aactcagttg
     1621 accgaaaatg aagtagatac caaaaattta tcaagagagc aagtagagaa gtgagtatat
     1681 tcataagaga aatagctgta acgaattatt aggttaatgc aaatgcttat ttatactccg
     1741 tataactttc tcagaaaaaa tgataccaat aatgacttgc tagtactgta aatccgtata
     1801 atatcatcta atcaaaatgg tgttccattt gcatctgtca tcgtaatcga tccttcgggt
     1861 gacactcgat aaaaaccaac gcttccagat cctccttggg aacggatttg tttgctggaa
     1921 gctgtgaaca aataatctcg atcaattttt tgataaaagc taataacaat atcgtcactt
     1981 tgtaattcgg aacggtgggc aataagaaat agaagtgctt tgtcagagtt ttctaatgga
     2041 agagaaggta cagtgctttc ttgcatttca gatgttgaag aaatgctaaa cgactgaata
     2101 cttgattggc tatgttgcga atctacatta ccagtagaag tattagtaat ggaagtagta
     2161 gaagtacttt tagttgaacc aacctcagtt gttgaaatgg ctgctgaaga ttctttaaca
     2221 gaattctttg ttttatatcc acaggcagaa aaaagtatta cagttcccaa caagcctgcg
     2281 taaatccctt tatgcatgat agctcctcct aaaatagatt atctttattc agtatatcac
     2341 ttaaacaaag aaaagcagat ggtttaaaat gaaaaaacct tcccaataaa gggaaggttc
     2401 atgaagataa taactttttt aataattata gctttctaat atctttcatc aacttttcta
     2461 gtaatcattg ggaaaaattt caagcagtag atcgcacaaa tacctacgac gatataaatg
     2521 atcttcgcaa aaatagttgt tgaaccacca gcaatcatag ctactaaatc aagttcgaac
     2581 aagcctacca acagccagtt caaccctccc acaatcaata aagttaatgc aatagcatct
     2641 aaagttttca ttagaattct cctttcttaa atgataaaac aattatatat caaaaagaga
     2701 aacattgctt atataacgct caggaatttt agcaatctat gcaaaaagat gaaaattttt
     2761 tttgctcttc cattagtaca cattttttag ctgaagctgt aaatttcatg tcttaatttt
     2821 ttttagtgag cttttatact ggatttgtaa gaaaaaagaa ggagaggatc acatatgtcg
     2881 gattggttgg atattgcagg aaaagtagta attgtaactg gaggttcctc aggaattgga
     2941 agaagtatcg tcgaaaattt gttgaagcag aatgcacaag tagcgaattt tgatgtaact
     3001 gaatgccgta tacagcatga aaacctacta tctttaaaag tcgatgtgtc ttctaaaacg
     3061 gacataaaag aagggatata taaagtaatg aaacatttta agacgatcga tggtctagtc
     3121 aataatgcag gaataaatat tccttctctt ctaatagata gaaatcatcc aaaaagcaag
     3181 tatgaattat cagaacaagt ttttgataag atgatcgctg tcaatcaaaa aagtgtatat
     3241 ttgatgagcc aagcagttgg aagaatcttg gtgcaaaagg gatcaggagt gatcgtgaat
     3301 ctttcttctg aatcaggatt ggaaggatca gaaggacaga gctgttatgc tgctacaaaa
     3361 gcagcgatga acagttttac tcgttcttgg gcaaaagagt taggaaagag gaatgttcgt
     3421 gtggtaggtg ttgcaccagg gatactggaa gaaaccggtt tgagaacaca agaatatgaa
     3481 gaagctcttt cttatacgag aggtataagc gtcgaacagc tgagaaacgg ttacagtcga
     3541 acaagtacga tccctctagg aaggagtggt aagctgcaag aagtggcgga tctagtctgc
     3601 tattatcttt cagaaagatc aagttatatc actggagtca cgactaatat ttcaggaggt
     3661 aaaacaagag gataaaggat aggggggacg cacgtgacgc tagtaaacag atggtaccaa
     3721 ttattgaaat tactagtcac tcacaagaaa gtttcaatcc aggaagtgca aaaagaaatc
     3781 aaaaccagta atcaaaccat aaaaaaagat attggggagt tgaatgaaga aattgccggt
     3841 attgcaaaaa tcatccaaaa aaacaaacat tttgaacttg aaatttatga ttttgatttt
     3901 tttgatgaga tcatgcaagg aaaattgaaa acagccagtg atttcaattc ggcatccaaa
     3961 agaatcgctt atttactaaa taggctgatc gaaacagagg atttcttacg tatggatgat
     4021 ttgtctgaag aagtcggtgt cagcagagga acagtggtca aggatctaaa gacattaaaa
     4081 gagatgatca atgattttga tgtaaagatt acaggcacac caaataaagg actaagaata
     4141 ataggagatg agctagaatt acgactactt ttattttatt ttgtttctcc ctacttttca
     4201 gagacgaaaa cagaacagtt tctagaagaa aaacaagcaa tcaaacattt tcagaagaag
     4261 acgaatgcta gtaaacaaga aatcaccctt ttaacgaaag ttatcggtat ttctttgaat
     4321 agaatctcta gtaactatct gttgaagaag cccatcaatc gttacagtgg gtgttttgaa
     4381 tatatccaag aattcaatga actgatcaat cagttggaag aaatctatga actaactttg
     4441 agccaatatg aaaaagattt tatcagtttt cctttgaaca tcatcaacac aagtataacg
     4501 atgataagga aagctaactc tgatttgcgc ttatatttcg accccatgat gatgcgtatt
     4561 cgacgattag atctcattga tctggatgag tccttcttat atcaagagat gaaacagcac
     4621 ctctctttat tgataaatcg ggtaattttt cgttatcgat tgactgattt attttatggt
     4681 gagatagaaa aaaagtatcc tttttcttac caaatcgcta gcgaatgcat acaagcatta
     4741 gaagctatct tgcaacataa gatctctaaa gtagagatca gctacctaac gttgtatttg
     4801 gagttagcat ttcgatatca aaaagagcat ttttcctcta aaaaagtagc cattgttcat
     4861 acgacaggaa aaggaacagc attaatgatc caaagacaaa tcaaaaggat attggggagg
     4921 gatatacaaa taacacatgt ttcagaagaa caatatgaaa aagtagatct aaacaaatat
     4981 ttcgctgttt ttacaacgat tccgttaaaa aatgtaaatg aagagacacc ggtgatccat
     5041 ttgactaatc tttttgatga ctcttggtta agggcacaat ggcaaaaagc aaaaaaaaag
     5101 cgattagccg ggtttcattc tactgttttt ttctatcaaa tatggaaaga aaaaaagacg
     5161 tatcaagaat atttaaacaa attagtcgct cctttgatca gaggctatta tgtggatgaa
     5221 gaatttgaaa aaaggatctt gaaaagagaa tatcagcaaa gcacatttgt taacaaagaa
     5281 tttgcttttc ctcacacaac caaccagcta tctcaacaca ttgttttatc agtagggcta
     5341 ataactgatc aaggtttagc agaaacagga ggaaatggag ttattttttt atttgccata
     5401 cctgaagaaa tgacggctga agttgagaat gaattatttg agctatacga tatcctattt
     5461 gaactcataa aaatgggaat agatgagttt attgaattag caccgacaca agaatcattt
     5521 ttaaaactaa tcaaaagtaa aggagagaga cttcatgaat gatatgtatc tattcatagt
     5581 tttcgcagct tcagcttatg tgctgcaagc agttatcagt tggaagcaac taaagcattt
     5641 caatcaaaca tatagcgaac tgcgaagaaa aggaaaagta gcgattggta aacgacctgg
     5701 gaaaatccgt tcaggcacga ttgttatgct agctatagac gatcatgatc aagttttaga
     5761 agtcagaaaa atgcaagggg ttacatcttt ggcaaaattt cattcattac ctgtctatgc
     5821 gggtgaagat atccattaca tcgatcgtta ccatccctca gttcgagcgg aagatcgttt
     5881 gacacaagca gctttagaaa atgccagaga agtatactta cgaatagagg caggaaatga
     5941 aaaaagtgaa caaccttcat tgccttttat tgctatgctc aataattttc ggatatggaa
     6001 aagtacgatc aaagaaaaaa ggagtgacaa acgatggata tcataacaaa atttgctgag
     6061 ggatttatga ggctgttcca aacaggtgca gaaacattca taagctggat gacaggaatc
     6121 gtaccagttg tcttgatgct gatggttgca atgaatacgc tgattgcttt tttaggtgaa
     6181 gaccgagtaa cgaaagtagc caaattatca gcgaaaaatc cgcttactcg ttaccttgtg
     6241 ttaccgttct tatctgcttt tatgttggga aatcctatgt catttacaat ggctcgtttc
     6301 cttccagaat actacaaacc aagctattat gctgctcaag ctcaattttg tcatacgagc
     6361 aatggtgtat tcccccatat taatcctgga gaattgtttg tatggatggg gattgctcaa
     6421 gggatcgaaa ccttagggct aaattcgatg gagctggcta tccgatactt gcttgtggga
     6481 ctgctgatga actttattgg tggttggata actgatttta ctactggatt tgtatgcaga
     6541 cagcaaggaa tcgttcttag caaaaaagta gagctttcag ttgattagag aaaggagagt
     6601 tttaaatgta tggtgatatt aagatcgtaa gaggaaacgg cgggtatgga ggtcctttga
     6661 ttattaggcc gacaaaagat aaacacaagt tcatctatgt cacaggtgga ggtgagaggc
     6721 caatgatcgt agataaaata gaagaactga caggtatgga gtctatcaat gggttcaaaa
     6781 catcaatacc tgatagtgaa atcgcgcttg caatcgttga ctgtggaggg actcttagat
     6841 gtggaattta tcctaaaaaa gggataccaa ccatcaatat cgttcccaca ggaaaaagtg
     6901 gcccattagc acaatatatc actgaagata tttatgtttc agcagtaggg ttagaacaaa
     6961 tacagccact aatggaaact gatgataaca aagaaacagt aaatactgca tctccagata
     7021 aaaagtacaa atatgatact tctaaaaaga taaccgagca aaaagcagag cagcaaaact
     7081 ggatagcaaa aattggaatg ggcgcgggaa aagtagttgc tacattcaat caatcagcaa
     7141 gagaagcagt agatacgatg atccggacag tgatcccttt tatggctttc gtttcccttt
     7201 tgattggtgt gatacaaggt tctgggatcg gtaatgtatt tgctaacatt atggcacctt
     7261 tagcaggaaa tgcagttgga ttaacaatca ttggctttat ttgttcactg ccatttttat
     7321 ctcctttgct tggtccaggg gcagttatta gccaagtgat tggaacattg atcggtgtag
     7381 agattgggaa aggaaatatt cctccccaat tagcattacc ggcactattt gctatcaata
     7441 ctcaaaatgc atgtgatttt attccagtcg gtctaggttt ggaagaagca aaagctgaga
     7501 cagtagaagt aggtgtacca tcagttcttt attcaagatt tcttaacggc atcccacgag
     7561 ttatcattgc ttgggtagca agttttggat tatatagctg a
//
LOCUS       DARBET010000101         7377 bp    DNA     linear   BCT 07-NOV-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4027
            SAMEA114569841-rid20112183.denovo.105, whole genome shotgun
            sequence.
ACCESSION   DARBET010000101 DARBET010000000
VERSION     DARBET010000101.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA114569841
            Sequence Read Archive: ERR12203666
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 7377)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7377)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001972618.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001972618.1
            Genome Coverage       :: 132x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/07/2023 13:18:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,041
            CDSs (total)                      :: 2,975
            Genes (coding)                    :: 2,908
            CDSs (with protein)               :: 2,908
            Genes (RNA)                       :: 66
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 67
            CDSs (without protein)            :: 67
            Pseudo Genes (ambiguous residues) :: 0 of 67
            Pseudo Genes (frameshifted)       :: 26 of 67
            Pseudo Genes (incomplete)         :: 28 of 67
            Pseudo Genes (internal stop)      :: 20 of 67
            Pseudo Genes (multiple problems)  :: 6 of 67
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7377
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA114569841-rid20112183.denovo.105"
                     /isolate="VRE4027"
                     /db_xref="taxon:1352"
                     /country="Denmark"
     gene            123..515
                     /locus_tag="SCJ86_002562"
     CDS             123..515
                     /locus_tag="SCJ86_002562"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEG1170072.1"
                     /translation="MLDDRLTCSNCGTLFYYEEVRNIVKHRKKEMPIRCPNCNEVIKR
                     KLSHGYFVSYELYEESKINLKKKPKYYKNLKTGAIITEKEFKNLVTEEESRFYNLSGN
                     ICPGFMDQIIENESIESIENYVPVDLPK"
     gene            complement(688..870)
                     /locus_tag="SCJ86_002563"
                     /pseudo
     CDS             complement(688..870)
                     /locus_tag="SCJ86_002563"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324322.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS256 family transposase"
     gene            857..1054
                     /locus_tag="SCJ86_002564"
     CDS             857..1054
                     /locus_tag="SCJ86_002564"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEG1170073.1"
                     /translation="MKDSIVEPEGMENTFELYNKTTVDKLIPILVPTDLNGHDVLLRF
                     NGFLGEDYRYLKISDPQPLTE"
     gene            complement(1097..1672)
                     /locus_tag="SCJ86_002565"
     CDS             complement(1097..1672)
                     /locus_tag="SCJ86_002565"
                     /EC_number="2.7.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002984668.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thymidine kinase"
                     /protein_id="HEG1170074.1"
                     /translation="MAQLFYRYGTMNSGKSIELLKVAHNYEEQGKRVIIMTSAIDTRS
                     GFGKVESRIGVSKPAIAITSEMDVYGELVEIIDQYGKVDCILVDESQFLSRENIIDLS
                     LIVMNEEVPVICYGLKNDFQNNLFKGSEALLTFADKIEEIKTICHFCNKKAVMNARIV
                     DGIPVYSGEQIVVGGNEDYLSVCRNHYYNED"
     gene            complement(1827..2353)
                     /locus_tag="SCJ86_002566"
                     /pseudo
     CDS             complement(1827..2353)
                     /locus_tag="SCJ86_002566"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324322.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            2810..4321
                     /gene="gadC"
                     /locus_tag="SCJ86_002567"
     CDS             2810..4321
                     /gene="gadC"
                     /locus_tag="SCJ86_002567"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015082498.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate:gamma-aminobutyrate antiporter"
                     /protein_id="HEG1170075.1"
                     /translation="MNKKKLSLFGFFALTASMVLTVYEYPTFATSQLHLVFFLLLGGI
                     LWFLPVALCAAEMATVEGWKDGGIFSWVSQTLGERFGFAAIFFQWFQITVGFITMIYF
                     ILGALSYVINFPELNTDPFIKFIGVLVIFWVLTFSQLGGTNRTAKIAKAGFVIGIVIP
                     SIILFGLAAAYFIGGNPIQMAINAKAFIPDFSQLSTLVVFVSFILAYMGVEASASHIN
                     ELENPQRNYPLAMFLLVILAIALDAIGGFSVAAVIPQHDLSLSAGVIQTFETLILHFN
                     SHLHWLVKVIALMIACGVMGEVSSWVVGPSRGMFAAAQRGLLPVFFRKTNKQDVPVPL
                     IMIQGIIVTIWAAVLTFGGGGNNLSFLVAISLTVVIYLVGYLLFFLGYFVLVFKKKDL
                     KRTYNVPGKTVGKTIIGGIGFILSVFALLISFVPPASIAASEHRTYQIILLISFVVTV
                     LLPFIIYEVHDKSAHTTIEEPTHVKAEDVNPAVYPAARGEHHVTKNDEHILKH"
     gene            4334..5734
                     /locus_tag="SCJ86_002568"
     CDS             4334..5734
                     /locus_tag="SCJ86_002568"
                     /EC_number="4.1.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287852.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate decarboxylase"
                     /protein_id="HEG1170076.1"
                     /translation="MLYGKDNQEEKNYLEPIFGSASEDVDLPKYKLNKESIEPRIAYQ
                     LVQDEMLDEGNARLNLATFCQTYMEPEAVKLMTQTLEKNAIDKSEYPRTTEIENRCVN
                     MIADLWHAPNNEKFMGTSTIGSSEACMLGGMAMKFAWRKRAEKLGLDIQAKKPNLVIS
                     SGYQVCWEKFCVYWDVELREVPMDEKHMSINLDTVMDYVDEYTIGIVGIMGITYTGRY
                     DDIKGLNDLVEAHNKQTDYKVYIHVDAASGGFYAPFTEPDLVWDFQLKNVISINSSGH
                     KYGLVYPGVGWVLWRDQQYLPEELVFKVSYLGGEMPTMAINFSHSAAQLIGQYYNFVR
                     YGFDGYRDIHQRTHDVAVYLAKEIEKTGIFEIINDGSELPVVCYKLKEDPNREWTLYD
                     LSDRLLMKGWQVPAYPLPKDLDQLIIQRLVVRADFGMNMAGDYVQDMNQAIEELNKAH
                     IVYHKKQDVKTYGFTH"
     gene            complement(5899..7339)
                     /locus_tag="SCJ86_002569"
                     /pseudo
     CDS             complement(5899..7339)
                     /locus_tag="SCJ86_002569"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328084.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein"
BASE COUNT         2329 a         1224 c         1311 g         2513 t
ORIGIN      
        1 attttttctt ttaagtaaaa aatataggtg attttttttg acttcatatt tataataaag
       61 acacgtaaat caattatttt tcattttact ttaactaata atgtttattt ggagggatta
      121 ttatgttaga tgatagattg acgtgttcta attgtggtac attattttac tatgaggaag
      181 ttagaaatat tgtaaaacat agaaaaaaag aaatgcctat acgttgtcca aattgcaatg
      241 aagttattaa gcgaaagttg tctcatggtt attttgtcag ctacgaacta tatgaagaaa
      301 gtaaaataaa tctgaaaaaa aaaccaaaat actataaaaa tttgaaaact ggagctatta
      361 taactgaaaa agaatttaaa aatttagtga ctgaagaaga aagcaggttt tataatttgt
      421 ctggtaatat ttgccctgga tttatggatc agattattga aaatgagtca attgaatcaa
      481 ttgaaaacta tgttcctgtt gacttaccaa aatagaaaaa ataatcctgt ttttacagtg
      541 aattatttta attttgattt tcaataaaaa tatattacaa taataatcgt aatagctaat
      601 gaaacaattt ctcaatcttt ttaccactcc aaacagggag cgtcaataat tttgtgtaaa
      661 taactcgtcc ttctgcaaaa taatgggtta ctcagtaaac attgaagcta atgtatccgt
      721 cacttgttgg aagcctttat ggcttctatt taagaatttt tgattgtatg tatcaaagat
      781 ggatactagg aaacgctcta gtgattcttc attttgaaac tgctcttttc tgcggctgta
      841 tttcttgatt tgtttattga aagattcgat tgtagaacca gaaggaatgg agaacacttt
      901 tgaactttac aacaaaacta ctgttgataa attaattcca attttagttc caactgattt
      961 aaatggtcat gatgttttac ttcgttttaa cggattttta ggtgaggatt atagatacct
     1021 aaaaatttct gatcctcaac ctttaacaga ataataaaaa acgtgtaatc aattatttga
     1081 ttacacgttt tttatttcaa tcttcgttgt agtagtggtt gcgacatacc gaaaggtaat
     1141 cttcattacc tcctactaca atctgttcac ctgaataaac tggaatacca tcaacaatac
     1201 gagcattcat tacagccttt ttattgcaaa aatggcaaat agttttaatt tcctcaattt
     1261 tatcagcaaa tgtcaataat gcttcggatc ccttgaagag attattttga aaatcattct
     1321 tcaatccata gcaaattact ggtacttcct cattcattac gataagtgat agatcaataa
     1381 tattctctct acttaaaaat tgagactcat cgaccaaaat acaatcaact tttccgtact
     1441 gatcaataat ctccactaat tctccgtaca catccatttc agaagtaatt gcaatagctg
     1501 gtttcgaaac acctatacga ctctcaactt ttccaaatcc tgatcgagta tctattgcgc
     1561 ttgtcattat gataactctc ttcccttgtt cttcataatt atgagccact ttcagtagct
     1621 caatactctt tccactattc attgttccat atctgtaaaa taattgtgcc attaaattta
     1681 ttcccttctt ttacaattca attattcctt aattccatct gcttttatgc agctgctcgt
     1741 ttattccttg atatgtctat aacatctact atccctaaaa ccaatttata agccattaga
     1801 gatggtattc ctgaacatct ctcatattgt tttttgagtg gtatatcatc tgaaaaactg
     1861 aaggaatcat gaaaatagcg acatacttat catttagagt tctaacttta aatgcattta
     1921 cttcttcagt cagcacttta gtcatgttgg acatggtttg gggcgtgtag tgatgcccat
     1981 acattttctc aatcaagtct gcgatttccg acatggtaat accttttcgg aagaggtgaa
     2041 tgacggtctc ctctaatgtg tcattcgtcc gtctataagc aggaacagtc tgttgcttga
     2101 actcgccatt gcggtcgcgc ggaatctgga gatgaagttc cccatactcg gtcttgaccg
     2161 tacggtcata ggagccgtta cgagaattac ccgtgttaaa accaatgcga tcgtactttt
     2221 cgtaatcgag gaaagccgtt aactccgttt tgagaagcgt attgacagct ttttccaaat
     2281 ggacacggaa taatccattc aaatcgcctt tgttcgctag agtctttagg atttctgtag
     2341 taaaatcgtt catatggaag tcctcttttc tgtgaatgtg ttgtggtaac tctattctac
     2401 agaagggact tccttttttc tatttacaca aaatatttta cactctcaac aaaagtatct
     2461 tcgattaata cttgctcaag ttatgtgcag ctacgaaaaa gtaggtaccg aggtactttc
     2521 tttttcgcag ctttttttgg acttatgaga agtacattcg ctggaagaat acgatataaa
     2581 acgagctttt tcactaaaga tattaataga gtgaaagaaa caagcttttg ttgtcagttt
     2641 tttcactttt ctaaaaatga aaataaaaaa ttctggaagt ggagaagaat tttgcttgct
     2701 atactttgtc ttgtagaaaa gaggtagaca aaataatgca agctcaaata agcgattttc
     2761 cataaccttg gtaggaatga caaattaaat aggaaaggga agtaataata tgaataaaaa
     2821 aaagttatct ctattcggtt tttttgccct gacagcttcc atggttttga ctgtttatga
     2881 gtatccgacc tttgcaacat cacaattaca tttggttttc tttcttcttt tgggaggtat
     2941 tttatggttt ctaccagttg ctttatgtgc tgcagaaatg gcaacagttg aaggatggaa
     3001 agatggtggg attttcagtt gggttagtca aactttagga gaacgttttg gttttgcagc
     3061 tatttttttc caatggtttc aaattacagt aggttttatt acaatgatct acttcatact
     3121 aggagcgctg tcgtatgtaa ttaattttcc agagcttaat acagatccat ttatcaagtt
     3181 tattggtgta ttagtcattt tctgggtact gactttttca cagttgggcg ggaccaatcg
     3241 taccgctaaa attgctaaag caggatttgt cattggaatt gttattcctt cgatcattct
     3301 ctttggttta gcagcagcct actttatcgg cggtaatccg attcaaatgg ctattaatgc
     3361 gaaagctttt attcctgact tttctcagct ttcaacttta gtagtatttg tatcttttat
     3421 attggcttat atgggcgtag aagcttcagc atcacatatt aatgagctgg aaaatcccca
     3481 aagaaactac ccattggcaa tgtttttact agtgattttg gccatcgcct tagatgcaat
     3541 cggcggtttc tcagtagcag cggttattcc acaacatgac ttgtctctca gtgctggtgt
     3601 aatccaaaca tttgaaacgc ttattttaca ttttaacagt catttacatt ggttggtaaa
     3661 agtgattgct ctcatgattg cctgtggcgt tatgggtgaa gttagttctt gggtagttgg
     3721 tccatcacga gggatgtttg cagctgctca gcgaggtctg ttaccagttt tcttccggaa
     3781 aactaataaa caagacgttc cagttccgtt gattatgata caaggaatta tcgttactat
     3841 ttgggcagca gtcttaactt ttggtggagg aggcaacaat ttatcattct tagtagcaat
     3901 ctccttaact gttgtcattt atctcgtagg atatttacta tttttcctag gttactttgt
     3961 attagttttt aagaaaaaag acttaaaacg aacttacaat gtaccaggaa aaactgttgg
     4021 caaaacgatt atcggtggaa ttggttttat tttatcagtg ttcgcattgc ttatctcttt
     4081 tgtaccacca gcttcaattg cagctagtga gcaccgaaca taccaaatca tactacttat
     4141 tagctttgta gtgactgttc ttttaccttt tatcatttat gaagtacatg ataaaagtgc
     4201 tcacacaact attgaggaac ctactcacgt gaaagctgaa gacgttaatc ccgctgttta
     4261 tccagctgct agaggagagc accacgttac aaaaaatgat gaacatattt taaaacatta
     4321 ggaggaattc attatgttat acggaaaaga taatcaagaa gaaaaaaact atttggaacc
     4381 aatttttggc tctgcaagtg aggatgttga cttgcctaaa tataagttaa acaaagaatc
     4441 cattgaacca cgaattgctt atcaattagt acaagacgag atgttggatg aaggaaatgc
     4501 gcgattaaac ttagctactt tttgtcaaac gtatatggaa cctgaagcag tgaaattgat
     4561 gacccaaacg ttagaaaaaa atgcaattga taaatcagaa tacccacgaa caacggaaat
     4621 tgaaaaccgc tgtgtaaata tgattgctga tttatggcat gctccaaata atgaaaaatt
     4681 catgggaact tcaacgatcg gctcttcaga agcctgcatg ctgggtggta tggccatgaa
     4741 atttgcttgg cgtaaacgtg ctgaaaaatt aggtcttgat attcaagcaa aaaaacctaa
     4801 cctggtgatc tcttctggtt accaagtttg ttgggaaaaa ttctgtgtat attgggatgt
     4861 ggaactgaga gaagtcccaa tggatgaaaa acatatgtca attaatctag atactgtcat
     4921 ggattatgtg gatgagtaca caattggtat tgtaggtatt atgggtatta cttacactgg
     4981 tcgttatgat gatatcaagg gtctgaatga tttagttgaa gctcacaata aacaaactga
     5041 ctataaagta tacattcatg ttgacgctgc atcgggtggc ttttatgcac catttactga
     5101 acctgatcta gtttgggatt ttcaattgaa aaatgttatc tcaattaatt cttcaggtca
     5161 caaatatggt ttggtatatc caggtgtggg ttgggtctta tggcgtgacc aacaatactt
     5221 accagaagaa ttagtattta aagttagtta cttaggtgga gaaatgccaa ctatggctat
     5281 caacttctct catagtgcag cacaactaat tgggcaatac tacaactttg tacgctatgg
     5341 ctttgatggt tatcgtgata ttcaccaaag aactcatgat gttgctgttt atttagccaa
     5401 agagatcgaa aaaactggta tttttgaaat cattaatgat ggatcagaat tgccagttgt
     5461 gtgctataag ctgaaagaag atcccaatcg cgaatggaca ctatatgatt tatctgatcg
     5521 tctgttaatg aagggatggc aagtcccagc ctacccatta cctaaagact tggatcaatt
     5581 aattattcaa cgcttagttg ttcgagcaga ctttggaatg aacatggctg gtgattatgt
     5641 acaagatatg aaccaagcaa ttgaagagtt gaataaagct catattgttt atcataaaaa
     5701 acaggatgtg aaaacgtacg gctttactca ctaaaattct tcagagaaat ataaaagaag
     5761 ttaattaact aataaaagga gttgtgatta ccacacagct ccttaaatct atttgaataa
     5821 atatatgata ttcatttgtt acactctcaa tcttagctgt tgttctattc atcattactc
     5881 ttattgtaag tagttcagtc aaatttgctt gttcaattga tcgataacag tatgaattgt
     5941 ttgaaaagta atcggatata taattgtttt ctcactacct aagccttctt ggtataaaga
     6001 actatcgcaa agtataatcc gctgagtaca aaagaaagtg tcgtctacta tttctctaag
     6061 ttgtttacaa atgatattac taatagaaaa gtgttctccc aaaatgtctt ctaactgttg
     6121 tcgatagtat aaatacctaa agtctgacgg agctactatg aacacttcaa ttggagattt
     6181 ccgtttatgc tcatttaaaa gacttaaact aatggttagt ttacgaatag aattctcatt
     6241 ccaacgcaga aataaattgt tttgttttcg ccattgttca agaatttcat gaacttctaa
     6301 gtacaaagcg cgttgctctt catttaacaa gtaagtctta tcaggttgaa atatctgaat
     6361 atcagcccaa agtgagcgga taaacgggag aagtgcttta tcaaaaagta tctgattttc
     6421 ttcagatata ttcagagata agataagttt ttgactgagc tcaatcacca aagggttatc
     6481 tgctacatgg ttttgataaa caacgttcaa gtctttttgt aacaattcaa agttatcgca
     6541 tagataattt ctagagttga atagaaagta gatataaagg cattcatctt cgtcatgaca
     6601 taaagagaaa gaaagttgct ctaatccttt ctgtattagc tgaaacaagg gagtatgtcg
     6661 ggcaactctt ttttcttcct gactaaaata tattgggtta gtcttagatc tctgcaaacc
     6721 gatagcgata cctaataaaa taaattgttt ttcaactgta gggtaacttc ttgaaaaaaa
     6781 atgtttttct acataatcaa tcaattgttg accgctttga ataattgatt tatttagctg
     6841 gatcaactct gtccgatttg tctcatagat tatcgttagt aacaaacttc gataatcctt
     6901 ttcaggaatt tcaaaatggc catcagcatt taagtactgc atttgtttaa aaaaattaat
     6961 caactcattt cgtaagttgt aaactttgct taacgaaatg aaggtagtat cagctatatt
     7021 actccaattg tgttcccctg ataaataatg tcctagtaca ttgaaaaagt cagagcgctg
     7081 atagatgagt tgagtgagtg ccaacctcga aaacgaagag tcgaatgcga ctgtaaagct
     7141 acttctagat ttctccactg aaataatttg ctgatggagt atgtcggtaa tgcattccaa
     7201 gtcgttagtg acagacagtg agtgggcaaa cgttcaattg atctgctatt tcgcgaatct
     7261 gaatggtccc tttttcgtgt aattgctcac aaatgtaaac ttgacgaaaa atacttttct
     7321 ccagataggt ttccaacata gtactcctcc tagtcatttt atacataact attataa
//
LOCUS       DARBET010000102         7337 bp    DNA     linear   BCT 07-NOV-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4027
            SAMEA114569841-rid20112183.denovo.106, whole genome shotgun
            sequence.
ACCESSION   DARBET010000102 DARBET010000000
VERSION     DARBET010000102.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA114569841
            Sequence Read Archive: ERR12203666
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 7337)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7337)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001972618.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001972618.1
            Genome Coverage       :: 132x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/07/2023 13:18:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,041
            CDSs (total)                      :: 2,975
            Genes (coding)                    :: 2,908
            CDSs (with protein)               :: 2,908
            Genes (RNA)                       :: 66
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 67
            CDSs (without protein)            :: 67
            Pseudo Genes (ambiguous residues) :: 0 of 67
            Pseudo Genes (frameshifted)       :: 26 of 67
            Pseudo Genes (incomplete)         :: 28 of 67
            Pseudo Genes (internal stop)      :: 20 of 67
            Pseudo Genes (multiple problems)  :: 6 of 67
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7337
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA114569841-rid20112183.denovo.106"
                     /isolate="VRE4027"
                     /db_xref="taxon:1352"
                     /country="Denmark"
     gene            124..2733
                     /gene="clpB"
                     /locus_tag="SCJ86_002570"
     CDS             124..2733
                     /gene="clpB"
                     /locus_tag="SCJ86_002570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324026.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent chaperone ClpB"
                     /protein_id="HEG1170077.1"
                     /translation="MNIEKMTTTLQEAIAEAQQIAVTRKHQDIDIAHVWKIFLQSNHF
                     GRNFYTDAGLDVESFEHEIDRLLDEYPVVSGGNVQYGQNLSQNFFRLLNEADQIRESF
                     GDEFLSTEVVILALMKLKNYPLTVYLNKNGLSEKELRKNIEEMRGGERVTSQNQEEQY
                     KALEKYGVDLVQQVKSGKMDPIIGRDEEIRDVIRILSRKTKNNPVLIGEPGVGKTAIV
                     EGLAQRIVRKDVPENLKDKTIFSLDMGALIAGAKFRGEFEERLKAVLKEVKKSDGRIL
                     LFIDEIHNIVGAGKTEGSMDAGNLLKPMLARGELHLIGATTLDEYRQYMEKDKALERR
                     FQKVLVKEPTVEDTISILRGLKERFEIHHGVNIHDNALVAAATLSDRYITDRFLPDKA
                     IDLIDEASATIRVEMNSMPTELDQVTRRLMQLEIEEAALKKESDDASKKRLKNLQEEL
                     AELREEANAMKMQWETEKEEVNSVSAKRAEIDKAKHELEDAENNYDLERAAVLRHGTI
                     PQLEKELKELEAKAKDSEIKMVQESVTENEIAQVVGRLTGIPVTKLVEGEREKLIKLN
                     ETLHKRVIGQDEAVDAVSDAVIRSRAGLQDPNRPLGSFLFLGPTGVGKTELAKALAEN
                     LFDSEDHMVRIDMSEYMEKHAVSRLVGAPPGYVGYEEGGQLTEAVRRNPYTIVLLDEI
                     EKAHPDVFNILLQVLDDGRLTDSKGRVVDFKNTVLIMTSNIGSQLLLEGVTADGTIPE
                     AVAEQVNTLLRGNFKPEFLNRIDDTILFTPLSLDNVKGIVDKMVAQLAQRLEHQEILL
                     TISDEAKTWIAENAYEPAYGARPLKRFITKEVETPLAKEIVAGHVMPKSKVTITLLDG
                     QLHFKTEELEEIV"
     gene            complement(2884..3570)
                     /locus_tag="SCJ86_002571"
     CDS             complement(2884..3570)
                     /locus_tag="SCJ86_002571"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348404.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEG1170078.1"
                     /translation="MEGLSVIKEIKKVQIALLAFGVFVVCYNFYEFITQKYSTSQGIT
                     FIVESLLGIALIFMPQVILKVFKLKIPAAIVLFYWFFLFISVFLGTGMHLISIISFWD
                     KILHAVSPMVLTALGYGLIGYLMKDAEISKTSPWLFLLFGFAFAGLCGVFWEFWEFLC
                     DQFLGMNLQRFAASDGTLFVGRAALMDTMGDLLTNTIGAALMGLFAWSQSKKDERYFE
                     SYKLEKVKNT"
     gene            complement(3548..4042)
                     /locus_tag="SCJ86_002572"
     CDS             complement(3548..4042)
                     /locus_tag="SCJ86_002572"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288352.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside deoxyribosyltransferase"
                     /protein_id="HEG1170079.1"
                     /translation="MKIYFAAPMFAKSDLLYNQQLVKEIRELSSELSIYLPQENEAIN
                     DKTAYADSRMIALADTEKVLASDLMIALLDGLTIDAGVASEIGVAYAKGIPVIGLYTD
                     TRQQGGTHPKKIAALQETAENQFHYLNLYTIGLIKLNGKVVSSEIELLSEVKRFLDGG
                     TFSD"
     gene            complement(4062..5282)
                     /locus_tag="SCJ86_002573"
     CDS             complement(4062..5282)
                     /locus_tag="SCJ86_002573"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016627801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEG1170080.1"
                     /translation="MDQKDFRENLELKPVTQEHIDQFNELLSYVFQVTEADIEESGFE
                     NKRAFIRSKKPILEVSKVFGWFHENKLISQIAIYPCEVNIHGARYKMGGVTGVGTYPE
                     YANHGLMQDLIIVALDNMRKNKQWISYLYPYSIPYYRRKGWEIMSDKLSFKIRDTQLP
                     KQIPVSGMVERLPVDHPDVFSVYDKFAQQNHGALFRSDFHWEEYWRFENEDERTAAVY
                     YDSNHEPRGVLFYWVAEEIFHVKEMFYLDQEARNGLWNFISAHFSMIYWVHGDIYKNE
                     PLAFLIEDSQIKEQIEPYFMARIVDVKEFLQRFPFVGTADAFHFIIEDPVAPWNNGVF
                     ALTWDEQGQVRVLNEPIGKSVRLDIQTLTCLMMNYRRASYLARIERLETDEETLKSLE
                     RIIPNMEAYFSDYF"
     gene            5429..7264
                     /gene="lepA"
                     /locus_tag="SCJ86_002574"
     CDS             5429..7264
                     /gene="lepA"
                     /locus_tag="SCJ86_002574"
                     /EC_number="3.6.5.n1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723396.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor 4"
                     /protein_id="HEG1170081.1"
                     /translation="MDIKKMKERQEQIRNFSIIAHIDHGKSTLADRILEMTHTVTSRE
                     MQDQLLDSMDLERERGITIKLNAVELNYTAKNGETYIFHLIDTPGHVDFTYEVSRSLA
                     ACEGAVLVVDAAQGIEAQTLANVYLALDNDLEILPVINKIDLPAADPERVRQEIEDVI
                     GIDASEAVLASAKSGIGIEDILEQIVEYVPAPSGDLDAPLKALIFDSIYDSYRGVVLN
                     VRITDGMVKPGDKIKLMSNGKTFDVTEVGVFSPKAVARDFLMVGDVGYITASIKTVQD
                     TRVGDTVTLADNPAAEALPGYRKMNPMVYCGLYPIDTSRYNDLREALEKLQLNDAALQ
                     FEPETSQALGFGFRCGFLGLLHMDVVQERLEREFNLELITTAPSVIYHVNKTDGTTAT
                     VDNPADFPEPVTIQDVEEPFVKAQIMVPNDFVGAVMELSQRKRGEFITMDYLDDYRVN
                     VVYNIPLSEIVFDFFDKLKSSTKGYASLDYEMSGYQKSKLVKMDILLNGEKVDALSFI
                     VHRDFAYERGKAIVEKLKKLIPRQQFEVPIQAAIGQKIVARSDIKALRKNVLAKCYGG
                     DVSRKRKLLEKQKEGKKRMKQIGSVEVPQEAFMAVLKMDEDEPKK"
BASE COUNT         2420 a         1402 c         1505 g         2010 t
ORIGIN      
        1 tcgagaaaaa aagtcaaaaa actatgtgta tattttgacc aaaattgacc aaagcgatat
       61 aatagttttt gtaaagatca aagaaataag tgatcttacc tttaattagg agggtatacc
      121 tgtatgaata ttgagaaaat gactacgacg cttcaagaag cgattgctga agctcaacag
      181 attgcggtca ctcgaaaaca tcaagatatc gacattgccc atgtatggaa aatcttttta
      241 caatctaatc attttggtcg aaatttctat acggatgccg gattggatgt cgaatctttt
      301 gaacatgaaa ttgatcgtct actggatgag tatccagttg tttctggtgg aaacgtccaa
      361 tatggacaaa acctcagcca gaatttcttt cgattattga atgaagcaga ccaaatacgt
      421 gaaagcttcg gcgacgagtt tctttcaacg gaagtcgtta ttttagcgtt gatgaaatta
      481 aaaaattatc cgttaactgt ttatttgaat aaaaacggat tatctgaaaa agaattgcgt
      541 aaaaacatcg aagagatgag aggaggagaa agagtgacct cacaaaacca agaagaacaa
      601 tacaaagcat tagaaaaata cggcgttgat ttagtacagc aagtcaaaag cggaaaaatg
      661 gatccaatca tcggtcgtga tgaagaaatc cgtgatgtca tccgtattct ttcacgaaaa
      721 acaaaaaata atcctgttct gataggagaa cccggtgtag ggaaaacggc aatcgttgaa
      781 gggcttgctc aacgtatcgt acgcaaagat gtgcctgaaa atttaaaaga taaaacgatt
      841 ttttcattag atatgggtgc attgatcgct ggagcaaaat tccgtggtga atttgaagaa
      901 agactaaaag cagtcttgaa agaagtcaaa aaaagtgatg gacgtatcct cttgttcatc
      961 gatgaaatcc acaatatcgt aggggcagga aaaacagaag gaagtatgga tgccggaaat
     1021 ctgttgaaac caatgcttgc tcgcggtgaa ttacatttga tcggtgccac aactttagat
     1081 gagtaccgtc aatatatgga aaaagacaaa gcgttggaac gtcgctttca aaaggtatta
     1141 gtcaaagaac caacagtgga agatacgatc agtattctgc gtggattaaa agaacgtttt
     1201 gagatccacc atggtgttaa catccatgac aacgcattag tagcagcagc tactttatct
     1261 gaccgttata ttactgatcg gttcttaccg gacaaagcaa tcgatttgat cgatgaagcc
     1321 agtgcaacga ttcgggtaga aatgaactct atgccaacgg aattagatca agttactaga
     1381 cgtttgatgc agttggaaat cgaagaagct gcactgaaaa aagaatcaga tgatgcaagt
     1441 aaaaaacgct tgaaaaattt gcaagaagaa ttagctgaac ttcgtgaaga agcaaatgcg
     1501 atgaagatgc agtgggaaac agaaaaagaa gaagtcaatt ctgtttcagc aaaacgtgct
     1561 gaaatcgata aagcaaaaca cgaattagaa gatgcagaaa ataactatga tttagaacgc
     1621 gctgctgtat tgcgtcatgg tacgatacct caattagaaa aagaattgaa ggaattagaa
     1681 gctaaagcaa aagacagcga gattaagatg gttcaagagt ccgttaccga aaatgagatt
     1741 gctcaagtcg ttggcagatt aacgggtatc cccgtaacga aacttgtaga aggtgaacga
     1801 gaaaaactaa ttaaattgaa tgaaacactt cataaacgag tgattggtca agatgaagca
     1861 gttgatgcgg tcagtgacgc ggtaattcgt tcacgggctg gattgcaaga cccaaatcgt
     1921 cctctaggtt ctttcctttt cctaggacca actggtgtcg gaaaaaccga attagcgaaa
     1981 gcgttagctg agaacttgtt tgactcagaa gatcatatgg tacggatcga tatgagtgaa
     2041 tatatggaaa aacatgctgt gtctcgtcta gtcggagctc ctccaggcta tgtaggttac
     2101 gaagaaggtg gtcaattaac ggaagctgtc agacgaaatc cttatacaat cgtcttgttg
     2161 gatgaaatcg aaaaagcgca tcccgatgtc ttcaatatct tgttacaagt cctagatgac
     2221 ggacgcttga ctgattcaaa agggcgagta gtagatttca aaaatacggt tctgatcatg
     2281 accagcaata tcggttcaca gttactgctg gaaggcgtga ctgctgatgg aacgattcca
     2341 gaagcagtgg ctgaacaagt caacacgcta ttacgtggaa acttcaaacc agaattcttg
     2401 aaccgaatcg acgacacgat tttatttaca ccattgagcc tagacaacgt gaaaggaatc
     2461 gttgacaaaa tggtggcgca attggcacaa cgtctggaac atcaagaaat cctgctgaca
     2521 atcagtgatg aagccaaaac gtggattgct gaaaatgctt atgaaccagc atatggcgca
     2581 agaccattga aacgatttat cactaaggaa gtagaaacac cactagctaa agaaatcgtt
     2641 gcaggacatg ttatgcctaa atcaaaagta acgattactt tacttgatgg acaacttcat
     2701 ttcaaaacag aagagctaga agaaatcgtc taatcttcca ttgagactat aaaagaaaaa
     2761 aggggtctga cacaactagt gtcagacctc cttttttcaa ataaacagtg ttccagaagt
     2821 agtttcttcg aaaaataagc cgaaatttct tgaagctgcc cgcttctgtc acaacctctt
     2881 ttattaggta tttttcactt tttctaattt ataactttca aagtaacgct catctttttt
     2941 actttgagac catgcaaaca atcccattaa ggcagcacct atcgtatttg tcagcaagtc
     3001 gcccatcgta tccatcaatg ctgctcgtcc gacaaacagt gtgccatcag aagcagcaaa
     3061 acgttgaaga ttcatgccta agaactggtc gcataagaat tcccagaact cccaaaacac
     3121 gccgcaaagt ccagcaaacg caaagccgaa taagaggaac aaccaaggac tggtttttga
     3181 tatctcagca tctttcatca agtaaccaat caatccatag ccaagagctg tcaggaccat
     3241 tggactcacc gcatgtaaga ttttgtccca aaaagagatg atactgatca aatgcatccc
     3301 tgtgcctaaa aagacagaga tgaacaagaa aaaccagtaa aacaaaacga ttgctgctgg
     3361 tattttaagt ttgaaaactt tcaaaatgac ttgtggcatg aaaatcaatg caatccctaa
     3421 taaagattcc acgataaatg tgattccttg agaagtcgaa tatttttgtg tgataaattc
     3481 ataaaaatta tagcagacaa cgaatacacc aaaagcaagt aaagctattt gcaccttttt
     3541 gatttcttta atcactgaaa gtccctccat ctaaaaatct ttttacttct gaaagcaact
     3601 caatttctga agaaaccact tttccgttca atttgatcaa acctattgta tataagttca
     3661 ggtagtgaaa ttgattttca gctgtttctt gtagtgcagc gatttttttc gggtgggttc
     3721 cgccttgctg tcgagtatca gtatataagc cgatcactgg gatgcctttc gcataagcca
     3781 caccgatttc cgatgctacc ccggcatcga tcgtcagccc atctagtaaa gcaatcatta
     3841 aatcactagc caacactttt tctgtgtcag caagagcaat cattcgactg tctgcataag
     3901 cagttttgtc attgattgct tcattttctt gaggaagata gatcgaaagc tcagaagaga
     3961 gctctctgat ttctttaact aactgttggt tatataacaa atcactttta gcaaacattg
     4021 gtgcggcaaa ataaattttc atctaaacat catccttttt atcaaaaata atcgctaaag
     4081 taagcttcca tgtttggaat gatacgctct agtgacttca gtgtttcttc atctgtttct
     4141 aaccgttcga ttcttgccaa ataagaagca cggcgataat tcatcatcaa gcaagtcaat
     4201 gtttggatat ccagtcgaac ggattttcct ataggttcat ttaagacacg gacttgtccc
     4261 tgttcatccc atgttaaagc gaagacacca ttattccaag gagcgacagg atcttcaatg
     4321 atgaaatgaa aagcatcagc tgttccaaca aacggaaacc gttgaaggaa ttcttttaca
     4381 tcaacgatcc gagccataaa atatggttcg atctgctctt tgatctgact atcttcaatc
     4441 aaaaatgcta aaggctcatt tttataaata tctccatgta cccagtagat catggaaaaa
     4501 tgtgcggaaa taaagttcca taagccattt cgagcttctt gatcaagata gaacatttct
     4561 tttacgtgaa atatttcttc agctacccag tagaacagaa cacctcttgg ttcatggttg
     4621 ctatcgtagt agacagcggc tgtgcgctca tcttcatttt cgaaacgcca atactcttcc
     4681 caatgaaaat cactgcggaa taaagcacca tggttttgtt gggcaaattt gtcataaacg
     4741 gaaaatacgt ctggatgatc aactggtaat cgctcaacca taccacttac tgggatttgt
     4801 ttaggtagct gtgtatctct gattttaaag gaaagtttat cagacatgat ttcccatcct
     4861 ttacgccgat agtaggggat actgtaagga tacaaataag agatccattg cttattcttg
     4921 cgcatattat ccaatgcaac gataattaga tcttgcatca atccatggtt agcatattct
     4981 ggataagttc caactccagt cacaccaccc attttataac gtgcaccgtg aatattgact
     5041 tcacaaggat agatagcgat ttgagagatc agtttatttt cgtggaacca tccgaacact
     5101 ttcgatactt ccaaaatcgg ttttttggaa cggataaaag ctcgtttatt ctcaaacccg
     5161 ctttcctcga tgtctgcttc agtcacttgg aacacatagg aaagcaattc attgaattga
     5221 tcgatatgtt cttgagtgac cggttttaat tcaagatttt ctctaaaatc tttttgatcc
     5281 attttttcat ctccatttat tagcttgata acagtatagc aaaattctac aaaaaaaact
     5341 tctatagctg actaattaaa aactctttaa attactgctt ttcgttggta taatggaaag
     5401 tgctgattat atgaaaagag gagacgctat ggatataaag aaaatgaaag aacgccaaga
     5461 gcagattcgt aatttttcga tcatcgctca tatcgaccat gggaaatcta cattggccga
     5521 tcgtatttta gaaatgacgc acactgtgac gtctcgcgaa atgcaagatc agctgttaga
     5581 ctcgatggat ttggaacgtg aacgtggaat cacgattaaa ttaaatgctg tagaactcaa
     5641 ttacacagcg aaaaatggag aaacatatat ctttcacttg attgacaccc cagggcacgt
     5701 cgacttcacc tatgaagtct ctcgaagcct tgctgcctgt gagggtgccg ttttagtggt
     5761 cgatgcagca caaggaatcg aagcacaaac attggccaat gtttatttag cgttagacaa
     5821 tgatttggag atcctgccag tcatcaataa aatcgaccta cccgcagcag atcccgaacg
     5881 tgtccgtcaa gaaatcgaag acgtaatcgg aatcgatgct agtgaagctg tattagccag
     5941 tgccaaatca gggattggga ttgaagatat tctcgagcag atcgtggaat atgttcctgc
     6001 accaagtgga gacttagatg caccgctaaa agctttgatc tttgactcta tttatgatag
     6061 ttaccgcggt gttgttctaa atgtccggat cacggacggt atggtcaaac ccggagacaa
     6121 aatcaaactg atgagtaatg gcaaaacatt tgatgttaca gaggtcgggg tcttctcgcc
     6181 aaaagcagtt gctagagact ttttgatggt cggtgatgtc ggttatatca cagcaagtat
     6241 caaaactgtc caagatacac gagtagggga tacagtaacg ttagcagaca atcctgccgc
     6301 tgaagcttta ccaggttacc gaaaaatgaa tccgatggtt tattgtggtc tttatccaat
     6361 tgatacttct cgttacaacg atttgcggga agctttagaa aaactgcaat tgaatgatgc
     6421 tgctttacaa ttcgaaccag aaacctctca agcattaggt tttgggttcc gctgtggatt
     6481 cctcggactt cttcatatgg atgtcgtcca agaacgcttg gaacgggaat tcaatctaga
     6541 attgatcacg actgcaccat ccgttattta ccatgttaat aaaacggatg gtacgactgc
     6601 tacagtagat aacccggcag attttcctga acctgtaacg attcaggatg tagaagaacc
     6661 attcgtcaaa gcacagatca tggtcccaaa tgattttgtc ggtgcagtaa tggaactttc
     6721 tcaacgaaaa cggggagaat tcattacgat ggattattta gatgattacc gagtaaatgt
     6781 cgtctataat attccgcttt ctgaaattgt ctttgacttt ttcgacaaat tgaaatcaag
     6841 cacgaaaggc tatgcctcat tggattatga aatgtctggt tatcaaaaaa gcaagttagt
     6901 gaagatggac atcttgctga atggagaaaa agtcgatgca ttaagcttta tcgttcaccg
     6961 agactttgcc tatgaacgtg gaaaagcaat cgttgaaaaa ctgaaaaaac taatcccacg
     7021 ccaacaattt gaggtgccaa tccaagcagc tattggacaa aaaatcgttg cccgttcaga
     7081 catcaaagcc ttgcgtaaaa acgtcttggc taaatgttat ggtggagatg tttctcgtaa
     7141 acgtaaactc ttagagaaac aaaaagaagg gaagaagcgg atgaaacaaa tcggctccgt
     7201 cgaagttccg caagaagcct tcatggccgt tctgaaaatg gacgaggacg aaccgaagaa
     7261 atagcaaggg tttgtttgaa aacagtgtaa aaaataaacc atgtttacat ggatttgcca
     7321 ccggtttgcc accggtg
//
LOCUS       DARBET010000103         6936 bp    DNA     linear   BCT 07-NOV-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4027
            SAMEA114569841-rid20112183.denovo.107, whole genome shotgun
            sequence.
ACCESSION   DARBET010000103 DARBET010000000
VERSION     DARBET010000103.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA114569841
            Sequence Read Archive: ERR12203666
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 6936)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6936)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001972618.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001972618.1
            Genome Coverage       :: 132x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/07/2023 13:18:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,041
            CDSs (total)                      :: 2,975
            Genes (coding)                    :: 2,908
            CDSs (with protein)               :: 2,908
            Genes (RNA)                       :: 66
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 67
            CDSs (without protein)            :: 67
            Pseudo Genes (ambiguous residues) :: 0 of 67
            Pseudo Genes (frameshifted)       :: 26 of 67
            Pseudo Genes (incomplete)         :: 28 of 67
            Pseudo Genes (internal stop)      :: 20 of 67
            Pseudo Genes (multiple problems)  :: 6 of 67
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6936
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA114569841-rid20112183.denovo.107"
                     /isolate="VRE4027"
                     /db_xref="taxon:1352"
                     /country="Denmark"
     gene            complement(1..1151)
                     /locus_tag="SCJ86_002575"
                     /pseudo
     CDS             complement(1..1151)
                     /locus_tag="SCJ86_002575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611166.1"
                     /note="frameshifted; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS3-like element ISEfa10 family transposase"
     gene            1394..1849
                     /gene="tnpA"
                     /locus_tag="SCJ86_002576"
     CDS             1394..1849
                     /gene="tnpA"
                     /locus_tag="SCJ86_002576"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS200/IS605 family transposase"
                     /protein_id="HEG1170082.1"
                     /translation="MSNDDKSLAYTRWNCKYHIIFTPKYQRKVIYGQLRKDLGKILRK
                     LCEMKDVEIIEAHAMLDHIHMLVRVPPKICISSFMGYLKGRSAVLIHEQHANLKYKYG
                     NKSFWSKRYYVSTVGLNQKTNEKYIREQEADDRIRYIISKREYVEPFKK"
     gene            2114..2212
                     /locus_tag="SCJ86_002577"
                     /pseudo
     CDS             2114..2212
                     /locus_tag="SCJ86_002577"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339652.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS30 family transposase"
     gene            complement(2895..3713)
                     /locus_tag="SCJ86_002578"
     CDS             complement(2895..3713)
                     /locus_tag="SCJ86_002578"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313161.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MurR/RpiR family transcriptional regulator"
                     /protein_id="HEG1170083.1"
                     /translation="MSYIYLIKENYPFLTKSEKKVADFILNSGKTIIYSTMSDIKKNT
                     KVGDATIIRFCQKIGFSGFSDLKIEIAKEDFSQKKEHPTSVKYYDEIANSLTEALYST
                     IRLLDEEKLNEAIKMITQSKNIYIFGVGSSGNTSLDLENMFLRVGVQAKAVLDPHFQA
                     QVASLLTVNDLVIIFSLSGKTKDTYDSLKIAKKNGAKIIAITNYIHSPIGKSADLVLQ
                     TAIEEFLNGGSLAGKISQLYICDLLVHGYEQNNNIDSVELREKVLRSIIDKSIE"
     gene            3874..4563
                     /locus_tag="SCJ86_002579"
     CDS             3874..4563
                     /locus_tag="SCJ86_002579"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002358533.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmannosamine-6-phosphate 2-epimerase"
                     /protein_id="HEG1170084.1"
                     /translation="MDKKRFLDLISKGLIVSCQALPGEPFYKEEESLMPYFALAAQRA
                     GAVGIRANSVRDIIEIRKCVSLPMIGIIKRDYPPESPFITATMREVDELVETGVEVIA
                     LDCTLRNRHDGLSINSFITKIKEKYPHQLFMADISTFEEGKNAYKAGIDFVGTTLSGY
                     TEESKKQDGPDIDLIKKLVEEGICVIAEGRIHTPEQAKEIKKIGVAGIVVGGAITRPQ
                     EIASRFVKALS"
     gene            4577..6079
                     /locus_tag="SCJ86_002580"
     CDS             4577..6079
                     /locus_tag="SCJ86_002580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002367389.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HEG1170085.1"
                     /translation="MFKKFSQLGRAFMLPIAILPVAGLLLGLGGALTNESAINAYPFL
                     DQPWLHTILSIMSYAGNAVFANLALIFAIGIAVGLANGDKGTAGLAGGVAYLVYTATI
                     SGFLELFSAKDATLDTGVVGSIVVGGTVAFLHNRYRKIELPQFLGFFGGSRFIPIVSS
                     LAAIIIGSFFYLIWPPIQNGLVVAGEQIAQMGSLGTFLYGFLLRLTGAVGLHHTIYPL
                     FWYTSLGGTETVAGSTIAGAQNIFFAQLADPNHTGLFTYGTRFFAGRFATMMFGLPAA
                     CYAMYRAIPKKNRKKNGGLYFSGALTSFLTGITEPVEYMFLFVAPWLYVIHAFLDGLS
                     FYFADILNIRVGNSFSGGLIDFLLFGVLQGNDKTNWLKLIPFGIAWAFIYFFVFTFFI
                     KKFKVAIPGMEEESEVHSEKVINKNTSLHEESLIIIEALGGKENIESVEACATRLRVA
                     VTNGEIIQKQTIKDTGATAVFEVKGGIQAVFGGKADLLSQEINQILGKDN"
     gene            6092..6568
                     /locus_tag="SCJ86_002581"
     CDS             6092..6568
                     /locus_tag="SCJ86_002581"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300328.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucose transporter subunit IIA"
                     /protein_id="HEG1170086.1"
                     /translation="MFEIFKKKKQETFYAPCSGKIISITEVNDPVFSQKLLGDGFAVI
                     PDGSKIYAPLAGRVTNIFPTKHAINIKSNSGIDYLIHIGIDTVELQGEPFSICVQEND
                     EVTPETSLVDVDFDKIIKSGKDTSVIVVFPEKNQFNDLIIDNGRIAHGNSCGKITF"
     gene            complement(6665..6775)
                     /locus_tag="SCJ86_002582"
                     /pseudo
     CDS             complement(6665..6775)
                     /locus_tag="SCJ86_002582"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016252604.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
BASE COUNT         2252 a         1092 c         1265 g         2327 t
ORIGIN      
        1 ccaaccttga tctgagtgaa acgtacgacg atagggacaa tcgttagtgg tctggatagc
       61 ttcctccaaa ccttccatta ccgctaatgc attgggcttt tcagagatac gataggataa
      121 aatctcacta ttgaacatat ccataaaagg atctagatac aatttctttt gacggataat
      181 gcctttggca tccacttcat ggtatttaaa ttcagttgta tccgtcgtga ttttctgatg
      241 gcagatactg gtgtagaaac gacgatggat ccgatttttg gctactgctc cgacttgtcc
      301 tcgataagaa ttgtagcgcc gtgatttacg tgtgtatgtc gttactttta ttcctagctt
      361 cttgatcagg cgctgaactt tctttttatt gatagagaaa ccgcggtttc ttagttcatt
      421 ggtcatccgt aaacagccat aatccttgtg cttctcccga actttaagca tttccgcttc
      481 aatctcttga tctggatttt tacggtccaa tcgtttttgc cagtacataa agattgcctt
      541 agggaacttt aaagtttcta gaagctcaac taatttaaac ggtcctcgga ggcttgcgat
      601 gattcgtgcg attgcttcat tcgtcgttgt tgggcttcct gttttcgcag cttcctcaat
      661 tcttttaaaa aggcattttc aatttgaagg gaacggactc gtttctccaa ctcctcgatg
      721 cgttcacgtt ctttagacgt tgctttagtt gtagattttt tcttattttt gggttcattt
      781 ttcttagaca agataggtgg acatcctttc gtttttgaga gtccatcgat cccaccctca
      841 cggaattgac gcatccaact agcgatcaaa ttagggttgt tcatgttaag tgtgttggca
      901 acttcacgat aggacagctc acttgttaga tataactcta tcgcatccag cttaaattga
      961 acagaatatt ttttcttttg tcgctttctt actagaccct cttcaccaaa ttcttgatag
     1021 gcattgatcc agcgcttcac ttgtgatgga gccttaatgg aatatttctt tgccagataa
     1081 tcacttccgc ctttaccatc caagtaatca tgaacgatct ttaatttaag ttcaaaagta
     1141 tacttagcca taaaaaatac cgacctccaa aagttagatt ttttggtcta acttttgggg
     1201 gtcggtacaa aacttaaaag ttttagaccc ttttattttc ttaatgactt cagtcagttt
     1261 tgtgcgtaaa gcgtgcgaaa caagaaaacc gcgcgtttct acgcgtggaa gaagtatctt
     1321 tagcgataaa aactcctttc gatatactag tgagtggcta ccaaccgctc atcagatcga
     1381 aaggagtttt ttcatgtcaa atgacgataa aagtttagca tatactcgtt ggaattgtaa
     1441 gtatcatatt atttttacac caaaatatca acgaaaagta atctatgggc aattacgaaa
     1501 agatcttgga aaaatactta ggaagttgtg cgaaatgaaa gatgtggaaa tcatagaagc
     1561 acatgcaatg ctagatcata ttcacatgct tgttcgagta ccaccaaaaa tatgtatatc
     1621 cagttttatg gggtatctga aaggtcgtag tgcggtacta atccacgagc agcacgcaaa
     1681 tctcaaatat aaatacggaa acaaatcctt ttggtcgaag agatactatg tgagtacagt
     1741 cggattgaat caaaagacaa atgaaaaata catccgtgaa caagaagctg atgatcgcat
     1801 tcgatatatt ataagtaaaa gagagtacgt agaaccattt aaaaaataaa gagaagtaag
     1861 tgagtgaaga tgagcagttg gcggtctttt aaaaatcccc ttttaagggg cagcgagtgt
     1921 cgaccccttc tagggggatt aaaaatacac ccgtcctcac gggtgtatgg gttactgata
     1981 gacttgattt taactggtgt cagaaactta tgtccagaaa gggtgatgaa attttggcaa
     2041 tttacggcta ttcaagaatt agcacaagca accaagatta caaaacacaa attcaaacat
     2101 tagaaaatgc tggcgcagaa ataatcttcc tagaaaatca ctggattatc gaacaccttt
     2161 ggaagtattt ttgagttacg taagtattga tgatctgtct aacttaattt gacaattaag
     2221 agtatattta cttcagatgg taaggcaaca aaataaaata caataggcag atgaactaat
     2281 aaataaaatc tgtattaaat acagataaat taatataaaa atcaaaagga tcataaactt
     2341 ttaacaagtg atgaaatggg tggaaattga ttcttataga cagtttgtct cataacattg
     2401 aagtattttg caattttagc attgaaacac ttttttaatc ttcaacgttg ttcttataaa
     2461 caagatgatt gggaatcttt tgaattaaca ctgtatagct tagaacaccc tttatagaaa
     2521 acattcgctt agcaatctta ataattcctt gtgcccgatg tcgtttagat tcagtaggtt
     2581 cctgctcatc gatcatggaa aaaatttgaa ggatcagatc cttaataaat ttatttaaga
     2641 gaggatgatc cattgcttca tccatactcg ataaactagt gataatgagc tacacgtttt
     2701 tctttcaagt tgatcaatag cttccacgat aaaaatatct ttagataaaa tcaagaaccg
     2761 taggaactac agggatattt ggtcaataag agccaccgct attagtaaaa aataagctat
     2821 caagtatgct ctcatcccaa gaattgccta cttcaatcgt taaaaaccgt taggtttagc
     2881 taagtggggg tagttcactc aatactttta tcaataatag agcgcaaaac tttttctctt
     2941 aattcaactg aatcaatatt attgttttgt tcataaccat gaactaatag atcacaaata
     3001 taaagttgtg atatttttcc agctaaagat ccaccattaa gaaattcttc aatggctgtt
     3061 tgtaaaacta agtctgcaga ctttcctata ggtgaatgaa tatagttagt tatagcaata
     3121 atttttgcgc catttttttt agctattttc aatgaatcat aagtatcttt cgttttccca
     3181 gataaagaaa aaatgattac taaatcatta actgttaata aagaagcgac ttgggcttga
     3241 aaatgagggt ctagtacagc ttttgcttga actcctacgc gaagaaacat attttctaaa
     3301 tcaagactag tatttccact agatcccact ccaaatatat atatgttttt tgattgagtg
     3361 atcattttaa tggcttcatt caatttttct tcgtctaaaa gcctaatagt ggaatagaga
     3421 gcttctgtta aactattagc aatttcatca tagtacttta cactagtcgg atgttctttt
     3481 ttttgactaa agtcttcctt tgcaatttca atttttaaat cagaaaaacc agaaaaacca
     3541 attttttgac aaaagcgaat aatagtagca tcgccaactt ttgtgttttt cttaatatca
     3601 ctcatcgtac tataaataat agttttccca gaatttaaaa taaaatcagc gacttttttt
     3661 tcagacttcg ttaaaaatgg ataattttct ttaattaaat agatataact catacaaact
     3721 cctctacaat ttcaactttt ctccattata ctccaaaata ataaaaaaaa ctcctaaata
     3781 ctccattttt atctttactt ttatgaaagc ggatgctata ttgtttttgg agttgttatc
     3841 caaaaataat aaaaaattgg agtgtttagg ataatggata aaaagaggtt tttagatctt
     3901 atttcaaaag gtttgattgt ttcctgtcaa gcattacctg gtgaaccatt ttacaaagag
     3961 gaagaaagtt taatgcctta ttttgctttg gctgctcaaa gggctggagc agttggtata
     4021 cgtgcaaatt ctgttcgaga tattattgaa attagaaagt gtgtatcatt accaatgatt
     4081 ggaattatta aaagagatta tccaccagaa tctccattca ttactgccac tatgagagaa
     4141 gtagatgaat tagtagaaac cggtgttgag gtaatcgctc ttgattgcac tcttagaaat
     4201 cgacatgatg gactatctat aaattctttt attactaaaa taaaagaaaa atatcctcat
     4261 cagttattta tggcagatat ttctacattt gaagaaggca aaaatgcata taaagcagga
     4321 atagattttg taggaactac tttaagtgga tatactgagg aaagtaaaaa acaagatggg
     4381 ccagatatag atttgattaa aaaattggta gaagaaggga tttgtgtaat tgctgaggga
     4441 aggattcata ctccagaaca agcaaaagag ataaaaaaaa taggagttgc aggtattgtt
     4501 gtgggtggtg ctattactcg accacaggaa attgcatcaa gatttgttaa agcattaagt
     4561 tagggggaga aataaaatgt ttaaaaaatt ttcacaatta ggacgcgctt tcatgttacc
     4621 aattgctata ttaccagtag ccggattact attgggactg ggaggagcgt taacaaatga
     4681 gagtgctata aatgcttatc catttttaga tcaaccgtgg ttgcatacaa ttttaagtat
     4741 tatgagttat gctggaaatg cagtttttgc aaacctagca ctaatttttg caattggtat
     4801 tgctgtagga cttgcaaatg gagataaagg aacagctggt ttagctggtg gagtagctta
     4861 tctagtatat actgcaacta ttagtgggtt tcttgaactt ttttcagcaa aagatgcaac
     4921 actagataca ggtgtagtgg gatcaattgt tgttggtggt actgttgcat ttttacacaa
     4981 tcgttataga aaaattgaat taccacaatt cttaggtttt tttggaggat caagatttat
     5041 tccgattgtt tctagtttag ctgcaatcat tattggttct ttcttttatt taatttggcc
     5101 accaattcaa aatggtttag tagttgctgg tgaacaaatt gcccaaatgg gtagtttagg
     5161 aactttttta tatggttttt tattgcgttt aacaggtgct gttggactac accacaccat
     5221 ttaccctttg ttctggtata catctttagg tggtaccgaa actgtagctg gatcaacaat
     5281 tgctggagcc caaaatattt tctttgccca actagctgat ccaaaccaca ctggtctgtt
     5341 tacttatggt actagatttt ttgcaggaag atttgcaaca atgatgtttg gattaccagc
     5401 agcatgttat gcaatgtatc gtgcaattcc taagaaaaat cgtaaaaaga atggtgggct
     5461 ttattttagc ggggccctta catcattttt aactggaatt actgaaccag ttgaatatat
     5521 gtttttattt gttgctcctt ggctttatgt gattcatgca ttcttagatg gattatcttt
     5581 ttattttgct gatatcttaa atattcgtgt agggaattcc ttttcaggtg gtttgatcga
     5641 ctttttatta ttcggtgtac ttcaagggaa tgacaaaact aattggttaa aattaattcc
     5701 ttttggtatt gcatgggcat ttatatattt ctttgttttt acattcttta tcaaaaaatt
     5761 taaggtagct attcctggaa tggaagaaga atcagaagtt cattctgaaa aagtaataaa
     5821 caaaaataca tcattacatg aagagtctct tatcattatt gaagctcttg gtggaaaaga
     5881 aaatattgag tctgttgaag cttgtgcaac tcgtttgcgt gttgctgtta cgaatgggga
     5941 gattattcaa aaacaaacaa taaaagacac tggtgcaact gctgtcttcg aagtgaaggg
     6001 tggcatccaa gctgtttttg gagggaaagc tgacttatta agtcaagaaa tcaatcaaat
     6061 attaggaaaa gataattagg aggtttttaa tatgtttgaa attttcaaaa agaaaaaaca
     6121 agaaactttt tatgcacctt gttccggaaa aatcatttcc atcacagaag taaatgatcc
     6181 tgtcttttca caaaaattac taggtgatgg ttttgccgtc atacctgatg gttcaaaaat
     6241 ttatgctcca ttggctggtc gagtaacaaa tatatttcct actaaacatg ctattaatat
     6301 aaaaagcaac tcaggcattg attatttgat tcatattgga attgatacag ttgaattaca
     6361 aggagagcct ttttcgattt gtgttcaaga aaatgatgag gttactcctg aaacctcgtt
     6421 ggttgatgtg gattttgaca aaataataaa atctggtaaa gatacttctg ttatagttgt
     6481 ttttccggag aaaaatcagt tcaatgattt aataatagat aatggtcgaa ttgctcatgg
     6541 gaattcatgt ggaaaaatca ctttttaaaa aagtgtatat aaaaagctat aaaataagct
     6601 aaataggata tttgtcaaat gaagttggtg aacagaaaga actgttttct ttctgttcat
     6661 ttttttattt tattagtaat gattccccat gccaaatcat gatccgtaat ttaaagttat
     6721 aaaagcttcg aaatccattg gcatttcgtt ggaatacctt gatatgatta ttcaaatata
     6781 aaatatagag agaagagaga gagggaagat atttataaag ggcaaagtta tgtttactca
     6841 acttaggaaa tagaaataga tcaatttata ttctcttgtc aatcgaatat gaaccttagc
     6901 actatatacc aaatatagta aaatgctgaa agatgt
//
LOCUS       DARBET010000104         6930 bp    DNA     linear   BCT 07-NOV-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4027
            SAMEA114569841-rid20112183.denovo.108, whole genome shotgun
            sequence.
ACCESSION   DARBET010000104 DARBET010000000
VERSION     DARBET010000104.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA114569841
            Sequence Read Archive: ERR12203666
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 6930)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6930)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001972618.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001972618.1
            Genome Coverage       :: 132x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/07/2023 13:18:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,041
            CDSs (total)                      :: 2,975
            Genes (coding)                    :: 2,908
            CDSs (with protein)               :: 2,908
            Genes (RNA)                       :: 66
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 67
            CDSs (without protein)            :: 67
            Pseudo Genes (ambiguous residues) :: 0 of 67
            Pseudo Genes (frameshifted)       :: 26 of 67
            Pseudo Genes (incomplete)         :: 28 of 67
            Pseudo Genes (internal stop)      :: 20 of 67
            Pseudo Genes (multiple problems)  :: 6 of 67
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6930
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA114569841-rid20112183.denovo.108"
                     /isolate="VRE4027"
                     /db_xref="taxon:1352"
                     /country="Denmark"
     gene            complement(61..519)
                     /locus_tag="SCJ86_002583"
     CDS             complement(61..519)
                     /locus_tag="SCJ86_002583"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucose transporter subunit IIA"
                     /protein_id="HEG1170087.1"
                     /translation="MHFFKTRNILKAVASGQIIPLTAVKDEVFSSKMIGVGYAITNHD
                     GYIHAPVKGIIDNIFPTRHAITIESESGRKILIHMGLDTVELEGSPFQIRVESNQEVK
                     EGTLLAIIDLKKLEQAQKDTVIIVVSPGIFEGNLLKQNQEVKIKDSCFEF"
     gene            complement(532..1863)
                     /locus_tag="SCJ86_002584"
     CDS             complement(532..1863)
                     /locus_tag="SCJ86_002584"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002361883.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="maltose-6'-phosphate glucosidase"
                     /protein_id="HEG1170088.1"
                     /translation="MMMKKQIITIAGGGSTYTPGIVQAILNNEKRLPLSEIRLYDIDE
                     ERNMDMYLIVKFMLKRKGFSNIRIRATDDPKLAFTGCDFVFSQIRVGGLEMREKDEKI
                     PLKHGLVGQETCGLGGFAYGMRSIKGLLEIVDHIQDYAPKAWILNYTNPESIVSEAVR
                     RKYPNVKMINACDMTISIEETIAVNYGYDRKNWIVTYYGLNHFGWYTSIYDKELQREI
                     MPEIIEKLITKEMQVADFNIGDKTWQKTFQMMSVITKNFPSNIPNNYLEYYLYPDMVV
                     EHTDKEYTRANMVMDGREKNTKEMADKIRRGIVEEVLNFNFGEHGQYIVDIAISLLND
                     DRRRFMLIVPNQGAIPNLRSDAVVEIPAYVGATGVEPITLRKPISDFHKGLMEAQVAV
                     EKLLVDAYFEGSYQKALQAFTLNQTVPNARVAKKILDEMIKVNKKYWPALK"
     gene            complement(1874..3316)
                     /locus_tag="SCJ86_002585"
     CDS             complement(1874..3316)
                     /locus_tag="SCJ86_002585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321325.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HEG1170089.1"
                     /translation="MRIFNRGGIKMNVKSNVQKLGRAMLLPVAAMPLAGLIMRLSADD
                     MLNIPVIGAAGNAVFGNLDILFAIGVAIGFVKNKDKGIPALTGFLAIATLKKGLEIMD
                     PEVNMGIFGGIISGLVAAWTYNRFKEQKLPVVFSYFAGEKFPLTMVMILQTIAAVFLG
                     FIWPLIQIGIDNFAKLLVDMGALGVGIFMFLNRLLIPFGLHHVLNTYIYYDLGSYTSP
                     SKEVFRGEMTRFINGDPQAGLFLSVFFVVMMFGIPAICLAIYKAAYKENKEMVKGIMG
                     SGAGTSFIANITEPVEFSFMFLSPMLYVIHALFAGLAGIVCYLFNIRIGFTFGACIVD
                     YLINFRIATNAILILPIGIFFFALYYVTFYYLINKRNIQTLGREAKAEFGNEVTLEET
                     ELGLASKNYEYMATKMLQAFGGKANILDVYSCNTRLRVEVVDPTMVEEQRIKQLGISG
                     IIKPTEKNYQIIIGLEVTYVMAEFNKLLEE"
     gene            complement(3303..4067)
                     /locus_tag="SCJ86_002586"
     CDS             complement(3303..4067)
                     /locus_tag="SCJ86_002586"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318971.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate deacetylase"
                     /protein_id="HEG1170090.1"
                     /translation="MGKVIFNSDDFGYSHGVNYGIMDAYQRGILTSTTLMANMPGFEH
                     AVKLRKEMPRLGVGVHLTLTCGKPLLKTVDTLIESGEFRSLSYYQHPFNIDKDQLYQE
                     WNAQIQKIYHAGIIPTHLDSHHHTHTLDENQEVVVTLAKKYDLPVRSNFERKDEVRHV
                     DYFEPCFDVVGETDEKKRQINTSVEEYLASLLMILREDKTIEVMCHAAYLDQELLNGS
                     SFVYPRTHQVEFLIHSAFAQKVKNDPLIELVTYEDI"
     gene            complement(4171..5046)
                     /locus_tag="SCJ86_002587"
     CDS             complement(4171..5046)
                     /locus_tag="SCJ86_002587"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317647.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PRD domain-containing protein"
                     /protein_id="HEG1170091.1"
                     /translation="MKESASCFVLSYRGGELTVYVMKKLNHNAVLIDDDGIEKIAMGK
                     GIGFSAKVSKPFDVEDADKLFVLDSKEKIKRFSEIVSQIPIEFMDFAEEVIELITARI
                     SSPLDSNIYITLTDHIYFAIQRKREDRDVTAIMLPEMRFLYPNEYDIAVEVVERINHR
                     YQTELGSNEVGFITMHIINAELGEPNSLKSLKIVEMTTTILSHLEKSYSNVYDKESLT
                     YHRLMVHIKFLVKRLIYQEETPVENIGFFTETFKHSKAYCTALKIKDIIEQQFQLAIQ
                     ENEIIYLAIHLARIY"
     gene            complement(5301..5385)
                     /locus_tag="SCJ86_002588"
     tRNA            complement(5301..5385)
                     /locus_tag="SCJ86_002588"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(5349..5351),aa:Leu,seq:caa)
     gene            complement(5403..5474)
                     /locus_tag="SCJ86_002589"
     tRNA            complement(5403..5474)
                     /locus_tag="SCJ86_002589"
                     /product="tRNA-Gln"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(5440..5442),aa:Gln,seq:ttg)
     gene            complement(5484..5556)
                     /locus_tag="SCJ86_002590"
     tRNA            complement(5484..5556)
                     /locus_tag="SCJ86_002590"
                     /product="tRNA-His"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(5521..5523),aa:His,seq:gtg)
     gene            complement(5618..5702)
                     /locus_tag="SCJ86_002591"
     tRNA            complement(5618..5702)
                     /locus_tag="SCJ86_002591"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(5666..5668),aa:Leu,seq:caa)
     gene            complement(5723..5793)
                     /locus_tag="SCJ86_002592"
     tRNA            complement(5723..5793)
                     /locus_tag="SCJ86_002592"
                     /product="tRNA-Cys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(5759..5761),aa:Cys,seq:gca)
     gene            complement(5839..5910)
                     /locus_tag="SCJ86_002593"
     tRNA            complement(5839..5910)
                     /locus_tag="SCJ86_002593"
                     /product="tRNA-Gln"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(5876..5878),aa:Gln,seq:ttg)
     gene            complement(5922..5994)
                     /locus_tag="SCJ86_002594"
     tRNA            complement(5922..5994)
                     /locus_tag="SCJ86_002594"
                     /product="tRNA-His"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(5959..5961),aa:His,seq:gtg)
     gene            complement(5997..6070)
                     /locus_tag="SCJ86_002595"
     tRNA            complement(5997..6070)
                     /locus_tag="SCJ86_002595"
                     /product="tRNA-Trp"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(6036..6038),aa:Trp,seq:cca)
     gene            complement(6077..6157)
                     /locus_tag="SCJ86_002596"
     tRNA            complement(6077..6157)
                     /locus_tag="SCJ86_002596"
                     /product="tRNA-Tyr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(6121..6123),aa:Tyr,seq:gta)
     gene            complement(6159..6234)
                     /locus_tag="SCJ86_002597"
     tRNA            complement(6159..6234)
                     /locus_tag="SCJ86_002597"
                     /product="tRNA-Phe"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(6199..6201),aa:Phe,seq:gaa)
     gene            complement(6270..6342)
                     /locus_tag="SCJ86_002598"
     tRNA            complement(6270..6342)
                     /locus_tag="SCJ86_002598"
                     /product="tRNA-Asp"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(6307..6309),aa:Asp,seq:gtc)
     gene            complement(6352..6424)
                     /locus_tag="SCJ86_002599"
     tRNA            complement(6352..6424)
                     /locus_tag="SCJ86_002599"
                     /product="tRNA-Val"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(6389..6391),aa:Val,seq:tac)
     gene            complement(6440..6511)
                     /locus_tag="SCJ86_002600"
     tRNA            complement(6440..6511)
                     /locus_tag="SCJ86_002600"
                     /product="tRNA-Glu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(6476..6478),aa:Glu,seq:ttc)
     gene            complement(6526..6615)
                     /locus_tag="SCJ86_002601"
     tRNA            complement(6526..6615)
                     /locus_tag="SCJ86_002601"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(6579..6581),aa:Ser,seq:gga)
     gene            complement(6655..6728)
                     /locus_tag="SCJ86_002602"
     tRNA            complement(6655..6728)
                     /locus_tag="SCJ86_002602"
                     /product="tRNA-Asn"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(6692..6694),aa:Asn,seq:gtt)
     gene            complement(6738..6853)
                     /gene="rrf"
                     /locus_tag="SCJ86_002603"
     rRNA            complement(6738..6853)
                     /gene="rrf"
                     /locus_tag="SCJ86_002603"
                     /product="5S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00001"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00001"
BASE COUNT         2070 a         1541 c         1140 g         2179 t
ORIGIN      
        1 caaaaaaatt ttgtaaattc acccataaat atcaccctat ttaacttttt tcatcgtaca
       61 ctaaaattca aaacaactat ctttaatctt tacttcttgg ttttgtttta ataaatttcc
      121 ttcaaaaatg cctggagaaa ctacaatgat caccgtatct ttttgtgctt gctccaactt
      181 cttcaaatct ataatagcta acagtgtccc ttcttttacc tcctggttac tttcaacccg
      241 tatctgaaac ggacttcctt cgagttcaac tgtatctaaa cccatatgga tcagtatttt
      301 tctgccagat tcactttcga tcgttatggc atgtcgggta ggaaatatat tatcgattat
      361 tcctttaact ggcgcatgga tatatccatc atgattcgtt atagcgtatc caactcctat
      421 catttttgaa gaaaagacct cgtcttttac tgctgttaat ggaataattt gaccactagc
      481 tacagctttt agaatgtttc ttgttttaaa gaaatgcact ttttatcacc tctactttaa
      541 tgctggccaa tattttttgt ttactttgat catttcatct agtatttttt ttgcgactct
      601 ggcatttggt acggtttgat tcaatgtaaa tgcttgtaaa gctttttgat aagaaccttc
      661 aaagtaagca tctactagta acttctctac tgcaacttgt gcctccatca atcctttatg
      721 aaaatcactg attggttttc ttagtgtaat tggctccaca ccagttgcac caacatatgc
      781 cggtatttca acaactgcat ctgaccgtag attcggaatc gccccttgat taggaacgat
      841 tagcataaag cgacgacgat catcattcaa caaggaaata gctatatcga cgatatattg
      901 tccatgctcc ccaaaattaa agttcaatac ttcttccaca ataccgcggc gtattttatc
      961 agccatttct ttggtatttt tctctcgtcc atccatgacc atattagcac gtgtatactc
     1021 tttgtcagta tgctcaacga ccatatcagg atataagtaa tactctaagt aattattcgg
     1081 gatattactt ggaaaatttt tcgtgatgac tgacatcatt tgaaaggttt tttgccaagt
     1141 tttatctcct atattaaaat cggcgacttg catttctttt gttattagtt tttcaataat
     1201 ctctggcata atttctcgtt gcaattcttt atcataaatc gatgtatacc agccaaagtg
     1261 gtttaaacca taatacgtaa cgatccaatt cttacgatca taaccgtaat tcactgcaat
     1321 cgtttcttca attgaaatag tcatatcaca ggcattaatc atttttacat tcggatattt
     1381 acgacgaaca gcttcagaaa cgatcgactc tggatttgta taattcaaaa tccatgcttt
     1441 gggggcatag tcttgtatat gatcgacaat ttccaataaa cctttgattg agcgcatccc
     1501 ataagcaaat cctccgagtc cacacgtttc ttgtcctact aatccatgtt taagtggaat
     1561 cttttcgtcc ttttctcgca tctccaatcc tccaacacga atttgtgaga aaacaaaatc
     1621 gcatcctgta aaagcaagct ttggatcatc cgttgcacga atgcgaatat ttgaaaaacc
     1681 ttttcttttc agcataaatt taacaatcaa gtacatatcc atatttcttt cttcatcaat
     1741 atcatataaa cgtatttcag ataatggcaa ccgtttttca ttgttcaaaa tggcttgaac
     1801 gattcctggt gtatacgtac tcccgcctcc agctatagtg ataatctgtt ttttcatcat
     1861 catcttttcc tttctattct tctaataatt tattgaattc tgccataaca taagtcactt
     1921 ctaagccgat gataatttga taattttttt cagttggttt aatgatcccg cttatcccta
     1981 attgcttgat tcgctgttcc tcgaccatgg tgggatccac tacctctacc cgtaggcgtg
     2041 tattacaact atagacatcc aggatattgg cttttcctcc aaacgcttgt agcatttttg
     2101 ttgccatata ttcataattt ttacttgcta accctaactc tgtttcttct aaggttactt
     2161 cattgccaaa ctctgcctta gcttcacgtc ctaatgtctg aatgtttctc ttattgatca
     2221 aataataaaa agtaacgtaa tacaaggcaa aaaagaaaat tccgattgga agaatcaaaa
     2281 tagcattggt agcgattcga aaattgatga gataatcaac aatacatgct ccgaatgtaa
     2341 aaccaattcg tatattgaat aagtaacaga caatccctgc taaaccggca aaaagtgcat
     2401 ggatcacgta caacatagga gaaagaaaca tgaaactaaa ttcaacaggc tcagtgatat
     2461 ttgcaataaa tgaagttcct gcaccgcttc ccatgattcc tttgaccatc tctttatttt
     2521 ctttataagc tgctttatag atagctagac atatagctgg tataccaaac atcattacta
     2581 caaaaaaaac tgataaaaac agtccagcct gaggatctcc gttgataaag cgtgtcatct
     2641 ctcctctgaa aacttccttg ctaggagatg tatagcttcc taaatcgtaa taaatatacg
     2701 tattcaacac atggtgcagt ccaaagggga tcagcaaacg attcaaaaac ataaatatcc
     2761 ccacacctaa tgctcccata tcaacgagca gttttgcaaa attatcaatc ccaatttgga
     2821 tgagcggcca gataaagccc aaaaaaacag ctgcgattgt ttgtagaatc atcaccatag
     2881 tcaatgggaa tttttcgccc gcaaaataag aaaaaactac tggtaatttt tgttctttaa
     2941 agcgattata cgtccatgcc gctactaaac cggaaataat tccaccaaaa atccccatat
     3001 tcacttctgg gtccataatt tctaagcctt ttttcaaggt agcaatcgct aaaaaaccag
     3061 taagtgcagg tattcctttg tccttatttt tgacaaaacc gattgcaact cctatagcaa
     3121 ataagatatc caagtttcca aaaacggcat ttccagctgc accaattact gggatgttca
     3181 gcatatcgtc agctgataaa cgcatgatca atcccgctaa tggcatcgct gcaactggaa
     3241 gtagcatcgc tcttccaagc ttctgcacat ttgatttaac attcattttt attcctcctc
     3301 tattaaatat cctcatatgt tactaactca atcaaaggat catttttcac tttttgagca
     3361 aatgctgaat gaattaaaaa ttcgacttgg tgggtacgag gatacacaaa acttgaacca
     3421 tttaacaatt cctgatccaa atacgctgcg tgacacatga cttcgatcgt tttatcttct
     3481 ctcaatatca tgagtaaact tgccaaatat tcttccacac tcgtattgat ttgtcgtttt
     3541 ttttcatcag tttctcctac gacatcgaaa caaggctcaa aataatcgac atgacgaact
     3601 tcatcttttc gttcaaagtt gctccgaacc ggcaaatcat atttttttgc taatgtaaca
     3661 actacctcct gattttcatc caaagtatga gtatgatgat gactgtctaa atgagttggt
     3721 atgatccccg catgataaat tttttgaatc tgagcattcc attcctgata cagttgatct
     3781 ttgtcaatgt taaatggatg ctgataataa gataaggatc tgaactctcc gctttcgata
     3841 agagtatcga cagttttgag gagtggtttg ccacacgtca atgttaaatg gactcctaca
     3901 cctagcctag gcatttcctt tcttagtttc acagcatgct cgaaacccgg catatttgcc
     3961 atcaatgtag ttgatgtcaa aattcctcgc tgataagcat ccataatccc ataattcaca
     4021 ccatggctat agccaaaatc atccgaatta aaaatcactt ttcccatttc attctccttc
     4081 ttttttctac taaaataggc gcaaccaaat aatcatagtc catttactat gtgtcatttg
     4141 gttgcgccta tagatagtta cgcccttatt ttaatagatt cttgctagat gaattgccaa
     4201 atagatgatt tcattttctt gaatggcaag ttgaaattgc tgttcgatga tatctttaat
     4261 ctttagtgct gtacaatacg ctttgctatg tttaaatgtt tcagtaaaaa aaccaatatt
     4321 ttctactggc gtttcttcct gataaatcaa gcgtttaacc aggaatttga tatgaaccat
     4381 taagcgatga taagtcagtg attctttatc ataaacgttt gaataggatt tttctagatg
     4441 ggacaaaatc gtggtcgtca tttctactat ttttaagctc tttaaactat taggctctcc
     4501 tagctcagca ttaatgatat gcatagtgat gaatcctact tcatttgacc ctaactcggt
     4561 ttgatatcga tgattgattc gctcgaccac ttctactgct atatcatatt catttggata
     4621 aaggaatctc atttctggca acataattgc tgtgacatct ctatcttctc tttttcgttg
     4681 aatagcaaaa taaatatgat ctgtcaatgt aatatagata tttgaatcta atggagagga
     4741 gatcctagct gttatcagtt caatcacttc ttccgcaaaa tccataaatt cgattggaat
     4801 ctgcgagact atctcactga atcgtttgat cttttcttta gaatctaata caaataattt
     4861 gtcagcatcc tcaacatcaa atggcttact cactttagca gaaaagccaa tccctttccc
     4921 catcgcaatt ttttctattc catcgtcatc gatcaataca gcattatgat tcagcttttt
     4981 catcacatac accgttaatt cacctcctct atatgataaa acaaaacaac ttgcagactc
     5041 cttcatacat atttttgtct ccaaacaatt tttaaagaca aaaaacgacc taagaaagaa
     5101 ccctttctta ggtcgtgcct aattgaatag tagcacccta cataatttac aaaaatagtt
     5161 taacacataa taaaagcgtt atcaatatct tttttactcg ttttatgata atacttgtat
     5221 cgaattgatt tggaggtaat tacataaaaa caaaaaaaat atagcaactc tctataagaa
     5281 agttgctata ttgataatta taccggcggc cggggtcgaa ccggcacgtc ctcaacggac
     5341 actggatttt gagtccagcg cgtctgccaa ttccgccacg ccggcaaatt taaaaataat
     5401 aactggggta gctggattcg aaccaacgca tgagggagtc aaagtccctt gccttaccgc
     5461 ttggctatac cccaagaata aagggcgaat gatgggaatc gaacccacga atgccggagc
     5521 cacaatccgg tgcgttaacc acttcgccac attcgccatc ataaaagatg ttatttaatt
     5581 tctcaataaa aaactaagat gaagacatct tagtcaatac cggcggccgg ggtcgaaccg
     5641 gcacgccctc aacgggcact ggattttgag tccagcgcgt ctgccaattc cgccacgccg
     5701 gcaagttgat ttattagtac taaggcggta accggatttg aaccggtgat gaaggttttg
     5761 cagacctctg ccttaccact tggctatacc gccattatac tattatcaat ctataaaata
     5821 aagaataaat tatagaaact ggggtagctg gattcgaacc aacgcatgag ggagtcaaag
     5881 tcccttgcct taccgcttgg ctatacccca agaataaaaa gggcgaatga tgggaatcga
     5941 acccacgaat gccggagcca caatccggtg cgttaaccac ttcgccacat tcgccatggc
     6001 aggggcagca ggaattgaac ccacactaac ggttttggag accgctgttc tacctttaaa
     6061 ctatgcccct ataaaatgga ggagagtgga ttcgaaccac tgaaccctaa ggaacggatt
     6121 tacagtccgt cgcgtttagc cacttcgcta ctcctccatg gtggcgcggg acagaatcga
     6181 actgccgaca cacggagctt caatccgttg ctctaccaac tgagctaccg cgccatattt
     6241 taaaattatt aattgaataa caagaacgac ggttccgacg ggaatcgaac ccgcgatctc
     6301 ctgcgtgaca ggcaggcatg ttaaccccta caccacggaa ccagttcatt atggaggtta
     6361 acgggatcga accgctgacc ctctgcttgt aaggcagatg ctctcccagc tgagctaaac
     6421 ctccaacatt caattaaaat gacccgtacg ggattcgaac ccgtgttacc gccgtgaaag
     6481 ggcggtgtct taaccgcttg accaacgggc caggtaaatt tgctacggag agtaagggat
     6541 tcgaaccctt gagacagttt ggcaccatct acatgatttc caatcatgct ccttcggcct
     6601 ctcggacaac tctccagagt gaaagaagct caatactctt tctcaaaatg agaactccgg
     6661 cagtaggact cgaacctacg acatcatgat taacagtcat gcgctactac caactgagct
     6721 atgccggaat aacatcagcg tggcgacgtc ctactctcac aaggggcaac ccctcactac
     6781 aatcggcgct aagaagctta acttctgtgt tcggcatggt tacaggtgta tccttctcgc
     6841 tatcgccacc acactgtggt gttatctttt attgagtaaa ttttgttcac tcaaaactgg
     6901 atttgaagtc acataagttt ttttccgagt
//
LOCUS       DARBET010000105         6570 bp    DNA     linear   BCT 07-NOV-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4027
            SAMEA114569841-rid20112183.denovo.109, whole genome shotgun
            sequence.
ACCESSION   DARBET010000105 DARBET010000000
VERSION     DARBET010000105.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA114569841
            Sequence Read Archive: ERR12203666
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 6570)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6570)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001972618.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001972618.1
            Genome Coverage       :: 132x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/07/2023 13:18:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,041
            CDSs (total)                      :: 2,975
            Genes (coding)                    :: 2,908
            CDSs (with protein)               :: 2,908
            Genes (RNA)                       :: 66
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 67
            CDSs (without protein)            :: 67
            Pseudo Genes (ambiguous residues) :: 0 of 67
            Pseudo Genes (frameshifted)       :: 26 of 67
            Pseudo Genes (incomplete)         :: 28 of 67
            Pseudo Genes (internal stop)      :: 20 of 67
            Pseudo Genes (multiple problems)  :: 6 of 67
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6570
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA114569841-rid20112183.denovo.109"
                     /isolate="VRE4027"
                     /db_xref="taxon:1352"
                     /country="Denmark"
     gene            127..579
                     /locus_tag="SCJ86_002604"
     CDS             127..579
                     /locus_tag="SCJ86_002604"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000751236.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEG1170092.1"
                     /translation="MKKTILSIFSVFTILIGGYFLLNLDSVNSNAQIKDQRVAEHNNK
                     KMSLTKNIYSMSDEELYKEFGDLSGEQLEESIPGISDSDKIVVLSAGGQLMGDGKSEL
                     VSYKQNHRQFIDVNTDSNAISIKDLKELLNSKRNELKQIKETNSVELE"
     gene            593..1282
                     /locus_tag="SCJ86_002605"
     CDS             593..1282
                     /locus_tag="SCJ86_002605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000718009.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEG1170093.1"
                     /translation="MKKIFGVSVGLLMMLTFGNAVYAAEKEVISDPEQYASRYFADFE
                     EEDKVLALPEGGFLKGEAKIVSMDNPDIIQGSYNSETDPASVSVTEAKKIVAEKFKND
                     IETTPIMPRGAFPPDVGTSGHLLVLAGGGSYKSQPFSGAGWRFGGMGVKPADGTGNFL
                     RWKSYIDDGRVGNVNQANATKSGSIQGMALYAREGFKYFAPNGPGWTMNIGIYYTYNP
                     VAGTYYVVQNI"
     gene            1407..3758
                     /locus_tag="SCJ86_002606"
     CDS             1407..3758
                     /locus_tag="SCJ86_002606"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000344083.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1906 domain-containing protein"
                     /protein_id="HEG1170094.1"
                     /translation="MDEMVLSTQKWLNKKYSNVTGFDKVPENGRTGWPTIYGLIEGLQ
                     VELGITNLVANFGPTTEKMYDNQVTPKWGKNLPKNIVFLIQGAFWCKGINPGGFDGVY
                     TPYLDTAVKELQTDAGFNGSAVTGVLDAKWAKALFDMSAFVLVPGGDSKIRKMQQWLN
                     VNYLEYTGIMPCDGIYQRNSNQALIFALQAELGYSPSEATGSYGNGTTSKTYPVSEGN
                     SGNYVRIIQYGLYVNGYFEDGTFDGIFTKYMGLEVLAFRKFMVLPEYTEIADVVVIKG
                     LLSSAGDIRRSADGADTSTQLTRSQIQTLVDNDIKIVGRYLTGTVGIGKDERNKYLTT
                     EELNNIFSKNLSVFPIYQDGGAALAYFTYDRGLSDGKKAIDAAKNLNIPLTTVIYFAV
                     DLDMLGEEILAYAGEYFKGVSAVMSYSGYQTGVYGTRNVCSQIINNGYASFAFVSDMS
                     TGYSGNLGFPMPRQWSFDQFVEFTIGSGNGAVGIDLIATSGRDSGFNELSNDTNNDNY
                     IAKYNQKVINQMVRAYQYLSQAGLDNPWNPHLTFYRYVNYSGLSWDIISSPVTEHDRK
                     IYDEYRNKLTNSEGLYNYFIDPNTGSIIGLPHLIVTLQSQMFLADTINDSVSDFAGWL
                     GDLMTCWGEVKKLGIQMEQGVFALVGTAAGGTFSLEDLYQDVDAVNVYTLAKRNSDST
                     ALSALKTYYCDKGAIDRFNQFVKNRFGSSDKIASVTNSLLENDETVDILGVGIISFFI
                     DKIVNNSLGTDFTYLDFIGDEGKVANGFSEKIIQFQENRSKLN"
     gene            3838..4233
                     /locus_tag="SCJ86_002607"
     CDS             3838..4233
                     /locus_tag="SCJ86_002607"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304891.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEG1170095.1"
                     /translation="MMIMEEIEWNNLTQNNKNNSGCGIIFMFVIVLGIVVLLITVKIV
                     DYFANTPEEQIQRKLPEFERVPPYDPRDNSFQVKKLKESPNEMWFEVTVESSDFKEKK
                     KEYNYIVKAKVSKRFIFNPKLEIYSVVIE"
     gene            4558..5175
                     /locus_tag="SCJ86_002608"
     CDS             4558..5175
                     /locus_tag="SCJ86_002608"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001224538.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HEG1170096.1"
                     /translation="MRKFFYIRVSSYDQSIERQLISAKELGIPEEYIFVEKASGKDFK
                     RPEYQLMKRMFRKGDLLYLQSLDRLGRNKQMILEEWRELTKEKQIDISVLDMPLLDTA
                     SYKQLDGLETLISDLILQLLSYMAEDERKRINSRQREGVEAAKQKGVKFGRKKIALPP
                     EFPAIYSDWKAGKITAVYAMDQLGMKRNTFYRRVKEYEHTLHHFS"
     gene            5216..5515
                     /locus_tag="SCJ86_002609"
     CDS             5216..5515
                     /locus_tag="SCJ86_002609"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000366408.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BRCT domain-containing protein"
                     /protein_id="HEG1170097.1"
                     /translation="MDLTNQRVVFTGTLQQMTRTQAIGLVHSLNGHCQSSVTSKTNFL
                     VCGQYSKSLFDDSLYTKKEQTARDLIALGHEITILSEVEFYALISQQLKEWQRYL"
     gene            5549..5716
                     /locus_tag="SCJ86_002610"
     CDS             5549..5716
                     /locus_tag="SCJ86_002610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000824191.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HEG1170098.1"
                     /translation="MKRKSYDRWGIEITSFNRNIAFHVECDCGELAKSVYQKNYFYCP
                     KCHRRYGVHGT"
     gene            complement(5761..6366)
                     /locus_tag="SCJ86_002611"
     CDS             complement(5761..6366)
                     /locus_tag="SCJ86_002611"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HEG1170099.1"
                     /translation="MGKIGYIRVSSQDQNLDRQLAMMEGLNIDKLFQEKASGKDTERP
                     EFQKLLNYIREGDCVIVTSLDRLGRDYEDIKNTVAFMKQKKVALKILDAQFLDFNTGN
                     ELLDTAMFDMFLSLLSYIAQNEREKIRERQRQGVLLAQKAGRYKGRPIEYSINSTDPQ
                     KRLVFKTVVDMLKQGIPVAEIAKENGISRPTIYKIKKANDL"
BASE COUNT         2265 a          910 c         1285 g         2110 t
ORIGIN      
        1 attcctcctg ctatcttttt ctattattct atcttatttg atagtagatt taaaactttg
       61 caacagaacc aattatctta tcgtagatat atattataaa tttaataaga aagcgaggtg
      121 agaatcgtga aaaaaacgat actaagtatt ttttcagtat ttacaatatt aattgggggc
      181 tattttcttt tgaatttaga ttctgttaat tcaaacgctc aaataaaaga tcaaagagta
      241 gctgaacata ataataaaaa aatgagtttg accaaaaata tctattctat gtcagatgaa
      301 gaattgtata aagaatttgg tgatttatcc ggggaacagt tagaagaaag tattccagga
      361 attagtgact cagataaaat tgttgtcttg tctgctggtg ggcaattaat gggagatggc
      421 aaatccgaat tagttagtta taaacaaaat cacagacagt ttattgatgt taatactgat
      481 tccaacgcta tttcgattaa ggatctaaaa gagcttttaa atagtaaaag aaatgaatta
      541 aagcagataa aagaaactaa ttcagtggaa ttagagtaaa ggaagattca ttatgaaaaa
      601 gatttttggt gtttctgttg ggctacttat gatgttaaca tttggaaatg cagtatatgc
      661 tgctgagaaa gaagttattt cagatccaga gcagtacgcc tcacgatact ttgctgattt
      721 tgaagaagaa gataaagtac ttgcattgcc agaaggtggt tttttaaaag gggaagctaa
      781 aattgtatct atggataatc cagatataat tcaaggttca tataattcag aaactgatcc
      841 tgcttcagtt tcagtgacag aagcaaaaaa aattgttgct gaaaaattca aaaatgatat
      901 tgaaactact ccaattatgc ctcgaggagc atttccaccc gatgttggta ctagcggaca
      961 ccttcttgta ctagcaggag gaggaagcta taaatcacag ccatttagtg gtgcgggatg
     1021 gcgttttgga ggtatgggtg taaaaccagc tgatggtaca ggcaactttt tacgttggaa
     1081 atcctacatt gacgatggaa gagtaggaaa tgtaaatcaa gccaacgcaa ctaaatctgg
     1141 ttctattcaa ggaatggcat tgtatgctcg tgaaggattc aaatactttg caccaaacgg
     1201 gcctggttgg acaatgaata taggtattta ttacacttat aatcctgttg caggtacgta
     1261 ttatgttgtt caaaatattt aataaaacag cacaaagaga tttctttgtg ctgttttatt
     1321 atttttagtt gttgacaaac actaaaatat aaaatataat caacacataa acgaacgtat
     1381 gttcggcaag aaagcagggg atttttatgg atgagatggt tttaagtact cagaaatggc
     1441 taaacaagaa atacagtaat gttacagggt ttgataaggt tccagaaaat gggcgaacag
     1501 ggtggcctac aatctatgga cttattgaag gactacaagt agaacttggt attactaatc
     1561 tagtagctaa ttttggacct accactgaaa aaatgtacga taatcaggtt actcctaaat
     1621 ggggcaaaaa tctacctaaa aatattgtat ttcttattca aggtgccttt tggtgtaagg
     1681 ggattaatcc aggtggcttc gatggggtat atactccata tttagatact gctgtaaaag
     1741 agttgcagac tgatgcaggg tttaatggaa gcgctgtaac tggagttttg gatgcgaaat
     1801 gggccaaagc gttatttgat atgagtgctt ttgttcttgt tcccggcgga gatagtaaaa
     1861 ttagaaaaat gcagcaatgg ttaaatgtaa attatttaga atacacggga ataatgcctt
     1921 gtgatggtat ttatcaaaga aattcaaatc aagcattaat ttttgcatta caagctgaac
     1981 taggatattc tcccagtgaa gcaacagggt cttatggtaa tgggacaact agtaaaactt
     2041 atcctgtgtc tgaaggtaac tcgggaaact atgtaagaat aattcaatat ggattatatg
     2101 ttaatggtta ttttgaagac ggtacattcg atggtatttt cactaaatat atgggtttag
     2161 aagtactggc attccgaaaa tttatggtat tgcctgaata tactgaaata gcagatgttg
     2221 ttgtaataaa aggactattg tctagcgctg gagatattag aagatctgca gatggcgcag
     2281 atacttctac gcaattaact agatctcaaa tccaaacatt ggttgataat gatataaaaa
     2341 tagttggtag atatcttact ggtactgttg gaattggaaa agatgaacga aataagtatc
     2401 taactacaga ggaattgaat aatatttttt ctaaaaattt atctgtgttt cctatctatc
     2461 aagatggagg tgcagcatta gcttatttta cttatgatcg tggcttatca gatggtaaga
     2521 aggctataga tgcagcaaaa aatctaaata taccattaac aacagttatt tattttgctg
     2581 ttgatctaga catgttaggg gaagaaattc ttgcatatgc tggagaatat tttaaggggg
     2641 tatctgctgt aatgtcatat agcgggtatc aaacgggtgt ttacggaaca agaaatgttt
     2701 gttctcaaat aatcaataac gggtatgctt cgtttgcatt tgtttcagat atgtcgactg
     2761 gttatagtgg gaatttaggt tttcctatgc ctagacaatg gtcttttgat caatttgttg
     2821 aatttaccat aggaagtggc aacggagctg ttggtatcga tttaatcgct acatcgggta
     2881 gagatagcgg atttaatgag ttatcaaatg atactaataa tgataattac attgccaaat
     2941 acaatcagaa ggttataaat cagatggtac gtgcgtatca atatctatca caagctggtc
     3001 ttgataatcc ttggaaccct catttgacat tttatagata tgtaaattat tctggtttgt
     3061 catgggacat catttcttct cctgtaacag agcatgatag aaaaatttat gatgaatata
     3121 ggaataaatt aactaatagc gagggcctgt ataattattt tatagatcct aatactggtt
     3181 caataatagg tttgcctcac ttaattgtga cgttacaatc acagatgttt ctagccgaca
     3241 caatcaatga tagtgtttct gactttgcag gttggttagg agatttaatg acttgctggg
     3301 gtgaagttaa gaaattagga atacaaatgg agcaaggtgt atttgcatta gtaggtacgg
     3361 ctgcaggtgg aacatttagt ctagaagatt tataccaaga tgttgacgcg gtaaatgttt
     3421 ataccttagc aaaaagaaat tctgattcaa cagctttatc ggctttaaaa acgtactatt
     3481 gtgataaggg tgctattgat agatttaatc aatttgttaa gaacagattt ggttcttcag
     3541 ataaaatcgc ttcagtaaca aatagtttat tagaaaatga cgaaacagtt gatatcttag
     3601 gtgtagggat aatttcattt ttcattgata aaatagttaa taattcactt gggacagatt
     3661 ttacttatct tgattttatt ggagatgagg ggaaagttgc aaatggattt tctgaaaaaa
     3721 ttattcaatt tcaagaaaat agaagtaaac ttaattaaga atcgtttact atttaaacag
     3781 tgtccttttc ctttttaaat atatacgata aaataagtat atgaattgta aaaggacatg
     3841 atgattatgg aagaaataga atggaataat ctgactcaaa ataataaaaa taattctgga
     3901 tgtggaatta tctttatgtt tgttatagtt ttgggaatag tagtactttt aataactgtc
     3961 aagatagtag attactttgc gaatactcct gaagaacaaa tacagagaaa acttcctgag
     4021 tttgaaagag taccacctta tgatccaaga gataattcat ttcaagtaaa aaaattaaaa
     4081 gagtctccaa atgaaatgtg gtttgaagtt acagttgaat caagcgactt caaagaaaaa
     4141 aagaaagaat acaattatat tgtaaaggca aaagtatcaa aaagatttat ttttaaccct
     4201 aaattagaaa tatatagtgt tgtaatagaa taatagtgtt aaaaaaattg taaaagttaa
     4261 agaagtcaaa ttctgatgtg aaaaataaaa tataccatgg aagacataaa agaaaaactg
     4321 gataactagt ttatttaatg gcagaccgtt aaggcctgcc atttttatgt attttagcgg
     4381 aataaatata atcatgcggg agtgtaaact tactgtttca aaaagtatac tttttgacat
     4441 gccttgattt tctttgttta tgtatggcat aataaccaca taatacaatt tgaaacgttt
     4501 ctgatttaat ctgattgttt tgaaacaatt ttagtgaaaa aagggtagat aaaaacgatg
     4561 agaaaatttt tttatatccg ggtcagtagt tatgatcaat cgatcgaacg acaactgatt
     4621 tccgcgaagg aattaggaat ccctgaagaa tatatatttg tagaaaaagc cagcggcaaa
     4681 gattttaaac gccctgaata tcaattaatg aaacggatgt ttcgaaaagg agatttactc
     4741 tatctacaat cattagatag actaggcaga aataaacaaa tgattttaga agaatggcga
     4801 gaattaacta aggaaaagca gattgatatt tctgtgttag acatgcctct gttagatact
     4861 gctagttaca agcaactaga tgggttagag acgcttatct ctgacttaat tctgcaatta
     4921 ctcagctata tggccgaaga cgagcggaaa cgaattaata gccggcaaag agaaggcgta
     4981 gaagctgcga aacaaaaggg tgtaaaattc ggcagaaaga aaattgcttt acctcctgaa
     5041 tttcctgcga tctattctga ctggaaagca ggaaagataa ctgcagtcta tgctatggat
     5101 caattgggca tgaaacgaaa taccttttat cgacgggtga aagaatatga acataccttg
     5161 caccactttt cttgatagat ataacagcta ttagacggga agggagagtt tgaaaatgga
     5221 cttaacgaat caaagggtcg tttttactgg aacgctgcag caaatgacac gaacccaagc
     5281 catcggtttg gttcactctt taaacggcca ttgtcaatca agtgttactt caaaaacaaa
     5341 tttcctggtt tgtggtcagt attctaagtc gttgtttgat gattctctct atacaaaaaa
     5401 ggaacaaacc gcgcgggact tgattgcgtt aggtcatgag attaccattt tatcagaagt
     5461 cgaattttat gcgttaatta gtcaacagtt gaaagaatgg caacggtatt tataatcgtt
     5521 agtaaaattt acaaggagga aaatttttat gaagcgaaaa tcttatgatc gttggggcat
     5581 tgaaataact agttttaatc gaaatattgc ttttcacgtg gagtgtgact gtggggaatt
     5641 agcaaaaagt gtttatcaaa aaaattactt ttactgtcct aaatgccatc gccgatacgg
     5701 ggtacatgga acatagaaac gaaatagtgc ggtagaaaag cgaatgaatg tagtatattt
     5761 ctacaaatca ttcgctttct ttattttata aatagttggt cgagagatcc cgttttcttt
     5821 tgcaatctct gcaactggaa tgccttgctt taacatgtcc accacggttt taaaaactaa
     5881 gcgtttttgc ggatctgttg agttaataga atactcgatt ggtcgtccct tatatcggcc
     5941 ggctttttga gccaataata ctccttgccg ctgacgttca cgaattttct cacgctcgtt
     6001 ttgtgcaata taacttagca gtgataaaaa catatcgaac atagcggtat ccaggagttc
     6061 atttccagta ttaaaatcta aaaactgtgc atctaatatt tttaacgcta cttttttctg
     6121 tttcataaaa gcaactgtat tttttatatc ttcataatct cttcctaaac gatctagtga
     6181 tgtaacgatg acgcaatcac cttcacggat ataatttagt aatttttgaa actcaggacg
     6241 ctcagtatct ttaccactgg ctttttcttg gaataattta tcaatgttaa gtccttccat
     6301 cattgccaat tgtcgatcca aattttgatc ttgtgaagat actcgaatat agccaatttt
     6361 tcccataaaa aactcctttt taaaagtgat ataatttaca tgtaaaattt acttgtagtt
     6421 ttactgctat catagcattt ttaaaaataa attttatgta gacttaaaat ttacttcatg
     6481 gagggtttaa atggatgaaa tcaaaagtaa caaaaaagca tttggcattt atttaggaga
     6541 acctagtaca gaacaattaa accaattttt
//
LOCUS       DARBET010000106         6551 bp    DNA     linear   BCT 07-NOV-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4027
            SAMEA114569841-rid20112183.denovo.110, whole genome shotgun
            sequence.
ACCESSION   DARBET010000106 DARBET010000000
VERSION     DARBET010000106.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA114569841
            Sequence Read Archive: ERR12203666
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 6551)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6551)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001972618.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001972618.1
            Genome Coverage       :: 132x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/07/2023 13:18:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,041
            CDSs (total)                      :: 2,975
            Genes (coding)                    :: 2,908
            CDSs (with protein)               :: 2,908
            Genes (RNA)                       :: 66
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 67
            CDSs (without protein)            :: 67
            Pseudo Genes (ambiguous residues) :: 0 of 67
            Pseudo Genes (frameshifted)       :: 26 of 67
            Pseudo Genes (incomplete)         :: 28 of 67
            Pseudo Genes (internal stop)      :: 20 of 67
            Pseudo Genes (multiple problems)  :: 6 of 67
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6551
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA114569841-rid20112183.denovo.110"
                     /isolate="VRE4027"
                     /db_xref="taxon:1352"
                     /country="Denmark"
     gene            42..2195
                     /locus_tag="SCJ86_002612"
     CDS             42..2195
                     /locus_tag="SCJ86_002612"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318500.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide cleavage/export ABC transporter"
                     /protein_id="HEG1170100.1"
                     /translation="MIKSFPFVQQQDEKDCGVACLSMILKYFKTDIPIHKLRDLSGTD
                     LEGTSAFGLKKTFEKLSFDCMAIQADSEIWKERDLILPLIAHILVDNSYMHYVVIYKT
                     KGNVLWIADPANGKNRKTIEAFQKEWTGILLLPMPRETYIPIKEKVAGLSSFLPIIWK
                     QKGLVFHIVLASIFITFFGIGSSYYFQGILDFFIPNQARSTLNIISIGLIAVYLFRVL
                     FEYSRSYLLIILGQRMSMAIMLQYFKHVLTLPMNFFATRKSGEIISRFLDASKIIDAL
                     ASATLSIFLDIGMVLLVGVTLAIQNSTLFLITLCSLPFYVVAIFAFVKSYEKSNREEM
                     AAGAAVNSSIIESLKGIETIKAYSGEEKVYNRIDHEFVKLMKKSFRTVTLDNIQQGIK
                     HLIQLISSALILWLGSYFVMDNRISLGQLITYNALLVFLTDPLQNIINLQVKMQTAQV
                     ANNRLNEIFAIDSEKSNQSFEYMLSEQLFQQDITVKNVSFSYSMKAPTLNEISCTIPS
                     HSKTAIVGVSGSGKSTLAKLFVRFYEASEGSIHYGNINHLDIPFQILRDRVTYLPQES
                     FFFSGTIIENLIFGLDSPPSFEQIIEVCEIVQLNEFINQQPLRYETLLEEGGNNLSGG
                     QRQRLALARALLKNPDILILDEATSGLDTLLEHAIMNYLLNLKEKTIIFIAHHLPIAK
                     ACDQILVLHEGHLVEKGTHDELRFADGIYQKLWEI"
     gene            2164..3579
                     /locus_tag="SCJ86_002613"
     CDS             2164..3579
                     /locus_tag="SCJ86_002613"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629565.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HlyD family secretion protein"
                     /protein_id="HEG1170101.1"
                     /translation="MVSIKNYGKSRRGKQFMQNNNWFEHSSIYTKQHHSFYRWVLYPV
                     ILFLCIIGLFLTFAKKEVVIRTTAQLNPEKIEKLQVPLEAKIMENFLKENQFVHKDDV
                     LVRLDCSLIENEKAQIEQENQRITQQIKMAQLFIESISKGKNLFSTDDSFGYSNQLKS
                     MLSEKESLRYALKQSELNDQKQLEVYEKTKRQLEKQIESSDSKLQEWQQVQVAWSNNQ
                     SLKDFSKEMMANYENWQEQLNNVSDDQKNQVKLTISASINEQIEQLKKEVEQYQSEKA
                     KLVKPTTSENDRISQTEKGKQELEQAITTVKQTVTELNEKQEKNQSVIKSLDDQLSKG
                     ILKAPVSGTVHLNEETKGQAELAKGTVLAEIYPIHEKSKMKFTALLPANESTYIKEGM
                     KVHFKLDQKGGAPITIDGTLDEISENSTATEKGVFYAVKGTLKTTQSFPFRYGLTGEL
                     SLIVGEKTYWQQIVDVFLNKK"
     gene            3612..4646
                     /gene="sppA"
                     /locus_tag="SCJ86_002614"
     CDS             3612..4646
                     /gene="sppA"
                     /locus_tag="SCJ86_002614"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptide peptidase SppA"
                     /protein_id="HEG1170102.1"
                     /translation="MNKRRWIAVGVACGLLLLSIIVALIPGKDKEEASNTTLTGINKI
                     FYGSNEITEETLEEGASNKKIVKLSVNGVIADTGESNLFSREQYNHQNFLTQLKKIQE
                     DKAVKGVLLEVNSPGGGIYESAEIAKEMAKIKKLDIPIYTAFKNTAASGGYYISAGSD
                     KIFATEETTTGSIGVIISGLNYSGLLEKLGVTDATYKSGALKDMMSPQHKPSEEENKV
                     IQEFVMSAYDRFVNVVAKGRNMDTNAVKELADGRIYDGNQAVENGLVDQIGYSEDALD
                     SLKKEKKLTDATVIEYKNDTTGFASSWLGNKIAEWQGLKATTSDRVISVFEKLGFSDT
                     PKPMYYYGGL"
     gene            4648..5169
                     /locus_tag="SCJ86_002615"
     CDS             4648..5169
                     /locus_tag="SCJ86_002615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294608.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RDD family protein"
                     /protein_id="HEG1170103.1"
                     /translation="MNKKSGIETLPNYVFAGFWLRFFAFLIDLICISIITHATIGLFY
                     KIMGLESDKSIITFYGIASLAIYLTYFVLLTKLNSGQTIGKMIFGIRVVSFEEKELSW
                     QTIFIRECICRFILKNFVFQIGYLVAAFTPKKQHIGDYFSNTSVVVIRSLEAINYGQK
                     ISNSKTLNLERIK"
     gene            5166..5552
                     /locus_tag="SCJ86_002616"
     CDS             5166..5552
                     /locus_tag="SCJ86_002616"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4064 domain-containing protein"
                     /protein_id="HEG1170104.1"
                     /translation="MNKRKTEFILILISGITGILCSLLALMGGMFLSSIDSTTLNTTE
                     LTSKDIDMLVNLSGNVVLFGGITLVFSIMIFIIGFLVRKNQKTVVYGISLLILSIIPF
                     FLLTLLWVIPGVLGIIAGLMLLIRKS"
BASE COUNT         2353 a          885 c         1208 g         2105 t
ORIGIN      
        1 tttttctata aaaatttttt agaaggagtt gtgttagata agtgataaaa tcatttcctt
       61 ttgtgcagca acaagatgaa aaagattgtg gtgttgcttg cctttcgatg attttaaaat
      121 attttaaaac tgatatcccg attcataaat tacgggacct atctgggact gatttagagg
      181 gtacttcagc atttggattg aaaaagacat ttgaaaaatt gtcttttgat tgtatggcta
      241 ttcaagcaga tagtgagatc tggaaagaaa gagatcttat cttaccatta attgcacata
      301 tattggttga taacagctat atgcactacg ttgtaattta taaaacgaag ggaaacgtac
      361 tatggattgc agatcccgct aatggaaaaa atagaaaaac gattgaagcg tttcaaaaag
      421 aatggacagg tattctttta ttacctatgc ctagagaaac ttatattcct attaaagaaa
      481 aagtagctgg attaagttct tttttgccta taatttggaa acaaaaagga ttagtattcc
      541 acatcgtttt agcatcgatt ttcattacgt tttttggcat aggaagctca tattattttc
      601 aaggtattct tgattttttc atccctaatc aagcacgttc aactttaaat ataatttcca
      661 ttggcttgat cgctgtctat ttatttcggg tattgtttga atatagtcgt agttatcttc
      721 ttattatttt aggacagcga atgagtatgg caattatgct tcagtatttc aaacatgtgt
      781 taactttacc aatgaatttt tttgctacga gaaaatcagg ggaaattatt tctcgttttc
      841 ttgatgccag taagattatt gatgctttag caagtgctac gttatcaata tttttagata
      901 ttggtatggt gttgcttgtt ggggttacgt tagctattca gaatagtacg ttgtttttaa
      961 tcactctatg ctctttgcct ttttatgtag ttgcaatatt cgctttcgtg aaaagctatg
     1021 aaaaatcgaa cagggaagaa atggcagccg gtgcagctgt aaattctagt atcatagaaa
     1081 gtcttaaagg aatagaaaca attaaagcat atagcggtga agagaaagta tataaccgta
     1141 tagaccatga atttgttaaa ttaatgaaaa aatcatttcg tacggttacg cttgataata
     1201 ttcaacaagg aattaaacat ttgattcaat taattagcag tgcattaatt ttatggctag
     1261 gttcttattt tgttatggat aatagaatta gtctaggtca acttatcaca tacaatgctt
     1321 tactagtatt tttgacagat cctttacaaa acattattaa tttacaagtg aaaatgcaaa
     1381 cggctcaagt tgcaaataat cgtttaaatg aaattttcgc gattgattca gaaaaatcga
     1441 accagtcttt tgaatatatg ctttctgaac aattatttca acaagatatc actgtaaaaa
     1501 acgtttcatt ctcgtatagc atgaaggccc caacgttgaa cgaaatttca tgtacaattc
     1561 cttcgcacag taaaacggct atagttggag ttagtggttc tggaaaatca actcttgcaa
     1621 agttatttgt tcgtttttat gaagcatcag aaggatcgat ccattatgga aacattaatc
     1681 acttagacat tccttttcag atattacgcg atcgagtgac atatttgcct caagagtcat
     1741 ttttctttag tggaacaatc attgagaatc ttatattcgg tttagatagc ccaccaagtt
     1801 tcgaacaaat aatagaagtt tgtgagatag tccaactaaa tgaatttatt aatcaacagc
     1861 cattacgtta cgagacattg ttggaagaag gtggaaataa tctttcagga gggcaacgac
     1921 aacgattagc cttagcacgg gctttattaa aaaatccgga catattaatt ttagatgaag
     1981 caactagtgg attagatacc ttgttagaac atgcaattat gaattatctc ctcaatttaa
     2041 aagaaaaaac gattattttt attgcgcatc atttaccaat agcaaaagca tgtgatcaaa
     2101 ttttagttct ccatgaaggg catttggtag aaaaaggaac gcacgatgaa cttcgttttg
     2161 ccgatggtat ctatcaaaaa ttatgggaaa tctaggagag ggaaacaatt tatgcagaat
     2221 aataattggt tcgaacattc tagtatctat actaaacaac atcatagctt ctaccgttgg
     2281 gtgttatacc ctgtaattct ttttttatgt attattggat tgtttttaac ctttgctaaa
     2341 aaagaggttg ttattcggac aacggctcaa ttgaaccccg aaaaaataga gaagttacaa
     2401 gtgccattag aagctaaaat aatggaaaac ttcctaaaag aaaatcaatt tgttcataag
     2461 gacgatgtgt tggttagatt ggattgttct ttaatagaga atgaaaaggc tcagatagaa
     2521 caagaaaacc aacgtattac tcaacaaata aagatggctc agctatttat tgaaagtata
     2581 agtaaaggaa aaaatttgtt ttcaacggat gacagttttg gctacagtaa tcaattaaag
     2641 agcatgttgt cagaaaaaga atcactccgc tacgctttga agcaaagtga attaaatgat
     2701 caaaagcaat tagaagtata cgaaaagaca aaaagacaac tagaaaaaca aattgagagt
     2761 tcagatagta aattacaaga atggcaacaa gtacaggtag cttggagtaa taatcaatca
     2821 ttaaaagatt tttcaaaaga aatgatggca aactatgaga attggcaaga acaactaaat
     2881 aatgtttctg atgatcaaaa aaatcaagtg aaactgacaa tttcagcaag cataaatgaa
     2941 caaattgagc aactaaaaaa agaagtagaa cagtatcagt cagaaaaagc taaattagtt
     3001 aaaccaacta cttctgagaa tgacagaatt agtcaaacgg aaaaaggaaa gcaagagcta
     3061 gaacaagcaa taacaactgt aaaacaaaca gttacagagc taaatgaaaa acaagagaag
     3121 aaccagtcag ttatcaaaag tttggacgat caactttcaa aaggaatact aaaagctcct
     3181 gtatctggaa cggttcattt aaatgaagaa acaaagggac aagcagaact agccaaagga
     3241 acagtgcttg cagaaattta tccgatccac gaaaaatcaa aaatgaaatt tacagcactt
     3301 ttacctgcga atgaaagtac ctatattaaa gaagggatga aggttcattt taaattggat
     3361 caaaaaggtg gggctccgat tacgatcgac ggaactttag atgaaatttc agaaaattct
     3421 actgccactg agaaaggtgt tttttatgca gtgaaaggaa cgttaaaaac aactcaatcg
     3481 tttccgtttc gttatggatt aactggagag ttgtctttaa ttgtaggaga aaaaacatat
     3541 tggcaacaaa ttgtcgatgt gtttctaaat aaaaaataat tatttttact aagaatatag
     3601 gaggagttta tatgaataaa agaagatgga ttgctgtggg agtagcgtgc ggattattgt
     3661 tactatccat tatcgtcgcg ctaattccag gaaaagataa agaggaagca tcaaatacaa
     3721 ccctaacagg aataaataag attttttacg gatctaacga aatcacagaa gaaacgttag
     3781 aagaaggcgc atcaaataaa aagattgtca aactatccgt caatggagtt attgcagata
     3841 caggtgaaag caatcttttt agtagggaac aatataacca tcagaatttt ttaacacagc
     3901 taaagaaaat tcaagaagac aaagcggtta agggagtgtt attagaagta aattcacctg
     3961 ggggaggaat atatgaaagt gctgagattg ctaaagaaat ggcaaaaatt aaaaagctag
     4021 atattcctat ctatactgca tttaaaaata cagcggcaag tggagggtac tacatttctg
     4081 ctggttcaga taaaatattt gctacggaag aaacaacaac tggatcaatt ggtgtcatta
     4141 tttctggttt aaattattct ggcttattag aaaaacttgg cgtaactgat gcgacgtata
     4201 aaagtggtgc attaaaagat atgatgtcac ctcaacacaa accttcagag gaagagaata
     4261 aagttataca ggaatttgtg atgagtgctt atgatcgttt tgtcaatgtt gtagcaaaag
     4321 gaagaaatat ggatactaat gcagtgaaag aacttgcgga tggaagaatt tatgatggta
     4381 accaagcagt agaaaatggg ttggtggatc aaattggtta ctctgaagat gcattagatt
     4441 ccctaaaaaa agagaagaaa ttaactgatg cgacggttat agaatataaa aatgacacaa
     4501 ctggctttgc ttcttcttgg ttaggtaata agattgctga atggcaaggg ctaaaggcaa
     4561 ctacatcaga tcgtgtaatt tcagtttttg aaaaacttgg attttctgat actccaaaac
     4621 caatgtatta ctatggaggg ctataaaatg aataagaaga gtggcattga aactttgcct
     4681 aattatgtat ttgcgggatt ttggttacga ttttttgctt ttcttattga tttaatttgt
     4741 attagcatca tcacccatgc tactataggt ctcttttaca aaatcatggg actggaatct
     4801 gacaaatcca ttattacttt ttatggtata gcgtcattag ctatttattt aacctatttt
     4861 gtacttttga ctaagctgaa ttcaggacaa acaattggga aaatgatttt tgggataaga
     4921 gtagtgagct ttgaggaaaa agagctaagt tggcaaacta tctttattcg tgagtgtata
     4981 tgtaggttta tattaaaaaa ttttgtattt caaattgggt atttggtggc tgctttcacg
     5041 cccaagaaac aacatattgg ggattatttt tctaacacgt cagtggttgt tattcgttca
     5101 ttagaagcta ttaactatgg tcaaaaaatt tctaattcta aaacacttaa tctggagaga
     5161 attaaatgaa taagagaaaa acagagttta ttttgatttt aattagtgga ataacaggga
     5221 ttttatgtag tcttttagca ttgatgggtg gtatgtttct atcttctatt gattcaacta
     5281 ctttaaatac tacagaacta accagtaagg atatagatat gttagtcaat ctaagcggga
     5341 atgttgtttt atttggagga attaccctag ttttttcgat aatgattttc ataataggtt
     5401 ttttagtaag aaaaaatcaa aaaacagtgg tctatggtat tagtttactc attttatcta
     5461 ttattccatt ttttctttta acattattgt gggtaattcc tggagtttta ggtattattg
     5521 cgggattgat gcttctaata agaaaatctt aattaggcaa tgtgtagaca acgtgaaacg
     5581 attaatcaat taaaagctaa atacgttttg agaaatgata acttgtagtc gatggaagac
     5641 atgtagataa aaaaactacg tgtcttttat ttattctaat tttttcgaaa aaaagacact
     5701 ctctgattgt tgtattaatg gaaaagggga aagcagcaag taaatttagt ttttctatta
     5761 aatattcttc actttttaac cttttatagt acttagattt aatcctatat gtttagagag
     5821 ttcattggca gcatgatttt gcaaagcttc tcgaattatt gttatattag aaactttact
     5881 gttcataatt atgcttcttt tttaactcaa agtccaaagg gctacagtgg actttgagtt
     5941 aaaaataaaa agccctagtt attttgaaaa ataaaaaaag aacagcgtaa atgttttcga
     6001 aatgctttcg caccaacatt tacctgttcc gtgggtttgg cacccaatgg gccgtgaggg
     6061 gctcgaaccc gcgacccgct gattaagagt cattaatgtc aatttttaaa taaaattaat
     6121 atgtctcttt aagtattaaa ctatcactct ttatcctatg ataaatcaat gttttttaat
     6181 attagtagtg atatattaac atcaaataat attaactaag aaagataagg aaagatagaa
     6241 atagggaaaa aaatcggaaa aaatgttggc aaaaattttg ataaatttac tataaaaata
     6301 acgtcgatgc tttaataagt atcgacgctt tttacattat aggaaatata ttatgaatgg
     6361 tgtttaaaaa ttaatagaat caaacttttg ggcacatttc acttgatctt tttctcttta
     6421 aaatgaataa atgaaggcga agattctaca atctaaatat tagtaagtgt ttagttttta
     6481 cccgaaacgt attttagaaa ttaaatatca acaagaagaa actctgtgta attcgattta
     6541 tacagagctt t
//
LOCUS       DARBET010000107         6303 bp    DNA     linear   BCT 07-NOV-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4027
            SAMEA114569841-rid20112183.denovo.111, whole genome shotgun
            sequence.
ACCESSION   DARBET010000107 DARBET010000000
VERSION     DARBET010000107.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA114569841
            Sequence Read Archive: ERR12203666
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 6303)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6303)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001972618.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001972618.1
            Genome Coverage       :: 132x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/07/2023 13:18:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,041
            CDSs (total)                      :: 2,975
            Genes (coding)                    :: 2,908
            CDSs (with protein)               :: 2,908
            Genes (RNA)                       :: 66
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 67
            CDSs (without protein)            :: 67
            Pseudo Genes (ambiguous residues) :: 0 of 67
            Pseudo Genes (frameshifted)       :: 26 of 67
            Pseudo Genes (incomplete)         :: 28 of 67
            Pseudo Genes (internal stop)      :: 20 of 67
            Pseudo Genes (multiple problems)  :: 6 of 67
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6303
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA114569841-rid20112183.denovo.111"
                     /isolate="VRE4027"
                     /db_xref="taxon:1352"
                     /country="Denmark"
     gene            334..714
                     /locus_tag="SCJ86_002617"
     CDS             334..714
                     /locus_tag="SCJ86_002617"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348702.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MobC family plasmid mobilization relaxosome
                     protein"
                     /protein_id="HEG1170105.1"
                     /translation="MSEQHQNLASDHSKKYTYRSEPKQISFRVSESEFAKLKQSAEAL
                     QMSVPAFVKAKAQNARLVTPKVAPDIAQAIARDLAKAGGNINQIAKWCNTHQHDVAPD
                     DAQRLSENLKIMQKELQKIWQQLK"
     gene            696..1610
                     /locus_tag="SCJ86_002618"
     CDS             696..1610
                     /locus_tag="SCJ86_002618"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340563.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="relaxase/mobilization nuclease domain-containing
                     protein"
                     /protein_id="HEG1170106.1"
                     /translation="MATVKVSRTTSCSRAINYAEPRATVKTGINCDIDYAKSEMKQIR
                     TLYGKDDRVQAHLLIQSFRPGEITAEKANQLGAEYAKKIAPNHQIAIYTHTDKDHIHN
                     HIVINSVNLETGKKFQAHGQAFLNKCYDLNDEICLEHDLTITERGKKTEKRTMSEIKL
                     KEKNEPVWKDEIRFAIDQTMKNKKTRTYTQFCDSLKTFGIHCLNRGKNFTYELVHKKK
                     KVRSNKLGKDYEKETILHELDRRERTYNQQRTNELRRARASIRETAEEVRREQRTFTP
                     EQSEIRPTRRNHEQHVERGTDHQQELSL"
     gene            1855..2544
                     /locus_tag="SCJ86_002619"
     CDS             1855..2544
                     /locus_tag="SCJ86_002619"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEG1170107.1"
                     /translation="MAFYLPYLLISASILGIIWLSYKAYQTRYSLKGSKIRFKRFLLV
                     GVIFALSIVLSGLFGVLEGNKRVSRSILLGDITQKYEAACDKKKKEQALAQKMEEFTA
                     CYEDMNNIFVDQEKRLTDKNMKKLTRFYQNLPEKQQKEVQEKYEQVKKDVQYFKDTQT
                     EEACCNLFSDTIPFSTSEQERKERQQSVTYERYETLLQQATNIQNPTKKETALNYLES
                     VKEWLDQQQQN"
     gene            complement(2665..2751)
                     /locus_tag="SCJ86_002620"
     CDS             complement(2665..2751)
                     /locus_tag="SCJ86_002620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000493114.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system Fst family toxin"
                     /protein_id="HEG1170108.1"
                     /translation="MFERFICPLLVEVCVSLFSYWLNNRNKK"
     gene            3427..4377
                     /locus_tag="SCJ86_002621"
     CDS             3427..4377
                     /locus_tag="SCJ86_002621"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RepB family plasmid replication initiator
                     protein"
                     /protein_id="HEG1170109.1"
                     /translation="MANEIVKYHHELNTIPLRKFTPVEMNLFFSIVSRMREKGDKTVR
                     LTFDQLKDLSNYKATANNRFINDLEMTYDKLMDLRFGKRSANGLDIDRFVMFSEFKIR
                     GTAKDPYVDIQVYEKALPLLNNLDEWVRYSLQQFNELQSSYAKTMFRLLKQYRTKGFA
                     YFSKEDFHELLDIPKSYWNNQANVDNRVIKPIRQELTAIFKGLSIKKKYGKGRGKPVV
                     GYQFTFKPEIKKADDFNKHAPTKEEQNKALALADKQKEYEEPATTINEKIKQKKRELE
                     LLQQIKQLEKEKEALEKQTESALSKKEKKSLLSDLDNLFQ"
     gene            4398..4931
                     /locus_tag="SCJ86_002622"
     CDS             4398..4931
                     /locus_tag="SCJ86_002622"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292473.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF536 domain-containing protein"
                     /protein_id="HEG1170110.1"
                     /translation="MQPQETFTIKELSELFKMSRQAISKHIQKLDSSMIAKNERGYKV
                     VLRSGVLQLARNLGNQMLLDRLHEYEQDNDKNVNIPSKNVVTNKLLDQLREENEFLRE
                     QLALREQNNSHIQKLLDQQQQLTLQANKQIEKLQEKLQLTYTEEAPENDSTTLSEATE
                     TIDKQIDTEKKWWQLWK"
     gene            4947..5117
                     /locus_tag="SCJ86_002623"
     CDS             4947..5117
                     /locus_tag="SCJ86_002623"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322958.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEG1170111.1"
                     /translation="MTRKNLLFGSILLGILGFSPMLLSVINKGNKHGELKLNPNYYNS
                     PNEELFVKETDK"
     gene            5567..6100
                     /locus_tag="SCJ86_002624"
     CDS             5567..6100
                     /locus_tag="SCJ86_002624"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334266.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEG1170112.1"
                     /translation="MKKFTLGVALLTIGAVVLPSTTIVYASENSQVVSLNENNATENV
                     VIEYTEDGMVVTKYEDGYSAEDFANSSDIMTRAAVARWGKKEYTNIAVTTGVAANAIN
                     AAFWAGGKASTSMFGIPGWAIGALLTGASWTKKGASPGNAVAKEWDKNGNGWIGFYYQ
                     RGYDAAGRVVATRYSTL"
BASE COUNT         2323 a         1102 c         1174 g         1704 t
ORIGIN      
        1 aaaaaaaaat aaatcgatac attttagaag aatagggttg tttgaatatt acaaagcaac
       61 aatttcacct tcggtgaaat aagcgaaact ttacaaaaag tggctagcaa atgctagcct
      121 ttttttgtat tgtggagcgt ggattttgag ggggattcaa gggggagctt gcgctccttt
      181 gacaagcggt tatttgcacc aaatacggag gtatttggct agcaaatata gagcttgcca
      241 aaccctttta gtgagcttta ctcattgtac ctttttaggt acaatggtat tgcgaacgat
      301 acgtccgtag gacgtactga aagggtggga cttatgagcg aacaacatca aaatttggct
      361 agcgatcatt ctaaaaaata tacctatcga tccgagccaa aacaaatcag ttttcgagtg
      421 agcgaatccg aatttgcaaa gctgaagcag tcagctgaag ctttgcaaat gagtgtgccg
      481 gctttcgtga aagccaaggc acaaaacgcg cgtctggtga caccgaaagt ggcgccagac
      541 attgcccagg caatcgcgcg cgatttagcc aaagctggag ggaatatcaa tcaaattgcc
      601 aagtggtgca atacacacca acacgacgtt gctcctgatg atgctcaacg tttatccgaa
      661 aacctaaaaa tcatgcaaaa ggagctacaa aagatatggc aacagttaaa gtaagtcgaa
      721 ccacttcatg tagccgagca atcaattacg cagaaccacg agcaaccgtc aaaacaggaa
      781 taaactgtga cattgattat gccaaaagtg aaatgaaaca aatacgcacg ctttatggaa
      841 aagacgatcg tgtacaagca cacttattga ttcagtcctt tcgaccagga gaaattaccg
      901 ctgaaaaagc caatcaacta ggggcagagt atgcgaaaaa aatagcccct aaccaccaaa
      961 tcgcgattta cacacataca gacaaagacc atattcataa tcacattgtc attaactccg
     1021 ttaatcttga aactgggaaa aaatttcaag cccatgggca agcattttta aacaaatgtt
     1081 atgacctaaa tgacgagatt tgtcttgaac atgacttaac aatcacggaa cgaggaaaga
     1141 aaaccgaaaa acgaaccatg tctgaaatta aattaaaaga aaaaaatgaa ccggtttgga
     1201 aagacgaaat tcggtttgcg attgatcaaa caatgaaaaa caaaaaaact cgaacgtata
     1261 cccaattttg tgattcactt aaaacattcg gcattcattg cttgaatcgc ggaaaaaatt
     1321 ttacttacga attggtccac aaaaagaaaa aagtacgatc caacaaacta ggaaaggatt
     1381 acgaaaagga gacgatttta catgagctgg acagacgaga acgaacctac aatcaacaac
     1441 gaaccaacga gctacgaaga gctcgagcta gcattcgcga aactgcagaa gaagtacgaa
     1501 gagaacaacg aacttttacg cctgaacaat cggaaattag accgactcga agaaaccatg
     1561 aacaacacgt tgaacgagga acagaccacc aacaagaatt atcgctctaa aacgttaaaa
     1621 gacatcgaaa acgaaataaa gagacaacgt gcctttcttg cgccttatac ccaagaaagg
     1681 atacaaacta cgatggattc acaagtcaaa aaactccacc aagcgctatt agaccagcga
     1741 aaccaagaac aaaaaaaggc tttaattcaa cgatcgatgg atattcttgc ccccttatgt
     1801 tgcttagtac taacattatt agccttttgg ttggtatggg gagaataaac agacatggca
     1861 ttctatttac cctatctact catttctgcc agtattttag gaattatttg gcttagctat
     1921 aaggcttatc aaacaagata ttcattaaaa ggctctaaaa ttcgatttaa gcgattttta
     1981 ttggtcgggg ttatatttgc cttatcaatc gttttaagtg gtttattcgg cgttttagag
     2041 ggtaataaac gtgtttcaag aagcatttta ttaggggata tcactcaaaa gtatgaagca
     2101 gcatgtgata aaaagaaaaa agagcaagca ttagcacaaa aaatggagga atttaccgct
     2161 tgttatgagg acatgaacaa tatttttgtg gatcaagaga aacgtttaac agataaaaac
     2221 atgaaaaaac tcactcgttt ttatcaaaac ttaccagaaa aacagcaaaa agaggtacaa
     2281 gaaaaatatg agcaagtaaa aaaagacgtg caatatttta aagatacgca gacagaagaa
     2341 gcttgttgta acttatttag cgatacaatc cctttttcaa ccagtgaaca agaacgaaaa
     2401 gaacggcaac aatcggtcac atacgaacgt tatgaaacac tgcttcagca agcaacaaac
     2461 atacaaaatc caacgaaaaa agaaacggca ttaaattatc tagaaagtgt caaggaatgg
     2521 ttggatcagc aacaacaaaa ttgaatttga ttagcaatca tgttaaactg aacctgcaaa
     2581 tgatcaaaca ttagatacac gaaagcccca gagaggtcgg attctctggg gctttttttc
     2641 aggttgatgt cgcctttatt gattttactt cttgtttcgg ttattcaacc aataactgaa
     2701 taaagagaca caaacttcta caagaagggg gcaaatgaac cgttcaaaca ttagatacac
     2761 ctccttccag tcgcaagact gtcggatagg cgacaagtta ttatacctta caaaataaaa
     2821 tctgtgtggt gtttttaagt atcccttatt ttcgggttgt tagggcttgc cctgaccgcc
     2881 tgtaaggcgc tttaagaaca agataaggtt ttttacccaa gactgtctta aaacgactta
     2941 gacgagctat cagaggcttc ccccacctcc gttactgact tgtttttagc aaaggagcct
     3001 ttaggcgact gcgcagtaca atgtgagagc atagcgatca cactttttta gcgaccgtag
     3061 ggagcaaggt tttgaattgg cagttttgaa aaatgcgcga acctgttggt agaaaaaaga
     3121 agtggttttt aggcgggaaa ttttgtcacc ctcttttata atctcttttt aaaccttttt
     3181 ttaaacctct ttaccgtcgg ctgtgtcctt actccttcaa tggatacagc gttttataaa
     3241 ctacattttg tctgctttta aactacattt tgtctgcttt taaactacat tttgtctgct
     3301 tttaaactac attttgtctg cttttaaact ctttttttgc actcacgtat gttataatat
     3361 gcattatcac agacaaaacg ttgtttataa cgtctgtatc cattgaagga gtaaggagga
     3421 atatctttgg caaatgaaat cgtgaaatac catcatgaat taaataccat tccgttgcgg
     3481 aaatttacac cagttgaaat gaatctattt ttctctattg tgtctagaat gcgcgaaaaa
     3541 ggcgataaaa cagtccgtct tacttttgac caactaaaag atttaagtaa ttataaagca
     3601 actgctaata atcgtttcat taatgattta gaaatgactt atgataaatt aatggattta
     3661 cgttttggta aaagaagtgc taatgggctc gatatagata gattcgttat gtttagtgag
     3721 tttaaaatac gtggaactgc taaagatcca tatgtagaca tacaagttta cgaaaaagct
     3781 ttacctttac taaacaattt agatgagtgg gttcgctatt cactccaaca atttaacgaa
     3841 ttacaaagta gctatgcaaa gactatgttt cgattactaa aacaatatcg aactaaaggt
     3901 tttgcttatt tttctaaaga ggattttcat gaactattag atattccaaa aagttactgg
     3961 aacaaccaag caaacgttga taatcgtgtt ataaagccta tacgacagga attaaccgca
     4021 atcttcaaag gattatcaat caaaaagaaa tacggaaaag ggcgaggaaa acctgtcgtt
     4081 ggctatcaat ttacgtttaa accagaaatc aaaaaagcag atgattttaa taaacatgca
     4141 ccaaccaaag aagaacaaaa taaagcattg gctttagctg ataaacaaaa agaatacgaa
     4201 gaacctgcta caacaattaa cgaaaaaatc aaacagaaga aacgagaact tgaactcctc
     4261 caacaaatca aacaactaga aaaagagaaa gaagctcttg aaaaacaaac agagtccgca
     4321 ctttcgaaaa aagagaaaaa aagtttatta tctgatttag ataatttatt ccagtagcaa
     4381 cctaggaggc aaccagtatg caaccacaag aaacctttac aataaaggaa ttgtcagaac
     4441 tttttaagat gagtaggcaa gcaatatcga aacatatcca aaaattagat tcttccatga
     4501 tagctaaaaa tgagcgtggt tataaggttg tattgcgttc tggagtattg caacttgctc
     4561 gcaacttagg caaccaaatg ttgctagata ggttgcacga gtacgaacaa gacaacgata
     4621 aaaatgttaa tataccaagc aaaaacgtgg ttacaaacaa gttgctagat cagttgcgtg
     4681 aagaaaatga atttttacga gaacaacttg ccctcagaga gcaaaataat tctcatatac
     4741 aaaaactact cgaccagcaa caacaactca ccctacaagc caacaaacaa atcgaaaaac
     4801 tgcaagaaaa actacagttg acttatacag aagaagctcc agagaatgac tcaacgactc
     4861 tttctgaagc aacagaaaca atagacaaac aaattgatac tgaaaaaaaa tggtggcaac
     4921 tttggaaata actggaggga acattcatga caagaaaaaa tcttctattc ggttctattt
     4981 tactaggaat tctaggattc agtccgatgt tattgtcagt gatcaataaa gggaataaac
     5041 atggtgaatt gaaattgaat ccaaattatt acaacagtcc taacgaagaa ttgtttgtaa
     5101 aagaaacaga taaataataa aaaagactgc tctttcgagc agccagttaa aaaataatga
     5161 gtgtttattc ttgaaaaagc aacgcgctta tgaagcgcta ccaaaaattt atcatatttt
     5221 attaaaaata acaacccagt aaaaaatgac tggtaaaaat tcacaaagaa acggaaagaa
     5281 gcaaccgcag aaaatcaagc ttaaaaagag cttttgacct agaccaatcg aaaatcacaa
     5341 agtgagcgcc tgtatctatt gctacaggcg ctacttggca tagtgtacat tatgtcaaca
     5401 agaatttaat ataacatatt ttcctaatgc attctaacta taccacatta tcaaaaatat
     5461 aaattatctt tactaaataa aatattgaca agcctatacc ttttgtttta tgattttcac
     5521 aaagaaaacg ttttctatat atttttctgt atcaggagga aattctatga aaaaatttac
     5581 tttaggggta gctttgttaa ctattggggc agttgtttta ccgtcaacta ctatagttta
     5641 tgcttcagaa aattcccaag tggtatcatt aaacgaaaac aatgctactg aaaatgtagt
     5701 tattgagtac actgaagatg gtatggtcgt aactaaatat gaggatggtt atagtgcgga
     5761 agattttgca aattcttcag atatcatgac aagagctgca gttgcaagat ggggcaaaaa
     5821 ggaatataca aatattgctg taaccactgg agtagctgct aatgctatta atgctgcctt
     5881 ttgggcagga gggaaagcta gcacttctat gtttggtatc ccaggttggg caattggtgc
     5941 tttgttaaca ggtgcaagtt ggactaaaaa aggtgcttct ccagggaatg cagttgcaaa
     6001 agaatgggac aaaaatggta atggctggat tggattttat taccaaaggg gatatgatgc
     6061 agctggaaga gttgtagcta ctagatattc aacgttataa gttaaggagt gatgtttttg
     6121 atatctaaat cattaaaatc attaaaaaca tttactaaaa agaatccgca aatagctact
     6181 attcgtattt ttatatttgc tatagctgtt gctataattg ttaatatgct aagcggaggg
     6241 caattataca aatctggttt atttttagtg ttaatcttag ttgtcttaga cataataaag
     6301 tct
//
LOCUS       DARBET010000108         6285 bp    DNA     linear   BCT 07-NOV-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4027
            SAMEA114569841-rid20112183.denovo.112, whole genome shotgun
            sequence.
ACCESSION   DARBET010000108 DARBET010000000
VERSION     DARBET010000108.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA114569841
            Sequence Read Archive: ERR12203666
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 6285)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6285)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001972618.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001972618.1
            Genome Coverage       :: 132x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/07/2023 13:18:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,041
            CDSs (total)                      :: 2,975
            Genes (coding)                    :: 2,908
            CDSs (with protein)               :: 2,908
            Genes (RNA)                       :: 66
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 67
            CDSs (without protein)            :: 67
            Pseudo Genes (ambiguous residues) :: 0 of 67
            Pseudo Genes (frameshifted)       :: 26 of 67
            Pseudo Genes (incomplete)         :: 28 of 67
            Pseudo Genes (internal stop)      :: 20 of 67
            Pseudo Genes (multiple problems)  :: 6 of 67
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6285
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA114569841-rid20112183.denovo.112"
                     /isolate="VRE4027"
                     /db_xref="taxon:1352"
                     /country="Denmark"
     gene            <1..177
                     /locus_tag="SCJ86_002625"
     CDS             <1..177
                     /locus_tag="SCJ86_002625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_464634.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEG1170113.1"
                     /translation="QSLVLLAIVVIGLFLGFKREFSKLIGFLVIALIAVGLVFNASGV
                     KDVLLNLFNRIVGA"
     gene            190..690
                     /locus_tag="SCJ86_002626"
     CDS             190..690
                     /locus_tag="SCJ86_002626"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288934.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antirestriction protein ArdA"
                     /protein_id="HEG1170114.1"
                     /translation="MEQIRVYIANLGKYNEGELVGAWFTPPVDFDEVKERIGLNDEYE
                     EYAIHDYELPFEIDEYTPIEEINRLCGLAEELKGTPIGEVASEIQHAFFNSFEEMVEH
                     VDDIIYYPDCNDMSDVAYYLIVEAGDLGEVPEHLKTYIDFDACGRDLEIGGNYLVTSR
                     GVFELM"
     gene            787..2634
                     /locus_tag="SCJ86_002627"
     CDS             787..2634
                     /locus_tag="SCJ86_002627"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288935.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PHP domain-containing protein"
                     /protein_id="HEG1170115.1"
                     /translation="MVGNKWYKCDLHIHSTASQCFQDKKVTAKEWVEECLNKGLDCVA
                     LTDHNTGENIDEYKKVAIENGLDFFPGVELTCGENGTHLLVLFDKSACTNDISDFLTK
                     MGIKREDWASAAACSMKSVLEVIDFAEHEGLLVIPAHIDEFNGISSAKFGNLGKIFES
                     ENIYAVQTVQKEFFENRSQMIPSSKRDTIYDSVNARYDGRVGKDTLESWYKSVVEAEK
                     NNMTFLSFSDNPHSKGNSKHGLWGIGTRYSYIKMKDEPDLGSLRDALMLGETRVHSDF
                     SNFSINENEVLLEKLSFSGTTLSTKEVVVEFSDNLTSIIGGRGTGKSCITRFLVYVLG
                     KEAELDQFSEIQSDYQNFAQIEHNGSGIFLKDTIVKLNIFYKGTKYQIIRTQDRHSIY
                     EFTQENGLVEKETERLRMISDKVSIYSQKQIYEISKNQTSILELLDGYNSDLISEYKN
                     EIETCVNEIRKLNWDIVSVKKEIQDKAKVELEIEDLKKQVEKLSHKSYKDVYDEYSKE
                     TDIYRELKRDVEALKEIPKNLTDSVDKIDFGNGIKVTDEIDEHRGELIKNLVTNRAKI
                     QTIINEMSDEIDSYRAELNKSDWKVNYNEVSKRYQRGIKNLVKSYLKMN"
     gene            2637..3533
                     /locus_tag="SCJ86_002628"
     CDS             2637..3533
                     /locus_tag="SCJ86_002628"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305705.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HEG1170116.1"
                     /translation="MLQNLDSLYQSLSKTEERLEEIIQQEIKLKDYQVEVDSAMSRMN
                     KSYQNLYSERSSSSREIFSDITGIEATIQPNRDFNNYVKELRACINKEDTFDSEFGSL
                     IDKLTKNSITNQDIYNDILDIRVSGESSIFSDKKLINSIRKLNDAQVLELQTLKPNDL
                     VVIRLELNGKKVSLSNASAGQKTSAILSMILAYGNSPLILDQPEDDLDSQLINNLIVR
                     SIITKKENRQILIVTHNANIPVNGDSEWLISMGDSKEISADQCGSVDEPNIKPRICTV
                     MEGGEDAFTNRAKRYGFKQIVE"
     gene            3583..3972
                     /locus_tag="SCJ86_002629"
     CDS             3583..3972
                     /locus_tag="SCJ86_002629"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335930.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein"
                     /protein_id="HEG1170117.1"
                     /translation="MKKIKSYTSIWAVEKVIYAINDFQLPFPVTFNQMAWFVLSLLFV
                     IVFAHVPPLSMIEEVFLKYLGIPVAVTWFMSQKTFDGKKPLGFLRSFISYHLRFKVTF
                     AGKKVKEQKKRWDEPSTLVRSVNYVSD"
     gene            3959..>6285
                     /locus_tag="SCJ86_002630"
     CDS             3959..>6285
                     /locus_tag="SCJ86_002630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335929.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HEG1170118.1"
                     /translation="MYPIKYIENNLVFNQEGECFAYYELVPYNYSFLSPEQKFQVHDN
                     FRQLIAQNREGKIHALQIATESSIRATQERSKKEITGRLKEVAKQRIDIQTDALVSMI
                     GDSQIDYRFFIGFKLIATDEEVNLKSLKKSFFSGLQEFVYGVNHHLMGDFVSLSNEEI
                     RCYSKLEKLMESKLARRFKVRRVTPSDLTYLIEHIYGEKGIPFEEYEFQLPKKKLKSE
                     TLVKRYDLLRPNRCLIEEKPRCLRMEHENHESYVAYLTINTIVGEMEFPSSELFYYQQ
                     QQFTFPIDTSMNVEIVTNKKALATVRNKKKELKDLDNHAYQSDNETNSNVLDALDSVD
                     ELETTLDQSKESMYKLSYVVRVSAESVDELKRRCDEVLDFYDDTNVKLVRPFGDMMGL
                     HEEFLPSSKRYMNDYIQYVTSDFLAGLGFGATQMLGELEGIYFGYNVDTGRNVYLKPA
                     LASQGVKGSVTNALAAAFLGSLGGGKSFSNNLLVYYAVLFGGQAVIVDPKGERGGWKE
                     TLPEIADEISILNLTSKSENQGLLDPYVIMKKKKDSESLAIDILTFLTGISSRDGEKF
                     PVLRKAIRTVTQSDQRGLLQVINELRKDGSPIAENIADHIESFTDYDFAHLLFSDGSI
                     TQSISLDKQLNIIQVADLVLPDAETTFEEYTTMELLSVSMLIVISTFALDFIHSNREI
                     FKIVDLDEAWSFLQVAQGKTLSNKLVRAGRAMNAGVYFVTQNSDDLLDERLKNNIGLK
                     FAFRSTDIHEIKKTLEFFGLDQEDQSNQKRLRDLE"
BASE COUNT         2118 a          932 c         1347 g         1888 t
ORIGIN      
        1 caatctttag tcttactagc gattgtggtc attggtttgt ttttaggatt taaacgcgag
       61 ttttccaaac tgattggctt cttggtgatt gctttgattg cggtaggtct ggtctttaat
      121 gcttctggtg tgaaggatgt cttactcaat ttgtttaacc gaattgtcgg tgcctgaggg
      181 ggaatcacaa tggaacagat acgtgtttat attgcgaacc ttggaaaata caatgaaggt
      241 gagttggtgg gggcatggtt taccccaccc gtcgattttg atgaagtgaa agaacggatt
      301 ggattaaatg acgaatatga agagtacgca attcatgatt atgaactgcc ttttgagatt
      361 gatgagtata caccaattga ggaaattaat cggttgtgtg gtttagcgga agagcttaaa
      421 ggaacgccaa ttggtgaggt cgcttcagag attcaacatg ccttcttcaa ttctttcgag
      481 gaaatggtag agcatgtgga tgatatcatc tattatcccg attgtaatga catgagtgat
      541 gtagcgtatt acttgattgt agaagctggt gatttaggcg aagtaccaga acacttgaag
      601 acgtatattg attttgatgc gtgtggtcga gacttggaga ttggcgggaa ttatttggtt
      661 acgagtcgag gggtttttga actaatgtaa atcatttgtt agaattgacc agaaatttct
      721 atcgtacttt tacttttcta tttgatatac tgaaatagaa aatatttgtt gataggggtg
      781 ttactaatgg ttggcaataa gtggtacaaa tgtgatcttc atattcattc aactgcgagt
      841 cagtgctttc aggataaaaa ggttactgct aaggaatggg tagaagaatg tttaaacaaa
      901 ggactcgatt gtgttgcatt aactgatcac aatacagggg aaaatattga tgaatataag
      961 aaagtggcta tcgaaaatgg cttagatttt tttcctggag tggaattaac gtgtggagaa
     1021 aatggaactc atttactagt cctatttgat aaaagtgcat gtacaaatga tatctctgat
     1081 ttcttaacca agatgggtat aaaaagagag gattgggcca gcgctgcagc ttgctcaatg
     1141 aaatcagttt tagaagttat tgattttgct gagcacgagg gcttgctcgt tattcctgca
     1201 catatagatg agtttaatgg aatttcttca gcaaaatttg gaaatctagg aaagatattt
     1261 gaatcagaaa atatatatgc agtacagacg gttcaaaaag aattttttga aaatcgctca
     1321 caaatgattc cctcatctaa gcgtgacact atctatgata gtgtgaatgc cagatatgat
     1381 ggtagagttg gaaaagatac tttggaatct tggtataaat cagttgttga ggcagaaaaa
     1441 aataatatga catttcttag cttttctgat aacccacact cgaaaggaaa ttcaaaacat
     1501 ggactttggg gaataggcac aagatattcc tatataaaaa tgaaggacga acctgacttg
     1561 ggatcacttc gcgatgcttt aatgctaggc gaaactcgag ttcattctga tttttctaat
     1621 ttctccatta atgaaaatga agttttattg gaaaaattaa gcttttctgg gaccactctt
     1681 agcacaaaag aagttgtagt cgaattcagc gataatttaa cttcaattat tggaggaaga
     1741 ggaacaggca aatcttgtat tacaagattc ttggtctatg tccttggaaa ggaagcagaa
     1801 cttgatcaat tttctgaaat acaaagtgat tatcagaact ttgcacaaat tgaacataat
     1861 ggctctggaa tatttttgaa ggatacaatt gtgaaactga atattttcta taaggggaca
     1921 aaatatcaaa taataagaac acaggataga cattctattt atgaatttac acaagagaat
     1981 ggtctggttg aaaaagaaac agaacggttg agaatgattt ccgataaggt gagtatatac
     2041 tctcaaaaac aaatttatga aatttcaaaa aatcaaacta gtatactaga attactagat
     2101 ggatataact ctgatttgat ttcagagtat aaaaatgaga ttgaaacatg tgtaaacgaa
     2161 ataagaaaat taaattggga tattgtttct gtgaaaaaag aaatacaaga taaggcgaag
     2221 gtagagcttg aaattgaaga tttaaaaaaa caagtagaaa agctatctca taagagttat
     2281 aaagatgttt atgatgagta ttcaaaagag acagatattt atagggaact gaaacgtgat
     2341 gtggaggcac ttaaggaaat tcccaaaaat ctaactgata gtgttgacaa aattgacttt
     2401 ggcaatggta taaaagttac agacgaaatt gatgaacacc gcggagaatt gattaagaat
     2461 ttagtgacca atcgggctaa gattcaaaca ataattaatg aaatgtcaga tgaaattgat
     2521 agttatagag cagagctgaa taaatcagat tggaaagtta actataatga ggtttcaaag
     2581 aggtatcaaa gaggtatcaa aaacttagtg aagagttatc tcaagatgaa ttaaaaatgt
     2641 tacaaaacct tgattcttta tatcaatctt tatcaaaaac tgaagagcga ttggaagaaa
     2701 ttattcaaca agaaataaag cttaaagatt accaagttga ggttgactca gcaatgtcaa
     2761 gaatgaataa atcatatcaa aatctctatt ctgagagaag tagttcctcg cgcgaaattt
     2821 tttctgatat tacaggaata gaggctacta ttcaaccaaa tagggatttc aataattatg
     2881 ttaaagagct cagagcttgt attaataagg aggacacttt tgacagtgag tttggatctc
     2941 ttattgataa gctgacaaaa aattccatta ctaatcaaga tatatataat gacatacttg
     3001 atataagagt tagtggggaa agtagtattt tttcagataa aaaattaatc aattctataa
     3061 gaaaattgaa tgatgcacaa gttcttgaat tacaaaccct taagccaaat gatttagtag
     3121 ttatccgact tgaactaaat ggaaaaaaag ttagtttatc gaatgctagt gctggtcaaa
     3181 agacatctgc aattctttct atgattttgg cgtatggaaa ttcaccactc atattggatc
     3241 agcctgaaga tgatttggat agtcaattaa ttaataactt aatcgtaaga agtatcatta
     3301 caaaaaagga aaacagacaa attttaattg taactcataa tgcaaatatc ccagttaatg
     3361 gtgattcaga atggttgatt agcatgggag attcaaaaga aatatcggct gatcagtgcg
     3421 ggtcggtaga tgaacccaat atcaagccaa gaatatgtac tgtgatggaa ggtggcgagg
     3481 atgcttttac aaatagagcg aaacgctatg gttttaaaca aattgtagaa taacactaga
     3541 gggtgagaca tattattatg tccgcccttt ttaggaggaa aaatgaaaaa aataaaaagc
     3601 tataccagta tctgggcagt tgaaaaagtc atttacgcaa tcaatgattt tcagttgcct
     3661 tttcctgtga cttttaacca aatggcttgg ttcgttttat ctttactgtt tgtcatcgta
     3721 tttgcccatg tgcctccact atctatgata gaggaggtct ttttaaaata cttaggcata
     3781 ccggtggctg tcacttggtt tatgtcgcaa aagacgtttg atggcaagaa accactggga
     3841 tttttacgat cctttatcag ctatcacttg cgatttaagg tgacttttgc aggaaagaaa
     3901 gtcaaagaac agaagaaacg atgggatgaa cccagtactc ttgtaaggag tgtgaactat
     3961 gtatccgatt aagtatattg aaaataatct cgtctttaac caagaaggcg agtgttttgc
     4021 ctactatgaa ttagtgcctt acaattactc ctttttgtca cccgaacaga aatttcaagt
     4081 gcatgataac tttcgccaac taattgcgca aaatcgtgaa ggcaagatcc atgcgttaca
     4141 aattgccaca gaaagtagta ttcgcgccac acaagaacga tcaaaaaaag aaattaccgg
     4201 tagactcaaa gaagtagcga aacaacgaat tgatatacag acagatgcat tagtatccat
     4261 gattggcgat agtcagattg attatcgctt ttttattggc tttaaactaa ttgcgacaga
     4321 tgaagaagtc aatctaaaga gtctcaagaa atctttcttt tctgggcttc aagaatttgt
     4381 ctatggagtg aatcatcatt tgatgggcga ttttgtttcc ttatccaatg aagaaattcg
     4441 ctgctattct aagcttgaaa aattaatgga aagcaaattg gctcgaagat ttaaagtcag
     4501 gcgagtcaca ccgagtgacc tgacgtattt gattgaacat atttatggcg aaaaagggat
     4561 accgtttgaa gaatacgaat ttcagctacc aaagaaaaag ctaaaatcag aaacactggt
     4621 gaaacgttat gacttattac gtccaaatcg ttgtttaatt gaagaaaaac caaggtgctt
     4681 acggatggaa catgagaatc atgagtcgta cgtagcgtat ttaacaatta atacaattgt
     4741 gggggagatg gaatttcctt cctcagaact tttctattac cagcaacaac aatttacatt
     4801 cccaatcgac acaagtatga atgtagagat tgtgacgaat aaaaaggctt tagctaccgt
     4861 tcgtaataag aaaaaagaac tgaaagattt agacaatcat gcttaccaat ctgacaatga
     4921 aacgaattcc aatgtcttag atgctttgga ttccgtggat gaattggaaa ccaccttgga
     4981 tcagtcaaaa gaatctatgt acaaactgag ttatgtggtt cgagtgagtg ccgaatcagt
     5041 ggatgaacta aagcgacgct gtgatgaagt gctcgatttt tatgatgata ccaatgtaaa
     5101 actagtccgg ccctttggcg atatgatggg gctacatgaa gaattcttac cctcaagtaa
     5161 acgatatatg aacgactata ttcagtatgt tacgtctgat tttttggcgg ggcttggttt
     5221 tggggctact cagatgctag gagaattaga agggatttac tttggctaca atgtggatac
     5281 aggtcgtaat gtttacctga aaccggcatt ggcttcacaa ggagtcaagg gttcagtaac
     5341 caatgcgctg gccgccgctt tcctgggttc tcttggtgga ggtaaatcct ttagtaacaa
     5401 tctcttagtc tactatgctg ttttgttcgg tggtcaagct gtgattgtcg atccaaaagg
     5461 ggagcgagga gggtggaaag aaaccttgcc agaaattgcg gatgaaatca gtatcctcaa
     5521 cttgacgagt aagtcagaaa atcaaggtct gcttgatcct tatgtgatta tgaagaaaaa
     5581 gaaagactcg gaaagtttag caattgatat tctgactttt ttaacaggaa tttcaagtcg
     5641 agacggggag aagtttcctg tgttacgtaa agccattcga acggtgactc aaagtgatca
     5701 acgggggtta ttacaagtta ttaacgaatt gcgaaaagac gggtcaccaa tcgcggaaaa
     5761 tatcgccgat catatcgagt cctttaccga ttatgatttt gctcatttgc ttttctcaga
     5821 cggctcgatt acccaatcga ttagtttaga taaacagcta aatatcattc aagtggcgga
     5881 tttggtttta ccggatgcag aaacgacttt tgaagaatat accaccatgg aattgcttag
     5941 tgtttcgatg ctaatcgtca tttccacttt tgccttggac tttatccata gtaaccgaga
     6001 aatcttcaaa attgtcgatt tggatgaggc ttggagtttt cttcaagtgg ctcaaggaaa
     6061 aacgttatcg aataagcttg ttcgtgctgg tcgggccatg aatgctgggg tctatttcgt
     6121 cactcaaaat agcgacgatt tattagatga acgtttaaaa aataatatcg ggttgaagtt
     6181 tgcttttcgc agtaccgata ttcatgaaat caagaaaacc ttggaattct ttggtttgga
     6241 tcaggaggat caaagcaatc aaaaacgcct gcgagatttg gaaaa
//
LOCUS       DARBET010000109         6283 bp    DNA     linear   BCT 07-NOV-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4027
            SAMEA114569841-rid20112183.denovo.113, whole genome shotgun
            sequence.
ACCESSION   DARBET010000109 DARBET010000000
VERSION     DARBET010000109.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA114569841
            Sequence Read Archive: ERR12203666
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 6283)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6283)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001972618.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001972618.1
            Genome Coverage       :: 132x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/07/2023 13:18:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,041
            CDSs (total)                      :: 2,975
            Genes (coding)                    :: 2,908
            CDSs (with protein)               :: 2,908
            Genes (RNA)                       :: 66
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 67
            CDSs (without protein)            :: 67
            Pseudo Genes (ambiguous residues) :: 0 of 67
            Pseudo Genes (frameshifted)       :: 26 of 67
            Pseudo Genes (incomplete)         :: 28 of 67
            Pseudo Genes (internal stop)      :: 20 of 67
            Pseudo Genes (multiple problems)  :: 6 of 67
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6283
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA114569841-rid20112183.denovo.113"
                     /isolate="VRE4027"
                     /db_xref="taxon:1352"
                     /country="Denmark"
     gene            59..1066
                     /locus_tag="SCJ86_002631"
     CDS             59..1066
                     /locus_tag="SCJ86_002631"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347627.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide biosynthesis protein"
                     /protein_id="HEG1170119.1"
                     /translation="MFKGKTILVTGGTGSIGSEIVRQLLKYEPKAIRIYSRGEEKQFY
                     MQQELSNHTNLRFLIGDIRDSQRIDYACRDVDIIFHAAAMKHVPASEYNPMEAVKTNV
                     MGTQNVIDAAIKNNVKHFVGISTDKVVSPTNTMGATKLLSEKLIIAANQYKGKTETIF
                     SCVRFGNVMGSSGSVIPLFSKQIKNNKDITLTDVDMTRFMMSIPDAARLVLSAAMNSK
                     FGETYVLKMPAINILTLAQSLIKIYNDTHDEKYRGEIKVVGIRPGEKLYEELMTTDEI
                     ERSYENDKMYIIPPHYHATDELYKDYEKIKITAYSSDATELMSGQEFIRIAKENNVVT
                     W"
     gene            1078..2082
                     /gene="neuB"
                     /locus_tag="SCJ86_002632"
     CDS             1078..2082
                     /gene="neuB"
                     /locus_tag="SCJ86_002632"
                     /EC_number="2.5.1.56"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292874.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylneuraminate synthase"
                     /protein_id="HEG1170120.1"
                     /translation="MKSEVYVIAEIGVNHNGSLDLALESIDKAKECGANAVKFQTFKT
                     NKLVSKKAPMAKYQKDNLKEDSSQFEMLKKLELSQSDFIRIKDYCIQNKIDFLSTPFD
                     EESADFLKQIGVQGFKIGSGDLTNLPFLRKIDMYGLPVLLSTGMADIHEVSEAVDCFI
                     DSPVTILHCTSNYPASSEDINLLAINTLKEMFGIPVGYSDHSLGYDVGICAVAMGAKV
                     IEKHFTLDKELPGPDHKASLDPQEFADFVKHIRNTEIFLGDGVKRAMPSELSTKEVSR
                     KSLVVTENLNVGDILTEKSLTIKRPGNGIAPKFLYQYIGKKVKRPISADSVLLEEDVE
                     "
     gene            2079..3227
                     /gene="neuC"
                     /locus_tag="SCJ86_002633"
     CDS             2079..3227
                     /gene="neuC"
                     /locus_tag="SCJ86_002633"
                     /EC_number="3.2.1.183"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177093.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylglucosamine 2-epimerase
                     (hydrolyzing)"
                     /protein_id="HEG1170121.1"
                     /translation="MKKILIVSGTRADYGIYKPVAENLLQDNWDVSFVVSGMHLSHKY
                     GFTKELILADGFRISGEVQSLFLENNKGNMARSVALEINGFTNIFEQTSPDFVLLLGD
                     RGEMLAAAIACLYLGIKTAHIHGGEVSGTVDESIRHAITKIASVHFAATEKSKERLIK
                     MGEDSWRVFPVGAPRIDTILNNRLPSFRDIKEKYHLKFEKKKYVLQVFHPVVTELENI
                     EKQILALINSMEQQSQPIICILPNSDAGSEIIRKMYQKYLKTDIIYITNLEPEEYLTI
                     LKECKFMIGNSSSGIIEAASFKIPVINLGTRQNGREQSKNIINANLNENEIETALNKI
                     QDPQFIKSLKNVKNIYGDGNSSRRITKTLNQILNDGEDIKWIQKQIAY"
     gene            3200..3877
                     /locus_tag="SCJ86_002634"
     CDS             3200..3877
                     /locus_tag="SCJ86_002634"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177094.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyltransferase"
                     /protein_id="HEG1170122.1"
                     /translation="MDSKTNSLLKYTSDPYIIIGAGGHAKVIIETLRLENKLIYGFFD
                     DKQNKMQDLKYLGKINELNSWADSKKKIYKFIVAIGDCVVRKKIIKKLNMPSEQYGIA
                     VHPSAIVAKEISIGYGTVIFANAVINPDAVIGEHAIINTGSIVEHDCRINNYVHLSPG
                     VCLAGGVHVGVGTQIGIGSQCIQMKKIGSWCMIGAGSTIVKDIPSHSLAYGNPAKIKK
                     EGINFEF"
     gene            3867..5009
                     /locus_tag="SCJ86_002635"
     CDS             3867..5009
                     /locus_tag="SCJ86_002635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326811.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class I/II-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HEG1170123.1"
                     /translation="MNSRILLSSPHMGGTEEKYVAKAFKDNWIAPLGENVDKFEESIT
                     KYTGSKGGASALSSGTAAIHLALRLLNVGKNDIVFAPSFTFIATVNPILYQGAVPVFI
                     DSEPNTWNMSPVALESALEEAERNNQLPKAIIVVNLYGQMADYERILAIANQFNVPVI
                     EDAAESLGSTYKGISSGLFGTIGIYSFNGNKIITTSGGGALVSSDKKYVEHSRFLATQ
                     ARDQAPYYQHSEIGYNYRLSNISAGIGRGQMEVLNDRVAKRREIFNRYKEAFSGIDRI
                     EMMPEVDGMVPNRWLSTLTINDYNSIDQVVNVINKLNEKNIETRALWKPLHLQPVFQG
                     VKMYYHDNNLSVSEKLFNSGLCLPSGSNMTDSEQEFVIDEFKKIFK"
     gene            5022..5999
                     /locus_tag="SCJ86_002636"
     CDS             5022..5999
                     /locus_tag="SCJ86_002636"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342242.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HEG1170124.1"
                     /translation="MNIGIIGLGSMGKRRLRLIQEHFNDILLCGVDTNEARRKEVEKE
                     FGIPTYNSLTEAKNLFEINSVFVCTSPITHEFIIKEALNLDCNIFTEINLLNEYYDEI
                     IEVAKKKDRLLYLSSTFLKRKEIQYIKSKVTHNKKISYQYHVGQYLPDWHPWEDYRNF
                     FVSNKKTNGCREIFAIELPWIIDTFGKVKSFTVMTQKLSNLDIDYSDSFTLIIEHETG
                     IVGTLSVDVVSRVAKRNLTIIGEKILIEWEGNPDSLFSWNEETSSMTDIQLYDEVMHD
                     NNYSHTIIEDAYLEEIKEFFTLLEKKMEPQYTFEKDKYIINLINDIEVN"
     gene            5999..>6283
                     /locus_tag="SCJ86_002637"
     CDS             5999..>6283
                     /locus_tag="SCJ86_002637"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177096.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oxidoreductase"
                     /protein_id="HEG1170125.1"
                     /translation="MMKVIFFGLGSIGKRHLKNLISYGKKNSINFDITAYSSSGKKEF
                     EEITYIQNRDDLDTHYDIAFITNPTFKHLETLNVIVEKVDYIFLEKPAFSE"
BASE COUNT         2346 a          790 c         1206 g         1941 t
ORIGIN      
        1 ttttttaaag attaagaatc gaattataga tgactataat tcataggagg agatataagt
       61 gtttaaagga aaaacaatat tagtaactgg cggaactggt tccattggaa gtgaaattgt
      121 tcgtcagctt ttaaaatatg aaccaaaagc tatcagaatt tatagtagag gtgaagaaaa
      181 acaattttat atgcaacaag agctctcaaa tcacactaat ttgagatttt taattggtga
      241 tatcagagac tctcaaagga ttgattatgc atgtcgtgat gtggatatta tttttcatgc
      301 tgccgcaatg aaacacgttc cagcaagtga atataatcct atggaagctg taaaaacaaa
      361 tgtgatggga actcaaaatg taatagatgc agcgatcaaa aataatgtaa aacattttgt
      421 aggtatcagt acagataaag tagtgagtcc tactaacacc atgggtgcga caaagttatt
      481 gagtgaaaaa ttaataatag ctgccaatca gtataaagga aaaacagaaa caatattttc
      541 ttgtgtaagg tttggaaatg tcatggggtc aagtggatct gttattcctc tttttagtaa
      601 acaaataaaa aacaataaag atattacttt gacagatgtt gatatgacac gatttatgat
      661 gtcaataccg gatgctgcaa gattagttct gtctgctgct atgaattcaa aatttggaga
      721 aacatatgtc ttaaaaatgc cggcaatcaa tattttaact ttagctcaat cattaataaa
      781 aatatataat gatacacatg atgaaaaata tagaggtgaa ataaaagttg taggtatcag
      841 gccaggagaa aaattatatg aagagctaat gactacagac gaaattgaac gctcatatga
      901 aaatgataaa atgtatatca ttccaccaca ctatcatgct actgacgaac tgtacaaaga
      961 ttatgaaaaa attaaaatca ctgcatactc ttcagatgca acagaattaa tgagtggaca
     1021 ggaatttatt agaatcgcaa aagagaataa tgtcgtgact tggtaaggag ttttatgatg
     1081 aaatcagaag tatatgtaat tgcagaaatt ggtgtaaacc ataatggaag tctagattta
     1141 gctttagaaa gtattgataa agctaaagaa tgtggagcta acgcggttaa atttcaaaca
     1201 tttaaaacga ataagctagt ttccaaaaag gctccaatgg caaaatatca gaaggataat
     1261 cttaaagagg acagttctca atttgaaatg ctaaaaaaat tagaattatc tcaatctgat
     1321 tttattagaa taaaagatta ttgtatacaa aataaaattg attttttgtc tactcctttt
     1381 gatgaagaaa gtgcagactt tctaaaacaa attggtgtac aaggatttaa aattggctca
     1441 ggagatttaa caaatttacc ttttcttaga aaaatagata tgtatgggtt gccagtttta
     1501 ttatctacag gtatggctga tattcatgag gtcagtgagg ctgtagattg tttcatagat
     1561 agtccagtta ctattttaca ttgtacttca aattatccgg ctagttctga agatattaat
     1621 ttattggcga ttaacacatt aaaagaaatg ttcgggatac ccgttgggta ctccgatcat
     1681 tcattgggat atgatgttgg tatttgtgct gttgcgatgg gtgctaaggt aattgagaaa
     1741 cattttacct tggataagga actacctggt ccagatcata aagcttcttt agatcctcag
     1801 gaatttgcag attttgtaaa gcatattaga aacactgaaa tatttttagg tgatggtgtt
     1861 aagcgagcaa tgccttctga acttagtaca aaagaagtat caagaaaaag cctagtggtc
     1921 actgagaatt taaatgtggg agatattctt acagagaaat ctcttacaat aaaacgtcct
     1981 ggaaatggaa tagctcctaa gtttttatat caatatattg gcaaaaaagt taaaagacca
     2041 ataagtgcag atagtgttct gcttgaggaa gatgttgaat gaaaaaaata cttatagtaa
     2101 gtggaacacg agctgattat ggaatatata aacctgttgc tgagaattta ctacaagata
     2161 attgggatgt atcatttgtt gtttctggaa tgcatttatc ccataaatat ggatttacta
     2221 aagaattgat cttagctgac ggatttagaa ttagtggaga agttcaaagt ttgtttttag
     2281 agaacaataa gggaaacatg gctcggtctg tggcattaga gataaatggt ttcacaaata
     2341 tatttgaaca aactagccct gattttgtat tattactagg ggatagagga gaaatgttgg
     2401 cagctgcgat agcttgtttg tatcttggaa tcaaaacagc acatattcac ggaggcgaag
     2461 tgtctggtac tgtagatgaa tcaataagac atgccataac gaaaatagca tctgttcatt
     2521 ttgctgctac ggaaaaaagc aaagagcgtt taataaaaat gggggaagat tcttggagag
     2581 tttttcccgt cggagctcct agaatagata ctattttaaa taatagatta cctagtttta
     2641 gagatataaa agaaaaatat catctaaaat ttgaaaagaa aaaatacgtt ttgcaagtat
     2701 ttcatccagt tgtaaccgaa ctagaaaata tagaaaaaca aattttagct ttgatcaatt
     2761 caatggaaca acaatctcaa ccaattattt gcattttacc taatagtgat gctggtagcg
     2821 aaattatcag aaaaatgtac cagaaatatt taaaaacgga tattatttat attacgaatt
     2881 tagagccgga ggaatacctg actatattaa aagagtgcaa atttatgatc ggaaattctt
     2941 cttctgggat aattgaagct gcatcattca aaataccagt aataaattta ggaaccagac
     3001 aaaatggacg agaacaatct aaaaatatta taaatgcaaa tctaaatgaa aatgaaattg
     3061 aaactgcttt gaataaaata caagatcctc agtttataaa aagcttgaaa aatgttaaga
     3121 acatctacgg tgacggcaac agttctagaa gaataacaaa aacattgaat caaatattga
     3181 atgatgggga agatattaag tggattcaaa aacaaatagc ttattaaaat atactagtga
     3241 tccatacatt attattgggg ctggcggaca cgcaaaagtc attattgaaa ccttaagatt
     3301 agaaaataag ttaatatatg gattttttga tgataagcaa aataaaatgc aagatctgaa
     3361 atatcttggg aaaataaacg aattgaatag ctgggcggat agcaaaaaaa aaatctataa
     3421 atttattgta gctattggtg actgtgtagt aagaaaaaag attataaaaa aattgaatat
     3481 gccaagtgag caatatggaa tagctgttca tccaagtgct atagtggcaa aagaaatttc
     3541 aattggttat ggaactgtta tttttgcgaa tgctgtaatt aacccagatg ctgtaattgg
     3601 agagcatgct ataataaaca caggtagcat tgttgagcat gattgtagaa ttaataacta
     3661 tgttcattta tctccaggtg tatgcttagc gggtggagta catgtaggtg taggaactca
     3721 aataggaatt ggttctcaat gtatccagat gaagaaaatt ggatcttggt gtatgatagg
     3781 cgcagggagc actattgtta aggatattcc ctcacattcc ttagcttatg gcaatccagc
     3841 taaaattaag aaagaaggaa ttaattttga attctagaat tttattatca agtcctcata
     3901 tgggaggaac agaagaaaaa tatgttgcta aggcttttaa agataattgg atagcgccat
     3961 taggtgaaaa tgtggataaa tttgaagaaa gtattacaaa atatactggt tctaagggcg
     4021 gagcttctgc gttaagttca ggtacagctg ccatacatct tgccttaaga ctgcttaatg
     4081 ttggaaaaaa cgatattgtc tttgctcctt catttacttt tatagctaca gtaaatccta
     4141 ttttatatca aggggctgta ccagtgttca ttgactcaga acctaataca tggaatatga
     4201 gccctgttgc actagagagc gcattagaag aagctgaaag aaacaaccaa ttacctaagg
     4261 caatcatcgt agtgaattta tatggtcaaa tggctgatta tgaacgaatt ttagctattg
     4321 caaaccaatt taatgttcca gtgatagaag atgcagcgga atcgttaggt tctacttata
     4381 agggaatttc gtcaggctta tttgggacaa ttgggatcta ttcatttaac ggtaataaga
     4441 tcatcacaac ctcaggtgga ggagctctag tgtctagtga taaaaaatat gttgagcatt
     4501 caagattttt agcaacacaa gcaagagatc aagcaccgta ttatcaacat agcgaaatag
     4561 gttataatta ccgtctaagc aatatttctg ccggtatcgg acgtggacaa atggaagtat
     4621 tgaatgatag agtagctaaa cgaagagaaa ttttcaatcg atataaggaa gctttttctg
     4681 gaattgatag aattgaaatg atgccagaag tggatggcat ggtgccaaat cgttggttgt
     4741 ctactttaac gatcaatgat tataattcta ttgatcaagt agttaacgta ataaataaac
     4801 taaatgaaaa aaacattgaa acaagagctt tgtggaaacc actacatttg caaccagtat
     4861 ttcaaggggt aaaaatgtat taccatgata ataatcttag tgtgtcagaa aagttattta
     4921 atagcggctt atgcttacct tctggaagta atatgacaga tagtgaacaa gaatttgtta
     4981 ttgatgagtt taaaaaaata ttcaagtagg tgtgatattt tatgaacata ggaattattg
     5041 gtttaggttc catggggaaa agaagactta gactcattca agaacacttt aatgatattt
     5101 tactatgtgg tgttgatact aatgaggcga gacgaaagga agttgaaaag gaatttggaa
     5161 taccaactta taatagtcta acagaggcaa aaaatttgtt tgaaataaat tcagtttttg
     5221 tttgtacatc ccctataacc catgaattta ttataaaaga ggctttaaat ttagattgta
     5281 atatctttac ggaaatcaat ttattaaatg aatattatga tgaaataatc gaagtagcta
     5341 aaaagaaaga tagattactt tatctatctt ctacattttt gaagagaaaa gaaattcaat
     5401 atattaagtc taaagtgacg cataataaaa aaatttctta tcaataccat gtaggacagt
     5461 atttacccga ctggcatcct tgggaagatt atcgcaattt ttttgtttca aacaaaaaaa
     5521 ctaatgggtg tagagaaata ttcgctattg aattaccttg gattatagat acatttggga
     5581 aagttaaatc gtttactgta atgacccaga aattatccaa tcttgatatt gattattcgg
     5641 acagtttcac tcttattatt gagcatgaaa ctggtatagt tggaacttta agtgtagatg
     5701 ttgtttcaag agtagctaaa agaaatttaa ctataattgg tgaaaagatt ttaatagaat
     5761 gggaaggtaa cccggattct ctattttcat ggaatgaaga aacttctagt atgacagata
     5821 ttcaattgta cgatgaggtt atgcatgata ataattattc acatactatt attgaagatg
     5881 catatttaga ggagattaaa gaatttttca cgcttttgga aaagaaaatg gaaccccaat
     5941 atacttttga aaaagataag tatattatta acttaattaa cgacattgag gtaaattaat
     6001 gatgaaagta attttttttg ggttagggtc aattggaaag agacatctaa aaaatctaat
     6061 aagctatgga aaaaagaatt ctattaattt tgatataaca gcttatagta gcagtggaaa
     6121 aaaagagttt gaagaaataa cgtatattca aaatagagac gatttggata cacattatga
     6181 tatagctttt attacaaatc cgacttttaa gcatctggaa acactaaatg taattgttga
     6241 aaaagttgat tacatatttt tagaaaaacc tgctttttct gag
//
LOCUS       DARBET010000110         6234 bp    DNA     linear   BCT 07-NOV-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4027
            SAMEA114569841-rid20112183.denovo.114, whole genome shotgun
            sequence.
ACCESSION   DARBET010000110 DARBET010000000
VERSION     DARBET010000110.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA114569841
            Sequence Read Archive: ERR12203666
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 6234)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6234)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001972618.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001972618.1
            Genome Coverage       :: 132x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/07/2023 13:18:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,041
            CDSs (total)                      :: 2,975
            Genes (coding)                    :: 2,908
            CDSs (with protein)               :: 2,908
            Genes (RNA)                       :: 66
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 67
            CDSs (without protein)            :: 67
            Pseudo Genes (ambiguous residues) :: 0 of 67
            Pseudo Genes (frameshifted)       :: 26 of 67
            Pseudo Genes (incomplete)         :: 28 of 67
            Pseudo Genes (internal stop)      :: 20 of 67
            Pseudo Genes (multiple problems)  :: 6 of 67
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6234
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA114569841-rid20112183.denovo.114"
                     /isolate="VRE4027"
                     /db_xref="taxon:1352"
                     /country="Denmark"
     gene            <1..1246
                     /locus_tag="SCJ86_002638"
     CDS             <1..1246
                     /locus_tag="SCJ86_002638"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297162.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="oligosaccharide flippase family protein"
                     /protein_id="HEG1170126.1"
                     /translation="LNIVDAVMRFLMDKDKNKKNIASIAIKVIFLSVIVFGSVMYLLN
                     ITKLWQDVYGLEKYIFLYYISYVWNQFLIQFAKGLEKVKAMGIAGIISTIVTIIANIF
                     FLLILKWGLIGFFIANILSQIISATYLSVLVRIWEYIDFEAKDPQLKREMLNYSIPLI
                     ATVVGWWINSTADRYVVSFIVGVSANGLLSVAYKIPQIINTLQGIFIQAWQISAIKEY
                     GDNETAKFYGNTFVMINLLMCITCSSLIILTRPFSHILYAKDFYEAWKYVPFLLIANV
                     LNCASGLLGPILSAKKDSKSMMWSAIIGALVNVIMSVVLVYKIGVQGATIATVICSYV
                     IYVVRKRAVGKDIFIKHYHIVVESWGLLFVQAIIEIYFKNYVIEIIIMIILIIINISY
                     IKQLCYRGRNIISMIKSREHKR"
     gene            1282..1998
                     /locus_tag="SCJ86_002639"
     CDS             1282..1998
                     /locus_tag="SCJ86_002639"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acylneuraminate cytidylyltransferase family
                     protein"
                     /protein_id="HEG1170127.1"
                     /translation="MVDNYERKNILKKKSIGCRMKIVAIVPMKLNNKRLPQKNTKAFT
                     NGKPLCYYILSTLLKVKQIDKVYVYCSNPDIKEFIPEGVTFLKRSSSLDQDTTSITQV
                     LAGFTKEVEADVYVMTHTTAPFISEKSIEKGLDAVLTGEYDSAFAAKKLQDFLWKDGE
                     PFNYELDNIPRTQDLPPLYEETSGFYIYNRNVMTELHRRIGKKPCIVEVGEIESVDID
                     EAEDFMIADAIYNHLILQGS"
     gene            2017..3600
                     /locus_tag="SCJ86_002640"
     CDS             2017..3600
                     /locus_tag="SCJ86_002640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldolase catalytic domain-containing protein"
                     /protein_id="HEG1170128.1"
                     /translation="MNNVQILDCTLRDGGYCNNWEFGFDNTKKIISGLIESGIEIIEC
                     GFLTNKTVYNKNVTKYTTLEELAQIIPEKREGKLFVAMMNYGEYNIEDLPDYDGSSID
                     GIRIAFHKKNCNEALKLCREIKKKGYLVFVQAMVSLNYTDEEFLAVIKNVNQFKPYAF
                     YIVDSFGMMKGKDLTRLFYMIEHNLEKDIQIGFHSHNNMQLAYSNAQKLITVQTNRSL
                     IIDSSVYGMGRGAGNLNTELFVEYLNDNAGKKYQLKPLLYIIDEILNSFYQKSYWGYS
                     LPNYISAVHNAHPNYASYLSYKNTLTIEAMNEIFEMMDEEKKVSYDKQYIEDLYYKYM
                     ETGKIQEEYMEELRKFLSDKKILLIAPGKSSVDEVERIKEFVTKEEVVIISVNFEYSH
                     IDIDYVFLSNLRRFHELPKESRTKCIVTSNIQASDVYLRTSYKKLLSEREVVRDNAGL
                     MAIRFLADMNVDEIFLAGFDGYSHNEDENYGEQSMEIITKFALLDAMNVQMRDELSEL
                     AKKVKVTFLTDPRWVRIDK"
     gene            3735..4313
                     /locus_tag="SCJ86_002641"
     CDS             3735..4313
                     /locus_tag="SCJ86_002641"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297159.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEG1170129.1"
                     /translation="MISILCIVAYHANLRLNYNADGIIVHGSLTANQIFLNYIGIWGQ
                     LGVNCFIFISAYFLSEKRNIHFENVLGIVLECIFYSVLVYGILCACGIETFSLNSFVK
                     EILSFALGRYWFITSYLALYILSPYLNIMLNNLEKLQLQKISMLLFIGVCCYRMLFKT
                     SLFGDCAVLIALYVICYTLKKTIIIFLRDTES"
     gene            4259..4777
                     /locus_tag="SCJ86_002642"
     CDS             4259..4777
                     /locus_tag="SCJ86_002642"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321992.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEG1170130.1"
                     /translation="MLYTEKNDNNIFERHRIIGTIVCITVIPLLISIGILFKFIPNAM
                     VNQMVHIGSPLMVIASIFLFYVFKNFSFYSKYINKLARTSLGVYILHENHYFIDGMKS
                     RGLIWDGIFGLGKRFLDRDFWIWFIIAVVVVCIICMAIDVVREKFERQIFKIKSVKNL
                     CKLMNHVINIYP"
     gene            4785..5825
                     /locus_tag="SCJ86_002643"
     CDS             4785..5825
                     /locus_tag="SCJ86_002643"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317228.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar kinase"
                     /protein_id="HEG1170131.1"
                     /translation="MRRKEVKVISFGELLLRLAAPGYSKLFQRDSLETTFCGGEANVA
                     VSLAIFGYQSEFITKLPDSDVGYAAANALRYFGVNVDNIIFGEGRMGLYYLEKGAAQR
                     PSKVIYDRKFSAISLADSDEFKWKEILNDANWFHWTGINPALSENLFETSLDACQAAK
                     KRGITISCDLNYRSKLWTSEEAKEKMEQILPLVDICICNEEDAEKVLGIKAEHTNINS
                     GELNKDGYVQVAREIHKKFGCRYVATTLRRSYSASRNGWSAMLYEAEKDKAYFSTEYN
                     IQIVDRVGGGDSFSAGIIYALCENMSGQKAIEFATASSCLKHTIEGDFNRTTIEDVYN
                     LMNSGGNGRVLR"
     gene            6074..>6234
                     /locus_tag="SCJ86_002644"
     CDS             6074..>6234
                     /locus_tag="SCJ86_002644"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS30 family transposase"
                     /protein_id="HEG1170132.1"
                     /translation="MTYTYLTTDELVIIESYLKINQSVAKTAHCLNRSSQTIHKVYLF
                     FKQRKSALE"
BASE COUNT         2261 a          708 c         1204 g         2061 t
ORIGIN      
        1 cttaaacatt gtagatgctg tgatgcgctt tttaatggat aaagataaaa ataaaaaaaa
       61 tatagcatct atagcgatta aagttatatt tttaagcgtg atagtctttg gatccgtaat
      121 gtatttgtta aatattacaa aattatggca agatgtttat ggattagaaa aatatatttt
      181 tttgtattat atatcctatg tatggaatca atttctaata caatttgcta aagggttaga
      241 aaaagttaaa gctatgggca tagcaggtat tatcagcaca attgtaacaa taattgccaa
      301 tattttcttc ttgcttattc tgaaatgggg attaattggt tttttcattg caaatatttt
      361 atcacagatt atttcagcta cttatctgtc agtattagtg agaatatggg aatatatcga
      421 ttttgaggct aaagatcctc agctaaagag agaaatgcta aattactcga ttccattaat
      481 tgcaactgtt gttggatggt ggattaatag tacagctgat aggtatgtgg tttcctttat
      541 agtaggtgtc tcggcgaatg gcttattgtc tgtggcatat aaaatacctc aaataattaa
      601 tactttgcag ggaatattta tacaggcctg gcaaatttct gcaattaaag aatatggaga
      661 taatgagact gcaaaatttt atggaaatac atttgttatg attaatttgt taatgtgcat
      721 aacctgttca agtcttatca tattaactag accgttttca catattcttt atgcgaaaga
      781 cttttatgaa gcgtggaaat atgttccatt tttattaata gctaatgttt taaattgtgc
      841 atcaggtctg ttaggaccta tcttatcagc aaaaaaagat tctaaatcca tgatgtggtc
      901 tgcaatcata ggggcattag ttaatgtgat tatgagtgta gtccttgttt ataagatagg
      961 ggttcaaggt gccacaatag ccactgttat atgttcatat gtaatatatg ttgttcgtaa
     1021 acgagctgtg ggaaaagata tttttataaa gcattatcat attgttgtgg aatcttgggg
     1081 attattgttt gttcaggcaa tcatagaaat atattttaaa aattatgtaa tagaaattat
     1141 aattatgatt attttaatta ttataaatat cagttatatt aagcagttgt gttatagagg
     1201 aagaaacatt atttcaatga ttaagagtcg agagcacaaa cggtagacga tgttattttt
     1261 ataaaaagat ataatttatt aatggtcgat aattatgaaa ggaaaaatat tcttaaaaaa
     1321 aagagtatag gatgtcgtat gaaaatagtc gctatagtac caatgaagtt aaataacaaa
     1381 agattgccac aaaaaaacac gaaagctttt accaatggaa aaccattgtg ttattatata
     1441 ctttcaacac ttttaaaagt aaaacagatt gataaagtgt atgtctattg tagtaaccca
     1501 gatattaaag aatttatacc agaaggtgta acatttttaa agagatctag ttccttagat
     1561 caggatacaa cgagtattac acaggtatta gccggtttca caaaagaagt agaggctgat
     1621 gtatatgtaa tgacacacac aacagcacca tttattagcg aaaaatctat tgaaaaagga
     1681 ctagatgccg tgctgacagg agaatatgat tctgcatttg ctgcaaaaaa attgcaagat
     1741 ttcttatgga aagacggtga accatttaat tatgaattgg acaatattcc tagaacacag
     1801 gatttgccgc ctttatacga agaaactagt ggattttata tttataatcg caatgtgatg
     1861 accgagttgc atagaagaat tgggaaaaaa ccttgcatag ttgaagtagg tgagattgag
     1921 tcagttgata ttgatgaagc cgaggatttt atgattgctg atgcgattta taatcatctt
     1981 attttacaag gtagttaaga cagggggaag gttaggatga acaatgtaca gattttagat
     2041 tgcacactta gagatggggg ctattgtaac aattgggaat ttggatttga taatacaaaa
     2101 aaaattattt ctggacttat agaatcagga attgagatta tagaatgtgg gtttctcaca
     2161 aataaaacag tatacaataa aaatgttaca aaatatacga ccttagaaga attagcacag
     2221 ataattcctg aaaaaagaga aggaaaatta tttgtagcaa tgatgaacta tggcgaatat
     2281 aatatagagg atcttccaga ttatgatggt tcatctattg atggaattag gattgcgttt
     2341 cataaaaaaa attgcaatga agcgttgaaa ttatgtcgtg agataaagaa aaaaggctac
     2401 ttagtttttg ttcaagctat ggtatctctc aattatacag atgaagagtt tttagcagta
     2461 ataaaaaatg tgaatcagtt taaaccatac gctttttata ttgttgatag ttttggaatg
     2521 atgaaaggaa aagatttaac tcgtcttttt tacatgattg agcataatct agaaaaagat
     2581 atacagatag gttttcactc acataataac atgcagcttg catattctaa tgcacaaaaa
     2641 cttataaccg tacaaacaaa tcgaagcctg attattgatt cttcggtgta cggaatggga
     2701 agaggagcag gtaatcttaa cacagaacta tttgtagaat atttgaatga taatgcagga
     2761 aaaaaatatc aattaaaacc attattatat ataattgatg aaatactaaa tagtttctat
     2821 cagaagagtt attggggata ttcattacca aactatattt cagcagttca taatgctcat
     2881 cctaattatg caagctatct gtcatataag aatactttaa caattgaagc aatgaatgaa
     2941 atattcgaaa tgatggatga agaaaaaaag gtatcttatg ataaacaata tatagaagat
     3001 ttatactaca aatatatgga aactgggaaa atacaggagg aatatatgga agaactgaga
     3061 aaatttctct cagacaaaaa aattctactg attgcaccag gaaaaagttc tgtagatgag
     3121 gtagaaagaa taaaagagtt tgttaccaaa gaagaagtcg taataattag tgtaaatttt
     3181 gaatattctc atatagatat agattacgta tttttaagta atttaagacg ttttcatgaa
     3241 ttaccgaaag aaagcagaac taaatgtatt gttacatcaa atatacaagc atcggatgta
     3301 tatttacgga cgagttataa aaagttgtta tctgaaaggg aagttgtaag agacaatgca
     3361 ggattaatgg caattagatt tttagccgat atgaatgtgg atgaaatatt cttagctgga
     3421 tttgatggat attcacataa tgaagatgag aattatggtg agcaatccat ggaaattata
     3481 acaaaatttg cattattaga tgcaatgaac gtgcaaatga gagatgaatt gtcagaatta
     3541 gctaaaaaag taaaggtgac atttttaacg gatccacgtt gggttcgaat tgataagtag
     3601 tagtaataat gtttttaaaa ttttgataaa gagatagtga gagaataaaa atgggtaaag
     3661 tcttaaataa aaaagaagaa aaatatttaa gggacgatgg ggcgagatga aactctaatt
     3721 ttgaactttt aagaatcatt tctatattgt gtattgttgc ttaccatgct aatttgcgtt
     3781 taaattataa tgcagacgga attatagtac atggttcatt aactgctaac cagattttct
     3841 tgaattatat tggtatttgg ggtcagctag gagttaattg ttttatattt atttcggcat
     3901 attttttaag tgagaaaaga aacattcact ttgaaaatgt tttgggaatt gtattagaat
     3961 gtatttttta tagtgtatta gtttatggaa ttctttgtgc atgtggaata gaaacatttt
     4021 cattgaacag ctttgtaaaa gaaatattat catttgctct aggtagatat tggtttatta
     4081 catcctattt agcattgtat attctaagtc cgtatttaaa tatcatgctt aacaatctgg
     4141 aaaaacttca gttgcaaaaa ataagtatgt tattgtttat aggtgtatgc tgttatcgta
     4201 tgttatttaa aacatctttg tttggggatt gtgctgtttt aattgcacta tatgtcatat
     4261 gttatacact gaaaaaaacg ataataatat ttttgagaga cacagaatca taggaacaat
     4321 tgtatgtata acagttatac cacttctcat atccatagga atacttttca aatttatacc
     4381 aaatgcaatg gttaaccaga tggttcatat tggttctcca ttgatggtta tagcaagtat
     4441 atttttattt tatgtattta aaaatttttc attttacagt aagtatataa ataaacttgc
     4501 gagaaccagt ttaggggtat atattttaca tgaaaatcat tatttcattg atggtatgaa
     4561 gtctagaggc ttaatatggg atgggatatt tggattaggc aaaaggtttt tagataggga
     4621 tttttggatt tggtttataa ttgcagttgt agttgtgtgc ataatttgca tggctatcga
     4681 tgttgtaaga gaaaaatttg aaagacagat ttttaaaatt aagagtgtaa agaacctgtg
     4741 taaacttatg aatcatgtta taaatatata cccataatta ataaatgagg agaaaagaag
     4801 tgaaagttat atcattcgga gaattattat tgagacttgc agcacccggt tattcaaagt
     4861 tatttcaaag agatagtttg gagactacat tctgcggagg ggaagctaat gtagcggtat
     4921 ccttagcaat atttggatat caatcagaat ttataacaaa gcttccggat tcagatgttg
     4981 gatatgcggc agctaatgct ttgcgttatt ttggagtaaa tgttgataat attatatttg
     5041 gagagggacg catgggactg tattatttag aaaaaggagc agctcagaga ccatctaaag
     5101 taatttatga cagaaaattt tctgcaattt cgttagctga tagtgatgaa tttaagtgga
     5161 aggagatcct aaacgatgca aattggtttc attggacagg gataaatcca gcattgtctg
     5221 agaatctctt tgagacgtcc ttagatgcat gtcaggctgc caaaaaaaga ggtataacaa
     5281 tttcttgtga tttaaattat cgatccaagc tttggacatc agaggaagca aaagaaaaga
     5341 tggaacagat actaccattg gttgatattt gtatttgtaa tgaagaagat gcagaaaaag
     5401 tgctaggaat taaagctgag catacaaata ttaatagtgg agaattaaat aaagatggct
     5461 atgtgcaggt ggctagggag attcacaaga agtttggatg taggtatgta gctacaactc
     5521 tgcgtagaag ttattcggca agtagaaatg gttggagtgc aatgttatac gaagcagaaa
     5581 aagataaagc gtatttttcc acagaataca acatacaaat tgttgatcgc gttggtggtg
     5641 gagacagctt ttctgctggt ataatatatg cgttatgtga aaatatgtcc ggacaaaagg
     5701 ctattgaatt tgcgactgca tctagttgtt taaaacatac tatagaaggt gattttaatc
     5761 gaactacaat tgaggatgta tataatttaa tgaatagcgg tggaaatggt cgcgtgttgc
     5821 gttagtcaac atataaagat aaatcacagt aatttttatg gtgattatct gtcttagtag
     5881 tgattagaat aacgtgaaaa taaattgttg agcgtttata ctgttttgaa aaataaattt
     5941 aacatattga aaaatacaaa tatatacgta tattataaag cgccgattgt aaaattaagc
     6001 tagacaacta aaacagtcat ttgtgctaca ctcaaaatag ttccgaccaa agaattataa
     6061 ggagtgagtg caaataacct atacctatct tacaacggat gaacttgtaa taatagagtc
     6121 ttatttgaaa attaaccaat ctgttgctaa aactgcgcat tgcttgaatc gttcaagtca
     6181 aacgatccat aaagtatatc tgttcttcaa gcaaagaaaa tcagccttag aata
//
LOCUS       DARBET010000111         6233 bp    DNA     linear   BCT 07-NOV-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4027
            SAMEA114569841-rid20112183.denovo.115, whole genome shotgun
            sequence.
ACCESSION   DARBET010000111 DARBET010000000
VERSION     DARBET010000111.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA114569841
            Sequence Read Archive: ERR12203666
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 6233)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6233)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001972618.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001972618.1
            Genome Coverage       :: 132x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/07/2023 13:18:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,041
            CDSs (total)                      :: 2,975
            Genes (coding)                    :: 2,908
            CDSs (with protein)               :: 2,908
            Genes (RNA)                       :: 66
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 67
            CDSs (without protein)            :: 67
            Pseudo Genes (ambiguous residues) :: 0 of 67
            Pseudo Genes (frameshifted)       :: 26 of 67
            Pseudo Genes (incomplete)         :: 28 of 67
            Pseudo Genes (internal stop)      :: 20 of 67
            Pseudo Genes (multiple problems)  :: 6 of 67
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6233
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA114569841-rid20112183.denovo.115"
                     /isolate="VRE4027"
                     /db_xref="taxon:1352"
                     /country="Denmark"
     gene            complement(<1..1477)
                     /locus_tag="SCJ86_002645"
     CDS             complement(<1..1477)
                     /locus_tag="SCJ86_002645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287057.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HEG1170133.1"
                     /translation="MKLDYLIEEEDREKLLLARYLEFEKKNYVVLPKLYETLGLSKFK
                     TRNYIIELNKEFTEFKTKPEIQISEEAEIHVERLNLTDIKNLRRIYFQRSKIARLLTE
                     IVDSKVTIEKFAENHFLSISRAYVKRRELVSFLEQHQIKLKKNILVGEEINIRNVLFG
                     VYFGVFNGFSMPFRESVMNEAKSIVQYFEYLFNLKLSATKRIKFNLLTTISLLRAYGN
                     QPIGKRFFSDEYFKSSEGMKIIKDLSHISFLHIPKEQIIDEISYIMLFLITEEEFELD
                     GAFGLAEAHFHDIHMISQKMSVELIDQLPFSIEISEEERLYIRKELRMGMMHVNRKNW
                     IFDFEMTSFTSKKQLQFFFETYPIFSTAIQKVIQENKRYFLGKDERFMIRLFYDYLFL
                     LVGICPVSYFEAPIHVCIDFSQGSAYTDYIISQVKGFKNYNIVIESRITSRTNIYLSD
                     CLLEKFNKEQIIWKNPPTPDDWEYFGNTIIREKNRHLTEKIS"
     gene            complement(1775..2710)
                     /gene="murB"
                     /locus_tag="SCJ86_002646"
     CDS             complement(1775..2710)
                     /gene="murB"
                     /locus_tag="SCJ86_002646"
                     /EC_number="1.3.1.98"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287058.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramate dehydrogenase"
                     /protein_id="HEG1170134.1"
                     /translation="MNKEEIVESLATITILFDEPLMNYTFTKTGGPADVLAFPKKQEE
                     VKQIIDYCRIHDIPWMVLGNASNLIVQDGGIRGVVIMLTEMKQIHVKGTMVIAEAGAS
                     LIDTTYAALAESLTGFEFACGIPGSVGGAVYMNAGAYGGEIKDVFAEVDLLLEDGTLK
                     TLTKDEMVFSYRHSKVQELRAIVLEARFSLQTGDYEAIKARMDELTELRQSKQPLEYP
                     SCGSVFKRPVGHYTGQLIQQAGLQGLKWGGAQVSEKHAGFIVNIDHATATDYIELIAH
                     IQEVIKEKFDVSLETEVRIIGEKQAISETSRSLAN"
     gene            complement(2783..3703)
                     /locus_tag="SCJ86_002647"
     CDS             complement(2783..3703)
                     /locus_tag="SCJ86_002647"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313435.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HEG1170135.1"
                     /translation="MKIGVIGVGNIAEKAYLPTYAKKQGTLDFYFATRNKETKERIQK
                     EYGFQHLYETIDELLEEKIEACMIHAATKVHYELAKKCLENGIHVYIDKPLSVHLNEV
                     RELQELADKNQIILMIGFNRRFAPMVEELKRIPDKRLIHLQKNRIAAKETTEFVLYDL
                     FLHLIDTAVYLLDEPILRTKYQIRETKEFLEYAILQLETQNQTAILTMDLASGANIEK
                     YQVTSKNGTYEVNDLTQLVIQEADGKKVKEFGDWTNTLVKRGFEPMVEAFFSQIRLGK
                     SETELLKQKEVVRSHELCEEVLKDHYRHQL"
     gene            3816..4568
                     /gene="xth"
                     /locus_tag="SCJ86_002648"
     CDS             3816..4568
                     /gene="xth"
                     /locus_tag="SCJ86_002648"
                     /EC_number="3.1.11.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291429.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease III"
                     /protein_id="HEG1170136.1"
                     /translation="MKFISWNVNGLRAIVNKNFLEVFHELDADFFCLQETKLQAGQID
                     LDLPGYYQYWNYAERKGYSGTAIFAKKPALNATYGMGIDIHDTEGRLITLEYSDFFLV
                     TCYTPNSQSELKRLDYRLEWEEAFYNYLENLKKQKPVIVCGDLNVAHQKIDLKNWKTN
                     QKNAGFTPEERAALSRLLDNGFIDTFRYFYPTQEGVYSWWNYRFNSRKNNAGWRIDYF
                     LTSKDLEPGLADAKIHTDIMGSDHCPVELDLK"
     gene            5432..5965
                     /locus_tag="SCJ86_002649"
     CDS             5432..5965
                     /locus_tag="SCJ86_002649"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345304.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exonuclease"
                     /protein_id="HEG1170137.1"
                     /translation="MNFVAMDFETANHQPYSACSLALVMVKNSQIVDEFYTLIQPETP
                     FFWRNVQIHGIHQEDVRNAPKFPEVWQTIQPYFQKNRLVVAHNAAFDTKVLAGCLDYY
                     HLEQPNYLSLCTVKSSRRLFPEFPNHRLNTVCENLNIQLDHHHDALEDSRACAEILLY
                     QEKKFGTDPLKKLVTIK"
BASE COUNT         1872 a         1191 c         1041 g         2129 t
ORIGIN      
        1 agctgatttt ttcagttaaa tgtctatttt tttctctgat gatggtattt ccaaaatatt
       61 cccagtcatc tggagtcggt ggatttttcc aaattatctg ttctttatta aatttttcaa
      121 gtaaacaatc agataaataa atattcgtac gagaagtgat tcgactctca atcacaatat
      181 tgtaattttt aaatcctttc acttgagaaa taatgtaatc ggtataagca gatccttgcg
      241 aaaaatcaat acagacatga atcggtgctt caaaatatga taccggacaa ataccgacca
      301 ataaaaagag ataatcataa aataagcgaa tcatgaaacg ttcatctttt cccaagaaat
      361 agcgtttatt ctcttgtatg accttttgga ttgctgtact gaatattgga tacgtctcga
      421 agaaaaactg tagctgcttt ttagacgtaa agctagtcat ctcaaagtcg aatatccaat
      481 tttttcgatt gacatgcatc atacccatac gaagttcttt tcgtatatat agtctttctt
      541 cttcagagat ttcaatcgaa aatggtaatt gatcaatcaa ttccactgac attttttgac
      601 taatcatgtg gatatcatga aaatgagctt ccgcaagtcc aaatgcgcca tctaattcaa
      661 attcttcttc cgtgatcaaa aacaacatga tataagaaat ctcatcaatg atctgctctt
      721 tagggatatg aagaaaagaa atatgtgata aatccttgat gatcttcatt ccttctgagc
      781 tcttaaagta ttcatctgaa aagaaacgtt ttccgattgg ttgattccca taggccctca
      841 acaaactaat agtggttagc aaattgaatt tgattcgttt tgttgcagac agttttaaat
      901 tgaacagata ttcaaaatat tgcacgatac tctttgcttc attcatgacg ctttctctaa
      961 atggcataga aaaaccatta aagaccccga aatatacccc aaataatacg ttccgtatgt
     1021 ttatttcttc cccaactaat atgtttttct ttaactttat ttgatgctgt tcaagaaaag
     1081 agactaattc tcttcttttt acataggctc ttgatatgct taaaaaatga ttctctgcaa
     1141 atttttcgat cgttactttt gaatccacga tctccgttaa cagtcgagca atttttgatc
     1201 gttgaaagta gattcttctt aaatttttga tatccgtcaa attcagacgt tcgacatgga
     1261 tctctgcttc ttctgaaatt tggatctctg gctttgtttt gaattcagta aattctttat
     1321 ttaattctat gatataattt cttgttttaa atttagacaa acctaatgtt tcatataact
     1381 taggcagcac tacataattc tttttctcaa attctaaata tctagctagc agcagttttt
     1441 ctctatcttc ctcttctata aggtaatcca acttcatatc ctacacctca aaaaatgcct
     1501 cataccaatt acttgatatg cagatgaaag ttcatctttt cacctatact gattttatga
     1561 tggattatgc ttgttttttc atcagttatt ctcatttttt atcttttttt tgagaatatt
     1621 gcaaaaaatg gaaaaattta tgaggaaata ttctatattt tttgttttat tgtgttaaaa
     1681 tgtacaagat ttgcgccatt tgcgtaaaca accacaaaaa agagcaggac ggccaccgtc
     1741 ctgctctttt acttatttta agttccatta cttattaatt ggctaaactt ctgcttgttt
     1801 ctgatattgc ttgtttttct ccaatgatcc gcacttctgt ttctaaagac acatcaaatt
     1861 tttctttgat tacttcttgg atgtgcgcaa tcaattcaat ataatctgtc gctgtcgcat
     1921 gatcgatatt gacgatgaat ccagcatgtt tttcagagac ttgtgcaccg ccccatttca
     1981 ggccttgcaa tcctgcttgt tggatcagct gacctgtata gtgtcctact gggcgcttaa
     2041 agacacttcc gcatgaaggg tattctaatg gctgttttga ttgacgcaat tcggtcaact
     2101 catccattct tgctttgatt gcttcataat cccctgtttg caaactgaat cgagcttcca
     2161 atacgatagc acgtaattct tggactttac tgtgacgata agaaaatacc atttcgtctt
     2221 ttgtcaaagt tttcaatgtt ccatcttcaa gcaaaagatc cacttcagca aaaacatctt
     2281 tgatctctcc gccatacgca cctgcattca tataaaccgc tccaccgaca cttcccggga
     2341 tgccgcaagc aaattcaaat ccagtcaatg attctgccaa agctgcataa gtcgtatcaa
     2401 tcagtgaggc acctgcttcc gcaatgacca tcgttccttt gacatgaatc tgtttcattt
     2461 cagtcagcat gatgactaca ccgcgaatcc cgccatcttg aacgatcaga ttgcttgcat
     2521 ttcctaatac catccaaggt atatcatgga ttcggcaata atcgatgatt tgctttactt
     2581 cttcttgttt tttcgggaat gcaaggacat cagctggtcc acctgttttc gtaaacgtat
     2641 agttcatcaa tggttcatca aacaatatag taatcgtggc taagctttca actatttctt
     2701 ctttattcac tgaggatttt cctttctttt ctcaaacgtc ctcattattc tatcacgctt
     2761 aatacaatct tgccagtctt gttcaaagtt gatgacggta gtgatcttta agcacttcct
     2821 cacataattc atgacttctt acaacttctt tttgtttaag caattctgtt tccgatttac
     2881 ctaaacggat ctgagaaaag aaggcctcta ccattggctc aaacccgcgt ttgactagcg
     2941 tgtttgtcca gtcgccaaat tctttcactt tttttccatc agcctcttga atgacaagct
     3001 gggtcagatc gtttacttca taagtaccat ttttacttgt tacttgatat ttttctatat
     3061 tagcgccact tgctaaatcc atcgttaaaa tagccgtttg attttgtgtt tctaattgta
     3121 aaatggcata ttccaaaaat tcctttgttt ctcttatctg gtattttgtt ctcaaaatgg
     3181 gttcatctaa taaatacaca gctgtatcaa taagatgtaa aaacaaatca taaaggacga
     3241 actctgtcgt ttcttttgcc gctattcgat ttttttgcag gtggatcaac cgcttatctg
     3301 ggatacgttt caattcttct accataggag caaagcgccg gttgaaaccg atcatcaaaa
     3361 ttatttggtt cttatcagct aattcttgca attcacgaac ttcatttaga tggacactca
     3421 gtggtttgtc aatataaaca tggataccat tttctagaca ttttttggct aattcataat
     3481 gtacttttgt tgctgcgtgg atcatacatg cttcgatttt ttcttctaaa agctcatcga
     3541 tcgtttcata tagatgttgg aacccgtact ctttttgaat tctttctttt gtttctttat
     3601 ttcttgttgc aaaataaaaa tcaagtgttc cttgtttttt cgcataagtt ggtaaatatg
     3661 ctttttctgc gatattccct acaccgatta caccaatctt catctacttt cctcctggat
     3721 catttagtta ctttcatctt acaccatttt ttactaaaat gaggtacaat agtatcgcaa
     3781 aggtaaggaa attaattcga aggagtgtgc ttttattgaa atttatctct tggaacgtaa
     3841 acgggcttcg tgccatcgta aataaaaatt ttttagaagt ttttcatgag ttagacgccg
     3901 attttttctg tctgcaggaa acgaaactcc aagcaggaca aattgattta gatcttccag
     3961 gttattatca atattggaat tacgcggaac gaaaaggcta ttccggcaca gcgatttttg
     4021 ctaaaaaacc agcattgaat gccacttacg gaatgggtat cgatatccat gatacagaag
     4081 gccgtctgat tactttggaa tattccgatt tctttcttgt aacctgttac acgccaaatt
     4141 ctcaaagcga attaaaacgt ttagattacc gattagaatg ggaagaagcc ttttataatt
     4201 atttggaaaa tctaaaaaaa caaaaaccag tcatcgtgtg tggggatcta aatgtcgctc
     4261 atcaaaagat tgatttgaaa aactggaaaa cgaatcaaaa aaatgctgga ttcactccag
     4321 aagaaagagc ggctctttct cgactgttgg ataacgggtt tatcgataca ttccgttatt
     4381 tttatccaac ccaggaaggt gtctactctt ggtggaacta ccgattcaat tcaaggaaaa
     4441 acaatgctgg ttggcggatc gattactttt tgacgagcaa agatttagag ccaggactag
     4501 ctgatgcaaa gatccatacg gacattatgg gcagtgatca ttgtccagta gaattagatt
     4561 tgaaataaag aggttgtgaa aaagtcgttt agtttcgagt attaagaaaa attttgtgaa
     4621 aatggctttc cacattttgc ataaatttgg cataataccg agaaactggc ttttgaacac
     4681 tgtttatcga gagattgtga aaaagctgtc ctgcatcaag taataagaac agccaaacaa
     4741 aaatagttcc tcatattttt cgtttggttg ggcttattac cgcagataca agcttttgaa
     4801 caccgtttat taagaggttg tgaaaaagct gcactgcatc aagtaataag aacagccaaa
     4861 caaaaatagt tcctcatatt tttcgtttgg ttgggcttat taccgcagat gcaagctttt
     4921 gaacaccgtt tattaagagg ttgtgaaaaa gctgcactgc atcaagtaat aagaacagcc
     4981 aaacaaaaat agttcctcat atttttcgtt tggttggact tattaccgca gatgcaagct
     5041 tttgaacacc gtttattgag aagttgtgaa atcagcgtcc tgacctgagt attagagaaa
     5101 caaatggcaa aatagttcct catattttgc tattttttga gctaataccg agggtcagct
     5161 gattgaacac cgtttagtgg agaagttgtg aaaaagtcgc ctactttcga gtcgtaagaa
     5221 atttttttga aaatggttcc tcacaacttt gcataaattt gacttaatac cgagaagtca
     5281 tgatccccat ctaactccaa atcacaagga atgagttcaa aaactagctt ttctcaacaa
     5341 atattacata aaaaattcta tgttttttgt taaatttatt tgcttttttc aaaagtatgt
     5401 tacagtaatt tcccaaggag gttttacaga aatgaatttt gttgccatgg attttgaaac
     5461 tgccaatcat cagccttata gtgcttgttc tcttgcgcta gtcatggtaa aaaatagcca
     5521 gatcgttgat gaattctata cattgataca gcctgagact ccttttttct ggcgaaatgt
     5581 ccaaattcac ggaatccatc aagaagacgt acgcaatgcg ccgaaattcc cagaagtctg
     5641 gcaaactatc caaccatatt ttcaaaaaaa tcgtttagtc gttgcacata atgctgcttt
     5701 tgatacaaag gtcttagcgg gatgcttaga ttattatcat cttgaacagc ctaattatct
     5761 ttctttatgt acagtgaaaa gtagccgtcg cctgtttcct gaatttccaa atcatcgatt
     5821 gaacactgtg tgcgaaaatt tgaacatcca attagatcat caccatgatg cattggaaga
     5881 tagtcgtgca tgtgcggaaa tcctgcttta tcaagaaaaa aaattcggga cagatccatt
     5941 gaaaaaactt gtgactataa agtaaaagaa tacttatgaa acacacaaag aggtcgtgag
     6001 cctgccgttt cactccgagt cacaaggagc aatttcaaaa aatagcttct catgtttatt
     6061 aaatattgtg actggtgacc gaggagctgg caggagaaca ccgtttattg gagaggttgt
     6121 gaaaaagctg ttctgcatca agtaataaga acagccaaac aaaaatagct tctcatattt
     6181 ttcatttggt tggacttatt accgcagatg caagcttttg gacaccgttt acc
//
LOCUS       DARBET010000112         6173 bp    DNA     linear   BCT 07-NOV-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4027
            SAMEA114569841-rid20112183.denovo.116, whole genome shotgun
            sequence.
ACCESSION   DARBET010000112 DARBET010000000
VERSION     DARBET010000112.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA114569841
            Sequence Read Archive: ERR12203666
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 6173)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6173)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001972618.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001972618.1
            Genome Coverage       :: 132x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/07/2023 13:18:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,041
            CDSs (total)                      :: 2,975
            Genes (coding)                    :: 2,908
            CDSs (with protein)               :: 2,908
            Genes (RNA)                       :: 66
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 67
            CDSs (without protein)            :: 67
            Pseudo Genes (ambiguous residues) :: 0 of 67
            Pseudo Genes (frameshifted)       :: 26 of 67
            Pseudo Genes (incomplete)         :: 28 of 67
            Pseudo Genes (internal stop)      :: 20 of 67
            Pseudo Genes (multiple problems)  :: 6 of 67
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6173
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA114569841-rid20112183.denovo.116"
                     /isolate="VRE4027"
                     /db_xref="taxon:1352"
                     /country="Denmark"
     gene            725..811
                     /locus_tag="SCJ86_002650"
     CDS             725..811
                     /locus_tag="SCJ86_002650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000493114.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system Fst family toxin"
                     /protein_id="HEG1170138.1"
                     /translation="MFELFICPLLIEVCVSLFSYWLNNRNKK"
     gene            complement(932..1621)
                     /locus_tag="SCJ86_002651"
     CDS             complement(932..1621)
                     /locus_tag="SCJ86_002651"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEG1170139.1"
                     /translation="MAFYLPYLLIFASILGIIWLSYKAYQTRYSLKGSKIRFKRFLLP
                     GVIFTLSIVLSGLFGVLEGNKRISRSILLGDITQKYEAACDKKKKEQVLTQKMEEFTT
                     CYEEMNDIFVNQEKRLTDKNMEKLTRLYQNLPEKQQKEVQDNYERTKKDVQYFKDTQT
                     EEACYNLFSDTIPFSTSEQERKERQQSVTYERYKALLQQATNIQNPTKKETALNYLKS
                     VKEWLDQQQQN"
     gene            complement(1628..1906)
                     /locus_tag="SCJ86_002652"
     CDS             complement(1628..1906)
                     /locus_tag="SCJ86_002652"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002354385.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEG1170140.1"
                     /translation="MKEEQTTNKNYRSKTLQDIENEIKKQRAFLAPYTKERIQTTIDS
                     QVKKFHHALLNQRNKEQKKVIMQRSMDILAPLCCLIMTLLAFWLVWGE"
     gene            complement(1866..2780)
                     /locus_tag="SCJ86_002653"
     CDS             complement(1866..2780)
                     /locus_tag="SCJ86_002653"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340563.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="relaxase/mobilization nuclease domain-containing
                     protein"
                     /protein_id="HEG1170141.1"
                     /translation="MATVKVSRTTSCSRAINYAEPRATVKTGINCDIDYAKSEMKQIR
                     MLYGKDDRVQAHLLIQSFRPGEITAEKANQLGAEYAKKIAPDHQIAIYTHTDKDHIHN
                     HIVINSVNLETGNKFQAHGQAFLNKCYDLNDEICLEHDLTITERGKKPEKRTMSEIKL
                     KEKNEPVWKDEIRFAIDQTMKNKKTRTYNQFCDSLKTFGIHCLNRGKNFTYELIHKKK
                     KVRSNKLGKDYEKETILHELDRRERAYNQQRTNELRRARASIRETAKEVRREQRTLTT
                     EQPEIRPTRRNHDQHVERGADYQQELSF"
     gene            complement(2762..3142)
                     /locus_tag="SCJ86_002654"
     CDS             complement(2762..3142)
                     /locus_tag="SCJ86_002654"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348702.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MobC family plasmid mobilization relaxosome
                     protein"
                     /protein_id="HEG1170142.1"
                     /translation="MSEQNQYLASNHSKKYTYRSEPKQISFRVSESEFAKLKQSAEAL
                     QMSVPAFVKAKAQNARLVTPKVAPDIAQAIARDLAKAGGNINQIAKWCNTHQHDVDPG
                     DAQRLSENLKIMQKELQKIWQQLK"
     gene            complement(3592..3879)
                     /locus_tag="SCJ86_002655"
     CDS             complement(3592..3879)
                     /locus_tag="SCJ86_002655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002363589.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bacteriocin immunity protein"
                     /protein_id="HEG1170143.1"
                     /translation="MDFTKEEKLLNAISKVYNEATIDDYPDLKEKLFLYSKEISEGKS
                     VGEVSMKLSSFLGRYILKHKFGLPKSLIELQEIVSKESQVYRGWASIGIWS"
     gene            complement(3899..4123)
                     /locus_tag="SCJ86_002656"
     CDS             complement(3899..4123)
                     /locus_tag="SCJ86_002656"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002363584.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bacteriocin hiracin-JM79"
                     /protein_id="HEG1170144.1"
                     /translation="MKKKVLKHCVILGILGTCLAGIGTGIKVDAATYYGNGLYCNKEK
                     CWVDWNQAKGEIGKIIVNGWVNHGPWAPRR"
     gene            complement(4578..4748)
                     /locus_tag="SCJ86_002657"
     CDS             complement(4578..4748)
                     /locus_tag="SCJ86_002657"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322958.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEG1170145.1"
                     /translation="MTKKHLLFGSILLGILSFSPMLLSVANKGNKKGELKLNPNYYNS
                     QNEEVFVEKLDK"
     gene            complement(4763..5296)
                     /locus_tag="SCJ86_002658"
     CDS             complement(4763..5296)
                     /locus_tag="SCJ86_002658"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292473.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF536 domain-containing protein"
                     /protein_id="HEG1170146.1"
                     /translation="MQPQETFTIKELSDLFKMSRQAISKHIQKLDSSMIAKNERGYKV
                     VLRSGVLQLARNLDNQRLLDKLHKHEQNNDKNAYIPNKNVVANKLLDQLREENEFLRE
                     QLALKEQNNSHIQKLLDQQQQLTLQANQQIEKLQEQLQLTYTEESSENSSTTLSEATE
                     TMDEQTKNWKKWWQLWK"
     gene            complement(5317..6159)
                     /locus_tag="SCJ86_002659"
     CDS             complement(5317..6159)
                     /locus_tag="SCJ86_002659"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321385.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation protein"
                     /protein_id="HEG1170147.1"
                     /translation="MREKGDKTVRFTFDQLKDLSNYKATANVRFVEDLKATYEKILSL
                     RFGRRSESGLSYDMFVMFTEFKINGDVENPYVDIRIYEKALPLLNNLDEWVRYSLQQF
                     NELQSGYAKTMFRLLKQYRTKGFAYFSKEDFLELLDIPKSYKQPDIDKRVIKPIRQEL
                     TAIFKGLTIKKKYGKGRGKPVIGYQFTFKPEIKKADDFNKHAPTKEDQNKALALADKQ
                     KEYEEPATTINEKIKQKKRELELLQQIKQLEKEKKALEKQTESALSEKEKKSLLSDLD
                     NLFQ"
BASE COUNT         1633 a         1152 c         1042 g         2346 t
ORIGIN      
        1 aaaaaaacaa attcatttca accggtgtaa attttcttaa aggaattgta tttaattcat
       61 ggtggtattt tacaatctca ttagccatat aatcacctca atccaattat gaatgacatt
      121 ttttaaatgt caagacattc atatttgacg ttcaaacaga caaaatgtcc ttcataaaca
      181 gacaaaatgt ccttcataaa cagacaaaat gtccttcata aacagacaaa atgtccttca
      241 taacattctg tatccattgg tagagtaagg acacagctta cggtaaagag gtttaaaaag
      301 aggtttaaaa agagatataa aaagaggggg gctaattttc ccgcctaaaa accacttctt
      361 ttttctttaa acaggttcgc gcatttttca aaactgccaa tccaaaacct tgcttcctac
      421 ggtcgccaaa aaggtgtgat cgctacgctc tcacattgta ctgcgcagtc gcctaaaggc
      481 tcctttgcta aaaacatgtt ggtaaagagg cctttgagcg gttctgaatg cttatctaag
      541 ccgttttaag acagtcttgg ataaaaatcc ttatctagct ttaaagcgcc ttacaggcgg
      601 tcagggcaag ccctaacaac cctaaaataa gcgatactta aaaacaccac acaagtttaa
      661 ttttttaaga tataataact tgtcgcctat ccgacagtct tgcgactgga aggaggtgta
      721 tctaatgttt gaattgttca tttgccccct tcttatagaa gtttgtgtct ctttattcag
      781 ttattggttg aataaccgaa acaagaagta aaatcaataa aggcgacatc aacctgaaaa
      841 aaagccccag agaatccgac ctctctgggg ctttcgtgta tctaatgttt gatcatttgc
      901 atgtttagtt taacatgatt gccgatcaaa ttcaattttg ctgttgctga tctaaccatt
      961 ccttgacact ttttagataa tttaatgccg tttctttttt cgttggattt tgtatgttcg
     1021 ttgcttgctg aagcagtgct ttataacgtt cgtaggtaac tgattgttgc cgttcttttc
     1081 gttcctgttc actagttgaa aaagggattg tatcactgaa taaattataa caagcttctt
     1141 ctgtctgcgt atctttaaaa tattgcacgt cttttttggt tcgctcatag ttgtcttgaa
     1201 cctctttttg ctgtttttct ggcaagtttt gataaagacg agtgagtttt tccatgtttt
     1261 tatccgtcaa acgtttttct tgattcacaa aaatatcgtt catttcctca taacaagtgg
     1321 taaactcttc cattttttgt gttaacactt gctccttttt ctttttatca catgctgctt
     1381 catacttttg agtgatatcc cctaataaaa tacttcttga aatacgttta tttccctcta
     1441 aaacgccgaa taaaccactt aaaacgattg ataatgtaaa tataacccct ggcaacaaaa
     1501 acctcttaaa tcgaatttta gagcctttta atgaatatct tgtttgataa gccttatagc
     1561 taagccaaat aattcctaaa atactggcaa aaatgagtag atagggtaaa tagaatgcca
     1621 tttctattta ttctccccat accagccaaa aggctaacag tgtcatgatt aagcaacata
     1681 agggggcaag aatatccatt gatcgctgca taattacttt tttctgttct ttatttcgct
     1741 ggtttaatag cgcatggtgg aattttttga cttgtgaatc aattgtagtt tgtattcttt
     1801 ctttggtata aggtgcaaga aaagcacgtt gtttctttat ttcattttct atgtcttgta
     1861 acgttttaga acgataattc ttgttggtag tctgctcctc tttcaacgtg ttggtcatgg
     1921 tttcttcgag tcggtctaat ttccggttgt tcagtcgtaa gagttcgttg ttctcttcgt
     1981 acttcttttg cagtttcgcg aatgctagct cgagctcttc gtagctcgtt ggttcgttgt
     2041 tgattgtagg ctcgttctcg tctgtccagc tcatgtaaaa tcgtctcctt ttcgtaatcc
     2101 tttcctagtt tgttggatcg tacttttttc tttttgtgga tcaattcgta agtaaaattt
     2161 ttcccacgat tcaagcaatg aatgccgaat gttttaagag aatcacaaaa ttggttatac
     2221 gttcgagttt ttttgttttt cattgtttga tcaatcgcaa accgaatttc gtctttccaa
     2281 acaggttcat ttttttcttt taatttgatt tcagacatgg ttcgtttttc gggtttcttc
     2341 cctcgttcag tgattgttaa gtcatgttca agacaaatct cgtcatttag gtcataacat
     2401 ttgtttaaaa atgcttgacc atgggcttga aatttgttcc cagtttccag attgacggag
     2461 ttaatgacaa tgtggttatg aatatggtct ttgtctgtat gtgtgtaaat cgcgatttgg
     2521 tggtcagggg ctattttttt cgcatactct gcccctagtt gattggcttt ttcagcggta
     2581 atttctcctg gtcgaaaaga ctggatcaat aagtgtgctt gtacacgatc gtcttttcca
     2641 tacagcatgc gaatttgttt catttcgctt ttggcataat caatgtcaca gtttatccct
     2701 gttttgacgg ttgctcgtgg ttcggcgtaa ttgatcgctc ggctacaaga agtggttcgg
     2761 cttactttaa ctgttgccat atcttttgta gctccttttg catgattttt aggttttcgg
     2821 ataagcgttg ggcgtcgcca ggatcaacgt cgtgttggtg tgtgttgcac cacttggcaa
     2881 tttgattgat attcccgcca gctttggcta aatcgcgcgc gattgcctga gcaatgtctg
     2941 gcgccacttt cggtgtcacc agacgcgcgt tttgtgcctt ggctttcacg aaagccggca
     3001 cactcatttg caaagcttca gctgactgct tcagctttgc aaattcggat tcgctcactc
     3061 gaaaactgat ttgttttggc tcggatcgat aggtatattt tttagaatga ttgctagcca
     3121 aatattgatt ttgttcgctc ataagtccca ccctttcagt acgtcctacg gacgtatcgt
     3181 tcgcaatacc attgtaccta aaaaggtaca ataagtaaag ctcactaaag ctcactaaag
     3241 ggcttggcaa gctctatatt tgctagccaa atacctacgt atttggtgca aataaccgct
     3301 tgtcaaaggg gcgcaagctc ccccttgaat ccccctcccc cccactctcc acaatacaaa
     3361 aaagactagc aaatgctagc cactttttgt aaagtttcgc ttgtttcacc ttcggtgaaa
     3421 ttgttgcaaa tcaataatta ggtagagcta catttttttc gttgttcaaa gattcttatg
     3481 ccatttacta ttaataaatc tataaatatg ataatttttc ggctctataa tttatgggac
     3541 tgatgaatca gaattgattc ttcagtccca ttttcatttt attccaaaga attaactcca
     3601 aataccaata gaagcccatc ctctatatac ttgagattcc ttactaacaa tttcttgtaa
     3661 ttctattaaa gatttaggta atccaaattt atgttttaaa atatatcttc caagaaaact
     3721 acttaatttc atactaactt caccaacact ttttccctca ctgatttctt tagaataaag
     3781 aaagagcttt tcttttaagt caggatagtc atctatagtt gcttcattgt atactttact
     3841 aattgcattt aaaagttttt cttctttagt aaaatccatt ataataacct ccctaccact
     3901 accttctagg tgcccatgga ccatgattaa cccaaccatt aacaataatt tttccaattt
     3961 ctcctttagc ttgattccaa tctacccaac atttttcttt gttacaataa agaccatttc
     4021 catagtaagt agctgcatca acttttattc ctgtaccgat gccagctaga caagttccta
     4081 atattcctag aataacacaa tgttttaata ctttcttttt catgattgtt tttccttttc
     4141 gtttttttaa aattgcttgt tatattaaat aatcttagta attttattat ataacatttt
     4201 attatataac aatgattaac gtcataaaaa gtagttttga attcataatc gtttaaataa
     4261 aattataaga tcagttattt aaacagctag tagacataat gtgtatacta ccaagtaacg
     4321 cctgtaccaa gggatccagg cgctgatttt gtgaattttt attggtctag gttaaaagct
     4381 ctttttaagg cttggttttc tgcggttgtt tctttctaat tctttgtgaa tattgatcgg
     4441 tcatttttta aatacttgtt atttttgttt gtgtttgtta tgctttttac atatctcata
     4501 ttcatttatt attcttttct aaggttaata ataggataat ctctttcaga aagactgctc
     4561 gtacgagcag tcttttctta tttatcaagt ttttctacaa atacttcctc attttgactg
     4621 ttgtagtaat taggattgag ttttagttcc ccttttttat ttcctttgtt ggccactgat
     4681 aataacattg ggctaaagct taatattcct agtaggatag aaccgaatag caagtgtttt
     4741 tttgtcataa gtatttcctt cttcatttcc atagctgcca ccattttttc caattctttg
     4801 tttgttcgtc cattgtttct gttgcttcag aaagagtcgt tgaactattc tctgaagact
     4861 cttccgtata agtcaactgc agttgctcct gaagtttttc gatttgttgg ttggcttgca
     4921 gggtgagttg ttgttgctgg tcgagtagtt tttgtatatg agaattattt tgctctttga
     4981 gggcaagttg ttctcgtaaa aattcatttt cttcacgcaa ctgatctagc aacttgtttg
     5041 caaccacgtt tttgtttggt atataagcat ttttatcgtt gttttgttca tgtttatgca
     5101 acttatctag caacctttga ttgtctaagt tgcgagcaag ttgcaatact ccagaacgca
     5161 atacaacctt ataaccacgc tcatttttag ctatcatgga agaatctaat ttttggatat
     5221 gtttcgatat tgcttgccta ctcatcttaa aaagatctga caattccttt attgtaaagg
     5281 tttcttgtgg ttgcatactg gttgcctcct aggttgctac tggaataaat tatctaaatc
     5341 agataataaa ctttttttct ctttttccga aagtgcggac tctgtttgtt tttcaagagc
     5401 ttttttctct ttttctagtt gtttgatttg ttggaggagt tcaagttctc gtttcttctg
     5461 tttgattttt tcgttaattg ttgtagcggg ttcttcgtat tctttttgtt tatcagctaa
     5521 agccaatgct ttattttggt cttctttggt tggtgcatgt ttgttaaagt cgtctgcttt
     5581 tttgatctca ggtttgaacg taaattgata gccaataaca ggttttcccc gtccttttcc
     5641 gtatttcttt ttgattgtta gtcctttgaa gattgcagtt agttcttgac gtataggttt
     5701 tataactcgt ttatctatat caggttgctt ataactttta ggaatatcta ataattcaag
     5761 aaagtcttct ttggagaaat atgcgaaacc tttggttctg tattgcttta acagacgaaa
     5821 catagtcttt gcatagccac tttgtagctc gttgaattgt tgtaaagaat agcgtaccca
     5881 ctcgtctaaa ttatttaaca aaggcagtgc tttttcgtaa attcgtatat ctacgtaagg
     5941 attttctacg tccccgttga ttttaaattc tgtaaacatt acaaacatat cgtaagaaag
     6001 cccagactca cttctacgac caaagcgtaa agataagatt ttttcataag ttgcttttaa
     6061 atcttctaca aatcgtacat ttgcagttgc tttgtagtta cttaaatctt ttagttggtc
     6121 aaacgtaaaa cgaactgtct tatccccttt ttcacgcatt ctagacacga tgg
//
LOCUS       DARBET010000113         6159 bp    DNA     linear   BCT 07-NOV-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4027
            SAMEA114569841-rid20112183.denovo.117, whole genome shotgun
            sequence.
ACCESSION   DARBET010000113 DARBET010000000
VERSION     DARBET010000113.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA114569841
            Sequence Read Archive: ERR12203666
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 6159)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6159)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001972618.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001972618.1
            Genome Coverage       :: 132x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/07/2023 13:18:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,041
            CDSs (total)                      :: 2,975
            Genes (coding)                    :: 2,908
            CDSs (with protein)               :: 2,908
            Genes (RNA)                       :: 66
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 67
            CDSs (without protein)            :: 67
            Pseudo Genes (ambiguous residues) :: 0 of 67
            Pseudo Genes (frameshifted)       :: 26 of 67
            Pseudo Genes (incomplete)         :: 28 of 67
            Pseudo Genes (internal stop)      :: 20 of 67
            Pseudo Genes (multiple problems)  :: 6 of 67
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6159
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA114569841-rid20112183.denovo.117"
                     /isolate="VRE4027"
                     /db_xref="taxon:1352"
                     /country="Denmark"
     gene            101..2542
                     /locus_tag="SCJ86_002660"
     CDS             101..2542
                     /locus_tag="SCJ86_002660"
                     /EC_number="1.1.1.88"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853299.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxymethylglutaryl-CoA reductase,
                     degradative"
                     /protein_id="HEG1170148.1"
                     /translation="MKEVVMIDAARTPIGKYRGSLSPFTAVELGTLVTKGLLDKTKLK
                     KDKIDQVIFGNVLQAGNGQNVARQIALNSGLPVDVPAMTINEVCGSGMKAVILARQLI
                     QLGEAELVIAGGTESMSQAPMLKQYQSETNEYGEPISSMVNDGLTDAFSNAHMGLTAE
                     NVATQFSVSREEQDRYALSSQLKAAHAVEAGVFSEEIIPVKISDEDVLSEDEAVRGNS
                     TLEKLGTLRTVFSEEGTVTAGNASPLNDGASVVILASKEYAENNNLPYLATIKEVAEV
                     GIDPSIMGIAPIKAIQKLTDRSGMNLSTIDLFEINEAFAASSIVVSQELQLDEEKVNI
                     YGGAIALGHPIGASGARILTTLAYGLLREQKRYGIASLCIGGGLGLAVLLEANMEQTH
                     KDVQKKKFYQLTPSERRSQLIEKNVLTQETALIFQEQTLSEELSDHMIENQVSEVEIP
                     MGIAQNFQINGKKKWIPMATEEPSVIAAASNGAKICGNICAETPQRLMRGQIVLSGKS
                     EYQAVINAVNHRKEELILCANESYPSIVKRGGGVQDISTREFMGSFHAYLSIDFLVDV
                     KDAMGANMINSILESVANKLREWFPEEEILFSILSNFATESLASACCEIPFERLGRNK
                     EIGEQIAKKIQQAGEYAKLDPYRAATHNKGIMNGIEAVVAATGNDTRAVSASIHAYAA
                     RNGLYQGLTDWQIKGDKLVGKLTVPLAVATVGGASNILPKAKASLAMLDIDSAKELAQ
                     VIAAVGLAQNLAALRALVTEGIQKGHMGLQARSLAISIGAIGEEIEQVAKKLREAEKM
                     NQQTAIQILEKIREK"
     gene            complement(2872..3576)
                     /locus_tag="SCJ86_002661"
     CDS             complement(2872..3576)
                     /locus_tag="SCJ86_002661"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEG1170149.1"
                     /translation="MKKKQKQKLLNQFRPSLDGVRGQLFRSLEEESLRRYGLPLQVML
                     DPKNRQILTIGLAGKTNEDTRINVPIDDNFNTVLKRIRSGENGIFERFRDNLLIEIVS
                     YWNDQRLKNDEPTAQAVATPVTEETVSVPEEKTEKPKADTTENTRSTDTNAALSFADF
                     SKAVAEYPKFYTEQNEHEVVINEKNADEPRKLATISMTTENEFVIEKALERKYKVKLT
                     LIPLIEQFAATAITDR"
     gene            complement(3763..4008)
                     /locus_tag="SCJ86_002662"
     CDS             complement(3763..4008)
                     /locus_tag="SCJ86_002662"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEG1170150.1"
                     /translation="MDPIERLNSLSEEVTQTFHSDFVFLIDAEKIQHFPARNWTHDQI
                     IEELKKRFDHSLMVKPWHEHEVIYSPELPVFALIPKK"
     gene            complement(4025..4876)
                     /locus_tag="SCJ86_002663"
     CDS             complement(4025..4876)
                     /locus_tag="SCJ86_002663"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735800.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HEG1170151.1"
                     /translation="MNSLTSTYRLSNGYEIPVVGFGTWQTPDGDVAVSSVKEALAAGY
                     RHIDTAQGYKNEESVGQAIKESGIPREEIFLTTKLWNANHSYELVMSSFEESLKKLQT
                     DYLDLFLIHWPNPVAFRDNWEQANADTWRAFEELYEAGKIKAIGVSNFLPHHLDTLAK
                     TAKIMPMVNQVFLAPGELQTTVVEYAKKHDMILEAYSPLGTGKIFDVPEMKQIAEAHD
                     KTIAQVALRWSLQHEFLPLPKSVTPSRIKENTELFDFELTEEEMKQIDQLDGVVGKAK
                     DPDTTQF"
     gene            5042..6046
                     /locus_tag="SCJ86_002664"
     CDS             5042..6046
                     /locus_tag="SCJ86_002664"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735799.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoid-associated protein"
                     /protein_id="HEG1170152.1"
                     /translation="MDIYLKKAILHIIDRETGSPVFSQKELDLTKEYIRDFLQKKIQK
                     ISSAQTKTGQLAEESMIVESLKIAQENFVEASEAIVQRWFDIYQESEEAPSADVFVVL
                     YELDTVMYLSLLKVNYREAYTHFVEADEAGIDNRLILNRAILGAKTQKADEAFAVNLT
                     DWTYELIEKKYEFSGKKELYFSGRVIESVPAPSLEENVKVIQKVAKHLGKKFETEEFD
                     IMADLKEAVYDTIEEKGRLDHEMIAEKVFKENITAKMAFKEEVQEQGFVPEAPPVKEV
                     QEISEKKFGKQKFKLSNGIELIVPVDVYRNPDLIEFVNNPDGTISVMIKNVDEVLNRL
                     "
BASE COUNT         1979 a         1065 c         1303 g         1812 t
ORIGIN      
        1 ttaaggattg ttaaaattta atcttagaaa aaaataactg gaacaagagc tatttgtttt
       61 tacagcaaag aatggtatat ttttgtagga ggtggcttac ttgaaagaag tcgttatgat
      121 agatgctgca agaacgccga taggaaagta tcgcggaagt ttaagtccat ttactgcagt
      181 tgaattaggc acacttgtga ctaaaggatt attagataaa acaaaactga aaaaagataa
      241 aatcgatcaa gtaatatttg gaaatgtcct gcaagctggt aatggtcaaa atgttgccag
      301 acagattgct ttgaacagcg gtctccctgt agatgtccca gcaatgacga tcaatgaagt
      361 ttgcggatct ggtatgaaag cagtgatctt agcacgccag ctgatccaat taggtgaagc
      421 agaacttgtg atagccggcg gaacagagag tatgtctcaa gcacctatgc tgaaacagta
      481 tcagtcagaa acaaatgaat atggtgaacc aatttccagt atggtcaacg acggattgac
      541 tgacgcattt tcaaatgcac atatgggatt aaccgcagag aatgttgcaa cacaattttc
      601 tgtgagcaga gaagagcagg atcgctatgc cttgtcgtcc cagttgaaag cagcacatgc
      661 tgtcgaagcc ggtgtatttt ctgaggagat catcccagtc aagatttctg atgaagacgt
      721 gttatctgag gatgaagcag ttcgtggaaa tagtacattg gaaaaactgg gcacgttacg
      781 tacagtattc tcagaagaag gaactgtaac agcaggaaat gcttccccgt tgaatgacgg
      841 tgcctctgtg gtgatccttg catccaaaga atacgcagaa aataataatc tgccttattt
      901 agcaaccatc aaagaagtag cggaggtcgg tattgaccca agtatcatgg gaatcgctcc
      961 aatcaaagcc attcaaaaat tgacagatag atcaggaatg aatttatcaa caatcgatct
     1021 ttttgagatc aatgaagcat ttgcagcttc ttctatcgta gttagccaag aactgcagtt
     1081 agatgaagaa aaggtcaata tatatggagg agcaatcgca ttaggccatc caattggtgc
     1141 aagcggtgca cgtatcttga ctacattagc ttacggattg cttcgtgaac agaagagata
     1201 cggtattgct tcattatgta ttggtggagg tttaggcttg gcagtcttgt tagaagcgaa
     1261 tatggagcaa acacataagg atgtgcaaaa aaaaaagttt tatcagctta cgccatctga
     1321 acggcgcagt caattaatag aaaaaaatgt attgacacaa gaaactgcgt tgatttttca
     1381 ggaacaaaca ctttctgaag agctctccga tcacatgatc gaaaaccaag tgagtgaagt
     1441 ggaaatccca atggggatcg cgcagaattt tcaaatcaat ggcaagaaaa aatggattcc
     1501 gatggcaaca gaggaaccat ctgtcattgc agctgcaagt aatggtgcaa aaatctgtgg
     1561 aaatatctgc gcagaaacac ctcagcggct gatgcgaggg caaatcgttc tatcaggaaa
     1621 atcggagtat caagcagtga tcaatgcagt taatcatcga aaagaagagc tgattctttg
     1681 tgcaaatgaa agttatcctt caatcgtgaa acgcgggggc ggtgttcaag atatctctac
     1741 tcgggaattc atggggagtt tccatgcata cttatctatt gattttttag tggatgtaaa
     1801 agatgcaatg ggcgctaaca tgatcaactc gattcttgag agtgtggcaa acaagttacg
     1861 ggagtggttc cctgaagaag agattctatt cagtatttta agtaattttg ctacagaatc
     1921 attggcttct gcctgttgtg aaatcccatt cgaacgctta gggagaaaca aagaaatcgg
     1981 tgaacaaatt gccaaaaaaa tccaacaggc aggggaatat gcgaagcttg acccttaccg
     2041 tgcagcaacg cacaacaaag ggatcatgaa tggtatagaa gctgtagtgg cagctacagg
     2101 aaatgataca cgtgcggtta gcgcttcgat ccacgcctac gcggcaagaa atggattata
     2161 ccaaggatta acagactggc agatcaaagg ggataaacta gttggaaaac taacggtgcc
     2221 tttggctgtt gccacagtag gcggagcttc caatatcctg ccaaaagcaa aagcatcttt
     2281 agctatgtta gatattgatt cagccaagga actagcacaa gtaattgctg cagtagggtt
     2341 agctcaaaac ctggctgctt tgcgtgcatt agttacagaa ggaatccaaa aaggccacat
     2401 gggacttcaa gctcgttcat tagcgatctc aattggggct attggtgagg aaatcgagca
     2461 agttgcgaaa aaattacgtg aagcagaaaa gatgaaccag cagacagcta tacaaatatt
     2521 ggaaaaaatc cgggaaaaat aaaaaagcaa tatctttaga agaataaagc aagatttaca
     2581 gttattttat ttactgcaga tcttgctttt ttctctatca gaaaatatgt ttattattag
     2641 aattttttat agttaaggtt aggcatctta agaaatcagg caactccaag atatcttcag
     2701 gtagaagaga agtcgcctaa cattgtagga tcataggtga tttgttggac aggtcaagct
     2761 gatatttgag cctgatgtta gaaggaattt ctttttactt gctgtagaaa ttattatatg
     2821 actaaaaagg ctgggatttt aaatcccagc cttttatgac aaatagaatt tttatcgatc
     2881 agtaatcgct gtcgctgcaa attgttcgat caaaggaatc aaagtcaatt tgactttgta
     2941 tttccgttcg agtgcttttt cgataacgaa ctcattttca gtagtcatag aaatagttgc
     3001 taatttacga ggttcatcag catttttttc attaataact acttcatgtt cattttgttc
     3061 tgtgtaaaat ttaggatact cagcaacggc ttttgaaaaa tcagcaaaac ttaaagcggc
     3121 attcgtatca gttgagcgtg tattttcagt tgtgtccgct tttggttttt ccgttttttc
     3181 ttctggcaca ctgactgttt cttctgtaac tggtgtcgca acagcttgcg cagttggttc
     3241 atcgtttttc aatcgttgat cattccagta agacacgatt tcaataagaa gattgtcacg
     3301 gaatcgttcg aaaatcccgt tttctcctga acgaattctc tttaatacag tattgaagtt
     3361 gtcatcgatt ggcacgttga tccgtgtatc ttcatttgtt ttcccagcta atccgatcgt
     3421 taagatttga cggtttttgg gatcaagcat gacttgtaac ggcaaaccat aacgtcgcaa
     3481 gctctcttct tctagagaac ggaataattg tcctcttaca ccatccaatg aaggacggaa
     3541 ttgatttaat aatttttgtt tctgtttttt cttcatatgt tctctccttc gctcataccc
     3601 tttcgattat aagaaaaaaa cagccaaatg aaaaggagta atgcaaaaaa acttgcgtat
     3661 acgcaaatta caacagaaaa atttgcgtat tttcccgttt tttgaaagaa aaataaaaaa
     3721 tggaacggat tatgtatata tacataatcc gttccattct ttctattttt tcggtatcaa
     3781 ggcaaaaaca ggtagttcag gtgaataaat gacttcatgt tcgtgccagg gtttgaccat
     3841 tagcgaatga tcgaaccgtt ttttcagttc ttcgatgatt tgatcatgtg tccagtttct
     3901 tgcaggaaaa tgttggattt tctctgcatc gatcaaaaag acaaaatcgg aatgaaaagt
     3961 ctgggtaact tcttccgaga gagaattcaa gcgttcgatt ggatccataa aatcaccttt
     4021 cgacttaaaa ttgggtagta tctgggtctt ttgctttgcc gactacacca tctaactgat
     4081 cgatttgttt catttcttct tctgttaatt caaaatcaaa taattcagta ttttctttga
     4141 ttctgcttgg tgtcactgac ttaggaagag gaaggaattc gtgttgcagc gaccaacgca
     4201 aagcaacttg tgcaatcgtc ttgtcatgtg cttcagcgat ttgtttcatt tcaggcacat
     4261 cgaagatttt accagtgcct aatggactat atgcttcgag gatcatatca tgtttttttg
     4321 catactcaac tacagttgtc tgtaattcgc ctggtgcaag gaaaacttga ttgaccatcg
     4381 gcataatttt tgctgttttt gctagtgtat ctagatggtg gggtaagaaa ttgctcacac
     4441 cgattgcttt gattttacct gcttcataca gttcttcaaa tgctcgccat gtatctgcat
     4501 ttgcttgttc ccaattatca cgaaaagcaa cagggtttgg ccagtggatc aaaaacagat
     4561 ctaaataatc tgtttggagc ttcttcaatg attcttcaaa agaagacatc acaagttcat
     4621 agctgtggtt tgcattccat aattttgttg tcaagaatat ctcttctcta ggaattccac
     4681 tttccttaat cgcttggccg acgctttctt cattcttgta tccttgggca gtatcgatat
     4741 ggcgataacc tgcagccagc gcttctttaa cagaggaaac agcgacatcc ccatcaggtg
     4801 tttgccatgt gccgaatcct actaccggga tttcgtaacc atttgataga cgataagtgc
     4861 ttgttaatga attcatactt tcagctcctt ttttattcgt tcaactataa gtgtagcgca
     4921 tttttcgaaa aaaagaatcc attttgtttc tactcttgaa atttttgttt aaaagaaaat
     4981 gattttgatt ctggtatttt tgattttttt tccgtaaact aagacagaag ggaatgagaa
     5041 aatggatatc tatttaaaaa aagcaattct gcatattatt gacagagaaa ctggttctcc
     5101 agtcttttca caaaaagagc tggatctgac aaaagaatat attcgtgatt ttcttcaaaa
     5161 gaagatccaa aaaatctctt ctgctcaaac aaaaactgga caactagcag aagaaagcat
     5221 gatcgttgaa tcacttaaaa tcgctcaaga aaattttgtg gaagcaagtg aagcaatcgt
     5281 tcagcggtgg ttcgatatct accaagaaag tgaagaagca cctagtgcag atgtatttgt
     5341 tgtcttgtat gaattagata cagtgatgta cttatcttta ttgaaagtca attatcgtga
     5401 ggcttacact cactttgttg aagcagacga agcagggatc gacaaccgct tgattttgaa
     5461 tcgagcaatt ctaggggcga aaacacaaaa agcagatgaa gcattcgctg tgaatttgac
     5521 tgattggacc tacgaattaa ttgaaaaaaa atatgagttt tctggcaaaa aagagcttta
     5581 tttttctgga agagtgatcg aaagtgttcc tgcaccttct ttagaagaaa atgtaaaagt
     5641 gatccaaaaa gtagccaagc atcttggaaa aaaatttgag acagaagaat ttgatatcat
     5701 ggctgatttg aaagaagctg tctatgatac gatagaagaa aaaggtcggt tggatcatga
     5761 gatgattgcg gaaaaagtct tcaaggaaaa catcacggca aaaatggctt ttaaagaaga
     5821 agtacaagag caaggatttg tcccagaagc acctcctgta aaagaagtac aagaaatttc
     5881 tgagaaaaaa tttggaaaac aaaaattcaa gctatcaaac gggatcgaac tgatcgtacc
     5941 agtagatgta tatcgcaacc cagatttgat tgaatttgtg aataatccgg atggtacgat
     6001 atccgtgatg atcaaaaatg tggatgaagt attgaatcgt ttataaaaat gactagaata
     6061 ttaggtgttc aagtaccagt gatcagttaa atgaattgtg tcaagagaag gatcggaaaa
     6121 gacgaatata acaaaaaaag agcgtgagac aaaagtaaa
//
LOCUS       DARBET010000114         6123 bp    DNA     linear   BCT 07-NOV-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4027
            SAMEA114569841-rid20112183.denovo.118, whole genome shotgun
            sequence.
ACCESSION   DARBET010000114 DARBET010000000
VERSION     DARBET010000114.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA114569841
            Sequence Read Archive: ERR12203666
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 6123)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6123)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001972618.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001972618.1
            Genome Coverage       :: 132x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/07/2023 13:18:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,041
            CDSs (total)                      :: 2,975
            Genes (coding)                    :: 2,908
            CDSs (with protein)               :: 2,908
            Genes (RNA)                       :: 66
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 67
            CDSs (without protein)            :: 67
            Pseudo Genes (ambiguous residues) :: 0 of 67
            Pseudo Genes (frameshifted)       :: 26 of 67
            Pseudo Genes (incomplete)         :: 28 of 67
            Pseudo Genes (internal stop)      :: 20 of 67
            Pseudo Genes (multiple problems)  :: 6 of 67
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6123
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA114569841-rid20112183.denovo.118"
                     /isolate="VRE4027"
                     /db_xref="taxon:1352"
                     /country="Denmark"
     gene            complement(197..1159)
                     /locus_tag="SCJ86_002665"
     CDS             complement(197..1159)
                     /locus_tag="SCJ86_002665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004233175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HEG1170153.1"
                     /translation="MVAKLTDVAELAGVSPTTVSRVINNKGYLSEKTKQKVHEAMKTL
                     GYKPNNLARGLQGKSPQLIGLIFPNISNIFYSELIEYLEIELFKHGYKTIICNSQNDP
                     AKEREYIEMLEANQVDGIISSSHNLGIADYERVRAPIVAFDRNLAPNVSIVSSDNFEG
                     GKLAAKTLQKNGCQNTIMITGNDNTDSPTELRALGFSFQNPNGKIFKIPNNLSTIRRE
                     MEIKSTIINHKPDGIFVSDDLTAILTMKIAHQLKLNIPEDLKIIGYDGTSFIEKFFPQ
                     LTTIRQPIDEIASLIVDILLKKIKGEKTNKDYILPISILSGGSI"
     gene            complement(1161..2600)
                     /locus_tag="SCJ86_002666"
     CDS             complement(1161..2600)
                     /locus_tag="SCJ86_002666"
                     /EC_number="3.2.1.26"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010737355.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sucrose-6-phosphate hydrolase"
                     /protein_id="HEG1170154.1"
                     /translation="MNLPREIRYRAYSDWTKDEMDKIKDNVKRSPWRASYHIEPKTGL
                     LNDPNGFSFFNGKYTLFYQNWPFGAAHGLKEWVHNESDDLVHFHVTGAELLPDTKRDS
                     HGAYSGSAYEIEGNLFLLYTGNVRNENWERDTLQIGAWMDKDYNITKGENVLIHKPAD
                     VTDHFRDPQMFNYEGQLYAIVGAQNLEKSGFIKLYKADDNNVETWEEVGNLDFGGTGS
                     EYMIECPNLVFVDKKPVLIYCPQGLDKSELNYGNIYPNTFKICQSLDIEKVKLVGASE
                     IQNLDYGFEAYATQGFNTPDGRALIVSWIGLPDIDYPTDKYDYQGAMSLVKELSIKDG
                     KLYQYPVEAITSLRASSEQFTAKAETNNTYELELTFPPNQKSELLLFSDSKGNGLSLI
                     VDTKEGKIILDRSKAGIQYATDFGTIRECSIDTKETTANIFVDNSIIEIFINKGEKVF
                     TSRVFPENHQNGIQIKSGEPTGKYFELKY"
     gene            2785..4731
                     /locus_tag="SCJ86_002667"
     CDS             2785..4731
                     /locus_tag="SCJ86_002667"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS beta-glucoside transporter subunit IIBCA"
                     /protein_id="HEG1170155.1"
                     /translation="MNYSKVASDVIQAVGKDNLIAAAHCATRLRLVLKDDTKVNQKAL
                     DENPDVKSTFKINGQYQVIIGAGDVNFVYDELIKKTGLSEISTDDLKQIASKDKKFNP
                     IMALIKLLSDIFVPIIPALVAGGLLMALQNFLTAAGLFGPKSIEEMYPAIEGISSMIQ
                     LMSAAPFMFLPILVGISAAKRFGANQFLGAAIGMIMTTPDLGGSTEYWNIFGYHVSQT
                     NYAYQVIPVLAAVWILSILEKFFHKKLPSSIDFTFTPLLSVMITGFLTFTVIGPVMLL
                     LSNGITDIIVWLYNTTGFIGMGIFGGTYSLIVMTGLHQSFPAIETQLLSAWTNGIGHG
                     DFIFVVASMANVAQGAATFAIWFLTKNSKTKSLASSAGVSALLGITEPALFGVNLKYR
                     FPFFCALIGSGIATAVAGLLKVVAVSLGSAGFLGFLSINATSIPFYVLCELISFVVAF
                     AITYFYGKTKAAGIFKAEEIAEQTPVNSSKVATTDPISTDVESTQTKVSSETIVSPIS
                     GKVISLSSVNDPVFSSEAMGKGIAIKPNGNTVHSPVDGTIQLTFETGHAYGIKSNNGA
                     EVLIHIGIDTVSMGGKGFTQKVTTNQKVRKGDILGAFDRSMIEKEGLDDTTMIIVTNT
                     SDYSEVNIVAENTVTEGTDLLEIK"
     gene            4995..5867
                     /locus_tag="SCJ86_002668"
     CDS             4995..5867
                     /locus_tag="SCJ86_002668"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631531.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family protein"
                     /protein_id="HEG1170156.1"
                     /translation="MLYGAIEAGGTKFVCAIGDEEMTIKERVSFPTTTPEETMPLVID
                     FFKQYQADLAGIGIGSFGPIDIHRDSATYGYITSTPKLAWQNFDFIGTMKKEFPIPIS
                     WTTDVNAAAYGEYVFGSGKGLSSVVYYTIGTGVGGGALQEGRFIEGFSHPEMGHMLVV
                     PHPKDDFAGSCPFHGNCLEGMAAGPAIEKRLGKKGQEVAEDDPYWEIEASYIAQCAYN
                     TTLMFSPDVIIFGGGVMKQRHMLQNVHDAFAKLVNGYVETPELSKYIVTPALEDNAGT
                     LGCLALAREAVLHS"
BASE COUNT         1952 a         1053 c         1079 g         2039 t
ORIGIN      
        1 cgtaaaacaa ccgtaacagt tgtgtaaaaa tatcatttta gagcatgaaa aaaggagtgg
       61 atttctccac tccaaaaaag tctaactttt gggggtcact acaagttgac aactcctttt
      121 tgtttacaaa tcaattggat atctacaaat tgtattttct aacgaatttt atagacatta
      181 aaaaattaat ttaacgttaa atacttcctc cagaaagtat tgagattggt aaaatataat
      241 ccttatttgt cttttcgcct ttaatttttt tcagaagaat atcaacaata agacttgcaa
      301 tttcatcaat tggttgtctt attgttgtca attgtgggaa aaatttctca ataaaagagg
      361 taccatcgta tccaataatt tttaaatctt ctgggatatt aagtttaagt tgatgagcaa
      421 ttttcatagt gagaatggct gttaaatcat cagaaacaaa aataccatct ggtttgtgat
      481 ttataatagt tgactttatt tccatttcac gacggatagt agacagatta ttaggaattt
      541 tgaaaatttt tccatttgga ttttgaaaag aaaaacctag tgcacgtaat tctgtgggag
      601 aatccgtgtt atcatttcct gtaatcataa tggtattttg acaaccattt ttttgtaaag
      661 tcttagctgc aagttttccc ccttcaaagt tatctgaaga aacaatggaa acattaggag
      721 ctaaatttct atcaaaagca acaataggtg ctctcacacg ctcataatca gcaattccaa
      781 gattatggct tgatgagatt atgccatcaa cttggttggc ttcaagcatt tcaatgtatt
      841 ctctctcttt agcaggatca ttttgactat tgcaaataat cgttttatat ccatgtttaa
      901 ataactctat ttcgagatac tcaatgagtt cagagtaaaa gatattacta atattaggaa
      961 aaataagacc aataagctga ggtgactttc cttgaagtcc acgtgctaaa ttatttggtt
     1021 tataaccaag agttttcata gcttcatgaa ctttttgttt tgttttttct gataaatatc
     1081 ccttattatt aatgacgcgt gaaacggttg ttgggctaac tccagctaat tcagcgacat
     1141 cagttaattt tgctaccatt ttaatatttt aattcaaagt attttccagt aggttcgcct
     1201 gatttgattt gaataccatt ttggtgattt tcagggaata cacgactagt aaacactttt
     1261 tctcctttat taataaaaat ctcaatgatt gagttatcta caaaaatgtt agcagttgtt
     1321 tctttagtat caattgagca ttcacgaatc gtaccaaagt cagtggcata ttgaatgcct
     1381 gctttgctgc gatctaaaat aatttttcct tctttggtat ctacaatcaa gctaagtcca
     1441 tttcctttac tatcagagaa aagaagtaac tccgatttct gatttggagg aaaagttagt
     1501 tcaagctcat aagtattatt tgtttctgct tttgctgtaa attgttcaga actagcacgt
     1561 aatgatgtaa tagcttctac tggatattgg taaagtttac catcttttat tgaaagttct
     1621 tttacaagac tcattgctcc ctgataatca tacttatcag ttggataatc gatatcaggt
     1681 agtccaatcc aactgacaat cagagcacga ccatcaggag tattgaatcc ttgagtcgca
     1741 tacgcttcaa atccataatc aagattttga atttctgaag cgcccacaag ttttactttt
     1801 tctatgtcaa gcgattgaca gatcttaaaa gtattcggat agatattccc ataatttagc
     1861 tcagatttat ccaacccttg aggacaataa atgagaacag gttttttgtc tacaaatact
     1921 agatttggac actcaatcat gtattctgaa ccagtgccgc caaaatcaag gttgccaact
     1981 tcctcccatg tttccacgtt attgtcatcc gctttgtaaa gtttgataaa gcctgatttt
     2041 tctaaatttt gtgctccaac aatcgcatac aattgtcctt cataattaaa catttgtgga
     2101 tcacgaaaat gatcagttac atcagcaggt ttatgaataa gaacattctc acctttcgta
     2161 atgttgtagt ctttatccat ccatgctccg atttgaagtg tatcacgttc ccaattttca
     2221 tttcgaacgt ttcctgtata gagtaagaat agattcccct ctatttcata tgcagaaccg
     2281 gaataagcgc cgtggctatc tcgttttgtg tctggaagaa gttctgctcc tgttacatga
     2341 aaatgaacta aatcatctga ttcattgtgt acccattctt tcaacccatg agcagctcca
     2401 aatggccaat tttgataaaa aagcgtatat tttccgttaa aaaaagaaaa accattagga
     2461 tcgtttaaca atcctgtttt aggttcgata tgatagctag cacgccaagg tgatcgtttt
     2521 acattatcct taattttatc catctcatct ttagtccaat cagaataagc acgatatcgt
     2581 atttctcgag gtaagttcat attttactcc ttcaatttat ctttttacaa ctagtatggt
     2641 aaacgttttc cgttgaaaaa tcaatcattt catttaaaaa aataaactat atgacaaacg
     2701 tttgacatat ttttaagata tgctaaaata aatttgcatt tcagtgaacc gttttcacgt
     2761 aaaaataaaa aaggagtgat tcgaatgaat tacagtaaag tagcttccga cgtaatacaa
     2821 gcggtcggaa aagataacct tattgcagct gcacactgcg ctacacgtct tcgtctcgta
     2881 ctaaaagatg acacgaaagt gaatcaaaaa gctttagacg aaaatccaga tgtgaaaagc
     2941 acatttaaaa ttaatggaca atatcaagtg attatcggtg caggtgatgt taatttcgtt
     3001 tatgacgaac ttatcaaaaa aactggactt tcggaaattt caacagatga tttaaaacaa
     3061 attgctagta aagataaaaa attcaacccc attatggcat taattaaatt attatcagat
     3121 atttttgttc caattatacc tgcccttgtt gcaggtggtt tattaatggc attacaaaac
     3181 tttttaacag ctgctggact ttttggacca aaatcaattg aagaaatgta tcctgcgatt
     3241 gaaggtattt cctcaatgat ccaactgatg tccgctgcac cattcatgtt cttaccaatt
     3301 ttagtaggta tttcagctgc taaacgtttt ggagcaaacc aatttcttgg tgctgcaatt
     3361 ggtatgatca tgacaacacc agatcttggt ggctcaactg aatattggaa tatttttggt
     3421 tatcatgttt cacaaacaaa ctatgcttat caagttattc cagttttagc agctgtttgg
     3481 atattatcta tattagaaaa attctttcat aaaaaattgc catcatctat tgactttaca
     3541 ttcacgccat tattatcagt aatgattact ggatttttga ctttcacagt tattggccct
     3601 gtaatgttat tgttgtctaa tggaattaca gacattattg tttggctata taacactact
     3661 ggatttattg gaatgggtat ttttggagga acatattcac ttatagtcat gactggttta
     3721 catcaatcat ttccagctat tgaaactcaa ttactttcag cgtggacaaa tggtatcgga
     3781 catggagact ttatctttgt agttgcatct atggcaaacg ttgctcaagg tgctgcaaca
     3841 tttgccattt ggttcttaac taaaaattct aagacaaaaa gtcttgcttc ctctgcgggt
     3901 gtctcagcac ttcttggaat tacagagcct gccttgttcg gagttaactt aaaataccgc
     3961 tttccattct tttgtgcttt aattggttca ggaattgcta cagcagtagc aggattactt
     4021 aaagtagttg ctgtttcact tggttcagca ggtttcttgg gatttctttc aattaatgca
     4081 acatctattc cattttacgt attgtgtgaa ttgattagct ttgtagttgc ttttgcaatt
     4141 acttacttct acggtaaaac aaaagctgct ggtatcttta aagcagaaga aattgctgaa
     4201 caaacaccag ttaattcttc aaaagtagca acaacagatc caatatctac tgatgttgaa
     4261 tcaacacaaa caaaagtttc atcagaaaca attgttagtc ccatttctgg taaagtgatt
     4321 agtctttctt cagtaaatga tccagtcttt tcatcagaag ctatgggaaa agggattgcc
     4381 attaaaccaa atggaaacac ggtccattct ccagtagatg gtactattca actcacgttt
     4441 gaaactggac atgcttatgg tattaaatca aataatggtg ctgaagttct aattcatatt
     4501 ggtattgata ctgtatcaat gggaggaaaa ggatttacac aaaaagttac tactaatcaa
     4561 aaagttagaa aaggcgatat tcttggagca tttgaccgtt caatgattga gaaagaaggt
     4621 cttgatgata caacaatgat tattgtgacc aatacttcgg attattcaga agtaaatata
     4681 gtagctgaaa atacagtcac tgagggtact gatttacttg aaatcaaata aatatatttc
     4741 cctgagtaaa gatggaattt tctttccaaa atcaaataat atagattaat caaatatttg
     4801 agaataacat tttatttaat cttttcatat atcaattgta tgaaaaatta ttagaatttt
     4861 aaaaaatgat aaaaattcaa aaagaaaaaa tagtagttat ttacttgtca tgttactaaa
     4921 taaaaaaata atttaaatat gatatatagt catttattta gtgatttcat tctaaaataa
     4981 agggagaaat gtctatgcta tacggtgcaa ttgaagcagg cggcacaaaa tttgtgtgtg
     5041 ccattggcga tgaagaaatg acgatcaagg aaagagtgag ctttccgaca acgacacctg
     5101 aggaaacaat gcctttagtc attgacttct tcaaacaata ccaagcagat ttagcaggga
     5161 ttgggattgg ctcatttggg cctatcgata tccatcgcga ttccgccact tatgggtaca
     5221 ttacctcgac cccgaaattg gcatggcaaa attttgattt catcggtacc atgaaaaaag
     5281 aatttccgat cccgatttct tggacaacag atgtcaatgc ggctgcctat ggtgagtatg
     5341 tcttcggcag tggcaaagga ttgagcagtg tcgtttacta tacgatcggt acgggtgttg
     5401 gcggcggtgc cttacaagaa gggcgcttta tcgaagggtt tagtcatcca gaaatggggc
     5461 atatgctcgt cgtccctcat ccaaaagatg actttgctgg cagctgtcca tttcatggca
     5521 actgtttaga agggatggcc gctggacccg ccatcgaaaa acgcttaggg aaaaaaggac
     5581 aagaggttgc agaagatgat ccttattggg aaatcgaagc ctcctatatt gctcaatgtg
     5641 cctacaatac aaccttgatg ttttctccag atgtgatcat ttttggcggt ggtgtgatga
     5701 aacaacgcca tatgctgcaa aacgtccacg acgcatttgc aaaattggtc aatgggtatg
     5761 tcgaaacacc tgagttaagc aaatatatcg tgaccccagc attagaagac aacgctggaa
     5821 cgctcggttg tttggcattg gctcgagaag cggtcttaca ttcataaacg aaaaaaggct
     5881 cttcgacatt tagtgttggt agagaaagat acaactgaaa atttagtcat aaccagttag
     5941 gcagagtgct taactggttt ttgttttact tggaagatca agccttgaat gagataaatt
     6001 cttgctcgaa agttatggaa atttcgatag ccgtaggcca cttgtattgt gcaatagttt
     6061 tttacacgcc gtttgtataa ttaagcgaga caaataaaaa agccatttat gttacactca
     6121 aat
//
LOCUS       DARBET010000115         6001 bp    DNA     linear   BCT 07-NOV-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4027
            SAMEA114569841-rid20112183.denovo.119, whole genome shotgun
            sequence.
ACCESSION   DARBET010000115 DARBET010000000
VERSION     DARBET010000115.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA114569841
            Sequence Read Archive: ERR12203666
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 6001)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6001)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001972618.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001972618.1
            Genome Coverage       :: 132x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/07/2023 13:18:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,041
            CDSs (total)                      :: 2,975
            Genes (coding)                    :: 2,908
            CDSs (with protein)               :: 2,908
            Genes (RNA)                       :: 66
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 67
            CDSs (without protein)            :: 67
            Pseudo Genes (ambiguous residues) :: 0 of 67
            Pseudo Genes (frameshifted)       :: 26 of 67
            Pseudo Genes (incomplete)         :: 28 of 67
            Pseudo Genes (internal stop)      :: 20 of 67
            Pseudo Genes (multiple problems)  :: 6 of 67
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6001
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA114569841-rid20112183.denovo.119"
                     /isolate="VRE4027"
                     /db_xref="taxon:1352"
                     /country="Denmark"
     gene            <1..395
                     /locus_tag="SCJ86_002669"
     CDS             <1..395
                     /locus_tag="SCJ86_002669"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347917.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEG1170157.1"
                     /translation="ISLKDESNTTVSDANVLANIILDFIFEHHIPFKEGYEILPANQE
                     YYFYKCITKRVCCICGKTGADIDHFDKALGRRKRKEVDHSEYTFAALCRIHHTEKHKI
                     GVINFKNKYQIKGIKLSHETIKKLRIGG"
     gene            398..1228
                     /locus_tag="SCJ86_002670"
     CDS             398..1228
                     /locus_tag="SCJ86_002670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336322.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage replisome organizer N-terminal
                     domain-containing protein"
                     /protein_id="HEG1170158.1"
                     /translation="MAEISWIKLKTTMFDDEKIRLIQAVPESDAIIVIWIRLLVLAGK
                     TNDDGLIYIQRNMPYTEEMLATLFGKNVNTVRLALTTLANFNMIDLSSDGLIAISNWE
                     KHQNIEGMDKVRLKNAERNRKYRERKRQERLKLENDVSVTSRDGTDKDIEEDKDIDKE
                     EKKGKYSDEHLRLAKKMQRNLTEDFPKEMNKVDIEKWADTIRLMEERDKASIEAIEYV
                     INWLPTNEFWFGNIRSAKKLREKFEKLKFEIKADKKNHKKQSQKLQYSNPSEYDDLPI
                     "
     gene            1244..2095
                     /locus_tag="SCJ86_002671"
     CDS             1244..2095
                     /locus_tag="SCJ86_002671"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305396.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HEG1170159.1"
                     /translation="MESLANAMEKLIRRVLVQSGKCPECSEPLYSWRAKNKDGSERCK
                     PTCMSCGYKALRVKEDIQTERIYNDSLKARALSFFQNGSVLTDKTLFKCKMENYHVVD
                     QETKIALERAKSYVNDVLLNHPAHFILSGKSGSGKSHLSMATAWEILERSNYDKKILF
                     ISYQELLEQIKFSYNNAELRKEIEGSLIADIKTTDLVVFDDIGAELGSGVSNSRQFTN
                     NTLNTLLEARQNKATIITTNLSGPELREAYGERIVSRIFKNSEGYALKFQQTADKRIK
                     PVKGSIA"
     gene            2092..2451
                     /locus_tag="SCJ86_002672"
     CDS             2092..2451
                     /locus_tag="SCJ86_002672"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304469.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1064 domain-containing protein"
                     /protein_id="HEG1170160.1"
                     /translation="MNKYRNKKTVHRGIKFDSIAEAEYYDLALWQAEANGWKVKLQER
                     FELMPKFELDGKKYRKIEYIPDFTFYKNGKLVKVVDVKGMQTKDFKIKAKLFCSQYRV
                     PLILAKKYRNTFKEERF"
     gene            2466..2627
                     /locus_tag="SCJ86_002673"
                     /pseudo
     CDS             2466..2627
                     /locus_tag="SCJ86_002673"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010752308.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="antitoxin"
     gene            2624..2929
                     /locus_tag="SCJ86_002674"
     CDS             2624..2929
                     /locus_tag="SCJ86_002674"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628584.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEG1170161.1"
                     /translation="MSVDKKAAMKRIIELTHSENWQEDKEIVAEVQKLGKSMWAEKPK
                     RKTPRKIAIWHGDRILVTGTAEQLSEITGLSKNIIWDRARSLWIDSKGRQFRYVEEK"
     gene            2929..3240
                     /locus_tag="SCJ86_002675"
     CDS             2929..3240
                     /locus_tag="SCJ86_002675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341726.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MazG-like family protein"
                     /protein_id="HEG1170162.1"
                     /translation="MNELITKVEQWAKDKGLDQADPKAQFLKVAEEFGEIASAMARSN
                     DELFKDSVGDVIVTLIILSMQKGANIQECLEMAYNEIKGRTGKMVDGVFVKSSDLEDS
                     E"
     gene            3237..3410
                     /locus_tag="SCJ86_002676"
     CDS             3237..3410
                     /locus_tag="SCJ86_002676"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375499.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEG1170163.1"
                     /translation="MMMPKEIYSDALRGARNQLKMAKRAYEIRPTIENERRVKAIRRR
                     CSIYGELQKEDSK"
     gene            3407..3823
                     /locus_tag="SCJ86_002677"
     CDS             3407..3823
                     /locus_tag="SCJ86_002677"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002352294.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YopX family protein"
                     /protein_id="HEG1170164.1"
                     /translation="MIPRFRAWDKRKNVMRDVAVLHFTKNGKTNFIEYWINPTELKSY
                     HVRNIELMQSTGLKDKNGIEIFEWDIVSVSVRNGFDYLDNKVCVVKNSIGHSGLVCAT
                     VDEDLEYQIFNTELFEEYTYEVIGNIWENSELLEEQ"
     gene            3823..4491
                     /locus_tag="SCJ86_002678"
     CDS             3823..4491
                     /locus_tag="SCJ86_002678"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333290.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1642 domain-containing protein"
                     /protein_id="HEG1170165.1"
                     /translation="MNKQELIDELAKYVKRYENVMDEHGQGRYGAYEVSLKLVKRLNE
                     SKITDEQAWNKVAEAYPESAQSLRNTLDNAVFGKTGEPQKHVMPKFVAEWIEYAKKKG
                     DSLAISFKPWNLYGVEYSKADRWIGDNQETFARAWLDGYEVEKEPLYYVPLPYEVWDE
                     EAAELKTEYLYLHYEITSDETRIFPTKEPRKGFVAKLDELTIKSADENYWPFAVPVEE
                     VVEG"
     gene            4488..4865
                     /locus_tag="SCJ86_002679"
     CDS             4488..4865
                     /locus_tag="SCJ86_002679"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002351704.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEG1170166.1"
                     /translation="MKKLHQLISEKESELQNLEDSLGLGFPIVEQVKMVQISHLQLEL
                     EDLRQIEDPYQLNDNQQIVLEWLKLTASTGKPMQVVFWMMNNAAWGHLDELRDPLMEL
                     TDKQQFEVLTAFAQWGLEQEEKE"
     gene            4862..5161
                     /locus_tag="SCJ86_002680"
     CDS             4862..5161
                     /locus_tag="SCJ86_002680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315373.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEG1170167.1"
                     /translation="MKLKDGFYASSHGIGGLMLDMPTKNPKTRKKPKVKVGDMVRCEA
                     EGFIYPFRGYVEHVYNHSAIIRIENTMECDKLLAKSKDNLAVVRLVDMEVINNEI"
     gene            5151..5309
                     /locus_tag="SCJ86_002681"
     CDS             5151..5309
                     /locus_tag="SCJ86_002681"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347924.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEG1170168.1"
                     /translation="MKFKIFEEDTRYKLEKKLNEFAKNNEIQHISLTASKSGYTTYYA
                     AVVSYVIQ"
     gene            5403..5819
                     /locus_tag="SCJ86_002682"
     CDS             5403..5819
                     /locus_tag="SCJ86_002682"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016252615.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="autolysin"
                     /protein_id="HEG1170169.1"
                     /translation="MQLLREVDFKQTRCNARDVLKNFRRLERMAGRSLIDIKSPIITD
                     MPKAPKHGNKAEDAIIQMMDIEAERDAILAALMALSLISRQILYYSFCDVNKHSNYEI
                     GQLIRGYGEKNVEKLKSIALIEFAEAYKKGVLVQYR"
BASE COUNT         2230 a          899 c         1391 g         1481 t
ORIGIN      
        1 aaataagcct caaagatgaa tcgaatacga ctgtgagtga tgctaatgtc ttagcaaata
       61 tcatcttaga tttcatcttt gaacatcata ttcctttcaa agaaggctat gagattttac
      121 cagcgaatca agaatattac ttctacaaat gcattacaaa aagagtttgt tgcatctgtg
      181 gcaaaacagg agctgacatc gatcactttg acaaagcgct aggaagacga aagcgaaaag
      241 aagttgatca ttcagagtac acatttgcag cactctgcag aatccatcac acggagaagc
      301 acaaaatagg tgtgatcaat tttaaaaata aatatcaaat caaaggaatc aagttaagtc
      361 atgaaacgat taaaaagtta aggataggag gataaatttg gctgagataa gttggatcaa
      421 acttaaaact actatgtttg acgatgaaaa aatacggtta atccaagctg ttcctgagtc
      481 ggatgccatc atcgttatat ggattcgatt actagtttta gcaggaaaga ctaacgacga
      541 tggtctgata tatatccaga ggaacatgcc ttataccgaa gaaatgcttg ctacattgtt
      601 tggcaaaaac gtaaatacgg ttcgcttagc gctaactaca ttggcaaatt tcaacatgat
      661 tgatctaagc agtgatggac taattgccat cagtaattgg gaaaaacatc aaaatatcga
      721 gggtatggat aaagtaaggc taaaaaatgc tgaaagaaac cgtaaataca gggaaagaaa
      781 gagacaggaa cgtctcaaat tggaaaatga cgttagcgtg acgtcacgtg acggtacaga
      841 taaagatata gaagaagata aagatataga taaagaagaa aagaaaggta agtattctga
      901 cgaacactta cgccttgcta aaaaaatgca aaggaattta actgaagatt ttccaaaaga
      961 aatgaacaaa gtagatatcg aaaaatgggc agacacaatc aggttgatgg aagaaagaga
     1021 taaagcatct atagaagcga ttgagtatgt gatcaattgg ctacctacaa atgaattttg
     1081 gtttggaaat attagaagtg ctaagaaatt gagagaaaaa tttgagaagc tcaaattcga
     1141 aatcaaagca gacaagaaga atcataaaaa gcaaagtcaa aaactacagt acagcaatcc
     1201 tagtgaatat gacgacttgc caatttaaaa ggagatgcat cacatggaaa gcctagcaaa
     1261 tgctatggag aagctaataa gaagagtatt agtgcaaagc ggaaaatgtc cagaatgtag
     1321 cgaacctttg tatagttggc gagctaaaaa taaggatggt tcagaacgtt gtaaaccaac
     1381 atgtatgagt tgtggttata aagcgttacg tgtgaaagag gatatacaga ccgaacggat
     1441 atataacgac agcttaaaag cacgagcatt gagttttttt caaaatggtt cggtattaac
     1501 agataaaact ttgtttaaat gcaaaatgga gaattatcac gtagtggacc aagaaacgaa
     1561 aattgcttta gaaagagcta aaagctatgt aaatgatgtc ctactgaacc atcctgcaca
     1621 tttcattcta tcagggaaat caggaagcgg aaaaagccac ttatcaatgg cgacagcttg
     1681 ggaaatactt gagcgctcaa attatgacaa gaaaatactt tttataagct atcaagagtt
     1741 attagagcaa ataaagtttt cttataacaa tgctgaactg agaaaagaaa ttgaaggatc
     1801 gcttatagcc gatatcaaaa caactgattt ggtggttttt gacgatattg gagctgaatt
     1861 aggtagcggg gtatcaaata gtaggcaatt tacaaacaac acgttaaaca cgctcttaga
     1921 agccagacag aacaaggcaa cgatcatcac aacaaactta tctggtcctg aactaagaga
     1981 agcctacggt gaaagaattg tttctaggat atttaagaat tcagaaggtt atgcgctgaa
     2041 attccaacaa acagcagaca agcgcataaa accagtgaaa ggtagtatcg catgaataaa
     2101 taccgtaata aaaaaactgt tcatcgaggt atcaagtttg attctatcgc agaagcagag
     2161 tattacgatc tagccttgtg gcaagctgaa gcgaacggct ggaaagtaaa acttcaggaa
     2221 agatttgagc tgatgccgaa atttgaacta gacggaaaga agtatcgcaa gatcgagtat
     2281 attcccgact tcacatttta taaaaacggc aaactagtca aagtcgtaga tgttaaaggg
     2341 atgcagacaa aagatttcaa aatcaaagcg aaactcttct gcagtcaata tcgagtacca
     2401 ctgatcttag cgaagaaata tcggaatacg ttcaaggaag aacggtttta acgaggtgat
     2461 ccatcatgac aacagaagaa gtgattcaaa tgcgtattcg aagccttcag cgtgagattg
     2521 acgatctgta acggacaaag gcagtgatgg tcaatgaaac ggcgaggaag gcaatcgatt
     2581 tgcacataga aaatttaaga aggaaaatcc atcgattgga ggaatgagcg tggataagaa
     2641 agcagcaatg aaacgaatca tcgaactgac acattctgag aattggcaag aagacaaaga
     2701 aatagttgca gaagtccaaa agctcggtaa atcaatgtgg gctgaaaagc ctaaacggaa
     2761 aacgccgaga aaaattgcaa tctggcatgg tgatcgaatt ctagtaacag gtactgctga
     2821 acagttatct gaaattactg gattaagcaa aaacattatc tgggatagag ctaggagctt
     2881 atggattgat tcaaaaggac gacagtttag gtatgtggag gagaaataat gaacgaacta
     2941 atcacaaaag tagagcagtg ggctaaagac aagggactgg atcaagcgga tccaaaagca
     3001 caatttttga aagtagctga ggaattcgga gaaattgcat cggcaatggc aagaagtaat
     3061 gatgagctat ttaaagatag cgtaggagac gttatcgtca cgctgattat tctttccatg
     3121 caaaaagggg caaacataca agagtgttta gaaatggcgt ataacgaaat caaaggacgc
     3181 acaggaaaaa tggtagatgg tgtattcgtg aagtcgagtg atttggagga cagcgaatga
     3241 tgatgccaaa agaaatctat tcagatgcat taagaggtgc tagaaatcaa ttgaaaatgg
     3301 caaaaagagc atacgaaatt cggcccacaa tcgaaaatga acgcagagta aaagcaattc
     3361 gccgtagatg ttcaatttac ggcgagttac agaaggagga cagcaaatga taccgagatt
     3421 tcgagcttgg gataaacgaa agaacgtaat gagagatgta gccgtcttgc attttactaa
     3481 aaacggcaaa acaaacttta ttgaatattg gataaatcct accgaattga aatcatatca
     3541 cgtgcgaaac atcgagctca tgcaatcaac agggctgaaa gataagaatg gcattgagat
     3601 tttcgagtgg gatattgtat cagtcagcgt gcgaaatgga ttcgattatt tagataataa
     3661 agtttgtgtt gtcaaaaatt caataggaca ttcgggatta gtgtgtgcca ctgttgatga
     3721 agatttagag tatcaaattt ttaacacaga gctgtttgaa gaatacacgt atgaagtcat
     3781 cggaaatatt tgggagaata gcgagttatt ggaggaacag tgatgaataa acaggaattg
     3841 attgatgaat tagctaaata tgtaaagcgt tatgagaacg ttatggatga gcatggccaa
     3901 ggaaggtacg gcgcttatga agtatcttta aagttggtga aaagactaaa tgaatcaaaa
     3961 attacggacg aacaagcttg gaataaggta gctgaggctt atcctgaatc ggcacaaagc
     4021 ttgagaaaca ctttagataa tgctgtattt ggtaagactg gtgaaccgca gaagcacgtt
     4081 atgccgaagt ttgtggcgga gtggatagaa tacgctaaaa agaaaggtga tagtctagct
     4141 atttcattca agccgtggaa cctctatggt gttgagtata gcaaggctga tagatggatt
     4201 ggagataatc aagaaacgtt tgctcgtgct tggctagacg gctacgaggt cgagaaagag
     4261 ccgttgtatt atgtcccgct accatatgaa gtttgggatg aagaggcagc ggagcttaaa
     4321 acagagtatt tgtatctaca ttatgagatc acaagtgatg aaactagaat atttcctaca
     4381 aaagaaccta gaaaaggttt tgtcgcaaaa ttagatgaac ttacgattaa atcagcagat
     4441 gaaaactact ggccgtttgc tgtaccagtg gaagaggtgg tagaaggatg aaaaaattgc
     4501 accaactcat atctgagaaa gaaagcgaac tccaaaattt agaagatagt ttgggattgg
     4561 ggtttccaat tgttgagcaa gttaaaatgg ttcaaatcag tcatctacaa cttgaattgg
     4621 aagatttaag acaaatagaa gatccgtatc aactcaacga taaccagcaa atcgtgttgg
     4681 agtggttgaa actaactgcg tcaacaggaa agccaatgca agttgtattt tggatgatga
     4741 ataatgcagc atgggggcat ttagacgaat tgagggatcc tttgatggaa ttaactgaca
     4801 aacagcaatt cgaagtccta acagcattcg ctcaatgggg attagaacag gaggaaaaag
     4861 aatgaaacta aaagacggat tttacgctag tagccacggt atcggcggtt taatgctaga
     4921 catgccgaca aagaacccta aaacacgtaa gaagccaaaa gtcaaagtcg gtgacatggt
     4981 tcgctgtgaa gcagaaggat tcatctatcc atttcgtgga tatgtagaac acgtctataa
     5041 tcactcagcg atcattcgca ttgaaaacac gatggaatgc gacaagttat tagcgaaaag
     5101 caaagataat ttagcagtgg tgagattggt ggatatggag gttataaaca atgaaattta
     5161 aaatctttga agaggacact cgctataaat tagaaaaaaa attaaatgaa tttgcaaaga
     5221 ataatgaaat tcagcatata tctttaacgg cttctaagtc aggttataca acttactatg
     5281 cagctgttgt gagctacgta attcaataaa aggaactcgg caaataaaaa agccggatca
     5341 ctccgactga tgtaataaat ccgacaagtt tattatatca cataaaggag cggtttgact
     5401 tgatgcaatt gttacgagag gtagatttca aacagacaag atgtaatgcg agagatgtgc
     5461 tgaagaactt tcggcgcttg gagcggatgg caggtcgctc tttgatagat attaagtcgc
     5521 cgattattac ggatatgccg aaggcaccga agcacggcaa taaggcagaa gacgcgatca
     5581 ttcagatgat ggatatagaa gcggagagag acgcgatttt agcggctttg atggcgctta
     5641 gtctgattag ccgtcagata ctctactaca gcttctgtga cgtaaacaag cactctaatt
     5701 atgaaatagg gcaattgata cgaggatacg gagagaagaa tgtagagaag ctgaaatcca
     5761 tcgcattgat cgaatttgct gaagcataca aaaaaggcgt gttagttcag tatcgttaat
     5821 tttgtagggt ttttgtaggg atagtgtagg gtttttgagc ggtttaacgt gatattatga
     5881 tagtgtcgaa agattagtga taggtctaag acaaaataat aataaaagga acatcgtttt
     5941 attattgttt cacaattaag cttcgataga cagcagcgga aatattaaga ataaggatgt
     6001 g
//
LOCUS       DARBET010000116         5809 bp    DNA     linear   BCT 07-NOV-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4027
            SAMEA114569841-rid20112183.denovo.120, whole genome shotgun
            sequence.
ACCESSION   DARBET010000116 DARBET010000000
VERSION     DARBET010000116.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA114569841
            Sequence Read Archive: ERR12203666
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 5809)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5809)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001972618.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001972618.1
            Genome Coverage       :: 132x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/07/2023 13:18:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,041
            CDSs (total)                      :: 2,975
            Genes (coding)                    :: 2,908
            CDSs (with protein)               :: 2,908
            Genes (RNA)                       :: 66
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 67
            CDSs (without protein)            :: 67
            Pseudo Genes (ambiguous residues) :: 0 of 67
            Pseudo Genes (frameshifted)       :: 26 of 67
            Pseudo Genes (incomplete)         :: 28 of 67
            Pseudo Genes (internal stop)      :: 20 of 67
            Pseudo Genes (multiple problems)  :: 6 of 67
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5809
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA114569841-rid20112183.denovo.120"
                     /isolate="VRE4027"
                     /db_xref="taxon:1352"
                     /country="Denmark"
     gene            450..1604
                     /locus_tag="SCJ86_002683"
     CDS             450..1604
                     /locus_tag="SCJ86_002683"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348970.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase C47"
                     /protein_id="HEG1170170.1"
                     /translation="MIILKPKNKIYLGLLFSLMIGIFSIGITNTYASENSNFNINSQP
                     IEEQAINFAKTDFEHSITSILLMENNTETDINYTLGSPFKMLDGENTANSSIFFPVFS
                     DADNTIKYVYTVKKNTNGEYSGSISQFLAKELQNLVDSNITDTTLFFQDNSIYYSDNG
                     QIKLLWKSPLSTEKNGVSSFTEKNDLISVNIKNKIKNFSKKQNFPAVKRVKSVSSGDN
                     FIMINWRIAETQTSFPWCAAYVEAAILCNKTDVRATSARAIMHYTYPNLNNSQIESKS
                     LNQDQIIKYANYCGVYPKKVARILNLSEVQTQLRKGNAIYMGTAGYGNYSNSRHALAL
                     FGWVNQGGYQTYYVWNPWYNYPTVISANAGPVTIPVPGGGFTWDNTITNW"
     gene            1598..2020
                     /locus_tag="SCJ86_002684"
     CDS             1598..2020
                     /locus_tag="SCJ86_002684"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEG1170171.1"
                     /translation="MVNKPIVLVMGLVLVLFLTSCSKKQEIQKTELIPFLGTWQTKDH
                     SERLVITNLNTSKNIEQYTILVKENNHKIQIIDKNLNINEQVFIYKATNNLLTLRSNK
                     KQLDYTFSLDSPKKLVFILTTNDGTEGTAKPIEFFKAD"
     gene            3143..4075
                     /locus_tag="SCJ86_002685"
     CDS             3143..4075
                     /locus_tag="SCJ86_002685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296860.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation protein"
                     /protein_id="HEG1170172.1"
                     /translation="MANEIVKYKPELNTIPLRKFTPIEMNLFFSIVSRMRDKGNQKIR
                     FYFDQLKDLSAYKQTAGEVFIKDLTRTYDHLMDLRFGKKSNGGLTIERFTMFTDFEIH
                     GEADNPYVDIKVHEKALPLLNNLESWVRFSLTEFRDLNSSYAKTMFRLLKQYRTQGWA
                     EFSKEDFLELLDIPKSYRQTNINQFVLKPIKEELTPLFKGLTIRKKYGKGRGKPVIGY
                     RFTWKAEINHADDFSKGKQEDLRTKLFNIEHNGELTQEEKWRAKDRILDLPLGTHEAN
                     FKKQQQTEKEEVEKQTLSNELKQDLLGNLQNLFD"
     gene            4092..4607
                     /locus_tag="SCJ86_002686"
     CDS             4092..4607
                     /locus_tag="SCJ86_002686"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305828.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF536 domain-containing protein"
                     /protein_id="HEG1170173.1"
                     /translation="MADKTIKDLAEELGVSKTAINKKVSDENRKLWFAKIGNKFVINE
                     IGQKAIKSMFFTENENSNQQPVGEKSKTENHKNANQVSELELFFFLEKQLNQKDIQLQ
                     EKDEQLKQMQKLLDQQQQLTLQANQQIKKLHEQLQLTYAEESSEKEPLSVTEETQKGQ
                     NKEKKWWQFWQ"
     gene            4903..5184
                     /locus_tag="SCJ86_002687"
     CDS             4903..5184
                     /locus_tag="SCJ86_002687"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEG1170174.1"
                     /translation="MDILVRNINPKIITRVDKRCQELTTKTGEKWSRNKYLKVLIESD
                     FDRQLMNYKKEQFDLLLEKFIDIQTKNTETLNDYVHTNNQLIETLIGGI"
     gene            5186..5602
                     /locus_tag="SCJ86_002688"
     CDS             5186..5602
                     /locus_tag="SCJ86_002688"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001232859.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEG1170175.1"
                     /translation="MRKTSVQKHVTLYPSHIDVLDSIIEKNPDINNYSEAIRFLCMSY
                     EPDTNTTTTSNQAKLNAMGKEISIMTEIIAEFADLYLKDTGVLIGTNSKVYQEVKEKI
                     EKQLNPSQRNQPKNRQKETSQTFTSKNFDPTNFLKR"
BASE COUNT         2228 a          961 c          930 g         1690 t
ORIGIN      
        1 aaaaaaaaag actgctcgaa agagcagtct caagcaaaaa ataaacgaac aaacgcaatt
       61 actcacgaac acaaacaaat acaataaaat acctaaataa tgaaaataag tgataaaaaa
      121 caagggtatt tatagtttaa caagcgctca taaaaataac aagtctttaa aaaatgacag
      181 ataaaaatgc acaaagaatc ggacacagat aaccgcgaaa aatcaagctt tagaaagttg
      241 tttttagcta gaccaatgaa aaatcacaaa gtccgcccct gtatccgttg gtagaggggc
      301 tacttggttt agtatacatt atgttaacta gctcgttaaa taacgattat gttcccaaaa
      361 caatatttta tgaactataa caaaaatttg ttatcgcata ttgtatattt taattatagg
      421 tgtatatcac ttaattttaa cacaaaggcg tgattatttt gaaacccaaa aataaaattt
      481 atctaggtct cctcttttcc ttgatgattg gaatttttag tataggaatt acaaatacct
      541 atgcttcaga aaattctaat ttcaatatca actcccaacc aattgaagaa caagcaatta
      601 attttgcgaa aactgatttt gaacactcca taacttcaat tcttttaatg gaaaataaca
      661 ctgaaactga tattaactat actcttggtt cgccatttaa aatgctcgat ggagaaaata
      721 cggcaaatag ttctattttc tttccagtat tttcagacgc tgataatacg attaaatatg
      781 tttataccgt caaaaaaaat acaaatggag aatattcagg aagtatttca caatttttag
      841 caaaagaact tcaaaatcta gtagatagca atataacaga tacaacctta ttttttcaag
      901 ataatagcat ttattattcc gataatggcc aaattaaact actttggaaa tcacctttaa
      961 gtacagaaaa aaatggtgtc tcttccttta cagaaaaaaa tgatcttata tccgtaaata
     1021 taaaaaataa aattaaaaat ttctctaaaa aacaaaattt tcctgcggtt aaacgtgtaa
     1081 aaagtgtctc ctccggagat aactttatta tgattaattg gcgtatagca gaaacccaaa
     1141 caagttttcc gtggtgcgct gcttacgtag aagctgctat cttatgtaat aaaacagatg
     1201 tacgcgctac ttcagctaga gccattatgc attataccta tcctaattta aacaacagtc
     1261 aaattgaatc aaaaagtctt aatcaagatc aaataataaa atatgcaaat tactgtggtg
     1321 tttatcctaa aaaagtagca cgaatactaa acctttcaga ggtccaaaca caactaagaa
     1381 aaggaaatgc catttatatg ggaactgctg gttatggaaa ttatagtaac agccgtcatg
     1441 cacttgcatt atttgggtgg gtcaatcaag gtggttatca aacttactat gtgtggaatc
     1501 cttggtataa ctacccaact gtaataagtg caaatgcagg gccagttaca attcctgtac
     1561 ctggaggtgg ttttacttgg gacaatacca ttacaaattg gtaaataaac ccattgtttt
     1621 agttatgggt ctagtgttag ttcttttctt aactagttgt tcaaaaaaac aagaaataca
     1681 aaagactgag ctgattcctt ttctaggaac ttggcaaact aaagatcact cagaacgtct
     1741 agtaatcact aatttaaata cttcaaaaaa tatagaacaa tataccatct tagtaaaaga
     1801 gaacaatcat aaaattcaaa tcattgataa aaatttaaat ataaatgaac aagttttcat
     1861 ttacaaagca acaaataatt tactaacact gcgctctaat aaaaaacaat tagattacac
     1921 ttttagtttg gattctccaa aaaagctcgt atttattttg acaacgaatg atgggacaga
     1981 agggacagct aaaccaattg aattttttaa agctgattga tgaatagttg tttgatatta
     2041 caaaacaata atttcaccga aggtgaaaca agcgaaactt tacaaaaagt ggctagcatt
     2101 tgctagcctt ttttgtattg cggagcgtga gttttgaggg ggattcaagg gggagtttct
     2161 cccctttgac aagcggttat ttgcaccaaa tacgaaggta tttggctagc aaatatagag
     2221 cttgccaaac cctttagtgt gttttgtaga aagggacctt atagtcactt tcacattaca
     2281 aacgatacgt cctatggacg tatcaaaagg gtgggactta tgagcaaatt aaagcaaaat
     2341 ttggctagca atccttctaa aaaatacacc tattgatcag agtcaaaata aatcagtttt
     2401 cgagtgagcg aatccgaatt tgcaaatctg aagctttgca aatgagtgtg ccggcttccg
     2461 taaaagtcta ggtaacatta gtcgatatat acataaggct ctaatttgcg ttttaaggcg
     2521 ttttaaacgt tctggtatgt aatttatacc aaagcgactt taaaacgcct taaaacgaat
     2581 tttataggtc ttgtaggacg tttcagggtt gttagggctt gccctgaccg cctgtaaggc
     2641 gctttaaaaa caagataagg gattttatct agacttgtct taaaacgccg tagatgagcg
     2701 ctcagagctt cttaaaggac tccttacgaa ctgtttttaa gcaaaggagc ctttaggcga
     2761 ctgcgcagtt caatgtgaga gcgtagcgat cacaccttta aagcgaccgt agggagcaga
     2821 ttcccagttt aggaaatttc gcgaacctgt ttggagaaaa aaagtaggaa atttttaggc
     2881 gggaaatttt gcccccctct tttataatct cttttataac ctctttttaa acctctttac
     2941 cgtcggctgt atccttactc tcccaaggcc tgtagctttt ttagggcatg aaattatctg
     3001 tttataggca tgaaattatc tgtttatagg catgaaatta tctgtttata ggcatgaaat
     3061 tatctgtttt aggtattgtt ttaagacata atatacctta aaattatgtc aataaaatta
     3121 gttagactgg agtatcaaat aaatggcaaa tgaaatcgta aaatataaac cagaattaaa
     3181 taccattcct ttaaggaaat ttactccaat agaaatgaac ttgttttttt ctattgtttc
     3241 tagaatgcgt gataaaggta atcaaaaaat acgcttttat ttcgatcagc ttaaagattt
     3301 aagtgcttat aagcaaactg ctggagaagt ctttataaaa gatttaacaa gaacatacga
     3361 tcatttaatg gatttacgtt ttggaaaaaa aagcaatggt ggattaacca tagaacgatt
     3421 tacaatgttt actgattttg aaattcatgg agaagccgat aacccttatg tggatataaa
     3481 agttcacgaa aaagctttgc ctttacttaa caatttagaa agttgggtac gtttctcttt
     3541 aacagaattt cgagatttaa atagcagtta tgctaaaaca atgtttagat tactaaaaca
     3601 ataccgaaca caaggatggg ctgaattttc caaggaagat tttttagaac tattagatat
     3661 tcctaaaagc tatagacaaa caaatataaa tcagtttgta ctcaaaccca taaaagaaga
     3721 actcacacca ttatttaaag gattaacgat tcgtaaaaaa tatggtaaag gccgaggcaa
     3781 acctgttatt ggttatcgtt ttacttggaa agctgaaatt aatcatgctg acgatttttc
     3841 aaaagggaag caagaagatt tacgtacaaa attatttaat attgaacaca atggagaatt
     3901 aacgcaagaa gaaaaatggc gcgctaaaga tcgaatacta gatcttccgt taggtactca
     3961 tgaagctaat tttaaaaaac aacaacaaac tgaaaaagaa gaagtggaaa aacaaacact
     4021 ttcaaacgaa cttaaacaag atttattagg aaatttacaa aacttattcg attaaagtat
     4081 aaggagtatt tatggcagat aaaacgatca aagaccttgc tgaagaatta ggagttagta
     4141 agacagctat taacaaaaaa gtttctgacg aaaatcgaaa actttggttt gcgaaaatcg
     4201 gaaacaagtt tgtgattaac gaaatcggtc aaaaagctat aaaatcaatg ttttttaccg
     4261 aaaacgaaaa ctcaaatcaa caaccagttg gcgaaaaatc gaaaaccgaa aaccataaaa
     4321 acgccaacca agtttccgaa ttagaactct tttttttcct agaaaaacaa ttaaatcaaa
     4381 aagatataca gctacaagaa aaagacgaac aattaaaaca aatgcaaaaa ttactcgacc
     4441 agcaacaaca actcacctta caagccaatc aacaaatcaa aaaacttcac gaacaactcc
     4501 agctaaccta tgcagaagaa tcttcagaaa aagaacccct ttctgttact gaagaaactc
     4561 aaaaaggaca aaacaaggag aaaaaatggt ggcaattctg gcaataaccc aacaattttt
     4621 attcataaaa attgttgggt tactttcttt ttttgctttt acaagcatag cgacatgatc
     4681 tcacgtcgct attttttaaa gagcataagg aaacgcggta tcactttctt acgaaaggtg
     4741 ctaggtaggc tgtgcctacc acgtttctaa gaaaaaaatt tgctatcact ttttcgccat
     4801 tgtggctagc ttatgtatag tatttcggta aaaaacagct atacaaatac tatatttgta
     4861 tgatatacat aaattataca cacataagaa aggacgtttt ctatggacat tttagttcga
     4921 aatatcaatc caaaaattat tacaagagta gataaacgtt gtcaggaact tacaaccaaa
     4981 acaggagaaa aatggagtcg aaataaatat ttaaaagtgc taatcgaaag tgattttgac
     5041 cgtcaactta tgaattacaa aaaagagcaa ttcgatttat tactcgaaaa atttatcgat
     5101 attcaaacaa aaaatactga aacattgaac gactatgttc atacaaacaa ccaactcatt
     5161 gaaacactta tcggaggaat ataacatgag aaaaacttct gttcaaaaac acgttaccct
     5221 ttatcccagt cacattgacg ttttagattc catcatcgaa aaaaatccag atatcaataa
     5281 ctactcggaa gccattcgct ttttatgtat gagttatgaa cctgatacca atacaacaac
     5341 taccagtaac caagccaaac taaatgcgat gggaaaagaa atttctatca tgaccgaaat
     5401 tatcgcagaa tttgctgatt tgtatttaaa agatacaggc gttcttatcg gtacaaactc
     5461 gaaagtatat caagaagtaa aagaaaaaat tgaaaaacag ctaaatccct cccaacgaaa
     5521 tcaaccaaag aatcgtcaaa aagaaacgag tcaaacattc acttctaaaa atttcgaccc
     5581 aaccaacttt ctaaaaagat aaaaaaataa accaggaaat ttttaatttc ctggtttatt
     5641 ttctagagag cggtcttcca ataagatttg caaatcgatc ccaatttgcg taaaaaaaaa
     5701 gctgctcgta cgagcagcta aacaagaaaa agtcagcttg ttttaagggt aaaataagtg
     5761 taacaaattt tgcgaatgca acgatctttt ttattatacg caaacaatc
//
LOCUS       DARBET010000117         5790 bp    DNA     linear   BCT 07-NOV-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4027
            SAMEA114569841-rid20112183.denovo.121, whole genome shotgun
            sequence.
ACCESSION   DARBET010000117 DARBET010000000
VERSION     DARBET010000117.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA114569841
            Sequence Read Archive: ERR12203666
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 5790)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5790)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001972618.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001972618.1
            Genome Coverage       :: 132x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/07/2023 13:18:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,041
            CDSs (total)                      :: 2,975
            Genes (coding)                    :: 2,908
            CDSs (with protein)               :: 2,908
            Genes (RNA)                       :: 66
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 67
            CDSs (without protein)            :: 67
            Pseudo Genes (ambiguous residues) :: 0 of 67
            Pseudo Genes (frameshifted)       :: 26 of 67
            Pseudo Genes (incomplete)         :: 28 of 67
            Pseudo Genes (internal stop)      :: 20 of 67
            Pseudo Genes (multiple problems)  :: 6 of 67
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5790
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA114569841-rid20112183.denovo.121"
                     /isolate="VRE4027"
                     /db_xref="taxon:1352"
                     /country="Denmark"
     gene            complement(99..1229)
                     /locus_tag="SCJ86_002689"
     CDS             complement(99..1229)
                     /locus_tag="SCJ86_002689"
                     /EC_number="2.1.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008268652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-methyltetrahydropteroyltriglutamate--
                     homocysteine S-methyltransferase"
                     /protein_id="HEG1170176.1"
                     /translation="MSKNRPFTVDHVGSFLRPQSIKEARKKVQEGLLSKEELRAIEDQ
                     EIKKLVEDQKAVGIKGITDGEFRRGFWHLDFLEELNGIEGYVPETGYNQTFHGKAAPA
                     YNIRVVDKLSFNPNHPFLKDFAFLKEVVETDDTFVAKATIPSPTMILRQEILSNDGSS
                     KIKEIYPDLSDFYEDLSSTYKAAIHAFYEKGCRYLQFDDTNWAFLADQTKREALLQKG
                     IDPKEMAHICTEIINNALEDKPEDLTITTHICRGNHASSWLFSGGYEPIAEELFSTNY
                     DGFFLEYDSDRAGDFTPLRHWSNKESKVVLGLVTSKFPDLEDTEKIKERIKEAQQFVP
                     LENLSISPQCGFASTEEGNHLTEEEQWKKIQLLQQVAKEVWL"
     misc_binding    complement(1288..1515)
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00230"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="T-box leader; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="tRNA"
                     /db_xref="RFAM:RF00230"
     gene            complement(1877..3079)
                     /locus_tag="SCJ86_002690"
     CDS             complement(1877..3079)
                     /locus_tag="SCJ86_002690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317927.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HEG1170177.1"
                     /translation="MPKIKNIYQDKKTKKWFFRAYLGIDENGRKVQKTKRGYASQKDA
                     KIAYDKYMETHDFNKTIPNQLCSTNQMTFEEFYKDRFVKWYERQVKSQTYENAQFIFE
                     KRMQFFYHFRVRDITSHDIEDWLFELSQTESRNSRKKVNGETLSKSYINRIRGHLKIV
                     LDRAVKEGVIAKNPVEDVSSLPSENKKVEFWEHSEFQKVMNELKDDKIQTHHRKIVYE
                     MLFYTGIRIGELEALSWSNVDFEKNTITIEKTLIYNTKDDWYFSTPKTKCAYRTIGLG
                     KKLSLKLRQWYDLQKRIGNFEYVAQLDGTFTPPYSFANWLKEAAKKAGVKPIKLHALR
                     HSHVAFLIEQNIQPLSIQERLGHANIQITLGTYGHLYAKSDQQVVSAIDNVQEGAILS
                     KNDNLLPV"
     gene            complement(3139..3342)
                     /locus_tag="SCJ86_002691"
     CDS             complement(3139..3342)
                     /locus_tag="SCJ86_002691"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295027.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3173 family protein"
                     /protein_id="HEG1170178.1"
                     /translation="METKCDFMVNRAILIEMGFKPSQAARMIKESKAYLARIEGIDFY
                     NNRQVGVVPSRVIEHLFHIQVAE"
     gene            complement(3484..4716)
                     /locus_tag="SCJ86_002692"
                     /pseudo
     CDS             complement(3484..4716)
                     /locus_tag="SCJ86_002692"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016173388.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation factor domain-containing
                     protein"
     gene            4879..5691
                     /locus_tag="SCJ86_002693"
     CDS             4879..5691
                     /locus_tag="SCJ86_002693"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287017.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HEG1170179.1"
                     /translation="MEIDKQAVGNRIRQIRQELKLSMEKFGKLIGDLPRSTVNNWERG
                     INLPKTETLHQIAEIGHVTNEYLLYGDQENQYILEMLQKKAGKLDPKIEGLIVDEIKH
                     ADLVSEKEMDRMIEFFITNLIPPTEQDQFTFQCIDEKKQLYLGSTNFGKEAQLYLQHD
                     NQNHILHMMPFTFSNFSVDRILVYLANQKSYSYFGKHLPKELGEKAILLYSINQLTSD
                     VRIAPLVYSEETASYQYTEDNQYLLEQQYLYLPFVMEVEKERLLNAAYPSSK"
BASE COUNT         1717 a         1189 c          921 g         1963 t
ORIGIN      
        1 aaataaataa tataaaaggt aaataaaaaa gagtggattt ctccactcca tgaaaagccg
       61 actttttggg acgctttttt ttgccaagcc tcacattttt atagccaaac ttcttttgcg
      121 acttgctgca ataattggat tttcttccat tgttcttctt ctgttaaatg gtttccttct
      181 tctgttgaag caaacccaca ttgaggactg atacttagat tttccaatgg tacaaattgt
      241 tgtgcttctt ttattcgttc ttttattttc tccgtatctt ccaaatctgg aaacttagaa
      301 gtaactaatc ctaaaacaac tttactttct ttgttactcc aatgtcttaa aggagtaaaa
      361 tctcctgctc ggtcagaatc gtattccaaa aagaaaccat cataatttgt tgaaaacaac
      421 tcttctgcaa tgggttcata tccaccggaa aacagccaag aagaagcgtg attcccgcgg
      481 cagatatgtg ttgtgatcgt caaatcttct ggtttgtctt ccagtgcgtt attgataatc
      541 tctgtacaaa tatgggccat ttctttcgga tcaattcctt tttgaagaag tgcttctctt
      601 ttagtttgat cagctagaaa cgcccaattt gtatcatcga attgcaggta acggcaacct
      661 ttttcataaa aagcatgaat cgctgctttg taagtagagg acaagtcctc ataaaaatca
      721 gatagatcag gataaatctc cttgattttt gaagaaccat cattagatag gatctcttgt
      781 cgtaaaatca tcgtgggact agggatcgtt gcttttgcaa caaaagtatc atctgtttcc
      841 actacttctt tcagaaaagc aaaatctttt aaaaatgggt gatttggatt gaaagataat
      901 ttatctacta cccgaatatt ataagctggt gctgcttttc cgtggaatgt ttgattatat
      961 ccagtttctg gaacatagcc ttcgatacca tttagttctt ctaaaaaatc aagatgccaa
     1021 aatccacgcc gaaattcacc atctgtaatt cctttgatgc cgactgcttt ttggtcttcc
     1081 actaattttt ttatttcctg gtcttcgatt gcacgaagtt cttcttttga aagtaagcct
     1141 tcttgaactt tttttcttgc ttctttgatc gattgcgggc gtaagaaact tccgacatga
     1201 tctactgtaa atggtctatt tttgctcatt tcactcactc ttttctttgt tttttgtaaa
     1261 aaaaatcctt ttactagcaa atactagtaa aaggacgatt atactcgtgg taccaccttt
     1321 tttcagaaaa aacattcttt ctctcagcct gacaataaaa tcagtggtct tggtaacgaa
     1381 agactgttcg tttttgccta cctttcgaca aaaaatcctc taaggccatt ttcgacagtc
     1441 aacttctagc tttttacacc aaccaaagcc tctcttgaac cattttctgt ctactttcct
     1501 tctcatcgga tactattaga ataaaattag aatagcatgt ttgaaagaaa agtcaatagg
     1561 gaataagaac aaacaaaaac taatttgtta ttcttagtaa tttgtttgtg gaatacaata
     1621 aagcttttct aatatgataa ttgttcagtc gtcctttcat cttagatcac ttccttaaaa
     1681 tgatcatacc cgtttcaacc acataaaaaa ggacttaatt ttgaaaaatt tcccatcaaa
     1741 actaagtcct ttttattata gttatgcaaa tttgcttatt ttctcgctat ttccgataca
     1801 tcttgaacag gagcaattcg actgaaaaca ctggtgcaaa agcatttatt tgtcttatgg
     1861 tagaaccgtg gaaaactcag actggtaata aattgtcatt ttttgaaaga atagcgccct
     1921 cttgaacatt atcaatcgca cttaccactt gttgatctga ctttgcatat aagtgaccgt
     1981 aagttcctaa agtgatttga atattcgcat ggcccaatcg ttcttgaata gatagcggtt
     2041 gaatattctg ctcaattaga aatgcaacat gagaatgtcg tagcgcgtga agcttaatcg
     2101 gtttgacacc cgctttttta gcagcttctt ttaaccagtt cgcaaaagaa taaggtggcg
     2161 taaatgttcc gtccaactga gcaacatatt caaaatttcc aatacgtttt tgcaagtcat
     2221 accactgtct gagttttaag gaaagcttct tccccaaccc aatcgttcga taagcacatt
     2281 tagttttggg agtcgaaaag taccaatcat cttttgtatt ataaatcaat gtcttctcaa
     2341 tcgttatcgt gtttttctca aaatctacat tcgaccaaga caaagcttca agttctccaa
     2401 tccgaatgcc agtataaaag agcatttcat acacaatctt ccgatggtgg gtttgaatct
     2461 tatcatcctt caattcattc atcacttttt gaaactcact gtgttcccaa aactcaactt
     2521 tcttattctc agaaggaagc gaagaaacat cttcaacggg atttttagca atcaccccct
     2581 ctttaacagc ccgatccaat acaattttta ggtgccctcg aatccgattg atataagatt
     2641 ttgataacgt ttcgccatta acttttttcc gtgaattacg tgactctgtc tggcttagtt
     2701 caaataacca atcctcaata tcgtgacttg taatatctcg aacacgaaaa tgatagaaaa
     2761 actgcatacg cttttcgaaa atgaattgcg cattctcata tgtttgactt ttgacctgtc
     2821 tctcgtacca ttttacaaac ctatccttgt aaaactcttc gaaagtcatt tgattggtag
     2881 aacacagttg attaggaatt gtcttgttaa aatcatgtgt ttccatgtac ttgtcataag
     2941 caattttggc atctttttgt gaagcataac ctcgtttagt cttctgaacc tttcttccat
     3001 tttcatctat acctagatac gctcggaaga accatttttt tgtcttttta tcttggtaaa
     3061 tatttttaat ttttggcatc gttccattcc tccctgtact tgagaaaagc tactttaata
     3121 gtaccgcgct tgtactgttt attccgcaac ttgaatgtga aaaagatgtt caattactcg
     3181 ggatggcacc acaccaactt ggcgattatt ataaaaatca ataccttcaa tccttgcaag
     3241 atatgccttg ctctctttaa tcattcgtgc agcttgacta ggtttaaagc ccatctctat
     3301 caaaattgca cgatttacca tgaaatcaca ttttgtttcc attctctcac ctcctgattc
     3361 tttactcctt ttaaaataat cctctttata atctattgtt tctcattcga tggttgtgtt
     3421 atactcgtct tgtcgaggcg gagtacgttg cacaacttgt tgtgtaagca gtactttgct
     3481 tttttagtag cttgttttct catttgatat actttgtaat tttcgttcat aatactcaac
     3541 ctcctcctga taatactccg ggtctgcctt catattttgg agaagtttga tctgtcttgg
     3601 attcatttct ccttgttcaa taatcaaagg caacaaggaa gaatgaaacg tttgatcgac
     3661 ctgtgcaata aaaactaatg atggtgaaac accatgaatt aaccaactca tagttttctc
     3721 aaaactttga ggaattgtat ctaaagaaat ggtgattgcg ccataattgc taataaaggc
     3781 tgcccatttg gcatcaactt ttgcacctag atcatttgga tcacggtcat aaaaacaaat
     3841 tgctccatta agtaactcaa agaacagttt ggttaaatca tgggtttttg aaataatctt
     3901 tgccaagctt tgcgctttct cttttcgata ccgtagttca aagcgattct ttacttttgc
     3961 ctcttctaaa ggaattcctc gtcgcttccg ttgttcatgg tccttctgat aaaagcaaaa
     4021 acgacactga ctcttctttg agcctaaata caaggtctca ccatttgatt cattgtttat
     4081 gatatccgta ccgccatgag attcactcac ttgaaaagtg gtccacacat ggcctagtat
     4141 taccttatgc ttaagttctg gaatgctgag actccccaca aaatcatcta aagtaaaatc
     4201 aatccgtgta aagttcccct gataatctaa taccttacga aaaaactgtt gccaatttgt
     4261 ttttcgtgtt tttagccatc ttgctaagtg tttacagcct tgacctgaaa actccatcac
     4321 agttccttta accgcatcgt caatggaata ccgaatcgtg attacttgat tccacacgta
     4381 ttggccaata tagccctttg gcgcataatc cagttcctca aaacgttcaa tgggcatttc
     4441 aataatctct tcaattagct ttaaatgttg attcgagaaa aagtgaatgg ttagataatc
     4501 aatcaaaaca cggttaactg gaacgacctc ttcttgcaaa ctatccaaca caagattcaa
     4561 tttttcttca cttaattgtc tcgttccttt ttctactttc gataataact atctcgaaat
     4621 ccccactttt tctgctaaat atcgttgtgt ccaccccctt tcttctcgag aaactcgaat
     4681 ggcttcaccg agatcatgtt gattaacttg gttcatcgac aaacctcctt ctgctattta
     4741 cttgtcaaag aacctattga acttatttta agtacatttt tattgtacgc ctgtatttat
     4801 tttaagtcaa tatgttttga ctttttttaa gtacacacgt atactaaatt taagaactta
     4861 tcctgaggag gagttttcat ggagatagac aagcaagcag tcggcaatcg aattcggcag
     4921 attcgccaag aattaaagtt gagtatggag aaatttggca aactaattgg tgatttaccg
     4981 cgaagtacag tcaataactg ggaacgtggg atcaaccttc caaaaaccga aacccttcac
     5041 caaatcgcag agataggtca tgtcaccaat gaatatcttt tgtatggtga tcaagagaat
     5101 caatacattt tagaaatgct ccaaaaaaag gcaggtaagc ttgatcctaa gattgaaggt
     5161 ctcatagtag acgaaatcaa acacgctgac cttgttagtg aaaaagaaat ggatcgaatg
     5221 attgaatttt ttattacaaa tctcattcca ccaactgaac aagaccagtt tactttccaa
     5281 tgtatcgatg aaaaaaagca gctttatctt ggttcaacta attttggtaa ggaagcccag
     5341 ctctatttgc aacatgataa tcaaaatcat attctgcaca tgatgccctt taccttctcc
     5401 aactttagtg tggatcgcat actcgtctat ttagcaaatc aaaaatccta ttcctatttt
     5461 gggaaacatt tacccaagga actaggtgag aaagccatcc ttctatactc aattaatcaa
     5521 ttgaccagcg atgtgcgaat tgccccgttg gtctattcag aagaaaccgc ttcttatcag
     5581 tacacagagg acaatcaata tctactagaa cagcaatatc tttatctacc attcgtgatg
     5641 gaagtcgaga aagaacgcct gttaaatgca gcgtatccat catcaaaatg aggtgacaaa
     5701 attgataagc acgtgacaaa gaaaatactg ctaggacact ccccaatggg tagttgtcac
     5761 agtttcctgt tctttgttag ccccctgtta
//
LOCUS       DARBET010000118         5760 bp    DNA     linear   BCT 07-NOV-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4027
            SAMEA114569841-rid20112183.denovo.122, whole genome shotgun
            sequence.
ACCESSION   DARBET010000118 DARBET010000000
VERSION     DARBET010000118.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA114569841
            Sequence Read Archive: ERR12203666
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 5760)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5760)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001972618.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001972618.1
            Genome Coverage       :: 132x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/07/2023 13:18:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,041
            CDSs (total)                      :: 2,975
            Genes (coding)                    :: 2,908
            CDSs (with protein)               :: 2,908
            Genes (RNA)                       :: 66
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 67
            CDSs (without protein)            :: 67
            Pseudo Genes (ambiguous residues) :: 0 of 67
            Pseudo Genes (frameshifted)       :: 26 of 67
            Pseudo Genes (incomplete)         :: 28 of 67
            Pseudo Genes (internal stop)      :: 20 of 67
            Pseudo Genes (multiple problems)  :: 6 of 67
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5760
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA114569841-rid20112183.denovo.122"
                     /isolate="VRE4027"
                     /db_xref="taxon:1352"
                     /country="Denmark"
     gene            502..1059
                     /locus_tag="SCJ86_002694"
                     /pseudo
     CDS             502..1059
                     /locus_tag="SCJ86_002694"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303250.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="LCP family protein"
     gene            1117..3156
                     /locus_tag="SCJ86_002695"
     CDS             1117..3156
                     /locus_tag="SCJ86_002695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313732.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding transpeptidase
                     domain-containing protein"
                     /protein_id="HEG1170180.1"
                     /translation="MKRSDKHGKNRTGAYIAGAVILIAAAGGGYFYYQHYQETQAVEA
                     GEKTVEQFVQALNKGDYNKAAEMTSKKAANKSALSEKEILDKYQNIYGAADVKGLQIS
                     NLKVDKKDDSTYSFSYKAKMNTSLGELKDLSYKGTLDRNDGQTTINWQPNLVFPEMEG
                     NDKVSLTTQEAARGNIIDRNGEPLATTGKLKQLGVVPSKLGDGGEKTANIKAIASSFD
                     LTEDAINQAISQSWVQPDYFVPLKIIDGATPELPAGATIQEVDGRYYPLGEAAAQLIG
                     YVGDITAEDIDKNPELSSNGKIGRSGLEMAFDKDLRGTTGGKLSITDADGVEKKVLIE
                     HEVQNGKDIKLTIDAKAQKIAFDSLGGKAASTVATTPKTGDLLALASSPSYDPNKMTN
                     GISQEDYKAYEENPEHPFISRFATGYAPGSTFKMITAAIGLDNGTIDPNEVLTINGLK
                     WQKDSSWGSFKTTRVSSDVSQVDLKTALIYSDNIYAAQETLKMGEKKFRTGLDKFIFG
                     EDLDLPISMNPAQISNEDSFNSDILLADTGYGQGELLITPIQQAAMYSVFTNNGTLVY
                     PKLIADKETKDKKNVISETAVQTIVPDLREVVQDVNGTAHSLSALGIPLAAKTGTAEI
                     KEKQDVKGQENSFLFAFNLDNQGYMMVSMLENKEDDDSATKRASELLQYLNQNYQ"
     gene            complement(3265..4416)
                     /locus_tag="SCJ86_002696"
     CDS             complement(3265..4416)
                     /locus_tag="SCJ86_002696"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344252.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation:proton antiporter"
                     /protein_id="HEG1170181.1"
                     /translation="MEFIGILCLILISTTIGSHISRRLGIPAVIGQLLVGVLLGQAGL
                     NWVKPDILVHDFSEIGVILLMFLAGLESDLSLLKKYFRPGMFVALLGIIFPVVFGWLT
                     GKAFQVSGNEAIFFGIILAATSVSISVEVLKELNVVNTKEGSTILGASVVDDILVVLV
                     LSFSLSFLTGEASSNASLPLVLIEQLIYFVFIFLLVKWIAPFLLSFAEKVYANSSIII
                     VSLVICLGMSYVADLVGLSSVIGAFFAGIAVSQTKVKEEVYHNVEALGYAVFIPVFFV
                     SVGLEVDFSDFSEQLLFISILTLVAILTKLLGGYFGAQIAGFSQHSSLMVGAGMISRG
                     EMALIILQIGQQSQLIEPQYYSPLVIVILLSTLISPLVLKYFTKKVYEV"
     gene            complement(4570..5100)
                     /locus_tag="SCJ86_002697"
     CDS             complement(4570..5100)
                     /locus_tag="SCJ86_002697"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF308 domain-containing protein"
                     /protein_id="HEG1170182.1"
                     /translation="MFETIKQNIQKYALLRAAVYIISGIAIVLNPSAVFHFIGYLITA
                     YLVLLGLLNLFEAYRFKRKTGSWSIGLFSGLFFLILALIVYAFAPAIVSILPILLGLA
                     VIINSLFQLFFSMNTKAKSWSVYSSILLLIGGFVLLFNPFKSLMLLFQIFGFILIFMG
                     IQEIINFIRIRKMTIR"
     gene            complement(5199..5549)
                     /locus_tag="SCJ86_002698"
     CDS             complement(5199..5549)
                     /locus_tag="SCJ86_002698"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339248.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2200 domain-containing protein"
                     /protein_id="HEG1170183.1"
                     /translation="MAQNRIFTTSFASVYPMYVAKAERKDRIKEEVDEIICWLTGYSQ
                     TELEYQLQKQVTFEQFFAEAPVPNPSRSLITGVVCGVRVEDIEDNLMKEIRYLDKMID
                     ELAKGKKMEKILRS"
BASE COUNT         2082 a         1055 c         1070 g         1553 t
ORIGIN      
        1 cacataaaac taaaaaatta tcaaagaatc ctggtgcaac tgaaagaatt tgcaacggga
       61 ttctttcaac ttatttgtta tttttttcta aaaaatatta aagattgtta aatttcacta
      121 tttcatttca aattccattg cctaatcatt aggaaaagtc tatcatgaac tcgtaaatag
      181 aatatagtaa taaccttgtt aggtgaggct cctatacaaa cataggctgt tgcccagaaa
      241 cgtcgaaagt cgccaatggg taaaacagaa actgttggat taaggctttt tccaatagct
      301 aagaatgttt tctttacgtt gtatagtgct aaaactcgga cgatgagatg atgctaggtc
      361 agaattcttg ttcgaaaagg tttctgacct tttctttata tttttgtgac tgacttattt
      421 gatgagacaa actcgctcct cacctgacaa gtggattgct gtgtgaggag gagatgcggt
      481 gaaaaaagat cgattggtgg aggtcgctcg gattcacttc tggttgcaca atacaaccag
      541 aaaacacaac aagcaaaact gatctctatc atgagagatt catatgtcga tattccaggt
      601 tacggaatga ataagataaa tgcagcttac tcttatgggg gagttgattt attaaatcaa
      661 acattaaagg aaaatttcaa atttgaagct ccctattatg caagtatcac atttcaagat
      721 tttatcgatt gcgttaatga actgtttcct ggtggagtca agatcgatgc agaaaaatct
      781 ctagatttag atggcgtata tataaacaaa ggagagcaag tgatggatgg taatacttta
      841 ctccagtatg ctcgatttcg ggaagacaaa gaaggagact tcgggaggat cagaaggcag
      901 cagcaagtga tcaaagctat ctctcagcag ctgaaagacg taacttcaat attcaagcta
      961 cccaaagcgg ttgggaaatt acttggtagt atccaaacga accttccaga aagtgtgctt
     1021 ctggattgtg gtatggattt cctcaaagac atatgttaga ataaacaggt ataaatagtg
     1081 aaataaagga atgacaagca agagaaggag gaaaaaatga aaagaagtga caagcacggc
     1141 aaaaatcgaa caggcgctta tattgccggc gcagtgatct taatagcagc tgcgggtggc
     1201 ggttattttt actaccagca ctatcaagaa acccaagcag tagaagctgg agaaaagaca
     1261 gttgagcaat ttgtccaagc tttgaacaag ggagattata acaaagctgc agaaatgaca
     1321 tcaaaaaagg cggcaaataa aagtgcatta tctgaaaaag agatcttaga taaataccag
     1381 aatatatacg gtgctgctga tgtcaaagga cttcagatat cgaatctaaa agtagataaa
     1441 aaagacgatt ctacttacag tttttcatat aaagcaaaga tgaatacctc attaggtgaa
     1501 ttgaaagatc tttcttataa aggaacatta gacagaaatg atggtcaaac cacgatcaac
     1561 tggcaaccca acttggtttt tccagaaatg gaaggaaatg acaaagtaag cctgaccaca
     1621 caagaagcag caagagggaa tattatagat cgaaatggtg aaccattagc aacaacaggc
     1681 aaactaaaac aattaggagt cgttccaagt aaacttggag atggaggcga aaaaacagcc
     1741 aatatcaaag cgattgcttc ctccttcgac ttaacagaag atgctatcaa tcaggcgatt
     1801 tcacaaagct gggtacaacc cgattacttt gtcccattga aaatcattga tggagcaacg
     1861 ccagaacttc cagctggagc taccattcaa gaagtagacg gcagatatta tcctttgggt
     1921 gaagcagctg ctcagctgat tggttacgtg ggagatatca cagcagaaga tattgataag
     1981 aatccagaat taagcagtaa tggaaaaatc ggtcgctcag gtttggagat ggcttttgat
     2041 aaggatcttc gtgggacaac aggtgggaaa ttaagcatta cagatgcaga cggtgtagag
     2101 aaaaaagttc tgatcgaaca tgaagttcaa aatggcaaag atatcaaatt gacaatcgat
     2161 gcgaaggcac aaaaaatagc ttttgacagt ctaggaggaa aagctgcatc aactgttgcg
     2221 acaacgccaa aaaccggtga tcttcttgcg cttgctagct ctccaagcta tgatccaaac
     2281 aaaatgacaa acgggatctc acaagaagat tacaaagctt atgaagaaaa tcctgaacat
     2341 ccattcatca gccgatttgc gacaggttat gctcctggat ctacgtttaa aatgattaca
     2401 gcagcaatcg gtctcgacaa cggcactatc gatccaaatg aagtgttgac gatcaacggg
     2461 cttaaatggc aaaaagacag ttcttgggga tcttttaaaa caacgcgtgt cagtagtgat
     2521 gtatcacaag tagacttaaa aactgctttg atctattccg ataatatata tgcggcacaa
     2581 gaaacgttga aaatgggtga gaaaaaattt cgtacaggct tagataaatt catttttggt
     2641 gaagaccttg atttgccaat tagtatgaat ccagcacaaa tttctaatga agatagcttt
     2701 aactcagata tcttgctagc tgatactgga tatggacagg gcgaacttct aattactcct
     2761 atccagcaag cagcaatgta ttctgttttt accaacaatg gcacacttgt ctatcctaaa
     2821 ttgattgcag ataaagagac aaaagataag aagaatgtca tcagcgaaac agcagtacaa
     2881 acgatcgtgc cagatctgag agaagttgtg caagatgtaa atggtacagc acattctctt
     2941 tctgctttag ggattccgtt ggcagcgaaa actggtacag cggaaatcaa agaaaaacag
     3001 gatgtaaagg ggcaagagaa cagtttcttg tttgctttca atcttgataa ccaaggttat
     3061 atgatggtca gcatgttgga aaataaagaa gatgatgatt cagcaaccaa acgagcatcg
     3121 gaactattgc aatacctcaa ccaaaattat caataaaaaa ttgcttttta agtacagtaa
     3181 aaggactgtg gcgttatcgt cacagtcctt ttttcaaata aacggttttc acgatgtctc
     3241 tttcacaact tctttttgat ttactcacac ttcataaact ttttttgtaa aatatttgag
     3301 aacaagtgga gaaataagtg tacttaataa aatcacgata actaaaggtg aatagtactg
     3361 tggttcgatc aattgacttt gctgtccaat ttgtaaaatg atcaaagcca tttctccacg
     3421 cgaaatcata ccagcaccga ccatcaatga actatgttga gaaaaaccgg caatctgagc
     3481 accaaaataa cctccaagca atttggtcaa aatcgctaca agcgtcaata tagaaataaa
     3541 gagtagttgt tcactaaagt cagaaaaatc aacttctaat cctacactga caaaaaatac
     3601 tggtataaat actgcatatc ctaaagcctc tacattgtgg tatacttctt ctttaacctt
     3661 tgtctgactg acagctatcc cagcaaaaaa agcaccaatg acagagctaa gcccaacaag
     3721 atccgctacg taagacattc ctaaacaaat aactagcgat acgatgataa tagaagaatt
     3781 tgcgtacact ttttctgcaa atgaaagtag gaaaggcgca atccatttga ctaataagaa
     3841 aataaaaaca aaataaatta gctgttcaat caaaacaagc ggtaaagaag cattgctaga
     3901 agcttccccc gttagaaaag ataggctgaa acttagaact aaaacgacta aaatgtcatc
     3961 taccacagaa gcacccaaaa ttgttgatcc ttcttttgta ttcactacgt ttagttcttt
     4021 taatacttct actgaaatac tgacagaagt tgccgcaaga ataatcccaa agaaaatcgc
     4081 ttcatttccc gaaacttgga atgctttgcc tgtcaaccag ccaaaaacaa ccgggaagat
     4141 gatcccaagc aaagcaacga acataccagg tcggaaatat tttttcaaaa gagataaatc
     4201 actttccaga ccagctaaaa acattaataa gatcacacca atctccgaaa agtcatggac
     4261 tagaatatca ggtttgaccc aattcaatcc agcttggcct agcaaaacac caacaagtaa
     4321 ttgtccaatc actgctggga tacctaatct ccgcgagata tgtgagccaa tcgttgttga
     4381 aatcaatatc aggcataaaa taccaataaa ttccattctc atcattcctt tgtataaaat
     4441 atagcccgaa cttagcataa catacattat tgaaaaaata ggaaaatata ctcacaaaaa
     4501 aaccaacaat ccaaaagatt tttttggatt gttggttttt atacggtcta ttttaataac
     4561 taaaaatgat taacgtatag tcatttttct tattctgatg aaattgataa tctcctggat
     4621 gcccatgaaa atcaaaataa agccgaatat ttggaataga agcatcaaac ttttgaatgg
     4681 attgaacaat aagacgaatc cgccaatcaa caacagaata ctactataaa cactccaact
     4741 tttagctttt gtattcatgc tgaagaacaa ctggaacaag ctgttgatga taacagctaa
     4801 tccaagaaga atcggcagga tagaaacgat tgcaggtgca aaagcataga cgatcagagc
     4861 taagatcagg aaaaaaagac cactgaacag accgatactc caagaacctg ttttcctttt
     4921 aaaacggtaa gcctcaaaaa gattcaacaa cccaagcaaa acaagatagg cggtgatcag
     4981 ataaccgata aaatgaaaaa cagcagaagg gtttagcaca atcgcaatcc cagaaataat
     5041 ataaacagca gctcttaata aggcgtattt ttgaatattt tgtttaatcg tctcaaacat
     5101 ataagaaaac ctcctttgac ttattttaac gtgaactagt cattttacca aattaaatgt
     5161 tacaaaatga gcgaaggaga agaatatctc tggaaaaatc aacttctgag gatcttttcc
     5221 atttttttgc cttttgctag ctcatcgatc attttatcta aataacgaat ttctttcatc
     5281 aagttatcct cgatgtcttc tactcgtaca ccacaaacaa cacctgtaat caaggaacgt
     5341 gaaggattgg ggacaggggc ttccgcaaaa aattgttcga aagtcacttg tttttgtagt
     5401 tgatattcta gttctgtttg ggaataaccc gtcagccaac aaatgatttc atctacttcc
     5461 tcttttatac gatcttttct ctccgctttt gctacataca taggataaac gcttgcaaaa
     5521 cttgtcgtaa aaatacgatt ttgcgccata atcagtcacc tttctttttt tcaaattatt
     5581 tactacttat agtatataag aaaaagagaa gaaaaagcga ttattcaaaa aaacaaaggt
     5641 tctataatat tttgtgttgg tggaaaatcg aaggcgattt tctactaaca ctttttcttc
     5701 gtttcaactc aaaaagagac tcacttttgg taaaatttag tcgaccaaaa cgtcatccta
//
LOCUS       DARBET010000119         5615 bp    DNA     linear   BCT 07-NOV-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4027
            SAMEA114569841-rid20112183.denovo.123, whole genome shotgun
            sequence.
ACCESSION   DARBET010000119 DARBET010000000
VERSION     DARBET010000119.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA114569841
            Sequence Read Archive: ERR12203666
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 5615)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5615)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001972618.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001972618.1
            Genome Coverage       :: 132x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/07/2023 13:18:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,041
            CDSs (total)                      :: 2,975
            Genes (coding)                    :: 2,908
            CDSs (with protein)               :: 2,908
            Genes (RNA)                       :: 66
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 67
            CDSs (without protein)            :: 67
            Pseudo Genes (ambiguous residues) :: 0 of 67
            Pseudo Genes (frameshifted)       :: 26 of 67
            Pseudo Genes (incomplete)         :: 28 of 67
            Pseudo Genes (internal stop)      :: 20 of 67
            Pseudo Genes (multiple problems)  :: 6 of 67
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5615
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA114569841-rid20112183.denovo.123"
                     /isolate="VRE4027"
                     /db_xref="taxon:1352"
                     /country="Denmark"
     gene            complement(60..2009)
                     /locus_tag="SCJ86_002699"
     CDS             complement(60..2009)
                     /locus_tag="SCJ86_002699"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347433.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp protease ATP-binding subunit"
                     /protein_id="HEG1170184.1"
                     /translation="MENSTEFFKNIQTEFNHALTPMKPPKHKLVGRDDELHELSLILN
                     RPETPQAILLGEAGTGKTALASEWAERQRQDGKKVQVYKLSIGQLRVGGDDVLQTRLE
                     TLIPRLKEYERILKAEDKDNEFVLFIDEFHKVVSSFGSGNKIGGDSLKEPLANESLQM
                     ITATTRKEYENYIKRDDALGRRLQPLELNELKSDVVVDILRQWLNTSSVKDDPQASFR
                     LSQRIPDELLQEIVQANKLYREDLSEPAKSIAVMEDLIAKDRVDGNAYNRESLLQIFG
                     KYNIQLDSDFDMEKLQEELKSIKGQPLAVYTITMLFKRMKLGFDRFKGRKQPKATALF
                     TGSTGVGKTAMAKAVARGLYGDENKVLLVNMPDYKTKDDAMRFRRLIGTHVRHDPSAI
                     IVCDEYEKAGVETLDAFLSILDEGRISFTERGWDDREVHDTVSLSNTILFATTNAGAE
                     MFDSLNRYSSLSRVTNKENSNEYSTELKREYRKVASGIKQALIGEGLKPEVINRFGRI
                     VPFWALSEQTLLEIAYNEMLKSAELVRKHLNIDLRLPPRKERVYGQNFYADDLSMHLV
                     FDMASQEDSKSGGARNILHLVESEFEEAITEAIITYPNAKIFDVKLSEAGAFEGKGDS
                     NAEIIAKPSVAESKENKIVFSIGGA"
     gene            complement(2051..3109)
                     /locus_tag="SCJ86_002700"
     CDS             complement(2051..3109)
                     /locus_tag="SCJ86_002700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010782611.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar biosynthesis protein FliS"
                     /protein_id="HEG1170185.1"
                     /translation="MEKNVNSTSTKDQYSIIGGLLGDKDKRTRHGLRIMLATFLGVLF
                     LLTGLGSYIYQLGNYNYYLVNNTTPIGSSLEFARSGAEITISDVWTDQNRDVTVVKLG
                     YSQNARKLLSTQGKNYNLYMATKEKDRPKKLEMSYGMLSTDGDAFLFLKGKLEERAYQ
                     VFIANQVSLISGDDTADDDTKVNVNEADSVTKALSQYSMNDVNNEGVISFGKKAKPVN
                     TPDNINFRINPYSDSTNVYQGSFLTKDGDIDYSKVVAVTSKNNVVNKLEDQLNASNTN
                     IKKIEATKAEYLERLQQNPDDKDSQESIRQLDSRMETEIKAINDTQKLIDQYKNADFN
                     KDSFGDMKTTFKYLTLTD"
     gene            complement(3228..3683)
                     /locus_tag="SCJ86_002701"
     CDS             complement(3228..3683)
                     /locus_tag="SCJ86_002701"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320776.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEG1170186.1"
                     /translation="MNAINWHEFVNYMESLLDDLKSFSIADAVKHGLPVSTTMKLRGL
                     KIPDEIIKLRTKTIIETAKVVDNMNHELKGKKTTYEKDFERFLKSVIGKYLGKKSFSE
                     LRDEEIVPTPMVSKIRKNKAEGNYYLGTIAKIIDYLENKEQKKGTKKGR"
     gene            complement(4267..5070)
                     /locus_tag="SCJ86_002702"
     CDS             complement(4267..5070)
                     /locus_tag="SCJ86_002702"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320777.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation protein"
                     /protein_id="HEG1170187.1"
                     /translation="MSQEVTEKIIDSDVDLVSEQTNAIVRYNNGLNTTALRGFKPVEM
                     DVFWAILSKMKRQGTAEVTFDFNLFRYLVKYDRREKDSFYSVLKALSDKLGTLTYRFE
                     DSDVYEQLWLFQKFRIDKQKETVTIKANEEFEFILNSIGKNFTRFELDNFTKLRSSYT
                     KELYRHLMQYRNKEWRNGYWVVKVEDFRRALSIPSSYRMTNIDKRILEQAKEEFLAPD
                     ENGIQVFETFDYEKIYAKKGRRVDRLRFTFSEPESNLPTISMHNWLEED"
BASE COUNT         1653 a         1108 c          777 g         2077 t
ORIGIN      
        1 tcttacgacc catcgtttta ttctaaggtt ttgtagtaaa tatttcttga atttcatagc
       61 tacgcccctc caatcgaaaa tacaatcttg ttttctttac tttcagcaac ggaaggcttt
      121 gcaataattt cagcattaga atcacctttt ccttcaaacg ctccggcttc actcaactta
      181 acatcgaata tttttgcgtt tggataagta attattgctt cagtaatcgc ttcttcaaac
      241 tcagattcaa ctaaatgcaa gatattcctt gcaccccctg atttagaatc ctcttgtgaa
      301 gccatgtcaa aaactaagtg catggagaga tcgtctgcat aaaaattttg accatatact
      361 ctttctttcc taggtggaag acgtaaatct atatttaaat gttttcgtac taattcagca
      421 ctcttcaaca tttcattata agcaatttcc agaagagttt gttcggataa agcccaaaat
      481 ggaactattc gtccaaatcg gttaataact tcaggcttta gaccttcacc aatcaaggct
      541 tgtttgatcc ctgatgcgac ttttcggtat tctcttttta gttcagtgct gtattcatta
      601 gaattttcct tgtttgtaac acgagatagt gagctatatc ggtttaacga atcgaacatc
      661 tccgcacctg catttgttgt cgcaaataaa attgtatttg acaatgaaac ggtgtcgtga
      721 acttctcgat catcccatcc tctttcagta aaacttatcc taccttcatc taaaattgat
      781 aaaaaggcat cgagtgtttc tacaccagct ttctcatatt catcacacac aataatcgca
      841 gaaggatcat gccttacatg agtacctata agtcgtctaa aacgcattgc atcatctttg
      901 gttttgtagt ccggcatatt aactaagaga actttatttt catcgccata aagtcctctt
      961 gccacggctt tagccattgc agttttaccg acccctgttg atcctgtaaa cagagcagtt
     1021 gcttttggtt gctttcgacc tttaaaacga tcaaatccaa gcttcatacg tttaaacaac
     1081 attgttatag tataaactgc taacggttgt ccttttatgg actttaattc ctcttgaagt
     1141 ttttccatat caaaatcaga atctagctga atattatact taccaaatat ttgcaataga
     1201 ctttctcgat tataagcatt tccgtctact cgatcttttg caatcaaatc ctccattaca
     1261 gcaattgatt tagccggttc ggacaaatcc tcacgataaa gtttgtttgc ttgtacgatt
     1321 tcttgaagta attcatcagg aatcctttgc gataatctaa aagaagcctg aggatcatct
     1381 ttaacagagg atgtatttag ccattgtctc aaaatatcga cgacaacatc gctttttagt
     1441 tcattcaatt ctaacggttg tagccgtctc cctaaggcat catctctttt gatatagttt
     1501 tcatattctt ttcttgtagt agcggtaatc atttgaagtg attcatttgc taacggttct
     1561 tttagcgaat cacccccaat tttattgcca ctcccaaaag agctaacaac tttatgaaac
     1621 tcgtcaatga agaggacaaa ttcattatct ttgtcctcag cttttaaaat tcgctcatat
     1681 tctttcaatc ttggaattaa tgtttctaaa cgtgtttgga ggacatcatc tcccccaaca
     1741 cgcaattgtc cgattgataa tttatatact tgtactttct ttccatcttg tcgctgtctt
     1801 tcagcccatt cacttgcaag tgctgtttta cctgttccgg cttcacctaa caagatagct
     1861 tgcggtgttt ccggtctatt caagatcagt gacaactcat ggagttcgtc atcacgtccc
     1921 actaacttat gttttggtgg tttcattggt gtgagagcat gattaaattc ggtttgaata
     1981 ttcttaaaaa actcggtact attttccatc tgtttgtgtt cacttcctct aaaataagaa
     2041 taatttactt ctagtcagtc aaagttaggt acttaaatgt cgtcttcatg tctccaaaag
     2101 aatctttgtt aaagtcagca tttttgtatt gatcaatcaa tttttgtgta tcattgattg
     2161 ctttaatttc ggtctccatt cgactgtcta gttgacgaat actttcttgc gaatctttat
     2221 cgtcaggatt ttgttgtaaa cgttccagat actcagcttt tgtggcttca attttcttga
     2281 tgttagtatt tgaagcattt aactgatctt ctagcttatt tacaacatta ttcttacttg
     2341 taactgctac aacttttgaa taatcaatat cgccatcttt ggtaagaaag cttccttgat
     2401 agacattagt tgaatctgaa tatggattta ttctgaaatt gatattatca ggagtattaa
     2461 caggttttgc ttttttccca aaagaaatta ctccttcatt attaacatca ttcatagaat
     2521 attgagaaag tgcttttgta actgaatctg cttcattcac attgactttg gtatcatcgt
     2581 cagctgtatc atcaccagaa attagtgata cttggtttgc aataaaaact tgataagctc
     2641 tttcctctaa tttacctttt aggaataaga aagcatcccc atcagtactt aacatcccat
     2701 agctcatttc aagcttctta ggacgatctt tttcttttgt tgccatatat aagttatagt
     2761 tttttccttg cgtagataac aactttctag cgttttggga gtaaccaagt tttactactg
     2821 ttacatctct attttgatct gtccacacat ctgaaatagt gatttctgct cctgatcgag
     2881 caaactccaa tgaagaccct ataggtgttg tattgttcac gagataataa ttatagtttc
     2941 ctaattgata aatgtagcta cccaagcctg ttaataaaaa aagcactcct agaaaagttg
     3001 caagcataat gcgtaatccg tgacgtgttc tcttatcctt atcaccaagc aaccctccaa
     3061 taatactgta ttgatctttg gtagatgttg aattgacgtt tttttccatc agtttgtctg
     3121 ccccctttca taaaagagca aacaatcaga cgttttttcg gtaattctag gttcgggatt
     3181 ttgaatcatt tcggtatatt cttccccatt aatatttaac acaaaaatta tcttcctttc
     3241 ttcgttcctt tcttttgctc tttattttcc aaataatcaa tgatttttgc tattgtacca
     3301 agatagtaat tcccctcagc tttgtttttt cggattttgc taaccattgg agtaggtaca
     3361 atctcttcat ctctcaattc actgaaagat tttttaccta gatattttcc aataacagat
     3421 tttaaaaaac gttcaaaatc tttctcgtaa gttgttttct tgccttttag ttcatggttc
     3481 atattgtcaa cgacttttgc ggtttcaata attgttttag ttctcagctt aataatttca
     3541 tctggaattt tcaatcctcg taatttcatc gttgtactaa ctggtaaccc atgctttact
     3601 gcatcagcaa tactaaaact ttttagatca tctagtaagc tctccatata gttaacaaat
     3661 tcatgccaat taatagcatt cattgtctca ccccttcatt tttttaaaat gaggttcggg
     3721 cgtaacacat ttatgttatt ataatatcat aataacataa ataaatcaat cagcaaattt
     3781 agatatccct taaagtatca gcaattattc aacaagatat ttattttagc tatttcagtc
     3841 atcataaaag actaattagc aataaaataa ctaatattat ccatttatca gacgaaaaga
     3901 aaaaacttat ctaattactc tatattaact tttaacaaat agttgacttg tccaaaaatg
     3961 ttaccttcaa tctcacaaag atattaagat gtttcctacc aaaactgata tcaccggact
     4021 ttttattctg tgttgtccaa aagtgttacc tttagtcgat caacaaatta aaaaaaattg
     4081 tttttttctt gacttgtcca aaagtgttac ccatagttgt taaaatttgt atagattagc
     4141 cattctttcg aatattattt agactgaaac ttgcttacaa aaaaacttct tgcatttatt
     4201 gtccaaaagt gttacccata cttttttaaa gaagcaggtt gtattaaaat tacaacctgc
     4261 ttcttctcaa tcttcttcta accaattatg cattgaaatg gttggcaagt ttgattctgg
     4321 ctcactaaaa gtgaatctga gccgatcaac tcttcttcct ttctttgcat agattttttc
     4381 ataatcaaaa gtttcaaata cctgtattcc gttttcatca ggagctaaaa attcttcttt
     4441 cgcctgctct agtattcttt tgtcaatatt tgtcattcta taacttgaag gtatacttaa
     4501 tgctcttcta aaatcttcaa ctttgactac ccaataacca tttcgccact ctttatttct
     4561 gtattgcatg agatgacgat acaattcttt tgtgtagcta cttcttagtt ttgtaaaatt
     4621 atcaagttca aaacgagtaa agtttttgcc aattgagttt aagataaatt caaattcctc
     4681 atttgcctta attgtaactg tttccttctg tttatctatc cggaattttt gaaaaagcca
     4741 cagctgttca tagacgtctg aatcttcaaa acggtaggtt aaagtaccta gtttgtcact
     4801 caaagctttt aacacagaat aaaaactgtc cttttctctt ctgtcgtatt tgaccaaata
     4861 acgaaagagg ttgaaatcaa aagttacttc tgctgttcct tgacgtttca tttttgataa
     4921 aatagcccaa aacacatcca tttcaacagg cttaaatcct cgcaaagctg ttgtattcag
     4981 accattgtta taacgcacaa ttgcatttgt ttgttctgat actaaatcta catcactatc
     5041 aataatcttt tcggtaactt cttgagacat tgattactct ccaatcttct ttttgcgctc
     5101 ctttaatcat gttaatctgg tactaattaa aggaaacaat tttttctgct tgttacctaa
     5161 attaaatcac aactaaaggt aacagtcaac aaagatttgt tactaatcta attttctatt
     5221 ttttcgcctt ccaaatcaag aaaaccgaag gtaacgtctt atcctagtgt gatacctcaa
     5281 atgtccactt ttgttacctt tgctgtccaa aagtgttacc cttactgtcc acttttgtta
     5341 cctttactgt ccaaaagtgt tacctttata cccctttaat acttactctc ccaagagatt
     5401 aagcctcccc taacagatat attaacagat atttaacaga tatattaaca gatatttaat
     5461 aaaggaggat tactcctctc ttatttcaaa atcttaaaaa taaaaaatct taaaaaaaac
     5521 taacactatc taggagttta ttttgcttgt gtaactttga ataattgaaa aaaaggaaaa
     5581 ggaggaagct tcagcttccc cttttattta tattt
//
LOCUS       DARBET010000120         5531 bp    DNA     linear   BCT 07-NOV-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4027
            SAMEA114569841-rid20112183.denovo.126, whole genome shotgun
            sequence.
ACCESSION   DARBET010000120 DARBET010000000
VERSION     DARBET010000120.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA114569841
            Sequence Read Archive: ERR12203666
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 5531)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5531)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001972618.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001972618.1
            Genome Coverage       :: 132x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/07/2023 13:18:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,041
            CDSs (total)                      :: 2,975
            Genes (coding)                    :: 2,908
            CDSs (with protein)               :: 2,908
            Genes (RNA)                       :: 66
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 67
            CDSs (without protein)            :: 67
            Pseudo Genes (ambiguous residues) :: 0 of 67
            Pseudo Genes (frameshifted)       :: 26 of 67
            Pseudo Genes (incomplete)         :: 28 of 67
            Pseudo Genes (internal stop)      :: 20 of 67
            Pseudo Genes (multiple problems)  :: 6 of 67
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5531
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA114569841-rid20112183.denovo.126"
                     /isolate="VRE4027"
                     /db_xref="taxon:1352"
                     /country="Denmark"
     gene            complement(<1..985)
                     /locus_tag="SCJ86_002703"
     CDS             complement(<1..985)
                     /locus_tag="SCJ86_002703"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_032495458.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS256-like element IS1542 family transposase"
                     /protein_id="HEG1170188.1"
                     /translation="MTQVHFTLNNEEVQSIIEHSVKDDVSKNILTTVFNQLMENQRTE
                     YIQADDYERSESRQSQRNGYYERDFTTRVGTLELKVPRTRDGEFSPTVFERYQRNEKA
                     LLASMLEMYVSGVSTRKVSKIVEELCGKSVSKSFVSSLTEQLDPMVNEWQNRSLSGTN
                     YPYLMTDVLYIKVREDHRVLSKSCHIAIGITEGGDREIIGFMIQNEESDDTWSIFFEY
                     LKERGLKGTELIISDAHKGLVSAIRKSFTNASWQRCQVHFLRNIFSSIPKKNSKPFRE
                     AVKAIFKFTDIELARTAKNALVGEYIDQPKYTKACEILDNGFEDAFQYTVIG"
     gene            1137..1832
                     /gene="vanR"
                     /locus_tag="SCJ86_002704"
     CDS             1137..1832
                     /gene="vanR"
                     /locus_tag="SCJ86_002704"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001280781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VanA-type vancomycin resistance DNA-binding
                     response regulator VanR"
                     /protein_id="HEG1170189.1"
                     /translation="MSDKILIVDDEHEIADLVELYLKNENYTVFKYYTAKEALECIDK
                     SEIDLAILDIMLPGTSGLTICQKIRDKHTYPIIMLTGKDTEVDKITGLTIGADDYITK
                     PFRPLELIARVKAQLRRYKKFSGVKEQNENVIVHSGLVINVNTHECYLNEKQLSLTPT
                     EFSILRILCENKGNVVSSELLFHEIWGDEYFSKSNNTITVHIRHLREKMNDTIDNPKY
                     IKTVWGVGYKIEK"
     gene            1810..2964
                     /locus_tag="SCJ86_002705"
     CDS             1810..2964
                     /locus_tag="SCJ86_002705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305818.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VanA-type vancomycin resistance histidine kinase
                     VanS"
                     /protein_id="HEG1170190.1"
                     /translation="MVIKLKNKKNDYSKLERKLYMYIVAIVVVAIVFVLYIRSMIRGK
                     LGDWILSILENKYDLNHLDAMKLYQYSIRNNIDIFIYVAIVISILILCRVMLSKFAKY
                     FDEINTGIDVLIQNEDKQIELSAEMDVMEQKLNTLKRTLEKREQDAKLAEQRKNDVVM
                     YLAHDIKTPLTSIIGYLSLLDEAPDMPVDQKAKYVHITLDKAYRLEQLIDEFFEITRY
                     NLQTITLTKTHIDLYYMLVQMTDEFYPQLSAHGKQAVIHAPEDLTVSGDPDKLARVFN
                     NILKNAAAYSEDNSIIDITAGLSGDVVSIEFKNTGSIPKDKLAAIFEKFYRLDNARSS
                     DTGGAGLGLAIAKEIIVQHGGQIYAESNDNYTTFRVELPAMPDLVDKRRS"
     gene            3179..4147
                     /locus_tag="SCJ86_002706"
     CDS             3179..4147
                     /locus_tag="SCJ86_002706"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001059542.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-lactate dehydrogenase VanH-A"
                     /protein_id="HEG1170191.1"
                     /translation="MNNIGITVYGCEQDEADAFHALSPRFGVMATIINANVSESNAKS
                     APFNQCISVGHKSEISASILLALKRAGVKYISTRSIGCNHIDTTAAKRMGITVDNVAY
                     SPDSVADYTMMLILMAVRNVKSIVRSVEKHDFRLDSDRGKVLSDMTVGVVGTGQIGKA
                     VIERLRGFGCKVLAYSRSRSIEVNYVPFDELLQNSDIVTLHVPLNTDTHYIISHEQIQ
                     RMKQGAFLINTGRGPLVDTYELVKALENGKLGGAALDVLEGEEEFFYSDCTQKPIDNQ
                     FLLKLQRMPNVIITPHTAYYTEQALRDTVEKTIKNCLDFERRQEHE"
     gene            4140..5171
                     /gene="vanA"
                     /locus_tag="SCJ86_002707"
     CDS             4140..5171
                     /gene="vanA"
                     /locus_tag="SCJ86_002707"
                     /EC_number="6.1.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001079845.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanine--(R)-lactate ligase VanA"
                     /protein_id="HEG1170192.1"
                     /translation="MNRIKVAILFGGCSEEHDVSVKSAIEIAANINKEKYEPLYIGIT
                     KSGVWKMCEKPCAEWENDNCYSAVLSPDKKMHGLLVKKNHEYEINHVDVAFSALHGKS
                     GEDGSIQGLFELSGIPFVGCDIQSSAICMDKSLTYIVAKNAGIATPAFWVINKDDRPV
                     AATFTYPVFVKPARSGSSFGVKKVNSADELDYAIESARQYDSKILIEQAVSGCEVGCA
                     VLGNSAALVVGEVDQIRLQYGIFRIHQEVEPEKGSENAVITVPADLSAEERGRIQETA
                     KKIYKALGCRGLARVDMFLQDNGRIVLNEVNTLPGFTSYSRYPRMMAAAGIALPELID
                     RLIVLALKG"
     gene            5177..>5531
                     /locus_tag="SCJ86_002708"
     CDS             5177..>5531
                     /locus_tag="SCJ86_002708"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000402347.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-Ala-D-Ala dipeptidase VanX"
                     /protein_id="HEG1170193.1"
                     /translation="MEIGFTFLDEIVHGVRWDAKYATWDNFTGKPVDGYEVNRIVGTY
                     ELAESLLKAKELAATQGYGLLLWDGYRPKRAVNCFMQWAAQPENNLTKESYYPNIDRT
                     EMISKGYVASKSSHSR"
BASE COUNT         1669 a         1078 c         1251 g         1533 t
ORIGIN      
        1 ttccgataac cgtgtattga aaggcatctt cgaagccatt atccaatatt tcgcaggctt
       61 ttgtatattt aggctggtcg atatattcac cgactaaggc attcttagct gttcgagcga
      121 gttcaatatc cgtaaactta aagatcgctt ttactgcttc tctaaacggt tttgaattct
      181 tttttggaat ggaactgaag atgtttctta aaaaatggac ctggcatctc tgccaacttg
      241 cgttggtaaa tgacttacga atcgcagaca ctaggccttt atgggcatca gaaatgatga
      301 gttctgtccc ctttaggccg cgttctttta agtattcaaa gaagatggac catgtgtcat
      361 cactttcttc attttgaatc atgaagccaa tgatttcacg gtcgccacct tctgttatcc
      421 cgatcgcaat atggcagctt ttagaaagca ctcgatggtc ctcacggact tttatgtaga
      481 gaacatcagt catcagataa ggataattcg tacctgagag tgaacggttc tgccattcgt
      541 tgaccatcgg gtctaactgc tcagtcaggc tagaaacaaa agatttcgat acagattttc
      601 cacatagctc ttcaacaatc tttgaaactt tacgtgtcga aacgcccgaa acatacatct
      661 caagcattga agcgagcagt gccttttcat ttcgctgata acgctcaaac accgtcggtg
      721 aaaattcacc atcacgtgtt ctaggcactt ttaattcgag tgtacccaca cgagtcgtaa
      781 agtctcgctc ataatagcca tttctttgac tctgacgact ttctgaacgt tcatagtcat
      841 cggcttgaat atattctgtt cgttgatttt ccatcaattg gttgaaaaca gtggttaaaa
      901 tatttttaga aacatcatct tttaccgaat gttcaataat actttgaacc tcttcgttgt
      961 tcagtgtaaa atgtacttgg gtcatgtaac gtcctcctgg gtatgttttt gtggttaaaa
     1021 acattgtacc gtaaaagggc tgttacatgg ccttttatct tttacacaat tatatggact
     1081 ttatcctata atctgataaa ttattaaggg caaacttatg tgaaagggtg ataactatga
     1141 gcgataaaat acttattgtg gatgatgaac atgaaattgc cgatttggtt gaattatact
     1201 taaaaaacga gaattatacg gttttcaaat actataccgc caaagaagca ttggaatgta
     1261 tagacaagtc tgagattgac cttgccatat tggacatcat gcttcccggc acaagcggcc
     1321 ttactatctg tcaaaaaata agggacaagc acacctatcc gattatcatg ctgaccggga
     1381 aagatacaga ggtagataaa attacagggt taacaatcgg cgcggatgat tatataacga
     1441 agccctttcg cccactggag ttaattgctc gggtaaaggc ccagttgcgc cgatacaaaa
     1501 aattcagtgg agtaaaggag cagaacgaaa atgttatcgt ccactccggc cttgtcatta
     1561 atgttaacac ccatgagtgt tatctgaacg agaagcagtt atcccttact cccaccgagt
     1621 tttcaatact gcgaatcctc tgtgaaaaca aggggaatgt ggttagctcc gagctgctat
     1681 ttcatgagat atggggcgac gaatatttca gcaagagcaa caacaccatc accgtgcata
     1741 tccggcattt gcgcgaaaaa atgaacgaca ccattgataa tccgaaatat ataaaaacgg
     1801 tatggggggt tggttataaa attgaaaaat aaaaaaaacg actattccaa actagaacga
     1861 aaactttaca tgtatatcgt tgcaattgtt gtggtagcaa ttgtattcgt gttgtatatt
     1921 cgttcaatga tccgagggaa acttggggat tggatcttaa gtattttgga aaacaaatat
     1981 gacttaaatc acctggacgc gatgaaatta tatcaatatt ccatacggaa caatatagat
     2041 atctttattt atgtggcgat tgtcattagt attcttattc tatgtcgcgt catgctttca
     2101 aaattcgcaa aatactttga cgagataaat accggcattg atgtacttat tcagaacgaa
     2161 gataaacaaa ttgagctttc tgcggaaatg gatgttatgg aacaaaagct caacacatta
     2221 aaacggactc tggaaaagcg agagcaggat gcaaagctgg ccgaacaaag aaaaaatgac
     2281 gttgttatgt acttggcgca cgatattaaa acgcccctta catccattat cggttatttg
     2341 agcctgcttg acgaggctcc agacatgccg gtagatcaaa aggcaaagta tgtgcatatc
     2401 acgttggaca aagcgtatcg actcgaacag ctaatcgacg agttttttga gattacacgg
     2461 tataacctac aaacgataac gctaacaaaa acgcacatag acctatacta tatgctggtg
     2521 cagatgaccg atgaatttta tcctcagctt tccgcacatg gaaaacaggc ggttattcac
     2581 gcccccgagg atctgaccgt gtccggcgac cctgataaac tcgcgagagt ctttaacaac
     2641 attttgaaaa acgccgctgc atacagtgag gataacagca tcattgacat taccgcgggc
     2701 ctctccgggg atgtggtgtc aatcgaattc aagaacactg gaagcatccc aaaagataag
     2761 ctagctgcca tatttgaaaa gttctatagg ctggacaatg ctcgttcttc cgatacgggt
     2821 ggcgcgggac ttggattggc gattgcaaaa gaaattattg ttcagcatgg agggcagatt
     2881 tacgcggaaa gcaatgataa ctatacgacg tttagggtag agcttccagc gatgccagac
     2941 ttggttgata aaaggaggtc ctaagagatg tatataattt tttaggaaaa tctcaaggtt
     3001 atctttactt tttcttagga aattaacaat ttaatattaa gaaacggctc gttcttacac
     3061 ggtagactta ataccgtaag aacgagccgt tttcgttctt cagagaaaga tttgacaaga
     3121 ttaccattgg catccccgtt ttatttggtg cctttcacag aaagggttgg tcttaattat
     3181 gaataacatc ggcattactg tttatggatg tgagcaggat gaggcagatg cattccatgc
     3241 tctttcgcct cgctttggcg ttatggcaac gataattaac gccaacgtgt cggaatccaa
     3301 cgccaaatcc gcgcctttca atcaatgtat cagtgtggga cataaatcag agatttccgc
     3361 ctctattctt cttgcgctga agagagccgg tgtgaaatat atttctaccc gaagcatcgg
     3421 ctgcaatcat atagatacaa ctgctgctaa gagaatgggc atcactgtcg acaatgtggc
     3481 gtactcgccg gatagcgttg ccgattatac tatgatgcta attcttatgg cagtacgcaa
     3541 cgtaaaatcg attgtgcgct ctgtggaaaa acatgatttc aggttggaca gcgaccgtgg
     3601 caaggtactc agcgacatga cagttggtgt ggtgggaacg ggccagatag gcaaagcggt
     3661 tattgagcgg ctgcgaggat ttggatgtaa agtgttggct tatagtcgca gccgaagtat
     3721 agaggtaaac tatgtaccgt ttgatgagtt gctgcaaaat agcgatatcg ttacgcttca
     3781 tgtgccgctc aatacggata cgcactatat tatcagccac gaacaaatac agagaatgaa
     3841 gcaaggagca tttcttatca atactgggcg cggtccactt gtagatacct atgagttggt
     3901 taaagcatta gaaaacggga aactgggcgg tgccgcattg gatgtattgg aaggagagga
     3961 agagtttttc tactctgatt gcacccaaaa accaattgat aatcaatttt tacttaaact
     4021 tcaaagaatg cctaacgtga taatcacacc gcatacggcc tattataccg agcaagcgtt
     4081 gcgtgatacc gttgaaaaaa ccattaaaaa ctgtttggat tttgaaagga gacaggagca
     4141 tgaatagaat aaaagttgca atactgtttg ggggttgctc agaggagcat gacgtatcgg
     4201 taaaatctgc aatagagata gccgctaaca ttaataaaga aaaatacgag ccgttataca
     4261 ttggaattac gaaatctggt gtatggaaaa tgtgcgaaaa accttgcgcg gaatgggaaa
     4321 acgacaattg ctattcagct gtactctcgc cggataaaaa aatgcacgga ttacttgtta
     4381 aaaagaacca tgaatatgaa atcaaccatg ttgatgtagc attttcagct ttgcatggca
     4441 agtcaggtga agatggatcc atacaaggtc tgtttgaatt gtccggtatc ccttttgtag
     4501 gctgcgatat tcaaagctca gcaatttgta tggacaaatc gttgacatac atcgttgcga
     4561 aaaatgctgg gatagctact cccgcctttt gggttattaa taaagatgat aggccggtgg
     4621 cagctacgtt tacctatcct gtttttgtta agccggcgcg ttcaggctca tccttcggtg
     4681 tgaaaaaagt caatagcgcg gacgaattgg actacgcaat tgaatcggca agacaatatg
     4741 acagcaaaat cttaattgag caggctgttt cgggctgtga ggtcggttgt gcggtattgg
     4801 gaaacagtgc cgcgttagtt gttggcgagg tggaccaaat caggctgcag tacggaatct
     4861 ttcgtattca tcaggaagtc gagccggaaa aaggctctga aaacgcagtt ataaccgttc
     4921 ccgcagacct ttcagcagag gagcgaggac ggatacagga aacggcaaaa aaaatatata
     4981 aagcgctcgg ctgtagaggt ctagcccgtg tggatatgtt tttacaagat aacggccgca
     5041 ttgtactgaa cgaagtcaat actctgcccg gtttcacgtc atacagtcgt tatccccgta
     5101 tgatggccgc tgcaggtatt gcacttcccg aactgattga ccgcttgatc gtattagcgt
     5161 taaaggggtg ataagcatgg aaataggatt tactttttta gatgaaatag tacacggtgt
     5221 tcgttgggac gctaaatatg ccacttggga taatttcacc ggaaaaccgg ttgacggtta
     5281 tgaagtaaat cgcattgtag ggacatacga gttggctgaa tcgcttttga aggcaaaaga
     5341 actggctgct acccaagggt acggattgct tctatgggac ggttaccgtc ctaagcgtgc
     5401 tgtaaactgt tttatgcaat gggctgcaca gccggaaaat aacctgacaa aggaaagtta
     5461 ttatcccaat attgaccgaa ctgagatgat ttcaaaagga tacgtggctt caaaatcaag
     5521 ccatagccgc g
//
LOCUS       DARBET010000121         5220 bp    DNA     linear   BCT 07-NOV-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4027
            SAMEA114569841-rid20112183.denovo.127, whole genome shotgun
            sequence.
ACCESSION   DARBET010000121 DARBET010000000
VERSION     DARBET010000121.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA114569841
            Sequence Read Archive: ERR12203666
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 5220)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5220)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001972618.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001972618.1
            Genome Coverage       :: 132x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/07/2023 13:18:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,041
            CDSs (total)                      :: 2,975
            Genes (coding)                    :: 2,908
            CDSs (with protein)               :: 2,908
            Genes (RNA)                       :: 66
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 67
            CDSs (without protein)            :: 67
            Pseudo Genes (ambiguous residues) :: 0 of 67
            Pseudo Genes (frameshifted)       :: 26 of 67
            Pseudo Genes (incomplete)         :: 28 of 67
            Pseudo Genes (internal stop)      :: 20 of 67
            Pseudo Genes (multiple problems)  :: 6 of 67
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5220
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA114569841-rid20112183.denovo.127"
                     /isolate="VRE4027"
                     /db_xref="taxon:1352"
                     /country="Denmark"
     gene            complement(<1..1256)
                     /locus_tag="SCJ86_002709"
     CDS             complement(<1..1256)
                     /locus_tag="SCJ86_002709"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334404.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I restriction endonuclease subunit R"
                     /protein_id="HEG1170194.1"
                     /translation="MKISHREEAEVEEQLIRVLGEGHNQWTYRPDLKSEEDLWSNLRQ
                     KIISNNQAELNDSPLTDKEFETIKTELLLRTKTPFDAAKWLKGENGMARITIERENPQ
                     LGSVSLILYSNQDIGGGISTYEVVHQIAKRGSNIEARDRRFDVTLLINGLPIIQIELK
                     QVTAKDGFYQAFNQIKKYAEEGMFRNNIFSTLQLFVVSNEQTTRYFANALPKDLHPKF
                     LFSWRTKDNKKVENLYEFCKQVLNIPDAHRLIADYTIVSEDQDNKTLMVLHPYQVHAI
                     QALFIAANKHQSGYVWHATGSGKTLTSFVSTKLLARKSGIDRTIMLVDRKDLDNQTTT
                     EFTKFASEFNTGVSSGNAKANSLIVGTGSAKELSETLLADANANVVIITTRQKLDAAL
                     KYAKKQEEKKGTNRFQKLMGQHIVFVV"
     gene            complement(1270..2487)
                     /locus_tag="SCJ86_002710"
     CDS             complement(1270..2487)
                     /locus_tag="SCJ86_002710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319358.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="restriction endonuclease subunit S"
                     /protein_id="HEG1170195.1"
                     /translation="MSNDTRPEIRFPGFTEDWEERKLGDMMDVTSVKRIHQSDWTNSG
                     IRFLRARDIVSAAKNEEPSDYLYISEEKYNEYSKNSGKVSQGDLLVTGVGSIGVPLLI
                     TDDNPIYFKDGNIIWFKNEHKIDGNFFYYSFINNKIQKYIRDVAGIGTVGTYTIDSGK
                     KTPISLPTYDEQIKIGSFFKQLDHTIMLHQHELDLLKETKKGFLQKMFPKNGAKVPEV
                     RFPGFTGDWEERKLGEVVVFLNGRAFKQQELLDSGKYRVLRVGNFNTNDRWYYSNLEL
                     EEDKYANKGDLLYLWATSFGPEMWNEERVIYHYHIWKLKFLSKELDKQYLYTWLLTDK
                     EKIKESTNGTTMVHVTKGGIEQRTFQFPPDINEQQKIGAFFKQLDDTIALHQRKLDLL
                     KETKKGFLQKMFV"
     gene            complement(2477..4072)
                     /locus_tag="SCJ86_002711"
     CDS             complement(2477..4072)
                     /locus_tag="SCJ86_002711"
                     /EC_number="2.1.1.72"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I restriction-modification system subunit
                     M"
                     /protein_id="HEG1170196.1"
                     /translation="MGAELNQKLFSAADNLRSKMDASEYKNYLLGLIFYKYLSDRLLE
                     QVVLLADESLEEYDTVSKQTMLYRELLSDEESKEDLIATIVDILGYSISPEYLFNVLA
                     DQAKQATFQLNDLNKAFVQLASTYNQFNGLFDDVDLQSKKLGTDEQQRNVTITEVIKK
                     LNDVEVLGHDGDVIGDAYEFLISQFASEAGKKAGEFYTPHMVSDMMAQIVTLDQKERR
                     FFSVFDPTMGSGSLMLNVRNYLTHPDNVKYHGQELNTTTYNLAKMNLILHGVDAEEMN
                     LRNGDTLNKDWPTDEPYTFDAVVMNPPYSANWSADTTFLDDSRFNRYGKLAPKSKADF
                     AFLLHGFYHLKETGTMAIVLPHGVLFRGAAEGVIRQKLLEDGSIYAVIGMPANLFFGT
                     SIPTTVIVLKKNRQTRDVLFIDASREFVKGKNQNKLSEENIQKILETYAERKDVEKYA
                     HLATFDEIKENDYNLNIPRYVDTFEEEEPIDMVHVGNDIKKIRQEQQVLEKELLEAIS
                     SLQTTPENEAWLQGALEVFKHEQ"
     gene            4197..4982
                     /locus_tag="SCJ86_002712"
     CDS             4197..4982
                     /locus_tag="SCJ86_002712"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SinI family restriction endonuclease"
                     /protein_id="HEG1170197.1"
                     /translation="MPNKNRTFESILNEGLTTEPASIIFDEVIDDVEDSKYHKNLKEL
                     FYFALNNGPSLFTGISVPDSDNPLIMAKIYLTKWCNKYIRDRNNPALKKPLKTFGEKD
                     AALTTRVAANANIDDQKVLNDYLRGHFLYMSAENMNGSILEEYLAEVLEPEGWIWCAG
                     SVYRAVDFCYLGTSPILLQVKNKYNTESSSSSAIRVGTTIRKWNRLNKSTKISGLDSP
                     IPNWKALIEMTEASKELAAKLTENSYLAYINEKSTRELWTLDD"
BASE COUNT         1574 a         1051 c          862 g         1733 t
ORIGIN      
        1 actacaaaca cgatatgttg ccccattaat ttttggaagc gattggttcc ttttttctct
       61 tcctgtttct ttgcatattt caacgccgca tctagttttt gacgggttgt aatgatcaca
      121 acattcgcat tggcatctgc caacagagtt tcacttaact ctttggcact tcctgttccc
      181 acgatcaagc tattcgcttt ggcattacca gaagaaaccc cagtattaaa ctccgaagca
      241 aatttcgtaa actctgtggt ggtctgatta tctaaatctt tgcggtccac taacatgatc
      301 gttcgatcga tccccgattt tcgtgctaaa agcttagtag atacaaaact agtcaacgtc
      361 tttcctgaac cagtcgcatg ccacacatac cctgattgat gtttatttgc ggcaataaac
      421 aaggcttgaa tcgcatgcac ttgataagga tgtaacacca tgagggtttt attgtcttga
      481 tcttcactga caatcgtata atccgcaatc aagcgatggg catctgggat atttagcact
      541 tgtttgcaaa actcatataa gttttctacc tttttattat ctttcgttcg ccaactaaat
      601 aaaaacttag gatgcaaatc cttcggtaag gcatttgcaa aataccgagt cgtttgttca
      661 ttggaaacca caaacaactg taaggtcgaa aagatattgt tccgaaacat gccttcttca
      721 gcatattttt tgatttgatt aaacgcctga taaaaaccat cttttgcagt tacttgtttt
      781 agctcgattt ggataatcgg taagccattg atcaataagg tgacgtcaaa acgtcgatcc
      841 cgcgcttcta tgttacttcc acgttttgca atttgatgga ccacttcata agtagagatt
      901 cctccaccaa tgtcttgatt ggaataaagg ataagtgaga cagaacccaa ttgcggattt
      961 tcccgttcaa tcgtgatccg agccatccca ttttcacctt ttaaccattt cgcagcatca
     1021 aatggggttt tcgtccgtag caataattcg gttttgatcg tttcaaactc tttatcggtc
     1081 aaaggagaat cattcagttc tgcttgatta tttgagatga ttttttgacg taaattgctc
     1141 caaagatctt cttctgattt tagatccgga cgatacgtcc actgattatg tccttccccg
     1201 aggacacgaa tcaactgttc ttcgacttcc gcttcctcac ggtgactaat tttcattctt
     1261 cttccctccc taaacaaaca tcttttgcaa gaagcctttt ttcgtttcct tcagcaaatc
     1321 taacttacgt tgatgaagag cgatcgtatc atctagctgc ttgaagaagg caccaatttt
     1381 ttgttgttca tttatatccg gtggaaactg aaatgtcctt tgttctatac cacctttagt
     1441 cacatgtacc attgtcgttc cgttagttga ttctttaatt ttctctttat cagtcaatag
     1501 ccaagtatat agatactgtt tgtccaattc cttagataaa aatttaagtt tccaaatatg
     1561 ataatggtag attacacgtt cttcattcca catttcagga ccgaagcttg ttgcccataa
     1621 atataagaga tctcctttat tagcatactt gtcttcctct aattctaagt ttgaataata
     1681 ccatctatca ttagtattaa aattacctac tcttaaaacc ctatattttc cagaatcaag
     1741 taattcttgt tgtttaaaag ctctaccatt caaaaaaact accacttccc ccaacttccg
     1801 ctcttcccaa tctcccgtaa atcctggaaa acgaacttct ggcacttttg ctccgttttt
     1861 agggaacatt ttttgtaaga atcctttttt cgtttccttc agcaaatcta attcgtgctg
     1921 gtgaagcatg atggtgtgat caagttgttt gaaaaatgaa ccgatcttga tctgttcgtc
     1981 ataagttgga agtgagatag gtgtcttttt accactatca attgtatagg ttccgacggt
     2041 tccaatgcct gcaacgtcac ggatatactt ctgaattttg ttatttatga acgagtagta
     2101 aaagaagttt ccgtcaattt tatgctcatt cttgaaccaa atgatgtttc catccttaaa
     2161 gtaaattggg ttatcatcag tgattaataa ggggacacct attgatccga caccagttac
     2221 aagcaagtct ccttgagaaa ctttacctga atttttcgaa tattcattat atttttcttc
     2281 tgaaatgtaa agataatcgg atggttcctc attttttgca gctgagacaa tatcacgagc
     2341 acgtaaaaat ctaatacctg agttagtcca atctgattga tgaattcgct tcacagaagt
     2401 aacatccatc atatctccta acttacgctc ttcccaatct tccgtaaaac caggaaaccg
     2461 gatttctggt cgtgtatcat tgctcatgtt tgaacacctc cagcgcacct tgtaaccacg
     2521 cttcattttc aggtgttgtt tgcagggaag agatagcttc taaaagttct ttttctagca
     2581 cttgctgttc ttgtcgtatc ttcttgatgt catttccgac atgaaccata tcaattggtt
     2641 cttcttcttc gaaagtatcg acatagcgtg ggatatttaa gttatagtca ttttctttga
     2701 tttcatcaaa ggttgccaaa tgagcgtatt tctcgacatc ttttctttca gcgtaggttt
     2761 cgaggatttt ttgaatgttt tcttccgata atttattctg atttttccct ttaacaaatt
     2821 cacgactagc atcaatgaat aatacatctc gagtttgacg attcttcttc aatacaatca
     2881 ccgtcgttgg aatcgatgta ccaaagaaca agttcgcagg cataccgatc acagcataaa
     2941 tactgccgtc ttctaataat ttttgacgaa tgaccccttc tgcagcacca cggaataaaa
     3001 ccccgtgtgg caagacgata gccatagtcc ctgtttcctt caaatgatag aaaccatgaa
     3061 gaagaaacgc aaaatctgct ttggattttg gcgctaattt cccgtagcga ttaaaccgag
     3121 aatcatctaa gaaggtcgta tctgctgacc aattagccga ataaggtgga ttcatcacta
     3181 ctgcatcaaa tgtgtaaggt tcatctgttg gccagtcctt attcaacgta tccccattgc
     3241 ggagattcat ttcttcggca tctaccccat gtaaaatcaa gttcattttt gctagattgt
     3301 atgtcgttgt atttagttct tgcccatgat atttaacgtt atctggatga gttaagtagt
     3361 tacgtacatt taacatcaag gaacctgaac ccatggttgg gtcaaagaca ctaaagaatc
     3421 gtcgttcttt ttgatccaac gtcacgattt gtgccatcat atcagaaacc atatgaggcg
     3481 tgtaaaattc tccggctttc ttccctgctt ctgaagcaaa ttgactgatc agaaattcat
     3541 acgcatcccc gatcacatcc ccatcgtgac ctaaaacttc cacgtcattc aatttcttga
     3601 taacttcggt aatcgtcaca tttcgttgtt gctcatctgt tcctagtttt tttgattgga
     3661 gatccacatc atcaaataac ccattaaatt gattgtaggt agatgccaat tgcacaaagg
     3721 ctttgtttag atcgttcaat tggaacgttg cttgttttgc ttgatccgct aagacgttga
     3781 aaagatactc tggtgagatc gaatacccca aaatatcaac gattgtggca atcaggtctt
     3841 cttttgattc ttcatctgaa agtaattcac gatacaacat tgtttgtttg cttactgtgt
     3901 catattcctc taaggattca tctgctaaaa gaaccacttg ttccaacaaa cggtcggata
     3961 aatatttata gaaaatcagt cccaataaat aatttttata ttctgacgca tccattttac
     4021 tacggagatt atctgccgca ctaaataatt tttgatttaa ttctgcaccc aaaaagatac
     4081 actcctatct cttatttaaa gttgctatat accctcttat tttagcatac tcccttttag
     4141 aaaaagcaaa aaaaaaagaa aaaatcttat tcttgataac aagctacgtg acgtatatgc
     4201 ctaacaaaaa tagaactttt gaatcaatct taaatgaagg tttaaccaca gaaccggcat
     4261 ccattatttt tgatgaagtt atcgatgacg ttgaagatag taagtatcat aaaaatttaa
     4321 aagaactttt ttattttgca ctcaacaatg gaccaagttt gttcactggt atatcagtac
     4381 ctgatagtga taatccatta attatggcaa aaatttactt aacaaaatgg tgtaataaat
     4441 acatcaggga taggaataac ccagcattaa aaaaacctct taaaacattt ggcgaaaaag
     4501 atgctgcttt aactactaga gtagcagcaa atgctaatat tgatgatcaa aaagtattga
     4561 atgactactt gcgtggacat tttttatata tgagcgctga gaacatgaac ggaagtattt
     4621 tagaggaata tttagccgaa gtgttagaac ctgaaggttg gatttggtgc gccggctctg
     4681 tatatcgagc tgttgatttc tgttatttag gaacctcacc aattctttta caagtgaaaa
     4741 ataaatacaa tacagaaagc agttcgagtt cagctataag agtaggaaca acaattagaa
     4801 aatggaatcg tttaaataag tcgacaaaaa tttctggttt ggatagccct atccccaatt
     4861 ggaaagcatt aatagaaatg actgaagcta gtaaggaact ggcagctaag ttaactgaaa
     4921 atagttattt agcttatatt aatgaaaaat ccacacgaga attatggaca ctcgatgact
     4981 aacaatatcc ttattagtat ctatccacga tacatggaaa aaattataaa caaacaaaaa
     5041 aactttgaat ttagaaattt ctatcttacc tcagatcctt ataatatctt ttggatttac
     5101 gaaacaatcc ccaccaaagc tttaaagtat aaattgattg taaaaaatcc aattactaaa
     5161 cttaagaaaa atcaacacta ctttttagga gacgatgagt ttgaacaatt agtttaaaag
//
LOCUS       DARBET010000122         5061 bp    DNA     linear   BCT 07-NOV-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4027
            SAMEA114569841-rid20112183.denovo.128, whole genome shotgun
            sequence.
ACCESSION   DARBET010000122 DARBET010000000
VERSION     DARBET010000122.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA114569841
            Sequence Read Archive: ERR12203666
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 5061)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5061)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001972618.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001972618.1
            Genome Coverage       :: 132x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/07/2023 13:18:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,041
            CDSs (total)                      :: 2,975
            Genes (coding)                    :: 2,908
            CDSs (with protein)               :: 2,908
            Genes (RNA)                       :: 66
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 67
            CDSs (without protein)            :: 67
            Pseudo Genes (ambiguous residues) :: 0 of 67
            Pseudo Genes (frameshifted)       :: 26 of 67
            Pseudo Genes (incomplete)         :: 28 of 67
            Pseudo Genes (internal stop)      :: 20 of 67
            Pseudo Genes (multiple problems)  :: 6 of 67
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5061
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA114569841-rid20112183.denovo.128"
                     /isolate="VRE4027"
                     /db_xref="taxon:1352"
                     /country="Denmark"
     gene            complement(205..660)
                     /locus_tag="SCJ86_002713"
     CDS             complement(205..660)
                     /locus_tag="SCJ86_002713"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEG1170198.1"
                     /translation="MKKQLFKTFLLSTSILTFGSAITPSGAVFANENNISDIATYSDD
                     EYYYVLDEPVIQTRTWSKPQSWSIAKGSRTYLGIETHSRGTKLSVSANVFGMKIEIAR
                     NTSASGKFKKYRQNARITVTYRVYRKVDNKYIRTQTSTANTQYIDYIPA"
     gene            complement(1063..1236)
                     /locus_tag="SCJ86_002714"
     CDS             complement(1063..1236)
                     /locus_tag="SCJ86_002714"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341717.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEG1170199.1"
                     /translation="MLKKTVLVLGSISLGILGFSPMLLSVIGKNNSNRELKLNSNYYN
                     SPNEELFVKETDK"
     gene            complement(1917..2456)
                     /locus_tag="SCJ86_002715"
     CDS             complement(1917..2456)
                     /locus_tag="SCJ86_002715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016616811.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF536 domain-containing protein"
                     /protein_id="HEG1170200.1"
                     /translation="MADKTIKELAEELGVSKTAINKKVSDKNRKLWFAKNGNKFVINE
                     TGQKAIKSMFLVDNANLNRKLVGEKSKTKNHKNTNQVSELKLLSFLEKQLNRKDIQLQ
                     EKDNQIKQMQKLLDQQQQLTLQANQQIENLQEQLHLTYTEETPTTESTAFSEKKADNT
                     QPLIEKKWWQIWKSNRTNL"
     gene            complement(2472..3395)
                     /locus_tag="SCJ86_002716"
     CDS             complement(2472..3395)
                     /locus_tag="SCJ86_002716"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296860.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation protein"
                     /protein_id="HEG1170201.1"
                     /translation="MANEIVKYENRLNHIPLRKFNSREMNLFFSIASRVREKGVDEII
                     FSFHDLKKLSGYTERGEQFIKDLSSTYDKLLSINAWNDDGNTLTKFVAFTKYSIIRDK
                     QLVIISVNPEFKGLFNQLKNWTRFNLEQFTNLDSTYSKTMFRLLKQYRTQGWTEFSKE
                     KFFDLLDIPKSYWNSPSNIDKFILRPIKEELTPLFKGLTIRKKYGKGRGKPVIGYRFT
                     WKAEINHADDFSKGKQEDLRIKLFNIDHNGELTREEKWRAKDRILNLPLGTHEADFKQ
                     QQQTEEKQAEKRELSNKLKQDLLGNLQNLFD"
     gene            complement(4523..4678)
                     /locus_tag="SCJ86_002717"
     CDS             complement(4523..4678)
                     /locus_tag="SCJ86_002717"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002402449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEG1170202.1"
                     /translation="MKLTKLTISLVIIAGIILYFVLPANQFFLYFGIPTILIVNGVRI
                     FEQRKKM"
     gene            complement(4697..>5061)
                     /locus_tag="SCJ86_002718"
     CDS             complement(4697..>5061)
                     /locus_tag="SCJ86_002718"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322960.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="CPBP family intramembrane metalloprotease"
                     /protein_id="HEG1170203.1"
                     /translation="FSVLFLINILNTSFSEGINPNEVNGSLLLFFLNAATYGAFLEEG
                     IFRFCMIDPQANKKQQYISILISSFLFSIVHGGGLSIFFIGIILSFVYIQTKNIWYSI
                     VAHGFYNTIGILIYLISI"
BASE COUNT         1607 a          839 c          771 g         1844 t
ORIGIN      
        1 aaaaaaaact atatttaaag agttttttta gatttacttc tttgaattca cgaatgaagg
       61 tagaatgaaa tagtataatt attagcacta aaaaaaatat atcactatgt tcaaataaaa
      121 aatatatatt tgacggtact cctaatgatg ggatagcaaa ggtcaaaacc aagtaagcaa
      181 tactaattat ccattttatt gtcattaggc aggaatataa tcaatatatt gtgtatttgc
      241 tgtagaagtt tgagtacgaa tatatttatt atctacctta cgataaactc tgtaggttac
      301 agttattctt gcattttgac gatatttttt aaattttcca cttgcagaag tgtttctggc
      361 tatttcgatt ttcatcccaa atacatttgc actgacagat aactttgtac ctcgactatg
      421 agtttctata cctaaataag tacggcttcc tttagcaatt gaccagcttt gaggtttaga
      481 ccaagtcctt gtttgaataa ctggttcgtc taacacatag tagtactcat catcagaata
      541 agtggctata tcagaaatgt tgttctcatt agcgaatacc gctccgctag gagtaatagc
      601 agaaccaaaa gttaaaatag aagtagataa taaaaaggtt ttaaataact gttttttcat
      661 caaaatcacc tcgaatataa gatacttaag aaaacgcttt ctttatataa ttagaataat
      721 caggaaattt aatgttgtca agagggttta ttttataaaa gctgttatat taaaggttag
      781 tagacataat gtacagtatg ccaagtagcc cttgtatcaa ccgatacagg ggctcacttt
      841 gtgattttta attggtctag gtaaaaagct ctttttaagg cttgattttc tgcagttgct
      901 tctttccgtt tctttgtgaa tttttaccag tcatttttta ctggattgtt atttttcata
      961 aaatatgata aatttttggt agcgctttat aagcgcgttg ctttttcaag aataaacact
     1021 cattattttt taactggctg ctcggaagag cagtcttttt tattatttat ccgtttcttt
     1081 tacaaacaat tcttcgttag gactgttgta ataatttgaa ttcaatttaa gttctctgtt
     1141 ggaattgttt tttccaatta cggataatag catggggcta aaacctaata ttcctagaga
     1201 aatagaaccc agtaccaaca cagttttttt taacataggt tatacctcca ctttttttaa
     1261 cgctaccact tgtatacaca ttttaaatac tagccttcag tttatcaaat gtttaaattg
     1321 aacggatata aatcaaaatt aaattttcaa aagttgttct aaaaaatttt tcttttttgg
     1381 aagttactct aacttttttt gcttgtggtg cttggtatgt tttctccgcc tcccaggttc
     1441 ttgaccattt tctcttataa gtgggatttt taccacgttg agagaaaatg gtcaagaacc
     1501 tgggaggcgg agaatgccct taataccaag caccgcaagc ctttcccata ctttattcga
     1561 tgatcccacg atgatctcat aatgattcta caatgaccct acgatgatca aaattctcaa
     1621 aaaaaataaa cgaaatttca aacgattttt ttgaaatttt gttaaaatca catttgtttt
     1681 tttatgactt tttcaacaaa atcgattaga atttattttg tttttccacc aaaaattgag
     1741 tgagatttca acctatttta agcaataagt caccatatag tttgatagtt tacaactata
     1801 aacattgatt gtttacagtt gtaaactatt ttttctttct cataaaaatt tgagtgatct
     1861 ccccatcagg tagtacaact ttttcctcta ctatccgaac taacttctag aaaattttag
     1921 agattggttc tattagattt ccaaatttgc caccactttt tttcaataag tggttgtgta
     1981 ttgtctgctt ttttttcaga aaaggcagtt gattcggttg ttggagtttc ttctgtataa
     2041 gttaaatgga gctgttcttg taggttctca atttgttggt tcgcttgtag agtgagttgt
     2101 tgttgctggt cgagtaattt ttgcatttgt tttatttgat tatctttttc ttgtagctgt
     2161 atatcttttc gatttaactg tttttctagg aaagaaagga gttttaattc ggaaacttgg
     2221 ttggtgtttt tatggttttt ggtttttgat ttttcgccaa ctagttttcg atttaagttt
     2281 gcgttatcga ctaaaaacat tgattttata gctttttgac cggtttcgtt aatcacaaac
     2341 ttgtttccgt tttttgcaaa ccaaagtttt cgatttttgt cagaaacttt tttgttaatt
     2401 gctgttttac taactcctaa ttcttccgca agttctttga ttgttttatc tgccataaat
     2461 actccttaag cttaatcgaa taagttttgt aaatttccta gtaaatcttg tttgagtttg
     2521 tttgaaagtt ctcgtttttc tgcctgtttt tcttcagttt gttgttgttg tttaaaatca
     2581 gcttcatgag tgcctaaggg aagatttagt attcgatctt tagcgcgcca tttttcttct
     2641 cgtgttaatt ctccattgtg atcaatatta aataatttta tacgtaaatc ttcttgtttc
     2701 cctttggaaa aatcgtcagc atggttaatt tcagctttcc aagtaaaacg ataaccgata
     2761 acaggtttac ctcgaccttt accatatttt ttacgaatag ttaatccttt aaataaggga
     2821 gtgagttctt ctttaattgg ccttaagata aatttgtcaa tatttgacgg actattccaa
     2881 taacttttag gaatatctag taaatcaaaa aatttttctt tggaaaattc agtccagcct
     2941 tgagttcgat attgttttaa taatcgaaac atagtttttg aatatgtgct atccaaatta
     3001 gtaaattgct ctagattaaa tcttgtccag ttctttaatt gattaaaaag tcctttaaat
     3061 tctggattaa ctgaaattat aacaagttgt ttatctcgta taatgctata ttttgtgaaa
     3121 gcaacaaatt tagttaaagt atttccatca tcattccaag cattaatact taataatttg
     3181 tcatacgtgc tactaagatc ttttataaat tgttccccac gttctgtata accgcttagt
     3241 tttttcaaat cgtgaaatga aaaaataatc tcatctacac ctttttcacg tacacgtgaa
     3301 gcaattgaaa aaaataagtt catttcacga ctattaaatt ttctcagagg aatatgattt
     3361 aatctatttt catattttac aatttcattt gccataagct tctcctactt ttataaagta
     3421 tgaaataaaa gtaacatact ttataaaaat actcaataat gtcggaaaac ctacttgaaa
     3481 agcgtagaaa acctacttga aaagcgtaga aaacctactt gaaaagcgta gaaaacctac
     3541 ttgaaaaaac tctgaattgc ttgggagagt aaggacacag ccgacataaa agagatataa
     3601 aaagagatat aaaaagagat ataaaaagag gggaggctaa ttttcccgcc taaaaaatca
     3661 ctcctttttt ctctaaacag gttcgcgaat ttatcaaaac tgccaattcc aaaccttgct
     3721 ccctacggtt cgctaaaaag gtgtgatcgc tctgctctca cattatactg cgcagtcgcc
     3781 tccggctcct ttgctaaaag ccgttcataa ggaacccttt gagaagctct gagcgttcgt
     3841 ttaagacatt ttaagacagt cctagtataa atcccttatc ttgttcttaa aacgccttac
     3901 aggcggtcag ggcaagccct aacaacccta aaattgtcta aatgccctct aaaattcgat
     3961 ttaagcactc ttaaacctat tcttatatat ttatatactt aggcgtttca aacgtcttaa
     4021 aacgcaaatt agagcttgat aaagatattt gctcgtgtgc ctagactttt aaaaaagtcg
     4081 gcacacgcca tgacaaagct gaaataaact gcttcagctt tgtcatggcg gattcgctca
     4141 cttgaaaaag tgtttatttc gctcgattat tagcatatat tttttagctt ttttgctagc
     4201 caaattttgc attaatttgc tcataagtcc caccctttca gtacgtccta cggacgtatc
     4261 gttcgtaatg tgaaagtacc tttaaaggta ctttctacaa aactcactaa aagggtttgg
     4321 caagctctat atttgctagc caaataccta cgtatttggt gcaaataacc gcttgtcaaa
     4381 ggggcgcaag ctcccccttg aatcccccct caaaacccac gctccacaat acaaaaaagg
     4441 ctagcaaatg ctagccactt tttgtaaagt ttcgcttgtt tcaccttcgg tgaaattgtt
     4501 gctttgcaac gatcaggtag agctacattt tttttcgttg ttcaaatatt cttacgccat
     4561 ttactattaa aatcgtaggt attccaaagt aaagaaaaaa ttgattagca ggtaatacaa
     4621 aatataaaat aataccagca ataattacca aacttatagt aagttttgtt aatttcatga
     4681 agtatgccac cttatcttat atagatatca aatagattaa tatgccgatt gtattataaa
     4741 atccatgagc aactatggaa taccatatat tttttgtttg tatataaaca aaacttaaaa
     4801 taataccaat aaagaaaata cttaggcctc ccccatgaac aatagaaaac agaaaagaag
     4861 aaattagtat tgaaatatat tgttgttttt tgtttgcttg aggatcaatc atacaaaatc
     4921 gaaaaattcc ttcttctaaa aaagctccat aagtagcagc atttaaaaag aataaaagga
     4981 gagaaccatt tacttcatta ggatttatac cttctgaaaa agatgtattt agaatattga
     5041 ttaggaataa aacagagaat g
//
LOCUS       DARBET010000123         4788 bp    DNA     linear   BCT 07-NOV-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4027
            SAMEA114569841-rid20112183.denovo.129, whole genome shotgun
            sequence.
ACCESSION   DARBET010000123 DARBET010000000
VERSION     DARBET010000123.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA114569841
            Sequence Read Archive: ERR12203666
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4788)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4788)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001972618.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001972618.1
            Genome Coverage       :: 132x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/07/2023 13:18:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,041
            CDSs (total)                      :: 2,975
            Genes (coding)                    :: 2,908
            CDSs (with protein)               :: 2,908
            Genes (RNA)                       :: 66
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 67
            CDSs (without protein)            :: 67
            Pseudo Genes (ambiguous residues) :: 0 of 67
            Pseudo Genes (frameshifted)       :: 26 of 67
            Pseudo Genes (incomplete)         :: 28 of 67
            Pseudo Genes (internal stop)      :: 20 of 67
            Pseudo Genes (multiple problems)  :: 6 of 67
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4788
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA114569841-rid20112183.denovo.129"
                     /isolate="VRE4027"
                     /db_xref="taxon:1352"
                     /country="Denmark"
     gene            564..989
                     /locus_tag="SCJ86_002719"
     CDS             564..989
                     /locus_tag="SCJ86_002719"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337995.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEG1170204.1"
                     /translation="MICKNKKKIIAIIASFSLIFSTACTVLLTNNANVEAASSSYKHN
                     HAGYRCYKMSYYVSPSKCKQLARQTNKLNTVSAIANFIGLSGLTPGIISLVFGSAVSA
                     NNVFVTAARRGKGVQLNYIAHFSNTTTYNYSSNQNYVIK"
     gene            1019..1183
                     /locus_tag="SCJ86_002720"
     CDS             1019..1183
                     /locus_tag="SCJ86_002720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334262.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEG1170205.1"
                     /translation="MKLTKIAVILAVIAGIILYFVLPANQFFLYFGIPAILIVNGVRI
                     FEQRKKCSST"
     gene            2141..3118
                     /locus_tag="SCJ86_002721"
     CDS             2141..3118
                     /locus_tag="SCJ86_002721"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338009.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation protein"
                     /protein_id="HEG1170206.1"
                     /translation="MANEIVKYHHELNTIPLRKFTSVEMNLFFSIVSRMRDVGDKKVQ
                     FTFEQLKDLSNYKATANVRFIDDLETTYDKLMDLRFGRRSADGLQRERFVLFNQFKID
                     GKADIPFAEIQVHEKALPLLNNLEEWVRYSLQQFNELESSYSKTMFRLLKQYRTKGIA
                     YFSKEDFHELLDIPKSYRESDINKKIIKPIRQELTAIFKGLTIKKKYGKGRGKPVIGY
                     QFTFKPEIKKADDFNKHAPTKEEQNKALELVNQQIYSEKDPTSLQEKIKEQKRKLELL
                     QQLEELERKQNNLNKENKEINEQIPDNNSDIPPELKKGLLDNLQNLFKE"
     gene            3128..3625
                     /locus_tag="SCJ86_002722"
     CDS             3128..3625
                     /locus_tag="SCJ86_002722"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347002.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HEG1170207.1"
                     /translation="MTDKTIKELADILGVSKDKVKYQLRKLPSNFTYKKGNIIYLKKE
                     AVTTITKVLSGNEPGNLPSELPDSLPTDYLLKEIETKNNHIEEKDRQIEKLQLALETQ
                     QKLLDQQQQLTLQANQQIEKLQEQLQLTYTEESSENKSTTLSEETESIEKQSKTEKKW
                     WQFWQ"
     gene            4001..4282
                     /locus_tag="SCJ86_002723"
     CDS             4001..4282
                     /locus_tag="SCJ86_002723"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEG1170208.1"
                     /translation="MDILVRNINPKIITRIDKRCQELTTKTGKKWSRNQYLKILIEND
                     FDHELMNYKKDQFDLLFEKFIAIQAENTNILNDYVHTNNQLIETLIGGI"
     gene            4284..4700
                     /locus_tag="SCJ86_002724"
     CDS             4284..4700
                     /locus_tag="SCJ86_002724"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001232859.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEG1170209.1"
                     /translation="MRKTSVQKHVTLYPSHVDVLDSIIEKNPDVNNYSEAIRFLCMSY
                     EPATNTTTTSNQAKLNAMGKEISIMTEIMAEFADLYLKDTGVLIGTNSKVYQEVKEKI
                     ENQLNPSRRNQPKNRQKETNQTFTSGNFDPTNFLKR"
BASE COUNT         1758 a          817 c          785 g         1428 t
ORIGIN      
        1 aaaaaaaaaa gaaaaatccg cttctgcgaa ttcttctttt ttcattttgg ggcgctgccc
       61 taatccctaa gtcccggtta ttcaatagca tggaaactca aagaataaga cgttccctgg
      121 aataaaaaaa aggctgctcg ttcgagcagt ccatacatag gaaaatgaaa aaacgacgtc
      181 ccttactgag aaacgctatt aaacttttaa caaatcctct aaaaaataac aagtctttaa
      241 aaagtgaccg gtaaaaatgc acaaagaact aaaaaagaga aattgcatca aatcaagcat
      301 tagaagctag taactagcta gaccaattga aaatcacaaa gtgagcgcct gtatctattg
      361 ctacaggcgc tacttggcat agtacacatt atgtcaacaa gaatttaata taacatattt
      421 ttctagtttg ttataacaat gccactctaa caaaaatgta agcattatcc ttattaaaag
      481 ctccttgaca aggactatac cttttatttt atgattttta catagaaaac gttttctcta
      541 ttttttgttt agaggtgatt tttatgattt gcaagaacaa gaaaaaaatt attgctatta
      601 ttgctagttt ctctttgatt tttagtactg catgtactgt cttattaaca aataatgcta
      661 atgttgaagc tgcaagctct tcttacaaac ataatcatgc tggttataga tgttacaaaa
      721 tgagttatta tgtaagtcct tctaaatgca aacaattggc tcgccaaact aataaactta
      781 ataccgtctc agctattgca aactttattg gactatctgg attaacaccg gggattatat
      841 cattagtttt cggatcagca gttagtgcta ataatgtatt tgttacagcc gctagaagag
      901 gaaaaggcgt tcaattaaat tatattgccc attttagtaa taccactacc tataattata
      961 gttctaacca aaattatgtt ataaaataat taaaacttta aactaggagg aatgctttat
     1021 gaaattaaca aaaattgctg taattttagc tgttattgct ggcattattt tatattttgt
     1081 attgcctgct aatcaattct tcctttactt tggaatacct gcgattttaa tagtaaatgg
     1141 cgtaagaatc tttgaacaac gaaaaaaatg tagctctacc taatcgttac aaaacaacaa
     1201 tttcaccttc ggtgaaataa gcgaaacttt acaaaaagtg gctagcaaat gctagccttt
     1261 tttgtattgt agagcgtgaa ttttgagggg gattcaaggg ggagcttgcg cccctttgac
     1321 aagcggttat ttgcaccaaa tacctccgta tttggctagc aatccttcta aaaaatacac
     1381 ctatcgatca gagtcaaaac aaatcaattt tcgagtgagc gaatccgaat ttgcaaagct
     1441 gaagctttgc aaatgagtgt gccggcttcc gtaaaaatac atatggctat aatttgcgtt
     1501 ttaaggcgtt ttaaacgttc tggtatataa ttataccaaa acaactttaa aacgccttag
     1561 atctaatttt agaggtctta tagaacattt tagggttgtt agggcttgcc ctgaccgcct
     1621 gtaaggcgct ttgagaacaa gataagggtt ttatactaag actgtcttaa aacggcttaa
     1681 acaaacgctc agaacttttc aaaggagttc tttatgaact gtttttaagc aaaggagcct
     1741 ttaggcgact gcgcagtata atgtgagagc gtagcgatca cacattttta gtgaccgtag
     1801 ggagcgagtt tcccagttta gaaaaattcg cgaacttgtt tgaggaaaaa agcaggagtt
     1861 ttttaggcgg gaaattttgc ctcctctttt ataatctctt tttaaacctc tttttaaacc
     1921 tctttaccgt cggctgtatg cttactctcc caaggtgtgc agaatctttt gggggtcttt
     1981 ttgtctgttt ttaggggtct ttttgtctgt ttttaggggt ctttttgtct gtttttgggg
     2041 gtctttttgt ctgtttttag gggtctttaa ttaggggctt gacccctatt agaaagctct
     2101 ttataatttt gttaagtagt taatcttgaa ggaggatttt atggcaaatg aaattgtgaa
     2161 ataccaccat gaattaaata cgattccttt acgcaaattt acgtcagtag aaatgaattt
     2221 attcttctct atagtatcta gaatgcgtga cgtaggcgat aaaaaagtac aatttacatt
     2281 cgaacaatta aaagatttaa gcaattataa agcaactgca aatgttcgat tcatagatga
     2341 tttagaaaca acgtatgaca aattaatgga tttacgtttt ggtagaagaa gtgctgacgg
     2401 tttgcaacgt gaacgatttg ttttatttaa tcaatttaaa atcgatggaa aagctgatat
     2461 tccttttgct gaaatacaag tccatgagaa agccttaccc ttacttaata atcttgaaga
     2521 atgggttcga tattctctcc aacaattcaa tgaattagaa agcagttatt ccaaaaccat
     2581 gtttagatta ttaaaacaat acagaaccaa aggaattgcc tatttttcaa aagaagactt
     2641 tcatgaatta cttgatatcc ctaaaagtta tagagaaagt gatataaata aaaaaattat
     2701 aaagcctatt cgccaagaac taaccgcaat cttcaaaggt ctgacaatca aaaagaaata
     2761 cggaaaagga agaggaaaac ctgtcattgg ctatcaattc acgtttaaac ctgaaataaa
     2821 aaaagcagac gactttaata aacacgcacc aacaaaagaa gaacaaaata aagctttaga
     2881 actagttaat caacaaattt actctgaaaa agaccccact tcattacaag aaaaaatcaa
     2941 agaacagaaa cgaaaattag aacttttaca acaattagaa gagctagaaa gaaaacaaaa
     3001 taatctgaat aaagaaaata aggagattaa cgaacaaata ccagataaca attccgatat
     3061 tcccccagaa ttaaaaaaag gcttactaga taatcttcaa aacttattta aagaataagg
     3121 ggtttttatg acagataaaa ctatcaaaga attagcagat attctaggtg tttcaaaaga
     3181 taaagtgaag tatcaactga ggaaattacc tagtaatttt acctacaaaa aaggaaatat
     3241 tatctacctg aaaaaagaag cagtaacaac aattacaaag gttttatcag gtaatgagcc
     3301 gggtaactta cctagtgaat tacctgattc attacctacc gattacctac tcaaggagat
     3361 tgaaactaag aataatcata ttgaagaaaa agataggcaa attgaaaaac ttcaactagc
     3421 tttagaaaca caacaaaaat tactcgacca gcaacaacaa ctcaccctgc aagccaacca
     3481 acaaatcgag aaactgcagg aacaactaca gttgacttat acggaagaat cttcggaaaa
     3541 taaatcaacg actctttctg aagaaacaga atcgatagaa aaacaatcaa agacggagaa
     3601 aaaatggtgg caattctggc aataaaacca gcaatttaaa ttaataaaaa ttgctggttt
     3661 tattcttctt tttgcttttg cgagcagagc aacatgggcc catgttgcga tattttggtg
     3721 gcgccccaat ccccgttttt tcgttgaatc ttactcggac tgcactcgtt ttacagctga
     3781 tttttctgaa ttttgaaaga gcataaggaa acgtggtatc actttcttac gaaaggtgct
     3841 agataagctg tgcctatcac gtttctcaga aaaaaatttg ctatcacttt tttgccattg
     3901 tggctaggct atgtatagtg ttttggcgaa aaatagctat acaaatacta tatttgtatg
     3961 atatacataa attatatact gatacgaaag gaaaatttcc atggacattt tagttcgaaa
     4021 tatcaatccc aaaattatta caagaataga taaacgttgt caggaactaa caaccaaaac
     4081 gggcaaaaaa tggagtcgaa atcaatacct aaaaatttta atcgaaaacg attttgacca
     4141 cgaacttatg aattacaaaa aagatcaatt cgatttatta ttcgaaaaat ttattgccat
     4201 tcaagcagaa aatacgaaca tactaaatga ctatgttcac acaaacaacc aactcattga
     4261 aacacttatc ggaggaatat aacatgagaa aaacttctgt tcaaaaacac gtaacccttt
     4321 atcccagtca cgttgacgtt ttagattcca tcatcgaaaa aaatccagac gtcaacaatt
     4381 attcagaagc cattcgcttt ttatgtatga gttatgaacc tgctaccaat acaaccacaa
     4441 ccagtaacca agcaaaactc aatgccatgg gaaaagaaat ttctatcatg acagaaatca
     4501 tggcagaatt tgctgattta tatctaaaag atactggtgt tcttatcggt acaaattcta
     4561 aagtatatca agaagtgaaa gaaaaaattg aaaatcaact caatccttcc cgacgaaatc
     4621 aacccaaaaa tcgtcaaaaa gaaaccaatc aaacctttac ttctggaaat tttgacccaa
     4681 caaactttct aaaaagataa aaaaagaaac cgaaaaataa aaaaattcct cgatttcttt
     4741 ttagggatcg gtcttccagc aagatttgca agttggtacc aacttgcg
//
LOCUS       DARBET010000124         4775 bp    DNA     linear   BCT 07-NOV-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4027
            SAMEA114569841-rid20112183.denovo.130, whole genome shotgun
            sequence.
ACCESSION   DARBET010000124 DARBET010000000
VERSION     DARBET010000124.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA114569841
            Sequence Read Archive: ERR12203666
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4775)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4775)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001972618.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001972618.1
            Genome Coverage       :: 132x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/07/2023 13:18:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,041
            CDSs (total)                      :: 2,975
            Genes (coding)                    :: 2,908
            CDSs (with protein)               :: 2,908
            Genes (RNA)                       :: 66
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 67
            CDSs (without protein)            :: 67
            Pseudo Genes (ambiguous residues) :: 0 of 67
            Pseudo Genes (frameshifted)       :: 26 of 67
            Pseudo Genes (incomplete)         :: 28 of 67
            Pseudo Genes (internal stop)      :: 20 of 67
            Pseudo Genes (multiple problems)  :: 6 of 67
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4775
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA114569841-rid20112183.denovo.130"
                     /isolate="VRE4027"
                     /db_xref="taxon:1352"
                     /country="Denmark"
     gene            complement(<1..1128)
                     /locus_tag="SCJ86_002725"
     CDS             complement(<1..1128)
                     /locus_tag="SCJ86_002725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288620.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HEG1170210.1"
                     /translation="MKQILRSKMIFDGEQPPFKGYIVINEGIIEKVENNWNYSDLLNE
                     NTELIAYDDQFVMPAIHDNHVFFSGYLAMNAGIDLSDTSSANEAVERIKAVMNHKKAD
                     EPIYAHGWDAALWKTDPSEELLNEINHFGPITAIDKNRSHCWMNAIAKQTYGFTEKET
                     SAESRVLLIKEMLANKKLLAEVYAEFEALLLSKGVISMKEIVFDDAEFLSNIPQRKIL
                     SNLYVQAVQEPLQHELLYEYKEKVFPEKVRFGGVKIMVDGVVADRSGDIYGVYQSGVA
                     SPEIDYEAIKAEVAAFNEVEIPCCLTTEGDKAGYKAASILVEFGKRLPEGVFNSISDL
                     EMVTQKTAEKMNEGQVVAEVYPQILGLNQTLEEAYMSTVIKN"
     gene            complement(1164..1874)
                     /locus_tag="SCJ86_002726"
     CDS             complement(1164..1874)
                     /locus_tag="SCJ86_002726"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288618.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HEG1170211.1"
                     /translation="MLDTNAQLPLYLQLKEILKNKINTGEYPENTKIPAESELIESYE
                     VSRITVRKAVEELVEEGYLLKKQGKGTFVNRHKVFRKIEYIMGFSESCKSNGFTPTSI
                     VLERKIIPATEGMAKKLDIAQGDDVLYIQRKRMADNIPIMLENNYFSYERYSFLMEEE
                     LTDSLYEVLKVQGTLAINPGETALEIVTADDQIAKIMEVAVGTPFFFMDTLINDQNNI
                     PIHVGNQYYLGEVYRFYL"
     gene            complement(1942..2757)
                     /locus_tag="SCJ86_002727"
     CDS             complement(1942..2757)
                     /locus_tag="SCJ86_002727"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301581.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fructoselysine 6-kinase"
                     /protein_id="HEG1170212.1"
                     /translation="MKVLGLGDNVVDKYENLGIMYPGGNSLNFAVFAKKLGHESSFMG
                     VFGSDEEAAHVLSAIKEIGLDNSHSRFEKGENGCARVKIDEGDRIFLGSNSGGVTREY
                     PIQLTEEDREYLNQFELIHMGLYSHVNHLLEELQNVSGKISYDFSDDFTEEEVQRAID
                     KVDFGFFSIEDFSDEEVKQFLMKHFCERNDVLIATRGEKTAIGYDGAKFYEVNPVLEE
                     PVDTMAAGDSFLTAFLLYFLEGDDIVTAMKKGNEFAGKSCLVDGSFGFGIHYD"
     gene            complement(2768..3736)
                     /locus_tag="SCJ86_002728"
     CDS             complement(2768..3736)
                     /locus_tag="SCJ86_002728"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336724.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SIS domain-containing protein"
                     /protein_id="HEG1170213.1"
                     /translation="MDVAKTVNAIVEKNDGEISGIYLVACGGSLVDMYVSDYFLKAEA
                     SKCITGLYTANEFNHVTPKQLDDKSVVILCSHGGNTAETVKAGEVAQARGAVTVGLTH
                     NKTADLLKVSDHSFLYEWGDDAKIVNNPMAIILDLTVSLVNVTETFEHYGKFREGMKV
                     IDTVIENGIKQVQDRIKVFADKYKDETMFYILGSGPSYGHAYGFSICSLMEMQWLDST
                     SVHSGEFFHGPFEVTDKDTNFILLMSIGRTRALDERAKRFLDKYAERVEIVDAKELGL
                     DMISDEVSEFFNPILFYSILSEYRSALADVRNHDLDVRRYMGKVEY"
     gene            4499..>4775
                     /locus_tag="SCJ86_002729"
     CDS             4499..>4775
                     /locus_tag="SCJ86_002729"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_086557923.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase family protein"
                     /protein_id="HEG1170214.1"
                     /translation="MSHTQLIKDTLNILDLNIYFEENCLTKEKYKGQICLIYKGTLLY
                     KPEECSHCLCVVPSRIIRWGTTTVRLLLNDVSEYRTYLELKKQRFKCK"
BASE COUNT         1473 a          983 c          726 g         1593 t
ORIGIN      
        1 attttttata acagttgaca tgtaagcctc ttctaacgtt tgattcaagc cgagaatttg
       61 tggatatacc tctgctacta cttgtccttc gttcattttt tctgctgtct tttgagtaac
      121 catttccaaa tcactaatcg aattaaaaac gccttcaggt aaccgctttc caaattcgac
      181 aagaattgac gcagctttat aacccgcttt atctccttcc gttgtcaaac agcaaggaat
      241 ctctacttca ttgaatgcag caacttctgc tttaattgcc tcatagtcaa tctcgggtga
      301 agctactcct gactgataaa caccgtaaat atctccactt ctatccgcaa caacaccatc
      361 taccatgatc ttcactccac caaagcggac cttttcaggg aaaacttttt ccttatactc
      421 atatagaagt tcatgctgta gaggttcttg gactgcctgc acatataggt tactaagtat
      481 tttcctctga ggtatattag aaagaaattc tgcgtcatca aaaacgattt ctttcattga
      541 tataacacct tttgaaagca gtaatgcttc aaattcagcg taaacctctg ctaaaagctt
      601 tttgtttgct agcatttcct taattaataa tactcgactt tcagcactgg tttctttttc
      661 tgtaaaacca taagtctgct tagcaatagc attcatccaa caatggctgc gatttttgtc
      721 gatcgctgtg attggaccaa agtggtttat ttcattcaat agctcttctg atgggtcagt
      781 tttccacagg gcagcatccc agccatgagc ataaattggt tcatctgctt ttttatggtt
      841 cattacagct ttaatacgct ctacagcctc gttagctgaa gatgtatcgc ttaaatctat
      901 gccagcattc atagccaaat aaccactgaa aaatacatga ttatcatgaa ttgctggcat
      961 aacgaattga tcatcataag caattaattc cgtgttttca tttaatagat cactgtaatt
     1021 ccagttgttt tccacctttt caatgatccc ttcattgatt acaatataac ctttgaaagg
     1081 tggctgctct ccatcaaaaa tcatttttga ccgcaatatt tgcttcataa ataaaatcta
     1141 ctccttgaaa tccaaacagg aaatcataag taaaaacgat atacttctcc caggtaatat
     1201 tgattaccta catgaattgg tatgttgttt tgatcgttga ttaaagtatc cataaagaaa
     1261 aagggcgtcc caacagctac ttccattatt ttggcaattt ggtcatcagc tgtgacaatt
     1321 tcaagtgcag tttccccagg attgatggct aaggtacctt ggactttaag tacttcatac
     1381 aaagaatcag tcagctcttc ctccattaag aaactatatc tttcataaga aaaataattg
     1441 ttttctaaca taatggggat gttatccgcc attcgctttc tctggatata taaaacatca
     1501 tcaccttgag cgatatcgag tttttttgcc atcccttcag ttgctggaat gatcttgcgt
     1561 tccaaaacaa tgctagtcgg tgtaaaacca ttagatttac aactctcact gaaacccata
     1621 atatattcaa ttttacgaaa aactttatgc cggttgacaa aagttccttt cccttgtttt
     1681 ttaagtaggt acccttcttc taccagttct tctactgctt ttctcactgt gattcggctg
     1741 acctcataac tttctatcag ctctgattca gcgggaattt tcgtattctc gggatactcc
     1801 cctgtattga ttttattttt taatatttct tttaattgta agtacaatgg taattgtgca
     1861 tttgtatcca gcattttttt aacctccact ttaccgggat ttctttcttt aggttatcaa
     1921 atgcaggaat ttaaaacaag ctcaatcgta atgaatacca aagccgaacg aaccgtcaac
     1981 cagacaagac ttcccagcaa attcattccc ctttttcatc gcggtgacta tatcgtcccc
     2041 ttcgagaaaa taaagtagga aagctgttaa gaaagaatct cccgctgcca ttgtatcaac
     2101 tggttcttct agcacggggt tcacttcata aaactttgct ccatcataac caatagccgt
     2161 tttttctcct cgggttgcaa tcaatacatc atttcgctca caaaaatgct tcatcaaaaa
     2221 ctgctttact tcttcatcgg aaaagtcttc tatagaaaag aagccaaaat caactttatc
     2281 aattgcccgc tgaacctctt cttccgtaaa gtcgtcagaa aaatcataag aaatctttcc
     2341 agaaacattt tgtaactctt ctaatagatg attgacatgg ctatataatc ccatatgaat
     2401 caattcaaac tggtttaaat attctctgtc ttcttcagta agctgaatcg ggtattcacg
     2461 agtaacacct cccgaattgc tgccaaggaa aatccggtcg ccctcatcaa tcttcactct
     2521 ggcacagcca ttctcgcctt tttcaaagcg agagtgactg ttgtccagtc ctatttcttt
     2581 tattgctgat aaaacatgag ctgcctcctc atctgagcca aaaactccca taaagctact
     2641 ttcatgtccc agtttttttg caaaaactgc aaaattcaat gaatttcctc caggatacat
     2701 aattcccaga ttttcatatt tatcgacgac gttatcacct aagcctaata ccttcatttt
     2761 aatcctccta gtactccact ttccccatat agcgacgaac atctaaatca tgattgcgta
     2821 catctgctaa agctgaacga tattctgaaa gaatactgta gaataaaatg ggattgaaga
     2881 attcagaaac ttcatctgat atcatatcca atcctagttc ctttgcatca acgatttcta
     2941 ctctttctgc atatttatca aggaatcgtt ttgcccgttc atctaaagca cgagtgcgtc
     3001 caatgctcat caagagaata aaattcgtat ctttgtctgt tacttcaaaa ggtccatgga
     3061 agaattctcc agaatgaaca gaagttgaat caagccattg catttccatc aaagagcaaa
     3121 ttgaaaaacc ataagcatgt ccatatgatg gtccactacc taagatataa aacattgttt
     3181 catctttgta cttatcagca aacactttaa tacgatcctg cacctgctta atgccatttt
     3241 caattacggt atcgataacc ttcatgcctt ctctgaattt accgtaatgt tcaaatgttt
     3301 cggtaacatt gaccaaagat acagttaaat caagaatgat ggccatagga ttattgacta
     3361 tttttgcatc atctccccat tcgtaaagga aactatgatc agaaaccttt aataaatccg
     3421 cagttttatt atgagtcaga ccaacagtca cagctcctct agcttgagcg acttctcctg
     3481 cttttaccgt ttctgctgta tttcctccat gagaacatag aatgacaaca gatttatcat
     3541 ctaattgctt tggggttaca tggttaaatt cgttcgctgt atataagcct gtaatacact
     3601 tcgatgcttc ggctttaaga aaataatctg agacatacat atctaccagc gaaccgccac
     3661 aagccaccaa ataaattcca gaaatctctc catcattttt ttctacaatt gcgttaaccg
     3721 tttttgctac atccatcaac atttcctcct taaaatttta aaaatgttaa ttttatccaa
     3781 aataaaagta actgattaaa tgtaaagata tcacataacg tcatataacg tcatgtgaca
     3841 taattgcatt gtatccgctt tctttgtgtt tgtcaatagg aaaaatttat ttctctaaaa
     3901 aatataaata atctgcagtg aaacttgagt acatatacta atttcgaaaa atgcctatga
     3961 tttaaataat tatggtgctg ggatgaaggt ttataagttt gtcttgattt gtttagaaga
     4021 aatcgttttt gcacacagtt gaattaggaa actactggag tggaaaataa agtgatttga
     4081 cgggtagatg ggtgcactta aatttgaaaa tggcttaaaa tgctttattt tgaattgcag
     4141 aatttctttt agtacgcaat ttatggtctt aaaactgatt aaaaagtgat acattgattt
     4201 taatacaatt tatgatacaa aaaatgacct cctttcttaa ataaaagagg tcattttgtt
     4261 ttgtctcata atcgattgtt tttgatacag tagttatatt cagaaaaaat aaagactctt
     4321 ctgagtttat aatcctttgg attatatacg gatacagatg tagacataaa acaagctcaa
     4381 ttttcaaaaa tggcattact cacctttttg gtgaattatt tgtataaaaa aaatatagaa
     4441 aagacaccct gaaatccttt agaattaatt tggctaccaa ttcataggag gattcaagat
     4501 gtctcatact cagcttatca aagataccct aaatattcta gacctaaata tctatttcga
     4561 ggaaaattgt ttaactaaag aaaaatacaa agggcaaatt tgcttgattt acaaaggaac
     4621 tcttctgtac aagccagaag agtgttccca ttgtctttgt gtcgtcccct ctaggatcat
     4681 tcgttggggg acgacaaccg ttcggttgtt actgaatgat gtgtctgagt atcgcactta
     4741 tctggagctg aagaaacaac gatttaaatg taagt
//
LOCUS       DARBET010000125         4720 bp    DNA     linear   BCT 07-NOV-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4027
            SAMEA114569841-rid20112183.denovo.131, whole genome shotgun
            sequence.
ACCESSION   DARBET010000125 DARBET010000000
VERSION     DARBET010000125.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA114569841
            Sequence Read Archive: ERR12203666
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4720)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4720)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001972618.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001972618.1
            Genome Coverage       :: 132x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/07/2023 13:18:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,041
            CDSs (total)                      :: 2,975
            Genes (coding)                    :: 2,908
            CDSs (with protein)               :: 2,908
            Genes (RNA)                       :: 66
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 67
            CDSs (without protein)            :: 67
            Pseudo Genes (ambiguous residues) :: 0 of 67
            Pseudo Genes (frameshifted)       :: 26 of 67
            Pseudo Genes (incomplete)         :: 28 of 67
            Pseudo Genes (internal stop)      :: 20 of 67
            Pseudo Genes (multiple problems)  :: 6 of 67
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4720
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA114569841-rid20112183.denovo.131"
                     /isolate="VRE4027"
                     /db_xref="taxon:1352"
                     /country="Denmark"
     gene            complement(<1..1097)
                     /locus_tag="SCJ86_002730"
     CDS             complement(<1..1097)
                     /locus_tag="SCJ86_002730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014748779.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS256 family transposase"
                     /protein_id="HEG1170215.1"
                     /translation="MARKKRNPDAEKLAESILNTYQPESVEDMQDVLKDVFGPLFEKM
                     LQGELNNHLGYDAHSKEPKKHDNRRNGYGNKTLKTSFGKVNIDVPRDREASFEPELIP
                     NRKRDVSDIEGKVLSMYARGMSQCDIAATVEDIYGFDISHEMISDITDAVLPELEEWQ
                     ARPLAKCYAFLFVDCMYVTLRENYEAKEYAVYTILGYDLKGNKEILGLWLNQTESKNR
                     WMQVFDELKVGGVEDVFFISMDGVSGLKEGAKAIFPSVIVQRCIVHLVRNALRYLPSK
                     DYKEVCRDMKKFYSASSLNAAHAAFDSFQNRWSHYSGAVDVCKRNFSHVEQLFDYGSA
                     IRKIMYTTNAVESIHSSFRKVTKKGVFSNENA"
     gene            complement(1227..2603)
                     /locus_tag="SCJ86_002731"
     CDS             complement(1227..2603)
                     /locus_tag="SCJ86_002731"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017647836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEG1170216.1"
                     /translation="MIKNAFAYVTRKSLKSLIIILVILSMSTLSLISLSIKDATDRAS
                     KETFANITNSFSMEINRQVNPGTPRGGGNVKGEDIKKISQTDSIDSYVKRINSVADLV
                     DYDIIETQETLANQSPERAKNFKRTVMLTGVNDSTKETKFVSEAYKLVEGKHLENEDK
                     NKILMHKDLAEKNNLKVGDKIKIKSNLFDADNEKGADETVEVEIKGLFDGHNSGGVSA
                     AQELYENTLITDIHTAAKVYGNTEDTAVYQDATFFVKGDKNLDSVIKDLGKLDINWRE
                     YNLIKSSSNYPALQQSISGIYSISNKLFFGSLIFAGVVVSLLLFLWMNARKKEIAVLL
                     SLGISKLEIFGQFLIEMVFISIPAFVGSYFLAQYTADKLGNNILNKVTGDIAKQIAKQ
                     SASSQLGGGAEAEGFNKTLSSLDINVLPKFMIYVVIFMSLVLLVSLIISSFNILRRNP
                     KELLIDNN"
     gene            complement(2603..3259)
                     /locus_tag="SCJ86_002732"
     CDS             complement(2603..3259)
                     /locus_tag="SCJ86_002732"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004612033.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEG1170217.1"
                     /translation="MDILEIKNVAYSYANSKEKVLSGVNQKFELGKFYAIVGKSGTGK
                     STLLSLLAGLDKPQTGKILFKNEDIQNKGYSNHRKNNISLVFQNYNLIDYLSPIENIR
                     LVNKSADESILFELGLDKKQIKRNVMKLSGGQQQRVAIARALVSDAPIILADEPTGNL
                     DSVTAGEIINILKTLAKDRNKCVIVVTHSKEVADSADIILELSGKKLKKVNKMNLEVE
                     "
     gene            complement(3269..4546)
                     /locus_tag="SCJ86_002733"
     CDS             complement(3269..4546)
                     /locus_tag="SCJ86_002733"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009898531.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEG1170218.1"
                     /translation="MIKNAIAYITRKKNRTLIIFIILTIVLSCLYSCLTIMKSSNEIE
                     KALYESSNSSISITKKDGKYFNVNQFKDIEKLKEIEEKIIQYDGLAKLKDAKVVSGEQ
                     RINREDLSDEFKNVVSLEATNNTKRNILFSSGVFTIKEGKNIEENDKNSIIVHEEFAK
                     QNNLKLGDEVDLELLDIEKSGKIKSHKFKIIGIFSGKKQETYTGLSSDFSENMVFVDY
                     STSQEILNNSENNEIANKILMYSGSAESTDLALNKLKELKIDESKYFVEKDSNAFEES
                     LESVSGIKYIIKVMTYSIMLGGMVVLSLILILWLRERIYEIGIFLSIGTSKIQIIMQF
                     IFELIFISIPSIISSLFLGNVLLKVIVDGFINSEDSMISGGSLINNSSFMSNITTLGQ
                     SYLILISIIVLSVVFASSLMLIKKPKEILSKIS"
     gene            complement(4565..>4720)
                     /locus_tag="SCJ86_002734"
     CDS             complement(4565..>4720)
                     /locus_tag="SCJ86_002734"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008392734.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S binding protein"
                     /protein_id="HEG1170219.1"
                     /translation="DKDKCINCGKCKKVCQMNKDSVENCNHLECIRCGRCKNACPVDA
                     ISYGVRK"
BASE COUNT         1477 a          858 c          566 g         1819 t
ORIGIN      
        1 gcattctcat tagagaacac tccctttttt gtgacttttc tgaagctaga gtgaatactt
       61 tcaactgcat tagtagtgta catgattttt ctaatggcgc tgccataatc aaagagttgt
      121 tcaacatgag aaaagtttcg tttgcataca tctactgcac cagaataatg agaccaccga
      181 ttttgaaaac tgtcgaaggc agcgtgtgca gcgtttaaag aagaagcact gtagaatttt
      241 ttcatgtctc gacagacttc cttataatcc ttacttggga ggtatcgtaa cgcatttcga
      301 acaagatgaa caatacagcg ctgcacaatg acagaaggaa aaattgcttt tgcgccttct
      361 ttgagaccgg aaacaccgtc catcgaaatg aaaaaaacat cttctacacc acctactttc
      421 agttcatcaa atacttgcat ccagcgattt ttagattctg tttgattgag ccataggcct
      481 aaaatctcct tgtttccttt aagatcatag ccaagaatgg tgtacacagc atattcttta
      541 gcttcatagt tttcgcgtaa agtgacgtac atacaatcaa cgaatagaaa tgcataacac
      601 ttggctaatg gccgagcttg ccattcttcc aattcaggaa ggacggcatc cgtgatatca
      661 gaaatcattt cgtgggaaat atcaaagccg taaatatctt caacggttgc ggcgatatcg
      721 cattgactca ttcctcgtgc atacatggaa agaaccttac cttcgatgtc ggagacatct
      781 cgttttctat taggaatcaa ctcaggttca aaagaagcct cccggtctct gggaacatca
      841 atgtttactt taccaaagct cgttttaagc gtcttattgc cataaccgtt tcgtcgattg
      901 tcatgtttct ttggctcttt agaatgggcg tcatacccta aatggttatt caattctcct
      961 tgaagcattt tttcaaataa gggaccaaac acatctttta aaacatcttg catatcctct
     1021 acagattcgg gttgataagt attcaaaatg gattcagcta acttctcagc atcaggattt
     1081 cttttctttc tagccatcgt gtaatcacct cttgatctta ttttagaaaa ataaaaaccg
     1141 tgaagcaacc acccgcctag gattgatggt tacttacacg gtttatatta cactctcatt
     1201 tttggtgcta aaaagcaccg aaaattttaa ttattgtcaa ttaataattc ttttggattc
     1261 cttcttaata tattgaatga agaaataatc aatgatacaa gaagtactaa actcataaat
     1321 ataactacat aaatcatgaa tttaggtaat acattgatat ctaaacttga taatgtcttg
     1381 ttaaatcctt cagcttctgc cccaccacct aattggctag atgcagattg tttagctatt
     1441 tgtttagcta tatcaccagt aaccttattc aatatattat tcccaagctt gtcagctgta
     1501 tattgagcta agaaatagga accaacgaat gcggggattg atataaatac catctcaatt
     1561 aggaattgac caaaaatttc taattttgat atacctaatg atagtaatac tgctatttct
     1621 ttctttctag cattcatcca taagaataat aataatgaaa ctacaactcc tgcaaatatt
     1681 aatgagccaa aaaataattt atttgaaatt gagtaaatac ctgatataga ttgttgtaat
     1741 gcagggtaat ttgatgaact tttaatcaaa ttatattctc tccaatttat atctaatttc
     1801 ccaagatctt ttattacact atcaagattc ttatcacctt ttacaaagaa tgttgcatct
     1861 tggtatacag ctgtatcttc ggtattacca taaactttag cagctgtatg aatatcagtg
     1921 attaatgtat tttcgtatag ttcttgtgct gcacttactc caccgctatt atgcccatcg
     1981 aatagacctt taatttctac ttctactgtt tcatctgcac ctttctcatt atcagcatca
     2041 aataaattag attttatttt tatcttatct ccaactttaa ggttattttt ttcagctaaa
     2101 tctttatgca ttaagatttt atttttatct tcattttcta aatgctttcc ttctactaat
     2161 ttatatgctt ctgatacaaa ttttgtttct tttgttgagt cgttaacccc agttaacata
     2221 actgttcttt taaaattctt tgctctttca ggtgattgat tcgcaagagt ctcttgagtt
     2281 tcaataatat cataatcaac taaatctgca acactgttta ttctttttac ataggagtct
     2341 atactatctg tttgagatat ctttttaata tcttcacctt ttacatttcc cccacctcta
     2401 ggtgttcctg gatttacttg tctatttatc tccatagaaa aactatttgt tatattagca
     2461 aatgtttctt ttgaagctct gtccgtagca tctttaatag ataaactaat gagacttaaa
     2521 gttgacatag ataaaataac taatatgata attaatgatt ttaaactttt tctagttaca
     2581 taagcaaatg cattttttat cattattcaa cctccaaatt cattttattt acttttttca
     2641 actttttacc acttagttct aaaatgatat ccgcagaatc tgctacttcc ttactatgtg
     2701 ttacaactat tacacattta tttctatctt tagctaatgt ctttaatata ttaattattt
     2761 ctccagcagt aacactgtct agattaccag taggctcatc agctagtatt attggagcat
     2821 ctgataccaa tgctctagca atagctactc tttgttgctg tccaccagat aatttcataa
     2881 catttctttt tatttgtttt ttatctaaac ctagttcaaa caagatactt tcatctgctg
     2941 atttatttac taatctaata ttttcaattg gtgataaata atctattaaa ttatagtttt
     3001 gaaatactaa agatatatta ttttttctat gattactata tcctttattt tgtatatctt
     3061 cattcttaaa caatattttt cctgtttgag gtttatcaag tccagcaagt aaggaaagaa
     3121 gtgtagattt tcctgttcct gactttccta ctatcgcata aaacttccca agttcaaatt
     3181 tttgatttac tcctgacaaa actttttctt tagaatttgc ataactatat gctacattct
     3241 ttatttctaa tatatccatt attttctcct aacttatttt tgataagatt tctttaggct
     3301 tcttgattaa cattaatgaa gaagcaaata caactgataa aacaataata cttattaata
     3361 ttaaataact ttgtccaagt gttgttatat ttgacataaa actactatta tttattaaac
     3421 ttccaccgga aatcattgaa tcctctgagt taatgaatcc atctacaatt acttttagta
     3481 atacatttcc taaaaataag gaagatatta tacttggtat cgatatgaat attaactcga
     3541 atataaattg cattataatt tgtatcttag atgttccaat agataagaat atacctattt
     3601 cataaattct ttcccttaac catagaatca agattaatga aagaacaacc atcccaccta
     3661 acataataga ataagtcatt acttttatta tatattttat tccactcact gactctaaag
     3721 actcttcaaa cgcattagaa tctttttcaa caaaatactt tgactcatca attttaagct
     3781 ctttcaattt gtttaaggct aagtctgtag attctgcact accagaatac attaaaattt
     3841 tatttgcaat ttcattattt tctgaattat ttaatatctc ttggcttgtt gaataatcta
     3901 caaacaccat attttcacta aaatcagaag ataatcctgt atatgtttcc tgttttttac
     3961 cagaaaagat ccctataatt ttaaatttat gactttttat ttttccactt ttttcaatat
     4021 ctagtaattc aagatcaact tcatcaccta attttagatt gttttgttta gcaaattctt
     4081 catgaacaat aattgaattc ttatcatttt cttctatatt tttaccttct ttaattgtaa
     4141 atactccact actaaataaa atatttcttt tagtattatt tgtagcttcg agtgaaacaa
     4201 cattcttaaa ttcgtcagat aaatcttctc tatttattct ttgttctccg ctaactactt
     4261 tagcatcttt tagttttgct aatccatcat attgaattat tttttcttca atttctttta
     4321 atttttcaat atctttaaat tgattaacat taaaatattt accatctttt tttgttattg
     4381 atattgaaga attagaactt tcatataaag ccttttctat ttcattgctt gatttcatta
     4441 ttgttaaaca agaatacaag caagaaagaa ctatagttaa tatgataaaa attattaaag
     4501 ttctattttt ctttcttgta atatatgcta tagcattttt tatcactttc ctaactactc
     4561 ctctctattt tcttactcca taactaattg catccactgg acaagcattt ttacatcgtc
     4621 cacatctgat acactccaga tgattgcaat tctcaactga atctttattc atttggcaaa
     4681 cttttttaca ttttccacaa ttaatacact tatccttatc
//
LOCUS       DARBET010000126         4647 bp    DNA     linear   BCT 07-NOV-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4027
            SAMEA114569841-rid20112183.denovo.132, whole genome shotgun
            sequence.
ACCESSION   DARBET010000126 DARBET010000000
VERSION     DARBET010000126.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA114569841
            Sequence Read Archive: ERR12203666
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4647)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4647)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001972618.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001972618.1
            Genome Coverage       :: 132x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/07/2023 13:18:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,041
            CDSs (total)                      :: 2,975
            Genes (coding)                    :: 2,908
            CDSs (with protein)               :: 2,908
            Genes (RNA)                       :: 66
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 67
            CDSs (without protein)            :: 67
            Pseudo Genes (ambiguous residues) :: 0 of 67
            Pseudo Genes (frameshifted)       :: 26 of 67
            Pseudo Genes (incomplete)         :: 28 of 67
            Pseudo Genes (internal stop)      :: 20 of 67
            Pseudo Genes (multiple problems)  :: 6 of 67
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4647
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA114569841-rid20112183.denovo.132"
                     /isolate="VRE4027"
                     /db_xref="taxon:1352"
                     /country="Denmark"
     gene            complement(134..718)
                     /locus_tag="SCJ86_002735"
     CDS             complement(134..718)
                     /locus_tag="SCJ86_002735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007207927.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HEG1170220.1"
                     /translation="MKIGYARVSTGLQNLDLQEDSLNKFGCEKIFTDHMSGAKSNRPG
                     LEMAIDFVRSGDTLVVWRLDRLGRNMEDLISIVNRLNERGVSFHSIQENITMDKSSST
                     GQLMFHLFAAFAEFERNLILERSAAGRAAARARGRFGGRPEKLSEQDLELLKTLVDSG
                     TPIKTIAERWNVSRTTIYRYLAKMEEKNGKTNSH"
     gene            complement(929..1132)
                     /locus_tag="SCJ86_002736"
     CDS             complement(929..1132)
                     /locus_tag="SCJ86_002736"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEG1170221.1"
                     /translation="MPEFVNTPNFYQDSDILSKINLPKEESALQVQRTLQETQKIQKS
                     ISNLEDSEEKRKKRDQKTYQKEK"
     gene            complement(1142..1564)
                     /locus_tag="SCJ86_002737"
     CDS             complement(1142..1564)
                     /locus_tag="SCJ86_002737"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303116.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEG1170222.1"
                     /translation="MKNKFILSLGILTIGISVTGTTIPTFADSTNEPTTVVDNNQELT
                     PLDRDGIAIGNPTNDLTVTENYLIVSPLLALAASKTIRVTGTMIFDKVGWPSRPPQSR
                     NWKEKRNGYWYYGKLYLQSYKSAGNNWIATYSGTLTRK"
     gene            1802..2416
                     /locus_tag="SCJ86_002738"
     CDS             1802..2416
                     /locus_tag="SCJ86_002738"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001261742.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HEG1170223.1"
                     /translation="MRVAYIRVSSIDQNEQRQIEEMKKFGAERIFIEKQSGATITHRP
                     VFQEALDYVRDQDIFIVEAIDRLGRNYDEIIASVNYLKKKNVKLIITSLPIMAEAIGN
                     PLLDKFIKDLIIQILAMIAEQERTESKRRQAQGIKIAKANGVYKGRPKLYSADAKDPQ
                     RRLVYKSIVEDLKNGVAIAKIAKDYNVTRQTVYRIKKDSMVNDK"
     gene            complement(2581..3405)
                     /locus_tag="SCJ86_002739"
     CDS             complement(2581..3405)
                     /locus_tag="SCJ86_002739"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001809248.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEG1170224.1"
                     /translation="MIERLKDFMISVWDVAMLADESNYTSSIYRLEDSNWIEISLELK
                     TLPNSVNYIIRDITSEAQQLVNYMHGHESIPFSLILLKRSKNISNTRDRFISIITACE
                     IGVKEFYQQEKPDLTLIFENLQSPPVDKLLGSMFKGYFNIEFPKEIRKEIKNFIEKRN
                     KLVHSPQGSKPTIEECFECYMAVLKTFNFFYKINDSFMYRDFYEESIELTPTEPNKSQ
                     ITLSKELERKVESQELAIGISITNVSTYKELYEAKSQDNKDFVENIDKEPTNTVGD"
     gene            complement(3429..3584)
                     /locus_tag="SCJ86_002740"
     CDS             complement(3429..3584)
                     /locus_tag="SCJ86_002740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347142.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEG1170225.1"
                     /translation="MEYKFNLNLKEVLPTQGTININGQDNDDDIVIKKIEFIDSDINV
                     FFILKKN"
     gene            complement(4043..4300)
                     /locus_tag="SCJ86_002741"
     CDS             complement(4043..4300)
                     /locus_tag="SCJ86_002741"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016632526.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Txe/YoeB family addiction module toxin"
                     /protein_id="HEG1170226.1"
                     /translation="MIKAWSDDAWDDYLYWHEQGNKSNIKKINKLIKDIDRSPFAGLG
                     KPEPLKHDLSGKWSRRITDEHRLIYRVENETIFIYSAKDHY"
     gene            complement(4293..4562)
                     /locus_tag="SCJ86_002742"
     CDS             complement(4293..4562)
                     /locus_tag="SCJ86_002742"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016632525.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system Phd/YefM family
                     antitoxin"
                     /protein_id="HEG1170227.1"
                     /translation="MEAVAYSNFRQNLRSYMKQVNEDAETLIVTSKDVEDTVVVLSKR
                     DYDSMQETLRTLSNNYVMEKIRRGDEQFSKGAFKTHDLIEVESDD"
BASE COUNT         1533 a          816 c          626 g         1672 t
ORIGIN      
        1 ttaatattaa caaatgaata aaatccttac aacatttgag aataagttat cagtagacac
       61 ttacaaaaaa ttctcatact attgtcactt ttctagataa atggcaaaaa ttttcagcat
      121 taaatgacaa aaactaatga gaatttgtct ttccattttt ctcctccatt tttgccaaat
      181 aacgataaat ggtggttctt gagacgttcc accgttccgc aatagtctta ataggtgtac
      241 cgctatccac taaagtcttc agtaattcca aatcttgttc agacaatttt tcaggacgac
      301 caccaaaccg tcctcttgca cgagcagctg ctcgtcctgc tgcggatcgt tccaggatca
      361 aattacgctc aaattctgca aatgccgcaa acaaatgaaa catgagctgt cctgtagaac
      421 ttgatttgtc catcgtaata ttttcttgaa tactatgaaa actcacacca cgctcattca
      481 gtcgattgac gattgaaatc aaatcttcca tatttctacc taaacgatct aagcgccaaa
      541 cgacaagtgt atcgccagag cgaacaaaat caatggccat ttctaatcct ggtcgattac
      601 tttttgcccc actcatatga tcagtaaaaa ttttttcaca accaaattta tttaggctgt
      661 cttcttgtaa atccaaattt tgtaaaccag tagatacccg ggcgtatcct attttcaaaa
      721 aaatcactcc ttcattaatt ttattgtaac ataactttat ccatatagtt taactgaaca
      781 tagatattgt tatgggttta tgaacatgaa atgaattgtt tttttgaaaa ataaagcaaa
      841 aaaagaaagt gtccagaaat gggtgatttt atgaacactt tcaattagaa aaaaagaatg
      901 tcgactagac actctttttt taaaatcatt atttttcttt ttgatatgtt ttttggtctc
      961 ttttttttct tttttcttca gaatcttcta gattagaaat tgatttttga attttttgtg
     1021 tctcctgtaa agttcgctga acttgtaaag ctgattcttc tttcggtaaa ttaattttac
     1081 ttagaatatc gctatcttga taaaaatttg gtgtgttcac aaattctggc ataaaaaatc
     1141 cctactttct agttaaggtt cctgaataag tcgcaatcca attgtttcca gctgatttat
     1201 aactttgtaa atacaatttt ccatagtacc aataaccatt tcttttttct ttccaattcc
     1261 gtgattgagg aggtctagaa ggccaaccaa ctttatcaaa aatcatcgtt cctgtcactc
     1321 gtatcgtttt agaagctgct agagcaagta atggagaaac tattaaataa ttttcagtaa
     1381 cagttaagtc gttagttggg ttaccaatgg ctattccgtc cctatctaaa ggagttaatt
     1441 cttggttatt atctactaca gtagttggtt catttgtact atcagcaaat gtaggaattg
     1501 tagttccagt tacactaatt ccaatagtta aaatacctaa agataaaata aacttatttt
     1561 tcattgtcta tcacctcgaa atcaaaacta agaaaacgtt ttcttaataa atcttaacag
     1621 ataaatcaag ttatatacta gaaaattcat aaaatatatt tttaatgtca taatcgttta
     1681 aattttcttg attgacattt atttacaaaa aatattgcca tttaaaaaga aaagtgacat
     1741 atggcttcct cgtgtatgat tagggtgtat tttaaatagt tgcagaaagt aggtaaaagt
     1801 tttgagagtt gcatacatac gtgtctcatc gatcgatcaa aatgaacaac gccagattga
     1861 agaaatgaaa aaattcggtg cagagagaat tttcatcgag aaacaatctg gcgcaacgat
     1921 tactcatcga cctgttttcc aagaagcatt agattacgtc cgagatcaag atatttttat
     1981 tgtggaagct attgatcgac ttggaagaaa ttatgacgag atcatcgctt cagtcaatta
     2041 cttgaagaag aaaaatgtga agctgatcat tactagttta ccgatcatgg ctgaagcaat
     2101 tggcaatcct ctcctggata aattcattaa ggatctgatc atccaaattt tggccatgat
     2161 cgctgaacaa gaacggactg aatcgaaacg tcgccaggca caaggaatta agatcgcaaa
     2221 agcaaacggc gtctacaaag ggcggcccaa attatacagt gcagacgcaa aagatcccca
     2281 acgtcgcctt gtttacaaaa gcatcgttga agatcttaaa aatggtgttg cgattgctaa
     2341 gattgctaaa gactacaatg ttacgagaca aaccgtgtat agaataaaaa aagacagcat
     2401 ggtcaatgac aaatagttga tcatgctgct tttattatct tctcatcttt aatttcctac
     2461 atataattac tatctataaa attaagtctt actcagaaca gataaagcat aaaattccaa
     2521 caaaccattt tttacttaac taaataaaga gtctgttaat ataatcttca cttatttacg
     2581 ttaatcccct actgtatttg ttggttcctt atctatattt tctacaaaat ccttattatc
     2641 ttgtgattta gcttcgtata attctttgta tgtcgacaca ttggttatac taattcctat
     2701 agctagttct tgagactcta cttttctctc tagctccttt gacaaagtga tttgagattt
     2761 atttggttcc gttggagtca attctataga ttcttcataa aaatctctat acataaagct
     2821 atcattaatt ttataaaaaa aattaaatgt ttttagcact gccatataac attcaaaaca
     2881 ttcttctata gttggtttac ttccttgagg agaatgaacc aatttattac gtttttctat
     2941 gaaattttta atttcctttc ttatttcttt cggaaactca atattaaagt accctttaaa
     3001 catgctccct aataatttgt ctacaggggg agattgtaaa ttctcaaata ttagagttaa
     3061 atcaggtttc tcttgttgat aaaattcctt tacccctatt tcacaggctg taattatcga
     3121 aataaaccta tctctagtat ttgatatatt tttacttctt ttaaggagta taagagaaaa
     3181 ggggatactt tcgtgtccat gcatgtaatt aactaattgt tgagcttcag aagttatatc
     3241 tctaattata tagttaacgg aattaggaag tgtctttaac tctaatgaaa tttctatcca
     3301 attactatct tctaatctgt atatgctcga agtataattt gattcgtcag caagcatcgc
     3361 tacatcccaa acactgatca taaaatcttt taaacgttca atcatttcag caatattgct
     3421 aaaatcagct aatttttctt tagaataaaa aaaacattta tatcagaatc aataaattct
     3481 atttttttta ttactatatc gtcatcatta tcttgaccat ttatgtttat tgttccctga
     3541 gtggggagta cttctttcaa attcaagtta aatttatatt ccattacttc tcctccaata
     3601 tataaaaatt acttaactgt atccaaattt aatatgtaaa taaatcatta atttttagct
     3661 aaattctatg agaagtttat ttttgttgtt tttgctaatt aactaaaact agtacaaaaa
     3721 atttaaaata gattattaaa cctattgctt attctattca gtcatatata taagcgtcat
     3781 cctttagtaa ctaacatccc acactacaat agtattataa catatctatt caacttcaaa
     3841 ttgttgaaat ttaagccatt tatatttact tttcctcaac tttagaaatg attttactaa
     3901 ccttatatcc gtaatacgcg taacaaacac attcattaat aacaatcaac actaagctat
     3961 attagctttt taaatgacta gggtaaataa aataaggatc aaaaactttt aagtttctga
     4021 ccctttcctt acttccgatt ggttaatagt gatcttttgc agaataaata aatatcgttt
     4081 cattttcaac tctatatatc agtctatgtt catctgtaat tcttctggac cattttccag
     4141 ataaatcatg ctttaatggc tcaggttttc ctaatccagc aaagggggaa cgatcgatat
     4201 cttttattaa cttgttaatc ttttttatat tgcttttgtt tccttgctca tgccaataaa
     4261 gataatcatc ccaagcatca tcagaccaag ccttaatcat cagattcaac ctcgattaag
     4321 tcatgtgttt taaatgcacc tttggagaat tgttcatctc ctcgacgaat tttttccatg
     4381 acgtaattat tagaaagtgt tctcaacgtt tcttgcatag aatcataatc tctttttgat
     4441 aatacaacaa ctgtatcttc tacatcttta cttgttacaa taagtgtttc agcatcctca
     4501 ttaacttgtt tcatataact acgtaaattt tggcggaaat ttgaataagc tactgcttcc
     4561 attcctttca ccccttattt atttctataa aacaattgta cattatattg tacaattaag
     4621 caaatgatta atattctaac ttcttat
//
LOCUS       DARBET010000127         4609 bp    DNA     linear   BCT 07-NOV-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4027
            SAMEA114569841-rid20112183.denovo.133, whole genome shotgun
            sequence.
ACCESSION   DARBET010000127 DARBET010000000
VERSION     DARBET010000127.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA114569841
            Sequence Read Archive: ERR12203666
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4609)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4609)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001972618.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001972618.1
            Genome Coverage       :: 132x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/07/2023 13:18:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,041
            CDSs (total)                      :: 2,975
            Genes (coding)                    :: 2,908
            CDSs (with protein)               :: 2,908
            Genes (RNA)                       :: 66
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 67
            CDSs (without protein)            :: 67
            Pseudo Genes (ambiguous residues) :: 0 of 67
            Pseudo Genes (frameshifted)       :: 26 of 67
            Pseudo Genes (incomplete)         :: 28 of 67
            Pseudo Genes (internal stop)      :: 20 of 67
            Pseudo Genes (multiple problems)  :: 6 of 67
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4609
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA114569841-rid20112183.denovo.133"
                     /isolate="VRE4027"
                     /db_xref="taxon:1352"
                     /country="Denmark"
     gene            complement(84..338)
                     /locus_tag="SCJ86_002743"
     CDS             complement(84..338)
                     /locus_tag="SCJ86_002743"
                     /inference="COORDINATES: protein motif:HMM:NF024673.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
                     /protein_id="HEG1170228.1"
                     /translation="MTATEIRNNKLKKKISTIFFTNKQRYGATKIHQVLLKEGISVSL
                     KHVQKLMKQLNLRSIVVKKYRPQRSNKPIMDRLQLTRQKN"
     gene            complement(449..703)
                     /locus_tag="SCJ86_002744"
     CDS             complement(449..703)
                     /locus_tag="SCJ86_002744"
                     /inference="COORDINATES: protein motif:HMM:NF013677.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HEG1170229.1"
                     /translation="MTRYEENFKQMIVELNQTGRSVRGLAKEYGLSEATIYKWKNLYL
                     PDQSTGLTGKEVAELRKENARLNEELEILKKAAAIFSRKT"
     gene            complement(896..1171)
                     /locus_tag="SCJ86_002745"
     CDS             complement(896..1171)
                     /locus_tag="SCJ86_002745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001196543.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system protein PrgO"
                     /protein_id="HEG1170230.1"
                     /translation="MALLPTNKDKKKIGAEDISIKDDINVELGERKYTAKERKPVQVD
                     PPVLKTIRDLSYAKDIPMYEIVKLAMDAYVDTLNDEERIIYNRRSKD"
     gene            complement(1143..2096)
                     /locus_tag="SCJ86_002746"
     CDS             complement(1143..2096)
                     /locus_tag="SCJ86_002746"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623100.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ParA family protein"
                     /protein_id="HEG1170231.1"
                     /translation="MENLRTAFKTSDKLGKVVMLTGRKGGIGKTTDNDLLAIVSSQLF
                     EKDVLLIDYDQQRNTTSNIGSTYQITSFDRSMSAAIKKGDWVSGITQVSPHLYIMAGS
                     PGSEELNEYLSEKYPDRRKRSLAFIKPLEELRKNFDYIFIDCPPSTDNVVRAFLTAAD
                     YIIAMQELKRYAMEGTEDFINKVLVPIVTNFEESHLQIIGILPVLFSVRRSSQHANYQ
                     KTIDKYGESNIFKSIVKGSDRLEMYGENGIQLNDYVDRRWWAIFADIFTELEERIEYY
                     EQHGDIEGFDYTPKYADSMANHILPLGKEIKLNGIITNEQR"
     gene            2708..4201
                     /locus_tag="SCJ86_002747"
     CDS             2708..4201
                     /locus_tag="SCJ86_002747"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000947690.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="primase C-terminal domain-containing protein"
                     /protein_id="HEG1170232.1"
                     /translation="MLSEIYSAILKNGLRTYKYRNSTMKPAGHIDSSRSGAIFAYRSK
                     QLMNVGRGMVITSEEAILENEKKLTHWTPNTYRFGTYADDYRTVVKGHSEKNLSQINT
                     FVVDIDSKENHQGEIILACLDQVGYMPTLILESDHGYQVYFVLKTPGYVTKKSNFKVI
                     DVAKKISKTIRLQLAEKLSGVDLGCNHFGIARFPQENNIVFKEMNYQYSFADWIQWSM
                     KLESDQKTKEDRERKLIVFPEKKEIRQVDEPWFDMLLHKANIVGGEGRLGRNNVIFTL
                     SLAYYSSGYALDTCEYNMFTFNERLQEPLSEAELVKIVRSAYSGNYQAASREFIIELC
                     QQWVSPTLKNNDLFIQRRGWWKFKKPRDQRKYSHKNEWKEDLLAYLSEKSLASEPYLN
                     LSKKELASQLTMPKRTLDKILNELREENKVFYKVTRGRNGGMIIASVKTLFARVIQLS
                     KEKRLDYSTRITEVFGLNESLVEETFRQLSTPLNPLKEVNLFEVDTG"
     gene            4336..>4609
                     /locus_tag="SCJ86_002748"
     CDS             4336..>4609
                     /locus_tag="SCJ86_002748"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000053907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system protein PrgN"
                     /protein_id="HEG1170233.1"
                     /translation="MSLKNYVYSHPVNVFIIGRLGLSVEVFCELYGFKQGTLSSWVTR
                     EKTVASLPIEFLHGLSLASGETMDQVYDCLCVLEQEYIEYKIANELR"
BASE COUNT         1492 a          774 c          802 g         1541 t
ORIGIN      
        1 gcttgaattc ttgtgaaaaa gttcttcgtg ttctcttaga cataaaaatt cctcctggta
       61 tgttttcttc tagtctacac accttaattt ttctgtctag ttaattgtag cctatccatg
      121 atcggtttat tagacctttg aggtctatat tttttaacta caatcgacct taaatttaat
      181 tgtttcatta gcttttggac atgtttaaga gatactgaaa tcccttcttt taataaaact
      241 tgatggatct ttgtggcacc atagcgttgt ttattcgtaa aaaaaattgt tgaaatcttc
      301 tttttcaact tattatttct gatttctgta gctgtcaacg gtttattctt ataaaaataa
      361 tagacacttc tagggatttc taataaccga cacaacaaag aaacagtgta atccttacac
      421 catttttcaa taaattgaac aagcgaattt aggttttccg agagaatatg gctgcggctt
      481 tttttaagat ttcaagttcc tcatttaaac gagcattttc ttttctcagt tcagctactt
      541 cttttccagt cagtcctgtg gactgatcag gtaaatataa attcttccat ttgtaaatcg
      601 ttgcttcaga taagccatat tctttcgcta accctcgaac agaacgtcca gtttgattca
      661 gttcaacaat catttgttta aaattttctt catatcgggt cataaaaatt catcctctcg
      721 tagataagtt taatttattc ttgtctctac gaaaagctgt ctaagatttg atactaacac
      781 caaatgttgt atacccccaa acaatacaca tattttgaag gattaggatt aataaagcta
      841 aaaatatagt aagtatacta aatatattta ctatatctag tatacttact atattttaat
      901 cttttgatcg tctattatat ataatccgtt cctcgtcgtt taaagtatca acataggcgt
      961 ccattgcaag tttgactatt tcatacatag gaatatcttt tgcataacta agatcccgaa
     1021 tggtttttag tacgggagga tctacttgaa ctggttttct ttctttcgca gtgtactttc
     1081 gttcccctaa ctcgacatta atatcatctt taattgaaat atcttcagca ccaattttct
     1141 tcttatcttt gttcgttggt aataatgcca tttagtttaa tctcctttcc aagaggtagg
     1201 atatgattgg ccattgagtc agcatattta ggtgtataat caaatccttc aatatcacca
     1261 tgttgttcat agtattcaat tctttcttct aattctgtaa agatgtctgc aaaaatagcc
     1321 caccatcttc gatctacata atcatttaat tggataccat tctcaccata catttctaac
     1381 cgatccgagc ctttaacaat gcttttaaaa atgtttgatt caccatactt atcaattgtt
     1441 ttttgatagt ttgcgtgttg agaggaacgt cgcactgaga aaagaacagg caaaattcca
     1501 atgatttgaa gatgagactc ttcaaaattc gtgacaatcg gaactaaaac tttattaata
     1561 aaatcttctg ttccttccat tgcgtaccgt tttaattctt gcattgcaat aatataatct
     1621 gcagcagtta agaatgcacg aaccacatta tctgttgatg gtgggcagtc gataaagata
     1681 taatcgaaat tttttctcaa ttcttctaaa ggtttaatga aggccaatga tctcttacga
     1741 cgatcaggat atttttcgga caagtattca ttaagttctt cagatccagg agagccagcc
     1801 ataatatata aatgtggact tacttgagta atacctgaaa cccagtcccc ttttttgatt
     1861 gctgcagaca tggatcgatc aaagctagta atttgatacg ttgatccaat gttggaagta
     1921 gtatttcttt gttggtcata atcaattagc aacacgtctt tttcaaaaag ttgactagaa
     1981 acaatcgcta ataaatcatt gtctgtagtc ttaccaatgc cgccttttct acctgtaagc
     2041 attacaactt ttcctagttt atctgatgtt ttaaatgcag tccgtaagtt ttccatagtt
     2101 ccctccaatt tcctatacta aatatattta gtatacttca tatacttaat atattcgata
     2161 tatctaatat actccaaatt gataagaatt cagactttat aaggaatata gtggatatac
     2221 taaatatatt tgctatattc agtatatcag atatattcaa aatgtcaaaa tatatttgtg
     2281 cgttttgcgt tgacaattta gagtgaaaag aggtaaactt aattctatag aaaccacaga
     2341 taattcacta tttacaaagt agcaaaagca gtggtactct ttaaacagaa aaattacata
     2401 aaaaaaagag acggaaataa ttgattttag cagaatcaat tatttctgaa tcacatggtt
     2461 aggacatgtg ttcacgctcg tctcaagaat atgtaagtag ctaccaacta cttatattaa
     2521 aagttgatcg gatacctttt ccgattgcct ttattctacc aaaaaaaggc gagcaactca
     2581 acagaagaac gtttatttag tgtacttaaa ttaaatattc ttaaacactg aagagagaac
     2641 acatactatg gctgagatta aagctaaact gtgttctctt tttgtttgag aaaggacgtt
     2701 acaatcgatg ttgagcgaaa tttattcagc tattcttaaa aacggcttac gtacatacaa
     2761 atatcgaaac agcaccatga aacctgcagg tcatattgat tccagtagga gtggtgctat
     2821 ctttgcgtac cgtagcaagc aattaatgaa tgttgggcgt ggtatggtca ttacttcgga
     2881 agaagctatt ttagagaacg aaaagaaact aacgcattgg acacctaata cctatcgctt
     2941 tggtacttat gcggatgatt atcgaacagt ggttaaaggt cattctgaga aaaacttaag
     3001 tcaaatcaac acatttgtag ttgatatcga ttcaaaagag aaccatcaag gcgaaattat
     3061 tttagcctgt ttagatcaag tgggctacat gccaacgcta attttagaat cagatcatgg
     3121 ttatcaagtt tattttgtac taaagacacc aggctatgtc acaaagaaaa gcaatttcaa
     3181 ggtgatcgat gtagcaaaaa aaatttcaaa aacaattcgt ctacagttgg ctgaaaaatt
     3241 gtccggagtg gatctaggat gtaaccactt cggaattgct cgattcccac aagaaaataa
     3301 tattgttttt aaagaaatga actatcagta ttcttttgct gactggattc aatggtcgat
     3361 gaagcttgaa tcagatcaaa aaacaaaaga agatcgagaa cgaaaactaa ttgtctttcc
     3421 tgagaaaaaa gaaattcgtc aagtagatga accgtggttc gatatgttac ttcataaagc
     3481 aaacatcgtc ggcggagaag gacgtcttgg tcgaaataat gtgattttca cgctttcttt
     3541 agcttattac tcttcaggtt atgcattgga tacttgcgaa tacaatatgt tcacattcaa
     3601 cgaacggtta caggaaccgc tatctgaagc agaattagta aagattgtac gtagtgctta
     3661 ttctggtaat tatcaagcag caagcagaga attcattatt gaattgtgcc agcaatgggt
     3721 ttcaccaacc ttaaaaaata atgatctgtt cattcaacga cgtggctggt ggaaatttaa
     3781 aaagccacga gatcaaagaa aatatagtca taaaaatgaa tggaaagaag atctattggc
     3841 ttatctaagc gaaaaaagtc ttgctagtga gccttatttg aacctgtcaa aaaaagaatt
     3901 ggcttcacaa ctaacgatgc ccaagcgaac acttgataag atcttaaatg agttacgaga
     3961 ggagaataaa gtcttctaca aggtaacacg aggaagaaat ggcggaatga tcattgcttc
     4021 agtcaaaacg ttgttcgcga gggttattca actaagtaaa gaaaagagac tagattattc
     4081 aacaaggatc acagaggtat ttggactcaa cgagagctta gtagaagaga catttagaca
     4141 attatcaacc cccttgaacc ctcttaaaga agtgaatctt tttgaagtag atacaggttg
     4201 aaaaataaaa ggtgcaaatt gccttataca atatatctat gatacttctg taggtgttgc
     4261 tccctttgcc tagctacgtg taggtggtat acttataact gcgtatgcag tcttatgtat
     4321 catggaggaa gttgtatgtc gttaaaaaat tatgtctatt cacatcctgt taatgtgttt
     4381 atcattggtc gtttgggatt atctgtggaa gtgttctgtg agctttatgg ttttaagcag
     4441 gggactttgt cttcttgggt taccagggaa aaaacagttg cttctttgcc gattgagttt
     4501 cttcatgggt tgtccttagc ttctggggag actatggatc aggtatatga ttgtttgtgt
     4561 gtgttagagc aggagtatat tgagtataaa atagctaacg agttaagaa
//
LOCUS       DARBET010000128         4473 bp    DNA     linear   BCT 07-NOV-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4027
            SAMEA114569841-rid20112183.denovo.134, whole genome shotgun
            sequence.
ACCESSION   DARBET010000128 DARBET010000000
VERSION     DARBET010000128.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA114569841
            Sequence Read Archive: ERR12203666
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4473)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4473)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001972618.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001972618.1
            Genome Coverage       :: 132x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/07/2023 13:18:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,041
            CDSs (total)                      :: 2,975
            Genes (coding)                    :: 2,908
            CDSs (with protein)               :: 2,908
            Genes (RNA)                       :: 66
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 67
            CDSs (without protein)            :: 67
            Pseudo Genes (ambiguous residues) :: 0 of 67
            Pseudo Genes (frameshifted)       :: 26 of 67
            Pseudo Genes (incomplete)         :: 28 of 67
            Pseudo Genes (internal stop)      :: 20 of 67
            Pseudo Genes (multiple problems)  :: 6 of 67
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4473
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA114569841-rid20112183.denovo.134"
                     /isolate="VRE4027"
                     /db_xref="taxon:1352"
                     /country="Denmark"
     gene            321..1052
                     /locus_tag="SCJ86_002749"
     CDS             321..1052
                     /locus_tag="SCJ86_002749"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322951.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="WxL domain-containing protein"
                     /protein_id="HEG1170234.1"
                     /translation="MMKKEIVLTAAMMLFSMVASTTFVSATEVYPKEYNTEGTITFEA
                     GDEGVTPPVDPENPDPNKPVDPSDPPSPGTGGALSIDYGSKFKFGTQKISTADKTYYA
                     AADVMNDGSRKPTYVQVTDRRSTLSGWKLSVSQPEQFKTASGDELVGAQLKFTKGQAV
                     SLVDPTYTPQTVNSELTLTPGGNNTLAINAKSGTGVGTWVYRFGANENENQDAVQLSV
                     PGKSVKLAQQYSTKLVWTLEDTPNN"
     gene            1197..3224
                     /locus_tag="SCJ86_002750"
     CDS             1197..3224
                     /locus_tag="SCJ86_002750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304041.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MucBP domain-containing protein"
                     /protein_id="HEG1170235.1"
                     /translation="MKKRRIVLILSSLLVTNIFPIIGYSLELNQLEHGNRRDEESKLT
                     STFDSLIESNQTQESSEDLSISSSGETTDNVEAEGSDKQGLEDAPISAVELPDDPNII
                     SIDKIFQTPIGTSTSILENGKLLQLNPAERSQRGAIWSNKKISLLSDFTFKSYLYLGN
                     QRDRAGDGMTFTLTGDPRMASNPSQVIGSPGMGIGSYSTRSGQPYIANALSIEFDTYK
                     NSGSSDRMDREITADNRGHGHVAFVTPKPNNNNYSGEHTGVTVAPTFLSNGTWRLLII
                     KWDAEQRSLTYDLEGVGHSSRIIPDLIRQFGSTEVNWGFTSSTGTFFQENAVAVTRLP
                     SNVTSTADVKVNDSDYGAIAEAGGNDKITIRNTLKVEGDFLEYKKMQTSIDLPQELTI
                     PEHILINGNQIDAKDILITGNRVTLNLTDYFIAGDTLIIELETTLQEVIPEKVLTMHF
                     EYLENGNLIEQSNEINLKIAKLKEIDIHVYYKDKDSQIDIAEMKTITGKIGEKYKETP
                     IDIEGYVFDSDSANTEGIFSENTEDIYFYYRKGKLYLSTAPQYVNFGKHKISSRPLSE
                     FGQFSGGLKIIDERAFGSWKLQLKQEKVLTNGDFELPEALSFVTSENTTVIGTSAVTI
                     FESDQKGESDLSSLLDSTAHRGIRIDIPVENQRIGTFEGKLSWILADVPGN"
     gene            3332..4429
                     /locus_tag="SCJ86_002751"
     CDS             3332..4429
                     /locus_tag="SCJ86_002751"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307341.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF916 and DUF3324 domain-containing protein"
                     /protein_id="HEG1170236.1"
                     /translation="MIKGAIFLRPKQQLFITLLVILGALFNNISVSASAFNFSVTPIT
                     SENQIDKRKTYFDLQLDPDQEVEVKAELRNDTEKEVKIDISVNSATTNSNVMVEYGKN
                     EIEKDMSLIFDLVDYVSYPQIVTLKPKSVQTVAFHVRMPNERFDGVLAGGITFQEIQT
                     EKDQTETKDQSLSIENEYTYIIALLMQQTLNEVAPNLTLHEVKPDQINARNVILANVQ
                     NDQKTYINQVVIETKITKKGHSEVLYQEEKEGLQIAPNTNFSFPTALNGQPLTPGEYH
                     LTMTVLGNENENGKFSRKKGNTTINYTNQWVFEKDFTIDGKVAKELNTTDVTIKKDNN
                     WIYILVASLFLLFILLCILLFVLRKKEEKEK"
BASE COUNT         1572 a          717 c          923 g         1261 t
ORIGIN      
        1 aaaatataat atacattttt tatttaatta taattaatat tgattgaagt ctaggcagtc
       61 aagtcacatt ttgatagata ggagggggac ttacgaatta ttttaaaggc tatgacagct
      121 gtatttctag attgtgtttg catcaagttg caataaaaac tagaaatcaa atcgggctga
      181 taatcattta taactaatcg atacatagga ccaaatatag gtctatgttc ttttatttca
      241 taagtatcgt aaaacagaat tttgctatgg attaatccaa tcgactgata tgtttagtaa
      301 ataaagaaag aggaatgtca atgatgaaaa aagaaatcgt attaacagca gcaatgatgt
      361 tattcagcat ggtagcaagt acgacttttg tttctgcaac tgaagtatac ccaaaggaat
      421 ataacacaga aggaacgatt acatttgaag caggagatga gggtgtaact cctcctgttg
      481 atccagagaa tcctgatccc aataaacctg tcgatccatc agatcctcca agccctggaa
      541 caggaggggc attatcgatt gattatggat caaaattcaa atttgggact caaaaaattt
      601 ctactgcgga taaaacgtat tatgcagctg ctgatgtgat gaacgatggg tctagaaaac
      661 cgacatatgt ccaagtgaca gataggagaa gtaccttatc tgggtggaaa ttaagtgtct
      721 ctcaaccaga acagtttaaa actgccagtg gggatgaact agttggagca cagctgaaat
      781 ttacgaaagg gcaggctgtt tctttggttg atccaacata tacgccgcaa acggtgaatt
      841 ctgaattaac gttgacaccg ggaggaaaca atacgttagc aattaatgct aaatcaggaa
      901 caggtgtggg gacttgggtc tatcgatttg gtgcaaatga aaatgaaaat caagatgcgg
      961 tacagctttc ggtacccggt aaatctgtca agttagcgca acaatattcc actaaattag
     1021 tttggacatt agaagataca cctaacaact agtttactga aaagaactgt taaaaataaa
     1081 tacgtagtca tattgtcttg cgaggtaatc gagcaaaaat tgtcaaaggc taggctaaat
     1141 ttgtttggat acgaatagtt catctaggaa tttaacgatc aggaggaaaa gatatattga
     1201 aaaagaggag gatcgtgctc attttaagca gtctcttagt gactaatatc tttccgatca
     1261 tagggtactc tttggaattg aatcagttgg agcatggaaa tagaagagac gaagaaagca
     1321 aactcacttc gacattcgac agtttaatag aatccaacca gacacaagaa agcagtgagg
     1381 atttaagtat ttcgagtagt ggagagacca ctgacaacgt agaagctgag ggttcggaca
     1441 aacaaggact agaagatgca ccaatttctg cggttgagct gcctgacgat cccaatatta
     1501 tttctatcga taaaattttt caaacaccta tcggtactag cacgtccata ttggaaaatg
     1561 ggaagttact gcagttgaac ccagcagaaa ggtctcaaag aggagctatt tggtcaaata
     1621 agaagattag tttactctcg gattttacat ttaaatctta tttatactta ggaaatcaac
     1681 gagatagagc gggagatggc atgaccttta cattgactgg cgatcctaga atggctagta
     1741 atcccagtca agttattgga tcgccaggta tgggaattgg ctcatattct actcgttctg
     1801 gccaacctta cattgcgaat gcgctctcga ttgagtttga tacgtataaa aatagtggtt
     1861 cttctgatcg aatggatcga gagataactg ctgataatag agggcatgga catgtcgctt
     1921 ttgtcacgcc taaaccaaat aataataatt attctggaga gcatactggc gtgacggtag
     1981 ctcctacatt tctttctaat ggcacatggc gcttgttaat aattaaatgg gacgcggagc
     2041 aaagaagtct tacttatgat ttggaaggtg tcggtcactc ttctcggatt atacctgatc
     2101 tcattaggca gtttggttct acagaagtaa attgggggtt tacttcttca acaggtactt
     2161 tttttcaaga aaatgctgtc gctgtcacgc gacttccatc gaatgtcaca tcaacagctg
     2221 atgtaaaagt caacgatagt gattatgggg ccatcgctga agctggagga aacgataaga
     2281 taactatcag aaatacattg aaggtcgagg gggattttct tgaatataag aaaatgcaga
     2341 ctagtatcga cctcccacaa gaattaacta ttcccgagca tatcttgatc aatggaaatc
     2401 aaattgatgc caaagatatc ttgataaccg gaaatcgagt cacgctaaat ctgacagatt
     2461 attttattgc tggagatacg ttaataatcg aattggagac aactttgcag gaagtaattc
     2521 ctgaaaaagt actcactatg cattttgaat accttgaaaa cgggaatttg attgagcaat
     2581 cgaatgaaat aaacctaaag atcgcaaagc tgaaggagat agatatccat gtttattata
     2641 aagataaaga cagtcagatt gatattgctg aaatgaaaac aatcactggg aaaataggag
     2701 agaagtataa agaaactcct attgatattg aagggtatgt ttttgactcg gattcagcga
     2761 atacagaagg catcttctcc gaaaatacag aggatatcta tttttattac agaaaaggca
     2821 aactttatct ctcaactgct cctcaatatg tcaattttgg aaaacataaa atatccagta
     2881 gaccattaag tgaattcgga cagttttcag gtgggttgaa aattatagat gaacgggctt
     2941 ttggaagttg gaaattacaa ttgaaacaag agaaagttct tacaaatgga gattttgagc
     3001 ttccagaagc tctttccttt gttacctctg aaaatacaac ggttataggg acatccgctg
     3061 ttacgatttt cgaaagtgat caaaagggtg aaagcgattt gtcctctctt ttagattcta
     3121 cagctcatcg tggaatcaga atagatattc ctgtagaaaa ccaaagaatc ggaacctttg
     3181 aggggaaact atcttggata cttgcagatg tcccaggaaa ttaacaggaa gagaagaata
     3241 taacaaaatt attgagtttc aagaagggca caggtaccat aggctcttgt gtcccacaga
     3301 gacaagattt tgttatagtc ttctcttaca tatgattaaa ggagcgattt tcttgagacc
     3361 aaaacaacaa ttatttataa cactcctagt tattttaggg gcacttttta acaatatatc
     3421 agtgtcagct agtgcattta atttttcagt gacgccgata acttcagaga atcaaatcga
     3481 caagagaaaa acttacttcg atcttcaact tgatccggat caggaagtag aagtaaaagc
     3541 ggaattgaga aatgatacag aaaaagaagt gaaaatcgat atttctgtga atagcgcaac
     3601 caccaattca aatgtgatgg ttgaatacgg aaaaaacgag attgaaaaag acatgagcct
     3661 gatctttgat ctggtagatt atgtgagcta tccacagata gtgacgctaa aacctaaaag
     3721 tgtccaaacc gttgcttttc atgtcaggat gccgaatgag cgatttgatg gtgtattggc
     3781 tggtgggatc acattccaag aaatccagac ggaaaaggac caaacagaaa ctaaggatca
     3841 aagcttatcg attgagaatg aatacaccta tattattgct ttgttgatgc aacagacact
     3901 gaacgaagtt gctccgaacc ttactcttca tgaagttaaa ccagatcaaa tcaatgcaag
     3961 aaatgtcatc ttagcaaacg ttcaaaatga tcaaaaaacc tatatcaatc aagtagttat
     4021 tgaaacaaaa attacaaaaa aaggacattc tgaagtttta tatcaagagg aaaaagaagg
     4081 attgcagatt gcgccgaata caaatttttc ttttccaact gctttaaatg gtcaaccgct
     4141 aaccccaggt gaatatcacc ttacaatgac tgttttagga aatgaaaatg aaaatggaaa
     4201 attttcgaga aaaaaaggaa atactacgat caactatacc aatcaatggg tatttgaaaa
     4261 agattttaca atagacggaa aagtagccaa ggaattgaac acgacggatg ttacgattaa
     4321 aaaagacaat aattggattt atatcttagt agcttcactt ttcttattgt ttattttatt
     4381 atgcatactt ttgtttgttt tgagaaaaaa agaagagaaa gaaaagtaag gttaagataa
     4441 caaatagtaa atttttatta ttgtaggatg gtt
//
LOCUS       DARBET010000129         4345 bp    DNA     linear   BCT 07-NOV-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4027
            SAMEA114569841-rid20112183.denovo.135, whole genome shotgun
            sequence.
ACCESSION   DARBET010000129 DARBET010000000
VERSION     DARBET010000129.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA114569841
            Sequence Read Archive: ERR12203666
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4345)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4345)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001972618.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001972618.1
            Genome Coverage       :: 132x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/07/2023 13:18:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,041
            CDSs (total)                      :: 2,975
            Genes (coding)                    :: 2,908
            CDSs (with protein)               :: 2,908
            Genes (RNA)                       :: 66
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 67
            CDSs (without protein)            :: 67
            Pseudo Genes (ambiguous residues) :: 0 of 67
            Pseudo Genes (frameshifted)       :: 26 of 67
            Pseudo Genes (incomplete)         :: 28 of 67
            Pseudo Genes (internal stop)      :: 20 of 67
            Pseudo Genes (multiple problems)  :: 6 of 67
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4345
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA114569841-rid20112183.denovo.135"
                     /isolate="VRE4027"
                     /db_xref="taxon:1352"
                     /country="Denmark"
     gene            complement(387..1853)
                     /locus_tag="SCJ86_002752"
     CDS             complement(387..1853)
                     /locus_tag="SCJ86_002752"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628959.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HEG1170237.1"
                     /translation="MNNKQLRWFTVALIAFNMVWGMGNVVNNYAQQGVSVVTSWLLIL
                     AIYFIPYALIVGQLGSAFKDSKGGVSSWVENTTSNKRLAYYAAWTYWVVHIPYLAQKP
                     QAILIAGGWAVQGNGNIVNTMSVQMVAVISLIIFLAFLYLSTKGLSTLKVIGGLAGTA
                     MFVMSILFILLAVTAPFINPTMEFATPHMNQVKTYIPTFDFSYFTTVSMLVFAVGGAE
                     KISPYVNSTRNPAKEFPKGMIFLAAMVGICAVLGSVAMGMLFASDNIPKDLMANGAYS
                     AFQILGNYYGIGNSLMIIYALTNMIGQISALAFSIDAPLKILLADADPEFVPNWLRKR
                     NRKGTLVNGYLLTGILVGIIILLPIFGIKDMNELVKWLTNLNSVVMPMRYLWVFLAYM
                     MLNKAYKKFNSEYKFVKNPKIGFIFGAWCFAFTAFACILGMVPKVDYTADPKAWWFQL
                     FTNIITPIVLILLGMILPAIARRDKNKKITEPETVVQP"
     gene            complement(2144..4228)
                     /gene="cadA"
                     /locus_tag="SCJ86_002753"
     CDS             complement(2144..4228)
                     /gene="cadA"
                     /locus_tag="SCJ86_002753"
                     /EC_number="7.2.2.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296577.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cadmium-translocating P-type ATPase"
                     /protein_id="HEG1170238.1"
                     /translation="MKSYRLEGLDCANCAMKIEKGVQKINGVKEATVNFTSGKLTIDA
                     EEDHLATIEQETKKVVKELEPDVKVTEIDKEKVSEHGNEKERNTLFRILFSLAGIALL
                     LLFDFNEPIRLIGYLLIYLLIGYDVVKKAVMNIVKGKIFDENFLMSVATIGAMLIGEY
                     PEAVAVMLFYQIGEYFQGLAVSHSRKSIRELMAIRPETAHVQTTEGLMTVNPEDVQIG
                     QFVLVKPGERVPLDGTIIEGESLVDTSALTGESVPKSVYVGETVLSGFINKNKPFLVQ
                     VEKSYENSTISKLLELVENASSKKAPAENFITKFARYYTPVVVGLAVLLAVLPPLVVS
                     GAAFSEWIYRALTFLVISCPCALVISVPLSFFGGIGGASKIGVLVKGSNYLELLAQTE
                     TVVFDKTGTLTKGDFSIQTIDTKIDPKKFMQYIASAEQFSTHPIAQSVLEGYAGPLLP
                     TTNIQEFAGEGILAEVDGKQVLAGNHKLMERFEISFPLSQEIGTLLYLAIDQSYSGYL
                     VIADTLKEDAVDALVQLKQAGVKNTVMLTGDSKKIADHIGKQVGVDKIYSELLPEDKV
                     QRLEEILQRNNKKTAFVGDGINDAPVLARADVGIAMGGLGSDAAIEAADVVIMNDQPS
                     KIAEAIHLAKKTLKIVKQNIVFAIGIKILVLALGAFGFASMGDAVFADVGVTVLAVLN
                     AMRSLHVKKEDE"
BASE COUNT         1309 a          927 c          697 g         1412 t
ORIGIN      
        1 aataaatggt gttcaatcag ctgaccttca gcattagctc aaaaagcggc aaaatatgag
       61 gagctatttt tccgtttgtt tctctaatac tcaggtcaaa tcgctgattt tacaacctct
      121 tccttaaacg gtgttccaaa gcttgcatct gcggtaataa gcccagccaa acgaaaaata
      181 tgaggggcta tttttgtttg gctgttctta ttacttgatg caggacagct ttttcacaac
      241 ctctgattca cggtgttcca gaagtagctt cttcgaaaaa caagccgaaa cttcacaaaa
      301 atttgagaag caattttcgt aaattccttc ttatttcttg aagctgacca cttctgtcac
      361 aacctcttgt aacaagtatt tctattttaa ggctgaacca ctgtttctgg ttcagtgatt
      421 tttttgtttt tgtctcgacg agcaattgct ggcaagatca tacctaataa gatcaagacg
      481 attggtgtga tgatattcgt aaataattgg aaccaccatg ctttaggatc agcagtataa
      541 tctacttttg gcaccattcc taggatacat gcaaacgcag tgaaggcaaa gcaccaagca
      601 ccaaagataa aaccgatttt tggattttta acaaatttat attcagaatt gaatttctta
      661 taagctttat tcaacatcat atatgccaag aacacccata ggtaacgcat tggcatgaca
      721 actgagttca agttcgtcaa ccattttact aattcattca tatctttgat accaaaaatc
      781 ggcaacaaaa taatgattcc tactagtata cctgtcagaa gataaccatt caccaatgta
      841 cctttacgat tacgtttacg tagccagttt ggtacaaatt ctggatccgc atctgctaac
      901 aagattttta gtggtgcatc aattgaaaag gctaatgcgg agatttgtcc aatcatgttt
      961 gtcaatgcgt aaatgatcat caatgagtta ccgataccat agtaattacc taaaatttga
     1021 aaagcactgt atgccccatt tgccatcaag tctttaggaa tattatcact agcaaacagc
     1081 atacccattg cgactgagcc taatacagca caaatcccta ccattgctgc taagaagatc
     1141 atccctttag gaaattcctt tgcaggattt cttgtagagt tgacataagg ggatattttt
     1201 tccgcccctc caactgcaaa tactagcatc gaaacggttg tgaaataact gaaatcaaat
     1261 gttggaatat atgttttcac ttgattcata tgtggtgtgg caaattccat cgttggattg
     1321 atgaacggtg ccgttacagc taaaagaata aataagatcg acatgacgaa catcgctgtt
     1381 ccggccagtc cgccaatcac tttcaaagta gaaagtcctt ttgttgataa atataaaaag
     1441 gcaagaaata taataagtga aataactgct accatttgta ctgacatcgt atttacgata
     1501 tttccatttc cttggactgc ccaaccacca gcaatcagaa ttgcttgagg cttttgtgcc
     1561 aaataaggaa tatggacgac ccagtaagtc caagcggcat agtatgccag tcgtttatta
     1621 gatgtcgtat tttcgaccca tgaactgacg cctcctttac tatccttgaa ggctgatcct
     1681 aattgtccaa cgatcaatgc gtatggaata aaataaatgg ccaaaatcaa tagccaagag
     1741 gtgacaactg atactccttg ctgagcatag ttattaacta cattccccat gccccaaacc
     1801 atgttaaatg caattaacgc aacagtaaac cagcgtaatt gtttgttgtt cattcgttct
     1861 ctcctttatg ataaaaattt gttatccaga tgtaatcata ttcaattttt aacaaactta
     1921 caagacagtg ttattttatg tgtgtattct tttatttaat acacatcgtt ttttcatatt
     1981 ttttccatca aaaaaataag ttattttctt ttatttcatt gaccttattg aatttcaagc
     2041 tattttgaat tttctgcaaa attaaaaaca ttagttattt tataatccta tctattttct
     2101 ttacattttt cattaagaaa aggatataaa aaaaagagga cgttcattcg tcctcttttt
     2161 ttacatgcaa gcttcgcatg gcattcaaaa cagctaatac tgtcacccca acatctgcaa
     2221 atactgcatc tcccattgat gcaaaaccaa aagcacctaa ggcaagtacc aaaattttta
     2281 tcccaatagc aaagacaatg ttttgtttga cgattttcaa tgttttcttt gctaaatgga
     2341 ttgcttctgc aatcttcgat ggttgatcat tcatgatgac tacatctgct gcttcaattg
     2401 cagcatcgct tcccaatcca cccattgcga ttcctacatc tgctcttgct agcaccggtg
     2461 catcgttgat tccatctccg acaaatgcgg tctttttatt gtttctttgt aagatttcct
     2521 ctaaccgctg cactttatct tcaggaagca attcgctata tatcttgtcc acgcctactt
     2581 gcttcccgat atgatcagca atttttttcg agtcgcccgt caacataact gtgttcttca
     2641 caccagcctg ttttaattga accaatgcgt ctacggcatc ttctttcaag gtatctgcaa
     2701 taacaagata accactgtaa ctttgatcga tcgctagata aagcaatgta ccgatttctt
     2761 gacttaaagg aaaagagatt tcaaatcttt ccatcaattt gtgattacct gctaatactt
     2821 gtttgccatc aacttcagct aagatccctt cacctgcaaa ttcctggata ttagttgttg
     2881 gtaaaagcgg acctgcgtag ccttctaaaa cagattgagc aatcggatgt gtagaaaact
     2941 gttctgcact cgcaatatat tgcatgaatt tctttggatc gatttttgta tcgatcgtct
     3001 ggatcgaaaa gtcacctttc gttaatgtgc cggttttatc gaataccact gtctcggttt
     3061 gcgcaagcaa ctccaagtaa tttgaacctt ttactaatac accgatttta cttgcgcctc
     3121 cgataccgcc aaagaaacta agtggcacgg aaatcactaa tgcacatgga catgagatca
     3181 ctaaaaatgt caatgctcga tagatccatt cagaaaacgc cgctccagaa acaacaagtg
     3241 gaggtaaaac agctaataaa acggccaatc ctacaacgac tggtgtgtaa tagcgcgcga
     3301 atttagtaat gaaattttca gctggtgctt ttttactgct ggcattttct actaattcta
     3361 ataatttgct gatagtagaa ttttcataag atttttctac ttgaactaaa aatggtttat
     3421 ttttattgat aaatccgctt aatactgttt caccaacata tacagattta gggactgatt
     3481 ctcctgtaag tgctgacgta tcgactaatg attctccttc gatgatcgtt ccatctaacg
     3541 gtacacgttc accaggcttc acaagtacaa actgaccgat ctgcacgtct tcagggttca
     3601 cggtcatcaa tccttccgtc gtctgcacgt gagctgtttc tggacggatc gccatcaatt
     3661 cgcgaatcga ttttcttgaa tgactgactg ccaatccttg gaaatactcg ccgatttgat
     3721 aaaatagcat cactgctacg gcttctggat attcaccgat caacatagcg ccaatcgtag
     3781 caacagacat aagaaaattc tcatcaaaga tttttccttt gacgatattc atcactgcct
     3841 tcttcactac atcatatcct atcagcagat aaatcaataa ataccctatc aagcgaattg
     3901 gttcattaaa atcaaataat agcaataaag cgatacctgc aagggaaaat aaaatccgga
     3961 acagcgtatt tctctctttt tcatttccat gttcagatac tttttcctta tctatttctg
     4021 ttactttgac atctggttct aattccttca ctactttttt tgtttcttgt tcaatcgtag
     4081 ccaaatgatc ttcctctgcg tcaatcgtta gttttccaga agtgaagttt accgttgctt
     4141 ccttgactcc attgatcttc tgaactcctt tttcgatttt cattgcacag ttcgcacaat
     4201 ctaaaccttc taatctgtaa cttttcattt taatgacctc ctcgcctcaa catatgaaca
     4261 tctcttcata actaattata aatgaatggt tattcatatg tcaaggtatc cgaaagaaaa
     4321 aatgaaaaac atctgcatcg gaaaa
//
LOCUS       DARBET010000130         4310 bp    DNA     linear   BCT 07-NOV-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4027
            SAMEA114569841-rid20112183.denovo.136, whole genome shotgun
            sequence.
ACCESSION   DARBET010000130 DARBET010000000
VERSION     DARBET010000130.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA114569841
            Sequence Read Archive: ERR12203666
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4310)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4310)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001972618.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001972618.1
            Genome Coverage       :: 132x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/07/2023 13:18:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,041
            CDSs (total)                      :: 2,975
            Genes (coding)                    :: 2,908
            CDSs (with protein)               :: 2,908
            Genes (RNA)                       :: 66
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 67
            CDSs (without protein)            :: 67
            Pseudo Genes (ambiguous residues) :: 0 of 67
            Pseudo Genes (frameshifted)       :: 26 of 67
            Pseudo Genes (incomplete)         :: 28 of 67
            Pseudo Genes (internal stop)      :: 20 of 67
            Pseudo Genes (multiple problems)  :: 6 of 67
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4310
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA114569841-rid20112183.denovo.136"
                     /isolate="VRE4027"
                     /db_xref="taxon:1352"
                     /country="Denmark"
     gene            complement(65..1285)
                     /locus_tag="SCJ86_002754"
     CDS             complement(65..1285)
                     /locus_tag="SCJ86_002754"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015509263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain-containing protein"
                     /protein_id="HEG1170239.1"
                     /translation="MKLIGIVGSAAELSYNRKLLTFIQKQYHELFNLEIIEIKDLPLF
                     NQDDDQTNSPAIQQLNRKILMADGVIIATPEHNHTVPAGLKSALEWLSFKIHPLENKP
                     VMVVGASYYDQGTSRAQLHLRQILDAPGVNALVMPGNEFLLGKAKEAFDQEDRLIDPR
                     TVDFLGSTLASFVKFIKVVSTLAGPKKLPPEDLHATGTIATTIPDVDMSDDHWLEKAS
                     EAVGAVSGNTYVKLNRGLLTVDQINYFLDSMPMELTFADNNNQFLYYNLRQEAKEMLA
                     ARVPGQVGNPLANCHPEKSYKNVEWVIQQLRSGATDAVRVHVPKQVPEKYIVHNYQAM
                     HDENGEYAGINEYILDFKPIIEWYLQQTGQQLVGNEDAVSSASVHGGKTDAVTSASTH
                     DETAVDAVSSASVK"
     gene            complement(1305..1916)
                     /locus_tag="SCJ86_002755"
     CDS             complement(1305..1916)
                     /locus_tag="SCJ86_002755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent oxidoreductase"
                     /protein_id="HEG1170240.1"
                     /translation="MKMKFIGLVGTNSTRSTNRELLQFMKNYFVNEAEIEIVEIKELP
                     LFNKPENTELPNVVKEIAGKIEQADGVIISTPEYDHAVPASLINALNWLSYGIFPFVD
                     KPVMITGASYGTLGSSRAQAHLRQILDSPELKARIMPSSEFLLGHSLQAFDEQGRLID
                     EEQVAKLRELFKDFLLFVTITSQLQHAHQANKREAESFSWETI"
     gene            complement(1959..2891)
                     /locus_tag="SCJ86_002756"
     CDS             complement(1959..2891)
                     /locus_tag="SCJ86_002756"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350224.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD:protein FMN transferase"
                     /protein_id="HEG1170241.1"
                     /translation="MNQVIEETKIWQMMGTTITLQVGHEEPSRLLAELGEWLHVYEHR
                     FSAHDATSELMAINQAAGRQAVIVHPELFELIKLGKAHSCARNSYLNIAIGPVVQKWH
                     IGFEDALVPTKAEITELLLLTDPHQIQLDEKMKTVFLTKPGMMIDLGALAKGYIADKL
                     ITHCRERNAAFALVNLGGNIVVFGESPRRSDGAWRIGLRNPKGSREESLAVVKLNNQS
                     LVTSGIYERYLQNGSSVYHHIIDPKTGFPVKNDVASLTILSDSSVDGEIWTTRLFGAS
                     SLEVLKALEEQPGIEGAVVTKENQFLYTKGFEKI"
     gene            complement(3312..4172)
                     /locus_tag="SCJ86_002757"
     CDS             complement(3312..4172)
                     /locus_tag="SCJ86_002757"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350223.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HEG1170242.1"
                     /translation="MNIEKFITARKAANLSQKEVSEGICTQATLSKFENNGQVPSLKI
                     LIKLCNRINLPLVELFSSMNVINTQLDEQLEKAEFSLITSEYEQLTQILAAIDPKVID
                     ESELIARYYYLKGFEMIFTNAPITDVLFHFDQLLMAETANTLFRLLAYTGIGMVYARE
                     KELEKAEVYFNRVFEKIYTYPIRTTEDIWRVLNIVFHCGVFFAQDGDLETSDALLNYA
                     IDICSDNHLTYYLARAAAQLAKNAQAKNKSKAEVLELIQDALAFAKINKNKKLIDELN
                     HMKKMILLIN"
BASE COUNT         1230 a          866 c          707 g         1507 t
ORIGIN      
        1 agcttaaatc atagataccg taagggactt tattctttat ttaaaacttt gcaacagaac
       61 ctttttattt tacagaagca ctcgaaactg catctactgc agtttcatcg tgtgtgctgg
      121 cgctggttac tgcatctgtt tttccaccat gaacagatgc acttgaaacc gcatcttcat
      181 ttccaacaag ttgttgaccc gtttgttgta aataccactc aatgattggt ttgaaatcaa
      241 gaatgtattc attgattcca gcatactctc cattttcatc atgcattgct tggtaattat
      301 gcacaatata tttttctggc acttgttttg gtacatgaac acgtaccgca tctgttgctc
      361 cgcttcgcag ttgttgaatt acccactcta catttttata cgatttttct gggtgacagt
      421 tggctaatgg attccctacc tgaccgggca cacgagcagc taacatctct ttcgcttctt
      481 ggcgcaaatt ataatataaa aattggttat tattatctgc aaaagttaat tccatcggca
      541 tcgaatctaa aaagtaattg atttgatcca ctgtcaataa cccacgattt aatttgacgt
      601 aagtatttcc agaaacggca ccgactgctt cagaagcttt ttccaaccaa tgatcatctg
      661 acatatcaac atcgggtatt gtcgtagcaa ttgttcctgt cgcatgcaaa tcttctggtg
      721 gaagcttttt aggccctgct aaggtagaca cgactttaat gaattttaca aaacttgcta
      781 atgtactgcc taaaaagtca acagttcgtg gatcaatcaa gcgatcttct tggtcaaatg
      841 cttcttttgc ttttcccaat aaaaattcat ttccgggcat cactaatgca ttcacgcctg
      901 gtgcgtccaa aatttggcgc aagtgtaatt gcgcgcgtga agttccttga tcgtagtaag
      961 aagcacccac aaccattaca ggtttatttt caagtggatg gattttaaat gatagccatt
     1021 caagtgcact tttcaatcct gctggaaccg tatgattgtg ttctggcgta gcaataatta
     1081 ccccatcagc cattaagatt ttgcgattta gctgttgaat agcaggacta tttgtttgat
     1141 catcatcttg attgaacaga ggtaaatctt tgatttcaat gatttctaaa ttaaatagtt
     1201 catgatattg tttttggata aatgttaata atttacggtt atatgaaagt tcagctgctg
     1261 atccgacgat acctattaat ttcatctttg tttgctcccc tcgattaaat cgtttcccat
     1321 gaaaagcttt cggcttcacg tttatttgct tggtgcgcat gttgcaattg actagtgatt
     1381 gtgacaaaga gtaagaaatc tttgaatagt tcacgtaatt ttgcgacttg ttcttcatca
     1441 atgagtcgtc cttgctcatc aaaggcttgg agtgaatgac ccaataaaaa ttctgagctt
     1501 ggcataattc gtgcctttaa ctctggtgaa tccaatattt ggcgcaaatg tgcttgtgcg
     1561 cgtgaagaac ctaacgtgcc ataagacgct ccggtaatca tcactggttt atccacaaac
     1621 ggaaaaatac catatgaaag ccaatttaat gcgttaatca atgaagcagg aaccgcatgg
     1681 tcatactctg gtgtgctgat gattacgcca tctgcttgtt caatttttcc agcaatttct
     1741 ttaacaacat ttggtagttc tgtgttttca ggtttgttaa atagtggcag ctccttgatt
     1801 tcgacaattt cgatttctgc ctcatttaca aaataatttt tcataaactg taataattca
     1861 cggtttgttg accgcgttga atttgttcct actaatccaa taaatttcat cttcattctc
     1921 tttcctttct aacatcgatt tctgactgtt cgcacgtact aaattttttc aaatcctttt
     1981 gtatacaaga attgattctc ttttgttaca actgctccct cgatacccgg ctgttcttca
     2041 agcgctttca acacttctaa agaagatgct ccaaataaac gtgtagtcca gatttctcca
     2101 tctacagaag aatcagacaa aatcgtcagg cttgctacgt catttttaac tggaaagcca
     2161 gttttcggat caatgatgtg atgatacact gacgaaccat tttgtagata acgttcgtag
     2221 attccagagg taactaaaga ttggttgttt agtttaacca ccgcaagcga ctcttcacgt
     2281 gaacctttag gatttcgcaa accaatacgc caagcaccat ccgaacgtct tggtgattca
     2341 ccaaaaacaa cgatatttcc tcctaagtta actaaagcaa acgctgcatt tcgctctcgg
     2401 caatgtgtaa ttaatttatc tgcaatgtaa ccttttgcta acgcccccaa gtcaatcatc
     2461 attcctggtt tggttaaaaa aacggttttc attttttcat ctaattgaat ttggtgtgga
     2521 tccgttagca gaagtaactc agttatctct gcttttgttg gaaccaatgc atcttcaaat
     2581 ccaatgtgcc atttttgtac aaccggacca atagcaatat tcagataact atttcttgca
     2641 caactatgtg cttttcctaa tttgatgagc tcaaaaagtt caggatgtac aatcacagct
     2701 tgtcgtccag cagcttgatt gatggccatt aattcagaag ttgcatcatg tgcgctgaag
     2761 cgatgttcat aaacgtgcaa ccattcgcct aactctgcta gtaatcgact aggctcttca
     2821 tgcccaactt gtaatgtaat tgttgtcccc atcatttgcc agattttcgt ttcttcgatc
     2881 acctgattca ttttgtacct tctttacttg aactacggat tacatgagta attagttttt
     2941 atttttgtta ctgttttcac aaccagatga tacgcctgct agatttattt gtcaaccgat
     3001 tttatatatt tttttatata ataaggtaat tttaatataa aattttatat tgtaaaaaaa
     3061 caaataaggg aaacaaacaa cacatttttg tgtgttgctt atttccctct aactatcgtt
     3121 ttctatttga tggttaacct acttaaaata aatactgcaa cgacaaacgg agatcaagca
     3181 tgctttgtaa cgtattttac gataattacg actttatttt attactctat cttgctgcca
     3241 tacccgtcta tccctaatca tctttggtga attttttcaa agttagcata acaaaaataa
     3301 gtattttttc cttagttaat caatagtatc atttttttca tatgatttaa ttcatcaatt
     3361 aattttttgt ttttattaat tttagcaaaa gctagtgcat cttgaatcaa ttcgagcact
     3421 tctgccttcg acttattttt agcctgtgcg ttcttcgcta attgggcagc agctcgtgct
     3481 aagtaataag tcaaatggtt atccgaacaa atatcgattg cgtaatttag tagtgcatca
     3541 cttgtttcca aatcaccgtc ttgcgcaaaa aaaacaccac aatgaaagac gatatttaac
     3601 acccgccaga tatcttctgt tgtacgaatc ggatacgtat aaattttttc aaacactcga
     3661 ttgaaataaa cttctgcttt ttccaactct ttttctctag catagaccat tccaatacct
     3721 gtatatgcca ataaacgaaa caacgtgttg gctgtttctg ccatcaacaa ctgatcaaaa
     3781 tggaacaaca catccgtaat tggtgcattc gtaaaaatca tttcaaatcc ttttagatag
     3841 tagtaacgtg caatcagctc agactcgtca attaccttgg gatcaatggc tgctaaaatc
     3901 tgtgtcaatt gttcatattc actcgtaatc agtgaaaact ctgctttttc tagctgttca
     3961 tcaagttgtg tattgatcac gttcatcgaa gaaaaaagtt caactaacgg aagattgatt
     4021 cgattacata atttgattaa aatttttaac gaagggactt gaccattgtt ctcaaattta
     4081 cttaatgttg cttgtgtaca aattccttca gacacttctt tttgagataa gttggcagcc
     4141 tttcttgccg taataaattt ttcaatgttc attattttct ctccaatcag acctcacacg
     4201 aaaatcattt ctgtctcgat tttagcatgt tttctttggg aatgctttag gttctgttgc
     4261 aaagttttaa ataaagaata aagtccctta cggtatctat gatttaagct
//
LOCUS       DARBET010000131         4309 bp    DNA     linear   BCT 07-NOV-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4027
            SAMEA114569841-rid20112183.denovo.137, whole genome shotgun
            sequence.
ACCESSION   DARBET010000131 DARBET010000000
VERSION     DARBET010000131.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA114569841
            Sequence Read Archive: ERR12203666
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4309)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4309)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001972618.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001972618.1
            Genome Coverage       :: 132x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/07/2023 13:18:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,041
            CDSs (total)                      :: 2,975
            Genes (coding)                    :: 2,908
            CDSs (with protein)               :: 2,908
            Genes (RNA)                       :: 66
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 67
            CDSs (without protein)            :: 67
            Pseudo Genes (ambiguous residues) :: 0 of 67
            Pseudo Genes (frameshifted)       :: 26 of 67
            Pseudo Genes (incomplete)         :: 28 of 67
            Pseudo Genes (internal stop)      :: 20 of 67
            Pseudo Genes (multiple problems)  :: 6 of 67
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4309
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA114569841-rid20112183.denovo.137"
                     /isolate="VRE4027"
                     /db_xref="taxon:1352"
                     /country="Denmark"
     gene            <1..915
                     /locus_tag="SCJ86_002758"
     CDS             <1..915
                     /locus_tag="SCJ86_002758"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YSIRK-targeted surface antigen transcriptional
                     regulator"
                     /protein_id="HEG1170243.1"
                     /translation="KMNNLNVAMADRERLYNSLQNLTLYSLGDIRDILILVHYFFTGK
                     IEDLFHEPLIEYTENLSKTIEQIKIDNLLSQNYDPEIYLFLYENKILEYVKNGDIRNL
                     ENMVFNLSNGIIPSVSGDTIRSEKNYSIIVFEKLAQTSITLGMDIIEAYQSRDALIQE
                     NELAVSLPEVLKVRDSGIVYYTKEIGKTKIEHLSPLISSVVQFIGLNIYKRITVKEIA
                     NYFSVSETKLRESFKNEMHITIYNYISKRKISTAKIMLKSNHTISEVSLGLGFSDSSH
                     FSRVFKKYAGVSPKQYQLGLVDNFNNIS"
     gene            932..1366
                     /locus_tag="SCJ86_002759"
     CDS             932..1366
                     /locus_tag="SCJ86_002759"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002360344.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEG1170244.1"
                     /translation="MRLYHLRSLEELRKLDFNKKFFWEISDLSCFVESERLSNGNNSV
                     LNEYDKVVINKVKSLCEEARESISHINGSNFKQTLFNLIKLDAKISSYLFFLIHDEFM
                     DYQKLDQLIERESWEDYYVGLTFSEKLNNYKLIDYKYLSESN"
     gene            1983..>4309
                     /gene="esp"
                     /locus_tag="SCJ86_002760"
     CDS             1983..>4309
                     /gene="esp"
                     /locus_tag="SCJ86_002760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016250137.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="repetitive surface protein Esp"
                     /protein_id="HEG1170245.1"
                     /translation="MVSKNNKRVFLEKTKKRVLKYSIKKLSVGVASVLIGVGLVLGTT
                     ELVKAQDEISPSSPLETAISSVQVGDKVASGNTFQENPGYTKNYNFSDLQFSPQELTG
                     DTLKGNTIGFEVYGKHNIAASTKNWEIRLQLDERLAKYVEKIQVDPKKGIGSSRRTFV
                     RINDSLGRPTNIWKVNYIRASDGLFAGAETTDTQTAPNGVITFEKSLDEIFKEIGIDN
                     LKTDRLMYRIYLVSHQDDDKIVPGIDSTGYFLTDSDDFYNSLDVSENNPDQFKHGSVS
                     AKYEKPNTQTKDGSGSTGANGAIILDHKLTKNYNFSYSSSAKGTPWYANYKIDERLVP
                     YVAGIQMHMVQADKVTYDVSFESGTKVADLAIERREGHENYGIGSITNNDLTKLIDFA
                     NASPRPVVIRYVLQLTKPLDEILEDMKATAQVEENKPFGEDFIFDSWLSDTNKKLIQN
                     TYGTGYYYLQDIDGDGNPDDKEESGDTNPYIGKPELEEVYDVDTTVKGKVFIHELAGT
                     GHKAQLVDKEGTVLAEKTIAPNEKDGAPISDTVEFEFTGVDSSKLIAKDELKIQIVSP
                     GFDKPEEGSTVIKESPKAVDKQTVVVGFKPDAKESIRNNKNLPEDAEYSWKTEPDTSN
                     VTDSTKGIVTVKIGNRTFDVDVEFAVKASQAMENDATYVPITTTPETTVQSGKPTFDK
                     PDVPLAKDAFSISDVYNKYFGNASVDANTGIVTFTPAKGVGESEPITGTIPIKIVYQD
                     GSVGTTDLAVTVSKDIYENPGENIPAGYHKVTFTAG"
BASE COUNT         1584 a          597 c          809 g         1319 t
ORIGIN      
        1 aaaatgaaca atttgaatgt tgcaatggct gatagagaac gactctataa ttcattgcaa
       61 aatttgactt tgtattcttt aggagacatt cgagatattc tgattttagt tcattatttt
      121 tttactggaa aaatagaaga tctattccat gaaccgttaa ttgaatatac agaaaatcta
      181 agtaaaacta tcgagcaaat caaaatagat aatctgttat cacaaaatta cgatccagaa
      241 atatatctgt ttctttatga aaataaaata ctggaatatg ttaaaaatgg agatattcga
      301 aatttagaaa acatggtttt taatctgagt aatggaataa ttccaagtgt tagtggagat
      361 acgattcggt ctgaaaaaaa ttattctatc attgtttttg aaaagttagc acaaacgtct
      421 ataacattag gaatggatat aatagaagcg tatcaaagta gagatgcttt gatacaagaa
      481 aacgaattag ctgtatcgtt acctgaggtc ttaaaagtga gggattctgg gattgtttat
      541 tatactaagg aaatcggaaa aactaagata gagcatcttt ctcccttaat ttcttctgtc
      601 gttcaattca tcggcttaaa tatctataaa agaattactg taaaggaaat agctaattat
      661 ttttctgtaa gtgaaactaa attaagagaa agctttaaga atgaaatgca tattacgatt
      721 tataattata tctctaagcg aaaaatttct acggctaaaa taatgttgaa atcaaaccat
      781 actatcagtg aagtttcttt aggtttagga ttttcagatt cttcacactt ttcgagagta
      841 ttcaaaaagt acgcaggtgt atctcctaag caatatcaat taggtttagt ggacaatttc
      901 aacaacatta gctgagaggt tttattttat tatgagacta tatcatttac gtagtttgga
      961 ggagttgagg aaattggatt ttaacaaaaa atttttttgg gaaatttctg atttgagttg
     1021 tttcgtagaa tctgagcgtc taagtaatgg taataatagt gtgttaaatg aatatgacaa
     1081 agtagtaatt aataaagtaa aaagtttatg tgaagaagct agagaatcta tcagtcatat
     1141 taatggtagc aattttaaac aaacattatt taatctgatt aagctagatg ctaaaatatc
     1201 aagttattta ttctttttga tacatgatga atttatggat tatcaaaaat tggatcagtt
     1261 aattgaaaga gaaagctggg aagattatta cgtgggtcta actttttcag aaaagttaaa
     1321 caactacaag ttaatagatt ataagtattt gagtgaaagc aattaattat tcatgtagag
     1381 tactagacac tttaaatatg gacaattcaa ttataaagaa aaagaatcaa taatttaatg
     1441 aggctgtgac aaatgtcatt ttttgtttcg ctaaaaacga gtgaaaaaat gttgataact
     1501 ccaatcattc gtttaagatt ctaggaatca ttcagaaatg ctgtgaaacc gatactttat
     1561 aattataaag aaggtgattt caaattccga agaaggaatt tgaagtcacc tttttactta
     1621 tgtcacagtc tcattcagta atgcgggata aggctcctta ttccgttatt tgaatactct
     1681 gaagtttaat ctaagaaagg gtaatatttt gaaaacatgc ttcatcgcat agaaatgaac
     1741 ttaatttcag aaagaataca tacatatctt tttatatgtt ttcagctgct gaatggttaa
     1801 aaagcacaaa tatccgaaaa aaacatgaag aaatgtactt tttttgttgt aatgttcatg
     1861 aaattctcta agtaaattag ttaacataag gttagtaaga ttcctattct gtgaataata
     1921 cgtatcagat ttatctttta ttcttactag gaaagtaatt attaagggag ttgatttgaa
     1981 taatggttag caagaataat aagagagtat ttctagaaaa aacaaagaaa cgagtattga
     2041 agtacagtat caaaaaatta agtgttgggg ttgcatctgt tttgattggt gttggtcttg
     2101 tcttgggaac aactgaacta gttaaagcac aagatgaaat ttctccaagc agcccacttg
     2161 aaacagcgat aagctccgta caagtaggtg acaaagtagc ttcggggaat acttttcagg
     2221 agaatcctgg ttatacgaaa aattataact tttcggattt acaatttagt cctcaagaac
     2281 taactggaga cacactaaaa ggcaatacaa ttggatttga agtttacgga aaacataata
     2341 ttgcagcatc aactaaaaac tgggaaatcc gtcttcaatt agatgaacga ttagctaaat
     2401 atgtagaaaa aattcaggtc gatccaaaga aaggaattgg atcttctaga cggacctttg
     2461 taagaattaa tgattcgctg ggtagaccta caaatatttg gaaggtcaat tatattcgtg
     2521 caagtgatgg attatttgct ggggcagaaa caaccgatac gcaaaccgct cctaatgggg
     2581 taattacgtt tgaaaagagc ctagatgaaa tttttaaaga gataggcata gacaatctca
     2641 agaccgatcg tttaatgtat cgaatctact tggtaagtca tcaagacgat gataaaattg
     2701 taccgggtat tgatagtacg ggctactttt taacagattc agatgatttc tataatagct
     2761 tagatgtatc tgaaaataat cctgaccagt ttaaacatgg atcagtaagt gcaaaatatg
     2821 agaaacctaa tactcaaaca aaagatggtt caggatccac aggtgctaat ggagctataa
     2881 tcttagatca taagttaacg aaaaattata atttctcata ttcatcttcc gcaaagggca
     2941 cgccttggta tgctaattac aaaattgatg aacgattagt tccatatgta gcgggtatac
     3001 agatgcacat ggttcaggct gacaaagtaa catatgatgt ttcgtttgaa tccggaacaa
     3061 aagtagctga tttagcaatc gagcgacggg aaggtcatga aaattatggc ataggttcaa
     3121 taactaataa tgatttaact aaacttatcg attttgctaa tgcaagtcca cgtccagtcg
     3181 ttattcgata tgttttacaa ttgactaagc cattagatga aatcttagaa gatatgaaag
     3241 caacagcaca agttgaagaa aataaaccat ttggtgaaga tttcatcttt gattcttggt
     3301 tgtcggatac gaataaaaaa ttaatccaga acacttatgg aacaggttat tattatttgc
     3361 aagatattga tggtgatgga aaccctgacg ataaagaaga gagcggagac acgaatccat
     3421 atatcgggaa acctgaatta gaagaagtat atgatgttga cacaacagtt aaggggaaag
     3481 tattcatcca cgagttagcg ggaacaggtc acaaagccca acttgttgat aaagaaggta
     3541 ctgtattagc agaaaaaact atcgctccaa atgaaaaaga tggggctcca atttcagata
     3601 ctgtagaatt tgaatttacg ggtgtagatt caagtaaatt aatcgcgaaa gatgaattaa
     3661 aaatccaaat cgtttctcca ggttttgata aaccagaaga aggttcaacc gttattaagg
     3721 aatcaccaaa agcggttgat aaacaaaccg tggtagttgg atttaaacca gatgctaaag
     3781 aatcaattcg gaataataaa aacttacctg aagatgcaga gtattcatgg aaaacagagc
     3841 ctgatacttc taacgttact gatagtacga aaggtattgt aactgttaag atcggaaatc
     3901 gaactttcga cgtggatgta gagtttgctg taaaagcttc tcaagctatg gaaaatgatg
     3961 caacatacgt acctataaca acaactccag aaacgacagt tcaaagtgga aaacctacat
     4021 ttgataaacc agatgttcct ttagctaaag atgccttttc aatttcagat gtctataata
     4081 agtacttcgg taatgcaagt gttgatgcaa acactggtat tgttacattc actccagcta
     4141 aaggtgtagg agaatcggag ccgattactg gaacaattcc tattaaaatt gtttaccaag
     4201 atggttctgt aggcacgacc gatttagcag taactgtaag taaagatatt tatgaaaatc
     4261 caggagaaaa cattcctgca ggctaccaca aagtaacctt caccgcagg
//
LOCUS       DARBET010000132         4187 bp    DNA     linear   BCT 07-NOV-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4027
            SAMEA114569841-rid20112183.denovo.138, whole genome shotgun
            sequence.
ACCESSION   DARBET010000132 DARBET010000000
VERSION     DARBET010000132.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA114569841
            Sequence Read Archive: ERR12203666
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4187)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4187)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001972618.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001972618.1
            Genome Coverage       :: 132x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/07/2023 13:18:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,041
            CDSs (total)                      :: 2,975
            Genes (coding)                    :: 2,908
            CDSs (with protein)               :: 2,908
            Genes (RNA)                       :: 66
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 67
            CDSs (without protein)            :: 67
            Pseudo Genes (ambiguous residues) :: 0 of 67
            Pseudo Genes (frameshifted)       :: 26 of 67
            Pseudo Genes (incomplete)         :: 28 of 67
            Pseudo Genes (internal stop)      :: 20 of 67
            Pseudo Genes (multiple problems)  :: 6 of 67
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4187
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA114569841-rid20112183.denovo.138"
                     /isolate="VRE4027"
                     /db_xref="taxon:1352"
                     /country="Denmark"
     gene            <1..134
                     /locus_tag="SCJ86_002761"
     CDS             <1..134
                     /locus_tag="SCJ86_002761"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="IS30 family transposase"
                     /protein_id="HEG1170246.1"
                     /translation="NQVNQGFISSVASKRNHIPRKSLNYQTPLEVFLSYVNGKFCLA"
     gene            326..922
                     /locus_tag="SCJ86_002762"
     CDS             326..922
                     /locus_tag="SCJ86_002762"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010737813.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TVP38/TMEM64 family protein"
                     /protein_id="HEG1170247.1"
                     /translation="MNIAASRKLINFISIIGLGLSIALTIYFINLGVFKDLNALRGLV
                     GDSIILGPIIFVFIQILQVVIPIIPGGISTAAGVLIFGPYAGFIYNYIGICIGSIIIF
                     LLGRRYGKPFILSMISDKTYNKYIGWLDNQNRFEKLFALAIFLPVAPDDALCLMAGLT
                     NMSVKRYTLIILIAKPLSIFLYSMALIYGGQYLSGLLG"
     gene            1046..2845
                     /locus_tag="SCJ86_002763"
     CDS             1046..2845
                     /locus_tag="SCJ86_002763"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326703.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerophosphodiester phosphodiesterase"
                     /protein_id="HEG1170248.1"
                     /translation="MNYLKRSLGNAWDFLKGTQAYFRDVLLMHGFILFICLPLLSSTT
                     RFILHRGAIDYLSYDNIPSIFSQHPGVLLSLIVVLMTILLLVYFEFTFLLMSVFFIKK
                     KEPISLKQLLHLTILQLKKVRPITFLFFLAYFLLILPISGLSFNSDLLSKIKIPAFIM
                     DFIFANRWIIVSSFLLVYIFLGYIGIRLIFALPEMILRDRPFRAAIRESWSLTKSRLL
                     AITGQFIVIGGTILLLSSLGYIVVILAQSMVEQFFPDYALISAVFAMTLLQGILLFNI
                     VMSTVGIFYIIVDFMDDEGFLPEIPKWFIPQAPNLRFSALKNTGLTLFAVFFGIGVCL
                     YNMDYLTSAVQTKPVTVSHRGVSTQNGAQNTLEALEKTSRDYHPDYVEMDVQETKDGH
                     FVVMHDANLRHLTGVNGTPQDLTLKELTNLTVTENGVQAPVCSFDSYLEKAHKLNQKL
                     LIEIKTSKKDSDDLVERFVHKYRKVILENHHILQSLTYQTVSDLKEAEPNFYVGYILP
                     FNIVGPPVTPADFLTMEYSTINRNFINSAHQDGKEVYVWTINDSDAMSRMMFYGVDGI
                     ITDEMGLLNESVKQTDEEITYSDKLLNFVLGVG"
     gene            complement(3123..3335)
                     /locus_tag="SCJ86_002764"
     CDS             complement(3123..3335)
                     /locus_tag="SCJ86_002764"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002308849.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEG1170249.1"
                     /translation="MVKAEVGRSYICKPIGVSHELIGCIERTYINTAVVYVESYDQRD
                     YQLINESKYRMLVKFCDISAPAELVS"
     gene            3797..3946
                     /locus_tag="SCJ86_002765"
     CDS             3797..3946
                     /locus_tag="SCJ86_002765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293931.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEG1170250.1"
                     /translation="MTFTKTNMQYRVAFDTTSNRFMAIDAQNENNVAFGITIEQAVRA
                     LNESR"
BASE COUNT         1243 a          719 c          730 g         1495 t
ORIGIN      
        1 tcaaccaagt caatcaagga ttcatctcat ccgttgcgtc caaaagaaac catatcccta
       61 gaaaatcact aaattaccaa acaccattag aagttttttt gagttacgta aatggaaagt
      121 tttgtctcgc ttaatttgac aaataatatt aaaaaaaaca agttattaaa taagtcttta
      181 gttggatgtt tttttgagat caatctttta tacttatgat gaacttacaa aattgtttgt
      241 tttctgtcat atcaaaaaat ggcgcagtta gttgcttttc tagtaaagta tagttaacaa
      301 agaacctcta aggagttgat gaataatgaa tattgctgcc tctcgaaaac tgataaattt
      361 catatccatt atcggattag gcctatcaat cgcgctgacg atttatttta ttaatttagg
      421 tgttttcaaa gatttgaatg cgttaagagg attagtaggt gattccatca ttttaggacc
      481 gattattttt gtcttcatcc aaatcctgca agtagttatt ccaatcattc ctggagggat
      541 cagtactgca gctggtgtcc tgatatttgg cccttatgct ggatttatct ataactatat
      601 tggaatctgc attggttcga ttatcatttt tctgctaggg cgtcgttatg gaaagccatt
      661 catactttcc atgattagcg ataagacata taacaaatat attggctggc tggataatca
      721 aaaccgtttt gagaagctgt ttgctctagc tatttttctt ccagttgctc ctgacgatgc
      781 gttatgtttg atggctggat tgacaaacat gtcagtgaaa cgatatacac taatcatttt
      841 gattgctaaa ccactgtcga ttttcttata tagtatggcc ttgatttatg gaggtcaata
      901 tctaagtggt cttcttggat aatatttatt ttgtaaaatt agtcattgat tgacaatgtt
      961 aaatctggga aagggtctgc aaaacacact gcaccctttc ctttttcatt agatttgcgc
     1021 tacaatgtga agagcaggtg ataggatgaa ttatttgaaa cgtagcttag gtaatgcttg
     1081 ggacttttta aaaggaacac aagcttattt ccgtgatgtc ttgctgatgc atggctttat
     1141 tttatttatt tgtttaccct tattgagcag tacgacccgt tttattttac accgaggggc
     1201 aattgactat ttgtcttatg acaatattcc ttcaattttt tctcaacatc ctggggttct
     1261 actctcgtta atcgtagttt tgatgacgat tttactgtta gtttatttcg aatttacttt
     1321 tcttttgatg agtgtttttt ttattaaaaa gaaagaacca atctctttga agcaactctt
     1381 acacttgacg atacttcagc tgaagaaagt gagacccatt acttttttgt ttttccttgc
     1441 ttatttttta ttgattctac cgatcagcgg attaagtttt aattctgatc tactgtcaaa
     1501 aataaaaatc cccgctttca ttatggattt tatttttgct aatcgttgga tcatcgtcag
     1561 ttccttttta ctcgtttata ttttccttgg ctatattggg attcgactga tttttgcttt
     1621 accggaaatg attctacgag atcgtccttt tcgagcagcc attcgggaaa gctggtcatt
     1681 gaccaaatcg cgtcttttgg ccatcacggg ccaatttatt gtgattggcg gtacgatttt
     1741 actcctttct tctttaggat atatcgttgt tattttggca caaagtatgg ttgaacaatt
     1801 tttcccagac tatgcattga tcagcgctgt gtttgctatg acgcttttac aagggatttt
     1861 attattcaat atcgtgatgt ctaccgtagg gattttttat atcattgttg atttcatgga
     1921 tgacgaaggc ttcttgcctg agatccctaa gtggtttatc ccacaagccc ccaacttgcg
     1981 tttctctgcg ctgaaaaaca ctggtttgac tctttttgca gtttttttcg gtattggcgt
     2041 ctgtctttac aatatggatt atctgacttc agctgttcag acaaaaccag tgactgtctc
     2101 tcacagaggt gtgagtaccc aaaatggggc gcaaaatact ttagaagcac ttgaaaaaac
     2161 aagcagagac tatcatcctg attatgtaga gatggatgtt caggaaacaa aagatggtca
     2221 tttcgtagtg atgcatgatg caaatcttcg tcacttaaca ggggtaaatg gaacaccaca
     2281 ggatcttaca ttgaaagaat tgacaaatct tacagttact gaaaacggag tgcaagcccc
     2341 tgtttgttct tttgattctt atttggaaaa agcacataag ctaaaccaaa aattacttat
     2401 tgagatcaag acctctaaaa aagattcgga tgaccttgtc gaacgatttg tccataaata
     2461 ccgcaaagtt atcttagaga accatcatat tttgcaaagt ttgacgtacc aaaccgtttc
     2521 agatttaaaa gaagcagaac caaactttta tgtgggctac atcttacctt tcaatattgt
     2581 tgggccccct gtgacgcctg ctgattttct gacaatggaa tatagtacga tcaaccgtaa
     2641 cttcattaat tctgcccacc aagacggaaa agaagtatat gtatggacaa tcaacgattc
     2701 agatgctatg agtcgcatga tgttttatgg tgtggatggt atcataaccg atgagatggg
     2761 actactaaat gaaagtgtga aacaaacaga tgaagaaatc acttattcag ataaactgct
     2821 aaattttgtt ttgggagtcg gctaatcaaa aatcgttaat gaaaaaagtc tttatacaac
     2881 aaaaaacgca taacctgcca ccgcaggtat atgcgttttt tgtttgacgc tcataatgtg
     2941 tgcttaactt tctcttgatt ccttcatcgc acgaacaata gctaaaatag caggaattgc
     3001 agagatcact gcaagaataa ttcccatttt ccctgaactt tttctttttg gcatatcttc
     3061 tcgctccctt ctgtctttat ttatattata agggagttct tgcgaattct caaaaatact
     3121 gtttaagaaa ccaattcagc aggtgcgcta atatcacaga attttacgag catgcgatac
     3181 ttgctttcat tgataagttg atagtctcgt tggtcataac tttcaacata cacgacagcc
     3241 gtgttgatat atgtacgttc aatacagccg attagttcat gagatactcc gatcggtttg
     3301 catatataag atcttcctac ttctgctttt accatttcat tccctctttt ctttattttt
     3361 attttgtttt ttctttattt tataataaca aattaccttg tatttgaaaa cgtttttatc
     3421 cattttttta ttttttattt tgagtatctt tctgcttttt agataactat tttttaacac
     3481 gatcttagac gttgtgacaa aaacgtttgt ttatattcct atgttgttcg tgcgtttttt
     3541 tctttatatt gctaagttat ttataaagag gtgagcatat gatttatatt caagcaatag
     3601 tatttgtaac cttgatgatt gggctactta tcggattaaa agaaaatagc taaaccattt
     3661 aagaaaaaat aaataaggaa aatgataaat gctttttaat tgcatcaaaa aacgtacgat
     3721 gctaaacttg gtataagttc gaaagtttaa aggaaacttt caagaactaa attacattac
     3781 aaaggagatg aattttatga cgtttacaaa aacaaatatg caataccgcg tagcttttga
     3841 tacaacttct aaccgtttta tggcgattga tgcacaaaac gaaaataacg ttgcgtttgg
     3901 tattacaatc gaacaagcag tccgagcatt aaacgaatct agataaggag tgaaccattc
     3961 caaatggcga ctgatgagat ctcggaaaat caataaaaga cgtttgtaac ggtaatccgc
     4021 gacaaacgtc tttttgtatt cattacatat atttggaaat tgtctctctt tatctctaga
     4081 cctaaacaaa aacacaagtt ctaatatcaa ccatctcgtt ttttatgaca attttgttag
     4141 ctgtttcgat gataatattc cgttattatc tgtataatat ttaatat
//
LOCUS       DARBET010000133         4139 bp    DNA     linear   BCT 07-NOV-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4027
            SAMEA114569841-rid20112183.denovo.139, whole genome shotgun
            sequence.
ACCESSION   DARBET010000133 DARBET010000000
VERSION     DARBET010000133.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA114569841
            Sequence Read Archive: ERR12203666
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4139)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4139)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001972618.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001972618.1
            Genome Coverage       :: 132x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/07/2023 13:18:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,041
            CDSs (total)                      :: 2,975
            Genes (coding)                    :: 2,908
            CDSs (with protein)               :: 2,908
            Genes (RNA)                       :: 66
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 67
            CDSs (without protein)            :: 67
            Pseudo Genes (ambiguous residues) :: 0 of 67
            Pseudo Genes (frameshifted)       :: 26 of 67
            Pseudo Genes (incomplete)         :: 28 of 67
            Pseudo Genes (internal stop)      :: 20 of 67
            Pseudo Genes (multiple problems)  :: 6 of 67
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4139
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA114569841-rid20112183.denovo.139"
                     /isolate="VRE4027"
                     /db_xref="taxon:1352"
                     /country="Denmark"
     gene            complement(249..1754)
                     /locus_tag="SCJ86_002766"
     CDS             complement(249..1754)
                     /locus_tag="SCJ86_002766"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326472.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing histidine kinase"
                     /protein_id="HEG1170251.1"
                     /translation="MIKPKKSEKRELKGPSLTIKWAFASSFFIFVVFTIFAVITYKSS
                     INLIVAKERNNVERTISEVASRLANSDEELTLVNTYRNLTKATNDGDEVVDHTSLVEG
                     NLMKIDSFIAELGQPSMDLYVYDLDENLIFKTHENSRNLIQTTRKVPTIVTLEGKTGF
                     LSIQPIYSKGTREKIGYAQSFYELSSFYDIRNKLLLTLIVLEVVSLILSSILGFFLSS
                     YFLKPLKVLRDTMDTIRKDPQSDIHMPEIDTNDELADLAEIFNEMLDRMRLYIEQQEQ
                     FVEDVSHELRTPVAIIEGHLSLLNRWGKDDPEILEESLSASMQEISRMKSLVQEMLDL
                     SRAEQVDVHYANETTKGKEVTYQVYNNFKILYPDYVITLDDDLTKEVTLQIYRNHFEQ
                     LIIIILDNAVKYSTTRKEVHISISSTLNEFEIAIQDFGEGIPKEDLNKIFNRFYRVDK
                     ARARTKGGNGLGLSIAKQLVENYKGRILAESVVGQGTIFRIFIPIAKAEDK"
     gene            complement(1751..2437)
                     /locus_tag="SCJ86_002767"
     CDS             complement(1751..2437)
                     /locus_tag="SCJ86_002767"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010763438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HEG1170252.1"
                     /translation="MSNILIIEDEKNLARFVELELKHEGYNAEVHYNGRTGLDAALNN
                     EWDAILLDLMLPELNGLEVCRRVRQVKNTPIIMMTARDSVIDRVSGLDHGADDYIVKP
                     FAIEELLARLRALLRRIDIEGDKNVAKQTTITYRDLTIEKENRVVRRGNEVIELTKRE
                     YELLLTLMENVNVVLARDVLLNKVWGYETEVETNVVDVYIRYLRNKIDVPGEESYIQT
                     VRGTGYVMRS"
     gene            complement(2630..4051)
                     /gene="gndA"
                     /locus_tag="SCJ86_002768"
     CDS             complement(2630..4051)
                     /gene="gndA"
                     /locus_tag="SCJ86_002768"
                     /EC_number="1.1.1.44"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342997.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-dependent phosphogluconate dehydrogenase"
                     /protein_id="HEG1170253.1"
                     /translation="MSKQQIGVVGMAVMGKNLALNIESRGYSVALFNRTGAKTTAVVE
                     EHPDKNFKATYTIEEFVESIEKPRRILLMVKAGPATDATIQELLPHLDKGDILIDGGN
                     TFFKDTMRRNEELANSGINFIGTGVSGGEEGALKGPSIMPGGQKEAYALVAPILEQIS
                     AKAEDGAPCVTYIGPNGAGHYVKMVHNGIEYGDMQLIAESYDLMKNILNLSVEEMADI
                     FKEWNQGELDSYLIEITADILTRKDDEGTGKPVVDVILDAAGNKGTGKWTSQSALDLG
                     VPLPLITESVFARYISAYKEERVQASKILSRTNDFEFTGDKKELVEKIREALYFSKIM
                     SYAQGFAQLRVASKEFDWDLPFGEIAKIWRAGCIIRARFLQKITDAYDKNPEIENLLL
                     DDYFVEITKKYQQSVRDVVALAVQAGVPVPTFSSAIAYFDSYRAERLPANIIQAQRDY
                     FGAHTYERVDKEGIFHYSWYHEE"
BASE COUNT         1146 a          868 c          698 g         1427 t
ORIGIN      
        1 tatagcaatc tctttataaa attgcaatag aaaattccct atttttattt attattttta
       61 aacacgaata ttacagcatt ttattgtttt ttcgttcatc taaaagatac ttacatttaa
      121 ttaacggatg ttattctatt taattgttcg ctagtttttt tataatgaca tccctgaatt
      181 tctcacttgc ttgtattttg taccttattc tttgttggat atataaaaaa acaagtaaag
      241 cacatgcttt acttgtcttc tgcctttgcg ataggaataa agattctaaa aattgtccct
      301 tgccctacta cactttctgc taagatacgt cctttatagt tttcaaccag ttgtttagcg
      361 atagacaacc ccagaccatt tccacccttc gttctagctc ttgctttatc cactcgataa
      421 aaacgattga agatcttatt aaggtcttct ttgggtattc cttcgccaaa gtcctgaatc
      481 gcgatctcaa attcattcag agtggaagaa atagagatat ggacttcttt tcgtgtagtt
      541 gaatacttta ctgcgttatc caatatgata atgattaatt gttcaaagtg attacgataa
      601 atttgcaggg tcacttcttt tgtcaaatca tcatctaaag tgatcacata gtctggatat
      661 aatatcttga aattattata aacttgatag gtgacttctt ttcctttagt tgtttcattc
      721 gcataatgaa catctacttg ttctgctctt gataagtcta gcatctcttg aactaagctt
      781 ttcatacggc tgatttcttg catgctggcg cttaatgatt cttctaatat ctcaggatca
      841 tctttccccc aacgatttag cagactaaga tgcccttcaa taattgcaac aggtgtcctt
      901 aactcatgag atacgtcttc cacgaattgc tcttgttgtt caatatacaa acgcatacgg
      961 tcaagcatct cattgaatat ttcagctaaa tctgccaatt catcatttgt gtcgatttca
     1021 ggcatgtgga tatctgattg cggatcttta cgtatagtat ccattgtatc tctgagtacc
     1081 ttgagtggtt ttaagaaata agaagataaa aagaaaccta aaatactact taagatcaaa
     1141 gaaacaactt ctaaaacaat aagagtcaat aatagcttat ttcgaatatc atagaaagaa
     1201 gataattcat agaagctttg cgcatagccg atcttttctc ttgtaccttt agaatatata
     1261 ggctggatag ataaaaagcc agtttttcct tctagtgtaa cgatcgttgg aactttacgt
     1321 gtagtctgaa tcaggtttcg actgttttca tgggttttaa aaatcaagtt ctcgtccaaa
     1381 tcataaacat aaagatccat tgatggctgc cctagctcag ctataaagga atcgatcttc
     1441 attaagtttc cttcgaccag tgaagtatga tctacgacct catctccgtc atttgtcgct
     1501 ttcgtcagat tacgatacgt atttactaat gtcagttctt catccgagtt agccaaacga
     1561 gaagctactt ctgaaatcgt acgctctaca ttgtttcttt cttttgccac aatcagattg
     1621 atagatgatt tataggtaat gacagcgaaa attgtaaaca caacgaatat gaagaaagaa
     1681 ctcgcaaagg cccacttgat tgtcaaggag ggccctttca gttctctttt ttccgatttt
     1741 ttcggcttta tcacgagcgc atcacataac ctgttccgcg tacagtttgg atatagcttt
     1801 cctccccggg aacgtcaatt ttgttgcgaa gataacgaat atatacgtcc actacgtttg
     1861 tttcaacttc tgtttcgtat ccccacactt tatttagtaa aacgtcacgt gctaatacca
     1921 cgttcacgtt ttccattaaa gtaagcagca actcgtattc tctttttgtt agttcaatta
     1981 cttcatttcc gcgacgtacg acacggtttt ctttttcgat agtcaaatcg cgataagtaa
     2041 tagtagtttg tttagccaca tttttatcgc cttcaatatc gattcgacgc aacaatgcac
     2101 gaagacgtgc taataattct tcgatagcaa atggtttgac gatgtagtca tctgctccgt
     2161 ggtcaagtcc agatacacga tcgatcacag aatctcttgc agtcatcata atgattggtg
     2221 tatttttgac ttggcgtaca cgtctgcaga cttctaaacc gttcaattct gggagcatca
     2281 gatccaacaa tatggcatcc cactcattat ttaatgccgc atctaaacca gttcggccgt
     2341 tgtagtggac ttctgcgtta tacccttcat gcttcaactc tagctcgaca aatcgtgcca
     2401 agttcttttc atcttcaatg attagaatat tgctcattta ttattttatc ctttctattc
     2461 tactggcttt ttaaaaactc tatcattata ccgataatat gcttaaaaat ctatagtcat
     2521 tttatcatgg atagcaattt taaaaaagag ctgaagtcta ctcataacaa tcgacttcag
     2581 ctctttcgtc ctctttttat tttctcattt cctaacagcc gaaagactat tattcttcat
     2641 ggtaccatga gtaatggaag attccttctt tgtcgacacg ctcataggta tgtgcgccaa
     2701 agtaatcgcg ttgggcttgg attatatttg caggcagacg ttctgctctg tatgaatcga
     2761 agtaagcgat ggctgatgag aaagtcggta caggtacgcc agcttggaca gccaaggcaa
     2821 cgacgtctct gactgattgc tgatattttt tcgtgatttc aacgaagtaa tcatctagca
     2881 ataaattttc gatctcagga tttttgtcat aggcatccgt aattttttgc aagaatcgag
     2941 ctcggatgat acatccagcg cgccagattt ttgcgatttc accaaatggc aagtcccaat
     3001 caaattcttt tgaagcgaca cgtaattgag caaatccttg ggcataactc atgattttac
     3061 tgaaatacaa cgcttctcgg attttttcga ccaattcttt tttgtctccc gtgaactcaa
     3121 aatcgttcgt acgagacaaa atcttgcttg cttgtacgcg ttcttcttta taagcagaaa
     3181 tatagcgggc aaatacagat tcagtgatca atggaagtgg cacacctaag tcaagcgcgc
     3241 tttggcttgt ccatttacct gttcctttat tcccagctgc atcaaggatc acatctacta
     3301 ctggtttacc tgttccttca tcgtctttac gtgtcagaat gtcagcagtg atttctatca
     3361 aatagctgtc taattcgcct tgattccatt ctttaaagat atcagccatt tcttctactg
     3421 aaaggtttaa aatattcttc attaagtcat aagattcagc aatcagttgc atatctccat
     3481 actcaatacc gttgtgtacc attttcacat aatgtccggc tccatttggg ccaatatatg
     3541 tgacacatgg tgctccgtct tctgcttttg cagaaatttg ctctaagatt ggtgcaacta
     3601 gcgcataggc ttctttttgt ccaccaggca tgattgaagg tccttttaaa gctccttctt
     3661 caccaccgga aacgcctgta ccgataaagt tgatgccaga gttggctaac tcttcattac
     3721 gacgcatcgt atctttaaag aatgtatttc ctccgtcaat caaaatatcg cctttatcaa
     3781 ggtgaggaag taactcttgg attgttgcat ctgttgccgg tcctgctttg accattagca
     3841 agatccgacg tggtttctcg atagattcta caaattcttc gattgtatat gttgctttga
     3901 aatttttatc tgggtgttct tctactacag cggttgtttt agcccctgta cgattgaata
     3961 aagccactga atagccgcgg ctttctatgt tcaaggctag atttttaccc ataacggcca
     4021 taccgacaac gccgatttgt tgttttgaca tatgaactcc tcctacatac gggatttctc
     4081 cctaatctct tacattatag cttaacacgg ctacattgca aactaaagaa caacatatt
//
LOCUS       DARBET010000134         3925 bp    DNA     linear   BCT 07-NOV-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4027
            SAMEA114569841-rid20112183.denovo.140, whole genome shotgun
            sequence.
ACCESSION   DARBET010000134 DARBET010000000
VERSION     DARBET010000134.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA114569841
            Sequence Read Archive: ERR12203666
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3925)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3925)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001972618.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001972618.1
            Genome Coverage       :: 132x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/07/2023 13:18:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,041
            CDSs (total)                      :: 2,975
            Genes (coding)                    :: 2,908
            CDSs (with protein)               :: 2,908
            Genes (RNA)                       :: 66
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 67
            CDSs (without protein)            :: 67
            Pseudo Genes (ambiguous residues) :: 0 of 67
            Pseudo Genes (frameshifted)       :: 26 of 67
            Pseudo Genes (incomplete)         :: 28 of 67
            Pseudo Genes (internal stop)      :: 20 of 67
            Pseudo Genes (multiple problems)  :: 6 of 67
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3925
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA114569841-rid20112183.denovo.140"
                     /isolate="VRE4027"
                     /db_xref="taxon:1352"
                     /country="Denmark"
     gene            complement(573..1130)
                     /locus_tag="SCJ86_002769"
     CDS             complement(573..1130)
                     /locus_tag="SCJ86_002769"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294436.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEG1170254.1"
                     /translation="MRNLSNRNKILIIIVVIAVFHLGTNAVLSRIILGPKPPRPEITR
                     GEFDFRLEYEVDGERIVIEDTIVALFDGFSADAGSMAWYRTWRLHLASDRRSRNILLD
                     ELEDGRRIYYVPESANYSMGDVQKKREPNPNWYPFNGVTIEYPRNKTPEIGAKFISGL
                     EDLYDRFGIRLISWEHDPPIENRFE"
     gene            complement(1309..1920)
                     /locus_tag="SCJ86_002770"
     CDS             complement(1309..1920)
                     /locus_tag="SCJ86_002770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEG1170255.1"
                     /translation="MNSNRGNKMEYNGSMRIVQIIFWITSGIVIIGGVFLMLPSLIFP
                     FFALISPKIPEPEITYGEFPFRIEYELDGQINIIEDTVIAEFNGCEFSAGSMKRERRW
                     RSRLASGREDLPFGDDLGIYFSRGSAQYYMGENVQSMSLKPHIALRNLEEGFKREVDF
                     ILGESGYIRTVLNFGEAQEVLTQYGITLINWEISEPIVNNFGD"
     gene            complement(2033..2290)
                     /locus_tag="SCJ86_002771"
     CDS             complement(2033..2290)
                     /locus_tag="SCJ86_002771"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629330.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEG1170256.1"
                     /translation="MRAVHVNPTIEITMIGHSKGGAEAANSVAINTNAIIFNPATVNL
                     SAYELDSNTYTANMVAYVVEGDALNVVFGGVYTYWRINHVR"
     gene            complement(2887..3165)
                     /locus_tag="SCJ86_002772"
     CDS             complement(2887..3165)
                     /locus_tag="SCJ86_002772"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287830.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEG1170257.1"
                     /translation="MEKHNITGSMYVDDQWFISTDEVKIAAIRRDYLQKIHKIKDPNG
                     KKARLIITLITSNGKKLKLAGSYDNVKKFEHWYLQKDSISLNKQFEKN"
     gene            complement(3199..3450)
                     /locus_tag="SCJ86_002773"
     CDS             complement(3199..3450)
                     /locus_tag="SCJ86_002773"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321467.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEG1170258.1"
                     /translation="MKEKPINKWSLYKNSMFGVVIFGVCAGLFLFILLQTVTLFSSNP
                     TYGWRDSIALGMFAGGYGFLLLFSILTLSPIHGLIQLWK"
BASE COUNT         1195 a          799 c          520 g         1411 t
ORIGIN      
        1 ttattccgaa taaatggtgt ccaaaagctt gcatctgcca gattatctca tttcttatga
       61 caagctaagc aaattatctc caccaaataa tttattcata tttttgactt gcgcatacta
      121 actaattctt aaattttcat acaaaatttc tcaatttccc ttaacgagta atatttatgt
      181 acggtgattc tcactagtat cccaatcact ttttaagtta tcccgatttc aattctactt
      241 caaaaatagt tgcttttatg tatgctgtta gttgattaga tggtcatttg ttgagctagg
      301 tcgtttattt taaacaagtt gtttctttag cttataccta tctcgtctac caaacgcaag
      361 atggcaaaca caaatgcata cgcatctgta tttgccactt tgattcgtat aaagctaaca
      421 actattaact cctctcagct aatagttgct ctctttttta ttcagattca tttttgctga
      481 tcacagccta gtatttcaat ggttttgaac actaaaccaa attgttccat tacaacattc
      541 actggctgat gaaatggatt agtagatttc gattattcaa agcgattttc tattggtgga
      601 tcgtgttccc aactaataag acggattcca aacctatcgt acagatcttc taaaccactt
      661 ataaattttg cacctatttc aggtgtttta ttgcgtggat attctattgt tacgccatta
      721 aatggatacc aattaggatt aggctctctt tttttctgta catctcccat agaatagtta
      781 gcactttctg ggacataata tattcttcga ccatcctcta attcatctaa taagatattt
      841 cgtgatcgtc tatcacttgc taaatgtaga cgccacgtgc gataccatgc catgctacct
      901 gcatctgcag aaaatccatc aaataatgca acaattgtat cttctatcac aatcctttcc
      961 ccatctactt catattctaa tctaaaatca aactcccctc gggtaatttc tggtcttggt
     1021 ggcttaggac ccaatattat tctacttaaa accgcattcg ttcccagatg aaacacagct
     1081 ataacaacaa taatgattaa tattttgttt ctatttgata aatttctcat tattcgcaca
     1141 tcacctctac attctcacat attagttcgt aatactcctt taatatcaaa atatctactg
     1201 gtcgtaataa tattcgctat gaattcctac tactcgtata agaaaggcca aatcatcagc
     1261 ttttacttgc tgatgatttg gcttgtttct atttatcgta attgttttct aatcaccaaa
     1321 attgttaaca atcggttcag atatttccca gttgattaag gtaattccgt attgtgtcaa
     1381 aacttcttgc gcttctccga aattaagtac agttcttatg tatccacttt ctcctaatat
     1441 aaagtctacc tctcttttaa aaccttcctc gagatttcta agggctatat gtggttttaa
     1501 tgacattgat tgtacatttt cccccatata atattgcgca cttcctcgac taaaatagat
     1561 tcctagatcg tccccaaaag gtagatcttc tctaccacta gctaatctac ttctccatct
     1621 tcgttctctt ttcatagaac ctgcgctgaa ctcacacccg ttaaactcag ctatgacagt
     1681 atcttcaatt atgttaatct gtccatctag ttcgtattca atcctaaatg gaaattctcc
     1741 ataggtgatt tccggttcgg gtattttagg tgagattaat gcaaagaaag gaaatattaa
     1801 gcttggcaac atcaaaaaaa ctcctcctat gatcacaatt cccgatgtaa tccaaaatat
     1861 aatttgtaca attctcattg atccattgta ttccatttta ttccccctat tactattcat
     1921 atccgcctat ctatatcgat cttccaatgc agagataatt gcttccatac cttgattttt
     1981 tactgagtta cctatgcgag atatcattct ccactaaggt gcatcatatt gtctatctaa
     2041 catgattaat tctccaatag gtgtatactc ctccaaaaac aacatttaat gcatctccct
     2101 cgacaacata agctaccatg tttgctgtat atgtgtttga gtctaattca taagcagata
     2161 aatttactgt cgctggatta aatataatcg cattcgtatt tatagctact gaatttgctg
     2221 cttcagcacc accttttgaa tgtccgatca ttgtgatttc aatagtagga tttacgtgga
     2281 cagccctcac cgtttaccct gaccattata gaaatcgctt acttttttga actacgcaac
     2341 aaaggataca atgctcctaa cttcactgtc tccatttttg tccactaagg aatataaaaa
     2401 ataggcatta attcatttaa ttcggtcaca acacgttacc ttttaaatca ttatactgta
     2461 tctcatacat tattatcaat aaactattat aaaaatgcaa aaaatgcttt atcaatctat
     2521 tttgtaattg gtcataatat cattgaaatc ataccctgta tcgatttttc ctatgaatct
     2581 cgtctatttg tgattgacga aaaagaggca agacagacac tggaatggta attttaccca
     2641 ctattttgct ccaactattt gtaatcgtac ccacttttga aataccgaga tgatcatttt
     2701 aattgatatg ctgattccca agcaaaaccc caagaaatta aatgctctat cgcatctagc
     2761 ttcttgggtt ttgcttatat aaattccaac atttaaattt tttatgctgt tcggtctcag
     2821 aaatgccgtt tttgcttata tcacaatatc aagatgtcaa attattcgtg ttgccgatga
     2881 ctttgattaa tttttttcaa attgtttatt aagactaatg ctgtcttttt gcagatacca
     2941 atgttcgaac tttttaacat tatcatatga tcctgcaagt tttaacttct taccattaga
     3001 tgttatcaaa gttataatta accttgcctt tttcccatta ggatctttta ttttatgaat
     3061 tttctgcagg taatctctcc taatggcagc tatttttacc tcatcagtgc tgataaacca
     3121 ttgatcatcc acatacatac tgcctgttat gttgtgtttt tccatttctt ccgagaacga
     3181 aaagtttaag gctcgttctt acttccaaag ttgaatgagt ccatggattg gacttagtgt
     3241 gagaattgag aacaacaata agaatccata tcctcccgca aacatcccta aggctatact
     3301 gtctctccat ccataagtag gattagaaga gaaaagagtc accgtttgta ataatatgaa
     3361 aaggaataat ccagcacaaa caccaaagat aacaactcca aacattgaat ttttatacaa
     3421 tgaccattta tttataggct tttctttcat tttacttccc cctactgttc tgttcacacg
     3481 gaatgaattc aaagttcata agccctcctc tttgaacata tgaatacgac ttgctaacta
     3541 tactttaaaa aagcttgttt taagcggtta taggcgagtt gtaatctcta ttcatctcat
     3601 tagcaaataa cttgccgaaa agtctactga aattaaagca gatagtggaa gaattattta
     3661 gccaggatag acactatttg tgaaacacat gtctttactt ttgaaattct atgatgaaaa
     3721 gccttggaat acagtagctc ttaattggta atactttttc cattataaat aacctctttt
     3781 cattttatat ttatcccctc taaaaataat atctaatcta atattttaaa cttatatttc
     3841 cctcgctgtg ctaatagagc attactaaat aaatagaaac tagaaaaaag cattacgcct
     3901 gccagaataa accaattatt tgtat
//
LOCUS       DARBET010000135         3875 bp    DNA     linear   BCT 07-NOV-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4027
            SAMEA114569841-rid20112183.denovo.141, whole genome shotgun
            sequence.
ACCESSION   DARBET010000135 DARBET010000000
VERSION     DARBET010000135.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA114569841
            Sequence Read Archive: ERR12203666
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3875)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3875)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001972618.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001972618.1
            Genome Coverage       :: 132x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/07/2023 13:18:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,041
            CDSs (total)                      :: 2,975
            Genes (coding)                    :: 2,908
            CDSs (with protein)               :: 2,908
            Genes (RNA)                       :: 66
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 67
            CDSs (without protein)            :: 67
            Pseudo Genes (ambiguous residues) :: 0 of 67
            Pseudo Genes (frameshifted)       :: 26 of 67
            Pseudo Genes (incomplete)         :: 28 of 67
            Pseudo Genes (internal stop)      :: 20 of 67
            Pseudo Genes (multiple problems)  :: 6 of 67
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3875
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA114569841-rid20112183.denovo.141"
                     /isolate="VRE4027"
                     /db_xref="taxon:1352"
                     /country="Denmark"
     gene            158..2257
                     /locus_tag="SCJ86_002774"
     CDS             158..2257
                     /locus_tag="SCJ86_002774"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TFIIB-type zinc ribbon-containing protein"
                     /protein_id="HEG1170259.1"
                     /translation="MKKCPNCGSKKMEQNTICPDCGHQITAEDQQIKESIEETNTDAA
                     MQDKSTEDAVFPDTELNDPIEWSELKDLPLESVMELFEKAEPEDEEPPAHDQVTNIEK
                     NEQKKATVHETSEESGKNQEETLESKQKRRVAELKEIVDNEEENSILSAYIKAHREDT
                     KEEHAKELLKMISEKIAQENKVEPEKEQETEKDSDRENATGKETVKIKDVKTDITEKT
                     EVFTEKQLEKEKTNKLPEAEKKEILENTDKKSTEPDEINSESDAENQSVSKEGTDLEA
                     KIEKEENQPKLFEAEAKSITIVPDTEKTHQESNLEKTDPDKAQKKKSKKVPYLVLAAV
                     LLLGGGSWAYYDHHQKVEAEVAAEAKRQQQMEELHEKLAAFYTDDHHQFIRADMIHQD
                     LSKLKNDLSSLKNEKGYSELETTYQDIQSKIRDIQKVNEWFIAPAIVDDHLADDIKLK
                     ADKEIQAIETDNTDFGKLIQQAQTEAKNQYAELQTAKEKTAIVFKDGKVVDSATRDQY
                     KTAKEAVEKVKNTDLSAQLNEQLKQVDKMLTDKEKKEEEEKKKAEEAKKAAEEQAKQA
                     AQAAAAAQAAQQAAVAQAAQQEAASSTPSSSNSANQPIKSARASDIADASNPAWTWAP
                     GVQSKVIATCIQRGYIVEGGYRLEKARIENGEGYYNLYATSTKSALMKGIGESALPFY
                     IVTINCKTGWFGGNGSN"
     gene            2453..3316
                     /locus_tag="SCJ86_002775"
     CDS             2453..3316
                     /locus_tag="SCJ86_002775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339421.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein"
                     /protein_id="HEG1170260.1"
                     /translation="MNDLLATIFTGIIIDENEKNYFVQKNGQTFGLSKSEGEHKIGEA
                     VEGFGYQNQKHENRITTQIPKSRIGHYAFGIVTATRKDLGAFVDIGLPDKDFVVSLDE
                     LPTMTELWPKKGDRLMISLRVDAKDRIWGSLADEKIFKSLSKYGTEEMKNKDVIGTAY
                     RLKLTGTYVLTEDFYLGFIHPSERYNEPRLGEQIKGRVIGVRPDGVLNLSLKPRAYEA
                     ISDDAQMILTILERSADQQINFTDKSDPEEIMKAFGISKGAFKRALGNLLKQGLITQE
                     NGVTKLVKKSN"
     gene            3421..>3875
                     /locus_tag="SCJ86_002776"
     CDS             3421..>3875
                     /locus_tag="SCJ86_002776"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290152.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional repressor"
                     /protein_id="HEG1170261.1"
                     /translation="MVMNAISAMKKTKQQLHEAGFKLTPQREATLLVLLENEKEHLSA
                     EEIFFLVKKKSPEIGLATVYRTLEILTELKIVDKVSFNDGLARYDLRKEGAKHFHHHL
                     LCLNCGTIEEVEEDLLLEVEQVVEERYHFLVKDHRLTFHGICQECYEKE"
BASE COUNT         1543 a          646 c          782 g          904 t
ORIGIN      
        1 agcttgagac tacgcagtct caagcttttt tttctatagg aaggaaattc attcattatt
       61 tttaagtaaa tcctaaaaat aagctattgg cgtgacgtat agaatatgat agattaaatg
      121 gtagcgtaaa cctagtaaag gagccgatcg ctaagtgatg aaaaaatgtc caaattgcgg
      181 atcgaaaaaa atggagcaga atactatctg tccagactgc ggtcatcaaa taactgcaga
      241 agatcagcaa ataaaagaat caattgaaga aacaaataca gatgcagcta tgcaggataa
      301 atcaactgaa gatgcagtct ttcctgatac agaattaaac gatccaatcg aatggtcgga
      361 attgaaagat ttacctctag aatctgtaat ggaattattc gaaaaagcag aaccagaaga
      421 cgaagaacca cctgctcacg atcaagtaac taatatagag aaaaatgaac agaagaaagc
      481 aactgttcat gagacttcgg aagaatcagg aaagaatcaa gaagaaacgc ttgaatcaaa
      541 acaaaaaaga agagtagctg aactgaaaga aatcgtcgat aatgaggaag aaaattcgat
      601 actatccgct tatatcaaag cacatcgtga agatacaaaa gaagagcacg cgaaagagct
      661 gctcaaaatg atcagtgaaa aaattgcaca ggaaaacaaa gtagagccag aaaaggaaca
      721 agaaactgaa aaagactctg atagagaaaa cgccacaggt aaagaaacag taaaaataaa
      781 agacgtaaaa acggatatca cagaaaaaac tgaagtattt actgaaaaac aattagaaaa
      841 ggaaaaaacg aataaactgc cggaagcaga gaaaaaagaa atactcgaaa acacagacaa
      901 aaagtcaaca gagcccgatg aaataaatag tgaatccgat gctgaaaatc aatctgtaag
      961 caaagagggt actgatcttg aagcaaaaat agaaaaagaa gagaaccagc ctaagctgtt
     1021 tgaagcagaa gcgaaatcaa taacaatcgt gccagatact gaaaaaacac atcaagaatc
     1081 aaacttagaa aaaacagatc cagataaagc gcaaaagaaa aaatcaaaaa aagtacctta
     1141 tctcgtttta gcagcagtac ttctactcgg tggaggaagc tgggcttatt atgaccatca
     1201 tcaaaaagta gaagccgaag tagcagcaga agcaaaaaga cagcaacaaa tggaagaact
     1261 tcatgagaaa cttgctgcct tttatacaga tgatcaccat caatttattc gtgcagatat
     1321 gattcatcaa gatttatcaa aactaaaaaa tgatttatcc agtcttaaga atgaaaaagg
     1381 atattcagag ttagaaacaa cttaccaaga tatccaatct aagatccgag atatccaaaa
     1441 agtgaatgag tggttcatag cacctgccat cgtcgatgat catctagcag atgacatcaa
     1501 gttgaaagca gataaagaga tccaagcaat cgaaacggat aacacagatt ttggaaaatt
     1561 gattcaacaa gcacaaacag aagctaaaaa tcaatatgct gaattgcaaa cagctaaaga
     1621 aaaaacagct atagtcttta aagacggaaa agtagtggat tcagcaacac gcgaccaata
     1681 caagacggca aaagaagcag tagaaaaagt gaaaaataca gatctatctg ctcagttgaa
     1741 tgaacaattg aaacaagtag ataagatgct cactgataaa gagaaaaaag aagaagagga
     1801 aaagaaaaaa gcagaagaag cgaaaaaagc agctgaagaa caagcaaaac aagccgctca
     1861 agcagcagct gcagctcaag ccgctcaaca agctgcggtc gcacaagcag cacagcaaga
     1921 agcggcatcc tcgacccctt cctcttcaaa ctcagcgaat cagccaatca aatctgccag
     1981 agcaagtgat attgccgatg catctaatcc agcatggacg tgggcaccag gtgtccaaag
     2041 caaagtgatt gctacatgta tccaacgtgg atatatcgtt gaaggaggtt acagattaga
     2101 aaaagccaga atcgaaaatg gggaaggcta ttataatcta tatgccactt caacaaaatc
     2161 tgcattgatg aaaggcattg gagaaagtgc tttgccattt tacatcgtaa ccatcaactg
     2221 taaaactgga tggttcggag gaaatgggag caattaattt taggcaaatg aaagaggctg
     2281 tgagcctgcc gtttaacacc gtttattcga aaaaggggag tatgacacaa ctgttgtcat
     2341 actccttttt tcacctttcc aaaccaaggc tcagctattc aagtagataa cattatgtta
     2401 tgatagcaaa gaaatgtatt aagaaaacct ttcactatag agggagaaaa aaatgaacga
     2461 tttattagca actatattta caggtattat tattgatgaa aatgaaaaaa actactttgt
     2521 ccaaaaaaac ggacaaacct ttggcttaag caaatcagaa ggtgaacaca aaatcggtga
     2581 agcagttgaa ggattcggtt accaaaatca aaaacatgaa aatcggatca ctacacaaat
     2641 tcctaaaagc cgtatcggtc attacgcttt tggaatcgtt actgctacac gaaaagattt
     2701 aggtgcgttt gtcgatatcg gtcttccaga taaagacttt gtcgtatctc ttgatgaatt
     2761 gccgacaatg acagagctat ggcctaaaaa aggggatcgc ttgatgattt cccttagagt
     2821 tgatgcaaaa gaccgtattt ggggcagttt agcagatgaa aaaatattca aatctttaag
     2881 caaatatgga acagaagaaa tgaaaaacaa agatgtgata ggaacagcat accgcttgaa
     2941 attaacaggt acgtacgttt taacggaaga tttctatctt gggtttatcc atccttctga
     3001 acgatataat gaacctcgtc taggcgagca gataaaaggt cgagtgattg gtgtccgacc
     3061 agacggtgta ctgaatcttt cattgaagcc aagagcatat gaagctatat cagatgatgc
     3121 acaaatgatt ttaaccattt tagaacgttc tgcagatcag caaatcaact ttacagataa
     3181 atcggatcca gaagaaatca tgaaagcttt tggtatcagc aaaggagcat ttaagcgcgc
     3241 attaggtaat cttttgaaac aaggattgat tacacaagaa aacggtgtga caaaactagt
     3301 caaaaaatct aattgagaat cccttgcatg tttttcttaa ctagcttata ataattttaa
     3361 ttaacatagt gttatatctt tttttatttg taattattat aaattgttgg ggttgagtaa
     3421 atggtcatga atgctatttc ggcgatgaaa aaaacaaaac agcagctaca tgaagcgggg
     3481 tttaagctga caccgcaaag agaagctact ctgcttgtgc ttttggaaaa tgagaaagag
     3541 catttatctg cagaagaaat cttcttttta gtgaaaaaaa agagtccgga aattggactt
     3601 gctactgttt atcgaaccct tgaaatcttg acagaattga aaattgtcga taaagtaagt
     3661 tttaatgatg gattagcccg ttatgatttg agaaaagaag gcgccaaaca tttccatcat
     3721 catttactat gcttgaactg cggcacgatc gaggaagtag aagaggatct tttacttgaa
     3781 gtggagcaag tagtagaaga acgttatcat tttttagtaa aagatcatcg gctaacgttc
     3841 catggaatct gtcaggaatg ctatgaaaaa gaaaa
//
LOCUS       DARBET010000136         3835 bp    DNA     linear   BCT 07-NOV-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4027
            SAMEA114569841-rid20112183.denovo.142, whole genome shotgun
            sequence.
ACCESSION   DARBET010000136 DARBET010000000
VERSION     DARBET010000136.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA114569841
            Sequence Read Archive: ERR12203666
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3835)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3835)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001972618.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001972618.1
            Genome Coverage       :: 132x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/07/2023 13:18:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,041
            CDSs (total)                      :: 2,975
            Genes (coding)                    :: 2,908
            CDSs (with protein)               :: 2,908
            Genes (RNA)                       :: 66
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 67
            CDSs (without protein)            :: 67
            Pseudo Genes (ambiguous residues) :: 0 of 67
            Pseudo Genes (frameshifted)       :: 26 of 67
            Pseudo Genes (incomplete)         :: 28 of 67
            Pseudo Genes (internal stop)      :: 20 of 67
            Pseudo Genes (multiple problems)  :: 6 of 67
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3835
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA114569841-rid20112183.denovo.142"
                     /isolate="VRE4027"
                     /db_xref="taxon:1352"
                     /country="Denmark"
     gene            <1..1360
                     /locus_tag="SCJ86_002777"
     CDS             <1..1360
                     /locus_tag="SCJ86_002777"
                     /EC_number="2.1.1.72"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="type I restriction-modification system subunit
                     M"
                     /protein_id="HEG1170262.1"
                     /translation="LIATIVDILGYAIAPEYLFNVLADQAKQATFQLNDLNKAFVQLA
                     STYNQFNGLFDDVDLQSKKLGTDEQQRNVTITEVIKKLNDVDVLEHDGDVIGDAYEFL
                     ISQFASEAGKKAGEFYTPHMVSDMMAQIVTLDQKERRFFSVFDPTMGSGSLMLNVRNY
                     LTHPDNVKYHGQELNTTTYNLAKMNLILHGVDAEEMNLRNGDTLNKDWPTDEPYTFDA
                     VVMNPPYSANWSADTTFLDDSRFNRYGKLAPKSKADFAFLLHGFYHLKETGTMAIVLP
                     HGVLFRGAAEGVIRQKLLEDGSIYAVIGMPANLFFGTSIPTTVIVLKKNRQTRDVLFI
                     DASREFVKGKNQNKLSEENIQKILETYAERKDVEKYAHLATFDEIKENDYNLNIPRYV
                     DTFEEEEPIDMVHVGNDIKKIRQEQQVLEKKLLEAISSLQTTPENEAWLQGALEVFKH
                     EQ"
     gene            1350..2567
                     /locus_tag="SCJ86_002778"
     CDS             1350..2567
                     /locus_tag="SCJ86_002778"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323630.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="restriction endonuclease subunit S"
                     /protein_id="HEG1170263.1"
                     /translation="MSNDTQPEIRFPGFTDDWEQRKLGDFVVDYVEKTSVQNQFPMLT
                     SSQQKGIVLQEDYFANRQVTTENNIGYFVLPRGYFTFRSRSDNDVFVFNRNDIIDRGI
                     ISYFYPVFTLKSADSDFFLRRINNGIQRQLSIQAEGTGQHVLSLKKFKNIVAMFPSEE
                     EQQKIGTFFKQLDDTIALHQRKLDLLKETKKGFLQKMFPENGAKVPEIRFPGFTEDWE
                     ERKLGELGKTQSGIGFPDAEQGGSEGTPFFKVSDMNNIGNEYEMRNANHYVSNEQIER
                     KKWKPIKDVPAIIFAKVGAAIMLNRKRLVTSPFLIDNNTMAYLFNNTWDIYFGKILFE
                     TINLPRYSQVGALPSYNSSDIENISVKVPVKDEQQKIGTFFKQLDDTIALHQRKLDLL
                     KETKKSFLQKMFV"
     gene            2580..>3835
                     /locus_tag="SCJ86_002779"
     CDS             2580..>3835
                     /locus_tag="SCJ86_002779"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334404.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I restriction endonuclease subunit R"
                     /protein_id="HEG1170264.1"
                     /translation="MKISHREEAEVEEQLIRVLGEGHNQWTYRPDLKSEEDLWVNLRQ
                     KIISNNQAELNDFPLTDKEFETIKTELLLRTKTPFDAAKWLKGENGMARITIEREDSQ
                     LGSVSLILYSNQDIGGGISTYEVVHQIAKRGSNIEARDRRFDVTLLINGLPIVQIELK
                     QVTAKDGVYQAFNQIKKYAEEGMFRNNIFSTLQLFVVSNEQTTRYFANALPKDLHPKF
                     LFSWRTKDNEKVENLYEFCKQVLNIPDAHRLIADYTIVSEDQDNKTLMVLHPYQVHAI
                     QALFIAANKHQSGYVWHATGSGKTLTSFVSTKLLARKSGIDRTIMLVDRKDLDNQTTT
                     EFTKFASEFNTGISSGNAKANSLIVGTGSAKELSETLLADANANVVIITTRQKLDAAL
                     KYAKKQEEKKGTNRFQKLMGQHIVFVV"
BASE COUNT         1320 a          660 c          800 g         1055 t
ORIGIN      
        1 tctgattgcc acaatcgttg atattttggg atatgcaatt gcaccagagt atcttttcaa
       61 cgtcttagcg gatcaagcaa aacaagcaac gttccaattg aacgatctaa acaaagcctt
      121 tgtgcaattg gcatctacct acaatcaatt taatgggtta tttgatgatg tggatctcca
      181 atcaaaaaaa ctaggaacag atgagcaaca acgaaatgtg acgattaccg aagtcatcaa
      241 gaaattgaat gatgtagatg ttttagaaca cgatggggac gtgatcgggg atgcgtatga
      301 gtttctgatc agtcaatttg cttcagaagc agggaagaaa gccggagaat tttacacacc
      361 tcatatggtt tctgatatga tggcgcaaat cgtaacgctc gatcaaaagg aacgacgatt
      421 ctttagtgtt tttgatccaa cgatgggttc aggttcattg atgttaaacg tacgtaacta
      481 cttaacacac ccagataacg ttaaatatca tgggcaagaa ctaaatacaa cgacatacaa
      541 tctagcaaaa atgaacttga ttttacatgg tgtcgacgcc gaagaaatga atctccgcaa
      601 tggggatacg ttgaataagg actggccaac agatgaacct tacacatttg atgcagtagt
      661 aatgaaccca ccttattcgg ctaattggtc agcagatacg actttcttag atgattctcg
      721 gtttaatcgt tatgggaaat tagcgccaaa atccaaagca gattttgcgt ttcttcttca
      781 tgggttctat catttgaagg aaacaggtac catggccatc gtcttaccac acggagtttt
      841 attccgtggc gctgcagaag gggtcatccg tcaaaaatta ctagaagatg gcagtattta
      901 tgctgtgatc ggtatgcctg cgaacttgtt ctttgggaca tcgattccaa cgacggtgat
      961 tgtgttgaag aagaatcgtc aaactcgaga tgtattattc attgatgcta gtcgtgaatt
     1021 tgttaaaggg aaaaatcaaa ataaactatc ggaagaaaac attcaaaaaa tcctcgaaac
     1081 ctacgctgaa agaaaagatg tcgagaaata cgctcatttg gcaacctttg atgaaatcaa
     1141 agaaaatgac tataacttaa atatcccacg ctatgtagat actttcgaag aagaagaacc
     1201 aattgatatg gttcatgtcg gaaatgacat caagaagata cgacaagaac agcaagtgct
     1261 agaaaaaaaa cttttagaag ctatctcttc cctgcaaaca acacctgaaa atgaagcgtg
     1321 gttacaaggt gcgctggagg tgttcaaaca tgagcaatga tacacaacca gaaatccgtt
     1381 tcccaggatt tacagatgat tgggaacaga ggaagttggg ggacttcgtt gtagattatg
     1441 ttgaaaagac aagtgtacaa aatcaatttc ctatgctaac ttcatctcaa caaaaaggga
     1501 tagttttgca agaagactat tttgcaaatc ggcaagtaac tacagaaaat aatattggtt
     1561 actttgtact acctagagga tatttcacgt tccgtagtcg aagtgataat gatgtgtttg
     1621 tgtttaaccg taatgacatt attgatagag ggattataag ctatttttat cctgtattta
     1681 ctttaaaatc tgctgattcc gacttcttct taagacgtat taacaatgga atccaacgac
     1741 aattatctat tcaagcagag ggaacaggtc aacatgttct gtctctaaaa aagtttaaaa
     1801 atatagtagc aatgtttcct agtgaggaag aacaacaaaa aatcggcacc ttcttcaaac
     1861 aacttgacga cacgatcgct cttcatcagc gtaagttaga tttgctgaag gaaacgaaaa
     1921 aaggattctt acaaaaaatg ttccctgaaa acggagcaaa agtgccagaa atccggtttc
     1981 ctggttttac ggaagattgg gaagagcgta agttgggaga attaggtaaa actcaatcgg
     2041 gaattggatt tcctgatgcc gagcaaggtg gttctgaagg tactccattt tttaaagttt
     2101 ccgacatgaa taacattggt aatgagtatg aaatgagaaa tgcaaatcat tatgtatcta
     2161 atgagcaaat tgagcgtaaa aaatggaagc caataaaaga tgtgccagcg attatatttg
     2221 ctaaagttgg cgcagcaata atgcttaata gaaaacgttt ggtcacaagc cctttcttaa
     2281 tagataacaa taccatggct tatttattta ataatacttg ggatatctat tttggtaaaa
     2341 tattgtttga aactataaat ttgccaagat actcccaagt aggggcattg ccttcttata
     2401 acagttctga tattgagaat atcagtgtta aggttcctgt aaaggatgaa caacaaaaaa
     2461 tcggtacttt cttcaaacag ctagatgata cgatcgctct tcatcaacgt aagttagatt
     2521 tactaaaaga aacgaaaaaa agcttcttac aaaagatgtt tgtctaggag ggaagaagaa
     2581 tgaaaattag tcaccgtgag gaagcagaag tcgaagaaca gttgattcgt gtacttggag
     2641 aagggcataa tcagtggacg tatcgtccgg atctaaaatc agaagaagac ctttgggtca
     2701 atttacgcca aaaaattatc tcaaataatc aagcagaact gaatgatttt cctttgaccg
     2761 ataaagaatt tgaaacgatc aaaacggaat tactgctgcg aacgaaaacc ccatttgatg
     2821 ctgcgaaatg gttaaaaggt gaaaatggga tggctcggat cacgattgaa cgggaagatt
     2881 cgcaattggg ttctgtctca cttatccttt attccaatca agacattggt ggaggaatct
     2941 ctacttatga agtggtccat caaattgcaa aacgtggaag taacatagaa gcacgggatc
     3001 gacgttttga cgtcacctta ttaatcaatg gattaccgat tgtccaaatc gagctgaaac
     3061 aagtaactgc aaaagatggt gtttatcagg catttaacca aatcaaaaaa tatgcagaag
     3121 aaggtatgtt tcggaacaat atcttttcga ccttacagtt gtttgtggtt tccaatgaac
     3181 aaaccactcg gtattttgca aatgccttac cgaaagactt gcaccctaag tttttattta
     3241 gttggcgaac gaaagacaat gaaaaagtag aaaacttata tgagttttgt aaacaagtgt
     3301 taaatatccc ggatgctcat cgtttgattg cggattatac aatcgtcagt gaagatcaag
     3361 acaataaaac cctcatggtg ttacatcctt atcaagtaca tgcgattcaa gccttgttta
     3421 ttgccgcaaa taaacatcaa tcagggtatg tgtggcatgc gactggttca ggaaagacgt
     3481 tgactagttt tgtatctact aagcttttag cacgaaaatc ggggatcgat cgaacgatca
     3541 tgttagtgga ccgcaaagat ttagataatc aaaccaccac agagtttaca aaatttgctt
     3601 cggaatttaa tactgggatt tcttccggga acgccaaagc gaatagcttg atcgttggaa
     3661 cgggaagtgc caaagaatta agtgaaaccc tgttggcaga tgccaatgca aatgttgtaa
     3721 tcattacaac ccgtcaaaaa ctagatgcgg cgttgaaata tgcaaagaaa caagaagaga
     3781 aaaaaggaac caatcgcttc caaaaattaa tgggccaaca tatcgtgttt gtagt
//
LOCUS       DARBET010000137         3712 bp    DNA     linear   BCT 07-NOV-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4027
            SAMEA114569841-rid20112183.denovo.143, whole genome shotgun
            sequence.
ACCESSION   DARBET010000137 DARBET010000000
VERSION     DARBET010000137.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA114569841
            Sequence Read Archive: ERR12203666
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3712)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3712)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001972618.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001972618.1
            Genome Coverage       :: 132x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/07/2023 13:18:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,041
            CDSs (total)                      :: 2,975
            Genes (coding)                    :: 2,908
            CDSs (with protein)               :: 2,908
            Genes (RNA)                       :: 66
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 67
            CDSs (without protein)            :: 67
            Pseudo Genes (ambiguous residues) :: 0 of 67
            Pseudo Genes (frameshifted)       :: 26 of 67
            Pseudo Genes (incomplete)         :: 28 of 67
            Pseudo Genes (internal stop)      :: 20 of 67
            Pseudo Genes (multiple problems)  :: 6 of 67
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3712
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA114569841-rid20112183.denovo.143"
                     /isolate="VRE4027"
                     /db_xref="taxon:1352"
                     /country="Denmark"
     gene            complement(161..2158)
                     /gene="tkt"
                     /locus_tag="SCJ86_002780"
     CDS             complement(161..2158)
                     /gene="tkt"
                     /locus_tag="SCJ86_002780"
                     /EC_number="2.2.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340613.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transketolase"
                     /protein_id="HEG1170265.1"
                     /translation="MFDKIDQLGVNTIRTLSIEAVQKANSGHPGLPMGAAPMAYALWT
                     KHLKVNPTTSKNWADRDRFVLSAGHGSAMLYSLLHLAGYQVTIDDLKQFRQWDSKTPG
                     HPEVHHTDGVEATTGPLGQGIAMAVGMAMAEAHLAATYNKENFNVMDHYTYAICGDGD
                     LMEGVSQEASSMAGHMKLGKLIVLYDSNDISLDGPTSKAFTENVGARYEAYGWQHILV
                     KDGNDLEAISKAIEEAKAETDKPTLIEVKTVIGYGAPKEGTSAVHGAPLGEDGIKMAK
                     EVYGWEYPDFTVPEEVAARFHQTMIEEGQKAEDAWNEMFANYKKAYPELAQQFEDAFD
                     GKLPENWDAELPTYEVGSSQASRVSSKEVIQELSKAIPSFWGGSADLSGSNNTMVAAD
                     KDFTPEHYEGRNIWFGVREFAMASAMNGIQLHGGTRIYGGTFFVFVDYLRPAVRLAAI
                     QHTPVVYVLTHDSVAVGEDGPTHEPIEQLASVRCMPGVQVIRPADGNETRAAWKVAME
                     TTDAPTILVLSRQNLPVLPSTKEVADEMVKKGAYVLSPSQGETPEGILIATGSEVDLA
                     VKAQKELAEKGKDVSVVSMPSFDLFEKQSSEYKESVLPKSVKKRVAIEAAASFGWERY
                     VGTEGATITIDHFGASAPGTKILEEFGFTVENVVNTYNQLS"
     gene            complement(2260..2511)
                     /locus_tag="SCJ86_002781"
     CDS             complement(2260..2511)
                     /locus_tag="SCJ86_002781"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF896 family protein"
                     /protein_id="HEG1170266.1"
                     /translation="MLSPEKIERINELARKKKENGLSKAEEQEQINLRKEYLEAFRSG
                     MRHHIEGMKVVDPEGNDVTPEKLKEIQKKKGLHNRNDNF"
     gene            2658..3281
                     /gene="lexA"
                     /locus_tag="SCJ86_002782"
     CDS             2658..3281
                     /gene="lexA"
                     /locus_tag="SCJ86_002782"
                     /EC_number="3.4.21.88"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010751062.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional repressor LexA"
                     /protein_id="HEG1170267.1"
                     /translation="MARQTESRQIEVLRYIHEQVTQKGYPPTVREIGEAVQLSSTSTV
                     HGHLARLEKKGFIQRDPTKPRAIELTKQGLEKIGIRPTTIPVLGVVTAGEPILAVEDA
                     SDFFPIPPNLSSEEGSLFMLTIRGDSMINAGILDGDNVIVRKQETAQNGDIVIAMTAE
                     DEATCKRFYKEKNFFRLQPENDLLEPIILDQVSILGRVVGLYRSHIY"
     gene            3461..3679
                     /locus_tag="SCJ86_002783"
     CDS             3461..3679
                     /locus_tag="SCJ86_002783"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297436.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEG1170268.1"
                     /translation="MKSMRRLGLVLTVLFLGIIIGNFGIRPALLVFVPLFTLWFMLWD
                     EKKYRQSERRRIHEDHFYHEPYYRSRQS"
BASE COUNT         1025 a          796 c          688 g         1203 t
ORIGIN      
        1 tcaagaaaca caaactaatc cgaagaattc tttgttttgt catttaaatg taacatagcc
       61 gaaaacgaaa agtaatattg atttattctt ctaacacata aagaacagct gtctccaact
      121 tgcagacagc tgttcttatc agtcaaacaa ttgcttggat ctatgatagt tgattgtacg
      181 tattcacgac attttctact gtaaatccaa attcttctaa aattttagtg ccaggggcag
      241 atgcaccgaa atgatcgatt gtaatcgttg cgccctctgt tcctacgtag cgttcccatc
      301 caaatgatgc agcagcttcg atagccacac gtttcttcac tgatttagga agtacacttt
      361 ctttatattc agaagattgt ttttcaaaga ggtcaaagct tggcatagaa acaacagaaa
      421 catctttgcc tttttcagcc aattcttttt gagctttcac agccaaatca acttcagaac
      481 ctgttgcaat caaaatacct tctggtgttt ctccttgtga tggagaaaga acatacgcgc
      541 cttttttcac catttcatct gctacttctt ttgtagaagg caatactgga aggttttgac
      601 gactcaatac aaggatagta ggcgcatctg ttgtctccat cgctactttc caagcagcac
      661 gtgtttcatt tccgtctgcc ggacgaatca cttgaacacc aggcatgcag cgaacagatg
      721 ctagttgttc aatcggttcg tgcgttggtc cgtcttctcc gacagcaacc gagtcatgtg
      781 ttaagacgta aacaacagga gtatgttgga ttgctgctaa acgaactgct ggtcttagat
      841 aatcgacaaa gacaaagaat gttccaccgt agatacgagt tccgccatga agttggatac
      901 cattcatagc cgaagccatt gcaaattcgc ggacaccgaa ccagatgttg cgtccttcat
      961 aatgttcagg agtgaaatct ttatctgcag ccaccattgt attattggaa cctgacaagt
     1021 ccgctgaacc accccagaag cttggtatag ctttcgataa ttcttggatg acttctttac
     1081 ttgaaacacg gcttgcttga ctgcttccaa cttcataagt tggcaattct gcatcccagt
     1141 tttctggtag tttgccatca aaagcatctt caaattgttg tgctaattca ggataagctt
     1201 ttttataatt ggcaaacatt tcattccatg catcttcagc tttttgtcct tcttctatca
     1261 tcgtttgatg gaagcgggca gctacttctt ctggaacagt gaaatctgga tactcccaac
     1321 cataaacttc tttagccatc ttgatgccat cttcaccaag aggagcacca tgaacagcag
     1381 atgttccttc tttaggagca ccatagccga tgactgtttt tacttcgatc aatgttggtt
     1441 tgtctgtttc agcttttgct tcttcaatcg ctttagagat tgcttctagg tcatttccgt
     1501 ctttcactaa gatatgttgc cagccatatg cttcatagcg agcaccaacg ttttctgtga
     1561 aagcttttga agttggaccg tctagtgaaa tatcgtttga atcgtataaa acgatcaatt
     1621 tccctagttt catatgacct gccatcgaac ttgcttcttg agatacccct tccatcaagt
     1681 ctccgtcacc gcatattgca tacgtatagt gatccatcac attaaagttt tctttgttat
     1741 acgttgctgc aaggtgcgct tctgccattg ccataccgac agccattgca atcccttgtc
     1801 ctaatggccc tgtagtggct tctacaccat ctgtatgatg tacttcagga tgtcctggtg
     1861 ttttagaatc ccattgacgg aattgtttca aatcatcgat tgtcacttga tagccagcta
     1921 aatgaagcaa gctatacaac attgctgacc catgtcctgc cgaaagaacg aaacggtcgc
     1981 ggtcagccca gttttttgat gtcgttggat ttactttcag gtgttttgtc cataacgcat
     2041 atgccatagg tgctgcgccc atcggcaatc caggatgtcc tgagtttgct ttttggactg
     2101 cttcaatact taatgtacga attgtgttaa cgccaagttg gtcaattttg tcaaacaaaa
     2161 taacccctcc tattaattta ggtttttttt acctttattt cttacacttt cattctataa
     2221 gatgtcacac gaaaaagcaa tctttttcag tattttttac taaaagttgt catttcggtt
     2281 gtgtaagcct tttttctttt ggatttcttt taatttttcc ggggtcacgt cattgccctc
     2341 aggatcgacg actttcattc cttcgatatg atgacgcata ccggaacgaa aagcttccaa
     2401 atactctttg cgcaagttga tttgttcttg ttcttcggct tttgagagac cattttcctt
     2461 ctttttacgt gcaagttcat tgatccgctc tattttttca ggtgacagca tgctcagccc
     2521 tccttttact ctccttcata ttatataaaa actgaaaaaa aaacaactag ataaactaaa
     2581 taaaatacga acattcgttt gatttagcgc tttttatatg gtactattta tttatcaagt
     2641 gaaagaaggt gtaccttgtg gcccgacaaa ctgaatcaag acaaatcgaa gtcttacgat
     2701 atatccatga acaagtaacc caaaaaggat acccgccgac tgtccgtgaa atcggggaag
     2761 ctgttcagct ttcttctact tctactgttc acggtcatct tgcacggctt gaaaaaaaag
     2821 ggtttatcca aagagatccg acgaaacctc gtgcaatcga gttgacaaaa caaggtttag
     2881 aaaagattgg gattcgtcca acaactattc cagtacttgg cgtcgtaaca gctggagaac
     2941 ccattttagc agtagaggat gcttcagact tctttccaat ccctcctaat ctttcttccg
     3001 aagaaggcag tctctttatg ctgacaattc gtggagatag tatgatcaat gctgggatat
     3061 tggatggtga caacgtgatc gttcggaagc aagaaactgc tcaaaatggc gatattgtca
     3121 ttgcaatgac tgccgaggac gaagcgactt gcaaacgatt ctataaagaa aaaaactttt
     3181 tccgtctgca gcctgaaaac gacttgctcg aaccaatcat cttagatcaa gtatctatat
     3241 taggacgagt agtaggactt tatcgtagtc atatctatta attgctacta attaaaagaa
     3301 aactttttaa aaagagtttg agacgttggt ctcaaactct tttttcgccc gggttcttct
     3361 tctttcaaat ttttatcact tccctcttta caaaccgaac aaatattcgt atactaaata
     3421 tacgaacaat tgttcgcata aattaaagag gtgagaattc atgaaatcaa tgagacgtct
     3481 tggcttagta ttaactgttt tgtttttagg tattattatt ggtaatttcg gcatacgtcc
     3541 agctttactt gtgtttgttc cgttatttac tttgtggttc atgttgtggg atgaaaagaa
     3601 gtaccgtcaa tctgagcggc gcagaattca tgaagatcat ttctatcatg agccctacta
     3661 cagatccagg caatcataat aaggattgac tgctaattat tcaaaagaca ag
//
LOCUS       DARBET010000138         3410 bp    DNA     linear   BCT 07-NOV-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4027
            SAMEA114569841-rid20112183.denovo.144, whole genome shotgun
            sequence.
ACCESSION   DARBET010000138 DARBET010000000
VERSION     DARBET010000138.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA114569841
            Sequence Read Archive: ERR12203666
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3410)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3410)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001972618.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001972618.1
            Genome Coverage       :: 132x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/07/2023 13:18:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,041
            CDSs (total)                      :: 2,975
            Genes (coding)                    :: 2,908
            CDSs (with protein)               :: 2,908
            Genes (RNA)                       :: 66
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 67
            CDSs (without protein)            :: 67
            Pseudo Genes (ambiguous residues) :: 0 of 67
            Pseudo Genes (frameshifted)       :: 26 of 67
            Pseudo Genes (incomplete)         :: 28 of 67
            Pseudo Genes (internal stop)      :: 20 of 67
            Pseudo Genes (multiple problems)  :: 6 of 67
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3410
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA114569841-rid20112183.denovo.144"
                     /isolate="VRE4027"
                     /db_xref="taxon:1352"
                     /country="Denmark"
     gene            161..1201
                     /locus_tag="SCJ86_002784"
     CDS             161..1201
                     /locus_tag="SCJ86_002784"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310953.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiator protein A"
                     /protein_id="HEG1170269.1"
                     /translation="MTDFKFFKADRVYNELFYQFPKVFIVSDEYKKMKDSTKIAYMLL
                     KARLEIAISKRQIDEEGNVYFTYTTNELCRVLNCQKQKAIAIKKELESFGLLLQKQMG
                     FNKQLGKNNPNRLYLAELKVSENDIYLLEKFDRENRENVDKSEGMKIIPTLDEKSDAE
                     SLGAQEGMKIIPCQNVDKSEGMKIIPELNNNILDTNRHNIDTEKDRLQDQLLLDNFET
                     IMTNDSIATFVPERVLNLIKTFSSSYSEAQKTVQTIHNAKKKAEIESDISIVFEELDS
                     YYVNAEQELYTTLLKAYQKLKTEKVENIQNLIFVYVKNWFIEKPIAAKVSSEKRLNYA
                     SSPSTITKDWLE"
     gene            2025..2357
                     /locus_tag="SCJ86_002785"
     CDS             2025..2357
                     /locus_tag="SCJ86_002785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323647.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein TraE"
                     /protein_id="HEG1170270.1"
                     /translation="MSYTGILSLKDICHYGKRCTATEKITKKLSTGQSKTVVQCKKYI
                     IQKDKVSEEMIYYIGKQKQIILKDPIPLKELYPTIKHVYDQNGVLIGRRKNGVLRCTA
                     KGMGRLIG"
     gene            2976..3317
                     /locus_tag="SCJ86_002786"
     CDS             2976..3317
                     /locus_tag="SCJ86_002786"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325562.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5406 domain-containing protein"
                     /protein_id="HEG1170271.1"
                     /translation="MKNYDPNIRLGTHTIKVSFQRWDYKGFVTFRRGGNCKGLDVLAL
                     DEDDLYDQTLTDNPIGFGLLPEDDEGNEWFKMTLMNDNGDELSVEDTWSYLSDYIVSF
                     EIIEFVADKEE"
BASE COUNT         1268 a          461 c          646 g         1035 t
ORIGIN      
        1 actttaaagg tagtacataa ttcattttat ttctgatttt aggtagtatt taattaaaaa
       61 acagttttaa tgcgtttgca ttttttattt atctttgcta ttttcgaaaa gagtaggtaa
      121 ttttacataa ataaaacaac gtgaaagtag aggatctaca atgactgatt ttaaattttt
      181 taaggcggat agagtttaca acgaattatt ttatcaattt ccaaaagtct ttattgtttc
      241 tgacgaatac aaaaaaatga aagattcaac taagattgcc tatatgcttt taaaagcaag
      301 attagagatc gcaatcagca aacggcaaat cgatgaagaa ggtaatgttt attttactta
      361 tacgacaaat gaactatgta gagtattaaa ctgccaaaaa caaaaagcga tagcaatcaa
      421 aaaagagttg gaatcctttg gtttattatt acaaaagcag atgggattta acaaacagtt
      481 agggaaaaat aatcctaata gactatatct agcagaatta aaagtctcag aaaatgatat
      541 ctacttactc gaaaaatttg atagagagaa tagggaaaac gttgataaat cagagggtat
      601 gaaaatcata cccaccctcg acgaaaaatc agacgctgaa tcccttgggg ctcaagaggg
      661 tatgaaaatc ataccgtgcc aaaacgttga taaatcagag ggtatgaaaa tcataccaga
      721 acttaataat aatatattag acactaatag acacaatata gacactgaaa aagaccgcct
      781 acaagatcaa ttgttgttag acaattttga gacaattatg acaaacgaca gcattgctac
      841 gtttgtccct gaacgagtat taaatttgat aaaaacattt tcttcaagtt acagtgaagc
      901 tcaaaaaacc gtccagacta ttcataatgc aaagaaaaaa gctgaaatag aaagtgatat
      961 ttcgatagtt tttgaagaac tcgatagtta ttatgtcaat gcagaacaag aattatacac
     1021 gacactgtta aaagcctatc aaaaattaaa aaccgaaaaa gtcgaaaata tccagaacct
     1081 gatttttgtc tatgtaaaaa attggtttat cgaaaaacca atagctgcta aagtatcaag
     1141 tgaaaaacgt ttgaattatg caagctcccc aagcactatt acgaaagact ggttagagtg
     1201 aaactttact gacaaagtga ttgttgcttc atggagtaac attaaaatta aaaatttttt
     1261 attattctaa ttatattacg aatattacaa aataatagct attttaatag aatttataag
     1321 ttaaaatatt atgataaaaa tcacaaacgt agccttttac gtcataagtg ttaaaagaaa
     1381 taattaattt caaatataga aaagggaacg tatgtttgca tactcctttt ttttgagtta
     1441 taataatacg cgaagagaga taatttcatt atttaaacaa gctaaaaatc ttgatattac
     1501 ttcttttcag gcaatacgaa tattattcgc ttacattttt tgtataaatt taattgtctg
     1561 ctatgcttgt gatgaattga ttttcgtttt gcacttttgg atgataaatt tggacaattg
     1621 aaaatacaaa aaaaagaaag gcggtatgaa ctcggaaggc tggtaacctt cgtcagcgtt
     1681 catacacggc attaagattt ggcccttaat gcgttaacct ttcagtgtta cggtattacc
     1741 cgcaatggat ttagtataac agatattcaa aagtgatacc actttttttg gctgtgttaa
     1801 ggttaaaaat acgtgatatt acgatgaatc tatgcctaag cataatgtta taaaaatgtc
     1861 cgtgtggctc tttgccgtat gacgaacgct gaaattagag ggaacgcatg tttggatgcg
     1921 ttccctcttt tttgtttgtc atgaacgaaa atcaattctc tatttttgaa aaataacaag
     1981 aaaaacttgt taaaaatatt taaagaaatt gggtggttta ttttatgtca tatacaggaa
     2041 ttttgagtct caaagatatt tgtcactacg gtaaacggtg taccgctaca gagaaaatca
     2101 cgaagaaact atcaactggc caaagtaaaa ccgttgtgca gtgtaagaaa tatataatcc
     2161 agaaagacaa agtctcagag gaaatgattt attacattgg gaaacaaaag caaataattt
     2221 taaaagatcc tatcccctta aaagagctat atccaacaat caaacatgtt tatgaccaaa
     2281 acggtgtact gattggtcga agaaaaaatg gagtcttgcg ttgtaccgca aaagggatgg
     2341 ggcgtttgat cggctgaatt gatcattctc ataacttgta ttttatcgtt tttcgcttgc
     2401 ttgaaaatat tgtcgagact tctaaaggaa cttagagtca ctagaaaaat aaggagtaga
     2461 cttgatctat caacaattcc cttcggggag tgacggggta ggttttaacg attggtaacg
     2521 taaaaaattg gtcaatcgag taaatgcgct tgtttggata gtggtgaaat gaatttgtgt
     2581 aaataataag cgcagagtaa acaagaggga atttagaagt tagacaagtt agaatagcgt
     2641 ctagattaat catgagcagc tactaaggga tctagacttt gacgcaaact catggaataa
     2701 ggagtaggtc ttctcgtaat ggggaggacg cacaagaaat tgtgtcgtgt gctgcagtac
     2761 atgtagatac ttgcgaggac gttgattatt ggatcaatcg agcatttacc gctgtctact
     2821 agggtttctc gtttcggacg ccataaacga gtcgaaattt tttatgtatt tttaagtaaa
     2881 cgaaaaacga taaaatatga acaagtcctg aataaagtga aaaaattaat ataaagcata
     2941 gagaaaatga aatttacgta atggaggaat gacaaatgaa aaattatgat ccgaatatac
     3001 gattgggcac acatactatt aaggtttctt tccaacgttg ggattacaaa ggatttgtga
     3061 cgtttagaag gggcggaaat tgtaaaggat tagatgtttt agcattagac gaggatgatt
     3121 tatacgatca aacattgact gataatccaa ttggattcgg cttgctacct gaagatgatg
     3181 aaggtaatga atggtttaaa atgacgctta tgaatgataa tggagacgaa ttatctgttg
     3241 aagatacttg gtcatatttg agtgattata ttgtcagctt tgaaatcatt gaatttgtag
     3301 cggataaaga agaataaaaa ctggaggaat aaattttaca gataaaggct tagaaggagt
     3361 cacgacgatt aataaaaagg tagtattacc ttaggcattg agtaaggaat
//
LOCUS       DARBET010000139         3244 bp    DNA     linear   BCT 07-NOV-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4027
            SAMEA114569841-rid20112183.denovo.145, whole genome shotgun
            sequence.
ACCESSION   DARBET010000139 DARBET010000000
VERSION     DARBET010000139.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA114569841
            Sequence Read Archive: ERR12203666
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3244)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3244)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001972618.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001972618.1
            Genome Coverage       :: 132x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/07/2023 13:18:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,041
            CDSs (total)                      :: 2,975
            Genes (coding)                    :: 2,908
            CDSs (with protein)               :: 2,908
            Genes (RNA)                       :: 66
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 67
            CDSs (without protein)            :: 67
            Pseudo Genes (ambiguous residues) :: 0 of 67
            Pseudo Genes (frameshifted)       :: 26 of 67
            Pseudo Genes (incomplete)         :: 28 of 67
            Pseudo Genes (internal stop)      :: 20 of 67
            Pseudo Genes (multiple problems)  :: 6 of 67
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3244
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA114569841-rid20112183.denovo.145"
                     /isolate="VRE4027"
                     /db_xref="taxon:1352"
                     /country="Denmark"
     gene            complement(1..352)
                     /locus_tag="SCJ86_002787"
                     /pseudo
     CDS             complement(1..352)
                     /locus_tag="SCJ86_002787"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348804.1"
                     /note="frameshifted; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(612..1160)
                     /locus_tag="SCJ86_002788"
     CDS             complement(612..1160)
                     /locus_tag="SCJ86_002788"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335394.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEG1170272.1"
                     /translation="MADIKGLDIFGNNKGLAKIKKEAEAKKKQQAITPSENKQLEVHQ
                     VQEIKTSSNVERQSRGRTKKVGAPIRKFDKVYSTKHPLKLSTLLNATSRVLVEKYETN
                     MTRDELLRKALDEYIKQNLTQEDKQDLFNDVVKELDLFREKYPIVPELDDRGEIIRTT
                     SQIKQDTEDSLRKGWGMNNKVR"
     gene            complement(1161..2018)
                     /locus_tag="SCJ86_002789"
     CDS             complement(1161..2018)
                     /locus_tag="SCJ86_002789"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334620.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ParA family protein"
                     /protein_id="HEG1170273.1"
                     /translation="MVAKKYVIGNFKGGVGKSTCAQMLGFESAVNEGKRTLIIDLDMQ
                     GNTSDVMNLTHMNFTNEEGGGEGEPIVYENTVTDVLINNLPPKEAVYKVINNLYILPA
                     DMSFEMYDDWIKDQYNDSLSQFKYMKEKLEPLFDEFDVIYLDVPPSISVYSKSAMYIA
                     DWAIVVLQTQVKSMRNAMQYLEYMDFFVKEFDTNLYVAGVIPFMLESGDAVDQEMYKQ
                     AQEIYGEHLIKNVVLKNARLKRYDGSGITMEKTKKGLLKQWDRKSHELFISILKELEE
                     HENWYAEVE"
     gene            complement(2304..2590)
                     /locus_tag="SCJ86_002790"
                     /pseudo
     CDS             complement(2304..2590)
                     /locus_tag="SCJ86_002790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292679.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
     gene            complement(2580..2909)
                     /locus_tag="SCJ86_002791"
     CDS             complement(2580..2909)
                     /locus_tag="SCJ86_002791"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005877489.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AbrB family transcriptional regulator"
                     /protein_id="HEG1170274.1"
                     /translation="MITKTRKQGNSIMLTVPKDFNVPNGVEVEAKLVENGILYEFVEP
                     QKEFYDFSEDILSDIIAEGYDKDEILVEFKNRKNKMHSSFRDIAEDTLTNSKVMTKEE
                     LATEIGL"
     gene            3131..>3244
                     /locus_tag="SCJ86_002792"
     CDS             3131..>3244
                     /locus_tag="SCJ86_002792"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012477736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS6 family transposase"
                     /protein_id="HEG1170275.1"
                     /translation="MNHFKGKQFKKDVIIVAVGYYLRYNLSYREVQELLYDR"
BASE COUNT          962 a          646 c          421 g         1215 t
ORIGIN      
        1 aatgttctta ctgcctcttc cacactaatt tcttcgttca atttcactaa taatacttga
       61 tctcgcttat tataatcgct ttcatttatc ctaagagtca aatctttttc caccataact
      121 tttacaccca ataaattttg cggtttattt ttctctgttg aaattctttg tgtagccttt
      181 aaactgacaa ttagctcatt gggtacatac ttatttgaga gtcatctaat tggctcataa
      241 tcctttcttt ttggttagta tctgcagtta cttgtttagt atgagccatg aacccaaaca
      301 tactcacccc aacaaccaat cctaaatata ttttcttttt aaactttttc atctacatct
      361 cctcctaata gttttatttt agagttagct ctattactta tttactaact caacaacaga
      421 ttatcataca cttgttaatg aaatcacaat atctcattaa aacacaccct aaaatcataa
      481 ccgttcctat gctcattttt tggatttttt aatcacgtaa ccagaaaaat aaatcccctc
      541 tatttcacgc aaatcaaaga agaactaagt aactaccgtt taaaggtagt tacttagttc
      601 ttctttgatt attatctaac tttattattc attccccacc ctttgcgcag tgagtcttcg
      661 gtatcttgtt ttatctgact ggtagttctt attatttctc cacgatcatc taattctgga
      721 acaattggat atttttctct gaacaagtct agttctttca ccacgtcatt aaacaaatct
      781 tgcttatctt cttgcgtaag attctgttta atatactcat ctaaagcttt tctaagcaat
      841 tcatctcttg tcatattagt ctcatacttt tctactaaaa ctctggaagt cgcatttaac
      901 aaagtcgaca gttttaaagg atgcttggtg gaataaacct tatcaaactt tctgattggt
      961 gcgccaactt tttttgttct tcctctactc tgtctttcaa cattggagga agtttttatt
     1021 tcttgaactt ggtgcacttc taattgctta ttttcagatg gtgtaattgc ttgctgcttt
     1081 ttctttgctt ctgcttcttt ttttattttg gcgagtcctt tgttgttccc aaaaatatca
     1141 agtcctttga tgtcagccaa ttactccacc tccgcatacc aattctcgtg ttcttccaat
     1201 tctttcaaaa tactgataaa taattcgtgg cttttgcgat cccattgttt taagagacct
     1261 ttctttgtct tctccatcgt aatacctgat ccgtcgtatc gttttaagcg cgcatttttg
     1321 agtacaacat ttttaatcaa atgttcccca taaatctctt gggcttgttt atacatttcc
     1381 tgatctactg cgtcaccgct ttccaacatg aaaggaataa caccagcaac atataaattt
     1441 gtatcaaatt ctttcacaaa aaagtccata tactcaagat attgcatcgc atttctcatc
     1501 gattttactt gggtctgtaa gacgacgatt gcccaatccg caatgtacat cgcggattta
     1561 ctatatacag aaatagaagg tgggacatct aaatagatga cgtcaaactc atcaaataat
     1621 ggttctagct tttctttcat atatttgaat tgggataatg aatcattata ttggtctttg
     1681 atccaatcat catacatttc aaaggacatg tctgctggaa gtatatagag gttattgatt
     1741 actttataga cagcctcttt aggtggaaga ttgtttatca acacgtcagt cacagtattt
     1801 tcgtaaacaa tcggttcacc ttctccgcct ccttcttcgt tagtgaagtt catatgcgtt
     1861 aagttcatca catcagaagt atttccttgc atatccaagt caattatcaa ggttcttttt
     1921 ccttcattaa ctgctgattc aaaccctaac atttgcgcgc acgtactttt acctacgcca
     1981 cctttaaaat taccgattac atatttcttc gctaccacgt tcctacctct tttcttttag
     2041 caatattcat ttgatactat tataacatat ttatttttag ttactaccaa aaaaaagata
     2101 tatatgtact accttataat taataagtaa aaggtagtac ataaataagg aaaagctgaa
     2161 atatattgat ttatagcttt cctcaaagta atgacgttat ttaatttatc gagaagcaga
     2221 gaatactcgc gactttagtc atgtagacga atcagatttt cgataaaaaa gaagccgtcc
     2281 ttcttaagaa cgacaacaaa tcactagatt aattttttca actgttcata aaagttttca
     2341 tgcgttccac ataacaatac tggaataacc ttgttttcta taatctcgta agctactcta
     2401 tatgttgtgc ccgcatattt gaacccacta gaccaaatac ctgataaatc cccagttttt
     2461 tgttcaccct tatatggatc aatcgcaatt tcatcataaa ttaaattcaa aaatttttct
     2521 ttcaatggct tctcttttag ctttttaaat acttaatgac atgttttgaa ggaacgattt
     2581 cataagccaa tttctgttgc caactcctct ttagtcatta ctttagaatt agtaagtgta
     2641 tcttcagcaa tatctctaaa ggaagaatgc attttgtttt ttctattctt aaattctaca
     2701 agaatctcat ctttatcgta gccttctgca attatatcag acaaaatatc ttcgctaaaa
     2761 tcataaaatt ctttttgtgg ttcaacaaat tcataaagaa taccattctc taccaacttt
     2821 gcttcaactt cgactccatt aggaacatta aaatctttag gtactgtcaa catgattgaa
     2881 ttgccttgtt ttcttgtttt tgtaatcatt taaatccctc atttctaaca gtatgtaatt
     2941 acttagtaaa tacattatac cacgagataa tgtatttaca agtaattact gtgactcgta
     3001 atgcgtcctt gctgaataaa tttcaaccac gtgttcatct tcatctacct tataaacggt
     3061 tctgttgcaa agttttccaa aaaatctatt ttagtgtaaa attgagaaaa aagacagaga
     3121 ggacagagta atgaatcatt ttaaaggcaa acaattcaaa aaagacgtca ttattgtcgc
     3181 tgttggttac tacctgcgtt acaatctaag ctatcgtgaa gttcaggaat tgttatatga
     3241 tcgt
//
LOCUS       DARBET010000140         3221 bp    DNA     linear   BCT 07-NOV-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4027
            SAMEA114569841-rid20112183.denovo.146, whole genome shotgun
            sequence.
ACCESSION   DARBET010000140 DARBET010000000
VERSION     DARBET010000140.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA114569841
            Sequence Read Archive: ERR12203666
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3221)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3221)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001972618.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001972618.1
            Genome Coverage       :: 132x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/07/2023 13:18:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,041
            CDSs (total)                      :: 2,975
            Genes (coding)                    :: 2,908
            CDSs (with protein)               :: 2,908
            Genes (RNA)                       :: 66
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 67
            CDSs (without protein)            :: 67
            Pseudo Genes (ambiguous residues) :: 0 of 67
            Pseudo Genes (frameshifted)       :: 26 of 67
            Pseudo Genes (incomplete)         :: 28 of 67
            Pseudo Genes (internal stop)      :: 20 of 67
            Pseudo Genes (multiple problems)  :: 6 of 67
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3221
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA114569841-rid20112183.denovo.146"
                     /isolate="VRE4027"
                     /db_xref="taxon:1352"
                     /country="Denmark"
     gene            301..585
                     /locus_tag="SCJ86_002793"
     CDS             301..585
                     /locus_tag="SCJ86_002793"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEG1170276.1"
                     /translation="MDILVRNIDPKFVATIDKRCQELTNKTGEKWSRNKYLKVLIESD
                     FDRQLTTYKKDQFDLLLDKFIAIQTENTQILSEYVYTNNQIIEMLIGGMI"
     gene            588..1004
                     /locus_tag="SCJ86_002794"
     CDS             588..1004
                     /locus_tag="SCJ86_002794"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001232859.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEG1170277.1"
                     /translation="MRKTSVQKHVTLYPSHVDVLDSIIEKNPDINNYSEAIRFLCMSY
                     EPDTNTTTTSNQAKLNAMGKEISIMTEIIAEFADLYLKDTGVLIGTNSKVYQEVKEKI
                     EAKLNPSNRNRPKNHQKETSQTVTSRNFDPTNFLKR"
     gene            complement(1141..1860)
                     /locus_tag="SCJ86_002795"
     CDS             complement(1141..1860)
                     /locus_tag="SCJ86_002795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311815.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation protein"
                     /protein_id="HEG1170278.1"
                     /translation="MDEKTELTVKYQNELNLVPLKNFNAKQMDLFFALCARMKDRGLK
                     KVRFSFEELKELSDYKMTATKAFVGDLEKLYKDMLNLTYRTEDEDSIRYFVLFTGFEI
                     NKTKKFVEVSVNPDLEYIINGLTAEFSRFELSAFTSLRSTYAKTLFRLLMQYRSTGYY
                     VVRIEEFRSLMDIPEYYQMGNIDQKVLNPALRELKKYFNDLKVTKIKAKKGNKIAKLE
                     FTFSSLTSDLPKISLRNWLEE"
     gene            2535..2630
                     /locus_tag="SCJ86_002796"
     CDS             2535..2630
                     /locus_tag="SCJ86_002796"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000493114.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system Fst family toxin"
                     /protein_id="HEG1170279.1"
                     /translation="MCFVFTNFICSLLVGLLSALFSYWLNNRNKK"
     gene            complement(2932..3177)
                     /locus_tag="SCJ86_002797"
     CDS             complement(2932..3177)
                     /locus_tag="SCJ86_002797"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311817.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEG1170280.1"
                     /translation="MRLEKEKMEKFPYEYFQNLEKLEQAIQAIKFDLTDEKLLEYKNM
                     TPERKRSLEKLMQLVNEDGGISDAAFDAWSEFHGEYN"
BASE COUNT         1074 a          599 c          503 g         1045 t
ORIGIN      
        1 aaaaaaaatg tcgtttgaaa aactaaataa atttttgatc gtgcattttt aaatttggtt
       61 atcgattaca cgtgcagcac tcccccccga attttaaaga gcataaggaa acgtgtaccc
      121 actttctaaa aaaagtgggt agataggctg tgcctatcac gtttctcaga aaaaaatttg
      181 atatcacttt ttcgccatcg tgggtagctt atgtatagca ttttggcaaa aaacagctat
      241 acaaatatag tatttgtatg gtatacataa attatacata attgcaaaag gagatttttt
      301 atggacattt tagttcggaa tattgatcca aagtttgttg caacaataga caaacgttgc
      361 caagaactca cgaataaaac aggagaaaaa tggagtcgaa ataaatatct aaaagtttta
      421 atcgaaagtg attttgaccg tcaacttacg acttacaaaa aagaccaatt cgatttattg
      481 ctcgataaat ttattgctat ccaaacagaa aatactcaaa tattaagcga gtatgtctac
      541 acaaataatc aaatcattga aatgcttatc ggaggaatga tataaccatg agaaaaactt
      601 ctgtccaaaa acatgttact ttgtacccta gtcatgttga cgttttagat tccatcatcg
      661 aaaaaaatcc agatatcaat aactactcgg aagccattcg ctttttatgt atgagttatg
      721 aacccgatac caacacgacc actaccagca atcaggcaaa actaaatgct atgggaaaag
      781 aaatttccat catgactgaa atcatcgcag aatttgctga tttatatcta aaagatacag
      841 gcgttcttat tggcacaaat tctaaagtat atcaagaagt gaaagaaaaa atcgaagcta
      901 aactaaatcc atcaaatcga aatcgaccaa agaatcatca aaaagaaacc agtcaaacag
      961 tcacttctag aaatttcgac ccaacaaatt ttctaaaacg ataaaaaaca gataggagtt
     1021 attcctatct gttttttatt tagaagcccc atacaatatg ctagaatgta ctagcaatct
     1081 tagtcggttg taagggactt gtctacttgc ttaatgtaag gggggcgagt tcttttttat
     1141 ttattcttct aaccagttac gcagagaaat ctttggcaaa tctgacgtta ggcttgaaaa
     1201 agtaaattct aatttggcga ttttattgcc cttttttgct tttatttttg tgactttaag
     1261 atcattgaag tattttttta gttctcgtaa tgcaggattt aatacttttt gatcaatgtt
     1321 acccatttga tagtattctg ggatatccat taagctccta aactcttcaa tacgaactac
     1381 ataataacca gtagaacggt actgcataag cagtcggaat agagtttttg catacgtaga
     1441 acgcaacgac gtgaaagcag ataattcaaa tctactaaat tcagctgtta aaccattaat
     1501 aatatattct aggtccggat taacactgac ttcaacaaac ttttttgttt tattgatttc
     1561 aaaaccagta aacaaaacaa agtagcgaat agaatcctca tcttcagtac ggtaagtaag
     1621 attaagcata tctttataga gcttttctaa atcaccgaca aaggcttttg tagcagtcat
     1681 cttataatca cttaactctt ttaattcctc gaaagaaaat ctaacttttt tcaaaccacg
     1741 atctttcata cgagcgcata atgcaaaaaa taaatccatc tgcttagcat tgaaattttt
     1801 cagcggtact aaattcaatt cattttgata tttgactgtt aattccgttt tttcatccat
     1861 tcctttcttc cccccttaca aataaataat aacctttaac aaccaactcg taaataggtt
     1921 ggttgttatt atacaaaagg atattagtac tcagataaaa agttgttagt actcagataa
     1981 aaagttgtta gtactcagat aaaaagttgt tagtactcag ataaaaagtt gttagtactc
     2041 agataaaaag ttgttagtac ccttctgtat ccgttgggag agtaaggctc aactgccccc
     2101 taaaaagggt tctaaaaaat ctttaaaaaa taaataaaat aaacgcgcgc gcgcgagcaa
     2161 aatttcccgc caaaaaatca cccacttttt aaaataaaca ggttcgcgca tgatattaaa
     2221 ctgccaatct agctccctat ggtcgctgta agaaggagtg atcgctacgc tctcacattt
     2281 tacttgcagc agtcgccttt aggctccttc gccaaaaata attcattagg gactcctttg
     2341 tggcgtcctg agagctcgtc taagccgttc taagacaaga ttcgataaaa actatgtcct
     2401 tcgttctaaa gcgccttaca ggcggtcagg gcaagcccta acaaccccaa ggcaataaaa
     2461 aacttaggct tatttttgct tttttattgt ataattgatt gtcgcctatc cgacagtctt
     2521 gcgactggaa ggaggtgtgt ttcgtgttca ctaactttat ttgctccctt cttgttgggc
     2581 ttttaagcgc tttgttcagt tactggttga ataaccgaaa caagaagtaa aacttaatta
     2641 aggcgactca gcccacaaaa aaagccccag agaatccgac ctctctgggg ctttcgtgtg
     2701 tttcgtgttc acttatttgc aatcaaatta taacagaaat aaatatttat ttcaaaaata
     2761 tcctacaaaa gtctaaaatc cgtaattatt tcttatatgc aacatttaca aataaagtcg
     2821 ctctgagagc tcgtattttt cgatttaaga cgttttattt agtttttggt gtatttcatt
     2881 ggtccaagct aaaaaactct tagaagcgat tttagagcca tttaaaccca cttaattata
     2941 ttctccatga aattcgctcc atgcgtcaaa agctgcgtct gaaattccgc cgtcttcgtt
     3001 cactagttgc attaattttt ccaacgatcg ttttctttcg ggtgtcatat ttttatattc
     3061 caacaatttt tcgtctgtta aatcgaactt gattgcttga attgcttgct ctaatttttc
     3121 taagttttga aaatattcgt aaggaaattt ttccattttt tctttctcca gtctcacaat
     3181 ttttctagaa ataaaatcac ttctttgccg tctatactct c
//
LOCUS       DARBET010000141         3186 bp    DNA     linear   BCT 07-NOV-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4027
            SAMEA114569841-rid20112183.denovo.147, whole genome shotgun
            sequence.
ACCESSION   DARBET010000141 DARBET010000000
VERSION     DARBET010000141.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA114569841
            Sequence Read Archive: ERR12203666
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3186)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3186)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001972618.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001972618.1
            Genome Coverage       :: 132x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/07/2023 13:18:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,041
            CDSs (total)                      :: 2,975
            Genes (coding)                    :: 2,908
            CDSs (with protein)               :: 2,908
            Genes (RNA)                       :: 66
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 67
            CDSs (without protein)            :: 67
            Pseudo Genes (ambiguous residues) :: 0 of 67
            Pseudo Genes (frameshifted)       :: 26 of 67
            Pseudo Genes (incomplete)         :: 28 of 67
            Pseudo Genes (internal stop)      :: 20 of 67
            Pseudo Genes (multiple problems)  :: 6 of 67
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3186
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA114569841-rid20112183.denovo.147"
                     /isolate="VRE4027"
                     /db_xref="taxon:1352"
                     /country="Denmark"
     gene            588..1004
                     /locus_tag="SCJ86_002798"
     CDS             588..1004
                     /locus_tag="SCJ86_002798"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325493.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NusG domain II-containing protein"
                     /protein_id="HEG1170281.1"
                     /translation="MSLKEFAEKSLLKPWDVFIILLLTVLSFLPVMIFGLQQNTATAE
                     KEAVLRVDGTEIKTFPLMAGEESNTYTYKDEHGDYNLIEIDGDRVRIKEADCDDQICV
                     RRGWATKNGETIVCLPHKLVIEVRSTDGGDEDSLIY"
     gene            1018..1584
                     /locus_tag="SCJ86_002799"
     CDS             1018..1584
                     /locus_tag="SCJ86_002799"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332226.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Gx transporter family protein"
                     /protein_id="HEG1170282.1"
                     /translation="MSKLQKMIYISLLVAQGVIIGLLENMIPYPFSFAPGAKLGLANL
                     ITIVAIFTMKKQDSFLLLWLRLFLTTLLGGTISTFLYSMSGALLSYFGMLLVKQLGPK
                     RVSIIGISAAGGFMHNVGQLLMASFIAQSWTVMLYLPVLSVLGILSGIAIGIAANYLL
                     RHVQTLQRFQKDYEQGTKQKWSPMFTKK"
     gene            1607..2593
                     /locus_tag="SCJ86_002800"
     CDS             1607..2593
                     /locus_tag="SCJ86_002800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310571.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polyprenyl synthetase family protein"
                     /protein_id="HEG1170283.1"
                     /translation="MKLHPMWENYPALSKELTETLKRMEETVRLKNKPVEQAIKETIH
                     AGGKLLRPAYQLLFSQFGPEQDRDKAIALAASIEMLHTATLIHDDIVDEADLRRGQPN
                     IRSQFGNVVSVYAGDYLFVCCFKLLSDYSTSLKSLQLNSRSMEKVLNGELGQMDDRYN
                     YELSVKEYLENISGKTAELFQLSCSVGAFESGTSERFAKKAGDIGLSIGMAFQIIDDI
                     LDYTKESQEIGKPVLEDMRQGVYSLPLIYSLQKNKPKLLPYLEKKAAMTEEDVDAVRK
                     IVEHTGGVEEARKLAASYTEKALKEIKKLPATSLRTKENLFSLTQLILDRKD"
     gene            complement(2748..>3186)
                     /gene="scm"
                     /locus_tag="SCJ86_002801"
     CDS             complement(2748..>3186)
                     /gene="scm"
                     /locus_tag="SCJ86_002801"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="collagen-binding MSCRAMM adhesin Scm"
                     /protein_id="HEG1170284.1"
                     /translation="STTTSDSTDTSASSDSTDTTSTTSDSSDSSTTTSDSTDTSASSD
                     STDTSSTTSDSSDSSTSSDSTDTSSTTSDTRHASVSFSKTSSDNRTNFNLTNKTSNDN
                     GNKSKRALPKTGSQSNNWITLAGVILLVIGLRITFMSYKKSKR"
BASE COUNT          964 a          595 c          674 g          953 t
ORIGIN      
        1 ttgttgcttt cgcattgatg aacggttccg gacgtgcgat tgggttggat cgctgggtaa
       61 ttccatggct acaacgtaca ctaggtaatt ggtggtacgg tagaccaagt tcccagtacg
      121 gcaagaaata aatagataaa gaacgttatt tgaatgcaag atttcaatag aaaggggctg
      181 agacaaaccg tcacagcctc ctttttatgt aaaattaaag gaataagatg cccgagataa
      241 gcctccatga gcaagacaca ggtgtcacaa cctctgttac aacctcttgt tataaacggt
      301 gttccagaag tagcttcttc gaaaaataag ccgaaatttc acaaaaactt gaaaagtaat
      361 tttcgtaaat tccttcttat ttcttgaagt tgcccacttc tgtcacaacc tcttgttata
      421 aacggtgttc cacctgctgc ttctcggtaa taagcccaaa gaattagaaa acatggaaag
      481 ctgttttcta ttcttcgttc ttattactca aagcaagaca caggtgtcac aacctcttgt
      541 ttttatgcta aaattgggtg agttaatctg gaaaaaggag aacaaaaatg agccttaaag
      601 aatttgctga aaaaagtctg ctcaaacctt gggatgtttt tattattttg cttttgactg
      661 ttctttcttt tttaccagtc atgatttttg gtcttcagca aaacaccgca acagcagaaa
      721 aagaagcggt actgcgtgtg gatggcacag aaatcaaaac attccctttg atggctggag
      781 aagagagcaa cacatatact tacaaagatg aacacggaga ttataacttg atcgagatag
      841 atggcgatcg agtacggata aaagaagcag attgcgatga ccagatctgc gtacgtcgag
      901 gctgggcaac gaaaaatggc gaaaccatcg tctgtttgcc acataaatta gtgatcgaag
      961 tccgttcaac agatggaggt gacgaagaca gtctgattta ttagaccttc acacaccatg
     1021 agtaaattac aaaaaatgat ttatatttcg ctacttgttg cacaaggagt gatcattggt
     1081 ttattggaaa atatgatccc ttatcctttt tcttttgcgc ctggtgcaaa actaggctta
     1141 gccaatctaa tcacgatcgt tgccattttt accatgaaaa aacaagacag cttcttacta
     1201 ctttggctac gactattcct gactactttg ctaggcggaa cgatttccac attcctttat
     1261 agtatgagtg gtgctttgct tagttatttt ggaatgttat tggtcaagca attaggacca
     1321 aaacgtgtca gtatcatcgg aattagtgcg gccggcggtt tcatgcataa tgtcggacag
     1381 cttttgatgg cttctttcat tgcacaatca tggacagtca tgttgtactt gccagtcctt
     1441 tcagttttag ggattttatc agggattgca atcgggatcg cagcgaatta tcttttacga
     1501 catgtgcaga cacttcaacg attccaaaaa gattatgagc agggaaccaa acaaaaatgg
     1561 agcccaatgt ttaccaaaaa ataatcgtca cacaaggagt ttttttatga agctacatcc
     1621 aatgtgggaa aactatcctg ctttaagtaa agaattgaca gaaacactaa aacggatgga
     1681 agaaactgtc cgcctgaaaa acaaacctgt cgaacaagcc atcaaagaaa cgatccatgc
     1741 cggcggaaag ctgttgcgtc ctgcctatca gctattgttc tcacaatttg gaccagagca
     1801 agatcgagac aaagccatcg ctttggcagc ttctatcgaa atgctccata cggccacact
     1861 gatccatgat gatatcgttg atgaagcgga tctgcgacgt ggtcagccta atatccgtag
     1921 tcagtttggt aatgtcgttt ctgtctatgc aggagactat ttatttgtct gctgttttaa
     1981 actattgtct gattactcca cttccttgaa aagtctccaa ttgaattctc gcagtatgga
     2041 aaaagtattg aatggcgaat tagggcagat ggatgatcgc tataattatg aactttctgt
     2101 aaaagaatat ttggaaaata tttcaggtaa gacagctgaa ctgttccaat taagttgttc
     2161 tgttggcgct tttgaaagtg gaacaagcga gcgcttcgca aaaaaagcag gagatatcgg
     2221 actttctatc gggatggcct ttcagattat cgatgatatt ttagattata caaaagagag
     2281 ccaggaaatc ggcaaacctg tactagaaga catgcgtcaa ggggtctatt ctttaccgtt
     2341 gatctactct ttgcaaaaaa acaaaccaaa gctactacct tatttagaga agaaagcggc
     2401 aatgacagaa gaagatgtcg acgccgtacg caaaatcgtt gaacatacag gaggcgtgga
     2461 agaagctagg aagttagcgg cctcttacac agaaaaggct ttgaaggaaa taaagaaatt
     2521 acctgccacc tctcttcgaa caaaagagaa tctcttttcc cttacccaac tgattttgga
     2581 tcgcaaagac tgaataggga aaggttcgat acgtaaataa atttctcaga aggaaatcta
     2641 cgtgtttttt gacaaattga attgtagttc ttgtatttat gataaaaagc aaaaataaag
     2701 agcaacgtcc gttagcgacg ttgctctttt ttatcttgaa tattttttta ccttttcgat
     2761 ttcttataac tcataaaagt gattcttaaa ccgattacaa gtaatataac gcctgcgagt
     2821 gttatccaat tattcgattg acttccagtt ttaggaagtg ctctttttga tttgtttcca
     2881 ttgtcattgc ttgttttatt agttaagtta aagttcgtcc tgttatccga acttgttttt
     2941 gagaaggaga cacttgcatg acgtgtatcg cttgtggtag acgacgtgtc tgtactatcg
     3001 cttgaagttg atgaatctga gctatcactt gtggtagagg atgtatctgt actgtcactt
     3061 gaagctgacg tgtccgtgct atcacttgta gtggtagatg aatctgagct atcacttgtg
     3121 gtagaggtag tatctgtact gtcacttgaa gctgacgtgt ccgtgctatc acttgttgtg
     3181 gtagat
//
LOCUS       DARBET010000142         3129 bp    DNA     linear   BCT 07-NOV-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4027
            SAMEA114569841-rid20112183.denovo.148, whole genome shotgun
            sequence.
ACCESSION   DARBET010000142 DARBET010000000
VERSION     DARBET010000142.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA114569841
            Sequence Read Archive: ERR12203666
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3129)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3129)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001972618.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001972618.1
            Genome Coverage       :: 132x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/07/2023 13:18:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,041
            CDSs (total)                      :: 2,975
            Genes (coding)                    :: 2,908
            CDSs (with protein)               :: 2,908
            Genes (RNA)                       :: 66
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 67
            CDSs (without protein)            :: 67
            Pseudo Genes (ambiguous residues) :: 0 of 67
            Pseudo Genes (frameshifted)       :: 26 of 67
            Pseudo Genes (incomplete)         :: 28 of 67
            Pseudo Genes (internal stop)      :: 20 of 67
            Pseudo Genes (multiple problems)  :: 6 of 67
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3129
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA114569841-rid20112183.denovo.148"
                     /isolate="VRE4027"
                     /db_xref="taxon:1352"
                     /country="Denmark"
     gene            103..1347
                     /locus_tag="SCJ86_002802"
                     /pseudo
     CDS             103..1347
                     /locus_tag="SCJ86_002802"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010729806.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="PRD domain-containing protein"
     gene            1360..1992
                     /locus_tag="SCJ86_002803"
     CDS             1360..1992
                     /locus_tag="SCJ86_002803"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010729807.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family phosphatase"
                     /protein_id="HEG1170285.1"
                     /translation="MTMGVIFDMDGVLIDSETFYFERRMNFFQELEIDPGSNQIDDYV
                     GKTEAAIWETLVPEDKSLREELYPKYVDYRKTHPIDFAKALRKEVKPLLDFLKKRDIP
                     LALASSSPAVEIESMLIQCELTEYFSFVISGESLKQSKPHPEIYLKAKQQLDCDFFIA
                     VEDSTIGIKSAKAANIYTAALKQPMQIDQSEADIQIESLQELEQIVLEKG"
     gene            2197..2424
                     /locus_tag="SCJ86_002804"
     CDS             2197..2424
                     /locus_tag="SCJ86_002804"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011837604.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ADP-ribosylglycohydrolase family protein"
                     /protein_id="HEG1170286.1"
                     /translation="MLQQNQNTNSYQAAILTAVNLGGDTDTTAAVTGALAGILYGGEV
                     IPENWRKSLQNDQLLKTISQRFCDTVLSQRI"
     gene            2493..2759
                     /locus_tag="SCJ86_002805"
     CDS             2493..2759
                     /locus_tag="SCJ86_002805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002383791.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antitoxin"
                     /protein_id="HEG1170287.1"
                     /translation="MLVQMMNGGRIMSQSTITFRIPDDEKELVLEYAKAHNSSLTELY
                     RNAVLDKIEDEIDLRTLRSAIEVSKEKKESGISQEEMEKLLNEL"
     gene            2749..3018
                     /locus_tag="SCJ86_002806"
     CDS             2749..3018
                     /locus_tag="SCJ86_002806"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334253.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="HEG1170288.1"
                     /translation="MNYKVIYMPQAQKQLKKMDRNQAKIIVAWIRKNLENTTDPRQHG
                     KGLTGNKSGEWRYRIGNYRILANICDSEIEIQIFSIGHRSTIYKR"
BASE COUNT         1102 a          466 c          617 g          944 t
ORIGIN      
        1 tgaaattgct ttcctttaaa atgactcatc gtcattcctc ctgctatctt tttctattat
       61 tctaccttat ctgatagtag atttaaaact ttgcaacaga accagaaaac caaagatcag
      121 cttaacgaat tttttgctaa tgttcttgaa accccttatt ggtatgcaaa aaaaatctta
      181 agccttttat ctgagtgttt agatcaacct gtgtatgaag aggacatttt ctttattacg
      241 ctatattttt atagtttgaa tgtcacgaat gttaaaacag gtattaagag tattgtgcta
      301 gctcatggtt attctacagc tagtagtatg gcgaatgttg ccaaccgtat gctgaataag
      361 aacgtttttc aagcttatga tatgccttta gatattacat tagagaaaat tgaagcaaaa
      421 ttgattcaat tccttggaga ttatcgaaca gattcaggct tgattttatt ggtagatatg
      481 ggttcactaa accaactagg cggtttatta gtgaatcatc taaatggacc actgttaatc
      541 attgatcata tcaatacacc gttattattg gaagtcggcc aacaagtatt aaacaataaa
      601 agtatcaccg aaataggagc agaaattgaa acaagtctta ctttcaaaaa gcagttacta
      661 cttcccaaga aaaagaaaaa gaaagccatc attacttgtt gttatacagg gattggtagt
      721 gcgacccaga ttcaggagat tttaacgaaa tgcttgaaag atgcagttag agaattagta
      781 attattccgt atgattacaa aaagctgtta gaaaacaaga attttgagac tccttttcaa
      841 ttatacgatg tcatcatgat tgtaggtaca gaagatccca aaattaatca gattccatat
      901 attggattag atcgcctgat taatggggga agcattggcg aatttgttca ttacttggaa
      961 aaagagctga ctattgaagt gaacgattta caaaaagaat taattttcaa tttttcaatt
     1021 agtaaaattg tcgaaaattt aaccattttg gatccagata aggtgctatc ttttgtacaa
     1081 aaagcaatta aagaattatc gaagttatta aattttgatt tttctaataa tcagctgttt
     1141 ttgttgtaca ttcactgtag ttgtatgatt gaacgaattg tacgtaaaga aagtgtggat
     1201 gagcaagagg acattgaaga atatatgcaa gcaaatggag aaataatgga taaaattcat
     1261 agcgcttttt ttgagattga aagggaatac acaattgaaa taccagattt agagttgcga
     1321 ttattgagcg atattgttca aggataggag tgaattcgaa tgacaatggg agtaattttt
     1381 gatatggatg gggtactcat agatagcgag acattttatt ttgagagaag aatgaacttc
     1441 tttcaagaac tggagataga tccaggatca aaccagattg atgattatgt ggggaaaaca
     1501 gaagctgcaa tatgggaaac attagttcct gaggacaaat cattaagaga ggaactttat
     1561 ccgaaatatg tagattatcg aaaaacgcat ccaattgatt tcgctaaagc tttacgaaaa
     1621 gaagtgaaac cgttacttga cttcctcaag aagcgggata taccattggc actggcatct
     1681 tcttctccag ctgtcgagat agagagtatg ctcattcaat gtgaacttac tgaatatttt
     1741 tcctttgtta taagtggtga gtcattaaaa caaagcaaac cacatcccga aatatattta
     1801 aaagcgaaac agcaattaga ctgtgacttc tttattgcag tagaggattc aacgattggt
     1861 attaaatcag caaaggcagc aaatatctat acggcggcac taaaacaacc aatgcaaatc
     1921 gatcagagtg aagcagacat acaaattgaa agtctacaag aactggaaca aattgtttta
     1981 gaaaaaggtt agtgaattaa tggaaataag gctatatatc tgtgctagct attttgacta
     2041 accaaaaaaa cttgctataa ttcaggtgta gtaaaaggtg ctggtaacac cttttacgaa
     2101 aatgcttcgg cagtgataat gggattggct ttttaagttt tttattttgt tcgcaatccc
     2161 taaggtcatg tgaatgatcc tagggattgc tagaatattt tgcaacagaa ccaaaatacg
     2221 aatagttatc aagcagccat attgactgcg gtcaatttag gaggggatac tgatactact
     2281 gctgccgtaa ctggtgctct tgcgggtatt ctttacgggg gtgaagtgat ccctgaaaat
     2341 tggagaaaat cgcttcaaaa tgaccagctt cttaaaacga tcagtcagag attttgcgat
     2401 accgtgctat ctcaaagaat ttgaataatg gatatgatgc aaatcttgtt tgtactaacg
     2461 ttgtattgac aaaaaggatt ttatttagta caatgttagt acaaatgatg aacggaggta
     2521 gaatcatgtc tcaatcaacc attacattta ggattcccga cgatgaaaaa gaactggtat
     2581 tagaatatgc aaaagcacac aattcttcat tgaccgaact ttatcgaaat gctgtactgg
     2641 ataaaatcga agatgaaatc gatctacgga ctttacggag tgcaattgaa gtttccaaag
     2701 agaaaaaaga gagtggtatt tctcaagagg aaatggagaa actgctaaat gaattataaa
     2761 gtcatctaca tgccgcaagc ccagaagcaa ttaaaaaaaa tggacagaaa ccaagctaaa
     2821 ataattgtcg cctggatcag aaagaattta gaaaacacga ctgacccgcg tcagcacgga
     2881 aaaggtttga ctggaaataa gagtggggaa tggcgctatc gaattggaaa ctatcgaata
     2941 ttggctaata tttgtgattc tgaaattgaa atccagatat tctcaattgg acatagaagt
     3001 acaatatata aaagataacc gtttttggaa cgaagtagag tttcgaaagt caatgtataa
     3061 tcttttgaat tttgcataat accacaggta gaaactaccc gatactatcg gttggtttct
     3121 gctttttta
//
LOCUS       DARBET010000143         3018 bp    DNA     linear   BCT 07-NOV-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4027
            SAMEA114569841-rid20112183.denovo.149, whole genome shotgun
            sequence.
ACCESSION   DARBET010000143 DARBET010000000
VERSION     DARBET010000143.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA114569841
            Sequence Read Archive: ERR12203666
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3018)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3018)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001972618.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001972618.1
            Genome Coverage       :: 132x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/07/2023 13:18:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,041
            CDSs (total)                      :: 2,975
            Genes (coding)                    :: 2,908
            CDSs (with protein)               :: 2,908
            Genes (RNA)                       :: 66
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 67
            CDSs (without protein)            :: 67
            Pseudo Genes (ambiguous residues) :: 0 of 67
            Pseudo Genes (frameshifted)       :: 26 of 67
            Pseudo Genes (incomplete)         :: 28 of 67
            Pseudo Genes (internal stop)      :: 20 of 67
            Pseudo Genes (multiple problems)  :: 6 of 67
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3018
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA114569841-rid20112183.denovo.149"
                     /isolate="VRE4027"
                     /db_xref="taxon:1352"
                     /country="Denmark"
     gene            201..680
                     /locus_tag="SCJ86_002807"
     CDS             201..680
                     /locus_tag="SCJ86_002807"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400217.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEG1170289.1"
                     /translation="MEIPTNLKHKPVIVVEDYDKVDGRNALNMDAKGLSLGLAQWNDR
                     GKVDISGKVWRHTGEKWSRQSEELPITRILDLAILAAQGSLYFQDAYRYEKFYDPENP
                     VVDIIGLQGNRMTVEVDTKNPKIDEDIILYYDTLQKDGELIGERFRILKRLLDELGY"
     gene            713..2941
                     /locus_tag="SCJ86_002808"
     CDS             713..2941
                     /locus_tag="SCJ86_002808"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HEG1170290.1"
                     /translation="MENFSLTENRTFSRLEKQMIWKKPKSHRTSAEEWRIASEIKGNW
                     QDPEMKISNVLLEGDAGSGKTQLAKALSADLQLPYTKVTCFADMDKSDVFGALLPVVD
                     SNKEQDQELLEAIYQTDTLEAVLTLIQNHYGIDPFSAKEKLAELVQRIEADYPDAIHY
                     SYYPSEIVRAIEKGYLLEIQEPTVIRDASVLVALNSALEPNGMLNLPTGIIRRHPDCV
                     IVITTNRNYQGNRPLNESLRDRMQHAEKMDLPALEVMAERAMAKTMIQKQELLLKMAE
                     IIRLLDETAKANAIKGVAGMRSYFYWANTYKQGQDLLQSIYPKVLYKLSTDSDELAIL
                     EQALSDSGLLLELQDILRKEKWGHPQSEHTKERTISAEEANERNITENAEEILRLDEK
                     KSLSAEEPNEGEEKQEETIEKNEIPPQMQKERSSDIEGKNQKEQQTGDDSQEMSASDM
                     EAHDKEIKKQLNKEARQIMKETIHEKEGLIVHRPAFQGKTPEARELHMQVMPIVESLS
                     RQILELLDNEQTETYQKGKYEGQRFNASKVAYGDLRNFDKKNPPHEQPSLAVAVRIDE
                     SGSMIRDDRIEAAKKAAIAIAEFSKKVDIPLLIYGDTADRSSREKTSLFSYKEFEDGF
                     QWLNEKLVTMKPRQNNRDGAALRLAAEKLNRQSATTKLLLNISDGQPKALPDYTGKKA
                     KEDIQSVLKEYERQGILFVSAAIGQDKEEIKSIYGESRFVDITDLNEFPKQMIQLIAR
                     YL"
BASE COUNT         1101 a          544 c          627 g          746 t
ORIGIN      
        1 attccaacat catcttggac tttctacatg ttactaatga gcgtgtaaaa gaacgagatg
       61 gtgtttttat ttctatcaat gaagctcttt ttctttgcaa tcctcacttc aatcctctat
      121 aatgtcgcat atagtttagt aaatgagtaa actactaatt tacttattta ctaaaaataa
      181 aaggatgagg agttaaaata atggaaatac caacaaattt aaaacataaa ccagtaatcg
      241 ttgtagaaga ctatgataaa gttgatggaa gaaatgcatt aaatatggat gcaaaagggc
      301 tttcactggg ccttgctcag tggaatgatc gtggaaaagt cgatatctca ggcaaggtat
      361 ggcgacatac tggagaaaaa tggtcacgac agtcggaaga attaccaatc acacgtattt
      421 tagatttagc gatcttagct gcgcaaggaa gtttatactt ccaagatgct tatcgttatg
      481 aaaaatttta tgatccagaa aatccagtcg tagacatcat tggattacaa gggaaccgaa
      541 tgacggtaga agtagatacc aaaaatccaa agatcgatga agacatcatt ttgtattacg
      601 atacactaca aaaagacgga gaactgattg gggaacgatt ccgtatttta aaacgtcttt
      661 tagatgagct aggatattaa tagacagaga tgatcaaaag gggaagagaa gcatggagaa
      721 tttctcacta acagaaaacc gaaccttttc acggctagaa aagcaaatga tctggaagaa
      781 accgaaaagc catcggacaa gtgcagaaga atggcggatc gcctccgaaa tcaaagggaa
      841 ttggcaagat cctgaaatga aaatcagtaa cgtacttttg gaaggagatg ccgggtcggg
      901 gaagacacaa ttagccaaag cactatctgc tgatctgcaa cttccctata caaaagtgac
      961 ctgttttgca gatatggaca aatcagatgt tttcggagcc ttattacctg tagtagattc
     1021 taataaggaa caagaccaag aattactaga agctatttac caaacagata cacttgaagc
     1081 tgtactgaca ctgatccaga accattacgg cattgatcct ttttctgcta aagaaaaatt
     1141 agcagagcta gttcaaagga tcgaagctga ttatccagat gcaatccatt acagctatta
     1201 cccttctgaa atcgttcgag cgattgaaaa aggctattta ttagagattc aagagccgac
     1261 tgtcatccga gatgcctctg ttcttgtggc acttaactca gcgctcgaac caaatgggat
     1321 gttgaattta ccgacaggaa tcattcgtcg ccatccagat tgcgtcattg tgatcactac
     1381 aaatcgaaat taccaaggaa atcgcccatt aaatgaatct ttaagagatc ggatgcaaca
     1441 tgcggaaaaa atggatcttc ctgccttaga agtcatggca gaacgtgcga tggcgaaaac
     1501 tatgatccaa aaacaagaac ttctcttaaa gatggcagag attatccgtt tgttggatga
     1561 aactgcaaaa gctaacgcca tcaaaggtgt agcaggaatg cgttcttatt tttattgggc
     1621 aaatacctat aagcaaggac aagatttact ccagtcgatt tatccaaaag ttttgtataa
     1681 attatccact gattcagatg aattggcaat tcttgaacaa gctttgtctg acagtggatt
     1741 gctactggaa cttcaggaca tattgagaaa agaaaaatgg ggtcatcctc aatctgagca
     1801 tacaaaagaa agaaccatta gcgccgaaga agcaaatgaa cgaaacatca ctgaaaatgc
     1861 tgaagaaatt ctgcgcttag atgaaaagaa atcgttgtct gctgaagaac caaatgaggg
     1921 agaagagaaa caagaagaaa caatagaaaa aaacgagatt cctcctcaaa tgcaaaaaga
     1981 acgcagtagc gatatcgaag gaaaaaatca aaaagaacaa caaacaggag atgattctca
     2041 agaaatgtct gcttctgaca tggaagcaca tgataaggaa atcaagaagc aactgaataa
     2101 agaagcgcga caaatcatga aggaaacgat tcatgaaaaa gaaggtctca tcgttcaccg
     2161 accagctttc caaggaaaaa cgccggaagc acgtgagcta cacatgcaag taatgccaat
     2221 agtagaatca cttagccggc aaattttaga gttactggat aatgaacaaa cagagacgta
     2281 ccaaaaagga aaatacgaag gtcagcggtt caatgcaagc aaagtagcat atggtgattt
     2341 gagaaatttt gacaaaaaga acccgccaca tgagcaacct tctttagcgg tagctgtacg
     2401 gatcgatgaa tctgggtcaa tgatacggga tgatcggatc gaagcggcaa aaaaagcagc
     2461 gattgccatt gccgaatttt ctaagaaagt cgatatccca ttgttgattt acggagatac
     2521 agcagaccgt tcgtcaagag aaaagacttc tttgttttct tataaagaat ttgaagacgg
     2581 atttcaatgg ttaaatgaaa aattagtcac tatgaaacca aggcaaaaca atcgtgatgg
     2641 tgctgcactt cgtttagcag cagaaaaatt aaacagacaa tccgcaacaa ccaaactgtt
     2701 gcttaatatt agtgacggtc agccaaaagc attgccggat tatacaggca aaaaagcaaa
     2761 agaagatatc cagtctgttt tgaaagaata tgaacgtcaa ggaatcctat ttgtctccgc
     2821 agctattggc caagacaaag aagaaatcaa gtcgatctac ggtgaaagtc gttttgtaga
     2881 tattactgac ttgaatgaat ttcccaagca aatgattcaa ctgattgccc gctatctatg
     2941 ataaaataaa gctcaacgaa cgcacttagc agattcgttg agcttttcgt ttctagttac
     3001 cggtaacaag gatacttc
//
LOCUS       DARBET010000144         2927 bp    DNA     linear   BCT 07-NOV-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4027
            SAMEA114569841-rid20112183.denovo.150, whole genome shotgun
            sequence.
ACCESSION   DARBET010000144 DARBET010000000
VERSION     DARBET010000144.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA114569841
            Sequence Read Archive: ERR12203666
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2927)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2927)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001972618.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001972618.1
            Genome Coverage       :: 132x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/07/2023 13:18:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,041
            CDSs (total)                      :: 2,975
            Genes (coding)                    :: 2,908
            CDSs (with protein)               :: 2,908
            Genes (RNA)                       :: 66
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 67
            CDSs (without protein)            :: 67
            Pseudo Genes (ambiguous residues) :: 0 of 67
            Pseudo Genes (frameshifted)       :: 26 of 67
            Pseudo Genes (incomplete)         :: 28 of 67
            Pseudo Genes (internal stop)      :: 20 of 67
            Pseudo Genes (multiple problems)  :: 6 of 67
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2927
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA114569841-rid20112183.denovo.150"
                     /isolate="VRE4027"
                     /db_xref="taxon:1352"
                     /country="Denmark"
     gene            complement(45..1364)
                     /locus_tag="SCJ86_002809"
     CDS             complement(45..1364)
                     /locus_tag="SCJ86_002809"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008392738.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing histidine kinase"
                     /protein_id="HEG1170291.1"
                     /translation="MKKLKIFPKMFIQIFSVLGIIIILVHSLIFFIFPKTYLETRKEK
                     IHNMANEISSNMNGKEIKYIEQTLEFYSKSSEIKVFIKEKNNKNEIQIKDNINVNLES
                     DSNSLIIEEREIKLNDGKKTNLQFVSTADMQKDAKDLSLKFLPYSLLISILFSAIISL
                     IYAKSIKNNIQEIKIVTDKMMKLDKKMSLKVSSNDEVGELKQQINDLYSTLLRTIDDL
                     EFKNKEILKLEKLKYDFFKGASHELKTPLASLKIILENMKYNIGKYKERDIYINECIE
                     IVDSLTKNISQILSVHSIENLNNDEEYLKINDTLEDILKKYEILANQKKITVNNYILD
                     ENVYIGKTALKIILSNLISNAVKYTDVNGVINIGIVNDWLYIENSYGNNKISNMDKIF
                     DVKFDLNKENSNGLGLYIVSNILNNYNIEYKVLQDEEFFIFKIKIFS"
     gene            complement(1361..2014)
                     /locus_tag="SCJ86_002810"
     CDS             complement(1361..2014)
                     /locus_tag="SCJ86_002810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334003.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HEG1170292.1"
                     /translation="MKILTVEDDNMIREGISEYLSEFGYTVIQAKDGREALSKFNSDI
                     NLVILDIQIPFINGLEVLKEIRKKSNLPILILTAFSDEEYKIDAFTNLVDGYVEKPFS
                     LPVLKARIDSLIKKNYGHLEKFEYKDLSVNFNSYTAKINGEEIDVNAKELEVLKCLLD
                     NNGQVLTRMQIIDYVWKDSEETPYDRVIDVYIKELRKKLQLDCITTIRNVGYKLERK"
     gene            2302..>2927
                     /locus_tag="SCJ86_002811"
     CDS             2302..>2927
                     /locus_tag="SCJ86_002811"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HEG1170293.1"
                     /translation="MDNHTRKLLGLTDKHLFFDEEWLIEKEYKGVQAQFIKGRLDDSP
                     SHCEHCGSIRIIRNGSYTTRTQILKVKEKLTILELKRTRFLCYDCGQTFSAKTDLVDE
                     HHQLTKELKQAILIELYENQSRKLIAKKYFVSDGTVTRILREATKHYQPRMNFLPTVL
                     CMDEFKSMKSVSGSMSFICVDGTTHQLFTILEDRRLYKLTQYFLRFPR"
BASE COUNT          959 a          477 c          323 g         1168 t
ORIGIN      
        1 ataagtctct atttttcaat aactattgca aaattatatc ttaattaaga aaatattttt
       61 attttaaaga taaaaaattc ttcatcctgc aatactttat attctatatt gtaattattc
      121 agaatattac ttacaatata taaacctaat ccattactat tttccttatt taaatcaaat
      181 ttaacatcaa atattttatc catattcgaa attttattgt taccgtatga attctctata
      241 tataaccaat cattaactat cccaatatta attaccccat ttacatcagt atattttacc
      301 gcattactaa tcaaattaga tagaataatc tttaaagctg tttttcctat ataaacattc
      361 tcgtctaata tataattatt gacagttatt ttcttttgat ttgctaatat ttcatacttt
      421 tttaatatgt cttctaatgt atcatttatt ttcaaatatt cttcatcatt attcaaattt
      481 tctatagaat gaactgataa tatttgagaa atattttttg ttaaactatc aacgatttca
      541 atacattcat taatataaat atctctttct ttatattttc caatattgta tttcatgttt
      601 tccagtatta ttttaagact agcaagaggg gttttaagtt catgggatgc acctttaaaa
      661 aagtcatatt ttaacttttc taattttaaa atttctttat ttttaaattc caaatcgtca
      721 attgttctta ataaagtaga atacaaatca ttaatttgtt gttttaattc tccaacttca
      781 tcattagagc taacttttaa actcattttt ttatcaagtt tcatcatttt atcagtaaca
      841 atttttattt cttgtatatt attttttatt gattttgcat aaattaaaga aataatagca
      901 gaaaataaaa ttgatattaa taaagaatat ggtaaaaatt taaggcttaa atctttcgca
      961 tctttttgca tatcagctgt agaaacaaat tgtagatttg tttttttacc atcattaagc
     1021 tttatttctc tttcttcaat tatcaaagaa ttactatcac tttctaaatt aacatttata
     1081 ttatctttga tttgtatttc attcttatta tttttttctt ttatgaatac ttttatttca
     1141 ctactcttgg aataaaactc caaagtttgt tctatatatt ttatttcttt tccattcata
     1201 ttactagaaa tttcatttgc catattatga atcttttctt ttctcgtctc caaatatgtt
     1261 ttaggaaaga taaaaaaaat taatgaatga actaatataa ttattattcc aagaacagaa
     1321 aatatttgta tgaacatttt tggaaatatc tttaattttt tcattttctc tccaatttat
     1381 atcctacatt tcttatagta gttatacagt ctaattgtaa tttttttcta agttctttta
     1441 tatatacatc tattactcga tcataaggag tttcttcgct atctttccat acataatcaa
     1501 taatttgcat tcttgttaac acttgtccat tattatccaa taaacatttt aatacttcta
     1561 gttcttttgc atttacatct atttcttcac catttatttt agctgtatag ctattaaaat
     1621 taaccgatag atccttatat tcaaacttct ctaaatgtcc ataattcttc ttaattaaag
     1681 aatctattct agcttttaag accggcaatg aaaatggctt ttctacatat ccgtctacta
     1741 aattagtaaa tgcatcaatt ttatattctt catcactaaa tgcagtcaag attagaattg
     1801 gtaaattgct tttctttcta atttctttca acacttctaa accatttata aaaggtatct
     1861 gaatgtctaa gataaccaaa tttatatcgc tattaaattt tgacaaggct tctcttccat
     1921 ctttagcttg aataacagta tatccaaatt ctgaaagata ttcacttatt ccctctctta
     1981 tcatattatc atcttcaaca gttaatattt tcatatatac ctccacttaa gcctatacac
     2041 tagatattat acagcatatt atggctctat aatatctagt gttggtgaca ccttctgttg
     2101 ccaatacttt ttcttttttc taataaatgc aaacagacac cttgaaatcc tttagaataa
     2161 agggttctat aatattttgt gttgttggaa aatcgaaggc gattttctac taacactttt
     2221 tcttcgtttc aactcaaaaa gagactcact tttggtaaaa tttagtcgac caaaacatca
     2281 ccctaccaaa ggagtcgctc tatggataat catactagaa aattactcgg attaacagat
     2341 aaacatttat tttttgatga agaatggctg attgaaaaag aatataaagg cgttcaagcg
     2401 cagtttatta aagggagatt ggatgactca ccttcccatt gtgaacactg tggatcgatt
     2461 cgtattatcc gcaacggttc ctatacgaca cgtactcaga tattgaaagt caaagaaaag
     2521 ttgactattt tagaattaaa acgtacccgt tttctatgct atgattgtgg gcagaccttt
     2581 tctgcgaaaa cggacttagt tgatgaacat catcaactaa caaaggaact gaagcaggcg
     2641 attctcatag aactgtacga aaatcaatca cgtaaactaa tcgctaagaa gtacttcgtc
     2701 tcggatggga cggtgacacg gatcttgcgt gaagcaacga agcattacca gccacgtatg
     2761 aacttccttc cgacggtgtt gtgtatggat gagttcaaat caatgaagtc tgtttctggt
     2821 tccatgagtt ttatttgtgt tgatgggaca acccatcagc tatttacgat ccttgaagat
     2881 cgcagacttt acaaactaac tcaatacttc ctgcgctttc ctagaaa
//
LOCUS       DARBET010000145         2867 bp    DNA     linear   BCT 07-NOV-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4027
            SAMEA114569841-rid20112183.denovo.151, whole genome shotgun
            sequence.
ACCESSION   DARBET010000145 DARBET010000000
VERSION     DARBET010000145.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA114569841
            Sequence Read Archive: ERR12203666
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2867)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2867)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001972618.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001972618.1
            Genome Coverage       :: 132x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/07/2023 13:18:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,041
            CDSs (total)                      :: 2,975
            Genes (coding)                    :: 2,908
            CDSs (with protein)               :: 2,908
            Genes (RNA)                       :: 66
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 67
            CDSs (without protein)            :: 67
            Pseudo Genes (ambiguous residues) :: 0 of 67
            Pseudo Genes (frameshifted)       :: 26 of 67
            Pseudo Genes (incomplete)         :: 28 of 67
            Pseudo Genes (internal stop)      :: 20 of 67
            Pseudo Genes (multiple problems)  :: 6 of 67
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2867
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA114569841-rid20112183.denovo.151"
                     /isolate="VRE4027"
                     /db_xref="taxon:1352"
                     /country="Denmark"
     gene            <1..1269
                     /locus_tag="SCJ86_002812"
     CDS             <1..1269
                     /locus_tag="SCJ86_002812"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016250137.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LPXTG cell wall anchor domain-containing
                     protein"
                     /protein_id="HEG1170294.1"
                     /translation="NYEGVIEVTYPDGTKDTVKVPVEVTDNRSDADKYEPTVEGEKVE
                     IGGTVDLTDNVTNLPALPEGTTVTDVTPGGTIDTNTPGNYEGVIEVTYPDGTKDTVKV
                     PVEVTDNRSNADKYNPEGQKVTTDLNKEPDASEGIKNKEDLPKGTTYAWKEKVDVSTA
                     GNKKGTIVVTYPDGSSDEVEVDVTVTDTRSDADKYNPEGQKVTTDLNKEPDASEGIKN
                     KEDLPKGTTYTWKEKVDVSTAGNKKGTVVVTYPDGSKEEVEVTISVVDKKAPNKPQVD
                     PITEGDQIVTGKTEPNAEVTVTLPDGSQHHGTADKNGNFTVKVPKLEAGTKVIVTATD
                     ESGNTSEPTNVVVSSNKKDSGKNGSKGSKTDNQGSNSNQDKNRGKSQSSKVFPKTGES
                     DSNIFTISGGLILLGTLGLLGYKNRKKENE"
     gene            1403..2746
                     /locus_tag="SCJ86_002813"
     CDS             1403..2746
                     /locus_tag="SCJ86_002813"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HEG1170295.1"
                     /translation="MEKIVIIGSNHSGLAAARFLNLSDSNYEVILIDKKSEISYLECG
                     TSLFIKGEVSNLEDLFYQNRMTLNSEIKKLFLETEVDKIDIQNKKISCVKKDGTRFSQ
                     EYDKVILATGSKQIMLDIPGNNLANIFSIKNFPNAKEIFKALDDSTIKKVGIIGAGYI
                     GVEIAEAIRKRGKEVYLFDVADRVLSTYYDRSFSDKVEEILSKNGIHLCLSESPKKYL
                     GRKKIEKIVTTSGTQYSMDMVVQAIGFLSNSPIGEKELLRDVSGAYLTNEYQQTNIKD
                     IYAIGDCATTFFTSINRSSVDFSISNALRTAYIATQHINNQDFTPSGSQFTNGFSIFN
                     YNFYAAGLNLKTARLLSESIDYIEIEDFIRPAFLRKNSRVKLRISFEKNTKRIVGVQL
                     CSQEDLSGLLSMFSLAIEEQITLDKLKMLDYLFHPSFSQPYNFILKAVTGLNIET"
BASE COUNT         1143 a          376 c          545 g          803 t
ORIGIN      
        1 aattacgaag gtgtcattga agtaacgtat ccagacggta cgaaagatac ggtgaaagtt
       61 ccagtagaag tgacagacaa tcgttctgac gccgataaat atgaaccaac agtagaaggc
      121 gaaaaagtag aaattggagg cacagtagat ttaacagata atgttactaa cttgccagcg
      181 ttaccagaag gaacaacagt aacagatgtt actcccggcg gtacaattga taccaacaca
      241 ccaggcaatt atgaaggtgt cattgaagta acgtatccag acggtacgaa agatacggtg
      301 aaagttccag tagaagtgac agacaatcgt tctaatgcag ataaatacaa tcctgaagga
      361 caaaaagtga ctacagactt aaataaagag ccagatgcat ctgagggaat taagaacaaa
      421 gaagatttac caaaaggaac tacttatgct tggaaagaga aagtagatgt tagcacagct
      481 ggaaataaaa aaggtactat tgtagtgaca tatccagatg gatcatctga tgaagttgaa
      541 gtagacgtta cagtgacaga tactcgttcc gatgcagata aatataatcc tgaaggacaa
      601 aaagtgacta cagacttaaa taaagagcca gatgcatctg agggaattaa gaacaaagaa
      661 gatctaccaa aaggaactac ttatacttgg aaagaaaaag tagatgttag tacagctgga
      721 aacaaaaaag gtactgtagt agtaacatat ccagatggat ctaaagaaga agtagaagtt
      781 actatttctg tagtagataa aaaagcaccg aataaacctc aagttgatcc tattacagag
      841 ggtgaccaaa ttgttactgg taaaactgaa ccaaatgcag aggtgacagt tacattacca
      901 gacggaagtc aacaccatgg gacagctgat aaaaatggta attttacagt taaagttcct
      961 aaattagagg cagggacaaa agtgatagta actgcaactg atgaatctgg taatactagt
     1021 gaacctacta atgtagttgt ttcttccaac aaaaaagaca gtggaaaaaa cggtagtaaa
     1081 ggtagtaaga ctgacaatca aggtagcaac agcaatcaag acaaaaatag aggcaaaagc
     1141 cagtcctcaa aagtttttcc taagacggga gagagtgaca gtaatatctt tactattagc
     1201 ggtggtttaa tactgttagg aacgttagga ttattagggt ataaaaatcg aaaaaaagag
     1261 aatgaataga aaatcaattt cttaagtctt ctctgagaag aggaagaatc tttttcttcc
     1321 tctttttgct tttttatgat gggaaataag tgttagttaa agatgaatat actttttata
     1381 gaaaggaaaa gaatgtaatt aagtggaaaa aatagtaatt attggttcaa accattcagg
     1441 gctagccgca gctcgatttc taaacttaag cgattcaaat tatgaagtta ttctaatcga
     1501 taaaaaatca gaaattagtt acttggaatg tgggacatcg ttatttataa aaggagaagt
     1561 aagtaattta gaagatcttt tttatcaaaa tagaatgaca ctaaattctg agataaaaaa
     1621 gttatttcta gagacagaag ttgacaaaat tgatattcaa aataaaaaga ttagttgtgt
     1681 aaaaaaagat ggtacaagat tctctcaaga atatgacaaa gttatattag ctacaggttc
     1741 aaagcaaatt atgctagata taccggggaa taatttagct aatatttttt cgataaagaa
     1801 ttttccaaat gcaaaagaga tttttaaagc tttggatgat agtactatta aaaaagtagg
     1861 aattattgga gcaggatata ttggagttga aattgctgaa gccataagaa aaagagggaa
     1921 agaagtatat cttttcgatg ttgcagatag agtgttgtcg acttattacg accgtagttt
     1981 ttcagataaa gttgaagaaa tactatcaaa aaatggaata catctttgtc tatcagaaag
     2041 tcctaaaaaa tatttaggaa gaaaaaagat tgaaaaaata gtcactacaa gtggtaccca
     2101 atactctatg gatatggtcg tacaagctat aggatttcta tctaatagtc caattgggga
     2161 aaaggaactc ttgagagatg ttagtggagc atatctaact aatgaatacc agcaaacaaa
     2221 tataaaagat atatatgcta ttggggattg tgctacaact ttttttacgt ccattaatag
     2281 aagtagtgtt gatttttcta tttctaatgc tctaagaaca gcttatattg caactcaaca
     2341 tattaacaat caagatttta caccttctgg ttcccaattt acaaatggct tttctatatt
     2401 taattacaat ttttacgcag caggtttgaa tttaaaaaca gcacgattat taagtgagtc
     2461 tatagattat atagaaatag aagatttcat tagacctgct tttttgagaa aaaatagtcg
     2521 tgtaaaatta agaattagct ttgaaaaaaa tacaaagaga atagtaggtg tacaactttg
     2581 tagtcaagaa gatttatctg gactattatc aatgttttct cttgctatag aagaacaaat
     2641 tacattagac aaattgaaaa tgttagatta tttatttcat cctagtttta gccaacccta
     2701 taattttatt ttaaaggcag taacaggatt aaacattgaa acataatcgt tactaaatga
     2761 aatcaagaaa tatactgtat tactcaattc attgaaaata tttatcataa tttttaatag
     2821 aaaacactat taaagagaaa aaagagaggt aaatcaaatg aaaaaat
//
LOCUS       DARBET010000146         2844 bp    DNA     linear   BCT 07-NOV-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4027
            SAMEA114569841-rid20112183.denovo.152, whole genome shotgun
            sequence.
ACCESSION   DARBET010000146 DARBET010000000
VERSION     DARBET010000146.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA114569841
            Sequence Read Archive: ERR12203666
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2844)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2844)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001972618.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001972618.1
            Genome Coverage       :: 132x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/07/2023 13:18:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,041
            CDSs (total)                      :: 2,975
            Genes (coding)                    :: 2,908
            CDSs (with protein)               :: 2,908
            Genes (RNA)                       :: 66
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 67
            CDSs (without protein)            :: 67
            Pseudo Genes (ambiguous residues) :: 0 of 67
            Pseudo Genes (frameshifted)       :: 26 of 67
            Pseudo Genes (incomplete)         :: 28 of 67
            Pseudo Genes (internal stop)      :: 20 of 67
            Pseudo Genes (multiple problems)  :: 6 of 67
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2844
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA114569841-rid20112183.denovo.152"
                     /isolate="VRE4027"
                     /db_xref="taxon:1352"
                     /country="Denmark"
     gene            144..494
                     /locus_tag="SCJ86_002814"
     CDS             144..494
                     /locus_tag="SCJ86_002814"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324485.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEG1170296.1"
                     /translation="MSRLIIQTAFYKAIIPENLDEVKLYLWEDNGEIVGFSGVSAEEL
                     VMLFLDPDFIGKRYGSQILIELIETEDIKRIDVNSQNKYAKHFYLSHGFEIESEEDLD
                     GFGKPYPITHLVKK"
     gene            908..1726
                     /locus_tag="SCJ86_002815"
     CDS             908..1726
                     /locus_tag="SCJ86_002815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341332.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HEG1170297.1"
                     /translation="MNKNPHNLKLVAVDMDGTFARTDYTYDIPRFKAVLSRMKAVDCQ
                     FVVASGNQYYQLRSLFPNYYDELSFVAENGAFVKDKTEVLFSADMPRKAIEVVLDLCK
                     DYPEVKNVMCGLNSAYCERGKVSQAFFDLTNIYYHRLEWVADFAEVQDKILKFAPTMP
                     VEKTEFYVELFREKLAGLLVPTSSGHGSVDLILSECHKTFGLKMLVERLGINPDQCVA
                     FGDGGNDIEMLEYCGLSYEMDNATEAVKQVAKHQCPSNDEDGVLVTLDRLFPNT"
     gene            complement(1799..2158)
                     /locus_tag="SCJ86_002816"
     CDS             complement(1799..2158)
                     /locus_tag="SCJ86_002816"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-sulfur cluster biosynthesis family protein"
                     /protein_id="HEG1170298.1"
                     /translation="MSDNLFLEVQPEVFKQVPRLKDGFLLTLNDGSNQFSSAEGCCMI
                     GDHFLLVSTQHYIPAYDLTIPCEEATVKTSEYDTTFINGHLVLEVTPSNQTIRLKNES
                     GILDSNVQVYFDSSDAS"
BASE COUNT          969 a          399 c          505 g          971 t
ORIGIN      
        1 gcaaccggag tttcttgatg aaaatatgct tctatcatta caagctcgtt tggtgtaaga
       61 tgggtataag tcatttatgt tcactctcct tgtatgcttt agcgggtatt acaatttgag
      121 tgtaacataa atggcttttt tatttgtctc gcttaattat acaaacggcg ttttataaag
      181 caatcattcc tgagaactta gatgaggtta aactttattt atgggaagat aacggagaaa
      241 ttgtcggttt tagtggtgta agtgcagaag aattagttat gctatttctt gatccagatt
      301 ttattggtaa gcgatatggt agtcaaatat taattgagct gattgaaact gaagatataa
      361 aaagaattga tgttaatagt caaaataaat atgcaaaaca tttttattta agccatggat
      421 ttgaaattga atctgaagaa gacttagatg gttttggtaa accataccct ataacgcatt
      481 tagtaaaaaa ataattcatg aatggtttat tacctgattg aacatgaata aaagtaatat
      541 ttttattttg atggcctatt tctaggttat gaattatcca gatcaaattt gtgtattaca
      601 ttcagtaata aggtgcttat cttaggatca acttatgata ctagctgacc aaggatgtac
      661 cagttataga aaatcagtta aagtaaaaat gataaaaatc ttatgtcaac actgatttag
      721 gctgacctgt gggttaggag aaagacaggt cggtagcttt taatacaaaa aatggcttgt
      781 atgtatctag atagttaact gaaattagaa atgttcttat ttttaacgga gtaagaaaaa
      841 taggctgctt taggtctgaa tactttttca atataatgaa gatatcaaag gcgacaggag
      901 tgtttttatg aataagaatc ctcataatct aaagttggta gcagtagata tggatggaac
      961 atttgctcgt actgattaca catacgatat cccaaggttc aaggctgttt tatcgcgaat
     1021 gaaagctgtt gattgtcaat ttgttgttgc aagtggaaat caatactacc aattgagatc
     1081 gttatttcca aattattatg atgaattaag ttttgttgct gaaaatggtg cttttgttaa
     1141 agataaaaca gaggtactat tttcagccga tatgcctaga aaagcgatcg aggtggtcct
     1201 tgatttatgt aaagactatc ctgaagtaaa aaatgtgatg tgtggtctga atagtgccta
     1261 ctgtgaaaga ggaaaggtga gtcaagcatt ttttgatcta accaatatct attaccatag
     1321 attagaatgg gtagctgatt ttgcagaagt tcaagataaa attttaaaat ttgctccgac
     1381 tatgcctgtt gaaaaaaccg aattttacgt tgaattgttc cgtgaaaaat tagccgggct
     1441 attagtacct accagtagtg gtcatggttc tgtagacttg attctttctg agtgccataa
     1501 aactttcggc ttgaaaatgt tagttgagcg cttggggatt aatcctgacc agtgcgttgc
     1561 ttttggagat gggggcaatg acattgagat gttggagtat tgtggtctga gttatgaaat
     1621 ggataatgca acggaagctg ttaagcaagt tgctaagcat caatgtcctt caaatgacga
     1681 agacggtgtt ttagtgacgc tcgatcgatt gttccctaac acgtaaacac taggtaattc
     1741 atcaaaaaac ctcgatataa gaaatattcg aggttttaaa actatattta gaacagattt
     1801 acgaagcgtc tgatgagtca aaatagactt ggacattgct atctaaaata ccactttcat
     1861 tctttaaacg aattgtttga tttgatggag ttacctctaa tactaaatgc ccattaataa
     1921 aggtagtatc gtattcagat gttttaactg ttgcttcttc gcaaggtatt gttaaatcat
     1981 atgctggaat ataatgttgt gtggaaacta ataaaaaatg atcaccaatc atacagcatc
     2041 cctctgctga agaaaattga ttagagccat catttaaagt taacaagaat ccatccttta
     2101 aacgaggtac ttgcttaaat acttctggtt gtacttctaa aaataaatta tcgctcattt
     2161 tcacgaccac catcgacttc cacagttgaa attgacattt tatttaacac acctattccc
     2221 ccttaactat aactcaattc tatataaaaa atattctatt ctcaaataat tgaacttttc
     2281 aaatcgccaa tttacttcgg ctttatcaga gctcattcat atcaagagcg attaacgctg
     2341 taaaaattaa accgaagatc tgataaattt tctctatttt ctacaatatt taaaacgctt
     2401 ccttagtcta ttatatatct atttctctta ggagtatgtt tagatgtttg aataatagtt
     2461 gcccaacttt aaaggttatt cttataatat tttacttcat tttccaatag aaaattcaaa
     2521 ggattactgg tttttctaaa atagtttagt ttaaatacat aagtatcctc cgacactgta
     2581 ttagttcata atcatctaaa tacaaaattt tcaaataaaa atatttcaag ctctatcgtt
     2641 gacgaatgta aacgatactt ataaagtaat agaaattgat tacatttgta aacttaaaat
     2701 ggttaattta tctcacataa aactaagaat aaaataatga ttaaaagtgg ttagttttta
     2761 tacgatttag cctatcggta aaaatgaatt agagagtgta aaatattttg tgtaaatgaa
     2821 aaaatccata caaaaaagga agtc
//
LOCUS       DARBET010000147         2816 bp    DNA     linear   BCT 07-NOV-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4027
            SAMEA114569841-rid20112183.denovo.153, whole genome shotgun
            sequence.
ACCESSION   DARBET010000147 DARBET010000000
VERSION     DARBET010000147.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA114569841
            Sequence Read Archive: ERR12203666
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2816)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2816)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001972618.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001972618.1
            Genome Coverage       :: 132x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/07/2023 13:18:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,041
            CDSs (total)                      :: 2,975
            Genes (coding)                    :: 2,908
            CDSs (with protein)               :: 2,908
            Genes (RNA)                       :: 66
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 67
            CDSs (without protein)            :: 67
            Pseudo Genes (ambiguous residues) :: 0 of 67
            Pseudo Genes (frameshifted)       :: 26 of 67
            Pseudo Genes (incomplete)         :: 28 of 67
            Pseudo Genes (internal stop)      :: 20 of 67
            Pseudo Genes (multiple problems)  :: 6 of 67
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2816
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA114569841-rid20112183.denovo.153"
                     /isolate="VRE4027"
                     /db_xref="taxon:1352"
                     /country="Denmark"
     gene            62..247
                     /locus_tag="SCJ86_002817"
     CDS             62..247
                     /locus_tag="SCJ86_002817"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317758.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HEG1170299.1"
                     /translation="MSENLDLKIRAEMRKRRMTFKELAALIGISGAYLSDILNGNRDG
                     KKAQQHIETVKKILDIR"
     gene            259..507
                     /locus_tag="SCJ86_002818"
     CDS             259..507
                     /locus_tag="SCJ86_002818"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341599.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEG1170300.1"
                     /translation="MTKLKKQDFVKKYNYSPSTYQRRMSELKNTAIFSAAYERVTGQE
                     VWINTELYDKFLSFKSYNRLRTRKVTPKEFIEKHLVDL"
     gene            538..717
                     /locus_tag="SCJ86_002819"
     CDS             538..717
                     /locus_tag="SCJ86_002819"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296610.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEG1170301.1"
                     /translation="MGKFNRALVFSAPLIIYALGLWGSRQALIGTIVYMVWIFMGLDE
                     AEAEYRAKKPTEGAD"
     gene            complement(760..1230)
                     /locus_tag="SCJ86_002820"
     CDS             complement(760..1230)
                     /locus_tag="SCJ86_002820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286568.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEG1170302.1"
                     /translation="MSIISFILSTFLTGIKIKGMRPQLEVELNASYFAADMIYTKVII
                     SNYSTEPAMLVNLELSSANLNRRWSATPFKKLIAKGGSVDDNRIYSEAVPLNIPPKSA
                     ISCYLAFEVGKINFNKLLNHQTKMIFTLNRTQIHKIVDIKNTNFPIEKLVKELN"
     gene            1317..2015
                     /locus_tag="SCJ86_002821"
     CDS             1317..2015
                     /locus_tag="SCJ86_002821"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage regulatory protein"
                     /protein_id="HEG1170303.1"
                     /translation="MQELVILKNKEAVTTSLQVAESFEKKHQHVLRDIDALKKDVSNF
                     GQMFVEGNEPDSYGRNRRVFFISRDGFFLLAMGFTGKKAIYFKQKYIEAFNEMEDVIR
                     KNTVPQTIEDMMIYQLEEMKDVKKDVSMLKDTMRISGQQEFEIKQKGNMKVMGVLGGK
                     ESRAYEEISKKVFSKFWSEFKRTFSIPRYGELPRKRFDDAVSFIEMWLPETAIRMEID
                     QLNRQQRLFGDDNE"
     gene            2193..2534
                     /locus_tag="SCJ86_002822"
     CDS             2193..2534
                     /locus_tag="SCJ86_002822"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353384.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEG1170304.1"
                     /translation="MEKELSTLDQYLIDPDWGKPKIEETSGRKIRRNLLTNEELAWDQ
                     DDLGNHVTIWDHVYLIHLSKHSNKPEYIYVIEDGLIDALEEYDRDNLIDISYYGPGKK
                     YIAEMEAEFDE"
     gene            2527..>2816
                     /locus_tag="SCJ86_002823"
     CDS             2527..>2816
                     /locus_tag="SCJ86_002823"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340820.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1071 domain-containing protein"
                     /protein_id="HEG1170305.1"
                     /translation="MSEIKGTTNFEKLFSRKLNKILKKKGNFDYLSWAHAWEIMKKND
                     PQATVTINEYKHYRVVSGTHQDFLVEEYKPFLMDETGTYVSVSVTVKGHTET"
BASE COUNT         1035 a          406 c          566 g          809 t
ORIGIN      
        1 tcaacgaata ttagctaatt ttttagctta cagattattt ttttagaaag gagctatttt
       61 tatgtctgag aatttagact taaaaattcg agcggagatg agaaaaagaa gaatgacttt
      121 caaagaacta gctgcgctta tcggtatttc aggagcttat ttatcagata ttctaaacgg
      181 caatcgtgat ggaaagaaag cacaacagca tatcgaaaca gtgaaaaaaa tattggacat
      241 ccgatagggg ggtaggcgat gacaaaacta aaaaaacaag attttgtaaa aaaatacaat
      301 tattctccat ctacttatca acgtcgaatg tcggaactaa aaaatacagc aattttctca
      361 gcggcgtatg aacgggtcac aggacaagaa gtttggatca atacagaatt atacgataaa
      421 tttttgtctt tcaaatccta taacaggtta cgcacaagaa aggtaacacc taaagaattt
      481 atcgagaagc atttagttga tttataaaaa acagaacatt ttgagaggtg aaggttaatg
      541 ggtaaattca acagagcatt ggtattcagc gcaccgctaa tcatctacgc tttaggactt
      601 tggggaagca ggcaagcgtt gataggaacg attgtttaca tggtttggat ttttatgggg
      661 cttgatgaag ctgaagctga gtacagagcg aaaaagccaa ccgagggggc tgactaaaaa
      721 tgaatagaaa agagaaacta gaatgtatat tactattact cagtttaatt ctttcactaa
      781 tttctctatt gggaagtttg tatttttgat atcaacaatt ttgtggattt gggttctatt
      841 tagagtaaat atcatttttg tttggtgatt aaggagttta ttaaaattaa ttttccctac
      901 ttcaaatgca aggtaacaag atatagcact tttaggagga atatttagcg gtacagcttc
      961 agagtatatt ctattatcat cgactgatcc tcctttagca attaatttct taaatggagt
     1021 agctgaccat ctgcggttta agttagcgga tgaaagttct aaattaacta acatagctgg
     1081 ttctgttgaa tagttagaaa tgattacttt tgtataaatc atatctgcag cgaagtaaga
     1141 agcattaagt tcaacttcta gttgagggcg cattcctttt atttttatcc ctgttaaaaa
     1201 cgtactcaat ataaaactta ttattgacat ccattgaaaa attgttaagt gaaaaaaatt
     1261 caaaataaac accaccagtt tttaactaaa ttataccaaa aaggagagaa gaaataatgc
     1321 aagaattagt aattttgaaa aataaagaag ctgtgactac gagcttacaa gtggcagaaa
     1381 gcttcgagaa aaaacatcaa catgttttaa gagatattga tgcactaaaa aaagatgtgt
     1441 ccaattttgg acagatgttt gtagaaggta atgaaccaga ttcatatggc agaaatcgac
     1501 gagttttctt cattagtaga gatggatttt tcttgctggc tatggggttt acaggaaaga
     1561 aagctatcta cttcaaacaa aaatacattg aagcattcaa cgaaatggaa gatgttattc
     1621 gcaagaatac tgttcctcaa acaattgaag atatgatgat ctatcaatta gaagaaatga
     1681 aagatgttaa aaaagatgtt tccatgctta aagatactat gcgaattagc ggacaacaag
     1741 agtttgaaat taagcaaaaa ggaaatatga aagttatggg agttctaggg ggaaaagaaa
     1801 gccgagctta tgaagaaatc agcaaaaaag tattctcaaa attttggtct gaatttaaac
     1861 gtaccttttc aatcccaaga tatggcgagt tacctcgtaa gagattcgat gatgctgttt
     1921 catttattga aatgtggttg ccagaaactg cgatccgtat ggaaattgat caactgaaca
     1981 gacaacagag actttttggt gatgacaatg aatagagctg aagcgctaag aatagggacg
     2041 gcaattgcta attgctggtg gaaatactat aaaccaatca tcctaagcca acaacatatt
     2101 gacaagcaaa aagcatggca acaaataaaa aagtgactcc gccggcaagc aaagagtcac
     2161 aaagaaaaca catcataagg agattttagc atatggaaaa agaactttcc actctagatc
     2221 aatatttgat tgatcctgat tggggcaagc cgaaaattga ggaaacaagt ggtcgaaaaa
     2281 tcagacgaaa tcttttgacg aatgaagaac tagcttggga tcaagatgat ttaggcaacc
     2341 atgtaactat ttgggatcat gtttatctta tccatctatc gaagcattcg aataaacctg
     2401 aatatattta cgtcatcgaa gatggcttga ttgatgcgct agaagagtac gacagagata
     2461 acttgattga tatctcttat tacggaccag gtaagaaata cattgctgaa atggaggcag
     2521 aatttgatga gtgaaatcaa agggacaacg aactttgaaa aactttttag tcgtaagtta
     2581 aataaaattc tcaagaaaaa aggaaatttt gattatttat cttgggctca cgcgtgggag
     2641 attatgaaaa agaatgatcc acaggcaacg gtaactatta atgagtacaa acactacagg
     2701 gttgtttctg gaactcatca agactttctt gttgaggaat ataaaccttt tcttatggat
     2761 gaaactggga cttatgtatc tgtctcagta acggttaaag gacacacgga aaccga
//
LOCUS       DARBET010000148         2807 bp    DNA     linear   BCT 07-NOV-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4027
            SAMEA114569841-rid20112183.denovo.154, whole genome shotgun
            sequence.
ACCESSION   DARBET010000148 DARBET010000000
VERSION     DARBET010000148.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA114569841
            Sequence Read Archive: ERR12203666
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2807)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2807)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001972618.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001972618.1
            Genome Coverage       :: 132x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/07/2023 13:18:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,041
            CDSs (total)                      :: 2,975
            Genes (coding)                    :: 2,908
            CDSs (with protein)               :: 2,908
            Genes (RNA)                       :: 66
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 67
            CDSs (without protein)            :: 67
            Pseudo Genes (ambiguous residues) :: 0 of 67
            Pseudo Genes (frameshifted)       :: 26 of 67
            Pseudo Genes (incomplete)         :: 28 of 67
            Pseudo Genes (internal stop)      :: 20 of 67
            Pseudo Genes (multiple problems)  :: 6 of 67
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2807
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA114569841-rid20112183.denovo.154"
                     /isolate="VRE4027"
                     /db_xref="taxon:1352"
                     /country="Denmark"
     gene            779..1033
                     /locus_tag="SCJ86_002824"
                     /pseudo
     CDS             779..1033
                     /locus_tag="SCJ86_002824"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002405927.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation protein"
     gene            1103..1615
                     /locus_tag="SCJ86_002825"
     CDS             1103..1615
                     /locus_tag="SCJ86_002825"
                     /inference="COORDINATES: protein motif:HMM:NF013677.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HEG1170306.1"
                     /translation="MTNKNNERYSTELKATAIKKMMPPENRGIRQLSQTLGIPEATLY
                     SWRKKARIQGNTTPGTKKNVEKWSSEDKFLIVMESFSMNQVELAEYCRKKGLFKEQIE
                     AWKKTCLSANEQEENRTRELATELKEEKKQARQLEKELRHKEKALAEAAALLLLRKKA
                     HAIWGDQEEE"
     gene            1612..2649
                     /locus_tag="SCJ86_002826"
     CDS             1612..2649
                     /locus_tag="SCJ86_002826"
                     /inference="COORDINATES: protein motif:HMM:NF033516.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
                     /protein_id="HEG1170307.1"
                     /translation="MISSSDRKQAVELIQEANRNGARLTYACNELNISVRTYERWTRE
                     GTIAHDQRPLAKRPVPKNKLTDQEREKIIETVSKKEFMNLPPSQIVPKLADCSIYIAS
                     ESSFYRVLREKNMQHHRGRSQVSQKRIPPSHLATKPNEVWTWDITWLKGPIKGLFYRL
                     YLIIDLFSRKMVGWEIWESEEARHAEKLIKQTVLKERIQGQPLVLHSDNGSPMKAATF
                     QVLLEKLGIQSSYSRPRVSNDNPYSEAAFRTLKYRPEYPNNGFKTIDDARKWTKEFVD
                     WYNDSHLHSGIQFVSPAACHEGRHLAILEKRKQVYEDAKKRHPERWSKGVRNWTPHQS
                     VALNPLKETES"
BASE COUNT         1016 a          492 c          559 g          740 t
ORIGIN      
        1 atggcgatat ttgatgttga tactttatgc agtaccctat gtattcttcg ctatcattct
       61 gtggctcata gcattgtttt ttagagtatt taatgacctt taggtagatt tatatataaa
      121 aagggctaaa tacgcaaaat acccccttaa aatgcaaaat aaatgcaaat aagggggtaa
      181 ctccttatga aaaaacaact aatagtatta taacacgttt ttatgccgag aaaacttatt
      241 ggttggaatg ggctatgtgt tagctaactt gttagcgagt tggttggact tgaattggga
      301 ttaatcccaa gaaagtacca actcaacaac acataaagcc ctgtaggttc cgaccaataa
      361 ggaaattgga ataaagcaac gaaaggagtt gagtaaaatg agtgagaaaa cgaagaaaat
      421 cattcgagta tcattagctt tttcacaagt agtagtgcaa tcattgatag tttattatac
      481 ttaccagcaa tataaattat ctaaagagaa ttaagttttg attatgtaat aagttttggt
      541 actaaaaaag acttgatcta attagaccaa gtcttttgtt agtgttatat taataacaaa
      601 ataaaaataa gaagtcgctc actccccgac caaagtttgt gagcaaaata aacagtcgat
      661 gtttgtttct taattgtata tcttgatatt aaacgattta agattattct tcaagatata
      721 tattcgcaaa tgtaattgga aaagcgactt cttaaacaaa attaaggagt cgctttttat
      781 ggcaaaaata acgaaaaatg agcaagaaaa tttggtggaa aattacacaa aaaaaccggc
      841 gtactctaat agccggttag acgcacattt cgtgtgcact tctaatccga aattgagctt
      901 tgacgctatg acgatagtcg gaaaccttaa taaaaacagc gctaaaaagc tatccgattt
      961 tatgagttta gatccacaaa ttagactttg ggatatactt caaacaaaat ttaaagctaa
     1021 agcactacaa gaccccctat gttaggctag ttgtcgtata ggataataag ttataataaa
     1081 gactatacgg aggatgatta ccatgacaaa taaaaacaat gaacgttact ctaccgaact
     1141 gaaagcgaca gccatcaaga aaatgatgcc acccgaaaat agggggattc gccaattaag
     1201 tcaaactttg ggcattccag aagcgactct ttattcttgg cgtaaaaaag ctagaattca
     1261 agggaatact acacccggaa cgaaaaaaaa tgtcgaaaaa tggtcaagtg aagataaatt
     1321 tttaattgtg atggaatcat tctcaatgaa ccaagtagaa cttgcggaat attgccgaaa
     1381 gaagggctta tttaaagaac aaattgaggc ttggaagaaa acgtgcttat ctgcgaatga
     1441 acaggaagaa aatcgaacga gagaacttgc aactgaacta aaagaagaga aaaaacaagc
     1501 gcgtcaatta gagaaagaat tacgtcataa ggaaaaagca ttagcggaag ccgctgctct
     1561 cttattacta agaaaaaaag cacacgcgat ttggggggac caagaggaag aatgatttca
     1621 tcttcagatc gcaaacaagc tgttgaactt attcaagaag ccaatcgcaa tggggcccgt
     1681 ctcacctatg cctgtaatga gctgaatatt agcgttcgaa cctacgaacg atggacgcgt
     1741 gaaggtacga tagcgcatga tcaacgccct ttggcgaaac gtcctgtacc caaaaataaa
     1801 ctgacggatc aagaacgcga aaaaataata gaaaccgttt caaaaaaaga atttatgaat
     1861 ttgccaccta gccaaattgt accaaaactt gcggattgct cgatttatat tgcatcagag
     1921 tctagcttct atcgtgtatt gcgagaaaaa aatatgcaac atcaccgtgg ccgaagtcaa
     1981 gtttcacaaa aacgcatacc gccaagtcac cttgcgacga agccaaatga agtttggact
     2041 tgggatatta cttggctaaa aggtccaata aaaggactct tttaccggtt atatctcatc
     2101 attgatttat ttagccgtaa aatggtaggc tgggagattt gggaatcaga agaagctcgt
     2161 catgccgaaa aacttattaa acagacggtg cttaaggaac gtatccaagg acagccgttg
     2221 gttttacact ccgataatgg cagccctatg aaagccgcga cttttcaagt cttactcgaa
     2281 aaattaggaa ttcaaagttc ttattcaaga ccacgagtga gtaatgacaa tccttattcg
     2341 gaagcggctt tccgaacgtt aaaatatcga ccagaatatc ccaataacgg ctttaaaaca
     2401 atagatgatg ctcgaaaatg gacgaaagag tttgtcgatt ggtataacga ttctcatttg
     2461 catagtggga ttcaatttgt gtctcctgca gcgtgtcatg aaggacgaca tctcgctatt
     2521 ttagagaaac gaaagcaggt ttacgaggac gctaaaaagc gccatcctga acgctggtca
     2581 aaaggtgttc gaaattggac tccacatcaa tcggtggcgt tgaatccttt aaaagaaaca
     2641 gaaagttaac aggagcataa tctttcaaga ttatcaaata accctcgtca cccaataggg
     2701 gaaaaagtta ttcttcccct attggataac gaggccaacc gaccataggg aggttctgct
     2761 ttttgatagg tggaaaataa aaatacgaca actatattga caaacac
//
LOCUS       DARBET010000149         2739 bp    DNA     linear   BCT 07-NOV-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4027
            SAMEA114569841-rid20112183.denovo.155, whole genome shotgun
            sequence.
ACCESSION   DARBET010000149 DARBET010000000
VERSION     DARBET010000149.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA114569841
            Sequence Read Archive: ERR12203666
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2739)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2739)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001972618.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001972618.1
            Genome Coverage       :: 132x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/07/2023 13:18:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,041
            CDSs (total)                      :: 2,975
            Genes (coding)                    :: 2,908
            CDSs (with protein)               :: 2,908
            Genes (RNA)                       :: 66
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 67
            CDSs (without protein)            :: 67
            Pseudo Genes (ambiguous residues) :: 0 of 67
            Pseudo Genes (frameshifted)       :: 26 of 67
            Pseudo Genes (incomplete)         :: 28 of 67
            Pseudo Genes (internal stop)      :: 20 of 67
            Pseudo Genes (multiple problems)  :: 6 of 67
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2739
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA114569841-rid20112183.denovo.155"
                     /isolate="VRE4027"
                     /db_xref="taxon:1352"
                     /country="Denmark"
     gene            700..2610
                     /gene="ltrA"
                     /locus_tag="SCJ86_002827"
     CDS             700..2610
                     /gene="ltrA"
                     /locus_tag="SCJ86_002827"
                     /EC_number="2.7.7.49"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="group II intron reverse transcriptase/maturase"
                     /protein_id="HEG1170308.1"
                     /translation="MPKNKNETLCVEDLRHAEYYEMQNTFDDLYAKSKNGDIFTHLMD
                     IILSRENILLAYRNIKANAGSKTAGTDGTIIKDIGKLPAETVVKKVRYIVAGSPHGYR
                     PKPVRRKEIPKPNGKTRPLGIPCMWDRLIQQCIKQVLEPICEAKFSENSYGFRPNRSV
                     ENAIKATYNRLQISQLHYVIEFDIKGFFDNVNHSKLIKQIWAMGIRDKHLIFILKRIL
                     KAPIKMTNGTITYPEKGTPQGGIISPLLANIVLNELDHWVESQWQENPVTKNYVVHIN
                     KSGSPCKSNAYKEMKKTKLKEMYMVRYADDFRVFCRYKESAEKAKIAITQWIEQRLKL
                     EVSQEKTRIVNVRKRYSDFLGFKIKMIPRRKKLVVKSHISDKQFKNQKDKLVSQAKKI
                     ASPSKGKTELEETRLYNSMVLGMQNYYQIATNVNLDCMKLNRAVMAIFTNRLGTNKRG
                     RLRKTGRQLTTKEATRYGKSKMLRYVAGEGEPIFPVGYIQHRKPMAKVYLANCYTLEG
                     RKFIHTNLSVDKLLMYQLMKLSLENRTIEYADNRISLFSAQHGKCAITLEEFQQANEI
                     HCHHIVPTGAGGNDDYKNLILVKEAVHRLIHAKTEETIQKYIALLKLNKVQLIRLNKY
                     RVLAGNQELNLI"
BASE COUNT         1072 a          425 c          558 g          684 t
ORIGIN      
        1 aaaagctaag gcactaataa aagaacgaaa aagtttggtt attaggagaa ctgataatct
       61 gacaggtgga atgatgaagt aacgctctga aacgcctgcc ctgagcctcc gacaagcatt
      121 gtatgaggaa actcagaatg tttgaagctc ggtaaagacg gctgaaagat accgtaatag
      181 ttgatgaaca tatatcttct atcgaaaact atccagaggt gggtagcgta tccaacaggt
      241 cgggggtcta taaaatatct atggttagaa tgttctataa ggaactgacg aaacagcgaa
      301 tgtacgggtc taaatttgaa ctttgtagaa atgcaaaggt acgagaaatc gtagctggtg
      361 tggcaaagta agaatatagg ttatgaaatg ccatataacg ttacaggcgg tatcaagcca
      421 gcaggcttat agctagtgac ctaaggatat atatgtatag ataagattat cggaacgagg
      481 aaaggtattg ggttctcatc aagagaataa actgacgaag aacaataagc agttgaacta
      541 atgctgaaaa gcagagggcg aacctaagaa attcttgtaa tgagaatgga ggaatgccct
      601 caagtcgtgt atatattaga aaagtattat gttgtttcaa taaggattac gagtaagata
      661 aaaaagctca ctcaatttca agtaaaggag tgaagcctta tgcctaaaaa taaaaatgaa
      721 acattgtgtg tagaagattt aagacacgct gaatattacg aaatgcaaaa cacctttgat
      781 gatttatatg ccaaaagtaa gaatggagat attttcactc atcttatgga tattatccta
      841 tcgagagaaa atatattact ggcatatcga aatatcaaag caaatgcagg cagtaaaaca
      901 gcaggaacag acggtacaat aattaaagat attggcaaat tgccagcaga aacagttgtc
      961 aaaaaagtaa gatacattgt tgcaggcagt ccacatgggt atcgacctaa gccagtgagg
     1021 cgaaaagaaa taccaaaacc aaatggtaaa accagacctc taggtattcc ttgtatgtgg
     1081 gacaggctta tacaacaatg tataaaacaa gtattagaac caatctgcga agctaaattt
     1141 agtgaaaaca gctatggttt tagacctaat aggtcagttg aaaatgcaat aaaagcaacc
     1201 tataaccgcc tacaaatcag ccagctacat tatgtgattg aatttgatat taagggtttc
     1261 tttgacaacg tgaaccattc caagctgata aaacagatat gggctatggg aatacgggac
     1321 aagcacttga tattcatttt aaaaaggata ttaaaagctc caatcaaaat gactaacggt
     1381 acaataacat atccagagaa aggaactccg caagggggaa taatatcccc actactagca
     1441 aacattgttc ttaatgagtt agaccattgg gtagaaagcc aatggcagga aaatcctgtt
     1501 acaaaaaact atgtggttca tatcaataaa agcggtagcc catgtaaaag caacgcttac
     1561 aaagaaatga agaaaacaaa gttaaaggaa atgtatatgg ttcgttatgc agatgatttt
     1621 agagttttct gccgctacaa ggaaagtgct gaaaaagcca aaatagcaat cactcaatgg
     1681 attgaacaac gattaaaatt ggaagtatca caagaaaaaa caagaattgt aaatgttaga
     1741 aaaagatact ccgactttct tggtttcaag attaaaatga taccgagaag aaagaagtta
     1801 gttgttaaat cacatataag tgataaacaa ttcaaaaacc aaaaagacaa gctggtatca
     1861 caggcaaaga aaattgcttc tccctcaaaa gggaaaacgg aattagaaga aacaagactt
     1921 tataattcaa tggtattagg tatgcaaaat tattatcaga ttgcaacgaa tgtaaatctg
     1981 gattgtatga aactgaatag agctgtaatg gctatcttca ctaacagatt aggtacaaac
     2041 aaaaggggca gattgagaaa aacaggcaga caacttacga caaaggaggc aacaagatat
     2101 ggtaaatcta aaatgcttcg gtatgttgct ggcgagggcg aacctatttt ccctgttgga
     2161 tatatccaac acagaaaacc aatggcaaag gtgtatttag cgaattgcta cactctagag
     2221 ggaagaaaat ttatccacac caatctaagt gttgataaat tactgatgta ccagttaatg
     2281 aaattatctt tggaaaatcg cacaatagaa tacgcagaca atcgaatttc gctattttct
     2341 gctcaacatg gaaaatgtgc tataactctg gaagaatttc aacaagctaa tgaaatacat
     2401 tgtcatcata ttgtaccaac tggagctggt ggaaatgacg attataaaaa tcttattttg
     2461 gtaaaagagg cggtacaccg tctaatacac gctaaaacag aagaaacaat acaaaaatat
     2521 atagctctat tgaagctaaa taaagtccag ctcataagac taaataaata tagagtatta
     2581 gctggcaatc aagaattaaa cttgatatag gtctactaca taaatacttt tcaatataaa
     2641 cacgatggaa cgcggagtgc ggtgaaagtc gcacgctccg tgtgaatggg gggaaaagtt
     2701 tgagatattt ataatatcag gaacttacct atccatata
//
LOCUS       DARBET010000150         2683 bp    DNA     linear   BCT 07-NOV-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4027
            SAMEA114569841-rid20112183.denovo.156, whole genome shotgun
            sequence.
ACCESSION   DARBET010000150 DARBET010000000
VERSION     DARBET010000150.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA114569841
            Sequence Read Archive: ERR12203666
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2683)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2683)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001972618.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001972618.1
            Genome Coverage       :: 132x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/07/2023 13:18:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,041
            CDSs (total)                      :: 2,975
            Genes (coding)                    :: 2,908
            CDSs (with protein)               :: 2,908
            Genes (RNA)                       :: 66
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 67
            CDSs (without protein)            :: 67
            Pseudo Genes (ambiguous residues) :: 0 of 67
            Pseudo Genes (frameshifted)       :: 26 of 67
            Pseudo Genes (incomplete)         :: 28 of 67
            Pseudo Genes (internal stop)      :: 20 of 67
            Pseudo Genes (multiple problems)  :: 6 of 67
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2683
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA114569841-rid20112183.denovo.156"
                     /isolate="VRE4027"
                     /db_xref="taxon:1352"
                     /country="Denmark"
     gene            174..518
                     /locus_tag="SCJ86_002828"
     CDS             174..518
                     /locus_tag="SCJ86_002828"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629150.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxymuconolactone decarboxylase family
                     protein"
                     /protein_id="HEG1170309.1"
                     /translation="MAEKQTAGRDQLGDFAPKFAELNDDVLFGQVWSREELSAHQRSI
                     ITISALISGGNFEQLPAHLKIGKQNGITSEEIAEIITHLAFYVGWPKAWSAFSLAKEI
                     YLEENSEDKKEA"
     gene            519..938
                     /locus_tag="SCJ86_002829"
     CDS             519..938
                     /locus_tag="SCJ86_002829"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HEG1170310.1"
                     /translation="MTTKHEEIHNGVIFPAGEKNEAYAQVFTGQSYLNMLVNDPEVSV
                     GVGNVIFEPGCRNHWHVHKDGYQILLVTGGEGWYQEEGKPARRLMAGDVVVTHKGIKH
                     WHGATKDSWFEHLAITAGNSEFLEPVTDEVYEGLENQ"
     gene            complement(1030..1380)
                     /locus_tag="SCJ86_002830"
     CDS             complement(1030..1380)
                     /locus_tag="SCJ86_002830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330645.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEG1170311.1"
                     /translation="MEKELSKRIIDELLTYLYSHEITEIRIGVEFAEEGLFIEIQGTA
                     ETQPEDLDVLADLLNNPRDPSMEIYYQELLGNPRHDREDYHLLGSLIDDVEIMYAQPI
                     INIKIFRRASHSYY"
     gene            complement(1346..2566)
                     /locus_tag="SCJ86_002831"
     CDS             complement(1346..2566)
                     /locus_tag="SCJ86_002831"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330924.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1576 domain-containing protein"
                     /protein_id="HEG1170312.1"
                     /translation="MLSNTPTDNWSGLLTILTSPSNLLTDYFALGGFGSAFINVGILT
                     LLSVLLAYRHKVILNGPLFASILTVTGFSFFGKNLYNSISIILGVYLYAVFVNKPFSQ
                     YIMIGLFGSALSPVVSYITFGMRFPLLIGILLGNLAGIAIGLLLPPLAAQTLVFHRGF
                     TLYNIGFTSGLIAMTFTAVLRLFSYSIVENTLVFNEYHFPLIWIIFGFFSLTVGIGFY
                     YNSFRLLGIREIFDSSGKLTTDFIANSGIGATLINIGLVGLMLSSYVLLVGGQLNGPV
                     IGAILSAVGFSAFGCHLKNSFPILVGIFIASLFGTFHEITSTGMLVAAVFGTGLAPIS
                     GFYGSFYGVIAGVLHIALVHNVSTLHGGLNLYNSGFSTGFVAGILVPILDNFTAVRKE
                     KKTLGKRIIKKNHR"
BASE COUNT          998 a          485 c          523 g          677 t
ORIGIN      
        1 aaataaaaaa agcgttagca actatgctta aaaagcatat cgactgaata aactaagcat
       61 tggacaggct gaaagactac atttaaaatg aaatgcgaag aaacaataga aaataaagtt
      121 gccttagaac catagttgaa tcacctaaca gtcgtgaagg gagaataaga gaaatggcag
      181 aaaaacaaac cgcaggacga gatcaattag gagattttgc accaaaattt gcagaattga
      241 atgatgatgt tttatttgga caagtatggt ccagagaaga attatctgct catcagcgta
      301 gtatcatcac catatctgca ttaatttcag gaggaaattt tgaacaattg ccagctcatc
      361 tgaagattgg caagcaaaat ggcattacta gtgaagaaat agcagaaatc attacccatt
      421 tagcttttta tgtaggatgg ccaaaagctt ggtcagcttt tagtttagca aaagaaatct
      481 atttagaaga aaatagcgaa gataagaaag aggcgtaaat gacgacaaaa cacgaagaaa
      541 tacacaatgg cgtgattttt ccagcaggtg aaaaaaacga agcttacgct caagtattta
      601 ccgggcaaag ctatctgaat atgttagtga atgatcccga agtttctgtt ggtgtaggta
      661 atgtaatttt tgaaccaggt tgtcgcaatc actggcatgt ccataaagac ggctatcaaa
      721 tcttacttgt gaccggagga gaaggctggt atcaggaaga aggaaagcca gcaagacgat
      781 taatggctgg agatgtcgtc gtaacgcaca aagggattaa acattggcat ggtgcgacaa
      841 aagacagttg gtttgaacat ttggcgatca ctgctggtaa ttcagagttt ttagaacctg
      901 taacagatga agtgtatgaa ggattggaaa atcaataatg tcaagcttga acagctcctg
      961 aaagtagatt ttaatcgaac tttcaggagc tgcttttttg attcagtctt ttataaatag
     1021 aaaatagggt taataatagg aatgagaagc acgtcgaaag atcttaatat tgataatcgg
     1081 ctgggcatac atgatttcca cgtcatcaat caaactacct aacagatgat aatcttcccg
     1141 atcatgtcgg ggattaccta atagttcttg atagtagatt tccatactag ggtcccgagg
     1201 attgttcagc aaatcagcaa gtacatctaa atcttctggc tgagtttctg ccgtaccctg
     1261 tatctctatg aaaagccctt cctcagcaaa ttctactcca atacgtattt cagttatttc
     1321 atgggaatat aaataagtaa gaagctcatc gatgattctt tttgataatt ctttttccaa
     1381 gtgtcttttt ttcctttctg acagcggtaa agttatctag tatcggtaca aggatacctg
     1441 caacgaaacc agttgaaaaa ccactgttat atagatttag tcctccgtgc aaagtgctga
     1501 cgttatggac aagagcaata tgtagtacac cagctattac accgtaaaaa ctgccgtaaa
     1561 aaccgcttat gggcgcgaga cctgtcccaa acacagctgc aacaagcatg ccagtactgg
     1621 tgatctcatg aaaagttcca aacaaagaag caataaatat tccgactagt atcggaaaac
     1681 tgttttttaa atgacaacca aaagcactga aacccaccgc agataaaata gcaccgatga
     1741 caggtccatt taactgaccg ccaaccagca aaacataaga agacagcatc agaccaacta
     1801 agcctatgtt gatcaaagta gcaccaatac cagaattagc aatgaaatca gttgtcaatt
     1861 ttccagatga atcgaaaatt tctcgaatcc ccaaaagccg aaaggaatta taatagaaac
     1921 caattccgac tgtcagcgaa aaaaagccaa aaataatcca aatgagagga aagtgatatt
     1981 cattgaaaac caatgtattt tcgacgattg aatagctgaa taacctcaaa acagcagtaa
     2041 aagtcatcgc aatcaaccca gaggtgaatc caatattata aagtgtgaat ccacgatgga
     2101 aaactaaagt ttgagcagct aagggcggaa gaagtagacc aattgctatg ccagctaaat
     2161 tccccaataa aatacctatc agtaaaggaa aacgcatacc aaaagtgata taactaacga
     2221 ccggtgataa tgcgctacca aacagtccga tcatgatgta ctgagaaaaa ggtttattga
     2281 caaatacagc gtatagatac acacctaaaa tgattgagat agaattataa aggtttttgc
     2341 caaaaaaaga gaaaccggta actgttagaa tcgaagcaaa taagggaccg tttaaaatca
     2401 ccttgtgccg ataagctaaa agcacagaaa gtaaggtcaa aatccctaca tttatgaatg
     2461 cgcttccgaa accgcctaaa gcaaaataat ctgtcaaaag attgcttggc gaggtaagaa
     2521 tagttagcaa tccactccaa ttatcagtag gagtattaga aagcaagcca ataaaaagaa
     2581 aaaaacacct gtaaaaccaa gataaaaata ctttcttttc tgtgaaacga ttcgtttttg
     2641 aggatgaata taaataacgt cagtttcgtt ttccattaca cac
//
LOCUS       DARBET010000151         2502 bp    DNA     linear   BCT 07-NOV-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4027
            SAMEA114569841-rid20112183.denovo.157, whole genome shotgun
            sequence.
ACCESSION   DARBET010000151 DARBET010000000
VERSION     DARBET010000151.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA114569841
            Sequence Read Archive: ERR12203666
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2502)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2502)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001972618.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001972618.1
            Genome Coverage       :: 132x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/07/2023 13:18:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,041
            CDSs (total)                      :: 2,975
            Genes (coding)                    :: 2,908
            CDSs (with protein)               :: 2,908
            Genes (RNA)                       :: 66
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 67
            CDSs (without protein)            :: 67
            Pseudo Genes (ambiguous residues) :: 0 of 67
            Pseudo Genes (frameshifted)       :: 26 of 67
            Pseudo Genes (incomplete)         :: 28 of 67
            Pseudo Genes (internal stop)      :: 20 of 67
            Pseudo Genes (multiple problems)  :: 6 of 67
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2502
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA114569841-rid20112183.denovo.157"
                     /isolate="VRE4027"
                     /db_xref="taxon:1352"
                     /country="Denmark"
     gene            complement(1032..1322)
                     /locus_tag="SCJ86_002832"
     CDS             complement(1032..1322)
                     /locus_tag="SCJ86_002832"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295437.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEG1170313.1"
                     /translation="MEKKYGTVDKIRTLQVSARPLIRFSLNGVNCLIASHSLNFLAEV
                     DEGMKLVVTGYYNNRKQFVVRSYHVLGKPKIVVEYEKSLYPRKKVRQDERIN"
     gene            complement(1530..2222)
                     /locus_tag="SCJ86_002833"
     CDS             complement(1530..2222)
                     /locus_tag="SCJ86_002833"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318394.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit omega"
                     /protein_id="HEG1170314.1"
                     /translation="MKKKFITGGIVVAFLCLIGYTGTRYANEIILWGGETDIDTINEN
                     LTQLDHTLTDKEQALTDLTARLAQKEQTLQDSKADVQEYQTKVNELENQKNQSAAEKQ
                     NLESQLVSKDAQLQSKQNEINVKLDEINQKNDEINHLNADWTARLQEAQAKLSELQNT
                     VNGLNQQIATLTQEKTNVETQLTDTQKKLDEATAENSSLKQYIQKLEKAKDEVEDTAD
                     KSQQIVEEHLRK"
BASE COUNT          740 a          461 c          351 g          950 t
ORIGIN      
        1 atttattttc tcaaaaacat acttgcaaag taatactaca attcctaaca taatcgtaga
       61 atttaataat ttccatagca taaagtgttt agaaaagaac attaagaata gttctataat
      121 aattcttgaa ctccatgtct tataatgttc tatcgaagca cccaaatagc caagttcatc
      181 taatctactt gcataaacta cgtcatccgc tctcatagtc ggcatataca tattaaagag
      241 taaaaagata ccccaaatcc ctattagtaa caagatcagt tctttgttat tcgttatttt
      301 tctattatgt ttgttcattt ttatactcct tcttatattt cctaatagag tataacatag
      361 ctttaagcat tacttaaaga taacttaaaa gttatctttt aaatacttaa ttgttaaata
      421 ccttatttga tgcaaaagaa atctacgata gtcaacaaaa aaattttcat tatatccaga
      481 aaaatttttc ttagttaagg acctatggat tgcagtaatg cagaattcgt cgaataaact
      541 gccaaagaca tacaatcata ttgcttttaa aattgacgaa caagatatag attcgtttgt
      601 ctcaaaaatc caaatgttag gtttaactgt cgaaccagga cgttctcgtg tgaaaggcga
      661 ggcttcttca atttactttt acgactatga taatcacctc ttcgagttac ataccggtac
      721 tctccaggaa cgtttaaaat cctacaattc cactacctga gactagtaat gactagtctc
      781 tttttatggc ctctgatccc cccattgagc gaatactaat agggcttcga attgatctgt
      841 tcaatcttta agcaatatcc ctcaaatgag tttggaaagt ttagtgattc atcacaaata
      901 cttaaataca tgtagttata tatagattga ggttcttgtg agcgtatgct cgtgtgggtt
      961 caaatcccat cgtctgcatc catctttcca aaacaagcgg ctaggacatt tttgtctcag
     1021 ccgcttgttt tctagtttat tctttcatct tgtcggacct tttttcttgg gtatagactt
     1081 ttctcatatt ctaccacaat tttgggtttc cctaacacat gataggatct cacaacaaat
     1141 tgctttctat tattataata acctgtaacg actaacttca tcccttcgtc tacttcagct
     1201 aggaagttca aactatggga agcaatcaaa caattgaccc catttaaact gaaacggatc
     1261 aacggtctgg cactaacttg caaagtacgg atcttatcta ctgttccata ttttttctcc
     1321 ataaccttca cctcaatacc attatacgaa catacattcc ctttgtaaag aagaaactct
     1381 gttttatctt tttgactttt tgtctattac ctttcttaaa atcatattgg cacttcctgc
     1441 gaagatgaag gacatgtact aaaaaaaaga ggcagcagta tagattactg ttgcttcttt
     1501 actaatttag gtttacttgt taagaaagat tatttacgca aatgttcttc aacaatttgc
     1561 tgagatttat cagctgtatc ttccacttca tcttttgctt tttctaactt ttgaatatat
     1621 tgtttcaagc ttgagttttc agcagttgct tcgtctaatt tcttttgtgt atctgttaac
     1681 tgagtttcta catttgtttt ttcttgtgtc agtgttgcga tttgttgatt caaaccattt
     1741 acagtatttt gtaattctga aagttttgct tgtgcttctt gtagtcttgc tgtccaatct
     1801 gcattcagat gattgatttc atcatttttt tggttgatct catctagttt gacattgatt
     1861 tcattttgtt tgctttgtag ttgtgcatcc ttgctgacta attgactttc taagttttgt
     1921 ttttctgctg ccgattgatt tttttggttt tctaattcat ttacttttgt ttggtattct
     1981 tggacatctg cctttgaatc ttgcaatgtc tgttcttttt gtgctagtcg agcggtaaga
     2041 tctgtaagcg cttgctcttt atctgtcaat gtatgatcaa gctgtgtcag attctcattg
     2101 attgtatcaa tatctgtctc tccaccccac aaaataattt catttgcgta ccgcgtgcct
     2161 gtgtagccaa ttagacatag aaatgcaacg acaatcccac cagtaataaa tttctttttc
     2221 atttgcatag ctccttaatc caatactttt acatgctaaa agtaagtgat ctcaaacaca
     2281 atgtcaagcg tttttaaatt acatgtgtaa ttttaatata aatactatca aatactcatt
     2341 taatcctcct tttttatcta tttaaaaaat cttttttata gagaaaaatg caggttaata
     2401 ctttcttaaa taaatattta tggtgaacaa aaacaaaaaa gcaagtttta ttaaacgtaa
     2461 cccttttcat ttcatagatt actgtattta ttacaaaaaa cg
//
LOCUS       DARBET010000152         2436 bp    DNA     linear   BCT 07-NOV-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4027
            SAMEA114569841-rid20112183.denovo.158, whole genome shotgun
            sequence.
ACCESSION   DARBET010000152 DARBET010000000
VERSION     DARBET010000152.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA114569841
            Sequence Read Archive: ERR12203666
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2436)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2436)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001972618.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001972618.1
            Genome Coverage       :: 132x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/07/2023 13:18:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,041
            CDSs (total)                      :: 2,975
            Genes (coding)                    :: 2,908
            CDSs (with protein)               :: 2,908
            Genes (RNA)                       :: 66
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 67
            CDSs (without protein)            :: 67
            Pseudo Genes (ambiguous residues) :: 0 of 67
            Pseudo Genes (frameshifted)       :: 26 of 67
            Pseudo Genes (incomplete)         :: 28 of 67
            Pseudo Genes (internal stop)      :: 20 of 67
            Pseudo Genes (multiple problems)  :: 6 of 67
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2436
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA114569841-rid20112183.denovo.158"
                     /isolate="VRE4027"
                     /db_xref="taxon:1352"
                     /country="Denmark"
     gene            complement(<1..272)
                     /locus_tag="SCJ86_002834"
     CDS             complement(<1..272)
                     /locus_tag="SCJ86_002834"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341747.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEG1170315.1"
                     /translation="MINLGEWGMIAGSITAIVSLILLVIRPIAASFSKITETLSKVSH
                     NLDLLTKDLESSKSDRLMIHEELKKHDERLDTHTEKLVEHTQQIKT"
     gene            complement(309..446)
                     /locus_tag="SCJ86_002835"
     CDS             complement(309..446)
                     /locus_tag="SCJ86_002835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336488.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="XkdX family protein"
                     /protein_id="HEG1170316.1"
                     /translation="MFSFSDVKMMYDWGCFTDDQVRLFVPLCITDEEADKIINKDKSA
                     S"
     gene            complement(448..894)
                     /locus_tag="SCJ86_002836"
     CDS             complement(448..894)
                     /locus_tag="SCJ86_002836"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334691.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEG1170317.1"
                     /translation="MKNIWKYGRTGGEYAGKVLDDMLVSVPYTDQPPLEGVRSDGEPL
                     TIADQMFDPKLNQWIILANALDHNDLNNLKAMYEALEHENGNLKQLNAKLMLSDVAIK
                     QENTALKEKADSLAQINSKMMLASLQNSKDISEIKEQLNPASKGGE"
     gene            complement(891..1040)
                     /locus_tag="SCJ86_002837"
     CDS             complement(891..1040)
                     /locus_tag="SCJ86_002837"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290627.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEG1170318.1"
                     /translation="MKVVYKSIKPYGFEQIIVNDEEHLPEECTEVEPPIPNWKPKFNY
                     WEGNK"
     gene            complement(1054..>2436)
                     /locus_tag="SCJ86_002838"
     CDS             complement(1054..>2436)
                     /locus_tag="SCJ86_002838"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305370.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEG1170319.1"
                     /translation="GKPNLMSKLKSSDFNVGYHGSLTSDNEKLHFTSDGTGSIIMFTR
                     INTPQLASGKTYTLSAKVRFDEGTTGAIDKLRLVYRTSPGEKILLEANSTNITTDDVG
                     KEITIKGTANVNYQITNLDRFYMSISFVDRDKINGGFKLYDIKIEEGSTATPYQPNLL
                     DDPYWVGKTSLGENIANKGTTFPIKSSAYEIYKGNTEEELIIGQTYTITLKGTKPASQ
                     TFVAYNYWNVNFGELKPVEGLTDVWSLTFTPTKLEPGLPKDLRIFQLPKETAGACQID
                     WLKIEKGDTRTPNISQFKYFGEGLKDSNNPNDYSWDITPEYTEKGLNDTVSLTEPQSI
                     EGLKNFEDGIQSKGKSVLTSDDNKYEVVTLTVTNGNTGSAKLYREGKTVTIYFFALNG
                     KSSGGNDSTILTIPEGYRPPISFEQLVGSIDRSTLNSAQLSIGADGAIKWRRNSSYGS
                     AYSFVITYSI"
BASE COUNT          638 a          502 c          420 g          876 t
ORIGIN      
        1 aaagttttga tttgttgcgt gtgttccacc aatttttctg tatgtgtatc taatctttca
       61 tcgtgtttct ttagttcttc atgaatcatc aatcgatctg atttgctcga ttctaaatct
      121 ttagtcagca aatctaaatt gtggcttact tttgaaagag tctcagtaat tttcgagaaa
      181 gatgcagcaa tcggtctaat tactaataaa atcaaagaaa cgatagcggt tattgatcct
      241 gctatcattc cccattcccc taaattaatc atgtgacaac tcctttacct taaataaaaa
      301 cgcatcaatt aagatgcgct cttatcttta ttaatgattt tatctgcttc ttcgtctgta
      361 atgcatagtg gaacgaatag tcgaacttga tcgtcagtaa aacagcccca atcatacatc
      421 attttcacat cgctaaaact aaacatacta ctcacctcct tttgaagctg gatttagttg
      481 ctctttaatt tctgaaatgt ctttgctatt ttgtaacgaa gcaagcatca tttttgaatt
      541 gatttgtgct aaactgtcag ctttttcttt caatgcagta ttttcctgtt taatcgctac
      601 atcgcttagc atgagtttgg catttagctg ttttaggttg ccgttttcat gttcaagagc
      661 ctcgtacatc gctttgagat tgtttaaatc gttgtgatct aacgcgttcg ctaaaataat
      721 ccattggttc aatttaggat caaacatctg atcagcaatc gttagcggtt cgccatctga
      781 acgaacccct tcgagcggtg gctgatctgt gtaaggaacg gatacaagca tgtcgtccaa
      841 tacttttcct gcatactctc cgccagtacg gccatatttc caaatgtttt tcatttattt
      901 ccctcccagt aattgaattt cggtttccaa tttggaatcg gtggttcgac ttctgtgcat
      961 tcttccggta aatgttcttc gtcattcaca atgatttgct cgaatccgta aggcttgatt
     1021 gatttgtaaa ctactttcat tagatttcct cccttaaatt gaataagtga taacaaatga
     1081 ataagctgat ccataacttg agtttcttcg ccatttaatg gctccatctg caccaataga
     1141 taactgagca ctgttcaaag tagaacggtc tatcgagcca accagttgct caaaactaat
     1201 tggtggccga tagccttctg gaattgttag tatcgttgaa tcatttccac cactgctttt
     1261 cccattcaac gcaaaaaaat aaatagtgac ggtttttcct tcacgataaa gctttgctga
     1321 tccggtattc ccgtttgtaa ctgttaaggt tacgacttca tatttgttgt cgtctgatgt
     1381 caatacagat tttccttttg attgaatacc atcttcgaaa tttttcaatc cttcgatgga
     1441 ttgcggttca gtcaaactaa ccgtatcatt caagcctttt tcagtatatt caggcgtgat
     1501 gtcccagctg tagtcgttcg gattgttgct atctttcaat ccttcaccaa agtatttaaa
     1561 ctgactaata ttcggggttc gggtgtcgcc tttttcaatt ttgagccagt caatttggca
     1621 tgcgccggcc gtttctttag gcaactgaaa aatgcgaagg tctttaggca aacccggttc
     1681 aagtttcgtt ggtgtgaatg ttagagacca tacgtctgtc aatccctcaa ctggttttag
     1741 ttctccaaaa ttaacgttcc aataattata tgctacaaag gtttgacttg cgggttttgt
     1801 tcctttaagc gtgatagtat acgtttgacc tattataagc tcttcttccg tgttaccttt
     1861 atatatttcg tacgcgctag atttaattgg gaacgtcgta cctttatttg caatattctc
     1921 acccaacgat gttttaccta cccagtaagg gtcatctagt aagttaggct ggtacggtgt
     1981 ggctgttgag ccttcttcga ttttgatgtc gtacaactta aatccgccat ttattttatc
     2041 cctgtcaaca aagctaatac tcatataaaa tcgatctaaa tttgtgattt gataattaac
     2101 gttagctgta cctttgattg ttatttcttt ccctacatca tctgttgtaa tatttgtact
     2161 atttgcttcc aataatatct tttctcctgg tgatgtacga tacaccaaac gtaatttatc
     2221 aatagctcct gtagttcctt catcaaatcg aactttcgca ctcagagtat aggttttccc
     2281 actagcaagc tgaggtgtat taatacgcgt aaacataata atgcttcctg taccatcaga
     2341 agtaaaatgt agcttttcgt tatccgaagt tagtgacccg tggtaaccaa cgttaaaatc
     2401 gctcgatttt agtttggaca ttaaattcgg cttacc
//
LOCUS       DARBET010000153         2427 bp    DNA     linear   BCT 07-NOV-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4027
            SAMEA114569841-rid20112183.denovo.159, whole genome shotgun
            sequence.
ACCESSION   DARBET010000153 DARBET010000000
VERSION     DARBET010000153.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA114569841
            Sequence Read Archive: ERR12203666
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2427)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2427)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001972618.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001972618.1
            Genome Coverage       :: 132x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/07/2023 13:18:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,041
            CDSs (total)                      :: 2,975
            Genes (coding)                    :: 2,908
            CDSs (with protein)               :: 2,908
            Genes (RNA)                       :: 66
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 67
            CDSs (without protein)            :: 67
            Pseudo Genes (ambiguous residues) :: 0 of 67
            Pseudo Genes (frameshifted)       :: 26 of 67
            Pseudo Genes (incomplete)         :: 28 of 67
            Pseudo Genes (internal stop)      :: 20 of 67
            Pseudo Genes (multiple problems)  :: 6 of 67
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2427
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA114569841-rid20112183.denovo.159"
                     /isolate="VRE4027"
                     /db_xref="taxon:1352"
                     /country="Denmark"
     gene            123..2276
                     /locus_tag="SCJ86_002839"
     CDS             123..2276
                     /locus_tag="SCJ86_002839"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320418.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding protein 2"
                     /protein_id="HEG1170320.1"
                     /translation="MMKDFMKKILNTSWLEKIRKGAQEPKNKTFRRSHVPFRLNFLFF
                     IIFTLFVALIARLGYLQIVNGEEMEARVKSTSTITVQESTPRGMIYDSTGKALVTNKA
                     NQAITYTRGTQVTAKDLLEIATKLNSLIDVPVDENLTDRDKKDFWLADPKHLKEATKR
                     LSAKEKQLGNSEQYSAMVNKVTADEINLNEEQLKIATIFKRMNSAYELNTVYIKNSGV
                     TDQELAVVAERASELPGVSTGTDWTREYNAASSLKSILGSVTTEKQGLPADEAEKYLA
                     KGYSRNDRVGQSYLEKQYEDVLQGTKTQYEVSLDNEGNVSNQKEIFSGEKGSNLMLSM
                     NAEFQSKVEEILKRNYQTLINNGKAQYSPGAYAVAMNPQTGEVLAMTGFSHEQGSKEI
                     TENALGTITSAFDPGSVVKAGTLTAGWASNAISGNQVLIDEPIRLQGASEKSSVFNRS
                     GQVALDAVKASELSSNTYMIKVALKMLGLDYTPGMGLPSLDEEAKAYQQLRDSFKEFG
                     LGTTTGIDLPNESPGISRSVDYMKKFNADNGKEWYAPGNFTDLAFGQFDTYTPIQLAQ
                     YASTVANGGKRVQPHLVKAIYGNDENGNLGEVKKEIGTTVENTVNISAENMAILREGF
                     HQVVHGTDPYTTAKPLASAKMDLSAKTGTAETVAEGHPDIATVNSNIVAYGPTDNPEI
                     AISVVLPNLLDEQDHINLTIAKEIMDAYYDMFMAK"
BASE COUNT          829 a          426 c          537 g          635 t
ORIGIN      
        1 caaaaagttg attttatctt gaaattaggt aaagaatttc ttgctccgcc cattttttct
       61 tttttattaa gaagtttttg ggtacaatga agcatgaatg taaataggcg gaaggatagg
      121 aaatgatgaa agattttatg aaaaaaatat taaatacttc ttggttagaa aaaataagaa
      181 aaggtgcaca agaacctaag aacaaaacgt ttcgccggtc ccatgttcct tttcgcttaa
      241 attttctttt tttcattata tttacgttat ttgttgcgtt gattgctaga ttgggttatt
      301 tgcagatcgt gaacggcgaa gagatggagg cgcgagtgaa gtctacttct acgattacgg
      361 tccaagaaag tactcctcgc ggaatgatct acgactccac aggaaaagct ttagttacga
      421 ataaagcgaa tcaggccatc acatatacga gaggaacaca agttacggct aaagatttgc
      481 ttgaaatcgc aacgaagctg aactcgctga tcgatgttcc ggtagatgaa aatctgacag
      541 atagagataa aaaagatttt tggctggctg atcccaaaca tttgaaagaa gccacaaaaa
      601 gactttcggc aaaagaaaag caattaggga acagcgaaca atatagtgcg atggtcaata
      661 aagtcacagc tgatgagatt aatttaaacg aggagcaatt aaaaattgct accatcttta
      721 aacggatgaa tagtgcatat gaattaaaca ctgtctatat caaaaactct ggtgttactg
      781 atcaagagtt agctgttgtg gcagaacgcg cttctgaact tccaggagtt tcgactggca
      841 cagactggac acgagaatat aatgcagcaa gttcactgaa aagtattctt ggcagtgtga
      901 cgactgaaaa gcaaggactt ccagcagatg aagcagagaa atacctagca aaaggttatt
      961 ctcggaatga ccgtgtagga caaagttatt tggaaaaaca atatgaagat gtcctgcaag
     1021 gaaccaaaac ccaatatgaa gtttcccttg ataatgaagg aaatgtttcg aatcaaaaag
     1081 aaatcttctc tggggaaaaa ggttctaatt taatgttatc tatgaatgca gaattccaat
     1141 caaaagtaga agaaattttg aaaaggaatt atcaaacatt gatcaacaac ggcaaagcac
     1201 agtattcgcc tggtgcttat gcagtggcta tgaatcctca gacaggagaa gtgctagcaa
     1261 tgactggttt cagtcatgaa caaggttcca aagaaatcac cgagaatgct ttaggcacga
     1321 ttactagcgc ctttgatcca gggtctgtag tcaaggctgg tactttgaca gcaggctggg
     1381 caagtaacgc gatcagcgga aatcaagttt tgattgatga gccgattcgt ctgcaaggtg
     1441 cttcagaaaa aagctctgtc tttaatcgaa gcggacaagt tgctcttgat gcagtcaaag
     1501 catcggaatt gtcttctaat acgtatatga tcaaagtggc gttaaagatg ttaggactag
     1561 actatacacc tggaatggga ctacctagtt tagatgaaga agctaaagct tatcagcaat
     1621 tgagagactc tttcaaagag tttggtcttg gtacaacgac tgggatcgat ttgccgaatg
     1681 aatctcctgg gatctcacgt tctgttgatt atatgaagaa gttcaatgcg gataatggca
     1741 aagaatggta tgctccaggt aactttaccg atttggcatt tggacagttt gacacatata
     1801 caccaatcca attagctcaa tatgcttcaa cagtcgctaa tggcgggaaa cgagtacagc
     1861 cgcacctagt caaagcgatc tatggcaatg acgaaaatgg aaacttgggt gaagtgaaga
     1921 aagaaattgg cacaacagta gaaaatacgg tcaatatttc tgcagaaaat atggcgatcc
     1981 tccgtgaagg attccaccaa gttgtccatg gtaccgatcc ttacacaaca gccaaaccat
     2041 tagccagtgc gaaaatggac ctttccgcaa aaacagggac tgcagaaacg gttgcggaag
     2101 gtcatccaga tattgcgaca gtcaatagta atatcgtggc atacggacca acagacaatc
     2161 cggaaattgc catcagtgtt gtcttgccga acttattaga cgaacaagac cacataaact
     2221 tgacgattgc taaagaaata atggacgcgt attatgatat gttcatggca aaataaaaaa
     2281 gaggtcgtga aaaagccgct tagtttcgag tatgaagaaa aattttgtga aaatagcttc
     2341 tcacattttt gcttaaattt gacttcatac cgagaaactg gcttttgaac accgtttact
     2401 cggcaaaaaa ggttgtgaca aaaattg
//
LOCUS       DARBET010000154         2407 bp    DNA     linear   BCT 07-NOV-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4027
            SAMEA114569841-rid20112183.denovo.160, whole genome shotgun
            sequence.
ACCESSION   DARBET010000154 DARBET010000000
VERSION     DARBET010000154.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA114569841
            Sequence Read Archive: ERR12203666
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2407)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2407)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001972618.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001972618.1
            Genome Coverage       :: 132x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/07/2023 13:18:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,041
            CDSs (total)                      :: 2,975
            Genes (coding)                    :: 2,908
            CDSs (with protein)               :: 2,908
            Genes (RNA)                       :: 66
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 67
            CDSs (without protein)            :: 67
            Pseudo Genes (ambiguous residues) :: 0 of 67
            Pseudo Genes (frameshifted)       :: 26 of 67
            Pseudo Genes (incomplete)         :: 28 of 67
            Pseudo Genes (internal stop)      :: 20 of 67
            Pseudo Genes (multiple problems)  :: 6 of 67
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2407
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA114569841-rid20112183.denovo.160"
                     /isolate="VRE4027"
                     /db_xref="taxon:1352"
                     /country="Denmark"
     gene            5..1150
                     /locus_tag="SCJ86_002840"
     CDS             5..1150
                     /locus_tag="SCJ86_002840"
                     /EC_number="2.5.1.48"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328567.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cystathionine gamma-synthase"
                     /protein_id="HEG1170321.1"
                     /translation="MHIQTKLIHGGISEDPTTGAVSVPIYQTSTYRQDGVGQPKQYEY
                     SRSGNPTRFALEELIADLEGGVRGFAFSSGLSGIHAVFSLFQAGDHILLGDDVYGGTF
                     RLFDKVLTKNGLEYTIIDTSNLDKIEQSIKPNTKALYLETPSNPLLKITDLEKSATLA
                     HQHGLIVIADNTFATPYFQRPLDLGSDIVVHSGTKYLGGHSDVVAGLVTSNHKDLADQ
                     IGFYQNAIGAVLGPQDSWLLQRGIKTLSVRMEEHQKNAFVVADFLFSHPAVEKVYYPG
                     LPDHELHGVAKQQMSGFSGMISFTLKNEESAIPFVESLQLFTLAESLGGVESLVEIPS
                     VMTHASIPKEKREEAGIKDGLIRLSVGIEYGQDLINDLAQAFDRIKN"
     gene            1321..1779
                     /locus_tag="SCJ86_002841"
     CDS             1321..1779
                     /locus_tag="SCJ86_002841"
                     /EC_number="4.4.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005879048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S-ribosylhomocysteine lyase"
                     /protein_id="HEG1170322.1"
                     /translation="MRMNVESFNLDHTKVKAPYVRVADRKTGAHGDVIIKYDVRFKQP
                     NKEHMDMPSLHSLEHLTAELIRNHADYIVDWSPMGCQTGFYLTVLNHESYEDILDVLE
                     ATMKDVLEATEVPASNETQCGWAASHTLEGAQALAREFLAKRDEWSEVEA"
     gene            1938..>2407
                     /locus_tag="SCJ86_002842"
     CDS             1938..>2407
                     /locus_tag="SCJ86_002842"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344587.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione peroxidase"
                     /protein_id="HEG1170323.1"
                     /translation="MSIYNFETTLEDGTVYSLKKYAGKPIIIVNTATKCGLAPQFKQL
                     EELYQAYQTQGLVILGFPSNQFKQELDSATEAAEACKTTYGVTFPMHQLIDVNGENTD
                     PLFTYLKNEAPGTVTKAIKWNFTKFLIDRNGKVIERFAPQTSPLKMTAAIESVL"
BASE COUNT          802 a          452 c          455 g          698 t
ORIGIN      
        1 aaacatgcac attcaaacaa aattgatcca cggaggcatc agtgaagatc ccacaacagg
       61 agctgttagt gtacctattt atcaaacatc cacttatcgg caagatggcg tcggccagcc
      121 taaacaatat gaatattcaa gatctggtaa tcctactcgt tttgcattgg aagaactgat
      181 tgctgatttg gaaggcggtg ttcgaggatt cgctttttct tctggtcttt ctgggattca
      241 tgccgttttt tctctttttc aagctggcga tcatatctta ttaggagatg atgtctatgg
      301 aggaacattc cgtctatttg acaaagttct tactaaaaat ggtctcgagt acacgatcat
      361 agatacgagt aatcttgata aaatagaaca atccatcaaa cccaatacaa aagcacttta
      421 tcttgaaaca cctagcaatc cgcttctaaa gattactgat ttagaaaaaa gtgccactct
      481 ggctcatcaa catggattga tagttattgc tgacaataca tttgctactc cttattttca
      541 aagaccttta gatctcggtt ctgatatcgt tgtccatagc ggtaccaaat atctaggcgg
      601 acacagcgat gtagtagctg gtttagtgac ttctaaccat aaagatctag ccgatcagat
      661 cggattttac caaaatgcta tcggagccgt acttggtccc caagatagct ggttacttca
      721 gcgcggaatc aagacactaa gtgttcgtat ggaggaacat caaaaaaatg cttttgtggt
      781 agctgatttt ctattttctc atccagcagt agaaaaggtc tactatccag gattacccga
      841 tcatgaactc catggagttg ctaagcagca gatgagcggc tttagcggaa tgatttcttt
      901 tactttaaaa aatgaagaat ctgctattcc atttgttgaa tcactccaac tatttacttt
      961 agcagaaagt ttaggtggcg tagaaagttt agtggaaatt ccttctgtca tgactcatgc
     1021 gtctattcca aaagaaaaac gagaagaggc tggcataaag gacggcttga tccgtctttc
     1081 tgtaggaatt gagtatggtc aggatctcat aaacgattta gctcaggcat ttgacagaat
     1141 caagaactag aagtcccaat aaacgactta gcagaaaaat ctaaaaaata tttcagattg
     1201 ctacaccttg gaaacgataa gtgttagaat caagtagaaa gacgtttttg taaatttcac
     1261 taaataattc tttagtgaaa agcatcaaaa cgaatgaaca aacagatagg agtcttacaa
     1321 atgagaatga atgtcgaaag ttttaatctt gaccatacaa aagtcaaagc accttatgta
     1381 cgtgtagctg atcgaaaaac gggtgcgcat ggcgatgtca tcattaaata cgacgtccgc
     1441 tttaaacaac caaataaaga acacatggac atgccaagtc tacattcact tgagcacttg
     1501 actgccgaac taatccgcaa tcacgcagat tacatcgtag actggtctcc aatgggatgc
     1561 caaacaggct tttacttgac tgtattgaat catgaaagct acgaagacat tttagatgtt
     1621 cttgaagcaa caatgaaaga tgttcttgaa gctactgaag tacctgcaag caatgaaaca
     1681 caatgtggct gggctgccag ccatacacta gaaggtgcac aagctttggc tcgtgaattt
     1741 ttagcaaaac gcgatgaatg gtctgaagta gaagcatagt ttctaaaaga acgtaaagat
     1801 gagaagcaat atagctatct tatcttcgta ttttattaaa tatttattgt ctgaaaagag
     1861 ttctaaacgg gaaaacaaat agctagtttt cagtttagcg ctctttttat tatataaaat
     1921 agaaaggaga aaactttatg tctatttata attttgaaac tactttagaa gacggcactg
     1981 tctattcatt gaaaaaatat gcaggcaaac cgataatcat tgttaacact gcaacaaaat
     2041 gcggtctagc acctcaattt aaacaattag aagaacttta ccaagcttat cagacacaag
     2101 gtctggttat cttaggcttt ccttccaatc aatttaaaca agagctagat tcagcgactg
     2161 aagcagcaga agcttgtaaa accacttatg gcgttacctt ccctatgcat caattgattg
     2221 acgtaaatgg agaaaataca gatcctcttt ttacttatct taaaaatgaa gctcctggta
     2281 ctgtcactaa agcaataaaa tggaatttta ctaaattttt gattgataga aatggcaaag
     2341 taatcgaacg atttgcacca caaacttctc cattgaaaat gaccgctgca attgaatccg
     2401 ttcttta
//
LOCUS       DARBET010000155         2303 bp    DNA     linear   BCT 07-NOV-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4027
            SAMEA114569841-rid20112183.denovo.161, whole genome shotgun
            sequence.
ACCESSION   DARBET010000155 DARBET010000000
VERSION     DARBET010000155.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA114569841
            Sequence Read Archive: ERR12203666
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2303)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2303)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001972618.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001972618.1
            Genome Coverage       :: 132x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/07/2023 13:18:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,041
            CDSs (total)                      :: 2,975
            Genes (coding)                    :: 2,908
            CDSs (with protein)               :: 2,908
            Genes (RNA)                       :: 66
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 67
            CDSs (without protein)            :: 67
            Pseudo Genes (ambiguous residues) :: 0 of 67
            Pseudo Genes (frameshifted)       :: 26 of 67
            Pseudo Genes (incomplete)         :: 28 of 67
            Pseudo Genes (internal stop)      :: 20 of 67
            Pseudo Genes (multiple problems)  :: 6 of 67
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2303
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA114569841-rid20112183.denovo.161"
                     /isolate="VRE4027"
                     /db_xref="taxon:1352"
                     /country="Denmark"
     gene            110..1972
                     /locus_tag="SCJ86_002843"
     CDS             110..1972
                     /locus_tag="SCJ86_002843"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336367.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PRD domain-containing protein"
                     /protein_id="HEG1170324.1"
                     /translation="MQQLTKRERQLLIYLVEMSEPVTIKKLAEKENVSVRTIKYDLDD
                     IREWLNERKQVLYSKRSQGIWMNISSSDRIKLKSELMDVNRFEIFADQSLRIKRLLIM
                     LLVTKEAITGMKMANALDVSKDTIMNDLDVLENNFLKEGLTLNRQARKGFWIIGEERL
                     VRLTIEEILQKEFTDYDIYKLMSLLLNGGETEYFEMYSATATPIQEVFNQVIIRMRHL
                     LEFENLEKLNYAELLNILIRVTIATVRLRKEATIGRYQLVAEQELDKNDLSYRVMQQV
                     FDYYQLPLFEDEYHYIYSDTFDTIHQQDVMKLVENVIYKVSKAINHSFYQDPQLLTNL
                     YAHLSMRLSRKKKFVNEYNPFKEDIKQKYPELFDAIKQAVRQEISEQNILINDSFVAY
                     IALHFLVSYEREEDLRSVRAVYICSTGLGVTSLIKQKINEELNNITIVAFASVLNAQE
                     MIATKDPDLVISIFPINGIDRPFIKVHPLPTEQDLKKIQQIIKKILASSNQQERHNLN
                     LRETSINKDTLEDFSKELILKAYTIYERLLVILEAELIQEYREAFLLHVFLMVHRITF
                     DQQYMAEGRQVDQIILDKGTLVGKIEAVFAENNLGINQSEISALFSYIKEGASS"
     gene            1972..>2303
                     /locus_tag="SCJ86_002844"
     CDS             1972..>2303
                     /locus_tag="SCJ86_002844"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306983.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PRD domain-containing protein"
                     /protein_id="HEG1170325.1"
                     /translation="MKMSDEALKIIEESQNREKLKQVIDLIEERLVEHAIVPTELQWT
                     ILINHLNEMLKRSQRKETIPVVDRDLFKEVSKEAITISDEIVRSIGNLTDDEIYVLSI
                     HFETAKNN"
BASE COUNT          827 a          342 c          453 g          681 t
ORIGIN      
        1 tctgtaatat tagttaaaaa gctataagtt gcacttagtt aagtgcaaac atttcatatt
       61 gtcgttagtt tagaaaacgc ttatattagc aatgtaagga gagatgataa tgcagcagct
      121 aacaaaaaga gaaaggcagc ttttgattta cttggtggaa atgtctgaac cggtaacaat
      181 caaaaagttg gctgaaaaag aaaatgttag tgttcggacg ataaaatacg atctagatga
      241 tataagagag tggctgaatg agcggaagca agtcctatat agcaaacgta gtcagggaat
      301 ttggatgaat attagtagct ctgatcgaat caaattaaaa agcgaattga tggatgttaa
      361 tcgttttgaa atatttgcag atcaaagtct ccgaatcaaa cgtttactaa ttatgctttt
      421 agtaacaaaa gaagctatta ctggaatgaa aatggccaac gcattagatg tcagcaaaga
      481 tacgatcatg aatgatttag atgtgctgga aaataatttt cttaaggaag gattgacctt
      541 gaatcgtcag gcgcgaaagg gtttttggat cataggagaa gaacgtctag ttcgattaac
      601 gattgaagag attcttcaaa aagagttcac ggattatgac atttataaat taatgtcatt
      661 gttattgaat ggcggtgaga ctgaatactt tgaaatgtat tctgctactg ctacacctat
      721 ccaagaggta tttaatcaag taatcattcg aatgcgacac ttactggaat ttgaaaatct
      781 agagaaactg aattacgcgg aattactaaa tattctgatt cgagtgacaa ttgctaccgt
      841 tcgcttacgc aaagaggcta cgattggtcg ttatcaattg gttgctgaac aagaattaga
      901 caaaaacgat ctttcttatc gcgtcatgca gcaggtcttt gattattatc agttgccact
      961 gttcgaggat gaatatcatt atatctatag tgatacattc gacacgattc accagcaaga
     1021 cgtcatgaaa ctagttgaga atgtgattta taaagtaagt aaagcaatca accattcttt
     1081 ttatcaagat ccacaattgt tgacgaatct gtatgctcat ttatccatgc gattatcgag
     1141 aaagaaaaaa tttgtcaatg aatataatcc ttttaaagaa gatatcaagc agaaataccc
     1201 ggaattattt gatgcgatca agcaagcagt tcgacaagaa ataagtgaac aaaatattct
     1261 gatcaatgat tctttcgtgg catatattgc cttacacttt ttggtttcct acgaaagaga
     1321 agaagatcta cgaagcgtta gagccgtcta catttgttca acagggttgg gtgtgactag
     1381 tctgatcaag caaaagatca atgaagaatt aaataatatc actatcgtgg catttgcttc
     1441 agtattgaat gcgcaagaaa tgattgctac caaagatcct gatttggtga ttagtatttt
     1501 tccaattaat ggcatcgatc gtcctttcat caaagtccat ccattaccca ctgaacagga
     1561 cttgaaaaaa attcaacaaa taataaaaaa aatacttgct tcttccaatc agcaggaaag
     1621 gcataacttg aatcttagag aaacgtccat caataaagat actttagaag actttagcaa
     1681 ggaattgatt ttgaaagctt atacgattta tgaacgttta cttgtcattt tggaagcaga
     1741 attgatccaa gaatatcgag aagctttttt gcttcatgta ttcttaatgg ttcatcgtat
     1801 cacatttgat caacaatata tggcagaagg tcgtcaagta gaccagatta ttttagacaa
     1861 aggaacactg gtaggaaaaa tcgaagcagt ttttgcagaa aataatttag gaatcaatca
     1921 gtcagaaatc agtgcattat tctcatatat aaaagaagga gcgtcaagtt agtgaaaatg
     1981 tcagacgagg ctttaaaaat tatcgaggaa agtcaaaatc gggaaaagtt aaaacaagtg
     2041 attgatctaa ttgaagagcg attagtagaa catgctattg taccaaccga gttgcagtgg
     2101 accattctaa ttaatcactt gaatgagatg ctgaaacgtt ctcaaagaaa ggaaacgatt
     2161 cccgtagtcg atcgggattt gttcaaagaa gtatccaaag aagccataac tatttctgat
     2221 gaaatagtta gaagtatcgg taatttaacc gatgatgaaa tatatgtttt gtcgattcat
     2281 tttgaaacag caaaaaataa tta
//
LOCUS       DARBET010000156         2283 bp    DNA     linear   BCT 07-NOV-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4027
            SAMEA114569841-rid20112183.denovo.162, whole genome shotgun
            sequence.
ACCESSION   DARBET010000156 DARBET010000000
VERSION     DARBET010000156.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA114569841
            Sequence Read Archive: ERR12203666
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2283)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2283)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001972618.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001972618.1
            Genome Coverage       :: 132x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/07/2023 13:18:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,041
            CDSs (total)                      :: 2,975
            Genes (coding)                    :: 2,908
            CDSs (with protein)               :: 2,908
            Genes (RNA)                       :: 66
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 67
            CDSs (without protein)            :: 67
            Pseudo Genes (ambiguous residues) :: 0 of 67
            Pseudo Genes (frameshifted)       :: 26 of 67
            Pseudo Genes (incomplete)         :: 28 of 67
            Pseudo Genes (internal stop)      :: 20 of 67
            Pseudo Genes (multiple problems)  :: 6 of 67
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2283
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA114569841-rid20112183.denovo.162"
                     /isolate="VRE4027"
                     /db_xref="taxon:1352"
                     /country="Denmark"
     gene            <1..750
                     /locus_tag="SCJ86_002845"
     CDS             <1..750
                     /locus_tag="SCJ86_002845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS30 family transposase"
                     /protein_id="HEG1170326.1"
                     /translation="KSNCGRRPLVLPEEQSEYIQRKVVQGWTPDVIVGRAAFPISCSA
                     RTIYRMFKKGLFDSSDLPMKGKRKPNGHQERRGKQTFRRSIHEREKDYSQFSNEFGHL
                     EGDTIVGLKHKSAVITLVERLSKVIITLKPCGRQAIDIEKKLNQWFESVPKNLFKSIT
                     FDCGKEFSNWKQISNVNDIAIYFADPGTPSQRGLNENSNGLLRRDGLLKSMDFNSVDE
                     FFIQSVASKRNNIPRKSLDYRTIIEHLWKYF"
BASE COUNT          829 a          302 c          459 g          693 t
ORIGIN      
        1 aaatcaaact gtggtagacg tccgcttgtt ttacccgagg aacaatcaga atatattcaa
       61 agaaaggttg ttcaaggatg gacacccgat gtgattgttg gtcgtgcagc gtttcctatt
      121 agttgttctg ctcgtaccat ttatcgtatg ttcaaaaagg ggctatttga ttcttctgac
      181 ttaccgatga aaggcaagcg taaaccgaat ggacatcaag aaagacgtgg aaaacaaact
      241 ttccgccgct ctattcatga acgtgaaaag gattatagcc aattctcaaa tgagtttggt
      301 caccttgaag gtgacactat cgtaggtctg aaacataaaa gtgctgtaat taccttagtt
      361 gaacgattat caaaagttat catcacattg aaaccgtgtg gtagacaagc gattgatatt
      421 gaaaaaaaat taaatcaatg gtttgaatct gtaccgaaaa acctattcaa atccatcact
      481 tttgattgtg gaaaggaatt ttcaaattgg aaacagatca gtaatgtcaa tgatattgcc
      541 atttatttcg ctgatccagg aacgccgtct caaagaggcc taaacgagaa ttctaacgga
      601 ttgttacgta gagatggttt attgaaatct atggatttca attcagtaga tgaatttttt
      661 attcaatctg tcgcatctaa acgaaataat attcctagaa aatcactgga ttatcgaacg
      721 attatcgaac acctttggaa gtatttttga gttacgtaag tattgatgat ctgtctaatt
      781 taatttgaca attaagaaag tattaacttg ttttgtgaaa aagagaattt gaaaaacgat
      841 tggcgaacaa atgctcggta ggtatggtat aaatacagag ctaattggtg tgatgaaatc
      901 agatattcgc gtgagattca gtaaagtagg agttcgaaaa cagctaggtg acaaacagta
      961 ttgctttggt acttcggaat ggtttatcaa attcttatca acagttcact tctctttatg
     1021 tttgagtgat gattcgttag gttattgctt ggaaagaccg gatgagagta gaagagttta
     1081 attttataaa gtgaaaagag gtaatgtacg atatacgtag ggctgaacca atagaggata
     1141 agcaaacgat gttacaggcg caaaattcat agcttactgt tttggagata acagaagagg
     1201 aaataatgca gaagtaaggc gaaaaaagga cggaagccta acagcatatg aaataaagaa
     1261 aaatattata actactgata aatcggtatt gagcaaagta ctaataggag tcaaaaacat
     1321 tcataagcta tgagtgtttc ttggctactt ttttattttt atgtatctac aaaagtttcc
     1381 aggggcagaa cctgtgatta ttaatggaag aacctcaagt attgtcttaa atcattgtta
     1441 tgaaaatgga ttaaaagagt tcatcaaggt gtgattaaca agttactttt cattacatcg
     1501 aatttttcag aagttgctta tattggtgat gatattaatg atttatcatg tatgacatct
     1561 gtgaagaagc gggaggaata gtagaatgtc caaaaaatgc cacaagaaat gagattgaat
     1621 tatctgattt tattgtaact cacagtggtg gtgacgaatc tgtgatagag tttattgagt
     1681 ggatattgga atcgatatag tgtagtagta cagaaaaatt atcatacaat taatactagg
     1741 cataagaata ttggcatatt ctagagttaa aagtaactat tcgaaaagga taaggttaga
     1801 attttttgtt ttgccagtgg aagaatataa aaagggcatt ggaaattaat atattagaat
     1861 gctaagcgga gaaaaaaata tatgaaaagt acgtccttgt gatgggatgg aaaaatacac
     1921 ttatttcaaa caacgataat ataattatgt gaaccgaagt atactcgctt ctaattcaat
     1981 cctcaaatcc cgacagtcgc gacttcaaaa aagcgtatag ttttaatgta tccccatatg
     2041 atccgaaaaa tggtaattta caattaacag tttgatgatg ttgaagatgt ggaaatgctg
     2101 aaattaccat gatgagaaac acattaatta aagggaatta attaactaga atataatctg
     2161 attcaatttg aattaagcaa aaaaaggctg agttttaaat aatcgcgaat tgtaaaatta
     2221 agttagaaaa ataaaaaggc atttatggta cactcaaatt gtatttccga cgaaagaaaa
     2281 caa
//
LOCUS       DARBET010000157         2264 bp    DNA     linear   BCT 07-NOV-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4027
            SAMEA114569841-rid20112183.denovo.163, whole genome shotgun
            sequence.
ACCESSION   DARBET010000157 DARBET010000000
VERSION     DARBET010000157.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA114569841
            Sequence Read Archive: ERR12203666
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2264)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2264)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001972618.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001972618.1
            Genome Coverage       :: 132x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/07/2023 13:18:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,041
            CDSs (total)                      :: 2,975
            Genes (coding)                    :: 2,908
            CDSs (with protein)               :: 2,908
            Genes (RNA)                       :: 66
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 67
            CDSs (without protein)            :: 67
            Pseudo Genes (ambiguous residues) :: 0 of 67
            Pseudo Genes (frameshifted)       :: 26 of 67
            Pseudo Genes (incomplete)         :: 28 of 67
            Pseudo Genes (internal stop)      :: 20 of 67
            Pseudo Genes (multiple problems)  :: 6 of 67
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2264
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA114569841-rid20112183.denovo.163"
                     /isolate="VRE4027"
                     /db_xref="taxon:1352"
                     /country="Denmark"
     gene            104..>2264
                     /gene="acm"
                     /locus_tag="SCJ86_002846"
     CDS             104..>2264
                     /gene="acm"
                     /locus_tag="SCJ86_002846"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="collagen-binding MSCRAMM adhesin Acm"
                     /protein_id="HEG1170327.1"
                     /translation="MKKCRNIILSMLFIITNITSLIPVHVYADAGRDISSNVTSLTVD
                     PTNITDGGNIKVKFSFDEKKQNIQPGDYLWINWPSEGNIRGEGFQKEIPLMIENKNVG
                     TLTVRKDSAQVVFNENIKNLDSVEGWGQFEIQARNVTDTGEENTGPFTVTSGDKTATV
                     NVTKPASGSSSSVFYYKTGDMLPEDTKHIRWFLNINNNGTYVEQPVKISDEIQSGQRL
                     DPSTFEINQIHLGEQKVYRGEEGIQQFLQDFPSATFNFSVTDNYIEITIPKNFVNLRK
                     IMVSYKTIIENPEQINFENHSEAWFKEFNKPAVDGESFNHTVKNISASGGVNGTVRGE
                     LKIFKYINDTEIGIPNVTFELRRADEQPIQGQSSILLTSNEQGEASIKGLQVGDYVVK
                     EKEAPNWIDFDPLSSNELKFSINENDTEGVSLPIYNKKKVTNITATKIWNGGTTPRPS
                     IYFKLFRASTNNWEPVPDAETKRLDNGITSVTWEDIQQYDDSGNEYTFKVQEVDQNGN
                     DYVPSGYQKIENGLVVTNENKEVISVSGQKTWEDNENQDGKRPTTITVNLLADGQPIQ
                     HKEVSEKDDWRYRFTNLPKYRDGQEIIYTVTEDNVPEYSTTIDGYNIKNSYTPEKTNI
                     SIPGSHITPWKNFPEKTEREKIRNLFSQKLQIFAHTGESSEQRSNYISKRAVPKQITN
                     KSKSILPKTSSKKSSFAVIIGLLIVTICAGIFLQKYKK"
BASE COUNT          891 a          348 c          448 g          577 t
ORIGIN      
        1 tagttaaaag attggaaatg agtgggggag gaattgaaga aaggattttt gagagatgat
       61 atagtaggtt ttatttttag ttaaaatagg ggagatagaa aaaatgaaaa agtgtagaaa
      121 tattatttta tcaatgttat ttattattac taatataaca tcactgattc ctgttcatgt
      181 ctatgcggat gcaggcagag atatcagcag taatgtcact tcgttgaccg tggacccaac
      241 gaatattaca gatggaggaa acataaaagt aaaattctcg tttgacgaaa aaaaacaaaa
      301 tatccaacca ggtgattatc tttggatcaa ttggccaagt gaaggaaata ttcgcggtga
      361 aggattccaa aaagaaatcc cattaatgat tgaaaataaa aatgttggaa ctttaacggt
      421 cagaaaagat tctgcgcagg ttgtgtttaa tgaaaatatt aaaaacctag actcagtaga
      481 aggttggggg caatttgaga tacaggccag aaacgtaacc gataccggtg aagaaaatac
      541 gggaccattc accgtgacga gcggtgataa aacagctact gtaaatgtga caaagccagc
      601 ttctggttct tcttctagcg ttttttatta taaaacagga gacatgctac ctgaagatac
      661 taagcacatt cgatggtttt tgaatattaa caataacggc acttatgttg aacaaccagt
      721 taaaatcagt gatgaaatcc aaagtggaca aagattagat ccaagtacgt ttgaaattaa
      781 tcaaatacac ttaggtgaac agaaggttta tagaggagaa gagggaatcc aacaattttt
      841 acaagatttc cccagcgcta catttaattt ttcagtaact gataattaca ttgaaataac
      901 tataccaaaa aactttgtca atcttaggaa aataatggtg agttataaga cgatcataga
      961 aaacccagaa caaataaatt ttgagaatca ttcggaagct tggtttaaag aatttaataa
     1021 acctgctgtt gatggagaaa gtttcaatca tacagttaaa aatatttcag cttctggtgg
     1081 agtcaacggt acagttagag gcgaattgaa gattttcaaa tatatcaatg acacagagat
     1141 tggtatccca aatgtgactt tcgaattaag acgagcagat gaacagccta ttcaaggaca
     1201 gtctagtatc ctgttgactt cgaatgaaca gggagaagct agtatcaaag gcctgcaagt
     1261 aggagactat gttgtaaaag aaaaagaagc acccaattgg attgattttg atccactaag
     1321 ttcaaatgaa ctcaaattct ccataaacga aaatgataca gagggagtca gtttacctat
     1381 ttataacaag aaaaaggtta cgaacatcac agcaactaaa atttggaacg gtggaaccac
     1441 tccaagaccg agtatttatt ttaaattatt cagagcaagt acaaacaatt gggaaccagt
     1501 tcctgatgca gaaacaaaac gattagacaa tggaataact tcagtgactt gggaagatat
     1561 ccaacagtat gatgatagtg gaaacgaata tacgtttaaa gtgcaagaag tagatcaaaa
     1621 cggaaatgat tatgtccctt caggttatca aaaaattgaa aacgggctag tcgttacaaa
     1681 tgagaataaa gaagtcattt cagtctcagg acaaaaaaca tgggaagaca atgagaacca
     1741 agacggcaaa cgaccgacga cgatcacagt aaatctatta gccgacggtc aaccgatcca
     1801 acacaaagaa gtaagtgaaa aagatgattg gcgctatcgt ttcacaaatc tgccaaaata
     1861 cagagacggg caagaaatca tctatactgt aacggaagac aacgtgcccg aatacagtac
     1921 gacgattgac gggtataaca tcaaaaacag ttatacaccg gaaaaaacaa atatatctat
     1981 accaggtagc catataacac cgtggaaaaa ttttccagag aagacagaga gagagaaaat
     2041 tagaaatcta ttctctcaaa aattgcaaat atttgctcat acaggagaat ccagcgaaca
     2101 aagaagcaat tatattagta agagagctgt acctaaacag ataacaaata aatcaaaaag
     2161 cattcttcct aaaacaagta gtaaaaaatc tagttttgct gtaatcattg gtttattgat
     2221 agtaactatc tgtgctggga tattcttaca aaaatataaa aaag
//
LOCUS       DARBET010000158         2104 bp    DNA     linear   BCT 07-NOV-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4027
            SAMEA114569841-rid20112183.denovo.164, whole genome shotgun
            sequence.
ACCESSION   DARBET010000158 DARBET010000000
VERSION     DARBET010000158.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA114569841
            Sequence Read Archive: ERR12203666
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2104)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2104)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001972618.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001972618.1
            Genome Coverage       :: 132x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/07/2023 13:18:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,041
            CDSs (total)                      :: 2,975
            Genes (coding)                    :: 2,908
            CDSs (with protein)               :: 2,908
            Genes (RNA)                       :: 66
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 67
            CDSs (without protein)            :: 67
            Pseudo Genes (ambiguous residues) :: 0 of 67
            Pseudo Genes (frameshifted)       :: 26 of 67
            Pseudo Genes (incomplete)         :: 28 of 67
            Pseudo Genes (internal stop)      :: 20 of 67
            Pseudo Genes (multiple problems)  :: 6 of 67
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2104
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA114569841-rid20112183.denovo.164"
                     /isolate="VRE4027"
                     /db_xref="taxon:1352"
                     /country="Denmark"
     gene            169..1539
                     /locus_tag="SCJ86_002847"
     CDS             169..1539
                     /locus_tag="SCJ86_002847"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324486.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4357 domain-containing protein"
                     /protein_id="HEG1170328.1"
                     /translation="MMDKSNEEQTTLYTKYWRRLEEVFDNSKGMEDFFRYYLAAITGE
                     YSAKHILYQAFKDYWHKERDVSSDAELLQKLVRYAGYFARLYYNKPEGKYSEVLSDFQ
                     SMESMMPAPFVLGLSEWYYHDQFITEDQYIDAIKVVNDYQLRRYFNGDDTSRVSKAFP
                     SYLKSVRKYAKANGYENIVDIVIYVLVTRNQSNNMALPSDKALKSNLLNANAYAMRLA
                     RWLLEKIENRENSATLDMSNLSIEHIMPQTSTSYWEEKAGTSGEEYTGLVNTIGNLTL
                     VTNPDNSAAGNKDFETKKKIFEDTLHIRMNKDLYELTEWTSSDISARSEALINELITM
                     YPYLRSSGDYEHDGNREIFLEAQGIKATGYLNEDETVIIHSGSEIYSKIKDIASDSLD
                     ETRQELLDNGIIEETIGGLQFVQDYTASSVSNAAALLLGGSRNGWDYWKYDNGISIND
                     SLRNKK"
     gene            1560..1844
                     /locus_tag="SCJ86_002848"
     CDS             1560..1844
                     /locus_tag="SCJ86_002848"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303603.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEG1170329.1"
                     /translation="MDKNKLRYAILKLRDEKKNPFIELKGQVPEEDILEQTKFLSRNG
                     LLESMVWADNTVHIWGSVSLEGEKYLVENSGLAKAYALAKEVKNWIPFWG"
BASE COUNT          731 a          308 c          444 g          621 t
ORIGIN      
        1 ttgatttacc aacatgttta gacattttcc tctaggaaaa aattaactag caccacgcgt
       61 attgtttata ttaaattaga tgaccgtgat aatgcacagc aaatttttga aagtatcaat
      121 tctaccggtg aaagattgac tgcctcggat ttgattcgaa acttcatcat gatggataaa
      181 tctaatgaag agcagactac gctttatact aaatattggc gtaggttgga agaagtcttt
      241 gataattcta aaggaatgga agactttttc agatactacc ttgctgcaat aactggggag
      301 tattcagcaa agcatattct ttatcaagca ttcaaagact attggcataa agagcgcgat
      361 gtatcaagtg atgccgaatt gctccaaaaa ttagtgcgat atgctggtta ttttgcgaga
      421 ctttactata acaaacctga aggaaaatac tcagaagtat tgagcgattt ccaaagtatg
      481 gaaagtatga tgcctgcacc ttttgttttg ggattatctg aatggtacta tcatgaccaa
      541 tttatcactg aagaccaata tattgatgcg attaaggtag tgaatgatta tcagcttagg
      601 agatatttta atggggatga tacaagcaga gtgtcaaaag catttccttc atatttgaaa
      661 agtgttagga agtacgctaa agctaatgga tatgaaaata tcgttgatat tgtaatttat
      721 gttcttgtta ctagaaatca atcaaataat atggcacttc catctgacaa ggcattgaaa
      781 tctaatctat tgaatgccaa tgcttatgcg atgagattgg cccgttggtt acttgaaaaa
      841 atagagaatc gagaaaattc agcaacactt gatatgagta acttgtccat tgaacacatc
      901 atgccacaga ctagtacttc atattgggaa gagaaggctg gtacatcggg ggaagaatac
      961 acaggattag taaataccat tggtaattta acactcgtta caaatcctga taattcggcc
     1021 gcgggaaata aagactttga gaccaaaaag aaaatctttg aagataccct tcatattcgt
     1081 atgaataaag atttgtacga attgactgag tggacatcat cggatatttc tgccagaagt
     1141 gaagctctaa tcaatgaact gattactatg tatccctatt tgagatcgtc gggcgactat
     1201 gaacacgatg ggaataggga aattttctta gaggcgcaag gaataaaagc aactgggtat
     1261 ttgaacgaag acgagaccgt gattatacat tcaggttcag aaatttattc taagattaaa
     1321 gatattgcat ctgattcgct agatgaaact cgccaagagc tgttggataa tggaatcatt
     1381 gaagaaacaa ttgggggact acaatttgtt caagattaca ctgcctcatc tgtttcgaat
     1441 gcagcagctc tgctattagg gggtagccgg aatggttggg actactggaa atatgataac
     1501 ggaataagta ttaatgattc gctaagaaat aagaaataga gcatagaggt ggtgttaggt
     1561 tggataagaa caaactaaga tatgcaattc ttaaattgcg tgatgaaaaa aagaatcctt
     1621 ttattgagct taaagggcaa gttccagaag aagatatttt agaacaaact aaatttcttt
     1681 cgcgcaatgg cttactagaa tccatggttt gggcggataa cactgttcat atctggggga
     1741 gtgtttctct ggaaggggaa aaatatttag ttgaaaactc aggtcttgct aaggcgtatg
     1801 cattggcaaa agaagttaaa aattggattc cattttgggg ctaagataag tttttaaacg
     1861 gagaaggtag caaatcataa ttggtttgtt gccttttttg attgcccaaa aagaaaaatg
     1921 acaaagtata gagataaatg acatagtaca cgggaaaaat gacattgtat caccgacaac
     1981 gtctaaatag atgttgatat atatacgttc cgtacaaata attataaaaa tttaaatgat
     2041 ttgagtagaa gtatcagtga atatctcaag gggaatagct atctcaagat tcgattttca
     2101 cata
//
LOCUS       DARBET010000159         2065 bp    DNA     linear   BCT 07-NOV-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4027
            SAMEA114569841-rid20112183.denovo.165, whole genome shotgun
            sequence.
ACCESSION   DARBET010000159 DARBET010000000
VERSION     DARBET010000159.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA114569841
            Sequence Read Archive: ERR12203666
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2065)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2065)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001972618.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001972618.1
            Genome Coverage       :: 132x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/07/2023 13:18:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,041
            CDSs (total)                      :: 2,975
            Genes (coding)                    :: 2,908
            CDSs (with protein)               :: 2,908
            Genes (RNA)                       :: 66
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 67
            CDSs (without protein)            :: 67
            Pseudo Genes (ambiguous residues) :: 0 of 67
            Pseudo Genes (frameshifted)       :: 26 of 67
            Pseudo Genes (incomplete)         :: 28 of 67
            Pseudo Genes (internal stop)      :: 20 of 67
            Pseudo Genes (multiple problems)  :: 6 of 67
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2065
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA114569841-rid20112183.denovo.165"
                     /isolate="VRE4027"
                     /db_xref="taxon:1352"
                     /country="Denmark"
     gene            <1..1008
                     /locus_tag="SCJ86_002849"
     CDS             <1..1008
                     /locus_tag="SCJ86_002849"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347035.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEG1170330.1"
                     /translation="LLDAPYYLSKVALGENLIKPESQQPVTNSNYLINTYNIKPMVKG
                     KKYTITLEGTKPATQVFRPFFTRATGDAWEVGDLQPVEGLTDVWSGTFTAAADPHPTS
                     PQVQICQVPSTSLGNCTIKWLKLEEGDTRTPNIEQYKYRGIGMRDSNNPKDYVWDLAP
                     EYVEDNLATDIKISEITGKANNYTDGKVSEINSQLTASINEVDTTAKDAQTKANANAT
                     AIDELDNKIDERINDTATTTLTVTNGNTGSAKLYREGKTVSIYFVALNGRSSGGNDST
                     ILTIPEGYRPPISFEQLVGSIDRSTLNSAQLSIGADGAIKWRRNSSYGSDYTFAITYT
                     I"
     gene            1025..1174
                     /locus_tag="SCJ86_002850"
     CDS             1025..1174
                     /locus_tag="SCJ86_002850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290627.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEG1170331.1"
                     /translation="MKAAYRPIEPYGFEQIIVNDEEHLPEECTEVEPPIPNWKPKFNY
                     WEGNK"
     gene            1171..1617
                     /locus_tag="SCJ86_002851"
     CDS             1171..1617
                     /locus_tag="SCJ86_002851"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334691.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEG1170332.1"
                     /translation="MKNIWKYGRTGGEYAGKVLDDMLVSVPYTDQPPLEGIRADGEPL
                     TIADQMFDPKLNQWIILANALDHNDLNNLKAMYESLENENGDLKQINAKLMLSDVAIK
                     QENTALKEKADSLAQINSKMMLASLQNSKDISEIKEQLNPASKGGE"
     gene            1619..1756
                     /locus_tag="SCJ86_002852"
     CDS             1619..1756
                     /locus_tag="SCJ86_002852"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336488.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="XkdX family protein"
                     /protein_id="HEG1170333.1"
                     /translation="MFSFSDVKMMYDWGCFTNEQVMVFVPLCITEEKADKIISKEESA
                     S"
     gene            1794..>2065
                     /locus_tag="SCJ86_002853"
     CDS             1794..>2065
                     /locus_tag="SCJ86_002853"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341747.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEG1170334.1"
                     /translation="MINLGEWGAIAGSITAIVSLILLVIKPITASFSKITETLSKVSH
                     NLDLLTKDLESSKSDRLMIHEELKKHDERLDTHAEKLVEHTQQIKT"
BASE COUNT          753 a          358 c          425 g          529 t
ORIGIN      
        1 ctacttgatg caccgtatta tttgagtaag gtggctttgg gtgagaatct aattaaacca
       61 gaatcacaac aaccagttac taatagtaac tatcttatta acacctataa cattaaacca
      121 atggtaaaag gtaagaagta taccatcaca cttgaaggaa ctaagccagc aacacaggtt
      181 tttagaccat ttttcacacg agcaacagga gatgcatggg aggttggtga cttacaacca
      241 gtagaagggt taactgatgt ttggtctgga acatttacag cagcagctga cccacaccct
      301 accagcccac aagttcagat ttgtcaggta ccaagcacaa gtttaggaaa ctgtacaatt
      361 aaatggttaa aactagagga aggcgacaca cgaaccccga atattgagca atataaatac
      421 cgaggaatcg gcatgcgaga ctcaaacaat ccaaaagatt acgtctggga tctagcacca
      481 gaatatgtcg aagacaatct ggccacagat attaaaattt ctgaaattac tggtaaagca
      541 aacaattata ccgatgggaa agtatcggag attaattcgc agttgactgc ttcaattaat
      601 gaagtagaca ccacagctaa ggatgctcaa acaaaagcga atgctaatgc gactgctata
      661 gatgaattag acaataagat cgatgaacgc attaatgata cagctactac cacattaaca
      721 gttacaaacg ggaataccgg atcagcaaag ctttatcgtg aaggaaaaac agtttctata
      781 tattttgtgg ctttaaacgg aagaagcagt ggtggaaatg attcaacgat actaacaatt
      841 ccagaaggct atcggccacc aattagtttt gagcaactgg ttggctcgat agaccgttct
      901 actttgaaca gtgctcagtt atctattggt gcagatggag ccattaaatg gcgaagaaac
      961 tcaagttatg gatcggatta tacctttgca attacttaca cgatttagaa aagcgtgaat
     1021 cgatatgaag gcagcatata gaccaattga accttacgga ttcgagcaaa tcattgtgaa
     1081 tgatgaagaa catttaccgg aagaatgcac agaagtcgaa ccaccgattc caaattggaa
     1141 accgaaattc aattactggg agggaaataa atgaaaaaca tttggaaata tggacgtact
     1201 ggcggagagt acgcaggaaa agtattggac gacatgcttg tatccgttcc ttacacagat
     1261 cagccaccgc ttgaaggaat tcgtgctgat ggcgaaccgt taacaatcgc tgatcaaatg
     1321 tttgatccta aattgaatca atggattatt ttagcgaacg cactagatca caacgattta
     1381 aacaatctca aagcaatgta tgagtcgtta gaaaatgaga acggcgattt aaaacagatc
     1441 aatgccaaac tcatgctaag cgatgtagca attaaacagg aaaatactgc attgaaagaa
     1501 aaagcggata gtttagcaca aatcaattca aaaatgatgc ttgcttcgtt acaaaatagc
     1561 aaagacattt cagaaattaa agagcaacta aatccagctt caaagggagg tgagtagtat
     1621 gtttagtttt agcgatgtga aaatgatgta tgattggggc tgtttcacta acgaacaagt
     1681 aatggttttc gttccgttgt gcattactga agaaaaagca gataaaatca ttagcaaaga
     1741 agagagcgca tcttaattga tgtgcttttt attttgattc aaggagttgt cacatgatta
     1801 atttagggga atggggagcg atagcaggat caataaccgc tatcgtttct ttgattttat
     1861 tagtaataaa accaattact gcatctttct cgaagattac tgagactctt tcaaaagtaa
     1921 gtcacaattt agatttgctg actaaagatt tagaatcgag caaatcagat cgattgatga
     1981 ttcatgaaga actaaagaaa cacgatgaaa gattagatac acatgcagaa aaattggtag
     2041 aacacacgca acaaattaaa acttt
//
LOCUS       DARBET010000160         2058 bp    DNA     linear   BCT 07-NOV-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4027
            SAMEA114569841-rid20112183.denovo.166, whole genome shotgun
            sequence.
ACCESSION   DARBET010000160 DARBET010000000
VERSION     DARBET010000160.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA114569841
            Sequence Read Archive: ERR12203666
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2058)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2058)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001972618.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001972618.1
            Genome Coverage       :: 132x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/07/2023 13:18:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,041
            CDSs (total)                      :: 2,975
            Genes (coding)                    :: 2,908
            CDSs (with protein)               :: 2,908
            Genes (RNA)                       :: 66
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 67
            CDSs (without protein)            :: 67
            Pseudo Genes (ambiguous residues) :: 0 of 67
            Pseudo Genes (frameshifted)       :: 26 of 67
            Pseudo Genes (incomplete)         :: 28 of 67
            Pseudo Genes (internal stop)      :: 20 of 67
            Pseudo Genes (multiple problems)  :: 6 of 67
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2058
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA114569841-rid20112183.denovo.166"
                     /isolate="VRE4027"
                     /db_xref="taxon:1352"
                     /country="Denmark"
     gene            complement(<1..337)
                     /locus_tag="SCJ86_002854"
     CDS             complement(<1..337)
                     /locus_tag="SCJ86_002854"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323513.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication protein"
                     /protein_id="HEG1170335.1"
                     /translation="MTSRKTKDERTRNWTFVVYPESAPENWREFLDELHVPWVESPLH
                     DKDVNPDGELKKPHWHVLIMFSGKKTYDQIREITQKLRSPNPQKCANAKGMVRYFAHM
                     DNPEKFQYAK"
     gene            complement(404..559)
                     /locus_tag="SCJ86_002855"
     CDS             complement(404..559)
                     /locus_tag="SCJ86_002855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299939.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HEG1170336.1"
                     /translation="MISNEKKRVVVTLTKEVADNLDKIAKQMGLSKSGLITIWVNANG
                     KENEQKK"
     gene            1176..1397
                     /locus_tag="SCJ86_002856"
     CDS             1176..1397
                     /locus_tag="SCJ86_002856"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299940.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEG1170337.1"
                     /translation="MRVIKSEKFTLAEIMTTSELAQELDYNQAYVLRLAKEHLTEGKE
                     YRSAGRRNYLFSPEALKKLEKVITTKEGE"
     gene            1398..1655
                     /locus_tag="SCJ86_002857"
     CDS             1398..1655
                     /locus_tag="SCJ86_002857"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377879.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEG1170338.1"
                     /translation="MPKETTINVEQLEKLLESYSNDFIELTGGKVEETTDLFDLLLTN
                     ILELNSEEQKEHKGKLIAPSYRKVAKSAVPTGFLTKQPYIF"
     gene            complement(1791..>2058)
                     /locus_tag="SCJ86_002858"
     CDS             complement(1791..>2058)
                     /locus_tag="SCJ86_002858"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323513.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="replication protein"
                     /protein_id="HEG1170339.1"
                     /translation="SDIIAHGGAEIASYLSVTSAERYELIREMMSFVDSKNITEIKDL
                     IDYAMSERFDDWFPLLCDNSAYIIGQYIKSNRHGGSVNSKINKG"
BASE COUNT          678 a          462 c          314 g          604 t
ORIGIN      
        1 ttttagcata ttgaaatttt tctggattat ccatatgagc aaaatatcgc accatacctt
       61 ttgcattagc acatttttgc gggtttggag accgaagctt ttgagttatc tcacgtattt
      121 gatcatacgt ctttttaccg ctaaacatta tcaaaacatg ccaatgaggc tttttcaatt
      181 caccgtcagg attcacatct ttatcatgaa gtggactctc aacccaagga acatgtaact
      241 catctaaaaa ctccctccaa ttttcaggag ccgattcagg atagactaca aacgtccaat
      301 tccttgtgcg ttcatcttta gtttttctac tcgtcatgct ataattacct taattgaaca
      361 acagtctaac agcaaaagca gtgcgccaac actaatgctt ttgctatttt ttttgctcat
      421 tttcctttcc gttagcatta acccaaattg taataagacc agacttacta agacccattt
      481 gtttcgcaat tttatcaaga ttatctgcca cttccttagt aagcgttaca acgactctct
      541 ttttttcatt actgatcata attcgtatcc tccaacgact ttttcttaaa aaccatagta
      601 acaaaaaagt aactaaggtg caacagttta gatactaatt agcgaataat aggccttaaa
      661 cccttggcat tactgatttt gtcaaaaaaa aacgctgaaa cccttgggag agtaaggcta
      721 aacagctatc gaccaatgac caatgaccaa taaggggggt gtcgtagtcc ccccctcctc
      781 cgcccaattc ataaacacca aaaccaaaaa acaaacctca gggctccgcc ccgaaccccg
      841 acctccttct accgaaatat atgcaggcgc cacgcttact atttcgttca gctatcggct
      901 tttgcaatct aacacgaccg tttcgctacc agtcgcctta caggctccat tctcgcttct
      961 cttacgtgtt ttaacgattg cgccgataac taaacgaaat agacgctaaa tcgcttaaaa
     1021 cgcaaaatag acccttttta gagcattgat tttttctact agtcatttcg ataatataaa
     1081 attgaccaca acacagaaaa cttatattat aagcttatgt cgtaagtccg acgtactcac
     1141 gtaatataga cggacgttaa atggaaggag cataaatgag agtgataaaa tcagaaaaat
     1201 tcactttggc cgaaattatg acgaccagcg aacttgcaca agaacttgat tacaaccaag
     1261 cttacgtttt gcgcttagcg aaagaacatc tgactgaagg aaaagaatat cgctctgccg
     1321 gtagaagaaa ctatcttttc agcccagaag cccttaaaaa actagaaaaa gtcataacca
     1381 ccaaagaagg tgagtaaatg cctaaagaaa caactatcaa cgttgaacaa ctagaaaaat
     1441 tgctagaatc atattccaat gattttatcg aattgaccgg cggaaaagtc gaagaaacca
     1501 ccgacctctt tgacctactt ctcacaaata ttctggaatt aaactctgaa gaacaaaaag
     1561 agcataaggg aaaactgata gcaccttcct acaggaaggt ggctaagtcg gctgtgccaa
     1621 ctggttttct tacaaaacaa ccttatattt tttgatatca caaaaaaatc cattttggac
     1681 ctattttcgg tcataatata gtacctactt ttggtcatag tttcgtccgt agttttggtc
     1741 atagtgacta agactttcac gcacaaaaaa agagctgaaa ttaacagctc ttatccttta
     1801 tttatttttg aatttacact tccaccatga cgattcgatt ttatatattg cccgataata
     1861 taagctgaat tatcacaaag aagcggaaac caatcatcaa atctttctga cattgcatag
     1921 tcaattaaat ctttgatttc agtgatattc ttagaatcaa caaacgacat catctctcta
     1981 atcaactcat atcgttctgc actcgttact gacaaataac tagcaatttc tgcaccacca
     2041 tgagcgataa tatcactt
//
LOCUS       DARBET010000161         2055 bp    DNA     linear   BCT 07-NOV-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4027
            SAMEA114569841-rid20112183.denovo.167, whole genome shotgun
            sequence.
ACCESSION   DARBET010000161 DARBET010000000
VERSION     DARBET010000161.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA114569841
            Sequence Read Archive: ERR12203666
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2055)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2055)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001972618.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001972618.1
            Genome Coverage       :: 132x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/07/2023 13:18:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,041
            CDSs (total)                      :: 2,975
            Genes (coding)                    :: 2,908
            CDSs (with protein)               :: 2,908
            Genes (RNA)                       :: 66
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 67
            CDSs (without protein)            :: 67
            Pseudo Genes (ambiguous residues) :: 0 of 67
            Pseudo Genes (frameshifted)       :: 26 of 67
            Pseudo Genes (incomplete)         :: 28 of 67
            Pseudo Genes (internal stop)      :: 20 of 67
            Pseudo Genes (multiple problems)  :: 6 of 67
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2055
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA114569841-rid20112183.denovo.167"
                     /isolate="VRE4027"
                     /db_xref="taxon:1352"
                     /country="Denmark"
     gene            <1..365
                     /locus_tag="SCJ86_002859"
     CDS             <1..365
                     /locus_tag="SCJ86_002859"
                     /inference="COORDINATES: protein motif:HMM:NF024729.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="IS3 family transposase"
                     /protein_id="HEG1170340.1"
                     /translation="CVIQTLNKAKIHYHIPEGMILHTDLGSQYTAREVEQWLKTNKIR
                     HSYSRKGTPYDNAGIESFHASLKKEEVYTTSYSDFEEANRALFSYIEGFYNRNRIHSS
                     IHYLTPQEFEELAKEKMA"
     gene            complement(721..1293)
                     /locus_tag="SCJ86_002860"
     CDS             complement(721..1293)
                     /locus_tag="SCJ86_002860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000170424.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HEG1170341.1"
                     /translation="MTKYGYARVSTASQSTKEQIQQLLENCVEKKNIFSEKFTGTKRD
                     RPKFNQLLAGLKPGDEIVVTKLDRFARNTREALEIIEPLLDDNVTIKVLNLGTIENTS
                     MGRMVTRTLLSVAEMERDMIVERTQEGKMFARKNNPNFREGRPKATITPKKRHAYELL
                     TSGKSYKEVEAITGFSRSTLFRIKKKIEES"
     gene            complement(1309..1914)
                     /locus_tag="SCJ86_002861"
     CDS             complement(1309..1914)
                     /locus_tag="SCJ86_002861"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016176895.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell filamentation protein"
                     /protein_id="HEG1170342.1"
                     /translation="MILENKLGLTNQVELAKVEEKLSKQKAKELYDCGKINEIEVGTF
                     KGLSEIHEFLFSDIYDFAGKIRSVNIAKGNFRFAPVMYLEHSLQHIDQMPQTSFEEII
                     KKYVEMNIAHPFREGNGRSTRIWLDLILKEELQKVVDWNLIDKADYLSAMERSPINDL
                     EIRYLISNALTDKIDDRELYMKGIDVSYFYEGYSEYTIDDL"
BASE COUNT          638 a          425 c          308 g          684 t
ORIGIN      
        1 attgtgtcat tcaaacattg aataaagcaa aaatacatta tcacattcca gaaggaatga
       61 ttctacacac tgacttgggc agccaataca cagcaagaga agtggaacaa tggcttaaaa
      121 ccaacaaaat aaggcattct tatagtagaa aaggaacacc ttatgataat gctggaatcg
      181 aatctttcca tgcctcattg aaaaaagaag aagtctacac gactagctac tcagattttg
      241 aagaagcaaa tcgagcgcta tttagctaca ttgaaggatt ttataaccga aatcgaatcc
      301 atagctcgat tcactatcta accccacagg aatttgaaga attagcaaaa gaaaaaatgg
      361 cttaaccgtc tctactaaag acaatcaaca ccgtagggat atggagataa aatcaataga
      421 gtcaatagat tatttatcga taataacact aaaaaagcca cgttcctaaa cgtgacttac
      481 gctgaacgat atgtgttgtt tggtagacga acaaatcgtt cattataaat atatgatggt
      541 atcctaatat agtaagcgtg gctagcatac ctttaattac tttacctttt acagttacca
      601 tacacatttt ttgagcagaa atcaagtaaa taaaaaaata ttatatttca caacatgcta
      661 atagactcat atctagaacg cattaagtat ttacctgtct ggttcgctga gacttctttt
      721 ttaactttct tcaattttct tctttattct aaaaagcgta ctgcgactga aaccggttat
      781 agcttcaact tccttatacg atttacctga agtaagcagt tcatatgcgt gcctcttctt
      841 cggtgtgatt gttgctttcg gacgtccttc tctgaaatta ggattattct ttcttgcaaa
      901 catttttcct tcttgggtac gttccacaat catatcacgt tccatttctg ctacactcaa
      961 caacgtccgt gtcaccattc gccccatgga cgtattttca atcgttccta gattcaaaac
     1021 cttgatcgtt acgttatcat cgagtaaagg ttcaatgatc tctaatgctt ctctcgtgtt
     1081 acgagcaaat cgatcaagct tcgtaacaac tatttcatct ccaggcttca gaccagccaa
     1141 cagctgattg aattttggcc gatcccgttt tgtacccgta aatttttctg aaaaaatatt
     1201 cttcttctct acgcagttct caagcaactg ttgaatttgt tcttttgtac tttgagaagc
     1261 ggttgagaca cgtgcatatc catatttcgt catttggtcc acctactttt acaaatcatc
     1321 tatcgtatac tcagaatacc cttcatagaa atagctgaca tcgattcctt tcatatataa
     1381 ctcgcgatcg tcaattttat cagtcagagc attggaaatt agatagcgta tttccaaatc
     1441 gtttatggga cttcgctcca ttgcagaaag atagtctgct ttatcgatta agttccaatc
     1501 tacaactttt tgcaattctt cctttaaaat gagatccagc caaattctag tacttcggcc
     1561 atttccttct cgaaatgggt gagcgatatt catctccaca tatttcttga taatctcttc
     1621 aaaagaagtt tgtggcatct gatcaatatg ctgtaacgaa tgttctaaat acataactgg
     1681 tgcaaatcga aaatttcctt ttgcaatatt gaccgatcgg atttttccgg caaaatcgta
     1741 aatatccgaa aatagaaatt catgaatttc agataaacct ttaaatgtac caacttcgat
     1801 ttcatttatt tttccacaat cgtacaattc tttcgctttt tgtttactga gtttttcttc
     1861 aaccttagcc aactcaactt gattggttaa tcccaattta ttctctaaaa tcatgcgaaa
     1921 tctccttaac tttatgacat aagtttatga aacactctca accccattat atcgcgattt
     1981 tgtttatgtg tcaaaaacta ttaagtttct gacccccgat agatttttca agtgtctcta
     2041 ttttgcacag tctag
//
LOCUS       DARBET010000162         2007 bp    DNA     linear   BCT 07-NOV-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4027
            SAMEA114569841-rid20112183.denovo.169, whole genome shotgun
            sequence.
ACCESSION   DARBET010000162 DARBET010000000
VERSION     DARBET010000162.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA114569841
            Sequence Read Archive: ERR12203666
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2007)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2007)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001972618.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001972618.1
            Genome Coverage       :: 132x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/07/2023 13:18:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,041
            CDSs (total)                      :: 2,975
            Genes (coding)                    :: 2,908
            CDSs (with protein)               :: 2,908
            Genes (RNA)                       :: 66
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 67
            CDSs (without protein)            :: 67
            Pseudo Genes (ambiguous residues) :: 0 of 67
            Pseudo Genes (frameshifted)       :: 26 of 67
            Pseudo Genes (incomplete)         :: 28 of 67
            Pseudo Genes (internal stop)      :: 20 of 67
            Pseudo Genes (multiple problems)  :: 6 of 67
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2007
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA114569841-rid20112183.denovo.169"
                     /isolate="VRE4027"
                     /db_xref="taxon:1352"
                     /country="Denmark"
     gene            <1..1872
                     /locus_tag="SCJ86_002862"
     CDS             <1..1872
                     /locus_tag="SCJ86_002862"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334404.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I restriction endonuclease subunit R"
                     /protein_id="HEG1170343.1"
                     /translation="SAENMEEIKKMFPKSTWFGFTGTPIFPENRKQAKGQLARTTHDQ
                     YGEVLHTYTIKNALEDGSVLGFQVEHENTVEPTSLENKIYRKLKEVETYAEYSSEQIN
                     RMIDQMEPVKKESYLDPAVFEADEHIQKVIHKMFRPDNAYTKFDFRNGRPTKSAILTT
                     SSIDMAKKYYRAIKEMTKEPDWLTKEFPNQLIREGRTMEDPDFPRIAITYSLEENSRD
                     SSAQQEEMQKIIEEYNQYYDTSWSLADIERYNGDINNRLARKRAEFKQFGKQIDLAIV
                     VDRLLTGFDAPTIQTLFVDRNLEYAGLIQAFSRTNRTYPDKTKGLIVTFRKPATMEKN
                     VEDATKLYSKAKEESGLIYPSYQESKKRFKQAYGKLKEVSSMESIDEHTPLEIRIEYV
                     KAFQELNRAYESLVTYDDYNDDMVKSPTLNNQIQLLEEQIGVYETVKGSLIEEEPEKE
                     GEDFSTIEFYSENTTKLYDIDSTYIDQLLGTYAANSSDAREEIEKALAKLNKTEGVKE
                     VYRQILNAFDVGTLDSNEDIFVIKRRYFTQASDDLIHVFSNEWFVSEKELHASNLQYL
                     PGEDPIPNMKAIINSKDYEGYKAKHPEAKPFKYPQEMKRAWRKMLDDELIPLENELR"
BASE COUNT          733 a          321 c          412 g          541 t
ORIGIN      
        1 agtgctgaaa atatggaaga aatcaaaaaa atgtttccga aatccacttg gtttggcttt
       61 acaggtacac cgatttttcc tgagaatcgg aaacaagcca aagggcaatt ggcacgtaca
      121 acccatgatc agtatggaga ggtcttgcat acctatacca ttaagaatgc gctagaagat
      181 ggctctgtct taggatttca agtagagcat gaaaacaccg tggagccgac ttcactagag
      241 aataaaatct atcggaaatt aaaagaagta gaaacatatg cagagtattc atcagaacag
      301 atcaatcgta tgattgatca aatggaacct gtcaaaaaag aaagttattt ggacccagct
      361 gtttttgaag cagatgaaca tatccaaaaa gtcattcata aaatgttccg tccagataat
      421 gcctatacaa aatttgattt tcgaaatggc aggccaacga aatctgcaat cttaaccacg
      481 agttccattg acatggccaa aaagtattat cgggccatca aagagatgac gaaagagcct
      541 gattggttaa caaaagagtt tcccaatcag ctcattcgtg aaggacgaac gatggaggat
      601 cctgattttc cacgaatcgc tattacctat tcattagaag aaaatagccg agattcaagt
      661 gcccaacaag aagaaatgca aaaaattatt gaggaatata atcagtatta tgatacttcc
      721 tggtcattag cagacattga gcgttataac ggagacatca ataatcgttt ggcacgaaaa
      781 agagcggaat tcaaacaatt tgggaaacaa atcgatttag cgattgtagt ggatcgtcta
      841 ttaacaggtt ttgacgcccc tacgattcaa accctatttg tggatcgtaa tctagagtat
      901 gcaggattga tccaagcttt ttcacgtacg aatcgtacgt atcctgataa gacaaaagga
      961 ttgattgtga cgtttcgtaa gccagcgaca atggagaaga atgtcgagga tgcgacaaaa
     1021 ctgtattcaa aagcaaaaga agaatcaggt ttgatttatc caagttatca ggaatccaaa
     1081 aaaagattca aacaagcata tggtaagtta aaagaggttt catccatgga atccattgat
     1141 gaacatacgc cacttgaaat ccgtatcgag tacgtcaaag catttcaaga attgaatcgt
     1201 gcttatgaat cgttagtgac ttatgacgat tacaatgatg acatggtgaa atctccaact
     1261 ttgaacaatc aaattcaact tttggaagaa caaattggcg tgtacgagac cgtgaagggt
     1321 tcattaatag aggaagaacc agaaaaagaa ggagaagatt tttcaacgat cgaattttat
     1381 agtgaaaata ccactaagtt gtatgatatt gattcaactt atattgatca attactagga
     1441 acttatgctg cgaatagttc agatgcacga gaagaaattg aaaaagcttt ggctaaattg
     1501 aataaaacag aaggagtcaa agaagtttac cgacaaattt taaatgcatt tgatgtgggg
     1561 actttagata gtaatgaaga tatctttgtc attaaacgtc gctattttac acaagcgagt
     1621 gatgatttga ttcatgtctt ttccaatgag tggtttgttt ctgagaaaga attacatgct
     1681 tctaatctgc aatatctacc aggtgaagat ccaattccta atatgaaagc aattatcaac
     1741 agtaaggatt acgaaggata taaagccaaa catccagaag cgaagccatt taaataccca
     1801 caagaaatga agcgagcatg gcgtaaaatg ttagatgatg agttgattcc acttgagaat
     1861 gaattgaggt aaacgagaag agttaagaaa attgtacgat tcaaagatgg tagtcaagtg
     1921 gaagtgtaga aaatagggat agatgtcttt tatttgttca cattttataa cgaagtattt
     1981 aagacagcta ttgaataatg tttcctc
//
LOCUS       DARBET010000163         2003 bp    DNA     linear   BCT 07-NOV-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4027
            SAMEA114569841-rid20112183.denovo.170, whole genome shotgun
            sequence.
ACCESSION   DARBET010000163 DARBET010000000
VERSION     DARBET010000163.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA114569841
            Sequence Read Archive: ERR12203666
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2003)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2003)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001972618.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001972618.1
            Genome Coverage       :: 132x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/07/2023 13:18:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,041
            CDSs (total)                      :: 2,975
            Genes (coding)                    :: 2,908
            CDSs (with protein)               :: 2,908
            Genes (RNA)                       :: 66
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 67
            CDSs (without protein)            :: 67
            Pseudo Genes (ambiguous residues) :: 0 of 67
            Pseudo Genes (frameshifted)       :: 26 of 67
            Pseudo Genes (incomplete)         :: 28 of 67
            Pseudo Genes (internal stop)      :: 20 of 67
            Pseudo Genes (multiple problems)  :: 6 of 67
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2003
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA114569841-rid20112183.denovo.170"
                     /isolate="VRE4027"
                     /db_xref="taxon:1352"
                     /country="Denmark"
     gene            <1..378
                     /locus_tag="SCJ86_002863"
     CDS             <1..378
                     /locus_tag="SCJ86_002863"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336725.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HEG1170344.1"
                     /translation="KNVSTEDFFNYCRLEESGVTVTSGTDLPLFITDIPESIIRAVFR
                     RFPEGEEVWEKETSFTTTELLKSFTSNSFKVNGLSEYGEIKVGKSASLAVFSEDLCTD
                     DWQKVMAAEVIATYLDGDLVYKK"
     gene            426..1826
                     /gene="yfcC"
                     /locus_tag="SCJ86_002864"
     CDS             426..1826
                     /gene="yfcC"
                     /locus_tag="SCJ86_002864"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298077.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative basic amino acid antiporter YfcC"
                     /protein_id="HEG1170345.1"
                     /translation="METMKRKFTFPHSYTLIFILIILAAILTWIIPSGQFETVVENVA
                     GMEKTTVVPGTYHQIDKADYGQGFSSILMAPAEGIISAVEVVAFVLIVGGAFGIILKT
                     GAIDRGLFTLAKVLGDKGILVIPMSMILFSLGGSTFGMSEEVIPLFAVFISLMFSLGY
                     DSMTAILILFLSTQAGYVGSTINPFNVLIAQGVAGVYGNPLLWFRMLSWIVITGISIV
                     FTMLYASRVKKNPLSSIVYESDLKVKHKFQNFDTEKEFSTQDKLIIAGFIVGLGIMIW
                     GIISKGWYMVEISALFLGLGLFAGGIARFSQKEIAEAFVEGCSDFAYAAVIIGLARGV
                     LVILEGGMIIDTILNSLANLLGGLPKFLFTTILLISQVIVSFFVPSSSGAAALTMPVM
                     APLGDLMNVSRDVVVLSNQFGNGLMNIISPTSGVLLAGLAIAEINFSKWFKVGFKIFA
                     ILTLVSAVLLYIATLF"
     gene            1859..>2003
                     /locus_tag="SCJ86_002865"
     CDS             1859..>2003
                     /locus_tag="SCJ86_002865"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEG1170346.1"
                     /translation="MIKINQLAGMNIHYLFYSLKEFFAYQQKIGISSVELWGGAPHFF
                     IDAF"
BASE COUNT          555 a          339 c          432 g          677 t
ORIGIN      
        1 aaaaatgttt ccacagagga cttctttaat tattgtcgat tagaggaaag tggtgtaaca
       61 gtcactagcg gtacagattt accgctgttt attactgata ttcctgaatc gattataaga
      121 gcagtattcc gtcgttttcc tgaaggagaa gaagtctggg agaaagaaac aagcttcacg
      181 acaaccgagc tgctgaaaag ttttacaagc aacagcttca aagtaaatgg tttatcggag
      241 tacggagaaa taaaagtagg gaaaagcgcc agcttagctg ttttcagcga ggacctctgt
      301 actgatgact ggcaaaaagt gatggctgcg gaagttatcg ctacctatct tgatggtgac
      361 ttagtttaca agaaataatg cttttcgata taacgttata aaacgaatta aagagaggtt
      421 aaaaaatgga aacaatgaaa agaaaattca ccttcccaca tagttataca ttgattttta
      481 tcttaattat tttggctgca attttaactt ggataattcc tagtggtcag tttgagacag
      541 ttgttgaaaa cgtcgcaggt atggaaaaaa caacggttgt tccgggaacc tatcaccaaa
      601 tcgataaggc agattatgga caaggttttt cctctatact aatggcacca gctgaaggga
      661 ttatttcagc tgttgaggtt gttgcttttg ttctcatcgt tggtggtgcc ttcgggatta
      721 tcctaaaaac gggagccatc gatcgagggc tgtttacctt agcaaaagtt ctgggagaca
      781 aggggatctt agttattccg atgtcgatga ttctattcag cttaggagga tcaacctttg
      841 gcatgagcga ggaagtcatt cccttatttg cggtgtttat ttctctgatg ttttctcttg
      901 gctatgattc catgactgcg atcttgattc tatttttaag tacacaagcg ggatatgttg
      961 ggtcgacgat taatcccttc aacgttttaa tcgcacaggg agtagctggc gtttacggaa
     1021 atcccctgct ttggtttcgg atgctatcct ggattgtcat tactgggatt tcaattgttt
     1081 tcaccatgct ttatgctagc agagtcaaaa agaatccact gtcctctatt gtttatgaga
     1141 gtgatttaaa ggttaagcat aagtttcaaa attttgacac tgaaaaagag ttttcaacac
     1201 aagataaact aattatcgcc ggttttatcg taggtttagg aatcatgatt tggggtatca
     1261 tttccaaggg atggtatatg gttgaaattt ctgcactatt cttaggatta ggtctttttg
     1321 ctgggggcat cgctagattt tctcaaaagg aaattgctga agcttttgtc gaaggctgtt
     1381 cagatttcgc ctatgctgct gtaatcattg ggctagctag aggagtcttg gtaatcttgg
     1441 aaggtggaat gattattgac actattctaa acagcttggc taacctttta ggtggtcttc
     1501 caaaattcct gttcactacg attctactca tttctcaagt aattgtttcc ttttttgttc
     1561 catcctcctc aggagctgcg gcattaacga tgccggttat ggcaccattg ggtgatctaa
     1621 tgaatgtaag tcgtgatgtt gttgttctaa gtaaccagtt tggaaatggt ttaatgaata
     1681 ttatctcccc aactagtggt gtattactgg caggattagc catcgcagag attaatttct
     1741 ctaaatggtt taaggttggc ttcaaaatat ttgccatctt aacccttgtt tctgctgttt
     1801 tgttatacat tgctacactc ttttagtctt aatcatccat taataggagg caatagttat
     1861 gattaaaata aatcaattag caggaatgaa tatccactat ttattttatt ctctcaaaga
     1921 atttttcgct tatcaacaga aaattggtat ttcttctgtt gaactttggg gaggtgctcc
     1981 gcattttttc atagatgctt tta
//
LOCUS       DARBET010000164         1994 bp    DNA     linear   BCT 07-NOV-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4027
            SAMEA114569841-rid20112183.denovo.171, whole genome shotgun
            sequence.
ACCESSION   DARBET010000164 DARBET010000000
VERSION     DARBET010000164.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA114569841
            Sequence Read Archive: ERR12203666
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1994)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1994)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001972618.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001972618.1
            Genome Coverage       :: 132x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/07/2023 13:18:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,041
            CDSs (total)                      :: 2,975
            Genes (coding)                    :: 2,908
            CDSs (with protein)               :: 2,908
            Genes (RNA)                       :: 66
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 67
            CDSs (without protein)            :: 67
            Pseudo Genes (ambiguous residues) :: 0 of 67
            Pseudo Genes (frameshifted)       :: 26 of 67
            Pseudo Genes (incomplete)         :: 28 of 67
            Pseudo Genes (internal stop)      :: 20 of 67
            Pseudo Genes (multiple problems)  :: 6 of 67
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1994
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA114569841-rid20112183.denovo.171"
                     /isolate="VRE4027"
                     /db_xref="taxon:1352"
                     /country="Denmark"
     gene            274..840
                     /gene="amaP"
                     /locus_tag="SCJ86_002866"
     CDS             274..840
                     /gene="amaP"
                     /locus_tag="SCJ86_002866"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297459.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alkaline shock response membrane anchor protein
                     AmaP"
                     /protein_id="HEG1170347.1"
                     /translation="MNKGLKIIGVIMSLLLLSVFLAIALINAPYIMPEQLERFRFFTL
                     TNYYMQQYIFWAAVVFAVLAIIILLVVLFYPKSRGTFVMKREDGKLTIDKKAIEGLVR
                     SHLHEEEFIHSPKVRIRSTKNRIHIHVNGDLKRTSSLVGKTGVLMQDIEEEVTKVLGT
                     KETVKVAVTYSGYEEQEDTRDYKHSRVE"
     gene            853..1041
                     /locus_tag="SCJ86_002867"
     CDS             853..1041
                     /locus_tag="SCJ86_002867"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875246.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2273 domain-containing protein"
                     /protein_id="HEG1170348.1"
                     /translation="MKELFSAYKLPIIFGGLGLLLAVLWLTIGFFKTLLIIIMTAIGA
                     GIGFYLKQSGIVDQFFKS"
     gene            1054..1611
                     /locus_tag="SCJ86_002868"
     CDS             1054..1611
                     /locus_tag="SCJ86_002868"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295944.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Asp23/Gls24 family envelope stress response
                     protein"
                     /protein_id="HEG1170349.1"
                     /translation="MENKNMNTTNGSVLANEKGENRMMNGELTYEDKVIQKIIGYALK
                     DVDGLLTLDGGFFSNMAEKLVNTDNVTAGINTEVGKKQVAVDLDVIVEYGKDIEDIYN
                     QIKELISTEVNNMTHLDVIEVNVNVADIKTQEEYQKDQETVQDKVTEAAKSTGQFASR
                     QTDKAKSAVGKGAQKVKENNEPRVQ"
     gene            1643..1885
                     /locus_tag="SCJ86_002869"
     CDS             1643..1885
                     /locus_tag="SCJ86_002869"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GlsB/YeaQ/YmgE family stress response membrane
                     protein"
                     /protein_id="HEG1170350.1"
                     /translation="MGFIWALIVGGIIGAIAGAITNKGSSMGIIANIIAGLVGSAIGQ
                     ALLGTWGPTIAGMAIIPSLIGAIILVLVVSFFARKL"
BASE COUNT          725 a          308 c          406 g          555 t
ORIGIN      
        1 aaataaatag aatcttgtaa taataactga gcataatctt tgcaattttt aatcttgaat
       61 aatatccttt aagtatagcc agcaatcaaa agtaaataga gaataagtaa gcaattgtta
      121 tttcagtatc agactggcaa taaaacgccg ttatgatcca gcaatgtttg gtagcgcttt
      181 atttactgat gctatgctaa ctgttcgcta tctatactgt gattgtcggt agagttattt
      241 acagggtaaa aataagttta taggaggtgg aaaatgaata aggggttaaa aataatcggt
      301 gttatcatga gtttactgct actttcagta tttctagcga ttgcgttgat caatgcgccg
      361 tatattatgc ctgaacaatt ggaacgattt cgcttcttta ctttaacgaa ttactacatg
      421 caacaatata ttttctgggc agctgtagta tttgccgttc ttgctattat catcttgcta
      481 gtcgttcttt tctatccaaa gtcaagagga actttcgtta tgaaacggga agatggcaaa
      541 ctaacaatcg acaaaaaagc gatcgaaggg ttggtacgtt cccatttaca cgaagaagag
      601 ttcattcatt cacctaaagt acggatacgt tcaacgaaaa atcgaatcca tatccatgtc
      661 aacggagact tgaaacgaac ttcttcatta gtagggaaaa caggtgtatt gatgcaagat
      721 atcgaagaag aagtaactaa agtactcgga acaaaagaaa cggtcaaagt ggcagtcaca
      781 tacagcggtt atgaagaaca agaagataca agagactaca agcactctcg agtggaatag
      841 gagggccaaa taatgaaaga actattttca gcatacaagc tccctattat ctttgggggt
      901 cttggactac tgctagctgt tttatggtta acgattggat ttttcaagac attgttgatc
      961 ataatcatga cagcaattgg cgcaggaatc ggtttttatt tgaaacagtc aggaatagtt
     1021 gatcaatttt ttaaatcata ggaggaaaca aagatggaaa acaaaaacat gaatacaaca
     1081 aatggatcag ttttggcaaa tgaaaaagga gaaaaccgca tgatgaatgg tgaattgact
     1141 tatgaagaca aagtcatcca aaaaatcatc ggttatgcat tgaaagatgt agatggtcta
     1201 ttgacattag atggcggatt tttctcaaat atggctgaaa aattagtcaa cacagataac
     1261 gtaacagcag gtatcaatac agaagtaggt aagaaacaag tagctgtgga tctagatgtg
     1321 atcgtagaat acggtaaaga catcgaagat atttacaacc aaatcaaaga attgatcagt
     1381 actgaagtaa acaatatgac acatttagat gttatcgaag tgaatgtgaa tgttgcagac
     1441 atcaaaacac aagaagaata ccaaaaagat caagaaacag tacaagacaa agtaacagaa
     1501 gcagcaaaat ccacaggaca atttgcttca cgccaaacag ataaagcaaa atctgctgta
     1561 ggcaaaggtg cacaaaaagt caaagaaaat aacgaaccac gtgtacaata agaagtaaga
     1621 agtcagttag gaggaattta aaatgggatt tatttgggca ttaatcgtcg gcggtatcat
     1681 tggagcaatt gcaggagcaa tcacgaataa aggatcatca atggggatca tcgcaaacat
     1741 cattgctggt ttagtaggtt cagcaattgg acaagcttta ctaggaactt ggggaccaac
     1801 aattgcagga atggctatca ttccatcatt gatcggtgca atcattttag tattagtcgt
     1861 ttcattcttt gcacgtaaat tataagcaaa ttgaagaaaa acttatgttg aaatgaagaa
     1921 caaatttgag aaccgtagcg acactcgctg cggttctttt ttatatattt tatatgaagt
     1981 attctttatt cctt
//
LOCUS       DARBET010000165         1943 bp    DNA     linear   BCT 07-NOV-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4027
            SAMEA114569841-rid20112183.denovo.172, whole genome shotgun
            sequence.
ACCESSION   DARBET010000165 DARBET010000000
VERSION     DARBET010000165.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA114569841
            Sequence Read Archive: ERR12203666
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1943)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1943)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001972618.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001972618.1
            Genome Coverage       :: 132x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/07/2023 13:18:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,041
            CDSs (total)                      :: 2,975
            Genes (coding)                    :: 2,908
            CDSs (with protein)               :: 2,908
            Genes (RNA)                       :: 66
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 67
            CDSs (without protein)            :: 67
            Pseudo Genes (ambiguous residues) :: 0 of 67
            Pseudo Genes (frameshifted)       :: 26 of 67
            Pseudo Genes (incomplete)         :: 28 of 67
            Pseudo Genes (internal stop)      :: 20 of 67
            Pseudo Genes (multiple problems)  :: 6 of 67
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1943
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA114569841-rid20112183.denovo.172"
                     /isolate="VRE4027"
                     /db_xref="taxon:1352"
                     /country="Denmark"
     gene            complement(<1..302)
                     /gene="tnpA"
                     /locus_tag="SCJ86_002870"
     CDS             complement(<1..302)
                     /gene="tnpA"
                     /locus_tag="SCJ86_002870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287522.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS200/IS605-like element ISEfa4 family
                     transposase"
                     /protein_id="HEG1170351.1"
                     /translation="MELDTNNHSVFLLYYHLVLVTKYRRQVIDDEISDYAKTTFERIS
                     ESYHITLVEWNHDKDHVHIMFKAQPKTELTKFINAYKSASSRLIKRDFPRVKQFLW"
     gene            661..915
                     /locus_tag="SCJ86_002871"
     CDS             661..915
                     /locus_tag="SCJ86_002871"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318299.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AbrB family transcriptional regulator"
                     /protein_id="HEG1170352.1"
                     /translation="MGKELKVRKIGNSVGVILPSSLGLKSGDIIQAKQEDNLFILDTT
                     QIAKEHDRKLIEESFQDFEKGLTVSEIEMVKAFGKYGWSE"
     gene            916..1233
                     /locus_tag="SCJ86_002872"
     CDS             916..1233
                     /locus_tag="SCJ86_002872"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="HEG1170353.1"
                     /translation="MKEYTLVYSKSFQTSLETIIEEWENELALSPASIKRFVGAIQKA
                     LELTKNFPTMHEEVSEIYGMDIPTYRIVIGKSYALFYRIDHEKKEILVGRIFQSKQMK
                     LGF"
BASE COUNT          670 a          299 c          363 g          611 t
ORIGIN      
        1 ttccaaagaa attgtttgac tcttggaaag tcacgtttta tcaatcttga gctggcactt
       61 ttataggcat tgatgaattt tgtcaattct gttttaggtt gagctttgaa cataatatga
      121 acgtggtctt tatcatgatt ccactccact aaagtaatgt gatacgattc tgaaattctt
      181 tcaaaagtag ttttggcata atcagatatt tcatcatcaa tcacttgtct acgatatttc
      241 gttactaaaa caaggtggta ataaagaaga aatactgaat gattatttgt atctaattcc
      301 atgttatatc aactcatttc ttgtatagac tgattgtaac atgggacaaa ataaaaaggc
      361 aatacgcctt agttgtcggt cttcgagtta ccaacggtaa ccctcatacc gaacggcatt
      421 catcacccac ctatagagga tgggcgactt ctgcctaatt ctgttaaatg gatgttaacg
      481 gaagtgtatg gtgagtagtt cactcgtatt ttgatatcgt ttgtatatgt tttcagcgtt
      541 ctaagtggct tattttgtcc aataataata tggagtggaa tcccttaaga aacaccgttg
      601 gcaattgttg ttataacatg ttataattta ttcatcaaaa actaaaagga gtgtgataac
      661 atgggaaaag aactaaaggt tagaaagatc ggcaatagcg tgggagtgat ccttccaagc
      721 tctcttggat taaaaagcgg agacattatc caagccaaac aagaagacaa cttattcata
      781 ttagatacta ctcaaatagc aaaagaacac gatcgcaaat tgattgaaga aagtttccaa
      841 gactttgaga aaggtttaac agtttcagaa attgaaatgg taaaagcttt cggaaaatac
      901 ggctggagtg aatagatgaa agagtacacg ctagtttatt ctaaaagttt tcagaccagc
      961 cttgaaacga taatagaaga atgggaaaat gagcttgctc tttctccagc cagcataaaa
     1021 agatttgtag gtgcgatcca gaaggcttta gaactaacca aaaattttcc aactatgcat
     1081 gaggaagttt ccgaaatcta tggaatggat atcccaacat atcggatcgt gatcggcaaa
     1141 agctatgcct tattttatcg gatcgaccat gaaaaaaaag agatacttgt aggacgtatt
     1201 ttccaatcta aacaaatgaa actaggcttt taacaataat taggaaaaag tcgctcatcc
     1261 tctacaggtg ggtgattaat gccgctcgat atggttgtta accgtcggtt actcgaagat
     1321 tgatatataa gacttagtac ctttttattt tgctccatgt tatggccgaa ggtctaaaga
     1381 tacaatcatt agtctagaga aaaattaaga accttaggaa ctacggggat cgcttggtga
     1441 ataagagaca ctgctattag tgaaaaaata agatatcaag tacgctctat tcccaaaaat
     1501 ttcgtgactt tactcatgag aggttcaagt agtatatttt taatcaaaat tgacgtaact
     1561 tttaataaat actacctaaa taaggtagta tttatttttg tgttttaaaa gaggaagtcc
     1621 aatatatcta tgggaaactt cttaaatatt atgttggtac atgaaaatat acttccctgc
     1681 tagactattt acaacatttt actttgtacg tattcttgtt ttaaaaggaa tgggataaaa
     1741 tataggtaca ataactattt gatgctaaat ataattaatg gaggagagat agtgaacata
     1801 cttaatgaaa aaagatatgt atggtttgct ggtaaaaaat atattaaccc gtggtgctag
     1861 ttaattttaa tatcctaaag aatatctcaa agttccttca aaacgtcgtt gtgctcgact
     1921 taatagaaca ctgtaagcca gta
//
LOCUS       DARBET010000166         1930 bp    DNA     linear   BCT 07-NOV-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4027
            SAMEA114569841-rid20112183.denovo.173, whole genome shotgun
            sequence.
ACCESSION   DARBET010000166 DARBET010000000
VERSION     DARBET010000166.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA114569841
            Sequence Read Archive: ERR12203666
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1930)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1930)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001972618.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001972618.1
            Genome Coverage       :: 132x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/07/2023 13:18:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,041
            CDSs (total)                      :: 2,975
            Genes (coding)                    :: 2,908
            CDSs (with protein)               :: 2,908
            Genes (RNA)                       :: 66
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 67
            CDSs (without protein)            :: 67
            Pseudo Genes (ambiguous residues) :: 0 of 67
            Pseudo Genes (frameshifted)       :: 26 of 67
            Pseudo Genes (incomplete)         :: 28 of 67
            Pseudo Genes (internal stop)      :: 20 of 67
            Pseudo Genes (multiple problems)  :: 6 of 67
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1930
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA114569841-rid20112183.denovo.173"
                     /isolate="VRE4027"
                     /db_xref="taxon:1352"
                     /country="Denmark"
     gene            228..794
                     /locus_tag="SCJ86_002873"
     CDS             228..794
                     /locus_tag="SCJ86_002873"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324998.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HEG1170354.1"
                     /translation="MRRKVYTKDQILKAAYDVVAKEGFKGFTARNIAKKMGISTQPIY
                     LEFKNMEDLKNTLLDSIFEQLKEDVFPIERTGDKIIDLGLNYIYFAKKHHSLYMALYI
                     DEHGDGKKMHDFSYNYFREIVKEDERYNQLSDEYVDALHTGTWVAVTGLASLMSSNIM
                     HPTEEQIIALIKRTIDSILALDNPEIIR"
     gene            939..1026
                     /locus_tag="SCJ86_002874"
     tRNA            939..1026
                     /locus_tag="SCJ86_002874"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:971..973,aa:Ser,seq:cga)
     gene            1360..>1930
                     /locus_tag="SCJ86_002875"
     CDS             1360..>1930
                     /locus_tag="SCJ86_002875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342103.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="linear amide C-N hydrolase"
                     /protein_id="HEG1170355.1"
                     /translation="MCTAITYATKDHYFGRNFDYEMSYNEVVTITPRNYRFDFRKVKN
                     LDKHYAMIGIAAGVSNYPLYYEATNEKGLSMAGLNFPGNADYKELQEGKDNVAPFEFI
                     PWILGQCSTIDEAKELLATINLVNIDFSEKLPLSPLHWLLADKEKSIVIESMKDGLHL
                     YDNPVGVLTNNPPFDYQLFNLNNYRSLSNG"
BASE COUNT          683 a          289 c          368 g          590 t
ORIGIN      
        1 ctataacttg aaatgaaaga tttggacaac tatcatttag gtcaaaacta aaaatgtaag
       61 ggaacccatt gaatttaacc aaattttttt aattttgcaa aaaaaagtta aagaagactg
      121 aaaaaaaatt taaaatataa cgttttgagt aaaatgtttg caaaaaatgc taaaaatagg
      181 gtacaataaa acaacatata ctgcagtaaa gaagtggggg agttttaatg agaagaaaag
      241 tctataccaa ggatcaaatt ctaaaagctg cttatgatgt tgttgcaaag gaagggttta
      301 aaggatttac tgcgagaaat attgctaaaa agatggggat ttcgacacag ccgatttatc
      361 tggaattcaa aaacatggaa gatttaaaaa atacgttgct ggattctatc tttgaacaac
      421 taaaagaaga tgttttccct attgaacgta ctggtgataa aatcattgat ctcggtttga
      481 actatatcta ctttgcaaaa aagcatcata gtctttacat ggcattgtac attgatgaac
      541 atggggacgg aaagaaaatg catgattttt catacaatta tttccgtgaa atagttaaag
      601 aagatgaaag atacaatcaa ttatcggatg aatatgtcga tgctctgcat actggtacat
      661 gggtagcagt gactggctta gcgtcattga tgtcatcaaa catcatgcat ccaacagaag
      721 aacaaatcat tgcactgatc aaacggacaa ttgactcgat tttagcatta gataatcctg
      781 aaataatcag atagtgttct atcagtatga atttgatcgg taagtccgtt ggctgcaaac
      841 tgtcaatgga cttttttgct aaaaaaagcg atttctgtta tttatctatt agaaataggc
      901 ttgacatttc gtgaaaaaag tttaaaaata gtacttacgg aaggttggca gagtggtaat
      961 gcaccggact cgaaatccgg cgaaccggct aataccggcg cgcaggttca aatcctgtac
     1021 cttccttata tcaacgatta gaggttgtga agaagctgtc ctgcatcaag taataagaac
     1081 ggccaaacaa aaatagcttt tcctattttt tcgtttgatt gggcttatta ccgcagatgc
     1141 aagcttttga acaccgtttg ttcggaataa ggggctgtga caagactttt gtcacagccc
     1201 cttattgttg tacttttaac ctacaatatt ttttgattcc ccctgagtac tattttttat
     1261 aaatgctagt aaatcaatct tctttggcct ttttttgata aaaaaaatac tagatgtaag
     1321 ataaaagtgt aaaaacgatt tgttttagga ggggaaacga tgtgtaccgc aatcacttat
     1381 gcaacgaaag accattattt tggaaggaat tttgattatg agatgtctta taatgaagtg
     1441 gtcactatta ctccaagaaa ttatcgtttc gattttagaa aggtaaaaaa tttagataaa
     1501 cattatgcaa tgataggaat agcagctggg gtatccaact atccactcta ttatgaggct
     1561 acaaatgaaa aagggttgag tatggcagga ctgaattttc ctggcaatgc tgattataaa
     1621 gaattacaag aaggaaaaga taacgttgca ccatttgagt ttattccatg gatactaggg
     1681 caatgctcaa ccatagacga agctaaagaa ttattggcta ctatcaatct agtcaatatc
     1741 gattttagtg aaaaactacc cttgtcacct ttacactggt tattagctga taaagaaaaa
     1801 tcgatagtaa ttgaaagtat gaaagatgga cttcatttat atgataatcc tgtcggtgtg
     1861 ttgacaaata atccgccatt tgattatcaa ttatttaatc tgaacaatta tcgctcgcta
     1921 tcaaatggaa
//
LOCUS       DARBET010000167         1887 bp    DNA     linear   BCT 07-NOV-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4027
            SAMEA114569841-rid20112183.denovo.174, whole genome shotgun
            sequence.
ACCESSION   DARBET010000167 DARBET010000000
VERSION     DARBET010000167.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA114569841
            Sequence Read Archive: ERR12203666
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1887)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1887)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001972618.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001972618.1
            Genome Coverage       :: 132x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/07/2023 13:18:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,041
            CDSs (total)                      :: 2,975
            Genes (coding)                    :: 2,908
            CDSs (with protein)               :: 2,908
            Genes (RNA)                       :: 66
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 67
            CDSs (without protein)            :: 67
            Pseudo Genes (ambiguous residues) :: 0 of 67
            Pseudo Genes (frameshifted)       :: 26 of 67
            Pseudo Genes (incomplete)         :: 28 of 67
            Pseudo Genes (internal stop)      :: 20 of 67
            Pseudo Genes (multiple problems)  :: 6 of 67
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1887
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA114569841-rid20112183.denovo.174"
                     /isolate="VRE4027"
                     /db_xref="taxon:1352"
                     /country="Denmark"
     gene            complement(62..280)
                     /locus_tag="SCJ86_002876"
                     /pseudo
     CDS             complement(62..280)
                     /locus_tag="SCJ86_002876"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002391850.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
     gene            complement(546..740)
                     /locus_tag="SCJ86_002877"
     CDS             complement(546..740)
                     /locus_tag="SCJ86_002877"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEG1170356.1"
                     /translation="MMGFFLRFYIIELTGRLTVFFGNFAIKPFAEVTAHYICKWLDCK
                     NKIKKVFHYISSSPCQIRPG"
     gene            complement(840..926)
                     /locus_tag="SCJ86_002878"
                     /pseudo
     CDS             complement(840..926)
                     /locus_tag="SCJ86_002878"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339775.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded DNA-binding protein"
BASE COUNT          656 a          266 c          250 g          715 t
ORIGIN      
        1 taggtatttt tattatatat agatttaaca tatgtcggtc ggttgattga tatagctaaa
       61 gttataactc aaccgaagcc gattcatcta cacgaataaa gttgcgagtg ttctcggcta
      121 attcataaaa attgttatgt gttccatata gcaatactgg aataacttta ttttcttgaa
      181 tctcataagc aactctatac acggtgaccg catacttaaa tccattagac caattacctg
      241 ataaacctcc agttttttgc tcttacttaa atggatcaat ggcaatttca tcacaaacct
      301 atagaggatg agtgacttct gcctaattac gttaaaaaat attgaaagta actctatgta
      361 gcaaaaaata tctagaattt catttcctca aaactgatcg atattgtatc taaaaatata
      421 tactacctaa aataggtagt atatattaaa atatgtgctt ttgttagaaa aatcaataca
      481 taaattatca tatctgtata tgtcatttag gtatttcagt tatgttataa tagttttgct
      541 gtctgctacc ccggcctaat ctggcaaggg gaggaggaga tataatgaaa tacctttttg
      601 attttgtttt tgcaatcaag ccacttgcag atgtagtgtg cagttacctc tgcaaatggc
      661 ttgattgcaa aattgccaaa aaagacagta agcctacccg ttaattctat aatatagaaa
      721 cgtaaaaaaa accccatcag cctgcacgct gatggggttt ggtgatataa tgaaatacct
      781 ctttgttact cataattgta ccataattag tcacgaatgc aaccaataga aaaaactact
      841 tagaacggta gatcatcgct aataacaatg cattgtccac caaatggatt ttgtgaagca
      901 ttaaatacat tgttattaat tgtcgctgct tttaactagg cctctcccct tctattataa
      961 aagaagatat atatcatttc ttaattacct actaccttaa accaaaattt aagtactacc
     1021 taatttaagt attacttaaa caatctaaaa aatagttatc ccaattgtta tgcttttata
     1081 atacgattat ttgtaacaaa atgaatatta tataattaaa cactaccata tttaggtagt
     1141 gtttaattat tagcaaaaca aaaaatattt attctattct cttaacttca actaagttat
     1201 gctgcacacc tttatataaa tcatatgcaa ctcattagct agacaaacta caagcatgta
     1261 gttttatctg ttaattatgt actacctaaa taaagtagtt tataattagc aagtatatat
     1321 atttaaaaat ttaagattaa tttttttatg acttgtacaa atttatctga tattctctta
     1381 ttaagaacta cctaatttgg gtagttctta ttttttacat tttaagtagt atttaattgc
     1441 aatttggtag tattttttta ttatcttatt cttagaacgg tagtacttaa tataaatata
     1501 ggtagtatct aatttaagta ttaatggtag tatctaaatt aacaggtagt atgtaactac
     1561 tttaaagtaa tttttttcag attaacacag tagtatctta ttaaataagt taattttaat
     1621 ggtagtacat aattattata tatatctgac attggtagta cttattgtat tttttagtag
     1681 tatataatct attgttttct agcctaaagc cttgtcttat aagaataata taatatatac
     1741 tactgaaata aggtagtata tattactagt aatggtagta tgttttatat gcataaggta
     1801 gttcttaatt tattttttgt tttgacactg gtagtactta attatcttta attttaaaac
     1861 taaattttat tcatacgttt ttctggt
//
LOCUS       DARBET010000168         1804 bp    DNA     linear   BCT 07-NOV-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4027
            SAMEA114569841-rid20112183.denovo.175, whole genome shotgun
            sequence.
ACCESSION   DARBET010000168 DARBET010000000
VERSION     DARBET010000168.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA114569841
            Sequence Read Archive: ERR12203666
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1804)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1804)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001972618.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001972618.1
            Genome Coverage       :: 132x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/07/2023 13:18:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,041
            CDSs (total)                      :: 2,975
            Genes (coding)                    :: 2,908
            CDSs (with protein)               :: 2,908
            Genes (RNA)                       :: 66
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 67
            CDSs (without protein)            :: 67
            Pseudo Genes (ambiguous residues) :: 0 of 67
            Pseudo Genes (frameshifted)       :: 26 of 67
            Pseudo Genes (incomplete)         :: 28 of 67
            Pseudo Genes (internal stop)      :: 20 of 67
            Pseudo Genes (multiple problems)  :: 6 of 67
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1804
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA114569841-rid20112183.denovo.175"
                     /isolate="VRE4027"
                     /db_xref="taxon:1352"
                     /country="Denmark"
     gene            <1..657
                     /locus_tag="SCJ86_002879"
     CDS             <1..657
                     /locus_tag="SCJ86_002879"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEG1170357.1"
                     /translation="VQRVFKILNKNSIFKKKIDDYFVYCPELLLYDVKNIKKIPKIDE
                     NSKLMKNIQSIFGIEEVFVGDKCIFFDQPLLSDGYGIDETDIFAKVNSIFSGKKETLI
                     KLHPRARTNRYGEKNILNSSLPFEVLLLENDFSSTLLISPISTISFSPSMMFEKTINS
                     IMLAKLILNEYTALSEDKKETLTNIVLFCEHYNRLSEEKILLPENWAELRKLVEYGII
                     "
     gene            644..>1804
                     /locus_tag="SCJ86_002880"
     CDS             644..>1804
                     /locus_tag="SCJ86_002880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177091.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oligosaccharide flippase family protein"
                     /protein_id="HEG1170358.1"
                     /translation="MGLFNTLSKSKLVKSSFWYTIGNFFIKGISFITIPIFVNITTQA
                     EYGLINNFTSFVSIFSILVGLSLNSAINNASFDYKDDINEFMSSVLSLATLSLIVFLL
                     IGNIYFLFSNAFFDMNQLVFNLMLLQSFASFVISYIQSYFTINFNHFKYLILSFISTF
                     LNIAISVALMQTLYFDDKYVGRVVGGTLSFVLIGLVIYLRIMIKGAHFFDKKQWRYAL
                     KISLPLIPHSLANIVLAQFDRIMINSYLGPAAGGIYSYISNIGIILSVIWMSTNNAWV
                     PWFYGEMSKNNEKKVKKVSNYYLVVFTLITMIVMVVSIDLAKFMAPKDYYSGLSLVVP
                     ITLGYFFQFLYSLPVNAEFYEKKTSFIAIGTVASAIVNILLNMIFIPRLGIIA"
BASE COUNT          606 a          228 c          285 g          685 t
ORIGIN      
        1 gttcaaagag tttttaaaat attaaataaa aatagcatct tcaaaaaaaa aattgacgat
       61 tattttgtct attgtccgga attgttactt tatgatgtaa aaaatataaa aaaaattccc
      121 aaaatagatg aaaacagtaa attgatgaaa aacatacaat caatctttgg gatagaagaa
      181 gtttttgtcg gagataaatg tatctttttt gatcaacctt tattatctga tggttatggc
      241 atagatgaaa cagatatttt cgctaaagta aatagtatat tttctggaaa aaaagaaact
      301 ttaattaagc ttcatcctag agcaaggaca aatcgttatg gtgaaaaaaa catattgaat
      361 tccagtttac cgtttgaagt cttgctactt gaaaatgact tctcttcaac acttttgatt
      421 agtcctatat ctacaatttc tttttcacca tctatgatgt ttgaaaagac gatcaactct
      481 attatgttgg ctaaattaat tttaaatgaa tatacagcat tatctgaaga taaaaaagaa
      541 acattgacaa atattgttct tttctgtgaa cattataacc gtctatcaga agaaaaaatt
      601 ttactcccag aaaactgggc agaattaaga aagttggtag agtatgggat tatttaacac
      661 actttccaag tcaaaattag ttaagtccag tttctggtat actataggga atttttttat
      721 taagggaatt tcattcataa ctattcctat ttttgtaaat attactactc aagcagaata
      781 tggtttgatt aataatttca cttcatttgt atcaattttt tcgattcttg ttgggctaag
      841 cctaaattcg gcaattaata atgcaagttt tgactataaa gatgacataa atgaatttat
      901 gtcatcggta ctttctctag ctacactttc gttaattgtg ttcttattaa tagggaacat
      961 atattttttg ttttcaaatg ctttttttga tatgaaccaa cttgtattta atttgatgtt
     1021 attacaaagt tttgctagtt ttgtaattag ttatatccag tcttatttta cgattaactt
     1081 taaccatttt aaatatttga ttttatcttt tatatctact tttttgaata ttgcaatctc
     1141 tgtagctttg atgcagacat tatattttga tgataaatat gttggcagag tagtcggcgg
     1201 aacactgtca tttgtgttga taggtttagt aatttattta agaataatga taaagggtgc
     1261 tcattttttt gataaaaaac agtggagata cgctttaaaa atctctttgc cgctaatccc
     1321 acattcctta gcaaatatag ttttagcaca attcgataga attatgatta attcttattt
     1381 aggtcctgct gcggggggaa tatattccta cattagtaac ataggtatta ttctaagcgt
     1441 aatatggatg tctactaata acgcctgggt tccatggttc tatggtgaga tgagtaaaaa
     1501 taatgaaaaa aaagtgaaga aagttagtaa ctattattta gtagtcttta ctttgattac
     1561 gatgattgta atggtagttt cgattgattt agcaaaattt atggctccga aagactatta
     1621 ttctgggtta tctttagtgg ttccgataac cttaggctat ttctttcagt ttttatatag
     1681 tttaccagtt aatgctgaat tttatgagaa aaaaacatcg tttattgcga ttggtacagt
     1741 agcaagtgct atagttaata tactattaaa catgattttc atacctaggt taggaataat
     1801 tgct
//
LOCUS       DARBET010000169         1789 bp    DNA     linear   BCT 07-NOV-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4027
            SAMEA114569841-rid20112183.denovo.176, whole genome shotgun
            sequence.
ACCESSION   DARBET010000169 DARBET010000000
VERSION     DARBET010000169.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA114569841
            Sequence Read Archive: ERR12203666
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1789)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1789)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001972618.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001972618.1
            Genome Coverage       :: 132x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/07/2023 13:18:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,041
            CDSs (total)                      :: 2,975
            Genes (coding)                    :: 2,908
            CDSs (with protein)               :: 2,908
            Genes (RNA)                       :: 66
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 67
            CDSs (without protein)            :: 67
            Pseudo Genes (ambiguous residues) :: 0 of 67
            Pseudo Genes (frameshifted)       :: 26 of 67
            Pseudo Genes (incomplete)         :: 28 of 67
            Pseudo Genes (internal stop)      :: 20 of 67
            Pseudo Genes (multiple problems)  :: 6 of 67
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1789
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA114569841-rid20112183.denovo.176"
                     /isolate="VRE4027"
                     /db_xref="taxon:1352"
                     /country="Denmark"
     gene            complement(<1..161)
                     /locus_tag="SCJ86_002881"
     CDS             complement(<1..161)
                     /locus_tag="SCJ86_002881"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017370594.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS6 family transposase"
                     /protein_id="HEG1170359.1"
                     /translation="MNHFKGKQFQQDVIIVAVGYYLRYNLSYREVQEILYDRGINVSH
                     TTIYRWVQE"
     gene            370..582
                     /locus_tag="SCJ86_002882"
     CDS             370..582
                     /locus_tag="SCJ86_002882"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014834536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEG1170360.1"
                     /translation="MAVWQIQLKERGAYGLISLSDVLCRKEMPDLFSLDQIIAIIGAA
                     AVVITAVAKASDSILAWLKFKKEQKK"
     gene            744..1262
                     /locus_tag="SCJ86_002883"
     CDS             744..1262
                     /locus_tag="SCJ86_002883"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000357244.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfbU family protein"
                     /protein_id="HEG1170361.1"
                     /translation="MDKFNRLTLINQFEILKALNPNEEKYYAEKIEILTEGYEYHYSE
                     IFENISDPLPEEDSRFVLDILNMYRDITFSKNKLKDINSIDNYYIQFKGFDFNSSTEF
                     KLALYAQFFIEKLNRFQEIVEDSDFNTFNSHKPMRGTYIKFLENYNDLKSTNNYQFGN
                     LSYEMISKVLSL"
     gene            1269..1781
                     /locus_tag="SCJ86_002884"
     CDS             1269..1781
                     /locus_tag="SCJ86_002884"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000774078.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEG1170362.1"
                     /translation="MKLNSINDLFAIIGVIANILFSCLNFRFTQKNKKNKLSIEQMKK
                     DIETDLKNDHKERRKIEEYTSLMEKCKATDAIFSFLRKSDEIITRENIDLENEISKLE
                     SIFSDINSLKYLIVEENSDSFLEFEKYLTDLIDNRNNELMRQNIARFLTKLVSEWKSI
                     ISQNMDKLRY"
BASE COUNT          685 a          233 c          273 g          598 t
ORIGIN      
        1 tattcttgca cccaacgata aatcgtcgta tgagaaacgt taatgccacg atcatataag
       61 atttcttgaa cttcacgata gctaaggtta taacgaagat agtagcccac ggctacaata
      121 atcacatcct gctgaaattg ctttccttta aaatgattca tcgtcattcc tcctgctatc
      181 tttttctatt attctacctt atttgatagt agatttaaaa ctttgcaaca gaaccctaaa
      241 aacactatat attccgaaaa gtgagtatct ccattcataa aataatcaac atatttaaaa
      301 aatctaatta gcttttttag tcaatctaaa gttctttatt ctatgatata atgttactgt
      361 agaaagtttg tggcggtttg gcaaatccaa ttgaaggagc gtggtgccta tgggcttatt
      421 agcttatcgg acgtactctg tagaaaggaa atgcctgatt tgttctcatt ggatcaaatc
      481 attgctatta tcggagcagc agcagtagtt attactgccg tagcgaaagc tagcgattcg
      541 attttagctt ggttaaagtt caaaaaagaa caaaaaaaat agagccgtct caacttttgg
      601 cgaggtagac gagctctaaa gctttattta catgccatcc gctttgaacg gactacactg
      661 ggcatgtgac agcatgccct ttcatttata ttatatctca ttcttttata taatgaaagt
      721 aaataaatca tggaggataa aatatggata aatttaatag attaacatta ataaatcagt
      781 ttgaaatttt aaaagcttta aatcctaacg aagaaaaata ctatgctgaa aaaatagaaa
      841 tactaacaga aggttacgaa tatcactata gtgaaatatt tgaaaatatt tctgatccat
      901 tgccagaaga agattcgcgg tttgttctag acatattaaa tatgtataga gatattactt
      961 ttagtaaaaa taaattaaaa gatataaatt caattgataa ttattatata caatttaaag
     1021 gttttgactt taatagctct accgaattta agttagcact ttatgcacaa ttttttattg
     1081 aaaaattaaa taggtttcaa gaaatagttg aagatagtga ttttaacact tttaatagcc
     1141 ataaaccgat gagaggtact tatataaaat ttctcgaaaa ttataatgat ttaaaatcta
     1201 ccaataatta tcaatttgga aacttgtctt atgagatgat aagtaaagtt ctaagtttgt
     1261 gatgataaat gaaattaaat agtataaatg atttatttgc tattataggt gttattgcta
     1321 acatactttt ttcgtgtttg aattttagat ttactcaaaa gaacaaaaag aacaaactca
     1381 gtatagaaca aatgaaaaaa gacattgaaa ctgatttaaa aaatgaccat aaagaaagaa
     1441 gaaaaattga agaatatact tctttaatgg aaaagtgtaa agcaactgat gcaattttta
     1501 gctttttaag aaaaagtgat gagataatca cacgtgaaaa tatagattta gaaaatgaga
     1561 ttagtaaact tgaatctatc ttttccgata taaattctct taagtattta atcgtcgaag
     1621 aaaattcgga tagcttttta gaatttgaaa aatatctaac agatcttata gataatagaa
     1681 ataatgaatt aatgagacaa aacattgcaa gattccttac taaattagtt tctgaatgga
     1741 aaagtattat ttctcaaaat atggataaat taagatatta aggatgtta
//
LOCUS       DARBET010000170         1728 bp    DNA     linear   BCT 07-NOV-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4027
            SAMEA114569841-rid20112183.denovo.177, whole genome shotgun
            sequence.
ACCESSION   DARBET010000170 DARBET010000000
VERSION     DARBET010000170.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA114569841
            Sequence Read Archive: ERR12203666
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1728)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1728)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001972618.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001972618.1
            Genome Coverage       :: 132x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/07/2023 13:18:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,041
            CDSs (total)                      :: 2,975
            Genes (coding)                    :: 2,908
            CDSs (with protein)               :: 2,908
            Genes (RNA)                       :: 66
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 67
            CDSs (without protein)            :: 67
            Pseudo Genes (ambiguous residues) :: 0 of 67
            Pseudo Genes (frameshifted)       :: 26 of 67
            Pseudo Genes (incomplete)         :: 28 of 67
            Pseudo Genes (internal stop)      :: 20 of 67
            Pseudo Genes (multiple problems)  :: 6 of 67
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1728
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA114569841-rid20112183.denovo.177"
                     /isolate="VRE4027"
                     /db_xref="taxon:1352"
                     /country="Denmark"
     gene            complement(244..465)
                     /locus_tag="SCJ86_002885"
     CDS             complement(244..465)
                     /locus_tag="SCJ86_002885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311006.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEG1170363.1"
                     /translation="MEEEKIFEKRWELASVEQRARYHNLMSSYRNIDWTYKEKKYLLW
                     LCQLDVNTFETFEVILDKIKNSNEKRADL"
     gene            complement(563..808)
                     /locus_tag="SCJ86_002886"
     CDS             complement(563..808)
                     /locus_tag="SCJ86_002886"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008271463.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEG1170364.1"
                     /translation="MDFPEVVKEVQEIGATISDSRSSPMNYQDAHGIKIIDQERNVRK
                     YTTINECVINENLCRAPITKHIKNRSKAKDGRTFTTF"
     gene            1048..1662
                     /locus_tag="SCJ86_002887"
                     /pseudo
     CDS             1048..1662
                     /locus_tag="SCJ86_002887"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337511.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
BASE COUNT          571 a          310 c          259 g          588 t
ORIGIN      
        1 tactatatac ttcttcccag aacttgtccg tctccttcaa attatctcta cctctcgagt
       61 tcattgggtg gtttgcccat ttattaaaca tggaatcacc tttctcatac tcatatattt
      121 atgtcctatt tcaattatat ttatatatgc ttaaataaat ttgtatcctg aaatttattg
      181 taatatgaag ctgtatttta catatagtaa ccaaagagtt aataaaataa aaagagcgaa
      241 ccattataga tcagctcttt tttcattgct gtttttaatc ttatctagaa tcacttcaaa
      301 tgtttcaaag gtatttacat ctaactgaca taaccaaagc aagtacttct tttctttgta
      361 agtccaatca atattacggt aagaggacat tagattatgg taccttgctc tttgttcaac
      421 tgaagccaat tcccagcgtt tttcaaatat tttttcttct tccattattg atccctcgca
      481 agacgctacc tcaaacttaa ataatgggca gcgaaagtct aattccttag atctaagtat
      541 agcaaacaaa tatgaaaaaa acttagaagg tagtaaaagt tcttccgtct tttgctttgc
      601 ttctattctt aatgtgtttc gttatcggtg ccctacaaag attttcatta attacacact
      661 catttattgt tgtgtatttt ctgacatttc tctcttgatc aattatttta attccatggg
      721 catcctgata gttcatagga gaggagcggc tatcagaaat agttgcacct atctcttgaa
      781 cttcttttac aacttctgga aaatccatcc atcctgcttc agctgtcaaa agtaataatc
      841 tataatattt atctttcctt ttcaatctaa gaaatcgtca ccccattctt tttcttctat
      901 tataaactat ttaactttta gaattcaaaa accgtttgtc tatttagagt ttaccctaaa
      961 tagacaaaaa tataacattg aaaaatttag agttctaacg tatgattagg gtgtattata
     1021 aacgttatag aaaagtaggg aataaatttg aaaattgctt atattagagt ttcatccatc
     1081 gatcaaaatg aacaacgtca aattgaagaa atgaaaaagt ttggcgctga aaaaattttt
     1141 atcgaaaaac aatctggagc gacgattagt catcggccga ttttccagga agctttagat
     1201 tttgtcagag agcaagatat ttttattgta gaagccattg atcgacttgg acgcaattat
     1261 gacgagatta tcgattcagt gaattatcta aagaagaaga atgtgcgact tattatcacc
     1321 agtttaccga tcatggctga agcaattgga aatcctctcc tagataaatt catcaaagac
     1381 ttaatcattt aaatctcagc catgattgct gaacaagaac gtactgaatc gaaacgacgc
     1441 caggcacaag gaattaaaat cgctaaagca aacggcgtct ataaaggtcg tcctaagtta
     1501 tacagtgcag acaccaaaga tcctcaacgt cgccttgtct ataggagtat cgttcaggat
     1561 cttgagaatg gggttgctat ctcaaagatt gcaacagact acaatgtgac taggcaaaca
     1621 atttaccgaa taaaaaaaga aatcgaccaa ctaattgtat gatttctttt ttgaatcacg
     1681 taattattaa ttagctttgt catcaataca gacaatcatt ttagattc
//
LOCUS       DARBET010000171         1678 bp    DNA     linear   BCT 07-NOV-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4027
            SAMEA114569841-rid20112183.denovo.178, whole genome shotgun
            sequence.
ACCESSION   DARBET010000171 DARBET010000000
VERSION     DARBET010000171.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA114569841
            Sequence Read Archive: ERR12203666
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1678)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1678)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001972618.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001972618.1
            Genome Coverage       :: 132x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/07/2023 13:18:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,041
            CDSs (total)                      :: 2,975
            Genes (coding)                    :: 2,908
            CDSs (with protein)               :: 2,908
            Genes (RNA)                       :: 66
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 67
            CDSs (without protein)            :: 67
            Pseudo Genes (ambiguous residues) :: 0 of 67
            Pseudo Genes (frameshifted)       :: 26 of 67
            Pseudo Genes (incomplete)         :: 28 of 67
            Pseudo Genes (internal stop)      :: 20 of 67
            Pseudo Genes (multiple problems)  :: 6 of 67
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1678
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA114569841-rid20112183.denovo.178"
                     /isolate="VRE4027"
                     /db_xref="taxon:1352"
                     /country="Denmark"
     gene            <1..221
                     /locus_tag="SCJ86_002888"
     CDS             <1..221
                     /locus_tag="SCJ86_002888"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327994.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="phage holin"
                     /protein_id="HEG1170365.1"
                     /translation="NKTFEVLKWFALVIIPALATFVGLVGKALNWQYTDICVVIITGF
                     GAFLGSVLGVSNRTYKMFSAESEEGGNK"
     gene            218..1237
                     /locus_tag="SCJ86_002889"
     CDS             218..1237
                     /locus_tag="SCJ86_002889"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324905.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan recognition protein"
                     /protein_id="HEG1170366.1"
                     /translation="MKKKITITAMSLLTALFLLPINGFAYTINNEFNLGANEGSSQVA
                     NNQYILLHETANETATGRNEAQYMQRSWTSAYTAYIVGDGGIVYQVGQPGYVQYGAGS
                     YANANSPVQIELQHTHDKATFEKNYKAYVELARDSAMKYGIPLTLDTPYNQPGIKSHL
                     WVTQNIWGDHTDPYGYLSEMGVSKEKLAYDLAHGFTDENPTTSENKPVIDPTRAGAAN
                     PTLSDGTNHSHIDQFGEIENANLHVAGWHIANYKYEYIFIMDYNTGKELARVRADGIY
                     RPDVNQAYNTSGNVGYHVSFNMRNFPNKKVYVMMRATNDPEGNTKGGAQDFHDKRWYL
                     NIPKR"
     gene            1426..1499
                     /locus_tag="SCJ86_002890"
     tRNA            1426..1499
                     /locus_tag="SCJ86_002890"
                     /product="tRNA-Ile"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1460..1462,aa:Ile,seq:cat)
BASE COUNT          538 a          304 c          342 g          494 t
ORIGIN      
        1 ataataaaac gttcgaagta ctaaaatggt tcgcactggt aattattccc gcactagcta
       61 ctttcgtggg gttagttggt aaagcgctca attggcagta cacagatatc tgtgttgtca
      121 tcattactgg ttttggcgcg tttttaggga gtgtgttggg tgtatcaaat cgaacctaca
      181 aaatgttctc ggctgaaagc gaagaaggag gaaacaaatg aaaaagaaaa ttactattac
      241 tgcgatgagc ctgttaacgg ctcttttttt attgccaatt aacggatttg cctatactat
      301 caacaatgaa tttaatttgg gcgcaaatga aggtagctca caagtagcaa ataatcagta
      361 tattttactg catgaaacgg ctaatgaaac agcaacagga cgcaatgaag cgcagtatat
      421 gcaacgttca tggactagcg cttatactgc ttatattgtg ggagacggcg gaattgttta
      481 ccaagtcgga caacctggtt atgtacagta cggtgctggt tcatatgcta atgccaatag
      541 tcctgtgcag attgagttac aacacacaca tgataaagca acttttgaaa aaaactataa
      601 agcatacgtt gaattggcta gagattcagc aatgaaatat ggtattccat taacattaga
      661 cacgccttat aaccaaccag gaatcaaatc acatttatgg gtaactcaaa acatttgggg
      721 cgatcacact gatccgtatg gatacttatc agaaatgggt gtaagtaagg aaaaactggc
      781 ttacgattta gctcatggat ttacagacga aaacccaacg acttctgaga acaagcctgt
      841 cattgatcca acccgagcag gtgcagcaaa tccaacttta tcagatggaa cgaaccattc
      901 tcacattgat cagtttggag aaatcgaaaa tgcgaactta cacgtcgctg gatggcacat
      961 cgctaactat aagtacgagt atattttcat tatggactac aatactggga aagaattagc
     1021 tcgagtaaga gctgatggaa tttatagacc ggatgtaaat caagcttata atacttctgg
     1081 aaatgttggc tatcatgtat cttttaacat gcgtaacttc cctaataaga aagtctatgt
     1141 catgatgcgg gcaacgaatg atccagaggg aaacactaaa ggcggtgcgc aagatttcca
     1201 tgacaaacgt tggtatttaa atattcctaa acgataaaaa tagctcctcg ttgaggagca
     1261 ttacataact atattgacaa ctataaaaat cattcgataa aatagtgatg ttatcgcata
     1321 tcttcactat cacccataat agtcacactc caagctatgc gataacaggt ttgttgccac
     1381 acattctact ggttgattgt ttatggcttt atgtggcaac aaccagtacc cttagctcag
     1441 ttggtcagag cagacggctc ataaccgtcc ggtcgtaggt tcgagtccta cagggtacat
     1501 taacgtagcc atttgaatcg ttctgtgtta gaattttttg aagagtatta tacaagctaa
     1561 agcttttctt cattgccact caaatgagtg gcttttttat gtatcctttt atggattaat
     1621 gaaaggatgt ttcacatagt tatatttctg tatatttgaa aagttttact ttgatttt
//
LOCUS       DARBET010000172         1677 bp    DNA     linear   BCT 07-NOV-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4027
            SAMEA114569841-rid20112183.denovo.179, whole genome shotgun
            sequence.
ACCESSION   DARBET010000172 DARBET010000000
VERSION     DARBET010000172.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA114569841
            Sequence Read Archive: ERR12203666
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1677)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1677)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001972618.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001972618.1
            Genome Coverage       :: 132x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/07/2023 13:18:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,041
            CDSs (total)                      :: 2,975
            Genes (coding)                    :: 2,908
            CDSs (with protein)               :: 2,908
            Genes (RNA)                       :: 66
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 67
            CDSs (without protein)            :: 67
            Pseudo Genes (ambiguous residues) :: 0 of 67
            Pseudo Genes (frameshifted)       :: 26 of 67
            Pseudo Genes (incomplete)         :: 28 of 67
            Pseudo Genes (internal stop)      :: 20 of 67
            Pseudo Genes (multiple problems)  :: 6 of 67
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1677
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA114569841-rid20112183.denovo.179"
                     /isolate="VRE4027"
                     /db_xref="taxon:1352"
                     /country="Denmark"
     gene            29..1381
                     /gene="brnQ"
                     /locus_tag="SCJ86_002891"
     CDS             29..1381
                     /gene="brnQ"
                     /locus_tag="SCJ86_002891"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328038.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid transport system II
                     carrier protein"
                     /protein_id="HEG1170367.1"
                     /translation="MTKKLSFKDYLFIGSMLFGLFFGAGNLIFPVHLGQEAGAATFWA
                     NLGFLVTGIGLPFLGVIAIGVSKSSGVYELAKRINQTYAMVFTILLYLVIGPFFALPR
                     LATTSFEIGLAPFIDPSRQTLYLAIFSIVFFVLAWWFSRKPTKILDYVGKFLNPAFLV
                     LLGALLLLAFLNPLGSVNQAPIQPNYQEHAFFTGFTQGYNTLDALAALAFGIIIVTTI
                     QNMGVTKPAEIAKDTIKSGAISIILMGIIYTLLAYLGAMSLGSFALSENGGITLAQIA
                     DHYLGTYGSILLAFIVILACLKTGIGLITAFAETFTDLFPQRNYAFFVALASILPCLA
                     ANVGLTNIIQFSLPVLMFIYPLAMTLILLVLVGPLFKQRTAVYRMTTYFTLIASIFDG
                     LNACPETIKQTPIVQNLLYVAESYLPFFKLGMGWIVPAVIGFVIGLIWSFAKKEEVAD
                     "
BASE COUNT          466 a          291 c          331 g          589 t
ORIGIN      
        1 ttttaaaaaa accgtaaagg agttatgtat gacaaaaaaa ttatcattta aagactatct
       61 ttttataggc tccatgcttt tcggtctatt ttttggtgct ggaaatctta tctttcccgt
      121 tcatttgggt caagaagcag gcgctgctac cttttgggcg aatctaggtt tcttggttac
      181 cggtatcggt cttcccttct taggggtgat tgccattggt gtatcgaaaa gctcaggtgt
      241 atatgaatta gcaaaacgga taaatcaaac atatgcgatg gtttttacca tcttgcttta
      301 cttagttatc ggaccgtttt ttgctttacc tcgccttgca accacatctt tcgagattgg
      361 tttagcacca tttatcgatc ctagcagaca gacgctatat ctagcaattt tcagtattgt
      421 attttttgtg ctggcttggt ggttttcaag aaaaccgaca aagatattag attatgttgg
      481 aaagttcctg aatcctgcat ttttggtttt attaggtgct ttgcttctcc ttgctttttt
      541 gaatccacta ggttctgtaa atcaagcgcc tattcagcca aattatcaag agcatgcttt
      601 tttcacaggg tttactcaag gatataacac attagatgcg cttgctgctt tagcatttgg
      661 gatcatcatt gtcacaacaa tccaaaacat gggtgtaaca aaacctgctg aaatcgctaa
      721 agacacgatc aaatctggtg cgatcagcat tatattgatg ggaatcattt atactttgct
      781 cgcttattta ggtgctatga gcttaggtag ctttgctcta agtgaaaatg gcgggatcac
      841 gctggctcag attgctgatc attatttagg tacttatggc agtatcttat tagcatttat
      901 cgttatatta gcttgcttga agaccggtat tggtttgatt acagcttttg cagaaacatt
      961 cacagatttg tttccgcaaa gaaattacgc attctttgtt gcacttgcta gtatccttcc
     1021 ttgtttagcg gcaaacgtag gcttgacgaa tatcattcaa ttttcgttgc ctgttttgat
     1081 gttcatctat ccattagcaa tgacactgat tttactagtg ttagttggtc ctttgtttaa
     1141 gcagcgtacg gctgtttatc gaatgactac ttattttaca ttgattgctt cgatttttga
     1201 tgggttgaac gcttgcccag aaactatcaa acaaacacct attgttcaaa atcttttata
     1261 tgttgcggaa agctacttgc cgtttttcaa attggggatg gggtggatcg taccagcagt
     1321 tatcggtttt gttatcggac tgatttggag tttcgctaag aaagaagaag tagcagattg
     1381 ataaaagagg ttgtgaaaga gacgttcagc cttgagcaat aaaaagtaat tttgaaaaat
     1441 ggcttctcac atttttgcat aaatttgact taataccgag aaactggctt ttgaacaccg
     1501 tttattgagc gtctataaac tgacctgagt attagagaaa caaaacggca aagtagctcc
     1561 atatactttg ccgttttgtt ttgaactaat accgaagaga agctgatcaa acaacatttc
     1621 ttttataata tagattttag acatatatat gatataaaaa tagttttcgc aaaaaaa
//
LOCUS       DARBET010000173         1474 bp    DNA     linear   BCT 07-NOV-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4027
            SAMEA114569841-rid20112183.denovo.180, whole genome shotgun
            sequence.
ACCESSION   DARBET010000173 DARBET010000000
VERSION     DARBET010000173.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA114569841
            Sequence Read Archive: ERR12203666
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1474)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1474)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001972618.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001972618.1
            Genome Coverage       :: 132x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/07/2023 13:18:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,041
            CDSs (total)                      :: 2,975
            Genes (coding)                    :: 2,908
            CDSs (with protein)               :: 2,908
            Genes (RNA)                       :: 66
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 67
            CDSs (without protein)            :: 67
            Pseudo Genes (ambiguous residues) :: 0 of 67
            Pseudo Genes (frameshifted)       :: 26 of 67
            Pseudo Genes (incomplete)         :: 28 of 67
            Pseudo Genes (internal stop)      :: 20 of 67
            Pseudo Genes (multiple problems)  :: 6 of 67
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1474
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA114569841-rid20112183.denovo.180"
                     /isolate="VRE4027"
                     /db_xref="taxon:1352"
                     /country="Denmark"
     gene            286..693
                     /locus_tag="SCJ86_002892"
     CDS             286..693
                     /locus_tag="SCJ86_002892"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302104.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEG1170368.1"
                     /translation="MSYRVQLIISEDVESQQFGTNVINKVINPALSINAPLIPTALSF
                     SVTAVVSEIEDTEKIKIVEIEVLNKNEKQIFSTGEVSVSLPPQVNDINFNINARNVLV
                     EEAGEHYAVVKFNGTEIGRQIFDIKVNKPVEKN"
     gene            707..1108
                     /locus_tag="SCJ86_002893"
     CDS             707..1108
                     /locus_tag="SCJ86_002893"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEG1170369.1"
                     /translation="MLDIVPSNTHRSGSLINFAAIFACASVILASPVVVDASSTPKTK
                     NDNQLVITTKNEINTISSNDGKIFDVINTVLKRKAEINIKYDEDLNLYFFVIKTTSEL
                     FSSDYDVLDTLDNVLSDYKYMGKSVVATLGE"
     gene            1110..>1474
                     /locus_tag="SCJ86_002894"
     CDS             1110..>1474
                     /locus_tag="SCJ86_002894"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286473.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEG1170370.1"
                     /translation="MFDFNEYLSISKQIESDDKYNSKESYRRTAVSRAYYSAFKKSDE
                     YLKENYDIIYNGSSGKGSHQTVWNLFSTVKELNTLGIHNSGYRMLEKRKCADYIPNET
                     ITKTDMALMNREAEKIINKL"
BASE COUNT          566 a          180 c          258 g          470 t
ORIGIN      
        1 gaggggaagt acatatagtt gttgatatag ttgctaatat tgttagctaa tttgttagta
       61 aaactgtata cgaatttaga tagtaaaaaa acagtttgtt ccttatatag aggcactttt
      121 actactgatt aaatgtcaga tatgcttaca aatatactta taaatatgct tacaaaatta
      181 gctaatattt ccgttgacaa agctttgtta ctattgttaa actaaattga ctagcaaaag
      241 tgaaacataa atttattaaa taaatgtaaa aattaggaga aatcaatgtc ttatagagta
      301 caattaataa tttcagaaga tgtagaaagt cagcaatttg gcactaatgt tattaataaa
      361 gtaattaatc cagctctttc tattaatgcg cctttaattc ctactgcttt atcattttct
      421 gtaacagctg tggtgtctga gatagaggat acagaaaaga taaaaatagt agaaattgaa
      481 gttttaaaca agaatgaaaa acaaattttt tctacgggtg aagtatcggt aagcttgcct
      541 cctcaagtta acgatataaa ctttaatata aacgccagaa atgtcttggt tgaagaagca
      601 ggagagcatt atgctgttgt taaatttaat ggaactgaga ttggaaggca aatctttgat
      661 atcaaggtca acaaaccagt ggaaaaaaat taaggagacg atacggatgc ttgatatagt
      721 accatcaaat acacatcgga gtggtagctt aataaatttt gctgcgattt ttgcttgcgc
      781 atcggtaatt ctagcatctc cagttgttgt ggatgcaagt tctactccga aaactaaaaa
      841 tgataatcaa cttgtaatta ctacaaaaaa tgagattaac acaatttcaa gtaatgatgg
      901 caaaattttt gatgtgataa atacggtttt aaaaagaaaa gctgaaatca atataaaata
      961 tgatgaagat ttaaacttgt atttctttgt tatcaaaaca acatctgaat tgttttcctc
     1021 agattacgat gttttggata ctctcgataa tgtattatct gactataaat atatggggaa
     1081 atctgtagta gcaactttgg gggaataaaa tgtttgactt taatgaatat ttatctattt
     1141 ccaaacaaat tgaatcagat gataaatata attctaaaga atcttataga agaactgctg
     1201 tttcaagagc atactacagt gcttttaaaa aatctgatga gtatttgaaa gaaaattatg
     1261 atattattta taatggaagt agcggaaaag gaagccacca aaccgtttgg aatttgtttt
     1321 caacagttaa ggaattgaat accttaggaa tacataatag tgggtataga atgttagaga
     1381 aaagaaaatg tgctgactat attcctaatg aaaccattac gaaaacagat atggcgctga
     1441 tgaatcgaga agcagaaaaa ataataaaca aact
//
LOCUS       DARBET010000174         1451 bp    DNA     linear   BCT 07-NOV-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4027
            SAMEA114569841-rid20112183.denovo.181, whole genome shotgun
            sequence.
ACCESSION   DARBET010000174 DARBET010000000
VERSION     DARBET010000174.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA114569841
            Sequence Read Archive: ERR12203666
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1451)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1451)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001972618.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001972618.1
            Genome Coverage       :: 132x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/07/2023 13:18:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,041
            CDSs (total)                      :: 2,975
            Genes (coding)                    :: 2,908
            CDSs (with protein)               :: 2,908
            Genes (RNA)                       :: 66
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 67
            CDSs (without protein)            :: 67
            Pseudo Genes (ambiguous residues) :: 0 of 67
            Pseudo Genes (frameshifted)       :: 26 of 67
            Pseudo Genes (incomplete)         :: 28 of 67
            Pseudo Genes (internal stop)      :: 20 of 67
            Pseudo Genes (multiple problems)  :: 6 of 67
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1451
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA114569841-rid20112183.denovo.181"
                     /isolate="VRE4027"
                     /db_xref="taxon:1352"
                     /country="Denmark"
     gene            complement(<1..625)
                     /locus_tag="SCJ86_002895"
     CDS             complement(<1..625)
                     /locus_tag="SCJ86_002895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344895.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4062 domain-containing protein"
                     /protein_id="HEG1170371.1"
                     /translation="MKTINLIKVFIASPGDIAAKRNEVEETIYRWNIENTDNTKVILM
                     PIRWETNAIPTYINNSSGQSVINKQIVENSDILLAIFGHKLGSPVDNYRSGTIAEIDY
                     FYKNSKGEVGVFFLDEPVPQVLAEEYIEVKKYRDSLEDKGFYGTYSKDNIRRFLTKKV
                     NYLIEKAQNNLESKRFDLDSISYKTPSVINKKVTGTDKDFDDNLDIFD"
     gene            complement(784..1050)
                     /locus_tag="SCJ86_002896"
     CDS             complement(784..1050)
                     /locus_tag="SCJ86_002896"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344896.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HEG1170372.1"
                     /translation="MMIKKLIEEKQKDKDFAEAYDREGRKLETAVALYHAREEAGLTQ
                     AELAQKAHTTQSTIARIERGDNVSFDKLDDIARALGKKLVVEFA"
     gene            complement(1040..1396)
                     /locus_tag="SCJ86_002897"
     CDS             complement(1040..1396)
                     /locus_tag="SCJ86_002897"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339788.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="HEG1170373.1"
                     /translation="MEKISFETFKRPNGHDEFLEWLETLPKKDSAKLLRTIEETEKNG
                     MLVAQRLKWVKKLDTNLYELRSKVGSNIQRAIYFHVVNGRYVITHGFTKKTEKTPISE
                     LKHAKELREEWFTNDD"
BASE COUNT          405 a          279 c          181 g          586 t
ORIGIN      
        1 gatcaaaaat atctaagttg tcatcaaaat ctttatcagt tccggtaact tttttgttaa
       61 ttactgaagg tgtcttgtaa gaaatagaat ctaaatcaaa gcgttttgat tctaaattat
      121 tttgtgcttt ctcaattaaa taattcactt tttttgtgag aaaccttctg atattgtctt
      181 tgctatatgt tccataaaaa cccttatctt ctaaagagtc cctgtacttc ttaacttcta
      241 tatactcttc agccaaaact tgtggaactg gttcatctaa gaaaaaaacc cctacctcgc
      301 ctttgctatt tttataaaag taatcaattt ctgctatagt tcccgatcta taattatcaa
      361 ctggagaacc caacttatgg ccaaaaatcg ctaataatat atcactattt tctacaattt
      421 gtttattaat aacactttgc ccagagctat tatttatata agtaggaatc gcattcgttt
      481 cccatcgaat tggcatcaaa attacttttg tattatctgt attttctata ttccatctat
      541 atatggtttc ttctacttca ttcctctttg ctgctatatc acctggtgat gctataaata
      601 cttttattag atttatggtt ttcatttatt gtacctttct ctttatgtct ctttaaagta
      661 atttttgtat aaaatttatt gacttttaca ctataggaca acttttatct atttagtaca
      721 aaaaggccca ctaaactaat tgattagtta ggagcctatt acaggtatat tttcaactcg
      781 cttttaagca aattctacta ccagcttttt gcctaatgcc ctagcgatat catcaagttt
      841 atcgaaagat acgttatccc ctctctcaat tcgagcaata gtagattgag ttgtatgtgc
      901 tttttgtgca agctccgctt gagttaggcc tgcttcctcc cgtgcatgat aaagagcaac
      961 cgcagtttcg agttttcttc cttcacggtc atatgcttct gcaaaatctt tgtctttttg
     1021 cttttcttca atcaattttt taatcatcat ttgtgaacca ctcctctcta agttcttttg
     1081 catgtttaag ttcacttatt ggtgttttct ccgttttttt agtaaatcca tgggtaatga
     1141 cataccttcc attgaccaca tggaaataga tagctctttg gatgtttgat cctaccttac
     1201 ttcttaattc gtacaaatta gtgtctaatt ttttcaccca tttcaaccgt tgagctacta
     1261 gcatcccatt cttttctgtt tcttctattg tacgtaaaag cttcgcacta tctttttttg
     1321 gtaatgtttc caaccattct aaaaattcat catgaccatt aggtctttta aatgtttcaa
     1381 aagatatttt ttccataccc aattatatca tatattgatt ttatattata gtatataaca
     1441 taaaaattcg a
//
LOCUS       DARBET010000175         1440 bp    DNA     linear   BCT 07-NOV-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4027
            SAMEA114569841-rid20112183.denovo.182, whole genome shotgun
            sequence.
ACCESSION   DARBET010000175 DARBET010000000
VERSION     DARBET010000175.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA114569841
            Sequence Read Archive: ERR12203666
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1440)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1440)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001972618.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001972618.1
            Genome Coverage       :: 132x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/07/2023 13:18:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,041
            CDSs (total)                      :: 2,975
            Genes (coding)                    :: 2,908
            CDSs (with protein)               :: 2,908
            Genes (RNA)                       :: 66
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 67
            CDSs (without protein)            :: 67
            Pseudo Genes (ambiguous residues) :: 0 of 67
            Pseudo Genes (frameshifted)       :: 26 of 67
            Pseudo Genes (incomplete)         :: 28 of 67
            Pseudo Genes (internal stop)      :: 20 of 67
            Pseudo Genes (multiple problems)  :: 6 of 67
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1440
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA114569841-rid20112183.denovo.182"
                     /isolate="VRE4027"
                     /db_xref="taxon:1352"
                     /country="Denmark"
     gene            105..1406
                     /locus_tag="SCJ86_002898"
     CDS             105..1406
                     /locus_tag="SCJ86_002898"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002351973.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ISL3 family transposase"
                     /protein_id="HEG1170374.1"
                     /translation="MPDLHHIKLLLGIKDKNIFITNVESKSIKNIKSLVVSATLSKEI
                     RRCPLCKQMNHEGMIVKNGKKKSLIQLNKCANQLTYLALAKQRYHCRGCHTYFTANTY
                     IVDRNCFIAKQVRYKILEELTEKQAMTTIAKHCGVSWSTVSRTLASLIPMTKVKRNWL
                     PRCLLMDEFRSLKNQVGPYSFSCMDGDTGKLLDILPSRKKKALVSYFMQFERRARLKV
                     KVIVTDMNVSYASLIKECFPKAKLVVDRFHIVKHLIRKFEDIRVRIMKSFDRNDPTQA
                     KYYRQLKALSRLLIKRQDKLVYDKWTKWRNFGWAYLTESEVVDRLLSISDELRIAYFY
                     YQEILQAFHDKEADTFFKLVRTMPNSVPKELHSIKKAFINYESGIRLALELPYSNAKI
                     ENLHTHIKALKRVAYGFRSFRKMKTRIFLLNNLITYESKNI"
BASE COUNT          474 a          288 c          264 g          414 t
ORIGIN      
        1 gatagaaaat cattctatcg gtcccttttg agcatgaaaa aacaaaacat acagaagctc
       61 cgttagaatt gaatcaccac aacacaatca aaggagcgtt ctgtatgcct gatcttcatc
      121 atatcaaact actacttgga atcaaagata aaaacatttt catcacaaat gtggaatcga
      181 agagcattaa gaatatcaag agtcttgttg tttctgcgac gttaagtaaa gaaattcgtc
      241 gctgtccttt gtgtaaacag atgaatcatg aaggcatgat cgttaagaac ggaaagaaaa
      301 aatcgctcat tcaacttaat aaatgtgcga accaactgac ttatcttgct ttagcgaaac
      361 aacgctacca ctgtcgtggc tgccacacct attttacggc gaatacctac attgtcgata
      421 gaaactgctt tatcgctaaa caagtgcgct ataaaatttt agaagagcta actgaaaaac
      481 aagcaatgac gacaattgct aagcattgcg gcgtttcttg gtcgaccgtt tctcggacac
      541 tcgcttctct gatccccatg acaaaagtaa aaagaaattg gttaccgcgt tgtttattaa
      601 tggatgaatt tcgttcactg aaaaatcaag tcggtcccta ctccttttct tgtatggatg
      661 gcgatactgg aaaattactg gatattcttc cttcacggaa gaaaaaggct ctcgtttctt
      721 attttatgca atttgaacga cgcgcacgat tgaaggtaaa ggtcatcgtg acggacatga
      781 atgtttctta tgcctctctg ataaaggaat gttttccaaa ggcaaaactc gttgtggatc
      841 gttttcacat tgtcaaacat ctcattcgta aatttgagga tattcgcgta cgaatcatga
      901 aaagctttga tcgaaacgat ccaacacaag ccaaatatta tcgacaatta aaggcgttgt
      961 ctcggcttct gatcaaacga caggacaagc ttgtttatga caaatggacc aaatggcgca
     1021 attttggttg ggcgtatctc accgaaagtg aagtggtgga tcgtctgctt tcgatctctg
     1081 acgaattacg aatcgcctat ttttactatc aagaaattct tcaagcgttt cacgacaaag
     1141 aggcggacac tttctttaaa cttgtaagaa cgatgcccaa ttccgttcca aaagaactgc
     1201 attctataaa aaaagctttt ataaactatg aatccggcat tcgtttagcc ctcgaattgc
     1261 cttattcaaa tgccaaaata gaaaatctgc atacccacat caaagcattg aaacgagtcg
     1321 cttatggctt tcgaagcttt cggaaaatga agaccagaat tttcttacta aataatctga
     1381 ttacctatga atcaaaaaac atctaaagta gaaaaggaat tctaccttag atgttcttat
//
LOCUS       DARBET010000176         1417 bp    DNA     linear   BCT 07-NOV-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4027
            SAMEA114569841-rid20112183.denovo.183, whole genome shotgun
            sequence.
ACCESSION   DARBET010000176 DARBET010000000
VERSION     DARBET010000176.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA114569841
            Sequence Read Archive: ERR12203666
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1417)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1417)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001972618.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001972618.1
            Genome Coverage       :: 132x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/07/2023 13:18:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,041
            CDSs (total)                      :: 2,975
            Genes (coding)                    :: 2,908
            CDSs (with protein)               :: 2,908
            Genes (RNA)                       :: 66
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 67
            CDSs (without protein)            :: 67
            Pseudo Genes (ambiguous residues) :: 0 of 67
            Pseudo Genes (frameshifted)       :: 26 of 67
            Pseudo Genes (incomplete)         :: 28 of 67
            Pseudo Genes (internal stop)      :: 20 of 67
            Pseudo Genes (multiple problems)  :: 6 of 67
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1417
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA114569841-rid20112183.denovo.183"
                     /isolate="VRE4027"
                     /db_xref="taxon:1352"
                     /country="Denmark"
     gene            complement(150..1337)
                     /locus_tag="SCJ86_002899"
     CDS             complement(150..1337)
                     /locus_tag="SCJ86_002899"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296840.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS256-like element IS16 family transposase"
                     /protein_id="HEG1170375.1"
                     /translation="MTQVHFTFESEEIQAIINESGANDTAKTLMTIMFNQLMEEQRNQ
                     YIQANAYERSEERQSQRNGYYDRSFTTRIGTLELHVPRTRDGKFSPTIFERYQRSEKA
                     LIAAMIEMVISGVSTRKVTKTVELLTDGATVSKSFVSNLMKQLDPFVFEWRNRSLEGS
                     EYPFFMCDALYMKVRENHRIVSKGVYIGIGIDSDGRRTILGFDVQDGESEDNWDTVFQ
                     SFVQRGLFGVKLVISDAHKGLVKAVRKNFLGASWQRCQAHFLRNIFDKLPKKVSSDVK
                     DELKSIFKASELELTRERKEHFLEKYGCDSKLSAACDILENGFEDAIQILSFPENIRR
                     RIRTTNVLERLNEEIRRRERVIRIFPNINSITRIIGTLLMEKDTEWLASPRKYLEFNS
                     NNI"
BASE COUNT          412 a          308 c          249 g          448 t
ORIGIN      
        1 taaaagttca acctcatcaa aagcacaatg ttttgcgttg tgaacctaac tgccatgcct
       61 ttctttctga ggagagaggt caggagcagt agcgcagcga ctcttgatgt ctctcctcag
      121 aaagaagaga ctcgcacagg ttcatggtgc taaatgttat tcgaattaaa ttctaaatat
      181 tttcgtggag aagccagcca ctcagtgtct ttttccatca aaagtgttcc aattatccga
      241 gtgatggaat tgatatttgg aaaaatccga atcactcgtt ctctccggcg aatctcttcg
      301 ttcaaccgtt ccaacacatt ggttgtacga atccttcggc ggatattttc aggaaaagac
      361 aaaatttgaa tagcatcttc aaagccgttt tctaaaatgt cgcaagcagc acttaatttt
      421 gaatcacaac catatttttc caagaagtgc tcttttcttt cacgtgtcaa ttcaagttca
      481 gaggctttaa agatactctt caactcgtct tttacatcag aagaaacttt ctttggcagc
      541 ttatcaaaaa tatttctcaa aaagtgggct tggcatcttt gccagctcgc acctaaaaag
      601 ttcttgcgaa cagccttaac taaccctttg tgtgcatcag aaatgaccag ttttacaccg
      661 aacaaacctc gttgaacgaa cgattgaaaa acggtatccc aattatcttc cgattcgccg
      721 tcttgaacat caaaaccaag aatcgtacgt cgtccatcag aatcaatgcc gataccaata
      781 tatacacctt ttgagacaat acgatgattt tcccttactt tcatatacag ggcatcacac
      841 atgaaaaatg gatattccga gccttctagg ctccggtttc tccactcaaa aacaaatgga
      901 tccaactgtt tcatcagatt tgaaacgaat gatttagaaa cagtcgcacc atccgttagc
      961 agttcaactg ttttagttac tttgcgagtg gagacaccag aaatgaccat ttcaatcatg
     1021 gcagcgatca aagctttttc gcttcgctga tatctttcaa agatagtagg actaaactta
     1081 ccgtctctgg ttcgtggaac atgaagttct aacgtaccaa ttcgagttgt aaacgaacga
     1141 tcgtagtaac catttctttg tgattgccgt tcttctgaac gttcataggc atttgcctga
     1201 atatattgat ttcgttgttc ttccatcagc tgattaaaca tgatagtcat aagtgttttt
     1261 gcggtatcat tcgcaccgct ttcgtttata attgcttgaa tttcttcgct ttcaaaagta
     1321 aaatgtactt gagtcattga tatcagtcct attcgagtat gttttctgca aaaaacattg
     1381 tactcctaac tgagatcaat ggcttttttg atgcttc
//
LOCUS       DARBET010000177         1394 bp    DNA     linear   BCT 07-NOV-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4027
            SAMEA114569841-rid20112183.denovo.184, whole genome shotgun
            sequence.
ACCESSION   DARBET010000177 DARBET010000000
VERSION     DARBET010000177.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA114569841
            Sequence Read Archive: ERR12203666
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1394)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1394)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001972618.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001972618.1
            Genome Coverage       :: 132x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/07/2023 13:18:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,041
            CDSs (total)                      :: 2,975
            Genes (coding)                    :: 2,908
            CDSs (with protein)               :: 2,908
            Genes (RNA)                       :: 66
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 67
            CDSs (without protein)            :: 67
            Pseudo Genes (ambiguous residues) :: 0 of 67
            Pseudo Genes (frameshifted)       :: 26 of 67
            Pseudo Genes (incomplete)         :: 28 of 67
            Pseudo Genes (internal stop)      :: 20 of 67
            Pseudo Genes (multiple problems)  :: 6 of 67
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1394
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA114569841-rid20112183.denovo.184"
                     /isolate="VRE4027"
                     /db_xref="taxon:1352"
                     /country="Denmark"
     gene            33..1372
                     /locus_tag="SCJ86_002900"
     CDS             join(33..474,474..1372)
                     /locus_tag="SCJ86_002900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611105.1"
                     /ribosomal_slippage
                     /note="programmed frameshift; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS3-like element ISEnfa3 family transposase"
                     /protein_id="HEG1170376.1"
                     /translation="MRKYTFVFKKKVVSDYLNNEGGYKYLAHKYQINRTLVRHWVRIY
                     NYHGWEGLVGGGKSYTTKFKLDVIEYMETNGLSIQETAKKFNIGSNRTLSKWIEQYEE
                     GGASSLESQKRGRKISMNSKLNIPKKLKDESLEEEVIRLRAENAYFKKVRNLDSRTRF
                     EQEEIKVKTILQLKKEFKLNLLLSIAQLAKSTYYYWVKKLDKPDKYSKIKQEITAIVK
                     ESRNSYGYRRVTLALKMKGYTINHKTVRKLMSQMGLTCQIRIKRYKSYKGTVGKIAKN
                     VLKRNFSVDTPNKKWVTDVTEFKIKGRKIYLSPILDLFNGEIISYSISTSPTYKLIEE
                     MLQQAIKKKGTEGSLILHSDQGWQYQMPQYQKKLKENNIIQSMSRKGNCLDNSVIENF
                     FGVLKSEFFYREKFRSIEIFQSKLNEYIRWYNNKRIKLKLNGLSPVEYRKQSIK"
BASE COUNT          579 a          181 c          244 g          390 t
ORIGIN      
        1 gactagaaat ctaattaaaa ggggtgcttt ttttgcggaa atatacattt gtttttaaga
       61 aaaaagtagt ttcagactat ttaaataacg aaggcggcta caaatatctt gcacataaat
      121 atcaaataaa tcgtacactg gttagacatt gggtaaggat ttataactat catggttggg
      181 aaggcttggt tggaggtggc aaaagctaca ctacaaaatt taaacttgat gttatagaat
      241 atatggaaac aaatggtctt tctatccaag aaactgctaa aaaatttaat atcggttcaa
      301 atagaactct aagtaaatgg atagagcaat atgaagaagg cggtgcttct tcacttgaga
      361 gccaaaaaag gggcagaaaa attagtatga attccaagct aaacattcct aaaaaactta
      421 aagatgagtc tcttgaagaa gaagttattc gtttaagagc agagaacgca tatttaaaaa
      481 agttagaaac cttgattcaa gaacaagatt tgagcaagaa gaaatcaagg ttaaaacaat
      541 ccttcagctc aaaaaagaat ttaaattgaa tctattactt tcaattgcac aattagcgaa
      601 gtcaacttat tattactggg taaaaaaatt agataagcca gataaatata gcaagataaa
      661 gcaagaaatt acagcgattg taaaagaatc aagaaactct tatggttatc gtagagtcac
      721 tttagcgtta aagatgaaag gatatacaat caatcataaa acagttagaa aattaatgtc
      781 ccaaatggga cttacctgtc aaatccgaat aaagcgatat aaatcttata aaggaacagt
      841 aggaaaaatt gccaagaatg tgttaaaacg aaatttttca gtagatacac ccaataaaaa
      901 atgggtgaca gacgtcactg aattcaagat aaaaggaaga aaaatctatc tatctcctat
      961 acttgattta tttaacggag aaataattag ttatagtata tcaactagtc caacatataa
     1021 actgattgaa gaaatgcttc aacaagcaat taaaaaaaag ggaactgagg gttcactgat
     1081 tctacattca gatcaaggat ggcagtatca gatgccacaa tatcaaaaaa aattaaaaga
     1141 gaataacatt attcaaagca tgtctagaaa aggaaattgc ttagataatt ctgtaataga
     1201 aaattttttt ggtgtgctta aatcagagtt tttttatcga gaaaaattcc gatcaattga
     1261 gatatttcaa agtaaattaa atgagtatat taggtggtat aataacaaaa ggataaaact
     1321 aaagttaaat ggactatctc cagtagaata tcggaaacag tccattaaat agttcaactt
     1381 ttggggttca gttc
//
LOCUS       DARBET010000178         1364 bp    DNA     linear   BCT 07-NOV-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4027
            SAMEA114569841-rid20112183.denovo.185, whole genome shotgun
            sequence.
ACCESSION   DARBET010000178 DARBET010000000
VERSION     DARBET010000178.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA114569841
            Sequence Read Archive: ERR12203666
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1364)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1364)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001972618.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001972618.1
            Genome Coverage       :: 132x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/07/2023 13:18:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,041
            CDSs (total)                      :: 2,975
            Genes (coding)                    :: 2,908
            CDSs (with protein)               :: 2,908
            Genes (RNA)                       :: 66
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 67
            CDSs (without protein)            :: 67
            Pseudo Genes (ambiguous residues) :: 0 of 67
            Pseudo Genes (frameshifted)       :: 26 of 67
            Pseudo Genes (incomplete)         :: 28 of 67
            Pseudo Genes (internal stop)      :: 20 of 67
            Pseudo Genes (multiple problems)  :: 6 of 67
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1364
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA114569841-rid20112183.denovo.185"
                     /isolate="VRE4027"
                     /db_xref="taxon:1352"
                     /country="Denmark"
     gene            complement(140..1288)
                     /locus_tag="SCJ86_002901"
     CDS             complement(140..1288)
                     /locus_tag="SCJ86_002901"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HEG1170377.1"
                     /translation="MEQLKAYKFRIYPTEEQEIFFAKSFGCVRKVYNLMLDDRKKAYE
                     EVKNDSSKKMTFPTPAKYKKEFPFLKEIDSLALANAQLNLDKAYKNFFRDKSVGFPRF
                     KSKKNPVQSYTTNNQNGTVALIDSKFIKVPKLKSLVRIKLHRQPKGIIKSATISRHSS
                     GKYYISLLCKEEIIELPKTNSTIGIDLGIMDFAILSDGQKIDNNKFTSKMEKKLKREQ
                     RKLSRRALLAKNKGTNLFEAKNYQKQKRKVARLHEKVMNQRTDFLNKLSTEMIKNHDI
                     ICIEDLNTKGMLRNHKLAKSISDVSWSKFVTKLQYKADWYGRKIIKVDKWFPSSQICS
                     ECGHKDGKKSLEIREWTCPVCHTHHDRDINASINILTEGLRLHSMGLA"
BASE COUNT          392 a          242 c          217 g          513 t
ORIGIN      
        1 taaacctgac ggttcttaac gcttgaagtg ggagcttctt gggaatagag catacttgat
       61 agcgtatttc tttactaaca gcggtgtctc ttatttacca agctatcccc gtagttccta
      121 cggttcttga tgattggatc taagccaatc ccatagaatg tagtcttaga ccttcggtca
      181 gaatattgat actagcattg atatcacgat catgatgagt atgacaaaca ggacaagtcc
      241 attctcgaat ttcaagagat ttcttgccat ctttatgtcc gcactctgaa caaatctgac
      301 tagacggaaa ccatttatct actttgatga tttttcgtcc ataccagtca gccttgtatt
      361 gtaatttagt cacaaacttt gaccaagaaa catcagaaat acttttagct aatttatgat
      421 ttcgcaacat accttttgtg tttaagtctt caatacagat aatatcgtga tttttgatca
      481 tttctgtact caacttattc agaaaatcag tacgttgatt cattactttt tcatgcaatc
      541 tagctacttt gcgtttttgt ttttgataat ttttagcttc aaatagattg gtacctttat
      601 ttttagccaa taaagcacgc ctagacaatt tgcgttgttc acgctttagt ttcttttcca
      661 ttttggacgt gaatttatta ttatcaattt tttgtccatc agaaagaatc gcaaagtcca
      721 taatacctaa gtcaatacca attgtggaat tagttttagg taattcgata atttcttctt
      781 tacacaacaa agaaatatag tatttaccgc tagaatgacg tgatattgta gcggatttga
      841 taatcccttt tggttgtcta tgaagcttaa ttcttactaa tgacttcaat ttaggaactt
      901 tgatgaattt actatcaatc aaagcaactg taccattttg attattcgtt gtataactct
      961 gaacaggatt tttcttactt ttgaaacgtg gaaatccaac ggatttatcc cgaaaaaaat
     1021 tcttgtatgc tttatctaaa ttaagttggg cattggctaa agcaaggcta tcaatttctt
     1081 tcaaaaacgg aaattctttc ttgtatttag ctggtgtcgg aaaagtcatt tttttagaag
     1141 aatcattttt aacttcttca tacgcctttt ttcggtcatc aagcattaga ttgtagacct
     1201 tacggacaca accaaaagat ttggcaaaga agatttcttg ttcctctgtt ggatagattc
     1261 tgaatttgta tgcttttagt tgttccataa agttcaccta ctttctattt atgattgttt
     1321 ccttgatttt ggatatactt cttaatcaca tcaattggtg cgcc
//
LOCUS       DARBET010000179         1355 bp    DNA     linear   BCT 07-NOV-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4027
            SAMEA114569841-rid20112183.denovo.186, whole genome shotgun
            sequence.
ACCESSION   DARBET010000179 DARBET010000000
VERSION     DARBET010000179.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA114569841
            Sequence Read Archive: ERR12203666
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1355)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1355)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001972618.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001972618.1
            Genome Coverage       :: 132x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/07/2023 13:18:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,041
            CDSs (total)                      :: 2,975
            Genes (coding)                    :: 2,908
            CDSs (with protein)               :: 2,908
            Genes (RNA)                       :: 66
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 67
            CDSs (without protein)            :: 67
            Pseudo Genes (ambiguous residues) :: 0 of 67
            Pseudo Genes (frameshifted)       :: 26 of 67
            Pseudo Genes (incomplete)         :: 28 of 67
            Pseudo Genes (internal stop)      :: 20 of 67
            Pseudo Genes (multiple problems)  :: 6 of 67
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1355
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA114569841-rid20112183.denovo.186"
                     /isolate="VRE4027"
                     /db_xref="taxon:1352"
                     /country="Denmark"
     gene            <1..1355
                     /locus_tag="SCJ86_002902"
     rRNA            <1..1355
                     /locus_tag="SCJ86_002902"
                     /product="23S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF02541"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF02541"
BASE COUNT          362 a          295 c          415 g          283 t
ORIGIN      
        1 caagctgtga tggggaggga aataatagta ccgaagttcc tgatgtcaca ctgccgagaa
       61 aagcttctag tgagaaaaca gcggcccgta ccgcaaaccg acacaggtag tcgaggagag
      121 aatcctaagg tgagcgagag aactctcgtt aaggaactcg gcaaaatgac cccgtaactt
      181 cgggagaagg ggtgctgatc atacgatcag ccgcagtgaa taggcccaag cgactgttta
      241 tcaaaaacac aggtctctgc aaaatcgtaa gatgaagtat aggggctgac gcctgcccgg
      301 tgctggaagg ttaagaggag tgcttagcgc aagcgaaggt acgaattgaa gccccagtaa
      361 acggcggccg taactataac ggtcctaagg tagcgaaatt ccttgtcggg taagttccga
      421 cccgcacgaa aggcgtaacg atttgggcac tgtctcaacg agagactcgg tgaaatttta
      481 gtacctgtga agatgcaggt tacccgcgac aggacggaaa gaccccatgg agctttactg
      541 tagtttgata ttgagtgtct gtaccgcatg tacaggatag gtaggagccg tagaaatcgg
      601 aacgctagtt tcgatggagg cgctggtggg atactacccc tgcgttatgg ccactctaac
      661 ccgcaccact aatcgtggtg ggagacagtg tcagatgggc agtttgactg gggcggtcgc
      721 ctcctaaaag gtaacggagg cgcccaaagg ttccctcaga atggttggaa atcattcgaa
      781 gagtgtaaag gcagaaggga gcttgactgc gagaccaaca agtcgagcag ggacgaaagt
      841 cgggcttagt gatccggtgg ttccgcatgg aagggccatc gctcaacgga taaaagctac
      901 cctggggata acaggcttat ctcccccaag agtccacatc gacggggagg tttggcacct
      961 cgatgtcggc tcgtcgcatc ctggggctgt agtcggtccc aagggttggg ctgttcgccc
     1021 attaaagcgg cacgcgagct gggttcagaa cgtcgtgaga cagttcggtc cctatccgtc
     1081 gcgggcgttg gaaatttgag aggagctgtc cttagtacga gaggaccggg atggacttac
     1141 cgctggtgta ccagttgttc tgccaagggc attgctgggt agctatgtag ggaagggata
     1201 aacgctgaaa gcatctaagt gtgaagccca cctcaagatg agatttccca tttctttaag
     1261 aaagtaagat ccctgagaga tgatcaggta gataggtcag gagtggaagt acagtgatgt
     1321 atggagcgga ctgatactaa tcgatcgagg actta
//
LOCUS       DARBET010000180         1324 bp    DNA     linear   BCT 07-NOV-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4027
            SAMEA114569841-rid20112183.denovo.187, whole genome shotgun
            sequence.
ACCESSION   DARBET010000180 DARBET010000000
VERSION     DARBET010000180.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA114569841
            Sequence Read Archive: ERR12203666
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1324)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1324)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001972618.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001972618.1
            Genome Coverage       :: 132x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/07/2023 13:18:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,041
            CDSs (total)                      :: 2,975
            Genes (coding)                    :: 2,908
            CDSs (with protein)               :: 2,908
            Genes (RNA)                       :: 66
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 67
            CDSs (without protein)            :: 67
            Pseudo Genes (ambiguous residues) :: 0 of 67
            Pseudo Genes (frameshifted)       :: 26 of 67
            Pseudo Genes (incomplete)         :: 28 of 67
            Pseudo Genes (internal stop)      :: 20 of 67
            Pseudo Genes (multiple problems)  :: 6 of 67
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1324
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA114569841-rid20112183.denovo.187"
                     /isolate="VRE4027"
                     /db_xref="taxon:1352"
                     /country="Denmark"
     gene            278..820
                     /locus_tag="SCJ86_002903"
     CDS             278..820
                     /locus_tag="SCJ86_002903"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298088.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEG1170378.1"
                     /translation="MLLKVAIKCYCVKNKGGIIYEKIVPRGACLLLALCLSLSAVGCQ
                     KSNENNKIKEGQSISSEEEGMSINANGKNETFKPSDYTLEAKKEYVYEYLGLKFKLSD
                     KFRNYIADKKIAMLDDQSPIDKELKYAILTFEKMTEEQKNAVIEKMGDGYKNWQNELE
                     RIGTIGIFEKNTSEEKNLKL"
     gene            976..>1324
                     /locus_tag="SCJ86_002904"
     CDS             976..>1324
                     /locus_tag="SCJ86_002904"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330914.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TlpA family protein disulfide reductase"
                     /protein_id="HEG1170379.1"
                     /translation="MLSEKTDLENTEAFNKESVKDLRKLPTKDINGKDFTSKDFEKYD
                     LTMVNVFATWCTACVKEIPDLVEVQNEMKSKGVNIVGVVTDPVDDNGENKEAIEKSKL
                     IHEKTKASYPFLMP"
BASE COUNT          533 a          157 c          248 g          386 t
ORIGIN      
        1 catgaaaaat cctccagtgt gttttttatt attctacaca ccttattttt tctgtctagt
       61 ctagtgtagc cgattcaaat tgatgaagca gatcccaaag ggctgcttca tcaatttttt
      121 cgtaagtgtc cgtacactta ctgtgcttgc tcatatatta atcgatcata tactaatcgt
      181 gtaagaacaa ctttaagcat aatacttata ttgttatacc atagtttgga aaaataaaaa
      241 attatatttc atcaacgttt cataaaaggt gtgtagaatg ttattgaaag tagctataaa
      301 atgctattgt gtaaaaaata aaggaggaat aatttatgaa aaaatagtac caagaggagc
      361 atgcctatta cttgcattat gtttgagctt aagcgcagtt gggtgtcaaa aaagtaatga
      421 aaacaataaa ataaaagaag gacaatccat aagttctgaa gaggaaggca tgtcaattaa
      481 tgcaaatggc aaaaatgaaa cttttaagcc atcagattat acattggagg caaagaaaga
      541 atatgtttat gaatatttag ggcttaaatt taaactctca gacaaattta gaaattatat
      601 agctgataaa aagatagcga tgttagatga tcaaagtcct atagataaag aactaaagta
      661 tgctattttg acatttgaga aaatgacaga agaacaaaag aatgcagtta tagaaaagat
      721 gggcgatgga tataaaaatt ggcaaaatga gcttgaaaga attgggacta ttggtatatt
      781 tgaaaaaaat acatcagaag aaaaaaatct aaaattataa aatgtgatac tcatactaaa
      841 ataggagttt caagtgatgg aaaatacgat tgctatttaa gcacaaatag cggagctgaa
      901 agtaatcttc tggatgaatt aaaaagaact gagattcaaa ttatagataa aaaagaacgt
      961 cctaaaaatg gttttgtgct atcagaaaaa actgatttag aaaatacaga agcatttaac
     1021 aaagaatcgg ttaaagattt acgtaaacta ccaacaaagg acataaatgg caaggatttt
     1081 acaagtaaag attttgagaa gtatgatctt actatggtaa atgtatttgc aacttggtgt
     1141 acagcttgtg taaaggaaat tcctgatttg gttgaggttc agaatgaaat gaaaagcaag
     1201 ggtgtaaata tcgttggagt tgttacagat cctgttgatg ataacggtga aaacaaggaa
     1261 gcaattgaaa agtcaaagtt aatacatgaa aagacaaagg cttcatatcc tttcttaatg
     1321 ccag
//
LOCUS       DARBET010000181         1302 bp    DNA     linear   BCT 07-NOV-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4027
            SAMEA114569841-rid20112183.denovo.188, whole genome shotgun
            sequence.
ACCESSION   DARBET010000181 DARBET010000000
VERSION     DARBET010000181.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA114569841
            Sequence Read Archive: ERR12203666
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1302)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1302)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001972618.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001972618.1
            Genome Coverage       :: 132x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/07/2023 13:18:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,041
            CDSs (total)                      :: 2,975
            Genes (coding)                    :: 2,908
            CDSs (with protein)               :: 2,908
            Genes (RNA)                       :: 66
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 67
            CDSs (without protein)            :: 67
            Pseudo Genes (ambiguous residues) :: 0 of 67
            Pseudo Genes (frameshifted)       :: 26 of 67
            Pseudo Genes (incomplete)         :: 28 of 67
            Pseudo Genes (internal stop)      :: 20 of 67
            Pseudo Genes (multiple problems)  :: 6 of 67
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1302
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA114569841-rid20112183.denovo.188"
                     /isolate="VRE4027"
                     /db_xref="taxon:1352"
                     /country="Denmark"
     gene            7..624
                     /locus_tag="SCJ86_002905"
     CDS             7..624
                     /locus_tag="SCJ86_002905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEG1170380.1"
                     /translation="MGFQVLFGSLILTIVFLKWKTSNKKSMWSLVGIVLIIFTVTAAT
                     EANYSHKIQSYKGRLVMTKKDVTRYTSSRTGGLYDYAPLDALKYKDHLKKHEVKVGDR
                     WENASHKAYNSKIVYTVEDADEQNITLPVFNYWGTVVKVNGKKSTIENDNGLVSIKGT
                     GKRTIVEVSSTYPTTMILSLLISSITFIFLFTRYLIRCCGEMKID"
BASE COUNT          490 a          167 c          205 g          440 t
ORIGIN      
        1 agaaccttgg gttttcaagt gctttttggt tcattaatct taaccatagt gtttttaaag
       61 tggaaaacaa gcaacaaaaa aagtatgtgg agccttgtag gaatagttct tattattttt
      121 actgtgactg cagcaacaga agcaaattat tcgcataaaa ttcagtcgta taaaggtcgt
      181 ttagttatga ccaaaaaaga tgttacacgt tatacatcct caagaactgg aggcctatat
      241 gactatgcac cattagatgc attgaaatat aaagatcatt taaagaaaca tgaagtaaaa
      301 gttggtgatc ggtgggagaa tgcatctcat aaagcatata attccaaaat agtctataca
      361 gtagaagatg ccgatgagca aaatatcaca ttacctgtat tcaattattg gggaacagtt
      421 gtgaaagtaa atgggaaaaa gagcactata gaaaatgaca atggtttagt gtctattaaa
      481 ggtacgggga aaagaactat agttgaggtt tcatctacgt atccaactac gatgattcta
      541 tctttattaa ttagttcaat aacttttata ttcttattta cacgttattt aataagatgc
      601 tgcggtgaaa tgaaaataga ttagatagat attataaata tgttgattct attttcatag
      661 caatggagaa aatacattag caatatataa aagaaggtat caagagtcac agtgaattta
      721 ttccgaaaag tgaaaagagc aaatataaaa tttaatgaaa atatagaact aaataggaaa
      781 acgtagacgt aattttctac gttttccttt tcatatatat taaatattac ggaaaattat
      841 ataaactaat ttagtgtttt gtttgtaatt cctaattttg ctccactttt agataatttt
      901 taggtctatt taactcatca gacattctat gctcattttt ctccaaattt gtttcaactc
      961 tttcgtactt aattttgcgt atttgaaaat tcttctttta gagtactttt attacgatac
     1021 tagtatgata tatatctata gcaaacagtt aaaatcttcc taaaataagc gaatagcatt
     1081 cctaaaacct ataaaatttt tagatgaagc gacaagtgga caattaataa actacttgaa
     1141 catgaaatta tggatcacct ttcaaattta gaaaatggaa ttcttatact tattgcgcaa
     1201 catttattga ttgcaaaatt atgtgacaaa aaaaactagt agaagaaaga ataatggctt
     1261 gataataaag tactacctat taaaggtagt actttattta at
//
LOCUS       DARBET010000182         1294 bp    DNA     linear   BCT 07-NOV-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4027
            SAMEA114569841-rid20112183.denovo.189, whole genome shotgun
            sequence.
ACCESSION   DARBET010000182 DARBET010000000
VERSION     DARBET010000182.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA114569841
            Sequence Read Archive: ERR12203666
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1294)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1294)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001972618.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001972618.1
            Genome Coverage       :: 132x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/07/2023 13:18:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,041
            CDSs (total)                      :: 2,975
            Genes (coding)                    :: 2,908
            CDSs (with protein)               :: 2,908
            Genes (RNA)                       :: 66
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 67
            CDSs (without protein)            :: 67
            Pseudo Genes (ambiguous residues) :: 0 of 67
            Pseudo Genes (frameshifted)       :: 26 of 67
            Pseudo Genes (incomplete)         :: 28 of 67
            Pseudo Genes (internal stop)      :: 20 of 67
            Pseudo Genes (multiple problems)  :: 6 of 67
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1294
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA114569841-rid20112183.denovo.189"
                     /isolate="VRE4027"
                     /db_xref="taxon:1352"
                     /country="Denmark"
     gene            <1..1244
                     /locus_tag="SCJ86_002906"
     CDS             <1..1244
                     /locus_tag="SCJ86_002906"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303202.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="IS66 family transposase"
                     /protein_id="HEG1170381.1"
                     /translation="ITEKEHLLPLEEQFCEHCGSQMKDIGRTKVREEIRFHQAMLDCL
                     THYQHTYCCKSCEKEGLSSFKKAIVPKPLISNSLGSNSLVAETIRMKFGQKVPAYRQE
                     NYWKQTHGLDISRDNITNWHIKAVQNALDPLGERLRVYLNQEEILHGDETSYRVIESA
                     KTDTYYWQFCTGKDSQHPIVYYHHDESRAGDVPKTFLKEFTGYLHCDGYSGYNAVESV
                     RLVYCFAHVRRKFFEAIPKGKKNTDIPAAQAVKQLDKWFVLEKKWKDFSPEKRLSCRQ
                     QELRPLFIAFYEWMATIDPVAKSKLDAAVQYACKLRSGFEPIFEDGRLELTNNRAERN
                     IKELVIGRKNWLHSTSLEGARTSGIILSVYKTAELNGLNPVKYLEFLFDKIPNLPVLS
                     AETLDQLLPWNKDVQQHFSRN"
BASE COUNT          423 a          233 c          277 g          361 t
ORIGIN      
        1 ctataacaga gaaagaacat ctccttcctt tggaagaaca attttgtgaa cattgcggtt
       61 cacagatgaa agatatcgga cggacaaaag tacgagaaga aattcgtttc catcaggcta
      121 tgttggattg tctcacccat tatcaacata cctattgttg taaaagctgc gaaaaagaag
      181 gactttcttc ttttaaaaaa gcgatcgttc ctaaaccttt gatttctaat agcttaggtt
      241 ccaatagttt agtggcggaa acgatccgga tgaaatttgg tcagaaagtt cctgcctatc
      301 ggcaagagaa ctattggaaa caaacacatg ggttggatat ttcgagagat aatatcacca
      361 attggcatat taaagcagtg caaaacgcgt tagacccttt aggagaacga ttaagagtct
      421 atcttaatca agaagagatt ctacacgggg atgaaacaag ttatcgtgtg attgaaagtg
      481 ccaaaacaga tacctattac tggcagtttt gtacaggaaa agacagtcag catcccatcg
      541 tttattatca ccacgacgaa agtcgtgctg gagatgttcc gaaaactttt ctcaaggagt
      601 ttacagggta tttacactgt gacggctata gcgggtataa tgccgtggaa agtgtacgac
      661 ttgtctattg tttcgcgcat gttcggcgga aatttttcga agcaattcca aaaggaaaga
      721 aaaacacgga tattcccgca gctcaagcag tcaaacaatt ggataaatgg tttgtcctag
      781 aaaagaaatg gaaagacttt tctcctgaga agcgattaag ctgtcggcaa caagaactgc
      841 gtccattatt tattgctttt tatgagtgga tggcaacgat agatcctgtt gcaaaatcca
      901 agttggatgc agccgttcaa tatgcgtgta aactacgaag tggttttgaa cctatttttg
      961 aagatggtcg tttagaactc acaaataatc gtgcggagcg aaatatcaaa gagctggtga
     1021 taggacggaa aaattggttg cattccacaa gtcttgaagg agcacgaact tcaggaatca
     1081 ttctgagtgt atataaaaca gcagaactta acggattgaa tcctgtaaag tatctagagt
     1141 ttctatttga taagatacca aatcttcccg tactttcagc agaaacttta gatcaactgt
     1201 tgccttggaa caaggatgta caacaacatt tttctcgtaa ctaaaaacgc caaacattcc
     1261 cgattctatt ctataggtcg ggttatttga cgta
//
LOCUS       DARBET010000183         1287 bp    DNA     linear   BCT 07-NOV-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4027
            SAMEA114569841-rid20112183.denovo.190, whole genome shotgun
            sequence.
ACCESSION   DARBET010000183 DARBET010000000
VERSION     DARBET010000183.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA114569841
            Sequence Read Archive: ERR12203666
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1287)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1287)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001972618.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001972618.1
            Genome Coverage       :: 132x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/07/2023 13:18:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,041
            CDSs (total)                      :: 2,975
            Genes (coding)                    :: 2,908
            CDSs (with protein)               :: 2,908
            Genes (RNA)                       :: 66
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 67
            CDSs (without protein)            :: 67
            Pseudo Genes (ambiguous residues) :: 0 of 67
            Pseudo Genes (frameshifted)       :: 26 of 67
            Pseudo Genes (incomplete)         :: 28 of 67
            Pseudo Genes (internal stop)      :: 20 of 67
            Pseudo Genes (multiple problems)  :: 6 of 67
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1287
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA114569841-rid20112183.denovo.190"
                     /isolate="VRE4027"
                     /db_xref="taxon:1352"
                     /country="Denmark"
     gene            <1..848
                     /locus_tag="SCJ86_002907"
     CDS             <1..848
                     /locus_tag="SCJ86_002907"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000997693.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="IS256 family transposase"
                     /protein_id="HEG1170382.1"
                     /translation="EIADLIEKMYGHHYTPQTMSNITKSFTEEVTAFKGRELHDRYAA
                     IYMDATYIPLKRKTVAKEAIHIAVGIRPDGSKEVLSYAIAPTESITIWEEILLDLQER
                     GLKNVLLFITDGLKGMVGAISRFYPKARFQHCCVHVFRNISHKVRVNNRKEVCDDFKM
                     VYQASSKEVALEARGAFAKKWKTSYPKVVESILSNDHLLTFYDFPLAIRKSIYSTNLI
                     ESFNKQIKKYSHRKEQFQNEESMERFLVSSFDTYNQKFLGRSHKGFQQAEGELEQMLS
                     QPMEN"
BASE COUNT          418 a          235 c          252 g          382 t
ORIGIN      
        1 ctgaaattgc tgatttgatc gaaaaaatgt acggtcatca ctatactcca caaaccatgt
       61 ccaatataac aaaatcattt acagaagagg taacggcgtt taaagggcga gagcttcatg
      121 accgttatgc tgctatttat atggacgcaa cgtatattcc gttaaagcgg aaaaccgtcg
      181 ccaaggaagc tattcatatc gcagttggca ttcgcccgga cggatcaaag gaagtattga
      241 gctatgcgat tgcaccgact gaatccatca cgatttggga ggaaatttta ttagaccttc
      301 aagagcgcgg tttgaaaaat gtcctcctgt tcatcacgga tggcttaaag gggatggtag
      361 gagcgattag tcggttctat cccaaagctc gttttcaaca ttgttgtgta cacgtttttc
      421 gtaatatcag tcacaaagtg cgtgtcaata atcgtaagga agtctgtgat gattttaaaa
      481 tggtgtatca agcctcatct aaagaggtgg cgttagaagc acgtggtgct tttgcgaaaa
      541 aatggaaaac cagctatcca aaagtggttg aatcgattct ttcgaacgat cacttgctca
      601 ctttctatga tttccccttg gccatacgca agagtattta ttctacgaac ttgattgaat
      661 cctttaataa gcaaatcaag aaatacagcc accgcaagga acagttccaa aacgaagagt
      721 cgatggaacg tttcttagtc tcgtcttttg atacttacaa ccaaaaattc ctaggtcgca
      781 gtcataaagg tttccaacag gccgaaggcg agctcgaaca aatgctgagt caaccgatgg
      841 agaattagaa acaatctaca gtagggaaga accatttaca caaaattatt gacgctccca
      901 agctaaaact cctattaatc gatctactaa aaataaataa aaaaattctt ttcaattttt
      961 atttatggta tgacataaat aaagtaaagt tattcttcta ggttaatctt attaatactc
     1021 tttctcacta gtgttgcatg caacactagt gagaaagagt attaaatata ctgcaaggca
     1081 agacatttgg taattgaaga ataacaagca aaaaaatagc agtcaggtct cttgacaaag
     1141 tatagttgct attctttttt taggccctac tttaatgaaa tttttgtttg gagggaacga
     1201 cttcattcct ttgattattt cagttgaatt taatagagaa gcctggcgtc tattaagaaa
     1261 tacttttctt gatgagcgtc aattttt
//
LOCUS       DARBET010000184         1267 bp    DNA     linear   BCT 07-NOV-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4027
            SAMEA114569841-rid20112183.denovo.191, whole genome shotgun
            sequence.
ACCESSION   DARBET010000184 DARBET010000000
VERSION     DARBET010000184.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA114569841
            Sequence Read Archive: ERR12203666
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1267)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1267)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001972618.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001972618.1
            Genome Coverage       :: 132x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/07/2023 13:18:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,041
            CDSs (total)                      :: 2,975
            Genes (coding)                    :: 2,908
            CDSs (with protein)               :: 2,908
            Genes (RNA)                       :: 66
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 67
            CDSs (without protein)            :: 67
            Pseudo Genes (ambiguous residues) :: 0 of 67
            Pseudo Genes (frameshifted)       :: 26 of 67
            Pseudo Genes (incomplete)         :: 28 of 67
            Pseudo Genes (internal stop)      :: 20 of 67
            Pseudo Genes (multiple problems)  :: 6 of 67
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1267
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA114569841-rid20112183.denovo.191"
                     /isolate="VRE4027"
                     /db_xref="taxon:1352"
                     /country="Denmark"
     gene            22..1101
                     /locus_tag="SCJ86_002908"
     CDS             22..1101
                     /locus_tag="SCJ86_002908"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326691.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS30 family transposase"
                     /protein_id="HEG1170383.1"
                     /translation="MTQTHDNTVSRKGKHLSYSERCQIAILKKEGYSNRQVAKVLGRV
                     PQTINNEIDRGTITQLKRQTQNGKVYDTYYSVYDADAGQAFYEKQRLNCGRRPKWANT
                     NAFIDWADDKMLNEKWSPDVVVGFARKHELFDCAIIPGTTALYQWIDRGIMRTKNMDL
                     LEKLSRKIKAVRPGHRPNKRVLGTSIEERPKEVETRETFGHWEIDTVVGNKTKSDAVL
                     LTLAERQTRFEVILKLNGKDTESVDQAVQQLRERAGANFDQLFKTVTSDNGSEFSGLH
                     DALQDVADVYFSHPYASWERGTSENQHKLIRRFVPKGSPISEVSENQILRIQRWMNDY
                     PRKILGYATPHDTFVQAFKQERLVA"
BASE COUNT          378 a          301 c          310 g          278 t
ORIGIN      
        1 aatagacaag gatgaattca catgacgcaa acccatgata acacagtttc acgcaaggga
       61 aaacatcttt cctactcaga acgttgccaa atcgctatcc tgaaaaagga aggctattcc
      121 aaccgtcagg ttgccaaagt gctgggacgt gttccgcaaa ccataaacaa tgagattgat
      181 cgcggaacca tcacccagtt aaaacgccaa acgcaaaatg gaaaagtgta tgacacctac
      241 tattctgttt atgacgctga tgcaggacag gccttctatg aaaagcaacg actgaactgt
      301 ggcagacgcc ccaaatgggc gaacacaaat gccttcattg attgggcaga tgacaaaatg
      361 ttaaatgaaa aatggtcgcc ggacgtcgtg gtcggatttg ccagaaaaca cgagctgttc
      421 gattgtgcca tcattcctgg cacaaccgct ctttatcaat ggattgaccg cggaatcatg
      481 cgaacaaaga acatggactt gcttgaaaaa ctgtcccgta aaatcaaagc ggtccgccca
      541 ggacaccgtc ccaacaagcg tgttctcggg acgtcaattg aagaacgccc taaagaagtt
      601 gaaacacggg aaacgttcgg tcattgggaa atcgacacgg tagtgggaaa caagaccaag
      661 tcggatgccg tgctgctgac gctggctgaa cggcagacac gctttgaagt catcctaaag
      721 ttgaacggga aggacaccga atcggtggac caagcggttc aacagctaag agaacgggct
      781 ggcgctaact tcgatcaatt atttaagacc gttacatccg acaatggctc ggagttttct
      841 gggctacacg atgctctgca agatgtagct gacgtctatt tcagccaccc ctatgcttct
      901 tgggagcgtg ggacgagcga aaaccagcac aaattaattc gccgtttcgt ccccaaggga
      961 agcccgatta gtgaggtgag cgagaaccag attttgcgga tccagcgttg gatgaacgat
     1021 tacccgcgga aaattttggg ttatgcgaca ccacatgata cattcgtcca ggcattcaag
     1081 caagaacggt tggtggcttg acccattatt ccttgataac gatcctcact cgggacatgg
     1141 cttcatgtct ggaaagctgg cctggggcca gaatcgtctg aagactatca tacctcagtt
     1201 cattatcaag gcctcactga tttagaataa cgaggcagtt acggtatcaa acaaatgagt
     1261 tacctgt
//
LOCUS       DARBET010000185         1253 bp    DNA     linear   BCT 07-NOV-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4027
            SAMEA114569841-rid20112183.denovo.192, whole genome shotgun
            sequence.
ACCESSION   DARBET010000185 DARBET010000000
VERSION     DARBET010000185.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA114569841
            Sequence Read Archive: ERR12203666
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1253)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1253)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001972618.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001972618.1
            Genome Coverage       :: 132x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/07/2023 13:18:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,041
            CDSs (total)                      :: 2,975
            Genes (coding)                    :: 2,908
            CDSs (with protein)               :: 2,908
            Genes (RNA)                       :: 66
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 67
            CDSs (without protein)            :: 67
            Pseudo Genes (ambiguous residues) :: 0 of 67
            Pseudo Genes (frameshifted)       :: 26 of 67
            Pseudo Genes (incomplete)         :: 28 of 67
            Pseudo Genes (internal stop)      :: 20 of 67
            Pseudo Genes (multiple problems)  :: 6 of 67
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1253
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA114569841-rid20112183.denovo.192"
                     /isolate="VRE4027"
                     /db_xref="taxon:1352"
                     /country="Denmark"
     gene            <1..214
                     /locus_tag="SCJ86_002909"
     CDS             <1..214
                     /locus_tag="SCJ86_002909"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289370.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="acylneuraminate cytidylyltransferase family
                     protein"
                     /protein_id="HEG1170384.1"
                     /translation="MNASMYSFKPEFLKKSNYIFDGQCGIIKMRDYLILDIDSEEDFK
                     WLEFLFPKFLEDSSELQDIYNYLYRR"
     gene            219..1166
                     /locus_tag="SCJ86_002910"
     CDS             219..1166
                     /locus_tag="SCJ86_002910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014387016.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent epimerase/dehydratase family
                     protein"
                     /protein_id="HEG1170385.1"
                     /translation="MSCYLITGGAGFIGSTLANHLGEEHVVIVVDDLSMGKVENLDMT
                     KNITFIEGDVAEKSLMEEIMKANKFDYIFHLAAVASVADSVARPVETHRVNFESVLMI
                     LELIRKYQPDLKRIVFSSSAAVYGDEPTLPKKEESVIRPLTPYAVDKFAAEQYVLDYC
                     HLYDVPGSAVRFFNVYGPNQNPNSPYSGVISILVDRYKKQIVGEATSFTLFGDGSQSR
                     DFVYIDDVIQALLLVANEEAALGKQFNVGTGKATTLLELIHAINEILGVELTLEYKEE
                     RSGDIHDSLADITKIQSIGYQPKYGILSGMTNYLKTEIN"
BASE COUNT          411 a          179 c          261 g          402 t
ORIGIN      
        1 aatgaatgcc tcaatgtatt cttttaaacc tgaatttcta aaaaaaagta attatatttt
       61 tgatgggcaa tgtgggataa taaaaatgag agattatcta attttagata ttgatagtga
      121 agaagatttt aaatggttag aatttttgtt tcctaaattt ttagaagatt cttcggagct
      181 tcaagatatc tataattatt tatatcggag gtaaaaggat gagctgttat cttatcaccg
      241 gtggggctgg cttcattggt tctaccttag ccaatcattt aggagaagaa catgtggtaa
      301 tcgtcgtcga tgacttatca atgggaaaag tagaaaatct tgatatgacg aaaaatatca
      361 cttttattga gggagatgtc gctgagaaat ccttgatgga agagatcatg aaagctaata
      421 aatttgatta tatctttcat ttagcagcag ttgctagtgt ggcagattca gttgcccgac
      481 cagtagaaac acatcgtgtg aattttgaaa gtgtattaat gattttagaa ttgatccgca
      541 agtatcaacc tgatttaaaa cggatcgtgt ttagttcttc agcggctgtt tatggggatg
      601 aaccgacttt accaaaaaaa gaagagtccg tgattcgccc attgacgcct tatgcggttg
      661 ataaattcgc tgcagaacaa tatgtgctag attattgtca tttatatgat gtacccggta
      721 gcgcagttcg ttttttcaat gtctatggtc caaatcaaaa tccgaattct ccatacagtg
      781 gtgtgatttc tattctcgta gatcgctata agaaacaaat cgtaggagaa gcaacttcat
      841 ttacattatt tggtgatgga agccagtcta gagactttgt ttatatagat gatgtaatcc
      901 aagcattact tttagttgcg aatgaggaag ctgcactggg gaaacaattc aatgttggaa
      961 cgggtaaagc aaccacactt ttggaattga ttcatgcaat caatgaaatt ctcggtgtag
     1021 aattgacact tgaatacaaa gaagagcgtt cgggtgatat ccatgactct ctagcagata
     1081 ttactaagat ccagtcaatt ggctatcaac cgaaatatgg tattttaagt ggaatgacta
     1141 attatttgaa aactgaaatc aattaaaaca tactaagata agattagagg gtaaattcag
     1201 gctctttgtc aataaggact gatgagtttt gtaaaatcaa atctgggata gaa
//
LOCUS       DARBET010000186         1235 bp    DNA     linear   BCT 07-NOV-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4027
            SAMEA114569841-rid20112183.denovo.193, whole genome shotgun
            sequence.
ACCESSION   DARBET010000186 DARBET010000000
VERSION     DARBET010000186.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA114569841
            Sequence Read Archive: ERR12203666
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1235)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1235)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001972618.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001972618.1
            Genome Coverage       :: 132x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/07/2023 13:18:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,041
            CDSs (total)                      :: 2,975
            Genes (coding)                    :: 2,908
            CDSs (with protein)               :: 2,908
            Genes (RNA)                       :: 66
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 67
            CDSs (without protein)            :: 67
            Pseudo Genes (ambiguous residues) :: 0 of 67
            Pseudo Genes (frameshifted)       :: 26 of 67
            Pseudo Genes (incomplete)         :: 28 of 67
            Pseudo Genes (internal stop)      :: 20 of 67
            Pseudo Genes (multiple problems)  :: 6 of 67
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1235
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA114569841-rid20112183.denovo.193"
                     /isolate="VRE4027"
                     /db_xref="taxon:1352"
                     /country="Denmark"
     gene            <1..383
                     /locus_tag="SCJ86_002911"
     CDS             <1..383
                     /locus_tag="SCJ86_002911"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_086558187.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="IS3 family transposase"
                     /protein_id="HEG1170386.1"
                     /translation="FSRIKQPLTEVEIFHTDRGKEFDNQAIDELLTTFDINRSLSHKG
                     CPFDNAVAESTYKSLKVEFVYQYTFETLQQLDLELFDYVNWWNHLRLHGTLGYETPVG
                     YRNQRLAQRILDNELGCANASEAV"
     gene            complement(367..960)
                     /locus_tag="SCJ86_002912"
                     /pseudo
     CDS             complement(367..960)
                     /locus_tag="SCJ86_002912"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328085.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ParB-like nuclease domain-containing protein"
BASE COUNT          377 a          243 c          215 g          400 t
ORIGIN      
        1 catttagccg tatcaaacaa cctctgacag aggttgagat ttttcatact gatcgtggaa
       61 aagagtttga taaccaagct attgatgaat tattaacaac ttttgacatc aatcgatcat
      121 tgagtcataa aggctgtcct tttgataatg ccgtagctga atcaacttat aagtcgttga
      181 aagtagaatt tgtctatcaa tacacatttg aaaccttaca acaattggat ttggagttat
      241 ttgactatgt aaattggtgg aaccaccttc ggttgcacgg tacacttggc tacgagacac
      301 cggttggtta ccgtaaccag agattggcgc agcgaatcct tgataatgag ctcggatgtg
      361 ctaacgctag cgaggcagtc taactttaac tgttagctcc tgccgaagat cgtcacatcc
      421 gaggaggctc attgtcaagg acaatcggaa tagcatacgg aaagaagttc tgcaccttat
      481 aaaatttgtc aaaaaaactg ttgccattcc accagacact tgcttaaagc gaaaaatttc
      541 ttcaatgtcc atgcctaatt ctttggatat tctcttgtct gaccatccat tttttactaa
      601 tctttggatg acaatttcca ttcctcttat ttcatgggtc cctcttgcac gattgtgtcg
      661 aattgttgaa gccatacgtt cttatatggg tttatcaata actgttaccg gtacaacttt
      721 taagttgagt ctttccttag caacacgata cctatgaagc acatcaacca agatatactt
      781 ctcttctttt tccttgtaat aacaaactaa tggttgagta aagccatctt caatgataga
      841 aagctccaat aagttcattt ccggagacgc tactcgattg ggattataat cgttcgcctg
      901 tattttggta attggaacaa gccgaacgtc catcacaggg aaagttatgt ctttattcat
      961 acgcttgtat accctacttt cttaaaaagt agggtatact caagaatccg attaattatc
     1021 ttctcaattt ggataatata tctcatcagt cttcttgtca ctttatcaac atgattttgc
     1081 tgcaaaatgg gataccgaca gtactggact ggtaatcagg ataaaagcaa aaataattaa
     1141 tttgtatcct aaaaaatcca taaaaattct ctcacatttt cgggtattaa ttcgagaatc
     1201 acacctataa atttattact atataaggga tactt
//
LOCUS       DARBET010000187         1208 bp    DNA     linear   BCT 07-NOV-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4027
            SAMEA114569841-rid20112183.denovo.194, whole genome shotgun
            sequence.
ACCESSION   DARBET010000187 DARBET010000000
VERSION     DARBET010000187.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA114569841
            Sequence Read Archive: ERR12203666
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1208)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1208)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001972618.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001972618.1
            Genome Coverage       :: 132x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/07/2023 13:18:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,041
            CDSs (total)                      :: 2,975
            Genes (coding)                    :: 2,908
            CDSs (with protein)               :: 2,908
            Genes (RNA)                       :: 66
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 67
            CDSs (without protein)            :: 67
            Pseudo Genes (ambiguous residues) :: 0 of 67
            Pseudo Genes (frameshifted)       :: 26 of 67
            Pseudo Genes (incomplete)         :: 28 of 67
            Pseudo Genes (internal stop)      :: 20 of 67
            Pseudo Genes (multiple problems)  :: 6 of 67
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1208
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA114569841-rid20112183.denovo.194"
                     /isolate="VRE4027"
                     /db_xref="taxon:1352"
                     /country="Denmark"
     gene            <1..969
                     /locus_tag="SCJ86_002913"
     CDS             <1..969
                     /locus_tag="SCJ86_002913"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323939.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase"
                     /protein_id="HEG1170387.1"
                     /translation="PINTFAYTINDEYNLAPNQGDSRLAIPNKIILHETGIDAPARNV
                     AANMKNNYNGSNSYTTDVIGDGGIVYRVGEQGYVSWGAGNANPYAPVQIELQRTYDKA
                     LFEKNYRAYIEYTRDSAKKYGIPLTLDQGTSLFTKGIISHLWVTNYVWGNHTDPYGYL
                     SQMGVSKEKLAYDLAHGFTDENPTTSENKPVIDPTRAGAANPTLTDGTNHAHIDQFGE
                     IENANLHVAGWHIANYKYEYIFIMDYNTGKELARVRADGIYRPDVNQAYNTSGNVGYH
                     VSFNMRNFPSKKVYVMMRATNDPEGNTKGGAQDFHDKRWYLNIPKR"
BASE COUNT          403 a          219 c          245 g          341 t
ORIGIN      
        1 cccattaata cgtttgctta tactattaat gacgagtata atttagcgcc gaatcaagga
       61 gactccagat tagcaattcc taacaaaatt attttgcacg aaactggaat agatgcacca
      121 gcaagaaacg tagccgccaa catgaaaaat aattataacg gaagcaattc ttatactaca
      181 gatgttattg gtgacggtgg gattgtttac cgtgtgggtg agcaaggata tgtttcgtgg
      241 ggagctggta acgctaatcc ttatgcgcct gtacagattg aattacagcg cacatatgat
      301 aaagcattgt ttgaaaaaaa ctatcgagct tatattgaat atacaagaga tagtgcaaaa
      361 aaatatggaa ttccattgac tcttgatcaa ggaacttctt tatttacaaa aggaatcatt
      421 tctcatttgt gggtgacaaa ttatgtttgg gggaaccaca cagatccata tggttactta
      481 tcgcaaatgg gagttagcaa agaaaagctt gcttatgatt tagctcatgg atttaccgat
      541 gaaaatccaa caacttctga aaacaagcct gtcattgatc caacacgagc tggtgcagct
      601 aatcctacgc tgacagatgg aacaaatcac gcccacattg atcagtttgg ggaaatcgaa
      661 aacgcaaact tgcatgtggc tggatggcac attgctaact ataaatacga gtatattttc
      721 attatggact acaatactgg gaaagaatta gctcgagtaa gagctgatgg aatttataga
      781 ccagatgtaa accaagctta taatacttct ggaaatgttg gttatcatgt atctttcaat
      841 atgcgtaatt ttcctagtaa gaaagtctat gtaatgatgc gggcaacgaa tgatccagag
      901 ggaaacacta aaggcggtgc gcaagatttc catgacaaac gttggtattt aaatattcct
      961 aaacgataaa aatagctcct cgttgaggag cagtacataa ctatattgac aactataaaa
     1021 attattcgat aaaatagtga tgttatcgca tatcttcact atcacccata aatagtcaca
     1081 ctccaagcta tgcgataaca ggtttgttgc cacacattct actggttgat tgtttatggc
     1141 tttatgtggc aacaaccagt acccttagct cagttggtca gagcagacgg ctcataaccg
     1201 tccggtcg
//
LOCUS       DARBET010000188         1207 bp    DNA     linear   BCT 07-NOV-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4027
            SAMEA114569841-rid20112183.denovo.195, whole genome shotgun
            sequence.
ACCESSION   DARBET010000188 DARBET010000000
VERSION     DARBET010000188.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA114569841
            Sequence Read Archive: ERR12203666
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1207)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1207)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001972618.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001972618.1
            Genome Coverage       :: 132x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/07/2023 13:18:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,041
            CDSs (total)                      :: 2,975
            Genes (coding)                    :: 2,908
            CDSs (with protein)               :: 2,908
            Genes (RNA)                       :: 66
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 67
            CDSs (without protein)            :: 67
            Pseudo Genes (ambiguous residues) :: 0 of 67
            Pseudo Genes (frameshifted)       :: 26 of 67
            Pseudo Genes (incomplete)         :: 28 of 67
            Pseudo Genes (internal stop)      :: 20 of 67
            Pseudo Genes (multiple problems)  :: 6 of 67
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1207
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA114569841-rid20112183.denovo.195"
                     /isolate="VRE4027"
                     /db_xref="taxon:1352"
                     /country="Denmark"
     gene            <1..>1207
                     /locus_tag="SCJ86_002914"
     CDS             <1..>1207
                     /locus_tag="SCJ86_002914"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297185.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="ISL3-like element ISEfa5 family transposase"
                     /protein_id="HEG1170388.1"
                     /translation="QKKKTLIVDAVFSPAPHTCRNCGSTVVDGNGKVIVVKNGKKETI
                     VRFEQYNHMPLVMRLKKQRYTCKNCRTHWTAQSYFVQPRHSIANHVRYKIASLLTEKV
                     SLSFIAKNCQVSLTTVIRTLKEFKSYLPKQSKRILPRVLMVDEFRSHASIEDKMSFIC
                     ADGETGQLIDVLPTRKLPRLTSYFLGCANPEEVKFLVTDMNAAYFQLTKRILPNAKVV
                     IDRFHIIKHMNQAFNELRIREMNELRKVGQKSQAEKLKKNWRFLLKNRANINHYEYKT
                     WKSFRAPKYPFLTEAMMIDRLLGFSTSLKEAYPYFHELVEAFRDKDPDLFFSLLAELP
                     ETLDDGFREKLQNLLTYEEGITNAMIYPYTNGKIEAKNTHIKTMKRVSYGFKSFENMR
                     IRIFLINQL"
BASE COUNT          400 a          234 c          232 g          341 t
ORIGIN      
        1 ttcagaagaa aaagacgttg atcgtcgatg ctgttttctc acctgctccc catacctgta
       61 gaaattgtgg ttctactgtg gtagatggaa acgggaaagt aattgtagtg aaaaatggga
      121 agaaggaaac gattgtccgc tttgaacaat acaatcacat gcctttggtt atgcgcttaa
      181 aaaagcagcg ctatacctgt aaaaactgtc gaacccattg gacggctcaa agttattttg
      241 ttcaacccag acattcaatc gcgaatcatg tcagatataa aattgcttct ttactgactg
      301 aaaaagtatc tttatctttt attgcgaaaa attgtcaggt atctttgacc accgttattc
      361 gtacgttgaa agagtttaaa agctacttac caaagcaatc taagaggatt ctccccagag
      421 tattgatggt tgatgaattt cgttcgcacg cttccataga agataaaatg agctttattt
      481 gcgcagatgg cgaaacagga caattaattg atgtcttgcc tacgcgtaaa ttgcctcgat
      541 taacaagcta tttcttaggc tgcgctaatc ctgaagaagt aaaattttta gtcacagaca
      601 tgaacgccgc ctacttccag cttaccaaac gtattctgcc aaatgcgaaa gtagtgattg
      661 atcggtttca tattatcaaa cacatgaatc aagcctttaa tgagttgcgt atccgtgaaa
      721 tgaatgaact tcgtaaagta ggacagaaaa gtcaggcaga aaaactgaaa aagaactggc
      781 gctttttgct aaaaaatcgt gcaaacatca accattatga atacaaaaca tggaaaagtt
      841 ttcgagcacc aaaataccca tttcttacgg aagcgatgat gattgatcga ttgcttggct
      901 tttctacgtc cctgaaggag gcgtatccct attttcatga attagttgaa gcctttcgag
      961 acaaagaccc tgacttattt ttctccttat tggcagaact tcccgaaacg ttagatgacg
     1021 gctttcggga aaagctccaa aaccttctga cctatgaaga aggcatcacc aacgcgatga
     1081 tctatcctta caccaatgga aaaatagaag cgaagaatac ccatataaag acaatgaaac
     1141 gagtatccta cggatttaaa tcatttgaga acatgagaat tagaatcttt ttgatcaatc
     1201 aattaat
//
LOCUS       DARBET010000189         1202 bp    DNA     linear   BCT 07-NOV-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4027
            SAMEA114569841-rid20112183.denovo.196, whole genome shotgun
            sequence.
ACCESSION   DARBET010000189 DARBET010000000
VERSION     DARBET010000189.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA114569841
            Sequence Read Archive: ERR12203666
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1202)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1202)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001972618.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001972618.1
            Genome Coverage       :: 132x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/07/2023 13:18:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,041
            CDSs (total)                      :: 2,975
            Genes (coding)                    :: 2,908
            CDSs (with protein)               :: 2,908
            Genes (RNA)                       :: 66
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 67
            CDSs (without protein)            :: 67
            Pseudo Genes (ambiguous residues) :: 0 of 67
            Pseudo Genes (frameshifted)       :: 26 of 67
            Pseudo Genes (incomplete)         :: 28 of 67
            Pseudo Genes (internal stop)      :: 20 of 67
            Pseudo Genes (multiple problems)  :: 6 of 67
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1202
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA114569841-rid20112183.denovo.196"
                     /isolate="VRE4027"
                     /db_xref="taxon:1352"
                     /country="Denmark"
     gene            31..1044
                     /locus_tag="SCJ86_002915"
     CDS             31..1044
                     /locus_tag="SCJ86_002915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002416867.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation factor domain-containing
                     protein"
                     /protein_id="HEG1170389.1"
                     /translation="MESFNPELPLTLLFDYVRIRFPTTDARKIIQEILHLKFDYMLHE
                     DYAFYSYQEQYVMGDIVVMLSHEEDKGVLLELKGRGCRQFETFLLAQKRSWYDFFEDC
                     LKAGGVMKRLDLAINDRVGLLDIPDLTKKCQKEECISLFRTFKSYRSGELLKPDDKDG
                     MGNTLYIGSLKSEVYFCLYEKDYEQYIKLGIPLDQTETKNRFEIRLKNDRAYHAIQDL
                     LKGRSIESTTFSIINRYLRFADKVEGKRRTNWPLNEQWGRFIGRNQKEIQLTSEPKPY
                     TIERTLNWLGRQVAPTWKMAKELDRLNQTTYIQDMVNNTRLSDRHKKILEQQSMAIEN
                     LII"
     gene            1041..1178
                     /locus_tag="SCJ86_002916"
     CDS             1041..1178
                     /locus_tag="SCJ86_002916"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322329.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3789 domain-containing protein"
                     /protein_id="HEG1170390.1"
                     /translation="MKRGIVMEIVKDILLVFGGSMLGVTVMCLMHASAAADRAMEKKG
                     K"
BASE COUNT          383 a          195 c          284 g          340 t
ORIGIN      
        1 acaaaaacca tgaggaaaat ttttgaccaa ctggaaagtt tcaatcccga gttaccttta
       61 acgttactgt ttgactatgt aaggattcgc tttcccacaa cggatgcgag gaaaatcatt
      121 caagagattc tccatttgaa atttgattat atgctccacg aagattacgc cttttactcc
      181 tatcaagaac aatatgttat gggagatatt gtggtgatgt tgtcacatga agaagataaa
      241 ggcgtccttt tagaattaaa gggtcgtggt tgtcggcagt ttgaaacttt tttactcgcc
      301 caaaagcgta gctggtatga ctttttcgaa gattgtctaa aagccggtgg cgtgatgaaa
      361 cgcttagacc tggcaatcaa tgatcgagtt ggcctgttag atattcctga tttaacgaaa
      421 aaatgtcaga aggaagaatg tatctccttg tttcgtacct tcaaaagcta tcgttccggg
      481 gaactgttga aacctgatga taaagatggt atgggcaata ctttgtatat tggcagtctt
      541 aagagtgagg tctatttctg tttgtacgaa aaagactatg agcaatatat caagttaggg
      601 attcctttag accagaccga aacgaaaaat cgttttgaaa tccgactgaa aaatgatcga
      661 gcttatcatg caattcaaga tttgttaaaa ggtcgcagta ttgaaagtac cacattttcc
      721 attattaacc ggtacttgcg atttgcggat aaggtggaag gcaaaaggcg aactaactgg
      781 cccttgaatg aacaatgggg gcgcttcatt ggacggaatc agaaggaaat tcaattaact
      841 tctgaaccga agccttatac cattgaacga acgttgaact ggcttggacg acaagtcgcc
      901 cctacgtgga agatggccaa ggagttggat cggttgaatc agacaacgta cattcaggat
      961 atggtaaata atactcgact atcagaccgg cacaaaaaga ttttggagca acaaagtatg
     1021 gcaattgaaa atttgattat atgaaaagag gaatcgtgat ggaaattgtg aaagatattt
     1081 tattggtgtt tggtggcagt atgcttggtg ttactgtgat gtgcttgatg catgcaagtg
     1141 cagcggcgga ccgagcaatg gaaaagaaag gaaagtgatg aaatgaattt cggtcaaaat
     1201 tt
//
LOCUS       DARBET010000190         1195 bp    DNA     linear   BCT 07-NOV-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4027
            SAMEA114569841-rid20112183.denovo.197, whole genome shotgun
            sequence.
ACCESSION   DARBET010000190 DARBET010000000
VERSION     DARBET010000190.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA114569841
            Sequence Read Archive: ERR12203666
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1195)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1195)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001972618.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001972618.1
            Genome Coverage       :: 132x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/07/2023 13:18:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,041
            CDSs (total)                      :: 2,975
            Genes (coding)                    :: 2,908
            CDSs (with protein)               :: 2,908
            Genes (RNA)                       :: 66
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 67
            CDSs (without protein)            :: 67
            Pseudo Genes (ambiguous residues) :: 0 of 67
            Pseudo Genes (frameshifted)       :: 26 of 67
            Pseudo Genes (incomplete)         :: 28 of 67
            Pseudo Genes (internal stop)      :: 20 of 67
            Pseudo Genes (multiple problems)  :: 6 of 67
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1195
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA114569841-rid20112183.denovo.197"
                     /isolate="VRE4027"
                     /db_xref="taxon:1352"
                     /country="Denmark"
     gene            complement(25..162)
                     /locus_tag="SCJ86_002917"
     CDS             complement(25..162)
                     /locus_tag="SCJ86_002917"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322329.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3789 domain-containing protein"
                     /protein_id="HEG1170391.1"
                     /translation="MKRGIVMGIVKDIFLVFGGSMLGVTVMCLMNASAAADRAMEKKG
                     K"
     gene            complement(159..1172)
                     /locus_tag="SCJ86_002918"
     CDS             complement(159..1172)
                     /locus_tag="SCJ86_002918"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002416867.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation factor domain-containing
                     protein"
                     /protein_id="HEG1170392.1"
                     /translation="MESFNPDLPLTLLFDYVRIRFPTTDARKIIQEILHLKFEYMLHE
                     DYAFYSYQEQYVMGDIVVMLSHEEDKGVLLELKGRGCRQFETFLLAQKRSWYDFFEDC
                     LKAGGVMKRLDLAINDRVGLLDIPELTKKCQKEECISLFRTFKSYRSGELLKADEKDG
                     MGNTLYIGSLKSEVYFCLYEKDYEQYIKLGIPLDQTETKNRFEIRLKNDRAFHAIQDL
                     LKGRSIESTTFSIINRYLRFADKVEGKRRTNWPLNEQWGRFIGRNRKEIQLTSEPKPY
                     TIERTLNWLGRQVAPTWKMAKELDRLNRTTYIQDMVQNARLSDRHKKILEQQSMAIEN
                     LII"
BASE COUNT          341 a          286 c          191 g          377 t
ORIGIN      
        1 aaattttgtc cgaaattcat cttgtcactt tcctttcttt tccattgctc gatccgctgc
       61 tgcacttgca ttcatcaagc acatcacagt aacaccaagc atactgccac caaataccaa
      121 aaaaatatct tttacaattc ccatgacgat tcctcttttc atataatcaa attttcaatt
      181 gccatacttt gttgctccaa aatcttttta tgtcggtctg ataagcgtgc attctgtacc
      241 atatcctgga tataggttgt ccgattcaat cgatccaact ccttagccat tttccacgta
      301 ggggcgactt gtcggcctag ccaattcaag gttcgttcaa tggtatacgg ttttggctcg
      361 gaagttaatt gaatctcttt ccgattccgt ccaataaaac gcccccattg ttcattcaag
      421 ggccagttgg ttcgcctttt accttctact ttatccgcaa atcgcaagta acgattgata
      481 atggaaaacg tggtgctctc aatgctacga ccttttaaca aatcttgaat cgcatgaaaa
      541 gcacggtcat ttttcagtcg aatctcaaaa cgatttttcg tctcagtttg gtctaatgga
      601 atccctaact tgatatattg ttcatagtct ttttcataca aacagaaata aacctcactc
      661 ttgagactgc cgatatataa ggtattgccc atgccgtcct tttcatcagc ctttaaaagt
      721 tccccggaac gataactttt aaaggtacga aacaaggaga tacattcttc cttctgacat
      781 tttttcgtta gctcaggtat atccaatagc cccactcgat cattgattgc caagtccaag
      841 cgtttcatca cgccaccggc ctttagacaa tcctcgaaaa agtcgtacca gctacgcttt
      901 tgagcgagta aaaaagtttc aaattgtcgg caacctcgcc cctttaattc taaaagcaca
      961 cctttatctt cttcatgaga caacatcacc acaatatcac ccatgacata ctgttcttga
     1021 taggagtaaa aggcataatc ttcatggagc atatactcaa atttcaaatg gagaatttct
     1081 tgaataattt ttcgtgcatc cgttgtggga aagcgaatcc ttacatagtc aaacagtaac
     1141 gtcaaaggca aatcgggatt aaagctttcc agttggtcaa aaattttcct catgg
//
LOCUS       DARBET010000191         1195 bp    DNA     linear   BCT 07-NOV-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4027
            SAMEA114569841-rid20112183.denovo.198, whole genome shotgun
            sequence.
ACCESSION   DARBET010000191 DARBET010000000
VERSION     DARBET010000191.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA114569841
            Sequence Read Archive: ERR12203666
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1195)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1195)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001972618.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001972618.1
            Genome Coverage       :: 132x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/07/2023 13:18:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,041
            CDSs (total)                      :: 2,975
            Genes (coding)                    :: 2,908
            CDSs (with protein)               :: 2,908
            Genes (RNA)                       :: 66
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 67
            CDSs (without protein)            :: 67
            Pseudo Genes (ambiguous residues) :: 0 of 67
            Pseudo Genes (frameshifted)       :: 26 of 67
            Pseudo Genes (incomplete)         :: 28 of 67
            Pseudo Genes (internal stop)      :: 20 of 67
            Pseudo Genes (multiple problems)  :: 6 of 67
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1195
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA114569841-rid20112183.denovo.198"
                     /isolate="VRE4027"
                     /db_xref="taxon:1352"
                     /country="Denmark"
     gene            <1..1076
                     /locus_tag="SCJ86_002919"
     CDS             <1..1076
                     /locus_tag="SCJ86_002919"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014748673.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="IS110 family transposase"
                     /protein_id="HEG1170393.1"
                     /translation="VIGMESTSMYSFHPSMFFHEDEKLKKLNTLVTIENPFRVKQFSR
                     MFDENKTDRNDALRIADFLRIQRFTTSPIKEEKYMALQRLTRTRYQLIKQLIRTKQHF
                     LENLTYKCNTLAREMRDESTSLFSATLISLMTEDFTLDELAELPLEAFCDLLQEKGKG
                     RFKQPEKIAKAIQRAITMSYRLGALAQESINVVLSVLVREIRALEKNIKELDKAIEQV
                     IVVIPEYQCLTSIPGVGKVYAAGLIAEIGQIERFEDQTKLAKYAGLSWKVNQSGNYQS
                     QNTPLTKQGNRYFRYYLVEAANSVKNYLPEYKAFYQSKYKEVPKHQHKRALVLTARKF
                     TRLVDTLLRKHQLYTPPRSVIEK"
BASE COUNT          418 a          234 c          211 g          332 t
ORIGIN      
        1 ttgtgattgg catggagtca acgtccatgt acagcttcca tccttccatg ttttttcatg
       61 aagatgagaa actaaagaag ctcaacacac ttgtaacgat tgaaaatcct tttcgggtga
      121 aacaatttag tcgcatgttt gatgagaata aaactgatcg aaacgatgca ttaagaatcg
      181 ctgatttctt gcgtattcaa cgattcacaa cttcccctat caaagaagag aaatacatgg
      241 ccttacagcg tttaacacgt actcgctatc aacttattaa gcaattgatt cgtacaaagc
      301 aacattttct tgaaaatcta acgtacaaat gtaatacgct tgctcgagaa atgcgagacg
      361 aatcgacgag tctttttagt gcaaccctca tttcactcat gacggaagat ttcacattgg
      421 atgaacttgc tgagcttcct ttagaagctt tctgtgattt acttcaagaa aaaggaaaag
      481 gccgtttcaa gcaaccagaa aaaatcgcta aagcaattca acgtgcaatt accatgagtt
      541 accgcctagg tgctcttgca caagaatcaa ttaatgttgt tttaagtgtt ctcgtacgag
      601 aaatacgagc acttgaaaaa aatatcaaag aattagataa agccatcgaa caagtaattg
      661 ttgtcattcc agaatatcaa tgcttaacca gtattccagg cgttggtaaa gtctatgctg
      721 ccggtttaat cgctgaaatt ggtcaaatcg aaagatttga agatcagaca aaattggcaa
      781 aatacgctgg attaagttgg aaagtgaacc aatctggaaa ctatcagtcg caaaatacac
      841 ctctgacaaa acaaggaaat cgatatttta gatactactt agttgaagct gccaactctg
      901 taaaaaatta tcttcctgaa tacaaagctt tttatcaaag taaatataag gaggttccaa
      961 agcatcaaca caaacgtgca ctcgtcctaa ccgcaagaaa atttacgcgt ctggtggata
     1021 cgctactacg taaacaccaa ctctatacgc caccaaggag tgtgatagag aaataacaat
     1081 ccgttattga cgcaaatcct tgaatcaacc cgaaaaaaat ttgattttga tcgggtctag
     1141 tttcgtgcgc ttcaaaacat attactcaat aaagaaaatc ttaaatttca tcttg
//
LOCUS       DARBET010000192         1183 bp    DNA     linear   BCT 07-NOV-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4027
            SAMEA114569841-rid20112183.denovo.199, whole genome shotgun
            sequence.
ACCESSION   DARBET010000192 DARBET010000000
VERSION     DARBET010000192.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA114569841
            Sequence Read Archive: ERR12203666
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1183)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1183)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001972618.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001972618.1
            Genome Coverage       :: 132x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/07/2023 13:18:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,041
            CDSs (total)                      :: 2,975
            Genes (coding)                    :: 2,908
            CDSs (with protein)               :: 2,908
            Genes (RNA)                       :: 66
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 67
            CDSs (without protein)            :: 67
            Pseudo Genes (ambiguous residues) :: 0 of 67
            Pseudo Genes (frameshifted)       :: 26 of 67
            Pseudo Genes (incomplete)         :: 28 of 67
            Pseudo Genes (internal stop)      :: 20 of 67
            Pseudo Genes (multiple problems)  :: 6 of 67
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1183
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA114569841-rid20112183.denovo.199"
                     /isolate="VRE4027"
                     /db_xref="taxon:1352"
                     /country="Denmark"
     gene            <1..153
                     /locus_tag="SCJ86_002920"
     CDS             <1..153
                     /locus_tag="SCJ86_002920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611694.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS6 family transposase"
                     /protein_id="HEG1170394.1"
                     /translation="NKFYQSLRTASSTIKGMETIRGIYKKNRRNGTLFSFSVSTEIKV
                     LMGITA"
     gene            159..575
                     /locus_tag="SCJ86_002921"
     CDS             159..575
                     /locus_tag="SCJ86_002921"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEG1170395.1"
                     /translation="MEFREGAFDFQTSQQNRKFIPEDFQNLQLENMRLEDLFRLADDD
                     KYRFEVLKIKKNELLNKLYPENAIKAHFGDANHMYSWFIPKRFRGKINAGDQVKVLNR
                     NSTDVVMITDIYVEDEVIIKYMKPVIKLVNKNKGNG"
     gene            681..944
                     /locus_tag="SCJ86_002922"
     CDS             681..944
                     /locus_tag="SCJ86_002922"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system Phd/YefM family
                     antitoxin"
                     /protein_id="HEG1170396.1"
                     /translation="MAKTVLNPSSARKNFYKLLKDVNENHTEIEIISERSENNAVLIG
                     LDDWKAIKETLLLEQIGTLGVVRKREKDDSGFTNVDEIDWDNL"
     gene            944..>1183
                     /locus_tag="SCJ86_002923"
     CDS             944..>1183
                     /locus_tag="SCJ86_002923"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319468.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Txe/YoeB family addiction module toxin"
                     /protein_id="HEG1170397.1"
                     /translation="MNNYSVMIKNSAKADLKKIKQSNLKPQFEKVIQTLKEDPYLPTQ
                     SFEKLKPTHEGRYSRRLNRQHRVVYKVLLRSLKIKR"
BASE COUNT          448 a          154 c          239 g          342 t
ORIGIN      
        1 aataaatttt atcaaagtct ccgtacagcc tcttccacga ttaagggcat ggagaccatt
       61 cgaggaatat ataaaaagaa ccgaagaaat ggaacgctct tcagcttttc ggtgtctact
      121 gaaatcaagg tattaatggg aataacagcc taagatattt ggagttcaga gagggcgcgt
      181 ttgattttca aacttcgcaa cagaaccgaa aatttatacc ggaagatttt caaaacttac
      241 agttagaaaa catgcgttta gaagatttat ttagattagc agatgatgat aagtatagat
      301 ttgaagtttt gaaaattaaa aagaatgaac tattgaataa gttatatcct gagaatgcga
      361 taaaagcaca ttttggtgat gctaatcata tgtatagttg gttcattcct aaaagattta
      421 gagggaaaat caatgctgga gatcaagtga aagtactcaa tcgaaatagt acagatgtcg
      481 taatgataac agatatctac gtagaagatg aagttataat caaatatatg aagcctgtga
      541 taaaacttgt taataaaaat aaaggaaacg gataacactt tacaatgctg ttttggtact
      601 ccaatttaaa caaaacaatc gaatttacgt acttaattaa gtacgttaga atggagatat
      661 aaattagagg gggagtagat atggcaaaaa cagtcttaaa tcctagctca gcacggaaaa
      721 atttttataa gttgttgaaa gatgtaaatg agaatcatac ggaaattgaa atcattagtg
      781 agcgcagtga aaataatgcg gtgcttattg gtttggatga ttggaaagca atcaaagaaa
      841 cgctattact tgagcaaata ggcactttgg gtgttgttcg taagcgagaa aaagatgata
      901 gtggatttac taatgttgat gaaattgatt gggataactt ataatgaata actattctgt
      961 gatgattaaa aactcggcta aagctgattt aaagaaaatc aaacagtcta atttgaaacc
     1021 tcaatttgag aaggttattc agactttaaa ggaagatccc tatctcccta cccaatcatt
     1081 tgagaagtta aaacctacac atgaaggtag gtattctaga agattgaata gacaacaccg
     1141 ggttgtttat aaggttctgt tgcgaagttt gaaaatcaaa cgc
//
LOCUS       DARBET010000193         1161 bp    DNA     linear   BCT 07-NOV-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4027
            SAMEA114569841-rid20112183.denovo.200, whole genome shotgun
            sequence.
ACCESSION   DARBET010000193 DARBET010000000
VERSION     DARBET010000193.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA114569841
            Sequence Read Archive: ERR12203666
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1161)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1161)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001972618.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001972618.1
            Genome Coverage       :: 132x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/07/2023 13:18:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,041
            CDSs (total)                      :: 2,975
            Genes (coding)                    :: 2,908
            CDSs (with protein)               :: 2,908
            Genes (RNA)                       :: 66
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 67
            CDSs (without protein)            :: 67
            Pseudo Genes (ambiguous residues) :: 0 of 67
            Pseudo Genes (frameshifted)       :: 26 of 67
            Pseudo Genes (incomplete)         :: 28 of 67
            Pseudo Genes (internal stop)      :: 20 of 67
            Pseudo Genes (multiple problems)  :: 6 of 67
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1161
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA114569841-rid20112183.denovo.200"
                     /isolate="VRE4027"
                     /db_xref="taxon:1352"
                     /country="Denmark"
     gene            <1..>1161
                     /locus_tag="SCJ86_002924"
     CDS             <1..>1161
                     /locus_tag="SCJ86_002924"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="ISL3-like element ISEfa11 family transposase"
                     /protein_id="HEG1170398.1"
                     /translation="QKKKTLVVDAVLSPTPRACRSCGSTVVDGNGKAIIVKNGKKETI
                     VRFEQYNHMPLVMRLKKQRYTCKNCRTHWTAQSYFVQPRHSIANHVRYKIASLLTEKV
                     SLSFIAKSCQVSLTTVIRTLKEFKSYLPKQSKKILPRVLMVDEFRSHASIEDKMSFIC
                     ADGETGKLIDVLPTRKLPRLTSYFLGCTNPEEVEFLVTDMNAAYFQLTKRVLPNAKVV
                     IDRFHIVKHMNQAFNELRIREMNELRKAGQKSQAEKLKKNWRFLLKNRANINHYEYKT
                     WKSFRAPKYPFLTEAMMIDRLLEFSPPLKEAYPFFHELVEAFRDKDPDLFFSLLAELP
                     ETLDDSFREKLQNLLTYEEGITNAMIYPYSNGKIEAKNTHIKTMKRVSYGFK"
BASE COUNT          382 a          233 c          225 g          321 t
ORIGIN      
        1 ttcagaagaa aaagacgttg gtcgtcgatg ctgttctctc gcctactcct cgtgcttgta
       61 gaagttgtgg ttctactgtg gtagatggaa acgggaaagc aattatagtg aaaaatggaa
      121 aaaaggaaac gattgtccgt tttgaacaat acaatcatat gcctttggtt atgcgcctaa
      181 aaaagcaacg ctatacctgt aaaaactgtc gcacccattg gacggctcaa agttattttg
      241 tccaacccag acattcaatc gcaaatcatg ttagatataa aattgcttct ttactgactg
      301 aaaaagtatc tttatctttt attgcgaaaa gctgtcaggt atctttgacc accgttattc
      361 gtacattgaa agagtttaaa agctatttac caaagcaatc taagaagatc ctccccagag
      421 tattgatggt tgacgaattt cgttcgcatg cttccataga agataagatg agctttattt
      481 gcgcggatgg cgaaacagga aaattaatag atgttttgcc tacgcgtaaa ttacctcgat
      541 taacaagcta tttcttaggt tgtaccaatc cagaagaagt agaattcttg gtgacagaca
      601 tgaacgccgc ctacttccag ctcaccaaac gtgttctgcc aaatgcgaaa gtcgtgattg
      661 atcggtttca tattgtcaaa cacatgaatc aagcgttcaa tgagttgcgt atccgtgaaa
      721 tgaatgaact tcgtaaagcc ggacagaaaa gccaggcaga aaaactgaaa aagaactggc
      781 gctttttgct aaaaaatcgt gcaaacatca accattatga atacaaaaca tggaaaagtt
      841 tccgagcacc aaaataccca tttcttactg aagcaatgat gattgatcga ttgcttgagt
      901 tttctccgcc cctaaaggag gcgtatccct tttttcatga attagttgaa gcctttcgag
      961 acaaagaccc tgacttattt ttctccttat tggcagaact tcccgaaacg ttagatgaca
     1021 gctttcggga aaagcttcaa aaccttctga cctatgaaga aggcatcacc aacgcaatga
     1081 tctatcctta ttccaatgga aaaatagaag cgaagaatac ccacataaag acaatgaaac
     1141 gagtatccta cggatttaaa t
//
LOCUS       DARBET010000194         1160 bp    DNA     linear   BCT 07-NOV-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4027
            SAMEA114569841-rid20112183.denovo.201, whole genome shotgun
            sequence.
ACCESSION   DARBET010000194 DARBET010000000
VERSION     DARBET010000194.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA114569841
            Sequence Read Archive: ERR12203666
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1160)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1160)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001972618.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001972618.1
            Genome Coverage       :: 132x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/07/2023 13:18:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,041
            CDSs (total)                      :: 2,975
            Genes (coding)                    :: 2,908
            CDSs (with protein)               :: 2,908
            Genes (RNA)                       :: 66
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 67
            CDSs (without protein)            :: 67
            Pseudo Genes (ambiguous residues) :: 0 of 67
            Pseudo Genes (frameshifted)       :: 26 of 67
            Pseudo Genes (incomplete)         :: 28 of 67
            Pseudo Genes (internal stop)      :: 20 of 67
            Pseudo Genes (multiple problems)  :: 6 of 67
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1160
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA114569841-rid20112183.denovo.201"
                     /isolate="VRE4027"
                     /db_xref="taxon:1352"
                     /country="Denmark"
     gene            <1..704
                     /locus_tag="SCJ86_002925"
     CDS             <1..704
                     /locus_tag="SCJ86_002925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287871.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="IS256 family transposase"
                     /protein_id="HEG1170399.1"
                     /translation="YSVIFMDATHIPVKRQTVSKEAVYITIGIRLDGTKEVLGFTIAP
                     TESAYIWKEVLQDLRKRGLEEVLLVVTDGLSCIEESIHSVYPNAQFQQCCVHVSRNIA
                     HKVRVRGRKEICEDFKLVYQANSKEEALDHIDFMTRKWKKQYPRVVNLLLNPALLTFY
                     NFPHAIRRTIYSTNLIEGFNKQLKRYTRRKEQFPNEESLERFFISQFNQYNQKFLGRI
                     HKGFKEIQDTLESMI"
     gene            complement(1044..>1160)
                     /locus_tag="SCJ86_002926"
     CDS             complement(1044..>1160)
                     /locus_tag="SCJ86_002926"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010868911.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS6 family transposase"
                     /protein_id="HEG1170400.1"
                     /translation="TIKGMEAIRGLYKKTRKEGTLFGFSVCTEIKVLLGIPA"
BASE COUNT          383 a          195 c          195 g          387 t
ORIGIN      
        1 attattccgt tatatttatg gatgcgacgc atatccctgt gaagcgacag actgtttcga
       61 aggaagcagt gtatattacg attggtattc gtttggatgg aaccaaagaa gttcttgggt
      121 ttactattgc cccaactgag tctgcttata tttggaaaga agttcttcaa gatcttagaa
      181 aacgtgggtt agaagaagtt ttattagtag tgacagacgg attaagctgt attgaagaaa
      241 gtatccatag tgtgtatccg aatgcccaat ttcaacaatg ttgtgtgcat gtatctagaa
      301 atatcgctca taaagttcgt gttcgaggtc gaaaagaaat ttgtgaggat ttcaaattgg
      361 tttaccaagc gaattcaaaa gaagaggcat tggatcacat cgactttatg actaggaaat
      421 ggaaaaagca gtatccaaga gtcgtcaatt tactcttgaa tcctgcccta ttaacctttt
      481 ataatttccc tcacgccatc agacgaacaa tttattcgac gaacctgatt gaaggcttta
      541 ataagcagct aaaacgatat actcgaagaa aagaacaatt ccctaatgaa gaatctctag
      601 agagattctt catttctcaa tttaatcaat ataaccaaaa atttttaggt agaattcaca
      661 agggatttaa agaaattcag gatacattag agtcgatgat ttaactgtaa ttaacagaat
      721 ggattttcca tttacatata attcttgacg ctaccataat ctaaataacc atcatattca
      781 tctactactt atataattga gttttattaa actttttaag tttctattct atcaaatatt
      841 taaacaatct ctgcatccga aaagactacc tcgtatagtt ttactaacaa tatttttatt
      901 agcgtgttca aatcctgatt tcttgtatca attattttct gttcactttc aataagaaaa
      961 aagtctttac ttccatggat aataataagg gttctgttgc aaagttttaa ataaagaata
     1021 aaatccctta cggtatctat gatttaagct gggattccca ataatacctt gatttcagta
     1081 cagaccgaaa acccgaagag agtgccttct tttcgggttt tcttatataa tcctcggatg
     1141 gcttccatgc ctttaatcgt
//
LOCUS       DARBET010000195         1159 bp    DNA     linear   BCT 07-NOV-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4027
            SAMEA114569841-rid20112183.denovo.202, whole genome shotgun
            sequence.
ACCESSION   DARBET010000195 DARBET010000000
VERSION     DARBET010000195.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA114569841
            Sequence Read Archive: ERR12203666
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1159)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1159)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001972618.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001972618.1
            Genome Coverage       :: 132x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/07/2023 13:18:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,041
            CDSs (total)                      :: 2,975
            Genes (coding)                    :: 2,908
            CDSs (with protein)               :: 2,908
            Genes (RNA)                       :: 66
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 67
            CDSs (without protein)            :: 67
            Pseudo Genes (ambiguous residues) :: 0 of 67
            Pseudo Genes (frameshifted)       :: 26 of 67
            Pseudo Genes (incomplete)         :: 28 of 67
            Pseudo Genes (internal stop)      :: 20 of 67
            Pseudo Genes (multiple problems)  :: 6 of 67
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1159
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA114569841-rid20112183.denovo.202"
                     /isolate="VRE4027"
                     /db_xref="taxon:1352"
                     /country="Denmark"
     gene            <1..791
                     /locus_tag="SCJ86_002927"
     CDS             <1..791
                     /locus_tag="SCJ86_002927"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292877.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="O-antigen ligase family protein"
                     /protein_id="HEG1170401.1"
                     /translation="FIVQIFYITLSSSRGAQVVICSFLVLFLLLFANSKFRKSIREVL
                     PVKIKWMVSILLVLLVYFSFGTNYAKDFLATIPVSIDRKVEVIESNESDKTPLPEKEV
                     ILQHSSDNSEISAGRFTIWKAALKVIRPTLLFGYGETDFYGNSDAKFLKKVDLDTTDV
                     NELKRAHGNMHNGFLQVLVSSGIIAFICIYAFYVLNIFSLLKTFFRSKVNYAFLGIVL
                     IFILSIFIDEMVEAHVLFNKRDVISIVFWYYLGSISYLYLKKEN"
     gene            793..>1159
                     /locus_tag="SCJ86_002928"
     CDS             793..>1159
                     /locus_tag="SCJ86_002928"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEG1170402.1"
                     /translation="MKTAFIAWTPLHLINILNVYQNFYRESKGDLFIYSEFENARRYS
                     ERIKELGLFNDVVFVNHRKIGSSFKRKINLLTNHNQFFSDDLSVYDEIFIQGENYFTK
                     ILFSNVYKKNPDVKLNYIED"
BASE COUNT          388 a          140 c          192 g          439 t
ORIGIN      
        1 attttatcgt acaaatattt tatattacac tttcttcgtc tagaggtgcc caggtagtaa
       61 tttgttcttt tttagtatta tttcttttgt tgtttgccaa ttcaaaattt agaaagagca
      121 ttagagaagt actacctgta aaaatcaaat ggatggtaag catattatta gttctattgg
      181 tatatttttc ttttggtact aattatgcta aggatttttt agcaacaatt cctgtatcga
      241 ttgatagaaa agtagaagtt atagagtcaa atgaatcaga taaaactcca cttccagaaa
      301 aagaagtaat attacagcat tctagtgata actccgaaat ttcagctggt cgttttacga
      361 tttggaaagc tgcgcttaaa gttataagac caactttgtt atttggttat ggagagaccg
      421 atttctatgg gaatagcgat gcaaaatttt tgaaaaaagt agatcttgat actacagatg
      481 tgaatgaact aaaaagagca catggaaaca tgcataatgg atttcttcaa gtacttgtat
      541 caagtggtat aattgctttt atatgtatct atgctttcta tgtgttaaat atctttagtt
      601 tactcaaaac attttttaga agtaaagtga attatgcgtt tcttggtatt gttttaattt
      661 ttatactatc tatttttata gatgaaatgg tggaggctca tgtacttttc aataagcgcg
      721 atgtcatttc aattgttttc tggtattatt tgggaagcat ttcttacttg tatttaaaaa
      781 aggagaatta atatgaagac agctttcatt gcttggactc ctttacattt gattaatata
      841 ttaaatgttt atcaaaattt ttaccgagaa tcaaaaggag atttatttat ttattctgaa
      901 tttgaaaacg ctagaagata tagtgagcgt ataaaagaat tagggctatt taatgatgtt
      961 gtttttgtga atcaccggaa aatcggttct tcctttaaac gtaaaattaa cttgttaact
     1021 aaccataatc aatttttctc ggatgattta tccgtttatg atgaaatttt tatacagggg
     1081 gaaaattatt tcacaaaaat acttttctcc aatgtttaca aaaaaaatcc agatgtaaaa
     1141 ctaaattata tagaagatg
//
LOCUS       DARBET010000196         1134 bp    DNA     linear   BCT 07-NOV-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4027
            SAMEA114569841-rid20112183.denovo.203, whole genome shotgun
            sequence.
ACCESSION   DARBET010000196 DARBET010000000
VERSION     DARBET010000196.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA114569841
            Sequence Read Archive: ERR12203666
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1134)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1134)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001972618.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001972618.1
            Genome Coverage       :: 132x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/07/2023 13:18:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,041
            CDSs (total)                      :: 2,975
            Genes (coding)                    :: 2,908
            CDSs (with protein)               :: 2,908
            Genes (RNA)                       :: 66
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 67
            CDSs (without protein)            :: 67
            Pseudo Genes (ambiguous residues) :: 0 of 67
            Pseudo Genes (frameshifted)       :: 26 of 67
            Pseudo Genes (incomplete)         :: 28 of 67
            Pseudo Genes (internal stop)      :: 20 of 67
            Pseudo Genes (multiple problems)  :: 6 of 67
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1134
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA114569841-rid20112183.denovo.203"
                     /isolate="VRE4027"
                     /db_xref="taxon:1352"
                     /country="Denmark"
     gene            complement(19..924)
                     /gene="xerC"
                     /locus_tag="SCJ86_002929"
     CDS             complement(19..924)
                     /gene="xerC"
                     /locus_tag="SCJ86_002929"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008264721.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine recombinase XerC"
                     /protein_id="HEG1170403.1"
                     /translation="MTEATWEQKFLRYLIVERGYSEKTKEAYEEDILHFKHFLEESGD
                     ADLLKAEYFTVRVYLSALADHRPSYSRNSISRKIASLRSFYQYLLKEEVIKENPFSYV
                     HLKKKNLRLPRFFYENEMQALFDSVAGDSPLDLRNRALLEVLYGSGIRLSECSSLSVS
                     DIDFDSEVMLIHGKGNKERYAPLGSFAQDALQEYFREGRQVLMTKYHEEHDIVFVNHH
                     GKPITPTGIEYVLNQMIKKSSLDSDIHPHMLRHTFATHLLNNGADLRTVQELLGHANL
                     STTQIYAHVTKESLQKNYRSFHPRA"
BASE COUNT          336 a          231 c          194 g          373 t
ORIGIN      
        1 ttgttctttt ccttattttt acgctcttgg atggaaagaa cgatagtttt tttgtaaact
       61 ttcttttgtt acatgggcat agatctgggt agtcgataaa ttggcatgtc ctagcaattc
      121 ttggactgtc cgcaaatctg ccccattatt taataaatga gtagcaaatg tatggcgcag
      181 catgtgagga tggatatcac tatccaaact gctttttttg atcatttgat tcaagacata
      241 ttcaattcca gtaggcgtga ttggttttcc atgatgattc acaaaaacga tatcatgctc
      301 ttcgtgatat tttgtcatca atacctggcg gccttccctg aaatattctt gcagagcatc
      361 ttgagcaaaa gaacctagag gagcatatct ctctttgttc cctttaccat ggatcaacat
      421 gacctctgaa tcaaaatcaa tgtctgatac cgacaaactg ctacactcac ttaaacggat
      481 gcctgacccg taaaggactt ccagtaaagc gcggttcctc aaatccaaag gactatctcc
      541 tgctacgctg tcaaaaagtg cttgcatttc attttcataa aaaaaacgtg gcagtctcaa
      601 atttttcttt ttgagatgaa cgtaagaaaa tggattttct tttatcactt cttctttgag
      661 aagatactga taaaacgaac gtaggctggc tatcttgcga ctgatagaat ttctgctata
      721 gctaggacga tgatcagcta atgcactcaa atagacacga accgtaaaat attccgcctt
      781 cagcagatca gcgtctccag attcctccaa aaaatgtttg aaatggagaa tgtcttcttc
      841 atatgcttct ttcgtttttt ctgaatatcc tctttcgaca atcaaataac gcaaaaattt
      901 ttgttcccac gtggcttctg tcattttctc accttccttt tgtcccagag aataatttag
      961 cacagcgttt ataaaaagac aaataaattg tcagaattta accaatattt ctaagtttgt
     1021 tcacaattgg cgcaattcct cacattttgt ttgagaaatt cttaagaaaa acgcaagaaa
     1081 aagggctcta taatttatgg gactgatgaa tcagaattga ttcttcagcc ccat
//
LOCUS       DARBET010000197         1122 bp    DNA     linear   BCT 07-NOV-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4027
            SAMEA114569841-rid20112183.denovo.204, whole genome shotgun
            sequence.
ACCESSION   DARBET010000197 DARBET010000000
VERSION     DARBET010000197.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA114569841
            Sequence Read Archive: ERR12203666
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1122)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1122)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001972618.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001972618.1
            Genome Coverage       :: 132x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/07/2023 13:18:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,041
            CDSs (total)                      :: 2,975
            Genes (coding)                    :: 2,908
            CDSs (with protein)               :: 2,908
            Genes (RNA)                       :: 66
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 67
            CDSs (without protein)            :: 67
            Pseudo Genes (ambiguous residues) :: 0 of 67
            Pseudo Genes (frameshifted)       :: 26 of 67
            Pseudo Genes (incomplete)         :: 28 of 67
            Pseudo Genes (internal stop)      :: 20 of 67
            Pseudo Genes (multiple problems)  :: 6 of 67
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1122
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA114569841-rid20112183.denovo.204"
                     /isolate="VRE4027"
                     /db_xref="taxon:1352"
                     /country="Denmark"
     gene            116..808
                     /locus_tag="SCJ86_002930"
     CDS             116..808
                     /locus_tag="SCJ86_002930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit omega"
                     /protein_id="HEG1170404.1"
                     /translation="MRKKIIINAGVIIGLMLVIFSGKQYADNILLWGGEDNIEKINNN
                     LTILDTALENKEQKISILNSQLSSNNQTLEQLRTNIEVSKQKVASLENDKNQLVSEKT
                     NLENQLSAKDSELQGKQNEINAKIDEINQKVAEINRLNSDWNNRLQNEKNKFNEEQST
                     INSLNQQINQMLSEKNNIQNQLQATQQKLEEEKSKNSSLNNYIEKLQKAKNDVEDTAN
                     KAQQIVNEHTIQ"
BASE COUNT          495 a          126 c          165 g          336 t
ORIGIN      
        1 tatccttttt aaataatttt ttagacaaaa aacagaaagt gtctaaagaa agcttgacga
       61 agaaaataat ttttaataac ttatttaagg aaattttttg aaggagaaga taaaagtgag
      121 aaaaaagatt attattaatg ctggagtaat tataggattg atgttagtaa ttttctccgg
      181 aaagcaatat gctgataata ttttattatg gggaggggaa gataatattg aaaagatcaa
      241 caacaattta actatattag acacagctct agaaaataaa gagcaaaaaa ttagtatttt
      301 aaattcacaa ttgtcttcaa acaaccaaac tcttgaacaa ctaagaacta atattgaagt
      361 ttccaaacaa aaagtggcat ctttagaaaa cgacaagaat caattagtat cagagaaaac
      421 taatttagaa aatcaattgt cagcaaaaga ctctgaattg caaggaaaac aaaatgaaat
      481 taatgcgaaa atagacgaaa ttaaccaaaa agtagctgaa ataaatcgat taaatagcga
      541 ttggaataat cgtttacaaa atgaaaagaa caaatttaat gaagaacaat ctactattaa
      601 tagtttaaac cagcaaatta atcaaatgtt gagtgaaaaa aataacattc aaaatcaact
      661 gcaagctact cagcaaaagc tagaagaaga aaagtcaaaa aatagtagtt tgaataatta
      721 cattgaaaag cttcaaaaag ctaaaaatga tgtagaggat accgcaaata aagcacaaca
      781 aattgtaaat gaacatacaa tccaatagat atagttttaa tttaacaaat ggtatgtttg
      841 gagataatga gatagaatat aaatgtcata atattataca atctaaacac cgtttccttt
      901 tctatttttg atgacatcag tatatagcta ctcgaaagag tagtcttttt attattctcg
      961 cttattattg atatgaaaat ttcgatttga caaaaggtac cgaataattt tgtaatatga
     1021 attattatca agaagtaatt atgatgataa catatggaaa ctagttagta ataataacag
     1081 gtgattaaac ttttgaacga cttgtaattc tatatttttt ct
//
LOCUS       DARBET010000198         1115 bp    DNA     linear   BCT 07-NOV-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4027
            SAMEA114569841-rid20112183.denovo.205, whole genome shotgun
            sequence.
ACCESSION   DARBET010000198 DARBET010000000
VERSION     DARBET010000198.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA114569841
            Sequence Read Archive: ERR12203666
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1115)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1115)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001972618.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001972618.1
            Genome Coverage       :: 132x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/07/2023 13:18:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,041
            CDSs (total)                      :: 2,975
            Genes (coding)                    :: 2,908
            CDSs (with protein)               :: 2,908
            Genes (RNA)                       :: 66
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 67
            CDSs (without protein)            :: 67
            Pseudo Genes (ambiguous residues) :: 0 of 67
            Pseudo Genes (frameshifted)       :: 26 of 67
            Pseudo Genes (incomplete)         :: 28 of 67
            Pseudo Genes (internal stop)      :: 20 of 67
            Pseudo Genes (multiple problems)  :: 6 of 67
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1115
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA114569841-rid20112183.denovo.205"
                     /isolate="VRE4027"
                     /db_xref="taxon:1352"
                     /country="Denmark"
     gene            14..>1115
                     /locus_tag="SCJ86_002931"
     CDS             14..>1115
                     /locus_tag="SCJ86_002931"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349576.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEG1170405.1"
                     /translation="MRWYMNLKKIFSVKIFIVYAFVSLLYLFPAFKNMEIFGFYDTAF
                     HINRALSLESIFSSPINFETFRSYGMQVNNFYPWLTLYPLFLLIKFTNLAIGYNLFLY
                     IVTLITLFICHYVMYEITKKHVTSSFFAIIYTTSSFRSVEIFLRGAMGELLAMSILPL
                     ILLGFIKLYDSKKESWVMLAISMTLLIYTHVLSVAMTMVMIIIALIIQFYRKKKIEIF
                     LIIKLIKAAIVTLLLSLAFIGPMIEQTLYQDLNRPYVDILQKRAIYLGKEFLIGSIDS
                     ELLSFGIGLTLLICLIILTFNFKKLGILSKITYIMGIISILLCTKIFPWFALQNTPLN
                     IIQAPWRFMIFSTLFISFSVSLFISPQLEKFSY"
BASE COUNT          358 a          151 c          149 g          457 t
ORIGIN      
        1 aatataaaaa aacatgaggt ggtatatgaa tttgaaaaaa attttttctg taaaaatatt
       61 tatagtttat gcatttgtta gtttattata tttatttcca gcatttaaaa acatggaaat
      121 atttggcttt tatgatacgg catttcacat taatcgtgcg ttaagtttag aatctatttt
      181 tagctcccct attaattttg aaacgtttag atcatatggc atgcaagtaa ataattttta
      241 cccatggtta actctatatc ctttattttt attgatcaag tttactaatt tagcaatagg
      301 ctataacctt tttctttata ttgttacgct cattactcta tttatatgtc attatgtaat
      361 gtatgaaatt accaaaaaac atgttactag ctcctttttc gctattatat atacaacatc
      421 ttcttttagg agtgtagaaa tatttcttcg aggggcaatg ggggaattac tcgcaatgtc
      481 aatacttcct cttattttac tcgggtttat aaagctttac gactcaaaaa aagaatcttg
      541 ggttatgttg gcgattagca tgactttatt aatttacact catgttcttt ctgtagctat
      601 gactatggta atgattataa ttgctttaat tattcaattt tatcggaaaa agaaaataga
      661 aatcttctta attattaaat taataaaagc tgctatagtt actttacttc tttcactagc
      721 gtttataggt cctatgattg agcagacatt gtatcaagat ctgaatagac cttatgtgga
      781 tatattacaa aaaagagcaa tttatctggg taaagagttt ttgattggaa gtatagattc
      841 agaattacta tcatttggta ttggcttaac tttgcttatt tgtttaatta tactaacatt
      901 taactttaaa aaattaggaa tcttatctaa aatcacttat ataatgggta taatatctat
      961 tctactttgt acaaaaatat ttccatggtt tgccttacaa aatactcctt taaatattat
     1021 tcaagctcct tggcgtttta tgatattttc aacattgttc atatcttttt cagtatctct
     1081 atttatttct ccacagttgg aaaaattttc ttata
//
LOCUS       DARBET010000199         1105 bp    DNA     linear   BCT 07-NOV-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4027
            SAMEA114569841-rid20112183.denovo.206, whole genome shotgun
            sequence.
ACCESSION   DARBET010000199 DARBET010000000
VERSION     DARBET010000199.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA114569841
            Sequence Read Archive: ERR12203666
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1105)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1105)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001972618.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001972618.1
            Genome Coverage       :: 132x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/07/2023 13:18:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,041
            CDSs (total)                      :: 2,975
            Genes (coding)                    :: 2,908
            CDSs (with protein)               :: 2,908
            Genes (RNA)                       :: 66
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 67
            CDSs (without protein)            :: 67
            Pseudo Genes (ambiguous residues) :: 0 of 67
            Pseudo Genes (frameshifted)       :: 26 of 67
            Pseudo Genes (incomplete)         :: 28 of 67
            Pseudo Genes (internal stop)      :: 20 of 67
            Pseudo Genes (multiple problems)  :: 6 of 67
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1105
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA114569841-rid20112183.denovo.206"
                     /isolate="VRE4027"
                     /db_xref="taxon:1352"
                     /country="Denmark"
     gene            <1..1087
                     /locus_tag="SCJ86_002932"
     CDS             <1..1087
                     /locus_tag="SCJ86_002932"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309068.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HEG1170406.1"
                     /translation="DINDDGSRKKVEKVGGDTRPEAEAALRKVLSDIDETGQYFLGTD
                     TRVKQYLDFWMEEYVKLNLKYNTYENYRFTIKNHINGYLGKKKLTDLSPALLQNFINA
                     EFKKGYSKKTMTITHSVLKNALNMAVYPWGLIKQNPMLYVKIPKYEARPTTKKDLKII
                     SLEDFDHMLEITPEGHPFYIPLNIGFYTGMRVGEVCGLTWDDVDFSNGTITVEKQMVK
                     NDGAWVYGTPKTSSSNRTIFIGQTLLAILKKHKKQQLENRMKYGKLYIDSNAVCTKED
                     GELVTPSVVKWNTRRISNALSLSFNFHSLRHTHATLLLENGAKMKEISERLGHSRISI
                     TMDTYSHVTDKMRNETVDIMENLRKNS"
BASE COUNT          402 a          192 c          214 g          297 t
ORIGIN      
        1 agatatcaat gatgatggct caagaaaaaa agtggagaaa gttggcggag acacccgacc
       61 agaggccgaa gctgctttac gaaaagtttt atcagatatt gacgaaacag gacaatactt
      121 tttaggtacg gatactcgag taaaacaata ccttgatttt tggatggagg aatacgttaa
      181 actaaatcta aaatacaata cctatgaaaa ctaccgattt accatcaaaa atcatataaa
      241 cggttattta ggaaagaaaa aacttacgga tctctcccct gctcttttac aaaatttcat
      301 caatgctgaa tttaaaaagg gttactcgaa gaaaacaatg actattactc actctgtcct
      361 taagaatgcg ctgaatatgg cggtttatcc ttggggatta atcaagcaaa atcctatgct
      421 gtatgtaaaa ataccaaaat atgaagcacg accaacgact aaaaaagatc taaaaatcat
      481 ttctcttgag gactttgatc atatgctaga aattactcct gaaggacatc ctttctatat
      541 tcctttgaat attggatttt atacgggaat gcgcgttggc gaagtttgtg gtctgacgtg
      601 ggatgatgtc gatttttcaa atggaacaat tactgtagag aaacaaatgg taaagaatga
      661 cggcgcatgg gtatatggta cgccaaagac aagcagttcc aatcgaacga tttttattgg
      721 acaaaccttg ctagcaattc tgaaaaaaca taagaaacaa caattagaaa atcgaatgaa
      781 gtatggaaag ctctacattg attcaaatgc agtatgtacg aaggaagacg gtgagctagt
      841 tacgccaagt gtagtgaaat ggaatactag gaggatatcg aatgcactct ccctctcttt
      901 taacttccat tctctcagac atactcatgc tacacttctt ctcgaaaatg gcgcaaaaat
      961 gaaagaaatc tctgaacgat tgggccacag cagaatttca attacgatgg atacttactc
     1021 gcatgtaaca gataagatga gaaatgaaac ggtcgatatc atggaaaatc ttagaaagaa
     1081 ttcttgattt ttgccaccga aaaaa
//
LOCUS       DARBET010000200         1103 bp    DNA     linear   BCT 07-NOV-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4027
            SAMEA114569841-rid20112183.denovo.207, whole genome shotgun
            sequence.
ACCESSION   DARBET010000200 DARBET010000000
VERSION     DARBET010000200.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA114569841
            Sequence Read Archive: ERR12203666
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1103)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1103)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001972618.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001972618.1
            Genome Coverage       :: 132x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/07/2023 13:18:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,041
            CDSs (total)                      :: 2,975
            Genes (coding)                    :: 2,908
            CDSs (with protein)               :: 2,908
            Genes (RNA)                       :: 66
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 67
            CDSs (without protein)            :: 67
            Pseudo Genes (ambiguous residues) :: 0 of 67
            Pseudo Genes (frameshifted)       :: 26 of 67
            Pseudo Genes (incomplete)         :: 28 of 67
            Pseudo Genes (internal stop)      :: 20 of 67
            Pseudo Genes (multiple problems)  :: 6 of 67
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1103
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA114569841-rid20112183.denovo.207"
                     /isolate="VRE4027"
                     /db_xref="taxon:1352"
                     /country="Denmark"
     gene            complement(1..634)
                     /locus_tag="SCJ86_002933"
                     /pseudo
     CDS             complement(1..634)
                     /locus_tag="SCJ86_002933"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611665.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ISL3 family transposase"
BASE COUNT          341 a          190 c          189 g          383 t
ORIGIN      
        1 aggtaaaaaa taagtctgat tccttgtctc taaaggcttc ggtcagctca tggaaaaaag
       61 tataggcgtc ctttaaaggc gttgaaaatg ccagcaatcg atcaatcatc attgcttcag
      121 tgagtagcgg gtatttaggt gcacgaaaac tgcgccatgt tttgtaatca tagtgattga
      181 ttttttgacg gttttttaga agaaagcgcc agttcttttt cactttctcg gcttcccgtc
      241 gttgccctaa ctggtgtaat cttttcattt cttgtacacg aaaatcattg aacgcctgat
      301 tcaaatgttt gatgatatga aaacaatcaa tgacgacttt cgcatttggt aagacacgtt
      361 ttgtcagttg gaagtaggcg gcgttcatat ctgtcactaa atattccacc gcttcaggat
      421 ttgcacagcg actaaaatag tgtgttaatc gagacaattt tcgtgtaggc aaaatatcaa
      481 tgagttttcc agtctcgcca tccgcacaaa taaagctcat tttatccaca attgaagcgt
      541 gcgaacgaaa ttcatccacc atcaatactt ttggcagggt cctttttgac ggcttcggaa
      601 gatagttttt caactctttc aatgtacgaa tcatatacct aaaataacaa tgggactgaa
      661 gaatcaattt tgattcatca gtcccataaa ttatagagcc tatattttgc gaattgcgct
      721 acggtttctt cttttctttt gtctgtaata tgaatatata gatccataat gatttgaata
      781 gaagagtggc ctaaacgctc ttgcacatct ttaatatttg cactagcttg ctaaaagcaa
      841 gctagcatgt gtgtgtctaa gaccatgaat agtaattcgt ttaagattgt ttttcctgat
      901 gattacatct aaccattttc gagatttaga taattgaagg tgttcatttc tttctggtaa
      961 aaatttaaat atataactac attttctcta ctaactttga aacactactt ttgtaatata
     1021 aaaaatattt tattaataat aaaattaatc acattttata tagtgagtaa tctagtgttt
     1081 taaaaaaata aatgatacta tat
//
LOCUS       DARBET010000201         1076 bp    DNA     linear   BCT 07-NOV-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4027
            SAMEA114569841-rid20112183.denovo.208, whole genome shotgun
            sequence.
ACCESSION   DARBET010000201 DARBET010000000
VERSION     DARBET010000201.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA114569841
            Sequence Read Archive: ERR12203666
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1076)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1076)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001972618.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001972618.1
            Genome Coverage       :: 132x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/07/2023 13:18:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,041
            CDSs (total)                      :: 2,975
            Genes (coding)                    :: 2,908
            CDSs (with protein)               :: 2,908
            Genes (RNA)                       :: 66
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 67
            CDSs (without protein)            :: 67
            Pseudo Genes (ambiguous residues) :: 0 of 67
            Pseudo Genes (frameshifted)       :: 26 of 67
            Pseudo Genes (incomplete)         :: 28 of 67
            Pseudo Genes (internal stop)      :: 20 of 67
            Pseudo Genes (multiple problems)  :: 6 of 67
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1076
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA114569841-rid20112183.denovo.208"
                     /isolate="VRE4027"
                     /db_xref="taxon:1352"
                     /country="Denmark"
     gene            247..>1076
                     /locus_tag="SCJ86_002934"
     CDS             247..>1076
                     /locus_tag="SCJ86_002934"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287843.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS256 family transposase"
                     /protein_id="HEG1170407.1"
                     /translation="MPNFTTEIMETLINKGDLDELFRCHLELAVNSLLQAELTAFLDY
                     EKYDRAGFNSGNSRNGNYSRSFRTEYGELNLVIPRDRNGKFSQQTLPAYKRTNDSLET
                     TIIQLFQKGITMSEISELIEKMYGHHYTPQTISNMTKIVSEDIIAFKERSLESRYSVI
                     FMDATHIPLKRQTVSKEAVYIVIGIRLDGTKEVLGFSLAPTESAYVWKEILQDLKDRG
                     LKEVLLVVTDGLSGINDSIHSVYPNAQFQQCCVHISRNIAHKVRVSDRQEICSDFKLV
                     "
BASE COUNT          377 a          152 c          183 g          364 t
ORIGIN      
        1 agttattctt ttattactat gtttgggttt tatagcaaag aaagtttaat aagagtttgg
       61 acctagcaaa caatattaat gaggtcatta aatcttatta tctttagtga tataacgaaa
      121 tatctacaca gacaaaatga tagcgtaaaa tattttgtgt aaatagatat ttagggtaga
      181 aaaaaagaaa gtccattctg taaaattaaa gttaccacaa ccgaaatttt ataggaggac
      241 tttctcatgc ccaattttac tacagaaatt atggaaacac taatcaataa aggtgattta
      301 gatgaattat ttcgatgtca tttagaatta gctgttaatt cattattgca ggctgaatta
      361 acagcttttc ttgattatga aaaatatgat cgtgctgggt tcaattcagg taattcccgt
      421 aatgggaatt attcacgttc attcagaaca gagtatggag aattaaattt ggtaattcct
      481 agagatagaa atggtaagtt ttctcaacaa actttacctg cttataaaag aaccaatgac
      541 tctttagaaa ccactattat tcagctattt caaaaaggaa ttactatgtc tgaaatctct
      601 gaattgattg aaaaaatgta cgggcatcac tatacgccac aaaccatttc aaatatgact
      661 aaaatagttt ctgaagatat tattgccttt aaagaaaggt ccttagaatc cagatactct
      721 gtcattttta tggatgctac tcacattcct ttaaaaagac aaactgtttc aaaagaagca
      781 gtttatattg taattggcat tcgattagat ggaaccaaag aggttcttgg gtttagtctt
      841 gctccaactg aatctgctta tgtttggaaa gagattcttc aagacctaaa agaccgtggt
      901 ttaaaagagg ttttattagt cgtaactgat ggtttaagtg gcattaacga tagtattcat
      961 agtgtttatc caaatgctca atttcaacaa tgttgtgtcc atatatctag aaatatcgct
     1021 cataaggttc gtgttagtga tcgacaagaa atctgtagcg atttcaaatt agttta
//
LOCUS       DARBET010000202         1059 bp    DNA     linear   BCT 07-NOV-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4027
            SAMEA114569841-rid20112183.denovo.209, whole genome shotgun
            sequence.
ACCESSION   DARBET010000202 DARBET010000000
VERSION     DARBET010000202.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA114569841
            Sequence Read Archive: ERR12203666
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1059)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1059)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001972618.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001972618.1
            Genome Coverage       :: 132x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/07/2023 13:18:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,041
            CDSs (total)                      :: 2,975
            Genes (coding)                    :: 2,908
            CDSs (with protein)               :: 2,908
            Genes (RNA)                       :: 66
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 67
            CDSs (without protein)            :: 67
            Pseudo Genes (ambiguous residues) :: 0 of 67
            Pseudo Genes (frameshifted)       :: 26 of 67
            Pseudo Genes (incomplete)         :: 28 of 67
            Pseudo Genes (internal stop)      :: 20 of 67
            Pseudo Genes (multiple problems)  :: 6 of 67
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1059
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA114569841-rid20112183.denovo.209"
                     /isolate="VRE4027"
                     /db_xref="taxon:1352"
                     /country="Denmark"
     gene            complement(37..993)
                     /locus_tag="SCJ86_002935"
     CDS             complement(37..993)
                     /locus_tag="SCJ86_002935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326835.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEG1170408.1"
                     /translation="MELYTLPIIIKTEKMLKDLKQYYPLSAYEKKKLFHFLAINLLAE
                     QADSLDIASNQSLFFSDIGFYHLKGVLGEKSVIFINKTLSWLYLHDKLDQKYLRKNED
                     ETIEWLNERFIESFENQFNLLREETSQLIRRELTKIHFKLLHYTINKADEFPLDVSFF
                     RENYPAFYCYLIEYINDLTSKRKELLQDKFFLFFSYLLLLINHIPIQLISEPVKIIID
                     FSYGAAYNQFIKKNLSLYINLNTEVIEAGKPEFPDVIITNRNNLYEESASQVVVWLDP
                     PRAVDWGNLTKILLTIQEEKYKDSKIDKQIDDFPEEIIDRIE"
BASE COUNT          344 a          176 c          160 g          379 t
ORIGIN      
        1 ttagaaaaag cagtcggact ttctaaaaga agttcactat tcgatcctgt cgatgatttc
       61 ttcaggaaaa tcgtctattt gcttgtcaat tttgctgtct ttgtatttct cttcttggat
      121 agtcaacaaa atctttgtaa gattccccca gtctactgct cttggcggat cgagccaaac
      181 cactacttga gatgcacttt cttcatatag attattccta ttcgtgatta taacatccgg
      241 gaactcaggc tttcctgcct ctatcacttc cgtgttcaga ttaatgtaca agcttaaatt
      301 tttcttgata aattgattat aggcagcacc ataagaaaaa tcgatgatga tctttactgg
      361 ttcagaaatc agttgaatgg gtatgtgatt gatcaataat aataaataac tgaaaaaaag
      421 aaaaaattta tcttgaagca actctttgcg ttttgacgtt agatcattta tatactctat
      481 caaatagcaa taaaatgctg gatagttttc tctaaaaaaa gaaacatcaa gtggaaattc
      541 atcagcttta ttaatagtgt aatgcaaaag tttaaaatgt attttggtta actctcttcg
      601 gatcaattgg ctcgtctctt ctcgcaacag attaaactga ttttcaaaac tttcaatgaa
      661 tcgttcattc aaccattcga ttgtttcatc ttcatttttt ctcaagtatt tttgatccaa
      721 tttatcatga agataaagcc aagagagtgt cttattgata aaaataacgg atttttctcc
      781 taatactcct tttaagtggt aaaatcctat atccgaaaaa aatagcgact gattgctagc
      841 aatatctaaa gaatcagctt gttcagccaa caggttgatt gctaaaaagt ggaataattt
      901 ctttttttca taagcagaca gaggataata ctgttttaaa tcttttaaca ttttttctgt
      961 ttttataatt ataggtagcg tatacaattc cacaaaactt atctctgttg agttaaaaag
     1021 attatataaa aaaagccgaa tgtttttagg attccctgt
//
LOCUS       DARBET010000203         1051 bp    DNA     linear   BCT 07-NOV-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4027
            SAMEA114569841-rid20112183.denovo.210, whole genome shotgun
            sequence.
ACCESSION   DARBET010000203 DARBET010000000
VERSION     DARBET010000203.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA114569841
            Sequence Read Archive: ERR12203666
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1051)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1051)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001972618.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001972618.1
            Genome Coverage       :: 132x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/07/2023 13:18:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,041
            CDSs (total)                      :: 2,975
            Genes (coding)                    :: 2,908
            CDSs (with protein)               :: 2,908
            Genes (RNA)                       :: 66
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 67
            CDSs (without protein)            :: 67
            Pseudo Genes (ambiguous residues) :: 0 of 67
            Pseudo Genes (frameshifted)       :: 26 of 67
            Pseudo Genes (incomplete)         :: 28 of 67
            Pseudo Genes (internal stop)      :: 20 of 67
            Pseudo Genes (multiple problems)  :: 6 of 67
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1051
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA114569841-rid20112183.denovo.210"
                     /isolate="VRE4027"
                     /db_xref="taxon:1352"
                     /country="Denmark"
     gene            6..980
                     /gene="bsh"
                     /locus_tag="SCJ86_002936"
     CDS             6..980
                     /gene="bsh"
                     /locus_tag="SCJ86_002936"
                     /EC_number="3.5.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342103.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="choloylglycine hydrolase"
                     /protein_id="HEG1170409.1"
                     /translation="MCTSITYVTSDHYFGRNFDYEISYNEVVTVTPRNYKLNFRKVND
                     LDTHYAMIGIAAGIADYPLYYDATNEKGLSMAGLNFSGYADYKEIQEGKDNVSPFEFI
                     PWILGQCSTVGEAKKLLKNINLANINYSDELPLSPLHWLLADKEKSIVIESMKDGLHI
                     YDNPVGVLTNNPSFDYQLFNLNNYRVLSSETPKNNFSNQISLNAYSRGMGGIGLPGDL
                     SSVSRFVKATFTKLNSVSGDSESESISQFFHILGSVEQQKGLCDVGDGKYEYTIYSSC
                     CNVDKGIYYYRTYEDSQITAIDMNKEDLDSHKLISYPIIEKQQIKYIN"
BASE COUNT          377 a          132 c          182 g          360 t
ORIGIN      
        1 aaactatgtg tacgtctatt acttatgtaa caagtgatca ttattttgga aggaattttg
       61 attatgaaat atcttacaat gaagtagtta ctgttactcc aagaaattat aagttgaatt
      121 ttcgaaaggt aaatgatttg gatactcatt atgcaatgat tggtattgcc gctggtatag
      181 ctgactaccc tctttattac gatgcgacaa atgaaaaagg attgagtatg gctgggctaa
      241 atttttctgg gtatgctgat tataaagaaa tacaagaagg gaaagacaat gtatctcctt
      301 ttgaatttat tccttggatt ttaggacaat gctcaacagt aggagaagct aaaaaattgt
      361 taaaaaatat caatttagca aatataaatt atagtgacga acttccttta tcccctttac
      421 attggctatt agctgataaa gaaaaatcaa ttgtcattga aagtatgaaa gatggacttc
      481 atatatatga taaccctgtg ggcgttctta ccaataatcc ttcatttgac tatcaattat
      541 ttaatttaaa caattatcgt gtcttatcga gtgaaactcc taaaaataat ttttcaaatc
      601 aaataagttt gaatgcctat agccgcggta tgggagggat aggcttgcct ggagatttat
      661 cctcagtatc tcgttttgtt aaagcgactt ttacgaagct gaattctgta tctggagatt
      721 cagagtcaga aagtattagt caatttttcc atatcttagg ttcagtagaa caacaaaaag
      781 gtttgtgtga tgtaggtgat ggaaaatatg aatatacaat ttattcttct tgttgcaatg
      841 ttgacaaagg aatctattat tatcgaacat atgaagacag tcaaattact gcaattgata
      901 tgaataaaga agacttagat agtcataagt taattagtta tccaattata gaaaaacaac
      961 aaattaaata tataaattag ttaatgtgtt gtgattgatt atttaatata gatataatac
     1021 aaaaaggcaa agatattttc aaaacatttt t
//
LOCUS       DARBET010000204         1036 bp    DNA     linear   BCT 07-NOV-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4027
            SAMEA114569841-rid20112183.denovo.211, whole genome shotgun
            sequence.
ACCESSION   DARBET010000204 DARBET010000000
VERSION     DARBET010000204.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA114569841
            Sequence Read Archive: ERR12203666
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1036)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1036)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001972618.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001972618.1
            Genome Coverage       :: 132x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/07/2023 13:18:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,041
            CDSs (total)                      :: 2,975
            Genes (coding)                    :: 2,908
            CDSs (with protein)               :: 2,908
            Genes (RNA)                       :: 66
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 67
            CDSs (without protein)            :: 67
            Pseudo Genes (ambiguous residues) :: 0 of 67
            Pseudo Genes (frameshifted)       :: 26 of 67
            Pseudo Genes (incomplete)         :: 28 of 67
            Pseudo Genes (internal stop)      :: 20 of 67
            Pseudo Genes (multiple problems)  :: 6 of 67
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1036
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA114569841-rid20112183.denovo.211"
                     /isolate="VRE4027"
                     /db_xref="taxon:1352"
                     /country="Denmark"
     gene            1..495
                     /locus_tag="SCJ86_002937"
                     /pseudo
     CDS             1..495
                     /locus_tag="SCJ86_002937"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000221326.1"
                     /note="frameshifted; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=2
                     /transl_table=11
                     /product="IS30 family transposase"
     gene            818..934
                     /locus_tag="SCJ86_002938"
     CDS             818..934
                     /locus_tag="SCJ86_002938"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010768364.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1972 domain-containing protein"
                     /protein_id="HEG1170410.1"
                     /translation="MIGLNAISAKYCKFKAFVEKFTERQTDKIIQYHITYTD"
BASE COUNT          392 a          145 c          154 g          345 t
ORIGIN      
        1 tgagtttggt caccttgaag gtgatacgat cgttggcatt catcataaaa gtgccgtcat
       61 tactttagtt gaaagattat ctaaagtcat tatcacgatt aaacccaacg gccgtaaggc
      121 attagatatt gaaactgccc ttaatcaatg gttttctcgc ttccctaaaa acttctttaa
      181 atctattacg ctattacgtt tgactgtgga aaagaatttt ctaactggaa agccattagt
      241 aaccaacatg atattgatat atattttgcg gaccctggaa caccttctca acgcccatta
      301 aacgagaatt ctaacgggat tctgcgtcgt aatggactgc cgaaatcaat ggattttaga
      361 gaagtgaatc agacatttat ttccagtgtc agcaatcaac gtaatcatat tccaagaaaa
      421 tcattgaatt acagaacacc aatcgaaata tttttgagct atgtacaaga agcattttat
      481 tctagcttaa tttgacaaat cataaataat aaagaattga acttctgaga aaacaaagaa
      541 tcttttttct ttttttgaat tgactcatgg attggcgtta aaattttatt ttgaaaaagt
      601 tatttctggt ttaactcaaa aaaagagata tgaatctaat agtgatagaa aggatgttat
      661 ttttcatgaa atggttgttt tagatttaat acatataact aatgaatgct attgaatgta
      721 tttaaactaa ttaacaaaag aatttgaatt gttaataaaa tccaataaaa tatattaata
      781 aagtagatag aaataaaaaa acaaagacta aacatatata attggattaa acgcaatatc
      841 tgcaaaatat tgtaaattta aagcctttgt agaaaaattt actgaaagac aaactgataa
      901 gattattcag tatcatatta cctatactga ttaaaatttt ttgaaacggg atactaaata
      961 aaaaatttat ttataacaga actaattatt taatgcggat gcgccaatac ttggtcctgc
     1021 aagagctatt tatttt
//
LOCUS       DARBET010000205         1014 bp    DNA     linear   BCT 07-NOV-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4027
            SAMEA114569841-rid20112183.denovo.212, whole genome shotgun
            sequence.
ACCESSION   DARBET010000205 DARBET010000000
VERSION     DARBET010000205.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA114569841
            Sequence Read Archive: ERR12203666
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1014)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1014)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001972618.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001972618.1
            Genome Coverage       :: 132x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/07/2023 13:18:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,041
            CDSs (total)                      :: 2,975
            Genes (coding)                    :: 2,908
            CDSs (with protein)               :: 2,908
            Genes (RNA)                       :: 66
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 67
            CDSs (without protein)            :: 67
            Pseudo Genes (ambiguous residues) :: 0 of 67
            Pseudo Genes (frameshifted)       :: 26 of 67
            Pseudo Genes (incomplete)         :: 28 of 67
            Pseudo Genes (internal stop)      :: 20 of 67
            Pseudo Genes (multiple problems)  :: 6 of 67
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1014
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA114569841-rid20112183.denovo.212"
                     /isolate="VRE4027"
                     /db_xref="taxon:1352"
                     /country="Denmark"
     gene            <1..578
                     /locus_tag="SCJ86_002939"
     CDS             <1..578
                     /locus_tag="SCJ86_002939"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177096.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="oxidoreductase"
                     /protein_id="HEG1170411.1"
                     /translation="AAPLRYKNIMEVVRNSIQSKKVYSVRAICSTYLPDWRKGTDYRN
                     NYSAFKSMGGGVELDCIHELDYIINLFGFPKSVKKQFGKYSNLEINSNDLATYILRYD
                     DKIIEIHLDYFGRVPTRKFQLFTLDECINVDLLNNKISFEENIVKEMPESANDMYEKE
                     LKYFFENVVTGKENWNDLYHANEVLKIAEEK"
     gene            579..>1014
                     /locus_tag="SCJ86_002940"
     CDS             579..>1014
                     /locus_tag="SCJ86_002940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289370.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acylneuraminate cytidylyltransferase family
                     protein"
                     /protein_id="HEG1170412.1"
                     /translation="MEILFTICGRAGSKGFKNKNLKYLAGKPLVHFALAMIDIYQQKH
                     PEHNCTTCLNTDSNELVELTTKLNPSVKIVERKPELATDTVGKIEVIRDSFLQLERED
                     NIFDLIIDLDLTSPLREVSNLEELIDEHLKNLDKDVYFSVVSA"
BASE COUNT          383 a          113 c          182 g          336 t
ORIGIN      
        1 ttgctgctcc actgaggtat aaaaatatca tggaagttgt tcgtaatagt attcaaagta
       61 aaaaagtata ttctgtaaga gctatttgtt ctacttatct tcctgattgg agaaaaggta
      121 ctgattatag aaataattat tctgccttta aatctatggg gggaggagta gaattagact
      181 gtatacatga attggattat attataaacc tatttggttt ccctaaatca gtaaaaaaac
      241 agtttggtaa atattcgaat cttgaaatta acagtaatga tttagcaaca tatatattga
      301 gatacgatga taaaattata gaaattcatt tagattattt tggaagagtt cctacaagaa
      361 aatttcaatt gtttacttta gatgaatgta tcaatgttga cttattaaat aataaaataa
      421 gttttgaaga aaacattgtt aaagaaatgc ctgaatctgc taatgatatg tatgagaagg
      481 agttgaaata tttctttgaa aacgtagtaa caggcaaaga aaactggaat gatttatatc
      541 atgcaaacga agtattgaaa atagctgagg agaaataaat ggaaatttta tttactattt
      601 gtggtagagc tgggtctaag gggtttaaaa ataaaaattt gaaatatttg gcaggaaagc
      661 ccctagtcca tttcgcttta gcaatgatag atatctatca acaaaaacat cctgaacata
      721 attgcacgac ttgtttgaat acggacagta atgagttagt agaattgacg actaagttaa
      781 atccgtctgt gaaaattgta gaacgtaaac ctgaattagc tacagatact gtagggaaaa
      841 ttgaagtgat acgtgattca ttcttacaac tagaaagaga agataatata tttgatttaa
      901 ttatagattt agatttgact tcacctttac gtgaagtttc taatcttgaa gaattaatag
      961 atgaacatct aaaaaattta gataaagacg tctacttttc agttgtttct gcaa
//
LOCUS       DARBET010000206          997 bp    DNA     linear   BCT 07-NOV-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4027
            SAMEA114569841-rid20112183.denovo.213, whole genome shotgun
            sequence.
ACCESSION   DARBET010000206 DARBET010000000
VERSION     DARBET010000206.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA114569841
            Sequence Read Archive: ERR12203666
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 997)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 997)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001972618.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001972618.1
            Genome Coverage       :: 132x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/07/2023 13:18:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,041
            CDSs (total)                      :: 2,975
            Genes (coding)                    :: 2,908
            CDSs (with protein)               :: 2,908
            Genes (RNA)                       :: 66
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 67
            CDSs (without protein)            :: 67
            Pseudo Genes (ambiguous residues) :: 0 of 67
            Pseudo Genes (frameshifted)       :: 26 of 67
            Pseudo Genes (incomplete)         :: 28 of 67
            Pseudo Genes (internal stop)      :: 20 of 67
            Pseudo Genes (multiple problems)  :: 6 of 67
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..997
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA114569841-rid20112183.denovo.213"
                     /isolate="VRE4027"
                     /db_xref="taxon:1352"
                     /country="Denmark"
     gene            complement(26..913)
                     /locus_tag="SCJ86_002941"
     CDS             complement(26..913)
                     /locus_tag="SCJ86_002941"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016627954.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucosaminidase domain-containing protein"
                     /protein_id="HEG1170413.1"
                     /translation="MQELKTRSARHHRPKRKMVINKKAIAPLIGTSLLVGPMMVTAVP
                     QHVHADEQGSQTVADPQAFIDQIGWSASEVAASNDLYASVMIAQALLESAYGTSGLAS
                     APNYNLFGVKGSYNGQVVYMATKEYVNGQWVTMNEPFRKYSSYWESFQDHANVLRSTS
                     FSTGDYHYAGVWKSNTTSFYDATAYLTGRYATDPNYGAKLNSLINTYNLTRFDTPSTG
                     SATTTTSTTTTTTTTTSTSSSSTTDVNTNNGQTYTVVSGDTLWDIAQKFGMSVDDLMA
                     KNGMSMSSYSLLVGQELNV"
BASE COUNT          288 a          207 c          179 g          323 t
ORIGIN      
        1 taatttcttt gcctttttat ctaaatcaaa cgtttagttc ttgaccaact aataaagaat
       61 aactggacat agacatgcca tttttcgcca ttagatcatc aacagacatg ccaaattttt
      121 gtgcaatatc ccatagcgta tcacctgaca cgactgtata tgtctgacca ttatttgtat
      181 ttacatctgt tgttgaagat gatgacgttg aagtcgtcgt agtagtcgtt gttgttgtac
      241 ttgtggttgt tgtagccgaa cctgttgatg gcgtatcaaa acgggttaaa ttataagtat
      301 taatcaatga gttcaatttg gccccataat ttggatcggt agcataacga cctgtcaagt
      361 acgctgttgc atcataaaaa cttgttgtat tgcttttcca cacgccagca taatgataat
      421 cgcccgtgga aaaactggtt gaacgaagaa cattcgcatg gtcttggaag gattcccaat
      481 aagaagaata ttttcggaat ggctcattca tcgttaccca ttgtccgttt acatattctt
      541 tggttgccat ataaacgacc tgtccgttat agcttccttt cacaccgaat agattataat
      601 ttggcgcact agctaatccg gaagtgccat atgcactttc caacaacgct tgagcgatca
      661 tgactgaagc atacaagtca ttacttgcag caacttctga cgcagaccag ccgatttgat
      721 cgataaacgc ttgaggatca gcgactgttt gagatccttg ttcatctgca tgaacatgtt
      781 gcggtaccgc tgtaaccatc atcggcccaa ctaaaagact cgttccaatt aaaggagcaa
      841 tcgctttttt attgatcacc atttttcttt taggtctatg gtgccgggca ctgcgggtct
      901 tcagctcttg cattcaaatt cctcctcaaa ataaaaaaac ttaataaaaa gttctcttct
      961 taaaacaaac ttaattaatt aaattatact tttaatt
//
LOCUS       DARBET010000207          994 bp    DNA     linear   BCT 07-NOV-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4027
            SAMEA114569841-rid20112183.denovo.214, whole genome shotgun
            sequence.
ACCESSION   DARBET010000207 DARBET010000000
VERSION     DARBET010000207.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA114569841
            Sequence Read Archive: ERR12203666
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 994)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 994)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001972618.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001972618.1
            Genome Coverage       :: 132x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/07/2023 13:18:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,041
            CDSs (total)                      :: 2,975
            Genes (coding)                    :: 2,908
            CDSs (with protein)               :: 2,908
            Genes (RNA)                       :: 66
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 67
            CDSs (without protein)            :: 67
            Pseudo Genes (ambiguous residues) :: 0 of 67
            Pseudo Genes (frameshifted)       :: 26 of 67
            Pseudo Genes (incomplete)         :: 28 of 67
            Pseudo Genes (internal stop)      :: 20 of 67
            Pseudo Genes (multiple problems)  :: 6 of 67
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..994
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA114569841-rid20112183.denovo.214"
                     /isolate="VRE4027"
                     /db_xref="taxon:1352"
                     /country="Denmark"
     gene            complement(<1..>994)
                     /locus_tag="SCJ86_002942"
     rRNA            complement(<1..>994)
                     /locus_tag="SCJ86_002942"
                     /product="16S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00177"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00177"
BASE COUNT          209 a          307 c          225 g          253 t
ORIGIN      
        1 agcacgtgtg tagcccaggt cataaggggc atgatgattt gacgtcatcc ccaccttcct
       61 ccggtttgtc accggcagtc ttgctagagt gcccaactga atgatggcaa ctaacaataa
      121 gggttgcgct cgttgcggga cttaacccaa catctcacga cacgagctga cgacaaccat
      181 gcaccacctg tcactttgcc cccgaagggg aagctctatc tctagagtgg tcaaaggatg
      241 tcaagacctg gtaaggttct tcgcgttgct tcgaattaaa ccacatgctc caccgcttgt
      301 gcgggccccc gtcaattcct ttgagtttca accttgcggt cgtactcccc aggcggagtg
      361 cttaatgcgt tagctgcagc actgaagggc ggaaaccctc caacacttag cactcatcgt
      421 ttacggcgtg gactaccagg gtatctaatc ctgtttgctc cccacgcttt cgagcctcag
      481 cgtcagttac agaccagaga gccgccttcg ccactggtgt tcctccatat atctacgcat
      541 ttcaccgcta cacatggaat tccactctcc tcttctgcac tcaagtctcc cagtttccaa
      601 tgaccctccc cggttgagcc gggggctttc acatcagact taagaaaccg cctgcgctcg
      661 ctttacgccc aataaatccg gacaacgctt gccacctacg tattaccgcg gctgctggca
      721 cgtagttagc cgtggctttc tggttagata ccgtcaaggg atgaacagtt actctcatcc
      781 ttgttcttct ctaacaacag agttttacga tccgaaaacc ttcttcactc acgcggcgtt
      841 gctcggtcag actttcgtcc attgccgaag attccctact gctgcctccc gtaggagttt
      901 gggccgtgtc tcagtcccaa tgtggccgat caccctctca ggtcggctat gcatcgtggc
      961 cttggtgagc cgttacctca ccaactagct aatg
//
LOCUS       DARBET010000208          931 bp    DNA     linear   BCT 07-NOV-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4027
            SAMEA114569841-rid20112183.denovo.215, whole genome shotgun
            sequence.
ACCESSION   DARBET010000208 DARBET010000000
VERSION     DARBET010000208.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA114569841
            Sequence Read Archive: ERR12203666
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 931)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 931)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001972618.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001972618.1
            Genome Coverage       :: 132x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/07/2023 13:18:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,041
            CDSs (total)                      :: 2,975
            Genes (coding)                    :: 2,908
            CDSs (with protein)               :: 2,908
            Genes (RNA)                       :: 66
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 67
            CDSs (without protein)            :: 67
            Pseudo Genes (ambiguous residues) :: 0 of 67
            Pseudo Genes (frameshifted)       :: 26 of 67
            Pseudo Genes (incomplete)         :: 28 of 67
            Pseudo Genes (internal stop)      :: 20 of 67
            Pseudo Genes (multiple problems)  :: 6 of 67
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..931
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA114569841-rid20112183.denovo.215"
                     /isolate="VRE4027"
                     /db_xref="taxon:1352"
                     /country="Denmark"
     gene            <1..>931
                     /locus_tag="SCJ86_002943"
     CDS             <1..>931
                     /locus_tag="SCJ86_002943"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_086557923.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="ISL3 family transposase"
                     /protein_id="HEG1170414.1"
                     /translation="HCFISQKVRWSVIARLKENTSMTEIARQKNISTSSVYRVMKRFY
                     RPLNPFKQTLPKVLCFDEFKSVRSVASAMSFIMMDGQSHALLDIVENRRLPYLERYFS
                     RFSLATREAVQLIVIDMYAPYVSLVKKLFPNAQLIIDRFHIVQHIGRTFLNHRVKETT
                     VRLKGNSHSQRGLGKKLKRYWKLLQKEEAKLNYEKRVWRASFKDYLTESEIADRLLAA
                     CPNLRQGYQLYQDILYAVKKRDQSLFEDCLTRDISGLPETYTTTLRTFKKFLPQIQNA
                     LHYSYSNGPLECLNNHIKVLKRNAYGFRNFYNFK"
BASE COUNT          286 a          190 c          180 g          275 t
ORIGIN      
        1 acactgtttc attagtcaaa aagttcgttg gtctgtcatt gctcgcttaa aagagaatac
       61 ttccatgacg gaaatcgctc gtcagaaaaa tatttccact tcttcggtct atcgtgtcat
      121 gaagcgattc taccgcccat tgaatccttt caaacagact ttgccaaagg tcttatgctt
      181 tgatgaattc aaatcggttc gaagtgttgc cagtgcaatg agttttatca tgatggacgg
      241 acaaagccat gcattattgg atattgtcga aaatcgacgc ttgccttatt tagaacgcta
      301 tttttctcgg ttttcattag cgactcgtga agccgttcaa ttgattgtta ttgatatgta
      361 tgcgccttat gtttcgttgg tgaaaaagct tttcccgaat gctcagttga tcatcgatcg
      421 tttccacatc gtccaacata ttggcagaac tttcttgaat catcgagtga aagaaaccac
      481 ggtacgactt aaaggaaatt cccactctca acgagggtta gggaaaaaat tgaagcgcta
      541 ttggaaactc ctacaaaaag aagaagcaaa gttaaactat gaaaaacggg tttggcgtgc
      601 cagttttaaa gattatttga ctgaaagtga gattgccgat cgactgttag ctgcttgtcc
      661 caacttacgc caaggctatc agttatacca agacattctt tatgcggtaa agaaaagaga
      721 tcagtcacta tttgaggatt gtttaactag agacattagc ggtctgccag aaacctatac
      781 aaccacttta cgaacgttta aaaagtttct gccacagatc caaaatgcgc ttcactacag
      841 ctattcaaac ggaccattgg aatgcttaaa caaccacatc aaagtattaa aacgcaatgc
      901 gtacggcttt cgcaactttt acaatttcaa a
//
LOCUS       DARBET010000209          918 bp    DNA     linear   BCT 07-NOV-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4027
            SAMEA114569841-rid20112183.denovo.216, whole genome shotgun
            sequence.
ACCESSION   DARBET010000209 DARBET010000000
VERSION     DARBET010000209.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA114569841
            Sequence Read Archive: ERR12203666
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 918)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 918)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001972618.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001972618.1
            Genome Coverage       :: 132x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/07/2023 13:18:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,041
            CDSs (total)                      :: 2,975
            Genes (coding)                    :: 2,908
            CDSs (with protein)               :: 2,908
            Genes (RNA)                       :: 66
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 67
            CDSs (without protein)            :: 67
            Pseudo Genes (ambiguous residues) :: 0 of 67
            Pseudo Genes (frameshifted)       :: 26 of 67
            Pseudo Genes (incomplete)         :: 28 of 67
            Pseudo Genes (internal stop)      :: 20 of 67
            Pseudo Genes (multiple problems)  :: 6 of 67
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..918
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA114569841-rid20112183.denovo.216"
                     /isolate="VRE4027"
                     /db_xref="taxon:1352"
                     /country="Denmark"
     gene            complement(210..473)
                     /locus_tag="SCJ86_002944"
     CDS             complement(210..473)
                     /locus_tag="SCJ86_002944"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347584.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEG1170415.1"
                     /translation="MFIKYGADTVATMFTEEYQVDYYEVIDKCVHFVYEKVWNFMNFA
                     ISEEILRVQENRTGIFTRDRKLNLIAHYIRNRWSSIPNFNYKK"
BASE COUNT          288 a          153 c          105 g          372 t
ORIGIN      
        1 taaaaaaact ggtagtacaa tcttttaaat tctcattgtt ttgtacgttc tttactatca
       61 aaaaaacaaa aaaaacgcgt ttctacgcgt gaccaaaatt tttagctcta taatttctaa
      121 gactgataga aaatcattct gtttttgaag tcttttttca taaatactac ttgatttatt
      181 gtgtatattg tataaaagat tgttcataat tattttttat aattaaaatt tggtattgat
      241 gaccagcgat tccgtatata atgcgcaatc aaattaagct ttctgtctct ggtaaatatt
      301 cctgttctgt tttcttgtac tcgtaagatt tcttcactta tcgcaaaatt cataaaattc
      361 cacacttttt cataaacaaa atgtacacat ttatctatta cttcataata gtctacttga
      421 tattcttctg taaacatggt tgctactgta tctgcaccat atttgataaa catttgggct
      481 actcccttaa attgagaaaa gcccattagt ttatctacta atacttctaa aatttcttgc
      541 tttcttcttt aacatcgtcc cgtaaggaat catgtagtat gctggaggaa gttcattttc
      601 taaataattg tatcgcttca attgctcaaa ggctattctc tacaattaat tacaccaatt
      661 actttatctt cacttatata agatttctaa acatttttcg tcaaatgaag agaaaatatt
      721 tttttaaatg atcttgctca agatttgtca acgaatcttt gaattcgttt tgaagttctg
      781 aggaaggtat catttaaaaa ctcaatctga ctttcatcat ttcgtttact cccattttga
      841 tcaccttcta ccaacaattt attaaagtga ttatactagg tttttatttt tttaattcac
      901 aattgtttat tcacttca
//
LOCUS       DARBET010000210          905 bp    DNA     linear   BCT 07-NOV-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4027
            SAMEA114569841-rid20112183.denovo.217, whole genome shotgun
            sequence.
ACCESSION   DARBET010000210 DARBET010000000
VERSION     DARBET010000210.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA114569841
            Sequence Read Archive: ERR12203666
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 905)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 905)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001972618.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001972618.1
            Genome Coverage       :: 132x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/07/2023 13:18:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,041
            CDSs (total)                      :: 2,975
            Genes (coding)                    :: 2,908
            CDSs (with protein)               :: 2,908
            Genes (RNA)                       :: 66
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 67
            CDSs (without protein)            :: 67
            Pseudo Genes (ambiguous residues) :: 0 of 67
            Pseudo Genes (frameshifted)       :: 26 of 67
            Pseudo Genes (incomplete)         :: 28 of 67
            Pseudo Genes (internal stop)      :: 20 of 67
            Pseudo Genes (multiple problems)  :: 6 of 67
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..905
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA114569841-rid20112183.denovo.217"
                     /isolate="VRE4027"
                     /db_xref="taxon:1352"
                     /country="Denmark"
     gene            265..888
                     /locus_tag="SCJ86_002945"
     CDS             265..888
                     /locus_tag="SCJ86_002945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288897.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HEG1170416.1"
                     /translation="MYLAYLRVSSDDQSLARQHKLIENWSVKNNIAEDDLKIFEEKVS
                     GKNIENRPELQELISFIRERDTIIIPSLDRLARNSKDTKDLLQLFRSKGASIEILDLP
                     SFNGVTDPALRDLLTNLVIEVFSYVAENERKKIKERQKQGIEIAKKNGRYTGKKIQYH
                     ANATGKNKLIYDEIVDGLKKKETISKIAKNAGVTRKTVYRVKRDLGM"
BASE COUNT          334 a          129 c          167 g          275 t
ORIGIN      
        1 tagttttaaa ataaactaat gtccttattt ttgtgttcac atttctgtta cctaagaaat
       61 acttaaagga ggtgatgtag ccagcaacga ctacaacttg aacccgctat ctacagttta
      121 tttgttgttt cttttgggta tactctaatg gtacaaatcc atatttgctt aaagcgttga
      181 tttttcagcg gtttttcttt attgcaaagt gtaccctaag acctgtatac taatagtaaa
      241 tagattggat aggaatgatg gcaaatgtat ttagcttatt taagagtttc ttccgacgat
      301 caaagtttag ctcggcaaca taagctaatt gaaaattggt cagtcaaaaa taatattgct
      361 gaagacgatt taaaaatttt cgaggaaaaa gtctctggaa aaaatattga aaacagacca
      421 gagctgcaag agcttatttc tttcatacgt gaacgagata cgattattat tcctagtctt
      481 gatcgattag caagaaatag taaagataca aaagacttac ttcaattgtt tcgtagtaaa
      541 ggtgcttcta ttgaaatatt agacctaccg tcgtttaatg gggtaactga ccctgcatta
      601 agagacttgc taacaaattt agtcatagaa gtgtttagct atgttgctga aaacgaacgg
      661 aaaaaaatca aagagcgtca aaaacaaggc attgaaattg caaagaaaaa tggtcgttat
      721 actggtaaaa agattcaata ccatgccaat gcaacaggaa aaaacaaatt gatttacgat
      781 gaaattgttg atggtctgaa gaaaaaagaa actatttcta aaatagcaaa gaacgcagga
      841 gttacaagaa aaaccgttta tcgagtaaaa agagatttag gaatgtaata tgttccagtt
      901 agaaa
//
LOCUS       DARBET010000211          851 bp    DNA     linear   BCT 07-NOV-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4027
            SAMEA114569841-rid20112183.denovo.219, whole genome shotgun
            sequence.
ACCESSION   DARBET010000211 DARBET010000000
VERSION     DARBET010000211.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA114569841
            Sequence Read Archive: ERR12203666
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 851)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 851)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001972618.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001972618.1
            Genome Coverage       :: 132x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/07/2023 13:18:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,041
            CDSs (total)                      :: 2,975
            Genes (coding)                    :: 2,908
            CDSs (with protein)               :: 2,908
            Genes (RNA)                       :: 66
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 67
            CDSs (without protein)            :: 67
            Pseudo Genes (ambiguous residues) :: 0 of 67
            Pseudo Genes (frameshifted)       :: 26 of 67
            Pseudo Genes (incomplete)         :: 28 of 67
            Pseudo Genes (internal stop)      :: 20 of 67
            Pseudo Genes (multiple problems)  :: 6 of 67
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..851
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA114569841-rid20112183.denovo.219"
                     /isolate="VRE4027"
                     /db_xref="taxon:1352"
                     /country="Denmark"
     gene            136..309
                     /locus_tag="SCJ86_002946"
     CDS             136..309
                     /locus_tag="SCJ86_002946"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291299.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEG1170417.1"
                     /translation="MMKRLYYSLIITIGYLIVSNLGNMVFGISKEFSWTTTLWESLFF
                     FIFVFLLQNYRKK"
BASE COUNT          283 a          142 c          104 g          322 t
ORIGIN      
        1 ttttcggaga attaaaaaat acaataaata actttagtta ctttaatgcg ttcttacttt
       61 atttttatat tatcattttt cttattcact tctttggttt atactattct tataaataaa
      121 aacgaggtat gtcctatgat gaaaagattg tactattcat tgatcataac gattggctat
      181 ttgattgtat caaatctagg aaatatggta tttggtatca gtaaagaatt cagttggact
      241 actacattat gggaatctct attctttttc atcttcgtat ttttgttaca aaattatcga
      301 aaaaaataaa catgtgccat ctatctgttg gcacatgttt taggatttat ggtacttttt
      361 attcggcaat ccattttatt tttctacttc ttcttaatac ctctaacact tttgatttat
      421 aaactaatcc aatgcatcta cttgcgctta ttaagctatt tcagctagat agtaccaccc
      481 catgcgtcaa tgcttgattt tccttttgta taacattgta taagcaataa aggacaaata
      541 agtggtgatt acaccaaacc attgaataag aaaaattctg atacgtaaat aaacatgaac
      601 ttgaaaaaat taatgaatcg gttagctact acgtagttgt cttttttctc atacagttct
      661 gttaacttac acttctcttc gctagataat aaaaaatcct ccaactgtat tgtccaatat
      721 aatcccaggc atctaaacta gcattataga agagacacac atagaatcta aaataactgg
      781 ttttaaatgt ttcttacaat caaaaaaagc tgttaaatgc actccacacc ttcatactct
      841 atcatttttt a
//
LOCUS       DARBET010000212          805 bp    DNA     linear   BCT 07-NOV-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4027
            SAMEA114569841-rid20112183.denovo.220, whole genome shotgun
            sequence.
ACCESSION   DARBET010000212 DARBET010000000
VERSION     DARBET010000212.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA114569841
            Sequence Read Archive: ERR12203666
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 805)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 805)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001972618.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001972618.1
            Genome Coverage       :: 132x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/07/2023 13:18:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,041
            CDSs (total)                      :: 2,975
            Genes (coding)                    :: 2,908
            CDSs (with protein)               :: 2,908
            Genes (RNA)                       :: 66
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 67
            CDSs (without protein)            :: 67
            Pseudo Genes (ambiguous residues) :: 0 of 67
            Pseudo Genes (frameshifted)       :: 26 of 67
            Pseudo Genes (incomplete)         :: 28 of 67
            Pseudo Genes (internal stop)      :: 20 of 67
            Pseudo Genes (multiple problems)  :: 6 of 67
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..805
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA114569841-rid20112183.denovo.220"
                     /isolate="VRE4027"
                     /db_xref="taxon:1352"
                     /country="Denmark"
     gene            6..>805
                     /locus_tag="SCJ86_002947"
     CDS             6..>805
                     /locus_tag="SCJ86_002947"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS256-like element ISEfa13 family transposase"
                     /protein_id="HEG1170418.1"
                     /translation="MLINKGDLDDLFRRHLELAINALLQAELTVFLDYEKYDRAGFNS
                     GNSRNGNYSRSFKTEYGELNLVIPRDRNGDFSQQTLPAYKRTNDSLETTIIQLVQKGI
                     TMSEISDLIEKMYGHYYKPQTISNMSKIVSEDVSAFKERTLEAKYSVIFMDATHIPLK
                     RQTVSKEAVYIVIGIRLDGTKEVLGFTIAPTESAYVWKEILQDLKDRGLEEVLLVVTD
                     GLSGIDDSIHSVYPNAQFQQCCVHISRNIAHKVRVSDRQEICSDFKLV"
BASE COUNT          274 a          117 c          149 g          265 t
ORIGIN      
        1 tggaaatact aatcaataaa ggtgatttgg atgatttgtt tcgtcgtcac ctagaattgg
       61 ctattaacgc tttattacaa gctgaattaa cggtatttct tgactatgaa aagtatgata
      121 gagctggatt taattcaggt aattcccgta atgggaatta ttcgcgctct tttaaaacag
      181 agtacggtga attgaattta gtaataccta gagataggaa tggagacttt tctcaacaaa
      241 ctttacctgc ttataaaaga accaatgact ctttagaaac cactattatt cagctagttc
      301 aaaaaggaat tactatgtcc gaaatctctg atctaatcga aaaaatgtat ggtcattatt
      361 acaaaccaca aactatttca aatatgagta aaattgtgtc tgaagatgtt tcggcattta
      421 aagaaagaac tttagaagct aaatactcag tcatttttat ggatgctact catattccct
      481 taaaaagaca aaccgtatca aaagaagctg tctatatagt aattggtatc aggttggatg
      541 gaactaaaga ggttctagga tttactatag ctccaactga atctgcttat gtttggaaag
      601 agattcttca agatctaaaa gaccgtggtt tagaagaggt tttgttagtc gtaacagatg
      661 gtttaagtgg tattgacgat agtattcata gtgtttatcc aaatgctcaa tttcaacaat
      721 gttgtgtcca tatatctaga aatatcgctc ataaggttcg tgttagtgat cgacaagaaa
      781 tctgtagcga tttcaaatta gttta
//
LOCUS       DARBET010000213          756 bp    DNA     linear   BCT 07-NOV-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4027
            SAMEA114569841-rid20112183.denovo.221, whole genome shotgun
            sequence.
ACCESSION   DARBET010000213 DARBET010000000
VERSION     DARBET010000213.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA114569841
            Sequence Read Archive: ERR12203666
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 756)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 756)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001972618.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001972618.1
            Genome Coverage       :: 132x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/07/2023 13:18:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,041
            CDSs (total)                      :: 2,975
            Genes (coding)                    :: 2,908
            CDSs (with protein)               :: 2,908
            Genes (RNA)                       :: 66
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 67
            CDSs (without protein)            :: 67
            Pseudo Genes (ambiguous residues) :: 0 of 67
            Pseudo Genes (frameshifted)       :: 26 of 67
            Pseudo Genes (incomplete)         :: 28 of 67
            Pseudo Genes (internal stop)      :: 20 of 67
            Pseudo Genes (multiple problems)  :: 6 of 67
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..756
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA114569841-rid20112183.denovo.221"
                     /isolate="VRE4027"
                     /db_xref="taxon:1352"
                     /country="Denmark"
     gene            complement(91..>756)
                     /locus_tag="SCJ86_002948"
     CDS             complement(91..>756)
                     /locus_tag="SCJ86_002948"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611768.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS3-like element ISEfa8 family transposase"
                     /protein_id="HEG1170419.1"
                     /translation="RRRIGRIMKRRGLTSTYTIAHFKGQRTACNEAKTANVLDRTFTQ
                     EQPLEAIVTDLTYVRVGKKWHYICLILDLFNREIIGYSCGEKKDASLVKEAFGRIPYS
                     LTDVKLFHTDRGKEFDNQTIHEILNGFGITRSLSRKGCPYDNAVVESTYKSVKVEFVH
                     QYQFETLAQLRLELFDYVHWWNYLRLHGTLAYETPIQIRQQRLAKRILDNERGSDTSG
                     EAA"
BASE COUNT          204 a          178 c          146 g          228 t
ORIGIN      
        1 ttgtgaatgc tgcggtgtta tggctacgat tgcccttgac aatgagcatc ctcggatctg
       61 attttctccg gcagaagcac aatcattcaa ttacgctgcc tctccagagg tatcagatcc
      121 gcgctcatta tcaaggattc gcttcgccaa tctctgttgt cgaatttgga tcggtgtttc
      181 atacgccaac gtgccatgta agcgtagata gttccaccaa tgcacataat caaacaattc
      241 tagacgtagc tgtgccagtg tctcaaattg gtattgatgc acgaattcta ctttgacaga
      301 tttataggtt gattccacaa cggcattatc atacggacaa cccttcctac tcaatgaacg
      361 agtaattcca aaaccattca gaatctcatg aatggtttgg ttatcaaatt cctttccccg
      421 gtctgtatga aaaagcttga cgtctgttaa agaatacggt atccgtccaa aggcttcttt
      481 taccaatgag gcatctttct tctcaccaca ggaataacca ataatttctc gattaaacaa
      541 atcaagtatt aagcagatat aatgccactt tttccccacg cgaacataag taagatccgt
      601 aacgatggct tccaatggct gttcttgtgt aaaggtccga tctaatacat tcgctgtttt
      661 cgcttcatta caagctgttc gttgcccttt aaaatgagcg atcgtatagg tagatgtcaa
      721 tccgcggcgt ttcatgattc gaccgattcg gcgccg
//
LOCUS       DARBET010000214          754 bp    DNA     linear   BCT 07-NOV-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4027
            SAMEA114569841-rid20112183.denovo.222, whole genome shotgun
            sequence.
ACCESSION   DARBET010000214 DARBET010000000
VERSION     DARBET010000214.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA114569841
            Sequence Read Archive: ERR12203666
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 754)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 754)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001972618.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001972618.1
            Genome Coverage       :: 132x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/07/2023 13:18:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,041
            CDSs (total)                      :: 2,975
            Genes (coding)                    :: 2,908
            CDSs (with protein)               :: 2,908
            Genes (RNA)                       :: 66
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 67
            CDSs (without protein)            :: 67
            Pseudo Genes (ambiguous residues) :: 0 of 67
            Pseudo Genes (frameshifted)       :: 26 of 67
            Pseudo Genes (incomplete)         :: 28 of 67
            Pseudo Genes (internal stop)      :: 20 of 67
            Pseudo Genes (multiple problems)  :: 6 of 67
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..754
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA114569841-rid20112183.denovo.222"
                     /isolate="VRE4027"
                     /db_xref="taxon:1352"
                     /country="Denmark"
     gene            complement(78..437)
                     /locus_tag="SCJ86_002949"
                     /pseudo
     CDS             complement(78..437)
                     /locus_tag="SCJ86_002949"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611166.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
BASE COUNT          216 a          138 c          153 g          247 t
ORIGIN      
        1 ttgtagtgac ccccaaaagt tagacttttt tggagtggag aaatccactc tttttttcat
       61 gctctaaaat gatattttta cacaaccgta acggttgttt tacgaaattg gacgggactt
      121 tgccagttta atttctgttt tatccgttca ttattgtagt aatagatata gctatcgatg
      181 gctgatttta attcctcgaa acagttataa actttcccgt gaaaaatctc ttgttttaat
      241 aagccaaaaa aattctccat aagtgaatta tctaagcagt tccctttccg agacatgctt
      301 tgaaagattt tgtactcctt caatttgttt ctataagctt tcatttgata cgaccaacct
      361 tgatctgagt gaaacgtacg acgataggga caatcgttag tggtctggat agcttcctcc
      421 aaaccttcca ttaccgcacg tgtttctgaa tggtggtagc gaccgatttg aacggctagg
      481 gcggaaatac ggtgcaaatg cacggtggtt tcatgcaatg cttacagggc gtattttgta
      541 agggtatgta acggagataa tcccgacggt aatggatgtg agaggccaag ccgacgctga
      601 cggtagggca aacaaacata gttgcactct agcgatcgtt atttattttt aacggtcaaa
      661 ctagggtaca actatgctta aaacacttcg cctcaacttc tggtagcaaa agtgattttg
      721 aaagtcaaaa tattttttat gagttattta taat
//
LOCUS       DARBET010000215          749 bp    DNA     linear   BCT 07-NOV-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4027
            SAMEA114569841-rid20112183.denovo.223, whole genome shotgun
            sequence.
ACCESSION   DARBET010000215 DARBET010000000
VERSION     DARBET010000215.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA114569841
            Sequence Read Archive: ERR12203666
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 749)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 749)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001972618.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001972618.1
            Genome Coverage       :: 132x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/07/2023 13:18:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,041
            CDSs (total)                      :: 2,975
            Genes (coding)                    :: 2,908
            CDSs (with protein)               :: 2,908
            Genes (RNA)                       :: 66
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 67
            CDSs (without protein)            :: 67
            Pseudo Genes (ambiguous residues) :: 0 of 67
            Pseudo Genes (frameshifted)       :: 26 of 67
            Pseudo Genes (incomplete)         :: 28 of 67
            Pseudo Genes (internal stop)      :: 20 of 67
            Pseudo Genes (multiple problems)  :: 6 of 67
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..749
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA114569841-rid20112183.denovo.223"
                     /isolate="VRE4027"
                     /db_xref="taxon:1352"
                     /country="Denmark"
     gene            325..723
                     /gene="spxA"
                     /locus_tag="SCJ86_002950"
     CDS             325..723
                     /gene="spxA"
                     /locus_tag="SCJ86_002950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003127030.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator Spx"
                     /protein_id="HEG1170420.1"
                     /translation="MLTLYTSPSCTSCRKARAWLQEHQIPFVERNIFSEPLNSSELKA
                     ILQMTEDGTEEIISTRSKVFQKLNMDLDDLPLQELLELVQNNPGLLRRPIMIDDKRLQ
                     VGFNEDEIRRFLPRDVRQLELRQAQLMAGL"
BASE COUNT          276 a          104 c          135 g          234 t
ORIGIN      
        1 ttaaagaaat aaaaaacata aaaaatctta agaaatcggt ttcatttagc ttttgagata
       61 aaataatagt acagttttat acgctgtttc atatgtttag aaaatttgaa ttagatttga
      121 aaactctaat ttttaaatga taaaaaaagc gagaatgctg gttttactgt atattaacct
      181 tagataactt tcattttttt aaaaaaatta tgaaaaaaag gtagctattt aaaagttgat
      241 tatgtataat agaaacagat gggagattat tatgatcttc tgttacaaaa aagtgattct
      301 tatcacgaaa aggagtggtg cacgttgtta acattatata cttccccaag ttgtacttcc
      361 tgcagaaagg ctcgtgcgtg gttgcaagaa catcagattc catttgttga acgtaatatt
      421 ttttcagaac cattgaacag ttctgaatta aaagcgattt tacaaatgac agaagacgga
      481 acagaagaaa tcatctcgac acgttccaaa gtattccaaa aactgaacat ggatttagat
      541 gatctgccgt tacaagaatt attggaactt gtgcaaaaca atcctggatt gcttcgccgt
      601 ccaatcatga ttgatgataa acgtctgcaa gttggtttta atgaagatga aattcgtcgg
      661 ttcctaccgc gcgatgtacg acaattagaa ttacgtcaag cacaattgat ggctggatta
      721 tagaaggata atacgaaact tggaaaaga
//
LOCUS       DARBET010000216          748 bp    DNA     linear   BCT 07-NOV-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4027
            SAMEA114569841-rid20112183.denovo.224, whole genome shotgun
            sequence.
ACCESSION   DARBET010000216 DARBET010000000
VERSION     DARBET010000216.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA114569841
            Sequence Read Archive: ERR12203666
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 748)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 748)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001972618.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001972618.1
            Genome Coverage       :: 132x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/07/2023 13:18:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,041
            CDSs (total)                      :: 2,975
            Genes (coding)                    :: 2,908
            CDSs (with protein)               :: 2,908
            Genes (RNA)                       :: 66
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 67
            CDSs (without protein)            :: 67
            Pseudo Genes (ambiguous residues) :: 0 of 67
            Pseudo Genes (frameshifted)       :: 26 of 67
            Pseudo Genes (incomplete)         :: 28 of 67
            Pseudo Genes (internal stop)      :: 20 of 67
            Pseudo Genes (multiple problems)  :: 6 of 67
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..748
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA114569841-rid20112183.denovo.224"
                     /isolate="VRE4027"
                     /db_xref="taxon:1352"
                     /country="Denmark"
     gene            330..>748
                     /locus_tag="SCJ86_002951"
     CDS             330..>748
                     /locus_tag="SCJ86_002951"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD-IIB family hydrolase"
                     /protein_id="HEG1170421.1"
                     /translation="MDEMKLIGIDLDGTLLNSDQSISQKNARTMKHLPEDCFPFICSG
                     REVEDINNILKKSGLRIPAVGLNGSVGFDGEKKIFESSFDKNDVKKIDSVLSKFPTKI
                     YTNLGSYESQNYKNKMKKIFHEVGSEFSIKELNYELE"
BASE COUNT          283 a           83 c          124 g          258 t
ORIGIN      
        1 aaggcaaaaa gatgaactag aaatagttca tctttttttg tttaaatctt ttgctaagtt
       61 gaactaatag agtcttttat gaatatcgaa gttagttttt cacctaactt cgttttttat
      121 agttggttat tataaaatag taatatagct gatttggatt caattcatat ttatttataa
      181 atcaaaaata cagagaatag tatataaatg tcctttccta ttcttaaaat gatgtttgta
      241 cttacttaaa ttaggtataa ttagtatatg ttttgtgaga aatttagact tacaataagc
      301 agtgaattta taatcaacgt ataggagaca tggatgaaat gaaactaatt ggtatagatt
      361 tagacggtac tcttttgaat tctgaccaga gcattagtca aaaaaatgca agaacaatga
      421 aacatttacc ggaagactgt tttccattca tttgttctgg aagagaagta gaagacatta
      481 ataatattct caaaaaatct ggtttaagga taccagcagt agggttaaat ggttctgttg
      541 gatttgatgg agaaaaaaag atatttgagt cttccttcga taaaaatgat gtaaaaaaaa
      601 ttgattctgt cctatcgaaa tttccaacca aaatatatac aaatctcgga agttatgaat
      661 ctcagaatta taaaaataaa atgaaaaaaa tttttcacga ggtagggagt gaattttcta
      721 tcaaagagct aaactatgag cttgagta
//
LOCUS       DARBET010000217          733 bp    DNA     linear   BCT 07-NOV-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4027
            SAMEA114569841-rid20112183.denovo.225, whole genome shotgun
            sequence.
ACCESSION   DARBET010000217 DARBET010000000
VERSION     DARBET010000217.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA114569841
            Sequence Read Archive: ERR12203666
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 733)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 733)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001972618.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001972618.1
            Genome Coverage       :: 132x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/07/2023 13:18:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,041
            CDSs (total)                      :: 2,975
            Genes (coding)                    :: 2,908
            CDSs (with protein)               :: 2,908
            Genes (RNA)                       :: 66
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 67
            CDSs (without protein)            :: 67
            Pseudo Genes (ambiguous residues) :: 0 of 67
            Pseudo Genes (frameshifted)       :: 26 of 67
            Pseudo Genes (incomplete)         :: 28 of 67
            Pseudo Genes (internal stop)      :: 20 of 67
            Pseudo Genes (multiple problems)  :: 6 of 67
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..733
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA114569841-rid20112183.denovo.225"
                     /isolate="VRE4027"
                     /db_xref="taxon:1352"
                     /country="Denmark"
     gene            <1..>733
                     /locus_tag="SCJ86_002952"
     rRNA            <1..>733
                     /locus_tag="SCJ86_002952"
                     /product="23S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF02541"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF02541"
BASE COUNT          226 a          146 c          216 g          145 t
ORIGIN      
        1 attgtgtgaa tacatagcac attcgaggta gacgcagaga actgaaacat ctaagtacct
       61 gcaggaagag aaagaaaatt cgattccctg agtagcggcg agcgaaacgg gaaaagccca
      121 aaccagcaag cttgcttgtt ggggttgtag gactccaata tggtagttct ttcagatagt
      181 cgaatgactt ggaaaagtca gtcaaagagg gtaaaaaccc cgtagacgaa atgtggaaga
      241 cacctaggag gatcctgagt acggcggaac acgagaaatt ccgtcggaat ccgggaggac
      301 catctcccaa ggctaaatac tccctagtga ccgatagtga accagtaccg tgagggaaag
      361 gtgaaaagca ccccggaagg ggagtgaaat agaacctgaa accgtgtgcc tacaacaagt
      421 caaagcccgt taatgggtga tggcgtgcct tttgtagaat gaaccggcga gttacgattg
      481 catgcgaggt taagttgaag agacggagcc gcagcgaaag cgagtctgaa tagggcgttt
      541 gagtatgtag tcgtagaccc gaaaccatgt gatctaccca tgtccaggtt gaaggtgcgg
      601 taaaacgcac tggaggaccg aacccacgta cgttgaaaag tgcggggatg aggtgtgggt
      661 agcggagaaa ttccaaacga acttggagat agctggttct ctccgaaata gctttagggc
      721 tagcctcgga att
//
LOCUS       DARBET010000218          712 bp    DNA     linear   BCT 07-NOV-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4027
            SAMEA114569841-rid20112183.denovo.227, whole genome shotgun
            sequence.
ACCESSION   DARBET010000218 DARBET010000000
VERSION     DARBET010000218.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA114569841
            Sequence Read Archive: ERR12203666
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 712)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 712)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001972618.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001972618.1
            Genome Coverage       :: 132x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/07/2023 13:18:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,041
            CDSs (total)                      :: 2,975
            Genes (coding)                    :: 2,908
            CDSs (with protein)               :: 2,908
            Genes (RNA)                       :: 66
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 67
            CDSs (without protein)            :: 67
            Pseudo Genes (ambiguous residues) :: 0 of 67
            Pseudo Genes (frameshifted)       :: 26 of 67
            Pseudo Genes (incomplete)         :: 28 of 67
            Pseudo Genes (internal stop)      :: 20 of 67
            Pseudo Genes (multiple problems)  :: 6 of 67
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..712
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA114569841-rid20112183.denovo.227"
                     /isolate="VRE4027"
                     /db_xref="taxon:1352"
                     /country="Denmark"
     gene            complement(<1..446)
                     /locus_tag="SCJ86_002953"
     CDS             complement(<1..446)
                     /locus_tag="SCJ86_002953"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002316575.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HEG1170422.1"
                     /translation="MNYEFFLSKEDHRSYSLLKYLEASSNLSESISNVQEELSLSTFL
                     LKKTIDKLKHDLQKFDLEKNLRVTVSELDICLEINGKCSSKALLSRYITDSLSMKMVL
                     AFFQEKYISVENFAKQHHVSYSVAYKVLQRLKRNLKKYQIFFEKRK"
BASE COUNT          216 a          123 c           95 g          278 t
ORIGIN      
        1 aactttctct tttcaaaaaa aatttgatat ttttttagat ttctttttag tctttgtaat
       61 actttataag ccacagaata actcacgtga tgctgtttag caaaattttc tactgagata
      121 tatttttcct gaaagaaagc caataccatt ttcatagata aagaatccgt tatataacga
      181 gataacagag ctttgctaga acattttcca ttaatttcca gacatatatc caattcactg
      241 acggttactc gcaaattttt ctctagatcg aacttttgta aatcatgttt cagcttatcg
      301 atcgtttttt tcaagagaaa tgtcgatagc gacaactctt cttgaacatt cgatattgac
      361 tctgataaat ttgagcttgc ttctaaatat ttaagtaatg aataacttct gtgatcttct
      421 ttgcttaaaa aaaactcata attcattagt catcagctct ttctacgtat attactttcc
      481 atgcattcac caactagctg gggataaaac tgatttcctg gatccgagtc actgttgtca
      541 gcgattcttg tttgtataat atacatcgtc ctgtcttttt tttaacattt tggagagcgt
      601 tttatttggc ctttctcgcg atcaaacctg ttattagaat tatatcattt actagtatgc
      661 atttttatta ataaaatggt atattttata ttgttataaa aagagcccaa cc
//
LOCUS       DARBET010000219          704 bp    DNA     linear   BCT 07-NOV-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4027
            SAMEA114569841-rid20112183.denovo.228, whole genome shotgun
            sequence.
ACCESSION   DARBET010000219 DARBET010000000
VERSION     DARBET010000219.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA114569841
            Sequence Read Archive: ERR12203666
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 704)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 704)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001972618.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001972618.1
            Genome Coverage       :: 132x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/07/2023 13:18:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,041
            CDSs (total)                      :: 2,975
            Genes (coding)                    :: 2,908
            CDSs (with protein)               :: 2,908
            Genes (RNA)                       :: 66
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 67
            CDSs (without protein)            :: 67
            Pseudo Genes (ambiguous residues) :: 0 of 67
            Pseudo Genes (frameshifted)       :: 26 of 67
            Pseudo Genes (incomplete)         :: 28 of 67
            Pseudo Genes (internal stop)      :: 20 of 67
            Pseudo Genes (multiple problems)  :: 6 of 67
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..704
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA114569841-rid20112183.denovo.228"
                     /isolate="VRE4027"
                     /db_xref="taxon:1352"
                     /country="Denmark"
BASE COUNT          298 a          106 c          122 g          178 t
ORIGIN      
        1 aattccttag gcagcttttg ctgtgtagaa aggaataaat aataacgaaa gttcaaaagc
       61 taatgccgac tattctagac attaaagaag acgaaaacga acgataaaga aagaaaagtt
      121 atgaataatc gtttattgaa aaaaacttga aaaaatacaa aaaattcgtt gaccaagcta
      181 aatttagttg gtatattatt acttgtccaa aagacagaag aaaatattta ttcaaaaaca
      241 taacaaaaaa gttattgaca tgttactaac gacatgataa gataactgag tcgttaaaaa
      301 agaaccactt gcgaagcttg attctatcga aaaaaacttc aaaaaagttc ttgacaaata
      361 acaaacgata agttatgata aatgagttgc cgaaagattt cgaaaaacga aatcgaaaaa
      421 aacttttaaa aaagagttga caatcgcttg tctctctgat atgatataaa agttgctacg
      481 gcagccaaaa atgaatcggg aaacactctc gatgaagtag acctttgaaa actgaacaaa
      541 gtaagacaaa ccaaatgtgt agggcgtctt gattcaattc aagacaacaa acatttttaa
      601 caagcaagca atatgctagc aaacaaattg agcttaacaa tcgcaagatt gttcgaactt
      661 tttatgagag tttgatcctg gctcaggacg aacgctggcg gcgt
//
LOCUS       DARBET010000220          661 bp    DNA     linear   BCT 07-NOV-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4027
            SAMEA114569841-rid20112183.denovo.230, whole genome shotgun
            sequence.
ACCESSION   DARBET010000220 DARBET010000000
VERSION     DARBET010000220.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA114569841
            Sequence Read Archive: ERR12203666
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 661)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 661)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001972618.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001972618.1
            Genome Coverage       :: 132x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/07/2023 13:18:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,041
            CDSs (total)                      :: 2,975
            Genes (coding)                    :: 2,908
            CDSs (with protein)               :: 2,908
            Genes (RNA)                       :: 66
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 67
            CDSs (without protein)            :: 67
            Pseudo Genes (ambiguous residues) :: 0 of 67
            Pseudo Genes (frameshifted)       :: 26 of 67
            Pseudo Genes (incomplete)         :: 28 of 67
            Pseudo Genes (internal stop)      :: 20 of 67
            Pseudo Genes (multiple problems)  :: 6 of 67
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..661
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA114569841-rid20112183.denovo.230"
                     /isolate="VRE4027"
                     /db_xref="taxon:1352"
                     /country="Denmark"
     gene            complement(31..>661)
                     /locus_tag="SCJ86_002954"
     CDS             complement(31..>661)
                     /locus_tag="SCJ86_002954"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_021424053.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="aminoglycoside phosphotransferase family
                     protein"
                     /protein_id="HEG1170423.1"
                     /translation="ILGYKEIKGTFLTPEIYSTMSEEEQNLLKRDIASFLRQMHGLDY
                     TDISECTIDNKQNVLEEYILLRETIYNDLTDIEKDYIESFMERLNATTVFEGKKCLCH
                     NDFSCNHLLLDGNNRLTGIIDFGDSGIIDEYCDFIYLLEDSEEEIGTNFGEDILRMYG
                     NIDIEKAKEYQDIVEEYYPIETIVYGIKNIKQEFIENGRKEIYKRTYKD"
BASE COUNT          221 a          113 c           44 g          283 t
ORIGIN      
        1 ttttataata gcttttcata tattatataa tcaatcttta taagtccttt tataaatttc
       61 ttttctacca ttttcgataa attcctgttt aatattttta attccataaa caatagtttc
      121 aataggataa tattcttcaa ctatatcttg atattctttt gctttctcaa tatctatatt
      181 tccatacatt cttaatatat cttctccaaa atttgttcct atttcttctt cactatcttc
      241 aagtaagtat ataaaatcac aatattcatc tataattcca gaatctccaa aatcaattat
      301 tccagttaat ctattattgc catctaacaa tagatgatta caactaaaat cattatggca
      361 taaacacttt ttaccctcaa aaactgttgt tgcatttagt ctttccataa aactttctat
      421 ataatctttt tctatatcag ttaaatcatt ataaatagtt tcacgcaaca atatatactc
      481 ttctaataca ttttgtttat tatcaatagt acattcacta atatctgtat aatctaaacc
      541 gtgcatttgt cttaaaaaac tggcaatatc tcgttttaac aaattttgtt cttcttctga
      601 catagtagaa taaatttctg gtgttaaaaa agttccttta atttctttat aacctagtat
      661 a
//
LOCUS       DARBET010000221          635 bp    DNA     linear   BCT 07-NOV-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4027
            SAMEA114569841-rid20112183.denovo.232, whole genome shotgun
            sequence.
ACCESSION   DARBET010000221 DARBET010000000
VERSION     DARBET010000221.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA114569841
            Sequence Read Archive: ERR12203666
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 635)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 635)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001972618.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001972618.1
            Genome Coverage       :: 132x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/07/2023 13:18:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,041
            CDSs (total)                      :: 2,975
            Genes (coding)                    :: 2,908
            CDSs (with protein)               :: 2,908
            Genes (RNA)                       :: 66
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 67
            CDSs (without protein)            :: 67
            Pseudo Genes (ambiguous residues) :: 0 of 67
            Pseudo Genes (frameshifted)       :: 26 of 67
            Pseudo Genes (incomplete)         :: 28 of 67
            Pseudo Genes (internal stop)      :: 20 of 67
            Pseudo Genes (multiple problems)  :: 6 of 67
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..635
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA114569841-rid20112183.denovo.232"
                     /isolate="VRE4027"
                     /db_xref="taxon:1352"
                     /country="Denmark"
     gene            complement(<1..270)
                     /locus_tag="SCJ86_002955"
     CDS             complement(<1..270)
                     /locus_tag="SCJ86_002955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347917.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEG1170424.1"
                     /translation="MFKPLIDSYSAVLKKFKGKDISATINEEVNIDRLKTMYDGYDGD
                     RVIEIRFIDPRRFTVQQRNFIYALIGDIFIDTGMPTDFWKEFFYFR"
     gene            complement(276..>635)
                     /locus_tag="SCJ86_002956"
     CDS             complement(276..>635)
                     /locus_tag="SCJ86_002956"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340820.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1071 domain-containing protein"
                     /protein_id="HEG1170425.1"
                     /translation="RNQPVVKPNAMQINNSLKRCFVKALALHGLGLYVFQGEDIPTPP
                     RIDTKKLNMLETILEAFNEQMGKDMTKTLIEYVNEQTDKLGLLADNVETIEQLSYEQC
                     ALMERAIAAKRKELDKK"
BASE COUNT          164 a          135 c          110 g          226 t
ORIGIN      
        1 acggaagtag aagaattcct tccagaagtc cgttggcatg cctgtatcga taaaaatatc
       61 gcctatcagc gcatagatga agtttcgttg ctgtacggtg aaacgtctag gatcaataaa
      121 acgaatttca atgactcgat caccatcgta gccgtcatac atcgtcttta gtcgatcaat
      181 gttcacttcc tcattgatgg ttgcgcttat gtcttttcct ttgaactttt tcagaaccgc
      241 tgaatatgaa tcgattaatg gtttaaacac tcatatcact tcttatctaa ttcttttctc
      301 ttagctgcta ttgctcgctc catcaaggca cattgctcat agcttaactg ttcaatagtt
      361 tcaacgttat cagctaagag ccctaattta tctgtctgct cattaacata ttcgattaag
      421 gttttggtca tatctttacc catctgctca ttgaaagctt ctagaatcgt ctctagcatg
      481 tttaatttct ttgtatcgat tctaggtggt gttggaatat cttccccttg aaatacatat
      541 aatcccagtc cgtgtagagc caatgctttc acaaagcatc gcttcaatga gttattgatt
      601 tgcattgcat ttggtttaac aactggttgg tttcg
//
LOCUS       DARBET010000222          632 bp    DNA     linear   BCT 07-NOV-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4027
            SAMEA114569841-rid20112183.denovo.233, whole genome shotgun
            sequence.
ACCESSION   DARBET010000222 DARBET010000000
VERSION     DARBET010000222.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA114569841
            Sequence Read Archive: ERR12203666
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 632)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 632)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001972618.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001972618.1
            Genome Coverage       :: 132x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/07/2023 13:18:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,041
            CDSs (total)                      :: 2,975
            Genes (coding)                    :: 2,908
            CDSs (with protein)               :: 2,908
            Genes (RNA)                       :: 66
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 67
            CDSs (without protein)            :: 67
            Pseudo Genes (ambiguous residues) :: 0 of 67
            Pseudo Genes (frameshifted)       :: 26 of 67
            Pseudo Genes (incomplete)         :: 28 of 67
            Pseudo Genes (internal stop)      :: 20 of 67
            Pseudo Genes (multiple problems)  :: 6 of 67
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..632
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA114569841-rid20112183.denovo.233"
                     /isolate="VRE4027"
                     /db_xref="taxon:1352"
                     /country="Denmark"
     gene            <1..>632
                     /locus_tag="SCJ86_002957"
     CDS             <1..>632
                     /locus_tag="SCJ86_002957"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012655884.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEG1170426.1"
                     /translation="SLKKHYTEPWEDEVFRVIIEYNNVPIGYGQIYKMYDELYTDYHY
                     PKTDEIVYGMDQFIGEPNYWSKGIGTRYIKLIFEFLKKERNANAVILDPHKNNPRAIR
                     AYQKSGFRIIEDLPEHELHEGKKEDCYLMEYRYDDNATNVKAMKYLIEHYFDNFKVDS
                     IEIIGSGYDSVAYLVNNEYIFKTKFSTNKKKGYAKEKAIYNFLNTNLETN"
BASE COUNT          268 a           54 c          108 g          202 t
ORIGIN      
        1 atcattaaaa aaacattata cagagccttg ggaagatgaa gtttttagag taattattga
       61 atataacaat gttcctattg gatatggaca aatatataaa atgtatgatg agttatatac
      121 tgattatcat tatccaaaaa ctgatgagat agtctatggt atggatcaat ttataggaga
      181 gccaaattat tggagtaaag gaattggtac aagatatatt aaattgattt ttgaattttt
      241 gaaaaaagaa agaaatgcta atgcagttat tttagaccct cataaaaata atccaagagc
      301 aataagggca taccaaaaat ctggttttag aattattgaa gatttgccag aacatgaatt
      361 acacgagggc aaaaaagaag attgttattt aatggaatat agatatgatg ataatgccac
      421 aaatgttaag gcaatgaaat atttaattga gcattacttt gataatttca aagtagatag
      481 tattgaaata atcggtagtg gttatgatag tgtggcatat ttagttaata atgaatacat
      541 ttttaaaaca aaatttagta ctaataagaa aaaaggttat gcaaaagaaa aagcaatata
      601 taatttttta aatacaaatt tagaaactaa tg
//
LOCUS       DARBET010000223          567 bp    DNA     linear   BCT 07-NOV-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4027
            SAMEA114569841-rid20112183.denovo.234, whole genome shotgun
            sequence.
ACCESSION   DARBET010000223 DARBET010000000
VERSION     DARBET010000223.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA114569841
            Sequence Read Archive: ERR12203666
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 567)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 567)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001972618.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001972618.1
            Genome Coverage       :: 132x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/07/2023 13:18:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,041
            CDSs (total)                      :: 2,975
            Genes (coding)                    :: 2,908
            CDSs (with protein)               :: 2,908
            Genes (RNA)                       :: 66
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 67
            CDSs (without protein)            :: 67
            Pseudo Genes (ambiguous residues) :: 0 of 67
            Pseudo Genes (frameshifted)       :: 26 of 67
            Pseudo Genes (incomplete)         :: 28 of 67
            Pseudo Genes (internal stop)      :: 20 of 67
            Pseudo Genes (multiple problems)  :: 6 of 67
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..567
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA114569841-rid20112183.denovo.234"
                     /isolate="VRE4027"
                     /db_xref="taxon:1352"
                     /country="Denmark"
     gene            26..>567
                     /locus_tag="SCJ86_002958"
     CDS             26..>567
                     /locus_tag="SCJ86_002958"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_064305258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS6-like element IS1216 family transposase"
                     /protein_id="HEG1170427.1"
                     /translation="MNHFKGKQFQQDVIIVAVGYYLRYNLSYREVQEILYDRGINVSH
                     TTIYRWVQEYGKLLYQIWKKKNKKSFYSWKMDETYIKIKGKWHYLYRAIDADGLTLDI
                     WLRKKRDTQAAYAFLKRLVKQFDEPKVVVTDKAPSITSAFKKLKEYGFYQGTEHRTIK
                     YLNNLIEQDHRPVKRRNKFYR"
BASE COUNT          204 a           92 c          114 g          157 t
ORIGIN      
        1 gaaaaagata gcaggaggaa tgacgatgaa tcattttaaa ggaaagcaat ttcagcagga
       61 tgtgattatt gtagccgtgg gctactatct tcgttataac cttagctatc gtgaagttca
      121 agaaatctta tatgatcgtg gcattaacgt ttctcatacg acgatttatc gttgggtgca
      181 agaatatggc aaactactct atcaaatttg gaaaaagaaa aataaaaaat ccttttattc
      241 atggaaaatg gatgaaacgt acatcaaaat taaaggaaaa tggcattatt tgtatcgagc
      301 catcgatgca gatggtttaa ccttggatat ttggttacgt aaaaaacggg acacacaagc
      361 agcctatgct tttcttaagc ggttagtgaa gcagtttgat gaaccgaagg ttgtagtcac
      421 agataaagcc ccctctatta caagtgcctt taagaaacta aaagaatacg gcttttatca
      481 agggacagaa catcgtacca ttaaatacct gaataatttg attgaacaag accatcgtcc
      541 agtaaagaga cgcaataaat tctatcg
//
LOCUS       DARBET010000224          545 bp    DNA     linear   BCT 07-NOV-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4027
            SAMEA114569841-rid20112183.denovo.235, whole genome shotgun
            sequence.
ACCESSION   DARBET010000224 DARBET010000000
VERSION     DARBET010000224.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA114569841
            Sequence Read Archive: ERR12203666
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 545)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 545)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001972618.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001972618.1
            Genome Coverage       :: 132x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/07/2023 13:18:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,041
            CDSs (total)                      :: 2,975
            Genes (coding)                    :: 2,908
            CDSs (with protein)               :: 2,908
            Genes (RNA)                       :: 66
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 67
            CDSs (without protein)            :: 67
            Pseudo Genes (ambiguous residues) :: 0 of 67
            Pseudo Genes (frameshifted)       :: 26 of 67
            Pseudo Genes (incomplete)         :: 28 of 67
            Pseudo Genes (internal stop)      :: 20 of 67
            Pseudo Genes (multiple problems)  :: 6 of 67
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..545
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA114569841-rid20112183.denovo.235"
                     /isolate="VRE4027"
                     /db_xref="taxon:1352"
                     /country="Denmark"
     gene            <1..378
                     /locus_tag="SCJ86_002959"
     CDS             <1..378
                     /locus_tag="SCJ86_002959"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344895.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4062 domain-containing protein"
                     /protein_id="HEG1170428.1"
                     /translation="PIEFDNDERLLLIYAFMDENSIFSTDNSTYRKVVQWESDNNLRS
                     YLSDRVFDVLEKLSKSGILKEEYDSSFDEYPSRYILISEDYKKFKRYFLKNEKKISVI
                     MEKYKAPIQADPFSNLDESDLPF"
BASE COUNT          203 a           57 c           95 g          190 t
ORIGIN      
        1 ccaatagaat ttgacaatga tgaaagattg ctgctaatat atgcgtttat ggatgaaaat
       61 tctatctttt caactgataa ttcaacttat agaaaagtag ttcaatggga aagtgataac
      121 aatttaagga gttacttgag tgatagagta tttgatgtac tagaaaaatt aagtaaatcg
      181 ggaattttaa aggaagaata cgattcttct tttgatgagt atccatcacg ctatattttg
      241 atttcagagg actataagaa atttaaaagg tattttttaa aaaatgaaaa aaagatatct
      301 gttattatgg agaaatacaa agctccaata caggctgatc cattttccaa tttggatgaa
      361 tcagatttac cgttttaaat tttaaataga taatttcatt ttttagtagc agaggtataa
      421 acttttttaa tgtttgaatt gaaaagaaat gctaataggt cgactttatg tttctcttgt
      481 gcaatcgtaa atgcggggga ccataaaatt gatctattat cgagtgtgta taaaataaaa
      541 ttcaa
//
LOCUS       DARBET010000225          536 bp    DNA     linear   BCT 07-NOV-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4027
            SAMEA114569841-rid20112183.denovo.236, whole genome shotgun
            sequence.
ACCESSION   DARBET010000225 DARBET010000000
VERSION     DARBET010000225.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA114569841
            Sequence Read Archive: ERR12203666
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 536)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 536)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001972618.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001972618.1
            Genome Coverage       :: 132x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/07/2023 13:18:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,041
            CDSs (total)                      :: 2,975
            Genes (coding)                    :: 2,908
            CDSs (with protein)               :: 2,908
            Genes (RNA)                       :: 66
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 67
            CDSs (without protein)            :: 67
            Pseudo Genes (ambiguous residues) :: 0 of 67
            Pseudo Genes (frameshifted)       :: 26 of 67
            Pseudo Genes (incomplete)         :: 28 of 67
            Pseudo Genes (internal stop)      :: 20 of 67
            Pseudo Genes (multiple problems)  :: 6 of 67
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..536
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA114569841-rid20112183.denovo.236"
                     /isolate="VRE4027"
                     /db_xref="taxon:1352"
                     /country="Denmark"
     gene            complement(367..>536)
                     /locus_tag="SCJ86_002960"
     CDS             complement(367..>536)
                     /locus_tag="SCJ86_002960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014387120.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEG1170429.1"
                     /translation="ELTNTHIKEENVPLFVFETEQKNQREELGEGYIWEEPGQMRSVL
                     GTYEVEGVPFF"
BASE COUNT          173 a           94 c           65 g          204 t
ORIGIN      
        1 gatagaatca agcttcgcaa gtggttcttt tttaacgact cagttatctt atcatgtcgt
       61 tagtaacatg tcaataacta ttttttaaat tttttagaaa tgtgatataa catccacttc
      121 agaatcaacg ctgttaaatt tatcatgtat ttcttccttg gtcaactata aattaagcat
      181 tactttgtga aatgattttt cgatcaatta ttattattcg ttatacaacc gttttcaaca
      241 tattttgaaa ataaaatgca ctaaaaaact atctttagca gaacatttca ttgtattttt
      301 tggaccaaac aataaaaaaa gaagtttgaa aaaaattcaa acttctttgt tcgcttaatc
      361 tgtattttag aagaaaggca ctccctctac ttcgtaagtt ccaagaacag agcgcatttg
      421 acctggttct tcccaaatat atccttcgcc taattcttct ctttgattct tttgttcagt
      481 ttcaaagaca aacaacggaa cattttcctc tttaatatgt gtatttgtca attcca
//
LOCUS       DARBET010000226          526 bp    DNA     linear   BCT 07-NOV-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4027
            SAMEA114569841-rid20112183.denovo.237, whole genome shotgun
            sequence.
ACCESSION   DARBET010000226 DARBET010000000
VERSION     DARBET010000226.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA114569841
            Sequence Read Archive: ERR12203666
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 526)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 526)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001972618.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001972618.1
            Genome Coverage       :: 132x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/07/2023 13:18:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,041
            CDSs (total)                      :: 2,975
            Genes (coding)                    :: 2,908
            CDSs (with protein)               :: 2,908
            Genes (RNA)                       :: 66
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 67
            CDSs (without protein)            :: 67
            Pseudo Genes (ambiguous residues) :: 0 of 67
            Pseudo Genes (frameshifted)       :: 26 of 67
            Pseudo Genes (incomplete)         :: 28 of 67
            Pseudo Genes (internal stop)      :: 20 of 67
            Pseudo Genes (multiple problems)  :: 6 of 67
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..526
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA114569841-rid20112183.denovo.237"
                     /isolate="VRE4027"
                     /db_xref="taxon:1352"
                     /country="Denmark"
     gene            complement(6..359)
                     /gene="tnpB"
                     /locus_tag="SCJ86_002961"
     CDS             complement(6..359)
                     /gene="tnpB"
                     /locus_tag="SCJ86_002961"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001846105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS66 family insertion sequence element accessory
                     protein TnpB"
                     /protein_id="HEG1170430.1"
                     /translation="MLYDLLQTKHIYIVCGKTDLRKGIDGLASLIQQEYQLELYEDAV
                     FLFCGNRQDRFKLLYWDGDGFLLCYKRIENGKLKWPRTKDEVRTLTNQQVRWLLEGLS
                     IDQPRAILPGKKGVF"
     gene            complement(349..>526)
                     /locus_tag="SCJ86_002962"
     CDS             complement(349..>526)
                     /locus_tag="SCJ86_002962"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002285758.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEG1170431.1"
                     /translation="IVPVQLGNEKSAPEKGKPAAAKLAMRIKRENGSEVLIYNGINNY
                     ILQAILKELLSNAL"
BASE COUNT          153 a          116 c           90 g          167 t
ORIGIN      
        1 ccagcttaaa aaactccttt ttttcctggc aaaatagctc gaggttgatc gatacttaat
       61 ccttctaaca accatcgaac ctgttggttc gttaaggttc gaacttcgtc tttcgtccga
      121 ggccatttta acttgccgtt ttctatccgc ttataacaga gaagaaaacc atcaccgtcc
      181 caatagagaa gtttgaaacg gtcttgacgg ttcccgcaaa ataaaaaaac ggcatcttcg
      241 taaagctcga gttgatactc ttgttgaata agactggcaa gaccatcaat tccttttcgc
      301 aaatctgttt ttccgcacac aatataaata tgtttggttt gaagtaaatc ataaagcatt
      361 ggacaacagt tctttcaaaa tagcttgaag aatataattg ttaataccgt tatagatcaa
      421 aacttcagaa ccattctctc ttttgatgcg catcgctaac ttggcagccg cgggtttacc
      481 tttttctggt gcactctttt cgtttcctaa ttggacagga acaatc
//
LOCUS       DARBET010000227          515 bp    DNA     linear   BCT 07-NOV-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4027
            SAMEA114569841-rid20112183.denovo.238, whole genome shotgun
            sequence.
ACCESSION   DARBET010000227 DARBET010000000
VERSION     DARBET010000227.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA114569841
            Sequence Read Archive: ERR12203666
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 515)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 515)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001972618.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001972618.1
            Genome Coverage       :: 132x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/07/2023 13:18:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,041
            CDSs (total)                      :: 2,975
            Genes (coding)                    :: 2,908
            CDSs (with protein)               :: 2,908
            Genes (RNA)                       :: 66
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 67
            CDSs (without protein)            :: 67
            Pseudo Genes (ambiguous residues) :: 0 of 67
            Pseudo Genes (frameshifted)       :: 26 of 67
            Pseudo Genes (incomplete)         :: 28 of 67
            Pseudo Genes (internal stop)      :: 20 of 67
            Pseudo Genes (multiple problems)  :: 6 of 67
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..515
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA114569841-rid20112183.denovo.238"
                     /isolate="VRE4027"
                     /db_xref="taxon:1352"
                     /country="Denmark"
     gene            complement(107..>515)
                     /locus_tag="SCJ86_002963"
     CDS             complement(107..>515)
                     /locus_tag="SCJ86_002963"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342103.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="linear amide C-N hydrolase"
                     /protein_id="HEG1170432.1"
                     /translation="GTPENHFSNQISLNVYSRGMGGLGLPGDLSSVSRFVKATFTKMN
                     AASGDSESESISQFFHILGSVEQQKGVCDTGEGKYEYTIYSSCCNVDKGIYYYRTYED
                     SQITAVNMNKEDLDRHELVSYPVIKEQQINYVN"
BASE COUNT          163 a          103 c           78 g          171 t
ORIGIN      
        1 atcatccata aaatggactt tgtctacagt ctgagacacg acctaaagat cgtgtctaat
       61 tatgactgta tcagaaaata tgataccaaa cggatctatt cattaattaa ttgacatagt
      121 ttatctgttg ctcttttatg actggataac tgaccaattc atgacgatcc aagtcttctt
      181 tattcatatt tacagcagta atttgactgt cttcatacgt acgataataa taaatccctt
      241 tatcaacgtt acagcaagaa gaataaatcg tatattcgta ttttccttct ccagtatcgc
      301 atactccttt ttgttgctcg actgagccta gtatatgaaa aaattgactg atactttcag
      361 attctgaatc acctgatgca gcattcattt tagtaaatgt ggcttttaca aatcgagaaa
      421 cagaagagag atctccaggt aagcccaatc ctcccatccc acgactataa acattcaagc
      481 tgatctggtt tgaaaaatga ttttctggtg ttcca
//
LOCUS       DARBET010000228          510 bp    DNA     linear   BCT 07-NOV-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4027
            SAMEA114569841-rid20112183.denovo.239, whole genome shotgun
            sequence.
ACCESSION   DARBET010000228 DARBET010000000
VERSION     DARBET010000228.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA114569841
            Sequence Read Archive: ERR12203666
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 510)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 510)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001972618.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001972618.1
            Genome Coverage       :: 132x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/07/2023 13:18:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,041
            CDSs (total)                      :: 2,975
            Genes (coding)                    :: 2,908
            CDSs (with protein)               :: 2,908
            Genes (RNA)                       :: 66
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 67
            CDSs (without protein)            :: 67
            Pseudo Genes (ambiguous residues) :: 0 of 67
            Pseudo Genes (frameshifted)       :: 26 of 67
            Pseudo Genes (incomplete)         :: 28 of 67
            Pseudo Genes (internal stop)      :: 20 of 67
            Pseudo Genes (multiple problems)  :: 6 of 67
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..510
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA114569841-rid20112183.denovo.239"
                     /isolate="VRE4027"
                     /db_xref="taxon:1352"
                     /country="Denmark"
BASE COUNT          154 a          108 c           74 g          174 t
ORIGIN      
        1 aaccgggggc tttattcctg cagtccggct acgccttcct gcaatagaca aaactccaga
       61 tcgggtaatt cgcttgtggc gaaacccgac ctggagtttg tctctgtatt ttgaacccta
      121 aaaattaatc aaacagactg atattctcat aatcagcgcg aaataagcca ttaagtacag
      181 ttttcctacc tatttttgat aacccgctgg ttagttaata ttccttttcc gagagagata
      241 aatgattggt taaatctata tacatctcaa cctttaaaaa tgttttcgaa atacttcatc
      301 agtacatgtc atacaaatta ccagtgcatt ctcaaattct agttctttaa ttgatttcag
      361 ttgaggagca gttacttttg gcggaaattt ccatttttgt ccaaacctct tcccccgatg
      421 aaatttcttt gttgcaatta ttgcaatgaa aaatttttgt ttcaaaaata tacataatta
      481 cttcctccaa taaaaactat ctacctctca
//
LOCUS       DARBET010000229          506 bp    DNA     linear   BCT 07-NOV-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4027
            SAMEA114569841-rid20112183.denovo.240, whole genome shotgun
            sequence.
ACCESSION   DARBET010000229 DARBET010000000
VERSION     DARBET010000229.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA114569841
            Sequence Read Archive: ERR12203666
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 506)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 506)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001972618.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001972618.1
            Genome Coverage       :: 132x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/07/2023 13:18:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,041
            CDSs (total)                      :: 2,975
            Genes (coding)                    :: 2,908
            CDSs (with protein)               :: 2,908
            Genes (RNA)                       :: 66
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 67
            CDSs (without protein)            :: 67
            Pseudo Genes (ambiguous residues) :: 0 of 67
            Pseudo Genes (frameshifted)       :: 26 of 67
            Pseudo Genes (incomplete)         :: 28 of 67
            Pseudo Genes (internal stop)      :: 20 of 67
            Pseudo Genes (multiple problems)  :: 6 of 67
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..506
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA114569841-rid20112183.denovo.240"
                     /isolate="VRE4027"
                     /db_xref="taxon:1352"
                     /country="Denmark"
     gene            231..>506
                     /locus_tag="SCJ86_002964"
     CDS             231..>506
                     /locus_tag="SCJ86_002964"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000221326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HEG1170433.1"
                     /translation="MTYKHLTIDELTMIESYYLQHNKPVEIANRMGRAIQTIYNVVNK
                     FKQGKTALDYWHQYKENKKKCGRKVIQLPAHEVDYIKEKVTLGWTPDV"
BASE COUNT          190 a           83 c           88 g          145 t
ORIGIN      
        1 cgatagaatc aagcttcgca agtggttctt tttaacgact tagttatctt atcacgtcta
       61 aaaacatgtg tcaactcttt tttggaattt tcataaaatg tttcaatgaa gcgcgacaac
      121 gttgtttaat atatcgcgaa ttgtaaaatt aagttagaaa aataaaaagg catttatggt
      181 acactcaaat tgtatttccg acgaaagaaa acaaaggagt gtaaccataa atgacctaca
      241 aacatcttac catagacgaa ctgacaatga tagaatcata ttatcttcaa cataataagc
      301 cggttgaaat cgctaaccga atgggacgtg ctatacaaac tatttataat gtagtcaata
      361 agttcaagca aggcaagact gctcttgatt attggcacca gtataaagaa aataagaaaa
      421 aatgtggtag aaaagtcatt caattacctg ctcatgaagt agattacatt aaagagaaag
      481 tcactcttgg ttggacgcct gacgtc
//
LOCUS       DARBET010000230          485 bp    DNA     linear   BCT 07-NOV-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4027
            SAMEA114569841-rid20112183.denovo.241, whole genome shotgun
            sequence.
ACCESSION   DARBET010000230 DARBET010000000
VERSION     DARBET010000230.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA114569841
            Sequence Read Archive: ERR12203666
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 485)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 485)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001972618.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001972618.1
            Genome Coverage       :: 132x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/07/2023 13:18:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,041
            CDSs (total)                      :: 2,975
            Genes (coding)                    :: 2,908
            CDSs (with protein)               :: 2,908
            Genes (RNA)                       :: 66
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 67
            CDSs (without protein)            :: 67
            Pseudo Genes (ambiguous residues) :: 0 of 67
            Pseudo Genes (frameshifted)       :: 26 of 67
            Pseudo Genes (incomplete)         :: 28 of 67
            Pseudo Genes (internal stop)      :: 20 of 67
            Pseudo Genes (multiple problems)  :: 6 of 67
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..485
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA114569841-rid20112183.denovo.241"
                     /isolate="VRE4027"
                     /db_xref="taxon:1352"
                     /country="Denmark"
     gene            complement(62..259)
                     /locus_tag="SCJ86_002965"
     CDS             complement(62..259)
                     /locus_tag="SCJ86_002965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342557.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cold-shock protein"
                     /protein_id="HEG1170434.1"
                     /translation="MNNGTVKWFNSDKGFGFITGEDGNDVFAHFSAIQGEGFKSLDEG
                     QAVTYDIEEGQRGPQAVNIVK"
BASE COUNT          158 a           89 c           68 g          170 t
ORIGIN      
        1 gaattttttc taccgacata aacaaaaaaa ccacctcata tgaggcggtt aacgttcaac
       61 attattttac gatatttact gcttgaggac cacgttgtcc ttcttcaata tcataagtaa
      121 ctgcttggcc ttcatctaaa gacttaaagc cttctccttg gattgctgag aaatgagcga
      181 atacgtcatt tccatcttca ccagtgatga aaccaaaacc tttatctgag ttaaaccatt
      241 ttactgtacc gttattcata tatatttcct cctgatacgt atataatacg tgttttattg
      301 caattaatat tacttggtaa aaggagtttt tatagtgtga atatatcgct cagattacgt
      361 ttcaaattag attacttatt tactataaca cggatgtaat gctaacacaa ggataatcac
      421 tcgatatacg gtttaaatat tattatttgt caaattaagc gagacaaaac tttccattta
      481 cgtaa
//
LOCUS       DARBET010000231          465 bp    DNA     linear   BCT 07-NOV-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4027
            SAMEA114569841-rid20112183.denovo.242, whole genome shotgun
            sequence.
ACCESSION   DARBET010000231 DARBET010000000
VERSION     DARBET010000231.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA114569841
            Sequence Read Archive: ERR12203666
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 465)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 465)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001972618.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001972618.1
            Genome Coverage       :: 132x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/07/2023 13:18:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,041
            CDSs (total)                      :: 2,975
            Genes (coding)                    :: 2,908
            CDSs (with protein)               :: 2,908
            Genes (RNA)                       :: 66
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 67
            CDSs (without protein)            :: 67
            Pseudo Genes (ambiguous residues) :: 0 of 67
            Pseudo Genes (frameshifted)       :: 26 of 67
            Pseudo Genes (incomplete)         :: 28 of 67
            Pseudo Genes (internal stop)      :: 20 of 67
            Pseudo Genes (multiple problems)  :: 6 of 67
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..465
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA114569841-rid20112183.denovo.242"
                     /isolate="VRE4027"
                     /db_xref="taxon:1352"
                     /country="Denmark"
     gene            124..327
                     /locus_tag="SCJ86_002966"
     CDS             124..327
                     /locus_tag="SCJ86_002966"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296753.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="leucocin A/sakacin P family class II
                     bacteriocin"
                     /protein_id="HEG1170435.1"
                     /translation="MKHCVILGILGTCLAGIGTGIDVDAATYYGNGLYCNKEKCWVNW
                     GQSWSEGLKRWGDNLFGSFSGGR"
BASE COUNT          190 a           47 c           86 g          142 t
ORIGIN      
        1 tcaatcattg ttatataata aaatgttata taataaaatt actaatgata tttaatatta
       61 caagcaatct taagaaattc gaaaaggaaa aaatgattat gaaaaagaaa gaaaaagaaa
      121 gtattgaaac attgtgttat tctaggaata ttaggaactt gtctagctgg cattggtaca
      181 ggaatagacg ttgacgcagc tacttactat ggaaatggtc tttattgtaa caaagaaaaa
      241 tgttgggtaa attggggaca atcatggtca gaagggttaa aacgttgggg agataatctt
      301 tttggtagtt ttagtggagg acgataatga cttcaataaa cattgatcac acaaaaaaag
      361 tcatacatga attatacaat agtttaatga cacgacctaa tcagacgaaa gcattattag
      421 atattactga tgttttattt caggtatata aaaaaattga tacag
//
LOCUS       DARBET010000232          422 bp    DNA     linear   BCT 07-NOV-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4027
            SAMEA114569841-rid20112183.denovo.243, whole genome shotgun
            sequence.
ACCESSION   DARBET010000232 DARBET010000000
VERSION     DARBET010000232.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA114569841
            Sequence Read Archive: ERR12203666
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 422)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 422)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001972618.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001972618.1
            Genome Coverage       :: 132x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/07/2023 13:18:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,041
            CDSs (total)                      :: 2,975
            Genes (coding)                    :: 2,908
            CDSs (with protein)               :: 2,908
            Genes (RNA)                       :: 66
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 67
            CDSs (without protein)            :: 67
            Pseudo Genes (ambiguous residues) :: 0 of 67
            Pseudo Genes (frameshifted)       :: 26 of 67
            Pseudo Genes (incomplete)         :: 28 of 67
            Pseudo Genes (internal stop)      :: 20 of 67
            Pseudo Genes (multiple problems)  :: 6 of 67
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..422
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA114569841-rid20112183.denovo.243"
                     /isolate="VRE4027"
                     /db_xref="taxon:1352"
                     /country="Denmark"
     gene            complement(<1..132)
                     /locus_tag="SCJ86_002967"
     CDS             complement(<1..132)
                     /locus_tag="SCJ86_002967"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014748673.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS110 family transposase"
                     /protein_id="HEG1170436.1"
                     /translation="MKLFVGIDVSSEKLDVCFLTDDNQLSILSEISVANDIEGASFIR
                     "
BASE COUNT          144 a           82 c           83 g          113 t
ORIGIN      
        1 tcgtatgaat gaagcacctt cgatgtcatt ggcaacagag atttcagata agatagacaa
       61 ttgattgtcg tctgttaaaa aacaaacatc tagtttttca gagctaacgt caataccgac
      121 aaataatttc atggaagaag gacctccttt cgatataaat tttggaaaat gcttcgatac
      181 tgtgggattt cccagaaact cactgtatag ccacaacctc gcctaataga atacggtgat
      241 aaaagagcta gaagctgcct tccaaactac tatttgaaag tctagtccat tatcgaaaca
      301 gctaatgagt tggtagtgat aacagtggtt cgggtaacag actaaacagg gtagacaaac
      361 gctacaggag gaaatagcaa attcccgttg gatcctttcc atacatccat tgtactctga
      421 ga
//
LOCUS       DARBET010000233          401 bp    DNA     linear   BCT 07-NOV-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4027
            SAMEA114569841-rid20112183.denovo.244, whole genome shotgun
            sequence.
ACCESSION   DARBET010000233 DARBET010000000
VERSION     DARBET010000233.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA114569841
            Sequence Read Archive: ERR12203666
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 401)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 401)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001972618.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001972618.1
            Genome Coverage       :: 132x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/07/2023 13:18:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,041
            CDSs (total)                      :: 2,975
            Genes (coding)                    :: 2,908
            CDSs (with protein)               :: 2,908
            Genes (RNA)                       :: 66
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 67
            CDSs (without protein)            :: 67
            Pseudo Genes (ambiguous residues) :: 0 of 67
            Pseudo Genes (frameshifted)       :: 26 of 67
            Pseudo Genes (incomplete)         :: 28 of 67
            Pseudo Genes (internal stop)      :: 20 of 67
            Pseudo Genes (multiple problems)  :: 6 of 67
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..401
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA114569841-rid20112183.denovo.244"
                     /isolate="VRE4027"
                     /db_xref="taxon:1352"
                     /country="Denmark"
     gene            99..293
                     /locus_tag="SCJ86_002968"
     CDS             99..293
                     /locus_tag="SCJ86_002968"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292375.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEG1170437.1"
                     /translation="MYYVEVKTKGVKNKQYVKGISNEYPLLGSWKEAAPFSKPCAIKI
                     KNELEKELTCGKAVVDIIEK"
BASE COUNT          177 a           51 c           68 g          105 t
ORIGIN      
        1 tgattctttt tatttatcat aaagatatag atacataatc attttcactt acatatattt
       61 gaatattaac taaaaacagc aagaaagaag aggatcaaat gtattatgta gaagtaaaaa
      121 caaaaggcgt caaaaacaaa caatatgtga aaggtatcag taacgaatat cctttacttg
      181 gttcatggaa agaagctgct cctttttcca aaccatgtgc cataaaaata aagaacgagc
      241 tagaaaaaga actgacttgt ggtaaagctg ttgtagatat catcgaaaag taaatccaat
      301 gtatagaaag gaagatcaca catgaaaagc gacgtacaaa ttgctaagat tatagaatta
      361 gaagaagaag acgcagtgtt gacaattgaa aaaaaataaa t
//
LOCUS       DARBET010000234          348 bp    DNA     linear   BCT 07-NOV-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4027
            SAMEA114569841-rid20112183.denovo.245, whole genome shotgun
            sequence.
ACCESSION   DARBET010000234 DARBET010000000
VERSION     DARBET010000234.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA114569841
            Sequence Read Archive: ERR12203666
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 348)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 348)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001972618.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001972618.1
            Genome Coverage       :: 132x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/07/2023 13:18:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,041
            CDSs (total)                      :: 2,975
            Genes (coding)                    :: 2,908
            CDSs (with protein)               :: 2,908
            Genes (RNA)                       :: 66
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 67
            CDSs (without protein)            :: 67
            Pseudo Genes (ambiguous residues) :: 0 of 67
            Pseudo Genes (frameshifted)       :: 26 of 67
            Pseudo Genes (incomplete)         :: 28 of 67
            Pseudo Genes (internal stop)      :: 20 of 67
            Pseudo Genes (multiple problems)  :: 6 of 67
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..348
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA114569841-rid20112183.denovo.245"
                     /isolate="VRE4027"
                     /db_xref="taxon:1352"
                     /country="Denmark"
     gene            111..>348
                     /locus_tag="SCJ86_002969"
     CDS             111..>348
                     /locus_tag="SCJ86_002969"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611882.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS982 family transposase"
                     /protein_id="HEG1170438.1"
                     /translation="MRSSKYTEHYTDTFITFKEIMRTVSNIYHNCVPDKIKNRRNTDQ
                     LKQRDTVIIACVIWGIINGYTSQRATYRAVCSVLF"
BASE COUNT          127 a           60 c           60 g          101 t
ORIGIN      
        1 cccgtggtgc tagttaattt tttcctagag gaaaatgtct aaacatgttg gtaaatcaac
       61 aaaaaaacgc atatagtagt ctcaaaactc ccaagaaatg aggactacat atgcgttcat
      121 caaaatatac tgaacattat acagatactt ttataacatt caaggaaatt atgagaactg
      181 tttctaacat ataccacaat tgtgtaccag ataagattaa aaaccgaaga aatactgatc
      241 aactgaagca acgcgataca gtgattattg cttgtgttat atggggcata attaatggct
      301 atactagcca aagagccacg tatagagcgg tttgttccgt cttatttc
//
LOCUS       DARBET010000235          345 bp    DNA     linear   BCT 07-NOV-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4027
            SAMEA114569841-rid20112183.denovo.246, whole genome shotgun
            sequence.
ACCESSION   DARBET010000235 DARBET010000000
VERSION     DARBET010000235.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA114569841
            Sequence Read Archive: ERR12203666
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 345)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 345)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001972618.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001972618.1
            Genome Coverage       :: 132x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/07/2023 13:18:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,041
            CDSs (total)                      :: 2,975
            Genes (coding)                    :: 2,908
            CDSs (with protein)               :: 2,908
            Genes (RNA)                       :: 66
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 67
            CDSs (without protein)            :: 67
            Pseudo Genes (ambiguous residues) :: 0 of 67
            Pseudo Genes (frameshifted)       :: 26 of 67
            Pseudo Genes (incomplete)         :: 28 of 67
            Pseudo Genes (internal stop)      :: 20 of 67
            Pseudo Genes (multiple problems)  :: 6 of 67
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..345
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA114569841-rid20112183.denovo.246"
                     /isolate="VRE4027"
                     /db_xref="taxon:1352"
                     /country="Denmark"
     gene            complement(<1..321)
                     /locus_tag="SCJ86_002970"
     CDS             complement(<1..321)
                     /locus_tag="SCJ86_002970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000997695.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS256 family transposase"
                     /protein_id="HEG1170439.1"
                     /translation="MNDFTTEIVQTLVTKGDLNELFRSHLEKAINTLLRTELTAFLDY
                     EKYDRTGFNSGNSRNGSYFRSIKTEYGELTLEIPRDRNGEFKQQTLPAYKRTNDTLET
                     TIIHL"
BASE COUNT           97 a           55 c           63 g          130 t
ORIGIN      
        1 taaatggata atagtggttt ccaatgtatc gtttgttctt ttgtaggctg gtaaagtttg
       61 ttgtttaaac tcaccattac gatctctagg tatttccaat gttaattcac catattcggt
      121 tttgattgat cgaaagtaag aaccgtttct cgaattacct gaattaaaac cagtgcgatc
      181 atatttttcg taatctaaaa aagccgttaa ttcagtccgt aggagtgtgt ttatcgcttt
      241 ttctaagtgc gaacggaata attcatttaa atcgccttta gtgactagag tttgcacaat
      301 ttctgtagta aaatcattca tagggaagtc ctcttttctg tgaat
//
LOCUS       DARBET010000236          341 bp    DNA     linear   BCT 07-NOV-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4027
            SAMEA114569841-rid20112183.denovo.247, whole genome shotgun
            sequence.
ACCESSION   DARBET010000236 DARBET010000000
VERSION     DARBET010000236.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA114569841
            Sequence Read Archive: ERR12203666
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 341)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 341)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001972618.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001972618.1
            Genome Coverage       :: 132x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/07/2023 13:18:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,041
            CDSs (total)                      :: 2,975
            Genes (coding)                    :: 2,908
            CDSs (with protein)               :: 2,908
            Genes (RNA)                       :: 66
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 67
            CDSs (without protein)            :: 67
            Pseudo Genes (ambiguous residues) :: 0 of 67
            Pseudo Genes (frameshifted)       :: 26 of 67
            Pseudo Genes (incomplete)         :: 28 of 67
            Pseudo Genes (internal stop)      :: 20 of 67
            Pseudo Genes (multiple problems)  :: 6 of 67
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..341
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA114569841-rid20112183.denovo.247"
                     /isolate="VRE4027"
                     /db_xref="taxon:1352"
                     /country="Denmark"
     gene            <1..>341
                     /locus_tag="SCJ86_002971"
     CDS             <1..>341
                     /locus_tag="SCJ86_002971"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016250137.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEG1170440.1"
                     /translation="EATQPITADDTEFVSSATKLDDKSDADKYNPEGQKVTTELNKEP
                     DAFEGIKNKEDLPKDTKYTWKEKVDVSAAGNKKGTVVVTYPDGSSDEVEVDVTVTDNR
                     SDADKYEPTVEG"
BASE COUNT          141 a           50 c           76 g           74 t
ORIGIN      
        1 gaagcaactc aaccaattac agcagatgat acagaatttg tatcaagtgc aacaaaactg
       61 gatgataaat ctgacgctga caaatataat cctgaaggtc aaaaagtgac tacagaattg
      121 aataaggaac cagacgcatt tgagggaatt aagaacaaag aagatttacc aaaagatact
      181 aagtatactt ggaaagaaaa agtagatgtt agcgcagctg gaaataaaaa agggactgtt
      241 gtagtgacat atccagatgg atcatctgat gaagttgaag tagatgttac agtgacagac
      301 aatcgttctg acgccgataa atatgagcca acagtagaag g
//
LOCUS       DARBET010000237          338 bp    DNA     linear   BCT 07-NOV-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4027
            SAMEA114569841-rid20112183.denovo.248, whole genome shotgun
            sequence.
ACCESSION   DARBET010000237 DARBET010000000
VERSION     DARBET010000237.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA114569841
            Sequence Read Archive: ERR12203666
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 338)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 338)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001972618.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001972618.1
            Genome Coverage       :: 132x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/07/2023 13:18:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,041
            CDSs (total)                      :: 2,975
            Genes (coding)                    :: 2,908
            CDSs (with protein)               :: 2,908
            Genes (RNA)                       :: 66
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 67
            CDSs (without protein)            :: 67
            Pseudo Genes (ambiguous residues) :: 0 of 67
            Pseudo Genes (frameshifted)       :: 26 of 67
            Pseudo Genes (incomplete)         :: 28 of 67
            Pseudo Genes (internal stop)      :: 20 of 67
            Pseudo Genes (multiple problems)  :: 6 of 67
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..338
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA114569841-rid20112183.denovo.248"
                     /isolate="VRE4027"
                     /db_xref="taxon:1352"
                     /country="Denmark"
     gene            1..338
                     /locus_tag="SCJ86_002972"
                     /pseudo
     CDS             1..338
                     /locus_tag="SCJ86_002972"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_086558187.1"
                     /note="frameshifted; incomplete; missing N-terminus and
                     C-terminus; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
BASE COUNT          142 a           59 c           64 g           73 t
ORIGIN      
        1 gtaaagcaat ctaaaacgag tggttcattc aaagaaaaag ataatcttac gcctgaacaa
       61 aaagaattgt tagaactacg taaaagaaac cagcaattag aaatggaaaa tgatatttta
      121 aagcaagcag cgctgatatt cggacgaaga gacaagtaat cgatgcgaat aagcatcttt
      181 accctatatc agcgatgtgc agaatattag gtctatcacg tcagtcctat tattatcaat
      241 caaaaccaaa gaaagacgaa tcagaacttg aagaagtagt cgctgaagaa tttatccgca
      301 gccgaaaggc ctacggctca agaaaaataa aaaaagcc
//
LOCUS       DARBET010000238          331 bp    DNA     linear   BCT 07-NOV-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4027
            SAMEA114569841-rid20112183.denovo.249, whole genome shotgun
            sequence.
ACCESSION   DARBET010000238 DARBET010000000
VERSION     DARBET010000238.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA114569841
            Sequence Read Archive: ERR12203666
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 331)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 331)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001972618.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001972618.1
            Genome Coverage       :: 132x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/07/2023 13:18:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,041
            CDSs (total)                      :: 2,975
            Genes (coding)                    :: 2,908
            CDSs (with protein)               :: 2,908
            Genes (RNA)                       :: 66
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 67
            CDSs (without protein)            :: 67
            Pseudo Genes (ambiguous residues) :: 0 of 67
            Pseudo Genes (frameshifted)       :: 26 of 67
            Pseudo Genes (incomplete)         :: 28 of 67
            Pseudo Genes (internal stop)      :: 20 of 67
            Pseudo Genes (multiple problems)  :: 6 of 67
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..331
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA114569841-rid20112183.denovo.249"
                     /isolate="VRE4027"
                     /db_xref="taxon:1352"
                     /country="Denmark"
     gene            <1..>331
                     /locus_tag="SCJ86_002973"
     CDS             <1..>331
                     /locus_tag="SCJ86_002973"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012477736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="IS6-like element ISS1D family transposase"
                     /protein_id="HEG1170441.1"
                     /translation="YLWKKKNRQSFYSWKMDETYIKIKGRWHYLYRAIDADGLTLDIW
                     LRKKRDTQAAYAFLKRLHKQFGEPKAIVTDKAPSLGSAFRKLQSVGLYTKTEHRTVKY
                     LNNLIEQ"
BASE COUNT          114 a           60 c           68 g           89 t
ORIGIN      
        1 attatctttg gaagaagaaa aatagacaat ccttctattc atggaaaatg gacgaaacct
       61 atatcaaaat taagggacgt tggcattatc tttatcgtgc aattgatgcg gacggcttaa
      121 ccttagatat ctggttacga aagaaacggg atacgcaagc agcctatgct ttcttaaaac
      181 gactccataa acagtttggt gagccgaaag caattgtgac cgataaagca ccttctcttg
      241 gctccgcctt tagaaagtta cagagtgtgg gtttatatac taagacagag caccgaactg
      301 tgaagtatct taacaattta atagaacaag a
//
LOCUS       DARBET010000239          321 bp    DNA     linear   BCT 07-NOV-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4027
            SAMEA114569841-rid20112183.denovo.250, whole genome shotgun
            sequence.
ACCESSION   DARBET010000239 DARBET010000000
VERSION     DARBET010000239.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA114569841
            Sequence Read Archive: ERR12203666
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 321)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 321)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001972618.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001972618.1
            Genome Coverage       :: 132x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/07/2023 13:18:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,041
            CDSs (total)                      :: 2,975
            Genes (coding)                    :: 2,908
            CDSs (with protein)               :: 2,908
            Genes (RNA)                       :: 66
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 67
            CDSs (without protein)            :: 67
            Pseudo Genes (ambiguous residues) :: 0 of 67
            Pseudo Genes (frameshifted)       :: 26 of 67
            Pseudo Genes (incomplete)         :: 28 of 67
            Pseudo Genes (internal stop)      :: 20 of 67
            Pseudo Genes (multiple problems)  :: 6 of 67
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..321
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA114569841-rid20112183.denovo.250"
                     /isolate="VRE4027"
                     /db_xref="taxon:1352"
                     /country="Denmark"
     gene            complement(69..321)
                     /locus_tag="SCJ86_002974"
                     /pseudo
     CDS             complement(69..321)
                     /locus_tag="SCJ86_002974"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298938.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
BASE COUNT           79 a           78 c           51 g          113 t
ORIGIN      
        1 tccaaactcg taactcgagc ttggaacagc tttttatcta cttcgtttcg aatttttgaa
       61 caaaaaaata aaggctcctt gcctttccat agccaggtgg ctaatttctt taaattcatt
      121 gcagcaaaca ctagcatcgt atgcgtcgcg actttttcca agcctcgata cttggtccaa
      181 cgcatcccat gcttttcttt tgcatccgca aatattcgtt caatcgtttg tttccgtttt
      241 ttgtaaattg atttattcag atctgttagg cgaagatgtt ccacttcatc catcagatct
      301 ccccaaacat gtctcgtgat g
//
LOCUS       DARBET010000240          308 bp    DNA     linear   BCT 07-NOV-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4027
            SAMEA114569841-rid20112183.denovo.251, whole genome shotgun
            sequence.
ACCESSION   DARBET010000240 DARBET010000000
VERSION     DARBET010000240.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA114569841
            Sequence Read Archive: ERR12203666
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 308)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 308)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001972618.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001972618.1
            Genome Coverage       :: 132x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/07/2023 13:18:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,041
            CDSs (total)                      :: 2,975
            Genes (coding)                    :: 2,908
            CDSs (with protein)               :: 2,908
            Genes (RNA)                       :: 66
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 67
            CDSs (without protein)            :: 67
            Pseudo Genes (ambiguous residues) :: 0 of 67
            Pseudo Genes (frameshifted)       :: 26 of 67
            Pseudo Genes (incomplete)         :: 28 of 67
            Pseudo Genes (internal stop)      :: 20 of 67
            Pseudo Genes (multiple problems)  :: 6 of 67
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..308
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA114569841-rid20112183.denovo.251"
                     /isolate="VRE4027"
                     /db_xref="taxon:1352"
                     /country="Denmark"
     gene            complement(76..>308)
                     /locus_tag="SCJ86_002975"
     CDS             complement(76..>308)
                     /locus_tag="SCJ86_002975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HEG1170442.1"
                     /translation="LKNLPETLDAQLRTKLQNLLTYEEGIYNAMIYPYSNGKIEAKIP
                     HIKTLKRLSYGFKSFENMKIRIFLINQLIQVK"
BASE COUNT           94 a           46 c           56 g          112 t
ORIGIN      
        1 atgttggtga agttcaccaa catcaaaaag tatagagccg atagaaggac tgaccttctg
       61 tctcagattt ttttgttatt tcacttgaat caattgattg atcaaaaaaa tcctgatttt
      121 catgttctca aatgatttaa atccataaga taatcgtttc agtgtcttga tatggggaat
      181 cttggcctct attttgccat tagaatacgg gtaaatcata gcattgtaaa taccttcttc
      241 atacgttaaa aggttttgaa gtttcgtgcg aagttgtgca tcaagtgttt caggaagatt
      301 ttttaata
//
LOCUS       DARBET010000241          283 bp    DNA     linear   BCT 07-NOV-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4027
            SAMEA114569841-rid20112183.denovo.253, whole genome shotgun
            sequence.
ACCESSION   DARBET010000241 DARBET010000000
VERSION     DARBET010000241.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA114569841
            Sequence Read Archive: ERR12203666
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 283)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 283)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001972618.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001972618.1
            Genome Coverage       :: 132x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/07/2023 13:18:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,041
            CDSs (total)                      :: 2,975
            Genes (coding)                    :: 2,908
            CDSs (with protein)               :: 2,908
            Genes (RNA)                       :: 66
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 67
            CDSs (without protein)            :: 67
            Pseudo Genes (ambiguous residues) :: 0 of 67
            Pseudo Genes (frameshifted)       :: 26 of 67
            Pseudo Genes (incomplete)         :: 28 of 67
            Pseudo Genes (internal stop)      :: 20 of 67
            Pseudo Genes (multiple problems)  :: 6 of 67
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..283
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA114569841-rid20112183.denovo.253"
                     /isolate="VRE4027"
                     /db_xref="taxon:1352"
                     /country="Denmark"
     gene            1..283
                     /locus_tag="SCJ86_002976"
                     /pseudo
     CDS             1..283
                     /locus_tag="SCJ86_002976"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611768.1"
                     /note="frameshifted; incomplete; missing N-terminus and
                     C-terminus; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=3
                     /transl_table=11
                     /product="transposase"
BASE COUNT          109 a           57 c           56 g           61 t
ORIGIN      
        1 aacaaatcgt cgatctctat ctcgctggta agcctcgcgc agaaattatt cgagagtatg
       61 agcttacgcc ttcttctttc gataaatgga tgaagcaagc acaatcaacg ggctcattca
      121 aagaaagaga caacttaaca ccagaacaag cagaattgat cgcactaaga aagaaaaata
      181 agcaactcga aatggagaat gatattttaa agcaagcggc gctgatattc ggacgaaaag
      241 acaagtaatt gatgccaaca agcataaata ttccatatca gcg
//
LOCUS       DARBET010000242        19598 bp    DNA     linear   BCT 07-NOV-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4027
            SAMEA114569841-rid20112183.guided.048, whole genome shotgun
            sequence.
ACCESSION   DARBET010000242 DARBET010000000
VERSION     DARBET010000242.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA114569841
            Sequence Read Archive: ERR12203666
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 19598)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 19598)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001972618.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001972618.1
            Genome Coverage       :: 132x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/07/2023 13:18:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,041
            CDSs (total)                      :: 2,975
            Genes (coding)                    :: 2,908
            CDSs (with protein)               :: 2,908
            Genes (RNA)                       :: 66
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 67
            CDSs (without protein)            :: 67
            Pseudo Genes (ambiguous residues) :: 0 of 67
            Pseudo Genes (frameshifted)       :: 26 of 67
            Pseudo Genes (incomplete)         :: 28 of 67
            Pseudo Genes (internal stop)      :: 20 of 67
            Pseudo Genes (multiple problems)  :: 6 of 67
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19598
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA114569841-rid20112183.guided.048"
                     /isolate="VRE4027"
                     /db_xref="taxon:1352"
                     /country="Denmark"
     gene            complement(52..1092)
                     /locus_tag="SCJ86_002977"
     CDS             complement(52..1092)
                     /locus_tag="SCJ86_002977"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000288739.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor G"
                     /protein_id="HEG1170443.1"
                     /translation="MYTSINGELCKIDRAYSGEIVILQNEFLKLNSVLGDTKLLPQRK
                     KIENPHPLLQTTVEPSKPEQREMLLDALLEISDSDPLLRYYVDSTTHEIILSFLGKVQ
                     MEVISALLQEKYHVEIELKEPTVIYMERPLKNAEYTIHIEVPPNPFWASIGLSVSPLP
                     LGSGMQYESSVSLGYLNQSFQNAVMEGIRYGCEQGLYGWNVTDCKICFKYGLYYSPVS
                     TPADFRMLTPIVLEQAFRKAGTELLEPYLSFKVYAPQEYLSRAYNDAPKYCANIVNTQ
                     LKNNEVIIIGEIPARCIQDYRNDLTFFTNGLSVCLAELKGYQVTTGEPVCQTRRLNSR
                     IDKVRYMFNKIT"
     gene            1454..1951
                     /gene="dfrG"
                     /locus_tag="SCJ86_002978"
     CDS             1454..1951
                     /gene="dfrG"
                     /locus_tag="SCJ86_002978"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000868795.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trimethoprim-resistant dihydrofolate reductase
                     DfrG"
                     /protein_id="HEG1170444.1"
                     /translation="MKVSLIAAMDKNRVIGKENDIPWRIPKDWEYVKNTTKGHPIILG
                     RKNLESIGRALPDRRNIILTRDKGFTFNGCEIVHSIEDVFELCKNEEEIFIFGGEQIY
                     NLFFPYVEKMYITKIHHEFEGDTFFPEVNYEEWNEVFAQKGIKNDKNPYNYYFHVYER
                     KNLLS"
     gene            complement(2023..3975)
                     /locus_tag="SCJ86_002979"
     CDS             complement(2023..3975)
                     /locus_tag="SCJ86_002979"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017431446.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="insertion element protein"
                     /protein_id="HEG1170445.1"
                     /translation="MTKLKRLATKEDEIVDVKIPISNEELKDRAKQYDLLTPKRFATR
                     YNKMLFLPTSFKWNGSEYPIQYNYCINPFCCNFGKEQHKFKDVKGKPSRYKMTGSSKD
                     KGHKGMYCNDNPIGRGVSQNCTVTPLSNWSVVEEIKRLIEINSIQDVEPDYQFHKEGC
                     SEEESTPFNEPKQFYKRGKSRGKSQRYQCKACKKFTNVLPKREETTTYHQQKNTILPM
                     FAKMVVGRVSVSRTCDILGIGVGTYYHKLEWLYRRCLEFLERYETQPLQTKKFNEMWL
                     NTDKMHYYLNNVRKKGQGSKKYTGFEDLNMQTYIVVSAEVLSRYVFRSDVAYDWNISM
                     DELNEDTRKFKEDHLNTFSRKNDRLDWSYYPQEPSANDSENRNAYLHELGKITNRSRF
                     VDGLNVDAPYTTTAHYWLIKQMVNADEWRMISDDDFSIRNAFYRVFTKELRLSDAHHF
                     ICQVNKTKSRKQCLKEFGQAKAELLDWGDIRGFKTKFLRTLASHYLTELLTSHQFHEE
                     AISKDGERYRKYADNPIKHPLATKDKGFYSVDCRTDLSALEPNEIAKMLLNVNDHSTN
                     SFIQQIRRYISSLERPLTTARGDKKSYIYANFNPKYAQFAITILRTYYNFCRPFKSAD
                     KKVLTPAQRLGITDKQFDWKDIIYFK"
     gene            complement(3982..4218)
                     /locus_tag="SCJ86_002980"
     CDS             complement(3982..4218)
                     /locus_tag="SCJ86_002980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001159903.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEG1170446.1"
                     /translation="MQFLEPKNKNAKSVDWEISEQVRVIVKQYAEYAERTESEAVDEF
                     LLNILDDKKFIEWIANKRSNKRIVEKMGIKDRVG"
     gene            complement(4453..5256)
                     /locus_tag="SCJ86_002981"
     CDS             complement(4453..5256)
                     /locus_tag="SCJ86_002981"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017644183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTP-binding protein"
                     /protein_id="HEG1170447.1"
                     /translation="MKIINIGVLAHVDAGKTTLTESLLYNSGAITELGSVDKGTTRTD
                     NTLLERQRGITIQTGITSFQWENTKVNIIDTPGHMDFLAEVYRSLSVLDGAILLISAK
                     DGVQAQTRILFHALRKMGIPTIFFINKIDQNGIDLSTVYQDIKEKLSAEIVIKQKVEL
                     YPNMCVTNFTESEQWDTVIEGNDDLLEKYMSGKSLEALELEQEESIRFQNCSLFPLYH
                     GSAKSNIGIDNLIEVITNKFYSSTHRGPSELCGNVFKIEYTKKRQRLAY"
     gene            complement(5272..5388)
                     /locus_tag="SCJ86_002982"
     CDS             complement(5272..5388)
                     /locus_tag="SCJ86_002982"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001814923.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetracycline resistance determinant leader
                     peptide"
                     /protein_id="HEG1170448.1"
                     /translation="MILKYPENICMLCIPMVMHKNPSDKSIYHWDFYALLGF"
     gene            complement(5633..6562)
                     /locus_tag="SCJ86_002983"
     CDS             complement(5633..6562)
                     /locus_tag="SCJ86_002983"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017650613.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein"
                     /protein_id="HEG1170449.1"
                     /translation="MIIKIERKEKKEKPKKSKKPSKQRKVPMIKVGTHKKLTFVLWVL
                     LIGSVGFGIYKNFTAIDTHTVRETEIIKQQIVDTNQVESFVKSFAKDYFSWQQSQEAI
                     DKRNEKLTHYLTEELQVLNEEMIRKDIPTSSSVNDIQVWQVSQVNENTFEVLFSVEQV
                     ITEDKDKETISSSFHVVVHIDESDNMVIIKNPTMSKKPQKSDYQPKQLESDHTVDTET
                     MDEITSFLETFFQLYPTATEKELTYYVSNHVLPMINKEYVFEELVNPIFTRKDNQVIV
                     NVAVKYLDQETKATQISQFELILEKQDNWKIVK"
     gene            complement(6579..7601)
                     /locus_tag="SCJ86_002984"
     CDS             complement(6579..7601)
                     /locus_tag="SCJ86_002984"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000768374.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional lysozyme/C40 family peptidase"
                     /protein_id="HEG1170450.1"
                     /translation="MKLKITVICSVVFSLFFFLLLFLVIFFADDTDSGENNKDSSIPQ
                     GGVTVSPEVLAHRPLIEKYGKEYGIEDYVSYILAIMQVESGGTAEDVMQSSESLGLPP
                     NSLSTEESIKQGVKYFSELLTSAEQQGVDIDSVIQSYNYGGGFLNYVRSHGKKYTYEL
                     AEQFSKEKSGGQKADYPNPIAIPVNGGWRYNYGNQFYVQLVSQYLTDTSPTEFDDETV
                     QVIMDEALKYEGFPYVFGGASPTTSFDCSGLIQWVYDKAGISLPRVAQDQYDATQEIS
                     MEEAQAGDLIFFHSTYNAGTYVTHVAIYLEGNRFYHAGDPIGYGDLSSRYWQDHLIGA
                     RRVIHN"
     gene            complement(7598..9625)
                     /locus_tag="SCJ86_002985"
     CDS             complement(7598..9625)
                     /locus_tag="SCJ86_002985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000192391.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEG1170451.1"
                     /translation="MTQKQKKFLHYSWITLLCVGVILLLLGTLGNAVQATGLVDETID
                     TSNEYSKYGLNQYQLDYYVDNSWGWLPWNWSDGIGQSVMYGLYAITNFIWTISLYLSN
                     ATGYLVKEAYALDFISQTSQAIGENMQTIAGITPNGFSSSGFYVGFLLLFILIVGIYV
                     AYTGLVKRETTKVIRAVLNFVVVFILSASFIAYAPDYIAKINDFSKDVSTASLNIGTK
                     IVLPNTDSQGKDSVDMIRDSLFSVQVKQPWLLLQYGSTDVEALGEERVESLLSTSPDT
                     NNGEDRENVVIEEIEDHDNTYLTLPKTISRLGTVFFLFVFNIGISIFVFLLTGIMIFS
                     QVLFIIFAMFLPISFLLSMLPSFDGMGRHAITKLFNVIMTRAGITLIITVAFSISTML
                     YSLSASSPFFMIMFLQIVTFAGIYFKLGDLMSLFTLQSNDSQNMGRQVFRRPRQMMNR
                     QTRRFQRSMSKIFRGKAGSTKQKTMTTKQNQTNKANHTRQNERTTPKKETALSNVKKQ
                     QGGIGQRLGEKTAHVLDTKNRLVNKRKQAKQQIQATPTHVKYALHQGKENVTHNVTDF
                     KQSMADTKAQRKQQRMNQQEQKRKNIAQKRLELDKAKEKRRKENPSSPVQNNQQHVRK
                     RQQTVTPVLSQNTKRENHQRPLKKEQKQKTPPKQFNQRKLNKRTDKRGQQT"
     gene            complement(9622..12075)
                     /locus_tag="SCJ86_002986"
     CDS             complement(9622..12075)
                     /locus_tag="SCJ86_002986"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000331166.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HEG1170452.1"
                     /translation="MAYPIKYMEDNLVFNHDGEVFAYYELIPYNYSFLSSDEKIQVHD
                     NFCQLIAQNRDGKIHALQLATESSIRATQEQSKKEITGRLKEIAYEKIDEQTEALISM
                     IGDNQVDYRFFIGFKLYLNEQEVSVKNVGKEIQKAFAEFFHGVNHKLMGDFVSMPMAE
                     IERFSKMEQLLESKLSRRFQIRKLEKDDFGYIIEHLYGQTGVPFDDYDYHLPKRYEDK
                     KVLVKKYDILKPTRCLIEENQRYLRIEQEDTTTYVAYFTINSIVGELDFPNSEIFYYQ
                     QQQFTFPVDTSMNVEIVANKKALSTVRNKKKELKDLDSHAWESDNETSTNVVDALESV
                     DELESNLDQTKESMYKLSYVVRVSAKNLDELKQRCDEVKDFYDDLSIKLVRPFGDMLG
                     LHNEFIPSSKRYINDYIQYVTSDFLAGLGFGATQMIGENSGIYVGYNLDTGRNIYLQP
                     SLASQGIKGSVTNALASAFIGSLGGGKSFSNNMLVYYSVLFGGQATIVDPKAERGEWK
                     ETLPDIAHEINIVNLTSENDNKGLLDPYVILKLPKDSESLAIDILTFLTGISSRDSEK
                     FPVLRKAIRSVTQSKQRGLLLVIDELRKEDTPISNSIADHIESFVDYDFAHLLFSDGS
                     VTQSISLEKQLNIIQVADLVLPDSNSSFEEYTTMELLSVAMLIVISTFALDFIHSDRS
                     IFKIVDLDEAWSFLQVAQGKALSMRLVRAGRAMNAGVYFVTQNADDLLDEKMKNNIGL
                     KFAFRSTDLVEIKKTLEFFGVDKEDENNQKRLRELENGQCLVSDIYGRVGVMQFHPIF
                     EELLHAFDTRPPVRNEVEE"
     gene            complement(12059..12454)
                     /locus_tag="SCJ86_002987"
     CDS             complement(12059..12454)
                     /locus_tag="SCJ86_002987"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_464632.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein"
                     /protein_id="HEG1170453.1"
                     /translation="MKKIRSYTSIWSVEKVIYAINDLQLPFPITFTQMTWFIVSLFVV
                     ILFGSLPPLSLIDGALLKYLGIPIVLTGFMCQKTFDGKKPYGFLKSIITYALRPKVTY
                     AGKQIRLKKETMNESITIVRSEQLDGISD"
     gene            complement(12526..13164)
                     /locus_tag="SCJ86_002988"
     CDS             complement(12526..13164)
                     /locus_tag="SCJ86_002988"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000248477.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEG1170454.1"
                     /translation="MVNVFENLKLLKLDMKDKGWVIDSFYFRYKQQNYIVLVKLFEKE
                     EKVPEYALLKLEFLKENDFSDMLAVYANSVKLYTDTKTIREYFGIEYSSNLGDVLFQF
                     SQTLARFIPTEVSEKKNEDQKEAMCFSLSQSDSEDPRKKYCFSVRRNPLKGNGELGKR
                     SPFNDNKTRLYRPSLYERLGSDTNLSFRYSMNPDDEETDEGIIAKWTKNKIE"
     gene            complement(13220..13714)
                     /locus_tag="SCJ86_002989"
     CDS             complement(13220..13714)
                     /locus_tag="SCJ86_002989"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000870467.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antirestriction protein ArdA"
                     /protein_id="HEG1170455.1"
                     /translation="MKVYVANLGRYNEGELVGAWFTPPIDEEEMAERIGLNEDYEEYA
                     IHDFELPFDVDEYTPISEINRLCEAIQEIEGTPIYNELKEIQGMWFSSLEELLENKED
                     IHCYSDCDSMEDVARYYVEETGQLGEVPSNLQNYIDYQSLGRDMEIEGNYLVTSHGVF
                     EYCQ"
     gene            complement(13781..14560)
                     /locus_tag="SCJ86_002990"
     CDS             complement(13781..14560)
                     /locus_tag="SCJ86_002990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000678782.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="abortive infection family protein"
                     /protein_id="HEG1170456.1"
                     /translation="MKGFMNMFTNDQFKSLLIKTLERKSSSNYYEGGNEIVSKMKISE
                     VTLDETDDFTYYKGYKKGWNTLCTYLKIRVPFDDLDFFESHKDLITQTASSIYDKQGD
                     NVLVDTILVPLPENYEVINFSQLKISDVVSQAIEDAESFMSNGEYQRAFDRVHTAFHG
                     YLIEILKKYEITVPRDENLSKLYSRIQQLIEKEIQPTELADIVKTTIRSSNGMISSLN
                     EARNRHSLAHPNTNIIGKREAKLIIGISSTVTDYISGYLDK"
     gene            complement(14602..14823)
                     /locus_tag="SCJ86_002991"
     CDS             complement(14602..14823)
                     /locus_tag="SCJ86_002991"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_464634.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEG1170457.1"
                     /translation="MNFGQNLYNWFLDNAQSLVLLAIVVIGLFLGFKREFSKLIGFLI
                     IALIAVGLVFNAAGVKDVLLNLFNRIIGA"
     gene            complement(14820..15110)
                     /locus_tag="SCJ86_002992"
     CDS             complement(14820..15110)
                     /locus_tag="SCJ86_002992"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000055367.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEG1170458.1"
                     /translation="MSLLTKELKKLGFQCGIEFQAYIQNTGKYTSLIIEGKRQAGDTI
                     YTYDFYKVRFYNNYTNRVTVYGEHLTPFQLLRRVKSYIYYREKYLKERRTIT"
     gene            complement(15107..16291)
                     /locus_tag="SCJ86_002993"
     CDS             complement(15107..16291)
                     /locus_tag="SCJ86_002993"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000426690.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation factor domain-containing
                     protein"
                     /protein_id="HEG1170459.1"
                     /translation="MENEKIWIKELKEKRLAYGVSQNKLAVASHITRPYLSDIETGKA
                     VPTQQVKEDLLNALERFNPDNPLEMLFDYVRIRFPTNDVAQIIGEVLRLNMDYMLHED
                     FGYYSYPEHYRFGDIVVLVSHDVSKGVLLELKGKGCRQFENFLLAQHRSWYDFFQDCM
                     EHKGIFKRLDLAINDKTGILNIPDLAKKCKQEECISVFRSFKNYRSGELVHRDEKPYM
                     GNTLYIGSLKSEVYFCIYEKDYEQYVKNDVPLEDAEVKNRFEIRLKNDRATQAMKDLL
                     AHQQAEKTAFEIINRYIRFADKDDTKRRSDWKTNERWEWFIGKNRGALRLTTQPEPYS
                     FERTLNWLHHQVAPTLKIASILDVLNGTTIISTMIQEAKLTEKHEKLIEQQHLAMEDL
                     IT"
     gene            complement(16473..17876)
                     /locus_tag="SCJ86_002994"
     CDS             complement(16473..17876)
                     /locus_tag="SCJ86_002994"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001130245.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF87 domain-containing protein"
                     /protein_id="HEG1170460.1"
                     /translation="MPLLHDRGERIKANDKHLVYHFVLISLLVVFLVVLLPFHWRALQ
                     SVNWQAVQLDTFFYQLHTPYFWSSILTALFVCGLISWLYYHYRIDSIKQLEHRQKLAR
                     MILENHWYEAEETQSEGFFKDLPSKKTKQRIRHFPKIYYRMKDGLIHIHVEITLGKYQ
                     DQLLHLENKLETGLYCELVEKTLKDSYVEYVLLYDTIGNRIGIEDVVAQDGRVKLMES
                     VYWEFDSLPHMLIAGGTGSGKSYFILTLIESLLHTNAKLYILDPKNADLADLGAVMNN
                     VYYRKEDMLACINQFYEDMIDRSETMKQHPNYKTGENYAYLGLSANFLIFDEYVAMMD
                     MLGRESTTVIHKLKQIVMLGRQAGFFLILACQRPDAKYFSDGIRDNFNFRVALGRMSE
                     MGFGMMFGSDTQKQFFLKPIKGRGYVDTGKSVISEFYTPLVPKRYDFLGEIGKVIQKK
                     QSEPVPHEVKGTGSDLL"
     gene            complement(17898..18671)
                     /locus_tag="SCJ86_002995"
     CDS             complement(17898..18671)
                     /locus_tag="SCJ86_002995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002388713.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEG1170461.1"
                     /translation="MTNTPLILKEIPKDEAISFIRQYHYSKILPRLCKYFLGIFSEEK
                     LLGVVELGWGTQPLQTIRKLFPDSSLQTTDYLEIGKMCFLPEMNQTNYFGSQALSALI
                     KWLKEHTDCHFLYTLADGIEGKCGYVYQASNFFYCGYFKTSVYRDKQSWEKIHPRSAR
                     LLLEENARFEQVEKKHWLSQAFCEYKGIEKINGRMFRYLYPLTKEAKKLLGHTLYRRH
                     YYPKEKNLRFEKRIAYQKYEAISQPTFDKQARIYNTQLF"
     gene            complement(18681..19058)
                     /locus_tag="SCJ86_002996"
     CDS             complement(18681..19058)
                     /locus_tag="SCJ86_002996"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001234189.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdcP family protein"
                     /protein_id="HEG1170462.1"
                     /translation="MRLAEGIVIEKDQTFGTLKFSALRREVRIQNEDGTVSEEIKERT
                     YDLKSKGQGRMIQVSIPASVPLKEFDYNAEVELINPVADTVATATFRGADVDWYVKAD
                     DLVLKGQTKPHQKYKKEASESEK"
     gene            complement(19079..19393)
                     /locus_tag="SCJ86_002997"
     CDS             complement(19079..19393)
                     /locus_tag="SCJ86_002997"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000421280.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdcP family protein"
                     /protein_id="HEG1170463.1"
                     /translation="MELKYVIPNMEKTFGQLEYAGEGNIEQRRINGRPTILSRSYNLY
                     STIQRADDIVVILPSEAGEKHFESDEKVKLINPHITAEGYKIGNRGFTNYIMHADDMV
                     KA"
BASE COUNT         5829 a         3818 c         3259 g         6692 t
ORIGIN      
        1 atttcatctt atttaataag aaaccatatt tatataacaa cataaaacgc actaagttat
       61 tttattgaac atatatctta ctttatctat ccgactattt agacgacggg tctggcaaac
      121 aggttcgcca gtggtaacct gatatccttt tagctctgct aaacaaacac taagcccatt
      181 tgtaaaaaaa gttaaatcat tgcgataatc ttgaatacat cgagcaggaa tttctccaat
      241 aataatgacc tcattatttt tcagttgagt atttacgata tttgcacaat atttgggagc
      301 atcgttatat gcccgtgaaa gatattcctg tggtgcataa actttaaaac taagatatgg
      361 ctctaacaat tctgttccag cttttctaaa ggcttgctcc agtacaatag gagtaagcat
      421 ccgaaaatct gctggagtac taacagggct atagtataaa ccgtacttaa aacagatttt
      481 acaatccgtc acattccaac catataatcc ttgttcgcaa ccatagcgta tcccttccat
      541 aactgcattt tgaaatgatt gatttaagta tccaagagaa accgagctct catactgcat
      601 tccacttccc aacggaagcg gtgatacaga taaaccaatg gaagcccaga aaggatttgg
      661 cggcacttcg atgtgaatgg tatattctgc attttttaac ggtctctcca tataaatgac
      721 tgtaggctct tttagttcta tctccacatg atacttttct tgcaacagtg cactaatcac
      781 ttccatttgt actttcccta agaaagaaag tataatttca tgtgtcgtag aatccacgta
      841 atatcgtaga agcggatcac tatctgagat ttccaaaagg gcatcaagca acatttctct
      901 ctgttcaggt ttactcggtt caacagttgt ttgtagtaga gggtgcggat tttcaatctt
      961 ttttctctgt ggcaatagtt ttgtatctcc aagaacacta tttaacttca aaaactcatt
     1021 ttgcaaaata acaatttctc cagaataagc tctatcaatc ttacataatt caccatttat
     1081 tgaagtatac atttctgtaa cttttatttt ttctttttct gatactctaa ccgaatctcg
     1141 taaatgtagt actccactat aaaggcgtat atatgcaagt aagtagttca gttttggagt
     1201 acaaaatgac aaaatgtact tcaaaatgga ctactttttt cattttaact tacataccaa
     1261 attacataca aaatgattta taaaatgcaa ttgaaaatga taattgaatg aaaatttgtg
     1321 attttgtggt attatgataa tataacttat agaaaaaatt gcttattcaa ctaacgggca
     1381 ggataatgag aaaaggtttt ttgatagaaa taagaaatta gatggtaaat tagttaaaaa
     1441 ggctgtgtta taaatgaaag tttctttgat tgctgcgatg gataagaata gagtgattgg
     1501 caaagagaat gacattcctt ggaggattcc caaggactgg gaatatgtta aaaatactac
     1561 aaagggacat ccgataatat taggtaggaa gaaccttgaa tcaatcggaa gagccttacc
     1621 tgacagaaga aatattattc tgacgagaga taaggggttt acctttaatg gttgtgaaat
     1681 tgttcattca atagaagatg tttttgagtt atgtaaaaac gaagaagaaa tttttatttt
     1741 cggaggagaa cagatttata atttgttttt cccttatgtt gagaaaatgt acatcacaaa
     1801 aatacatcat gaattcgaag gagatacttt ttttccagaa gtgaattatg aggaatggaa
     1861 tgaggtattt gcccaaaaag ggataaagaa tgataaaaat ccgtataact actattttca
     1921 tgtatatgaa agaaaaaact tattgagtta acgggtgcgt taattgaaga agaacagtaa
     1981 agataaagca caagagcttg ggattactcc aagctctttt tgtcatttga aataaattat
     2041 atctttccaa tcaaattgtt tatctgtaat ccctaatctt tgagcaggtg tcaaaacctt
     2101 tttatccgct gatttaaaag ggcgacaaaa gttgtagtaa gttcttagaa ttgtaatcgc
     2161 aaattgagca tacttcggat tgaagttagc gtaaatatag ctcttcttat caccacgagc
     2221 tgttgttaat ggtctttcaa gagatgatat ataacgtctt atctgttgaa taaagctatt
     2281 ggtcgaatgg tcattaacat ttaatagcat ctttgcgatt tcgtttggtt ccagagcaga
     2341 taaatccgtt ctacaatcaa ctgaataaaa tcctttatcc ttagtggcta atggatgttt
     2401 gatagggtta tcggcatatt tgcgatatct ttctccatct ttactaatag cctcttcgtg
     2461 gaattgatga cttgttagta attcagttag ataatgagat gccaaagtcc gtagaaactt
     2521 tgttttgaat cctctgatat ctccccaatc gagcagttct gcttttgctt gtccgaattc
     2581 ttttaagcat tgttttctgc ttttcgtctt gttaacttgg caaataaaat gatgtgcatc
     2641 gctcaacctt aattctttcg tgaatactct ataaaaggcg tttctaatag agaagtcatc
     2701 atcactaatc attcgccatt cgtcggcatt aaccatttgt ttaattaacc aatagtgagc
     2761 agttgtagta taaggagcat caacatttaa accatcgaca aatctactcc tattagtaat
     2821 tttccctaat tcgtgaagat aagcattcct attttcgcta tcgttagcgg atggttcttg
     2881 tggataataa gaccaatcta acctatcatt tttacgacta aaagtattga gatggtcttc
     2941 tttgaatttt cttgtatctt catttaattc atccattgaa atattccaat cataggcaac
     3001 atcagaacgg aacacatatc ttgataagac ctcagcagat acaactatat acgtttgcat
     3061 attaaggtct tcaaaccccg tatatttctt tgaaccttgt cctttcttac ggacgttatt
     3121 taggtagtag tgcatcttgt ccgtattaag ccacatctcg ttaaatttct ttgtttgaag
     3181 gggttgtgtc tcatatcgtt ctaaaaactc caaacaacgc ctatacagcc actccagctt
     3241 atgatagtag gttccaactc caattcctaa aatatcacaa gtacgactga ctgaaaccct
     3301 tccaacaacc atcttagcga acattggaag aatggtgttc ttttgttggt gataagtagt
     3361 ggtttcttct ctttttggta gaacattagt gaacttctta cacgccttac attgatagcg
     3421 ttgagatttg cccctacttt tccccctttt gtaaaattgc ttcggctcat tgaatggggt
     3481 tgattcttct tccgagcagc cttctttatg aaattgataa tctggctcaa catcttggat
     3541 gctattgatt tctataaggc gtttaatttc ttcaacaaca gaccaattag ataaaggggt
     3601 tactgtacag ttttgcgata cacctctgcc aataggattg tcattgcagt acataccttt
     3661 atgaccttta tctttggaac taccagtcat tttatatcga cttggtttcc cttttacatc
     3721 tttgaatttg tgctgttctt tcccaaaatt acaacaaaac ggattgatgc agtaattata
     3781 ttgaatggga tattcagaac cgttccattt aaaagacgtt ggcaagaaca acattttgtt
     3841 ataccttgtt gcaaatcgtt ttggagttaa taaatcatat tgtttagccc tatcttttaa
     3901 ttcttcgttt gaaatcggaa ttttaacatc tacaatttca tcttccttcg tggctaatct
     3961 tttaagcttg gtcattgtct atcaacccac tctatctttg atacccattt tttcaacaat
     4021 ccgcttatta cttcgcttgt tggcaatcca ttcaataaac ttcttatcgt ctaaaatgtt
     4081 caataagaat tcatccactg cttcgctttc agtacgctct gcatattcag cgtattgctt
     4141 aactatcacc cttacttgct ctgatatttc ccaatccaca gacttagcgt tcttattttt
     4201 tggctcaaga aattgcatag gaaaatgcac ccctttttgc ttagtaaatt gtaaccattt
     4261 taacacacca atttgcattc ctacaataat tatttcacgt tcgcattttt atatacattt
     4321 taacttacat tttgtggtgt aaattaacaa caaacgttga tttaacaata aaaaacgagt
     4381 atcaaattca gttgcaattt gatactcatt taggtttaca ttttcacttt ttgtactcca
     4441 aaactgaact acttaatatg caagacgttg tctttttttt gtatattcaa ttttgaaaac
     4501 atttccgcaa agttcagacg gacctcgatg tgttgatgaa taaaatttat tagtaataac
     4561 ttctataagg ttatcaatcc ctatattact ttttgcactt ccatgataaa gagggaacag
     4621 agaacaattc tgaaatctta tgctttcctc ttgttcgagt tccaatgctt ctaatgattt
     4681 accggacata tatttctcta aaaggtcatc gtttccctct attaccgtat cccattgttc
     4741 agattcggta aagttcgtca cacacatatt aggatacagt tctaccttct gtttgattac
     4801 aatttcggca gaaagtttct ctttaatatc ctgataaacc gttgataaat caattccatt
     4861 ttggtcaatc ttattgataa aaaagattgt gggaatcccc attttcctaa gtgcatgaaa
     4921 taatatacga gtttgtgctt gtacgccatc ttttgcagaa atcagtagaa ttgccccatc
     4981 taaaactgat aatgaacgat atacttctgc taagaaatcc atatgtcctg gcgtgtctat
     5041 gatgttcacc ttcgtatttt cccactgaaa agaggttatt cctgtctgaa ttgtaattcc
     5101 tctctgacgt tctaaaagcg tattatccgt cctcgttgta cctttgtcca cgcttcctaa
     5161 ttctgtaatc gctccactgt tatataataa gctttctgtt aaggtagttt ttcctgcatc
     5221 aacatgagct aaaactccaa tattaataat tttcatgtga ttttcctcca ttcaaaaacc
     5281 caaaagggca taaaaatccc agtgataaat acttttatca ctgggatttt tatgcataac
     5341 cataggtata caaagcatac agatattctc tggatacttt agaatcacat gataaaggta
     5401 ttcttaaact gggtacaaaa aactaagccc tcctaaaaaa ggacatctaa ttatttgttc
     5461 ccgctatcaa attgacagtt tatttaagaa taccttgccg catatttatt aactcctttt
     5521 aaatagtcac ttaaataata gcacgtaaga gcatatttgt aaaggaatct ccaatttttt
     5581 atcaaaaaga gtacatgatt acaaagtatc tgtaatcatg taccaatatt tgttatttta
     5641 caatcttcca attatcttgt ttttctaata tcaactcaaa ttgcgagatt tgggttgcct
     5701 ttgtttcttg atctaaatat ttcacagcga cattcacgat tacttggtta tcttttctgg
     5761 taaagatagg attcaccaat tcctcgaaaa catattcctt gttaatcata ggtaacacat
     5821 gattacttac atagtaagtc aattcttttt ctgtggcggt aggatagagc tggaagaacg
     5881 tttctaaaaa gctagtgatt tcgtccatcg tttctgtatc tactgtatgg tcactttcta
     5941 gctgtttggg ttggtagtcc gatttttgag gtttcttact cattgtcgga tttttgataa
     6001 taaccatatt atctgattca tctatatgga caaccacatg aaagctagac gatatagttt
     6061 ccttgtcttt gtcttcggta ataacttgtt caacagaaaa cagtacctca aaggtgttct
     6121 cattcacttg agaaacctgc cacacctgta tatcgttcac actagaacta gtaggaatat
     6181 ccttacgaat catttcttca tttaatacct gcaattcttc cgtcaaataa tgggtcaatt
     6241 tttcatttcg tttatcaatg gcttcttggg attgttgcca cgaaaagtag tctttcgcaa
     6301 aggatttcac aaaactttct acttggttgg tatcgacaat ctgctgttta atgatttctg
     6361 tttcacggac agtatgagtg tcaatggcag taaaattttt atagattcca aatccgacac
     6421 taccaatcaa taaaacccat aaaacaaagg ttaatttctt atgcgtgcca actttaatca
     6481 tcggaacttt tctttgcttg ctcggttttt tagatttctt tggtttctcc ttcttttctt
     6541 ttcgctcgat ttttataatc atttgattca ttcctttctt agttatgaat aacacgtcta
     6601 gccccaatca gatggtcttg ccaataacga ctgcttaagt cgccataacc gattgggtcg
     6661 cctgcatgat aaaagcgatt tccctctaaa tatatcgcca catgagtcac atatgtgcct
     6721 gcattatagg ttgaatgaaa gaagataagg tcgcctgctt gggcttcttc catagagatt
     6781 tcttgcgtag cgtcatactg atcttgggcg acacgaggga gagaaatgcc agctttgtca
     6841 tacacccatt gtattaagcc actacaatca aaagaggtgg taggcgacgc ccctccaaag
     6901 acatagggaa acccttcgta tttcaaggct tcgtccataa tgacttgcac ggtttcatca
     6961 tcaaattctg ttggggaagt gtcggttagg tattgtgaaa ccaactgaac ataaaactga
     7021 tttccatagt tatatcgcca accaccattc acaggtatgg ctataggatt gggatagtct
     7081 gctttttgac cacccgactt ctctttagaa aactgttcgg ctaattcata ggtatatttt
     7141 ttcccatgac tcctcacata atttaaaaag ccaccgccat aattataaga ttggataaca
     7201 gaatctatat ccactccttg ttgttctgca ctggttaata attcactgaa atatttaacc
     7261 ccttgtttga tggactcttc ggtactcaaa ctgttgggcg gtaaacccaa agattcggaa
     7321 ctttgcataa catcttctgc tgtaccgccc gattccactt gcataatggc taagatataa
     7381 gaaacatagt cttcaatccc atattcctta ccatattttt caatcaacgg acggtgtgcc
     7441 aacacttctg gtgaaacggt cacaccgcct tgtggaatag aagaatcctt gttgttttcg
     7501 ccactgtccg tatcatctgc aaagaaaatg actaggaata ataagagaaa aaagaagaga
     7561 gagaagacca cactacaaat cactgttatt tttagcttca tgtttgttgt cctcgcttat
     7621 ccgttctttt atttagcttc ctctgattga attgcttcgg tggtgtcttt tgtttttgct
     7681 cttttttcaa tggtcgctga tgattttcac gtttagtgtt ttgtgacaaa acaggcgtga
     7741 cggtttgctg acgcttgcga acatgctgct ggttattctg aacaggagat gatgggtttt
     7801 cttttcgtct tttttctttt gctttatcca gctccaaacg cttttgagca atgttctttc
     7861 gtttttgctc ctgctggttc atacgctgtt gtttgcgttg agctttggta tctgccatag
     7921 attgtttaaa gtctgttaca ttatgagtga cattctcttt tccttgatga agggcatatt
     7981 tgacatgggt tggtgtcgcc tgtatctgtt gttttgcctg ctttctttta ttgactagac
     8041 gatttttcgt gtccaataca tgggctgtct tttcacctaa tcgttgcccg ataccgcctt
     8101 gttgcttttt cacattggaa agtgctgttt ctttctttgg tgtagtccgt tcattttgac
     8161 gagtatggtt agctttattg gtttgattct gctttgttgt cattgtcttt tgcttcgttg
     8221 accctgcttt tccacgaaaa atcttactca tactgcgttg aaatcttctg gtttgtcgat
     8281 tcatcatttg acgaggtctg cgaaacactt gtctgcccat gttttgagag tcgttgcttt
     8341 gtaaagtgaa taggctcatt aaatcgccca gtttaaaata aatccctgca aaggtcacaa
     8401 tttgtaaaaa cataatcatg aaaaagggag aacttgctga cagcgaataa agcatagtgg
     8461 aaatactaaa ggcaacggta ataatcaagg taataccagc ccttgtcata atcacattaa
     8521 ataacttggt aatggcatgc ctgcccatgc catcaaaact cggcaacata cttaatagga
     8581 aactgattgg caaaaacata gcgaaaataa taaatagcac ttgcgaaaaa atcatgattc
     8641 ctgttagaag gaaaacaaaa atcgagatac caatattgaa tacaaacaaa aagaagactg
     8701 ttcctaatct ggaaatcgtc ttcggtaggg ttaaataggt attgtcatga tcttcaattt
     8761 cttcaatcac tacattttct ctatcttcac cattgtttgt atctggactg gtagacaata
     8821 aactttccac acgttcttcc cctaacgctt ctacatcagt ggagccatat tgaagcaata
     8881 gccatggttg ctttacttga acagagaata agctgtcacg tatcatgtcc acactgtctt
     8941 tcccttgact atccgtgtta ggaagaacaa tttttgtccc aatattcaga ctggcggtac
     9001 tgacatcttt ggaaaaatca ttgattttcg caatataatc gggtgcatac gcaataaagg
     9061 aagccgatag aataaacacc acgacaaagt ttaaaacagc tcgtatcact ttcgtcgttt
     9121 ctcgcttaac caatcctgta taggcaacat agattccaac aatgagaata aaaagaagga
     9181 ggaaaccgac ataaaaccct gaactggaaa agccattcgg tgtaatgcct gcaatcgtct
     9241 gcatgttttc gccaattgct tgggatgttt gactaatgaa atctaaggca taggcttctt
     9301 tcaccaaata acctgtggcg ttcgatagat aaagagaaat tgtccaaata aaattagtaa
     9361 tggcatacaa tccatacatc accgattgtc ctatgccatc actccaattc catggaagcc
     9421 agccccagct attgtccaca taataatcaa gctgatattg attcaaacca tacttggagt
     9481 attcattgct tgtatcaatg gtttcatcaa ctaagcctgt cgcttgtact gcattgccca
     9541 gcgtgcctaa taaaagtaaa ataacgccca cacaaaggag cgttatccaa ctatagtgaa
     9601 gaaatttctt ttgtttttgt gtcattcttc cacctcattt cgcacaggcg gtctggtatc
     9661 aaaggcatga agcaactctt caaaaatcgg gtgaaactgc ataaccccta cacgaccata
     9721 gatgtctgaa actaaacatt gtccattttc taattcacgt aagcgtttct gattgttttc
     9781 atcttccttg tccacaccaa aaaattctaa ggtctttttg atttccacta ggtcagtgga
     9841 acgaaaggca aatttcaagc caatattatt cttcatcttt tcatctagta aatcgtccgc
     9901 gttttgagta acgaaataga cccctgcatt catggcacgt cctgctcgaa ccaatcgcat
     9961 agatagagct tttccttgtg ccacttgtaa aaaactccaa gcctcatcaa ggtcaacaat
    10021 tttaaaaatg gaacggtcag agtggataaa gtctaaagca aaggtactaa tgacaatcag
    10081 cattgccaca cttaataatt ccatggtagt gtattcttca aaagagctat tggaatcggg
    10141 taagaccaaa tctgccactt gaataatatt gagttgtttt tccaagctaa tggattgggt
    10201 gacactgcca tcactgaata acagatgggc aaaatcataa tcgacaaagc tttcgatatg
    10261 gtcagcaata ctattgctga tgggcgtatc ttcttttcgc aattcatcaa tcaccaataa
    10321 caaccctcgt tgtttgcttt gagttacact ccgaatagct ttacgcagga caggaaactt
    10381 ctcgctatct cggctggaaa tacctgttaa gaacgtgaga atatcaatgg caaggctttc
    10441 tgaatctttc gggagtttta aaatcacata agggtcaagt aaccctttat tgtcgttctc
    10501 actcgtgaga ttaacaatat tgatttcatg ggcaatatca ggtaaggttt ctttccattc
    10561 accacgttca gcttttgggt caacaatggt tgcctgtccg ccaaagagta ctgaataata
    10621 gaccagcata ttgttagaga atgattttcc gccacctaat gaaccaataa aagcggacgc
    10681 taatgcattt gttacagagc ctttaattcc ttgacttgca aggctaggtt ggagataaat
    10741 atttctgcct gtatcgagat tgtaaccgac gtaaatacca gaattttcac caatcatttg
    10801 tgtagcaccg aaaccaagac cagccaaaaa atccgacgtg acatattgaa tatagtcatt
    10861 gatatatcgc ttgcttgagg gaataaattc attatgcaag cctagcatat cgccaaaagg
    10921 tcgtactaat ttgatagaca gatcatcata aaaatctttt acttcatcac aacgttgttt
    10981 cagctcgtcc aaattctttg ctgatacccg taccacatag cttagtttgt acatagattc
    11041 tttggtttgg tctaaattac tttctaattc atcgacagat tctaaagcgt ccaccacatt
    11101 agtactggtt tcgttgtcgc tttcccatgc gtggctatct aaatctttca gttctttctt
    11161 tttattgcga acggtagata aggctttttt attggcgaca atttcaacat tcatactggt
    11221 atcaacgggg aaagtaaact gctgttgctg gtaataaaaa atttcactat tagggaaatc
    11281 caattcacca acaatactat taattgtaaa atacgccaca taagtagtgg tatcttcctg
    11341 ttcaatccgt aaatagcgtt gattttcttc gattaaacag cgagtgggtt tgagaatatc
    11401 gtatttcttt accaacactt ttttatcttc gtatcgtttc ggtaaatggt agtcgtagtc
    11461 atcaaaaggg acacctgttt gcccgtaaag atgttcaata atataaccaa aatcatcttt
    11521 ctccaattta cgaatctgaa agcgtctgga taatttactt tctaacaact gttccatctt
    11581 agaaaagcgt tcgatttccg ccataggcat agaaacaaaa tcgcccatta atttgtgatt
    11641 cacaccatga aagaattccg caaatgcttt ttgaatttct ttcccaacat tttttacgga
    11701 tacttcctgt tcattgaggt agagtttaaa accaataaaa aagcgatagt ctacttgatt
    11761 gtcaccaatc atagaaatta acgcttcggt ctgttcatct attttttcgt aagctatctc
    11821 ttttaatcgt ccagtgattt cttttttcga ttgttcctgt gtggctcgaa tacttgattc
    11881 ggttgctagt tgcagagcat gaatcttgcc atcacgattt tgggcaatca attgacagaa
    11941 attatcatga acttgaattt tttcatcgga acttaaaaaa ctgtaattat agggaattaa
    12001 ttcataataa gcgaatactt cgccatcatg attaaatacg agattatctt ccatatattt
    12061 aatcggatat gccatctagt tgttcactcc ttacaatcgt aatcgattca ttcattgttt
    12121 ctttttttaa tcgtatttgt ttgccagcgt aggttacttt agggcgtaga gcataggtaa
    12181 tgatagactt tagaaatcca tagggtttct ttccgtcaaa ggtcttttgg cacatgaacc
    12241 cagtgagaac aatgggaatc cctaagtatt tgagtagtgc tccgtcaatc agagaaagtg
    12301 gtggtaaact gccaaagagt atgacgacaa acaaagaaac gataaaccat gtcatttgcg
    12361 taaaggtaat tgggaaaggg agttgtaaat cattaatcgc ataaatcact ttttctactg
    12421 accaaatact ggtgtaactt cttatttttt tcatgtcttc acgtcctttc cgtaaaaata
    12481 gaaaaagaac aatcaaaata ggtgattgct ctttcaatgc tttaactatt caattttatt
    12541 ttttgtccat tttgcaataa taccttcatc agtttcttca tcatctggat tcattgaata
    12601 tctgaaactt aaattggtat ctgaccctaa tcgttcatat aagctgggtc tatacaatct
    12661 cgttttattg tcattaaaag gacttcgttt tcccagctca ccatttccct ttaaaggatt
    12721 tcttctaact gaaaaacaat atttttttct gggatcttct gaatcacttt gacttaatga
    12781 gaaacacatg gcttcttttt ggtcttcatt tttcttctca ctaacttctg tcggaataaa
    12841 tcgagcaaga gtttggctaa attgaaataa aacatctccc aagttactac tgtattcgat
    12901 accaaaatat tctcttattg ttttcgtgtc tgtatataat tttaccgaat tagcatagac
    12961 agctagcata tctgaaaaat cgttttcctt taaaaattct agttttaaca gagcatattc
    13021 tggaactttt tcctcttttt caaatagctt aacaagtaca atataatttt gctgtttgta
    13081 acgaaagtaa aaggaatcta tcacccagcc tttatctttc atgtctagtt ttaatagttt
    13141 aagattttca aatacattta ccataattta tacctctccg tgctatttat taatttaatt
    13201 ataacacgga ttgttaaact tactgacaat attcaaatac cccatgagag gtaactaggt
    13261 aattaccctc tatttccata tcacgtccca aagattggta gtcaatataa ttttgtaaat
    13321 tggaagggac ttcaccaagc tgtcctgttt cttccacgta ataacgggcg acatcttcca
    13381 tcgaatcaca atcactataa caatgaatat cctctttgtt ttccagtaat tcttccaagc
    13441 tactaaacca catgccttga atttctttga gttcattata aataggtgta ccttcgattt
    13501 cttgaatggc ttcacataga cggttgattt cactaatcgg cgtgtattcg tccacatcaa
    13561 aaggaagctc aaaatcatgt attgcatatt cttcgtagtc ctcatttaaa ccgattcttt
    13621 ctgccatttc ttcctcatca attggtggag taaaccatgc tccgactaat tcaccttcat
    13681 tataccgtcc taaatttgcc acatagactt tcatttccaa ttcttatcac atccttttta
    13741 taaaaatagt cataaaaaaa gtagcaatca gttaatgacg ctacttatca aggtatccgc
    13801 taatataatc tgtaactgta cttgaaatac ctattattag ttttgcttct ctctttccaa
    13861 taatatttgt attagggtgt gcaagtgaat gcctatttct tgcttcatta agagagctaa
    13921 tcattccatt tgatgacctt atcgttgtct tcactatatc agcaagttca gtaggttgaa
    13981 tttctttttc aattaattgt tgtatcctac tatataattt agatagattt tcgtctctag
    14041 gaactgtaat ttcatatttt tttagtattt caattaaata tccatgaaaa gcagtgtgaa
    14101 ctctgtcaaa ggcacgttga tattcaccat tactcataaa agactcagcg tcttcaatag
    14161 cttgtgaaac cacatcacta attttcaact gactaaagtt aattacttcg taattctctg
    14221 gaagtggtac taaaatagta tccactaata cattatcacc ttgtttatca tatatggagc
    14281 ttgctgtctg ggtaatcaaa tctttatgac tttcaaaaaa gtctaaatca tcaaaaggaa
    14341 ctcttatttt aagatatgtg caaagcgtat tccagccctt tttatatcct ttataataag
    14401 taaaatcgtc agtctcatct aaagttacct ctgatatttt cattttagaa acaatctcgt
    14461 tacctccttc ataataattt gaagagctct ttctttctaa tgttttaatt aaaagagatt
    14521 taaattgatc atttgtaaac atattcataa atcctttcat aacaaaatat atacttccta
    14581 ttttatcatg aaaaatatct gttaagcacc aatgattcgg ttaaataagt ttaataacac
    14641 atctttcacg ccagctgcat tgaacacaag tccgacggcg attagagcaa taatcaagaa
    14701 gccaatgagt tttgaaaatt ctcgtttaaa acctaaaaac agtccaatga caacaatcgc
    14761 taacaatacc aatgactgtg cattatccaa gaaccagttg tacaaattct gcccaaaatt
    14821 catgtgattg tcctcctttc cttaagatat ttttctcgat aatagatata gctttttaca
    14881 cgccttaata attgaaaagg ggttaaatgc tctccataga cggttactcg attggtataa
    14941 ttattataaa atctcacctt gtaaaaatca taggtataga tggtatcacc agcttgccgt
    15001 tttccctcaa taatgagaga cgtatatttc cctgtatttt gtatatacgc ttgaaactca
    15061 atgccacact gaaaccctaa cttctttaat tcttttgtta aaaggctcat gtaatcaaat
    15121 cctccatggc tagatgttgt tgttcaatta atttctcatg tttttctgtc agtttggctt
    15181 cctgaatcat ggtggagata atggttgtac cgttgagaac atctaaaatg cttgcgattt
    15241 ttaatgtagg ggcgacttga tgatgtaacc agttcaatgt tcgttcaaaa gaataaggct
    15301 ctggctgtgt ggttaggcgt aatgctccac gatttttacc gataaaccat tcccaacgtt
    15361 cattagtttt ccaatcactc cgacgtttag tgtcgtcttt atctgcaaag cggatatagc
    15421 gattgataat ttcaaaggct gttttttccg cctgttgatg tgccaataaa tctttcatgg
    15481 cttgggtggc tcggtcattt tttaaccgaa tttcaaaccg attctttact tctgcgtctt
    15541 ctaagggaac atcatttttc acgtattgct cataatcttt ctcatatata cagaaataga
    15601 cttcactttt taatgagcca atataaagtg tgtttcccat gtatggtttt tcatcacggt
    15661 gaaccaattc accactgcga taatttttaa agcttcgaaa gacggaaatg cattcttcct
    15721 gtttacattt ctttgccaaa tctggaatat tcaaaatccc tgttttatcg ttgatagcta
    15781 aatccaagcg tttgaaaata cctttatgtt ccatgcaatc ttgaaagaaa tcataccagc
    15841 tccgatgttg agccaataga aagttttcaa attgccgaca gccttttcct ttcagttcta
    15901 ataaaacacc tttacttaca tcatgagaga ctaggacaac aatatcaccg aaacgatagt
    15961 gttcgggata agaataatag ccaaagtctt cgtgtagcat ataatccata ttcaaacgta
    16021 aaacctcacc gataatttga gccacatcat tggtagggaa tcgaatccgt acatagtcga
    16081 ataacatctc caatgggtta tcgggattga agcgttccaa agcgttcagt aaatcttctt
    16141 tcacctgttg agtagggact gccttgcctg tttcaatatc cgataggtaa ggtctggtaa
    16201 tatgagaagc aaccgccagt ttattttgag aaacgccata agccagtcgc ttttctttca
    16261 attccttaat ccaaattttt tcgttttcca ttcgcaaacc tccaatctaa aaaagatgta
    16321 aactatgacc cttgagttta cacctttcat attttccgca atcctttgta ccatcaacat
    16381 tggttgatga gaattgcctg ttttcagttg tatttgtgcc cccctgttag atagaggggg
    16441 ctaaaggatt ggcgtggcta acgccacacc aatcaaagca agtctgaacc tgtacctttc
    16501 acttcgtgcg gtacaggctc agattgcttt ttttgaatga cttttcctat ttctcctaag
    16561 aaatcgtacc tttttggtac aaggggtgta taaaactcac taatcacact ttttcctgta
    16621 tccacgtaac ctcttccctt gatgggtttt aagaaaaatt gtttttgtgt atcgcttccg
    16681 aacatcatac caaagcccat ttcagacata cgacccaaag ccacacggaa gttaaagttg
    16741 tcccgaatcc catcactaaa atattttgcg tctggacgtt gacaggcaag aattaaaaag
    16801 aagcctgctt gtcgtcctaa cattacaatt tgtttgagtt tatgaataac ggtggtactt
    16861 tcacgaccta gcatatccat cattgccaca tattcatcaa aaataagaaa attggcagac
    16921 aaaccaagat aagcataatt ttctcctgtt ttatagttag ggtgttgttt catggtttca
    16981 ctgcgatcaa tcatatcttc gtaaaattga ttgatacatg ccaacatatc ttctttacgg
    17041 taatagacat tattcatgac cgcccctaag tctgccaaat cagcattttt agggtcaaga
    17101 atataaagct ttgcatttgt atgcaggaga gattcaatca atgtgagaat aaaataggat
    17161 tttccactac cagtgccccc agcaattaac atgtgaggga gagaatcgaa ctcccaatag
    17221 accgattcca tcaatttgac acgtccatct tgagctacca cgtcttcaat gccaattcga
    17281 ttgccaatag tatcataaag taggacatac tctacataag aatctttcaa cgttttttcc
    17341 actaattcac aatacaatcc tgtttctagt ttgttttcta ggtgcaaaag ctgatcttga
    17401 tatttcccta aggtgatttc tacatgaata tggattagtc catctttcat tcgataatat
    17461 attttaggaa aatggcggat tcgttgtttg gttttcttgc tgggtaaatc tttaaagaat
    17521 ccctctgatt gagtttcttc ggcttcatac caatgatttt ctaaaatcat acgagctaac
    17581 ttttgacggt gctctaactg ttttatagaa tctatacgat agtgataata aagccagctg
    17641 ataagtccgc atacaaaaag agcagtgaga atactgctcc aaaagtatgg cgtatgaagt
    17701 tgatagaaaa atgtatctaa ctgaactgct tgccagttca ccgattgtaa cgctctccaa
    17761 tgaaaaggga gtaggacgac tagaaaaact accagcaatg aaattagtac aaaatgatag
    17821 accaagtgct tatcgttggc tttgattcgc tctcctctgt catgaagcaa tggcataaaa
    17881 atcctccttt ccacctatca aaataattgt gtattgtaga tacgagcctg tttatcaaaa
    17941 gttggttgag aaatggcttc atatttctga taagcgattc gcttttcaaa gcgtaaattt
    18001 ttttctttcg gataataatg acgtcgatag agagtgtgtc ctaacagttt tttcgcttct
    18061 tttgtcaacg gatagagata gcgaaacatg cgtccattga ttttctcaat ccctttgtat
    18121 tcgcaaaatg cctgggaaag ccaatgcttt ttctctacct gttcaaagcg agcattttcc
    18181 tctaataaaa ggcgagcact tcgagggtgt atcttttccc atgactgctt atcacgatag
    18241 acacttgtct tgaaataccc gcagtaaaag aagttggacg cttgatagac atagccacat
    18301 tttccctcaa ttccatctgc cagtgtatat aaaaaatgac agtccgtatg ttctttcaac
    18361 cacttaatta gagctgaaag agcttgactg ccaaaataat tggtttgatt catttctgga
    18421 agaaaacaca tctttccaat ttctaaataa tctgtggttt gaagggaact gtcgggaaag
    18481 agtttgcgta tggtttgtag gggttgcgta ccccagccta attccacaac gcctaataat
    18541 ttttcttctg aaaaaatccc tagaaagtat ttacacaatc tagggagtat ctttgaataa
    18601 tggtattgcc gaataaaaga aatggcttca tccttcggaa tttctttcaa aatcaatggt
    18661 gtgtttgtca taagcaacac ctatttttca ctttcggacg cttctttttt atatttctga
    18721 tgaggttttg tctgtccttt taatacaaga tcgtctgctt tcacgtacca atcaacgtct
    18781 gcccctcgaa aagtagccgt cgctacggta tctgccacag gattgattaa ttccacttct
    18841 gcgttgtagt caaattcttt caaaggaaca gaagcgggga tactgacttg tatcatgcgt
    18901 ccttgtcctt ttgattttaa gtcataggta cgttctttaa tttcttcgga aaccgtacca
    18961 tcttcattct ggatacgaac ctcacgacgc aaggcggaaa atttcaatgt gccaaatgtc
    19021 tggtcttttt caatcacaat accttctgct aatctcatat tttttcacct ttcctttctt
    19081 atgctttcac catatcgtct gcgtgcataa tgtaattggt aaatcctcga ttgccaattt
    19141 tataaccttc tgcggtaata tgtgggttga tgagtttgac tttttcgtcg gattcaaaat
    19201 gtttctcgcc agcttcggag ggaagaatga caacaatatc atctgctcgt tgaatcgttg
    19261 agtataaatt gtaactacga gagagaatgg ttggtctgcc atttattcga cgttgttcaa
    19321 tgttcccttc accagcatat tctaattgtc caaatgtttt ttccatattg ggaatgacat
    19381 atttaagttc catattttta cctatccttt ctgtttttat gtttgaatga atcttatttg
    19441 tgtgatggtt ttaaaggggg gagagcgacc ttttgattct tgatttttta ttttcataag
    19501 ttcacttcct ttcaaaattg ggtaaaaaaa tagacgctcc gtactgagcg tccgattcct
    19561 actatattta aaacaacgac tacctaggta ttttaaat
//
LOCUS       DARBET010000243        16936 bp    DNA     linear   BCT 07-NOV-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4027
            SAMEA114569841-rid20112183.guided.056, whole genome shotgun
            sequence.
ACCESSION   DARBET010000243 DARBET010000000
VERSION     DARBET010000243.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA114569841
            Sequence Read Archive: ERR12203666
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 16936)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16936)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001972618.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001972618.1
            Genome Coverage       :: 132x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/07/2023 13:18:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,041
            CDSs (total)                      :: 2,975
            Genes (coding)                    :: 2,908
            CDSs (with protein)               :: 2,908
            Genes (RNA)                       :: 66
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 67
            CDSs (without protein)            :: 67
            Pseudo Genes (ambiguous residues) :: 0 of 67
            Pseudo Genes (frameshifted)       :: 26 of 67
            Pseudo Genes (incomplete)         :: 28 of 67
            Pseudo Genes (internal stop)      :: 20 of 67
            Pseudo Genes (multiple problems)  :: 6 of 67
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16936
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA114569841-rid20112183.guided.056"
                     /isolate="VRE4027"
                     /db_xref="taxon:1352"
                     /country="Denmark"
     gene            complement(134..577)
                     /locus_tag="SCJ86_002998"
     CDS             complement(134..577)
                     /locus_tag="SCJ86_002998"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF305 domain-containing protein"
                     /protein_id="HEG1170464.1"
                     /translation="MKKYWKFLLMIGVSTVVMFGMMYLNVYALDHIFFSRTRVFMALM
                     MGAVMAVIMLLFMWKMYENKKINVMILAASLLVFAGSLFMVRSQTTVNDTAWMKAMIP
                     HHSIAILASKRADLKDPEVKKLAEEIIQAQEEEIDKMKKMIKEME"
     gene            complement(795..2135)
                     /locus_tag="SCJ86_002999"
     CDS             complement(795..2135)
                     /locus_tag="SCJ86_002999"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HEG1170465.1"
                     /translation="MEDKKLKKQLSSRHITMLALGGAIGAGLFKGSGEAIGIAGPSVL
                     IAFLIGGLILYIVMKGLGKIVLSGGDTHHGLSGLIRPYLGAHSADFTDWVYWSMWIIN
                     IIAEAVAAASFLQLWFPHIPAWVFVFMLAVLTTIINLYSVRLFAETEYWLAFAKISVI
                     ILLIIFATYLVGQQMLGTGVFPTLQQLTDHGGFTPHGMKGIISSLLVVIYSYGGSELI
                     AITVSEADDPKRAIPKAIKGVMGRIISFYIIPLFLLLILFPWNTLAGTNVSPFVMVFE
                     KMNIPFAADIVNFVIVLALFSSINSGVYASSRILFFRLKDRKGSSRKLAVLNKHQVPQ
                     RAVLFCTSVLYLGVALSYFVGDKLFGYLAGSLSYTVLLIWIIISAASFVLALRKGTLW
                     DKGISFFALAVLGLIFLGILFTNSIGVTVLTGLLYLFIYFSYQKKNDAFVLTNE"
     gene            2404..3300
                     /locus_tag="SCJ86_003000"
     CDS             2404..3300
                     /locus_tag="SCJ86_003000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323722.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oligosaccharide biosynthesis protein Alg14"
                     /protein_id="HEG1170466.1"
                     /translation="MNKNDVLLVAKNKSLYFLPSIRSLDVWSGKDLLQSEFIKSARQQ
                     LANYQLIVFLDYGFDMEMARRVRPFTNAKIVLFFWNHFKAEHEAMLKQAQDEPAIDEI
                     YHFDFLEAKELGLKHNSSFYSKHMNFSSSEPKTDLFFGATNNGRKERAESYKQEFIKR
                     DVTTNYFILPHRGNEQPGYLTYEEYLKKTGESRGILELLREGQQGVTLRTFESMYFQK
                     KLVTDNQAIAHYHFYRPENIFLLRERDLDELPEFLHTPFKPILSELLEFYDAENWAKR
                     FITNDSTIYEQYEYDLSSVENK"
     gene            complement(3450..4391)
                     /locus_tag="SCJ86_003001"
     CDS             complement(3450..4391)
                     /locus_tag="SCJ86_003001"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-dehydropantoate 2-reductase"
                     /protein_id="HEG1170467.1"
                     /translation="MKIAIAGAGAMGSRIGLMLHQSGNEVLLIDRWPAHIEAIRTNGL
                     IADFNGKEVVAKLPIYSPEEIIESNEHVDLIVALTKANQLDDMFCSIQSIITDNTYVL
                     CLLNGLGHEDVLEKYVPKKNILFGITMWTAGLAGPGKVTLLGDGEIELENLEPEGEAF
                     TKKVVEVFQEANLNPIYSHNVRYSIWRKACVNGTLNGLCTILDCNIAELGAQKAAESM
                     VRTIVSEFASIAAKEGIILDQEEVYQHIASTYDPDNIGLHYPSMYQDLIKNHRLTEID
                     YINGAIWRKGQKYDIATPYCAFLTQLVHAKEGILGAE"
     gene            4517..5638
                     /locus_tag="SCJ86_003002"
     CDS             4517..5638
                     /locus_tag="SCJ86_003002"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerate kinase"
                     /protein_id="HEG1170468.1"
                     /translation="MDILAAIDSFKGSASSFELNQAVLNGWEDRKGRKINVPIADGGE
                     NTAEAIYYALRGTWRTIDGMDLLFRQRKISYLLTTWQGKNTAVIESAQALGLDLLDKP
                     TDQTIRLASSYALGELIKDALLQEVEQIIVTLGGSGCSDGGLGLLQSLGASLEGYTEG
                     NPLLSTKKLHFDRRNVLVSKVKLIIAADVTNPYTGQQGAAWQFGRQKGGIDTTLAFLD
                     KQAGKIAQQIKDFWEVDLDDIPGSGAAGGIGGALFLLGAKMVSGFDLVSQITGLEKKI
                     EQADVIITGEGRIDQQTIHGKVPYGVAVLAKKYRKPIIAICGSRDQELEGLSDFLPIV
                     LSIHLGPIGLEEAMEHRQTLEKTKILGQTLSRFFTILPK"
     gene            5896..7398
                     /gene="eat(A)"
                     /locus_tag="SCJ86_003003"
     CDS             5896..7398
                     /gene="eat(A)"
                     /locus_tag="SCJ86_003003"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F type ribosomal protection protein Eat(A)"
                     /protein_id="HEG1170469.1"
                     /translation="MSKIEIKNLTFGYDSQGTLLFEQANLNFDTQWKLGLIGRNGRGK
                     TTLLNILQNKLPYQGQVIHQQEFAYFPQQTKDKERLTYYVLNDITDFEIWEIERELQL
                     MQTDPEILWREFSTLSGGEKTKVLLALLFVDDTHFPLIDEPTNHLDISGRKQVAAYLK
                     KKKQGFIVVSHDRGFIDEVVDHVLAIEKSQLELYQGNFSIYEEQKKLRDEFEMAQNEK
                     LKKEVSRLKKTAAEKAEWSRSREGDKTKKQVGFIDTESRRVNKGAVGADAARTMKRSK
                     AIVNRMETQISEKEKLLKDIEYIDSLTMNSQASHHKRLLSVEDLQLGYENLLFEPIHF
                     TIEPHQRVAISGPNGAGKSSIIHYLLGAFNGKVIGEKYQPKHLSISYASQNYEDNRGT
                     LAEFAEKNQVDYQAFLNNLRKLGMERDVFHNKIEQMSMGQRKKVELAKSLSQPAELYT
                     WDEPLNYLDVFNQEQLEQLILNVKPAMLLVEHDQTFLDKVSTEIISLERI"
     gene            7426..7992
                     /locus_tag="SCJ86_003004"
     CDS             7426..7992
                     /locus_tag="SCJ86_003004"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine phosphatase family protein"
                     /protein_id="HEG1170470.1"
                     /translation="MVKGNFANEGENHMLYVTRHGETTWNAQGLVCGRADVPLTEKGQ
                     MQAQKLAEKVVDLPVPITKIIHSPLQRARDTAQAVADRLSLPLTVDERLVEMDFGDYD
                     GLPSKDENFQKARLAFAVRFPNGESVLDVYARIVPLLKECIEDEENVYLLVCHNALIR
                     VINAYFHPMPNEGFFTFMVDNTELVSYE"
     gene            8203..9264
                     /locus_tag="SCJ86_003005"
     CDS             8203..9264
                     /locus_tag="SCJ86_003005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002368660.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF871 domain-containing protein"
                     /protein_id="HEG1170471.1"
                     /translation="MFGFSIFMNDSLSEEKKQYIRQMASNGFVGIFTSMHIPEDDVSA
                     YQKRLIELGSEAKKNKLELMVDISGEALERAGFSLEDLTPLKAIGVTGLRMDYHISNQ
                     QIADWSHQLKISLNASTITPKDIDELKEAEADFSQIEAWHNYYPRPETGLDKEWYQKK
                     NQWLKIQGLLVQGFVPGDTELRGPLYQGLPTLEEHRGIHPLAGALDLLASNTDIVYIG
                     DAGLSENVQEQFASFQKEQTVLLHTEPVDEEFYEYILGKHTNRQDDARDVIRSVDARF
                     REIPPIPARNTATRMKGSITLDNEKYLRYMGEIQLTKYDLPADEKVNVVAKVIKEELP
                     LINQIKAGMNYQFIRKEGR"
     gene            9267..10163
                     /gene="murQ"
                     /locus_tag="SCJ86_003006"
     CDS             9267..10163
                     /gene="murQ"
                     /locus_tag="SCJ86_003006"
                     /EC_number="4.2.1.126"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002368658.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramic acid 6-phosphate etherase"
                     /protein_id="HEG1170472.1"
                     /translation="MSKINLENLTTERRNQETFGLDEMSISQAVQLMNKEDQKVAAAV
                     EKELTSIEPVIANTIESFKKGGRLIYMGAGTSGRLGVLDAAECVPTFGVEPEMVIGLI
                     AGGEEAMTVAVEGAEDSYELGQEDLIKLNLTKNDIVIGIAASGRTPYVIGGLDYAKSI
                     GAVTGTISCNKQAEISKHADFPIEVDAGPEFLTGSTRLKSGTAQKLILNMISTISMIG
                     IGKVFNNLMVDVKPTNEKLIERSKRIIMQATEADYETAEKYFEEADQNVKLAIVMILT
                     NSDKEEAQAKLAEANGFVKGTL"
     gene            10177..12159
                     /locus_tag="SCJ86_003007"
     CDS             10177..12159
                     /locus_tag="SCJ86_003007"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334912.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucose transporter subunit IIA"
                     /protein_id="HEG1170473.1"
                     /translation="MAKNELTLEELAKQIYKGAGGMGNVESIVHCMTRVRMKIINDDL
                     VNVSELKAIPGVLGVVEDEQLQVIIGPGKVNKVAQQMVDLAGVKLGEKLSSVSTATAN
                     SSASGKDRVNERAQEMKAAQKSKQKPSKIKSVLKDISNIFVPMIPAFVGTGIVAGIAA
                     VLANLVTAGTLDAATWQQYIDVMNILKNGMFSYLVIYTGINAAQVFGATPTLGGVIGA
                     VVMLTGMNPEAPLKNLFTGEALAAGQGGILGVIFAVWLLSIVEKKLHKVVPDAVDIIV
                     TPTLSLIAIGLIEIFFIMPLAGFISDGMVGGINWVLGVGGAFAGFVLGTLFLPMVMFG
                     LHQILTPIHVQMIDETGRTLLLPILAMAGAGQVGAALALWIRCKKDKELTEMIKGALP
                     VGILGIGEPLIYGVTLPLGRPFITACIGGGIGGAVIGMIGNVGAIAIGPSGAALIPLI
                     SDGKWLGYILGLLAAYAGGFVATFFFGIPKDRLEKEELATETATVNEVPEKVVPQTSA
                     ESVTILSSVADGTVVSLDKASDPVFAQKMMGEGYFVEPENGHIYSPVTGVVSSVFPTK
                     HAIGITTPNGLEILLHMGINTVDLGGTPFDVKVTEGQQVTPDTLVAEADLEAIKAAGK
                     ETSMIVLVTNMDRVKNFVLEKTGKAKAKAPVMDVKA"
     gene            complement(12224..12670)
                     /gene="nrdI"
                     /locus_tag="SCJ86_003008"
     CDS             complement(12224..12670)
                     /gene="nrdI"
                     /locus_tag="SCJ86_003008"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299965.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class Ib ribonucleoside-diphosphate reductase
                     assembly flavoprotein NrdI"
                     /protein_id="HEG1170474.1"
                     /translation="MNILYISISGNTRAFAKHLAEYAEKMHAEDPVNPEVTLKEIHEN
                     SDFTKETEPFFTFVPTYLDGGNGLDNGDTEILTETMREYLEHEDNHKLCLGVVGSGNK
                     NFNNQYCLTAKQYAQTFGFPFLADYELRGTPSDVTRIYQILAENNK"
     gene            12881..14305
                     /locus_tag="SCJ86_003009"
     CDS             12881..14305
                     /locus_tag="SCJ86_003009"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853071.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HEG1170475.1"
                     /translation="MDTEQQKKGIGLIPLAALVIGSAIGGGVFGIMTDISGSAGGPAI
                     FSWVLVGTAMLMLSLSINNINKKRPDLKGGIFSYAEEGFGRFAGFISGWGYWLTCWLG
                     NVAFATLLMSALGYFFPVFEGGQNLASIVMSTVFLWGYAWLVNRGVENASFVNAIVTF
                     CKLVPLFLFIVVAISTFSIQQFIDNFSSDIILNANGQPIPFSSQVLSCIMVMLWVFVG
                     IEGASVVGSRAKKQSDVGKATILGIFGLIIIYVLVSMLPYGTMTMNQLQTIKAQPAMG
                     YVFEMMVGKWGAVVINGGLIISLVGVWLSWTILPIETMGNMAEDGLLPKAWGKLNKNG
                     APTYAIVLTTLCTNVFLLSLLFTDSAYNFAYTLGTAAIFFTWLFLGLYQMKLSYQRKE
                     WVQFLVGLLASAFQVWAMLVVAFNEVMLVLVLFLPGIYFYLKARKEQNEPVQHIHLDK
                     ILLIGVTMVGVIAMVLFATGMLHV"
     gene            complement(14486..15259)
                     /locus_tag="SCJ86_003010"
     CDS             complement(14486..15259)
                     /locus_tag="SCJ86_003010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373993.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HEG1170476.1"
                     /translation="MTLLVKQELFKLIKKKSTAVLSVLLVVLLIGTALLAKKYTTIID
                     PVEMTAQLFSATSWIVFIMIAAASTIISMEAQYGTLKNLLYRKYSRGEILVSKWITLV
                     IYSVYLYLLAIIVTVLMKLILFPSISFTEKVSTGQTLIQSLFTYTLGSYIGLWLILSL
                     VLMIACFINSSGASISAGIVFYFASSIISGILIALIQKWEWIKWNPISMLNLQNQIGN
                     EEIMKQLTHLSTNQMLFGNLAYIVLFLALGYLVFKRKNV"
     gene            complement(15256..16161)
                     /locus_tag="SCJ86_003011"
     CDS             complement(15256..16161)
                     /locus_tag="SCJ86_003011"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002316458.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HEG1170477.1"
                     /translation="MKSLSVQELSKNFGHKQALSDVSFSLSPGKIVGLVGPNGAGKTT
                     IMKAILGLIHYSDGTICIGENEISPSSHKGLEQVGALIEYPGIYPFLTGYDHLKLFSE
                     TNDVSAIDTIVNQLKMEKYIHKKAKSYSLGMKQKLGIALALLNHPSIVILDEPMNGLD
                     PQATRDVRKIITDLATQGTSFLISSHLLSELEKMVDELILINQGKIIKQCTMAELNQT
                     AQDFIVISTTNDLAARTILTEAGYTLEDPDKIKLLKENEELLGTVIQLLSAQNIHVTD
                     VQHLTNDLESSLLEILDSVKGEEEK"
     gene            complement(16315..16635)
                     /locus_tag="SCJ86_003012"
     CDS             complement(16315..16635)
                     /locus_tag="SCJ86_003012"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005877273.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux SMR transporter"
                     /protein_id="HEG1170478.1"
                     /translation="MAWMELIIAGILEVFWSTMMKWSDGFSKINYSFYTVIGMIASFY
                     FLSKAIKSLPMSLSYPIWTGIGAVGSVIIGVVLFHDKLNLSTWFFVGMLLISIIGIKI
                     TSGH"
BASE COUNT         5464 a         3092 c         3476 g         4904 t
ORIGIN      
        1 ctgttcttat tacttgatgc agggcagctt tttcacaacc ccttattcac ggcgttccag
       61 aagtagcttc ttcttatttc ttgaagctga taacttctgg aacatcctct ttttttattt
      121 ggacggataa tggtcattcc atttctttga tcattttttt cattttgtcg atctcttctt
      181 cttgtgcctg aatgatttct tccgctaatt tttttacttc aggatctttt aaatcagctc
      241 gtttacttgc caggatagcg atagaatgat gaggaatcat ggccttcatc caagctgtgt
      301 cgttgacagt agtttggctt cgtaccataa acaaggaacc ggcaaaaaca agaagactag
      361 cggctaagat cataacattt atctttttgt tttcatacat tttccacatg aacaacagca
      421 tgatgactgc catgactgca cccatcatca acgccatgaa tactcgtgta cgactgaaga
      481 agatatgatc gagcgcatag acattcaagt acatcatacc aaacataact accgttgaaa
      541 caccgatcat caacaaaaac ttccaatact ttttcattta aaacaaactc ctttttttca
      601 ttcctttctt ctatcatgtc atgagcatgg agaacaatca attattttgc acagagggtt
      661 tttgaacacc gtttaccaag aggtcatgag actgtttatt cggaaaaagg agttgcaaca
      721 agacttttgt cacaactcct ttcttcaata gctccatcta ttccacataa ataaaataaa
      781 attttctatc gatttcattc attcgtcaat acgaacgcat cattcttttt ttgataactg
      841 aaataaatga acaggtaaag caggccagtc aatacagtta ccccaatgga gttcgtgaac
      901 aggataccaa ggaaaatcaa tcctaagaca gctaatgcaa aaaagctgat tcctttatcc
      961 cataatgttc cttttcttag tgccagtaca aaggatgccg cactgataat gatccagatc
     1021 aataatactg tatacgaaag ggaacctgcc agatagccaa ataatttgtc accgacaaaa
     1081 taagaaagag ctacaccaag ataaagaaca ctggtacaaa ataaaacagc acgttgcggt
     1141 acttgatgct tgtttaaaac agctagtttt ctagaggaac cttttcgatc cttcaaacgg
     1201 aagaacaaaa ttcttgaaga tgcatacaca cccgaattga tagatgagaa caaagcaagg
     1261 acaatcacaa aattgacgat atccgctgca aatgggatat tcattttttc aaataccatc
     1321 acaaaaggac tgacattcgt gcctgctaaa gtattccaag gaaaaagaat caaaagcaaa
     1381 aacaaaggaa tgatgtaaaa ggaaatgatt cttcccatga cacctttgat tgctttagga
     1441 atcgcacgtt taggatcatc tgcttcgctt accgtgatag caatcagttc agaaccgccg
     1501 taagaataga taactacaag caaagaactg atgattccct tcataccatg aggagtaaac
     1561 ccaccatggt cagtcaattg ctgaagggta gggaaaacgc ctgtgcccaa catttgctga
     1621 ccgacaagat aagtagcaaa aatgatcagt aagataatga cactgatttt agcaaaagcc
     1681 agccaatatt ctgtttctgc gaataaccgt accgaataaa gattgatgat cgtagttaaa
     1741 acagccaaca taaaaacaaa tacccaggct ggaatgtgcg ggaaccacaa ctgcaaaaag
     1801 gaagcagctg caacagcttc agcgatgata ttgataatcc acatagacca gtagacccaa
     1861 tcagtaaaat cagcagaatg agcacctaga taagggcgta tcaaaccaga taatccgtga
     1921 tgcgtgtcgc caccgcttaa aacgattttt cccagacctt tcatcacgat atataaaatc
     1981 agcccaccaa tcaaaaaagc aatcaataca gaagggccgg cgatcccaat cgcttcgcca
     2041 cttcctttaa atagacctgc accgattgct ccacctaaag ctaacatggt gatatgtctg
     2101 gagctaagtt gttttttcaa ttttttatct tccataaatg acctctttct tttaaattca
     2161 caatcaaaat ttatattaag aataaatagc aatatttaac aatgactaat gataacaaat
     2221 tttcagacaa ttacaactat tcagaaaact tttaggcgaa ttttaataga agaatgattt
     2281 atttcatcat attttcatac aaatgttaaa ttttcataca aatataggaa acgttttact
     2341 ggctttttac tatttaaaca ggctatcata ggaaaaagtg acagagaaag tgaggcggaa
     2401 aagatgaaca aaaatgacgt actgctagtt gcaaaaaata aatcacttta ttttttgcct
     2461 tccatccgct ctttagatgt ttggtccgga aaagatttac tacagtcgga attcattaaa
     2521 tcagctagac aacagttggc aaattatcag ctgatcgttt ttttagatta tggatttgat
     2581 atggagatgg ctcgacgcgt gcgcccattt accaatgcaa aaattgtgtt atttttttgg
     2641 aatcacttca aagcagaaca cgaggcgatg ctgaagcagg cacaagatga accggcaatc
     2701 gatgagattt atcattttga ttttttggaa gcaaaagaac ttgggctgaa acataatagt
     2761 tcattttatt cgaaacatat gaatttttct tctagcgaac caaagacgga tctatttttt
     2821 ggtgcaacga ataatgggag aaaagaacga gcagaaagct ataagcagga atttataaaa
     2881 agagatgtta cgaccaacta tttcattctt ccacatcgag ggaatgaaca acctggatat
     2941 cttacttatg aagagtacct gaaaaaaaca ggggaatcgc gcgggattct ggaactgttg
     3001 cgggagggtc agcaaggagt gactttgcgg acatttgaat ccatgtattt tcaaaagaaa
     3061 ctagtgacgg ataatcaagc gattgctcat tatcattttt atcgtcctga aaatattttt
     3121 ttgcttcgag aacgtgattt agatgaatta ccggaatttc tgcatacacc atttaagcca
     3181 atcctatcag agctattgga attttatgat gcagaaaatt gggcaaaacg atttatcact
     3241 aacgattcca ctatttatga gcaatacgaa tatgacctct cctctgttga aaacaaataa
     3301 aaaacctaca ttttgaagag attatgtact gaaacaaaca agcacaattc ttatggcatc
     3361 atgagagttg tgttttcttt ttgatctttt attgggctta aaaaaagaga accagctgct
     3421 gattcagctg attctcatca gagagccttt tattctgcgc caaggattcc ttcttttgca
     3481 tggactaatt gggtcaaaaa ggcacaatat ggtgtagcaa tatcatattt ttgtcctttt
     3541 cgccaaatgg caccattgat gtagtcgatt tcagtcaaac gatggttttt gatcaggtct
     3601 tggtacatag atgggtaatg caacccgatg ttgtcaggat cataagtaga agcaatatgt
     3661 tggtacactt cttcttgatc tagtatgatt ccttcttttg ctgcgataga agcaaattca
     3721 gatacgattg ttcgaaccat gctctctgcc gctttttgcg cgcctagttc agcaatatta
     3781 caatccaaaa tcgtgcataa accattcaat gttccattta cacaagcttt tcgccaaatg
     3841 gaataacgaa cgttatggct atagatcgga tttaaatttg cttcttgaaa gacttctaca
     3901 actttttttg taaatgcttc gccttctggt tccaagttct ctagttcgat ttctccatca
     3961 cctaacaatg tgacttttcc gggaccagct agaccagctg tccacatcgt gatcccaaat
     4021 aagatatttt tctttggcac atatttttcc agtacatctt catgacctaa tccatttagt
     4081 aaacacaaaa cataagtatt gtcagtaata atggattgga tcgaacaaaa catatcatct
     4141 aattgattgg cttttgttaa tgctacgatc aagtccacgt gttcgttgct ttcgatgatt
     4201 tcttctggtg aatagattgg cagttttgcg accacttctt taccgttaaa gtcagcaatc
     4261 aacccatttg tacgaatggc ttcaatgtga gcaggccaac gatcgatcaa gaggacctca
     4321 tttccgcttt gatgtagcat caatccgatt cggctgccca ttgctccggc cccagctatt
     4381 gcgattttca tgttttttct ctcctttatt caatgagttc ccttttagta tatagaaaaa
     4441 tggaagaaaa tagcattgtt ttgtttgaaa aaaatgaaaa aaagtgtctt acttaagaag
     4501 aaagtgaggg atacagatgg atattttggc ggcaattgat tcatttaaag gaagtgcttc
     4561 ttcatttgaa ctaaatcagg cagttctaaa tggctgggaa gatcgtaaag gaagaaaaat
     4621 caatgtgccg attgcagatg gcggagaaaa tactgcagaa gcaatctatt acgcgctcag
     4681 gggaacatgg cgtaccattg atgggatgga tctcttattc cgtcagcgaa aaatcagcta
     4741 tttattgaca acttggcaag gaaaaaacac cgcagtcatc gaatctgcac aagcattggg
     4801 gttagatcta cttgataaac caacggatca aactatccga cttgcttcca gttatgcctt
     4861 aggagaactt attaaagatg cactgcttca agaagtagaa caaattattg tcactttagg
     4921 aggaagcggg tgtagcgatg gtggtctagg attactccaa tccttgggtg cttctttaga
     4981 agggtacaca gaaggaaatc ctttattatc tactaaaaaa cttcattttg atcgaagaaa
     5041 cgttcttgtt tctaaagtaa aattgattat cgctgcagat gttaccaatc catatacagg
     5101 tcaacaagga gctgcttggc aatttggaag acaaaaagga ggaatagata cgacgctagc
     5161 tttcttagat aaacaagcag gaaagattgc tcagcaaata aaagatttct gggaggtgga
     5221 tttagatgat attccaggaa gtggagcagc aggcggtatt ggtggagccc tttttttgtt
     5281 gggagcgaag atggtttctg gtttcgattt agtcagccaa atcactggat tggaaaaaaa
     5341 gatcgaacaa gcagatgtca tcattacagg agaagggcgg atcgatcagc aaacgatcca
     5401 cggaaaagtt ccatacggtg tagcagtgct tgcaaaaaaa tatcgaaaac caataatcgc
     5461 tatttgcgga agtagagacc aagagctaga aggactttct gattttctcc caattgtctt
     5521 aagtattcat ttgggtccaa taggtttaga agaagcaatg gaacacaggc aaacattaga
     5581 aaaaacaaaa atcttaggac agacacttag cagatttttc accatcctcc cgaagtagtt
     5641 gacaaaatca ttttctttat gctatcgtaa tttcaaatat aggacaaagt gagggatccg
     5701 tgcattgaaa aattaagtcg attgatgaaa gctgcattca gactgtacct tgtggagaca
     5761 aggaagcagt gtgcagattt tcgggattgg cttggttttt tcgatgtatg ggtctttttg
     5821 tgtgtatcca acccttgcat cctctttagc cagttctgaa aataggactg gtcttttttt
     5881 acgaggtgat ggaaaatgtc taaaatcgaa ataaaaaatc tgacattcgg ctacgacagc
     5941 caaggcacat tattatttga acaagcaaat ctaaattttg acacacaatg gaaactagga
     6001 cttatcggac gaaacggtcg aggaaagaca actttactga atattctaca aaacaaacta
     6061 ccttatcaag ggcaagtaat ccatcagcaa gaatttgcct atttcccgca acagacaaaa
     6121 gataaagaac gtttaaccta ttacgtgtta aatgatatta cggattttga gatatgggaa
     6181 atcgaaagag agctccaatt gatgcaaaca gatcctgaaa tcttatggag agaattcagc
     6241 acactatcgg ggggagagaa gacaaaagtc ctactggcac ttttatttgt ggatgacact
     6301 catttcccgt taatcgatga accaacgaat catttggata tctctggtag aaaacaagta
     6361 gcggcttatt tgaaaaagaa aaaacaaggc ttcatcgtgg tcagccatga ccggggattt
     6421 atcgatgaag tagtggacca tgttttagca atcgaaaaaa gtcaactgga actttatcaa
     6481 gggaatttct ctatctatga agaacagaaa aaacttcgtg atgaatttga aatggctcaa
     6541 aatgaaaaat tgaaaaaaga agtcagtagg ctaaagaaaa cagcagctga aaaagccgaa
     6601 tggtctcgtt cccgagaagg agataaaaca aagaaacaag tcggattcat cgatactgaa
     6661 tctagacgag tgaataaagg agcagtgggt gctgatgctg cacggacgat gaaacgatcc
     6721 aaagcaatcg tgaatcggat ggagacccag atcagcgaga aagaaaaact attaaaagat
     6781 atcgaatata tcgattcttt gacgatgaat agccaagcgt ctcaccataa gcgactttta
     6841 agcgtagaag atcttcaatt agggtatgaa aatctgttat tcgagccaat tcattttaca
     6901 atcgagcctc atcagcgggt ggcgatttca ggtcctaacg gtgcaggaaa gtcatccatt
     6961 atccattatc ttctgggggc attcaacggc aaggttatag gagaaaaata ccagccaaaa
     7021 catctgagca ttagttatgc aagccaaaat tatgaagaca atcgaggaac gttggcggaa
     7081 tttgcagaga aaaaccaagt agactaccaa gcatttttga acaacctccg aaagcttggg
     7141 atggaaagag atgtttttca taacaagatc gagcagatga gtatgggcca acggaaaaaa
     7201 gtggaattgg ctaaatcttt atcacagcca gctgaactat atacatggga tgaaccattg
     7261 aattatttgg atgtcttcaa tcaagaacaa ttagaacaac tgatcttgaa cgtgaaacct
     7321 gccatgttac tagtggaaca tgatcaaacc ttcctggata aagtatctac tgagattatt
     7381 tctcttgaga gaatctaacc cttgtaatct caagaacaac aaggtatggt taaggggaac
     7441 tttgccaacg aaggagagaa tcacatgctt tatgtaacga gacacggaga aacaacttgg
     7501 aacgcacaag gattagtttg cggacgggca gatgtgccgc tgactgaaaa aggacagatg
     7561 caggcacaga aactagcaga aaaggtagta gatttgcctg ttccaatcac gaaaatcatc
     7621 cattcccctc ttcaacgagc aagagataca gcacaagcag ttgctgatcg tctcagcttg
     7681 ccgctgaccg ttgatgaacg attggtagaa atggattttg gggattatga tggactgcca
     7741 agcaaagatg agaactttca aaaagcacgt ttagcgttcg ctgtacgatt cccaaatggg
     7801 gagtcagtct tagatgtgta tgcacggatc gttcctttat tgaaagaatg catcgaagac
     7861 gaagaaaatg tctatctgtt agtttgccat aatgccttga taagagtgat caatgcttat
     7921 tttcatccga tgcctaatga ggggttcttt acattcatgg tagataatac agaattggtt
     7981 tcttatgagt aattatgttt ttgtgttatt taaataaaat atttcaaggc actgcttttg
     8041 cttagcagtg ccttttttag gttatgacaa ttcttttgat aatataaact caacctttag
     8101 aaatgaaatt taattatttt aataaaagta ttgaaatttt gtttctttag atatatactc
     8161 tagtcataga aatgaagacg gatacagaaa aagagggata atatgtttgg cttttcaata
     8221 tttatgaacg attctttgtc agaagagaag aaacagtata tccgccaaat ggcatccaat
     8281 ggctttgtcg gtattttcac ttccatgcat ataccagaag atgatgtttc ggcatatcaa
     8341 aagcgattga tcgaactagg aagcgaagcg aaaaagaata aactagaatt gatggtcgat
     8401 atttcaggag aggcattgga acgagcgggt ttttcattag aagatctaac gcctttgaaa
     8461 gcaattggtg taacaggttt aagaatggat tatcatattt ccaatcagca gattgccgac
     8521 tggtcacatc aattgaaaat cagtttgaat gctagcacga tcacgcctaa agatatcgat
     8581 gaattgaaag aagcagaagc tgatttctca cagattgaag cttggcataa ttattatcca
     8641 cgtcccgaaa cagggctgga caaagaatgg tatcagaaaa aaaatcaatg gctcaaaatt
     8701 caaggtctcc ttgtccaagg ttttgtacca ggagatacag agttgagagg cccactatat
     8761 caaggattgc caactttgga agaacatcga gggatccatc ctcttgcagg cgctctagat
     8821 ctattagcaa gtaacacgga tattgtttac atcggagatg caggtctgtc agaaaacgtt
     8881 caggagcaat ttgcttcttt ccaaaaagaa cagacagtct tgctccatac ggaacctgta
     8941 gatgaagaat tttacgaata tatattaggg aagcatacga atcggcaaga cgatgcaaga
     9001 gatgtcatac gaagcgtaga tgcacgtttt cgagagattc cgcctatccc agcaagaaat
     9061 acagctacgc gtatgaaagg gtctattact ttagataacg aaaagtattt gcgctatatg
     9121 ggtgagatcc aattaaccaa atatgatttg ccagcggatg aaaaagtgaa tgtagtagct
     9181 aaagttatca aagaagagct accattgatc aaccaaatca aagctggtat gaactatcaa
     9241 tttataagaa aagaaggaag ataaaaatga gcaaaatcaa tttagagaac ctaacaacgg
     9301 aacgcagaaa ccaagaaact tttggattag atgagatgag tatttctcaa gccgttcaac
     9361 taatgaacaa agaagatcag aaagtggccg ctgcggttga aaaagaactg acaagtatcg
     9421 aaccagtgat tgctaacacg attgagtcct ttaaaaaagg tggacgtctg atttatatgg
     9481 gagccggaac aagtggaaga ctaggtgtgt tagatgctgc tgaatgcgta ccgacattcg
     9541 gtgtagaacc ggaaatggtc ataggtctaa ttgctggtgg agaagaagca atgactgtag
     9601 ctgtcgaagg ggccgaagat tcttatgagc tagggcaaga agatctaatc aagctgaatt
     9661 tgacaaaaaa tgatatagtt attggtatcg cagctagcgg tcgtacacca tatgtgatcg
     9721 gtggattaga ttacgcaaaa tcgatcggtg ctgtcacagg aacgatttct tgcaataaac
     9781 aagccgaaat cagtaaacac gcggatttcc caatcgaagt cgatgcaggt ccagaatttt
     9841 tgacaggctc tactcgattg aagtcaggaa cagcacaaaa acttatcttg aatatgattt
     9901 ctactatcag tatgattggt ataggtaaag tattcaataa cttgatggtc gatgtcaaac
     9961 caacaaatga aaaactaatc gaacgctcaa aacgaatcat catgcaagcg acagaagctg
    10021 attacgaaac agctgaaaag tactttgaag aagcagatca aaatgttaag ctggctatcg
    10081 tcatgatttt gacaaatagt gataaagagg aagctcaagc aaaattagct gaagcaaacg
    10141 gatttgtaaa agggacctta tgagggggaa aagaccatgg caaaaaacga actgactttg
    10201 gaagaactgg caaaacaaat ttataaaggg gcaggcggca tgggcaatgt agaaagcatt
    10261 gtccactgca tgactcgtgt gcgtatgaag atcatcaatg atgatcttgt aaatgtctct
    10321 gaattaaaag cgattccagg agtgcttggt gtagtagaag atgaacagct tcaagtcatc
    10381 atcggacctg ggaaagtgaa caaagtagca cagcaaatgg ttgatttggc aggcgtgaaa
    10441 ttaggagaaa aactttcttc tgtaagtaca gctacagcaa attcatcagc atctggaaaa
    10501 gaccgtgtaa acgaacgggc acaagaaatg aaagcggcac aaaaatcgaa acaaaaacca
    10561 tctaaaatca aatcggtatt aaaagacatt tctaatatct ttgtgccaat gattcctgca
    10621 tttgtcggta ctgggatcgt ggcagggatt gccgcagttt tagcaaactt agtcacagcc
    10681 ggaacacttg atgcagcaac atggcaacaa tatatcgatg tgatgaacat cttgaaaaac
    10741 gggatgttta gctacctagt tatctataca ggtatcaatg ctgcacaagt ttttggtgca
    10801 acaccaactc ttggtggagt tatcggggcc gttgtgatgc tgacagggat gaatccggaa
    10861 gcaccactga aaaacttatt tactggtgaa gctcttgcag ctggacaagg tgggatccta
    10921 ggtgtcatct tcgctgtttg gctattgtca atcgttgaga aaaaactgca taaagtagta
    10981 cctgatgcgg tagatatcat cgttacacca actctttcat tgattgccat cggtctgatt
    11041 gaaattttct tcatcatgcc tttagcaggt ttcatttctg atggcatggt cggtgggatc
    11101 aactgggtac ttggcgttgg tggagcattt gccggcttcg ttctaggcac attattcttg
    11161 ccgatggtta tgttcggact gcatcaaatt ttgactccaa tccatgtgca aatgattgat
    11221 gaaactggtc gtacgctatt gcttccaatt cttgcgatgg ctggtgcagg acaagtgggt
    11281 gcagcattgg cattatggat tcgctgcaaa aaagataaag aattaacaga aatgatcaaa
    11341 ggagctttac cagtcgggat cctaggaatc ggcgaaccat tgatttacgg agtaacatta
    11401 cctctaggac gcccatttat tactgcttgt atcggtggag gtatcggcgg ggcagtgatc
    11461 ggaatgatcg gaaatgtcgg ggctatcgct attggacctt caggagccgc tttgatccca
    11521 ttgatctctg acggtaaatg gttaggctac atccttggat tattggcagc ttatgctggt
    11581 ggcttcgttg ctacattctt cttcggtatt cctaaagacc gtttagaaaa agaagaatta
    11641 gcgactgaaa cagccacagt aaatgaagtg cctgaaaaag tagtacctca aacttcagct
    11701 gaaagtgtga ctatcctctc atcagttgct gacggaacag ttgtttcttt agataaagca
    11761 agtgatccag tttttgcaca aaagatgatg ggtgaaggct actttgtaga accagaaaac
    11821 ggacatatct attctccagt tactggggta gtcagctctg tcttcccaac gaaacacgct
    11881 attgggatca caacgccaaa tggtttagaa atcttgcttc atatgggaat caacacagtt
    11941 gacttaggcg gtacgccatt tgatgtgaaa gtaacagaag ggcagcaagt gacacctgat
    12001 acgcttgtag cagaagctga cttagaagcg atcaaagcag caggcaaaga aacatccatg
    12061 atcgtcttgg taaccaatat ggatcgtgta aaaaactttg tcttggaaaa aacaggtaaa
    12121 gcaaaagcaa aagcacctgt gatggatgtc aaagcttaat atgatagaaa caatagaaac
    12181 gaaagaagaa actgaaatct atttttcagc ttcttctttt ttattattta ttgttttctg
    12241 ctaaaatctg ataaattcgt gtcacatcag atggtgttcc ccgtaattca taatctgcca
    12301 agaaaggaaa gccaaatgtt tgtgcatatt gtttggctgt tagacaatac tgattattga
    12361 agtttttatt tccgctaccg actacaccaa gacaaagttt atgattgtct tcatgttcca
    12421 gatattcacg catcgtttcg gtcaagattt ctgtatcacc gttatctaaa ccatttccgc
    12481 cgtctaaata agtaggaacg aaagtaaaaa atggttctgt ttctttcgta aaatcactat
    12541 tttcatggat ttcttttaaa gtaacttcag gattgacagg atcttctgca tgcatttttt
    12601 cagcatattc tgctagatgt ttggcaaagg cacgcgtatt tcctgaaatc gatatatata
    12661 aaatgttcat ggatcatcct ccaattcttt ccttttagta tagcatcttt tttaagtttt
    12721 ttggtggtgt aatttggaaa gggactaggc gctttgcttg tataatgaga aaaaagccgg
    12781 tagaattata tttctattaa agtttttatt aattaagtaa aaataaaaaa ggtttgttat
    12841 actttttgat aaaagcggtt ttattttgga ggatgcttac atggatacag agcaacagaa
    12901 aaaaggaatt ggtttaattc cacttgctgc cttagtaatt ggttcagcga ttggaggtgg
    12961 cgtatttggt attatgacgg acatctccgg atcggctggc ggcccagcta ttttcagttg
    13021 ggtactcgta ggaacagcaa tgttgatgct ctccttgtcg atcaataata tcaataaaaa
    13081 acgtccagat ttaaaaggcg ggatctttag ttacgcagaa gaaggttttg gccgtttcgc
    13141 tggttttatc agcggttggg gttactggct cacatgctgg ttaggcaacg tagcttttgc
    13201 aacattgttg atgagcgcat taggatattt cttcccagta tttgaaggtg ggcaaaatct
    13261 ggcatcgatc gtgatgagta cagtcttttt atggggatat gcatggctag tgaatcgggg
    13321 agtcgaaaat gctagtttcg tcaatgctat cgtcacattc tgtaaattag tgccattatt
    13381 tttatttatt gtagtagcta tcagcacgtt ttcgatccaa caattcattg ataacttttc
    13441 tagcgacatt atattgaatg cgaatggaca gccgatcccg ttttccagtc aagtattgag
    13501 ttgcatcatg gtgatgcttt gggtatttgt tggtattgaa ggagcctctg tcgtaggttc
    13561 acgagcaaaa aaacaatcag atgttgggaa agcaacgatt ctagggatat tcggcttgat
    13621 tattatttac gtcttggttt ccatgcttcc atatgggaca atgacgatga atcagctgca
    13681 aacgatcaag gcgcagcctg ctatgggcta tgtgtttgaa atgatggttg gtaaatgggg
    13741 agcagtcgtg atcaacggcg gtttgatcat ttctttagtt ggtgtttggc tctcatggac
    13801 gatcttaccg attgaaacaa tgggaaacat ggctgaagac ggacttttgc ctaaagcatg
    13861 gggaaaactc aacaaaaatg gggcaccaac ttatgctatc gtattgacga cgttatgtac
    13921 gaatgtcttt ttactttcat tattattcac tgacagtgct tataactttg cttacacact
    13981 tggtacagca gctattttct tcacgtggct tttcttagga ttgtaccaaa tgaaactttc
    14041 ttatcagcgg aaagaatggg tccaattttt agtagggctt ttagcaagtg cctttcaagt
    14101 atgggctatg ctagtcgtag cctttaatga agtaatgctt gttcttgttc ttttcttgcc
    14161 aggcatttat ttttacctca aagcacgtaa ggagcaaaac gagcctgtac aacatatcca
    14221 cttagataag attttactta taggtgtgac gatggtcgga gtgattgcaa tggttttatt
    14281 tgccactggg atgcttcacg tatagaagta taaaagaagt ataaagagag gttgtgagcc
    14341 tgtcgtttat tcaaaaacat actaggaaag gagctgtgac acaaatgttg tcacagctcc
    14401 tttttcagaa taaacggtgt ttaagagaca gccaatcggc aataaggctg aacgtctctt
    14461 tcacagcctc ttttttgatg agtcatcata cattttttcg cttaaatacg agatatccta
    14521 atgctaagaa caaaacgatg taagctaaat taccgaatag catttgatta gtagaaagat
    14581 gagtcaattg cttcatgatc tcttcgtttc caatttggtt ttgcagattc aacatactga
    14641 tcggattcca tttgatccac tcccattttt ggatgagtgc aataaggata ccagatatga
    14701 ttgacgaagc aaaataaaag acgattcctg ctgagatgga tgcgccggaa ctattgataa
    14761 agcaagcaat catcaacact aaacttaaaa tcagccataa accgatataa ctacccaaag
    14821 tataagtgaa caagctttgg atcagtgttt gaccagtaga aaccttctcc gtgaaggaaa
    14881 tcgaagggaa aagaatcagt ttcatcaaca cagtgacaat aatagctaaa aggtataaat
    14941 aaacagaata aataactaat gtgatccatt tgcttactaa tatttctcct cgagaatact
    15001 ttcgataaag gagatttttc aatgtaccat attgtgcttc catcgaaatg atcgtgcttg
    15061 cagctgcaat catgataaag acaatccaag atgtggcact aaaaagctga gcagtcattt
    15121 caactggatc aatgatcgtc gtgtattttt tggctagaag tgctgttcct atgagcaaaa
    15181 caactaatag aacagataaa acagcagttg actttttctt gatcaattta aataattctt
    15241 gttttacaag caatgtcatt tttcttcttc cccctttact gaatccagaa tttccaatag
    15301 agaagattcc aaatcattcg tcagatgctg gacgtctgtc acatggatat tttgtgcaga
    15361 caatagctgg atcacggttc ctaataattc ttcgttttct tttaaaagct tgattttatc
    15421 aggatcttcc aaagtatatc cagcttctgt caagatcgtt cgagcagcta gatcatttgt
    15481 tgtagagata acgatgaaat cttgcgctgt ttggttcaat tcagccattg tacattgttt
    15541 gatgatcttt ccttgattga tcaggatcag ctcatcgacc attttttcaa gctcgctgag
    15601 taagtgactg gagatcaaga aagaggtgcc ctgagtagcc aagtcggtaa tgatttttcg
    15661 cacatctctt gttgcttgtg gatccaaacc gttcatcggc tcatctaaaa taacgataga
    15721 cggatggttc agtaaggcaa gagcaattcc caatttttgt ttcattccca acgagtagga
    15781 tttcgctttt ttgtgaatgt acttttccat cttgagctga ttcacgatag tgtcgatagc
    15841 agatacatcg ttggtttcag agaataactt cagatgatca tagcctgtta agaatggata
    15901 gatacccggg tattcgatca atgctcctac ttgttccaac cctttatgac tagagggaga
    15961 aatctcattt tcaccaatac agattgttcc atctgagtaa tggatcagtc ctaaaatagc
    16021 tttcatgatc gttgtttttc cagcgccatt aggacctact aatccaacga ttttgccagg
    16081 ggataagcta aaactgacat cgctgagggc ttgtttgtga ccaaagttct tggataattc
    16141 ttggaccgaa agacttttca aaaatattca ctccttttct atagatagta caagcataca
    16201 cctgatgagt ggagaagtcg ctaggtcatt ggacttattt cttatttttt cataaaaaaa
    16261 ttgaatatta aataaaaagg cttatttttt ctgatcagta cttattccaa gtgtctagtg
    16321 gccgctagta atcttgatgc caatgatact gatgagtagc atccccacaa agaaccaagt
    16381 ggaaagattc agcttatcat gaaacaagac cacaccaatt atgaccgaac caactgcgcc
    16441 aatccctgtc cagataggat aagagagact catcggcaaa cttttgattg cctttgacaa
    16501 aaagtaaaaa ctagcaatca tgccaatcac tgtgtaaaaa gaataattga ttttagaaaa
    16561 accatcactc catttcatca tcgttgacca gaatacttcc aaaattcctg ctatgattaa
    16621 ttccatccat gccatactta cattcctcct taactataaa aaaatacaca aaaagaggaa
    16681 aatcatccct tcaaagacct aatggaatga tcatcctctt tatcctaccc ggtggcagat
    16741 tgtatatctt tttctgtagt atagcacacc tatccagata tcggcaatct catacttttt
    16801 agtaaagtgt accaagcact gacggatata tcgttagcgg aaaagaagat gaatgctttg
    16861 caatttcata gaaagaagtt gttatttatt aatcatttaa aaaaaacagt aactagacct
    16921 tatttctata tattat
//
LOCUS       DARBET010000244         8061 bp    DNA     linear   BCT 07-NOV-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4027
            SAMEA114569841-rid20112183.guided.101, whole genome shotgun
            sequence.
ACCESSION   DARBET010000244 DARBET010000000
VERSION     DARBET010000244.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA114569841
            Sequence Read Archive: ERR12203666
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 8061)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8061)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001972618.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001972618.1
            Genome Coverage       :: 132x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/07/2023 13:18:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,041
            CDSs (total)                      :: 2,975
            Genes (coding)                    :: 2,908
            CDSs (with protein)               :: 2,908
            Genes (RNA)                       :: 66
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 67
            CDSs (without protein)            :: 67
            Pseudo Genes (ambiguous residues) :: 0 of 67
            Pseudo Genes (frameshifted)       :: 26 of 67
            Pseudo Genes (incomplete)         :: 28 of 67
            Pseudo Genes (internal stop)      :: 20 of 67
            Pseudo Genes (multiple problems)  :: 6 of 67
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8061
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA114569841-rid20112183.guided.101"
                     /isolate="VRE4027"
                     /db_xref="taxon:1352"
                     /country="Denmark"
     gene            128..1564
                     /locus_tag="SCJ86_003013"
     CDS             128..1564
                     /locus_tag="SCJ86_003013"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289243.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 1 protein"
                     /protein_id="HEG1170479.1"
                     /translation="MEHKELTSFPKDFLWGSASAAYQVEGAWQEDGKGESVWDRFVRI
                     PGKTFKGTNGDLAVDHYHRYKEDIALMKEQGLKAYRFSVAWTRIFPNGRGEVNQAGLA
                     FYERLIDELIENDIEPMLTLYHWDLPQALQDEYNGWESRQIIEDFTHYAETLFEAFRG
                     KVHYWISLNEQNIFTSLGYLLAAHPPGVTDPKRMYEVNHIANLANASVINKFHELEMP
                     GKIGPSFAYTPNYPIDSNPENVLAAENAEDLMANYWMDVYMWGKYPIAAMRFLEEKGW
                     APTIEAGDAELLESAKPDFLGINYYQTATNAFNPLNGVGAGKMNTTGKKGSSEETGVP
                     GMYKKVENPFVERTNWDWEIDPEGLRIGLRRITSRYRMPVLITENGLGEYDKLTEDKQ
                     IHDDYRINYLQSHVKAIKEAISDGAEVLGYCTWSYTDLLSWLNGYQKRYGFVYVDQDE
                     TQEGSLERYKKDSYYWYQKLIEENGENL"
     gene            1753..4200
                     /locus_tag="SCJ86_003014"
     CDS             1753..4200
                     /locus_tag="SCJ86_003014"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344000.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA translocase FtsK"
                     /protein_id="HEG1170480.1"
                     /translation="MAQKKRPTKKRKKTKKTKKQKQQQEQMLTIGVGFALILFAVFGF
                     LKLGFLGILIANGFRIIAGNTYQILCLLLAVLGFWIVIKNTEFSIGKNRRWFGGILFY
                     FGILLLLHAHLFGKLHTGEPNIMGTTWDLLASDIKQSQVDNNVGGGMIGAILYHFTYF
                     LIAQPGSYLAAVLLLAGGAFLFSNLEGYQLLNGIQSIGERVQELLEGDPAKQARKQAA
                     KEERMKQRAEAKEARRLAAKEAAEKEAAEYEKKKAVSQQRENTADEWQEPENTEPEQL
                     SFVPIDSFQENIHPANLEKPVPDTPKQTNTAEGFSDELPEDDGTSLEFEIEAEQENQD
                     YELPTVDLLDSIPTVDQSDEYKKIEKNIGVLEQTFQSFGVDAKVVKASLGPSVTKFEV
                     QPAVGVKVSKIVNLTDDIALALAAKDVRMEAPIPGKSLIGIEVPNGKISMVSFREIIE
                     AQPNHPDKLLEVPLGRDVSGRVQTADLSKMPHLLVAGSTGSGKSVAINGIITSILMRA
                     KPHEVKLMMIDPKMVELNMYNGIPHLLTPVVTNPRKAAQALQKVVQEMEERYEKFAAT
                     GVRNISGYNEFVQQKNLENGTKHPTLPFIVVIVDELADLMMVASNEVEDAIIRLAQMA
                     RAAGIHMILATQRPSVDVITGIIKANVPSRMAFAVSSGTDSRTIIDSNGAEKLLGRGD
                     MLFLPMGENKPIRVQGAFISDHEVERVVQFVTDQQEAHYEEKMMPTDEVETTGAPEQP
                     QDELFEEAKALVVEMQTASISLLQRRFRIGYNRAARLVDELEAHGVVGPSEGSKPRKV
                     FIEQEETSETLPDDNDM"
     gene            4430..5497
                     /gene="rlmN"
                     /locus_tag="SCJ86_003015"
     CDS             4430..5497
                     /gene="rlmN"
                     /locus_tag="SCJ86_003015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629547.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (adenine(2503)-C(2))-methyltransferase
                     RlmN"
                     /protein_id="HEG1170481.1"
                     /translation="MEKQSIYGLTNEELINWFIENGEKKFRAAQVWEWLYQKRVSNFT
                     EMTNLSKQLIEKLSEHFIINPLKQMVVQEASDGTVKYLFELPDKNMIETVLMRQEYGL
                     SVCVTTQVGCNIGCTFCASGLLKKNRDLTAGEIVAQIMMVQHYFDERNLGERVSHVVV
                     MGIGEPFDNYDNVMDFLHIINDAKGLAIGARHITVSTSGLAHKIKEFANNGLQVNLAI
                     SLHAPNNEVRTSIMRINRSFPIEKLMEAVDEYLEKTNRRITFEYIMLNQVNDRPEHAQ
                     QLADLLKDKKKLAYVNLIPYNPVSEHDQYSRSPKADVLRFYDVLKKNGVNCVIRKEHG
                     TDIDAACGQLRSKQMKKTVAK"
     gene            complement(5690..6793)
                     /locus_tag="SCJ86_003016"
     CDS             complement(5690..6793)
                     /locus_tag="SCJ86_003016"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373950.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-containing alcohol dehydrogenase family
                     protein"
                     /protein_id="HEG1170482.1"
                     /translation="MSQSVFLPNYTIGTDAYEKANGIVKEYGTKVVLIGGKTALSKAE
                     NKIKVALTQEIEVVASLWYGGNSTYGNIEQLIQHESVQKADVLFAVGGGRACDTVKVI
                     GEKLAKPVFTFPTIGSNCSPVTAVCVIYNEEGVMDGLFFPSKPPVHTFIDTAIIAEAP
                     DKYLWAGIGDALSKEVESTFSARGDDLGYTDRLGIQIGKMCIDRLLTYGTQALKDSAD
                     DRPSYAIEQVVSDIIVTTGLVSVLVNNNYNSAMAHSFYYGTTVLEKMEGYLHGASVSY
                     GVLTLLMMDQQFDLFDKIYVFMKKNQLPVSLTDMNLNVTTDLEAILDKAMATNDIKHV
                     PYEITREKFKQAILDVETYHATQESQQGYKKIS"
     gene            7346..7894
                     /gene="aac(6')"
                     /locus_tag="SCJ86_003017"
     CDS             7346..7894
                     /gene="aac(6')"
                     /locus_tag="SCJ86_003017"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008265821.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminoglycoside N-acetyltransferase AAC(6')-Ii"
                     /protein_id="HEG1170483.1"
                     /translation="MIISEFDRNNPVLKDQLSDLLRLTWPEEYGDSSAEEVEEMMNPE
                     RIAVAAVDQDELVGFIGAIPQYGITGWELHPLVVESSRRKNQIGTRLVNYLEKEVASR
                     GGITIYLGTDDLDHGTTLSQTDLYEHTFDKVASIQNLREHPYEFYEKLGYKIVGVLPN
                     ANGWDKPDIWMAKTIIPRPNSQ"
BASE COUNT         2696 a         1375 c         1751 g         2239 t
ORIGIN      
        1 tttcttacta ctcgaaacta agcgactttt tcacaacctc ctttacataa ttttattttg
       61 ttgtggaagg ggtttcgtgt tattctaaaa gaggaacgat tgaaggttat aggaaaggaa
      121 gagatgaatg gaacacaaag aacttacctc atttccaaaa gattttctgt ggggctctgc
      181 ttctgcagcg tatcaagtag aaggagcttg gcaagaagat gggaaaggag aatcagtctg
      241 ggatcgattt gtacggatac ctggaaagac atttaaagga acgaacggtg atttggcagt
      301 agaccactat catcgttata aagaagatat tgctttaatg aaagaacaag gtctgaaagc
      361 ttaccgcttc tctgtagctt ggacacggat ttttcctaat ggacgaggag aagtcaatca
      421 agctgggctc gctttttatg agagattgat cgacgaattg attgaaaacg atattgaacc
      481 tatgttgact ttatatcatt gggatcttcc acaagcattg caagatgaat ataatggttg
      541 ggaatcacgt cagatcattg aagatttcac tcattatgca gaaacattat ttgaggcatt
      601 ccgcgggaaa gtccattact ggattagttt gaatgaacaa aatatcttca cttcattagg
      661 ttatttgtta gcagcacatc cgccaggtgt gacagacccg aaacgaatgt atgaagttaa
      721 ccatattgct aatttagcaa atgcatctgt catcaataaa tttcacgagt tagaaatgcc
      781 tggaaaaatc ggaccaagct ttgcgtatac gccaaattat ccaattgaca gtaatcctga
      841 aaatgttctg gctgctgaaa atgcagaaga cctgatggcc aattattgga tggatgtcta
      901 tatgtgggga aaatatccga ttgctgccat gaggttctta gaagaaaaag gatgggcacc
      961 aacgattgaa gctggcgatg cagaattgct agaatctgct aaaccagatt ttcttgggat
     1021 caattactat caaacggcga cgaatgcatt caatccatta aacggagttg gtgctggaaa
     1081 aatgaatacg acaggaaaaa aaggttcatc agaagaaact ggagttcctg gaatgtataa
     1141 aaaagttgag aatccatttg tcgaacgaac aaattgggac tgggagattg acccagaagg
     1201 attaaggatc ggactgcgtc gtatcacaag ccgttaccgc atgccggtac tgattacgga
     1261 aaatggctta ggagagtatg acaagttgac agaagataaa caaatccatg acgactatcg
     1321 aatcaattac ttacaaagcc acgtcaaagc catcaaagaa gcaatttcgg atggagcaga
     1381 agtcctaggc tattgcacct ggtcttatac agatttgctt agctggctga acggttatca
     1441 aaagcgatat ggatttgtat atgttgatca agacgaaaca caagaaggat ctttggaacg
     1501 atataagaaa gacagttatt actggtacca aaaactgatt gaagaaaatg gagagaacct
     1561 ttagttcaca tacttaaaac aagaaggtac ggctggatat ggccgtgcct tttcttgata
     1621 aaatcattta gaaattaaga aaaactgtaa aataagctga aaaatgaaag aaagcgtcag
     1681 ttctttcaaa caaaacttta gttcccttac ttttttttgt ataatgaaac ttgatgaaat
     1741 gagggataat aaatggcaca aaagaaacgg cctactaaaa aaaggaaaaa gacgaaaaaa
     1801 acgaaaaagc aaaaacagca gcaggaacaa atgctgacga ttggtgttgg gtttgccttg
     1861 attttatttg ctgtcttcgg ctttttaaaa ttaggttttt taggaatatt gattgcaaac
     1921 ggctttcgga tcattgcagg aaatacttac caaatcctct gtcttttatt agccgtatta
     1981 ggattttgga ttgtcattaa gaatacagag ttttcaattg gtaaaaaccg acgctggttt
     2041 ggcggcatac tcttttattt cggtatctta ttattgttac atgcccattt atttggcaaa
     2101 ctccatactg gggaaccaaa tatcatgggg acgacatggg atttgttagc cagtgatata
     2161 aaacaaagtc aagtagacaa taacgttggt ggtgggatga ttggagcaat cctttatcat
     2221 tttacttact tcttgattgc acagcctgga agttatttag cagcagtgct attactggca
     2281 ggaggagcat tcctgttcag taatctggaa ggctatcagc tgttaaatgg tatccaatca
     2341 attggagaac gagtacagga attattggaa ggtgatcctg ccaagcaagc tcgaaagcaa
     2401 gcggcaaagg aagagagaat gaaacagcgc gcagaagcga aagaagcaag gcgcttagct
     2461 gcaaaagaag ctgcagaaaa agaagctgca gaatatgaga agaaaaaagc ggtgagccag
     2521 caaagggaaa atactgctga tgaatggcag gaaccagaaa atacagagcc ggagcagcta
     2581 agttttgttc caatcgacag ttttcaagaa aatatccatc ctgcgaatct agagaaacca
     2641 gtaccagata caccaaaaca gaccaataca gcagaaggtt tttcagatga gttgccagag
     2701 gatgacggta cctcattaga atttgagatt gaagcagaac aggaaaatca agactatgaa
     2761 ttaccgacag ttgatttgct tgattctata ccaactgtgg atcaaagtga tgagtataaa
     2821 aaaattgaaa aaaatatcgg cgtattagaa caaactttcc aaagtttcgg tgtcgatgcc
     2881 aaggtcgtca aagccagctt aggaccatcc gtcacaaaat tcgaagtcca gccggctgta
     2941 ggggtaaaag taagtaaaat tgtgaactta acagatgata tcgcgcttgc tttagcggcg
     3001 aaagatgtcc ggatggaagc accgattcca ggtaaatcac tgattggtat tgaagtacca
     3061 aatggaaaga tcagtatggt ttcctttaga gaaatcatcg aagcacaacc aaaccaccca
     3121 gataaattac tagaagtacc tttaggacgt gatgtttctg gacgggtaca aacggctgat
     3181 ctttcgaaga tgcctcactt actcgtggct ggttcgactg gtagtgggaa gtcagtagca
     3241 attaatggga ttatcacaag tatattaatg cgtgctaagc cccatgaagt aaaactgatg
     3301 atgatcgatc cgaaaatggt tgaattgaat atgtacaacg gcatacctca tctgttgacg
     3361 ccggtcgtga cgaaccctag aaaagcagcg caagccttac aaaaagtagt acaagaaatg
     3421 gaagaacgat atgaaaaatt cgcggcaacc ggagtaagaa atatttctgg ttacaatgaa
     3481 tttgttcaac agaaaaactt ggaaaacgga acgaaacatc caaccttacc attcatcgta
     3541 gtgatcgtag atgaattagc tgatttgatg atggtagcca gcaatgaagt tgaagatgcg
     3601 attattcgtt tagcgcaaat ggctcgggca gcggggattc atatgatcct agctactcaa
     3661 cgtccaagtg tggatgttat cacaggaatc atcaaagcaa atgtaccttc cagaatggca
     3721 tttgcggtat caagcggtac agattctcgg acgatcattg attctaatgg ggcagaaaag
     3781 ctcttagggc gaggagatat gctattcttg ccaatgggtg agaataaacc gatccgtgtg
     3841 caaggagcat tcatttctga ccacgaagta gaacgggtcg ttcaatttgt tacagatcag
     3901 caagaagctc attatgaaga gaaaatgatg ccgacagatg aagttgaaac gacaggtgct
     3961 ccagaacagc cacaagatga gctgtttgaa gaagcaaaag cacttgtcgt tgaaatgcaa
     4021 acggcaagta tttcccttct ccaaagaaga tttcggatcg gttacaaccg agcagcacgt
     4081 ttagtagatg aactggaagc tcatggagtt gttggcccgt cagaaggaag caagcctcgg
     4141 aaagtattca ttgagcagga agaaacatca gaaacgttgc cggacgataa tgacatgtaa
     4201 aacttgtgtg atgaagggtt gaaaaacgaa taaccaataa aaagacaagg catgatgata
     4261 ccaaaagaaa aaatggtttt tttccatctt ttctaatacg gtatgatgtc ttgtcttttt
     4321 agtttttgtg agattaaagt tctattcgat ttatttgtga agtattgcat taaggaaatg
     4381 aatcatgtat agtatgtagc gaaactttga gaaaaagagg gaaaatactt tggaaaaaca
     4441 gtccatttat ggcttgacga atgaagaatt aatcaattgg tttatagaaa acggcgagaa
     4501 aaaattccga gctgcccaag tttgggaatg gttgtatcaa aaacgtgtaa gtaatttcac
     4561 agaaatgacg aatctatcga aacaattgat cgaaaaatta tcggagcatt ttatcatcaa
     4621 tcctctaaaa cagatggtcg tccaagaagc ttcggatggt acagtaaaat atttgtttga
     4681 actccctgat aaaaacatga ttgagactgt actgatgaga caagaatatg gcttgtcagt
     4741 ttgtgtgacg acacaagtag gatgtaatat cggctgtacg ttttgtgcaa gcgggctgct
     4801 caaaaagaac cgtgatctga ctgctggaga aattgttgca cagatcatga tggtccaaca
     4861 ttattttgat gagcgcaatc ttggcgaacg cgtttcacat gtcgttgtaa tggggatcgg
     4921 tgagccattt gataattacg acaatgtaat ggactttttg catatcatca atgatgccaa
     4981 aggattggca attggtgctc ggcacattac agtttccact agtggattag cacacaaaat
     5041 caaggaattc gctaataacg gcttgcaagt caatctggct atttccttgc acgcgccgaa
     5101 taatgaggtg cgaacatcga tcatgcggat caaccgaagt ttcccaattg agaaattgat
     5161 ggaagctgtg gatgagtatt tagagaaaac aaaccgtcga atcacgtttg aatatatcat
     5221 gttgaatcaa gtaaatgata gaccagagca tgcgcaacaa ttagcagatc tattaaaaga
     5281 taagaaaaaa ttagcatatg tcaacttgat tccatataat ccagtcagtg aacatgatca
     5341 atattctcgc agtccaaaag ctgatgtatt gagattttat gatgtattga agaaaaacgg
     5401 cgtaaactgt gtgatacgta aagaacatgg tacagatatt gatgcagctt gcggacaatt
     5461 gcgaagcaag caaatgaaaa aaacagttgc caaataatcg tatcctactc gttttttcaa
     5521 ttaccataaa aggagcagtt ttcttaatta aggaactgtt caacttaaat aagagaaaag
     5581 attctatagc gaaagctata aagctttttt tgacaagaaa aaccctcatc aagcaaagaa
     5641 atgtcctcaa tttgatgaca gcctgcttga tgggggtttt agcaattatt taactgattt
     5701 tcttatatcc ttgctggctt tcttgtgtag catgataagt ttctacgtca agaatggcct
     5761 gtttaaattt ttctcgggtg atttcataag gcacatgttt aatatcattt gttgccatgg
     5821 ctttatctaa gattgcttct aaatcggtcg tgacattgag attcatgtcg gttaacgata
     5881 ctggaagttg gttctttttc ataaacacat agattttatc aaacaaatcg aactgctgat
     5941 ccatcatcaa taaggtcagt acaccataac tgacagatgc tccgtgaagg tatccctcca
     6001 ttttttctag aacagtcgta ccgtaataga atgagtgtgc catagcagaa ttatagttat
     6061 tgttaactaa aacagaaacc aatccggtag taacaatgat atccgaaacg acttgttcaa
     6121 ttgcatatga gggacgatcg tcggcactat ctttcaatgc ttgcgtacca taagtcaaga
     6181 gacgatcaat gcacattttt ccgatttgga ttcctaatct atccgtgtag cctagatcat
     6241 cgccacgagc agaaaaagta gattcgactt ctttggataa agcatcgcca atacctgccc
     6301 ataagtattt atcaggtgct tcggcaataa ttgctgtatc aatgaaggtg tgtacaggtg
     6361 gttttgaagg aaagaagagt ccgtccatta cgccttcttc gttatagatg acacataccg
     6421 cagtgacggg cgaacagttc gaaccaatag tgggaaaggt gaagactggt ttagctagtt
     6481 tttcaccaat gacttttaca gtatcgcaag cacggccacc accgactgca aacaggacgt
     6541 ctgctttttg gacactttca tgctggatca actgttcaat atttccataa gttgaattac
     6601 ccccgtacca gagactggcg acaacctcta tttcctgtgt caaagctact tttattttgt
     6661 tttctgcctt tgaaagtgca gtcttccctc caatcagcac tacttttgtc ccatattctt
     6721 tgacgattcc atttgctttt tcatacgcat ctgttccaat ggtataattt ggtaagaaaa
     6781 cactttgact catattcatt tccttctttc ttcgttgaaa cgggttaaaa ttttaaagag
     6841 tgatcgatct gactgcggct gtagatggag tagaggtgaa cctaagacag gataagtgat
     6901 gaataaggtg gcatgaacac cttgaaatca tcaataagta tcctaaaaac gaacaagtac
     6961 aaaaacattg aaaacgacaa taatgaattt ttgttcctct tagtatttct accgattccc
     7021 tttgcttttt tgtaattatc taaaaattct aaaaattaag attattatat gaaaacaggt
     7081 tttatttgtc aattcataat tggttttttt taaagcaaaa gataatctat atcatgatga
     7141 acaaacagag ttttttcctc caattgatgg ctttcctgat ctttcctatt ttgaataaag
     7201 tgtttctgta taattttttt attttataag taagataaat aatgaaagaa tagagatact
     7261 gattggtagc taaagatttt tgagcgagaa catttcgttt tttcaaaagg actgttagaa
     7321 taagcaagaa caagggggaa gacgtatgat aatcagtgaa tttgaccgta ataatccagt
     7381 attgaaagat cagctttctg atttactgag actgacttgg ccggaagaat atggagacag
     7441 ctcggcagaa gaagtagaag aaatgatgaa tccagaacga atcgcggtag cagcggtaga
     7501 ccaagatgag ttagtaggat ttattggtgc aatccctcaa tacggtatca caggttggga
     7561 attgcatcca ttagttgtag aaagctcccg acgaaagaac caaataggta ctcgattagt
     7621 caattactta gaaaaagaag tagcttccag aggaggaatc acgatttatt taggtacgga
     7681 tgatttagac catggaacaa cgttaagtca aacggacctg tatgagcata catttgataa
     7741 agtggcttcc atccagaacc ttcgtgaaca tccgtatgaa ttctatgaaa aattaggtta
     7801 taaaatcgta ggtgtcttac caaatgcaaa tggctgggac aaaccggata tttggatggc
     7861 aaaaacgatt attcctcgac caaattctca ataataaaaa gaggttgtga cagaagtaat
     7921 cagcttcaag aaataagaag gaatttacga aaattacttc ttgaattttt gtgaaattcc
     7981 aacttatttc cgaagaagct acttttggaa caccgtgtac cagaggttgt gaaaaagtcg
     8041 cttagtttcg agtagtaaga a
//
LOCUS       DARBET010000245         7413 bp    DNA     linear   BCT 07-NOV-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4027
            SAMEA114569841-rid20112183.guided.104, whole genome shotgun
            sequence.
ACCESSION   DARBET010000245 DARBET010000000
VERSION     DARBET010000245.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA114569841
            Sequence Read Archive: ERR12203666
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 7413)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7413)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001972618.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001972618.1
            Genome Coverage       :: 132x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/07/2023 13:18:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,041
            CDSs (total)                      :: 2,975
            Genes (coding)                    :: 2,908
            CDSs (with protein)               :: 2,908
            Genes (RNA)                       :: 66
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 67
            CDSs (without protein)            :: 67
            Pseudo Genes (ambiguous residues) :: 0 of 67
            Pseudo Genes (frameshifted)       :: 26 of 67
            Pseudo Genes (incomplete)         :: 28 of 67
            Pseudo Genes (internal stop)      :: 20 of 67
            Pseudo Genes (multiple problems)  :: 6 of 67
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7413
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA114569841-rid20112183.guided.104"
                     /isolate="VRE4027"
                     /db_xref="taxon:1352"
                     /country="Denmark"
     gene            177..1445
                     /locus_tag="SCJ86_003018"
     CDS             177..1445
                     /locus_tag="SCJ86_003018"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 73 protein"
                     /protein_id="HEG1170484.1"
                     /translation="MKAKNLLFSVILLGNLAVANTTVLAEESTETVATQTSSSSETGI
                     ENDTESTLQSATVSENTISTEEPTTETTEQTQTEDSTSAEAQNNPENAEEITNQLTDG
                     QTLTHTHQADLSEFENYTGYAFYARSSANSQQAFIDSIASTAQSLASANDLYASVMIA
                     QAIVESGWGNSTLASAPNYNLFGIKGSYNGQSVTMPTSEYVNGQWITVNAAFRKYPSY
                     KESLQDNVTVLKTTSFQPGVYYYSGAWKSNTNSYKDATAWLTGRYATAPNYGSTLNNV
                     IETYNLTQYDTAPTSTSPMYRLYNRHTGEHLYTLNAGEKNYLPTVGWEYEGIAWQAPN
                     SGQPVYRLYNPYSGDHHYTMAQSEINSLTKIGWRYEGLSFYSGGSKPIYRLFNPNEKT
                     GTHHYTLSAKERDWLTPMGWRYEGIGFYGY"
     gene            1932..2396
                     /locus_tag="SCJ86_003019"
     CDS             1932..2396
                     /locus_tag="SCJ86_003019"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373333.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HEG1170485.1"
                     /translation="MSLEEISHLLYQIKLADQKIVQIFEENIGISLTRYEIMMRLKEE
                     NPLLQSSLQEKLQIDQGAVTRHLKVLEANGYVSRQRNPQNNREVYVALTEKAQKKIKH
                     CEMDHKDTGSVLSPAFSDVEMKQFLTLLDKLNTQLCNITTETKEVNNNGTKK"
     gene            2380..3009
                     /locus_tag="SCJ86_003020"
     CDS             2380..3009
                     /locus_tag="SCJ86_003020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287459.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitroreductase family protein"
                     /protein_id="HEG1170486.1"
                     /translation="MEQKNDFSTIMTGRRSVRVYDETYKIPHEEMLEMIKEATTAPSS
                     VNMKPWRFVVAESEEAKEILRPLIRFNTRQNDTSSAMVMIFGDMQCYEYGEEIYNQAY
                     ESGKMPKEVRDQQLAAIIPYYKSFSREEMNDVVKVDSSLAAMQFMLVARDHGYETNPI
                     GGFEADQLAEAFGLDKDRYVPVIILSIGKAVEEGYESVRLAPEKITMFE"
     gene            3363..4841
                     /gene="msr(C)"
                     /locus_tag="SCJ86_003021"
     CDS             3363..4841
                     /gene="msr(C)"
                     /locus_tag="SCJ86_003021"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314314.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F type ribosomal protection protein Msr(C)"
                     /protein_id="HEG1170487.1"
                     /translation="MENLAVNITNLQVSFGNQLELSIDSLRVYQQDRIGIIGENGVGK
                     STLLKLIAGELFPDHGKIQTEITFNYLPQLTYLAEAKDLNLELASHFQLRLEETSERK
                     WSGGEERKIELIRLLSSYEQGMLLDEPTTHLDRKSIDRLIEELRYYYGTLVFVSHDRY
                     FLDELASKIWEVKDGEIREFSGNYSAYLTQKELEKKTQLREAESIMKEKKRLEKSIQE
                     KKKQAEKLEKVSSKKKKQQIRLDRLSSSKQKDSVQKAIQKNAKTLERRLQKIGETTKP
                     QQMKQIRFPVPKSLELHSRYPIMGQNVQLERSGRTLLVNGDFQFSLGKKIAIVGENGS
                     GKTTLLEHIRKQGEGILLSPKVSFQVYQQKGYQMTSEESIIRFVMRQTEFSESLVRSL
                     LNHLGFAQETLTKPLCTLSGGEATRLTIALLFTKPSNVLLLDEPTNFIDMATIEALEK
                     LMQIYPGTILFTSHDSYFVERTADEVYEIKGQKIKKVLTRNF"
     gene            4888..5334
                     /locus_tag="SCJ86_003022"
     CDS             4888..5334
                     /locus_tag="SCJ86_003022"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328790.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2798 domain-containing protein"
                     /protein_id="HEG1170488.1"
                     /translation="MPTNKKEGIIFTTMMCFLMVLGMSAYNLWLHQDLHLSSLLIGLV
                     PGFVVAFILDVFIIGVLAKKIAFKLPVNPESKVQMILTISCLMVLGMVTCMSLFGVIM
                     EQGIPDQLFSAYMNAWKMNVIAALPLQLIIVGPLSRSVLKAIQSNE"
     gene            complement(5533..6048)
                     /locus_tag="SCJ86_003023"
     CDS             complement(5533..6048)
                     /locus_tag="SCJ86_003023"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629798.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PadR family transcriptional regulator"
                     /protein_id="HEG1170489.1"
                     /translation="MNTLAYILLCVLLSKPRSGYELKQLITIFWEAHHSQIYTTLAKL
                     DQQGYVTVLDKDEHSQKKIYHLTDEGRALVEEWIKEETPAPTQKDEFLAKIYAFSTLD
                     KTTATGLLFTREQQLNKIMQKNQKKLNDLSLDKKQEFGRYVVIQRRIMLCQQELQWCQ
                     QVREQMQQFFE"
     gene            6165..7367
                     /locus_tag="SCJ86_003024"
     CDS             6165..7367
                     /locus_tag="SCJ86_003024"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEG1170490.1"
                     /translation="MEILENQIDIQEQTNEETKGKSSALVVAATCMIAFMGVGLVDPI
                     LKTIAAQLNATPAETTLLFTSYMLVTGVVMLFTGFLSSRIGLKKTLMTGLFIIVVFAG
                     LGGLSGNIGMLIGLRAGWGVGNALFVSTALATIVSILVGNTEKAIMMYEAALGCGMAV
                     GPLVGGVLGSGSWRYPFFGVAFLMFCGFIALAVLLPETEKPKRKVGLWEGVKALSNHK
                     LRSVGLIALLYNFGFFTLLAYAPFLLIGYSELEVGFVFFGWGVLLAIASIFVAPALER
                     KFTTYRTILAAFVLFIICLLLLGAGTSMPVLVPISIVLAGFFQGIINTLLTTIAMEIP
                     GLQRNVASSSYSFVRFFGGALAPFIAGVIGEHIGESYAFYFAGAIVLISVCCIIYHRN
                     YFATNEEE"
BASE COUNT         2350 a         1271 c         1535 g         2257 t
ORIGIN      
        1 ccatcaattt aaggcgtgaa tggagaagaa tattcttcat ttacgtcttt ttatgcagaa
       61 aaagcaaatt tattaaaaag aaacaaatgt tatttaaata tggacattga cttgctttta
      121 cttttttagt tacactgtta tttaggaaaa gtttcataat tcaaaggaga aaaaagatga
      181 aagcaaagaa tttattattt agcgttattc ttttaggaaa tctagcagtt gccaacacaa
      241 cagtgctagc tgaagaatcg acagaaactg tcgcaactca gacatcgagt tcttcagaaa
      301 caggaattga aaacgataca gaaagtactt tgcagtcagc tactgtctca gaaaatacaa
      361 tttcaaccga agaaccaaca actgaaacaa cagaacaaac acaaaccgaa gacagtacat
      421 cagcagaagc tcagaacaat ccagagaatg cagaagaaat cacgaaccaa ttgacagatg
      481 ggcaaacatt gacacatacc catcaagcag atttgtctga attcgaaaac tatactggct
      541 atgcttttta tgctcgctca tctgctaatt ctcaacaagc gttcattgat agtattgcct
      601 caactgcaca aagtttggca agtgccaatg acttatatgc atcagtcatg attgctcaag
      661 ccatcgttga aagtggctgg gggaacagta ctttggcttc tgcccctaat tacaatctat
      721 ttgggattaa aggaagctat aatggccaat ctgttacgat gcctacttcg gaatatgtga
      781 atgggcaatg gattacggtg aatgccgctt ttagaaaata tccttcgtac aaagaatcat
      841 tgcaagataa cgtaacggta ttgaagacga cttcttttca gccaggcgtt tactactatt
      901 ccggtgcatg gaaaagcaat acgaactctt acaaagatgc tacggcttgg ctaacaggtc
      961 gttatgccac tgcgcctaat tatggttcta cattgaacaa tgtaatcgaa acctataatc
     1021 tgacacagta tgatacagca ccaacttcaa cttctccgat gtatcgtctg tataaccgtc
     1081 atacaggaga acatttatat acattgaatg ccggagaaaa aaattatctg ccaactgtag
     1141 gctgggaata tgaagggatt gcttggcaag caccaaacag cggacaacct gtctaccgtt
     1201 tatacaatcc ttacagtgga gaccatcatt acacaatggc acaatctgag atcaactctt
     1261 taacaaaaat cggttggcgt tatgaaggtc taagttttta ttctggtggt tccaagccaa
     1321 tttatcgact atttaatcca aatgaaaaaa ctggtaccca tcactatacc ttgagtgcta
     1381 aggaaagaga ttggctaaca ccgatgggct ggagatatga aggaatagga ttttatggtt
     1441 attaaaaaga ggtagtgaaa aagctgccct gcatcaagta gtaagaacag ccaaacaaaa
     1501 acagattttc atgttttttg tttggttgaa cttattaccg cagatgcaag tttttgaaca
     1561 ccgtttactc aaaaaaggtt gtgacaacat cttgtcataa cttttttatt actgtttaag
     1621 catactgatc caatttctaa tattcgacgg gattcttgca aaaggcgatt tcggaaattt
     1681 ttggcggtgt aaaattatct actgcagaag agctatcttc tatagtttga taagaatcaa
     1741 taatcttggt tttagcgttg tgctttcttt atcatttttt ccgttcattc tacaaaaaat
     1801 ttcttttctc tatacttttc tattgagagc gtttttttta ttacacagct gattctttga
     1861 cttttttctt tttttattct attattgaca agtcaatcgt tgacgagtca attattagaa
     1921 aggaggaaat tatgtcttta gaagagatta gtcacctttt atatcaaatc aaattagccg
     1981 atcaaaaaat cgtccagatc tttgaagaaa atatcggtat aagtcttact agatacgaga
     2041 tcatgatgag actgaaagaa gaaaatccat tgctacaaag cagtttgcaa gaaaagttgc
     2101 agattgatca aggagcagtt actcgtcatc tgaaggtatt ggaagcaaat ggctatgttt
     2161 cgcgtcagag aaatcctcag aacaaccggg aagtgtatgt agcgctaaca gaaaaagccc
     2221 agaagaaaat caagcattgt gaaatggacc ataaagatac aggttccgtg ctatccccgg
     2281 ctttcagcga tgtagaaatg aaacaatttc taacactttt agataaattg aatacccaac
     2341 tttgcaatat aacaacagaa acgaaagagg ttaataataa tggaacaaaa aaatgatttt
     2401 tcaacaatta tgactggtag aagatccgtt cgcgtatatg atgaaacata caaaatccca
     2461 cacgaagaaa tgctggaaat gatcaaagaa gcgacgactg caccttcttc tgtcaatatg
     2521 aaaccatggc gctttgtggt cgctgaaagc gaggaagcaa aagaaatctt gcgtcctttg
     2581 atccgtttca atacgagaca aaatgatact tcttctgcaa tggtcatgat ttttggcgat
     2641 atgcaatgtt acgaatatgg cgaagaaatc tacaaccaag cttacgaatc agggaagatg
     2701 ccaaaagaag tcagagatca acaattagct gcgatcattc cttattacaa gagtttttca
     2761 agagaagaaa tgaacgatgt agtcaaagtc gattctagct tagcggctat gcaattcatg
     2821 ttagttgcgc gtgatcatgg ctatgaaaca aatccaattg gtggattcga agcagatcag
     2881 ttggcagagg catttggttt ggataaagac cgttatgttc cagttattat tttatcaata
     2941 ggaaaagcgg tagaagaagg ttacgagtct gttcgtttag caccagaaaa gatcactatg
     3001 ttcgaataaa aggttttcag ttactattca gtagctattg ctttttttga atcgtgtgtg
     3061 gtaacctaat aaaaaaggag gtcggaaaaa tgactgcatc gatgaaatta cgtttcgaac
     3121 ttttgaataa caactaaata aattcaaaag acatgtataa acaacaattt catggatgga
     3181 gtatgtccat tttttcgaac gacagtagaa taggtgaaag ctatgacaga tttggtgatt
     3241 tagtcatagt tatttcgttc agagtaggaa aagatgtgat gcagtttttt tgcaaacaga
     3301 tttttctgct cttttttagt atcgataatt gttgttttac atgaaatagg agtgtaaaac
     3361 atatggaaaa tttagcagta aatataacaa atctacaagt cagctttggc aaccagctag
     3421 aactatctat tgattctctt cgtgtctatc agcaagatcg tatagggatc ataggagaaa
     3481 atggtgtcgg taaatcgact ttgctcaaac taatagccgg tgaacttttt cccgatcatg
     3541 ggaaaatcca aacagagatc accttcaact acctgcctca attaacctat ctcgctgaag
     3601 caaaggacct aaatttggaa ttagccagtc atttccagtt aagactggaa gaaacttcag
     3661 agcggaaatg gagcggaggg gaagaacgaa agatcgagtt gatacgtctt ctttcttctt
     3721 atgaacaagg gatgcttcta gacgagccaa caacccatct agatagaaaa agtattgatc
     3781 gactgattga agagcttcgt tattattatg gtacgctggt ttttgttagt catgatcgct
     3841 attttctaga tgagctagca tcgaaaatct gggaagtaaa agacggagaa atccgagagt
     3901 tttcggggaa ttatagtgcc tatctcactc aaaaggaatt ggagaaaaag actcagctac
     3961 gagaagcaga gtcgatcatg aaagagaaaa aacgattgga aaaatcgatc caagaaaaga
     4021 aaaaacaagc ggaaaagttg gaaaaagtgt ccagtaaaaa gaaaaagcaa caaatcagac
     4081 tggatcggtt gtcttcctct aaacaaaaag acagtgtaca aaaagccatc caaaagaatg
     4141 cgaaaacatt agagagaaga ctccaaaaaa taggagaaac aaccaagccg cagcagatga
     4201 aacaaatccg ttttccagta ccaaaatctc ttgaactcca cagccgctat cccatcatgg
     4261 gacaaaatgt ccaattggaa agaagtggga gaacattact ggtaaatggt gattttcagt
     4321 tttctttagg taaaaaaatc gcgattgtcg gtgaaaatgg ttcaggtaag acaaccttat
     4381 tagaacatat ccgcaaacaa ggagaaggaa tccttctctc tccgaaagta agctttcaag
     4441 tatatcagca aaagggttat caaatgacat ctgaagaatc catcattcgt tttgtcatga
     4501 gacaaacaga gttttcagaa tcgcttgtcc gtagtttgct gaatcactta gggtttgctc
     4561 aggaaacttt gacgaaaccg ttatgtacat taagtggggg agaagcgacc cgtctgacga
     4621 ttgctttgct ttttactaag ccaagtaatg tgttgctgtt agatgaaccg actaatttta
     4681 ttgatatggc aacgatcgaa gctttagaga agctaatgca aatatatccg ggaacgattt
     4741 tgtttacttc tcatgattct tactttgtcg agcgtacggc tgatgaagtt tatgaaataa
     4801 aagggcagaa aataaaaaaa gtacttacga gaaattttta aatgtgctac gatgattttc
     4861 gtgacaaatt taaaggagtg gtacagattg cctacaaata aaaaagaagg aataattttt
     4921 acaacaatga tgtgtttttt gatggtcttg gggatgagcg cgtataattt atggctgcat
     4981 caagatcttc atttatcaag tttgttgatt ggtctagtgc ctggattcgt ggtggctttc
     5041 attttggatg tcttcatcat cggtgtgctg gcgaaaaaaa ttgcctttaa actaccagta
     5101 aatccagaaa gcaaggtcca aatgatctta actatttcat gcttgatggt tttaggaatg
     5161 gtgacatgta tgtctttatt cggtgtgatc atggaacaag gaatacctga tcaattattc
     5221 tctgcttata tgaatgcttg gaaaatgaat gtgattgccg ctttaccgtt gcagttgatc
     5281 atcgttggcc cgctttcaag aagtgtgcta aaagcaattc aaagtaatga atgatggaac
     5341 actgttattt gaaaaaaggc cttggcatgc tttttgtcat agtctcttga atgcagttct
     5401 agaaaaatag tgatccgaac gataggtttt ggcctgtcgt tcggatcgct ttttcttttc
     5461 caactgtatt gctcttttat tgaagataat ttgctcttca atagatattt ctagaaaggt
     5521 tctgtttatc ccttactcaa aaaactgctg catttgttct cttacttgct gacaccattg
     5581 gagttcctgc tgacaaagca taattctgcg ctggatcaca acataacgtc caaattcttg
     5641 tttcttgtct agcgagaggt cattcaattt tttttgattt ttttgcatga ttttgttcag
     5701 ctgttgttct ctagtgaata gcagtccagt agctgttgtt ttatcgagcg tcgaaaaagc
     5761 atagattttt gctagaaatt catctttttg cgtaggagcg ggagtttcct ctttgatcca
     5821 ttcctctaca agagcacgtc cttcatcagt caagtggtag atcttttttt gagagtgctc
     5881 gtctttgtcc aaaacggtca cataaccctg ctggtctaac ttggcaagcg ttgtatagat
     5941 ctgactgtga tgggcttccc aaaaaattgt gatcagctgc ttcaattcat acccagaacg
     6001 tggtttggat aaaagcacgc ataataatat ataggctaaa gtattcataa aaatcctacc
     6061 tctcgtctta ttttctttat tatatcacag taaagtttcc acgttttaca tgtgcatatt
     6121 gacatgtaaa ataaatcatg tatgatgcta atagagagaa agggatggaa atattggaga
     6181 atcaaataga tatacaagaa caaacaaatg aagaaacaaa ggggaaaagt tcagcgctag
     6241 ttgtagcagc tacctgtatg atcgctttta tgggagtggg tctagtagac ccaatcttaa
     6301 aaacgattgc tgcacagtta aacgctacgc cagctgaaac gaccctgtta tttactagtt
     6361 acatgttagt cactggtgtg gtgatgctat ttacaggttt cttatctagt cggatcggtt
     6421 tgaaaaaaac attgatgaca ggattgttta tcatcgttgt attcgctggt cttggcggat
     6481 tatctgggaa tatcggaatg ctgattgggt tacgtgccgg ctggggtgtc gggaatgctt
     6541 tatttgtctc tactgcttta gcaacgatcg tcagtatttt agttggtaac acagaaaaag
     6601 caatcatgat gtatgaagca gcacttggct gcggaatggc tgttggccct ctagtcggtg
     6661 gggtcttggg cagtggttca tggcgttatc cattttttgg ggtagcattt ttgatgtttt
     6721 gcggttttat agccctagcg gtacttttac ctgaaactga gaaaccaaag agaaaagtag
     6781 gcctttggga aggtgtcaaa gcgttaagca atcataaact tcgttcagtt ggactaatcg
     6841 cattgttata taatttcgga ttctttactt tacttgcgta tgcgccattt ttattgattg
     6901 gctattctga attagaagtc ggttttgtat tttttggctg gggtgtgctt ctggctattg
     6961 cttcgatttt cgttgcaccg gctcttgagc ggaagtttac gacttatcgg acaattttag
     7021 cagcatttgt cttattcatt atttgtttgc tgttattagg agctggaact tctatgccag
     7081 tccttgtacc gatcagcatt gtgttagcag gatttttcca aggaattatc aatacactgt
     7141 tgacgacgat tgctatggaa ataccaggtc tgcaacgaaa cgttgcttcg tccagctata
     7201 gttttgtccg ctttttcggt ggagctttag caccatttat tgcaggtgtg attggtgaac
     7261 atattggtga aagctatgcg ttttattttg caggtgccat tgttcttatc agtgtatgtt
     7321 gtatcattta tcaccgtaat tattttgcta caaatgaaga agaataagaa atcagaggtt
     7381 gtgaaaaagc tgtcctgcat caagtcataa gaa
//
LOCUS       DARBET010000246         5613 bp    DNA     linear   BCT 07-NOV-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4027
            SAMEA114569841-rid20112183.guided.124, whole genome shotgun
            sequence.
ACCESSION   DARBET010000246 DARBET010000000
VERSION     DARBET010000246.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA114569841
            Sequence Read Archive: ERR12203666
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 5613)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5613)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001972618.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001972618.1
            Genome Coverage       :: 132x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/07/2023 13:18:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,041
            CDSs (total)                      :: 2,975
            Genes (coding)                    :: 2,908
            CDSs (with protein)               :: 2,908
            Genes (RNA)                       :: 66
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 67
            CDSs (without protein)            :: 67
            Pseudo Genes (ambiguous residues) :: 0 of 67
            Pseudo Genes (frameshifted)       :: 26 of 67
            Pseudo Genes (incomplete)         :: 28 of 67
            Pseudo Genes (internal stop)      :: 20 of 67
            Pseudo Genes (multiple problems)  :: 6 of 67
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5613
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA114569841-rid20112183.guided.124"
                     /isolate="VRE4027"
                     /db_xref="taxon:1352"
                     /country="Denmark"
     gene            complement(40..1026)
                     /locus_tag="SCJ86_003025"
                     /pseudo
     CDS             complement(40..1026)
                     /locus_tag="SCJ86_003025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_032495458.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS256-like element IS1542 family transposase"
     gene            1178..1873
                     /gene="vanR"
                     /locus_tag="SCJ86_003026"
     CDS             1178..1873
                     /gene="vanR"
                     /locus_tag="SCJ86_003026"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001280781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VanA-type vancomycin resistance DNA-binding
                     response regulator VanR"
                     /protein_id="HEG1170491.1"
                     /translation="MSDKILIVDDEHEIADLVELYLKNENYTVFKYYTAKEALECIDK
                     SEIDLAILDIMLPGTSGLTICQKIRDKHTYPIIMLTGKDTEVDKITGLTIGADDYITK
                     PFRPLELIARVKAQLRRYKKFSGVKEQNENVIVHSGLVINVNTHECYLNEKQLSLTPT
                     EFSILRILCENKGNVVSSELLFHEIWGDEYFSKSNNTITVHIRHLREKMNDTIDNPKY
                     IKTVWGVGYKIEK"
     gene            1851..3005
                     /locus_tag="SCJ86_003027"
     CDS             1851..3005
                     /locus_tag="SCJ86_003027"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305818.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VanA-type vancomycin resistance histidine kinase
                     VanS"
                     /protein_id="HEG1170492.1"
                     /translation="MVIKLKNKKNDYSKLERKLYMYIVAIVVVAIVFVLYIRSMIRGK
                     LGDWILSILENKYDLNHLDAMKLYQYSIRNNIDIFIYVAIVISILILCRVMLSKFAKY
                     FDEINTGIDVLIQNEDKQIELSAEMDVMEQKLNTLKRTLEKREQDAKLAEQRKNDVVM
                     YLAHDIKTPLTSIIGYLSLLDEAPDMPVDQKAKYVHITLDKAYRLEQLIDEFFEITRY
                     NLQTITLTKTHIDLYYMLVQMTDEFYPQLSAHGKQAVIHAPEDLTVSGDPDKLARVFN
                     NILKNAAAYSEDNSIIDITAGLSGDVVSIEFKNTGSIPKDKLAAIFEKFYRLDNARSS
                     DTGGAGLGLAIAKEIIVQHGGQIYAESNDNYTTFRVELPAMPDLVDKRRS"
     gene            3220..4188
                     /locus_tag="SCJ86_003028"
     CDS             3220..4188
                     /locus_tag="SCJ86_003028"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001059542.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-lactate dehydrogenase VanH-A"
                     /protein_id="HEG1170493.1"
                     /translation="MNNIGITVYGCEQDEADAFHALSPRFGVMATIINANVSESNAKS
                     APFNQCISVGHKSEISASILLALKRAGVKYISTRSIGCNHIDTTAAKRMGITVDNVAY
                     SPDSVADYTMMLILMAVRNVKSIVRSVEKHDFRLDSDRGKVLSDMTVGVVGTGQIGKA
                     VIERLRGFGCKVLAYSRSRSIEVNYVPFDELLQNSDIVTLHVPLNTDTHYIISHEQIQ
                     RMKQGAFLINTGRGPLVDTYELVKALENGKLGGAALDVLEGEEEFFYSDCTQKPIDNQ
                     FLLKLQRMPNVIITPHTAYYTEQALRDTVEKTIKNCLDFERRQEHE"
     gene            4181..5212
                     /gene="vanA"
                     /locus_tag="SCJ86_003029"
     CDS             4181..5212
                     /gene="vanA"
                     /locus_tag="SCJ86_003029"
                     /EC_number="6.1.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001079845.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanine--(R)-lactate ligase VanA"
                     /protein_id="HEG1170494.1"
                     /translation="MNRIKVAILFGGCSEEHDVSVKSAIEIAANINKEKYEPLYIGIT
                     KSGVWKMCEKPCAEWENDNCYSAVLSPDKKMHGLLVKKNHEYEINHVDVAFSALHGKS
                     GEDGSIQGLFELSGIPFVGCDIQSSAICMDKSLTYIVAKNAGIATPAFWVINKDDRPV
                     AATFTYPVFVKPARSGSSFGVKKVNSADELDYAIESARQYDSKILIEQAVSGCEVGCA
                     VLGNSAALVVGEVDQIRLQYGIFRIHQEVEPEKGSENAVITVPADLSAEERGRIQETA
                     KKIYKALGCRGLARVDMFLQDNGRIVLNEVNTLPGFTSYSRYPRMMAAAGIALPELID
                     RLIVLALKG"
     gene            5218..5583
                     /locus_tag="SCJ86_003030"
                     /pseudo
     CDS             5218..5583
                     /locus_tag="SCJ86_003030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000402347.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="D-Ala-D-Ala dipeptidase VanX"
BASE COUNT         1697 a         1091 c         1262 g         1563 t
ORIGIN      
        1 ggattttatt ctttatttaa aactttgcaa cagaacccta tttccgataa ccgtgtattg
       61 aaaggcatct tcgaagccat tatccaatat ttcgcaggct tttgtatatt taggctggtc
      121 gatatattca ccgactaagg cattcttagc tgttcgagcg agttcaatat ccgtaaactt
      181 aaagatcgct tttactgctt ctctaaacgg ttttgaattc ttttttggaa tggaactgaa
      241 gatgtttctt aaaaaatgga cctggcatct ctgccaactt gcgttggtaa atgacttacg
      301 aatcgcagac actaggcctt tatgggcatc agaaatgatg agttctgtcc cctttaggcc
      361 gcgttctttt aagtattcaa agaagatgga ccatgtgtca tcactttctt cattttgaat
      421 catgaagcca atgatttcac ggtcgccacc ttctgttatc ccgatcgcaa tatggcagct
      481 tttagaaagc actcgatggt cctcacggac ttttatgtag agaacatcag tcatcagata
      541 aggataattc gtacctgaga gtgaacggtt ctgccattcg ttgaccatcg ggtctaactg
      601 ctcagtcagg ctagaaacaa aagatttcga tacagatttt ccacatagct cttcaacaat
      661 ctttgaaact ttacgtgtcg aaacgcccga aacatacatc tcaagcattg aagcgagcag
      721 tgccttttca tttcgctgat aacgctcaaa caccgtcggt gaaaattcac catcacgtgt
      781 tctaggcact tttaattcga gtgtacccac acgagtcgta aagtctcgct cataatagcc
      841 atttctttga ctctgacgac tttctgaacg ttcatagtca tcggcttgaa tatattctgt
      901 tcgttgattt tccatcaatt ggttgaaaac agtggttaaa atatttttag aaacatcatc
      961 ttttaccgaa tgttcaataa tactttgaac ctcttcgttg ttcagtgtaa aatgtacttg
     1021 ggtcatgtaa cgtcctcctg ggtatgtttt tgtggttaaa aacattgtac cgtaaaaggg
     1081 ctgttacatg gccttttatc ttttacacaa ttatatggac tttatcctat aatctgataa
     1141 attattaagg gcaaacttat gtgaaagggt gataactatg agcgataaaa tacttattgt
     1201 ggatgatgaa catgaaattg ccgatttggt tgaattatac ttaaaaaacg agaattatac
     1261 ggttttcaaa tactataccg ccaaagaagc attggaatgt atagacaagt ctgagattga
     1321 ccttgccata ttggacatca tgcttcccgg cacaagcggc cttactatct gtcaaaaaat
     1381 aagggacaag cacacctatc cgattatcat gctgaccggg aaagatacag aggtagataa
     1441 aattacaggg ttaacaatcg gcgcggatga ttatataacg aagccctttc gcccactgga
     1501 gttaattgct cgggtaaagg cccagttgcg ccgatacaaa aaattcagtg gagtaaagga
     1561 gcagaacgaa aatgttatcg tccactccgg ccttgtcatt aatgttaaca cccatgagtg
     1621 ttatctgaac gagaagcagt tatcccttac tcccaccgag ttttcaatac tgcgaatcct
     1681 ctgtgaaaac aaggggaatg tggttagctc cgagctgcta tttcatgaga tatggggcga
     1741 cgaatatttc agcaagagca acaacaccat caccgtgcat atccggcatt tgcgcgaaaa
     1801 aatgaacgac accattgata atccgaaata tataaaaacg gtatgggggg ttggttataa
     1861 aattgaaaaa taaaaaaaac gactattcca aactagaacg aaaactttac atgtatatcg
     1921 ttgcaattgt tgtggtagca attgtattcg tgttgtatat tcgttcaatg atccgaggga
     1981 aacttgggga ttggatctta agtattttgg aaaacaaata tgacttaaat cacctggacg
     2041 cgatgaaatt atatcaatat tccatacgga acaatataga tatctttatt tatgtggcga
     2101 ttgtcattag tattcttatt ctatgtcgcg tcatgctttc aaaattcgca aaatactttg
     2161 acgagataaa taccggcatt gatgtactta ttcagaacga agataaacaa attgagcttt
     2221 ctgcggaaat ggatgttatg gaacaaaagc tcaacacatt aaaacggact ctggaaaagc
     2281 gagagcagga tgcaaagctg gccgaacaaa gaaaaaatga cgttgttatg tacttggcgc
     2341 acgatattaa aacgcccctt acatccatta tcggttattt gagcctgctt gacgaggctc
     2401 cagacatgcc ggtagatcaa aaggcaaagt atgtgcatat cacgttggac aaagcgtatc
     2461 gactcgaaca gctaatcgac gagttttttg agattacacg gtataaccta caaacgataa
     2521 cgctaacaaa aacgcacata gacctatact atatgctggt gcagatgacc gatgaatttt
     2581 atcctcagct ttccgcacat ggaaaacagg cggttattca cgcccccgag gatctgaccg
     2641 tgtccggcga ccctgataaa ctcgcgagag tctttaacaa cattttgaaa aacgccgctg
     2701 catacagtga ggataacagc atcattgaca ttaccgcggg cctctccggg gatgtggtgt
     2761 caatcgaatt caagaacact ggaagcatcc caaaagataa gctagctgcc atatttgaaa
     2821 agttctatag gctggacaat gctcgttctt ccgatacggg tggcgcggga cttggattgg
     2881 cgattgcaaa agaaattatt gttcagcatg gagggcagat ttacgcggaa agcaatgata
     2941 actatacgac gtttagggta gagcttccag cgatgccaga cttggttgat aaaaggaggt
     3001 cctaagagat gtatataatt ttttaggaaa atctcaaggt tatctttact ttttcttagg
     3061 aaattaacaa tttaatatta agaaacggct cgttcttaca cggtagactt aataccgtaa
     3121 gaacgagccg ttttcgttct tcagagaaag atttgacaag attaccattg gcatccccgt
     3181 tttatttggt gcctttcaca gaaagggttg gtcttaatta tgaataacat cggcattact
     3241 gtttatggat gtgagcagga tgaggcagat gcattccatg ctctttcgcc tcgctttggc
     3301 gttatggcaa cgataattaa cgccaacgtg tcggaatcca acgccaaatc cgcgcctttc
     3361 aatcaatgta tcagtgtggg acataaatca gagatttccg cctctattct tcttgcgctg
     3421 aagagagccg gtgtgaaata tatttctacc cgaagcatcg gctgcaatca tatagataca
     3481 actgctgcta agagaatggg catcactgtc gacaatgtgg cgtactcgcc ggatagcgtt
     3541 gccgattata ctatgatgct aattcttatg gcagtacgca acgtaaaatc gattgtgcgc
     3601 tctgtggaaa aacatgattt caggttggac agcgaccgtg gcaaggtact cagcgacatg
     3661 acagttggtg tggtgggaac gggccagata ggcaaagcgg ttattgagcg gctgcgagga
     3721 tttggatgta aagtgttggc ttatagtcgc agccgaagta tagaggtaaa ctatgtaccg
     3781 tttgatgagt tgctgcaaaa tagcgatatc gttacgcttc atgtgccgct caatacggat
     3841 acgcactata ttatcagcca cgaacaaata cagagaatga agcaaggagc atttcttatc
     3901 aatactgggc gcggtccact tgtagatacc tatgagttgg ttaaagcatt agaaaacggg
     3961 aaactgggcg gtgccgcatt ggatgtattg gaaggagagg aagagttttt ctactctgat
     4021 tgcacccaaa aaccaattga taatcaattt ttacttaaac ttcaaagaat gcctaacgtg
     4081 ataatcacac cgcatacggc ctattatacc gagcaagcgt tgcgtgatac cgttgaaaaa
     4141 accattaaaa actgtttgga ttttgaaagg agacaggagc atgaatagaa taaaagttgc
     4201 aatactgttt gggggttgct cagaggagca tgacgtatcg gtaaaatctg caatagagat
     4261 agccgctaac attaataaag aaaaatacga gccgttatac attggaatta cgaaatctgg
     4321 tgtatggaaa atgtgcgaaa aaccttgcgc ggaatgggaa aacgacaatt gctattcagc
     4381 tgtactctcg ccggataaaa aaatgcacgg attacttgtt aaaaagaacc atgaatatga
     4441 aatcaaccat gttgatgtag cattttcagc tttgcatggc aagtcaggtg aagatggatc
     4501 catacaaggt ctgtttgaat tgtccggtat cccttttgta ggctgcgata ttcaaagctc
     4561 agcaatttgt atggacaaat cgttgacata catcgttgcg aaaaatgctg ggatagctac
     4621 tcccgccttt tgggttatta ataaagatga taggccggtg gcagctacgt ttacctatcc
     4681 tgtttttgtt aagccggcgc gttcaggctc atccttcggt gtgaaaaaag tcaatagcgc
     4741 ggacgaattg gactacgcaa ttgaatcggc aagacaatat gacagcaaaa tcttaattga
     4801 gcaggctgtt tcgggctgtg aggtcggttg tgcggtattg ggaaacagtg ccgcgttagt
     4861 tgttggcgag gtggaccaaa tcaggctgca gtacggaatc tttcgtattc atcaggaagt
     4921 cgagccggaa aaaggctctg aaaacgcagt tataaccgtt cccgcagacc tttcagcaga
     4981 ggagcgagga cggatacagg aaacggcaaa aaaaatatat aaagcgctcg gctgtagagg
     5041 tctagcccgt gtggatatgt ttttacaaga taacggccgc attgtactga acgaagtcaa
     5101 tactctgccc ggtttcacgt catacagtcg ttatccccgt atgatggccg ctgcaggtat
     5161 tgcacttccc gaactgattg accgcttgat cgtattagcg ttaaaggggt gataagcatg
     5221 gaaataggat ttactttttt agatgaaata gtacacggtg ttcgttggga cgctaaatat
     5281 gccacttggg ataatttcac cggaaaaccg gttgacggtt atgaagtaaa tcgcattgta
     5341 gggacatacg agttggctga atcgcttttg aaggcaaaag aactggctgc tacccaaggg
     5401 tacggattgc ttctatggga cggttaccgt cctaagcgtg ctgtaaactg ttttatgcaa
     5461 tgggctgcac agccggaaaa taacctgaca aaggaaagtt attatcccaa tattgaccga
     5521 actgagatga tttcaaaagg atacgtggct tcaaaatcaa gccatagccg cggcggttct
     5581 gttgcaaagt tttaaataaa gaataaaatc cct
//
LOCUS       DARBET010000247         5608 bp    DNA     linear   BCT 07-NOV-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4027
            SAMEA114569841-rid20112183.guided.125, whole genome shotgun
            sequence.
ACCESSION   DARBET010000247 DARBET010000000
VERSION     DARBET010000247.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA114569841
            Sequence Read Archive: ERR12203666
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 5608)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5608)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001972618.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001972618.1
            Genome Coverage       :: 132x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/07/2023 13:18:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,041
            CDSs (total)                      :: 2,975
            Genes (coding)                    :: 2,908
            CDSs (with protein)               :: 2,908
            Genes (RNA)                       :: 66
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 67
            CDSs (without protein)            :: 67
            Pseudo Genes (ambiguous residues) :: 0 of 67
            Pseudo Genes (frameshifted)       :: 26 of 67
            Pseudo Genes (incomplete)         :: 28 of 67
            Pseudo Genes (internal stop)      :: 20 of 67
            Pseudo Genes (multiple problems)  :: 6 of 67
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5608
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA114569841-rid20112183.guided.125"
                     /isolate="VRE4027"
                     /db_xref="taxon:1352"
                     /country="Denmark"
     gene            <1..259
                     /locus_tag="SCJ86_003031"
     CDS             <1..259
                     /locus_tag="SCJ86_003031"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HEG1170495.1"
                     /translation="KPLKYQSVLDLVRRIVKRTGIEFTSHMLRHTHATQLIREGWDVA
                     FVQKRLGHAHVQTTLNTYVHLSDQDMKNEFNKYLERKEHKK"
     gene            256..2148
                     /locus_tag="SCJ86_003032"
     CDS             256..2148
                     /locus_tag="SCJ86_003032"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001833801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HEG1170496.1"
                     /translation="MNASSKRKIISQSEISKKIAVMNEEMQGFWANNSWDIRKCPHPS
                     AIELSKNPALRNRWVRFERVKNLWLRTELKYFYFYHLNNGIWNAKTVWIRKGTVINKM
                     LDFLDLKYPSITSITEVPIEKAMTEYRTYLTKRGVRITTTNYKITANQEKTPVKANSY
                     YVTNLKQFMEFYENFYFDGEEWDKDVWDRRNLPLPDDKVNPTQYEYTINFKGFRNTYF
                     KQLVKRYCKLRLNMDSFSYVSDIAQRLKEFFNFLDMKFKQVQRVHQLTRVEIEAYLSE
                     LNMMGIKPSTITGRISILEGLFSTLLRLEWDDVPSKILIYSEDYPKIPRAKPRFIDEF
                     VLEQLNSHLDKLPEYIATMTMIVQECGMRISELCTLKKGCLLEDKDGDFFLKYYQWKM
                     KKEHIVPISKEVALLIKVREDKVSEEFPDSEYLFPRKDGSPLKQETFRGELNKLAYEQ
                     NIVDKSGEIYRFHAHAFRHTVGTRMINNGMPQHIVQKFLGHESPEMTSRYAHIFDETL
                     KNEFTKFQEKLVTNNGDVLDLDEDNEVDDVELQWFKKNINAQVLPNGYCRLPVVAGGC
                     PHANACLDCTHFCTSKQFLPQHEEQLERTEELLAIAKDKQWQRQVETNSRVKERLEQI
                     IGSLTG"
     gene            2155..2532
                     /locus_tag="SCJ86_003033"
     CDS             2155..2532
                     /locus_tag="SCJ86_003033"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000361059.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HEG1170497.1"
                     /translation="MDKQVRNTTEIVRLAKQKSKKTREKVDKAISKFSIEGKVINFNS
                     IAKEANVSKSWLYKEHDIRQRIESLRERQITANVVSKPKKSSRSEEILIKTLKRRVME
                     LEKENKKLQNQIQKLYGDLYNKE"
     gene            2686..3468
                     /locus_tag="SCJ86_003034"
     CDS             2686..3468
                     /locus_tag="SCJ86_003034"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000067268.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminoglycoside nucleotidyltransferase ANT(9)-Ia"
                     /protein_id="HEG1170498.1"
                     /translation="MSNLINGKIPNQAIQTLKIVKDLFGSSIVGVYLFGSAVNGGLRI
                     NSDVDVLVVVNHSLPQLTRKKLTERLMTISGKIGNTDSVRPLEVTVINRSEVVPWQYP
                     PKREFIYGEWLRGEFENGQIQEPSYDPDLAIVLAQARKNSISLFGPDSSSILVSVPLT
                     DIRRAIKDSLPELIEGIKGDERNVILTLARMWQTVTTGEITSKDVAAEWAIPLLPKEH
                     VTLLDIARKGYRGECDDKWEGLYSKVKALVKYMKNSIETSLN"
     gene            complement(3594..4325)
                     /gene="erm(A)"
                     /locus_tag="SCJ86_003035"
     CDS             complement(3594..4325)
                     /gene="erm(A)"
                     /locus_tag="SCJ86_003035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001072197.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (adenine(2058)-N(6))-methyltransferase
                     Erm(A)"
                     /protein_id="HEG1170499.1"
                     /translation="MNQKNPKDTQNFITSKKHVKEILNHTNISKQDNVIEIGSGKGHF
                     TKELVKMSRSVTAIEIDGGLCQVTKEAVNPSENIKVIQTDILKFSFPKHINYKIYGNI
                     PYNISTDIVKRITFESQAKYSYLIVEKGFAKRLQNLQRALGLLLMVEMDIKMLKKVPP
                     LYFHPKPSVDSVLIVLERHQPLISKKDYKKYRSFVYKWVNREYRVLFTKNQFRQALKH
                     ANVTNINKLSKEQFLSIFNSYKLFH"
     gene            complement(4383..4442)
                     /locus_tag="SCJ86_003036"
     CDS             complement(4383..4442)
                     /locus_tag="SCJ86_003036"
                     /inference="COORDINATES: protein motif:HMM:NF033690.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="erythromycin resistance leader peptide"
                     /protein_id="HEG1170500.1"
                     /translation="MGTFSIFVINKVRYQPNQN"
     gene            4834..5496
                     /locus_tag="SCJ86_003037"
     CDS             4834..5496
                     /locus_tag="SCJ86_003037"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016922249.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HEG1170501.1"
                     /translation="MNTIESQFDKVAEDYDFVNELLNDYSFFVSNMSPKKGRALDIGC
                     GSGLLVEKLASYYDEVVGIDISNQMLDLAKSKRQLTNTVYLNMNAEQLNFNEKFDFIV
                     SRTTFHHLDDIASVIQQMKELLNEEGRIVILDNVSEVETPPTYVYKLGAIQEFLPHCF
                     KFGIKNAIRIYNHNTSKSWLEHLASDKYLSEQNYYDLYEKLLPGCQFHKMGWAMGVVW
                     TK"
BASE COUNT         1993 a          798 c         1071 g         1746 t
ORIGIN      
        1 gaaaccacta aagtaccaaa gtgttcttga tctagttaga agaatagtta aaaggactgg
       61 aatagaattt acatcacata tgcttcgcca cactcacgca acgcagctaa ttagggaagg
      121 atgggatgtt gcgttcgttc aaaagagatt aggtcacgca catgttcaga caacgttaaa
      181 tacctatgtt catctttcag atcaggatat gaaaaatgag tttaataaat acctcgagag
      241 aaaggagcat aagaaatgaa tgcttctagt aaaaggaaaa ttattagtca gagtgagatt
      301 agcaaaaaaa tagctgtaat gaatgaagaa atgcaggggt tttgggctaa taatagttgg
      361 gatataagaa aatgtccaca tccttctgcc atagaattaa gtaagaatcc tgctttaagg
      421 aatcgttggg ttcgttttga acgtgttaaa aatctgtggt taagaacaga attgaaatat
      481 ttttattttt accatttaaa caatggaata tggaatgcaa aaactgtctg gattagaaaa
      541 ggaacagtaa ttaataaaat gttagatttc ttagatttaa agtatcctag cattacttca
      601 attactgaag ttcctattga aaaagcaatg acggagtata gaacttattt gacaaaacgg
      661 ggtgttagaa ttaccactac taattataag attactgcta atcaagaaaa aacacctgta
      721 aaagctaatt cctactatgt tactaatcta aaacaattta tggagtttta tgagaacttt
      781 tattttgatg gagaggagtg ggataaagac gtttgggata gacgtaactt acctttgcca
      841 gatgataagg ttaacccaac acaatatgaa tatacaatta actttaaagg gtttcggaat
      901 acatatttta aacaacttgt aaaaagatat tgtaagttga gattgaacat ggatagcttt
      961 tcctatgtaa gtgatattgc ccaaagactt aaagagttct ttaattttct ggacatgaaa
     1021 tttaaacaag ttcagagagt acaccaatta acgagagtgg aaattgaagc atatttaagt
     1081 gaactaaaca tgatgggaat aaaacctagt acaataactg ggaggatctc tatattggaa
     1141 ggactattta gtacccttct taggctagaa tgggatgatg ttccttccaa aatattaatt
     1201 tattctgagg actatccgaa aataccaaga gcaaaaccac gctttataga tgaattcgtc
     1261 ctagagcaat tgaacagtca tcttgataaa ttacccgaat atatagctac gatgactatg
     1321 attgttcaag aatgtggaat gaggataagt gaattgtgca ccttgaaaaa aggctgtcta
     1381 ttagaggaca aagatggaga tttcttttta aagtattatc aatggaaaat gaaaaaggag
     1441 catatagttc caatatctaa agaggtagct ttacttatta aagttcggga agataaagtt
     1501 tcagaggaat ttccagatag tgaatacctc tttccaagaa aagatggatc gccattaaaa
     1561 caagaaacat ttagaggtga gttaaataaa ttagcttatg agcaaaatat agtggataaa
     1621 tcaggtgaga tttatagatt ccatgcccat gcctttcgcc atacagtagg aacaagaatg
     1681 attaacaacg ggatgcccca gcatattgtg cagaaatttt tggggcatga aagcccagaa
     1741 atgacaagca gatacgctca tatctttgat gaaactctaa aaaatgaatt tactaaattt
     1801 caggaaaaac tggttaccaa taatggagat gtgcttgatc tagatgaaga taatgaagtc
     1861 gatgatgtag agcttcaatg gttcaagaaa aatataaatg cacaagtgct tccaaatggt
     1921 tattgtagat tgccagtagt agcaggtggt tgtccacatg cgaatgcatg cttagattgc
     1981 actcacttct gtaccagtaa gcaattctta ccacagcacg aagaacagtt agagcgtaca
     2041 gaagagttat tagccatagc taaggataaa caatggcaaa gacaagtaga gactaatagc
     2101 cgtgttaaag agcgtttaga acaaatcatt ggaagtttga cggggtaatt atcaatggat
     2161 aaacaagtta gaaatacaac agaaattgta cgtttggcga agcagaaatc aaaaaagaca
     2221 agggaaaaag tagacaaagc gatttctaaa ttttcgattg aaggtaaagt tattaatttt
     2281 aattcaatag caaaggaagc taatgtttct aaatcatggc tttataagga acacgatatt
     2341 aggcaaagaa tcgaatccct tcgtgagcgt caaataacag caaatgtagt ctcaaaaccc
     2401 aagaaaagtt ctcgttcgga ggaaatcctt attaaaacct taaaaagaag agtaatggaa
     2461 ttagaaaaag aaaataaaaa attacagaac caaattcaaa aattatatgg agatctgtat
     2521 aataaagaat aattattaat ctgtagacaa attgtgaaag gatgtactta aacgctaacg
     2581 gtcagcttta ttgaacagtc atttaggtat atgtccaatc tagggtaagt aaattgagta
     2641 tcaatataaa ctttatatga acataatcag ttacgaggtg aaatcatgag caatttgatt
     2701 aacggaaaaa taccaaatca agcgattcaa acattaaaaa tcgtaaaaga tttatttgga
     2761 agttcaatag ttggagtata tctatttggt tcagcagtaa atggtggttt acgcattaac
     2821 agcgatgtag atgttctagt cgtcgtgaat catagtttac ctcaattaac tcgaaaaaaa
     2881 ctaacagaaa gactaatgac tatatcagga aagattggaa atacggattc tgttagacca
     2941 cttgaagtta cggttataaa taggagtgaa gttgtccctt ggcaatatcc tccaaaaaga
     3001 gaatttatat acggtgagtg gctcaggggt gaatttgaga atggacaaat tcaggaacca
     3061 agctatgatc ctgatttggc tattgtttta gcacaagcaa gaaagaatag tatttctcta
     3121 tttggtcctg attcttcaag tatacttgtc tccgtacctt tgacagatat tcgaagagca
     3181 attaaggatt ctttgccaga actaattgag gggataaaag gtgatgagcg taatgtaatt
     3241 ttaaccctag ctcgaatgtg gcaaacagtg actactggtg aaattacctc gaaagatgtc
     3301 gctgcagaat gggctatacc tcttttacct aaagagcatg taactttact ggatatagct
     3361 agaaaaggct atcggggaga gtgtgatgat aagtgggaag gactatattc aaaggtgaaa
     3421 gcactcgtta agtatatgaa aaattctata gaaacttctc tcaattaggc taattttatt
     3481 gcaataacag gtgcttactt ttaaaactac tgatttattg ataaatattg aacaattttt
     3541 gggaagaata aagcgtcctc ttgtgaaatt agagaacgct ttattacttt aatttagtga
     3601 aacaatttgt aactattgaa aatagaaaga aattgttcct tcgatagttt attaatatta
     3661 gtgacatttg catgcttcaa agcctgtcgg aattggtttt tagtgaaaag aacacgatat
     3721 tcacggttta cccacttata aacaaaagat cgatactttt tgtagtcctt ctttgaaatc
     3781 aatggttgat gtcgttcaag aacaatcaat acagagtcta cacttggctt aggatgaaaa
     3841 tatagtggtg gtactttttt gagcattttt atatccatct ccaccattaa tagtaaaccc
     3901 aaagctcgtt gcagattttg caatcttttc gcaaatccct tctcaacgat aagatagcta
     3961 tatttagcct gactttcaaa ggtaattctt ttgacaatat ccgtactgat gttataagga
     4021 atattaccat atatcttata gtttatatgt tttgggaagg aaaattttag aatatccgtt
     4081 tgaatcactt ttatattctc agaggggttt accgcttctt tagtcacttg acataagcct
     4141 ccatcaattt ctatagcagt aactgatcga ctcattttga ctagctcttt ggtaaaatgt
     4201 ccttttcctg atccgatttc tattacgttg tcttgtttac tgatattcgt gtgattcaat
     4261 atttctttta catgcttttt agaagtaata aaattttgcg tgtctttagg gtttttctgg
     4321 ttcattataa ccttctcctt actggttata atgaactgac attaagcgtt cattataacc
     4381 aattaatttt gatttggttg ataacgaact ttattaataa caaatataga aaaagtaccc
     4441 atacttttat ctcctttcca tttttttcat tatgaatgag ataaagtaat ttctactact
     4501 gcgatactgg tgcacataat tgaaacctcc ttatgtaact gaatatgatt gtatcatctt
     4561 ttcattttag aataaacctt tatatgtaat tttgcacttg acacttcaga tggacgtttt
     4621 cttcaaaata atatgttttg aaacttgata agagaagcca ctgtctttac taaaaagtaa
     4681 aggctgcgat aaaggacagt gactttaata aagttgcact gttgtattgt ctttggataa
     4741 aattgtctaa agggaagttt tatccccaac gaaaaaacat ctaaagtggt aagtaatgaa
     4801 agaatgtcga taaagtagga atgggggatc tttttgaata caatagaatc acagtttgat
     4861 aaggttgcag aagattacga tttcgtgaat gagcttttga atgattattc attctttgtg
     4921 tctaatatgt ctccaaagaa aggcagagca ttagatatcg gatgtggctc gggtttgtta
     4981 gtggagaaat tagcaagtta ttatgatgaa gtggtaggga ttgatatttc taatcaaatg
     5041 ctcgatcttg ccaaatctaa acgtcaacta acaaatacgg tctatctgaa tatgaatgca
     5101 gaacaactta attttaatga gaagtttgat tttattgtaa gccgaacaac ctttcatcat
     5161 ttggatgata tagccagcgt aatacagcaa atgaaggaac tgttgaatga ggaaggaaga
     5221 atagttattc ttgataatgt atctgaagtc gagacaccgc ctacctatgt ttataaatta
     5281 ggggcaatcc aagagttttt accgcattgt ttcaaatttg gaataaaaaa tgcgattaga
     5341 atttataatc acaacacatc taaatcatgg cttgagcatt tagcttcaga taagtaccta
     5401 tctgaacaaa attattacga tttatatgaa aagttgttgc ctggatgcca attccataaa
     5461 atgggttggg caatgggggt tgtctggaca aaataactta aagctgtggc tttgaaaagt
     5521 tgaatttgtg attgttttca gagctatcgg gtgctttagt ataaataaca atgaataaaa
     5581 aatcagcctg tatcttatgt tactatta
//
LOCUS       DARBET010000248         2024 bp    DNA     linear   BCT 07-NOV-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4027
            SAMEA114569841-rid20112183.guided.168, whole genome shotgun
            sequence.
ACCESSION   DARBET010000248 DARBET010000000
VERSION     DARBET010000248.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA114569841
            Sequence Read Archive: ERR12203666
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2024)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2024)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001972618.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001972618.1
            Genome Coverage       :: 132x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/07/2023 13:18:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,041
            CDSs (total)                      :: 2,975
            Genes (coding)                    :: 2,908
            CDSs (with protein)               :: 2,908
            Genes (RNA)                       :: 66
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 67
            CDSs (without protein)            :: 67
            Pseudo Genes (ambiguous residues) :: 0 of 67
            Pseudo Genes (frameshifted)       :: 26 of 67
            Pseudo Genes (incomplete)         :: 28 of 67
            Pseudo Genes (internal stop)      :: 20 of 67
            Pseudo Genes (multiple problems)  :: 6 of 67
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2024
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA114569841-rid20112183.guided.168"
                     /isolate="VRE4027"
                     /db_xref="taxon:1352"
                     /country="Denmark"
     gene            complement(680..970)
                     /locus_tag="SCJ86_003038"
     CDS             complement(680..970)
                     /locus_tag="SCJ86_003038"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295437.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEG1170502.1"
                     /translation="MEKKYGTVDKIRTLQVSARPLIRFSLNGVNCLIASHSLNFLAEV
                     DEGMKLVVTGYYNNRKQFVVRSYHVLGKPKIVVEYEKSLYPRKKVRQDERIN"
     gene            complement(1178..1870)
                     /locus_tag="SCJ86_003039"
     CDS             complement(1178..1870)
                     /locus_tag="SCJ86_003039"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318394.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit omega"
                     /protein_id="HEG1170503.1"
                     /translation="MKKKFITGGIVVAFLCLIGYTGTRYANEIILWGGETDIDTINEN
                     LTQLDHTLTDKEQALTDLTARLAQKEQTLQDSKADVQEYQTKVNELENQKNQSAAEKQ
                     NLESQLVSKDAQLQSKQNEINVKLDEINQKNDEINHLNADWTARLQEAQAKLSELQNT
                     VNGLNQQIATLTQEKTNVETQLTDTQKKLDEATAENSSLKQYIQKLEKAKDEVEDTAD
                     KSQQIVEEHLRK"
BASE COUNT          562 a          382 c          301 g          779 t
ORIGIN      
        1 taacatagct ttaagcatta cttaaagata acttaaaagt tatcttttaa atacttaatt
       61 gttaaatacc ttatttgatg caaaagaaat ctacgatagt caacaaaaaa attttcatta
      121 tatccagaaa aatttttctt agttaaggac ctatggattg cagtaatgca gaattcgtcg
      181 aataaactgc caaagacata caatcatatt gcttttaaaa ttgacgaaca agatatagat
      241 tcgtttgtct caaaaatcca aatgttaggt ttaactgtcg aaccaggacg ttctcgtgtg
      301 aaaggcgagg cttcttcaat ttacttttac gactatgata atcacctctt cgagttacat
      361 accggtactc tccaggaacg tttaaaatcc tacaattcca ctacctgaga ctagtaatga
      421 ctagtctctt tttatggcct ctgatccccc cattgagcga atactaatag ggcttcgaat
      481 tgatctgttc aatctttaag caatatccct caaatgagtt tggaaagttt agtgattcat
      541 cacaaatact taaatacatg tagttatata tagattgagg ttcttgtgag cgtatgctcg
      601 tgtgggttca aatcccatcg tctgcatcca tctttccaaa acaagcggct aggacatttt
      661 tgtctcagcc gcttgttttc tagtttattc tttcatcttg tcggaccttt tttcttgggt
      721 atagactttt ctcatattct accacaattt tgggtttccc taacacatga taggatctca
      781 caacaaattg ctttctatta ttataataac ctgtaacgac taacttcatc ccttcgtcta
      841 cttcagctag gaagttcaaa ctatgggaag caatcaaaca attgacccca tttaaactga
      901 aacggatcaa cggtctggca ctaacttgca aagtacggat cttatctact gttccatatt
      961 ttttctccat aaccttcacc tcaataccat tatacgaaca tacattccct ttgtaaagaa
     1021 gaaactctgt tttatctttt tgactttttg tctattacct ttcttaaaat catattggca
     1081 cttcctgcga agatgaagga catgtactaa aaaaaagagg cagcagtata gattactgtt
     1141 gcttctttac taatttaggt ttacttgtta agaaagatta tttacgcaaa tgttcttcaa
     1201 caatttgctg agatttatca gctgtatctt ccacttcatc ttttgctttt tctaactttt
     1261 gaatatattg tttcaagctt gagttttcag cagttgcttc gtctaatttc ttttgtgtat
     1321 ctgttaactg agtttctaca tttgtttttt cttgtgtcag tgttgcgatt tgttgattca
     1381 aaccatttac agtattttgt aattctgaaa gttttgcttg tgcttcttgt agtcttgctg
     1441 tccaatctgc attcagatga ttgatttcat catttttttg gttgatctca tctagtttga
     1501 cattgatttc attttgtttg ctttgtagtt gtgcatcctt gctgactaat tgactttcta
     1561 agttttgttt ttctgctgcc gattgatttt tttggttttc taattcattt acttttgttt
     1621 ggtattcttg gacatctgcc tttgaatctt gcaatgtctg ttctttttgt gctagtcgag
     1681 cggtaagatc tgtaagcgct tgctctttat ctgtcaatgt atgatcaagc tgtgtcagat
     1741 tctcattgat tgtatcaata tctgtctctc caccccacaa aataatttca tttgcgtacc
     1801 gcgtgcctgt gtagccaatt agacatagaa atgcaacgac aatcccacca gtaataaatt
     1861 tctttttcat ttgcatagct ccttaatcca atacttttac atgctaaaag taagtgatct
     1921 caaacacaat gtcaagcgtt tttaaattac atgtgtaatt ttaatataaa tactatcaaa
     1981 tactcattta atcctccttt tttatctatt taaaaaatct tttt
//
LOCUS       DARBET010000249          733 bp    DNA     linear   BCT 07-NOV-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4027
            SAMEA114569841-rid20112183.guided.226, whole genome shotgun
            sequence.
ACCESSION   DARBET010000249 DARBET010000000
VERSION     DARBET010000249.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA114569841
            Sequence Read Archive: ERR12203666
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 733)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 733)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001972618.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001972618.1
            Genome Coverage       :: 132x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/07/2023 13:18:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,041
            CDSs (total)                      :: 2,975
            Genes (coding)                    :: 2,908
            CDSs (with protein)               :: 2,908
            Genes (RNA)                       :: 66
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 67
            CDSs (without protein)            :: 67
            Pseudo Genes (ambiguous residues) :: 0 of 67
            Pseudo Genes (frameshifted)       :: 26 of 67
            Pseudo Genes (incomplete)         :: 28 of 67
            Pseudo Genes (internal stop)      :: 20 of 67
            Pseudo Genes (multiple problems)  :: 6 of 67
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..733
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA114569841-rid20112183.guided.226"
                     /isolate="VRE4027"
                     /db_xref="taxon:1352"
                     /country="Denmark"
     gene            <1..662
                     /locus_tag="SCJ86_003040"
     CDS             <1..662
                     /locus_tag="SCJ86_003040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_021424053.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="aminoglycoside phosphotransferase family
                     protein"
                     /protein_id="HEG1170504.1"
                     /translation="EYSYISDELSILGYKEIKGTFLTPEIYSTMSEEEQNLLKRDIAS
                     FLRQMHGLDYTDISECTIDNKQNVLEEYILLRETIYNDLTDIEKDYIESFMERLNATT
                     VFEGKKCLCHNDFSCNHLLLDGNNRLTGIIDFGDSGIIDEYCDFIYLLEDSEEEIGTN
                     FGEDILRMYGNIDIEKAKEYQDIVEEYYPIETIVYGIKNIKQEFIENGRKEIYKRTYK
                     D"
BASE COUNT          307 a           52 c          125 g          249 t
ORIGIN      
        1 ttgaatattc gtatattagt gatgaattat ctatactagg ttataaagaa attaaaggaa
       61 cttttttaac accagaaatt tattctacta tgtcagaaga agaacaaaat ttgttaaaac
      121 gagatattgc cagtttttta agacaaatgc acggtttaga ttatacagat attagtgaat
      181 gtactattga taataaacaa aatgtattag aagagtatat attgttgcgt gaaactattt
      241 ataatgattt aactgatata gaaaaagatt atatagaaag ttttatggaa agactaaatg
      301 caacaacagt ttttgagggt aaaaagtgtt tatgccataa tgattttagt tgtaatcatc
      361 tattgttaga tggcaataat agattaactg gaataattga ttttggagat tctggaatta
      421 tagatgaata ttgtgatttt atatacttac ttgaagatag tgaagaagaa ataggaacaa
      481 attttggaga agatatatta agaatgtatg gaaatataga tattgagaaa gcaaaagaat
      541 atcaagatat agttgaagaa tattatccta ttgaaactat tgtttatgga attaaaaata
      601 ttaaacagga atttatcgaa aatggtagaa aagaaattta taaaaggact tataaagatt
      661 gattatataa tatatgaaaa gctattataa aagacggttc tgttgcaaag ttttaaatct
      721 actatcaaat aag
//
LOCUS       DARBET010000250          689 bp    DNA     linear   BCT 07-NOV-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4027
            SAMEA114569841-rid20112183.guided.229, whole genome shotgun
            sequence.
ACCESSION   DARBET010000250 DARBET010000000
VERSION     DARBET010000250.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA114569841
            Sequence Read Archive: ERR12203666
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 689)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 689)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001972618.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001972618.1
            Genome Coverage       :: 132x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/07/2023 13:18:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,041
            CDSs (total)                      :: 2,975
            Genes (coding)                    :: 2,908
            CDSs (with protein)               :: 2,908
            Genes (RNA)                       :: 66
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 67
            CDSs (without protein)            :: 67
            Pseudo Genes (ambiguous residues) :: 0 of 67
            Pseudo Genes (frameshifted)       :: 26 of 67
            Pseudo Genes (incomplete)         :: 28 of 67
            Pseudo Genes (internal stop)      :: 20 of 67
            Pseudo Genes (multiple problems)  :: 6 of 67
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..689
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA114569841-rid20112183.guided.229"
                     /isolate="VRE4027"
                     /db_xref="taxon:1352"
                     /country="Denmark"
     gene            <1..>689
                     /locus_tag="SCJ86_003041"
     CDS             <1..>689
                     /locus_tag="SCJ86_003041"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012655884.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEG1170505.1"
                     /translation="RDFILYLKLCNRTESLKKHYTEPWEDEVFRVIIEYNNVPIGYGQ
                     IYKMYDELYTDYHYPKTDEIVYGMDQFIGEPNYWSKGIGTRYIKLIFEFLKKERNANA
                     VILDPHKNNPRAIRAYQKSGFRIIEDLPEHELHEGKKEDCYLMEYRYDDNATNVKAMK
                     YLIEHYFDNFKVDSIEIIGSGYDSVAYLVNNEYIFKTKFSTNKKKGYAKEKAIYNFLN
                     TNLETNVKIPN"
BASE COUNT          290 a           62 c          114 g          223 t
ORIGIN      
        1 agggatttta ttctttattt aaaactttgc aacagaaccg aatcattaaa aaaacattat
       61 acagagcctt gggaagatga agtttttaga gtaattattg aatataacaa tgttcctatt
      121 ggatatggac aaatatataa aatgtatgat gagttatata ctgattatca ttatccaaaa
      181 actgatgaga tagtctatgg tatggatcaa tttataggag agccaaatta ttggagtaaa
      241 ggaattggta caagatatat taaattgatt tttgaatttt tgaaaaaaga aagaaatgct
      301 aatgcagtta ttttagaccc tcataaaaat aatccaagag caataagggc ataccaaaaa
      361 tctggtttta gaattattga agatttgcca gaacatgaat tacacgaggg caaaaaagaa
      421 gattgttatt taatggaata tagatatgat gataatgcca caaatgttaa ggcaatgaaa
      481 tatttaattg agcattactt tgataatttc aaagtagata gtattgaaat aatcggtagt
      541 ggttatgata gtgtggcata tttagttaat aatgaataca tttttaaaac aaaatttagt
      601 actaataaga aaaaaggtta tgcaaaagaa aaagcaatat ataatttttt aaatacaaat
      661 ttagaaacta atgtaaaaat tcctaatat
//
